LOCUS       DAOPQX010000001        34071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_1681_length_34071_cov_4.732626, whole genome shotgun sequence.
ACCESSION   DAOPQX010000001 DAOPQX010000000
VERSION     DAOPQX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 34071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34071
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1681_length_34071_cov_4.732626"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1886)
                     /locus_tag="PKK95_00005"
     CDS             complement(<1..1886)
                     /locus_tag="PKK95_00005"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOC16613.1"
                     /translation="MKDAAVYSLIRRGGAAWLRRPLVSVGAALVALVLVAAPAALAAA
                     APSPQVAAEATEPAHVGGEANLVLPDLGAVDFMGVNGRTLLMVGLLVCVGGLLFGLVI
                     YRQLRALPVHASMRDISELIYETCKAYMLQQGRFLLILEAFIGVIIVVYFGFLQNFEW
                     SRVGIILAFSVIGIAGSYGVAWFGIRLNTLANSRTAFASLRGKAFPIYLIPLKSGMSI
                     GMALISVELFMMLSILLFIPREYAGPCFIGFAIGESLGAAALRIAGGIFTKIADIGSD
                     LMKIVFNIKEDDARNPGVIADCTGDNAGDSVGPTADGFETYGVTGVALITFIVLAVPD
                     ATVQVQLLVWIFVMRIMMIVASGLSYLVNNTFARARYGNAERMNFEAPLTSLVWITSI
                     VSVIITYIVSFLLIPDLAGDGSLWWKLSTVITCGTLAGAIIPELVKIFTSTESAHVKE
                     VVRSGREGGASLDILSGLVAGNFSAYWLGLAIMLLMAIGYVVSTTFPGGLMMAPAVFA
                     FGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVFELSVIEQIPGIKQELRRDHGIDADFG
                     AAKDLLETGDGAGNTFKATAKPVLIGTAVVGATTMIFSIILALTNGLRDNVDKLSILN
                     PPFLL"
     gene            complement(1973..2635)
                     /locus_tag="PKK95_00010"
     CDS             complement(1973..2635)
                     /locus_tag="PKK95_00010"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOC16614.1"
                     /translation="MRPVLWLRRRFVTGFFVAVPLIVSVAALIWAFDFIDGITRPLYD
                     RWLGRHVPGLGLVTTALFLLLVGVAASNVLGNRLVLGLERSLLRLPLFRTVYSPVKQL
                     IEAFSPDNAAGFKRVVIVVDRTGVRRLGFLTREFELSSGEGTVGLVAVFVPTNHLYIG
                     DVYFYPPEWVVLPDLTVEEGLRIVLTGGTAVPKQLTGRPLAGVAARGAGVPEQTDIES
                     AG"
     gene            complement(2649..3458)
                     /gene="uppP"
                     /locus_tag="PKK95_00015"
     CDS             complement(2649..3458)
                     /gene="uppP"
                     /locus_tag="PKK95_00015"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOC16615.1"
                     /translation="MGSVLAALLGVVQGLTEFLPVSSSAHLILARAFFGWDAGRMGLA
                     FDVACHVGTLLAVVVYFWRDLVGMARALPGLFDRHPSPPARLARLIVVGTIPAVLAGL
                     LFGDVIEDRLRTPVVAAVTLGVVAVSFFAVEALGRRTRAHGTLTAAEALGIGVAQASA
                     LVPGVSRSGATIVVGMVLGLRREEAARFAFLLGIPAILAAAGREGVMLLERGLSGGEA
                     QLFAVGMASSAVVGYFTVKYFLRYLTGHSLAIFAWYRLLLAGAVVVWLFVR"
     gene            complement(3468..4466)
                     /gene="secF"
                     /locus_tag="PKK95_00020"
     CDS             complement(3468..4466)
                     /gene="secF"
                     /locus_tag="PKK95_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOC16616.1"
                     /translation="MQIFHDPNYNFVRYRWPAIILSTVLILAGVAALFLRGLPLGVEF
                     SGGTIVIVQFDTVPSIDDVREAVGGRFGGGQDLVVQSYGAASERRIMIRVPHVGREEG
                     AELSRPAQQIEEALRAANVGGFQVSSTEVVGPIVGRDLTRKGLLAFSLSLFFILLYIW
                     FRFQLSFATAATIATLHDLLVTLAFLVFFGFDISLNVIAALLTITGYSTNDTIVIFDR
                     VRENLRGTRRDDITHLVNSSINQTLNRTIITGGTALFACVFLYFFGGEVLKGFAFTMV
                     VGMIVGTYSSIYIAAAIVTFWPHGKGGARIAASAPAAAKAATTARQRKAGRGARAS"
     gene            complement(4469..6028)
                     /gene="secD"
                     /locus_tag="PKK95_00025"
     CDS             complement(4469..6028)
                     /gene="secD"
                     /locus_tag="PKK95_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOC16617.1"
                     /translation="MNNLRWRILLIVVIVGACLWAIIPPEQKIRLGLDLKGGVHLVLQ
                     VNTDDALRLDTETTAESLRNELEKSGLTSATVEATSPTEFRVNGLQPGQDAVLRQAAT
                     ALATGWDRESGVGGGYVFRMRPARQNQLRDESVRQAMDTIERRVNDLGVAEPVVARHG
                     GTEGEQLLVQLPGISDVARAKEIIRSTAMLELKLVIQGPASTKEGLLAATGGQVPPHA
                     EVLPGVQAGTDGRAETVYYLLEKAPIVTGRDLRNAKPTLDEYNQPAVSFSLNPEGARK
                     FGRATGENVGRQLAIVLDNQVRSAPRIDERITDEGRITGGFTQQEAQDLSLVLRSGAL
                     PASLTYLEERTIGPSLGADSIKAGIVASSLSLVVVALFMLFYYRLSGINALVALLFNL
                     IILLGAMSYVGATMTLPGIAGFILTMTMGVDSNVLIFERIKEELAAQRGVRASINAGF
                     SRVFLTLVDTHLSALIGAAFLFQFGTGPIRGYATTLFFGLVSNLFTSTFVSKTLFEAI
                     LSKRQVESLSI"
     gene            complement(6025..6369)
                     /gene="yajC"
                     /locus_tag="PKK95_00030"
     CDS             complement(6025..6369)
                     /gene="yajC"
                     /locus_tag="PKK95_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOC16618.1"
                     /translation="MVLHACTLVAMAAPADSGVSPWVQFVPFLLVLAIFYFIILLPMK
                     RRQKKVQTFQEALKVGDRVITTAGIHGTITRLGDRTVKVQVADKVQIEFSRAAIAGYQ
                     GQDPVVADDSQA"
     gene            complement(6375..7532)
                     /gene="tgt"
                     /locus_tag="PKK95_00035"
     CDS             complement(6375..7532)
                     /gene="tgt"
                     /locus_tag="PKK95_00035"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOC16619.1"
                     /translation="MAPVFTFRLTHTCGEARRGEMVTAHGPVQTPAFMPVGTQGAVKG
                     VLPRDLHEMGAEILLANTYHLSLRPGDARIARLGGLHAFSGWDRAVLTDSGGFQAFSL
                     GDRRTIDEEGVRFRSHLDGSPHQLTPEGAADIQARLGSDIAMVLDECLAFPAERDHAA
                     ASMERTARWAARARARFLELSSGAAGEMTNPGQAQFGIVQGGVFPDLRRVSAEATVAV
                     GFEAYAIGGLSVGEPAGQMYDVVACTAPLLPADRPRYLMGTGTPLDLVEGVARGIDLF
                     DCVLPTRNARNGQLFTSSGRLNIKNAAFADDPAPIEEGCGCYACRHFSRAYLRHLHLA
                     GEIGASTLNTLHNLWFYLDTMRRIREALVFGSFDRLRQDVRQVYSPRVADS"
     gene            7584..8744
                     /locus_tag="PKK95_00040"
     CDS             7584..8744
                     /locus_tag="PKK95_00040"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOC16620.1"
                     /translation="MKKLRIGVIYGGRSSEHEVSLASAASVIANLDRSRYDPVPIRID
                     RDGRWTIADRPPTTIAASAVIAKARQEPPRSTRTGREAYLVPHPGEETVLTIERNAGE
                     ATNGRENAYVAGLGLDVVFPVLHGPYGEDGTVQGLLELANVPYVGSGVLASAVGMDKA
                     MMKVCFAARGLPIVKHLVAWRDEWAREPDAIASRVGAELRPPVFVKPANLGSSVGISK
                     AGDRAALGRAIELAAQYDRKIVIEEAVPAAREIECSVVGNARPEASVPGEILPSREFY
                     DYEAKYLEDSPTLVPAPLDEAETARVRQLAVEAFLAVEASGYARVDLLASRETGDIFV
                     NEINTIPGFTTISMFPKLWEATGLPYPALLDRLVELAFERHAAKQALRTGRP"
     gene            8741..10264
                     /locus_tag="PKK95_00045"
     CDS             8741..10264
                     /locus_tag="PKK95_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16621.1"
                     /translation="MKRRAAAALVVVVAALGAGPARAAEPSYFPLLGRAYDAVLDARF
                     DEVEPALVEACGPAPEVACDLVRAVAVWWRIQLDPLDRSRDAEFQRRVDAVIEAAEQW
                     SRREPKSAEAYFFLGAACGARVQWRVLRGERLAAARDGKRIKESLERALALDPTLEDA
                     YFGIGMYRYYADLVPTVLKFFRWLLFLPGGDRERGLAEMQQAREQGTLLRGETDFQLH
                     LVYIWYEDRVDEALALLADLRRRYPHNPLFLQAAAEIHDVYRHDAPASLDAWRRLLNL
                     ARARRVSLPEESEARARVGMAEALDDLYQTDYALEQLRIVTESSPASPYGLAARAWLL
                     LGEAHDRLGGREAAVAAYRAAMAAAPRDDPDRVKSRASEALRRRPDPRAAEAYRRSLE
                     GWRHLQRGELDPAAEALSRAAALNPADPVLRYRLGRLHLALHQRDEALAQFERAIAAR
                     PAAPPALLAAALLEAARLLESRGDGERALSLYKRAAALRGAEPDTRRAAARAVSRLQ"
     gene            complement(10842..12986)
                     /locus_tag="PKK95_00050"
     CDS             complement(10842..12986)
                     /locus_tag="PKK95_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC16622.1"
                     /translation="MTCHLRTPACLRPALAARLVVVLPALLAGCATAAPPPAPVIAME
                     QKLSWILRLEDQRALRDPAPAPAIAPRRGRTLPPPPPSPDLLALVRDDLARVRRSAAL
                     AIGRVGLPEGVPVLSATLESDPDPAVRQAAAFALGVLGDAGGATPLRRALDDPDPRVQ
                     GRAAEALSLLGQADAAVDIGRMVARQLESSNVAALEPDAPPEGQEPAADAFRLGVVAL
                     GCLKAYEPLAAAVLDPGGKPRVGWWPVAFAFQRTGDRRALPALLAFARAGGSFGRSFA
                     ARGLGSLKDPAASPALTAMADLWRDDPRAAVSAIRALGQVGDPSARETLARLLSTRDL
                     DPVALVEAIAAAGAARAREAQERVQDLTGHPSPAVRAAALRALREIDEESFTIVLSGL
                     DADRHWSVRAATATVLGTLAADRAVPRLTAMLADPDARVAPAVLRALVQLRAPGIEAR
                     VTEALGHQDVVVRMTAAAALGELKPASGDGRLAEAYRSWAGDDTYIARTAALEALAKY
                     GERARAVLEEALADREWPVRLKAAALLRSLDPAAAAVPARPVPTGRPDSWYEAPELVS
                     PSVSPQVYLETARGVVQLELLVVDAPMTCANFAALARRGYFNGLLFHRVVPDFVVQDG
                     DPRGDGEGGPGYTIRDELNERAYGRGTVGMALDGPDTGGSQYFITHSPQPHLEGRYTV
                     FARVVAGMEVVDRLQPWDEIVRVRVWDGQTFE"
     gene            13087..13722
                     /locus_tag="PKK95_00055"
     CDS             13087..13722
                     /locus_tag="PKK95_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOC16623.1"
                     /translation="MDFRSIAFEGPPGVGKTALAERVAARLDATTVLEETENPFLADF
                     YGDRPGAGLQAQLFFLLGRHRQQVALRQADLFSQITVCDYLFEKDRIYAFLNLDDNEL
                     FIYQRLYDLLARDVAPPDLVVYLQAPTDLLLKRLRARDEQQPYRLNPEYIKKLIEAYQ
                     HFFFHYSATPLLVVETSQLDLGGSDEALDDLLRQIRGMGRGTRYYVPRTRD"
     gene            13755..14594
                     /gene="accD"
                     /locus_tag="PKK95_00060"
     CDS             13755..14594
                     /gene="accD"
                     /locus_tag="PKK95_00060"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOC16624.1"
                     /translation="MAWFKKTRKPIDPAVKASRVPEGLYVKCPGCSELLYNKDLAANL
                     SVCGKCGHHFRMTAAERLAMLFDGPWTEHDAGLVSTDPLGFKDTKSYQDRLRAAAAAT
                     GVKEAVVTATGQLDGRPAVIAAMEYGFIGGSMGVVVGEKIARAIERAIEGRAAFIAVS
                     CSGGARMMEGALSLMQMAKTSAALARLDRAGLPFLSILTDPTTGGVTASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTIRQKLPEGFQRSEFLLEHGMVDLIADRRELKAVIARALRF
                     MLA"
     gene            14591..15916
                     /locus_tag="PKK95_00065"
     CDS             14591..15916
                     /locus_tag="PKK95_00065"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOC16625.1"
                     /translation="MTSPSDPVAALLALEKLGIKFGLDNIRAICDALDHPQDAWSSVL
                     VAGTNGKGSVAAMVEAGLRASGRRTGLYTSPHLIRLEERFAIDGRPVDPDRMRSAASR
                     VLRAIASARARGALLTDPTFFEATTAVAFDLFREAGVEVAVLEVGLGGRFDATNLVVP
                     AAGAITTIDLDHQQLLGHTLAAIAAEKAGIVKPGMRLVVGERKPEAVDVIAAACASQG
                     ATMIPAWDGVAAAVATRAGGLASMDAGVSLSLRTPRHDYGEVSLSLRGRHQADNAVVA
                     VRLLEALGPAGLHVPPGAVQAALTSTRWPGRLDLRRLDGGRSVLLDAAHNPAGAGVLA
                     SYLSEVAPSGLPLVFAIMKDKDAPGTLAPLLPYARPLIVTRPSTPRALAPEEVARIAE
                     RLGYRVPVEVEPEVGAALDAAWRRAGSIGACGSIFLVGEALERLGLPAW"
     gene            15996..18350
                     /locus_tag="PKK95_00070"
     CDS             15996..18350
                     /locus_tag="PKK95_00070"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOC16626.1"
                     /translation="MSRIVLPLLVARPRRAALALALASVLVWPAGAAAQAPRPGATSS
                     RETGFDTSKQWRIEQPAKNHLRLIGEVEARRGDATFTADLIESFTDTHEVVATGSPGR
                     PVVFTQGGQWIAAERVEFNTETQLGTFYEAYGSFTVASEPKRSMHGGQEPDVLFNGRK
                     IEKVGPKKYRITDGAFTTCLQPTPRWNVSAGTLVLNLDRYAFLRGAVLRAKGLPVLYL
                     PFLYYPINDEDRATGFLMPSYGTSTYRGFTLSNAFFWAIGRSHDATFLHDWYAKRGQG
                     LGTEYRYVGPGGAGGDLRFYTLRERESTITSGGVESLLPKRTSYEVNGNINQPLGGSW
                     TARGNVDYFSDIEVQQTYHTNIYDASRSQRSVNAGLSGTLAQLTVNANYRRSEYFSGR
                     SNTTVVGGAPQIQITRGEQPLFGLPLYFGVTGEYAKLQREYHRTSGVTDSNLSRLDVT
                     PTLRFPFTKLRWLTVNTSLAFRETWWSRSRVADTNDFVDDGISRRYAQLSARLVGPVL
                     NRVFDTPGFGYAERLKHTVEPYVDIRRVTRVDNFDEIIQNDGTDSVVGGTTSITYGVN
                     NRLLAKRRGGEGPSSPREFVTVGLSQTYYSDSRASQFDRSYSTSYSGLPPSKRSPVSL
                     TARVTPTREVNGSLRMEYDDRAKKIRSLSASWQYAASGWLNLVGGYSQRRVIDYAEST
                     VTETKYRLDNSINLSTTLRSPANRVGGSYTFNYDIDRSNLLSSRVIGYYNAQCCGFAV
                     EYQTVTYGSSSPIGKDNRFNFSFTLAGLGTFSNFFGALGGGGVR"
     gene            18366..19433
                     /locus_tag="PKK95_00075"
     CDS             18366..19433
                     /locus_tag="PKK95_00075"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HOC16627.1"
                     /translation="MKGLILSGGKGTRLRPLTYTSAKQLVPVANKPVLFYGIEAMAEA
                     GIREVGIVVGDTAPEIEAAVADGSRWGLRVTYIPQDAPRGLAHAVLISEGFLGGEPFV
                     MYLGDNLLNKGIGPFVRQFEAERPAAQILLARVSDPQMFGVAELEGGRVVRLVEKPRE
                     PKSDYALVGVYMFGPEIFTSVRRIRPSWRSELEITDAIQDLIDRGLEVRPHVVDGWWK
                     DTGKLEDLLEANRLILDTLERRIEGEVDEASRIEGKVVVEPGAAIERSVVRGPVVIGS
                     GARIAQAYIGPFTSIMNDVQVRDAEIEHSIVLEGSEICGLASRIEDSLIGKNVRIYRM
                     PVKPSAYRFMLGDNSEVGIKW"
     gene            19427..19939
                     /locus_tag="PKK95_00080"
     CDS             19427..19939
                     /locus_tag="PKK95_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOC16628.1"
                     /translation="MVNDITKEPAPYVPPGKRARIDGVKTKALRIIPDDRGWLMEILR
                     ADEADLFERFGQVYVSATYPGVVKAWHYHKRQTDNFACVQGMVKLVLVDTRPGSPTEG
                     VVNEFSIGTLNPMLVQVPRLVFHGWKCTGTEPALVVNAPTEPYRYDEPDEYRLEPHGT
                     LPYDWSRRDG"
     gene            19932..20939
                     /gene="rfbB"
                     /locus_tag="PKK95_00085"
     CDS             19932..20939
                     /gene="rfbB"
                     /locus_tag="PKK95_00085"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOC16629.1"
                     /translation="MVEVLVTGGAGFIGSQFVRSALAHPDWRVTTLDKLTYAGRLENL
                     QEVLDHPRHRFVRGDVCDPGLVGPLVGSAEIVVHFAAETHVDRSIQAAGDFIRTDVYG
                     TFVLLEAARTAPRLRRFVQISTDEVYGSVPEGASRETDELRPRNPYAASKAGADRLAY
                     SYWATYRVPVVVTRASNNYGPRQFPEKLIPLFVTNAMDGLPVPLYGDGLNVRDWLHVE
                     DHCRALDLLIERGTPGEVYNIGGGNLVPNVDLTRRILALLGRPESLVQPVADRPGHDR
                     RYCLDTSKLRGLGWEPSVPFEGGLEATVDWYRTNEWWWRPIKQGDPAFRTYYEQQYGR
                     R"
     gene            20936..21886
                     /locus_tag="PKK95_00090"
     CDS             20936..21886
                     /locus_tag="PKK95_00090"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOC16630.1"
                     /translation="MNGPILVTGAAGFAGSHLLDLLGAADVPVVAWRRPGEALPPPPT
                     GGACRWMEVDVLDRDNVEHAVREAAPSAVYHLAGAAHVGTSWDDSAATLAVNVLGTWH
                     LLASLAALPGRPRTLVSGSALVYARQDRAISEDDPVGPASPYAVSKLAQELAAAHAVR
                     ELGLPVVITRSFNHIGPRQSPSFSSASFARQIALAEAGRGPRELLVGNLDPRRDLTDV
                     RDIVAAYRALLDAGAPGRVYNVCSGHAVRVRDVLDGLAALARVPIDVRVDEALLRPHD
                     TPLVLGNPRRIREEIGWEPRIGLADTLRDLLDYWREAILR"
     gene            21883..23226
                     /locus_tag="PKK95_00095"
     CDS             21883..23226
                     /locus_tag="PKK95_00095"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HOC16631.1"
                     /translation="MSGFSESRRQAIHMSMALFALALRYLAWWQAAICAVAAFLFNLL
                     VLPRLGGSSLYRPSDQARGYPLGILFYPLSVLTLILAFPRRPDIVAASWGILAVGDGL
                     ATIVGRRAGQRRLSWNPEKTVAGSLALALGGGAAGVVLTAWTRPAVSPEPPLAFVLLA
                     PFLAGAAAALVESLRVRLDDNISVPIAAAIVLGALGLMDAESAGAAAAWLPRAFPLAL
                     AVNVPVALLGWRAGSVSRSGAIAGAVIGILVLTFAGPAGWALLFVSFLLATASTRLGL
                     KRKAVLGIAEERGGRRGPGNAIANTGLAALAAAVAALSPYRDGALLVMVAALAAGASD
                     TVASEVGKAWGRRTFLFPSLRPVKPGTSGAVSLEGTAAGLVSALALSSLATALGLITG
                     NALWFAAIGATFGAFVESGLGATLEPGGILDNDMLNFINTGAAGAAAVALSWISR"
     gene            23211..24143
                     /locus_tag="PKK95_00100"
     CDS             23211..24143
                     /locus_tag="PKK95_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOC16632.1"
                     /translation="MDLQVRPVRARATTWLEFARPFTLFAPALGMASGGVAAIGAQPP
                     EPWAWHLVAYPLLGAAAAAVLNAASNGLNQIYDLEIDRVNKPRRPLPSGRMSVGEAWA
                     FTIAAFAISWLLAWTIAPGGRRHTFWMFVAASLITALYSVPPFRTKRLGVWANVTVAI
                     PRGALLKVAGWSTVKTAFGWEPWFIGAIFGLFLLGATTTKDFADMKGDALGGCRTLPL
                     QYGVRRAAWMISPSFVVPFLMINAGAWAGVLTGNFWLLQLLGASMAAYGLYVCFLMLR
                     RPEDLAVEENHVSWAHMYRMMFVAQVGFALAYLL"
     gene            complement(24144..26429)
                     /locus_tag="PKK95_00105"
     CDS             complement(24144..26429)
                     /locus_tag="PKK95_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOC16633.1"
                     /translation="MEDLELSANRMAESAQRVVDRAVEESRRREHSLITNEHIFLAFA
                     QVEWDMFSQVMRDLDLNPHEILQALEEHLRMVPAFGDADTRVAPATKLLFKLAFHHAN
                     RAGRQQVEAVDLFAAIFEESQGVPVSIIRRHGVEPELLVSRIATRMRDLEQREERLRK
                     KFELPPFLKHFATNLNYYARQDKIPPLYGRTEEMQQVMEILCHRERANSVMLIGEPGV
                     GKTAIVEGLARRIEFEPESVPVRLRDVQVVNLQMNSMVAGTMLRGMFEDRIQNVIREI
                     KEHPNLILFVDEVHTMIGAGSALGAPSDAANVFKSVLARGEVRMIGATTLSEYKEYIQ
                     EDEALARRFRTVHVKEPDLHETRRILFNLRPRLERNYSVKVLDEAIDTAIEMSPRYMR
                     HLHLPDKVIGWLDTASVRAEIARKWEVKAPDVVSVISGVAGIPEDMVFRDVTDRFKEI
                     ESRLSERLIGQRDAIRAVANRLVLNKGPLKDGFDRPDGVLLFLGPTGVGKTELAKAVA
                     EFLFGDERKMIRVDMSEYQDGMVAVDKLIGMPRGIVGSERGGILTNQLRDNPYTVVLL
                     DEVEKAAPNLLTLFLQAFDEGWLTDGRGKRVYLSDAIVIMTSNLGSEHFRKLTNPLGF
                     LSREVGVDQVRGEVMRELERRFPPEFRNRVDEVVLFSPLSRVEVTRIAENYIAAVART
                     LERSGRRLVLGPGTLERIIDEGHSLAFGARFLKRVIDDRIKLPISQRWHDGEVFYALL
                     RDGEVVVDVGAGAEGMKLAIG"
     gene            complement(26684..27520)
                     /gene="htpX"
                     /locus_tag="PKK95_00110"
     CDS             complement(26684..27520)
                     /gene="htpX"
                     /locus_tag="PKK95_00110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOC16634.1"
                     /translation="MNNGIKTALLLGLLSGLLLVIGQLLGGQSGLVIAFGFAVVMNFA
                     SYWFSDKIVLMMYKAQPVGPEHPLARVVDRLAQRGGLPMPKVYVIPDLSPNAFATGRN
                     PQHAAVAATEGLLRILDERELEGVIAHELAHVKHRDILIGSIAATIAAAIMMLARMAQ
                     FAAFFGGAGRDDDRGSNPIALLATIILAPIAAMLIQAAISRSREFAADRGGAAIAGNP
                     YGLVDALRKIETAARRVPLDANPATAHMFIVKPFSAKGLLSLFSTHPPTEQRIRALLG
                     TA"
     gene            27667..28227
                     /locus_tag="PKK95_00115"
     CDS             27667..28227
                     /locus_tag="PKK95_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16635.1"
                     /translation="MRRFLTLGFTLLFTALPVSWSAAQQPPAPRPFPRPPQQQAPAEP
                     AKPVQTPPAAPLPAAEPSEATLGVPVYPTAQFLVSYDAGQGQRYYLFGTTISFAEVVA
                     YYRSVLKQRGDLVFEEPATHMFSIGRFREETMAFPPSVTVKDYTWGGMGGYLNPKLGG
                     EPASFPTIIQVVPPPAGTPAGQRRQP"
     gene            complement(28240..28545)
                     /locus_tag="PKK95_00120"
     CDS             complement(28240..28545)
                     /locus_tag="PKK95_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16636.1"
                     /translation="MTKESLAMLEAAAARVEQAIDVLEEAQEILMEDDEEMFEFPVGI
                     IFSAQTSLRVVSELVRDVKADARKGQRKTKRGAGARKAPAKKTVSKKKTGGKAGPRR"
     assembly_gap    28779..28878
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28933..30999)
                     /locus_tag="PKK95_00125"
     CDS             complement(28933..30999)
                     /locus_tag="PKK95_00125"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOC16637.1"
                     /translation="MIGSLLRDNAVAAALQAGILVLAAAPLPRLLGVWSPRARLAFWR
                     IVMVASVALPLLQPRVALDTAGGAPAIAKPEVVAAADVAGGPHEAVAAPPAASLLSRF
                     SLGGVIAAGALLRLTWLGLGLLVLSRLRRRAVHLWPRSRAVDEAVEAAGTDAEFLVSP
                     FALRPMTFGFARPVILVPSDFPSFPHDQQRAIACHELLHVRRFDWLRAAGDEIVRALA
                     WFHPAAWWLTNQIHLSREQLVDEQVVRRLGNRRPYLEALLQLASTDRRGGLLPASLFL
                     GRAHLPQRVALLVKEVRMSRPRLALSFAAMGIVLALCGGVATAAFPLHRPAADAVLQP
                     QPPAGKADVLRKVYDVRPEFQTVRRAQPLPWLFELVIDASGAVVGVKSLVPGSDPDAD
                     AAIAAMRRWRYEPPRLAPRSVLVGFNLAAGRRDPRQPPILVGGTVKPPKRMVNAAASY
                     PQEAIDAGIEGVVILNVRIDSEGAVSQAEVVRSIPALDRAALDSVLRWRYEPAGFPVE
                     LTATINFTLADSKRAAARGGVAGGVKGGVKGGVAGGVGGGVGGGVGSGVGGGVGGGVG
                     GGVGRGVDGGTGAGVGTGAGAGSGTNYVRVGQETGVEPPRKVVDVGPVYPPAAKEQGI
                     QGVVIIDAHIEPDGRVGHAKVVRSVPGLDEAALDAVRQWKFTPTLKDGTPVGVVMTVT
                     VNFLLQ"
     gene            complement(30996..31373)
                     /locus_tag="PKK95_00130"
     CDS             complement(30996..31373)
                     /locus_tag="PKK95_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOC16638.1"
                     /translation="MRPKSPTLTPQELEIMKIVWGKGEATVREVYETLLARRRIAYTT
                     VMTMMRVLERKGYLKTSREERAYLYRPTRPERQVLRSMVREFVDRVFNGSARPLLVHL
                     VEDRRLSKRELDELQRLVREVEK"
     gene            31521..32048
                     /locus_tag="PKK95_00135"
     CDS             31521..32048
                     /locus_tag="PKK95_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC16639.1"
                     /translation="MMTVSALARRCGLSRSTLLYYEAHGLLRRPQRTPGNYRAYAEAD
                     LRRLEQIGRYRKLGLAVPEIAALLGGKDEGARAILERRLAAIDAEIEALRGHQRAILG
                     LLQRTRRLRRDEMITKDKWVEIMRAAGLTEADMHRWHTEFEKAAPAEHQEFLEFLQIP
                     KDEITKIRDFSRKGA"
     gene            complement(32105..33268)
                     /locus_tag="PKK95_00140"
     CDS             complement(32105..33268)
                     /locus_tag="PKK95_00140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOC16640.1"
                     /translation="MEPRAVVTPRGEDRIAGGHLWVYRSDVADVQASGGEVVAVVGAR
                     RRLIGRALYSDRSQIALRLLVHGDRPVDAAFWRARLEAAIRFREGLGIDATAYRLVHG
                     EGDLLPSLIVDRYGEHLVVQSLSQGIERALPEITAALVEHLAPAGILARNDVKVRALE
                     GLGQEVALLHGAVPPAIPVREGPIEYEVDPWTGQKTGLFLDQRENRAAGASYARGRLF
                     DGFSYNGGFAMRLAASCDQVVAADISADAVARIEANVRRNGLTNVRAIEANVFDELRR
                     LERERERFDTIVLDPPAFAKNKASIERALAGYREINLRALRILSPGGCLITCSCSYHV
                     DEAAFAQVIYEAAIDAGVHVGVVEKRMQGRDHPVLLGVPETYYLKCLIVRRIE"
     gene            33342..33791
                     /gene="rpiB"
                     /locus_tag="PKK95_00145"
     CDS             33342..33791
                     /gene="rpiB"
                     /locus_tag="PKK95_00145"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOC16641.1"
                     /translation="MRIAIGSDHAGYHLKEALKQTLNGAGVEVEDVGTENEESVDYPD
                     YAERVAARVASGQSDRGILICGTGIGMAMAANKVDGIRAASVTDEIGARLARQHNDAN
                     VLALGGRVTPPEVAARLVRIFLDTPFEGGRHQRRVDKVSRLERRQSG"
     gene            33795..>34071
                     /locus_tag="PKK95_00150"
     CDS             33795..>34071
                     /locus_tag="PKK95_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC16642.1"
                     /translation="MSGRHGPATLEAAVDAAFAPGGPLSRTVAEYEPRPGQRAMAGAV
                     ARAIEQGRVLLAEAGTGTGKTLAYLVPSLLSGRRALISTGTKTLQEQI"
BASE COUNT         5167 a        12079 c        11915 g         4810 t
ORIGIN      
        1 agcaggaacg gaggattcag gatcgagagc ttgtcgacat tgtcccgaag gccattcgtc
       61 aacgccagga tgatggagaa gatcatggtg gtggcgccca ccaccgccgt gccgatcagc
      121 accggcttgg ccgtcgcctt gaacgtgttg ccggcgccgt cgcccgtctc gagcaggtcc
      181 ttggccgccc cgaagtcggc gtcgatgccg tggtcgcggc gcagctcctg cttgatgccg
      241 gggatctgct cgatcaccga cagctcgaac accgactgcg cgttgtcggt gaccgggccg
      301 tacgagtcga ccgcgatggt caccgggccc atcccgagga acccgaaggc gaccaggccg
      361 aaggcgaaca ccgccggcgc catcatcagc ccgccgggga acgtcgtgct gacgacgtag
      421 ccgatcgcca tcagcagcat gatcgcgagg cccagccagt aggcactgaa gttgccggcc
      481 accagccctg acaggatgtc gagcgaggcc ccgccctcgc ggcccgaccg gacgacctcc
      541 ttcacgtgcg ccgactcggt cgaggtgaag atcttcacca gctccgggat gatcgcgccg
      601 gccagcgtgc cgcacgtgat gaccgtggag agcttccacc agagcgagcc gtcgcccgcc
      661 aggtcgggga tcagcaggaa cgacacgatg taggtgatga tcaccgacac gatcgacgtg
      721 atccagacca gcgaggtgag cggcgcctcg aagttcatcc gctcggcgtt gccgtaccgc
      781 gcccgcgcga aggtgttgtt gacgaggtag gagaggccgc tggcgacgat catcatgatg
      841 cgcatcacga agatccagac cagcagctgc acctggacgg tcgcgtcggg aaccgccagc
      901 acgatgaagg tgatgagcgc gacgcccgtc acgccgtagg tctcgaaccc gtcggccgtg
      961 ggccccaccg agtcgccggc gttgtcaccc gtgcagtcgg cgatgacgcc cgggttgcgc
     1021 gcgtcgtcct ccttgatgtt gaagacgatc ttcatcaggt cggagccgat gtccgcgatc
     1081 ttcgtgaaga tcccgccggc gatgcgcagc gcggccgcgc cgagcgactc accgatggcg
     1141 aagccgatga agcaggggcc ggcgtactcg cgcgggatga agagcaggat cgacagcatc
     1201 atgaacagct cgacgctgat cagggccatg ccgatgctca tccccgactt gagcgggatc
     1261 aggtagatcg ggaacgcctt gccgcgcagg ctcgcgaacg ccgtccgcga gttggccagc
     1321 gtgttcaagc ggatgccgaa ccaggcgacg ccgtagctgc ccgcgatgcc gatcacgctg
     1381 aaggccagga tgatgccgac gcggctccac tcgaagttct ggaggaagcc gaaatagacc
     1441 acgatgatga cgccgatgaa cgcctcgagg atcagcagga accggccctg ctgcagcatg
     1501 taggccttgc acgtctcgta gatcagctcc gagatgtcgc gcatcgacgc gtggaccggc
     1561 agcgcccgca gctggcggta gatcaccagc ccgaacagca ggccgcccac gcagacgagc
     1621 agaccgacca tcagcagcgt ccgcccgttg acgcccatga agtcgacggc gccgaggtcg
     1681 ggcagcacga ggttcgcctc gccccccacg tgcgccggct cggtcgcctc cgccgccacc
     1741 tgcggcgacg gcgccgccgc ggccagcgcg gccggggcgg cgaccagcac cagggccacc
     1801 agcgcggccc cgacggaaac cagcgggcgg cgcagccacg ccgcaccccc gcgtcgaatc
     1861 agcgaataca ccgcagcgtc cttcatgtca cctcttgtgg ggccgccgtc aggcgggctg
     1921 gccgagccag cgcgatacag ggggcttcaa agccggggga accgggggca gctcaacctg
     1981 ccgattctat atcggtttgc tccggaacgc cggcgccgcg tgcggccacg cccgcaagcg
     2041 gccgcccggt cagctgcttg ggaaccgctg tcccccccgt cagcacgatc cggagcccct
     2101 cctccaccgt caggtccggc aggacgaccc actcgggagg gtagaagtaa acgtcgccga
     2161 tgtacaggtg gttggtcggc acgaagaccg cgaccagccc caccgtcccc tccccgctcg
     2221 acagctcgaa ctcgcgggtg aggaacccga ggcgccgcac gcccgtgcgg tcgacgacga
     2281 tcaccacccg cttgaacccg gcggcgttgt cgggcgagaa cgcctcgatc agctgcttga
     2341 ccggcgagta gacggtcctg aagagcggca gccgcagcag cgaccgctcc agcccgagca
     2401 ccaggcggtt gcccaggacg ttgctcgccg ccacgcccac cagcagcagg aacagcgcgg
     2461 tcgtcaccag ccccagcccc ggcacgtgcc gcccgagcca gcggtcgtac agcggccgcg
     2521 tgatgccgtc gatgaagtcg aacgcccaga tcagggccgc cacgctgacg atcaggggaa
     2581 cggcgacgaa gaaccccgtg acgaaccgcc ggcgcagcca gagcacgggt cgcacgatcg
     2641 cacctcgctc agcgcacgaa cagccagacc acgacggccc ccgccagcag aagccggtac
     2701 cacgcgaaga tcgccagcga gtgccccgtc aggtagcgga ggaagtactt gaccgtgaag
     2761 tagccgacga cggccgagct cgccattccc acggcgaaca gctgcgcctc gccgcccgag
     2821 agcccgcgct cgagcagcat gaccccctcg cggccggccg ctgccaggat cgccgggatg
     2881 cccaggagga aggcgaagcg cgccgcctcc tcgcgcctga gcccgagcac catcccgacg
     2941 acgatcgtcg cccccgaccg cgagacgccc ggcaccagcg ccgaggcctg cgccacgccg
     3001 atccccagcg cctcggcggc ggtcagggtg ccgtgcgcgc gcgtccggcg gccgagcgcc
     3061 tcgaccgcga agaaggagac cgcgacgacc ccgagcgtca ccgccgcgac caccggcgtt
     3121 cgcaggcggt cctcaataac gtcaccgaac aggagccccg cgagaaccgc ggggatggtc
     3181 cccacgacga tgagccgggc gaggcgcgcc gggggggacg ggtggcggtc gaacagccct
     3241 ggcagcgccc gggccatccc gacgaggtcg cgccagaagt agaccaccac cgcgagcagc
     3301 gtgccgacgt ggcaggcgac gtcgaaggcc aggcccatcc gcccggcgtc ccacccgaag
     3361 aacgcgcgcg cgaggatcag gtgcgcggag ctcgacaccg ggaggaactc ggtcagcccc
     3421 tgcaccaccc cgagcagcgc cgccaggacg ctccccacgg cggccggcta cgaggcgcgg
     3481 gcgccgcggc ccgccttgcg ctgccgcgcg gtggtggcgg ccttcgccgc cgctggggcc
     3541 gacgccgcga tgcgggcgcc gcccttcccg tgcggccaga aggtgacgat cgccgccgcg
     3601 atgtagatgc tcgagtaggt gccgacgatc atgccgacga ccatcgtgaa ggcgaacccc
     3661 ttcagcacct cgccgccgaa gaagtacaag aagacgcacg cgaacagcgc cgtgccgccc
     3721 gtgatgatcg tcctgttcaa ggtctggttg atcgagctgt tcaccagatg cgtgatgtcg
     3781 tcgcggcgcg tcccgcgcag gttctcgcgc acccggtcga agatgacgat cgtgtcgttg
     3841 gtcgagtagc cggtgatcgt cagcagcgcg gcgatgacgt tgagcgagat gtcgaacccg
     3901 aagaacacga ggaacgccag cgtcaccagc aggtcgtgca gcgtcgcgat ggtcgccgcc
     3961 gtggcgaagc tgagctggaa gcggaaccag atgtagagca ggatgaagaa gagcgacagg
     4021 ctgaaggcca gcaggccctt ccgggtgagg tcgcggccga cgatcggccc caccacctcg
     4081 gtgctcgaca cctggaagcc gccgacgttc gcggcccgca gcgcctcctc gatctgctgg
     4141 gccggccggc tcagctcggc tccctcctcc cgccccacgt gcggcacgcg gatcatgatg
     4201 cggcgctccg acgcggcgcc gtagctctgg acgaccaggt cctggccccc gccgaaccgc
     4261 ccgccgaccg cctcgcgcac gtcgtcgatg ctcggcaccg tgtcgaactg cacgatgacg
     4321 atggtcccgc ccgagaactc cacgccgagc ggcaggccgc gcaggaagag cgccgcgacc
     4381 ccggcgagga tcaggacggt cgagaggatg atcgcgggcc agcgatacct gacgaagttg
     4441 taattgggat cgtggaagat ctgcatgact agatgctcaa cgactcaacc tgccgcttgc
     4501 tcaggatcgc ttcgaacagc gtcttcgaga cgaaggtcga ggtgaagagg ttcgagacga
     4561 gcccgaagaa gagcgttgtc gcgtacccgc ggatcggccc ggtgccgaac tggaacagga
     4621 acgccgcgcc gatcagggcc gagaggtgcg tgtcgaccag cgtgaggaag accctgctga
     4681 agccggcgtt gatcgaggcc cgcaccccgc gctgggccgc cagctcctcc ttgatgcgct
     4741 cgaagatgag cacgttcgag tcgaccccca tcgtcatcgt caggatgaaa cccgcgatgc
     4801 cgggcaaggt catcgtcgcg cccacgtagg acatcgcgcc gagcaggatg atcaggttga
     4861 acagcagcgc gacgagggcg ttgatccccg acagccggta gtagaacagc atgaacagcg
     4921 cgacgaccac gaggctcagc gacgaggcca cgatgccagc cttgatcgag tcggcgccga
     4981 gcgacggccc gatcgtccgc tcctcgaggt aggtcagcga cgccggcagg gcgcccgacc
     5041 ggaggacgag cgagaggtcc tgggcctcct gctgcgtgaa gccgccggtg atccgcccct
     5101 cgtcggtgat ccgctcgtcg atccgcggcg ccgagcgcac ctggttgtcg aggacgatcg
     5161 cgagctggcg cccgacgttc tcgccggtgg cccgtccgaa cttgcgcgcc ccctcgggat
     5221 tcagcgaaaa gctcacggcc ggctggttgt actcgtcgag cgtcggcttg gcgttcctga
     5281 ggtcgcgccc ggtgacgatc ggcgccttct cgagcaggta gtagaccgtc tcggcgcggc
     5341 cgtcggtccc ggcctgcacg cccggcagca cctcggcgtg cggcggcacc tgcccgccgg
     5401 tcgcggccag cagcccctcc ttggtcgagg ccggtccctg gatgacgagc ttcagctcga
     5461 gcatcgcggt cgagcggatg atctccttcg cccgggcgac gtcgctgatg ccgggcagct
     5521 gcaccagcag ctgctcgccc tccgtcccgc cgtggcgcgc cacgaccggc tcggccacgc
     5581 ccaggtcgtt gacccggcgc tcgatcgtgt ccatcgcctg ccggaccgac tcgtcgcgca
     5641 gctggttctg gcgggccggc cgcatgcgaa acacgtagcc gccgccgacg ccgctctcgc
     5701 ggtcccagcc ggtggccagc gccgtggccg cctggcgcag gaccgcgtcc tgccccggct
     5761 gcagcccgtt cacgcggaac tcggtcggcg aggtcgcctc gacggtcgcg gaggtcagcc
     5821 ccgacttctc gagctcgttc cggaggctct cggcggtcgt ctcggtgtcg agccgcagcg
     5881 cgtcgtcggt gttcacctgg aggaccaggt ggacgccgcc cttgaggtcg aggcccagcc
     5941 ggatcttctg ctccggcggg atgatcgccc agaggcaggc cccgacgatc acgacgatga
     6001 gaaggatgcg ccagcgaagg ttgttcatgc ttgggagtcg tcggccacga ccgggtcctg
     6061 tccctgatac ccggcgatcg cggccctgga aaactcgatc tgcaccttgt cggccacctg
     6121 caccttcacc gtgcggtcgc ccaggcgcgt gatcgtgccg tggatgcccg ccgtggtgat
     6181 gacgcggtca ccgaccttga gcgcttcctg gaaggtctgc accttcttct gccggcgctt
     6241 catcggcaga aggatgatga agtagaagat ggcgaggacc aggaggaacg ggacgaactg
     6301 gacccaggga ctgaccccgg agtcggccgg ggcggccatc gccacgagcg tgcaggcgtg
     6361 caaaaccata ggtgtcatga gtccgcaacg cggggggagt agacctgccg gacgtcctgt
     6421 cgcagacgat cgaacgagcc aaacactaaa gcctctctga tccggcgcat cgtgtcaagg
     6481 taaaaccata ggttgtgcaa cgtgttaagg gtggaggccc cgatctcgcc ggccaggtgg
     6541 aggtggcgca ggtacgcccg cgagaagtgc cggcaggcgt agcagccgca cccctcctcg
     6601 atgggcgccg ggtcgtcggc gaaggcggcg ttcttgatat tgaggcggcc gctcgaggtg
     6661 aagagctggc cgtttcgggc gttgcgcgtc ggcagcacgc agtcgaacag gtcgatgccc
     6721 cgggccaccc cctccaccag gtcgaggggc gtgccggtgc ccatgaggta gcgcgggcgg
     6781 tcagcgggca gcagcggcgc cgtgcaggcc accacgtcgt acatctgccc ggccggctcg
     6841 ccgacgctga ggccgccgat cgcgtacgcc tcgaacccga ccgcgacggt cgcctcggcg
     6901 ctgactcgcc ggagatcggg gaagacgcct ccctgcacga tgccgaactg ggcctggccc
     6961 gggttggtca tctccccggc ggcgccggac gacagctcga ggaagcgcgc gcgggcgcgg
     7021 gcggcccagc gggcggtgcg ctccatcgag gcggccgcgt ggtcgcgctc ggccgggaac
     7081 gccaggcact cgtcgagcac catggcaatg tcggagccca ggcgcgcctg gatgtcggcg
     7141 gcgccttcgg gagtcagttg gtgggggctg ccgtcgaggt gcgatcggaa gcggacgccc
     7201 tcttcgtcga tcgtgcggcg gtcgccgagc gagaacgcct ggaacccgcc gctgtcggtg
     7261 aggaccgcac ggtcccagcc gctgaaggcg tgcagcccgc cgagccgcgc gatgcgcgcg
     7321 tccccgggac gcaggctcag gtggtacgta ttggcgagca ggatctcggc gcccatctcg
     7381 tggaggtcgc gcggaagcac gcccttgacg gccccctggg tgccgaccgg catgaaggca
     7441 ggcgtctgca ccggcccgtg cgcggtgacc atctcaccgc gacgggcctc cccgcaggtg
     7501 tgcgtcaggc ggaaggtgaa gaccggggcc attgagcgta tagtactcgg gctgcggccc
     7561 gattccccag aggcacccct cctgtgaaga agcttcgcat cggtgtgatc tacggcggcc
     7621 gctcgagcga gcacgaggtg tcgctcgcct cggcggcgtc cgtcatcgca aacctcgatc
     7681 gctcgcgcta cgacccggtg ccgatccgga tcgaccgcga cggccgctgg acgattgccg
     7741 accggccgcc cacgacgatt gccgcctcgg ccgtgatcgc caaggcgcgg caggagccgc
     7801 cgcgcagcac gcgcacgggc cgcgaggcct acctcgtccc gcatcccggc gaggagacgg
     7861 tgctgacgat cgagcggaac gcgggcgagg cgaccaacgg ccgggaaaac gcgtacgtcg
     7921 ccggcctcgg cctcgacgtg gtcttcccgg tgctgcacgg cccctacggt gaggatggaa
     7981 cggtccaggg gctgctcgag ctggcgaacg tgccgtacgt cgggtcggga gtcctggcct
     8041 ccgccgtcgg catggacaag gcgatgatga aggtgtgctt cgcggcccgc ggcctgccga
     8101 tcgtgaaaca cctggtcgcc tggcgcgacg agtgggcgcg cgagccggac gcgatcgcct
     8161 cgcgtgtcgg ggcggagctc cgcccgccgg tcttcgtgaa gccggccaac ctcgggtcga
     8221 gcgtggggat ctccaaggcc ggcgaccgcg cggcgctcgg ccgggcgatc gagctggcgg
     8281 cccagtacga ccggaagatc gtgatcgagg aggcggtgcc ggcggcgcgc gagatcgagt
     8341 gctcggtggt gggcaacgcg cggccggagg cgtcggtccc cggggagatc ctgccctcgc
     8401 gcgagttcta cgactacgag gcgaagtacc tcgaagactc gccgacgctg gtgcctgcac
     8461 cgctcgacga ggcggagacc gccagggtcc gccagctggc ggtcgaggcg ttcctcgcgg
     8521 tcgaagcgtc cgggtacgcg cgggtggacc tgctggcatc gcgcgagacc ggcgacatct
     8581 tcgtcaacga gatcaacacc atccccggct tcaccaccat cagcatgttc ccgaagctct
     8641 gggaggcgac ggggctgccg taccccgccc tgctggaccg tctcgtcgag ctggcgttcg
     8701 agcggcacgc ggcgaagcag gcgctgcgca cgggccggcc gtgaagcggc gcgcggccgc
     8761 cgcgctcgtc gtcgtcgtgg cggcgctggg ggccggtccg gccagggcag ccgaaccctc
     8821 gtatttcccg ctgttgggcc gggcgtacga cgcggtgctc gacgcgcggt tcgacgaggt
     8881 ggagccggcg ctcgtcgagg cgtgcgggcc cgcgccggag gtcgcgtgcg acctggtccg
     8941 ggcggtcgcc gtgtggtggc gcatccagct cgacccgctc gatcgctccc gcgacgccga
     9001 gttccagcgc cgggtggacg cggtgatcga ggccgctgaa cagtggtcgc ggcgcgagcc
     9061 gaagagcgcg gaggcgtact tcttcctggg ggcggcgtgc ggggcccgcg tgcagtggcg
     9121 agtgctccgc ggggagcggt tggccgcggc ccgcgacggc aagcggatca aggagagcct
     9181 cgagcgggcg ctcgcgctcg accccacgct cgaggacgcg tacttcggga tcggcatgta
     9241 ccgctactac gccgatctcg tcccgacggt cctcaagttc ttccggtggc tgctgttcct
     9301 gccgggcggc gatcgcgagc ggggactggc ggagatgcag caggcccgcg agcagggcac
     9361 gctgctccgc ggcgaaaccg acttccagct gcacctcgtc tacatctggt acgaggaccg
     9421 ggtggacgag gcgcttgcgc tgctcgccga cctgaggcgg cgctaccccc acaacccgct
     9481 cttcctgcag gccgccgcgg agattcacga cgtctaccgg cacgacgccc ccgccagcct
     9541 cgacgcctgg cggaggctgc tcaacctggc gcgggcgcgg cgcgtctcgc tgccggagga
     9601 gagcgaggcg cgtgcgcggg tcggcatggc ggaggcgctc gacgacctgt accagaccga
     9661 ctacgcactc gagcagctgc gcatcgtgac cgaatcctcg cctgcttcgc cgtacgggct
     9721 cgcggcgcgg gcctggctgc tgctcggcga ggcacacgac cggctgggcg ggcgggaggc
     9781 cgcggtggcg gcgtaccggg cggcgatggc cgcggcgccg cgcgacgatc ccgaccgcgt
     9841 gaagtcgcgc gccagcgagg cgctgcggcg gaggcccgac ccgcgcgcgg ccgaggccta
     9901 tcgccgctcc ctcgaggggt ggcgccacct gcagcgcggc gaactggatc ccgccgccga
     9961 ggcgctctcg cgcgccgccg ccctgaaccc cgccgacccg gtgctgcgct accggctggg
    10021 gcggctgcac ctcgcgctgc accagcgcga cgaggcgctc gcgcagttcg agcgcgcgat
    10081 cgccgctcgc ccggcggcgc cccccgcgct gctcgccgcg gcgctgctcg aggccgcgcg
    10141 gctgctcgag tcgcgcggcg acggcgagcg cgccctctcc ctatacaagc gcgccgccgc
    10201 gctgcgcgga gccgagcccg acacgcggcg cgccgccgcg cgagccgtgt cgcggttgca
    10261 gtgaggcggg attcgggatt caggattcag ggatcgggat tcggaaacgt cgcagcccgc
    10321 cgaacgcgat cagcaccgcg aaacacgatc gcgatccccg cgatcgcccg ccgccgcgcg
    10381 cctctcgtgc gcgatcgcct ccccggatcg caactttttt tgacaacggc actcactttg
    10441 tgcttgacac gtttcttttc tcaaccataa tctacatctc gtatgagcga ggatggctta
    10501 ggccacgcct tgtgatgagc cttcgccggg cccaaggctg ggttttgagg ggtccgggcg
    10561 gaaatagggc gaagtcgccg accgaattgc ctcgcccacc ctggaacgcg ggcgagccac
    10621 ggagcgcttg agcaagggcg gcacgaacgc cgcctgaatc gctcgcgcaa ttccacggat
    10681 gaaggggggg atcaggtaat ggcgaagaag accgcgaaga aggctgcgac caagaaggcc
    10741 ggcaagaagc ggtaagcctc ggcttccgcg agaagagggg gcgggtgtcg cccccttttt
    10801 tattgtacgc gcgtcgcgga agccgccggc ggcgtcgcgg gctattcgaa cgtctgccca
    10861 tcccacacgc gcacgcgcac gatctcgtcc cacggctgca ggcggtccac cacctccatc
    10921 ccggcaacga cgcgggcgaa cacggtgtag cgcccctcga ggtgcggctg cggcgagtgc
    10981 gtgatgaagt attggctgcc cccggtgtcg gggccgtcga gcgccatgcc caccgtcccc
    11041 cgcccgtacg cccgctcgtt cagctcgtcg cggatcgtgt agcccgggcc gccctcgccg
    11101 tccccgcgcg ggtcgccgtc ctgcacgacg aagtccggca ccacccggtg gaagagcaac
    11161 ccgttgaagt acccgcgcct ggcgagcgcg gcgaagttcg cgcacgtcat cggcgcgtcc
    11221 accacgagca gctccagctg caccacgccc cgcgccgtct cgaggtagac ctgcggcgag
    11281 acgctcggcg acaccagctc gggcgcctcg taccacgagt cgggccggcc cgtcggcacc
    11341 gggcgggcgg gcaccgccgc cgcggcggga tcgagcgacc gcagcagcgc ggcggccttc
    11401 agcctcaccg gccactcgcg gtcggcgagc gcctcctcga ggacggcccg cgcgcgctcg
    11461 ccgtacttcg cgagcgcctc cagcgcggcg gtgcgcgcga tgtacgtgtc gtcgccggcc
    11521 cacgatcggt acgcttccgc caggcggccg tcgcccgacg ccggcttgag ctcaccgagc
    11581 gcggcggccg ccgtcatccg gaccaccacg tcctggtggc cgagcgcctc ggtcacccgc
    11641 gcctcgatgc ccggcgcccg cagctggacg agcgcacgga gcacggcggg ggcgacgcgc
    11701 gcgtcggggt cggccagcat cgccgtcagg cgggggacgg cacggtcggc agccagcgtg
    11761 ccgagcacag tcgccgtcgc cgcccgcacg ctccagtggc ggtcggcgtc gagcccggag
    11821 agcacgatgg tgaagctctc ctcgtcgatc tcgcgaagcg cgcggagcgc ggcggcccgc
    11881 acggcgggcg aggggtggcc ggtcaggtcc tgcacgcgct cctgcgcctc gcgggcgcgc
    11941 gcggccccgg cggcggcaat cgcctcgacg agcgccaccg ggtcgaggtc gcgcgtcgac
    12001 agcaggcgcg cgagcgtctc gcgcgccgaa ggatcgccta cctgccccag cgcgcggatg
    12061 gccgacaccg cggcgcgcgg gtcgtcgcgc cagagatcgg ccatcgccgt cagcgcggga
    12121 gacgcggccg ggtccttcag gctgcccagc ccgcgcgccg cgaacgatcg gccgaagctg
    12181 ccgccggcgc gcgcaaaggc gagcagcgcc ggcagcgcgc gccgatcgcc ggtgcgctga
    12241 aacgcgaagg cgaccggcca ccagccgacg cgcggcttgc cgcctggatc gagcaccgcg
    12301 gcggcgagcg gctcgtacgc cttcaggcag ccgagcgcca ccacaccgag ccggaacgcg
    12361 tcggccgccg gctcttgccc ctcgggggga gcgtccggct cgagcgccgc cacgttggat
    12421 gactcgagct ggcgggcgac catgcgcccg atgtccaccg ccgcgtcggc ctggccgagc
    12481 aggctcagcg cctcggccgc gcggccctgc acccgcggat cggggtcgtc gagcgcgcgg
    12541 cggaggggcg ttgcgccgcc cgcgtccccg agcacgccga gcgcgaacgc tgctgcctgc
    12601 cgcaccgccg ggtcgggatc ggattcgagg gtggccgaaa gcaccggcac gccctcgggc
    12661 agccccaccc gcccgatggc gagcgcggcg ctgcgccgca cgcgcgcaag gtcgtcccgg
    12721 acgagcgcga gcaggtcggg cgagggcggc gggggcggca gggtacgccc tcgcctcggg
    12781 gcgatggccg gcgccggcgc cggatcgcgg agcgcccgct gatcctcgag ccgcaggatc
    12841 cacgagagct tctgttccat cgcgatcacg ggagcgggcg gaggcgcggc ggtcgcgcac
    12901 cccgcgagca gcgcgggaag gacgacgacg agacgcgcgg cgagcgccgg gcgaaggcac
    12961 gcgggtgtgc ggagatggca ggtcatcgcg tgctcggatc ttatcacccg gcttcggcac
    13021 gccgggcagg ccccggctca ccggctcgcc gccggcgggc tggcccccga tgtatactgc
    13081 ctcgtcttgg atttccgctc tatcgccttc gaggggccgc ccggcgtcgg caagaccgcg
    13141 ctcgccgagc gggtcgccgc gcgcctcgac gcgaccaccg tgctggagga gaccgagaac
    13201 ccgttcctcg ccgacttcta tggggaccgg ccgggcgcgg ggctgcaggc ccaacttttc
    13261 ttcctgctgg gccgccaccg ccagcaggtc gcgctgcgcc aggccgacct gttcagccag
    13321 atcaccgtct gcgactacct cttcgagaag gaccgcattt acgcgttcct caacctcgac
    13381 gacaacgagc tgttcatcta ccagcggctg tacgacctgc tcgcccgcga cgtcgcgccg
    13441 cccgacctgg tcgtctacct gcaggcgccg accgacttgc tcctgaagcg gctgcgcgcg
    13501 cgggacgagc agcagccgta tcgcctcaac ccggagtaca tcaagaagct gatcgaggcc
    13561 taccagcact tcttctttca ctactccgcc acgccgctgc tggtcgtcga gacctcgcag
    13621 ctcgacctcg gcggcagcga cgaggcgctc gacgacctcc tgcgccagat ccggggcatg
    13681 gggcgcggca cgcggtacta cgtgccgcga acgcgggact gagctgactg aagcgccggg
    13741 gcgcgtgagt tgatatggcc tggttcaaga agacgcggaa gccgatcgat cctgccgtga
    13801 aggccagccg ggtacccgag ggcctgtacg tgaagtgccc cggctgctcg gagctgctct
    13861 acaacaagga cctcgcggcc aacctctcgg tctgcgggaa gtgcggccac cacttccgga
    13921 tgaccgcggc ggaacgtctg gcgatgctct tcgacggccc ctggaccgag cacgacgccg
    13981 gcctcgtctc caccgacccg ctcggcttca aggacaccaa gtcgtaccag gatcgcctgc
    14041 gggccgccgc cgccgcgacg ggggtgaaag aggcggtggt gaccgccacc ggccagctcg
    14101 acggacggcc cgccgtcatc gccgccatgg agtacggctt catcggcggc agcatgggcg
    14161 tggtggtcgg cgagaagatc gccagggcca tcgagcgggc gatcgagggc cgcgcggcgt
    14221 tcatcgccgt ctcgtgctcg ggcggcgcgc ggatgatgga gggggcgctc tcgctgatgc
    14281 agatggccaa gacgtcggcg gccctcgcgc gcctcgaccg ggccggcctc cccttcctct
    14341 cgatcctcac cgaccccacg accggcggtg tgacggcgag cttcgcgatg ctcggtgacc
    14401 tgaacatcgc cgagcccaag gccctcatcg gcttcgccgg cccccgcgtc atcgagcaga
    14461 ccatccgtca gaagctgccg gagggcttcc agcgcagcga gttcctgctc gagcacggca
    14521 tggtggacct gattgccgac cgccgcgagc tgaaggccgt gatcgcgcgc gccctccggt
    14581 tcatgctggc atgacctccc cctcggatcc ggtggccgcg ctgctcgcgc tcgagaagct
    14641 cggcatcaag ttcggcctcg acaacatccg cgccatctgc gacgccctcg atcacccgca
    14701 ggacgcgtgg agttctgtgc tggtggcggg caccaacggc aagggctcgg tggcggccat
    14761 ggtcgaggcc ggcctccgcg cctcgggcag gcgcaccggc ctgtacacct cgccgcacct
    14821 gatccgcctc gaggagcggt tcgccatcga cggacggccc gtcgacccgg atcggatgcg
    14881 gtctgccgcc agccgcgtgc tgcgcgccat cgcgtcggcc cgggcccgcg gcgcgctgct
    14941 gaccgaccca accttcttcg aggcgacgac ggcggtcgcc ttcgacctgt tccgcgaggc
    15001 cggcgtggag gtcgcggtgc tggaggtggg actcggcgga cgcttcgacg cgaccaacct
    15061 cgtggtcccc gcggccgggg cgatcacgac gatcgacctc gaccaccagc agctgcttgg
    15121 gcacaccctg gcggccatcg ccgccgagaa ggcgggaatc gtgaagccgg ggatgcgcct
    15181 cgtggtcggc gagcggaagc ccgaggccgt cgacgtgatt gccgcggcgt gcgcgagcca
    15241 aggcgccacg atgatccccg cctgggacgg cgtcgccgcg gcggtcgcca cccgagcggg
    15301 cggcctcgcg tcaatggacg caggcgtcag cctttcgctg cggacaccca ggcacgatta
    15361 cggcgaagtc agcctgtctc tgcggggacg ccaccaggcg gacaacgccg tggtcgcggt
    15421 gcggctgctc gaagcgctcg ggcccgcggg cctgcacgtg cctcccggcg ccgtccaggc
    15481 ggcgctcacc tccacccggt ggccgggccg cctcgacctg cggcggctcg acgggggccg
    15541 ctccgtcctc ctcgacgcgg cgcacaaccc ggctggcgcc ggcgtcctgg cgtcttactt
    15601 gagtgaggtc gcaccgtcgg gcctgccgct ggtcttcgcg atcatgaagg acaaggatgc
    15661 gccgggcacg ctggcgccgc tgctccccta cgcgcgtccg ctcatcgtga cgcgcccctc
    15721 gaccccgagg gcgttggcgc ccgaggaggt cgcccgaatc gcggagcgcc tcgggtaccg
    15781 cgtgccggtc gaagtggagc cggaggttgg cgcggccctc gacgccgcct ggcggcgggc
    15841 ggggtcgatc ggcgcgtgcg gttcgatctt cctcgtgggg gaggcgctcg agcggctcgg
    15901 cctcccggcc tggtgacggg gcggcgaggc ccggacgagg cgaactcctg tggtattctg
    15961 gccgcgttct cacacggatc ctgaccgcta actacgtgtc ccgcatagtc ttgcccctcc
    16021 tcgtcgcacg cccccggcgc gccgcgctcg ccctggcgct cgcgtcggtt ctcgtgtggc
    16081 cggcgggagc ggccgcgcag gcgccccggc ccggcgcaac atcgtcgcgg gagaccggct
    16141 tcgacacctc caagcagtgg cgcatcgagc agccggcaaa gaaccacctc cggctgatcg
    16201 gcgaggtcga ggcccggcgg ggggacgcca ccttcacggc cgacctcatc gagtcgttca
    16261 ccgacaccca cgaggtcgtg gcgacgggca gcccggggcg gccggtggtc ttcacgcagg
    16321 gcggccagtg gatcgcggcc gagcgggtgg agttcaacac cgagacgcag ctcggcacct
    16381 tctacgaggc gtacgggtcg ttcaccgtcg cctccgagcc gaagcggtcg atgcacggcg
    16441 gccaggagcc cgacgtcctc ttcaacggca ggaagatcga gaaggtcggc ccgaagaagt
    16501 accgcatcac cgacggcgcg ttcacgacct gcctgcagcc gaccccgcgg tggaacgtga
    16561 gcgcgggaac gctggtgttg aacctcgacc gctacgcctt tctccgcggg gcggtgcttc
    16621 gggcgaaggg gctgccggtg ctctacctgc cgttcctgta ctacccgatc aacgacgagg
    16681 accgggccac ggggttcctg atgccctcgt acggcacctc cacgtaccgg gggttcaccc
    16741 tcagcaacgc gttcttctgg gcgatcggcc gcagccacga cgcaaccttc ctgcacgact
    16801 ggtacgccaa gcgggggcag gggctgggca ccgagtaccg gtacgtgggg ccgggcgggg
    16861 ccggcggcga cctccgcttc tacacgctgc gcgagcgcga atccacgatc acctcgggcg
    16921 gggtggagtc gctgctgccg aagcggacga gctacgaggt caacgggaac atcaaccagc
    16981 cgcttggtgg ctcgtggacc gcgcgcggca acgtcgacta cttctccgac atcgaggtcc
    17041 agcagaccta ccacacgaac atctacgacg cctcgcgcag ccagcgctcg gtgaacgcgg
    17101 ggctgagcgg cacgctcgcg cagctgacgg tgaacgccaa ctaccgccgc agcgagtatt
    17161 tctcggggcg cagcaacacg acggtggtcg gcggcgcccc ccagatccag atcacgcgcg
    17221 gcgagcagcc gctcttcggc ctgccgctgt acttcggcgt cacgggggag tacgccaagc
    17281 ttcagcgcga gtaccaccgc acgagcggcg tcaccgacag caacctgagc cgcctggacg
    17341 tcacgccgac cctgcggttc ccctttacca agcttcggtg gctgaccgtc aacacctcgc
    17401 tcgccttccg cgagacctgg tggtcgcgaa gccgcgtcgc cgacaccaac gacttcgtgg
    17461 acgacgggat ctcgcggcgc tacgcgcaac tctcggcgcg cctggtcggg ccggtgctga
    17521 accgggtgtt cgacacgccc ggcttcggct acgccgagcg cctcaagcac acggtcgagc
    17581 cctacgtcga catccgccgc gtgacccggg tcgacaactt cgacgagatc atccagaacg
    17641 acgggaccga ctcggtggtg gggggcacca cgagcatcac ctacggggtg aacaaccgcc
    17701 tgctggcgaa gcgtcgcggc ggcgagggcc cctcgtcgcc ccgcgagttc gtcacggtgg
    17761 gtctctcgca gacctactac tccgactccc gggcgagcca gttcgaccgc agctactcga
    17821 ccagctactc ggggctgccg ccgagcaagc gttcgccggt ctcgctcacg gcccgcgtca
    17881 cccccacccg cgaggtgaac gggtcgctcc gaatggagta cgacgaccgg gcgaagaaga
    17941 tccggagcct gtcggcctcc tggcagtacg cggcgtcggg ctggctgaac ctggtcggcg
    18001 gctacagcca gcggcgcgtg atcgactacg ccgagtcgac ggtcaccgag accaagtacc
    18061 gcctcgacaa ctccatcaac ctgtccacga cgttgcgcag cccggcgaac cgggtcggcg
    18121 gcagctacac cttcaactac gacatcgacc gctcgaatct gctgagcagc cgcgtcatcg
    18181 gatactacaa tgcgcagtgc tgcggcttcg ccgtggagta ccagacggtg acgtacggct
    18241 cctcgtcgcc aatcggcaag gacaaccggt tcaatttctc cttcaccctg gcggggctgg
    18301 gcaccttctc caacttcttc ggcgccctcg gcggcggcgg ggtgcgctga gccggcggga
    18361 ggacgatgaa gggactgatc ctcagcggcg ggaagggcac gcgcctccgg ccgctcacct
    18421 acacgagcgc caagcagctg gtgcccgtcg ccaacaagcc cgtgctcttc tacggcatcg
    18481 aggcgatggc cgaggccggc atccgcgagg tggggatcgt ggtcggcgac accgcgcccg
    18541 agatcgaggc cgcggtcgcc gacgggtcgc gctgggggct gcgggtcacc tacatcccgc
    18601 aggatgcgcc ccgcgggctg gcccacgccg tgctcatcag cgagggcttc ctcggcggcg
    18661 agcccttcgt gatgtacctc ggggacaacc tgctgaacaa ggggatcggt cctttcgtgc
    18721 ggcagttcga ggccgagcgc cccgcggcgc agatcctgct cgcgcgcgtc tccgatccgc
    18781 agatgttcgg tgtcgccgag ctcgagggcg gcagggtcgt gcggctggtg gagaagccgc
    18841 gcgagccgaa gagcgactac gcgctggtcg gcgtgtacat gttcggcccc gagatcttca
    18901 cctcggtccg gcgcatccgc ccgagctggc gctccgagct ggagatcacc gacgcgatcc
    18961 aggacctgat cgaccgcggc ctcgaggtgc ggccccacgt ggtggacggg tggtggaagg
    19021 acacggggaa gctcgaagac ctgctcgagg cgaaccggtt gatcctcgac acgctggagc
    19081 gccggatcga gggagaggtg gacgaggcgt cgcggatcga gggcaaggtg gtcgtcgagc
    19141 cgggcgccgc gatcgagcgg tcggtggtcc gcgggcccgt cgtcatcggc tcgggggccc
    19201 ggatcgcgca ggcctacatc gggccgttca cctcgatcat gaacgacgtg caggtgcgcg
    19261 acgccgagat cgagcacagc atcgtgctcg agggatccga gatctgcggc ctggcgagcc
    19321 gcatcgagga cagcctgatc ggcaagaacg tccggatcta ccggatgccg gtgaaaccgt
    19381 cggcctaccg cttcatgctg ggcgacaact cggaggtcgg catcaaatgg tgaacgacat
    19441 cacgaaggag cccgcgccct acgtcccgcc gggcaagcgg gcgcgcatcg acggcgtcaa
    19501 gacgaaggcc ctccggatca tcccggacga ccgcggctgg ctgatggaga tcctgcgcgc
    19561 cgacgaggcc gacctcttcg agcgcttcgg gcaggtctac gtctcggcga cctaccccgg
    19621 ggtggtgaag gcctggcact accacaagcg ccagaccgac aacttcgcct gcgtgcaggg
    19681 gatggtgaag ctggtcctgg tggacacgcg gcccggctcc cccaccgagg gggtggtcaa
    19741 cgagttcagc atcggcacgc tcaacccgat gctcgtgcag gtgccgcggc tcgtcttcca
    19801 cggctggaag tgcaccggca ccgagcccgc gctcgtggtc aacgcgccca ccgaaccgta
    19861 ccgatacgac gagcccgacg agtaccgcct cgagccgcac ggcacgctcc cctacgactg
    19921 gagccggcgc gatggttgag gtgctggtga ccggcggcgc ggggttcatc ggcagccagt
    19981 tcgtgcgctc ggccctggcg caccccgatt ggcgcgtgac gaccctcgac aagctgacct
    20041 acgccggccg gctcgagaac ctgcaggagg tgctcgacca cccgcgccac cgcttcgtcc
    20101 ggggcgacgt ctgcgacccc gggctggtcg gccccctcgt cggcagcgcg gagatcgtcg
    20161 tgcacttcgc ggccgagacc catgtcgacc gttcgatcca ggccgccggc gacttcatcc
    20221 gcaccgacgt gtacggcacg ttcgtgctgc tcgaggcggc gcgcacggcg ccccggttgc
    20281 ggcgcttcgt gcagatctcg accgacgagg tctacggcag cgtgccggag ggggcgagtc
    20341 gcgagaccga cgaactgcgc ccgcgcaatc cgtacgcggc gagcaaggcg ggcgccgacc
    20401 gcctcgccta cagctactgg gccacctacc gcgtcccggt cgtcgtgacc cgcgcgtcga
    20461 acaactacgg gccgcgccag tttcccgaga agctgatccc cctcttcgtc accaacgcga
    20521 tggacggcct ccccgtgccg ctctacggcg acggcctcaa cgtgcgcgac tggctgcacg
    20581 tcgaggatca ctgccgcgcc ctcgacctgc tgatcgagcg cggcacgccc ggggaggtct
    20641 acaacatcgg cggcggcaac ctcgtcccga acgtcgacct cacgcgccgc atcctcgcgc
    20701 tcctcggccg gcccgagagt ctcgtccagc cggtggcgga tcgcccgggc cacgaccgcc
    20761 ggtactgcct cgacacctcg aagctgcggg gcctcggctg ggagccgtcc gtcccgttcg
    20821 agggcggcct ggaagccacc gtcgactggt atcgcaccaa cgagtggtgg tggaggccga
    20881 tcaagcaggg tgacccggcc ttccgcacgt attacgagca gcagtacggc cggcgatgaa
    20941 cggcccgatc ctcgtcaccg gtgcggcggg gttcgcgggc agccaccttc tcgacctgct
    21001 cggcgccgcg gacgtccccg tcgtcgcctg gcggcgcccg ggtgaggcgc tgccgccgcc
    21061 gccaacgggc ggcgcgtgcc ggtggatgga ggtggacgtc ctcgaccgcg acaacgtgga
    21121 acacgcggtc cgggaggccg cgccgtcggc ggtctaccat ctggccggcg ccgcccacgt
    21181 gggcacgtcg tgggacgatt cggccgcgac gcttgcggtc aacgtcctgg gcacgtggca
    21241 cctgctcgcc tccctcgcgg cgttgcccgg gcggccgcgc acgctcgtat cgggatcggc
    21301 gctggtctat gctcgccagg accgcgcgat cagcgaggac gaccccgtcg gcccggcgag
    21361 cccctacgcg gtcagcaagc ttgcccagga gctggcggcg gcgcacgccg tgcgcgagct
    21421 gggcctcccg gtcgtcatca cgcgctcgtt caatcacatc gggccccggc agtcgccctc
    21481 gttctcgtcg gccagcttcg cgcgacagat cgcgctcgcc gaggcgggca ggggaccgcg
    21541 ggagctcctg gtgggcaacc tcgacccgcg ccgcgacctc accgacgtgc gggacatcgt
    21601 cgccgcctac cgggcgctgc tcgacgcggg ggcgcccggc cgggtctaca acgtctgctc
    21661 ggggcacgcg gtgcgcgtga gggacgtgct cgacggactc gccgcgctcg cgcgggtgcc
    21721 gatcgacgtc cgcgtggacg aggcgctgct ccggcctcac gacactccgc tggtgctcgg
    21781 caacccccgc cggatccgcg aagagatcgg gtgggagccg cggatcgggc tggccgacac
    21841 cctccgcgac ctgctcgact actggcgcga ggccatcctc cgatgagcgg tttctccgag
    21901 agccggcggc aggccatcca catgtcgatg gccctcttcg cgctggcgct gcgatacctg
    21961 gcctggtggc aggccgcgat ctgtgcggtt gccgccttcc tgttcaacct gctggtgctg
    22021 ccgcgtctcg gcggcagttc cctctaccgg ccttccgacc aggctcgcgg gtaccccctg
    22081 ggcatcctct tctacccgct gtcggtgctg acgctgatcc ttgccttccc gcggcggccc
    22141 gacatcgtcg cggcctcgtg gggaatcctg gccgttggcg acgggctcgc caccatcgtc
    22201 gggcgccgcg cgggacagcg gcgcttgagc tggaatcccg agaagacggt cgcgggcagc
    22261 ctcgcgctcg cccttggcgg cggggcggcg ggagtcgtcc tgaccgcctg gacccgcccg
    22321 gccgtgtcgc ccgagccgcc gctggcgttc gtgctgctgg cgccgttcct ggctggggcg
    22381 gccgcggcct tggtggagtc gctccgcgtc cggttggacg acaacatctc ggtgccgatt
    22441 gcagcggcga tcgtcctcgg ggcgctgggg ttgatggacg cggaatcggc cggcgcggcg
    22501 gccgcctggc tgccacgcgc gttccccctg gcactggcgg tcaacgtgcc ggtggcgctg
    22561 cttggatggc gcgccggcag cgtcagccgc tccggggcaa tcgccggggc ggtcatcgga
    22621 atcctggtac tgaccttcgc ggggccggcc ggatgggcgc tgctcttcgt ctcgttcctc
    22681 ctggcaaccg cctcgacgag gctcgggttg aagcgcaagg ccgtgctcgg gatcgccgag
    22741 gagcggggcg ggcgccgcgg gcccggcaac gcgatcgcca acaccggcct ggcggccctg
    22801 gcggcggcgg tcgcggccct gtcgccctac cgcgacggtg cgctgctcgt gatggtggcc
    22861 gcgctggccg ccggggccag cgacacggtg gcgagcgagg tcgggaaggc ctggggtcgt
    22921 cggacgttcc tcttcccgtc gctgcgtccc gtcaagcccg gcacctcggg cgccgtgtcg
    22981 ctcgagggga cggcggcggg gctggtctcg gcgctggcct tgtcgagcct tgccacggcg
    23041 ctggggctga tcaccggcaa cgcgttgtgg ttcgccgcaa tcggcgccac cttcggggcg
    23101 ttcgtggaga gcggccttgg cgccacgctc gagcccggcg gaatcctcga caacgacatg
    23161 ctgaacttca tcaacaccgg tgccgccggc gcggcggcgg tggcgctgtc atggatctcc
    23221 aggtaaggcc cgtgcgggcc cgcgcgacga cctggctcga gttcgccagg cccttcacgc
    23281 tcttcgcccc cgcgctcggg atggcgtcgg gcggcgtggc ggcaatcggt gcgcagcctc
    23341 ccgagccctg ggcctggcac ctggtggcct acccgctgct cggcgcggcc gcggcggcgg
    23401 tgctgaacgc ggcgtcgaac ggcctgaatc agatctacga cctcgagatc gaccgggtga
    23461 acaagccgcg gcggccgctg cccagcggca ggatgtcggt tggcgaggcg tgggcgttca
    23521 cgatcgcggc tttcgcgatc tcctggctgc tcgcctggac gatcgcgccc ggggggcgcc
    23581 gccacacctt ctggatgttc gtcgcggcca gcctcatcac cgcgctctac tcggtaccgc
    23641 cgttccgcac caagcggctc ggcgtctggg ccaacgtcac ggtggcgatt ccccgcgggg
    23701 cgctgctgaa ggtcgccggc tggtcgacgg tgaagacggc gttcggatgg gagccgtggt
    23761 tcatcggggc gatcttcggc ctgttcctgc tcggggcgac gaccacgaag gatttcgctg
    23821 acatgaaggg cgacgcgctc ggcgggtgcc gcacactgcc cctccagtac ggcgtccgtc
    23881 gggcggcctg gatgatctct ccctcgttcg tcgtcccgtt cctgatgatc aacgcggggg
    23941 cgtgggcggg cgtcctgacg ggaaacttct ggctgctgca gctgctcggc gcctcgatgg
    24001 cggcctacgg cctctacgtc tgcttcctga tgctgcggcg cccagaagac ctggcggtcg
    24061 aagagaacca cgtctcctgg gcgcacatgt accgcatgat gttcgtcgcg caggtcggct
    24121 tcgcgctggc gtacctgctg tagtcacccg atcgcgagct tcatcccctc ggcccccgcg
    24181 cccacgtcca ccacgacctc gccgtcgcga agcagcgcgt agaacacctc gccgtcgtgc
    24241 cacctctggc tgatcggcag cttgatgcgg tcgtcgatga cccgcttcag gaaccgcgcc
    24301 ccgaacgcca ggctgtgccc ttcgtcgatg atgcgctcga gcgttcccgg gccgagcacg
    24361 aggcggcggc ccgaccgctc gagcgtccgc gccacggccg cgatgtagtt ctcggcgatc
    24421 cgggtgacct cgacgcgcga gagcggggag aagagcacga cctcgtcgac ccggttgcgg
    24481 aactcggggg ggaaacggcg ctccagttcc cgcatcacct cgccgcgcac ctggtcgacg
    24541 cccacctccc tcgagagaaa cccgagcggg ttggtgagct tgcggaagtg ctcggagcca
    24601 aggttcgagg tcatgatgac gatcgcgtcg ctcaggtaga cgcgcttgcc gcggccatcg
    24661 gtgagccagc cttcgtcgaa ggcctgcagg aacagcgtga gcaggttcgg cgcggccttc
    24721 tcgacctcgt ccagcagcac gaccgtgtag gggttgtcgc gcagctggtt ggtcaggatg
    24781 ccgccgcgct ctgagccgac gatcccgcgc ggcataccga tgagcttgtc caccgccacc
    24841 atgccgtcct ggtactccga catgtccacg cggatcatct tccgctcgtc gccgaacagg
    24901 aactcggcga ccgccttcgc cagctcggtc ttgcccaccc cggtggggcc gaggaacagg
    24961 aggacgccgt cggggcggtc gaacccgtcc ttgaggggcc ccttgttgag caccagccgg
    25021 ttcgcgaccg cccgaatggc gtcccgctgc ccgatgagac gctctgagag gcgcgactcg
    25081 atctccttga agcggtcggt gacgtcgcgg aacaccatgt cctcggggat gccggccacg
    25141 cccgagatga ccgacaccac gtcgggcgcc ttgacctccc acttgcgcgc gatctcggcc
    25201 cggaccgaag cggtgtcgag ccacccgatc accttgtcgg gcaggtgcag gtggcgcatg
    25261 tagcgcggcg acatctcgat cgcggtgtcg atggcctcgt cgagaacctt gaccgagtag
    25321 ttgcgctcca gccgcggccg caggttgaac aggatgcgcc gcgtctcgtg caggtcgggc
    25381 tccttcacgt gaacggtccg gaaacgccgt gcgagcgcct cgtcctcctg gatgtactcc
    25441 ttgtactcgc tcagcgtggt cgccccgatc atccgcacct cgccgcgcgc gaggaccgac
    25501 ttgaagacgt tggcggcgtc cgagggcgcg ccgagcgccg agcccgcgcc gatcatggtg
    25561 tggacctcgt ccacgaagag gatcaggttg gggtgctcct tgatctcgcg gatcacgttc
    25621 tggatccggt cctcgaacat gccgcggagc atcgtgccgg cgaccatgct gttcatctgc
    25681 aggttgacga cctgcacgtc gcgcagccgc acggggaccg actcgggctc gaattcaatc
    25741 cgccgcgcca gcccctcgac gatggcggtc ttgccgacgc ccggctcgcc gatgagcatc
    25801 accgagttgg cccgctcgcg gtgacagagg atctccatca cctgctgcat ctcctcggtc
    25861 cgtccgtaga gcggcgggat cttgtcctgc cgggcgtagt agttgaggtt ggtggcgaag
    25921 tgcttcagga agggaggcag ctcgaacttc ttgcgcagcc gctcctcccg ctgctcgagg
    25981 tcccgcatgc gggtcgcaat ccgcgacacc agcagctcgg gttccacgcc gtggcgccgg
    26041 atgatcgaga ccggcacccc ctggctctcc tcgaagatcg ccgcgaacag gtcgaccgcc
    26101 tcgacttgct ggcgcccggc gcggttggcg tggtggaacg ccagcttgaa cagcagcttg
    26161 gtcgccgggg cgactcgcgt gtcggcgtcg ccgaacgccg ggaccatccg caggtgctcc
    26221 tcgagcgcct gcaggatctc gtgaggattc agatcgaggt ctcgcatcac ctgcgagaac
    26281 atgtcccatt cgacctgggc gaatgccagg aagatgtgct cgttggtgat cagcgagtgc
    26341 tcgcggcgcc gcgattcctc aacagcccgg tccacgacgc gctgggctga ctccgccatc
    26401 cggttcgccg acagttcaag gtcttccacc gttcatagcc tccgctgcgc tcgttgccga
    26461 tgcacctggc aacagcaaaa cacacgccag cgggagggcc cttcaggatg ggggagacta
    26521 agtctcgaga acggggaccc gggagacgga aaccggaacg tggcacgagc gttccgcctg
    26581 tgagcagtac cggccggaaa cggccgctga cgcaggacgc ccccctcgcg gaggcgtccg
    26641 cctgcgggca atcggcgcgg agaaacgcgc agaacctgtc cggctacgcc gtgccgagaa
    26701 gggcgcgaat gcgctgctcg gtcggcgggt gcgtgctgaa cagcgacagc agccccttcg
    26761 ccgagaacgg cttgacgatg aacatgtgcg ccgtcgccgg gtttgcgtcg agcggcacgc
    26821 ggcgcgccgc cgtctcgatc ttgcggagcg cgtcaaccag gccgtatggg ttgccggcaa
    26881 tcgcggcgcc gccccggtcg gccgcgaact cgcgggagcg cgagatggcc gcctggatca
    26941 gcatcgcggc gatcggggcg aggatgatcg tcgccagcag cgcgatcggg ttgctccccc
    27001 ggtcgtcgtc gcggccggcc ccgccgaaga aggcggcgaa ctgcgccatc cgcgccagca
    27061 tcatgatggc ggccgcaatg gtcgcggcaa tcgagccgat caggatgtcg cgatgcttga
    27121 cgtgcgccag ctcatgcgca atcaccccct cgagctcacg ctcgtcgagg atgcggagca
    27181 ggccctcggt cgcggccacc gcggcgtgct gagggttgcg gccggtggca aaggcgttgg
    27241 gcgacaagtc cgggatgacg tatacctttg gcatcggcaa gccgccccgc tgcgccaggc
    27301 gatcgacaac gcgggcgagc gggtgctctg gaccgaccgg ctgcgccttg tacatcatca
    27361 ggacgatctt gtccgagaac cagtacgatg cgaaattcat caccaccgcg aagccaaagg
    27421 cgatgacgag gccgctctgg ccgcccagca actgaccgat cacgagcaac aggccgctga
    27481 gcaggccgag cagcagagcg gtcttgatgc cgttgttcat gcgcgcactt cctccgttgt
    27541 caccttgagg caaacccgga tccgatctta acgtcccggt cggcgggcgg caaccgatta
    27601 cggccggccc gcgccgggtc ggcgggcttc cgtctccttg tcaccgggtt cataatgagg
    27661 gcgattatgc gccgcttcct gacgctcggt ttcaccctgc tcttcacggc cttgccggtt
    27721 tcgtggtccg cggcccagca gcctccggcc ccccgtccct tcccccggcc gccgcagcag
    27781 caggccccgg ccgagccggc caagccggtc caaacgccgc ccgccgcccc gctgccagcc
    27841 gccgagccca gcgaggcgac cctgggcgtc cccgtctacc cgaccgcaca gttcctggtc
    27901 tcctacgacg ccgggcaggg gcagcggtac tacctgttcg gcaccacgat ctcgttcgcc
    27961 gaggtcgtcg cgtactaccg gtccgtgctg aagcagaggg gcgacctggt gttcgaggag
    28021 ccggccaccc acatgttcag catcgggcgg ttccgcgagg agacgatggc gtttccgccc
    28081 agcgtgacgg tgaaggacta tacctggggt gggatggggg gctacctcaa cccgaagctc
    28141 ggcggcgagc cggccagctt cccgacgatc atccaggtcg ttccgccccc ggccggaacg
    28201 cctgccgggc agcgccgcca gccctgagcc ctggcgggtt cagcggcgcg ggccggcttt
    28261 cccgccggtc ttctttttcg atacggtctt cttcgccggc gcctttcgtg ctccggcccc
    28321 gcgcttcgtc tttcgctggc ctttccgggc gtcggccttg acgtccctca ccagctccga
    28381 cacgacgcgc agcgaggtct gcgcggagaa gatgatcccg acggggaact cgaacatctc
    28441 ctcgtcatct tccatcagga tctcctgcgc ctcctcgagc acgtcgatcg cctgctcgac
    28501 ccgcgcggcc gccgcttcca acatcgccag cgattccttc gtcatcggtg cctccgtatt
    28561 gaacactact gataacacac ctcaggcggg cggaccacat cgggcgcggc gattggtacg
    28621 tgtggcgcgg gcggaagggc gcggagcggg attcgaatcc cgctccacac gcgcgcacgg
    28681 ttacgaggag ggctgatcgc gaacaggcga gacgcctgga gcggaacagg cgagacgcct
    28741 ggagcggaac aggcgagacg cctggagcgg aacaggcgnn nnnnnnnnnn nnnnnnnnnn
    28801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28861 nnnnnnnnnn nnnnnnnnag acgcctggag cggaacgggc gagacgcctg cggaacaggc
    28921 gagacgcctg ttctactgga gcaggaagtt gacggtgacg gtcatgacga cgccgaccgg
    28981 ggtgccatcc ttcagcgtcg gcgtgaattt ccactgtctg accgcgtcga gggccgcctc
    29041 gtcgagcccg ggcaccgacc gcacgacctt tgcgtgcccg acgcgtccgt cgggctcgat
    29101 gtgggcgtcg atgatcacca ccccctggat gccctgctcc ttggcggccg gaggatagac
    29161 ggggcctacg tcaaccacct tcctcggcgg ctcgacgccc gtctcctggc ccacgcgaac
    29221 gtagttggtt ccggagccgg caccggcacc ggtgcccacc ccggcgcccg tcccgccatc
    29281 aactcctcta ccgacaccgc cacccacccc gcctccaact cctccgccga caccgctacc
    29341 taccccacct ccaacccctc caccgacacc gccggccact cctcccttca cgccaccttt
    29401 cacgccgccg gcgacgccgc cgcgcgcggc ggcgcgcttc gaatcggcga gcgtgaagtt
    29461 gatcgtcgcc gtcagctcga ccggaaagcc ggccggctcg tagcgccagc ggagcaccga
    29521 gtcgagggcg gcccggtcga gggccgggat cgatcggacg acctcggcct ggctcacggc
    29581 cccctcggag tcgatccgga cgttgaggat caccacgccc tctatcccgg cgtcaatcgc
    29641 ctcctgcgga tacgacgcgg cggcgttgac cattctcttc ggcggcttga ccgtcccccc
    29701 caccaggatt ggcggctgac gaggatcgcg ccggcccgcg gccaggttga acccgaccag
    29761 cacggacctg ggcgccagcc gcgggggctc gtagcgccac cggcgcatcg ccgcgatcgc
    29821 ggcgtcggcg tcggggtcgg atccgggcac gagcgacttc acgccgacaa ccgcgccgct
    29881 cgcgtcgatc accagctcga acagccaggg cagcggctgg gcgcggcgca cggtctggaa
    29941 ctccggccgg acgtcgtaaa ccttccgcag gacgtcggcc ttgccggcgg ggggctgcgg
    30001 ctggaggacg gcgtccgccg cggggcggtg gagcgggaac gcggcggtgg ccaccccgcc
    30061 gcacagcgcg aggacgatcc ccatcgccgc gaacgacagc gcgagacgcg ggcgcgacat
    30121 acgcacctcc ttcaccagca gcgccacccg ctggggcagg tgggcgcgtc cgaggaacaa
    30181 cgaggccgga agaagccctc cgcgccgatc cgtcgaggcg agctgcagca gcgcctcgag
    30241 gtacggacgg cgattgccca gccgccggac gacctgctcg tcgaccagct gctcgcggga
    30301 aaggtgaatc tggttggtca gccaccaggc ggccgggtgg aaccaggcga gggcccggac
    30361 gatctcatcg cccgcggccc gcagccagtc gaagcgccgc acgtggagca gttcgtgaca
    30421 cgcgatggct ctctgctggt cgtgagggaa gctcggaaag tcggacggca cgaggatgac
    30481 cgggcgcgcg aagccgaacg tcatgggccg gagcgcgaag ggtgagacga ggaactcggc
    30541 gtcggtgcct gccgcttcga ccgcctcgtc caccgcccgg ctgcggggcc agaggtgcac
    30601 cgcccggcgc cgtagccggg agagcacgag caggcccagg ccgagccacg tgaggcgcag
    30661 gagggcgccg gccgcgatga caccgcccag ggagaaccgc gagagcaggc tggcggccgg
    30721 cggcgccgcc actgcctcgt gcgggccgcc ggcaacgtcg gcggcggcga cgacctcggg
    30781 cttcgcgatc gcgggcgcgc cgcccgcggt gtcgagcgcg acgcgcggct gcagcagcgg
    30841 aagcgccacg gaggcgacca tgacgatgcg ccagaaggcg agccgggccc gcggcgacca
    30901 gacgccgagc agccgcggca gcggggcggc ggcgagcacg aggatgccgg cctgcagcgc
    30961 ggcggcgacc gcgttgtcgc ggaggagcga gccgatcatt tctccacctc ccgtaccagg
    31021 cgctggagct cgtcgagctc ccgcttcgac aggcggcgat cctcgaccag gtggacgagc
    31081 agcgggcggg ccgagccgtt gaagacccgg tcgacaaact cgcggaccat cgaccggagc
    31141 acctggcgct cgggccgtgt ggggcggtag aggtaggccc gctcctcgcg gctcgtcttg
    31201 aggtacccct tccgctcgag gacccgcatc atcgtcatga cggtggtgta ggcgatgcgg
    31261 cgccgggcga ggagcgtctc gtagacctcg cggacggtcg cctcgccctt gccccagacg
    31321 atcttcatga tctcgagctc ctggggcgtg agggtcgggc tcttcggtcg catactaaga
    31381 aaatagtact gaggaacacg gatgtcaagt gctagtccac ggagtcagga agtcgcgaag
    31441 tcacgaagtc atcaagtcga ggtcgcgaag tcggggcttg acctgtctag aatagacagg
    31501 tcgagactgg aggcgtggcg atgatgacgg tgtcggcgct ggcccggcgg tgcggcttga
    31561 gccgcagcac gctgctctac tacgaggccc acgggctgct gcgacgcccc cagcgcaccc
    31621 cgggcaacta ccgggcctac gccgaggcgg acctgcgccg gctcgagcag atcggccgct
    31681 accggaagct cgggctggcc gtgcccgaga ttgccgccct cctcggcggg aaggacgagg
    31741 gggcgcgcgc gatcctcgag cgccggctcg cggccatcga cgcggaaatc gaggcgctcc
    31801 gcggccacca gcgggccatc ctcggcctgc tgcaacgcac acgccgcctc aggagagacg
    31861 agatgatcac gaaggacaag tgggtggaga tcatgcgcgc ggccgggctc accgaggcgg
    31921 acatgcaccg ctggcacacc gagttcgaga aggccgcgcc ggccgagcac caggagttcc
    31981 tggagttcct gcagatcccg aaggacgaga tcacgaagat cagggacttc agcaggaagg
    32041 gagcgtaggg gctcacctcg cggttgcccc cgggtcgatg tcggggcggc cgcacgcgcc
    32101 gcccctactc gatccgccgc acgatcaggc acttcaggta gtacgtctcg ggaacgccga
    32161 gcaggaccgg gtgatcgcgg ccctgcatcc gcttctcgac cacccccacg tggacgcccg
    32221 catcgatggc cgcctcgtag atcacctgcg cgaaggcggc ctcgtccacg tggtaggagc
    32281 agctgcaggt gatcaggcac ccgcccggcg aaaggatccg cagggcgcgc aggttgatct
    32341 cgcggtaccc ggccagcgcc cgctcgattg acgccttgtt cttcgcgaag gcgggcgggt
    32401 cgagcacgat cgtgtcgaac cgctcccgtt cccgctcgag gcggcgcagc tcgtcgaaga
    32461 cgttcgcctc gatggcccgg acgttggtca acccgttccg gcggacgttc gcctcgatgc
    32521 gggcaaccgc gtcggccgag atgtcggcgg ccacgacctg gtcgcacgag gcggccagcc
    32581 gcatcgcgaa gccgccgttg tagctgaagc cgtcgaacag ccggccgcgg gcgtaggacg
    32641 cgccggccgc ccggttctcc cgctggtcga gaaacaggcc ggtcttctgc ccggtccagg
    32701 ggtccacctc gtactcaatc ggtccctcgc gcaccgggat cgcgggcggc acggccccgt
    32761 gaagcagggc cacctcctgg ccgagaccct cgagcgcccg caccttcacg tcgttgcgcg
    32821 cgaggatccc cgcgggcgcg aggtgctcga ccagggccgc cgtgatctcg gggagcgccc
    32881 gctcgatccc ctgcgaaagc gactgcacca cgaggtgttc gccgtagcga tccacgatca
    32941 gcgacggcag caggtcgccc tcgccgtgca cgagacggta agcggtcgcg tcgatcccca
    33001 gcccctcgcg gaaccggatg gcggcctcga ggcgggcgcg ccagaaggcg gcgtcgaccg
    33061 gccgatcgcc gtgcaccagc agccgcagcg cgatctggga cctatcgctg tagagggccc
    33121 gcccgatcag gcgccggcgc gccccgacga ccgcgaccac ctcgccgccc gaggcctgga
    33181 cgtcggccac gtcgctccgg tacacccaga ggtggccccc ggcaatccgg tcctcgccgc
    33241 gcggggtgac gacggccctc ggctccatcc ggtcaagctt acccgatgag cggctggagc
    33301 agggccgatc ccggcggcga acctcctgct acaatcgtcc ggtgcgcata gcgatcggct
    33361 ccgatcatgc ggggtaccac ctcaaagagg cgctgaagca gacgctgaac ggcgccggcg
    33421 tcgaggtgga ggacgtcggc acggaaaacg aggagtcggt cgattacccc gactacgcgg
    33481 aacgggtcgc cgcgcgcgtg gcttcggggc agtcggaccg ggggatcctc atctgcggca
    33541 ccgggattgg catggcgatg gcggccaaca aggtcgacgg gatccgtgcc gcctcggtga
    33601 ccgacgagat cggcgcgcgg ctggcgcgcc agcacaacga cgcgaacgtg ctcgccctcg
    33661 gggggcgcgt cacgcctccg gaggtcgccg cgcggctcgt ccggatcttc ctcgacacgc
    33721 cgttcgaggg cgggcgccac cagcggcgcg tggacaaggt ttcgcgcctc gaacggcgac
    33781 agagcggctg agcggtgagc ggcaggcacg gtccggccac gctcgaggcg gccgtcgacg
    33841 ccgcctttgc ccccggcggc cccctgtcgc gaaccgtcgc cgagtacgag cctcgccccg
    33901 ggcagcgggc gatggcaggg gccgtcgcgc gcgccatcga acagggccgg gtcctgctcg
    33961 ccgaggccgg cacgggcacc ggcaagaccc tcgcctacct cgtgccgtcg ctgctcagcg
    34021 gccgccgcgc cctgatctct accggcacca agaccctgca ggagcagatc t
//
LOCUS       DAOPQX010000002        30894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_1912_length_30894_cov_4.985538, whole genome shotgun sequence.
ACCESSION   DAOPQX010000002 DAOPQX010000000
VERSION     DAOPQX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 30894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30894
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1912_length_30894_cov_4.985538"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1008)
                     /locus_tag="PKK95_00155"
     CDS             complement(94..1008)
                     /locus_tag="PKK95_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOC16643.1"
                     /translation="MHAIVPAPQTLRRPVTILKVLAAIALWGGSFVATKIALRELSPL
                     AIIAARFGLGLAFLLALLAWRRQTRLADRGDLAWLALLGVIGVTVHQLLQSVGLLTTT
                     ATNSGWMIALIPVFTAVLARLMLGEPFGRHKVLGLAAASAGALVVAARGFDLRGLVAN
                     PAPGDGLMLLSAANMALFTTWSKRVIGRYPPAVTMTHVLAFGWLASLPLAAVDLGWQA
                     LPSLTAGGWWSLVFLGVGCSGVAYAFWYDALAETDASALASFQYIQPIVTLAVANRVL
                     GEPVTPAVVAGGAAILAGVWLVNRARLP"
     gene            1096..2160
                     /locus_tag="PKK95_00160"
     CDS             1096..2160
                     /locus_tag="PKK95_00160"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOC16644.1"
                     /translation="MNKEIVDLLGEKASDLLEHECKTVSKDMLQLPGPDFVDRVWAPS
                     DRNNRVLGSLQRLFNTGRLAGTGYMSILPVDQGIEHSGGASFAKNPAYFDPENIVKLA
                     VEGGCNAVASTFGVLGAVSRRYAHKIPFIVKINHNELLTYPNKFDQIMFGEVQQAADM
                     GAAAVGATIYFGSEESGRQIVEVAQAFALAHEMGMATVLWCYLRNSAFKKDKDYHVSA
                     DLTGQANHLGVTLQADIIKQKLPENNGGFKAMNTGSSSYGKLDERIYTQLTTDHPIDL
                     TRYQVANCYMGRAGLINSGGASGKSDFPEAAITAVVNKRAGGMGLISGRKAFQRPMAE
                     GVKLLNIIQDVYLDKDITIA"
     gene            complement(2186..4615)
                     /gene="secA2"
                     /locus_tag="PKK95_00165"
     CDS             complement(2186..4615)
                     /gene="secA2"
                     /locus_tag="PKK95_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HOC16645.1"
                     /translation="MHIRRAYLHAAGVPIDHDLKPYAQALAAVEAIETASLSDAALAE
                     RATAAREAAASGRPLDDLLPEVFALVREASARLLGLRPFDVQVEAAVALHRGRLAQLA
                     TGEGKTLVAVMPAVLNALTGRGVHVFTANDYLAERDAGWMGPVYRFFGLTPSFVGQRL
                     SREERRRAYAADVTYVTAKEAGFDFLRDHTAAGPADLVQRPPHFAIVDEADFILIDEA
                     RVPLVIAGAAPDAGVDHRAVAEAAEALVAGRDFDADEYERTVVLTEAGFAHASRLLGA
                     DLTSPDRRLLLSALHVALHARVLLHRDRDYIVRDGRIELVDEFTGRVADNRRWPHGIH
                     SAVEAKEGLAVSAEGRILGSIPIQHFVRRYPRLAGMTATAEPAAQEFDEFYGLRTVVF
                     PPHKSGVRRDEDDEVFTHREAKNAAIVAEIAEVHATGRPVLVGTASVAESEALAEALS
                     AAGVPCQVLNARHDAREAAIVATAGTTGAVTISTNMAGRGTDIVLGAGDPADRARVEA
                     LGGLYVVGTNRHESRRVDDQLRGRSGRQGDPGSSRFFISIEDDLIKRYGVMSLIPPRH
                     RPARQDGPLRDPVVVCEIARAQRIIEGQNFEIRRTLWRYSEMVDDQRRMVYERRQAFL
                     RDEADPDVCRARAPEQYAALVRAAGEAAVRRAEQRLIVRALDQAWTDHLAFVEDVREG
                     IHLQRYGGKDPLTEFSRQLIEAFDAMMHRVEDETIGLFARLRASEEGIDFSGIGPSRS
                     SATWTYLVDDNPFSPLGSSLLASRTLATAAGILGVIYWPVTLVTAITIFWRRWSKKRK
                     GGRQVERGEQG"
     gene            4689..5027
                     /locus_tag="PKK95_00170"
     CDS             4689..5027
                     /locus_tag="PKK95_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC16646.1"
                     /translation="MTPSRDDTSTLLRGTLGLLILRTLELQPLHGLAIADRIAQVTRG
                     TFRVPAGSLFPALHRLEQGGWIRGEWTFTGDGRRVKTYSLTAPGRRKLATEQRNWART
                     VAAMSQVLGS"
     gene            5060..7771
                     /locus_tag="PKK95_00175"
     CDS             5060..7771
                     /locus_tag="PKK95_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC16647.1"
                     /translation="MPLGYRLWSLYRTLFRRDRVERDLDAELTSFVDELAARNAANGM
                     SPGEARRSALLEAGGVEQVKERVRDARTGVWLEQLLQDARIALRMLARSPGFTAVAVA
                     TMALGIGANTAIFSVVDGVLLRPAPFRDIDRLAMVWETDRHSGTTREPASVPDYLDFK
                     ARSSSFDAIAGLSAREVNLSLEGTDPVRLAAMGVTGDLLSMVGVTPVAGRGFDAGEEQ
                     LGGPDVALISESLWQRAFGRDPNVVGRTLRLDEKPHVVVGVLPDAADFGTLQILGAAA
                     YARGFADRDARAHVEVWLPLRPDPVSYPRDTHGVFMLGRLAPGAALSTAAQESTAIAA
                     DLERAYPSNAGRGVFVEPLEEVVFGPVRPPLFVLLGAVGLVLLVASVNVANLLLARGA
                     ARRREAAIRSALGASGSRLSRQFLVEGVVLMLIAGAAGGLLALGGVKALVGIAPGDVP
                     RLSMVTLDLRVLGIALAVSIAVGLVFGMVPTLQARRLDLQSTLKGEGPAQASSGRSRR
                     RLRSLLVAGEVAMAVMLVIAAGLLLRSFWELWRVDPGFRAAGTLKAEYQLPPTRYPVS
                     FAAFPDFKEMHAFTAALLREAAADPAVESAAIAGNHPLDPGFTNSFQVVGRESEARNW
                     PEISVRRVTPGYFRTVGLALQDGRLLEERDETREAPVLLLNEAAAERFFPGTASLGAR
                     IRLWGTERTVVGVVANERIHGLVEAAPPCVYLPLAQAPSFNGAGVLLLRTSGRDPLAL
                     APSVRAMVRKVDPELAVFGIEPLSETLSRSVSTRRFTMLVVGLFAFLALALAAIGIHG
                     VLSYGVALRTREIGLRMALGADAAGVVRLVIGEGLVLALAGLALGLAGALALTRVLAT
                     LLFGVSPTDPATFAAIALFLLLVAAAASGIPAWRAAHIDPVVALRSE"
     gene            complement(7833..8795)
                     /locus_tag="PKK95_00180"
     CDS             complement(7833..8795)
                     /locus_tag="PKK95_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16648.1"
                     /translation="MIKEITLRELDTERFIEEKVAEIRAAVGDGEAISALSGGVDSST
                     VTMLGHRALGPRLKSVFIENGLMREGEAERVAGLFGSLGVPVDIVDARDDFFAALAGL
                     TDPEEKREAITQTFYKKVFGRIVRESGARSLLQGTILTDVDETVAGIKRQHNVFEQLG
                     IDPQEAFGYRILEPLVQLRKDGVRAVGRALGLPAELFNRIPFPGPALAARVIGEVTRE
                     RVDTVRKATTVVERLLAGVGAFQYLAILHEDRVTGMRGGKRQFGGQIEVRCWDSLDAR
                     QATPTNLPYELLRRIADEILEAVPGVVSVTYNIATKPPSTIEAV"
     gene            8883..9650
                     /locus_tag="PKK95_00185"
     CDS             8883..9650
                     /locus_tag="PKK95_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16649.1"
                     /translation="MPLLAVTRDLSPSFEQCQLTHLARQPIDLARARTQHEAYERCLA
                     SLGCRVERLPAGDDLPDSVFVEDCAIVLDEIAVLTRPGAASRRPEVPAVEAALRPYRP
                     SVRIAAPATIDGGDVLRVGRTLYVGLSTRTTELAVEQLEGLVGPFGYQVVAAKVHGCL
                     HLKSAATEVAPNLLLVNPAWVGDWARAFDRIEVDPAEPFAANALRVGDALVYPCAFPR
                     TRARLEARGLDVRPVDVSELAKAEGAVTCCSLVFEAP"
     gene            complement(9691..11649)
                     /locus_tag="PKK95_00190"
     CDS             complement(9691..11649)
                     /locus_tag="PKK95_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOC16650.1"
                     /translation="MPNPVRRFLVAALAALATAMAGSLAAQRQAGLTPDQAAQRWRAE
                     RELQEVAEVERKVMMPMRDGVRLATDVYRPRNGGAKVPTIFVRTPYNFNHWDVQNGVP
                     RDMTQVLAAVKRGYAYVIQNERGHFFSEGHYDILGAPTTDGYDALDWIAKQPWSNGRV
                     GTTGCSSTAEYQMAIAAMGHPAYAAMNVQGFGAGVGRVAPYFEQGNWYRGGAVQMLFI
                     AWLHGEQNQLRPMFPRDMPREDLVAASRYFDLAPRIAQVDWSQALWHLPVQDILKNVD
                     GQRGIFSDPMPVPTGGRMIQRAPNDPAWYKGGLFHDNDTINVPGLWFMSWYDVSVGPN
                     LALYNHVRKTARPEIAEQQWAIIAPVAHCAYTRATEDTVVGERSMGDARFDYQEVIYG
                     FFDRYLKGEKGGRLESLPKVTYFTMGSNRWQESPTWPPEGAAPMTFYLASGGKANSLH
                     GDGVLAARPPEADTPDAFTYDPMNPVTSYGGNVCCMGTAVTAGSFDQRKMEARHDILV
                     YTSEPFEQGIELSGPIEPTLYVSSDAKDTDFTVKVLDVYPDGRAYNLDESIQRMRYRE
                     GYDAPVWMQPGKVYKVTLQPLTTSNYFAPGHRLRIEVSSSNFPRFDRNLNTGGNNYDE
                     SRGVVAHNVVHHSKQYPSQVTVTAVRRK"
     gene            11782..14211
                     /locus_tag="PKK95_00195"
     CDS             11782..14211
                     /locus_tag="PKK95_00195"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOC16651.1"
                     /translation="MNKALLVAVLAGCTAVVSSQPRADYPAAPVPFTDVHLSDSFWAP
                     RLERNRSVTIPHNLAQLTESKRVENFDRAAAAMASGREGQFCSVFPFDDSDVYKAIEA
                     ASYALAIKPDPALDARIDELIAKVAKSQEPDGYLYTARTIGGPPPQPWVGSQRWSYLY
                     LSHELYNVGHLYEAAAAHFQATGKRSLLDIAIKSADLVAKEFGPGKRTNPPGHQEIEI
                     GLVKLYRVTGNAAYLDLAKFFIDVRGRTDARIPFELDGRTQLLYGEYAQDDKPFVEQT
                     EAVGHAVRAGYLYAGAADVAALRQDKAYMAALERIWADVVGKKLYITGGIGAAGAWEG
                     YGPAYRLPNASAYAETCANIATFLWNSRMQRLALDAKYADVMERILYNGVLSGLSLSG
                     DRFFYPNPLASFGQHERTPWFTCACCPPNVARVLTSVPGYFYAASQDSVYVNLFAEGR
                     AAMSPRGTKLELRQKTEYPWQGDILIEVDPERPVAFRLHVRIPGWARNQPVPSDLYRY
                     IEPPAGVPVLRVNGSEVKPPVEKGYAVIDRTWKSGDKVELSLPMPIRRVVAHDAVEAD
                     RGRVAIERGPLVYCAEGPDNDGRAANLVLPDGAALAAEKRPDLLGGVVVIKGVGEAVA
                     EKGGRVGSAPKPVTLIPYYAWANRGKAEMAVWLARDPSRARPVREPGLAAKAKVAASP
                     KAAYPQRVNDQYDPESSDDGTGYMHWYPQKGNLAWVEYAFDAPVTVSESSVYWFDDTG
                     GGSVRVPASWRLLYKDGQEWKPVVLKEQPGVARDRWNTVTFAPLTTSALRLEIQAQKD
                     WSVGVHEWSIR"
     gene            14229..14717
                     /locus_tag="PKK95_00200"
     CDS             14229..14717
                     /locus_tag="PKK95_00200"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOC16652.1"
                     /translation="MHYAWEDSPVGRLLIGGDERGLRFVLFEEGRAPVVPEPGWREDP
                     AAVADAVTQLREYFAGVRRRFDLPLAPAGTPFQLRVWEALRQIPYGETVSYGELARRV
                     GCPNGSRAVGLANGANPLSIVVPCHRVVGAGGRLVGYGGGLGAKRWLLALEQGSRRML
                     NL"
     gene            14798..16123
                     /locus_tag="PKK95_00205"
     CDS             14798..16123
                     /locus_tag="PKK95_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC16653.1"
                     /translation="MNRRDFMKTTVVATAASAIGTRAAGAKTVQANDRIQVGYIGCGA
                     RAHQLMDATVAQPGIEVVGLCDAYAGRIERAQARVGGRAQVYRDYKELLANPAIDAVF
                     IVTPDHWHKTMTLDALAAKKDIYIEKPFTFTVDDGVALVDAVKKSDRILQVGSQSVSS
                     AEYGTVRELIQGGKIGKVVMVRAAYNRNSDSGAWLYPIPPDASPKTVNWEQFLGPAPK
                     RPFDLARFFRWRCYWDYSGGVATDLFVHLVSWMHFATGAKAPKMVVATGETYKYGKTH
                     EVPDTVNALLTYPEGFTANLTCTFNNEQGAESGMEILGTNGSLVVRGGTVTFKPEPRR
                     EDNRWVVASWPEAMEKAYYDDPKVQAIESPFLWDPKMAGGVETWVAEGRDDTYTHIAN
                     FFASVKSRKQPVEDALFGHRAAACAHMVNRSIREKTFVEWDAANERMKG"
     gene            16140..17033
                     /locus_tag="PKK95_00210"
     CDS             16140..17033
                     /locus_tag="PKK95_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC16654.1"
                     /translation="MIRSRREFLSDSAVVLAGAAVLGDPATLLAAPPPAKWPIGCFNR
                     PWAQWPFAETLKGVKAAGYGLTGLLTRTKMDRFIADDATPEYLEGLKAAIAASGVKAV
                     MGALRSRHDVPLEQTTASLHKQIDNAAFLGLQSVMTFGIDNPAQLDHYFKAMADAAAY
                     GQEKGVQVVMKPHGGSSGASDEIIAAMKKVNHRNFSIWYDAGNIIYYTGKDPIEELKP
                     IVQYVTGFCAKDCGQPKGDVMIQFGTGKVDFRGVFKVMKDAGFNGPIMVEGVAPGETP
                     EAVAANARANREFLEKVLASL"
     gene            17073..18005
                     /locus_tag="PKK95_00215"
     CDS             17073..18005
                     /locus_tag="PKK95_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC16655.1"
                     /translation="MPHHTTRRDFLKASVAGTLGAAAGLRAIGLGAQGSVAGRLAVCS
                     WSLQPASADDLFSKLAATGLTRVQIALDPIRTNEGGGWADFGARASKRGVMLVSGMIA
                     TVGEDYSTLESIRRTGGVVPDATWPETWKNIQADLELASKLGLGLVSFHAGFLPHEES
                     DPTFATLQGRLRQIADLAASKKVQVALETGQEEAATLATFLKKLERQNVGVNFDPANM
                     ILYDKGDPVAALKVLSPWLRQVHLKDAVRTKTPGEWGEEVRLGTGQVNWKAFFSTLDS
                     VGFKGNLCIEREAGKERVADIRAAREYVEKLVGK"
     gene            18108..19424
                     /locus_tag="PKK95_00220"
     CDS             18108..19424
                     /locus_tag="PKK95_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HOC16656.1"
                     /translation="MPLVIVALGIAALLALILFLKLNSFLAFVIVSLGVALAEGMGLH
                     EAVASIEKGIGDTLGILVMILGLGAMLGKLVADSGAAERITSSLIGLFGLGRVQWALM
                     LTAFIVGVPLFYDVGFVIMMPIVFAVVASTGVPLLYVGLPTLASLSVTHGYLPPHPAP
                     TAIATMFGADIGKTLVYGVLIAIPAIIVAGPILSRGLRKVNARPMDEFTAAPSAVERS
                     LPGFATSVVSALLPVILIGSATIAAYLLPEGNAVRRVLGWAGNPVIAMLVSLLLAIYT
                     LGLARGREMGDIMASLAHSISSLTMVMLIIGGAGALKQVLVDSGVSAYIGGLLKGSAV
                     SPLVLGWLIATVLRVCVGSATVAGITTAGIILPLVSTSGVNPELMVLAIGSGSLMLAH
                     VNDGGFWLVKEYFNLSIVDTIKTWTVMETAVGVTGLLGVLVLNAFI"
     gene            19448..20524
                     /locus_tag="PKK95_00225"
     CDS             19448..20524
                     /locus_tag="PKK95_00225"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOC16657.1"
                     /translation="MAPLPLIFDAHLDLSMNALEWNRDLRWTVDEIRASERGMADKPD
                     RGRGTVSFEAMRRGNVGICVATLIARFVRPGNQIPGWHSQQQAWAQTQGQLAWYRAME
                     EAGEMAQITTLAELERHLKLWEEGGRSGPIGYVLSLEGADSLVSLEHLARAHARGLRA
                     VGPAHYGPGIYAQGTNACGGLGVKGRELLKEMERLNVILDVSHLCDESFWESLEVFGG
                     PVWASHSNCRSLVPHVRQFSDDQLKALIERGAVVGAALDAWMLAPGWERGRSTPRGMD
                     VTLARVVDHIDHVCQLAGNSRHAGIGSDLDGAFGTEQCPADVDTIADLQKIPSLLAAR
                     GYSAEDVENVLSANYLRFLREAWR"
     gene            complement(20525..22390)
                     /locus_tag="PKK95_00230"
     CDS             complement(20525..22390)
                     /locus_tag="PKK95_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16658.1"
                     /translation="MPTQSLTARERAEQFLEIASDFHLGSLLTESAHPRTADLSAVAR
                     RSTEEGLAALFDVDGDVVHKYREWSASGQAARMASAALETLRRGGRLFFTGCGATGRL
                     SIQLDAAWRAFWQNWAARGLEQPAPGGWEHRTRGAMAGGDYALIKSVEGFEDFAPFGQ
                     RQVQDLGLEKGDTLFAITEGGETSFVIGTAWQALETGARVFFVYNNPDEDLRPVRRSR
                     EVLDEARIEKVNLASGPMAITGSTRMQATSMELLAMLTVLEMVVRELVGERGRHEAGV
                     GRPEEVPAEIRSGLEALHGTLCGDAILGPLAGLVETEERIYRRGGRTSYFAHTLAVDV
                     LTDTTERSPTFCTPPFRKWDDTEAAESWAFLFTGEQRTEGAWRTLLRREPQTIEWTER
                     DLRKLLDAEAASRQAAVVREIGHHDLMRFRIGLDGLQHRLTRAGDAVTVVVTTSDLPL
                     VKEAGSTFSRQIEAATAVEADAALIAVGRHADLHGLRTSAAAQAAGQTVVLPVADTPF
                     LLDPLVRIGVKMLLNALSTCTMVRLGRVLGNRMIWVVPSNLKLIDRSTRYISDLAGVS
                     YEAACHALFEVVEYVEPRRAAGKVYPPPVGVATMRLRHGIGLEEAESRLEELDAR"
     gene            22772..23668
                     /locus_tag="PKK95_00235"
     CDS             22772..23668
                     /locus_tag="PKK95_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC16659.1"
                     /translation="MIGVALALVAPVALAASAPVQGDSPIGIFLRCTGQDDPLRALQA
                     VKSMGVSTIQVSRLPDRFYSPEGAREFAGMLRDTGIRADSVVVVFDGESYRDRDAVVR
                     TVGFRPLHLIPERLAYARRCVDFARAIGARIVTFHMGFLPADPEDEAYRAMLKAVTDI
                     ASYAASQDVTISLETGQETGEQLAAFLNEITVARVGVNFDPANLVIYGVDSPSRALQH
                     FVTRVTSVHVKDALLPEKPGAMGREVRPGEGRAELAECFLMLKASGFRGALVIENYVA
                     RGLKTDPMDELRGAKAFIEKTW"
     gene            23727..24740
                     /locus_tag="PKK95_00240"
     CDS             23727..24740
                     /locus_tag="PKK95_00240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOC16660.1"
                     /translation="MFSEGSLAQRQVEGNAGEAARRGRVGGVLIRVGIAVLMTALALV
                     ASEWGLRYHYRGVGSSGHDGDYFSKRPEVRRNSLGFREREIPPKTPDQYRIVVVGDSF
                     TFGQGLDERDRLSNVLATHLGPRYEVFNFGRRGANLDDHLAILDRALSVSPDFVLLQL
                     FTNDFELSEARRPRHYPLLPAAADRTMGRSSLTYLLVRRGWIRLQEVVGLADSYAGYL
                     ERRLRDPASRDSVKTSALLRRLFRMARAAGAGVGVVMFPATDTFGPGGSGYPYGYLHE
                     RVRLICRDQGVGCVDLFSALSRVPDPRIWWVSAVDAHPSAAANRRAAGEVVAAFASEW
                     GGR"
     gene            24766..26610
                     /locus_tag="PKK95_00245"
     CDS             24766..26610
                     /locus_tag="PKK95_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOC16661.1"
                     /translation="MSNPVFVLGISGFYHDAAAALVRDGEIVAAAQEERFTRVKHDPG
                     FPQLAIDYCLREGGIEPGDLDAVAFYEKPLTKFERLLETYLSFAPSGFASFRQALPIW
                     LRQKLHLPRVMDRGLRGGFKGRYVFPEHHESHAASAFFPSPFEEAAILTIDGVGEWAT
                     AALGFGRGNRVELTHELRFPHSLGLLYSAFTYYTGFKVNSGEYKLMGLAPYGEPRYAE
                     LILEKLLDLKEDGSFRLDLQYFNYCQGLTMTSELFHRLFGGPPRQPESPVTDRDMDLA
                     ASIQVVTEEIVSRMARHLHGLTGMKNLCLAGGVALNCVANGRLRREGPFEGLWIQPAA
                     GDAGGALGAALLVWHQLMGRERRPRSCDSMQGALLGPAFDDAQIKAVLDRAGACYHQY
                     DDETMLEQVVSLLASEKVVGRLQGRMEFGPRALGSRSILGDARSTRMQSVMNLKIKFR
                     ESFRPFAPSVLQEHAHEYFSMDPEVESPYMLLVADVRPDKRVNDDGRRAGLRGMELLK
                     AKRSVVPAITHVDYSARVQTVERDRHASYHRLISRFHEATGCPVVINTSFNVRGEPIV
                     CSPEDALNCFLNTGMDALVMEHFLLLKAEQPASLLGNQEEYLKAFKLD"
     gene            26627..27079
                     /locus_tag="PKK95_00250"
     CDS             26627..27079
                     /locus_tag="PKK95_00250"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOC16662.1"
                     /translation="MNFRDLNLRPNDRELRQFSALWLVGFGLLGAIVAWRAGAFASGI
                     PPGWHAPWRLPIALWGIAAMGAAVGLAVPRAIRPVYVAWMVAAFPIGWAVSQLLLALV
                     YYVLFTAFGLVFRIAGRDALGRSFDRGASSYWVARKPASSLERYFRQF"
     gene            27091..27291
                     /locus_tag="PKK95_00255"
     CDS             27091..27291
                     /locus_tag="PKK95_00255"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOC16663.1"
                     /translation="MVDRDSVDRFEKAAAAPRASLAAELWQFLAHNKKWWLLPIVIVF
                     ALLGALMLLGGTAAAPFIYTLF"
     gene            27556..28080
                     /locus_tag="PKK95_00260"
     CDS             27556..28080
                     /locus_tag="PKK95_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HOC16664.1"
                     /translation="MARFIRETDPYPHPIVIHTYPHLQDKVYVPLLGAQSGLSGISLQ
                     NHWRTAHQRVLKWITESEKAGRPWVVANDEQNPHFTGVPPDPGYEGHDGVARPGKIYV
                     VYLPDGGTTALDLGPAKAEYSVRWYDPRNGGPLLPGSVTSVTGPGVATLGQPPRDPGD
                     DWVILVRRRGEDTE"
     gene            28086..28955
                     /locus_tag="PKK95_00265"
     CDS             28086..28955
                     /locus_tag="PKK95_00265"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC16665.1"
                     /translation="MKHARSMLLVLLVGAVGAGAAAAQGDADRKEWVPIFNGKNLDGW
                     DVKITGHDLNDNAGNTFRVENGVLKTAYDAYGQSFGNRFGHLFYRQKLSHYVVAVEYR
                     FVGEQAPGAPDWAFRNSGVMVHSQSARSMTKGQDFPVSIEVQLLGGPETGERTTANLC
                     TPGTNVEMDGKLVTQHCVNSTSRTYRGDQWVRVEVEVLGGASIRHIVEGQTVLAYQKP
                     QAGGGNVTSADPSVVKDGALLEEGYIALQSESHPIEFRKVELLNLVGCTDPKAANFKS
                     YYVKSDNAACRYR"
     gene            29113..29793
                     /locus_tag="PKK95_00270"
     CDS             29113..29793
                     /locus_tag="PKK95_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOC16666.1"
                     /translation="MWSYFAKGGPVMWPLLVLSILAVVVIVWRWLALREATRNAASLM
                     AALKERLLARDPMGAVAACDAHPGAVASIVRAGLLRLGRPKDEIELGLQDASAHELMA
                     LERGLPVLATVAMVAPLLGFLGTVTGMINSFDALAAVGLNNPAAVASGISEALITTAA
                     GLMIAIPVQMAYNYFVVRVNVTVRDMEAAANLVLEALTDVRLSETGGERAAAPRLAVP
                     NPNPAAAR"
     gene            29806..30231
                     /locus_tag="PKK95_00275"
     CDS             29806..30231
                     /locus_tag="PKK95_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC16667.1"
                     /translation="MRLHRPRTTLRGVIPTATMADIAFLLIIFFMVTTAYSLDRTALE
                     LPQTAEQQQAPKGAALVVIAPDDTFRFSAGEEDAKLVEGLEGLGTAVRQVTAANRLQP
                     FSIKADRRVKYRTIDAVLEQLRQAGAENITLLSRGENER"
     gene            30228..30659
                     /locus_tag="PKK95_00280"
     CDS             30228..30659
                     /locus_tag="PKK95_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC16668.1"
                     /translation="MRVPRREVPSEIPTASMADVAFLLIIFFLVASFYSVTRGVQFSL
                     PRHDEATVNVEPEASVLVEIAPDASLRVDGRSMPLAHLPGYLRPRLVANPAKPVIIRT
                     DLDASYSAMTDVFDELRQAGVRNVVIPTQAELALYGLEGQR"
     gene            30656..>30894
                     /locus_tag="PKK95_00285"
     CDS             30656..>30894
                     /locus_tag="PKK95_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16669.1"
                     /translation="MTDQPQARPPDRREGRAREELALAERWLRRDPRDRKWVARGVAV
                     ALLLHAGVLFARMPGWGAEPKRVEPPQEQAMQVQFL"
BASE COUNT         4595 a        10658 c        10883 g         4758 t
ORIGIN      
        1 gccggcttgc cgtgcgggag atccctcgca gcgggaagcc cgaggagctg ctcgaccggt
       61 tcgggatctc tgcggcgcgg atcgtggcgg cggtcaggga aggcgtgctc gattgaccag
      121 ccagacgccg gcaaggatgg ccgccccccc ggccacgacc gccggcgtca ccggctcgcc
      181 gagcacgcgg ttggcgacgg cgagggttac gatcggctgg atgtactgga acgaggcaag
      241 cgccgaggcg tcggtctcgg cgagcgcgtc gtaccagaag gcgtaggcga cccccgaaca
      301 ccccacgccg aggaacacca ggctccacca tccgcccgcg gtgaggctgg gcaacgcctg
      361 ccagccaagg tccaccgcgg cgagcggcag cgaggcgagc cacccgaagg ccagcacgtg
      421 ggtcatcgtg acggccggcg ggtagcggcc gatcacgcgc ttcgaccagg tggtgaagag
      481 ggccatgttg gcggcgctga gcagcatgag cccgtcgccg ggggctgggt tcgcgacgag
      541 tccccggagg tcgaacccgc gtgcggccac gaccagcgca ccggcggatg ccgccgccag
      601 gcccagcacc ttgtggcgcc cgaacggctc gccgagcatc aggcgggcca ggacggcggt
      661 gaacaccggg atgagggcaa tcatccagcc cgagttggtc gccgtggtcg tcagcaggcc
      721 gaccgactgc agcagctggt gcacggtcac gccgatgacg ccgagcagcg cgagccaggc
      781 gaggtcgccc cggtcggcga ggcgcgtctg gcgccgccac gccagcagcg ccaggaggaa
      841 ggcgagaccg aggccgaagc gggccgcgat gatcgccagc ggcgacagtt cgcgcagcgc
      901 gatcttggtc gccacgaacg aaccgcccca gagggcgatg gcggcgagca ctttcaggat
      961 ggtgaccggc cggcggaggg tctgcggggc cgggacgatc gcgtgcatcg gcgcagtgta
     1021 cagtgggaat cggccggccc gctgccactt gatgttgccg gccggttgtg tgaggcttga
     1081 ggcgaggagg agcgagtgaa caaggaaatc gtggatctgc tcggcgaaaa ggcaagtgac
     1141 ctgctcgagc acgagtgcaa gacggtgtcg aaggacatgc tgcaactgcc cggccccgac
     1201 ttcgttgacc gggtctgggc gccttccgac cgcaacaacc gcgtgctcgg cagcctgcag
     1261 cggctcttca acacgggccg cctcgccggc accggctaca tgtcgatcct gccggtcgac
     1321 cagggcatcg agcattcggg aggagcgagc ttcgccaaga acccggcgta cttcgacccc
     1381 gagaacatcg tcaagctggc ggtcgaggga ggctgcaacg ccgtggcctc gaccttcggc
     1441 gtgctcgggg ccgtgtcgcg ccgctacgcg cacaagatcc ccttcatcgt gaagatcaac
     1501 cacaacgagc tgctgaccta cccgaacaag ttcgaccaga tcatgttcgg cgaggtgcag
     1561 caggcggccg acatgggcgc ggccgccgtg ggcgcgacga tctactttgg atccgaggag
     1621 agcggccgcc agatcgtcga ggtggcgcag gccttcgcgc tggcgcacga gatggggatg
     1681 gcgaccgtgc tgtggtgcta cctgcgcaac tcggcgttca agaaggacaa ggactaccac
     1741 gtcagcgccg acctgacggg gcaggccaac cacctgggcg tcaccctgca ggccgacatc
     1801 atcaagcaga agctgcccga gaacaacggc ggcttcaagg cgatgaacac cggcagctcc
     1861 agctacggca agctcgacga gcgcatctac acgcagctga ccaccgatca cccgatcgac
     1921 ctgacgcgct accaggtggc caactgctac atgggccgcg ccgggctcat caactcgggc
     1981 ggggcgtcgg gcaagagcga cttccccgaa gcggcgatca ccgcggtcgt gaacaagcgg
     2041 gcgggcggca tgggcctcat ctccgggcgg aaggcgttcc agcggccgat ggccgagggc
     2101 gtcaagctgc tcaacatcat ccaggacgtc tacctcgaca aggacatcac gatcgcgtag
     2161 gcagcctgat tcggcgagca gggcttcagc cctgctcgcc gcgttctact tgacgaccgc
     2221 ccttcctctt cttgctccat cgcctccaga aaatcgtgat cgccgtcacc agcgtgaccg
     2281 gccagtagat caccccgagg atcccggcgg cggtcgccag cgtgcggctg gcgagcagcg
     2341 acgagcccag cggcgagaac gggttgtcgt cgaccaggta cgtccaggtc gccgacgacc
     2401 gcgacgggcc gatgccggag aagtcgatcc cctcctcgct cgcgcggagc cgggcgaaga
     2461 gcccgatcgt ttcgtcctcc acgcggtgca tcatcgcgtc gaacgcctcg atcagctggc
     2521 ggctgaactc ggtgaggggg tccttgccgc cgtagcgctg caggtggatt ccctcgcgca
     2581 cgtcctcgac gaacgccagg tggtcggtcc aggcctggtc cagcgcgcgc acgatcaagc
     2641 gctgctcggc gcggcgcaca gctgcttcgc cggcggcccg gacgagggcg gcgtactgct
     2701 ccggcgcccg cgcccggcag acgtcggggt cggcctcgtc gcgcaggaag gcctggcgcc
     2761 gctcgtacac catccgccgc tggtcgtcca ccatctcgga gtaccgccag agcgtccggc
     2821 ggatctcgaa gttctgaccc tcgatgattc gttgcgcccg cgcgatctcg cagacgacca
     2881 ccgggtcgcg cagcggcccg tcctgccgcg cggggcggtg gcgcggcggg atcagcgaca
     2941 tgacgccgta acgcttgatc aggtcatcct cgatcgagat gaagaagcgc gacgaacccg
     3001 ggtcgccctg gcgccccgac cgtccacgga gctggtcgtc gacccgccgg ctctcgtgcc
     3061 ggttggtgcc gacgacgtag aggccgccga gggcttcgac gcgcgcccga tcggccgggt
     3121 cgccggcccc cagcacgatg tcggtgccgc gccccgccat gttcgtggag atcgtcaccg
     3181 cgccggtcgt cccggccgtg gccacgatgg cggcctcgcg cgcgtcgtgc ctcgcgttga
     3241 gcacctggca cggcaccccc gccgccgaga gcgcctccgc gagcgcctcc gactcggcga
     3301 cgctcgccgt gccgaccagc accgggcgtc cggtcgcgtg cacctcggcg atctcggcca
     3361 cgatcgccgc gttcttcgcc tcgcggtggg tgaacacctc gtcgtcttcg tccctccgga
     3421 cgccagactt gtgagggggg aagaccaccg tgcgcagtcc gtagaactcg tcgaactcct
     3481 gggccgccgg ttcagccgtc gccgtcatcc ccgcgaggcg cgggtagcgg cgcacgaagt
     3541 gctggatcgg gatggagccg aggatccttc cctcggcgct gacggccagg ccttccttcg
     3601 cctcgacggc ggagtggatg ccgtggggcc accgccggtt gtcggcgacg cggccggtga
     3661 actcgtcaac cagttcgatc cgcccgtcgc ggacgatgta gtcgcggtca cggtgaagga
     3721 gaacgcgcgc gtgcagtgcg acgtgcagcg cgctgagcag caggcgccgg tccggcgacg
     3781 tgaggtcggc gccgagcagc ctcgaggcgt gggcgaagcc ggcctcggtc agcacgaccg
     3841 tgcgctcgta ctcgtcggcg tcgaaatccc tgccggcgac gagcgcttcg gcagcctccg
     3901 ccaccgcccg atggtcgacc ccggcgtcgg gggcggcgcc cgcgatgacc agcggcacgc
     3961 gcgcctcgtc gatcaggatg aagtcggctt cgtccacgat ggcgaagtga ggcgggcgct
     4021 gcaccaggtc ggcggggccg gccgcggtgt ggtcgcgcag gaaatcgaat cccgcttcct
     4081 tggcggtcac gtaggtcacg tcggccgcat aggcccggcg acgctcctcc cgggacaggc
     4141 gctggccgac gaaggaaggg gtcaggccga agaaccggta aaccggcccc atccacccgg
     4201 cgtcgcgctc ggcgaggtag tcgttggcgg tgaaaacgtg cacgccgcgg ccggtgagcg
     4261 cgttgaggac cgccggcatg acggccacca gcgtcttgcc ttcacccgtc gcgagctgcg
     4321 ctaggcggcc gcggtggagg gcgaccgccg cttcgacctg gacgtcgaag gggcgcaggc
     4381 ccagcagccg cgccgacgcc tcgcgcacga gggcgaaaac ctccggcagc aggtcgtcga
     4441 gcgggcggcc gcttgcggcg gcctccctcg ccgcggtggc ccgctccgcg agcgcggcgt
     4501 cggagaggct ggccgtctcg atggcctcga cggccgcgag cgcttgcgcg tacggcttca
     4561 ggtcgtggtc gatcgggacg ccggcggcgt gaaggtacgc acggcggatg tgcatgttcc
     4621 gggaatgcta ctctcgacaa cttggtatag gatatctata tcggtattct atacatgagg
     4681 cccgcagcat gactccctct cgcgacgaca cctcgaccct gctccgcggc acgctcgggc
     4741 tgctcatcct ccggacgctc gagctgcagc cgctgcacgg tctggcgatc gccgaccgga
     4801 tcgcgcaggt gacgcgaggc acgttccggg tgccggcggg ttcgttgttc ccggcgctgc
     4861 accggctcga gcagggcggg tggatccgcg gggaatggac cttcacgggc gacggccgcc
     4921 gcgtcaagac ctactcgctg acggcaccgg gacgccggaa gctggccacc gagcagcgga
     4981 actgggcgcg cacggttgcc gcgatgtcgc aggttctcgg gtcgtagccc gaattgtccg
     5041 cgaacgcgag gaggacggga tgccgctcgg ttaccggctg tggagcctct accgcacgct
     5101 ctttcggcgc gaccgcgtcg agcgcgatct ggacgccgaa ctcacatcct tcgtggacga
     5161 gcttgccgcc cgcaacgcgg ccaacgggat gagccccggc gaggcaaggc gatcggcgct
     5221 gctcgaggcc ggtggcgtcg agcaggtgaa ggagcgggtg agggacgcgc gcacgggcgt
     5281 ctggctcgag cagctgctcc aggacgcgcg catcgcgctg cggatgctcg cccggtcgcc
     5341 cgggttcacg gccgtcgccg tcgcgacgat ggccctcggg atcggggcga acaccgcgat
     5401 cttcagcgtc gtggacggcg tgctgctgcg tcccgcgcct ttccgcgaca tcgatcggct
     5461 cgccatggtc tgggagaccg accgccacag cgggaccaca cgcgagccgg catcggtgcc
     5521 cgactacctc gatttcaagg ctcgcagttc gagcttcgac gcgattgccg gcctgtcggc
     5581 gcgggaagtg aacctctcgc tcgagggaac cgacccggtg cggcttgctg ccatgggggt
     5641 gacgggagac ctcctttcga tggtgggcgt cacccccgtt gccggtcgtg gcttcgacgc
     5701 gggcgaggaa cagctcggcg gaccggatgt cgcgctgatc agcgagtcgc tctggcagcg
     5761 agccttcggg cgcgatccga acgtggtcgg ccgcacgctc cggctggacg agaagcccca
     5821 cgtggtggtg ggtgttctgc ccgacgccgc cgatttcggc acgctgcaga tcctcggcgc
     5881 cgccgcgtac gcccgcgggt ttgccgacag ggacgcgcgg gcccacgtcg aggtctggct
     5941 gccgctgcgg ccggatcccg tgtcgtaccc gcgcgacacg cacggcgtgt tcatgctcgg
     6001 ccggctggcg cccggcgccg cgctgtccac ggctgcgcag gagtcaaccg ccatcgcggc
     6061 agacctggag cgcgcgtatc cctcgaatgc cggccggggc gtcttcgtcg agccgctcga
     6121 ggaggtggtg ttcggcccgg tccggccccc gctgttcgtg ctgctcggcg ccgtgggcct
     6181 ggtcctgctg gtggcgtcgg tcaacgtcgc caacctcctg ctggcgcgcg gggccgcgcg
     6241 gcggcgcgag gccgccatcc ggagcgccct cggtgcgagc ggcagccggc tgtcgcggca
     6301 gttcctggtc gaaggcgtgg tcctgatgct cattgccggg gccgccggcg ggctgctggc
     6361 cctgggcggc gtgaaggcgc tcgtcgggat cgcgccgggc gacgtcccgc gcctgtcgat
     6421 ggtcacgctc gacctgcgcg tgctcggcat cgccctggca gtgtcgattg ccgtcggcct
     6481 cgtcttcggg atggtgccga cgctccaggc ccgccggctc gacctgcagt ccaccctgaa
     6541 gggcgagggt ccggcacagg cgtcctccgg ccggtctcgc aggcgcctgc ggtcgctgct
     6601 ggttgccggc gaggtggcca tggcggtgat gctggtgatt gccgccgggc tcctgctcag
     6661 gagcttctgg gagctgtggc gcgtggatcc cgggttccgc gccgccggca cgctgaaagc
     6721 cgagtaccag ctgcccccca cccggtaccc cgtctcattc gccgcgttcc ccgacttcaa
     6781 ggagatgcac gccttcacgg cggcgctgct gcgcgaggcc gccgccgacc ccgccgtcga
     6841 gtcggcggcc atcgcgggca accacccgct cgacccgggc ttcacgaact cgttccaggt
     6901 ggtcggccgc gagtcggagg ctcggaactg gcccgagatc tccgtccgcc gcgtgacgcc
     6961 ggggtacttc cgcacggtgg gtctggcgct tcaagacgga cggctcctcg aggagcggga
     7021 tgaaacccgc gaggcgcccg tcctgctcct caacgaggcg gcggccgagc ggttcttccc
     7081 gggcaccgcc tcgctcggcg cacgcatccg gctctggggc accgagcgga cggtcgtcgg
     7141 ggtggtggcc aacgagagga ttcacggcct ggtcgaggcg gcccctccct gcgtgtacct
     7201 gccgctcgcc caggctccgt cgttcaacgg cgccggcgtc ctgctgctgc ggacctccgg
     7261 ccgcgatccg ctggcgctgg ccccgtcggt cagggcgatg gtccggaagg tcgaccctga
     7321 gctggctgtt ttcgggatcg agccgttgtc cgagacgctg tcgcgctcgg tctcgacccg
     7381 ccgcttcacg atgctggtcg tcgggctgtt cgccttcctg gccctggcgt tggccgccat
     7441 cgggatccac ggagtgctga gctacggcgt ggcgctgcgc acccgggaga ttggcctgcg
     7501 catggcgctc ggggccgacg cggccggcgt ggtgcgcctg gtcatcggcg agggcctcgt
     7561 gctggcgctc gccggcctgg cgctgggcct ggccggcgcc ctcgcgttga cgcgggtgct
     7621 cgccacgctg ctgttcggcg tgtcgccgac cgacccggcg accttcgcgg caatcgcgct
     7681 tttcctgctg ctcgtcgccg ccgcggccag cggcattccc gcctggaggg cggcgcacat
     7741 cgacccggtg gtcgccctcc gctcggagta atgccgcgtc gcgccaggcg ctacggcgcg
     7801 gcaagccctg agccctgagc cttgagcctt ccttacaccg cctcgatcgt cgacggcggc
     7861 ttggtggcga tgttgtaggt cacgctcacc acgcccggca ccgcctcgag gatctcgtcc
     7921 gcgattcgcc gcagcagctc gtacggcagg ttggtcggcg tcgcctggcg cgcgtcgagg
     7981 ctgtcccagc accgcacctc gatctggccg ccgaactggc gcttcccgcc gcgcatgccg
     8041 gtgacccggt cctcgtggag gatggccaag tactggaagg cgccgacgcc agcgagcagg
     8101 cgctccacca cggtcgtcgc ctttcgtacc gtgtcgaccc gctcccgcgt cacctcgccg
     8161 atcaccctcg ccgccagcgc cgggccgggg aacgggatcc ggttgaacag ctcggcgggc
     8221 aggccgagcg cgcggcccac cgcccggact ccgtccttgc gcagctggac gagcggctcg
     8281 aggatccggt agccgaaggc ctcctgcggg tcgatgccga gctgctcgaa cacgttgtgc
     8341 tgccgcttga tccccgccac cgtctcgtcc acgtcggtca ggatcgtccc ctgcagcagc
     8401 gaccgcgcgc cgctctcgcg gacgatgcgg ccgaagacct tcttgtagaa ggtctgggta
     8461 atcgcctcgc gcttctcctc ggggtcggtc aggccggcga gggccgcgaa gaagtcgtcg
     8521 cgcgcgtcca cgatgtcgac cggcaccccg agcgaaccga agagcccggc gacgcgctcg
     8581 gcctctccct cacgcatcag gccgttctcg atgaacaccg acttcaaccg cggcccgagc
     8641 gctcgatgcc ccagcatggt gacggtggag gagtccacgc cgcccgagag ggcgctgatg
     8701 gcctcgccgt cgccgaccgc ggccctgatc tcggccacct tctcctcgat gaaccgctcg
     8761 gtgtccagct cgcgcagcgt gatttccttg atcatctgag ccgtcctttc gtgccggggg
     8821 cccaggcggc aagccccgtg aatggtgatg cctcacgact gaaagctgat aagttagccc
     8881 ctatgccgct cctggccgtc acccgcgatc tcagcccgtc gttcgagcag tgccagctca
     8941 cccatctcgc ccgccagccc atcgacctcg cgcgcgcgcg aacgcagcac gaggcctacg
     9001 agcggtgcct ggcctcgctc ggctgccgcg tggagcggct gccggccggc gacgacctcc
     9061 ccgactccgt cttcgtcgag gactgcgcga tcgtgctcga cgagatcgcc gtcctcaccc
     9121 gccccggcgc cgcgtcccgg cggccggagg tgccggcggt cgaggcggca cttcgcccct
     9181 atcgcccctc ggtgcgcatc gccgcccccg ccaccatcga cggcggcgac gtgcttcgcg
     9241 tcggccgcac cctgtacgtc ggcctctcca cccggaccac cgaactggcg gtcgagcagc
     9301 tcgaaggcct cgtcgggccg ttcggatacc aggtggtggc cgccaaggtg cacggctgcc
     9361 tgcacctcaa gagcgcggcc accgaggtgg ctccgaacct cctcctcgtg aaccccgcct
     9421 gggtcggcga ctgggcccgc gccttcgacc gcatcgaggt cgatcccgcc gagccgttcg
     9481 cagccaacgc cctcagggtc ggcgacgcgc tcgtctatcc ctgcgccttc ccccggaccc
     9541 gggcgcgcct cgaggcccgg gggctcgacg tgcgcccggt cgacgtctcc gagctggcca
     9601 aggccgaggg ggccgtcacc tgctgcagcc tcgtgttcga ggccccgtga cgcctggggg
     9661 gccgaccccc cagcccccag tcccgagccc ctatttccgt cgcacggccg tcacggtgac
     9721 ctgcgacggg tactgcttcg agtggtgcac cacgttgtgc gccacgacgc cgcggctctc
     9781 gtcgtagttg ttgccgccgg tgttcagatt gcgatcgaag cgcgggaagt tgctgctcga
     9841 cacctcgatc cgcagccggt gccccggcgc gaagtagttg ctcgtcgtca gcggctgcag
     9901 cgtcaccttg tagacctttc ccggctgcat ccacaccggc gcgtcgtacc cctcccggta
     9961 ccgcatccgt tggatcgact cgtccaggtt gtaggcgcgt ccgtccgggt acacgtcgag
    10021 caccttcacc gtgaagtcgg tgtccttcgc gtcggacgag acgtacagcg tcggctcgat
    10081 cgggccgctc agctcgatcc cctgctcgaa gggttccgag gtgtacacga ggatgtcgtg
    10141 gcgcgcctcc atcttccgct ggtcgaacga ccccgccgtg accgccgtcc ccatgcagca
    10201 cacgttgccg ccgtacgagg tcaccgggtt catcgggtcg tacgtgaagg cgtcgggcgt
    10261 gtcggcctcc ggcgggcggg ccgccagcac gccgtcgccg tgcagcgagt tcgccttgcc
    10321 gccgcttgcc aggtagaacg tcatcggggc ggcgccttcc ggcggccacg tgggcgactc
    10381 ctgccaccgg ttcgacccca tggtgaagta cgtcaccttc ggcaggctct cgagccggcc
    10441 gcccttctcc cccttgaggt accggtcgaa gaagccgtag atcacctcct ggtagtcgaa
    10501 gcgggcgtcg cccatgctgc gctcgccgac gaccgtgtcc tcggtggcgc gagtgtaggc
    10561 gcagtgggcc accggcgcga tgatcgccca ctgctgctcc gcgatctcgg gccgcgccgt
    10621 cttgcgaacg tggttgtaga gggccaggtt cgggccgacc gacacgtcgt accacgacat
    10681 gaaccacaga cccggcacgt tgatggtgtc gttgtcgtgg aagagcccac ccttgtacca
    10741 ggccgggtcg ttcggcgccc gctggatcat gcggcccccc gtcggcaccg gcatcggatc
    10801 ggagaagatt ccccgctggc cgtccacgtt cttcaggatg tcctgcaccg ggaggtgcca
    10861 cagcgcctgc gaccagtcca cctgtgcgat tcgcggcgcg aggtcgaagt accgcgaggc
    10921 ggccaccagg tcctcgcgag gcatgtcgcg cgggaacatc ggccgcagct gattctgctc
    10981 gccgtggagc cacgcgatga agagcatctg gacggcgccg ccgcggtacc agttgccctg
    11041 ctcgaagtac ggcgcgacgc ggcccacccc ggcgccgaac ccctggacgt tcatcgcggc
    11101 gtaggcgggg tgacccatcg cggcgatcgc catctggtac tccgccgtcg acgagcaccc
    11161 ggtcgtgccg accctgccgt tcgaccacgg ctgcttcgcg atccagtcga gggcgtcgta
    11221 gccgtcggtg gtcggcgccc cgaggatgtc gtagtgcccc tcggagaaga agtggccgcg
    11281 ctcgttctgg atgacgtagg cgtagccgcg cttcacggcg gcgagcacct gcgtcatgtc
    11341 cctcgggacg ccgttctgca cgtcccagtg gttgaagttg tagggcgtgc ggacgaagat
    11401 cgttggaacc ttcgccccgc cgttcctggg gcgatagacg tcggtggcca ggcggacgcc
    11461 gtcgcgcatc ggcatcatca ccttgcgctc cacctcggcc acctcctgca gctcccgctc
    11521 ggcacgccag cgttgggcgg cctggtcggg ggtgagcccg gcctggcgtt gggcggcgag
    11581 cgagccggcc atcgcggtgg cgagggcggc cagggcggcg acgaggaacc ggcgaacggg
    11641 attgggcatc gggcgctcct cgaatgtgcg ccatacatta caacgaacag gcacggcccc
    11701 gcgtcacggg gcggcccggc tcggggtatt ctacgggggc gtacggcccg tccgagccat
    11761 gagacctgat ccagaagacc catgaacaaa gcacttctcg ttgccgtcct ggccggttgt
    11821 accgcggtcg tgtcgtccca gccccgggcc gactacccgg cggcgccggt tcccttcacc
    11881 gacgtccacc tctcggattc attctgggcg ccgcgcctcg agcggaaccg ctcggtgacc
    11941 atcccccaca acctggcgca gctcaccgag agcaagcgcg tcgagaactt cgaccgggcg
    12001 gccgcggcca tggcgagcgg ccgggagggc cagttctgct ccgtcttccc gttcgacgac
    12061 tccgacgtct acaaggcgat cgaagccgcg tcgtacgccc tcgccatcaa gccggatccg
    12121 gcgctcgacg cgcgcatcga cgagctgatc gccaaggtcg cgaagtcgca ggagccggat
    12181 gggtacctct acaccgcccg gacgatcggc ggcccgccgc cgcagccctg ggtgggcagc
    12241 cagcggtggt cgtatctcta cctgagccac gagctgtaca acgtggggca cctttacgag
    12301 gcggccgccg cccatttcca ggccaccggc aagcggagcc tgctcgacat cgcgatcaag
    12361 agcgccgacc tggtcgccaa ggaattcggc cccggcaagc ggaccaatcc cccgggccac
    12421 caggaaatcg agatcggcct ggtgaagctg taccgcgtca cggggaacgc ggcatacctc
    12481 gacctcgcga agttcttcat cgacgtgcgc ggccgcacag acgcgcggat ccccttcgag
    12541 ctggacggca ggacgcagct gctctacggc gagtacgcgc aggacgacaa gccgtttgtg
    12601 gagcagaccg aggcggtggg ccatgcggtg cgtgccggct acctctacgc gggagcggcc
    12661 gacgtggccg cccttcgcca ggacaaggcc tacatggcgg ccctcgagcg catctgggcc
    12721 gacgtggtgg ggaagaagct gtacatcact ggcggcatcg gcgcggccgg cgcgtgggag
    12781 ggctacggcc cggcgtaccg cctgcccaac gccagcgcct acgccgagac ttgcgccaac
    12841 atcgccacct tcctctggaa ctcgcggatg cagcgcctcg ccctcgacgc gaagtacgcc
    12901 gacgtgatgg aacggatcct ctacaacggc gtgctgtcgg ggctgtcgct ctcgggcgac
    12961 cgtttcttct acccgaaccc gctggcctcg ttcggccagc acgagcgcac cccctggttc
    13021 acgtgcgcct gctgcccgcc gaacgtcgcc cgcgtcctca cctcggtgcc gggctacttc
    13081 tacgcggcgt cccaggacag cgtctacgtg aacctgttcg ccgaggggcg ggcggcgatg
    13141 tcgcctcgcg ggacgaaact ggagctgcgc cagaagaccg agtacccgtg gcagggtgac
    13201 atcctgatcg aggttgaccc cgagcgcccc gtcgcgttcc gcctgcacgt ccgcatcccc
    13261 ggctgggccc ggaaccagcc cgtgccctcc gacctctacc ggtacatcga gccgcccgcg
    13321 ggagtccccg tgcttcgcgt gaacggctcg gaggtgaagc cccctgtcga gaagggctac
    13381 gcggtgatcg accgcacgtg gaagtcaggc gacaaggtcg agctgagcct gccgatgccg
    13441 atccggcgcg tggtcgcaca cgacgcggtc gaggccgaca gggggcgcgt cgcgatcgag
    13501 cgcgggccgc tggtgtactg cgccgagggg cccgacaacg acggccgggc cgcgaacctc
    13561 gtcctgcccg acggggccgc cctggccgcc gagaagcggc ccgacctgct ggggggcgtc
    13621 gtcgtgatca agggcgtggg cgaggcagtt gcggagaagg gggggcgggt cggctcggcg
    13681 ccgaagccgg tgaccttgat cccgtactac gcctgggcca accgcggaaa ggccgagatg
    13741 gccgtctggc tcgcgcgcga tccgtcacgg gcccgcccgg tccgcgagcc cggcctggcg
    13801 gcgaaggcga aggtggccgc ctcaccgaag gccgcctacc cgcagcgcgt caacgaccag
    13861 tacgatcccg agtcgtcgga cgatgggacc ggctacatgc actggtaccc gcagaagggg
    13921 aacctggcgt gggtcgagta cgcgttcgac gccccggtga ccgtgtcgga gtcgtcggtg
    13981 tactggttcg acgataccgg cggcggcagc gtccgcgtcc ccgcgtcgtg gcggttgctc
    14041 tacaaggacg ggcaggagtg gaagccggtc gtcctcaaag agcagcccgg cgtggcccgg
    14101 gaccgctgga acaccgtgac gttcgcgccg ctgaccacca gtgccctccg cctcgagatc
    14161 caggcgcaga aggactggtc ggtcggcgtg cacgagtgga gcatccggta gggcagccgg
    14221 gggaggacat gcactacgcc tgggaggaca gcccggtcgg ccggttgttg attggcggcg
    14281 acgagcgcgg gcttcgcttc gtcctcttcg aggaaggccg cgcgcccgtc gtgcccgagc
    14341 ccgggtggcg ggaagacccg gcggccgtgg ccgacgcggt cacgcagctg cgggagtact
    14401 tcgccggagt gcggcgccgc ttcgacctcc ccctggcccc ggccgggacg cccttccagc
    14461 tgcgggtctg ggaggcgctg cggcagattc cttacggcga gacggtgtcg tacggcgagc
    14521 tggccaggcg cgtggggtgc ccgaacgggt cgcgggccgt ggggctggcc aacggcgcga
    14581 acccgttgtc gatcgtggtg ccgtgccacc gggtcgtcgg cgccggcggc agactggttg
    14641 gctacggagg ggggcttgga gcgaagcggt ggttgctggc gctcgagcag ggaagcaggc
    14701 ggatgttgaa cctgtaaccg ggaacgcgag agcggaggac ggaaacgggg gacattcttt
    14761 ccgccgcccg ttcccgcaat cattgagggg cattccgatg aatcgacgcg acttcatgaa
    14821 gacgacggtg gtggcgacgg cggcgagcgc gatcgggacc agggctgcgg gggcgaagac
    14881 cgtgcaggcc aacgatcgca tccaggtggg ctacatcggc tgcggcgccc gggcacacca
    14941 gctcatggat gcgaccgtgg cccagcccgg catcgaggtc gtggggctgt gcgacgccta
    15001 cgcgggccgg atcgagcggg cgcaggcgcg ggttggcggg cgggcgcagg tctaccgcga
    15061 ctacaaggag ctgctcgcga accccgcgat cgacgcggtc ttcatcgtga cccccgacca
    15121 ctggcacaag acgatgaccc tcgacgcgct ggccgcgaag aaggacatct acatcgagaa
    15181 gccgttcacc ttcacggtgg acgacggcgt cgcgctggtc gatgccgtga agaagtcgga
    15241 ccgcatcctc caggtgggca gccagagcgt cagctcggcg gagtacggca cggtgcgcga
    15301 gctgatccag ggcggcaaga tcggcaaggt cgtcatggtg cgggcggcct acaaccgcaa
    15361 cagcgacagc ggggcctggc tctacccgat cccgcccgac gccagcccga agaccgtcaa
    15421 ctgggagcag tttctcgggc ctgcgccgaa gcggcccttc gacctggcgc gcttcttccg
    15481 gtggcgctgc tactgggact actcgggcgg cgtcgcgacc gacctgttcg tgcacctggt
    15541 gtcgtggatg cacttcgcca ccggcgccaa ggcgccgaag atggtggtgg cgaccggcga
    15601 gacctacaag tacggcaaga cccacgaggt gcccgacacg gtcaacgcgc tgctgaccta
    15661 cccggagggg ttcaccgcca acctgacctg caccttcaac aacgagcagg gcgccgagtc
    15721 gggcatggag attctcggga ccaacggcag cctggtcgtc cgcggcggca cggtgacctt
    15781 caagcccgag ccgcggcgcg aggacaaccg gtgggtcgtg gcctcgtggc ccgaggcgat
    15841 ggagaaggcc tactacgacg accccaaggt gcaggcgatc gagtcgccgt tcctgtggga
    15901 tccaaagatg gcgggcggcg tcgagacctg ggtcgccgag gggcgcgacg acacctacac
    15961 ccacattgcg aacttcttcg cgtcggtcaa gtcgcgcaag cagccggtcg aggatgcctt
    16021 gttcggccac cgcgcggcgg cctgcgcgca catggtgaac cggtcgatcc gcgagaagac
    16081 gttcgtcgag tgggatgccg ccaacgagcg gatgaaggga taagaggagg gcaggtccga
    16141 tgattcgcag ccgccgggaa ttcttgtccg acagtgccgt cgtcctggca ggggccgccg
    16201 tgctcggcga ccccgccacc ttgttggccg cccccccgcc cgcgaagtgg cccatcgggt
    16261 gcttcaaccg cccgtgggcc cagtggccgt tcgccgagac gctgaagggt gtcaaggccg
    16321 ccgggtacgg cctgaccggc ctgctcaccc gcacgaagat ggaccggttc atcgccgacg
    16381 atgcgacgcc cgagtacctc gaggggctga aggccgcgat tgccgccagc ggggtgaagg
    16441 cggtcatggg ggcgctcagg agccgccacg acgtcccgct ggaacagacc accgccagcc
    16501 ttcacaagca gatcgacaac gcggccttcc tcggcctgca gtcggtcatg accttcggca
    16561 tcgacaaccc ggcgcagctc gaccactact tcaaggcgat ggccgatgcg gccgcgtacg
    16621 gccaggagaa gggcgtccag gtcgtgatga agccgcacgg gggcagcagc ggcgcctccg
    16681 acgagatcat cgccgcgatg aagaaggtca accaccgcaa cttctccatc tggtacgacg
    16741 ccgggaacat catctactac accggcaagg acccgatcga ggagctgaag ccgatcgtcc
    16801 agtacgtcac cgggttctgc gcgaaggact gcggccagcc gaaaggcgac gtgatgatcc
    16861 agttcggcac cggcaaggtg gacttcaggg gcgtcttcaa ggtgatgaag gacgcggggt
    16921 tcaacggccc gatcatggtc gaaggcgtgg ccccgggcga gacgcccgag gcggtggccg
    16981 ccaacgcccg cgccaaccgc gagttcctcg agaaggttct ggcgagcctg tagcacgcgg
    17041 cagccgttcg ggctgttcgc gaggagatat cgatgccaca ccacacaacc cgtcgcgact
    17101 tcctcaaggc ctcggtggcc gggacgctcg gcgccgcggc cggcctgcgg gcgatcggcc
    17161 tgggcgcgca gggaagcgtc gcgggccgcc tggccgtctg cagctggtcg ctgcagccgg
    17221 cctcagcaga cgacctgttc tcgaagctgg ccgccaccgg cctgacccgg gtgcagatcg
    17281 ccctcgaccc gatccggacg aacgagggcg ggggatgggc cgacttcggc gccagggcct
    17341 cgaagcgcgg cgtgatgctc gtctccggga tgatcgccac ggttggcgag gactactcca
    17401 cgctcgagag catccgccgc accggcggcg tggtgcccga cgccacctgg cccgagacgt
    17461 ggaagaacat ccaggccgac ctcgagctgg cctccaagct cggcctcggc ctggtgagct
    17521 tccacgccgg cttcctgccc cacgaggaga gcgaccccac cttcgcgacg ctccagggcc
    17581 ggcttcgcca gattgccgac ctggcggcct ccaagaaggt gcaggtcgcg ctcgagaccg
    17641 gccaggagga ggcggcgacg ctcgccacct tcctgaagaa gctcgagcgg cagaacgtcg
    17701 gggtcaactt cgacccggcg aacatgatcc tgtacgacaa gggcgaccca gtggccgcgc
    17761 tgaaggtgct gtcgccctgg ctgcgccagg tccacctgaa ggacgcggtg cgaacgaaga
    17821 cgccggggga gtggggcgag gaagtgcggc tcggcaccgg ccaggtgaac tggaaggcgt
    17881 tcttcagcac gctggactcg gtcggcttca agggcaacct gtgcatcgag cgcgaggccg
    17941 gcaaggagcg ggtggccgac atccgcgccg cgcgcgagta cgtggagaag ctggtgggga
    18001 agtaacggcg aacgcaatct gaagacccat cccacgcgta ccagcggttg agaggcgcgg
    18061 gcgcgcctcg cgcgtctcaa ccgcacccgg ggcctggagt tcaacgcatg ccgctcgtca
    18121 tcgtggccct ggggatcgcg gcgctgctcg cgctgatcct cttcctcaag ctcaactcgt
    18181 tcctggcgtt cgtcatcgtg tcgctcgggg tggccctggc cgaggggatg ggcctccacg
    18241 aggcggtggc gtcgatcgag aaggggatcg gcgacaccct cggcatcctc gtcatgatcc
    18301 tcggcctcgg ggcgatgctg ggcaagctgg tcgccgacag cggggccgcc gagcgcatca
    18361 cctcgagcct gatcggcctc ttcggcctcg gccgcgtgca gtgggccctg atgctcaccg
    18421 cgttcatcgt cggggtcccg ctgttctacg acgtcggctt cgtgatcatg atgccgatcg
    18481 tcttcgccgt cgtcgcctcc accggcgtgc cgctgctgta cgtcgggctc ccgacgctcg
    18541 cttcgctgtc ggtcacccac gggtacctcc cgccgcaccc ggccccgacc gccatcgcga
    18601 cgatgttcgg cgccgacatc gggaagacgc tggtgtacgg cgtcctgatc gcgatcccgg
    18661 caatcatcgt cgcaggcccg atcctgtcgc gcggcctgcg gaaggtgaac gcgcggccga
    18721 tggacgagtt caccgcggcg ccctcggccg tcgagcgaag cctcccgggt ttcgccacca
    18781 gcgtcgtctc ggcgctgctg ccggtgatcc tgatcggctc ggccaccatc gccgcctacc
    18841 ttctacccga gggcaacgcg gtgcgccggg tgctcggatg ggcaggcaac ccggtgatcg
    18901 ccatgctcgt gtcgctgctg ctggcgatct acacgctcgg gctggccagg ggccgcgaga
    18961 tgggcgacat catggcgtcg ctcgcccact cgatctcgag cctgaccatg gtgatgctga
    19021 tcatcggggg ggcgggcgcg ctgaagcagg tgctggtgga cagcggcgtc agcgcctaca
    19081 tcggcggcct gctgaagggg tcggcggtgt cgccgctggt gctggggtgg ctgattgcga
    19141 cggtcctgcg ggtgtgcgtc ggctcggcga ccgtggccgg catcaccacc gcgggcatca
    19201 tcctgccgct ggtcagcacg tccggcgtca accccgagct gatggtgctg gcgatcggct
    19261 cgggcagcct gatgctggcg cacgtgaacg acggcggctt ctggctggtg aaggagtact
    19321 tcaacctgag catcgtggac acgatcaaga cctggacggt gatggagacg gcggtcgggg
    19381 tcaccggcct gctgggcgtc ctggtgctga acgcgttcat ctgaccaggg cgagatagca
    19441 taagggcatg gcacctcttc cgctgatctt cgacgcacac ctcgatctct cgatgaacgc
    19501 gctcgagtgg aaccgcgacc tgcgctggac ggtcgacgag atccgcgcgt ccgagcgggg
    19561 gatggccgac aagcccgatc gggggcgcgg gaccgtgtcg ttcgaggcga tgcgccgagg
    19621 caacgtcggc atctgcgtgg cgacgttgat cgcccgattc gtgcgcccgg ggaaccagat
    19681 ccccgggtgg cactcgcagc agcaggcctg ggcgcagacg caggggcagc tggcctggta
    19741 ccgcgcgatg gaagaggccg gcgagatggc gcagatcacg acgctcgccg agctcgagcg
    19801 gcacctgaag ctctgggaag agggcgggcg ctccggcccc atcggctacg tcctcagtct
    19861 tgaaggggcc gattcactgg tctcgctcga gcacctcgcc cgcgcgcacg cgcggggcct
    19921 gcgggccgtc ggcccagccc attacgggcc cggcatctac gcgcagggaa ccaacgcctg
    19981 cggcgggctc ggcgtgaagg ggcgcgagct gctgaaggag atggagcggc tgaacgtcat
    20041 cctcgacgtc agtcacctgt gcgacgagag cttctgggaa agtctcgagg tgttcggcgg
    20101 gccggtgtgg gcgagccaca gcaactgccg ctcgctcgtc ccgcacgtcc ggcagttctc
    20161 ggacgaccag ttgaaggcgc tcatcgagcg tggcgccgtc gtcggcgccg ccctcgacgc
    20221 gtggatgctg gctccggggt gggagcgtgg gcgctcgacg ccgcggggga tggatgtcac
    20281 cctcgcgcgg gtcgtggacc acatcgacca cgtctgccag ctcgcgggca actcgcggca
    20341 cgccggcatc gggtccgacc tcgacggcgc gttcgggacc gagcagtgcc cggccgacgt
    20401 ggacaccatc gccgacctgc agaagatccc gtcgctcctc gccgcgcgcg gctactcggc
    20461 cgaggacgtg gagaacgttc tgagcgccaa ctacctcaga ttcctgagag aggcttggcg
    20521 ctagtcaccg cgcgtcaagc tcttcgagcc gcgactcggc ctcctcgaga ccgatgccgt
    20581 ggcgaaggcg catcgtggcg acgccaaccg ggggcgggta gaccttgccc gcggcgcgcc
    20641 gcggctcgac gtactcgacc acctcgaaca gcgcgtggca ggccgcctcg tagctgacgc
    20701 ccgccaggtc gctgatgtag cgcgtggagc gatcgatcag cttcaggttg ctcggcacca
    20761 cccagatcat ccggttcccg agcacccggc ccagccgtac catcgtgcag gtggacagcg
    20821 cgttcagcag catcttcacg ccgatccgga ccagcgggtc gagcaggaac ggcgtgtcgg
    20881 caaccgggag gaccaccgtc tgccccgccg cctgcgccgc cgccgacgtc cgcagcccgt
    20941 ggaggtcggc gtgccggcca acggcgatga gggcggcgtc ggcctcgacg gcggtggccg
    21001 cctcgatctg gcgtgagaag gtcgagccgg cctccttcac cagggggagg tcggaggtcg
    21061 tcacgaccac cgtcaccgcg tcgccggcgc gcgtgagccg atgctgcagg ccgtcgaggc
    21121 cgatgcggaa gcgcatcagg tcgtggtggc cgatctcgcg cacgaccgcc gcctgccggg
    21181 aggccgcctc ggcgtccagc agcttgcgca ggtcgcgctc ggtccattcg atggtctggg
    21241 gttcgcgccg cagcagcgtc cgccacgccc cctcggtcct ctgctcaccg gtgaacagga
    21301 aggcccacga ctcggccgcc tcggtgtcgt cccacttgcg gaacggcggc gtgcagaagg
    21361 tggggctccg ctcggtggtg tcggtgagga cgtccaccgc cagggtgtgc gcaaagtacg
    21421 aagtccggcc gccgcggcgg tagatgcgct cctccgtctc gacgagaccg gccagcggtc
    21481 ccaggatcgc gtcaccgcaa agcgttccgt gcagcgcctc gagcccggac ctgatctcgg
    21541 ccggaacctc ctcgggccgc ccgaccccgg cctcgtgcct cccgcgctcg ccgaccagct
    21601 cgcgcacgac catctcgagg acggtcagca tggccagcag ttccatgctg gtggcctgca
    21661 tgcgggtcga gcccgtgatg gccatcgggc cagaggcgag gttgaccttt tcgatgcgcg
    21721 cctcgtcgag cacctcgcgg ctgcggcgga cggggcgaag gtcctcgtcg gggttgttgt
    21781 agacgaagaa gacccgcgcc ccggtctcca gggcttgcca ggccgtgccg atgacgaacg
    21841 aggtctcgcc cccctcggtg atcgcgaaga gcgtgtcgcc cttctccagg ccgaggtcct
    21901 ggacctgccg ctgcccgaac ggagcaaagt cttcgaaccc ctccaccgac ttgatcagcg
    21961 cgtagtcgcc gccggccatg gcgccgcggg tgcggtgctc ccacccgccc ggggcgggct
    22021 gttcgaggcc gcgcgccgcc cagttctgcc agaaggcacg ccaggcggcg tcgagctgga
    22081 tgctgaggcg gccggtggcg ccgcagcccg tgaagaacag cctgccgccc cttcgcagcg
    22141 tctcgagggc agccgaggcc atgcgcgccg cctggccgct ggccgaccat tcgcggtact
    22201 tgtggaccac gtcgccgtcc acgtcgaaga gcgcggccag gccctcctcg gtgctacggc
    22261 gggcgaccgc gctgagatcg gccgttcgcg ggtgggccga ctcggtgaga agcgagccga
    22321 ggtggaagtc ggatgcgatc tcgaggaact gctcggcccg ttcgcgtgcg gtgagggatt
    22381 gtgtcggcat aaggacgagg gacccattgg ggggcgtgcc cctcgattat aggccacggt
    22441 ccggccgggt gcccgatgct ggcgcccgcc gggccgtccg tggtggaata ccgtcgccgg
    22501 cgagcgacgt catgccagcg aacctcaccc cgagcgtgtg gggctcgggc gccttcgacg
    22561 gccagaccgc ccaccgcgat cacgtgctgg cggacggcga catcgtcgag attcacgagt
    22621 aggatcgcga ggtacaccac ggccgccgaa gacatgcgca agcgtcactc ttcgtgttct
    22681 ttgtgatctt tgtggttcaa ttcgtccgtg agggcccgtg gcggccggtc acatagagga
    22741 gagcatggca atgtttcgtc gagcgacagc gatgatcggg gttgccttgg cgctggtggc
    22801 gcccgtagcc ctcgcagcca gtgcgccggt gcagggggat agcccgattg gcatcttcct
    22861 gaggtgcacc gggcaggacg atcccctgcg cgcgctgcag gcggtcaagt cgatgggcgt
    22921 gagcacgatc caggtcagcc gtcttcccga ccgcttctac agccccgagg gggctcgcga
    22981 gttcgcgggc atgctgcgcg acaccggcat ccgcgccgac tcggtggtgg tcgtgttcga
    23041 cggcgagagc taccgcgacc gggacgctgt ggtccggacg gtcgggtttc gcccgctgca
    23101 cctgattccc gagcgcctgg cgtacgcacg ccggtgcgtc gacttcgcgc gggcgatcgg
    23161 cgcgcggatc gtgacgttcc acatgggctt cctgccggcc gatcccgaag acgaggccta
    23221 ccgcgccatg ctgaaggcgg tcaccgacat cgcgtcgtac gccgcgagcc aggacgtgac
    23281 catctcgctc gagactggcc aggagaccgg cgagcagctg gccgcgttcc tgaacgaaat
    23341 caccgtcgcg cgcgtggggg tcaacttcga ccccgccaac ctcgtgatct acggtgtgga
    23401 ctcgccgtcg cgcgcgctgc agcacttcgt cacccgggtc acctcggtgc acgtaaagga
    23461 cgcgctgctg ccggagaagc cgggtgcgat gggccgtgag gtcaggcccg gcgaggggcg
    23521 cgccgagctg gccgagtgct tcctgatgct gaaggcatcg ggcttccgcg gtgcactggt
    23581 aatcgagaac tacgtcgccc gtggtctgaa gaccgacccg atggacgagc ttcgcggcgc
    23641 gaaggccttc atcgagaaga cctggtagac ccgctcatcg ccaaacccgg cccgcctctg
    23701 ctatgctctg aaactcttga gcgggcatgt tcagcgaggg ttcactggct cagcggcagg
    23761 tcgaagggaa cgcgggcgag gcggcgaggc gcgggcgcgt tggcggtgtc ctgatccgcg
    23821 tcggcatcgc cgttctcatg accgccctcg cgctcgtggc cagcgagtgg ggcctgcggt
    23881 atcactaccg cggggtgggc tcgagcggcc acgatgggga ctacttctcg aagcggcccg
    23941 aggtccggcg caacagcctc gggttccggg agcgggagat cccgcccaag acacctgatc
    24001 agtaccggat cgtcgtcgtc ggcgactcct tcacgttcgg gcagggcctg gatgagcgcg
    24061 accgcctttc gaacgtgctc gcgacgcacc tcgggccccg gtacgaggtc ttcaacttcg
    24121 ggaggcgcgg cgccaacctg gacgaccacc tcgcaatcct cgaccgggcg ctgagcgtgt
    24181 cgcccgactt cgtgctgctg cagttgttca cgaacgactt cgagctgtcg gaggcgaggc
    24241 ggccgcgaca ctaccccctc ctgccggccg cggccgaccg gacgatgggg cgttcgtcgc
    24301 tcacctacct tctcgtgcgg cgcggctgga tccgtctgca ggaggtcgtg ggccttgccg
    24361 acagctacgc gggctatctc gagcggcggc tgcgcgaccc ggcgtcgcgt gactcggtga
    24421 agacgtcagc gcttttgcgc cggttgttca ggatggcgcg ggcggccggc gccggtgtgg
    24481 gcgtggtgat gttcccggca accgacacct tcggccccgg cggcagcggc tacccttacg
    24541 gctacctgca cgagcgagtg cgcctgatct gccgcgacca gggggtcggg tgcgtcgatc
    24601 tcttttcggc gctgtcgcgg gtgcccgacc cgcgcatctg gtgggtgagc gccgtcgatg
    24661 cccatcccag cgcggccgcc aaccggcggg ccgcggggga ggtggtggcc gccttcgcgt
    24721 cggagtgggg cgggcggtga tcgaaggaag cgagccggcg gtcccatgag caaccctgtc
    24781 ttcgtcctcg gcatctccgg cttttaccac gacgccgctg ccgcgctcgt ccgcgacggc
    24841 gagatcgtgg cagccgcgca ggaagagcgc tttacccgcg tcaagcacga ccccgggttt
    24901 ccgcagctcg cgatcgacta ttgcctccgc gaggggggca tcgagccggg agacctcgac
    24961 gcggttgcgt tctacgagaa gccgctcacc aagttcgaac ggctgctcga aacctacctg
    25021 tccttcgccc cttccggctt cgccagcttc cgccaggccc tgcccatctg gctccgccag
    25081 aagctgcacc tgccgcgggt gatggaccgg gggctgcgcg gcgggttcaa gggccgctac
    25141 gtcttccctg agcaccacga gtcgcacgcg gccagcgcgt tcttcccctc ccccttcgag
    25201 gaggccgcga tcctcacgat cgacggggtg ggcgagtggg cgacggcggc cctcggcttc
    25261 ggccggggga accgggtcga gctgacccac gagttgcgct tcccccactc gctcggcctg
    25321 ctgtattccg cgttcaccta ctacacgggc ttcaaggtga actcgggcga gtacaagctc
    25381 atgggcctcg ccccgtacgg cgagccgcgc tacgcggagc tgatcctcga gaagctgctc
    25441 gacctgaagg aggacggatc gtttcgcctc gacctgcagt acttcaacta ctgtcagggc
    25501 ctgacgatga cgagcgagct gttccatcgc ctgttcggag ggccgccccg ccagcccgag
    25561 tcacccgtga ccgatcgcga catggacctc gccgccagca tccaggtcgt gaccgaggag
    25621 atcgtctcgc gcatggcgcg gcacctccac ggcctgaccg ggatgaagaa cctgtgcctg
    25681 gcgggaggtg tcgccctcaa ctgcgtcgcc aatggccgcc tgcggcgcga ggggccgttc
    25741 gagggcctgt ggatccagcc tgccgccggc gacgccggcg gcgcgctcgg cgccgcgctt
    25801 ctcgtgtggc atcagctcat ggggcgcgag cgccggccgc gctcctgcga cagcatgcag
    25861 ggcgccctgc tcggtccggc gttcgacgac gcgcaaatca aggcggtcct tgatcgggcc
    25921 ggcgcctgct accaccagta cgacgacgag acgatgctcg agcaggtggt gtcgctgctc
    25981 gccagcgaga aggtggtcgg ccgcctgcag gggcgcatgg agttcggccc gcgggccttg
    26041 ggcagccgca gcatcctggg cgacgcccgc agcacccgga tgcagtcggt gatgaacctg
    26101 aagatcaagt tcagggagtc gttccgcccc ttcgccccgt ccgtgctcca ggagcacgcc
    26161 cacgagtact tctcgatgga cccggaggtc gagagcccct acatgctgct ggttgccgac
    26221 gtgcgccccg acaagcgggt gaacgacgac gggcggcgcg ccggcctgcg ggggatggaa
    26281 ctgctcaagg cgaagcgctc ggtcgttccc gccatcacgc acgtggacta ctcggcccgc
    26341 gtgcagacgg tcgagcgcga ccgccacgcc tcgtaccacc ggctgatcag ccggttccac
    26401 gaggcgaccg gctgcccggt cgtcatcaac acgagcttca acgtgcgcgg cgagccgatc
    26461 gtctgctcgc ccgaggatgc cctgaactgc ttcctgaaca ccggcatgga cgccctggtc
    26521 atggagcact tcctcctgct caaggcggag cagcccgcgt cgctgctcgg caaccaggag
    26581 gagtacctga aggccttcaa gctcgactag caggcggctg accgcgatga actttcgaga
    26641 cctcaacctc cgacccaacg accgcgagct ccggcagttc tcggccctgt ggctcgtcgg
    26701 tttcggcctg ctcggcgcga tcgtggcctg gcgcgccggc gccttcgcct cgggcatccc
    26761 tcccggctgg cacgcgccgt ggcgcctgcc gatcgccctc tgggggatcg cggcgatggg
    26821 ggccgccgtc ggcctggccg tcccccgcgc gatccggccg gtctacgtcg cctggatggt
    26881 ggccgcgttc cccatcgggt gggccgtcag ccagctgttg ctcgcgctgg tctactacgt
    26941 gttgttcacc gcgttcggcc tggtgtttcg gatcgccggc cgcgacgccc ttggccggtc
    27001 gttcgatcgc ggcgcgtcga gctactgggt cgcgcgcaag ccggcaagca gcctcgagcg
    27061 gtacttcagg cagttctgag aggaaccggg atggtggatc gcgactccgt cgatcgcttc
    27121 gagaaggcgg ccgccgcgcc gcgcgcgtcg ctggccgccg agctgtggca gtttctcgcg
    27181 cacaacaaga agtggtggct gctgccgatc gtgatcgtgt tcgcgctgct gggggcgttg
    27241 atgctgcttg gcggcaccgc ggctgcgccg ttcatctaca cgctgttcta gggttccgtc
    27301 ggcggatctc cttgcacggc cctcgaccac gcgcaggccc tcggcctgtt cctgcacttc
    27361 aagctgcagg agaccgagaa cgacgacact atccgcgagg atcgtggccc cgccccgacg
    27421 gccctcgacc agggagagct gggcgtcgag cggaagctct acctgcgcga gctggtcgcg
    27481 cgattcggtt acgagctggc gctcaactgg aacctcggcg aggagaacac gcagacaccg
    27541 gatcagcagc gtgcgatggc gcgcttcatc cgtgagaccg acccatatcc ccacccgatc
    27601 gtcatccaca cctaccccca tctgcaggac aaggtctacg ttcccctgct cggcgcccag
    27661 tcggggttga gtggcatttc gctgcagaac cactggcgca ccgcccacca gcgcgtcctc
    27721 aagtggatca ccgagtcgga gaaggctggc aggccctggg tggtcgccaa cgacgagcag
    27781 aacccgcatt tcacaggggt gccgcccgat ccaggctacg agggacacga cggcgtggcg
    27841 cgtcccggca agatctacgt cgtctacctg cccgacggcg gcacgaccgc gctcgatctc
    27901 ggcccggcga aggcggagta cagcgtccgg tggtacgatc cgaggaacgg cggtccgctt
    27961 ctgccgggca gcgtgacctc ggtcacgggg cccggtgttg cgacgctcgg ccagccgcct
    28021 cgcgatcccg gcgacgactg ggtgatcctg gtcaggcggc gcggtgagga cacggagtga
    28081 ctcacatgaa gcacgctcga tcgatgctgc tggtcttgct ggttggggcg gtgggggcgg
    28141 gcgccgccgc ggcgcagggc gacgccgaca ggaaggagtg ggttccgatc ttcaacggga
    28201 agaacctcga cggctgggat gtgaagatca ccggccacga cctgaacgac aacgccggca
    28261 acaccttccg ggtcgagaac ggggtgctga agaccgccta cgatgcctac ggtcagtcgt
    28321 tcggcaaccg cttcggccac ctcttctacc gtcagaagct ctcccactac gtcgtggcgg
    28381 tggagtaccg cttcgtcggc gagcaggcgc ccggcgcgcc cgactgggcg ttccgcaaca
    28441 gcggcgtcat ggtgcactcc caatcggccc gcagcatgac gaaggggcag gacttcccgg
    28501 tcagcatcga ggtgcagctg ctcggcgggc cggagacagg cgagcgcacg accgcgaacc
    28561 tgtgcacccc cggcaccaac gtcgagatgg acggcaagct ggtgacccag cactgcgtca
    28621 actcgacctc caggacgtat cgcggcgacc agtgggtgcg cgtcgaggtc gaagtgctcg
    28681 gcggcgcgtc gatcaggcac atcgtcgagg ggcagacggt gctcgcgtac cagaagcccc
    28741 aggccggcgg cggcaacgtg accagcgccg acccttccgt ggtaaaagac ggggccctgc
    28801 tcgaggaggg gtacattgcc cttcagagcg agagtcaccc gatcgagttc cggaaggtgg
    28861 agctgctgaa cctcgttggc tgcaccgacc cgaaggccgc gaacttcaag agctactacg
    28921 tgaagtccga caacgccgcg tgccggtacc ggtaaggcca ccgcacagct gagcgtcccc
    28981 tccggggtcg ggagtaatta gaagtcgtgc cggatttccg atttccaatt actcccgacc
    29041 ccaatccttc cgtccgtggc acggccattg ctgcgacaga gcgcaagcgc gtcgtgaggg
    29101 ccggaggacg gtatgtggag ctatttcgcc aagggcggcc ccgtgatgtg gccgcttctg
    29161 gttctgtcga ttctcgccgt cgtggtgatc gtgtggcggt ggctcgcgct tcgcgaggcc
    29221 acgaggaacg cggcgtcgct gatggcggcc ctgaaggagc ggctgctcgc gcgcgatccg
    29281 atgggtgcgg tggcggcgtg cgatgctcac ccgggggccg tggcaagcat cgtccgcgcc
    29341 ggcctgctgc gcctcgggcg ccccaaggac gagatcgagc tggggctgca ggatgcgtcg
    29401 gcgcacgagc tcatggcgct cgagcgcggc ttgcccgtgc tcgccacagt cgcgatggtc
    29461 gcgccccttc tcggcttcct cgggaccgtc acagggatga tcaactcctt cgacgcgctg
    29521 gcggccgtgg gactgaacaa ccccgcggcc gtcgcatccg gcatttccga ggcgctgatc
    29581 accacggcgg ccggcctcat gatcgccatc cccgtgcaga tggcctacaa ctacttcgtg
    29641 gtgcgggtga acgtcacggt ccgcgacatg gaggcggctg ccaacctcgt gctcgaggcg
    29701 ctgaccgatg tccgcttgag cgagaccggc ggcgagcggg cggctgcgcc gcgtctcgcc
    29761 gttccgaacc ccaacccggc ggcggcgcgg taggaggagg gccgcatgcg cctccacagg
    29821 ccgcgcacga cgctgcgggg ggtgatcccg acggccacga tggccgacat cgccttcctg
    29881 ctgatcatct tcttcatggt cacgacggca tacagcctcg atcggaccgc gctcgagctg
    29941 ccccagaccg ccgagcagca gcaggcgccg aaaggggccg cgctggtcgt gattgcgccc
    30001 gacgacacgt tccgcttctc ggccggcgag gaggatgcca agctcgttga agggctggaa
    30061 gggctgggga ccgctgtccg gcaggtgact gccgccaaca ggcttcagcc gttcagcatc
    30121 aaggcggaca ggcgggtgaa gtaccgcacc atcgacgccg tgctggagca gttgcggcag
    30181 gcgggggccg agaacatcac gctgctgtcg cgcggggaga acgagcgatg agggtcccgc
    30241 gccgcgaggt gccgtcggag attccaaccg cgtcgatggc ggacgtggcc tttctcctga
    30301 tcatcttctt cctggtcgct tccttctaca gcgtgacgcg gggcgtgcag ttctcgctcc
    30361 cgcgccacga cgaggcgacc gtcaacgtcg agcccgaggc gtcggtgctc gtcgagatcg
    30421 cgcccgacgc ctcgctgcgg gtggacgggc gttcgatgcc gctcgcccac ctgcccggct
    30481 acctgcggcc caggctggtg gcgaacccgg cgaagccggt gatcatccgg accgacctgg
    30541 acgcgtcgta ctcggcgatg accgacgtgt tcgacgaact ccgccaggcc ggtgtccgca
    30601 acgtcgtcat ccctacgcag gccgagcttg cgttgtacgg gctggaggga cagcggtgac
    30661 cgaccaaccg caggctcgcc cgcccgaccg ccgggaggga cgcgcccgcg aggagctggc
    30721 gctggccgag cgctggctga ggagggaccc gcgcgaccga aagtgggtgg cccggggtgt
    30781 cgccgtcgcg ctgttgctgc acgcgggtgt cctgtttgcg cgcatgcctg gctggggggc
    30841 cgagccgaag cgggtggagc cgccgcagga gcaggccatg caggtgcagt tcct
//
LOCUS       DAOPQX010000003        30661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_1933_length_30661_cov_4.901849, whole genome shotgun sequence.
ACCESSION   DAOPQX010000003 DAOPQX010000000
VERSION     DAOPQX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 30661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30661
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1933_length_30661_cov_4.901849"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /gene="pnp"
                     /locus_tag="PKK95_00290"
     CDS             complement(<1..1499)
                     /gene="pnp"
                     /locus_tag="PKK95_00290"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOC16670.1"
                     /translation="MHKRELPVGRGVLSFETGKLAKQANGSVVVRFGDSVVLVTACYA
                     AAEREGIDFLPLTVDYREYTYASGRIPGGFFKREGKPSEKEVLTSRVIDRPMRPLFPP
                     GWHHETQIIALVLSADADNDPDVLAINGASAAVALSEIPFQRTIGAVRVALVDEEFLL
                     NPTYAERSRARLDIVVAGSREGLVMVEAGAKGVTEAQVVEALEVAHDAIRQIVDTIDR
                     MAQEAGKPKVEAPAREINRDFYREVEEKVWLPLGEAMRIQDKAENYEAVEQILEDLVA
                     SIPEEAVERRIEAKAIFKELKEKVLRDAVLEKGRRLDGRAFDEVRRIWIEVGVLPRVH
                     GSAVFTRGETQALVTATLGTAEDQQKVEMVSGEHYKRFMLHYNFPPFSVGEVAFLRGP
                     GRREVGHGALAERSLLPVLPTEEKFPYTVRVVSDILESNGSSSMASVCGGSLAMMDAG
                     VPLASPVAGVAMGLVMDESTGKYAVLTDIAGAEDHYGDMDFKVAGTTEG"
     gene            complement(1681..1950)
                     /gene="rpsO"
                     /locus_tag="PKK95_00295"
     CDS             complement(1681..1950)
                     /gene="rpsO"
                     /locus_tag="PKK95_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOC16671.1"
                     /translation="MALTKDRKTEIIDTYKTHDGDTGSPEVQVAILSERITYLTEHFK
                     VHGKDHHSRRGLLQLVGQRRRLLDYLKRKDTDRYAGLIRRLGIRK"
     gene            complement(2040..2939)
                     /gene="truB"
                     /locus_tag="PKK95_00300"
     CDS             complement(2040..2939)
                     /gene="truB"
                     /locus_tag="PKK95_00300"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOC16672.1"
                     /translation="MDGVLVVDKPEGPTSHDVVAVARRALGVRQVGHAGTLDPMATGV
                     LVLMVGSATRLARFLSSQPKAYDATILLGVETDSGDRTGRVISRAAPEASWPEPGTVR
                     VALGSLRGTFEQLPPAHSAKWVGGERAYTLARAGAEVPVRAVSVTLHDASIVAYEPPR
                     LRLAIRCSAGFYVRALARDLGRMLGCGGALEALRRTESADFTLDEAVPLDLLVSEPGA
                     ARERLIAMDRLLPGFPAVRLTAAGERKARHGNDLSAADLAGAAPPCPRGGMVRLLAAD
                     GRLVGLARPAAPPAVLHPAVVLQ"
     gene            complement(2943..3962)
                     /locus_tag="PKK95_00305"
     CDS             complement(2943..3962)
                     /locus_tag="PKK95_00305"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOC16673.1"
                     /translation="MKMATENRIEHERMAARIAAELRARQRFLITSHLKPDGDSIGSQ
                     MALAGALRALGKDVRVVNRDQAPSPLLTFPWVDQIEIADRAEGDFDAVVVMECGDLAR
                     TGVQGLERHFIVNIDHHPGNSMFGAINWFDAGAAACGEMVFDLVRALGVPMTPEIATH
                     IYVAILTDTGSFHYSSISPRTFDICRQTLEAGVDPVAVARNVFDSNNIGRLRLFGAVL
                     GSVELEDSGRLATIYLDRAMARAAGGTYDDTEGLINLPLTVKEIQAVAFFKEMSPAQY
                     RVSLRSKGAIDVGEVAKRFGGGGHKNASGCTVEGTLPEVRAQVIPLVVEAINKSAGGG
                     AEPSA"
     gene            complement(3934..4350)
                     /gene="rbfA"
                     /locus_tag="PKK95_00310"
     CDS             complement(3934..4350)
                     /gene="rbfA"
                     /locus_tag="PKK95_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOC16674.1"
                     /translation="MPQSHRPARVADQIREQLGEMLARDVHDPEIGFVTVTRVEVTPD
                     LQLARVFYTTMGGDAERRRTSRALGRATPFLRRQVGQRLGLRRVPVLEFRFDKSVEHQ
                     ERVERLIQEIHEQQRQQELERGNEPLPNEDGDREQD"
     gene            complement(4354..4620)
                     /locus_tag="PKK95_00315"
     CDS             complement(4354..4620)
                     /locus_tag="PKK95_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOC16675.1"
                     /translation="MTVELHLAGCRSLKDKRMVLRRVKDRLAKFNVAVAEVEYHDLWQ
                     RAALGVVTISTTEAHAQQELSRVVDEIERVEPGAISGTGVELLA"
     gene            complement(4646..6538)
                     /gene="infB"
                     /locus_tag="PKK95_00320"
     CDS             complement(4646..6538)
                     /gene="infB"
                     /locus_tag="PKK95_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOC16676.1"
                     /translation="MAPRPPARPGPGRPGARPAPGQRGRRDAPRVPAPTPSAPPPISR
                     TITLAEGMTVKDLADKLDVRTKDVLRKLLDKRIMMTINTTLDIETATMIAREFGADVQ
                     LRSFEEELLEVEGEVSRPEDLVPRAPVVTVMGHVDHGKTTLLDGIRETRVAEREAGGI
                     TQHIGAYHVQVNNRSVVFLDTPGHEAFTQMRARGARVTDIVILVVAADDGVMPQTREA
                     IDHARAAGVPIVVAINKIDKPDANAERVKRELADLGLVPEDWGGQTVMVEVSAKKRQN
                     LDLLLEMILLVTDMSELRANPKRPAVGTVLEAQLDKGRGPVATILVQDGTLHVGDTVI
                     AGPILGKVRALLDDRGRHVKAAVPSMPVEVLGLAALPQPGDSFQAINDAAKARQVALF
                     RQGQAKEKAMAGKATRLTLESLQQQIAEGDVKELPIVIKADVQGSAEVLADTLTKLSD
                     EKVKLRVIHAGVGAINESDVLLASASNAIIIGFSVRPDRNAAEVAEREHVDVRLHSII
                     YNVIDEMKKAMAGLLEPTFKEVRLGTAEVREVFRVPKVGAIAGCMVTDGVIRRAGDAQ
                     ARLLRDHVVVFEGKIGSLRRFKDDVSEVKSGFECGIGLERFNDIKVGDQIEVFVVERV
                     AAGAPA"
     gene            7547..7795
                     /locus_tag="PKK95_00325"
     CDS             7547..7795
                     /locus_tag="PKK95_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16677.1"
                     /translation="MLLALVSRRLGEHVAAGQRDAALPGHPLHELPRHDFLDGARRAL
                     DLDAVLAFQERHHFLARGAKQFSDFVNPNSGQRLPRKS"
     gene            complement(7807..9327)
                     /gene="nusA"
                     /locus_tag="PKK95_00330"
     CDS             complement(7807..9327)
                     /gene="nusA"
                     /locus_tag="PKK95_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOC16678.1"
                     /translation="MSTNPTQQTIETLAKEKGIEPEVIISAIEEAVKTASRKYYKTNE
                     DLQARFNRETGQVDLFALKQIVAEVTDPATQVSLAEAQELYGDEAEVGMEIEFPKSTE
                     VLGRIAAQTAKQVIFQKVREAERENIFAEYNQRIGEVMTGGVKRFENGDIVLELGRIE
                     AVLPRKEQSRAESYAPGDRVRAVIKAVNRSAKGPQIVLSRTDAALLVKLFEQEVPEIY
                     DGTVVIKGAVREAGDRAKVAVYSRERDVDPVGACVGMKGTRVQSIIRELRGEKIDIVE
                     WSDDSVAFVTNALSPAKVQRVSIVDDHERVMEVVVEDKQLSLAIGKKGQNVRLAAKLT
                     GWRIDIKSEEEKRREVEAQLERLEVPGEPGEAGEGEAPIPELVLPDIHDEIVASLRDA
                     GYQTAAQVAAASIETLMEIPGIDEGTAESVLVAATAQAEVERAEAERAEAERAEAEQA
                     EAEQAEAEAAAASGAEADAGAAAAEEVEAGAEPRDAGEPPAEGEDEPAERDGEQQA"
     gene            complement(9330..9791)
                     /locus_tag="PKK95_00335"
     CDS             complement(9330..9791)
                     /locus_tag="PKK95_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOC16679.1"
                     /translation="MAERVAATYGVEVFDLQFRREAPGWVLRVIIDRPEAAAAARPGA
                     PGESVGVDDCRHVSEDLGTLLDVEDAIEGAYTLEVSSPGLDRPLRGPADYERFRGRLA
                     QFVATETVDGQTHFRGRIQGLEEDCVVLVEGSRTHRIPVALVSRARLEVEF"
     gene            complement(9901..11259)
                     /gene="rimO"
                     /locus_tag="PKK95_00340"
     CDS             complement(9901..11259)
                     /gene="rimO"
                     /locus_tag="PKK95_00340"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOC16680.1"
                     /translation="MKIGLVSLGCPKNLVDSEVMLGLAEQAGHTITADAKEAEVLIVN
                     TCAFIDSAKQESIEAILEMAQHKKAGACRRLIVTGCLGERYREQLRRELPEVDEVLGT
                     NDVPAIVRAIGGSGAPAATPVRFHRSRPTYLYGAETPRRLTTPPHLAYVKIAEGCNYK
                     CAFCIIPRLRGPYRSRPRPSVVQEARDLAARGVRELLLVSQDTTFYGVDRGQRDELAA
                     LLRELDAVDGLRRIRLLYLYPTTITGPTLRAVRECERVCRYVDLPLQHASNSVLSRMR
                     RPGTRAGYERLIDRIRREIPGVTLRTSFIVGFPGETAEEFDELCRFVGEQRFDNVGVF
                     TYSHEEGTAAGRGEDDVPAAVKQQRRRHLMALQQEVARQAGRGHIGREVEVMVDGPSA
                     EHPLVLAGRTEGQAPEIDGVTYLTDADPEACRPGAIVRGAVEDARGYDLVVRPLSVLK
                     PA"
     gene            complement(11275..12570)
                     /gene="asnS"
                     /locus_tag="PKK95_00345"
     CDS             complement(11275..12570)
                     /gene="asnS"
                     /locus_tag="PKK95_00345"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOC16681.1"
                     /translation="MPIAYIEDIARHEGEQVTIRGWLHNRRSSGKIHFLTVRDGTGFI
                     QAVMSKGNVGPDAFTRADHLSQESAIVVTGQVRADSRAPGGFELDVAGLEVVGESHDY
                     PITPKEHGVDFLLDRRHLWIRSERQSAILRVRHEVVNAVRDFFNSRGFILVDTPIFTP
                     SAAEGTTTLFPVPYFDLGTAYLTQSGQLYNEANAAALGRVYCFGPTFRAEKSKTRRHL
                     TEFWMVEPEVAYATLDDTIELAEQLVVAVVERALDKRAQELKVLQRDTSRLERVKTPF
                     PRVSYDEAVKILHDKGTAFEWGNDLGGTDETVLSEQFDRPVAVHRYPAAVKAFYMEPD
                     PERPELALCVDVLAPEGYGEIIGGSQRIADPDLLLERIKQHDLPQEAFEWYLDIRRYG
                     SVPHSGFGMGIERVVSWICGLDHLREAIPYPRMIYRMYP"
     gene            complement(12579..13424)
                     /locus_tag="PKK95_00350"
     CDS             complement(12579..13424)
                     /locus_tag="PKK95_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC16682.1"
                     /translation="MRFGISTHLYHQERLSAAHLREMAEFGFTEIELFASLGHFDYRA
                     PAAHRDLAAWLRDAGLALHSVHAPIVEYVRGGEWGPPLSLAHADETARARAVAETLAA
                     LELARTVRFAYLVTHLGVPDEGGGTEALNNAAAADRSLREIGRAARDLGVRVALEIIP
                     NALSTPETLARLVEDEEAGETNHRFGICLDTGHAFMRGDLPDAIDAVSGELVTTHVHD
                     NRGASDDHLVPFEGHIDWPTAVMTLQKVGYEGVYLLELANTGTAREVLQKTVDARRRL
                     ADILA"
     gene            complement(13526..14581)
                     /locus_tag="PKK95_00355"
     CDS             complement(13526..14581)
                     /locus_tag="PKK95_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOC16683.1"
                     /translation="MTSNVTSDTVQVPEPVLHYLDRQGFAARSARVVPLTGDASDRRY
                     FRIIETDGSTSVLALHSAPFDYATLPFVNVTELLQQVPLPVPAIYDQAGDLGLLRLQD
                     LGDVTLQAHLGAVPAGEHAALYRRAVAMIELLQRRGDELASDRYVPYGMAFDVAKLTW
                     ELEFFVNHFVEAYRGAQLPYATREAIREEWKPIAEELSAEPRVLCHRDYHSRNLMAHQ
                     DELYVIDFQDARMGPDTYDLVSLLRDAYVDLTEEAVDDLIAFFLALRGQYDRHTDRSW
                     FGTYQADFRRRFDLMALQRNLKALGTFGYQTTTRGNPVYIQYMPRTLRHVRSNLGRHP
                     RFGRLQELLSTFVEELK"
     gene            complement(14581..15558)
                     /locus_tag="PKK95_00360"
     CDS             complement(14581..15558)
                     /locus_tag="PKK95_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOC16684.1"
                     /translation="MPALPPCVVLTAGLGTRLRPLTWLRAKPALPVAGVPLVNRILAW
                     LASAGARRAVLNLHHLPGTIRAAVAGGAPRGLEIRYSFEDPILGSAGGPKRAIALLDA
                     PRVLVVNGDTLTDVDLGALLRAHDESGALVTMAVVPNREPSRYGGVLAGDDGAVSGFA
                     PRGERSAGSWHFVGVQVVEAAVFERVPEDQPSESVGELYPRLVAERPGSVRAFRCDVG
                     FHDIGTPADYLATSIAMAGDDWSRLAGPRSAIGPGAEVSGSILWEDVTVGAGARVRGC
                     IVTDGVSVAPGAVFAGQVLVSERHAAAIPAGSGARRHEGLLTFQLGDQR"
     gene            complement(15560..16117)
                     /gene="cyaB"
                     /locus_tag="PKK95_00365"
     CDS             complement(15560..16117)
                     /gene="cyaB"
                     /locus_tag="PKK95_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOC16685.1"
                     /translation="MSGPIEREIKLRFEAADQAREAILRAGGTALRGRRLQEDCLLDT
                     DERVLGARQSVLRVRMEQGRSLLTFKGPVQPSRMKLREERETVAADGETLLYILEQLG
                     FRPWFRYQKYREEFGWRDAVIALDETPIGVFVEIEGSEQTIVEAAAAIGRSPADYIVH
                     SYRTLFVRECEARGVESGDMVFDGE"
     gene            complement(16181..17308)
                     /locus_tag="PKK95_00370"
     CDS             complement(16181..17308)
                     /locus_tag="PKK95_00370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOC16686.1"
                     /translation="MPSPLLALIADRIRAHGPMPVAEYVELALYHPGHGYYATAERRS
                     GRAGDFMTSVDVGPVFGELLGVQIAEMLDAVGGPAVDLVEAAAGSGRLSRDILDHLER
                     ASPRQYAAVRLHLVERSGTARASQASTLGRHAALLATSGPSLPRGVRGVIVANELLDA
                     LPPHLVVMRDGGLREVAIGLGGSALVPVEVEPTTPAIPEYLHRVGAQLRAGQYAEVNL
                     AAAEWIRQAASALEEGFLLLLDYGHEAGELYGDSHPAGTLTTFKRHAVEDRAGGPGWL
                     REPGGRDITSHVDFTGVRLAAESAGLETLGLVDQTYFLLALSEGIDPVEAHDERSLGR
                     RLALKTLLLPGGLGSTHKVLVFGRRVGRPRLKGLAAGGRLT"
     gene            complement(17383..18906)
                     /locus_tag="PKK95_00375"
     CDS             complement(17383..18906)
                     /locus_tag="PKK95_00375"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOC16687.1"
                     /translation="MSSRKITLFYALLIAVASLAVGMVLASRLDLSSESSAQTMNVPP
                     MNSAPLAGPVDATTFRTIAKNATGFVVNIRTRAKRRAPDMNDFFGGENPFDRFFGRPQ
                     PNRPRDQVVESAGTGFVISAGDALILTNNHVVEGATDIYVAFGEDQYDQEEFRAKLVG
                     RDQLTDSALIQLEEKPARPLVEAKFGDSAQMQPGDWVMAIGNPFGLAHTVTVGVISAT
                     KRPFFTAEQRSQDVLQTDAAINPGNSGGPLLNVRGEVIGINTAILANSRAEGNLGIGF
                     AIPINVVRNLLPDLRRGEVVRGRIGISMLEVPRDAVEELGLKERRGALVAQVPKDGPA
                     AKAGIQPGDVIIEVNGKPIADREELTRIVTGTKPGTTIPVTLVRDRKQMTLNVTIEKL
                     DLEAESGGSARGGTEEDTTAGFGLSLGNVTPSMARSLRLPEGMRGAVIMDIEQGSAAD
                     GSGLAQGDIILKVNNQEVTSAADASSLLQKIPSGGRALLLVWKSRQGQELFLTLKKE"
     gene            19146..20738
                     /locus_tag="PKK95_00380"
     CDS             19146..20738
                     /locus_tag="PKK95_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOC16688.1"
                     /translation="MASQSGNSASPAPDRTVWHDDGRQFGVLAQNMAGRYLAIAADIL
                     IGLLLLPYNVAHLGKSEYGLWMLVASVTTYASLLELGYGGALTKYVAKYRALRDPLAL
                     NQIVSTTLALYTGIGLVVYGAVTAIAFNLDHLFNVSAAQAATGRTVLLILGAYVGATF
                     AFRPFGTIPAGFQRTHWSSFVSIGTSSAVALVNVLVLAAGYGLVPLVAAVTVVRFVGL
                     FAYRAVAYRTFPGLQVRPSHVRLDRLKELTGFSVYMLLLDIGWKVSFSSPPVVIGAIL
                     GSVAVASWTVAERLAQAALRMTNQMNDALFAVIVDSDARRADRLRTILVQGTRMSLAS
                     VIPVGGGMALLAHPVVMAFVGPEFADAVSAVQALALMVIVRGGFATSLTMLKGAGGHR
                     AATVGSLIQAAATVVLGVLLVSPFGATGAAIGLAVPVAAVHVLLFVPAACRLAGVRPE
                     AVLWEGVWPALWPAGIAAAAFFGLRGWLGDGLWLGLLQLAGAVVVYVACFGVAIGSRD
                     RRFYTAKLRELVHWRSGVGVPA"
     gene            20771..21697
                     /locus_tag="PKK95_00385"
     CDS             20771..21697
                     /locus_tag="PKK95_00385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC16689.1"
                     /translation="MSISSAAIETGAGHAPSATHGAEVSRGERFEFGRNWARFLERLN
                     EERVGSAVESLKRPLGAESLEGKTFLDVGCGSGLFSLAARRLGARVHSFDYDPLSVAC
                     ARELRRRLGEADTSPGWRIEPGSALDRTYLEGLGQFDIVYSWGVLHHTGRMWDALELA
                     GRAVRPGGQLYIALYNDQGSKTVRWRRVKRLYNRLPRAVQPAFAALAVAPDEGKAALR
                     ALLSGRPGEYLRTWTRYQARRGMSKWRDIVDWVGGHPYEAAKVDEVFSFYRARGFALE
                     YLRCGGGLGCNEFVFRHLSPGPLALPPSPGTP"
     gene            complement(21698..22165)
                     /locus_tag="PKK95_00390"
     CDS             complement(21698..22165)
                     /locus_tag="PKK95_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16690.1"
                     /translation="MLRLNRVFLALALVAAVLAGTTFAAAQTAPATQSRARFVDTVRG
                     PADLQYLKPVIKVEKDGTIVTTFQVKNVSPKAIARLTIEEFWYDKAGNPVSGDKQFLK
                     KPLLAGETATIVLRTPKSPNMNRNSYKFSHANGDVKPKVVAKLEEPAAAQAGR"
     gene            complement(22258..22716)
                     /locus_tag="PKK95_00395"
     CDS             complement(22258..22716)
                     /locus_tag="PKK95_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16691.1"
                     /translation="MSRKSVVKSRCVLCGAKELVEPRGEERYCRDCWDKKIAVEEIVA
                     REFALKRYIRAHSAEKYLVYHSTIKRPCGQLIVIDDGYDLFLTITLYPNFSWDEPAYH
                     LEGDPEGRSFAEILVDVVATEVIEPWGGGKWHLEIFRSVSAEPEDWNGEM"
     gene            complement(22730..24871)
                     /gene="glgP"
                     /locus_tag="PKK95_00400"
     CDS             complement(22730..24871)
                     /gene="glgP"
                     /locus_tag="PKK95_00400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOC16692.1"
                     /translation="MERYPGLPPLPPRIARLEELATDLWWSWHYRARDVFRDLDYPLW
                     RLTAHNPVRMLRLIPRERLAEAAEDPAFLSLYDSVMSELDAARSARDTWWGLRFGGQA
                     NGGQVAYFSAEFALHQSLPLYAGGLGVLAGDTCKEASDLGIPLVGVGFMYPQGYFHQH
                     LTAEGWQQETYETLDWLDAPIEPAIRPDGTPCVVAVPIGDRSVLAAVWVVRGGRVKLY
                     LLDTNLPENAPWDRELSARLYGGNHDTRIQQEIVLGLGGVRALRAMGVEPAVWHLNEG
                     HAAFVVLQRIREFLERGYQFDQAVEEVRRTTVFTTHTPVPAGHDAFPFHLVETYLAGA
                     WGSLGGLRDNFLALGAYDNNSGNGMQFNMTALAIRSAGSVNAVSVLHRDVTQAMWSPL
                     WPELKREQPVRAITNGVHVATWVAAELSKLFEAYIAPDWRSRHDDPALWEKVHEIPDA
                     ELWQVRQQLRSYLFAFIRERARNRWTNESVSPGQVVAAGTLLNPDALTIGFARRFTAY
                     KRPELLFHDPERLRRILSKFRQPLQFVFAGKSHPADDNGKHHLQRIYKRAMDNGFGGR
                     VAFVDDYDLHVAHFLVQGCDVWMNTPRKPLEASGTSGMKAAINGVPHCSIGDGWWAEG
                     YAGSNGWLVDGGAPEGDHGAADAADADALYRILEEQIVPTFYDRDAAGVPHAWLRVVK
                     ESIRTVMPRFSARRMVKDYVESMYASAFERK"
     gene            25016..25852
                     /locus_tag="PKK95_00405"
     CDS             25016..25852
                     /locus_tag="PKK95_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOC16693.1"
                     /translation="MSSEQLFRDVWAALVLSALPWMAAAARAQEPPSRELEHLNWMEV
                     RELVPARIRTVLLPLGTLEGHGVAVNGSDTIAPLAMARAIAPKVNALIAPAVPYGITG
                     SLEAYPGGLTIPDEAYRPYVRAILHGLARSRFKNVILLNGHGGNTAALNAVAAEVGRE
                     AGIRTLVVNWWTFCSDVTLEVFGEDGGHAGENENAYVMAVDPSNVRRERYTGPELAIA
                     NPAPGAWSAYPSPASILLYKPGEGLPRFDAIKAKEYFDKVNAKVAALVEDIVGRWDRA
                     GI"
     gene            25946..27058
                     /locus_tag="PKK95_00410"
     CDS             25946..27058
                     /locus_tag="PKK95_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HOC16694.1"
                     /translation="MSGKMKRREFVRAGAAAVAAAAPRAVFGQAPAVSTRAVRPVVIS
                     SANGNRARNGGQQTCVERAFTMMAGGSDVLDALIAGVNIVELDPADTSVGYGGLPNAD
                     GVVQLDSCCMHGPRKRAGGVAAIEGVKTPSLVARAVMDATDHHLIVGEGAREFARKLG
                     FEILDDLNTETSRRMWLEWKRRTDPLHYLDPSDREMAIKKVSLSIMREFGADPEHYYG
                     TINCNGVNARGEVSGVTTTSGMAWKIPGRTGDSPILGAGLYVDNEVGAAGSTGRGEAN
                     LFNLSSYLVVENLRRGMHPKDAGMDALRRVSANTVEKRLLTPDGLPNFQLNFYVLNAR
                     GEYASVSMYQSTYAVCTESGPQTLASEALIPRTIPE"
     gene            27103..28269
                     /locus_tag="PKK95_00415"
     CDS             27103..28269
                     /locus_tag="PKK95_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOC16695.1"
                     /translation="MKRTIICLALLAPAIAALAQEGASRPRARDLGVGPGVFAPGPLN
                     AITDVAGVAVGHVTIVRGDEVRTGVTAILPHGGNLFQDKVAGAVFVGNAFGKLAGSTQ
                     VEELGTIETPIVLTNTLSVGVAIDAVVGYTLDQDGNGRVQSVNALVGETNDGGLNDIR
                     GRHVTREHVLEAIREARTGEVEEGSVGAGTGTRCYGWKGGIGTSSRQLPERYGSYRLG
                     VLVQTNFGGVLTIAGAPVGRELGRHAFAAPGGGPEAGADGSCMIVVATDAPLDARDLR
                     RLAARAVFGLARTGSSYSHGSGDFAVAFTTSLEMRTKAGETAPRARRVLPSEAVSPLF
                     QAALEATEEAVYNSLFRATTVTGRGGQRADALPIDRVREILRRYNPSPEAVPKT"
     gene            28507..28968
                     /locus_tag="PKK95_00420"
     CDS             28507..28968
                     /locus_tag="PKK95_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16696.1"
                     /translation="MSTRVVFLSVSLAMCLVAAPALAQSGGGGVRGGVSGDPDQFYFG
                     AHLDTGPLVEMLSFRPNVEIGLGDDLTTVAANFEFVYWIPIRRTAWNIYAGGGPALNV
                     YRFDRGRNDDTNTEPGFNVLIGLQHYRGVFAEFKLGLIDSPEIKFGLGFSF"
     gene            complement(29058..29351)
                     /locus_tag="PKK95_00425"
     CDS             complement(29058..29351)
                     /locus_tag="PKK95_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16697.1"
                     /translation="MVQQDRSEQEKAEGSRESLNVPAAADMESGAGQLSEPEKPAVPG
                     SENPRSDKGGSAQPGAGITNRPMQEERENQEAVPERGRTKGEEESEGEKEGRR"
     gene            29438..>30661
                     /locus_tag="PKK95_00430"
     CDS             29438..>30661
                     /locus_tag="PKK95_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOC16698.1"
                     /translation="MISHNSFDTEAELQLAGAPVLVYSLARLERAGFPRVGRLPYSLK
                     VLLENLLRHENGRFATPEDVQALASWDPARRGRREIAFTPARVLLQDFTGVPAVVDLA
                     AMRDGIVRLGGDPARVNPLQPVELVIDHSVQVDYHARNDAFHLNAELEFRRNRERYVF
                     LRWGQNAFSNFRVVPPDTGIVHQVNLEYLARVVFRDEVNGRASAFPDTLVGTDSHTTM
                     VNGLGVLGWGVGGIEAEACMLGQPISMLIPDVLGFRLVGRLAEGATATDLVLTITERL
                     RKHGVVGKFVEFYGAGLDSMTIADRATLSNMCPEFGATAAIFPIDDMTLDYLRLTGRD
                     PGQVALVEEYAKAQGMFRVAGAPEAEYSETLTLDLAEVEPSVSGPRRPQDRVGLKHAK
                     RSFAEALDRALADRRP"
BASE COUNT         4353 a        10779 c        10738 g         4791 t
ORIGIN      
        1 atcccctcgg tcgtgccggc caccttgaag tccatgtcgc cgtagtggtc ctcggcgccg
       61 gcgatgtcgg tgaggaccgc gtacttgccg gtcgactcgt ccatcaccag gcccatggcc
      121 acgccggcga cgggggaggc gagcgggacc ccggcgtcca tcatcgccag cgatccgccg
      181 cacaccgacg ccatcgagga cgagccgttc gactcgagga tgtccgacac cacgcggacg
      241 gtgtagggga acttctcctc ggttggaagc accggaagca gcgaccgctc ggcgagagcg
      301 ccgtggccga cctcgcgccg cccggggccg cgcaggaacg cgacctcgcc gaccgagaag
      361 ggcgggaagt tgtagtggag catgaagcgc ttgtagtgct cgccgctcac catctcgacc
      421 ttctgctggt cctcggcggt gccgagggtc gcggtgacca gcgcctgcgt ctcgcccctg
      481 gtgaagacgg ccgagccgtg gactcgcggc aggacgccga cctcgatcca gatgcgccgc
      541 acctcgtcga aggcccgccc gtcgagccgg cgccccttct cgagcaccgc gtcgcgcagc
      601 accttctcct tcagttcctt gaagatggcc ttcgcctcga tccggcgctc gaccgcctcc
      661 tccgggatcg acgcgacgag gtcctcgagg atctgctcga ccgcctcgta gttctcggcc
      721 ttgtcctgga tgcgcatcgc ctcgccgagc ggcagccaga ccttctcctc gacctcgcgg
      781 tagaagtcgc ggttgatctc cctggccggc gcctcgacct tcggcttccc ggcctcctgc
      841 gccatgcggt cgatcgtgtc cacaatctgg cggatcgcat cgtgcgcgac ctccagcgcc
      901 tcgacgacct gcgcctcggt cacgcccttg gcgccggcct cgaccatcac cagcccctcg
      961 cggctgccgg ccacgacgat gtccaggcgt gcccgcgagc gctcggcgta ggtcggattg
     1021 aggaggaact cctcgtccac cagtgcgacg cgcaccgcgc cgatcgtccg ctggaaggga
     1081 atctcggaga gcgcgacagc tgccgaggcc ccgttgatgg cgagcacgtc gggatcgttg
     1141 tccgcgtcgg ccgagaggac gagggcgatg atctgcgtct catggtgcca gccggggggg
     1201 aagagcgggc gcatcggccg gtcgatcacc cggctggtga gcacttcctt ctcgctcggc
     1261 ttcccctcgc gcttgaagaa cccgcccggg atccggcccg aggcgtacgt gtactcgcgg
     1321 tagtccaccg tcagcggcag gaagtcgatc ccctcccgct cggcggccgc gtagcacgcg
     1381 gtgaccagga cgaccgagtc gccgaaccgg accacgaccg agccgttggc ctgcttggcc
     1441 agcttgcccg tctcgaagga caggaccccg cgcccgacgg ggagttcacg cttgtgcatc
     1501 gtagttgcct cgctggaacg tcacggttgg cccgggagcc ccgggctggc gaaagcgacc
     1561 ccggcaggaa cgcggggcgg aaacccgatc ggaacggatg tcgcgggatg aaccgaaatt
     1621 cgtcccgatc ggatgctccg cggcgagggt cgatcggtcg gtcggcactg cgggaagcgg
     1681 ctacttccgg atgccgagac ggcggatcaa gccggcgtac cggtcggtat ccttccgctt
     1741 gaggtagtcc aacagccgcc ggcgctggcc aaccagctgc aggaggccgc ggcgggagtg
     1801 atggtccttg ccgtgcacct tgaagtgctc ggtcaggtaa gtgatgcgct cgctgaggat
     1861 ggcgacctgc acctcgggcg acccggtgtc cccgtcgtgg gtcttgtaag tgtcgatgat
     1921 ctcggtcttg cgatccttcg tcagtgccac ggctccgcac ctccacgcac ggcccagcca
     1981 cgccccgcgc gccaagccgc cgtatcaacc ggacggtttt cacgaacaga ctgtcaagtt
     2041 tactgcagga ctacggcggg atgcaaaacg gccggcggcg ccgccggccg cgccaggccg
     2101 accagccggc cgtcggcggc cagcaggcgg accattccgc cccgtgggca gggaggggcc
     2161 gcgcccgcga ggtcggctgc cgacaggtcg tttccgtgcc gcgccttgcg ctccccggcc
     2221 gccgtcaggc gcacggctgg gaacccgggc aggaggcgat ccatcgcgat gagacgctct
     2281 cgcgccgccc cgggctcgct cacgaggaga tcgagcggca ccgcctcatc cagcgtgaag
     2341 tcggcgctct cggtccggcg cagcgcctcg agcgccccgc cgcagccgag catccggccc
     2401 aggtcccgcg cgagcgcgcg cacgtagaag cccgccgagc agcgaatggc caggcgcagc
     2461 cgtggcggct cgtacgcgac gatcgaggcg tcgtgcagcg tgaccgacac agcacggacc
     2521 ggcacctcgg cgcccgcgcg ggccagggtg taggcccgct cgccgccgac ccacttcgcc
     2581 gagtgggcgg gcggcagctg ctcgaacgtg ccgcgcaggc ttcccagggc cacgcgcacc
     2641 gtgcccggtt cgggccagga ggcctcgggc gcggcacggc tgatgacccg gcccgtccgg
     2701 tcgcccgagt cggtctccac gccgagcagg atggtggcgt cgtaggcctt gggttgactg
     2761 gagagaaacc gggccagccg cgtggccgat ccgaccatca gcacgaggac gccggtggcc
     2821 atcgggtcga gggtcccggc gtggccgacc tggcgaacgc cgagtgcacg gcgcgcgacg
     2881 gcaaccacgt cgtgcgaggt gggaccttcc ggcttgtcga cgacgagtac cccgtccacg
     2941 ggtcaggcgg acggctcggc gccgccgccg gcgctcttgt tgatggcctc cacgaccagc
     3001 gggatcacct gcgcacggac ctcggggagg gtgccctcca ccgtgcagcc gctcgcgttc
     3061 ttgtgcccgc cgccgccgaa ccgcttggcg acctcgccca cgtcgattgc ccccttcgac
     3121 cggaggctca cgcggtactg ggccgggctc atctccttga agaaggcgac ggcctggatc
     3181 tccttgaccg tgagcgggag gttgatgagc ccctcggtgt cgtcgtaggt gccgccggcg
     3241 gccctggcca tggcgcggtc caggtagatg gtggccaggc gccccgagtc ctcgagctcg
     3301 accgacccga ggacggctcc gaacagccgc agccggccga tgttgttgct gtcgaagacg
     3361 ttcctcgcga cggccacggg gtcgacgccg gcctcgagcg tctggcggca gatgtcgaag
     3421 gtgcgcggcg agatgctcga gtagtggaac gagccggtgt cggtgaggat cgccacgtag
     3481 atgtgcgtgg cgatctcggg cgtcatcggc acgccgaggg cgcgcacgag atcgaagacc
     3541 atttcgccgc acgcggcggc gccggcgtcg aaccagttga tcgcgccgaa catcgagttg
     3601 ccggggtggt ggtcgatgtt gacgatgaag tgccgctcga gcccctgcac gccggttcgc
     3661 gcgaggtcgc cgcactccat cacgacgacg gcgtcgaagt cgccctcggc ccggtcggcg
     3721 atctcgatct gatccaccca ggggaaggtc agcagcggtg acggcgcctg gtcgcggttg
     3781 accacgcgca cgtccttccc cagggcgcgc agcgcgcccg ccagggccat ctgcgaaccg
     3841 atcgaatcgc cgtccggctt gaggtgcgag gtgatgagga aacgctggcg cgcccgcagc
     3901 tcggccgcga tgcgggccgc catgcgctcg tgctcaatcc tgttctcggt cgccatcttc
     3961 attcggtaga ggctcgttcc cgcgctccag ctcctgctgc cgttgctgct cgtggatttc
     4021 ctggatcagg cgctcgaccc gttcctggtg ctcgaccgac ttgtcgaacc ggaactcgag
     4081 caccggcacc cggcgaaggc cgaggcgctg gcccacctgg cggcgcagga agggggtggc
     4141 tcgcccgaga gcgcgcgagg tccgacggcg ctcggcgtcg ccgcccatcg tcgtgtagaa
     4201 cacccgcgcg agctggaggt ccggggtgac ctccacgcgg gtcacggtga cgaagccgat
     4261 ctccggatcg tgcacgtcgc gcgccagcat ctcgccgagc tgctcgcgaa tctggtcggc
     4321 gacccgggcg gggcggtggc tctggggcat ggctcaggcc agcagttcca cgccggtgcc
     4381 gctgatcgcc ccaggctcga cgcgctcgat ctcgtccacc acccgcgaca gctcctgctg
     4441 cgcgtgcgcc tcggtcgtgc tgatggtgac gacgccgagc gccgcccgct gccagaggtc
     4501 gtggtactcg acctcggcga ccgccacgtt gaacttcgca aggcggtcct tcacccggcg
     4561 cagcaccatc cgcttgtcct tcagcgaacg gcacccggcc aggtgaagct cgacggtcag
     4621 cagggcgacc ggcgtaacgg ccctcctacg cgggcgcgcc cgccgcgacg cgctcgacga
     4681 cgaagacctc gatctggtcg ccgaccttga tgtcgttgaa ccgctcgagg ccgatcccgc
     4741 actcgaagcc cgacttcacc tcggacacgt cgtccttgaa gcggcgcagc gacccgatct
     4801 tcccctcgaa cacgaccacg tggtcgcgga ggagccgggc ctgcgcgtcg cccgcgcggc
     4861 ggatcacgcc gtcggtgacc atgcacccgg caatcgcccc caccttcggg acgcggaaga
     4921 cctcgcggac ctcggccgtg cccagccgca cctccttgaa ggtcggctcg agcagccccg
     4981 ccatcgcctt cttcatctcg tcgatcacgt tgtagatgat cgagtggagg cggacgtcca
     5041 cgtgctcgcg ctcggccacc tcggcggcgt tgcggtcggg gcggacgctg aagccgatga
     5101 tgatcgcgtt cgaggccgag gcgagcagca cgtccgactc gttgatcgcg ccgacgcccg
     5161 cgtggatcac ccgcagcttc accttctcgt ccgacagctt ggtcagcgtg tcggccagca
     5221 cctcggccga cccctgcacg tcggccttga tgacgatcgg cagctccttg acgtcgccct
     5281 cggcaatctg ctgctgcagc gactcgagag tgaggcgcgt ggccttgccg gccatcgcct
     5341 tctccttggc ctgcccctgg cggaacagcg cgacctggcg ggccttggcg gcgtcgttga
     5401 tcgcctggaa cgagtcgccg ggctgcggca gcgccgcgag ccccagcacc tcgaccggca
     5461 tcgacggcac cgccgccttc acgtggcggc cgcggtcgtc gagcagcgcc ctcaccttgc
     5521 cgaggatcgg gcccgcgatc acggtgtctc cgacatgtag cgtcccgtcc tgcaccagga
     5581 tcgtcgcgac cgggccgcgc cccttgtcca gctgcgcctc gagcaccgtc ccgacggccg
     5641 gccgcttggg gttcgcccgc agctcgctca tgtccgtcac gagcaaaatc atctcgagca
     5701 ggagatcgag gttctgccgc ttcttggccg agacctccac catgacggtc tggccgcccc
     5761 agtcctcggg caccagcccg agatcggcca gctcgcgctt gacccgctcc gcgttggcgt
     5821 cgggcttgtc gatcttgttg atggccacga cgatcgggac gcccgccgcg cgcgcgtggt
     5881 cgatggcctc gcgcgtctgc ggcatcaccc cgtcgtcggc ggccacgacc aggatgacga
     5941 tgtcggtgac ccgggcgccg cgggcgcgca tctgggtgaa ggcctcgtgg ccgggcgtgt
     6001 cgaggaagac cacgctgcgg ttgttcacct gtacgtggta ggcgccgatg tgctgcgtaa
     6061 tgccgcccgc ctcgcgctcg gccacgcgcg tctcgcggat gccgtcgagc agcgtcgtct
     6121 tgccgtgatc gacgtggccc atcaccgtga ccacgggggc gcggggcacg aggtcttccg
     6181 gacggctgac ctcgccttcc acctccagca actcctcctc gaagctccgg agctggacgt
     6241 cggcgccgaa ctcgcgcgcg atcatcgtcg ccgtctcgat gtcgagcgtg gtgttgatcg
     6301 tcatcatgat ccgcttgtcg aggagcttcc gcaggacgtc cttcgtcctg acgtccagct
     6361 tgtcggcgag gtccttgacc gtcatcccct cggcgagcgt gatcgtgcgg ctgatcggcg
     6421 gcggtgccga cggcgtgggc gccgggacgc gcggcgcatc gcgacggccg cgctggccgg
     6481 gcgccggacg ggcgccgggc ctgccgggcc cgggccgcgc cggcggacgc ggggccattc
     6541 cgggccgtgc gggctggccg ggcagcggac gggggccgcc catcgcggga cgcggggcaa
     6601 cgcccgggcg agccgccatc ggcggacgaa cgcctggcgc cccgggcgcg gggcgcgcag
     6661 tgtccggaat tccggggcgc atccccgggc ggccggcctg gccggccgca cctggcgcct
     6721 ggcgtggagc cggcggtgtc ggcggcctca ggggctgcgg cgggacggtc gtccgaggct
     6781 gcagccgcgg tgggggcggc gggggagtct cttccacccg cagacgcatc tgtggcggca
     6841 ccacacgacc cctcgggggc atcaccggcc gcggcgtggg agcaggcggt ggggaagcgg
     6901 gcctgggcgt cgcaggcgca gcgggccttt cggcctgggc acgcccggca gcaggctgcg
     6961 cggcagcagg cggcacggga gcgggctgcg cggcagcagg ctgcgcggga ggggcctggg
     7021 ccggaacggc cggcgccggt ggagccggtg ccggacgaag cgtcggcggc ctgagtgccg
     7081 ggccgcccgg agcgggcagc gcctgtggcg tcgctggctg cggccgcttc gcctcctcgg
     7141 agggcgccgg cgcggcggct tcagccaccg gtttctcggc atgggccggc gcgggcggct
     7201 cggcggcctg cggggcggcg gccactgggg ctggcggctg gggctctacc gggggcggcg
     7261 gcgcaggcgg cgtctcctcg agacgcagcg tcggcagcgg caaggtggtc ggctttgccg
     7321 gagcttctgc ggcggcgggt gcggccggcg gcgcgggctc cggggctggg gccggcggcg
     7381 gcgcagcctc gacctccggc gccggcggtg cttcggcagc gggtgcgggc ggcgcgggcg
     7441 cctcggcccc gggctcgacc gggcgtggac ggatggtctt caccagccgt ggcggcggca
     7501 gcgccggcgc ggctggcctg ggcggttccg gcttcttggc ggcgccgtgc ttcttgccct
     7561 tgtgagccgg cgtctcggcg aacatgtcgc cgccgggcaa cgcgatgccg cgctgccggg
     7621 ccatccgctc cacgaactgc cgcgccacga cttcctcgat ggtgctcgac gcgctcttga
     7681 cctcgatgcc gtgctcgcgt ttcaggagcg ccaccacttc ctggctcgtg gtgccaagca
     7741 gttcagcgac tttgtaaatc cgaacagtgg ccaacggtta cctcgaaaat cctgattgac
     7801 tcgccctcac gcctgctgct cgccgtcccg ttccgccggt tcgtcctcgc cctccgccgg
     7861 cggctcgcct gcatcccgcg gctcggcgcc cgcctcgacc tcttcggctg cggcggcgcc
     7921 ggcgtccgct tcggccccgc ttgccgccgc tgcctcggcc tccgcctgct cggcctccgc
     7981 ttgctcggcc tcggcccgct cggcctcggc gcgctcggcc tcggcgcgct cgacttcggc
     8041 ctgcgccgtg gcggccacga ggacgctctc ggcggtaccc tcgtcgatgc cggggatctc
     8101 catcagcgtc tcgatcgacg cggcggcaac ctgcgcagcc gtctggtagc cggcgtcgcg
     8161 gaggctggcc acgatttcgt cgtggatgtc cggcagcacg agctcgggga tgggcgcctc
     8221 gccctcgccc gcctcacccg gctcgccagg cacctcgagg cgctcgagct gcgcctcgac
     8281 ctcgcggcgc ttctcctcct cgctcttgat gtcgattcgc cagccggtca gcttcgcggc
     8341 gaggcggacg ttctggccct tcttgccgat cgcgagcgac agctgcttgt cctcgacgac
     8401 cacctccatc acgcgctcgt ggtcatccac gatcgagacg cgctgcacct tggcggggct
     8461 cagcgcgttg gtgacgaagg cgacggagtc atcggaccac tcgacgatgt cgatcttctc
     8521 gccgcggagc tcgcggatga tcgactgcac gcgcgtaccc ttcatgccga cgcaggcgcc
     8581 caccgggtcg acgtcgcgct cgcgcgagta cacggcgacc ttggcgcggt cgcccgcctc
     8641 ccggacggcc cccttgatca cgaccgtgcc gtcgtaaatc tccgggacct cctgctcgaa
     8701 gagcttgacg agcagggcgg cgtcggtgcg cgaaaggacg atctgcggcc ccttcgcgct
     8761 gcggttcacc gccttgatca cggcccggac gcggtcgccg ggggcgtagc tctcggcgcg
     8821 ggactgctcc ttgcgtggga ggactgcctc gatccgcccc agctcgagca cgatgtcgcc
     8881 gttctcgaac cgcttgacgc cgccggtcat cacctcgccg atccgctgat tgtactccgc
     8941 gaagatgttc tcgcgctcgg cctcgcgaac cttctggaaa atgacctgct tggccgtctg
     9001 ggcggcgatg cgcccgagga cctcggtcga cttcgggaac tcgatctcca tcccgacctc
     9061 ggcctcgtca ccgtagagct cctgcgcctc ggcaagggag acctgggtgg cggggtcggt
     9121 gacctccgcg acgatctgct tcaacgcgaa caggtcgacc tggccggtct cgcggttgaa
     9181 gcgggcctgc aggtcctcgt tggtcttgta atacttgcgc gaggcggtct tcacggcctc
     9241 ctcgatggcc gagatgatga cctcgggctc gatgcccttt tccttggcca gcgtttcgat
     9301 cgtctgctgc gttgggttcg tgctcatact cagaactcga cttcgagccg ggcgcgggac
     9361 accagcgcga ccgggatgcg atgggtccgc gatccctcca ccagcaccac gcagtcctcc
     9421 tcgagcccct ggatccgtcc ccggaagtgc gtctgcccgt ccactgtctc ggttgccacg
     9481 aactgcgcga ggcgcccgcg gaagcgctcg taatcggccg gtccgcggag cgggcggtcg
     9541 agcccgggcg agctgacctc gagcgtgtac gcgccctcga tcgcgtcttc gacgtccagc
     9601 agcgtgccga gatcctcgct cacgtggcgg cagtcgtcga cgccgaccga ctcgccggga
     9661 gcgccgggcc gcgccgcggc ggccgcctcg ggccgatcga tgatgacccg cagcacccag
     9721 cccggcgcct cgcggcggaa ctgcagatcg aagacctcga ccccgtaggt cgccgccacg
     9781 cgttcggcca tccccctgac tcgcgccaga gtgtccactc gttctacccc agaaacacga
     9841 aaagtgggca ccggcccact tcgtttgcca cagcgtagac acccgaagca gttcagtata
     9901 tcacgcaggt ttcaacaccg acaacggacg gaccactaga tcataccccc gggcgtcctc
     9961 cacggccccg cgcacgatcg ctcccgggcg gcaggcctcg gggtcggcgt cagtgaggta
    10021 ggtcaccccg tcgatctcgg gcgcctggcc ctcggtccgg cccgccagga ccaggggatg
    10081 ctcggccgac ggcccgtcca ccatcacctc aacctcgcgg ccgatgtgcc cccgccccgc
    10141 ttgccgcgcc acttcctgct gcagggccat caggtggcgc cggcgctgct gcttcaccgc
    10201 cgccggcacg tcgtcctcgc cccggcccgc ggcggttcct tcctcgtgcg agtaggtgaa
    10261 gacgccgacg ttgtcgaacc gctgctcgcc gacgaagcgg cagagttcgt cgaactcctc
    10321 ggccgtctcg cccgggaacc cgacgatgaa gctcgtgcgc agcgtgacgc cggggatctc
    10381 gcggcggatg cgatcgatga gccgctcgta gccggcgcgc gtgccgggcc ggcgcatgcg
    10441 ggagaggacg ctgttggagg cgtgctgcag cggcaggtcc acgtagcggc agacccgctc
    10501 gcactcgcgg acggcgcgca gcgtcggtcc ggtgatcgtc gtggggtaga ggtagaggag
    10561 ccggatgcgc cgcaggccgt ccaccgcgtc gagctcgcgc agcagcgcgg ccagctcgtc
    10621 ccgctggccg cggtccacac cgtaaaaggt ggtgtcctgc gagacgagga gcagctcgcg
    10681 gacgccccgg gcggcgaggt cgcgcgcttc ctgcaccacc gacggccgcg gccggctgcg
    10741 atacgggccc cgcagccgcg ggatgatgca gaaggcgcac ttgtagttgc atccctccgc
    10801 gatcttgacg tacgccaggt gcggcggcgt cgtcaggcgg cggggcgtct cggccccgta
    10861 gaggtaggtc ggccgcgatc ggtggaagcg cacgggggtg gccgccgggg cgccgcttcc
    10921 gccaattgcc cggacgatcg ccggcacgtc gttggtgccg aggacctcgt ccacctcggg
    10981 cagctcgcgc cgcagctgct cgcggtaccg ctcgcccagg cagcccgtca cgatcagccg
    11041 gcggcacgcc cccgccttct tgtgctgcgc catctcgagg atggcctcaa tcgactcctg
    11101 cttggccgag tcgatgaagg cgcaggtgtt gacgatcagc acctccgcct ccttcgcgtc
    11161 ggcggtgatc gtgtggccgg cctgctcggc caggccgagc atgacctccg agtccaccag
    11221 gttcttcggg cagccgaggg aaaccaagcc tatcttcatg ggggtcacgg gatcctaggg
    11281 atacatccgg tagatcatcc tcgggtacgg gatcgcttcc cgcaggtggt cgaggccgca
    11341 gatccaggag accacccgct cgatgcccat cccgaacccg ctgtgcggca ccgagccgta
    11401 ccggcggatg tcgaggtacc actcgaaggc ttcctgcggc aggtcgtgct gcttgatccg
    11461 ctcgagcaac aggtcggggt cggcgatgcg ctggctgccg ccgatgatct cgccgtagcc
    11521 ttcgggggcg aggacgtcca cgcacagggc cagctccggc cgctcggggt cgggctccat
    11581 gtagaacgcc ttgaccgccg cggggtagcg gtggacggcc acggggcgat cgaactgctc
    11641 cgagagcacg gtctcgtcgg tgccgcccag gtcgttgccc cactcgaagg ccgtcccctt
    11701 gtcgtgcagg atcttcaccg cctcgtcgta ggagacgcgc gggaacggcg tcttcacgcg
    11761 ctcgaggcgg ctcgtgtcgc gctgcagcac cttcagctcc tgcgcccgct tgtcgagcgc
    11821 gcgctcgacc acggcgacca ccagctgctc ggccagctcg atggtgtcgt cgagcgtggc
    11881 gtacgcgacc tccggctcca ccatccagaa ctcggtgagg tggcgccgcg ttttcgactt
    11941 ctcggcgcgg aacgtcggac cgaagcagta cacccgcccg agcgccgcgg cgttggcctc
    12001 gttgtacagc tgcccgctct gcgtcaggta ggcggtccca aggtcgaaat agggcacggg
    12061 gaacagcgtc gtcgtcccct cggccgccga cggcgtgaag atcggcgtgt cgaccaggat
    12121 gaacccgcgg ctgttgaaga agtcgcgcac cgcgttcacc acctcgtggc gcacccggag
    12181 gatcgccgac tgccgttcgg accggatcca caggtggcgg cggtccagca ggaagtcgac
    12241 gccgtgctcc ttcggggtga tggggtagtc gtgcgactcg ccgaccactt cgaggcccgc
    12301 cacgtcgagc tcgaacccgc ccggcgcccg gctgtctgcg cgcacctggc cggtcaccac
    12361 gatcgccgac tcctgggaga gatggtcggc acgcgtgaac gcgtcgggcc cgacgttccc
    12421 cttcgacatc accgcctgga tgaagccggt gccgtcccga acggtgagga agtggatctt
    12481 cccgctggag cggcggttgt gcagccagcc ccggatggtg acctgctcac cttcgtgccg
    12541 ggcgatgtcc tcgatgtagg cgatcggcat agggtccttc aggcgagaat gtccgccagg
    12601 cgccggcggg cgtccacggt cttctgcagc acctcgcgcg cggtgccggt attggccagc
    12661 tcgagcaggt acaccccctc gtagcccacc ttctgcaggg tcatcaccgc ggtcggccag
    12721 tcgatgtgcc cctcgaacgg caccaggtgg tcgtcggagg cgccgcggtt gtcgtggaca
    12781 tgggtggtga ccagctcccc cgacaccgcg tcgatggcgt ccggcagatc gccgcgcatg
    12841 aacgcgtggc cggtgtcgag gcagatgccg aagcgatggt tcgtctcccc ggcctcctcg
    12901 tcctccacga gccgcgccag cgtctcgggg gtggacagcg cgttggggat gatttcgagc
    12961 gccacgcgga cgccgaggtc gcgggctgcc cgcccgattt ccctcaagct gcgatcggcc
    13021 gcggcggcgt tgttcaacgc ctcggtaccg cctccctcgt cggggacgcc gaggtgcgtg
    13081 acgaggtagg cgaaccgcac cgtgcgcgcc agctcgagcg cggccagcgt ctcggccacg
    13141 gcccgtgcgc gcgcggtctc gtcggcgtgt gcgagcgaga gcgggggacc ccactcgccg
    13201 ccccgcacgt actccacgat gggggcgtgc accgagtgca gcgccagacc ggcgtcgcgc
    13261 agccatgcgg caaggtcgcg gtgcgcggcc ggcgcacggt agtcgaagtg acccagcgag
    13321 gcgaacagct cgatctcggt gaacccgaac tcggccatct cccgcaagtg agccgccgac
    13381 agccgttcct ggtggtagag gtgagtcgag atgccgaatc gcaagggcgg aaggctcctc
    13441 tccgggggaa gacggaaacc ctcattctac cgaatctgcc ggcgcgcggg ccccaccacg
    13501 atcccggccg gcacggaggc cggcgctatt tcagctcctc cacgaaggtt gacagcagtt
    13561 cctggaggcg cccgaacctg gggtggcgcc cgaggttgct ccgcacgtgg cggagggtgc
    13621 ggggcatgta ctggatgtag accgggttgc cgcgcgtcgt ggtttggtac ccgaaggtcc
    13681 ccagggcctt caggttgcgc tgcagcgcca tgaggtcgaa ccgccgccgg aagtcggcct
    13741 ggtaggtgcc gaaccagctg cggtcggtgt gccgatcgta ttgccctcgg agggccagga
    13801 agaaggcgat caggtcgtcg acggcctcct cggtgaggtc cacgtacgcg tcgcgcagca
    13861 gcgacaccag gtcgtaggtg tcgggcccca tccgcgcatc ctggaagtcg atcacgtaca
    13921 gctcgtcctg gtgcgccatc aggttgcgac tgtggtagtc gcggtggcag agaacccgcg
    13981 gctcggcgga cagctcctcg gcgattggct tccattcctc gcgaatcgcc tcccgcgtcg
    14041 cgtacggcag ctgggcgccc ctgtaggctt cgacgaagtg attgacgaag aactccagct
    14101 cccaggtcag cttcgccacg tcgaaggcca tgccgtaggg cacgtaccgg tccgaggcca
    14161 gctcgtcccc gcgccgctgc agcagctcga tcatcgccac ggcgcggcgg tacagcgcgg
    14221 catgctcgcc ggccggcacc gccccgaggt gggcctgcag cgtgacgtcg ccaaggtcct
    14281 gcagtcgcag cagcccgagg tcgccagcct gatcgtagat ggccgggacc ggcagcggga
    14341 cctgctgcag cagctcggtg acgttcacga acggcagggt cgcgtagtcg aagggagccg
    14401 agtgcagtgc caggaccgac gtgctcccgt cggtctcgat gatgcggaag taccggcggt
    14461 cggaggcgtc gcctgtcagc ggcaccacgc gcgcggatcg cgccgcgaac ccctgccggt
    14521 cgagatagtg caggaccggc tccggcactt gtaccgtgtc gctcgtcaca ttcgacgtca
    14581 tcaccgttga tcccccaact ggaacgtcag cagaccctcg tgccggcggg cacccgagcc
    14641 ggctggaatg gcggccgcgt gccgctccga caccaggact tgtcccgcga aaaccgctcc
    14701 gggggcgacc gacaccccgt cggtgacgat gcacccgcgt acccgcgcgc cggcccccac
    14761 cgtgacatcc tcccacagga tcgacccgga gacctcggcc cccggcccga ttgccgatcg
    14821 cgggccggcg agccgcgacc agtcgtctcc cgccatcgcg atcgacgtgg ccaggtaatc
    14881 ggcgggcgtt ccgatgtcgt ggaagcccac gtcacaccga aacgcgcgga cgctccccgg
    14941 gcgctcggcg acgagccgcg ggtacagctc gccgaccgac tccgacggct ggtcttccgg
    15001 cacgcgttcg aacacggcgg cttcgaccac ttgcacgccc acgaagtgcc acgagccagc
    15061 cgaccgctcg ccccgcggcg cgaaccccga caccgcgccg tcgtcgccag ccagcacgcc
    15121 gccgtagcgc gagggctcgc ggttggggac gaccgccatc gtcaccagtg cgccggactc
    15181 gtcatgggcg cgaagcagcg cgcccagatc cacgtcggtc agcgtgtcgc cgttcaccac
    15241 gagcacgcgg ggcgcgtcga gcagcgcgat ggcccgcttc ggccctcccg ccgagccgag
    15301 gatgggatcc tcgaacgagt agcgaatctc gagcccccgc ggggcgccgc ccgctaccgc
    15361 ggcgcgaatg gtcccgggga ggtgatggag gttcagcacc gcccgtctgg cgccggccga
    15421 cgccagccag gcaaggatgc ggttcaccag cggcacgccg gccaccggca gcgccggttt
    15481 cgcgcgcagc caggtgagcg gacgcagccg cgtgccgagc ccggccgtca ggacgacaca
    15541 agggggaagc gccggcacgt cactccccgt cgaagaccat gtcgccgctt tccaccccgc
    15601 gggcctcgca ctcgcggacg aagagggttc tgtacgagtg gacgatgtag tcggccggcg
    15661 accgcccgat ggccgcggcg gcctccacga tcgtctgctc gctgccttcg atctccacga
    15721 agacgccgat cggcgtctcg tcgagggcga tgaccgcgtc gcgccacccg aactcctccc
    15781 ggtacttctg ataccggaac cacggcctga agccgagctg ctcgaggatg tagagcagcg
    15841 tctcgccgtc ggcggccacc gtctcgcgct cctcgcgcag cttcatgcgc gacggctgca
    15901 cggggccctt gaaggtgagc aggctgcgcc cctgctccat ccggacgcga aggaccgact
    15961 gccgcgctcc cagcacgcgc tcatcggtgt cgagcaggca gtcctcctgc aggcgccgtc
    16021 cacgcagcgc agtgccgccc gcacgcagga ttgcctcccg agcctggtcg gccgcctcga
    16081 accgcagctt gatctcacgc tcgatcgggc cgctcatcgc gtctccttcg tcaaaggtgg
    16141 gggcggaagc acgaaagaga aggtggaccg agcgccccgc tcatgtcaac cgcccgcctg
    16201 ccgcaaggcc cttcaggcgc ggccggccga cccggcggcc gaagaccagc accttgtggg
    16261 tgctgccaag cccgccgggc agcagcagcg tcttcaacgc caggcggcgt cccagcgacc
    16321 gctcgtcgtg ggcctcgacc ggatcaattc cctcggagag ggccaggagg aagtacgtct
    16381 ggtcgacgag gccgagcgtc tcgaggccag ccgactccgc ggccagccgc acgccggtga
    16441 agtcgacgtg cgaggtgatg tcccgccccc ccggctcgcg cagccacccg gggccgccgg
    16501 cccggtcctc gacggcgtga cgcttgaagg tcgtcagcgt accggccggg tggctgtcgc
    16561 cgtacagttc accggcctcg tgaccgtagt cgagcagcag caggaatcct tcctcgagcg
    16621 ccgaggcggc ctgccggatc cactccgccg ccgcgaggtt gacctcggca tactggcctg
    16681 cgcggagctg ggcgccgacc cggtgaaggt actcggggat ggcgggagtg gtgggctcga
    16741 cctcgaccgg aacgagcgcg gaccctccaa ggccgatcgc gacttcccgc agtccgccgt
    16801 ctcgcatcac gacgaggtgc ggaggcaggg cgtccagcag ctcgttcgca acgatgacgc
    16861 ctcggacgcc acgaggcagc gatgggccag acgtcgcgag gagggcggca tgccggccga
    16921 gtgtcgacgc ctggctggcg cgcgccgtcc cgcttcgctc gaccaggtgc agccgcacgg
    16981 cggcgtactg gcgcggcgat gcgcgctcga gatgatcgag gatgtcgcgc gagagccggc
    17041 cgctcccggc ggccgcctcc acgaggtcca ccgcgggacc gcccaccgcg tccagcatct
    17101 cggcgatctg cacccccagc agttccccga agaccggccc cacatcgacg ctcgtcatga
    17161 agtcgccggc ccggcccgag cggcgctcgg cggtcgcgta gtaaccatgg ccggggtgat
    17221 agagggccag ttcgacgtac tcggcaacgg gcatcgggcc gtgcgccctg atgcggtcgg
    17281 cgatgagggc gagcagcgga gagggcatga aagacgaagg ccgggcggaa cacgttcgtc
    17341 ccgcccggcc ccattatcag cccgcagcca gctgccggat gcctactcct tcttcagcgt
    17401 caggaacagc tcctggccct ggcggctctt ccacacgagc agcagcgccc ggccgcccga
    17461 cgggatcttc tggaggaggc tgctggcgtc ggccgccgag gtgacctcct ggttgttcac
    17521 cttcaggatg atgtccccct gggcgaggcc gctgccgtcc gcggcgctgc cctgctcgat
    17581 gtccatgatc accgcgcccc gcatcccctc gggcaggcgc agcgaccgcg ccatcgacgg
    17641 cgtgacgttg ccgagcgaca ggccgaagcc ggcggtggtg tcttcctccg tgccgccgcg
    17701 ggcggatccg ccgctctcgg cctcgaggtc gagcttttcg atggtgacgt tgagggtcat
    17761 ctgcttccgg tcgcgcacca gggtcaccgg gatcgtcgtt cccggcttgg tgcccgtgac
    17821 gatccgcgtc agctcttcgc ggtcggcgat cggcttgccg ttcacctcga tgatgacgtc
    17881 gcccggctgg atccccgcct tcgcggccgg gccgtccttg ggcacctgcg cgacgagggc
    17941 gccccgccgc tccttcagcc cgagctcctc gaccgcgtca cgcgggactt cgagcatgct
    18001 gatgccgatg cgtccccgga ccacctcgcc ccggcgcagg tcgggcagca ggttgcgcac
    18061 cacgttgatc gggatcgcga acccgatgcc gaggttcccc tcggcgcgcg agttcgccag
    18121 gatcgcggtg ttgatgccga tcacttcgcc gcgcacgttg agcagcggcc cccccgagtt
    18181 gccggggttg atggccgcgt cggtctgcag cacgtcctgc gaccgctgtt ccgcggtgaa
    18241 aaaggggcgc ttggtggccg agatcacccc gacggtgacg gtatgcgcga ggccgaacgg
    18301 gttgccgatg gccatcaccc agtcacccgg ctgcatctgc gccgagtcgc cgaacttcgc
    18361 ctcgacgagc ggccgggccg gcttctcctc gagctggatc agcgcgctgt cggtcagctg
    18421 gtcgcggccc accagcttgg cgcggaactc ctcctggtcg tactggtctt cgccgaacgc
    18481 cacgtagatg tcggtcgccc cttcgaccac gtggttgttg gtcaggatga gggcgtcgcc
    18541 cgcgctgatg acgaagccgg tcccggccga ctcgaccacc tggtcgcgcg gccgattggg
    18601 ctgcgggcgg ccgaagaagc ggtcgaacgg gttctcgccg ccgaagaagt cgttcatgtc
    18661 gggggcgcgg cgcttggcgc gcgtccggat gttgacgacg aaccctgtcg cgttcttggc
    18721 gatcgtgcgg aacgtggtcg cgtccacggg ccccgccagc ggcgcgctgt tcatcggcgg
    18781 cacgttcatc gtctgtgcgg acgactcgga ggagagatcg agccgcgagg cgagcaccat
    18841 gccgacggcg agcgacgcga cggcgatcag gagggcgtag aacagcgtga tcttgcggct
    18901 cgacatcgga gaaacctcca accagcggga catccacgca aaccgcacgc cgttgcgagg
    18961 cgcgagccgg atgtcccttc tgcagagtat accctcggcc cgagggcaca ggcggtcctc
    19021 tggtaggacg ctccggccgg ctcgagagtc ggtgccgggc cgttgctcca gccgatcgaa
    19081 gcccaagcca gcatctgagc ctcagccgtt ctcctggcat cactctcgca aggctgagcc
    19141 gcggcgtggc atctcaatca ggcaactcag caagcccggc acccgaccgc accgtctggc
    19201 acgacgacgg gcgccagttc ggtgtgctgg cccagaacat ggcgggccgg tatctcgcga
    19261 tcgcggccga catcctcatc ggtctgctgc tgctgcccta caatgtcgcc cacctcggca
    19321 agtcggaata cggcctctgg atgctggtcg cgtcggtgac cacctatgcc tcgctgctcg
    19381 agctcggcta tggcggtgcg ctgaccaagt acgtcgcaaa gtaccgagcc ctccgcgacc
    19441 cgctcgcgct gaaccagatt gtcagcacga cgctcgcgct ctacaccggt atcgggctgg
    19501 tcgtctacgg cgcggtcacc gcgattgcct tcaacctcga tcacctcttc aacgtcagcg
    19561 ccgcgcaggc ggcgacgggg cggacggtcc tgctgatcct cggggcctac gtgggcgcca
    19621 ccttcgcctt caggcccttc gggacgatcc cggcgggctt ccagcgtact cactggagca
    19681 gcttcgtcag catcggcacg agctcggccg ttgccctcgt caatgtcctg gtgctggcgg
    19741 ccggctacgg cctggtgccg ctggtcgctg cggtgaccgt cgtgcgcttc gtcggcctct
    19801 tcgcgtaccg cgcggtcgcc taccgcacgt ttcccggcct gcaggtgcgg ccgtcgcacg
    19861 tcaggctgga ccggctgaag gagctgaccg gcttcagcgt ctacatgctg ctgctcgaca
    19921 tcggctggaa ggtgagcttc agctcgccgc cggtcgtcat cggcgccatc ctcggctcgg
    19981 tcgccgtggc cagctggacc gttgccgagc ggctcgcgca ggcggcgctc cgcatgacca
    20041 accagatgaa cgacgcgctg ttcgcggtga tcgtcgacag cgacgccagg cgcgccgatc
    20101 gcctgcggac gatcctcgtg cagggcacgc ggatgtcgct ggccagcgtg atcccggtcg
    20161 gcggcgggat ggccctgctg gcgcaccccg tcgtgatggc cttcgtcggc cccgagttcg
    20221 ccgacgcggt gtcggcggtg caggcgctcg ccttgatggt gatcgtgcgc ggcggcttcg
    20281 ctacctcgct gacgatgctc aagggcgccg ggggccatcg ggcggcgacc gtgggatcgc
    20341 tgatccaagc cgcggcgacc gtcgtgctcg gggttctgct ggtttcgccg tttggcgcca
    20401 cgggtgcggc catcggcctg gccgtcccgg tggcggcggt ccacgtcttg ttgttcgtgc
    20461 ccgcggcgtg ccggcttgcc ggggtccgtc cggaggcggt gctctgggag ggcgtgtggc
    20521 cggcgctctg gccggcgggc atcgccgccg cggccttctt cgggctgcgt ggctggctgg
    20581 gtgacggcct ctggctgggc ctgctccaac tggcgggagc ggtggtcgtg tacgtcgcgt
    20641 gcttcggtgt ggcgatcggg tcgagggacc gccggttcta cacggcgaag ctgcgcgagc
    20701 tggtgcactg gcgctctggc gtcggcgtgc ccgcgtgaaa caggcgggca aggaaggatc
    20761 ggtgagggcg atgtcgattt cctccgcagc gatcgagacc ggcgcgggcc acgcgcccag
    20821 cgccacccac ggggccgaag tctctcgcgg cgagcggttc gagttcggcc gcaactgggc
    20881 gcggttcctc gagcggctca acgaggagcg cgtcggatcg gccgtcgaat cgctcaagcg
    20941 gccgctcgga gcggagagcc tcgaggggaa gaccttcctg gacgtcgggt gcggcagcgg
    21001 tctcttcagc cttgcggcgc ggcggctggg ggcccgagtc cactccttcg actacgaccc
    21061 gctctcggtg gcctgcgcga gggagctgag gcggcgcctc ggtgaggccg acacctcccc
    21121 cggctggcgc atcgagccgg gcagcgccct cgaccgcacg tacctggagg gcctcggcca
    21181 gttcgacatc gtctattcct ggggcgtgct gcaccacacg ggccggatgt gggacgccct
    21241 cgaactcgcc ggacgtgcgg tgcggcccgg cggccagctg tacatcgcgc tctacaacga
    21301 ccaggggagc aagacggtgc ggtggcgacg ggtgaagcgg ctctacaaca ggttgccccg
    21361 tgccgtgcaa ccggccttcg cagcgctggc ggtcgccccc gacgaaggca aggcggcgct
    21421 ccgcgccctc ctgtcggggc ggccgggaga gtatctgcgg acgtggacgc gctaccaggc
    21481 gcgccgtggc atgagcaagt ggcgggatat cgtggactgg gtcggagggc acccctacga
    21541 ggcggcgaag gtggacgagg tgttctcttt ctaccgcgcc cgcggctttg ccctcgagta
    21601 cttgcgctgc gggggcgggc tggggtgcaa cgaattcgtc ttccggcacc tcagcccggg
    21661 cccgctcgcg cttccgcctt cgcccggaac gccctgacta ccggcccgcc tgagcggcgg
    21721 ccggttcctc gagcttcgcc accaccttcg gcttcacgtc gccgttggcg tgggagaact
    21781 tgtagctgtt ccggttcatg ttcgggctct tcggcgtgcg caggacgatg gtcgccgtct
    21841 cgccggccag cagcggcttc ttgaggaact gcttgtcgcc cgacacgggg ttgccggcct
    21901 tgtcgtacca gaactcctcg atggtgaggc gggcaatcgc cttcggcgag acgttcttca
    21961 cctggaaggt cgtgacaatc gtcccgtcct tctcgacctt gatcaccggc ttcaggtact
    22021 ggagatccgc cggacccctg acggtgtcga cgaacctggc ccgcgactgg gtggcgggcg
    22081 cggtctgggc ggcggcgaag gtcgtgccgg ccagcacggc ggcgaccagg gctagcgcca
    22141 ggaatacgcg attgaggcgg agcatcgaaa cctcccgggg ggccagacaa tgaatgacgg
    22201 atgacggatg gatggcgcgg acgccgacga ctgccgatcc gaggcgatta tagagcgtta
    22261 catctcgccg ttccagtcct cgggctcggc cgaaacggac cggaagatct cgaggtgcca
    22321 cttgccgccg ccccacggct cgatgacctc ggtggcgacg acgtccacga ggatttcggc
    22381 gaaactgcgg ccctcggggt cgccctcgag gtggtaggcc ggctcgtccc aggagaagtt
    22441 ggggtacagc gtgatcgtga ggaagaggtc gtatccgtcg tcgatgacga tgagctggcc
    22501 gcaggggcgc ttgatggtgg agtggtagac gaggtacttc tcggcgctgt gggcccggat
    22561 gtagcgcttc agcgcgaact cgcgcgcgac gatctcctcg accgcgatct tcttgtccca
    22621 gcagtcgcgg cagtagcgct cttcgccccg gggctccacc agttccttgg cgccgcacag
    22681 cacgcaccgc gatttcacga ccgacttccg tgacattccg tcgattctat cacttgcgtt
    22741 cgaacgccga ggcgtacatg ctctcgacgt agtccttcac catgcgccgc gcggagaagc
    22801 gcggcatcac ggtccggatc gactccttga ccacgcgcag ccaggcgtgc ggcacgcccg
    22861 ctgcgtcgcg gtcgtagaag gtcgggacga tctgctcctc gaggatccgg tagagcgcat
    22921 cggcgtcggc cgcgtcggcc gcgccgtggt cgccctcggg cgcgccgccg tcgaccagcc
    22981 acccgttcga ccccgcgtaa ccctcggccc accacccgtc gccgatgctg cagtgcggga
    23041 cgccgttgat ggccgccttc atgccgctcg tgccgctggc ctcgagcggc ttgcgcggcg
    23101 tgttcatcca gacgtcgcag ccctgcacca ggaagtgcgc cacgtggagg tcgtagtcgt
    23161 ccacgaaggc cacccgcccg ccgaacccgt tgtccatcgc ccgcttgtag atccgctgca
    23221 ggtggtgctt gccgttgtcg tcggccgggt gcgacttgcc cgcgaacacg aactggagcg
    23281 gctgccggaa cttggagagg atgcggcgga ggcgctcggg gtcgtggaag agcagctcgg
    23341 gccgcttgta ggccgtgaac cgccgggcga agccgatggt gagggcgtcg gggttgagca
    23401 gggtgccggc cgccaccacc tgacccgggc tgaccgactc gttggtccag cggttgcgcg
    23461 cccgctcgcg gatgaacgcg aacaggtagc tcctgagctg ctgccgcacc tgccacagct
    23521 cggcgtccgg gatctcgtgc accttctccc agagggccgg gtcgtcgtgg cggctgcgcc
    23581 agtcgggcgc gatataggcc tcgaagagct tcgacagctc cgccgcaacc caggtggcca
    23641 cgtggacgcc gttggtgatc gcccggaccg gctgctcgcg cttcagctcg ggccagagcg
    23701 gcgaccacat cgcctgcgtg acgtcgcgat gcagcacgct gaccgcgttg accgatcccg
    23761 ccgagcggat ggccagcgcc gtcatgttga actgcatccc gttgccgctg ttgttgtcgt
    23821 aggccccgag cgccaggaag ttgtcgcgca gcccgccgag actgccccac gcgccagcga
    23881 ggtaggtctc gacgaggtgg aacgggaagg cgtcgtggcc ggccggcacc ggcgtgtgcg
    23941 tggtgaacac cgtggtccgg cgcacctcct cgaccgcctg gtcgaactgg tagccgcgct
    24001 cgaggaactc gcggattcgc tggaggacga cgaaggccgc gtggccctcg ttgaggtgcc
    24061 agacggccgg ctcgacgccc atcgccctga gcgcccgcac gccgccgagg cccagcacga
    24121 tctcctgctg gatccgggtg tcgtggttcc ccccgtagag ccgcgccgac agctcgcggt
    24181 cccacggagc gttctcgggc aggttcgtgt cgagcaggta gagcttcacc cggccgccgc
    24241 gcaccaccca gaccgcggcg agcacgcttc ggtcgccgat cgggacggcg accacgcacg
    24301 gggttccgtc gggccggatg gcgggctcga tcggcgcgtc cagccagtcg agcgtctcgt
    24361 aggtctcctg ctgccacccc tcggcggtca ggtgctggtg gaagtagccc tgggggtaca
    24421 tgaagccgac acccaccagc gggatcccga ggtcgctcgc ctccttgcag gtgtcgccgg
    24481 ccaggacgcc caggccgccg gcatacaggg gaagcgactg gtgcagggcg aactcggccg
    24541 agaagtacgc cacctggccg ccgttggcct gcccgccgaa gcgcagcccc caccaggtgt
    24601 cgcgtgcaga ccgtgccgcg tcgagctcgg acatcaccga gtcgtacagc gacaggaacg
    24661 ccgggtcctc cgccgcttcc gccagccgct cgcgcgggat cagccgcagc atccggaccg
    24721 ggttgtgggc ggtcagccgc cagagcgggt agtcgagatc ccggaacaca tcacgcgcgc
    24781 ggtagtgcca gctccaccac aggtcggtgg ccagctcttc gagacgggcg atgcgcggag
    24841 ggaggggagg gaggccggga tatcgttcca tgttcctcgt cttggattat cgcatcaggg
    24901 gcggctcgag ccgccgaagc tcaatgttac actggcccgc gcgcgcccca cctcatccgt
    24961 cgccccgcgg gcgcggagcg gcagccggtc ttttcccccg gggacaggag cagccatgtc
    25021 gtcagaacaa ctcttccgtg acgtgtgggc ggcactcgtc ctgtcggcct tgccgtggat
    25081 ggcggcggcg gcacgggcgc aggaacctcc gagccgggag ctggagcacc tcaactggat
    25141 ggaggttcgc gagctggtcc cggcgaggat acgaaccgtc ctcctgccgc tcggtacgct
    25201 cgagggccac ggcgtggcgg tgaacggcag cgacacgatt gcgccgctcg cgatggcccg
    25261 cgccatcgcg ccgaaggtca acgcgctcat cgccccggcc gtcccctacg gcatcaccgg
    25321 ctcgctcgag gcctacccgg gcgggctgac cattcccgac gaggcatacc ggccgtacgt
    25381 tcgcgccatc cttcacggtc tcgcgcgcag ccgcttcaag aacgtcatcc tgctcaacgg
    25441 ccacggcggc aacaccgctg ccctgaacgc cgtggcggcc gaagtcgggc gcgaggcggg
    25501 catccgcacg ctggtcgtca actggtggac gttctgctcg gacgtgacgc tcgaggtgtt
    25561 cggggaggac ggcggccacg ccggcgagaa cgagaacgcc tacgtgatgg cggtggatcc
    25621 ctcgaacgtc cgccgcgagc gctatacagg gccggagttg gccatcgcga accccgcgcc
    25681 gggcgcctgg agcgcgtacc cgagcccggc cagcatcctc ttgtacaagc ccggtgaggg
    25741 cctgccgcgc ttcgacgcga tcaaggcgaa ggagtacttt gacaaggtga atgcgaaggt
    25801 cgcagcgctg gtcgaagaca tcgtcgggcg ctgggacagg gcggggatct agaaggctca
    25861 aggctcacgg ctcaaggcac ggggctcacg gctcactgct cagggtgcgg ggttcggggc
    25921 ttcggactgc ggaggagagg cgcgcatgag cggcaagatg aagcggaggg agttcgttcg
    25981 ggccggggcc gcggcggtgg cggcggcagc gccccgcgcg gtgttcggcc aggcccctgc
    26041 ggtgtcaact cgcgcggtgc ggccggtcgt catctcgtcg gcgaacggga atcgcgcccg
    26101 caacggcggg cagcagacct gcgtcgagcg tgccttcacg atgatggccg gcgggtccga
    26161 cgtgctcgac gccctcatcg cgggcgtcaa cattgtcgag ctcgacccgg ccgacacgag
    26221 cgtgggctac ggggggctgc cgaacgcgga cggcgtcgtg cagctcgact cctgctgcat
    26281 gcacgggccg cgcaagcgcg ccggcggcgt cgccgcgatc gagggggtga agacgccctc
    26341 gctcgtcgcg cgcgccgtga tggacgcgac cgaccatcac ctgatcgtcg gggaaggggc
    26401 ccgggagttc gcgcgcaagc tggggttcga gatcctcgac gatctgaaca ccgagacatc
    26461 gcgcaggatg tggctggaat ggaagcggcg gaccgacccc ttgcactacc tcgatccgtc
    26521 ggaccgcgag atggcgatca agaaggtgag cctctcgatc atgcgcgagt tcggcgccga
    26581 ccccgagcac tactacggga ccatcaactg caacggcgtc aacgcgcgcg gcgaggtgtc
    26641 cggcgtgacg accaccagcg ggatggcgtg gaagatcccg gggcgcacgg gcgattcgcc
    26701 gatcctcggg gccggcctct acgtcgacaa cgaggtgggc gcggcggggt ctaccgggcg
    26761 gggagaggcg aacctgttca acctgtcgtc ctacctcgtc gtcgagaacc tgcggcgggg
    26821 catgcacccg aaggatgccg ggatggacgc gctgcggaga gtctcggcca acaccgtgga
    26881 aaagcggctg ctcacgcccg acggcctgcc gaacttccag ctgaatttct acgtgctcaa
    26941 cgcgcgcggc gagtacgcca gcgtctcgat gtaccagtcc acgtacgccg tctgcaccga
    27001 gtcggggccg cagacgctgg cgagcgaggc gctgatcccg agaacgatcc cggagtgatc
    27061 cgcgggcacg gaggcccgcg cagctgtcgg ccgggagagc ccatgaagcg aacgatcatt
    27121 tgcctggcgc tgctcgctcc ggcgatcgcg gccctggcgc aggaaggggc gtcgcggccg
    27181 cgcgcccgcg acctgggcgt cgggcctggc gtgttcgcgc cggggccgct caacgcgatc
    27241 accgacgtcg caggtgtcgc ggtcggccac gtcaccatcg ttcggggaga cgaggtgcgg
    27301 acgggggtga ccgcgatcct cccgcacggg ggcaacctgt tccaggacaa ggtggctggc
    27361 gcggtcttcg taggcaacgc cttcggcaag ctcgccgggt ccacgcaggt cgaggaactg
    27421 gggacgatcg agacgccgat cgtcctcacc aacacactct cggtcggcgt cgccatcgac
    27481 gccgtggtcg gctacacgct ggaccaggac ggcaacggcc gggtgcagtc ggtcaacgcg
    27541 ctggtcggcg agaccaacga cggaggactc aacgacatca ggggccgcca cgtcacacgc
    27601 gagcacgtgc tggaggcgat ccgggaggcg cggaccgggg aggtcgagga gggatcggtc
    27661 ggcgccggca cgggaacccg ctgctacggg tggaagggag gaatcggcac ctcctcgcgg
    27721 cagctccccg agcggtacgg cagctatcgg ctgggcgtgc tggtgcagac caacttcggc
    27781 ggggtgctga ccatcgcggg ggccccggtc ggccgggaac tcggccgcca tgccttcgcg
    27841 gctccgggcg gaggacccga ggcaggcgca gacgggtcgt gcatgatcgt ggtcgccacc
    27901 gatgcacccc tcgacgcgcg cgacctgcgg cggcttgcgg ctcgcgccgt cttcggcctc
    27961 gcccgaaccg gctcgtccta cagccacggc agcggcgact ttgccgtcgc cttcaccacc
    28021 tcgctcgaga tgcggacgaa ggcgggagag acagcgcccc gcgcgcggcg ggtgctgccg
    28081 tccgaagcgg tgtcgccgct gttccaggcc gcgctcgagg ccaccgagga ggccgtctac
    28141 aactcgctct tccgggcgac gaccgtgacg gggcgaggcg gccagcgggc cgacgcgctg
    28201 ccgatcgacc gcgtgcggga gatccttcgg cgctacaacc cgtcacctga ggccgtaccg
    28261 aagacgtgag aaatgtcacc cgcggggccg taccggaggc gtgaataacc tcacgcttcc
    28321 ggtacggccc cggggggcgt gagaaggcca cgcactcgcg atggcccctg tgtaacaggg
    28381 taattgtcgg gctggttcga ggacgcgatg cccacgtccg ccggccgcaa cccagctgat
    28441 caggccaaaa agtcccacct cagctttggc gcggcatttg ccttcgcccc tcgcatgaag
    28501 actcgattgt ctactcgcgt cgtgtttctc tcggtgtcgc tggcgatgtg cctggttgcg
    28561 gcgccggccc ttgcccagtc gggcggcggc ggcgtccggg gcggggtgag cggagatccc
    28621 gatcagttct acttcggggc gcacctcgac accggcccgc tggtcgagat gctcagcttc
    28681 cggcccaacg tcgagatcgg cctcggtgac gacctgacca cggtggctgc caacttcgag
    28741 ttcgtgtact ggattccgat ccggcgcacc gcgtggaaca tctacgccgg tggagggccc
    28801 gccctcaacg tctacaggtt cgatcgcggc cggaacgacg acacgaacac cgagcccggc
    28861 ttcaacgtcc tgattggcct ccaacactac cggggggtgt tcgccgagtt caagctcggg
    28921 ctcatcgaca gtcccgagat caagttcggc ctcggcttca gcttctgatc ctgacgcgtc
    28981 cacgctcgag gcagggaaga gagagaaggc agaacgcgac cgaggctgcc tcctcactct
    29041 tccttgtcct accctttcta cctcctacct tccttctcgc cttcactttc ctcctcgccc
    29101 ttcgtccggc cgcgctcggg aaccgcttcc tggttctctc gctcttcctg catgggccgg
    29161 ttggtgatac ccgccccggg ctgggccgag ccccccttgt cgctacgggg attctcgctc
    29221 cccggcacgg cgggcttctc cggctcgctc agctggcccg cgccgctctc catgtcggcc
    29281 gcagccggaa cgttcaggct ctcgcgcgag ccctccgcct tctcctgctc cgacctgtcc
    29341 tgctgcacca tcaacgcctc caaggtacga gaaatcgcca gttcgactgc cgtcgcaggt
    29401 agaaggcaag ggatgtaccg ggtagaatcg gatcacaatg atcagccaca actcgttcga
    29461 cacggaggcc gaactccagc tggccggggc accggtgttg gtgtacagcc ttgcgcggtt
    29521 ggagcgggca gggttcccgc gcgtcgggcg cctgccgtac tccctcaagg tcctgctcga
    29581 gaacctgctg cgccacgaga acgggcggtt cgccaccccc gaagacgtgc aggcgctcgc
    29641 ctcgtgggat ccggcacgga gagggaggcg cgagatcgcg ttcacgccgg cgcgcgtcct
    29701 gctgcaggac ttcaccgggg tgccggcggt cgtcgatctc gcggcgatgc gggacgggat
    29761 cgtccggttg ggcggggacc cggcgcgcgt gaaccccctg cagccggtgg agctggtcat
    29821 cgatcactcg gtgcaggtgg attaccacgc ccggaacgac gccttccatc tgaacgccga
    29881 gctcgagttc cgccgtaacc gggaacgcta cgtcttcctc cgctggggtc agaacgcctt
    29941 ctccaacttc cgcgtcgtgc cgcccgacac gggcatcgtt caccaggtga acctcgagta
    30001 cctcgcccgc gtggtgttcc gcgatgaggt gaacgggcgg gcgtcggcct tccccgacac
    30061 cctggtgggg accgattcgc acacgacgat ggtgaacggc ctgggcgtgc tgggatgggg
    30121 cgtcggcggc atcgaggcgg aggcgtgcat gctgggccag ccgatctcga tgctgatccc
    30181 cgacgtgctg ggcttccggc tggtggggcg cctggccgag ggagcgaccg ccaccgacct
    30241 ggtgctcacg atcaccgagc ggctgcgcaa gcacggcgtg gtgggcaagt tcgtcgagtt
    30301 ctacggggcg gggctcgact cgatgacgat cgccgaccgc gcgacgctga gcaacatgtg
    30361 tccggagttc ggcgccaccg cggcgatctt cccgatcgac gacatgacgc tcgattacct
    30421 gcggctcacg ggccgcgacc ccgggcaggt ggcgctcgtc gaggagtacg caaaggccca
    30481 ggggatgttc agggtcgccg gcgcgccgga agccgagtac agcgagacgc tgacgctcga
    30541 tctcgcggag gtggagccaa gcgtgtcggg accgcgccgg ccgcaggatc gcgtcggcct
    30601 gaagcacgcg aagcgatcgt tcgcagaggc gctcgaccgc gcgctggccg atcgccggcc
    30661 c
//
LOCUS       DAOPQX010000004        30502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_1950_length_30502_cov_4.710612, whole genome shotgun sequence.
ACCESSION   DAOPQX010000004 DAOPQX010000000
VERSION     DAOPQX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 30502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30502
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1950_length_30502_cov_4.710612"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="PKK95_00435"
     CDS             <1..1515
                     /locus_tag="PKK95_00435"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOC16699.1"
                     /translation="RPRRTLTREADDAARLRAGRLLAPPAGDRPLATALVVLGADALL
                     PRGRQGLTAWIALGGLGATAAALVPLVGAQAAAAAGLMAVDAFAGFFKLLFLLAAALT
                     ILMSTRYLSVEGARPGTYYFLVLCATLGMLFMASALDLVTQIIGLETMAVSFYILAGF
                     LKPNRRSNEAAVKYFLLGAFSLGLLLYGMSLLYGLTGTTMLRDIGPALAGLEGNRWLG
                     LAVILVVAGLGFKIAAVPFHMWAPDVYEGAPTPITAFLSVGSKAASFAMLLRLFFEAL
                     PAANADWALLFYALAILTMTVGNLAALTQSNLKRLLAYSSIAHAGYVLIGVVAATSVP
                     QVGLKATLIYLLVYAFMQIGAFGVVTMLRRHDVIGEELKDLSGLFTRSPLAGASMLLF
                     MLSLGGIPPTAGFMGKFWLFSAAIDGGYYWLAVIGVLNSAVSLYYYLRVVVFMWFKAE
                     AVGSPVVLGPSMGVLLAVTVVATLAIGVYPRALFDLADVSARALGAAGIAAMIR"
     gene            1612..1845
                     /locus_tag="PKK95_00440"
     CDS             1612..1845
                     /locus_tag="PKK95_00440"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOC16700.1"
                     /translation="MPGRKPGPPSLGSLGALSAVGIAFVLALLFGFLIGRALDGWLGT
                     SPLFLIVFFFLGLAAGVLNVIRTASAVTRNDRK"
     gene            1842..2270
                     /locus_tag="PKK95_00445"
     CDS             1842..2270
                     /locus_tag="PKK95_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOC16701.1"
                     /translation="MTRVRPGGDPLRSFERDSAVACLAMAGAGWALSGWRLDVAGGVL
                     AGGGLMAVSYAFIKGGVEGAARALAEAAGTSRRRVRLALAVVKFLTRYALLAVAAYGI
                     LTCFHLHPAGVLAGALSPFLGALGQLVRMSRARTRAGRPL"
     gene            2270..3079
                     /gene="atpB"
                     /locus_tag="PKK95_00450"
     CDS             2270..3079
                     /gene="atpB"
                     /locus_tag="PKK95_00450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOC16702.1"
                     /translation="MEKIHHTLWIVQFVNGLLGPLVASMLRPFGIVFEPGAEVIPDYV
                     VMALLILAALAVLALLIRSRLNVESPGRFQIVLEDALSFVHGMLDEYVGPKGRRYLPL
                     VGTMFVFILIGNLMGLVPGLMAPTSNINVTLGCALTVFVYYQFQGFKEQGVVGYIKHF
                     LAPPGAPMWIAPIYFPIEIISHLSRVLSLSVRLFGNIFGEELVILILASLVPFIVPLP
                     MMFLGIITSSLQAYIFTMLTMIYLAGAVVVDHGHDGHDAGVEHATADMAHA"
     gene            3194..3532
                     /locus_tag="PKK95_00455"
     CDS             3194..3532
                     /locus_tag="PKK95_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16703.1"
                     /translation="MRKSLVPTLTLLFVAMGLVAPVFAQEAGATGGGEIAKWSIITAG
                     FALAIAAAFGALGQSKGLSAAAEAIARNPSANADIRFALILGLVLIESLVIYVLLIAL
                     ILFFVNPFVG"
     gene            3525..4550
                     /locus_tag="PKK95_00460"
     CDS             3525..4550
                     /locus_tag="PKK95_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOC16704.1"
                     /translation="MADPWTAGGARRPRPFLSARRFPLAPIDALVVLATLVWGANYSI
                     VKVALRQVPELSFNALRLGIASVLFLGVLAFFPAARRRGQAAGVGGEPPRHWFPTARL
                     FTAREWLALIGLAVSGHFLYQLCFLGSVARTSVANSSLIVGTSPVAVSLLAAAVGQER
                     VSRLHWAGAAVSLSGVYLLVGTGAAVSRASAVGDLLMIAGVWCWAVYAVGSAPMLRRH
                     APIALTGYTMAIGSLLYLPAALPELARLQWAAVTPASWIALVYSAVFALFAAYIVWHT
                     AIQRIGNVRTAMYSNLIPLSALATSIVFLGDRLSPRQMLGAAAVLTGVALTRLRPVWR
                     ASLPAEE"
     gene            4667..5734
                     /locus_tag="PKK95_00465"
     CDS             4667..5734
                     /locus_tag="PKK95_00465"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOC16705.1"
                     /translation="MASTETRQEAQARARQIDAFRRELGELDREGVLRLDAGERERVE
                     RHHAALLAELADRFDVDRGESESQLSLGMRIAALLGALALSAGLVLFFYRVWGLLGTP
                     SQVAILAAVPLLALVAVEAAARRERTLYVASVLALLAGAAFVLDLSALGAIFNMAPSP
                     WVLLPWAAFALALAYTYGLRLMLAAGLLLALGLANALVAHAAGIAWAASAARPEPFLV
                     LGAAVFAGSFTALAGRREGFAETWRLVGALSLLLPILFLSVWTTVFSYHLLPETVLRL
                     LYDSGGFVLPVAAIWTGIRRGWPWVVRLSTAFLVVFTYAKCFDWWWELLPRYLFFLVL
                     GALAVGAIVLMGRLRRRVKAV"
     gene            5736..6542
                     /locus_tag="PKK95_00470"
     CDS             5736..6542
                     /locus_tag="PKK95_00470"
                     /inference="COORDINATES: protein motif:HMM:NF027432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4824 family protein"
                     /protein_id="HOC16706.1"
                     /translation="MTRGWWIAVGIVAAANAAAVGGAAWNRQGEPEAVLELTERELEL
                     PPAAAENTALALRLRWTDPGNERLVPVEQPSPPVAAAPPPAGGASSPIPQAPPVPAVP
                     AASWFDRAKLEEVGFDCSLPLDRANAARYRAMPPRRTYAVLEYEGEAWRSLERSRGSG
                     GRQSDRASRLVVVDAGPDRKALRARWPDRRRAVVVPATAALVVEGGQDGRYVLHGRVI
                     EIFPSQLNVPRPLNRVLAPLQRRTGDEGPARYRVRVIWGRRDPWIADVQP"
     gene            complement(6593..7531)
                     /locus_tag="PKK95_00475"
     CDS             complement(6593..7531)
                     /locus_tag="PKK95_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16707.1"
                     /translation="MLRSVAAVPAEIVGVFREPAAFAQAASGQYFVFDRGNHAVYGID
                     RALTSAWKILQIGPEIGRILQPTAFSLARNGTFVVADRPGPAERIQVFAPGGSLLGGF
                     TLPGRAEETITLEGVVLNGVGTLHYDGRRLLLSQPETGALVAEYSPSGGITRTFGDLR
                     KTGQEAERDVHLGLNSGMPLPAPGGFYYVFLAGVPAWRRYDETGRLVFERHIEGRELD
                     AIVAALPTEWRRRRTKDERLIPVIPPVVRTAAVDGRGRLWVSLVGAPVTYVYDADGEK
                     ARVVRFEAAGALSPASLFFSHTGRLLAAPGCYEFAP"
     gene            complement(8024..10903)
                     /locus_tag="PKK95_00480"
     CDS             complement(8024..10903)
                     /locus_tag="PKK95_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC16708.1"
                     /translation="MRSRTLALIVLLVASAVPLAAQGIQTGILAGTARDAGNLVLPGV
                     TVTVSSPALQGTRTAVTDGNGRYVIRGLPPGTYAVVFELPSMQTVTHTVVVELGRTAE
                     ANAVLNLAGITESVTVTGETTPAALVTPSGGANFTYKDINVMPAARTLSGIAELSPGL
                     TNNTPNAGQVTISGAFAYDNVFLIDGVDVNDNLFGTANNLFIEDAIEETQVLTSGISA
                     EFGRFSGGVVNAVTKSGGNVFSGSGRVNLTNDAWTRLTPYEQSRNTKKADKLNRTYEL
                     TLGGPILIDRLWFFGATRLASVLSTTTMQLSGKKFDTTDKNRRWEAKLTGRLASNHTL
                     QGSFTNNPREVSLRRGLGQVIDEHALESPDFPNNGIVVGYNGVLRSNLFGEVRWSRKH
                     FGFRNTGGSSTNIVDSPIWSYFGPFGTVTYNAPYWDQTDPEDRNNQQVAGSLSWFKGT
                     QRLGSHDIKVGFENFRSTRTGGNSQTATGWVFLADYKAAADGTPIYDSQGYMIPMFAE
                     GATEAEQWLPVRGAKIDITTNSFFIQDRWAVNRRLSFNAGVRYERVRGEATGNIVTVD
                     TDTIVPRLAGTVDIAGDGKYIFQATYAHYAGKYSEAQFAQNTNVGSPDYLGLYYIGPT
                     GEGRDFAPGFDPANYVPYAGSFPTANVFMDSGLSSPVTREFSTSIGAQLGRGYAKIIY
                     TNRSVGNFVEDFIELNNGTTTIERAGLTFGPFTNQIYRNTDAPQRDYQALLLEGRYPI
                     TSRWSLNGHWTLQLKNEGDYEGEGANTPGSTTIYGNYPEIRSPSRNYPIGRFDDYQRH
                     KVRLWTIYSLGLGRLGDIDTSLMFRHDSPTYYSLTASSVPLSAIQKARGAAYPDLPTS
                     QTLYFGMRGSQSFKSTNLFDLALHYQIPIWRTVRPWLKMELYNVANSLPLYRWNTAVS
                     VDNASAKDEMGLPTGYIKGSNYGKGTAVTHYPGARRFQMAFGFRF"
     gene            complement(11224..13461)
                     /locus_tag="PKK95_00485"
     CDS             complement(11224..13461)
                     /locus_tag="PKK95_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HOC16709.1"
                     /translation="MAGFRAQFEELASPGKAFASPGRRRSVPGQGSPMDRSMLDSGAA
                     EAGDRSAFQGLDRRRLVEAYRWMVLSRKLDDKEIQLKNQSRIFFQISGAGHEAVLVAA
                     SYSLRPGYDWFYPYYRDRALCLALGVTPYEMLLQAVGAADDPASGGRQMPSHWGHRRW
                     NIVSQGSPTGTQCLQAVGCAEAGRLYRRLESIPGREALHHPDEVVYVSLGEGATAEGE
                     FWESLNTACGRRLPVVYLVQDNGYAISVPVEVQTPGGDLSKIVRSFPGLHVAKVDGTR
                     FLESHAAMRDAVAYAREGRGPALVHATVIRPYSHSLSDDEKLYKTPAEREAEARRDPL
                     AVHARFLVGEGLASEEELQRITDEAERDVRDAAARALEAAKPAPDTATLWVFSPDVDP
                     ASAAFDTPEAPVGKPDTMVAAINRTLRDEMAANPRIVVFGEDVADATREEALSAVAGK
                     GGVFKVTHGLQRLYGATRVFNSPLAEANIIGRAVGMATRGIKPVVEIQFFDYIWPAMM
                     QLRDEMAMLRYRSGNNFSCPMVVRAPIGGYLKGGAPYHSQSGVSIFAHCPGIRIAFPS
                     SAVDAAGLLRTAIRCDDPVLFLEHKHLYRQTYNKGTYPGPDFMIPFGQGALRREGDDV
                     LVLTWGALVQRSLLAAQQAEKEGLSVAVFDLRSLIPYDWPGIAALVKRINRVVVVHED
                     QLTCGFGAELAARIAGELFEHLDAPVARVGSLDTPVAYCPDLEEVILPQTADVLKAVL
                     VTAAY"
     gene            13519..13869
                     /locus_tag="PKK95_00490"
     CDS             13519..13869
                     /locus_tag="PKK95_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOC16710.1"
                     /translation="MAPSAARLHRWDEIALEKITEMISRKIVTGDRLLMAHVYLKRGA
                     LVPIHRHDSEQVTYVLEGALRVHVDGEEMTVREGEVLRIPGGTPHQAEALDDTLELDV
                     FSPPRKDWLAPDDQ"
     gene            complement(13863..14603)
                     /locus_tag="PKK95_00495"
     CDS             complement(13863..14603)
                     /locus_tag="PKK95_00495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC16711.1"
                     /translation="MRRYPSSPYRSVSYSFGPGPVTPVIKLLIWTNVAFFVLAWVLPA
                     VRLYLGLMPEAVFGRYFLWQPVTYLFLHAGFFHIIFNMLALWMFGVELERLWGARFFL
                     KYYFVTGVGAGLTTLAFALVPGELGTSVRYSITVGASGAIYGLLLAYALYFPNRPIYL
                     YLLFPIPAKYFVMIMGAIALLSSMAANQGGVANLAHLGGLVCGYLYLKVWRTRPLAEV
                     KYRYFKWKMNHMRRRFDVYSGGRGPRIH"
     gene            complement(14738..16411)
                     /gene="murJ"
                     /locus_tag="PKK95_00500"
     CDS             complement(14738..16411)
                     /gene="murJ"
                     /locus_tag="PKK95_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOC16712.1"
                     /translation="MDASTSKTGPDGPDGPVVLPPAAEPAAGGGRRLARSAGIIGIAT
                     AGSRVLGLVRDQVLAYFFGAGNDMDAFNVAFRVPNLLRDLFAEGAMTAAFVPTFTRYL
                     RLHGREAAWRLGNLVVNTLAVVTVALVLLGIVFAEPLVTLFASDYRAVPGKFELAVSL
                     ARLTFPFLTLVALAAACMGMLNSLRRFFVPAFSPAMFNVGSIVTVLALVPLFASAGVR
                     PIYAVALGALVGGIAQILIQWPQLHREGYRYRPMLDARHPGLREVLVLMGPGTVGLAA
                     VQVNVLVNTILATGEGTGAVSWLNYAFRLMYMPIGLFGVSIATAALPAISTQAADNDV
                     AGMRGTVSSGLRMMLMLNVPATVGLVVLAEPIVALLFERGSFTATDTAATAAALTFYA
                     PGLIGYSAVKIAVPTFYALRDARTPVVVSALTVLINIVLNVTLVRVLGYRGLALGTAL
                     AAMFNAAVLLTLLRGRLAGIDGARLAVAVSKILAASVVMGTVAWAAAWGLQAFSGGTS
                     LTDRLLVAFGAIGAGMLALAAAARLLRVAEFDDALKLVRGRLLGGRASR"
     gene            complement(16549..17004)
                     /locus_tag="PKK95_00505"
     CDS             complement(16549..17004)
                     /locus_tag="PKK95_00505"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOC16713.1"
                     /translation="MSLMQDVSAAITAAMKQRDEQRLGALRLLKAALMNREVERGRAL
                     APDEELQVVTTLVKQRRESIEQFSRGGRQDLVDREQAELQVLQAYLPPALDDEALGRL
                     VQEVIVETGASSPKDMGRVMKTVMARIPAGAADGKAVSERVKTELARKA"
     gene            complement(17013..18140)
                     /locus_tag="PKK95_00510"
     CDS             complement(17013..18140)
                     /locus_tag="PKK95_00510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC16714.1"
                     /translation="MTLTTRAMAEISRYQQTDRRGVDTLVRRVFGHDAANASQLRWRW
                     QHALNPATPADGPLILIAREGPTIVGQQSSMPVFLQAAGREWRAGWATDLMVAPERRR
                     QGIGEMLFRAFDRSVDAALGLGMGPASRPLMQKLKWPYLHAVPCLVKPLTRRALRQPR
                     WPMPLNRLVSAATLPAVKLVSRARPLAAQVEPMRHFDERFTELWERLAPRFDLAVRRD
                     AGYLNWKFIEPPHVRYSVVALKRGETIGGYATYRHLREPLGRVTLLVDFLTDPDDAEG
                     LATLLRWVDREARAADSDKIRVFATHAAYRKGLRRSGYFTVKSTLELGVRLDSAVPPD
                     FYRNTSRWHVTAGDADHDPAPVMARAASGSVRAVGPAPSGK"
     gene            18199..18981
                     /locus_tag="PKK95_00515"
     CDS             18199..18981
                     /locus_tag="PKK95_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOC16715.1"
                     /translation="MQSQPVSVVVPTYNERGRIERLVTEVFSSLAAAGLLGEMVVVDD
                     NSPDGTGALADELSARFPLKTVHRAGKLGLGGAVMEGFAVAEGELLAVLDGDLSHPPA
                     AIPDLVAALHGLGVDMAVGSRYIPGGRWRNWPRKRWLMSRVACLMARPLTPVRDAMSG
                     LFVLRRDAVEGVRIASAGFKIGLELQMRGRVRSVAEVPYTFTDRESGSSKMSTREGVN
                     YLAQLSILYRLRLAGGTRPPRYHRVTPGELETLRAQSARRRT"
     gene            complement(19007..19738)
                     /locus_tag="PKK95_00520"
     CDS             complement(19007..19738)
                     /locus_tag="PKK95_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16716.1"
                     /translation="MNQLYPALAAAVLAVGLAAPAHGGQVQIQIRDGRITLQARDASP
                     REILAEWARVGQTRIVNAERVPGGPLTITLTDVPEAKALDLVLRSAGGYLAAPRAAGA
                     TASSQYDRIVVLAIARPPAGVPAPAAEQSNPANRFQRPARMRPQPQQPAVLLDDQDEP
                     VPQEPEPMEEPQGASQPGMPTQPAGAGGNVPGTPASLTSPTPGMPTAAPSAARPGMPT
                     PPPRTPGQEQQPPQESRQPQEEPRQ"
     gene            complement(19804..20766)
                     /locus_tag="PKK95_00525"
     CDS             complement(19804..20766)
                     /locus_tag="PKK95_00525"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOC16717.1"
                     /translation="MRALITGGAGFVGSHLSELLLEQGHEVFVLDNLSTGSIDNIAHL
                     KSHPAFHYTIDSVDNEPLLAELVDQGDVVFHLAAAVGVKLIVEQPVHTIETNVRGTEV
                     VLKHANKKKKLVLIASTSEVYGKSVAVPFSEEADLVLGPTTKHRWAYACSKAIDEFLA
                     LAYWKEKKLPVVIARLFNTVGPRQTGQYGMVIPTFVRQALAGRPITVYGDGTQTRSFT
                     YVGDVVAALARLAVEPRAVGRVFNIGNGHEISIRELAERVKALAGSSSQIVTIPYEQA
                     YEEGFEDMPRRVPDITRLHELIGYEPRVQLDEILERVIEYFQKR"
     gene            complement(20794..21654)
                     /locus_tag="PKK95_00530"
     CDS             complement(20794..21654)
                     /locus_tag="PKK95_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC16718.1"
                     /translation="MASPTDTSVVIPAYNEAPVIAQVIEGLASTAAWREIIVVDDGSA
                     DGTAEAACAAGARVVRHPYNKGNGASVKTGIRQARGEFVLVIDADGQHPPSEAVRLVS
                     RLGEYDLVIGARAGTTQANPARRLGNAALNGVAAYMTGRSIPDLTSGFRAARREHLLE
                     FLHLLPNRFSTPTTTTLAFLKAGYNVAFEPIEARRRVGQSKIRLLRDGGKFFLIILKV
                     VTLFSPLRIFLPISLASFALGAGYAAWTVATQRHITNSSVLLIMLAVIVFLVGLVSEQ
                     IAALRFEGRR"
     gene            complement(21679..22725)
                     /locus_tag="PKK95_00535"
     CDS             complement(21679..22725)
                     /locus_tag="PKK95_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC16719.1"
                     /translation="MGVRTVLVCAVQVPLVEGGAESLSRELVRELRARGYRSELIALP
                     YKWYPKEEIFTHAAAWRLLDLSEVTGQRIDCVIATKFPSYCVRHPNKSIWLLHQHRAA
                     YDLCGNPLCADFELNELDIAARERLIELDTRMIREARRVYTIGAVPTARLERYNGLSA
                     PPLHHPPRLAARLRPGPYGDYVLSVGRLEALKRVDLAIRALAAGPARLRLVVAGEGPH
                     RPDLERLATELGVGDRVQFAGRVDDDTLIELYANALAVVFAPVDEDYGYITLEAMLSA
                     KPLITARDAGGPLEFVQDGVNGWVCEPEADSIAAAFARLDADRGLAASFGLAARERAA
                     VVTWDGVIEKLVGE"
     gene            complement(22764..23858)
                     /gene="ychF"
                     /locus_tag="PKK95_00540"
     CDS             complement(22764..23858)
                     /gene="ychF"
                     /locus_tag="PKK95_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOC16720.1"
                     /translation="MLRTALIGFPSAGKTTLFQLMTSHGEAVRPGHAKFEAAIGVAKV
                     PDERLDGLSELYNPRKHTPATVEFADLAGAGRTAAQALLDVAPFRNADALVHVVRAFR
                     DPTVAHHSDTLRPDADAQAMEDELILADLGVVEKRLERIDRDLKKGRSADLERERDLL
                     QRCGRALEDGAPLRGLELSVDERKLLRGFQFLSAKPLLVVLNLDESDVPALVDPAAAS
                     GRFNLSPFLARAATGLVAVCGRIELEIAQLDEADRTAFLRDLGLDEPGVQRVIRTTYD
                     LLGYISFLTAGEDECRAWSIPRGTTAQAAAGEIHTDIARGFIRAEVVRYEHLVARGSL
                     AACREHGEVRLEGKDYVVADGDVINFRFAT"
     gene            24018..24455
                     /locus_tag="PKK95_00545"
     CDS             24018..24455
                     /locus_tag="PKK95_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16721.1"
                     /translation="MLRGNCPAKIDEKGRLKIPQAFRQLIEREWGRELFVTSVNGDHV
                     RIYPMPAWERLEKQLGGGPWDPATKRFFKAVNYYGQTAELDNQGRVLIHPHLREKAGT
                     LGEVDVLGEVDHLAVWNHERLEADVAANPVTEADEFALAQKRA"
     gene            24470..25357
                     /gene="rsmH"
                     /locus_tag="PKK95_00550"
     CDS             24470..25357
                     /gene="rsmH"
                     /locus_tag="PKK95_00550"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOC16722.1"
                     /translation="MPTHVPVMPAEVLEALAPGRGGLFVDGTVGLGGHAAAILQAGAA
                     RLIGIDRDRDALDLARQRLAEWDERVTLLHGDYRRLDTLLDAEGVAAADGILIDLGLS
                     SFQLEAEGRGFSFQRDEPLDMRMDRTQGPTAAELLAALDERELADLIFGLGEERYSRR
                     IARAIVAAREEAPIATTGRLADIVRRAVPRKGYSRIDPATRTFQALRIRVNGELDGLD
                     AFIETACARLAGGGRLAVIAFHSLEDRVVKHTMRRLAGGEGSALRLLWRKARKPGEAE
                     VDANPRARSAKLRAIERVT"
     gene            25406..25807
                     /locus_tag="PKK95_00555"
     CDS             25406..25807
                     /locus_tag="PKK95_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOC16723.1"
                     /translation="MDGRIADVMGSGISPAPQNRAIVLEVDQARQRDFKRWLLVFAVL
                     VAAASFDACQRYGIVEHGFRLDQIQKLRDAEDAQARHLRLERESLREPSRIEEIATRE
                     LHLVVPALADTRVIERIVPPEQPPSSVVASR"
     gene            25817..27844
                     /locus_tag="PKK95_00560"
     CDS             25817..27844
                     /locus_tag="PKK95_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOC16724.1"
                     /translation="MAQRVEPNLWRGTIGRRTAVVIVAFVVWAVAIEARLVYLQVIDH
                     ERMSAEAEEQQSRNVTLLAPRGEILDRHGDLLAYSVDLDTIGAAPREAGDPQKAVARL
                     CAALGDCTRDEVSTLVKRFRSGRGYVYVRRKVTAEEARRVRDLNLPWVVFHKEPRRFY
                     PNLSLAAHVLGYVNLENNGLAGLESTYDQRVRGRDGMLLVMNDGQRRTFSSTVVRPPV
                     PGASLELTIDRYLQYIAERELRAGVAEHRARGGSVVIMDPHTGELLAIANSPSFNPNA
                     FAKSGPDQWRNRAVQDIYEPGSTFKMITASAALETGAFSPNDVIDTGSGVITLGSRRV
                     TEYEGHRYGALSLTDVMVKSSNVGAIKIGFGLGAERLGAFVKAFGFGTRLSPDFPGEN
                     AGMVWAPERWSQSSLMSVSMGYEIGVTPLQMAAAASTIANGGELIQPRVVRAVIEGGR
                     RVPVRPRVVRRVISPSTAASLVEIMEAVVERGTAKAARIDGYTVAGKTGTAAKLVNRY
                     YSRSDYNASFVGFLPSRQPALTILVVIDTPRGGKGYAGGVVAAPIFHRIAEAALRYLG
                     LPASVNPLPPLIVGGRPRPLEVAGPATPLLVAPVAGPLAEGQLRLPELRGLSGREAMR
                     VLARLGIGARINGDGLVVEQTPLPGTPVEPGTWCRLVLSRATAPTPPGAQP"
     gene            27841..29382
                     /locus_tag="PKK95_00565"
     CDS             27841..29382
                     /locus_tag="PKK95_00565"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOC16725.1"
                     /translation="MTLAELLAALGDLSSGVIADEPAMGRAVRALAYDSRRVSPGSVF
                     VAVRGQKADGAAFAAQALAKGALAVVAEAPPPPGVHATWVTVTDGRAAMARLAAAFYG
                     RPSREMQVVGITGTNGKTTTAYVLGALFEAAGTRCGMLGTVVYRTGTGERAATRTTPE
                     AIDIQQLLREMVAGGCGACVMEVSSHALALRRADEIEFAAAIFTNLTRDHLDFHRDME
                     SYFAAKRRLFELLGPGVPAAINLDDPRGATLASLVSRPVTYAIDRPADVVPEGLAQSL
                     RGVTFEARTPAGPVRIRSRLVGRPNVYNLLSAVTAAVSLGIPAPRIEEAIEALEGVPG
                     RFQVVSTEQDDITVVVDYAHTDDALKNLLETARPLASQRLITVFGCGGDRDRTKRPLM
                     GAVAARLSDFVIVTSDNPRSEDPQQIIDEIKRGIVVPPERWRPSAPNAPQKYAPPRAT
                     PHLAIVDRRLAIERAVEMAKPDDVVLLAGKGHEKYQVIGDRELPFDDVAVAREALARR
                     RGRRA"
     gene            29379..>30502
                     /gene="murF"
                     /locus_tag="PKK95_00570"
     CDS             29379..>30502
                     /gene="murF"
                     /locus_tag="PKK95_00570"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOC16726.1"
                     /translation="MSGQTGEAGRHALVVTGADVVSAMRGRRLHGNPNAGITGFTIDS
                     RRTRPGDLFIAIRGDRFDGHAFVASALAAGAGGAVVMEAPPGLTPEVLGDRLLIAVPD
                     TTLGLQALARSIRRRSGARVVAVTGSAGKTTTKEMIAAFLSLAGHVMRSPGNLNNHIG
                     LPLSLLELRHGFDAAVVELGMNHAGEIDRLVSIAEPDVRVWTNVAEVHTEFFPSIEAI
                     ADAKAEVLNGATTGTVLVANADDPRVVARMGSFPGRTVTFGFSPGATVRAEQVEDAGL
                     GGMRARVTTPSGTTAWSVALPGRNNLLNSLAAAAVGLEFGVPLAAMAEAASGLAPSAH
                     RGELVRLARGIVVLDDSYNSNPRALEAALDGVDVLVLSDYA"
BASE COUNT         4354 a        10567 c        10852 g         4729 t
ORIGIN      
        1 cggccgcgcc gcacgctgac tcgggaggcc gatgatgccg ccaggcttcg cgccggaaga
       61 cttctggctc ctcctgccgg agatcgtcct ctggcaaccg ccctggtggt gctcggcgcc
      121 gacgccctgc tgccgcgcgg tcgccagggg ctgaccgcct ggatcgcgct gggcgggctg
      181 ggggccaccg ccgcggcgct cgtgccgctg gtcggtgccc aggccgccgc cgccgccggc
      241 ctgatggcgg tggacgcgtt cgcgggcttc ttcaagctgc tgttcctgct ggcggcggcc
      301 ctcaccatcc tgatgtccac ccgctacctc tcggtggagg gggcgcggcc gggcacctac
      361 tacttcctgg tcttgtgcgc cacgctcggc atgctgttca tggcgagcgc cctcgacctg
      421 gtgacgcaga tcatcggcct cgagacgatg gccgtctcgt tctacatcct cgccggcttc
      481 ctgaagccga accgccggtc gaacgaggcc gcggtgaagt acttcctgct cggcgccttc
      541 tcgcttggcc tgctgctcta cgggatgtcg ctgctctacg gcctcaccgg gaccaccatg
      601 ctgcgggaca tcggccccgc gctggcgggc ctcgagggca accgctggct cggcctcgcg
      661 gtgatcctcg tggtggcggg gctcggcttc aagatcgccg cggtgccgtt ccacatgtgg
      721 gcgcccgacg tgtacgaagg ggcgccgacg ccgatcacgg cgttcctctc ggtcggctcc
      781 aaggccgcct ccttcgcgat gctcctgcgc ctgttcttcg aggcgctgcc ggcggcgaac
      841 gccgactggg ccctgctgtt ctacgcgctg gcaatcctca ccatgacggt cgggaacctg
      901 gcggcgctga cgcagagcaa cctcaagcgg ctgctcgcgt actcctcgat cgcacacgcc
      961 gggtacgtgc tgatcggggt cgtggcggcg accagcgtgc cgcaggtcgg gctcaaggcg
     1021 acgctcatct acctgctcgt ctacgcgttc atgcagatcg gcgccttcgg cgtggtgacc
     1081 atgctccggc ggcacgacgt gatcggcgag gaactgaagg acttgagcgg cctcttcacg
     1141 cggagcccgc tggccggcgc gtcgatgctg cttttcatgc tctcgctcgg cggcatcccc
     1201 ccgaccgcgg ggttcatggg caagttctgg ctcttcagcg ccgcgatcga cggcggctac
     1261 tactggctcg ccgtcatcgg ggtcctcaac agcgccgtct cgctttacta ctacttgcgc
     1321 gtggtcgtct tcatgtggtt caaggccgag gcggtcggct cgccggtggt gctcggcccc
     1381 tccatggggg tgctgctcgc ggtgaccgtc gtcgccacgc tcgcgatcgg cgtctacccg
     1441 cgcgccctgt tcgacctggc cgacgtctcg gcgcgggccc tcggcgcggc cggcatcgcg
     1501 gcgatgatcc gctgaaccgc agggctgaag cctgcggttg ccgctgatac ggggcagcgg
     1561 ggcagggccg agcccctttg tttgctgccg agagagggca gcgccgtgaa tatgccaggc
     1621 aggaagccgg gaccgccgtc gctcgggtcg ctcggcgccc tcagcgccgt gggcatcgcc
     1681 ttcgtgctcg cgctcctctt cgggttcctg atcgggcgcg ccctcgacgg atggctcggt
     1741 acctcgcccc tcttcctgat cgtcttcttc tttctcggcc ttgccgccgg cgtcctcaac
     1801 gtcatccgga cggcgagcgc cgtcacccgg aacgatcgca aatgacgcgg gtgcggcccg
     1861 ggggcgatcc cctccggagc ttcgagcgcg actcggcggt tgcctgcctt gccatggcgg
     1921 gggccggctg ggcgctgtcg ggctggcgcc tcgacgtggc cggcggggtg ctggcaggcg
     1981 gcggcctgat ggcggtcagc tacgccttca tcaagggggg cgtcgagggc gcggcgcgcg
     2041 cgctggccga agccgcgggc acttcgcggc ggcgtgtccg gctggccctg gcggtcgtga
     2101 agttcctcac aaggtacgct ttactggccg tcgcggcgta cggtatcctg acgtgttttc
     2161 acctgcaccc cgccggcgtg ctggccggcg cgctctcgcc gttcctggga gcgctggggc
     2221 agctggtgcg catgtcccgg gcgcgaaccc gggccggtcg tccgctctga tggagaaaat
     2281 ccaccacacc ctgtggatcg tgcagttcgt caacggcctg ttggggccgc tggtggcgtc
     2341 gatgctccgc ccgttcggca tcgtgttcga gccgggggcc gaggtcatcc ccgactacgt
     2401 cgtcatggcg ctgctgatcc tggccgccct cgcggtcctg gcgctgctca tccggtcgcg
     2461 cctgaacgtc gagagcccgg gccgcttcca gatcgtcctg gaggatgccc tgtcgtttgt
     2521 acacgggatg ctcgacgagt acgtcgggcc gaaggggcgg cgctacctgc cgctggtcgg
     2581 caccatgttc gtcttcatcc tgatcgggaa cctgatgggg ctggtgccgg gcctcatggc
     2641 ccccacgagc aacatcaacg tgaccctggg ctgcgcgctg accgtcttcg tgtactacca
     2701 gttccagggg ttcaaggagc agggggtcgt cggctacatc aagcacttcc tcgccccgcc
     2761 cggcgcaccg atgtggatcg cccccatcta ctttccgatc gagatcatca gccatctgtc
     2821 gcgggtgctg tcgctctcgg tccggctgtt cgggaacatc ttcggcgagg agctggtgat
     2881 cctgatcctg gcgagcctcg tgcccttcat cgtcccgctc ccgatgatgt tcctcgggat
     2941 catcacctcg tcgctgcagg cctacatctt cacgatgctg acgatgatct acctggccgg
     3001 cgcggtcgtg gtggaccacg ggcacgacgg acacgacgcc ggggtcgagc acgccaccgc
     3061 cgacatggcg cacgcctgag gggcgcggcg gggcggtgca cgggtggtcg aaggagcggg
     3121 gggcggcagg gcagaaggcc tgctcaaccc gacgcggata cggattcaaa acgacatcga
     3181 ggaggcgttc atcgtgcgca aatcgctggt gccgacactg acgttgctgt tcgtggccat
     3241 gggcctggtc gccccggtct tcgcccagga ggctggagcc accggcgggg gcgagatcgc
     3301 gaagtggtcg atcatcaccg ccgggttcgc gctcgccatc gccgcggcat tcggggccct
     3361 cggccagtcg aagggcctga gtgcggcggc cgaggcgatc gcccgcaacc ccagcgcgaa
     3421 cgccgacatc cggttcgcgc tgatcctggg gctcgtcctg atcgagtcgc tggtgatcta
     3481 cgtgctgctg atcgccctca tcctcttctt cgtcaacccg ttcgttggct gacccctgga
     3541 ctgcgggcgg ggcgcggcgg ccccgcccgt tcctctccgc ccgccggttt cccctcgcgc
     3601 ccatcgacgc gctcgtcgtc ctcgccacgc tcgtttgggg cgccaactac tcgatcgtca
     3661 aggtcgctct tcgccaggtt cccgagctga gcttcaacgc gctgcggctc ggcatcgcct
     3721 cggtgctgtt cctcggcgtg ctcgcgttct tccccgccgc gcgacggcgc gggcaggcgg
     3781 cgggtgttgg gggcgagccg ccgcggcact ggttcccgac cgcacgcctc ttcacggccc
     3841 gcgaatggct ggcgctcatc ggacttgcgg tcagcggcca cttcctctac cagctctgct
     3901 ttctcgggtc ggtggcccgg acgagcgtgg cgaacagctc gctgatcgtc ggcacctcgc
     3961 ccgtggccgt ctcgctgctc gccgccgcgg tcgggcagga gcgcgtctcg cgcctccact
     4021 gggccggcgc cgccgtgtcg ctctcgggcg tctacctgct ggtcggcacc ggcgccgccg
     4081 tctcgcgtgc gtcggccgtg ggcgacctgc tgatgattgc cggcgtgtgg tgctgggccg
     4141 tctacgcggt ggggtcggcg ccgatgctgc gccgccacgc gccgatcgcg ctgaccggct
     4201 acacgatggc catcgggagc ctgctctacc tccccgccgc gctccccgag ctggcccggc
     4261 tgcagtgggc tgccgtcacc ccggcgtcct ggatcgccct ggtgtactcg gccgtcttcg
     4321 ccctgttcgc tgcctacatc gtctggcaca ccgcgatcca gcgaatcggc aacgtgcgca
     4381 ccgcgatgta ctcgaacctc atcccgctca gcgccctggc cacctccatc gtgttcctcg
     4441 gcgaccgcct gtcaccgcgg cagatgctgg gggcagccgc cgtgctgacc ggggtggcgc
     4501 tcacccggct gcggccggtg tggcgcgcaa gccttcccgc cgaagagtag cgtccgtccg
     4561 tcacccgacg tatctccttt gcgcctctgc ggtgaacgtg atcatgaatg cggtgtgaga
     4621 cggttgtcgt ggacatcggg ctcgagagcc ggtgtatcct gacgccgtgg catctaccga
     4681 gacgcgccag gaggcgcagg cccgtgcgcg acagattgac gcgtttcgcc gcgaactcgg
     4741 cgagctcgat cgggagggcg tgctcaggct ggacgccggg gagcgtgaga gggtcgagcg
     4801 tcaccacgcc gcgctgctcg ccgagctggc agaccgcttc gacgtggatc gcggcgagag
     4861 cgagagccag ctgtcgctgg gaatgcgaat cgccgcgctg ctcggcgcgc tcgccctcag
     4921 cgccggcctc gtcctcttct tctatcgcgt atggggcctg ctcggcacac cttcccaggt
     4981 agcgatcctc gcggcggttc cgctgctcgc gctggtggcc gtggaggctg ccgcccgccg
     5041 tgagaggacg ctgtacgtgg catcggtcct cgcgcttctg gccggcgccg ccttcgtgct
     5101 cgacctttcg gccctcgggg cgatcttcaa catggcgccg tccccctggg ttctgctgcc
     5161 ctgggcggcc ttcgcgttgg cgctcgcgta cacctacggt ctgcggctga tgctcgccgc
     5221 ggggctgctg ctcgcccttg gcctggccaa cgcgctcgtc gcgcatgccg cggggatcgc
     5281 gtgggcggcg tcggcggcgc ggccggagcc gttcctggtg ctcggagccg cggtgttcgc
     5341 cgggtcgttc accgcgctgg ccggccgccg cgaggggttt gccgagacct ggcgcctcgt
     5401 tggtgcgctc tcgctcctgc tgccgatcct ctttctctcc gtctggacga cggtcttcag
     5461 ctatcacctg ctgcccgaga ccgtgcttcg gctcctctac gactccggcg gcttcgtgct
     5521 gccggtcgcg gcgatctgga cgggcatcag gcgcggctgg ccgtgggtcg tccggctctc
     5581 gaccgcgttc ctcgtcgtgt tcacctacgc gaagtgcttc gactggtggt gggaactgtt
     5641 gccccgctac ctgttcttcc tcgtgctcgg ggcgctggca gtcggcgcca ttgtcctgat
     5701 ggggcgcctg cggcgccgcg tgaaggcggt gtgagatgac gcggggctgg tggatcgcgg
     5761 tcgggatcgt ggcggccgcc aatgccgccg ccgtcggtgg agcggcctgg aaccggcagg
     5821 gagagcccga ggccgtgctc gagctgaccg aacgggagct cgagctgcct ccggccgcgg
     5881 ccgagaacac ggccctggcg ctcaggctgc ggtggaccga tccgggcaac gagcgccttg
     5941 tcccggtgga acagccgtcg ccgcctgtcg ccgccgcgcc tccgcctgcc ggcggggcgt
     6001 cctcgcccat cccgcaggcg ccccccgttc cggccgttcc cgccgcatcc tggttcgatc
     6061 gcgcgaagct cgaggaggtg ggcttcgact gcagcctgcc gctcgaccgg gcgaacgcag
     6121 cccgctaccg ggcgatgccg ccgaggcgga cgtacgcggt gctcgaatac gagggcgagg
     6181 cgtggcgttc tctcgagcgt tcgcggggat ccggcggccg gcagtccgac cgggcctccc
     6241 ggctcgtcgt ggtggatgca ggaccagacc ggaaggcgct gcgcgcgcgg tggccagacc
     6301 gccgaagagc ggtcgtcgtg ccggcaaccg ccgcgctggt cgtcgaaggc gggcaggatg
     6361 gccgatacgt cctgcacggc cgggtcatcg agatctttcc gtcccagctg aacgtgccgc
     6421 gccctctgaa ccgggtgctc gcaccacttc agcgccgcac gggcgacgag ggcccggcgc
     6481 gctacagggt gagggtgatc tggggacgga gggatccctg gatcgcggac gtgcagccgt
     6541 agctgcctcg ccgtccggcc cctcccaccc tccgcgctcc gcctcctgtc cgtcaggggg
     6601 cgaactcgta gcagcccggg gcggccagca agcgaccggt gtgcgagaag aacaggctgg
     6661 cgggcgagag cgcgccggcg gcctcgaagc ggacgacgcg cgccttctcg ccgtcggcgt
     6721 cgtagacgta ggtgaccggg gctcccacca acgaaaccca cagacgtcct cgcccgtcca
     6781 ccgccgccgt ccgcacgacc ggtgggatga ccgggatcag cctctcgtcc ttcgtgcggc
     6841 gccgccgcca ctcggtgggt agcgcggcga cgatcgcgtc gagctcgcgc ccctcgatgt
     6901 gacgctcgaa gacgagcctc cccgtttcgt cgtaccggcg ccacgccggc acgcccgcca
     6961 ggaagacgta gtagaagccc cccggggcgg ggagcggcat cccgctgttc aggccgaggt
     7021 gcacgtcccg ttcggcctcc tggcccgtct tcctgaggtc tccgaaggtc cgggtgatgc
     7081 cgcccgacgg cgagtactcc gcgaccaggg cgccggtttc gggctggctg aggagcaggc
     7141 gccgcccgtc gtagtgcagc gttccgacgc cgttcaggac gaccccttcc agcgtgatcg
     7201 tctcctcggc gcggcccggc agggtgaagc cgcccaagag cgagccgcct ggcgcaaaca
     7261 cctggatgcg ctcggcggga ccgggacggt cggccacgac gaatgttccg ttcctcgcga
     7321 ggctgaacgc ggtgggttgg aggatgcgcc cgatctcggg accgatctgc aggatcttcc
     7381 atgccgaggt cagggcccgg tcgatgccgt aaacggcgtg attcccgcgg tcgaacacga
     7441 agtactgccc cgaggccgcc tgcgcgaagg cagccggttc acggaagacg ccgacgatct
     7501 ccgccggcac ggcggcgacc gagcggagca cctgaacgcg gccgccggct gagggggatg
     7561 gcggggtcgc acgcgcctgc cgatgcttcc ccggtgcgaa cgacgtgagg gccggcgcgc
     7621 agcacgtgct gagaaaggat cgtcggttca cgacgccgac gctaccgccg gctcccgcca
     7681 cgcgtcaagc ttcaccgtag atcagatcgt gcgggaggtg cagacggtgc aggaggtgcc
     7741 gaaggcctgc cgcgccgtag ccgggcaccg cgcggcgaag gcgggtgcag tgctgatggt
     7801 gccggaagtg ccgaaagtgt tgcgctccct cgccagcagt catgtcgtca ctgcatttca
     7861 ccgcgcagag tgcgcagaga tctttggaag aatccgaaat ctcagcgtac gcctccgcgt
     7921 cctctgcgtt ctctgcgggt gaacgtacgt gggacaagcg gccgggccgg cctgcaggag
     7981 ccgaagccgc ctgcaggcct ccgcggccgc aatgctcccg gctctagaac cggaagccga
     8041 atgccatctg gaaccgccgc gctccggggt agtgagtgac cgcggttccc ttgccgtagt
     8101 tcgagccctt tatgtaaccg gtgggcagac ccatctcgtc cttggcgctg gcgttgtcaa
     8161 ccgacaccgc cgtgttccag cggtagagcg gcaggctgtt ggccacgttg tagagctcca
     8221 tcttcagcca gggccgcacc gtccgccaga tcgggatctg atagtggagc gccaggtcga
     8281 acaggttggt cgacttgaag ctctgcgacc cccgcatgcc gaagtacagc gtctgcgagg
     8341 tgggcaggtc ggggtacgcc gccccgcgcg ccttctggat agccgagagc ggcacgctcg
     8401 aggccgtcag gctgtagtag gtgggcgaat cgtgccggaa catcagggac gtgtcgatgt
     8461 cgcccaggcg tccgagaccc aggctgtaga ttgtccacag ccggaccttg tgccgctggt
     8521 agtcgtcgaa gcgcccgatg gggtagttgc gggaaggcga ccggatctcg gggtagttgc
     8581 cgtagatcgt cgtgctgccc ggggtgttgg ctccctcgcc ctcgtagtcg ccctcgttct
     8641 tcagctggag ggtccagtgg ccgttcaggc tccaccggga cgtgatcggg tagcggccct
     8701 cgagcagcag cgcctggtag tcgcgctgag gcgcgtcggt attgcggtag atctggttgg
     8761 tgaacggtcc gaaggtgagg ccggcacgct cgatcgtcgt ggtgccgttg ttcagctcga
     8821 tgaagtcttc gacgaagttc cccaccgagc ggttggtgta gatgatcttc gcgtagcccc
     8881 tgcccagctg cgccccgatc gaggtcgaga actcgcgggt gacgggcgag gacagccccg
     8941 agtccatgaa cacgttggcg gtcgggaaac tgcccgcgta gggcacgtag ttggccgggt
     9001 cgaagccggg ggcgaaatcg cggccttcgc cggtgggccc gatgtagtag aggcccaggt
     9061 agtcggggct gccgacgttg gtgttctgcg cgaactgcgc ctcgctgtac ttaccggcgt
     9121 agtgcgcgta ggttgcctgg aagatgtact tcccgtcacc cgcgatgtcc accgtgccgg
     9181 ccaggcgcgg gacgatcgtg tcggtgtcga cggtgacgat gttccccgtg gcctcgcccc
     9241 tcacccgctc gtagcggacg ccggcgttga acgacagccg gcggttgacc gcccagcggt
     9301 cctggatgaa gaacgagttg gtggtgatgt cgatcttcgc cccgcgcacg ggcagccact
     9361 gctcggcctc ggtcgcgcct tcggcgaaca tcgggatcat gtagccctgg ctgtcgtaga
     9421 tgggcgtccc gtcggcggcc gccttgtagt cggccaggaa gacccacccg gtcgcggtct
     9481 gcgagttgcc gccggtccgc gtgctgcgga agttctcgaa gccgaccttg atgtcgtggc
     9541 tgccgagccg ctgcgttccc ttgaaccacg acaggctgcc cgcgacctgc tggttgttcc
     9601 ggtcctcagg atcggtctgg tcccagtacg gcgcgttgta ggtgactgtg ccgaacggtc
     9661 cgaagtacga ccagatcggc gagtccacga tgttcgtgct gctgccgccc gtgttgcgga
     9721 agccgaagtg cttgcgcgac cagcgcactt caccgaacag gttcgagcgc agcacgccgt
     9781 tgtagcccac cacgatgccg ttgttgggga agtcggggct ttcgagcgcg tgctcgtcaa
     9841 tgacctgccc gaggccgcgc cgcagcgaca cctcgcgcgg gttgttcgtg aagctgccct
     9901 gcagggtgtg gttcgacgcg aggcgtccgg tgagcttcgc ctcccaacgg cggttcttgt
     9961 cggtggtgtc gaacttcttc ccgctcagct gcatcgtcgt ggtgctcagc accgacgcga
    10021 ggcgcgtcgc gccgaagaac cacaggcggt cgatgaggat cggcccgccc agggtgagct
    10081 cgtaggtgcg gttcagcttg tcggccttct tcgtgttgcg cgactgctcg tagggcgtca
    10141 gccgcgtcca cgcgtcgttg gtgaggttca cgcggccgct gccgctgaag acgttcccgc
    10201 cgctcttggt cacggcgttc accacgccgc ccgagaagcg cccgaactcc gccgagatcc
    10261 cggaggtgag gacctgcgtc tcctcgatcg cgtcctcgat gaacaggttg ttcgccgtgc
    10321 cgaacaggtt gtcgttgacg tcgacgccgt cgatcaagaa cacgttgtcg tacgcgaacg
    10381 cgccggagat cgtcacctgc ccggcgtttg gcgtgttgtt ggtcaggccc ggcgacagct
    10441 cggcgatccc cgagagcgtc cgcgccgccg gcatcacgtt gatgtccttg taggtgaagt
    10501 tggcgccgcc gctcggcgtg accagtgccg cgggcgtcgt ctcgcccgtg accgtcaccg
    10561 actcggtgat gccggcgaga ttgagtaccg cgttggcttc cgccgtccgg cccagctcca
    10621 cgaccaccgt gtgcgtgacg gtctgcatgc tgggcagctc gaacaccacc gcgtaggtgc
    10681 ccggcggcag cccgcgaatc acgtagcggc cgttgccgtc ggtcacggcc gtgcgcgtgc
    10741 cctgcagcgc cggcgacgag acagtcaccg taacccccgg cagcaccagg ttgccggcgt
    10801 cgcgtgcggt cccggccagg atgccggtct ggatgccctg cgcggcgagc ggcactgcgc
    10861 tcgccaccag cagcacgatg agagcgagag tcctcgagcg catacagtgc ttcctcccct
    10921 tgtttttatg aacaacgacg gcgagaagcg ggaagaacga aacggccgcg ccgcgcgaac
    10981 ccagagtccg cgtcagcgtg aatgccgccg ccatcctcgg cgagggccga gtgatgccgg
    11041 tatcgattga tcggcgaaga tccgaacctc aggtgtgcag agaggatgcc accatcgtcg
    11101 ccctccaagt cattgaaacc aaaggacgag ctgtcgacga ctcggctgcg gatccaaaac
    11161 aatggacggg aactacggcc gagtggatta taacccctcg cggaagccct ggcgagcgca
    11221 agatcagtac gctgccgtga cgagcacggc cttcagtacg tccgcagtct gggggaggat
    11281 gacctcctcg aggtcagggc aataggcgac cggcgtgtcc agcgacccca cgcgcgcgac
    11341 cggagcatcg aggtgctcga acagctcgcc ggcaatccgc gcggccagtt cggcgccgaa
    11401 gccgcacgtg agctgatctt cgtgaacgac gacgacgcgg ttgatgcgct tgacgagggc
    11461 ggcgatgcca ggccagtcgt acgggatcag gctgcggagg tcgaacaccg cgacgctgag
    11521 gccttccttc tcggcctgct gggcggcgag cagcgatcgc tggacgagcg ctccccatgt
    11581 cagcaccagc acgtcgtcgc cctcgcgccg cagggcgcct tgcccgaagg ggatcatgaa
    11641 gtcgggcccc gggtaggttc ccttgttgta ggtctggcgg tacaggtgct tgtgctcgag
    11701 gaacagcacc gggtcgtcgc agcgtatggc cgtgcgaagc aggcccgcgg cgtcgacggc
    11761 actgctgggg aaggcgatcc tgatgccggg gcagtgggcg aagatgctca cgcccgactg
    11821 gctgtggtac ggggcgccgc ccttcaggta gccgccgatc ggagcgcgaa ccaccatggg
    11881 gcacgagaag ttgttgcccg agcggtaccg cagcatcgcc atctcgtcgc gcagctgcat
    11941 catcgccggc cagatgtagt cgaagaactg gatctcgacc accggcttga tgccgcgcgt
    12001 ggccatcccc acggcgcggc cgatgatgtt cgcctcggcc agcggcgagt tgaacacgcg
    12061 ggttgcgccg taaaggcgct gcaggccgtg cgtcaccttg aacacgcctc ccttgcccgc
    12121 gaccgccgac agcgcctcct cccgcgtggc gtcggcgacg tcctcgccga acacgacgat
    12181 ccgcgggttg gcggccatct cgtcgcggag ggtgcggttg atggcggcaa ccatcgtgtc
    12241 gggcttgccc accggagcct cgggcgtgtc gaaggccgcc gaggccggat cgacgtcggg
    12301 cgagaacacc cagagcgtgg ccgtgtcggg tgcgggcttt gcggcctcga gcgcccgggc
    12361 ggcggcgtcc cgcacgtcgc gctcggcctc gtcggtgatg cgctgcagct cctcctcgga
    12421 cgccagaccc tcgccgacga ggaaacgggc gtgcacggcg agcggatcgc gccgcgcctc
    12481 ggcttcccgc tcggccggag tcttgtacag cttttcgtcg tcggagagcg agtgcgagta
    12541 cgggcggatc accgtcgcgt gcacgagggc cggcccccgc ccttcacggg cgtaggcgac
    12601 ggcgtcgcgc atggcggcgt ggctctcgag gaagcgcgtg ccgtccacct tggcgacgtg
    12661 cagcccgggg aaggagcgga cgatcttgga taggtcgccg cccggcgtct gcacttccac
    12721 aggcaccgag atcgcgtagc cgttgtcctg gaccaggtag acgaccggca ggcggcggcc
    12781 gcacgccgta ttgagcgact cccagaactc gccctcggcc gtcgccccct cgccgagcga
    12841 cacgtagacg acctcgtcgg ggtggtgaag cgcctcgcgg ccggggatcg actccagtcg
    12901 ccggtacagg cggccggcct cggcacaccc gaccgcctgc aggcactgcg tgccggtcgg
    12961 gctgccctgc gagacgatgt tccaccggcg gtgaccccag tgcgacggca tctggcgccc
    13021 gcccgacgcc gggtcgtccg cggcaccgac cgcctgcagc agcatctcgt agggtgtgac
    13081 gccgagcgcg agacagagcg cccggtcgcg gtagtagggg tagaaccagt cgtagcccgg
    13141 gcgaagcgag taactcgcgg caacgagcac cgcctcgtgc cccgcgccgc tgatctggaa
    13201 gaagatgcgg ctctggttct tgagctggat ctccttgtcg tcgagctttc gcgacaacac
    13261 catccagcgg tacgcctcga ccaggcgccg gcggtcgagg ccctggaagg cggagcggtc
    13321 gcccgcctcc gccgccccgc tgtccagcat cgatcggtcc atcggtgacc cctgccccgg
    13381 gacgctgcgc cgccggccag gcgacgcgaa cgccttcccc ggcgatgcca actcttcgaa
    13441 ctgggcgcgg aacccggcca ttatagcatc cgggcccggt gctataatcc cgctcaccac
    13501 ccgtcagcac cggagccgat ggccccttca gccgcccgcc ttcatcgctg ggacgagatt
    13561 gccctcgaga agatcaccga gatgatctcg cgcaagatcg tcacgggcga tcgactcctc
    13621 atggctcacg tgtacctcaa gcgcggcgcg ctggtgccca tccaccggca cgacagcgaa
    13681 caggtgacct acgtgctgga gggcgcgctg cgcgtccacg tggacgggga ggagatgacc
    13741 gtccgcgaag gggaggtcct gcgcatcccc ggcggcacgc cgcaccaggc cgaggcgctc
    13801 gacgacacgc tcgaactcga tgtcttcagc ccgccccgca aggactggct cgcccccgac
    13861 gatcagtgaa tgcgcgggcc gcgcccgccc gagtacacgt cgaaccgtcg ccgcatgtgg
    13921 ttcatcttcc acttgaagta gcggtacttg acctccgcca gcgggcgcgt gcgccagacc
    13981 ttcaggtaga gatacccgca caccaggccc ccgaggtggg ccaggttcgc cacgcccccc
    14041 tggttcgccg ccatcgacga cagcagcgcg atggccccca tgatcatgac gaagtacttc
    14101 gcggggattg gaaagaggag gtagagatag atgggacggt tggggaagta cagtgcgtag
    14161 gcgagcagaa gcccgtagat ggcgccggag gcgccgacgg taatcgagta gcggaccgag
    14221 gtgcccagct cgccgggcac cagggcgaag gcgagcgtgg tcaggccggc gccgacgccg
    14281 gtaacgaagt agtacttcag aaagaaccgg gcgccccaga ggcgttcgag ctcgaccccg
    14341 aacatccaaa gcgccagcat gttgaagatg atgtggaaga acccggcgtg caggaacagg
    14401 taggtgaccg gctgccagag gaagtaccgg ccgaagacgg cctcgggcat caggccgagg
    14461 tacagcctga cggccggcag cacccacgcg agcacgaaga acgcaacgtt cgtccagatg
    14521 agaagtttga tgaccggcgt gaccggcccg gggccgaagg agtaggaaac cgacctgtaa
    14581 ggcgacgaag gatagcgccg catgaacccc gccctgccgc ggccgcgcgg ccagctggcc
    14641 gaacctcgtt gcttccaacc ggattcccga tcgtaccacg ccgctcggcg aataggtccc
    14701 tcaggggagc aacttccatg ccccgcgcgg attttcctca gcgcgaagct ctgccgccca
    14761 gcagccggcc tcgcaccagc ttcagggcgt cgtcgaactc ggcgacccgc agaagccgtg
    14821 ccgctgccgc gagcgcgagc atccccgcgc cgatcgcgcc gaaggcgacc agcaggcgat
    14881 ccgtcagcga ggttccgccg gagaacgcct ggaggcccca cgcggcggcc cacgccaccg
    14941 tgcccatcac gacactcgct gcgaggatct tcgagacggc cacggcaagg cgggcgccgt
    15001 cgatcccggc gagccgtcct cgaagcagcg tcagcagcac cgcggcgttg aacatggcgg
    15061 ccagcgcggt gccgagcgcc agcccccggt accccaggac cctcacgagc gtgacgttca
    15121 gcacgatgtt gatcagcacc gtcagtgcgc tcacgacgac cggcgtccgg gcgtcacgca
    15181 gcgcgtagaa ggtcgggacc gcgatcttca ccgccgagta gccgatgagg ccgggcgcgt
    15241 agaacgtcag cgccgccgcc gtcgcggcgg tgtcggttgc ggtgaacgac ccgcgctcga
    15301 agagcagcgc gacgatcggc tcggccagca cgacgagccc gaccgtcgcg ggcacgttca
    15361 gcatcagcat catgcgcagg ccgctcgaca cggtccctcg catgcccgcc acgtcgttgt
    15421 cggcggcctg cgtcgagatg gcgggcagcg ccgcggtcgc gatcgagacg ccgaacagcc
    15481 cgatcggcat gtacatcagc cggaacgcgt agttgagcca cgacaccgcg ccggtcccct
    15541 cgccggtcgc aaggatggtg ttcaccagga cgttgacctg gaccgcggcc agtcccacgg
    15601 tgccgggccc catcagcacc agcacctcgc gcagcccggg gtgccgcgcg tcgagcatcg
    15661 gccggtatcg gtacccctcg cggtgcagct ggggccactg gatcaggatc tgcgcgatcc
    15721 cgccgacgag cgccccgagt gccaccgcgt agattggccg cacgccggca ctcgcaaaca
    15781 gcgggaccag cgcgagcacg gtgacgatcg agcccacgtt gaacatggcg ggcgagaacg
    15841 cggggacgaa gaaccgccgg agcgagttca gcatgcccat gcacgcggcc gccagcgcca
    15901 cgagcgtcag gaagggaaac gtcagccgcg ccagcgacac ggccaactcg aacttgcccg
    15961 gcactgcgcg gtaatcgctg gcgaagaggg tcaccagggg ctcggcgaag acaatcccca
    16021 gcaacaccag tgcgacggtc acgaccgcca gcgtgttgac caccaggttg cccagccgcc
    16081 aggccgcttc gcgcccgtgg aggcgaaggt aacgcgtaaa ggtcggcacg aaggccgccg
    16141 tcatcgcccc ctcggcaaac aggtcgcgca gcaggttcgg cacgcggaag gcgacgttga
    16201 acgcgtccat gtcgttgccc gccccgaaga agtaggcgag cacctggtca cgcacgagcc
    16261 cgagcacccg gctgccggcc gtggcgatgc cgatgatgcc ggccgagcgg gccagccttc
    16321 ggccacctcc ggccgccggc tcggcggcgg gcgggagaac gaccgggccg tccgggccgt
    16381 ccggccccgt cttactcgtg gacgcgtcca ttcagcctgt cagggtagca cggggggtac
    16441 aaaaggaagg gcctcttggc tcgagaggcc cttggtggag gatggacagg ctccgtggct
    16501 cttgctaccg acttagacgc cgcaatgccg ggaaaagttc tcctgccctc aggccttgcg
    16561 agccagctcg gtcttcaccc gctcgctgac cgccttcccg tcggccgcgc ctgccggaat
    16621 ccgggccatg acggtcttca tgaccctgcc catgtccttg ggtgaagagg cgccggtctc
    16681 gacgatcacc tcttgcacga gacggccgag cgcctcgtcg tccagagcgg gcggcaggta
    16741 cgcctgcagg acctgcagct cggcttgctc ccggtcgacc aggtcctggc gtccgccccg
    16801 ggagaactgc tcgatcgact cccgccgctg cttgacgagc gtcgtcacca cctgcagctc
    16861 ctcgtcgggc gcgagcgcgc ggccccgctc gacctcgcgg ttcatcaggg ccgccttgag
    16921 gaggcggagc gccccgaggc gctgctcgtc ccgctgcttc atggcggccg tgatggccgc
    16981 gctcacgtcc tgcatcagag acatgcccaa cctcacttcc cggaaggtgc ggggccgacg
    17041 gcgcgcaccg agcctgacgc cgcccgggcc atgaccggcg ccgggtcgtg gtcggcatcg
    17101 ccggcggtga cgtgccatcg cgaggtgtta cggtagaagt cggggggcac ggcggagtcg
    17161 agccgcacac caagctcgag cgtcgatttc acggtgaagt agcccgaccg gcgcagcccc
    17221 ttccggtaag cggcgtgggt ggcgaagacc ctgatcttgt cggagtcggc cgcgcgcgcc
    17281 tcgcggtcca cccaccgcag cagcgtcgcg aggccctcgg cgtcgtcggg gtcggtcagg
    17341 aagtcaacca gcagcgtcac ccgcccgagg ggctcgcgca ggtgccggta ggtcgcgtag
    17401 ccgccgatgg tttcgccccg cttgagagcg acgaccgagt agcgcacgtg cgggggctcg
    17461 atgaacttcc agttgaggta ccccgcgtcc cgccttacag ccaggtcgaa gcgcggggcg
    17521 agccgctccc agagttcggt gaaccgctcg tcgaagtggc gcatgggctc cacctgcgcg
    17581 gccaggggtc gcgccctcga cacgagcttg acggcgggca gcgttgccgc cgagaccagc
    17641 cggttgagcg gcatcggcca gcggggctgg cgcagcgccc gccgggtcag cggcttgacc
    17701 aggcacggca ccgcgtggag gtacggccac ttgagcttct gcatcagggg tcgggaagcg
    17761 gggcccatcc cgaggccgag cgccgcgtcg accgagcggt cgaaggcgcg gaagagcatc
    17821 tccccgatcc cctggcgccg gcgttcgggc gccaccatca ggtcggttgc ccatcccgcc
    17881 cgccattcgc gtccggctgc ctggaggaag accggcatgc tggactgctg gccgacgatg
    17941 gtcggcccct cgcgcgcgat caggatcaag gggccgtcgg caggggtcgc cgggttgagg
    18001 gcgtgctgcc accgccagcg caactggctg gcattggcgg catcgtggcc gaacacccgg
    18061 cgcaccaggg tgtcgacgcc gcgccggtcg gtctgctggt agcgggagat ctcggccatc
    18121 gctcgggtcg tcagcgtcat tgtaaatcgg tgtgcggcgg cgggtgcagc cgcccgtcgt
    18181 ttgtcgcaca atggcagagt gcagtcccag ccggtgtcgg tcgtcgtgcc gacctacaac
    18241 gagcgcggcc gcatcgagcg gctcgtcacc gaggtcttct cctcgttggc ggccgccggc
    18301 ctcctcggag agatggtggt cgtggacgac aactctcccg acggcactgg tgccctggcc
    18361 gacgagcttt cggcgcggtt ccccctgaag accgtgcacc gggccgggaa gctcggcctg
    18421 ggcggcgcgg tgatggaggg cttcgccgtg gcggagggcg agctgctggc ggtgctcgac
    18481 ggcgatctca gccatccgcc agcggcgatc cccgacctgg tggctgccct ccacggcctg
    18541 ggcgtcgaca tggcggtcgg cagccgctac atccccgggg gcaggtggcg caactggccc
    18601 cggaagcggt ggctgatgtc ccgcgtcgcc tgcctgatgg cgcggccgct gacgccggtc
    18661 cgcgacgcca tgtctggcct gttcgtgctg cggagggacg ccgtcgaggg ggtacggatc
    18721 gcctcggccg gcttcaagat cggcctcgag ttgcagatgc ggggacgtgt gcgctcggtg
    18781 gcggaggtcc cttacacctt caccgatcgc gagagcggca gcagcaagat gtccactcgc
    18841 gagggagtca actacctcgc gcaactctca atcctgtacc gtctccgcct cgcgggcggc
    18901 acacgtccgc cccgttacca ccgtgttacg cccggcgaac tcgagacgct gagggcgcaa
    18961 agcgcgaggc ggcggacgta gacggcgacg gctgcgtccg cgacctctac tgtcgcggct
    19021 cttcctgcgg ctgccgggac tcttgcggag gctgctgttc ctggccaggc gtcctgggtg
    19081 gcggcgtcgg catgcccgga cgggcggccg acggcgcggc ggtcggcata cccggggtcg
    19141 gcgatgtcag gctcgccggg gttccgggga cgttgccgcc ggcccctgcc ggctgcgtgg
    19201 gcatgcccgg ctgggaggcc ccctgcggtt cctccatcgg ctcgggttcc tgggggaccg
    19261 gctcgtcctg gtcgtcgagc aggaccgcgg gctgctgcgg ctgcgggcgc atccgggcag
    19321 gccgctggaa ccggttcgcc ggattggact gctcggcggc cggagccggc acgccggccg
    19381 gcgggcgcgc gatggcgagg acgacgatgc ggtcgtactg ggacgaggca gtggcgccgg
    19441 cggctctcgg ggccgcgagg taccccccgg ccgaccgcag gaccaggtcg agcgccttgg
    19501 cttcggggac gtccgtcagg gtgatcgtga gcggtccccc cgggacccgc tccgcgttca
    19561 cgatccgcgt ctgcccgaca cgcgcccact cggcgaggat ctcgcgcggc gatgcgtccc
    19621 tggcctgtag ggtgatgcgg ccgtcgcgga tttggatttg gacctgcccg ccgtgggcgg
    19681 gagcggcgag gccgacggcc agcacggccg ccgcgagcgc ggggtaaagc tgattcatga
    19741 caggcctcag gggtgactgg gccagtatag cccccgggag gaacccggcc aagctggagg
    19801 ggatcacctc ttctggaagt actcgatgac ccgctcgagg atctcgtcga gctgcacgcg
    19861 cggctcgtac ccgatcagct cgtgcaggcg cgtgatgtcg ggcacgcgcc ggggcatgtc
    19921 ctcgaagccc tcctcgtagg cctgttcgta ggggatcgtc acgatctggg acgagctgcc
    19981 ggcgagcgcc ttcacacgct cggccaactc ccggatcgag atctcgtggc cgttgccgat
    20041 gttgaacacg cggccgaccg cacgcggttc cacggccagc cgggcgagcg ccgcgaccac
    20101 gtcgccgacg tacgtgaagc tgcgcgtctg cgtgccgtcc ccgtagacgg tgatcggccg
    20161 ccccgccagc gcctgccgca cgaaggtcgg gatgaccatt ccgtactgcc cggtctgccg
    20221 ggggccgacg gtgttgaaca gcctggcgat gacgaccggc agcttcttct ccttccagta
    20281 cgcgagcgcg aggaactcgt cgatcgcctt gctgcaggcg taggcccagc ggtgcttggt
    20341 ggtcggaccg agcacgaggt cggcctcctc cgagaacggc accgcaacgc tcttgccgta
    20401 gacctccgag gtggaggcga tcaggacgag cttcttcttc ttgttcgcgt gcttgaggac
    20461 gacctccgtg ccccgcacgt tggtctcgat cgtgtgcacc ggctgctcga cgatcagctt
    20521 cacccccacc gccgccgcca ggtggaagac gacgtcgccc tggtccacca gctcggcgag
    20581 cagcggctcg ttgtcgaccg agtcgatcgt gtagtggaag gccgggtggg acttcaggtg
    20641 ggcgatgttg tcgatcgagc cggtcgagag gttgtcgagg acgaagacct cgtgaccctg
    20701 ctcgagcagc agctccgaga ggtgagagcc cacgaacccc gccccgcccg tgatcagcgc
    20761 gcgcatcgtg ccggactcct tccctgcgat ctcctacctg cggccctcga accggagggc
    20821 cgcgatctgc tcggacacca ggccgacgag aaatacgatc accgcgagca tgatcagcag
    20881 caccgacgag ttggtgatgt gccgctgcgt ggccacggtc cacgccgcgt atcccgcgcc
    20941 gagggcgaag gacgccaggc tgatcgggag gaagatccgc agcgggctga acagcgtgac
    21001 caccttcagg atgatcagga agaacttgcc accgtcgcgg agcagccgga tcttcgactg
    21061 gccgacgcgg cgccttgcct cgatcggctc gaaggcgacg ttgtagcccg ccttcaggaa
    21121 ggcaagcgtg gtggtcgtgg gggtggagaa ccggtttggc agcaggtgca ggaactccag
    21181 gaggtgttcg cgcctcgccg cacggaagcc cgacgtgagg tcgggaatcg accggccggt
    21241 catgtaggcc gcgaccccgt tcagcgccgc gtttcccagg cgccgggccg ggttcgcctg
    21301 cgtcgttccc gcccgcgcgc cgatcaccag gtcgtactcc cccagccgcg acacgagccg
    21361 gacggcctcg gacggggggt gctggccgtc ggcgtcgatg accaggacga actcgccgcg
    21421 cgcctgccgg atgccggtct tcaccgacgc gccgtttccc ttgttgtagg ggtggcgcac
    21481 gacccgcgcc cccgccgcgc acgccgcttc ggccgtcccg tcggccgacc cgtcgtccac
    21541 gacgatgatc tcccgccagg cggcggttga cgccagcccc tcgatcacct gggcgatgac
    21601 gggcgcttcg ttgtacgccg ggatgacgac ggaagtgtcg gtcggcgagg ccacgcgcgg
    21661 ctcctgtcgg ttcgggcgct actcgcccac cagcttttcg atcacgccgt cccaggtcac
    21721 gacggccgcg cgctcgcgcg ccgccaggcc gaaggatgcc gcgaggccgc ggtcggcgtc
    21781 gaggcgcgcg aaggccgccg cgatcgagtc ggcctccggc tcgcacaccc agccgttcac
    21841 gccgtcctgc acgaactcga gggggccgcc ggcgtcgcgc gccgtgatga gcggcttcgc
    21901 gctcagcatc gcctccagcg tgatgtaccc gtagtcctcg tccaccgggg cgaagaccac
    21961 cgcgagcgcg ttcgcgtaca gctcgatcag cgtgtcgtcg tccacgcggc cggcgaactg
    22021 cacgcggtcg cccacgccca gctcggtggc caggcgctcg aggtcggggc ggtgcggccc
    22081 ctcgccggcg acgaccagcc gcaggcgggc ggggccggca gccagcgcgc ggatcgcgag
    22141 gtccacgcgc ttgagcgcct cgaggcgccc gaccgatagc acgtagtcgc cgtacgggcc
    22201 gggccggagg cgggccgcca gccggggcgg gtggtgcagc gggggcgctg agaggccgtt
    22261 gtagcgctcg agacgggcgg ttgggaccgc gccgatggtg tatacgcggc gcgcctcgcg
    22321 aatcatccgc gtgtccagct cgatgagccg ctcgcgcgcc gcgatgtcca attcgttcag
    22381 ctcgaaatcg gcgcagaggg ggttgccgca gagatcgtac gcggcgcggt gctggtgcag
    22441 cagccagatc gacttgttcg ggtggcggac gcagtaggac gggaacttcg tcgcaatcac
    22501 acagtcgatc cgctggccgg tcacctcgct caagtcgagc aggcgccacg ccgcggcgtg
    22561 ggtgaagatc tcctccttgg gataccactt gtacggcagg gcgatcagct cgctgcggta
    22621 gccgcgcgcg cgcagctccc gcaccagctc gcggctgagg ctctcggcgc cgccctccac
    22681 gagcggcacc tgcacggcgc acacgagtac cgtccgaacg cccatcgatc gccagccccc
    22741 tcggcaggcc gcaccacagg ccgctaggtt gcgaaccgga agttgatcac gtcgccgtcg
    22801 gcgaccacgt agtccttccc ctcgagccgc acctcgccgt gctcgcggca ggcggcgagc
    22861 gagccgcgcg ccacgaggtg ctcgtaccgc accacctcgg cgcggatgaa accccgcgcg
    22921 atgtcggtgt ggatctcgcc cgccgcggcc tgcgccgtcg tcccgcgcgg gatcgaccag
    22981 gcccggcact cgtcctcgcc ggcggtcagg aacgagatgt agccgagcag gtcgtaggtg
    23041 gtgcggatca cccgctgcac gccgggctcg tccaggccca gatcgcggag gaacgcggtg
    23101 cggtcggcct cgtcgagctg cgcgatctcc agctcgatcc ggccgcacac ggccaccagc
    23161 ccggtcgccg cccgcgcgag gaacggcgac aggttgaagc ggccggaggc cgccgcggga
    23221 tcgacgagcg cgggcacgtc cgactcgtcg aggttgagca ccacgagcag cggcttcgcc
    23281 gagaggaact ggaagccgcg cagcagcttc cgctcgtcaa ccgacagttc caggccgcgc
    23341 agcggcgcgc cgtcctcgag cgcccggccg cagcgctgca gcaggtcgcg ctcgcgctcg
    23401 aggtctgccg aacgcccctt cttcaggtcg cgatcgatcc gctccaggcg cttctccact
    23461 accccgaggt cggcgaggat cagctcgtcc tccatcgcct gggcgtcggc gtccggcctg
    23521 agggtgtcgg agtgatgggc gaccgtgggg tcgcggaagg cgcgcacgac gtgcaccagg
    23581 gcgtccgcgt tgcgaaaggg ggccacgtcg agcagcgcct gcgcagccgt ccgcccggcc
    23641 ccggcgagat cggcgaactc gactgtcgcc ggggtgtgct ttcgcgggtt gtacagctcg
    23701 gacaaaccgt cgagccgctc gtcgggcacc ttggccacgc cgatggcggc ctcgaacttg
    23761 gcgtggcccg gccggacggc ctcgccgtgg ctggtcatca gctgaaagag agtggtcttc
    23821 cccgcggaag ggaagccaat cagggcggtc cggagcattc cggccgccat cgtatcagac
    23881 tcggccccga gggcgggggg cgaaagttta gtgaagttct tgcggctcaa aagtttccgc
    23941 cgattctcaa ttgacatccc gcaggccctg ccataccatc cgaagtggtc agaagtggag
    24001 cttagtggag cagagcgatg ctgcggggca actgcccggc caaaatcgac gaaaaggggc
    24061 ggctcaagat cccgcaggcc tttcggcagc tcatcgagcg ggagtggggg agagaactct
    24121 tcgtcaccag tgtaaacggt gatcacgtca ggatttaccc catgcccgcc tgggagaggc
    24181 tggaaaagca gctgggcggc gggccgtggg acccggccac caagcgcttc ttcaaggcgg
    24241 tcaactacta cggccagacc gccgaactcg acaatcaggg gcgcgtcctc atccaccccc
    24301 atctccggga gaaggccggc acgctgggcg aggtcgacgt gctgggcgag gtcgatcacc
    24361 tcgctgtctg gaaccacgag cggcttgagg ccgacgtggc ggcgaacccg gtgaccgagg
    24421 ccgacgagtt cgcgctcgcc cagaagcgag cgtgagcgtc ggggggcgta tgccgacgca
    24481 cgtccccgtg atgcccgccg aggtgctcga ggcgctcgcg cccggccgcg gcggcctctt
    24541 cgtggatggg accgtcggcc ttggcggcca cgccgccgcg atcctgcaag ccggcgcggc
    24601 caggctgatc ggcatcgacc gcgaccgcga cgctctcgac ctcgcgcgcc agcgcctggc
    24661 ggaatgggac gagcgcgtga cgttgctgca tggtgactac cggcggctcg acacgctgct
    24721 cgacgcggag ggggtggcgg ccgccgacgg gatcctgatc gacctcgggc tgtcgtcgtt
    24781 ccagctcgag gccgagggtc gcgggttcag tttccagcgc gacgagccgc tcgacatgcg
    24841 gatggaccgg acgcagggtc ccaccgcggc cgagcttctc gccgcgctcg acgagcgcga
    24901 gctggcggac ctgatcttcg gccttggcga ggagcgctac tcgaggcgca tcgcccgagc
    24961 gatcgtcgcg gcccgcgagg aggcgccgat cgcgacgacg ggccggctgg ccgacatcgt
    25021 gcggcgcgcg gttccccgca aggggtacag ccggatcgac ccggcgacgc ggaccttcca
    25081 ggccctgcgg atccgggtga acggcgagct ggatggtctg gacgccttca tcgagaccgc
    25141 ctgcgcgcgc ctcgccggtg gcggacggct cgcggtcatc gcgttccact cgctcgagga
    25201 ccgggtggtg aagcacacga tgcggcggct ggccggcggg gaaggctcgg cgctccggct
    25261 tctctggagg aaggcgcgga agccgggcga ggccgaggtg gacgcgaacc cgcgggcgcg
    25321 gagcgcgaag ctgagggcga tcgagcgggt gacctgagga aggcggcggc aggcatgtga
    25381 gccgcagggt ttctgggggg cagcgatgga cggacgaatc gcggacgtga tggggtcagg
    25441 gatcagcccg gcgcctcaga accgggcgat cgtcctcgag gtggaccagg cgcgtcagcg
    25501 cgacttcaag cggtggctgc tcgtcttcgc ggtgctggtg gccgcggcgt cgttcgatgc
    25561 ctgtcagcgc tacggcatcg tggagcacgg ctttcgcctg gaccagatcc agaagctgcg
    25621 ggacgccgag gatgcccagg cgcggcacct gcggctcgag agggaatcgc tgcgcgagcc
    25681 ctcgcggatc gaggagattg cgacgcgcga gctccacctg gtggttccag ccctcgccga
    25741 cacgcgggtg atcgagcgga tcgtcccgcc cgagcagccg ccatcgtcgg tcgtcgcctc
    25801 gcggtaggag cccgtcatgg cccagcgtgt cgaacccaac ctgtggcggg gcacgatcgg
    25861 gcgccggacc gccgtcgtga tcgtggcgtt cgtcgtgtgg gccgtcgcca tcgaggcgcg
    25921 gctcgtctac ctccaggtca tcgatcacga gcggatgtcg gccgaggccg aggagcagca
    25981 gtcgcgcaac gtcacgctgc tcgccccgcg gggcgagatc ctcgaccggc acggcgacct
    26041 cctcgcctac agcgtggacc tcgacaccat cggcgcggcc ccgagggagg ccggcgatcc
    26101 gcagaaggcg gtggcgaggc tgtgcgcggc gctcggcgac tgcacgcgcg acgaggtgag
    26161 cacgctcgtg aagcgcttcc gctcggggcg cggctacgtc tacgtccggc gcaaggtcac
    26221 cgcggaggaa gccaggcgcg tccgggacct caacctcccc tgggtcgtgt tccacaagga
    26281 gccccggagg ttctacccga atctctcgct cgcggcgcac gtcctcggct acgtcaacct
    26341 ggagaacaac ggcctggcgg gcctcgagtc gacctacgac cagcgggtcc gcggacgcga
    26401 cggcatgctg ctggtgatga acgacggcca gcggcgcacc ttcagcagca cggtcgtcag
    26461 gccgcccgtc ccgggcgcct cgctcgagct gaccatcgat cgctacctcc agtacatcgc
    26521 cgagcgcgag ctgcgcgccg gcgtggccga acaccgcgcg cgcgggggct ccgtcgtgat
    26581 catggacccc cacacggggg aactcctcgc aatcgccaac tcgccgagct tcaacccgaa
    26641 cgcgttcgcc aagtccgggc ccgaccagtg gcgcaaccgc gcggtccagg acatctacga
    26701 gcccggatcc accttcaaga tgatcacggc gtcggcggcc ctcgagacgg gcgcgttctc
    26761 gccgaacgac gtgatcgaca ccggcagcgg ggtcatcacg ctcgggtcgc ggcgcgtcac
    26821 cgagtacgag gggcaccggt acggggcgct gtcgctcacc gacgtgatgg tgaagtcgag
    26881 caacgtcggc gcgatcaaga ttggcttcgg gctcggcgcc gagcggctcg gcgccttcgt
    26941 caaggccttt ggcttcggca ccaggctctc ccccgacttc cccggcgaga acgccggcat
    27001 ggtctgggcg cccgagcggt ggagccagag ctcgctgatg tcggtgtcga tggggtacga
    27061 gatcggcgtg acgccgctcc agatggcggc cgcggcctcc acgatcgcca atggcggcga
    27121 gctgatccag ccgcgcgtcg tgcgagcggt gatcgagggc ggccgccgcg tcccggtccg
    27181 cccccgcgtc gtccggcggg tgatctcgcc atcgacggcc gcctcgctgg tcgagatcat
    27241 ggaggcggtc gtcgagcggg ggacggccaa ggccgcgcgc atcgatggct acaccgtagc
    27301 cgggaagacc ggcacggcgg cgaagctggt caatcggtac tactcgcgga gcgactacaa
    27361 cgcgtcgttc gtcgggttcc tgccgtcgcg ccagccggcc ctgacgatcc tggtggtcat
    27421 cgacacgccg cgcgggggga agggctacgc cggaggcgtg gtggccgcgc cgatcttcca
    27481 ccgcatcgcc gaggccgcgc tgcgctacct ggggctgccg gcgagcgtca acccgctccc
    27541 gccgctcatc gtcggcgggc gtccgcgtcc cctcgaggtg gcaggcccgg cgacgccgct
    27601 gctcgtcgcg ccggtggccg ggccgctcgc ggagggccag ctccggctcc ccgagctgcg
    27661 agggctcagt ggccgcgagg cgatgcgggt gctcgcccga ctggggatcg gcgcccggat
    27721 caacggcgac gggctggtgg tggaacagac cccccttccg ggcacgcccg tcgagccggg
    27781 cacctggtgc cgcctcgtgc tcagccgcgc aaccgctccc acgcctccag gggcgcagcc
    27841 atgacgctcg ccgagttgct ggccgcgctc ggcgacctct cgagcggcgt catcgccgac
    27901 gagccggcga tggggcgggc ggtgcgcgcg ctcgcgtacg actcgcggcg ggtgtctcca
    27961 ggcagcgtgt tcgtggcggt tcgcggccag aaggcggacg gcgctgcctt cgccgcgcag
    28021 gcgctggcga agggcgccct ggcggtggtg gccgaggcgc cgccgccgcc cggcgtgcac
    28081 gcgacgtggg tgacggtcac ggacggccgc gcggcgatgg cgcggctggc cgcggcgttc
    28141 tacggccggc ccagccgcga gatgcaggtg gtcggcatca ccggcacgaa cggcaagacg
    28201 acgaccgcct acgtgctcgg cgcgctgttc gaggcggcgg gcacccggtg cggcatgctc
    28261 ggcaccgtcg tgtaccggac cgggaccggc gagcgggccg cgacccggac cacgccggag
    28321 gccatcgaca tccagcaact gctgcgcgag atggtggcag ggggctgcgg cgcctgcgtg
    28381 atggaggtct cctcgcacgc gctcgccctg cgccgcgccg acgagatcga gttcgccgcc
    28441 gccatcttca ccaacctcac gcgcgaccac ctcgacttcc accgcgacat ggaatcgtac
    28501 ttcgcggcca agcggcggtt gttcgagctg ctggggcccg gcgttcccgc ggcgatcaac
    28561 ctggacgacc cgcgcggcgc cacgctggcc tcgctggtct cgaggccggt gacctacgcg
    28621 atcgaccgtc ccgccgacgt ggtgcccgag ggcctggccc agtcgctgcg aggcgtgacc
    28681 ttcgaggcac gcaccccggc ggggccggtg cggatccgct cgcgcctcgt cggccgcccc
    28741 aacgtgtaca acctcctctc ggccgtcacg gccgcggtct cgctcgggat cccggcccca
    28801 cgcatcgagg aggcgatcga ggcgctggag ggggtgccgg ggcgcttcca ggtcgtctcg
    28861 accgagcagg acgacatcac ggtcgtcgtg gattacgcgc acaccgacga cgcgctgaag
    28921 aacctgctcg agacggcgag gcccctggcg tcgcagcgcc tgatcaccgt gttcggctgc
    28981 ggcggcgatc gcgaccgcac caagcggccg ctgatgggcg cagtcgcggc acggctcagc
    29041 gacttcgtga tcgtgacctc cgacaacccg cgcagcgagg acccgcagca gatcatcgac
    29101 gagatcaagc gcgggatcgt ggtgccgccc gagaggtggc ggccgtcggc ccccaacgcg
    29161 ccgcagaagt atgcgccgcc tcgcgcgacg ccgcacctgg ccatcgtgga ccggcgcctc
    29221 gcgatcgaac gcgccgtcga aatggccaag ccggacgacg tggtcctgct ggcgggcaag
    29281 ggccacgaga agtaccaggt catcggcgac cgggagctgc cgttcgacga cgtggccgtc
    29341 gcgcgcgagg cgctcgcccg gcggcggggg aggcgggcgt gagcgggcaa accggcgagg
    29401 ccgggcggca cgcgctggtg gtgaccggcg cggacgtggt gtccgcgatg cgcgggcgtc
    29461 gcctccacgg gaacccgaac gccgggatta ccggcttcac gatcgattcg cggcgcaccc
    29521 ggccgggcga cctgttcatc gcgatccgcg gcgaccggtt cgacgggcat gccttcgtcg
    29581 cgtcagcgct ggcggcgggc gccggcgggg cggtggtgat ggaggcgccg cccgggctga
    29641 cgcccgaggt tctcggcgac cgcctgctga tcgcagtgcc cgacaccacc ctcgggctcc
    29701 aggcgctggc ccgaagcatc cggcgccgct ccggggcccg ggtcgtcgcc gtgacgggga
    29761 gcgcggggaa gaccaccacc aaggagatga tcgcggcgtt cctctcgctg gccgggcacg
    29821 tgatgcggtc gcccggcaac ctgaacaacc acatcggact gccgctctcg ctcctcgagc
    29881 tgcgccacgg cttcgacgcg gccgtcgtcg agctggggat gaaccacgcg ggcgagatcg
    29941 accggctcgt ctcgatcgcc gagcccgacg tgcgggtctg gaccaacgtg gccgaggtcc
    30001 acacagagtt cttcccgtcg atcgaggcga tcgccgacgc caaggccgag gtgctgaacg
    30061 gcgccaccac gggcacggtg ctggtggcca acgccgacga cccgcgggtc gtggcacgga
    30121 tgggctcctt cccgggccgg acggtgacct tcgggttctc gccgggcgcc accgtccgcg
    30181 ccgagcaggt ggaggacgcc ggacttggcg gcatgcgggc gcgcgtcacg acgccctcgg
    30241 ggacgacggc gtggagcgtg gcgctccccg ggcggaacaa cctgctgaac tcgctggcgg
    30301 cagccgcggt cggcctcgag ttcggcgtgc cgcttgccgc gatggccgag gccgcgtcgg
    30361 gcctggcgcc ctccgcgcac cgcggcgagc tggtgcggct cgcccgcggg atcgtcgtgc
    30421 tggacgactc gtacaactcg aacccgcgcg ccctcgaggc cgccctcgac ggggtggatg
    30481 tgctggtgct gtccgactac gc
//
LOCUS       DAOPQX010000005        30076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_1981_length_30076_cov_4.873389, whole genome shotgun sequence.
ACCESSION   DAOPQX010000005 DAOPQX010000000
VERSION     DAOPQX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 30076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30076
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_30076_cov_4.873389"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="PKK95_00575"
     CDS             complement(<1..1770)
                     /locus_tag="PKK95_00575"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOC16727.1"
                     /translation="MPAHDALIPVLHNVATRLRIESIRSTTAAGSGHPSTCCSAADIV
                     AALFFAEMRFDPRHPDDPDNDRFVMSKGHSAPLLYAAWAEAGAFPREELLELRRIDSD
                     LEGHPTPRLPFVDVATGSLGQGLAAGVGIALNARRTGSAYRTYVLLGDGETAEGSVWE
                     AAEAARFQELSNLCAIIDVNALGQSRETAAGHDLDVYVRRFEAFGWRTVTVDGHDLAA
                     LLAAFETARRTTDRPTVLVARTIKGKGVSFIEGKNGWHGKPLKPEEEARALAELRGQF
                     LEEGGGPDVAAPQIQGPPRTTGGPELARPARPSAPPYKAGDKVATREAYGTALVRLGE
                     LDPRIVALDGDVGNSTFSDKFEKAFPQRFYQCFIAEQSMVGTAMGLASRGAIPFAATF
                     AAFFTRAYDFVRMAAISGVGVKMSGSHAGVSIGEDGPSQMGLEDLAMMRAQPNLTVLY
                     PCDAVSTDRLVAEMAYAPGPAYIRTSRPKTPVIYGPDEAFPIGGCKVLRSSDADVATV
                     VAAGVTVFEALKAYEQLRESGTPVRVIDLYSVQPIDRETLRAAAAATKGRVITVEDHY
                     EAGGIGDAVAEATAGVATVRRLAV"
     gene            1922..2929
                     /locus_tag="PKK95_00580"
     CDS             1922..2929
                     /locus_tag="PKK95_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOC16728.1"
                     /translation="MTHRLTLIPGDGIGPEVTRAVVRILAEAGVSIEWEQEEAGLTAV
                     EQLGEPLPQRMLDSVRRTRVALKGPLTTPVGQGFTSVNVGLRKTLDLFVNLRPVFNLP
                     GIAARFGHLDLVLVRENTEDLYSGLEHEVVPGVMVSMKVITRTASMRIARFAFDYARR
                     HGRRRVTTVHKANIMKRGDGLFLACAREVAAEYPEIQHDDRIVDAACMHLVLRPESFD
                     LLLMPNLYGDIVSDLCAGLVGGLGVVGSANLGHDAAVFEAVHGTAPDIAGQDAANPTA
                     LLLSALMMLRHIGEDAAADRIRGALTRVLEAGQVRTRDLGGGATTTAFADAICRELAA
                     G"
     gene            2989..4116
                     /locus_tag="PKK95_00585"
     CDS             2989..4116
                     /locus_tag="PKK95_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOC16729.1"
                     /translation="MGDAISRAVLERDEVVRYLRGGHGEDPRTARERVDKYLAELQTT
                     QRYPLYRALKHPVYPLLRKIERHVEHIDRAVAAASLGRVFWASNHKSHADYLIEPIVL
                     DDAGIRPPVIAAGINLFGGPLGLLHRHVTGAVPIRRNTKDPAYLITLKAYIAELLRRH
                     DILFYPEGGRSYSGELKPPKTGLIHAAMQAEVENLVIVPLAISYELVLEDRILSRQGV
                     KRRQRSFSGELAEMVRDAVGYRSRAFITFGEPILTSGRDAQSRRDMLDLAHTVVDSIG
                     RLYKVTAPAVVAATMRPRTPVRELEDRAAALVARLGEEGANLAVEDGRQAVAEGVPAL
                     ERRGVVACEGDRVRVRSRALVRYYARTIEHLVTPRRSAARP"
     gene            4117..5040
                     /locus_tag="PKK95_00590"
     CDS             4117..5040
                     /locus_tag="PKK95_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOC16730.1"
                     /translation="MLSKALFHALAASRPLERLASRYGMAPGGFARRFVAGETVAEAI
                     EVGRALQARGFLLTLDQLGERVSTLDEADAASRAYVRTAAEVVASGIERNLSVKLSQL
                     GLHLDPAVCTDNLRRILMAAAPQGFFVRVDMEESSTVDTTLAIVKTLREQGFDELGVV
                     LQSALYRSEQDLARMLELRVRVRLVKGAYKEPKPVAYQKKRDVDAAYERMMRRLMTEG
                     NYPALATHDPVLIQRARQFASSRGIGPDRFEFQMLYGVRRDLQTALLSEGCRVRVYLP
                     FGREWFPYYMRRLGERPANLAFVVRSLLRER"
     gene            5248..5565
                     /locus_tag="PKK95_00595"
     CDS             5248..5565
                     /locus_tag="PKK95_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16731.1"
                     /translation="MLFDFSSSMKTNGPHQSLSLSLADTEFQEDRRPGVIATTMSWIQ
                     QGLCGLHGHDALLQYERNRIFLRCTSCGHETPGWDLSRARAAASPAETHATPAAADLA
                     EVA"
     gene            complement(5596..6693)
                     /locus_tag="PKK95_00600"
     CDS             complement(5596..6693)
                     /locus_tag="PKK95_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16732.1"
                     /translation="MRSAFPAWPLAAFAVLAAAVPLAGTIPAGAPHAWPAQESAPQPR
                     QPDPRERHLANLRMLTDGGENAEAYFSFDGQRLIYQATRPGAGCDQIYTMNADGSDQR
                     MVSTGKGRTTCAFFYPDGQSILYGSTHLASPDCPPKPGFERGYVWPIYAGYDIFRARA
                     DGSGLERLTSTPGYDAEATIGRDGRIVFTSVRDGDMELYSMNGDGSDVRRLTRRVGPD
                     GGAFFSPDGRSIVFRGRTLADGPELQDYRSLLKAELWRPTALEVFVMNREGGGLRQIT
                     SAGAASFAPYFHPDGRRIIFSSNMDDPRGRNFDLYLIGVDGQGLERVTFNGTFDGFPM
                     FSPDGKRLVFASNRWSTHEGDTNVFIADWVE"
     gene            complement(6804..6980)
                     /locus_tag="PKK95_00605"
     CDS             complement(6804..6980)
                     /locus_tag="PKK95_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16733.1"
                     /translation="MSEVQEVQEVQGVQKVLVLEVQKVLVRVVQRVLVLEACRAAAPG
                     AKAGAKRALGGGGC"
     gene            7107..7754
                     /locus_tag="PKK95_00610"
     CDS             7107..7754
                     /locus_tag="PKK95_00610"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOC16734.1"
                     /translation="MQTFRRSANTVFVVSLVALVLGVGGLIALAYALQGSPYVTRAFD
                     EREQPVQFSHERHVAGNGIDCRYCHTSVETSAFAGIPPTKTCMNCHSQIFADSPFLEP
                     VRASFRDDRSIAWTRVHDLPDFVYFDHSIHVNKGVGCSTCHGRVDLMPLMFQVETLQM
                     NWCLDCHRNPEKYVRPRDQVFRMDYQPPADQISLGRKLVADYNIRSLTSCSTCHR"
     gene            7774..10794
                     /locus_tag="PKK95_00615"
     CDS             7774..10794
                     /locus_tag="PKK95_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HOC16735.1"
                     /translation="MNDATPNDLAAIRARLEQARGPEYWRSLEELAGTPAFQEALHRE
                     FPQGASELTDPVSRRTLLKLMGASLALAGITACTRQPAEKIYPYVRQPEELVPGRPLF
                     FATAMPLDGYGTGLLVESHEGRPTKVEGNPEHPVSRGSTDAFAQASILGLYDPDRSTA
                     ITYLGEIRPWGAFLTAAKAAVEARRARGGAGVRLLTGTITSPTLGEQIRALTAEFPEL
                     RWHQWDPAGRDQARAGAALAFGEIAETHYQLGQAAVILALDADFLACGPASLRLAREF
                     ADGRRLTGGRREMNRLYAVEPSPTLTGSVADHRLPVPATAIHGFALALAAALGVPGLP
                     QPRDASAHAGWIVPLARDLQAHRGRSVVIVGDEQPAAVHAVAHAINEALGNVGTTVVY
                     TDPVEVSPVDQLASLSELAADMEAGLVETLFILGSNPVYTAPADLEFGRRLEKVKQRF
                     HLGQHLDETAALCQWHVPEAHYLESWGDVRAADGTASLIQPLIAPLHGGRTAHEVLGA
                     LGQQAGQSAHSIVRAYWQARLAPEGGPAFEGQWRRALHDGLIPGTAATARAVKLAVLG
                     DRVAEPPPRQGLEIVFRADPTVHDGRFANNPWLQELPKPHTKLTWDNAALVSPSTAAR
                     LGLANEDLVELRYRGRRVEAPVWVVPGQAADSITVHLGYGRERGGRIAAGPGFDAYRL
                     RTTSAPWAERGLEVAQTGRRYRLASAQNQQMIDARPVVRSATLEEFRRQPGFAREMAH
                     EPPRTLTLYPEQEYKGYAWAMTIDLNTCIGCGACVAACNAENNIPVVGKTEVSRGRRM
                     HWLRVDRYYRGDPENPEAFNQPVPCMHCEKAPCEVVCPTAATVHSAEGLNDMVYNRCV
                     GTRYCSNNCPYKVRRFNFLLYQDWNTPTLKMARNPDVTVRSRGVMEKCTYCVQRINQA
                     RVEAEKEGRPIRDGEVVTACAAACPTKAIVFGDQNDAASAVAKNKAEPLNYSLLGDLN
                     TRPRTTYLAAVRNVNPEMPGARAGGGDDHHEAGR"
     gene            10801..12216
                     /gene="nrfD"
                     /locus_tag="PKK95_00620"
     CDS             10801..12216
                     /gene="nrfD"
                     /locus_tag="PKK95_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOC16736.1"
                     /translation="MASASHPHPPADPPASKGAATPESAFIAPGHTFGTITDKISAIV
                     LTRRTPVGWLLGFAIAFGLVMLFLMAVTWLLLKGTGIWGVNIPVGWGFAIINFVWWIG
                     IGHAGTLISAILLLLHQHWRTSINRFAEAMTLFAVACAGMFPILHTGRPWLAYWLLPY
                     PNSMGLWPQFRSPLIWDVFAVTTYATVSALFWFVGLVPDLATLRDRATSRATRTIYGV
                     LALGWRGSARHWARYESAYLLLAGLATPLVLSVHTVVSFDFAISVLPGWHTTIFPPYF
                     VAGAIYSGFAMVLTLAIPIRAAYGLKDFITLRHLDNMAKVMLATGLIVAYGYAMEAFI
                     AWYSGNFFERFMYWNRLTGPYWPVFWALIATNIVIPQLLWLKKVRLNVPLLFGIALVI
                     NTGMWLERFVIVVTSLHRDFLPSSWGMYYPTMWDWFTYVGTIGLFVAMLFLFIRFLPM
                     ISIFEMRTLLPESKVKDSAHA"
     gene            12233..12787
                     /locus_tag="PKK95_00625"
     CDS             12233..12787
                     /locus_tag="PKK95_00625"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOC16737.1"
                     /translation="MSHAEDPHAPRLHGLVAEFDSPDALVAAGRRAVQEGYRELDAYT
                     PFPIEALHEAMGFDRTRVPLVVLIGAIIGAVAGYGLQYWASTIAYPLNVGGRPLHSWP
                     AFIVPTFETTVLFAALAAVFGMLALNRLPQPYHPIFNTPRFALASRDRFFLAIEARDP
                     KFDLVATRRFLESFNPREVTEVEE"
     gene            12777..13376
                     /locus_tag="PKK95_00630"
     CDS             12777..13376
                     /locus_tag="PKK95_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOC16738.1"
                     /translation="MRNDTALGALVVFCALAAGGCRLDMHDQPKYTPLRQSDFFADGR
                     AARPLVEGTVARGLLNEDELLISGKTDGKLATEFPFPVTAEVVARGRERYDVFCSPCH
                     DRTGAGNGMIVRRGYRAPPSFHQDRLRQAPPGHFVDVMTNGFGAMPDYRSQVPPRDRW
                     AITAYIRALQLSANATLSEVPPAEREKLAPGTGPSGEPR"
     gene            13373..14545
                     /locus_tag="PKK95_00635"
     CDS             13373..14545
                     /locus_tag="PKK95_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16739.1"
                     /translation="MNAHDSVPELAGLRRAALAAAAAGALVTVAGAFVDPTQFFRSYL
                     MAWVYWAGVALGCLGLLMVYHLTGGGWGVAIRRLLEAAIGTLPAAAVLFVPLLFGLGA
                     IYVWTDRAAVLADHVLSQKAAYLNVPFFIARTAIYFLVWLALAHFLDRWSRRQEETAD
                     PRPGERMKRLSGGGLVIYGLAMTFAAFDWIMSLDPHWYSTIFGMIFIADSALGALAFV
                     LVISYLLARGGAFGRVLAPSILNDLGNLLLAFVMVYAYVSFSQLLIIWSANLPEEITW
                     YLRRIEGGWHLLAIALAVFYFAVPFMVLLSRGNKRQPQRLALIAGGVLAARLLSVFFL
                     IAPAFHEHGLAAHWLDAAALVGLGGTWLALFVWRLSSRPLLAHGDPELAPALARRH"
     gene            14645..15118
                     /locus_tag="PKK95_00640"
     CDS             14645..15118
                     /locus_tag="PKK95_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16740.1"
                     /translation="MAHGHTHDEYVPPPGMKYETRDLSTRVVVIFGVSLLVAAIVIHI
                     AVWWLYAWMGRANESAYARQFPLAPVGAPQLPPAPRLQTQPREELKQMRRDEDGLLSG
                     YGWADPNAGRVRLPIDRAMQLVVEQGLPSRAAGPAAPSGLPQDSSSGRTYAPAGK"
     gene            15122..15970
                     /locus_tag="PKK95_00645"
     CDS             15122..15970
                     /locus_tag="PKK95_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOC16741.1"
                     /translation="MRHHLAAAAVALSLAAVPAFAQYNGVPRPGMMTKGDKPAGITPN
                     ELKKVEFAQRLDVQVPLDLPFTDEAGRAVTLAQYFDGRPVILALVYYECPMLCVQALN
                     GLVKSLKTLSLEPGRDYEVVTLSFDPRETPAQALEKKEHYVERLGREGGAAAWHFLTG
                     DDAAIRLLTDAVGFHFVYDEGTGQYAHPTGIVVLTPEGRTSKYIYGLDYGPRDLRLAL
                     VEAAGGKIGTPVDSLLLYCFHYDPEQGKYGLVLMSVLRLAGVVTVTCIGAFILLMWRR
                     EKRARK"
     gene            15974..17026
                     /gene="coxB"
                     /locus_tag="PKK95_00650"
     CDS             15974..17026
                     /gene="coxB"
                     /locus_tag="PKK95_00650"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOC16742.1"
                     /translation="MNWSLPLFPTGASTMAGKVDLLYFVLIAVSGLAVAVISLLIIAF
                     GVRYRRRAPDQVGAPVIPSLALEIGWTAVPLVVGLVLFAWGANVYFAQARPPVETLDV
                     YVVGKQWMWKFQHPGGQREINELHVPVGRAVRLTAASEDVIHSFFVPAFRVKADVIPG
                     RYTTLWFNATKPGEYHLFCAEYCGTQHSGMIGKVVVMEPNAYQAWLGGGAAEGSMASQ
                     GQKIFQDLACHTCHLDSGQGRGPTLVGLYGSQVGLASGQVVTADDAYIRESVLSPAAK
                     VVAGFQPVMPTFQGLVSEEQLLALIEYVKSLQAKPQGTPATQAPAGQQPAAATPAAPQ
                     PRAPGPAGRNPGGDRN"
     gene            17031..18644
                     /gene="ctaD"
                     /locus_tag="PKK95_00655"
     CDS             17031..18644
                     /gene="ctaD"
                     /locus_tag="PKK95_00655"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HOC16743.1"
                     /translation="MDSLTLPRTHYANAEYGLRSWLLTTDHKRIALLFLGSISAFFLI
                     GGLFAMLIRLELLTPPGDMVGADTYNKLFTMHGIVMIFMFLIPSIPAVFGNFFVPIMV
                     GAKDLAFPRLNLLSWYIFMIGGLFTLAAIALGGVDTGWTFYTPFSTHASQSNVMVAAV
                     GVFIMGFSSILTGLNFIVTIHTMRAPGLTWFRLPLFIWAMYATSLINVLGTPVLAISL
                     VAVALERALHIGIFDPAYGGDPVLFQHLFWFYSHPAVYIMVLPSMGVVSELVATFSRK
                     NIFGYTFVAFSSLAIAVFGFLVWGHHMFVAGMSVYGAMIFSILSFMVAVPSAVKVFNW
                     TATLYKGSISYETPMLYALGFIGLFAVGGLTGLFLATLAVDVHVHDTYFVVAHFHYIM
                     VGGALMGYLGALHYWWPKMTGRMYPDLIGRIAAVTVFIGFNLTFFPQFVLGYLGMPRR
                     YHAYAEEFQALNVLSTAGASILAVGFLIPAFYLLWSLKYGKVAGANPWGSVSLEWTTP
                     SPPPTFNFDETPVIAGPAYDYREPEAKRA"
     gene            18637..19338
                     /locus_tag="PKK95_00660"
     CDS             18637..19338
                     /locus_tag="PKK95_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HOC16744.1"
                     /translation="MPDAAFDPRAVPGADAHAAALHSPALKHHFDDLGQQKEAATLGM
                     WLFLATEVLFFGGLFAAYLLYRVWYPDVFGEASRSLDITLGSVNTLVLIGSSLTMALA
                     VHAAATGQRRTLMIFIALTMLLGAVFLGIKGVEYYDKIVHHHVPGPSFHFEGPTPERA
                     QLFFSLYFAMTGLHAFHMVIGLGLMTVILLMAWRGRFSATWYTPVEMSGLYWHFVDII
                     WIFLFPLLYLVDRHS"
     gene            19353..19640
                     /locus_tag="PKK95_00665"
     CDS             19353..19640
                     /locus_tag="PKK95_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOC16745.1"
                     /translation="MSHVIPRRIYYVIFAVLVVLTLVTTEVAFFDFGLMNPVIALTIA
                     VVKATVVVLYFMHMRYSSRLTMVIGAAGIFWLGILLALTMSDYLARGAGTY"
     gene            complement(19650..20648)
                     /gene="lpxD"
                     /locus_tag="PKK95_00670"
     CDS             complement(19650..20648)
                     /gene="lpxD"
                     /locus_tag="PKK95_00670"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOC16746.1"
                     /translation="MTLSAAEIAGLVNGRVIGDPGTPVSGIATAATARVTDLAFVEDA
                     RSLDEALASAAGVLVAGPFAADASFHGALVVCDQPRLAFARAGRILSRPASLPPGVHQ
                     TAIVHPAAILGRGVAVGPNAVVDEGAEVGDLVQLGAGCHVGAGVRIGSGTVLRSHVVV
                     YPGTRIGAGVVVHAGTVLGSDGFGYVRDAATGRYEAFPQVGRLVIEDDVELGTLCTVD
                     RGALGETVIGRGTKLDNQVHVAHNVRIGRDVVIAAQTGIAGSSVIEDGAIIAGQVGIA
                     DHVTVGGGAILGAQCGVPTGKVLRGPGTLFWGTPARPIKQVLKELAVLARLAKKKA"
     gene            complement(20645..22456)
                     /locus_tag="PKK95_00675"
     CDS             complement(20645..22456)
                     /locus_tag="PKK95_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16747.1"
                     /translation="MNESEQEKQDVVAQWSFDTRPILGRFHLWLEDVEVEWTRGERVL
                     EFTRNLSFLDGRMERLLAMTGAVTAIGTQVFGHPGAGARLDRAGLNQVKKDADAISAY
                     VMSESLWYLSRHLPENHAIMVCLGEGLMPKAGETPEMGSNPQIGFGRVYARPEIARHL
                     DRLVVRLLNDPGYGWDGFYREIRESGITVWGAAIDTLENTSRFAKGSPTGPMTILHLF
                     NQPLQISRPYEGYVGMLVLPKEVVAQAADRAILLDFRTPRRLVMEAIEATWPGIRRDR
                     VHVWTLGGKSREGRIGRLWKSWQELGAHLVEEGWELPGGMAAFTESGTYAPTYRIGTW
                     LDEAGERHLFLCDGYAASAEAVQAASIAPILGLDASMATFTSRFDLPYDEEQDVMRLD
                     PAAPDFASRLAALASDSLGDAEVDDYRRMILEARDAGMLGDRPTLTADHFFPEKRWEV
                     LALSGYMGPDPYSGAPGVEEVSPGVYRATVRMVSKHGDKRVALTLRLGETFEQSRLVF
                     SPLLNRFLAGEPFETRPVKISDSGRIRNELQTLCAEALEFLDAKTIRLHFDRIPPEVI
                     SPEKQKALLEVLRWYKRRHPIWFSWLEMTPAGEAARR"
     gene            complement(22486..24171)
                     /locus_tag="PKK95_00680"
     CDS             complement(22486..24171)
                     /locus_tag="PKK95_00680"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOC16748.1"
                     /translation="MSPTSAETPVDFIRAIIREDLESGKHDTVRTRFPPEPNGYLHIG
                     HAKSICLNFGVAEEFGGTCNLRFDDTNPAKEDVEYVDSIIDAVHWLGFDWGDRLYHAS
                     DYFDRIYEYTLGLIRDGKAYVDDLSADEIREYRGTLTEPGRESPWRNRSVEENLELFE
                     RMRRGEFADGERVLRAKIDMASPNLNLRDPVIYRIRHASHHRTGDKWCIYPMYDYTHA
                     ISDAIEHITHSLCTLEFEDHRPLYDWTVENLPVPSRPRQIEFARLNLTFTVTSKRKLL
                     QLVQDGVVSGWDDPRMPTIVGLRRRGYTPESIREFASRVGVAKRENLVDVALLEDALR
                     DDLNRRAPRAMAVLRPLRVVIENYPEGQVEEIEIPVNPEDASMGVRKVPFSRELYIEQ
                     DDFREVPPPKYFRLSPGREVRLRGTYFLTCERAVKDADGNVIELRCRYDPATRGGASP
                     DGRRVKATIHWVSAAHARDAEVRLYDRLFSVPDPHDADEGRDWKSYLNPGSLTVLKGC
                     KIEPGLDAAPAFSHYQFERQGYFCVDPDSTPGRPVFNRSVPLRDSWARLAASR"
     gene            complement(24298..24723)
                     /locus_tag="PKK95_00685"
     CDS             complement(24298..24723)
                     /locus_tag="PKK95_00685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOC16749.1"
                     /translation="MSEVAERMFPHCYVCGQENARGLRVAFAPHPGGGCRAEYVAREE
                     HVGWPEVIHGGLLFTLMDEAVAWAVIYAGLHGVTARAEARFREPVRVGTPLVVRGWLV
                     DPPRRVTRARAELRAGSEEGPLVAELDALMAITKDTTIA"
     gene            complement(24757..25257)
                     /locus_tag="PKK95_00690"
     CDS             complement(24757..25257)
                     /locus_tag="PKK95_00690"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC16750.1"
                     /translation="MFTKMADFKAGWDVEREGTLKLLRALTDASLAQEVAPGSRTLGR
                     IAWHMVASVGEMARHAGLTIDAPPDDAPVPATAREFVETYEKSSAALPAAVEKAWSEA
                     DLQGTIEMYGEKWTRGQALAVLIAHQTHHRGQMTVLMRQAGLPVPGVYGPSREEWTAY
                     GMPAQA"
     gene            complement(25332..26798)
                     /locus_tag="PKK95_00695"
     CDS             complement(25332..26798)
                     /locus_tag="PKK95_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOC16751.1"
                     /translation="MADARDVLAALEDDTDPSVGVAFARLVADYFRDTRGGAGPVSTP
                     LTPAELERRFDEALPVSGRPIEEVLARIERDILPDCNRLMHPRAMGHQVSAPVAASVW
                     TESLTAALNQSCAVWEMSPVGTMIEARVIRWMCGLAGMPAESGGTFTSGGTEANFAGL
                     LAARQALMPDAWARGVGPRPPAVVCGEHAHYSVARAVGELGIGTDNVVLVPTGSDWGM
                     DTIALTRVLDRLRAEGRVVMAVVATAGSTATGAFDDLEAIGRACEERGLWFHVDGSHG
                     ASALLSPRHRERLAGLGRASTIAWDPHKMMLMPLTASVVLARREDDLERAFSQSAPYL
                     FHARQGRNWDQGLRSFSCSRRADALKVWVALQRYGAAGLGALYERLCETCSQLHDLVV
                     SRPAFRAAHVPASNILCFRFVGDRGEDDPGLDDLNLRLRSTYNQGGDGWITTTVLGGR
                     RVLRATIMNPRTETGDLVRLLDGLERTAGTLAGRRGGC"
     gene            complement(26868..27443)
                     /locus_tag="PKK95_00700"
     CDS             complement(26868..27443)
                     /locus_tag="PKK95_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOC16752.1"
                     /translation="MRRSIFAAALLLIVALAAPAAANPIAFVSGPSPVAIVSGILTVT
                     GPEVNPGDFGLEATTGSMFTGNWPGFSQRTSNGQVPGTWPVLGPPAQVPSRGGSTGGS
                     PPAIPFVGQLLGYPPSTTMPPGLLVAVIVLTGVPASQIPVAGGLFVPPGLQGASLDGA
                     NVPEPGTILLLSSGLAGLVVARRRRKKGARA"
     gene            complement(27550..28041)
                     /locus_tag="PKK95_00705"
     CDS             complement(27550..28041)
                     /locus_tag="PKK95_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HOC16753.1"
                     /translation="MPLTVRRAARWALPVVAVLLAIAQFVPVDRSNPPSDPQQSLAAT
                     AGPPAAVVAVLDRSCRDCHSNETRWPWYSRVAPISWLVAGDVEEGRSHMNFSEWALLD
                     NRRQARRLEEICEEITTGAMPDPKYVLLHPEARLSAEEITAVCDWTRREAEALGAKPP
                     AGR"
     gene            28135..29613
                     /locus_tag="PKK95_00710"
     CDS             28135..29613
                     /locus_tag="PKK95_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOC16754.1"
                     /translation="MAHLTAARLLVLAALGLAIAGLSASTREAAPDDSSPAPVSPVPS
                     ERQLRWHELEYYGFLHFGPNTFTNKEWGYGDEAPAIFNPAALDARQWARVARDAGMKG
                     LIITAKHHDGFCLWPSRFTRHSVAASPWRQGRGDVVRELSRACREFGLEFGVYLSPWD
                     RNHAEYGRPAYLAYYRDQLRELLGGYGPVFEVWFDGANGGDGFYGGARETRRIDNRTY
                     YDWPVTWNLVRALQPGAVMFSDAGPDVRWVGNEKGIAFESTWYGLDRSATYPGDPRYA
                     KELARGRIDGADYVPPEVDVSIRPGWFHHPEQDEKVKSVEELLEIYYSSIGRGANLLL
                     NIPPDRRGLIPEGDVARLMEFRRAVEDAFAKDLARRAVATASTQRGQGPQFGPSRAND
                     GDPATYWATSDGVTTGSVELSWKAPVAFERIVLQEAIALGQRVQGWRVEAEVDGAWQT
                     LAEGESIGYKRIARVGRTTASRLRVAITAARACPTLATIGVY"
     gene            complement(29618..>30076)
                     /locus_tag="PKK95_00715"
     CDS             complement(29618..>30076)
                     /locus_tag="PKK95_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOC16755.1"
                     /translation="SLPLLSLKTLRDHRDDSVHVWMACASAQVFTAFGLAALLLATLG
                     VYGVKAYLVSRRTREIGIRVALGASRLDVLALVLGDGLWLTVGGLLVGVVLALGLSKV
                     LAAWVYGVTGIEPIALLAASSVLGGAALVACYLPARRAMKQAPSMALRME"
BASE COUNT         4427 a        10795 c        10227 g         4627 t
ORIGIN      
        1 cacggcaagc cggcgcaccg tcgccacacc ggcggttgcc tcggccaccg cgtcgccaat
       61 cccccccgcc tcgtagtgat cctccacggt gatcacccgc ccctttgtcg cagcggcggc
      121 cgcccgcagg gtctcgcggt cgatcggctg caccgagtag aggtcgatca cacgcaccgg
      181 cgtgcccgac tcgcgcagct gctcgtaggc cttcaacgcc tcgaacacgg tcacgccggc
      241 cgccaccacg gtcgccacgt cggcgtcgct gctacgcagc accttgcagc cgccgatcgg
      301 gaacgcctcg tcggggccgt agatgaccgg cgtctttggc cggctcgtcc ggatgtaggc
      361 gggcccgggg gcgtacgcca tctcggcgac gaggcggtcg gtgctcaccg cgtcgcacgg
      421 gtagagcacg gtgaggttgg gctgggccct catcatggcg aggtcctcga gccccatctg
      481 cgaggggccg tcttcgccga tggacacgcc ggcgtgcgac cccgacatct tcacgcccac
      541 cccgctgatg gccgccatcc gcacgaaatc gtaggcccgg gtgaagaacg ccgcgaaggt
      601 ggcggcaaac gggatcgctc cacggctcgc caaccccatg gcggtcccca ccatcgactg
      661 ctcggcgatg aagcactggt agaagcgctg cgggaacgcc ttctcgaact tgtcgctgaa
      721 ggtggagttg cccacgtcgc cgtcgagggc gacgatgcgc gggtcgagct caccgaggcg
      781 gacgagcgcc gtgccgtagg cctcgcgggt ggccaccttg tcgcccgcct tgtagggggg
      841 cgcggagggc cgggcgggac gggccagctc ggggccgccc gtcgtgcggg gcggcccctg
      901 gatctgcggc gccgcgacgt cgggcccgcc gccttcctcg aggaactgcc ccctgagctc
      961 ggcgagcgcc ctcgcctctt cctccggctt cagcggcttg ccgtgccagc cgttcttccc
     1021 ctcgatgaag gacaccccct tgcccttgat cgtgcgggcc accagcacgg tcggccggtc
     1081 ggtggtccgg cgcgcggtct cgaaggcggc cagcagcgcc gccaggtcgt ggccgtccac
     1141 cgtcacggtg cgccacccga acgcctcgaa gcgacggacg tacacgtcga gatcgtgccc
     1201 ggccgccgtc tcgcggctct ggccgagggc gttcacgtcg atgatggcgc acaggttcga
     1261 cagttcctgg aagcgcgccg cctcggccgc ttcccacacc gacccctccg cggtctcacc
     1321 gtcgcccagc agcacgtagg tccggtaggc cgagccggtc cgcctggcgt tgagcgcgat
     1381 gcccaccccg gcggccagcc cctggccgag cgacccggtg gccacgtcca cgaacggcag
     1441 gcgcggcgtg gggtgcccct cgaggtcgga atcgatccgg cgcagctcga gcagctcctc
     1501 ccgcgggaag gcgcccgcct cggcccaggc cgcgtacagc agcggcgcgg agtgcccctt
     1561 cgacatcacg aaccgatcgt tgtcgggatc gtccgggtgc cgggggtcga agcgcatctc
     1621 ggcgaagaac agggcggcca cgatgtcggc cgccgagcag caggtcgagg ggtggccgct
     1681 gccggccgcg gtcgtcgagc ggatggactc gatccggagc cgggtggcga cgttgtgcag
     1741 gaccgggatc agggcatcgt gcgcaggcat cagcaggacc tccagaaaac cagctgtcca
     1801 ttatctccgg agacgccgcc ctggtgaaac ggccggcacg gaatttccag cccgccgcgc
     1861 tgcagggccg ccggcggggc ggaacgcgaa aatccgcccc gaacggttta tgctcatgcc
     1921 tatgacgcac cgcctcaccc tcatccccgg ggacgggatc ggccccgagg tcacgcgggc
     1981 cgtcgtacgc atcctggcgg aggccggggt cagcatcgag tgggagcagg aggaggccgg
     2041 cctcacagcc gtcgagcagc tcggcgagcc gctgccgcag cggatgctcg actcggtccg
     2101 gcgcacgcgc gtcgccctga aaggcccgct cacgacgccc gtggggcagg gcttcaccag
     2161 cgtgaacgtg ggcctgcgca agacgctcga cctgttcgtg aacctccggc ccgtgttcaa
     2221 cctccccggc atcgcggcgc gcttcggcca cctcgacctg gtgctggtgc gcgagaacac
     2281 cgaggacctc tactcgggcc tcgagcacga ggtcgttccg ggggtgatgg tgagcatgaa
     2341 ggtgatcacg cggaccgcct cgatgcggat cgcgcgcttc gccttcgact acgcgcgccg
     2401 ccacggccgc cgccgcgtga ccaccgtgca caaggcgaac atcatgaagc gcggcgacgg
     2461 gctcttcctg gcctgcgcgc gcgaggtggc cgccgagtac ccggagatcc agcacgacga
     2521 ccggatcgtg gatgccgcgt gcatgcacct ggtgctgagg cccgagagct tcgacctgct
     2581 gctgatgccg aacctgtacg gcgacatcgt gtccgacctg tgcgcgggcc tggtcggcgg
     2641 cctcggcgtg gtgggcagcg cgaacctcgg ccacgacgcg gcggtgttcg aggccgtgca
     2701 cggcacggcg cccgacatcg cgggccagga cgcggcgaac cccaccgccc tcctgctctc
     2761 ggcgctgatg atgctgcgcc acatcggaga ggacgccgcc gccgaccgga ttcgcggggc
     2821 gctcacgcgc gtgctcgagg ccgggcaggt caggacccgc gacctgggcg gaggcgcgac
     2881 cacgaccgcg ttcgccgacg ccatctgccg cgagctggcc gcgggctgac gcggcgcgcc
     2941 cgcaggggcg ggctcaacgt cgcggggcct ggtgtatagt tgcgcgcaat gggcgacgcc
     3001 atcagccggg ccgtgctcga gcgcgacgag gtcgtccgct acctgcgcgg agggcacggc
     3061 gaggatccac gcaccgcccg cgagcgggtg gacaagtacc tcgcggagct gcagaccacc
     3121 cagcgctacc ccctctaccg ggcgctcaag cacccggtct atcccctcct gcgcaagatc
     3181 gagcgccacg tcgagcacat cgaccgggcg gtggcggccg ccagccttgg acgggtgttc
     3241 tgggcctcga accacaagag ccacgccgac tacctgatcg agccgatcgt tttggacgac
     3301 gccggcatcc gtcccccggt aatcgcggcc ggcatcaacc tgttcggagg cccgctgggg
     3361 ctgctgcacc gccatgtcac cggcgccgtg cccatccgcc ggaacaccaa ggacccggcg
     3421 tacctgatca cgctcaaggc ctacatcgcc gaactgctgc ggcgccacga catcctcttc
     3481 tacccggagg gcggccgcag ctacagcggc gagctgaagc ctcccaagac ggggttgatc
     3541 cacgcggcga tgcaggcgga ggtcgagaac ctggtcatcg tgcccctcgc gatctcgtac
     3601 gagctggtgc tcgaggatcg gatcctctcg cgacaggggg tcaagcggcg ccagcggtcg
     3661 ttctcgggcg aactggccga gatggtccgc gatgcggtcg gctaccgctc gcgcgccttc
     3721 atcaccttcg gggaaccgat cctgacctcg ggccgcgacg cgcaatcgag acgcgacatg
     3781 ctcgacctgg cccacacggt cgtcgactcg atcggccgtc tctacaaggt cactgcgccg
     3841 gcggtggtgg ccgcgaccat gcgcccgcgg acccccgtgc gcgagctgga agaccgggcc
     3901 gcggccctgg tggctcggct gggggaggaa ggggcgaacc tcgccgtcga agacggccgc
     3961 caggcggtcg ccgaaggggt gcccgccctc gagcgccggg gcgtggtcgc ctgcgagggc
     4021 gaccgggtgc gcgtgcgcag ccgagcgctg gtccgctact acgcgcgcac catcgaacac
     4081 ctggtcaccc cccgccgcag cgccgcgcgg ccctgaatgc tgtcgaaggc cctgtttcac
     4141 gcactcgccg ccagccggcc cctcgaacgc ctcgcctcca ggtacggcat ggcgcccggc
     4201 ggcttcgcgc gccgcttcgt cgcgggcgaa accgtggcgg aggccatcga ggtcggccgc
     4261 gcgctgcagg cccgcggctt cctcctgacg ctcgaccagc tcggcgaacg ggtctcgacc
     4321 ctcgacgagg ccgacgccgc ctcgcgggcc tacgtccgca cggctgccga ggtcgtggcc
     4381 tcgggcatcg agcgcaacct ctcggtcaag ctgtcgcagc tcgggctcca cctcgatccg
     4441 gccgtctgca ccgacaacct gcggcgaatc ctcatggcgg ccgcccccca gggcttcttc
     4501 gtccgagtgg acatggaaga gtcctctacg gtggacacca cgctggccat cgtcaagacg
     4561 cttcgcgagc aggggttcga cgagctcggt gtggtgttgc agtcggcgtt gtaccggagc
     4621 gagcaggacc tcgcccgcat gctcgagctt cgcgtccgcg ttcgcctcgt gaaaggggcg
     4681 tacaaggaac cgaagccggt ggcctaccag aagaagcgcg acgtggacgc ggcctacgag
     4741 cggatgatgc ggcggctcat gaccgagggg aactaccccg ccctggcaac ccacgacccc
     4801 gtgctgatcc agcgcgcccg ccagttcgcg tcgtctcgcg ggatcggccc cgaccggttc
     4861 gagttccaga tgctgtacgg cgttcgccgc gaccttcaga ccgcccttct ctcagaaggc
     4921 tgccgcgttc gcgtctacct gccgttcggg cgggagtggt tcccctacta catgcggcgg
     4981 ctcggggaac gccccgccaa cctcgcgttc gtcgtccgga gcctcctccg cgagcggtaa
     5041 cggggtccgt gcccggcgtg ttgcacggga gcggccaccg tggaagttgt ccttcctcta
     5101 tacagagggt gcccgtttct gcatcgttca cggactgccg aattcacact tctgcaatcc
     5161 cgtcctctgc cggacagccc ttcgccggtt tgacgctttc ccacgcccaa gtcttgccca
     5221 aagatcgaga gcgagctcat ccttggcatg ctctttgatt tcagctcgag catgaagacc
     5281 aatggccccc accagtcgct ttcgttgtcg ctggccgaca ccgagtttca ggaagaccgg
     5341 cgacccgggg tgatcgccac caccatgtcg tggattcagc agggcctgtg cgggctccac
     5401 ggccacgatg ccttgctgca gtacgagcgg aaccggatct tcctgcgctg cacgtcctgc
     5461 gggcacgaga cccccggctg ggacctctcg cgcgcgcgcg cggccgcctc gccggccgaa
     5521 acccacgcaa cgccggccgc ggccgatctc gccgaggtgg cgtagcggac cgcctcgttc
     5581 agcggcgtgc cagggttact cgacccagtc cgcgatgaac acgttggtgt cgccctcgtg
     5641 cgtcgaccag cggttcgatg cgaacaccag ccgcttgcca tcgggcgaga acatcgggaa
     5701 gccgtcgaag gtcccgttga aggtgacccg ttcgagcccc tggccgtcga cgccaatgag
     5761 gtagaggtcg aagttccgcc cgcgcgggtc gtccatgttc gacgagaaga tgatccgccg
     5821 cccatccggg tggaagtagg gggcgaagct ggccgccccg gcgctggtga tctggcgcag
     5881 gccaccccct tcgcggttca tcacgaacac ctcgagggcc gtcggccgcc acagttccgc
     5941 cttcagcagc gagcggtagt cctggagctc ggggccgtcg gccagggtgc gtccgcggaa
     6001 gacgatcgac cgcccgtcgg gggagaagaa cgccccgccg tccgggccga cgcgccgcgt
     6061 caggcggcgg acgtccgacc cgtcgccgtt catcgagtac agctccatgt cgccgtcgcg
     6121 cacgctggtg aagacgatcc gcccgtcgcg cccgatggtg gcctcggcgt cgtacccggg
     6181 cgtcgaggtc aggcgctcga ggccgctccc gtcggcgcgg gcccggaaga tgtcgtaccc
     6241 cgcgtaaatc ggccagacgt agccgcgctc gaaccccggc ttgggcgggc agtcgggcga
     6301 tgccaggtgt gtcgagccat agaggatcga ctggccgtcc gggtagaaga aggcacaggt
     6361 ggtgcggccc ttgccggtgc tgaccatccg ctggtcgctg ccgtccgcgt tcatcgtgta
     6421 gatctggtcg cagccggccc cgggcctggt ggcctggtag atcaggcgct ggccgtcgaa
     6481 cgagaagtag gcctcggcgt tctcgccgcc gtcggtcagc atccggaggt tcgcgaggtg
     6541 acgctcacgg ggatcgggct gccggggctg cggcgcagac tcctgggcgg gccaggcgtg
     6601 gggagcgccg gcgggaatcg tgcctgccag aggcacggcc gcggcgagaa cggcgaacgc
     6661 ggccaggggc cacgcaggga acgcgctgcg caatcgatcc tccttgctgg ggtagaccct
     6721 ctcccacctt agcacacgcc ctccccaaac gcgccagctc ttcccccccg cacttacgaa
     6781 cgcagccaaa ggtttcagca ccttcagcac ccgcctccgc caagcgctcg cttcgctccc
     6841 gccttcgcgc caggcgctgc ggcgcggcag gcctccagca ccagcaccct ctgcaccacc
     6901 cgcaccagca ccttctgcac ctccagcacc agcaccttct gcaccccctg cacctcctgc
     6961 acctcctgca cctccgacac gacttcccgt ccacctctga accgtagtaa ccttgatccg
     7021 ggtattcaca acgaagcgag cgattcgcgg aggcccccgc ttcccccggc ggcgtccgcg
     7081 gctccaggtc ttggaagacg cggacgatgc agaccttccg tcgaagtgcc aacaccgtct
     7141 tcgtcgtcag cctcgtcgcc ctggtcctgg gcgtcggggg gctgatcgcc ctcgcctacg
     7201 ccctccaggg ctccccctac gtcacccgcg cgttcgacga gcgcgagcag cccgtccagt
     7261 tcagccacga gcggcacgtc gctggcaacg ggatcgactg ccgctactgc cacacctcgg
     7321 tcgagacgtc cgccttcgcg ggcatcccgc ccacgaagac ctgcatgaac tgccattcgc
     7381 agatcttcgc cgacagcccg ttcctcgagc cggtgcgcgc cagtttcagg gatgaccgct
     7441 ccatcgcctg gacccgtgtc cacgaccttc ccgacttcgt ctacttcgac cacagcatcc
     7501 acgtaaacaa gggggtcggc tgctccacgt gccacgggcg cgtcgatctg atgcccctga
     7561 tgttccaggt ggagacgttg cagatgaact ggtgcctgga ctgccaccgc aaccccgaga
     7621 agtacgtccg gccgcgcgac caggtgttcc gaatggacta ccagcccccg gccgaccaga
     7681 tctcgctcgg gcggaagttg gtcgctgact acaacatccg gagcttgacc agctgctcca
     7741 cgtgccaccg gtaaccgctg tccacgcgcg acgatgaacg acgccacccc caacgacctc
     7801 gccgccattc gcgcccgcct cgagcaagcg cgcggacccg agtactggcg gagcctcgag
     7861 gaactggccg gcaccccggc cttccaggag gccctccacc gggagttccc ccaaggcgcg
     7921 tccgagctga ccgatccggt gagccggcgc accctgctca agctgatggg cgcctcgctc
     7981 gcgctggcgg gcatcacggc gtgcacacgg cagccggccg agaagatcta cccctacgtg
     8041 cgccagcccg aagagctcgt gccgggcaga ccgctgttct tcgccaccgc gatgccgctc
     8101 gacggctacg gaacggggct tctcgtcgag agccacgagg ggcggccgac caaggtcgag
     8161 ggcaatccgg aacacccagt cagccgcgga tcgaccgacg cctttgcaca ggcctccatc
     8221 ctcggcttgt acgatcccga ccgctcgacg gccatcacct acctcggcga gatccggccg
     8281 tggggcgcct tcctgaccgc cgcgaaggcg gcggtggagg cccggcgcgc acggggcgga
     8341 gcgggcgtcc ggcttctcac cggcaccatc acctcgccca ccctgggcga gcagattcgg
     8401 gcgctgaccg ccgagttccc ggagcttcgg tggcaccagt gggacccggc cggacgagac
     8461 caggcgcgcg cgggcgccgc gctggcgttc ggcgagatcg ccgagacgca ctaccagctc
     8521 gggcaggcgg ccgtcatcct cgctctcgac gccgatttcc tcgcttgcgg cccggcgagc
     8581 ctgcgcctgg cccgggagtt cgccgacggg cgccggctga ccggagggcg ccgcgagatg
     8641 aaccggcttt acgcggtcga gccgtcgccg acgctgacgg gctcggtcgc ggatcaccgc
     8701 ctgccggtgc cggccaccgc gatccacggc ttcgcgctgg cgctcgcggc cgcgttgggc
     8761 gtgccggggc tgccgcagcc gcgcgacgct tcggcgcacg ccgggtggat cgtgccgctc
     8821 gcccgcgacc tccaggccca ccgcggacgc tcggtcgtca tcgtcgggga cgaacagccg
     8881 gccgcggtgc acgcggtcgc ccacgccatc aacgaggcgc tgggcaacgt cgggaccacc
     8941 gtcgtctata ccgaccccgt cgaggtgtcg ccggtcgacc agctggcgtc gctgagcgag
     9001 ctggccgccg acatggaggc cggcctcgtc gagaccctgt tcatcctcgg ctcgaacccc
     9061 gtctacacgg cgccagcgga cctggagttc ggccggcgcc tcgagaaggt gaagcagcgc
     9121 ttccacctgg gccagcacct cgacgagaca gcggctctct gccagtggca cgttcccgag
     9181 gcgcactacc tcgagtcgtg gggggacgtg cgcgcggcgg atggcacggc gtcgctgatc
     9241 cagcccctca tcgccccgct gcacggcggg cgcacggcgc acgaggtgct gggcgcgctg
     9301 ggccaacagg ccggccagag cgcgcactcg atcgtgcggg cgtactggca ggcgcgcctc
     9361 gcccccgaag gggggcccgc gttcgagggt cagtggcggc gggcgcttca cgacgggctg
     9421 atacccggca cggccgcgac cgcccgggcc gtgaagctgg cggtcctcgg agaccgtgtg
     9481 gccgagccgc cgccgcggca agggctcgaa atcgtcttcc gcgccgaccc aaccgtccac
     9541 gatggccgct tcgcgaacaa tccatggcta caggagctgc cgaagcctca cacgaagctg
     9601 acgtgggaca acgccgccct cgtcagcccc tcgacggcgg cgcgcctcgg cctcgcgaac
     9661 gaggacctgg tcgagctgcg gtaccgcggg cgcagggtcg aggccccggt ctgggtggtg
     9721 cccggccagg cggccgattc catcacggtc cacctcggat acggccgtga gcgcggcggg
     9781 cggattgcgg cgggacccgg gttcgatgcc taccggctgc gaaccacctc ggcgccgtgg
     9841 gccgagcggg gcctcgaggt ggcgcagacc ggccggcgct accggctggc gagcgcacag
     9901 aaccagcaga tgatcgacgc ccggccggtg gtgcgctcgg ccacgctcga agagttccgg
     9961 cgccagcccg gcttcgcccg cgagatggcg cacgagcccc ctcgcacgct cacgctgtac
    10021 ccggagcaag agtacaaggg ctacgcgtgg gcgatgacca tcgacctcaa cacctgcatc
    10081 gggtgcgggg cctgcgtggc ggcgtgcaac gcggagaaca acatccccgt cgtcggcaag
    10141 accgaggtga gccgcggccg ccggatgcac tggctgcgcg tggaccgcta ctaccggggc
    10201 gatcccgaga accccgaagc cttcaaccag ccggttccct gcatgcactg cgagaaggca
    10261 ccgtgcgagg tggtctgccc cacggcggcg accgtgcaca gcgccgaggg gctgaacgac
    10321 atggtctaca accggtgcgt ggggacgcgc tactgctcga acaactgtcc ctacaaggtg
    10381 cgccggttca acttcctgct gtaccaggac tggaacacgc ccaccctgaa gatggcgcgc
    10441 aaccccgacg tcaccgtccg cagccgcggc gtcatggaga agtgcaccta ctgcgtgcag
    10501 cggatcaacc aggcgcgggt cgaggccgag aaggagggcc gcccgatccg ggacggcgag
    10561 gtggtcaccg cgtgcgccgc cgcctgcccg acgaaggcga tcgtgttcgg cgatcagaac
    10621 gacgcggcga gcgcggtggc gaagaacaag gccgagccgc tgaactacag cctgctcggc
    10681 gacctcaaca cccggccccg caccacctac ctcgcggcgg tccggaacgt gaaccccgag
    10741 atgccgggcg cccgggcggg cggcggcgac gatcaccacg aggcggggag gtaagggttc
    10801 atggcttcag cgtcgcaccc ccacccgccc gccgacccgc cggcctcgaa gggcgccgcg
    10861 acacccgagt cggcgttcat cgcgcccggg cacaccttcg gcacgatcac cgacaagatc
    10921 agcgcgatcg tgctgacgcg gcggaccccg gtcggctggc tgctggggtt cgcgatcgcc
    10981 ttcggcctgg tgatgctctt cctgatggcg gtcacctggc tgctgctgaa gggcacgggc
    11041 atctggggcg tcaacatccc ggtcggttgg ggcttcgcga tcatcaattt cgtctggtgg
    11101 atcgggatcg ggcatgccgg gacgctgatc tcggcaatcc tcctgctgct gcaccagcac
    11161 tggcgcacct ccatcaaccg cttcgccgaa gcgatgacgt tgttcgcggt cgcctgcgcc
    11221 gggatgttcc caatcctcca caccgggcgt ccctggctgg cgtactggct gctgccctac
    11281 cccaacagca tgggtctctg gccgcagttc cggagcccgc tgatctggga cgtgttcgcg
    11341 gtcacgacct acgccaccgt gtcggcgctg ttctggttcg tcggcctggt gcccgacctg
    11401 gcgacgctgc gcgaccgggc cacgtcgcgg gcgaccagga cgatctacgg cgtgctcgcg
    11461 ctcggctggc gcggctcggc ccgccactgg gcccggtacg agtcggccta cctgctgctc
    11521 gccggcctcg cgacgcccct cgtgctgtcg gtgcacaccg tcgtgagctt cgacttcgcg
    11581 atctcggtgc tgcccgggtg gcacacgacg atcttccccc cgtacttcgt cgccggggcg
    11641 atctactcgg gcttcgcgat ggtcctcacg ctggcgatcc cgatccgggc ggcctacggg
    11701 ctgaaggact tcatcaccct gcgccacctc gacaacatgg cgaaggtgat gctggccacc
    11761 gggctgatcg tcgcctacgg atacgcgatg gaggcgttca tcgcctggta cagcggcaac
    11821 ttcttcgagc ggttcatgta ctggaaccgc ctgacgggac cgtactggcc ggtcttctgg
    11881 gcgctcatcg cgaccaacat cgtgatcccg cagctgctct ggctgaagaa ggtcaggctg
    11941 aacgtgcccc tgctgttcgg gatcgccctg gtgatcaaca ccgggatgtg gctcgagcgc
    12001 ttcgtgatcg tggtcacgag cctgcaccgc gacttcctgc cgtcctcgtg ggggatgtac
    12061 tacccgacga tgtgggactg gttcacctac gtgggaacga tcgggctgtt cgtcgcgatg
    12121 ctgttcctgt tcatccggtt cctgccgatg atctccatct tcgagatgcg gacgctgctg
    12181 cccgagtcga aggtgaagga ctcggcgcac gcctagcggg ggcagagacg agatgtcgca
    12241 cgccgaggat ccgcacgcgc cccgcctcca cggcctggtg gcggagttcg acagtccgga
    12301 cgcgctggtg gcggccgggc ggagggccgt gcaggagggg taccgcgagc tggacgccta
    12361 cacgccgttc ccgatcgagg cgctgcacga ggcgatgggg ttcgaccgca cgcgcgtccc
    12421 gctggtcgtc ctcatcgggg cgatcatcgg ggcggtggcc ggctacggcc tccagtactg
    12481 ggcctcgacc atcgcgtacc cgctgaacgt cggggggcgg ccccttcaca gctggcccgc
    12541 cttcatcgtg ccgaccttcg agacgaccgt gctgttcgcg gcgctggcgg cggtattcgg
    12601 catgctcgcc ctgaaccggc tgccccagcc gtaccacccg atcttcaata ccccccggtt
    12661 cgcgctggca tcgcgcgacc ggttcttcct cgcgatcgag gcgcgcgatc cgaagttcga
    12721 cctggttgcg acccgccggt tcctggagag cttcaacccc cgcgaggtga ccgaagttga
    12781 ggaatgacac tgccctgggg gcgctcgtgg tcttctgcgc gctcgcggcc ggcggctgca
    12841 ggctcgacat gcacgaccag cccaagtaca cgccgctgcg gcagagcgac ttcttcgccg
    12901 acggacgcgc ggcgcggccg ctggtcgagg ggaccgtcgc gcggggcctg ctcaacgagg
    12961 acgagctgct gatcagcggg aagacggacg gcaagctggc cacggagttc ccgttccccg
    13021 tcaccgccga ggtcgtcgct cgcggccgcg agcggtacga cgtcttctgc tcgccgtgcc
    13081 acgaccggac gggcgccggg aacggcatga tcgtgcgccg ggggtaccgc gcgccgccct
    13141 cgttccacca ggaccgcctg cggcaggcgc cgccggggca cttcgttgac gtgatgacca
    13201 acgggttcgg cgcgatgccg gactaccgct cgcaggttcc cccgcgcgac cgctgggcga
    13261 tcaccgccta catccgggcg ctgcagctca gcgccaacgc caccttgtcg gaggtcccgc
    13321 ccgcggagcg cgagaagctg gcgccgggca cgggcccctc gggggagccc cgatgaacgc
    13381 ccacgactcg gttcccgaac tcgccggact ccgccgggcg gccctggcag ccgcggccgc
    13441 cggcgcgctg gtcaccgtcg ccggggcgtt cgtcgacccg acgcagttct tccgctcgta
    13501 cctgatggcg tgggtctact gggcgggcgt cgccctcggc tgcctcggcc tgctgatggt
    13561 ctaccacctg accggcggcg gctggggcgt ggcgatcagg cggctgctcg aggcggcgat
    13621 cgggacgctg ccggccgcgg ccgttctctt cgtgccgctg ctgttcgggc tcggcgccat
    13681 ctacgtctgg accgaccggg ccgccgtgct ggcagaccac gtcctcagcc agaaggccgc
    13741 ctacctcaac gtcccgttct tcatcgcgcg caccgcgatc tacttcctcg tgtggctggc
    13801 gctcgcgcac ttcctcgacc gctggtcgcg acgccaggag gagaccgcgg acccgcggcc
    13861 gggcgagcgc atgaaacggc tgagcggagg cggcctggtg atctacgggc tcgccatgac
    13921 cttcgccgcc ttcgactgga tcatgtcgct cgacccgcac tggtactcga ccatcttcgg
    13981 gatgatcttc atcgccgact cggccctggg ggcgctggcc ttcgttttgg tgatcagcta
    14041 cctgctcgcc cggggcgggg cgttcgggcg cgtgctggcc ccgtcgatcc tcaacgacct
    14101 cgggaacctg ctgctcgcct tcgtgatggt gtacgcctac gtctccttct cgcagctgct
    14161 gatcatctgg tcggcgaacc tgcccgagga gattacctgg tacctgcgcc gcatcgaggg
    14221 gggctggcac ctgctcgcga tcgcgctcgc ggtcttctac ttcgccgtcc ccttcatggt
    14281 gctgctgtcg cgcggcaaca agcggcagcc gcagcggctg gcgctgatcg ccgggggcgt
    14341 gctggcggcg cggctgctga gcgtgttctt cctgatcgcc ccggcgttcc acgagcacgg
    14401 cctggcggcc cactggctcg acgccgcagc gctcgtgggg ctcggtggaa cctggctggc
    14461 gctcttcgtc tggcgcctgt cgtcgcgccc gctgctggcg cacggcgacc ccgaactggc
    14521 gccggcgctc gcacgcaggc actgatcagg aatcggggat cagggacggg gatcagggat
    14581 cggggatcgg gaatcaggga tcgggagtcg ggggatcggg attcaggaac cgggaagcga
    14641 gagcatggcg cacggtcaca cccacgacga gtacgttccg ccgccgggga tgaagtacga
    14701 gacgcgcgac ctgtccacac gcgtggtggt catcttcggc gtgtcgcttc tggtcgcggc
    14761 catcgtcatc cacatcgcgg tctggtggct ctacgcttgg atgggtcgcg ccaacgagtc
    14821 ggcctacgcg cgccagttcc cgctggcccc cgtcggggcg ccgcagctcc ctcccgcgcc
    14881 gcggctgcag acgcagcccc gcgaggagtt gaaacagatg cgccgcgacg aggacgggct
    14941 gctctcgggc tacggctggg ccgacccgaa cgccgggcgc gtgcgcctgc caatcgaccg
    15001 cgcgatgcag ctggtcgtcg agcaggggct gccgtcccgc gccgccggac cggccgcgcc
    15061 gtccggcctt ccgcaggact cgagttccgg gcgcacctac gcgcccgcgg gaaagtagga
    15121 gatgagacat caccttgccg cggccgccgt ggccctgtct ctcgccgccg tcccggcctt
    15181 cgcccagtac aacggcgtgc cgaggcccgg aatgatgacg aagggcgaca agccggcggg
    15241 catcaccccg aacgagctga agaaggtcga gttcgcgcag cggctcgacg tccaggtgcc
    15301 cctcgacctg ccgttcaccg acgaggcggg ccgggcggtc acgctggccc agtacttcga
    15361 cgggcggccg gtgatcctgg ccctcgtcta ctacgagtgc ccgatgctct gcgtccaggc
    15421 gctcaacggg ctcgtgaaga gcctcaagac gttgtcgctc gagccgggac gcgattacga
    15481 ggtcgtcacg ctgagcttcg acccgcgtga gacgcccgcc caggcgctcg aaaagaagga
    15541 acactacgtg gagaggctcg gccgcgaggg aggcgccgcc gcgtggcact tcctgaccgg
    15601 ggacgacgcg gcaatccgcc tgctcaccga cgcggtcggc ttccacttcg tctacgacga
    15661 ggggaccggc cagtacgccc acccgacggg catcgtcgtc ctgacccccg agggccgcac
    15721 gtccaagtac atctacggcc tcgactacgg gccgcgcgac ctccgcctcg ccctggtcga
    15781 ggcggcgggc ggaaagatcg gcacccccgt cgactccctg ctcctctact gcttccacta
    15841 cgacccggag cagggcaagt acggcctcgt gctgatgagc gtgctgcgtc ttgcgggcgt
    15901 cgtgacggtg acctgcatcg gcgcgttcat cctgctcatg tggcggcgcg agaagcgagc
    15961 caggaagtag cccatgaact ggtccctccc gctgtttccc accggcgcct cgaccatggc
    16021 ggggaaggtg gatctgctct acttcgtcct gattgccgtc agcggcctgg ccgtcgccgt
    16081 catctcgctc ctcatcatcg cgttcggcgt ccggtaccgg cggcgcgcgc ccgatcaggt
    16141 cggtgcgccg gtcatcccct cgctggcgct cgagattggc tggaccgcgg tcccgctcgt
    16201 ggtcggcctc gtgctgttcg cctggggcgc caacgtctac ttcgcgcagg cccgtccgcc
    16261 cgtcgagacg ctcgacgtct acgtcgtggg caagcagtgg atgtggaagt tccagcaccc
    16321 gggcggccag cgcgagatca acgagctgca cgtcccggtc gggcgcgcgg tgcgcctcac
    16381 cgccgcctcc gaggacgtca tccactcctt cttcgtcccc gccttccggg tgaaggccga
    16441 cgtgatcccc ggccgctaca ccacgctgtg gttcaacgcc accaagccgg gggagtacca
    16501 cctcttctgc gccgagtact gcggcacgca gcactccggg atgatcggga aggtcgtggt
    16561 gatggagccg aatgcgtacc aggcctggct gggggggggc gcagccgaag ggtcgatggc
    16621 gtcgcagggg cagaagatct tccaggacct cgcctgccac acctgccacc tcgactcggg
    16681 ccagggccgc ggtccgacgc tcgtcgggct gtacggcagc caggtcgggc tggcgagcgg
    16741 ccaggtggtg acggccgacg acgcctacat ccgcgagtcg gtgctatcac ctgctgccaa
    16801 ggtcgtggcg gggttccagc ccgtaatgcc gaccttccag ggcctcgtca gcgaggagca
    16861 gctgctggcc ctgatcgagt acgtgaagtc cctgcaggcg aagccgcagg gaacaccggc
    16921 gacccaggcg cccgccgggc agcagccagc cgccgcgacg cccgccgcgc cgcagccgcg
    16981 ggcgcccggg ccggccggcc ggaaccccgg cggggacagg aactagacgc atggacagcc
    17041 tcacgcttcc gcgcacgcac tacgccaacg ccgagtacgg cctcaggtcg tggctgctga
    17101 ccacggacca caagcgcatc gcgctgctgt tcctcggcag catctcggcc ttcttcctga
    17161 tcggcggcct cttcgcgatg ctgatccgcc tcgagctgct gacgccgccg ggcgacatgg
    17221 tcggggccga cacctacaac aagctcttca cgatgcacgg catcgtgatg atcttcatgt
    17281 tcctgattcc ctcgatcccg gcggtcttcg ggaacttctt cgtcccgatc atggtggggg
    17341 cgaaggatct cgcgttccca cgcctgaacc tgctcagctg gtacatcttc atgatcggcg
    17401 ggctgttcac gctggccgcc atcgcgctgg gcggcgtgga cacggggtgg accttctaca
    17461 cgccgttcag cacccacgcc tcgcagagca acgtgatggt ggccgccgtc ggcgtcttca
    17521 tcatggggtt ctcgtcgatc ctgaccgggc tgaacttcat cgtcacgatc cacacgatgc
    17581 gggcgccggg cctgacctgg ttccgcctgc cgctgttcat ctgggcgatg tacgcgacca
    17641 gcttgatcaa cgtgctcggc acgccggtgc tggcgatctc gctggtggcg gtggccctgg
    17701 agcgcgcgct gcacatcggg atcttcgacc cggcctacgg gggcgacccg gtgctcttcc
    17761 agcacctgtt ctggttctac tcccacccgg ctgtctacat catggtgctg ccgtcgatgg
    17821 gggtggtcag cgagctggtc gccaccttct cgcgcaagaa catcttcggc tacaccttcg
    17881 tcgccttctc gagcctcgcc atcgccgtct tcgggttcct cgtctggggc caccacatgt
    17941 tcgtggcggg gatgtcggtg tacggcgcga tgatcttctc gatcctgagc ttcatggtcg
    18001 ccgttccgtc ggcggtcaag gtgttcaact ggaccgcaac gctgtacaag ggatcgatct
    18061 cgtacgagac gccgatgctc tacgcgctgg ggttcatcgg cctcttcgcg gtcggcggcc
    18121 tcacggggct gttcctcgcg accctggccg tggacgtcca cgtccacgac acctacttcg
    18181 tggtggcgca cttccactac atcatggtcg gcggcgcgct gatgggctac ctcggcgcgc
    18241 tccactactg gtggccgaag atgacgggcc ggatgtaccc cgacctcatt ggacggatcg
    18301 cggccgtcac ggtcttcatc ggcttcaacc tgacgttctt cccgcagttc gtcctcggct
    18361 acctgggcat gccgcgccgc tatcacgcct acgccgagga gttccaggcg ctgaacgtgc
    18421 tgtccaccgc cggggcgtcg atcctggcgg tcgggttcct aataccggcg ttctacctgc
    18481 tctggtcgct caagtacggg aaggtggccg gtgccaaccc gtggggctcg gtcagcctcg
    18541 agtggacgac cccctcgccg ccgccgacct tcaacttcga cgagacgccg gtcatcgccg
    18601 ggccggccta cgactaccgg gaaccggagg cgaagcgtgc ctgacgccgc attcgatccg
    18661 cgggccgtgc ccggggccga cgctcatgcc gccgccctgc acagcccggc gttgaagcat
    18721 cacttcgacg acctcgggca gcagaaggag gcggccacgc ttggcatgtg gctcttcctc
    18781 gccaccgaag tgctgttctt cggcggcctg ttcgccgcgt acctcctcta ccgggtctgg
    18841 tacccggacg tcttcggcga ggccagccgg agcctcgaca tcacgctcgg gtcggtcaac
    18901 acgctcgtcc tcatcggcag cagcctcacg atggccctgg cggtccacgc cgccgccacc
    18961 ggccagcggc gcacgctgat gatcttcatc gccctcacca tgctgctcgg ggcggtcttc
    19021 ctcggcatca agggggtcga gtactacgac aagatcgtgc accaccacgt ccccggcccc
    19081 tcgttccact tcgagggccc aacgcccgag cgcgcgcagc tcttcttctc gctctacttc
    19141 gcgatgaccg ggctccacgc cttccacatg gtgatcggcc tcgggctcat gaccgtgatc
    19201 ctcctgatgg cgtggcgcgg acgcttctcg gccacgtggt acacgccggt cgagatgtcc
    19261 ggcctctact ggcacttcgt ggacatcatc tggatcttcc tgtttccgct gctgtacctg
    19321 gtggacaggc acagctaggg ggccactgcc ctatgtcgca cgtcatcccc cgcaggatct
    19381 actacgtcat cttcgccgtg ctcgtggtgc tgaccctcgt cacgaccgag gtcgcgttct
    19441 tcgacttcgg cctgatgaac ccggtcattg ccctcaccat cgccgtcgtg aaggccacgg
    19501 tggtcgtcct ctacttcatg cacatgaggt acagctcgcg cctgaccatg gtcatcgggg
    19561 cggccgggat cttctggctc gggatcctgc tcgcgctgac gatgagcgac tacctggcga
    19621 ggggggcggg gacgtactga cgcgcgtaat caggctttct tcttcgccag cctggccagc
    19681 acggccagct ccttcagcac ctgcttgatc ggccgcgcgg gcgtgcccca gaacagggtg
    19741 cccgggccgc ggagcacctt gccggtcggc accccgcact gcgcgccgag gatcgctccc
    19801 ccgccaacgg tgacgtggtc ggcgatgcct acctgtcctg caatgatcgc cccgtcctcg
    19861 atcaccgagc tgccggcgat gcccgtctgc gcggcgatga cgacgtcgcg gccaatgcgg
    19921 acgttgtggg cgacgtgcac ctggttgtcc agcttggtgc cgcggccgat cacggtctcg
    19981 cccagcgctc cgcggtccac cgtgcacagc gtgcccagct ccacgtcgtc ctcgatgacg
    20041 aggcgtccga cctgcgggaa cgcctcgtag cggccggtcg ccgcgtcgcg gacgtacccg
    20101 aacccgtcgc tgccgagcac cgtccctgcg tgcacgacca cgccggcgcc gatcctggtc
    20161 cccgggtaga cgaccacgtg cgagcggagc accgtgccgg agccgatgcg aacaccggcc
    20221 ccgacgtggc agccggcgcc cagctggacg aggtcgccca cctcggcccc ctcgtccacc
    20281 acggcattag ggccgacggc aaccccgcgt ccgaggatgg cggcggggtg gacgattgcg
    20341 gtctgatgca cgcccggggg cagcgaggcg ggccgcgaga ggatgcggcc cgcgcgggcg
    20401 aaggccagcc ggggctggtc gcagacgaca agcgcgccgt ggaaggatgc gtccgcggcg
    20461 aaggggccgg cgacgaggac tccggcggcc gatgccagcg cctcgtcgag ggagcgcgcg
    20521 tcctcgacga aggcaagatc ggtgaccctg gccgtcgccg ccgtggcgat gcccgagacg
    20581 ggggtgccgg ggtcgccgat gacgcgcccg ttcaccagcc ctgcgatctc cgcggcgctc
    20641 agggtcaccg ccgggccgcc tcgcccgcgg gcgtcatctc cagccacgag aaccagatcg
    20701 gatgtcgtct cttgtaccat cggaggacct cgaggagggc tttctgcttc tcgggagaga
    20761 tgacctcggg cgggatgcgg tcgaagtgca gccggatcgt cttcgcgtcg aggaactcga
    20821 gcgcctcggc gcacagcgtc tgcagctcgt tgcggatgcg ccccgagtct gagatcttca
    20881 ccggccgcgt ctcgaacggc tcgccggcga ggaagcggtt gagcagcggg ctgaagacca
    20941 gccggctctg ctcgaaggtt tcaccgagcc gcagcgtcag ggccacccgc ttgtccccgt
    21001 gcttcgacac catccgcacg gtggctcgat agactcccgg cgagacctct tcgacgccgg
    21061 gagcgccgga gtaggggtcg ggtcccatgt agccggagag cgcgagcacc tcccaccgct
    21121 tctccgggaa gaagtgatcg gcggtgagcg tcggccggtc gcccagcatc cctgcgtcgc
    21181 gggcctcgag gatcatgcgc ctgtagtcgt ccacctcggc gtccccgagc gaatcgctcg
    21241 caagcgcagc cagccgcgag gcgaagtcgg gcgccgcggg gtcgaggcgc atcacgtcct
    21301 gctcctcgtc gtagggcagg tcgaagcgcg aggtgaaggt ggccatcgag gcgtcgaggc
    21361 ccaggatcgg cgcgatgctg gcggcctgca ccgcctcggc agaagccgcg tagccgtcgc
    21421 acaggaagag gtggcgctcg cccgcctcgt ccagccacgt cccgatccgg taggtcggcg
    21481 cgtaggtgcc cgactcggtg aacgcggcca tgccgccggg cagctcccag ccttcctcga
    21541 cgaggtgagc gcccagctcc tgccagctct tccacaaccg gccgatccgc ccctcgcggc
    21601 tcttgccgcc caacgtccac acgtggacgc ggtcgcgccg gatgccgggc caggtggcct
    21661 cgatcgcctc catcacgagc cggcgcggcg tcctgaagtc gagcaggatg gcgcggtcgg
    21721 cggcctgcgc gacgacctcc ttcggcagga cgagcatccc gacgtacccc tcgtacggcc
    21781 gcgagatctg cagcggctga ttgaagaggt gcaggatcgt catcggcccc gtcggcgagc
    21841 ccttcgcgaa gcgcgacgtg ttctcgaggg tgtcgatggc ggcgccccag accgtgatgc
    21901 ccgattccct gatctcgcgg tagaagccgt cccacccgta gccggggtcg ttcaggaggc
    21961 ggacgacgag gcgatcgagg tgacgggcga tctcggggcg cgcgtacacc cgcccaaagc
    22021 cgatctgcgg gttcgagccc atctcggggg tttcgcccgc cttgggcatc agcccctcgc
    22081 ccaggcacac catgatggcg tggttctcgg gaaggtgtcg cgagaggtac cagaggctct
    22141 cgctcatgac gtacgccgag atcgcgtcgg cgtccttctt cacctggttc aacccagccc
    22201 tgtcgagccg ggccccggcg cccgggtggc cgaagacctg cgtgccgatg gcggtcaccg
    22261 cgccggtcat cgccagcagc cgctccatgc ggccgtcgag gaaggagagg ttccgcgtga
    22321 actccagcac cctctcgccg cgcgtccact ccacctcgac gtcctcgagc cagaggtgga
    22381 agcggccgag gatcggccgc gtgtcgaagg accactgcgc gacgacgtcc tgcttctcct
    22441 gctcgctctc gttcatgggg ggggctcctt cggctcgcag cggggtcagc ggctcgcggc
    22501 cagacgggcc cacgagtcgc gcagcggcac cgagcggttg aacaccgggc ggcccggcgt
    22561 cgaatcggga tccacgcaga agtacccctg ccgctcgaac tggtagtgcg agaacgcggg
    22621 cgcagcgtcg agccccggtt cgatcttgca gcccttcagg acggtgagcg agccggggtt
    22681 gaggtacgac ttccagtcgc gcccctcgtc ggcgtcgtgg ggatcgggca cgctgaagag
    22741 gcggtcgtac aggcggacct ccgcgtcgcg cgcgtgggcc gccgaaaccc agtggatcgt
    22801 cgccttcacc cggcgcccgt ccggcgacgc gccgccccgg gtggccgggt cgtagcggca
    22861 gcgcagctcg atcacgttcc cgtcggcgtc cttcacggcc cgctcgcagg tgaggaagta
    22921 ggtgccgcgc agcctcacct cgcgcccggg cgagagccgg aagtacttgg gcgggggcac
    22981 ctcgcggaag tcgtcctgct cgatgtacag ctcgcgcgag aaaggaacct tccgcacgcc
    23041 catggaggcg tcctcggggt tgaccgggat ctcgatttcc tcgacctggc cctcgggata
    23101 gttctcgatg accacgcgca gcgggcgcag cacggccatc gcccgaggcg cccggcggtt
    23161 caggtcgtcg cgcagggcat cctcgagcag cgccacgtcc acgaggttct cgcgcttggc
    23221 cacgccgacc cgcgacgcga actcccggat ggactccggc gtgtacccgc ggcgcctcag
    23281 gccgacgatg gtcggcatgc gcggatcgtc ccaccccgac accacgccgt cctgcaccag
    23341 ctgcagcagc ttgcgcttgc tcgtcaccgt gaaggtgagg ttcaggcggg cgaactcgat
    23401 ctggcgcggg cggctcggca ccggcaggtt ctcgacggtc cagtcgtaga gcgggcggtg
    23461 gtcctcgaac tcgagcgtgc acagcgagtg ggtgatgtgc tcgatggcgt ccgagatcgc
    23521 gtgcgtgtag tcgtacatcg ggtagatgca ccacttgtcg cccgtgcggt ggtgggacgc
    23581 gtggcggatg cggtagatga ccgggtcgcg caggttgagg ttcggcgacg ccatgtcgat
    23641 cttcgcgcga agcacgcgct caccgtcggc gaactcgccg cggcgcatcc gctcgaacag
    23701 ctcgaggttc tcctcgacgc tgcggttgcg ccacgggctc tcgcgaccag gctcggtcag
    23761 cgtcccgcgg tactcgcgga tctcgtcggc cgagaggtcg tccacatagg ccttgccgtc
    23821 gcggatcagg cccagggtgt actcgtagat ccggtcgaag tagtcggagg cgtggtagag
    23881 gcggtcgccc cagtcgaagc ccagccagtg aacggcgtcg atgatcgagt cgacgtactc
    23941 gacgtcctcc ttggcggggt tggtgtcgtc gaagcgcagg ttgcaggtac cgccgaactc
    24001 ctcggccacc ccgaagttga ggcagatcga cttggcatgg ccaatgtgca ggtagccgtt
    24061 cggttcgggc ggaaagcgcg tgcggaccgt gtcgtgtttt ccggactcga ggtcttcgcg
    24121 gatgatcgca cggatgaagt cgacgggggt ctcggcgctg gtcgggctca tatacccgga
    24181 gtatagcaag cgccgccctc ggaggatttg gaccacgaag atcacaaaga cgaagacgcc
    24241 gcgctggtcg gtcttcggcg gtctcgccga tcctcgatcc ttcgtgtcct ttgtgggtta
    24301 agcgatcgtt gtgtcctttg taatggccat gagcgcgtcc agctcggcaa ccagcggccc
    24361 ctcctccgag cccgcccgca gctcggcccg cgcgcgcgtc acccgccgcg gcgggtccac
    24421 cagccagccg cgcacgacca gcggggtgcc gacgcgcacc ggttcgcgga acctcgcctc
    24481 ggcccgggcc gtcaccccgt gcaggcccgc gtagatcacc gcccacgcca ccgcctcgtc
    24541 catcagcgta aagagcagcc cgccgtggat gacctctggc cagccgacgt gctcctcgcg
    24601 ggcgacgtac tcggcgcggc agccgcctcc ggggtgcggc gcgaacgcga cccgcagccc
    24661 gcgggcattc tcctgaccgc acacgtagca gtgcgggaac atccgttctg caacctcgga
    24721 cacggccgcc tccgccaagg ggcttcgctc tgacctttac gcctgcgccg gcatgccgta
    24781 agccgtccac tcctcgcgcg aggggccata gacgcctggc acgggcaggc cggcctgccg
    24841 catgagcacg gtcatctgcc cgcggtggtg ggtctggtgg gcgatcagga cggcaagcgc
    24901 ctggccgcgc gtccacttct caccgtacat ctcgatcgtc ccctgcaggt cggcctcgct
    24961 ccaggccttc tccaccgccg cgggcagagc ggccgacgac ttctcgtagg tctccacgaa
    25021 ctccctcgcc gttgccggta ccggcgcatc gtcgggcggc gcgtcgatgg tcaagccggc
    25081 gtgacgggcc atctcgccaa ccgacgccac catgtgccag gcgatccggc ccagcgttcg
    25141 cgacccggga gccacctcct gggccagcga cgcatcggtc agcgcgcgaa ggagcttgag
    25201 cgtcccctcg cgctcgacgt cccaccccgc cttgaagtcc gccatcttcg tgaacatcgt
    25261 cgtcctccag ttgccgcgat accgcgccgg tcatccacgc cgcggacgat accttgcccg
    25321 ccggcagggg atcaacatcc gccacgccgg cccgccagcg ttcccgccgt ccgctcgagg
    25381 ccgtccagca ggcgcaccag gtcgccggtc tccgtcctgg ggttcatgat cgtggcgcgg
    25441 aggacccggc gcccccccag cacggtcgtc gtgatccacc cgtcgccgcc ttggttgtac
    25501 gtggagcgga gccgcaggtt gaggtcgtcg agccccgggt cgtcctcgcc gcgatctccg
    25561 acgaagcgga agcacaggat gttcgaggcg ggcacgtggg ccgcgcggaa ggcggggcgg
    25621 gagacgacca gatcgtgcag ttgtgagcag gtttcgcaga gccgctcgta cagggcgccg
    25681 aggcccgccg ccccgtatcg ctgaagggcc acccacacct tcagcgcgtc ggcgcgccga
    25741 gagcagctga agctgcgaag cccctggtcc cagttccgcc cctgcctggc gtgaaacagg
    25801 taaggggcgg actggctgaa tgcccgctcg agatcgtcct cgcgccgcgc cagcaccacg
    25861 ctggccgtca gcggcatcag catcatcttg tgcgggtccc aggcgatcgt gctggcccgc
    25921 ccgagacctg cgaggcgttc gcgatgccgc ggggagagca gcgccgacgc gccgtgcgag
    25981 ccgtccacgt ggaaccagag accgcgctcc tcgcaggcgc gcccgatcgc ctcgaggtcg
    26041 tcgaacgcgc cggtggcggt ggagcccgcc gttgccacga cggccatgac gacacgcccc
    26101 tccgctcgca ggcgatcgag gacgcgcgtc agggcgatgg tgtccatgcc ccaatcgctc
    26161 cccgtcggca ccaggacgac gttgtcggtt ccaatgccca gctcgccaac cgcccgcgcc
    26221 acgctgtagt gggcgtgctc tccgcacacg acggccggcg gccggggccc gaccccgcgc
    26281 gcccaggcgt cgggcatcag cgcctgtcgc gcggccagca gcccggcgaa gttcgcctcg
    26341 gttccgcccg aggtgaacgt cccgcccgac tccgcgggca tgcccgccag gccgcacatc
    26401 caccggatca cccgggcctc gatcatcgtg ccgaccggcg acatctccca gacagcgcac
    26461 gactggttca gcgcggccgt caggctttcg gtccacaccg aggccgcgac gggtgccgag
    26521 acctggtgac ccatcgcgcg cggatgcatc aggcgattgc agtcgggaag gatgtcgcgc
    26581 tcgatccggg ccagcacctc ttcgatcggg cggccggaga caggcagtgc ctcgtcgaag
    26641 cgccgttcca gctcggccgg ggtcagagga gtcgaaaccg ggcctgcccc gccccgcgta
    26701 tcgcggaagt agtcggcgac gagccgggcg aaggcgactc cgacggatgg atcggtatcg
    26761 tcctcgagcg cagcgagcac gtcacgagca tcggccatga aggccgatct tacaggaggg
    26821 atgaaggggc gccatcccgg cgccccgaaa cacctcagcc gaacggctca ggcgcgtgcg
    26881 cccttcttcc tccttcgccg cgccaccacg aggccggcta gcccggacga cagcagcagg
    26941 atcgttcccg gctcgggcac gttggcaccg tcgagcgacg ctccctgcag ccccggcggt
    27001 acgaagagcc cgcccgccac ggggatttgc gaggccggaa cgccggtgag gacgatgacg
    27061 gccacgagca gcccgggtgg catcgtcgtc gacgggggat agcccaacag ctgcccgacg
    27121 aacgggatgg ccggcggcga gccgccagtc gacccgcctc ggctgggaac ctgagcgggc
    27181 ggcccgagga ccggccacgt gcccggcacc tggccgttgg acgtgcgctg cgagaagccg
    27241 ggccagttgc cggtgaacat cgacccggtg gtcgcctcga gcccgaagtc gcccgggttt
    27301 acctcggggc ccgtgacggt caggattccc gaaacgatcg ccaccgggct cggcccggac
    27361 acgaacgcga tcgggttggc ggccgcggga gcggcaagcg cgacaatcag cagcagcgcg
    27421 gccgcgaaga tggaccttcg catggttcag cctccagggg acctcgcgcg cgccaccctg
    27481 tgcagcgcgc gacgtacgtg gggtacccgg aagagcaacc aagcaaaggc agggccgcgg
    27541 agggccgcct cagcggccgg ccggcggctt tgcgccgagc gcctcggcct cgcgccgcgt
    27601 ccagtcgcag accgcggtga tttcctcggc agaaagccgc gcctctgggt gcaggagcac
    27661 gtacttcggg tcgggcatcg ccccggtggt gatctcctcg cagatctcct ccagccgtcg
    27721 cgcttggcgg cggttgtcga gaagtgccca ctccgagaag ttcatgtgcg accgtccttc
    27781 ttcgacgtcg cccgccacga gccaggagat cggggcgacc cggctgtacc atggccaccg
    27841 ggtttcgttc gagtggcagt cgcggcacga acggtcgaga acagccacga cagcggcggg
    27901 cggcccggcc gtagccgcca ggctctgctg cgggtcagac ggcgggttgc ttcgatcgac
    27961 cggcacgaac tgggcgatcg ccaggaggac ggccacgacc gggagggccc agcgggccgc
    28021 gcgacgaacg gtcaggggca tgaggtggcc tttcgctgcg ggcgagccgc gctcactaga
    28081 accggtcatt cccggctcga cgtttcccga ggccgacggg gtattctact gcgcatggca
    28141 cacctgacgg ccgctcgcct tctcgtgctc gccgcgctcg ggctcgccat cgccggcctt
    28201 tcggcgagca cccgagaggc ggcccctgac gactcgtctc ccgccccggt ttcgccggtc
    28261 ccgtcggagc ggcagctccg ctggcacgag ctcgagtact acgggttcct gcatttcggt
    28321 cccaacacct tcaccaacaa ggagtggggc tacggcgacg aggccccggc catcttcaac
    28381 cccgccgcgc tcgacgcccg gcagtgggcg cgcgtcgcac gcgacgccgg catgaagggc
    28441 ctcatcatca ccgccaagca ccatgacggg ttctgcctct ggccgagccg cttcaccagg
    28501 cactcggtgg cggccagccc gtggcggcag gggcgcggcg acgtggtcag ggaactgtct
    28561 cgcgcctgcc gcgagttcgg cctggagttc ggcgtctacc tctcgccctg ggatcgtaac
    28621 cacgcagagt acggccgtcc ggcgtacctg gcctactacc gcgaccagct tcgcgagctc
    28681 ctcggcgggt acggccccgt gttcgaggtc tggttcgacg gtgcgaatgg cggcgatggc
    28741 ttctacgggg gggcgaggga gaccaggcgc atcgacaacc gcacctacta cgactggccc
    28801 gtcacctgga acctcgtccg cgcgctgcag ccgggcgcag tcatgttcag tgacgccggc
    28861 cccgacgtgc gatgggtcgg caacgagaaa gggatcgcgt tcgagtcaac ctggtacggc
    28921 ctcgaccgat cggccaccta cccgggcgat ccgcggtacg ccaaggagtt ggcgcgcggc
    28981 aggatcgacg gcgccgacta cgttccgccg gaggtggacg tctcgatccg cccgggttgg
    29041 ttccaccatc ccgagcagga cgagaaggtc aagagcgtgg aggagctgct ggagatctac
    29101 tacagctcga tcgggcgcgg cgccaacctc ctgctcaaca tcccgcccga ccggcgaggc
    29161 ctcattcccg agggggacgt cgcgcggctg atggagttcc gcagggctgt cgaagacgcc
    29221 ttcgcaaagg atctggcgcg ccgggccgtc gccaccgcga gcacgcagcg aggccagggc
    29281 ccgcagttcg gaccgtcgcg cgcgaacgac ggggacccgg ccacctactg ggccaccagc
    29341 gacggcgtca cgacgggaag cgttgaactg tcgtggaagg cgccggtcgc cttcgagcgc
    29401 atcgtcctgc aagaggcgat cgcgctcggc cagcgagtgc aggggtggcg cgtcgaggcc
    29461 gaagtggacg gcgcctggca gacgctcgcc gagggcgagt cgatcggcta caagcggatc
    29521 gcccgcgtcg gacggaccac cgcgagccgg ctgcgcgtgg ccattacggc ggcgcgcgcg
    29581 tgcccgacgc tcgcgacgat tggcgtgtac tagcgcatca ctccatcctc agtgccatcg
    29641 atggcgcctg cttcatggcc cggcgtgccg gcaggtagca ggcgacgagt gcggcgccgc
    29701 cgaggaccga cgaggctgcc agcagggcga tcggctcgat accggtcacc ccgtacaccc
    29761 atgccgccag caccttcgac aacccgagcg cgagcacgac cccgacgagc aggccgccga
    29821 cggtgagcca cagcccgtcc ccgagtacca gcgccagcac gtccaggcgc gacgctccga
    29881 gcgccacccg gatgccgatc tcgcgcgtgc gccgcgacac caggtaggcc ttgacgccgt
    29941 agacgccgag cgtggccagc agcagcgccg ccaggccgaa tgccgtgaag acctgggccg
    30001 aggcgcacgc catccacacg tggacgctgt cgtcgcggtg gtcgcggagc gtcttcagcg
    30061 agaggagcgg cagcga
//
LOCUS       DAOPQX010000006        27965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2193_length_27965_cov_4.671121, whole genome shotgun sequence.
ACCESSION   DAOPQX010000006 DAOPQX010000000
VERSION     DAOPQX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 27965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27965
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2193_length_27965_cov_4.671121"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(445..521)
                     /locus_tag="PKK95_00720"
     tRNA            complement(445..521)
                     /locus_tag="PKK95_00720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(485..487),aa:Arg,seq:ccg)
     gene            791..940
                     /locus_tag="PKK95_00725"
     CDS             791..940
                     /locus_tag="PKK95_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16756.1"
                     /translation="MFIWLPGARPPSTRKSTSRVSAPGIPNSIQRCSSLNGWSVRTRN
                     PSFSV"
     gene            1079..1411
                     /locus_tag="PKK95_00730"
     CDS             1079..1411
                     /locus_tag="PKK95_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOC16757.1"
                     /translation="MQHMGVDEYVLDVLMPDLVGHDRSPGAFLVYLALWTGLYRSAER
                     RIAVSLQQLAQRTGLSKSAVQAGVRVLKRRGLVAVTKDTPTSVPQYELRRHWLGRRSR
                     ALKGYGRG"
     gene            1529..2698
                     /locus_tag="PKK95_00735"
     CDS             1529..2698
                     /locus_tag="PKK95_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16758.1"
                     /translation="MRITQIETVRRMLWLTAGLLVASGSGIPLGSQEEAVETAQDRPT
                     AEDRGSGPRAADGDDLITAGRHVRIEDEVEGDVAAAGAEVSIQAPVSGYVMSAGRNVR
                     LDGRVGNDLWAAGETVDVDGAVANNAMVAGRVVHLHPGATIGNDASVAGNTVMIEGRI
                     ERDLNIGASTARIAGDIGGSVDARAARVSLLPGASVRGDLLVRAQEPPEISPDARVLG
                     QVRYQHVEQGPGSPSWPFVWLLGFGAILTLGLALNLLAPAWPAKVAETLRRRAIASFL
                     SGLAVFVLAPLAAVVLAVTVVGIPLAVVLGALYVTALVLAGVFVSHRVGDWVLRLMNR
                     PQASPWERLAVGALVVSLGVSLPFVGWLVLLAVLITGAGSLVLERSQRREAVPGR"
     gene            complement(2634..3101)
                     /locus_tag="PKK95_00740"
     CDS             complement(2634..3101)
                     /locus_tag="PKK95_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16759.1"
                     /translation="MCGRPSCAVEAAGAGFRSWSRAPHREDPSPDADPIRDWEASSPL
                     DALRRVRDGMMIGPSLGYAEDLVEELGRAPADVVVTSETLLGAMVGAEAAGVPCVAIA
                     PGIYLFPRPGVPPYGPGLQPARSLVGRLRDRVITALVPLPSAGCVRARGCPHP"
     gene            complement(3148..4755)
                     /locus_tag="PKK95_00745"
     CDS             complement(3148..4755)
                     /locus_tag="PKK95_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002191185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HOC16760.1"
                     /translation="MWTQQPNPLGGIFLSALAAAIPIAFLFWALAIKRWKGHAAGPAT
                     AALAVGVAIAVFGMPVPLALASTVQGALYGLFPIGWIVVAAVFLYRVTVESGQFAIIR
                     DSVASLTSDRRLQALLIAFSFGAFLEGCAGFGAPVAISAAMLVGLGFQPLYAAGLCLV
                     ANTAPVAFGSIGIPILVAGQVTGLDVFEISKMVGRQLPFVSVLVPFWLVAIMAGPKGV
                     KEVWPAALVSGGSFALAQWWTSNYLGPLLPDIISSLVSIACLVVFLTFWRPTRTWRFD
                     EEPAATTVPVRHRPGMLIWAWSPFIVLTILVANWGVRPVKALLDTVTVAFPFPLIHNA
                     VVLPGATGPAPAVYTFNWLSATGTATLLAGIVSVAIARMRPGAALALFGRTLASLAAP
                     LLTIASVLGLAYVGNTSGMTTAMGTALASTGALFPLFSPVLGWLGVFVTGSDTSSNAV
                     FGRLQTVSAEHTGMDPVLAIAANSSGGVTGKMISPQSIAVACAATGLVGQEASLFRFT
                     LKHSLLLLAVIALITFLQATVATWMVP"
     gene            4833..5369
                     /locus_tag="PKK95_00750"
     CDS             4833..5369
                     /locus_tag="PKK95_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16761.1"
                     /translation="MKRKWLASAAAFGIVALMGVPVLAQEMKMSAVPQSPEWQKLATL
                     VGHWEGVYEAPDGTKMNGTLEVRMTGDNSAIMHLMNRDAPHEMVTMIHPDGKRLLATH
                     YCAAHNHPRMALVPSKNAGEVAFDFVDGTNIAPGEHYMRKVIFRFVDADHHEEEWTAM
                     GSDGKLNSAVFKYTRVKK"
     gene            complement(5359..6231)
                     /locus_tag="PKK95_00755"
     CDS             complement(5359..6231)
                     /locus_tag="PKK95_00755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC16762.1"
                     /translation="MRPTRMLAAVACALACSVMAAARGQDQHQEAQEPQSRQQAQRPY
                     QPEIGQEGKDVVWVPTSDTMVEKMLDLAEVTPADYVLDLGSGDGRTVIAAARRGARAH
                     GIEYNPEMVELSRGNAEAAGVTSLATFERADLFEADLSKATVITMFLLPSINLELRPK
                     LLAMAPGTRIVSNTFDMEGWKPDRSEVVTKDCANYCTALLWVVPARVEGTWQLPRGRR
                     LRLAQTFQVVSGALVAGGTRRSLVRPRLRGDHLTFTAGGARYDLRVTGDTMRGTVTTG
                     RTKGEPIEATRVTS"
     gene            6385..7221
                     /locus_tag="PKK95_00760"
     CDS             6385..7221
                     /locus_tag="PKK95_00760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC16763.1"
                     /translation="MAGWFRRVADADRTVAPDPASRPKAWLDEEEDRLIPPRNLWVGR
                     GDSISHYYRWVWEYLAYLTLLTGLQREQAVLELGCSHGRTARGLLSYLRSPGRYVGLD
                     VHGPSIEDARARIEARYPNFHFVQADVRNRHYNPEGRTSADCYSFPLPDASFDVVYAA
                     SLFTHLLPCETRRYFLESARVLKPGGHALFSVFLLDCYRGPGTTTSPFYQFEHPLEGC
                     EGVAVRDPDFPDNAVAYRSDVLASCANEAGLDVVRVLPGLWTESPGFAVNEQDLVLLR
                     RR"
     gene            complement(7225..8055)
                     /locus_tag="PKK95_00765"
     CDS             complement(7225..8055)
                     /locus_tag="PKK95_00765"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC16764.1"
                     /translation="MTWSRPARVAALLVVAVLSLGAQAPGKPRQKPYEPEVGQPGKDV
                     VWVPTAQTLVDEMLDLADVTRRDYLLDLGSGDGRTVITAAKRGVRAHGIEYNPEMVEL
                     AKRNADAAGVAGLATFEKADLFESDLSRATVITMFLLPSINMKLRPALLSLKPGTRVV
                     SNTFKMGDWEPDASFQVTRECVNYCTALLWVVPAKVEGTWALPRGRLILTQAFQKVTG
                     SIASWGATTPISAGRLNGEHLTFSVRGSDYFVVVKGDTMEGTIVTGKKKEPFKATRVR
                     "
     gene            complement(8127..8204)
                     /locus_tag="PKK95_00770"
     tRNA            complement(8127..8204)
                     /locus_tag="PKK95_00770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8167..8169),aa:Arg,seq:tcg)
     gene            8378..8554
                     /locus_tag="PKK95_00775"
     CDS             8378..8554
                     /locus_tag="PKK95_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16765.1"
                     /translation="MAHHDRHPADEAELDRDEREIPDEPRGGDEEEFDDTELDDEDLD
                     EGDVSDEDIRAREA"
     gene            8687..9355
                     /locus_tag="PKK95_00780"
     CDS             8687..9355
                     /locus_tag="PKK95_00780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC16766.1"
                     /translation="MKQLQRIAGVLLDVDGTLVDSNDAHARAWERAFREHGYDVPFER
                     IRPLIGMGGDKILPLVANLDPGSIDGELVADRRRAVFFRDHLPVLRAQRGARQLVQLL
                     RERGLRLAVATSAKAEEIDGLLRAARVDDLVDEVVTNSHAGASKPEPDILQLALHRIR
                     CAPAQAFMIGDTPYDIEAAGRSGIVTIALRCGGSSNQDLAGAFAVYNDPEALANDLDR
                     VLAS"
     assembly_gap    9455..9464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9465..11460)
                     /gene="ligD"
                     /locus_tag="PKK95_00785"
     CDS             complement(<9465..11460)
                     /gene="ligD"
                     /locus_tag="PKK95_00785"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HOC16767.1"
                     /translation="MLATLADAPLQDPSLAYEPKYDGIRAIADVANTGGAGSVRIYSR
                     AGNDKTRQFPEIARALAEYGRGLKAGVVLDGEIVALGQDGEPAGFQQLQGRIHLASEA
                     DVRRVASSAPVAFIAFDILRDGGEDLQGLPLTARRARLERVFENTGSPLLRLSRFVPA
                     DGRALYDEARARGWEGLVVKRLDSRYRAGHRSPDWRKLKITLEQEFLVGGWTEPRHTR
                     GHLGALLLGVRAEGGKLEYVGHTGAGFSGAELERVARLLKAIEIRECPFTTRPQANER
                     PHWVEPKLVVQVRFTEWTSDGKLRHPVYLGLRDDVQAASVTREMPVEQASPPVRDTAS
                     SPVRDTASPPVRDTASPPARGTPSPARKAADPDLAPLVKGAAGRQAKALVARLREIEE
                     AGGDGRLELPGGVRLEVGNLGKVFWSQGRLTKGDLMRYYADVAPYLLPVVEDRPLVMK
                     RYPNGVRGKAFYQQRAPEPLPQGVRVETLPDDTDVPSRLVGGSLATLLYMTQLAAISQ
                     DPWFSRVGSHLDADHVAIDLDPMPGTTFDQVLDAARWVHHELEARGIPGFPKTSGADG
                     LHVYIPMPPHTPYETAVLFCQLIATLVAQKHPKETTIVRSVHARGTRIYLDYLQNVRG
                     KTLACAYSARASEFAGVSTPLSWDEVHRGVRREDFTIRTVP"
     gene            complement(11498..11920)
                     /locus_tag="PKK95_00790"
     CDS             complement(11498..11920)
                     /locus_tag="PKK95_00790"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOC16768.1"
                     /translation="MAKRISSGESKPRRTTTTRRRSTKAAAAEAASAAQPEAAAPASE
                     AMPAAGGAELPGSQAVEAGSSGLMTGGREQTAAAGTNGGTLAPQVARSASSPGIWGRE
                     PDREAIARRAFELFLSRGGAHGHDVEDWLAAERELRKR"
     gene            12181..13380
                     /locus_tag="PKK95_00795"
     CDS             12181..13380
                     /locus_tag="PKK95_00795"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC16769.1"
                     /translation="MARARGRKLLGIAAAVVVLGCLGVYGYTVLAKPASAVDPSRVVA
                     VERGDLARSVVATGKIEPIAKVEIKSKANGIIKELRVDVGDTVRAGQVLAELDRDNLA
                     ARLREAQAALAGAQANLEAAEAELAKNRVEAEGPQVAFARRNLERADQLAREKLISTQ
                     ALDDARSALEQAENQQRVARSQLGVSRARVAQAHATVAQAKAAAERAAEELDNATIRS
                     PIDAVVLSRDVEVGSPVSSILNLGSAATLVMVLGDMSTVYVRGRVDEADVGLVKLGQP
                     ARIRVETFKDRPFEGRVTQIAPMGVEKDNVVNFEVRVSIDNASGELRANMTSNAEIVL
                     EEHAGTLVIPEAAVVYDASREASVEVVSDSAPNGRERRPITTGLSNGTKTEVLKGLKE
                     GDRVILQ"
     gene            13387..14691
                     /locus_tag="PKK95_00800"
     CDS             13387..14691
                     /locus_tag="PKK95_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC16770.1"
                     /translation="MELLRQTWANLTANKLRSFLTMFGIIWGVVSIVILSGLGEGFQR
                     GNRTVLEELGKNIVIIRNGRTSLQAGGTRAGRAVRLTIHDVHELRSRSRLLEHVSPEL
                     MRWGAQVKSEFNASSLQMSGVWPSFQYMRTIEVDRGRLLNQADCDQARRVALIGFDSS
                     RQLFADRDPTGRQVMIDGIPYTVVGRLRKKSQDSNYTGEDNQRLFLPYETMRRDFPLP
                     EAYVTADNLSAIIAAPRPEVTAALIERIDREGIRFMGLDSRGPVEDEVRAILAPRHGF
                     LPEDTEAINFWNTALSTAMFGRMIDGMHRFFVAVSVVTLLLGGIGVMNIMLIAVRERT
                     REIGVRKALGATSAAIQRQFFSEGLALTLVSGAIGFGIGLALSALVNLAPMPERFAGL
                     ILTWQTAAFAVAALTAIGVAASVYPARRASRLPPIEALRYEM"
     gene            14726..15982
                     /locus_tag="PKK95_00805"
     CDS             14726..15982
                     /locus_tag="PKK95_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC16771.1"
                     /translation="MLLKEVILQAANAVRDHRFRASLTMLGIAWGIVTVVLLLAYGQG
                     FHRALSHGFRNAFSDGVGVLRGGQTSTQAGGERAGRRISLKVDDVALVAEQGFVKYAS
                     PEYLDSLTVSWGTRQTSAGVRGVAPEYGIMRAETPETGRFLTHEDVERRRRVAFLGAE
                     VVRRLFSNIPPVGETVRIGGVSFEVVGVLANKAQLSSYYYPDKMSVFIPHTTMRQLWP
                     REFLSNIVFQALDPSLGLKAEEQVRAALAARYHFDPRDDRAVTIFGSRQIMQIADGMS
                     NGLRVVLIFIGTLTLMIGGVGVMNIMLVSVQERTREIGVRKALGARRRHILLQFLLEA
                     LAITAAGGLIGVALSFGLVALVGSQPFLAELIDDPSKQTDIHLLLSPGLLLTASAILG
                     VTGLLSGFIPAFRASRLDPIESLRYE"
     gene            16040..16678
                     /gene="msrA"
                     /locus_tag="PKK95_00810"
     CDS             16040..16678
                     /gene="msrA"
                     /locus_tag="PKK95_00810"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOC16772.1"
                     /translation="MARRITMPAREEALPGRAERMKVPARHHVNGAPLTPPFPPGTAR
                     ALFAMGCFWGAERKFWQTPGVVSTAAGYAGGFTTNPTYEEVCSGRTGHAETVLVVYDP
                     ARVSYEELLRVFWENHDPTQGMRQGNDIGTQYRSAIFYLDAVQHAAAEASREAYQRRL
                     SEEGYDQITTEVAPAPEFYYAEDYHQQYLAKNPGGYCGLGGTGVSCPVGLAR"
     gene            complement(16759..16835)
                     /locus_tag="PKK95_00815"
     tRNA            complement(16759..16835)
                     /locus_tag="PKK95_00815"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16799..16801),aa:His,seq:gtg)
     gene            complement(17014..17943)
                     /locus_tag="PKK95_00820"
     CDS             complement(17014..17943)
                     /locus_tag="PKK95_00820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOC16773.1"
                     /translation="MRPDRPLTEPRWPQTRTELDDWIRDHLTISPTDRARLLTAIDVV
                     FTRHQRLWQESKEEAIQALSTGFREKIARLRTELSARDATVSSISRYFEELVDDLTDR
                     SHRDPKTRLMNFDWFMQQLESFLELEQRVRFSAIGLVDIARFKWYNDTLGHGLGDRVL
                     EQVATLLREHARAQDVLAKEVSSSRRPDLHARFGGDEFCFLTPDLAGPAEARVIAERF
                     RRSVEGYDWERLDGRLAQQPVKADVGVVCLRMGPVADRRFISRRLALRLLETADQLMY
                     NAKAEGSIRAHVAAMRVENGELIADEQAARQAS"
     gene            complement(18044..19198)
                     /locus_tag="PKK95_00825"
     CDS             complement(18044..19198)
                     /locus_tag="PKK95_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOC16774.1"
                     /translation="MQDARRAGRRDFIRNSALAGGVLALPAGLQGQGARPPKPRAPRA
                     RMTLKWRPFTAQLRHVFTIAVSSRSTTPDVLTEIEYDGLVGYGEASMPPYLGETQESC
                     IAFLSKVDLSKYPNPFELETILADVDAIAPGNPAAKASIDLALHDLVGKLMGQPWYNI
                     WGFDPVKTPHTSYTIGIDTPDVVRQKVREAKAFKVLKVKLGRDNDHEMIDTIRSVTAV
                     PLTADVNQGWKDRQRALEELHWLASRNVVVVEQPFSKDGPLDDLAWLTERSPIPILAD
                     ESCQRLPDVRRLLGAVSGINIKLMKATGMREAHRMLTLARALGMKVMLGCMTETSCAI
                     SAASHLSPMVDWADLDGALLISNDPFKGTTVVDGKVTLGPGPGLGVTPVS"
     gene            complement(19270..20013)
                     /locus_tag="PKK95_00830"
     CDS             complement(19270..20013)
                     /locus_tag="PKK95_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC16775.1"
                     /translation="MPAPEQPLVLWRLRGHGRDVRCLRHRSGEGFELRVLWGQQLFLT
                     EAFHDEARLLRRAEEFRQGLLSRGWQPLALDPRAPLDMGDPDDVVIGLEPGMDEAAYL
                     AELDRLSPPDLGRRPTVLVVDDEPSVRGFLRSYLEQAGYAVCEAGNIEDALAALDQSP
                     VDAVILDVRMPDPTGLGRSGLEVLTYMRLHASMAAMPVIAVTGHTLDAAEEDILKRNR
                     ADLFMKPDGYRSLLQRLDQLTGRRELTRN"
     gene            complement(20040..20828)
                     /locus_tag="PKK95_00835"
     CDS             complement(20040..20828)
                     /locus_tag="PKK95_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOC16776.1"
                     /translation="MTDAERPRIGCSGWQYRHWRGSFYPTDLAATAWLEHYARTFDTV
                     EVNNTFYRLPEAATFQAWARRVPAGFVFAVKASRYLTHLKRLRDPEEPLARLFDRAGH
                     LGPHLGPVLYQLPPAWPRDLPRLERFLSALPRGHRHAVEFRDPDWYSVETFEALERHG
                     AALCLHDMQGAEPPRRPCGPFVYLRLHGAGDRYGGRYPDRAVDRWADWLAARHHDRLP
                     LFVYFNNDIGGHAPRDAERLRDRLAKLLVGGSGEKQAESGEKQA"
     gene            20916..23192
                     /locus_tag="PKK95_00840"
     CDS             20916..23192
                     /locus_tag="PKK95_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOC16777.1"
                     /translation="MSALLRRDILTARPSRPSIPYIVLFAGLAASAATGFYADRLGAA
                     RDRIRFDSEAEQVRFTVANRIDTYVALLRSASGLFAASEEVTRRDFFRFVERLDLRER
                     YPGMQGIGFSQRIPAAELDHTVARIRASGDPGFRIRPSYPRDEYHAIVYLEPRDRRNA
                     AAVGYDMYSEPTRREAMDAAARKGSAAASGKVTLVQEIEPRERQAGFLIYVPVYRSGF
                     IPATEEARLEQLEGFVYSPFRAGDFLATVLKESGVEGLGLRVYDGPPAFERLLDESGD
                     VPPSGGPPRFERALALDVAGRMWTLVVLTGPSFGANSQSQALQWILTIGGLASGLLFL
                     ATRAEVRARSEAERVALELARSEETLRAQEADLRRLVEAERHAHAEAAAANRAKDEFL
                     ATLSHELRTPLNAILGWATMLKNGQLKSDQAARAVEVIARNAQAQAEMIEDLLDVSRI
                     ITGKLRIQARAMLLAPAIQAALDAVRPAAEAKGVALDWHVDSDGPVLGDADRLQQVAW
                     NLLSNAIKFTPEAGRVSVRLGAEGSTVLLAVRDSGIGIDPAFLPHVFDRFTQHDSSTT
                     RSHGGMGLGLAIVRHLVELHGGSVTAESEGEGQGATFTVRLPLRTAENGEERAAASGD
                     ECELVAGAELAGVRALVVDDERDSRDLVASVLAQSGASVFVAASAREALDVLGRQHVD
                     VVLADIAMPDADGYWLIEHIRSHPSAVIRGLPALAVTAYVRQEDEQRARLAGFQEHVR
                     KPIDVDALRGAVRRLVIA"
     gene            23301..23903
                     /locus_tag="PKK95_00845"
     CDS             23301..23903
                     /locus_tag="PKK95_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOC16778.1"
                     /translation="MGRIGFSSAVLAAFFLAAPAMAQTTAQTPAKEPATAPLEVERWT
                     VTPFIGAGFSGDLENSPLNLGAALGYNVTSRVTIEGQFAYMGAEQGDLVQFDTNVYTL
                     DANVLYHFTEQFAERRWTPYVALGLGMIRANADIDTTDPIFQALDDSTTKLAVNFGGG
                     VKTGISDNVQVRGDLRYYTGDDLAPSFWRATVGVGFMLGR"
     gene            complement(23921..24931)
                     /gene="meaB"
                     /locus_tag="PKK95_00850"
     CDS             complement(23921..24931)
                     /gene="meaB"
                     /locus_tag="PKK95_00850"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOC16779.1"
                     /translation="MKRLPRRRLTADQYVDGILAGDRIVLARAITLVESDLPSDADLA
                     SEVIERCLPHTGRARRIGITGVPGAGKSTFIEALGIHLADERGERLAVLTIDPSSPVS
                     GGSILGDKTRMEKLATHERAFVRPSASRGHLGGVARRTREAMLLCEAAGYPHVFVETV
                     GVGQSETTVRSMVDFFLLLMLPGAGDELQGMKRGILEMTDLIAINKADGENRRRAARA
                     RHEYESALHLFPAAADGWNPRVVTCSALTREGVREIWDAVLEHRTLMESNGWFERHRS
                     EQALEWMKEALLFGLEQEIRRDPAVRRRLDAVQQDVLHGRVSPFRAARELLTLFRQPP
                     GS"
     assembly_gap    25039..25138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25297..>27965)
                     /gene="scpA"
                     /locus_tag="PKK95_00855"
     CDS             complement(25297..>27965)
                     /gene="scpA"
                     /locus_tag="PKK95_00855"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOC16780.1"
                     /translation="RRRVLVGTNSYPNLRERALESADEMPDGWRLASAFEAIRLRTER
                     HAAATGRTPRVLLLERGDLKMRKVRAAFSQNFFGCAGFEIVHGEALSPADLVVLCSSD
                     PEYPALAAEICPQAKVPVIVAGNPKDQADALKAAGVADFVHIQSNAVEVLSRWQDRLG
                     LASAGDARPPLDPARGQKGPAPTAARPSPASRRAAATAPGAEPWLTPEHIEVKPAYGP
                     ADLEGLQHLEYAAGIPPYLRGPYSTMYVMQPWTIRQYAGFSTAEESNAFYRRNLAAGQ
                     KGLSVAFDLATHRGYDSDHERVAGDVGKAGVAIDSILDMKILFDQIPLDQMSVSMTMN
                     GAVLPIMAFYIVAAEEQGVPPEKLAGTIQNDILKEFMVRNTYIYPPAPSMRIIGDIFR
                     YCSERMPRFNCISISGYHMQEAGATADLELAYTLADGLEYVRTGLAAGLDIDSFAPRL
                     SFFWGIGMNYFMEIAKLRAARVLWAKLVKTFGPKNPKSMALRTHSQTSGWSLAAQDPF
                     NNVTRTAIEALAAAMGHTQSLHTNALDEAIALPSDFSARIARNTQIYLQEETGITRVV
                     DPWGGSYYVEALTGALIERAWALIQEVEELGGMTRAIESGLPKMRIEEAAARRQARID
                     SGKEVIVGVNRYVREHEAPIDVLEVDNVEVRRRQVERLEALRRDRDGAAVREALAALT
                     RCAATGEGNLLDLAVDAARKRATLGEISSALEEVFGRYQAVHRTISGIYSAESESDGE
                     FQEARRAAAAFAAQEGRPLRILVAKMGQDGHDRGAKVIATAFADLGFDVDISPLFQTP
                     REVARHAVENDVHVLGVSTLAGGHKTLIPEVIEELKRYGRDDILVVVGGVIPPQDYDF
                     LRQAGAAGVFGPGTVIPRAARQILETLSAPAE"
BASE COUNT         4209 a         9585 c         9904 g         4157 t
ORIGIN      
        1 gagattctcg aacggcggaa gccgctcggc ccgaacgcct acgtgttcgg atccgagtgc
       61 ggcgagtacc agccgaacat tcagaccgga tgggacgcgc tgcggctcct cgcctatggc
      121 atcgagccgc gccggagccg cgggcgggag aacgccgagt ggaaccggtt gcagctgcgg
      181 aagatcgatc tgcactggca cgaccttcgt cacgagggag cctcaagact cctcgccgac
      241 ggcgtggaca tccgcatcat ccagctgatg ctcggacacg ccagcctgca gcagacgcag
      301 cggtacctga atgtgacgga tgaggaattg cgcagagggc tggaggtgag ctggaagcgc
      361 gcccgggtgg tgcagcagga gcctgccaag gctcccgaaa ccccgcacga ttgtcacccg
      421 tttgtcactc agcgggtcga aatgtggcgc gcccgacagg attcgaacct gtggccttcg
      481 gctccggagg ccgacgctct atccagctga gctacgggcg cgcacgggaa gacgcggctc
      541 ggcgggcacc cgcccgctct gccgcagcac ttgagtgtat cacgagcgcc gcccgggctt
      601 cgatttgaaa cacgccgcac gcgccacgaa cgaatcagct cctacctggt gccgagaacg
      661 aacgagttgc catccgggtc cttgaaggtg cagaaggtgc ccagggctcc cgctttggat
      721 cctgtgagaa cgcgacgcct ttcgcctcag ctctcgcgcc gtcccctcga cgtcgtcggt
      781 ccacagcgcc atgttcatct ggctgccggg cgcgaggccg ccgtccacgc ggaagagcac
      841 gagccgcgtg tcggcgccag ggatccccaa ctcgatccag cgctgctcgt cgctgaacgg
      901 ctggtcggtg agaacgcgga atcccagctt ctcggtgtag aaggcaagcg ccctgttcct
      961 ggtcggtcac cgggatgctc acgaacttga cgcctcgaat catcatggtt cctccgtcga
     1021 tcattctcgg gacgaccgcg acgccgcgca agggagaatg gtgctacact atagtgcgat
     1081 gcaacacatg ggagttgacg agtacgtgct cgacgtgttg atgcccgatc tcgtcgggca
     1141 cgaccggtcg ccgggcgcat tcctggtgta cctggcgctg tggaccggtc tgtaccgcag
     1201 cgcggaacgg cggattgccg tcagcctgca gcaactcgcc cagcgcaccg ggctctcgaa
     1261 gtcggcggtg caggcgggcg ttcgcgtgct gaagcggcgt ggcctcgtgg cggtcaccaa
     1321 ggacaccccc acgtcggtgc cgcagtacga gctgaggcgc cactggctcg ggcgccgctc
     1381 gagggcgctg aaagggtacg gcaggggctg aaacctcgac acacctccgt ggcgcaggtc
     1441 ttgaacgtag ctcccatgcg ccggcggtcc ctgccagttc ctgccttgag cttcagccga
     1501 ccgccgagat gcgtaacggg agtactgcat gagaatcacg cagatcgaga cggtccggcg
     1561 aatgctctgg ttgaccgcgg ggctgctcgt cgcatcgggc agcggaatcc cgctcgggag
     1621 ccaggaggag gcggtcgaaa cggcgcagga tcggccaacg gccgaggacc ggggctccgg
     1681 gccgcgcgcg gcggatgggg acgatctcat taccgccggc cgacacgtgc gcatcgagga
     1741 cgaggtggag ggcgacgttg cggccgcggg cgcagaagtg tcgatccagg cgccggtgag
     1801 cggctacgtg atgagcgccg gacgaaacgt acggctcgac ggccgcgtcg ggaacgacct
     1861 gtgggctgcc ggggagaccg ttgacgtgga tggcgctgtc gccaacaacg cgatggtcgc
     1921 ggggcgggtc gttcacctgc accctggcgc caccataggc aacgacgcga gcgtggccgg
     1981 caacacggtg atgatcgaag gccggataga acgcgacctc aacatcggcg cgtccacggc
     2041 tcgcatcgcg ggcgacatcg gcggctcggt cgacgcgcgg gcggcgcgag tgtcgctgct
     2101 gcccggcgcc agcgtacgag gagatctgtt ggtgcgcgcg caggaaccgc ccgagatctc
     2161 gccagacgcc agggtcctcg ggcaggttcg gtatcagcac gtcgagcagg gcccgggttc
     2221 gccgtcctgg ccgttcgtgt ggctcctggg cttcggggca atcctgaccc tgggcctcgc
     2281 gttgaacctg ctcgctccgg cgtggccggc gaaggtcgcc gagacgctgc gcaggcgcgc
     2341 gatcgcctcg ttcctgagcg gactggcggt gttcgtgctc gccccgctgg ccgcggtcgt
     2401 ccttgcggtc accgtcgtcg gcatcccgct ggccgtggtg cttggagcgc tgtacgtgac
     2461 ggcgctcgtc ctggcgggcg tgttcgtctc ccatcgcgtg ggcgactggg tgctgcggct
     2521 catgaaccgg ccccaggcct ccccctggga gcgcctggcc gttggcgcgc tggtcgtctc
     2581 gctgggcgtc tcgctgccct ttgtcggctg gctcgtgctg ctcgcggtgc tcatcacggg
     2641 tgcgggcagc ctcgtgctcg aacgcagcca gcggagggaa gcggtaccag ggcggtgatg
     2701 acccggtcgc gcagccggcc cacgaggctg cgagcaggct gcagcccggg gccgtacggc
     2761 gggacaccag ggcgcgggaa caagtagatg cccggggcga tcgccacgca gggcacgccc
     2821 gccgcttcgg ccccgaccat cgccccgagc agcgtttcgc tggtgacgac cacgtcggcc
     2881 ggtgcccgcc ccagctcctc cacgagatcc tcggcatagc cgagggaagg gccgatcatc
     2941 atgccgtccc gcacgcgcct gagggcgtcg agcggagacg aggcctccca gtcgcgaatg
     3001 ggatcggcgt cgggtgaggg gtcctcgcga tgcggcgccc ggctccacga acgaaagccc
     3061 gcgccggccg cctcgaccgc gcacgacggc cggccgcaca aggtcggccg ggcggcctga
     3121 gccttgagcc gtgagccttg agccgttcta cggcaccatc cacgtcgcca cggtcgcctg
     3181 caggaaggtg atcagcgcga tcacagccag cagcagcagc gagtgcttca gcgtgaaacg
     3241 gaagagcgac gcctcctggc cgaccaggcc cgtcgccgcg cacgccaccg cgatcgattg
     3301 cggcgagatc atcttcccgg tgaccccgcc cgacgagttc gccgcgatcg ccagcaccgg
     3361 gtccatgccc gtgtgctcgg ccgacacggt ctgcagccgg ccgaacacgg cgttcgacga
     3421 ggtgtccgag ccggtcacga acacgccgag ccaccccagc acgggcgaga acagcgggaa
     3481 cagcgccccg gtgctcgcca gcgcggtccc catggcggtc gtcatccccg acgtgttgcc
     3541 gacgtaggcg aggccgagga ccgacgcgat cgtcagcagg ggagccgcca gtgaggcgag
     3601 cgtgcggccg aacagcgcga gagccgcccc gggacgcatc cgcgcgatcg ccaccgagac
     3661 gatgccggcc agcagcgtgg ccgtcccggt ggccgacagc cagttgaacg tgtagaccgc
     3721 cggcgccggg ccggtcgccc ccggcagcac gaccgcgttg tggatcagcg ggaacgggaa
     3781 ggccacagtc accgtgtcga gcagcgcctt cacgggccgc acgccccagt tcgccaccag
     3841 gatcgtgagc acgatgaacg ggctccaggc ccagatcagc atgccgggcc ggtggcggac
     3901 ggggacggtc gtcgccgccg gctcctcgtc gaaccgccag gtccgcgtcg gccgccagaa
     3961 cgtgaggaac acgaccaggc acgcaatcga gacgagcgac gagatgatgt cgggcagcag
     4021 cgggccgagg tagttcgacg tccaccactg cgccagcgcg aagctgccgc ccgagacgag
     4081 cgccgccggc cacacctcct tcacaccctt cggccccgcc atgatggcca cgagccagaa
     4141 ggggaccagc accgacacga acggcagctg ccggccgacc atcttcgaga tctcgaagac
     4201 gtcgaggcct gtcacctgcc cggccacgag gatcgggatg ccgatcgacc cgaacgcgac
     4261 cggtgccgtg ttggccacca ggcacagccc ggccgcgtag agcggctgga agccgaggcc
     4321 cacgagcatc gccgccgaga tggccaccgg cgcgccgaac ccggcgcacc cctcgaggaa
     4381 cgcgccgaag ctgaacgcga tcagcagcgc ctgcaggcgg cgatcagagg tgagcgacgc
     4441 gaccgagtcg cggatgatcg cgaactgccc gctctcgacg gtcacccggt agaggaagac
     4501 ggcggcgacc acgatccagc cgatcgggaa gagcccgtag agcgctccct gcaccgtcga
     4561 ggccagcgcc agcggtaccg gcatcccgaa cacggcgatc gcgacaccga cggcgagggc
     4621 ggcggtggcc ggcccggccg cgtgcccctt ccaccgcttg atcgcgaggg cccagaagag
     4681 gaaggcgatc gggatggccg ccgcgagcgc cgagaggaag atgccgccca gcgggttggg
     4741 ctgctgagtc cacatggcgg gggattatag ccgcggtggg aacctgctcg ggatgcagcc
     4801 cggatagaat ggtgtcttcc aagggaggcc gcatgaagag gaagtggctc gcgtcggcgg
     4861 cggcgttcgg catcgtggcc ctgatgggcg tgcccgtcct cgcccaggag atgaagatgt
     4921 cggccgtccc gcaatctccg gagtggcaga agctggccac gctggtgggc cactgggaag
     4981 gggtctacga ggcgccagac gggacgaaga tgaacggcac gctcgaggtg cgcatgacgg
     5041 gcgacaactc cgcgatcatg cacctgatga accgcgacgc gccgcacgag atggtgacga
     5101 tgatccaccc tgacgggaag cggctgctcg ccacccacta ctgcgccgcg cacaaccatc
     5161 cccggatggc gctggtgccg tcgaagaacg caggcgaggt tgctttcgac ttcgtcgacg
     5221 gcaccaacat cgctcccggg gagcactaca tgagaaaggt gatcttccgc ttcgtggacg
     5281 ccgatcacca cgaggaggag tggaccgcca tggggagcga cggcaagctg aactcggccg
     5341 tcttcaagta cacgcgcgtc aagaagtgac cctcgtcgcc tcgatcggtt cgcccttcgt
     5401 gcggcccgtc gtgacggtgc ccctcatggt gtcgccggtc acgcgcaggt cgtagcgggc
     5461 gccgccggcc gtgaacgtca gatggtcgcc ccgcagccgc gggcggacga gcgatcgcct
     5521 ggtgccgccc gcgacgagcg cacccgagac gacctggaag gtctgcgcca ggcgcaggcg
     5581 ccggccgcgc ggcagctgcc acgtcccctc gacccgggcc ggcacgaccc agagaagcgc
     5641 cgtgcagtag ttcgcgcagt ccttggtcac cacctccgac cggtccggct tccaaccctc
     5701 catgtcgaag gtgttcgaga cgattcgcgt ccccggcgcc atggcgagca gcttcggacg
     5761 cagctcgagg ttgatggacg gcagcaggaa catcgtgatg acggtcgcct tcgagagatc
     5821 ggcttcgaag aggtcggccc gctcgaaggt cgccaggctc gtcacgccgg cggcctccgc
     5881 gttgccccgg gacagctcga ccatctcggg gttgtactcg atgccgtgcg cgcgcgcgcc
     5941 gcgccgggcc gccgcgatca ccgtccggcc gtcgcccgaa ccgaggtcga gcacgtagtc
     6001 cgcaggcgtc acctccgcga ggtcgagcat cttctcgacc atcgtgtctg aggtcggcac
     6061 ccagacgacg tcctttcctt cctggccgat ctcgggctgg tagggccgct gggcctgctg
     6121 tctggattgc ggctcctggg cctcctggtg ctgatcctgg cctctcgccg ccgccatcac
     6181 agaacaggcc agagcgcatg cgacggccgc cagcatccgc gtgggacgca tcctcgacat
     6241 cctacgacct ccgtgcggct tcgcgtgacg ccgcaacagg aagataccac cgcgccgccg
     6301 gacgtgagcg ataatgccgg gcgcgcgccc gcaccgcgcg accgaggcgc cgacgatccg
     6361 cgccttggaa caggaggaga ccggatggca gggtggtttc gcagggtggc ggacgcggac
     6421 aggacggtgg cgcccgatcc ggcgtcgcgt cccaaggcct ggctcgacga ggaagaggac
     6481 cgcctgatcc cgccgcggaa cctctgggtg ggccgcggcg attccatcag ccactattac
     6541 cgctgggtgt gggagtacct ggcgtacctc accctgctca ccgggctgca gcgcgagcag
     6601 gccgtgctcg agctgggatg cagccatggc cggaccgccc gcgggctgct ctcgtacctc
     6661 cgctcgcctg gccggtacgt gggcctcgac gttcacggtc ccagcatcga ggatgcccga
     6721 gcgcgaatcg aggcccgtta cccgaacttc cacttcgtcc aggccgacgt caggaaccgc
     6781 cactacaacc ccgaaggccg gacgagcgcc gactgctatt ccttccccct cccggacgcc
     6841 tcgttcgacg tcgtttatgc ggcttcgctg ttcacgcacc tgctgccgtg cgagacgcgc
     6901 cgctacttcc tcgagagcgc ccgcgtcctg aagccgggcg ggcacgccct gttcagcgtg
     6961 ttcctgctgg attgctaccg ggggccgggt acgacgacct cgcctttcta ccagttcgag
     7021 cacccgctcg aggggtgtga aggcgtggca gtgcgggatc ctgacttccc ggacaatgcg
     7081 gtggcctacc gctcggacgt gctcgcgagc tgcgcgaacg aggcgggact ggacgtggtc
     7141 agggtgctgc cgggactgtg gaccgagagc cccggcttcg cggtcaacga gcaggacctg
     7201 gtgctgctgc ggcggcggtg agggctagcg gaccctcgtc gccttgaagg gctccttctt
     7261 cttgccggtg acgatcgtgc cctccatcgt gtcgcccttc acgaccacga agtagtcgga
     7321 cccgcgaacc gagaacgtca ggtgctcgcc gttcagccgc ccggccgaga tgggcgtcgt
     7381 cgccccccac gacgcgatcg agccggtgac cttctggaac gcctgggtga ggatcagccg
     7441 cccccgcggc agggcccagg ttccctcgac tttcgctggc acgacccaca gcagcgccgt
     7501 gcagtagttg acgcactcgc gcgtcacctg gaaggaggcg tcgggctccc agtcgcccat
     7561 cttgaaggtg ttcgagacga cccgcgtgcc gggcttgagc gagagaagcg ccggccgcag
     7621 tttcatgttg atggagggga gcaggaacat cgtgatgacg gtggcccgcg agaggtccga
     7681 ctcgaagagg tcggccttct cgaaggtggc gaggcccgcg acgccggcgg cgtcggcatt
     7741 ccgcttcgcc agctccacca tctcggggtt gtactcgatg ccgtgcgcac ggacgccccg
     7801 cttcgcggcg gtgatgaccg tccggccgtc ccccgacccc aggtcgagga ggtagtcgcg
     7861 gcgcgtcacg tccgccaggt cgagcatctc gtcgaccagc gtctgcgcgg tcggcaccca
     7921 gacgacgtcc ttgcccggct ggccgacctc gggctcgtag ggcttctgcc gaggcttgcc
     7981 cggggcctgg gcgccgagcg acagcacggc cacgacgagc agggcggcaa cacgcgcagg
     8041 tcgtgaccag gtcatgtgga gatgtgaccc gagggatccc ttcagcccga aagtcaccca
     8101 tgtctccgga atggacctgt ggaggatggc gcgcccggag ggactcgaac ccccgacctg
     8161 cggcttcgaa ggccgacgct ctatccaaac tgagctacgg gcgcgcgccg ctcgatcata
     8221 caccggccgc cgtcacttgc gccggccgag ccgccagacc caccggctga cttcgcgacc
     8281 tcgtggactc caacttcgcg acctcgtgac ttcgcgactt cgcgacttcg cgacttgtgg
     8341 actgatcctt gctgcctctc cgcggggagg acccagcatg gctcatcacg atcgacaccc
     8401 ggccgacgag gcagaactcg accgcgacga gcgcgagatc cccgacgagc cgcgcggcgg
     8461 agacgaagaa gagttcgacg acaccgagct cgacgacgag gacctcgacg agggagacgt
     8521 gagcgacgaa gacatccgcg cccgggaggc ctgatcagcg gtgttcgctt caccgaagag
     8581 gttggcagcg aaggcggcag gaagaaagga cccgggatac tgacacgacg agcgggaggc
     8641 ggggggcggg cgccttcgca gggtgcccgg tccccgctcg acctcgatga agcaactgca
     8701 gcgaatcgcc ggagtgctgc tcgacgtgga cggcacgctc gtggacagca acgacgcgca
     8761 cgcccgggcc tgggagcgcg ccttccgcga acacggctac gatgtgccct tcgaacgcat
     8821 ccgccccctg atcgggatgg ggggcgacaa gatcctgccg ctcgtcgcga acctcgatcc
     8881 cggcagcatt gacggcgagc tcgtcgccga ccggcggcgc gccgtgttct tccgcgacca
     8941 cctgccggtg ctgcgcgcgc agcgcggcgc gcggcagctg gtccagttgc tgcgcgagcg
     9001 gggcttgcgg cttgccgtcg cgacctcggc gaaggccgag gaaatcgacg gcctgctgcg
     9061 ggcggcgcgg gtggacgacc tggtggacga ggtggtcacc aacagccatg ccggcgcgtc
     9121 gaagcccgag cccgacatcc tgcagctggc cctccaccga atccggtgcg cgcccgcgca
     9181 ggctttcatg attggcgaca cgccgtacga catcgaggcc gcagggcgca gcggcatcgt
     9241 gaccatcgcc ctgcgctgcg gcgggtcctc gaaccaggac cttgccggcg cgttcgccgt
     9301 gtacaacgat cccgaggctc tggccaacga cctggaccgg gtgttggcgt cctagggtct
     9361 tccggctttt gccttccgcc ctccacccac gaggcgctcc aggtccaccc ccttcgacgt
     9421 gagcaggccc gcccacaggt ctcccacctc gcgcnnnnnn nnnnccggca ccgtgcggat
     9481 cgtgaagtcc tcgcgcctca cgccccggtg cacctcgtcc cagctgagag gcgtcgagac
     9541 gcccgcgaac tccgacgccc gggcgctgta cgcgcatgcc agcgtcttgc cgcgcacgtt
     9601 ctgcaggtag tcgaggtaga tgcgcgtgcc gcgcgcgtgc acgctgcgga cgatggtggt
     9661 ctccttcggg tgcttctgcg cgaccagcgt ggcgatcagc tggcagaaca ggacggccgt
     9721 ttcgtacggc gtatgcggcg gcatcgggat gtagacgtgg agcccgtcgg cgcccgacgt
     9781 cttcgggaag ccggggatgc cgcgggcctc cagttcgtgg tgcacccacc gggccgcgtc
     9841 gagcacctgg tcgaaggtgg tgccgggcat cgggtcgagg tcgatcgcga catgatcggc
     9901 gtccaggtgc gagccgacgc gcgagaacca cggatcctgc gagatggcgg ccagctgggt
     9961 catgtagagc agcgtcgcga gcgagccccc caccaggcgg cttggcacgt cggtgtcgtc
    10021 ggggagcgtc tccacccgca cgccctgcgg cagcggttcg ggcgcgcgtt gctggtagaa
    10081 cgccttgccc cggaccccgt tggggtaccg cttcatcacc agcgggcggt cttcgaccac
    10141 cggcaggagg tagggggcca cgtcggcgta gtaccgcatc aggtcgccct tggtcaggcg
    10201 cccctgggac cagaagacct tccccaggtt ccccacctcg agcctgacgc ctcccggcaa
    10261 ctccagccgt ccgtcgccgc cggcttcctc gatctcgcgc agccgcgcga ccagcgcctt
    10321 cgcctgccgt cccgcggccc ccttcacgag cggcgccagg tcggggtccg cggcctttcg
    10381 ggcaggcgac ggcgtgccac gggcaggcgg ggacgccgtg tcacgaacag gcggggacgc
    10441 cgtgtcacga acaggcgagg acgccgtgtc acgaacaggc ggggacgcct gttctactgg
    10501 catttcgcgc gtcacgctcg cggcctgcac gtcgtcgcgg aggccgaggt acacggggtg
    10561 gcgcagcttc ccgtccgatg tccactccgt gaagcgcact tgcaccacca gcttcggctc
    10621 gacccagtgc gggcgctcgt tggcctgggg ccgggtggtg aaggggcact cgcggatctc
    10681 gatcgccttc agcaggcgcg ccacccgttc gagctctgcg ccggagaacc cggcgcccgt
    10741 gtgcccgacg tattcgagct tgccgccttc cgcccggacg ccgagcagca gcgcgcccag
    10801 gtggccgcgg gtgtggcgag gttcggtcca gcccccgacg aggaactcct gctcgagggt
    10861 gatcttcagc ttgcgccagt cgggcgaccg gtggcccgcc cggtagcgcg agtcgagcct
    10921 cttgacgacc agcccctccc agccgcgtgc ccgcgcctcg tcgtacagcg cgcgcccgtc
    10981 ggcgggtacg aaccggctca gtcggagcag cggcgatccc gtgttctcga acacccgctc
    11041 cagccgggcg cggcgcgcgg tgagcggaag cccctgcagg tcttcgccgc cgtcgcgcag
    11101 gatatcgaag gcgatgaagg cgaccggcgc gctcgacgcg acgcggcgca cgtcggcttc
    11161 cgaggcgagg tggatgcgcc cctgcagctg ctggaagccg gccggctcgc cgtcctgccc
    11221 gagcgcgacg atctcgccgt cgaggacgac gccggccttc aggccccggc cgtactcggc
    11281 gagcgcgcgc gcgatctccg ggaactggcg ggtcttgtcg ttgcccgcgc gcgagtagat
    11341 tcgcaccgag ccggccccgc cggtattcgc gacgtccgcg atggcgcgaa tcccgtcgta
    11401 cttcggctcg taggcgaggg aaggatcctg gaggggcgcg tccgcgagcg tggcgagcat
    11461 gggccgggcc gtgtgcggaa cgccccccag tctgaggcta ccgcttgcgg agctcgcgct
    11521 cggcggccag ccagtcctcc acgtcgtggc cgtgtgcgcc gccgcgcgag aggaacagtt
    11581 cgaaggcacg ccgggcgatg gcctcgcggt cgggttcgcg gccccagatg ccgggcgagg
    11641 atgccgaccg cgctacctgc ggagccagcg tcccgccgtt cgttccggcg gcggccgtct
    11701 gttcgcgccc gcccgtcatc aacccggacg agccggcctc gaccgcctgg cttcctggca
    11761 actcggcgcc cccagccgcg ggcatcgctt ccgaggcggg tgcggcggct tccggctgag
    11821 cggctgaggc ggcttcggcc gctgccgcct tcgtgctgcg gcgccgggtg gtggtggtgc
    11881 gacggggctt cgattcgccg gaactgatcc tcttggccat gctcgcgaga ctccttgctg
    11941 agtgcctcat taccgcgcac cggaacgcgc cgggcgctgt cgctatggat gtgctgtctt
    12001 gagaatagag actaccttac cacggcaaga cggttcgggt aatccctgcc gcggcaatgc
    12061 aatggtcgag ccagccagag cctccccgaa ggccccgccc ggggaacccc tcacaatgct
    12121 ggtgcgtatt caaccttgac ggcgggtgct acccgcggtg ttgccgcgag ggggagcagc
    12181 atggcacgag cgagagggcg gaagctgttg ggaatcgcgg ctgcggtggt tgtcctcggt
    12241 tgccttggcg tgtacggcta taccgtgctt gccaagccgg cctccgcggt ggatccctcc
    12301 cgcgtggtcg cggtggaacg gggcgacctg gcccgctcgg tcgtggccac cggcaagatc
    12361 gagccgatcg ccaaggtcga gatcaagtcg aaggcgaacg ggatcatcaa ggaactgcgg
    12421 gtcgatgtcg gcgacacggt tcgcgccggg caggtgctgg cggagctcga tcgcgacaac
    12481 ctcgcggcgc ggctgcgcga ggcgcaggcg gccctggccg gtgcgcaagc gaacctcgag
    12541 gccgccgagg ccgagctcgc caagaaccgg gtcgaggccg aggggccgca ggtcgccttt
    12601 gcgcgccgga acctcgagcg ggccgatcag ctggcgcgcg agaagctgat ttccacgcag
    12661 gcgctcgacg acgcccggag cgccctcgag caggccgaga accagcagcg ggtggcccgc
    12721 tcgcagttgg gcgtcagccg cgcgcgcgtc gcccaggcgc acgccacggt ggcacaggcc
    12781 aaggccgccg ccgagcgcgc ggccgaagag ctggacaacg ccaccatccg gtcacccatc
    12841 gacgccgtgg tcctctcgag ggacgtcgag gtgggcagcc ccgtgtcctc catcctcaac
    12901 ctcgggtcgg cggcgaccct cgtgatggtg ctcggcgaca tgtccaccgt ctacgtgcgc
    12961 gggcgggtgg acgaggccga cgtgggcctc gtgaagctcg gacagccggc gcggatccgc
    13021 gtcgagacct tcaaggaccg gccgttcgag gggcgcgtga cccagatcgc gccgatgggc
    13081 gtcgagaagg acaacgtggt gaacttcgag gtccgcgtgt cgatcgacaa cgcgtcgggc
    13141 gagctgcgcg cgaacatgac gtcgaacgcc gagatcgtgc tcgaggagca cgccggcacg
    13201 ctggtgattc cggaggccgc ggtcgtctac gacgcgtcgc gcgaggcgtc ggtggaggtc
    13261 gtttccgatt cggcgccgaa cggccgcgag cggcgcccga tcacgacggg cctgagcaac
    13321 ggcacgaaga ccgaggtcct gaagggattg aaggaaggcg accgggtcat cctgcagtag
    13381 ggtccaatgg aactcctgcg ccagacctgg gcgaatctca ccgccaacaa gctccggagc
    13441 ttcctgacga tgttcgggat catctggggg gtggtgtcga tcgtcatcct ctcgggcctg
    13501 ggcgaggggt tccagcgggg caaccgcacg gtgctcgagg aactgggcaa gaacatcgtg
    13561 atcatcagga acggccgcac cagcctgcag gcgggcggga cgcgggcggg ccgggccgtc
    13621 cggctgacca tccacgacgt gcacgagctg aggtcgcggt cgcggctgct cgagcacgtc
    13681 agccccgagc tgatgcgctg gggggcgcag gtgaagagcg agttcaacgc cagctcgctc
    13741 cagatgagcg gcgtctggcc gtcctttcag tacatgcgca ccatcgaggt cgaccgcggc
    13801 cggctgctga accaggcaga ctgcgaccag gcgcggcgcg tggcgctgat cgggttcgac
    13861 tcgagccggc agctgttcgc cgaccgcgac cccaccggcc gccaggtgat gatcgacggg
    13921 atcccctaca cggtcgtcgg gcgcctgcgc aagaagtcgc aggactcgaa ctacacgggg
    13981 gaggacaacc agcggctgtt cctgccctac gagacgatgc ggcgcgactt cccgctgccc
    14041 gaggcgtacg tcaccgccga caacctctcg gccatcatcg ccgcgccgcg gcccgaggtc
    14101 accgccgcgt tgatcgagcg gatcgaccgc gaggggatca ggttcatggg gctcgactcg
    14161 cgggggccgg tcgaggacga ggtgcgggcc atcctcgcgc cccgccacgg cttcctgccc
    14221 gaggacaccg aggcgatcaa cttctggaac accgcgctct cgacggcgat gttcggccgg
    14281 atgatcgacg ggatgcaccg cttcttcgtc gcggtgagcg tggtcacgct gctgctcggc
    14341 ggcatcggtg tgatgaacat catgctgatc gcggtgcgcg agcgaacgcg ggagatcggc
    14401 gtgcgcaaag cgctcggcgc gacctcggcg gcgattcagc gccagttctt cagcgagggg
    14461 ctggcgctca cgctggtgag cggcgcgatc ggctttggga tcggcttggc gctgagcgca
    14521 ctcgtgaacc tggcgccgat gccggagcgg ttcgccgggc tgatcctgac ctggcagacg
    14581 gcggccttcg cggtggcggc gctcacggca atcggcgtgg cggcgtcggt gtaccccgcg
    14641 cgccgtgcgt cgaggctgcc gccgatcgag gccttgcggt acgagatgta ggggcgaccc
    14701 ttgtggtcgc cctcgaagaa acctcatgct cttgaaggaa gtcatcctcc aggcggccaa
    14761 cgcggtgcgc gaccaccgct tccgtgcgag cctcacgatg ctcggcatcg cgtggggcat
    14821 cgtcacggtg gtgctgctgc tcgcgtacgg gcaggggttt caccgcgcgc tgagccacgg
    14881 ctttcgcaac gccttcagcg acggggtcgg cgtgctgcgc ggcgggcaga ccagcacgca
    14941 ggccggcggc gaacgggcgg ggcggcggat ctcgctgaag gttgacgacg tggcactggt
    15001 ggcggagcag gggttcgtga agtatgccag tcccgagtac ctggattcgc tgaccgtgtc
    15061 ctggggcacg cggcagacgt cggccggggt ccggggcgtc gcgcccgagt acgggatcat
    15121 gcgggccgag acgccggaaa ccggccggtt cctcacccac gaggacgtgg agcgccggcg
    15181 ccgggttgcc ttcctcgggg ccgaggtggt gcgccgcctg ttcagcaaca tcccgccggt
    15241 cggcgagacg gtgcggatcg gcggcgtcag cttcgaggtg gtcggcgtcc tcgcgaacaa
    15301 ggcgcagctg tcgagctact actacccgga caagatgtcg gtgttcatcc cgcacacgac
    15361 gatgcggcag ttgtggccgc gcgagttcct gagcaacatc gtgttccagg cgctcgaccc
    15421 gtcgctcggc ttgaaggccg aggagcaggt gcgggcggcg ctcgcggccc gctaccactt
    15481 cgacccgcgc gacgaccggg ccgtcacaat cttcggcagc cggcagatca tgcagattgc
    15541 cgacgggatg tcgaacggcc tgcgggtggt gctgatcttc atcggcacgt tgacgctgat
    15601 gatcggcggc gtcggggtga tgaacatcat gctggtctcg gtgcaggagc ggacgcggga
    15661 gattggcgtc cgcaaggcgc tcggcgcccg ccggcggcac atcctgctcc agttcctgct
    15721 cgaggcgctg gccatcaccg cggccggcgg gctgatcggc gtcgcgctct cgttcgggct
    15781 ggtggcgctg gtgggctcgc agccgttcct ggccgagctc atcgacgacc cttccaagca
    15841 gaccgacatc cacctgctgc tgtcgccagg gctgctgctg acggcaagcg ctatcctcgg
    15901 cgtgacgggg ctgctgtcgg gcttcatccc cgctttccgt gcatcacgcc tcgacccgat
    15961 cgaaagcctc aggtacgagt agacgccgac tcctcctgct atcatcgggg tgggtacacc
    16021 caaggaggac gatcggaccg tggcgagacg catcaccatg cccgcccgcg aagaagcgct
    16081 gccggggcgc gccgagcgga tgaaggttcc ggccaggcac cacgtgaacg gcgcgccgct
    16141 gacgccgccg ttcccgccgg ggacggcccg ggcgctcttc gcgatgggct gcttctgggg
    16201 cgccgagcgc aagttctggc agacgcccgg agtcgtgtcc accgccgccg ggtacgcggg
    16261 cggcttcacc acgaacccca cctacgagga ggtgtgcagc ggccgcaccg gccacgcgga
    16321 gaccgttctc gtggtgtacg acccggcgcg cgtctcctac gaggaactgc tgagggtgtt
    16381 ctgggagaac cacgacccga cgcaggggat gcggcaggga aacgacatcg gcacgcagta
    16441 ccgctcggcg atcttctacc tcgacgccgt gcagcacgcg gccgccgagg cctcgcgcga
    16501 ggcgtaccag cggcggctgt ccgaggaagg gtacgaccag atcaccaccg aggtcgcgcc
    16561 agcgcccgag ttctactacg ccgaggacta ccaccagcaa tacctggcca agaaccctgg
    16621 cggctactgc ggcctcgggg gaaccggcgt ctcgtgcccg gtggggttgg cgaggtagga
    16681 agcagactca gggctcaagg ctcagggctc aaggcctgtc gcgccgaagt tcgctgaggg
    16741 ttggggggac agggagtgtg gggtggatga cgggatttga acccgcgact tccggagcca
    16801 cagtccggcg ctctaccagc ctgagctaca tccaccgcgc aggcgaaaaa acattgtagc
    16861 aaacggccgg gccggacgac cagtgtccgc cttcgcttcg ctccggctcc cttgcgacct
    16921 cggcccgcct tcgcttcgcc tcgattcact tgcgtccccg gcgaggcttc ggcgcggcag
    16981 gccctgagcc ctgagcctga gccctgagcc ttctcacgac gcctgccggg cggcctgctc
    17041 gtcggcgatg agctcgccgt tctcgacgcg catcgcggcg acgtgcgcgc ggatcgagcc
    17101 ttccgccttc gcgttgtaca tcaactggtc cgccgtctcc agcaggcgca gcgccaggcg
    17161 gcgcgagatg aagcgccggt cggccaccgg ccccatccgc aggcagacca ccccgacgtc
    17221 cgccttgacc ggctgctgtg cgaggcgccc gtcgagccgc tcccagtcgt acccctcgac
    17281 cgagcgccgg aaccgctcgg cgatcacccg cgcctcggcg ggccccgcca ggtcgggcgt
    17341 gaggaagcag aactcgtcgc cgccgaagcg cgcgtgaagg tcggggcggc ggctgctcga
    17401 cacctccttg gccagcacgt cctgcgcccg cgcgtgctcc cgcagcagcg tcgcgacctg
    17461 ctcgaggacg cggtcgccca acccgtgacc gagcgtgtcg ttgtaccact tgaaccgggc
    17521 gatgtcgacc aggccgatcg ccgagaagcg gacgcgctgc tccagctcca ggaacgactc
    17581 cagctgctgc atgaaccagt cgaagttcat cagccgcgtc ttcggatcgc ggtgcgagcg
    17641 atcggtcagg tcgtccacga gctcttcgaa gtagcgcgag atgctgctga ccgtcgcgtc
    17701 ccgggccgag agctcggtgc gcagccgggc gatcttctct cgaaaccccg tcgagagtgc
    17761 ctggatggcc tcctccttcg attcctgcca gaggcgctgg tgacgggtga agacgacgtc
    17821 gatggccgtg aggaggcgtg cccgatcggt cggagagatg gtcaggtgat ccctgatcca
    17881 gtcatccagc tcggtgcggg tctgaggcca cctgggctcg gtgagtgggc ggtcggggcg
    17941 catgggtcca gccctgcgga atgcagggtt cgggccaagg ccggggtagc ggccgcccca
    18001 gggcaaaacc cgccagtccc cgccagaagg cggggccctc ccgctaggaa acgggagtga
    18061 cgcccaggcc ggggccgggc ccgagtgtca ccttcccgtc cacgactgtc gtccccttga
    18121 acgggtcgtt cgagatgagc agcgccccgt cgaggtcggc ccagtccacc atcggcgaga
    18181 ggtgcgaagc ggccgagatc gcgcacgacg tctcggtcat gcacccgagc atgaccttca
    18241 tccccagcgc ccgggccagc gtcagcatgc ggtgcgcctc acgcatgccg gtcgccttca
    18301 tcagcttgat gttgatgccc gacacggccc cgagcagccg gcgcacgtcg ggcagccgct
    18361 ggcacgactc gtcggcgagg atcgggatcg ggctgcgctc ggtcagccac gccaggtcat
    18421 cgagggggcc gtccttggag aacggctgct cgacgacgac gacgttccgc gaggcgagcc
    18481 agtgcagttc ctcgagcgcc cgctgccgat ccttccaccc ctggttgacg tccgccgtca
    18541 gcggcaccgc ggtgaccgac cggatcgtgt cgatcatctc gtggtcgttg tcgcgtccca
    18601 gcttgacctt caggaccttg aacgccttcg cctcgcgcac cttctgccgg accacgtcgg
    18661 gcgtgtcgat gccgatcgtg taggaggtgt ggggcgtctt cacgggatcg aacccccaga
    18721 tgttgtacca gggctggccc atgagcttcc cgacgagatc gtgcagcgcg aggtcgatcg
    18781 acgccttcgc ggcagggttc cccggcgcga tggcatccac gtcggccagg atggtctcca
    18841 gctcgaacgg gttcgggtac ttcgagaggt ccaccttcga gaggaaggcg atgcagcttt
    18901 cctgcgtttc gccgaggtac ggcggcatcg acgcctcgcc gtagcccacc aggccgtcgt
    18961 actcgatctc ggtgagcacg tcgggcgtcg tggagcgcga cgagacggcg atggtgaaca
    19021 cgtggcgcag ctgggcggtg aaggggcgcc acttgagggt cattcgcgcg cgcggcgccc
    19081 tcggcttcgg cggccgcgcc ccctggccct gcagccccgc cggcagcgcc agcacgccgc
    19141 ccgccagcgc ggagttcctg atgaagtcgc gccgtcccgc ccttcgtgcg tcttgcatgg
    19201 tgcctctttc cttgcagggg cctgttcccg gaccgccact gttgggcgtc cccggggcag
    19261 ggggcgcgct caattcctcg tcagctcgcg gcggccggtc agctggtcga gccgctgcag
    19321 cagcgaccgg tagccgtccg gcttcatgaa caggtcggcg cggttccgct tcaggatgtc
    19381 ttcctccgcg gcatccaggg tgtgccccgt gacggcaatc acgggcatcg ccgccatcga
    19441 ggcgtggagg cgcatgtagg tcagcacctc gaggcccgag cgcccgaggc cggtcgggtc
    19501 gggcatgcgg acgtcgagga tgaccgcgtc caccggcgac tgatcgagcg cggcgagtgc
    19561 gtcctcgatg ttgccagctt cgcacacggc gtaccccgcc tgctccaggt aggagcgcag
    19621 gaaccccctc acgctcggct cgtcgtccac gacgagcacc gtggggcgcc ttcccaggtc
    19681 ggggggggac agcctgtcga gctcggccag gtaggcggcc tcgtccatcc ccggctcgag
    19741 cccgatcacc acgtcgtcgg ggtcgcccat gtcgagcggc gcgcgcgggt cgagggcgag
    19801 cggctgccag ccgcgcgaca gcagcccctg ccggaactcc tcggcgcggc ggagcaggcg
    19861 ggcctcgtcg tgaaacgcct cggtcaggaa gagctgctgc ccccagagga cgcgcagctc
    19921 gaagccctcg ccgctgcgat ggcgaaggca ccgcacgtcg cggccatggc cgcgaaggcg
    19981 ccacaacacg aggggttgtt cgggtgccgg catgcgagag cgctcgggac gcgcccacat
    20041 tatgcctgct tttccccgga ctctgcctgc ttttccccgg aaccgccgac gagtagtttc
    20101 gcgaggcggt cgcgcaggcg ttccgcgtcg cgtggcgcgt ggccgccgat gtcgttgttg
    20161 aaatagacga acagcggcag gcggtcgtgg tgccgcgccg ccagccagtc ggcccaccga
    20221 tccacggccc ggtccggata ccggccgccg tatcggtccc cggcgccgtg cagccgcagg
    20281 tagacgaacg ggccgcaggg ccgccggggc ggctcggcgc cctgcatgtc gtgcaggcag
    20341 agggcggcgc cgtggcgctc gagggcctcg aaggtctcga cggagtacca gtcgggatcc
    20401 cggaactcca cggcgtggcg gtggccgcgc ggcagcgccg agaggaagcg ctcgagtcgc
    20461 ggcaggtctc gaggccacgc gggcggcagt tggtagagca cggggccgag gtgggggccg
    20521 aggtgaccgg cgcgatcgaa gagacgggcg agcggttcct ccgggtctcg cagccgcttg
    20581 aggtgtgtca ggtaccggct ggctttgacg gcgaacacga agcccgcggg gacgcggcgc
    20641 gcccaggcct ggaaggtcgc ggcctcgggc agccggtaga acgtgttgtt cacctcgacg
    20701 gtgtcgaacg tgcgcgcgta gtgctcgagc cacgcggtgg cggcaaggtc cgtcggatag
    20761 aagctgccgc gccagtggcg gtactgccag cccgagcagc cgatgcgcgg tctctcggcg
    20821 tcagtcacgc cttcccgcga tgcagccggc ttgccgccgg gcggtagcca cgcgggcgcg
    20881 gacgggctac gatgtgggga aaccgcgatg gcgaaatgag tgccctgctg cgccgcgaca
    20941 tcctgacggc ccgtccgtcc cgtccttcga ttccctacat cgtgctgttc gccgggctgg
    21001 cggcgtcggc ggccaccggc ttctacgccg accggctggg ggccgcacgc gaccggatcc
    21061 ggttcgacag cgaggccgag caggtacgat tcaccgtcgc gaaccggatt gacacctacg
    21121 tggccctcct gcgctcggca tcgggcctct tcgccgcgag cgaggaagtc accaggagag
    21181 acttcttccg cttcgtcgag cggctcgatc tgcgcgagcg ctaccccggc atgcagggca
    21241 tcggcttctc gcagcgaatc ccggcggccg agctcgatca cacggtggcg cggatccggg
    21301 cgtcgggaga tcccggcttt cgcatccggc cgtcctaccc gcgcgacgag taccacgcga
    21361 tcgtctacct cgagccgcgc gaccggcgca acgcggcggc ggtcggctac gacatgtaca
    21421 gcgagccgac gcgccgcgag gcgatggatg ccgccgctcg caagggatcg gcggcggcct
    21481 cggggaaggt gacgctggtg caggagatcg aaccccggga gcggcaggcc gggttcctca
    21541 tctacgtgcc ggtgtacagg tcgggcttca tcccggccac cgaggaggcg cgcctcgagc
    21601 agctcgaggg attcgtctac agcccgtttc gcgccggcga cttcctggcg acggttctca
    21661 aggagtcggg ggtcgagggg ctggggctcc gggtctacga cggcccaccg gccttcgaac
    21721 gcctgctcga cgagtcgggc gatgtgcccc cgtccggcgg gccgccgcgc ttcgagcggg
    21781 cgctggcgct cgacgtcgcg gggcgcatgt ggaccctcgt cgtgctgacc ggcccctcgt
    21841 tcggcgccaa ctcgcagtcg caggcactcc agtggattct cacgatcggc gggctggcca
    21901 gcggcctcct gttcctcgcc acgcgggccg aagtgcgcgc ccgttcggag gccgagcggg
    21961 tggcgctgga gctggcgcgg tcggaggaga cgctgcgcgc ccaggaagcc gacctgcgcc
    22021 ggctggtcga ggcggagcgg cacgcgcacg ccgaggcggc cgcggcgaac cgggcgaagg
    22081 acgagttcct cgcgacgctc tcgcacgagc tgcggacgcc gctgaacgcg atcctcggct
    22141 gggcgaccat gctcaagaac gggcagctga agagcgacca ggcggcgcgc gcggtcgaag
    22201 tgatcgcgcg gaacgcccag gcgcaggcgg agatgatcga ggacctgctg gacgtctcgc
    22261 ggatcatcac ggggaagctg cgcatccagg cgcgcgcgat gctcctcgcg cccgcgatcc
    22321 aggcggcgct cgacgcggtg cggccggcgg cggaggccaa gggggtggcg ctcgactggc
    22381 acgtcgacag cgacggcccg gtgctcgggg atgccgaccg cctgcagcag gtcgcgtgga
    22441 acctgctgtc gaacgccatc aagttcacgc ccgaggcggg gcgcgtgagc gtgcgcctcg
    22501 gcgccgaggg atcgaccgtc ctgcttgccg tcagggatag cgggatcggg atcgatcccg
    22561 cgttcctgcc gcacgtgttc gaccgcttca cgcagcacga cagctcgacg acgcggagcc
    22621 acggcggcat gggcctgggc ctggcgattg tcaggcacct cgtggagttg cacggcgggt
    22681 cggtcaccgc cgagagcgag ggagaagggc aaggggcgac cttcaccgtg aggcttccgc
    22741 ttcgcaccgc cgagaacggc gaggagcggg cggcggcctc cggcgacgag tgcgagctgg
    22801 tggcgggggc cgaactggcc ggcgtgcggg cgctcgtcgt ggacgacgag cgcgactcgc
    22861 gcgacctggt ggcgagcgtg ctcgcccagt cgggggccag cgtgttcgtg gcggcatccg
    22921 cccgcgaggc gctcgacgtg ctcggccggc agcacgtcga cgtcgtgctg gccgacatcg
    22981 cgatgcccga cgccgacggc tactggctga tcgagcacat ccgctcgcac ccctcggcgg
    23041 tgatccgcgg cctgcccgcg ctggccgtga cggcctacgt gcgccaggaa gacgagcagc
    23101 gcgcccgcct cgcgggcttc caggaacacg tgcgcaagcc gatcgacgtg gacgcgctgc
    23161 ggggagcggt gcgccgcctg gtcatcgcct gaagcgccgg ccttcgtcgc tgacgagcgg
    23221 gggcgtgggt acagacgagt tcggccgggc atggcccctg cataggcctc ctgcggaaga
    23281 cgacctcagg aggaacgtgc atgggacgta tcggattcag ctcggcggtg cttgccgcct
    23341 tctttctcgc cgcgccggcc atggcgcaga caaccgccca gacgccggcg aaggaaccgg
    23401 ccaccgcgcc gctcgaggtg gagcgctgga cggtgacgcc gttcatcggc gccggcttct
    23461 cgggcgacct cgagaactcg ccgctcaatc tgggcgctgc gctcgggtac aacgtgacga
    23521 gccgcgtcac gatcgagggg cagttcgcct acatgggcgc agagcagggc gacctggtcc
    23581 agttcgacac caacgtctac acgctggacg cgaacgtgct ctaccacttc accgagcagt
    23641 tcgccgagcg gcgatggacg ccgtacgtcg cgctcggcct cgggatgatc agggcaaacg
    23701 ccgacatcga cacgaccgac ccgatcttcc aggctctcga tgactcgacg accaagctgg
    23761 cggtgaattt cggcggcggg gtcaagaccg gaatcagcga caacgtgcag gtgcggggcg
    23821 atttgcgcta ctacaccggc gacgacctcg cgccgagctt ctggcgtgcc acggtcggcg
    23881 tcgggttcat gcttggacgc tagggctgga agcctagagg ctaggagcca ggcggctgtc
    23941 ggaagagggt cagcagttca cgcgcggcgc ggaacgggct gacccgtccg tgcagcacgt
    24001 cctgctgcac ggcgtccagc cgccggcgga ccgccgggtc gcggcggatc tcctgctcga
    24061 ggccgaacag gagcgcctcc ttcatccatt cgagcgcctg ctcgctgcga tggcgctcga
    24121 accagccgtt cgactccatc agcgtgcggt gttccagcac ggcgtcccag atctcccgca
    24181 ccccctcgcg agtgagggcc gagcaggtga cgaccctggg gttccacccg tcggctgccg
    24241 cggggaacag gtggagcgcg ctctcgtact cgtgccgcgc gcgggcggcg cgacggcggt
    24301 tctcgccgtc ggctttgttg atggcgatga ggtcggtcat ctcgaggatg ccgcgcttca
    24361 tcccctgcag ctcgtcgccg gcgcccggca gcatcaggag caggaagaag tccaccatcg
    24421 agcgcacggt ggtttccgat tgtccaacgc cgacggtctc cacgaacacg tgcgggtagc
    24481 cggcggcctc gcagagcagc atcgcctccc gggtccggcg cgcgacgccg ccgaggtggc
    24541 cgcgcgaggc ggaggggcgc acgaacgccc gttcgtgggt cgccagcttc tccatcctcg
    24601 tcttgtcgcc gaggatgctg ccgcccgaga ccgggctcga cgggtcgatg gtcagaacgg
    24661 ccaggcgctc gccccgctcg tcggcgaggt gaatgccgag cgcctcgatg aaggtgctct
    24721 tgccggcgcc gggcacgccg gtgatgccga ttcggcgcgc gcgcccggtg tgcggcaggc
    24781 accgctcgat cacctcggag gccaggtcgg cgtccgacgg caggtcgctc tcgaccagcg
    24841 taatcgcccg cgccagcacg atccggtcgc cggcgaggat gccgtcgacg tactggtcgg
    24901 ccgtgagcct tcggcgtgga agtctcttca cacctgcacc catcgcggat ctctcacggc
    24961 cttttgcacc acaaagatca caaagaacac aaagaactcg agcgggtgcc acctgctgac
    25021 ggtcttcggc ggccgctcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
    25141 aacacaaaga actcgagcgg gtgccacctg ctgacggtct tcggcggccg ctcgcggccg
    25201 ccgaaggccg gcggtggcct ttccgaggac tgctccacga gagcggggac cttcttcttc
    25261 gtgtcctttg tgtcctttgt ggttgatcct cgtccgtcac tccgcggggg ccgacagggt
    25321 ctcgaggatc tggcgcgcgg cgcgcgggat caccgtgccc gggccgaaga cgccggcggc
    25381 gccggcctgg cgcaggaagt cgtagtcctg cggcggaatg acgcccccga cgaccacgag
    25441 gatgtcgtcg cggccgtacc gcttcagttc ctcgatcacc tcggggatca gcgtcttgtg
    25501 gccgccggcc agcgtggaga cgccgaggac gtgcacgtcg ttctccaccg cgtgccgcgc
    25561 aacctccctg ggcgtctgga acagcgggct gatgtccacg tcgaagccga ggtcggcgaa
    25621 cgcggtcgcg atgaccttcg ccccgcggtc gtgcccatcc tggcccatct tcgccaccag
    25681 gatccgcagc ggccgcccct cctgcgccgc gaaggcggcc gccgcccggc gcgcctcctg
    25741 gaactcgccg tccgactcgc tctcggcgga gtagatgccc gagatcgtcc ggtgcaccgc
    25801 ctggtagcgg ccgaacacct cctcgagcgc cgacgagatc tcgccgaggg tggcccgctt
    25861 gcgcgccgcg tccaccgcca ggtcgaggag gttcccctcg ccggtcgcgg cgcaccgggt
    25921 gagcgccgcg agcgcctccc gcaccgcggc gccatcgcgg tcgcgtcgca gcgcctccag
    25981 ccgctccacc tgccggcgcc gcacctcgac gttgtccacc tcgaggacgt cgatcggcgc
    26041 ctcgtgctcg cgcacgtagc ggttgacgcc gacgatcacc tccttgcccg agtcgatgcg
    26101 cgcctggcgc ctggccgccg cctcctcgat gcgcatcttc ggcaggccgc tctcgatcgc
    26161 ccgcgtcatg ccgcccagtt cctcgacctc ctggatcagc gcccaggccc gctcgatgag
    26221 cgcgccggtc agcgcctcga cgtagtagga cccgccccag ggatccacga cgcgggtgat
    26281 gccggtctcc tcctgcaggt agatctgcgt attgcgcgcg atgcgggccg agaagtcgct
    26341 gggcagcgcg atggcctcgt cgagcgcgtt ggtgtgcagc gactgcgtgt gccccatcgc
    26401 cgccgccagc gcctcgatcg ccgtccgggt gacgttgttg aacgggtcct gggcggccag
    26461 gctccagccc gaggtctgcg agtgcgtccg cagcgccatc gacttcgggt tcttcgggcc
    26521 gaacgtcttc accagcttcg cccacagcac gcgcgccgcg cgcagcttgg cgatctccat
    26581 gaagtagttc atcccgatgc cccagaagaa cgacaggcgc ggggcgaagc tgtcgatgtc
    26641 gaggccggcg gccaggccgg tgcggacgta ctcgaggccg tcggccagcg tgtaggccag
    26701 ctcgaggtcg gcggtcgccc ccgcctcctg catgtggtag ccgctgatcg agatgcagtt
    26761 gaacctcggc atccgctcgg agcagtagcg gaagatgtcg ccgatgatcc gcatcgaggg
    26821 cgccggcggg tagatgtagg tgttgcggac catgaactcc ttgaggatgt cgttctggat
    26881 ggtccccgcc agcttctcgg gcggcacccc ctgctcctcg gccgccacga tatagaaggc
    26941 catgatcggc agcacggccc cgttcatcgt catcgagacc gacatctggt cgagcgggat
    27001 ctggtcgaac aggatcttca tgtcgaggat cgagtcgatc gcgaccccgg ccttgcccac
    27061 gtcgccggcc acgcgctcgt ggtccgagtc gtagccgcgg tgcgtggcga ggtcgaacgc
    27121 caccgagagc cccttctgac ccgccgccag gttgcggcgg tagaaggcgt tcgactcctc
    27181 ggcggtggag aagccggcgt attgccggat cgtccacggc tgcatcacgt acatcgtcga
    27241 gtagggtcca cgcaggtacg gcgggatgcc ggccgcgtac tcgaggtgct gcagcccctc
    27301 gagatcggcg ggcccgtacg ccggcttcac ctcgatgtgc tctggcgtga gccagggttc
    27361 ggcgcccggt gcggtcgccg ccgcccgcct ggaagcggga gaaggacgtg ccgcagtggg
    27421 cgcgggtccc ttctggcccc gagccgggtc gagcggcggc cgcgcatccc cggcagaggc
    27481 cagtcccagc cggtcttgcc agcgcgaaag cacctcgacc gcgttcgact ggatgtgcac
    27541 gaagtcggcg acgccggccg ccttcagcgc gtcggcctgg tccttcgggt tgcccgcgac
    27601 gatgaccggc accttggcct gcgggcagat ctcggccgcg agcgccgggt actcggggtc
    27661 ggagctgcac aggacgacga ggtcggccgg cgacagcgcc tcgccgtgca cgatctcgaa
    27721 gcccgcgcac ccgaagaagt tctgcgagaa ggcggcgcgc accttccgca tcttcaggtc
    27781 gccgcgctcg agcagcagaa cgcgcggcgt cctgccggtg gcggccgcgt ggcgctcggt
    27841 gcggagccgg atggcctcga acgccgaagc caggcgccaa ccgtcgggca tctcgtcggc
    27901 cgactcgagc gcgcgctctc gcaggttggg gtagctgttg gtgcccacga gcacgcggcg
    27961 gcgcg
//
LOCUS       DAOPQX010000007        27712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2217_length_27712_cov_5.075388, whole genome shotgun sequence.
ACCESSION   DAOPQX010000007 DAOPQX010000000
VERSION     DAOPQX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 27712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27712
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2217_length_27712_cov_5.075388"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKK95_00860"
     CDS             complement(<1..644)
                     /locus_tag="PKK95_00860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC16781.1"
                     /translation="MNSTPTPGSRLARRAWLALGAVVALFLAVLIVAGWAGIDKTRKH
                     GHAASADHGSHQDLYTCPMHPEVRSHEPGDCPICGMDLVKVERPSGGGPPATVAAEGL
                     APVHADAEAARLAGVRTVEAASGSVATTVRAVGNVVPHEGRVRHITTKVAGWIEKLHV
                     NATGQMVRAGEPLFELYSPELLATQEEYLRALRTAEELSTSSIPEVRRGAEDLAS"
     gene            complement(659..1969)
                     /locus_tag="PKK95_00865"
     CDS             complement(659..1969)
                     /locus_tag="PKK95_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC16782.1"
                     /translation="MLDRIRLSVPGLPAVLLALTLPASVAAQAVPSAVTAPEAPGVEA
                     LVERALARAPSLAARGERLEAARIALRAADALPDPMVEAEYQAFNFPRYTIGEDPGSM
                     AGMSLRQGLLGGGRRGARRAIATAEASLRSAERQLSATSLATEVRVQYARLYAIDREQ
                     AVLADAAQILRLLESTVTARYAAGASDQASLLRVQLERTRLGQRQADLQAQRAIVRAA
                     LNRLVDEPPDAPIGAVSALPPPALPGAVPEALADEASRLAPEIAVRQREVDAAAQQVA
                     AARAELKPSWSVGAGVYWQGGTDRMVNLNVGIELPFWKKRKQQPLLEAAEGERRAASL
                     ELASTALEVRSQAASLLVEYGNALQQVGRYRDGLLPQSSAALDATRSSYLAGQGDFVS
                     VLDEFRRWIDLRAELAAREADRYAAQARLAALLTPDSQGGNDAL"
     gene            1968..2156
                     /locus_tag="PKK95_00870"
     CDS             1968..2156
                     /locus_tag="PKK95_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16783.1"
                     /translation="MTIIDSWKKGRLSRRAGKGKPGASGLVGKRALLPGNPARTREDA
                     HRLIPMVIDMRQPTGVGA"
     gene            2243..3334
                     /locus_tag="PKK95_00875"
     CDS             2243..3334
                     /locus_tag="PKK95_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC16784.1"
                     /translation="MSDPTPARWVALLAAVTAVSTLHYVTPAASPALHAFYQRLYYVP
                     VILGAYWFGARGGVLVAALCSLAYAPHIHVAWSQNAAYTASQYAELVVFLSLGLAVGL
                     IATRERRLTNRYREAAESLEHANRELRESGEQLRRAERLSALGEIAAGLAHEIRNPLA
                     GMKGALEIVGARAKAGTPEAEFSAIAAREAQRLEGLVQEFLDYARPRPPRRRPGELPQ
                     VLRRVAGLLGPEAERAGVVLEIEADAAPSGPPVDPEQIQQVVVNVVLNAIQASPRGGQ
                     VRVRSRSEARRTIVEVTDQGPGMAPEARARAFEPFYTTKERGSGLGLAISQRIVVAHG
                     GTIRFEEATGGGTRVEIELPDAGTLPAGA"
     gene            3336..4718
                     /locus_tag="PKK95_00880"
     CDS             3336..4718
                     /locus_tag="PKK95_00880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC16785.1"
                     /translation="MTDQKTVLLIDDDESLRRVVEYNLREDGHEVVTAADGRTGLERF
                     QASPVDLVLTDVRMPEMSGLEVLALVKAMQPDLPVIVLTAHGTIDAAVEAMKAGAFDY
                     LTKPFTREQLLASVRKALELGALATENRQLRQIVRERFSFANLIAGSRAMRAVTDTAS
                     RVAASDTTVLLEGESGTGKELLAKAIHVNSARSRGPFVTVNCAAIPEHLLESELFGHR
                     RGAFTGAVADKQGKFEAAARGTIFLDEVGELPLLLQVKILRVLQEREVDKLGETRPVK
                     VDVRVIAATNRDLEKMTADGSFREDLYYRLAVVSIRVPPLRERADDIPLLVDHFLDKH
                     ASRLGRERPAVDKAVYAAFNRYSWPGNIRELENVIERALVLDRDGVLGLDDLPERLQA
                     SGQRIGNLRIELPDEGISLEDVERELLLAALEKHNWNQTRAAAYLNITRSTLLYRMQK
                     FALERPKTEP"
     gene            4812..5321
                     /locus_tag="PKK95_00885"
     CDS             4812..5321
                     /locus_tag="PKK95_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOC16786.1"
                     /translation="MVAVVAFAAALVVGSFFEYVVHRLMHLRWVLGDRHLQHHRKGWG
                     QGVLGEFRDYLYGVPVIGWLGFPHSVEAGIGFAAGCVAYAFFAGYSHQLNHERPELVF
                     WLPRPVHHLHHAHRQWKQNFGIGVDIWDRLFGTYQVVEWKPERRPFDHPLREFGRIWW
                     RKYNGPEGR"
     gene            5458..6501
                     /locus_tag="PKK95_00890"
     CDS             5458..6501
                     /locus_tag="PKK95_00890"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOC16787.1"
                     /translation="MRSVVLVAIMTWLAGVPLWGQGPSGGSSSLSWFKGNTHTHTLNS
                     DGDSTPDEVVRWYRERGYHFLVVTDHNLVTPVDGLNAVFAFEGRFLVIPGEEISDVVP
                     SGAGEDAPGIPVHVNALGTRTAILPAGGATVAEALQRDVAAVEAAGVPVQINHPNFGW
                     AIAQADLPPSSRPLLLEIFNGHPQVNNLGGNGTLPAEELWDVWLSAGRKVFGVASDDT
                     HHLKRPWDRRAASPGRGWVVVRAATLSADTILGALGRGEFYASTGVELADWQVSPEGV
                     SVAIATRPDDLTRFRIEFIGKGGRVLRQTDGSDASYQFTGDEGYVRVRVTDSNGQRAW
                     TQPYFLFSRASAR"
     gene            complement(6468..10031)
                     /locus_tag="PKK95_00895"
     CDS             complement(6468..10031)
                     /locus_tag="PKK95_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16788.1"
                     /translation="MPPHDKSERDKLLRVIRQVRGRWRWRVVLRSLTVLLGAAVITVL
                     AAAWGLERWRFRPEAIVAFRVVTYLTLVGLGWFFLVRPLARRVTDLQVALYLEEHEPS
                     LEALLLSAVDGSLRQPGEGRTDSEALLERLIHSAVERCERIRDGRELERGSMRRSWQG
                     LAAIGAAAAIVFLGGPSYLRHGAMTLLVPVRGVEASSPYRIEVAPGAATLPRGADLAV
                     SARLVGFTSTEGTLFTRAAAGAGFERATMIPATGDGFEATVFRLRDSIEYFVEAAGVR
                     SPVYKVEVADLPQVERLELEYVFPAYTGLSPQKVENGGDIAVLAGTKVRVRVHSTLPA
                     SSGRIVVEGGQPATLANALETALDGEFEVRRNGSYHVELQAPTGEMVAASPHYAIDVL
                     ADQPPSAVIARPQRDLRPTSLEEVFAEARAEDDFGIARLDLVVSVNGGAERSLRLGGG
                     SRSRQLTAGHTFFLEEMNLQPGDVVSYYARATDTDAIAGAKTATSDIYFLHIRPFRKD
                     YRAAESQGGGQAGGQGAQDPSALSDEQRRIVTGTFNVARDRAKVGATKFREDVVFLGL
                     AQGQLRKRVETLSAQIASRLGAGGAGGMEGVNAGLVEAAKEMREAEKRLQARDPKGAL
                     APEQRALRSLQAAEEAFRDVRIAMSQERGGEGGGGSAERQEELAELFEMELDRLKNQY
                     ETFRRSRQQSSNERVDEMLERLRDLARRQEQEAERQRRLSGGAESGGGSAAAERQRQL
                     AAETEEAARRLERLAREERRADLAEAARQMQQAADAMRRAAASGDRAGFAEASAAAER
                     LGRARRGLESDRTDRLTRTIDEARARVQRLQQEQERIEGEVRGLPGTGAGREAQARQL
                     GERKDAQAGEVADLERGLDRAASDFGRERRDAARKLQEAAEGIRDSRLKEKIRYSRGL
                     IQNAPPDQASTFEEQIRADLAALGGRLDEASKAAASGGARDTRAEALERARDLLRGVE
                     SMSERTREQRSADGSRPAPGSRQQAGNGRQGQQADGSQQGADGGRQQGQEASQGTAGG
                     GQGGEAQGGQQGQAGDQQRAEGSSVGVPPEAGGGGGGRPGRMSPEQARQLRREARERG
                     SEAEQLRREMRALGLNPRELDALIRDLRALDQDRLYDDPAALARLQAQLVEGFRRLEF
                     DLRRRLDEGGEVLLSASDDVPPEYRALVEEYYRALAREKRK"
     gene            complement(10143..12281)
                     /locus_tag="PKK95_00900"
     CDS             complement(10143..12281)
                     /locus_tag="PKK95_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC16789.1"
                     /translation="MSLLAPALLAGLLAIAIPVVLHLVQRERKQVVAFPSLMFLRKIP
                     YQSVRRRVIRHWPLLLLRALVLALLAVAFARPFLPGADAAAGGTGDREVAILLDRSAS
                     MAYGDHFQRAREAARSVVEGLRPGDRASLVLFASDVEIAVRQGGERSALLAAIEAARP
                     AALATRYGPAIQAGAGLLESSPMPRREVVLISDFQGTGWDRGQGVKVAPGIALTTMPV
                     GEARVENAAVVDLAFAREAADQSGSERVTVTARVANRGSTRIDNRPIALEADGRQLDA
                     ARVSAEPGTTASVSFSPLTLPANGLVRVVARLTPDRLPFDDTFHAVLASREKLPVLVV
                     ETAAGQTPFLARALAVSSEPRFEARFLAAAQATPAEIERAAVVVLNDTPPPAGAAGRA
                     LAAAVDRGTGLLLALGERSSWSEAAPDLLPGTLGEIVDRREGRGATIGHVERSHPVFE
                     VFGTPRSGDLAAARVFRFRELAAPAGVLARFDDGAVAVAERRVGRGRVLVWTSTLDGY
                     WNDLVLKPVFVPLLHQAIKYLAGHVQRPAWHVAGEAASPSMLAAGAEGGKAASRTRTG
                     QVALSPSGARLPVPGEGSRQAFVPAEQGFYEIRAAAGEGADGERSVLAVNVDPAESNL
                     ARLDPAELVTAVRQGPGVAAASEARQLSPEDRERRQALWWYLLALAVLALGAEALLAG
                     RFSRRRPVPAAAEVAERSAAAAQESQRLAS"
     gene            complement(12278..13189)
                     /locus_tag="PKK95_00905"
     CDS             complement(12278..13189)
                     /locus_tag="PKK95_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOC16790.1"
                     /translation="MEQTQAMAGARFVDPKVLARLNSLELVARTVVDGFINGLHRAPH
                     LGFSLDFAEHRGYEPGDDLRRVDWRLYARTDRFYVKQFEADSNANLVVVLDVSASMRF
                     GSRGLPKIDYGRYLAASLLYFSHQQRDRVGLVTFDRRIVSYVPPSAKHLDFALFALDR
                     AEAGREGELAAPLLTATERLRRRGIVVVVSDLYEEPDRVAAAVRPLRFRGHDVIVFHL
                     LDPAERDFPFDEASSFEDLESGERLPVVPEALRAEYQELVRRHIEALGRLLLDSRVDY
                     ALLDTSVPLDFALFRYLAARERLRRVR"
     gene            complement(13259..14293)
                     /locus_tag="PKK95_00910"
     CDS             complement(13259..14293)
                     /locus_tag="PKK95_00910"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOC16791.1"
                     /translation="MTIANEDVRVLESQDDVSVAERMTATRSLIASELGKVIVGQAEV
                     VDQVLLTLFVGGNSLIVGVPGLAKTLLVRTMAEVLDLKFSRIQFTPDLMPSDITGTDI
                     IQDDPEIGGRRMVFQPGPVFTNILLADEINRTPPKTQAALLEAMQEHRVTVQGRTYPL
                     DEPFHVFATQNPIELEGTYPLPEAQLDRFMFHVVIEHPPEDEELEVLRTTTAGREVRL
                     DRATGAADLVAFQGLVRRVPVSDPVMRYALALVRASRRGNGAPDFVQKWISYGASVRA
                     AQYLVLGAKARAVTHGRYHVTFEDVRALAHPVMRHRVLTNFHAESEGITTDQIIDRIL
                     EAVPVPRSGM"
     gene            complement(14368..15219)
                     /locus_tag="PKK95_00915"
     CDS             complement(14368..15219)
                     /locus_tag="PKK95_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOC16792.1"
                     /translation="MSRTARATCAIVFATAATVAAFAMPQAEPGSPEGSRIRRLLQGL
                     WGEGRGQSAEPEPVPIPHDGRFVIARIRYTVGTGGFEGGFGRRGSREPPWAHDYPRAE
                     RNFMKLLGRITTIRTYDGAEGGAIVDIGSKELFRYPIAYFCEAGFWTQTDEEAANLRA
                     YLQKGGFLIFDDFRGRDLDNFTAEMRRVLPGMRLIELEPASQVFHSFFEIPSLEFRQF
                     YDREPPVFYGIFAGNDPGKRMLAIVNYNNDISEYWEWSDTGWNPIDLTNEAYKFGVNY
                     VVYGMTH"
     gene            complement(15216..16220)
                     /locus_tag="PKK95_00920"
     CDS             complement(15216..16220)
                     /locus_tag="PKK95_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16793.1"
                     /translation="MRRLATMLPLVLLAVSTPAIAQATHLLVIVGLGGDEENRARFHE
                     WASSLVDAARERGGIPPESLVYLGEDTARDPGRIGGRSTREEIANAVGRLASRSRPGD
                     RVLIVLIGHGAAASGPPRFNLPGPDLSPADFARLLDRLGGRTVAFVNTASASGAFLGP
                     LSGRNRVVITATRSDGERNQTRFPGFFVEALASTDADLDKDGRVSMLEAFTWARQRTA
                     ASYEQDGQLLTEHALLDDDGDGKGSAAPGSDGADGALARTVFVSAGGAERAGPPADAD
                     PGLLALYEERGALERRIGELKATRDGMPPAQYQQELESLLLELARKTREIREREKGNR
                     "
     gene            complement(16217..18529)
                     /locus_tag="PKK95_00925"
     CDS             complement(16217..18529)
                     /locus_tag="PKK95_00925"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOC16794.1"
                     /translation="MGAGHGHLPGHGLAGGDVAEDLFALLFKYRPVVFEQGGFAFSPP
                     WPAAAAVLLVLAAAGLTLWAWHRDGRPSGRAVLLLALRGTTLAVLFFALLRPTVTVKA
                     VEARQNFLAVLLDDSQSMSISDRDGVPRSAFVRSAFGHDGSLVRALGDRFALRFFRVS
                     SSTERVPGPEALAFDGTRSHLGEALGRAASELAGLPVSGLVLVSDGADTSASDISPML
                     RSIQAAGLPVFTIGLGREAFDRDVQLGRVEPPAWVLQGSTLIVQVSVAQTGYAGRTVP
                     LLVEDEGRRVGSADVLLPRDGEPVTARVRLTLADAGPRLLRFTIPPLDGEQVTRNNAR
                     EVVVDVRDRREKLLYVEGEPRFEMKFLRQSLAGDEHLQVVTLQRTAERKFLRLDVDGP
                     DDLLGGFPKTRDELFAYRGLVLGSIEAAAFTPDQLRMIADFVNVRGGGLLALGGRHAF
                     AEGGYAGTPVAEALPVRLDAPPLPDDRHETVSARPTRLGQLHAVTQVAATEETSAARW
                     KTLPALTTVNPIRSLKPGAAALLEGEGERVVLAHQRYGAGKALAFPVQDSWLWQMDAS
                     IPVEDQTHETFWRRLARWLVEGVPDRVEVSLDRERVEPGEAVRVAANVRDARFLAVND
                     ASVRARITDPSGRETELPLDFAPDREGEYAASYVPAAAGLHTVRLEAARGRDQLGAAV
                     AYLRAAPGDAEYFDAAQRAPLLKRIAEETGGRYYTPETAASLAEDATYLGRGVTATRE
                     MDLWDMPAVLLLLVGLVGAEWFLRRRSGLA"
     gene            18658..21207
                     /locus_tag="PKK95_00930"
     CDS             18658..21207
                     /locus_tag="PKK95_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC16795.1"
                     /translation="MAAFAVAGVLGLAAWLAVDTAGRREPAHAMAPQRGGGGAAADAP
                     AAFSQEVARAIAQGRRQEAEAMARGRGADDPDAAAALARLVSDRGDYDQAETLLRAAS
                     RHARGGQAALELGLLLQFRGRPDEARAVLEPLASGPEGSDATAALRAGVASRALGLAH
                     EANEWLLDAARLAPGDPAVHVARGELFLERHDRDEAASLFRQALERDARWAPAHLGLA
                     RALADENTPAAEAAARRAEEIDPGMAGAPLLRAEAALRADRLDDGRAALDRALEINPR
                     NPAAHALAAALAHLEGRAADAGASVEAALAVNPTYGDAFRVPAEQLASSGRFEDAVPL
                     ARRAAALEPWNTRALADLGMHLLRVGEEAEARPVLERAFKADPFDLVTFNLLQMLDTL
                     DQFVTVPAGVGRIRLDPAEAPVLQPYAVRIVEEALARYGQKYGLQPRGPVLVEIFPKH
                     DHFAVRTLGIPGLLGALGACFGRVVTQDSPRARPPNTFNWQATLWHELAHVFTLQLSD
                     YRVPRWLSEGISVYEEGRVRPEWAGDSEMTFARVYAAGNLPKLVDFNAAFTRPDLVAA
                     AYFQASLVVGLLVERHGEAVLRTLMSAYAAGASTEEALRKATGAGLSDLQSAFDAMVR
                     ERYGDAARALETPKNVEIPRSAPAAALVEIADRHANSFPVQMAAGRAMAAAGAHAEAI
                     AAFERAARLVPFARGDDSPRAHLARLALAGGDTARAASELEALVRHDHANVDAARQLA
                     SLAEKAGNEPLRLLAYERIVEVDPFDAAAHAALGRIAMGRRDAALAVREFQAALAAGP
                     VDRVAAHCDLAEAYLLGGRSADARREAVAALEIAPTFERAQELLLKTTEGK"
     gene            complement(21224..22180)
                     /locus_tag="PKK95_00935"
     CDS             complement(21224..22180)
                     /locus_tag="PKK95_00935"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOC16796.1"
                     /translation="MTPRVTSALIMAMHILDAVGNTSLVRLRKVVPPGCASVFVKLEW
                     ENPTGSVKDRMARAVISRAEEDGRLRAGWTVLEYTGGSTGASLALVCAAKGYPLRIVS
                     SDAFSREKLDQMRALGATLTLVPSEGGLTTRKLILDMIETARRLSEEPGTYWTDQLNN
                     QDSLAGYHPLAEEIWQQTNGKVDAFVHCVGTAATIRGVATVLRRHKPGILIAAVEPAE
                     SSVLSGGQPGPHKIEGVGIGYEPPLWDPSLVDEVHTVSTGEAKEMTRRLAREEALFGG
                     TSSGADVVAAIRVAERLGQGATVVTLMADSGLKYVNTDVYRD"
     gene            complement(22237..22692)
                     /locus_tag="PKK95_00940"
     CDS             complement(22237..22692)
                     /locus_tag="PKK95_00940"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HOC16797.1"
                     /translation="MPRAAVEAPKALPAGSRGLGETVMAIHVVRLGSRRTPGEGLRVG
                     TVRRPPRGVPKSEHASRDFYDVWLPDLAPSEGLVKEAQQAGDERAWRQFVRHYRAEMK
                     RPEAARLLDLLAALSHRTNLAVGCYCEDEARCHRSVLRELLREHGAELV"
     gene            22797..23423
                     /locus_tag="PKK95_00945"
     CDS             22797..23423
                     /locus_tag="PKK95_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOC16798.1"
                     /translation="MLSEDIFKILLAIGIGGAIGLEREYRDKAAGFRTLIFICVGATL
                     FAMLSSKLAGDRDPTRIAANIVSGVGFLGAGVILRDGGRVVGLTTAATIWLVAALGTG
                     LAGGYYALVLGATALIEVVLWGFPFLEHWIDRIREERNYEVVVGRVEKCQAVEDVFRE
                     CGLRIVRHRESRVGPRLACTWQATGTPQAHRRLIERLLADAEIDEVRA"
     gene            23420..24166
                     /gene="bshB1"
                     /locus_tag="PKK95_00950"
     CDS             23420..24166
                     /gene="bshB1"
                     /locus_tag="PKK95_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HOC16799.1"
                     /translation="MTSLDLLVFGPHPDDLEIGLGGTIALHAAAGWRVGLCDLTRGEM
                     GSNGTPEERLREAEEARVVLGAEWRENLGWPDRGITGDRAQLDQVTRLVRVWRPRVVA
                     IPYWHDRHPDHVAASRVLSEAIFNARLRRYQPEGGGPGGWAPEWVCYYFINDDGPISF
                     AVDVSAHYDAKRRALECYRSQFMPEGPGAVDTRLTAPTFARLISSRDARFGALAGVEY
                     AEGVVVKEPVLRPGLLKDWTAQPAPRSDRR"
     gene            24163..25293
                     /gene="bshA"
                     /locus_tag="PKK95_00955"
     CDS             24163..25293
                     /gene="bshA"
                     /locus_tag="PKK95_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HOC16800.1"
                     /translation="MKIAIVCYASIGGSGIVATELAKCLAVRGHQVHVVSREQPFRLG
                     DDEPGVAFHQVLTPSYPLFREPQYLLSLATRIAQLSREVGLDIIHAHYAIPHATAAYL
                     ARQILESTGGPTPKVVTTLHGTDITIVGSDPSYAETIGFSIDRSDGVTAVSESLKADT
                     YRGLPITRDIRVIPNFLDCRIHHRVPANGLRDRFAGHGRSVVIHVSNFRPVKRIDAVI
                     DVFARIRARRPSVLLLVGEGPDLPLARELAQQRGIEEDVLALGGQEDVRQFLSIADLF
                     LLPSATESFGLAALEAMACDVPVVASRVGGLPEVIEHGVSGYLHPPEEVGAMAESGLA
                     ILTDPGLHARVAAAARRVVHDRFCAEKIVPRYERFYEEVLAG"
     gene            25326..26321
                     /locus_tag="PKK95_00960"
     CDS             25326..26321
                     /locus_tag="PKK95_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC16801.1"
                     /translation="MTSLERLQNVLGARPVDRVPNFDIFMTRAAHHIGAPLSRYYLDH
                     RVLAAANLAVLDDFGLDIVQAISDPYREAADTGLEVTFPDDGLPVSTAPLIVEHSDLA
                     RVRFPVAGFGPRMTDRLEAIRVMRAEVGDEVPVMGWVEGALAEAADLRGMSQVMLDLV
                     MSPGWLEELLERCVEVGVAFAEAQVEAGAHIVGLGDAVASQISPAMYREFALPYERRV
                     FEAIKAKGGIGRLHICGNTTHLLGDMAGSGAAIVDVDWMVDLGRAVDTMRDVVVCGNV
                     DPVAVFLQGTPAQVREAVRANAAAVGGARWVSAAGCEIPDETPAENLLAQREALA"
     gene            26318..26845
                     /locus_tag="PKK95_00965"
     CDS             26318..26845
                     /locus_tag="PKK95_00965"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOC16802.1"
                     /translation="MTLFTIGHSTRPIDEFLGLLRGHGVRRLVDVRTVPRSRHNPQFE
                     AGALAAALEKAGIAYLHLPGLGGLRHPRPDSINTGWRNRSFRGYADHMQSAVFAQSLQ
                     RCIDLAVEGPLALMCAEAVPWRCHRSLIADALLARGIEVREIVSPTSARPHTLTPFAK
                     VSGTTVTYPGAVPKA"
     gene            complement(26963..27697)
                     /locus_tag="PKK95_00970"
     CDS             complement(26963..27697)
                     /locus_tag="PKK95_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOC16803.1"
                     /translation="MAELDRMHALPRRFPGWVGVIGYGLQSAAYGLILQPTVVSLVAA
                     TVFGLFVGLLELLTRRNAAVRQLIPVLSAFVVTYVAFSLLERSGPDIFNTGVLIDRRG
                     RVAGTYRKVQLPQEEVAAGEAPGYAFPVFQADFGRVGLMICHDTSFPEPAVELSRAGA
                     ELILVPIWGGRTPLVRARAIENGVWLATAGYDYDSEIVDPQGNVLASVPHDTAAGAAV
                     AEIDFNRTFPEPWIGNWRDTVGKQRR"
BASE COUNT         3893 a         9987 c         9891 g         3941 t
ORIGIN      
        1 gacgccaggt cctcggcgcc gcgccgcacc tcggggatcg acgaggtgga cagctcctcg
       61 gccgtccgca gggcgcggag gtactcctcc tgggtggcga gcagctcggg cgagtacagc
      121 tcgaagagcg gttcgcccgc cctgaccatc tgcccggtgg cgttcacgtg cagcttctcg
      181 atccagccgg ccaccttcgt cgtgatgtgc cggacgcggc cctcgtgggg gacgacgttt
      241 cccaccgcgc gcaccgtggt cgcgacgctt cccgaggcag cctccaccgt ccggacgcct
      301 gccagccgtg cggcctcggc gtcggcgtga accggggcca ggccttccgc ggccacggtg
      361 gccgggggtc cgccgccgga cggtcgttcc accttcacca ggtccatccc gcagatcggg
      421 cagtcgcccg gctcgtgcga acgcacctcg gggtgcatcg ggcaggtgta gaggtcctgg
      481 tggctgccat gatcggccga tgcggcgtgg ccgtgcttgc gcgtcttgtc aatcccggcc
      541 cagccggcaa cgatgaggac ggcgaggaag agggccacga ccgcgccgag ggcgagccac
      601 gcgcgccgcg cgaggcggct gccgggggtg ggtgtcgaat tcatcgaagg ctttgtgttc
      661 atagcgcgtc gttcccaccc tgggaatcgg gcgtgaggag cgccgccagg cgagcctgcg
      721 cggcgtagcg gtcggcctcc cgagcggcca actcggcccg caggtcgatc catcggcgga
      781 actcgtcgag caccgacacg aaatcgccct gccccgccag gtagctcgaa cgcgtcgcgt
      841 cgagcgcggc gctgctctgc ggcagcaggc cgtcgcggta gcgtccgacc tgttgcagcg
      901 cgttgccata ctcgaccagg aggctcgcgg cctgcgagcg gacctcgagc gccgtcgagg
      961 ccagttcgag cgaggccgcc cgccgctcgc cctccgctgc ctcgaggagc ggctgctgct
     1021 tgcgcttctt ccagaacggc agctcgatgc cgacgttgag gttcaccatc cggtcggtgc
     1081 ccccctgcca gtacacgccg gcgccgacgc tccacgaggg tttcagctcg gcgcgtgccg
     1141 ccgcgacctg ctgtgccgcc gcatcgacct cgcgttggcg gaccgcgatc tccggcgcca
     1201 ggcgtgacgc ctcgtcggca agcgcttcgg gcacggcacc cggcagcgcg ggcgggggca
     1261 gcgccgacac cgcgccgatg ggggcgtcgg gcggctcgtc gaccagccgg ttgagcgccg
     1321 cccggacgat ggccctctgg gcctgcaggt cggcctggcg ttgccccagc cgggtgcgtt
     1381 cgagctggac ccgcagcagg ctcgcctggt cgctggcgcc ggccgcgtag cgcgccgtca
     1441 ccgtggactc gagcaggcgc aggatctgcg ccgcgtcggc cagcaccgcc tgctcgcggt
     1501 cgatcgcgta gagccgcgcg tactgcaccc ggacttcggt cgcaagactg gtcgcggaca
     1561 gctgccgctc ggcagagcga agcgacgcct cggcggtcgc gatcgcccgc cgcgcgccgc
     1621 ggcggcctcc gccaagcagt ccctggcgaa gcgacatccc ggccatcgac ccgggatcct
     1681 cgccgatggt gtaccgaggg aagttgaacg cctggtactc ggcctccacc atcggatcgg
     1741 gcagggcgtc ggcggcgcgg agggcgatgc gggcagcctc gaggcgctcg ccccgcgcgg
     1801 ccagcgacgg cgcgcgagca agcgcgcgct cgaccagggc ctccactcca ggggcttccg
     1861 gggcggtgac ggcggaaggc accgcctggg ccgcaactga agccggaagg gtcaacgcca
     1921 gcagtacggc gggcaggccg ggcacggaca aacgaatgcg atcgagcatg accatcatcg
     1981 acagctggaa gaaaggccgg ttgtctcgac gtgccggaaa gggcaagccc ggtgccagcg
     2041 gattggtggg gaagcgggcc ctgctgcctg gaaatccggc ccgaactcgc gaagacgcgc
     2101 accgcctcat cccgatggtg atcgatatga ggcagcccac cggggtcggt gcgtgatttc
     2161 agacaccggc acggcgacga agtcgggggc cggggcacgc cggggcttgc ctcggccgtg
     2221 ccgaaggtgc caacgtgaag ggatgtccga cccaaccccc gcgcggtggg tggcgcttct
     2281 cgcggccgtg acggctgtct cgacgctcca ctacgtgacg cccgcggcca gtccggccct
     2341 tcacgcgttc taccagcggc tgtattacgt cccggtgatc cttggcgcct attggttcgg
     2401 cgcgcgcggc ggcgtgctgg ttgccgcgct ctgctcgctc gcctacgctc cgcacatcca
     2461 cgtcgcctgg tcgcagaacg ccgcttacac cgcgagccag tacgccgagc tggtggtgtt
     2521 cctttcgctc gggctggcgg tgggcctcat cgcgacccgc gagcgcaggc tgaccaatcg
     2581 ctaccgcgag gcagccgagt cgctcgagca cgccaaccgc gagctgcgcg agtcgggcga
     2641 gcagttgcgg cgggccgaac gcctctcggc cctcggcgag atcgcggccg gcctcgccca
     2701 cgagatccgt aacccgctgg ccggcatgaa gggggcgctc gagatcgttg gggcgcgcgc
     2761 gaaagccggc acccccgagg ccgagttctc ggccatcgcc gcgcgcgagg cgcagcggct
     2821 cgaggggctg gtgcaggagt tcctcgacta cgcccgtccc cgtccccccc gccggcgccc
     2881 gggcgagctg ccgcaagtgc tgcgccgcgt ggccggcctg ctgggcccgg aggccgagcg
     2941 cgctggggtc gtcctcgaga tcgaggcgga cgccgcgccg tccggccccc cagtggatcc
     3001 cgagcagatc cagcaggtcg tcgtgaacgt cgtcctgaac gccatccagg cgagcccgcg
     3061 gggcggccag gttcgggtgc ggtcccggag cgaggcgcgc cgcaccatcg tcgaggtgac
     3121 cgaccagggt ccggggatgg caccggaggc gcgtgcccgc gccttcgagc cgttctacac
     3181 caccaaggag cgggggagcg gcctcggcct cgcgatcagc cagcgcatcg tcgtcgccca
     3241 cggagggacg atccggttcg aggaagcgac gggcggggga acgagggtcg aaatcgagct
     3301 gcccgatgcc ggcaccctgc cggcaggtgc ctgacatgac cgaccagaag acggtgctcc
     3361 tgatcgacga cgacgagagc ctgcgccggg tcgtggagta caacctgcgc gaggacggcc
     3421 acgaagtggt gacggcggcc gacggccgga ccgggctcga acgcttccaa gcgtcgccgg
     3481 ttgacctggt cctgaccgac gtgcggatgc cggagatgtc ggggctcgag gtgctcgccc
     3541 tggtgaaggc gatgcagccc gacctgccgg tgatcgtcct caccgcgcac ggcaccatcg
     3601 acgcggccgt cgaggcgatg aaggccggcg ccttcgacta cctgacgaag ccgtttaccc
     3661 gcgagcagct gctggcctcg gtgcggaagg cgctggagct gggggcgctg gcgaccgaga
     3721 accggcagct gcggcagatc gtgcgcgagc gcttctcgtt cgcgaacctc atcgccggat
     3781 cgcgcgcgat gcgggcggtg accgacacgg cgtcgcgtgt cgcggcgagc gacacgaccg
     3841 tgctgctgga aggggagagc ggcaccggca aggagctgct ggcgaaggcc atccacgtga
     3901 acagcgcccg gagccgcggg ccgttcgtca ccgtgaactg cgcggccatc cccgagcacc
     3961 tgctcgagtc ggagctgttc ggccaccggc gcggagcgtt cacgggggcg gtcgccgaca
     4021 agcaggggaa gttcgaggcc gccgcccggg ggacgatctt cctcgacgag gtgggggagc
     4081 tgccgctgct gctgcaggtg aagatcctga gggtgctgca ggagcgcgag gtggacaagc
     4141 tcggcgagac gcgccccgtc aaggtggacg tccgggtgat cgcggccacc aaccgcgacc
     4201 tcgagaagat gacggccgac ggctccttcc gcgaagacct ctactaccgc ctggccgtcg
     4261 tctcgattcg cgtgccgccc ctgcgcgagc gggcggacga cattcccctg ctggtcgacc
     4321 acttcctcga caagcacgcc tcccggttgg ggcgcgagcg gcccgccgtc gacaaggcgg
     4381 tctatgccgc cttcaaccgc tactcgtggc ccggcaacat ccgcgagctg gagaacgtca
     4441 tcgagcgcgc gctggtgctc gaccgcgacg gcgtgctcgg cctcgacgat ctgcccgagc
     4501 gcctccaggc cagcgggcag cggatcggca acctgcggat cgagctgccc gacgagggga
     4561 tctcgctcga ggacgtggag cgcgagctgc tgctggcggc gctcgagaag cacaactgga
     4621 accagacgcg cgcggctgcc tacctgaaca tcacccgcag cacgctcctc taccggatgc
     4681 agaagttcgc gctcgaacgc cccaagaccg agccctgacg tgttcttcgt ggtcgatcct
     4741 tttgtggttg atcgcttcgt cacgcacggt atgcttggcg gtgcgcccgc aagggcgcgg
     4801 ggaggccttc gatggtcgcc gtcgtcgcgt tcgccgctgc cctcgtcgtc ggttccttct
     4861 tcgagtacgt cgtgcaccgc ctcatgcacc ttcggtgggt actgggcgac cggcacctgc
     4921 agcaccaccg gaagggatgg gggcagggtg tgctcggtga gttccgcgat tacctgtacg
     4981 gcgtcccggt catcgggtgg ctcgggtttc cccattcggt cgaggccggc atcggtttcg
     5041 ctgccggctg tgtggcttac gccttcttcg ccggctacag ccaccagctc aatcacgagc
     5101 ggcccgagct cgtgttctgg ctgccgcggc cggtccacca cctgcaccac gcgcaccggc
     5161 agtggaagca gaacttcggc atcggcgtgg acatctggga ccgcctgttc ggcacttacc
     5221 aggtggtgga gtggaagccg gaacggcggc ccttcgacca ccccctccgc gaattcgggc
     5281 ggatctggtg gagaaagtac aacggaccgg agggacggtg acgacccgcg ctgatccacg
     5341 aagcgccatg tcccgaaccc cgaatcccga atcctgagtc ccgaaccctc aatcccgaat
     5401 cctgatcgcg tgatatcgtc cggtgccagg accaagtcgc ccctcgcgga ggtttcgatg
     5461 cgatcggttg tcctggttgc gatcatgacg tggctggccg gcgtcccgct gtgggggcag
     5521 gggccgtcgg gcgggtcctc ttcactgagc tggttcaagg gaaacactca cacccacacc
     5581 ctgaacagcg acggcgacag cacgccagac gaggtcgtgc gctggtaccg cgagcggggg
     5641 taccacttcc tcgtggtgac cgaccacaac ctggtcacgc cggtggacgg cctcaatgcg
     5701 gttttcgcat tcgaaggccg attcctcgtc attccgggcg aggagatcag cgacgtcgtt
     5761 cccagcgggg cgggcgagga cgcgcccggc atcccggtgc acgtcaacgc gttggggacc
     5821 cgcacggcga ttcttcccgc cgggggcgcg acggtggccg aggcgctgca gcgtgacgtc
     5881 gccgcggtgg aagcagccgg ggtcccggtg cagatcaacc acccgaactt cgggtgggcg
     5941 atcgcgcagg ccgatctacc gccatcgtcg cgaccgctgc tgctcgagat cttcaacggt
     6001 catccccagg tgaacaacct tggcggcaac ggtacgctgc cggccgagga gttatgggat
     6061 gtctggctgt cggcggggcg aaaggtcttc ggcgtggcga gcgacgacac gcaccacctg
     6121 aagcggccgt gggaccggcg ggcggcaagc ccgggccgcg gctgggtcgt ggtcagggcg
     6181 gccaccctca gcgccgatac catcctcggg gcgctcgggc gcggtgagtt ctacgcgagc
     6241 acgggggtgg agctggccga ctggcaggtc agcccggaag gcgtctcggt cgccatcgcg
     6301 acgcggcccg acgacctgac gcgcttccgc atcgagttca tcggcaaggg cggccgcgtg
     6361 ctgcgccaga ccgacggctc cgacgcgtcg taccagttca cgggcgacga gggctacgtt
     6421 cgcgtccggg tgaccgactc gaacgggcag cgggcgtgga cccagcccta cttcctcttc
     6481 tcccgcgcca gcgcgcggta gtactcctcg acgagcgcgc ggtactccgg cgggacgtcg
     6541 tcgctggccg acagcagcac ctcgcccccc tcgtcgaggc gccgccgcag gtcgaactcg
     6601 aggcggcgga agccctccac cagctgggcc tgcaggcggg ccagggccgc cgggtcgtcg
     6661 tagaggcggt cctggtcgag cgcgcgcagg tcgcggatga gggcgtccag ctcgcgggga
     6721 ttcagtccaa gagcccgcat ctcgcgccgc agctgctcgg cctcgctgcc gcgctcgcgc
     6781 gcctcgcgcc gcagctgccg cgcctgctca gggctcatgc ggcccgggcg ccccccgccc
     6841 ccgccgccgg cctcgggcgg cacgccgacc gagctgccct cggcccgctg ctgatccccc
     6901 gcctgcccct gctggccgcc ctgcgcctca cccccctgcc cgccgcccgc ggtaccctgc
     6961 gaggcctctt gcccctgctg ccgaccgccg tcggccccct gctggctgcc gtcggcctgc
     7021 tgtccctgcc gcccgttccc ggcctgctgc cggctgccgg gtgccggccg gctgccgtcc
     7081 gctgaccgct gctcgcgggt ccgctcactc atcgattcga cgccgcgcag caggtcgcgg
     7141 gcacgctcga gggcttcggc gcgcgtgtcg cgcgcgccgc ccgatgcggc cgccttcgac
     7201 gcctcgtcga gccgccctcc gagcgcggcg agatcggcgc ggatctgctc ttcgaaagtg
     7261 gaggcctgat cgggaggggc gttctggatc aacccccggg aataccggat cttctccttg
     7321 agccggctgt ctcgaatgcc ctcggcggct tcctgcagct tgcgcgcggc gtcgcgccgc
     7381 tcgcgcccga agtcggaggc tgcgcgatcg agcccgcgtt cgaggtcggc cacctcgcca
     7441 gcctgcgcgt ccttgcgttc gccgagctga cgtgcctgcg cctcgcgccc ggcccccgta
     7501 cccggcaaac cgcgcacctc tccctcgatc cgttcctgct cctgctgcag gcgctgtacg
     7561 cgggcgcgcg cctcgtcgat cgtgcgcgtg agccggtccg tccgatcgct ctcgaggccg
     7621 cggcgcgcgc gtcccagccg ctcggccgcc gcgcttgcct ccgcgaagcc ggcccgatcg
     7681 ccggaggctg ccgcacgccg catggcgtcg gcggcctgct gcatctgccg cgccgcctcc
     7741 gcgaggtcgg ccctgcgctc ctcgcgcgcg aggcgctcca gccggcgcgc cgcctcctcg
     7801 gtctcggcgg ccagctggcg ttggcgctcg gccgccgccg acccgccgcc gctctcggcc
     7861 ccgccagaca gccggcgctg cctctcggcc tcctgttcct ggcgccgggc gaggtcgcgc
     7921 agccgctcga gcatctcgtc cacgcgctcg ttcgacgact gctggcggct gcggcggaag
     7981 gtctcgtact ggttcttgag ccggtcgagt tccatctcga acagctcggc cagctcttcc
     8041 tgccgttcgg cggagccgcc gccgccctcg ccgccgcgtt cctggctcat cgcgatccgg
     8101 acgtcgcgga aggcctcctc tgcggcctgc agcgatcgca gcgcccgctg ctcgggggcc
     8161 agggcgcctt tcggatcgcg cgcctgcagg cgcttctccg cctcgcgcat ttccttggcc
     8221 gcttcaacga gccccgcgtt caccccctcc atgccgccgg cgccgcctgc cccgagccgc
     8281 gacgcgatct gcgccgagag cgtctccacg cgcttgcgca gctgcccttg ggcgaggccc
     8341 aggaacacca cgtcctcgcg gaacttcgtc gcgcccacct tcgcgcggtc ccgcgcgacg
     8401 ttgaacgtgc cggtgacgat gcggcgctgc tcgtcggaga gagccgacgg gtcctgcgcc
     8461 ccctggccgc cggcctgccc gccgccctgg ctctccgcgg cgcggtagtc cttgcggaac
     8521 ggccggatgt gcaggaagta gatgtcgctc gtcgcggtct tcgccccggc aatcgcgtcg
     8581 gtgtcggtgg cgcgcgcgta gtacgacacg acatcgcccg gctgcaggtt catttcctcg
     8641 aggaagaacg tgtggccggc ggtgagctgt ctcgatcgcg agccgccccc cagcctcagg
     8701 ctccgctcgg cgcccccgtt gaccgacacc accaggtcga gcctcgcgat cccgaagtcg
     8761 tcctctgcgc gagcctcggc gaacacctcc tcgaggctcg tcggcctcag gtcgcgctgc
     8821 ggccgcgcga tgacggccga cgggggctga tcggcgagga cgtcaatcgc gtagtgcgga
     8881 gacgccgcga ccatctcgcc ggtcggcgcc tgcaactcga cgtggtacga cccgttgcgc
     8941 ctcacctcga actcgccgtc cagcgcggtc tcgagcgcgt tcgcgagcgt tgccggctgg
     9001 cctccctcga cgacgatccg gcccgacgag gccggcagcg tcgagtgcac ccgcacgcgc
     9061 actttcgttc cagccaggac agcgatgtcg ccgccgttct cgaccttctg cggagagagg
     9121 ccggtgtagg cggggaagac gtactccagc tcgaggcgct cgacctgcgg caggtcggcc
     9181 acctccacct tgtagaccgg cgaacgcacg ccggccgcct cgacgaagta ctcgatcgag
     9241 tcgcgcagcc ggaacaccgt ggcctcgaac ccgtcgcccg ttgcggggat catcgtcgct
     9301 cgctcgaagc cggcgcccgc cgccgcgcgc gtgaagagcg ttccttccgt gctggtgaaa
     9361 ccgacgaggc gcgccgagac ggcgaggtcg gcgccccgcg ggagcgtcgc ggcgccgggc
     9421 gcgacctcga tgcggtaggg cgacgaagct tccaccccgc gaaccggaac gagcagcgtc
     9481 attgccccgt gccgcaggta cgacggaccc ccgaggaaca cgatcgccgc cgccgcgccg
     9541 atggcggcaa ggccctgcca cgagcggcgc atgctgccgc gctccagctc gcgcccgtca
     9601 cggatccgct cgcatcgctc gacggccgag tggatgagcc gctcgagcag cgcctccgaa
     9661 tcggtgcggc cctcgcccgg ctgccgcaag ctgccgtcca ccgcgctcag cagcagcgcc
     9721 tcgagcgaag gctcgtgctc ttccaggtaa agggcgacct ggagatctgt gacgcggcgt
     9781 gcgaggggcc ggaccaggaa gaaccagccg agcccgacaa gcgtgaggta ggtcaccacc
     9841 cggaaggcga cgatcgcctc gggacggaaa cgccagcgct cgaggcccca cgcggcggcc
     9901 agcacggtga tcacggctgc gcccagcagc accgtgaggc tgcggagcac cacgcgccag
     9961 cgccagcggc cgcgcacctg tcggatcacg cgcaggagct tgtcgcgttc gctcttgtcg
    10021 tgggggggca taacgtctcc atcacaaaca gctgacagcc gacagcggac agcaacggaa
    10081 gcggacgcgg acgcgcgacc cgctctcgta ggggcgaccc ttgtggtcgc cccgccatcg
    10141 gatcacgacg ccaggcgctg gctttcctgc gctgccgcgg cactccgttc cgcgacctcc
    10201 gccgcggccg gcacgggacg ccggcgcgag aagcggccgg cgagcagcgc ttcggctcca
    10261 agggccagca cggccagcgc gagcaggtac caccagagcg cctgtcgccg ctcgcggtcc
    10321 tctggcgaca gttgccgggc ctcgctggcg gccgcgacac cgggtccctg cctgaccgcc
    10381 gtcacgagct cggcagggtc gagcctggcg aggttcgatt cggcgggatc cacgttcacc
    10441 gccagcaccg accgctctcc gtcggctcct tccccagccg ccgctcgaat ctcgtagaac
    10501 ccctgctcgg ccggcacgaa cgcctgccgg ctcccctcac cgggaacggg aaggcgcgcc
    10561 ccggacggcg acagcgcgac ttgcccggtg cgcgttcgcg aggccgcctt tcccccctcg
    10621 gcaccggctg cgagcatgga cgggctcgcg gcctcgccgg cgacgtgcca ggcgggccgc
    10681 tgcacgtggc ctgccaggta cttgatcgcc tggtgcagga gcggcacgaa gacgggcttg
    10741 agcaccaggt cgttccagta gccgtcgagc gtggatgtcc aaacgaggac gcgcccgcgg
    10801 ccaactcgcc gctctgccac cgccaccgcg ccatcgtcga accgtgcgag cacgcccgcc
    10861 ggcgccgcca gttcgcggaa gcggaagacg cgcgccgccg cgaggtctcc gctgcgcggc
    10921 gtgccgaaga cctcgaaaac cgggtgcgag cgttcgacgt gtccgatggt ggcgccgcgg
    10981 ccctcgcgcc tgtccacgat ttctccgagc gtgccgggca gcaggtcggg ggccgcctcg
    11041 ctccaggacg agcgctcgcc gagggcgagc agcagtcccg tgccccggtc caccgcggcc
    11101 gccagcgcgc gcccggcggc gccagcagga ggcggagtgt cgttcagcac gacgacggca
    11161 gcccgctcga tctcggctgg cgttgcctgc gcagcggcga ggaaccgagc ctcgaaccgc
    11221 ggctccgagg agaccgccag ggcacgcgcc aggaagggtg tctgacccgc ggccgtctcg
    11281 acgacgagca ccgggagctt ctcccggctt gccagcacgg cgtgaaacgt atcgtcgaac
    11341 ggcagccggt cgggcgtcag ccgtgcgaca acccgcacca ggccgttggc cggcagcgta
    11401 aggggcgaga aggaaacgct cgccgtcgtg ccgggctccg ccgagacgcg ggcggcgtcg
    11461 agctgcctgc cgtcggcctc gagggcgatc ggcctgttgt cgatccgggt cgagccgcga
    11521 ttggccacgc gcgccgtgac agtcaccctc tcgctgcccg actggtccgc ggcctcccgc
    11581 gcgaaggcga ggtccaccac ggcggcgttc tcgacgcgcg cctcgccgac gggcatggtc
    11641 gtgagagcga ttccaggggc caccttcacg ccctgccccc gatcccagcc cgtgccctgg
    11701 aagtcgctga tcaagacaac ctcgcgccgc ggcatcggcg acgactcgag caggccggct
    11761 cccgcctgga ttgccggccc gtagcgggtg gcgagggcgg ccggccgggc cgcctcgatt
    11821 gccgccagga gggcggagcg ctcgcccccc tgccgcacgg caatctcgac gtcgctcgcg
    11881 aacaggacga gcgatgcccg gtcgccaggc cgcaggccct ccaccacgct ccgcgccgcc
    11941 tcgcgcgcgc gctggaagtg gtcgccgtac gccatgctcg cggagcggtc gagcaggatc
    12001 gccacctccc gatcgccggt tcccccggcc gcggcgtcgg cgcccggcag gaagggccgg
    12061 gcgaacgcga ccgccagcag cgcgagcacc agggcgcgca acaacagcag cggccagtgg
    12121 cggatcacgc ggcggcggac cgactgatac gggatcttcc gcaggaacat caacgagggg
    12181 aacgccacca cctgcttgcg ttcgcgctgc acgaggtgca gcacgaccgg gatggcgatg
    12241 gcgagcagcc ccgcgagcag cgccggcgcg aggaggctca tcgcaccctc ctgaggcgct
    12301 cgcgggcagc caggtaccgg aacagggcga agtcgagcgg gaccgacgtg tcgagcagtg
    12361 cgtagtcgac ccggctgtcc agcagcaggc ggcccagggc ttcgatgtgc cggcgcacca
    12421 gctcctggta ctcggcccgc agcgcctcgg gaacgaccgg cagccgctcc ccgctctcga
    12481 ggtcctcgaa gctcgaagcc tcgtcgaacg ggaagtcgcg ctcggccgga tcgagcaggt
    12541 ggaacacgat gacgtcgtgc ccccggaacc ggaggggccg gacggccgcg gccacccggt
    12601 cgggctcctc gtagagatcg gaaaccacga cgacgatccc gcgccgcctc aatcgctcgg
    12661 tggcggtcag cagaggggcc gccagctctc cctcgcggcc ggcctcggcc cggtcgagcg
    12721 cgaagagtgc gaagtcgagg tgcttggccg acggcggcac gtagctgacg atgcggcggt
    12781 cgaaggtgac gaggccgacg cggtcgcgct gctggtgcga gaagtagagg agcgacgcgg
    12841 cgaggtaccg cccgtagtcg atcttcggca ggccgcggct gccgaagcgc atggaggccg
    12901 acacgtcgag cacgacgacg aggttggcgt tcgagtcggc ctcgaactgc ttgacgtaga
    12961 agcggtcggt gcgtgcgtac aaccgccagt ccacgcgccg caggtcgtca cccggctcgt
    13021 agccgcggtg ctcggcgaag tcgagcgaaa agccgaggtg cggcgcgcga tgcaggccgt
    13081 tgatgaagcc gtccacgacg gtgcgcgcca ccagctcgag gctgttgagg cgcgccagca
    13141 ccttcgggtc cacgaacctg gcgcccgcca tcgcctgcgt ttgctccacg gttcttccct
    13201 cgcctccagc accggaatcc cgaatcctga tccctgatcc ccgatccctg atccccgtct
    13261 acatccccga cctcggcacc ggcactgcct cgagaatccg atcgatgatc tggtcggtgg
    13321 tgatgccctc cgactcggcg tggaagttgg tcagcacgcg gtgacgcatc accgggtgcg
    13381 ccagggcacg cacgtcctcg aaggtgacgt ggtagcggcc gtgcgtgacg gcgcgcgcct
    13441 tggcgccgag gacgaggtac tgggcagcgc ggacgcttgc cccgtaggag atccacttct
    13501 ggacgaaatc gggggccccg ttcccgcgac ggctcgcacg gacgagcgcc agggcgtagc
    13561 gcatgacggg atccgacaca gggacgcgcc gcaccaggcc ctggaacgcc acgaggtcgg
    13621 cggcgccggt tgcccgatcg aggcgcacct cgcggccggc ggtcgtcgtc ctgagcacct
    13681 ccagctcctc gtcctccggc gggtgttcga tgacgacgtg gaacatgaaa cggtcgagct
    13741 gcgcctcggg cagcgggtag gtgccctcca gctcgatcgg gttctgcgtg gcgaacacgt
    13801 ggaacggctc gtcgagcggg taggtgcgcc cctgcacggt cacccggtgc tcctgcatcg
    13861 cctcgagcag cgccgcctgc gtcttcggcg gcgtccggtt gatctcgtcg gccaggagga
    13921 tgttggtgaa gaccgggccc ggctggaaca ccatccggcg gccgccgatc tcggggtcgt
    13981 cctggatgat gtcggtgccg gtgatgtcgg acggcatcag gtcgggcgtg aactggatgc
    14041 gcgagaactt caggtcgagc acctcggcca tcgtcctgac gagcagcgtc ttggcaaggc
    14101 cgggcacgcc gacgatcagg ctgttgccgc cgacgaacag cgtcagcagc acctggtcga
    14161 ccacctcggc ctgccccacg atgaccttgc ccagttcgct ggcaatcagc gagcgcgtgg
    14221 ccgtcatgcg ctcggcaacc gagacgtcgt cctgcgactc gagaacgcga acatcttcgt
    14281 tggcaatcgt cacgctgctc tccggtgggc ggcgcttgcg ccgcccggtt gatcgtccgg
    14341 caggcacgtc tccagggcct gcccggctca gtgcgtcatc ccgtacacca cgtagttcac
    14401 gccgaacttg taggcctcgt tggtcaggtc gatcgggttc cacccggtgt ccgaccactc
    14461 ccagtactcg ctgatgtcgt tgttgtagtt gacgatcgcg agcatccgct tgcccgggtc
    14521 gttgccggcg aagatgccgt agaagacggg cggttcgcgg tcgtagaact ggcggaactc
    14581 gagcgacggg atctcgaaga acgagtggaa cacctggctc gccggttcga gctcgatcag
    14641 ccgcatgccg ggcaggaccc gccgcatttc ggccgtgaag ttgtcgaggt cgcggccgcg
    14701 gaagtcgtcg aagatcagga agcccccctt ctgcaggtag gcgcgcaggt tggccgcctc
    14761 ctcgtcggtc tgcgtccaga agccggcctc gcagaagtag gcgatcgggt agcggaacag
    14821 ctccttcgac ccgatgtcca cgatggcgcc gccctcggcg ccgtcgtagg tcctgatcgt
    14881 cgtgatgcgc ccgagcagtt tcatgaagtt gcgctcggcg cgcgggtagt cgtgcgccca
    14941 cggcggctcg cgcgacccgc gccgtccgaa gccaccctcg aaaccgccgg ttcccaccgt
    15001 gtaccggatc cgcgcgatca cgaaccggcc gtcgtggggg atcgggacgg gctcgggttc
    15061 ggcggactgg ccgcggccct cgccccacag gccctgcagc agccgccgga tgcgcgagcc
    15121 ttcggggctg cccggctcgg cctgcggcat ggcaaacgcc gccacggtcg ccgctgtggc
    15181 gaacacgatg gcgcaggtgg cgcgggctgt ccggctcatc ggttcccctt ctcccgctcg
    15241 cggatctcgc gcgtcttcct ggccagctcg aggagcaggc tttcgagctc ctgctggtac
    15301 tgcgccggcg gcatgccgtc ccgggtcgcc ttcaactcgc cgatgcgccg ctcgagcgcg
    15361 ccgcgctctt cgtacagggc gagcaggcca gggtccgcgt cggccggcgg cccggctctc
    15421 tccgctcccc cggcagacac gaagactgtc cgggccagcg cgccgtccgc gccgtcagac
    15481 ccgggggccg cgcttccctt gccgtcaccg tcgtcgtcga ggagcgcgtg ctcggtcagc
    15541 agctgaccgt cctgctcgta cgaggcggcg gtgcgctgcc gcgcccaggt gaaggcttcg
    15601 agcatcgata cccggccgtc cttgtcgagg tcggcatccg tggaggcgag cgcctcgacg
    15661 aagaagcccg ggaaccgtgt ctggttccgc tccccgtcgc ttcgcgtcgc cgtgatcacg
    15721 acccggttgc ggcccgagag cggcccgagg aacgccccgc tcgcgctggc agtgttcacg
    15781 aaggccaccg ttcgcccgcc gaggcgatcg aggaggcgcg cgaagtcggc gggcgagagg
    15841 tcggggccgg gcaggttgaa cctcggcggc cccgatgccg ccgccccgtg gccaatcagc
    15901 acgatgagca cgcggtcgcc cgggcgggat cgcgacgcca gccgcccgac cgcgttggcg
    15961 atctcctcgc gcgtcgatcg gccgccgatc cggcccgggt cgcgtgccgt gtcctcgccc
    16021 aggtagacca ggctctcggg tgggatgccg ccccgttcac gcgcggcgtc cacgagcgac
    16081 gacgcccact cgtggaagcg cgcgcgattc tcttcgtcgc cgccgaggcc gacgatgacg
    16141 agcaggtgcg tggcctgggc gatggccggc gtcgagacgg ccagcagcac gagggggagc
    16201 atcgttgcga gccgcctcat gccagtccac tccgccgcct gaggaaccac tcggcgccca
    16261 cgagccccac gaggagcaag agcaccgcgg gcatgtccca gagatccatc tctctcgtcg
    16321 ccgtgacgcc tcgcccaagg taggttgcgt cctcggcgag cgacgcggcc gtctcgggcg
    16381 tgtagtagcg tccgccggtt tcctccgcga tccgcttcag caggggcgcg cgctgcgcgg
    16441 cgtcgaagta ctccgcgtcg ccgggtgcgg cgcgcaggta ggcgacggca gcgcccagct
    16501 ggtcgcgtcc gcgtgccgcc tcgaggcgca cggtgtgaag acccgcggcc gccggcacgt
    16561 acgacgcggc gtactcccct tcgcgatcgg gcgcgaagtc gagcggcagc tcggtctcgc
    16621 gaccggacgg gtctgtgatg cgggcgcgga cggaggcgtc gttgacggcc aggaaacggg
    16681 catcacgcac gttcgccgcg actcgcaccg cctcgcccgg ctccacccgc tcgcggtcga
    16741 gcgacacctc gacacggtcg ggcacccctt cgacgagcca acgggcgagc cgccgccaga
    16801 aggtctcgtg cgtctggtcc tcgaccggga tcgaggcgtc catctgccag agccacgagt
    16861 cctggaccgg gaaggcgagc gccttgccgg cgccgtagcg ctggtgggcg agcacgacgc
    16921 gctcgccctc gccctcgagc agcgcggcgg cgccgggctt cagcgaacga atcgggttca
    16981 ccgtggtgag cgccggcagc gtcttccatc gcgccgcgga cgtctcctcg gtcgcggcca
    17041 cctgcgtgac ggcgtggagc tggcccagcc gggtcgggcg cgccgacaca gtctcgtggc
    17101 gatcgtcggg aagcgggggc gcgtcgaggc gcaccggcag cgcctcggca accggcgtgc
    17161 ccgcgtagcc tccctcggcg aaggcgtgcc ggcccccgag cgcgagcagg cctccgccgc
    17221 gcacgttgac gaagtcggcg atcatccgga gctggtcggg ggtgaacgcc gccgcctcga
    17281 tgctgccgag gacgaggccg cggtaggcga acagctcgtc gcgcgtcttg ggaaagccgc
    17341 ccagcaggtc gtcggggccg tccacgtcga ggcggaggaa cttccgctcc gccgtgcgct
    17401 gcagcgtcac cacctgcagg tgctcgtcgc cggcgagcga ctgccgcagg aacttcatct
    17461 cgaagcgcgg ctcgccctcg acgtagagga gcttctcgcg ccggtcgcgg acgtccacca
    17521 cgacctcgcg cgcgttgttg cgcgtcacct gctcgccgtc gagcggcggg atcgtgaagc
    17581 gaagcaggcg gggcccggcg tcggccagcg tcagccgcac gcgcgcggtg accggctcgc
    17641 catcgcgcgg cagcagaacg tcggccgacc cgacgcggcg cccctcgtcc tcgacgagca
    17701 gcggcaccgt gcggcccgcg tagccggtct gcgcgacgga cacctgcacg attagcgtgg
    17761 agccctggag aacccaggcg ggcggctcga cgcgcccgag ctggacgtca cggtcgaacg
    17821 cctcgcggcc aaggccgatc gtgaacactg gcagccccgc cgcctggatc gaccgaagca
    17881 tcggcgagat gtcggaggcc gaggtgtcgg cgccatcgct caccagcacg aggcccgaca
    17941 ccggcaggcc cgccagttcc gacgcggctc gcccgagggc ctcgcccagg tgcgagcgcg
    18001 tcccgtcgaa ggcgagcgcc tcggggcccg gcacgcgttc ggtcgaggac gacacacgga
    18061 agaaccggag ggcgaaacgg tcgccgagcg cccggacgag cgatccatcg tgaccgaagg
    18121 cgcttcgcac gaatgcgctg cgcggcaccc cgtcgcggtc ggagatgctc atgctctgcg
    18181 agtcgtccag gaggaccgcg aggaagttct gccgtgcctc gactgccttg accgtgacgg
    18241 ttggcctcag gagggcgaag aagagaaccg ccagagtcgt gccgcgcagt gcgagcagca
    18301 gcaccgcgcg gccggaaggg cggccgtcgc gatgccaggc ccagagggtg aggccggcag
    18361 cggcaaggac cagcagcacg gccgccgccg ccggccaggg cggactaaag gcgaagccgc
    18421 cttgctcgaa gacgaccggt cggtacttga agagcagcgc gaagaggtct tccgccacgt
    18481 cgcccccggc cagcccatgg ccggggaggt ggccgtggcc ggcgcccaaa tttcgattgt
    18541 ctaagcccaa ttcggaggat agcacgaccc gaccgcgggg aggccggggg ctataatcct
    18601 cgtaaagggc tcatgctcaa ccatatggga ccggaccacc ggcgtggccg gaggggattg
    18661 gccgcctttg cggttgcggg tgtgctcggc ctggcagcct ggctggccgt cgacacggcg
    18721 ggtcgccggg agccggcgca cgcgatggct ccgcagcgcg gcggaggcgg cgcggccgcg
    18781 gacgcgccgg cagccttctc gcaggaggtc gcgagggcga tcgcgcaagg gcgtcgccag
    18841 gaggccgagg cgatggcgcg cggccgaggc gcggacgatc ccgatgcggc ggccgcgctg
    18901 gctcgcctcg tcagcgaccg cggcgactac gaccaggcgg aaacgctgct gagagccgcc
    18961 tcgcggcatg cgcgcggcgg gcaggccgcg ctcgaactcg gcctcctgct ccagttccgg
    19021 ggccggcccg atgaggcgcg cgcggtgctc gagccccttg ccagtggccc ggaaggctcg
    19081 gatgccacgg cggcgctcag ggcgggggtt gcctcccgag cgctgggcct cgcccacgaa
    19141 gccaacgagt ggctgctcga tgcggccagg cttgcccccg gcgaccccgc tgtccacgtc
    19201 gcccgcggcg agctgttcct cgagcggcac gaccgcgacg aggcggcctc gctcttccgc
    19261 caggcgctcg agcgggacgc gcgctgggcg cccgcgcacc tggggctcgc gcgcgcgctc
    19321 gccgacgaga acacgccggc cgccgaggcg gctgcgcgac gtgcggagga gatcgaccca
    19381 gggatggccg gcgccccctt gttacgcgcc gaggcggcgc tgcgcgccga ccggctcgac
    19441 gacgggaggg cggcgctcga tcgcgcgctc gaaatcaacc cgcgcaaccc cgcggcgcac
    19501 gcgttggcgg ccgccctggc gcacctcgag gggcgggccg ccgatgccgg cgcgtctgtg
    19561 gaggcagccc tggccgtgaa cccgacctac ggcgacgcct tccgcgtgcc ggccgagcag
    19621 ctggcgtcga gcggccgctt cgaggacgcg gtgccgctgg cccggcgcgc ggcagcgctc
    19681 gaaccgtgga acacgcgggc gctcgccgac cttggcatgc acctgctgcg agtgggggag
    19741 gaggccgaag cgcggccggt gctggagcgg gccttcaagg ccgacccgtt cgacctggtc
    19801 accttcaacc tgctccagat gctcgacacg ctggaccagt tcgtcacggt gccggcgggc
    19861 gtcggccgga tccgcctgga tccagccgag gcccccgtcc tccagccgta cgcggttcgc
    19921 atcgtcgagg aggcgctcgc gcgttacggc cagaagtacg ggctgcagcc gcgggggccc
    19981 gtcctcgtcg agatcttccc caagcacgat cacttcgccg tgcgcacctt gggtatcccg
    20041 ggactgctcg gcgcgctcgg cgcctgcttc gggcgcgtgg tcacgcagga ctcgccgcgt
    20101 gcgcgcccgc cgaacacgtt caactggcag gcgacgctgt ggcacgagct ggcgcacgtc
    20161 ttcacgctgc agctctccga ctaccgggtg ccgcggtggt tgagcgaggg gatctcggtc
    20221 tacgaggagg gacgcgtccg ccccgagtgg gccggcgact cggagatgac cttcgcgcgg
    20281 gtgtacgcgg cgggcaacct gccgaagctg gttgacttca acgccgcgtt cacgcgcccc
    20341 gacctggtgg ccgctgccta cttccaggcc tcgctggtgg tgggcctgct cgtcgagcgg
    20401 cacggggagg cggtgctgcg cacgttgatg tcggcgtatg cggcgggtgc ctccacggag
    20461 gaagcgctgc ggaaggccac cggcgccggc ctctccgact tgcagtcggc cttcgacgcc
    20521 atggtgcgcg agcgctacgg cgatgcggcc cgcgccctcg aaaccccgaa gaacgtcgag
    20581 atcccgaggt cggccccggc cgctgcgctc gtcgagatcg cggacaggca cgcgaacagc
    20641 ttccccgtgc aaatggcggc cggcagggcg atggcggcgg ccggtgcgca cgcggaggcc
    20701 atcgcggcgt tcgagcgcgc ggcgcgcctg gtgccgttcg cacgcggcga cgacagcccg
    20761 cgagcgcatc tggcgcggct cgccctcgcc ggcggcgaca ccgcgcgcgc ggcgagcgaa
    20821 ctggaggcgc tcgtccgcca cgaccacgcc aacgtggacg cggcccggca gctggcgtcg
    20881 ctggccgaga aggcggggaa cgagccgctg cgcctgctcg cctacgagcg gatcgtggag
    20941 gtcgatccct tcgacgctgc cgcgcacgcc gcgctcggac ggatcgccat ggggcgccgg
    21001 gacgccgccc tggccgtgcg agagttccag gcggcactgg cggccggacc cgtggatcgc
    21061 gtcgccgcgc actgcgacct cgcggaggcc tacctgctgg gggggcgatc cgccgacgcc
    21121 cgccgcgagg cggtcgcggc gctcgagatc gcccccacct tcgagcgcgc ccaggagttg
    21181 ctgctgaaga caacagaagg gaagtgacgc ggcgggagcc ctctcagtcc ctgtacacgt
    21241 ccgtgttcac gtacttcagc cccgagtcgg ccatcagcgt gacgaccgtg gcgccctgcc
    21301 cgagccgctc cgcgacacgg atcgcggcga cgacgtcggc gccggaagaa gtgccgccga
    21361 agagcgcttc ctcgcgcgca agccggcggg tcatctcctt ggcctcgccg gtcgagaccg
    21421 tgtggacctc gtcaacgagc gacgggtccc agagcggcgg ctcgtagccg atccccaccc
    21481 cttcgatctt gtgcggcccc ggctgacccc cggagagcac cgacgactct gcgggctcta
    21541 ccgccgcgat caggatgccc ggcttgtgcc gcctcagcac cgtcgcgacg cctcggatgg
    21601 tggccgctgt gcccacgcag tggacgaacg cgtccacctt gccgttggtc tgctgccaga
    21661 tctcctcggc gagcgggtgg tagccggcga ggctgtcctg gttgttcagc tggtcggtcc
    21721 agtaggtgcc gggctcctcg ctcaggcggc gtgccgtctc gatcatgtcg aggatcagct
    21781 tcctggtggt caggccgccc tcgctcggga cgagcgtgag ggtggcgccg agcgcccgca
    21841 tctgatcgag cttctcgcgg ctgaacgcgt cggagctgac gatgcgcagg gggtagccct
    21901 tggcggcgca caccagcgcc agcgaggcgc cggtgcttcc gccggtgtac tcgagcacgg
    21961 tccagcccgc ccggagccgg ccatcctctt ccgcccggga gatgacggcg cgggccatcc
    22021 ggtccttcac gctgccggtc gggttctccc attcgagctt gacgaagacg ctcgcgcagc
    22081 cgggcggaac gacctttcga aggcggacga gcgacgtgtt gccgaccgcg tcgaggatgt
    22141 gcatcgccat gatcaaggcc gacgtgacgc gaggtgtcaa cgactatccg gctggagcac
    22201 ggggcttcag ccccgttcct gccggagtga acatgatcaa accagctcgg cgccgtgctc
    22261 gcgcagcagc tcgcgcagga ccgagcgatg gcagcgcgcc tcgtcctcgc agtagcagcc
    22321 caccgcgagg ttggtccggt gcgagagcgc cgccagcagg tcgagcaggc gggccgcctc
    22381 gggccgcttc atctcggccc ggtagtgccg gacgaactgg cgccacgccc gctcgtcccc
    22441 ggcctgctgc gcctccttca caagtccctc ggaaggggcc aagtcgggca gccagacgtc
    22501 gtagaagtcg cgcgacgcgt gctcggattt cggcacgccg cgcggcggcc gccgcaccgt
    22561 cccgacccgc aggccctcgc cgggcgtccg ccggctcccc aaccgcacga cgtgaatcgc
    22621 catcactgtc tccccaagcc cgcgcgaccc tgccggcagc gccttcggcg cttcgacggc
    22681 cgcacgcggc attcaacgat cctaaccggg cgaacggagg acggaagacg gaaagggaac
    22741 cgggccggcg ggaggctccg gttgagcagg ccccggcgaa ttggtaaggt aagcgggtgc
    22801 tgtccgagga cattttcaag atcctgctgg caatcggcat cggaggcgcc atcggtctcg
    22861 agcgcgaata ccgagacaag gcggcaggct tccgcacgct gatcttcatc tgcgtcggcg
    22921 cgacgctgtt cgcgatgctg tcgagcaagc ttgcgggcga ccgcgatccc acccgcatcg
    22981 cggccaacat cgtgagcggc gtcggctttc tgggggcggg cgtcatcctg cgcgacggcg
    23041 ggcgcgtggt gggcttgacc accgcggcca cgatctggct ggtggcggcg ctggggacag
    23101 ggttggccgg cgggtactat gcgctcgtcc tcggggcgac ggccctcatc gaggtcgtcc
    23161 tctgggggtt cccgttcctc gagcactgga tcgaccgcat ccgcgaagag cggaactacg
    23221 aggtggtcgt cgggcgcgtc gagaagtgcc aggccgtcga ggacgtcttc cgggagtgcg
    23281 ggctgcggat cgtgcgccac cgggagagcc gggtcggccc gcggctggcg tgcacctggc
    23341 aggccaccgg cacgccgcag gcgcaccggc ggctgatcga gcggctgctg gcagacgccg
    23401 agatcgacga ggttcgcgcg tgacgtcact cgatctcctc gtcttcggcc ctcaccccga
    23461 cgacctcgag atcggtctcg gcgggacgat tgccctccac gccgccgccg ggtggcgcgt
    23521 cggtctctgc gacctcacgc gcggcgagat gggcagcaac gggacgccgg aggagcgcct
    23581 ccgcgaggcc gaggaggccc gggtggtgct tggcgcggag tggcgcgaga acctcgggtg
    23641 gcccgaccgg ggcatcaccg gcgaccgcgc gcagctcgac caggtgacgc ggctggtgcg
    23701 ggtctggcgg ccgcgggtgg tcgccatccc ttactggcac gaccgtcacc ccgatcacgt
    23761 ggccgccagc cgcgtgctca gcgaggccat cttcaacgcg aggcttcgcc gctaccagcc
    23821 ggagggcggc gggcccggtg ggtgggcccc cgagtgggtc tgctactact tcatcaacga
    23881 cgacgggccg atctccttcg ccgtcgacgt ctcggcccac tatgatgcga agcgccgggc
    23941 gctcgagtgc tatcgcagcc agttcatgcc cgaggggccg ggggcggtgg acactcgcct
    24001 cacggcgcca acctttgcgc gcctgatctc cagccgggat gcgcggttcg gcgcgctggc
    24061 cggcgtcgag tacgcggagg gggtggtggt gaaggagccc gtccttcggc ccggcctgtt
    24121 gaaggactgg acggcgcagc cggcgccgcg atcggaccgt cgatgaagat cgccatcgtc
    24181 tgctacgcct cgatcggcgg cagcggcatc gtcgcgaccg agctggccaa gtgcctggcg
    24241 gtgcgcggcc accaggtgca cgtggtgagc cgcgagcagc cgttccgcct cggcgacgac
    24301 gagccgggcg tggctttcca ccaggtgctc acgccgtcct acccgctgtt ccgcgagccg
    24361 cagtacctgc tgtcgctcgc gacgcgcatc gcgcagctct cgcgcgaggt cggcctcgac
    24421 atcatccacg cgcactacgc gatcccgcac gccacggcgg cgtacctcgc gaggcagatc
    24481 ctcgagagca cgggcggccc gacgccgaag gtggtcacga cgctccacgg gaccgacatc
    24541 accatcgtgg ggagcgatcc gtcgtacgcc gagacgatcg gcttctcgat cgatcggtcg
    24601 gacggggtca ccgcggtgtc ggagagcctc aaggccgaca cgtaccgggg cttgccgatc
    24661 acgcgcgaca tccgggtcat cccgaacttc ctcgactgcc gcatccatca ccgcgtgccg
    24721 gccaacgggc tgcgcgatcg gtttgccggg cacggccgat cggtcgtcat ccacgtctcg
    24781 aacttccgcc ccgtcaagcg gatcgacgcc gtgatcgacg tgttcgcgcg gatccgggcc
    24841 cgccggccca gcgtgctgct gctggtgggc gaggggcccg atctcccgct cgcccgggag
    24901 ctggcgcagc agcgcggcat cgaggaggat gtgctggccc tgggaggcca ggaggacgtc
    24961 cgccagttcc tctcgattgc cgacctgttc ctgctgccgt cggctaccga gagcttcggc
    25021 ctcgcggcgc tcgaggcgat ggcgtgcgac gtgccggtgg tggcgtcgcg cgtcggcggg
    25081 ctgcccgagg tcatcgagca cggcgtgagc gggtacctgc acccgcccga ggaggtgggg
    25141 gcgatggccg aaagcgggct ggcaatcctc accgaccccg gcctgcacgc gcgcgtcgcg
    25201 gccgcggccc ggcgggtcgt ccacgaccgc ttctgcgccg agaagatcgt gccgcgctac
    25261 gagcgcttct acgaggaggt gctggcgggg tgacgccccg ccaggcacgt cagcatgggg
    25321 gcgccatgac ctccctcgaa cgcctgcaga acgtcctcgg cgcgcgcccc gtggaccgtg
    25381 ttccgaactt cgacatcttc atgacgaggg cggcgcacca catcggcgcc ccgctgtccc
    25441 ggtactacct cgatcaccgg gtcctcgccg ccgccaacct cgccgttctc gacgacttcg
    25501 gcctcgacat cgtgcaggcg atctccgatc cgtaccgcga ggctgccgac accggcctcg
    25561 aggtgacgtt ccccgacgac ggcctgccgg tgagcacggc gccgctgatc gtcgaacaca
    25621 gcgatctcgc ccgcgtgcgg tttccggtcg ccgggttcgg cccgcggatg accgaccggc
    25681 tcgaggcgat ccgcgtgatg cgagccgagg tcggtgacga ggtgccggtg atgggatggg
    25741 tcgagggcgc gcttgccgag gcggccgacc tgcgcggcat gagccaggtg atgctcgacc
    25801 tcgtgatgtc gccgggctgg ctcgaggagc tgctggaacg gtgcgtggag gtcggggttg
    25861 ccttcgccga ggcacaggtc gaggccggcg cccacatcgt cggcctgggc gacgccgttg
    25921 cctcgcagat ctccccggcg atgtaccgcg agttcgcgct gccctacgag cgccgtgtct
    25981 tcgaggcgat caaggcgaaa gggggcatcg gccgcctgca catctgcggg aacacgacgc
    26041 acttgctcgg cgacatggcc ggcagcggcg ccgcgatcgt ggacgtcgac tggatggtgg
    26101 atctcgggcg cgcggtggac acgatgcgcg acgtggtcgt gtgcgggaac gtagaccccg
    26161 tggcggtgtt cctgcagggg acgcccgccc aggtgcgcga ggcggtgcgg gcgaacgcgg
    26221 cggcggtggg cggcgcgcgc tgggtctcgg ccgccggctg cgagatcccc gacgagacgc
    26281 cggcggagaa cctgctggcg cagcgcgagg cgctggcgtg acgctcttca ccatcggcca
    26341 ctcgacgcgg ccgatcgacg agttcctcgg gctgcttcgg ggacatggcg tgcggcggct
    26401 cgtggacgtc cggaccgtgc cgcgatcccg ccacaacccg cagttcgagg ccggagccct
    26461 ggcggccgcg ctcgagaagg ccggcatcgc ctacctccac cttcccggtc tcggcgggct
    26521 tcgccacccg cgccccgact caatcaacac cggctggcgc aatcgcagct ttcgcgggta
    26581 cgccgaccac atgcagtcgg ccgtctttgc ccagagcctg cagcgttgca tcgaccttgc
    26641 cgtggaaggg cccctcgcgc tcatgtgcgc cgaagcggtc ccctggcgct gtcaccggtc
    26701 gctgattgcc gatgcgctgc tggcaagggg gattgaagtc cgggagatcg tgagcccgac
    26761 ctctgcacgt cctcacaccc tcaccccgtt cgcgaaggta agcgggacga ccgtcaccta
    26821 cccgggggcc gtaccaaaag cgtgaagaac ctcacgcccg gccggggccg taccccagac
    26881 gtgaataacc tcacgtctct ggtacggccc cacggccccg tcctcggccc cacggccccg
    26941 tcctcggccc cacggccccg ccctaccggc gctgcttgcc cacggtgtcg cgccagttcc
    27001 cgatccaggg ctccgggaac gtccggttga agtcgatctc ggcgacggcg gcgccggcgg
    27061 ccgtatcgtg tggcacagac gccagcacgt ttccctgcgg gtccacgatc tcgctgtcgt
    27121 agtcgtaccc cgccgtcgcc agccagaccc cgttctcgat cgcgcgcgcg cggacgagcg
    27181 gcgttcgccc gccccagatc ggcacgagga tcagctcggc gcccgcgcgc gacaactcga
    27241 cggccggctc ggggaacgag gtgtcgtggc agatcatcag gcccaccctg ccaaagtcgg
    27301 cctggaacac cgggaaggcg tagcccggag cctcgccggc cgcgacttcc tcctgcggca
    27361 gctgcacctt tcgataggtg ccggccactc tcccgcggcg gtcaatcagc acgcctgtgt
    27421 tgaagatgtc aggcccggag cgctcgagca ggctgaacgc gacataggtg acgacgaagg
    27481 cgctgagcac cgggatcagc tgccgcaccg cggcgttcct gcgggtcagc agctccaaca
    27541 gtcccacgaa cagcccgaaa acggtggcgg cgaccagcga caccacggtc ggttgcagga
    27601 tcaatccgta tgcggcgctt tgcaatccgt agccgatgac gccgacccaa ccgggaaagc
    27661 ggcggggcag cgcgtgcatg cggtccagct cggccagtcc ctggaccggg tc
//
LOCUS       DAOPQX010000008        26490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2357_length_26490_cov_5.403594, whole genome shotgun sequence.
ACCESSION   DAOPQX010000008 DAOPQX010000000
VERSION     DAOPQX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 26490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26490
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_26490_cov_5.403594"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKK95_00975"
     CDS             <1..782
                     /locus_tag="PKK95_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC16804.1"
                     /translation="RYVMELAGLESKLDERTRLLILCSPHNPVGRVWTAAELEALGEV
                     CERRDLLVLADEIHMDLVLRGHRHVPFASLSEKLAARTITCIAPSKTFNVAGLATSLV
                     VASNPKLLSRYERQLRASGLGIGSLFGNVGLEAAYRHGAEWLDELLEYLEGNVNLVER
                     FLAERLPMLRFVRPEGTYLALIDCRALGMDQRALDDFFLRSARVYFDSGSMFGRETEG
                     FERINLGCPRATLLEALERIARAIESLQDRPGDPAPAAGRG"
     gene            803..1366
                     /locus_tag="PKK95_00980"
     CDS             803..1366
                     /locus_tag="PKK95_00980"
                     /inference="COORDINATES: protein motif:HMM:NF040142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16805.1"
                     /translation="MGSTGRWLRFWGVAAAYGVTFLLLRRADPGIATSPWFVVAAMIC
                     FLGLMSVAKEVVRIRMPRALRGIRDWEARGRAYRALGVAAFGALLRRTPLRLLNRDVY
                     LDRRTRHSGELSAQLEAAEASHFWAALLVIPYMVRLGLAGTWGALFWVTVAQVLANLY
                     PIMHLRMARHRLERLSARAARTPVSRP"
     gene            complement(1353..3842)
                     /locus_tag="PKK95_00985"
     CDS             complement(1353..3842)
                     /locus_tag="PKK95_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HOC16806.1"
                     /translation="MFILRTIAAASLLFLLAGQAAPPDPGLRLWYDKPAVAFEESLPL
                     GNGRIGASVFGGVATERVMLNESTLWAGGPVNPEVNPDARAWLPKVREALFKGDYKLA
                     DQLTRKLQGKFSASYAPLGDLHIETPEARIAGAAGFRRQLDLDAATARTSFTVSGTSF
                     EREYFVSHPDRILVMRLTASSPGALEFAVRASSQLRHEARIAGDGDLLLTGRAPVRAE
                     PNYRGNMKDPIAYDESPAPKGTRFAARVRVLGTDGKVERREGRLVVTGASEALLAVAV
                     ETSFVAFDREPSLGPDPSRTTAARLDAVRGRSFESLRDAHLADHRALFRRVSLDLSPA
                     GGSVGRASSPAPASVAVGQASSPVASALPTDERLRQYAAGAPDPGLEALYFQFGRYLM
                     IAGSRPGGPPLNLQGIWNPHVRPPWSSNYTININTEMNYWPAETTNLAECHQPLLRFL
                     SGLAATGAVTARHFYGCGGWCAHHNSDVWALSNPVGDLGQGSPVWANWPMGGAWLSLH
                     LWEHYAFNRDTNWLRATGYPLMKGAAEFLMDWLVEGPDGFLVTAPSTSPENQYKTPDG
                     YVGEVSVMTTADLALTRGLLLKVEQAGSILGDDPAFRDRVRKTLDRLPPYKVGRLGNL
                     QEWYQDWDDQDPQHRHVSHLIGLFPDNQITTFDTPELAAAVKRSLELRGDGGTGWSKA
                     WKIALWARLLDGDHAYRMLRTHLRYVQPTGVTQYGGGGGTYPNLFDAHPPFQIDGNFG
                     GTAGIAEMLLQSHRGEIHLLPALPSAWKTGSVRGLRARGGYTVDIAWKDGALAEAAIR
                     ADHDGTVRVRHGGTISELKIRKGETGRITGD"
     gene            4170..5606
                     /locus_tag="PKK95_00990"
     CDS             4170..5606
                     /locus_tag="PKK95_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC16807.1"
                     /translation="MDHEALYQARLARYVTAMRNEVPDRVPIRPFVAEFTARYAGMTC
                     QDVAHDYRKAFEAAARCARDFDWDAVVANMVYVWTGLTQAAGLRYYGIPGIEVPLAVG
                     FSYLEPRPDQAFMRADEYDALIADPTAFLFNTWLPRISADVAAPGTPASYRGTLALVK
                     SAMAMTQYFQAFGPQLERLRLECGMPSAIAGIFKAPFDILADKLRGYLGLVDDMARQP
                     AKVLAACEALAPHLCRVGLATADPARLVPIGFWMHRGCVPFFSPKQFASHNWPTLRPI
                     IEEFWKQGHQTLFYAEGKWAAHLEAFRELPARSIVFHCDRDDVFEVKRKIGDRFAISG
                     GVPNTLLSFGKPGEVRDFVRRVIAEAGEGGGYILDAGAIMQNDTSVENLRVLTDTARE
                     FGVYRGAPEPTATPPCDLPASVADREAVRGLAGWPKPRVGPGVCIPWEEKLKELPPIT
                     GDADLVRRVWEEVDVLGNDYIWQLLLSF"
     gene            5603..5794
                     /locus_tag="PKK95_00995"
     CDS             5603..5794
                     /locus_tag="PKK95_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16808.1"
                     /translation="MSTREIVQVVQEVQKVPRVLVLVVQGVLVLVVQGVLVLVVPKVR
                     VPGVQAQHSRHRHFRHHQH"
     gene            complement(5849..6736)
                     /locus_tag="PKK95_01000"
     CDS             complement(5849..6736)
                     /locus_tag="PKK95_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16809.1"
                     /translation="MRLSLAAPVVAAWLAAASPCLAASQGPGLPQLEWRAPEALAPQV
                     ERLARFDTGRLLAVMRLVGLEEAGPPIPVVLVPEDAPLARDTPQWIAGFATGGRAVVL
                     FPARTPSYPYDSVDQLLQHEIAHVLIARAAGGRLVPRWFHEGVALAAERAWTLGDRAQ
                     FAIDVAFGGRIAASSLDGLFEGDVATVRRAYRLSGLLVEDLLRTHGSNLPAQVLSAMR
                     GGLSFDAAFETSTGMSVDEASRAFWRRRRFWAAWLPWLTSATALWTLITLLALAAIAR
                     VAWRRKRRKWEEEEEDEIE"
     gene            6873..8276
                     /locus_tag="PKK95_01005"
     CDS             6873..8276
                     /locus_tag="PKK95_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOC16810.1"
                     /translation="MVEPIISPWLILAAGVATIVGLIIVVKANAFLALLAAALVVSVL
                     APGEAGDKAARVATAFGTAAGSIGIVIALASMVGRSMTESGAADRIVSAFLRAFGERR
                     GDVALVGSGYALSIPIFFDTVFYLLVPLARSMTRRTGRHYVRNLLAIAAGACATHTMV
                     PPTPGPLAVAANLGVDLGLLMLVGILVAGPASAVGLLVATWRDRALRIEYRPMPGVDE
                     EASAIADGQLPPLGLSLVPILLPVLLIGGHSILSTAASRGVAVPPALASAAALAGNAN
                     FALLVAAVAALALVQFTRRPTRERLASMVEESLMSAGIIILITAAGGAFGAMLRAANV
                     GAGVETLFRPGAAGASGVAVLLLAALMSSVFKVAQGSSTVAMLTTSALFGAMLPGGGL
                     AVHPVYLALAISGGSLVGTWMNDSGFWIFAKMGNLTEAETLGSWTILSASVGLTGIVL
                     TCVLAWLFPMAPVPVTP"
     gene            8273..10246
                     /locus_tag="PKK95_01010"
     CDS             8273..10246
                     /locus_tag="PKK95_01010"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR03432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HOC16811.1"
                     /translation="MSQRYFDASGPAIYDLRTSGGGPPGALPITPDALLAQPSGNLFG
                     WTQNVGMGWEPGDFGRKEFLILSTLGGLRAPDGSPVALGYHTGHWEVGLLVEAAAREV
                     KQLGGVPFAGFCTDPCDGRTQGTPGMFDSLPYRNDAATVLRRLIRSLPQRRGVVGVAS
                     CDKGHPAMMMALAGCRDLPGVLVPGGVTLPARGAEDAGTVQTLGARYAHGLVSLEQAA
                     ELSCRACGSPGGGCQFLGTAATSQVVGEALGLSVPHSALAPSGSEVWAEGARQSVRAL
                     LDLEARGLALRDILTDRSIRNAMVVHAAFGGSTNLLLHLPAVAHAAGLRRPTVEDWAR
                     VNREVPRLVSVIPNGPVLHPTVRVFLAGGVPEVMRHLRLLGLLDLDCLTALGRPLGDV
                     LDEWEVCERRARFRQVLRDQDGVDPNTVIMDPDAAHAAGLTSTVSFPRGNVAPGGSVV
                     KSTAIDPRVVGPDGVYRKRGPARVFGREADAIAAIKSREADAVRPGDVVVLAGCGPRG
                     TGMEEIYQVTSALRHLPWGHEVAVVTDARFSGLSTGACIGHVSPEALAGGPLGRLRDG
                     DLVEIVIDRVSLEGSVNLVGAGGEVRGAEWGSRELATRPANPAVGPHPQLPDDTRLWA
                     ALQEAGGGTWGGCVYDADAIVAALEKKKRTGDS"
     gene            10268..11164
                     /locus_tag="PKK95_01015"
     CDS             10268..11164
                     /locus_tag="PKK95_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOC16812.1"
                     /translation="MLVVQVVPKVLEVLVPKPAGRGASMILYRTTEGAFVEHEGRFAR
                     LDEEWDAIFNRRDLAAYLRKAAAAGGATRLPRGAAILPPIESQEVWAAGVTYERSRTA
                     RMSESKGAGGGSFYDRVYRAHRPELFFKATPHRVAGQGGVMRLRSDSAWIVPEPELTL
                     AIDSRGRIIGYTVGNDLSCRDLEGENPLYLPQAKTFSGCAALGPGILVSGRPLPRATT
                     ITLTIQRRGRMVFRGATELARMRKPLARLVAYLFRDNAFPLGCFLMTGTGIVPPDGFS
                     LRRGDTVRITIPPIGTLVNRMG"
     gene            11221..12804
                     /locus_tag="PKK95_01020"
     CDS             11221..12804
                     /locus_tag="PKK95_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HOC16813.1"
                     /translation="MTLHGCQIIAGRAVAAGPDTFLGRNPATGEATTPPVHEATSVEV
                     DQALEAADAAFDDYRRLGAERIASFLEAVASEWMALGDDLLAQAHAETALPAARLTGE
                     RARMVNQARLFAALAREGSWVDARIDHGDPSRQPVPKPDVRRMLEPIGPVAVFGASNF
                     PFAISVGGGDTVSALAAGCPVVAKAHPGHPGTSELLGRAIVSAAEKSGVPAGVFGMVQ
                     GRGHEVGLALVRHPLTRVVAFTGSQAGGRALFDAAAARPDPIPVCAEMGSTNPVFVLP
                     GAMAERGAEIAEGYVQSVTLGTGQFCTNPGVLLAVEGPALVAFLDRVAAAAAAVAPST
                     MLNEGICRAFHGGLDRVRQTHGVRVLRESHARADRARAEAGCAIFTASLSTLEARPEL
                     WDEVFGPSSIVIVCPGADDLERAARRLHGHLTASIHATAAELESNRQLVAALERHVGR
                     IVFNGFPTGIEVCAAMHHGGPWPATTDVRYTSIGTASIERFVRPVCYQGFPQPALPAE
                     LQDVNTRGIWRLVDGVRCR"
     gene            12857..15139
                     /locus_tag="PKK95_01025"
     CDS             12857..15139
                     /locus_tag="PKK95_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC16814.1"
                     /translation="MPLAPASRLGPYEVVDLLGAGAFGEVYSARDTRLGRHVAVKMLS
                     AARAGDPQQLERLKQEALTAGSLSHPNVVSVYDVGTEGESFFIVSELVEGRTLRHHLV
                     QGPLPPGLALDYAAQAARGLAAAHEKGIVHRDLKPENLMVTPDGRVKILDFGIAKVDS
                     TGARLDEAGLLPTTVEETLPGTVLGTAAYMSPEQVRGESVDGRSDLFSLGAILFEMLT
                     GRRAFDGATAAETIASILRDTPVLPSSVPPAAAAIVRRCLEKQPGRRHESARQLAEAL
                     DAVLGRSSPGVAAARLVPVAVPEEPPRVAVLPFTNLGREPEQEYFSDGLTEQIIHELT
                     RIRGVRIVAWQSSAKMKGHEENIHEAARQLGVELLLVGSVRQARDRVRIAARLVEVTT
                     GYYRWSETYDRQILDLFAVQEEIARAIAGTFERTLGGSAVRRERRAPASLEAYDLYLR
                     GRFVGSQRTKAGLIGAVECFEQAIALDEEFALAHAGLADALCLLSDYGLTAPEDAMPR
                     ARAAALRALDLDPGSGEAHASFALIHSIYDWEWEEAEAFYRRSIELNPGYATAHHWFA
                     LDFLANVGRLDEAQEELDLARQLDPLSAIIQEGKPYLLMMQRRYDEALAGFKALADLQ
                     PGFYKAYTTMGRTYSLMGRYEEAIAMLERGRELSGGEVPNILGALGQSFGLAGRPDRA
                     RELLDQLAALSREKYVPATCFALIHAGLGEHDAAVDWLERGAERRDLPLSSIKTHPAY
                     DSLREHPRFQALLARLRFPG"
     gene            complement(15148..16002)
                     /locus_tag="PKK95_01030"
     CDS             complement(15148..16002)
                     /locus_tag="PKK95_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOC16815.1"
                     /translation="MSGDEAGFSRVGIAGLGLIGGSVALAVRRAWPGAVVAGMDLDEE
                     VVRDALARGVVDRASTKLASLDDVDLIVLAAPVGANAALLPALARRAREDALLTDAGS
                     TKRDILAAARWAGAERQFVGGHPLAGAAEGGLAHASASLFDGRPWLLTPGPDTPPPVV
                     SRVEQFVGALGAVPRTLEATEHDRLMAAVSHLPQIVASALMEVAGELAGEEGLALAGK
                     GLADTTRLASSPAGVWRDVCAANADCLSLALRRLIDELEALRDDLPHGDRLAEVFQRA
                     RAWRQQLR"
     gene            complement(15999..17114)
                     /gene="aroF"
                     /locus_tag="PKK95_01035"
     CDS             complement(15999..17114)
                     /gene="aroF"
                     /locus_tag="PKK95_01035"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOC16816.1"
                     /translation="MVVVMQEGASEEQIDRVIDALVRMRLAVHRSTGVSRTVIGVVGN
                     TGAIDPASVEILDGVHEVLRVSQPYKLASRAFTPDDTVVTVGDVRIGGDEVIVAAGPC
                     AAETEEQVEATAAAVKRAGAKLLRGGAFKPRSSPYAFQGLGEEGLQMLRRAADRHGLP
                     LVSEVMDVAQIEVVSRYADILQVGARNMQNFTLLRELGLVRKPVLLKRGISATIEEWL
                     LSAEYVLAGGNRDVILCERGIRTFENATRNTLDLSAIPVVKKVSHLPVVVDPSHGTGR
                     RDKVVPMARAAVAAGADGLLVEVHCNPDQALSDGAQSLYPAQFDDLMGELRIIGPAIG
                     RRVCVKTGSGLKAQGSGPTEGSELKAQGSGLAEGSER"
     gene            complement(17108..17413)
                     /locus_tag="PKK95_01040"
     CDS             complement(17108..17413)
                     /locus_tag="PKK95_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOC16817.1"
                     /translation="MTIEDLRARIDAIDDQLVSLLNARAACAIEIGRLKRRDALPIFQ
                     GDREQAVIRHSRAVTARLGGPLGEEAIARLFERIIDEARRLEAAFEESAEREGGASW"
     gene            complement(17703..18338)
                     /locus_tag="PKK95_01045"
     CDS             complement(17703..18338)
                     /locus_tag="PKK95_01045"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOC16818.1"
                     /translation="MVKPLTGSARPPVMAKIDYKKSLPEVYGARRAPAEVRVPGGTFL
                     AVDGVGEPGDAAFQEAMHQLFTLAYTTRFTMKKAGGVDFTVPALECVYLDNPCEKGRR
                     EWHWRVMVRVPPCVKAPTLREVRKAIAAKKGPATDAVKRITWAEGKALQLLHVGPYDQ
                     VGTAYQRLADETAARGLQVVGPAHEIYLNDPRRVAPERLKTIVRMAVKKGR"
     gene            18551..21931
                     /locus_tag="PKK95_01050"
     CDS             18551..21931
                     /locus_tag="PKK95_01050"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOC16819.1"
                     /translation="MRHRTTTVLALFVTFLVAPLAGQPARLGYYRFPAIWRDTLVFTA
                     EGDLWRVPLAGGVAQRLTTHPAAETRPAISPDGRTVAYSASYEGPTEVYTLPLEGGVP
                     VRQTYDGGNAQVVGWTPAGEILYATTRFSGLPNTQLAKIDPATRTRTLVPLAQASDGA
                     YDAKATTLFFTRLAFQGSHTRRYRGGTAQNLWKFTDGAEAVPLTGDYDGTSKTPMPWQ
                     GRIYFASDRDGAMNIWSMAEAGGDLRQHTQHRDFEVRSPSLSEGRIAYQLGADIHVLD
                     LASGNDRAVPITLVSDFDQMREKWVTSPIDWVTSAHLSPDGDRVALTARGQVFVAPAL
                     QGRLVEATRNPRVRYRNARFFPDGKTVLALSDESGEVEFWRVPANGVGSPAQLTSDGK
                     VLRWDGLPSPDGRLIAHHDKDGLLWIYDIAKKTQTKVAEALDGRFDEIQWSPDSRWLA
                     YVVPGPNQLARIWVLEAATGRVTPVTTDRYDSGSPAWSPDGKWLYFLSDRHFESSVSS
                     PWGSRQPEPYFDKQTKVYALALKKGERSPFQPDDELHPAKKEEAKEPKKEQAGEEKPA
                     SAKDAKKDVPKGGKKDAAPAGKPDEAAKPAAVPRVEIDLEGIEARIVEIPVPPGNYSD
                     LSVDGKRLYYVSRDAGRDAKRMLKTFPIDNKKPEAETFAEDIVSYELSQDGKKLLLRK
                     AKDFYVLDAAAKAPAAPELAKRQVPLKDWTFRLDPREEWAQMYQEAWRLERDYFYDRG
                     MHGLDWPAIKARFQPLAERVTDREELSDVLAQMVSELSALHIFVYGGDRREGQDRVTS
                     ASLGARLARDEAAGGYRVEHIYRSDADRPDRLAPLTRPGVGVGEGDVIEAVNGVPTLS
                     VPDIASLLRNQADKQVLLRVKPKGGGAARDAIAVPISQSRENELRYDEWEYTRRLAVE
                     KASGGKIGYVHLRAMGSNDIAQWYREYYPVFDREGLIIDVRHNNGGNIDSWVLGRLLR
                     KAWFYWQPRVGRPTWNMQYAFRGHLVVLCDQSTASDGEAFAEGFRRLGLGKLIGTRTW
                     GGEIWLSSSNVLVDRGIATAAEIGVYGPEGQWLIEGHGVDPDIVVDNLPHATFKGEDA
                     QLDAAVQHLTELIKTKPVQVPPPPPYPIKRGGENRGKM"
     gene            complement(21935..22762)
                     /locus_tag="PKK95_01055"
     CDS             complement(21935..22762)
                     /locus_tag="PKK95_01055"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOC16820.1"
                     /translation="MKPLHTLAIACAAVSLAAALAAQAPGKKPEKKKVLCIGESQGWQ
                     HDAVTYGLATIWRWGHETGLFDTYIRTDCELITKKKPGNDNAKNLDWFDAVVFYTTGE
                     LEMDESQKADLISFVKDDGKGFVGVHSALDTFYKWPEYGEMVGGYFDGHPWNTFDAPI
                     LIEDPGSPMTRHFTSRSIVVNDEIYQAKDWSRDKVRVLMRMDESKLDLTKKGIKRADK
                     DWAVTWIKSYGKGRVFYSTLGHTYEAWDDKGVATMYLEGIRWALGLTEADTRSLPKK"
     gene            complement(22846..23577)
                     /locus_tag="PKK95_01060"
     CDS             complement(22846..23577)
                     /locus_tag="PKK95_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOC16821.1"
                     /translation="MSDSFLLSSSVAFGDVDRDNLLTLQGAFKLLQEAAIAHANLYDT
                     GTDAMLTRGESWVLKRMAVAIDRYPRYGEALRVETWSTGIRGSVGNRDFRVDAADGQT
                     VMRASSLWLYISVRAKAVVKVPPEIADRFPSRSGDVFCPELQALAFDAPDSKAAASLP
                     VSLRYSDVDANDHVNNTVYLDLLQTALSRLGCPPRPGSVRIKYGGGIPGSTAAVEVRL
                     APAGGGGRIFSIASGDEVFAVGSCA"
     gene            23919..24743
                     /locus_tag="PKK95_01065"
     CDS             23919..24743
                     /locus_tag="PKK95_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOC16822.1"
                     /translation="MRKLLLVTVALVLVSWPSLALAQATPVPAPAAAAAQAPAGWTLV
                     FADEFDKPGAPDPAKWDYELGYIRNKEAQFYSSRPENVRVEGGSLVIEGRKEPYEGFA
                     YTSASINTRGRFEFLYGRVEVRAKLPAGRGAWPAIWTLGTNRGKVRWPACGEIDIMEN
                     VGFAPLRIHASVHTAAYNHVAGTQKTAIVEIDDPSKDFHVYAMEWRAGRIDVFVDGRK
                     YFTFENEGTGNDAWPFDKPQYLLINLAIGGMWGGQEGIDDSSFPQRYLVDYVRIYR"
     gene            24755..26260
                     /locus_tag="PKK95_01070"
     CDS             24755..26260
                     /locus_tag="PKK95_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC16823.1"
                     /translation="MNRRQALARLAAIPAAARLAGAAAHAAPTNRNVLFILTDDHRYD
                     AMGFMKPQPFLETPNLDRLAREGAHFQNAFVTTALCSPSRASILTGMYAHRHKIVDNN
                     TAIPPGTEFFPQRLQQAGYKTAFIGKWHMGNSGDQPQPGFDHWVSFQGQGSYRPVPSG
                     LNVDGKKVPQKGYITDELTDYAVEWLRSLPATQPWFMYVSHKAVHSEFVPAPRHAGRY
                     RNATFVPPKTMAATGDMARGRPMWVQNQRNSWHGVEFPYHSDLDIGEYYKQYAETLLA
                     VDDSVGRLLDELEKRGQLDSTLVVFMGDNGFAFGEHGLIDKRTAYEESMRVPLLARCP
                     ELFGGGRVVTPMVAGLDIMPTVLDACGAAIPGGIDGRSWLPLVRGNAEGWRTELLYEY
                     YWERNFPQTPTVHALRTDRYKFTRCHGLWDIDELYDLADDPLESNNLIFSPAHQPTVR
                     TMRARLFQLLEETGGMSIPLYPDMGFQGNLRHPDRSPAAEFPPELAPRRKR"
BASE COUNT         3866 a         9287 c         9455 g         3882 t
ORIGIN      
        1 ggcggtacgt catggaactc gccggcctcg aatcgaagct cgacgagcgg acgcgcctcc
       61 tcatcctgtg cagcccccac aacccggtgg gccgggtctg gaccgccgcc gagctggagg
      121 cgctgggcga ggtctgcgag cgccgcgacc tgctggtgct ggccgacgag atccacatgg
      181 acctggtgct gcgcgggcac cggcacgtgc cgttcgcttc gctctcggag aagctggcgg
      241 cccggacgat cacctgcatc gcgccgagca agacgttcaa cgtcgccggc ctcgccacct
      301 cgctggtggt cgcgagcaac cccaagctgc tttcgcgcta cgaacgccag ctgcgcgcgt
      361 cggggctcgg catcggcagc ctgttcggca acgtcgggct cgaagccgca tacaggcacg
      421 gggccgagtg gctggacgag ctgctggagt acctcgaggg gaacgtgaac ctggtggagc
      481 ggttcctggc ggagaggttg ccgatgctgc gcttcgtccg gcccgagggc acctacctcg
      541 ccctgatcga ctgccgggcc ctcgggatgg accagcgcgc gctcgacgac ttcttccttc
      601 ggtcggcgcg ggtgtatttc gacagcggat cgatgttcgg ccgggagacg gagggcttcg
      661 agcgaatcaa cctcggctgt ccgcgggcga cgctgctcga ggccctggag cggatagcgc
      721 gggcgatcga atcgctccag gaccggccgg gggatcctgc gccggctgca gggcgcggat
      781 gaagccggcg aagaagcggc ggatgggctc aaccggaagg tggctgcggt tctggggcgt
      841 ggctgccgcg tatggcgtga cgttcctgct gctgcggcgg gccgatcccg gcatcgcgac
      901 gtcgccctgg ttcgtcgtcg cggcgatgat ctgcttcctt ggcctgatga gcgtggcgaa
      961 ggaggtggtg cgcatccgga tgccgcgcgc gctcaggggc atccgcgact gggaggcgcg
     1021 gggccgcgcc taccgggcgc tgggcgtcgc ggcgttcggc gcgctgctgc gacgcacgcc
     1081 cctgcggctg ctgaacaggg acgtgtacct ggatcgccgg accaggcaca gcggagagct
     1141 gagcgcgcag ctcgaggccg ccgaggcctc gcacttctgg gccgcgctcc tggtgatccc
     1201 ctacatggtg cggctcggtt tagcgggcac ctggggtgcg ctgttctggg tgaccgttgc
     1261 gcaggtgctc gcgaacctgt atccgatcat gcacctccgg atggcgcgcc accgcctcga
     1321 aaggctgtcg gcgagggccg cgagaacgcc cgttagtcgc ccgtgattcg ccccgtttcc
     1381 cccttcctga tcttcagttc ggagatcgtc ccgccgtgcc gcacgcgcac ggtgccgtcg
     1441 tggtcggcgc ggatggcggc ctcggcgagg gccccgtcct tccacgcgat gtccaccgtg
     1501 tagccgccgc gggcgcgcag gccgcgcacc gagccggtct tccaggccga ggggagcgcg
     1561 ggcagcaggt gaatctcgcc gcggtggctc tggagcagca tctcggcgat gccggcggtg
     1621 ccgccgaagt tgccgtcgat ctggaacggc gggtgggcgt cgaagaggtt cgggtacgtg
     1681 ccgccgccgc cgccgtactg cgtgacgccg gtcggctgca cgtaacgcag gtgtgtgcgc
     1741 agcatgcgat aggcgtgatc cccgtcgagc aggcgcgccc agagcgcaat cttccacgcc
     1801 ttgctccagc cggtgccgcc gtcgccccgc agctcgagcg agcgcttcac ggcagccgcc
     1861 agctcgggcg tgtcgaaggt ggtgatctgg ttgtcgggga agaggccgat caggtgcgag
     1921 acgtggcggt gctgcgggtc ctggtcgtcc cagtcctggt accactcctg gaggttgccg
     1981 aggcgcccca ccttgtaggg gggcaggcgg tcgagcgtct tccgcacgcg gtcgcggaac
     2041 gccgggtcgt cgccgaggat ggaaccggcc tgttcaacct tcagcagcag ccctcgcgtc
     2101 agcgcgagat cggcggtcgt catcaccgac acttcgccga cgtagccgtc aggcgtcttg
     2161 tactggttct cgggcgaggt ggacggggcc gtcaccagga agccgtcggg cccctcgacg
     2221 agccagtcca tcaggaactc ggccgcaccc ttcatgagcg ggtagccggt ggcgcgcagc
     2281 cagttggtgt cgcggttgaa ggcgtagtgc tcccacaggt ggaggctgag ccacgccccg
     2341 cccatcggcc agttcgccca gaccgggctg ccctggccga ggtcgccgac ggggttgctg
     2401 agggcccaga cgtcgctgtt gtggtgggcg caccagccgc cgcagccata gaagtgtcgc
     2461 gccgtgaccg cgcccgtggc agccaggccg gagaggaacc gcagcagcgg ctggtggcac
     2521 tcggccaggt tggtggtctc ggccggccag tagttcatct ccgtgttgat gttgatcgtg
     2581 tagttgctgc tccacggcgg gcgaacgtgc gggttccaga tgccctgcag gttcagcggc
     2641 gggccgccgg gacgcgagcc ggcaatcatc aggtagcgcc cgaactggaa gtacagcgcc
     2701 tcgagaccgg gatccggagc gccggcggcg tactggcgca gccgttcgtc agtgggcagg
     2761 gccgacgcga caggcgagga cgcctgtccc actgcgaccg atgccggggc aggcgaggac
     2821 gcccgtccca ctgagcctcc cgcaggcgac aggtcgagcg agacgcggcg gaacagcgcg
     2881 cggtggtcgg caaggtgcgc gtcgcgcagg ctctcgaacg agcggccgcg gacagcgtcc
     2941 aggcgggcgg ccgtggtgcg cgacgggtcg gggccgagcg agggttcgcg gtcgaaggcg
     3001 acgaagctcg tctcgacggc cacggccagc agcgcctcgc tcgcgccggt caccaccagc
     3061 cggccctcgc ggcgttcaac cttgccatcg gtgccgagca cgcgaacccg ggcggcgaac
     3121 ctcgtgccct tcggggccgg agactcgtcg tacgcgatgg ggtctttcat gttgccgcgg
     3181 tagttcggct cggcgcggac gggtgcgcgc cccgtcagca ggaggtcgcc gtccccggcg
     3241 atgcgcgcct cgtgacgcag ctggctcgag gcgcgcacgg cgaactcgag ggcgccgggc
     3301 gacgaggcgg tgagacgcat caccaggatg cgatcgggat gcgagacgaa gtactcgcgc
     3361 tcgaaggacg tccccgacac ggtgaacgac gtccgggcgg tggcggcgtc gagatcgagc
     3421 tggcggcgga atccggcagc cccggcgatg cgcgcctcgg gcgtctcgat gtgaaggtcg
     3481 ccgagcggcg cgtacgacgc cgagaacttc ccctgcagct tgcgggtcag ctggtcggcc
     3541 agcttgtagt cgcccttgaa cagcgcctcg cgcaccttcg gcagccaggc cctcgcatcg
     3601 ggattgacct cggggttgac gggtccgccc gcccacaacg tggactcgtt cagcatcacc
     3661 cgctcggtcg caacgccgcc gaacaccgac gcgccaatcc gcccgttccc gagcggcagc
     3721 gactcttcga aggcgacggc aggcttgtcg taccagagcc gcagcccggg gtcgggcggc
     3781 gccgcctggc ccgcaaggag gaagagaagg ctcgccgcgg caatcgtgcg caggatgaac
     3841 atggcaagag gatagccgaa ctctctacgc tcgagcgcca ctgggagccg caatcggcct
     3901 cctcgactcg ccggggatgt ctggtcgtgc ggcggatggc tacgtcacgt ccgtggcgcc
     3961 tgcggccagc gggtcctgtc gccggtcacc cccgtcgcgt cctcgcgcga aaaacactcc
     4021 aacgggggac gcgctccggg cgcgccgggg gtcccccgac cggcgccacc cgctggtcga
     4081 ggcccggaca ggacccgccc cgacgacctt gcgagtcact gcggcgggcg caggggaaat
     4141 gggcttcggt gtatgctcct gttgccgaca tggaccacga agctctctac caggcgcgcc
     4201 tcgcccgcta cgtcaccgcg atgcgcaacg aagtgcccga ccgcgtcccc atccgcccgt
     4261 tcgtggccga gttcaccgcc cgctacgcgg gcatgacgtg ccaggacgtg gcacacgact
     4321 accggaaggc cttcgaggcc gccgcgcgct gcgcgcgcga cttcgactgg gacgcggtgg
     4381 tcgcgaacat ggtgtacgtc tggaccggcc tcacccaggc ggcggggctg cgctactacg
     4441 ggatcccggg gatcgaggtg ccgctcgcgg tcggcttcag ctacctcgag ccgaggcccg
     4501 accaggcctt catgcgcgcc gacgagtacg acgcgctcat cgccgacccg accgcgttcc
     4561 tcttcaacac ctggctgccg cgaatctcgg ccgacgtggc cgcccccggc acgcccgcca
     4621 gctaccgcgg caccctcgcc ctggtgaaga gcgcgatggc gatgacgcag tacttccagg
     4681 ccttcggccc gcagctcgag cggctgcgcc tcgagtgcgg catgccctcg gcgatcgccg
     4741 gcatcttcaa ggcgcccttc gacatcctcg ccgacaagct gcgcggctac cttggcctgg
     4801 tggacgacat ggcgaggcag ccggcgaagg tgctcgcggc ctgcgaggcg ctcgcgcccc
     4861 acctgtgccg ggtcggcctc gccaccgccg accccgcgcg cctcgtgccg atcggcttct
     4921 ggatgcaccg cggctgcgtg ccgttcttct cgccgaagca gttcgcctcg cacaactggc
     4981 cgacgctgcg gccgatcatc gaggagttct ggaagcaggg ccaccagacg ctcttctacg
     5041 ccgagggcaa gtgggctgca cacctcgagg cgttccgcga actgcccgcc cgcagcatcg
     5101 tcttccactg cgaccgtgac gatgtgttcg aggtgaagcg gaagatcggc gaccggttcg
     5161 cgatcagcgg cggcgtgccg aacacccttc tcagcttcgg caagccgggc gaggtccgcg
     5221 acttcgtccg gcgcgtgatc gcggaggcgg gggagggggg cggctacatc ctcgacgccg
     5281 gcgccatcat gcagaacgac acgagcgtcg agaacctgcg ggtgctgacc gacaccgccc
     5341 gcgagttcgg ggtttaccgc ggcgcacccg agcccaccgc cacgccgccg tgcgaccttc
     5401 ccgcctcggt cgccgaccgc gaggccgtgc gcgggctggc gggctggccc aagccgcgcg
     5461 tcggtcccgg cgtctgcatc ccgtgggagg agaagctgaa agaactgccc ccgatcaccg
     5521 gggacgccga cctcgtgcgc cgggtgtggg aggaagtgga cgtgctcggg aacgactaca
     5581 tctggcagct gttactgagt ttttgagtac ccgcgagatc gtgcaggtgg tgcaggaagt
     5641 gcagaaggtg ccgcgggtgc tggtgctggt ggtgcagggg gtgctggtgc tggtggtgca
     5701 gggggtgcta gtgctggtgg tgccaaaggt gcgggtgccg ggggtgcagg cgcagcactc
     5761 tcggcaccgg cacttccggc accatcagca ctagcacccc ctgcactatc tgcacctcct
     5821 gcaccccctg cacgatctga tcccacgctc actcaatctc gtcttcctct tcttcttccc
     5881 atttccgtcg cttgcggcgc cacgcgaccc tggcgatggc cgcgagcgcc agcagcgtga
     5941 tcagcgtcca gagcgccgtc gccgacgtca gccagggcag ccacgcggcc cagaagcggc
     6001 gccggcgcca gaacgcgcgg ctcgcctcgt ctacgctcat accggtcgag gtttcgaacg
     6061 ccgcgtcgaa ggaaaggccg ccccgcatgg ccgacagcac ctgcgccggc aggttggagc
     6121 cgtgcgtccg gagcaggtcc tcgacgagca ggccgctcag gcggtaagcg cgccgcacgg
     6181 tggcgacgtc tccttcgaac agcccatcga gcgaggaggc cgcgattctg ccgccgaagg
     6241 cgacgtcgat cgcgaactgc gcccggtcgc cgagcgtcca ggcacgctcg gccgcgagcg
     6301 cgaccccctc gtggaaccac ctcgggacaa gtcgtcctcc cgcggcgcgg gcgatcagga
     6361 cgtgcgcgat ctcgtgctgc agcagctggt cgaccgagtc gtaggggtag gacggcgtgc
     6421 gggccgggaa cagcacgacc gcgcggcctc cggtggcgaa gccggcgatc cattgcggcg
     6481 tgtctcgcgc gagcggggca tcctcgggca cgaggacgac ggggattggc gggccggcct
     6541 cttcgagccc caccagccgc atgacggcca gcaggcggcc ggtgtcgaac cgggcaagcc
     6601 gctcgacctg aggcgcgagc gcctccggag cgcgccattc gagctgcggc agccccggcc
     6661 cctgcgaggc cgcgaggcag gggctggccg ctgccagcca ggctgccacg accggtgcag
     6721 ccagcgaaag gcgcatcaac tgatggtaca cgccgccggt cgagagggga cgaaggtacg
     6781 cctgcttgcc cgccgtagcc gccggccggt ttcaagccgg cgcgatggcg ggagccttga
     6841 gactgccctt ctccctgtgc catagtccgc gcgtggtgga gcccatcatc agcccgtggc
     6901 tgatccttgc cgccggtgtg gcgacgatcg tcggcctgat catcgtcgtc aaggcgaacg
     6961 ccttcctggc cctgctcgcc gccgcgctgg tggtgagcgt cctcgcaccc ggggaggcgg
     7021 gcgacaaggc cgcgcgggtt gccaccgcgt tcggcaccgc cgccggcagc atcggcatcg
     7081 tgatcgcgct cgcgtcgatg gtgggccggt cgatgaccga gagcggcgcg gccgatcgga
     7141 tcgtgtcggc cttcctgcgc gccttcggcg agcggcgggg cgacgtggcg ctggtgggca
     7201 gcggctacgc cctcagcatc ccgatcttct tcgacacggt cttctacctg ctggtgccgc
     7261 tcgcccgctc gatgacccgg cgcaccgggc gccactacgt gcgcaacctg ctggccatcg
     7321 ccgctggcgc ctgcgcgacc cacaccatgg tgccgccgac gccgggcccg ctggcggtgg
     7381 cggcgaacct cggcgtggac ctcggcctgc tgatgctcgt ggggatcctg gtggccgggc
     7441 ccgcctcggc cgtcgggctg ctggtggcaa cctggcgcga ccgggccctg cggatcgagt
     7501 accgcccgat gccgggcgtc gatgaggagg cctcggcaat cgccgacggc cagcttcccc
     7561 cgctcgggct gtcgctcgtc ccgatcctcc tcccggtgct cctcatcggg gggcacagta
     7621 tcctctccac ggcagcatcg cgcggcgtcg cggttccgcc ggcgctggcg agcgcggcgg
     7681 cccttgccgg caatgcgaac ttcgcgctgc tcgtcgccgc cgtggcggcg ctcgcgctcg
     7741 tgcagttcac gcggcgcccg acgcgcgagc gcctggcctc gatggtcgaa gagtcgttga
     7801 tgagcgcggg catcatcatc ctcatcaccg cggccggcgg ggcattcggc gcgatgctgc
     7861 gcgcggccaa cgtcggggcg ggcgtcgaga cgctcttccg gcccggtgcg gccggagcga
     7921 gcggtgtggc cgtgctgctg ctcgccgcgc tcatgtcgtc ggtcttcaag gtggcgcagg
     7981 ggtcgagcac cgtcgccatg ctcaccacct ccgcactctt cggcgcgatg ctgccgggcg
     8041 gcgggctcgc ggttcaccct gtctacctgg cgctcgcgat atccggcgga tcgctggtcg
     8101 gcacgtggat gaacgacagc ggcttctgga tctttgccaa gatgggcaac ctgaccgagg
     8161 ccgagacgct cggcagctgg accatccttt ccgcctcggt cggcttgacc ggcatcgtgc
     8221 tgacgtgcgt gctggcctgg ctgttcccga tggcgccggt cccggtgacg ccatgagcca
     8281 gcgctacttc gacgcttcag ggccggcgat ctacgacctg cggactagcg gaggggggcc
     8341 gcccggcgcg ctgcctatca cgcccgacgc gctgctcgcc cagccgagcg ggaacctgtt
     8401 cgggtggacg cagaacgtcg gcatggggtg ggagccgggg gacttcggcc gcaaggagtt
     8461 cctgatcctc agcacgctcg gcggcctccg cgcccccgat ggcagcccgg tcgctctcgg
     8521 ctatcacacc gggcactggg aggtggggct gctggtggaa gcggcggcgc gcgaggtgaa
     8581 gcagctcggc ggcgtgccgt tcgccggctt ctgcaccgac ccgtgcgacg ggcggacgca
     8641 gggcacgccg gggatgttcg acagcctgcc gtaccgcaac gacgcggcga cggtgctccg
     8701 caggctcatc cggtcgctgc cgcagcggcg gggagtggtg ggcgtcgcca gctgcgacaa
     8761 ggggcacccg gcgatgatga tggcgctggc cggctgccgc gacctgccgg gcgtgctggt
     8821 gcccggcggc gtgacgctgc ccgcgcgggg cgcggaggac gcgggcaccg tgcaaacgct
     8881 tggtgcgcgc tacgcccacg gtctcgtgtc gctcgagcag gcggccgagc tgtcgtgccg
     8941 cgcgtgcggc tcgcccgggg gcggctgcca gttcctcggg acggccgcca cgtcgcaggt
     9001 ggtgggcgag gcgctcggcc tgtcggtgcc gcactcggcg ctggcgccat cgggctcaga
     9061 ggtgtgggcc gagggggcgc ggcaatcggt gcgcgcgctg ctcgacctcg aggcccgggg
     9121 ccttgccctc cgcgacatcc tgaccgaccg ctcgatccgc aacgcgatgg tcgtgcacgc
     9181 cgccttcggc ggctcgacca acctgctgct tcacctgccg gccgttgcgc acgccgcggg
     9241 cctgcgccgg ccgaccgtgg aagactgggc gcgcgtgaac cgcgaggtgc cgcgcctggt
     9301 cagcgtgatt ccgaacggtc ccgtgctcca cccgaccgtg cgcgtctttc tcgccggcgg
     9361 ggttccagag gtgatgcggc acctgcgtct cctgggcctg ctcgacctcg actgcctgac
     9421 ggcgctcggc cggcctctcg gcgacgtgct cgacgagtgg gaagtgtgcg agcgccgcgc
     9481 ccgcttccgc caggtgctgc gcgaccagga cggcgtggac ccgaacaccg tgatcatgga
     9541 ccccgacgcc gcgcacgccg cgggtctcac gagcaccgtg tcgttcccgc gcggcaacgt
     9601 ggcgcccggc ggatcggtgg tgaagagcac cgcgatcgac ccgcgcgtgg tcggccccga
     9661 cggcgtgtac cgcaagcgcg ggccggcgcg ggtgttcggg cgcgaggccg acgccatcgc
     9721 ggcgatcaag tcgcgggagg cggatgcggt gcggccgggc gacgtggtgg tgctcgccgg
     9781 gtgcgggccg cgcggcacgg ggatggagga gatctaccag gtgacctcgg cgctcaggca
     9841 cctgccgtgg ggccacgagg tggcggtggt caccgacgcg cgcttctcgg ggctctccac
     9901 cggcgcgtgc atcggccacg tctcacccga ggcgctggca ggcggcccgc tgggccggct
     9961 gcgcgacggc gacctggtgg agatcgtcat cgatcgcgtc tcgctcgagg ggagtgtcaa
    10021 tctggtcgga gcgggcggcg aggtgcgcgg ggcggaatgg ggctcgcgcg agctcgcgac
    10081 gcggccggcc aacccggccg tcgggccgca cccgcagctg cccgacgaca cgcgcctgtg
    10141 ggcggcgctc caggaagcag gcggcggcac gtggggcggc tgcgtctacg acgccgacgc
    10201 gatcgtcgcg gcgctggaga agaagaagcg gacaggggac agctgacaag gaacagctga
    10261 cagcgtcgtg ctggtggtgc aggtggtgcc gaaggtgctg gaggtgctgg tgccgaagcc
    10321 ggccggccga ggagcttcga tgattctcta ccgaaccacc gagggagcgt tcgtcgagca
    10381 cgaggggcgg ttcgcgcgcc tggacgagga gtgggacgcg atcttcaacc gccgcgacct
    10441 ggctgcctac ctgaggaagg ccgccgcggc cggcggcgcg acgcgcctgc ctcgcggcgc
    10501 cgcgatcctc ccgccgatcg agtcgcagga ggtgtgggcg gccggggtca cctacgagcg
    10561 cagccgcacc gcgcgcatgt ccgagtcgaa gggggcgggc ggcggcagct tctacgaccg
    10621 cgtctaccgc gcccatcgcc ccgagctgtt cttcaaggcc accccgcacc gcgtggccgg
    10681 acagggcggc gtcatgcgcc tcaggagcga ctcggcctgg atcgtccccg agcccgagct
    10741 cacgctcgcg atcgacagcc gcggccgcat catcgggtac acggtgggca acgatctcag
    10801 ctgccgtgac ctggaggggg agaacccgct ctacctgccg caggcgaaga ccttcagcgg
    10861 ctgcgccgcg ctcgggcccg gcatccttgt ctccggccgc ccgctgccga gggcgaccac
    10921 catcacgctg acgattcagc gccggggccg gatggtgttc aggggcgcga ccgagcttgc
    10981 gcgcatgcgg aagccgctgg cgcggctggt ggcgtacctg ttccgggaca acgcctttcc
    11041 actgggctgc ttcctgatga ccggcaccgg gatcgtcccc ccggacgggt tttcactgcg
    11101 ccgcggcgac accgtgcgca tcaccatccc gccaatcggc acgctggtga atcggatggg
    11161 ctgatgaagc cgccgacagt ggtgggccga agtgcgctaa cggagcctgc gaggatttcg
    11221 atgactctcc acggatgcca gatcatcgcg ggccgggcgg tcgccgccgg gcccgatacc
    11281 ttcctgggtc gcaatcccgc aactggcgag gcgaccactc ccccggttca cgaggctacc
    11341 agcgtggagg tggaccaggc gctggaggcg gccgacgccg ccttcgacga ctaccgccgc
    11401 ctcggcgccg agcgcatcgc ctccttcctc gaggccgtcg ccagcgagtg gatggcgctc
    11461 ggagacgact tgctggcgca ggcccacgcc gagaccgcgc tgcccgcggc gcggctcacg
    11521 ggagagcgcg ctcgcatggt caaccaggcg cggctcttcg ccgcgctcgc gcgcgagggg
    11581 tcgtgggtgg acgcgcggat cgaccacggc gacccgagcc gccagccggt gccgaagccc
    11641 gacgtccgcc ggatgctcga gccgatcggc ccggtggccg tgttcggtgc gagcaacttt
    11701 ccctttgcca tctcggtcgg cgggggcgac accgtctcgg cgctcgcggc cggctgcccg
    11761 gtggtcgcca aggcgcaccc cggccacccc ggcacctccg agctgctcgg ccgcgccatc
    11821 gtctcggcgg cggagaagag cggtgttccc gccggggtct tcggcatggt ccagggaagg
    11881 ggacacgagg tgggcctcgc gctggtgcgg cacccgctca cgcgcgtggt ggcgttcacc
    11941 ggctcgcagg cgggcgggcg cgcgcttttc gacgccgcgg cggcgcggcc cgatccgatc
    12001 cccgtgtgcg ccgagatggg cagcacgaac ccggtgttcg tcctgcctgg cgcaatggcc
    12061 gagcggggcg cggagatcgc cgaaggctac gtgcagtcgg tgaccctcgg caccggccag
    12121 ttctgcacca accccggcgt gctgctggcg gtggaagggc ccgcgctcgt cgcgttcctc
    12181 gaccgcgtgg cggcggccgc tgcggcggtc gcgccctcca cgatgctcaa cgagggcatc
    12241 tgccgcgcct tccacggcgg gctcgacaga gtgcgccaga cgcatggcgt gcgcgtcctc
    12301 cgggagtcgc acgcgagagc ggatcgggcg cgggcggaag cggggtgcgc gatcttcacg
    12361 gccagcctct ccacgctcga ggcgcggccc gagctgtggg acgaggtgtt cgggccgtcc
    12421 tccatcgtga tcgtgtgccc cggggccgat gacctggaac gcgcggccag gcgccttcac
    12481 ggccacctca ccgccagcat ccacgcgacc gcggccgaac tggagtcgaa ccggcagctc
    12541 gttgccgccc tcgagcggca cgtcggccgc atcgtcttca acggtttccc gaccggcatc
    12601 gaggtgtgcg cggcgatgca ccacggcggc ccgtggcctg caacgaccga cgtgcgctac
    12661 acgtcgatcg gcaccgcgtc gatcgagcgg ttcgtccgcc cggtgtgcta ccagggcttc
    12721 ccgcagccgg cgctcccggc cgagctgcag gatgtcaaca cgcgcggcat ctggcggctg
    12781 gtggacgggg tgcgctgtcg gtgaccggga gaccttccga cctcgcgact ggggccgcta
    12841 tactcgatca gatcccatgc ccctggcccc tgcgtcccgg ctcggcccct acgaggtcgt
    12901 cgaccttctc ggcgctggcg cgttcggcga ggtctacagc gcccgcgaca cccggctcgg
    12961 ccgtcatgtc gccgtcaaga tgctctccgc cgcccgcgcg ggcgatccgc aacagctcga
    13021 gcgtctcaag caggaggcgc tgaccgccgg ctcgctcagc caccccaacg tcgtctcggt
    13081 ttacgacgtc ggcaccgagg gcgagtcgtt cttcatcgtg tctgaactgg tcgagggccg
    13141 gacgctgcgc caccacctgg tgcaggggcc gctcccgccc gggctggcgc tcgactatgc
    13201 ggcccaggcg gcgcgcggcc tggccgcggc gcacgaaaag ggcatcgttc accgcgacct
    13261 caagcccgag aacctgatgg tcacgcccga cgggcgcgtg aagatcctgg acttcggcat
    13321 cgcgaaggtc gactccaccg gggcccggct cgacgaggcc ggcctgctgc cgacgacggt
    13381 cgaggagacg ctgccgggga cggtgctggg aacggccgcc tacatgtcgc ccgagcaggt
    13441 gcggggcgag tcggtggacg gccggtcaga cctcttcagc ctcggcgcga tcctgttcga
    13501 gatgctgacg ggccggcgcg ccttcgacgg cgcaacagct gccgagacga tcgcctcgat
    13561 cctgcgcgac acgccggtcc tgccgtcgtc cgttccgccg gccgcggccg cgatcgtgag
    13621 gcgctgcctc gagaagcagc ccggacgccg gcacgagtcg gcccggcagc tggccgaggc
    13681 gctcgacgcc gtcctcggcc gatcgtcgcc cggcgtcgcg gctgcccgcc tggtgccggt
    13741 ggcggtgccc gaggaaccgc cccgcgttgc cgtgctgccg ttcaccaacc tggggcgcga
    13801 gcccgagcag gagtacttca gcgacgggct gaccgagcag atcatccacg agctgacccg
    13861 gattcgcggc gtgcggatcg tcgcctggca gtcgtccgcc aagatgaagg ggcacgagga
    13921 gaacatccac gaggcggccc ggcagctggg tgtcgagctg ctcctggtcg ggagcgtccg
    13981 gcaggcgcgc gatcgtgtcc gcatcgcggc gcggctggtg gaggtcacga cgggctacta
    14041 ccggtggtcc gagacctacg accggcagat cctcgacctg ttcgcggtgc aggaagagat
    14101 tgcccgggcc atcgcgggca ccttcgagcg caccctgggc gggagcgccg tccggcgcga
    14161 gcggcgcgcg ccggccagcc tcgaggccta cgacctctac ctgcgcgggc gcttcgtcgg
    14221 cagccagcgc accaaggccg ggctcatcgg cgcggtcgag tgcttcgagc aggcgatcgc
    14281 gctcgacgag gaattcgcgc tcgcgcacgc ggggctggct gacgcgctct gcctgcttag
    14341 cgactacggc ctcacggcgc ccgaggacgc gatgccgcgg gcgagggcgg cggcactgcg
    14401 cgcgctcgat ctcgaccccg gttcgggcga ggcgcacgcc tcgttcgcgt tgatccactc
    14461 gatttacgac tgggagtggg aggaagccga ggcgttctac cgccggtcga tcgagctgaa
    14521 cccgggctac gccaccgccc accactggtt cgcgctcgac ttccttgcca acgtcggccg
    14581 gctcgacgag gcccaggagg agctggacct ggcaaggcag ctcgaccccc tctccgcgat
    14641 catccaggag gggaagccgt acctgctgat gatgcagcgg cggtacgacg aggcgctggc
    14701 cggcttcaag gccctggcgg atcttcagcc cgggttctac aaggcctaca cgacgatggg
    14761 gcgaacctac tcgctgatgg gccgctacga agaggcgatc gcgatgctgg agcggggccg
    14821 cgagctgtcc ggcggcgagg tgcccaacat cctcggcgcg ctggggcagt cgttcgggct
    14881 ggcgggccgg cccgaccggg cccgcgagct gctcgaccag ctggcggcgc tgtcgcgcga
    14941 gaagtacgtc ccggccacct gcttcgcgct gatccacgcc ggcctcggcg agcacgacgc
    15001 cgcggtggac tggctcgagc ggggcgccga gcgccgcgac ctgccgctct cgtccatcaa
    15061 gacccacccg gcctacgaca gccttcgcga gcaccctcgc ttccaggcgc tgctcgcacg
    15121 cctgcggttt cccgggtagg actgctgtca ccgcagctgc tgccgccacg cccgcgcccg
    15181 ctggaacact tctgcgaggc ggtcgccgtg cgggaggtcg tcgcgcagcg cctcgagttc
    15241 gtcgatcagg cggcgcaggg caagcgagag gcagtcggcg ttggccgcgc agacgtcgcg
    15301 ccagacgccg gcggggctcg aggcgagacg cgtggtgtcg gcgaggccct tgccggccag
    15361 ggcgagccct tcctctcccg ccagttcgcc cgccacctcc atcagcgccg aggccacgat
    15421 ctgcggcagg tggctcacgg ccgccatgag ccggtcgtgc tccgtcgctt ccagcgttct
    15481 gggcaccgcc ccgagggcgc ccacgaattg ctcgacgcgt gaaacgacgg gcggcggcgt
    15541 gtcgggaccc ggcgtgagca gccaggggcg gccgtcgaac aggctggcgg aggcgtgggc
    15601 cagtcctccc tcggcggcgc cggcaagcgg gtgcccgccg acgaactggc gctccgcgcc
    15661 ggcccaccgc gccgcggcaa ggatgtcgcg cttggtgctg ccggcgtcgg tgagcagcgc
    15721 gtcttcgcgc gcgcggcgcg ccagcgcggg caacagcgcc gcgttggcgc cgacgggcgc
    15781 cgccagcacg atcaggtcca catcgtcgag cgatgcgagc ttcgtcgagg cgcggtccac
    15841 gacgccccga gcgagcgcat cgcgcaccac ctcctcgtcg agatccatcc cggccaccac
    15901 ggctccgggc cacgcgcgcc gcaccgccag cgccaccgag cccccgatca gcccgagccc
    15961 ggcgatgccg acgcggctaa atccggcttc gtcgccgctc atcgctccga gccctccgcc
    16021 aggcctgagc cttgagcctt tagctctgag ccctccgtcg gccctgagcc ttgagccttg
    16081 agccctgagc ccgtcttcac gcacacccgg cgtccgattg ccgggccgat gatcctcagc
    16141 tcgcccatga ggtcgtcgaa ctgcgccggg tagagcgact gcgcgccgtc gctgagcgcc
    16201 tggtcgggat tgcagtggac ctcgacgagc aggccgtcgg cgccggcggc gacggcggcg
    16261 cgtgccatcg gcaccacctt gtcgcgccgc cccgtgccgt ggcttggatc gaccacgacg
    16321 gggaggtggc tgaccttctt gactaccggg atggccgaga ggtcgagcgt attccgggtg
    16381 gcgttctcga aggtccggat gccgcgctcg cagaggatca cgtcgcggtt gccgccggcg
    16441 aggacgtact cggccgagag cagccactcc tcgatggtgg ccgagatgcc gcgcttgagc
    16501 aggaccggct tgcgcaccag gcccagctcg cgcagcagcg tgaagttctg catgttgcgg
    16561 gcgcccacct ggaggatgtc ggcgtagcgc gagaccacct cgatctgggc gacgtccatc
    16621 acctcggaga ccagcggcag gccatgccgg tcggcggcgc ggcgcagcat ctgcaggcct
    16681 tcctcgccca gcccctggaa ggcgtagggc gagctgcgcg gtttgaaggc gccgccccgg
    16741 agcagcttcg ccccggcgcg cttgacggcc gcggcggtcg cctcgacctg ctcctcggtt
    16801 tcggccgcgc acggcccggc cgccacgatc acctcgtcgc cgccgatgcg cacgtcgccc
    16861 acggtcacca ccgtgtcgtc gggcgtgaac gcccggctgg cgagcttgta cggctgcgag
    16921 acgcggagca cctcgtgcac gccgtcgagg atctcgacgc tggccgggtc gatcgctccc
    16981 gtgttgccga cgaccccgat cacggtgcgc gagacgcccg tggaccggtg cacggccagg
    17041 cgcatgcgca cgagcgcgtc gatgacccgg tcgatctgtt cctcgctggc tccctcttgc
    17101 atcaccacta ccacgatgct cctccttccc gctccgccga ttcctcgaac gccgcctcga
    17161 ggcgccgcgc ctcgtcgatg atccgctcga acaacctcgc aatcgcttcc tcgccgagcg
    17221 gcccgcccag cctcgcggtg acggcccgcg agtggcggat caccgcctgt tcccggtcgc
    17281 cctggaagat cggcagcgcg tcgcgccgct tcagccgccc gatttcgatc gcgcacgcgg
    17341 cgcgcgcgtt cagcagcgac acgagctggt cgtcgatcgc gtcgatccgg gcccgcaagt
    17401 cctcgatggt caccctgccc tcgcaccaaa agaaaaagcc ccttcaaccg gttggctgaa
    17461 gaggcttggt tggtggttct cgaaaacggt tttcgacccg cgccccttca gtgggtggta
    17521 aaccaccccc aataggtaaa cgcgtagccg aaggcctgga ggcagaccgc gtccgggcgc
    17581 gccgcgggcc cggcgggcgc gagtgccacg cgggcgacgg cggagcgagc gggacgagtc
    17641 cttgtgctca cgggacagcc gaacggtaac cgagacacgg gtggaaggtc aagcccggcc
    17701 gcctaccgcc ccttcttcac cgccatcctg acgatggtct tcaggcgctc gggggcgacg
    17761 cggcgcgggt cgttgaggta gatctcgtgg gcgggcccca ccacctgcag cccgcgcgcg
    17821 gcggtctcgt ctgccaggcg ctggtaggcc gtccccacct ggtcgtacgg gccgacgtgc
    17881 agcagttgga gcgccttccc ctcggcccag gtgatgcgct tcaccgcgtc ggtggccggc
    17941 cccttcttcg cggcaatcgc cttccgcacc tcgcgcagag tgggggcctt cacgcatggc
    18001 gggacgcgca ccatcacgcg ccagtgccac tcgcgccgcc ccttctcgca cgggttgtcc
    18061 aggtacacgc actcgagggc cgggacggtg aagtcgacgc cgcccgcctt cttcatcgtg
    18121 aacctggtcg tgtaggcgag cgtgaagagc tggtgcatcg cctcctggaa cgccgcgtcg
    18181 cccggctcgc cgacgccatc cacggccagg aacgtgccgc ccgggacgcg gacctcggcg
    18241 ggcgcgcgcc gggccccgta cacctcgggg aggctcttct tgtagtcgat cttcgccatg
    18301 acggggggcc gtgccgaacc ggtgaggggt ttcaccattt ggggccgtac cgaaccggtg
    18361 agattcttca ctcttttggt acggcccctc gtggtgaggt ttttcacact ttcggtacgg
    18421 cccccggtgg cgggcctgcg gggatggacc agcttcagct tgggcatggg cggattctaa
    18481 accggccgcg aaccgtgaca ctctcttttt ctctcccttt cgcgtctgcg cgataatcgc
    18541 caaaacccat atgcgtcacc gcaccaccac cgtcctcgcg cttttcgtaa ctttcctggt
    18601 cgccccgctc gccggccagc cggcccggct cgggtactac cgcttcccgg ccatctggcg
    18661 cgacacgctc gtcttcaccg ccgagggcga cctctggcgg gtgccgctcg ccggcggcgt
    18721 cgcgcagcgc ctcactaccc acccggccgc cgagacccgg ccggccatct cgcccgacgg
    18781 ccggacggtc gcctactcgg cctcctacga ggggccgacc gaggtctaca cgctgccgct
    18841 cgagggcggc gtccccgtgc ggcagaccta cgacggcggc aacgcgcagg tggtgggctg
    18901 gacgcccgcg ggcgagatcc tctacgcgac cacccgcttc tcgggccttc cgaacacgca
    18961 gctggcgaag atcgatcccg ccacgcgcac gcgcacgctc gtgccgctgg cgcaggcgag
    19021 cgacggcgcg tacgacgcca aggcgacgac ccttttcttc acgcgcctcg ccttccaggg
    19081 gagccacacg cggcgctaca gggggggcac ggcgcagaac ctgtggaagt tcaccgacgg
    19141 cgccgaggcg gtgccgctga ccggcgacta cgacggcacc agcaagacgc cgatgccctg
    19201 gcaggggcgc atctacttcg ccagcgatcg cgacggcgcg atgaacatct ggtcgatggc
    19261 cgaggcgggc ggcgacctgc ggcagcacac gcagcaccgc gacttcgagg tgcgctcccc
    19321 gtcgctctcc gaggggcgga ttgcctacca gctcggcgcc gacatccacg tgctcgacct
    19381 ggccagcggc aacgaccgcg cggtgccaat cacgctcgtc tccgacttcg accagatgcg
    19441 cgagaagtgg gtgaccagcc cgatcgactg ggtcacctcc gcacacctct cgcccgacgg
    19501 cgaccgggtc gcgctcacgg cccgcggcca ggtgttcgtc gcccccgccc tccagggacg
    19561 cctggtcgag gccacccgca acccgcgggt ccgctaccgc aacgcgcgct tcttcccgga
    19621 cggcaagacc gtgctggcgc tttccgacga gagcggcgag gtggagttct ggcgcgtccc
    19681 cgccaatggt gtcggctcgc cggcgcagct gacctccgac ggaaaggtgc tgcgctggga
    19741 cggcctgccg tcgcccgacg gccgcctcat cgcccaccac gacaaggacg gcctcctctg
    19801 gatctacgac atcgcgaaga agacgcagac gaaggtggcc gaggcgctcg acggccggtt
    19861 cgacgagatt cagtggtccc ccgacagccg ctggctcgcg tacgtcgtgc cggggccgaa
    19921 ccagctggcg cggatctggg tgttggaggc ggcaaccggg cgcgtcacgc cggtgaccac
    19981 cgaccgctac gacagcggca gcccggcctg gagcccggac ggcaagtggc tctacttcct
    20041 ctccgaccgc cacttcgaat cgagcgtcag cagcccgtgg ggatcgcgcc agcccgaacc
    20101 gtacttcgac aagcagacga aggtctacgc gctggcgctg aagaagggcg agcgctcgcc
    20161 cttccagccc gacgatgagc tgcacccggc gaagaaggag gaagcgaagg agccgaagaa
    20221 ggagcaggcc ggcgaggaga agcctgcttc cgcgaaggac gcgaagaagg acgtgccgaa
    20281 gggcgggaag aaggacgccg ccccggccgg caagccggac gaggccgcca aaccggccgc
    20341 ggtgccgagg gtggagatcg acctcgaagg catcgaggcg cggatcgtcg agatcccggt
    20401 tccgcccggc aactacagcg acctctcggt ggacggcaag cgcctctact acgtctcgag
    20461 agacgccggc cgcgacgcca agcggatgct gaagaccttc ccgatcgaca acaagaagcc
    20521 cgaggccgag accttcgccg aggacatcgt ctcgtacgag ctgtcgcagg acggcaagaa
    20581 gctgctgctc aggaaggcga aggacttcta cgtcctcgac gcggcggcca aggcgccggc
    20641 ggcacccgag ctggccaagc gccaggtgcc gttgaaggac tggaccttcc gcctcgaccc
    20701 gcgggaggag tgggcgcaga tgtaccagga ggcctggcgg ctcgagcgcg attacttcta
    20761 cgaccgcggg atgcacggcc tagactggcc ggcaatcaag gcgcggttcc agccgctggc
    20821 cgaacgggtg accgaccgcg aggagctgag cgacgtgctg gcgcagatgg tgagcgagct
    20881 gtccgccctg cacatcttcg tctacggcgg cgaccgccgc gaggggcagg accgggtcac
    20941 ctcggcgtcg ctcggcgcgc gcctggcgcg agacgaggcg gcgggcggat accgggtgga
    21001 gcacatctac aggtccgacg ccgaccgccc cgaccgcctt gccccgctca cccggcccgg
    21061 ggtcggcgtt ggcgagggcg atgtcatcga ggcggtcaac ggcgttccca cgctctcggt
    21121 gcccgacatc gcgagcctgc tgcggaacca ggccgacaag caggtgctgc tgcgggtgaa
    21181 gccgaagggg ggcggcgcgg cccgcgacgc gattgccgtg ccgatcagcc agtcgcgcga
    21241 gaacgagctg cgctacgacg agtgggagta cacgcggcgg cttgccgtcg agaaggcgag
    21301 cggcgggaag atcggctacg tccacctgcg cgcgatgggg agcaacgaca tcgcgcagtg
    21361 gtaccgcgag tactacccgg tgttcgaccg cgaggggctg atcatcgacg tccgccacaa
    21421 caacggcggg aacatcgaca gctgggtgct gggacgcctg ctccgcaagg cgtggttcta
    21481 ctggcagcct cgggtcggcc gcccgacctg gaacatgcag tacgccttcc gcggccacct
    21541 cgtcgtcctg tgcgaccagt ccaccgcctc ggacggcgag gcgttcgccg agggcttccg
    21601 gcgcctcggc ctcggcaagc tgatcggcac gcgcacctgg ggcggtgaga tctggctctc
    21661 gtcgagcaac gtgctggtgg accgcggcat cgcgacggcc gcggagatcg gcgtctacgg
    21721 ccccgagggg cagtggctca tcgaaggcca cggcgtcgat cccgacatcg tcgtcgacaa
    21781 cctgccgcac gcaacgttca agggagagga cgcccagctc gacgcggccg tgcagcacct
    21841 caccgagctg atcaagacga agccggtgca ggtgcccccg cctccgccct acccgatcaa
    21901 gaggggagga gagaatagag gaaaaatgta accttcactt cttcggcagg ctgcgcgtgt
    21961 cggcctcggt cagccccagg gcccacctga tcccctcgag gtacatcgtg gcgaccccct
    22021 tgtcgtccca cgcctcgtag gtgtggccaa gggtcgagta gaacacgcgc cccttgccgt
    22081 agctcttgat ccaggtgacc gcccagtcct tgtcggcccg cttgatgccc ttcttcgtca
    22141 ggtcgagctt gctctcgtcc atccgcatca gcacgcgaac cttgtcgcgc gaccagtcct
    22201 tcgcctggta gatctcgtcg ttcaccacga tcgagcggct cgtgaagtgc cgggtcatcg
    22261 ggctgccggg gtcctcgatc aggatcgggg cgtcgaaggt gttccacggg tgcccgtcga
    22321 agtacccgcc caccatctcg ccgtactccg gccacttgta gaaggtgtcg agcgcgctgt
    22381 gcacgccgac gaaccccttc ccgtcgtctt tcacgaacga gatcaggtcg gccttctgcg
    22441 actcgtccat ctcgagctcg cccgtggtgt agaaaacgac ggcatcgaac cagtcgaggt
    22501 tcttggcgtt gtcgttcccg ggcttcttct tggtgatcag ctcgcagtcg gtccggatgt
    22561 aggtgtcgaa gaggcccgtc tcgtggcccc agcgccagat cgtcgccagg ccgtaggtga
    22621 cggcgtcgtg ctgccagccc tggctttccc cgatgcagag gaccttcttc ttctcgggct
    22681 tcttcccggg cgcctgggcg gcgagggcgg cggcgagcga gacggcggcg cacgcgatgg
    22741 cgagggtgtg caggggtttc acaggttcca cctccaatcc cgatgccagt tgcgattcca
    22801 gtccgaacgc ggagctccaa tcctaatcgg aatcccaatc tccgcctacg cgcatgaccc
    22861 caccgcgaac acttcgtccc cggacgcgat gctgaagatg cgaccgccgc cgccggcggg
    22921 cgccaggcgc acctccacgg ccgcggtgct gccggggatc ccgcctccat acttgatccg
    22981 gacgctgcca ggccgcggcg ggcacccgag acgcgagagc gccgtctgca ggaggtcgag
    23041 gtacaccgtg ttgttgacgt ggtcgttcgc gtccacgtcg gaataccgga gcgacacggg
    23101 aagcgacgcg gccgccttcg aatcgggtgc gtcgaatgcg agcgcctgga gctccgggca
    23161 gaagacatca ccagaccgcg acgggaaccg gtcggcgatc tcgggcggca ctttgacgac
    23221 ggccttcgcg cgcacgctga tgtagagcca gagcgacgat gcgcgcatga ccgtctggcc
    23281 gtcggcggca tcgacccgga agtcgcggtt ccccaccgac cctcgaatgc cggtggacca
    23341 ggtttcgacg cgcagcgcct cgccgtagcg ggggtagcgg tcgatcgcca cggccatgcg
    23401 tttcagcacc cacgactcgc cgcgagtgag catggcgtcg gtgccggtgt cgtagaggtt
    23461 ggcgtgggcg atggccgcct cctgcagcag cttgaatgcc ccctggaggg tgagcaggtt
    23521 gtcgcggtcc acgtcgccga acgccacgga agaactgagg aggaagctat cggacatcct
    23581 ggtgaagata gtccgaatcc cggtgccgat tccaatcccg acagggatcg cgatcacgtt
    23641 gagcagggcc gtgagcgggt gacgcggggc ggagggcccc cgctcgcctg gagcgcgaca
    23701 cgcgtgacaa gcgcttttcg cgcgaagccg agcggggggg agccccgcgg caggacccgc
    23761 gaacccgcat ggaagtagga agattaagcg gtcaccgacc agcccggagc ggcgagacgt
    23821 aagtgagcac ggatgcaagc gttgatcagg gcgacgagtg gcgaccgtgg gcgaagaggt
    23881 tgacgtgtac aatgcggccg caaccgggga ggtgacttgt gaggaagctg ctgctggtga
    23941 ccgtcgcgct cgtcctcgtg agctggcctt cgcttgcgtt ggcacaggcg acaccggtgc
    24001 cggccccggc ggcggcggcc gcgcaggccc cggccgggtg gacactcgtc ttcgcggacg
    24061 agttcgacaa gcccggcgcc cccgaccccg cgaagtggga ttacgagctg ggctacatcc
    24121 gcaacaagga agcgcagttc tactcgtcgc ggcccgagaa cgtccgggtg gagggcggca
    24181 gcctggtgat cgaggggcgc aaggagccgt acgaggggtt cgcctacacc tcggcgagca
    24241 tcaacacgcg cggccgcttc gagttcctct acgggagggt ggaggtgcgg gcgaagctgc
    24301 cggcgggccg cggggcctgg cccgccatct ggacgctggg gaccaaccgg ggcaaggtcc
    24361 gctggccggc gtgcggcgag atcgacatca tggagaacgt cggcttcgcg ccgctgcgca
    24421 tccacgcctc ggtccacacc gccgcctaca accacgtggc cggcacgcag aagaccgcca
    24481 tcgtcgagat cgacgacccc tcgaaagact ttcacgtcta cgcgatggag tggcgcgccg
    24541 gccggatcga cgtcttcgtc gacggcagga agtacttcac cttcgagaac gagggcaccg
    24601 gcaacgacgc ctggccgttc gacaagcccc agtacctgct gatcaacctg gccatcggcg
    24661 gcatgtgggg cggccaggag ggaatcgacg attcgagctt cccccagcgg tacctggtgg
    24721 actacgtgcg gatctaccga tagactgcgc cgctatgaac cgacggcaag cgctcgcccg
    24781 actcgcggcg atcccggcgg cggcccgcct ggcaggagcc gccgcacacg cggcgcccac
    24841 caaccgcaac gtcctcttca tcctgaccga cgaccatcgc tacgacgcga tgggcttcat
    24901 gaagccgcag ccgttcctgg agacgccgaa cctcgatcgg ctcgcccggg aaggggcgca
    24961 cttccagaac gccttcgtga cgaccgcgct ctgctcgccg agccgggcca gcatcctcac
    25021 ggggatgtac gcccaccgcc acaagatcgt cgacaacaac acggcaatcc cgcccggcac
    25081 cgagttcttc ccccaacgcc ttcagcaggc gggctacaag accgccttca tcggcaagtg
    25141 gcacatgggc aacagcggcg accagccgca gccgggcttc gaccactggg tcagcttcca
    25201 gggccagggc tcgtaccggc ccgtgccttc ggggctcaac gtggacggga agaaggtgcc
    25261 ccagaagggg tacatcaccg acgagctgac cgactacgcg gtcgagtggc tgcggtcgct
    25321 gccggcgacg cagccctggt tcatgtacgt ctcgcacaag gcggtgcact cggagttcgt
    25381 ccccgcgccc cggcacgccg ggcgctacag gaacgcgacc ttcgtgccgc cgaagacgat
    25441 ggccgcgacg ggcgacatgg ccaggggacg gccgatgtgg gtgcagaacc agaggaactc
    25501 ctggcacggg gtggagttcc cgtaccattc cgacctcgac atcggcgagt actacaagca
    25561 gtacgccgag acgctgctcg ccgtggacga cagcgtcggg cgcctgctcg acgagctgga
    25621 gaagcggggc cagctcgatt cgacgctcgt cgtcttcatg ggcgacaacg ggttcgcctt
    25681 cggcgagcac ggcctgatcg acaagcgcac ggcctacgag gagtcgatgc gggtgccgct
    25741 gctggcgcgc tgtcccgagc tgttcggcgg gggccgcgtg gtgacgccga tggtggccgg
    25801 actcgacatc atgccgaccg tgctcgacgc gtgcggcgcg gccatcccgg gcggcatcga
    25861 cgggcgcagc tggctgccgc tggtgagggg gaacgcggag gggtggcgca ccgagctgct
    25921 ctacgagtac tactgggagc ggaacttccc gcagacgccg acggtgcacg cgctgcgcac
    25981 cgaccgctac aagttcacgc gctgccacgg cctctgggac atcgacgagc tgtacgacct
    26041 ggccgacgac ccgctcgaat cgaacaacct gatcttcagc cccgcgcacc agccgaccgt
    26101 ccgcacgatg cgcgcgcggc tgttccagct gctcgaggag acgggcggga tgagcatccc
    26161 gctgtacccg gacatgggat tccaggggaa cctgcgacac cccgaccgct cgcccgcggc
    26221 ggagttcccg ccggagctgg cgccgcggag aaagcggtag gcgacgggag gctcagggct
    26281 cacggctcac ggctcaagga ctagggccgt gggcgacaac tgacgccgtc gcatttggag
    26341 ccgcgggccg ttcgcggcga aaaccgtgtg gagcaggccg atcaggtgcg gacaaaacgc
    26401 accgcaggcc tgcggacagg cctggggatg ggggccccca ggcgcgttcg gacggacggt
    26461 aagccgaggg gcgttttgcc ggcctgcgga
//
LOCUS       DAOPQX010000009        26281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2379_length_26281_cov_4.696980, whole genome shotgun sequence.
ACCESSION   DAOPQX010000009 DAOPQX010000000
VERSION     DAOPQX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 26281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26281
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2379_length_26281_cov_4.696980"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2054
                     /locus_tag="PKK95_01075"
     CDS             <1..2054
                     /locus_tag="PKK95_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC16824.1"
                     /translation="GRSMEQGGDRDAWPETADLLGDAMTVAPPSSREQTPHRGEAGVP
                     ASHLETERGALLGTLAYMSPEQARGERATAASDLYSLGLLLQELFTGKPPLALDADSA
                     TLLERARRGDVPSPKGIDKDLARLIQRLKSPAPSERPTAVDTLERLRWIAAKPARRLR
                     RLAIAAALLVAALAGTKYFVDLERERSAAVAARNQAEGRRAQAESLVGFMIGDLRKKL
                     ETVGRLEILDEVGAKAIDYFAAVPESELTDDELAGRSAALYQIGDVRIAQGRLAEAGE
                     PLTQSLALAKALADRHPGDGARLFNLAQSHFWVGFVEWRRRRLDEALVHFREYLRIAE
                     RLVGLDPKSMEWRLELSSANSNIGSVLEEQGRPDAALERFEACLAIETALLVERPDDA
                     TLRGAAAASHNTIGAVLRTLGRLDESLAHHRQELAFHEELVHREPGTVQWRQFLSVSH
                     NRVAMLLEATGSLEAAAAHAAEALAIAGRLSAEDPSNLDWLRELGRSHHRAGAIAGAR
                     GKAAEALTHLRSGADTMQRLVRLDPLNPSRRRDLADARAALALQFLSAGQAERARREA
                     RLVEEIAGGLLRQAPDDLQAVRLRGLSLVLQGRAWRAEGSQARAEEALGRAVETLEPV
                     ARSSRDYSVLEPFALALAGLGRLGESRGVVRQLAAMGYRNPLLAAAVNGSGAAMVPPA
                     R"
     gene            2080..2394
                     /locus_tag="PKK95_01080"
     CDS             2080..2394
                     /locus_tag="PKK95_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16825.1"
                     /translation="MPPIEVTVSWPAGAAGPTVDKDPIDVPRGAGATVIRWVCGDNVS
                     RLQISGLDGTVFSPAASQGMVPSFSTTDANREAGTYSYTLAATQTSGDSTAGDPRIRN
                     GG"
     gene            2496..4235
                     /locus_tag="PKK95_01085"
     CDS             2496..4235
                     /locus_tag="PKK95_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC16826.1"
                     /translation="MAGDRAPLLDEIFEFRPDAGLIRLHDQRVVILSAAAMGLLRKEL
                     IETLGEPTARRLLLRFGFADGYHDAINLRDQLGGRSALDRVRAGARLHALEGIVLGEM
                     GRVEYDAGQGRFRCPLVWRGSYEAEQHREHFGTSTEPVCWSLVGYISGYTSASLGHEV
                     YFRERKCLGQGHSHCTLLGQDASSWGDELESLRFDFQGADLGAEVERLRVAAGRQRSE
                     LARREEALARREREMDLLQQRVALHVASQRFVVRSAALREVVEMASRVAPLDTTVLVA
                     GESGTGKEFIVRLIHEHSARAGGPLVSVNCAALTETLLESELFGHVRGAFTGAVRDKP
                     GLFEVANHGTLFLDEVGEIAPALQAKLLRAIQEGEIRRVGGERTLKARPRIIAATNRD
                     LRTAVGEGRFREDLYFRLGGFVIVLPPLRDRREDIPALVHEFVRHAAERLQKAVAGIS
                     PAAMTALLNYGWPGNVRELEHAIERAVILARGRTLGLEDLPPEIVEARDAPSRPGADL
                     RLASHTRAMVVEALRRTGGSRKEAASALGVSTVTLWRMMRRYGLLDGPEVKTAGKREG
                     EAGRGRTGARISR"
     gene            4425..4802
                     /locus_tag="PKK95_01090"
     CDS             4425..4802
                     /locus_tag="PKK95_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16827.1"
                     /translation="MRLDIQGSALSRQADIRRYIERRALLEFGRFGDRVDTIRVRVIE
                     RPNEPRGRFFCGLAVTISPEEEGLQPRHVLARRQDEDVRAAVDSVIERAGQLTAGELL
                     RADAARAAREQWRPVLASVGGES"
     gene            4799..5062
                     /locus_tag="PKK95_01095"
     CDS             4799..5062
                     /locus_tag="PKK95_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16828.1"
                     /translation="MNPYQFLAQQPGVGDVRDLVQQLGAWHDSMVLHQRTVRQVGAEE
                     ACDESCAHAVGRDLWIQAKMRLGPVAETLTFLRRSVERTTRES"
     gene            complement(5111..6904)
                     /gene="treZ"
                     /locus_tag="PKK95_01100"
     CDS             complement(5111..6904)
                     /gene="treZ"
                     /locus_tag="PKK95_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOC16829.1"
                     /translation="MGAPFARRLLIHLEGAGLPIALEPAPHGLFEGFVPGLGAGARYQ
                     LQADDMVPMPDPGSRFQPGGVHGPSEVIDPRFEWTDAAWRGPDPRALRIYELHVGTFS
                     RAGTYDEVASRLPYLADLGVNVIELMPIADFPGNRNWGYDPGALFAPARCYGRPERLR
                     RLVNEAHALGIAVILDVVYNHLGPDGAYLAAFMPSFFSEEREGPWGRAVNLDGPGSEL
                     VRWFILENARYWIRDFHLDGLRLDATHTLEDRSPRHLLEELGAAVREEARAAGRRAIV
                     IAEDDRNLDRLVRGEAERGYGLDAVWADDFHHQVRSATAGDKDGYYADYTGTARDLAA
                     TARRGWFYTGQYSRHHKRARGTNPSGVPLERFVYCIQNHDQVGNRAVGGRLNHQVDLP
                     VYLAASLLLLALPETPLLFMGQEWAAGTPFLYFTDHGPELGRLVTEGRRREFSGFAAF
                     ADPAARDAVPDPQDEATFEASRLRWEETQVEPHAGVLRFYRAMLGLRGHHPLLQASVP
                     SRVEAMGEASVGVWRGEAGEGDLKLAALVCLAGVERVELPVTGSGAELLLSTEDPSFM
                     PDARPGNLDARPGRIIVEFARPGGAILLLGP"
     gene            complement(7056..7466)
                     /locus_tag="PKK95_01105"
     CDS             complement(7056..7466)
                     /locus_tag="PKK95_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOC16830.1"
                     /translation="MSEQTPPSPAGVRLDVWLDVACLFRTRSEAQQACSGGKVEVNGQ
                     AGKPHRPVHVGDRLRITRAHGRRQVVVVRELAEKHVPKAAARALYEDQTPPPTPEELE
                     QIELARAFRRSFGPRPSRAPDKRARRELRRLKGQ"
     gene            7753..8043
                     /locus_tag="PKK95_01110"
     CDS             7753..8043
                     /locus_tag="PKK95_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16831.1"
                     /translation="MAPTLLPHVTSRAEYSLKGQEQVTGMGSSALRIRETLERIQGEF
                     RELPGLRLTLAQAQRLWRLDKNVCDALLAALVDARFLARTADGAFIRGTAVN"
     gene            complement(8227..8871)
                     /gene="rdgB"
                     /locus_tag="PKK95_01115"
     CDS             complement(8227..8871)
                     /gene="rdgB"
                     /locus_tag="PKK95_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOC16832.1"
                     /translation="MIAELVIATGNAGKVREIRRVLDGLGLTLLSLADLDPVPEPEET
                     GQTFAENARAKALAYASALGRMVVAEDSGLEIDDLDGVPGVYSARFGLPGAPSYPQKF
                     ELLYRMLRERSGRVDSDARFVCALALAAPAGPEGAAASLPRIVFEATGTVEGRIIEPP
                     RGAGGFGYDPIFYYPPFGQTLAEVPAGRKEEVSHRGKAFRKLRKYLEPSLRREP"
     gene            complement(8868..9602)
                     /gene="rph"
                     /locus_tag="PKK95_01120"
     CDS             complement(8868..9602)
                     /gene="rph"
                     /locus_tag="PKK95_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOC16833.1"
                     /translation="MTRSDQRTPSTLRPTRLTPHFVKHAEGSVLIEAGDTRVVCTASV
                     EDRVPPFRRGSGKGWVTAEYGMLPRSTTTRSVREASAGKVGGRTQEIQRLIGRSLRAV
                     TRMEELGERTIWVDCDVIQADGGTRTAAITGGFVALALALQKLHAQGQLAAVPLQDLV
                     AATSVGIVDGVPLLDLAYEEDSRAEVDMNVVQTGDGRLIEVQGTAEAAPFGRDALMRM
                     LDLAAEGIATLNRLQLEVLGDIRFRR"
     gene            complement(9619..10209)
                     /locus_tag="PKK95_01125"
     CDS             complement(9619..10209)
                     /locus_tag="PKK95_01125"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOC16834.1"
                     /translation="MRRSLIVWIAVMAAVVAAAGLGAVVLSRWLASGVEAPPSEGGPA
                     AASADEARKIKARLFYVAEDGVHLVPVEREVPFGATTADQARRIVEAQLEPAPEGLVS
                     PIPAGTRLRALYLTEANQAFVDLSAEARSAHPGGLTNEILTVYSVVDSLTVNLPVLTG
                     VQILVDGHEVDTLAGHVDLRRPLVRSTFWIDEGTPR"
     gene            complement(10206..11783)
                     /locus_tag="PKK95_01130"
     CDS             complement(10206..11783)
                     /locus_tag="PKK95_01130"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC16835.1"
                     /translation="MFLPRSRLLIVAVLSASLAGLSPHPGLAGQAAGARPAPAFTVIS
                     PQARTPLPASQAGDQLMVALDDLAPLFGLTVREDGIGGGLTVTAANGKRVLLTPGQPL
                     ASADGRLVSLPAAPVRDGRRWLVPIEFLSRAIAPVAGTPIEVRRGSRLVLVGDVRVPR
                     VEVRQQEAGGVIRLTLTVAPRAGHQVTHEGNRLLLRFDATALDPALPAVTPGPLLQGI
                     RLGDTPATLAVDLGPRFGSYRASAATRDANSEVIVELVPSFTDQAIPVPPPSPPAAEP
                     LVNLAPPRPSVRTVVIDAGHGGDEQGASGPSGALEKEVALAVARRLRAVLEGRLGLRV
                     LMTRDADKAVSLDERAALANTNKADLFISLHANASLRPATRGAQVYFLSAGQGGEASR
                     AALAGGALPTLGGGSRDVELILWDLAQVRHLDESAAFAAILEEELRGRIAVNERPVLQ
                     APFRVLAAANMPAVVVETGYLTNTEDERLLKSAGHQDLIVQALLQSVIRFREVRERGV
                     AAMPTGVSAPNPSRPPR"
     gene            11906..12619
                     /locus_tag="PKK95_01135"
     CDS             11906..12619
                     /locus_tag="PKK95_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16836.1"
                     /translation="MSLRLMRPALVFLAMFVNLPGVHAQGWLVQGYPAGVQVGAGAVA
                     PVGEAAGSSGIGWNVGGGGELEFRPGLAIRAGYFYGRFAAEDATVVAESGSGALRGKT
                     QMHAGTVDLVWKRPLTGREATVYLFAGPVIAYRRVTVTSPSGVALAESPVSFCEPHWL
                     QCSPTSLPFHRLLGIRRSTDVGASAGAGVSFDVGLRARLFAEARFVFLDGPSFRDASG
                     SKRSADAFYVPVFLGVRFQ"
     gene            12731..14902
                     /locus_tag="PKK95_01140"
     CDS             12731..14902
                     /locus_tag="PKK95_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC16837.1"
                     /translation="MFARRSCPWWGIALLVFVALGSQALPEASQDDPVVAKIIQLGKT
                     DNQVMVWNDYASNRFGGRETGTNAYRDATEWAVWQFRQWGFEAELDEAGELPVGFNRG
                     PWFGRMRTPVEKALRFGTPSFTAGTKGIQRGGVAILKADPFSVPGRAQGGVPLPKETV
                     EKKRAAVEAALAEIAANKASYNGKWVLIPGASTGFARDGRRGTKLPDGSFEYLDAEMI
                     PPVTRALVEAGALGTIQSSKPPAPAQDGFLPPLSILDGFVPSWDALPVLPDIKLLDTQ
                     YDEIKALVEKGQPVELEFDIRNHFKMGPLKYHNVVATLRGTTYPDEYIVIGGHLDAFS
                     VATGGVDDGSGFSPNMEAMRLLAKAGARPKRTIVCVLFAAEESGLLGSQAWLRNRPGI
                     ESKIVMMINRDGSPSAITGASVPPAWYDDFARITAPLETANPRWPFKLVTNEYPAPRP
                     ASPGGSDHSSFQMRGVPTLGVQTSSVLPAVNGEKERNFVYPYVWHTTNDLYDQLVPYT
                     EHQQHSALATAVLAYGVANLDKPLPREGVFLPDGLYADITIGGGDAPKRVIASLDFAN
                     APLHAAAFVRLFEGNKPGSAAQAARPPAAALAMMGQRRPPEIAPVGRILDVRGRVTGV
                     ITSELGRSMAVRNLPKRANRVLKQDAPGVLAMAGPSTFYLTSGRKGGGGVAIGQVIAG
                     VKTLPTVGKDDPIRSVRILRVGQAARDFNTSDEGFKELMGK"
     gene            complement(14974..17328)
                     /locus_tag="PKK95_01145"
     CDS             complement(14974..17328)
                     /locus_tag="PKK95_01145"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOC16838.1"
                     /translation="MPTLLLALLLVLLSWQPAAAATEHPPDTRTLSPYFFVHGDDPST
                     DRLPLKATSAQVDIAGVIARVKVTQVYRNEGKHALEAIYVFPGSTRAAVHGMKMTIGD
                     RVITARIEERQEARRQYQQARESGQSASLLEQQRPNVFQMNVANILPGDEIRVELVYS
                     ELLVPERGVYEFVYPTVVGPRYSTSDAATAPEPERWVQNPYLRSGEADRTRFDFSASI
                     SGGMALKRVTSPSHALRIDYPSATRARLTLGDEAAAANKDLVIRYQLADDRIETGLML
                     YESGGERFFLCLVEPPRRFSPALVPPREYVFIVDVSGSMHGFPLETTKALLRNLFGRL
                     QPDDSFNILFFSGGSYVLSPASLPATEVNKARALQEIERQTGGGGTELLPALRRAFDL
                     PRARRDVARTIVIATDGYVNVEREAFALVRDRLDDASVFAFGIGGSVNRHLVEGLARA
                     GEGLPFVVLGPGEAPRAASRFLEYIESPLLTGIDVRTPGFDAYDLEPGKIPDVFAARP
                     LVLAGKYRGEPAGEIVVSGFTGEGRFERRVKVDRSRLVTGAEALRYLWARERLAVLDL
                     AGTEAGATGAGTEAGATGAEAARKAAITKLGLEYGLLTEYTSFVAIDQRRRRQDGRLE
                     TVKQPLPLPEGVSDLAVGGMPGVAKMQVAAESLSLASPRRAEDRGRGTVLSTAPDLVS
                     PPRQAQASVRVRLLENRLTGRFVRSETITVTVQRALSAAGCTPETGTLRLRLVFGGGG
                     AVERVEAVTGANAVRPAPECVRNALQGLRLAGVRGGSVVLDVGW"
     gene            complement(17399..18685)
                     /locus_tag="PKK95_01150"
     CDS             complement(17399..18685)
                     /locus_tag="PKK95_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOC16839.1"
                     /translation="MARDRDRDAEMDRLLRAAMSDGPAGSADACPAADELSAYVEQTL
                     ARPEREALEPHLASCERCQQALAMMATMPEPAAIPANAPARTPSWWLAGWRRWLVPAT
                     ALASAAIIYVALKPAAVLQPNLSPDSIVATAPAESPTPPEAPGTSQLLPGKAVPSAAQ
                     LAEAASPRAKREEGNEEEKEAVNETPRVLARTRDKAVADRPEAKLEAAPFRADEAEAG
                     QSATLAPVPQLAAAPVAAPPSQPPPSAEPEAMAEAQAHTAQPRAAGGALGVRPTRLKA
                     GEAAAPRVAAPTGSKAAADAALAAPEQVVSTARGEGSQAAIVKGAGARPLLWRLDPGA
                     RIFGSADAGRTWQLQYTGAGRLVAGSSPSPEVCWVVGGRGLVLRTTDGRTWHAVPFPE
                     RLDLVAVLADSDRRATVHASDGRRFTTDDGGATWRE"
     gene            complement(18758..19627)
                     /locus_tag="PKK95_01155"
     CDS             complement(18758..19627)
                     /locus_tag="PKK95_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC16840.1"
                     /translation="MRGSRASTAAAEIAPALVRRLYDRAGAARWGLAEADFEASLRRS
                     LAHRFREAPPSEREAEEYLGGLHLADLALAGACARGSEPAWEHFVHDFRPALLAAASA
                     AGPGAGRELADSLYADLFGTEERDGVRRSLFDYFHGRCSLNGWLRAVLAQRAVDRARA
                     SRRLEPLPEPGTPGEPSVPAQPPEVDRSRRLVAVRAALAAALQQLPARDRLRLSLYYA
                     RELKLAAVGRVLGESEATVSRKLARCRTGLRQEVERRLRERHGMNDTQVADAFELART
                     DPAFDLARALPPE"
     gene            19797..21554
                     /locus_tag="PKK95_01160"
     CDS             19797..21554
                     /locus_tag="PKK95_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOC16841.1"
                     /translation="MLNRRLLLPALLILTLAGAVGSAQGPAHGTAKRAPGRTSADVLT
                     RPELTGYRETSRYDDVMAFMEAMAAASPQIHLTTYGYTYEGRSLPLAVIGAPAATPEA
                     VRATGKTRIYIQGNIHAGEVEGKEAALWLLRSIAKGERAAWFRSVVLLINPIYNADGN
                     EKVNLRNRGRQYGPIGGMGQRYNAQNLDLNRDCTKLETPEGRSLAKLLTDYDPHVAVD
                     LHTTNGSPHGYYLTYQTSVSPNTSPGLTALARGRLLPFITAQVKAKHGWDYFYYGNLS
                     RQGERAWYNDLDLYKPRYTQTYFGLRNRVGLLVETYSYASFEDRIKSNYWFLEELVNF
                     AAANGEAIRSATAAADAESIVGRQQAVRGRLVKDPDPVPIVLADSVDERNPYVPDRPM
                     LRRVAGTERVETMPHYATVEAAETSLAPRAYLVPAVPPPAGEKADSGRPPFPGGPPAA
                     GRGPQPASPFRDNQQMLARIVDRLTAHGVRFHRLESERTVAAEQFRIEASTVQEQSWQ
                     NMKIREITGSWEATGAKLPAGSLVVPMDQPLARLVFVLLDARSDDGLMAWGLLDDVLA
                     RTPEPAVYPVLRTMTAVPE"
     gene            21598..22224
                     /locus_tag="PKK95_01165"
     CDS             21598..22224
                     /locus_tag="PKK95_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC16842.1"
                     /translation="MNVDHIHWLGHATFRLEDGGSQIYFDPWKLPAGAPRADVILITH
                     AHYDHYSEDDIARIEQPGTLFVAPPDVAAKLKGRKVTSAEPGGTYRVGPVTVEATPAY
                     NLDKDYHKKREHWVGYIVTLSSGQRIYHSGDTDAIPEMDDVEADVALMPCGGTYTMTA
                     AEMAEAANRFKPSVLIPMHWGDIVGQKSDAEAVAKAFKGRTVIKQVEK"
     gene            complement(22274..22474)
                     /locus_tag="PKK95_01170"
                     /pseudo
     CDS             complement(22274..22474)
                     /locus_tag="PKK95_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(22490..22936)
                     /locus_tag="PKK95_01175"
     CDS             complement(22490..22936)
                     /locus_tag="PKK95_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOC16843.1"
                     /translation="MPLLDDKVRKQVRDALAAIERPVKIVMFTQGEGGALECDYCSET
                     RELVEEVSQQSEKLSLEVLDFQADEARAQEYGVDKIPAVVLLADGGATHHGVRLYGIP
                     AGYEFSSLVEGLRMVGTGDVKLADATRQALTRLTKPVHLQVFVTPT"
     gene            23314..25320
                     /locus_tag="PKK95_01180"
     CDS             23314..25320
                     /locus_tag="PKK95_01180"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOC16844.1"
                     /translation="MIAVLLVPAWAGAQTVTNARIVEFVPSPDHSERLSDGRAVVQRY
                     VMEVYQAGASQPFHTVDMGKPSPQSDGKIRFDFSATVAAWPAPGGEYQARVAAVGPGG
                     EGVSDPSNRFTVSSCSYTLSRSSALFPAGGGGGEVAVTTTSGCAWTASEGLSWVTMMT
                     AGATGSGRATFSVAANSGTSARAGTITVAGQAFQVTQEGSCSYSLSPSTASFAASGGT
                     GSISITAGSGCAWTAAASASWVSVSTASGSGAGTVRYTVAANTSTSSRSATVTVAGRV
                     HTISQSAASGSGGGTPATSLPSPWTNGDVGSVGLSGSASYSSGVFTVVGAGADIWGSA
                     DSFHFVRRSLAGDADFVARVTSVQNTNSLAKAGIMLRATTASNSQHVILDVKPNGGVE
                     FMTRTSTGGSTAYVAGTTQGFPAWLRLARRGSTITASVSSDGRTWRTVGSRSLSLPST
                     VQAGLVVNSHNASVRNTSRFDQVSVAAASAEAPAASTGLPSPWQTLDIGSVGRAGSAT
                     YSSGLFTVRGAGADIWGTADAFRFVYRPQLSGGEIVARVTYVQGTHSLAKAGIILRDG
                     TGANARHVTLSVKPNGGVEFLRRASTSGSTAYLGGTTQAFPVWVRLRRSSSTVTAAVS
                     ADGTTWRTVGSTTISTTTPLMGLIVSSHDTGVLNLSKFDRVTVR"
     gene            complement(25393..>26281)
                     /locus_tag="PKK95_01185"
     CDS             complement(25393..>26281)
                     /locus_tag="PKK95_01185"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOC16845.1"
                     /translation="VRQAYSPANAAEEARGPRGGGPAAAGRPGGGEPQINLEREKLNL
                     TESLAFMVEQLGVDHAITKRILGGATPAARAAELVDKTRLTDPEIRNLLRAGGRGAID
                     VSTDPFIVLARSLEPDAQAIAKKYEEQVTTVQAAAYPKIGQAVFVVYGQSAYPDATGT
                     LRLSYGTVKGYMENGKKVTPYTTIAGLYQRAAEHGNEPPYDLAPRWAEAKAALNLKTP
                     FNLVTTNDIVGGNSGSAMVNRKGEVVGLIFDGNLQMLPGYFIYEESINRAVSVDSRAI
                     LEALEKVYKAAELVKEIRG"
BASE COUNT         3814 a         9285 c         9440 g         3742 t
ORIGIN      
        1 gcggacgctc gatggagcag ggcggcgacc gcgatgcctg gcccgagacg gcggacctcc
       61 ttggcgatgc gatgacggtc gcgccgccgt cgtcgaggga gcagacgccc catcgcggcg
      121 aggcgggagt gcccgcttcg cacctcgaga cggagagggg ggcgctcctc ggcacgctcg
      181 cctacatgag ccccgagcag gcccgcggcg aacgcgccac ggcggccagc gacctctact
      241 ctctcggcct gctgctgcag gagctgttca ccgggaagcc gccgctcgcg ctcgacgccg
      301 actccgcgac gctgctcgag cgcgcgcgcc gcggggacgt gccgtcgccg aagggcatcg
      361 acaaggacct tgcccggctg atccagcgcc tgaagtcgcc cgccccctcc gagcgcccca
      421 ccgcggtgga cacgctcgag cggctgcgct ggattgccgc aaagccggcc cggcgccttc
      481 gacgactggc gatcgcggcg gcactcctgg tggcggcgct ggccggcacg aagtacttcg
      541 tggatctcga gcgagagagg tcggcggcgg tcgcggccag gaaccaggcg gaggggcgcc
      601 gggcgcaggc ggagagcctg gtcgggttca tgatcggcga cttgcggaag aagctcgaga
      661 ccgtcgggcg gctggagatc ctcgacgagg tcggcgcgaa agcaatcgac tacttcgccg
      721 cggtccccga gtcggagctg accgatgacg aactggccgg ccgctcggcg gcgctctacc
      781 agatcggcga cgtccggatc gcgcagggac gcctggccga ggcgggagag cccctgacgc
      841 agtcgctcgc cctggcaaag gcgctcgccg accgccaccc cggcgacggc gcacggctct
      901 tcaacctggc ccagagccac ttctgggtgg ggttcgtcga atggcgccgg cgccgcctgg
      961 acgaggcgct cgtgcacttc cgcgagtacc tccggatcgc ggagcgcctg gtcggcctcg
     1021 acccgaaaag catggagtgg cgcctcgagc tgtcgtcggc gaacagcaac atcggctcgg
     1081 tcctggagga gcaaggccgc ccggatgcgg ccctcgagcg cttcgaggcc tgcctcgcga
     1141 tcgagaccgc gctgctcgtc gagcgccccg acgacgcgac gctccgaggc gcggcggccg
     1201 cgtcgcacaa caccatcggc gcggtgctgc gcaccctcgg ccggctcgac gagtcgctcg
     1261 cgcaccacag gcaggagctg gcgttccacg aagagctggt ccaccgcgag ccgggcaccg
     1321 tgcagtggcg gcagttcctg agcgtcagcc acaaccgggt ggcgatgctg ctcgaggcca
     1381 ccggctcgtt ggaggcggcg gcggcgcacg cggccgaggc gctcgcgatc gccgggcggc
     1441 tgagcgcgga ggacccgtcg aacctcgatt ggctgcgcga gctgggacgc agccaccacc
     1501 gcgcgggggc gatcgccggc gcgaggggca aggcggcgga agcgctgacg cacctgcggt
     1561 cgggggccga cacgatgcag cgcctggtgc ggctcgaccc gctcaatccc tcgcgccgcc
     1621 gcgacctcgc cgacgcgcgc gcggcgctgg ccctgcagtt cctctccgcg ggccaggcgg
     1681 aacgcgcacg gcgcgaagcc cgcctggtcg aggagatcgc gggcggcctg ctccgccagg
     1741 cccctgacga ccttcaggcc gtgcggcttc gaggactgag cctggtgctg cagggccgcg
     1801 catggcgggc ggaaggaagc caggcgcgcg cggaggaggc cctcggccgt gcagtcgaga
     1861 cgctggaacc ggtcgcgcga tcgtcgcgcg actactcggt actcgagccc ttcgcgctcg
     1921 cgctcgccgg cctcggccgg ctcggcgagt cgcgaggcgt ggtgcgtcag ttggccgcga
     1981 tgggctatcg caaccccctg ctcgctgccg cggtcaacgg gtcgggtgcg gcgatggtgc
     2041 cgcccgcccg gtgaccttca cgtgaaggag aagagacaga tgcccccaat cgaagtcacc
     2101 gtctcgtggc cggcgggcgc cgccggtccc accgtggaca aggacccgat cgacgtgccg
     2161 cggggcgcgg gcgccaccgt gatcaggtgg gtatgcggcg acaacgtgtc gaggctgcag
     2221 atcagcggcc tcgacggcac cgtgttctcg ccggcggcgt cgcaggggat ggtgcccagc
     2281 ttctcgacca ccgacgccaa ccgcgaggcg ggcacctact cgtatacgct cgcggcgacg
     2341 cagacctcgg gcgacagcac ggccggcgac ccccgcatcc ggaacggcgg gtgagaagaa
     2401 gggttacggc cggcacggag gccggcgcga ctggcggcga atctctgctc cgcagcgctt
     2461 gtatttcatg ttgaaatacg gcgggagggt tcgccatggc cggtgaccgc gcgccgcttc
     2521 tcgacgagat cttcgagttc cgccccgacg ccgggctgat ccgcctgcac gaccagcgcg
     2581 tggtcatcct gagcgcggcc gcgatgggcc tgctgcgcaa ggagctgatc gaaacgctcg
     2641 gcgagccgac cgcgcgccgc ctgctgctgc ggttcgggtt cgccgacggc taccacgacg
     2701 cgatcaacct gcgcgaccag ctcggcggac gaagcgcgct cgaccgggtg cgtgccggcg
     2761 cccggctgca cgcgctcgag gggatcgtgc tcggcgagat ggggcgggtc gagtacgacg
     2821 ccggccaggg gcggttccgc tgcccgctgg tgtggcgcgg gtcgtacgag gccgagcagc
     2881 accgggagca cttcgggacg agcaccgagc cggtgtgctg gagcctcgtg ggctacatca
     2941 gcgggtacac gagcgcctcg ctcgggcacg aggtgtactt ccgcgagcgg aagtgcctcg
     3001 ggcaggggca ctcgcactgc acgctgctcg ggcaggacgc ctcgtcgtgg ggcgacgagc
     3061 tcgagtcgct gcggttcgac ttccaggggg ccgacctggg cgccgaggtc gagcgcctgc
     3121 gggtggccgc gggccggcag cgcagcgagc tggcgcgccg cgaggaggcg ctggcccggc
     3181 gcgagcgcga gatggacctg ctgcagcagc gggtcgcact gcacgtggcc agccagcgct
     3241 tcgtcgtccg cagcgccgcg ctccgcgagg tcgtggagat ggcctcgcgc gtggcgccgc
     3301 tcgacaccac ggtgctggtg gcgggcgaga gcggcaccgg caaggagttc atcgtccgcc
     3361 tgatccacga gcacagcgcg cgcgccggcg gccccctcgt ctcggtcaac tgcgcggcgc
     3421 tcaccgagac gctgctcgag tcggagctgt tcggccacgt gcgcggcgcg ttcaccggcg
     3481 ccgtgcgcga caagcccggc ctcttcgagg tggccaacca cgggacgctg ttcctcgacg
     3541 aggtcggcga gattgccccg gcgctgcagg ccaagctttt gcgcgcgatc caggaggggg
     3601 agatccgccg ggtcggcgga gagcgcacgc tgaaagcgcg gccgcggatc atcgcggcca
     3661 ccaaccgcga cctgcgcacg gccgtcggcg aggggcgctt ccgcgaggat ctgtacttcc
     3721 ggctcggggg attcgtgatc gtcctgccgc cgctgcgcga ccgccgcgag gacatccccg
     3781 cgctggtgca cgagttcgta cggcacgcgg ccgagcggct gcagaaggcg gtggcgggga
     3841 tctcgccggc cgcgatgacg gcgctcctca actacggctg gccggggaac gtccgcgagc
     3901 tggagcacgc gatcgagcgc gccgtgatcc tcgcgcgcgg gcgaacgctc ggcctcgaag
     3961 acctgccccc ggagatcgtg gaggcccgcg acgcgccgtc ccgccccggg gccgaccttc
     4021 gcctcgccag ccacacgcgc gccatggtgg tcgaggcgct ccgccggacg ggcggcagcc
     4081 gcaaggaggc cgcctcggcg ctcggcgtca gcacggtgac cctctggcgg atgatgcggc
     4141 ggtacggcct gctcgacggg cccgaggtga agacggccgg aaagcgggag ggggaggcgg
     4201 gcaggggtcg aacgggtgcg cgcatttcaa ggtgaatgct ggtctttcat tatgaatttc
     4261 gcgccgtccc ggccgctctg cctgtcgagc cgtaaatagc atgattgcaa tgatttgacg
     4321 agacaatcgc gcatgcctga gttggccgcc aaattgcctt gcttcaaggc aggcgggcac
     4381 tcgcccgtcg cgggaccgtt acccgaagga ggtcgaagaa gggcatgcga ctggacatcc
     4441 agggaagcgc gctgtcgcgc caggctgaca tccgccgtta catcgagcgg cgggccctcc
     4501 tcgagttcgg ccgattcggc gaccgggtgg acacgatccg ggtgcgcgtc atcgagcgcc
     4561 ccaacgagcc gcgcggccgc ttcttctgcg ggctggcggt gacgatctcg ccggaagagg
     4621 aggggctgca gccgcgccac gtgctggcgc ggcgtcagga cgaggacgtc agggcggccg
     4681 tcgacagcgt gatcgagcgc gcggggcagc tgacggccgg ggaactgctg cgcgccgacg
     4741 ccgcccgggc ggcgcgcgag cagtggaggc cggtgctggc gagcgtgggg ggcgaatcgt
     4801 gaacccctac cagttcctcg cccagcagcc cggtgtcggt gacgtgcgcg acctggtaca
     4861 gcagctcggc gcctggcacg actcgatggt ccttcaccag cgcaccgtcc ggcaggtcgg
     4921 cgccgaggag gcgtgcgacg agagctgcgc ccacgccgtc ggccgcgacc tctggatcca
     4981 ggcgaagatg cggctggggc cggtcgccga aacgttgacg ttcttgcgcc gctcggtgga
     5041 gcggaccacc cgggagtcgt gatcgccagg gcccaggggc ttgtggccag cgtgctgcgg
     5101 acgccctgcc tcagggccca aggaggagga tggccccgcc agggcgtgcg aactcgacga
     5161 ttatccgccc cggccgggcg tccaggttgc cgggccgcgc gtcgggcatg aaagacgggt
     5221 cctccgtcga gagcagcagt tcggctcccg accccgtcac cggcagctcg acgcgttcca
     5281 cgcccgcaag gcagacgagg gcggccagct tcaggtcgcc ctcgccggcc tccccccgcc
     5341 acaccccgac cgacgcctcg cccatcgcct cgacgcgcga gggcaccgac gcctgcagca
     5401 gcgggtggtg gccgcgcagt cccagcatgg cgcggtagaa gcgcagcacg ccggcgtgcg
     5461 gctcgacctg cgtctcctcc catcggagcc ggcttgcctc gaaggtcgcc tcgtcctgcg
     5521 ggtcggggac cgcgtcgcgc gccgcgggat cggcaaaggc ggcgaacccg ctgaactcgc
     5581 gccgccgtcc ctcggtgacc aggcgcccca gctcgggtcc gtgatccgtg aagtacagga
     5641 acggggtccc ggccgcccac tcctgcccca tgaacagcag cggcgtctcg ggcagcgcga
     5701 gcagcagcag cgatgcggcc aggtagacgg gcagatcgac ctggtggttc agccgcccgc
     5761 ccacggcgcg gttgcccacc tggtcgtggt tctggatgca gtagacgaag cgctcgaggg
     5821 gcacccccga ggggttggtg cctcgggcgc gcttgtggtg gcgcgagtac tgccccgtgt
     5881 aaaaccagcc ccgccgcgcc gtcgccgcga gatcgcgcgc cgtcccggtg tagtcggcgt
     5941 agtagccgtc cttgtcgccg gcggtcgcgc tgcgcacctg gtggtggaag tcgtcggccc
     6001 acaccgcgtc gaggccgtac cctctctcgg cttcgccgcg cacgagccgg tcgaggttcc
     6061 gatcgtcctc ggcaatcacg atcgcccggc gcccggccgc ccgcgcctcc tcccgcacgg
     6121 cggcgcccag ctcctcgagg aggtgccgcg ggctgcggtc ctcgagggtg tgggtcgcgt
     6181 cgaggcgaag gccatccagg tggaagtccc ggatccagta acgcgcgttc tcgaggatga
     6241 accagcgaac cagctcgctg ccgggcccgt cgagattgac ggcgcgcccc cacgggccct
     6301 cgcgctcctc cgagaagaac gacggcatga acgccgccag gtaggccccg tccgggccaa
     6361 ggtggttgta gaccacgtcg aggatgaccg cgatgccgag cgcgtgcgcc tcgttcacca
     6421 ggcgccgcag ccgttcgggg cgcccgtaac agcgggccgg tgcgaagagc gcgccggggt
     6481 cgtagcccca gttgcggttc ccggggaagt cggcgatcgg catcagctcg atcacgttga
     6541 cgccgaggtc cgccaggtag ggaagccggg aggcgacctc gtcgtaggtc cctgccctgg
     6601 agaaggtgcc cacgtgcagc tcgtagatgc gcaacgcccg cgggtcgggc ccccgccacg
     6661 cggcatcggt ccactcgaac cgcgggtcga tcacctctga cgggccgtgt actccccccg
     6721 gctggaagcg cgacccgggg tcgggcatcg ggaccatgtc gtcggcctgc agctggtagc
     6781 gagcgccggc gcccaggccg gggacgaacc cctcgaagag gccgtgcggc gccggctcga
     6841 gcgcgatcgg caggcctgcc ccctcgaggt ggatcagcag ccgccgggcg aagggggccc
     6901 ccacccggaa ggtcgtgcca tcgtcatgga ggagtgcgcc cggtgcgccg gggctcagcg
     6961 tcccggaagt gtccgccggc agacggcccc gcgtactccg gctcggggat ggagacatcg
     7021 ccagcgattc tgccaaatgc ctgccgaatg gacccctact ggcctttcag ccggcggagc
     7081 tcacggcggg cacgtttgtc gggtgcgcga gatgggcgcg ggccgaaact gcggcggaac
     7141 gccctggcca gctcgatttg ctccagctct tcaggagtcg gcggcggcgt ctgatcctcg
     7201 tacagtgccc gggctgccgc tttgggcacg tgcttctcgg ccagttcgcg aaccaccacg
     7261 acctgccgcc gcccgtgcgc ccgcgtaatc cggaggcggt cgcccacgtg gacgggccgg
     7321 tggggcttac cggcctgacc gttcacctcg accttgccgc cgctgcaggc ttgctgggcc
     7381 tcggagcgcg ttcgaaagag gcaggcgacg tcgagccaga cgtcgagcct gacgccggcg
     7441 gggctgggcg gtgtctgctc gctcaccgcc cgattttacc gcttgcttcc aatgggctcg
     7501 cttgtgttgg cgcagggctg aagccctgcg ctacgaccaa gcccaagccc cggccgagta
     7561 gggcgggcgt ccccgcctcg cccgaggccc gcagggccga agccgaggtg catgtttttg
     7621 cgacgaagcg gccgccccgg cgcatgccat cccgacgggc ggacgagctg cgtttcctgc
     7681 gggtcctcgg tagtctgtta cggaaaccgc agaaaaccga ggcatcctcc acagtcgcct
     7741 ggctgaggcc cgatggcccc gaccttgcta ccccacgtaa ccagccgcgc cgaatattcc
     7801 ctcaaaggtc aggagcaggt gacgggcatg ggttcatcgg cactgcgcat ccgcgagacg
     7861 ctggaacgca tccagggcga gttcagagag ctgccgggcc tgcggttgac ccttgcgcag
     7921 gcgcagcggt tgtggcgtct cgacaagaac gtttgtgatg cgttgctggc cgcgctggtc
     7981 gatgcccggt tccttgcccg caccgccgat ggcgcgttca ttcgtgggac ggctgtgaac
     8041 tgattcttcg cgcgacctgt cgttctcgct cgtgaagcag aaggaagaga acgacattac
     8101 ccaccgggtt ggcccggccg ccgtggaccg gcggtgaagg agccgacccg accaccttcc
     8161 gtggtttccc gggcggatgc ctttcggggc gtccgcctgc ggcacttcaa gaaagagact
     8221 caaggctcaa ggctcacggc gcaaggacgg ctcaagatac ttcctcagct tcctgaacgc
     8281 ctttccgcgg tggctgacct cctccttgcg gccggctggc acttcggcca gcgtctggcc
     8341 gaacggcggg tagtagaaga tcgggtcgta gccgaagccg ccggcgcctc gaggcggctc
     8401 gatgatccgt ccctccacgg ttcccgtggc ctcgaagaca atgcgcggga gggaagccgc
     8461 agccccttcg gggccggctg gcgcggcgag ggcgagcgcg cacacgaagc gggcgtcgct
     8521 gtcgacgcgc ccgctgcgtt ctcgaagcat tcggtagagc agctcgaact tctgaggata
     8581 ggacggcgcg ccaggcaggc cgaaccgcgc cgagtacacg ccggggacgc cgtcgagatc
     8641 gtcgatctcg aggcccgagt cctcggccac caccatccgg cccagcgccg acgcgtaggc
     8701 cagcgccttg gcgcgcgcgt tctcggcgaa ggtttggccg gtctcctccg gctcggggac
     8761 ggggtcgaga tcggccagcg agaggagtgt cagcccaagg ccgtcgagca cccgccggat
     8821 ctcgcggacc ttcccggcgt tcccggtggc aatcaccagt tcggcgatca tcgcctgaac
     8881 cggatgtcgc cgagcacctc gagctgcagg cgattgagcg tggcaattcc ctcggccgcc
     8941 aggtcgagca ttctcatcaa cgcgtcgcgt ccgaacgggg cggcctcggc cgtgccctgc
     9001 acctcgatga ggcggccgtc gcccgtctgc acgacgttca tgtccacttc ggcccgggag
     9061 tcttcctcgt aggcgaggtc gagcagcggc accccgtcca cgatgccgac gctggtggcc
     9121 gcgacgaggt cctgcagcgg cacggcggcg agctggccct gggcgtggag cttctgcagg
     9181 gcgagcgcca gggcgacgaa gcccccggtg atcgccgcgg tccgcgtgcc gccgtcggcc
     9241 tggatgacgt cgcagtccac ccagatcgtg cgctcgccga gctcctccat gcgggtcacc
     9301 gcgcgcagcg accgcccgat cagcctctgg atctcctgcg tccgcccgcc caccttgccc
     9361 gccgacgcct cgcgcacgct gcgcgtcgtg gtcgatcgcg gcagcatgcc gtactcggcc
     9421 gtcacccacc ccttgccgga accgcgccgg aacgggggca cgcggtcctc gacgctggcc
     9481 gtgcagacca cgcgggtgtc gccggcctcg atgaggaccg agccttcggc gtgcttcacg
     9541 aagtgcggcg tcaggcgggt cggcctgagc gtggacgggg tgcgctggtc ggatcgggtc
     9601 atcttcgaac tcgcggtctc accggggtgt gccctcgtcg atccagaagg tgctgcggac
     9661 gagggggcgg cgcaggtcta cgtggccggc cagcgtgtcg acctcgtggc cgtccacgag
     9721 gatctgcacg ccggtcagca cgggcaggtt cacggtcagc gaatcgacga ccgagtagac
     9781 cgtgaggatc tcgttcgtga ggcctccagg gtgggccgat cgcgcctcgg cgctcagatc
     9841 cacgaacgcc tggttggcct cggtcaggta gagagcacgg agccgggtcc ccgccgggat
     9901 cggcgacacc agcccctcgg gcgccggctc gagctgcgcc tcgacgatcc ggcgtgcctg
     9961 gtccgccgtg gtggcgccga acggcacttc ccgttccacg ggcaccaggt gcacaccgtc
    10021 ttccgccacg tagaacagcc gtgccttgat cttgcgcgcc tcgtctgccg acgcagccgc
    10081 gggcccgccc tcggagggcg gcgcttcgac cccggaggcc agccagcggc tcaagaccac
    10141 cgcgcccagc ccggcagcgg ccaccacggc ggccatcacg gcgatccaga cgatgagcga
    10201 gcgcctcacc gcgggggcct cgacgggttg ggagcggaga caccggttgg catcgcggcg
    10261 acgccgcgct cgcgcacctc gcggaaccgg atgaccgact gcagcagcgc ctggacgatc
    10321 aggtcctggt ggccggccga cttcagcagc cgctcgtcct cggtgttggt gagatacccg
    10381 gtctcgacga ccaccgccgg catgttggcc gccgccagca cacggaacgg cgcctggagg
    10441 accgggcgct cgttgacggc gatccggccg cgcagttcct cttcgaggat cgcggcgaag
    10501 gcggccgact cgtcgaggtg gcggacctgc gccaggtccc agagaatcag ctcgacgtcg
    10561 cgcgagccgc cgccgagggt cggcagcgcc ccgccagcga gggccgcacg gctcgcctcg
    10621 ccgccctgtc cggcgctcag gaagtacacc tgcgcgccgc gggtggcggg cctgagggag
    10681 gcgttcgcgt gcaggctgat gaagaggtcg gccttgttgg tgttggccag cgccgcgcgc
    10741 tcgtcgagcg acactgcctt gtcggcatcg cgcgtcatca gcacgcgcag gccgagccgt
    10801 ccctccagca ccgcccgcag gcgccgggcg accgcgagcg caacttcctt ctccagcgct
    10861 cccgacgggc cggaagcccc ctgctcgtcg ccgccgtgcc ccgcgtcgat gacgacggtc
    10921 ctgaccgagg ggcgcggcgg tgccaggttc acgagcggtt cggccgcggg cggcgacggg
    10981 ggcggcaccg ggatcgcctg gtcggtgaag gagggcacca gttcgacgat cacctcgctg
    11041 ttggcgtcgc gcgtcgcggc tgacgcgcgg taggagccga acctcgggcc gaggtccacg
    11101 gcaagcgtcg ccggggtatc gccgagccgg atcccctgaa gcaacggccc gggggtgacg
    11161 gcgggcagcg cgggatcgag ggccgtcgcg tcgaaccgca gcagcaggcg gttcccctcg
    11221 tgggtcacct ggtgccccgc acgcggcgca accgtcagcg tgagccggat caccccgccg
    11281 gcctcttgct ggcggacctc gacgcgcggc acgcggacgt cgccgaccag gacgaggcgt
    11341 gagccgcggc gcacctcgat gggcgtgccg gcaaccggcg cgatcgcccg cgaaaggaac
    11401 tcgatgggaa cgagccagcg gcggccgtca cggaccgggg cggccggcag cgacaccagg
    11461 cggccgtcgg cggaggcgag cggctgcccc ggcgtcagca gcacgcgctt cccgttggcg
    11521 gcggtcacgg tcaggcctcc gccgatgccg tcctcgcgca cggtcagccc gaagagcggc
    11581 gcgaggtcgt cgagcgccac catgagctgg tcgccagcct gcgaagcggg cagcggcgtg
    11641 cgcgcctgcg gcgagatgac cgtgaacgcc ggcgcgggac gcgcgccggc cgcctgcccg
    11701 gcaagcccgg ggtgcggaga cagccccgcc aaactggctg agagcaccgc aacgatcagc
    11761 agtctggatc gaggcaggaa caaggcgtac ccatgatatc agcccgcgtt cacccgctca
    11821 cggccttgct caacgcaggt gatgccggag taatgcggcc acaagggccg cccctgcggg
    11881 gcccgcgttg tcatatgctt gagcaatgag cctccgtctg atgcggccgg cgctggtctt
    11941 cctcgccatg ttcgtgaacc tgcccggcgt gcacgcacaa gggtggctgg ttcaaggcta
    12001 cccggcgggc gtgcaggtgg gcgcgggcgc cgtcgcgccg gtgggggagg cggccggcag
    12061 ctcgggcatc ggctggaacg tcggcggcgg cggagaactc gagttccgcc ccgggctcgc
    12121 gatccgggcc ggatacttct acgggcggtt tgcggccgag gacgccacgg ttgtcgcgga
    12181 atccgggagc ggcgcgctgc gcggcaagac gcagatgcac gccggcaccg tcgacctggt
    12241 ctggaagcgc cccctcaccg gccgcgaggc gaccgtctac ctgtttgccg gcccggtgat
    12301 cgcctaccgc cgggtcacgg tgaccagccc ctcgggagtt gccctcgccg agtctcccgt
    12361 cagcttctgc gagccgcact ggctgcagtg ttccccgacc tcgctcccgt tccaccgcct
    12421 gctcgggatc cgccgctcga ccgacgtcgg cgccagcgcc ggcgcgggcg tctcgttcga
    12481 cgtcggcctg agggcccggc tgttcgccga ggcgcgtttc gtcttcctcg acggcccgtc
    12541 gttccgcgac gcctccggct cgaagcgctc ggccgatgcc ttctacgtcc cggtcttcct
    12601 cggcgtgcgc ttccagtgac cgagcggggg gagccccgcg gcaggacccg cgaacctgca
    12661 tctccttgtc caatacacat gtggggtgga ctgggttccg ttcccgatat aatccccggc
    12721 cggaggactc atgttcgcta gacgctcgtg cccgtggtgg ggaattgcgc tgctcgtctt
    12781 cgtcgcgctc ggctcgcagg ctcttccgga ggccagccag gacgacccgg tcgtcgcgaa
    12841 gatcatccag ctcggcaaga ccgacaacca ggtcatggtg tggaacgact acgccagcaa
    12901 ccgcttcggc gggcgggaga ccgggaccaa cgcgtaccgc gacgccaccg agtgggcggt
    12961 gtggcagttc aggcagtggg ggttcgaggc cgagctcgac gaggccggag agctgcccgt
    13021 gggcttcaac cgcggcccgt ggttcggccg catgcggacg ccggtcgaga aggcgctccg
    13081 cttcggcacg ccctcgttca ccgcggggac caaggggatc cagcgcggcg gcgtagccat
    13141 cctgaaggcc gacccgttct cggtcccggg gcgcgcgcag ggcggcgtgc ccctgcccaa
    13201 ggagaccgtc gagaagaagc gcgcggcggt ggaggcggcg ctggccgaga tcgccgcgaa
    13261 caaggcgtcg tacaacggca agtgggtgtt gatcccgggc gccagcaccg ggttcgcgcg
    13321 cgacgggagg cgcggcacca agctgcccga cggcagcttc gagtacctcg acgccgagat
    13381 gatcccgccg gtgacgcgcg cgctggtcga ggccggggcg ctcggcacta tccagtcgtc
    13441 gaagccgccg gcgcccgcgc aggacggctt cctgccgccg ctgtccatcc tcgacggctt
    13501 cgtcccgtcg tgggacgcgc tcccggtgct gcccgacatc aagctgctcg acacgcagta
    13561 cgacgagatc aaggcgctcg tcgagaaggg acagccggtc gagctcgagt tcgacatccg
    13621 caaccacttc aagatgggcc ccctcaagta ccacaacgtc gtcgcgacgc tccgcggcac
    13681 cacctacccc gacgagtaca tcgtcatcgg cggccacctc gatgcgttca gcgtcgccac
    13741 cgggggcgtg gacgacgggt cggggttctc gccgaacatg gaggcgatgc ggctgctggc
    13801 gaaggcgggg gcgcggccca agcgaaccat cgtgtgcgtg ctcttcgcgg ccgaggagag
    13861 cgggttgctc gggtcgcagg cgtggctgcg gaaccggccg gggatcgagt cgaaaatcgt
    13921 catgatgatc aatcgcgacg gcagcccgag cgcgattacc ggcgcgagcg tgccgcccgc
    13981 ctggtacgac gacttcgcaa ggatcaccgc gccgctcgag acggcgaacc cgaggtggcc
    14041 cttcaagctg gtcaccaacg agtacccggc gccgaggccg gcctcccccg gcggcagcga
    14101 tcactcgtcg ttccagatgc gcggcgtgcc aacgctgggc gtgcagacct cgagcgtgct
    14161 cccggcggtg aacggggaga aggaacggaa cttcgtctat ccgtacgtgt ggcacacgac
    14221 caacgacctc tacgaccagc tggtgcccta cacggagcac cagcagcact cggcgctcgc
    14281 gaccgctgtg ctcgcctacg gcgtggcgaa cctcgacaag ccgctgccgc gggagggggt
    14341 cttcctgccg gacgggctgt acgccgacat cacgatcggc ggcggcgacg cgccgaagcg
    14401 cgtgatcgcg tcgctcgact tcgcgaacgc ccccctgcac gcggcggcct tcgtccggct
    14461 gttcgagggg aacaagccgg ggtcggcggc gcaggccgcg cgcccgccgg cggccgcgct
    14521 cgcgatgatg gggcagcggc ggccgccgga gatcgcgcct gtcgggcgga tcctcgacgt
    14581 gaggggcagg gtgaccggcg tgatcacctc cgagctgggg aggtcgatgg cggtgaggaa
    14641 cctgccgaag cgagccaacc gcgtgctgaa gcaggacgcc cccggcgtcc tggcgatggc
    14701 cgggcccagc acgttctacc tgaccagcgg gaggaagggg ggcggcggcg tggcgatcgg
    14761 ccaggtgatt gccggcgtca agacgttgcc caccgtgggc aaggacgacc cgatccgctc
    14821 ggtccgcatc ctccgcgtcg gccaggccgc ccgcgacttc aacacgagtg acgaggggtt
    14881 caaggagctg atgggaaagt aggggcggcc cttgtggccg ccccgacggc agacaatggg
    14941 gcggccttgc ggccgcccct ctcgcacaac gaatcaccat ccgacgtcca ggaccaccga
    15001 tccgcctctc actcccgcca gccgcagccc ctgaagcgcg ttgcgcacgc attccggcgc
    15061 tgggcgcacg gcgttggcgc ccgtcaccgc ctccaccctt tccaccgctc cgcctccgcc
    15121 gaagacaagc cgcagccgca gcgtgcccgt ctccggcgtg cagccggcag ccgacagcgc
    15181 gcgctgtacc gtgacggtaa tcgtctcgct tcggacgaaa cgtccggtga gacggttctc
    15241 caacagccgg acgcgcacac tcgcctgcgc ctgacgtggc ggcgacacaa ggtctggcgc
    15301 cgtcgagagc acggtgccgc ggccgcggtc ttcggcccgc ctcgggctcg ccagggagag
    15361 tgactcggcg gccacctgca tcttggccac gcccggcatc cctccaaccg cgaggtcgct
    15421 cacgccctcg ggcagcggca gcggctgctt caccgtctcg aggcgtccgt cctgacggcg
    15481 ccgccgctga tcgatcgcga caaacgacgt gtactccgtc agcagcccgt actcgagccc
    15541 cagtttcgtg atggccgcct ttctcgcggc ctcggccccc gtggcgcccg cctccgtccc
    15601 ggccccagta gcgccggcct ccgttccggc aaggtcgagc accgcgagcc gctcgcgcgc
    15661 ccacaggtag cggagcgcct cggcgccggt gacgagacgg ctgcggtcca ccttcacgcg
    15721 gcgctcgaac cttccctcgc ccgtaaagcc gctgacgacg atctcgccgg ccggctcgcc
    15781 gcggtacttg ccggcgagca cgagcggccg cgcggcgaag acgtcgggga tcttccccgg
    15841 ctcgaggtcg taggcgtcga agcctggcgt gcgcacgtcg atgccggtga gcaacggcga
    15901 ctcgatgtac tcgaggaacc gcgatgcggc gcgcggcgcc tcgccgggac caaggacgac
    15961 gaagggcagc ccttcgccgg cgcgtgcgag cccctcgacg aggtggcggt tcaccgagcc
    16021 gccgatcccg aacgcgaaca cgctcgcatc gtccaggcga tcgcggacga gtgcgaacgc
    16081 ctcgcgctcg acgttcacgt acccgtcggt cgcgatcacg atcgtgcggg ccacgtcgcg
    16141 gcgcgcccgg ggcaggtcga acgcccgccg aagggccggc agcagctccg tgccgccgcc
    16201 acccgtctgg cgctcgatct cctggagcgc gcgggccttg ttgacctcgg tcgccgggag
    16261 cgacgcgggc gagagcacgt aggaaccgcc cgagaagaag aggatgttga agctgtcgtc
    16321 gggctgcagg cggccgaaca ggttgcgcag cagcgccttg gtggtctcga gcgggaagcc
    16381 gtgcatcgag cccgacacgt ccacgatgaa gacgtactcg cgcggcggga ccagtgcggg
    16441 cgagaagcgg cgagggggct cgaccaggca gaggaagaag cgctcgccgc ccgactcgta
    16501 gagcatgagg ccggtctcga tgcggtcgtc ggcgagctgg tagcggatga ccaggtcctt
    16561 gttcgcggcc gccgcctcgt cgccgagcgt gagccgggcg cgcgtcgcgg aggggtagtc
    16621 aatcctcaac gcgtgcgacg gcgaggtgac gcgcttcaac gccatgccgc cgctgatgct
    16681 cgcgctgaag tcgaaccggg tccggtcggc ctcgccgctc cgcaggtagg ggttctgcac
    16741 ccagcgctcc ggctcggggg cggtggcggc gtcggacgtg gagtagcgcg gcccgacgac
    16801 cgtcgggtac acgaactcgt agacgcctcg ctccggcacg agcagctccg agtacaccag
    16861 ctcgacccgg atctcgtcgc cgggcaggat gttcgcgacg ttcatctgga agacgttggg
    16921 gcgctgctgc tcgaggagcg aggcactctg gccagactcc cgcgcctgct ggtactgccg
    16981 ccgcgcctcc tgccgttcct cgatgcgggc cgtgatcacc cgatcgccga tcgtcatctt
    17041 catcccgtgc accgccgccc gcgtggagcc ggggaagacg tagatggctt cgagcgcgtg
    17101 cttcccctcg ttccggtaga cctgcgtgac cttcacgcgc gcgatcacgc cggcgatgtc
    17161 cacctgggcc gaggtcgcct tcagcggcag ccggtcggtg gaggggtcgt cgccatgcac
    17221 gaagaagtag ggagagaggg tcctggtgtc ggggggatgt tcggtggcgg ccgcggcggg
    17281 ctgccacgac agcagcacga gcagcagggc gagcagtagt gtcggcatgt tcatctcccg
    17341 gcagcgtcat gctgcctctg gaggatgaac ggcgacgctg gagtttcttg caggcagcct
    17401 attcccgcca ggtcgccccg ccgtcgtcgg tggtgaagcg gcggccgtcg gaggcgtgga
    17461 cggtcgcccg gcggtcgctg tcggcgagga cggccaccag gtcgagccgc tcggggaagg
    17521 gaacggcgtg ccaggtccgg ccgtcggtgg tgcgcaggac gagcccccgg ccgcccacga
    17581 cccagcagac ttccggggac ggcgacgagc ctgcgacgag ccggccggct cccgtgtact
    17641 gcagctgcca cgtgcggcca gcgtcggcgg agccgaagat cctggcaccg ggatcgagtc
    17701 gccagaggag cggcctggcg ccggcgcctt tcacgatggc ggcctgcgag ccctcgcccc
    17761 gggccgtgga cacgacctgc tccggtgcgg cgagagccgc gtcggcggcg gccttggagc
    17821 cggtgggagc ggcgaccctg ggagcggccg cctcgccggc cttgaggcgt gttggtctca
    17881 cgccgagtgc gccgccggcc gcgcgcggct gggccgtgtg cgcctgcgcc tcggccatcg
    17941 cctcaggctc cgcggaagga gggggctgcg agggcggcgc ggccacaggg gcggcggcca
    18001 gctgcgggac cggggcaagc gtggcgctct gcccggcttc cgcctcgtcg gccctgaaag
    18061 gcgcggcttc cagcttcgcc tcgggcctgt ctgccacggc cttgtcccgc gttcgcgcca
    18121 gcacccgtgg cgtctcgttc accgcctcct tctcttcctc gttcccttcc tcgcgcttcg
    18181 cgcgcggcga agccgcctcg gcaagttgcg cagcactcgg aacggccttc ccggggagga
    18241 gctgggaggt gccgggcgcc tccggcggcg tgggtgactc cgcgggcgcg gtggcgacga
    18301 tcgagtcggg ggaaaggttc ggctggagaa cggccgcggg cttcagcgcc acgtagatga
    18361 ttgccgcgct ggcgagcgcg gtcgcgggga cgagccagcg gcgccagccg gcgagccacc
    18421 acgagggtgt ccgggccggc gcgttcgcgg gaatggcggc cggctcgggc atcgtggcca
    18481 tcatcgcgag cgcctgctgg cagcgctcgc acgaggcgag gtggggctcg agcgcctcgc
    18541 gctcggggcg ggcgagtgtc tgctcgacat aagccgacag ctcatccgcg gccgggcagg
    18601 catcagccga gcccgccggc ccgtcgctca tcgcggcgcg cagcagccgg tccatctcgg
    18661 catcccgatc gcggtcgcgt gccacttcac cctccatcat tcggaacgac gccgacgcct
    18721 ggtcttgcag gcccccttga gccttgagcc ctgagcccta ctccggcggc agcgccctcg
    18781 ccaggtcgaa ggctggatcc gtgcgtgcca gctcgaaggc gtcggccacc tgggtgtcgt
    18841 tcatcccgtg gcgctcgcgc agccggcgct cgacttcctg ccgcaggccg gtgcggcagc
    18901 gggccagctt tcgcgatacg gttgcctccg actcgccgag cacccggccg accgccgcca
    18961 gcttcagctc cctcgcgtag tagagggaga gccgcaggcg gtcgcgtgcg ggcagttgct
    19021 gcagcgcggc agccagcgcc gcgcgcaccg cgacgagccg tcgcgatcgg tccacctccg
    19081 gcggttgggc cggtaccgac ggttcgcccg gcgtgcccgg ctcggggagc ggctcgagcc
    19141 ggcgggacgc gcgcgcccgg tccaccgcgc gctgtgcgag gacggcacgc agccatccgt
    19201 tcagcgagca gcgcccgtgg aagtagtcga acagcgaccg gcggacgccg tcgcgctcct
    19261 cggtgccgaa caggtccgcg tacagcgagt cggccagctc gcggccggcg cctgggccgg
    19321 cggcggaagc ggccgccagc agtgccggcc tgaagtcgtg gacgaagtgt tcccacgccg
    19381 gctcgctgcc gcgtgcgcag gctcctgcca gtgcgaggtc ggcgaggtgc aatccgccga
    19441 ggtactcctc ggcctcgcgc tccgaaggcg gcgcctcgcg gaaccggtgc gcgaggcttc
    19501 gccgcagcga cgcctcgaag tcggcctcgg caaggcccca ccgcgcggca cctgcccggt
    19561 cgtagaggcg ccgaaccagc gctggcgcaa tctcggcggc ggcggtcgac gccctggaac
    19621 ctctcatcgg gaccgcccta ttctacgttc tctgacgccg gcagggacgc cggcgctact
    19681 ccgttcgggc ggcgctgccc gacggcgggc tgagttgacc gggcgcgagc cgccgggtca
    19741 acatggtgcg ccctcgtgtc gaatcgttcg aagtccatcc catcgggaca ggaaccatgc
    19801 tcaaccgacg tcttcttctt cccgccctgt tgatcctgac gcttgcgggc gccgttgggt
    19861 cggcgcaggg cccggctcac ggcaccgcga agcgggcacc gggacggacc tcggcggacg
    19921 tcctcacgcg gcccgagctg accggctacc gcgagacgag ccgctacgac gacgtgatgg
    19981 cgttcatgga agcgatggcg gcggcctcgc cgcagatcca tctcaccacc tacggctaca
    20041 cctacgaggg gcggtcgctg ccgctcgccg tcatcggcgc gccggcggcc acgcccgagg
    20101 cggtgcgcgc caccgggaag acgcgcatct acatccaggg gaacattcac gcgggcgaag
    20161 tggaagggaa ggaggccgcg ctctggctgc tgcgctcgat cgcgaagggc gaacgggccg
    20221 cctggttccg gtcggtcgtc ctgctgatca acccgatcta caacgccgac ggcaacgaaa
    20281 aggtgaacct tcgcaaccgt ggccgccagt acggcccgat cgggggcatg gggcagcgct
    20341 acaacgccca gaatctcgac ctcaaccgcg actgcaccaa gctcgagacg cccgaggggc
    20401 gctcgcttgc gaagctgctg accgactacg accctcacgt cgcggtggac ctccacacga
    20461 ccaacggcag ccctcacggc tactacctga cgtaccagac ctcggtcagc ccgaacacgt
    20521 cgccagggtt gaccgcgctg gcgcgcggcc gcctcctgcc gttcatcacc gcgcaggtga
    20581 aggcgaagca cggctgggac tacttttact acggcaacct ctcgcgccag ggggagcgtg
    20641 cctggtacaa cgacctcgat ctctacaagc cgcgctacac gcagacctac tttggcctcc
    20701 gcaaccgggt cggcctcctc gtcgagacct actcctacgc gagcttcgag gaccgcatca
    20761 agtcgaacta ctggttcctc gaggagctgg tgaacttcgc cgccgccaac ggcgaggcca
    20821 tccgctcggc gaccgcggcg gccgacgccg agtcaatcgt cggcaggcaa caggcggtgc
    20881 gcggcaggct cgtgaaggac cccgacccgg tcccgatcgt gctcgccgac tcggtggacg
    20941 agcggaaccc gtacgtgccc gaccggccca tgctgcgccg cgtggccggt accgagcgcg
    21001 tcgaaacgat gccgcactac gcgacggtgg aggctgccga gaccagcctt gcgccgcgag
    21061 cgtacctggt cccggccgtt ccgccgccgg caggcgagaa agccgactcc gggcggcccc
    21121 cgtttcccgg cggcccgcca gccgcgggac gagggcccca gccggcctct cccttccgcg
    21181 acaaccagca gatgctcgcg cggatcgtgg accggttgac agcgcacggc gtccgcttcc
    21241 acaggctcga gtcggagagg acggtggctg ccgagcagtt tcgaatcgaa gcgtccacgg
    21301 tgcaggagca gagctggcag aacatgaaga tccgcgagat caccgggagc tgggaggcca
    21361 ccggcgcgaa gctgccggcc ggctcgctcg tcgtgccgat ggatcagccg ctggcccgcc
    21421 tggtgttcgt gctgctggac gcgcgctccg acgacgggct catggcatgg gggctgctgg
    21481 acgacgtgct ggcaaggacg cccgagccgg ccgtgtaccc cgtgctgagg accatgaccg
    21541 cggtgccgga gtaagatcgt gttcgggacg tcccgaacac gacacgagga gggcgcgatg
    21601 aacgtcgatc acatccactg gctcgggcac gcgacgtttc ggctcgagga cggcggatcg
    21661 cagatctact tcgatccctg gaagctgccg gccggggccc cgcgggcgga cgtcatcctc
    21721 atcacgcatg cccactacga ccattactcg gaagacgaca tcgcccgcat cgagcagcca
    21781 ggcacgctct tcgtcgcgcc gcccgacgtc gcggcgaagc tgaagggccg caaggtgacg
    21841 agcgccgagc cgggcggcac ctaccgggtc ggcccggtga ccgtggaggc aacgccggcc
    21901 tacaacctgg acaaggacta ccacaagaag agggagcact gggtcggcta catcgtgacg
    21961 ctctcgagcg gtcagcgcat ctaccactcg ggcgacaccg atgccatccc cgagatggac
    22021 gacgtcgagg ccgacgtggc gttgatgccc tgcggcggca cctacacgat gaccgccgcc
    22081 gagatggccg aggcggcgaa ccgcttcaag ccctccgtgc tcatccccat gcactggggc
    22141 gacatcgtcg ggcagaaatc cgacgcggaa gccgtggcca aggcgttcaa gggccgcacg
    22201 gttatcaagc aagttgagaa gtaggggcga ccttgtggtc gcccttcggg gcccttgtgg
    22261 ccgcccgctg gaattacgct cccgcctcca gcaccttctc gatcaggcgc gactcgggta
    22321 ccgccccctc gaccctgatc gtctcgttga tcaccgtcag cgggacggct tgcacctggt
    22381 agcggttcgc gaggtggggg aattcggtcg cctccaccat gtcggccgac acgagatcgc
    22441 tggcgagcgc cagtttgtgg gcgagcacca cggcccgcgg gcagtacggt caggttggcg
    22501 tgacaaacac ctgcaggtgc accggcttgg tgaggcgcgt caatgcctgc cgggtggcgt
    22561 cggcgagctt cacgtcgccg gtgcccacca tgcggagccc ttcgacgagc gagctgaact
    22621 cgtatccggc gggaatcccg tacagccgca cgccgtggtg cgtcgcccca ccgtcggcca
    22681 gcaggacgac cgcggggatc ttgtccacgc cgtactcctg cgcgcgcgcc tcgtcggcct
    22741 ggaagtcgag cacctccagc gacagcttct ccgactgctg cgaaacctcc tcgaccagct
    22801 cgcgcgtttc gctgcagtag tcgcactcga gcgcgccccc ttctccctgg gtgaacatca
    22861 cgatcttgac gggtcgctcg atcgcggcca gcgcgtcccg cacctgcttg cgaaccttgt
    22921 cgtccagcag cggcacgtcg cccccctttc gtcggtgttg cggcaggccc gcctccattc
    22981 tacgcgcact gccgccgcag gaattgacga gctcgttaca gcctcgtgat ccgcttgacg
    23041 gaattgcgcg gacgttcgcc gacctcttcc gatcactccg gaacgaatct gttgatcgcc
    23101 ggccggacca caagatgcac cggggtcacg gacggtcgca gcccggctct cggcatgcga
    23161 ccgctcccga cgctcctggc gcctgccatc tgcgcttccc gggaggccgt cggagcaacc
    23221 cggttggggc ctcccgagag cagcttgcga atccgcacat caagaggaaa caggacgatg
    23281 gcatcttcgt cgcgaccctt cgtactgacg ctattgatcg cggtccttct cgtccccgcc
    23341 tgggccggcg cgcagaccgt gaccaacgcg cgcatcgtgg agttcgttcc ctcacccgat
    23401 cactcggaac ggttgtcgga cggccgcgcg gtggtccagc ggtacgtgat ggaggtctac
    23461 caggccggcg ccagtcagcc gttccacacg gttgacatgg gtaagccgtc accgcagtcg
    23521 gacggcaaga tccggttcga cttctcggcg accgtggcgg cgtggccggc gcccggcggc
    23581 gagtaccagg cgagggtggc agccgtcggt ccgggcggcg aaggagtgag cgatccctcg
    23641 aaccgcttca ccgtgagcag ctgctcctac acgctctcgc gctcgtcggc gctgttcccg
    23701 gcggggggcg gcggcggtga ggtggcggtc accaccacct cgggctgcgc ctggacggcc
    23761 agcgagggtc tctcgtgggt gacgatgatg acggcgggcg ccaccggctc gggccgcgcg
    23821 accttcagcg tcgccgccaa cagcggcacc agcgcgcggg ccgggacgat tacggtggcg
    23881 gggcaagcct tccaggtgac ccaggagggc agctgcagct actcgctgtc gccctcgacg
    23941 gcctcgttcg cagcctcagg cggtacggga tcgatcagca tcaccgccgg gtcgggctgc
    24001 gcatggacgg ccgcggcgag cgcctcgtgg gtctcggtca gcacggccag cgggagcggc
    24061 gcagggacgg tccgctacac cgtggcggcc aacacctcca ccagcagccg cagcgccacg
    24121 gtgacggtcg cggggcgggt gcacacgatc tcgcagtcgg ccgcgagcgg tagcggcggc
    24181 gggacgccgg ccacgagcct tccctcgccg tggacgaacg gcgacgtcgg gtcggtgggc
    24241 ctctccgggt cggcgagcta ctcgtctggc gtcttcacgg tcgtcggggc cggcgccgac
    24301 atctggggca gcgccgacag cttccacttc gtgcgccgat cgctggccgg cgacgccgat
    24361 ttcgtggccc gcgtcaccag cgtccagaac accaactcgc ttgccaaggc cggcatcatg
    24421 ctgcgggcga cgacggcgtc gaacagccag cacgtgatcc tcgacgtcaa gccgaacggc
    24481 ggcgtggagt tcatgacgcg cacgagcacg ggcggctcga ccgcgtacgt ggcggggacc
    24541 acccagggct tcccggcctg gctccgcctc gcgcgccgcg gcagcaccat caccgcgtcc
    24601 gtctcgtcgg acgggcgcac gtggcggacg gtcggctcgc gcagcctctc gctgccctcg
    24661 acggtgcagg ccggcctggt ggtgaacagc cacaacgcgt cggtccgcaa cacgtcgcgc
    24721 ttcgaccagg tgtcggttgc agccgcctcg gccgaagcgc cggcggcgtc cacggggctg
    24781 ccgtcaccgt ggcagaccct cgacatcggg agcgtcgggc gcgcggggtc ggcaacctac
    24841 tcgtcgggat tgttcaccgt ccgcggtgcg ggcgccgaca tctggggcac cgccgacgcg
    24901 ttccggttcg tgtaccggcc gcagctgtcg ggcggcgaga tcgtggcgcg cgtcacctac
    24961 gtgcagggca cccacagcct cgccaaggcc ggcatcatcc tccgcgacgg aaccggcgcc
    25021 aacgcccggc acgtgacgct gtcggtgaag ccgaatggcg gggtggagtt cctccggagg
    25081 gcgtccacct cgggtagcac cgcatacctc gggggcacga cgcaggcatt tccggtgtgg
    25141 gtgcggctgc gccgcagcag ctcaacggtg accgccgccg tctcggcaga cggcacgacg
    25201 tggcgcacgg tgggcagcac cacgatctcc acgaccacgc cgctcatggg gctcatcgtc
    25261 agcagccacg acacgggggt gctgaacctc tcgaagttcg atcgcgtcac cgtgaggtag
    25321 cggacaatgg agaacccccg gccaccgcgc ggtgaccggg ggttcttctg agcgccgagc
    25381 tgggaacgaa cgctagcccc tgatctcctt caccagctcc gcggccttgt acaccttctc
    25441 cagggcctcg aggatggcgc gcgagtcaac cgagacggca cggttgatcg actcctcgta
    25501 gatgaagtag ccgggcagca tctgcaggtt gccgtcgaag atcaggccga ccacctcgcc
    25561 cttccggttc accatcgccg atccgctgtt gccgccgacg atgtcgttgg tggtgacgag
    25621 gttgaagggg gtcttgaggt tcaacgccgc cttcgcctcg gcccagcgcg gggccaggtc
    25681 gtacggcggc tcgttgccgt gctcggcggc ccgctggtag agaccggcga tcgtcgtgta
    25741 cggcgtcacc ttcttgccgt tctccatgta gcccttcacc gtgccgtacg acagcctcag
    25801 cgtcccggtc gcgtcggggt aggcgctctg gccgtacacg acgaacaccg cctggccgat
    25861 cttcggatag gcggcggcct gcaccgtcgt gacctgctct tcgtacttct tggcgatcgc
    25921 ctgggcgtcg ggctcgagcg agcgcgccag cacgatgaac ggatcggtgg agacgtcgat
    25981 cgcgccgcgg cccccggccc gcagcaggtt gcggatctcg ggatcggtca ggcgggtctt
    26041 gtccaccagt tcggccgctc tggccgccgg cgtggcgccg ccgaggattc gcttggtgat
    26101 ggcgtggtcc acacccagct gctcgaccat gaacgcgagc gactcggtga ggttcagctt
    26161 ctcacgctcg aggttgatct gcggctcccc gccaccgggc cggcccgctg cggcggggcc
    26221 gcccccacgc gggccgcgcg cctcctcggc ggcgttggcc ggcgaatagg cttgcctgac
    26281 g
//
LOCUS       DAOPQX010000010        24805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2546_length_24805_cov_4.723152, whole genome shotgun sequence.
ACCESSION   DAOPQX010000010 DAOPQX010000000
VERSION     DAOPQX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 24805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24805
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2546_length_24805_cov_4.723152"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1011
                     /locus_tag="PKK95_01190"
     CDS             109..1011
                     /locus_tag="PKK95_01190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOC16846.1"
                     /translation="MQQPDPYKSILEALRDGVYVVDRNRLITYWNPGAAALSGYTAEE
                     VVGRFCFDNILQHVDAGGNNLCRNGCPLAATIADGQPREVEVYLRHRDGQRVAVKVRA
                     MPLRDGSGRVTGAVEVFSENESALAARRRQVDLERAALSDPLTLVANRRFAEGTLASN
                     LGEVKRLGWRLGIILADVDLFKEVNDTYGHVTGDRALRTIAATMAGAVRIYDLVARWG
                     GEEFMVICPGIDASDLLRLANRVRTLVSRSGLRLDDGQSLSLSISIGTAVATEDDTVE
                     SLLARADAALYRSKAEGRNRVTMG"
     gene            complement(1028..2128)
                     /locus_tag="PKK95_01195"
     CDS             complement(1028..2128)
                     /locus_tag="PKK95_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC16847.1"
                     /translation="MLRCLSIGLVLVFVFLTAPAAAQPGAANPPYVQEEVQVTSGAIR
                     LAGTFTLPAGAGPHPAVVLLTGSGPQSRDEEVLGMPVFRLLADHLTRAGVAVLRCDDR
                     GVGGSTGSVAQSTSSDFADDALAQVAWLKTRKEIDARRIGLLGHSEGGIVAPMAANRS
                     RDVSFVVLLSGPAQTGEQIMLAQNELVGRASGVPEEQIRKNGALQRRMFAAARSGEGL
                     EALTAEVRALASEALGRLPEAQRKAIGDLDAAAARAAEQQVAGLKTPWFRYFLTYDPV
                     PALEKLACPALAIFAEHDTQVPAGPNREAMEAAFERGGHRDHTIVVVPRANHLYQESA
                     TGAPTEYAMLKKEFAPGFAETITSWILNGRNR"
     gene            complement(2215..3108)
                     /locus_tag="PKK95_01200"
     CDS             complement(2215..3108)
                     /locus_tag="PKK95_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HOC16848.1"
                     /translation="MLALKLGPPPLAALVPAIVLVLGSYGAYIRAHLRARPLAAPGAP
                     TTAAATLSTADPSLTLPLTSLAVALAGGLAAVAYAWVSYPDLPPQVPTHFGPSGRPDA
                     WSPRSFSSVMLLPLTTLLLGTAMAVMACLMTRAKRAIRHPDGGISLAAQQRFRQVSAN
                     YLALVVIVMTAMLALMSIFAIRTGLGLAAGMPPALMALVAVLLVVAVGGGLYIALRYG
                     QGGARLERPAASAPLTNGLADNSRWVLGIFYVNRDDPSIFVEKRFGIGYTINFGNPKA
                     IALVVAFLAIVAYIVVAGVTI"
     gene            complement(3108..3239)
                     /locus_tag="PKK95_01205"
     CDS             complement(3108..3239)
                     /locus_tag="PKK95_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16849.1"
                     /translation="MSETTVLALIIVFEVLLVGGMLLIQPFVVRRGLVFGVYVGEAA"
     gene            complement(3276..3665)
                     /locus_tag="PKK95_01210"
     CDS             complement(3276..3665)
                     /locus_tag="PKK95_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC16850.1"
                     /translation="MVAPLVVVLDRDAGEAAYEQIASQIREAVATGTLVPGSPLPGVR
                     TLATDLGVNFNTVARAYRVLEEQGFVIIRDRSGAEVATPAARASRQARAMLERELWEV
                     MARMRQAGFSERDLRKAAESCLDRLKE"
     gene            3844..4554
                     /locus_tag="PKK95_01215"
     CDS             3844..4554
                     /locus_tag="PKK95_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC16851.1"
                     /translation="MPGTPVFSVRGLTKVYDMGEVQVPALRGIDLDLEEGLLTVVLGH
                     SGSGKSTLLNIMGGLDVPSSGRVFYRGEDLTAADDRQLTEYRRRRVGFVFQFYNLIPS
                     LTARENVDLVAEIADDPMPSEEALGVMGLRERLDHFPSQLSGGEQQRVAIARAVAKRP
                     DVLLCDEPTGALDISTGVVVLEALARVNAELGATVTVITHNAAIAGMAHRVVTLGDGR
                     IVEDRVNESRLSPREIRW"
     gene            4551..6944
                     /locus_tag="PKK95_01220"
     CDS             4551..6944
                     /locus_tag="PKK95_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOC16852.1"
                     /translation="MRRAGGGRRRWPALDRKLARDLRAAAGQVAAISAVIGAGIAVLV
                     AMFSTFDSLDLSLRTYYEEYRFGDVFVSAVRAPLSAAERVAAIPGVAQVEARVVASVT
                     IDVPGMSDPGTGRLVSVPGDRRPVLCDLFLREGRYLEAGRPDEVLASELFARAHGLGP
                     GDTVGAIVNGRRRELRIVGLALSPEFIYQIRPGELFPDNERFGVFWMERRALAAAFQM
                     EGGFNDLVIGLTRGASEPDVIAEVDRVLRPYGGTGAIPRALQLSHWYLQSELEGLRTS
                     GIFVPAVFLGVAAFLLNVVLSRMVAVQRSQIAVIKALGYGNAAIGAHYVKWSLMVALS
                     GSAVGAVGGALLGRALTGLYTGFFQFPILLYRVDSRVLLAAVLVGVAAAVAGAIGAVS
                     RAVRLPPAEAMRPEPPARYAISWAERAGLRALLSPAARSVLRTVQRHPGRAALSVVGI
                     GLGASLLVVANFSLDAIDVMLDLQFNQAQRYDLAVSFVEPTSARALDEVARLPGVLRA
                     EPFRVVPARIRAGPRSRVLAITGVRARAELSRVVDASSRVVDLAPGGLVLSDKLARVL
                     GVGVGTRVMVEVLEGERPVREVPLVGLVSEYTGMNAYMDLESLHRLMREGGTLSGAYL
                     AVDPARRDALYRQLKATPRVSGVLNKRAAMQSFLDTVASMMSQMQAIFVAFAAIIAFG
                     VVYNNARISLGERNRELATLRVIGFTRAEVARVLLGELVVVTGLAAPFGLAGGYGLAA
                     ALVWAYDTEMYRLPLVIGGGTYAFAGGTIVAATVLSAAVVRRRLDRLDLVAVLKTRE"
     gene            6948..8174
                     /locus_tag="PKK95_01225"
     CDS             6948..8174
                     /locus_tag="PKK95_01225"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOC16853.1"
                     /translation="MNDARRRRVGFWGGLTLLLVVLLAFALWPRRVTVDTAVVDEGPL
                     RVTLEHEGRTRVRDRYVVSAPVAGRVQRIELEPGDPVAAGKTVVATFLPAVPPLLDVR
                     ASAEGAARVQAAEAALARARAERERARTEAGFAAVERDRARRLEEAGVISAQALEAAE
                     TEAAARARALAAAEAAVNGAAHELEVARAALITPAAPASGSRTGQALALRAPADGVVL
                     RRLRESEAVVPQGEPLLEIADTSSLEVIADFLSADAVRIEPGQKVSIEQWGGPAPLNG
                     RVRRIEPSGFLKVSALGVEEQRVWVVIDFDDPRSAWQALGDGYRVEARVVVWERARVV
                     RAPSGSLFRDGDGWAVFTVEDGRAARRDVQVGQRGGTTAEILAGLRSGEVVIVHPPDA
                     VGDGARVAARAAGGSR"
     gene            8273..9532
                     /locus_tag="PKK95_01230"
     CDS             8273..9532
                     /locus_tag="PKK95_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOC16854.1"
                     /translation="MNQLQQLMPREGAEILLVLFLSFLVGLEREEHKGEGDYSFGGVR
                     TFPLIGLIGYATAMLSGGQIAPVAVGFAVVSAFMLVAYRHKIGQSSLAGATSEMSALV
                     TYLIGALVRTGHFWVATTLGVVSLLLLELKVVLESMARRAEPAEILTFAKFLLLSAVI
                     LPALPNQPLGPFGINPFRTWLVVVAVSGLSYASYVFQRATRGRGGIVLGALLGGAYSS
                     TVMTVVLARRAGGENRPHLFSGAMLVASGTMFLRLIALLALFNGELASRLAPSFLGLA
                     AAAIVGGLFLARIPDPRASVPARQFVARNPLELRAALLFGALFVVMLAATDLAVTRLG
                     SVGVYVLGAVMGVADVDPFIMSMTQAARQATPLGVAAVAVLVTVASNNLVKGVYAFLF
                     ADRRTGTLGLASLAALAAATLLPIAFF"
     gene            9540..10409
                     /locus_tag="PKK95_01235"
     CDS             9540..10409
                     /locus_tag="PKK95_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOC16855.1"
                     /translation="MKLPPFIAEHLERIDRELDRLVPPKDQAPPVLHRAMRYTLLAPS
                     KRVRAVLTLLSAEVCGGGAEAALPAAAAIELVHASSLILDDLPALDDAALRRGRPANH
                     VAHGEAMALMAAFNLFDLAFATIARSYDAPAAQRLTALLAGAVGSEGLIAGEVEDLQA
                     AAGTISFETLERIHRWKTGALFVAAAVAGAVTAGGRPDQSAALSAYAKNLGLAFQIVD
                     DLLDVEGDPAETGKARLADARKTTFVSFSGVEGARQLARELCDTAVAALAPFGARASR
                     LRQLADFVAARRS"
     gene            10443..12392
                     /locus_tag="PKK95_01240"
     CDS             10443..12392
                     /locus_tag="PKK95_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC16856.1"
                     /translation="MANVDDLRARLRDLGYLDAGVDRFVLGAASGGRGLLAVAGRASA
                     RVGVLAALLLGPSAAIALAARLPGLVTGVRDGVVLTLYLSALFGVAVALFAFGVSVLL
                     GSLAARTGGERMAARATLLARAAGVLVGAGCLAYLVLWWRAVNPEGSIRLAGARTAVV
                     LAWAAFVSLLLGHAAHVTTLALIARGSGPAPARRGPFRRRWTARAALGIAAFAGAAML
                     LWTTTRGEGAGSAPPPSSLGRAPTGVRLTVLAIDGVDLSLVERLEAQGKVPVLARMVT
                     GPRLRLEPSDAPDPARTWTSLATGMSPQVHGVGGIEARRVSGIGGRAALRDSGLAGSI
                     AAATDLLRLTRPALITGMERRVKTFWEVAAEAGLSTAVVNWWATWPAEGTGTVLSDRA
                     VLRLERGGALDAEIAPASLYPRLQGVWPRLRQEAAREVELGFPVEGEAAAALRRAALQ
                     DALAVRLTLLLAAGEPGSERFDLEAVYLPGLDIAQYELLVRGGSGLPPSALAARVEAV
                     ERYHVFLDRLLKPLAERPDRPVAFITDPGRSPRASDASGSPPREPRPGVLALHGGPFR
                     GTTASDGERADVVPTLLYALGIPISRELPGRPAFALFDPAFVEAHPPRQVDTYGRREV
                     APRPAGSSPLDREMLDRLRSLGYVR"
     gene            complement(12420..13862)
                     /locus_tag="PKK95_01245"
     CDS             complement(12420..13862)
                     /locus_tag="PKK95_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOC16857.1"
                     /translation="MQPADGSEARVYLPDTNVLVNDPDSLFKLGRGNVVVIPYPVVRE
                     LDHLKDALNGTGAAARRVVHLLDDYQSGREPSRLVEGIPLPESGLLIFDDNVLSEVDA
                     WDGFDHRSNDDRIILLARKWKRLRPGAHVSVVTRDVAMRVKARPLGVLAEDYRDDKRI
                     SSLDQLYSGAATIEVPEACSSLLTDLHRETRVEASRVLEVAPPGGLLPNQCCTIRTAE
                     TGKAAHAIYKAGSPPYFRVVRKPESERGITPVNAEQAFAHALLSDPEISLVTLAGSAG
                     SGKTLMALLAAYQQLREGYARLIIYRPNIEIGQSLGFLPGTLDEKFAPWTRPVIDNLE
                     LILRGLTRGGGIVSLEEAGRAGGRAGRKEEPPRAVADLQELVAREIIEIAPISYLRGR
                     SIHEAFIVADEAQNMNHHEVKTLLTRSGQGTKVVFTGDPDQVDVPDSSAVTNGLIQLV
                     ERFKGQQEFGHLTMTTTVRSRLAALAASLL"
     gene            14151..15422
                     /locus_tag="PKK95_01250"
     CDS             14151..15422
                     /locus_tag="PKK95_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16858.1"
                     /translation="MKPFSFSRLLKVSGLVALALVLGAGVSLAQSAPQSVGTTGQSAQ
                     AAGDQYRTATRLGGSTALICQIRNVQQLKREMSRPRTRARVAGVLDQAGVSKAAADEV
                     ISILSSGDANQLRDQPFQVGDTMAWMGTQSRGKANIIRNVRWGGRRPFPAWQFDVDDG
                     ETLYHFVLPKACGNLALKSSERSPKAIAEEARKAEEARKAQEEAARKAEEARKAEEAR
                     KAEEARKAEEARRAEEARKAEEARKAEEARLAAEAEAARLEADRRDRIDFFVDGAFGK
                     ERRLREVALAAPVAGALVAERGVCAPLLGIKAGPDLRIAPGWRIAPALGVAINFDETD
                     NTSLFAEAEINRWFGRKGFIGSGIGVWDFTHSDTVAPVWLLQGGGQLWKGTGANESEL
                     HLVAQGRLFLDKMDDIENNYQFWGGLRYIFR"
     gene            15623..16519
                     /locus_tag="PKK95_01255"
     CDS             15623..16519
                     /locus_tag="PKK95_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16859.1"
                     /translation="MFRGLTTLVVAGTLGVLAAGDVRAQSEPQSTTGSGAQSSASQEP
                     QGQQSQQAAAARQRLIEARESLAEVAKMPEAAELQGQGRTEVSQAISNFNALLTAESD
                     WYDQYKLVMQNLFNVLGPPEAGESAAGSSAGTSGSSTAGSSATGTAGEEVPPALRSKL
                     VEFRQKLMAFGREAGAPDVTGGVSSTVGAGSSEPSSTSGTSGTAGSPPSAQSGTPAAE
                     GESLEQHIDAIDDIIQQALAGTPGSGSSTAGTSGTTESTAGAAGATGTTPGVVTIDRE
                     KLDRIQSHLQRMREIARSKGIK"
     gene            16586..18268
                     /gene="ettA"
                     /locus_tag="PKK95_01260"
     CDS             16586..18268
                     /gene="ettA"
                     /locus_tag="PKK95_01260"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOC16860.1"
                     /translation="MASYQYIFTMKGLTKVHPPDRKVLEDIWLAFLPGAKIGVLGANG
                     AGKSTLLRIMAGLDRDFAGEAFAADGVSVGFLPQEPELDPSKTVLGNIEEGVAETRAL
                     LAEFDRLSERLAGDLSPDEMDATLDAQGRLQDRIEAAGAWDLDSRLQLAMEALRVPPP
                     DADVQSLSGGERRRVALCRLLLRSPDLLLLDEPTNHLDAESVAWLERFLHDYPGTVVA
                     VTHDRYFLDNVAGWILELDRGRGIPWQGNYSSWLEQKEQRLEREEKQEARRQRILARE
                     LEWIRMSPRARQAKGKARLNAYEALLAQDAAPRIERGEIYINPGPRLGDVVVEASGLR
                     KGYGDRLLIDNLSFTLPRGGIVGVIGPNGAGKTTLFRLITGQEPPDAGTLRIGETVRI
                     GYVDQSRDAMQADRTVWEEISGGLDEVPIGKRVVSSRAYASWFNFKGRGQQRPVGSLS
                     GGERNRVHLAKLLRGGCNLLLLDEPTNDLDVDTLRSLEDALVEFAGCAVVVSHDRWFL
                     DRIATHILAFEGDSQVVWFQGNYQDYEADRRRRLGAEAERPHRLKFRKLVRS"
     gene            18347..18514
                     /locus_tag="PKK95_01265"
     CDS             18347..18514
                     /locus_tag="PKK95_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16861.1"
                     /translation="MPKRISPATHAAIDILLVGALLLGGSLLWHCYRRLRGPRTRRWR
                     LRGWEPLGIGA"
     gene            complement(18678..19154)
                     /gene="msrB"
                     /locus_tag="PKK95_01270"
     CDS             complement(18678..19154)
                     /gene="msrB"
                     /locus_tag="PKK95_01270"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOC16862.1"
                     /translation="MPTRKSASGYDLTPLDGETIHRLAARLSPEERQIVLHQGTEPPF
                     CGTLLDNKRRGSYVCRLCGLPLFRSNSKFDSGSGWPSFYEPFDPEHVVELVDRSHGMV
                     RTELRCARCDAHLGHVFPDGPRPTGLRYCINSASVEFTEADPVPDRLHVPDAEPAS"
     gene            19303..21951
                     /locus_tag="PKK95_01275"
     CDS             19303..21951
                     /locus_tag="PKK95_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOC16863.1"
                     /translation="MILPSLAALFFLLQSSAAAPSQPDLSPGTRYDPKVPTLQQAVGH
                     DVGERISSPEEIAAYLKALHGADPARSRLVEYARSWEGRPLHVLIVGSAERLAKLDQV
                     KADLRRLADPRQLPAADGDRLVRELPVVTWLLHSVHGDETTSSDAALAEAYHLLAARG
                     SEAVDTILRESIVLIDPLQNPDGRARFLSHNLQAAAARPDPDPLAAEHDEPWPGGRSN
                     HYLFDMNRDWLALSQPETRGRVALALEWFPHVVADLHEMGGDSTYYFAPPAEPANPYI
                     TAKQFEWLNVLGRENARRFDERGFAYFVREVFDSFYPGYGESWPIFHGAIGMTFEQAS
                     PSGLLFRRDDDTVLSYRQGIVQHFTAAITSAETAARNREQILRDFLEYRRSAVQEGER
                     GPVREYLIPPGADRARHDRLAELLTLHGLEVRRAGEPVKAGSRTLPAGTLVLPLAQPA
                     SRLLRNVIEPTIQMDEKFLKEQERRRQKRLGDQIYDMTAWSLPLLFDLEIVTLPQPSG
                     AKASVVTPRPATPGGPLPAAKVGYLLPWGSGTAAVVAEALQAGIRVRAAGDAFTQAGR
                     KYPIGTAIVRISENGPDLASRLGPIVARHMAEAVPLETSWVEEGISLGSDRVVALKAP
                     RVAMAWDVPTSSASAGWARYVLERRFGQPATAIRTAALRNADLTRYDVIVLPAGDYTQ
                     ALGEDGVRRLKEWARMGGTIVTLGEASRWATREKVALLETTTELRGGKPDVEPAGDEK
                     PKKVAAGEPAKPFDLQQAIQPDRDPPDAVPGAILRVSLDTEHWLSAGLDDEIQVVVEG
                     RRIFTPIKLDKGRNVGLYAARDRLLAAGFAWDNPMEQLPQKAFLLHQPLGRGHVIAFA
                     EDPNYRAFAGCTELLFMNAVLLGPAH"
     gene            complement(21961..22767)
                     /locus_tag="PKK95_01280"
     CDS             complement(21961..22767)
                     /locus_tag="PKK95_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HOC16864.1"
                     /translation="MVYELSDVTCRYQDVTALRGLSLSIEAGSRVALVGANGSGKSTL
                     LRLLDGLLFPDAGLVRYRGTPLTEAAFAPDGFAYSFRRQVGLVFQDADAQLFNPTVFD
                     EVAFGPLQLGWGRDELHARVGETMEKLRVAHLRDRVPQRLSTGEKKRVALASVLVLDP
                     DVLLLDEPAAGLDPRSESEVIDLLVGWAGGAKTVVTATHDLGALEDIADRCCVLNDGR
                     LVADAAPAVILADLPLLQRANLVHAHRHTHPGRQHSHPHLHGHEHARDGA"
     gene            complement(22767..23591)
                     /gene="cbiQ"
                     /locus_tag="PKK95_01285"
     CDS             complement(22767..23591)
                     /gene="cbiQ"
                     /locus_tag="PKK95_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOC16865.1"
                     /translation="MTRRRGYVERTLDGLLEVMDRAREAERRAGTRGLLQQLDPRVKL
                     AGVLALVAAVALARNLATLAFLLLLAVVAASASRLPLRLLVTRLWLGTLAFTGIIALP
                     ALFTTPGQEWFTLPGAGLIVTVQGATSAAYLLLRVLGAATLAFLLVFTTPWTHVLKAL
                     RTFRVPAVAVVVLGMTWRYILVLLQAAHAMFDARRSRTVGPLPGPVRRRTTAAAAGVL
                     LERSMRLSQDVFLAMQSRGFRGEVHVADQFRMRPRDWAALGAFAVLATLCVWMGRQ"
     gene            complement(23598..24680)
                     /gene="cbiM"
                     /locus_tag="PKK95_01290"
     CDS             complement(23598..24680)
                     /gene="cbiM"
                     /locus_tag="PKK95_01290"
                     /inference="COORDINATES: protein motif:HMM:NF008873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HOC16866.1"
                     /translation="MHIPDGYLSPLTSAALYAGAAPFWYVALRRVRAALHTRLVPLLS
                     LFAAFSFVVMMFNIPLPGGTTGHATGVALAAVVLGPWASVLAISVALAIQAFFFGDGG
                     ITTLGANCFNMAVAGSLAAWAVYRAVAGNAPLSSPRRAMAAGLAGYVAVNLAALLTAI
                     EFGLQPMFFADASGAPLYAPYPLRIAVPAMMLGHLTIAGLAELVVSAGVVAFIQRSDP
                     SLLARGAGMTAPAAPGNGARGGSLRPLWAGLGILMVLAPLGILAAGTAWGEWAAEDFN
                     DPAARQEIADASLGVAPPAAAPSGLDRLSAVWTAPWPDYAPPFVRSARFGYVLSAVLG
                     VGLVILAFLLLERLGRPGRREGQASG"
BASE COUNT         3521 a         8671 c         8990 g         3623 t
ORIGIN      
        1 gcctcgtgct cttcagccat acgcctcccg ttcctgctgc acagggaatg ctctcgcggg
       61 ttctcggccg taaaggagta cgatattaca ctacgagccg ggggggtcgt gcagcagccg
      121 gatccgtaca agagcatcct cgaggcgttg cgtgacgggg tctacgtcgt ggaccgcaac
      181 cgcctcatca cctactggaa tccgggagcc gcggcgctca gcgggtacac ggccgaggag
      241 gtcgtcgggc gcttctgttt cgacaacatc ctccagcacg tggacgcggg cggcaacaac
      301 ctctgccgca acgggtgccc gctggccgcg acgatcgcgg acggtcagcc gcgcgaggtc
      361 gaggtctacc tgcggcacag ggacgggcag cgcgtggcgg tcaaggttcg cgccatgccg
      421 ctgcgcgacg ggagcggccg cgtcacgggc gcggtggagg tcttctcgga gaacgaaagc
      481 gccctggccg cccggcgtcg ccaggtcgac ctcgaacgcg ccgcgctcag cgacccgctc
      541 acgctggtgg ccaaccggcg gttcgcggag gggacgctgg cctcgaacct cggcgaggtg
      601 aagcgcctcg gctggcggct cggaatcatc ctcgccgacg tcgacctctt caaggaggtc
      661 aacgacacct acgggcacgt caccggcgac cgggcgctga ggacgatcgc cgcgacgatg
      721 gcgggcgccg tgcggatcta cgacctggtc gcgcggtggg gcggggaaga gttcatggtg
      781 atctgccccg gcatcgacgc gtccgacctg ttgcgcctgg ccaaccgcgt gcgcacgctc
      841 gtgagccgct cggggcttcg cctcgacgac ggccagtcgc tgtcgctgag catctcgatc
      901 ggcaccgccg tcgccaccga ggacgacacc gtcgagtcgc tgctcgcccg cgccgacgcc
      961 gccctgtacc gcagcaaggc ggaggggagg aacagggtga cgatggggtg aggacgccga
     1021 agtacaacta ccggttcctg ccgttcagga tccagctcgt gatcgtctcc gcgaagcccg
     1081 gcgcgaattc cttcttcagc atcgcgtact cggtcggggc ccctgttgcc gactcctggt
     1141 agaggtggtt ggcgcgcggc acgaccacga tcgtgtggtc acggtgcccg cccctctcga
     1201 aggccgcctc catcgcttcc cggttcggcc cggccggcac ctgcgtgtcg tgctcggcga
     1261 agatggcgag cgccgggcac gccagcttct cgagcgccgg gacgggatcg taggtgagga
     1321 agtaccggaa ccacggcgtc ttgagacctg cgacctgttg ttcggccgcg cgggcagcgg
     1381 cggcgtcgag gtcgccgatt gcctttcgct gcgcctcggg cagccgtccc agcgcttcgc
     1441 tggccagcgc gcgcacctcg gccgtcaggg cctccaagcc ttctcccgac cgcgccgccg
     1501 cgaacatcct gcgctgcagc gcgccgttct tgcggatttg ttcctctggc acgccgctgg
     1561 cgcggccaac cagctcgttc tgcgccagca tgatctgctc gccggtctgc gcggggccgg
     1621 acagcagcac gacaaaggag acgtcgcgcg accggttcgc cgccattggc gccacgatgc
     1681 cgccctcgct gtgcccgagc aggccgatgc gccgggcgtc gatctccttt cgcgtcttca
     1741 gccacgccac ctgcgcgagc gcatcgtcgg cgaagtcgga ggaggtcgac tgggcgacgc
     1801 tgccggtcga cccgcccacg ccgcggtcgt cgcaccgcag cacggcaacg ccggcgcggg
     1861 tcaggtggtc ggccagaagg cggaacaccg gcatccccag cacctcttcg tcccggcttt
     1921 gcggcccgct cccggtgagc agcacgacgg ccggatgcgg ccccgcgcca gccggcagcg
     1981 tgaaggtgcc ggccagccgg atcgcgccgc tcgtcacctg cacttcttcc tgaacgtagg
     2041 gcgggtttgc ggcgccgggc tgcgcggccg ccggcgcggt gaggaagacg aagacgagga
     2101 cgagaccgat cgagagacat cgcagcatgt gaacctcaca cggttgtcag tcagtgtccg
     2161 ctgtcacaac ggctcgttcc cctggtcggc aggcgggttc gcttgggcgc tcggtcatat
     2221 cgtgacgccg gccaccacga tgtaggcgac gatcgcgagg aaggcgacga ccagcgcgat
     2281 tgccttcggg ttgccgaaat tgatcgtgta gccgatcccg aagcgcttct ccacgaagat
     2341 cgacgggtcg tcgcggttca cgtagaagat gccgagcacc cagcgcgagt tgtcggcgag
     2401 cccgttggtg agcggggccg aagcggccgg gcgctcgagg cgcgcaccac cttgcccgta
     2461 ccgcagcgcg atgtagagcc ctcctcccac cgcaaccacc aggagcacgg caacgagcgc
     2521 catcaacgcg ggcggcatcc ccgccgcgag gcccagcccc gtcctgatcg cgaagatcga
     2581 catgagcgcc agcatggccg tcatgacgat cacgaccagc gccaggtagt tggcgctcac
     2641 ctgccggaag cgctgctgcg cggccagcga gatgcctccg tcggggtgac ggatcgcccg
     2701 cttcgcccgg gtcatcaggc acgccatgac cgccatcgcc gtccccagga gcagcgtcgt
     2761 cagcggcagc agcatgaccg acgagaacga gcgcggcgac caggcatcgg gccggcctga
     2821 cggaccgaaa tgggtcggta cctgcggcgg caggtccggg tagctgaccc aggcgtacgc
     2881 gacggcggcc aggccgccag ccagcgcgac ggcaagtgac gtcagcggca gcgtcagcga
     2941 gggatcggcg gtcgagagcg tggcggcggc ggtcgttggc gcgcccgggg ccgcgagcgg
     3001 cctcgcccgc aagtgggcgc ggatgtaggc gccgtaggac ccgagcacca ggacgatcgc
     3061 cggcaccagc gctgccagcg gcggcgggcc cagcttcagc gcgagcatca ggccgcctcg
     3121 cccacataca cgccgaacac cagcccccgc cggacgacga acggctggat gagcagcatg
     3181 cccccgacga gcagcacctc gaacacgatg atgagggcta ggacggtggt ctcggacata
     3241 gggatccttg ccgggatacg ggccgcctcg tgtcgttact ccttcaggcg atcgaggcag
     3301 ctctccgccg ccttgcgcag gtcgcgctcc gagaagccgg cctgccgcat gcgcgccatc
     3361 acttcccaca gctcccgctc gagcatcgcg cgggcctggc gtgaagcccg cgcggccggc
     3421 gtggccacct ccgcgcccga ccggtcacgg atgatgacga acccctgctc ctccagcacg
     3481 cggtacgcgc gggccacggt gttgaagttc acgccgaggt cggtggccag ggtccggacg
     3541 ccgggaagcg gcgagccggg caccagcgtc cctgtcgcca ccgcctcgcg gatctgcgag
     3601 gcgatctgct cgtaggccgc ttcgcccgcg tcgcgatcca gcaccacgac caacggcgcc
     3661 accatgtgct gaactgtata tcaattggta gacagaatca agggaggcct gcatgcagct
     3721 ccggacggga gtgcagacgt gggaggttgc gccaaccgtg agccggtgac gcgccgtgga
     3781 aaggcgccgc gcgcctgcgg ttcgtccggg caggagcagg cccaggcgta gactatcggg
     3841 gagatgccgg gcactccagt cttctcggtc cgcggcctga caaaggtcta cgacatgggc
     3901 gaggtgcagg tcccggcgct ccgcgggatc gacctcgacc tcgaggaggg gcttctcacc
     3961 gtcgtcctcg gccattcggg cagcgggaag tcgacgctgc tgaacatcat gggtggcctc
     4021 gacgttccca gctcgggccg cgtgttctac cgcggcgagg atctgacggc cgccgacgac
     4081 cggcagctga ccgagtaccg gcgccggcgc gtcggcttcg tgttccagtt ctacaacctc
     4141 attcccagcc tgacggcgcg cgagaacgtg gacctcgtgg ccgagatcgc cgacgacccg
     4201 atgccgtcgg aggaggccct cggagtgatg ggcctgcggg aacggctcga ccactttccg
     4261 tcgcagttgt cgggcggcga gcagcagcgg gtcgcgatcg cccgggcggt ggccaagcgc
     4321 cccgacgtcc tcttgtgcga cgagccgacc ggcgccctcg acatctccac cggggtggtc
     4381 gtgctggagg cactggcgcg agtcaacgcg gagctgggcg cgacggtgac ggtcatcacg
     4441 cacaacgccg cgatcgcggg gatggctcac cgcgtcgtca cgctcggtga cggccggatc
     4501 gtcgaggacc gggtgaacga gtcgcggctc tcgccgcggg agatccgctg gtgaggcggg
     4561 cggggggcgg ccgacgccgc tggccggcac tcgaccgcaa gctcgcgcgc gatttgcgag
     4621 ccgcggccgg acaggttgcg gccatcagcg cggtgatcgg ggcgggcatc gccgtcctgg
     4681 tggccatgtt ctcgaccttc gattccctcg acctctcgct gcggacctac tacgaggagt
     4741 accgcttcgg cgacgtcttc gtgtcggctg tgcgggcgcc cctgtcggcg gccgagcgcg
     4801 tggccgcgat tcccggcgtc gcccaggtag aggcgcgggt ggtcgccagc gtgacgatcg
     4861 acgtccccgg catgtccgac ccgggcaccg ggcggctcgt ctcggtgccg ggcgatcggc
     4921 ggcctgtgct gtgcgacctg ttcctccgcg agggccgcta cctggaagcc ggccgtcccg
     4981 acgaggtgct ggcgagcgag ctcttcgcgc gggctcacgg gctggggcca ggggacacgg
     5041 tgggagcgat cgtcaacggc cgccgccgcg agctgcgcat cgtcgggctc gcgctgtcgc
     5101 cggagttcat ctaccagatc cggccgggcg agctgttccc cgacaacgag cgtttcggcg
     5161 tcttctggat ggagcggcgc gcgctggcgg ccgcgttcca gatggagggg ggcttcaacg
     5221 acctcgtgat cggcttgacg cggggggcat ccgagccgga tgtgattgcc gaggtcgacc
     5281 gtgtcctgcg gccgtacggc ggaacgggcg cgatcccgcg agcgcttcag ctgtcgcact
     5341 ggtacctgca gagcgagctc gaggggctgc ggacgtcggg catcttcgtg cccgccgtgt
     5401 ttctgggcgt tgccgccttc ctcctgaacg tggtcctctc gcggatggtc gcggtgcagc
     5461 gctcgcagat tgcggtgatc aaggctcttg gctacggcaa cgcggcgatc ggcgcgcact
     5521 acgtcaagtg gagcctgatg gtcgcgctgt ctggctcggc ggtcggtgcg gtcggcgggg
     5581 cgctgctggg acgcgcgctg actggcctct acaccggctt ctttcagttc ccgatcctgc
     5641 tgtatcgggt cgactcgcgc gtgctgctgg ccgcggtcct ggtgggcgtg gcggcggcgg
     5701 tggccggcgc catcggcgcg gtgagccgcg cggtgcgcct cccgccggca gaggccatgc
     5761 ggcccgagcc gccggcgcgt tacgcgatca gctgggccga gcgcgccggc ctcagggcgc
     5821 tcctgtcgcc cgccgcgcga agcgtcctcc gcacggtgca gcggcatccc ggccgggccg
     5881 cgctgtcggt ggtcggcatc ggccttggag cgtcgctgct ggtcgtcgcg aacttctcgc
     5941 tcgacgccat cgacgtgatg ctcgatttgc agttcaacca ggcgcagcgg tacgacctgg
     6001 cggtctcgtt cgtcgagccc acctcggctc gcgcactcga cgaggtggcg cgtctgcccg
     6061 gcgtcctgcg ggccgagccg ttccgcgtgg ttcccgcgcg aatccgcgcg ggcccgcgat
     6121 cgcgcgtgct cgcgatcacc ggggtgaggg cgcgggcgga gttgagccgg gtggtggacg
     6181 cgtcctcccg cgtcgtggac ctcgccccag gggggctcgt cctctccgac aagctcgccc
     6241 gcgtgctggg cgtcggcgtt ggcacgcggg tgatggtgga ggtgctggag ggagagcggc
     6301 cggtgcgcga ggtgcccctc gtggggctgg tttccgagta cacggggatg aacgcctaca
     6361 tggacctcga gagcctgcac cggctgatgc gggagggtgg gacgctgtcg ggggcctacc
     6421 tggcggtgga tcccgctcgc cgcgatgcgc tctaccgcca gctcaaggcc acgccgcgcg
     6481 tcagcggcgt cctgaacaag cgggccgcga tgcagagctt cctcgacacc gtggcgagca
     6541 tgatgtccca gatgcaggca atcttcgtgg cgttcgccgc gatcatcgcg ttcggggtgg
     6601 tctacaacaa tgcgaggatt tcactcggcg agcggaaccg cgaactggcg acgctgcgcg
     6661 tgattggctt cacgcgcgcc gaggtggctc gcgtgctgct cggtgagctg gtggtcgtga
     6721 ccgggctggc ggcccccttc ggccttgccg gcggctatgg ccttgcggcg gcgctggtgt
     6781 gggcgtacga caccgagatg taccggctgc ccctggtgat cggcggtggc acgtacgcct
     6841 ttgccggagg cacgatcgtc gcggcgaccg tcctgtcggc ggcggtcgtc cggagacgcc
     6901 tcgaccgcct cgacctggtg gcggtgctga agacgcggga gtagggcatg aacgacgcgc
     6961 ggcggcgacg ggtcgggttc tggggcggct tgacgcttct cctcgtcgtg ctcctggcct
     7021 ttgcgctgtg gccccgacgg gtgacggtgg acactgccgt ggttgacgaa ggacccctgc
     7081 gcgtgacgct cgaacacgag ggccgcacac gcgtccgcga ccggtacgtg gtgtcggccc
     7141 cggtggcggg ccgcgtccag cgcatcgagc tcgagcccgg cgacccggtt gcggccggca
     7201 agacggtggt cgcgacgttt ctgcccgccg tgccgccgct gctcgacgtc cgcgcgagcg
     7261 ctgaaggcgc cgcgcgcgtg caggcggcgg aggcggcgct ggcgcgggcc cgcgccgagc
     7321 gcgagcgggc ccggacggag gccgggttcg cggcagtcga gcgggaccgg gcccggcggc
     7381 tggaggaggc aggcgtcatc tcggcccagg cgctggaggc ggccgaaacg gaggcggcgg
     7441 cacgcgcacg cgcgctcgct gccgccgaag cggcggtgaa cggtgcggcc cacgaactcg
     7501 aggttgcccg cgccgcgctg atcacgccgg cggccccggc atcgggaagc cgcacggggc
     7561 aggccctcgc gctccgcgcg ccggcggacg gcgtcgtcct gcggcggctg cgcgagagcg
     7621 aggcggttgt gccgcagggc gagcccctcc tcgagatcgc ggacacctcg tcgctcgagg
     7681 tcatcgccga cttcctgtcg gccgacgccg tgcggatcga gccgggccag aaggtgtcga
     7741 tcgagcagtg gggcgggccg gcgcccctca acgggcgcgt ccggcgcatc gagccctcgg
     7801 gcttcctgaa ggtgtccgcc ctcggtgtcg aggagcaacg ggtgtgggtg gtcatcgact
     7861 tcgacgaccc tcgatcggcg tggcaggcgc tcggcgacgg atatcgtgtc gaggcccggg
     7921 tcgtcgtctg ggagcgggcg cgggtcgttc gggcgccgtc gggcagcctg ttccgtgacg
     7981 gggatggatg ggccgtgttc accgtcgagg acggccgcgc ggcgcggcgc gacgtgcaag
     8041 tcggccagcg cggcggcacc accgccgaga tcctcgcggg actccggtcg ggagaggtgg
     8101 tgatcgtcca cccgcccgat gccgtcggtg acggcgcgcg cgtcgccgcg cgggcggccg
     8161 gaggttcgcg gtaacatgcg ccgacgccgc gtaccttgag gcggcggccg gcacgcgtcc
     8221 ggcggtgccc cgcccgccgt cagccggcga catctgtccg gcgaggtgcc cgttgaacca
     8281 gctccagcaa ctgatgcccc gggagggcgc cgagatcctg ctcgtgctct tcctgtcgtt
     8341 cctggtcggc ctcgagcgcg aggagcacaa gggcgagggc gactactcgt tcggcggtgt
     8401 ccgcaccttt ccgctgatcg gcctgatcgg ctacgcgacg gcgatgctgt cgggcggcca
     8461 gatcgccccg gtggcggtcg ggttcgcggt ggtctcggcc ttcatgctgg tcgcctaccg
     8521 gcacaagatc gggcagtcgt cgctcgccgg cgccacctcg gagatgtcgg cgctggtgac
     8581 gtacttgata ggagcgctgg tccggaccgg tcacttctgg gtggcgacca cgctgggcgt
     8641 cgtcagcctg ctgctgctcg agctgaaggt cgtgctcgag tcgatggccc ggcgggccga
     8701 gccggccgag atcctgacgt tcgcaaagtt cctgctgttg agcgccgtga tcctgccggc
     8761 cctgccaaac cagcccctcg gcccgttcgg gatcaacccc ttccggacct ggctcgtggt
     8821 ggtggcggtg agcggcctct cgtacgccag ctacgtgttt caacgggcca cccgcggccg
     8881 cggcggcatc gtgctcggcg cgctgctcgg cggggcctac tcgtcgacgg tgatgacggt
     8941 ggtgctggca cggcgagcgg gcggcgaaaa tcgcccgcac ctgttctcgg gcgcgatgct
     9001 ggtcgcgtcg ggcacgatgt tccttcggct gatcgcgctg ctggcgctct tcaacggcga
     9061 gctggcctcg cggcttgcgc cctcgttcct cggccttgcc gcggcggcca tcgtcggcgg
     9121 attgttcctg gcccggatcc ccgacccgcg ggcgagcgtc ccagcgaggc agttcgtggc
     9181 acgcaacccg ctcgagctga gggccgcgct cctgttcggc gcgctcttcg tggtcatgct
     9241 cgcggccacc gacctggccg tcacgcgcct gggcagcgtt ggcgtgtacg tcctcggggc
     9301 cgtgatgggc gtcgccgacg tggacccgtt catcatgagc atgacgcagg cggccaggca
     9361 ggcgaccccg ctgggcgtgg ctgccgtcgc cgtgctcgtc acggtcgcga gcaacaacct
     9421 ggtcaagggg gtgtacgcgt tcctcttcgc cgatcgccgg accggcacgc tcggcctggc
     9481 gtcgcttgcg gcgctcgctg ccgcgaccct gctgccgatt gcattcttct aaggctggaa
     9541 tgaaactccc tccattcatt gccgaacacc tcgagcggat tgaccgcgag ctggatcggc
     9601 tcgtgccgcc caaggaccag gcgccgccgg tcttgcaccg cgccatgcga tacacgctgc
     9661 tcgcgccgtc gaagcgggtg cgggcggtgc tcacgctgct ctcggccgag gtgtgcggcg
     9721 gcggcgccga ggccgcgctc ccggccgcgg ccgcgatcga gctggtgcac gcctcgtcac
     9781 tgatcctcga cgaccttccc gcgctcgacg acgcggcgct tcggcggggc cggccggcca
     9841 accacgtggc gcacggggag gccatggcgc tgatggccgc cttcaacctg ttcgacctcg
     9901 cgttcgcgac gatcgcccga tcgtacgacg cgccggcagc gcagcggctc accgccctcc
     9961 tggcgggtgc cgtcgggtcg gaagggttga tcgccggcga ggtggaggac ctgcaggcgg
    10021 cggcagggac gatctcgttc gagaccctgg agcggattca ccgctggaag acgggtgcgc
    10081 tgttcgtggc ggccgccgtc gccggcgccg tgacggccgg ggggcgtcct gaccagtcgg
    10141 ccgcactgtc ggcctacgcg aagaacctcg ggctcgcgtt ccagatcgtg gacgacctgc
    10201 tcgacgtcga gggcgacccg gccgagacgg ggaaggcgcg gcttgccgac gcccgcaaga
    10261 cgaccttcgt ctcgttcagc ggcgtggagg gagcgcgcca gctggcgcgg gagctgtgcg
    10321 acaccgccgt ggccgcgctc gcgcccttcg gcgcccgcgc ctctcgtctt cgccagctcg
    10381 ccgacttcgt cgcggcacgt cggagctagc ccccagcccc cagcccctat tacgattacc
    10441 ccgtggccaa cgtcgacgac ctgcgcgccc gtctccgcga cctcggctac ctcgacgccg
    10501 gggtggaccg gttcgtgctc ggggcggcca gtggggggcg gggcctgttg gccgtcgccg
    10561 gccgcgcgag cgcgcgggtc ggcgtcctgg cggcgctgct gctcggcccc tcggcggcga
    10621 tcgcgctcgc cgcccgcctg ccgggcctcg tcaccggggt ccgggacggg gtcgtgctga
    10681 cgctgtacct gtcggcgctg ttcggcgtcg cggtggcgct cttcgcattc ggcgtgagcg
    10741 tgctgctcgg gagcctggcg gctcggaccg gcggcgagcg catggccgct cgcgcgacgc
    10801 tgctcgcccg cgcggccggc gtgctcgtgg gcgcgggctg ccttgcctac ctggtgctct
    10861 ggtggcgtgc cgtgaacccc gagggcagca tccggcttgc cggcgcccgc acggcggtcg
    10921 tgctcgcctg ggcggccttc gtttccctct tgctgggaca cgccgcccac gtcacgacgc
    10981 tcgctctgat cgcgcgggga agtggcccgg caccggcgag gcgcggcccc ttccgccggc
    11041 ggtggaccgc gcgcgccgcg ctcgggatcg ccgcgtttgc cggcgccgcg atgttgttgt
    11101 ggacgaccac gcgcggcgag ggcgccggtt ccgcgccgcc cccatcgtcg ctcggccgtg
    11161 cccccacggg tgttcggctg accgtcctgg cgatcgatgg cgttgacctg agcctcgtgg
    11221 agcgactcga agcgcagggg aaggtgccgg tgcttgcgcg gatggtgacc ggcccccggc
    11281 tgcgtctcga gccgtccgac gcgccagatc ccgcgcgcac ctggacctcg ctggcgaccg
    11341 ggatgtctcc gcaggttcac ggcgtgggcg gcatcgaggc ccggcgggtg agcgggatcg
    11401 gaggacgcgc ggcgctgcgc gactccgggc ttgccggatc gatcgcggcg gcgaccgacc
    11461 tgctgcgcct cacccgcccc gcgctcatca ccgggatgga gcgccgggtg aagacgttct
    11521 gggaggtggc cgcggaggcc ggcctttcaa ccgcggtcgt gaactggtgg gcaacctggc
    11581 ctgcggaggg aaccggaacc gtcttgagcg accgggccgt gttgcgcctc gagcggggcg
    11641 gagccctcga cgccgagatc gcacccgcca gcctctatcc gcggttgcag ggggtctggc
    11701 cgcggttgcg gcaggaggct gcccgggaag tcgaactcgg gtttcccgtc gaaggcgaag
    11761 cggccgcggc gctccggcgc gcggcgctgc aggacgccct cgcggtcagg ctgacgctgc
    11821 tcctcgccgc cggcgagccg gggagcgagc gcttcgacct cgaggccgtt tacctgccgg
    11881 gactcgacat tgcgcaatac gagctgctcg tgcgcggcgg cagcggcctc cccccgtcgg
    11941 cgttggccgc ccgcgtcgag gcggtcgaac ggtaccacgt cttcctcgac cgcctgctga
    12001 agccgctcgc tgaacgaccc gaccgcccgg tcgccttcat caccgacccc ggacgctcgc
    12061 cgcgcgcgag cgatgcgagc ggttccccgc cgcgggagcc gcgtcccggc gtcctcgcgt
    12121 tgcacggcgg gccgttccgg ggaacgacgg cgagcgacgg tgagcgcgcc gacgtcgtgc
    12181 cgacgctcct ctacgcgctg ggaatcccaa tcagccgcga gctgcccggc cgtccggcgt
    12241 tcgcgctctt cgatcccgca ttcgtggagg cgcacccgcc gcggcaggtc gacacctacg
    12301 gtcgccgcga ggtcgcgcct cggcccgccg gcagctcacc cctcgaccgg gagatgctcg
    12361 accgcctcag gagccttgga tatgtgaggt aggggcggcc cttgtggccg ccctcacacc
    12421 tagagaaggc ttgcggcaag cgccgccagg cgcgagcgca ccgtcgtggt catcgtcagg
    12481 tggccgaact cctgctgtcc cttgaagcgc tcgacgagct ggatgaggcc gttggtgacg
    12541 gcgctcgagt cgggcacgtc cacctggtcg gggtcgccgg tgaacaccac cttggtgccc
    12601 tggccgctgc gcgtcagcag cgtcttcacc tcgtggtgat tcatgttctg cgcctcgtcg
    12661 gcgacgatga aggcctcgtg gatgctgcgg ccgcgcaggt aggagatcgg cgcaatctcg
    12721 atgatctcgc gcgccaccag ctcctgcagg tcggcgaccg cacgtggcgg ctcctccttc
    12781 cgccccgccc ggccgccggc ccggcccgcc tcctccagcg agacgatgcc ccccccgcgc
    12841 gtgaggccgc gcaggatcag ctcgaggttg tcgatgacgg ggcgggtcca cggtgcgaac
    12901 ttctcgtcga gcgtcccggg gaggaacccg agcgactggc cgatctcgat gttcggcctg
    12961 tagatgatca gcctcgcgta accctcgcga agctgctggt aggcggcgag cagggccatc
    13021 agcgtcttgc ccgacccggc gctgccggcg agggtgacca gcgagatctc cgggtcggac
    13081 aacagcgcgt gagcgaaggc ctgctcggcg ttcaccggtg tgatcccgcg ctccgactcc
    13141 ggcttcctca ccaccctgaa gtacggcggg cttcccgcct tgtagatcgc gtgggccgcc
    13201 ttgcccgttt cggccgtgcg gatcgtgcag cactggttcg gcagcaagcc tccgggcggc
    13261 gcgacctcca ggacgcgtga cgcctcgacg cgggtttcgc ggtggaggtc ggtcaacagc
    13321 gacgagcacg cctcgggaac ctcgatcgtc gccgctcccg agtacagctg gtcgagcgac
    13381 gagatcctct tgtcgtcgcg gtagtcctcg gccagcacgc cgaggggccg cgccttcacc
    13441 cgcattgcga cgtctcgcgt gacgacgctg acgtgagccc cgggacgcag ccgcttccat
    13501 ttcctcgcga gcaggatgat ccggtcgtcg ttgctgcggt ggtcgaaccc gtcccacgcg
    13561 tcgacctcgc tgaggacgtt gtcgtcgaag atcagcaggc ccgactcggg aagcgggatt
    13621 ccctcgacca ggcgtgatgg ctcgcgcccc gactggtagt cgtccagcag gtgcaccacg
    13681 cgccgcgcgg ccgcgcccgt cccgttcagc gcgtccttca agtgatccaa ctcccgcacg
    13741 accgggtacg ggatcacgac cacgttgccg cggccgagct tgaacagcga gtccggatcg
    13801 ttgacgagca cgttggtgtc gggaaggtaa acacgggcct cggatccgtc ggcaggctgc
    13861 atcgatccac tcccaaggga cacacgccga gtctgcggca gtatcgcacg gatcgccgca
    13921 cgcaccgtgg tgccgtgctt ctggaacgga tctcgcttgt tccggccgag tcaggccgtc
    13981 gcgcggcgac gccgcgtgca aggctgcctc gtcgagcgcg aggaggagcc ggccactgcc
    14041 gtcgcaggac cgtgcaggaa agtgcgggcc agccggctga aatgcgcgat ctgccgcttg
    14101 tcccgtagta ttatggctgg gatggtttct ctccctacaa cggagcgatc atgaaaccct
    14161 tcagcttctc tcgtctgctc aaggtgtcag ggttggtagc cctcgcgctc gttctgggcg
    14221 ctggcgtctc cctggcgcag tcggctcccc agtcggtcgg gacgaccggg cagtcggcgc
    14281 aggccgcggg cgaccagtac cggacagcca cgcggctcgg cggatcgacc gcgttgatct
    14341 gccagattcg gaacgtgcag cagctcaagc gcgagatgtc gcggccgcgg acgcgcgcgc
    14401 gcgtcgccgg tgttctcgac caggcgggcg tcagcaaggc ggccgccgac gaggtcatct
    14461 caatcctctc gtcgggcgac gcgaaccagc ttcgcgacca gcccttccag gtcggcgaca
    14521 cgatggcctg gatgggcacg cagtcacgcg gcaaagccaa catcatccgg aacgtccggt
    14581 ggggcggccg gcggccgttc cccgcgtggc agttcgacgt ggatgacggc gagacgctct
    14641 accacttcgt gctgccgaag gcctgcggca acctcgcgtt gaagtcgagt gagcgcagcc
    14701 cgaaggcgat cgccgaggag gcccgcaagg ccgaggaggc gcgcaaggcg caggaagagg
    14761 ccgcccgcaa ggccgaggaa gcgcgcaagg ccgaggaagc gcgcaaggcc gaagaggccc
    14821 gcaaggccga agaggcccgc cgggccgaag aggcgcgcaa ggccgaagag gcgcgcaagg
    14881 ccgaggaggc gcgcctggct gccgaagccg aggccgcccg cctcgaggcc gaccgccgcg
    14941 accgcatcga cttcttcgtg gacggtgcgt tcggcaagga gcggcggctc cgcgaggtcg
    15001 cactcgcggc gccggtcgcc ggggccctcg tggcagagcg cggcgtctgc gcgccgctgc
    15061 tcgggatcaa ggccggtccc gaccttcgca tcgcaccggg ctggcggatc gcgccggctc
    15121 tcggcgtggc gatcaacttc gacgagaccg acaacaccag cctgttcgct gaggccgaga
    15181 tcaaccggtg gttcgggcgc aagggcttca tcggcagcgg catcggcgtg tgggacttca
    15241 cgcacagcga caccgtcgct cccgtgtggc tgctgcaggg cggcggccag ttgtggaagg
    15301 gcaccggcgc gaacgagagc gaactgcacc tggtcgcgca gggccgcctg ttcctcgaca
    15361 agatggacga catcgagaac aactaccagt tctggggcgg cctccggtac atcttccggt
    15421 agcacgccgc cgatcgccag gttcgacgag ggcgctgggt gcgttcaccc gcgccctcgt
    15481 gctttgtacg gatcgcaacg gcgtggccct cgagatcgct ggcagaagcg cgagccgaca
    15541 gcctgagcgt gccgccagcc tgctggtacc cgcattgcag tcccttccct cgagcaccgg
    15601 gaccatcaag cgggagggat acatgtttcg aggcttgacc acactcgtcg tcgcggggac
    15661 gctgggcgtc cttgccgcgg gcgacgtgcg cgcgcagtcg gaaccgcaga gcacgacagg
    15721 gagcggcgca cagtcgagcg cctcgcagga accgcaggga cagcagagcc agcaggccgc
    15781 tgccgccagg cagcggttga tcgaggcccg cgagtcgctc gccgaggtgg cgaagatgcc
    15841 cgaggcagcc gagctgcagg ggcagggacg taccgaggtt tcgcaggcca tctccaactt
    15901 caacgctctt ctcacggccg agtcggactg gtacgaccag tacaagctgg tgatgcagaa
    15961 cctcttcaat gtgctcggcc ccccggaggc cggggagagt gccgcgggca gctcggctgg
    16021 cacgagcggc agctcgacag ccggttcgag cgcaacgggg accgccggcg aggaggttcc
    16081 tccggccctg cgcagcaagc tcgtcgagtt ccgccagaag ctcatggcgt tcggtcgtga
    16141 agcgggcgct ccagacgtga ccggcggcgt cagcagcacg gtcggcgcgg gctcctcgga
    16201 gccgtccagc acttcgggca cctccggcac cgccggtagc ccgccctccg cgcagtcggg
    16261 cacgccggcg gccgagggcg agtcgctgga gcagcacatc gacgccatcg acgacatcat
    16321 ccagcaggct ctggccggca cgcccggcag cggaagcagc acggccggta cgtcggggac
    16381 cactgagagc actgccggcg ccgcgggcgc gaccggcacg acgcccggtg tcgtgacgat
    16441 cgatcgcgag aagctcgatc gcatccagtc gcatttgcag cggatgcgcg agatcgcccg
    16501 gtcgaagggg atcaagtaag cggcaagccg cgcgaccgtc gtcgtagggg cgcaacggtt
    16561 gcgcccctac cggtatactg ccggcatggc gtcgtaccag tacatcttca cgatgaaggg
    16621 cctcacgaag gtccaccccc ccgatcgcaa ggtcctcgaa gacatctggc tcgcgttcct
    16681 ccccggcgcc aagatcggcg tactcggcgc caacggcgcg ggcaagagca cgctgctgcg
    16741 gatcatggcc ggcctcgacc gtgacttcgc gggcgaggcg ttcgccgccg acggcgtgtc
    16801 ggtgggcttc ctgccccagg agcccgagct cgacccgtcg aagaccgtac tcggcaacat
    16861 cgaggagggg gtcgcggaaa ccagggcctt gctcgccgag ttcgatcgcc tgagcgagcg
    16921 cctcgccgga gacctctcgc ccgacgagat ggacgcaacg ctcgacgcgc aggggcgcct
    16981 gcaggaccgc atcgaggcgg ccggcgcctg ggatctcgac tcgcgccttc agttggcgat
    17041 ggaggcgctc cgggtcccgc cgcccgacgc cgacgtgcag agcctctcgg gcggcgagcg
    17101 ccggcgcgtg gctctctgcc gtctgctgct gcgctcgccc gatctgctgc tgctcgacga
    17161 acccaccaat cacctcgacg ccgaatcggt cgcctggctc gagcgctttc ttcacgacta
    17221 tcccggcacg gtcgtggccg tcacgcacga ccgctacttc ctcgacaacg tggccggctg
    17281 gatcctcgag ctcgatcgcg gccgcggcat cccctggcag gggaactact cgtcctggct
    17341 cgagcagaag gagcagcggc tcgagcgcga ggagaagcag gaggcgcgcc ggcagcgcat
    17401 ccttgcccgc gagctggagt ggatccggat gtcgccgcgg gcgcggcagg cgaagggcaa
    17461 ggcccgcctc aacgcctacg aggcgctgct cgcgcaggac gccgcacccc gaatcgagcg
    17521 gggcgaaatc tacatcaacc cggggccacg cctgggcgac gtcgtggtcg aagcctccgg
    17581 gctgcgcaag ggctacggcg accggctgct catcgacaat ctcagcttca cgctgccccg
    17641 gggaggcatc gtcggcgtga tcggcccgaa cggtgcgggc aagacgacgc tcttccgcct
    17701 gatcacgggg caggagccgc ccgacgccgg cacgcttcgg atcggcgaga cggtgcggat
    17761 cggctacgtc gatcagagcc gcgacgcgat gcaggccgac cggaccgtgt gggaggagat
    17821 ctcgggcggc ctcgacgagg tgccgatcgg gaagcgggtg gtctcgtcga gggcgtacgc
    17881 gtcgtggttc aacttcaagg ggcgcggcca gcagcggccg gtcggatccc tctccggagg
    17941 cgagcgtaac cgggtgcacc tggccaagct cctgcgcggc ggctgcaacc tcctgctgct
    18001 ggacgagccc accaacgacc tcgacgtgga cacgctccgc tcactggagg atgcgctggt
    18061 ggagttcgcc ggttgtgccg tcgtcgtcag ccacgaccgt tggttcctcg atcggattgc
    18121 cacgcacatc ctcgccttcg agggcgacag ccaggtcgtg tggttccagg gcaactacca
    18181 ggactacgag gccgaccggc gtcgccggct cggcgccgaa gcggagcggc cgcaccgcct
    18241 caagttcagg aagctcgtta ggagctagga gctacgggcc aggagcgagg ggccagggct
    18301 gggcgcagcg agcgtgcggc acgcttcttg gtctcccctt ttcaccatgc cgaaacggat
    18361 ctcgcccgcg acacacgcgg ccatcgacat cctgctcgtg ggcgctctgc tgctcggggg
    18421 aagcctcctc tggcactgct atcgccggct caggggtccc cgcacccggc ggtggcggct
    18481 gcgcggttgg gagccgctgg gcattggcgc gtagcacgac cgcggcgctt cagaaggccg
    18541 ttccggtatc gaagaccacg cgaaagccct cccggcccca ggcccaagtc catccgcccg
    18601 agcgtcgagt cgccccacag cgcgcgcagg ccgatcccgc cgcccgcctt caaccggccc
    18661 agtcgagccg cgggccgtca tgaggccggc tcggcgtccg gcacgtgcag gcggtctggg
    18721 accgggtctg cctccgtgaa ctcgaccgac gccgagttga tgcagtagcg cagcccggtg
    18781 ggccgcggcc cgtccggaaa gacgtggccg aggtgggcgt cgcagcgcgc acagcgcagc
    18841 tccgtgcgca ccatcccgtg gctgcggtcc acgagctcga cgacgtgctc ggggtcgaag
    18901 ggctcgtaga agcttggcca gccgcttccg gagtcgaact tcgagttcga ccggaacagc
    18961 ggcaggccgc agaggcggca cacgtacgag ccgcgccgct tgttgtcgag gagggtgccg
    19021 cagaagggtg gctcggtgcc ctggtggagc acgatctgcc gctcctctgg cgacaggcgc
    19081 gcggcaagcc ggtgtatcgt ctcgccgtcg agcggggtga gatcgtagcc ggaggccgac
    19141 ttcctggtgg gcatcgcgct gctcccttga ctggcccgtg ccagcaacgt tcctgccgga
    19201 tggtccatct tgtcacaacc ggcgcgtcga agcgtgctcg tccgccccag ggccggctcg
    19261 acgatctttc gttttctgca gccgcgcggc tagaatccga cgatgattct gccgtcactt
    19321 gccgcgctgt tcttcctcct ccagtcgtcc gccgccgctc cctcgcagcc cgacctgtca
    19381 cccggaacgc gatacgaccc gaaggttcct acgctgcagc aggcggttgg gcacgacgtg
    19441 ggcgaacgta tctcgtcgcc cgaagagatt gccgcctacc tgaaggcgct ccatggcgcc
    19501 gatcccgccc gctcgcgtct cgtcgagtac gcgcgcagct gggaggggcg tccgctgcac
    19561 gtgctgatcg tcggttcggc ggagagactg gcgaagctcg accaggtgaa ggccgacctc
    19621 cgccggttgg ccgacccgcg gcagttgccg gccgccgacg gcgaccgcct ggtccgggaa
    19681 ctgccggtcg tgacctggct gctgcactcg gtgcacggcg acgagaccac ctcgtcggat
    19741 gcggcgctcg ccgaggccta tcacctgctc gccgcgcgcg gcagcgaggc ggtggacacc
    19801 attctccgcg agtcgatcgt gctcatcgat ccgctgcaga atcccgacgg gcgcgcccgc
    19861 ttcctctcgc acaacctgca ggcggcggcg gcccgtcccg accccgaccc gttggcggcg
    19921 gagcacgacg agccctggcc cggcgggcgc tcgaaccact acctgttcga catgaatcgc
    19981 gactggctcg cgctctcgca gcccgagacg cgcgggcggg tggcccttgc cctcgagtgg
    20041 tttccgcacg tggtggccga cctgcacgag atggggggcg actcgaccta ctacttcgcg
    20101 cccccggccg agccggcgaa cccgtacatc accgcgaagc agttcgagtg gctcaacgtc
    20161 ctcggccgcg agaacgcacg gcgcttcgac gagcggggct tcgcgtactt cgtacgcgag
    20221 gtgttcgact cgttctaccc cggctacggc gagtcgtggc ccatcttcca cggcgccatc
    20281 ggcatgacct tcgagcaggc gtcgcccagc gggttgctgt tccgccgcga cgacgacacg
    20341 gtcctcagct accggcaggg catcgtccag cacttcacgg cggccatcac cagcgccgag
    20401 acggccgccc gcaaccggga gcagatcctg cgcgacttcc tggagtaccg gcgcagcgcg
    20461 gtacaggaag gcgagcgcgg cccggtgcgc gagtacctga tcccgccggg cgccgaccgc
    20521 gcgcgccacg acaggctggc cgagctgctc acgctgcacg gcctcgaggt gcgccgggcc
    20581 ggtgagccgg tgaaggcggg ctcgcgcacg cttccggccg ggaccctcgt gctcccgctg
    20641 gcgcagccgg cgtcgcggct gctgcgcaac gtgatcgaac ccacgatcca gatggacgag
    20701 aagttcttga aggagcagga gcgccgccgg cagaagcggc tcggggatca gatctacgac
    20761 atgacggcct ggagcctgcc gctgctgttc gacctcgaga tcgtcaccct tccacagccc
    20821 tccggcgcga aggcgtcggt ggtcaccccg cgccctgcga cacccggcgg cccgcttccg
    20881 gcggcgaagg tcgggtacct gcttccgtgg ggcagcggca cggccgcggt ggtggcggag
    20941 gcgctgcagg cgggcatccg cgtcagggcc gcaggagacg ccttcacgca agccgggcgg
    21001 aagtacccga ttggcaccgc gattgtccgg atctcagaga acgggccgga cctcgcctcc
    21061 cggctcgggc cgatcgtcgc ccggcacatg gccgaggccg tccctctcga gaccagctgg
    21121 gtcgaagagg ggatctcgct tggcagcgac cgcgtcgtgg cgctcaaggc gccgcgtgtt
    21181 gcgatggcgt gggacgtgcc gacctcgagc gcctcggccg gctgggcgcg gtacgtgctc
    21241 gagcgccgct tcggacagcc ggcgactgcc atccgcaccg ccgccctccg aaacgccgac
    21301 ctcacgcgct acgacgtgat cgtcctgccc gccggcgact acacgcaggc gctcggggag
    21361 gacggcgtcc ggcggctgaa ggagtgggcg cggatgggcg ggacgatcgt gacgctgggg
    21421 gaggcgtcgc ggtgggccac gcgcgagaag gtcgcgctgc tcgagacgac gaccgagctg
    21481 cgcggcggca aacccgacgt cgagccggcc ggggacgaga agccgaagaa ggttgcggcc
    21541 ggggagccgg ccaagccgtt cgacctccag caggcgatcc aacccgaccg cgatccgccc
    21601 gacgccgtgc ccggggccat cctccgcgtc tcgctcgaca ccgagcactg gctcagcgcc
    21661 ggcctcgacg acgagatcca ggtcgtggtg gaggggcggc gcatcttcac gccgatcaag
    21721 ctcgacaagg gacgcaacgt gggcctctac gcggcgaggg acaggctgct cgccgccggg
    21781 ttcgcctggg acaacccgat ggagcagctg ccgcagaagg cgttcctgct gcaccagccg
    21841 ctcggccgcg gccacgtgat cgccttcgcg gaggatccga actaccgcgc cttcgccggg
    21901 tgcaccgagc tgctgttcat gaacgcggtg ctactcggcc cggcgcattg aggtcttctg
    21961 ctacgccccg tcccgcgcgt gttcgtgccc gtgcaggtgc gggtgcgagt gctggcggcc
    22021 ggggtgggtg tgccggtggg cgtgaaccag gttggcgcgc tggaggaggg gcagatccgc
    22081 gaggatgacc gcgggcgccg cgtcggcgac gaggcgcccg tcgttcagaa cgcagcagcg
    22141 atcggcgatg tcctcgaggg cgccgagatc gtgcgtcgcc gtgaccaccg tcttcgcgcc
    22201 gcccgcccag ccgaccagca ggtcgatgac ctcgctctcg cttcgcgggt cgaggcccgc
    22261 ggccggctcg tcgagcagca ggacgtcggg gtcgagaacg aggacgctcg ccagcgcgac
    22321 ccgcttcttc tcgccggtgg agaggcgctg aggcacgcgg tcgcgcaggt gtgccacccg
    22381 cagcttctcc atcgtctcgc ccacgcgcgc atgcagttcg tcgcgccccc acccgagctg
    22441 gagcggcccg aacgccacct cgtcgaagac ggtcgggttg aacagctgcg cgtcggcgtc
    22501 ctggaacacg aggccgacct ggcgcctgaa cgagtaggcg aagccgtccg gggcgaacgc
    22561 cgcctcggtg agcggcgtgc cgcggtagcg cacgaggccg gcgtcgggga acagcaggcc
    22621 gtccagcagt cgcagcaggg tcgacttccc ggagccgttc gccccgacca gcgcgacacg
    22681 gcttcccgct tcgattgaca gcgagagccc ccgcagggcg gtgacgtcct ggtaccggca
    22741 cgtgacgtcg ctcaactcgt agaccattac tgccgtccca tccagacgca caaggtcgcc
    22801 aacacggcga aggcgccaag cgccgcccag tcgcgcgggc gcatgcggaa ctggtcggcg
    22861 acgtggacct cgccgcggaa gccccgcgac tgcatcgcca ggaagacgtc ctggctcagt
    22921 cgcatcgagc gctcgagcag cactccggcc gccgcggcgg tcgtccgcct tcgcaccggt
    22981 cccggaagcg gcccgaccgt gcggctgcgc cgcgcgtcga acatcgcgtg cgccgcctgc
    23041 aggagaacga ggatgtacct ccaggtcatg cccagcacga ccaccgcgac cgccggtaca
    23101 cgaaaggttc tgagcgcttt caggacgtgg gtccaaggcg tggtgaacac cagcaggaag
    23161 gccagcgtcg ccgccccgag gacgcgcaag aggaggtacg ccgcgctggt cgccccctgc
    23221 acggtaacga tcagaccggc gccgggcagg gtgaaccact cctggccggg cgtcgtgaac
    23281 agggcgggga gggcgatgat cccggtgaac gcgagggtgc caagccagag ccgcgtgacc
    23341 agcaggcgaa ggggcagccg cgaggccgaa gcggccacga ccgccaggag gaggagaaac
    23401 gccagcgtgg cgaggttgcg cgcgagcgcc accgcggcga cgagcgccag cacgccggcg
    23461 agcttcacgc ggggatcgag ctgctgcagg agaccccggg tgcctgcccg gcgctcggct
    23521 tccctcgcgc ggtccatgac ctcgagcagc ccgtcaaggg tgcgttcgac gtaaccgcgt
    23581 cgcctcgtca ctcgtcctca cccggaagct tgcccctcgc gccgccccgg ccgccccagg
    23641 cgttcgagca ggaggaacgc gagaatcacc agcccgacgc cgagcaccgc cgacaggacg
    23701 tagccgaagc gggcgctgcg cacgaacggg ggcgcgtaat cgggccacgg ggccgtccag
    23761 acggccgaca ggcgatcgag gcccgaaggc gccgcggccg ggggcgccac gccgagcgac
    23821 gcgtcggcaa tctcctggcg cgccgcggga tcgttgaagt cttcggccgc ccactcgccc
    23881 caggccgttc cggcggccag gatgccgagc ggcgccagca ccatcaggat gccgagccca
    23941 gcccacaacg ggcgaaggct tccgccgcgt gccccattcc cgggggccgc cggagccgtc
    24001 attcccgcgc cgcgcgcgag cagcgacggg tccgatcgct ggatgaacgc gacgacgccc
    24061 gccgagacga ccagctcggc gagccccgcg atcgtcaggt ggccgagcat catggcgggg
    24121 accgcgattc gcagcgggta cggggcgtag agcggggctc ccgacgcgtc cgcgaagaac
    24181 atcggctgaa ggccgaactc gatggcggtc agcagtgcgg ccaggttcac ggccacgtac
    24241 cccgccaggc ccgcggccat cgcccggcga ggcgaggaaa ggggcgcgtt tcccgcaacg
    24301 gcgcggtaaa cggcccaggc ggccagcgag cccgccaccg ccatgttgaa gcagttcgcc
    24361 ccgagcgtcg tgatgccgcc gtcgccgaag aagaacgcct ggatcgccag cgccaccgag
    24421 atcgccagca ccgacgccca cgggccgagg acgaccgccg ccagcgccac accggtcgcg
    24481 tgccccgtcg tcccgccggg cagcgggatg ttgaacatca tcacgacgaa cgagaacgcc
    24541 gcgaagagcg agagaagcgg cacgagccgc gtgtggagcg cggcgcggac gcggcgcagg
    24601 gcgacgtacc agaacggcgc cgcgccggcg tagagcgcgg cgctggtgag cgggctgagg
    24661 tagccgtcgg ggatgtgcat gatcgtgtat cgcaggccca gcgtagcaca ccggcaacgc
    24721 gggctcgtgc gccggcggag cagggcttcg acggcggtgg cgcaggccct tggcctggca
    24781 ctctcagcct cgggtatgat ggcga
//
LOCUS       DAOPQX010000011        24198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2613_length_24198_cov_4.821646, whole genome shotgun sequence.
ACCESSION   DAOPQX010000011 DAOPQX010000000
VERSION     DAOPQX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 24198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24198
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2613_length_24198_cov_4.821646"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKK95_01295"
     CDS             <1..493
                     /locus_tag="PKK95_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC16867.1"
                     /translation="ILPAVLYFRDRGIVAVTSENFDGEWIARLMRRFGYAAARGSTSR
                     GGSRALIQLRRDMAAGRPAAFTVDGPRGPARRAQAGAVWLAKATGNPVVPFHIEAARG
                     WELPSWDRGLIPRPWSRVAIVLGAPLEVPGDADPGGIEAARAELERRLAALEDEALAL
                     LAG"
     gene            661..3237
                     /gene="leuS"
                     /locus_tag="PKK95_01300"
     CDS             661..3237
                     /gene="leuS"
                     /locus_tag="PKK95_01300"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOC16868.1"
                     /translation="MREYRPLELDRKWQQRWAETRAFEVSEDAGKPKFYCLEMFAYPS
                     GHAHIGHVRNYTIGDVMARLKRMRGFNVLHPFGWDAFGLPAENAAIKGGLHPEASTRA
                     NIAHMKGQLQRMGISYAWDREVATCDPEYYRWNQWLFLKMLERGLAFRRLAPVNWCPS
                     CQTVLANEQVVGGGCWRCGTEVATRNLEQWFLRITEYAQELLDATGGLTGWPEKVLTM
                     QREWIGRSEGARVRFPLLAADPQRTDGETGIDIEVFTTRIDTIYGATFVLLAPEHELI
                     ERFAAESPDPGAFRAQAQRFRAQDRMARLSGEIEKEGFFTGRYARNPFTGAEVPLWVA
                     NFVLGEYGTGAVMAVPAHDQRDFEFARKYGLPVTVVIQPEADGTVGSLDGGTMGEAYV
                     EAGRVVNSGEFTGLWSVEAIAAMTAAAEARGIGSRTVQYRLKDWGISRQRYWGTPIPV
                     LYCGACGIVPVPYEQLPVELPSVAEFSGRGSSPLAQVTEWVETTCPKCGGTARRETDT
                     MDTFFDSSWYFYRFCDPHNDRLPFDPEKVAYWCPVDFYSGGVEHAILHLIYSRFFCRV
                     FRDLGMVQHDEPFTRLLTQGMVLATDGSAMSKSKGNTVDPDDLIERYGADAVRLYVMF
                     MAPPEKEVAWSDAGVEGSARFLARVWRLVDHWSDAVAGAPADFSRLDLTEAEQGLRRA
                     AHDTIRRVTHDLDPRVHLNTAVSALMELVNALYAFSEEQTGLGVPGRRAAGTEVRAAE
                     RPEALAVLREALDALVLMLAPFAPHTAEELWEMLGHRDGLACAAWPSYDPVVARSSEI
                     VVPVQVNGKLRGRLTVPADIGEDALREAALAEASVRVHTAGKTVQKVIVARGKLVNVV
                     AR"
     gene            3305..3850
                     /gene="lptE"
                     /locus_tag="PKK95_01305"
     CDS             3305..3850
                     /gene="lptE"
                     /locus_tag="PKK95_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOC16869.1"
                     /translation="MNRFARLRTAAVVLAACVSASCGYALAGRGSFLPDSIQVIGIPN
                     FGNRTPYLEVEQVLTQRVRSEFIGRGKYKVLPQDTGVDAVLVAEITNISIAPASFNED
                     QQASRYTISVVVSARLVEAGTDKVLWQNPSQVFREEYALSSGGESVLDPAAFFGQSSN
                     AVDRLAADFAQALVTAILEAF"
     gene            3929..4915
                     /locus_tag="PKK95_01310"
     CDS             3929..4915
                     /locus_tag="PKK95_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16870.1"
                     /translation="MARGTLTSAAFRRQIASGELGPLYLVTGPDDVEMSRLAAALTES
                     VPEDFRAFNVHRFHGTDKVTLAQVLDAASAYPLLAPRRVVLLLQAGRVLGKKGQADDE
                     PEEAGPDDETVGEAVERPGAPRKTGGPAPLQVLKQYAAAPHDHATVAIFAPGLQRAFE
                     DIGGRAVMVFCEPDQDPINDLAAEHGVRFHPAAAQLLRQRAGDDLERLKADAERVLLY
                     AAGSQVITREQVEAVSGRQAAAGGRALWGAVAGGRTAEALRELNLEMAEGAVPFMVLG
                     LVRSAVERTVSGPALAAAYDALLRTDLALKSSGGDPQVLLERLVVEMCSLTQ"
     gene            complement(5086..5352)
                     /gene="rpsT"
                     /locus_tag="PKK95_01315"
     CDS             complement(5086..5352)
                     /gene="rpsT"
                     /locus_tag="PKK95_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOC16871.1"
                     /translation="MASHASALKAHRQSLKRREQNRRFRSQLRTAIKSIRASVAAKDA
                     DTARGALSKTVALIDRMAGKGIIHRNAAARYKSRLQVGINKLSA"
     gene            5571..6272
                     /locus_tag="PKK95_01320"
     CDS             5571..6272
                     /locus_tag="PKK95_01320"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOC16872.1"
                     /translation="MSISIVGGLEDIMSAMEVRCPAVAAHCRRVSLYSVRLATQYGLD
                     TETVEAIRVGALLHDLGKLEVPPRLLDKPGRLTEREWDRLRRHPEAGLALVDRLGFDA
                     PVGEIVLYHHERFDGSGYPDSLAAERIPWPVRIVSVMDAFDALTSPRPYRAALSLDAA
                     RSLLAREAGTRYCPWVVAGLLSLPRPLLHGVASEPPHQCRPDGCPSPAALLKATQAWN
                     RHAALADHGCLSATA"
     gene            6369..8228
                     /locus_tag="PKK95_01325"
     CDS             6369..8228
                     /locus_tag="PKK95_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC16873.1"
                     /translation="MTSTRRVRWATLLFLLAGGVSIGAQEPPAAARPAPATRAVQQER
                     IDAASVRAAEGLDTFLPRRTDSILRALASRFEPSRAMDLVSFMDRFWRVPGNSGYNAS
                     LERIRSGLTAAGFAEAKGPGASSPSLWFEEYPNGGNGWEPVSFAMTILEGEAGPAERV
                     FDPVIDYIALCMNSFPTPPGGLTARLVYVGDGNKPEDYASVDVKGAVVLADGGMRRVW
                     QEAVRARGAAGVVSAERPPDYARPDETPEIFQWGGIPYDEKARAFGFKASPKVAGRLK
                     ERLKKGPVTVRVEIETRFDPSPGRMLVAEIPGATRPAERIVMVAHVQEPGANDNATGC
                     ATLLELARAAAAATASKALPRPGRTLTFVWGDEMRASREWLRAEPGRAASARAMISLD
                     MTGEDAAKTGGTFIIEKEPDPSAVWPRPSDPHTEWWGGGAYQRALKGSYLNDLLLAVC
                     LRRARETGWVVQTNPYEGGSDHSIFLNAGVPALLVSHFTDRYYHTNLDRPDKVSATEM
                     AHVGISVGTAVMLIASAGDEEARAVADLVADAARRRLELEARQSAELIAGAADRTAAE
                     AGERVLHDGWRAWYASALESVLTLPQAPAGRALRARIRTAIDAMGAAAAGAAR"
     gene            8225..8965
                     /locus_tag="PKK95_01330"
     CDS             8225..8965
                     /locus_tag="PKK95_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC16874.1"
                     /translation="MIEVEGLTKVYGRLTAVDRLSFGVRAGEVVGLIGPNGAGKTSTL
                     RCLAGIQAPSAGTARIAGFDIVSQPIEAKRRLAFMPDEPQLFEYLTVAEHLRLVGRLY
                     GVPRVDERSGTLLEELDLAGKQHALPGELSRGMKQKLMIACGLLHDPQALLFDEPLTG
                     LDPIGIRRMKETIVARARNGAAVLVSSHLLHLVEEICSRIVIINKGTAIADGTLEELA
                     ARADLTADSDLEDVFLKATGHDRGGRAT"
     gene            8962..10677
                     /locus_tag="PKK95_01335"
     CDS             8962..10677
                     /locus_tag="PKK95_01335"
                     /inference="COORDINATES: protein motif:HMM:NF028272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HOC16875.1"
                     /translation="MTWAFLYLTARSLRNRVAARLRRLRQPRYLAGLVAGLAYLYLFI
                     LRHQVRAVRRGDFTVDPQFASIAPAVLVAGGLLLWVVSLAAWLWPSTEPPLRFSRPEV
                     QFFFTAPVSRRQLVHYKLLRAQLGIVFGLAIVSLFSGAAVSGRIWFLLGGWLLFATVR
                     LHLVGVAFTRAFTRASIARRRGRPLRSAWPALAAIGALSAAILGTWLANLPALVSLPF
                     GAAMRRVFDVFSSGPAAAALWPFSALIAPVLATNTRQFLASAWPAALLLALNYWWVLR
                     SETTLEEAAGAAEREQAGGRRQAPKPVTRRAPFALAPSGRAEVAILWKNLILGGRYLT
                     PRVVLRLVVPLLVLAAAAAVGEFALGVAPAALIVAGALTLLGPYMVRYDLRHDLPRLT
                     VLKTWPVRGATLVRGEVLAPALVLTSGVWIMLAIALVLAEGLSYGGLSLAARVWLGLA
                     AAFAAPSFLLAQLVIQNAAVIVFPGWIPTGSNRPRGVEAMGQQMLMFAGTLLLMILGV
                     LPAAAVAGAAGFVLFQVIGWAAVAAAALVFSAALAAESLLAIELLGRLIERTDPVDVE
                     AEEPV"
     gene            10830..11555
                     /locus_tag="PKK95_01340"
     CDS             10830..11555
                     /locus_tag="PKK95_01340"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOC16876.1"
                     /translation="MPRGLPIVLFGLAIVSGVAAPASAQIYSYRDASGSLVLSDAAPS
                     DPSLSVRTFEAVNTSERVRVTRPVSKAYREQFDELIVHYAEAQGIRPDLVRAVVQVES
                     GYNPRALSPKGAMGLMQLMPDTAAALGVKRPFDPEENVRGGVAYLRHLLDRFNGNEEL
                     ALAAYNAGPAAVGRYGNRIPPYRETRDYVRKVRTRTTVSAAAAKPKLVIYKWVELVEG
                     KPVTRYSDSPPATGSYERVPGGR"
     gene            11633..12004
                     /locus_tag="PKK95_01345"
     CDS             11633..12004
                     /locus_tag="PKK95_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOC16877.1"
                     /translation="MQDHRQLEIWKRSMDYVVEVYQWCLQLPKDERYNLTDQLKRACT
                     SVPLNIAEGAACETNGDFARFLGYSYRSLKESVTCLELCERLYPSLSNAPTCLINEAE
                     QLAKMTRTLIQRLKPPFIQET"
     gene            complement(12136..14838)
                     /gene="alaS"
                     /locus_tag="PKK95_01350"
     CDS             complement(12136..14838)
                     /gene="alaS"
                     /locus_tag="PKK95_01350"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOC16878.1"
                     /translation="MTSSEIRASYLDFFARNGHRVVASSPLVPANDPTLLFTNAGMNQ
                     FKDVFLGRERREYTRAASSQKCMRVSGKHNDLENVGPSPRHHTFFEMLGNFSFGDYFK
                     TDAIAFAWTLLTEVWQLPPERLHATIFRHWDKVPQVPRDEDAHAIWRKYLPAERISEL
                     GEDNFWAMGDTGPCGRCSEVHYFRGDHLPCPEPVCLGVACSCDRYVEIWNNVFMEFDR
                     QPDGTLNPLPAPSIDTGMGLERITAVLQGVLSNYDTDLFTPLLGAIGALAGARYGGSM
                     EPRDVSMRVIADHARAATFLIADGVVPSNEFRGYTLRRIMRRAMTHGKHLGMTEPFLH
                     RLVAVLAGEMGDAYPELREQREYVERMIHAEEERFESVLRIGLPKLEDSLERAAAAGG
                     VLAGDEAFRLYDTFGVPRDFIEDMAGQRGVAVDRAGFERAMEGQKEKARARHAFGLAT
                     AGSAALEAATETLRGRPDQFEGYRTTRLDNLAVIALLDEDGRPVPELREGQGGYLALD
                     RTPFYAEAGGQVSDTGAITSTSGARARVEGLVRVGADRPRLHEVVVESGTISDRDTLV
                     AEVDEELRNATRRNHTATHLLHAALRQVLGSHVKQAGSLVAPDRLRFDFVNPDPVTSE
                     QLAEIERLVNAEILRNTPVETEVRATEEAIAAGAMALFGEKYGDRVRVVSVPGFSQEL
                     CGGTHVAATGDIGCFVIVHESGVAAGQRRIEALTGHGALQFYREQRRTLAGVLEALKV
                     PADQAAGAIERLQADLKRLSRENSQLKVQLATASLAGAPATAATPAGQADAGGITLVA
                     RRVQGLDKAGLGQMADSLKNQVKSGVVVLASENDGRVAIVVSVTKDLVPKIHAGQIVK
                     RLAPMVGGGGGGRPDFAEAGGKDPSGIERMLEEARGVVEEMRRG"
     gene            complement(15017..15493)
                     /locus_tag="PKK95_01355"
     CDS             complement(15017..15493)
                     /locus_tag="PKK95_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOC16879.1"
                     /translation="MSNPAYSAALAMLARRELSERQLRERLERREHPPTAIEEAVTRL
                     RASGLLDDRRVARAAARVEAQVRSRGRLRVLQRLHALGIAPEVAAEAADEVFGAIDED
                     TLLEAALARRLRGPAARIRDAAHFRRLHQQLVRQGFAPSAVTRALKRHWRGELEQE"
     gene            complement(15504..16664)
                     /locus_tag="PKK95_01360"
     CDS             complement(15504..16664)
                     /locus_tag="PKK95_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOC16880.1"
                     /translation="MHVNDLLKIAVESGASDLHLKVGSYPMMRVAGMLTPASEEKRLT
                     HEDTVTMAASVMSAAQRQKFKEAQEVDLAYSVQGLGRFRCNVFQQRGTIGIVLRVIPF
                     GIRTIDELGLPAVLARIADEDRGLVLCTGTTGSGKSTTLAAMVDHINNTRCTHVVTIE
                     DPIEFLHRDKRSIVNQREVAVDTHSFAQALRSALRQDPDVILVGEMRDFETIETALVA
                     AETGHLVFSTLHTLDATETINRIIAVFPPHQQKQVRLQLASVLKAVISQRLIPRADGL
                     GRCAAVEVMVTTPFIRDCVVDKEKTHLIPGAIAAGTSQYGMQTFDQSIFSLYEQGIVS
                     YEEALHWATNVDEFKLKVQGIAMTSDAARDEMARAGFGGGPAAAPEVQRFRQ"
     gene            complement(16710..17684)
                     /locus_tag="PKK95_01365"
     CDS             complement(16710..17684)
                     /locus_tag="PKK95_01365"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOC16881.1"
                     /translation="MAFPETGTIWMNGSLVAWKDATIHIASHVIHYGSGVFEGARCYE
                     TPKGPACFRLDAHLRRLYNSAKIYRMEYRAGLEEFGEAVLDTIRANNYRACYIRPLIY
                     RGYGAPGVNPLGSPVEAAILVWEWGKYLGPEALEQGVDVRVSSWRRSAPDTFPTLAKS
                     SANYASGQLIKMEAVVEGYTEGIALDTFGYVSEGSGENLFVVRDGILYTPPLQSSVLP
                     GITRDSILTLARDLGYPIREEVIPREALYIADELFFVGTAAEVTPIRSVDKIVIGEGR
                     CGPVTKRLQQAFFDTINGVLPDRHGWLTYVYPGKGSADKTTQENAVTR"
     gene            complement(17758..18744)
                     /locus_tag="PKK95_01370"
     CDS             complement(17758..18744)
                     /locus_tag="PKK95_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC16882.1"
                     /translation="MFFRGQIIGKYQILSALGSGGFGAVYLAEDTWIQKKVALKVPHR
                     QNIDFGELIKEPRLLASLSHPNIVSILTAEKQENVFFIVMEYVQGETLENIIGRTGAL
                     ELTRALDFTCQICNALDHAHTQGVLHRDLRPANVLVSDAGMAKVADFGTARFLEIASQ
                     GTTVIGSPPYMAPEQFQGKAVFASDIYSLGVTMYQMITGSLPYDTPSPAALQKLMRGE
                     LFQPPRARNSRIPRELNEIVMRAMAPAVNDRYQRAGELLDDLLAARSKWVSSRAGRPP
                     VTPVRPGPASSQLQEIQGRLRAREAPSPRFCWQCRKPLHPRADRCPFCGEAQ"
     gene            complement(18840..19577)
                     /locus_tag="PKK95_01375"
     CDS             complement(18840..19577)
                     /locus_tag="PKK95_01375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOC16883.1"
                     /translation="MHVRFHAPGLEPGDRSAVLSAEESGHAVRVLRVRRGAEVRLFNG
                     RGYECEAVVENPAPAGLTLRVVGSVCACPEPRVALTLAQAVLKGDGMDEVVRDAVMLG
                     VRAIVPVVSTRVEADRARLLKARRHERWQRIAVSSAKQCGRAVVPEVREPVTFEEAVA
                     GLGDPIRLMLAEPAVDGPAALDLPSLPGPVSAAILVGPEGGWTAAEVSLAASSGWSRL
                     TLGTRTLRADAAATVAIPVLQYLWGDL"
     gene            complement(19648..20496)
                     /locus_tag="PKK95_01380"
     CDS             complement(19648..20496)
                     /locus_tag="PKK95_01380"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOC16884.1"
                     /translation="MPKAWPALDVRFAAVTPEARAETADLLAAALDDLNPTAIEEQDA
                     GWRVFFASDAGRDAAGRALAGLGPVVEPRPIEVPDEDWARRSQEKLGPVRVGRVLVTP
                     PWLTPPAQDGDLVVTILPSMGFGTGHHQTTRLCLALLQARNPSGLHVLDVGTGSGVLA
                     IAAARLGAASVVALDDDADAIAAAADNLGLNGAAEAVTLRHGDFRGMAGLAADLVLAN
                     LTGALLIGQAEALAGAVAPGGSLIASGLTLDEEEAVTAALGAQLMTPVSRLVEDEWVG
                     LRFDRR"
     gene            complement(20501..21643)
                     /gene="dnaJ"
                     /locus_tag="PKK95_01385"
     CDS             complement(20501..21643)
                     /gene="dnaJ"
                     /locus_tag="PKK95_01385"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOC16885.1"
                     /translation="MSKRDYYEVLGVGREATDQEIKSAYRKLALKFHPDRNPGNKDAE
                     EKFKEAAEAYSVLADADKRAAYDRFGHAGVAGAGAGGFDPSIFQGFGDDIFGGLRDIF
                     GDIFGMGGGRRGGPQRGADLRYDLEIEFEESARGLETTIQLPRQETCETCRGTGAAPG
                     SSPSVCPHCGGRGQIRYQQGFFTVAQTCRQCGGSGRIITRPCSDCRGAGQVARERKLA
                     VRIPAGIASGQRLRLYGEGEGGVAGGPPGDLYVVVHVKDHPVFRREGDDLVCRVPVTF
                     PVVALGGTIEIPTLDGTEPLEISAGTQSGHVFRVRGKGMPSVNGRGRGDLHVVVQVVT
                     PKKLTREQRTLLEQLSKAMPIEKVKAPKGDADEEKSVFDRVKDIFS"
     gene            complement(21640..22173)
                     /gene="grpE"
                     /locus_tag="PKK95_01390"
     CDS             complement(21640..22173)
                     /gene="grpE"
                     /locus_tag="PKK95_01390"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOC16886.1"
                     /translation="MEQRDDTAARPLEEAAPAEERVESGAAAEPDLAALERERDEYRD
                     LLLRKSAEFDNYRKRVERERRELVQYAASEVFEGLLPILDDFERALQAPAGGSAEAYR
                     QGIELIHKQLVDFLAKRGVKPIEALGSSFDPRFHEAITYEERPGSRDGEVVEEIRRGY
                     VHGDRLLRPAVVRVAKA"
     gene            complement(22180..23238)
                     /gene="hrcA"
                     /locus_tag="PKK95_01395"
     CDS             complement(22180..23238)
                     /gene="hrcA"
                     /locus_tag="PKK95_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOC16887.1"
                     /translation="MYTSRLSDRSRRILAALVAEYVETGVPVASGTLVLKGGLGLSSA
                     TVRNVLASLEEQGYVYQPHTSSGRVPTDLGYRCYVDMLLESRRPSRVPGIEAQLLAQT
                     GGAPLMEEALTTASHVLSEASHHVGFALAPAAEEDAFQRIEFVPLGDTRILVVVVMRR
                     GQITNKVVDIDERIAADDLHMAANYLNEEFAGRPLQKVRESVLARLAEERTLYDRLMS
                     RALRLAQSTFDELSAQQALFIEGFAGLVEETAEGSVSLGTLRALLKMIEEKHRLLRIL
                     AEYMEGPGLTVIIGEEHRAPDLRPFSLVTASYTDGDRRGSIGVIGPTRMRYSKAISIV
                     DSAARTITRLLQQGGTGD"
     gene            complement(23315..>24198)
                     /locus_tag="PKK95_01400"
     CDS             complement(23315..>24198)
                     /locus_tag="PKK95_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOC16888.1"
                     /translation="GGWGLGAGGYELGAGQMAVDVFKALVEAAARGEAAALVTVVATS
                     GSTPQRVGAKMIVFRDGRTLGTIGGGCYEHDAAQKAREALATGRPELLHYSLADELAA
                     DTGLICGGQMEVFVEPIRQPARLYIAGAGHVSVELARLASSVDFAVTVVDDRERFANR
                     ERFPAAEVVVDEIPAWFEAQELPPDGYFAVVTRGHRQDLETVRALIGRPWIYLGLIGS
                     RAKVLKVMDALAQEGVPRERLEALHAPIGLDIGAVTPAEIAVSIVAELVAARSGRLTA
                     AHVARCSLRSSLSRVTR"
BASE COUNT         3389 a         8550 c         8594 g         3665 t
ORIGIN      
        1 catcctgccc gccgtcctct acttccgcga ccgcggcatc gtcgcggtga ccagcgagaa
       61 cttcgacggc gagtggattg cccgcctcat gcggcgcttc ggctacgccg cggcccgcgg
      121 atcgaccagc cgcggcggca gccgcgccct catccagctg cgccgcgaca tggccgcggg
      181 acggccggcc gccttcaccg tggacggccc gcgcggcccg gcgcgacggg cgcaggctgg
      241 cgccgtctgg ctggccaagg cgaccggcaa cccggtggtg ccctttcaca tcgaggccgc
      301 ccggggctgg gaactgccca gctgggatcg cggcctcata ccccgcccct ggagccgcgt
      361 ggccatcgtc ctcggtgccc cgctcgaggt gcctggcgac gcggacccgg gtgggatcga
      421 ggcggcacgc gccgagctgg aacggcggct ggccgcgctc gaggacgagg ccctcgcgct
      481 gctggctggc tgatcggcgg cacggcgtcc cccgacgccc cgcgcgacag ggacgccgca
      541 gggctaaagc cccgcgtgac ggacgccgcc gccgggcaag agcctgcgct acggtgctga
      601 cgcctgcctg tcgagctggc tcccgccggg tataatcaca ggattctgga ggacgtgttc
      661 gtgcgcgagt acagaccgct ggagctggat cggaagtggc agcagcgctg ggccgaaacc
      721 cgggccttcg aggtgagcga agacgcgggc aagccgaagt tctactgcct ggagatgttc
      781 gcctacccgt cgggccacgc ccacatcggg cacgtccgca actacacgat cggcgacgtg
      841 atggcccggc tgaagcgaat gcgcggcttc aacgtgctgc accccttcgg ctgggacgcc
      901 ttcggcctgc cggccgagaa cgccgcgatc aagggcgggc tgcaccccga ggcgtccacc
      961 cgcgccaaca ttgcccacat gaagggtcag ctgcagcgga tggggatcag ctacgcgtgg
     1021 gatcgcgagg tggcgacctg cgaccccgag tactaccgct ggaaccagtg gctgttcctc
     1081 aagatgctgg agcgcggcct cgcgttccgg cggctggcgc cggtcaactg gtgcccgagc
     1141 tgccagaccg tcctcgccaa cgagcaggtc gtcggaggcg ggtgctggcg ctgcgggacg
     1201 gaggtcgcca cccggaacct cgagcagtgg ttcctccgca tcaccgagta cgcgcaggag
     1261 ctgctcgacg ccaccggcgg cctgaccggc tggcccgaga aggtcctgac gatgcagcgg
     1321 gagtggatcg gccggtcgga gggtgcgcgc gtgcggttcc cgctgctcgc cgccgatccc
     1381 cagcggaccg atggggagac gggaatcgac atcgaggtgt tcacgacccg catcgacacc
     1441 atctacggcg cgaccttcgt gctgctggcg cccgagcacg agctgatcga gcgtttcgcc
     1501 gccgagtcgc ccgaccccgg cgccttccgg gcgcaggcgc agcgcttccg ggcgcaggac
     1561 cggatggccc ggttgagcgg cgagatcgag aaggagggct tcttcaccgg ccgctacgcc
     1621 cgcaacccgt tcacgggggc ggaggtcccc ctgtgggttg ccaacttcgt gctcggcgag
     1681 tacggcaccg gcgcggtgat ggcggtgccg gcgcacgacc agcgcgactt cgagttcgcg
     1741 aggaagtacg gcctgcccgt gacggtcgtg atccagcccg aggccgacgg caccgtgggc
     1801 agcctggacg gcggcacgat gggggaggcc tacgtcgagg ccgggcgcgt ggtgaactcc
     1861 ggcgagttca cgggcctgtg gtccgtcgag gcgatcgcgg cgatgaccgc cgcggccgag
     1921 gcccgcggga tcggctccag gaccgtccag taccgcctga aggactgggg catttcacgg
     1981 cagcgctact ggggcacgcc aatcccggtc ctttactgcg gcgcgtgcgg catcgtgccg
     2041 gtcccgtacg agcagctccc ggtcgagctc ccgagcgtcg cggagttcag cggccgcggc
     2101 agctctccgc tggcccaggt gaccgagtgg gtcgagacga cgtgcccgaa gtgcggcggc
     2161 acggcgaggc gcgagaccga cacgatggac acgttcttcg actcgtcgtg gtacttctac
     2221 cggttctgcg acccgcacaa cgatcgcctc cccttcgatc cggagaaggt ggcgtactgg
     2281 tgcccggtcg acttctacag cggcggcgtg gagcacgcca tcctgcacct gatctactcg
     2341 cgctttttct gccgggtgtt ccgggatctc gggatggtgc agcacgacga gccgttcacc
     2401 cggctgctca cgcaggggat ggtgctcgcg accgacggca gcgcgatgtc gaagtcgaag
     2461 gggaatacgg tcgatccgga cgacctgatc gagaggtacg gcgccgacgc ggttcgcctg
     2521 tacgtcatgt tcatggcgcc tcccgagaag gaggtggcgt ggtcggacgc cggcgtcgag
     2581 gggagtgcgc ggttcctcgc gcgcgtctgg cggctggtgg accactggag cgacgcggtg
     2641 gccggggcgc cggccgactt cagccggctc gacctgaccg aggcggaaca gggcctgcgc
     2701 cgggcggcgc acgacacgat ccgccgcgtc acgcacgacc tcgacccgcg cgtccacctg
     2761 aacaccgccg tctcggcgct gatggagctg gtgaacgccc tgtacgcgtt ctccgaggaa
     2821 cagaccggcc tgggcgtccc ggggcggcga gcggcgggca ctgaggttcg tgcggccgag
     2881 cgccccgagg cgctcgcggt gctgcgagag gcgctcgacg cgctggtgct gatgctcgcg
     2941 cccttcgcgc cgcacaccgc cgaggagctg tgggagatgc tcggccaccg cgacggcctc
     3001 gcctgcgccg cctggccctc ctacgatccc gtcgtcgcgc ggtcgtcgga gatcgtcgtc
     3061 cccgtgcagg tgaacgggaa gctgcgcgga cgcctgacgg tgcccgccga catcggcgag
     3121 gacgccctgc gcgaggccgc gctcgccgag gcctcggtgc gcgtgcacac cgccggcaag
     3181 acggtgcaga aggtgatcgt cgcgcgcggc aagctggtga acgtggtggc gcggtgaggc
     3241 gggtctgccg cgggccggcg gaggtcggtg ggctgaagcg gacgtctggg ggaggactgg
     3301 gatcgtgaac cgctttgcac gacttcgaac agccgccgtc gtcctcgccg cgtgcgtgtc
     3361 cgcgtcgtgc gggtacgccc tcgccggccg tggctcgttc ctgcccgact cgatccaggt
     3421 catcggcatc cctaacttcg gcaaccgcac gccttacctc gaggtggagc aggtcctcac
     3481 ccagcgcgtg cgctccgagt tcatcggccg cggcaagtac aaggtgctgc cgcaggacac
     3541 cggcgtggac gccgtcctgg tggccgagat caccaacatc tcgatcgcgc cggcgagctt
     3601 caacgaggac cagcaggcca gccggtacac gatcagcgtc gtggtctccg cgcgcctggt
     3661 ggaggcgggc accgacaagg tgctgtggca gaacccgagc caggtctttc gtgaggaata
     3721 cgcgctgtcg agcggcggcg agagcgtgct cgatccggcc gccttcttcg gccagtcgtc
     3781 caacgcggtc gaccgcctgg cggccgactt cgcgcaggcc ctggtgacgg cgatcctcga
     3841 ggcgttctag gggctagggg gggggggggg ggggcggggg gctgggggcg gggggcgggg
     3901 ggctgggggc tgggggctgg gttcgcggat ggcaaggggc acactgacct cggcggcgtt
     3961 tcggcgccag atcgcctcag gcgagctggg gcctctctac ctggtgaccg gccccgacga
     4021 cgtggagatg agccggctgg cggccgccct caccgagtcg gttcccgagg acttccgcgc
     4081 cttcaacgtc caccgcttcc acggtaccga caaggtcacg ctggcgcagg ttctcgacgc
     4141 ggccagcgcc tatccgctgc tcgcgccgcg ccgcgtggtg ctgctgctgc aggccgggcg
     4201 cgtgctcggg aagaagggcc aggccgacga cgagccggag gaggccgggc cggacgacga
     4261 gaccgtgggg gaggccgttg agcggcccgg cgcgccccga aagacgggcg ggcccgcgcc
     4321 gctgcaggtg ctcaagcagt acgcggcggc gccgcacgat cacgcgacgg ttgccatctt
     4381 cgccccgggc ctgcagcgtg cgttcgagga catcggcggc cgcgccgtga tggtcttctg
     4441 cgaacccgac caggacccga tcaacgacct ggccgccgag cacggcgtcc gcttccaccc
     4501 tgcggccgca cagttgctcc gccagcgggc gggcgacgac ctggaacggc tgaaggccga
     4561 cgcggaacgg gttcttcttt acgcggccgg ctcccaggtg attacgcggg aacaggtgga
     4621 ggcggtttcg gggcggcagg ccgccgcggg cggacgtgcg ctctggggcg cggtggcggg
     4681 cgggcggacg gccgaggccc ttcgcgagct gaacctcgag atggccgagg gggccgtgcc
     4741 cttcatggtg ctggggctcg tgcgatcggc ggtggagcgg accgtgagcg ggccggcgct
     4801 ggcggcggcc tacgacgcgc tcctgcggac ggacctggcg ctgaagagct cgggcggcga
     4861 cccgcaggtc ctgctggaac gcctggttgt ggagatgtgc tcgctgaccc agtagaacag
     4921 gcgtccccgc ctgttcccac aacaggcgtc cccgcctgtt cccacaacag gcgtcccgcc
     4981 tgttctcaca acaggcgtcc ccgcttgttc cgaggcgcgg tcttggcgtg agcggggccg
     5041 gaaggccgcc cgccagggcg tttcatccgg cctgcggagc gtttgctacg ccgaaagctt
     5101 gttgatgccg acctggagcc gcgacttgta gcgggcggcg gcattgcggt ggatgatgcc
     5161 cttgcccgcc atgcggtcga tgagggcgac ggtcttcgag agcgccccgc gggccgtgtc
     5221 ggcgtccttg gcggcgaccg aggcacggat gctcttgatc gcggtccgga gctgcgagcg
     5281 gaaccggcgg ttctgttccc ggcgcttgag gctctggcgg tgggccttca gtgctgaagc
     5341 gtggctggcc acagtcgttc ttcccctgta atatctgcgc ggcacgccgg tcgcgtgggg
     5401 cgccggcaca aacgcctatg atagcatagg cggcccggcc gtaggggccg tccacgaccc
     5461 cggctgccgg cggcgccgct gcccgggcgc aagccctact gtatccgccg cgagcgtccg
     5521 aaacgtcctg tgaggggccg ccgcgccggc tggccccgga aagcggcatc gtgtcgatct
     5581 cgatcgtggg cggtctcgag gacatcatgt cggcgatgga ggtgcgctgc ccggcggtgg
     5641 ccgctcactg ccgccgcgtg tctctctact cggtccgcct cgccacccag tatggcctcg
     5701 acaccgagac ggtcgaggcc atccgggtgg gcgcgctgct ccacgacctc ggcaagctcg
     5761 aggtgccgcc gcggctgctc gacaagcccg gccggctcac cgagcgcgag tgggaccgcc
     5821 tgcgccggca tcccgaggcc ggtctcgcgc tggtcgatcg cctcggcttc gacgctccag
     5881 tgggcgagat cgtcctctac caccacgagc ggttcgacgg cagcggctat cccgacagcc
     5941 tggccgccga gcgcatcccc tggcccgttc gaatcgtcag cgtgatggac gccttcgacg
     6001 cgttgaccag tccgcgcccg taccgcgcgg cgctctcgct cgacgcggcc cgctcgctgc
     6061 tcgcccgcga ggcgggcacc cgctactgcc cctgggtggt ggcgggcctt ctctcgctgc
     6121 cgcggccgct gctccacggc gtcgcctccg agccgccgca ccagtgccgt cccgacggct
     6181 gccccagccc cgccgcgctc ctgaaggcga cccaggcgtg gaaccgccat gccgcgctcg
     6241 ccgatcacgg ctgcctctcc gcgaccgcct gagcggaatc tcccggccga cggcttccgg
     6301 ccaggcctgc gcccgcgcta agatgccact ttcgccatcg gagcggcatt cttcccaagg
     6361 tgtactacat gacgagcact cggcgggtgc ggtgggcgac cctcctcttc cttctcgcgg
     6421 gcggcgtttc gatcggcgcg caggagccgc ccgcggccgc ccgcccggcg ccggcgaccc
     6481 gtgcggtaca gcaggagcgg atcgacgccg cctcggtgcg ggccgccgag gggctcgaca
     6541 cgttcctgcc ccggcggacc gactcgatcc tccgcgcgct ggcgtcgagg ttcgaaccgt
     6601 cacgcgcgat ggacctggtt tccttcatgg atcggttctg gcgggtgccg ggcaacagcg
     6661 gctataacgc ctcgctcgag cggatcaggt cagggctgac cgcggccggc ttcgctgaag
     6721 ccaaggggcc gggcgcctcg tcgccgagcc tgtggttcga ggagtacccg aacgggggga
     6781 acggctggga gcccgtctcc ttcgcgatga cgatcctcga aggcgaggcg ggtccggccg
     6841 agcgggtctt cgaccccgtc atcgactaca tcgcgctctg catgaactcg ttcccgacgc
     6901 cccccggcgg cctcaccgcg cgcctcgtat acgtcggcga cggaaacaag cccgaggact
     6961 acgcctcggt ggacgtcaag ggagccgtcg tcctggcgga cggcggtatg cggcgcgtgt
     7021 ggcaggaagc cgtccgggcc aggggcgccg ccggcgtcgt ctcggcggaa cggccgcccg
     7081 actatgcgag gccggacgag acgcccgaga tcttccagtg gggcggcatc ccgtacgacg
     7141 agaaggcgcg ggccttcggg ttcaaggcga gcccgaaggt ggcgggccgg ctgaaagagc
     7201 ggctgaagaa agggccggtg accgtcaggg tcgagatcga gacgaggttc gacccctcgc
     7261 cgggccggat gctggtcgcc gagatcccgg gcgccacccg gcctgccgag cggatcgtga
     7321 tggtggcgca cgtgcaggag cccggggcga acgacaacgc cacagggtgc gcgacgctgc
     7381 tcgagctggc gcgggcggcc gctgccgcca ctgcctcgaa ggcgttgccg aggcccgggc
     7441 gcacgctgac cttcgtgtgg ggcgacgaga tgcgcgcgag ccgcgagtgg ctgcgcgcgg
     7501 aaccggggcg ggccgcgtcg gcccgcgcca tgatctcgct cgacatgacg ggcgaggacg
     7561 ccgcgaagac cggcggcacc ttcatcatcg agaaggagcc cgacccgtcg gctgtctggc
     7621 cgcgcccctc cgacccgcac accgagtggt ggggcggcgg cgcctaccaa cgggcgctga
     7681 aggggagcta cctgaacgac ctgctgctgg ccgtctgcct gaggcgcgcc cgcgagaccg
     7741 gctgggtggt gcagaccaac ccgtacgaag gggggagcga ccacagcatc ttcctcaacg
     7801 cgggggttcc ggcgctgctc gtcagccatt tcaccgaccg ctactaccac acgaacctcg
     7861 atcggcccga caaggtgagc gcaaccgaga tggcgcacgt ggggatctcg gtcggcaccg
     7921 ccgtgatgct gatcgcgtcg gcaggcgacg aggaggcgcg ggcggttgcc gacctggtgg
     7981 ccgacgcggc gcgccgccgg ctggagctcg aggcgcggca gtcggccgag ctgatcgccg
     8041 gcgcggccga ccggaccgct gccgaggctg gtgagcgggt gctgcacgac gggtggcggg
     8101 cgtggtacgc gagcgcgctc gagagcgttc tcacgctgcc gcaggcgccg gctgggcgcg
     8161 cgctccgcgc ccgcatcagg acggcgatcg acgcgatggg cgccgccgcg gcgggagccg
     8221 cgcgatgatc gaggtcgagg ggctcaccaa ggtctacggc cgcctcaccg cggtggatcg
     8281 cctctcgttc ggcgtccgcg cgggcgaggt ggtcggcctg atcggcccca atggcgccgg
     8341 caagacgtcc acgctgcggt gcctggccgg catccaggcg cccagcgccg gcaccgcccg
     8401 catcgccggc ttcgacatcg tctcgcagcc gatcgaggcg aagcgccgcc tggcgttcat
     8461 gcccgacgag ccgcagctgt tcgagtacct gaccgtggcc gagcacctga ggctggtcgg
     8521 gcgcctctac ggcgtgccgc gcgtggacga gcgcagcggc acgctgctcg aggagctgga
     8581 cctggcgggg aagcagcacg cgctgccggg cgagctgtcg cgcgggatga agcagaagct
     8641 gatgatcgcg tgcggcctgc tgcacgaccc gcaggcgctg ctcttcgacg agccgctcac
     8701 ggggctcgac ccgatcggca tcaggcggat gaaggagacg atcgtggcgc gcgcgcgcaa
     8761 cggcgcggcg gtgctcgtct cgtcgcacct gctgcacctg gtcgaggaga tctgctcgcg
     8821 catcgtgatc atcaacaagg gaacggcgat cgcggacggg accctcgagg agctggccgc
     8881 gcgcgccgac ctcacggccg actccgacct cgaggacgtc ttcctcaagg cgacgggcca
     8941 cgaccggggc ggccgggcga cgtgacctgg gccttcctct acctgaccgc ccgcagcctc
     9001 cgcaaccgcg tcgcggcgcg cctgaggcgc ttgcggcagc cgcgctacct cgccggcctg
     9061 gtcgccggcc tcgcgtacct ctacttgttc atcctgcgcc accaggtgcg cgccgtcagg
     9121 cgcggcgact tcacggtgga tccgcagttt gccagcatcg ctccggccgt gctcgtggcg
     9181 gggggcctcc tgctctgggt ggtgtcgctg gccgcctggc tctggccctc caccgagccg
     9241 cccctgcgct tcagccggcc cgaggtgcag ttcttcttca ccgcgccggt gagccgccgg
     9301 cagctcgtgc actacaagct cctgcgcgcg cagctcggca tcgtgttcgg cctcgcgatc
     9361 gtgtccctct tctcgggcgc cgccgtctcg gggcggatct ggttcctgct cggaggctgg
     9421 ctgctgtttg cgaccgtgcg cctgcacctg gtcggggtgg cgttcacgcg cgcgttcacg
     9481 cgcgcgagca tcgcccggcg gcgcggccgc ccgctccgct cggcctggcc cgcgctggcc
     9541 gcgattggcg cgttgtcggc cgcgatcctg ggaacctggc tggcgaacct gcccgccctc
     9601 gtctcgctcc cgttcggcgc ggccatgcgg cgggtcttcg acgttttctc gagcggtccc
     9661 gcggctgccg ctctctggcc cttctcggcg ctgatcgccc cggtactggc caccaacacc
     9721 cggcagttcc tcgcctcggc atggcccgcg gcgctgctgc tggcgctcaa ctactggtgg
     9781 gtgctgcggt cggagacgac gctggaggag gctgccgggg cggcggaacg cgagcaggcc
     9841 ggcgggcgcc ggcaggcgcc gaagcccgtc acccgcaggg cccccttcgc gctcgcgccg
     9901 tcgggacggg ccgaggtggc catcctctgg aagaacctga ttctcggcgg ccgctacctg
     9961 acgccgcgcg tcgtgcttcg cctcgtcgtg ccgcttctcg tgctcgccgc ggccgcggcc
    10021 gtcggggagt tcgcccttgg cgtcgcgccg gccgcgctca tcgtcgccgg cgcgctcacg
    10081 ctgctcggcc cctacatggt ccgatacgac ctgcggcacg acctgccgcg gctcaccgtc
    10141 ctcaagacgt ggcccgtccg cggcgcgacg ctggtgcgcg gcgaggtgct cgccccggcg
    10201 ctcgtgctga cctccggcgt ctggatcatg ctggccatcg ccctggtgct ggccgagggc
    10261 ctcagctacg ggggactctc gcttgccgcc cgcgtctggc tggggctggc cgcggccttc
    10321 gcggccccct cgttcctgct cgcgcagctc gtgatccaga acgcggccgt catcgtcttt
    10381 cccgggtgga tcccgaccgg ctcgaaccgc ccgcggggcg tcgaggcgat ggggcagcag
    10441 atgctgatgt tcgccggcac gctgctgctc atgatcctcg gcgttctgcc cgccgcggcc
    10501 gttgcggggg ccgccggctt cgtcctcttc caggtgatcg gctgggccgc cgtcgcggcc
    10561 gcggcgctgg tgttctcggc cgcgctcgcc gccgagagcc tgctggccat cgagctgctc
    10621 ggacgcctga tcgagcgcac cgacccggtg gacgtggaag cggaagaacc ggtgtaggcg
    10681 aacacgagga gaaagacgcc cgggggcgac atatggattc ggtccctccg attcccgccg
    10741 ccccgatccc aaccccgccc ctgcgccttc cggcgggggc tatagaatcc cgccgcgacg
    10801 gccgataccc gatgtgaggt tgtccaaccg tgcctcgagg tctgcccatc gtcctgttcg
    10861 gcctggcgat tgtctcgggg gtggccgctc ccgcctcggc ccagatctac agctaccgcg
    10921 acgcgtccgg cagcctggtg ctctccgacg cggcgccgtc tgatccctcg ctgtcggttc
    10981 ggaccttcga ggcggtgaac accagcgagc gggtgcgcgt gacgcgccct gtctcgaagg
    11041 cgtaccggga gcagttcgac gagctgatcg tgcactacgc cgaggcacag gggatccgcc
    11101 ccgacctggt gcgtgcggtg gtgcaggtcg agtcgggcta caacccgcgc gcgctctcgc
    11161 ccaagggggc gatgggcctg atgcagctga tgcccgacac cgcggccgca ctcggcgtca
    11221 agcgtccatt cgaccccgag gagaacgtcc gcgggggagt cgcctacctt cgccacctgc
    11281 tcgaccgctt caacggcaac gaggagctgg cgctcgccgc ctacaatgcc gggcccgcag
    11341 ccgtggggcg ctacggcaac cgcatcccgc cttaccgtga aacccgcgac tacgtgcgca
    11401 aggtgcgcac gcggaccacc gtctcggccg ctgccgcgaa acccaagctg gtgatctaca
    11461 agtgggtgga gctggtggaa gggaagccgg tgacgcggta ctccgactca ccgccggcga
    11521 ccgggagtta cgagagggtg ccggggggaa ggtgaggggg gggtgttggt tttagttgtt
    11581 agttgttagt tcttagttct tagttgccgg gtctcgcgga gcgagggaag ggatgcagga
    11641 tcaccggcag ctggagattt ggaagcggtc gatggattac gtggtggagg tgtaccagtg
    11701 gtgccttcag ctgccgaagg atgagcggta caacctcact gaccagttga agagggcctg
    11761 cacgtcggtc ccactcaaca ttgcagaagg cgcggcctgc gaaacgaacg gcgatttcgc
    11821 ccggttcctc ggctattcgt acagatcatt gaaagaaagc gtcacctgcc tcgagctgtg
    11881 tgaacggctc tacccgtctc tttccaacgc tccgacctgc ctcatcaacg aggccgagca
    11941 gctggcgaag atgacgcgca cgctcatcca gcggctcaag ccgcccttca tccaggaaac
    12001 ctgacacgcg tcagcgtgca gcctcgggac gaaaggacca aaaaccaaaa actaaagact
    12061 aagaactaag aactaagaac taagaactaa gaactaagaa ccaagaacta agaactaaga
    12121 accaagaacc aaaaactacc cccttctcat ctcctccacc acccccctcg cctcctccaa
    12181 catccgctcg atccccgacg ggtccttgcc gccggcctct gcgaagtcgg ggcggccgcc
    12241 tccgccgccg cccaccatcg gtgcgaggcg cttgacgatc tgcccggcgt ggatcttcgg
    12301 caccaggtcc ttggtgaccg acacgacgat cgcaacacgg ccgtcattct cggacgcgag
    12361 caccacgacg ccgctcttga cctggttctt gagcgagtcg gccatctggc cgaggccggc
    12421 cttgtcgagg ccctgcacgc ggcgggcgac gagcgtgatg ccgcccgcgt cggcctgccc
    12481 ggccggtgtg gccgcagtcg ccggggcccc cgccaggctg gccgtcgcca gctgcacctt
    12541 cagctggctg ttctcgcgcg acagccgctt gaggtcggcc tgcagccgct cgatcgcgcc
    12601 cgccgcctgg tcggccggca ccttgagcgc ctcgaggacg ccggcgagcg tcctgcgctg
    12661 ctcgcggtag aactgcagcg ccccgtggcc cgtcaacgcc tcgatccggc gctggccggc
    12721 cgcgacgccg ctctcgtgga cgatgacgaa gcagccgatg tcgccggtgg cggcgacgtg
    12781 ggtgccgccg cacagttcct ggctgaagcc gggtaccgag accacgcgga cgcggtcgcc
    12841 gtacttctcg ccgaacagcg ccatcgcgcc ggcggcaatc gcctcctcgg tggcgcgcac
    12901 ctcggtttcg accggcgtgt tgcggaggat ctcggcgttg accagccgct cgatctcggc
    12961 cagctgctcg gaggtgaccg ggtcggggtt cacgaagtcg aagcggaggc gatcgggcgc
    13021 gacgagcgac ccggcctgct tcacgtgcga cccgagcacc tggcggagtg cggcgtgcag
    13081 caggtgcgtc gcggtgtggt tccgccgggt ggcgttgcgc agctcctcgt ccacctcggc
    13141 gacgagggtg tcgcggtcgc tgatggtgcc gctctcgacc acgacctcgt ggaggcgtgg
    13201 gcggtctgcg ccaacgcgca cgagcccctc gaccctcgct ctcgctcccg acgtgctggt
    13261 gatcgcgccg gtgtccgaca cctggccgcc ggcctcggcg tagaacggcg tccggtcgag
    13321 cgcgaggtag ccgccctggc cctcccgcag ctccggcacc gggcggccgt cctcgtcgag
    13381 cagtgcgatc accgccaggt tgtcgaggcg ggtggtccgg tatccctcga actggtcggg
    13441 ccgcccgcga agcgtttcgg tggccgcctc gagcgcggcg cttccggcgg tggcgaggcc
    13501 gaacgcgtgg cgggcgcgcg ccttttcctt ctgaccctcc atcgcgcgct cgaagccggc
    13561 gcgatcgacg gccaccccac gctgcccggc catgtcctcg atgaagtccc tcggcactcc
    13621 gaaggtgtcg tagagccgga acgcctcgtc gccggcgagc acgccgcccg cggccgcggc
    13681 ccgctcgagg ctgtcctcga gcttgggcag gccgatccgg agcaccgact cgaagcgctc
    13741 ctcctcggcg tggatcatcc gctcgacgta ctcgcgctgc tcgcgcagct cggggtacgc
    13801 gtctcccatc tcgccggcca ggacggcgac gagacggtgg aggaacggct cggtcatccc
    13861 gaggtgcttc ccgtgggtca tcgcgcggcg catgatccgc cggagcgtgt agccgcggaa
    13921 ctcgttggac ggcaccaccc cgtcggcgat gaggaaggtg gccgcccgcg cgtggtcggc
    13981 gatgacgcgc atggagacgt cgcgcggctc catcgacccg ccgtagcgcg cccccgcgag
    14041 cgccccgatg gcgccgagca gcggcgtgaa caggtcggtg tcgtagttgg agaggacgcc
    14101 ctgcagcacc gcggtgatcc gctcgaggcc catgccggtg tcgatcgagg gggcgggcag
    14161 cgggttcagc gtcccgtcgg gctgacggtc gaactccatg aacacgttgt tccagatctc
    14221 gacgtagcgg tcgcacgaac aggccacccc gaggcagacc ggctcggggc agggcaggtg
    14281 gtcgccgcgg aagtagtgca cctccgagca gcggccgcac ggcccggtgt cgcccatcgc
    14341 ccagaagttg tcctcgccga gctcgctgat ccgctcggcc ggcaggtact tccgccagat
    14401 cgcgtgggcg tcctcgtcgc gcggcacctg cgggaccttg tcccagtggc ggaagatcgt
    14461 ggcgtggagg cgctcggggg gcagctgcca cacctcggtc agcagggtcc aggcgaaggc
    14521 gatcgcgtcg gtcttgaagt agtcgccgaa ggagaaattg ccgagcatct cgaagaacgt
    14581 gtggtggcgc ggcgagggcc ccacgttttc gaggtcgttg tgcttgccgc tcacccgcat
    14641 gcacttctgg gacgaggccg cccgcgtgta ctcgcgccgc tcccggccga ggaagacgtc
    14701 cttgaactgg ttcatcccgg cgttggtgaa gagcagggtc gggtcgttgg ccgggaccag
    14761 cggcgagctc gccaccacgc gatggccgtt ccgggcgaag aagtcgaggt aagaggcgcg
    14821 gatctccgag gaagtcataa caggacattg tagcttacgc ctgtcgtgca ggaggtgcag
    14881 ggggtgctga gggtgctggg ggtgcgagtg ctggggtgct gaaggtgcta aaggtgcggt
    14941 gctgagggtg ctaaaggtgc ggtgctgggg tgctgagggt gctaaaggtg cggtgctggg
    15001 gtgcggaggg tgcgacctat tcctgctcca gctctcccct ccagtgtctc ttgagcgccc
    15061 gggtgacggc tgacggcgcg aagccctggc ggacgagctg ctggtgcagg cggcggaagt
    15121 gggcggcatc gcggatgcgg gcggccgggc cgcgcaggcg gcgcgcgagc gccgcctcga
    15181 gcagcgtgtc ctcgtcgatc gcaccgaaca cctcgtctgc cgcctcggcg gccacctcgg
    15241 gggcaatccc gagcgcgtgc agccgctgca gcacgcgcag gcgcccgcgc gagcggacct
    15301 gcgcctcgac ccgcgccgcc gcccgcgcca cccggcggtc gtcgagcagc cccgacgcgc
    15361 gcagccgggt cacggcctcc tcgatggcgg ttggggggtg ctcgcggcgc tcgagccgct
    15421 cgcgcagctg ccgctcggaa agctcgcgcc gcgccagcat ggccagcgcg gccgagtacg
    15481 cggggttgga cactctttcc cgtctactga cggaaccgct gcacctcggg cgcggcggcc
    15541 gggccgccgc cgaaccccgc ccgcgccatc tcgtcgcgcg ccgcgtcgga ggtcatcgcg
    15601 attccctgca ccttcagctt gaactcgtcg acgttggtcg cccagtgcag cgcctcctcg
    15661 tacgagacga tcccctgctc gtagagcgag aagatcgact ggtcgaaggt ctgcatgccg
    15721 tactgcgacg tgccggccgc gatcgcgccc gggatcaggt gcgtcttctc cttgtccacc
    15781 acgcagtcgc ggatgaacgg cgtcgtgacc atcacctcga cggcggcgca ccgccccagg
    15841 ccgtcggccc gcgggatcag gcgctgcgag atgaccgcct tcagcacgct ggcgagctgc
    15901 agccgcacct gcttctgctg gtgcgggggg aagacggcga tgatgcggtt gatcgtctcg
    15961 gtcgcgtcga gggtgtggag cgtcgagaag accaggtggc ccgtctcggc cgcgaccagc
    16021 gccgtctcga tcgtctcgaa gtcgcgcatc tcgccgacca ggatcacgtc ggggtcctgg
    16081 cgcagtgcgc tgcgcagcgc ctgcgcgaac gagtgggtgt ccaccgcgac ctcgcgctgg
    16141 ttcacgatcg accgcttgtc gcggtgcagg aactcgatcg ggtcctcgat ggtcaccacg
    16201 tgcgtgcagc gcgtgttgtt gatgtgatcc accatcgccg ccagcgtggt gctcttgccc
    16261 gacccggtcg tccccgtgca gagcaccagg ccgcggtcct cgtcggcgat gcgcgccagc
    16321 accgccggca gcccaagctc gtcgatcgtc cggatcccga acgggatcac gcgcagcacg
    16381 atcccgatgg tgccccgctg ctggaacacg ttgcagcgga accgccccag cccctgcacg
    16441 ctgtaggcga ggtccacctc ctgggcttcc ttgaacttct gccgctgggc cgccgacatc
    16501 accgaggcgg ccatcgtgac cgtgtcctcg tgcgtgagcc gcttctcctc cgaggcgggg
    16561 gtcagcatgc cggccacccg catcatcggg tagctgccga ccttgaggtg gaggtcggac
    16621 gctccgctct cgacggcgat cttgagtaaa tcgttgacgt gcatggggtt atggctcggc
    16681 agcagcacgc cggccaaccc ggcggcccgt taccgggtga cggcgttctc ctgcgtggtc
    16741 ttatcggccg atcccttgcc cggatacacg tacgtcagcc agccgtgccg gtcgggcagg
    16801 acgccgttga tggtgtcgaa gaacgcctgc tgcagacgct tggtcaccgg gccgcagcgg
    16861 ccctcgccaa tcacgatctt gtcgaccgac cggatcggcg tgacctcggc ggccgtgccg
    16921 acgaagaaca gctcgtcggc gatgtagagc gcctcgcgcg ggatgacttc ctcgcggatc
    16981 gggtagccga gatcgcgcgc cagcgtcagg atcgagtcgc gggtgatgcc tggcagcacc
    17041 gacgactgca gcggtggcgt gtagaggata ccgtcgcgga ccacgaacag gttctcgccg
    17101 ctgccctcgc tgacgtagcc gaaggtgtcg agcgcaatcc cttccgtgta cccctcgacg
    17161 accgcctcca tcttgatgag ctgtccgctc gcgtagttcg cgctcgactt ggccagcgtc
    17221 gggaaggtgt cgggcgcgct gcgccgccac gagctgaccc ggacgtccac tccctgctcg
    17281 agcgcctcgg ggccgaggta cttgccccac tcccacacca ggatggctgc ctcgacgggc
    17341 gagccgagcg ggttcacgcc cggggcgccg tacccgcggt agatcagcgg gcggatgtag
    17401 caggcgcggt agttgttcgc gcggatcgtg tcgagcaccg cctcgccgaa ctcctcgagg
    17461 ccggcgcggt actccatccg gtagatcttg gccgaattgt agaggcggcg caggtgcgca
    17521 tcgagcctga agcaggccgg gcccttcggc gtctcgtagc agcgcgcgcc ctcgaacacc
    17581 ccgctcccgt agtggatcac gtgggacgcg atgtgtatgg tggcgtcctt ccatgcgacg
    17641 agcgacccgt tcatccagat cgtgccggtt tccggaaagg ccattctgtc acctcgagtg
    17701 tgggagttgg tgcggatcgc cggggatgat gtcggagggc ctcgggaatc ttagcattca
    17761 ctgcgcttcg ccacaaaacg ggcagcggtc ggcgcgcggg tgcagcggct tgcggcactg
    17821 ccagcagaac cgcggcgagg gcgcctcgcg cgcgcgcagc cgcccctgga tctcctgcag
    17881 ctgcgagctg gccggccccg ggcgcaccgg cgtcaccggc ggacgccccg cccgcgacga
    17941 cacccacttg ctgcgggccg ccagcaggtc gtcgagcagc tcgccggcgc gctggtagcg
    18001 atcgttgacg gcgggcgcca tcgcgcgcat gacgatctcg ttcagctccc tggggatccg
    18061 gctgttccgc gcgcgcggcg gctggaacag ctcgccccgc atcagcttct ggagcgcggc
    18121 cggcgacggc gtgtcgtagg gaaggctccc ggtgatcatc tggtacatcg tcacgccgag
    18181 cgagtagatg tcggaggcaa agaccgcctt cccctggaac tgctcgggcg ccatgtaggg
    18241 cgggctgccg atcaccgtgg ttccctgcga ggcgatctcc aggaaccgcg cggtgccgaa
    18301 gtcggccacc ttggccatgc cggcgtccga taccagcacg ttggccggcc gcaggtcgcg
    18361 gtggagcacc ccctgcgtgt gcgcgtgatc cagcgcgttg cagatctggc aggtgaagtc
    18421 gagcgcccgc gtcagctcca gcgcgcccgt ccggccgatg atgttctcca gcgtctcgcc
    18481 ctgcacgtac tccatgacga tgaagaacac gttctcctgc ttctcggccg tcaggatcga
    18541 aacgatgttg gggtggctca gcgacgcgag caggcggggt tccttgatca gctcgccgaa
    18601 gtcgatgttc tgccggtgcg ggaccttcag cgcgaccttc ttctggatcc aggtgtcctc
    18661 cgccaggtac accgccccga agccgccgct gccgagcgcc gagaggatct ggtacttccc
    18721 gatgatctga ccgcgaaaga acatcgcgcc ggagtatacc ccacgcgtga gccgtgaaag
    18781 cgctcgctgc gctcgcggct tcggcgcggc aagcccttga gccgtgagcc ttgagccttc
    18841 tacaggtctc cccagagata ctgcagcacc gggatcgcga cggtggcggc agcatcggcg
    18901 cggagtgtgc gggtgccgag cgtcaggcgc gaccaccccg acgaggcggc cagcgacacc
    18961 tcggccgccg tccacccgcc ttccggcccc acgaggatcg cggccgagac gggccctggc
    19021 agcgagggca gatcgagcgc tgccggccca tcgacagcgg gctcggcgag catcagccga
    19081 atcggatccc ccagccccgc caccgcctcc tcgaaggtca ccggctcgcg gacctcggga
    19141 accaccgcgc ggccgcactg cttggccgac gacaccgcga tccgctgcca ccgttcgtgg
    19201 cgacgcgctt tcagcagccg ggcgcgatcg gcctccacgc gcgtcgagac gaccgggacg
    19261 atcgcccgca cccccagcat cacggcgtcg cgaaccacct cgtccatgcc gtcgcccttc
    19321 agcacggcct gcgcaagcgt cagcgcgacg cgcggctccg ggcacgcgca caccgagccc
    19381 accacgcgca gcgtcaatcc tgccggcgcg gggttctcga ccaccgcctc gcactcgtac
    19441 ccgcgcccgt tgaagagccg gacctcggcc ccgcggcgca cccgcaggac gcgcaccgcg
    19501 tggccgctct cctcggcgga gagcactgcc gaacgatcgc ccggctcgag gcccggggcg
    19561 tggaaacgca cgtgcatcgg ggaggaacct ggggggcgga aaacggggaa cggaagacgg
    19621 agcacgcaaa cggacaaccg aaaatgctca gcgccgatcg aagcggaggc ccacccactc
    19681 gtcctcgacg aggcgggaaa ccggtgtcat caactgcgcg ccgagcgccg cggtcaccgc
    19741 ctcttcctcg tcgagcgtga ggccgctcgc gatcaggctg ccgcccgggg cgaccgcgcc
    19801 cgcgagcgcc tcagcctgac cgatcagcag cgcaccggtc aagttcgcga gcacgaggtc
    19861 ggcggccagg ccggccatgc cccggaagtc gccgtggcgg agcgtcacgg cctccgccgc
    19921 gccgttcagc ccgaggttgt cggccgccgc ggcgatggca tccgcatcgt cgtccagggc
    19981 gacgaccgac gccgcgccga ggcgagcggc ggcgatggca agcacgcccg agcccgtgcc
    20041 gacgtcgagc acgtgaaggc ccgaagggtt gcgagcctgg agcagcgcca ggcacagcct
    20101 cgttgtctgg tggtggccgg tgccgaagcc catcgacgga aggatcgtca cgaccaggtc
    20161 gccatcctgc gccggcggcg tcagccaggg aggggtcacg agcacccggc ccacgcggac
    20221 ggggcccagc ttctcctggc tgcggcgcgc ccagtcttcg tcggggacct caatcggcct
    20281 cggttcgaca acagggccga ggcccgcgag cgcgcgaccc gcggcgtctc ttccggcgtc
    20341 gctggcgaag aagacccgcc agcctgcgtc ctgctcctcg atggcggtcg ggttgagatc
    20401 gtcgagcgcc gccgcgagaa gatcggccgt ctcggcgcgc gcctcgggcg ttacggcggc
    20461 gaagcggacg tcgagcgcgg gccaggcctt gggcatcggg tcagctgaag atgtccttca
    20521 cgcggtcgaa gacgctcttc tcctcgtcgg cgtcgccctt gggcgccttc accttctcga
    20581 tcggcatggc cttggagagc tgctcgagca gggtgcgctg ctcgcgcgtg agcttcttcg
    20641 gcgtcaccac ctgcacgacg acgtgcaggt cgccgcggcc gcgcccgttc accgagggca
    20701 tgcccttgcc ccgcacccgg aagacgtggc cgctctgcgt gccggcggag atctcgaggg
    20761 gctcggtgcc gtcgagcgtc gggatctcga tggttccgcc gagcgccacg accgggaatg
    20821 tcaccggcac gcggcacacc aggtcgtcgc cctcgcggcg gaacacgggg tggtccttca
    20881 cgtgcacgac cacgtagagg tcgccgggcg ggccgccggc cacacccccc tccccctcgc
    20941 cgtagaggcg aaggcgctgg ccgctggcga tgccggctgg gatgcgcacg gcgagcttcc
    21001 gctcgcgcgc cacctggccg gcgccgcggc agtcggagca ggggcgggtg atgattcggc
    21061 ccgagccgcc gcactgccgg caggtctgcg cgacggtgaa gaaaccctgc tggtagcgga
    21121 tctgcccgcg tcccccgcag tgcgggcaga ccgacgggct gctgccgggt gccgcccccg
    21181 taccgcggca cgtctcgcag gtctcctggc gggggagctg gatggtggtc tcgagcccgc
    21241 gcgccgattc ctcgaactcg atctccaggt cgtaccgcag gtcggcgccc cgctgcgggc
    21301 cgccgcgccg cccgccgccc atcccgaaga tgtcgccgaa gatgtcgcgc aggccgccga
    21361 agatgtcgtc gccgaagccc tggaagatcg aggggtcgaa gccgccggcc cccgcgccgg
    21421 cgacgccggc gtggccgaag cggtcgtagg ccgcccgctt gtcggcgtcg gcgagaaccg
    21481 agtaggcctc ggccgcctcc ttgaacttct cctcggcgtc cttgttgccc gggttgcgat
    21541 cggggtggaa cttcagcgcg agcttgcggt acgcgctctt gatctcctgg tcggtcgcct
    21601 cgcggccgac cccgagcacc tcgtagtaat cgcgcttgct cacgccttgg ctacccttac
    21661 cacggcggga cgcagcagcc ggtccccgtg cacgtacccg cgacggatct cctcgaccac
    21721 ttcgccgtcg cggctccccg gccgctcctc gtacgtgatc gcttcatgga agcggggatc
    21781 gaaggacgag ccgagcgcct cgatcggctt gacgccccgc ttcgcgagga agtcgaccag
    21841 ctgcttgtga atcagctcga taccctggcg gtaggcctcg gccgagccgc cggccggggc
    21901 ctgcagcgcc cgctcgaagt cgtcgaggat gggcagcagc ccctcgaaca cctcggacgc
    21961 ggcgtactga accaactctc gccgctcccg ctccacgcgc tttcgatagt tgtcgaactc
    22021 ggcgctcttg cgcagcagca agtcccggta ctcgtcgcgc tcccgctcga gggccgccag
    22081 gtcaggttcg gcggcggccc cactctcgac ccgttcctcg gccggggcgg cctcctctaa
    22141 aggccgcgcc gccgtgtcgt cgcgttgctc catgaatccc tagtctcccg tgccgccctg
    22201 ttgcagcagc cgcgtgatgg tgcgcgccgc gctgtccacg atcgagatgg ctttcgagta
    22261 gcgcattcgc gtcgggccga tcaccccgat gctgccgcgg cggtcgccgt cggtgtagct
    22321 ggccgtcacc aggctgaagg gccggaggtc cggcgcccgg tgctcctccc cgatgatcac
    22381 cgtcaggccg ggcccttcca tgtactcggc caggatgcgc agcaggcgat gcttctcctc
    22441 gatcatcttc agcagcgcgc gcagcgtgcc gagcgagacc gacccctcgg cggtttcctc
    22501 gaccaggccc gcaaacccct cgatgaacag cgcctgctgg gccgacagct cgtcgaaagt
    22561 ggactgcgcc agccgcaggg cgcgcgacat caggcggtcg tagagcgtcc gttcctcggc
    22621 cagccgtgcc agcaccgact cacgcacctt ctgcagcggc cggcccgcga actcctcgtt
    22681 gaggtagttg gccgccatgt gcaggtcgtc ggccgcgatc cgctcgtcga tgtcgaccac
    22741 cttgttggtg atctggccgc ggcgcatgac gacgacgacc aggatgcggg tgtcgcccag
    22801 cggcacgaac tcgatgcgct ggaacgcgtc ttcctcggct gccggggcga gggcgaaccc
    22861 gacgtggtgc gacgcctcgg aaagcacgtg cgaggcggtg gtcaacgcct cttccatcag
    22921 cggcgccccg ccagtctgcg ccagcagctg ggcctcgatc cctggcaccc tcgacggccg
    22981 ccgcgactcg agcagcatgt ccacgtagca gcggtagccg aggtcggtcg gcacccgccc
    23041 cgaagaggtg tgcggctggt agacgtaccc ctgctcctcg agcgaggcca gcacgtttcg
    23101 gaccgtcgcc gaggacaggc cgagcccccc cttgaggacg agcgttcccg acgccaccgg
    23161 cacgccggtt tcgacgtact cggcgaccag ggccgcgaga atgcgacgcg acctgtcgga
    23221 aagcctcgag gtgtacacta gcagtccgtc tcagagagtg ctaagcgcca ttatacacga
    23281 tcggcagggg tccttggccc taaccgccgc tcgctcaacg agtaacgcgg gaaaggctcg
    23341 agcggagcga gcacctggcg acgtgcgccg cggtgaggcg ccccgaccgt gccgccacca
    23401 gctcggcgac gatgctcacg gcgatctcgg ccggggtcac ggcgccgatg tcgaggccga
    23461 tgggggcgtg gagggcttcc agccgctcac gcggcacccc ctcctgggca agggcgtcca
    23521 tcaccttcag gaccttcgcc cggctgccga tgaggcccag gtagatccat gggcgcccga
    23581 tcagggcccg aaccgtttcg agatcctgcc ggtggccgcg ggtgacgacc gcgaagtacc
    23641 cgtcgggtgg aagctcctgc gcctcgaacc aggccgggat ctcgtccacg acgacctcgg
    23701 cggcggggaa gcgctcgcgg ttggcgaacc gctcccgatc gtccaccacg gtcactgcga
    23761 agtcgacgct ggaggcgagg cgggccagtt cgaccgagac gtgccctgcg cccgcgatgt
    23821 acagccgggc cggctggcgg atcggctcca cgaagacctc catctgtccg ccgcagatca
    23881 ggccggtgtc ggcggccagc tcgtcggcca ggctgtagtg cagcagttcg gggcgcccgg
    23941 ttgccagcgc ctcgcgcgcc ttctgggcgg cgtcgtgctc gtagcagccg ccgccgatcg
    24001 tgccgagcgt ccggccgtcg cggaagacga tcattttggc gcccacgcgc tgcggcgtgg
    24061 acccgctggt cgccaccacg gtgacgagcg ccgccgcctc gccccgcgcg gcggcctcga
    24121 cgagcgcctt gaagacgtct actgccatct gaccagcccc cagctcgtag cccccagccc
    24181 ctagccccca gcccccag
//
LOCUS       DAOPQX010000012        23991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2643_length_23991_cov_4.921541, whole genome shotgun sequence.
ACCESSION   DAOPQX010000012 DAOPQX010000000
VERSION     DAOPQX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 23991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23991
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2643_length_23991_cov_4.921541"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKK95_01405"
     CDS             <1..307
                     /locus_tag="PKK95_01405"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOC16889.1"
                     /translation="TEGEIAYCSARRHAAQHFAARFAVKEAAMKALGTGRSRGVLWRD
                     VEVVRRGGPPQLRLHGGAARRFEAMGGRDSLVTITHTDTLAFAQVLLFGHDSHGGAE"
     gene            304..1083
                     /gene="pgeF"
                     /locus_tag="PKK95_01410"
     CDS             304..1083
                     /gene="pgeF"
                     /locus_tag="PKK95_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOC16890.1"
                     /translation="MTGVSDGFHWKDEPWGRSLRSATLAGLADHLFTTCQLRLRGGRE
                     AEEWAAVGRALGVDGGRVARLKQVHGREVVVLKGGAAFLPDAASRPHADIVATDDPSL
                     AIAVQVADCVPLLMAGPGGRVVAAVHAGWRGTCAAAAVGGVEALEREFGARPADLVVA
                     VGPSIGPCCYIVGGEVLEQFEAAHENAARWFRRDGDGRLRLDLWAANADQLEDAGVPR
                     ASIRVSRLCTACHPGLFHSYRRDGPGTGRIAAAIRARGCGP"
     gene            complement(1087..1500)
                     /locus_tag="PKK95_01415"
     CDS             complement(1087..1500)
                     /locus_tag="PKK95_01415"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HOC16891.1"
                     /translation="MVAYIDGGSRGNPGPAGYGVRIEDASGNLVEEFHGSLGVATNNV
                     AEYHGLLAALRWALDHGAGPLLIRSDSELLVKQMLGVYRVRNPGLQPLHREACALARA
                     IGRVRFEHVRRERNTEADRLANEAMDEAAGKATRR"
     gene            complement(1500..2240)
                     /locus_tag="PKK95_01420"
     CDS             complement(1500..2240)
                     /locus_tag="PKK95_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOC16892.1"
                     /translation="MDPDLERLIRLQQIDTFTDAARRRVADHPNLIAALDSRLESAQA
                     GLAAAKEAADQGQVARRSIEKDLAAVQSRLSKYKDQLMEVKTNKEYQAMQKEIEVAQK
                     EVGRLEDRLLEQMLANDDLAAAVKAADARLKDDQKAVAEERARLEKETATLEEELRRK
                     AAARGEVAGQLPADLLASYETIARNRRGQALAEARAGLCTACHVRLRPQVFNEILANN
                     AIRQCDSCQRFLYFAGDAQPDPGAGAAR"
     gene            complement(2250..2325)
                     /locus_tag="PKK95_01425"
     tRNA            complement(2250..2325)
                     /locus_tag="PKK95_01425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2290..2292),aa:Ile,seq:cat)
     gene            2477..3748
                     /locus_tag="PKK95_01430"
     CDS             2477..3748
                     /locus_tag="PKK95_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC16893.1"
                     /translation="MAESDPRTTSLIRLRWGAVAGEAFSLLVARYTFGADLPLSPALG
                     LVGLMAATNVALALAVRGRPLHAPECAAVLAFDVLQLTALLALVGGASNPFSILYLVQ
                     ISMAALILGPRWTWGLAGLAAGAYAALFVLVPAPVMMHDHTGEGFASHLRVMWIAFTA
                     AAALMTYFVARLSGELERRESELRAVRERAVRHERVAALTTLAAGAAHELGTPLGTVA
                     VAAGELHRAVEKVGGPLASAMRDDLRLIRQELDRCRRILDGMAAGSGELVGEAPAAIE
                     ASALVEDVKAALPPGRASRLDARETCGPDRLVAPRRALAGAVANLVQNAFDASPDGRV
                     ELSVAPSPGGGVRITVEDRGPGMAPEVLARVGEPFYTTKPPGQGLGLGLFLARSLADR
                     LGGRFDIESTPGAGTRVSMELPPAATWSARG"
     gene            3745..4296
                     /locus_tag="PKK95_01435"
     CDS             3745..4296
                     /locus_tag="PKK95_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC16894.1"
                     /translation="MSDADFPRSLLIVEDDEALRDRLARAFRDRGYEVRQAADAEAAL
                     AAARDDSPEFVVADLRMPGRSGVEVIRELVALDPSTTAVVLTGYGSIATALEAIRAGA
                     VDYLTKPADVDQIEAALHRRLGPEDRSPDEPGHVPSLARVEWEHINRVLADCGGNLSK
                     AARLLGMHRRSLQRKLSKYPASR"
     gene            4401..6707
                     /locus_tag="PKK95_01440"
     CDS             4401..6707
                     /locus_tag="PKK95_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC16895.1"
                     /translation="MKHSVRLLSALALCSLLLAAGAARAQPPAATVSGTVFGPDGALL
                     PGVSIDVRGPGAKVVRVVSADHGGYRVTGLAPGAYELVTHLPGFVGGAARVEVRAGQI
                     LTVNLSMRLAALSEEVQVIGTAPRDSVEAPRLGESSARDVGEALSGMAGVWKVRRAGV
                     ASDVMVRGYQGENLNVLIDGARLYGACPNNMDHTSFHVDFAEVERVDIAKGPFDMRSQ
                     GGLGGTVSIVTRRPGAGFHATPRLAVGSFGYVNPSATVSFGRERVAVLGGYSYRASDP
                     YTTGAGKPFTEGAGYKNTIDGRRSFEVGTAWGRLDFSPTPAHGLQVSYTWQDADLILY
                     PYLQMDGIWDKAHRANLGYDRRKALGVVDALRGQVYYTKVRHWMTDEFRASSQNMPRA
                     YSMATMARTETVGGRAEATLSRVVAGVEVFRREWETTTSLRMTQYQPQHSIPDVGLTS
                     AGAFVEWSQPLFASLTLDAGGRLDRTRNEADGAKAATALYRAYHDTARTSVSETNPAG
                     KVRLTWSPAPEVTFSGGVGRTVRVPDPQERFFGLRRMGSDWVGNPSLASTTNTGIDVQ
                     VSYRRTGLFLTGSLYRDALDNFIAIYRQARLPGAQGTMNPAARSWRNVNATMTGGEVE
                     GLLSLTDRLFLTGQVSAVRGRQEARAGAAISPNIPEMPPARFRAGLRYDERRAAGGLF
                     GEAEVVYSMEQDRVDASLLEQPTPSWTSVNLRAGFAFKSARVSVGVANLLNRAYAEHL
                     SYQRDPFRSGAKVLEPGRNVYVNLSAVF"
     gene            complement(6845..8527)
                     /gene="rpoD"
                     /locus_tag="PKK95_01445"
     CDS             complement(6845..8527)
                     /gene="rpoD"
                     /locus_tag="PKK95_01445"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOC16896.1"
                     /translation="MSLEDKYDEVRQLISIGKEKGYLLYDEVNDLLRSDITSPDELDD
                     LFNTFGTAGIEVVDSEQKYREDKQEAGEELELDLTPGALDKTNDPVRMYLREMGTVPL
                     LTREGEVEIAKRIERGKLAVIKSISRTPTVARKVIEMGDQLKAGERTIRELVIFNDEE
                     ITDERIADRTREVLRQIDAVRKARQAYEKAVEKLDATAKKDKKKYRRARWKAMRARVN
                     LSQLIRKIEFTEQIKRRLIEDIKEGVERVQRLQREADNLDRLLTAKTRRSRLKEEERK
                     AMAQRLKELRAEVKRQAEVLEETPAQLKRTLDTIRRGEAQAEQAKRELVEANLRLVVS
                     IAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQA
                     RTIRIPVHMIETINKLIRTSRSLVQELGREPTSEEIAKRMDIPVSKVRKVLKIAQEPI
                     SLETPIGEEEDSHLGDFIEDRGIVSPADAVINLNLREQTEAVLKTLTPREEKVIKMRF
                     GVGDGSEHTLEEVGQNFAVTRERIRQIEAKALRKLRHPSRSRKLRAFLEGRT"
     gene            complement(8573..10327)
                     /gene="dnaG"
                     /locus_tag="PKK95_01450"
     CDS             complement(8573..10327)
                     /gene="dnaG"
                     /locus_tag="PKK95_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOC16897.1"
                     /translation="MALFPASFIDDLRARADIVRVVQDYVPLKKAGATWKGLCPFHQE
                     KTPSFQVNPDKGFFYCFGCQTGGDVFKFVELQEKVGFQEAVRLLAAKFGMTAPEPAGA
                     GDRDDAIERETLLKVHERAAAWFREQLAGRAGARARQQLVDRGVSRETADHLGLGFAP
                     PAREALKSWLLRQGFELPLLLRSGLVAQREDGQTVDRFRGRLMIPIARDSGSIVAFGG
                     RSMDPDQQPKYLNSPETPIYSKGRTLYGLNLTKADTRRLGYAVLVEGYFDFAQALQAG
                     VLPVVASCGTALTAQQAHLLRRFASKVVLSYDPDAAGQGAAERSSLLLVQEGFLVNVA
                     LLPPGDDPDGYIRKQGVGPYVDLIRGSRSYLEYLIDRASQGRDFGSDEARREFLNRML
                     PVAAQIPDAAARDQFADRLAHRARITEEVVRAEIRRAAVERRPAVTARELPSFGQVRT
                     AERGLLWGLFQDPETGVEALAALEDGDLEGLVTRRILELARGLHDESPHEVPSALLGR
                     LTMEEARLVTGIAAEPAAPAPLAECVATLKRLRYERERAGLQREIDGLQGGDAEGRMA
                     ELYRRKMELIRLIEQLST"
     gene            complement(10346..12862)
                     /locus_tag="PKK95_01455"
     CDS             complement(10346..12862)
                     /locus_tag="PKK95_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOC16898.1"
                     /translation="MQRAAQQALEFPRIVEAVCVQAATPLGRERLAGLRPHTDPRRVA
                     QALAATTEGTRYLAAGGSFPLQAPPDIQGTLAALAVEGRPLEPARLLGLADFLDSIEQ
                     TRAAIRGAAGPFPLLGSLVEQTASFKAETAEIREKIDASGEVADNASPELRSIRDRLR
                     RQRARLRSTLESYLRGRDTSRYLQDQIVTDRNGRYVLVVRAEHRAAIPGIIHGSSSSG
                     ASLYLEPLSTVDVNNEIVALEEQEAAEVRRILLALTDAFRRRALDLQRTLDAATELDV
                     VQAKARFSSLVGGIEPAISSDGGFELRSARHPLLIRAVAEQLASPESENGEAEEGERR
                     LRRDPREMPAEPVPVDILLAPPATVLVITGPNTGGKTVALKTAGLLALMAQAGLHVPA
                     APGSRLPVFRGVFADIGDEQSIDANLSTFSWHITNIVSMDRMLALPALVLFDELGSGT
                     DPAEGGALAVALVDHFRARGAIVVCTSHAEPLKTYATTTPGVSVAAFGFDPATFEPTY
                     RLVYGSPGRSLALEIAGRLGLRQEVLTAARENLSARDAKLAEHLARIDQNLHDLDHER
                     RLAARERQVLSDAEARVKAREDSLRQREETFRRRVETRLDEQLREARREIDRVVEDLK
                     KKAAELAGAAERRAAKRSGPGISTGDAGAARVEARRALDEAVARFRAGEPERVAPPPP
                     DEHPAEIGDRVVVTGLGLQGTLAAIHGDEAEVEVLGKRVRARTSDLRVASRARGAGLA
                     PAARVSVNVQAAPRADGLTADLNVIGCTVEEALARTERFLDDMLLGDQRTVRVIHGYG
                     TGQLRRAIAEYLRTHPLVSRYDPAPAEQGGGGVTVVELKD"
     gene            complement(12948..13706)
                     /gene="larB"
                     /locus_tag="PKK95_01460"
     CDS             complement(12948..13706)
                     /gene="larB"
                     /locus_tag="PKK95_01460"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOC16899.1"
                     /translation="MTLPPLRDLLDEVAAGRISATDAEQRLHAYLRDLPFEDLGFARV
                     DHHRALRQGFPEVILGTGKSPAQIAAIAGRIVGRGQSLLVTRTTEEAFAAVREQVAGA
                     VFHPEARAITLQQKDIAPGKGPILVAAAGTSDLGVAEEAAVTAELMGNQVDRLYDVGV
                     AGLHRLLRAQNRLTAARVIIVVAGMEGALPSVVAGLVSVPVIAVPTSIGYGASFNGVA
                     ALLGMLNSCAGGVTVVNIDNGFGAATTASLINHL"
     gene            13802..15295
                     /locus_tag="PKK95_01465"
     CDS             13802..15295
                     /locus_tag="PKK95_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC16900.1"
                     /translation="MTPVETLILGAYFFILSILAVYGWHRYYLVYLYMKHKGREVARP
                     EVPAEWPEVTVQLPLFNEMYVVDRLLQAVCRMDYPRERLEIQVLDDSTDETQEIARLA
                     VQRYAALGFDIKYLHRDDRTGFKAGALDAGLRVARGRFVAIFDADFVPRPDFLKKTVP
                     YFAQPRVAMVQARWTHLNGDYSLLTRVQSILLDAHFVLEHGGRNRSGRFFNFNGTAGI
                     WRREAIAAGGGWQHDTLTEDLDLSYRTQLEGWQFVFLPEVEAPAEVPVEMNAFKSQQH
                     RWAKGSIQTCLKLLPRILQSDAPVPVKAEAFFHLTANFNYLLMVVLSVLIFPSMIIRY
                     NMGWYEMLLVDVPLFFAATASIANFYIVSQRELYPDWKTRLKYLPFLMSIGIGLAVNN
                     TRAVVEALLDKQSEFARTPKYGVVRRDSDDWTTKKYRQSVAVQPIIEVALGLYFTWTV
                     FYALANQIYATVPFLMLFQIGFLYTGVLSVVQQFASQGVVLKTEVAK"
     gene            15387..17195
                     /gene="lepA"
                     /locus_tag="PKK95_01470"
     CDS             15387..17195
                     /gene="lepA"
                     /locus_tag="PKK95_01470"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOC16901.1"
                     /translation="MDPLYIRNFSVVAHIDHGKSTLADRFLELTGAVQSREMEAQLLD
                     SMDLERERGITIKAHPVRLTYRADDGQTYLLNLIDTPGHVDFSYEVTRSLAACEGALL
                     LVDAAQGVEAQTLANAYLAVDNNLEVIPVINKIDLPGAQVDETRRQIEEIIGLDGSHA
                     IPTSAKVGTGVRDVLEAVVHRLPPPRGDADAPLKALIFDSWYDPYRGVVIVVRVIDGK
                     LRGRMKVRFMAAGQEYEIEEVGVFTPKATPIDELGVGEVGFITAGIKRVADAKIGDTI
                     TEAARPAAEAFPGFTELKPMVFAGLYPVEGHQYPELRDALEKLRLNDASFFYEPETSA
                     ALGFGFRCGFLGLLHMEIVQERLEREFDQELVTTAPGVLYRVTTTDGEVHEIDSPAKL
                     PEPGRIAVIEEPVITAMILTPPEHVGGILTLCQEKRGVQKALEYLASNRVLITYELPF
                     NEVVLDFYDRLKSISRGYASLDYHVTGHWQSPLVRLDILVNGEPVDALSVIVHRDSAY
                     ARGRSLATRMRQLIPRQMFEVAIQAAVGSRIIARETVKALRKNVLAKCYGGDITRKRK
                     LLEKQKEGKRRMKRVGRVEIPQEAFLAVLRVGDEEQ"
     gene            17197..17955
                     /gene="lepB"
                     /locus_tag="PKK95_01475"
     CDS             17197..17955
                     /gene="lepB"
                     /locus_tag="PKK95_01475"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOC16902.1"
                     /translation="MKHQKAKTAAPAAPAPPAPAAPRRKSTVREYFEALATAVILALF
                     VRTFVVQAFKIPTGSMENNLLIGDHLLVNKMVLAPSPAAVDRLLLPVRDVRRGDVVVF
                     KYPEDPERDFIKRVIGLPGETLEVRDKRVYVDGRPLDEPYVHYLVPPSGSAQFNEVTS
                     YDLRERYGPVTVPTGQYFVMGDNRDNSQDSRYWGFLPREYIKGRALMIYWSYESRSED
                     YVENGLAASLRRLVNVATHFVTRTRWSRIFDPVR"
     gene            18079..18480
                     /locus_tag="PKK95_01480"
     CDS             18079..18480
                     /locus_tag="PKK95_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16903.1"
                     /translation="MVGKLVKIAIALLLLNGIVRLGAAYLADYRFEDRLTQIAQFGDR
                     RSESQLCAEAIEAARQFEVPVAPEDITIRRGTNPPFSCGKGYKGAVSVSRGGAEKLYI
                     DASYQREVALLPGYARPFTFNPSVEVWARVY"
     gene            complement(18505..19764)
                     /gene="hutI"
                     /locus_tag="PKK95_01485"
     CDS             complement(18505..19764)
                     /gene="hutI"
                     /locus_tag="PKK95_01485"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOC16904.1"
                     /translation="MNADFILRHAGRLLTCAGPAPRRGPAQNEVSPIADGAVASLDGR
                     IVFAGTTAACDAEVVPAPGATALDAGGRTVVPGFVDPHTHVVYAGDRREELRRRLAGA
                     AYTEIAAEGGGILSTVRATRQASEEELVSAALPRLAEMLACGTTTAEAKSGYGLETAA
                     ELRMLRAIRTLAARQPIDLAPTFLGAHEIPLEYRERRQQYVDLLVREMIPAVAAGRLA
                     EWCDVFCETGVFTPVESREILRAGQEAGLRARIHADEMAASGGSDVAADVGARSADHL
                     LFVEPGSAEAMARAGVVATLLPGAAFYLKMGRFAPARMLIEHGVAVALATDINPGGGF
                     TPSMPFVMALACFGMRLTLEEALIAATLNAAYAIDRHDRVGSLEVGKSMDAVVVNGEA
                     VDLLRVGAPSIAAVIKNGRVVSGFLPA"
     gene            complement(19773..20363)
                     /locus_tag="PKK95_01490"
     CDS             complement(19773..20363)
                     /locus_tag="PKK95_01490"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOC16905.1"
                     /translation="MKVTPLDLRQQKLTVVFRGYDRNEVDALLNEVADDYERALRETD
                     RLHQELARMESLIAEHREHERNLRNTLLTAQRLADEIREHAEQDAKRTIEEAESRAEL
                     VLQQSQGRIDDLQREIDGLKLKRRDVEASLQSTITALRNALDFVREQDQKEREDKILL
                     HRPRQVAEPAPQTPAASPIMRALDAAAEVQKAQGQS"
     gene            complement(20416..21132)
                     /locus_tag="PKK95_01495"
     CDS             complement(20416..21132)
                     /locus_tag="PKK95_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOC16906.1"
                     /translation="MSLEADLAARLDRVRGRIARAAKASGRDPSDVLLVAVSKTYPAS
                     HVRAAYAAGQRDFGENRVQEALPKIEATSDLPVRWHLVGHLQSNKVRKAATSFPVVHS
                     IDSAPLLEALDRAALAAGVRPEVLVQVDLAGEATKFGVPPENLGQVLEAARSCRAARP
                     AGLMLMPPFFEDAEAARPYFRRLRALRDELLASGRLPASAVHLSMGMSHDLEPAVEEG
                     ATIVRVGTAIFGPRGLTSNA"
     gene            complement(21227..22201)
                     /locus_tag="PKK95_01500"
     CDS             complement(21227..22201)
                     /locus_tag="PKK95_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC16907.1"
                     /translation="MLPVLSGRRALLVVLAVLAAVAIASPGWAQSTGMLKGKVVDGEN
                     KPVDGAKVSISFLDGISRSYEVKTNKKGEFIQIGLPPGRYKVTAEKEKLGSQSFDARV
                     RLGDAAEVNFALVPGSVGLTKEDAAKAEAVKTLFEEGVAASRSNDYDTALAKFNEAIT
                     LLPSCYDCYYNIGFAQAQKKEYDKAEESFKKAIELKADYVEAYNGLATVYNAQKRFDD
                     AQAASQKAAELASAGGATGGGGGVDALYNQGVIAWNAGKAEDAKKHFEEAIKLNPQHA
                     DSHYQLGMCYVNLGNLAEAAGMFEKYLTLAPEGQYAEQAKALVTQLKK"
     gene            complement(22319..>23991)
                     /locus_tag="PKK95_01505"
     CDS             complement(22319..>23991)
                     /locus_tag="PKK95_01505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOC16908.1"
                     /translation="DLRRALGIDFTATCLKGRGNYLCLHRFDQFRENPLSLALVEPEV
                     ADLLASWGRQTDTGDRAELEGLPDDLEVWAELSASSENCIGPSCPRHEDCFVTRMRRR
                     AEQSDLVVVNHHLLCADASVRQESAGGVIPECEVAVIDEAHQLEEVATQYFGVAVSTY
                     RIEDLLHDATRALVAAASLDEAARGGIRRGLEEVADRAPRFFDALRRARGNLRGEERA
                     RVAPETLAEVQQPAASLCEALDEVQGEIALAPSPPEDLRAIARRAGEVHDHLAFLVRA
                     GDPDFVYYLETRGRGIALRASPIDVSAIVRELLLDRLPATVLTSATLAVGGSFGYVRG
                     RLGVGDADEIRVPSEFAYREQAILYLPPRMPDPRSPQFPAAATREILQILERTEGRAF
                     VLFTSYAMLREVERSLPPSLPFPVLVQGQAPRSVLLRRFRSLGNAVLLATSSFWQGVD
                     VVGEALSCVIVDKIPFASPADPVTAARIEAIQARGESPFTEYQVPLAVLTLLQGFGRL
                     IRHRQDRGVLALLDPRVRTKGYGAVFLDALPPAPVTSRLDDLTRFFAR"
BASE COUNT         3389 a         8446 c         8416 g         3740 t
ORIGIN      
        1 caccgagggc gaaatcgcct actgcagcgc acgccgccac gccgcccagc acttcgcggc
       61 acgcttcgca gtcaaggagg cggccatgaa ggcgctcggc accggccgct cgcggggtgt
      121 cctctggcgc gacgtcgagg tcgtccggcg cggcggaccg ccgcaactgc ggttgcacgg
      181 cggcgcggcg cggcgcttcg aggcgatggg tggacgcgac tcgctggtga ccatcaccca
      241 caccgacacg ctcgccttcg cgcaggtgct tctcttcggc cacgactcgc acgggggcgc
      301 ggagtgaccg gcgtatcgga cggcttccac tggaaggacg agccgtgggg gcgatcgctg
      361 cgctcggcga cgctggccgg cctggcggac cacctcttca ccacctgcca actgcggttg
      421 cggggagggc gcgaggcgga ggagtgggcc gcggtcggcc gggccctggg agtggacggc
      481 gggcgcgtcg cgaggctgaa acaggtgcac ggccgcgagg ttgtggtcct gaaggggggc
      541 gccgcgttcc tcccggatgc cgcctcccgg ccccacgccg acatcgtcgc caccgacgac
      601 ccgtcactgg ccatcgccgt gcaggtggcc gactgcgtgc cgctgctgat ggcggggccc
      661 ggcgggcggg tggtggcggc ggttcacgcc ggctggcgcg gcacgtgtgc ggccgccgcg
      721 gttggcggcg tcgaggcgct cgagagggag ttcggcgcaa ggccggccga cctggtcgtg
      781 gcagtggggc ccagcatcgg gccgtgctgc tacatcgtcg ggggcgaggt gctcgagcag
      841 ttcgaggcag cgcacgagaa cgcggcccgc tggttccgcc gcgacggtga tggacggctg
      901 cgcctcgatc tgtgggccgc caacgccgac caactggagg acgccggcgt gccccgcgct
      961 tccattcgag tgtctcgcct ctgcaccgcg tgccatcccg ggctgttcca ctcctatcgc
     1021 cgtgacggcc ccggcacggg gaggattgcc gcggcgatca gggcgagggg atgcggcccg
     1081 taaggcttac cgccgggtcg ctttgcctgc cgcctcgtcc atcgcctcgt tggcgaggcg
     1141 gtcggcctcg gtgttgcgct cgcggcggac gtgctcgaag cggacccggc cgatggcgcg
     1201 tgccagggcg cacgcctcgc ggtgaagtgg ctggaggccg gggttcctga cgcgatagac
     1261 gccgagcatc tgcttgacga gcagctcgga gtcggagcga atcagaagcg ggccggcgcc
     1321 gtggtcgagc gcccagcgga gggcggcgag cagcccgtga tactcggcga cgttgttggt
     1381 ggccacgccg agcgacccgt ggaactcctc gacgaggttg cccgacgcat cctcgatgcg
     1441 gacgccgtac ccggcggggc ctgggttgcc gcgggagccg ccgtcgatgt acgcgaccat
     1501 caccgggccg ctccggcccc gggatcaggc tgcgcgtcgc cggcgaagta aaggaagcgc
     1561 tggcagctgt cgcactggcg gatggcgttg ttggcgagga tctcgttgaa gacctgcggc
     1621 cgaagcctga cgtggcaggc cgtgcagagc ccggcgcggg cctcggcgag cgcctggccc
     1681 cgccggttgc gggcgatcgt ctcgtaactg gcgagcaggt cggcgggaag ctggccggca
     1741 acctcgccgc gggcggcggc cttgcggcgc agctcctcct cgagcgtcgc ggtttccttc
     1801 tcgaggcggg cgcgctcttc ggcgaccgcc ttctggtcgt ccttcaggcg ggcgtcggcg
     1861 gccttgacgg cggcggccag gtcgtcgttg gcgagcatct gttcgaggag gcggtcctcg
     1921 aggcggccga cttccttctg cgccacctcg atctccttct gcatcgcctg gtattccttg
     1981 ttggtcttca cctccatcaa ctggtctttg tatttcgaga ggcgcgactg gacggcggcg
     2041 agatccttct cgatggaccg gcgcgcaacc tgtccctggt cggcggcctc cttggccgcg
     2101 gccaggccgg cctgcgccga ctcgaggcgg ctgtcgagcg cggcgatcag gttcggatga
     2161 tccgcgaccc gccgccgggc ggcgtctgtg aaggtgtcga tttgctgcag gcggatgagg
     2221 cgttcgagat caggatccat ggaaatctgt ggtgggccca ccaggattcg aacctgggac
     2281 aggccggtta tgagccggcg gctctgaccg ctgagctatg ggcccgggat ccgacgcgac
     2341 gagcgggccg gcggggcagc cggcgtgacc agtctactcc tgtccgttct ccaccgggca
     2401 ttttacctct ccgggcgtga accccgcggg agcaggtgtg ctatcgtgag gcccggcgag
     2461 ccggaaggtg cgcacggtgg cagaatccga cccacgcacg acttccctga tccgcctgcg
     2521 gtggggcgcc gtggccggcg aggcgttcag cctcctggtc gcgcggtaca ctttcggcgc
     2581 cgaccttccc ctgtcacccg ccctcgggct ggtgggcctg atggcggcga ccaacgtcgc
     2641 tctcgcgctg gcggtgcgcg gccgtccgct ccacgcgccc gagtgcgccg cggtgctcgc
     2701 gttcgacgtc ctgcagctca ccgcgctgct cgcgctggtc ggcggagcct cgaacccgtt
     2761 cagcatcctg tacctcgtcc agatcagcat ggcggcgctc atcctcggcc cgcgctggac
     2821 gtggggcctg gcggggcttg cggccggcgc ctacgcggcg ctgttcgtgc tggtgcctgc
     2881 gccggtgatg atgcacgacc acaccggaga aggcttcgcc tcacacctgc gcgtgatgtg
     2941 gatcgcgttc acggcggcgg cggccctgat gacgtacttc gtggcgcgcc tgtcgggcga
     3001 gctcgaacgc cgcgagtcgg agctgcgggc ggtgcgcgag cgagccgtcc ggcacgagag
     3061 ggtggccgcc ttgacgacgt tggccgcggg ggcggcacac gagctgggga cgccgctcgg
     3121 aaccgtggca gtggcggccg gcgaactgca tcgggcggtc gagaaggtgg ggggaccact
     3181 ggcctcggcg atgcgggacg acctgcgcct gatccggcag gagctcgatc gctgccggcg
     3241 catcctcgat gggatggcgg ccggctcggg cgaactggtg ggcgaggcac cggcagccat
     3301 cgaagcgtcg gcgctggtcg aggacgtgaa ggcggcgctg ccgcccggcc gcgcgtcgcg
     3361 cctcgatgcc cgggagacgt gcggccccga ccggctcgtc gcgcctcgcc gggcgctcgc
     3421 cggcgcggtg gcgaacctgg ttcaaaacgc cttcgacgcc tcgcctgacg gccgcgtcga
     3481 gctgagtgtc gcaccgtcgc ccggcggtgg cgtgcggatc accgtcgagg accgcggtcc
     3541 cggcatggcc cccgaggtgc tggcgcgcgt gggcgagccg ttctacacga cgaagccgcc
     3601 cgggcagggt cttggcctcg ggctgttcct ggcccggtcg ctggccgacc ggctgggcgg
     3661 ccggttcgac atcgaatcca cgccgggtgc cggcacccgc gtgtcgatgg agcttcctcc
     3721 ggcggcgacc tggagcgcac gagggtgagc gacgccgact ttccacgttc actgctgatc
     3781 gtcgaggacg acgaggcgct gcgcgaccgg ctggcgcgcg cgttccgcga tcgcgggtac
     3841 gaggtgcggc aggcggccga cgccgaggcg gcgctcgctg cggcccgaga cgactcaccc
     3901 gagttcgtcg ttgccgacct caggatgcct ggccgctcgg gtgtggaggt gatccgcgag
     3961 ctggtcgccc tcgatccctc gaccacggcc gtcgtgctca ccgggtacgg atcgatcgcg
     4021 accgcgctcg aggcgatccg tgccggtgcc gtggactacc tgaccaagcc ggccgacgtg
     4081 gaccagatcg aggccgcgct gcaccgccgc cttggccccg aggatcgttc ccccgacgag
     4141 ccgggccacg tgccgtcgct cgcgcgcgtc gagtgggagc acatcaatcg cgtgctcgcc
     4201 gattgcggcg gcaacctctc gaaggcggcg cgcctgctgg gcatgcaccg ccgctcgctg
     4261 cagcgcaagc tctccaagta ccccgccagc cgctgacgca acctccgcgc aggactttcg
     4321 taccaagacc tcggtcctgc gacaacaaac cgcattccgc cggcaccttc gggtcgtgga
     4381 gactacccgt gagggtactc atgaaacact cggtccgcct cctgtcggct ctcgctctct
     4441 gcagtctcct gctggccgcc ggcgcagccc gcgcgcagcc gcccgccgcg acggtgagcg
     4501 ggacggtctt cggtcccgac ggcgcgctgc ttcccggcgt ttccatcgac gtgcggggcc
     4561 ccggggcgaa ggtcgtccgc gtcgtctcgg ccgatcacgg cggctaccgt gtcacgggcc
     4621 tcgcacccgg cgcgtacgaa ctcgtcacgc atctcccggg cttcgtggga ggcgcggcgc
     4681 gcgtcgaggt gagggccggg cagattctca cggtgaacct gtcgatgcgc ctggcggccc
     4741 tctcggaaga agtgcaggtg attggtacgg cccctcgcga cagcgtcgag gcgccgcggc
     4801 tcggcgagag cagcgcccgg gacgttggcg aggccctgtc ggggatggcg ggcgtgtgga
     4861 aggtcaggag ggccggcgtc gccagcgacg tgatggtgcg cggctaccag ggtgagaacc
     4921 tgaacgtgtt gatcgacggc gccaggctct acggcgcctg cccgaacaac atggaccaca
     4981 cgtcgttcca cgtcgacttc gccgaggtcg agcgggtgga catcgccaag ggacccttcg
     5041 acatgcggag ccagggcggc ttgggcggca cggtgagcat cgtcacgcgg cggcccggtg
     5101 ccggcttcca cgccacgccc cgcctcgcgg tcggctcgtt cgggtacgtg aacccgtcgg
     5161 ccacggtttc gttcgggcgc gagcgggtgg cggtgctcgg cggctactcg taccgcgcgt
     5221 cggacccgta cacgaccggg gccgggaagc ccttcaccga gggcgcgggc tacaagaaca
     5281 ccatcgacgg ccggcggtcg ttcgaggtgg gcaccgcgtg ggggcgcctc gatttctcgc
     5341 ccacgcctgc ccacggcctg caggtgtcct acacgtggca ggacgccgac ctcattctct
     5401 acccctacct tcagatggac gggatctggg acaaggctca ccgggcgaac ctgggctacg
     5461 accgccggaa ggcactcggc gtcgtggacg cgttgcgcgg gcaggtctac tacacgaagg
     5521 tgcggcactg gatgaccgac gagttccgcg cctcgtcgca gaacatgccg cgcgcctaca
     5581 gcatggcgac gatggcgagg accgagaccg tcggggggcg cgccgaggcc acgctcagcc
     5641 gcgtggtggc gggcgtcgag gtgttccgtc gcgagtggga gacgacgacc tcgttgcgga
     5701 tgacgcagta ccagccgcag cactcgattc ccgacgtcgg cctcacctcg gccggggcgt
     5761 tcgtcgaatg gtcgcagccg ctcttcgcct cgctgacgct cgatgccggc ggccggctgg
     5821 atcggacgcg gaacgaggcg gacggggcca aggcggcaac cgccctctac cgcgcgtatc
     5881 acgacacggc gcgaaccagc gtcagcgaga cgaacccggc cggcaaggtg aggctgacgt
     5941 ggtcgccggc gccggaggtg accttcagcg gcggcgtcgg acggacggtg cgcgtgcccg
     6001 acccgcagga gcggttcttc gggctgcggc gcatggggtc ggactgggtg ggcaacccct
     6061 cgcttgcgag cacgacgaac accggcatcg acgtacaggt cagctaccgc aggaccgggc
     6121 tcttcctcac gggctccctc taccgcgatg ccctcgacaa cttcatcgcg atctaccgcc
     6181 aggctcgcct ccccggcgcc cagggcacga tgaaccccgc ggctcgctcc tggcgcaacg
     6241 tgaacgcgac catgacgggc ggcgaggtgg agggactgct gtcgctcacc gaccgcctgt
     6301 tcctgaccgg ccaggtgagt gcagtgcgag ggaggcagga ggcacgcgcg ggagccgcca
     6361 tcagccccaa catcccggag atgccgccgg cccgcttcag ggccgggctg aggtacgacg
     6421 agcgccgcgc cgcgggcggc ctgttcgggg aggccgaggt cgtctactcg atggagcagg
     6481 accgcgtgga tgcctcgctc ctcgagcagc cgacgccgtc gtggacttcg gtgaacctgc
     6541 gggcggggtt cgcgttcaag tcggcgcgcg tgtcagtggg ggtggcgaac ctgctgaacc
     6601 gggcctacgc ggagcacctc tcctaccagc gcgacccgtt ccgatcgggc gcgaaggtgc
     6661 tggagccggg acggaatgtg tatgtgaacc tgtcggcggt gttctagcag cggacagcag
     6721 gcagcggaca gcagacagcg gacagcagac agcgaaaagg cccggcgcgg attgcgcgcc
     6781 gggccttctt ctgcgtgtct gaactcggag ctgtcagctg tcagccgtac gctgaccgct
     6841 gcccctaggt gcgtccctcc aggaacgctc gcagcttgcg gctgcgcgag gggtggcgca
     6901 gcttccgcag cgccttggcc tcgatctgcc ggatgcgctc gcgggtgacc gcgaagttct
     6961 ggcccacctc ctcgagcgtg tgctcgctgc cgtctcccac cccgaagcgc atcttgatca
     7021 ccttctcctc gcgcggcgtg agcgtcttga gcaccgcctc ggtctgctcg cggaggttca
     7081 ggttgatcac ggcatcggcc ggcgagacga tgccgcggtc ctcgatgaag tcgccgaggt
     7141 ggctgtcctc ctcctcgccg atgggagtct ccagtgagat aggctcctgg gcgatcttga
     7201 ggaccttgcg gaccttcgac acggggatgt ccatccgctt ggcgatctcc tcggaggtcg
     7261 gctccctccc cagctcctgc acgagcgacc gcgaggtgcg gatgagcttg ttgatcgtct
     7321 cgatcatgtg gaccgggatg cggatggtcc gggcctggtc ggcgatcgcg cgggtgatgg
     7381 cctggcgaat ccaccaggtc gcgtaggtcg agaacttgta gccgcggcga tactcgaact
     7441 tgtccacggc cttcatgagg ccgatgttcc cctcctggat caggtcgagg aactgcaggc
     7501 cgcggttggt gtacttcttc gcgatcgaga cgacgaggcg gaggttggcc tcgaccagct
     7561 cgcgcttggc ctgctccgcc tgcgcctccc cgcgccggat ggtgtcgagc gtccgcttga
     7621 gctgggcggg cgtctcctcg aggacttcgg cctggcgctt cacctcggcg cgcagctcct
     7681 tcagccgctg cgccatggcc tttcgctcct cctccttcag gcgcgaccgg cgcgtcttgg
     7741 cggtcaggag ccgatcgagg ttgtcggcct cgcgctgcag ccgctgcacg cgctcgaccc
     7801 cctccttgat gtcctcgatc aggcggcgct tgatctgctc ggtgaactcg atcttccgga
     7861 tcagctgcga caggttcacc cgggcgcgca tcgccttcca tcgcgcccgg cggtacttct
     7921 tcttgtcctt cttcgcggtg gcgtcgagct tctcgacggc cttctcgtac gcctgccgtg
     7981 ccttccggac ggcgtcgatc tggcgcagga cctcgcgcgt ccggtcggcg atccgctcgt
     8041 cggtgatctc ctcgtcattg aagatcacca gctcgcggat cgtccgctcg ccggccttca
     8101 actggtcgcc catctcgatg accttgcggg ccaccgtcgg cgtccgcgag atcgacttga
     8161 tcacggcgag cttgccgcgc tcgatccgct tggcgatctc gacctcgccc tcgcgcgtca
     8221 gcagggggac cgtgcccatc tcgcgcaggt acatccggac cgggtcgttc gtcttgtcca
     8281 gggcgccggg cgtgaggtcg agctccagct cctcgccggc ctcctgcttg tcctcccggt
     8341 acttctgctc ggaatcgacg acctcgatgc cggccgtgcc gaacgtgttg aacaggtcgt
     8401 cgagttcgtc gggggaggtg atgtcggacc gcagcaggtc gttcacctcg tcgtagagca
     8461 gatagccttt ctccttgccg atggagatca gctgccggac ctcgtcgtat ttgtcctcga
     8521 gcgacaaggg cgtctcctct caagtctccg cgcgtctgac gtcacgtcag cgctaggtcg
     8581 aaagctgttc gatcagccga atgagttcca tcttccgccg gtacaactcc gccatccgcc
     8641 cctcggcgtc gcccccctgc aggccgtcga tctcgcgctg caggccggcc cgctcgcgct
     8701 cgtaccgcag ccgcttgagc gtcgctacgc attcggcgag gggcgccggc gcggcaggct
     8761 cggccgcgat gccggtcacc agccgcgctt cctccatagt tagacgtccg agaagcgccg
     8821 aggggacctc atgcggcgac tcatcgtgca gtccccgcgc caattcgaga atccgccggg
     8881 tgaccaggcc ctcgaggtcg ccgtcttcga gggccgccaa cgcctcgacg ccggtctccg
     8941 ggtcctggaa gagcccccag agcagcccgc gctcggcggt cctcacctgg ccgaagctcg
     9001 gcaattcccg tgccgtcacg gccggccgcc gctccaccgc cgcccgccga atctcggccc
     9061 gcaccacctc ctcggtgatc cgggcccggt gggccaggcg gtcggcaaac tggtcgcggg
     9121 ccgccgcgtc cgggatctgc gccgcgaccg gcagcatccg gttcaggaac tcgcggcgcg
     9181 cctcgtcgct gccgaagtcg cggccctggg acgcccggtc gatcaagtac tcgaggtaag
     9241 accgcgagcc tcgaatcaga tccacgtacg gcccgacccc ctgcttgcgg atgtagccgt
     9301 cggggtcgtc acccggcgga agcagcgcca cgttcaccag gaacccttcc tgcaccagca
     9361 gcagcgacga ccgctcggcc gccccctgcc cggccgcatc cgggtcgtag ctgagcacca
     9421 ccttcgacgc aaaacgacgc agcaggtgcg cctgctgcgc ggtcaacgcc gtcccgcacg
     9481 aggcgactac cggcagaacc cccgcctgca gcgcctgggc gaagtcgaaa tacccctcca
     9541 ccagcaccgc gtacccgagc cggcgggtgt ctgccttcgt caggttcagc ccgtaaagcg
     9601 tccggccttt cgagtaaatc ggcgtctcgg gcgagttcag gtacttcggc tgctggtcgg
     9661 gatccatgga ccggccgccg aaggccacga tcgagccgct gtcgcgggcg atcgggatca
     9721 tcagccggcc gcggaagcgg tcgaccgtct gcccgtcctc gcgctgcgcc accaggccgc
     9781 tccggagcag cagcggcagc tcgaacccct gcctcaagag ccacgacttc aacgcctcgc
     9841 gtgccggggg ggcgaagccg agccccaggt ggtcggccgt ctcgcggctg accccccggt
     9901 caaccagctg ctgcctggcc ctcgccccgg ccctccccgc cagctgctcg cggaaccacg
     9961 ccgccgcgcg ctcgtgcacc ttgagcagcg tctcgcgttc gatcgcgtcg tcccggtcgc
    10021 cggcgccggc cggttcgggc gccgtcatcc cgaacttcgc ggcgagcagg cggacggcct
    10081 cctggaagcc caccttctcc tgcagctcga cgaacttgaa gacgtcgccc ccggtctggc
    10141 agccaaagca gtagaagaac cccttgtccg ggttcacctg gaaggagggc gtcttctcct
    10201 ggtggaacgg gcacagcccc ttccaggttg cgcccgcctt cttcagcggc acgtagtcct
    10261 gcaccacccg gacgatatct gcccgggcgc gcaggtcgtc gataaaagac gcgggaaaga
    10321 gggccacggt tacagctggc gctcgtcagt ctttcagctc caccaccgtc accccgccgc
    10381 cgccctgctc cgccggcgcc gggtcgtacc gggacacgag cgggtgcgtt cgcaagtact
    10441 ccgcgatcgc tcgccgcagc tggccggtgc catacccgtg aatcacccgc accgtgcgct
    10501 ggtcgccgag cagcatgtcg tcgaggaacc gctcggtgcg cgcgagcgct tcctccacgg
    10561 tgcagccgat gacgttcagg tcggcggtca gcccgtcggc ccgcggcgcc gcctgcacgt
    10621 tcacgctgac acgggccgcc ggggccagcc ccgccccgcg cgccctcgag gccacccgaa
    10681 ggtcggaggt tctcgcccgg acccgcttcc ccagcacctc gacctccgcc tcgtcaccgt
    10741 ggatggccgc cagcgtgccc tgcaggccca gtccggtgac gacgacacgg tcgccgatct
    10801 cggcggggtg ctcgtcggga ggcggcgggg cgacgcgttc cggctcgccg gcgcggaagc
    10861 gcgcgacagc ctcgtcgagg gcccgccggg cctccacccg cgcagcacct gcatcccccg
    10921 tggagatgcc cggcccgctt cgcttcgccg cccggcgttc ggccgccccg gccagctcgg
    10981 cggccttctt cttgaggtcc tcgacgacgc ggtcgatctc gcgccgcgcc tcgcgaagct
    11041 gctcgtcgag ccgcgtttcg acgcgacggc ggaatgtctc ctcgcgctgc cgcaggctgt
    11101 cctcgcgggc cttcacccgg gcctcggcgt cgctcagcac ctgccgctcc cgcgccgcca
    11161 gccggcgctc gtggtcgagg tcgtgcaggt tctggtcgat gcgcgccaag tgctcggcca
    11221 gcttggcgtc acgggcgctc aggttctcgc gcgccgccgt cagcacctcc tggcgcagtc
    11281 ccaaccggcc cgcgatctcg agcgccaggc tgcgcccggg cgagccgtag acgaggcggt
    11341 aggtcggctc gaaggtggcc gggtcgaagc cgaaggccgc gaccgagacc ccgggggtcg
    11401 tggtcgcgta ggtcttcagc ggctccgcgt ggctggtgca gacgacgatg gcgccacgcg
    11461 cccggaagtg atccaccagg gccaccgcca gggcgcctcc ctcggcgggg tcggtgcccg
    11521 acccgagctc gtcgaagagc accagtgccg gcagcgccag catccggtcc atcgacacga
    11581 tgttggtgat gtgccaggag aaggtgctga ggttggcgtc gatcgactgc tcgtcgccga
    11641 tgtcggcgaa cactccccgg aacaccggga gcctcgaccc cggggccgcg gggacgtgca
    11701 ggccagcctg cgccatcagc gccaggaggc cggccgtctt cagcgcaacc gtcttgccgc
    11761 cggtgtttgg cccggtgatg acgaggacgg tggcgggcgg cgccagcagg atgtccaccg
    11821 gcaccggctc ggcgggcatc tcgcgggggt cgcggcgaag gcggcgctcc ccctcctccg
    11881 cctcgccgtt ctcgctctcc gggctggcca actgctcggc gaccgcccga atcagcaggg
    11941 gatggcgggc cgaccgcagc tcgaaaccgc cgtcgctcga gatcgccggc tcgatgccgc
    12001 ccaccaggga cgagaagcgg gccttggcct ggaccacgtc gagctcggtc gcggcgtcca
    12061 gcgtccgctg cagatcgagc gcccggcggc ggaaggcatc ggtgagcgcc agcaagatgc
    12121 gccggacctc cgccgcttcc tgctcctcca gcgccacgat ctcgttgttg acgtccaccg
    12181 tgctgagggg ctcgaggtag aggctcgcgc cgctcgacga gctgccgtga atgatcccgg
    12241 ggatggccgc ccggtgctcg gcgcggacga ccagcacgta gcggccgttg cggtcggtga
    12301 cgatctggtc ctgcaggtag cgggaggtgt cgcgcccccg gaggtacgac tcgagcgtgc
    12361 tgcgcaggcg ggcccgctgg cggcgcaggc ggtcacggat cgatcgcagc tcggggctcg
    12421 cgttgtcggc cacctcgccg ctcgcgtcga tcttctcgcg gatctccgct gtctcggcct
    12481 tgaacgaggc agtctgctcg accaggctcc cgagcagggg gaagggcccg gcggccccgc
    12541 gaatggcagc ccgcgtctgt tcgatggagt cgaggaaatc ggccaggccg agcagccggg
    12601 ccggctcgag cgggcgccct tcgacagcca gcgcggccag ggtgccctgg atgtcgggcg
    12661 gcgcctgcag ggggaacgaa ccgccggcgg cgagatagcg agtgccctct gtcgtcgcag
    12721 cgagcgcctg cgcgacccgg cgcgggtcgg tgtgcgggcg aaggccggcg aggcgctccc
    12781 gcccgagggg cgtggccgcc tggacgcaca ccgcctcgac gattcgcggg aactccaagg
    12841 cctgttgggc ggctcgctgc atagaagggt gggtgacccg gctatccgga ggcaacctcc
    12901 cacactaaca caaccaggcg gcgcgcgcaa cgctcggagc ccggcggcta caggtgattg
    12961 atcaggctcg ccgtcgtcgc ggcgccgaac ccgttgtcga tgttgaccac cgtcaccccg
    13021 ccggcgcagc tgttgagcat cccgagcagg gccgcgaccc cgttgaagct ggccccgtag
    13081 ccgatgctgg tcgggacggc aattaccggc acgctgacca ggccggccac gacgctcggg
    13141 agggctcctt ccatgccagc tacgacgatg atcacgcgtg ccgcagtgag gcggttctgc
    13201 gccctgagga ggcggtgcag acctgcgacg ccgacgtcgt acaaacggtc gacctggttg
    13261 cccatcagct cggccgtgac ggcggcctcc tcggccacgc cgaggtcgga cgtgccggcc
    13321 gcggccacca ggatcggccc cttcccggga gcgatgtcct tttgctgcaa ggtgatagca
    13381 cgagcttcgg ggtggaacac ggcgcccgcc acctgctcgc ggaccgccgc gaaggcctcc
    13441 tccgtcgtgc gggtgaccag caggctctgg ccccggccga cgatgcgccc ggcgatggcg
    13501 gcgatctggg ccggactttt gcctgtacca aggatgacct cgggaaagcc ctggcgcaga
    13561 gcccggtgat gatctacccg ggcaaaaccg aggtcctcga acggcaggtc gcggaggtaa
    13621 gcgtggagcc gctgttcggc atcggtcgcc gagatccggc ccgcggcgac ctcgtcgagg
    13681 agatcgcgga gaggcggaag agtcatcggt caccccggta cgggggggcg ggtggcaatt
    13741 gacgtttcat ggctgggcaa tataatgact tctttgttcc cgtccccgac acggctttcc
    13801 tatgacgccc gttgaaaccc tcatcctcgg ggcgtacttc ttcatcctca gcatcctggc
    13861 ggtatacggc tggcaccgtt actaccttgt gtatctttac atgaagcaca agggacgcga
    13921 ggtggcccgg ccggaggtgc cggccgagtg gcccgaggtc accgtccagc tccccctctt
    13981 caacgagatg tacgtcgtcg accggctgct ccaggcggtc tgccggatgg actacccgcg
    14041 cgagcggctc gagatccagg ttctcgacga ttccaccgac gagacgcagg agatcgcgcg
    14101 gctcgcggtg cagcgctacg ccgccctggg cttcgacatc aagtacctgc accgagacga
    14161 ccggaccggg ttcaaggccg gagcgctcga cgcggggctg cgggtggcgc gcggccgctt
    14221 cgtggcgatc ttcgacgccg acttcgtccc ccggcccgac ttcctcaaga agacggtccc
    14281 gtacttcgcc cagccgcggg tggccatggt ccaggcgcgc tggacccacc tcaacggcga
    14341 ctactcgctg ctgacgcggg tgcagtcgat cctgctcgac gcgcacttcg tgctcgagca
    14401 cggcgggcgg aaccgctcgg ggcggttctt caacttcaac gggaccgccg gcatctggcg
    14461 gcgggaggcg attgccgcgg gcggcgggtg gcagcacgac acgctcaccg aggacctcga
    14521 cctcagctac cgcacgcagc tcgaaggctg gcagttcgtg ttcctccccg aggtcgaggc
    14581 ccctgccgag gtgccggtcg agatgaacgc cttcaagtcg cagcagcacc gctgggccaa
    14641 ggggtcgatc cagacctgcc tcaagctgct gccgcggatc ctgcagtcgg acgcgccggt
    14701 gccggtgaag gccgaggcgt tcttccacct cacggcgaac ttcaactacc tgctgatggt
    14761 cgtgttgtcg gtgctgatct tcccgtcgat gatcatccgc tacaacatgg gctggtacga
    14821 gatgctgctg gtggacgtgc cgctgttctt cgcggccacc gcgtccatcg cgaacttcta
    14881 catcgtctcc cagcgcgagc tctaccccga ctggaagacc cgcctgaagt acctcccgtt
    14941 cctgatgtcg atcggcatcg gcctggcggt gaacaacacc cgcgcggtgg tcgaggcgct
    15001 gctcgacaag cagagcgagt tcgcccggac gccgaagtac ggcgtcgtgc ggcgcgacag
    15061 cgacgactgg acgacgaaga agtaccgtca gtcggtcgcc gtccagccca tcatcgaggt
    15121 tgcgctcggc ctgtacttca cctggaccgt gttctacgcg ctggcgaacc agatctacgc
    15181 cacggtgccg ttcctgatgc tgttccagat cgggttcctc tacaccggcg tgttgtcggt
    15241 cgtgcagcag ttcgccagcc agggcgtcgt cctcaagacc gaggtcgcga agtagtcgcg
    15301 cgggacgggc gtcgctgccc gtcccctctc cctccgcccc ttccgcgctc cgctccgcct
    15361 gactgctata cttatccgat cgcccgatgg acccgctgta catccgcaac ttttcggtcg
    15421 tcgcgcacat cgaccacggc aagagcacgc tggccgaccg tttcctcgag ctgacggggg
    15481 ccgtgcagtc gcgagagatg gaggcccagc tgctcgacag catggacctc gagcgggagc
    15541 gcggcatcac gatcaaggcg cacccggtga ggctcaccta ccgggccgac gacggccaga
    15601 cctacctcct caacctgatc gacacccccg ggcacgtgga cttctcgtac gaggtgaccc
    15661 gctcgctcgc ggcctgcgag ggcgcgctgc tgctggtgga cgcggcgcag ggggtcgagg
    15721 cgcagaccct cgccaacgcc tacctcgcgg tggacaacaa cctcgaagtc atcccggtca
    15781 tcaacaagat cgacctcccg ggcgcccagg tggacgagac gaggcggcag atcgaggaga
    15841 tcatcgggct cgacggcagc cacgcgatcc cgacctcggc gaaggtcggc accggcgtgc
    15901 gcgacgtgct cgaagcggtc gtgcaccggc tgccgccgcc ccgcggcgac gccgacgcgc
    15961 ccctgaaggc gctgatcttc gactcttggt acgaccccta ccgcggcgtg gtcatcgtcg
    16021 tccgcgtcat cgacggcaag ctgcgcgggc ggatgaaggt gcgcttcatg gcggccgggc
    16081 aggagtacga gatcgaggag gtcggcgtgt tcacccccaa ggcgacgccc atcgacgagc
    16141 tgggcgtcgg cgaggtgggg ttcatcacgg ccggcatcaa gcgggttgcc gacgcgaaga
    16201 tcggcgacac catcaccgag gcggcgcgcc cggcggccga ggcgttcccc ggcttcaccg
    16261 agctgaagcc gatggtcttc gccgggctgt acccggtgga aggccaccag taccccgaac
    16321 tgcgcgacgc cctggagaag ctgcgtctca acgacgcctc gttcttctac gagcccgaga
    16381 cgtcggccgc cctcggcttc gggttccggt gcgggttcct gggcttgctc cacatggaaa
    16441 tcgtgcagga gcggctcgag cgggagttcg accaggagtt ggtgacgacc gctcccggcg
    16501 tgctctacag ggtcacgacc accgacggcg aggtccacga gatcgacagc cccgccaagc
    16561 tgcccgagcc gggccgcatc gcggtcatcg aggagccggt catcacggcg atgatcctca
    16621 cgcctcccga gcacgtcggg gggatcctca cgctctgcca ggagaagcgg ggcgtgcaga
    16681 aggcgctcga gtaccttgcc tccaaccggg tgctgatcac ctacgagctg ccgttcaacg
    16741 aggtcgtgct cgacttctac gaccgcctca agtcgatctc gcgcgggtac gcctcgctcg
    16801 actatcacgt gaccggccac tggcagtcgc cgctggtgag actcgacatc ctggtgaacg
    16861 gtgagccggt ggacgccctg tcggtgatcg tccaccgcga ctcggcgtac gcccgcgggc
    16921 ggtcgctcgc caccaggatg cggcagctga ttccccggca gatgttcgag gtggccatcc
    16981 aggcggcggt gggcagccgg atcatcgccc gcgagaccgt gaaggcgctc cggaagaacg
    17041 tgctcgcgaa gtgctacggc ggcgacatca cgcgcaagcg caagctcctc gagaagcaga
    17101 aggaggggaa gcgccgcatg aagcgcgtgg gccgcgtgga gatcccgcag gaagccttcc
    17161 tggcggtcct gcgcgttggc gacgaggagc agtgacgtga agcaccagaa ggcgaagacc
    17221 gccgccccag ccgcccccgc gccccccgcg cccgccgcgc cgcgccgcaa gtccacggtc
    17281 cgggagtact tcgaggctct cgccaccgcg gtgatcctcg cgctcttcgt ccgcaccttc
    17341 gtcgtccagg cgttcaagat ccccaccggg tcgatggaga acaacctgct catcggcgac
    17401 cacctgctcg tcaacaagat ggtgctcgcc ccctcgccgg ccgccgtcga ccgcctgctc
    17461 ctccccgtgc gcgacgtcag gcgcggcgac gtggtcgtct tcaagtaccc cgaggatccc
    17521 gagcgcgact tcatcaagcg ggtcatcggg ctgccgggcg agacgctcga ggtgcgcgac
    17581 aagcgggtct acgtggacgg ccgcccgctc gacgaaccct acgtccatta cctcgtgccc
    17641 ccctcgggct ccgcgcagtt caacgaggtg acctcgtacg acctgcgcga gcgctacgga
    17701 cccgtgacgg tgccgaccgg gcagtacttc gtcatggggg acaaccgcga caactcgcag
    17761 gacagccgct actggggctt tcttccccgc gagtacatca agggccgcgc cctgatgatc
    17821 tactggtcgt acgagtcgcg gagcgaggac tacgtggaaa acggcctcgc cgcttcgctg
    17881 cggcgcctgg tcaacgtcgc cacccacttc gtcacccgga cgcgctggag ccggatcttc
    17941 gaccccgtgc ggtaggcggg tctccccgtc aggctgctga aagcgcgccc cctcgctaca
    18001 ggcttctcct gcatcggccg agaagatcgg tggaagcgac gagtgcgccc ggagggcgcc
    18061 gcgcccggga gaacccatat ggtcggcaag ctggtcaaga tcgcgatcgc gctgctgctc
    18121 ctcaacggga tcgtgcggct gggtgcggcc tacctggccg actaccggtt cgaggaccgg
    18181 ctcacgcaga tcgcgcagtt cggcgatcgc cgcagcgagt cgcagctgtg cgccgaggcg
    18241 atcgaggcgg cccggcagtt cgaggtgccg gtcgcccccg aggacatcac catccgccgg
    18301 ggcacgaacc cgccgttcag ctgcggcaag ggatacaagg gcgccgtgtc ggtcagcagg
    18361 ggaggggccg agaagctgta catcgacgcg tcctaccagc gcgaggtcgc cctcctgccc
    18421 gggtacgccc ggcccttcac gttcaacccc tcggtcgagg tctgggcgag ggtgtactga
    18481 gaagcaggcg gagggcggga cgcgtcaggc gggaaggaag cctgacacta cgcggccgtt
    18541 cttgatgacc gcggcgatcg agggcgcgcc gaccctcaac aggtcgaccg cctcgccgtt
    18601 caccaccacc gcgtccatcg atttgccaac ctcgaggctg cccacccggt cgtgccggtc
    18661 gatcgcgtac gccgcgttca gggtggcggc gatcaaggct tcctcgagcg tcaatcgcat
    18721 cccgaagcag gccagggcca tcacgaacgg catcgacggc gtgaagccgc cgcccgggtt
    18781 gatgtcggtg gcgagggcaa ccgccacgcc gtgctcgatc agcatccgcg ccggcgcgaa
    18841 ccgccccatc ttcaagtaga aggcggcccc ggggagcagc gtcgcgacca cccccgcgcg
    18901 cgccatcgcc tcggccgagc caggctccac gaacagcagg tgatccgccg agcgcgcccc
    18961 gacgtcggcg gcaacgtcgg agccgccgct cgccgccatc tcgtcggcgt ggatgcgtgc
    19021 ccgcagcccc gcctcctggc ccgcccgcag gatctcgcgc gactcgacgg gggtgaacac
    19081 gccggtctcg cagaacacgt cgcaccactc ggcgaggcgc ccggccgcga cggccgggat
    19141 catctctcgc acgaggagat cgacgtactg ctgccggcgc tcgcggtact cgagcgggat
    19201 ctcgtgcgcc cccaagaagg tgggcgccag gtcgatcggc tgccgcgccg cgagcgtgcg
    19261 aatcgcccgg agcatccgca gctcggcggc ggtttcgagg ccgtatccgc tcttcgcctc
    19321 cgccgtcgtg gtgccgcacg ccagcatctc cgccaggcgc ggcaaggcgg ctgacaccag
    19381 ctcttcctcc gacgcctgcc gcgtcgcccg gacggtgctg aggatgccgc ccccttcggc
    19441 cgcgatttcg gtgtaggcgg cgccggccag gcggcggcgg agctcctcgc gccggtcgcc
    19501 ggcgtagacc acgtgcgtgt gcggatcgac gaagcccggc accacggtgc ggccccccgc
    19561 gtcgagcgcc gtcgcgccgg gtgccggcac gacttccgcg tcgcaggctg cggtggtgcc
    19621 ggcgaagacg attcggccgt cgagcgaggc gacggcgccg tcggcgatgg gggaaacctc
    19681 gttctgggcg gggccccggc ggggcgccgg cccggcgcag gtgagcagcc ggccggcgtg
    19741 ccgcaggatg aagtcggcgt tcacggcggc ggtcagctct ggccctgcgc cttctgcacc
    19801 tcggccgccg cgtcgagcgc gcgcatgatc ggtgacgcgg ccggcgtctg cggggccggc
    19861 tcggccacct ggcgcgggcg gtgcagcagg atcttgtcct cgcgctcctt ctggtcctgc
    19921 tcgcgcacga agtcgagggc gttccgcagg gcggtgatcg tggactgcag cgaggcctcg
    19981 acgtcccgcc gcttcagctt gaggccgtcg atttcccgct gcaggtcgtc gattcttccc
    20041 tgcgactgct gcagcaccag ctcggccctc gattcggcct cttcgatggt gcgcttggcg
    20101 tcctgctcgg cgtgctcgcg gatctcgtcg gcgagccgct gcgccgtcag cagggtgttc
    20161 cgcaggttcc gctcgtgctc ccggtgctcg gcgatgagcg actccatccg cgccagctcc
    20221 tggtgcagcc ggtcggtctc ccgcagcgcc cgctcgtagt cgtcggcgac ctcgttgagc
    20281 agcgcgtcca cttcgttgcg gtcgtagcct ctgaacacga cggtcagctt ctgctgccgg
    20341 aggtcgagcg gtgtcacctt catgagctgt ctcctgtaca cgcctgggtt gctgcctctt
    20401 ccgagtctcg gtcgttcacg cgttcgacgt tagcccgcgc gggccgaaga tcgccgttcc
    20461 cacgcggacg atcgtggcgc cttcctcgac ggccggctcg aggtcgtggc tcatgcccat
    20521 cgagaggtgg accgccgagg ccggcaggcg gcccgacgcg agcagttcgt cgcgcagcgc
    20581 gcgcaggcgg cggaagtacg gccgcgcggc ttcggcgtcc tcgaagaagg gcggcatcag
    20641 catcaggccc gccggccggg cggcgcggca cgaccgtgcc gcctcgagca cttggcccag
    20701 gttctcgggg ggaacgccga acttggtggc ctcgccggcg agatcgacct ggaccagcac
    20761 ctccgggcgc acgccggccg cgagggcggc gcggtcgagc gcctcgagca ggggtgcgct
    20821 gtcgatggaa tggacgaccg ggaacgaggt ggctgccttt cgcaccttgt tcgactgcag
    20881 gtgccccacc aggtgccagc gcacgggcag gtcggaggtc gcctcgatct tcgggagagc
    20941 ctcctggacc cggttctcgc cgaagtcgcg ctgtccggcg gcataggccg ccctgacgtg
    21001 cgaggccggg taggtcttcg agacggccac caggaggacg tcggacggat cgcggcccga
    21061 ggccttggcg gcgcgggcga tgcgcccgcg gacacggtca agacgcgcgg caaggtcggc
    21121 ctcgaggctc acgggtcgaa gcggcaggga ggcggtccgg agacggacgg gcggccgccg
    21181 gggacgccgg cggccgcgac agggcgggac ggtatcggga ccgtccctac ttcttcagct
    21241 gcgtcacgag ggccttcgcc tgctcggcat actggccttc gggggcgagc gtcaggtact
    21301 tctcgaacat accggccgcc tcggccaggt tgccgaggtt cacgtagcac atgcccagct
    21361 ggtagtgcga gtcggcgtgc tgcggattga gcttgatcgc ttcctcgaag tgcttcttgg
    21421 cgtcctccgc cttgcccgcg ttccaggcga tgacgccctg gttgtagagg gcgtccacac
    21481 cgccgccgcc gccggtggcg ccgcccgccg atgccagctc ggccgccttc tggctggccg
    21541 cctgggcgtc atcgaagcgc ttctgcgcgt tgtagacggt ggcgaggccg ttgtaggcct
    21601 ccacgtagtc ggccttcagc tcgatcgcct tcttgaagga ctcttcggcc ttgtcgtatt
    21661 ccttcttctg cgcctgcgcg aagccgatgt tgtagtagca gtcgtagcag ctcggcagca
    21721 gcgtgatggc ctcgttgaac ttggcgagcg cggtgtcgta gtcgttcgaa cggctggcgg
    21781 caacgccctc ctcgaacagc gttttcacgg cttccgcctt cgcggcgtct tccttggtca
    21841 ggccgaccga acccggcacc agcgcgaagt tcacctcggc cgcgtcgccg agccgcacgc
    21901 gcgcgtcgaa cgactgcgag ccgagcttct ccttctcggc agtgaccttg tagcggcccg
    21961 gcggcaggcc gatctggatg aactcgcctt tcttgttcgt cttgacctcg tagctgcgcg
    22021 agatgccgtc gaggaacgag atcgagacct tcgcgccgtc gacgggcttg ttctccccgt
    22081 ccacgacctt acctttcaac atcccggtgg actgggccca gccgggcgac gcgatcgcaa
    22141 cggccgccag caccgcaaga acaacgagca gcgcacgacg gccggagaga acgggaagca
    22201 tgagtcaacc tccgtctgat tgcaggatac gggcaagaca cggcggggac gccaccgcat
    22261 caccagttta cacgatcttg gggcgcgaac cgtcgtgtca acagggcttt aggggtggtc
    22321 agcgggcgaa gaaacgggtc aggtcgtcca ggcgtgaggt caccggcgcg ggcggcagcg
    22381 cgtcgaggaa gaccgccccg taccccttcg tcctgacccg cgggtcgagc agcgccagca
    22441 ccccgcgatc ctgccggtgc cggatcaacc gcccgaagcc ctgaaggagc gtcagcacgg
    22501 ccagcgggac ctggtactcc gtgaaggggc tctcgcccct cgcctggatc gcctcgatcc
    22561 gcgcggcggt gaccgggtcg gccggcgagg cgaacgggat cttgtccacg atcacgcagc
    22621 tcagcgcctc gcccaccacg tccacgccct gccagaagct cgacgtggcc agcagcacgg
    22681 cgttgccgag cgagcggaac cgccgcagca gcaccgagcg cggggcctgt ccctgcacga
    22741 gcaccgggaa ggggagtgaa gggggaagcg aacgctccac ctcgcgcagc atcgcgtagc
    22801 tggtgaagag gacgaaggcg cgcccctcgg tgcgctcgag gatctggagg atctcgcggg
    22861 ttgccgccgc cgggaattgc ggcgagcggg ggtcgggcat ccgcggcggc aggtagagga
    22921 tcgcctgctc ccggtacgcg aactcggagg gcacgcggat ttcgtcggcg tcccccactc
    22981 cgaggcggcc ccgcacgtag ccgaagctgc ccccgacggc gagggttgcc gaggtgagga
    23041 cggtggcagg cagccgatcg agcagcagct cccgcacgat cgccgacacg tcgatcggcg
    23101 aggcgcgaag cgcgatgccg cggccgcgcg tctcgaggta gtagacgaag tcggggtcgc
    23161 ccgcccggac gaggaaggcc aggtgatcgt gcacctcgcc cgcgcgacgg gcgatggccc
    23221 gcaggtcctc gggcgggctc ggggccaggg cgatctcccc ctggacctcg tcgagcgcct
    23281 cgcagagcga cgcggcgggc tgctgcacct cggcgagcgt ctcgggcgcc acgcgcgcgc
    23341 gctcctcccc gcgcaggttg cccctggcgc ggcgcagcgc gtcgaagaag cgcggcgctc
    23401 ggtcggccac ctcctcgagg ccgcgccgga tccccccgcg cgccgcctcg tcgaggctgg
    23461 cggccgccac cagcgcccgc gtggcgtcgt gcagcaggtc ctcgatccgg tacgtgctga
    23521 ccgcgactcc gaagtactgc gtcgccacct cctcgagctg gtgggcctcg tcgatcaccg
    23581 ccacctcgca ctcgggaatc accccgcccg ccgactcctg ccgcaccgag gcgtccgcgc
    23641 acagcaggtg gtggttgacg acgacgaggt ccgactgctc ggcgcgccgg cgcatccggg
    23701 tgacgaagca gtcttcgtgg cggggacagg acggtccgat gcagttctcc gacgaggccg
    23761 agagttcggc ccacacctcg aggtcgtcgg gcagcccctc gagttcggcg cgatcgccgg
    23821 tgtcggtctg acggccccac gacgcgagga ggtcggccac ctcgggctcg acgagcgcca
    23881 gcgacagcgg gttctcgcgg aactggtcga agcggtggag gcagaggtag ttgccgcgtc
    23941 ccttcaggca ggtggcggtg aagtcgatgc ccagggcccg gcgcaggtcc t
//
LOCUS       DAOPQX010000013        23503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2723_length_23503_cov_4.818748, whole genome shotgun sequence.
ACCESSION   DAOPQX010000013 DAOPQX010000000
VERSION     DAOPQX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 23503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23503
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2723_length_23503_cov_4.818748"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKK95_01510"
     CDS             <1..368
                     /locus_tag="PKK95_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16909.1"
                     /translation="LSLTYQPSEQWNQALTLTYASFERSRGGVRLYDYAIARSRTTFQ
                     FNRYLFFRGIVEYNSFRRQLVTDLLGSFTYIPGTVLHAGYGSLYERTRWDGFQYVRDD
                     RFQEARRGLFLKASYLWRM"
     gene            complement(373..1188)
                     /locus_tag="PKK95_01515"
     CDS             complement(373..1188)
                     /locus_tag="PKK95_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOC16910.1"
                     /translation="MVPGRRSHEIEFKIYGAEMQFVEVYLDPGESAVAEAGTMMFMTN
                     AIKMETVFGDGSAQQQGGVMGALLGAGKRLLTGESLFMTVFTNGSAGGVERVAFAAPY
                     AGKILPMDLAKLGGTLICQKDSFLCAAKGVSIGIAFQKKIGVGLFGGEGFIMQKLEGD
                     GLVFAHAGGTVHTYDLPAGQTLRVDTGCLVALQPSVNYDVQFVGGVKTALFGGEGLFF
                     ATLTGPGRVWLQSLPLSRMADRIYKAMPKMGKGGTEEGSIIGNIGLGGILGKS"
     gene            complement(1240..1392)
                     /locus_tag="PKK95_01520"
                     /pseudo
     CDS             complement(1240..1392)
                     /locus_tag="PKK95_01520"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
     gene            complement(1445..2155)
                     /locus_tag="PKK95_01525"
     CDS             complement(1445..2155)
                     /locus_tag="PKK95_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF533 domain-containing protein"
                     /protein_id="HOC16911.1"
                     /translation="MNIEHMIGEMVLGSLGGRRKRSRRAKRFLTGGGGFLNASTLLAL
                     GGVAWGVFETLSRQNQGGQAQPVPGGFPPPGVSPAPPLFSAGPVTPPPLPGAAAAPAA
                     PTAAPSGIPDGAVRIVRLMISAARADGTLSPDEREAILGQARGAGLEALAAGEVDHPT
                     PLEAIVSGIPDEAQRRDLYTLAFSIVRADEQVSGAERIYLARLASLLGLDPAAAADLE
                     RKASAGIDAADAPETSVP"
     gene            complement(2299..3462)
                     /locus_tag="PKK95_01530"
     CDS             complement(2299..3462)
                     /locus_tag="PKK95_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOC16912.1"
                     /translation="MGWNEEPDALADELNALAGSSDPRGESPAESRETLDPTRLRSWL
                     EQLARASGSDLLLVAGVPPSVRVDGDIRRLDEDPLEGEEIERAVLPALPAYGQRRYAE
                     SRIADAALAVPGLGRYRVNLHRERGRPAATIRALPSRVPRLSSLNLPAGVELLTRLAH
                     GLVLIGGATGSGKTTTLAALVDEINRRDRRHIVTIEDPVEYGHQHARSIVEQVEIGVD
                     APDFPTALRAAVRQAPDVIVVGEMRDPETMRIAVQAAGIGHLVLTTLHTTDVAAAVGR
                     IADNFPPERQPTIRQDLSMGLSAVLIQSLLPRIGGGRVPASELLVVGYGARQHIRKNA
                     LQHLNQEITITRKAGSYTLEEELASLVKRNLVSREDAQLRASHPEEFLKILAT"
     gene            3579..4619
                     /locus_tag="PKK95_01535"
     CDS             3579..4619
                     /locus_tag="PKK95_01535"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOC16913.1"
                     /translation="MTRHPPRLNAHRRASRPPVALAAAVLQVLALAVLALLCAAAPSP
                     AQDPLSAAKDHYAAAEYEKALGALDEAKKAPGVAPPTLLAIERYRALCLLALGRKPEA
                     ERAIEAVLDLDPFYVPGDDDAAPWVQAAFREVRRRVLPATLQQRYGRGKQAFERKEYA
                     AADRAFADVLRMLNDPDLALDEGAKSDMRLVVQGFLDLSKAASAVPPPPPAPLPEVAS
                     APATPKPRAASSEGASKPGGAARTPAQESKGGGPVYDSTSQDVVPPLPLRQSVVIPEQ
                     ARPREEIAGVVEIVIDTSGFIESAVLRQSFGSALDGLVLQAVKGWRYRPGMRAGNPVR
                     YRRLVRIVLPSR"
     gene            4627..6471
                     /locus_tag="PKK95_01540"
     CDS             4627..6471
                     /locus_tag="PKK95_01540"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOC16914.1"
                     /translation="MERLALALTLCAAGLGIAVVSAQRTVKPVLHAQHWLAITGKPLA
                     ATPGAMMFQKGGNAVDAACAMLGAASTMWDTLGWGGETQALIYNPKTGKVIGVNALGV
                     APTGATPEFYRSKGMAYPPEYGPLAAVTPGTPGGLLVMLAEYGRLSLADVLAPSIQMA
                     DGYPIEQQAADAIERYKDEIRKWPPSAAVFLTHPGEAREAPAAGEIFRQADLARTLRR
                     LVEAEQQALKAGRTRKEAIYAAYDRFYKGDLAEEFVRGSRELGGLITREDLASWKVHL
                     EEPVVTRYKGIDVYKLTTWVQGPVMLQALNMLEPMDLKGMGYNSARYIHTLYQVMNLA
                     FADRDFYYGDPYFPPEEPIEGLLSKEYAAARARLIDHEKNDPKVKPGDPYPFQGGTNP
                     YAHLLASWPPPPPKAAGTEGARAFEEGFRAGTTSIQAADEEGWVVSVTPSGAWIPATI
                     AGSTGIGMSQRMQAFVLDEAMGPFNVVAPGKRPRSTLTPGLALKDGAPYLSFAVQGGD
                     SQDQNLLQFFLNVVEFGMNVQQAAEAANFNSYQMHSSFDQHRSEPGRLLLHEAVPAWV
                     RDELRRKGYELEFEPLTSGPINAIFFDRAHRTMWGGSSHHGEDYGIGW"
     gene            6514..6786
                     /locus_tag="PKK95_01545"
     CDS             6514..6786
                     /locus_tag="PKK95_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16915.1"
                     /translation="MSMVGLVLLIACANVANLLMARTVIGIAVGVPASIAVSRLVQSQ
                     LFGVAAGDPATVAAASLLLLAVALLAGYVPAGQATRIDPVRALRAG"
     gene            complement(6992..8560)
                     /locus_tag="PKK95_01550"
     CDS             complement(6992..8560)
                     /locus_tag="PKK95_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOC16916.1"
                     /translation="MTCARRSTALVLAIVLSAAVAAAQPAAPPGFDAFVGRVMKTFEV
                     PGLSVAIVKDADTFVKGYGVRKLGEPAPVDAGTLFGIASNTKVFTAAALGLLVEEGKL
                     QWDGRVIDYLPWFQMYDPYVTRELTIRDLLVHRSGLGLGAGDLLWWPPSTYDRKQIAA
                     RLRHIKPVAGFRTAYAYDNVLYLIAGEVIEAISGQSWEDFVGSRLLAKAGMKGSNLKH
                     SDAALAGNVAATHARVEGIVRPITPFLSDNTNPAGGINSSAEDMAKWVRVLLAKGALP
                     DGTRLFSEATWRQLTTLVTPVPIGSVPQELAAISPSFNGYALGLGVRDYRGEKLLTHT
                     GGLPGYVSRVAWIPSRQLGVVVLTNQESSEAFQAIVHHVLDHHLGAKGTDWVETYRVL
                     AGRQQGAFDEAEKKAMAARNPSSRPSLPLPKYAGRYRDEWYGDVTLTEENGRLVMRFV
                     PTPSLAGTLEHFQYDTFIVRWNDRELRADAYVQFALKPDGSIDRVTMDAVSPMTDFSY
                     DFQDLLLVPVPSGK"
     gene            complement(8616..9800)
                     /locus_tag="PKK95_01555"
     CDS             complement(8616..9800)
                     /locus_tag="PKK95_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOC16917.1"
                     /translation="MSLIREKVAQAAALMRELDLPCWLTFVRETGILPEPVMPFLAPG
                     HVTWHSAFLVPPNGQAVAIVGLYDKKAIEETGAYARVEGYVEGYKAQLLAALRELDPP
                     AIAVNYSEESEVCDGLTHGMYLTLRRVLVEIGYEDRLVSSERLVAALRARKTPGELAR
                     IRRAIHETEAIFDEVGRFLAPGRTEQEVARLVTRLRTERGLEPAWEEATCPAVFTGPD
                     TAGAHYSPTARAVAGGHIVNMDFGVKFEDYCADLQRTFYVLREKEVEAPEAVMRGFQT
                     IVRAIETARRALRPGAVGRDVDRVARELVTAAGYDEFPHALGHQVGRFAHDGTALLGP
                     MWEKYGRRPLVPIEEGMVFTLEPRLTVEGHGVATVEEMVVVTREGAEFLSTPQTGLRL
                     AG"
     gene            complement(9844..10587)
                     /locus_tag="PKK95_01560"
     CDS             complement(9844..10587)
                     /locus_tag="PKK95_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC16918.1"
                     /translation="MPAARPRKPRGAPRSARLRRLADTAYTRLRESIVTLRLAPGAPL
                     TELDLCKRLGISRTPVRAALLRLEQEGLVTASPRRSGRMAVAPLTADDMRELFLMVGA
                     LDGVAARLAAELPEPARAAVVRTGHALNARLRELSTADLTDIRAAQDLDMRFHRCYEE
                     AAAGPRLRAKLDALHARRERYVRVYTEALLLTGTVEESLDEHLAILRAIGEGRPEEAE
                     RLATFNYRNGLERYRRIVAIHGERGNWNT"
     gene            complement(10649..13006)
                     /locus_tag="PKK95_01565"
     CDS             complement(10649..13006)
                     /locus_tag="PKK95_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOC16919.1"
                     /translation="MTERVARLRQLSLDTRPTLSTERAELMTEFYRQAPAVPLPILRA
                     RSLRHLMERKAIYIGEGELIVGERGPRPKATPTYPELCCHTLDDFEILDTREKISFAV
                     SDEARAAYRDKVIPFWHDRSIRHRMFEQLPQEWKDAYEAGVFTEFMEQRAPGHTVADD
                     KIYRKGLAGLQADIDRQLASLDFLNDLEAYDKKQQLLGMRLAADAVIRFAERHAEQAE
                     RLAAVEADPTRRAELELIAATCRHVPRQPPRTFWEALQAYWFVHLGVITELNTWDSFC
                     PGRLDQHLYPFYKREIEAGTLTRERARELLQCFWVKFNNQPAPPKVGVTAAESGTYTD
                     FSNIGLGGMKADGSDGVNEVTYLLLEVIDEMRLLQPSSNIQLSKKNPERFLRKACEVI
                     RKGWGQPSIFNADMVVAELVRQGKSVEDARCAGTSGCVEAGAFGKESYILTGYFNLPK
                     VLEITLNDGLDPRTGKTIGLETGDPAAFTAFDQVLDAFARQLRHFIDVKIAGNQIIER
                     LYAACMPAPFLSIITDDCIAKGRDYNNGGARYNTRYIMPVGPATTADSLSAIQYHVFD
                     RKTVTMERLVKATRDNFAGAEPLRQLLVNKTPKYGNDDEYADRFVERLCGMLVRDIDG
                     RRGPTGSTYHVNYLSTTCHVYFGSVCGATPDGRRAGAPVSDGISPAHGADRNGPTAVI
                     RSAARMDHARSGGTLLNQKFTPSLLDGDDGIEQLAHLVRSYFKLDGHHIQFNVVTADT
                     LRAAQKDPEKYRDLIVRVAGYSDYFCDLTKELQEEIIARTEQRQF"
     gene            complement(13015..13968)
                     /locus_tag="PKK95_01570"
     CDS             complement(13015..13968)
                     /locus_tag="PKK95_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOC16920.1"
                     /translation="MSTEAEGTRAAGRQVDEPGDPIGTILRIARFAIHDGPGIRTTVF
                     LKGCPLRCAWCHSPESQRAAPEFMPQPDRCIRCGSCTAACPHGVLPAALADSAAPAGC
                     TTCGACVEACPTGARELAGHPMTVDTLVALVERDRIFYDESGGGVTFSGGEPLLQPEF
                     VLEAAASCRAEGIHVAVDTCGHVDPDALLAVAREADLFLFDLKTVDEAAHRRLTGVPN
                     DLILSNLERLAAVHPRIIVRFPLIPGVNDDEANVRGIGALLASYRLTRVDVLPYHRAG
                     LAKYHRLQQPYALEEIEPPSPSHLAGVVKRLAGFGLIVGPS"
     gene            complement(13973..14746)
                     /locus_tag="PKK95_01575"
     CDS             complement(13973..14746)
                     /locus_tag="PKK95_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC16921.1"
                     /translation="MPNADLDHDPALAAFAGAPGAAGARGGNGGRQEVTAGAYRRVRE
                     LIVAGRLAPGAPLIETDLSRRLGVSRTPVRAALQRLHSEGFVAASRSGQMLRAFVSPL
                     TADDMREVFLMVGSLESVAARLAAALEPGRRAVLADRLAALDRELAAAAAVRPPDLVH
                     AQDLQVRFRQAFVQAASGPRLCAELDVLEAQAERYERVYTGAVLLAFEEGAAGRASIA
                     AAIGRGDAEAAERAVADYWRAAADRYGQVVSILGERGNW"
     gene            15028..16443
                     /locus_tag="PKK95_01580"
     CDS             15028..16443
                     /locus_tag="PKK95_01580"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOC16922.1"
                     /translation="MSTQRPITLGLIVGNRGFFPDHLARSGREEMIGVLERAGAKVVV
                     LGPEDAKHGAVTTREEARRCAELFDRHRKEIAGVIVTLPNFGEERAIVDALRQSGLRV
                     PVLVQATPDDTGSMTIADRRDSFCGKMSACNNLKQYGIPYSLTTLHTETPSSSEFAAD
                     LECFLGVCRVVQGLRNLRVGAVGARPAAFNTVRFSEKLLEAAGISVEPVDLSEILGRI
                     NRLADDHPAVKGKLAAIGAYAPTGAIPMASTLRMAKLGAVIDEWMAETEVTISALQCW
                     TALEEFYGIVPCTVMSMMSDELKSSACEVDILGAIAMHALRLASGTPSALLDWNNNYG
                     ADPDKAVCFHCSNLPKHFFADVQMDYQAIIAGTVGRDNTYGTLVGRVKPGPMTFARFS
                     TDDVKGRITGYVGEGEFTADPLTTFGGAGVVRIPRMQALLRHICENGFEHHVAANLSS
                     VAGIVYEAATKYLGWEMYWHR"
     gene            16543..18123
                     /locus_tag="PKK95_01585"
     CDS             16543..18123
                     /locus_tag="PKK95_01585"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOC16923.1"
                     /translation="MAIVAGVDFGTLSVRVSIVDSVRGRLGSGVGEYPLHRRRDDPDH
                     ATQSHHDQMEALVSATGLALARAGVRGDQVEALALDTTGSSVVPVGEGLQPLDDYYLW
                     CDHRAWREAALITKTAHERGLEAIHWSGGTYSSEWGFSKLLHWLRHNPDKRGRLLTAL
                     EHCDMVAATLCGITDPAEVPRSVCAMGHKWLWNASLGGLPPEEFLVAVDPLLAGVRDK
                     LNGRYLTSDHLAGRLAPEWAARLGFRPGIPIPVGAFDAHWDAIGAGVRLGDIVNVIGT
                     STCIMAVSEGTSLVPGVCGVVPGSVHPALTGIEAGLSATGDIFDAIARRAGTTVSKLS
                     DGLDRYRAGETGLLHLTWDNGDRTVLVNPELGGVTLGWNLTHTPLDHLFAAVEGTAFH
                     TRIILERMEEHGVSIDRVINGGGIPRKNRVLNAVYANVLNKPILVPRAEVTSLGSAIF
                     AFLAAGTFPSIEAAQKALCPEYEVVQPDPAQAAVYTELFPLYRALYFSLGRQGSEPAP
                     LGHVLPSLRRIAAEARRS"
     gene            18120..18815
                     /locus_tag="PKK95_01590"
     CDS             18120..18815
                     /locus_tag="PKK95_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOC16924.1"
                     /translation="MSGLDALRREVLEANLELVRRGLVVSSFGNASGIDREAGVVVIK
                     PSGVAYDQLTPEHLVVTDLEGHLVEGNLRPSSDLPTHIALYRAFPEIGGVAHTHSRHA
                     TAWAQAGREIPCLGTTHADYFPGPVPITEPMTAAEIRAAYEWNTGQVIVRRFASLNPS
                     HVPAVLVHGHAPFCWGPSPAAAAHMAVLLEEIAALAAITVSLNPSVEPLAAALRDKHF
                     RRKHGPEAYYGQK"
     gene            18848..21526
                     /locus_tag="PKK95_01595"
     CDS             18848..21526
                     /locus_tag="PKK95_01595"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOC16925.1"
                     /translation="MRKRSLHAAVLILLIVTGGLLRAPLGAQDSARLTEASLDGLTWR
                     NLGPFRAGTWISDIAVPERPPRAHLYTFYVALRYGGLWKTTNSGTTFELVFDEPDVTG
                     IGCVTIAPSNPDVIYVGTGDASSVRVAYPGNGVYKSVDAGKTWQRVGLEATSQIGRIV
                     VHPHDPDIVYVAAMGRLWSTGPDRGVYRSTDGGRRWKKVLYVDEETGAIDIVVNRRDP
                     DTLYAATYQVQRRPWRLYEGIGTGSGIHKTTDGGRTWKKLSGGLPTGPQGRIGLDLYQ
                     KSPNILYAVTENLALRPPTPAEIQRAKEQGVEPRERPIGGEVYRTDDGGRTWKKMNSA
                     DDDVSSKAGYSFNQIRVDQQNDRKILISCDSLISSEDGGKTFRGLDWSRRALFASAFG
                     DFRSMWIDPEDSNRMIMGTDGGVHVSYDGGKTVDHHYNLPGGEIYAIDVDMEDPYHIY
                     AGLQDHDSWKGPINGPHGRVGPEDWVTVGDNDGMYNRVDPTDSRWVYNSIQWGGQYRA
                     DQKTHTRKRIVPTRPAGQPPLRFNWTPPILLSPHNPAIVYTGAQVLFRSLDRGDHWQE
                     ISPDLTANDPEKISPPGMTVQFCTLTTLSESPLQAGVIWAGADDGKVQVTTNHGASWT
                     DVTPNLAAAGAPAHYWTTRVLASRFAAGTAYVTKSGRRYDEMKTVVMKTTDFGKTWAS
                     VAGNLPDRSLDVVVEDLEDEQILFVGSDRGVYVTLDGGRDWVAFKGNMPTVPVTDMVI
                     HPREHDLVAATYGRGLFVTNVAWLAEAKEGVLQKDAHLFAIRPRAVPREGPWGNFELY
                     GDRHLIVPNDDGVNVDFFLRSKPAEKVKVTIADGAGATVRTIEAEGRAGLNRVTWDVT
                     AGRGRRLGPGVYGVTVEAGGQTLAGTARVVPEGHPR"
     gene            21583..22971
                     /locus_tag="PKK95_01600"
     CDS             21583..22971
                     /locus_tag="PKK95_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOC16926.1"
                     /translation="MFDAQVYVGRRNGLQRQFQDGLLLFVGNADAPMNYAANAYHFRQ
                     DSSFLYYWGVDDPDLVAVLDVDAGRHVIYGDDFTVDDIVWRGPQPTIAERAGRAGVTE
                     TLPLKDLPRLMSEALRAGRRIHFLPQYRADNAQLLERLLGIRAEAINEHASVALCRAV
                     VSQRAIKSAEEIAEIERALEISRDMHVLAMRMAKPGMYEREVAGAMEGLVASRGVAMA
                     FPVIFSVHGETLHNHYHGNRMEAGQMAVNDSGAESPLHYASDITRTIPIGGRFEGPQR
                     DLYQAVHRAQKKAIAAVKPGVKFRDVHLLACRSLAEDLKTMGLMKGNLDAAIEQGAQG
                     MFFQCGLGHMLGLDVHDMEGLGEQHVGYDASVQRSAQFGLKSLRMARPLEPGMVMTVE
                     PGIYMIPVLMARWRAEGKFAEFLDYDAIERFRAFGGIRLEDDVLVTETGNRVLGQPIP
                     IEMEEVEALARQ"
     gene            23037..23297
                     /locus_tag="PKK95_01605"
     CDS             23037..23297
                     /locus_tag="PKK95_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16927.1"
                     /translation="MEPKHVVVGLHLTDRFQQATRVQSLLTEYGHQIKTRIGLHRTEE
                     LGTPQGVILLEIVGDDAGRVALVGKLREVPGVEVQEIVFEHC"
BASE COUNT         3609 a         8113 c         8274 g         3507 t
ORIGIN      
        1 cgctgtcgct gacctatcag ccctcggagc agtggaacca ggcgctcacg ctgacctacg
       61 ccagcttcga gcgcagccga gggggagtgc gcctctacga ctacgcgatc gcgcgcagcc
      121 gaaccacctt ccagttcaac cggtacctct tcttccgcgg catcgtcgaa tacaacagct
      181 tccggcgcca gctggtgacc gacctgctcg ggtcgttcac ctacatcccg ggcacagtgc
      241 tgcacgcggg atacggctcg ctgtacgagc ggacccggtg ggacggcttc cagtacgtcc
      301 gcgacgaccg cttccaagag gcgcggcggg ggctgttcct gaaggcgtcg tacttgtgga
      361 ggatgtagtg cgctagctct tcccgaggat tcctcccagc ccgatgttcc cgatgatcga
      421 gccttcctcg gtgccgccct tgcccatctt cggcatcgcc ttgtagatgc ggtcggccat
      481 gcggctgagc gggagcgact gcagccacac gcggcccggc ccggtgagcg tcgcgaagaa
      541 gagcccctcg ccgccgaaca gcgccgtctt cacgccgccg acgaactgca cgtcgtagtt
      601 caccgacggc tgcagcgcca cgaggcagcc ggtgtccact cgcagcgtct ggcccgccgg
      661 caggtcgtag gtgtggacgg tcccgcccgc gtgcgcgaac acgaggccgt cgccctcgag
      721 cttctgcatg atgaagccct cgccgccgaa caggccgacc ccgatcttct tctggaacgc
      781 gatgccaatc gacacgccct tcgcggcgca gaggaacgag tccttctggc agatgagcgt
      841 gccgcccagc ttggcgaggt ccatcggcag gatcttcccc gcgtaggggg ccgcgaacgc
      901 cacgcgttcg acgccgcccg cgctgccgtt cgtgaagacc gtcatgaaga ggctctcacc
      961 cgtcagcagc cgcttgccgg cgccaagcag cgcgcccatt acgccccctt gctgctgcgc
     1021 gctcccgtcg ccgaacaccg tctccatctt gattgcgttc gtcatgaaca tcatggtgcc
     1081 ggcctcggcc acggcgctct cgcccgggtc gaggtacacc tcgacgaact gcatctcggc
     1141 gccgtagatc ttgaactcga tctcgtgcga gcgccgtccg gggaccacgg gtggcggggc
     1201 ggtcgcgcca ccgccgaggt agcgtgccag ctgcggcacc tgctcgaggg gcgtccagtt
     1261 cgacatgccc ccggtgaaca ccagcgtctt gccgtcggcg gtgcccgact tcaagctggc
     1321 caccacttct tcctcgctca ccgggccgac ctggtgtccg ccaatcgcga ggaaccactg
     1381 ctgattgctc atgctgttac tctccagaga tcgagactcg tggatcacgg atcagggata
     1441 gggatcaggg tacggaggtt tccggggcgt cggccgcgtc gataccggcc gatgccttcc
     1501 tctcgaggtc ggccgcagcc gccgggtcga ggccgagcag cgacgccagg cgcgccaggt
     1561 agatccgctc ggcgcccgaa acctgctcgt ccgccctgac gatgctgaag gccagcgtgt
     1621 agagatcccg gcgctgcgcc tcgtccggga tgccggagac gatcgcctcg agcggcgtcg
     1681 gatggtcgac ctcccccgcg gccagcgcct cgaggccggc gccgcgagcc tgccccagga
     1741 tggcctcgcg ctcgtccggc gacaaggtgc cgtcggcgcg ggcggcggag atcatcaggc
     1801 ggacgatacg taccgcaccg tccggtatcc ccgaaggggc cgccgtgggt gccgccggcg
     1861 cggctgccgc gcccggcaga ggcggaggcg tgacggggcc ggccgagaac aacggcgggg
     1921 cgggtgagac gcccggcggc ggaaagccgc ccggcaccgg ttgggcctgc ccgccctggt
     1981 tctgacgcga cagagtctcg aagacgcccc acgccacgcc gcccagggcg agcagcgtgc
     2041 tggcgttcag gaagccaccc ccgccggtca ggaaccgctt ggcgcggcgc gacctcttcc
     2101 ggcgtccccc cagcgagccg aggaccatct ccccgatcat gtgctcgatg ttcatccggc
     2161 ttcttcctcc cgccgaggga cgaggatagc gcagaatcgc gatggggccg taccgtgagc
     2221 gtgagaaacg tcacgttctg ggtacggccc ctcgtgagtg aaagccgtca cacttttcaa
     2281 tacgtcctga gtgagggtct aggttgcgag gatcttcagg aactcctcgg ggtgcgaggc
     2341 ccggagctgc gcgtcctccc ggctcacgag gttccgcttc acgaggctgg caagctcctc
     2401 ctcgagcgtg tacgacccag ccttgcgcgt gatcgtgatt tcctggttca ggtgctggag
     2461 ggcgttcttg cggatgtgct ggcgcgcgcc gtaccccacc acgagcaact cgctggccgg
     2521 cacccgtcct ccgccaattc gtggcagcag cgactggatg agcaccgccg agaggcccat
     2581 ggacaagtcc tggcgaatcg tcggctggcg ctcgggcggg aagttgtccg cgatccgtcc
     2641 gactgcagcg gccacgtcgg tggtgtgaag ggtcgtcagc accaggtggc caatccctgc
     2701 cgcctggacc gcgatccgca ttgtctcggg gtcgcgcatc tcgccgacga cgatgacgtc
     2761 gggcgcctgg cggacggcgg cacgcagtgc cgtcgggaag tcgggcgcgt ccacgccgat
     2821 ctcgacctgc tcgacgatcg accgggcgtg ctgatgcccg tactcgaccg ggtcttcgat
     2881 ggtgacgatg tgacggcggt cgcggcggtt gatctcgtcc accagcgccg caagcgtggt
     2941 ggtcttgccc gagccggtcg caccgccgat caacacgagg ccgtgcgcga ggcgcgtgag
     3001 cagttcgacg cccgccggga ggttgaggct ggagaggcgg ggtacccgcg agggcagcgc
     3061 acggatcgtc gcggccgggc gcccgcgttc gcggtgcagg ttcacccggt accggccgag
     3121 cccaggaacc gcgagcgccg cgtcggctat gcggctctcc gcgtaccggc gctgcccgta
     3181 ggcgggcagg gccgggagca cggcacgttc gatctcctcg ccctcgagcg ggtcctcgtc
     3241 gagccggcgg atgtcgccgt ccacgcgaac gctgggcggg acgccggcga ccagcagtag
     3301 atcgctgccg gacgcgcgcg ccagctgctc gagccacgac cgaagacgcg tgggatcgag
     3361 cgtctcgcgc gactccgcgg gcgattcgcc acgcggatcg gaggatccgg ccagggcgtt
     3421 cagctcgtcg gcgagcgcgt cgggctcttc gttccagccc attcgaacct cccggtgggg
     3481 tcgggagtaa ttagaagtcg gaaagccggc acgacttcta attactcccg acccctgtct
     3541 ccggtcacgt cggtgctcgt atagaatgcc accatctcat gacaaggcac ccaccccgtc
     3601 tgaacgcaca ccgtcgagct tcgcggccgc cggtcgcgct ggcggccgcg gtgctgcaag
     3661 tcctggccct ggccgtgctc gcgcttcttt gcgccgccgc gccgtcgccg gcgcaggatc
     3721 ccctgtcggc agcgaaggac cactacgcgg cggccgagta cgagaaggcc cttggcgcgc
     3781 tcgacgaggc aaagaaggcg ccgggcgtcg cgcccccgac cctgctggcc atcgagcggt
     3841 accgggccct gtgcctgctc gccctcggcc ggaagcccga ggccgagcgg gccatcgagg
     3901 ccgtgctcga cctcgacccc ttctacgtgc ctggcgacga cgacgcggcg ccctgggtgc
     3961 aggctgcctt tcgcgaggtc cgccggcgcg tgctgcccgc gacgctgcag cagcgctacg
     4021 ggcgcggcaa gcaggcgttc gagcgcaagg agtacgcggc ggccgaccgg gcgttcgccg
     4081 acgtgctccg gatgctgaac gaccctgacc tcgcgctcga cgagggggcg aagtcggaca
     4141 tgcgtctcgt cgtgcagggg tttctcgatc tgtcgaaggc tgccagcgcc gttccgccgc
     4201 cgccaccggc gcccctgccc gaggtggcga gcgctcccgc gacgccgaag ccccgggccg
     4261 cgtcctccga gggcgcgtcg aagccggggg gcgccgcgcg gactccggcg caagagtcca
     4321 agggcggcgg ccccgtgtac gacagcactt cccaggacgt ggttccgccg ctgccgcttc
     4381 ggcagtcggt ggtcattccc gagcaggcac gcccacgcga ggagatcgcc ggcgtggtcg
     4441 agatcgtgat cgacaccagc ggcttcatcg agtcggcggt ccttcggcag tcgttcggct
     4501 cggcgctcga cggcctcgtg ctgcaggcgg tcaagggctg gcgctaccgt ccgggaatgc
     4561 gggccggcaa cccggtgcgg tatcgccgcc tcgtgcgcat cgttcttccc tcgaggtgac
     4621 acacccatgg aacgtctcgc tttggcgttg accctgtgcg cggccgggtt ggggatcgcg
     4681 gtggtgtcgg cgcagcgaac ggtgaagccg gttctccacg cccagcactg gctggccatc
     4741 accggcaagc cgctcgcggc gacaccgggc gccatgatgt tccagaaggg cgggaacgcg
     4801 gtcgacgcgg cctgcgcaat gctcggcgcc gcctcgacga tgtgggacac gctcgggtgg
     4861 ggcggcgaga cgcaggcgct gatctacaac ccgaagaccg ggaaggtgat cggcgtgaac
     4921 gcgctcggcg tcgcgccgac gggagcgacg ccggagttct accggtcgaa gggcatggcc
     4981 tacccgcccg agtacggccc gctcgccgcc gtcacaccgg gcacgccggg cgggctgctg
     5041 gtgatgctcg ccgagtacgg gcgcctgagc ctggccgacg tgctggcccc gtcgatccag
     5101 atggccgacg gctatcccat cgagcagcag gcggccgacg cgatcgagcg gtacaaggac
     5161 gagatcagga aatggccccc gtcagcggcc gtgttcctga cccatcccgg cgaggcgcgc
     5221 gaggcgcctg cggccggcga gatcttcagg caggccgacc tcgcgcgcac gctgcggcgg
     5281 ctggtggagg ccgagcagca ggcgctgaag gcggggcgca cgcgcaagga agcgatctac
     5341 gctgcctacg accgcttcta caagggcgac ctcgccgagg agttcgtgcg cggctcgcgc
     5401 gaactgggcg gactgatcac gcgcgaggat ctcgcgtcgt ggaaggtcca cctcgaggaa
     5461 cccgtcgtca cccgttacaa ggggatcgac gtctacaagc tgaccacctg ggtgcagggg
     5521 ccggtcatgc tgcaggcgct caacatgctc gagcccatgg acctgaaggg gatggggtac
     5581 aacagcgccc gctacatcca caccctctac caggtgatga acctcgcctt cgccgaccgc
     5641 gacttctact acggcgaccc gtacttcccg cccgaagaac cgatcgaggg gctgctctcg
     5701 aaggagtacg ccgcggcgcg cgcccgtctg atcgaccacg agaaaaacga tccgaaggtg
     5761 aagcccggcg acccgtatcc cttccaggga gggacgaatc catatgcgca tctgctcgcc
     5821 tcgtggccgc cgcctcctcc aaaggcagcg ggtaccgagg gggcgcgggc cttcgaggag
     5881 ggcttccggg cgggcacgac ctcgatccag gcggccgacg aagagggctg ggtcgtgtcg
     5941 gtgacgccga gcggcgcctg gatccccgcg accatcgccg gttccaccgg catcggcatg
     6001 agccagcgga tgcaggcctt cgttctcgac gaggcgatgg gaccgttcaa cgtcgtcgcc
     6061 cccggcaagc ggccgcgttc gacgctgacg cccggcctcg cgctgaagga cggtgcgcct
     6121 tacctctcgt tcgccgtgca ggggggcgac tcgcaggacc agaacctcct gcagttcttc
     6181 ctgaacgtgg tcgagttcgg gatgaacgtg cagcaggcgg ccgaggcggc gaacttcaac
     6241 agctaccaga tgcacagctc gttcgaccag caccgctcag agccggggcg cctgctgctg
     6301 cacgaggcgg tgcccgcgtg ggtgcgcgac gagctgcggc ggaagggcta cgagctcgag
     6361 ttcgaaccgc tgacctccgg gccgatcaac gcgatcttct tcgaccgggc acaccggacg
     6421 atgtggggcg gctcgagcca ccacggcgag gactacggga tcgggtggta gcgactgtcc
     6481 ggattcggga attcgccggc gcctccatct ccgatgtcga tggtcggcct ggtgctgctg
     6541 attgcctgcg cgaacgtggc gaacctgctg atggcgcgga ccgtcatcgg catcgcggtc
     6601 ggcgtgcccg cctcgattgc cgtcagccgc ctcgtgcagt cgcagttgtt cggcgtcgcg
     6661 gccggcgacc cggcgaccgt cgccgcggcc tcgctcctgc tgctggccgt cgccctgctc
     6721 gccggctacg ttcccgccgg ccaggccacc cgcatcgacc cggtgcgggc cctgcgcgca
     6781 gggtgatcgg acggggcagg gatcagatcg tgcaaaaggt cccgggggtg caaggggtgc
     6841 agaaggtgcg gtgctgatgg tgccggaagt gctggtgccg gaagtgctgg tgccgagagt
     6901 gccgcgcctg caccagcacc attggcacaa ccagcactgg cacccgcggc acattcggca
     6961 ccgtctgcac cttctgcacg actccgagac gtcatttacc gctaggaacc ggcacgagca
     7021 gcagatcctg gaaatcgtag ctgaagtcgg tcatcggcga gacggcgtcc atcgtcaccc
     7081 ggtcgatcga gccatccggc ttcaaggcga actgcacgta ggcgtcggcc cgcagctcgc
     7141 ggtcgttcca gcggacgatg aaggtgtcgt actggaagtg ctcgagcgtg ccggccagcg
     7201 acggggtcgg cacgaaccgc atcaccaggc ggccgttctc ctcggtcagc gtgacgtcgc
     7261 cgtaccactc gtcgcggtac cggccggcat acttcggcag cggcagcgac gggcgggacg
     7321 atgggttgcg agcggccatc gccttcttct cggcctcgtc gaacgccccc tgctgccggc
     7381 cggcgagcac ccggtaggtt tcgacccagt cggtcccctt cgcgccgaga tggtggtcga
     7441 ggacgtggtg cacgatcgcc tggaacgcct cgcttgattc ctggttcgtc agcaccacga
     7501 cgcccagctg cctcgaaggg atccaggcca cgcgggagac gtagccgggc agccccccgg
     7561 tgtgggtgag cagcttttcg ccgcggtagt cgcgtacgcc gagcccgagc gcatagccgt
     7621 tgaagctggg gctgatggcg gccagttcct gtggcaccga gccgatcggc accggcgtga
     7681 ccagcgtcgt cagctgccgc caggtggcct cagagaacag ccgcgtcccg tcgggcagcg
     7741 cgcccttcgc cagcagcacg cgcacccatt tcgccatgtc ctcggcgctc gagttgatgc
     7801 cgccggccgg gttcgtgttg tcactgagga acggggtgat cggccggacg atcccttcaa
     7861 cgcgcgcgtg ggtggccgcg acgttccccg cgagcgcggc gtccgagtgc ttcaggttgc
     7921 tgcccttcat cccggccttc gccaggaggc gcgagccgac gaagtcttcc cacgattgcc
     7981 cgctgatcgc ctcgatgacc tcgcccgcaa tgaggtagag gacgttgtcg tacgcgtacg
     8041 ccgtccgaaa gccggccacc ggcttgatgt gacgcaagcg ggcggcgatc tgcttgcggt
     8101 cgtaggtcga gggcggccac cagagcaggt cgccggcgcc gagccccagt ccgctgcgat
     8161 ggacgagaag gtcgcggatc gtcagctcgc gcgtcacgta cgggtcgtac atctgaaacc
     8221 acggcaggta gtcgatgacg cggccgtccc actgcagctt gccctcctcg accagcaggc
     8281 cgagcgccgc ggcggtgaac accttggtgt tcgaggcgat gccgaacagc gtgccggcgt
     8341 ccacgggcgc cggctcaccg agcttgcgca cgccgtagcc cttcacgaac gtgtcggcgt
     8401 ctttcacgat cgcgaccgag aggccgggga cctcgaaggt cttcatcacc cggcccacga
     8461 aggcatcgaa accgggcgga gcggctggct gggcggccgc gacggctgcc gacaggacga
     8521 tggcgagcac cagggccgtg gaacgtcgcg cgcacgtcat tgctgtttcc tcccggctga
     8581 gagccgctgg cgatgctaac acaggcgaaa ttccgtcagc ccgccagccg cagcccggtc
     8641 tgcggcgtcg agaggaactc ggcgccctcc cgggtgacga cgaccatctc ctcgaccgtc
     8701 gcaacgccat ggccctcgac cgtgaggcgc ggctcgagag tgaacaccat accttcttcg
     8761 atcgggacga gcggcctgcg cccgtacttc tcccacatcg gaccgagcag cgcggtgccg
     8821 tcgtgggcga agcgtccgac ctggtgcccg agagcgtgcg ggaactcgtc gtacccggcc
     8881 gcggtcacca attcgcgtgc cacccggtct acgtccctgc cgacggcgcc cggccgcagc
     8941 gcccggcgcg ccgtctcgat cgcgcgcacg atcgtctgga agccgcgcat caccgcctcg
     9001 ggcgcttcta cctctttttc gcggagcacg tagaaggtgc gctgcaggtc ggcgcagtag
     9061 tcctcgaact tcacgccgaa gtccatgttc acgatgtggc cgccggcgac cgcgcgggcg
     9121 gtggggctgt agtgggcgcc ggccgtgtca gggccggtga agaccgctgg gcaggtcgct
     9181 tcctcccagg cgggttccag cccgcgctcc gtgcgcagcc gggtgacgag ccgggcaacc
     9241 tcctgctcgg tgcggccggg agcgaggaaa cgtcccacct cgtcgaagat ggcctcggtc
     9301 tcgtggatcg cgcgccggat gcgcgccagc tcgccgggcg tcttccgcgc gcgcagcgcg
     9361 gcgacgaggc gttcggacga gaccagccgg tcttcgtacc cgatctccac aagcacgcgg
     9421 cgaagcgtca ggtacatccc gtgcgtcaac ccgtcgcaaa cctcgctctc ctcggagtag
     9481 ttgaccgcga tggcgggggg atcgagctcg cgcagcgcgg cgaggagctg cgccttgtag
     9541 ccctcgacgt aaccttccac ccgcgcgtaa gcaccggttt cctcgatggc cttcttgtcg
     9601 tagagcccga cgatggcgac ggcctggccg ttgggaggca cgagaaaggc gctgtgccag
     9661 gtgacgtggc cgggcgcgag gaacggcatc acgggctcgg gcaggatgcc ggtctcgcgc
     9721 acgaaggtga gccagcaggg caggtcgagc tcgcgcatca gcgcggccgc ctgggcgact
     9781 ttctcgcgga tcaaggacat gagcacctgc cttcgcaacg ccgggaacct tcaggagatc
     9841 acgttacgtg ttccagttcc cccgctcccc atggatcgcc acgatgcgcc ggtaccgctc
     9901 caggccgttg cggtagttga aggtcgcgag gcgctcggcc tcctctgggc ggccctcgcc
     9961 gatggcgcgc aggatcgcca ggtgctcgtc gagcgattcc tcgacggtgc cggtgaggag
    10021 cagcgcctcc gtgtagacgc gcacgtaccg ttcgcgccgg gcgtgcaggg cgtcgagctt
    10081 ggccctcagg cgcggccccg cggccgcctc ctcgtaacac cggtggaacc gcatgtcgag
    10141 gtcctgcgcg gcgcggatgt cggtcaggtc ggcggtggac agctcgcgca ggcgggcgtt
    10201 cagcgcgtgg ccggtgcgca cgacggccgc ccgcgccggc tcgggcagtt cggccgccag
    10261 gcgtgccgcg acaccgtcga gggccccgac catcaggaac agctcgcgca tgtcgtcggc
    10321 ggtcagcggc gcgacggcca tccggcccga ccggcggggc gacgcggtga cgagcccttc
    10381 ctgctccagg cgcagcagcg ccgcgcgcac cggcgtgcgg ctgatgccga gccgcttgca
    10441 gagatccagc tccgtcaggg gagcgccggg agcgaggcgc agagtgacga tgctctcgcg
    10501 caggcgggtg taggccgtgt cggcgagccg ccgcaggcgg gccgagcgcg gggctccgcg
    10561 gggcttgcgg gggcgggcgg cgggcatagg gcgcctattc tataaccgga gtcggacgcc
    10621 gcgcgacccc ggccggcgcc acccgagcct agaactgccg ctgctcggtg cgcgcgatga
    10681 tctcctcctg caactccttc gtcaggtcgc agaagtagtc gctgtagccg gccacccgca
    10741 cgatgaggtc gcggtatttc tcagggtcct tctgggccgc gcgcagcgtg tcggccgtca
    10801 ccacgttgaa ctggatgtgg tggccgtcca gcttgaagta cgaccgcacc aggtgtgcga
    10861 gctgctcgat gccgtcgtca ccatcgagca gcgagggggt gaacttctga ttcaacagcg
    10921 tgccgccgct gcgcgcgtgg tccatgcgcg ccgccgagcg gatgaccgcg gtcgggccat
    10981 tccgatcggc gccgtgggcc ggcgagatgc cgtcggagac cggcgccccc gcccggcgtc
    11041 cgtcgggggt cgccccgcac accgagccga agtacacgtg gcaggtggtg gacaggtagt
    11101 tcacgtggta ggtcgagccg gtcgggccgc ggcggccgtc gatgtcgcgg acgagcatcc
    11161 cgcagagccg ctccacgaac cggtcggcgt actcgtcgtc gttgccgtac ttcggggtct
    11221 tgttgacgag cagctggcgc agcggctcgg cgccggcgaa gttgtcgcgc gtcgccttca
    11281 ccagccgctc catcgtcacg gtcttccggt cgaacacgtg gtactggatg gccgagaggc
    11341 tgtcggccgt cgtggccggg ccaaccggca tgatgtagcg ggtgttgtag cgcgcgccgc
    11401 cgttgttgta gtcgcgcccc ttcgcgatgc agtcgtcggt gatgatggag aggaacggcg
    11461 ccggcatgca cgcggcgtac agccgctcga tgatctggtt gccggcaatc ttcacgtcga
    11521 tgaagtgccg gagctggcgc gcgaaggcgt ccagcacctg gtcgaaggcg gtgaaggccg
    11581 ccgggtcgcc cgtctcgagg ccgatggtct tgcccgtgcg cgggtcgaga ccgtcgttga
    11641 gggtgatttc gagcaccttc gggaggttga agtacccggt gaggatgtag ctctccttgc
    11701 cgaacgcccc cgcctcgacg cagccgctcg tgccggcgca gcgcgcgtcc tcgacgctct
    11761 tcccctggcg caccagctcg gcgaccacca tgtcggcgtt gaagatcgag ggctggcccc
    11821 agcccttgcg gatcacctcg cacgccttgc gcaggaagcg ttcggggttc ttcttgctca
    11881 gctggatgtt cgacgagggc tgcagcagcc gcatctcgtc gatcacctcg agcagcaggt
    11941 aggtgacctc gttcaccccg tcgctgccgt cggccttcat gccgccgagg ccgatgttcg
    12001 agaagtcggt gtaggtgccg ctctcggcgg ccgtgacccc caccttcggc ggcgccggct
    12061 ggttgttgaa cttcacccag aagcattgca gcagctcgcg cgcccgctcg cgggtgagcg
    12121 tgcccgcttc gatctcccgc ttgtagaacg ggtagaggtg ctggtcgagg cgccccgggc
    12181 agaacgagtc ccaggtgttc agctcggtga tgacgccgag gtgcacgaac cagtacgcct
    12241 gcagcgcctc ccagaaggtg cgcggcggct ggcgcggcac gtgccggcag gtcgcggcga
    12301 tcagctccag ctccgcccgc cgggttgggt cggcctcgac ggcagccagg cgctcggcct
    12361 gctcggcgtg ccgctccgcg aagcggatca ccgcgtcggc cgccagccgc atcccgagca
    12421 gctgctgctt cttgtcgtag gcctcgaggt cgttcaggaa atcgagcgag gcgagctggc
    12481 ggtcgatgtc ggcctgcagc cccgccagcc ccttgcggta gatcttgtcg tcggccaccg
    12541 tgtgcccggg cgcgcgctgc tccatgaact cggtgaagac gccggcctcg taggcgtcct
    12601 tccactcctg ggggagctgc tcgaacatcc ggtggcggat cgagcggtcg tgccagaacg
    12661 ggattacctt gtcgcggtac gccgcccgcg cctcgtccga cacggcgaac gaaatcttct
    12721 cgcgcgtgtc gaggatctcg aagtcgtcga gcgtgtggca gcacagctcg gggtacgtgg
    12781 gggtggcctt cggccgcggg ccgcgctcgc cgacgatcag ctccccctcg ccgatgtaga
    12841 tcgccttgcg ctccatcagg tgccgcagcg accgcgcgcg caggattggc agcggcacgg
    12901 caggcgcctg gcggtagaac tccgtcatca gctcggcccg ctcggtcgag agcgtggggc
    12961 gggtgtcgag gctcagctga cgaaggcggg cgacgcgctc ggtcatgacg gtcctcacga
    13021 tggtccgacg atcaacccga aacccgcgag gcgcttcacg acgcccgcaa ggtgcgaggg
    13081 ggacggcggc tcgatctcct cgagcgcgta cggctgctgc agccggtggt acttcgcgag
    13141 gcccgcgcgg tggtacggca gcacgtccac gcgcgtcagc ctgtacgacg cgagcagcgc
    13201 cccgatcccc ctcacgttcg cctcgtcgtc gttcacgccc gggatcaggg ggaagcggac
    13261 gatgatgcgg ggatggacgg cggcgaggcg ctccaggttc gacaggatca ggtcgttcgg
    13321 cacgccggtg aggcgccggt gcgcggcctc gtccacggtt ttcaagtcga acaggaagag
    13381 gtcggcctcg cgtgcaaccg cgagcagcgc gtcgggatca acgtggccgc aggtgtccac
    13441 ggcgacgtgg atgccttccg cgcggcacga ggcagcggcc tcgaggacga actcgggctg
    13501 gaggagcggc tcgccgcccg agaaggtgac gcctcctccc gactcgtcgt agaagatgcg
    13561 gtcgcgctcg acgagcgcca ccagcgtgtc caccgtcatc gggtggccgg ccagctcgcg
    13621 cgcgccggtg gggcaggcct cgacgcaggc gccgcacgtg gtgcagccgg ccggcgccgc
    13681 cgagtcggca agcgccgccg ggagcacgcc gtgcgggcag gcggcggtgc acgagccgca
    13741 gcggatgcag cggtcgggct gcggcatgaa ctcgggggcc gcgcgctggc tctcggggct
    13801 gtggcaccag gcgcagcgga gcgggcagcc cttgaggaag acggtggtgc ggatgccggg
    13861 gccgtcgtgg attgcgaacc gcgcgattcg caggatcgtc cctatcggat cgccgggctc
    13921 gtccacctgc cggcccgccg cgcgtgtgcc ctccgcctcg gtgctcatcg cgtcaccagt
    13981 tgccccgttc cccgaggatc gacaccacct gcccgtagcg atcggccgcc gcccgccagt
    14041 aatccgcaac cgcccgctcg gctgcctcgg cgtcgccgcg gccgatcgcg gcggcgatcg
    14101 aggcgcggcc cgccgccccc tcttcgaagg caaggaggac ggcgccggtg tagacacgct
    14161 cgtaccgctc ggcctgcgcc tccagcacgt cgagctcggc gcaaaggcgg ggcccggagg
    14221 ccgcctggac gaacgcctgg cggaaacgga cctgcaggtc ctgcgcgtgc accaggtcgg
    14281 gtggtcgaac ggcggccgcc gccgccagct cacggtcgag cgccgccagc ctgtcggcca
    14341 acacggcacg gcgcccgggc tcgagcgcag cggcgaggcg cgcggccacc gactcgagcg
    14401 agccgaccat gagaaagacc tcgcgcatgt cgtccgcggt caggggcgag acgaaggcgc
    14461 ggagcatctg ccccgagcgg gacgccgcca cgaacccttc cgagtggagg cgctggagcg
    14521 ccgcccgcac cggggtgcgg ctgaccccaa gccgcctgga gaggtcggtc tcgatgagcg
    14581 gggcgccggg ggcgagccgg cccgcgacga tcagctcgcg cacgcgccgg taggcgccgg
    14641 cggtgacctc ctggcggccg ccgttgccgc cccgggcgcc agcagcgccg ggggcgccgg
    14701 cgaaagccgc gagcgccggg tcgtggtcga ggtcggcgtt cggcatgggc ggatatcgta
    14761 gccgcggggt gcagggggcg caacgaggaa ccggctgcgg cgcagcatca gcatatagcc
    14821 gcctgcgacg tgttgtcgcc acaattgttc gtcgcgatgt atacaattgc aatcgcttac
    14881 aatcttggcc cagcgggcag atgatgcacg ccgggcagaa atagggcaca atccgccgag
    14941 tcccccaggc gcccccctcg acccggtagg tcccggcacc cgccgtgtct ggcgaggcgt
    15001 gccttttatg gaacggatga gcagcacatg agcacacaga gacccatcac cctcggcctc
    15061 atcgtcggca accgtggctt cttccccgac cacctggcgc gcagcgggcg cgaggagatg
    15121 atcggcgtgc tcgagcgcgc cggcgcgaag gtcgtcgtgc tcggccccga ggacgccaag
    15181 cacggcgcgg tgaccacccg ggaggaggcc cggcggtgcg ccgagctgtt cgaccggcac
    15241 cggaaggaga tcgccggcgt catcgtcacg ctgccgaact tcggcgagga gcgggcgatc
    15301 gtggacgccc tgcgccagtc gggtctgcgc gtgccggtgc tcgtgcaggc gacgcccgac
    15361 gacaccggct cgatgacgat cgccgaccgg cgcgacagct tctgcgggaa gatgtcggcg
    15421 tgcaacaacc tgaagcagta cggcatcccg tactcgctca ccacgctgca caccgagacc
    15481 ccgtcttcgt ccgagttcgc cgccgacctc gagtgcttcc tcggtgtgtg ccgggtggtg
    15541 cagggcctgc gcaacctgcg ggtgggggcg gtgggcgccc ggcccgcggc cttcaacacc
    15601 gtccgcttca gcgagaagct cctcgaggcg gccggcatct cggtggagcc ggtggacctc
    15661 tctgagatcc tgggccgcat caaccgcctg gccgacgacc acccggcggt caaggggaag
    15721 ctggccgcga ttggggccta tgcgccgacc ggcgccatcc cgatggcctc caccctgcgc
    15781 atggccaagc tcggtgcggt gatcgacgag tggatggccg agaccgaggt caccatcagc
    15841 gcgctccagt gctggacggc gctcgaggag ttctacggca tcgtcccctg caccgtcatg
    15901 agcatgatga gcgacgagct gaaatcgagc gcctgcgagg tggacatcct cggcgcgatc
    15961 gcgatgcacg ccctgcgcct cgcctcgggg acgccgagcg cgctgctcga ctggaacaac
    16021 aactacggcg ccgaccccga caaggcggtc tgcttccact gcagcaacct gccgaagcac
    16081 ttcttcgccg acgtccagat ggactaccag gcgatcatcg cgggcacggt gggtcgcgac
    16141 aacacgtacg gcacgctcgt cggccgcgtg aagccggggc cgatgacctt cgcccgcttc
    16201 tcgactgacg acgtgaaggg gcggatcacc ggctacgtcg gggaagggga gttcaccgcc
    16261 gacccgctca ccaccttcgg cggcgcgggc gtcgtccgca tcccgcggat gcaggcactg
    16321 ctgcgccaca tctgcgagaa cggcttcgag caccacgttg cggccaacct gtcgtcggtg
    16381 gccggcatcg tgtacgaggc ggcgacgaag tacctcggat gggagatgta ctggcacagg
    16441 taggaatcag gattcaggaa tcaggattcg ggaatcaggt tcaggaatcg gaccgctccg
    16501 aatcccgaat cctgatcccg agtcccttcg ggagaccctg acatggccat cgtcgcgggc
    16561 gtcgatttcg gaacgctgag cgtgcgggtg tcgatcgtgg acagcgtgcg gggacgcctc
    16621 gggtcgggcg tgggcgagta cccgctccac cggaggcggg acgatccgga ccacgccacg
    16681 cagagccacc acgaccagat ggaagcgctc gtctcggcga ccgggctcgc ccttgcccgc
    16741 gccggggtgc gcggcgacca ggtcgaggcg ctcgcgctcg acaccaccgg gtcgagcgtc
    16801 gtgccggtgg gcgaggggct gcagccgctc gacgactact acctctggtg cgaccaccgg
    16861 gcgtggcgcg aggcggcgct catcacgaag accgcgcacg agcggggcct cgaggccatt
    16921 cactggtcgg gtggcaccta ctcgagcgag tggggcttct cgaagctgct gcactggctg
    16981 cgccacaacc cggacaagcg cggccggctg ctgactgccc tcgagcactg cgacatggtg
    17041 gccgccacgc tgtgcggaat caccgatccc gccgaggtgc cccgcagcgt gtgcgcgatg
    17101 gggcacaagt ggctctggaa cgcgtcgctc ggcggcctgc cccccgagga gttcctcgtc
    17161 gccgtggacc cgctgctcgc cggggttcgc gacaagctca acgggcgcta cctgacctcc
    17221 gaccacctgg ccgggcgcct ggcgccagag tgggccgcgc gcctcggctt ccgtcccggc
    17281 atcccgattc cggtcggcgc cttcgatgcc cactgggacg cgatcggcgc cggcgtccgc
    17341 ctcggcgaca tcgtcaacgt gatcggcacc tcgacctgca tcatggcagt cagcgagggg
    17401 acctccctgg tgcccggcgt ctgcggggtg gttccagggt ccgttcaccc ggcgctcacc
    17461 ggcatcgagg caggcctctc ggcaaccggc gacatcttcg acgcgatcgc gcgccgggcg
    17521 ggtaccaccg tgtcgaagct ctccgacggg ctcgaccgct accgggcagg cgagacgggc
    17581 ctgctgcacc tgacctggga caacggcgac cggaccgtgc tcgtgaaccc cgagctgggc
    17641 ggcgtcacgc tcggctggaa cctgacccac acgcctctgg accacctgtt cgcggctgtc
    17701 gaggggaccg cgttccacac ccggatcatc ctcgagcgga tggaggagca cggcgtctcg
    17761 atcgacaggg tcatcaacgg cggcggcatc ccgcggaaga accgggtgct gaacgccgtc
    17821 tacgcgaacg tgctgaacaa gccaatcctc gtgccccgag ccgaggtgac cagccttggg
    17881 tcggccatct tcgcgttcct ggccgccggg acgttcccgt cgatcgaggc ggcgcagaaa
    17941 gcgctctgcc ccgagtacga ggtcgtgcag cccgacccgg cgcaggcggc ggtctacacg
    18001 gagctgttcc cgctctaccg ggccctttac ttctccctcg ggaggcaggg gtcggagccg
    18061 gcccccctcg gccacgtgct gccgtccctc aggcgcattg ccgccgaagc gaggcgatcg
    18121 tgagcgggct cgacgcgctg cggcgcgagg tgctcgaggc gaacctcgag ctggtgcggc
    18181 gcggcctggt ggtgtcgtcg ttcggcaacg cgagcggcat cgaccgcgag gccggcgtgg
    18241 tcgtgatcaa gcccagcggc gtggcgtacg accagctgac ccccgagcac ctggtcgtca
    18301 ccgacctcga gggacacctc gtcgagggca acctccgccc ctcgtcggac ctgccgacac
    18361 atatcgccct ctaccgtgcc ttcccggaga tcggcggggt cgcccacacc cactcgcgcc
    18421 acgccaccgc ctgggcgcag gcggggcgcg agatcccctg cctgggaacc acgcacgccg
    18481 actacttccc ggggccggtc ccgatcaccg agccgatgac tgcggccgag atccgcgcgg
    18541 cctacgaatg gaacaccggg caggtgatcg tccgccgctt cgcctcgctg aacccctcgc
    18601 acgtgcccgc ggtgctcgtc cacggccacg cgccgttctg ctgggggccg tcccccgccg
    18661 cggccgcgca catggcggtg ctcctcgagg agattgcagc cctggcggca atcaccgtgt
    18721 cgctgaaccc ctcggtcgag ccgctcgcgg ccgccctgcg cgacaagcac ttcaggcgca
    18781 agcacgggcc cgaggcgtac tacgggcaga agtagggggg gacgagcatc cttcaccggt
    18841 gttgcgcatg cgcaagaggt ctcttcacgc cgctgtcctc atcctcctca tcgtcacggg
    18901 cgggctgctc cgggcgccgc tcggcgccca agactctgcg aggctgaccg aagcatcgct
    18961 cgacggcctc acctggcgca acctcgggcc gttccgcgca ggtacgtgga tctccgacat
    19021 cgcagtgccc gagcggcctc cgcgcgcgca cctctacacc ttctacgtcg ccctgcgcta
    19081 cggcggtctc tggaagacca ccaacagcgg caccaccttc gagctggtgt tcgacgagcc
    19141 cgacgtcacc ggcatcggct gcgtgacgat cgcgccgtcg aatcccgatg tcatctacgt
    19201 cgggacgggc gacgcctcga gcgtgcgtgt ggcgtatccc gggaacggcg tctacaagtc
    19261 ggttgacgcg ggcaagacgt ggcagcgcgt ggggctcgag gccaccagcc agatcggccg
    19321 catcgtcgtc catccgcacg accccgacat cgtctacgtg gccgcgatgg ggcgactctg
    19381 gtcgaccggc cccgaccggg gcgtttacag gtccaccgac ggcgggaggc gctggaagaa
    19441 ggtgctctac gtggacgagg agacgggcgc gatcgacatc gtcgtcaacc gccgcgaccc
    19501 cgacaccctc tatgccgcca cctaccaggt gcagcgccgg ccgtggcgcc tctacgaggg
    19561 gatcggcacc ggcagcggca tccacaagac gaccgacggc gggcgaacct ggaagaagct
    19621 gtccggcgga cttcccacgg gcccgcaggg gcgcatcggc ctcgacctct accagaagag
    19681 tccgaacatc ctctacgcgg tgaccgagaa cctcgccttg cgcccgccca cgcctgccga
    19741 gatccagcgg gccaaggagc agggtgtcga gccgcgcgag cgccccatcg gcggcgaggt
    19801 ctatcgaacc gacgacggcg gccgtacgtg gaagaagatg aactcggccg acgacgacgt
    19861 cagctcgaag gccgggtact cgttcaacca gattcgagtg gaccagcaga acgacaggaa
    19921 gatcctgatc agctgcgact cactcatcag ttcggaggac ggcggcaaga cgttccgggg
    19981 gctcgactgg tcgcgccgcg ccctgttcgc gagcgccttc ggcgacttcc gctcgatgtg
    20041 gatcgacccc gaggactcga accggatgat catgggcacc gacggcggcg tccacgtgtc
    20101 gtacgacggc gggaagacgg tggatcacca ctacaacctg ccgggcggcg agatctacgc
    20161 gatcgacgtg gacatggaag acccttacca catctacgcg gggctgcagg accacgactc
    20221 gtggaagggg ccgatcaacg ggccgcacgg ccgggtcggg cccgaagact gggtgacggt
    20281 cggcgacaac gacgggatgt acaaccgcgt cgacccgacc gacagccgct gggtctacaa
    20341 cagcatccag tggggcgggc agtaccgcgc cgaccagaag acgcacacgc gtaaacggat
    20401 cgtcccgacc cggcccgccg ggcagccccc gctgcgcttc aactggacgc cgcccatcct
    20461 gctctcgccc cacaatccgg ccatcgtcta cacgggcgcc caggtgctgt tccgttcgct
    20521 cgaccggggc gaccactggc aggagatcag cccggacctc acggccaacg accccgagaa
    20581 gatctcgccc ccggggatga ccgtgcagtt ctgcaccctc accacgctct ccgagtcgcc
    20641 cctgcaggcc ggtgtcatct gggctggggc cgacgacggg aaggtgcagg tgacgacgaa
    20701 ccatggggcg tcgtggacgg acgtgacgcc gaacctggcg gccgccggcg cgcccgccca
    20761 ctactggacg acccgcgtgc tcgcctcgcg cttcgcggcg gggacggcgt acgtgacgaa
    20821 gagcggcagg cgctacgacg agatgaagac ggtggtgatg aagaccaccg acttcggcaa
    20881 gacctgggcc tcggtcgcgg gcaaccttcc cgatcggtcg ctggacgtcg tcgtcgagga
    20941 tctcgaggac gagcagatcc tgttcgttgg gagcgaccgg ggcgtgtacg tcacgctcga
    21001 cggcgggcgc gactgggttg ccttcaaggg caacatgccc accgtcccgg tgaccgacat
    21061 ggtcattcac ccgcgcgagc acgacctggt ggcggccacc tacggccgcg ggctgttcgt
    21121 caccaacgtc gcctggctcg ccgaggcaaa ggagggagtc ctccagaagg acgcacacct
    21181 gttcgccatc cgcccgcgcg ccgtgccgcg cgaggggccg tggggcaact tcgagctgta
    21241 cggcgaccgg cacctgatcg tgccgaacga cgatggcgtc aacgtggact tcttcctgag
    21301 gtcgaagccc gcggagaagg tgaaggtgac gatcgcggat ggcgcggggg cgacggtccg
    21361 cacgatcgag gccgagggac gggccgggtt gaaccgggta acctgggatg tgaccgccgg
    21421 gcgcggccgg cggctgggcc ccggggtcta cggcgtaacg gtcgaggcag gcgggcagac
    21481 cctggccggg acggccaggg tggtgccgga gggacatccg aggtgacgct cgaaatgcag
    21541 aggcacagga tccccgtatc ctgaactccc gaggagacgc gaatgttcga cgcgcaggtg
    21601 tacgtcggcc gccggaatgg gctgcagcgg cagttccagg acggcctgct gctgttcgtg
    21661 gggaacgcgg acgcgccgat gaactacgcg gccaacgcgt accacttccg gcaggattcc
    21721 tcgttcctct actactgggg cgtcgacgat ccagacctgg tggccgttct cgacgtggac
    21781 gcggggcggc acgtcatcta cggcgacgac ttcaccgtgg acgacatcgt ctggcggggg
    21841 ccgcagccga ccatcgccga gcgcgccggc agggccggcg tgaccgagac cctgccgcta
    21901 aaggatctac cgcgcctgat gagcgaggcg ctccgggccg ggcggcgcat ccatttcctg
    21961 ccgcagtacc gtgccgacaa cgcgcagctg ctcgagcggc tgctcgggat ccgggccgag
    22021 gccatcaacg agcacgcgtc ggtggcgttg tgccgggccg tcgtgtcgca gcgggcgatc
    22081 aagagcgccg aggagatcgc cgagatcgag cgggcgctcg agatcagccg cgacatgcac
    22141 gtcctggcga tgcggatggc gaagcccggc atgtacgagc gcgaggtggc gggcgcgatg
    22201 gaggggctcg tcgcgtcgcg cggcgtcgcg atggccttcc cggtgatttt ctcggtgcac
    22261 ggcgagacgc tgcacaacca ctaccacggc aaccggatgg aagccgggca gatggcggtg
    22321 aacgactcgg gcgccgagtc gccgctccac tacgccagcg acatcacccg gacgatcccg
    22381 atcggcgggc gcttcgaggg gccgcagcgc gacctctacc aggcggtgca ccgggcgcag
    22441 aagaaggcga ttgcggcggt gaagcccggc gtgaagttcc gcgacgtgca cctgctcgcc
    22501 tgccgcagcc tggccgagga cctgaagacg atgggcctga tgaaggggaa cctcgacgcc
    22561 gccatcgagc agggggcgca ggggatgttc ttccagtgcg gcctcggcca catgctgggc
    22621 ctcgacgtgc acgacatgga ggggctgggc gagcagcacg tcgggtacga cgcctcggtg
    22681 cagcgcagcg cccagttcgg cctcaagtcg ctgcgcatgg cacgccctct cgaacccggg
    22741 atggtcatga cggtcgagcc gggcatctac atgatcccgg tgctcatggc ccggtggcgg
    22801 gcggaaggga agttcgccga gttcctcgat tacgatgcca tcgaacggtt ccgcgccttc
    22861 ggcggcatcc gcctcgagga cgacgtgctg gtgaccgaga cggggaaccg ggtcctcggc
    22921 cagccgatcc cgatcgagat ggaagaggtg gaagcgctcg cacgccagta gcgccggcct
    22981 ccgtgccggc atgagtcgca ccggcctccg tgccgttcgc gaagggagga gtccgaatgg
    23041 agccgaaaca cgttgtggtc ggcctgcacc tcaccgaccg tttccagcag gcgacccgag
    23101 tccagagtct gctgaccgag tacggccacc agatcaagac ccgcatcggg ctgcaccgga
    23161 ccgaggagct gggcacgccg cagggggtca tcctgctcga gatcgtcggc gacgacgcgg
    23221 gccgcgttgc gctggtcggg aagctgcggg aggtgcccgg cgtggaggtg caggagatcg
    23281 tgttcgagca ctgctgagcg ggccggaaga tcccgcgatc cagtcgtgcc ggaggtgcag
    23341 gaggtgcaga aggtgccgcg ggtgctagtg ctggtggtgc caaaggtgct ggtgccagcg
    23401 gtgcaggcgc ggcacgctcg gcaccagcac cttcggcacc agcacttccg gcaccatcag
    23461 caccagcacc ttcggcacca tcggcaccag caccttcggc acc
//
LOCUS       DAOPQX010000014        22982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2790_length_22982_cov_4.493436, whole genome shotgun sequence.
ACCESSION   DAOPQX010000014 DAOPQX010000000
VERSION     DAOPQX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 22982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22982
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2790_length_22982_cov_4.493436"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..305)
                     /locus_tag="PKK95_01610"
     CDS             complement(84..305)
                     /locus_tag="PKK95_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOC16928.1"
                     /translation="MSQPVRISARNQLKGVVEEIRTEGLLAQVRLRVGSETVTAVITR
                     EAVDELKLAVGQPALAIIKATEVLLAREA"
     gene            350..910
                     /locus_tag="PKK95_01615"
     CDS             350..910
                     /locus_tag="PKK95_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HOC16929.1"
                     /translation="MKGAFLAAVLAAACVPALIAQQSSDGEGQAVARARAAIAGEGIK
                     GTAEFTEVARGAGREVQVVVRVTGLTPGLHGMHLHAVGKCEPPFTSAGGHFDPGPYGH
                     TDPDTNHPFHMGDLPNIEAKGQSEATVRATTTRVTLSDGPLTVFDKDGTALIIHANPD
                     QGITGPEKSGVSGGPRVACGVIERLK"
     gene            complement(1010..2575)
                     /locus_tag="PKK95_01620"
     CDS             complement(1010..2575)
                     /locus_tag="PKK95_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC16930.1"
                     /translation="MARWLPSGPARRAALALLVAAIVTVPLVAQIVQEPVDYDAVYRI
                     KEEGLQRSKAMELMSYATDVYGPRLTASPYRAAFTEWFKKTVGEWGLVNVRTEPIPFG
                     RGWTNERFSAHVVSGQPYPLIGYPKAWTPGTEGPVRGEAVIAIIEDESGFAKFKGQLK
                     GKFVLSMPAREFTTPFEPLARRHNAADLEALEQLGDGGQRRRPPMGDRAFRQKLMAFY
                     LSEGVAAVLDSGRGETGTTMVGSGGSHDPKQPPAPCQVSLAGEHYNRIFRTLEKKVPV
                     TIEMDVRNRFHEKAEAYNILAEIPGTDKADEVVLIGGHWDSWHAGTGAADNAAGFVPM
                     MEAMRILKASGVTLRRTVRLALWDGEEQGLIGSREYVKKYLADRQTMQLEPGHSKLSA
                     YFNIDNGTGAIRGVYLQGNEAVAPIFRAWMEPFRSLGMTTLSIRNTGGTDHLSFDAVG
                     VPGFQFIQDPVQYGRTYHTNMDTYERIEASDLMRNSVIVAAFAYHAANREQLLPRKPL
                     PKPAPAGQGRGMF"
     gene            complement(2645..3325)
                     /locus_tag="PKK95_01625"
     CDS             complement(2645..3325)
                     /locus_tag="PKK95_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOC16931.1"
                     /translation="MSLEAVIFDMDGVLLDSEPVHFEATCALLAGYGITYTAAPNENF
                     FGCTDRDVFRVLKARYGLEPDEDTLTREWIGHVLPRLAERIHPLPGVPEVLFELRRRG
                     VRLALASSSAAPVIAASLEALGLAGVFETRVSGDDVSQGKPSPEIFLQALGRLDLPAS
                     ACLVVEDSYNGLLAAHAAGIPCAVVPCSSTAHQDFTGADVQLSTLQDLVEWVEARRGP
                     GTGAPARR"
     gene            complement(3322..4416)
                     /locus_tag="PKK95_01630"
     CDS             complement(3322..4416)
                     /locus_tag="PKK95_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HOC16932.1"
                     /translation="MLLALRLGVVAAAVVAALLPLPPALVERAYSTGLYPAWQHAATS
                     VSDLVPFALLDVVIVLVAVRALVLLVRVLRPGSRPWLRTAVGSLVRLAALAAVLYLAF
                     LVSWGLNYRRAPLVSRLDHDAARVTPEAASALARRIVAGANRLHGAAHAEQAASWAAV
                     EASLAPPFAAVQAQVLGGTPARPARAKRSALTYYFERAGVDGMTDPFFLEVLVNRQLL
                     PFERPAVLAHEWAHLAGFADEAEANFVGWLVCLQAQPPAQYSAHLAILWDLLNALPPD
                     ERKRAAIGLAPGPRADLEAVAARVARAAPALRQVSWRVYDRYLKANRVEAGVGSYGAA
                     LQLLLGTRMAPGWKPAPRPQARGGAEDATT"
     gene            complement(4435..6852)
                     /locus_tag="PKK95_01635"
     CDS             complement(4435..6852)
                     /locus_tag="PKK95_01635"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOC16933.1"
                     /translation="METTVRRIAGELGIGDDQVETVLRLLADGATPPFLARYRREVTG
                     GLDSARLRAIEERVHQVRELDERRAFVLKSLSEQGRLTPELQTAIATAESRARLEDLY
                     LPFRQKRKSRASQAREAGLEPLADSLLDNHDLVPQQEAERYVDAARGVPDRSAALDGA
                     RWILLARFSEDAPLLEAIRRYVRDHAVLQSRVIEGRQEKGSRFAEFFALSEPVRSISA
                     ARVLGVLRGRKEGVLRVSLALPGPDPAPAAAPPAPSVEPSEAGAPAAAPEAPREPPSV
                     PEQLIAERFGIVHEGRAADAWLWETVRRAWKMKIFPYVQVEVEGHLRERAERESIHSY
                     ARSLRDLLMAAPAGPRPVLGLDPGLRTGVKAAAVDAAGTVLETATVFPHQPRNEWDQA
                     CETLAGLIERHGAELVAIGNGTGSRETDRLLSDIAKRRPELKFSKVIVSEAGVSAYGS
                     SRLAARELPELDAPLRAAVTVARRVQDPLHELAKVEPRTIGVGQYQHDVNQAHLSRAL
                     GAVVEDCTAEVGVDVNAASSALLGRVPGLSHRLADNIVAVRSTVGSFASREDLRRVPG
                     MTERAWEQSAGFLRIVGGPQPLDATRIHPESYAVVEAIAASAGRPVADLVGQPEALDG
                     LSPEQFVDEQHGLPTLTDVFAELRAPGHDPRPAFRLATFQEGLDDLSDLKPGMVLEGV
                     VTNVATFGAFVDIGVHQDGLVHVSRLADRFVKDPHEVVKTGEVVRVKVLEVDLERKRI
                     ALTMRFEKKPATRHVPQRPARDAAPAGGSRGTRRKPPVQVPSPSTRQPAAETAMAAAF
                     SRLLKRS"
     gene            complement(6943..8295)
                     /locus_tag="PKK95_01640"
     CDS             complement(6943..8295)
                     /locus_tag="PKK95_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOC16934.1"
                     /translation="MSSTLLERWFGLSARGTSARTEVLAGVTTFATMAYILVVQPAIL
                     STDLAGRPLGLDFGAVFVATCLGSAFASILMGLYAGYPIALAPGMGENVFFVTVVMGL
                     AALGVAQPWQVALGMVFVSGVAFILLTLVGVREALIDAISPSMRSAIAVGIGLFIAFL
                     GLRQGGLVVGRPTTLVGLNPELRSAEVGVFAAGLLLSSVLLARRVRGALLLGMAGSAL
                     VAALLGKVAFKGIVALPQVAHSAVLAMDPIGALEPRLLPFVIVFLFMGLFDTVGTLVG
                     VSEQAGLLQGGRLPRANRALLTDAAGITVGAALGTSTITCYIESAAGVSAGGRTGLTA
                     VVAGALFLAAVFFGPLVAALGSYGPITAPALVLVGSMMMANAARIAWDDLTEALPVFL
                     TVVGIPLTSSIADGLALGFVAYPVVKLMAGRGREVGWLSYLMAALLLAYFVFVRHQLG
                     "
     gene            8519..8965
                     /locus_tag="PKK95_01645"
     CDS             8519..8965
                     /locus_tag="PKK95_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC16935.1"
                     /translation="MQHRTTTGHAGDTRRHEERRPIVLLVDDYLDCREMYAMYLSLAG
                     FRVVKARDGFEALALAREERPDLVLMDLGLPGIDGCEATRRLKQDRATRDVPVVALTA
                     QAPPTGNLLEAAGFEAVITKPCLPDELAEQAHRFVRRRVAGTHPLA"
     gene            9179..10219
                     /locus_tag="PKK95_01650"
     CDS             9179..10219
                     /locus_tag="PKK95_01650"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC16936.1"
                     /translation="MVHIPIVGFVLLASLVLGGGCKKDTPETAKAASPAPTAAQTAAP
                     APPKPVPAELPDVLARVNGRAVTKAEFQQAVRNLEAQAGGPVPPDRRDLIYRQVLDQL
                     VGFRLLVQESEVRKTPIPETEITSRLAEIRKQFPDEAAFKAALAERGTTPEKLAAEFR
                     EQLAAMKLVETVVTPTVAVGDQQLDEFYKKNPERFQQPEAVRTSHILIRVPEKAEAAA
                     RRAARAEADRVRAAAFKGEDFAALAKQHSQDQGSAVNGGDLGFVVRGQTVPAFEEAAF
                     SLKPGQVSQVVETTFGYHVLKVGEHRAARTVPLDEVKGEVTEFLKQQQLQEKTAAFVE
                     QLKAKAKVEILI"
     gene            complement(10227..11906)
                     /locus_tag="PKK95_01655"
     CDS             complement(10227..11906)
                     /locus_tag="PKK95_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC16937.1"
                     /translation="MRTEASVNADRPLVGGEAIERALRERSAWLDAIFDASRDGIIVE
                     ENERIVFVNRAYVRLYGYDRPEELIGQHVSIVQAPADNERMLDFGRRRLAGEPVPEIY
                     DFTGRRRDGSAIQLEASVAIATIADRHVIVTVVRDVSERRRAETERAKRLVEHALRLE
                     AQEAQKRMEALYREADEASRLKDEFLATLSHELRTPLNAMLGWARLLRGGTLDGATRG
                     RALESIERNTQLLAQLVSDILDVSRIITGKLRLDRGAVDLVPLVEAALESVRPAADAK
                     NLRMALHAEGVIEPLLADGDRLLQVVWNLLANAVKFTPEGGRVDVSVTGRPGSVEVAV
                     QDDGAGIDPSFLPYVFDRFRQADASTTRTHGGLGLGLSIVRHLVELHGGTVEAASDGE
                     GRGARFTVRLPARPAPGGARPTAPAAPPEAAVGAPGGQRALENIRVLVVDDEADARGL
                     MLAALQAEGASVVTSASARQALDVFPDFRPDVLLADIGMAGEDGYSLIRKVRALPDPA
                     IARVQAVAVTAYARGEDRQAAIGAGFDRHLAKPFDPSALVALVAALCRGGR"
     gene            complement(11909..12391)
                     /locus_tag="PKK95_01660"
     CDS             complement(11909..12391)
                     /locus_tag="PKK95_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOC16938.1"
                     /translation="MTLDLVPVSIPPDANVIIGQSHFIKTVEDLYEVMVTSAPRAAFG
                     LAFNEASGPCLIRVEGNDADLQQAAIATASALGAGHVFVLYLRGAFPINVLDRIKACP
                     EVCRVFCATANPLEVIVARSGQGGGVLGVIDGASPGGVEQDADREARRAFLRTIGYKR
                     "
     gene            complement(12435..14468)
                     /locus_tag="PKK95_01665"
     CDS             complement(12435..14468)
                     /locus_tag="PKK95_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOC16939.1"
                     /translation="MALVRRLERSGGRYEVDELLGREWLVTNGLGGYASGSVAGVPTR
                     RYHGVLVAALPGPLGRMVMLNHVGETLVDEEGGRVPLSTIELTGGLAEPEAPACLEEF
                     AVEDGLPVWRFRHHDLVVEKRLLMPHRQNTTHVFYRLAAGGERSTLELRLFVHIRPHE
                     GEVSERLDTSYTLTAFEDQFELSAPDLPALRLTAGEGANFAVESREIADVLYRVEQAR
                     GYPAGGALHSPGFFTLRLERGGTASLTASTESWETIHALLPGDALKAEGRRRGRLVAQ
                     ADPRARGGFARELVYAADQFVIQPAGRAAEAVRAHAEGDAVRTVVAGYPWFTDWGRDT
                     MIALEGLTIVTGRSVQAGYILRTFGRYLRDGLIPNLFPEGQREGLYHTADATLWFFHA
                     IDRYLDATGDRETLRNLLPDLRRSVDWHVQGTHFGIKVDPSDGLLTQGAEGYQLTWMD
                     AKVDDWVVTPRRGKAVEINALWYNALRLLEGWLRQEDGETAATPLAEQAGQARASFNR
                     RFWYEQGRHLYDVVDGEQGDDGSCRPNQVLAIALPHPILDRGRWASVLEAVERELLTP
                     CGLRSLAPGERDYKAQYYGDLRARDAAYHQGTVWAWLIGPFVDAWLKLHPGDRQGARR
                     FLESFPDAIGRFAIGTIAEIFDAEPPFTPRGCVAQAWSVAEVLRAWLKTSPDA"
     gene            complement(14468..16384)
                     /gene="treZ"
                     /locus_tag="PKK95_01670"
     CDS             complement(14468..16384)
                     /gene="treZ"
                     /locus_tag="PKK95_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOC16940.1"
                     /translation="MRNQPGRADGRRLPIGAEVMAAGGVHFRVWAPGHARLDLVLEDG
                     GSGRTLKLSMECEPGGYHSAFAPQAGAGTRYRFRAAGHDRLLQDPASRFQPDGPHGPS
                     EVVDPSDYRWSDAAWPGVKLEGKVLYEMHVGTFTPSGTWEAASRQLDELRAAGVTVIE
                     MMPVADFPGRFGWGYDGVDLFAPTRLYGRPDDLRRFVDRAHAAGLGVILDVVYNHVGP
                     AGSCHKAFAADYFTDRYSNEWGEAINFDGPNAAPVREFYAANARYWIEEFHFDGLRLD
                     AVQAIHDASPCHILAEIAAAARAAAGGRSILLVAENEVQDARTVRPASEGGYGIDAVW
                     NDDYHHSALVALTGRNEAYYTDHLGVAQEFISAAKYGYLYQGQRYAWQRKARGTPTWG
                     LPPASFVTFIENHDQVANSGRGRRVRQLTSPGRYRAVTALTLLGPGTPMLFQGQEFGS
                     SSPFLFFADHDDTLGSAVRAGRAEFLRQFPSLAGEAMQRQLADPGAFETFARCRLDFS
                     ERERHAPIYALHRDLLRLRREDPAFRAQRPGGLDGAVLAPEALVLRFFGPGGHDGDRL
                     LLLNLGRDLTLRVAPEPLLAPPEHLRWEVLWSSEDPRYEGGGTPEVVTPEGLRVPGES
                     AVALAGRPHAEEGD"
     gene            16619..17467
                     /locus_tag="PKK95_01675"
     CDS             16619..17467
                     /locus_tag="PKK95_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOC16941.1"
                     /translation="MAENIRTPNLQYLDAGYVADDRIDFDGLDVRNAAGDKLGSVDGF
                     IVQRNSNRPYYLVVDSGGWFSSRHYLVPIGHVRLDPDNRALRVDIERDTIQGFPEVHI
                     DRFDEFTEEEVRRIDERTLTALAPNEVAARMGDTWDYDRWSHYSQPDWWRAQPADLPP
                     PPLTNRPLAEDYAPDVAGTDAEVTGYAPEHITARHDEDLARRTDRRDLEGLGTIRRSD
                     TAAGTPPEDLNDRAQPGDVLGIETAGETTSLGDTARDEDKRREEAEEANREIFENQRR
                     DRRRDG"
     gene            complement(17527..18900)
                     /locus_tag="PKK95_01680"
     CDS             complement(17527..18900)
                     /locus_tag="PKK95_01680"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOC16942.1"
                     /translation="MNFRTIIEPFRIKSVEAIAFTTREERAEALRAAGYNVFRLPAAE
                     VLIDLLTDSGTGAMSAAQWGALMQGDESYAGSRSFYRFEAAVRDLTGFKHVIPTHQGR
                     AAEHILFHTVLRPGQIVPSNNHFDTTRANIEAEACEARDLVISEGRVPALVHPFKGNV
                     DLAALERTLAEAGDRVPLVMATITNNSGGGQPVSLENLRGIRALCDRYRKPFFLDACR
                     FAENAYFVKVREAGQGGRTPKAIAQELFSLADGCTMSAKKDGLANIGGFLALNDDRWA
                     EEARNLLILTEGFPTYGGLAGYDLEAIARGLEEVVEEPYLRYRIRSTEYLGDKLTAAL
                     VPTIRPAGGHAIYLDAKALLPHVAPLEYPGVALANALYVEAGIRGVEIGTLMFGRKPD
                     GSEVAAPMELVRLAIPRRVYTQSHIDYVAEGVIEVARKKDSIRGYRIASSPAVLRHFT
                     ATFEPLS"
     gene            complement(18902..20014)
                     /gene="metK"
                     /locus_tag="PKK95_01685"
     CDS             complement(18902..20014)
                     /gene="metK"
                     /locus_tag="PKK95_01685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOC16943.1"
                     /translation="MEKLTHTLTSESVSEGHPDKVCDYIADSILDAHLSGDARSRVAC
                     EVLCKDGQVVLAGEITSRTPVDYEGIARAAIREIGYVFDGEAFHADRVKVLQFISRQS
                     DDIAQGVDEGRGLDVEQGAGDQGMMFGYATDETPELMPLPIVLAHRLTRGLAEDRRSG
                     KYPWIRPDSKSQVSVEYANGRPARVTTVLVSTQHDQDTPHAEIERYVATCLAPRVLDS
                     WFTPEVKVLVNPTGSFALGGPSADCGVTGRKIIVDTYGGAAHHGGGAFSGKDPSKVDR
                     SGAYFARYVARQVVKAGIARRAEVQVAYAIGKAQPVSTKVETFGTGDEKAAREMVDRF
                     DFRPAAIIESLDLLRPIYRATTNYGHFGKTGLPWEA"
     gene            22180..22884
                     /locus_tag="PKK95_01690"
     CDS             22180..22884
                     /locus_tag="PKK95_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC16944.1"
                     /translation="MLDTLPERFALARQQQLAHQDFLLVVLGDEASRRDALSVTTRVQ
                     RAHLDPTMQLEAWDPTAKVTFDKTLLNDLASLRFLDAHAHVAIVGPVGVGKTFLAHAL
                     GYAACRRGRSVLALRADRMLKTLKHARLDHTYEAELRKLLAVDLLILDDFALDTMDPT
                     ESRDIYELLTERHRVGSIIVTSNRAPDEWLATFADPVRAQSAIDRFTSNAYDLLIEGE
                     SYRSRQKPGRRSGAAA"
BASE COUNT         3484 a         8274 c         7983 g         3241 t
ORIGIN      
        1 gggaatcgtc ctgtcggacg agacagcgat cggccgcgac ccgatccgcg cggtccggac
       61 cgcctcgacc ctcctgcagg ccctcacgcc tcccgcgcca gcagcacctc ggtagccttg
      121 atgatggcca gcgccggctg accgacggcc agcttcaact cgtccaccgc ttcacgggtg
      181 atgacggcgg tgacggtttc gctgccgacc cgcaagcgga cctgcgccag caggccctcg
      241 gtgcggatct cctcgacgac acccttcagt tggtttcgcg cgctgattcg cacgggctgg
      301 ctcatgaagc gattgtaccg tgtatcatcg aggtggaggg cacctgccta tgaagggagc
      361 gtttctggcg gccgtgctgg cggccgcgtg cgtgccggca ctgatcgcgc aacagtcgtc
      421 ggacggcgag ggccaggcgg tcgccagggc ccgagccgcg atcgcgggag agggcatcaa
      481 ggggaccgcg gaattcaccg aggtcgcgcg gggcgccggg cgcgaggtgc aggtggtggt
      541 cagggtgacg gggctgacgc cgggcctgca cggcatgcac ctgcacgcgg tcgggaagtg
      601 cgagccgccg ttcacctcgg cgggcggtca cttcgacccc ggtccctacg ggcacaccga
      661 ccccgacacc aaccacccgt tccacatggg cgacctgccc aacatcgagg cgaagggcca
      721 gagcgaggcg acggtgaggg cgaccaccac ccgcgtcacc ttgtcggacg ggccgctgac
      781 ggtgttcgac aaggacggca ccgcgctcat catccatgcg aaccccgacc agggaatcac
      841 ggggcccgag aagtcgggcg tcagcggcgg cccgcgcgtc gcgtgcggag tgatcgagag
      901 gttgaagtag ggacgaattc ggtcggcggc gggcgcggga cgggagaaat ggagaaagca
      961 gaagggagca cttccccccc tcctgctttt cacattcagc tttaccgccc tagaacatcc
     1021 cccgcccctg ccccgccggc gccggcttgg gcagcggctt tcgtggcagc agctgctcgc
     1081 ggttggcagc gtggtacgcg aaggcggcga cgatcaccga gttccgcatc aggtcgctcg
     1141 cctcgatccg ctcgtaggtg tccatgttcg tgtggtacgt ccggccgtac tggaccgggt
     1201 cctggatgaa ctggaagccg ggcacgccca ccgcgtcgaa cgacaggtga tcggtcccgc
     1261 ccgtgttgcg gatcgacagc gtggtcatcc cgaggctgcg gaagggctcc atccaggccc
     1321 ggaagatcgg cgcgactgcc tcgttgccct gcaagtacac gccgcggatg gcgccggtgc
     1381 cgttgtcgat attgaagtag gccgagagct tcgagtgtcc cggctcgagc tgcatcgtct
     1441 ggcggtcggc caggtacttc ttgacgtact cgcgcgagcc gatcagcccc tgctcctccc
     1501 catcccacag cgcgaggcga acggtgcgcc gcagcgtgac gccgctcgcc ttgaggatcc
     1561 gcatcgcctc catcatcggg acgaagccgg ccgcgttgtc cgcggcgccg gtgccggcgt
     1621 gccacgagtc ccagtgcccg ccgatgagca cgacttcgtc ggccttgtcg gtccccggga
     1681 tctcggccag gatgttgtag gcctccgcct tttcgtggaa gcggttccgc acgtccatct
     1741 cgatggtcac cggcaccttc ttctcgagcg ttcggaagat ccggttgtag tgctcgccgg
     1801 cgagcgacac ctggcacggc gcgggaggct gcttcgggtc gtgcgacccg ccgcttccca
     1861 ccatcgtggt tcccgtctcg ccgcggcccg agtcgagcac cgccgcgacc ccttccgaca
     1921 ggtagaaggc catcagcttc tggcggaagg cgcgatcgcc catgggcgga cgccgccgct
     1981 ggccgccgtc gcccagctgc tcgagcgcct cgaggtcggc ggcgttgtgc cggcgagcga
     2041 ggggctcgaa gggcgtggtg aactcgcgcg ccggcatcga caggacgaac ttccctttca
     2101 gctgcccctt gaacttcgcg aaccccgatt cgtcctcgat gatcgcgatc actgcctcgc
     2161 cgcgcaccgg cccctcggtc cctggcgtcc aggccttggg gtacccgatg agcgggtagg
     2221 gctggcccga cacgacgtga gcgctgaagc gctcgttcgt ccacccgcgg ccgaacggga
     2281 tcggctcggt gcgaacgttg acgagccccc actcccccac ggtcttcttg aaccactcgg
     2341 tgaaggcggc gcggtagggc gaagcggtca gcctcggccc gtacacgtcg gtggcatagc
     2401 tcatcagctc catggccttg gatcgctgga gcccttcttc cttgatgcgg tagacggcat
     2461 cgtagtcgac cggttcctgc acgatctgcg cgacgagcgg gacggtgacg atcgcggcga
     2521 ccagcagcgc gagggcggcg cggcgcgccg gcccggaagg tagccatcga gccatgagcg
     2581 aatctctcct gtcggggact tcgaagacaa tttcagcgca atacgaccgg ggggctcgaa
     2641 tggtttaccg gcgtgccggc gcgccggtgc cggggcctcg cctcgcctcg acccactcga
     2701 ccaggtcctg cagggtcgag agctggacgt cggcgccggt gaaatcctgg tgggcggtgg
     2761 aggagcaggg cacgaccgcg cagggaatgc cggcggcgtg tgcggccagc aggccgttgt
     2821 acgagtcctc gaccaccagg caggccgagg ccggcaggtc gaggcgcccg agagcctgga
     2881 ggaagatctc gggcgagggt ttcccctgcg agacgtcgtc gcccgacacg cgggtctcga
     2941 agacgccggc caggccgagc gcctcgaggc tggccgcgat gaccggggcg gccgacgagg
     3001 aggccagggc caggcgcacg ccgcgccggc gcagctcgaa caacacctcg gggacgcctg
     3061 gcagggggtg gatgcgttcg gccaggcgcg gcagcacgtg gccgatccac tcgcgggtga
     3121 gcgtgtcctc gtccggctcg aggccgtagc gggccttcag cacgcggaag acgtcgcggt
     3181 ccgtgcagcc aaagaagttc tcgttcgggg cggccgtgta ggtgatgccg tacccggcga
     3241 gcagcgcgca ggtcgcttcg aagtgcaccg gctccgagtc gagcagcaca ccgtccatgt
     3301 cgaagatcac cgcctcaaga ctcatgtcgt cgcgtcctcc gccccgccgc gagcctgcgg
     3361 ccgcggtgcg ggcttccatc ccggcgccat ccgggttccc aacagcaact gcagcgccgc
     3421 gccgtagctg ccgaccccgg cctcgacgcg gttcgccttc aggtatcgat cgtacacccg
     3481 ccagctgacc tgccgcagcg cgggggcggc cctggcgacg cgggcggcca cggcctcgag
     3541 atccgcgcgg ggcccgggtg ccagcccgat cgccgcgcgc ttgcgctcgt cgggcggcag
     3601 ggcgttgagc aggtcccaga ggatcgcgag gtgcgcgctg tactgcgcgg gcggctgcgc
     3661 ctgcaggcac accagccagc cgacgaagtt cgcctcggcc tcgtcggcga agcccgccag
     3721 gtgcgcccac tcgtgcgcca gcactgccgg ccgctcgaag ggaaggagct ggcggttgac
     3781 gagcacctcg aggaagaagg ggtcggtcat gccgtccacc ccggcccgct cgaagtagta
     3841 cgtcagggcc gaccgcttcg cccgggccgg gcgggcgggc gttccaccga gcacctgtgc
     3901 ctgcaccgcc gcgaacggcg gcgcgaggct ggcctcgacg gccgcccagg acgccgcctg
     3961 ctcggcatgc gccgcgccgt gcaggcggtt ggcgcccgcg acgatgcgcc gcgcgagcgc
     4021 cgaggccgcc tccggggtga cccgggcagc gtcgtgatcg aggcgcgaaa ccagcggggc
     4081 ccgccggtaa ttcaaccccc acgagacgag gaacgccagg tacaagactg cggccagcgc
     4141 cgccaggcgg acgaggctgc cgacggccgt gcgcagccag gggcgcgacc cgggccgcag
     4201 tacccgcacc agcaggacga gcgcccgaac cgcgacgagc acaatcacga cgtcgagcag
     4261 cgcaaagggc acgaggtcgg acacggaggt cgccgcgtgc tgccaggcgg gatagaggcc
     4321 ggtggagtac gcacgctcca ccagcgcggg cggcagcggg agcagtgccg cgaccacggc
     4381 ggccgccacg acgccgaggc gcagggcgag cagcatcgaa ggcggccccc ggcctcagga
     4441 ccgcttcagc aggcgcgaga aggcggcggc catcgccgtc tcggcggccg gctgacgcgt
     4501 cgagggagac ggcacctgca ccgggggctt gcggcgcgtc ccccgcgacc ctcccgcggg
     4561 ggccgcatcg cgggcgggcc gctgcgggac gtggcgcgtg gccggcttct tctcgaagcg
     4621 catggtcagc gcgatccgct tccgctcgag gtcgacctcg agcactttca cgcgaaccac
     4681 ctcgccggtc ttcacgacct cgtgcggatc cttgacgaag cgatccgcga ggcgcgacac
     4741 gtggaccagg ccgtcctggt gcaccccgat gtccacgaag gccccgaagg tcgcgacgtt
     4801 ggtcaccact ccctcgagca ccatccccgg cttcaggtcg gacaggtcgt cgagcccttc
     4861 ctggaacgtc gcgaggcgga acgcgggccg ggggtcgtgc cccggcgccc gcagttcggc
     4921 gaagacgtcg gtgagcgtgg gcagcccgtg ctgctcgtcg acgaactgct cgggcgacag
     4981 cccgtcgagc gcctcgggtt ggccgaccag gtcggccacg gggcggccgg cgctcgcggc
     5041 aatcgcctcg acgaccgcgt aggactcggg gtggatgcgc gtcgcgtcga gcggctgcgg
     5101 gccgccgacg atgcgcagga agccggccga ctgctcccag gcgcgctcgg tcatccctgg
     5161 cactcggcgg aggtcttcac gggatgcgaa ggagccgacc gtgctccgca cggccacgat
     5221 gttgtcggcg agccggtggc tcaagccagg gacgcgcccg agcagggcgg aggacgcggc
     5281 gttgacgtcc acgccgacct cggcggtgca gtcctccacc acggctccca gcgccctcga
     5341 caggtgggcc tggtttacgt cgtgctggta ctgcccgacc ccgatcgtgc gcggctcgac
     5401 cttcgccagc tcgtgcagcg ggtcctgcac ccggcgcgcg accgtgactg ccgcgcgcag
     5461 cggggcgtcc agctcgggca gctcgcgcgc cgcgaggcgt gacgagccgt acgccgagac
     5521 gcccgcctct gacacgatca ccttgctgaa cttgagctcc gggcgccgct tcgcgatgtc
     5581 cgagagcagc cgatcggtct cgcgcgaccc ggtgccgttg ccgatcgcca ccaactcggc
     5641 gccgtgtcgc tcgatcaggc cggccagcgt ctcgcacgcc tggtcccact cgttgcgagg
     5701 ctgatggggg aacacggtcg ctgtctcgag caccgtgccg gcggcgtcaa ccgcggccgc
     5761 cttcacgccg gtccgcaggc cggggtcgag gccgagaacg gggcgcgggc cggcgggcgc
     5821 ggccatcagc agatcgcgaa gggagcgcgc gtacgagtgg atggactcgc gctcggcgcg
     5881 ctcgcgcagg tgcccttcga cctccacctg cacgtacggg aagatcttca tcttccacgc
     5941 ccggcgcacc gtctcccaca gccacgcgtc ggcggcccgc ccctcgtgga caatgccgaa
     6001 gcgctcggcg atcagctgct cggggacgct cggcggctcg cgcggcgctt cgggcgcagc
     6061 ggcgggcgcc cccgcctcgg aaggctcgac ggaaggggcc ggtggcgccg ccgcgggagc
     6121 ggggtctgga ccgggcagcg cgagcgagac ccgcagcacc ccctccttgc ggccgcgcag
     6181 cacgcccagc acccgcgcgg cggagatcga gcggacgggc tcggacaggg cgaagaactc
     6241 cgcgaaccgc gagcccttct cctggcggcc ctcgatcacc cgcgactgca gcaccgcgtg
     6301 gtcgcggacg tagcggcgga tcgcctcgag cagcggtgcg tcctcggaaa agcgcgcgag
     6361 caggatccag cgggccccgt cgagcgccgc cgagcggtcg ggcaccccgc gcgccgcgtc
     6421 cacgtagcgc tccgcctcct gctgcggcac gaggtcgtgg ttgtcgagca gcgagtcggc
     6481 gagcggctcg agcccggcct cgcgtgcctg cgacgcgcgg ctcttgcgct tctgcctgaa
     6541 cgggaggtag aggtcctcga ggcgcgcgcg gctctcggcg gtggcgatcg ccgtctgcag
     6601 ttccggcgtg agccggccct gctcgctgag gctcttcaag acgaaggcgc ggcgctcgtc
     6661 gagctcgcgc acctggtgca cccgctcctc gatcgcgcgc agccgcgcgg agtcgagccc
     6721 gcccgtgacc tcccggcggt atcgcgcgag gaaaggcggc gtggcgccgt cggcgagcag
     6781 ccgcagcacc gtctccacct ggtcgtcccc tatcccgagt tccccggcga tccgccgaac
     6841 ggtcgtctcc ataaacctca ggcccctggc cagcgaccta ccatcgtagc aaacttcgct
     6901 gagccgattc ccgtttttca accgagccgg tgagcacgaa tttcaaccga gctggtggcg
     6961 cacgaagacg aagtacgcga gcagcagcgc ggccatgagg tacgagagcc acccaacctc
     7021 gcgcccccgc cccgccatca gcttcacgac cgggtacgcc acgaagccga gcgcaagccc
     7081 gtcggcgatc gacgaggtga gcgggatgcc gaccaccgtc aggaacaccg gcagcgcctc
     7141 ggtgagatcg tcccacgcga tgcgcgccgc gttcgccatc atcatcgacc ccacgagcac
     7201 cagggcggga gcagtgattg gcccgtacga cccgagcgcc gccaccagcg ggccgaagaa
     7261 gaccgccgcc aggaagaggg cccccgcgac caccgccgtc agcccggtcc gcccgcccgc
     7321 cgacacgccg gccgcgctct cgatgtagca ggtgatcgtg ctcgtgccga gcgctgcgcc
     7381 aaccgtgatc cccgcggcgt cggtgagcag cgcccggttc gcccgcggca gccgcccccc
     7441 ttggagcagc cccgcctgct cggagacacc gaccagtgtt ccaaccgtgt cgaacagccc
     7501 catgaacagg aacacgatca cgaagggcag gagccgcggc tcgagcgcgc cgatcggatc
     7561 catggccagc accgccgagt gcgccacctg cgggagcgcg acgatcccct tgaacgccac
     7621 cttccccagc agcgccgcga ccagcgccga cccggccatt cccaagagca gggcgccgcg
     7681 cacccggcga gccagcagga ccgacgagag cagcaggccg gccgcgaaca cgccgacctc
     7741 cgccgagcgc agctccgggt tgaggccgac cagcgtcgtc ggccgcccca ccacgaggcc
     7801 gccttgcctg agccccaaga aggcgatgaa cagcccgatc ccgaccgcga tcgcgctccg
     7861 catgctgggg ctgatggcgt cgatgagcgc ttcgcgcacg cccaccagcg tcagcaggat
     7921 gaaggccacg cccgacacga agaccatccc gagcgccacc tgccacggct gggcgacgcc
     7981 aagcgcggcc agccccatga ccaccgtgac gaagaagacg ttctcaccca tgccgggcgc
     8041 cagcgcgatc gggtagcccg cgtagagacc catcaggatg gacgcgaagg ccgagccgag
     8101 gcaggtcgct acgaacaccg cgccgaagtc gaggcccaga ggccggcctg ccaggtcggt
     8161 cgacaggatc gcgggctgaa ccaccaggat gtaagccatc gtggcgaagg tggtcacgcc
     8221 ggcgagcacc tcggtccggg cactggtccc gcgtgcggag aggccgaacc accgctcgag
     8281 gagcgtcgag gacatggcgc cccggcccgc cgcttccctg gcggtcccgg tcaaggacgg
     8341 gctggcactg tggaaatccc cgtctgcacc cgaaaccatg tacgagcacc tccattggcg
     8401 cgaggcacga ggccacgcgg cggcaacttg tggggcggca gcggtcttgc attgagagcg
     8461 accgaaggag aatgcagctc gtctgctgac gcgccacgtg acggattcgg caacgcttgt
     8521 gcagcatcga actacaaccg ggcacgcggg tgacacgcgt cggcacgagg agcggcgccc
     8581 catcgtcctg ctcgtggatg actacctcga ctgccgagaa atgtacgcga tgtatctgag
     8641 cctcgcgggc ttccgggtcg tcaaggcccg cgacgggttc gaggccctcg cgctggctcg
     8701 cgaggaacgc ccggatctgg tgctgatgga cctggggctg cccgggatcg acggctgcga
     8761 agccacgcgg cggctcaagc aagaccgcgc tacccgcgac gtgccggtgg tcgccctcac
     8821 ggcccaggcg ccgccgaccg ggaacctgct cgaggcagct ggtttcgagg cggtgattac
     8881 caagccctgc ctgcccgacg agctcgccga gcaggcgcac cgcttcgtcc ggcgtcgcgt
     8941 cgccggcacg cacccgctcg cctagggccg ggtttttccc ttcccacccg cgcggccctc
     9001 tgctaccctg aacgcgagtg ttcattcccc tgccagctct ctccgcgcca agagccgatc
     9061 gaacccgccc gttgattcgc tccctcgcag cctgagcgga agcagtcaga gtgagcctca
     9121 tcatcgcacg tgtcaacagg aaggtcacgc cgggcgggcg agaacagccg tgaagaccat
     9181 ggtccacatc ccgatcgtcg gcttcgtcct tctcgccagc ctcgtgctcg gcggagggtg
     9241 caagaaagac actccggaga cggcgaaggc cgcctccccg gcgccaacgg ccgcgcagac
     9301 cgccgcaccc gcgccgccca agccggttcc cgccgagttg cctgacgtcc tcgcgcgagt
     9361 gaacggccgg gcggtgacca aggcggagtt ccagcaggcc gtgcggaacc tcgaggcaca
     9421 ggccggcggg cccgtgccgc ccgatcgccg cgacctcatc taccgccagg tcctcgatca
     9481 gctcgtcggc ttccgcctgc tggtgcagga gtcggaggtg cggaagaccc cgatccccga
     9541 gaccgagatc acctcgcgcc tggccgagat ccggaaacag ttccccgacg aggcggcctt
     9601 caaggcggcc ctcgccgagc gcgggacgac gccggagaag ctggccgcgg agttccgcga
     9661 acagctggcg gcgatgaagc tggtcgagac ggtggtgacc ccgactgtcg cggtgggcga
     9721 ccagcagctc gacgagttct acaagaagaa ccccgagcgc ttccagcagc ccgaggcggt
     9781 ccgcacgtcg cacatcctga ttcgcgtgcc cgagaaggcc gaggcggccg cgcggcgcgc
     9841 cgcccgcgcc gaggccgacc gcgttcgcgc cgcggcgttc aagggcgaag acttcgccgc
     9901 gctggccaag cagcactccc aggaccaggg gagcgccgtc aacggcggcg acctcggctt
     9961 cgtcgtgcgc ggccagaccg tcccggcgtt cgaggaggcc gccttcagcc tcaagcccgg
    10021 ccaggtgagc caggtcgtcg agaccacctt cggctaccac gtcctcaagg tcggcgagca
    10081 ccgcgcggcg aggacggttc ccctcgacga ggtgaagggc gaggtgaccg agttcctcaa
    10141 gcagcagcag ctgcaggaga agaccgcggc cttcgtcgag cagctgaagg cgaaagcgaa
    10201 ggtcgagatc ctgatctgac cgcgtcctac cttccgcccc tgcacagcgc cgcgacgagc
    10261 gcgacgaggg cggacggatc gaacggtttc gccaggtggc ggtcgaaccc ggcaccgatt
    10321 gccgcctggc gatcctcacc gcgggcgtag gccgtcaccg ccaccgcctg cactcgcgcg
    10381 atcgccggat cgggcagcgc cctcaccttc cgaatcaggg aatagccatc ctcgccggcc
    10441 atcccgatgt cggccagcag gacgtcgggg cggaagtcgg ggaagacgtc cagggcctgc
    10501 cgcgccgagg ccgacgtcac gaccgaggcc ccctcggcct ggagggccgc cagcatcagc
    10561 ccgcgcgcat cggcctcgtc gtccaccacc agcacccgga tgttctccag ggcccgctgc
    10621 cctcccggcg cgccgaccgc cgcctccggc ggcgccgcgg gggccgtggg acgcgccccc
    10681 ccgggcgcgg ggcgagccgg cagccgcacg gtgaagcggg cgccgcggcc ctccccgtcg
    10741 ctcgccgcct cgacggttcc cccgtgcagt tccaccaggt gcctgacgat cgagaggccg
    10801 aggcccagcc ctccatgcgt gcgggtggtg gaggcatcgg cctgccggaa ccggtcgaac
    10861 acgtaaggca ggaagctggg atcaatgccg gccccatcgt cctgcacggc gacttccacg
    10921 ctgccgggtc gtcccgtcac cgagacgtcc acgcggccac cctcgggcgt gaacttcacg
    10981 gcgttggcca gcaggttcca cacgacctgc agcagccggt cgccgtcggc cagcagcggc
    11041 tcgatcacgc cctccgcgtg cagcgccatc cgcaggttct tggcgtcggc ggcaggccgc
    11101 accgactcga gtgccgcctc gaccaagggc acgagatcga cggcgcctcg gtcgagcctg
    11161 agcttccccg tgatgatccg ggatacgtcg aggatgtcgg agacgagctg ggcgagcagt
    11221 tgcgtgttgc gctcgatcga ctcgagcgca cggccgcgcg tggccccgtc gagcgtgccg
    11281 ccgcggagca ggcgcgccca tccgagcatc gcgttcagcg gcgttcgaag ttcgtgcgac
    11341 agggtcgcga ggaactcgtc cttgagccgg ctcgcctcgt cggcctcgcg gtagagcgcc
    11401 tccatgcgct tctgcgcctc ctgggcctcg aggcggagcg cgtgctcgac gaggcgcttg
    11461 gcgcgctcgg tctcggcgcg gcgcctctcc gagacgtcgc ggacgacggt gacgatgacg
    11521 tggcgatcgg cgatggtcgc gatcgctacc gacgcctcga gctggattgc cgagccgtcg
    11581 cgccggcgtc ccgtgaagtc gtagatctcc gggaccggct cccccgccag gcgacgccgc
    11641 ccgaagtcga gcatccgctc gttgtcggcc ggcgcctgca cgatcgacac gtgctggccg
    11701 atcagctcct cggggcggtc gtacccgtag agccgcacgt aggcgcggtt cacgaagacg
    11761 atccgctcgt tctcctcgac gatgatgccg tctcgcgagg cgtcgaagat ggcgtcgagc
    11821 caggcgctcc gctcgcgaag cgcgcgctcg atcgcctcgc cgcccacgag cggccggtcg
    11881 gcgttcacgg aagcctctgt gcgcaccgct accgcttgta accgatcgtg cgcaggaacg
    11941 cccgcctcgc ctcccggtcc gcgtcctgct cgacgccgcc aggcgacgcg ccgtcgatca
    12001 cccccaggac gccgcccccc tgcccggagc gggcgacgat cacctcgagc gggttggcgg
    12061 tggcgcagaa gacccggcag acctcggggc acgccttgat ccgatcgagc acgttgatgg
    12121 ggaacgcccc gcgcaggtag agcacgaaca cgtgcccggc gccgagcgcc gacgcggtcg
    12181 cgatcgccgc ctgctggagg tcggcgtcgt tgccctccac gcggatcagg caggggcccg
    12241 acgcctcgtt gaaggcgaga ccgaaggccg cgcgtggggc ggaggtgacc atcacctcgt
    12301 agagatcctc gaccgtcttg atgaagtgcg actgtccgat gatgacgttg gcgtcgggcg
    12361 ggatcgacac gggaaccagg tcgagcgtca tggggcgcct ccagagctgg gggggccacc
    12421 gcattgtagc gcgatcacgc gtcgggcgac gtcttcagcc aggcccgcag cacctcggcg
    12481 acgctccacg cctgcgccac gcatccgcgg ggggtgaacg ggggctcggc gtcgaagatc
    12541 tccgcgatcg tcccgatcgc gaaccgcccg atcgcgtcgg gaaagctctc caggaaccgc
    12601 cgcgcgccct ggcgatcacc cgggtgcagc ttcagccagg cgtcgacgaa cgggccgatg
    12661 agccacgccc acacggtgcc ctggtggtac gccgcgtccc tcgcccgcag gtcgccgtag
    12721 tactgcgcct tgtagtcgcg ctcccctggc gccagcgacc gcaacccgca gggcgtcagc
    12781 agctcccgct cgaccgcctc gagcacgctc gcccacctgc cccggtcgag tatcgggtgc
    12841 ggcagcgcga tggccagcac ctggttcggg cggcacgacc cgtcgtcccc ctgttcgccg
    12901 tccacgacgt cgtacaggtg gcggccctgc tcgtaccaga agcggcggtt gaaggacgcg
    12961 cgtgcctgcc cggcctgctc ggcgagcggc gtcgccgccg tctcgccgtc ctcctggcgc
    13021 agccatccct cgagcagccg cagcgcgttg taccagaggg cgttgatctc gaccgccttg
    13081 ccgcgccgcg gcgtcacgac ccagtcgtcc accttggcat ccatccaggt cagttggtag
    13141 ccctcggctc cctgcgtgag caacccgtcg gacgggtcca ccttgatgcc gaagtgagtc
    13201 ccctgcacgt gccagtcgac cgagcgccgc aggtcgggca ggagattgcg cagcgtctcg
    13261 cgatcgccgg tagcgtcgag gtaccggtcg atggcgtgaa agaaccacag cgtcgcatcg
    13321 gccgtgtgat agagcccctc acgctgcccc tcgggaaaca ggttcgggat caggccatcg
    13381 cgcaggtagc gtccgaatgt ccggaggatg tagccggcct gcaccgagcg gccggtgacg
    13441 atcgtgagcc cctcgagcgc gatcatcgtg tcgcgccccc agtcggtgaa ccaggggtag
    13501 ccggcgacca ccgtgcgaac cgcgtcgccc tcggcgtggg ccctcaccgc ttccgcggcg
    13561 cggccggcag gctggatcac gaactggtcg gcggcataca cgagctcgcg cgcgaagccg
    13621 ccccgtgcgc gcgggtcggc ctgcgccacg agccgtcccc gccgccgtcc ctcggctttc
    13681 agcgcgtcgc ccgggagcag cgcgtggatc gtctcccagg actcggtcga tgcggtgagc
    13741 gacgccgtgc ctccgcgctc gaggcgaagc gtgaagaagc ccgggctgtg cagcgcgccg
    13801 cccgccgggt acccgcgcgc ctgctcgacg cggtacagaa cgtcggctat ctcgcgcgac
    13861 tcgaccgcga agttcgcccc ttccccggca gtcagccgca gcgccggcag gtccggcgcc
    13921 gacagctcga actgatcctc gaacgcggtg agcgtgtagg aggtatcgag gcgctccgac
    13981 acctctccct cgtgcgggcg gatgtgcacg aagagccgga gctcgagcgt ggaacgctcg
    14041 cctcccgccg cgaggcggta gaagacgtgc gtggtgttct ggcgatgcgg catcagcagc
    14101 cgcttctcca ccaccaggtc atggtgcctg aagcgccaga cgggcaagcc gtcctccacc
    14161 gcgaactcct cgaggcaggc cggggcctcg ggttcggcca ggccgcctgt cagctcgatc
    14221 gtggacagcg gcacgcggcc gccttcctcg tccaccagcg tctcgcccac gtggttgagc
    14281 atgaccatcc ggccgagggg ccccggcagc gcggcaacga ggacgccgtg gtagcggcgc
    14341 gtcggcacac ccgccaccga accgctcgcg tagccgccca acccgttggt gacgagccac
    14401 tcgcggccga gcagctcgtc cacctcgtac cgcccgccgg accgctcgag acgcctgacg
    14461 agcgccatca gtccccttcc tccgcgtgcg gccgcccggc gagcgcgacg gccgactcgc
    14521 cgggaacccg aagtccctcg ggagtcacga cctcgggagt cccgccgccc tcgtacctcg
    14581 gatcctcgct cgaccagaga acctcccacc ggaggtgctc gggcggggcg agcagcggct
    14641 cgggggcgac ccgcaaggtc aggtcgcggc ccaggttgag gagcagcaac cgatcgccgt
    14701 cgtgcccgcc ggggccgaag aagcgaagca cgagagcctc gggcgcgagc accgccccgt
    14761 caaggcctcc cgggcgctgc gcgcggaagg cgggatcttc gcgccgcagc cgcagcagat
    14821 cgcggtggag cgcgtagatg ggtgcgtggc gctcgcgctc ggagaagtcg aggcggcatc
    14881 gcgcgaaggt ctcgaacgcg cccgggtcgg ccagttggcg ctgcatcgcc tcgcccgcaa
    14941 gcgatgggaa ctgtcgcagg aactcagccc gacctgcgcg gaccgcgctg cccagggtgt
    15001 cgtcgtggtc ggcgaagaag aggaatgggc tcgaggaacc gaactcctgg ccctgaaaga
    15061 gcatcggggt gcccggcccg agcagcgtca gggcggtcac ggcccggtac cggcccgggc
    15121 tggtgagctg ccgcacgcgg cgcccgcgac ccgagttggc cacctgatcg tggttctcga
    15181 tgaaggtgac gaaagaggcg ggcggaaggc cccacgtggg ggtgccgcga gcctttcgct
    15241 gccaggcgta tcgctgcccc tggtagaggt agccgtactt cgccgccgag atgaactcct
    15301 gtgccacgcc gaggtggtcg gtgtaatacg cctcgttccg gccggtcagg gcgacaagcg
    15361 cgctgtggtg atagtcgtcg ttccacaccg cgtcgatgcc gtatcccccc tcggatgcag
    15421 gccgtacggt acgcgcgtcc tgcacctcgt tctccgccac gagcagaatg gacctgccgc
    15481 cggccgccgc gcgagccgcc gctgcgattt ccgcgaggat gtggcacggc gaggcgtcgt
    15541 ggatcgcctg taccgcgtcg aggcgcaggc cgtcgaaatg gaactcctct atccagtacc
    15601 tggcgttggc cgcgtagaac tcgcgcaccg gcgcggcgtt gggtccgtcg aagttgatcg
    15661 cctcgcccca ctcgttcgag tagcggtcgg tgaagtagtc cgcggcgaag gccttgtggc
    15721 acgagccggc cgggcccaca tggttgtaga cgacgtcgag gatgacgccg aggccggctg
    15781 catgtgcgcg gtccacgaac cgccgcaggt cgtcgggcct cccgtagagc cgggtgggag
    15841 cgaacaggtc caccccgtcg tagccccagc cgaagcgccc ggggaagtcg gcgaccggca
    15901 tcatctcgat gaccgtgacg ccggccgcgc gcagctcgtc gagctgccgc gatgcggcct
    15961 cccatgtgcc cgagggggtg aaggtgccca cgtgcatctc gtagaggacc ttcccctcga
    16021 gcttcacacc cggccacgcc gcgtcgctcc agcggtagtc ggacgggtcg acgacctcgg
    16081 aggggccgtg cggtccgtcg ggctggaagc gcgaggccgg gtcctggagc aggcgatcgt
    16141 gccccgccgc gcggaaacgg taccgggtgc cggcgccggc ctgcggcgcg aaggccgaat
    16201 ggtatccacc cggctcgcac tccatggaca gcttgagcgt gcgtccgctc ccaccgtcct
    16261 ccagcacgag gtcgaggcgt gcgtgccccg gcgcccatac gcggaagtgc acgccgcccg
    16321 cggccatcac ctcggcaccg atcggtaagc ggcggccgtc cgcgcgccct ggctggtttc
    16381 tcacgccctc cccttccagc agagttccca ccgctcgtgt gcaggaagga tgcccggccc
    16441 cgctccatgc gatggtccgc gacgggcggg gccgacctgc gttgccggca tcaccggcct
    16501 ggtcacgcca ccttgccggg caccttgctt gcagccccgg tggcagtggc acaggcgtcc
    16561 cgcctgtgag tggtggcagt ggcacaggcg tcccgcctgt ggccagaacg gaggagctat
    16621 ggcggagaac atccggacac ccaacctgca gtacctggat gccgggtacg tcgcggacga
    16681 ccgcatcgac ttcgacggcc tcgacgtgcg gaacgccgcc ggtgacaagt tggggtcggt
    16741 cgacggcttc atcgtccaga ggaactcgaa tcgcccgtac tacctggtgg tcgactcagg
    16801 cggctggttc tcgagccgtc actacctggt gccgatcggg cacgtgcggc tggacccgga
    16861 caaccgcgcg cttcgcgtgg acatcgaacg ggacacgatc cagggattcc cggaggtcca
    16921 catcgatcgg ttcgacgagt tcaccgagga agaggtccgg cggatcgacg agcggacgct
    16981 caccgcgctg gcgccgaacg aggtcgccgc gcgcatgggc gacacgtggg actacgaccg
    17041 gtggagtcac tacagccaac ccgactggtg gcgggcccag ccggccgatc tgccgccccc
    17101 gccgctcacc aaccggccgc tggccgagga ctacgccccg gacgtcgcag gcaccgacgc
    17161 cgaggtcacc ggatacgcgc cggagcacat caccgcccgg cacgacgaag acctggcacg
    17221 caggacggat cgccgcgacc tcgaggggct cggcaccatc cgccgttccg atacggcagc
    17281 cggcacgcca ccggaggacc tgaacgaccg cgcgcagccg ggcgacgtgc tgggcatcga
    17341 gacggccggc gaaacgacct cgctcggcga cacggcccgc gacgaggaca agcgccgcga
    17401 ggaagcggag gaagccaacc gcgagatctt cgagaatcag cggcgcgacc ggcgccgcga
    17461 cggataggtg gttgcaggcg gcggccagca ggtgaaaccc ctgccggccg cccgcgtcgg
    17521 cggcctttac gagagcggct cgaaggtggc cgtgaagtgg cggagcacgg ccggcgagct
    17581 ggcgatccgg tagccgcgaa tcgagtcctt cttccgggcg acctcgatca cgccttcggc
    17641 cacgtaatcg atgtgggact gcgtgtagac gcgccgcggg atggcaagcc gcaccagctc
    17701 catcggggcg gcgacctcgc ttccgtccgg cttccgcccg aacatcagcg tcccgatctc
    17761 gactccgcgg atcccggcct cgacgtacag ggcgttggcg agcgccacgc cggggtactc
    17821 gagcggcgcg acgtgcggca gcagcgcctt ggcgtcgagg tagatggcgt ggccgcctgc
    17881 cggccggatc gtcgggacga gggcggcggt cagtttgtcg cccaggtact cggtggaacg
    17941 gatccggtac ctgaggtacg gctcctcgac cacctcttcc aggccgcgcg cgattgcctc
    18001 caggtcgtac ccggcgaggc cgccgtaggt cgggaacccc tcggtgagga tcagcaggtt
    18061 gcgggcctcc tcggcccagc gatcgtcgtt gagcgcgagg aagccgccga tgttcgcgag
    18121 cccgtccttc ttggccgaca tggtgcagcc gtcggccagc gagaagagct cctgcgcgat
    18181 cgccttgggc gttcggcccc cctgccctgc ctcgcgcacc ttgacgaagt acgcgttttc
    18241 ggcgaaacgg caggcgtcga ggaagaacgg cttgcggtag cggtcgcaga gggcgcggat
    18301 gccgcggagg ttctcgagcg acaccggctg gccgccgccc gagttgttgg tgatggtcgc
    18361 catcacgagc gggacgcggt cgcccgcctc ggcgagcgtg cgctcgagtg ccgccaggtc
    18421 gacgttcccc ttgaacgggt ggaccagggc ggggacacgg ccctcggaga tcaccaggtc
    18481 gcgcgcctcg cacgcctcgg cctcgatgtt ggcgcgggtg gtgtcgaagt ggttgttgct
    18541 cgggacgatc tggcccggcc gcagcaccgt gtggaaaagg atgtgctcgg cggcccgacc
    18601 ctggtgggtg gggatgacgt gcttgaagcc ggtcaggtcc ctcaccgcgg cctcgaagcg
    18661 gtagaaggac cggctcccgg cgtacgactc gtcgccctgc atcagcgcgc cccactgcgc
    18721 cgccgacatg gcgccggtcc ccgagtcggt cagcaggtcg atcagcacct cggcggcggg
    18781 cagccggaag acgttgtagc cggccgcccg cagcgcctcg gcccgttcct cgcgcgtggt
    18841 gaaggcgatc gcctcgaccg acttgatccg gaacggttcg atgatcgtcc tgaagttcat
    18901 ctcaggcttc ccacgggagc cctgtcttgc cgaagtggcc gtagttggtc gtcgcccggt
    18961 agatggggcg cagcaggtcg agcgactcga tgatcgcggc cggcctgaag tcgaaccggt
    19021 ccaccatctc gcgggccgcc ttctcgtcgc cggttccgaa ggtctccacc ttggtcgaga
    19081 ccggctgcgc cttcccgatc gcgtacgcca cctgcacctc ggcccgccgg gcgatgccgg
    19141 ccttgacgac ctggcgcgcg acgtaccgcg cgaagtacgc gccgctgcgg tccaccttcg
    19201 aggggtcctt cccgctgaac gcgccgccgc cgtggtgcgc cgcgccgccg taggtgtcga
    19261 cgatgatctt gcggccggtg acgccgcagt cggccgacgg accgccgagc gcgaagctgc
    19321 cggtcgggtt cacgagcacc ttcacctcgg gcgtgaacca cgagtcgagc acgcggggcg
    19381 ccaggcaggt cgccacgtag cgctcgatct ccgcgtgcgg cgtgtcctgg tcgtgctgcg
    19441 tcgacaccag cacggtggtc acgcgggccg gccggccgtt cgcatactcg accgacacct
    19501 gcgatttcga gtcgggccgg atccacgggt acttcccgct gcggcggtcc tcggcgagcc
    19561 cccgggtgag gcggtgcgcc aggacgatcg gcagcggcat cagctcgggc gtctcgtcgg
    19621 tcgcgtagcc gaacatcatg ccctggtcgc cggccccctg ctcgacgtcg aggccgcggc
    19681 cctcgtccac gccctgcgcg atgtcgtccg actgccggga gatgaactga agaaccttga
    19741 cgcggtcggc gtgaaacgcc tcgccgtcga acacgtaccc gatctcccgg atggcggcgc
    19801 gcgcgatgcc ctcgtagtcc accggcgtcc tcgacgtgat ctcgccggcc agcaccacct
    19861 gcccgtcctt gcacagcacc tcgcaggcca cgcggctccg cgcgtcgccc gagaggtgcg
    19921 cgtccaggat cgaatcggcg atgtagtcgc aaaccttgtc ggggtggccc tccgacacgg
    19981 actccgaggt gagcgtgtgg gtcagtttct ccatagtcct tcctcgtgct gggatgacgc
    20041 cctttcgaga tcgcggtccg tctcgctcgc gcatggctcc aggccacctg gcctggccgg
    20101 ctgggccacc gttcgctccg ggtgagggag caggacgacg gtatggcgta cgggtcgtcg
    20161 agccggaact ctcgccgtac tcttcatgct ggcagcccga gaccaggagc tgccacaaag
    20221 ggctggcacg cggcgccgac gcagcactcg ccgcgcagtt ttccactatg gcggctcagc
    20281 tcgggggctg tcaagcggga tgatgaagct cgtcgtgctg ggggtgcagg cggtgcagga
    20341 ggtgctggag gtgcagtgct ggaggtgctg aaggtgcggt gctggaggtg ctcaaggtgc
    20401 tgtcggtaat cacgacctag ggacccctgc ctgacaggtt tctcaccagc attcccccct
    20461 gacggaccgg tgtttctcac cacctttccc ccctgtgcgt gacgcagcga tcatccccct
    20521 tccgacccgg cccccggggc gggctggagg aggggatggc gtatcgcgag gtcacgatga
    20581 tcgaagtgaa ggaggtgttg cgcctgtggt ttgccggcac ggcgaccaag cgcatcgccg
    20641 cgatactggg gctcgatccg aagacggtcc gtcgctacct gaaggtcgcg ggggacgcag
    20701 gggtgctccc gggcgcgacg gcggtcaccg atgctcaggt gacggcggtg ctgctcgcac
    20761 tgcatccggt gattgagcga ccgcacggcg acagctgggc ggcatgcgtg aagcaccggg
    20821 cggacatcga gaaatggctc aagcaagagc tgcggctgac caaaatccgc acgctgctgg
    20881 gccggaaggg cgtgaaactg ccctatgcga ccctccaccg gtatgcggtg agcgagctgg
    20941 gcttcggccg ccgcgcggcg acggtgggcg tcgtggatgg cgagccggga caggagctgc
    21001 agctcgacac ggggtgggtt ggctggctgc gggggacaac gcgtcgtcgc ttccgcgcgt
    21061 ggatcttcac ggcggtccgc tctcgacacc gctttgtcta tcccatcttc cgggaaacga
    21121 cggccacggc gatcgaagcc tgtgaggccg cgtgggagtt ttacggcggc gtcttccgcg
    21181 tgctgatccc ggacaacacg aaggcgatcg ttcacaccgc cgacaatctc gagcccaaac
    21241 tggtcgatgc gtttctcgag tatgcgcaag cgcgcggctt ccacgtcgat cccacccgcg
    21301 tccgccatcc acaggacaag ggccgggtcg agcgggcggt ggcgtcggtg cgcgacgatt
    21361 gtttcgccgg tgaagagctg atcgacctcg acgccgcccg gacctgcgca cgccgctggt
    21421 gtctcgagga gtacggccac cgccggcact cgcgcaccca gcgccggccc ctcgagcatt
    21481 tcgaggccga ggagcacgcc gccctccagc cagccccaat cgaggcgtac gacctcccgg
    21541 cctggtgcga ccccaaggtc ggccgcgatc actacgccgt cgtggcgtgc gcgttgtact
    21601 ccctgcccac tcggtggatc ggcgtgcgtc tgcgcgcccg cgccgaccgc gcgaccgtcc
    21661 gcttctatca ccgcggcgtg ctgatcaaga cgcatccccg ctgcccgccc ggcggcaagt
    21721 cgacgcacgc cgaggatttt cccgcggagc ggaccgccta tgccctccgc gacgtcactt
    21781 acttgcagac gcaggccgac gggcacggcg aggcggtcgg ccgctacgtc cgcaccctct
    21841 tggatggccc gctaccctgg acgcggatgc gccgggtcta tgccgtcctc ggcctcgccc
    21901 gccgctacgg cagccgacgc gtcgacgagg cgtgccggct ggccctcgtc gcgggatgct
    21961 cgacgtccat cgcctccaac gcatgctcgc cctcgcggtc accgccgcgc cgtccgctcc
    22021 cgcgccgccc gctccggccc ccgcacgctt cctgcgtccg gccgcgcagt accgcctggc
    22081 gctcgtgccc gccacgcccc cgactgaagg agagcctgaa tgaaggatcc gatttcgccc
    22141 gatctccgcg ccgtcctccg ccgcctcaaa ctcggtcgga tgctcgacac gctgcccgag
    22201 cgcttcgccc tcgctcgaca gcagcagctc gcccaccagg acttcctgct cgtcgtcctg
    22261 ggggacgagg cgtcgcgacg cgatgccctg agcgtcacca cgcgcgtcca gcgagcccac
    22321 ctcgatccga ccatgcagct ggaggcctgg gaccccacgg ccaaggtcac cttcgacaag
    22381 acgctgctca atgacctcgc ctccctccgc ttcctggacg cccatgcgca tgtcgcgatc
    22441 gtcggccccg tcggcgtcgg caagaccttc ctcgcccacg ccctcggata cgccgcctgc
    22501 cggcggggcc gctcggtcct cgccttgcgg gccgaccgga tgctcaaaac gctcaagcac
    22561 gcccggctcg accacactta cgaggcggaa ttgcgaaagc tcctcgccgt cgatctgctc
    22621 atcctcgacg atttcgccct cgacacgatg gacccgaccg agagccgcga catctacgag
    22681 ctgctgaccg aacgccaccg ggtcggctcg atcatcgtca cctcgaaccg cgcgccggac
    22741 gaatggctcg cgacctttgc cgaccccgtc cgcgcccaaa gcgctatcga ccgcttcacc
    22801 agcaacgcct acgacctgct catcgagggc gagtcgtacc gcagccggca gaagccgggt
    22861 cgccgatcgg gggcggcggc atgagcgtgc gcacacccgg gctgtggaaa tggcgaccct
    22921 gtggaaaccc cagaacggtt tccacaaggg tcttggacat cgcgcccaga gcgcgcgatg
    22981 cc
//
LOCUS       DAOPQX010000015        22373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2889_length_22373_cov_4.963930, whole genome shotgun sequence.
ACCESSION   DAOPQX010000015 DAOPQX010000000
VERSION     DAOPQX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 22373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22373
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2889_length_22373_cov_4.963930"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKK95_01695"
     CDS             <1..227
                     /locus_tag="PKK95_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16945.1"
                     /translation="LYRGGAPVKPHDLCQAVAVSDADLVATYLNIIDARTVDCAGLSV
                     LERARRLSPSAKRDTILRQLTSVLTQRTPR"
     gene            588..1166
                     /locus_tag="PKK95_01700"
     CDS             588..1166
                     /locus_tag="PKK95_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOC16946.1"
                     /translation="MLKQTKDGDLQGIEASLKAGADANFVPDPVGAESPLINAARRGH
                     VAAVRLLLQHGALPNMRTTDETPLHAAAARGHADVVALLLAWGANYRALIGFGGQHAL
                     GDAVSGGHGEVVWLLYKEGATIQPVFLCYAVSDGHAELVNVLLNTGLDPRTVDCSGRS
                     PFDLAGRLPLPQREEISRRLARARSSTSDPNR"
     gene            2028..3992
                     /locus_tag="PKK95_01705"
     CDS             2028..3992
                     /locus_tag="PKK95_01705"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOC16947.1"
                     /translation="MRRRLLAGASLGLAAAVLAFLVGLVPFAETVELKTYDWRIRLTA
                     SPSEARRDIVLVAIDQASIRSLEPLVGRWPWPRFIHGTLLDFLARAPAKAVVYDVIFT
                     ERDRRRFTVGGEEWTGEESDQALVEATRRAGMVVHVADVAGEATEGGEATAAPALPAA
                     FRVSGFERRPSLVLPFPELAEASRALGHNLVILDADGPARRAAPFVEHAGRPLPSLAV
                     AAAAIAERLGPGDVSSDGRTLKLGAHELPLVRQELPSFYGERGEARRALVRFPGGVLE
                     GGKPTYREHSFYDLFYSEQQLLAGEKPLVDPASLKDAIVLVGTTAAGLYDLFSVPFPG
                     KMPGMQVHASALDNLLSGRFLAPAPGWAGLLVLLACGLAAALAVTVLGVWRGLAAGAI
                     MVVALLGGSVLLFGAGTWLQVTPPFVAAVLASFSGVAYQYLVEDREKRKVKQLFSRYV
                     SKDVCEQLMADPSRAHLGGSRREMTVLFSDIRGFTTFSEAGQPEQVVEQLNEYFTRMV
                     EVIFAHRGTLDKFVGDAVMALFGAPLDDPGHAEHAVQAARAMLRELETLNRGWAAEGR
                     PTLAIGVGINTGEMVAGNIGSESIMSYTVIGDAVNLASRLESLNKQYGTSIIVSEATR
                     ARLEGDYGVRALGEVVVKGKTRAVPIFEVS"
     gene            4004..5113
                     /locus_tag="PKK95_01710"
     CDS             4004..5113
                     /locus_tag="PKK95_01710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOC16948.1"
                     /translation="MRNLAVACAACAILAPAALAPAAPADPQIGKILRRAEKRAEQLQ
                     SLRVTEQEEIELGAAVSERIRVRYGVVQDASVHKYVALVGTLLVEGGPRPGLPWHFIV
                     LDTDGVNAFAAPGGYIHVTRGALGLIRNEAELAAVLAHEIAHVTDKHTVEAIQKSNAI
                     QIGASETLRDKEVFSRVVDKTAEIVMAGFGRSEELEADRDALRRADAIGYAPGGLAAF
                     LTRLTERNKESTGKQGLYSSHPEMKERLERLGKQIAAEHLQATILLDERYARHVSYET
                     KPQADVAAVVDGAAGLTGGEAAPAPPPKKKGIGLSNLLKPSGSERKSAEVTGSGASRG
                     VDTERGAAGGSNPALVEVQLTAAEVSAFRKEGKLK"
     gene            5110..6534
                     /locus_tag="PKK95_01715"
     CDS             5110..6534
                     /locus_tag="PKK95_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOC16949.1"
                     /translation="MTHVAALVAIGAIAATLAVGGLVANRLIRRKLRLSLFSFLAYLL
                     FPATSAFAPALVQPFQSVGQLLLVLGFVNLAVVLAVNPWRVDRVPERFPSIVQDALVI
                     GAFLVVATVILQEKFLTTSAVGAVVIGFALQDTLGNTFAGLALQMEKPFRVGDWIQVG
                     SHDGQVTEITWRATTLRTRDDDAITVPNNIVSKEAILNFSRPVPHTMLHVEVGVSYDA
                     PPNRVKAVVLEALKDATYVLATPAPAVLVSSFGESSVNYRVEFWIGDPSLDDEARDEV
                     LSEIYYALRRANLEIPYPIRVEYQRESVPVALVPAERRREEIARVDIFSPLSAEAKVR
                     LADASSERLFGSGQTIVWEGQAGESMFVVVSGRVRVTLGNRANEVATIGPGGYFGEMS
                     LLTGDPRTATVSAIDDCLLLEITADDFRTIVLADPLVVERIGLAVAERRMGLERSRNE
                     AAAAVAVHEAPRSLLARIQRFLRL"
     gene            complement(6538..7047)
                     /locus_tag="PKK95_01720"
     CDS             complement(6538..7047)
                     /locus_tag="PKK95_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16950.1"
                     /translation="MARRNWIPIVVGIVIFVAIVGIGLIAGAIYLFSRQVEFQTVETA
                     HGEQEFRKLQAGFEGQTPFIELTGDGEFDEARAVVHRELAKGTTGEVSTVHIRVWSPE
                     EGKLISLDLPFWMVRLMGSKPINFETGEGGFNRVSLRVTPEELERRGPGLIMNHTAPR
                     GESVLVWLE"
     gene            complement(7078..8283)
                     /locus_tag="PKK95_01725"
     CDS             complement(7078..8283)
                     /locus_tag="PKK95_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC16951.1"
                     /translation="MKNGARPHFRAALGAAALLIAADAYAGVQPQDLVRRAAEATYNL
                     DYEQALALYRQALAIDPESAATHRGIASLAWLQVLFLRGTVTVDDYMGKLASSEIKLE
                     PPPPALAQLFHHHIDRAIELGEQAVRARPSDAGAHYALGAALGLAASYQGSVEGKLLP
                     AFRAARRAFDAHEKVLKLDPSRKEAGLVVGTYRYAVSTLPAPLRWMAYLVGFGGGRDE
                     GIALLEAAAAHPSEAQPDARFALVLVYNRERRYDDALRVVRQLEQSYPGNRLLWLEEG
                     STALRARRNEEARRALQDGIAKLASDPRPRMPGEEAYWRMKRATALVLLKQPEPARDE
                     LVAALADADARMWVRARAHLEMGKLADLAGDRTAARREYQAAATLGERSNDPQTRDLA
                     KRLAKNGYK"
     gene            complement(8535..9065)
                     /locus_tag="PKK95_01730"
     CDS             complement(8535..9065)
                     /locus_tag="PKK95_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16952.1"
                     /translation="MTWLIVLATIVVVGLAAYFFSRVAAGRGPRLVECPENDEPAAVS
                     VNAVKAAVGGRLELSRCSRWPERGQCGRECLTQIERSDDGCLVRTMVTSWYDGKSCVM
                     CGRAIGDVDWLDRKPALAGPDGGLRQWQEVAPETLPTVLATHRPVCFDCYVSETFRQQ
                     HPELVIDNPWPDATRQ"
     gene            complement(9183..10076)
                     /locus_tag="PKK95_01735"
     CDS             complement(9183..10076)
                     /locus_tag="PKK95_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC16953.1"
                     /translation="MDSPRLLASALAVGLLSHAALGLAARQAPTFRGDTRFVPVYATV
                     VDRDRRLVPGLTRDDFEVFADGKPRPITVFSNEIEPITVVVMLDTSASMTPNLGLVTA
                     GAEQFFLRLLPGDQAVAGAFNDKIEFASDFTSDRDELIASLRSLDFGNPTRLYDAVAL
                     SLEQLQRVEGRKVVLVFTDGADTSSSASLGDVLARARAEEVMVYAIGLEADYFNGVRQ
                     VRSKPDRGLRKLAEETGGGYFELDKKDLLTSTFTRVSEELHSQYLIGFDAGARDGKVH
                     KLEVRVKKAGATARARKSYVG"
     gene            10133..10558
                     /locus_tag="PKK95_01740"
     CDS             10133..10558
                     /locus_tag="PKK95_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOC16954.1"
                     /translation="MANEIPVMVARLEWDGELQFTGFVGKHEVPIDGSQHAAASPMQL
                     LAMSIAGCMAIDLVHILARGRHDCTSLEVGFRAERAADHPRRFTRVTLHFAMSGAMTP
                     EHVERALRLSREKYCSVWNSVRPDTELETTFEILAAPAA"
     gene            10555..11688
                     /locus_tag="PKK95_01745"
     CDS             10555..11688
                     /locus_tag="PKK95_01745"
                     /inference="COORDINATES: protein motif:HMM:NF021633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOC16955.1"
                     /translation="MSEVARAGGAGQGTASRGAARLSYVDWVRGLAILVMIQAHSLDA
                     WTRVEDRATAGYGWSALIAGMGAPMFLFLAGVSVALAAPGRERKLGGPARAAASIRRR
                     GWEIFGLAFLFRLQSYVLNAGTTLTGLLKVDILNIMGPSIVGAAALWQAGRRYGPRLA
                     LFAAAALALTLLTPPVRGAGFLSVIPDPIEWYIRPAPGRSVFTLFPWAGFVFAGGVVG
                     VLIAGRASAAGAGRSSDRWPFQVALFAGSLLLVAGAYGASFLPSIYSNSSFWTTSPTF
                     FLMRVGIVSALLPLAWFYERRPRLLARWISWSPMALFGQSSLFVYWVHTELAYGSASL
                     PLHRRLPFGQALVAFGLLTLFMFVLTLGKNALVRAWRERRATV"
     gene            complement(11913..13307)
                     /gene="gatD"
                     /locus_tag="PKK95_01750"
     CDS             complement(11913..13307)
                     /gene="gatD"
                     /locus_tag="PKK95_01750"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOC16956.1"
                     /translation="MSTAADALKGYKGRARAALEAWGVRVWSDVQVENDAGSRFEGVI
                     LPRSETLDDLHVVLKLKNGYNVGLHVDRIARVSEVGYKEAVYKIPEKEFPRRPDLPSV
                     TLLGTGGTIASRLDYRTGAVIPAFTPGELYGAVPELADICNLTTKKLFGVFSENMAKE
                     QYVALARAIGEEIEGGADGIVIGHGTDTMGHTAAILSFMVQDSPVPIVLVGSQRSSDR
                     PSSDAALNLIHSVRSAAYGEIAEVQICMFGPTSDRYGLLHRGTRCRKMHSSYRSTFRT
                     VGDIPLAMVSRDSFTYLHRDFRRRDNSRRVKIDAVYDDRTTILYYYPGMSPDLVDALV
                     EKGYRGIVIAGTGLGHVNKPLYPALKRAIEAGVHVVMTVQTLWGYAQMYVYDTGRDLL
                     ELGIVPLENMLPETALMKLSWVLAHTDDHGEVMRMMRTPVSHEITEREPHNGYLIMQG
                     GLPEIEEFILSHWK"
     gene            complement(13304..15262)
                     /gene="gatE"
                     /locus_tag="PKK95_01755"
     CDS             complement(13304..15262)
                     /gene="gatE"
                     /locus_tag="PKK95_01755"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOC16957.1"
                     /translation="MESIDYADVGLIAGLEVHQQLLTERKMFCHCPARRYTRDHDGEV
                     LRHMRPTLSELGEYDGTALMEFKTRKNIIYLLHEENVCTYEMDDTPPFLVNQEAVDVA
                     IEQCLMLGCDIVDEVHIARKQYLDGSIPTGFQRTAIVGVNGRLPFRGRELSILQVSVE
                     EDSCREVSDKGHLIVWRTDRLGMPLIETVTGPDLRTPDEVAEAILLIGRVCRSTGHVR
                     VGIGASRQDVNVSVRGGRRVEIKGVPKAGWAPGLVHGEAVRQVNLLRLREELIRRGFS
                     APADIGCESADVTRLFASSEQSFLRREGWERFVAEEKRRAGFELGNGPFTVRAVRLKG
                     LTGTLGWPTQPDLTFAHELAGRVRVIAGLDQRPILLHGEKWPDYHGSLQELRRVRAHL
                     HCNPEDAIAVVWGPGEDTLTAAAEIRLRYVDALDGVPNETRQPFPDGSTDFERILPGP
                     DRMYPDTDSPPSRVTRERVERLRAALAPRPWEREARYTAAGVPRQTAYFLIRRGAARL
                     VDMVVDGCGAPLRRAAELFGERLKGLRREGVAVDAIPDERWCEFFREAARSAVIWQAW
                     DVVVRAMAERPGVPVAALLAEKRLGCAPDGWEEIPVAALAAARARAHRHDTGLLARLA
                     MGIAMGDLRGRVPAERVAGAIRTEMAGR"
     assembly_gap    15387..15486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15580..18282
                     /gene="ppc"
                     /locus_tag="PKK95_01760"
     CDS             15580..18282
                     /gene="ppc"
                     /locus_tag="PKK95_01760"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOC16958.1"
                     /translation="MASSDPEGPLRADVSLLGTLLGDVVRDREGVEVFETVEQVRRLA
                     KAARAGDEQAFETLRARVLGTLPLDEALPVARAFAHFLGLANIAEQHHRIRRRRQYLA
                     DPATAAQPGSFEEALPRLLAAGVTPADAQAALERLSIGLVLTAHPTEVSRRTILLKQN
                     RLAALLAERDRPDLTAWERDENLDSLRRVIQELWETDEVRRGRLSPLDEVRWGLAIFE
                     QTLWDAMPRFLRSLDRAAGRHLGAALPVTAAPIRFGSWMGGDRDGNPNVTPEVTWQTC
                     LMARWVAAGLYLREVDALRSELSMTAASDELRAQARGEREPYRVVLRQLAARLRATQA
                     WAAALLEYATPGPPAGPALAPGAAAMGVPADDPLLEDEELTGVFRLCYRSLVETGNGP
                     IARGRLLDACRRAEVFGLTLARLDVRQDAARHDEAMEAIAGGQGAWRTWDERRRLDFL
                     RPHLAGPSRLRLDVEAPREVREVIETFRVMARIPRSSLGAYVVTMASRPSDVLAVLAL
                     QQAAGVEPPLRVVPLLETGRDLQAAGEVIGGLLAVPEYRAAIGGRQEVMVGYSDSSKD
                     VGRFSAGWRLYRAQEEIVEACRLAGVRLTIFHGRGGSVGRGGGPTHLAIRALPPGAVD
                     ASLRVTEQGEMIQAHWGLPELAVRTMEVYTTATLEATLRPGRGPTREWRERTDALAKV
                     ARNAYQAIVHQHPAFVEYFRAATPEVEIGRLNIGSRPARRSAGKGVGSLRAIPWQFAW
                     TQTRLMLASWLGLEEALGGAAGPGDGKGDGLREMYREWPYFRVLLDLMEMVLAKADPA
                     IAAEYDRHLVPPHLQPLGEELRRRLALAIDRVLAATGHATLLEENHVLRRSIAVRNPY
                     VDPINLLQVELLSRLRSESSPDERLWTAFAVTVNGIAAGMRNTG"
     gene            18307..18480
                     /locus_tag="PKK95_01765"
     CDS             18307..18480
                     /locus_tag="PKK95_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16959.1"
                     /translation="MGIRDQIVQEVPEVLEVQRVQVQEVLEVQRVQVPEVLEVPRVQV
                     QEVQRRWCSKCRC"
     gene            complement(18674..21412)
                     /locus_tag="PKK95_01770"
     CDS             complement(18674..21412)
                     /locus_tag="PKK95_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOC16960.1"
                     /translation="MSVRSRRFGLALLVVLALSSAGLVLPAAQAPQAADGEFARLVRE
                     WTTRPEFISPLVDYLPVAAGIPSPRDVIGHHVGEPKKLTYYEDILKYYRALAAASPRV
                     RVMEIGTSDEGRPLVVVAIASADTMAGLESYRERLARLADPRQLSEAQARQVIEEAKP
                     IYHVMAGLHSGETGPPEMLMELAYRLAADESPLYREIRDNVIVTFTPVADPDGRDRYV
                     DWYYAYKVDETGEDDSYGGPPYWGKYVRHDNNRDINYSQAITRAVLKWYLEWHPPVLH
                     DIHESVPFLFTFSGQAPQNPTLDPILYAELPWYANFEMAQAIKYGMPGVWTHAFVDMW
                     SPGYFGFMASNHNGMLRMYEVFGNGGANTMLRKIDRGDRGGGQTSREWYRPLPPYKEV
                     EWSLRNNVNYGQTAVLSALQLTSRFPKIVLENFYRKSWNAVEAGTKNAPYAFVIPAGQ
                     ADMTRVAFVADVLIAQGIELGRTRGEVKIGDLAFPAGSLVVRLDQPYGRLAKILLEKQ
                     DFPDPNLRTYDDSGWTMGLLTHTEVKPVADKAILAVPTDPVDRFTAKGRVDGKGEGAG
                     WIAAAANGSANLVSLRFELRSMDVHAASKAFAAGDTRLPAGSLLIEVRGAAERRKAVE
                     AIERLGLVAVSLGGRPDVPTHAVDLPRVAVFSTWGGTQEVGWVRYSLDRFEVPYELIF
                     KERVRQGDLRSAFDLIIVPSQSGSAKRLVFDLEPKSRPLAYRKTDRFRNLGMYGESDD
                     ITGGMGLEGVMELRTFVEKGGLLVTMGAASYFPPEFGLARRVDASRPGPQFYAPGPVV
                     QVELLRPDHPIFYGYDKKVLPVRWAGGPLLRVPDTDKGQVLMQFPGGDASVLSGLMKG
                     AGEIKERPAAIDVPLGDGRVLLFATNPCYRWQNLGEFNMLFNAILHWNDFVSRGRE"
     gene            21504..22034
                     /locus_tag="PKK95_01775"
     CDS             21504..22034
                     /locus_tag="PKK95_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16961.1"
                     /translation="MRLATLAAVLVSGFLSSGCLVIGLHPFYDDGSIEFDEALIGEWE
                     SGEESLRIARSPWKSYRIDYTSGAETASYDGHLTRLGSFKLLDLVPVTEGAIRVHLPV
                     RVKPLGDTLSLAPLDFWWFRREAEHGQPQALRPAIDDEGDVLLTAATAAVRDWLAAHA
                     EHFDDDPVRFTRKPAR"
BASE COUNT         3237 a         7774 c         7755 g         3507 t
ORIGIN      
        1 tcctgtaccg cggcggagct ccggtgaagc ctcacgacct ttgccaggcg gtcgcggtca
       61 gtgatgccga cctggtcgcg acgtatctga acatcatcga tgcgcgcacg gtcgactgtg
      121 ctgggctttc cgtccttgaa cgggcccgcc gcctgtcgcc ttccgccaag cgggacacga
      181 tcttgcggca gttgacttcg gtgctgacgc aaagaacacc gcggtgatgg cgactcgcgg
      241 ttcgaggtcg ggaggcctgg aacgatgggg cgcgccggtt ctggtgctgg gcggctgata
      301 gtgacgcgcc tgtagaacgc tgcgagcgaa cgggcaacga cccggcggta gcacgtctga
      361 tacggaggcg ccggtagaac cgcgcgaggg agcgcgcagt atgtcggcgg agcgaatagc
      421 gctccatctg cagaatgtga accgagagga ccgtggcaac ctacccagtt cagcatcggc
      481 tggaagcact ggcgctcgtc attggcatct gtatggccgc ttgtggcggc gggccgcacg
      541 accgtccccc tctcccttcg ggaccgccaa tcgaacccaa cgtcgcgttg ctcaagcaga
      601 ccaaggacgg cgatcttcag gggatcgaag cgagcctgaa ggccggggcg gacgcgaact
      661 tcgtgcccga tcccgtgggg gccgagagcc cacttattaa cgccgccagg aggggccacg
      721 tggcggctgt tcgacttctc ctccagcacg gcgcgcttcc caacatgcgg acgactgacg
      781 agactcctct acacgcggca gcggcccggg gacacgcgga cgtggtcgcc ttgcttcttg
      841 catggggagc aaactaccgc gcacttatcg gatttggggg acagcacgca ttgggtgacg
      901 ctgtgagcgg cgggcatgga gaggtcgtgt ggctcctcta caaagagggg gcgaccatac
      961 agccggtgtt tctctgctac gccgtttcag acggtcacgc cgagctagtc aatgtcctgt
     1021 tgaacaccgg cctggatccg agaacggtag actgctccgg gcgctcaccg tttgacctcg
     1081 cgggccgtct gcctttgccg cagcgcgagg agatctcgcg gcgcctggcg cgcgcgcgct
     1141 cgagcacttc agatccgaac aggtagccgg acgacggcgg cacgcgccgc ctttgcggta
     1201 accaaacggg gggaacgaag tcagccagcc ggagctcttc tggttcgctt cgacggctct
     1261 cacgacttga aagaaacttg aaactttgcg cacgaagggg cctgtcggcg ccctacgcca
     1321 ccgaagggtt cgacgggatt tcctaagaaa accgacgcag ccggatgccg tcggagcatc
     1381 cggccgcgtt tctcagccac gggaccgggg ttcgaatccc cgtaccggta ccaaccatca
     1441 agctcacttc cctcagttcc ttctgaagcc ccggcacatg cggcaggcag ctcctgcact
     1501 ggcgcgctgc gatagcatga gcatgacggc atcttccgcg tgctgttgtc accggaccac
     1561 ctgcaggacg attgcgcttg ccctcggacc tacaatgcgc caggtgctga cgaccgaacg
     1621 ctgactgccg ccgacacggc gatgctccaa ggacgtccga tacagacccg gacgcacgcc
     1681 cggtcgttgt tccgcatcgc ggcattcgcg gtcctgatgc cgctcgcggc cactggggga
     1741 gccacggcac aggcagatgc tgatgcacgg cgcgtgtcct tggttgttct ggcggatccc
     1801 gatgacgcgt tcctcccact ggctcgcgag atcgccgcgg ccgaacacgc tcccttgacg
     1861 gccaccgtgc tcggcagcct tcgtggttgt ctttgcgcgc gaacgcgccc accggcccgg
     1921 acgcgtgcca acctcatcct gaacgccgca gccgcggacc tgagacctcg tgacttcgtg
     1981 actccgcgac tccgtggact gcccagcccg tgctagtctg tcgttccgtg cggagacgac
     2041 tcctcgccgg cgcctctctc ggccttgcgg ccgctgtgct ggccttcctg gtcggtttgg
     2101 tcccgttcgc agaaaccgtc gagctgaaga cgtacgactg gcggatccgg ctcaccgcct
     2161 ctccgtccga ggcaaggcgc gacatcgtcc tggtggccat cgatcaggcg agcatccgca
     2221 gcctcgagcc gctggtcggg cgatggccgt ggcctcgctt catccacggc acgcttctcg
     2281 acttcctggc ccgggcgccg gcgaaggcgg tggtctacga cgtgatcttc accgagcgcg
     2341 accggcgccg gttcacggtt ggcggggagg aatggaccgg cgaggagtca gaccaggcgc
     2401 tggtcgaggc gacgcgccgg gcgggcatgg tcgtgcacgt ggccgacgtg gcgggcgagg
     2461 cgaccgaggg cggcgaggcc acggcggcgc ccgccctgcc ggcggcgttc cgggtgtcgg
     2521 ggttcgagcg ccgcccctcg ctggttctcc cgttccccga gctggcggag gcgagccggg
     2581 ccctcggcca caacctggtc atcctcgacg cggacggccc ggcccggcgc gcggcgccct
     2641 tcgtggagca cgcgggccgg ccactcccgt cgctcgcggt cgccgccgcc gccatcgccg
     2701 agcggctggg gccgggcgat gtctcgtcgg acggccgcac gctgaagctg ggcgcgcacg
     2761 agctgccgct ggttcggcag gagctcccga gcttctacgg cgagcggggc gaggcgcgcc
     2821 gcgccctggt gcgcttcccc ggcggcgtgc tcgagggcgg gaaaccgacc taccgcgaac
     2881 actccttcta cgacctgttc tactcggagc agcagctgct tgcgggagag aagccgctcg
     2941 tcgatcccgc ctcgctcaag gacgccatcg tcctggtcgg gaccacggcc gccgggctgt
     3001 acgacctgtt ctcggtgccg tttcccggga agatgccggg catgcaggtg cacgccagcg
     3061 cgctcgacaa cctgttgtca ggccgcttcc tggcgcccgc gcccggctgg gcgggcctgc
     3121 tggtgctgct ggcctgcggc ctcgccgcgg cgctggcggt caccgtgctc ggcgtgtggc
     3181 gggggctggc ggcgggggcc atcatggtcg tggcgctgct cggcggctcg gtgctgctct
     3241 ttggtgccgg cacctggctg caggtgacgc cgccgttcgt cgcggcggtg ctcgcctcgt
     3301 tcagcggggt cgcctaccag tacctggtcg aggaccgcga gaagcgcaaa gtcaagcagc
     3361 tcttctcccg atacgtctcg aaggacgtct gcgagcagct gatggccgac ccgtcgaggg
     3421 cgcacctggg cgggtcgcgg cgcgagatga ccgtgctctt ctccgacatc cgcgggttca
     3481 cgactttctc cgaggccggg cagcccgaac aggtcgtcga gcagctgaac gagtacttca
     3541 cgcggatggt cgaggtgatc ttcgcgcacc gtggcacgct ggacaagttc gtcggcgatg
     3601 cggtgatggc gctcttcggc gcgcccctcg acgaccccgg ccacgccgag cacgcggtgc
     3661 aggcggcccg cgcgatgctc cgcgagctgg agacgctcaa ccgcgggtgg gcggcagagg
     3721 ggaggcccac cctcgccatc ggggtcggca tcaacacggg agagatggta gccgggaaca
     3781 tcgggtcgga gtcgatcatg agctacacgg tgatcggcga cgcggtgaac ctggcgtcgc
     3841 gcctcgagtc gctcaacaag caatacggga cctcgatcat cgtctccgag gccacgcgtg
     3901 cgcggctcga gggggactac ggcgtgcggg cgctcggcga ggtcgtggtg aagggaaaga
     3961 cccgggccgt gccgatcttc gaggtgtcct gagaggaatt tagatgcgca accttgcagt
     4021 ggcctgcgcc gcctgcgcga tcctcgcccc cgcggcgctc gcgccggccg cgccggccga
     4081 tccgcagata gggaagatcc tgaggcgcgc cgagaagcga gccgagcagc tccagtcgct
     4141 ccgggtgacc gagcaggagg agatcgagct gggcgcggcg gtgagcgagc gaatccgcgt
     4201 gcgctacggc gtcgtccagg acgcgtcggt ccacaagtac gtcgcgctgg tcgggacgct
     4261 gctggtcgag ggcggcccgc gccctggcct gccgtggcac ttcatcgtgc tcgacaccga
     4321 cggcgtcaac gcgttcgcgg ccccgggcgg gtacatccac gtgacccgcg gcgcgctcgg
     4381 gctgatccgc aacgaggccg agctggccgc ggtgctggcg cacgagatcg cgcacgtgac
     4441 cgacaagcac acggtcgagg cgatccagaa gagcaacgcg attcagatcg gcgccagcga
     4501 gacgctccgc gacaaagagg tgttcagccg cgtggtggac aagaccgccg agatcgtgat
     4561 ggccggcttc ggccggtccg aggagctcga ggccgaccgc gacgcgctgc ggcgggccga
     4621 cgcgatcggc tacgctcccg gggggctggc cgccttcctg acccggctca ccgagcggaa
     4681 caaggaatcg accggcaagc aggggctcta ctcttcgcac ccggagatga aggaacggct
     4741 cgagcgcctc ggcaagcaga tcgccgccga gcaccttcag gcgacgatcc tcctggacga
     4801 gcggtacgcc cgtcacgtca gctacgagac gaagccgcaa gccgacgtgg cggccgtcgt
     4861 tgacggcgcc gcgggcctga ccggcggtga ggcggcgccg gctccgcctc cgaagaagaa
     4921 gggcatcggc ctctccaacc tgctgaaacc ctccggctcc gagcggaagt cggccgaggt
     4981 gaccgggtcg ggcgcctcgc gcggggtgga caccgagcgc ggggcggccg gcgggtcgaa
     5041 ccccgcactg gtggaggtgc agctgacagc cgccgaggtg tcggcgttcc gcaaggaagg
     5101 gaagctgaag tgacgcacgt ggccgcgctg gtggcgatcg gcgcgatcgc cgcgacgctc
     5161 gcagtcggcg gcctggtggc gaacaggctg attcgccgca agctgcggct ctcactcttc
     5221 agcttcctgg cctacctgct gttcccggcg acctccgcct tcgcgccggc gctcgtccag
     5281 ccgttccagt cggtcgggca gctcctgctc gtgctgggct tcgtgaacct ggcggtggtc
     5341 ctcgcggtga acccctggcg ggtcgaccgc gtgccggagc gcttcccgag catcgtccag
     5401 gacgcgctgg tcatcggcgc cttcctcgtc gtcgccaccg tcatcctgca ggagaagttc
     5461 ctgaccacgt cggcggtcgg cgccgtggtg atcggcttcg cgctgcagga cacgctcggc
     5521 aacacgttcg ccggcctcgc cctgcagatg gagaagccgt tccgggtcgg cgactggatc
     5581 caggttggtt cgcacgacgg gcaggtgacc gagatcacgt ggcgggccac gaccctgcgg
     5641 acgagggacg acgacgcgat caccgtgccg aacaacatcg tgtcgaagga ggcgatcctc
     5701 aacttctccc ggccggtgcc gcacacgatg ctccacgtgg aggtgggcgt cagctacgac
     5761 gcgccgccga accgggtgaa ggccgtcgtg ctcgaggcgc tcaaggacgc cacctacgtg
     5821 ctcgccacgc cggcgcctgc cgtcctcgtg tcgagttttg gcgagtcgag cgtgaactac
     5881 agggtggagt tctggatcgg cgatccgagc ctcgacgacg aggcccgcga cgaggtcctg
     5941 tcggagatct actacgcgct ccggcgcgcg aacctcgaga tcccttaccc gatccgggtc
     6001 gagtaccagc gggagtcagt tcccgtcgcg ctcgttcccg ccgagcggcg gcgcgaggag
     6061 atcgcgcgcg tcgacatctt ctcgcccttg tcggcggagg ccaaggtccg gctcgccgac
     6121 gcctcgtccg agcgcctctt cgggagcggc cagaccatcg tctgggaggg gcaggcgggc
     6181 gagtcgatgt tcgtcgtcgt gagcggccgg gtgcgggtga cgctcggcaa ccgggccaac
     6241 gaggtcgcga cgatcggccc cggcggctac ttcggcgaga tgtcgctgct cacgggcgat
     6301 ccgcgcacgg cgaccgtcag cgcgatcgac gactgcctgc tgctcgagat caccgccgac
     6361 gacttccgca cgatcgttct agccgatccc ctggtggtgg agcgcatcgg cctcgcggtg
     6421 gcggaacgga ggatggggct cgagcgcagc cggaacgagg cggccgcagc cgtcgccgtg
     6481 cacgaggccc cgcgcagcct gctggcgagg atccagcggt tcctccgact ctagccgcta
     6541 ctcgagccag accagcaccg actcgccgcg cggcgccgtg tggttcatga tcagtcccgg
     6601 gccgcgccgt tcgagctcct cgggcgtgac cctgagcgag acgcggttga agcccccttc
     6661 ccccgtctcg aagttgatcg gcttgcttcc catgagccgg accatccaga agggcaggtc
     6721 gaggctgatc agctttccct cctccggcga ccagacccgg atgtgcacgg tcgagacctc
     6781 gcccgtcgtc cccttggcca gctcgcggtg cacgaccgcc cgcgcctcgt cgaactcgcc
     6841 gtccccggtc agctcgatga acggcgtctg accctcgaag ccggcctgga gcttccggaa
     6901 ctcctgctcc ccgtgtgccg tctcgacggt ctggaactcg acctgccggc tgaagagata
     6961 gatcgctccc gcgatcaggc caatccccac gatcgcgacg aagatcacga tgccgacgac
     7021 gatcgggatc cagtttcggc gagccattcg tctcctcgct cgtttcccgc ccccgcgcta
     7081 cttgtacccg ttcttcgcaa gccgcttcgc caggtcgcgg gtttgcgggt cgttcgaccg
     7141 ctcgcccagc gtcgcggcgg cctggtactc gcgccgggcc gccgttcggt cgcccgccag
     7201 gtcggccagc ttgcccatct ccaggtgcgc gcgggcgcgc acccacatgc gcgcgtccgc
     7261 gtcggccagc gccgccacca gctcgtcgcg ggccggctcg ggctgcttca gcagcacgag
     7321 ggcggtcgcc cgcttcatcc gccagtaggc ctcctcgccg ggcatcctcg ggcgcggatc
     7381 ggaggccagc ttcgcgatcc cgtcctggag cgcgcgcctc gcctcttcgt tgcgccgcgc
     7441 ccggagggcg gtcgatccct cctccaacca cagcagacga ttcccggggt agctttgttc
     7501 gagctggcgc acgacgcgca gggcgtcatc gtagcgccgc tcgcggttgt acacgaggac
     7561 gagcgcgaac cgggcgtccg gctgcgcctc gctcgggtgt gcggccgccg cctcgagcag
     7621 cgcgatcccc tcgtccctgc ctccgccgaa ccccacaagg taggccatcc accgcagcgg
     7681 cgcgggaagg gtggagacag cgtagcggta ggtcccgacg acgaggcccg cctccttgcg
     7741 cgaggggtcg agcttcagca ccttctcgtg cgcatcgaac gcgcgacggg ccgcgcggaa
     7801 cgcggggagc agcttccctt caaccgagcc ctggtacgac gcggccagcc cgagcgccgc
     7861 ccccagcgcg tagtgcgcgc cagcgtcgct gggcctggcc cgcacggcct gctcgcccag
     7921 ctcgatcgcc cggtcgatgt ggtggtggaa cagttgcgcg agggccgggg gtggcggctc
     7981 cagcttgatc tccgaggagg ccagcttccc catgtagtcg tcaacggtca cggtgccccg
     8041 caggaagagg acctgcagcc aggcgagcga ggcaatgccg cggtgggtgg cggccgactc
     8101 gggatcgatg gcgagggcct gccggtacag ggccagcgcc tgctcatagt cgaggttgta
     8161 ggtggcttcg gcagctcgtc gaacgaggtc ctgcggctgg acgcccgcat acgcgtccgc
     8221 cgcaatcagc agcgcggccg ccccaagcgc cgcccggaaa tggggcctgg ccccattctt
     8281 catgccagtc tcgaggggag ggcgacccgg cgacccgagg ggcgccaaac gccgggtcac
     8341 tccaaccggt cgcgggggcg atcgaccctc acgcgaacac cgccgcgacg atggcggcca
     8401 ccagcagcaa cgccaccgcc caatcagcct tgtcgtagat ccaaccgaac atctcacgcc
     8461 tccttgttgc ccactttctt agacgggcgg cggcgtgcga tggttcgtcg gccgcgcgcg
     8521 gcagcctccc aaccctactg tctcgtggcg tccggccacg ggttgtcgat gacgagctcg
     8581 gggtgctgct gccggaacgt ctccgagacg taacagtcga agcagaccgg gcgatgggtg
     8641 gcgagcacgg tcggcagcgt ttccggcgcc acctcctgcc actggcggag gccaccgtcg
     8701 ggaccggcaa gggccggctt tcgatcgagc cagtccacgt ctccgatggc ccggccgcac
     8761 atcacgcaac tcttgccgtc gtaccagctc gtcaccatcg tccgcaccag gcacccgtcg
     8821 tccgaccgct cgatctgggt gaggcactcg cgcccgcact gtccgcgctc gggccaccgg
     8881 gagcaccggg acagctcgag gcggccgccg acggcagcct tgacggcatt cacgctgacg
     8941 gcggccggct cgtcgttctc ggggcactcg accaggcgag ggcctcggcc ggcggcgacg
     9001 cgactgaaga agtacgcggc gaggccaacg acgacgatcg tcgcgagcac gatcagccag
     9061 gtcatgggta tcctccggag cgcagcctgc ccgaagctgc tacgacagtg tatacacccg
     9121 accaggggcc agcgactaga ggctgggggt ctaattcagg ccgcgtcttt cagcggcctg
     9181 ccctagccca cgtagctctt cctggcgcgc gccgtcgcgc ctgccttctt caccctgacc
     9241 tcgagcttgt gcaccttgcc gtcgcgcgca cccgcgtcga aaccgatgag gtactggctg
     9301 tgcagttcct cggagacgcg cgtgaaggtg gaggtcagga ggtccttctt gtccagctcg
     9361 aagtagccgc ccccggtctc ctcggcgagc ttccgcaggc cgcggtccgg cttggagcga
     9421 acctgccgca ccccgttgaa gtagtcggcc tcgaggccga tcgcgtagac catcacctcc
     9481 tcggcgcgcg cccgggcgag cacgtcgccg aggctggccg agctcgacgt gtcggctccg
     9541 tcggtgaaca ccaacaccac cttccgcccc tcgaccctct ggagctgctc caggctcagg
     9601 gccaccgcgt cgtacagccg ggtcgggttg ccgaagtcga gcgagcggag cgaggcgatc
     9661 agctcgtcgc ggtcgctggt gaagtcggac gcgaactcga tcttgtcgtt gaaggccccg
     9721 gcgacggctt gatcgccagg gaggaggcgc aggaagaact gctcggcgcc ggccgtcacg
     9781 agaccgaggt tgggcgtcat gctcgcgctc gtgtcgagca tgacgacgac ggtgatcggc
     9841 tcgatctcgt tgctgaagac ggtgatcggc ctgggcttgc cgtcggcgaa cacctcgaag
     9901 tcgtcgcggg tgaggcctgg aaccagccgc cggtcgcggt ccacaacggt cgcgtagacg
     9961 ggaacgaacc tcgtgtcgcc ccggaaggtg ggcgcctgtc gagcggcaag gccgagggcg
    10021 gcgtgcgaga gcaggccgac ggccagtgcg gaagccagga ggcgcgggga gtccatatac
    10081 gagcctagca tgggaaaggg gtcgctatta gaatccaagg cgaggagcag gcatggcgaa
    10141 cgaaatcccg gtgatggtgg cgaggctgga gtgggacggc gagttgcagt tcaccgggtt
    10201 cgtcggcaag cacgaggtgc cgatcgacgg ctcgcagcac gcggccgcct cgccgatgca
    10261 gctgctcgcg atgtcgattg ccgggtgcat ggccatcgac ctcgtgcaca tcctggcgcg
    10321 cggccggcac gactgcacgt cgctcgaggt gggcttccgg gccgagcggg ccgccgatca
    10381 cccgaggcgc ttcacgcgcg tcaccctgca cttcgccatg agcggcgcga tgacgcccga
    10441 gcacgtggag cgggcgctcc ggctgtcgcg cgaaaagtac tgctcggtct ggaactcggt
    10501 gcgccccgac accgagctcg agaccacgtt cgagatcctc gcggccccgg cggcatgagc
    10561 gaggtggcgc gcgccggcgg cgcggggcag ggcacggcct cccggggggc ggcgcggctg
    10621 tcgtacgtgg actgggtgcg cggcctcgcg atcctggtga tgatccaggc tcactcgctc
    10681 gacgcctgga cgcgcgtcga agaccgcgcg acagccggct acgggtggtc ggcgctcatc
    10741 gccgggatgg gcgcaccgat gtttctcttc ctcgcgggcg tctcggtggc gctggcggcg
    10801 ccgggacgcg agcggaagct tggcggcccg gcccgcgccg ccgcgagcat caggcgccgg
    10861 ggctgggaga tcttcggcct ggcgttcctc ttccgcctgc agtcctacgt gctgaacgcc
    10921 ggcaccacgc tcaccggctt gctcaaggtc gacatcctga acatcatggg gccgtcgatc
    10981 gtcggcgccg ctgcgctgtg gcaggccggg cgccgctacg gcccgaggct ggcgctgttc
    11041 gcggcggcgg cgctggccct cacgctgctc acgccgcccg tccgtggagc gggattcctc
    11101 tcggtcatcc ccgatccgat cgagtggtac atccgtcccg cgccggggcg cagcgtcttc
    11161 acgctgtttc cctgggccgg gttcgtgttc gcgggcgggg tggtgggggt gctcatcgcc
    11221 ggcagggcgt cggcggctgg tgccgggcgc tcctcggatc gctggccgtt ccaggtggcc
    11281 ctgttcgccg gttcgctgct gctcgtcgcc ggggcctacg gggcgtcgtt ccttccctcg
    11341 atctactcga actccagctt ctggaccacc tcgcccacct tcttcctgat gcgcgtcggg
    11401 atcgtgtcgg cgctgctgcc gctcgcctgg ttctacgagc ggcggccccg cctgctcgcg
    11461 cggtggattt cgtggagccc gatggcgctc ttcggccagt cgtcgctctt cgtctactgg
    11521 gtgcacaccg agctggcgta cggctcggcc agcctccccc tccacaggcg tctgccgttc
    11581 gggcaggcgc tcgtggcctt cgggctgctg acgctcttca tgttcgtgct gacgctggga
    11641 aagaacgcgc tggtgcgcgc gtggcgcgag cggcgggcaa cggtgtgaac tcgttcaccg
    11701 cagaagcaca gaggactgtc acggacgttc accgcagaaa cgcagagagc gcagaggatc
    11761 cctgataaga cgcagagatc tattgaacga tccggatcct ctgcatacgt ctctgcgtcc
    11821 tccgtgtctc tgcggtgaat gtcgtggacg agccgtgacg tctgttacgc acgtttcacc
    11881 gcgaagacgc agagggcgcg gacaattgct cgctacttcc agtgcgacag gatgaactcc
    11941 tcgatctcgg ggagcccgcc ctgcatgatc aggtaaccgt tgtgcggctc gcgctcggtg
    12001 atctcgtgcg acaccggcgt ccgcatcatg cgcatcacct cgccgtggtc gtcggtgtgg
    12061 gccagcaccc acgacagttt catcagcgcc gtctccggca gcatgttctc gagcgggacg
    12121 atccccagct ccagcaggtc gcggcccgtg tcgtagacgt acatctgggc gtagccccag
    12181 agcgtctgca cggtcatcac cacgtggacg cccgcctcga tcgcccgctt cagggcgggg
    12241 tagagcggct tgttcacgtg gccgaggccg gtgccggcaa tcacgatgcc gcggtagccc
    12301 ttctcgacga gcgcgtccac caggtcgggc gacatgccgg ggtagtagta caggatcgtc
    12361 gtccggtcgt cgtacaccgc gtcgatcttc acccggcggc tgttgtcgcg gcgcctgaag
    12421 tcgcggtgga ggtaggtgaa gctgtcgcgg ctgaccatcg cgagcgggat gtcgccgacc
    12481 gtgcggaacg tgctgcggta ggagctgtgc atcttccggc agcgggtgcc gcgatgcagc
    12541 aggccgtacc gatcggaggt cggcccgaac atgcagatct gcacctcggc gatctcgccg
    12601 tacgcggccg accgcaccga gtggatcagg ttgagtgcgg cgtcgctcga cgggcggtcg
    12661 ctcgaccgct gcgacccgac cagcacgatc ggcacgggcg agtcctgcac catgaaggag
    12721 aggatggcgg ccgtgtgccc catcgtgtcg gtgccgtggc cgatgacgat cccgtcggcg
    12781 cccccctcga tctcctcgcc gatcgcccgc gccagcgcga cgtactgctc cttcgccatg
    12841 ttctccgaga acacgccgaa gagcttcttt gtcgtcaggt tgcagatgtc ggccagctcg
    12901 gggaccgccc cgtacagctc gccgggcgtg aacgcgggga tgacggcgcc cgtcctgtag
    12961 tcgaggcgcg aggcgatcgt gccgccggtc ccgagcagcg tcacgctcgg caggtcgggc
    13021 cgccggggga actccttctc gggaatcttg tagacggcct ccttgtagcc cacctcgctc
    13081 acgcgcgcga tccggtccac gtgcaggccc acgttgtagc cgttcttcag cttgagcacg
    13141 acgtggaggt cgtcgagcgt ctcgctgcgc gggaggatga ccccctcgaa gcggctgccc
    13201 gcgtcgttct ccacctgcac gtcgctccac acgcggacgc cccacgcctc gagcgccgcc
    13261 cgggcccgtc ccttgtatcc cttgagtgcg tcggctgcgg tgctcatcgc cctgccatct
    13321 ccgttcgaat cgcgccggcc acgcgctcgg cgggcacgcg cccccgcagg tcgcccatgg
    13381 cgatccccat cgcgaggcgc gcgagcaggc ccgtatcgtg ccggtgcgcc ctcgcccgcg
    13441 cggccgcgag ggccgccact ggaatctctt cccacccgtc cggcgcgcat ccgaggcgct
    13501 tctccgcgag cagcgccgcc accggcacgc cggggcgctc ggccatcgcg cgaaccacga
    13561 cgtcccacgc ctgccagatc accgcgcttc gcgcggcctc gcggaagaac tcgcaccagc
    13621 gctcgtcggg gatggcgtcg acggccacgc cctcacgcct gagccccttc agtcgctcgc
    13681 cgaacaactc ggccgcccgc cggagcggcg cgccgcagcc gtccaccacc atgtccacca
    13741 gccgggccgc cccgcgccgg atcaggaagt aggccgtctg ccgcggcacc ccggcggccg
    13801 tgtagcgcgc ctcgcgctcc cagggccgcg gggcgagcgc cgcgcgcagc cgctcgacgc
    13861 gctcgcgggt gacccggctc ggcgggctgt cggtgtcggg gtacatccgg tcggggcccg
    13921 ggaggatgcg ctcgaagtcg gtgctgccgt cggggaacgg ctggcgcgtc tcgttcggca
    13981 cgccgtcgag cgcgtcgacg tagcgcagcc ggatctccgc ggccgcggtc agcgtgtcct
    14041 cacccggtcc ccacacgacg gcaatcgcgt cctccgggtt gcagtgcagg tgcgcccgca
    14101 cgcgccgcag ctcctgcagc gagccgtggt agtcgggcca cttctcgccg tggagcagga
    14161 tcgggcgctg gtcgagcccc gcaatgaccc gcacgcgccc ggccagctcg tgggcgaagg
    14221 tcaggtcggg ctgcgtcggc cagcccagcg tgccggtgag gcccttcagc cggacggccc
    14281 gcaccgtgaa cggcccgttg ccgagctcga acccggcgcg ccgcttctcc tcggccacga
    14341 accgctccca gccctcgcgc cgcaggaacg actgctcgga cgaggcgaag agccgggtga
    14401 cgtccgcgct ctcgcacccg atgtcggctg gcgcgctgaa gccgcgccgg atcagctcct
    14461 cccgcaggcg cagcaggttc acctgccgca ccgcctcgcc gtgcacgagc cccggcgccc
    14521 agcccgcctt cggcaccccc ttgatctcga cccggcggcc gccgcgcacc gacacgttca
    14581 cgtcctgccg gctggcgccg atccccaccc gcacgtgccc ggtactgcgg cacacccggc
    14641 cgatcagcag gatcgcctcg gcgacctcgt cgggcgttcg caggtcggga ccggtgacgg
    14701 tttcgatgag cggcatcccg aggcggtcgg tgcgccagac gatgagatgc cccttgtcgg
    14761 acacctcgcg gcacgagtcc tcctcgacgc tcacctgcag gatggacagc tcacggccgc
    14821 gaaacggcag ccgcccgttg acgccgacga tggccgtgcg ctggaagccg gtgggaatcg
    14881 agccgtccag gtactgcttg cgggcgatgt gcacctcgtc cacgatgtcg cagcccagca
    14941 tcaggcactg ctcgatggcg acgtccaccg cctcctggtt caccaggaag ggaggcgtgt
    15001 cgtccatctc gtacgtgcag acgttctcct cgtggagcag gtagatgatg ttcttgcggg
    15061 tcttgaactc catcagtgcc gtgccgtcgt actcccccag ctccgaaagc gtggggcgca
    15121 tgtggcgcag tacctcgccg tcgtgatcgc gcgtgtagcg ccgcgccggg cagtggcaga
    15181 acatcttccg ctcggtcagg agctgctggt gcacctcgag gccggcaatc aagcccacgt
    15241 ccgcgtaatc gatcgactcc accacacgcc ccccggcccg cctccgggcc tcgaacaaaa
    15301 ccgcaagagt gtagcacccg ccttcgctag acgctcgctt cgctcgcagc tccggcgggc
    15361 aggctccgcc ttcgctagac gctcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnncgca gctccggcgg gcaggcgcgg ctacggcggg gcaggttcct tgaaccttga
    15541 gtcctgagcc ccgagccttc ctggggctac catgatccca tggcctcctc cgaccccgag
    15601 ggcccgctgc gggccgacgt cagcctgctc gggacgctgc tcggcgacgt cgtccgcgac
    15661 cgcgagggcg tggaggtctt cgagacggtc gagcaggtgc ggcgcctggc gaaggcggcc
    15721 cgcgccggcg acgagcaggc gttcgagacg ctgcgcgccc gggtgctcgg cacgctcccg
    15781 ctcgacgagg cgctgccggt ggccagggcc ttcgcccact tcctggggct tgccaacatc
    15841 gccgagcagc accaccgcat ccgccggcgc cgccagtacc tcgccgatcc cgccacggcg
    15901 gcgcagcccg gctcgttcga ggaggcgctg ccgcgcctgc tcgccgccgg cgtcacgcca
    15961 gccgacgccc aggcggccct cgaacgcctg tcgatcgggc tggtgctgac ggcgcacccg
    16021 accgaggtgt cccggcggac gatcctcctg aaacagaacc ggctggccgc gctgctcgcc
    16081 gagcgcgacc gccccgacct cacggcctgg gagcgtgacg agaacctcga ctcgctccgg
    16141 cgggtgatcc aggagttgtg ggagaccgac gaggtgcggc gcggccggct gtcgcccctc
    16201 gatgaggtcc ggtggggcct ggcgatcttc gagcagacgc tgtgggacgc gatgccgcgc
    16261 ttcctgcgct cgctcgatcg ggccgccggg cgacacctgg gcgccgcgct cccggtcaca
    16321 gccgcgccga tccggttcgg atcgtggatg ggcggcgacc gcgacggcaa cccgaacgtc
    16381 actcccgagg tgacgtggca aacctgcctg atggcacgct gggtggccgc cgggttgtac
    16441 ctgcgcgagg tggacgccct ccgttcagag ctgtcgatga cggccgccag cgacgagctg
    16501 cgtgcgcagg cccgtggcga gcgcgagccg taccgcgtgg tgttgcgcca gctggcggcg
    16561 cggttgcggg ccacgcaggc atgggccgca gcactgctcg agtacgcgac gcccgggccg
    16621 ccggccgggc cggcgctggc gccgggcgcc gccgccatgg gtgtccccgc ggacgatccg
    16681 ctcctcgagg acgaggagct cacgggcgtg ttccgcctct gttaccgctc gcttgtcgag
    16741 accggcaacg ggccgatcgc gcgcggccgc ctgctcgacg cgtgccggcg cgccgaggtc
    16801 ttcggcctca cccttgcgcg gctcgacgtc cggcaggacg cggcacgcca cgacgaagcg
    16861 atggaggcca ttgccggggg ccagggagcc tggcgcacct gggacgagcg gcggcgcctc
    16921 gacttcctgc ggccgcacct cgccggccct tcgcggctgc gcctcgacgt ggaggcgccc
    16981 cgcgaggtgc gcgaggtgat cgagacgttc cgggtgatgg ctcgcattcc gcgcagctcg
    17041 ctgggcgcgt acgtggtcac catggcaagc cggccgtccg acgtgctggc cgtcctcgcg
    17101 ctgcagcagg cggcaggcgt cgagccgccg cttcgcgtgg tgccgctgct cgagacggga
    17161 cgcgacctcc aggcggccgg tgaggtgatc ggcggacttc tcgcggtgcc ggagtaccgc
    17221 gccgccatcg gcggccggca ggaggtaatg gtcggctact ccgactcgag caaggacgtc
    17281 gggcgcttca gcgccgggtg gaggctctac cgggcgcagg aggagatcgt cgaggcatgc
    17341 cgcctggccg gcgtccgcct gacgatcttc cacgggcgcg gcggcagcgt ggggcgtggc
    17401 ggcggcccca cgcacctggc gatccgtgcc cttccccccg gcgcagtgga cgcgtcgctg
    17461 cgcgtcaccg agcaggggga gatgatccag gcacactggg gcctccccga gctggccgtc
    17521 cggaccatgg aggtctacac cacggcgacg ctcgaggcga cgcttcgtcc cggccgcggc
    17581 ccgactcgcg agtggcgaga gcgcaccgat gcgctggcga aggtggcgcg caacgcctac
    17641 caggcgatcg tccaccagca tcccgccttc gtggagtact tccgcgcggc aacccccgag
    17701 gtggagatcg gccgcctgaa catcggcagc cgccccgccc ggcgttctgc gggaaagggt
    17761 gtcgggtcgc tgcgcgccat accctggcag ttcgcctgga cgcagacgcg gctgatgctg
    17821 gcctcgtggc tcgggctcga ggaagcgctg ggcggcgccg ccgggcccgg cgacggcaag
    17881 ggcgacgggc ttcgcgagat gtaccgggag tggccgtact tccgcgtgct gctcgacctg
    17941 atggagatgg tattggcgaa ggccgatccg gccattgccg ccgagtacga ccgccatctc
    18001 gtgccgccgc acctgcagcc gctcggcgag gagctgcggc ggcgcctcgc gctcgcgatc
    18061 gaccgcgtgc tcgccgcgac ggggcacgcc acgctgctcg aggagaacca cgtcctccgc
    18121 cgctcgattg ccgtccgcaa cccttacgtg gacccgatca atctcctgca ggtcgagctg
    18181 ctctcgcgcc tgcgatccga gtcctcgccg gacgagcgcc tctggaccgc gttcgcggtc
    18241 acggtcaacg ggatcgccgc aggcatgcgg aacacgggat gaacagacag cgtcggggcg
    18301 agcctcgtgg ggatcagaga tcagatcgtg caggaggtgc cggaggtgct ggaggtgcag
    18361 agggtgcagg tgcaggaggt gctggaggtg cagagggtgc aggtgccgga ggtgctggag
    18421 gtgccgaggg tgcaggtgca ggaggtgcag cgccgatggt gctcgaagtg ccggtgctga
    18481 gagtgcggcg cctgcactcc cagcaccagc accggcggca ccttctgcac ctcctgcact
    18541 ttcggcacga cgggatcagg ggattcaccg cagcggcgca accgttgaag gggtcgggag
    18601 taattagaag tcgtgccgag tttccgactt ctgattactt ccgcccctac cgggctcccg
    18661 gcacgacttc taattactcc cgacccctac tgacgaagtc gttccagtgc aggatcgcgt
    18721 tgaagagcat gttgaactcg ccgaggttct gccagcggta gcacgggttg gtggcgaaca
    18781 gcagcacgcg cccgtcgccg agcggcacgt cgatggcggc cggccgctcc ttgatctcgc
    18841 ccgcaccctt catcaggccg ctgagaaccg acgcgtcgcc tccggggaac tgcatcaaga
    18901 cctgcccctt gtcggtgtcg ggaacgcgaa gcagcgggcc cccggcccac cgtaccggca
    18961 gcaccttctt gtcgtagccg tagaagatcg ggtggtcggg gcgaagcagc tccacctgga
    19021 caacggggcc tggcgcgtag aactgcgggc cgggccgcga cgcgtccacg cgccgggcca
    19081 ggccgaactc gggcggaaag tagctggccg cccccatcgt caccagcagc cctcccttct
    19141 cgacgaaggt gcgcagctcc atcacgccct cgagccccat gccgcccgtg atgtcgtccg
    19201 actcgccgta catgccgagg ttcctgaacc gatccgtctt ccggtacgcg agggggcggg
    19261 acttgggctc gagatcgaag accagccgct tcgcgctgcc cgactggctt gggacgatga
    19321 tgagatcgaa ggccgaccgc aggtcgccct gccggacgcg ctccttgaag atcagctcgt
    19381 agggaacctc gaaccgatcg agcgagtagc gaacccaccc cacctcctgg gtgcctcccc
    19441 aagtgctgaa caccgccacg cgcgggaggt ccaccgcgtg ggtggggacg tcgggccgcc
    19501 cgccgagcga cactgccacg aggccgaggc gctcgatggc ttccaccgcc ttccgccgct
    19561 ccgcggcgcc gcgcacctcg atcagcagcg agcctgccgg gagccgcgtg tcgccggccg
    19621 cgaacgcctt ggacgcggcg tgcacgtcca tcgagcgcag ttcgaaccgg agcgagacga
    19681 ggtttgcaga gccgttggcc gccgccgcga tccagccggc gccctcgccc ttgccatcga
    19741 cccgcccttt cgctgtgaag cggtccaccg ggtcggtggg gacggcgagg atcgccttgt
    19801 cggcaaccgg cttcacctcg gtgtgcgtca gcagccccat cgtccagccg ctgtcgtcgt
    19861 aggtccggag gttcgggtcg gggaagtcct gcttctcgag caggatcttc gcgagccgcc
    19921 cgtacggctg gtcgagacga acgacgagcg agcccgccgg gaaggcgagg tcgccgatct
    19981 tgacctcgcc gcgggtgcgt cccagctcga tgccctgcgc gatgaggacg tcggcgacga
    20041 aggcgacgcg cgtcatgtcg gcctggcctg ccggaatcac gaacgcgtag ggcgcgttct
    20101 tcgtgccggc ctcgacggcg ttccagctct tccggtagaa gttctcgagc acgattttcg
    20161 ggaaccgcga cgtgagctgc agcgcgctga gcaccgccgt ctggccgtag ttcacgttgt
    20221 tgcggagcga ccactcgact tccttgtacg gcgggagcgg gcggtaccac tcgcggctgg
    20281 tctgcccgcc gccccggtcg ccgcgatcga tcttgcgcag catcgtgttc gcgccgccgt
    20341 tgccgaacac ttcgtacatg cgcagcatgc cgttgtggtt cgaggccatg aagccgaagt
    20401 agccgggcga ccacatgtcg acgaaggcgt gcgtccagac gccgggcatg ccgtacttga
    20461 tcgcctgcgc catctcgaag ttggcgtacc agggcagctc cgcgtagagg atcggatcga
    20521 gcgtcgggtt ctgtggcgcc tgcccgctga aggtgaaaag gaacggcacc gattcgtgga
    20581 tgtcgtgcag cacgggaggg tgccactcca ggtaccactt caggaccgcg cgcgtgatcg
    20641 cctgcgaata gttgatgtcg cggttgttgt cgtggcggac gtacttgccc cagtacggcg
    20701 gccccccgta gctgtcgtcc tcgcccgttt cgtccacctt gtacgcgtag taccagtcga
    20761 cgtagcggtc gcgcccgtcc gggtcggcca ccggcgtgaa ggtcacgatc acgttgtcgc
    20821 gaatctcgcg atacagcggc gactcgtcgg cagccagccg gtacgcgagc tccatcagca
    20881 tctcgggcgg tcccgtctcg cccgagtgga ggccggccat gacgtggtag attggcttgg
    20941 cctcctcgat tacctggcgc gcttgcgcct cgctcagttg ccgcgggtcg gcgagccgtg
    21001 cgaggcgctc gcggtagctt tcgagcccgg ccatcgtgtc ggccgaggca atcgcgacca
    21061 cgaccagcgg ccgcccctcg tcggaggttc cgatctccat gaccctcacg cgcggcgacg
    21121 cggcagccag cgcgcggtag tacttcagga tgtcctcgta gtacgtcagc ttcttcggct
    21181 cgccgacgtg atgcccgatc acgtccctgg gagaaggaat gccggcggca acgggcaggt
    21241 aatccaccag ggggctgatg aactcgggcc tcgtcgtcca ctcgcggacg aggcgtgcga
    21301 actcgccgtc tgccgcctgc ggggcctgcg cggcaggcag gacgagtccg gccgaagaga
    21361 gggcgagcac caccagcagg gcgagcccga agcgccggga ccgaacggac atgcaaggct
    21421 cctgatttaa cggttgagtt gcgagcggcc ggcgggtatt ctagcagtcc tccggacccg
    21481 ctcggggtta gggcctccca gccatgcgcc tcgccacgct tgccgccgtg ctggtgtcgg
    21541 ggttcctgtc gtcaggctgc ctggtcattg gcctgcaccc gttctacgac gacggcagca
    21601 tcgagttcga cgaggcgctg atcggcgagt gggagagcgg cgaggaatcg ctgcggattg
    21661 cgcgcagccc gtggaagtcg tatcgcatcg actacacctc cggggcagag acagcgtcct
    21721 acgacggaca cctcacgcgg ctcggcagct tcaagctgct cgacctggtg ccggtcacgg
    21781 aaggcgccat ccgggtgcac ctccccgtgc gcgtgaagcc gctgggtgac accctgtcgc
    21841 tggccccgct cgacttctgg tggttcaggc gggaagcgga gcatggccag ccgcaggccc
    21901 tgcggccggc gatcgatgac gaaggcgacg tcctgctgac cgcggcgacc gctgcggttc
    21961 gcgactggct cgcggctcac gccgagcatt tcgacgacga cccggtgcgc ttcacgcgca
    22021 aaccggcccg ataaggtttc ctgaaacggc acgcctcgtg catcatgcgg cttggcgcgg
    22081 cacgacgcct gcccaaagcg ccggaaaacc gggcggcgca agggttgcag cccttcgaat
    22141 ggctctgtta caatcgcgtg tcctgcgctt tttcttctat cccgccagag aggctcccgg
    22201 gggctttcat gcagcagcgc cggcttcggc tcggtgacat cctagacgac tactgcccgc
    22261 gcgagcgccg tatcaccaac cacgcggtcg tcgcgaagat cgacgacgag gtcaggcaga
    22321 cgcggtgtct cacgtgcgac acggaacacg aataccggca ggggaaggcc ccg
//
LOCUS       DAOPQX010000016        22201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_2925_length_22201_cov_4.650817, whole genome shotgun sequence.
ACCESSION   DAOPQX010000016 DAOPQX010000000
VERSION     DAOPQX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 22201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22201
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2925_length_22201_cov_4.650817"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKK95_01780"
     CDS             <1..535
                     /locus_tag="PKK95_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16962.1"
                     /translation="NQAGGAGQGFTWFRANGEYQPSAGVRATFAYNLSRTLSAEAGFT
                     YSASRIRYIVSDDPELSSGFTSPGQRLSEYFVDGAVVVRLKRLAFAHGRGRPFALAGG
                     GYLRQLHAGRALVETGQVYYAGGGVKYLLSPRGRGLVRAFGLRLDARACVRSGGFGFS
                     DGATISPAASAGIVAAF"
     gene            complement(557..1969)
                     /locus_tag="PKK95_01785"
     CDS             complement(557..1969)
                     /locus_tag="PKK95_01785"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOC16963.1"
                     /translation="MKHSSPLGWRAHLHVSALVLLLGVASAAQTPITPPDNKFTPAED
                     VKLGREAAQQVEQELPILRDQQVDAFVDDVGRRLAAAVPPAFRHPEFEYSFTVVNARD
                     INAFALPGGPMFINRGMITAARTEGEVAGVMAHELSHVVLRHGTAQASQATKYQLGQL
                     AGQVIGAIVGGTAGAVIAQGSQFGLGAAFLRFSREYERQADLLGAQIMARAGYDPMQM
                     ATMFETIRRQGGSRGPEWLSSHPNPGNRQEAIEKEARMLQVRDQDRESPNLAAVQARL
                     ERMPRALSTEEIARRGGSGGGGGEVGTTGTLSASVEPPSRDFRTHEVGKLFSVSVPEN
                     WQPVPAGNAVRFAPEGASASSGRRQVFTHGIEFGVAANDSADLEEATQALVSAFAQSN
                     PGLKVAGRAQRVEFGGRQGLRVTLRNASSATGREEAVVLTAALLDGNDLFYSVGVAPV
                     SELGTYRQALARVNDSVRLR"
     gene            complement(2044..2895)
                     /gene="aroF"
                     /locus_tag="PKK95_01790"
     CDS             complement(2044..2895)
                     /gene="aroF"
                     /locus_tag="PKK95_01790"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOC16964.1"
                     /translation="MDAIEERRRVPVVTRPVRVGGAVIGGDAFTVVAGPCAIESRGQF
                     MTVGRHVRDEGAAVLRGGVFKMRTDPHSFQGLGPEALAYADEVRRATGLPFVVEVAAI
                     EQVDVLAPHADALQVGSRNMHNYYLLKELGRTRIPVLLKRGFAAELREWLLAAEYVAA
                     GGNESIVLCERGIRTFEPEMRNTLDLAGAVWARQHSPFPVLVDPSHATGRPELVTPMC
                     LAAAAAGLDGVMVEVHHDNAAALSDGFQALTLEGFSRLTRALRSILGALGRPLSSPVE
                     EGFSPAG"
     gene            2948..4027
                     /locus_tag="PKK95_01795"
     CDS             2948..4027
                     /locus_tag="PKK95_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HOC16965.1"
                     /translation="MMPEADAATAIAFVQIDAQTFAFVEAPLVPAARPVPGRAAFLAP
                     RFSLEGGPWWLTARGDEIRRVSVGEWERLFPATPALHTSPRWLAAEQRRFREGFETLQ
                     ARLADGRLRKGVPITRTRACLDAGQAEALFRRLAARVPALPKGLVAYGLFLPGDRSAG
                     PEFLIGATPEILFDIEEEGRRVRTWAVAGTRRAEPGAGRALAESAKERAEHWEVVDDL
                     LAQARRVGSPVAGATIVRRFGSLLHLVTEIDVRPANLADFEDLVRALHPTPALGVSPR
                     GSEGDSWLRGIDPGGERRRFGAPFGIRWPSGDGRAAVAIRGLQYFQGHLVIWAGCGVV
                     ASSDYAAEWAEALDKLESVRTLWGI"
     gene            4015..5592
                     /gene="menD"
                     /locus_tag="PKK95_01800"
     CDS             4015..5592
                     /gene="menD"
                     /locus_tag="PKK95_01800"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HOC16966.1"
                     /translation="MGNLNQPLALEVIETLQRAGARTVCLCPGGRNAPLVEALEPARH
                     TAFEVLDFFDERGAAFFALGRARRDGQPVAVVTTSGTAAAELLPAMVEAYYQGAPLVA
                     VTADRPWSFRGTGAPQTIEQPNLFGVYARQAIDLDRAGTRWRIDPCCWPVHVNVSFEE
                     PLLDGWDAEASAARESSPPPAAAGATSDAGAAVAAAASIRRPLVILGGLWSDGDREAA
                     QSFCVRMRAPIIAEPSSHLRSRLGGLLLRGRERSAALGLERGWFDGVVRIGDVPSFRL
                     WRDLERRKDLPVVSISRQPWRGLTHGVHVQVARGAPLPLEALAEGAAAGVSEHPELGS
                     ADGRIALAIEKLLKRFPQSEPAIVRRLSETIPAGSFVYLGNSLPIREWNQFASDQEFR
                     YGENRGANGIDGQLSTFLGWAGPGHENWAVVGDLTTLYDLGAPWALRHVEAPLRIVVV
                     NNGGGRIFRRLFRNPRFQHEHTLAFDQWARMWDAAYASDAGLVPERDPVIVELRPDPA
                     ETDRFWEGYKEAGQATM"
     gene            5628..6395
                     /locus_tag="PKK95_01805"
     CDS             5628..6395
                     /locus_tag="PKK95_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC16967.1"
                     /translation="MVRVIALHGFLGRPSDWDVLRSAMPWAEFEAVDLWPLVGGVLAG
                     PPWAEVDAVLEREIRERARREPLAPTIVLGYSFGARLALAVEALALGTLPVAGVCLVS
                     CHPGLGEEDLDTRAVRRVNDEAWALRFETDTEEEIRQAWDGQPVLASSAAAPGRAPWT
                     LPASRRALAVAMRRFSLAGQPDFSERLRRWRTPLLWVTGDDDWKFGDLAAWLHETGAR
                     AEFASCPHAGHRVPWDNPVEFAALVRRWLAGVPGIAA"
     gene            6406..7218
                     /gene="menB"
                     /locus_tag="PKK95_01810"
     CDS             6406..7218
                     /gene="menB"
                     /locus_tag="PKK95_01810"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HOC16968.1"
                     /translation="MWKAVKQYEDILFERTGDGIAKITINRPERRNAFRPRTVTELID
                     AFTICREDAGIGVVILTGAGDKAFCSGGDQKIRGDQGYVDERGVPRLNVLDLQKQIRA
                     LPKPVVAMVAGYAIGGGHVLHLVCDLTIAADNAVFGQTGPKVGSFDGGLGSSYLARVV
                     GQKKAREIWFLCRQYDAQQALEMGLVNAVVPLAELEKVTLEWAREMLQHSPLALRCLK
                     AAMNADCDGQIGLLDLAGNATLLYYMSEEAQEGKKAYLEKRKPNFGKFPRLP"
     gene            7215..8123
                     /gene="menA"
                     /locus_tag="PKK95_01815"
     CDS             7215..8123
                     /gene="menA"
                     /locus_tag="PKK95_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOC16969.1"
                     /translation="MTPPPAPSTSPWLLAARPKTLTATLAPIVVGTGLAVGMSERPAR
                     LALSLVALLSALCIQIGTNLVNDAADFVKGADTAERLGPIRVTQTGLLPARTVMRAAA
                     VFFLAATLLGIPLVLAGGVPILIIGLLSLAGGYAYTTGPLPIAYRGLGEVFVLLFFGF
                     AAVKGMSYVLVGEGLWPWAELAALQVGLQSATLLAVNNARDIAGDRKADKRTLAARFG
                     LTFARAEIAALVALPFVLAPAWFAAGKPGAALLPWGSLPIAVRLVRGVWTEPPGRRFN
                     VFLAWSALLQLLFSALTCAGLVLGGR"
     gene            8123..9376
                     /locus_tag="PKK95_01820"
     CDS             8123..9376
                     /locus_tag="PKK95_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC16970.1"
                     /translation="MPELVAPRPGLDTALAGRIDWKDDAAAALILANPEWVARSGEAA
                     GLARRLLPELAGHVFVATSGTSRGEGAQPRWVALSRRAVRASAAAVNLHLRAEAGDVW
                     AHALPLFHVGGLGILARAQLSGARVEDAAGGSWDPERFLRRAAECRATLGALVPTQVH
                     DLVTAGLKAPRTLRAIVIGGGRMDPALYREARALGWPCLPSYGLTETASQVATAPLES
                     LAETHCPTALPMLAHAEARVDRDGVVWVRGASLLTCYVEIENGRARAWDPRAADGWFR
                     TGDLGVMHAGGLEVRGRGADTVKVLGELVWLPRVEAEAARWLAQEPMLRGMRVDLAVA
                     AVPHPRLGHELVLAVSPAAGGSELPSTDLLLASLRRFQAAALPVERVRRLEVVETIPR
                     TALGKCRRELLASRLSRPAATRDVP"
     gene            complement(9264..9797)
                     /locus_tag="PKK95_01825"
     CDS             complement(9264..9797)
                     /locus_tag="PKK95_01825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOC16971.1"
                     /translation="MPARGSGDFLEVISMRVPVQVHFDEADPRGVLFFGRILTLAHRL
                     FEEHVVPRLVPRWEDWFASEELAVPIRHAEASFSRPMRPGVQHAAEIEVAEVGATSFT
                     VRTRFLEAAEGTRMCAETRVTHVFVEGRTWAKREIPPEIRARLESLRAGTTATPAAPS
                     GISPGPSGESSPPPRAA"
     gene            complement(9781..10404)
                     /gene="udk"
                     /locus_tag="PKK95_01830"
     CDS             complement(9781..10404)
                     /gene="udk"
                     /locus_tag="PKK95_01830"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOC16972.1"
                     /translation="MQRPVVIGVAGGSGSGKTTVVRRIVDSIGQEHVAVLEHDRYYRD
                     HPELRLEERAALNYDHPDSLETDLLVRHIEELKAGRPIELPEYDFTRHARRASSQSAQ
                     PGRIIIVEGILIFADAALRGLMDVKVFVDTDDDARFIRRLQRDVAERGRTMASVVEQY
                     LATVKPMYLEFVEPTKRYADIIIPRGGHNEVAVSMLLTMIRSLAGAR"
     gene            10636..11256
                     /locus_tag="PKK95_01835"
     CDS             10636..11256
                     /locus_tag="PKK95_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16973.1"
                     /translation="MKRSLLVLCLAAAIALPWQAGILGQAAEKKPDNQLPERFRAFVT
                     NLGDVRTTADNRMVEMQISRWTTEEEEQKLFEALKEKGPRAMLSVMQGLPATGWIRSQ
                     GGLSYDLRYARQAAGKEGGRQITLMTDRQMNFWELQSATVSRDYPYTVVRLATNKEGK
                     WEGSMTIATKIELQAGILVFEDLSTRPIQLMSITHEKKNEKNNEKK"
     gene            complement(11340..12317)
                     /gene="pta"
                     /locus_tag="PKK95_01840"
     CDS             complement(11340..12317)
                     /gene="pta"
                     /locus_tag="PKK95_01840"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOC16974.1"
                     /translation="MNPFITDIRTKATSRPVRAALPDAEDARVLRAAAILRDNRLAEP
                     VLVGAEAAIRSLAAANQVDLAGIEIADPASSAWHDEFAALLFEKRKAKGLTMEQADAM
                     ARQPLYFAGLMLDTGRVAVVVGGNVSSTGDVIRAAIHTVGVAPGISIVSSYFIMVLAD
                     GRLLCFADCAVNPDPNDAQLADIAITSARNFQAVTGLEPRVAMLSFSTKGSAEHPHVE
                     KVRQATRLVREREPQLAVDGEMQADAALVPAIGERKCKGSPVAGRANVLVFPDLDAGN
                     IGYKLTERIGGAEAVGPIVQGLRKPYCDLSRGCSVDDIVNVVAICGLMS"
     gene            complement(12437..13684)
                     /locus_tag="PKK95_01845"
     CDS             complement(12437..13684)
                     /locus_tag="PKK95_01845"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOC16975.1"
                     /translation="MNVFVLNCGSSSLKFQIIATDEDQIAKDTDQQLAKGLIERIGSE
                     AIITLQVGNQTPFKGAAPLRDHKAALAYIIKWMSQPETRIPGITSLSDIHAVGHRVVH
                     GAERFKGSILIDDAVIGGLQDCIDLAPLHNPANLKGIFAARELLGPQMPQVAVFDTAF
                     HSTMPEKAYLYAIPYQLYRRFKVRKYGFHGTSHRYIAFRYRKLTGRSRQDTNIITVHL
                     GNGCSACAIKGGKSFDTTMGMTPLEGLMMGTRSGDIDPTVIDYLVHKEGSSFDEIFST
                     LNKRSGLLGVSGLTNDMRELEGEAEEHQDRRAILAIDMFCHRVKKYIGAYLAEMNGAD
                     AICFAGGIGENSSTIRRKVLSDMEWLGVKLDEARNLQAVRGKEMLISADDSRVPVYVI
                     PTNEELILARDTVRAVKSLPFPC"
     gene            13805..15811
                     /locus_tag="PKK95_01850"
     CDS             13805..15811
                     /locus_tag="PKK95_01850"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOC16976.1"
                     /translation="MNGRGWARSLVLVVAAVGVTAVAHAQSAGARAAAGPLRVTSPGG
                     ALEVTVATDGVLTWSAAHRGRTILRPSRLALTLGDGRVLGTAPKVTATSVRSIDQALT
                     PPVAYRRARVADRCQERRIDFAGDFSLVVRAYDDGVAYRFVTRLPGEITVRDEDATLV
                     FEGDHLLYFPEEESFQSHQEREYKRLRISEIGPTRFSSLPALVVIEGGPKVAITESDL
                     FDYPGMDLKSGSEAHTLRGLFPAYPRKVRIERDRDKVVVEREDYIAKTRGTREFPWRV
                     LVVAERDETLLDSDIVFRLASESRIADTSWIRPGKVAWDWWNANNVYGVPFRAGINTD
                     TYKHYIDFAAEHGIPYVILDEGWYKLGDLMAAAPELDMPALAAHAKQRNVGLVMWVVW
                     KTLDQQLQPALDQFARWGVKGIKVDFMQREDQWMVNFYERVAREAAARHLLVDFHGAY
                     KPTGLYRTWPNVVTSEGVLGLEQSKWGPAASPDNAVTFPFMRMLAGPVDYTPGAMLNA
                     TKADFRPVFNRPMSQGTRAHQLAMYVVFESPLQMLADSPSNYRKEPDSLAFLSAVPVV
                     WDETRILSAKVGEHILVARRSGREWYVGALTSWEPRDIEVDLGFLGAGSFTADIHRDG
                     PNADRAGVDYVREQRPVTSADRLRVHLAPGGGLAVRIVGAVPKA"
     gene            16024..16461
                     /locus_tag="PKK95_01855"
     CDS             16024..16461
                     /locus_tag="PKK95_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HOC16977.1"
                     /translation="MASFERHSDVTWEGSIREGKGEAVAGTGAFTLPVTFPSRIEDPA
                     GRTSPEELIAAAHAACYAMALTAALGRRNASASRLKVTSTVRAELAQGITITASHLKV
                     VAEGLQGISAADFPQFAREAEQGCPVSNALRGSLKIDVDAQAV"
     gene            16466..17902
                     /locus_tag="PKK95_01860"
     CDS             16466..17902
                     /locus_tag="PKK95_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOC16978.1"
                     /translation="MASLLDRAGRPVPDAALAEFARGLRGATLSPGDPGYDEARRVFN
                     AMVDRRPGLVVQPLDAAGIQHAVRFARRHDVLVSIKGGGHSAPGHAVCNDGLLIDLCR
                     MRAVRVDPEARTAVAEGGATWGEFDAATQGHGLAVTGGRIRGTGIGGLTLGSGSGWLE
                     RKFGYTVDSLVAADVVLASGDLVRASDHEHQDLFWGLRGGGGNYGIVVRFEYRLHALG
                     PLVWGGMLLFPRDGREREVLRAYRDFMEAAPDEVGGAAALVTVPPLPMIPEAARGKAA
                     LAIIAVYLGHPGEAEAAFEPMLRLQPAAALVQPMPYLAVQGLLDEANQPGKRNYWKAD
                     TCPSLPDAALDALLGATARPASPLTTVLLQPMGGAAARVPDDATAMGWRQAKWSLHVL
                     GMWEDASDDARQVSWVRAVTGAMAPWAQKATYLNYLMDEGEGRVRDSFGPHYAQMVAL
                     KDKYDPTNFFCMNQNIKPTAHLGGVAGM"
     gene            complement(17955..18650)
                     /locus_tag="PKK95_01865"
     CDS             complement(17955..18650)
                     /locus_tag="PKK95_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16979.1"
                     /translation="MTRISPFAATALLAATALLAACGGKSEEPAQPEPETSAAAEPAV
                     AASPETAAAPAARVEITEALVTGYMEYQKEAYRLVKEYAEKSRKNLESAKGDTSKALQ
                     QVGIAQEYSKELDDKLAAKRKALGLTPEQFDAVREAAQLTANLRLLFNQMGGNQQLEQ
                     MAAAQKASIAALPEDQRAAATAEAEKVMKTFTDGRDLAEVRQKYGDQAAEVLLKHADA
                     LAAQQLQILQLGK"
     gene            complement(18705..19412)
                     /locus_tag="PKK95_01870"
     CDS             complement(18705..19412)
                     /locus_tag="PKK95_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOC16980.1"
                     /translation="MYWIGTSGYNYPEWRGTFYPERFPSAKMLPFYAERFRSVEINYT
                     FYRMPTASLLSGWAGATPDGFRFTLKAPQRITHVARLRGCAQDVETFWRAAAALGPKL
                     GALLFQLPPAFKKDVRVLHDFLRLLSPAMRPAFEFRHPSWHDVESFDCLRSHGAALCI
                     ADSERLSTPVEMTAPHAYFRLRDEGYQDADIVRWAGAIRDAAGGCDDVFVYFKHEEQG
                     LGPAFAARLRGLLEPPQ"
     gene            19521..19943
                     /locus_tag="PKK95_01875"
     CDS             19521..19943
                     /locus_tag="PKK95_01875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOC16981.1"
                     /translation="MRPLRLFALAALVAALAACGDSPTAPSSGVFTQTDLVVGTGADA
                     TAGRNLTVHYTGWLWDPSKADAKGLQFETSRTGQPLVFTLGAGQLIAGFDQGLVGMKV
                     GGLRRLIIPPSMAYSSNRNGPIPPNATLVFEIELLEVE"
     gene            complement(19971..21047)
                     /locus_tag="PKK95_01880"
     CDS             complement(19971..21047)
                     /locus_tag="PKK95_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOC16982.1"
                     /translation="MSRFWRRAALAAALNVTLAIGVAGAQTVVVKNAPAGAAIELRWN
                     GEPLGRASAGADGIARLAFPPEKLGRPETDARVLVDVCEAVRSITLVERTMQPPPPET
                     GCTRTEVGQFVLLQSSTSLLVDLAPAAPTLWVRQGPVPPDWLLNIEAEPAERPRRPAP
                     KGLMLFGDGSFTNFSRFTQSACGTLDCDSSAFKPGYSVGASIWLSQYFGIEGGFLKPS
                     SAKATGEESTYSYESTLDAQVITAALLVGVPVGPVRFYARGGATHHRATFHTTQTIET
                     RTLTIDGVQQTIPGGTQELELRTAGWGWLFGGGLEGWLSRRVALYGEANYGSLKGGAR
                     DDGEGELDERISTIRFGLRVRLGG"
     gene            21196..>22201
                     /locus_tag="PKK95_01885"
     CDS             21196..>22201
                     /locus_tag="PKK95_01885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC16983.1"
                     /translation="MDRVGRYQLLDKLGEGGMGVVYKAYDPLIQRVVAVKLISSARTD
                     DPVLRERFFAEARAAGHLSHRNIVTVYDLGEENERPYFAMEFLEGEDLGRMIRRGAPP
                     TLIDKIDLMAQAGRGLAYAHARGVIHRDVKPANIFVTTTGDVKVLDFGLARLARPVGT
                     DLTRTRPFVGTVSYMAPEQVRGEPADARTDLFAFGVVLYEMLSGGRPAFETDSFASTM
                     HRILTEEPEPLAGLDATLPEELTRVVERALAKSRDERYQRVDEMLADLAAFRASLNAG
                     AEPHARFTPTPGPRRASLEGLDVTVGHEAAVDVRTPPPRALPASPVPASPSPTPVSSV
                     P"
BASE COUNT         3178 a         7608 c         7997 g         3418 t
ORIGIN      
        1 gaaccaggcg ggcggagcag ggcagggatt cacgtggttt cgggcgaacg gtgagtacca
       61 gccctcggcc ggcgtgcgtg ccaccttcgc gtacaacctt tcgcgcaccc tctcggcgga
      121 ggccggcttc acctactctg cctcgcgcat ccggtacatc gtgtccgacg acccggaact
      181 ctcctcgggg ttcacgtctc ccgggcagcg cctctcggag tacttcgtgg acggcgcggt
      241 cgtggtgcgc ctgaaacggc tggccttcgc gcacggccgg gggcggccgt tcgcgctggc
      301 gggcggcggc tacctgcggc agctgcacgc cggccgcgcg ctggttgaaa ccgggcaggt
      361 gtactacgcc gggggcgggg tgaaatacct gctctcgccg cggggacgcg ggctcgtgcg
      421 tgcgttcggc ctgaggctcg acgcaagggc ctgcgtgcgc tcggggggct tcgggttcag
      481 cgatggcgcc acgatctcgc ccgccgcatc cgccggaatc gtggcggcct tctgagagcg
      541 ccgccccctc aacgagctac cgcaagcgaa ccgagtcgtt cacccgcgcg agggcctggc
      601 ggtaggtgcc aagctctgag acgggcgcga cgccgacgct gtaaaagagg tcgttgccgt
      661 cgagcagcgc ggccgtcagt acgaccgcct cctcgcggcc ggttgcgctc gaggcgttgc
      721 gaagcgtcac gcggaggccc tgccgtcccc cgaactcgac gcgctgcgcg cggccagcca
      781 ccttgaggcc cggattgctc tgtgcgaagg ccgataccag ggcctgggtg gcttcttcga
      841 gatcggcgct gtcgttggcg gcgacgccga actcgatccc gtgcgtgaac acctgccggc
      901 ggcccgacga ggccgacgct ccctcggggg cgaagcgcac cgcgttgccc gcgggaacgg
      961 gctgccagtt ctcggggacg ctgacgctga acagcttgcc gacctcgtgc gtgcggaagt
     1021 cgcgcgaggg cggctcgacg ctggccgaga gggtgcctgt ggtacccacc tcgcctccgc
     1081 cccctcccga gccgccacgc cgggcgattt cctcggtcga gagggcacgc ggcatccgct
     1141 cgaggcgcgc ctgcacggct gcgaggttgg ggctttcccg gtcctggtcc cgcacctgca
     1201 gcatccgggc ttccttctcg atcgcctcct ggcgattgcc tggattgggg tggctgctca
     1261 accactcggg cccgcgcgat ccaccctgcc tccggatggt ctcgaacatg gtggccatct
     1321 gcatcgggtc gtagcccgcg cgggccatga tctgcgcgcc gagcaggtcg gcctgccgct
     1381 cgtactcgcg gctgaagcgg aggaacgccg ctccgagtcc gaactggctt ccctgcgcga
     1441 tcacggcgcc ggcggtgccg ccgacgatcg cgccgatcac ctggccggcc agctgcccga
     1501 gctggtactt cgtggcctgg ctcgcctgcg ccgtcccgtg gcgaaggacg acgtgactca
     1561 actcgtgcgc catgacgccg gcgacctcgc cctcggtgcg cgccgccgtg atcatgccgc
     1621 ggttgatgaa catcggcccc ccggggagag cgaaggcgtt gatgtcgcgc gcgttgacga
     1681 ccgtgaagct gtactcgaac tcggggtggc ggaaggccgg cgggacggcc gcggccaggc
     1741 ggcgcccgac gtcgtcgacg aaggcatcga cctgctgatc gcgaaggatt ggcagttcct
     1801 gctcgacctg ctgcgccgcc tcgcggccaa gcttcacgtc ttcggcaggc gtgaacttgt
     1861 tgtcgggcgg ggtgatcggc gtctgtgctg ccgacgcgac gccaagcagc aggacgaggg
     1921 ccgagacatg caggtgcgcg cgccatccga gggggctcga gtgcttcatg cccccggtag
     1981 tgcaaggttc gtgacagcgg tcaggagtag cgcaggcctt agccctgccg aacggccgtc
     2041 aacctatcca gcagggctga agccctcctc gacgggcgat gacagggggc ggccgagcgc
     2101 gccgaggatg gaccggagcg cacgggtgag gcgcgagaaa ccctcgagtg tgagggcctg
     2161 gaacccgtcg ctaagggccg cggcgttgtc gtggtgcacc tcgaccatga cgccgtcgag
     2221 gccggcggcc gcggcggcca ggcacatcgg tgtcaccaac tcgggtcgcc cggtggcgtg
     2281 cgaggggtcc accagcacgg ggaagggcga gtgctggcgc gcccagacgg cgccggccag
     2341 gtcgagcgtg ttccgcatct ccggctcgaa ggtacggatg ccgcgctcgc agaggacaat
     2401 cgactcgttt ccgccagccg ccacgtactc ggccgcgagc agccattcgc gcagctcggc
     2461 cgcgaagccc cgcttgagga gcaccgggat gcgcgtccgc cccagctcct tcagcaggta
     2521 gtagttgtgc atgttgcgcg agccgacctg cagcgcgtcg gcgtgcggcg cgagcacgtc
     2581 cacctgctcg atcgcggcga cttccaccac gaacggcaag ccggtggccc ggcgcacctc
     2641 gtcggcgtag gcgagcgcct ccggccccag gccctggaag ctgtgcgggt cggtcctcat
     2701 cttgaagacg ccgccccgca gcaccgcggc gccctcgtct cgcacgtggc gcccgaccgt
     2761 catgaactgg ccgcgcgact cgatcgcgca gggcccggcc acgacggtga aggcgtcacc
     2821 gccgatcacc gcgccgccca cacggacggg gcgtgtcacc acgggcaccc gccggcgttc
     2881 ttcaatggca tccaacccat catggtaacg gctttggccg cgccctgtcg atataatcac
     2941 caccgggatg atgcccgaag ccgacgccgc caccgccatc gcgttcgtgc agatcgatgc
     3001 gcagacgttc gccttcgtgg aggcgccgct cgttcccgcg gcgaggcccg tccccggccg
     3061 cgccgcgttt ctcgcgcccc gcttctcgct cgaaggcggg ccctggtggc tcaccgctcg
     3121 cggcgacgag atcaggcgcg tgagcgtggg ggagtgggaa cggctcttcc cggcaacccc
     3181 ggcgctgcac acatcgccac ggtggctcgc ggccgagcag cggcgttttc gcgagggttt
     3241 tgaaacgctc caggcgcggc ttgccgacgg ccgtcttcgc aagggggtac cgatcacgag
     3301 gacgcgcgcc tgcctggatg ccggccaggc ggaggcgctg ttccgccgcc ttgccgcacg
     3361 tgtgccggcc ctgcccaaag ggctggtcgc ctacggcctc ttcctgcccg gtgaccgatc
     3421 ggcaggcccc gagttcctga tcggcgcgac gccggagatc ctcttcgaca tcgaggagga
     3481 aggcagacgc gtccggacgt gggcggtggc agggacgcgg cgggcggagc cgggtgccgg
     3541 gcgggcgctg gccgagagcg ccaaggagcg ggcagagcat tgggaagtgg tggacgacct
     3601 gctggcgcag gcgcggcggg tcggcagccc ggtcgcgggg gcgaccatcg tgcgacggtt
     3661 cggcagcctg ctgcacctgg tgaccgagat cgacgtgagg ccggcgaacc ttgccgactt
     3721 cgaagacctg gtgcgcgcgc tgcacccgac ccccgcgctg ggcgtgtcgc cgcggggaag
     3781 cgagggggac tcctggctgc gagggatcga ccccgggggc gagcgccgcc ggttcggcgc
     3841 gcccttcggg atccgctggc cctccggcga cgggcgcgcg gccgtcgcga ttcgcggctt
     3901 gcagtacttc caggggcacc tcgtgatctg ggcggggtgc ggcgtggtgg cctcgagcga
     3961 ctatgccgcc gagtgggccg aggcgctcga caagctggag tcggtgagga cgctgtgggg
     4021 aatctgaacc agccgctggc gctcgaggtg atcgagacgc tgcaacgcgc cggggcgcgg
     4081 acggtctgct tgtgtccggg cggccgcaac gcccccttgg tcgaggcgct ggagccggcg
     4141 cggcacacgg cgttcgaagt cctcgacttc ttcgacgagc gcggcgccgc gttcttcgcc
     4201 ctcgggcggg cgaggcgcga cgggcagccc gtcgcggtgg tcaccacctc gggcacggca
     4261 gccgccgagc tgctgcccgc gatggtcgaa gcgtactacc agggtgcacc gctggttgcc
     4321 gtcaccgccg accgcccgtg gtcgttccgc gggacgggcg cgcctcagac aatcgagcag
     4381 cccaacctgt tcggggtcta cgctcgccag gcgatcgacc tcgatcgcgc ggggacgcgc
     4441 tggcgcatcg acccctgctg ctggccggta cacgtcaacg tgagcttcga ggagccgctc
     4501 ctcgacggat gggacgcgga ggcctcggct gcacgcgaat cctcgcctcc tcctgccgcc
     4561 gcgggggcca cgtccgacgc cggggcggcc gtcgccgccg cggcctcgat tcgaaggcct
     4621 ctcgtgatcc tcggcgggct ctggagtgac ggcgaccgcg aggccgccca gtcgttctgc
     4681 gtgcggatgc gcgcgcccat catcgccgaa cccagctctc acttgcgcag ccgcctcggc
     4741 ggcctcctcc tcagggggcg tgaaaggtcg gcggccctgg gtctcgagcg aggctggttc
     4801 gacggtgtcg tccggatcgg tgacgtgccc tcgttccgac tctggcgaga cctggagcgt
     4861 cgaaaggacc ttcccgtggt gtcgatctcg cgccagccgt ggcgcggcct gacgcacggt
     4921 gtgcacgtgc aggtcgcgcg gggcgcaccg cttccgctcg aggcgctcgc cgagggcgct
     4981 gccgccgggg tgagcgagca tcccgagctc ggctctgcgg acggccggat cgcgctggcc
     5041 atcgagaagc tcctgaagcg cttcccgcag tcggaaccgg cgatcgtccg ccggctctcg
     5101 gagaccatcc cggccgggtc gttcgtgtat ctcgggaaca gcctgccgat ccgcgagtgg
     5161 aaccagttcg cctcggacca ggagttccgc tacggtgaga accggggcgc caacgggatc
     5221 gacggccagc tctccacctt cctgggctgg gccggtcccg ggcatgagaa ctgggccgtc
     5281 gtcggcgacc tgaccaccct ctacgatctc ggggcgccct gggcgctgcg gcacgtcgag
     5341 gccccgctgc gaatcgtggt cgtcaacaac ggaggggggc gaatcttccg ccgcctgttc
     5401 cgcaacccgc gcttccagca cgagcacacg ctcgcgttcg atcagtgggc gaggatgtgg
     5461 gacgcggcct acgccagcga cgccggcctc gtgcccgagc gcgacccggt gatcgtggag
     5521 ttgaggcccg acccggcgga gaccgatcgg ttctgggagg ggtacaagga agcgggacag
     5581 gcgacaatgt gacaggcgcc gcacgagcgg ccttgggagg gttggtcgtg gttcgggtga
     5641 tcgcgttgca tgggttcctt ggacggccgt ccgactggga cgtgctgcgc tcggccatgc
     5701 cgtgggcaga gttcgaggcg gtggatctct ggccgctggt tggcggggtc ctggcgggtc
     5761 ctccctgggc ggaggtggac gcggtgctcg agcgcgagat tcgcgaacgc gcacggcgcg
     5821 agccgctggc ccccaccatc gtgctcgggt actcgttcgg cgcccgcctc gcgctcgcgg
     5881 tcgaggccct cgcgctcggc accctgccgg tggctggcgt ctgcctcgtg tcgtgccacc
     5941 cgggcctggg cgaggaagac ctcgatacgc gggcggtccg ccgcgtcaac gacgaggcgt
     6001 gggcgctgcg gttcgagacc gataccgagg aggagatccg gcaggcgtgg gacggccagc
     6061 ccgtgctcgc gtcgagtgca gctgctcccg ggcgcgcgcc ctggacgctg cccgcgtcgc
     6121 ggagggctct cgcggtcgcg atgcggcgct tctcgctggc cgggcagccc gacttctcgg
     6181 agcgcctgcg gcgctggcgg acgccgctgc tgtgggtgac gggcgacgac gactggaagt
     6241 tcggagacct ggcggcctgg ctgcacgaga ccggcgcgcg cgctgaattc gcttcctgtc
     6301 cgcacgccgg gcaccgcgtg ccctgggaca accccgtgga gttcgccgcc ctcgtccggc
     6361 gctggctggc cggcgttccg ggcatcgccg cgtgaggaga cacccatgtg gaaggccgtc
     6421 aagcagtacg aggacatcct gttcgagcgc accggagacg ggatcgcgaa gatcaccatc
     6481 aaccggcccg agcgccgcaa cgccttccgg ccgagaaccg tcaccgagct gattgacgcg
     6541 ttcacgatct gccgcgagga cgccgggatc ggtgtcgtga ttctgacagg cgcgggggac
     6601 aaggcgttct gttcgggggg cgaccagaag atccgcggcg accagggcta cgtcgacgag
     6661 cggggcgttc cccgcctgaa cgtgctcgac ctgcagaagc agatccgcgc gctgcccaag
     6721 ccggtggtcg cgatggtggc cggctacgcg attggcggcg gccacgtgct gcacctggtg
     6781 tgcgacctga cgatcgcggc cgacaacgcc gtcttcggcc agacgggtcc caaggtcggg
     6841 tcgttcgacg gcggtctcgg ctcgagctac ctggcacgcg tggtggggca gaagaaggcg
     6901 cgcgagatct ggttcctctg ccgccagtac gacgcgcagc aggcgctgga gatggggctg
     6961 gtgaacgccg tcgtgccgct cgccgagctg gagaaggtca cgctcgagtg ggcccgcgag
     7021 atgctgcagc actcgccgct ggcgctgcgc tgcttgaagg cggcgatgaa cgcggactgc
     7081 gacggccaga tcggcctgct cgacctcgcg ggcaacgcca cgctcctcta ctacatgtcc
     7141 gaggaggcgc aggaaggcaa gaaggcctac ctcgagaagc gcaagccgaa cttcggcaag
     7201 ttcccgcgcc tgccgtgacg ccgccgcccg ctccgtccac gtcgccctgg ctgctggccg
     7261 cgcgcccgaa gacgctgacc gccaccctcg ccccgatcgt cgtcggcacg gggctggcgg
     7321 tcgggatgtc ggagcgtccg gcgcggctcg cgctgtcgct cgttgcgctg ctgtcggcgc
     7381 tgtgcatcca gatcgggacg aacctggtca acgacgcggc cgacttcgtg aagggcgccg
     7441 acacggccga gcgcctcggg ccgatccgcg tgacgcagac gggcctgctg cccgcccgca
     7501 ccgtcatgcg cgccgcggcg gtgttcttcc tcgccgccac gctgctcggg atcccgctcg
     7561 tgctcgccgg cggggtcccg atcctgatca tcggcctcct gtcgctggcg gggggctacg
     7621 cctacacgac cggccctctc ccgatcgcgt accgcggcct gggtgaggtg ttcgtgctcc
     7681 tcttcttcgg cttcgccgcg gtgaagggga tgtcgtacgt gctcgtgggc gagggcctgt
     7741 ggccgtgggc cgagctggcg gcgctgcagg tgggccttca gagcgcgacg ctgctggccg
     7801 tgaacaacgc ccgcgacatc gccggcgatc gcaaggccga caagcgaacg ctcgccgcgc
     7861 gcttcggcct gacgttcgcg cgcgccgaga tcgccgcgct ggtggcgctg ccgttcgtcc
     7921 tcgcgccggc atggttcgcg gcggggaagc cgggggccgc gctgcttccc tggggcagcc
     7981 tgccaattgc cgtccgcctc gtgcgcggag tgtggacgga gccgccggga cggcggttca
     8041 acgtgttcct cgcgtggagc gcgctgctgc agttgctgtt ctccgcgctc acctgcgccg
     8101 ggctggtgct cgggggacgc tgatgcccga gctggtggcg ccccgccccg ggctcgacac
     8161 cgcccttgcc gggcgaatcg actggaaaga cgacgccgcg gccgcgttga tcctcgcgaa
     8221 cccggagtgg gtggcacgaa gcggcgaggc ggccgggctg gctcgccggc tcctgccgga
     8281 gctggcgggg cacgtcttcg tcgcgacttc cggcacgagc cggggtgagg gggcgcagcc
     8341 gcgctgggtc gcgctgtcgc gccgggccgt gcgcgcctcc gccgctgcgg tcaacctgca
     8401 cctgcgagcc gaagccggcg acgtgtgggc gcacgcgctg cccctcttcc acgtgggcgg
     8461 gctgggaatc ctcgcgcgcg cgcagctttc gggggcccgt gtcgaggacg cggccggcgg
     8521 ctcgtgggac ccggaacggt ttctccggcg cgctgccgag tgtcgcgcca cgctcggcgc
     8581 cctggtgccg acgcaggtcc acgacctggt gacggccggt ctgaaggcgc ctcgaaccct
     8641 tcgggcgatc gtcatcggcg gcggccggat ggacccggcc ctgtatcgcg aggcgcgcgc
     8701 gctcggttgg ccgtgcctgc cgagctacgg cctgacagag accgcctcac aggtggcgac
     8761 ggccccgctc gagagcctgg cggagacgca ctgtcccacg gcgctgccga tgctggccca
     8821 tgccgaggcg cgcgtcgacc gggatggcgt cgtctgggtg cgcggggcat ccctgctgac
     8881 ctgttacgtg gagatcgaga acggccgcgc gagggcatgg gacccgcggg cggccgacgg
     8941 ctggtttcgc acgggcgacc tcggcgtgat gcacgccggc ggcctcgagg tccgcggccg
     9001 cggcgccgac acggtgaagg tgctgggcga actggtctgg ctgccccgcg tggaggcgga
     9061 ggcggcgcgc tggcttgccc aggagccgat gctgcggggg atgcgggttg acctggccgt
     9121 ggctgcggtg ccgcatccgc gtctgggcca cgaactcgtg ctggcggtct ccccggcggc
     9181 cggcggcagc gagctgccct cgacagatct gctgctcgcg tccctgcgcc gcttccaggc
     9241 agccgcgctc ccggtggagc gggtcaggcg gctcgaggtg gtggagacga ttccccggac
     9301 ggccctgggg aaatgccgga gggagctgct ggcgtcgcgg ttgtcccggc ccgcagcgac
     9361 tcgagacgtg ccctgatctc gggcggaatc tcgcgcttgg cccacgtgcg cccctccacg
     9421 aagacgtgcg tcacgcgcgt ctcggcgcac atgcgcgtcc cttcggccgc ctccaggaac
     9481 cgggtgcgca ccgtgaagga ggttgcgccg acctcggcaa cctctatctc ggcggcgtgc
     9541 tgcacgcccg ggcgcatggg ccgcgagaag ctcgcctcgg cgtgacggat cggcaccgca
     9601 agctcctccg acgcgaacca gtcctcccag cgtggcacga ggcggggcac cacgtgctcc
     9661 tcgaacaacc ggtgcgccag ggtgaggatc cgcccgaaga agaggacccc ccgcgggtct
     9721 gcttcgtcga aatgcacctg gacgggcacg cgcatcgaga tcacctccag gaagtcgccg
     9781 ctacctcgcg ccggcaaggc tccgtatcat cgtcagcagc atgctcaccg ccacctcgtt
     9841 gtggccgccg cgcgggatga tgatgtccgc atagcgcttg gtgggctcga cgaactccag
     9901 gtacatcggc ttgacggtgg ccaggtactg ctcgaccacc gaggccatgg tccgtccccg
     9961 ctcggcgacg tcgcgctgca gccgccggat gaagcgggcg tcgtcgtcgg tgtccacgaa
    10021 caccttgacg tccatcaggc ctcgcagggc cgcgtcggcg aagatcagga tgccctcgac
    10081 gatgatgatc ctgccgggct gggccgactg gctcgaagcc ctgcgggcgt gccgggtgaa
    10141 gtcgtactcg ggaagctcga tcggccgccc agctttcagc tcttcgatgt ggcgaacgag
    10201 caggtcggtc tcgagcgagt cgggatggtc gtagttgagc gcggcccgct cctcgagccg
    10261 cagctccggg tggtcgcggt agtagcggtc gtgctcgagg acggccacgt gctcctggcc
    10321 gatgctgtcg acgattcgcc gcacgacggt cgtcttgccc gaccccgacc cgcccgccac
    10381 gccgatcacc actggacgct gcatggtgca ccaatccccg ttcagacgat tccaggcaag
    10441 aggatgaccc ggcccagccg atccgatccc gatcctcgcc gtgtccccgc tggcaacggc
    10501 ccccggtccg aaaccggcgg gtcgggccgc attttcaggc gcagttcagc tacccttgta
    10561 ctggcgcccg ttttgctcgg caaggatagc agggtacggg ccgtccggat tcactcgcga
    10621 gaaaggcggt gaagcgtgaa gcggagcctc ttggtgctgt gcctggcggc ggctatcgcc
    10681 ctgccatggc aggcgggcat cctcggacag gcggcggaga agaagccgga caaccagttg
    10741 cccgagcgtt tccgcgcctt cgtgaccaat ctcggcgacg tccggaccac ggcggacaac
    10801 aggatggtgg agatgcagat ctcccggtgg acgaccgagg aggaagagca gaagctgttc
    10861 gaggcgctca aggagaaggg gccccgggcg atgctctcgg tcatgcaggg cctgccggct
    10921 accgggtgga tccggtcgca gggcggtctt tcgtacgacc tccgctacgc caggcaggcg
    10981 gcgggcaagg aggggggccg gcagatcacg ctgatgacgg accggcagat gaacttctgg
    11041 gagctgcaga gcgcgaccgt cagccgcgac tacccgtaca cggtcgtccg gctcgcgacc
    11101 aacaaggaag gcaagtggga aggctcgatg acgatcgcga ccaagatcga gctgcaggcg
    11161 gggatcctgg tgttcgagga cctttccacg cggccgatcc agctgatgtc catcacccac
    11221 gagaagaaga acgagaagaa caacgagaag aagtaggaat gacgccggct cccccgtgac
    11281 ggcaccgggg agccgggcgc tctcgctctc ctacgttttc cgtccccgtc ttcggtccgt
    11341 caagacatca ggccgcagat ggcgacgacg ttgacgatgt cgtccacgct gcagccgcgc
    11401 gacaggtcgc agtaaggctt cctcaggccc tggacgatcg ggccgacggc ctcggcgccg
    11461 ccgatccgct cggtcagctt gtacccgatg ttcccggcat cgaggtccgg gaacaccagc
    11521 acgttggccc gcccggcgac gggcgagccc ttgcacttcc gctcgccgat ggccggcacc
    11581 agggcggcgt cggcctgcat ctccccatcc accgccagct gcggctcgcg ctcgcgcacc
    11641 aggcgggtgg cctgccgcac cttctccacg tgcgggtgct ccgcgctccc cttggtcgag
    11701 aacgacagca tcgccacgcg cggctcgagg cccgtgaccg cctggaagtt cctggccgag
    11761 gtgatggcga tgtcggccag ctgggcgtcg ttcgggtcgg ggttcaccgc gcagtcggcg
    11821 aagcagagca gccggccgtc ggccagcacc atgatgaagt agctgctgac gatcgagatg
    11881 cccggcgcga cgcccacggt gtggatggcc gccctgatga cgtcgccggt ggaggagacg
    11941 ttgccgccga cgaccacggc gacacggccc gtgtcgagca tcaggccggc gaagtagagc
    12001 ggctggcgcg ccatcgcgtc ggcctgctcc atggtcagcc ccttggcctt gcgcttctcg
    12061 aacaggagcg cggcgaactc gtcgtgccag gcgctggagg ccgggtcggc gatctcaatg
    12121 cccgccaggt cgacctggtt ggccgcggcg agcgagcgga tggcggcctc ggcgccgacc
    12181 aggaccggct cggccagccg gttgtcgcgg aggatggccg cggcgcgcag cacgcgcgcg
    12241 tcctcggcgt cgggcagcgc cgcgcggacg ggccgcgagg tcgccttggt gcggatgtcg
    12301 gtgataaacg ggttcataga gaaggaatca ggaatcagga atcgggagtc aggatccggg
    12361 atcgggaatc ggtcgggaag cgggacccag gggttgggca cccccgaatc ccgaatcctg
    12421 aatccggaat cccgcttcag cacgggaacg gcagcgactt gaccgcgcga acggtgtcgc
    12481 gggcgaggat cagctcctcg ttggtcggga tcacgtacac gggcacgcgg ctgtcgtccg
    12541 cgctgatcag catctccttg ccgcggaccg cctgcaggtt gcgcgcctcg tccagcttca
    12601 cccccagcca ctccatgtcc gagagcacct tgcggcgaat cgtggacgag ttctcgccga
    12661 tgcccccggc gaagcagatc gcgtcggcgc cgttcatctc ggccaggtag gcgccgatgt
    12721 acttcttcac ccggtggcag aacatgtcga tcgccaggat ggcacggcgg tcctggtgct
    12781 cctcggcctc gccctccagc tcgcgcatgt cgttggtcag gccggagacg ccgagcagcc
    12841 ccgagcgctt gttgagcgtg gagaagatct cgtcgaagct gctgccctcc ttgtgcacga
    12901 ggtagtcgat cacggtgggg tcgatgtcgc ccgagcgggt tcccatcatg agcccttcga
    12961 gcggggtcat ccccatcgtg gtgtcgaacg acttcccgcc cttgatcgcg cacgccgaac
    13021 agccgttgcc caggtgcacc gtgatgatgt tggtgtcctg ccgcgacctg ccggtcagct
    13081 tgcggtagcg aaacgcgatg tagcggtgcg aggtgccgtg gaaaccgtac ttgcggacct
    13141 tgaagcggcg gtagagctgg taggggatgg cgtagagata cgccttctcc ggcatcgtcg
    13201 agtggaaggc cgtgtcgaaa actgccacct gtggcatctg cgggcccaac agctcgcgcg
    13261 cggcgaagat gcccttgagg ttggcggggt tgtgcaacgg cgccaggtcg atgcaatcct
    13321 gcaaaccgcc gatcacggcg tcgtcgatca gaatcgagcc cttgaagcgc tccgccccgt
    13381 ggacgacccg gtggccgacc gcgtggatgt cgctcaggct ggtgatgccc gggatgcgcg
    13441 tctcgggctg cgacatccac ttgatgatgt aggccagggc ggccttgtgg tcgcgcagcg
    13501 gcgcggcgcc cttgaagggc gtctggttgc cgacctggag cgtgatgatt gcctcgctgc
    13561 cgatgcgctc gatcaggccc ttggccagct gctgatccgt gtccttcgcg atctggtcct
    13621 cgtcggtcgc gatgatctgg aacttcagcg atgaactgcc gcaattgagg acgaagacgt
    13681 tcatgttggg aatcctgaca gggggcgatg agttccccga attatagtcg gaaaggcggg
    13741 gtgaccgcga aaccgggtcg cccgatgggg gtaaagtagc cgaggtcccc gaggggaggt
    13801 gcgcgtgaac ggcagaggat gggcgcgaag cctggtgctg gtggtggcgg cggtgggggt
    13861 gacggcggtt gcgcacgcgc agagcgcggg cgccagagcg gcggcgggcc cgttgcgggt
    13921 gacctcgccc ggcggcgcgc tcgaggtgac cgtcgcgacc gatggagttc tcacctggtc
    13981 ggcggcgcat cgcggccgga cgatcctccg cccgtcgcgt ctcgccctga cccttggaga
    14041 cggccgcgtg ctcggcaccg cgccgaaggt gacggcaacc tcagtgcggt ccatcgacca
    14101 ggcactgacg ccgcccgtcg cctaccggcg cgcgcgggtt gccgaccgct gccaggagcg
    14161 gcggatcgac ttcgcgggcg acttctcgct cgtcgtccgc gcctacgacg acggggtggc
    14221 gtaccggttc gtcacgcggc tgcccggcga aatcacggtg cgcgacgagg acgccacgct
    14281 ggtgttcgag ggcgaccacc tgctgtactt tcccgaggag gagagtttcc agtcccacca
    14341 ggagcgggag tacaagcggc tgcggattag cgagatcggc cccacccgtt tcagctccct
    14401 ccccgccctg gtcgtgatcg agggggggcc gaaggtggcg atcaccgagt ccgacctgtt
    14461 cgactacccc gggatggacc tcaagagcgg gtccgaagcg cacaccctgc ggggcctctt
    14521 cccggcgtac ccgcggaagg tgcggatcga acgcgaccgg gacaaggtgg tggtcgagcg
    14581 cgaagactac atcgcgaaga cgcgcggcac ccgcgagttc ccctggcgcg tgctcgtggt
    14641 ggccgagcgc gacgagaccc tgctcgacag cgacattgtc ttccgtctcg cctccgagag
    14701 ccggattgcc gacacgtcgt ggatcaggcc gggcaaagtg gcgtgggact ggtggaacgc
    14761 gaacaacgtc tacggcgtgc cgttccgcgc cggcatcaac accgacacct acaaacacta
    14821 catcgacttc gccgccgagc acggcatccc gtacgtgatc ctcgacgaag gctggtacaa
    14881 gctgggcgac ctcatggccg ccgcgcccga gctcgacatg ccggccctgg ccgcccacgc
    14941 gaagcagcga aacgtcgggc tcgtcatgtg ggtcgtgtgg aagacgctgg accagcagct
    15001 gcagccggcc ctcgaccagt tcgcgcgctg gggcgtgaag gggatcaagg tcgacttcat
    15061 gcagcgcgag gaccagtgga tggtgaactt ctacgagcgg gtcgcgcgag aggccgccgc
    15121 ccggcacctg ctcgtcgact tccacggcgc ctacaagccc accggcctgt accgcacctg
    15181 gccgaacgtc gtgaccagcg agggggtgct gggcctcgag cagagcaagt ggggcccggc
    15241 cgcgtcgccc gacaacgccg tcaccttccc gttcatgcgg atgctggccg gcccggtgga
    15301 ctacaccccg ggcgcgatgc tgaacgcgac gaaggccgac ttccgcccgg tcttcaaccg
    15361 tccgatgagc caggggacgc gcgcccacca gctcgcgatg tacgtcgtct tcgagagccc
    15421 gctccagatg ctggccgatt caccctcgaa ctaccggaag gagcccgaca gcctggcctt
    15481 cctctccgcc gttccggtgg tgtgggacga aacgcgtatc ttgagcgcga aggtggggga
    15541 gcacatcctg gtcgcgcgcc ggtcgggcag ggagtggtac gtcggcgccc tcacgagctg
    15601 ggagccgcgg gacatcgagg tggacctggg cttcctcggc gccgggtcgt tcaccgccga
    15661 catccaccgc gacgggccca acgccgaccg ggctggcgtg gactacgtcc gggagcagcg
    15721 gccggtcacc tcggctgacc ggctgcgcgt gcacctggcc cccggcggcg gcctggcggt
    15781 ccggatcgtg ggggccgtac cgaaagcgtg aaagaagtca cccgaggggc cgagtcacca
    15841 ggggggccgt accggaagtg tgaagaacgc atcgttcttg tacggctccc atggcagatc
    15901 tcggtcgttc agacgggcta tactcttctc tgggcttcct gcttcagtag cacaggcgtc
    15961 ccgcctgtgc cagtgcagcg cgacggcgcg gtctgcgcgc accttcagcc gaaggagaag
    16021 gcgatggctt cgttcgagcg acacagtgac gtcacctggg agggatcgat ccgcgaaggc
    16081 aagggggagg cggtggcggg gaccggcgcg ttcacgctgc cggtgacctt cccgtcgcgc
    16141 atcgaggacc cggctggccg gaccagcccc gaggagttga tcgcggccgc gcacgcggcc
    16201 tgctacgcga tggcgctgac ggccgccctg ggccggcgca acgcgagcgc cagccggctg
    16261 aaggtgacct cgacggtgcg cgccgagctg gcccagggca tcaccatcac cgcgtcgcac
    16321 ctgaaggtgg tggccgaggg gctgcagggg attagcgccg ccgacttccc gcagttcgcg
    16381 cgcgaggccg agcagggatg ccccgtttcg aacgccctca ggggcagcct gaagatcgac
    16441 gtggacgcgc aggcggtcta gcgccatggc ttcgctgctc gatcgcgcgg ggcgtccagt
    16501 tcccgacgcc gcgctcgccg agtttgcgag ggggttgcgc ggagcgaccc tctcgccggg
    16561 cgaccccggc tacgacgagg cccgccgcgt cttcaacgcg atggtcgatc gccggcccgg
    16621 cctcgtcgtt cagccgctgg atgccgccgg catccagcac gccgtccgtt tcgcacgccg
    16681 gcacgacgtg ctcgtctcca tcaagggagg cggccactcg gcacccggtc acgccgtctg
    16741 caacgacggc ctgttgatcg acctctgccg gatgcgtgca gttcgcgtcg atcccgaagc
    16801 tcgaaccgcg gtcgcagagg gcggtgccac ctggggcgag ttcgacgccg cgacgcaggg
    16861 gcacgggctt gccgtcaccg gcggccgcat ccgggggacc ggcatcggcg gcctcaccct
    16921 cggcagcggc tcggggtggc tcgaacgcaa gttcggctac acggtggaca gcctggttgc
    16981 cgccgacgtc gtgctcgcca gcggcgacct cgtcagggcc agcgaccacg agcaccagga
    17041 tctcttctgg gggcttcgcg gcggcggcgg caattacggc atcgtcgtcc gcttcgagta
    17101 ccggcttcac gcgctgggtc ccctcgtctg gggcgggatg ctgttgttcc cgcgcgacgg
    17161 ccgcgagcgc gaggtgctgc gggcgtatcg cgacttcatg gaagccgcgc ccgacgaggt
    17221 ggggggggct gcggcgctcg tcaccgtgcc gcccctgccg atgattcccg aggcggcacg
    17281 ggggaaggcg gcgctggcga tcatcgcggt ctatctcggg catcccggcg aggccgaggc
    17341 ggcgttcgag ccgatgcttc gactgcagcc ggcggcggcg ctcgtccagc cgatgccgta
    17401 cctcgccgtc cagggcctcc tcgatgaggc aaaccagcca gggaagcgca actactggaa
    17461 ggccgacacg tgtccgtcgc tgcccgacgc cgcgctcgac gcgttgctcg gcgcgacggc
    17521 acggccagcc tcgcccctga cgacggtcct tctccagccg atgggcggcg cggccgcccg
    17581 cgtccccgac gatgccaccg caatgggctg gcggcaggcg aagtggtcgc tgcacgtctt
    17641 ggggatgtgg gaggacgcct cggacgatgc acggcaggtg agctgggtgc gggcggtgac
    17701 cggcgccatg gcgccgtggg cccagaaggc cacctacctc aactatctga tggacgaggg
    17761 cgagggacgt gtcagagaca gcttcgggcc gcactacgcg cagatggtgg cgctgaagga
    17821 caagtacgat ccgaccaact tcttctgcat gaaccagaac atcaagccga ccgcccacct
    17881 gggaggcgtg gcggggatgt agaacgtgga gccgccgcag gcgcggcggc tctctcctcc
    17941 ggcggaggcg gcgactactt gccgagctgc aggatctgca gctgctgggc agccagggcg
    18001 tcggcgtgct tcagcaggac ctccgccgcc tggtcgccgt acttctggcg gacctcggcg
    18061 aggtcgcggc cgtcggtgaa ggtcttcatc accttctcgg cttcggcggt cgccgccgcc
    18121 cgctggtcct ccggcagcgc agcgatgctc gccttctgcg cggccgccat ctgctccagt
    18181 tgctggttgc cccccatctg gttgaacagg aggcggaggt tggcggtgag ctgggccgcc
    18241 tcgcgcaccg cgtcgaactg ctcgggcgtc aggccgaggg ccttgcgctt ggccgccagc
    18301 ttgtcgtcga gctccttgct gtactcctgg gcaatgccga cctgctggag ggccttcgac
    18361 gtgtcgccct tggcgctctc gaggttcttg cggctctttt cggcgtactc cttcacgagc
    18421 cgataggctt ccttctggta ttccatgtag ccggtcacca gcgcctcggt gatttcgacc
    18481 cgggcggccg gcgccgcagc ggtctcgggc gaagcggcta ccgccggctc ggcggcggcc
    18541 gacgtctcag gttccggctg ggcgggctct tcagatttgc cgccgcacgc ggcgagcaag
    18601 gccgtggcgg cgagcagggc ggtggcagca aacgggctga tgcgagtcat gtcgtctccg
    18661 tggcgggggc gacatactat accccgtagt gctgtttcag gaagctactg gggcggttcg
    18721 agcagcccgc gcagccgggc ggcgaacgcg gggcccaggc cctgctcctc gtgcttgaag
    18781 tacacgaaca cgtcgtcgca cccgcccgcc gcatcccgga tcgcccccgc ccaccggacg
    18841 atgtcggcgt cctggtagcc ctcgtcacgc agccggaagt aggcgtgggg cgcggtcatc
    18901 tccaccgggg tggaaagcct ctcgctgtcg gcaatgcaca gcgccgcgcc gtgcgaccgc
    18961 aggcagtcga acgactcgac gtcgtgccac gacgggtgcc tgaactcgaa ggccgggcgc
    19021 atcgccggcg agagcaggcg gaggaagtcg tgcagcaccc gcacgtcctt cttgaaagcc
    19081 gggggcagct ggaacaagag cgccccgagc ttcggcccga gcgccgcggc cgcacgccag
    19141 aacgtttcca cgtcctgggc gcagccacgc agccgggcca cgtgggtaat ccgttgcggg
    19201 gccttgagtg tgaaccggaa gccgtccggc gtggcgccgg cccagcccga caacagcgac
    19261 gccgtcggca tccggtagaa cgtgtagttg atctcgacgc tgcggaaccg ctccgcgtag
    19321 aaggggagca tcttcgcgct cgggaagcgc tcggggtaga aggtgccgcg ccattccggg
    19381 tagttgtacc cggacgtacc gatccagtac atggacgccg cctgttctga agccttttgc
    19441 cgctgcgatt atagcgtggt gtgaccagcc gtgcggcttc gtgccataat cggcgctgcg
    19501 gatttccgga ggattgccga gtgagaccgc tgcgtctgtt cgccctggcc gctcttgtcg
    19561 ccgcgctggc cgcgtgcggc gattcgccga ccgcgccctc gagcggcgtc ttcacccaga
    19621 ccgacctggt cgtcgggacc ggtgccgacg cgacggccgg caggaacctg acggtgcact
    19681 acacagggtg gctctgggac ccgtcgaagg ccgacgcgaa ggggctccag ttcgagacct
    19741 cccgcaccgg ccagcccctc gtgttcacgc tcggggcggg gcagctgatc gctggcttcg
    19801 accagggcct ggtggggatg aaggtcggcg ggctgcggcg cctgatcatc ccgccgtcga
    19861 tggcgtacag ctcgaaccgc aacggcccga tcccgcccaa cgcgacgctc gttttcgaga
    19921 tcgagctgct ggaggtcgag tagcgggggt tacgaagccc cgcccctgcc tcagccgccc
    19981 agcctgacgc gcagaccgaa gcggatcgtc gagatccgct cgtccagttc cccctcgccg
    20041 tcgtcccgcg cgccgccctt cagcgaaccg tagttcgcct cgccgtagag ggcaacccgc
    20101 cggctcagcc atccctcgag accgccgccg aagagccagc cccaccccgc ggtccgcaac
    20161 tccagttcct gcgtcccgcc cgggatcgtc tgctggacgc cgtcgatcgt gagcgtcctc
    20221 gtttcgatgg tttgggtggt gtggaaggtg gcgcggtggt gcgtggcgcc gccgcgcgcg
    20281 tagaagcgca ccgggccgac cggcacgccg acgagcagtg ccgccgtgat gacctgggcg
    20341 tcgagtgtcg attcgtagct gtaggtgctt tcctcgccgg tggccttcgc cgacgaaggc
    20401 ttcaggaagc cgccctcgat cccgaaatac tgcgacagcc agatcgacgc ccccaccgag
    20461 tagcccggct tgaacgcgct ggaatcgcaa tcgagcgtgc cgcaggccga ctgggtgaac
    20521 ctgctgaagt tggtgaagga gccgtcgccg aagagcatga ggcccttcgg ggccggccgg
    20581 cgcggccgct cggctggctc ggcctcgatg ttcaggagcc agtcgggcgg gacgggcccc
    20641 tgccgcaccc agagggtcgg cgccgccggc gcaagatcga cgagcaacga ggtcgaggac
    20701 tgaagcagga cgaactgtcc gacctcggtt cgggtgcacc ccgtctcggg cggcggcggc
    20761 tgcatcgtgc gctcgaccag cgtgatcgag cgaacggcct cgcagacgtc cacgagcacc
    20821 cgcgcgtcgg tctcggggcg gccaagcttc tcggggggga acgccaggcg ggcaatccca
    20881 tcggctcccg cgctggcccg cccgagcggt tcgccgttcc accgaagctc gatcgcggcg
    20941 ccggcgggcg cgttcttcac cacgaccgtc tgggcgccgg cgacgccgat cgccagggtc
    21001 acgttgagcg cggccgcgag cgccgcgcgc cgccagaatc gactcatgct cgttacctcg
    21061 cagggcccga cgtccggaga catggttgag tatgttaccg cacaagcccg ccgtcccggg
    21121 cgagggcagt tctgccatcc gcttcgacgc gggaaagcgg cttgcatgca ggagcctttc
    21181 gggtcggaat ctcccatgga cagggtcggc cgttaccaac ttctcgacaa gctcggcgaa
    21241 ggcggcatgg gcgtcgtcta caaggcctac gacccgctca tccagcgtgt cgtcgccgtc
    21301 aagctgatca gctcggcgag gaccgacgac ccggtgctgc gcgagcgctt cttcgccgag
    21361 gcccgggcag ccggacacct gtcgcaccgc aacatcgtga cggtctacga cctgggcgag
    21421 gagaacgagc gcccgtactt cgcgatggag ttcctcgagg gagaggatct cggccggatg
    21481 attcgccgcg gggcgcctcc caccttgatt gacaagatcg acctgatggc gcaggcgggc
    21541 cgcgggctcg cctacgcgca cgcgcgcggc gtgatccacc gcgacgtcaa gccggcgaac
    21601 atcttcgtca cgaccacagg cgacgtgaag gtgctcgact tcggtctcgc gcggctggcg
    21661 cgccccgtcg gcaccgactt gaccaggacg aggccgttcg tcggcaccgt cagctacatg
    21721 gcgcccgagc aggtccgcgg cgagccggcc gacgcccgga ccgacctgtt cgcattcggc
    21781 gtcgtcctct acgagatgct gagcggcggg cggcccgcat tcgagacaga ctcgttcgcc
    21841 tcgacgatgc atcgaatcct gactgaggag ccggagccgc tcgccgggct cgacgcgacc
    21901 ctgcccgagg agttgacccg cgtcgtcgag cgggcgctcg ccaagtcgcg cgacgagcga
    21961 taccagcggg tggacgagat gctggccgat ctcgctgcct tccgggcgag cctgaacgcg
    22021 ggcgccgagc cgcacgcccg gttcaccccg acgcccggtc cgcgccgcgc cagcctcgag
    22081 gggctcgacg tcaccgtcgg acacgaggcg gccgtggacg tgcgcacgcc gcctccccgg
    22141 gcccttcccg cgtcgccggt tcccgcatcc ccgtccccga cccccgtgag ctccgtgcca
    22201 g
//
LOCUS       DAOPQX010000017        21393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3053_length_21393_cov_4.785172, whole genome shotgun sequence.
ACCESSION   DAOPQX010000017 DAOPQX010000000
VERSION     DAOPQX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 21393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21393
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3053_length_21393_cov_4.785172"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="PKK95_01890"
     CDS             <1..1402
                     /locus_tag="PKK95_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC16984.1"
                     /translation="APADLPRLNEVSTNGVVLAFAVGLSALVAVGLGLVTAWRATRRD
                     PREALSDARGQAGGGSSRVGRVIVAAQVAITVVLLVGAALLGRSLLRVLAVDPGFRTE
                     GLVAMDLSFPYSEDPAARARLTPFYTATFERLRAIPGVEDVAAASRVPMDGGLPDGLF
                     AVLRPGEEAPKTFDDLHGLFEQKDRLGTADFCAASPAYFRALGIPLVKGRLLDERDGP
                     DAPHATVINESLARAQWPGADPIGRTIEFGNMDGDLRPLTVVGVVGDTREYGPERPPR
                     PTLYVNLLQRPRFWVTVVIKASGDRAPVIAAARGVLRELAPEVPPRFRTFEQIYSAAL
                     GGRYFNLALLGAFAGTALILAVAGIYGVMAYSVTRRRREIGVRVALGASPGHVRRLVL
                     GQGLATTAAGVVCGLVGAFALTRTLESLLFGVTPTDPATFVLVIAVLAGAALVACYVP
                     ARRATLSDPVVAMREE"
     gene            complement(1562..2809)
                     /locus_tag="PKK95_01895"
     CDS             complement(1562..2809)
                     /locus_tag="PKK95_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOC16985.1"
                     /translation="MIDKVLGFTLVLVLVLGGAGSVSAQPSGFLRANGDRIVDGRGRE
                     VLLRGMGLGGWMLQEGYMLGIKKEGTQHTIRARITDLVGEKDAERFYRLWRKNHMTRA
                     DVDLLARSGFNSIRLPMHYNLFTLPIEAERVKGHDTWLKTGFDMVDDLLAWCRANRMY
                     LILDLHAAPGGQGKDANISDYDPSKPSLWESEEDQRKTVALWRKLAERYAGEPWIGGY
                     DILNEPNWTFEGKDKNGREDVSNAPIWDLYKRITQAIREVDRRHLIIVEGNGWGNNYN
                     GFPDPWDGNLVMSFHKYWNPNTEEAIAPFLALREKYRLPIWLGESGENTNEWFSECVA
                     LVEKHGIGWAWWPYKKIGNPRCILTVTAPDDFAKVVAYWNGEGERPSREAARRGLFQL
                     VENLKRSKCRFNAEVAQALIPNP"
     gene            complement(2924..3481)
                     /locus_tag="PKK95_01900"
     CDS             complement(2924..3481)
                     /locus_tag="PKK95_01900"
                     /inference="COORDINATES: protein motif:HMM:NF037864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOC16986.1"
                     /translation="MCSRAKLAYESTAAWLEGEGPMPPAVARVPGLASLLRERAGEDG
                     PGHFGLAATDYTHSTAPNRRFPDLVTQRLVKAALAGAAVPYSEDQLEQLAAHCTDMED
                     EARKVERRVKKAAAALVLESRVGQVFDAVVTGAAKKGTWVRLLAPPVEGRLERGFEGV
                     DVGDRVRVRLIGTDVERGFIDFAIA"
     gene            complement(3616..4119)
                     /locus_tag="PKK95_01905"
     CDS             complement(3616..4119)
                     /locus_tag="PKK95_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOC16987.1"
                     /translation="MSDYPGRLGEAIETLSFATDPADRADLLLSFANRFREVPPEVAR
                     RPFPEINRVPACESEAFAWGVVQDDGMLALHFAVENPSGVSAKALAVILDRGLSGLPP
                     SEIARVSPDIVYDIFRRDISMGKGMGLMSMVRAVQVIARQAAEAAADGSEAARIFPRR
                     PVERPCR"
     gene            complement(4226..5107)
                     /locus_tag="PKK95_01910"
     CDS             complement(4226..5107)
                     /locus_tag="PKK95_01910"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOC16988.1"
                     /translation="MEAVLKEIDVRGYAHPEALVTTEWVQQNLNNTAIRLIESNEDTL
                     LYASGHVPGAVHVDWTADLNDQVRRDYIGSDAFGALMDRLGVTPETTVVFYGDKNNWW
                     ACYAMWVFELFGHTRARIMDGGRLKWQKEGRPLTREIPRFSGAGYSAPGRDDRTNRAF
                     RDEVLAFANADGQLVDVRSPEEYWGKRLHMPDYPNEGALRGGHIPGAKSIPWARAVNP
                     EDGSFKSGPELRRIYVEEQGLTPGREVIAYCRIGERSAHTWFVLKYLLGFDRVRNYDG
                     SWTEWGNLVGVPIEREQ"
     gene            complement(5219..6403)
                     /locus_tag="PKK95_01915"
     CDS             complement(5219..6403)
                     /locus_tag="PKK95_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOC16989.1"
                     /translation="MNSFTYYLPTRLIFGPGKLAELATTPHFPWKKALVVVGQGGSTR
                     RSGSLERVRSLLASRGVEVVVYDRIRPNPEAAQVEEAAAIARAEGCDVVVGLGGGSSI
                     DSAKSIAVMAKNPGRYWDYMMSAAGGRKKPEQGALPIVAITTTAGTGTEADPWTVVSN
                     PDAREKVGWGCDFTFPALSIVDPELMVTLPPKQTAYTGMDALFHAVEAYLATCHQPAS
                     DLFALEAVSLIARWLPVAVADGTNMEARTALAWANTAAGICETLSSCISHHSLEHAVS
                     AFYPHVPHGAGLTMLSVAYFTDVAERNPTRFADLARAMGEDVDSLSEGDRPFAFVTAL
                     EKLIAASGLAGETLSGYGVNESDIPALAANSLSAMGGIFACTPVKQDQADVERIFRRA
                     FK"
     gene            6619..7827
                     /locus_tag="PKK95_01920"
     CDS             6619..7827
                     /locus_tag="PKK95_01920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC16990.1"
                     /translation="MAIISRRTCLSGLGVAAAALGAARAGRVRAAGVPAPEGALQAAS
                     QAPARLDITEFQPRSMLHVPATRVERARFPVIDVHTHLGFSGREGMSFAARPETLLPV
                     MDRKNVRMMVNATGGSGRGLDESLERFDRRVPGRFVTFAEPRWEGFNQPGWGQRQAEE
                     LGRAREAGARGLKVLKTLGLGLRENGTTGALIKVDDPRLDPMWDACAALRMPVLIHVA
                     DPEAFFLPIDRFNERFEELNNHPDWSFHGADFPSFRELVDAFDRIVARHPKTQFIAAH
                     FGLNAENLPSVSERMDRYANLHVDTAARIGELGRQPRFARRVFERHQDRILFGTDAVP
                     DGHDTPQQVFGDDLYEIYFRCLETEDEYFDYAPAPVPPQGRWAIYGLGLPEGILRKVY
                     HDNAARLLGL"
     gene            complement(7880..13459)
                     /locus_tag="PKK95_01925"
     CDS             complement(7880..13459)
                     /locus_tag="PKK95_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOC16991.1"
                     /translation="MEQGASIARSARTDRWRVVGPGGGGTTLHPVISPHDSNVAAQTC
                     DMTGSYLTVDGGVSWRMFNLGTGASAFAFDPNDPSVIYAAASAIFRSDDRGKTWAMVF
                     PDPARNTVANGWGDHAETVYTTDDPLYPSGQDVVIHAIGVDESDSNRLFAAIQARKPG
                     PPGSSEPETTTVLASANRGRTWTAIGDLPAQRVFAIWAGIAGGPVQLLAEEGVFVRAG
                     DRWRRGPAPAGVRFDSGSVGRDAEVGATLVYATAPLEAGEAGVRGGMYVSRDSGAHWV
                     EANGRLSELFGDAVGRGETWGPAHTSRPRLGPVSASARHGLTAYVGLRGLRAKDGDGK
                     PFNGVARTADGGRTWTIVHRESDRPAANFEPSWIEARAVEDGRSVWLDAPYDIGAAPS
                     DPLVCHVTDLFRTYRTTDGGRTWAQVNSARANGDAWVSRGLDVTTHYGMAWDPFDPKH
                     VLVATTDIGLFGSEDGGATWIGSSSGIPRPWRNTAYWVVFDPEVKGLVWGGFSGTHDL
                     PRPKMWRRTDPASFRGGVAVSTDGGRNWTPSNGAMAESAVTHLILDPRSPKGQRTLYA
                     AAFGRGVYKSIDNGRTWTLKNAGLAPDPREQPFAWRLAQDPAGLLYLVVARRSERGRI
                     GNADDGALYVSSDGADHWRKLDLPPGVNGPNGLAIDPREPRRLYLSAWAVATPGGDTG
                     GGIFISDDGGGTWRRARGATGHVYDVTIDPRNPDVMYACGFDQAVLRSVDRGETWHRV
                     PGFNFKWGHRVVIDPADPSSIYVTTFGGGVWHGPAAGDATSTEDVTTGRERLARLVEA
                     NIAGVHAFQVLLARRSGKGDAACYAGEGLTDAQLEALVEHQAALLASDRDQVAAWAAG
                     QASSFDPAKDLAPLESSGLKLSERLPVNVFAADLSRQKAAPPLSLRAIANLYQTVLEV
                     ERDGDLLQDLYRFYIALGLPVYLGPLGLSGSDAAMLEAGRRLEGRACASPFGLTAAEW
                     QLAGRKIWNWGEKNLRVRDAHVLAGELLEEPDVARLIPGMRAMPAQRVAVIGHSFTMD
                     LHWSSPSAFAPIVEAMFQRENPAVAFRFFQGGGLTSSRALKRFYQDVLAWKPQTVLLV
                     VANRTEEDFEALKTMVGGLKAAGARVFVFDDVLDPLDREPEVMARQAAVARSSGAIVV
                     EVRRLLEASPDRGRFLCLDGIHMTEPYHRLMAKQWLALLAGANGGAGRASEVPPVEAF
                     TVLDPQPPAGPRITPYLRYQLDRAWAQDSKRLADWAAVRNEADLEALKRRTRERLLAA
                     IGGLPNERTPLNARIVGTVPAEGYRIEKVIFESVPGLHVPALLYLPDGPARPRPAVLL
                     PCGHSPLGKAFRNYQEIAVRLVRRGYVVLCWDPVGQGERSQFWDEARGRSRYNLVCGE
                     HAVLGNLACLAGANLARWEAWDGIRALDYLLTRPEVDRRRVAITGTSGGGFQSATIGA
                     LDERIAVVAPSCYITSLPMRMANRIFEDPDSDPEQDPYGSVSGGIDHPGLLLLVHPRP
                     LIVASAVKDFVPIEGARRTFREIEAIYRRFGGAGRVAMVEGYHEHRFSDENQDAVFAF
                     LDRFNGLSARHGFEPFTPLPAEALRCTATGQVRVDLEDDRPLVEIIRDYHRATRGSGA
                     GTLGALYRSASFPRIDAWPVVPWDRVTPRAAIAWEARGTTAVGGISIDRYVVRHSGGL
                     VMPLLHVHPTGVEHPTATPARPVLLDLSLDGKATPADWAALRARLDAGFDVVSFDLRG
                     TGETRMRYRAASVDDPTLAPADEEAAYADPLSGVLANYVYNSLLTGRPYFLEMLEDVE
                     VAARFSRAKLGAARVAVAGRGDASLLARAAGDVLPGVEWIPGDPADPAFSWSEAVESL
                     TETWPIQYLLPGGARLR"
     gene            complement(13552..14547)
                     /locus_tag="PKK95_01930"
     CDS             complement(13552..14547)
                     /locus_tag="PKK95_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC16992.1"
                     /translation="MRFCLIGCGEHAVSSHGPSLALYAASHPGLELAGCCDTDAAKAE
                     AFRERFHFARAYADLDSMLDAERPDAVAVVVPVRFACHVGTRVLSRGIPLLLEKPPGE
                     RVGEVDRLAAAARERGRDGRAIPHQVAFNRRYAPLVVRLRERVEQLPDSAPLQHIRYE
                     MVRVNRRDPDFSTTAIHAIDAVRFIAGSDYGEIRFRYQPLPGLGPGVANIFMDAVLRS
                     GVTAHIACCPVAGVVVERAELHARDHTLFVEIPMWGGYDTPGRLVHLERATVVEDVRG
                     AAGAGPVVLGGFYGEYEAFFASLAAGLPPRPSLEAVRQSVEVAEAIRARREEWRS"
     gene            complement(14555..15598)
                     /locus_tag="PKK95_01935"
     CDS             complement(14555..15598)
                     /locus_tag="PKK95_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16993.1"
                     /translation="MFSTTRSHLVVLCLSAAAAAAGACANAPAQQSPPPMPAITEPVM
                     FDTPEADRILTALQVFPPDNPWNQDISALPVHPDSQKMIAIIGPDKPLQHNLDMGFII
                     VPPNQPRVPVKLLDYPEESDPGPYPVPDNAPIENWPMQRNEDVDALPKPGQTLEQLQR
                     EGTGDRHIIVVDPGNGKLYEFWQARKTDAGWEASNNATFDLKTGALRPERWTSADAAG
                     LPIFPAVVRYDECERGMVEHAMRFTVRRTRRAYVLPATHWASRETDPALPRMGERFRL
                     RKDFDVTPFPPHVQAILKGLKKYGMFVADNGSDWHMSIAPDRRLKGLETLSQIKGSDF
                     EVVDTGARSVEGQ"
     gene            complement(15620..17938)
                     /locus_tag="PKK95_01940"
     CDS             complement(15620..17938)
                     /locus_tag="PKK95_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOC16994.1"
                     /translation="MRRCSSLLVLGVCACWLAVAGANDRPRMLLAASDPFTSLAALEA
                     KYAAGERPSDDLPGWALTWLLTKDESFARRAVDEMRRVRLPVPRNSRPYFQYVARALA
                     FDWLYGSPLFDAGLKDQVAQELVAGAEQMLALQSLADPASASFHNHSIRELTLATFAL
                     AAAERHPPTAARASLLLAHAGRALDNMLETSELVAPSGGYHESMDYMRITFTPLAMLA
                     ELRRTTTGEDPARRWGVFANMGPGYLYKVLPDGSAARDDDNEFPPLDAVDTVVLGYAV
                     HRFKDPYAAWFLQQSGWLPKEWRLPVLEFLWSDRSVAPRDPRLARPEELPRQRLFPGI
                     NHLVMRGGWEPGDTWIEFSCGPYFAKHDHLDTNHFAIFRKGHLALDSGADYTDTESPH
                     YLNYYRRTVAHNTILVYQPGETFFWGENLWPAANDGGQRMDSSRFWNSVRSLEDWERT
                     RDLWDRGRIDAYEARPGAYTYVRGDGTAAYQPSKLRRFVRDLLWLEAADVLFVSDDVR
                     SVDPSYRKAWLLHGVNAPAVEGADGPQRDVGHGGTSWPNASLATFEEGGGRLRVHAVL
                     PQSREVIVRGGPGWEFWTPGDEKGGEWGTGANWPLDPPEGGALPADPVLRRMWKTFWG
                     PDFERLSPSNRRSVVPGAWRLEVTPAVPARDDRFLHVLEIGDRGAPPRRIEAVRGHNL
                     AGAVVEGHGGALFVTSPGFPAAAEVTLPDLAAPALVLAGLEPHRSYEVQLTSNFAPGS
                     PAGRLLTQASAAGTARVDLGERRGVRLRLRPM"
     gene            complement(17949..19436)
                     /locus_tag="PKK95_01945"
     CDS             complement(17949..19436)
                     /locus_tag="PKK95_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOC16995.1"
                     /translation="MRTLKTILLAAVASLLAAPAAAQRLEPPVREWFDQHRLLLVPEE
                     VEVLRRVKADDLETFVRIFWARRDPASGSPENRFRAAADRARADADARFGEAGKKGSV
                     TACGQVVMLLGHPDEVTGRELRNIFDTRPSAPSRYRTREPVSRNATRDGARSPELWVY
                     KSSPARTFRMPGGDLKLQFDDGCEFEESARTLDELARIAAGLILHPEIGYEFGADGRL
                     RPLAAPPASGAAALLDRPSADFTLSFELKIQVPAQAGSYVAGLLRGAPGSLPVPESEK
                     FVRLKAIARAAGSSGSAVPGDEREVVAEVAPDGSFVSSHGLSLPPGRFAVTLAVLDPA
                     TGRGATTTLDVESPDYAGSSLVVSPLAVLAGLEEATAAPAGPDPYAAFAIGSQRLRPR
                     AGNVLDQAGSLRLLVLVHNARLDPATGRASIKASFSVLRDGAAVAKSSEQAFDTPGAA
                     PSVGPISLAPFSPGRYVARVEIADEVGHTAVVRETPFEVTPAAGR"
     gene            complement(19520..>21393)
                     /locus_tag="PKK95_01950"
     CDS             complement(19520..>21393)
                     /locus_tag="PKK95_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16996.1"
                     /translation="EASLQKIRFTGNNNRTIEQNSFSIADNISWMAGGHSLRGGVLIG
                     RNSAIDGFGRGLNHRGAYRFNGGFTGNAFADFLLGMPTRVDEHVSTRGDLNGYSWDFA
                     TFVQDDWRVNKDLTLFLGLRYELAGGWNEKNNMLANFQAKDGGYHVVADQTVWSLLPY
                     GLSNPDSPYYSHVKLAGDLSLPKTLIKTDTNNFSPRVGFAYRLDGQGRTVLRGGFGLF
                     HPTTAIQGIRDQLATNEFRYTIQRQPGPFANAFSQGVGMPGNDDFGTQGVWPDVQSPD
                     IYQYNVTLERELPWNMGVRVSYLGSTMRKLLVNREYNSVKPSTEDLYNWDPSYNYRRP
                     FPMYGPYVNMIENTGSGRFNAAQLELTRRFRNGFALDLAYTYATSSSNAPDSGNSSLG
                     VLQYDPYNLEADRGPDPYVVKHRLILNATVDVPVGRGRAYAADMPAWADALFGGWTVS
                     AIFQARSGANLTPYFVLSSSFTPYNIGFNPDTTGVWVGDSYRGDQIGDPKANVPSGMF
                     FNPGAYEMPPPGKFPGNTKRNSLEGPGNWIVNLAFYKDVVSIRGAKLQFTCLLDNAFN
                     RKQFFPGPGSEFLDMTDYLVNGIEDNGTTGVLGSTAEANVEGFALSRQIRLGLRMRF"
BASE COUNT         3071 a         7510 c         7633 g         3179 t
ORIGIN      
        1 cgcgcctgcc gatctgcccc ggctgaacga ggtctcgacg aacggcgtgg tgctggcctt
       61 cgccgtcggc ctctcggcgc tggtcgccgt gggcctgggc ctggtgactg cgtggcgggc
      121 cacgcggcgc gacccgcgcg aggcgctctc cgacgcgcgt ggccaggcag gcggcggcag
      181 ctcgcgcgtc ggacgcgtca tcgtcgcggc ccaggtcgcc atcaccgtcg tgctgctggt
      241 cggcgcggct ttgctcggcc ggagcctgct gcgcgtgctg gcggtggacc cggggttccg
      301 gaccgagggc ctggtggcga tggacctctc gttcccctac tccgaggacc cggcggcgcg
      361 agcacggctc accccgttct acacggccac cttcgaacgg ctgcgcgcca tcccaggggt
      421 cgaagacgtt gccgcggcca gcagggtgcc gatggatggc ggtttgccgg acggcctttt
      481 cgccgtgctg cggccgggcg aggaggctcc gaagaccttc gacgatctcc acggcctctt
      541 cgagcagaag gacaggctcg gcacggccga cttctgcgcc gcgtcgccgg cgtacttccg
      601 tgcgctgggg attccgctcg tcaagggccg gctgctggac gagcgcgacg gccccgacgc
      661 cccgcacgcg acggtcatca acgagtcgct ggcgcgagcg cagtggcccg gcgccgaccc
      721 gatcggccgc acgatcgagt tcggcaacat ggacggcgac ctccggccgc tcaccgtcgt
      781 gggggtcgtc ggcgacacgc gcgagtacgg gcccgagcga ccgccgcggc ccaccttgta
      841 cgtcaacctg ctccagcggc cccgcttctg ggtgacggtc gtcatcaagg cgagcgggga
      901 tcgcgccccg gtgatcgcgg ccgcgcgcgg ggtgctgcgc gagctggcgc ccgaggtccc
      961 gccccgcttc cgcaccttcg agcagatcta ctcggcggcg ctcggcggcc ggtacttcaa
     1021 cctggcgctg ctcggggcat tcgccggcac cgccctgatc ctcgcggtgg ccggcatcta
     1081 cggcgtgatg gcctacagcg tcacccggcg ccgccgcgag atcggcgtgc gcgtggcgct
     1141 cggcgcgagc cccggccacg tccgccggct cgtcctcggg cagggcctgg ccacgacggc
     1201 cgccggcgtg gtttgcgggc tggtgggggc gtttgcgctc acccgaacgc tcgagagcct
     1261 gctcttcggc gtcacgccaa ccgacccggc caccttcgtg ctggtgatcg cggtcctggc
     1321 gggcgccgcg ctcgtcgcct gctacgtccc cgcgcggcgg gccacgctgt ccgatccggt
     1381 ggtcgcgatg cgggaggagt gacgagcaga cggtaagcgc tgccaagcgt cagcacaggg
     1441 cgtgagcggg tgacgcgggg cggagggccc ccgctcgcct ggagcgcgac acgcgtgaca
     1501 agtgcttttc gcgcgaagcc gagcgggggg agccccgcgg caggacccgc gaacgccgcg
     1561 gtcaggggtt tgggatcaga gcctgggcga cctcggcgtt gaagcggcac ttcgatcgct
     1621 tcaggttctc gacgagctgg aagaggccac ggcgggcagc ctcgcgcgac gggcgctcgc
     1681 cctcgccgtt ccagtaggcc acgaccttcg cgaagtcgtc gggcgcggtg acggtgagga
     1741 tgcagcgcgg gttgccgatc ttcttgtacg gccaccacgc ccacccgatg ccgtgcttct
     1801 cgaccagcgc cacgcactcg ctgaaccact cgttcgtgtt ctcgccgctc tcgcccagcc
     1861 agatcggcag gcggtacttc tcccgcaggg cgaggaacgg cgcgatcgcc tcctccgtgt
     1921 tcgggttcca gtacttgtgg aagctcatga cgaggttgcc gtcccacggg tcgggaaacc
     1981 cgttgtagtt gttcccccag ccgttcccct cgacgatgat gaggtgccgg cggtccacct
     2041 cgcggatggc ctgcgtgatc ctcttgtaga gatcccagat gggcgcgttc gacacgtcct
     2101 cgcgcccgtt cttgtccttc ccctcaaagg tccagttcgg ctcgttcagg atgtcgtagc
     2161 ccccgatcca gggctcgccg gcgtagcgct cggcaagctt gcgccacagc gccaccgtct
     2221 tgcgctggtc ttcctcgctc tcccagagcg acggcttcga cgggtcgtag tcggagatgt
     2281 tcgcgtcctt cccctggccg ccgggggccg cgtgcaggtc gaggatcagg tacatccggt
     2341 tggcccggca ccaggcgagc aggtcgtcca ccatgtcgaa cccggtcttg agccaggtgt
     2401 cgtgtccctt caccctctcc gcctcgatcg ggagcgtgaa gaggttgtag tgcatcggca
     2461 ggcggatgct gttgaagccg gagcgagcca gcaggtccac gtcggcgcgg gtcatgtggt
     2521 tcttccgcca gagccggtag aaccgctcgg cgtccttctc gccgaccagg tcggtgatgc
     2581 gcgcgcggat cgtgtgctgc gtgccttcct tcttgatgcc cagcatgtag ccttcctgga
     2641 gcatccatcc cccgagcccc atgccgcgca gcagcacctc gcggccccgc ccgtccacga
     2701 tgcgatcgcc gtttgccctg aggaatccgg agggctgcgc ggacaccgat cctgccccgc
     2761 cgagcacgag caccagcacc agcgtgaacc ccagcacctt gtcaatcatc ggcctcgtct
     2821 cccgccctcc gcgcctccac ccggagcgcg gacatgaatg cagcgtgaca gtgtgacggc
     2881 gtagcctctg tgccgctgcg cctctgtggt gaacgccgac acgtcacgcg atcgcgaaat
     2941 cgatgaatcc ccgctccacg tcggtgccga tcagtcgcac gcggacccgg tcgccgacgt
     3001 ccaccccctc gaacccgcgc tcgagccgcc cctcgacggg cggggccagc aggcggaccc
     3061 acgtcccctt cttcgccgcg ccggtgacga ccgcgtcgaa gacctggccg acgcggctct
     3121 cgagcacgag ggcggcggcg gctttcttca cgcggcgctc caccttgcgg gcctcgtctt
     3181 ccatgtcggt gcagtgggcc gccagctgct ccagttggtc ctcggaatac gggacggcag
     3241 ctcccgcgag cgctgccttc accagccgct gggtgaccag gtcggggaag cgccggttcg
     3301 gagcggtcga gtgcgtgtag tcggtggcgg ccaggccgaa atgacccggg ccgtcttcgc
     3361 cggcacgctc gcgaagcagc gaggccaggc cgggcacgcg ggcaaccgcc ggcggcatgg
     3421 gcccctcccc ctccagccag gccgcggtgc tctcgtacgc gagcttggcg cggctgcaca
     3481 cgagcgccgg gtagacctcg gcctcggcga cagctgtcgg tatcgctgcc gcaggacgac
     3541 gggtcgatcc gggtcctggt tgccgtggcc gaccatgccc gcgattctat cggaagtcgg
     3601 ggtggcgggc gccgcttacc ggcagggacg ttcgaccggg cggcggggga agatgcgagc
     3661 ggcctcactg ccgtcagccg cggcctcggc ggcctgccgc gcgatcacct gcaccgcccg
     3721 caccatcgac atgaggccca tccccttccc catggagatg tcgcggcgga agatgtcgta
     3781 gacgatgtcg ggagagaccc tggcgatttc ggagggcggc agccccgaga gcccgcggtc
     3841 gaggatcacc gcgagcgcct tggccgagac gccgctcggg ttctcgaccg cgaagtgcag
     3901 cgcgagcatg ccgtcgtcct gcaccacgcc ccacgcgaag gcctccgact cgcacgccgg
     3961 cacgcggttg atctcgggaa agggccgccg ggccacctcc ggcggcacct cacggaaccg
     4021 gttggcaaag gagaggagca ggtcggcgcg gtcggccggg tcggtcgcga acgagagggt
     4081 ttcgattgcc tcgccgagcc gtcccgggta atcgctcacc aacccctccg gttccctgtt
     4141 ccccgttccc aattccccat catgacacca gcacgcccac ggcagtgccg gtccgcgctt
     4201 gccgtcttcc gccttccgtt ctccgttact gttccctttc gatcggcacg cccaccaggt
     4261 tgccccactc ggtccagctg ccgtcgtagt tgcggacgcg gtcgaagccg agcaggtact
     4321 tcagcacgaa ccaggtgtgc gcgctgcgct cgccgatccg gcagtacgcg atcacctcgc
     4381 ggccgggcgt caggccctgc tcctcgacgt agatccgccg aagctccggg ccgctcttga
     4441 acgaaccgtc ctccggattc acggcgcggg cccacgggat gctcttcgcg cccgggatgt
     4501 ggccgccgcg cagggccccc tcgttcgggt aatcgggcat gtgcagccgc ttgccccagt
     4561 actcctcggg gcttcgcacg tccacgagct ggccgtccgc gttggcgaag gcgagcacct
     4621 cgtcgcgaaa cgcacggttg gtgcggtcgt cgcggccggg ggcgctgtag ccggcaccgc
     4681 tgaagcgcgg aatctcgcgc gtcaggggcc ggccctcctt ctgccacttc agccggccgc
     4741 cgtccatgat gcgcgcgcgg gtgtggccga acagctcgaa cacccacatc gcgtagcagg
     4801 cccaccagtt gttcttgtcg ccgtagaaca ccacggtcgt ctcgggcgtg acaccgaggc
     4861 ggtccatcag ggcgccgaag gcgtcggagc cgatgtagtc gcggcgcacc tggtcgttga
     4921 ggtcggcggt ccaatccacg tggaccgccc cggggacgtg accggaggcg tagaggagcg
     4981 tgtcctcgtt cgattcgatg agccggatgg cggtattgtt caggttctgc tgaacccact
     5041 cggtcgtgac cagagcctcc ggatgggcat acccgcgtac gtcgatttcc ttgagcaccg
     5101 cttccatggg ggccatcctt tcaagcgggc ggggcgccgc ccggtgaagg tcaggggtat
     5161 cttcgaaagt atgcttcgat tatgcggccg gccggggtcg ccgctcaagg cgaccacctt
     5221 acttgaaagc tcggcggaag atgcgctcca cgtcggcctg gtcctgcttc acgggcgtgc
     5281 aggcgaagat tccgcccatc gccgagagcg agttggcggc cagcgcgggg atgtccgact
     5341 cgttgacgcc gtagcccgac agcgtctcgc cggcgaggcc ggaggcggcg atcagcttct
     5401 cgagcgcggt gacgaaggca aagggccgat cgccctcgga cagcgagtcc acgtcctctc
     5461 ccatcgcccg cgccaggtcg gcgaagcgtg tggggttgcg ctccgcgacg tcggtgaaat
     5521 aggcgaccga gagcatcgtg aggccggcgc cgtgcggcac gtgcgggtag aaggcgctca
     5581 cggcgtgctc gagcgagtgg tgcgagatgc acgacgagag cgtctcgcag atccccgccg
     5641 cggtgttcgc ccaggcgagc gcggttctcg cctccatgtt cgtcccgtcg gccaccgcca
     5701 ccggcagcca gcgcgcgatg agcgacaccg cctcgagggc gaacaggtcg ctcgccggct
     5761 ggtggcacgt ggcgaggtag gcttcgaccg cgtggaacag cgcgtccatg ccggtgtagg
     5821 cggtctgctt cggcggcagc gtcaccatca gctcgggatc gacgatcgac agcgccggga
     5881 acgtgaagtc gcagccccac ccgaccttct cgcgcgcgtc gggattgctc accacggtcc
     5941 aggggtcggc ctcggtaccc gttcccgccg tggtcgtaat cgccacgatc ggcagtgcgc
     6001 cctgctcggg cttcttccgc ccgccggcgg ccgacatcat gtagtcccag tagcgccccg
     6061 ggttcttcgc catcacggca atgctcttcg ccgagtcgat cgacgagccg ccgcccagcc
     6121 cgaccacgac gtcgcagccc tcggcccgcg cgatcgcggc cgcctcctcc acctgcgccg
     6181 cctcggggtt gggacggatc cggtcgtaga ccaccacctc gacgccgcgc gaggcaagca
     6241 gcgaccgcac gcgctcgagg ctgcccgagc ggcgggtcga ccctccctgc cccaccacga
     6301 cgagcgcctt cttccagggg aaatgcgggg tggtggccag ctcggccagc ttcccggggc
     6361 cgaagatgag gcgggtcggc aggtagtacg tgaacgaatt catcgaagct ccttcgtgtg
     6421 ggaatcgggc atcaacgtgc actcgtgagc gcgatcatag cgcgcctggg tcccgccgtc
     6481 gccgcgagcg tagcgagcgc ctggcaagag ggcttgtccg ccggagccgc gagcgtagcg
     6541 agcgttcggc gaaggcggat tgacacgagc actcgccgcg atatacagac acagggccct
     6601 ggaggaggtg aaggaccgat ggccatcatc agccgccgca cgtgcctgtc gggactcgga
     6661 gttgccgccg ccgcgctcgg cgccgcccgc gccgggcggg ttcgcgcggc cggcgtgccc
     6721 gcaccggaag gcgcccttca ggccgcctcc caggcgcccg cgcgcctcga catcaccgag
     6781 ttccagccgc gcagcatgct ccacgtgccg gccacgcgcg tcgaacgggc gcgcttcccg
     6841 gtgatcgacg tccacaccca cctcggcttc tcgggccgcg agggcatgag cttcgccgcc
     6901 cggcccgaaa cgctcctgcc ggtgatggac aggaagaacg tgcggatgat ggtgaacgcg
     6961 accgggggat cgggcagggg gctcgacgag agcctggagc ggttcgacag gcgggtgccg
     7021 gggcggttcg tgaccttcgc cgagccgcgg tgggagggct tcaaccagcc cggctggggg
     7081 cagcgccagg ccgaggaact ggggcgggcg cgcgaggcgg gggcgcgggg cctcaaggtg
     7141 ctgaagacgc tcgggctggg gcttcgcgag aacggcacga ccggcgccct gatcaaggtg
     7201 gacgacccgc gcctcgaccc gatgtgggac gcgtgcgccg cgctccggat gccggtgctg
     7261 atccacgtgg ccgacccgga ggcgttcttc ctcccgatcg accggttcaa cgaacggttc
     7321 gaggagctga acaaccaccc cgactggtcg ttccacggcg ccgacttccc gtcgttccgc
     7381 gagctggtgg acgccttcga ccgcatcgtc gcgcggcatc cgaagacgca gttcatcgcc
     7441 gcgcacttcg ggctgaacgc cgagaacctg ccgtcggtgt cggaacggat ggaccgctac
     7501 gccaacctgc acgtggacac ggcggcgcgg atcggcgagc tgggccgcca gccgcgtttc
     7561 gcgcgcaggg tgttcgagcg gcaccaggat cggatcctgt tcgggaccga cgcggtaccc
     7621 gacggccacg acacgccgca gcaggtgttc ggtgacgacc tctacgagat ctatttccgc
     7681 tgcctggaga ccgaagacga gtacttcgac tacgcgccgg cgccggtgcc gccgcagggc
     7741 cgctgggcga tctacgggct gggccttccg gaagggatcc tgcgcaaggt gtaccacgac
     7801 aacgccgcgc gcctgctcgg gctctaacga accggcgttc agcgccctcc gccttccgcc
     7861 cccgctccca tgcctttcgt cagcgcagcc gcgcgccgcc cggcagcagg tactggatcg
     7921 gccaggtttc ggtaagcgac tcgaccgcct cggaccagga gaacgcgggg tcggccgggt
     7981 cgccgggaat ccactcgacg cccggcagca cgtcgcccgc ggctcgcgcg aggaggctcg
     8041 cgtcgccgcg ccccgccacg gcgacccgcg cggccccgag ctttgcgcgc gagaagcgcg
     8101 cggcaacctc gacgtcctcg agcatctcga ggaagtacgg gcgcccggtg agcagcgagt
     8161 tgtagacgta gttggcgagg acgcccgaga gcgggtccgc gtaggcagcc tcctcgtcgg
     8221 cgggcgcaag cgtggggtcg tccacgcttg ccgcgcggta ccgcatccgg gtctcgcccg
     8281 tgccgcgcag gtcgaaagag accacgtcga agccggcgtc gaggcgggcg cgcagtgcgg
     8341 cccagtcggc aggggttgcc ttgccgtcga gggagaggtc gagcaggacc gggcgagcag
     8401 gggtagctgt ggggtgctct acgcctgtcg ggtgcacgtg cagcaggggc atcacgagcc
     8461 ctccgctgtg acgaaccacg taccggtcaa tggagatgcc gcccaccgcc gtcgtgcccc
     8521 tcgcctccca ggcaatcgcc gcgcgagggg tgacgcgatc ccacggcacc acgggccagg
     8581 cgtcgatcct cgggaacgag gcggagcggt agagcgcgcc cagcgtgccg gcgccgctcc
     8641 cgcgggtggc gcggtggtaa tccctgatga tctcgacgag cggccggtcg tcctcgagat
     8701 cgacgcgcac ctgccccgtg gcggtgcagc ggagcgcctc ggccggcagc ggcgtgaacg
     8761 gttcgaaccc gtggcgcgcg gacaggccgt tgaaccggtc gaggaaggcg aacacggcgt
     8821 cctggttctc gtccgagaag cggtgttcgt gatagccctc gaccatcgcg acgcggccgg
     8881 ccccgccgaa gcgccggtag atcgcctcga tctcgcggaa cgtgcggcgc gccccctcga
     8941 tcggcacgaa gtctttcacc gcggacgcga cgatgagcgg gcgcgggtgg acgagcagca
     9001 gcaggcccgg gtgatcgatg ccgcctgaca cgctgccgta ggggtcctgc tccgggtcgc
     9061 tgtcagggtc ctcgaagatg cggttcgcca tccgcatcgg cagcgacgtg atgtaacacg
     9121 acggcgcgac gacggcgatc cgctcgtcga gcgcgccgat ggtggccgac tggaagccgc
     9181 cgccgctggt gccggtgatc gccacccgcc gccggtccac ctcgggccgc gtcagcaggt
     9241 agtccagggc gcgaatgccg tcccaggcct cccaccgcgc caggttggct cccgcgaggc
     9301 aggcaaggtt gccgagcacg gcgtgctcgc cgcaaaccag gttgtagcgg ctgcgccccc
     9361 gcgcctcgtc ccagaactgg ctgcgctcac cctgcccaac cgggtcccag caaagcacga
     9421 cgtacccgcg gcgaaccagg cgcaccgcga tctcctggta gttgcggaac gccttcccca
     9481 ggggactgtg gccgcagggg agcagcaccg cgggccgcgg gcgtgcgggg ccgtccggca
     9541 gatacaggag cgcggggacg tgcaagccgg gcacgctctc gaagatcacc ttctcgatcc
     9601 ggtagccctc ggcgggaacc gtgccgacga ttctcgcgtt cagcggagtc cgttcgttcg
     9661 gcagaccgcc gatcgccgcg agcagccgct cgcgcgtgcg gcgcttcagc gcctcgaggt
     9721 cggcttcgtt ccgcaccgca gcccagtccg ccagccgctt tgaatcctgc gcccaggccc
     9781 gatccagctg gtagcggagg tacggggtga tccgcgggcc ggcgggcggc tgcgggtcga
     9841 gcacggtgaa cgcctcgacc ggcggcacct ccgaggcccg gcccgccccg ccgttcgcgc
     9901 cggccaggag cgccagccac tgcttcgcca tcagccggtg gtacggctcc gtcatgtgaa
     9961 tgccgtccag gcagaggaac cgcccacggt cggggctcgc ctcgagcagc cgccgcacct
    10021 ccaccacgat cgccccgctc gaccgcgcga ccgccgcctg cctcgccatc acttccggct
    10081 cccgatcgag cgggtcgagc acgtcgtcga agacgaagac gcgcgcgccg gcggccttca
    10141 gcccaccgac catggtcttc agggcctcga agtcctcctc ggtgcggttc gccacgacca
    10201 gcagcaccgt ctggggcttc caggcgagca cgtcctggta gaagcgcttc aaggcgcgcg
    10261 aggaagtgag gcccccaccc tggaagaagc ggaaggcgac cgccgggttc tcgcgctgga
    10321 acatcgcctc gacgatcggc gcgaacgccg agggcgacga ccagtgcagg tccatcgtga
    10381 acgagtggcc gatcacggca acccgctgcg ccggcatcgc gcgcatcccg gggatcagtc
    10441 tcgccacgtc cggctcctcg agcagctcgc ccgccagcac gtgcgcgtcg cgaacgcgaa
    10501 ggttcttctc cccccagttc cagatcttgc ggccagccag ctgccactcg gcggcggtga
    10561 ggccgaaggg cgaggcgcac gcgcgccctt ccaggcggcg cccggcctcg agcatcgccg
    10621 cgtccgaacc cgaaaggcca agcggcccca ggtacaccgg cagaccgagc gcgatgtaga
    10681 agcgatagag gtcctgcagc aggtcgccgt cgcgctcgac ctccagcacg gtctggtaga
    10741 gattggcgat ggcccgcagc gacagaggcg gcgcggcctt ctgccgcgac aggtcggcgg
    10801 cgaagacgtt caccggcagc cgctcgctca gcttcagtcc gctcgactcg agcggcgcga
    10861 ggtccttggc cgggtcgaac gaactggcct ggcccgcagc ccacgcggcc acctggtcgc
    10921 ggtcggaagc cagcagcgcg gcctggtgct cgaccagcgc ctcgagctgc gcgtcggtga
    10981 gcccctctcc cgcatagcac gcggcatcgc ccttcccgct tcgccgggcg agtagcacct
    11041 ggaaggcgtg cacgccggca atgttggcct cgacgaggcg cgccagccgc tcgcggcccg
    11101 tcgtcacgtc ctccgtggag gtggcatcgc cggccgccgg gccgtgccac acgccaccgc
    11161 cgaaggtggt cacgtagatc gaggacggat cggccgggtc gatcacgaca cggtggcccc
    11221 acttgaagtt gaagccggga acccggtgcc aggtctcgcc gcggtccacc gaccggagaa
    11281 cggcctggtc gaagccgcac gcgtacatga cgtcggggtt gcgcgggtcg atcgtgacgt
    11341 cgtacacgtg cccggtggcc ccacgggcgc ggcgccaggt tccgccgcca tcgtcgctga
    11401 tgaagatgcc gccgccggtg tcgccgccag gggtggcgac ggcccaggcc gacagataca
    11461 ggcgccgcgg ctcgcgggga tcgatggcca ggccgttggg cccgttgacg cccggcggaa
    11521 ggtcgagctt cctccagtgg tccgcgccgt cactcgacac gtagagcgcg ccgtcgtcgg
    11581 cgttgccgat gcggccgcgc tcgctgcgtc ttgcgacgac gaggtagagc aggccggccg
    11641 gatcctgcgc gagccgccac gcgaacggct gttcgcgggg gtcgggcgcg aggccggcgt
    11701 tcttcagcgt ccacgtccgc ccgttgtcga tcgacttgta gacgccgcgg ccgaacgcgg
    11761 cagcgtagag ggtgcgctgc cccttcgggc tgcggggatc caggatgagg tgcgtgacgg
    11821 cggattccgc catggcgccg ttcgacggcg tccagttccg tccaccgtcg gtggaaacgg
    11881 caacgccgcc gcggaagctt gccggatcgg tgcgccgcca catcttgggc cgcggcaggt
    11941 cgtgggtgcc gctgaacccg ccccagacga gccccttcac ctcggggtcg aagacgaccc
    12001 agtaggccgt gttgcgccag ggccgcggga tgccggagct cgagccaatc caggtcgcgc
    12061 ctccgtcctc gctgccgaag agcccgatgt cggtggtcgc caccagcacg tgtttcggat
    12121 cgaacggatc ccacgccatc ccgtagtggg tcgtgacgtc gagaccgcgg ctgacccagg
    12181 cgtcgccgtt ggcgcgcgcc gagttcacct gggcccacgt gcggccgccg tcggtggtgc
    12241 ggtaggtgcg gaacaggtcg gtgacgtggc agacgagcgg atcggagggg gcggcgccga
    12301 tgtcgtacgg cgcgtcgagc cacaccgagc ggccgtcctc gaccgcgcgc gcctcgatcc
    12361 acgacggctc gaagttggcg gccggccggt cggattcgcg gtgcacgatt gtccacgtgc
    12421 gcccgccatc ggcggtcctc gcgacaccgt tgaacggctt gccatcgccg tccttcgcgc
    12481 ggagcccccg aaggccgacg taggcggtca ggccgtggcg ggcagaggcg ctcaccgggc
    12541 ccaggcgcgg ccgggaggtg tgtgccggcc cccaggtctc gccgcgccca actgcgtcgc
    12601 cgaacagctc gctcagcctg ccgttggcct ccacccagtg ggcgcccgaa tcgcgtgaga
    12661 cgtacatgcc gccgcgaact cccgcctcgc ccgcttccag cggcgccgtc gcgtagacga
    12721 gagtggcgcc aacttcggca tcgcggccca cgctgccgga atcgaagcgg actcccgccg
    12781 gggccgggcc gcgccgccac ctgtcgccgg ccctgacgaa gacgccttcc tcggcaagca
    12841 gctgcacggg cccaccggcg atgccggccc agatcgcgaa cacccgctgc gccgggagat
    12901 cgccgatcgc cgtccacgtg cggccgcgat tcgcggatgc cagcaccgtg gtcgtctcgg
    12961 gctccgagct tccgggcggc ccgggtttcc tcgcctggat cgcggcgaac aggcggttcg
    13021 agtccgactc gtccacgccg atcgcgtgga tgacgacgtc ctgtccgctg ggatagagcg
    13081 ggtcgtcggt cgtgtagacg gtttcggcgt gatcgcccca cccgttggcg accgtgttcc
    13141 gcgcgggatc gggaaacacc atcgcccagg tcttcccccg gtcgtcgctg cggaagatcg
    13201 ccgaggcggc cgcgtagatg acggagggat cgttcgggtc gaaggcgaag gcgctcgccc
    13261 cggtgccgag gttgaacatg cgccacgaca cgccgccgtc caccgtgagg tacgacccgg
    13321 tcatgtcgca ggtctgggcc gccacgttcg agtcgtgcgg gctgatcacg gggtggagcg
    13381 tcgtgccccc gccgccgggt ccgacgacgc gccaccggtc ggttcgcgcc gagcgggcaa
    13441 tgctggcgcc ctgctccacc gctgagacat cggtcctttg cgccgcaacc ggcgccaggc
    13501 aggctaacgc gcacagaagc gcaaggaggg ttcgtctcgc cggcaccggc atcatgaccg
    13561 ccattcctcc cttcgcgcgc gtatcgcctc ggccacctcg accgactgcc ggaccgcttc
    13621 gagcgagggc cgcgggggaa gcccggccgc gaggctggcg aagaacgcct cgtactcgcc
    13681 gtagaagccg ccgagcacca ccgggccggc gccggccgcg ccgcgcacgt cctcgacgac
    13741 cgtggcccgc tcgaggtgca cgaggcggcc aggcgtgtcg tatccgcccc acatcgggat
    13801 ctccacgaac agcgtgtggt cgcgcgcgtg cagctcggcc cgctccacca cgacgcctgc
    13861 aaccgggcag cacgcgatgt gcgcggtcac gccggatcga agcaccgcgt ccatgaagat
    13921 gttggcgacg ccggggccca gcccgggcag cggctggtag cggaagcgga tctccccgta
    13981 gtcgctgccg gcgatgaacc gcacggcgtc gatcgcgtgg atggcggtgg tcgagaagtc
    14041 ggggtcgcgc cggttcaccc ggaccatctc gtagcggatg tgctggagcg gcgccgaatc
    14101 aggcagctgc tcgacgcgct cgcgcagccg gacgaccagc ggagcgtagc ggcggttgaa
    14161 cgccacctgg tgcgggatcg cgcgcccgtc gcggccgcgt tcacgcgcgg cggcggccag
    14221 gcgatccacc tcgcccaccc tctcgcccgg gggcttctcg agcagcagcg ggatgccgcg
    14281 cgagagaacc cgcgtgccaa catggcaggc gaaccggacc ggcacgacca ccgcgaccgc
    14341 gtcgggccgc tcggcgtcga gcatcgagtc gaggtcggcg tacgcgcggg cgaagtggaa
    14401 acgctcgcgg aacgcttccg ccttcgcggc atcggtgtcg cagcaccccg ccagctccag
    14461 gccggggtgc gaggcggcgt agagagcaag tgacggaccg tgcgagctga cggcgtgctc
    14521 gccgcagccg atcaggcaga atcgcatggc cgcctcactg gccctcgacc gaccgggccc
    14581 ccgtgtccac gacctcgaag tcggacccct tgatttgcga gagcgtctcg agccccttca
    14641 aacgccggtc gggcgcgatc gacatgtgcc agtcgctgcc gttgtcggcc acgaacatgc
    14701 cgtacttctt cagccccttg aggatggcct ggacgtgcgg cgggaagggg gtcacgtcga
    14761 agtccttccg caggcggaac cgctccccca tccgcggcag cgcggggtcg gtctcgcggc
    14821 tggcccagtg ggtggccggc agcacgtagg cgcgccgcgt ccggcggacg gtgaagcgca
    14881 tcgcgtgctc gaccatcccg cgctcgcact cgtcgtagcg gacgacggcc gggaagatcg
    14941 gcaggccggc ggcgtcggcg gaggtccagc gctcggggcg gagcgccccg gtcttcaggt
    15001 cgaaggtcgc gttgttcgac gcctcccagc cggcgtcggt ctttcgcgcc tgccagaact
    15061 cgtagagctt gccgttcccc gggtccacca cgatgatgtg gcggtcgccg gttccctcgc
    15121 gctgcagctg ctcgagcgtc tgaccgggct tcggcagcgc gtcgacgtcc tcgttgcgct
    15181 gcatcggcca gttctcgatc ggggcgttgt cgggcaccgg gtagggtccc gggtccgact
    15241 cctccgggta gtcgagcagc ttcaccggca cccgcggctg gttcgggggc acgatgatga
    15301 accccatgtc gagattgtgc tgcagcggct tgtcggggcc gatgatggcg atcatcttct
    15361 gcgagtcggg gtggacgggc agcgccgaga tgtcctggtt ccacgggttg tccgggggga
    15421 acacctgcag cgcggtcagg atccggtcgg cttccggcgt gtcgaacatg accggctcgg
    15481 tgattgccgg catcgggggg ggagactgct gcgccggcgc gttcgcgcag gcacccgcgg
    15541 cggcggcggc ggcagacagg cagagaacca cgagatggga acgggtcgtt gagaacatga
    15601 agactgctcc ggtgacgggc tacatgggcc gaagccgcag gcggacgccc cgccgttctc
    15661 cgagatccac tcgcgcagtc cctgcggcgc tggcctgcgt gagaagacgg ccggcaggag
    15721 atcccggcgc gaagttcgac gtcagctgca cctcgtagga gcggtgcggc tcgaggcccg
    15781 cgagcacgag tgccggtgcg gcgagatcgg gcagggtgac ctcggccgcc gccgggaatc
    15841 ccggcgacgt gacgaacaac gccccgccgt gaccctcgac aaccgcgccg gcaaggttgt
    15901 gcccgcggac ggcctcgatg cggcgcggcg gcgcgccgcg gtcgccgatc tcgagcacgt
    15961 gcaggaaccg atcgtcccgc gcggggacgg cgggggtgac ctcgaggcgc caggctccag
    16021 gcaccaccga ccggcggttc gagggcgaca gccgctcgaa atcaggcccc cagaacgtct
    16081 tccacatccg gcgcagcacg gggtcggcgg ggagcgcgcc gccttccggc ggatccagcg
    16141 gccagttggc gccggtgccc cactcgccgc ccttctcgtc gcccggcgtc cagaactccc
    16201 agcccgggcc gccgcgcacg atcacctcgc gggactgcgg aaggacggcg tgcacccgca
    16261 ggcggccgcc gccttcctcg aaggtcgcga ggctggcgtt cggccaggac gtcccgccgt
    16321 ggcccacgtc gcgctgcgga ccgtcggctc cttctaccgc cggggcgttc acgccgtgca
    16381 gcagccaggc tttcctgtac gagggatcga ccgaccgcac gtcgtccgac acgaagagca
    16441 cgtcggcggc ctcgagccag agcaggtcgc ggacgaaccg ccgcagcttc gagggctggt
    16501 aggccgcggt gccgtcgccg cgcacgtagg tgtaggcgcc gggccgcgcc tcgtaggcgt
    16561 cgatgcgccc ccggtcccag aggtcgcgcg tccgttccca gtcctcgagg ctgcgcaccg
    16621 agttccagaa gcgcgacgag tccatgcgct ggccgccgtc gttggcggca ggccagaggt
    16681 tctcgcccca gaagaacgtc tcgcccggct ggtagacgag gatcgtgttg tgggcgacgg
    16741 tgcgccggta gtagttcagg taatgggggc tctcggtgtc ggtatagtcg gcgccgctgt
    16801 cgagcgcgag gtgccccttg cggaagatcg cgaagtggtt ggtgtcgagg tggtcgtgct
    16861 tcgcgaagta gggcccgcac gagaactcga tccaggtgtc gccaggctcc cacccgccgc
    16921 gcatcacgag gtggttgatg cccgggaaca ggcgctggcg cggcagctct tcgggacgcg
    16981 ccaggcgcgg atcgcgcggc gccaccgagc ggtcgctcca gaggaactcg agcaccggga
    17041 gccgccactc cttcggcagc cagccgctct gctgcaggaa ccatgcagcg tacggatcct
    17101 tgaaccggtg caccgcgtac ccgagcacca cggtgtcaac cgcgtccagg ggcgggaact
    17161 cgttgtcgtc gtcgcgcgcc gccgagccgt cgggcagcac cttgtagagg tagccgggcc
    17221 ccatgttcgc aaagacgccc cagcggcgcg cggggtcctc gccggtggtc gtccgcctca
    17281 actccgcgag catggccagc ggcgtgaagg tgatccgcat gtagtccatc gactcgtggt
    17341 agccgccgga gggcgcgacc agctccgagg tctcgagcat gttgtcgagc gcccgtcccg
    17401 cgtgcgccag cagcagcgac gcccgtgcgg cggttggcgg gtggcgctcg gcggctgcca
    17461 gcgcgaaggt ggcgagtgtc agctccctga tcgagtggtt gtggaacgac gcgctggcgg
    17521 gatccgcgag cgactgcagc gcgagcatct gctcggcgcc cgcgacaagc tcctgcgcga
    17581 cctggtcttt cagccccgcg tcgaacagcg gcgagccgta cagccagtcg aaggccaggg
    17641 ctcgcgcgac gtactggaag tacggccttg agttgcgcgg caccggcagc ctcacgcgcc
    17701 gcatctcgtc caccgcccgg cgcgcgaacg attcgtcttt tgtcagcaac caggtcaacg
    17761 cccagcctgg caggtcgtcg gacgggcgct cgcctgcggc atacttcgcc tcgagcgccg
    17821 ccaggctggt gaagggatcg gaagcagcca gcagcatccg cggccggtcg ttggcgccgg
    17881 ccacggccag ccagcatgcg caaacgccca gcaccagcag gctcgagcag cgtctcatcc
    17941 cgcctccttc agcggccggc ggcaggcgtc acctcgaagg gcgtctcgcg cacgaccgcg
    18001 gtgtgtccga cctcgtcggc aatctcgacg cgggccacgt agcgcccggg cgagaacggc
    18061 gccagcgaga tggggccgac cgacggcgcc gcgccggggg tgtcgaacgc ctgctcgctg
    18121 ctcttcgcga ccgccgcccc gtcgcgaagg acgctgaagg acgccttgat cgacgcgcgc
    18181 ccggttgccg ggtcgagccg cgcgttgtgg accagcacca gcaggcgcag cgacccggcc
    18241 tggtcgagca cgttgccggc ccgcggccgc aggcgctgcg acccgatggc aaacgccgca
    18301 tacgggtcgg ggcccgccgg cgccgccgtc gcctcctcga ggcccgcgag aaccgcgagc
    18361 gggctgacga cgagcgagct gccggcgtag tcgggcgact ccacgtcgag ggtggtcgtc
    18421 gcccctcgtc cggtcgcagg gtcgagcacc gccaaggtca ccgcgaatcg ccccggaggc
    18481 agtgacagac cgtgggaact gacgaacgac ccgtcgggcg cgacctcggc aaccacctcg
    18541 cgctcgtcgc ccggcaccgc cgaaccggat gagccggccg cgcgcgcgat ggccttcagg
    18601 cggacgaact tctcactctc gggcacgggc agcgagcccg gggcaccgcg gagcaggccc
    18661 gcgacgtacg agccggcctg ggccggcacc tggatcttca actcgaacga gagggtgaaa
    18721 tcggcggagg gccggtcgag cagcgcggcc gccccggagg cgggcggcgc cgccagcggt
    18781 cgcaggcggc cgtcggcgcc gaactcgtag ccgatctccg gatgcaggat cagcccggcc
    18841 gctatccggg ccagctcgtc cagcgtccgg gcgctctcct cgaactcgca cccgtcgtcg
    18901 aactgcagct tcaggtcgcc gccgggcatg cggaaggttc gggcggggct gctcttgtag
    18961 acccacagct cggggcttct cgcgccgtcg cgcgtggcgt tgcgcgacac cggctcgcgg
    19021 gtgcggtacc ggctcggggc ggatggcctg gtgtcgaaga tgttgcgcag ctcgcggccg
    19081 gtgacctcgt cggggtgccc gagcagcatg acgacctggc cgcaggccgt caccgatccc
    19141 ttcttgccgg cctcgccgaa ccgggcgtcg gcgtcggccc gcgcccggtc ggccgcggca
    19201 cggaagcggt tctcgggcga gccggaggcc gggtcgcgcc gcgcccagaa gatgcggacg
    19261 aacgtctcga ggtcgtcggc cttcacccgc cgcaagacct cgacctcctc cggcacgagg
    19321 aggaggcggt gctgatcgaa ccactcgcgc acgggcggct cgagacgctg ggcggccgcg
    19381 ggcgccgcaa ggagcgacgc gacggccgcc agcaggatgg tcttcagcgt tcgcatggtg
    19441 gaggaggggg tggggcaggc tcgaaggcct gccccacgct gttgggatcg cggcgcgggc
    19501 gaaagacccg cgccaccgct cagaatcgca tgcgcagccc gaggcgtatc tgtcgtgaca
    19561 gcgcgaagcc ctcgacgttg gcctcggctg tggagccgag cacaccagtc gtgccgttgt
    19621 cctcgatccc gttgacgagg tagtcggtca tgtcgaggaa ctcgctgccc ggcccgggga
    19681 agaactgctt gcggttgaac gcgttgtcca gcaggcaggt gaactgcagc ttcgcgccgc
    19741 gtatggacac gacgtccttg tagaaggcga ggttgacgat ccagttgccc ggtccctcga
    19801 ggctgttgcg cttcgtgttg cccgggaact tgcccggcgg cggcatctcg taggcgccgg
    19861 ggttgaagaa catcccgctg ggcacgttcg ccttcgggtc gccgatctgg tcgccgcggt
    19921 agctgtcgcc cacccagacg cccgtcgtgt cggggttgaa gccgatgttg tacggcgtga
    19981 acgacgagct gaggacgaag tacggggtga ggttcgcgcc gctgcgcgcc tggaagatcg
    20041 ccgacacggt ccacccgccg aagagagcat cggcccaggc gggcatgtcg gcggcgtagg
    20101 cgcggccccg gccgaccggc acgtccaccg tcgcgttcaa gatgaggcgg tgcttgacga
    20161 cgtacgggtc gggtccgcgg tcagcctcga gattgtacgg gtcgtactgc aggacgccca
    20221 ggctcgagtt gcccgagtcg ggggcgttgc tgctcgaggt ggcgtaggtg taggccagat
    20281 ccaaggcgaa gccgttgcgg aagcgccggg tcagctcgag ctgggccgcg ttgaagcgtc
    20341 ccgacccggt gttctcgatc atgttcacgt acggaccata catcgggaac ggccggcggt
    20401 agttgtacga gggatcccag ttgtagagat cctcggtgct cggcttgacg ctgttgtact
    20461 cgcggttgac gagcagcttc cgcatcgtcg atccgaggta gctcacccgg acgcccatgt
    20521 tccagggcag ctcgcgctcc agcgtgacgt tgtactggta aatatcgggt gactgcacgt
    20581 cgggccagac accctgcgtg ccgaagtcgt cgttgccggg catgcccacg ccctggctga
    20641 aggcgttcgc gaaggggccg ggctgccgct ggatggtgta gcggaactcg ttggtggcga
    20701 gctggtcgcg aatgccctgg atggcggtcg tcgggtggaa gagcccgaac ccgccgcgca
    20761 gcaccgttcg cccctgcccg tcgaggcggt acgcgaaacc gacgcgcggg ctgaagttgt
    20821 tcgtgtcggt cttgatgagc gtcttgggaa gcgagaggtc gcccgcgagc ttcacgtggc
    20881 tgtagtaggg gctgtcggga ttgctcaggc cgtaggggag cagcgaccag accgtctggt
    20941 cggcgaccac gtggtacccg ccgtccttcg cctggaagtt ggcgagcatg ttgttcttct
    21001 cgttccagcc gccggccagc tcgtagcgca ggccgaggaa gagcgtgagg tccttgttga
    21061 cccgccagtc gtcctgcacg aaggtggcga agtcccacga gtagccgttc aggtcgccac
    21121 gggtgctgac gtgttcgtcc actcgcgtgg gcatcccgag caggaagtcg gcgaacgcgt
    21181 tgccggtgaa cccgccgttg aagcggtacg cgccgcggtg gttgaggccg cgcccgaagc
    21241 cgtcgatcgc gctgttcctg ccgatcagca cgccgccgcg gagcgagtga ccgcccgcca
    21301 tccacgagat gttgtccgcg atcgagaagg agttctgctc gatcgtgcgg ttgttgttgc
    21361 cggtgaacct gattttttgg agagacgctt caa
//
LOCUS       DAOPQX010000018        21211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3087_length_21211_cov_4.266686, whole genome shotgun sequence.
ACCESSION   DAOPQX010000018 DAOPQX010000000
VERSION     DAOPQX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 21211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21211
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3087_length_21211_cov_4.266686"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="PKK95_01955"
     CDS             complement(<1..870)
                     /locus_tag="PKK95_01955"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOC16997.1"
                     /translation="MKRASCLVTALLLLALAFAGAASAQTQTGTVLGRVMDQQGAVLP
                     GVTVTLTGPRGVQTAVSDAEGNYRFVGVQPATYTLKAELAGFTPQQEDRVIVTMGRTL
                     TLDFSLKVGGLTETVEVTGTASTVDVKSSATTTNVSGDLLSSIALYDSTATGLMNAAP
                     GINDESAFGGQDYYGNALLLDGVDTRDPEGGSAWTFFNQNLIEDIQIGGLGVPAEYGG
                     FSGAVVNTITKSGGNTFSGLFSMRYTRGAWAATNVTDEIIDQNPSLGEGGDVLRKLTD
                     YTVQMGGPFKKDRA"
     gene            complement(882..1208)
                     /locus_tag="PKK95_01960"
     CDS             complement(882..1208)
                     /locus_tag="PKK95_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC16998.1"
                     /translation="MSVLLRRRHCGRIFALALAVALLPLPVLADEGPAAVKPGIRVSA
                     AAIATSEPLAATAAPAAQSGPSDAGSVSFFKKPVGIAALIVFAAGVGYAVYSTQNDRI
                     SSPAKK"
     gene            complement(1359..3758)
                     /locus_tag="PKK95_01965"
     CDS             complement(1359..3758)
                     /locus_tag="PKK95_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC16999.1"
                     /translation="MPRPEDARPAPPANPSSSPRLWLRAGAVVAVMALAAAGAAWWWA
                     GRRPQETAAGVALGAATGGGRASELNLLVITLDTTRADRIGAYGYAGARTPAIDRLAR
                     EGVVFEQAMTDAPLTLPAHCSIFTSRHPPRHGVRDNGGFFLSPRQRTLATELKARGLR
                     TGAVVGAFVLDGKWGLDQGFDFYGDDFELDRPAGRGFSVGEIQRRGADVVDKALPWLE
                     QVKGERFFAWLHFYDPHTPYDAPEPFASRFPGHPYDAEIAYTDAQVARVVEFLERDAL
                     LDRTVIVVVGDHGEGLNQHDEGTHGFFIYESTTRVPFIVRAPFERTRGRRVADPVRTV
                     DLMPTALDLLGIPAPEGIDGVSLAPLMTGDRPTLDLEGYAEALYPLHHYGWSDLRAWR
                     SGRYKVIDAPRPELYDLERDPNETANLYAERQALADGMIRRLRERQPEIESDETVARP
                     APEVDPEARARLAALGYVGSFVATLAGPRSERADPKDKIGVFNLMGEARDLAQQEGGF
                     EKVVALYRKVLDEDPNVVDAWFNLGNVHFRQRRYEDAIGYFKRTLELKPDYDLPVINM
                     ANAYRQLGQDEAALAGYEHYLTIDPKNAPVRYQMGEIYLDRGDVAQAEENFRKALEID
                     PREASARNALGVIAFQRGDLAAAERETRAALAIRPTVRLAHYNLALIAEERQDWQTAL
                     AEYRREIELHKNAYRAAFNLGRLHERLGQRADMVAALEQSVQMNARFAEGRIFLAKAF
                     LEDGVRFQEAIDLAREGLALQPRRDVAQLGHYVLADLYNRVGRHADAVREAALGEKLG
                     R"
     gene            4081..4386
                     /locus_tag="PKK95_01970"
     CDS             4081..4386
                     /locus_tag="PKK95_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17000.1"
                     /translation="MRLLALALAVTVLPLPVSAGQQDQTTPKPGIRASIAKAVAVEPP
                     AASKDAVAVVQDDPGAKLNSPSFFKTPAGVAVIAIIAAGTGFAIYSASNDRIKSPGR"
     gene            4403..7126
                     /locus_tag="PKK95_01975"
     CDS             4403..7126
                     /locus_tag="PKK95_01975"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC17001.1"
                     /translation="MKRILMGLALIAALMACADAAVAQSLTGTIAGRVADEQGGVLPG
                     VTVTLTGRTGSQTQVTDERGEFRFIALEPGIYSVKAELEGFRAKEEANIQVGIGRTAD
                     VRLALGVGGLSETVEVTANAVTIDTTTTATDNTISKDLLFAMPLTQANPSVNLLDYTP
                     GVNDNSAFGGTGSYGNALLLDGVDTRDPAGGSSWTFYGFNMIEEVQVAGLGQPAEYGG
                     FTGAVVNTITKSGGNRFSSLLEYRYTNKDLAGDNTSDAIAAINPGLATPSRWDKMNDY
                     TVQLGGPLKKDRLFFFGSVQRYSIKEDPDGPRTVRTEVSPRFNFKLTANLTSSDTLSF
                     GGQYDWYNQTGRVAWGPTAAATDDRTSDQDSPEAVWNAQYRKVFGSSTFLEAKFTGWW
                     GYYYVDPIKPGAAHYDVDSGAYSGGGGEYFWADRGRNQVNVSLSKFVQKAGQHNFKFG
                     VEIERSRVRDRYAYAEEHGRPIAYYDYAGEPYMAYSYSYDLQGRNQRESFFAQDQWKI
                     GNVTANLGVRLDNIRGRSPVLDETLYDTKSLAPRVGLAWDVTGKGESVLRAFYGHLYE
                     GPQYYTFSYALPGIGDFVGYEVLPGWQLGEEIFRVPGTSKYSMGKDLKHPRVDEFNVS
                     YQQQFLRNYKFTATYVRRDTKNVIDSVFPEARWEPFSYTNAKGQQMTLYRWANDEIDQ
                     KFEVRNVDGFQFLSPSGQVLGTIDAFRKYDGMMFVLQRALRNRWQAQVSYVYSRTKGT
                     VDNDGSDGLGGTNFQNPNTILVNRNGYVSNDRTHEVKVFAGYQIPVIEVGVSGYLSVI
                     SGYPYQPYERLTGSRINWTGAKNVFIESRGSYRTDAITAFDFRAEKVFEFAPYRFGLY
                     MDARNLFNQGTVTWVQTRYPSAEISGAAVPFGGPVYVQSGRQITFGGRFSF"
     gene            7301..8248
                     /locus_tag="PKK95_01980"
     CDS             7301..8248
                     /locus_tag="PKK95_01980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOC17002.1"
                     /translation="MKTWTIGEADGGARLDAWLARQPEIGSRGRARSAIERGKVFLNG
                     EGQDFADAATRLSPGDQVGYWEDRPGSARTPARDVVSARAALRIVHEDAAILAVDKQP
                     GWLVEPLPGEEGSEVTLLDLLRDHLRAARRRPFVVHRIDRDTSGLVLFALTPAARDDL
                     KRQFERRTPERIYLAVVHGLVVPASGTWRDHLVWDTERLIQRRAHIHEERAREAVARY
                     RVLEQHEGGAVVEVSLVTGKRNQIRVQAGLRGYPLVGERLYTYGRQGEPGPRLELGRQ
                     ALHAATLAFRHPEDHRPVQLDAPLPDDLRNLIDRLRAGS"
     gene            8339..9805
                     /gene="glgA"
                     /locus_tag="PKK95_01985"
     CDS             8339..9805
                     /gene="glgA"
                     /locus_tag="PKK95_01985"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOC17003.1"
                     /translation="MPSRQILMIASEAVPFAKTGGLGDVAGALPAALARQGCSVTLIV
                     PRYRAVAGGTTITRFPVTVGGRTLDVGFVEHPLAGAARAVLVDCPELFDRPELYGVGN
                     ADYPDNAFRFGAFTRAALEFAARLDLHPDIVHAHDWQSGLAPVYIRTLYANHPELARA
                     GLVFTIHNLAYQGLFPPHWLPALDLGWDLFGIDGLEFWGKVSFLKAGVNFSDVITTVS
                     ARYAKEIQTPEYGFGFDGILRRRADRLVGILNGIDTAVWDPASDPHLPEPYDADRLER
                     KRSSKRALLEAVGLPSDDQALARPLVGMVSRLVDQKGFDLLALLGERLLALDASFVLL
                     GSGEPQYQEQWQGLAARAPSRVAVRIGFNEPLAHLIEAGADIFLMPSRFEPCGLNQMY
                     SLRYGTVPVVRATGGLDDTIVNWSPRTGRGTGFKFADATPEALLAALRKALEVYRDPS
                     AWRSLQLQGMACDYSWDASARHYGTVYENAIRARAKRA"
     gene            9888..10217
                     /gene="trxA"
                     /locus_tag="PKK95_01990"
     CDS             9888..10217
                     /gene="trxA"
                     /locus_tag="PKK95_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC17004.1"
                     /translation="MAAAAIQQVTDSTFEQLVKSSTPTLIDFWAEWCMPCRRVAPVVD
                     ALATEYAGRLNVAKMNVDENQNVPIRLGIQSIPTLMIFKDGNLVDMHVGATDKDVLRK
                     MVDKHVK"
     gene            10237..11211
                     /gene="trxB"
                     /locus_tag="PKK95_01995"
     CDS             10237..11211
                     /gene="trxB"
                     /locus_tag="PKK95_01995"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOC17005.1"
                     /translation="MNEVRDVIIIGSGPAGLTAAVYAARANLRPLLIEGAEAGGQLML
                     TTTVENYPGFADGILGPELIGAMRSQAERFGTEFVQANVESVELSSPPYVVKTADEEY
                     RCRTLIVATGASARLLGLPSERHLMGHGVSTCATCDGFFFRGKPIAVVGGGDSALEEA
                     NFLTRFASKVTVIHRRNALRASKIMQEKAFENPKIEFRWNSVVEEVKDVTRGQVTSLV
                     LRDVLTGELSELRVDGLFVAIGHTPNTSLFAGQLKMDANGYLITHDGTLTNVPGVFAC
                     GDVQDHVYRQAITAAGSGCKAALDAERYLEHVMVEGLGREVGDTLKPA"
     gene            complement(11221..12285)
                     /gene="mnmA"
                     /locus_tag="PKK95_02000"
     CDS             complement(11221..12285)
                     /gene="mnmA"
                     /locus_tag="PKK95_02000"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOC17006.1"
                     /translation="MTRVVAAMSGGVDSSVTAALLVERGYEVIGVSMQVYDQREGASG
                     FGSCCSLDDLHDARRVAAAIGIPHYVLNLQRRFQETVIDNFVAEYLAGRTPLPCAHCN
                     TDLKFAELVGRADLFGAEAVATGHYARIERDDDAGRYRLLRGVDASKDQSYFLFGLTQ
                     AQLARAMFPLGGLEKTEVRRIARSLGLAVADKRDSQEICFVPGGDYAAVVERAAPGVT
                     GGEIVDREGRVLGRHGGIHRFTVGQRKGLNLSVGVPLYVIGVSSGTGQVMVGPREALE
                     RRSLSAARVNWVSGRQPASPATLHVQIRSRHQAAPALVSPEGDAGARADFHEPQAAIT
                     PGQAAVFYDGDEVVGGGWIE"
     gene            complement(12366..13508)
                     /locus_tag="PKK95_02005"
     CDS             complement(12366..13508)
                     /locus_tag="PKK95_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOC17007.1"
                     /translation="MVPVYLDHNATTPVAPEVAEAVASALHDTFGNASSLHAPGQRAR
                     RALDEARQAVAALVGADPAGIVFTSGGTESDNLAVRGALEAAPATRRRLVVGAVEHEA
                     VLNTALALQRRGWPVTIVPVTSDGVVTPEALAPHLGPDVALVSVMLANNETGVVQPVA
                     ALASLAHAAGARFHTDAVQAIGKIEVDVRELDVDLLSLTGHKFGGPKGAGALWIRRGV
                     PMAGVLTGGRQERNRRPGTENVPGIVGLGVAAELAAGSRNKRAAVAGRRDRLERAVLA
                     AVPGSVVNGAGAGRVPNTTNISFPGAEAESLLIALDLEGIAVSSGSACSSGTLEPSHV
                     LRAMGLPPARVQSAIRFSLGPATTDEDIDRVLAVLPGVVERIRRVL"
     gene            13842..14258
                     /locus_tag="PKK95_02010"
     CDS             13842..14258
                     /locus_tag="PKK95_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOC17008.1"
                     /translation="METRDVVNIGKSVVIKGELNGSEDLTVEGHVEGRIELKDHALTI
                     GPHGRIKAEIHAKSVIILGEVTGNIIASEKVDIRDNGSVDGDIVAPRVAIAEGAHFRG
                     SVDMQRKSAPTPPPAPKAEAKPAPPAAPGQPAKAGV"
     gene            14401..15063
                     /locus_tag="PKK95_02015"
     CDS             14401..15063
                     /locus_tag="PKK95_02015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC17009.1"
                     /translation="MTGFLSRFGQRRGADPDDVPETAGAPDTVVGTKALRKFISYLKS
                     RPTPVLLDLGPVVGSNISFFGEQIGCKIFVEDLFADIDRHEREGRLADLPAFLRSRFP
                     QASGSVDGVLCWDLFDFLDRSAAQAAAEQLSRVLAVNGALFGFFGMTRPSEVRLTKFV
                     VVDDANLRHRPYSIPRMRQAVLQNRDITRLFEPLRVSDSFLLKNNVREILFRKPDYMD
                     SR"
     gene            15184..15990
                     /locus_tag="PKK95_02020"
     CDS             15184..15990
                     /locus_tag="PKK95_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOC17010.1"
                     /translation="MRPIVALLTDFGVRDHYAGTMKGVILGICPEATLVDISHDVPPH
                     DLRTASLELAASYKYFPPGTVFLVVVDPGVGSGRRPLAAEAGEYRFVAPDNGVLTAVL
                     KETPPRRVVELTERRYGRPTVSRTFEGRDRFAPAAAWLAKGVEVRALGRPLADYTLLD
                     IPDAEVGTDAIVGAVLRVDRFGNLITNIDRRSFDRLARLGGRLHIRVGEADVAQLVST
                     YAEVPAGEVAGLFGSSDHLELAANSASAAGLLGAGPDAVVTITRIAEEQV"
     gene            15992..17200
                     /locus_tag="PKK95_02025"
     CDS             15992..17200
                     /locus_tag="PKK95_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOC17011.1"
                     /translation="MDFDLGEEHLLLEQTVREWAERAVAPRIRELDREHRFDASILAQ
                     MADLGLLGVSVPSSYGGTGTDYISLGVASEELEYVDTSLRVILSVHVGLNSLTLLAWG
                     TEAQKQRYLVPQAQGKKIATFGLTEPSAGSDVRGIQSVAVKHGDRYRLTGEKQWISLA
                     DVADHFLVFAWTDLEKKKQRDPSGLTAFIVERIFPGFSSGTLKEKWGILAGNTGFFRM
                     EEVEVPEENVVGRPGEGFKIAMFALDQGRYTVAAGATGLIRACRDASVKYAKERRAFG
                     VEIARHQLVKAMIAGMESDYQAARLLWLRAGWLKNLGRRNTRETSLAKWFATVASERA
                     AGNAVQVHGANGYSDDYPVGRFYRNCKGAVIYEGTREIHQLMQADYLLGYREDRTTRC
                     TLPPVGSGDT"
     gene            17300..18289
                     /gene="glpX"
                     /locus_tag="PKK95_02030"
     CDS             17300..18289
                     /gene="glpX"
                     /locus_tag="PKK95_02030"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOC17012.1"
                     /translation="MVTNLSLEFLRVVERAAIACAHTMGQGDGPRADRAAVEAMRREM
                     DVLPIDGTVVIGEGERDEAPMLYIGERVGQAHLRGAAGGAFDKVDIAVDPLEGTNLCA
                     VGMPGAISVLAASGPGGLLHAPDLYMEKLIVGPKSKQAVDLDAPVADNLSAIARCLER
                     EVGDLVVVVMDRPRHERLIADIRRAGARIRLITDGDLSAGIAAAVVGSGVHAVMGIGG
                     APEGVLTAAAMRCLNGEIFGRLVIRTAEDERRCREMGIEDVRRVYRAADLAPGGSVIF
                     AATGVTDGTLMRGVRFFGDGIRTSSLVMQTDPHLIRFIDSIYVQYGQDVRIRF"
     gene            complement(18293..18427)
                     /locus_tag="PKK95_02035"
     CDS             complement(18293..18427)
                     /locus_tag="PKK95_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17013.1"
                     /translation="MKRAPRTRKPKLPRVPLPRQVGGAHEDRRKRPARKRKHKGQDEG
                     "
     gene            18533..19165
                     /locus_tag="PKK95_02040"
     CDS             18533..19165
                     /locus_tag="PKK95_02040"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOC17014.1"
                     /translation="MRTWTVNPSRPDESVLAEAAAVVRQGGVVVFPTDTLYGLAADPC
                     DARAVDKVFAVKGRAGREPLPLIVAGLEEAETIARMTTLARRLAAVFWPGPLTLVVDD
                     RGIVAPAAHAGTGTIGMRVPASPVAAGLARLAGGVVTSTSANRSGAPAPVSAAQAWDG
                     LGEAVDGLLDGGPAPGGRPSTIVDARGERPVMLRAGAIEWERVVEALERR"
     gene            19162..20445
                     /gene="hflX"
                     /locus_tag="PKK95_02045"
     CDS             19162..20445
                     /gene="hflX"
                     /locus_tag="PKK95_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOC17015.1"
                     /translation="MTAKRQREAGERAALVGLITGPIRRSEAERSLDELAGLADAAGA
                     AVVLRVLQERERPDAATFLGRGKLEALAAACDETGATLAVFDNELTPAQLRQIEKQLQ
                     VKVVDRTQLILDIFAARARTREGKLQVELAQLRYLMPRLAGGSEWLSRLGGGIGTRGP
                     GETKLEVDRRRIRQRIAALGRQIEEVRRRRLQLRERRHKAEVPTVALVGYTNAGKTTL
                     FNRLAGESAVVSNALFVTLDPLVRRVRLPDRRELLVSDTVGFIDRLPTTLVAAFRATL
                     EEVAGADLLLHVIDAAAPGRDRRVAAVRHTLEEVGASEVPTLDVFNKCDLLETVEQER
                     LLRQEPDALCISAATGQGREELLEAITARLAVDVRRLVARLDPASESDRRRLADIYRF
                     GRVHQHVEHDGRVMVEADVPGRLWARLTAGGPADA"
     gene            20438..>21211
                     /locus_tag="PKK95_02050"
     CDS             20438..>21211
                     /locus_tag="PKK95_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC17016.1"
                     /translation="MRDRRAWHKREGVRAAALLACLAAAGGACAPRLAPAPTVTTPAH
                     PDYVYPEVPPVFTRPELTLRLDRGWAFLQTGDPEAARREFSAALAANPGFHPAEAGIA
                     YARLAEGDADAAASAFDRALARDPAYVPALVGRGDALLALDRPDEAAEAYRAALEARP
                     ELPDVRRRLETISFRSQQAALQTARETAASGRYAESVAAYGRAIASSPESGFLYRELA
                     VVERRQGERARALEHLRRAVELDPGDRQSWLLIGELLAEG"
BASE COUNT         3217 a         7421 c         7436 g         3137 t
ORIGIN      
        1 cgcccggtcc ttcttgaacg gcccccccat ctggacggtg tagtcggtga gcttcctcag
       61 gacgtcgccg ccctcgccga ggctcgggtt ctggtcgatg atctcgtccg tcacgttggt
      121 ggcggcccag gcacccctcg tgtaccgcat cgagaagaga ccggagaacg tgttgccgcc
      181 cgacttcgtg atggtgttga ccacggcccc gctgaagccc ccgtactcgg ccggaacgcc
      241 aaggccgccg atctggatgt cctcgatcag gttctggttg aagaaggtcc aggccgaccc
      301 cccctccgga tcgcgcgtgt cgacgccgtc gagcaggagg gcgttgccgt agtagtcctg
      361 cccgccgaag gccgactcgt cgttgatccc gggcgcggcg ttcatcaggc ccgtggccgt
      421 cgagtcgtag agcgcgatcg aggacagcag gtcgccgctc acgttggtcg tggtcgccga
      481 gctcttcacg tccaccgtgg acgcggtgcc cgtcacctcg accgtctcgg tcagcccgcc
      541 gaccttcagc gagaagtcga gcgtcagcgt tcggcccatc gtgacgatga ccctgtcctc
      601 ctgctggggg gtgaagccgg caagttcggc cttcaacgtg taggtggccg gctgcacgcc
      661 cacgaaccgg tagttcccct cggcatcgct caccgcggtc tggacccctc gcggccctgt
      721 gagcgtcacg gtgacgccgg gaagcacggc gccctgctgg tccatgacgc ggcccagcac
      781 ggtgcccgtc tgcgtctgcg ccgaggccgc tcccgcgaat gcgagcgcca gcagcaggag
      841 cgcggtgacg aggcatgaag ctcgtttcat ggtcgcgcct cctacttctt cgccggcgag
      901 gagatgcggt cgttctgcgt cgagtacacc gcgtacccga cgccggccgc aaagacgatg
      961 agcgccgcga tgccgaccgg cttcttgaag aacgacaccg accccgcatc ggagggaccg
     1021 ctttgtgccg ccggagcggc tgtcgccgcg agcggctcgc tcgtcgcgat ggccgccgcc
     1081 gagaccctga tgcccggctt caccgccgct ggcccctcgt cggccaggac ggggaggggc
     1141 aggagcgcga ccgccagtgc gagcgcgaag atccgcccgc aatggcgccg ccggagaaga
     1201 accgacatgg atcctcctct ccgcgatgga cgtgtgcgcg cgacgaaccg attgggtgtg
     1261 ggaccgggag aacaggcaca ttctgcgcct gcatcctcca ggtaatcaag agtccaattt
     1321 tcctgcggtg cccggatggg ctgaggaagt gagccggtct agcgtcccag cttctcgccg
     1381 agcgccgcct cgcggacggc atcggcgtgg cggccgacgc ggttgtacag gtcggcgagc
     1441 acgtagtgac cgagctgggc cacgtcccgg cggggttgca gggccaggcc ctcgcgggcc
     1501 aggtcgattg cctcctggaa gcggacgcca tcctcgagga atgccttcgc caggaagatg
     1561 cgaccttcgg caaaccgcgc gttcatctgc acgctctgct cgagcgctgc caccatgtcg
     1621 gcgcgctgac cgagccgctc gtgcagccgc ccgaggttga acgcggcgcg gtaggcgttc
     1681 ttgtgcaact cgatctcccg ccggtactcc gcgagggccg tctgccagtc ctgccgctcc
     1741 tcggcgatca gggccaggtt gtagtgggcg agccggaccg tggggcggat ggcgagcgcc
     1801 gcgcgcgtct cgcgctcggc ggcggcgagg tcgccccgct ggaaggcgat cacccccagg
     1861 gcgttgcggg ccgaggcctc gcgcggatcg atctcgagcg ccttccggaa gttctcctcg
     1921 gcttgcgcca cgtcgccccg atcgaggtag atctcgccca tctggtagcg gacgggagcg
     1981 ttcttcgggt cgatggtcag gtagtgctcg tagccggcca gcgcggcctc gtcctgcccc
     2041 agctgccggt acgcgttggc catgttgatg accggcaggt cgtaatccgg cttcagctcg
     2101 agggtcctct tgaagtagcc gatcgcgtcc tcgtagcgcc gctgccggaa atggacgttc
     2161 cccaggttga accaggcgtc cacgacgttc ggatcctcgt cgagcacctt gcggtagagg
     2221 gcgaccacct tctcgaagcc gccctcctgc tgcgccaggt cgcgcgcctc gcccatcagg
     2281 ttgaacacgc cgatcttgtc cttggggtcg gcccgctcgc tgcgcgggcc cgcgagggtc
     2341 gcgacgaacg acccgacgta ccccagcgcc gccaggcgtg cgcgcgcctc gggatccacc
     2401 tcgggggccg gacgggcaac cgtctcgtca gactcgatct ccggctgccg ctcgcggagc
     2461 cgccggatca tgccgtcggc gagcgcctgc cgctcggcgt acaggttggc cgtctcgttc
     2521 gggtcgcgct ccaggtcgta cagctccggc cggggggcgt cgatcacctt gtaccgcccc
     2581 gagcgccacg cgcgcaggtc gctccacccg taatggtgaa gggggtagag cgcctcggcg
     2641 tagccctcga ggtcgagcgt cggccggtcg ccggtcatca acggggcgag gctgaccccg
     2701 tcgatcccct cgggcgccgg gatcccgagg aggtcgagcg ccgtcggcat caggtccacg
     2761 gtccgcaccg ggtcggccac gcgccggccg cgcgtccgct cgaacggcgc ccggacgatg
     2821 aacggcaccc gcgtggtgct ttcgtagatg aagaagccgt gagttccctc gtcgtgctgg
     2881 ttcagccctt ccccgtgatc gcccaccacg acgatgacgg tacggtcgag cagggcatcg
     2941 cgctcgagga actcgacgac ccgtgccacc tgcgcgtcgg tgtaggcgat ctcggcatcg
     3001 tacgggtgcc ccgggaagcg cgaggcgaac ggttcgggcg cgtcataggg cgtgtgcggg
     3061 tcgtagaagt gcagccaggc gaagaaccgc tcgcccttga cctgctcgag ccacgggagc
     3121 gccttgtcca ccacgtcggc gccccgccgc tggatctccc cgacactgaa gccccggccc
     3181 gcgggccgat ccagctcgaa atcgtcgccg tagaagtcga agccctggtc cagcccccac
     3241 ttcccgtcca gcacgaaggc gccgaccacc gctcccgtcc gcagcccccg cgccttcagc
     3301 tcggtcgcca gggtccgctg ccggggcgag aggaagaacc ccccgttgtc gcgcacgccg
     3361 tgccgcggcg ggtgccgcga cgtgaagatc gagcagtgtg ccgggagcgt gagcggcgcg
     3421 tcggtcatcg cctgctcgaa gaccacgccc tcgcgcgcca accggtcgat cgccggcgtt
     3481 cgggcaccgg cgtaaccgta ggccccgatc cggtccgctc gcgtcgtgtc gagcgtgatg
     3541 accagcaggt tcagctccga cgcccgcccc cccccggtcg cagctccgag cgcgacgcct
     3601 gcagcagttt cttgcgggcg ccgtcctgcc caccaccacg cggcgccggc tgccgccagc
     3661 gccatcacgg cgacgaccgc ccccgcgcga agccagagtc tcggtgaact ggaagggttg
     3721 gccggcggag cagggcgcgc gtcttctggt ctgggcatcg ggaatccgga gcgtgctggt
     3781 tcatgctcca ccgagtgcca gcgggccgtc aacccatccc ggtcgccacc ttcaatctcc
     3841 ggacagttgg acacttcatc cgacaatttg gatcgtgacc gggtgtcgct ggcgtcggaa
     3901 gcgccgtcgg cctcgccggc tcaacaactt gcgggcgcat gcctcgcccg cggccacggc
     3961 agggcttttg attgggcagg gccaatcccg tcccggtcgg tcatcggtcc ggcctcgtcc
     4021 cctgcccccg tgaaggagga agcgaaggtg agagaactcg ttcgagcctg gcgacccgcc
     4081 gtgcgactgc ttgcactcgc gcttgcggtc accgtgctcc ccttgcctgt gtcggcaggg
     4141 caacaggacc agacgactcc gaaaccgggg atccgcgcgt cgatcgccaa ggcggttgcg
     4201 gtggagcctc cggcggcgtc gaaggatgca gtagccgtgg tgcaggacga ccccggcgcc
     4261 aagctgaact cgccgtcgtt cttcaagact ccagcgggag ttgccgtgat cgccatcatc
     4321 gcggcgggga cggggttcgc catctactcg gcgagcaacg accgcatcaa gtcaccgggc
     4381 cgctagggga ggcatggaca tcatgaaaag aatcctgatg ggcctggccc tgatcgcggc
     4441 gttgatggct tgtgccgacg cggcggttgc gcagtcgctc accggcacga tcgccggccg
     4501 ggtggccgac gaacagggcg gcgtgctgcc gggcgtcacc gtgacgctga cgggtcggac
     4561 cgggtctcag acccaggtca ccgacgagcg cggcgagttc cgcttcatcg cgctcgagcc
     4621 cggcatctac tcggtaaagg ccgagctcga gggcttccgg gcgaaggaag aggcgaacat
     4681 ccaggtcggc atcgggcgca ccgccgacgt gcgcctcgcg ctgggcgtcg gcggcctctc
     4741 cgagaccgtc gaggtcacgg ccaacgcggt gacgatcgac acgacgacga ccgccaccga
     4801 caacacgatc tcgaaggacc tgctgttcgc catgccgctc acgcaggcga acccgtcggt
     4861 caacctgctc gactacacgc cgggcgtcaa cgacaactcg gcgttcggcg gcacgggcag
     4921 ctacggcaac gccctcctgc tcgacggcgt ggacacgcgc gacccggcgg gcggcagttc
     4981 ctggacgttc tacggtttca acatgatcga ggaggtccag gtggccggcc tcggccagcc
     5041 ggccgagtac ggcggcttca ccggcgcggt ggtcaacacg atcaccaagt cgggcggcaa
     5101 ccggttctcc agcctgctgg agtaccgcta caccaacaag gacctggcgg gtgacaacac
     5161 cagcgacgcg atcgcggcca tcaaccccgg gctcgcgacc ccctcgcgct gggacaagat
     5221 gaacgactac accgtccagc tgggcggccc gctgaagaag gaccggctgt tcttcttcgg
     5281 cagcgtgcag cggtactcca tcaaggaaga cccggacggc ccccgcacgg ttcgcacgga
     5341 ggtcagcccg cgcttcaact tcaagctgac ggcgaacctc acgtcgagcg acacgctgag
     5401 cttcggtgga cagtacgact ggtacaacca gaccgggcgc gtggcgtggg gcccgaccgc
     5461 ggctgccacc gacgaccgca cctccgacca ggactcgccc gaggcggtct ggaacgcgca
     5521 gtaccgcaag gtcttcggct cgtccacctt cctcgaggcg aagttcaccg ggtggtgggg
     5581 ttactactac gtcgatccga tcaagccggg agcggcgcac tacgacgtcg acagcggcgc
     5641 gtactcgggc ggcggcggtg agtacttctg ggccgaccgc ggccgcaacc aggtgaacgt
     5701 ctcgctctcg aagttcgtgc agaaggccgg gcagcacaac ttcaagttcg gcgtcgagat
     5761 cgagcgcagc cgcgtgcgcg accgctacgc gtatgccgag gagcacggcc gcccgatcgc
     5821 ctactacgac tacgccggcg agccctacat ggcgtactcc tactcgtacg acctccaggg
     5881 gcgcaaccag cgcgagtcgt tcttcgcgca ggaccagtgg aagatcggga acgtgacggc
     5941 caacctcggc gtccgcctcg acaacatccg cggccgcagc ccggtgctcg acgagacgct
     6001 ctacgacacc aagtcgctgg cgccgcgcgt cggcctggcc tgggacgtca ccggcaaggg
     6061 cgagtcggtg ctgcgcgcgt tctacggcca cctttacgag gggccgcagt actacacctt
     6121 ctcgtacgcg ctgcccggga tcggcgactt cgtcggctac gaagtgttgc cgggctggca
     6181 gttgggcgag gagatcttca gggtgcctgg cacgagcaag tactcgatgg gcaaggacct
     6241 gaagcacccg cgggtggacg agttcaacgt ctcgtaccag cagcagttcc tgcggaacta
     6301 caagttcacg gcgacctacg tccgccgcga caccaagaac gtcatcgact cggtgttccc
     6361 ggaagcccgg tgggagccct tctcgtacac gaacgcgaag gggcagcaga tgacgctgta
     6421 ccgctgggcc aacgacgaga tcgaccagaa gttcgaggtc cgcaacgtgg acggcttcca
     6481 gttcctgtcg ccgagcgggc aggtgctggg caccatcgac gccttccgca agtacgacgg
     6541 catgatgttc gtcctccagc gcgcgctccg caaccgctgg caggcgcagg tgtcgtacgt
     6601 ctactcgcgc accaagggca cggttgacaa cgacggcagc gacggcctgg gcggcaccaa
     6661 cttccagaac ccgaacacca tcctcgtcaa ccgcaacggc tacgtgtcga acgaccgcac
     6721 ccacgaggtg aaggtgttcg ccggctacca gatcccggtc atcgaggtcg gggtcagcgg
     6781 gtacctgtcg gtcatcagcg gctacccgta ccagccgtac gagcgcctca ccggcagccg
     6841 gatcaactgg accggcgcca agaacgtctt catcgagtcg cgcggcagct accgcaccga
     6901 cgcgatcacc gccttcgact tccgtgccga gaaggtgttc gagttcgcgc cgtaccggtt
     6961 cggcctgtac atggacgcga ggaacctgtt caaccagggg acggtcacct gggtccagac
     7021 gcggtacccc agcgccgaga tctcgggcgc ggccgtgccg ttcggcggcc cggtgtacgt
     7081 gcagagcggc cgccagatca ccttcggcgg acgcttctcg ttctaaaggc ccaggagggg
     7141 gcagggcttc ggccctgcct gaacgtcggc cccgggggct gcggctcccg gggccttttt
     7201 tctgtacact ggtccggctc atggctcgag gctcaggcct cgaggcggag ggctgaaggc
     7261 ctcagggact tgcggcgctg tcgccgcaag cgtagcgagc atgaagacgt ggacgatcgg
     7321 cgaggcggac ggcggggcga ggctcgacgc gtggctggcc cgccagcccg agatcggctc
     7381 gcgagggcgt gcgcggtcgg ccatcgagcg cggcaaggtg ttcctcaacg gagagggcca
     7441 ggacttcgcc gacgcggcaa cccggctgtc gccgggcgac caggtcgggt actgggagga
     7501 ccgccccggc tcggcccgca cccccgctcg cgacgtcgtg tccgcgcgtg ccgcccttcg
     7561 catcgttcac gaagacgccg ccatcctcgc cgtggacaag cagcccgggt ggctcgtcga
     7621 gccgctgccg ggcgaggagg ggagcgaggt caccctgctc gacctcctgc gcgaccacct
     7681 ccgcgccgcc aggcgccgcc ccttcgtcgt ccaccgcatc gaccgcgaca ccagcggcct
     7741 cgtgctgttc gccctgacgc cggcggcccg cgacgacctc aagcggcagt tcgagcgacg
     7801 cacaccagag cggatctacc tcgcggtggt ccacggcctg gtcgtgcccg cctcgggaac
     7861 ctggcgcgat cacctcgtat gggacacgga gcgcttgatc cagcgccgcg ctcacatcca
     7921 cgaggagcgc gcccgcgagg cggtggctcg ataccgcgtc ctcgagcagc acgagggcgg
     7981 ggcggtggtg gaagtgtcgc tggtgacggg caaacgcaac cagattcgtg tccaggccgg
     8041 cctgcggggc taccctctgg taggggagcg gctctacacc tacggccgcc aaggggaacc
     8101 tggcccgcgc ctcgagctgg gccgccaggc cctgcacgcc gccacccttg ccttccgcca
     8161 tccggaggac caccgccccg tccagctcga cgctccgctc ccggacgacc tgcggaatct
     8221 gatcgatcgg ctgcgggcag ggagctgagg ggcctccgtc acccgccttc gccaaacgct
     8281 cgctactctc ggctacgggg ggcaggcctc cagcacctca tgtgaggtac actgtgccgt
     8341 gccttctcga cagatcctga tgatcgcctc cgaggcggtc cctttcgcca agaccggcgg
     8401 ccttggcgac gtcgccgggg cgctgccggc cgcgctggcc aggcagggct gcagcgtcac
     8461 gctgatcgtg ccccgctacc gcgccgtcgc gggcggaacg accatcacgc ggttcccggt
     8521 caccgtcggc ggccgcacgc tcgacgtggg attcgtggag cacccgctgg ccggtgccgc
     8581 gcgcgccgtg ctggtggact gccccgagct gttcgatcgg cccgagctgt acggcgtggg
     8641 gaacgccgac taccccgaca acgccttccg cttcggcgcc ttcacccggg cggccctcga
     8701 gttcgccgcc cgcctggacc tgcaccccga catcgtccac gcgcatgact ggcagtcggg
     8761 actggcaccc gtctacatcc gcacgcttta cgcgaaccat cccgagctgg cccgggccgg
     8821 gctcgtgttc acgattcaca acctggcgta ccaggggttg ttcccgccac actggctgcc
     8881 cgcgctcgac ctgggctggg acctgttcgg catcgacggc ctggagttct ggggcaaggt
     8941 cagcttcctc aaagccggcg tgaacttcag cgacgtcatc acgacggtca gcgcgcggta
     9001 cgcgaaggag atccagacgc ccgagtacgg gttcgggttc gacgggattc tccggcgccg
     9061 cgccgaccgc ctcgtcggca tcctcaacgg gatcgacacc gccgtgtggg acccggcctc
     9121 cgatccccac ctgcccgagc cgtacgacgc ggaccggctc gagcgcaagc ggtcgtcgaa
     9181 gcgcgcgctg ctcgaggcgg tcgggctgcc atccgacgac caggcgctgg cgcgcccgct
     9241 ggtcgggatg gtgtcgcggc tggtggacca gaaggggttc gacctgctcg cgctgctggg
     9301 cgaacgtctg ctggccctcg atgcctcctt cgtgctgctc ggcagcggcg agccccagta
     9361 ccaggaacag tggcagggcc ttgccgcccg ggcgccctcc cgcgtggcgg tccgcatcgg
     9421 attcaacgag ccgctggcgc acctgatcga ggcgggcgcc gacatcttcc tgatgccctc
     9481 gcgcttcgag ccgtgcggct tgaaccagat gtacagcctg aggtacggga cggtcccggt
     9541 ggtgcgggcc accggcggcc tggatgatac gatcgtgaac tggagcccgc gcacgggccg
     9601 cgggacgggc ttcaagttcg ccgatgccac gcccgaagcg cttctcgcgg ccttgcggaa
     9661 ggcgctcgag gtgtaccgcg acccctccgc ctggcgctcc ctccaactgc agggcatggc
     9721 ctgcgactac tcgtgggacg cctcggcccg gcattacgga acggtgtacg agaacgcgat
     9781 ccgcgccagg gccaagcggg cgtaacctgg ggcgcgggcg gcgaatcctt gaacaagaac
     9841 ggggcaacgc cccgccgtcg aatcttcagc gaaggagcac tcatccaatg gcagcagccg
     9901 cgatccagca ggtgaccgac agcacgttcg agcagctcgt caagtcgtcg acccccaccc
     9961 tcatcgattt ctgggccgaa tggtgcatgc cctgccgccg tgtcgccccg gtcgtggacg
    10021 cgctggccac cgagtacgcc gggcggctca acgtcgccaa gatgaacgtg gacgagaacc
    10081 agaacgtgcc gatccgcctc gggatccaga gcatcccgac gctgatgatc ttcaaggacg
    10141 gcaacctcgt cgatatgcac gtcggcgcca ccgacaagga cgtcctcagg aagatggtgg
    10201 acaaacacgt taagtgacct gtcggttacc tgagccatga acgaagtgcg agacgtcatc
    10261 atcatcggat cggggccggc cgggctcacc gcggcggtgt atgccgcgcg cgcgaacctc
    10321 cgcccccttc tcatcgaggg ggccgaggcc ggcgggcagc tcatgctcac aacgaccgtc
    10381 gagaactacc cggggtttgc cgacggcatc ctcgggcccg agctgatcgg ggcgatgcgg
    10441 agccaggccg agcggtttgg taccgagttc gtccaggcca acgtcgaatc ggtggagctg
    10501 tcgagtcccc cctacgtcgt gaagaccgcc gacgaggaat accgctgccg gaccctcatc
    10561 gtggcaaccg gtgcttcggc gcgcctgctc ggcctgccgt ccgagcggca cctgatgggg
    10621 cacggggtct cgacctgcgc cacgtgcgac ggtttcttct tccggggcaa accgatcgcg
    10681 gtggtgggag gcggcgactc ggcgctggag gaggcgaact tcctgacgcg gttcgcctcg
    10741 aaggtgaccg tgattcaccg gcgcaacgcc ttgcgggcgt cgaagatcat gcaggagaag
    10801 gcgttcgaga accccaagat cgaattccgg tggaactccg tcgtcgagga ggtgaaggac
    10861 gtcacgcgcg ggcaggtcac cagcctcgtc ctgcgcgacg tgctcaccgg cgagttgagc
    10921 gagcttcgcg tggacgggtt gttcgtcgcg atcggccaca cgccgaacac gtcgctgttc
    10981 gccggccagc tcaagatgga cgccaacggg tacttgatca cccacgacgg gacgctgacc
    11041 aacgtgccgg gagtgttcgc ctgcggcgac gtccaggatc acgtctaccg ccaggccatc
    11101 acggccgccg gatcgggctg caaggccgcg ctcgacgccg agcgttacct cgagcacgtc
    11161 atggtcgaag gcctggggcg agaggtcggc gacaccctga agcctgcgta gggtgcagga
    11221 tcattcaatc cagcccccac ccacgacctc gtccccatcg tagaaaaccg ccgcctggcc
    11281 cggcgtgatc gcggcctggg gctcgtggaa gtcggcacga gccccagcgt cgccctccgg
    11341 gctcaccagg gccggtgcgg cctggtgccg tgagcggatc tgcacgtgca gcgtcgcggg
    11401 cgatgccggc tggcgccccg acacccagtt cacgcgcgcg gccgacagcg agcggcgctc
    11461 gagcgcctcg cgtggcccga ccatcacctg gcccgtcccc gacgacaccc cgatcacgta
    11521 caacgggaca cccactgaca ggttgaggcc cttccgctgc ccgacggtga agcggtggat
    11581 gccgccgtgg cggcccagga cgcgcccctc gcggtcgacg atctcccccc cggtcacgcc
    11641 gggtgccgca cgctcgacga ccgccgcgta gtcgccgccg gggacgaagc agatctcctg
    11701 gctgtcgcgc ttgtcggcga ctgcgaggcc aagcgaccgc gcgatgcggc ggacctccgt
    11761 cttctcgagc ccccccagcg ggaacatggc gcgggcgagt tgcgcctggg tcagcccgaa
    11821 caggaagtac gactggtcct tcgaggcgtc caccccgcgc aggaggcggt acctccccgc
    11881 gtcgtcgtcc cgctcgatgc gcgcgtagtg gccggtcgca accgcctcgg cgccgaagag
    11941 atcagcccgg ccgaccagct cggcgaactt gagatcggtg ttgcagtgcg cgcagggcag
    12001 gggagtgcgt cctgccaggt actccgcgac gaagttgtcg atgaccgtct cctggaaccg
    12061 gcgctgcagg ttgaggacgt aatgggggat cccaattgcg gcggcgacac ggcgggcgtc
    12121 gtgcaggtcg tcgagcgagc agcacgagcc gaagccggac gccccctcgc gctggtcgta
    12181 cacctgcatc gacacgccga tgacctcgta gccgcgctcg acgaggagcg ccgccgtcac
    12241 cgacgaatcc acgccgccgg acatggcggc gacaactctg gtcatggtca ggctcaaggc
    12301 tcaggttcaa agctcagggc tcaggctcag ggaatcgcga cggctcaggc tcgagggctc
    12361 aggccctaca acacgcgccg gattcgctcg acgacccccg ggagcaccgc gagcacgcgg
    12421 tcgatgtcct cgtcggtggt cgccggcccg aggctgaacc ggatggcgct ctgtacacgg
    12481 gccggcggaa ggcccatcgc ccgcaggacg tgggacggct ccagggtgcc cgacgagcag
    12541 gccgaccccg acgacacggc aatcccttcc aggtcgagcg cgatcagcag cgactcggcc
    12601 tccgcgcccg ggaagctgat gttcgtcgtg ttcggcacac gcccggcgcc ggcgccgttg
    12661 accacgctgc cgggcaccgc cgccagcacg gctcgctcga ggcggtcccg gcgcccggcc
    12721 acggccgcgc gcttgttccg gcttccggca gcgagctccg ccgccacgcc cagcccgacg
    12781 atcccaggga cgttctcggt ccccggtctc cggttgcgct cctggcgccc cccggtgagg
    12841 acgccggcca tcggcacccc tcgccggatc cacagcgccc ccgcgccttt cggccccccg
    12901 aacttgtggc cggtcagcga cagcaggtcg acgtccagct cgcgcacgtc gacctcaatc
    12961 ttccctatcg cctgcacggc gtcggtgtgg aagcgcgcgc cggcggcgtg cgcgagcgac
    13021 gcgagggcgg caaccggctg cactacgccg gtctcgttgt tcgcgagcat caccgaaacc
    13081 agcgcgacgt cgggcccgag gtgcggagcc agcgcctctg gggtgaccac tccgtcgctc
    13141 gtcacgggca cgatggtcac cggccagccg cggcgctgca gcgccagcgc ggtgttgagg
    13201 accgcctcgt gctccaccgc ccccacgacg agcctccggc gggtcgccgg cgccgcttcg
    13261 agcgcgccgc gaaccgccag gttgtccgac tcggttcccc ccgaggtgaa gacgatgcca
    13321 gccgggtcgg ccccaaccag cgcggccacc gcctgccgcg cctcgtcgag cgcacggcgc
    13381 gcccgctgcc cgggcgcgtg aaggctggag gcgttgccga aggtgtcgtg caaggccgaa
    13441 gcaacggctt cggcaacctc cggggcgacc ggggtggtgg cgttgtggtc gaggtagacg
    13501 ggcaccatgg gacagcacgc cgcgctttct cgcccgaatg cgcgtggcgc ggcctgactc
    13561 cattatccgt ggggggctgg gggtggacaa ggttcgcgcc gatacatata ctggcgaggc
    13621 ttttctcggc cacctcagca caggtttggc tgtcaccccg ggtgcccgtc cccgctgccg
    13681 ccagcagtgt gggtgttggg aggaatgaag caatgtggaa gcgtgatgag agcgtgcggc
    13741 cgccgtccgg ccgcgacgct gtgggcgtgc aaccgcccgt cccgcaggca ggtgtccccg
    13801 gcgcacccgc gcagggggcg gacatcgagg gcagacgcca catggaaacg agagacgtcg
    13861 tgaacattgg gaaatccgtc gtcatcaagg gcgagctcaa cggaagcgag gacctgaccg
    13921 tcgaggggca cgtcgaaggc cggatcgagc tgaaggatca cgcgctgacc atcggcccgc
    13981 acgggcgcat caaggccgag atccacgcca agtccgtgat catcctcggc gaggtgacgg
    14041 ggaacatcat cgcgagcgag aaggtggaca tccgcgacaa cggctcggtg gacggcgaca
    14101 tcgtcgcccc gcgggtcgcg attgccgagg gggcgcactt ccggggcagc gtggacatgc
    14161 agcgcaagag cgcgcccact ccgccgccgg cgcccaaggc cgaggccaag ccggcgccgc
    14221 ccgccgcgcc gggtcagccc gccaaggccg gcgtctagcg agacgcagcc ggccgcccct
    14281 gccggggcga cctgctagac ttggtccaca caggcagccc ggttggccgg ccggcacacg
    14341 gccggcggcc gggcctgccc gctccacgat gagaggcaat cccgtgtcgg gtggtggaaa
    14401 gtgacgggtt tcctgtcgcg attcggccag cgccgcgggg ccgaccccga cgacgtgccg
    14461 gagaccgccg gcgccccgga cacggtggtt ggcaccaagg cgctccgcaa gttcatctcg
    14521 tacctgaagt cgcgtccgac gccggtgctg ctcgacctgg gcccggtcgt cggttcgaac
    14581 atcagcttct tcggcgagca gatcggctgc aagatcttcg tcgaggacct gttcgccgac
    14641 atcgaccgcc acgagcgcga gggccggctt gccgacctgc cggcgttcct gcgctcgcgc
    14701 ttcccgcagg cctccggcag cgtggacggg gtgctgtgct gggatctctt cgacttcctg
    14761 gatcgctcgg cggcccaggc ggccgccgag cagctgtcgc gggtgctggc ggtcaacggc
    14821 gcgctcttcg gcttcttcgg catgacgcgc ccttccgagg tgcgcctgac gaagttcgtc
    14881 gtcgtcgatg acgcgaacct gcggcaccgg ccctattcca tcccccgcat gcgccaggcc
    14941 gtgctgcaga accgcgacat cacgcgcctg ttcgagccgc tccgggtctc agactccttc
    15001 ctcctcaaga acaacgtccg cgagatcctg ttccgcaagc cggattacat ggacagccga
    15061 tagcgcgccg accgaggagg gctcgctgca cacgcgctgc cacggcccgg catccaaggg
    15121 tagaataccg gtgggcaact catcgggccg ccggcctcgc cggcgagccc ttggaaggcg
    15181 gccatgcgtc caatcgtggc gctcctcacc gatttcggcg tccgcgatca ctacgcgggc
    15241 acgatgaagg gcgtgatcct ggggatctgc ccggaagcga ccctcgtcga catctcgcac
    15301 gacgtcccgc cacacgacct gcggaccgcg tcgctcgaac tggcggccag ctacaagtac
    15361 tttcccccgg gcaccgtctt cctggtcgtc gtcgaccccg gcgtcgggtc ggggcgcagg
    15421 ccgctggccg ccgaggcggg cgagtaccgg ttcgtcgcgc ccgacaacgg cgtgctgaca
    15481 gcggtgctca aggagacgcc gccacgccgc gtggtggagc tgaccgagcg ccgctacggc
    15541 cgcccgaccg tcagccgcac cttcgaagga cgcgaccgct tcgccccggc agcggcctgg
    15601 ctggccaagg gtgtcgaggt cagggcactt ggccgcccgc tcgccgacta cacgctgctc
    15661 gacatcccgg acgcggaggt cggcaccgac gcgattgtcg gcgctgtgct gcgcgtcgac
    15721 cggttcggca acctgatcac caacatcgac cggcgctcgt tcgaccgcct ggcgcggctg
    15781 ggcggccgcc tgcacatccg cgtgggcgag gcggacgtgg cgcagctggt gagcacctac
    15841 gcggaagtgc cggccgggga agtggccggg ctgttcggca gcagtgatca cctcgagctg
    15901 gcggccaaca gcgccagcgc ggccggcctg ctcggcgcgg ggcccgacgc ggtggtcacc
    15961 atcacgcgca tcgccgagga acaggtttga gatggacttc gacctggggg aagaacacct
    16021 gttgctggag cagaccgtcc gcgagtgggc cgagcgggcg gtggcaccgc gcatccgcga
    16081 gctggaccgc gaacaccgct tcgatgcgtc gatcctcgcg cagatggccg acctggggct
    16141 gctcggcgtt tctgtgccgt cgtcctacgg cggcaccggg accgactaca tcagcctcgg
    16201 ggtggccagc gaggaactgg agtacgtgga cacgtcgctg cgcgtgatcc tgtcggtaca
    16261 cgtcggcctc aacagcctga ccctgctggc gtggggcacc gaggcgcaga agcagcgcta
    16321 cctggtgccg caagcccagg gaaagaagat tgccaccttc ggcctgaccg agccttccgc
    16381 cggcagcgac gtgcgcggca tccagagcgt ggcggtcaag cacggcgacc gctaccggct
    16441 cacgggtgag aagcagtgga tctccctggc tgacgtcgcc gaccacttcc tcgtcttcgc
    16501 ctggaccgat ctcgagaaga agaagcagcg cgacccgtcc gggctgacgg ctttcatcgt
    16561 cgagcggatc ttcccgggct tctcgagcgg gacgctgaag gagaagtggg gcatcctcgc
    16621 cggcaacacc ggcttcttcc ggatggagga ggtcgaggta cccgaggaga acgtggtcgg
    16681 ccggccgggc gaagggttca agatcgcgat gttcgccctc gaccagggcc ggtacacggt
    16741 ggcggcggga gcgacggggc tgattcgggc gtgccgcgac gcctcggtga agtacgcgaa
    16801 ggagcgccgc gccttcggcg tggaaatcgc ccggcaccag ctggtgaagg cgatgatcgc
    16861 gggcatggag tccgactacc aggcggcccg cctcttgtgg ctccgcgcgg gctggctgaa
    16921 gaacctcggg cgccggaaca cccgcgagac gagccttgcc aagtggttcg ccaccgtcgc
    16981 ctccgaacgc gcggccggca acgcggtgca ggtgcacggc gcgaacggct actccgacga
    17041 ctacccggtc gggcgcttct accgcaactg caagggcgcg gtgatctacg aggggacgcg
    17101 cgagatccac cagctgatgc aggctgatta cctgctcggg taccgcgagg accgcaccac
    17161 gaggtgcacg ctgcccccgg ttgggagcgg ggacacctaa tgtagaagca gaatgtagaa
    17221 agatcgatga gaccactccg gcctcccttt ctacattctt catttttcac tctacatttc
    17281 gaatcgaagg gggagccccg tggtcaccaa cctctcgctc gagttccttc gcgtcgtcga
    17341 gcgcgccgcc atcgcgtgcg cgcacaccat gggccagggg gacggcccgc gcgccgaccg
    17401 tgcggccgtc gaggcgatgc gacgcgagat ggacgtgctg ccgatcgacg gcacggtggt
    17461 gatcggcgag ggcgagcgcg acgaggcgcc gatgctctac atcggcgagc gggttggaca
    17521 ggcgcacctg cggggggcag ccggcggagc gttcgacaag gtggacatcg cggtggatcc
    17581 cctggaaggc accaacctct gcgcagtcgg tatgcccggg gctatctccg tgctggccgc
    17641 ctcggggccg ggcggcctgc tccacgcgcc cgacctttac atggagaagc tgatcgtcgg
    17701 gccgaagtcg aagcaggcgg tcgatctcga cgcgccggtg gccgacaacc tctcggcgat
    17761 cgcgcggtgc ctggagcgcg aggtgggcga cctggtcgta gtggtgatgg accgtccgcg
    17821 ccacgagcgc ctgatcgccg acatccgccg cgcgggggcc cgcatccgcc tcatcaccga
    17881 cggggatttg tcggccggca tcgcggccgc cgtggtgggc agcggcgtgc acgcggtgat
    17941 ggggattggc ggggcaccgg agggggtgct cacggccgcg gcgatgcgct gcctgaacgg
    18001 cgaaatcttc ggccgcctgg tcatccgcac ggccgaggac gagcggcgct gccgcgagat
    18061 ggggatcgag gacgtgcgcc gggtctaccg cgcggccgac ctggcgccgg gcgggagcgt
    18121 catcttcgcc gctaccggcg tgaccgacgg cacgctgatg cgcggcgtcc gtttcttcgg
    18181 tgacggcatc cggaccagct cgctggtgat gcagaccgac ccgcacctga tccgcttcat
    18241 cgacagcatc tacgtgcagt acgggcagga cgtccgcatc cgcttctgac ccctatccct
    18301 cgtcttgtcc cttgtgcttg cgcttgcgcg cggggcgctt gcggcggtct tcgtgcgcgc
    18361 cgccgacctg gcggggcagg ggaacgcggg gcagcttcgg cttccgggtc ctgggtgctc
    18421 gcttcatgtc tgccgaacca gcgatggcct ctcccgggtc gaggccgaac ggacacgatt
    18481 gaagcccttg gtccgccggt cacgcctcgg ccgtggatgc gataatagca gcgtgcgcac
    18541 gtggacggtg aacccctcgc gtcccgacga gtcggtcctc gccgaggcgg cggcggtcgt
    18601 tcggcaaggc ggcgtcgtcg tcttcccgac agacacgctg tacggcctgg ctgccgaccc
    18661 gtgcgacgcg cgcgcggtgg acaaggtgtt cgccgtcaag ggccgcgccg gccgcgagcc
    18721 gctcccgctg atcgtcgcag gcctcgagga ggccgagacg attgcgcgga tgacgacgct
    18781 tgcccggcgt ctcgcggccg tcttctggcc gggtcctctc accctggtgg ttgacgaccg
    18841 cggcatcgtg gcgccggcag cgcacgccgg caccggcacg atcgggatgc gcgtgcccgc
    18901 gagccccgtc gccgcggggc tggcgcgcct ggcgggaggc gtcgtaacct cgacgagcgc
    18961 gaaccgctcg ggcgccccgg cgccggtttc ggccgcccag gcctgggacg ggctggggga
    19021 ggcggtggac gggttgctcg acggcgggcc cgcgccgggt ggccgcccat ccaccatcgt
    19081 ggacgcgcgc ggggagcggc ccgtgatgct gcgggcgggt gcgatcgagt gggagcgggt
    19141 cgtggaggcc ttggaacgtc gatgacggcg aagcggcagc gggaggcggg agagcgtgcg
    19201 gccctggtcg gcctgatcac ggggccgatt cggcggagcg aggcggagcg gtcgctggac
    19261 gaactggcgg gcctggcgga cgcggcgggg gctgccgtgg tcctgcgcgt gctgcaggaa
    19321 cgcgaacgcc cggacgccgc gacgttcctc gggcgcggca agctcgaggc gctggccgcc
    19381 gcctgcgacg agacaggggc cacgctcgcg gtcttcgaca acgagctgac gccggcgcag
    19441 ctgcggcaga tcgagaagca gctgcaggtc aaggtcgtcg atcggacgca gttgatcctc
    19501 gacatcttcg ccgcgcgggc ccgcacgcgg gagggcaagc tgcaggtgga gctggcgcag
    19561 ctccgctacc tgatgccgcg gctggcgggc ggaagcgagt ggctgtcgcg cctgggtgga
    19621 gggatcggca cgcggggacc aggcgagacc aagctcgagg tggatcgccg gcggattcgc
    19681 cagcgcatcg cggcgctcgg ccgccagatc gaggaggtcc ggcggcggcg cctccagttg
    19741 cgggagcgcc gtcacaaggc ggaggtaccg accgttgcac tggtgggcta caccaatgca
    19801 gggaagacca cgctcttcaa ccgcctggcc ggcgagtcgg ccgtggtctc gaacgcgctg
    19861 ttcgtgacgc tcgacccgct ggtccggcgc gtccgcctgc ccgaccgccg ggagctgctc
    19921 gtgtcggaca cggtgggctt catcgaccgc ctgccgacga cgctcgtggc ggcgtttcgt
    19981 gccacgctcg aggaagtcgc gggggccgac ctcctgctgc acgtgatcga cgcggcggcg
    20041 ccggggcgcg accggcgggt cgcggccgtg cgccacacgc tcgaagaggt gggggcctcc
    20101 gaggtgccaa ccctcgacgt gttcaacaag tgcgacctgc tcgagaccgt cgagcaggag
    20161 cggctgctgc ggcaggagcc cgacgcactg tgcatctcgg ccgccaccgg gcaggggcgc
    20221 gaggaactgc tcgaggcgat caccgcgcgg ctggccgtcg atgtgcgccg gctggtggcc
    20281 cgcctcgacc cggccagcga gtcggaccgc cggcggctgg ccgatatcta ccgcttcggg
    20341 cgggtgcacc agcacgtcga gcacgacggc cgcgtgatgg tggaggccga cgttcccggc
    20401 cgcctctggg cgcgcctgac cgccggaggg cctgcggatg cgtgaccgac gcgcctggca
    20461 caagagggag ggtgttcgcg ctgcggcctt gctcgcctgc ctggctgccg ccgggggagc
    20521 gtgcgcgcca aggctggcgc ccgccccaac ggtgaccacg cctgcgcacc ccgactacgt
    20581 ctaccccgag gtgccgcccg tcttcacgcg ccccgagctg acgctcaggc tcgatcgggg
    20641 ctgggcgttc ctgcagaccg gagacccgga ggccgcgcgg cgcgagttct cggccgcgct
    20701 cgcggccaac cccggcttcc acccggccga ggccgggatc gcctacgcgc ggctggccga
    20761 gggggacgcc gatgcagccg cgtcggcgtt cgaccgcgcg ctggcacgcg acccggcgta
    20821 cgttccggcg ctcgtcggga ggggtgacgc gctactcgcg ctcgatcgcc ccgacgaggc
    20881 agccgaggcg taccgcgcgg ccctcgaggc ccgccccgaa cttcccgacg tgcgccgccg
    20941 gctcgagacg atctcgttcc ggagccaaca ggcggcgctg cagacggccc gcgaaacggc
    21001 ggcgtccgga cgctacgcgg agtcggtggc ggcctacggc cgggcgatcg ccagctcgcc
    21061 cgagagcggc tttctatacc gtgagctggc ggtggtcgag cggcggcagg gtgagcgcgc
    21121 ccgcgcgctc gagcacctgc gccgcgcggt cgagctcgac ccgggcgacc ggcagtcgtg
    21181 gctgctcatc ggcgagctgc tggccgaggg g
//
LOCUS       DAOPQX010000019        20837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3151_length_20837_cov_5.241122, whole genome shotgun sequence.
ACCESSION   DAOPQX010000019 DAOPQX010000000
VERSION     DAOPQX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 20837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20837
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_20837_cov_5.241122"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PKK95_02055"
     CDS             <1..584
                     /locus_tag="PKK95_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC17017.1"
                     /translation="IEWRRVPAAPSRPAFEIAATETTVGQFRAFSRATATPLPRQPHW
                     SDEDHPVVNLTWAEASAFCTWAGGRLPTEAEWESAASAGGTGQYGWGDTWDPSRANAA
                     GVAGNDVWGYTSPVASFPPNALGLYDMVGNVWEWTADLYSPEPANRGRRSLRRAVRGG
                     SWANRRENVRLSCRDGLSESGRHNLHVGIRCVR"
     gene            676..1059
                     /locus_tag="PKK95_02060"
     CDS             676..1059
                     /locus_tag="PKK95_02060"
                     /inference="COORDINATES: protein motif:HMM:NF017917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HOC17018.1"
                     /translation="MKKSELPSTLKRSSPKAQRTYAKTHDSAVRSYGEGERAHRTAIS
                     SLKHSFEKVGDRWEPKKKRGPSDPRAAKPASEARAGKGETFGGVDYAGRSKGELYERA
                     RALQIGGRSGMSKEELARAIAKKQR"
     gene            complement(1148..1321)
                     /locus_tag="PKK95_02065"
     CDS             complement(1148..1321)
                     /locus_tag="PKK95_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17019.1"
                     /translation="MVKGRCTRCQIFVVHEDIDRLILSGWKSLEGTQPMVRDIVPMLC
                     PDCRRDEQLRRER"
     gene            complement(1403..2128)
                     /gene="prxU"
                     /locus_tag="PKK95_02070"
     CDS             complement(1403..2128)
                     /gene="prxU"
                     /locus_tag="PKK95_02070"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340894.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1877..1879),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HOC17020.1"
                     /translation="MAEAPKGCARTTGAVVGEPPPEAAGPEPVEPRRAAMMTARVGQK
                     APDFQAPAYYRGSFTNVKLSDLLGKWVLLCFYPGDFTFVUATEVSAVADKYPVLQELG
                     VEVLSVSVDSHFVHKMWNDHELVRMVEGGVPFHMVSDQGGNVGRAYGVYDEQQGIELR
                     GRFIIDPDGVIQAMEVLTPPVGRKFGETVRQIQAYQHVRNSKGTEATPAGWEPGQPTL
                     TPGPDLVGNVWKVWNPSMEKTGV"
     gene            complement(2186..3865)
                     /locus_tag="PKK95_02075"
     CDS             complement(2186..3865)
                     /locus_tag="PKK95_02075"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOC17021.1"
                     /translation="MPFHFRSLVALAAAVACLSAAPPGRARQQPEAYDLLIVNGTLVD
                     GTGRPGIAADLAVSGGRIARIGRLPGVSAKAVIDAKGRSVAPGFIDVHTHADGPVRTP
                     LVENFVRMGVTTLVAGNCGDSAESVASELQAITREGLAVNFSTLVGHNTVRAAVMGAA
                     GRAPTPAELERMKALVAKAMREGAIGLSTGLQYVPGQYAERGEIIELAKVAARAGGLY
                     ASHMRNEGTRVAEAVAETIAIGEAASCPVQVSHLKIDSPNRWGTSQALLDAIASARRR
                     GVDVRADQYAYTAGSAGLGIRFPGWALEGGRPAIEARLRDEATWRRVKDGMKALLAER
                     GFTDLAWAVIALDRDDPSLNGLTVKQAAARLLGKDDLEAQLETARRLMLGSEPQMVYH
                     FMSEDDVKRIMRDPNVMIASDSGVLRPGEGTPHPRGYGNTARVLGRYVREQAVITLEE
                     AVRKMTSLPAGHFGFARRGELREGWAADLVVFDPEKVRDPATFEQPHHYAAGFEHVVV
                     NGVMVVKDGVVTGARPGQVLRREAGVSDAGSRGAGTGGRQEESGEKAPVLR"
     gene            3933..4718
                     /locus_tag="PKK95_02080"
     CDS             3933..4718
                     /locus_tag="PKK95_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC17022.1"
                     /translation="MTPASLYHSARLAAGYARARPPVHALIVEAVQRRFDELGIERAR
                     LALDLGCGTGLSTAALAPLASRAVGLEPALAMLRHGADVAPGARLVAGRAEELPFRDG
                     AFGLLAAAGSLNYTNLAAVLPEAARVLCEDGAFIIYDFSAGRRLAGDPRLDRWFSSFE
                     CRYPFPPGYEMDVKSLAFSDAGLRLVRLEQFQVAVPMTAEAYEAYALSETNVEQAVRA
                     GILEDDIAAWCRETLREVFEGATRDVLFDAYVAYVRPELPSAA"
     gene            5155..5715
                     /locus_tag="PKK95_02085"
     CDS             5155..5715
                     /locus_tag="PKK95_02085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOC17023.1"
                     /translation="MTVVTTIRNGVDVQRLMTMVEDVKREPWKGHLEFKLRSEWQGGF
                     QARHTPGDYVIGDQKLKHQTSNTVTSDEPPEILGKDAGPSPTDLVLSGLAACLTVGYA
                     ANAAAKGIDIQELRLEIDANGDLQGFMNLNDVRPGLSDVSVKVHLKSNAPQEQLQELH
                     DYVTAHSPIRDTISNPVKVRSELVRG"
     gene            complement(5722..6129)
                     /locus_tag="PKK95_02090"
     CDS             complement(5722..6129)
                     /locus_tag="PKK95_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17024.1"
                     /translation="MPLDERAFFTEKPETRSARYQCPRCRRTGEFRIRWVRRLKRQSA
                     PPGADEADRRKFAKLRDYLLRLDDEVVCPSCGKKFEIPSQHSLMFVDQLAGLPDEQDL
                     EKEIAAASGEPEPARDAKPALPPRFTRKPTGWK"
     gene            complement(6327..7568)
                     /locus_tag="PKK95_02095"
     CDS             complement(6327..7568)
                     /locus_tag="PKK95_02095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOC17025.1"
                     /translation="MFARLQANWEGCAMYDAIVVGARCAGSPTAMLLARQGHRVLLVD
                     RAVFPSDTLSIHYIHQPGVARLQRWGLLGQVAASNCPPIRRLDFDTGPFVLSGPPAPA
                     DGVADGYAPRRTVLDKILVDAAAAAGAEVRERFTVEALLTDGTRVTGIRGREHSRTHP
                     VVEKARIVIGADGPHSLVARSVGAHKYNEKPVFTCAYYAYWRGVSVVSAELYARPDRM
                     IIAAPTNHGQVVVIVYWPIRAFQEMRTDIERHFLEAAALAPRLAERLAKGERVERFRG
                     TADLPNFFRKPCGPGWALVGDAGYHKDPITALGISDAFRDAELLAEAVHEGLSGSRPM
                     EEALAEYQRRRDQAAVPLYELTCQNAALQPPPPEVQALLAALRANPEQTSRFFGAMTG
                     AVPVADFFAPENLKRVVGEVA"
     gene            7698..8567
                     /locus_tag="PKK95_02100"
     CDS             7698..8567
                     /locus_tag="PKK95_02100"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOC17026.1"
                     /translation="MAIDSAALTKALDWNGLDRIAEPLASTVRGAYESQGAVGQQVKD
                     LAHGVWLGHPLHPVLTDIPVGAWTVALALDAAAADDPGMRRAARLAIGVGLVGALGAA
                     VTGLTDWSETGGRSRRLGLVHGLLNIAATGLMAASWLERGNGGRSAGLATGWAGYLIA
                     AGSAYLGGDLVFGRRIGVTHAAVEEPEEFTEVARSSDLAEGSMKRVRKDDVDVLLVRQ
                     GGKVCALAHSCAHMGGPLSEGTLKDGSVVCPWHGSEFSLDDGRVLRGPATQPQPRFEV
                     RERDGAVEIKAAD"
     gene            complement(8572..9504)
                     /locus_tag="PKK95_02105"
     CDS             complement(8572..9504)
                     /locus_tag="PKK95_02105"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOC17027.1"
                     /translation="MKRRVAIVGTGWVGSSVAISTLHSGVADELLLHDLREDVAEGEA
                     MDLAHGASFYPSATVRAVPASAIGESDVVVIAAGRGGRPGQSRLDLLRHNAEMVREIG
                     RQLIGYEGTIVMVTNPVDVLTRVVAEASELPPARAIGTGTMLDTARLRQVLGRILRVD
                     TRSVHAHVVGEHGDSEVVLWSAARVGAVRLRDWPHWDPARETEVAEDVRTAANEIIRR
                     KGATNHAIGLVTAHLLGSLLRGERRVLTVSRVQEGVAGLHGVALSLPAVVGSGGAADV
                     IEPEMNADERERLHRSAAVLRDAADSLSRRAAAP"
     gene            9649..10656
                     /locus_tag="PKK95_02110"
     CDS             9649..10656
                     /locus_tag="PKK95_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOC17028.1"
                     /translation="MTNMGISRREVLALGAMTPFAGTTLAGGAGGPAPATPAERRKEL
                     YGLLGDLPDRNRPVSGRKVAEAERDGYILETWVLDLNGLEAVPAHVARPRGATGRLPA
                     VLFNHSHGGGYGIGKKEFVEGRSYLQPKPYAKELTDLGYVALAIDHWIFGDRSHTSEL
                     DMFKLMLWRGQVLWGMMVYDSLRALDLLLQRPDVDPARVATLGMSMGSTMAWWVAALD
                     ERVKVTVDINCLTDFDSLVADKGLARHGIYYYVPGLLKHFTTSQINALIAPRPHLSLV
                     GLRDALTPLEGVERIDRELTAEYARLGHPERWKLLRYDVEHQETPEGREAIVAFFREW
                     L"
     gene            complement(10770..11474)
                     /locus_tag="PKK95_02115"
     CDS             complement(10770..11474)
                     /locus_tag="PKK95_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOC17029.1"
                     /translation="MTANRPARSLTVSAEDYLKAIYTLSADAPASTTEIAQRLDLAPA
                     SVTGMIRRLSSQKLLDHQPYRGVVLTPAGRRMALRVLRRHRVIEAYLAKFLGYRMDDV
                     HAEADRLEHAASDALVERMAAALGHPRVDPHGDPIPDADGRLDKVTYTALADLPLGQD
                     AQLRRVTATEPERLRYLAEAGLVPGARVCLVDRQPFDGPVTVRVAGAERTLGREMARL
                     LLCDLTVGPGRAARRQ"
     gene            complement(11488..13644)
                     /gene="feoB"
                     /locus_tag="PKK95_02120"
     CDS             complement(11488..13644)
                     /gene="feoB"
                     /locus_tag="PKK95_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOC17030.1"
                     /translation="MNPLAAASACHGCAHRGESLIQLGVVPGRWDYLVALAGNPNTGK
                     STVFNALTGLRQHTGNWPGKTVARAEGTFRHGGKAIKIVDLPGTYSLQAASTDEEVAR
                     DFLLFGRPDVTVVVIDATRLERNLNLALQVLEITDRVVVVLNLMDEARRHGIGVNTDA
                     LARELGVPVIPAAARQGTGIGALRDAILQVATGAARPQPHRLRRLPADVESPIAEVLP
                     AVEAAYPDTLGKRWVALRLLNGDERVLRAVPSGELGAKIVEARWRLPGDFQDRLTGAL
                     YAEAERIARASEVTGLRRVRVDLDKHLDRLLTSRWTGFPLMVLLLAVVFWLTIAGANV
                     PSSMLASLLLDDVHPVLKGFGAAVGMPWWMSGFLFDGMYLATAWVVSVMLPPMAIFFP
                     LFTLLEDFGYLPRVAFNMDRMFQRVGAHGKQALTMCMGFGCNAAGVVATRVIDSPRER
                     LVAIITNNFSLCNGRWPTQILIASIFLGTLVPAHLAGITAAGAVVAIALLGVFFMFAV
                     SWGLTRSVLKGEATTFSLELPPYRPPRILQTLYTSLIDRTAIVLWRAVVFALPAGAAI
                     WLISNVHLGGGASIAATLVSALDPVGVLIGLNGVILLAYVVAIPANEIVIPTILMLTV
                     HTLRLAGLGAGDGVMFELDDSGAVRQLLVGAGWTTLTGINLMLFSLLHNPCSTTIYTI
                     YKETGSARWTAVATLLPLALGVSVCFLVAQVWRLLF"
     gene            complement(13641..14681)
                     /locus_tag="PKK95_02125"
     CDS             complement(13641..14681)
                     /locus_tag="PKK95_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOC17031.1"
                     /translation="MSPLPDPAAALAIFGSLVLLAAILFWPERGIVPRLRRLVELSER
                     VRLEDAVKHLFNSAAAGRTASVDSLAGAVGVSRDRTVEVARELTRLGLARLEGEVIAL
                     TEAGRAHALRVIRTHRIWERYLADRTGVGAAEWHALADRLEHKASADEVDRLAASMGD
                     PVYDPHGDPIPTASGVLPPARGLPLSAVAAGASARVVHIEDEPGQVYRSLRAAGVNVG
                     CLVRLDRVDPAGLHLLADGRPVVIDSLAALNLVVELVEEDDTEEGLERLDAIRPGEEA
                     IVVRLGAQIHGAQRRRLLDLGIVPGTVVRAELSSLSGDPVAYRIRGAVIALRRAQADD
                     IVVRRRPRKEAA"
     gene            complement(14738..16300)
                     /locus_tag="PKK95_02130"
     CDS             complement(14738..16300)
                     /locus_tag="PKK95_02130"
                     /inference="COORDINATES: protein motif:HMM:NF039677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HOC17032.1"
                     /translation="MGRRVDLARLLETARLERVVPRLRAETLHRLIQYRGLESSGEVI
                     AAATPEQISSVLEVDLWRRVGAGGDERFDPGRFGEWLGALVEMGDEVAARVVSAIDER
                     LVTAGLSGHVKVFDPAARDAPLTPGNLATEVGGYLVSARRTDAWDAIVSLLLALDAAH
                     HPFFESVMRGCRALSNSVPEADGLHELLSAPGQLLHDLASVREARRSGQGYLSPADAR
                     LVLDLARKPFGDPTDAASIRRVFADYALHGAGQQGEVGAALPGRPDAVGKAPEAPAAG
                     TDAPIEALIRLLAEEGLVPERPRALLPGTVGEASRLERMRELMARVRDAGDEAYEARS
                     RELAFLANALVAGCSIHARPFAPQEASDAVVATCNLGLEEWGSLHAGFLVGHDLLSVF
                     HRGWRALHEMSLSAADRLIAILGVLRCADLETQAGLHVLRQQLAKHRAAGAPWRARDA
                     LDVIAVLDLPAWVSLLGVLDECPIVPAALIATLERRTGSISATAFEFVSTRRQVGDVR
                     AFMAKLPDILRR"
     gene            16496..18478
                     /locus_tag="PKK95_02135"
     CDS             16496..18478
                     /locus_tag="PKK95_02135"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC17033.1"
                     /translation="MFAPPLSRVLRPPGSRLKRYAIAVALVGAATLFRAAIEPLVGTT
                     VPFGLFVPTVIVAAWLGGAEGGLLATALAALAGKFLFIEPRYHATFTTRDIAIVLLAG
                     AVGSGLVLLVSVWRRTQLRLLDLHDEMAGILRQLPAGVVVADRSGRVVFVNTELERLL
                     GGPLRLGMFADFRRQYTPLHADGTAIALSESPLLRAANGEEVPEEEATIVRSDGERRI
                     VRVRGTPVRRHDGTVAGGAVTVVDVTELRETARVARERAEELGQADARLREQARELER
                     VNRLKDEFLATLSHELRTPLTAIVGWAAMLLRGGLSPDVVHRAHETIARNADSQRALI
                     EDVLDVSRIVTGNLRLEKGPADVEGPLRAAVEAVKPQAEEAGVTITTALTDTSTAVWG
                     DSVRLQQVFVNLMSNAIKFSGRGGRIWVSLRRVGAKVEVQVRDNGIGISAEHLPHIFE
                     RFRQADSSTTRRHNGLGLGLAIVRHITGLHDGTVKADSPGEGQGATFTVCLPYHPLEE
                     GAPTATSPPRPGVFAGPAAPPSPGSTPTLRGRRVLVVDDEADTRQLVASVLTASGAEV
                     ISAASAREALEQLQAGVPDAMLVDISMPEMDGYEFVRAIRNSRSGAEHVPAIAFTAHA
                     REEDRRLALTSGFRMHVAKPVEPTALVRALATVLER"
     gene            18503..20743
                     /locus_tag="PKK95_02140"
     CDS             18503..20743
                     /locus_tag="PKK95_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC17034.1"
                     /translation="MPLQTGTRLGPYEIVELLGAGGMGEVYRARDPRLGRDVAIKVMT
                     ALSDRSQALRFQQEARAVAALSHPNVVAIFDVGGDERPFLVTELLRGQTLRSLVERGP
                     VPLAEAVELSLQAAAGLGAAHEMGIVHRDLKPENLFLTADGRLKILDFGVAKMVSADG
                     GVTRTATTSPGMVLGTVGYMSPEQVRGLEADARSDIFALGAVLYELLTARRAFDGPTP
                     ADRLAAVLHESPAALHFPAAVPASLVSLVQRCLQKAPEARFQSAREFALAVEGLTLAQ
                     PTAAVGAGNAGHSSIAVLPFANLSADPDNEYFSDGLAEDLTSALARLAPLRVAASTSA
                     FRFRRGGRDLREVGRELGVETVLEGSVRRAGGRLRITTRLVDVASGYQLWADRYDRKL
                     ADVFDVQDEIVESIVQAIAPTLVPVARAAAHRGTRNLEAYELYLKGRYFWNQRSPAVL
                     PAALRSFEEAVARDPDYALPYCGLADCYTILHAYGWMPPAMCRPKAEQSVERALALEP
                     GLPEARLSRALYVFHFERHWRQARRSFAEALEARPRMAIGQVQYALFLATAYEFQEAR
                     ERIDLALGLDPDASIIHFLAASACCLTGDFEGAERHATRALELQPESLGARWPLTIAL
                     LATGRTSDAIAVGETVVARSRAPVFLGVMSMVYGRAGRLADAQRLARELDERRARGEF
                     VVPFADLGVHLGLENRAGVRDALAACVDGGAAPMAVAGPTRFLLDGYRSDPEIALLLD
                     RLHDVE"
BASE COUNT         3157 a         7302 c         7288 g         3090 t
ORIGIN      
        1 cgatcgagtg gcggcgcgtg cccgcggccc catcacggcc ggccttcgag atcgccgcca
       61 ccgagacgac ggtcggccag tttcgcgcct tcagccgcgc caccgcgacg ccgcttccgc
      121 ggcagccgca ctggagcgac gaggaccatc ccgtggtcaa cctcacctgg gccgaggcgt
      181 cggcgttctg cacgtgggcg ggcggccgtc tcccgaccga ggccgagtgg gagtcggccg
      241 catcggcggg cggaacgggg cagtacggat ggggcgacac gtgggatccg tcccgcgcga
      301 acgcggccgg cgtcgccggc aacgacgtgt ggggatacac ctcgccggtc gcgtcgttcc
      361 cgccgaacgc tctcggcctc tacgacatgg tgggcaacgt gtgggaatgg accgccgacc
      421 tgtactcccc cgagcccgcg aaccgcggac gccgctccct gcgccgtgcc gtccggggcg
      481 ggtcctgggc caatcgccgc gaaaacgtga ggctgtcctg ccgcgacggc ctgtccgagt
      541 cagggcgcca caacctccac gtcggcatcc gctgcgtgcg ataggcaggc tcagcgctcg
      601 agactcgcgt cccttgtgta agcgcgaggc acgcgatggc acgatgcctg taagcgtgcg
      661 ggcaaggagt cacgcatgaa gaagagcgaa ctgccctcga cgctgaagcg ctcgtcaccc
      721 aaggcgcagc gcacctacgc gaagacgcac gacagcgcgg tccgcagcta cggcgagggc
      781 gagcgagccc accggacggc catctcgtcg ctcaagcact cgttcgagaa ggtcggcgac
      841 cgctgggagc ccaagaagaa gagaggcccc tccgacccgc gcgccgcgaa gccggcaagc
      901 gaggcgcgcg ccgggaaggg tgaaacgttc ggcggcgtcg attacgccgg gcgttcgaag
      961 ggggagctgt acgagcgggc gcgcgcgctg cagatcggcg ggcggtcggg gatgtcgaag
     1021 gaagagctgg cccgggcgat tgcgaagaaa cagcggtgag agggcgctgg aggcgggagc
     1081 cgcgccgagg cgttgccggg cagggaatcg ggcaggagcg cccggatgaa cgctcctgcc
     1141 ccaggtgcta tcgctcgcgg cgcaactgct cgtcgcgccg gcagtcgggg cagagcatcg
     1201 gcacgatgtc ccgcaccatc ggctgggtgc cctcgagcga cttccagccc gacaggatca
     1261 accggtcgat gtcctcgtgc accacgaaga tctgacagcg agtacagcgt cccttgacca
     1321 tcaactcatc cctttcaaat gaagcgacac cggtcccatc tcttaagacg ccggaaccgg
     1381 ggaaaaggat tccgcctcgg ccttagaccc cggtcttctc catgctgggg ttccacacct
     1441 tccagacgtt gccgaccagg tcgggccccg gcgtcagcgt cggctgcccg ggctcccatc
     1501 cggccggggt ggcttcggtc cccttgctgt tccgcacgtg ctggtaggcc tggatctggc
     1561 gaacggtttc gccgaacttc cgccccaccg gcggcgtcag cacctccatc gcctggataa
     1621 cgccgtcggg gtcgatgatg aaccggcccc gcagctcgat accctgctgc tcatcgtata
     1681 cgccgtacgc ccgcccgacg tttcccccct ggtccgaaac catgtggaag gggacgcccc
     1741 cctccaccat cctgaccagc tcatggtcgt tccacatctt gtggacgaag tggctgtcca
     1801 cgctgaccga gagcacctcg acgccaagtt cctgcaacac cgggtacttg tcggcgaccg
     1861 ccgacacttc ggtcgctcaa acgaatgtga agtcgccggg gtagaagcag agcagcaccc
     1921 acttgccaag caggtccgac agcttgacgt tggtgaagct cccccggtaa taggctggcg
     1981 cctggaagtc gggcgccttc tgtccgacgc gggcagtcat catcgcagcc ctccgtggct
     2041 cgacgggttc gggcccggca gcttcgggcg gcggttcgcc gaccaccgct ccagtcgtcc
     2101 gggcacatcc ttttggtgct tctgccatgg gcacctttca ggcccgtgtg ggccggttat
     2161 tggatgaggt aagtgcccct tcatcctatc tcaaaacagg cgctttttcg ccactttcct
     2221 cttgcctgcc gcctgtcccg gctccccggc tgcctgcgtc cgacacccct gcctcgcgcc
     2281 gcagaacctg cccgggccgc gcgccggtga ccaccccgtc cttgaccacc atcaccccgt
     2341 tcacgacgac gtgctcgaac ccggccgcgt aatggtgcgg ctgctcgaag gtggccgggt
     2401 cgcgcacctt ctccggatcg aacacgacca ggtcggccgc ccacccctcg cgcagctcgc
     2461 cccgccgggc gaaaccgaaa tgcccggccg gcagcgaggt catcttccgc acagcctcct
     2521 cgagggtgat taccgcctgc tcgcgcacgt agcggccgag aacgcgtgcg gtgttgccgt
     2581 acccgcgcgg gtgcggcgtg ccctcccccg gccgcagcac gccgctgtcg gacgcgatca
     2641 tgacgttcgg atcgcgcatg atccgcttca cgtcgtcctc ggacatgaag tggtagacca
     2701 tctgcggctc ggagccgagc atcaggcgcc gtgccgtctc gagctgtgcc tcgaggtcgt
     2761 ccttgccgag cagcctggcc gctgcctgct tcacggtgag accgttgagc gacgggtcgt
     2821 ctcggtcgag ggcgatgacc gcccaggcaa gatcggtgaa gccgcgctcg gcgagcagcg
     2881 ctttcatccc gtccttgacg cgccgccagg tggcttcgtc ccggagccgc gcctcgatgg
     2941 cggggcgccc tccctcgagc gcccaccccg ggaagcggat gccaaggccc gcggaaccgg
     3001 ccgtgtaggc gtattggtcg gcacgcacgt ccacgccgcg ccggcgtgcc gacgcgatcg
     3061 catcgagcag cgcctggctg gttccccagc ggttcggact atcgatcttg aggtgtgaga
     3121 cctggacggg gcaggacgcc gcctcgccga tcgcaatcgt ctcggccacg gcctcggcga
     3181 ccctcgtccc ctcgttgcgc atgtggctgg cgtacagccc gccggcgcgg gcggccacct
     3241 tcgccagctc gatgatctcg ccgcgctcgg cgtactgccc cggaacgtac tgcagccctg
     3301 tcgagaggcc gatagcccct tcccgcatcg ccttcgcgac tagggccttc atccgctcga
     3361 gctccgcggg ggtcggggcc cgtccggccg cccccatgac ggcggcgcgt accgtgttgt
     3421 gcccgacgag ggtcgagaag ttgaccgcaa ggccctcgcg agtgatggcc tgcagctccg
     3481 aggccacgct ctcggccgag tcgccgcagt tgcccgcaac gagcgtggtg acgcccattc
     3541 ggacgaagtt ctcgacgagc ggagttcgga cggggccgtc ggcgtgcgtg tgcacgtcga
     3601 tgaagcctgg cgcgacgctg cgccccttcg cgtcgatgac ggccttcgcc gagacgcccg
     3661 gcaggcggcc gatgcgggcg attcggcccc cgctcaccgc caggtccgcg gcgatgccgg
     3721 ggcggccggt cccgtcgacg agggtgccgt tcacgatgag caggtcgtag gcctcgggct
     3781 gctggcgcgc acggccgggc ggcgcagccg acaggcaggc aacggcggcg gcgagggcaa
     3841 ccagcgagcg gaagtggaag ggcatgaggg aggttgtaca cagcgcgcgg gcccgagggc
     3901 aagcgtcgtc ggcgcgtgct accatcgggc cgatgacccc ggcgagcctg taccacagcg
     3961 ccaggttggc ggccggctac gcccgggcac gcccgcccgt gcacgcgctc atcgtggagg
     4021 ccgtgcagag gcgcttcgac gagctcggca tcgagcgggc tcgcctcgcg ctcgatctcg
     4081 gctgcggcac gggactctcg accgccgcac tcgcgccgct cgcctcgcgt gccgtcggcc
     4141 tcgaaccggc ccttgccatg ctgcgccatg gcgccgacgt ggcgcccggt gcccgcttgg
     4201 tcgccgggcg cgccgaggag ctgccgttcc gggacggggc cttcggcctg ctcgcggccg
     4261 cgggctcgct caactacacg aacctcgcgg ccgtcctccc cgaggcggcc cgcgtgctct
     4321 gcgaagacgg cgccttcatc atctacgact tctcggccgg ccgccggctg gccggggatc
     4381 cccggctcga ccgctggttc tcgtccttcg agtgccgtta cccgtttccg cccggctacg
     4441 agatggacgt gaagagcctg gcgttctcgg acgccggcct gcggctcgtg cgcctcgagc
     4501 agttccaggt tgccgtgccg atgacggctg aggcctacga ggcgtacgcc ctcagcgaga
     4561 ccaacgtcga gcaggccgtg cgcgccggca tcctggagga cgatatcgcc gcgtggtgcc
     4621 gcgagacgct gcgcgaggtc ttcgaagggg cgacgcgcga cgtgctgttc gacgcgtacg
     4681 tcgcctacgt gcgccccgag ctgccgtcgg ccgcctgacc tcgttccgct tccccttctc
     4741 cgcggggccg gcccggcgca ccccgaccct ctctagccat ccgggcggcg cccctcgccc
     4801 ttgccttgcg ggcttcttcg tcccggcttc gcggcctcgc gctcgtcgtc agcgagcgga
     4861 ccaggcgggt agccgcctcg gtgacgtcct ccaccgcgcg atcgaacact gcctggttca
     4921 ccttggacgg ccggctggta ccgctcagct tccgaacgaa ctgcagcgcc gaggcgcgaa
     4981 tctcatccgg cgtggcgggt ggttcgaagt tcgcgagcgt cctgatgttc ctgcacatgg
     5041 gtgcgccttt tgctcctatc agctcccgac cctccgacgc gtatcaacat caatcactcc
     5101 ggctcacgcc gcatcgcggg ctgttggagc cggagacagt aaagaggagc ccccatgacc
     5161 gtggtgacga cgatccggaa cggcgtcgac gtccagcgcc tcatgacgat ggtggaggac
     5221 gtcaagcggg agccttggaa gggccacctg gaattcaagc tccgatccga gtggcagggc
     5281 gggttccagg cgcgccacac gccgggtgac tacgtgatcg gcgaccagaa gctgaagcac
     5341 caaacctcga acaccgtgac cagcgacgag ccgcccgaga tactgggcaa ggacgcgggg
     5401 ccgagcccga ccgatctcgt gctgtccggc ctcgctgcct gcctgaccgt cggctacgcc
     5461 gccaacgccg cggccaaggg catcgacatc caggagctgc gcctcgagat cgatgcgaac
     5521 ggagacctgc agggcttcat gaacctgaac gacgtgcgcc cggggctttc cgacgtgtcg
     5581 gtgaaggtgc acctcaagtc gaacgcgccg caggagcagc tgcaggagct gcacgactac
     5641 gtcacggccc actcgccgat ccgcgacacc atcagcaacc ctgtgaaggt caggtcggag
     5701 ctggttcgcg ggtaggctcg actacttcca acccgtcggc ttgcgtgtga acctcggggg
     5761 aagggcgggc ttcgcgtcgc gcgcgggctc gggctccccc gaggccgccg cgatctcctt
     5821 ctcgaggtcc tgctcgtcgg ggagccccgc cagctggtcc acgaacatca gcgagtgctg
     5881 cgagggaatc tcgaacttct tgccgcagct cgggcacacg acctcgtcgt cgaggcgcag
     5941 gaggtagtcg cggagcttgg cgaacttcct gcggtcggct tcgtcggcac ccggaggggc
     6001 gctctgcctc ttcagacgcc gcacccaccg gatgcggaac tcgcccgtcc gccggcatct
     6061 cgggcactgg tatcgggccg acctcgtctc gggcttctcg gtgaagaagg cgcgttcgtc
     6121 cagcggcatg cgcaatatct tacgcctccg atgcaggatc ctgccatgcg ccggcttcct
     6181 cgagaggccg tctacgcccg caatattcac gaacgctgcg cgttcgttca taacgcgccg
     6241 gcggcacagg cgttggacct gccgccggtt tcgacaccgc gctgcgcgcg gtgctcaaga
     6301 cgtcgcccgg gcgaggatgt tccgggctac gccacctcgc cgacgacgcg tttcaggttc
     6361 tcgggagcga agaagtcggc caccgggacg gcgccggtca tcgccccgaa gaaccggctc
     6421 gtctgctcgg gattggcgcg cagggcggcc agcagcgcct gcacttcggg cggcggcggc
     6481 tggagcgcgg cgttctgaca ggtcagttcg tacagcggaa cggcggcctg gtcgcgtcgc
     6541 cgctggtact cggcaagcgc ctcctccatc ggacgcgaac cggagagccc ctcgtgcacg
     6601 gcctcggcca gcagctccgc gtcccggaac gcgtcgctga tgccgagcgc cgtgatcggg
     6661 tccttgtggt agcccgcgtc gcccacgagc gcccaacccg ggccgcacgg cttccggaag
     6721 aagttgggaa gatcggcggt gccccgaaaa cgctcgaccc gctcgccctt cgccagccgc
     6781 tccgccagcc tgggcgcaag cgcggccgct tcaaggaagt gccgctcgat gtcggtccgc
     6841 atctcctgga aggcgcgaat gggccagtag acgatgacca ccacctggcc atggttggtc
     6901 ggcgcggcaa tgatcatccg gtcgggcctg gcgtacagct cggcgctcac gacgctcacc
     6961 cctctccagt aggcgtagta cgcgcacgtg aagaccggtt tctcgttgta cttgtgagcc
     7021 ccgacgcttc gcgccacgag cgagtgcggg ccgtcggctc cgatgacgat ccgcgccttc
     7081 tcgaccacgg ggtgcgtgcg ggaatgctcc ctcccgcgga tacccgtcac ccgggtcccg
     7141 tcggtcagca gcgcctccac cgtgaagcgt tctctcacct ccgcccctgc cgcggccgca
     7201 gcatcgacga ggatcttgtc gagcaccgtg cgccttggag cgtagccgtc ggcgaccccg
     7261 tcagccggcg ccggaggtcc cgacagcacg aacggtcccg tgtcgaagtc gagccttcga
     7321 atcggcgggc aattacttgc ggcgacctgg cccagcagac cccagcgctg gagccgggcg
     7381 acgccgggct gatggatgta gtggatcgac aacgtgtcgc ttggaaagac agccctgtcc
     7441 accaacagta cgcggtgtcc ttggcgggcc aggagcatcg cggtcggcga tccggcgcag
     7501 cgcgcaccga caacgatcgc gtcgtacatc gcgcacccct cccagttggc ttggaggcga
     7561 gcgaacatgc aagccaccgc acgtcagcgg ttcgcttctt gcgtcgtcgc aagcgggacc
     7621 gttgctcagt tgacgcgagc tcacctcctg ggttgaccac gttcgctccg ggggcggcga
     7681 aggcggtgga ggatgcgatg gcgatcgatt cagcggcgtt gacgaaggcg ctcgattgga
     7741 acgggcttga ccgcatagcc gagccgttgg cgagcacggt gcgcggtgcg tacgaatcgc
     7801 aaggagcggt cgggcagcag gtcaaggacc tcgctcacgg tgtgtggctg ggccaccccc
     7861 tccacccggt tctgacggac atccctgtcg gggcgtggac cgttgcgctt gccctcgatg
     7921 ccgcggccgc agacgaccca gggatgaggc gcgcggcccg gctggcaatc ggcgtcggcc
     7981 tggttggggc gctcggagcg gccgtgaccg gcctcactga ctggtcggag accggcggcc
     8041 gctcgcgccg cctcggactc gtccacggcc ttctgaacat cgccgctacc ggcctgatgg
     8101 ccgcgtcgtg gctcgagcgc ggaaacggcg gccgctcggc gggcctcgcg accggctggg
     8161 ccggttacct catcgcggcc ggctcggctt acctcggagg cgatctcgtc ttcgggcgcc
     8221 gcatcggtgt cacccatgca gcggtcgagg agcccgaaga gttcaccgag gtggcgcgga
     8281 gcagcgatct cgccgagggc tcgatgaagc gcgtccggaa ggacgacgtg gacgtgctgc
     8341 tcgtgcggca gggcggcaag gtgtgcgcgc tcgcccactc ctgcgcccac atgggaggac
     8401 cgctctcgga aggcacgttg aaggacggtt cggtggtctg cccgtggcac ggctcggagt
     8461 tctcgctcga cgacgggcgg gtgttgcgcg ggccggccac gcagccccag ccccgcttcg
     8521 aagtccgaga acgggatggc gcagtcgaga tcaaggcggc cgactgaacg attacggtgc
     8581 ggcggcacgc cgtgagaggc tgtcggcggc gtcgcggagc acggccgccg agcggtgcag
     8641 gcgctcgcgc tcgtcggcgt tcatttcggg ctcgatcacg tcggcggccc cgccggaacc
     8701 gacgacggcc ggaagggaga gcgcgacgcc gtgcaggccg gccacccctt cctgcacgcg
     8761 ggagaccgtg aggacgcgac gctcgccgcg gagcagcgaa ccgagcaggt gcgctgtcac
     8821 gaggccgatc gcgtggttgg tggctccctt gcgccggata atctcgttcg cggcggtgcg
     8881 gacgtcctcg gcgacttccg tctcgcgggc aggatcccag tgaggccaat cgcgcagccg
     8941 cacggccccg acccgcgcgg ccgaccagag aaccacttcc gaatcgccgt gctcgcccac
     9001 gacgtgggcg tgcaccgatc gcgtgtccac ccgcaggatg cgcccaagaa cctgcctgag
     9061 cctcgccgtg tcgagcatcg tccctgtgcc gatggcgcgg gctggcggaa gctccgacgc
     9121 ctcggccacc acccgggtca gcacgtccac cgggttggtc accatcacga tggtgccctc
     9181 gtagccaatg agctgtcggc cgatctcccg gaccatctcg gcattgtgcc gcagcaggtc
     9241 caggcgcgac tggccgggcc tcccgccccg gccggcggcg atcacgacga cgtccgactc
     9301 cccgatggcc gaggcgggaa ccgccctcac cgtggcgctc gggtagaacg acgcgccgtg
     9361 ggcgaggtcc atcgcctcgc cttcagccac atcctctctg aggtcgtgca acagcagctc
     9421 gtcggcgacg ccggagtgga gcgtcgagat tgcgacgctc gatccaaccc agcccgtccc
     9481 tacaatcgcc acgcgtctct tcatcgtccc tcggtgtgct ctcgctgtcc tcgaaacgct
     9541 cgatgcaaat cgtcgtccgc gacacgggcc tggcccggct ccattgggcc gacgatggtg
     9601 ctcgaaggga tgggacgatt atcccacggg gcgagtagta tgtcggccat gacgaacatg
     9661 ggcatcagca ggcgcgaggt gttggcgttg ggtgcgatga caccgtttgc gggaacgacg
     9721 ctcgccggtg gtgcgggcgg gccggcgccg gcgacgccgg ccgagcgccg caaggagctg
     9781 tacgggctgc tgggggatct gccggaccgc aaccgcccgg tgtcgggacg gaaggtcgcg
     9841 gaggccgaac gcgacggcta catcctcgaa acatgggtcc tcgatctcaa cgggctcgaa
     9901 gcggtgccgg cgcacgtcgc ccggcctcgc ggggccaccg gccggctgcc ggccgtgctc
     9961 ttcaaccact cgcacggcgg cggctacggc atcggcaaga aggagttcgt cgagggacgc
    10021 agctacctgc agccgaagcc ctatgccaag gaactgaccg acctcgggta cgtggccctg
    10081 gcgatcgacc actggatctt cggcgaccgc agccacacgt ccgagctgga catgttcaag
    10141 ctgatgctgt ggcgcggcca ggtgctgtgg ggaatgatgg tctacgacag ccttcgcgcg
    10201 ctcgacctgc tgctgcagcg gccggacgtg gacccggcgc gcgtcgccac gctcggcatg
    10261 tcgatgggca gcacgatggc ctggtgggtg gcggcgctcg acgagcgggt gaaggtgacg
    10321 gtggacatca actgcctcac cgacttcgac agcctcgtcg ccgacaaggg gctggcgcgg
    10381 cacggcatct actactacgt gccgggcctg ctgaagcact tcacgacttc gcagatcaac
    10441 gcgctgatcg cgccgcggcc gcacctgagc ctggtcggct tgcgtgacgc gctgacgccg
    10501 ctcgagggcg tcgagcgcat cgatcgcgaa ctgacggccg agtacgcacg ccttgggcac
    10561 ccggagcggt ggaagctgct gcgctacgac gtcgagcacc aggagacgcc cgaaggacgt
    10621 gaggccatcg tggcgttctt tcgggaatgg ctgtaagtcg gagcacgggg ggccgcatgg
    10681 ccattcgccc gctacgggtg gtgctcgtcg tggtggctgt cctgtcggcg gctccggtcg
    10741 gggctcaggt ccaggagact ggttcggcgt cactggcggc gtgccgcgcg gcccgggccg
    10801 acagtaaggt cgcacaacag gagccgcgcc atctccctgc cgagcgtgcg ctcggccccg
    10861 gcaacccgca cggtgaccgg gccgtcgaac ggctgtcgat cgaccaggca aaccctcgcg
    10921 cccggcacga ggcctgcctc ggcgaggtag cgcaagcgtt ccggctcggt ggccgtcacg
    10981 cggcgcagtt gggcgtcctg cccgagcggc aggtcggcga gcgccgtgta cgtcaccttg
    11041 tcgagccggc cgtcggcgtc gggaatcggg tcaccgtgag gatcgacccg cgggtggccg
    11101 agggcggccg ccatccgttc gacgagcgcg tccgaggctg cgtgctcgag gcggtctgct
    11161 tccgcgtgca cgtcgtccat ccggtacccc aggaacttcg ccaggtaggc ctcgattacc
    11221 cggtgacgcc gcagcaccct caacgccatc ctgcggcccg cgggggtcag cacgacgccg
    11281 cggtagggct ggtgatcgag cagcttctgg cttgagagcc ggcggatcat cccggtcacg
    11341 gaggccgggg cgaggtcgag ccgctgcgcg atctcggtcg tcgaggccgg ggcgtcggcc
    11401 gagagcgtgt agatcgcctt caggtagtcc tcggccgaaa cggtcagcga tcgagccggg
    11461 cggttcgccg tcatcgacac cccctcgtca gaacagcagg cgccagacct gcgccaccag
    11521 gaagcacacc gacacgccga gggccagggg caggagcgtg gcaaccgccg tccaccgcgc
    11581 gctgcccgtt tccttgtaga tcgtgtagat cgtcgtggaa cacgggttat ggagcaggct
    11641 gaagagcatc aggttgatgc cggtcagcgt tgtccacccg gcgccgacga gcaactgccg
    11701 caccgccccc gagtcgtcca gctcgaacat cacgccgtcg ccggcgccca ggccggccag
    11761 ccgaagcgtg tgcaccgtga gcatgagaat cgtggggatg acgatctcgt tggcggggat
    11821 cgccaccacg taggccagga ggatgacacc gttcaggccg atcaggacgc cgaccgggtc
    11881 gagcgcgctc accagcgtcg cggcgatgct cgccccgccg cccaggtgaa cgttcgagat
    11941 cagccagatc gcggcgcccg ccggcagcgc gaagacgacg gcccgccaca gcacgatcgc
    12001 cgtgcggtcg atcagcgagg tgtacagcgt ctgcaggatc ctgggcgggc ggtacggcgg
    12061 aagttccagt gagaaggtcg tcgcctcgcc cttgagcacg ctgcgcgtca gcccccacga
    12121 caccgcgaac atgaagaaga cgccgagcag ggcgatcgcc acgaccgcac cggcggcggt
    12181 gatcccggcg aggtgcgcgg gaaccagggt gccgaggaag atgctcgcga tcaggatctg
    12241 cgtcggccag cgcccgttgc acagcgagaa gttgttcgtg atgatcgcga ccagccgctc
    12301 gcgcggcgag tcgatgacgc gcgtcgcaac cactccggcg gcgttgcacc caaaccccat
    12361 gcacatcgtc agcgcctgct tgccgtgcgc accgacgcgc tggaacatgc ggtccatatt
    12421 gaaggcgacc cgcggcaggt agccgaaatc ctcgagcagc gtgaacagcg ggaagaagat
    12481 cgccatcggc ggcagcatca ccgacaccac ccacgccgtg gcgaggtaca tcccgtcgaa
    12541 gaggaagccg ctcatccacc agggcatccc gacggcggca ccgaagccct tgagaacggg
    12601 gtgcacgtcg tccagcagca gcgacgccag catggaggag gggacgttgg cgccagcgat
    12661 cgtcagccag aagacgacgg ccagcagcag caccatcagc gggaagccgg tccaccggct
    12721 cgtcaggagc ctgtcgaggt gcttgtcgag atcgacgcgc acacggcgca ggccggtcac
    12781 ctccgaggcg cgcgcgatcc gttccgcctc ggcgtacaac gctcccgtca gcctgtcctg
    12841 gaagtcgccc gggaggcgcc agcgcgcctc cacgatcttc gcgccgagct cgccggacgg
    12901 aaccgcgcgc agcacccgtt cgtcgccgtt caggagccgc agcgccaccc agcgcttgcc
    12961 caaggtgtcc gggtaggccg cctcgacggc gggcagaacc tcggcgatcg gggactcgac
    13021 gtcggcgggg agccggcgca ggcggtgggg ctggggccgc gcggcgccag tcgccacctg
    13081 caagatcgcg tcgcgcagcg cgccgatccc ggtcccctgc cgggcggcgg ccgggatgac
    13141 cgggacaccc agctcacggg cgagcgcgtc ggtgttcacg ccgattccgt ggcggcgcgc
    13201 ctcgtccatg aggttgagca ccaccacgac ccggtcggtg atctcgagca cctgaagggc
    13261 gagattgaga ttcctctcca gccgggtggc gtcgatcacg acgacagtga cgtcgggccg
    13321 gccgaagagg aggaagtcgc gcgcgacctc ctcgtcggtg ctcgccgcct ggagcgagta
    13381 ggtgcccggc aggtcgacga tcttgatggc cttcccgccg tgcctgaacg tcccctcggc
    13441 cctcgccacc gtcttgcccg gccagttgcc ggtgtgctgc cgcaaaccgg tcagggcgtt
    13501 gaagaccgtg ctctttcccg tgttcgggtt gcccgccagg gcgaccaggt agtcccatcg
    13561 ccccgggacg actccaagct ggatcaggct ctcgccgcga tgggcgcagc cgtggcaggc
    13621 agaagcggcg gccagcgggt tcacgcggcc tcctttcgcg ggcggcggcg aaccacgatg
    13681 tcgtcggcct gtgcccgccg aagcgcaatc accgccccac ggatccggta cgccaccggg
    13741 tcgcccgaca ggctgctgag ctcggcccgg acgacggtgc caggcacgat cccgaggtcg
    13801 agcaggcggc ggcgctgggc accgtggatc tgcgcgccaa ggcggaccac gatggcctct
    13861 tctccagggc gaatggcgtc caggcgttcg aggccctcct ccgtgtcgtc ttcctccacc
    13921 agctcgacga cgaggttgag ggccgcgagc gagtcgatca ccaccggccg gccgtcggcg
    13981 aggagatgca ggcccgccgg atcgacccga tccagccgca cgagacaccc gacgttcacc
    14041 ccggcggccc tcagcgatcg gtaaacctgc ccgggttcgt cctcgatgtg caccacgcgg
    14101 gcgctggcgc cggctgcaac ggctgagagc ggcaggccgc gggccggcgg aaggacaccg
    14161 gaggccgtgg ggatgggatc gccgtgaggg tcgtacaccg ggtcgcccat gctggcggcg
    14221 aggcggtcca cctcgtcggc cgaggccttg tgctcgagcc gatcggccag cgcgtgccat
    14281 tcggcagccc ccacgccggt gcggtcggcc aggtagcgct cccagatgcg atgggtgcgg
    14341 atgacgcgga gggcatgggc acggccggcc tcggtgagcg cgatgacctc gccctcgagc
    14401 ctggcgaggc ccaggcgcgt caactcgcgg gcgacttcga cggtgcggtc gcgcgagacg
    14461 ccgacggccc cggccaggct gtccaccgac gccgtgcggc cggccgcagc gctgttgaag
    14521 aggtgcttga cggcgtcctc gagccgcacg cgctcggaaa gctcgaccag gcgccgcagg
    14581 cgcgggacga tcccgcgctc gggccagaac aggatcgcgg ccagcaggac gagggacccg
    14641 aagatcgcca gcgctgccgc cgggtcgggg agggggctca tagaggtttt gcctccccaa
    14701 catcctgatt tagtacgaag aaaatgtcaa gcggcgatca ccggcggagg atgtcgggca
    14761 gcttcgccat gaacgcccgg acgtcgccga cctggcgtcg cgtggaaacg aactcgaagg
    14821 cggtggcgct gatcgagccg gtccgccgct cgagcgtcgc aatcagcgcg gccggtacga
    14881 tcgggcattc gtccagcacg cccagcaggc tcacccaggc cggcagatcg agcacggcaa
    14941 tcacgtcgag cgcgtctcgc gctcgccacg gggcaccggc ggcacggtgc ttcgccagct
    15001 gctgccggag gacgtggagc ccggcctgcg tctcgaggtc cgcgcaccgg agaacgccga
    15061 ggatggcgat caggcggtcg gcggcggaca ggctcatctc gtggagcgcg cgccagccgc
    15121 ggtggaacac cgagagcagg tcgtgcccga caaggaaccc ggcatgcaag ctcccccact
    15181 cctccagtcc caggttgcac gtggcgacga cggcatcgga ggcttcctgc ggagcaaagg
    15241 gcctggcgtg gatcgagcac cccgcgacga gcgcgttcgc gaggaacgcc agttcccggc
    15301 ttcgcgcctc gtaggcctcg tcgcctgcgt cgcgcacgcg ggccatcaac tcgcgcatgc
    15361 gctcgaggcg cgaggcttcg cccaccgttc cgggcagcag cgcccggggc cgctcgggca
    15421 ccagcccttc ttcggcaagc aggcggatta gtgcctcgat cggggcgtcg gtcccggcag
    15481 ccggcgcctc gggtgctttg cccaccgcat ccgggcggcc aggaagagcg gcgccgacct
    15541 ccccttgctg cccagcgccg tggagcgcgt agtcggcgaa cacgcgccga atcgacgcgg
    15601 cgtcggtggg gtcgccaaag ggcttccggg cgaggtcgag caccaggcgc gcatcggctg
    15661 gcgacaggta gccctgcccg gagcgccgcg cctctcggac cgaggcgagg tcgtggagca
    15721 gctgacccgg cgcggacaac aactcgtgaa gcccatccgc ctcggggacc gagttcgaca
    15781 gggcgcggca cccgcgcatc accgactcga agaacgggtg gtgcgcagcg tcgagcgcca
    15841 ggagcagaga cacgatcgcg tcccaggcgt cggtcctcct cgcgctcacg aggtagccgc
    15901 ccacctccgt cgcgaggttt cctggggtga gcggcgcatc acgggcggcg gggtcgaaaa
    15961 ccttcacgtg gcctgacagc ccggcggtta cgagccgctc gtcgatcgcc gagacgactc
    16021 gcgccgccac ctcgtcgccc atctcgacca acgccccgag ccattctcca aagcggccgg
    16081 gatcgaagcg ctcgtcaccc ccggccccga ccctgcgcca gagatcgacc tccagcaccg
    16141 acgagatctg ctccggcgtc gccgccgcga tcacctcgcc agacgactcg agcccccggt
    16201 actgaatgag ccgatgcagc gtctcggcgc gaaggcgcgg cacgacgcgc tcgagccgcg
    16261 ccgtctcgag caggcgcgcg agatctaccc gtctgcccat ggaaggggga ggatagccct
    16321 ttgtctgccg cacgaacagt cttgaactgc cctgcgggag gcgggacaat attggtgcag
    16381 caccgctcag tttgccgccc cggcgccctc cccgtacact tgactgctct gccaaaacct
    16441 gtgcgcgacc gccccggcct ccccggcccc gctgccggga gtgcggatga atgatatgtt
    16501 cgcaccacca ctgtcacgtg tcctgcgccc gccagggtcg cggctgaagc gctatgccat
    16561 cgcggtggcc ctggtgggcg cggccacgct cttcagggcg gccatcgagc cgctcgtcgg
    16621 caccacggtg ccgttcgggc tcttcgtgcc aaccgtcatc gtggcggcgt ggctcggcgg
    16681 cgcggaaggc gggctgctgg cgaccgccct tgcggcactt gccggcaagt tcctcttcat
    16741 cgagccccgg taccatgcga ccttcaccac tcgcgacata gcgatcgtcc tcctcgccgg
    16801 cgccgtcggc tcggggctcg ttctcctggt ctcggtctgg cgccggacgc agctccgcct
    16861 cctggacctg cacgacgaga tggcaggcat cctccgccag ctgccggccg gcgtcgtcgt
    16921 agcggatcgc tccgggagag tcgtgttcgt gaacacggag ctcgagcgcc tcctcggagg
    16981 gcctcttcgg ctcgggatgt tcgccgactt ccggcgtcag tacacgccgc tgcacgccga
    17041 cggtaccgcg atcgcgttgt cggagtcgcc cctgttgagg gcggcgaacg gcgaagaggt
    17101 gcccgaggag gaagccacga tcgtccgaag cgacggcgaa cggagaatcg tgcgggttcg
    17161 cggaacaccg gtgcggaggc acgacgggac ggttgccggg ggcgccgtga cggttgtcga
    17221 cgtcaccgag ctccgggaga ccgcgcgggt tgcgagagag cgggccgagg agctgggaca
    17281 ggcggatgcc aggctccgcg aacaggcgcg tgagctggag cgcgtcaacc gcctcaagga
    17341 cgaattcctg gccaccctca gccacgagtt gaggaccccg ctgacggcga tcgtcggatg
    17401 ggcggcaatg ctgcttcgag gcgggctgtc gcccgacgtg gtgcacaggg cccacgagac
    17461 catcgcccgg aacgccgact cgcagcgggc cctcatcgaa gacgtgctcg acgtgtcgag
    17521 gatcgtcacg gggaacctgc ggctggagaa ggggccggcc gacgtggagg gccccctgag
    17581 ggcggcggtg gaggcggtga agccccaggc ggaggaggcg ggcgtcacga tcacgaccgc
    17641 gctcacggat acgtcgacag cggtctgggg cgactccgtg cggctgcaac aggtgttcgt
    17701 gaacctcatg tcgaacgcga tcaagttcag cgggaggggc ggccgcatct gggtgtcgct
    17761 tcgccgggtg ggagcgaagg tggaagtgca ggtgcgggac aacgggatcg gcatctcggc
    17821 cgagcacctc ccccacatct tcgagcggtt caggcaagcc gacagctcga cgacccggcg
    17881 gcacaacggc ctcgggctgg ggcttgcgat cgtgaggcac atcacgggcc tgcacgacgg
    17941 gaccgtgaag gccgacagcc ccggcgaggg ccagggggca accttcacag tctgcctgcc
    18001 gtaccatccc ctggaggagg gtgcgcccac cgctacgagt ccgcctcgcc cgggggtctt
    18061 cgcagggccc gcagcgcccc cttctcccgg ttcgacaccc accctgcgcg gccgccgcgt
    18121 gctcgtcgtg gacgacgagg ccgacacgcg gcaactggtc gcgtcggtcc tgacagcatc
    18181 gggggcagaa gtcatttccg ccgcgtcggc cagggaagcg ctcgagcagc tccaggcggg
    18241 cgtgcccgac gcgatgctgg tcgacatctc catgccggag atggacgggt acgagttcgt
    18301 cagggcgatt cgtaacagcc gcagcggagc ggagcacgtc ccggcaatcg cgttcacggc
    18361 ccacgcgcgt gaggaagatc gccggctggc gctgaccagc ggcttccgga tgcacgtcgc
    18421 caagccggtc gagccgactg ccctggtgcg ggcgctggcg accgtcctgg aacggtagcc
    18481 gacacggagg acgggaccgg ctgtgccatt gcaaacgggt acgcgcctgg gtccgtacga
    18541 gatcgtcgag ctgcttgggg caggcggcat gggcgaggtg tatcgcgccc gggacccgcg
    18601 cctcgggcgg gatgtcgcga tcaaggtcat gactgccctg tcggatcggt cgcaggccct
    18661 ccggttccag caggaggccc gcgcggttgc cgccctctcg cacccgaacg tggtcgccat
    18721 cttcgacgtc ggaggggacg agcgcccgtt cctggtgacc gaactgctgc gcgggcaaac
    18781 gctgcgatcg ctcgtcgagc ggggccccgt cccgcttgcc gaggcggtcg agctctcgct
    18841 gcaggccgcg gcggggcttg gcgccgcgca cgagatggga atcgtccacc gcgatctgaa
    18901 gccggagaac ctgttcctga cggcggacgg tcgcctgaag atcctcgatt tcggcgtggc
    18961 gaagatggtg tcagccgacg gtggcgtcac caggacggca acgacgtccc ccggcatggt
    19021 cctgggcacg gtcgggtaca tgtcccccga acaggttcgc ggcctcgagg cggatgcacg
    19081 ctccgacatc ttcgcgctgg gggcggtcct gtacgagttg ctcaccgcgc ggcgtgcctt
    19141 cgacggcccc acgccggccg accgactggc cgcggtcctg cacgagtccc ccgccgccct
    19201 gcactttcca gcggcggtcc cggcgtcgct cgtgagtctc gtccagcgct gcttgcagaa
    19261 ggcccccgag gcgcgcttcc agtctgcccg ggagttcgca ctcgccgtgg aggggctcac
    19321 cctcgcccaa ccgaccgcgg ccgtgggagc cgggaatgcc gggcactcct cgattgccgt
    19381 gctgccgttc gcaaacctca gcgccgaccc cgacaacgag tacttcagcg acggcctggc
    19441 cgaggacctg accagtgccc tcgcccgcct cgcgccgctg cgcgtcgcgg cgtccacctc
    19501 ggcgttccgc ttccgacgcg gcggcaggga tctccgcgag gtgggacgcg agcttggcgt
    19561 cgagacggta ctcgaaggga gcgtgcggcg cgcgggcggt cgacttcgca tcacgacccg
    19621 gctggttgac gtggcgtccg ggtaccagct ctgggcggac cggtacgacc ggaaactcgc
    19681 ggatgtcttc gacgtgcagg acgagatcgt cgagtcgatc gtccaggcga tcgctccgac
    19741 tttggttccc gtggccaggg ccgccgccca tcgcggcacc cggaaccttg aggcctacga
    19801 gttgtacttg aaggggaggt acttctggaa ccagcggtcg ccggccgtcc tgcccgcggc
    19861 cctgcggagc ttcgaggagg ctgtcgcccg ggaccccgac tatgccctgc cctactgcgg
    19921 gctggccgac tgctacacca tcctgcacgc ctacggctgg atgcccccag cgatgtgccg
    19981 accgaaggcg gagcaatcgg tcgaaagggc gctggcgctc gagcccggcc tccccgaggc
    20041 ccgtctgtcg cgcgcgctct acgtgttcca cttcgaacgg cactggcggc aggcacggcg
    20101 ctcgtttgcc gaggcgctcg aggcacgccc gcgcatggcg atcgggcagg tgcagtacgc
    20161 cctgttcctc gccacggcgt acgagttcca ggaggcacgg gaacgaatcg acctggcgct
    20221 cgggctcgat cccgacgcgt cgatcatcca cttccttgcc gcgtcggcct gctgtctcac
    20281 cggcgacttc gaaggcgcgg agcggcacgc cacgcgggcg ctcgagctcc agcccgaatc
    20341 gctcggcgcc cggtggccgc tgacaatcgc gctgctcgca accggccgga cgtccgacgc
    20401 gatcgctgtc ggggaaaccg tcgtcgcgcg ctcacgcgcg cccgtgttcc tgggcgtgat
    20461 gtcgatggtg tacgggcggg cgggacggct tgccgacgcg cagcggctgg cacgggagct
    20521 cgacgagcgc cgcgcccgcg gcgagttcgt cgtccccttc gcggacctcg gcgtgcacct
    20581 cgggctcgag aaccgcgcag gcgtgcgcga cgccctggcg gcatgcgtgg acggcggcgc
    20641 cgcgcccatg gccgtcgcgg gcccgacacg tttcctcctg gacgggtaca ggagcgaccc
    20701 ggagatcgcg ctcctcctcg atcggctgca cgacgtcgag tagcgggctg cgagggttcg
    20761 gcattgcgct gcgcccgcgg acggcaggac aataccgtgg ggctgtcagc ttacacacgc
    20821 gccggggagg cgccctg
//
LOCUS       DAOPQX010000020        20456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3225_length_20456_cov_4.487182, whole genome shotgun sequence.
ACCESSION   DAOPQX010000020 DAOPQX010000000
VERSION     DAOPQX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 20456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20456
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3225_length_20456_cov_4.487182"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PKK95_02145"
     CDS             <1..863
                     /locus_tag="PKK95_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17035.1"
                     /translation="ARASRPEQRRPDATIVIAAGPRNDFLPPEIEALKRYLARGGKLL
                     LMLDPPNRADAPPLANLIALAHEWAIEVGNGVVVDVSGVGRLLGTSEEVPVAATYPSH
                     PIVEQFDLLTAFPLARPVSAVTAGVNSRFAQAFVETSPNSWAETDVKTMLTGGELKFD
                     EKSGDRKGPIAIAAAVSAAAESAEAQKADQKDPANGEKPETRVVVFGDSDFASNFALG
                     IQGNRDLFMNTLGWLSQQENLISIRPKETEDRRLTMTSGQQTRVLILVLVVLPLLVLG
                     SGVYTWWRRR"
     gene            864..2192
                     /locus_tag="PKK95_02150"
     CDS             864..2192
                     /locus_tag="PKK95_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOC17036.1"
                     /translation="MRSFKSVGVLALVLAALGAYIYFVERKKPSGPEAEARPKVFDVE
                     ADKIVEVMVRSGADRTVVRKVDGEWRVVEPIEAKADQAEVSGITSSLASLENVRVVEE
                     APSDVAQFGLDKPSIEVGFRTEGEKAMRHLRLGDKTATQGELYASRGGKQVFLVPAYL
                     DTSFMKSTFDLRDKSILAFARDKVDRVAIEGGTAPKTLVKSGSDWTLTAPLQAPADYG
                     AVESLVGRLETGRMSAIIEQQAADLKRFGLDKPALSVTIGTGSSQATLQVGSKADTGA
                     YYARDASRPLVFTLESGVVDELQKPAGDLRNKDVFAFRPFNATALELTRGDEVLSIEK
                     AKAKDGGEVWRRVKPSAGDLDRAKADDVLARLSNLRAEAFEAEGTAAAKALASPTLVV
                     RARYGEGKEETVRFARAGSDVYAGVPSQPGALKVPSGDFDEAVKAIDALK"
     gene            2226..3746
                     /gene="dacB"
                     /locus_tag="PKK95_02155"
     CDS             2226..3746
                     /gene="dacB"
                     /locus_tag="PKK95_02155"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOC17037.1"
                     /translation="MQHTLRRAGAALLLALAVSACGAKAPPAAAPVSPAVTAPAVPLS
                     SLARELDRIFAAPDFDRMHWGVLVRSLETGQVLYERQAGKLLMPASNMKIVTLAAAAE
                     RLGWDHTWTTSVTPAGRVDGGTLKGDLVVAGTGDPTINGRSGEATAVFQKWADLLLAA
                     GLRRIDGRIVGDASAFDDEELGAGWAWDDMAFDYSAPVSALQFNEDIARLVVRPGPGE
                     GSPATIELTPAESGLVVDNRVTTGPGDRVALSLRRLPGSERLTVSGSIGQLAKESARL
                     VAVDSPSAYFARALRRVLVERGIEVTGPAVDVRALDVKPVLDPVEPFFVHHSPPLSDA
                     ARVLMKVSQNLYAETFLKTIGAVAGEGGNAESGRKEVARVLQSWGIPPEEYVLADGSG
                     LSRYNYLTPHMLVTILERIYRDPRHRDPFIAALPVGGEDGGTIARRFKGTRAAGNVKA
                     KTGSLANVRALSGYVTSLDGEPLVFSIIANNFTVPQATIDAHIDRAVEHLANMTRR"
     gene            complement(3756..4946)
                     /locus_tag="PKK95_02160"
     CDS             complement(3756..4946)
                     /locus_tag="PKK95_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOC17038.1"
                     /translation="MSDRLYYEDSAQLEFDASVVTVTGVEGRPGVVLDRSAFYPTSGG
                     QPFDTGWLGDARVLEVIEDEAGRVVHVLDKPLEAGRNVHGRVDAERRLDHMQQHTGQH
                     ILSAALDRLSRARTVGFHLGADVSTVDLDVPLSPEALAAAEAEANRIVWEDRPVAIRT
                     MSEADAATLPLRKEPERTGALRIIEVEGYDLSACGGTHVARTGSIGLIATLSGERLRG
                     GTRVEFVCGGRALRYLRLYRDAVAGCIRSVSVAPADLPAAVARMQAEAKEQGKFIRTL
                     QERLATYEAAAVAATADFLPDGTRLLVRVVPGADAGVLKATASAVCAQPGFRVALFSS
                     STPRLALVARSKDVKVDASAALRALFAEFGGKGGGRPDLSQGGGLDGPEERLLARARE
                     ILSE"
     gene            complement(4981..5667)
                     /gene="queC"
                     /locus_tag="PKK95_02165"
     CDS             complement(4981..5667)
                     /gene="queC"
                     /locus_tag="PKK95_02165"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOC17039.1"
                     /translation="MTKAQPAVVLLSGGLDSATAAAVARREGFEVHALTVSYGQRHER
                     EVEAARRVARSLGAARHVELGVDLAAFGGSALTGSGQVPKDRPIELGVIPSTYVPARN
                     LVLLSLALAWAEAEGARDLFIGVNALDYSGYPDCRPEFVESFERTARLATKAGVEGAS
                     FVVHAPLIRMTKAEIIRLGVGLGVDYSLTWSCYDPAPGGAPCGRCDSCQLRARGFAEA
                     GLSDPLLAAR"
     gene            5789..6043
                     /locus_tag="PKK95_02170"
     CDS             5789..6043
                     /locus_tag="PKK95_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOC17040.1"
                     /translation="MDFKDINREPPSRAFLERHVVEGRELEFLGKRSPILKDRPLPTS
                     KREAIDLMLEQPNVIKRPVLVLGDEVVFGFDEARYRQLFG"
     gene            6262..6909
                     /locus_tag="PKK95_02175"
                     /pseudo
     CDS             6262..6909
                     /locus_tag="PKK95_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            complement(6944..7744)
                     /gene="nth"
                     /locus_tag="PKK95_02180"
     CDS             complement(6944..7744)
                     /gene="nth"
                     /locus_tag="PKK95_02180"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOC17041.1"
                     /translation="MKPASPVPLARPPVAAVMTRLARAIEQHDLPAVEKIAQSRLDKA
                     FQVLIGTLLSARTQDATTDAASARLFAAAPTPERMAALGAGEIEHLIYPVSFYRNKAR
                     HVKATALAILERFGGRVPATMEELLTLPGVGRKTANLVLILAFGSRENICVDTHVHRI
                     ANRLGWVRTRTPEETEQALYRVAARRWWPDINLYLVVWGQNVCRPVRPRCAACVVSRL
                     CPRVGVSQPASGAEPSVRRREAQGTLRPAPLGTPPRSARRASASTRSR"
     gene            complement(7777..8748)
                     /locus_tag="PKK95_02185"
     CDS             complement(7777..8748)
                     /locus_tag="PKK95_02185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOC17042.1"
                     /translation="MKPRVLLTRRVPATVYRRLEEACEVDLHGEAAAIPGDELRRRVA
                     DKDALLCLLTDRVDREVLEAAPRLKIVANIAVGYDNVDVASAAARGIVVTNTPEVLTE
                     ATAELTWALILAATRRIAEGDRAVRAGRWGGWALDYMLGMQLSGKQLGVIGLGRIGTA
                     VASRAPAFGMSVAAASLPGQPVSPSGGVPLPPIVTVPLDELLRTSDVVTLHVPLTPDT
                     RHLIDRPRLARMKRSAYLVNTSRGPVVDEEALAWALGERLIAGAALDVYEREPLVHPA
                     LLPLDNVVLAPHLGSATTETRTAMADLAVSNVLEVLAGRPPLTPVHP"
     gene            complement(8745..9014)
                     /locus_tag="PKK95_02190"
     CDS             complement(8745..9014)
                     /locus_tag="PKK95_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17043.1"
                     /translation="MLIRILLLIILLVLLGRAIRSLLASAAGAPLADGRRKPAERGVR
                     MVRDPVCGTFLPPTNALAEPARDGSVLYFCSEACRQEYAAQEKRR"
     gene            complement(9024..9662)
                     /locus_tag="PKK95_02195"
     CDS             complement(9024..9662)
                     /locus_tag="PKK95_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC17044.1"
                     /translation="MKPESIVLAIAGAFFGLIVGWIIGTQQAGPRALAGVPPAAAVQP
                     AGGAAPAAQPQAPPLDEARVQVLRAAAEKNPSDAQSRVQLGNAYFDAERYGEAVTWYE
                     AALGIDPKDPNVSTDLGVAYYYLNQPDRALEQFERSLAVDPAHTKTLLNQGIVRAFGR
                     QDLKGAAESWQRLIAVAPTSPEADAARRALQGLQSAHPDLAGTAPAAQPPGK"
     gene            complement(9694..10413)
                     /locus_tag="PKK95_02200"
     CDS             complement(9694..10413)
                     /locus_tag="PKK95_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC17045.1"
                     /translation="MTGHRLVVPALLAWLALSPWAASASTQQHVHFVTGDGVSIAATL
                     YLPSRPGPAVVLIHMQTRSRQDWEAVGPRLADAGLVALAIDLRGHGASDPRPTGPSAK
                     DGGIVADLRAARNYLATRREVTPGKVGLLGASIGANLAVVAAAADPTVRSIALLSPGL
                     DYQGLRTQANLNRYGDRPALLVGSREDAYVMITLRRLSSEGTGVREQLVLDGAGHGTT
                     MLARQAGLVGVLVDWFKRTLL"
     gene            complement(10410..11117)
                     /locus_tag="PKK95_02205"
     CDS             complement(10410..11117)
                     /locus_tag="PKK95_02205"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOC17046.1"
                     /translation="MRLAVNIDHIATIREARKAREPEPIAAALLAELAGAQGITVHLR
                     GDRRHIKDRDVELLRQMVATKLNIEMAATEEMTGIALRVRPDQVTLVPEQPHEITTQG
                     GLDVVANRAAVIAATRRLREAGIRVSIFIDPDEAQVAASKEVGADAVEINTGGYADAS
                     PSSRAARLAEVRRSAEAASAGGLEVLAGHGLTYFNIGPVASIRTIEELNIGHSIISRA
                     SLVGLERAVREMVELLR"
     gene            complement(11216..13318)
                     /locus_tag="PKK95_02210"
     CDS             complement(11216..13318)
                     /locus_tag="PKK95_02210"
                     /inference="COORDINATES: protein motif:HMM:NF006194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HOC17047.1"
                     /translation="MKSLWSETEARRFVDLHGPAWGEALALRTYSARLLGAEPGLVLH
                     GGGNTSVKAETSDVFGDRHEALFVKASGADLAAIGPDGHVCLALGPLRRLRALDRLGD
                     DEMVWELRRQLLRAGAGAPSIEALVHAFLPHRFIDHTHADAVLALTNRAGGEALAGEV
                     LGDGVIVMPYAEPGFELAKAAADAFEAAPAARAMVWARHGLVTWGRDARESYEATIEL
                     VSRAEACLTGRARARVVSADQAARTREGIADAAPVLRGLLAGPRDEATGRRRRVVVRA
                     LATADVLELLESPGMREAALAGPLTSDHLIRTGPWPAWATLEAAGGGPAIADVFRSAI
                     GAYAARYRDYLQRHASMMPRELDAFEALPRVVLAPGLGAFCAGRDLRDAEIARDITAH
                     TLAVKASIAGSGTYEGLPERDLFHMEYRTLQHAKLAGREGGALTGEVAIVTGAAGAIG
                     SGICRALLEAGCLVAATDLPGPGLESLVTELSEVFGPAIIGVPMDVTDAGAVGEAWRR
                     VVEEWGGVDLVVVNAGIAHVSSLATMDLSAFRRLERVNVEGTLLVLGEAARLFERQGS
                     GGDVVLVSTKNVFAPGAKFGAYSATKAASHQLARIASLELAGLDVRVNMVAPDAVFSD
                     GSRKSGLWAEVGPDRMRARGLDEAGLEAYYRSRNLLKAPVTARHVANAVLFFATRQTP
                     TTGATIPVDGGLPDATPR"
     gene            complement(13466..14185)
                     /locus_tag="PKK95_02215"
     CDS             complement(13466..14185)
                     /locus_tag="PKK95_02215"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOC17048.1"
                     /translation="MTGTPAAALSAACPTISVGLLTSDLLSLGSELKVLEDAGVKVVH
                     VDVMDGCFTPMMTVGPPFVKALRRTPLLKDVHLMIRDPLEKVREYVAAGADIVTVHVE
                     SSIHVHRVLQSLGQMPHAANPSRRVVRGIALNPGTPVSSIEPLLPEAEMVVLLAVNPG
                     WGGQAFIPATLDRVRQVRDLAAGSGREGLIVCVDGGVTRANAAEIAKAGADLLVTGSA
                     VFDGRHAAENARAMLAAVGAQ"
     gene            complement(14182..15084)
                     /gene="lsrF"
                     /locus_tag="PKK95_02220"
     CDS             complement(14182..15084)
                     /gene="lsrF"
                     /locus_tag="PKK95_02220"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HOC17049.1"
                     /translation="MADLDNLKDTKDYHLDVPARNEPFFLKGAGALDWGMQNRLARIF
                     NPRSGRTVMLAIDHGYFLGPTSGLERIDVNIVPLLPYADALMLTRGILRTTIPPSFGR
                     GVVMRASGGPSIRKELSNELIAVDIEDGLRMNVAAMAIQVYIGGEYETQTVNNMTRMV
                     DMANRYGIPTLGVTAVGKDMVRDAQYFRLATRICAELGAQFVKTYYVADGFETVTASC
                     PVPIVMAGGKKMPELEALTMAFRAVDEGAAGVDMGRNIFQSAAPTAMIQAVQKVVHEL
                     MKPEHAYELYQELAAQAKRDRAAV"
     gene            complement(15087..16448)
                     /gene="glmM"
                     /locus_tag="PKK95_02225"
     CDS             complement(15087..16448)
                     /gene="glmM"
                     /locus_tag="PKK95_02225"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOC17050.1"
                     /translation="MAHRLFGTDGVRGRAGAYPLDSETIRRLGAALVRALPADDGRHR
                     LLVGRDTRESGSWIVREFAHGVRSAGGSIVSAGVISTPAVAYLAREQAFDAGVVISAS
                     HNPFEDNGIKVFSGAGEKFTEQLERAVEAVVADRSWQVPAGEPAPLEDLDLTTLYLEH
                     ARIALPSAGRLAGLRLAVDCANGATSAIAPRLFRELGFDLVVLGCEPDGRNINLECGS
                     TKPAALARTVVAQGCRMGVAFDGDGDRAIFADASGRIVDGDGVLLLAARHLQSAGRLR
                     GNAIVATIMSNIGLEIALRESGIALVRCPVGDKHVMEELIARNLSLGGEQSGHVIFSD
                     FLFTGDGLVTALNVLQVMAETGRELGDLAGELVTYPQVLVNVRVKEKRELQGIPEIAA
                     VMDDVERRLGASGRLVVRYSGTEPLLRVMIEGQDQHQITAWATEIADAARRHLGEPAE
                     GAA"
     gene            complement(16472..17389)
                     /locus_tag="PKK95_02230"
     CDS             complement(16472..17389)
                     /locus_tag="PKK95_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOC17051.1"
                     /translation="MAYYHPFRHLGLKFISVAIAAALWFVVAGEETVERSVRAPLELQ
                     NAPESLVLVENPPTAVDVRVRGAGGLLRQLSAAEVVAVLDLSSARPGRRLFHLTPQNV
                     RAPFGVEVTQVSPGTISLVFERSGSRTVSVVPAIEGEPAEGYKVGAITSDPATVVVVG
                     PESALRDVTEAITDPVSIEGARRVVRESATVGVADPRVRLLKPVNARITVDILPLPVQ
                     RAVLNVPVRLHNAGRGLSAQSVPPVVTVLTKGRKEVLDELRTDAVGAFVDLAGLGPGR
                     YNLPVRVAPSQDYEVLRIEPARVRVRVVR"
     gene            complement(17389..18192)
                     /gene="cdaA"
                     /locus_tag="PKK95_02235"
     CDS             complement(17389..18192)
                     /gene="cdaA"
                     /locus_tag="PKK95_02235"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOC17052.1"
                     /translation="MDALLGILQRLGWWDVLDILIVSVAIYEILKLIRGTRAVQMGLG
                     AGFLLALFYLSRLGHLTTVNWLIRNMIGYVVFALIVLFQADIRRTLAHFGRTPFAGYF
                     GRGEGGDDVIEEIAVAAGVLASQRVGAIIAIERDIGLRNYIEGGIPLDATVTNDLLVT
                     IFKPGTPLHDGAVIVQGNRVAAAACFLPLTINPVVSKDFGTRHRAAIGLTEETDALAV
                     IVSEETGRISVALEGRIEVGVAADILRTRLRELLAAREARPRERRGLYD"
     gene            complement(18223..19098)
                     /gene="folP"
                     /locus_tag="PKK95_02240"
     CDS             complement(18223..19098)
                     /gene="folP"
                     /locus_tag="PKK95_02240"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOC17053.1"
                     /translation="MFPLTSYVVPVAGGDPLVLGDRTLVMAIINVTPDSFAEASPSVD
                     PARALDLAQAAAEHGADILDLGAESTRPGSAVVPEDEELRRLLPALRAVSARVRLPIS
                     VDTRKAGVARAALDEGAGLVNDVSGLMYDPALGGVVARAGAGLVLMHTRGTPDRMYRE
                     AAYGDLVAEVRDELGQAMERAARAGVPREAIVLDPGIGFAKHAGQSYEVLAGLRELAA
                     LGRPLLVGPSRKSFLQAAAGERPAAERDWGTAAAVTAAILGGAHVVRVHAVAEMVQVA
                     RVADAIRLAGRPGAR"
     gene            complement(19222..>20456)
                     /locus_tag="PKK95_02245"
     CDS             complement(19222..>20456)
                     /locus_tag="PKK95_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC17054.1"
                     /translation="VGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFESNEGVILVAATNRPDVLDPALLRPGRFDRRIVVNRPDVKGREG
                     ILAVHTRKIPLSDDVNIGVLARATAGFCGADLANLVNEAALYAARYNQKVVRMMDFEF
                     AKDKVLMGSERRSMIISDAEKQVTAVHEAGHALLTVLLPGADPIHKVTIIPRGMALGL
                     TQQLPVDEKHNYSKPYLDDQISILLGGRVAEEILTGNVTTGAGNDLERVTDLARKMVC
                     EWGMSPAMGPLTFGKKEEQIFLGREIAQHQDYSEDTAIRIDQEVKAIVTSNYDRAHAM
                     LAENRQGLVRIADALLLHEVLDGEQVKRLAAGESIQDVVSRPSVPASDEDEARARQRD
                     RGAIVPPLQPIQKPLPQE"
BASE COUNT         3052 a         7291 c         7263 g         2850 t
ORIGIN      
        1 tcgcccgcgc ctcccggccc gaacagcgtc gccccgacgc caccatcgtc atcgcggcgg
       61 gaccgcgcaa cgacttcctg ccgcccgaga tcgaggcgct gaagcgctac ctcgcgcgcg
      121 gcgggaaact cctgctgatg ctcgacccgc ccaaccgcgc cgacgcccca ccgctggcca
      181 acctgatcgc cctcgcgcac gagtgggcga tcgaggtcgg caacggcgtc gtcgtggacg
      241 tcagcggcgt cggccgcctg cttggcacca gcgaggaggt cccggtggcg gcgacctacc
      301 cgtctcaccc gatcgtcgag cagttcgacc tcctcaccgc cttcccgctg gcgcggcccg
      361 tgtcggccgt gaccgccggc gtcaacagcc gcttcgcgca ggccttcgtc gagaccagcc
      421 cgaacagctg ggccgagacc gacgtgaaga cgatgctcac cggcggtgag ctgaagttcg
      481 acgagaagtc gggcgaccgg aaggggccga tcgccatcgc ggcggcggtg tcggcggcgg
      541 ccgagagcgc ggaggcgcag aaggcggacc agaaggaccc ggccaacggc gagaagcccg
      601 agacgcgggt ggtcgtcttc ggcgactccg acttcgcgtc gaacttcgcg ctcggcatcc
      661 aggggaaccg cgacctgttc atgaacacac tgggctggct ctcgcagcag gagaacctca
      721 tctcgattcg cccgaaggag accgaggatc ggcgcctgac gatgacctcg ggacagcaga
      781 cgcgggtgct gatcctggtg ctggtggtgc tcccgctgct cgtgctcggc tcgggcgtct
      841 acacctggtg gcggaggcgg tagatgcgca gcttcaagtc ggtgggcgtc ctggccctcg
      901 tgctcgcggc cctgggcgcc tacatctact tcgtcgagcg gaagaagccc agcgggccgg
      961 aagccgaggc gaggccgaag gtcttcgacg tcgaggcgga caagatcgtc gaggtgatgg
     1021 tccgctcggg cgcggaccgg accgtcgtga ggaaggtgga cggcgagtgg cgcgtcgtcg
     1081 agccgatcga ggccaaggcc gaccaggccg aggtgtcggg catcacgagc agcctcgcca
     1141 gcctggagaa cgtgcgggtg gtcgaagagg ctccgtcgga tgtcgcgcag ttcggcctcg
     1201 acaagccatc gatcgaagtc ggcttccgca ccgagggcga gaaggcgatg cgccatctgc
     1261 gcctgggcga caagacggcg acgcagggcg aattgtacgc ctcgcgcggc ggcaagcagg
     1321 tcttcctggt gcccgcgtac ctcgacacgt cgttcatgaa gagcaccttc gacctgcgcg
     1381 acaagagcat cctcgccttc gcgcgcgaca aggtggaccg ggtggcgatc gagggcggca
     1441 ccgcgcccaa gacgctggtg aagtcggggt ccgactggac gctgacggcc ccgctgcagg
     1501 cgccggccga ctacggcgcc gtcgaatcgc tcgtcggacg gctcgagacc ggccgcatga
     1561 gcgcaatcat cgagcagcag gcggccgatc tgaagcggtt cggcctcgac aagccggccc
     1621 tgtcggtcac catcgggacc ggcagttccc aggccaccct gcaagtcggt tcgaaggccg
     1681 acacgggcgc gtactacgcc cgcgacgcct cgagaccgct ggtcttcacg ctcgaatcgg
     1741 gcgtggtcga cgaactgcag aagccggccg gcgacctgcg gaacaaggac gtgttcgcct
     1801 tcaggccgtt caacgcgacc gccctcgagc tgacgcgcgg cgacgaggtg ctgtccatcg
     1861 agaaggccaa ggcgaaggac ggcggcgaag tgtggcggcg cgtgaagccc tcggccggtg
     1921 acctcgatcg cgccaaggcc gacgacgtgc tcgcgcggct gtcgaacctg cgagccgaag
     1981 cgttcgaggc ggagggcacc gcggcggcga aggcgcttgc ctcgccgacg ctcgtcgtgc
     2041 gcgcccgcta cggggagggc aaggaggaga cggtgcggtt cgcgcgggcc ggctccgacg
     2101 tctacgccgg cgtgccgagc cagcccggcg ccctgaaggt gccctccggc gacttcgacg
     2161 aggcagtgaa agccatcgac gcgctgaagt agcgcagggc gttagccctg cgggacaagc
     2221 tcctcatgca acacaccctt cgccgagccg gcgcggcgct gctgctggcg ctcgccgtct
     2281 cggcgtgcgg cgcgaaggcc ccacccgcgg ccgcaccggt ctcccccgcg gttaccgctc
     2341 ccgcggtgcc gctctcgtca ctcgcgcgcg agctggatcg gatcttcgcc gcgcccgact
     2401 tcgaccgcat gcactggggc gttctcgtgc gctcgctcga gacgggccag gttctctacg
     2461 agcggcaggc gggcaagctg ctgatgccgg cctccaacat gaagatcgtg acgctcgcgg
     2521 ccgcagccga gcgcctgggc tgggaccaca cgtggaccac gtctgtgacc cccgccgggc
     2581 gcgtcgacgg cggcaccctg aagggcgacc tcgtggtcgc gggcaccggc gacccgacga
     2641 tcaacggccg cagcggcgaa gcgacggccg tcttccagaa gtgggccgac ttgctgctgg
     2701 ccgccggcct ccgtcgcatc gacggccgca tcgtcggtga cgcctcggcg ttcgacgacg
     2761 aggaactggg agcggggtgg gcctgggacg acatggcgtt cgattactcc gccccggtca
     2821 gtgcgctcca gttcaacgag gatatcgcgc gcctcgtcgt caggccgggc ccgggcgagg
     2881 ggagcccggc gaccatcgag ctcaccccgg ccgagagtgg cctcgtcgtg gacaaccggg
     2941 tgacgacggg gccgggcgac cgggtggcgc tgtcgctgcg gcggctcccg ggttcggagc
     3001 gcctcaccgt gtcgggcagc atcgggcagc tggccaagga gtcggcgcgg ctggtggcgg
     3061 tggacagccc gagcgcctac ttcgcccgcg cgctgcggcg ggtgctggtg gagcggggaa
     3121 tcgaggtgac gggccccgcg gtggacgtgc gcgcgctcga cgtgaagccg gtgctcgacc
     3181 cggtcgagcc gttcttcgtc caccactcgc cgccgctgtc ggacgcggcc cgcgtgctga
     3241 tgaaggtgag ccagaacctg tacgccgaga ccttcctcaa gacgatcgga gcggtcgcgg
     3301 gtgagggagg caacgccgag tcgggccgga aggaggtggc gcgggtcctg cagagctggg
     3361 gtatcccgcc ggaggagtac gtccttgccg acggatcggg gctctcacgc tacaactacc
     3421 tgacgccgca catgctggtg acgatcctgg aacggatcta ccgcgacccg cgccaccgcg
     3481 acccgttcat cgcggccctg ccggtggggg gcgaggacgg cggcaccatc gcgaggcggt
     3541 tcaagggcac gcgggcggcg ggcaacgtga aggcgaagac gggatcgctc gccaacgtcc
     3601 gcgcgctctc gggctacgtc acaagcctcg acggcgagcc gctggtcttc tcgatcatcg
     3661 ccaacaattt cacggtcccc caggccacga tcgacgcaca catcgaccgg gccgtcgagc
     3721 acctggcgaa catgacgcgg cggtgaaggg cggggttatt ccgacaggat ctcccgcgca
     3781 cgcgccaaca ggcgctcctc gggcccatcc agcccgccgc cctgcgagag atcgggccgt
     3841 ccaccgccct tgccgccgaa ctcggcgaac agggcccgca gggcggcaga ggcatccacc
     3901 ttgacgtcct tggaccgcgc gaccagcgcc aggcgcggcg tggacgacga gaagagggcg
     3961 acgcggaagc cgggctgcgc gcagacagcc gaggccgtcg ccttcagcac gccggcatcc
     4021 gccccgggaa ccacgcgcac gaggaggcgg gttccgtcgg gaaggaagtc ggcggttgcc
     4081 gcaaccgcgg cagcctcgta cgtggccagg cgctcttgca gggtcctgat gaacttcccc
     4141 tgctccttgg cctcggcctg catgcgggcc acggccgcgg gcaggtcggc cggggccacc
     4201 gagacggagc ggatgcatcc cgccaccgcg tcacggtaga ggcgcaggta gcgcagcgct
     4261 cgaccgccgc acacgaactc gacgcgtgtc ccgccccgca gccgctcacc cgacagggta
     4321 gcgatcagtc cgatgctgcc ggtgcgcgcg acatgcgtgc cgccgcaggc cgacaggtca
     4381 tatccctcga cctcgatgat ccgcagcgcg cccgtccgct cgggctcctt gcgcaagggc
     4441 agggtggccg cgtccgcttc cgacatcgtg cgaatcgcga cgggccggtc ctcccagacg
     4501 atccggttgg cctctgcctc cgcggccgcg agcgcctcgg gcgagagcgg cacgtcgagg
     4561 tccacggtcg aaacgtcggc tcccaggtga aacccgacgg tgcgggcgcg actcaggcga
     4621 tcgagcgcgg cggagaggat gtgctgcccg gtgtgctgct gcatgtgatc gaggcgccgc
     4681 tcggcatcga cacggccgtg aacgttccgg ccagcctcga ggggcttgtc gaggacgtgc
     4741 acgacccggc cggcctcgtc ctcgatcacc tccagcaccc gggcgtctcc aagccagccg
     4801 gtgtcgaacg gctgcccccc ggacgtcgga tagaaggcgc tgcgatcgag caccacgccg
     4861 gggcgtccct cgacgccggt cacggtgacg acggacgcgt cgaactcgag ctgcgcggaa
     4921 tcctcgtagt aaaggcggtc ggacacgggc cctcctcgat accgaatccc ggcgatccgc
     4981 tcagcgcgcc gcaagcaggg ggtcgctgag acccgcctcg gcgaatcccc gggcgcgcag
     5041 ctggcaactg tcgcagcggc cacagggggc gccgcccggc gcagggtcgt agcagctcca
     5101 ggtcaacgag taatcgaccc cgagccccac gccgaggcgg atgatctccg ccttcgtcat
     5161 gcggatgagc ggcgcgtgga ccacgaacga cgctccctcg acgccggcct tcgtcgccag
     5221 gcgcgcggtg cgctcgaagc tctcgacgaa ctcgggccgg cagtcggggt agccggagta
     5281 gtcgagggcg ttcaccccga tgaagagatc gcgggctccc tccgcctcgg cccaggcgag
     5341 cgccagcgaa agcagcacca ggttgcgtgc cggcacgtag gtggacggga tgacgcccag
     5401 ttcgatcggg cggtccttgg gaacctgccc gctcccggtc agcgccgacc ctccgaacgc
     5461 cgcgagatcc acgcccagct cgacgtggcg agccgccccg agcgagcgcg ccacccgccg
     5521 ggccgcctcc acctctcgct cgtgccgctg accgtagctg accgtgaggg cgtgcacctc
     5581 gaagccctcg cggcgcgcca ccgccgccgc ggtggccgag tcgagcccgc cgctgaggag
     5641 caccaccgcc ggttgcgcct tcgtcatccc gccattgtag ttagaatggg cctcggaggt
     5701 gcgtgtgcgg aaaccgacct tctactgcaa gcccacctga acgacctgcc ggaaggccag
     5761 gagttacctg gaccagctgg gcgtcgtcgt ggacttcaag gacatcaacc gtgaaccgcc
     5821 gtcacgcgcc ttcctcgagc gtcacgtggt ggagggccgc gagctggagt tcctcggcaa
     5881 gcgcagtccg atcctcaagg accggccctt gcccacctcc aagcgcgagg caatcgacct
     5941 gatgctcgag cagccgaacg tgatcaagcg cccggtcctc gtcctgggcg acgaggtcgt
     6001 cttcggcttc gacgaggcgc ggtaccgcca gctcttcggc tagcggtcgg tgcgaagggg
     6061 attcggatcg ggcttgggat tcgtcgattg ggattgggat tgggattggg attgggattc
     6121 atcagaatcg gcacctccgg ctggagctat cccgcgggtc aaggcacctg gaacggactg
     6181 ttctaccccc cgcgcgggaa gggcggcttc aaggcggccg acgagctgcg ctactacgcg
     6241 tcgcggttcg acaccgtgga ggtgaactcg acgttctacc ggacgccggt gccggcgacc
     6301 acgcggcggt gggccgcaca aacacccgcg agcttcgagt tctccatcaa gctgtttcag
     6361 aagttcacgc accccggcat ggtcgggcgc ggcgcccccg cggcccgcag gccggccacc
     6421 ggaccgcgtc cgctccccga gccgacgccg gccgacctgg acgagttcct tgcggcgctc
     6481 gatccgctcg ccggaatcgg gaagctgggg gcgctgctcg tgcagttccc ggccggcttc
     6541 cgggcggatc ccgagtcgcg cgactacctg gcgcgcctcc tcgagtggtt ccgcgactac
     6601 ccgctggccg tcgagctccg ccaccggtcg tggagcgacg agcagcgcga gaccgacacg
     6661 ctgctcgggg cggcgcgcgc ggccctggtg cagatcgacg agccgaagtt ccgcttctcg
     6721 atccggcagg atcgcgagcc gaacgtccgg tcgcactatt acgtgcggct gcacggccgc
     6781 aacgcggcgc agtggtggac gcacgactcg cccgacgatc gctacaacta cctctactcg
     6841 ccggcggaac tccgcccgat cgccgagacg atcaagcgcg tcggccgaag cgtggacaag
     6901 acgcacctct acctgaacaa ccactactcg gcgaaggcgg tcgtcaacgc gaccgtgttg
     6961 aagcagatgc tcgacgtgcc gatcgagggg gagtacccag aggagctggt cgaagcgtac
     7021 cctgagcttc gcggcgtcgt acggacggct ccgcaccgga cgctggttga ctgacgccga
     7081 cgcgcgggca gagacggctc accacgcagg ccgcgcacct ggggcgcacc gggcggcaga
     7141 cattctgccc ccagacgacg aggtagaggt tgatgtccgg ccaccagcgt cgcgccgcca
     7201 cccggtagag cgcctgctcg gtttcctcgg gcgtcctcgt tcgaacccag ccgagacggt
     7261 tggcgatccg gtggacgtgg gtatcgacgc agatgttctc gcggctgccg aaggccagga
     7321 tcaggaccag gttcgccgtc ttccgtccca cgcccggcag cgtcagcagc tcctccatcg
     7381 tcgctgggac ccgcccgccg aagcgctcga ggatcgccag cgccgtcgcc ttcacgtggc
     7441 gcgccttgtt gcggtagaag ctcaccgggt agatcaggtg ctcgatttcg ccggcgccga
     7501 gggccgccat gcgctcgggc gtcggggcgg ccgcaaacag gcgcgccgag gccgcgtcgg
     7561 tggtcgcgtc ctgcgttcgc gccgacagca gcgtcccgat cagcacctgg aacgccttgt
     7621 cgaggcgcga ctgcgcgatc ttctcgaccg ccggaaggtc gtgctgctcg atcgcgcggg
     7681 cgagccgcgt catcacggcg gcaaccgggg ggcgggcaag gggaacaggt gaagccggtt
     7741 tcatgggtgg aacgccggca gggcgaaagc cctgtcctac gggtgaacgg gtgtcagggg
     7801 cgggcgcccg gccaggacct cgaggacatt gctgacggcg aggtcagcca tcgcggtccg
     7861 cgtctcggtc gtcgcgctgc caaggtgcgg tgccagcacc acgttgtcga gaggcagcag
     7921 cgccgggtgc accagcggct cgcgctcgta cacatcgagg gccgcgcccg cgatgagccg
     7981 ttcgccgagc gcccacgcga gcgcctcctc gtccacgacc ggccccctgg aggtattgac
     8041 caggtacgcg gaacgcttca tcctcgcaag ccgtggccga tcgatcaggt gacgagtgtc
     8101 gggcgtgagc ggaacgtgga gcgtgaccac gtcggaagtc cgcagcagct cgtcgagcgg
     8161 cacggtgacg ataggcggca ggggcacccc gccggacggc gagaccggct ggcccggtag
     8221 cgaagcggcg gcgacgctca tgccgaaggc gggcgcccgc gaagccaccg ccgtgccgat
     8281 gcgcccaaga ccgatcaccc ccagctgctt cccgctcagc tgcatcccca gcatgtagtc
     8341 gagcgcccaa ccgccccacc gccccgcccg caccgcgcgg tcgccctcgg cgatgcgccg
     8401 ggtcgccgcg aggatcagcg cccaggtgag ctcggcggtg gcctcggtca acacctccgg
     8461 ggtgttggtg acgacgatgc cgcgcgcggc ggccgacgcg acgtccacgt tgtcgtagcc
     8521 gacggcgatg ttggcgacga tcttcagccg tggagccgct tcgagcacct cgcgatcgac
     8581 gcggtccgtc agcaggcaca gtagggcgtc cttgtcggcc acgcggcgcc tcaactcgtc
     8641 gccagggatg gccgcggctt cgccgtggag atcgacctcg caggcttcct cgaggcgccg
     8701 gtagacggtg gccggcacgc gtcgagtcag caggaccctc ggcttcaacg gcgcttctcc
     8761 tgcgcggcgt attcctggcg gcaggcctcc gagcagaaat agaggaccga gccgtcgcgc
     8821 gccggctcgg cgagggcatt cgtgggcggc aggaacgtgc cgcaaacggg atcccgaacc
     8881 atgcggacgc cgcgctcggc cggcttcctc cgtccgtccg ccaacggcgc ccccgcggcg
     8941 ctggcgagca gcgagcggat ggcccgcccc agcagcacca ggaggatgat cagcaggagg
     9001 atgcggatga gcatcggccg ggcctacttc ccggggggct gcgccgcggg cgccgttccg
     9061 gcgagatcgg ggtgcgccga ctgcaggccc tggagcgcgc ggcgagccgc gtcggcctcc
     9121 gggctcgtgg gcgcgaccgc gatcaggcgc tgccacgact cggccgctcc cttcaggtcc
     9181 tgccggccga acgcgcggac gatcccctgg ttcaacagcg tcttggtgtg cgccggatcg
     9241 acggccagcg accgctcgaa ctgctcgagc gcgcggtcgg gctggttcag gtagtaatag
     9301 gcgacgccga ggtccgtgct gacgttgggg tctttcgggt caatcccgag cgcggcctcg
     9361 taccaggtga cggcctcgcc gtagcgctcg gcatcgaagt acgcgttgcc gagctgcacg
     9421 cggctctgag cgtcagaggg gttcttctcg gctgcggcgc ggagcacctg cacgcgggcc
     9481 tcgtcgagcg ggggggcctg gggctgcgcc gcgggcgccg cgccgccggc gggctgcacg
     9541 gcggcggccg gcggtacgcc ggcgagggcc ctcggcccgg cctgctgagt gccgatgatc
     9601 cacccgacga tgaggccgaa gaacgccccg gcgatggcga ggacgatcga ttccggtttc
     9661 ataggatgat ggttggagac gccgacccgc cgatcataac agggtgcgtt tgaaccagtc
     9721 caccagcacc ccgacgagcc cggcttgccg cgccagcatg gtggtcccat gccccgcccc
     9781 gtccagcacc agctgctcgc gcacgcctgt cccctcgctc gacaggcgcc gcagcgtgat
     9841 catcacgtag gcatcctcgc ggcttcccac gagcagcgcg gggcggtcgc cgtagcggtt
     9901 gaggttggcc tgcgttcgca ggccctggta gtcgaggccg ggcgacagca gggcgatcga
     9961 gcggaccgtg ggatcggcgg cggccgcgac gacggccagg ttggcgccga tcgacgcccc
    10021 gagcagcccc accttcccgg gcgtgacctc gcggcgcgtc gcgaggtagt tccgggcggc
    10081 cctgaggtcc gcgacaatcc cgccgtcctt cgcactcgga cccgtcggcc tcgggtcgga
    10141 ggcgccgtga ccccgcaggt cgattgccag ggccaccagc cccgcgtcag ccaggcgcgg
    10201 gccgacggcc tcccagtcct ggcgcgaccg cgtctgcata tggatgagca cgaccgcggg
    10261 cccggggcgc gagggcaggt acagcgtcgc ggcgatcgac acgccgtccc cggtgacgaa
    10321 gtgcacgtgc tgttgagtcg aggccgaggc agcccacggc gacagtgcga gccacgccag
    10381 gagcgcgggc acgacgaggc gatgcccggt cacctgagca gctccaccat ttcacgcacg
    10441 gcccgctcga ggccgaccag cgacgcgcgg gagatgatgc tgtgcccgat gttcagctcc
    10501 tcgatcgtgc ggatcgaggc cacgggaccg atgttgaagt aggtgaggcc gtggccggcg
    10561 agcacctcga ggccgccagc ggaagccgcc tcggccgagc ggcggacctc ggcgagccgg
    10621 gcggcgcgcg acgagggaga ggcgtcggcg tagccccccg tgttgatctc gacggcgtcg
    10681 gcgcccacct ccttcgaggc cgccacctgc gcctcgtcgg gatcgatgaa gatgctgacg
    10741 cggatgcccg cctcccggag ccgccgggtc gccgcaatca ccgcggcgcg gttcgccacg
    10801 acgtcgagcc cgccctgcgt ggtgatctcg tggggttgct cgggaaccag cgtcacctgg
    10861 tcggggcgga cgcgcaacgc gatcccggtc atctcctcgg tcgcggccat ctcgatgttc
    10921 agcttggtgg ccaccatctg gcgaagcagc tccacgtcgc ggtccttgat gtgccgccgg
    10981 tcgccgcgca agtggacggt gatgccctgc gcgccggcca gttcggcgag cagcgcggcc
    11041 gcgatcggct cgggctcccg ggccttcctg gcctcgcgga tggtggcgat gtgatcgatg
    11101 ttgaccgcaa gtcgcatgat tcgctccgtc gcaagacccg cagacccgct ggccgctcga
    11161 cttcgctcga ggctggcccc tcgacttcgc tcggggcagg cggaggccgg cgccactagc
    11221 gaggcgtcgc gtcgggaagg ccgccgtcca ccgggatggt ggcacccgtc gtcggcgtct
    11281 gtcgcgtggc gaagaacagc acggcgttgg cgacgtggcg cgcggtcacg ggggccttca
    11341 gcaggttgcg gctccgatag taggcttcga gccccgcttc atcgaggccg cgggcgcgca
    11401 tccggtcggg gccgacctcg gcccagaggc cagacttcct gctgccgtcc gagaacacgg
    11461 cgtcgggcgc caccatgttg acccgcacgt cgagcccggc cagctcgagg ctggcgatgc
    11521 gggcgagctg gtgcgaggcg gccttggtgg cgctgtacgc gccgaacttc gcgccgggcg
    11581 caaacacgtt cttggtggag accagcacca cgtcaccgcc gctgccctgc cgttcgaaga
    11641 gccgggcggc ctcgcccagc accagcagcg tcccctccac gttcacccgc tcgagccgcc
    11701 tgaacgccga aaggtccatc gtcgccagcg acgagacgtg cgcgatcccc gcgttgacca
    11761 ccaccaggtc gacgccgccc cactcctcga cgacccgccg ccacgcctcc cccactgcgc
    11821 cggcgtcggt cacgtccatt ggcacgccga tgatggccgg gccgaacacc tccgacagct
    11881 cagtgaccaa cgattcgagg ccggggccgg gcaggtcggt ggcggccacg aggcagccgg
    11941 cctcgaggag cgcgcggcag atccccgaac cgatcgcccc cgccgcgccg gtcacgatcg
    12001 caacctcgcc cgtcagcgcg ccgccctctc gaccggcgag cttcgcgtgc tgcagcgtcc
    12061 ggtattccat gtggaacagg tcgcgctccg gcagaccctc gtaggtgcct gaccccgcga
    12121 tgctcgcctt cacggcgagc gtgtgcgcgg tgatgtcgcg ggcgatctcc gcgtcgcgca
    12181 ggtcgcgccc cgcgcagaac gcgccaagac ccggcgcgag caccacgcgc gggagcgcct
    12241 cgaaggcgtc cagctcgcga ggcatcatcg aggcgtgccg ctgcaagtag tcgcggtagc
    12301 gcgccgcgta ggcgccgatg gccgagcgga agacgtcggc gatggccggt ccgccaccgg
    12361 ccgcctcgag cgtcgcccac gcgggccagg ggcccgtgcg gatcagatgg tcggatgtga
    12421 gcggtcccgc cagggccgcc tcgcgcatgc cgggcgactc gagcagctcg aggacgtcag
    12481 ctgttgccag ggcccggacg acgacgcgcc tccgcctgcc ggtcgcctcg tccctcggtc
    12541 cggccaagag gccgcgcagc accggcgcgg cgtccgcgat cccctcacgc gtcctggcag
    12601 cctggtctgc cgaaacgaca cgagcacggg cgcgacctgt gaggcacgcc tcggccctgg
    12661 agacgagctc gatggtcgcc tcgtacgact cgcgggcgtc gcggccccac gtcacgaggc
    12721 catggcgggc ccataccatc gcccgtgccg ccggcgccgc ctcgaacgcg tcggccgccg
    12781 ccttggccag ctcgaacccc ggctcggcgt agggcatcac gatcacgcca tccccgagca
    12841 cctcgccggc aagcgcctcg ccgccggcgc ggttggtgag cgccagcacc gcatcggcgt
    12901 gagtgtggtc gatgaaccgg tgaggaagga aggcgtggac gagggcctcg attgacggcg
    12961 cgccggcgcc cgcacgtagc aactgccggc gcagctccca caccatctcg tcgtcgccga
    13021 ggcggtcgag ggcgcgaagc cggcgcaacg gcccgagggc caggcagacg tgcccgtccg
    13081 gtccgatcgc ggccaggtcg gcgcccgagg ccttgacgaa gagcgcctcg tgacggtcgc
    13141 cgaagacgtc ggaggtttca gccttgacgg aggtattgcc cccgccgtgc agcaccagcc
    13201 ctggctcggc gcccagcaat cgcgccgagt aggtgcgcaa cgcgagggcc tcgccccagg
    13261 cggggccgtg caggtcgacg aagcggcgag cctcggtttc gctccagagt gacttcatga
    13321 gcgcaatcag cccacgggct gaacgatggg gcaagcggtg caaccgtcac gcccgtaccg
    13381 gacaggcggg acgccggttc gggacaggcg gaacgcctgc tcggcacagg cgggacgccg
    13441 gttcggcaca ggcgggacgc ctgtgctact gggctcccac cgcggcgagc atcgcccggg
    13501 cgttctcggc cgcgtggcgg ccgtcgaaca ccgcgctgcc agtgacgagc aggtcggcgc
    13561 ccgccttggc aatctccgcc gcgttggcgc gcgtcacgcc accgtccacg cagacgatca
    13621 ggccttccct ccccgagccg gccgccaggt cccgcacctg ccgcacccgg tcgagcgtcg
    13681 cggggatgaa ggcctgccct ccccaccccg ggttgacggc cagcagcacc accatctccg
    13741 cctccggcag cagcggctcg atcgaggaga ccggggtgcc ggggttgagc gcgatcccac
    13801 ggaccacgcg gcgcgaaggg ttcgcggcgt gcggcatctg gccgagcgac tggagcacac
    13861 ggtgaacgtg gatcgacgac tccacgtgga ccgtcacgat gtcggcaccc gcggcgacgt
    13921 actccctcac cttctccagg ggatcgcgga tcatcaggtg cacgtccttc aacagcggcg
    13981 tccggcgcag ggccttcacg aaaggggggc cgacggtcat catcggcgtg aagcagccgt
    14041 ccatcacgtc cacgtggacc accttcacgc cagcgtcctc gagcaccttc agctcggagc
    14101 cgagcgagag gaggtcggag gtcaggaggc cgacgctgat ggtcggacac gccgccgaga
    14161 gggcggctgc cggcgtgccc gtcatactgc cgcccgatcg cgcttcgcct gtgccgcgag
    14221 ctcctggtac agctcgtacg cgtgctcggg cttcatcagc tcgtggacca ccttctgcac
    14281 cgcctggatc atcgccgtcg gggcggccga ctggaagatg ttgcggccca tgtccacgcc
    14341 ggcagccccc tcgtccacgg cccggaacgc catggtgagc gcctcgagct cgggcatctt
    14401 cttcccgccg gccatcacga tgggcaccgg gcacgaggcg gtgacggtct cgaacccgtc
    14461 cgcgacgtag taggtcttga cgaactgcgc gccgagctcg gcgcagatgc gcgtggcgag
    14521 gcggaagtac tgcgcgtcac gaaccatgtc cttcccgacc gcggtcaccc cgagcgtggg
    14581 gatgccgtag cggttggcca tgtcgaccat ccgggtcatg ttgttgacgg tctgggtctc
    14641 gtactccccg ccgatgtaca cctggatggc catcgcggcg acgttcatcc ggaggccgtc
    14701 ctcgatgtcc accgcgatca gctcgttcga cagctccttg cggatgctgg ggccgccgct
    14761 ggcgcgcatg acgacgccgc ggccgaacga cggcgggatc gtcgtgcgca ggatgccgcg
    14821 cgtgagcatc agcgcgtcgg cgtacggcag cagcggcacg atgttgacgt cgatccgctc
    14881 gagcccggag gtcgggccca ggaagtagcc gtgatcgatc gccagcatca ccgtgcggcc
    14941 ggaccggggg ttgaagattc gcgcgagccg gttctgcatc ccccagtcga gggcgcccgc
    15001 gcccttcagg aagaacggct cgttccgggc ggggacgtcg aggtggtagt ccttcgtgtc
    15061 cttgaggttg tccaggtcgg ccatgctcag gctgctcctt cggccggctc gccgaggtga
    15121 cgccgcgcgg cgtcggcaat ctcggtcgcc caggcggtga tctgatgctg gtcctgaccc
    15181 tcgatcatga cgcgaagcag gggctcggtg ccggagtacc ggaccaccag gcgcccgctg
    15241 gcgccgagcc gccgctcgac atcgtccatc acggcggcaa tctcggggat cccttgcagc
    15301 tcgcgcttct ccttcacgcg gacgttgacg agaacctgcg ggtaggtgac cagctcgccg
    15361 gcgagatcgc ccagctcgcg ccccgtctcg gccatcacct gcaggacgtt gagcgcggtg
    15421 accaggccgt cgccggtgaa caggaagtcg gagaagatca cgtggcccga ctgctcgccg
    15481 ccgagcgaga ggttgcgcgc gatcagctcc tccatcacgt gcttgtcgcc gaccgggcac
    15541 cgcaccagcg cgatccccga ttcgcggagc gcgatctcga ggccgatgtt gctcatgatg
    15601 gtggccacga tcgcgttgcc gcgcagccgg ccggccgact gcaggtggcg cgcggccagc
    15661 agcagcacgc cgtcgccgtc cacgatgcgt cccgaggcat cggcgaagat ggcgcggtcg
    15721 ccgtcgccgt cgaaggcaac ccccattcgg cagccctgcg cgacgaccgt gcgcgccagc
    15781 gcggccggct tggtggatcc gcattccagg ttgatgttgc gcccgtcggg ctcgcagccc
    15841 agcaccacca ggtcgaaccc cagctcgcgg aacagccgcg gcgcgatcgc cgaggtggcg
    15901 ccgttggcgc aatccaccgc cagccgcaaa cccgcgagtc tgccggcgga aggcagcgca
    15961 atccgcgcgt gctcgaggta cagcgtcgta agatcaaggt cctcgagcgg cgccggctcc
    16021 ccggccggca cctgccacga ccgatccgcc acgacggcct cgacggcccg ctcgagctgc
    16081 tcggtgaact tctcccccgc ccccgagaag accttgatgc cgttgtcctc gaacgggttg
    16141 tgggacgcgg agatcacgac gccggcgtcg aacgcctgct cgcgcgccag gtacgccacg
    16201 gccggggtgg agatgacgcc ggcactgacg atcgagccgc cggcgctcct gaccccgtgc
    16261 gcgaactccc gcacgatcca gctccccgac tcgcgcgtgt cgcggcccac caggaggcgg
    16321 tgccgcccgt catccgccgg cagcgcccgg acaagcgcgg cgccgaggcg tcgaatggtc
    16381 tctgagtcca ggggataggc gccagcccgt ccgcgaacgc catcggtgcc gaagagtcgg
    16441 tgtgccatgg ttgctcgaag ggacaggcag gctagcggac cacccgcacg cgcacccgtg
    16501 cgggctcgat gcggagcacc tcgtagtcct gcgacggtgc gacccggacc gggagattgt
    16561 accggcccgg tccgaggccg gcaaggtcca cgaaggcacc gaccgcgtcg gtccgcagct
    16621 catccaggac ttccttgcgg cccttggtca ggaccgtcac caccggcggg accgactgcg
    16681 cgctcagccc ccgcccggcg ttgtggagac gcaccgggac gttgagaacg gcgcgctgca
    16741 ccggcagggg gaggatgtcc accgtgatcc gcgcattcac gggtttcagc aggcgcaccc
    16801 tggggtcggc aacgccgacg gtggccgact cgcggaccac ccggcgcgcc ccctcgatcg
    16861 acactgggtc ggtgatggcc tccgtcacgt cgcgaagggc gctctcgggt ccgaccacga
    16921 cgaccgtcgc cgggtcggac gtgatggcgc cgaccttgta cccttcggcc ggctcgccct
    16981 cgatcgccgg cacgacgctg accgtgcgcg acccgctccg ctcgaagacc agggagatgg
    17041 tgcccggcga cacctgggtc acctctacgc cgaatggcgc gcggacgttc tgcggcgtga
    17101 ggtggaagag ccgccggccc ggccgggccg acgacagatc gagcacggcg accacctcgg
    17161 cggccgaaag ctggcgcagc aggcccccgg cgccccgcac gcgcacgtcc acggccgtgg
    17221 gggggttctc gaccaggacg agactttccg gggcgttctg caactcgagc ggcgcacgca
    17281 cgctccgctc cacggtctcc tcgccggcga cgacgaacca cagcgcggcg gcgatggcca
    17341 ccgagatgaa cttgaggccg aggtggcgga acgggtggta gtaggccatc agtcgtacag
    17401 cccgcgcctc tcgcggggcc tcgcctcgcg ggcggccagc agctccctga gccgcgtcct
    17461 caggatgtcg gccgcaacgc ccacctcgat gcgcccctcg agcgcgaccg agatccggcc
    17521 ggtctcctcg gacacgatga cggccagcgc gtcggtctcc tcggtcaggc cgatggcggc
    17581 gcggtgcctg gtgccgaagt ccttgctgac caccgggttg atcgtcagcg gcaggaagca
    17641 ggccgcggcc gccacccggt tgccctggac gatgaccgcc ccgtcgtgca gcggggtgcc
    17701 cggcttgaag atggtgacca gcaggtcgtt ggtcaccgtc gcgtcgagcg ggatgccgcc
    17761 ctcgatgtag ttgcgcaggc cgatgtcgcg ctcgatcgcg atgatcgccc ccacgcgctg
    17821 ggacgcgagc accccggccg cgaccgcgat ctcctcgatc acgtcgtcgc ccccttcgcc
    17881 gcggccgaag tagccggcga acggcgtgcg gccgaagtgc gcgagggtcc tgcggatgtc
    17941 ggcctggaac agcacgatca gcgcgaagac gacgtagccg atcatgttgc ggatcagcca
    18001 gttcaccgtg gtgaggtggc ccaggcgcga gaggtagaag agcgccagca ggaaacccgc
    18061 gccgagcccc atctggaccg cccgggtccc ccggatcagc ttcaggatct cgtagatcgc
    18121 caccgacacg atcaggatgt cgaggacatc ccaccagccg agacgctgaa gtatgccgag
    18181 cagtgcgtcc atgcggtggc gccgagggtt gaagttgggg cgttaccggg cgccggggcg
    18241 acccgcaagg cggatcgcgt cggcgacgcg cgcgacctgc accatctcgg cgacggcgtg
    18301 gacgcgcacc acgtgggcgc cgccgaggat cgccgccgtg acggccgccg cggtccccca
    18361 gtcgcgctcg gccgcgggac gctcgccggc ggccgcctgg aggaacgact tgcgcgaggg
    18421 gcccacgagc aacgggcgcc cgagggcggc aagctccctc aggcccgcca gcacctcgta
    18481 gctctggccg gcgtgcttgg cgaagccgat ccccgggtcg agcacgatcg cctcgcgggg
    18541 gacgccggca cgggcagcgc gctccatcgc ctggcccagt tcgtcccgga cctcggcgac
    18601 gaggtcgccg taggccgcct cgcggtacat ccggtcgggt gtcccgcgag tgtgcatcag
    18661 cacgaggccg gcgccggcgc gggccacgac gcccccgagt gccggatcat acattagtcc
    18721 gctgacgtcg ttcacgaggc cggccccctc gtcgagggcg gcgcgggcga cgcccgcctt
    18781 gcgggtatcg acggagatcg gcagccggac gcgcgcgctc acggcgcgga gcgcgggcag
    18841 gaggcggcgc agctcctcgt cctcgggcac caccgccgac cccggcctcg tcgactcggc
    18901 cccgaggtcg aggatgtcgg cgccgtgctc ggccgccgcc tgcgccaggt cgagcgcccg
    18961 ggccggatcg acggatggcg acgcctcggc aaacgagtcg ggcgtgacgt tgatgatggc
    19021 catcacgagc gtccgatccc ccagcacgag gggatcgccg cccgcgacgg gcacgacgta
    19081 ggaggtcagg gggaacacgc gcgtgtcacg ggaacccgcg gaacggcagg cgcagaatgc
    19141 ggcgcgggct cgagccgcgc acgcggacgc gtgcgccgtc cccggttcta cgtcctccgc
    19201 ccggcccgcc tggcgggccg tctactcctg gggcaggggc ttctggatcg gctgcaacgg
    19261 cgggacgatg gcgccccggt cgcgctggcg ggctcgcgcc tcgtcctcgt cggacgccgg
    19321 aaccgacggc cgcgacacga cgtcctggat cgactcgccc gccgccaggc gcttgacctg
    19381 ctcgccgtcc agcacctcgt gcagcagcag cgcgtcggcg atgcggacga ggccctggcg
    19441 gttctcggca agcatcgcgt gcgcgcggtc gtagttggag gtgacgatgg ctttcacctc
    19501 ctggtcgatg cggatggcgg tgtcctcgct gtagtcctgg tgctgcgcga tctcgcggcc
    19561 caggaagatc tgctcctcct tcttgccgaa ggtgagcggc cccatcgcgg ggctcatgcc
    19621 ccactcgcag accatcttcc gcgccaggtc ggtcacgcgc tcgaggtcgt tgcccgcgcc
    19681 ggtggtcacg ttgccggtga gaatctcctc ggccacgcgg ccgccgagca ggatcgagat
    19741 ctggtcgtcg aggtagggct tcgagtagtt gtgcttctcg tcaacgggca gctgctgcgt
    19801 gaggccgagc gccatcccgc gcgggatgat cgtgaccttg tggatcgggt cggcccccgg
    19861 cagcagcacc gtgagcaggg cgtggccggc ctcatgcacg gcggtcacct gcttctcggc
    19921 gtcgctgatg atcatcgagc ggcgctcgct ccccatcagc accttgtcct tggcgaactc
    19981 gaagtccatc atccggacga ccttctggtt gtagcgggcg gcgtagagcg ccgcctcgtt
    20041 caccaggttc gccaggtcgg cgccgcagaa gcctgccgtt gcccgcgcga gcaccccgat
    20101 gttcacgtcg tcggagagcg ggatcttccg cgtgtggacg gcgaggatcc cctcgcgccc
    20161 cttcacgtcg ggccggttga cgacgatgcg ccgatcgaat cggcccgggc gcagcagcgc
    20221 cggatcgagg acgtcgggac ggttggtcgc cgccacgagg atcacaccct cattcgactc
    20281 gaacccgtcc atctcgacga gcagctggtt cagcgtctgt tcgcgctcgt cgtggccgcc
    20341 gccgagcccg gcgccccggt gccgcccgac ggcgtcgatc tcgtcgatga agatgatgca
    20401 gggcgcgttc ttcttcccct gctcgaacag gtcgcggaca cgggaggcgc cgacgc
//
LOCUS       DAOPQX010000021        20278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3262_length_20278_cov_4.914157, whole genome shotgun sequence.
ACCESSION   DAOPQX010000021 DAOPQX010000000
VERSION     DAOPQX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3262_length_20278_cov_4.914157"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKK95_02250"
     CDS             complement(<1..1043)
                     /locus_tag="PKK95_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17055.1"
                     /translation="MNVRTATVLICTYNRAPFLRETLQALVEAPRRDDYEAEILVVDN
                     NSDDGTRQVVEEAARAASLPVRYAFDARQGKSFALNVGLRLAHGDIIAHTDDDVVPGE
                     GWLDRMVDNFRARDITFAFGKVLPRWGAVPPPELLLPAAHRIWGPLALLDYGDQPVAY
                     PDDGIGRRLPIGANLGFHRRALAEVGGWRTDLGKVDNSLISGEDHEVFHRLRSHGQYR
                     GWYDPHLVVSHYVPASRLTRQYFRRWFYWHGKTMARMDSVLYDLDFSRVPHVFGAPRF
                     IYRQALAQAWTWARKMGRKDGLALLIEDALPSANQGLMVDAVHDGEQLRAQAMQERIQ
                     DIGRKGIVLGVEK"
     gene            complement(1040..2008)
                     /locus_tag="PKK95_02255"
     CDS             complement(1040..2008)
                     /locus_tag="PKK95_02255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOC17056.1"
                     /translation="MPLSVVIATFNRAPLLRECLARFERQAFRPGDEVVVADNGSTDA
                     TPDVLREAAARLPVPLRTVFEAKPGKSRAVARGVEAARGDILAFTDDDVIVADDWIER
                     LHAIFEERDVDLVGGRVLPRFMGRVPSWLKLSDGRGYNRMASPITLLDYGPERQPLGR
                     RAAPGGNLAIRRSVFEALGGFPRHLGKLRGTLLSGEDHLLSERVVASGYRAIFEPSLT
                     VQHLVPRSRLRFRYFLRWFFWSGVTHAVLDAGREPGEHARPLTRYHAGQGVKMLGKAA
                     LAALAGGWTGAAREATEAAFSAGYVWAGLSPRVRQWIAAGRGAEAA"
     gene            complement(2013..3587)
                     /locus_tag="PKK95_02260"
     CDS             complement(2013..3587)
                     /locus_tag="PKK95_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOC17057.1"
                     /translation="MMPLRPGRSIAVNLATGTATRYVLLAVNIGIGIWMMPFSVGHLG
                     KSDYGLWMLVVSMTAYFQLFDLGYGNGLVRQITDADARGDERAINELVSTFAVVYAAI
                     GAVALLATAALAAWVVPRFPNLSAGQVTTAQWVTVVLGIRIAVGFPMTVFGAVTTSRQ
                     YFALSSSIAIAVSLLAALVTYLVLEAGYGLRVLVPATTAVNLLAYVAYARAAWFAYPG
                     LRLAPSLFSPRHLREVTSYSLYLFLITVAAHLGYNLDNVVVGAFMGTSAVAVYAVASR
                     LADYQRQLSNQFNGLLFPVVVGLGAQNDRARLRATMLHGTRLALGLALAVTIGLLAFA
                     PPLVRAWMGPTFEDSLAPLYALAIASVVLVALGPLGNILLGTGRHRLVAFSSLAEALL
                     NVGLSLLLVRRWGLAGVAAGTAIPVVAMNLLVLLPAACRSLDVRPGRFVREAAAPALL
                     PAVPALAIAVVLRLYVPPASLLAVAAAGALVGLVYLAGFVSFGLPAGDRRQYVSYIRR
                     AAGSTRQPVAAVESGG"
     gene            complement(3584..4516)
                     /locus_tag="PKK95_02265"
     CDS             complement(3584..4516)
                     /locus_tag="PKK95_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC17058.1"
                     /translation="MTVISVLVATHDRRRLLGETLEAIARQDWPRDRLEIVVVDNASS
                     DGTPEMVAAFAARRDVPAVRLLHEARPGKSHALNQGVAAALGRILVCTDDDVLPDPGW
                     VRAMVEAIDQTGADFAVGRIRPRWEIPPPPWISPRLYGVLAVPDNGPERFEIRHGLNE
                     HAMPIGANMAVRAEVVREIGGWRADLGKLRHTLRSGEDHEFYLRMLRAGMRGVYEPRA
                     SVAHFVPAERLRRAYFLRWLHDNGRTVSAIEREFPTTSRYLLGVPRYLWRQAGVELAR
                     FGRAALGRQAPERFASLTRLLWFVGYLRGAWQGR"
     gene            complement(4513..6765)
                     /locus_tag="PKK95_02270"
     CDS             complement(4513..6765)
                     /locus_tag="PKK95_02270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17059.1"
                     /translation="MAAPPSPHHRPAAPQVSVIIPAYNAAGSIGEALASVLAQTFTDY
                     EVIVVDDGSTDDTAEVVGNIGRDEISLIRQENGGPGSARNTGIRASRGRLIAFLDADD
                     RWLPHKLEAQVAYFHAYPQTGLLHTATTGRPGDASSVAVFDSHPRPPRHVFCELFHTD
                     YVIATLTVMVRRDVLDSVGLFDERREVHVEDWDLWLRIAASYPVGYLAQPAAVRVLGG
                     GMSADIEKTFRGQAEAIAKNVATCRSGCSLHRAEPAGCLRRRWYRFYWELGYARLREG
                     DRASARAAFWRALGRRPLSTSAWLQLAATFAGERSRTGARRVLAALGQPNALAQPHRD
                     SSVPESLVDRTAYRRTRRAVVRTLHVAETAVYRLRAGERRRILFEAASPMSFAIFRPL
                     YERLREDPRFEFWFTAGRTWDVEQLYAHVGITRNVVPARRAVWLKVDAVVNTDFWPST
                     WLYRRTRRIHLFHGVAGKYGLDAPVEIAPTVRTYSRLLFPNEDRLNRYVEAGLVPPGS
                     PVGQLTGYPKLDALVDGSLDGGAILAGLGLDPGRPTVLYAPTWSPHSSLKRVGHSLPP
                     ALEAAGCNVIIKLHDRSLDLAERASGGVDWAAVFAPYRDHPRIRLATGADATPYLAAA
                     DALVTDHSSVGFEFMVLDRPIVVIDCPDLLAHARVPKSKVADLRRAAEVTSDVRDTVE
                     AVVAQLADPSRHHAERVETALRFFYRPGTATARAVACVYEVLELEAPLAEPAAHAHSR
                     ERALATLSEG"
     gene            complement(6767..8332)
                     /locus_tag="PKK95_02275"
     CDS             complement(6767..8332)
                     /locus_tag="PKK95_02275"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC17060.1"
                     /translation="MERLAWGHGAVPGRREVPEVRFETRELRESEMPSVVADPLPGES
                     SDWAFIGLLAFTAVLFFRPQDQIPALAALHLAEVSALVGLTAMAAKRLARGLPVFRLT
                     PEVVGLLAFGAVLVGTVPFSFWPGGSVNVIQNLYGKVVLITILIINSINSPRRLQHFS
                     RLIVLSLAYLSVRAVFDYLRGVNLIEHGRVAGAVSGIFGNPNDLAMNMVSFLPFPILT
                     AMVVSRLRRGAPSSAPGDSAPNGPAGQQAAAAAPGRRSRFRRDPGWFTSLLYAGCAGA
                     MFLTVVFTKSRSGFLGLLAMLVVLVFYGRQLRRGFAVAAIAGGLLAIPALPSSFWDRM
                     AGITDPAKDDTGSREARRTVMKEAWWVFLERPLTGIGAGQFQNYNPPWKKERWRQSHN
                     LLLQVAAETGIFGITAFVFLIVVGFRAALSTRRTLTRLARAPDVQASPGVERDVRVLR
                     LHTTALVAGLTGWFVCSLFSSIAYNWTFYYLIGLAAVARDLTRPIAARVPAAPRARAA
                     EPMTPAPAAWARG"
     gene            complement(8432..9538)
                     /locus_tag="PKK95_02280"
     CDS             complement(8432..9538)
                     /locus_tag="PKK95_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC17061.1"
                     /translation="MKWVFWISAIIVAYVYAGYPLVLAAWAPLAARRRARAARRRPAS
                     PPEGIEPPVSIVIAARNEAAVLRGRIENLLALDYPAGRREIVVVSDGSSDETPALLAS
                     YAPAVRSICLPPSGKAAALNAGVGAASHEIVVFADARQRFAPDAVRRLVARFADPEVG
                     AVSGELVIDAERGDGAAAASAVGDGVGLYWRYEKWIRRRESAVGSVVGATGAIYAVRR
                     ALWRPLPPDTLLDDVLAPMRIALGGYRVVFEPSAIAFDTASPDAVTERRRKVRTLAGN
                     YQLVRLEPRLLVPGANPLFVQFVSHKMGRLVVPYALIALLVSSAALASSGAVYSLALV
                     VQVTFYVLALHGAAAAASGQRKAHGKELVNAELD"
     gene            complement(9555..10850)
                     /locus_tag="PKK95_02285"
     CDS             complement(9555..10850)
                     /locus_tag="PKK95_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC17062.1"
                     /translation="MYESYFQLRERPFALSPDPDYLYPSRVHREALDYLRYGIETQAG
                     FVLITGEIGSGKTTLLQALLRGLDGQTTVVRLMNTMLEPRELLEAIAIDLGIPPSPSG
                     KPALLRDLSIRLVEERAAGRLVLLVIDEAQNLGLPALEEIRMLSNLETEKSKLLQIVL
                     VGQPDLRQQLDLPRLEQLRQRITVSYHLQALDADETERYINHRLRRAALGAPLQFPRE
                     VTDRVYARARGVPRLINVICDATLVYAYAEDRRAIEAALMDEVFAELEATGVLRPETE
                     RPGDPAGPVRPPLSPRQEAAIPSAREEEVARERQLAAQQAEIRAREDQLARRERQLAE
                     QQRVLAEEYRLFRSRQAREMTRASADPRRAAKTWPPARPGGRVEPPPRVGATAQAASR
                     PRPYVPPDAGDWDPRQRRGFWDRVKHAFLGGPVGAVEDR"
     gene            complement(10843..11676)
                     /locus_tag="PKK95_02290"
     CDS             complement(10843..11676)
                     /locus_tag="PKK95_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOC17063.1"
                     /translation="MRAKGLAPRAIILAAGSGRRLHGAGGDCPKCLLRVGSATLLHRQ
                     LAALRACGISDITVVAGYQEPRVAEACGTTARVVENGRYAETNSLFSLWLTRDLLTDG
                     FLVLNGDVLFHPQLLRDLVCSRVEDALLVAFRDSLLEPFGVEEMKVQVRGGRVADIAK
                     TLPAEHADGENVGIAKFGPAGARQLVAEMDAIVSRGGVREWAPRAFQAFARVRPLHAI
                     GTRGFPWIEIDFPEDYARASQEVLPSIEATFDSAAATPAVPTLAPEPMEAEWRPQPGH
                     V"
     gene            complement(11676..13043)
                     /locus_tag="PKK95_02295"
     CDS             complement(11676..13043)
                     /locus_tag="PKK95_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOC17064.1"
                     /translation="MTLFRRSISSRGAAAFAGEVFLIAGSIAIALHLNDRAASADGLW
                     WKAAATAALWQLVLYYNDFYDLTLVHSSRELIVRLLQGAGAAAIVLAGLYALVPALDL
                     GSRVILTSLLLFLVAVLGWRLLLFWVAGVEALEERLLFVGTGGAARTVARQIAAQRDF
                     PYRVVGFVDDEPAGAGDREGWLLGNTTDIPRLVEQYAIDRIVVGLADRRGRLPVQELM
                     QAKLSGIRVEDATATYERITGKLLVDNLRPSWLIFSEGFRVSAWTRLVKRAFDLALAG
                     AGLVIASPLLVLAAQAIWIEDGFPVIYRQQRAGRNGRPFTLYKFRSMRHDAEGETPVW
                     AADADPRVTAVGRALRTTRLDELPQLWNVLRGDMSFVGPRPERPFFVAQLAAQIPFYE
                     QRHAVRPGITGWAQVKYRYGASVEDALEKLRYDLYYIKHLSIGFDVTILVDTVKVILF
                     GKGAK"
     gene            complement(13054..13992)
                     /locus_tag="PKK95_02300"
     CDS             complement(13054..13992)
                     /locus_tag="PKK95_02300"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOC17065.1"
                     /translation="MSHIQRVLDKAQRDGFVRRTAPVAPAERLVAGQAAARPASQERA
                     SALPPPAPQSPPPAPTVVPNPLVAKVRTADPDAINSLLVAALAPESPAAEQYRALRTR
                     IAHTENGRQYRTLLVTSPGARDGKTVTAVNLALTMAQEFHRRVLIVDTDLRKGRLHRF
                     LNVPSSPGLCEVLTGEATLEEALVSLPAYHLTVLPAGGPTGLPAEMLGSAEMRRTLDL
                     LRTQYDRIVLDTPPVVALADVGVLAPVADAVLMVVRAGRTSRPNIDRALREVHPAPVL
                     GLVLNDVDDLALEYPGYRPDGSATHRLRFPARRQSA"
     gene            complement(14017..15462)
                     /locus_tag="PKK95_02305"
     CDS             complement(14017..15462)
                     /locus_tag="PKK95_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOC17066.1"
                     /translation="MEEARFHPLDYLSVVRRRKWWFIVPATLAVLVGIGLVLFLPKQY
                     RSTATVGIAAPAVSGDLAASAALNRDDRIRALSQQLLSRQVLARVVRAEGLDASEVGL
                     DGAIGRLRGGVQPISFPTPVAPTANGQPSLDLFYVSYVDGSPEAAQRITNRLVEVFVD
                     ETSRSRTARAEDTTVFLDAQLRQAQQRLDDTEAKLREAKQANMGRLPEQTDANLQSLN
                     GLRQQLEATNMALRSEQDRLSVIERQIEAMQQGVETLPLLNGSTTPATPELRVQALEQ
                     QLAAARMSYTEKHPEVLRLVDELKAARATAAAERERPAEDRLAHLKNDPAYRQLLADR
                     ENGRLRVRELQRAESQARAQIGLYQTRVESAPMVEQQMLALQREYDVQKKQYEDLVAR
                     REAARMTESLERRQASEQFRVFAPASWPGAPFKPDRMRLLLGALLAGAFLGGLGVVGR
                     EFLDRSVHDSVTLAREYELPVLGEISRIRAA"
     gene            complement(15462..16664)
                     /locus_tag="PKK95_02310"
     CDS             complement(15462..16664)
                     /locus_tag="PKK95_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17067.1"
                     /translation="MRVAPRFAAPVLAALVLAAPPAWAQFPLQEVREPGQTPGWTLTP
                     SVALSSGWDSNVSMVGVDEPETNGSLSVISPSINAAYLAKKHWFGAGYTGSFSLYHQL
                     EGLNTFDQYLRVDTRHLLSKRVTLNLHDSFAAVPTTDAIMLSDLPFVRTGSRMNDAGA
                     SLSVALDARTKATASYDFTWVAFDERSPYAGLLRGGTAHAFGGGVQRQMGARWSLGGS
                     ASYQRTLMARFGQGVGIVEGFGTVAYDATRTLSLSASLGVSHVGATNRNTARTGPAWN
                     VSAVQRFRRATLSGAWSRSFVPAFGLGGSIQNEQVDVVLAMPVARNRLYWQAGLSWRR
                     HDPLLAEELTRHALWLQTSVGYAVHRLVRLEAFYARSQQDSLRPGGKVHRDRLGVQVV
                     TSKPMRIK"
     gene            complement(16661..17302)
                     /locus_tag="PKK95_02315"
     CDS             complement(16661..17302)
                     /locus_tag="PKK95_02315"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOC17068.1"
                     /translation="MKRSPALTALMLNALVLAAGTASAAEQRPSKPAAPPPQASASPA
                     PAAPSRSAATSSDYRLGAGDKLRIEVYKEPQLSQSLQVRPDGKITMPLIGDLPAVELT
                     PIELRDRIADALREYVTNPVVTVIVVEALASNIYVMGEVAKPGPIPLQGPMTALQAIA
                     MAGGFKDFANTKDIRILRRHRTGVETIKFNYREAIRGNGTPIYLRAGDTVIIP"
     gene            complement(17506..19050)
                     /locus_tag="PKK95_02320"
     CDS             complement(17506..19050)
                     /locus_tag="PKK95_02320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC17069.1"
                     /translation="MKKPFVAIVDDDSAFSNYLRTFLSLRGYEARTYSRGDELLAAIR
                     QADPPDVVLLDVMMPGLDGLATLRALKGARPETQAIMLSGREQASTIVEAVRLGAADY
                     VVKPDDPEGLGEIALDVAIKNALEKNRLVSELTELRQQLSDDQDRAFLFWGNSDQMRH
                     IANVIEHVADSDVTVLIRGESGVGKELVARAIHQRSPRRNKPLVKVNCAALPTELLES
                     ELFGHEKGAFTGAANTRIGKFEQADGGTLMLDEIAEMKAALQAKLLHVLQDGEFTRLG
                     GNKRQTVDVRVLAATNRDLEAMMVAGDFREDLYYRLKVIEVTVPALRERRDEIPHLTD
                     FFIARYSKKYNRPAPTLSDEVRQLFLSHDWPGNIRELENMIKRLIILQDEQMIVRELT
                     AAASRTPAYAPALAMSVGAGAGAGPPAYQPAQSVPPAAAVPPQPEPLAEGDELEPAPA
                     AAPPPEEPRTLADIARAAAMKAERAALEQTLDQVRWNRRKAAQLLGVSYKTLLNKIKE
                     CGISQR"
     gene            complement(19047..19751)
                     /locus_tag="PKK95_02325"
     CDS             complement(19047..19751)
                     /locus_tag="PKK95_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC17070.1"
                     /translation="MTDQRDHCARLLSLAVHEFRTPTAVVSGYLRMVLRHFGEGLTDQ
                     QRKLLEESEKACGTLSQLLADFSDLSRLEQGTLGLTREALPLGALLAQVASNVQEGRD
                     RGLTVEVDARADLVVEADRPRLSEAIASLMAAVLRERLQGARVVAVARLAGEPPSALV
                     AFGDAETAAGLLDDVEARQQRFDEYRGGLGFRLPAAARIIEAHGGRLASPVAERGRLS
                     ILLTLPAAVARAENAG"
     gene            19953..20225
                     /locus_tag="PKK95_02330"
     CDS             19953..20225
                     /locus_tag="PKK95_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17071.1"
                     /translation="MTFTRASRAALAALALVVLLAGTSIAVAGWMPGAGSQPQYTVAS
                     KEMDTIGTGTVVAAPTSRLKGISEKLMLVAVGGLLLGLAAAVRRTA"
BASE COUNT         3062 a         7020 c         7399 g         2797 t
ORIGIN      
        1 aacttttcga cgcccaggac aatccccttc cggccgatgt cctgaatccg ttcctgcatg
       61 gcctgagcgc ggagctgttc gccgtcatgc acggcatcaa ccatgaggcc ctggttcgcg
      121 gagggcaggg catcctcgat cagcagggcg aggccgtcct ttcgtcccat cttccgggcc
      181 cacgtccatg cctgggcgag cgcctggcgg tagatgaacc gcggagcgcc gaaaacgtgc
      241 ggcacccgcg agaaatcgag gtcgtagagg acggagtcca tcctggccat cgtctttccg
      301 tgccagtaga accagcggcg gaagtactgg cgggtcagcc gcgaggcggg gacatagtgt
      361 gagaccacca ggtgcgggtc gtaccatccc cggtactgcc cgtgcgaccg gagccggtgg
      421 aagacctcgt ggtcctcgcc ggagatgagc gagttgtcca ccttcccgag atccgtcctc
      481 cacccgccga cctccgcgag cgcccgcctg tggaagccca ggttcgcgcc gatcggcagc
      541 cggcggccga tgccgtcgtc ggggtaggcg accggctggt cgccgtagtc gaggagggcc
      601 agcgggcccc agattcggtg ggccgccggc agcaacagct cgggcggggg gaccgcgccc
      661 catcgaggca ggaccttccc gaacgcgaac gtgatgtcgc gcgcgcggaa gttgtccacc
      721 atgcgatcga gccacccctc gccgggcacc acgtcgtcgt cggtgtgggc gatgatgtcg
      781 ccgtgtgcca ggcggaggcc gacgttgagg gcaaagctct tgccctggcg tgcgtcgaac
      841 gcgtagcgga ccgggaggct ggcggcccgt gccgcctcct cgaccacctg gcgggtgccg
      901 tcgtccgagt tgttgtccac cacgaggatc tcggcctcgt aatcgtcgcg gcggggcgcc
      961 tcgacgaggg cctgcaaggt ctcccggagg aagggagccc tgttgtaggt gcagatcagg
     1021 accgtcgcgg tgcgcacgtt catgctgcct ccgcgccgcg ccccgcggcg atccattgac
     1081 ggaccctcgg gctcagccca gcccagacgt agccagcgga gaacgctgcc tcggtagcct
     1141 cgcgagcggc ccccgtccat ccgccggcga gcgccgcaag agccgccttc ccgagcatct
     1201 tcacgccctg tccagcgtgg tagcgcgtca gcgggcgggc gtgttcgccg ggctcgcggc
     1261 cggcatcgag gacggcgtgc gtcacgcccg accagaagaa ccagcgcagg aagtaccgga
     1321 agcgaagccg cgaccggggg accaggtgct gcacggtaag agagggctcg aagatcgcgc
     1381 ggtagcctga ggccaccacg cgctcggaca acaggtggtc ttcgcccgag agcagcgtcc
     1441 ctctcagctt gccgagatga cgcgggaacc ccccgagcgc ctcgaacacg gatcgtcgga
     1501 tcgcgaggtt cccgcccggc gccgcccgcc ttccgagcgg ctgccgctcg ggcccgtagt
     1561 cgagcagcgt gatgggagag gccatgcggt tgtagccccg gccgtccgac agcttcagcc
     1621 acgacggcac ccggcccatg aagcgcggga ggacgcgccc tcccaccagg tccacgtcgc
     1681 gttcctcgaa gatggcgtgc agccgctcga tccaatcgtc ggcgacgatg acgtcgtcgt
     1741 cggtgaaggc gaggatgtct ccgcgggcag cttcgacgcc gcgcgccacc gcgcgcgact
     1801 ttccgggctt cgcctcgaag accgttcgca gcggcaccgg gagccgcgcc gccgcctctc
     1861 gcaggacgtc cggggtggcg tcggtggaac cgttgtcagc gacgaccact tcgtcgcctg
     1921 ggcggaaggc ctgcctctcg aagcgggcga ggcactcccg cagcagcggc gcacggttga
     1981 aggtcgcaat cacgacgctc agcggcatcg cgtcatcctc cgctctcgac cgcggcgacc
     2041 ggctggcgcg tgctgccggc cgcccggcgg atgtaggaga cgtactgccg gcggtcgccg
     2101 gccggtagtc cgaacgaaac gaagcccgcc aggtagacca ggccgacgag ggctcccgcg
     2161 gcggcaaccg ccagcagcga agccggtggg acgtagagtc gcagcaccac cgcgatcgcg
     2221 agcgccggga cggccgggag gagggccggc gccgctgcct cgcgaacgaa gcggccgggg
     2281 cgcacgtcga gcgagcggca ggcggccggc agcagcacca gcaggttcat ggccaccacg
     2341 gggatcgcgg ttcccgcggc gacgccggcc agcccccagc ggcggacgag cagcaggctg
     2401 agacccacgt tgagcagcgc ctccgcgagc gacgagaacg ccaccaggcg gtgacgcccg
     2461 gtcccgagca ggatgttgcc gagcgggccc agcgccacca gcacgacact ggcgatcgcg
     2521 agcgcgtaga gcggcgcgag gctgtcttcg aaagtcggcc ccatccacgc gcggacgagc
     2581 ggcggggcga acgcgagcag gccgatcgtc acggccagcg ccaggccgag ggccagcctc
     2641 gtgccgtgca gcatggtggc ccgaagccgc gcgcgatcgt tctgcgcgcc gaggccgacg
     2701 acgaccggga acagcagccc gttgaactgg ttcgagagct gccgctggta gtcggcgagc
     2761 ctggaggcga ccgcgtagac cgcgaccgcg ctggttccca tgaaggcgcc gacgacgacg
     2821 ttgtcgaggt tgtagcccag gtgcgccgcg acggtgatca ggaagaggta gaggctgtac
     2881 gacgtcacct cgcgcaggtg gcgcggcgag aacagcgagg gcgccaggcg cagccccggg
     2941 taggcgaacc aggcagcgcg cgcgtacgcc acgtaggcga gcaggttgac ggccgtcgtc
     3001 gccggcacca ggacacgcag gccgtaaccg gcctcgagca ccaggtaggt gaccagcgcg
     3061 gccagcaacg acacggcgat ggcgatgctg ctgctcagcg cgaagtactg gcgcgaagtg
     3121 gtcaccgcgc cgaagacggt catcgggaat cccaccgcga tacggatgcc gaggacgacg
     3181 gtcacccact gggcggtcgt cacctggccg gcggagaggt tcgggaagcg cggcacgacc
     3241 caggccgcca gcgcggcggt tgcgagcaag gcgacggcgc cgatcgccgc gtagaccacg
     3301 gcgaaggtac tgaccaactc gttgatggcc cgctcgtccc cgcgggcatc ggcgtcggtg
     3361 atctggcgca ccaggccgtt gccgtagccg aggtcgaaga gctggaagta ggcggtcatc
     3421 gagacgacca gcatccagag cccgtagtcg ctcttgccca ggtgccccac gctgaagggc
     3481 atcatccaga tgccaatccc gatgttgacc gcgagaagga cgtaccgggt cgcggtcccc
     3541 gtggccaggt tgacggcgat ggatcggccg gggcggagcg gcatcatcgt ccctgccagg
     3601 ccccgcggag gtagccgacg aaccagagca gccgcgtgag gctggcgaac cgctcggggg
     3661 cctggcggcc gagcgccgcg cgcccgaacc gggccagctc gaccccggcc tgccgccaca
     3721 agtagcgcgg cacgccgagg aggtaccgtg acgtcgtcgg gaactcgcgc tcgattgccg
     3781 acactgtgcg cccgttgtcg tgcagccagc gcaggaagta cgcgcgccgc agccgctcgg
     3841 ccggcacgaa gtgcgcaaca gaggcgcgcg gctcgtaaac gccccgcatc ccggcgcgaa
     3901 gcatccgcag gtagaactcg tggtcctcgc cgctcctcaa cgtgtggcgg agcttcccca
     3961 ggtcggctcg ccagccgcca atctcccgca cgacctcggc tcgcaccgcc atgttggccc
     4021 cgatcggcat ggcgtgctcg ttgagtccgt ggcggatctc gaagcgctcg ggtccgttgt
     4081 ctggcactgc cagcacgccg taaaggcgcg gcgagatcca gggcgggggc gggatctccc
     4141 accgcggccg gattcgcccg acggcgaagt cggcgccggt ctggtcgatc gcctccacca
     4201 tggcgcgcac ccacccggga tcggggagca cgtcgtcgtc ggtgcagacg aggattcggc
     4261 cgagcgccgc cgcgaccccc tggttcaacg catgcgactt cccgggccgc gcttcgtgaa
     4321 ggagccgcac cgccgggacg tcacggcggg ccgcgaaggc ggccaccatc tccggggtgc
     4381 cgtcgctcga cgcgttgtcc accaccacga tctcgagccg gtcgcgcggc cagtcctggc
     4441 gggcgatggc ctcgagcgtc tcgcccagca gccggcgccg atcgtgagtt gcgacgagca
     4501 cgctgatcac ggtcatcctt ccgagagggt ggcaagggcc cgctcccggg agtgggcgtg
     4561 cgcggccggc tcggcgagcg gggcctccag ctccagcacc tcgtacacgc aagccaccgc
     4621 ccgcgcggtg gccgtccccg gccggtagaa gaaccgcagc gcggtttcga cgcgttcggc
     4681 gtgatgccgc gaggggtctg ccaactgcgc gacgacggcc tccaccgtgt cccgcacgtc
     4741 tgaagtcacc tccgcggccc gccggaggtc ggccaccttc gacttcggca cgcgcgcgtg
     4801 cgccagaagg tcggggcagt cgatcaccac gatggggcgg tcgagcacca tgaactcgaa
     4861 cccgacggag ctgtggtcgg tcaccagggc gtcggcggca gcaaggtacg gcgttgcgtc
     4921 cgcgccggtg gccaggcgga tgcgcgggtg gtcgcggtag ggcgcgaaga ccgccgccca
     4981 atcgacgccc cccgacgccc gttcggcgag atcgagcgag cggtcgtgca gcttgatgat
     5041 cacgttgcac cccgcggcct cgagcgccgg cggcagggag tgccccacac gcttgagaga
     5101 cgaatgcggc gaccaggtag gggcgtacag cacggtgggc ctgccggggt cgaggcccag
     5161 gccggcgagg atggcgccgc catcgagcga tccgtccacc agggcgtcga gcttcgggta
     5221 tccggtcagc tggcccaccg gggatccggg cggtacgagt cccgcctcga cgtaacggtt
     5281 cagccggtcc tcgttcggaa agagcaggcg gctgtaggtc cggaccgtcg gagcgatctc
     5341 gaccggcgcg tcgaggccgt acttgccggc cacgccgtgg aacaggtgga tgcggcgggt
     5401 cctccggtag agccaggtcg agggccagaa gtcggtgttg accaccgcgt ccaccttcag
     5461 ccacacggcc cggcgtgcgg ggacgacgtt gcgcgtgata cccacgtgcg cgtacagctg
     5521 ctcgacgtcc cacgttcgcc cggcggtgaa ccagaactcg aaccgggggt cttcgcgaag
     5581 gcgttcgtaa agggggcgga agatcgcgaa gctcatcggc gacgccgcct cgaacaggat
     5641 ccggcggcgc tccccggcgc gcagccgata gaccgcggtc tcggccacgt gcagcgtgcg
     5701 gacgacggcg cggcgcgtgc ggcggtatgc cgttcggtcc accagcgact cgggaacgct
     5761 ggagtcgcgg tgcggctgtg ccagcgcgtt cggctgcccg agagcggcca gcacgcgccg
     5821 ggcgccggtc cgcgagcgct ctccagcaaa ggtggccgcc agctgcagcc aggcggaggt
     5881 cgacaacggc cgccggccga gtgcgcgcca gaaggcggcg cgtgccgagg cgcggtcccc
     5941 ctcgcgcagc cgcgcgtatc cgagttccca gtagaagcgg taccaccggc gcctcaggca
     6001 tcccgcgggc tctgcgcggt ggagcgagca ccccgatcgg cacgtcgcga cgttcttggc
     6061 gatcgcctcg gcctggccac ggaaggtctt ctcgatgtcg gcgctcatcc cgccgccgag
     6121 caccctcacg gcggccggct gcgcgaggta gccgaccggg tagctggccg cgatgcgcag
     6181 ccagaggtcc cagtcctcga cgtggacctc gcgccgctcg tcgaacaggc cgaccgagtc
     6241 gaggacgtcg cgccgcacca tcaccgtcaa cgtggcgatg acgtagtcgg tgtggaacag
     6301 ctcgcagaag acgtggcgtg gcgggcgcgg gtggctgtcg aagacggcca ccgacgacgc
     6361 gtcgccgggc cgtcccgtcg tggcggtgtg caggaggccg gtctgcgggt aggcatggaa
     6421 gtaggcgacc tgcgcctcga gcttgtgcgg aagccaccgg tcgtcggcgt ccaggaaggc
     6481 gatcagccgg ccccggctcg cgcggatgcc ggtgttgcgg gcgctgcccg ggcctccgtt
     6541 ctcctgccgg atcaagctga tctcgtcgcg gccgatgttc ccgacgacct cggccgtgtc
     6601 gtcggtggag ccgtcgtcca cgacgatcac ctcgtagtcg gtgaacgtct gggcgagaac
     6661 cgaggccagg gcctcaccga tcgaaccggc cgcgttgtag gcggggatga tgacgctgac
     6721 ctgcggcgcg gccggccgat ggtgcggcga ggggggcgcg gccatctcaa ccccgggccc
     6781 acgcggcggg cgcgggcgtc atcggctcgg ccgcccgggc gcggggcgcg gccggcaccc
     6841 tggctgcgat cggccgcgtg aggtcccggg ccacggccgc caggccgatg aggtagtaga
     6901 acgtccagtt gtaggcgatc gacgagaaca gcgagcagac gaaccagccg gtcaggccgg
     6961 ccaccagtgc ggtggtgtgc aggcggagca cacgcacgtc acgctcgacg cccggagatg
     7021 cctgcacgtc gggggcgcgc gccaggcgcg tgagggtcct gcgggtcgag agggcggccc
     7081 ggaagccgac gacaatcagg aacacgaatg ccgtgatgcc gaagatgccg gtttcagccg
     7141 cgacctgcag cagcaggttg tgcgactggc gccaccgctc tttcttccat ggcgggttgt
     7201 agttctggaa ctggccggcg ccgatgccgg tgagcggccg ctcgaggaag acccaccagg
     7261 cctccttcat gaccgtgcgc cgcgcttcgc gcgaaccggt gtcgtccttg gccgggtcgg
     7321 tgatgcccgc catgcggtcc cagaagctgg aggggagcgc cgggatcgcc agcaggccac
     7381 ccgcgatcgc cgcgacggcg aacccgcgcc gcagctggcg gccgtagaac acgaggacga
     7441 cgagcatcgc cagcaggccg aggaaaccgc tgcgcgactt ggtgaagacc acggtcagga
     7501 acatcgcccc cgcgcacccc gcgtacagca gcgaggtgaa ccagccggga tcgcggcgga
     7561 accggcttct ccttccgggc gccgcggcgg cggcctgctg gccggccggg ccgttcggcg
     7621 ccgaatcacc gggagcggag gagggcgccc cccgcctcag gcggctgacc accatggcgg
     7681 tcaggattgg gaagggcagg aacgacacca tgttcatggc caggtcgttc gggttcccga
     7741 agatcccgct gacggcgcct gccacgcggc cgtgctcgat gaggttgacg cccctcaggt
     7801 agtcgaagac cgcgcggacg gagaggtagg cgagcgacag gacgatgagc cgggagaagt
     7861 gctgcagccg gcgtggcgag ttgatcgagt tgatgatcag gatggtgatc agcaccacct
     7921 tcccgtagag gttctggatg acgttgaccg aaccacctgg ccagaacgag aaggggacgg
     7981 tgccgacgag caccgcgccg aaggcgagca gccccacgac ctcgggcgtc aggcggaaga
     8041 ccggcaggcc gcgcgccagg cgcttggcgg ccatggcggt cagccccacg agtgccgaaa
     8101 cctcggcgag gtggagggcc gcgagcgcag ggatctggtc ctgggggcgg aagaacagga
     8161 cggccgtgaa ggcgagcaga ccgatgaagg cccagtcgct gctctcgccc ggcagggggt
     8221 cggcgacgac cgacggcatc tccgactcgc gcagctcgcg cgtttcgaag cgcacctccg
     8281 gcacctcccg ccggccgggc accgcaccgt gtccccaggc gagtcgctcc atgcctaccg
     8341 ccagagcctc cggccgcgga cggccatcac caggcccgcc accgccgcat agttcatcag
     8401 tgcgaaggtg aaggccacct tcgacacccg atcaatccag ctccgcattc accaactcct
     8461 tcccgtgcgc cttcctctgc cccgaggccg cggcggcggc gccgtgcagc gcgaggacgt
     8521 agaaggtcac ctggaccacc aacgcgaggc tgtaaaccgc cccagagctc gccagcgccg
     8581 cgctcgacac gagcagggcg atgagggcat aagggacgac caggcggccc atcttgtgcg
     8641 acacgaactg cacgaagagc gggttcgccc ccgggacgag cagcctcggc tccagccgca
     8701 ccagctggta gttgccggcc agcgttcgca ccttccgcct ccgctcggtg actgcgtcgg
     8761 gcgaggcggt gtcgaaggcg atcgccgacg gctcgaacac gacccggtaa ccgccgagcg
     8821 cgatccgcat cggtgccagc acgtcgtcga gaagcgtgtc gggaggcaac gggcgccaca
     8881 gcgcgcggcg aacggcgtag atcgcgccgg tggcgcccac caccgagccc acggcgctct
     8941 cgcggcgccg gatccacttc tcgtaccgcc agtagaggcc caccccatcg ccgaccgccg
     9001 aggccgcggc ggccccgtcc ccgcgctcgg catcgatcac cagctcgccc gagacggcgc
     9061 cgacctcggg gtcggcgaac cgtgcgacga ggcggcgcac cgcatcgggc gcgaagcgct
     9121 ggcgggcgtc cgcgaacacc acgatctcgt ggctcgcggc gccgacgccc gcgttgaggg
     9181 cggccgcctt gcccgacggc ggcaggcaga tcgaacgcac cgccggggcg tacgacgcga
     9241 gcagcgccgg cgtctcgtcg ctcgagccgt ccgagacgac gacgatctcg cggcggccgg
     9301 cggggtagtc gagcgccagc aggttctcga ttcgcccgcg caggacggcc gcttcgttac
     9361 gcgcggcgat gacgatcgag acgggcggct cgatcccctc cggcggcgaa gcggggcggc
     9421 ggcgcgcagc gcgggcgcgg cgcctggcgg cgagcggcgc ccaggcggcg agtaccagcg
     9481 ggtacccggc atacacatag gccacgatga tcgccgagat ccagaacacc cacttcatgc
     9541 gacaccgccg ggggtcagcg atcctccacg gcgccgaccg gcccgccgag gaatgcgtgc
     9601 ttcacccggt cccagaagcc gcggcgctgc cgcgggtccc agtccccggc atcgggcggg
     9661 acgtacggtc gtgggcgcga agcggcctgg gccgtcgcgc ccacccgagg cgggggctcg
     9721 acgcgcccgc ccggacgggc gggcggccac gtcttcgccg cgcgtcgcgg gtcggccgag
     9781 gcgcgagtca tctcgcgggc ctggcgcgac cggaagaggc ggtactcctc ggcgagaacg
     9841 cgctgctgtt cggcaagctg ccgttcgcgc cgggcgagct gatcctcgcg cgcccgaatc
     9901 tcggcctgct gcgcggccag ctggcgctcg cgggccacct cctcctcgcg ggcggaaggg
     9961 atcgccgcct cctgccgcgg ggacagcgga ggacgcaccg ggccggccgg atcgccaggg
    10021 cgctccgtct ccgggcgcag gacgccggtc gcctccagct cggcgaacac ctcgtccatc
    10081 agcgccgcct cgatggcgcg gcggtcctcg gcgtaggcgt agacgagagt cgcgtcgcag
    10141 atgacgttga tgagacgggg caccccgcgg gcgcgggcgt agacacggtc ggtgacttcc
    10201 cgcgggaact ggagcggcgc gccaagggcc gccctccgga gccggtggtt gatgtagcgt
    10261 tccgtctcgt cggcgtcgag cgcctgcagg tgatagctga ccgtgatccg ctgccggagc
    10321 tgctcgaggc gcggcaggtc cagctgttgg cgcaggtcgg gctggccgac caggacgatc
    10381 tgcagcagct tcgatttttc cgtctcgagg ttcgacagca tccggatctc ctcgagcgcg
    10441 gggagcccga ggttctgcgc ctcgtcgatg acgagcagca ccaggcgccc ggcggcgcgc
    10501 tcctcgacca accggatcga caggtcccgc agcaacgccg gcttgccgga aggcgacggc
    10561 gggatgccca ggtcgatggc gatcgcctcg agcagctccc gcggctcgag catggtgttc
    10621 atcagccgta cgacggtggt ctggccgtcg agacctcgca gcagcgcctg cagaagggtc
    10681 gttttccccg acccgatctc ccccgtgatg agcacgaacc ccgcctgggt ctcgatgccg
    10741 tagcgcaggt agtcgagcgc ctcgcggtgg acccggctcg gatagaggta gtcggggtcg
    10801 gggctcagcg cgaacgggcg ctcgcgaagc tggaagtagc tctcatacat gacctggctg
    10861 cggcctccat tccgcctcca tcggttccgg cgccagcgtc ggcacggccg gcgtggccgc
    10921 ggccgagtcg aaggtcgcct cgatcgaggg gagaacctcc tgcgaggccc tcgcgtagtc
    10981 ctccgggaag tcgatctcga tccaggggaa gccgcgggtg ccgatcgcat ggagcgggcg
    11041 gaccctggca aacgcctgga aggcgcgcgg cgcccactcg cgaacccctc cgcgcgagac
    11101 gatcgcgtcc atctcggcga cgagctggcg ggcgccggcg gggccgaact tggcgatccc
    11161 gacgttctcg ccgtcggcgt gctcggcggg cagcgtcttg gcgatgtcgg ccacgcgccc
    11221 gccgcggacc tgcaccttca tctcctcgac gccgaacggc tcgagcagcg agtcgcggaa
    11281 cgcgacgagg agcgcgtcct cgacccgcga gcacacgagg tcgcggagca gttgcgggtg
    11341 gaacagcacg tcgccgttca ggacgaggaa gccgtcggtc agcaggtcgc gcgtgagcca
    11401 cagcgagaag aggctgttcg tctcggcgta ccgcccgttc tcgaccacgc gtgccgtggt
    11461 gccgcaggcc tccgcgaccc gcggttcctg gtagcccgcc actaccgtga tatctgagat
    11521 cccgcaggct cgcagcgcgg ccagctgccg gtgcagcagg gtggcgctcc ccacgcgcag
    11581 caggcacttg ggacagtccc cgccggcacc gtgcagccgg cggccgctgc cggccgcgag
    11641 gatgatcgcg cgcggcgcca ggcccttcgc gcgcatcact tcgccccctt cccgaacagg
    11701 atcactttca cggtgtccac caggatcgtc acgtcgaacc cgatcgagag gtgcttgatg
    11761 tagtagaggt cgtaacgcag cttctcgagc gcatcctcca ccgacgcccc gtagcggtac
    11821 ttgacctggg cccagccggt gatgcccggg cgcaccgcgt ggcgctgctc gtagaacggg
    11881 atctgcgccg ccagctgcgc cacgaagaat ggccgctcgg gtcgcggccc cacgaagctc
    11941 atgtcgccgc gcagcacgtt ccagagctgc ggcagctcgt cgaggcgggt cgtgcgcagc
    12001 gcgcgcccga cggcggtgac gcgcgggtcg gcgtcggcgg cccacacggg cgtctcgccc
    12061 tccgcgtcgt ggcgcatcga ccggaacttg tagagcgtga aagggcgccc gtttcggccc
    12121 gcgcgttgct gccggtagat gaccgggaac ccatcctcga tccagatcgc ctgggcggcc
    12181 agcaccagga ggggcgaggc gatcaccagc ccggcgccgg cgagcgccag gtcgaacgcg
    12241 cgcttgacga ggcgggtcca ggccgacacc cggaacccct ccgagaagat cagccagctg
    12301 ggacgcaggt tgtccaccag cagcttgccc gtgatccgtt cgtaggtggc cgtggcgtct
    12361 tcgacgcgga tgcccgagag cttcgcctgc atcagctcct gcaccggcag ccggccccgc
    12421 cggtcggcga ggcccaccac gatgcggtcg atggcgtact gctcgacgag gcgcgggatg
    12481 tcggtggtgt tgccgagcag ccagccctcg cgatcgcccg cccccgccgg ctcgtcgtcc
    12541 acgaacccca ccacgcggta ggggaagtcc cgctgcgctg cgatctgacg ggcgaccgtc
    12601 cgggcggcgc cgccggtacc gacgaagagg agacgctcct cgagcgcctc gacacccgcc
    12661 acccagaaga gcaggaggcg ccagccgagg acggcaacca ggaagagcag cagcgaggtg
    12721 agaatgaccc ggctcccgag gtcgagcgcc ggcacgaggg cgtagaggcc ggccaggacg
    12781 atggccgccg cgccggctcc ctgcagcagg cgcacgatca gctcgcggct gctgtgcacg
    12841 agggtcaggt cgtagaagtc gttgtagtag agaacgagct gccagagtgc ggccgtggcg
    12901 gccgccttcc accagagacc gtcggccgag gccgcccggt cgttgaggtg gagggcgatc
    12961 gcgatcgagc cggcgatcag gaacacctca cccgcgaagg ccgcagcgcc gcgcgaggag
    13021 atggatcgtc tgaacagtgt cacgtcgatg tcctcaggcc gactgccttc gggccgggaa
    13081 ccggagccgg tgcgtggccg aaccgtccgg gcggtagccg gggtactcga gcgcgagatc
    13141 gtcgacgtcg ttcaacacca gcccgagaac gggcgcgggg tgcacctcgc gaagcgcgcg
    13201 atcgatgttc ggccggctcg tgcgccccgc gcggacgacc atcagcacgg cgtcggccac
    13261 cggcgcgagg acgcccacgt cggccagggc gaccacgggc ggcgtgtcga gcacgatgcg
    13321 gtcgtactgg gtacggagca ggtcgagagt ccggcgcatc tcggccgagc cgagcatctc
    13381 ggccggcagg ccggtcggcc cgcccgccgg cagaacggtc aggtggtagg cgggcagcga
    13441 caccagcgcc tcctcgagcg tggcctcccc ggtgagcacc tcgcacagcc ccggggacga
    13501 gggcacgttg aggaagcggt gcagccggcc cttccgcagg tccgtatcga cgatcagcac
    13561 tcgccggtgg aactcctggg ccatcgtcag ggccaggttc acggcggtga ccgtcttgcc
    13621 gtcccgcgcg cccgggctcg tgacgagcag cgtgcggtac tggcggccat tctcggtgtg
    13681 ggcgatccgg gtgcgcaggg cgcggtactg ctcggcggcc ggcgactcgg gagccagcgc
    13741 cgcgaccagc agggagttga tcgcgtcggg gtcggccgtg cggaccttcg cgaccagcgg
    13801 gttgggcacg acggtcgggg cgggcggcgg cgactgcggc gccggcggcg gaagagcgga
    13861 cgcgcgttcc tgcgaggccg gcctggcggc ggcctggccg gccaccagac gttcggccgg
    13921 cgccaccggc gcggtgcgcc tgacgaaccc gtcgcgctgc gccttgtcga gcacccgttg
    13981 gatgtggctc atgtcgtgtc tcctgcgtgc gcggacctag gccgcccgga tgcgcgagat
    14041 ctcgccgagc accggcagct cgtactcgcg cgccagcgtc accgagtcgt ggaccgagcg
    14101 gtcgaggaac tcgcggccca cgacgccgag gccgccaagg aacgctcccg ccaggagtgc
    14161 tcccagcaga agccgcatgc ggtcgggctt gaacggggca cccggccacg acgcgggcgc
    14221 gaacacccgg aactgctcgc tcgcctggcg gcgctcgaga ctctcggtca tgcgcgccgc
    14281 ctcgcggcgc gcgaccaggt cctcgtactg cttcttctgc acgtcgtact cgcgctgcag
    14341 cgcgagcatc tgctgctcga ccatgggggc cgactccacc ctcgtctggt agaggccgat
    14401 ctgcgcccga gcctgcgact cggcccgctg caactcccgg acccgaagtc ggccgttttc
    14461 gcggtcggcg aggagctgcc ggtaggcggg gtcgttcttg aggtgcgcca ggcggtcttc
    14521 cgcggggcgt tcgcgctcgg cggccgccgt cgcccgggcc gccttgagct cgtccacgag
    14581 ccgaagcact tcggggtgct tttcggtgta gctcatgcgc gcggcggcga gctgctgctc
    14641 gagcgcctgc acgcgcagct cgggcgtggc gggagtggtc gagccgttga gcagcggcag
    14701 cgtctcgact ccctgctgca tcgcctcgat ctgccgctcg atcaccgaga ggcgatcctg
    14761 ctcactgcgg agggccatgt tggtggcctc gagctgttgg cgaaggccgt tgaggctctg
    14821 caggttggcg tcggtctgct cgggcagccg gcccatgttc gcctgcttcg cctcgcggag
    14881 cttcgcctcc gtgtcgtcga gccgctgctg cgcctggcga agctgggcgt cgaggaagac
    14941 ggtggtgtcc tcggcgcgcg cggttcgcga gcgcgaggtc tcgtccacga acacctcgac
    15001 caggcggttg gtgatcctct gggccgcctc cggtgagcca tcgacgtacg acacgtagaa
    15061 caggtcgagt gacggctgcc cattggcggt cggagcgacg ggcgtgggga acgagatcgg
    15121 ttggacgccg cccctcaggc ggccaatcgc cccatcgagc cccacctcgc tggcgtcgag
    15181 gccctcggcg cgcacgacgc gggccagcac ctgccggctg aggagctgct gcgacagcgc
    15241 cctgatgcgg tcgtcgcggt tcagcgccgc cgacgccgcc aggtcgcccg agaccgcggg
    15301 ggcggcgatc ccgacggtgg ccgtggagcg gtactgcttc ggcaggaaca gcaccagccc
    15361 gatcccgacg agcaccgcca gggtggcggg aacgatgaac caccacttcc ggcggcgcac
    15421 gacggagagg tagtcgagcg ggtgaaagcg tgcttcttcc atcacttgat cctcatgggc
    15481 ttcgacgtga ccacctggac cccgagtcgg tcgcggtgaa cctttccccc gggccgcagg
    15541 ctgtcctgct ggcttctggc gtagaaggcc tcgagccgca cgagccgatg caccgcgtag
    15601 ccgacggagg tctggagcca gagcgcgtgc cgggtcagct cctcggcgag cagcgggtcg
    15661 tgccgccgcc aggagaggcc cgcctgccag tagaggcggt tccgggccac cggcatcgcg
    15721 agcacgacgt cgacctgctc gttctggatg ctgccgccga ggccgaaagc agggacgaac
    15781 gagcgcgacc aggcgccgct cagcgtcgcc cgccggaagc gctgcacggc cgagacgttc
    15841 caggcgggcc cggttcgcgc ggtgttgcgg ttggtggcgc ccacgtgcga gacgccgagc
    15901 gaggcggaga gcgagagcgt gcgcgtcgcg tcgtaggcca cggtgccgaa gccctcgacg
    15961 atcccgacgc cctgcccgaa ccgggccatc agcgtgcgct ggtacgacgc gctgccgccg
    16021 agcgaccagc gtgctcccat ctgccgctgg acgccgccgc cgaaggcgtg cgccgtgccg
    16081 ccgcgcagca gcccggcgta cggcgatcgc tcgtcgaagg caacccaggt gaagtcgtag
    16141 ctggcggtgg ccttggtgcg ggcgtcgagc gccacgctca gcgaggcccc cgcgtcgttc
    16201 atccgcgagc cggtgcgcac gaacgggaga tcgctcaaca tgatggcgtc ggtcgtcggc
    16261 accgccgcga agctgtcgtg gaggttcagc gtcacgcgct tcgacagcag gtgacgggtg
    16321 tccacgcgca ggtactggtc gaaggtgttc aatccctcca gctggtggta gagggagaac
    16381 gatcccgtgt accccgcgcc gaaccagtgc ttcttcgcga ggtaggccgc gttgatcgac
    16441 ggcgagatca cgctcagcga gccgttcgtt tccggctcgt ccacgcccac catcgacacg
    16501 ttgctgtccc agccggagga gagcgcgacc gacggggtca gcgtccaccc cggcgtctgg
    16561 cccggctcgc ggacctcctg cagcgggaac tgcgcccagg cgggcggcgc ggcgagcacg
    16621 agcgcggcga gaacgggcgc ggcgaacctc ggagccaccc tcatgggatg atcaccgtgt
    16681 cgcccgcgcg caggtagatg ggcgtcccgt tgccgcgaat cgcctcgcgg tagttgaact
    16741 tgatggtctc gacgccagtg cggtggcgcc gcaggatccg gatgtccttc gtgttcgcga
    16801 agtccttgaa cccgcccgcc atggcgatcg cctgcagggc cgtcatcggc ccctggagcg
    16861 ggatgggacc cggcttggcc acctcgccca tgacgtagat gttcgaggcg agcgcctcga
    16921 cgacgatgac ggtgaccacc gggttggtga cgtactcccg cagcgcgtcg gcgatccggt
    16981 cgcgcagctc gatcggcgtc agctcgaccg ccggaaggtc gccgatgagc ggcatcgtga
    17041 tcttcccgtc ggggcgcacc tgcagcgatt gcgacagctg cggctccttg tagacctcga
    17101 tgcgaagctt gtcgcccgcg ccgaggcggt agtcggacga ggtggcggcg gagcgggagg
    17161 gcgcggccgg agccggggac gccgaggcct gtgggggagg cgcggccggc ttcgagggac
    17221 gctgttcggc cgcgctggcc gtcccggcgg ccagcaccag ggcgttcagc atcagggcgg
    17281 tcagtgcagg tgatctcttc ataggctcaa ttccgtcggt aatgcgggca gaagccgcac
    17341 ttggagcatg agtgcacacc tgagccgcag ctacttcgag caaggctggt gccacgagct
    17401 cagcgcggga gcggagcgag gagggcccgg aacggggacg gccggggcgg cgcggcctcg
    17461 gcgcggcgac ggaagagcag gactttgccg agaaagtgaa ggagcttacc gctggctgat
    17521 gccgcactcc ttgatcttgt tgaggagggt cttgtagctc acgcccagca gctgcgccgc
    17581 cttccggcgg ttccagcgca cctggtcgag cgtctgctcg agggccgcgc gctcggcttt
    17641 catcgccgcg gcgcgggcga tgtcggcgag cgtccgcggc tcctcgggcg gcggggccgc
    17701 cgcgggagct ggctcgagtt cgtctccctc ggccagcggc tcgggctgcg gtgggacggc
    17761 agccgccggg ggcacggact gggccggctg gtacgcaggc ggcccggcgc cggcacctgc
    17821 gccgaccgac atggcgagcg ccggggcgta ggcgggagtg cgcgaggcgg ccgccgtcag
    17881 ctcgcggacg atcatctgct cgtcctggag gatgatcagc cgcttgatca tgttctccag
    17941 ctcccggatg ttgccgggcc agtcgtgcga gaggaagagc tgccggacct cgtcggagag
    18001 cgtcggggcg ggccggttgt acttcttcga gtaccgcgcg atgaagaagt cggtgaggtg
    18061 cggaatctcg tcgcgccgct cccgcagggc tggcaccgtc acctcgatga ccttcaggcg
    18121 gtagtagagg tcctcgcgga agtcgccggc gaccatcatc gcctcgaggt cgcggttggt
    18181 ggcggccagc acgcggacgt ccacggtctg gcgcttgttc cccccgaggc gggtgaattc
    18241 gccgtcctgg agcacgtgga ggagcttggc ctgcagggcg gccttcatct cggcgatctc
    18301 gtcgagcatc agcgtgccgc cgtcggcctg ctcgaacttg ccgatccgtg tgttggcggc
    18361 gccggtgaac gcccccttct cgtgcccgaa caactcgctc tcgagcagct cggtggggag
    18421 ggcggcgcag ttcaccttca ccagcggctt gttgcgtcgc ggcgaccgct ggtggatcgc
    18481 ccgcgccacg agctccttgc cgacgccgct ctcgccccgg atgagcaccg tcacgtcgct
    18541 gtcggcgacg tgctcgatca cgttggcgat gtggcgcatc tggtcgctgt tgccccagaa
    18601 gaggaatgcc cggtcctggt cgtcgctcag ctgctgtcgc agctctgtca gctccgacac
    18661 caggcggttc ttctcgaggg cgttcttgat ggcgacgtcg agcgcgatct cgccgagccc
    18721 ctcggggtcg tcgggcttga cgacgtaatc ggccgcgccg aggcgcacgg cctcgacgat
    18781 ggtcgaggcc tgctcgcgcc cggagagcat gatggcctgc gtctcggggc gcgccccctt
    18841 gagcgcgcgc agcgtcgcca ggccgtcgag cccgggcatc atgacgtcga gcaggacgac
    18901 gtcgggagga tcggcctgcc ggatcgccgc cagcagttcg tcgccccgcg agtacgtgcg
    18961 cgcttcgtag ccgcgaagcg acaggaaggt ccgcaggtag ttcgagaagg ccgaatcgtc
    19021 gtccacgatt gccacgaaag gtttcttcac ccggcgttct ccgcgcgcgc cacggccgct
    19081 ggcagggtca acaggatcga gaggcgtcca cgttcggcga caggggaagc gagccgtccc
    19141 ccgtgcgcct cgatgatacg tgcggccgcc ggcagtcgga aaccgaggcc gccgcggtac
    19201 tcgtcgaagc gttgctgccg tgcctcgacg tcgtctagca ggccggcggc cgtctcggcg
    19261 tccccgaagg cgacgagcgc cgacgggggc tcgccggcga ggcgcgccac cgcgaccact
    19321 cgggcgccct gcaggcgctc gcgcagcaca gccgccatca acgacgcaat cgcctcgctc
    19381 aagcgcgggc gatccgcctc gaccacgagg tccgctctcg cgtcgacctc gacagtgagg
    19441 ccgcgatctc taccttcttg cacgttcgag gccacctgtg cgagcagcgc gccgagcggc
    19501 agcgcctcgc gcgtgagccc gagcgtgccc tgctcgagcc gcgacaggtc cgagaagtcg
    19561 gcgagcagct gcgacagcgt tccgcaggcc ttttcgctct cctcgagcaa tttccgctgc
    19621 tgatccgtga gcccttcgcc gaagtgccgg agcaccatcc gcaggtaacc cgacacgacg
    19681 gcggtcgggg tacggaactc gtggacggcg agcgacagca gccgcgcgca gtggtcgcgt
    19741 tggtcggtca tacagtgatg tatccggagc gatgcccagc cacatcctac catgcaggca
    19801 cggggaggga acggcgttgc cctctcgcgc ggcggaaacc cggcgtcccg aatgaatcgg
    19861 tcggcacagg gcggtgctgc tgtggcacaa cccttgaaca ccctcgcggc gttggaggac
    19921 agctcgtcgt ttcgtttcgg aggagtatcc tggtgacttt tacgagagct tcgagagcgg
    19981 ccctggcggc gcttgccctc gtggtcctgc tggccggcac ctcgatcgcg gtcgccgggt
    20041 ggatgcctgg cgcggggagc cagccgcaat acaccgtcgc atcgaaggag atggacacga
    20101 tcggaacggg aaccgtcgtg gcggcgccca cctcgcgcct gaagggaatc tccgagaagc
    20161 tgatgctggt tgcggtcggc gggctgctgc tcggcctggc cgccgccgtg cggcggaccg
    20221 cgtgagctga ccaagccgcc gcggtcgtct tctcaggcgg tcccttcgcg tgcagaat
//
LOCUS       DAOPQX010000022        20216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3281_length_20216_cov_4.707455, whole genome shotgun sequence.
ACCESSION   DAOPQX010000022 DAOPQX010000000
VERSION     DAOPQX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 20216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20216
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_20216_cov_4.707455"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="PKK95_02335"
     CDS             complement(<1..1554)
                     /locus_tag="PKK95_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOC17072.1"
                     /translation="MKRLVLAAAILAATFGPQAAQDRDIERWERQARAVTIVRDDWGI
                     PHVRGKTDADAVFGVIYAQAEDDFNRVETNYLNALGRLAEAEGESAIFRDLRMKLFVD
                     PDQLKALHAECPAWLRALMQAWADGLNYYLHTHPEVKPRVITRFEPWMALSFTEGSIG
                     GDIERINLSRLEAFYGQEGAARPAPGSGGSAAVGAQVPANDFARQEPSGSNGIAIAPS
                     NTAEGRALLLINPHTSFFFRAEAQMTSAEGLDAYGAITWGQFFVYQGFNDRAGWMHTS
                     SSVDNIDEYLETIVKKGDRLFYRYGPSERPLESRTISIPYRTASGFSSRAFTVFRTHR
                     GPIVREAEGKWVSVRLMEEPVKALVQSFTRTKARSLEAFRDTMALHTNSSNNTIYADA
                     DGNIAYFHANFVPVRDPRADWTRPVDGSNPANDWRGVHAVDETPLVLNPSTGWLYNTN
                     NAPWSAAGPASPRKESYPRYVDAGGENPRGLHAIRVLEGRKDFTLDSLLAAAYDSYLP
                     AFEKLLPALL"
     gene            complement(1577..3640)
                     /locus_tag="PKK95_02340"
     CDS             complement(1577..3640)
                     /locus_tag="PKK95_02340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC17073.1"
                     /translation="MLPKRLPTPLLLILCALVALPARAQVQAGKAAGPATKPPTIDEL
                     IELKSVGSVRISPDGACVAYTVSSTDWKQDAYVTQIWLADVKSGRILQLTRGEKPAGN
                     PAWSPDGAWLGFTSTRAGEKNQVFAIRPDGGEAVQLTNAENGVGGYEWSPDGKMIAFT
                     SSDRDDKAVKARRDRLGDFEVVRREYAHQHLWTIDVAEAMLAPAAGTARTKGRDFSVG
                     SFAWSPDSKTIAFSATVNPDLINGATADIYLLGLDGNQVRKLVGQPGPDTNPRWSPGG
                     SSLVFSSAMGNPKHFHANSRLAVVPAAGGTPRSITDAFDERPGLMDWMPEGVYFAAYQ
                     RTAVHLFRVDPATGRVTRISQPDAPMLTGASFTKDGRTLGFAASSPTTLSEVYVSALE
                     PFAPRRLTSMTEQAKGWTLGRREVISWRSKDGTEIEGVLIKPADFDPAKKRPLLCIIH
                     GGPTGIDLPTMIDTRIYPSDIWAARGALVLKINYRGSAGYGEKFRQLNVRNLGVGDAW
                     DVLSGVEHLVKLGWVDPAKVGCMGWSQGGYISAFLTTSSTAFAAVSVGAGISNWATYY
                     YNTDITPFTIQYLGADPAADPEIYAKTSPMTYIKQAKTPTLIQHGELDRRVPIPNAYE
                     LRQGLEDRGVKVEMIVYKGFGHGITKPKAMRAATGHNLAWFNHYIFGDPLPDLTAPED
                     AVDGK"
     gene            3779..5917
                     /locus_tag="PKK95_02345"
     CDS             3779..5917
                     /locus_tag="PKK95_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC17074.1"
                     /translation="MNERRPRQGGWPTRAERLRLVLFPVLLAPAQLLLFGPFTTYSRN
                     ETEFAVGFWSLAPSWLPVAAGLSALLVAAGVWLLPRRALRSYVAVLFAVGLLLWVQGN
                     LLVPDLGPLYGAPPDFESVAWRTPYELGVWAAVLVLAVVFARRLSGVAPVASQALLAL
                     QLVVLLAGTTGLLGRSRDEGAPEVKRWSEPPARLFELSRQTNVIHLVLDGFASEIFAE
                     AMQRDRAAFDRAFPGFVYFEDHLGAFRTTRASMPAMMTGVAYRNEVPFDDFSDRVWQE
                     RSIFRVLADHGYQIHSATFHTGEQPPASSRPVRYDLPTPYADRETYERFVSAQVLDLT
                     LFRHAPTALKRRIYNEGRWVLQQVTGLAKRQVRHSSHLLFLDHFTSRLTVALDEPVYL
                     FMHVLPPHPPVVLDASCAPLDGQRFTWASYSGQGACVLTYVQRLLDRLRALGVYDRSA
                     ILLTADHGFSPRRRHHPLEDVITPAGRLYQIATAAAPLLAVKPPGAEGPLRTSQAPTS
                     ITDLPATIATLAGVPAASLPGRSIFEIDAGAARPRTFTFHSWNNADWKRPYFDVLLLF
                     DVNGRVLDAASWSFKQAILDPTNPSDDATQRLEFGLSGVRRENGEAFRWGARYAVTYL
                     PASARTLRVTARKDAATTSRAAVTVRLDGRVAGRLELKRDGWHTWTHRLEPRKSGHLP
                     FCVELLSERDGNRPGVRAGRIQYGPSFWND"
     gene            6039..8162
                     /locus_tag="PKK95_02350"
     CDS             6039..8162
                     /locus_tag="PKK95_02350"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOC17075.1"
                     /translation="MPRRVTAVLAILLALLLRPATGDEGMYPITELSKLNLKAKGLQI
                     PVSEIYNPQGGGTSLIEAIVHLGGCSASFVSPDGLIITNHHCVFGAVSAASSVEHDYL
                     THGFLAPTRGDEIPARGMTARITEEMRDVSSQVLAAVQPGMDPLARAKAIDGRIAELT
                     KEAQAKLPGKTISISEMLPGQSYLMFISTTYRDVRLVYVPPRSIGEFGGEDDNWVWPR
                     HTGDFSFVRAYADKDGRPADHSATNVPFHPRRFLKVNPNGVNEEDFVFILGYPGRTYR
                     HQSSYYLAYEENHRMPWVADLNQWQIQTLEELGKGDRAIALKFDSRIKGLANTMKNYR
                     GKLDGMRKLGLTAARRNEEAALEKFIEADPGRRAAYGTVLADTARIYDGMAARFQYEM
                     VLDNLVSMSALLRAASNVVAAGAGEPAAAPPGRGASNPFATYHEPAEVLFLRKIAAMA
                     GALPEGQRIAPIDQRLGGDFSEENVRRHVEAAVAAATIKSQAAMTAAMKEGPDAVARS
                     TDPFIALARDLAPLQQELRDVRQRRTAELNRLSALLLDARQQYLKKDFVPDANSTLRF
                     TYGRIRGYSPADAVVHKPHTTLDGVIEKTRDQAPYDTPQVVRELAKARDFGRYRLPKT
                     NQLPVGLLYNLDTTGGNSGSALMNARGELVGVNFDRAWAATINDYAWNEAYSRSIAVD
                     IRYVLWVTEKVGKAGFLLKEMGIGD"
     gene            complement(8324..9079)
                     /locus_tag="PKK95_02355"
     CDS             complement(8324..9079)
                     /locus_tag="PKK95_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17076.1"
                     /translation="MRRAAMSLLVVLAGAWFAAGAAQAQTADEVVEKHLAAIGGRAAL
                     QKLQNRVATGTISVTVQGTPLSGAVEVYSAVPNKSRMSFRMDLSAMGAGEIVVDQRCD
                     GKTGWASNSFQGDRDLAGSQLQTMLNEYFPTPLLGYKERGAKIELLGKETVGTRPALV
                     ILFTPTSGFPTKYYFDAETFLDLQQVTRQDVPEAGGEVESRVLADDFRTVDGVKIPFA
                     IKIANSMQDVSITLDKVEHNKAIDEAMFSKPTK"
     gene            9363..10781
                     /locus_tag="PKK95_02360"
     CDS             9363..10781
                     /locus_tag="PKK95_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17077.1"
                     /translation="MDEQNKGRSVISRRGLLGGFAAAAGVMIVPRRVLGGPGYQAPSD
                     TLNIATVGYAHGMGTANTNNCGKENIVALCDVDESARAVRALERSKILEKFPNAKRYK
                     DFRVMLDQQKDIDAVIVATPDHSHAVVAMAAMQLGKHVYVQKPLTRTISEARALTEAA
                     RKYKVVTQMGNQGHSGEGVRLIQEWIEDGAIGKVREVHCWTNRPIWPQGMPAPAEGQP
                     VPEGLDWDLFLGPAPERPYHSAYHPFYWRSWMDYGAGALGDMACHVMDAAYTVLKLGY
                     PTSVQTTVAYIVQPVTRPDGSPGSRRVEYKDSFPPAEIVRYTFPARSKDLPEVKLTWY
                     DGGLLPERPAELEPDRRLPESGTIFIGDKGKLMSETYSEGPRLIPESRMQAYKRPKKT
                     LARVPGTHEENWIDAIKGKTPKPTSNFDYSGPFTEAVLLGNLGVMFPGQKLLWDGPNM
                     KVTNVPEANEYVQHHYRQGWSL"
     gene            10911..12173
                     /locus_tag="PKK95_02365"
     CDS             10911..12173
                     /locus_tag="PKK95_02365"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17078.1"
                     /translation="MHKTNRRRFLKQATAATAGFMIVPRHVLGGTGFIAPSDQLTKAI
                     IGVGGMGRNHIPYEGTRVLAVCDVDRTHLEKALSMSGPDAKGYSDYREVLQRPDIDVV
                     HVATPPHWHGLIAIEAARAGKDIWCEKPMTRTIGEGLRVIDEVNRHGRMFRLNTWFRF
                     KDTFYGLGTDVKPLKKLIQNRVFGWPLTVTVSGVTGYDWKFYWSGRTDLTPQQVPPEL
                     DYEMWLGPAPYKPYHPHRVHANFRGYWDYDGGGLGDMGQHYLDPVQYMLEKDETSPIR
                     VDVDAPQQHYDAAGTWRRIEYTYADGCKIILDGENRDTEAAYISGPKGKLFRGFRSDI
                     PNIEKIVASLPDPEPQVGTFSDSVRTRRKFALNEMNGHRSCTLVNLGILAVRLGRTLH
                     FDPEAQRFRDDEAANRLIDQPMRAPWII"
     gene            12249..15668
                     /locus_tag="PKK95_02370"
     CDS             12249..15668
                     /locus_tag="PKK95_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC17079.1"
                     /translation="MKNARWTCARTALASLVLLAVLAPAPAWAQDNRTLATKVADILA
                     LFPAGSGEDRDKLADQMIALGEPGLAEFTRRLAPAGSGDDTAVRYAVNAMAVHAARAG
                     NEPKRAVAERALVTALGSATDVEVKTFLLSQLRVVGREAAVKAASPLLADAATVEPAT
                     QLLLTVKGPLARTALAAALDKAQGPARITIVKAIGELGAVEANQKLLAFAADPDPAMR
                     RPALAALARLASPRSFDALTGAATKAGFKYEPGNAIGSLLNYAARLGQTGNASTAEKV
                     CRLVMRNTDAPGLLGTRAAALAILADVRGPRALPELIAAVDHADVSYRHAALARAEKI
                     RSAVAVNQWVAKAEKVDPVRRAEIVAMLGRQGDRRSLPFIRASLASADPAVALAAAEG
                     LALMEGPAANEALVGLLKSRSAADAQHVADILLWTTDAKALDPLVPLLQSGEPAQKAA
                     AVRVLGARGGSRFAAQVLPLASDANPEVRAAAYGALAGVAGPGDLPALLKLVDAAPDA
                     QLARNAQRAVVASAEQVTPEEARARPIIAAMDGAVHPERLLEVLPQVGGKEALDAVVS
                     RFEGGSGDTKGAAFRALVQWRGPEAADRLFAIVSAGEPAYRDMAFGGFVRQIGMSSLP
                     DEQKVLQLRRVLPLAKSANERRMIIRSLQRTKTFQAFLVASSLMDDAEVANDAAGAVM
                     TIALPAAGEKNGLTGKLVRNALERVIQVLTGGESDYDKENIKRYLAAMPPDEGFVPIF
                     NGTDLTGWQGLVENPIVRAKMSPQDLAARQPAADAKMRENWGVRSGMIVFNGSGDNLC
                     TVKEYGDFEMLVDWRITKDGDSGIYLRGTPQVQIWDPARVDVGAQVGSGGLYNNQKNP
                     SKPLVFADNPVGEWNTFRIVMIGEKVTVFLNGVKVVDNVTMENYWDRSQPIFARGPIE
                     LQAHGTDLQFRDIYVREIGAEASQGITPEEKAEGFVALFDGTNLDQWIGNKTGYKVEN
                     GMLVFDPKAPDTKNIYTAKEYGDFVFRFEFQLTPGANSGVGIRTPPEGDAAYVGMEIQ
                     ILDNDAPVYAKLQPYQYHGSVYGIIPAKRGALKPVGEWNTEEIFIQGSKIRITVNGQV
                     IVDGDLVEATKNGTMDHKDHPGLQRKTGHIGFLSHGAVVKFRNIRIKDLTK"
     gene            complement(15810..>16981)
                     /locus_tag="PKK95_02375"
     CDS             complement(15810..>16981)
                     /locus_tag="PKK95_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17080.1"
                     /translation="VRGIEVTGVSAAAGAPELASLAREWMVGDAVVYNSVAEMARHVD
                     VIAVFAPNFARISIVEAIVEAVKAGAALKGVICEKPLGRTVAEARRLVDLAASVNLRT
                     AYFENQIFMKPITAQLAQLEPVQRRMGPLSLSRSSEEHGGPHEAWFWDPVRQGGGVLN
                     DMGCHSIAVGRHVLTPIGKPVTFLEPVEVNAEVALLKWGQREWREKLLRDRNVDYAKA
                     PAEDFATGVVTYRNPETGQRVKAQFTNSWMFEKQGLRLFMDGTGPGYAFEVNTLVSPL
                     SIFIGDAAAEAVKDAELALEKSTASRGLLAVQVNEADLYGYTDENAEARDAFGAGRDG
                     FLNWGYGLEVTRLVMAAYLSAERKRVIDLTDPATQKELETYVPLIAQGRGADQLY"
     assembly_gap    16982..16991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17178..18359)
                     /locus_tag="PKK95_02380"
     CDS             complement(17178..18359)
                     /locus_tag="PKK95_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC17081.1"
                     /translation="MQASETRNTSIVALMAVFGLAICFVIVGAISVEYQAALNIENIG
                     NLTFALFLTSAIVQLVIGPSVDRFGYKPVALVGFAVAALSMILLRVAGSYEMAILACI
                     LTGVGAMCLNTVGNTLIPVVLFGGKDPARASNFGNGFFGLGLVVTPLVFSLVNYRAGL
                     VLLAALFLIGFVMAMGTRFPMVSTGYQFSEAFALLRHPAVIVAALALFCYIALESSMT
                     TWSKPYMTEILGGAANPSAARNAGLVLSLFGVAMMIGRFITSAIKGLTKIGVTLIAAA
                     ALVAVGTILVMIVTSSATLASVAVVLTGLVFAPIFPTIVGVTFAKFEPRLYGSIFGII
                     FAVGLLGGTFVPQIVGSLSAGATVQQSLQIVAGMAAALFVIALFLKVGARQAGTPAVT
                     R"
     gene            complement(18421..19293)
                     /locus_tag="PKK95_02385"
     CDS             complement(18421..19293)
                     /locus_tag="PKK95_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC17082.1"
                     /translation="MTNRTRGGGALSRRSFLEVTAAAPLAMAAAARVRADSAIPIALQ
                     LYSLRGDCQADFDACLDAVSRMGFTGVEFAGYFGYAGRGAELRKRLDGLGLKAAGTHI
                     GADQLQGDALKQAIEFHQAIGCRFLVVPSSRDFTDPEKSKALAETFNRAAEQLAPLGM
                     ACGYHNHTAEFKTDGGKTFWDLFAERTKKEVILQQDCGWTFAAGHDPVAYVKKYPGRS
                     RTVHFKPTVREGDAGKKPILALDSVPWRQVYDACRSDGGTEWIVLEQELYPDGKTPAE
                     CVKESFAGLKRVIA"
     gene            19453..>20216
                     /locus_tag="PKK95_02390"
     CDS             19453..>20216
                     /locus_tag="PKK95_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17083.1"
                     /translation="MAVAGAALAPTIVKASALGRGGAVAPSDRIVMAGIGFGMQGPGN
                     MRSFLGKDEVQWVAVCDLDDDHLQAARRTVNEKYGNEDCATYKDYRELYARGDLDAVS
                     IAVPDHWHAILSIEAVRAGLDVFGEKPFTHSLREGRALCDAIRRYGRVWQTGSWQRSE
                     ENFHRAAELVRNGRIGKVLRVEVGLPSGYTDFARTFGQETIEPPPAALDYDRWLGPAP
                     YAPYCKARVHMNWRWNMDYAGGQLMDWVGHHLDIAH"
BASE COUNT         3198 a         7021 c         6901 g         3086 t
ORIGIN      
        1 cagcagcgcc ggcagcagct tctcgaacgc gggcaggtag ctgtcgtagg cggccgcgag
       61 gagcgaatcg agcgtgaagt ccttccggcc ctcgagcacc cggatcgcgt gcaggccgcg
      121 ggggttctcg ccgccggcgt ccacgtagcg cgggtagctc tccttcctcg ggcttgccgg
      181 gcccgccgcc gaccaggggg cgttgttggt gttgtagagc cagccggtcg aggggttcag
      241 caccagcggc gtctcgtcga cggcgtgcac gccgcgccag tcgttggccg ggttgctgcc
      301 gtccaccggg cgcgtccagt cggcgcgggg gtcgcggacg gggacgaagt tcgcgtggaa
      361 gtaggcgatg ttcccgtccg cgtcggcgta gatcgtgttg ttcgacgagt tggtgtgcag
      421 cgccatcgtg tcgcggaacg cctcgagcga gcgggccttc gtccgggtga acgactggac
      481 cagcgccttg acgggctcct ccatcagccg gacgctgacc cacttcccct ccgcctcgcg
      541 cacgatgggg ccgcggtgcg tgcggaagac ggtgaacgcg cgcgacgaaa agcccgacgc
      601 cgtcctgtag ggaatcgaga tcgtccgcga ctccagcggg cgttcggacg ggccgtagcg
      661 gtagaagagc cggtcgccct tcttcacgat cgtctcgagg tactcgtcga tgttgtccac
      721 gctgctcgac gtgtgcatcc agccggcgcg gtcgttgaac ccctggtaga cgaagaactg
      781 accccacgtg attgcgccgt aggcgtcgag cccttcggcg ctcgtcatct gcgcctcggc
      841 gcggaagaag aacgaggtgt gggggttgat caacagcagc gcccggcctt ccgccgtgtt
      901 cgatggcgcg atcgcgatgc cgttggagcc cgacggctcc tgccgggcga agtcgtttgc
      961 cgggacctgc gcaccaaccg ccgccgaacc gccgctgcct ggagctggcc gcgcggcgcc
     1021 ttcctgtccg tagaacgcct cgagccggga caggttgatc cgctcgatgt cgccgccgat
     1081 gcttccctcc gtgaagctga gcgccatcca cggctcgaag cgcgtgatga cccttggctt
     1141 cacctccggg tgggtgtgga ggtagtagtt gaggccgtcg gcccaggcct gcatcagcgc
     1201 ccgcagccac gccgggcact ccgcgtgcaa ggccttcagc tggtcgggat cgacgaacag
     1261 tttcatccgc aggtcgcgga agatcgccga ctcgccctcg gcttcggcca gccgccccag
     1321 cgcgttgagg tagttggtct cgacccggtt gaagtcgtcc tcggcctgcg cgtagatcac
     1381 gccgaagacc gcgtcggcat cggtcttccc ccgcacgtgc gggatgcccc agtcgtcgcg
     1441 aacgatggtg acggcccggg cctgccgctc ccaccgctcg atgtcccggt cttgcgccgc
     1501 ctgcgggccg aacgtcgcgg ccaggatggc ggccgccagt acgagtctct tcatagtcga
     1561 atccctggct gattccctac ttcccgtcca cggcgtcctc cggcgccgtc aggtcgggca
     1621 gcgggtcgcc gaagatgtag tggttgaacc aggccaggtt gtgcccggtt gcggcgcgca
     1681 tcgccttagg cttggtgatc ccgtggccga accccttgta gacgatcatc tcgaccttca
     1741 ccccgcgatc ctcgagcccc tgacgcagtt cgtaggcgtt ggggatcggc acccggcgat
     1801 ccagctcgcc gtgctggatg agcgtcggcg tcttcgcctg cttgatgtag gtcatcgggg
     1861 acgtcttcgc gtagatctcc gggtcggcgg cgggatcggc gccgagatac tggatcgtga
     1921 agggggtgat gtcggtgttg tagtagtagg tcgcccagtt cgagatgccc gcgccgaccg
     1981 agacggccgc gaacgccgtg ctggaggttg tgaggaacgc cgagatgtag ccaccctggc
     2041 tccaccccat gcaccccacc ttcgccgggt ccacccaccc gagcttcacg aggtgctcga
     2101 ctcccgacag cacgtcccac gcgtcgccga cgcccaggtt ccggacgttc agctggcgga
     2161 acttctcccc gtagccggcg ctgccgcggt agttgatctt gaggacgagc gcaccgcgtg
     2221 ccgcccagat gtccgacggg tagattcggg tgtcgatcat ggtggggagg tcgatgccgg
     2281 tcggcccccc gtggatgatg cagagcagcg ggcgcttctt cgcggggtcg aaatcggccg
     2341 gcttgatcag caccccttcg atctcggtgc cgtccttgct gcgccacgag atcacctcgc
     2401 ggcggccgag cgtccatccc ttcgcctgct cggtcatcga cgtcaggcgc cgcggagcga
     2461 acggctcgag cgccgagacg tacacctcgc tcaacgtcgt gggggaggag gcggcgaagc
     2521 cgagcgtccg gccgtccttc gtgaagctcg ccccagtgag catcggggcg tccggctggc
     2581 tgattcgcgt cacccggccc gtagccgggt ccacccggaa caggtggacc gcggtgcgtt
     2641 ggtaggcggc aaagtagacg ccctccggca tccagtccat gaggccgggc cgttcgtcga
     2701 aggcgtcggt gatcgagcgc ggggtgccgc ccgccgccgg cacgaccgcc aggcggctgt
     2761 tggcgtgaaa gtgcttcggg ttacccatcg cggacgagaa aacgaggctg ctgccaccgg
     2821 gtgaccagcg cgggttggtg tccgggccgg gctggccgac gagcttgcgg acctggttgc
     2881 catcgaggcc gagcaggtag atgtcggcgg tcgcgccgtt gatcaggtcg gggttcaccg
     2941 tcgcgctgaa ggcaatcgtc ttcgagtcgg gagaccaggc gaacgagccg acgctgaagt
     3001 cgcgcccctt cgtgcgggcc gtccccgcgg ccggcgcgag catcgcttcg gccacgtcga
     3061 tcgtccacag gtgctggtgc gcgtactctc tgcgcaccac ctcgaagtcg ccgaggcgat
     3121 cgcgtcgcgc cttgaccgcc ttgtcatccc ggtcgctgct ggtgaacgcg atcatcttcc
     3181 cgtcgggcga ccactcgtac ccgccgaccc cgttctccgc gttggtcaac tgcaccgcct
     3241 cgcccccgtc cgggcggatg gcgaacacct ggttcttttc gccggcccgg gtgctcgtga
     3301 agcccagcca tgccccgtcg ggcgaccacg ccgggttgcc cgccggcttc tcgccgcgcg
     3361 tcagctgcag gatgcggccc gacttcacgt cggccagcca gatctgcgtc acgtaggcgt
     3421 cctgcttcca gtcggtcgag ctgaccgtgt aggcgacgca ggcaccgtcg ggggagatgc
     3481 ggacgcttcc cacgctcttc agctcgatca gctcgtcgat cgtgggcggc ttggtggcag
     3541 ggccggccgc cttcccggcc tggacctgcg cccgggccgg cagggcgacg agcgcgcaga
     3601 ggatcagcag gagcggcgtc ggaagacgct ttggcagcat ggtcgttcct cccggatgtc
     3661 gaaggggcgg ggcggagcgg ccagtatagc ggaaggcgcg aagcggagac cgcgaagggc
     3721 ggacgagggc cgaaccggat gccgaagggg gttgacgccg gacgcacaat ggtgaccgat
     3781 gaatgaacga cgcccccggc aagggggttg gccgacacgc gcggagcggt tgcggctcgt
     3841 gttgttcccg gtgctgctcg cgccagccca gctgctcctc ttcggtccct tcaccaccta
     3901 ttcccgaaac gagaccgagt tcgccgtcgg cttctggagc ctggcgccgt cgtggctccc
     3961 ggttgcggcc ggcctgtcgg cgctcctcgt tgcggccggc gtctggctcc tcccgcgccg
     4021 cgcgctgcgg tcctacgtcg cggtcctctt cgcggtcggg ctgctgctct gggtccaggg
     4081 caacctgctg gtgcccgatc tcggtcccct ctacggggcg ccgcccgact tcgagagcgt
     4141 tgcctggcgc acaccgtacg aactgggcgt gtgggccgcc gtgctggtgc tggccgttgt
     4201 cttcgcgcgg cggctgtccg gggtcgcgcc tgtcgcgagc caggcgcttc tcgccctcca
     4261 gctcgtcgtg cttctcgccg ggacgacggg actgctcggg cggtcccgcg acgagggggc
     4321 gccggaggtc aagcggtgga gcgagccccc ggcgcgactc ttcgagctgt cgcggcagac
     4381 caacgtcatc cacctcgtgc tcgacgggtt tgcctcggag atcttcgccg aggcgatgca
     4441 gcgcgatcgg gccgccttcg accgcgcctt ccccgggttc gtctacttcg aggaccatct
     4501 cggggcgttc cggacgacgc gcgccagcat gcccgcgatg atgaccgggg tggcctatcg
     4561 gaacgaggtt cccttcgacg acttcagcga tcgggtgtgg caggagaggt cgatctttcg
     4621 cgtgctcgcg gaccacgggt accagatcca ctcggccact tttcataccg gtgagcagcc
     4681 gcccgcttcc agcaggccgg tccgctacga ccttccgacc ccgtacgccg accgcgagac
     4741 gtacgagcgg ttcgtttccg cgcaggtgct cgacctgacg ctgttccggc acgcgccgac
     4801 cgccctcaag cgccggatct acaacgaggg gcgctgggtg ctccaacagg tcacgggtct
     4861 ggcgaagagg caggtccggc acagcagcca cctgttgttc ctcgaccact tcacctcgcg
     4921 cctgacggtc gccctggacg agcccgtgta cctgttcatg cacgtgctgc cgccgcaccc
     4981 gccggtcgtg ctcgacgcga gctgcgcgcc tctcgacggg cagcggttca cctgggcgtc
     5041 gtactccggg cagggggcgt gcgtgctgac gtacgtgcag cggctgctcg atcggctccg
     5101 tgcgcttggc gtctacgacc gcagcgcaat cctgctgacg gcggatcacg ggttcagtcc
     5161 gcggcggcgc caccacccgc tggaagacgt catcacgccg gcggggcggc tctaccagat
     5221 cgcgaccgcg gcggcgccgc tcctggccgt gaagcctccc ggtgcggaag gaccgcttcg
     5281 gacgtcgcag gcgccgacct cgatcaccga ccttccggcc accatcgcca ccctggccgg
     5341 ggtgccggcg gcgtcgctgc ccggccggtc gatcttcgag atcgacgcgg gcgccgcgcg
     5401 gccgcggacc ttcacgttcc attcctggaa caacgcggac tggaagcggc cgtacttcga
     5461 tgtgctgctg ctgttcgacg tgaacggccg ggtgctggat gcggcttcgt ggagcttcaa
     5521 gcaggcaatc ctggatccca ccaatccctc ggatgacgcc acgcaaaggc tcgagttcgg
     5581 cctttcagga gtgaggcgcg agaacggaga ggcctttcgc tggggtgccc gctacgcggt
     5641 gacctatctc ccggcgagcg cccgcaccct gagggtcacg gccaggaagg acgccgcgac
     5701 gacgagccgg gcggcggtga ccgtgcgcct ggacgggcgc gtcgccgggc ggctcgagct
     5761 gaaaagggac ggctggcaca cgtggaccca tcggctcgag ccccgcaagt ccggtcacct
     5821 gccgttctgc gtcgaactgc tgtccgagcg cgacggcaac cggcccggtg tgcgggccgg
     5881 ccggatccag tacgggccct cgttctggaa cgactagcag gccgctgaaa atcgcggcct
     5941 gaatgcaaaa tgcacaagga agaatgtccg ccggggtcgg ccttcgctcg cgcgttttca
     6001 cgtaagctac acgctgcaac ccgtttcgag gagtcttcat gccgcgacgc gtgactgccg
     6061 ttctcgccat cctgctcgcc ctgctcctcc gcccggcaac gggtgacgag gggatgtacc
     6121 cgatcaccga gctgtcgaag ctgaacctga aggccaaggg gctgcagatc ccggtgtcgg
     6181 agatctacaa cccgcagggg ggcggcacca gcctgatcga ggcgatcgtg cacctcggcg
     6241 gctgcagcgc ctcgttcgtt tcgcccgacg gcctcatcat caccaatcac cactgcgtgt
     6301 tcggggcggt ctcggcggcc agctcggtcg agcacgacta cctgacccat ggcttcctgg
     6361 cccccacgcg cggcgacgag atccccgccc gcggcatgac cgcccgcatc accgaggaga
     6421 tgcgcgacgt gtcctcgcag gtgctcgcag ccgttcagcc cggcatggac ccgctggcgc
     6481 gggccaaggc gatcgacggc cgcatcgccg agctgaccaa ggaggcccag gcgaagctcc
     6541 ccggcaagac catcagcatc tccgagatgc tgccgggcca gtcgtacctg atgttcatct
     6601 cgaccaccta ccgcgacgtg agactggttt acgtcccgcc acggtcgatc ggcgagttcg
     6661 gcggcgagga cgacaactgg gtctggcccc ggcacacggg cgacttctcg ttcgttcgcg
     6721 cctacgccga caaggacggc cgcccggccg atcactcggc caccaacgtc ccgttccacc
     6781 cgcggcgctt cctgaaggtg aacccgaacg gcgtgaacga ggaggacttc gtcttcatcc
     6841 tcggctaccc ggggcgcacc taccgtcacc agagctccta ctacctcgcc tacgaggaga
     6901 accaccggat gccgtgggtg gccgacctca accagtggca gatccagacc ctcgaggagc
     6961 tggggaaggg cgaccgggcg atcgcgctga agttcgactc gcggatcaag ggcctggcga
     7021 acacgatgaa gaactaccgc gggaagctcg atggcatgcg caagctgggg ctgaccgccg
     7081 cgcgccggaa cgaggaggcg gccctcgaga agttcatcga ggccgatccc ggccgccgcg
     7141 ccgcctacgg aacggtgctg gccgacacgg cgcgcatcta cgacgggatg gcggcacgct
     7201 tccagtacga gatggtgctc gacaacctcg tgtcgatgtc cgcgctcctc cgtgcggcct
     7261 cgaacgtcgt cgcggcgggc gccggcgagc ccgctgcggc cccgcccggg cggggcgcct
     7321 cgaacccctt cgccacctac cacgagccgg ccgaggtgct cttcctccgg aagatcgcgg
     7381 ccatggccgg ggcgctgccc gaggggcagc ggatcgcgcc gatcgaccag cggctcgggg
     7441 gcgacttctc cgaggagaac gtcaggcgcc acgtcgaggc ggccgtggcc gccgcgacca
     7501 tcaagtcgca ggcggcgatg acagccgcca tgaaggaggg gcccgacgcc gtcgcccggt
     7561 caaccgatcc cttcatcgcc ctggcgcggg acctggcccc gctccagcag gaactgcgcg
     7621 acgtgcgcca gcgacgcacc gccgagctga acaggctgtc ggcgctgctc ctcgacgccc
     7681 ggcagcagta cctgaagaag gacttcgttc ccgacgcgaa cagcacgctc cgcttcacct
     7741 acgggcgcat ccggggctac tcgcccgccg acgcggtcgt ccacaagccc cacaccacgc
     7801 tggacggcgt gatcgagaag acgagagacc aggcgccgta cgacaccccc caggtggtcc
     7861 gcgagctggc gaaggcgcgc gacttcggcc gctaccggct gccgaagacc aaccagctgc
     7921 ccgtcggcct gctctacaac ctcgatacga cgggcggcaa ctcgggcagc gcgctgatga
     7981 atgccagggg cgagctggtc ggcgtgaact tcgaccgcgc gtgggccgcg accatcaacg
     8041 actatgcctg gaacgaggcg tacagccgct cgatcgccgt ggacatccgc tacgtgctgt
     8101 gggtgaccga gaaggtcggg aaggcggggt tcctgctgaa ggagatgggg atcggagact
     8161 gacgcgggag ttgttggttg ttagttgtta gttgttagtt gttagttatt agttattagc
     8221 tattagtcat tagttactgg ttggttatga gctgagtcgt tcggcttcgg gccacgctgt
     8281 cacgcgggtt cgaccgcgtg cagcgtggct gaagaccaag tgctcacttc gtcggcttgc
     8341 tgaacatcgc ctcgtcgatc gccttgttgt gctcgacctt gtcgagcgtg atcgacacgt
     8401 cctgcatgct gttggcgatc ttgatcgcga aggggatctt gaccccgtcc accgtccgga
     8461 agtcgtcggc cagcaccctc gactcgacct cgccgcccgc ttccggcacg tcctgcctgg
     8521 tcacctgctg gaggtcgagg aacgtctcgg cgtcgaagta gtacttggtg gggaagccgg
     8581 aggtgggcgt gaagagaatc accagggccg gccgcgtgcc gaccgtctcc ttgcccagca
     8641 gctcgatctt ggccccgcgt tccttgtagc cgagcagcgg ggtggggaag tactcgttca
     8701 gcatcgtctg gagctggctg ccggcgaggt cgcggtcgcc ctggaagctg ttgctcgccc
     8761 acccggtctt gccatcgcac cgctggtcca cgacgatctc gccggcgccc atggcgctga
     8821 ggtccatccg gaacgacatg cggctcttgt tcggcacggc cgagtagacc tcgaccgccc
     8881 cgctgagcgg cgtcccctgc accgtgacgg atatcgtgcc cgtcgcgacc cggttctgga
     8941 gcttctgaag cgccgcgcgg ccgccgatgg ccgccaggtg cttctcgacg acctcgtcgg
     9001 cggtctgggc ctgcgccgcc cccgcggcga accacgcacc cgccaacacg accagcagcg
     9061 acatcgcagc ccttctcatc tgattcctcc agtccggcgg acgcgtcccg tggcggccgc
     9121 ggcggtacag catagcgcag ggtcgccctc cgcgaccccg cgacgttgcc aacctctcga
     9181 acggcgcgat agagttgtgg gaacctgcct tgggaccatc cgcccccagg taagcccaag
     9241 tcaccccccg agcgaaaggg cgaagaaaag gcggcgggag aggtagaatg cccgcctgat
     9301 acctcgatgg tgaaggactg cctcattcat ccgatcagga accgttgaag cggagagcca
     9361 acatggacga gcagaacaag ggtcgtagcg tcatctcgcg ccgggggttg ctgggcgggt
     9421 ttgccgccgc cgccggggtc atgatcgtac cgcgccgcgt gctggggggg ccgggctacc
     9481 aggcgccgag cgacacgctg aacatcgcca ctgtcggcta cgcgcacggg atgggcacgg
     9541 ccaacaccaa caactgcggg aaagagaaca tcgtcgcgct gtgcgacgtg gacgagagcg
     9601 ccagggcggt cagggccctc gagcggtcga agatcctcga gaagttcccg aacgcgaagc
     9661 ggtacaagga tttccgggtg atgctcgacc agcagaagga catcgacgcg gtcatcgtcg
     9721 ccacgcccga ccactcgcac gcggtggtgg cgatggcggc gatgcagctg ggcaagcacg
     9781 tctacgtgca gaagccgctc acccggacga tctccgaggc gcgcgcgctc accgaggcgg
     9841 cgcggaagta caaggtcgtc acccagatgg gcaaccaggg gcactcgggc gagggcgttc
     9901 gcctgatcca ggagtggatc gaggacggcg ccatcgggaa ggtgcgcgag gtgcactgct
     9961 ggaccaaccg cccgatctgg ccgcagggga tgcccgcccc ggccgagggg cagcccgttc
    10021 ccgaggggct cgactgggac ctgttcctgg ggcccgcgcc cgagcggccg taccactcgg
    10081 cgtatcaccc gttctactgg cgctcgtgga tggactacgg ggccggcgcg ctgggcgaca
    10141 tggcctgcca cgtcatggac gccgcctaca ccgtcctgaa gcttggctac ccgaccagcg
    10201 tccagacgac cgtcgcctac atcgtgcagc cggtcacccg gcccgacgga tcccccggga
    10261 gccgccgcgt ggagtacaag gacagcttcc cgcccgccga gatcgtgcgc tacaccttcc
    10321 cggcccgctc gaaggacctc ccggaggtga agctgacctg gtacgacggc ggactgctgc
    10381 ccgagcgccc cgccgagctg gagcccgacc gccggctgcc ggagagcggc acgatcttca
    10441 tcggcgacaa gggcaagctg atgtcggaga cctactccga gggcccgcgc ctgattcccg
    10501 agtcgaggat gcaggcctac aagaggccca agaagacgct ggcgcgcgtc ccgggcacgc
    10561 acgaggaaaa ctggatcgac gccatcaagg gaaagacgcc gaagcccacc agcaacttcg
    10621 actactcggg tcccttcacc gaggcggtgc tcctcggcaa cctgggcgtg atgttccccg
    10681 ggcagaagct gctgtgggac gggccgaaca tgaaggttac caacgttccc gaggcgaacg
    10741 agtacgtcca gcatcactat cgccagggct ggagcctgta gcagggtact gaaggagggg
    10801 ctcgttcggc gccggcgggc gctgaacgag tcctgagctg aagggcaagg gagagggtag
    10861 aagggggaag acgcgggccg cgcggttcga gcggccccat gggagagaac atgcacaaga
    10921 ccaaccggcg gcggttcctc aagcaggcca ccgccgccac ggccggcttc atgattgttc
    10981 cccggcacgt gctggggggg acgggcttca tcgcgccaag cgaccagctg accaaggcca
    11041 tcatcggcgt gggcgggatg ggccgaaacc acattcccta cgagggcacc cgggtgcttg
    11101 ccgtctgcga cgtggaccgc acgcacctcg agaaggctct ctccatgtcg gggcccgacg
    11161 ccaagggcta ctccgactac cgtgaggtgc tgcagcggcc cgacatcgac gtcgtccacg
    11221 tggcgacgcc gccgcactgg cacgggctga tcgcgatcga ggccgcgcgc gccggcaagg
    11281 acatctggtg cgagaagccg atgacgcgga cgatcggcga gggcctgcgg gtgatcgacg
    11341 aggtcaacag gcacggccgg atgttccgcc tcaacacctg gttccgcttc aaggacacct
    11401 tctacggcct cggcaccgac gtgaagccgc tgaagaagct gatccagaac cgggtcttcg
    11461 ggtggccgct caccgtcacg gtcagcggcg tcaccggcta cgactggaag ttctactgga
    11521 gcggacgcac cgacctgacg ccgcagcagg ttccccccga gctggactac gagatgtggc
    11581 tggggccggc gccctacaag ccgtaccacc cgcaccgggt ccacgccaac ttccgcggct
    11641 actgggacta cgacggcggc ggcctcggcg acatggggca gcactacctc gacccggtgc
    11701 agtacatgct ggagaaggac gagacgagcc cgatccgcgt ggacgtggac gcgccgcagc
    11761 agcactacga cgcggccggg acgtggcgcc gcatcgagta cacctacgcg gacggctgca
    11821 agatcatcct cgacggcgag aaccgcgaca ccgaggcggc ctacatcagc ggcccgaagg
    11881 ggaagctgtt ccgcggcttc cgctccgaca ttcccaacat cgagaagatc gtcgcgtcgc
    11941 tgccggatcc cgagccgcag gtcggcacct tctccgactc ggtccgcacg cggcgcaagt
    12001 tcgcgctcaa cgagatgaac ggccaccgct cctgcacgct ggtgaacctg ggcatcctcg
    12061 cggtgcgcct cgggcgcacg ctgcacttcg atcccgaggc gcagcgcttc cgcgacgacg
    12121 aggccgcgaa ccggctgatc gatcagccga tgcgcgcgcc ctggatcatc tagtagcgca
    12181 gggctccagc cctgcggagt cccgcacggc ttcggccctc cgttcagcag agcgacgagg
    12241 agcagacgat gaagaacgca cgttggacat gcgcccgcac ggcgctggcc tccctggtgc
    12301 tgctcgcggt cctggcgccc gcgcccgcgt gggcgcagga caaccggacg ctggcgacca
    12361 aggtggccga catcctggcc ctgtttccgg ccggcagcgg cgaagaccgc gacaagctgg
    12421 ccgatcagat gattgccctg ggcgaacccg gcctggccga gttcacccgc cgacttgccc
    12481 ctgccggctc cggcgacgac accgccgtcc gctacgccgt caacgcgatg gcggtccacg
    12541 ccgcccgcgc cggcaacgag ccgaagcgcg ccgtcgccga acgcgccctg gtcaccgcgc
    12601 tcggctcggc aaccgacgtc gaggtgaaga ccttcctgct cagccagctc cgcgtggtcg
    12661 gccgcgaggc cgcggtcaag gccgcctcgc cgctgctcgc cgacgcggcg acggtcgagc
    12721 ccgccacgca gctgctgctg accgtgaagg gtcccctcgc gcgcacggcg ctggccgccg
    12781 cgctcgacaa ggcccagggc ccggcgcgga tcacgatcgt caaggcgatc ggcgagctgg
    12841 gcgcggtcga agcgaaccag aagctgctcg ccttcgccgc cgatcccgat cccgccatgc
    12901 gccgcccggc gctcgccgcc ctggcccgcc tggccagccc gcgatcgttc gacgccctca
    12961 ccggggcggc gacgaaggct ggcttcaagt acgagccggg caacgccatc ggctcgctgc
    13021 tgaactacgc ggcgcgcctt ggccagaccg gcaacgcctc gaccgctgaa aaggtgtgcc
    13081 ggctggtgat gcggaacacc gacgccccgg gcctgctcgg tacccgggcg gccgcgctcg
    13141 ccatcctcgc cgacgtccgc ggcccgcgcg cgctgcccga gctgatcgcg gcggtcgacc
    13201 atgccgacgt gagttacagg cacgcggcgc tggcccgcgc cgagaagatc aggagcgcgg
    13261 tcgcggtcaa ccagtgggtg gcgaaggccg agaaggtcga cccggtccgc cgcgccgaga
    13321 tcgtcgcgat gctcggccgg cagggcgacc gccgctcgct cccgttcatc cgcgcgtcgc
    13381 tggcctcggc cgatccggcg gtggcgctgg ccgccgccga ggggcttgcc ctgatggagg
    13441 ggccggcggc caacgaggcg ctggtcggcc tgctgaagag ccgctcggcc gccgacgccc
    13501 agcacgtggc cgacatcctg ctctggacga ccgacgccaa ggcgctcgac ccgctcgtgc
    13561 cgctgctgca gtcgggtgag ccggcccaga aggccgccgc ggtccgcgtg ctcggggccc
    13621 ggggcggaag ccgcttcgcg gcgcaggtgc tgccgctcgc ctcggacgca aaccccgagg
    13681 tgagggccgc cgcctacggc gcgctggccg gcgtggccgg gccgggcgac ctgccggcgc
    13741 tcctgaagct cgtcgacgcc gcgcccgatg cgcagctggc gcgcaacgcc cagcgggcgg
    13801 tcgtggcgtc ggccgagcag gtgacgcccg aggaggcccg tgccaggccg atcatcgcgg
    13861 cgatggacgg cgcggtgcat cccgaacgcc tcctcgaggt gctgccccag gtgggcggca
    13921 aggaggcgct cgacgcggtg gtctcgcgct tcgagggcgg gtcgggcgac acgaaggggg
    13981 cggccttccg ggcgctcgtg cagtggcgcg ggcccgaggc ggccgacagg ctcttcgcca
    14041 tcgtttcggc cggcgaaccc gcctaccgcg acatggcgtt cggcgggttc gtgcggcaga
    14101 tcggcatgtc gtcgctgccc gacgagcaga aggtgctgca gctgcggcgc gtgctgccgc
    14161 tggcgaagtc ggccaacgag cggcggatga tcatccggtc gctgcagcgc accaagacgt
    14221 tccaggcgtt cctcgtggcc tcctcgctga tggacgacgc cgaggtggcc aacgacgccg
    14281 ccggcgcggt gatgaccatc gcgctgccgg ccgcgggcga gaagaacggc ctcaccggaa
    14341 agctcgtgcg gaacgccctc gagcgggtga tccaggtgct gaccggcggc gagagcgact
    14401 acgacaagga gaacatcaag cggtacctgg ccgcgatgcc gcccgacgag gggttcgtgc
    14461 cgatcttcaa cggcacggac ctgacgggct ggcaggggct ggtggagaac ccgatcgtgc
    14521 gcgccaagat gtcgccgcag gacctggccg ccaggcagcc ggcggccgac gcgaagatgc
    14581 gcgagaactg gggcgtccgc agcggcatga tcgtcttcaa cggcagcggc gacaacctct
    14641 gcaccgtcaa ggagtacggc gacttcgaga tgctcgtcga ctggcgcatc accaaagacg
    14701 gcgacagcgg catctacctg cgcggcacgc cgcaggtcca gatctgggat ccggcccggg
    14761 tcgacgtcgg ggcgcaggtc ggttcgggcg gcctctacaa caaccagaag aacccgagca
    14821 agccgctcgt cttcgccgac aacccggtgg gcgagtggaa caccttccgg atcgtcatga
    14881 tcggcgagaa ggtgacggtc ttcctgaacg gcgtcaaggt ggtggacaac gtgaccatgg
    14941 agaactactg ggatcgctcg cagccgatct tcgcgcgcgg cccgatcgag ctccaggccc
    15001 acggcaccga cctgcagttc cgcgacatct acgtccgtga gatcggcgcc gaggcatcgc
    15061 aggggatcac gcccgaggag aaggccgagg ggttcgtcgc gctgttcgac ggaaccaacc
    15121 tcgatcaatg gatcgggaac aagaccggct acaaggtcga gaacggcatg ctggtcttcg
    15181 acccgaaggc tcccgacacg aagaacatct acaccgccaa ggagtacggc gacttcgtgt
    15241 tccgcttcga gttccagctg accccgggcg ccaacagcgg cgtcggcatc cgcacgccgc
    15301 ccgagggaga cgcggcctac gtggggatgg agatccagat cctcgacaac gacgccccgg
    15361 tgtacgccaa gctgcagccg taccagtatc acggctcggt ctacggcatc attcctgcca
    15421 agcgcggggc gttgaagccg gtcggggagt ggaacaccga ggagatcttc atccagggat
    15481 cgaagatccg gatcacggtc aacggtcagg tcatcgtgga cggcgacctg gtcgaggcca
    15541 cgaagaacgg gacgatggac cacaaggacc acccggggct gcagcggaag acggggcaca
    15601 tcggcttcct cagccacggg gcggtggtga agttccggaa catccggatc aaggacctga
    15661 cgaagtaggc tccgggctca aggctcaagg ctcagggtac gaaagaacgg cgagcacgga
    15721 acgggttccg tgctcgccgt tctcttttgg aactggtcct ctccgacgtc tccgcgcctc
    15781 tgcgcctctg cgtttgaacg tgggcaccct cagtacagct gatccgctcc gcgcccctgc
    15841 gcgatgagcg ggacgtaggt ttccagctcc ttctgcgtgg ccgggtcggt gagatcgatt
    15901 acccgcttcc gctcggccga gaggtaggcg gccatcacca gccgcgtcac ctcgagcccg
    15961 tatccccagt tgaggaagcc gtcgcgcccg gcgccgaagg cgtcgcgggc ctcggcgttc
    16021 tcgtcggtgt agccgtagag gtcggcctcg ttcacctgca cggcgagcag gccccgcgag
    16081 gcggtggact tctcgagcgc cagctccgcg tccttgaccg cctcggcggc cgcgtcgccg
    16141 atgaagatcg agagcggcga gaccagcgtg ttcacctcga acgcgtagcc cggccccgtc
    16201 ccgtccatga acagccgcag cccctgcttc tcgaacatcc acgagttggt gaactgcgcc
    16261 ttcacccgct gccccgtctc ggggttgcgg taggtgacga cgccggtggc gaagtcctcg
    16321 gccggcgcct tggcgtaatc gacgttccgg tcgcggagca gcttctcgcg ccactcccgc
    16381 tggccccact tgaggagcgc gacctcggcg ttgacctcga ccggctcgag gaaggtgacg
    16441 ggcttgccga tcggcgtgag cacgtgccgg ccgaccgcga tgctgtggca gcccatgtcg
    16501 ttcagcaccc cgccgccctg ccgcaccggg tcccagaacc aggcctcgtg ggggccgccg
    16561 tgctcctcgg acgagcgcga gagcgagagg ggccccatgc ggcgctgcac cggctcgagc
    16621 tgggccagct gcgccgtgat gggcttcatg aagatctggt tctcgaagta ggcggtccgc
    16681 aggttcaccg aggccgccag gtccaccagg cgccgcgcct cggccaccgt gcggcccagc
    16741 ggcttctcgc agatgacgcc tttcagggcg gcgcccgcct tcaccgcctc cacgatcgcc
    16801 tcgacgatcg agatgcgcgc gaagttgggc gcgaacacgg cgatcacgtc cacgtgccgg
    16861 gccatctcgg cgacgctgtt gtagacgacg gcgtcgccca ccatccactc gcgggcgagc
    16921 gaggccagct ccggcgcccc cgccgccgcc gagacgcccg tgacctcgat cccgcgcacc
    16981 tnnnnnnnnn ngacgagctg gggatccctg gtggtccttc ccaggcacct gcttcagggc
    17041 caggaggtgg aacttcgcca cgaacccggc cccgacgaac ccgtatcgca aagaggtgtt
    17101 catgatgatg tctcgagcgg gcggccgcga gggccgcccc ccggttcaat cccgaatccc
    17161 gattctcgat cgatgggtca gcgcgtaacc gccggcgttc cagcctgccg cgcgcccacc
    17221 ttcaggaaaa gagcaatcac gaacagcgcc gccgccatgc cggcgacgat ctgcaggctc
    17281 tgctgcacgg tggcgccggc gctcaggctg ccgacgatct gcggcacgaa ggtgccgccc
    17341 agcaggccaa ccgcgaagat gatgccgaag atgctgccgt acagccgcgg ctcgaacttc
    17401 gcgaacgtca ccccgacgat cgtggggaag atgggcgcga agaccaggcc ggtgaggacg
    17461 accgccacgc tggcgagcgt cgccgacgag gtcacgatca tcaccaggat cgtgcccact
    17521 gccaccagcg ccgcggcggc gatgagcgtc accccgatct tcgtcaggcc cttgatcgcc
    17581 gaggtgatga agcggccgat catcatggcg acgccgaaca ggctgagcac caggccggcg
    17641 ttccgggccg ccgacgggtt cgccgcgccg cccaggatct ccgtcatgta cggcttgctc
    17701 caggtcgtca tgctcgactc gagcgcgatg tagcagaaca gcgccagggc cgccacgatc
    17761 accgccggat gacgcagcag cgcgaacgcc tccgagaact ggtatcccgt cgagaccatc
    17821 gggaaccggg tccccatcgc catcacgaag ccgatgagga agagcgccgc cagcaggacc
    17881 agcccggccc ggtagttcac cagcgagaag accagcggcg tcaccaccag ccccaggccg
    17941 aagaacccgt tgccgaagtt gctcgcccgc gccgggtcct tgcccccgaa caggacgacc
    18001 gggatcagcg tattgcccac ggtgttgagg cacatcgcgc ccacccccgt caggatgcac
    18061 gcgaggatgg ccatctcgta gctccccgcc acgcgtagca ggatcatgct caacgccgcc
    18121 accgcgaagc cgaccagcgc gaccggcttg tagccgaagc ggtcgaccga cggcccgatg
    18181 acgagctgca cgatggcgct cgtgaggaag agcgcgaagg tcaggttccc gatgttctcg
    18241 atgttcagcg ccgcctggta ttcgaccgag atggccccca cgatgacgaa gcagatcgcc
    18301 aggccgaaga ccgccatcag ggcgacgatc gacgtgttcc gcgtttcgct cgcttgcatg
    18361 tcccactcct tcgggggcgt ctgccctgaa tcccgactcc tgattcccga atcctgaatc
    18421 ctacgcgatc accctcttca gcccggcgaa cgactccttc acgcactcgg ccggcgtctt
    18481 gccgtcgggg tacagctcct gctcgagcac gatccactcg gtgccgccgt ccgaccggca
    18541 cgcatcgtac acctgccgcc acggcacgga gtcgagcgcc aggatcggct tcttccccgc
    18601 gtccccctcg cggacggtcg gcttgaagtg cacggtccgc gaccgcccgg ggtatttctt
    18661 cacgtaggcc accgggtcgt gcccggcggc gaaggtccag ccgcagtcct gctgcaggat
    18721 gacctccttc ttcgtccgct ccgcgaagag gtcccagaac gtcttgccgc cgtcggtctt
    18781 gaactcggcc gtgtggttgt ggtacccgca cgccatcccc agcggcgcca gctgctcggc
    18841 cgcccggttg aacgtctccg cgagcgcctt gctcttctcg gggtcggtga agtcgcggct
    18901 gctgggaacc accaggaagc ggcacccgat cgcctggtgg aactcgatgg cctgcttcag
    18961 cgcgtcgccc tgcagctggt cggcgccgat gtgcgtaccg gcggccttca ggccgaggcc
    19021 gtcgagccgc ttgcggagtt cggccccccg gccggcgtac ccgaagtagc cggcgaactc
    19081 cacgcccgtg aatcccatcc tggacaccgc gtcgaggcag gcgtcgaagt ccgcctggca
    19141 gtcgccgcgc agcgagtaga gctgcagggc aatcgggatc gcggagtcgg cccggacccg
    19201 tgccgcggcg gccatcgcca gcggcgccgc ggctgtcacc tccagaaacg accggcgcga
    19261 cagcgcgccc cctccacgcg ttcggttcgt catcgcttcg gctcctgtcg gcgcccgccg
    19321 gcctcgcgag cctgctccgt tagacgcgtt gccgcgggct gctgtagaat cgcgcgcatt
    19381 ataggtcact ggaaggacgc catggtcaag aagcgtgcgt gcagtcggcg ggatttcctc
    19441 aggaccgccc ctttggccgt cgcgggggcc gccctcgctc ccaccatcgt caaggcctcg
    19501 gcgctcgggc gcgggggagc ggtggcgccg agcgaccgga tcgtgatggc cgggatcggc
    19561 ttcgggatgc aggggccggg gaacatgcgc tcgttcctgg ggaaggacga ggtgcagtgg
    19621 gtggcggtct gcgacctgga cgatgaccat ctccaggcgg cgaggcggac cgtcaatgag
    19681 aagtacggca acgaggattg cgccacctac aaggactacc gcgagctgta cgcccgcggc
    19741 gacctcgatg ccgtttcgat cgcggtgccc gatcactggc acgcgatcct ctcgatcgag
    19801 gcggtgcgcg cgggcctcga cgtgttcggc gagaagccct tcacgcacag cctgcgcgag
    19861 ggccgcgccc tctgcgacgc catccgccgc tacggccgcg tctggcagac cggcagctgg
    19921 cagcggtccg aggagaactt ccaccgcgcc gccgagctgg tgcgcaacgg ccgcatcggc
    19981 aaggtgcttc gcgtggaagt cggactgccc tccggctaca ccgatttcgc ccgcaccttc
    20041 ggccaggaga cgatcgagcc gccgcccgcc gccctcgact acgaccggtg gctggggccg
    20101 gcgccctacg cgccctactg caaggctcgc gtgcacatga actggcgctg gaacatggac
    20161 tacgccggcg gccagctgat ggactgggtg gggcaccacc tcgacatcgc ccactg
//
LOCUS       DAOPQX010000023        20178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3292_length_20178_cov_5.016946, whole genome shotgun sequence.
ACCESSION   DAOPQX010000023 DAOPQX010000000
VERSION     DAOPQX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 20178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20178
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3292_length_20178_cov_5.016946"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PKK95_02395"
     CDS             complement(<1..1347)
                     /locus_tag="PKK95_02395"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17084.1"
                     /translation="MPSEMNRREFLAGAAAAAATFTIVPRHVLGGTRYVAPSDRITLG
                     CVGFGTQAIREIGGILASPDVEVVAVCDVEKDGVNYLEWGKNQLRDGIRRLLEEPAWR
                     EGFNHVPGGRDVGKEIVDTYYRKFREGGVSKGCATYTDFREMLEKEKDLTAVKVMTPD
                     HTHAAISIAALQKGLNVIVHKPLANRVLEARAVIEAARAAKRATHFLPASEGTNQRLA
                     LEMVKNGAIGTLREIHNWSMRPMWPQFAKLPADTPPVPAGFDWDLWLGPSLDRPYHPA
                     YTHTNFRGWYEFGGGSIADMGHYSLWPIFQALELDPPVSVESTPSHVCDAADEVCFRI
                     RNDYSFPAACTIRMRFAAKGSRPALDIFWYDGGIKPPVPEELMAENKELAEEGMLFVG
                     DTGKILGGFRAENPQLIPEAKMKAYRAANNIPEPAPPQPRERRERGERPGPSERDVA"
     gene            complement(1512..2777)
                     /gene="hisD"
                     /locus_tag="PKK95_02400"
     CDS             complement(1512..2777)
                     /gene="hisD"
                     /locus_tag="PKK95_02400"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOC17085.1"
                     /translation="MTRLRRIAIEEVPEARVALPGEATTREAGRIVEDVRARGEEALR
                     EHAERLGDLGPHDRLTWDRQSLDRARDAVEPAARAVLERAAESIGHFAAAQRESLREV
                     RVAIPGGWAGHSMAPVASAGCYAPGGRFPLPSSVLMTVVTARAAGVSSVWVASPRPSP
                     VTLAAASIAGADGLLAAGGAHAIAALAFGAGPVPRCDLIAGPGNRWVTAAKRIVAGEV
                     GVDLVAGPSELVVLADLSADAELIAADLLAQAEHDPDAVPILVTPDPQLAARVEAVVE
                     ARLPRLATAATAAAALANGGIVLVPTIEAGIEVCNRLAPEHLEVITREPNAIAARLVH
                     YGALFLGRGAGEVLGDYGAGPNHVLPTGGAARFSGGLSVFTFLRVRTWMQIDDLDAAR
                     PLLRDTAHLARLEGLDGHAQAAEARLAGR"
     gene            complement(2774..4045)
                     /gene="hisE"
                     /locus_tag="PKK95_02405"
     CDS             complement(2774..4045)
                     /gene="hisE"
                     /locus_tag="PKK95_02405"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOC17086.1"
                     /translation="MPSIDLMDGRAVQLVGGRERALDAGDPRPLARRFGRVGEVAVID
                     LDAALGRGSNASLVAELCRLAPCRVGGGIRSVEDARRWLDAGAVRVILGTAATPGIIS
                     ALPRDRVIAAVDAIGGEVVVEGWRTATRERAEEKVRTLRGLVWGFLGTFVEREGRMEG
                     TDLDRARALKAAAGDACLTAAGGITTTEDVAALDRDGIDAQVGMALYTGRLPLADAFA
                     APLRSDRPDGLWPTVVADEAGRALGLAYSSLESLRAGLESGAGVYHSRRRGVWTKGEA
                     SGNRQDLLRVEADCDRDALRFTVRQHGEGFCHLGTATCWGRPRGLAALEARLRERLTV
                     PDTDSYTRRLLTDPDLLAAKLVEEAGELSEARDAANAACEAADLVYFTAVALARAGVA
                     FADVERELDRRALRVTRRPGDAKPPAEGRGR"
     gene            complement(4048..4800)
                     /gene="hisF"
                     /locus_tag="PKK95_02410"
     CDS             complement(4048..4800)
                     /gene="hisF"
                     /locus_tag="PKK95_02410"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOC17087.1"
                     /translation="MLTVRVIPCLDCAGGRVVKGIKFENLRDAGDPVERATVYERQGA
                     DELVLLDVSATPEDRGHRLETVRAVREGLGIPLTVGGGVRSPRDAASLLEAGADKVAV
                     NTAAVRQPELVASIAARFGSQCAVVAIDAARKGGGWEVVVESGSSRTGCSAIEWAREA
                     CARGAGEILLTSVDRDGTRGGYDLDLIAAVSAAVSVPVIASGGASSSSHLVEAVRAGA
                     DAVLAASIFHDGERSVAEVKAFMAASGVEVRL"
     gene            complement(4794..5402)
                     /gene="hisH"
                     /locus_tag="PKK95_02415"
     CDS             complement(4794..5402)
                     /gene="hisH"
                     /locus_tag="PKK95_02415"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOC17088.1"
                     /translation="MTRDVVVVGTSVANIASVLAALRRAGTRPVVSLDPVEVDRAEHV
                     VLPGVGAFGAGMEAIARAGLTEALAGRVAAGRPLLAVCLGLQLLAEGSEESPGVRGLG
                     VLPGIARRYGAAVRVPQLGWNRVWPVEGARVIEPGSAYFANSYRMESIPPGWSGATAE
                     YGGPFVAALERGAVVACQFHPELSGAWGAALIRRWLEMEGAC"
     gene            complement(5399..5983)
                     /gene="hisB"
                     /locus_tag="PKK95_02420"
     CDS             complement(5399..5983)
                     /gene="hisB"
                     /locus_tag="PKK95_02420"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOC17089.1"
                     /translation="MSRKASVSRETRETKIELALRLDGEGRSAVKTGVGFLDHLLATL
                     ALHARFDLDLSCEGDLQVDDHHTAEDCALALGEALDRALGTRAGIARFGWAFAPLDEA
                     LARAVVDLSGRPFARVRLGLERDRLGGLACENVRHVLGSLASTARLTLHVDVLEGDND
                     HHRAEAAFKALALALRQAVALDGSAGVPSTKGSL"
     gene            complement(5980..7701)
                     /locus_tag="PKK95_02425"
     CDS             complement(5980..7701)
                     /locus_tag="PKK95_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC17090.1"
                     /translation="MTRRPTARLRAITPYAVPRLPTPIDLPLDGNEGPAPEAERLAEE
                     AGPLAAAISRYPDPSALQQLWADRLGVPPERVRITAGADEALDRIIRAFVGPGREMLT
                     TAPTFEMFERYCALAGGRYVDVPWTGGPLPLEALLSRVTRRTGVIAIATPNNPTGTCA
                     STGALLELSARAPDVVVLVDLAYVEFADEDPTSALLERPNVMLTRTVSKARGLAGLRV
                     GCAAGPADLVGWLRAAGNPYSVSGLSLAVAAASFRSDERRVNEAVAEVRREREALARL
                     LAPLASRVWPSQANFVLAEFPKVRWTWEALAGLGIGVRCFPGRPYLENALRIACPGNA
                     ADFARLERGLRAVLEPQALLFDMDGVVADVSASYDEAIVQTAAAFGAAIDRGEIAAAR
                     ARGGANNDWEVTRRLMAAHGVEQPLEAVTARFEQIYQGSKESPGAPGLCERERLIPAP
                     PLLERLAARLPLGIVTGRPRRDAIRFLDRCGLRDRFSVLVTMEDGPLKPDAAPVRLAL
                     ERLGVGRAWMVGDTPDDMRAARSAGVVPIGIVAPGHAPETMRGALFEAGAGRVLGDLA
                     ELEELLP"
     gene            complement(7711..8586)
                     /gene="hisG"
                     /locus_tag="PKK95_02430"
     CDS             complement(7711..8586)
                     /gene="hisG"
                     /locus_tag="PKK95_02430"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOC17091.1"
                     /translation="MTPIVDPNVLRLALPKGRMQDEVFSLLAAAGLRVKAGTRGYRPT
                     ISLPSCEVKLLKPQNIVEMLHAGSRDVGFAGADWVAELGAEVVEVLDTGLDPVRVVAA
                     APEALVQDGRLPDTPLVVASEYERLSRGWIARRGLQARFLRSYGATEVFPPEDADLVV
                     DNTATGATLEANGLVVVDELMPSTTRLFAHPRSLEARGRREAIERFALLLTSVLDARR
                     RVMLEVNVSAERLEAVCQILPCMREPTIAPLHGGAGYALKAAVLRDELAAVIPRIKAA
                     GGSDIVVTSPSNIVA"
     gene            9043..>9330
                     /locus_tag="PKK95_02435"
     CDS             9043..>9330
                     /locus_tag="PKK95_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17092.1"
                     /translation="MFLITDLRRAAASFMRTPGFAAVAVLALALGIGVNTAIFTVVSG
                     VLLRPLPYPEPERLVAIYERVPDNPRLAVAPPSIADWAGNRAFSSWAVTAGD"
     assembly_gap    9331..9340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9341..11451
                     /locus_tag="PKK95_02440"
     CDS             <9341..11451
                     /locus_tag="PKK95_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17093.1"
                     /translation="KFRFKRADAMKRLEATAVSGDFFQTLQVAPERGRWLEPRDDQAG
                     AEPVVVLGHALWKRSFGGDPSIVGRQVTLTDKSYTVAGVMPPGFAFPGETEAWVALQL
                     PADQLLDDNRYSHYLAGVGRLAPGVGIEKASASLNAIAARVGARFGDGYAGRGVNLVP
                     LRADLVGDVRPALLLLLGAVGFVLIIACVNVANLLLARSAERDREVAIRAALGASQLR
                     LASHFAAEGLVLTIAGGLAGLLLAAWARDAILWLVPADLPRLDEVSLDWRVFAFAAST
                     AIVTGLLLGLAPAVFARRRAHASRLASRGTAGSRERARVQSALMIAEVALSLILLLGA
                     GLLANTFWRLTAVDPGFKAADVLTFEVMLPPPAYPAAEARARFQEDVLARLARLPGAR
                     RAGAATNLPLSGTNMAFAFFYPGESMETAKPHPANYRAVSPGYLEAIGVPLVAGRWLA
                     ESDRADRAPVVVVNQAFARRYFPGRQAIGERISITRGRDLVWREIVGIVGDLRHRALS
                     ADADPEMYVPFQDDPVFFMRFALNAANAESLAGSARAAVWEVDRNLPVTKMRPLEALV
                     AASVAQPRFHALLVGGFALLALVLAAIGLYGVVARLVAQRTQEIGVRSALGASPRQIL
                     ALVGDHVSRLVLAGLLIGLAGALALTRTLERFLFGVQPTDVSTFAAVTAIFALVAGVA
                     AWLPARRALRVDPVEALRAE"
     gene            complement(11462..11893)
                     /locus_tag="PKK95_02445"
     CDS             complement(11462..11893)
                     /locus_tag="PKK95_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOC17094.1"
                     /translation="MYRQILVAIEHSVADETIVEHVEKLARLAGSRLLLVHVADGWAA
                     RHFDELNLRESEEMREDRAYLEALEGQLRGRGIEVESFLAMGDPASEICRVAHDRAVD
                     LIAMATHGHRGVSDIIHGQTADAVRHRVSIPVLLLRAKRPR"
     gene            complement(11897..13204)
                     /locus_tag="PKK95_02450"
     CDS             complement(11897..13204)
                     /locus_tag="PKK95_02450"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOC17095.1"
                     /translation="MDGRPASLPEVHGTVGVPQGAGFWRKLFAFTGPGYLVAVGYMDP
                     GNWATDLAGGARYGYRLLSVIFISNLLAILLQALAARLGIASGRDLAQACRDRYPRWA
                     AVSLWVLAEVAIAATDLAEVIGSAIALHLLFGLPMALGVCLTSLDVLVVLYLQHHRFR
                     YVEALVVLLIAGIGGAFALELYFSKPDPLQVLGGFMPAPELLRDREMLYLAVGILGAT
                     VMPHNLYLHSSIVQTRKYGDSEASRAEAASFATLDSTVALTSALFINGAILIVAAATF
                     HGTGYEDVADLSDAYKLLAPLLGTSLAATLFAVALLFAGQNATLTGTLAGQIVMEGFL
                     NLRLRPWLRRFVTRLIAIVPALATVIAYGDRGAGELLILSQVILSLQLPFAIFPLVAF
                     AGDRRIMGKMAIGRGLKLLAWPAAVVIAGLNVWLLAQVVAGLV"
     gene            complement(13222..13770)
                     /locus_tag="PKK95_02455"
     CDS             complement(13222..13770)
                     /locus_tag="PKK95_02455"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HOC17096.1"
                     /translation="MRRLVHTAATAAIALAFAAAAGAQQTTQVHPGKGGSPHVKSQWT
                     VDGATIAITYGRPSLKGRTVGKEVAPFGKEWRTGADEATTLETDKALTFGTLQVPAGT
                     YTLYTVPGESDWQLIVSKKTGQWGVPYPSGEDLGRVPMMVEKLDSPVEQLTISIDDTP
                     TGGTLAVSWGTTKASTAFTVAK"
     gene            complement(13821..14849)
                     /locus_tag="PKK95_02460"
     CDS             complement(13821..14849)
                     /locus_tag="PKK95_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17097.1"
                     /translation="MRPSTRTASACAALLAAAAGVALAAGQPAQPAASPVDVAFQEWP
                     TPTSQSFPHDPAVAPDGSAWYTGMRANLLGRLDPATGKIREYKLPTENTGPHGIVADR
                     EGNIWYTGNYSGLIGKLDPKTGKVTEYKMPDPKARDPHTLIFDQAGTLWFTVQGSNFL
                     GRLDPRTGKVDLVPAPTPDSRPYGIVVDSRGVPYFDLFNTNKIGRIDPSTLAIREYEL
                     PSPGARPRRIAIGKDDTVYYTDYARGMLGRLDPATGKVTEFPTPGGSGSRPYGMAVTS
                     DGAVWFSESGVEPNTLVRFDPATGKMQAWPIPSGGGVVRHMVAAPNDELWLACSGVDR
                     IARARIGS"
     gene            14950..15540
                     /locus_tag="PKK95_02465"
     CDS             14950..15540
                     /locus_tag="PKK95_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17098.1"
                     /translation="MRPLLALAGLLVALPVVAAYQNGPMPGVTGGFGEPTCTMCHQGP
                     PPGPKDGRLSLEAPREFRPGQTYQLRVTLTRRNLAVGGFEVAARFQTRPAAGAQAGTL
                     RSLDPRTQVVTGAKGVQFAQHTQAGSKAVPPGKMTWVVEWRAPEQAPGNVLFHVAANA
                     ASGDASPLGDLIHTLEVVSRPAPAARAGSPAPVSAH"
     gene            complement(15799..17355)
                     /locus_tag="PKK95_02470"
     CDS             complement(15799..17355)
                     /locus_tag="PKK95_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC17099.1"
                     /translation="MRLHEMFVRSAKKHAKKLAVADRATGQRVTYKDVLLRALILAAK
                     LDGREAGGFVGVMVPTSAGCMYATLAVLLSGRTPVMINYSTGAADNCRDAQRRLAFST
                     ILTSKALLARINCPLIDGMVCLEDLAAGVSLLDKLKGAARAARSADRIIRKIPKGDAE
                     DYAVVLFTSGSEREPKAVPLMHANIAANLEGVAQVVDFSAEDVMMANLPFFHVFGLTV
                     NMWLPLWQGMTIVTYPNPLDFRSVCQAVRDEKATVMVGTPAFLNGYLQKSEPGDFATI
                     RLLITGADKCTEALRRGWWEKHRIILLEGYGTTETSPVISVNAPQHNRPGSVGRPLPN
                     VEVRLEHYETGEECAVNQIGKVLVKGPSVMNGYFDDFEATSLHLRHGWYDTGDMGYMD
                     DDRYLWHVGRLRRFLKVGGEMVSLVRVEDVLDRLLPDDCQCCVVEVPDAARGARIVAA
                     VTASVDEKEILAQMAESLPRIALPKQFVVLPELPKMSSGKLDFRTITEMVRDAVQKRP
                     PVGPASVPAE"
     gene            17451..>20178
                     /locus_tag="PKK95_02475"
     CDS             17451..>20178
                     /locus_tag="PKK95_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOC17100.1"
                     /translation="MPSRPGRLFLILAFVAGASPWWPAADAAAQGAAPPQAAPSQAGP
                     AAPGRPITADDYARGERFLPQNVEPLVIGGRVTANWLPGDRFWYRNDLADGHEFVLVD
                     PAARRRARAFDHARLAAALSAASGRAYAPNALPFQWIGISADGRQITFDLEGRRWSCD
                     ALGSRCADTGASPEPAPVGAAPARRGPRTKAASLPSGDGNLRVRELATGRERQLTTDG
                     APYFGYATDNAGWRSSDRPIVLWSPDSRRIATYQQDEREVGEMYLVNTPIDPVTHPRL
                     RVSKFPLPGDRHVAMLHRVVIDVETGRVTRLAMPPDYHRATLGDDVSLDDWQWKPDGS
                     AFAFISTSRDHKEAVLRVADTATGQVRTVMSEKVATHYESRVGCRVLWDSNEVIWYSE
                     RDDWGHLYLYDLATGALIRRITAGEWPVMALLRVDEKARTLWFEAQGREKGEDPYFRH
                     VYRIGLDGTGLTPLTPVEGDHSVQLSPSGRYLVDTYSRPGVEPVVALREGEGRFVMML
                     EKADITRLRAAGWRPPIPITVKARDGKTDLHGLMFRPTRFDPARKYPVINYVYPGPQT
                     GSTGSRSFSAARGDRQALAELGFIVVTIDGMGTPGRSKSFQDAYYGAMGRDNTIPDQI
                     AGMKELAARHPWFDVSRAGIWGHSGGGFATTTAMFRFPGFFKAGIAESGNHDQRLNED
                     DWGERYQGLLVRDGQGGDNYAAEANQLYAQNLEGRLMLAHGTMDTNVPPYQTLMVADA
                     LIKANKDFDLVLLPNQDHGYGDASAYMMRRRWDFFVKWLMGTEPPKEYRFGRQGPGPA
                     RRAPGELRFGPARREPTRADVLRGNWDRYRSNNDLLFYHLDVRVDPERKWIGGKNTIR
                     FRMTRDDTRIQIDLAKALAVDRIVFGGRELRYEREEGAVFIDFPETLRRGREY"
BASE COUNT         2858 a         7350 c         7067 g         2893 t
ORIGIN      
        1 ggcgacgtcg cgctcggacg gcccgggccg ctcgccgcgc tcccgcctct cgcggggctg
       61 cggcggcgcc ggctcgggga tgttgttcgc cgcccggtac gccttcatct tcgcctcggg
      121 gatgagctgc gggttctcgg cccggaatcc gccgaggatc ttgcccgtgt cgcccacaaa
      181 cagcatccct tcctcggcca gctccttgtt ctcggccatc aactcctcgg gcaccggcgg
      241 cttgatcccg ccgtcgtacc agaagatgtc gagcgccggc cggctgccct tcgcggcgaa
      301 ccgcatccgg atggtgcagg cggccgggaa cgagtagtcg ttccggatgc ggaagcagac
      361 ctcgtctgca gcgtcgcaca cgtggctggg cgtcgactcg acgctgacgg gcgggtcgag
      421 ctcgagggcc tggaagatcg gccagaggct gtagtgcccc atgtcggcaa tcgatccgcc
      481 gccgaactcg taccagccac ggaagttcgt gtgggtgtag gccgggtggt acgggcggtc
      541 gagcgacggg cccagccaca ggtcccagtc gaacccggcg gggacgggcg gcgtgtcggc
      601 cggcagcttc gcgaactgcg gccacatcgg gcgcatcgac cagttgtgaa tctcgcgcag
      661 ggtaccgatg gcgccgttct tcaccatctc cagcgcaagc ctctggttgg tgccctcgct
      721 ggccggcagg aagtgggtgg cgcgcttggc cgcacgcgcc gcctcgatga cggcgcgggc
      781 ctcgagtacc cggttcgcca gcggcttgtg gacgatgacg ttgagcccct tctgcagcgc
      841 ggcgatcgag atggcggcgt gggtgtggtc gggcgtcatc accttcacgg ccgtcaggtc
      901 cttctccttc tcgagcatct cgcggaagtc ggtgtaggtg gcgcacccct tcgacacccc
      961 gccctcgcgg aacttgcggt agtaggtgtc gacgatctcc ttgccgacgt cgcggccgcc
     1021 cggcacgtga ttgaacccct cgcgccaggc cggctcctcg agcagccgcc ggattccgtc
     1081 gcgcagctgg ttcttccccc actcgaggta gttgacgccg tccttctcga cgtcgcagac
     1141 ggcgaccacc tcgacgtcgg ggctggcgag gatgccgccg atctcgcgga tggcctgggt
     1201 gccgaagccc acgcagccga gcgtgatccg gtcgctcggc gcgacgtagc gcgttccccc
     1261 gagaacgtgc cgcggcacga tggtgaaggt ggcggcggca gctgccgccc cggcgaggaa
     1321 ctcgcgtcga ttcatctcgg atggcatctt ctcgcctccc cgtgtttcgg taggtcgggc
     1381 gtgacaacgg ctccggcgca cttccccggc ttcttcgcgc gccgatcata caacctcgcg
     1441 gcggctgcgg cgacgcgaag ccgctggcgc gcggcggttt gtcgaatgac gcagcctgaa
     1501 ggcgagaggg atcagcggcc tgccaggcgc gcctcggcgg cctgggcgtg cccgtcgagc
     1561 ccctccaggc gggccaggtg cgccgtgtcg cgaaggagcg gcctggcggc gtcgaggtcg
     1621 tcgatctgca tccaggtgcg gacgcggagg aacgtgaaga ccgacaaccc gccgctgaag
     1681 cgcgccgcgc cgccggtcgg cagcacgtgg ttggggcctg ccccgtagtc gcccaatacc
     1741 tcgcctgcgc ccctgccgag gaacagcgcg ccgtagtgca cgagccgcgc cgcaatcgcg
     1801 tttggctcgc gcgtgatgac ctccaggtgc tcaggggcca ggcggttgca cacctcgatt
     1861 cccgcctcga tcgtcggaac caggacgatt ccgccgttcg cgagcgcggc cgcggcggtg
     1921 gcggccgtcg cgagacgcgg cagacgcgcc tccaccaccg cctcgacccg ggccgccaac
     1981 tgggggtcgg gagtcacgag gattggcacc gcgtcgggat cgtgctcggc ctgcgcgagc
     2041 aggtcggcgg caatcagctc ggcgtcggcg ctcaggtcgg ccagcaccac gagctccgac
     2101 ggcccggcca ccaggtccac gcccacttcg ccggcgacga tccgcttggc cgccgtcacc
     2161 cagcggttgc cgggcccggc aatcaggtcg caccgaggaa cgggccccgc gccgaacgcg
     2221 agcgcggcaa tcgcgtgggc gccgccggcg gcgagcaacc cgtcggcgcc tgcaatcgag
     2281 gcggctgcca gcgtcaccgg cgagggccgc ggcgatgcca cccacaccga agacacgccg
     2341 gcggcccgtg cggtcaccac cgtcatcagc acggacgagg gcagtggaaa ccggccgccc
     2401 ggggcgtaac agccggcgct cgcgacgggc gccatcgagt gccccgccca cccgccgggg
     2461 atcgccaccc gcacctcgcg caggctttcg cgctgtgcgg ccgcgaagtg ccctatcgat
     2521 tccgcggcac gctcgagcac ggcgcgagcg gccggctcga ccgcgtcgcg cgcgcggtcg
     2581 agcgactgcc ggtcccacgt cagccggtcg tgtggcccga ggtcgccgag ccgctctgcg
     2641 tgctcgcgca gcgcctcctc gccgcgggcg cgaacgtcct cgacgatccg tcccgcctcg
     2701 cgggtggtgg cctcgcccgg aagcgccacg cgtgcctccg gcacctcctc gatcgcgatt
     2761 ctcctcagcc gcgtcaccgc cccctccctt ccgccggcgg cttggcgtcc ccaggccggc
     2821 gcgtgaccct cagggcgcgg cgatcgagct cgcgttcgac gtcggcgaat gccacccccg
     2881 cacgggcgag cgccaccgcg gtgaagtaca ccaggtcggc tgcctcacac gccgcgttgg
     2941 ccgcgtcgcg cgcctcgctc agctcccccg cttcttccac gagcttcgcc gcaagcagat
     3001 cggggtcggt cagcagcctt ctcgtgtagg agtctgtgtc gggaaccgtc agccgttcgc
     3061 gcaggcgggc ctcgagggcg gccagccccc gcggtcgccc ccagcaggtt gccgtaccca
     3121 ggtggcagaa accctcgccg tgctgccgca ccgtgaagcg caaggcgtcg cggtcgcagt
     3181 cggcctccac gcgcagcagg tcctgccggt tgccgctggc ctcgcccttg gtccagacgc
     3241 cgcggcgccg cgagtggtag acgcccgcgc cgctctcgag gccggcgcgc aggctctcga
     3301 ggctcgagta cgccaggccg agtgcgcggc cagcctcgtc ggcgaccacc gtcggccaca
     3361 ggccgtcggg gcgatcggac cgcagcggcg cggcgaaggc gtccgccaac gggaggcgac
     3421 ccgtgtacag cgccatgcca acctgggcgt cgatgccgtc acggtcgagc gccgcgacat
     3481 cctccgtcgt ggtaatgccg ccggccgccg tgaggcacgc gtccccggcg gcagccttga
     3541 gcgctcgggc gcgatcgagg tcggtgcctt ccatccgccc ctcgcgctcg acgaaggttc
     3601 cgaggaagcc ccacaccagg ccgcggagcg ttctcacctt ttcctcggcg cgctcgcgcg
     3661 tcgcggtgcg ccacccctcg acgaccacct ctccgccgat tgcgtccacc gccgcgatca
     3721 cgcggtcgcg gggcagcgcc gagatgatgc ccggcgtcgc ggccgtaccg agaatcaccc
     3781 ggacggcgcc cgcgtccagc cacctccgcg cgtcctcgac gctgcgaatc ccgccgccga
     3841 cccggcacgg ggcgagcctg cacagctcgg cgaccagaga cgcgttgctg ccgcggccga
     3901 gcgccgcatc gaggtcgatg acggctacct cgccgacgcg gccgaaccgg cgcgcgagcg
     3961 ggcgggggtc gcccgcgtcg agcgcacgct cccggccgcc gaccaactgc acggcgcgcc
     4021 cgtccatcag gtctatcgag ggcacgatca cagccgcacc tccacgccgc tcgccgccat
     4081 gaacgccttc acctcggcca ccgagcgctc cccgtcgtgg aagatcgacg ccgccagcac
     4141 cgcgtcggcc ccggcgcgca ccgcctcgac gaggtgcgac gagctgctcg ccccgcccga
     4201 cgcgatcacc ggcacgctga ccgcggccga gacggcggcg atgaggtcga ggtcgtagcc
     4261 gccgcgcgtg ccgtccctgt ccacactcgt caggaggatc tcgccggcac cgcgcgcgca
     4321 ggcttcccgg gcccattcga tggccgaaca cccggtgcgc gaggatcccg actccaccac
     4381 gacctcccag ccgccgccct ttcgtgccgc gtcgatggcg acgaccgcgc actgcgagcc
     4441 gaacctcgcg gcaatcgagg cgacgagttc cggctggcgc acggccgccg tgttcacggc
     4501 caccttgtcg gcacccgcct cgagcaggct tgccgcatcg cggggcgacc ggacgccgcc
     4561 gcccaccgtg agcgggatgc cgagcccctc gcgcacggca cgcacggtct cgaggcggtg
     4621 gcctcgatcc tcgggcgtgg ccgacacgtc gagcagcacc agctcgtcgg ccccctgcct
     4681 ctcgtagacc gtcgcccgct ccacagggtc gcccgcgtcg cgcaggttct cgaacttgat
     4741 ccccttgacc acgcggccgc ccgcgcagtc gaggcacggg atcacgcgca ccgtcagcat
     4801 gccccctcca tctccagcca ccgccggatc agcgccgcgc cccacgcgcc cgacagctcg
     4861 gggtgaaact ggcacgcgac caccgccccg cgctcgagag ccgccacaaa cggccctccg
     4921 tactcggcgg tggcgcccga ccagcccggc gggatggact ccatccggta cgagttggcg
     4981 aagtaggccg agcccggctc gatcacgcgg gcgccttcca ccggccagac ccggttccat
     5041 cccagctgcg gtacgcggac cgccgccccg tagcggcggg caatgccggg caggacgccg
     5101 agaccgcgca cgcccgggct ctcttcgcta ccctcggcca gcagctgaag gccaagacag
     5161 acggcaagca gcgggcgccc cgcggccacg cgccctgcga gcgcctcggt gagtccagcg
     5221 cgggcgattg cctccatccc ggcaccgaag gcccctactc cgggcaggac cacgtgctcc
     5281 gcgcgatcga cctccaccgg gtcgagcgac acgactggcc gtgtgcccgc ccggcgcagc
     5341 gcggcgagca ccgaggcgat gttggcaacc gacgtcccaa ccaccaccac gtcgcgtgtc
     5401 acaggctccc cttcgtgctc ggcacgcccg cgctgccgtc gagcgccaca gcctgccgaa
     5461 gcgcgagtgc cagcgccttg aaggccgcct cggcgcggtg gtggtcgttg tcgccctcga
     5521 ggacgtccac gtgcagggtg aggcgcgcag ttgaggccag gctcccaagg acgtgccgga
     5581 cgttctcgca ggcaagcccg ccaaggcgat cgcgctccag gccgagcctc acccgggcga
     5641 acgggcgccc cgagagatcg accaccgccc gggcgagcgc ctcgtccagc ggcgcgaacg
     5701 cccagccgaa gcgggcaatc cccgcacgcg tcccaagcgc ccgatccaac gcctcgccca
     5761 gcgcgagcgc gcaatcctcc gccgtgtgat ggtcgtccac ctggaggtcg ccctcgcacg
     5821 acaggtcgag gtcgaagcgg gcgtggagcg ccagcgtcgc gagcaggtga tcgaggaagc
     5881 cgacgccggt cttcactgcc gagcgtccct cgccgtcgag gcgcaacgcc agctcgatct
     5941 tcgtctcacg ggtctcacgc gacacgctcg ccttccggct catggcagca gctcctccag
     6001 ctcggcgaga tctccgagca ctcgtcccgc ccccgcctcg aacagcgcgc ccctcatcgt
     6061 ctcgggggcg tggccaggcg cgacgatccc gatcggcacc acgccggcgc tccgcgcagc
     6121 cctcatgtcg tcgggggtgt cgccgaccat ccacgcacgc ccgactccca ggcgctccag
     6181 cgcaagcctc acaggggccg catccggctt gagcggcccg tcctccatcg tcaccagcac
     6241 ggagaagcga tcgcgcaggc cgcagcggtc gaggaagcgg atggcgtcgc gccgcgggcg
     6301 cccggtaacg atgccgagtg gcaggcgggc ggccaggcgc tcgaggagcg gcggtgcagg
     6361 gatcaaccgc tcgcgctcgc agaggccggg cgcaccgggc gattccttcg atccctggta
     6421 gatctgctcg aacctcgcgg tgaccgcctc gagcggctgc tcgacgccgt gggccgccat
     6481 caggcggcgc gtcacctccc agtcgttgtt cgcgccgccg cgcgcgcggg ccgccgcgat
     6541 ctcgccgcga tcgatggccg ccccgaaggc ggcggccgtt tgcacgatcg cctcgtcgta
     6601 ggatgccgag acgtcggcga ccaccccgtc catgtcgaag agcagcgcct gcggctcgag
     6661 gactgccctg agcccccgtt cgagccgcgc gaaatcggcg gcgttccccg ggcaggcgat
     6721 gcgcagcgcg ttctcgaggt acgggcgccc cgggaagcac cggacaccga tgcccagccc
     6781 ggccagcgcc tcccacgtcc accgcacctt cgggaactct gcgagaacga agttggcctg
     6841 cgatggccag acacgggagg caagcggggc gaggaggcgt gccagcgcct cgcgctcgcg
     6901 acgcacctcg gccaccgctt cattgacacg ccgctcgtcc gaccggaacg acgccgccgc
     6961 caccgcgagc gacaggccgc tcaccgagta ggggttgccc gccgccctga gccagccgac
     7021 caggtccgct ggcccggccg cgcacccgac ccgcaggccc gcaaggccgc gcgccttcga
     7081 gaccgtccgc gtgagcatga cgttggggcg ctcgagcagc gcggaggtcg ggtcttcgtc
     7141 ggcgaactcg acgtacgcca ggtccaccag cacgaccacg tccggtgccc gcgcggacaa
     7201 ctccagcagc gccccggtgc tggcgcaggt gccggtcggg ttgttcggcg tcgcgatggc
     7261 gatgacgccc gtgcggcgcg tcacgcgaga gagcagcgcc tcgagcggca gcgggccgcc
     7321 ggtccacggc acgtccacgt accgcccgcc ggcaagcgca cagtaccgct cgaacatctc
     7381 gaaggtgggg gcggtggtga gcatctcgcg tccgggcccg acgaaggcgc ggatgatccg
     7441 atcgagcgcc tcgtcggcgc cggcggtgat gcgcacccgc tcgggcggca cgccgagccg
     7501 gtcggcccac agttgctgga gcgccgacgg atccgggtag cggctgatcg cggcggccag
     7561 ggggccggcc tcctcggcga gccgctcggc ctcgggcgcc ggcccctcgt tgccgtcgag
     7621 cgggaggtcg attggcgtgg gcagccgggg caccgcgtag ggcgtgatcg ctcgcagccg
     7681 cgcggttggt cgtctggtca tgctcgcacc tcacgccacg atgttcgagg gagacgtgac
     7741 cacgatgtcg gaaccgcccg cggccttgat gcggggaatg acggccgcca gttcgtcgcg
     7801 aagcacggcc gccttcaacg cgtagcccgc cccgccgtgc agcggcgcga tcgtcggctc
     7861 ccgcatgcac gggaggatct ggcagacggc ctcgaggcgc tcggccgaga cgttcacttc
     7921 gagcatgaca cgccgccggg cgtcgagcac cgaggtgagc agcagcgcga agcgctcgat
     7981 cgcctcccgg cgtccccgcg cctccaggct gcgggggtgc gcgaacagcc gggtcgtgga
     8041 cggcatcagc tcgtccacca ccaccagccc gttggcctcg agggtggcgc cggtagccgt
     8101 attgtccacg acgaggtcgg catcctcagg tggaaagacc tccgtcgcgc cgtaggaacg
     8161 caggaaccgc gcctgcaggc cgcggcgcgc gatccagccg cgcgacagcc gctcgtactc
     8221 ggacgcgacg accagcgggg tgtcgggcag ccgtccgtcc tggaccagcg cctcgggggc
     8281 cgcagccacc acccggaccg ggtcgaggcc ggtgtcgagg acctcgacca cctcggcacc
     8341 cagctcggcc acccagtcag cgccggcaaa gccgacgtcg cgggacccgg cgtgcagcat
     8401 ctcgacgatg ttctgcggct tgaggagctt cacctcgcag gagggcagcg agatggtcgg
     8461 gcggtagccc cgggtgccgg ccttcacgcg cagaccggcc gcggccagca gcgagaagac
     8521 ctcgtcctgc atccgccctt tcggcagggc caggcgaagc acgttcgggt cgacgatcgg
     8581 tgtcatgatt cccacaggcg atcaaacggc ccggggaacg cggcggggtg tcgtcgggcg
     8641 gtaacttcac ccgagacagc aacgccggcc accgggccgg cgttgtgatg gcgcgctcag
     8701 gaacagagcc tcatcagccg gccacgtgac cgtgggcgtg gtgatgatgg cggtgatggc
     8761 gcgcggcaaa ggtcatgaga ctctcactct ggcacgcccg ccgggacgtg tcaagcgttc
     8821 gagtgcaaca gcgtcgggcg cacaacgccg ttcggggcga accgcattcg ggaggaccgg
     8881 tgcggccccc gtcgtccggc gatggacggc gcggtcccag ctccggacac tggacttgaa
     8941 ccgtccgggt gtcgaggagc cgccgaaagc catgcggcac agcgatttgc cgtgcgcggc
     9001 gacgcttgcc ggaagcggca cggccttcgc tcatccgagg gcatgttcct gatcaccgac
     9061 ctgcgtcgcg ccgccgcaag cttcatgcgg acacccgggt ttgccgccgt ggcggtactc
     9121 gccctggcgc tcggcatcgg ggtcaacacc gccatcttca cggtcgtcag cggagtcttg
     9181 ctgcggccgc tgccctaccc cgaacccgag cggctggtcg cgatctacga gcgcgtcccg
     9241 gataaccccc ggctagcggt cgccccgccg agcatcgccg actgggccgg gaaccgggcg
     9301 ttcagctcgt gggccgtcac ggccggcgac nnnnnnnnnn gcaagttccg cttcaaacgt
     9361 gcggatgcca tgaaacggct ggaggcgacg gcggtgtcgg gcgacttctt ccagaccctg
     9421 caggtggcgc ccgagcgagg acggtggctg gaaccgcgcg acgaccaggc gggcgccgag
     9481 ccggtcgtgg tgctggggca cgcgctctgg aagcgctcgt tcggcggcga cccctcgatt
     9541 gtcggccggc aggtgaccct cacggacaag tcctacacgg tagccggcgt gatgcccccg
     9601 ggcttcgcgt ttcccgggga gacggaggcc tgggtcgcgc tgcagcttcc ggcggatcag
     9661 ctcctcgacg acaaccgcta ctcgcactat ctcgccgggg tcgggcgcct cgcgccgggc
     9721 gtcggcatcg agaaggcgtc ggcttcgctc aacgcgatcg ccgcgagggt cggcgcacgc
     9781 ttcggcgacg ggtacgcggg gcgcggcgtc aacctggtgc cgcttcgcgc cgacctcgtg
     9841 ggcgacgtgc ggccggcgct gctgctcctg ctcggcgccg tcggcttcgt gctgatcatc
     9901 gcctgcgtga acgtggcgaa cctgctgctg gcgcgctcgg ccgagcgcga ccgcgaggtc
     9961 gcgatccgcg cggccctcgg cgccagccag ctgcgcctgg cgtcgcactt cgcggccgag
    10021 gggctggtgc tcaccatcgc gggcgggctg gcaggccttc tcctggccgc ctgggcgagg
    10081 gacgcgatcc tgtggctggt gccggccgac ctgccgcgcc tcgatgaagt gagcctcgac
    10141 tggcgcgtgt tcgccttcgc cgcctctacc gccatcgtca ccgggctgct cctggggctt
    10201 gctcccgccg tcttcgcccg ccgccgcgcg cacgcgtcgc gactggcaag ccgggggacg
    10261 gcggggtcgc gcgagcgggc gcgcgtgcag agcgcgctga tgatcgcgga ggtggcgctg
    10321 tcgctgatcc ttctgctcgg tgcggggctc ctcgcaaata ccttctggcg cctgaccgcc
    10381 gtcgatcccg ggttcaaggc cgctgacgtc ctgaccttcg aggtcatgct gccgccgccg
    10441 gcgtacccgg ccgccgaagc gcgggcgcgc ttccaggagg acgtgctcgc gcggcttgcc
    10501 cggcttcccg gcgcgaggcg tgccggggcg gccaccaacc tgccgcttag cgggaccaac
    10561 atggcgttcg cgttctttta ccccggcgag agcatggaga cggccaagcc tcacccggcc
    10621 aactaccgcg cggtgagccc gggctacctg gaggcgatcg gcgtgccgct ggtcgcggga
    10681 cgctggctcg ccgagagcga ccgcgccgac cgggcgccag tcgtggtggt gaaccaggca
    10741 ttcgccaggc ggtatttccc gggtcgccag gcgatcggcg agcggatctc gatcacgcgc
    10801 gggcgcgacc tggtatggcg cgagatcgtg gggatcgtcg gcgaccttcg tcatcgcgcg
    10861 ctgtcggcgg acgccgaccc cgagatgtac gtcccgttcc aggacgaccc ggtgttcttc
    10921 atgcgcttcg ccctgaacgc ggcgaacgcc gagtcgctgg ccggcagcgc gagggcggct
    10981 gtctgggagg tcgacaggaa cctgcccgtg acgaagatgc ggccgctcga ggcgctggtg
    11041 gcggcgtcgg tggcgcagcc gcgcttccac gcgctgctgg tcggcgggtt cgcgctgctc
    11101 gcgctggtgc tcgccgcgat cggcctctac ggagtggtgg cgaggctcgt ggcgcagcgg
    11161 acgcaggaga tcggcgtgcg cagcgcgctc ggcgcctcgc cccggcagat cctcgcgctg
    11221 gtgggagacc acgtgtcgcg gctcgtgctg gcggggctgc tgatcggcct ggcgggcgcc
    11281 ctggcgctca cccgaacgct cgaacgcttc ctcttcgggg ttcagccgac cgacgtgtcg
    11341 accttcgccg cggtaaccgc gatcttcgct ctcgtggcgg gtgtggcagc gtggctgccc
    11401 gcgcggcgcg cactccgggt ggatccggtg gaggcgctcc gggcggagta accctcctcg
    11461 gctacctcgg ccgtttcgca cgcaggagga gcaccggtat tgaaacccgg tggcggacgg
    11521 cgtcggccgt ctggccgtgg atgatgtcgc tcacgccgcg gtgaccgtgc gtggccatcg
    11581 cgatcaggtc cacggcccgg tcgtgggcca cccggcagat ctcggaggcg ggatcgccca
    11641 tcgccaggaa cgactccacc tcgatccccc tccccctcaa ctgcccctcg agcgcctcga
    11701 ggtaggcacg gtcttcgcgc atctcctcgg actcccgcag gttcagctcg tcgaagtgcc
    11761 tcgcggccca gccgtcggca acatggacca gcaggagccg gctgcccgcg aggcgcgcca
    11821 gcttctcgac gtgctcgacg atcgtttcgt cggccacgct gtgctcgatc gccacgagga
    11881 tctgcctgta catccctcac accagcccgg caacgacctg ggccagcagc cagacgttca
    11941 aaccggcaat cacgacggcc gccggccagg cgagcagctt caggccgcgc ccgatcgcca
    12001 tcttccccat tatccggcgg tcgccggcga acgccaccag cgggaagatc gcgaacggca
    12061 gctgcaggct gaggatcacc tggctcagga tcagcagctc gcccgcaccc cggtcaccgt
    12121 acgcgatcac ggtcgccagc gcgggaacga tcgcgatgag gcgggtgacg aagcggcgca
    12181 gccacggccg gagccgcaaa ttgaggaacc cctccatgac gatctggccg gcgagcgtgc
    12241 cggtcagcgt cgcgttctgc cccgcgaaca gcagcgcgac ggcgaagagc gtggccgcca
    12301 ggctggtgcc gagcagcggc gccagcagct tgtaggcgtc cgagaggtcg gcgacgtcct
    12361 cgtagccggt gccgtggaag gtggccgcgg cgacgatgag gatggcgccg ttgatgaaga
    12421 gcgcggaggt gagcgcgacg gtcgagtcga gcgtcgcgaa ggacgccgcc tcggcccggc
    12481 ttgcctcgct gtcgccgtac ttccgcgtct gcacgatgga cgagtgcagg tacaggttgt
    12541 gcggcatcac cgtcgcgccc aggatgccga cggcaaggta gagcatctcc cgatcgcgga
    12601 gcagctcggg cgccggcatg aagccgccga gaacctgcag cgggtccggc ttcgagaaat
    12661 acagctcgag ggcgaacgcg ccgccgatgc cggcgatgag caggacgacc agggcctcga
    12721 cgtagcggaa ccggtgatgc tgcaggtaga ggacgacgag cacgtccagg gaggtgagac
    12781 agacgcccag ggccatgggc aggccgaaca ggaggtgcag cgcgatggcc gatccgatta
    12841 cctcggccag gtccgttgcc gcgatggcca cctcggccag cacccacagc gaaaccgccg
    12901 cccagcgcgg gtagcggtcg cggcacgcct gcgcgagatc gcgtccgctg gcgatcccca
    12961 gccgggcggc gagcgcctgg aggaggatcg ccagcaggtt cgagatgaaa atgaccgaca
    13021 ggagccggta gccgtagcgc gcgccccccg cgagatccgt ggcccagttc cccgggtcca
    13081 tgtaacccac ggcaacgagg tagccgggcc cggtgaaggc gaacagcttc cgccagaacc
    13141 ctgcaccctg cggcacgccg acggtgccgt gcacctcggg caggctggcc ggccgcccgt
    13201 ccatggcctt gcgagcctac gtcacttggc gacggtgaag gcggtgctcg ccttcgtcgt
    13261 cccccaggag acggccaacg tgccgccggt gggcgtgtcg tcgatcgaga tggtcagctg
    13321 ctcgacgggc gagtcgagct tctcgaccat catcggcacg cggccgagat cctcgccgga
    13381 cgggtagggc acgccccact gccccgtctt cttgctgacg atcagctgcc agtcgctctc
    13441 gcctggcacc gtgtaaaggg tgtaggtgcc ggccggcacc tgcaacgtgc cgaaggtgag
    13501 cgccttgtcg gtctcgagcg tggtggcctc gtccgcgccc gtgcgccact ccttgccgaa
    13561 gggggccact tccttgccga ccgtgcgccc cttcagcgac gggcgtccgt aggtgatcgc
    13621 gatggtggcg ccgtccactg tccactgcga tttcacgtgc gggctgcccc ccttgccggg
    13681 gtgcacctgg gtggtctgct gcgcgcccgc cgccgcggcg aaggcgagcg cgatcgccgc
    13741 cgtggcggcc gtgtgtacca agcgtctcat gcgtcctcct ctacacatcg taggggcgac
    13801 ccttgcggtc gtccctgccc ctaactccca atcctcgctc gtgcaatccg gtccaccccg
    13861 ctgcaggcca gccacagctc gtcgttcggc gcggccacca tgtggcgcac cacgccgccg
    13921 cccgacggga ttggccacgc ctgcatcttc ccggtggccg ggtcgaagcg caccagcgtg
    13981 ttcggctcga cgcccgattc gctgaaccac acggcgccgt cgctcgtgac ggccatgccg
    14041 taggggcggg agccggaacc gcccggcgtc gggaactcgg tcaccttgcc cgtcgccggg
    14101 tcgagccggc cgagcatccc gcgcgcgtag tcggtgtagt acaccgtgtc gtccttgccg
    14161 atggcgatgc ggcgaggccg cgcacctggc gagggcagct cgtactcccg aatggccagc
    14221 gtgctgggat cgatgcggcc gatcttgttg gtgttgaaca ggtcgaagta cggcacgccg
    14281 cgcgagtcga cgacgatgcc gtacgggcgg gagtcgggcg tgggggcggg aacgagatcc
    14341 accttgccgg ttctcgggtc gaggcggccg aggaagttgc tgccctggac cgtgaaccac
    14401 agcgtcccgg cctggtcgaa gatcagcgtg tgcgggtcgc gggccttcgg gtcgggcatc
    14461 ttgtactcgg tgaccttgcc ggtcttggga tcgagcttgc cgatcaggcc cgagtagttg
    14521 cccgtgtacc agatgttgcc ctcgcggtcg gcgacgatgc cgtgcggccc ggtgttctcc
    14581 gtcgggagct tgtattccct gatcttgccc gtggcgggat ccagccggcc cagcaggttc
    14641 gcccgcatgc ccgtgtacca ggcactgccg tcgggcgcga ccgccggatc gtgcgggaag
    14701 ctctggctgg tgggtgtcgg ccactcctgg aacgccacgt ccaccggcga ggcggctggc
    14761 tgggctggct ggccggcggc gagtgcgacg ccggcggcgg ccgcgagcag cgccgcgcag
    14821 gcggacgccg ttcgggttga gggacgcatg ggggcctcct gctgttgatt ccctgtcttt
    14881 cagtatcgca tcatgggggt cggggataat caggattcgg gattcaggaa tcggattcgg
    14941 ggagggaccg tgcggcctct gttggcactg gccggcctgc tcgttgcgct gccggtggtt
    15001 gcggcgtacc agaacggccc gatgcccggc gtcaccggcg gcttcggcga gccgacctgc
    15061 acgatgtgcc accaggggcc gcctcccggg ccgaaggacg gcaggctgtc gctggaggcg
    15121 ccgcgtgagt tccgtcccgg ccagacctac cagcttcgcg tcacgctgac ccggcgaaac
    15181 ctggccgtgg gcgggttcga ggtcgcggcc cgcttccaga cgcgccccgc ggcgggcgcg
    15241 caggcgggaa cgctccggtc gctcgatcca cgcacgcaag ttgtgacggg agccaagggg
    15301 gtgcagttcg cccagcacac ccaggcgggg tcgaaggccg tccctccagg caagatgacg
    15361 tgggtcgtcg agtggcgcgc acccgagcag gcgccgggca acgtcctctt ccacgttgcc
    15421 gcgaacgccg ccagcggcga cgcgtcgccc ctcggcgacc tcatacacac cctcgaggtc
    15481 gtctcacgcc cagcgcccgc ggcacgcgcg ggctcgcccg cccccgtttc cgcacactga
    15541 tcggacgact tcaccacgca cagtgcatgc ggaaatctcg gatcgttaca ggagatctct
    15601 gcgccctctg tgcctctgtg gtgacccgta cgtggcagac gtggcggctt gtccacgttc
    15661 aacgcagagg cgcggagacg cggaggtgca cgcggaaatc tcggatcgct acaggagatc
    15721 tctgcgccct ctgtgcctct gtggtgagtg gtgaaccgta cgtggcagac gctcaggccc
    15781 gagtcgccgg aggcggccct actcggccgg cacggaggcc ggccctactg ggggtcgctt
    15841 ctggacggcg tcgcggacca tctcggtgat cgtgcggaag tcgagcttcc cgctgctcat
    15901 cttcggcagc tcgggcagga cgacgaactg cttcggcagc gcgatccgcg gcagcgactc
    15961 ggccatctgc gcgaggatct ccttctcgtc caccgacgcc gtcacggccg cgacgatgcg
    16021 cgcgccccgc gccgcgtcgg gcacctcgac gacgcagcac tggcagtcgt cggggagcag
    16081 tctgtcgagc acgtcctcga cacgcacgag cgacaccatc tcgccgccaa ccttcaggaa
    16141 ccggcgcagc cgccccacgt gccacaggta gcggtcgtcg tccatgtagc ccatgtcgcc
    16201 cgtgtcgtac cagccgtggc gcaggtggag cgaggtggcc tcgaagtcgt cgaagtaccc
    16261 gttcatcacg ctcggcccct tcaccagcac cttgccgatc tggttgaccg cgcactcctc
    16321 gcccgtctcg tagtgctcga gccgcacctc gacgttcggc agcggcctcc ccaccgaccc
    16381 cgggcggttg tgctgcgggg cgttgaccga gatgaccggg ctcgtctcgg tggtgccgta
    16441 gccttcgagg aggatgatcc ggtgcttctc ccaccacccg cggcgcagcg cctcggtgca
    16501 cttgtcggcg cccgtgatga gcagccggat cgtggcgaag tcacccggct cggacttctg
    16561 caggtagccg ttgaggaacg cgggcgtgcc caccatcacc gtggccttct cgtcgcgcac
    16621 tgcctggcag accgaccgga agtcgagcgg gttcgggtag gtgacgatcg tcatcccctg
    16681 ccagagcggc agccacatgt tcacggtgag gccgaagacg tggaagaacg gcaggttcgc
    16741 catcatcacg tcctcggcgc tgaagtcgac gacttgcgcc acgccctcga ggttggccgc
    16801 gatgttcgcg tgcatcaacg gcaccgcctt cggttcccgc tcgctgccgc tggtgaacag
    16861 cacgaccgcg tagtcctcgg cgtccccctt cgggatcttc ctgatgatgc ggtcggccga
    16921 gcgggcggcg cgggccgccc ccttcagctt gtcgagcagc gaaaccccgg cagcgaggtc
    16981 ctcgaggcag accatcccgt cgatgagcgg gcagttgatc cgggcgagca gcgccttcga
    17041 ggtgaggatg gtgctgaagg cgaggcggcg ctgcgcgtcg cggcagttgt cggcggcccc
    17101 ggtcgaatag ttgatcatca ccggcgtgcg gccgctcaac agcacggcca gggtcgcgta
    17161 catgcagccc gcgctggtgg gcaccatgac gcccacgaac ccgccggcct cgcgcccgtc
    17221 cagcttcgcg gccaggatga gcgcgcgcag cagcacgtcc ttgtaggtga cccgctgccc
    17281 ggtcgcccgg tccgcgacgg ccagcttctt cgcgtgcttc ttggcgctcc ggacgaacat
    17341 ctcgtgtaaa cgcacgcgcc cctcccgcgc acattctacc ggcaccgccg tgtatgatca
    17401 cctgcatccc gcgcccccct ccgatccagc agccccgtgg agcgtcgcgc atgcccagcc
    17461 ggcccggccg cctcttcctc atccttgcct tcgtcgcggg cgccagcccc tggtggccgg
    17521 ccgccgatgc cgccgcacag ggagccgcgc cgccacaagc agcgccgtcg caggcagggc
    17581 cggcggcccc cgggcggccg atcaccgctg acgactacgc gcggggcgaa cggttcctcc
    17641 cgcagaacgt tgagccgctg gtgatcggag gacgggtgac ggccaactgg ctgcccggcg
    17701 accgcttctg gtaccgcaac gacctcgccg atggccacga gttcgtgctc gtggatccgg
    17761 ccgcgcgccg ccgggcgcgt gccttcgacc atgcccggct ggccgcggcc ctctcggccg
    17821 cttccggccg cgcctacgcc ccgaacgccc tccccttcca gtggatcggg atctccgcgg
    17881 acggccggca aatcaccttc gatctcgagg gccgccggtg gtcgtgcgac gcgctcggct
    17941 cgaggtgcgc cgacacgggc gcctcgccgg agccggcgcc ggtgggcgcc gctcccgcgc
    18001 gccgcggccc gcgcacgaag gccgcgagcc tgccgtcggg cgacgggaac ctcagggtgc
    18061 gcgagctggc caccgggcgc gagcgccagc tcaccaccga cggcgcgccc tacttcggct
    18121 acgcgaccga caacgccggc tggcgatcga gcgaccgccc gatcgtcctc tggtcgcccg
    18181 actcgagacg catcgccacc taccagcagg acgagcgcga ggtcggggag atgtacctcg
    18241 tcaacacgcc gatcgacccg gtcacgcacc ccaggctccg cgtctcgaag ttcccgctcc
    18301 ccggcgaccg gcacgtggcc atgctccacc gtgtggtgat cgacgtcgag acgggccgcg
    18361 tgacccgcct cgcgatgccg cccgactacc accgcgccac gctcggcgac gacgtgagcc
    18421 tggacgactg gcagtggaag cccgacggct cggcgttcgc gttcatctcc acctctcgcg
    18481 accacaagga ggcggtgctg cgggtggcgg acaccgcgac cggccaggtt cgaaccgtga
    18541 tgagcgagaa ggtggccacc cactacgagt cgcgggtggg ctgccgcgtg ctgtgggaca
    18601 gcaacgaggt gatctggtat tcggagcgcg acgactgggg ccacctctat ctctacgacc
    18661 ttgccacggg ggcgctcatc cgccggatca ctgcgggcga gtggccggtg atggcgctct
    18721 tgcgcgtgga cgagaaggcc cgcacgctgt ggttcgaggc gcagggccgg gaaaagggcg
    18781 aggacccgta cttccggcac gtctaccgca tcgggctcga cggcacgggc cttacgccgc
    18841 tgacgcctgt cgagggtgac cactcggtcc agctgtcgcc gtcaggccgt tacctcgtgg
    18901 acacctactc gcggcccggc gtcgagccgg tggtagccct gcgcgaaggc gagggccgct
    18961 tcgtgatgat gctcgaaaag gccgacatca cgaggctgcg ggccgcgggg tggcggcccc
    19021 cgattcccat caccgtgaag gcccgcgacg ggaagaccga ccttcacggc ctgatgttcc
    19081 ggccaacccg cttcgacccg gcgcgcaagt acccggtgat caactacgtc tatccggggc
    19141 cgcagaccgg cagcaccggc agccgctcct tctcggcggc ccgcggcgac aggcaggccc
    19201 tcgccgagct cggcttcatc gtcgtcacca tcgacgggat ggggacgccg gggcgatcga
    19261 agtcgttcca ggacgcctac tacggcgcga tggggcgcga caacacgatc cccgaccaga
    19321 ttgccggcat gaaggagctg gccgcgcggc acccgtggtt cgacgtctcg cgggctggga
    19381 tctggggcca ctcgggcggc gggttcgcga cgaccacggc gatgttccgg ttccccggct
    19441 tcttcaaggc cgggatcgcc gagtcgggga atcacgacca gcggctgaac gaggacgact
    19501 ggggcgagcg ctaccaggga ctgctggtgc gcgacgggca aggtggcgac aactacgcgg
    19561 ctgaggcgaa ccagctctac gcgcagaacc tcgaggggcg tctgatgctg gcgcacggca
    19621 cgatggacac caacgtgccg ccgtaccaga cgctgatggt ggccgacgcg ctgatcaagg
    19681 cgaacaagga cttcgacctc gtgctgctgc cgaaccagga ccacggctac ggcgacgcgt
    19741 cggcgtacat gatgcggcgg cgctgggact tcttcgtgaa gtggttgatg gggacggagc
    19801 ctcccaagga gtacaggttc ggccggcagg ggcccggccc cgctcgacgg gcgccggggg
    19861 agttgcgctt cggccctgcc cggcgcgagc ccacccgcgc cgacgtcctg cgcggcaact
    19921 gggaccgcta ccgctcgaac aacgacctgt tgttctacca cctcgacgtc cgggtcgacc
    19981 cggagcgcaa gtggatcggc gggaagaaca ccatccgttt ccggatgacg cgcgacgaca
    20041 cgaggatcca gattgacctc gcgaaggcgc tggcggtgga ccggatcgtg ttcggcggac
    20101 gcgagctgcg gtacgagcgc gaggaagggg cggtgttcat cgacttcccc gagacccttc
    20161 gccgcggccg cgagtact
//
LOCUS       DAOPQX010000024        19442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3431_length_19442_cov_4.648321, whole genome shotgun sequence.
ACCESSION   DAOPQX010000024 DAOPQX010000000
VERSION     DAOPQX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 19442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19442
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_19442_cov_4.648321"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="PKK95_02480"
     CDS             <1..1428
                     /locus_tag="PKK95_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC17101.1"
                     /translation="RDPQRRARAARRLGAARRPSNVTDLEPLLGDPEVPVRLAAIEAL
                     GEIGGEAAAGPLIAAARGADAVRPESEAAGMRAAVVAALTSAGEAAVPALLDAAGDRH
                     ARVRECAVAALAAIGGEQAAGGLARALRDDRSALRQLAAHALARVSGGRAIADLANAL
                     LHKDAATRRAAAEALGETTAEEAVPALQRALADTDRGVQAAAVGALARIGRGPAIEAL
                     VGALVRPDRELRLSAAQALRSAGWVPEGPGPRTLHAALHGRFDEIGTAAPGDAVFGGA
                     TIEMLKAALSDKNPATRAGAAAALGRMGDQAAAAALGSVLGDPDPAVREAAAASLAQL
                     GPPAFDAILPGLEDRSQTTRAAAGRVLQALDAGRVADFLVDRLSPGEAVTHGGLPLRI
                     VDRLEALDAARHAADSLERLLRVAGSGVPPASVQRAAALADLVLIEPGQVPDAGERLD
                     LEDLRELAARTLSGTVPNPARGARR"
     gene            1425..2513
                     /gene="rsgA"
                     /locus_tag="PKK95_02485"
     CDS             1425..2513
                     /gene="rsgA"
                     /locus_tag="PKK95_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOC17102.1"
                     /translation="MSAAPTTAWSLSELGWSERFAQAFEPLARPNVIPGRVTLEHKHI
                     YRVLTEHGERLARAAGRLRHRASAPVEYPAVGDWVALRLNPGELRGSILQILPRFSRF
                     SRKVPGELTQEQVVAANIDTVFLAMGLDADYNLRRIERYLVTGWRSGAQPVVLLTKAD
                     LCDDVEARVREVEALSAGAPVHATTAMSEGGVDIVRRYLEPGRTVAILGSSGVGKSTL
                     INRLAGKELLRTGDLTADARRGRHTTTHREMIVLPGGGLIIDTPGLRELQLWEAGDSL
                     GSAFPDIAALAEGCHFADCAHDTEPRCAVRRAVEEGRLAPDRLDGYLKLQAEARRFDE
                     KQDELAILERKRKAKILGRIIKRMPKKG"
     gene            complement(2674..4026)
                     /gene="rmuC"
                     /locus_tag="PKK95_02490"
     CDS             complement(2674..4026)
                     /gene="rmuC"
                     /locus_tag="PKK95_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOC17103.1"
                     /translation="MIETLLVVAVVLLFILLALTVVLLLRRGPAELAAIASRVEGLAA
                     DHERMERSVREEMTRGRSDAAAQARALREEVAGALDRLGESLRQGQSMMARGQGEQLA
                     AFAQQLTALGEGTERRLEALRGTVADQLRAIQDDNAAQLEKMRETVDERLQGTLEARL
                     GESFRAVSERLEQVQRGLGEMQALASGVGDLKRVLTNVKTRGTWGEVQLGTLLEQALS
                     PSQFATNVETREHSGERVEFAVRLPGKDPDRAPVWLPIDAKFPQEDYQRLIDAAERGD
                     AEAVEGAARQLENRVKVCAREIHDKYLDPPNTTDFGILFLPTEGLYAEVLRRPGLAEI
                     LQRDHRVLVAGPTTLWAILNSLQMGFRTLAIERRSSEVWTVLAAVKTHFGRFGELLGS
                     VQKKLQEATNKMDAVSRQSRTIERKLRDVQELPANRSAEDLLLAAPLDLLDDDEPAEV
                     "
     gene            4092..5315
                     /locus_tag="PKK95_02495"
     CDS             4092..5315
                     /locus_tag="PKK95_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOC17104.1"
                     /translation="MPTRACLAVLVLLLAAPRAGADNKILHLAIGDPARRDREAPLVL
                     DAVTDTATGEVVTPEDLPPRLAKTGLLLLGEEHTTMETHQVQLRILRALADSGRRVLI
                     GLEMFPYTAQAALDEWHAGSLPEEEFVRKSRWYEHWGYNWRYYRDILLFARDRKIPMF
                     AVNTPREVVASVRKKGLANLSPEEAAHIPDRIDADNADHLLYFKTTFEGEEGPVHGAG
                     MSDEMWKNMLSAQATWDATMGYNAVQAWKRANDLGAIMVVLVGLGHVAYGLGIERQAR
                     NWFDGQIASVIPVTVNGPDGAVASVRASYANFLWGLPHAIDSLYPSLGLSTAVGQGET
                     ARRVILVQKDSVAERAGVKPGDVIVSVDGTPLSDKEAYNRLMAEKRWGDRARIVVRRG
                     GEDVTLTALFRRTVK"
     gene            complement(5331..7031)
                     /locus_tag="PKK95_02500"
     CDS             complement(5331..7031)
                     /locus_tag="PKK95_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOC17105.1"
                     /translation="MRYVALACDYDGTLARHGRVDEPTLAALRRVRDSGRRLILVTGR
                     RLDDLQSVFPEYQLFDRIVAENGGVSCDPARRETRRLAEPPPPAFAEALRERGVTPFD
                     TGEVIVATWRPNELAVLGAIRDLGLELQVIFNKDAVMVLPPGINKASGLLAVLAEMSL
                     SPHNVVAVGDAENDHALLQSCECGAAVANALPMLKERADLVTSADHGAGVVELVERLL
                     ANDLDDLAASLRRHEILLGRDGSGSEVCLPPYGRSALLAGASGGGKSTLASAFLERLA
                     ERGYQFCLVDPEGDYETFERGVVLGTSRKTPEPGEVMTVLDTPAQNVVVNLTDVARDE
                     RPRFFEALLPRLQELRSATGRPHWIVLDEAHHLLPRDRRSSGSIVPQRLRGALLITLE
                     PSSVAREVLQSIDTLLVLGEAAGVALADFASAVGDEGAAPDVPRLEPGDVAMWERGSS
                     RARTFTVEPPRNTHRRHRRKYAEGELIEDEHFAFRGPEGKLNLRAENLRSFVKIAEGV
                     DEETWLHHFRRGDFSRWFRLVIKDEELAREAEEAEGAGLPAPEGRARIREMVERRYAV
                     "
     gene            7151..7876
                     /locus_tag="PKK95_02505"
     CDS             7151..7876
                     /locus_tag="PKK95_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOC17106.1"
                     /translation="MIERFRNPSPRIRRALDIAGIAHDGAFRKGTDVPYIHHPVAVAV
                     ILEAYGYPEPLVAAGLLHDTVEDTRWGNAEVQRRLNQVTEGRLPSPAEPMAFRSAFCQ
                     YLRDEFGREVFGLVAAVTEKKNDGGVPADWLERKKNQLDKLANAGPDEAALKAADAVH
                     NIESTARDIEALGLGVLDRFRGGPLVVWHYSALASLASRRMPAGAPLAERVVRAAFDL
                     ESAVRRRRPVRDGSAPYPEPVVI"
     gene            complement(7882..8826)
                     /locus_tag="PKK95_02510"
     CDS             complement(7882..8826)
                     /locus_tag="PKK95_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOC17107.1"
                     /translation="MFETVRIGVSSCLMGRKVRYDGGHKRDAFLVETFGKFVEWVEVC
                     PEVEVGLGTPREAIRLVRDGDQVRLVGVESATDHTDRMRRWAAARVESLAGEALDGYI
                     LKKDSPSCGMEHVKVYTLGGMPSREGRGLFADALISRLPLLPVEEEGQLADPRLREHF
                     VERVFAHHRLRAFFSGRWTAGSLVAFHAAQKLSLLAHSPSGYAALGRLVARAGDMPRA
                     ELRAAYETGYMRAIAVVATPSRHVNVLQHMAGYFRKAVDEASRQELAQAIADYQQGLV
                     PLIVPITLVRHLVRRCGIEYLAGQVYLEPHPKELMLEP"
     gene            9084..11648
                     /locus_tag="PKK95_02515"
     CDS             9084..11648
                     /locus_tag="PKK95_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17108.1"
                     /translation="MIRPRLALVLGLWLTAAPAPSAAVPGQAGPAAEPVAIPRFERGA
                     NPIALGGPARPGRYMEASGLRAAFLGREDGSFEAWAYPLKLLHDFNLSFGIAAYADPI
                     PGAALASTVDIRPESATVRYAHAAFTVDAIWLVPRDEPGGLVLLDVRTSEPVTVVVRF
                     RPDLKPMWPAALGGQYSYWDDTLKAFVVGEGSRKNAALIGSPFGLTPPEQPAHNLPDA
                     PSQFTIRVTPEQARKGLIPIAMAGSVEKLDAAKQTYQALLASAERLYREQEEHYRRLR
                     EERTSVETPDPRIGLAFEWAKVALDKGFVCNPHLGCGLIAGLGPSGTTERPGFGWFFG
                     GDTFINNWAISAYGDLDTVRRSLDFLRERQRADGKMMHELSQGAGYIRWFQDYPYGYY
                     HADTTPLYIISVRDYVRATGDAAFARDYWPSMRRAWDYCVSTDEDGDGLMDNTKAGLV
                     IETGALRSADVQTDVFLATVWTEAAAAMIDLARLAEPGFVSTAEAAADKARAAINRRF
                     LDGARRRINFAILKSGAGQAEETDWPAFGIWRGVFEPGHPAIAGMLDELARSGLATDW
                     GARMLSRESALYEPLSYNNGASWPFLSGWAALALYTGGRPEAGWQYVDALADLTFLEA
                     RGFIPELLSGDRLRSIDAAVPHQLFATTGFATALMRGVVGLEETDQGLRLTPRLPAGW
                     PFLRVKRLRFRDGVGDVEIRRRPDGYAISVSNLRKPLAVGVRIALPPGSEVEGGGQAE
                     MPLATVPAPKTVTLRVRPGVELAPVQRPLALGDESHRLRVIETSVAAGVFRARLQGLR
                     GHTYRLRLDVPFEVLAIEGASEVGRDGASRLVEVTIPDGPGEWVETTLTVGLGRRR"
     gene            11802..12854
                     /locus_tag="PKK95_02520"
     CDS             11802..12854
                     /locus_tag="PKK95_02520"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOC17109.1"
                     /translation="MQKAPFGTTKEGVAVDLYTLTNHHGMVAKVITYGATLTELHVGD
                     ASGKPVNIVLGYDRLEPYLAGVPYFGATVGRVGNRIARAEFTLDGKAYKLAANDGPNH
                     LHGGLKGFDKVVWRAEEVKSPAGPAVKFTYRSPDGEEGYPGTLDVSVTYTLTDANELR
                     LDYRAVTDKPTPVNLTNHSYFNLAGDGSGDILGHVVTIAADGMTKVNERLIPTGEIVP
                     VKGTPWDFNVPTAIGARIKQVPGGPPVGYDHNYVLRKADPGGLPELAARVVEPKSGRT
                     MEVLTTEPGMQFYTGNFLDGSLKNRIGVPYKQYSAFCMETQHFPDAVHHAAFPSTILR
                     PGSEFTSTTIYRFGHE"
     gene            12873..14639
                     /locus_tag="PKK95_02525"
     CDS             12873..14639
                     /locus_tag="PKK95_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC17110.1"
                     /translation="MAANWLGLRWASGARPQGNARHARLVERRLSTARVVLAVASVAA
                     LVVEHRARLDPLGASVLAAFALYSAVLALELRRESDDAAPPVLGLHAADFAWVLAVFS
                     TNAGVNGPFLGYFLFVLLAAGSRFGLLETALTGAAATAGTVADAAARTWLLPAVAVDW
                     HIVAIDLVYLVVGTWLVAYLAEYERQHRQQTWATAEILGRIRPHQGLVASVQNVLDGL
                     LRQLSARRAMLVIADLDSDRVLLWEARADESGGRATIRLRQEMRASCGTWLFEVPARA
                     DAWRGVRRRHASPGAVVPAIDSRGDRFAAPLSLAPLLDAPFEWDEVLCLTTLVGEGWQ
                     GRLFLFDPRIWRPGEQNLVFLQVVMGQVGPALFNLYLQRRLRSRSGALERADISRQLH
                     DGVIQSLIGIEMQIDVLRRQAGADAGMAADLNGIQRVLVDEILNLRDLMQVLKPADVG
                     PAQLAEHLAGEVAHFRSRTHIDARLSCTEEEIDLSPRACREIAAIVQEALANVRKHSG
                     ATAVMVRLSRHDAGWLLTVDDNGHGFAFDGAMDGAELEARHLAPVIIGERVRAIGGRL
                     TVSSQRGTGSRLDISFPGRHHA"
     gene            14632..15300
                     /locus_tag="PKK95_02530"
     CDS             14632..15300
                     /locus_tag="PKK95_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC17111.1"
                     /translation="MPDRERRTRIVIADDHPIFREGLRRLLEAEPRFQVAGQAHNGLE
                     AVRLVEELRPDVLLLDLAMPGAGGIQALQEIADRCLDVKVILLTAAIDTADSVRVLQL
                     GARGVIMKECATHLLYKCLDTVTSGGYWVGHDRVNDIVQHLRAGATRPPAPAAMLTRR
                     ELQIVTAILNGATNKDIGQQFNLSEQTVKNHLSHIFDKVGVSNRLELALYALHHKLVE
                     GARK"
     gene            15724..17739
                     /locus_tag="PKK95_02535"
     CDS             15724..17739
                     /locus_tag="PKK95_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HOC17112.1"
                     /translation="MRAAIPGGSLDPGLISKYQTPLLIPPVMPRAGSIQMRGGKTGDY
                     YEISVRQFQQQILPAGLPATTVWGYGPVKSESKKGLLLHNAPSLTIEASHNRPVRVKW
                     INELTDGNGNYLPHLLPVDPTLHWANPPGGPTGRDTRPDFSGKAYVRPNEFNDPETQY
                     TSYQGPVPLVTHLHGAVGVGDESDGYAEAWFLPAAKNIPAGHAIEGTWYDFFRRKAAA
                     YGATWGPGFATFQYPNENRASTIWYHDHALGMTRLNVYAGPAGFYIVRGGPAGDKAVL
                     DSRTGLSATLPGPAPQEGDKFPANKPYREIPIAIQDRAFNSDGSLFYPDTRAFFDEYD
                     GPSIPDTDISPIWNPEFFGNCMMVNGNTWPFLNVEQRRYRFRFLNGCQSRFLILDFAS
                     IPGVEVWQIGNEGGFLGAPVNVTADGNNRLLMALAERADVIVDFTNVSPGNYVLGNLG
                     PDEPFGGGEPGIDFDAADPSSTGQVMEFRVGPALTVDKTTPAPFLVLPPVTPLPAEVR
                     TRPLALIEMMSMHYDGPAEAMLGTVGSDGMPAHSMWMDPVTENPAVGDTEVWELYNFT
                     ADAHPMHVHEVVFEVVNRQALETNADGETALPVSLVGEPRPPEPWETGFKDTVMAYPG
                     EVTRIRATFSKGGRFVWHCHIVEHEDNEMMRPYRIGPVQPGEPGLPE"
     gene            18131..18481
                     /locus_tag="PKK95_02540"
     CDS             18131..18481
                     /locus_tag="PKK95_02540"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOC17113.1"
                     /translation="MEQLLERPRAARYEMSAPVMYRQVGEDSWHEGRTLNVSSSGVLF
                     TCPAPALEPGTKVEFLLVLPNPGLREQPRVQCRGAIVRHERRRAGPACAVAATIDAYV
                     IVPAGQPSAGRVDA"
     gene            18511..>19442
                     /locus_tag="PKK95_02545"
     CDS             18511..>19442
                     /locus_tag="PKK95_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17114.1"
                     /translation="MSRSSLRIAFGLILAVAVAWGMSPDPRAAAGAAANAGASAVTWG
                     GTIASTVASAALPGTPAGEPEPIPSRSLAGAITDDAAANDAPQGNGKQGKGQAKPKGK
                     PTQEDFYAKFQKVSPAEQKAAAKRSAEMGMLPGAAGLELAAASITPADLPVLEGPGGV
                     PHYFGPYGNWAYSPLPSFPILSVSVDAGGSGYSAPEVFIKDVYGTGSDSADAPLMGTA
                     TVTDGVITEIAIPEGGKGFHVPFVEIIDPTGTGAEATAVLNLERPSGGIRKFVDRLPG
                     LGPTGANNIIQAHGLGQYLPVAAKEEWCSRPEAIA"
BASE COUNT         2928 a         6978 c         6808 g         2728 t
ORIGIN      
        1 cgcgaccccc agaggcgggc ccgcgcggcc cggcggctgg gcgccgcgcg ccgcccctcg
       61 aacgtcacgg acctcgagcc gctgctcggc gacccggagg tccccgtgcg gctcgcggcg
      121 atcgaggcgc tcggcgagat agggggagag gcggccgcgg ggccgctgat agcggcggcg
      181 cgaggcgctg acgccgtgcg ccccgaatcg gaggcggcgg ggatgcgcgc cgcggttgtt
      241 gcagcgctga cctcggccgg agaggccgcg gtgcccgcgc tgctcgacgc cgcgggcgac
      301 cgtcacgccc gggtgcgcga gtgcgcggtc gcggcgctcg cagccatcgg cggcgagcag
      361 gcggcgggcg ggctggcgcg tgccctccgt gacgaccgct cggccctccg gcagctcgcg
      421 gcgcatgcgc tcgcccgcgt gtcgggcggt cgtgcgatcg ccgatctcgc gaacgccctc
      481 ctccacaagg acgcggcgac caggcgggcg gccgccgagg cgcttgggga gaccaccgcc
      541 gaggaggcgg ttccggcgct ccagcgagcg ctcgccgaca cggaccgcgg cgtgcaggcg
      601 gccgccgtcg gagcgctggc ccgcatcggc cgggggcctg ccatcgaagc gctggtcggc
      661 gcgctcgtcc ggcccgaccg cgagctgcgg ctctcggccg cgcaggccct gcgctctgcc
      721 ggctgggtgc ccgaggggcc aggaccgcgc acgctccacg cggccctgca cggacgcttc
      781 gacgaaatcg gcacggccgc gcccggcgac gcggtcttcg gcggggccac catcgagatg
      841 ctgaaggcgg cgctgtcgga caagaacccg gccacgcgcg cgggcgcggc ggccgcgctc
      901 gggcggatgg gagaccaggc cgcggcggcg gccctcggca gcgtgctggg cgaccccgac
      961 cccgccgtcc gcgaggcggc cgcggcgtcg ctggcgcagc tcgggcctcc cgcgttcgac
     1021 gccatcctcc ccggcctcga ggatcgcagc caaacgaccc gcgcggcggc gggccgcgtg
     1081 ctccaggcgc tcgatgccgg ccgggtggcc gatttcctcg tggatcgcct gtcgccggga
     1141 gaggcggtga cccacggcgg cctgccgctg aggatcgtgg accggctcga ggcgctcgac
     1201 gccgcacgcc acgccgcaga ctcgctggag cggttgctgc gcgtggccgg cagcggggtg
     1261 ccgcccgcca gcgtgcaacg cgccgccgcg ctcgccgacc tcgtgctgat cgagccgggc
     1321 caggtgcccg atgccggcga gcggctcgac ctcgaagacc tgcgtgagct ggccgcgcgc
     1381 acgctgagcg gcaccgtccc gaatcccgcg cggggggcga ggcggtgagc gccgccccca
     1441 cgacggcctg gagcctcagc gagctgggct ggtcagagcg cttcgcgcag gccttcgagc
     1501 cgctcgcccg gcccaacgtg attcctggtc gtgtcacgct cgagcacaag cacatctacc
     1561 gcgtcctcac cgagcacggc gaacggctgg cgcgcgcggc cgggcgcctg cggcatcgcg
     1621 cctcggcccc cgtcgagtac ccggccgtcg gcgactgggt ggccctgcgc ctgaacccgg
     1681 gcgagctgcg gggctcgatc ctccagatcc tcccgaggtt cagccgcttc tcgcggaaag
     1741 tgccggggga gctcacgcag gaacaggtcg tggcggccaa catcgacacg gtcttcctgg
     1801 cgatggggct ggacgccgac tacaacctgc gccggatcga gcgatacctg gtgaccgggt
     1861 ggaggagcgg cgcgcagccg gtggtgctgc tgaccaaggc cgatctctgc gacgacgtgg
     1921 aggcgcgggt gcgcgaggtg gaagcccttt cggccggagc accggtgcac gccactaccg
     1981 cgatgtccga agggggcgtc gacatcgtcc ggcggtacct cgagccgggg cgcacggtgg
     2041 ccatcctcgg gtcgtccggt gtgggcaagt cgaccctcat caaccgcctg gccggcaagg
     2101 agctgctgcg gacgggcgac ctcaccgccg acgcgcggcg cggccgccac accaccacgc
     2161 atcgggagat gatcgtcctg ccgggcggcg gcctcatcat cgacacgccg ggcctgcgcg
     2221 agcttcagct gtgggaggcc ggcgactcgc tcggaagcgc cttccccgac atcgccgccc
     2281 tggccgaggg gtgccacttc gccgactgcg cgcacgacac cgagccgcgg tgcgccgtgc
     2341 ggcgggccgt cgaggagggc cgcctggcac ccgatcggct cgatggctac ctgaagctgc
     2401 aggccgaggc ccgccgcttc gacgagaagc aggacgagct ggcgatcctc gagaggaagc
     2461 gcaaggcgaa gatcctgggc cgcatcatca agcgaatgcc caaaaaaggc tgacagctca
     2521 cagctgacag ccgacagccg acggcggcct gctgtcgctt gcaggaccat ccttcggcag
     2581 ggctcagggt ggccctgagc caagtcgcag ggccacaggg gtcgcgccca cgctgcctgc
     2641 tgtctgctgt ccgctgtctg ctgtctttct ctctcacacc tcggctggtt cgtcgtcgtc
     2701 gaggaggtcg agcggggcgg cgagcagcag gtcctcggcg gagcgattgg cgggcagctc
     2761 ctgcacgtcg cgcagcttcc gctcgatcgt gcggctctgc cgcgagacgg cgtccatctt
     2821 gttggtcgcc tcctgcagct tcttctgcac cgaccccagc agctcgccga agcggccgaa
     2881 atgggtcttg acggccgcca gcacggtcca cacctcgctc gaccggcgtt cgatggccag
     2941 cgtgcggaac cccatctgca ggctgttgag gatcgcccag agcgtggtcg ggccggccac
     3001 cagcacgcgg tggtcgcgct gcaagatctc ggcgaggccg ggccggcgca gcacctcggc
     3061 gtacagcccc tcggtcggga ggaacaggat cccgaagtcg gtcgtgttcg gcgggtcgag
     3121 gtacttgtcg tggatctcgc gggcgcagac cttgacgcgg ttctcgagct gccgggcggc
     3181 cccctccacc gcctcggcat cgccacgctc ggcggcgtcg atcaggcgct ggtagtcctc
     3241 ctgcgggaac ttcgcgtcga tcgggagcca gacgggcgcc cggtcgggat ccttcccggg
     3301 cagccggacg gcgaactcga cccgctcgcc gctgtgctcg cgcgtctcga cattggtggc
     3361 gaactgtgac ggcgagagcg cctgctcgag cagcgtgccc agctgcacct ccccccaggt
     3421 gccgcgcgtc ttcacgttgg tgagcacccg cttcaagtcg ccgacgccgg aggccagcgc
     3481 ctgcatctcg ccgagaccgc gctgcacctg ctcgaggcgc tcgctcacgg ccctgaacga
     3541 ctcgccgagg cgggcctcga gcgtcccctg caggcgctcg tccaccgtct cgcgcatctt
     3601 ctcgagctgt gcggcgttgt cgtcctggat cgcgcgcagc tgatcggcga ctgtgccccg
     3661 cagcgcctcg agccgccgct cggtgccctc gccgagggcg gtcagctgct gcgcgaacgc
     3721 cgccagctgc tccccctgcc cccgcgccat catcgactgc ccctggcgca gcgactcgcc
     3781 cagccggtcg agtgcccccg cgacctcctc gcgcaacgcc cgcgcctgcg cggccgcgtc
     3841 ggatcgccca cgcgtcatct cctcgcgaac cgatcgctcc atccgctcgt ggtccgccgc
     3901 cagcccttcg acgcgggaag caatcgcggc cagctcggcc gggccccggc gcagcagcaa
     3961 caccacggtg agggccagga ggatgaacag caggacgacg gcaacgacga gaagggtttc
     4021 aatcatgtgg cggcgacatg gtgacagacc cgccttcgcg cggcaagctg cggctgtagt
     4081 acgattttcc aatgcccacc agagcctgcc ttgccgtgct cgtcctgctc ctggctgcgc
     4141 cgcgcgccgg cgccgacaac aagatcctgc acctggcgat tggcgatccg gccaggcgcg
     4201 accgcgaagc gccgctggtg ctcgacgcgg tcaccgacac cgcgaccggc gaggtcgtga
     4261 cgcccgagga ccttcccccg cggctcgcga agaccggcct gctgctcctc ggcgaagagc
     4321 acaccaccat ggagacccac caggtgcagc tgcgcatcct tcgggcgctc gccgactctg
     4381 gccggcgcgt actgatcggc ctggagatgt tcccctacac cgcccaggcc gccctcgacg
     4441 agtggcacgc cggaagcctc ccggaggagg aattcgtccg caagtctcgc tggtacgagc
     4501 actggggcta caactggcgc tactaccgtg acatccttct cttcgcccgc gaccggaaga
     4561 tcccgatgtt cgccgtgaac acgccccgcg aggtcgtcgc ttcggtgcgg aagaaggggc
     4621 tcgccaacct cagccccgag gaggctgccc acatcccgga ccggatcgat gccgacaacg
     4681 cggaccacct gctctacttc aagaccacct tcgagggcga ggaaggcccc gtacacggcg
     4741 cggggatgag cgacgagatg tggaagaaca tgctgagtgc ccaggccacc tgggacgcga
     4801 ccatgggata caacgccgtc caggcatgga agcgggccaa cgacctgggc gcgatcatgg
     4861 tggtgctcgt cggcctcggg cacgtcgcct acggcctggg aatcgagcgg caggcccgga
     4921 actggttcga cgggcagatc gcgtcggtca ttcccgtcac cgtgaacggg cccgacggcg
     4981 cggtcgcctc ggtacgcgcc tcctacgcca acttcctctg ggggctgccg cacgcgatcg
     5041 actcgctgta cccgtcgctc ggcctctcca ccgccgtcgg ccagggcgaa accgcccggc
     5101 gcgtgatcct cgtgcagaag gactcggtgg ccgagcgggc cggcgtcaaa ccgggggatg
     5161 tcatcgtgtc ggtggacggg acgccgctct ccgacaagga ggcctacaac cggctgatgg
     5221 ccgagaagcg gtggggcgac cgcgcccgca tcgtcgtgcg ccggggcggc gaggacgtca
     5281 cgctcacggc gctcttccgg aggacggtga agtgacgtcg ccgccagccg ctagacggcg
     5341 tacctcctct ccaccatctc cctgatccgc gcgcggccct cgggagccgg caggccggcc
     5401 ccctcggcct cctcggcctc ccgcgccagc tcctcgtcct tgatcacgag gcggaaccac
     5461 cgcgagaagt cgccccgcct gaagtggtgc agccaggtct cctcgtccac cccctcggca
     5521 atcttgacga agctgcgcag gttctcggcg cggagattca gcttgccttc cggcccgcgg
     5581 aaggcgaagt gctcgtcctc gatgagctcc ccctcggcgt acttccggcg gtggcgccgg
     5641 tgcgtgttgc ggggcggctc gaccgtgaag gtccgcgcgc gggacgaccc tcgctcccac
     5701 atggcaacat ccccgggctc cagccgcggc acatccggcg cagccccctc gtcccccacc
     5761 gcggaggcga agtcggccag ggccacgccc gcggcctcgc cgagcacgag cagcgtgtca
     5821 atcgactgca gcacctcgcg ggcgaccgag gacggctcga gggtgatcag cagcgcgcct
     5881 cgcaggcgct gcggcacgat ggatcccgac gaccggcggt cgcgcggcag caggtggtgc
     5941 gcctcgtcga ggacgatcca gtgcggccgg ccggtcgccg agcgaagctc ctgcagccgc
     6001 ggcagcagcg cctcgaagaa ccggggccgc tcgtcgcgcg ccacgtccgt caggttgacg
     6061 acgacgttct gtgccggcgt gtcgagaacc gtcatcacct cgcccggctc gggggtcttg
     6121 cgcgaggtcc ccagcacgac cccgcgctcg aacgtctcgt agtcgccttc ggggtcgacc
     6181 aggcagaact ggtagccgcg ttccgcaagg cgctcgagga atgcgctggc gagcgtggac
     6241 ttcccgcccc ccgaggcgcc ggccagcagc gccgagcggc cgtagggggg aaggcacacc
     6301 tcggagccgc tgccgtcgcg ccccaggagg atctcgtggc gccggagcga ggcggccagg
     6361 tcatcgaggt cgttggccag cagtcgctcg acgagctcga cgacaccggc gccgtgatcg
     6421 gcggaagtga ccaggtcggc ccgctccttc agcatcggca gggcgttggc gaccgccgcc
     6481 ccgcactcgc acgactggag cagcgcgtgg tcgttctccg cgtctcccac cgccaccacg
     6541 ttgtgagggg acagcgacat ctccgccagc accgcgagca gcccgctcgc cttgttgatc
     6601 cccggcggca gcaccatcac cgcgtccttg ttgaagatca cctgcagctc gaggccgaga
     6661 tcgcggatcg ccccgagcac cgccaactcg ttcgggcgcc aggtggcgac gatcacctcg
     6721 cccgtgtcga acggggtgac gccgcgctcg cgcagcgcct cggcgaacgc cggcggcggc
     6781 ggttcggcca gccgcctggt ctcgcgtcgc gccgggtcgc aggacacgcc gccgttctcg
     6841 gcgacgatcc ggtcgaacag ctggtactcc gggaagaccg actgcaggtc gtcgagccgc
     6901 cttcccgtca ccaggatcag ccgccggccg gaatcgcgca cgcggcggag ggcggcaagc
     6961 gtcggctcgt ccacgcggcc gtgccgggcc agggtgccgt cgtagtcgca ggccagggcg
     7021 acgtagcgca tggagtgaga cccggccgtc gaaacagcaa gagcagggcc agctgccttc
     7081 aagtctccca cccacgcgcc gccgccgcct ccgtgtagaa tgccgggaac agggagccac
     7141 cagcccaact atgatcgaac gcttccgaaa cccgtcgccc aggatccggc gggccctgga
     7201 catcgcgggc atcgcccacg acggcgcgtt ccgcaagggc accgacgtcc cgtacatcca
     7261 ccacccggtc gccgtcgcgg tgattctcga ggcctacggc tatcccgagc cgctcgtcgc
     7321 ggcgggcctc ctgcacgaca cggtggagga caccaggtgg ggaaacgccg aggttcagcg
     7381 acggctcaac caggtcaccg aggggaggct accctccccc gccgagccga tggcgttccg
     7441 ctcggcgttc tgccagtacc tgcgcgacga gttcgggcgc gaggtattcg gcctggtcgc
     7501 cgccgtgacc gagaagaaga acgacggcgg cgtgccggcc gactggctcg agcggaagaa
     7561 gaaccagctc gacaagctgg ccaacgccgg accggacgag gcggcgctca aggccgccga
     7621 cgcggtacac aacatcgaga gcaccgcgcg cgacatcgag gcgctcggcc tgggcgtgct
     7681 cgaccgcttc cggggcgggc cgctcgtcgt ctggcactac agcgcgttgg catcactcgc
     7741 ttcgcgccgc atgccggccg gcgcgccgct ggccgagcgc gtcgtccggg cggccttcga
     7801 cctcgaaagc gccgtccgcc gccggcggcc ggttcgcgac ggctcggcgc cctacccgga
     7861 accggtggtg atctagacgg ctcagggctc aagcatcagc tccttggggt gaggttcgag
     7921 gtacacctgc cctgcgaggt actcgatccc gcagcgccgc accaggtgcc gcacgagcgt
     7981 gatcggcacg atgagcggga cgaggccctg ctggtagtcg gcgatggcct gggccagctc
     8041 ctgccgcgac gcctcgtcca ctgccttcct gaagtagccg gccatgtgct gcagcacgtt
     8101 gacgtggcgg ctcggcgtcg ccacgacggc aatggcgcgc atgtagccgg tctcgtaggc
     8161 cgcgcgcagc tcggcgcgcg gcatgtcgcc ggcgcgcgcg accaggcgcc cgagcgcggc
     8221 gtaccccgac ggggagtgcg cgagcagcga cagcttctgc gccgcgtgga aggcgaccag
     8281 cgagcccgcc gtccatcgcc ccgagaagaa ggcgcgcagc cggtggtgcg cgaacacccg
     8341 ctcgacgaag tgctcgcgta gccgcgggtc ggcgagctgc ccctcctcct ccaccggcag
     8401 caggggcagc cgcgagatga gggcatcggc gaacaggccg cggccctcac gcgacggcat
     8461 gccgcccagc gtgtagacct tcacgtgctc catgccgcag ctgggcgaat ccttcttgag
     8521 gatgtagccg tcgagcgcct cgccggcgag cgactcgaca cgcgcggcag cccaccggcg
     8581 catgcgatcg gtgtgatcgg tcgcgctctc cacgccaacc agccgcacct ggtccccgtc
     8641 gcgcacgagc cggatggctt cgcgcggcgt accgaggccc acctccacct cgggacacac
     8701 ctcgacccac tcgacgaact tcccgaaggt ctcgacgagg aaggcgtcgc gcttgtgccc
     8761 gccgtcgtag cggaccttcc gccccatcag gcacgacgac accccgatgc gcaccgtctc
     8821 gaacacagtg ggcccctcaa agacgatttt gaaccacaaa ggggtgaaca gggccggacg
     8881 ccggtcttcg gcacggcgag gtcgagcgcc gggttatcgg tggccctcgc cgcgcgctgc
     8941 agccagcaca cgacggccgc gccgtcgggg ttgggcagcc caccgcgccg caccgcaacc
     9001 cgcgggtcgt catcgccgcc gctgtccggg tagaatggtc cgcgccaact catgcccgcc
     9061 accgtttacg gagggcccgc acgatgatac gtccgcgcct ggccctggtg ctcggcttgt
     9121 ggctgaccgc cgctccggcg ccgtctgccg ccgtgccagg acaggccggc cccgcggccg
     9181 agcccgtggc gatcccgcgc tttgagcgcg gtgccaaccc catcgcgctc ggcggccccg
     9241 cccgccccgg gcgctacatg gaagcctcgg gccttcgcgc cgccttcctc gggcgcgaag
     9301 acgggtcgtt cgaggcgtgg gcctatcccc tcaagctgct gcacgacttc aacctgtcgt
     9361 tcggcatcgc cgcctacgcc gacccgatcc ccggcgccgc gctcgcctcc accgtggaca
     9421 tccggcccga gtcggccacc gtccgctacg cccacgcagc cttcaccgtg gacgcgatct
     9481 ggctcgtgcc gcgcgacgag ccggggggcc tcgtcctgct cgacgttcgc acctcggagc
     9541 cggtcaccgt ggtcgtccgc ttcaggcccg acctcaagcc gatgtggccg gcggccctcg
     9601 ggggccagta cagctactgg gacgacacgt tgaaggcgtt cgtcgtcggc gagggcagcc
     9661 gcaagaacgc ggcgctgatt gggtcgccgt tcggactgac gccccccgag cagccggcgc
     9721 acaacctgcc cgacgcgccg tcgcaattca cgatccgggt cacccccgag caggcgcgga
     9781 aggggctcat ccccatcgcg atggcgggca gcgtcgagaa gctcgacgca gccaaacaga
     9841 cctaccaggc cctgctggcc tcggccgagc ggctctaccg cgagcaggag gagcattacc
     9901 gccgcctgcg cgaggagcgc accagcgtcg agacgccgga cccccgcatc ggcctcgcct
     9961 tcgagtgggc gaaggtcgcg ctcgacaagg ggttcgtctg caacccgcac ctgggctgcg
    10021 gcctcatcgc cggcctgggc ccctcgggaa cgaccgagcg ccccgggttc gggtggttct
    10081 tcggcggcga caccttcatc aacaactggg cgatctccgc ctacggcgat ctcgacacgg
    10141 tgcgccggtc gctcgatttc ctgcgcgagc gccagcgggc cgacggcaag atgatgcacg
    10201 agctgtcgca gggggccggc tacatccgct ggttccagga ttacccctac ggctactacc
    10261 acgccgacac gacgccgctc tacatcatct cggtgcgcga ctacgtgcgg gcgaccggcg
    10321 acgccgcctt cgcgagggac tactggccct cgatgcgcag ggcgtgggac tactgcgtct
    10381 cgaccgacga ggacggcgac ggcctgatgg acaacacgaa ggccggcctg gtcatcgaaa
    10441 ccggggcgct gcgcagcgcc gacgtgcaga ccgacgtctt cctggccacg gtgtggaccg
    10501 aggcggcggc cgcgatgatc gacctggcgc ggctggccga accgggcttc gtgtccaccg
    10561 cggaggcggc ggccgacaag gcgcgcgccg cgatcaaccg ccggttcctc gatggggcga
    10621 ggcggcgcat caacttcgcg atcctcaaga gcggcgccgg gcaggcggag gaaaccgact
    10681 ggccggcctt cggcatctgg cgcggcgtgt tcgagccggg tcacccggca atcgccggga
    10741 tgctcgacga gctggcgcgc tcgggcctcg ccaccgactg gggtgcgcgg atgctgtcgc
    10801 gcgagagcgc gctgtacgag ccgctctcgt acaacaacgg cgcgtcgtgg ccgttcctct
    10861 cggggtgggc cgcgctggcc ctctacaccg gcgggcggcc ggaagccggc tggcagtacg
    10921 tcgacgcgct ggccgacctc accttcctcg aggcgcgagg cttcatcccc gagctgctct
    10981 cgggcgaccg cctgcgctcg atcgacgcgg ccgtgccgca ccagctgttc gcaaccaccg
    11041 gcttcgccac ggcgctgatg cgcggggtgg tgggcctcga ggaaaccgac cagggccttc
    11101 ggctcacgcc gcggcttccc gccggctggc cgttcctgcg cgtgaagcgg ctgcgcttcc
    11161 gcgacggggt cggggacgtc gagatccgcc ggcggccgga cggctacgcg atctcggtct
    11221 cgaacctccg aaagccgctg gcggtggggg tccgaatcgc gctgccaccc ggcagcgagg
    11281 tggaaggcgg cgggcaggcc gagatgccgc tcgccaccgt cccggccccg aaaacggtta
    11341 cgctccgcgt gcgcccgggt gtcgagctgg cgcccgtgca gcggccgctc gcgctcggcg
    11401 acgagtcgca ccgcctgcgg gtgatcgaga cgtcggtggc cgccggggtc ttccgcgcca
    11461 ggctccaggg gctgcgcgga cacacctacc gcctgcgcct cgacgtgcca ttcgaggtgc
    11521 tcgcgatcga gggcgcctcg gaagtgggcc gggatggcgc gtcgcgcctg gtcgaggtga
    11581 caatccccga cggtccgggc gagtgggtcg agacgacgct gaccgtcggg cttggcagac
    11641 gccggtaatg gggtaacagg ccgccggagt ccatcctggc tttgggagaa cgacgtatga
    11701 gacgagtgag acgattcgca ggggcatgtc tgaccatcgg ctgcctggcc gcggcgagcg
    11761 cggcagcgtg tcccccgcag cccagacccg ccgcggcgcc tgtgcagaag gcgccgttcg
    11821 gcaccacgaa ggaaggggtg gccgtggatc tctatacgct gacgaaccac cacgggatgg
    11881 ttgcgaaggt gatcacgtac ggcgcgacgc tgaccgagct gcacgtcggg gacgcttccg
    11941 gcaagccggt caacatcgtg ctcggctacg accgcctcga gccgtacctg gcgggggtcc
    12001 cgtacttcgg cgcgacggtc gggcgcgtgg gcaaccgcat cgcccgtgcc gagttcaccc
    12061 tcgatggcaa ggcgtacaag ctcgccgcca acgatggccc gaaccacctg cacggcggcc
    12121 tcaagggctt cgacaaggtg gtgtggcggg ccgaggaggt gaagtcgccg gccggcccgg
    12181 cggtgaagtt cacctaccgc agccccgacg gcgaggaggg ctaccccggc acgctcgacg
    12241 tgagcgtcac ctacaccctg accgacgcga acgagctgcg gctcgactac cgcgcggtca
    12301 ccgacaagcc cacgccggtc aacctcacca accacagcta cttcaacctg gcgggcgacg
    12361 gctctggcga catcctcggg cacgtcgtga ccatcgcggc cgacgggatg acgaaggtga
    12421 acgagcgcct gatccccacc ggcgagatcg tcccggtgaa gggaacgccc tgggacttca
    12481 acgtgccgac cgccatcggc gcgcggatca agcaagtgcc gggcggaccg cccgtcgggt
    12541 acgatcacaa ctacgtgttg cggaaggcag atcccggcgg cctgcccgag cttgccgccc
    12601 gcgtcgtcga gccgaagagc ggccggacga tggaggtgct gacgaccgag ccggggatgc
    12661 agttctacac gggcaacttc ctcgacggat cgctgaagaa ccggatcggg gtgccgtaca
    12721 agcagtactc cgccttctgc atggagacgc agcacttccc cgacgcggtg caccacgccg
    12781 ctttcccgtc cacgatcctg cggccgggga gcgagttcac gagcacgacg atctaccggt
    12841 tcgggcacga gtaagagagt caggggaagc ggatggcggc gaactggctc gggctccggt
    12901 gggcgtcagg cgctcgcccg caaggcaacg cgcgccacgc gcgcctggtg gagcgccgcc
    12961 tctcgacggc ccgggtggtg ctggccgtgg cgtcggtcgc cgccctggtg gtcgagcacc
    13021 gcgcgaggct cgacccgctc ggggcgagcg tcctggccgc gttcgcgctc tacagcgcgg
    13081 tgctggcgct cgaactgcgg cgcgagtccg acgacgccgc cccgccggtg ttgggcctgc
    13141 acgcggccga cttcgcgtgg gtgctggccg tcttcagcac caacgccggc gtgaatgggc
    13201 ctttcctcgg gtactttctc ttcgtcctgc tcgccgccgg gtcccgcttc ggcctcctcg
    13261 agacggcgct gaccggcgcc gcggcgaccg ccggcacggt ggccgatgcc gccgcgcgga
    13321 cctggctcct gccggccgtc gccgtggact ggcacatcgt ggccatcgac ctggtgtacc
    13381 tggtggtcgg cacctggctc gtcgcctacc tggccgagta cgagcggcag caccggcagc
    13441 aaacctgggc gaccgccgag atcctggggc ggattcgccc tcaccagggc ctggtcgcca
    13501 gcgtgcagaa cgtgctggac ggcctcctcc ggcagctgtc ggcgcggcgg gccatgctcg
    13561 tcatcgccga tctggacagc gaccgggtcc tgctctggga ggcgcgggcc gacgagtctg
    13621 ggggccgcgc cacgattcgg ctccgccagg agatgcgagc gagctgtggc acctggctct
    13681 tcgaggtgcc ggctcgagcc gatgcctggc ggggcgttcg ccggcgccac gccagtcccg
    13741 gcgcggtcgt tccggcgatc gattctcgcg gcgaccggtt cgccgcccca ctgagcctgg
    13801 cgccgctgct cgacgccccg ttcgagtggg acgaagtgct gtgtctcacc acgctggttg
    13861 gcgagggctg gcaggggcgc ctcttccttt tcgacccccg gatctggcgg cccggcgagc
    13921 agaacctcgt cttcctgcag gtcgtgatgg ggcaggtggg cccggcgctg ttcaacctgt
    13981 acttgcagcg gcggctgcgg tcgcggtcgg gcgcgctcga gcgcgcggac atctcccgcc
    14041 agctgcacga cggggttatc cagtcgctca tcggcatcga gatgcagatc gacgtgcttc
    14101 ggcgccaggc aggcgcggac gccggcatgg cggccgacct caacggaatc cagcgggtcc
    14161 tggtcgacga gatcctgaac ttgcgcgacc tgatgcaggt gctcaagcct gccgacgtgg
    14221 ggcccgcgca gctggccgag cacctggccg gggaagtcgc gcacttccgg agccggacgc
    14281 acatcgatgc ccgcctctcc tgcacggagg aggagataga cctcagtccc cgcgcctgcc
    14341 gtgagatcgc ggccatcgtt caagaggcgc tggccaacgt ccgcaagcac tcgggcgcga
    14401 ccgcggtgat ggttcgcctg agccggcacg acgccggctg gctgctgacg gtggacgaca
    14461 acggccacgg gttcgcgttc gacggtgcga tggacggcgc cgagctggag gcgaggcacc
    14521 tcgcgccggt gatcatcggc gagcgcgtgc gggcgatcgg cggccggctg accgtctcgt
    14581 cgcagcgggg cacaggctcg cgcctggaca tcagcttccc tggaagacac catgcctgat
    14641 cgcgagcggc gaacccggat cgtcatcgcc gacgaccacc cgatcttccg cgaggggctt
    14701 cgccgcctgc tcgaggcgga gccccgcttc caggtggccg ggcaggcgca caacggcctc
    14761 gaggcggtgc ggctggtgga ggagctccgg ccggacgtcc tcctgctcga cctggccatg
    14821 ccgggcgccg gggggatcca ggcgctccag gagatcgcgg accgctgcct ggacgtcaag
    14881 gtgatcctgc tcacggcggc catcgacacc gccgacagcg tgcgggtgct gcagctgggg
    14941 gcgcgcgggg tgatcatgaa ggagtgcgcc acgcacctgc tctacaagtg cctggacacg
    15001 gtgacctcgg gcggatactg ggtggggcac gatcgggtca acgacatcgt gcagcacctg
    15061 cgggccgggg ccacccgccc gccggcgcca gccgcgatgc tcaccaggcg cgagctgcag
    15121 atcgtcacgg ccatcctgaa cggcgccacc aacaaggata tcggccagca gttcaacctc
    15181 agcgagcaga ccgtcaagaa ccacctctcg cacatcttcg acaaggtggg cgtgtcgaac
    15241 cgcctcgagc tggcgctcta cgccctgcac cacaagctgg tcgagggagc gcgcaagtag
    15301 gacggggcgt cctcgcccgt gcgaagaagg ccggttcgca cagtacttag gtcccggccc
    15361 gccttggtac ctcggttcct gcgccccagt accagcagcc cattgtgcct tgaatccagc
    15421 ggctgaatag tctgctgtgg gatccagcct ggctgaaaga tgaccctggc ggcaggttgc
    15481 cgcgcaactt ttctgcagca ccagattctg ccgatcggcc acagcgcagg tcgaaccccg
    15541 acgttcacgc ggatgacgca ggcgcgcggt caagtgagga ggtttcagat gaagaggcgc
    15601 agacgacggg gaggcactcg gactacacgc gcaggttggt cgcgtcgcga cttcctcagg
    15661 attggcacgg ccgcgggcgg cggtctgtac ttcgcgacga agattggagg cgtcccgtac
    15721 ttcgtgcggg cggcgatccc gggcgggagc ctggatcccg ggttgatatc gaagtaccag
    15781 acgccgctgc tgatcccgcc ggtcatgccg cgggccggca gcatccagat gcggggcgga
    15841 aagacgggcg actactacga gatctcggtg aggcagttcc agcagcagat ccttcccgct
    15901 ggccttccgg caacgaccgt gtggggctac ggcccggtga agtccgagag caagaagggc
    15961 ctgctcctgc acaacgcgcc ctcgctgacc atcgaggcca gccacaaccg gcccgtccgt
    16021 gtgaagtgga tcaacgagct gacggacggc aacggcaact acctgcccca cctgttgccg
    16081 gttgacccga cgctgcattg ggcgaacccg ccggggggcc cgacgggacg ggacacgcgg
    16141 cccgacttca gcggcaaggc ctacgtgcga ccgaacgagt tcaacgatcc cgagactcag
    16201 tacacctcct accagggacc cgttcccctc gtcacgcacc tgcacggagc cgtcggcgtg
    16261 ggagacgaga gcgacggcta cgccgaggcg tggttcctcc cggctgcgaa gaacatcccg
    16321 gcggggcacg caatcgaggg cacgtggtac gacttcttca ggaggaaggc cgcagcgtac
    16381 ggcgccacct ggggaccggg attcgcgacg ttccagtacc cgaacgagaa ccgggcgtcc
    16441 accatctggt accacgatca cgcgctcggc atgacgcggc tcaacgtcta cgcggggccc
    16501 gccggtttct acatcgtgcg gggcggcccg gccggtgaca aggccgtcct cgactcccgg
    16561 acggggctgt ccgccacgct gcccgggcct gctccccagg aaggggacaa gttccccgcg
    16621 aacaagcctt acagggagat cccgatcgcg atccaggacc gggcattcaa cagtgacggc
    16681 tcgctgttct acccggacac gcgcgcgttc tttgacgagt acgacgggcc atccataccg
    16741 gacaccgaca tctcgcccat ctggaacccc gagttcttcg gcaactgcat gatggtcaac
    16801 ggcaacacgt ggccgttcct caacgtggag cagcggcgct accggttccg cttcctgaac
    16861 ggctgccagt cgcgcttcct catcctcgac ttcgccagca tccctggcgt cgaggtctgg
    16921 cagatcggca acgagggagg gttcctcgga gcaccggtca acgtcacggc cgacggcaac
    16981 aaccgcctgc tgatggcgct cgccgaacgc gctgacgtga tcgtggactt caccaacgtt
    17041 tcccccggga actacgttct cggcaacctg ggccccgacg agccgttcgg cggcggcgag
    17101 cccggcattg acttcgacgc tgccgaccca agcagcacgg gccaggtgat ggaattccgc
    17161 gtggggccgg cgctgacggt ggataagaca acgcccgcgc cgttcctcgt gctgcccccc
    17221 gtcacgccgc tgccggccga ggtgcgcacg cggccgctcg cgctgatcga gatgatgtcg
    17281 atgcactacg atggtccggc ggaggcgatg ctcgggaccg tcggctccga cggcatgccg
    17341 gcgcacagca tgtggatgga ccccgtcacc gagaaccccg ccgtcggcga cacggaggtc
    17401 tgggagctgt acaacttcac ggccgacgcc cacccgatgc acgtccacga ggtcgtcttc
    17461 gaggtggtga accggcaggc gctcgagacg aatgcggacg gcgagaccgc actgccggtc
    17521 agcctcgttg gcgaaccccg gccgccggag ccctgggaaa ccggcttcaa ggacacggtg
    17581 atggcctacc cgggggaggt cacgcggata cgggcgacct tctccaaggg gggccggttc
    17641 gtctggcact gccacatcgt cgagcacgaa gacaacgaga tgatgcggcc ctaccggatc
    17701 ggtcccgtgc agcccggaga gccggggctt ccggaatgac cgcgccgcgc cggcgaggtg
    17761 ttgaggccgc gtccgcatag cctgaattcc gctgggtgct cgcccgccct ggtcgccgtg
    17821 accaggcggg cgagacccag ccccccgcct cgaactcccg ccatcttgtc cgatccccac
    17881 cgtgcgtgtt gcctgatcct cgattccgaa cctgacccag agccgcgccg tagtactttc
    17941 ctcctactcc gaaccggtac ctccgtcttc ctccgccagg tacttctgtc cacgctcatc
    18001 cggtaccgcc gtactccgac acttcgtacc ttcctgccat tgtccagatt cgcaatcgac
    18061 tgaaaagtag agatcgggcg atctcaccac gctgctgttc agagccgggg tctgccgccg
    18121 gggagcatcg atggagcagt tgctggagcg gccacgagca gcgcgatacg aaatgtctgc
    18181 acccgtcatg tacaggcagg ttggcgagga ctcgtggcac gagggccgga cgctgaacgt
    18241 gagcagcagc ggagtgctgt tcacctgccc cgcgccggcc ctcgagcccg ggacgaaggt
    18301 cgagttcctc ctcgtcctgc ccaacccggg gctgcgcgag cagccccgcg tgcagtgccg
    18361 gggggcgatc gtgcggcacg agcgccggcg ggccgggccc gcgtgcgcgg tggccgcgac
    18421 gatcgacgcg tacgtcatcg tgccagcggg gcagccgtcg gcgggaaggg tggacgcgtg
    18481 aggggaacgg aatctcagag gagagccaag atgagcagaa gcagtctcag gatcgcgttc
    18541 gggctgatcc tggcggttgc cgtcgcgtgg ggcatgagcc ccgacccgcg agcggccgcc
    18601 ggggcggccg ccaacgcggg cgcgagcgcc gtcacgtggg gcgggacgat cgcctcgacc
    18661 gtggcgtcgg cggcgcttcc gggcacgccg gccggcgagc cggagccgat cccgtcgcgg
    18721 tcgctggccg gcgccatcac cgacgacgcg gcggccaacg acgcgccgca gggaaatggg
    18781 aaacagggca agggccaggc gaagcccaag ggcaagccga cccaggagga cttctacgcg
    18841 aagttccaga aggtgtcgcc cgccgaacag aaggccgccg ccaagcgctc tgccgagatg
    18901 ggcatgctgc cgggcgcggc cggcctcgag ctggcagccg cgtccatcac gccggcggat
    18961 ctcccggtcc tcgagggccc aggcggtgtc ccgcactact tcggtccgta tggcaactgg
    19021 gcctacagcc ccctgccctc gttcccgatc ctgtcggtga gcgtcgatgc gggtggctcg
    19081 ggctatagcg cgccggaagt cttcatcaag gacgtctacg gcacgggaag cgattccgct
    19141 gatgcgccgc tcatggggac ggccaccgtc acggacggcg tcatcacgga gatcgcaatc
    19201 ccggagggtg gcaagggatt ccacgttccg ttcgtggaga tcattgatcc gaccgggacg
    19261 ggcgccgagg ctacggcggt gctcaacctc gagcggccga gcggcggcat ccgcaagttc
    19321 gtggacaggc tgccgggact tgggcccacc ggagcgaaca acatcatcca ggcccacggt
    19381 cttggccagt acctccccgt cgccgccaag gaggagtggt gcagccggcc agaggcgatc
    19441 gc
//
LOCUS       DAOPQX010000025        19209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3483_length_19209_cov_5.034144, whole genome shotgun sequence.
ACCESSION   DAOPQX010000025 DAOPQX010000000
VERSION     DAOPQX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 19209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19209
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3483_length_19209_cov_5.034144"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKK95_02550"
     CDS             <1..664
                     /locus_tag="PKK95_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17115.1"
                     /translation="ILVGLACPRGVEPEVWDWLTRLLPARLGHTLQIARVDGEAEIAW
                     ANNQLVQKLLRGDWTHLWFVAADMRPYPQTHTILEATADVAIGVSFVVEPDPLVPQFT
                     HLWPNVSRSDDGLRFPPLKWDELADGPLDVDGGGMHCTLVRREVFESPRVRTDGGAWF
                     RTRRDAFDRRMLTDDADFCWRAKQAGFSVRAYPGALSGHVKTCDVGGVWTAARVRALS
                     EV"
     gene            667..1077
                     /locus_tag="PKK95_02555"
     CDS             667..1077
                     /locus_tag="PKK95_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17116.1"
                     /translation="MSIATTIQLLDSAALTADGTTDYFKIPTATMGELHVEVVSGSGT
                     ISGFDLWAQSQSEAGASPVDILWDRAVKTADAATEGTARSNVRDVVDGKITTTAEYFW
                     AKIDALAASRFRLKWKLSGTSTPTLTLNAWLVVK"
     gene            1077..1775
                     /locus_tag="PKK95_02560"
     CDS             1077..1775
                     /locus_tag="PKK95_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17117.1"
                     /translation="MAARPITTAAVVLAKNPGIDSAQINTTELSRCIDAASALLEKWC
                     NRIFVEATYAALHSGERALRIRDDEGRAGTRLYLADPVTRLLTMPVSAVSSCTEDGTA
                     ITTFRLSTAPTFTDGDYGALVSDARGIVTRVSIAGGEITPVPWAPGVANIKVAYTAGY
                     KTSGSTEPLIPADLVEACCHLTWLLFNEGPRSGAEIMSEAGTFANYSRLLVPSVADAI
                     KRYALPMSPMTLEA"
     gene            1776..2393
                     /locus_tag="PKK95_02565"
     CDS             1776..2393
                     /locus_tag="PKK95_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17118.1"
                     /translation="MQVFATLDDLDALLRMPQLSVKEIRKCALRGNDVAIGLAIDELR
                     SELNREEPRYFRRRARGLLKDFRRMARLYRATFSRGLGSALAARAELVAVGLGGSRMR
                     AAAPLEVGGKYQQFVEAHTRRVKGRDVRERVATASGRRRMTTIAQGYTQVREHLRMRP
                     EDAHRYLLRAVQAKLSVMIVPTSRALAGLIATGKVPTAKTLRAGL"
     gene            2390..3367
                     /locus_tag="PKK95_02570"
     CDS             2390..3367
                     /locus_tag="PKK95_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17119.1"
                     /translation="MNLLVNPGFEAGDTGWTLATGATVTSAYGAHMGVNACRLRWKLG
                     TIIPPVPSIRGAASQEIASTAGLVYELSAWVALESGQAGARLQVQNPAGAAWEDVDTI
                     LYTDLTDDWVRLSGSVTAAGDTLAVRVVVYEGAQSKGYILADDLIAGVPVEIQLMPRG
                     LRKAYVDFYNKIASISTADGYYHTVSTIIPRMAWPNEQNAPRVFPYVCVPADHQLEAG
                     DYPEVDSGSVRVRLRQPVIVVLKRSWSDDAETGAAADALNWHDDILRAVMPAAQSGAW
                     NAGSVIIRDITPHKFRVLADPVEGGAPFVSVEFLIDLIVSRDDLGPEAS"
     gene            3367..3597
                     /locus_tag="PKK95_02575"
     CDS             3367..3597
                     /locus_tag="PKK95_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17120.1"
                     /translation="MAKWLRFTLPKGMHTWAVRRDDPRVLISGHIVDGAQLPYDDVHD
                     ADGYVLRGQAEIVEVGPKVAEPKPETGPAASD"
     gene            3629..4174
                     /locus_tag="PKK95_02580"
     CDS             3629..4174
                     /locus_tag="PKK95_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17121.1"
                     /translation="MALELKNIAIGGTTLSLGAYVTAGGAGSLTDVGLLKDGSELNIA
                     KEIYEVKSSSANGVLRAIPIDSTYELKAILQEATLDNMRKALGQPSANLTGTAPNKTL
                     LVGDMAEEYFQGTVSTGGTGGTPATRLMTFWKLYVKAVETLAFKKGQEQMYGITFGMV
                     ADQSVATADKFYKVVDSGGVA"
     gene            4171..4749
                     /locus_tag="PKK95_02585"
     CDS             4171..4749
                     /locus_tag="PKK95_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17122.1"
                     /translation="MIGDFDSGRRVVLAGGRRYLCRPPTVRTVDRVLTLFLTEVSALH
                     AAAADVSLTPEEILRVLSGDRRLAEVLDTCVELWGAAPGELVELLADNRDLQVELALA
                     VVGLCDAAWIWSTLHLAEALEAQEQAPIGPEDATTQDAVLCRIAAAFGRPPHELLDWP
                     YGAFVRAVQLGYGSDPLAAHPAMTTTLADLEV"
     gene            4753..6933
                     /locus_tag="PKK95_02590"
     CDS             4753..6933
                     /locus_tag="PKK95_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17123.1"
                     /translation="MAGNELNVRLRIESILKEKGLLDLKQRLEAAEKEVVKLNATAAA
                     SSGAFRAMGTAIKSALLPLAGFFAVGKVVSFLYQAAEGQREMERRTMALSRQIQQLGV
                     DVPQAMERVEEFTTGLANLTGVVDDESIPALTQLIGLTGSVEGGIAGVKLAADIAASG
                     VRDFAGAVELVASILAGRANEAARQLGINIKDVNGHAKSNVELVSEIIEKQEALAEQA
                     AKNTEQTSKLGAAWDLFGDTIGDYVRPALDVLKSVLAAVLKGVLMTIDGITIVAHRAI
                     QLPSRIWAGIKGDREKLKQLDQELLDVEIELAQKYIDAENKALETTRLSAEEKAALLN
                     AARLKDLEAEQKAAEERRKLAEKEAEERRKLAEKEAEERTENEREAELALLEAKIKIA
                     EEGSAERLALEMERLDKLMAAELAAANLTEQAKLAIVGKYALLKQDLLNKGYEDQAKR
                     DEKAAKEKRDFDEGIRKDLARLQLELAEDAADQRAAELAAAEQDYVDDYNRALKLGGD
                     IANVEEKYRRKRASINKKYDDAQEKVDKAAAANKVQMYATAAQMSIAALQTLFGNSKS
                     LAIAQAIIDTFLGANRALGELGPLGPPVAALIIAAGLKNVMIIKKTNPQKEGGGGAAV
                     PRMAGGGMVLGTSLVQVAETAAARPELIMPLRSRVTEELLAAALARAAQSPAAQSAGL
                     GAGQSTIINIPVTVNTAVPERRALRELHRQLQRVAREDLARRAG"
     gene            6930..9131
                     /locus_tag="PKK95_02595"
     CDS             6930..9131
                     /locus_tag="PKK95_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17124.1"
                     /translation="MSPRPAFRVLLTGPDGVEDDITTRVDVSSLGRLTEELEDDLLQL
                     VHSDISLELDDADGWASARLGAATRGEVWEVVIERETGRRRPLWTRIFGGALDVPGSI
                     VIDRKERVVELEVWSYSKLLELAAASALARSVTNHMADATAAALTATVSPDTSLLVAG
                     DQITLTGGGHSDSRVIAIVNSATEVTVTEAWSATFTSATLTLDTPYPRSAVAEDLARD
                     LYAAAGIADATIDVGAVLSGVLFPTAMSADGLAGGTPSSLVEFGGKLLLYLGGKRYEA
                     TSSSSGWTDAGADTVKLDWQPYLATEPAGLQPGTAAKIYDYVDAAHPYYELKVDKSGG
                     SDHLRIYKDGAPLVTVDSVGISTPTPDGEFTFYTYEVAGYWGEVWVSYRATTKTRSRE
                     WDADLGEFVWVDHVLYKNRTVRYATDGTLLQTIQAAGPLRFVRADDRMAWLVTHLGGD
                     QDDLQALAEPAVHLWYHGASQGVLAAASVDMWTFRKIGTAYYAGIQGGDVVLWDAVTR
                     DLLAIYPLVATPGGGALACVFTAGGTIDAEYDGWTSGMYFSLALAATNVVPYADFADM
                     SCAAALRELAVVTMSRFFVDQYRIGYLIGRAADWITGRAAIEPGVPLEQQVRPIWDSL
                     CRSVAVTGEAEDGTEIEVLVGDAGDSQCRLEVSTQLPMTSGLAGVLAAAYLSYLSVPR
                     QQDEETIDEPETGPVRVLDRVVRDGRTWVTLRVESDLRDREQELLLVEEVA"
     gene            9128..9589
                     /locus_tag="PKK95_02600"
     CDS             9128..9589
                     /locus_tag="PKK95_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17125.1"
                     /translation="MSGYAWWPMVRITPANGPEVVRDLKSDLSTLWGIGPTKIAVKHA
                     PQIKQRQDVNDRERPLLKGFRVEVDLTFEVSDEMTDHDILAEIVTACLDQYQVVELSM
                     DGGLTYRGVVLTDYLGPDPLGGKPVAGAKYELHFRCRELVTELPAIGTGNW"
     gene            9702..13118
                     /locus_tag="PKK95_02605"
     CDS             9702..13118
                     /locus_tag="PKK95_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17126.1"
                     /translation="MRRWLLLILALAVTPAFSAVQTTTVEGYVLNPSGGAHTGTIVCT
                     LSHAGASDDLTTGESERVASKYSTTIASSGAVNFALVPNDAITPANTSYTCVFTVGSG
                     ATRSSWTERWSVGTSPDPVDIGDITRLDLADGLPVTLDALTCTDCVTLGTETIGNYAG
                     SPSEGGGALSLQGADAATGVPVDADGDAVNEARIRAGKIYGETVNRTVPCWDLGGGAE
                     DSPDGTCDVFFDSYRGAVRGDGTPLFMGPVSIFANYGDFHPLGVGSKYFDRLDWELTQ
                     CKAYGMPCGVNVQTASHYNPPIRLPDANIKEIYDAGVSVGSHTDSHCAMTLLNQSFSP
                     NYAVLENSGGRYTNGCGTSSPTCVHTGTVSVTEGSANLVGTATNFAGECNDNTDDFAP
                     PGGRIFIDTDGDGTVEFNVRECYGTQTACNKDADCGGPKCVADFSSDILTVTDATHLV
                     LRDALPTDTDRDGTTGETFATAIFEIVSGPGQWRQDLKTSCAVMENAIGDPGYKCRWF
                     GAPGGWIGSEIYETAYAASDIDAGVGSGSGWDNQEFEVAGAPTTATVLERFHINCGAS
                     ADSIKAFMETAAKSRLNLFFSGHTPIAIDDDECRGPDHVLGNYDDASDAWSQANFPAY
                     LQKCRELMRDGRGICLDPYHAGVLMRAWSGNQLGVNLIGNSDLADFNSGWNVGSGNVT
                     KRFPWEDPKGTININDAPGSTDQLDYDATNQWAVFKYENADGDGVADDVSISQRVKLS
                     SGYYQIGMWYKLLQDITGANNVAGVRLCIGSTWWAFGGKQGVSDLTPQWVEHYKGADN
                     SATGTTYFNQKGCTNFQVGNAGTEDQIIGYFYVPPEQDGIVQDIYVEFRDCDGDGTSN
                     DCGNDPYIAFRDVTLVKYVPQPTDSRYGGMGVTPLLSVTKVPHNSARTDSGAWTWLWT
                     PNDQNSGQSANPTTDFVTARTIYPAVGPYTVFAIGKYNINSDDTWRGNASPMMRLGNT
                     TIGGATGTAAGYGLGQTIYKGSNFSGAWVGRSTTSKGVDWLLNLPATSGATEPAIRYK
                     ALNYTKVDTDAIASWGVGGSHKAGDNTQDEVAQLWGAGTLSLEGGLKLGTDAEEAVAT
                     GTNSLCIKRKTDCGASECVFIDLDCNGTVDAPDFCMHSDGIDTDCDGDVP"
     gene            13115..13570
                     /locus_tag="PKK95_02610"
     CDS             13115..13570
                     /locus_tag="PKK95_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17127.1"
                     /translation="MRSAISLALLLLAYTLPCTAQEPPWPEPCAQSWTCWLIWDQNPS
                     SDHVVRYEIWSGTTYCGAVFGGRQSIRNPERWINPHNAYWPHPDDGCWEPGHQLLYVV
                     YAVDDEGLISLNPSNAVAFGPQEWACVEAPGCERPCPVRRLPHLPECGP"
     gene            13567..13779
                     /locus_tag="PKK95_02615"
     CDS             13567..13779
                     /locus_tag="PKK95_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17128.1"
                     /translation="MNIGLAGRTRADLREHAERLVRERVPRVLAIAELRREGWRCDLC
                     QIEYAAARRRLRVCPACGRATSLEAD"
     gene            13779..14069
                     /locus_tag="PKK95_02620"
     CDS             13779..14069
                     /locus_tag="PKK95_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17129.1"
                     /translation="MEAGTLAQWGTLALGVLGAGGVMGLAYHAGRMSHRVETLEGAHY
                     TPGAQCAERHDRIGEEVAQCRAAIAALDGKVVGVADKADRLLALFAGVGRHE"
     gene            14066..14539
                     /locus_tag="PKK95_02625"
     CDS             14066..14539
                     /locus_tag="PKK95_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17130.1"
                     /translation="MSDVLAASGIDVERLLIAIRHVETASAPTGLRFEPAWMPRGYRF
                     PLQGRELVGTARSMTAIAEQRWVKWGPWSSASYGPWQILYHAAADLGYDGHPCDLLRL
                     DVCRPWVERLLHRIARQGARTLAEIADAWNSGTHRDAIVPQEYIRRLAAAYAAAG"
     gene            14552..14677
                     /locus_tag="PKK95_02630"
     CDS             14552..14677
                     /locus_tag="PKK95_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17131.1"
                     /translation="MSRRPDEIVDRIPRQYRGWLLYLAVIGVLVLVGALLQRACG"
     gene            14682..14891
                     /locus_tag="PKK95_02635"
     CDS             14682..14891
                     /locus_tag="PKK95_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17132.1"
                     /translation="MGVHRGERGYRLALWSLLCGTALGAAGIWRGVDLVGLAAYTTGM
                     IGAALGTTAWRAHRESRAATGATQP"
     gene            14888..15106
                     /locus_tag="PKK95_02640"
     CDS             14888..15106
                     /locus_tag="PKK95_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17133.1"
                     /translation="MTVTQQIRAQASVRLADAIAPLEDLLARELHDAPDDAYMGQPGD
                     GCARLTVRQLRDLVSAVRAGRAALGVSP"
     gene            15103..15888
                     /locus_tag="PKK95_02645"
     CDS             15103..15888
                     /locus_tag="PKK95_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17134.1"
                     /translation="MTTWIQRAALVALTAACAVLGVCAVVRGGQLERERTAAREARAA
                     ADAAALRESGALAAHEATVREMREVLADADARLREALEGAQRAVPGARVTSVLTGSTG
                     PVTAPDQREAGPGAAACDTPPSSQPAAAPISDTCLFHAGDTGEVRADVAELVTEAGAS
                     ALVGALSCWRTSPPPAQEILGGPLRLDVSRWVTRRDEARQPATWSLEVRAGLGTRGVE
                     GGATLYGRGRLGGWAQSWWEPGAAPGESAWGAAGGVSLRIGRR"
     gene            complement(16060..16437)
                     /locus_tag="PKK95_02650"
     CDS             complement(16060..16437)
                     /locus_tag="PKK95_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17135.1"
                     /translation="MEDALTITMTGRAPVKILKTDWPVIASSRGDSYGGTDGGRHQQA
                     LTRGELDAYRLTVRQREDGRTIVYGVLDGAPAWTGTHSRRAGELLDPGDDIAAAIQRV
                     GVECRLPEPVIRACIADLPAEEI"
     gene            complement(16531..16731)
                     /locus_tag="PKK95_02655"
     CDS             complement(16531..16731)
                     /locus_tag="PKK95_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17136.1"
                     /translation="MTRTATPKQVSYLMVLLARAGYDTRWMSSRYRELGASMRERSGS
                     VSVWLSSLTIGEASALIDRLRG"
     assembly_gap    17004..17103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17104..17427
                     /locus_tag="PKK95_02660"
     CDS             <17104..17427
                     /locus_tag="PKK95_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC17137.1"
                     /translation="RTPAIGGDGTPTALDKKDGTNILTAVLSGGSVIDDMTTVPLKFS
                     AERLRAARGDRRPEELALAADVSAQTIRNWENGTYEPDATSLARIAAHLGVAIESFFV
                     PQEAA"
     gene            17609..17989
                     /locus_tag="PKK95_02665"
     CDS             17609..17989
                     /locus_tag="PKK95_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17138.1"
                     /translation="MEPQAENVKGGRTVRVPDGIPRCARCGRPMLESGPVVCSWCEEE
                     RERREEDQAWRDRGGVEFQPCHDAEWYDGQAREMAQYAEWQYSLGHDALGRKLARWSA
                     DYHVMAERIRTITELRQWTLGGNV"
     gene            17989..18411
                     /locus_tag="PKK95_02670"
     CDS             17989..18411
                     /locus_tag="PKK95_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17139.1"
                     /translation="MPDFRTMYDLNWIYAFDLKGHDVTVTIARVEAKKIKNAEQKEQK
                     KPVVYFRESHDQRGLVLCKTNAKTIAGLYGNNTDQWIGKKITLYPTMVSAFGAECEAI
                     RIRPRVPSGKPGELHEVPEAPQPEAPELAMEDHEAAGQ"
     gene            18408..19172
                     /locus_tag="PKK95_02675"
     CDS             18408..19172
                     /locus_tag="PKK95_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HOC17140.1"
                     /translation="MIAPVRFSALKRIAQSPAHYRYHLEHPPEPTPAMRIGTVAHAVV
                     LGAWRPYAIWSGGRRYGTEWDAFRAEHQGQDIYSAEEWAEGERIAAAVAADPVAAPLI
                     ARARWREQEVAFRIGSRRCGCRIDASGRGLVVELKTCREASPTRFPYQALRMGYYAQV
                     AWQVDALDRRRRRRIREAYIIAVEQKPPHVVQVYEVTPASLDYGRRTYALWFERLLNC
                     EASGEWPGYVQSVVPLEPPGEAGDIALSIGGEEIAV"
BASE COUNT         3163 a         6239 c         6892 g         2815 t
ORIGIN      
        1 catcctggtc gggctcgcct gtcctcgcgg cgtggagccg gaagtctggg actggctgac
       61 ccgcctgctg ccggcgcggc tcgggcacac gctgcagatc gcccgcgtgg acggcgaggc
      121 cgagatcgcg tgggccaata accagctcgt gcagaagctg ctgcgcgggg actggacgca
      181 cctgtggttc gtggccgccg acatgcggcc gtacccgcag acgcacacga tcctcgaggc
      241 gacggcggac gtggcgatcg gcgtctcgtt cgtggtcgag ccggacccgc tcgtaccgca
      301 gttcacgcac ctctggccga acgtttcacg gagtgacgac ggcctcaggt tcccgccgct
      361 gaaatgggac gagctcgccg acggcccgct ggacgtagat ggtgggggga tgcactgcac
      421 gctagtccgg cgcgaggtgt tcgagagccc ccgcgtgcgg acggatggcg gcgcctggtt
      481 ccgcacgcgg cgcgacgcct tcgaccggcg aatgttgact gacgacgcgg acttctgctg
      541 gcgcgcgaag caggccgggt tctcggtccg cgcatacccc ggcgcgctct cggggcacgt
      601 caagacctgt gatgtcggcg gtgtgtggac ggccgcgcgt gtccgcgcgc tgtcggaggt
      661 gtgatcatgt cgatcgcgac gacgattcaa ctgctcgact ccgcggcgct cacggcggac
      721 gggaccaccg actacttcaa gatcccgacc gcaacgatgg gcgagctgca cgtcgaggtc
      781 gtgagcggct cggggacgat ctcgggcttc gacctgtggg cacagtcgca gtccgaggcg
      841 ggggccagcc cggtcgacat cctgtgggac cgggcggtga agaccgccga cgccgcgacc
      901 gagggcaccg cgcgctcgaa cgtccgcgat gtcgtcgatg ggaagatcac gaccaccgcc
      961 gagtacttct gggccaagat cgatgcgctg gcggcgtcca ggttccgcct gaagtggaag
     1021 ctgtccggga cgtcgacccc gaccctgacc ctcaatgcct ggctcgtggt gaagtaatgg
     1081 ccgcccggcc gatcacgacc gcggcggtcg tgctggcgaa gaaccccggg atcgactcgg
     1141 cccagatcaa tacgaccgag ctctcgcgct gtatcgacgc ggcctccgcg ctgctcgaga
     1201 agtggtgcaa ccggatcttc gtcgaggcga cctatgccgc gctgcacagc ggcgagcggg
     1261 cgctgcggat ccgggacgac gagggccggg ctgggacgcg gctctacctc gcggacccgg
     1321 tgacgcgact gctgactatg ccggtctccg cggtctcgag ctgcaccgag gacgggaccg
     1381 cgatcacgac gttccggctg tcgacggcgc cgaccttcac cgacggcgat tacggcgcgc
     1441 tggtgagcga cgcgcgcggg atcgtgacgc gggtctcgat cgcggggggc gagatcacgc
     1501 cggtcccgtg ggcgcccggc gtcgccaaca tcaaggttgc gtacaccgcc gggtacaaga
     1561 cgtcaggatc caccgaaccg ctgatcccgg cggacctggt cgaggcctgc tgtcacctga
     1621 cttggctgct gttcaatgag ggtccgcgtt ccggggccga gatcatgtcg gaagcgggca
     1681 ccttcgcgaa ctactcacgg ctgctggtcc cgtctgtggc ggacgcgatc aagcgctatg
     1741 cgctgccgat gtcgccgatg acgctggagg cctgagtgca ggttttcgcc acgctggacg
     1801 acctggatgc gctgctgcgg atgccgcagc tgtcggtgaa ggagatccgc aagtgcgccc
     1861 ttcgtggcaa cgatgtggcg attggcctgg cgatcgatga gctccggagt gaactgaacc
     1921 gcgaggagcc gcgctacttc cgccggcgcg cgcgcgggct cctgaaggac ttccgccgga
     1981 tggcccgcct ctaccgcgca acgttcagcc gcggacttgg ctccgctctg gccgcgcggg
     2041 ccgagctggt ggccgtcggc ctgggcgggt cacggatgcg ggccgccgcc ccgctcgagg
     2101 tgggcggcaa gtaccagcaa ttcgtcgagg cccacacgcg gcgcgtgaag ggccgcgacg
     2161 tccgcgagcg cgtggcgacg gcgagcggcc ggcgtcgcat gacgaccatc gcccagggtt
     2221 acacgcaggt gcgggagcat ctgcggatgc ggccagagga cgcgcaccgc tacctgctgc
     2281 gcgcggtgca ggcgaagctc agcgtgatga tcgtcccgac gagccgggcg ctggccggcc
     2341 tgatcgcgac gggcaaggtc ccaaccgcaa agactctccg ggcgggcctg tgaacctgtt
     2401 ggtcaatccg ggcttcgagg ccggcgatac cggctggacg ctggcgaccg gcgcgacggt
     2461 cacctctgcc tacggcgcgc acatgggggt gaacgcctgt cgcctgcgct ggaaactggg
     2521 gacgatcatc ccgcccgtgc catccatccg cggcgcggcc tcgcaggaga tcgcgagcac
     2581 ggccggcctg gtgtacgagc tctcggcgtg ggtcgcgctg gaatcggggc aggccggcgc
     2641 gcggctgcag gtgcaaaacc cagccggcgc agcgtgggaa gacgtcgaca cgatcctcta
     2701 caccgacctg actgacgact gggtacggct ctcgggatcg gtcacggcag ccggcgacac
     2761 gctggcggtg cgggtcgtgg tctacgaggg cgcgcaaagc aagggctaca tcctggccga
     2821 cgatctgatc gccggcgtcc cggtggagat acagctcatg ccgcgaggac tgcgaaaggc
     2881 gtacgtcgac ttctacaaca agatcgcgtc gatctcgacc gcggacgggt actaccacac
     2941 ggtctcgacg atcatcccgc ggatggcatg gccgaacgag cagaatgcgc cgcgcgtgtt
     3001 cccgtacgtc tgcgtgcccg cggatcacca gctcgaggcc ggggattatc cggaggtcga
     3061 cagcggatcg gttcgggtcc ggctgcgcca gccggtgatc gtggtgctca agcgcagctg
     3121 gagcgatgac gccgagaccg gggccgcggc cgacgcgctg aactggcatg acgacatcct
     3181 gcgggcggtg atgccggccg cgcagtccgg tgcgtggaat gccgggagcg tgatcatccg
     3241 cgacatcacg ccgcacaagt tccgcgtgct cgccgacccg gtggagggcg gcgcgccgtt
     3301 cgtgtcggtg gagttcctga tcgacctgat cgtctcgcgc gacgatctcg gtccggaggc
     3361 ctcgtgatgg cgaaatggct gcggttcacg ctgccgaaag gaatgcacac ctgggcggtc
     3421 cgccgcgacg acccgcgcgt gctgatctcg gggcacatcg tggacggggc gcagttgccg
     3481 tacgacgacg tgcatgacgc ggatgggtac gtcctgcgcg ggcaggcgga aatcgtggaa
     3541 gttggtccga aggtggccga gcccaagccg gagaccgggc cggccgcgtc cgactagagt
     3601 cgcggcgccg agccgcagga ggtgattcat ggcactggaa ctgaagaaca tcgcgatcgg
     3661 cggcacgacg ctgagtctgg gcgcatatgt gaccgccggt ggggccgggt cactgaccga
     3721 cgtcggcctg ctgaaggacg gcagcgagct gaacatcgcc aaggaaatct acgaggtcaa
     3781 gtcctcgtcg gcgaacggcg tgctgcgcgc gatcccgatc gacagcacgt acgagctcaa
     3841 ggcgatcctg caggaggcga cgctcgacaa catgcggaaa gccctgggcc agccgagcgc
     3901 gaacctgacg ggcaccgcgc cgaacaagac cctgctcgtc ggcgacatgg ccgaggagta
     3961 tttccagggc acggtctcca ccggcggcac cggcggcacg ccggccacgc ggttgatgac
     4021 gttctggaag ctgtacgtca aggccgtcga gacgctcgcg ttcaagaagg gccaggagca
     4081 gatgtacggg atcacgttcg ggatggtcgc cgaccagagc gtcgcgaccg cggacaagtt
     4141 ctacaaggtc gtcgacagcg gcggggtcgc gtgatcggcg acttcgacag cggccggcgg
     4201 gtcgtgctcg cgggagggcg gcgctacctg tgccgtcctc ccaccgtccg gaccgtcgac
     4261 cgcgtgctga cgctgttcct cacggaggtc tctgccctgc acgcggcggc ggccgacgtg
     4321 tcgctcacgc cggaggagat cctgcgcgtg ctctccgggg accgccggct ggccgaggtg
     4381 ctggacacct gcgtcgaatt gtggggcgcg gcacccggcg agctcgtcga gctgctggcc
     4441 gacaaccgcg atctccaggt cgagctcgcg ttggcggtcg tgggcctctg cgacgcggcc
     4501 tggatctggt cgacgctgca cctggccgag gcgctcgagg cgcaggagca ggccccgatc
     4561 gggccggagg acgcgaccac gcaggacgcg gtgttgtgcc ggatcgcggc ggcgttcggg
     4621 aggccgccgc acgagctgct cgactggccg tacggtgcgt tcgtgcgcgc ggtgcagctg
     4681 gggtacggga gcgatccgct cgcggcccat ccggccatga cgaccacgct ggcggacctg
     4741 gaggtctgag cgatggccgg caatgagctc aacgtcaggc tccggatcga gagcatcctg
     4801 aaagagaagg ggctgctcga cctgaagcag cgcctcgagg cggccgagaa agaagtcgtc
     4861 aagctcaatg cgacggccgc tgcgagctca ggcgcattcc gtgcgatggg cacggcgatc
     4921 aaaagcgcgc tcctgccgct cgcgggtttc ttcgccgtcg gcaaggtggt ctcgttcctg
     4981 taccaggcgg ccgaggggca gcgcgagatg gaacgccgga cgatggcgct ctcgcgccag
     5041 attcaacagc tcggcgtcga cgtcccgcag gcgatggagc gggtagagga gttcaccacc
     5101 ggtctcgcga atctaacggg tgtggtggac gacgagtcga tcccggcgct gacgcaactg
     5161 atcggcttga ccggcagcgt cgagggtggg atcgccggcg tcaaactcgc cgccgacatc
     5221 gccgcgtccg gggtgcgcga ctttgccggc gcggtggaac tcgtcgcctc gatactggcc
     5281 ggtcgcgcga atgaggcggc gaggcagctc gggatcaata tcaaggacgt caacggccac
     5341 gcgaagagca acgtcgagct ggtgagcgag atcatcgaga agcaggaagc actcgccgag
     5401 caggccgcca agaacaccga acagacgagc aagctcggcg cggcctggga cttgttcggc
     5461 gacacgatcg gagactatgt ccggcccgcc ctggacgttc tgaagtccgt actcgcagca
     5521 gtgctcaagg gcgtgctgat gacgatcgac gggatcacga tcgtcgcgca ccgggcgata
     5581 cagttgccgt cgcggatctg ggccgggatc aagggcgacc gcgagaagct gaagcagctc
     5641 gaccaggaac tgctcgacgt cgagatcgag ctggcacaaa agtacatcga cgccgagaac
     5701 aaagccctcg aaacgacccg tctcagcgcc gaggagaagg cggcgctgct taatgccgcg
     5761 cggctcaagg atctggaggc cgaacagaag gcggccgagg agcggcggaa gctggccgag
     5821 aaggaggccg aggagcggcg gaagctggcc gagaaggagg ccgaggagcg gaccgagaac
     5881 gagcgggagg ccgagctcgc gctgctcgag gcgaagatca agatcgcgga ggagggcagc
     5941 gccgagcggc tggccctcga gatggagcgg ctggacaagc tgatggccgc cgagctcgcg
     6001 gcggcgaacc tgaccgagca ggcgaagctt gcgatcgtcg ggaagtacgc gctgctcaag
     6061 caggatctgc tcaacaaggg ctatgaggac caggccaagc gggacgagaa ggccgcgaag
     6121 gaaaagcgcg acttcgacga gggaatccgg aaagacctgg cgcggctcca gctcgagctc
     6181 gccgaggacg ccgcggacca gcgcgcggcg gagctcgccg cggccgagca ggattacgtg
     6241 gacgactaca accgcgcgct gaagctgggc ggggacatcg cgaacgtcga ggagaagtac
     6301 cggagaaagc gcgcgtcgat caacaagaag tatgacgacg cgcaggagaa ggtcgacaag
     6361 gcggcggcgg cgaacaaggt gcagatgtat gccacggcgg cgcagatgtc gatcgccgcg
     6421 ctgcagacat tgttcggtaa ctcgaaatcg cttgcgatcg cgcaggcgat aatcgacacg
     6481 ttcctgggcg cgaaccgtgc gctgggggaa ctgggaccac tcggcccgcc ggtcgcggcg
     6541 ctgatcatcg ccgcgggctt gaaaaacgtg atgatcatca agaagacgaa tccgcagaag
     6601 gaggggggcg gcggggcggc cgtaccgcgg atggccggcg gcggcatggt gctggggacg
     6661 agcctggtgc aggtcgccga gacggcggcg gctcgcccgg aactgatcat gccgctgcgc
     6721 tcgcgggtca ccgaggagct gctggccgcg gcgctggccc gcgcggcgca gagcccggcc
     6781 gcgcagtccg ccgggttggg tgccggccag agcacgatca tcaacattcc ggtgacggtc
     6841 aacacggcgg tcccggagcg ccgcgcgctg cgcgagctgc accggcaatt gcagcgggtc
     6901 gcccgggagg acctggcgcg gagggcggga tgagtccgcg gccggcgttc cgcgtgctgc
     6961 tcaccggccc cgacggcgtc gaggacgaca tcacgacccg cgtcgacgtg tcctcgctcg
     7021 gccggctgac cgaggagctc gaggacgacc tgctccagct cgtccattcc gacatcagcc
     7081 tggagctcga cgatgctgat ggctgggcca gcgcccggct cggcgcggcg acgcgcggcg
     7141 aggtctggga ggtggtcatc gagcgcgaga ccgggcgccg gcggccgctg tggacgcgca
     7201 tcttcggcgg cgccctggac gtgcccggct cgatcgtgat cgaccgcaag gagcgggtgg
     7261 tcgagctcga ggtctggtcc tactcgaagc tgctcgagct ggccgcggcc tcggcgctcg
     7321 cgcgctcggt gacgaaccat atggcggacg cgacggccgc tgcacttacg gcgacggtct
     7381 ccccggacac gtcgctgctg gtcgccggcg accagatcac gctcaccggc ggggggcact
     7441 cggacagccg cgtgatcgcg atcgtcaata gcgcaacaga ggtcacggtg acggaggcct
     7501 ggtcggcgac gttcacctcg gcgaccctga cgctcgacac gccgtacccg cgcagcgcgg
     7561 tggctgagga cctggcgcgc gacctgtacg cggccgccgg catcgcggac gcgacgatcg
     7621 acgtgggcgc agtcctatcc ggcgtgctgt tcccgaccgc gatgagcgct gacggcctcg
     7681 ctggcgggac gccgtcgtcg ctcgtggaat tcggcggcaa gctcctactg tatctgggcg
     7741 gcaagcggta cgaggcgacg agctcgagct ccggctggac cgatgccggt gccgacacgg
     7801 tcaagctcga ctggcaaccg tacctcgcga ccgagccggc cggcctgcag ccgggcaccg
     7861 cggcaaagat ctacgactac gtcgacgcgg cccatccgta ctacgagctc aaggtcgaca
     7921 agagcggcgg gtcggaccat ctccggatct ataaggacgg cgcgccgctg gtcaccgtgg
     7981 acagcgtcgg tatcagcacg ccgaccccgg acggcgaatt tacgttctac acgtacgagg
     8041 tcgccgggta ctggggcgag gtctgggtct cgtaccgggc cacgacgaaa acgcgctccc
     8101 gcgagtggga cgcggacttg ggcgagttcg tctgggtcga tcacgtgctc tacaagaacc
     8161 gcacggtccg gtatgcgacg gacgggacgc tgctgcagac gatccaggcg gcggggccgc
     8221 tgcggttcgt gcgggcggac gaccggatgg cctggctggt gacccacctg ggcggggacc
     8281 aggacgacct gcaggcgctc gcggagccgg cggtccatct ctggtaccac ggcgcgtcgc
     8341 agggcgtgct cgcggcggcc agcgtcgata tgtggacgtt ccgcaagatc ggtaccgcgt
     8401 actacgccgg gatccagggc ggcgacgtcg tcctgtggga tgccgtcacg cgcgacctgc
     8461 tggcgatcta cccgctggtc gcgactcccg ggggcggggc gctggcctgc gtgttcacgg
     8521 cgggcggcac gatcgacgcc gagtacgacg gctggaccag cggcatgtac ttcagcctcg
     8581 cgctagctgc gacgaacgtc gtgccgtacg ccgatttcgc cgacatgtcc tgcgccgccg
     8641 cactgcgcga gctggccgtg gtgacgatgt cgcgcttctt cgtcgaccag tatcggatcg
     8701 gttacctgat cggccgggcc gccgactgga tcaccggccg cgccgcgatc gagccgggcg
     8761 tgccgctcga gcagcaggtc cggccgatat gggacagcct gtgtcgcagc gtcgccgtga
     8821 ccggcgaggc cgaggacggg accgagatcg aggtcctggt cggtgacgcc ggcgactcac
     8881 aatgccgcct cgaggtgtcc acgcaactgc cgatgacgag cgggctcgcc ggcgttctgg
     8941 ccgcggccta cctgtcgtac ctctcggtcc cgcggcagca ggacgaggag accatcgacg
     9001 agcccgaaac cggcccggtc cgggtgctgg atcgcgtcgt tcgggacggg cggacctggg
     9061 tcaccctgcg ggtcgaaagc gacctgcggg accgcgagca ggagctgctg ctggtcgagg
     9121 aggtcgcgtg agcgggtacg cctggtggcc gatggtgcgg atcacgccgg cgaacggccc
     9181 ggaggtggtc cgcgacctga agagcgacct ctcgactctc tggggcatcg ggccgacgaa
     9241 gatcgcggtc aagcacgcgc cgcagatcaa gcagcggcag gacgtgaacg accgcgagcg
     9301 cccgctgctg aagggcttcc gggtcgaggt cgatctgacg ttcgaggtct cggacgagat
     9361 gacggaccac gacatcctgg ccgagatcgt gacggcctgc ctcgaccagt accaggtcgt
     9421 cgagctgtcg atggacggcg ggctgacgta ccgcggggtg gtgctgaccg actacctcgg
     9481 gccggacccg ctgggcggca agcccgtcgc cggcgcgaag tacgagctgc acttccggtg
     9541 ccgcgagctc gtgaccgaac tgccggcgat cggcaccggg aactggtgag gaggtagggg
     9601 ggcaccggtc tgcagccggg gctccgggat ccgcgcccgg ggccagcccc ccgaaggttc
     9661 gacgcgggcc gcggtacgcg gagccgccgg aggcgcgagc gatgcgccga tggctgctgc
     9721 tgatcctggc gctggcggtg acgcccgcgt tttccgcagt ccagaccacg acggtcgagg
     9781 gctacgtgct caacccgtcc ggcggcgcgc acaccgggac gatcgtctgc acgctctcgc
     9841 acgccggagc atcggacgac ctgacgaccg gcgagtcgga gcgcgtggcg tcgaagtact
     9901 cgacgacgat cgcgtcgagc ggcgcggtca acttcgcgct cgtgccgaac gacgcgatca
     9961 cgccggccaa tacctcgtac acctgcgtct tcaccgtcgg cagcggggcg acgcggtcga
    10021 gttggacgga gcggtggagc gtcgggacca gtcccgatcc ggtcgacatc ggcgacatca
    10081 cgcggctcga cctggccgac ggcctgccgg tgacgctgga cgcgctgacc tgcaccgact
    10141 gcgtgacgct gggaaccgaa accatcggca actacgcggg ctcgcccagc gagggcgggg
    10201 gcgcgctgtc gctccaaggc gcggatgctg cgaccggcgt accagtggac gcggacgggg
    10261 atgccgtcaa cgaagctagg attcgcgcgg gcaagatcta tggcgaaacc gtcaatcgca
    10321 cggtgccgtg ttgggatctc ggcggcggcg cggaggactc gccggacggc acatgcgatg
    10381 tgtttttcga cagctatcgc ggggccgtgc gcggcgacgg gacgcccctg ttcatggggc
    10441 cggtttcgat ctttgcgaac tacggcgact tccatccgct gggcgtcggc tcgaaatact
    10501 tcgacaggct cgactgggag ctgacccagt gcaaagccta tgggatgccc tgcggcgtca
    10561 atgttcagac cgcctcccat tacaatccgc ccattcgtct tcctgatgcc aacatcaagg
    10621 aaatctacga cgccggcgtc tcggtcggtt cgcacaccga ctcgcactgc gcgatgacgc
    10681 tgttgaacca gagcttttca ccaaactatg cggtcctcga aaactctgga ggccggtaca
    10741 cgaacggctg tggtacttcc tcgccaacgt gcgttcacac cgggacggtg tcagttaccg
    10801 aaggcagcgc gaatctggtt ggcacagcga ccaatttcgc cggtgaatgc aacgacaaca
    10861 cggacgattt cgcacctccg ggcgggagga tcttcatcga cacggacggc gacggcaccg
    10921 tcgagttcaa cgtccgcgag tgctacggca cgcagaccgc ctgcaacaag gacgccgatt
    10981 gcggtggccc gaaatgcgtc gcggatttct cgtccgacat cctgaccgtt accgatgcca
    11041 cgcaccttgt cctgcgcgat gcgctgccca ccgatactga tcgcgacggc acgacagggg
    11101 agacgttcgc aacggcgatc tttgagatcg tgtccggccc tggccaatgg cggcaggacc
    11161 tgaagacctc ctgcgccgtt atggagaatg cgattggtga tccgggctac aagtgccgat
    11221 ggtttggcgc tccgggtggc tggatcggca gcgagattta cgagacggcg tatgccgcct
    11281 ctgacatcga cgcgggcgtg ggcagtggct ccggctggga taatcaggag ttcgaggtgg
    11341 cgggcgcgcc aactactgcg acggtgctgg agaggttcca cattaattgt ggcgcgagcg
    11401 ccgactcgat caaggccttc atggagacgg cggccaagag ccgcctgaac ctcttcttct
    11461 ccggtcacac gcctatcgct attgatgatg acgaatgtcg tggtcccgat cacgttctcg
    11521 gcaactacga cgacgcatcg gatgcgtggt cgcaagccaa ctttccggcc taccttcaga
    11581 agtgccgcga gttgatgcgc gacgggcgcg ggatctgcct cgatccatac cacgcgggcg
    11641 tgctgatgcg ggcctggtcc ggcaaccagc tcggcgtgaa tctgatcggc aatagcgacc
    11701 ttgcggattt caactcgggt tggaacgtgg gcagcggcaa cgtgaccaag cggttcccgt
    11761 gggaagaccc gaagggcacc atcaacatta acgacgcgcc ggggtccacc gatcaactgg
    11821 actacgacgc aacgaaccaa tgggccgtgt tcaagtacga gaacgcggac ggcgacggcg
    11881 ttgcggacga cgtgtcgatc tcgcagcgcg tgaagctctc gtccggctac taccagattg
    11941 ggatgtggta caagctgctg caggacataa ccggggccaa caacgtcgcc ggcgtccggc
    12001 tctgtattgg atctacgtgg tgggcgttcg gcggtaaaca aggcgtgtcg gacctgaccc
    12061 cacaatgggt cgagcattac aagggcgcgg acaacagcgc aaccgggacc acctatttca
    12121 accagaaggg ctgcacgaac tttcaggtcg gcaatgcggg caccgaagat cagatcatcg
    12181 gttacttcta cgttccgccc gagcaggacg ggatcgtgca ggacatctac gttgagttcc
    12241 gcgactgcga cggcgatggc acctcgaacg actgcggcaa cgacccgtat atcgcattcc
    12301 gcgacgtaac actggtcaag tacgtcccgc agccaaccga ctcccgttat ggcgggatgg
    12361 gggttacacc tcttttgtcg gtcacgaagg tgccacacaa cagcgcccgc actgattctg
    12421 gagcgtggac gtggctgtgg acaccaaacg atcagaacag cgggcagtcg gcgaatccga
    12481 cgacagattt cgtcaccgcg cgcacgattt atcctgctgt cggcccctac acggtattcg
    12541 ctatcggcaa gtacaacatc aacagcgacg acacctggcg cggcaacgcc tcaccgatga
    12601 tgcgtctcgg caacaccacc atcggcggcg cgacgggaac cgcagcggga tatggactcg
    12661 gacagacgat ctacaagggc agcaatttca gcggcgcgtg ggtcgggcga tctacgacct
    12721 caaagggcgt tgactggctg ttgaacctgc ccgccacttc gggcgcgaca gaaccagcga
    12781 tccgctataa ggcgctcaac tataccaagg tcgataccga cgctatcgca tcgtggggcg
    12841 tagggggatc gcataaggcc ggcgacaaca cccaggacga ggtggcgcaa ctgtggggcg
    12901 cggggacgct ttcgctggaa ggcggactca agctcggcac cgatgccgag gaagcggtcg
    12961 ccaccggcac caacagcctg tgtatcaagc gcaaaaccga ctgcggcgcc agcgagtgcg
    13021 tgttcatcga cctcgactgc aacggcacgg tcgacgcccc agacttctgc atgcatagcg
    13081 atggcattga caccgactgc gacggtgacg tgccatgaga tccgccatct cgctcgcgct
    13141 gctcctgctc gcgtacacgc ttccgtgtac cgcgcaggaa ccaccgtggc cggaaccgtg
    13201 cgcccagagc tggacctgct ggctgatctg ggaccagaac ccgagcagcg atcacgtcgt
    13261 gcgttacgag atctggtccg gcaccaccta ctgcggcgcg gtgttcggcg gtcggcagtc
    13321 gatccgcaac ccggagcggt ggatcaaccc gcacaacgct tactggccgc acccggatga
    13381 cggttgctgg gagccggggc accaactgct ctacgtcgtc tacgccgtcg atgacgaagg
    13441 actgatcagt ctgaatccat ccaacgccgt ggcgtttggc ccgcaggagt gggcgtgcgt
    13501 cgaggcgccg ggctgcgagc gaccgtgccc ggtgcgccgg ctgccgcacc taccggagtg
    13561 cgggccgtga acatcggcct cgccggacgg acccgcgccg acctgcgcga gcacgcggag
    13621 cgcctggtcc gcgagcgagt gccgcgggtg ctcgcgattg ccgagctgcg gcgcgagggc
    13681 tggcgctgcg acctgtgcca gatcgaatac gcggcggcgc gacggcgcct gcgcgtgtgc
    13741 cctgcctgtg ggcgagccac atcactggag gctgactgat ggaggctggg acgctcgctc
    13801 agtggggcac gctcgctctt ggcgtgctcg gcgccggcgg ggtcatgggg ctcgcctatc
    13861 acgccggccg catgtcgcac cgcgtcgaaa cgctggaggg cgcgcactac accccggggg
    13921 cgcagtgcgc ggagcgccac gatcgcatcg gcgaggaggt cgcccagtgc cgcgccgcga
    13981 tcgccgcgct cgacgggaag gtcgtcggtg tagccgacaa ggcggacagg cttctcgcgc
    14041 tgttcgccgg ggtcgggagg cacgaatgag cgacgtactc gcggcatccg gcatcgacgt
    14101 agagcgcctg ctcatcgcga tccggcacgt cgagaccgcc tcggcgccga ccgggctgcg
    14161 gttcgagccg gcgtggatgc cccgcggcta ccgtttcccg ctccagggcc gcgagctggt
    14221 cggcaccgcg cgctcgatga ctgccatcgc cgagcagcgg tgggtcaagt gggggccctg
    14281 gtcctcggcc agctacggcc cgtggcagat cctctaccac gccgcggccg acctcggcta
    14341 cgacgggcac ccgtgcgacc tgttgcgcct ggacgtctgc cgcccgtggg tcgagcggct
    14401 cctgcaccgg atcgcccggc agggggccag gacgctcgcc gaaatcgctg acgcgtggaa
    14461 cagcggcacg caccgggacg cgatcgtgcc gcaggagtac atccgccggc tcgcggcggc
    14521 ctacgccgcg gcgggctagg gaggaacacc catgtctcgc agacccgatg agatcgtgga
    14581 ccgaatcccg cggcagtacc gcggctggct gctgtacctc gccgtgatcg gcgtgcttgt
    14641 gctcgtcggc gcgctgctcc agcgcgcctg cgggtaaccc tatgggcgta catcggggcg
    14701 agcgcggcta ccggctcgcg ctctggtcgc tcctgtgcgg caccgcactc ggggcggctg
    14761 ggatctggcg cggcgtggac ctggtcgggc tcgccgccta cacgaccggg atgatcggcg
    14821 cggcgctcgg gacgaccgcc tggcgggcgc accgcgagtc ccgcgccgcg acgggggcga
    14881 cacagccatg acggtcacgc agcagatccg cgcgcaggcc tccgtgcgcc tggccgacgc
    14941 gatcgcgccg ctcgaggact tgctcgcgcg cgagctgcac gacgcgcccg acgacgccta
    15001 catgggccag cccggtgacg ggtgcgcgcg gctgaccgtg cgccagctcc gcgacctcgt
    15061 ctccgcggtg cgcgccggcc gcgccgcgct gggagtctcg ccatgacgac gtggatccag
    15121 agagcggccc tggtcgcgct cacggcggcc tgtgctgtgc tcggcgtctg cgccgtggtc
    15181 cgcggcgggc agctcgagcg cgagcgcacg gcggcccgtg aggcgcgtgc cgcggccgat
    15241 gcagcggcgc tccgcgagtc cggcgcgctc gccgcacacg aggccaccgt gcgcgagatg
    15301 cgcgaggtcc tggccgacgc cgacgcgcga ctccgcgagg cgctcgaggg cgcacagcgt
    15361 gccgtgcccg gcgcacgggt cacgtcggtg ctcaccggct cgaccggccc cgtcaccgcg
    15421 cccgaccagc gcgaggccgg cccgggggcc gccgcctgcg acactccccc ctcctcccag
    15481 ccggcggcgg ctcccatctc ggacacctgt ctgttccacg ccggcgatac gggcgaggtg
    15541 cgcgccgacg tggccgagct cgtcaccgag gccggcgcgt ccgcgctcgt cggcgcgctc
    15601 tcgtgctggc ggacgtcacc gcccccggcg caggagatcc tcggcgggcc gctgcggctg
    15661 gacgtgagtc gatgggttac gcggcgtgac gaggcgcgac agcccgcaac ctggtcgctc
    15721 gaggtccgcg ccgggctcgg cacgcgcggc gtcgagggcg gcgccacgct ctacgggcgc
    15781 ggccggctcg gcggctgggc gcagtcgtgg tgggagccgg gggctgcgcc gggcgagtcg
    15841 gcctggggcg cggccggcgg cgtgagcctc aggatcgggc ggcggtaggt cgcgatcgcg
    15901 ccgcggccgg ccgctgtgcg tccgcgggga tgacccacgc gcgcgggccg acgcgcacgg
    15961 caccgcggat gcgcccctcg cggcaccagc gtcggacgag cgagggcgtg acgccccagc
    16021 gcgcagccgc cgccgatacc gtgaggggcg cgggcatgct cagatctcct cggccggcag
    16081 gtccgcgatg cacgcgcgga tcaccggctc gggcaggcgg cactcgaccc cgactcgctg
    16141 gattgccgcc gctatgtcat cgccaggatc gagcagctcg ccagcgcggc ggctgtgcgt
    16201 gccggtccac gcgggggccc cgtcgagcac cccgtagacg atcgtccgtc catcctcgcg
    16261 ctgccggacc gtcagccgat aggcgtccag ctcgcctcgg gtgagggcct gctggtgccg
    16321 gcccccgtcg gttccgccgt agctgtcgcc acgactcgac gcgatgacgg gccagtcggt
    16381 cttgaggatc ttgaccggcg cgcggccggt catcgtgatg gtgagagcgt cttccatcgc
    16441 cgtatctcct gccgccgggt agcgccccgg ctgcgggcgc gcactcgcgc gccggtcatc
    16501 ctgcggggtc gtcggccccc gcgcgccgtg tcagccgcgc agcctgtcga tcagcgcgga
    16561 cgcctcgccg atggtcagcg aggacagcca caccgacacg ctgccggacc tctcgcgcat
    16621 ggacgcgccc agctcgcgat agcgggatga catccagcga gtgtcgtacc cggcgcgggc
    16681 gagcaggacc atgaggtagc tgacttgttt gggggtggcg gtgcgggtca tcgtgtcatc
    16741 ctcctgccgg tcgtgatccc cccggcgcgg ggtctgtcct acacgggaga tagtaccgct
    16801 atcggtaccg cgcgcaaggg gaaatcgaca gaaaatgacg accggtccgg gccgtccaac
    16861 tggtacagat tggcaaccgc gccgctcggc gccccggagc gcgacccggg tacaatccgc
    16921 ccgtgcgtgg tgacggactg gggacgatct accgggtcat tctctgcccc gcgtgtggtc
    16981 gggacaatga aacgcgtgtc cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnncgtacgc ctgccatcgg gggggacggt acccctaccg ccctcgacaa aaaagacggt
    17161 acaaatatct tgacggcagt cttgtctggc ggtagtgtta tcgacgacat gactaccgtc
    17221 cctctcaaat tcagcgcaga acgcctgcgg gcggctcgag gcgaccggcg ccccgaggaa
    17281 ctggccctgg ccgctgacgt ctcggcccag acgattcgga actgggagaa cgggacctac
    17341 gagcccgatg cgacgtcgct cgcgcgcatt gccgcccatc tcggcgtcgc gatcgagtcg
    17401 ttcttcgtac cgcaggaagc cgcatgaccg aaacggtacg tcaatcgggc cttgggcgtg
    17461 tatcgatcgc tcgcacgcgg tgcggttcga gcgggcggct ctggacgagt ggatgcgttc
    17521 catgaggttg cgagcagtgg acctgtagac gactcgggcg cccgggtgga gccggacgcc
    17581 cgaggtgaaa ccactagagg aggtcaggat ggaaccgcaa gctgaaaacg tcaagggcgg
    17641 acgcacggtg cgcgtgccgg acggcatccc gcgctgtgcc cgctgtggtc ggccgatgct
    17701 cgagagcggt ccggtcgtgt gctcctggtg tgaggaggag cgggagcgcc gcgaggagga
    17761 ccaggcctgg cgcgatcgcg gcggcgtcga gttccagccg tgtcacgacg ccgagtggta
    17821 cgacgggcag gcgcgggaga tggcccagta cgcggagtgg caatacagcc tggggcacga
    17881 cgcgctgggc cggaagctcg cgcgctggtc ggccgactat cacgtcatgg ccgagcggat
    17941 ccgcacgatc accgagctgc gccagtggac attggggggg aacgtctgat gcccgacttc
    18001 cggaccatgt acgacctgaa ttggatctac gccttcgacc tcaagggcca cgacgtcacg
    18061 gtcacgatcg cccgggtcga ggccaagaag atcaagaacg cggagcagaa ggaacagaaa
    18121 aagcccgtgg tctacttccg cgagtcgcac gaccagcgcg ggctcgtgct gtgcaagacg
    18181 aacgcgaaga ccatcgccgg tctgtacggc aataacacgg accagtggat cgggaaaaag
    18241 atcacgctgt acccgaccat ggtctcggcc ttcggcgccg agtgcgaggc gatccggatt
    18301 cggccgcgcg tgccgtcggg caagcccggc gagctgcacg aggtcccgga ggcgccgcaa
    18361 cccgaggcgc ccgagctggc gatggaggac cacgaggccg ccgggcaatg atcgccccgg
    18421 tgcggttctc cgcgctgaag cgcatcgcgc agagtccggc ccattaccgc taccacctcg
    18481 agcacccgcc cgagccgacg ccggcgatgc ggatcggcac ggtcgcgcac gcggtggtgc
    18541 tgggcgcctg gcggccgtac gccatctggt ccggtggccg gcgctacggg acggagtggg
    18601 acgccttccg ggccgagcac cagggtcagg atatctactc ggcggaagag tgggccgagg
    18661 gcgagcggat cgcggcggcc gtcgcggccg acccggtcgc ggccccgctc atcgcccgcg
    18721 cccgctggcg cgagcaagag gtcgcgttcc ggatcggatc gcgccgatgc gggtgccgga
    18781 tcgacgcctc cggccgcggg ctcgttgtcg agttgaagac ctgccgggag gcatcaccga
    18841 cgcggttccc gtaccaggcg ctccgcatgg gttactacgc gcaggtcgcg tggcaggtcg
    18901 atgcgctcga ccgtcgccga cgtcgacgga tccgcgaggc atacatcatc gcggtcgaac
    18961 agaaaccacc gcacgtcgtc caggtctacg aggtgactcc cgcatcgctc gattacggcc
    19021 ggcgcacgta cgcgctctgg ttcgagcgcc tgctcaactg cgaggcatcg ggggagtggc
    19081 cgggctacgt ccagtcggtc gtgccgctcg agcccccggg cgaggcgggt gatatcgcgc
    19141 tgtcgatcgg tggggaggag atcgcagtct gaagggagac ggcaatacaa cagaaccacg
    19201 cactgtact
//
LOCUS       DAOPQX010000026        19123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3502_length_19123_cov_4.904080, whole genome shotgun sequence.
ACCESSION   DAOPQX010000026 DAOPQX010000000
VERSION     DAOPQX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 19123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19123
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3502_length_19123_cov_4.904080"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PKK95_02680"
     CDS             <1..450
                     /locus_tag="PKK95_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC17141.1"
                     /translation="RLARVKVGQPLEFTTDALPGRTFTGKVMFINPTIDPASRSAKVV
                     AEVRNTDGQLKGGAFVKGRILVANRSGVLQVPREALQNWNVEQATADVYVVRGDTADR
                     RQVRIGGATATSVEILDGLQAGEQVVTRGSFALKPGDRIAPAKGQGA"
     gene            453..5018
                     /locus_tag="PKK95_02685"
     CDS             453..5018
                     /locus_tag="PKK95_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC17142.1"
                     /translation="MFLSNLSISRPVVATVMMLTLVTLGLFSWRRLPTDMMPEVEIPV
                     LSVVAELPGASPETVEREVSKKIEEALNPMSGVKHVNSISRESLAMIIVEFDLEVEVD
                     SASQEARAKINAIRRDLPANMGDPVIQKFDFQAMPVISVAVRSKTLSARELTTLADRR
                     VKRRLEGVSGVARAKLIGDSTREVAVDLDPARLSALGMGVDEVVAGLASENVNTPLGR
                     LTSAGSEIPLRISGKPASTDGYANMVIARRGGQPIRLADVATITDTIEEPRSLALIDG
                     EPAVAIDITKQTKANTVGVVDGVLAELEALRAEMPAGVELQVVRDTSVFIREAVHDVQ
                     NTLILGGLLTILIVFCFLNSWRSTVITGLTLPISVISSFIVMYFLGMTLNMLTLMALS
                     LAIGLLIDDAIVVRENIVRHLERGEDHFEAARKGTAEIGLAVLATTMSIIAVFVPVAF
                     MKGVVGRFFYQFGLTVAFAVLVSLFVSFTLDPMLSSRWIDPDIERKGRRNVLQRALDR
                     FNEWFERMADGYKRIIGWALDHRFVVSGVAVAAFVAGLGVFTLLQTEFMTPMDQGEFV
                     VKFKSAPGSSIAETRGRVEEVLGALSEFEEVRYTYASIGAGDSDTVRDATVFVKLVSK
                     DERERGLKDFVHDARLRLQKVPGIVLSVQEETDAFQKPLQVVLQGEDIATLKRYAAQV
                     KHELYGIPGIVDIEATMELDLPEYRLIVDRERAAATGLGSAAVANTVGVLVGGQAVTT
                     YEDEEGEGVAVRVRLPQDLRSQVSQIGDLKVTVPSAEGMALVPLSDLVTFTRATSPAE
                     INRRDLSRQVTIDANLDKLALGTASNLALAAANKIEMAPGYKVVIGGDTEMMVESFGY
                     MAEALLLAVILVYLILAAQFESFMDPLAIMFSLPLAVVGMAGTLVLTGDTINIMSLIG
                     LIMLMGLVTKNAILLVDYTKVLRSQGLERREALVLAGRTRLRPILMTTSAMIFGMLPL
                     FFAIGEGAEFRAPMARAVVGGLITSTALTLIVVPVVYSLLDDVTAWLLGRNRKKKVAA
                     VATTALVVLALALTPSAALAQAPAAQGSSAGTALSTLREGMLAAGRPSAGLAGQAASA
                     AQAVQPEAAIPVKTLTLEQALAIAAEQNRDILKAREYQHWVQGRYEQERAAALPRGSL
                     SLSTTRTFDDSMTKLFKDFMPEEGVGGETGIGDIFGGRQDVGIAGFSVNQVVFTWGQV
                     GAAIRAAKLGFGVADQQLRRFQQAVEKDVTTAFYDVLAARELATIARQDLEQRQRHLD
                     ETIKRQSAGTATDYDVLAARVAVENGRPAVIRAENGVRLAREQLRFLLAEPGEVDAAG
                     ALAATIEPPPPYEQVLASALENRPEVAELSGTKGIYGELVTIAKAANKPRVDFSASWG
                     QRRLGLKTISARGTTWNAGLVATIPLFDGWRTKGAVAQAQSDLVRLNFDELKLRDGIR
                     LEVRNALNAVREASEIVAALGGTVAQAEKLLFLAEKGFELGVKTRLEVQDAELNLSSA
                     RANLARAQRDYNVARVNLEWVAGTLGQ"
     gene            complement(5140..5619)
                     /gene="ribE"
                     /locus_tag="PKK95_02690"
     CDS             complement(5140..5619)
                     /gene="ribE"
                     /locus_tag="PKK95_02690"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF000812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOC17143.1"
                     /translation="MGKVFEGRFEAPGKRVAIAVSRFNDFFTKELLGGARDCLARHGV
                     PDDDVDVAWVPGSFELPLVARRLARTGRYGAVVCLGCLIQGDTPHFQLIAAEVTKGVA
                     QAALETGVPITFGVITAETIEQAIERAGTKAGNKGFDAALAALEMMGLMEAIGPDGQ"
     gene            complement(5814..7031)
                     /locus_tag="PKK95_02695"
     CDS             complement(5814..7031)
                     /locus_tag="PKK95_02695"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOC17144.1"
                     /translation="MSGQTLDSIEAALERLRRGEIVIVVDDEDRENEGDFVMAAEKVT
                     PEAINFLARHGRGLICFSTTGERLRALDLRPMVDRDRNTAALGTSFTVSVDAAIGIGS
                     GISAFDRARTVKVLIDPATRPGDLARPGHVFPLEAQPGGVLRRSGHTEAVVDLCRLAG
                     LYPAGILCEIMDEDGSMARLPRLREIADRFGLALVSIADLIAYRRLHEQLVRRESEVD
                     LPTRHGRFRLVAYSTTVDSGIHLALVKGTPSPAAPTLVRVHSECLTGDVFHSLRCDCG
                     EQMEAALDAIEANRSGIFLYMRQEGRGIGLVNKLKAYGLQELGSDTVEANLRLGFADD
                     LRDYGLGAQILRDLGVGPMELITNNPRKIVGLEGYGLVVVRRIPLEVPPNASNLTYLR
                     TKKARLGHLLERV"
     gene            complement(7058..7699)
                     /locus_tag="PKK95_02700"
     CDS             complement(7058..7699)
                     /locus_tag="PKK95_02700"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF006767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOC17145.1"
                     /translation="MFTGLVREIGRVHRLERRGGVTLVEIEAPLVARPPEGPAVGDSL
                     AVNGICLTVVRVAATRLSVEASAETRRVTTLADWRVGGRVHLEPALRAGDALGGHFVL
                     GHVDGTGRLQRMERRGAAASMTVTLARALARRLLPKGSIAVDGVSLTLDEGPFEDRFT
                     VTLIPHTLGATRFASLAPGARLNLELDVLAKAAERVEPGRLTMGALLARGWGR"
     gene            complement(7699..8874)
                     /gene="ribD"
                     /locus_tag="PKK95_02705"
     CDS             complement(7699..8874)
                     /gene="ribD"
                     /locus_tag="PKK95_02705"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOC17146.1"
                     /translation="MNAATAGDRRYMLEALALAARPARRPWPNPPVGALVVRDGAVVG
                     RGVHNGAGEPHAEAAALAEAGERAKGATLYCTLEPCNHQGRTPPCAPAVVAAGVSRLV
                     VSIVDPNPHVAGRGLEVARCAGLAVTLGVCAEEALDLAWPFVVTRAFERPFVWLKTAV
                     SLDGRFAPEGMTPGKGAAYLTGASARHQVHVLRRWADVVLVGSRTAAVDRPRLDGRLA
                     GPDAACPPEDPIPAVLSERLADLPPWAGRPHLAFAGPDAPELAAETVRAGKGTVIRCA
                     AGPSGVEPCSVVARLASLGKHCLLVEGGPAVAASFLRARLVDRWVHYTAPVLLGRGVG
                     WPEWNEPPGAGSEPDGLTLTRAAHCGGDAVAVWDRLSFRDACARLTSLGGANDWERA"
     regulatory      complement(9002..9119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(9245..9826)
                     /locus_tag="PKK95_02710"
     CDS             complement(9245..9826)
                     /locus_tag="PKK95_02710"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOC17147.1"
                     /translation="MNTYQENLGAPVQDVNAGARAPEAAARPSPAPARRDYYDDPRRK
                     SPMLALVLSAMPGLGQVYVGYYQQGFTNALIIAGLIGLLNSGAVNGGAEPFFGLFLAF
                     YWLYNIVDAWRRAVFYNNALAGIGAADIPDDFALVKGGRGSLAGGVALVVVGAVLLMN
                     TLFNMPLDWLEDWWPVTFIAIGAWLAWPSLVKK"
     gene            complement(9840..10745)
                     /locus_tag="PKK95_02715"
     CDS             complement(9840..10745)
                     /locus_tag="PKK95_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOC17148.1"
                     /translation="MDCARFDRRLDALLEGSCSAAEWREAEAHAAACARCRLLLDAMA
                     GLGGSQDAAGAESLADEVLARTSGATCGAARERLGDLVDGDLEGIDRELVEGHLARCR
                     PCAELAAAMARASELLPAFAALQPPADLAPAVIAATSRRPPAPTFDERVAAWLSKLAA
                     RPRFSLEAAYLCTLLIFIIVGNPVAAFRETSARGRELAEPRVVEVLERVSSGPAGLVR
                     EIGEAVAGRAASARRGETGVASRAFALVSPAWEWVSTRVFGPLRDAFTSLVAWVSELR
                     RPNPSTSGRVTETGRAKATDGSGRR"
     gene            complement(10754..11350)
                     /locus_tag="PKK95_02720"
     CDS             complement(10754..11350)
                     /locus_tag="PKK95_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC17149.1"
                     /translation="MERPPGIDLMDLAALIDRCRQGDNLAWEAFVRQFQGRIYSITSA
                     YASGPDEARDLAQDVFIRLYETRGRWAPSDVFVPWLVRVARNLCLDQLRRKKARPPAE
                     DLLADEMVDLAGGGGTPEQELASLNERRLVWRALRSLGKLSREMILLKEIQGLSFPEI
                     AATLGIPIGTVKSRSNRARLELAEQVLSLRRREAGPAR"
     gene            11420..13783
                     /locus_tag="PKK95_02725"
     CDS             11420..13783
                     /locus_tag="PKK95_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOC17150.1"
                     /translation="MVSIRLRHATALVPILAACALAAPGAAWAQAPRQLTAADYQRAQ
                     RLLDANVTPLVLGPAVRPAWLPGDRFTYRTTVERGTEFVLVDVARRTRGPAFDHAAIA
                     AALSRATGAGHDPYRLPFRTFTFVRDGAAISFRAAGKVWECDVAGKACRAAEPAAAAR
                     AGGQRIQPVPQESVSPDGRSAVFIRNDNLWLREVATGAERALTSDGVKDFGYATDNAG
                     WTRSDRPVVLWSPDSKKIATFQQDQRQVGEMYLVSTKVGHPELEAWKYPLPGDAHVPM
                     IHRVVIHVDGPRVVRLKVPPDPHRGTVTDDIKDEEGRLDDVRWAADSSALAFVSSSRD
                     HKQATLRVADPETGEVRDVLEEKVDTFFESGNDRSNWHFLPDSGEAIWFSQRDDWGHL
                     YLYDLVSGKPKRQITSGPWNVAQVLKVDEKARALYFVGVGREKGRDPYYRHFYRVGFD
                     GGDVTLLTPEDADHQVSLSTSGGFFVDAYSTPVTPPVSVVRDLSGKVVMPLEKADTTR
                     LQAIGWKPPIPFTVKVRDGMTDLYGLMFRPTDFDEAKRYPVINNIYPGPQAGSIRSRG
                     FLPAHGDAQALAELGFIVVQIDAMGTPWRSKSFHAAYYGDMGDNGLPDQVAGMKELAA
                     RHPWIDIDRAGIWGHSGGGFASAAAMFHYPDFFKVGVSQAGNHDNRGYEDDWAEKWQG
                     LLVAKPDGSSNYDGQANQNFAKNLKGKLLLAHGTMDNNVPPYLTLLVVDELIKANKDF
                     DLIMLPNRGHAFGNEPYMMRRRWDHFVKHLMGAEPPRPIEFPAPFRP"
     gene            complement(13773..15167)
                     /locus_tag="PKK95_02730"
     CDS             complement(13773..15167)
                     /locus_tag="PKK95_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOC17151.1"
                     /translation="MPAPPSARLGRYDRSIVDGPIGRAVWHLSWPTILQNAMGGLQGI
                     VDHAMVGNFVGYTANAAIGVSWQIFLVIIVFMGSLFTGMAVLVARYAGANEPEKVNRA
                     VYQAFLVAFALALLVLAPLGYVLSPWLLGLVNAAPEVRAQALPYIRTLFVFGFGLLLF
                     YMLGAALRSAGDARTPLKFGVAITVLNVVFNVIFIRGAGPIPAMGTLGAAIGTVLATT
                     LVGIVAIRMLLTERLVVGFPKGDRRPDWTMIRSLFRFGLPAGIQGVAMNVAGLLLLRF
                     IGSLPESAAAQAAYAVGYTELFSMITWTSVGLMGAAAAAAGQNLGAGRPERSVEAVRK
                     AAFGGLLIAAGVGTLFVLIPEKLLWAFGLTDPAVTRLGSELLGYLAFSGFFITVALTY
                     TGGLQGTGDTRSPLYITIVSQIVVPLGTCALLQSSGNLTSRGIWTAIVLGHVTRATLS
                     VVRFGQKKWVQIRV"
     gene            15438..17345
                     /locus_tag="PKK95_02735"
     CDS             15438..17345
                     /locus_tag="PKK95_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC17152.1"
                     /translation="MTFGLLEHLCLSVLPAWRESSLLLVPSSRAAALAVTLAGYAVVG
                     ALLGGAAGAGARLLGWGGGDAPSGRRAAADAATATLVLAWTVHLAAARSFSRSAYLGL
                     AVSALLVAALVARTGWPHGRVPRVTAWLVSPAGASVCLLAPLVFRDRLHLAALGEHLG
                     TSAGVFKAGAAAAGTSLLVLLLVAGALFHRGTGRHTGRARLAVLLALALTLGASSVLL
                     DRAALSRLSAGGAAPAPRESTAPPRPTGILLLTLDAVRADHLPVYGYPLDTSPNLASL
                     ARHATVYLNARAASDMSLGTHASLFTGKYPSRHGAWVGGAFPVGRPLAASERTLAERA
                     AEAGCIRFAAVANGYFLNTAYGLSRGFQLFDVPRADRLELRLRLDFAARRLAGRGGAV
                     HTRASEVNAVLAKGFDAARAANRPFFAFANYMEAHDPYTPDRRFLGRFPGYDPSLPRR
                     AGRFIGAAGSRRVIDGRERRHLVSLYDSAIATLDRELGLLFDRMRTMGLYDGALIVVT
                     ADHGEMLGERGVFGHGAGLVDEVLRIPLIVKFPFQREARRVAIPVSQVDVVPTLLDLL
                     GLPPDPGLDGISLLEAEQAGERELLAESPPERAVFRAGSTVLERVPAGQGPGPLDPEA
                     LERLKSLGYVR"
     gene            17382..>19123
                     /gene="pepF"
                     /locus_tag="PKK95_02740"
     CDS             17382..>19123
                     /gene="pepF"
                     /locus_tag="PKK95_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HOC17153.1"
                     /translation="MIRITLVPLVLTSAAILASFAYLDATAGQERERKDIPERFKWDL
                     TDLYPSEAAWREARERLEARLPRIRQFEGRLGESAARLEEALETMSGIGRELSRLYAY
                     ASQLSDQDTRESKGQAMTQEMTRLAAAFAAEAAFVEPEILRMDRAKIEAFVRERPGLA
                     PYDHFLDDILRRQPHTGTEAEERLIAQAGVLASTPSDIYGILADADFPYPAVTLSDGR
                     TVTLNSSAYNLYRALPNRDDRRLVMSSFFGALGAYRRTFGATMNASVQRDLFYMKARK
                     YNSSLEAALNEHNIPIGVYQSLIDGVNANLATFHRYLGLRKRMLGVDELHYYDLYAPL
                     VGSVDIDYPIEQAQREIVEALAPLGREYTSVLERSFRERWIDLYPNPGKRSGAYSNST
                     YDVHPYMLLNYNNKYADMSTLAHELGHTMHSFLSNRAQPYPKADYPIFLAEVASTFNE
                     ALLIDHVLEKITDRDARLSLLGNYLENIKGTVFRQTQFAEFELRVHEMAERGEAITGD
                     ALSALYADITKRYYGHGAGVTVVDDYVAHEWAFIPHFYRAFYVYQYATSFTASAALAE
                     RALAGDQETIRKYL"
BASE COUNT         2875 a         6763 c         6705 g         2780 t
ORIGIN      
        1 cgcctggcgc gggtgaaggt ggggcagccg ctggagttca ccaccgacgc actgcccggc
       61 cgcacgttca cgggcaaggt gatgttcatc aacccgacga tcgacccggc cagccggtcg
      121 gcgaaggtgg tggccgaggt gcggaacacc gacggccagt tgaagggcgg ggcgttcgtg
      181 aagggccgca tcctggtggc caaccggagc ggggtgctgc aggtgccgcg cgaggcgctg
      241 cagaactgga acgtcgagca ggcaacggcc gacgtgtacg tcgtccgggg agatacggct
      301 gaccggcgcc aggtgcggat tggcggcgcc acggccacct cggtcgagat cctcgacggt
      361 ctgcaggcgg gcgagcaggt ggtgacccgc ggctcgttcg cgctgaagcc gggcgaccgg
      421 attgcccccg cgaagggcca gggggcgtag cgatgttcct ctcgaacctg tcgatcagcc
      481 ggcccgtcgt cgcgacggtg atgatgctga cgctcgtcac cctcggcctc ttctcctgga
      541 ggcgcctgcc gacggacatg atgcccgagg tcgagatccc ggtgctctcg gtcgtcgccg
      601 agctgccggg cgcgtcgccc gagacggtcg agcgcgaggt gagcaagaag atcgaggagg
      661 cgctgaaccc gatgtcgggg gtgaagcacg tcaactcgat ctcgcgcgag agcctcgcga
      721 tgatcatcgt ggagttcgac ctcgaggtcg aggtggacag cgcctcccag gaggcgcgcg
      781 ccaagatcaa cgcgatccgc cgcgatctgc ccgcgaacat gggcgacccg gtcatccaga
      841 agttcgactt ccaggcgatg ccggtgatct cggtggcggt ccggtcgaag acgctgtcgg
      901 cccgcgagct gaccacgctg gccgaccgca gggtcaagcg gcggctggaa ggggtgtcgg
      961 gcgtcgcgcg ggccaagctc atcggcgact cgacgcgcga ggtcgccgtg gacctcgacc
     1021 ccgcccgcct ctcggccctt ggcatgggag tggacgaggt cgtggccggg ctggcctccg
     1081 agaacgtcaa cacgccgctc ggacggctga cgagcgccgg cagcgagatc ccgctgcgga
     1141 tctcggggaa gccggccagc accgacggct acgcgaacat ggtgatcgcg cggcgcggcg
     1201 ggcagccgat ccgcctcgcc gacgtggcga cgatcaccga cacgatcgaa gagccgcgat
     1261 cgctggcgct catcgacggc gagccggcgg tcgcgatcga catcaccaag cagaccaagg
     1321 ccaacaccgt gggggtggtg gacggggtgc tcgccgagct cgaggcgctt cgggccgaga
     1381 tgccggcggg ggtcgagctg caggtggtgc gcgacacctc ggtgttcatc cgcgaggcgg
     1441 tgcacgacgt ccagaacacg ctgatcctcg gcggcctgct gacgatcctc atcgtcttct
     1501 gcttcctgaa ctcgtggcgc tcgacggtca tcaccggcct gacgctcccg atctcggtca
     1561 tctcgtcgtt catcgtcatg tacttcctgg ggatgacgtt gaacatgctg acgctgatgg
     1621 cgctgtcgct cgcgatcggg ctgctgatcg acgacgcgat cgtcgtccgc gagaacatcg
     1681 tccggcacct cgagcgcggg gaggaccact tcgaggccgc gcgcaagggg acggcggaga
     1741 tcggcctggc ggtgctggcc accaccatgt cgatcatcgc cgtgttcgtg ccggtcgcct
     1801 tcatgaaggg ggtcgtcggg cgcttcttct accagttcgg cctgacggtc gccttcgccg
     1861 tgctcgtctc gctgttcgtc tcgttcaccc tcgacccgat gctctcgtcg cgctggatcg
     1921 accccgacat cgagcggaag gggcggcgca acgtcctgca gcgggcgctc gaccgcttca
     1981 acgagtggtt cgagcggatg gccgacgggt acaagcgcat catcgggtgg gccctcgacc
     2041 accggttcgt ggtgtcgggc gtggcggtgg cggcgttcgt ggccgggctg ggagtcttca
     2101 ccctcctgca gaccgagttc atgacgccga tggaccaggg cgagttcgtc gtgaagttca
     2161 agagcgcgcc cggctcctcg atcgccgaga cccgcggacg ggtcgaggaa gtgctcgggg
     2221 cgctgtccga gttcgaggag gttcggtaca cctacgcgtc gatcggcgcc ggcgactccg
     2281 acacggtgcg cgacgcgacg gtgttcgtga agctggtttc gaaggacgag cgcgagcgcg
     2341 gcctcaagga cttcgtgcac gacgcgcggc tgcgcctgca gaaggtcccg gggatcgtcc
     2401 tgtcggtgca ggaggaaacc gacgcgttcc agaagccgct gcaggtggtg ctgcagggcg
     2461 aggacatcgc cacgctcaag cggtacgccg cgcaggtgaa gcacgagctg tacggcatcc
     2521 cggggatcgt cgacatcgag gcgacgatgg agctcgacct gcccgagtac cggctgatcg
     2581 tggaccgcga gcgggcggcc gccacgggcc tcggcagcgc cgccgtcgcc aacacggtgg
     2641 gcgtgctggt gggcggccag gccgtcacca cctacgagga cgaggaggga gagggagtgg
     2701 cggtccgggt gcggctgccg caggacctgc gctcccaggt gtcgcaaatc ggcgatctga
     2761 aggtgacggt gccgtcggcc gaggggatgg cgctcgtgcc gctcagcgac ctggtcacct
     2821 tcacccgggc cacctcgccg gccgagatca accgccgcga cctgtcgcgg caggtcacca
     2881 tcgacgccaa cctcgacaag ctggcgctcg ggaccgccag caacctggcg ctcgccgccg
     2941 ccaacaagat cgagatggcg cccggctaca aggtggtgat cgggggcgac accgagatga
     3001 tggtcgagtc gttcggctac atggccgagg cgctgctgct ggcggtcatc ctcgtctacc
     3061 tgatcctggc ggcccagttc gagtcgttca tggacccgct ggccatcatg ttctcgctcc
     3121 cgctcgcggt ggtcggcatg gcggggacgc tcgtgctgac cggcgacacc atcaacatca
     3181 tgtccctcat cggcctgatc atgctgatgg ggctcgtcac gaagaacgcg atcctgctgg
     3241 tggactacac gaaggtgctg cgatcgcagg ggctcgagcg gcgcgaggcg ctggtgctcg
     3301 ccgggcggac gcggctccgg ccgatcctga tgacgacctc ggcgatgatc ttcggcatgc
     3361 tgccgctgtt cttcgccatc ggcgagggcg ccgagttccg ggcgccgatg gcccgcgcgg
     3421 tcgtcggcgg cctgatcact tcgaccgccc tcacgctgat cgtcgtgccg gtcgtctact
     3481 cgctcctcga cgacgtcacc gcctggctgc tcgggcggaa ccggaagaag aaggtggcgg
     3541 cggtggccac gacggcgctc gttgtgctgg cgctggcgct gacgccctcc gcggcgttgg
     3601 cccaggcgcc ggcagcccag ggctcctcgg cggggacggc gctctcgacg cttcgcgagg
     3661 ggatgctggc ggccggcagg ccgtcggccg ggctggccgg ccaggcggcg tcggccgcgc
     3721 aggcggtgca gcccgaggcg gccatcccgg tcaagacgct cacgctggag caggcgcttg
     3781 ccatcgcggc cgagcagaac cgcgacatcc tgaaggcgcg cgagtaccag cactgggtgc
     3841 aggggcgcta cgagcaggag cgggcggccg ccctcccgcg ggggtcgctc agcctcagca
     3901 cgacgcgcac cttcgacgac tcgatgacca agctcttcaa ggacttcatg cccgaggagg
     3961 gcgtcggcgg cgagaccggc atcggcgaca tcttcggcgg ccgccaggac gtcgggatcg
     4021 ccgggttctc ggtgaaccag gtggtgttca cctggggcca ggtgggcgcg gccatccgcg
     4081 ccgcgaagct cgggttcggc gtcgccgacc agcagcttcg ccgcttccag caggcggtcg
     4141 agaaggacgt caccacggcg ttctacgacg tgctggcagc gcgcgagctg gccaccatcg
     4201 cgcggcagga cctcgagcag cgccagcggc acctcgacga gacgatcaag cggcagtcgg
     4261 cgggcacggc caccgactac gacgtgctcg ccgcgcgggt ggcggtcgag aacgggcggc
     4321 cggcggtcat ccgcgccgag aacggcgtcc ggctggcgcg cgagcagctg cggttcctgc
     4381 tcgccgagcc gggcgaagtg gatgcagccg gcgcgctggc cgcgacgatc gagccgcccc
     4441 cgccgtacga gcaggtgctc gcaagcgccc tcgaaaaccg ccccgaggtg gccgagctgt
     4501 cggggaccaa ggggatctac ggcgagctgg tcaccatcgc gaaggcggcc aacaagccga
     4561 gggtcgactt ctcagcgagc tggggccagc ggcggctcgg tttgaagacc atctcggcgc
     4621 gcggcaccac gtggaacgcc gggctcgtgg ccacaatccc gctcttcgac ggctggcgga
     4681 cgaagggggc ggtggcgcag gcgcagagcg acctggtgcg actcaacttc gacgagttga
     4741 agctgcgcga cgggatccgc ctcgaggtgc ggaacgcgct gaacgccgtg cgcgaggcgt
     4801 cggagatcgt cgcggccctc ggggggacgg tggcccaggc cgagaagctg ctcttcctcg
     4861 ccgagaaggg gttcgagctc ggggtcaaga cccgcctcga ggtgcaggac gcggagctga
     4921 acctgtcgtc agcccgcgcg aacctcgcgc gcgcccagcg cgactacaac gtggcgcgcg
     4981 tcaacctcga gtgggtcgcc gggacgctgg ggcagtagcc gtgatgtaga ggcaaaacgt
     5041 cgaatgtaga aggaccgagg ggaaccggga gcgcgcccgg cgcgcgctcc ccggttcccc
     5101 ttttttgcat tctgcgttct cttcgtcctg ctcttcaggc tactgtccgt cggggccgat
     5161 ggcctccatc agccccatca tctccagcgc cgcgagcgcc gcgtcgaacc ccttgttgcc
     5221 cgccttggtg ccggcccgct cgatcgcctg ctcgatcgtc tccgccgtga tgacgccgaa
     5281 ggtgatgggg acgccggtct cgagcgccgc ctgggccact cccttcgtca cctcggcggc
     5341 gatcagctgg aagtgcgggg tgtcgccctg tatcaggcac ccgaggcaga ccaccgcgcc
     5401 gtagcgcccg gtccgcgcga gacgccgcgc gacgagcggg agctcgaagc tgcccggcac
     5461 ccacgcgacg tccacatcgt cgtcgggcac gccatgtcgc gccaggcagt cgcgcgcgcc
     5521 gcccagcagc tccttggtga agaagtcgtt gaagcggctc accgcgatcg ccacccgctt
     5581 tccgggcgcc tcgaaccgtc cctcgaacac cttgcccatg cgtcgccctc cagttcagcc
     5641 cagcacgtgg ccgcctgccc gaaggccggc accctgtcca caattcttcc tgccggatca
     5701 gcgctccctt tgtgcccttg atccgtcgtg tcctttgtgc cctgtgtggt taatcgcttg
     5761 tgctcttccc tttgtgttcc ttgtgtcctt tgtggttgat ccttcgtggt acgtcacacc
     5821 cgttccagca ggtgtcccag ccgcgccttc ttggtgcgca ggtaggtcag gttgctggcg
     5881 ttcggcggca cctctagtgg tatgcgtcgc acgacgacga ggccgtaccc ctcgagcccc
     5941 acgatcttcc gcgggttgtt ggtgatcagt tccatcggcc ccacccccag gtcgcgcagg
     6001 atctgcgcgc cgagcccgta gtcgcgcagg tcgtccgcga acccgagccg caggttcgcc
     6061 tcgacggtgt cgctgcccag ctcctggagg ccgtacgcct tcagcttgtt gaccaggccg
     6121 atcccccgcc cctcctggcg catgtagagg aagatcccgc tgcggttcgc ctcgatggcg
     6181 tcgagggccg cctccatctg ctcgccgcag tcgcagcgca gcgagtggaa gacgtcgccg
     6241 gtgaggcact cggagtggac ccgcacgagc gtcggagccg caggcgacgg cgtccccttc
     6301 acgagcgcca ggtggatgcc cgaatcgacg gtggtgctgt aggcgacgag cctgaaccgg
     6361 ccgtggcgcg tcggcaggtc cacctccgac tcgcggcgca ccagctgctc gtgaagccgc
     6421 cggtaggcga tcagatcggc aatcgacacc agcgccaggc cgaacctgtc ggcgatctcc
     6481 cggagcctcg gcagccgggc catcgagcca tcctcgtcca tgatttcgca gaggatgccg
     6541 gccgggtaca ggcccgccag gcggcacagg tccacgaccg cctcagtgtg cccgctgcgg
     6601 cgcagcacgc cgccgggctg ggcctcgagg gggaagacgt ggccgggccg cgccaggtcg
     6661 cccggccgcg tggcgggatc gatcaggacc ttcaccgtcc gggcccggtc gaacgcggag
     6721 atgcccgacc cgatgccgat cgcggcgtcg accgaaaccg tgaacgaggt gccgagcgcc
     6781 gcggtgttgc ggtcgcgatc caccatcggg cgcaagtcca gcgcccgcag gcgctccccg
     6841 gtcgtcgaga agcagatcag gccgcggccg tgccgggcca ggaagttgat cgcctctggc
     6901 gtcaccttct cggcggccat gacgaagtcg ccctcgttct cccgatcctc gtcgtccacc
     6961 acgatcacga tctcgccgcg gcgcagccgc tcgagcgcgg cctcgattga atccagcgtc
     7021 tggccactca tctcccgttc ccccgattgc acgtccttca tcggccccac cctcgcgcga
     7081 gcagcgcccc catcgtcagc cgccccggct cgacacgttc ggccgccttc gccagcacgt
     7141 cgagctcgag gttgagccga gcgccgggcg caaggctggc gaaccgggtg gcaccgagcg
     7201 tgtgcgggat cagcgtcacc gtgaaccgat cctcgaacgg cccctcgtcg agcgtcaggc
     7261 tgacgccgtc caccgcgatc gagcccttcg gcagcagccg gcgggcgagc gccctcgcaa
     7321 gcgtgacggt catcgacgcg gcagccccgc gccgttccat gcgctgcagg cggcccgtgc
     7381 catccacgtg gccgagcacg aagtgcccgc ccagggcgtc gcccgcacga agcgccggct
     7441 cgaggtgcac gcggccgccc accctccagt cggccagcgt cgtcacacgc ctcgtctcgg
     7501 cagatgcctc gaccgacagg cgcgtggcgg caacgcgcac gaccgtcagg cagatgccgt
     7561 tgacggcaag gctgtccccc accgcgggcc cctcgggcgg ccgcgcgacg aggggggcct
     7621 cgatctcgac gagcgtcacg ccgccgcgac gttcgaggcg gtgtacccgc ccgatctcgc
     7681 ggacgaggcc cgtgaacatc acgcacgctc ccagtcgttg gccccgccca gcgaggtcag
     7741 ccgcgcgcag gcgtcccgga acgagagccg gtcccagacg gcaacggcat cgccgccgca
     7801 atgcgcggcc ctcgtcaggg tcaggccatc cggctcggac ccggcgccgg gcggctcgtt
     7861 ccattcaggc cagccgacgc cccgtcccag caggacaggg gccgtgtagt ggacccaccg
     7921 atccacgagt cgggcgcgaa ggaaggaggc ggccacggcc ggcccgccct ccacgaggag
     7981 gcagtgtttt cccaggcttg cgaggcgggc caccaccgaa cacggttcga cgccggaggg
     8041 gcccgcggcg cagcggatca cggttccttt cccggctctc acggtttccg cggcgagttc
     8101 cggcgcgtca ggcccggcaa acgcgaggtg ggggcgtccg gcccagggcg gcagatcggc
     8161 cagccgctcg ctcagcaccg cggggatcgg atcctcgggc gggcacgccg catcgggccc
     8221 ggccagacgg ccgtcgaggc gcggacggtc cacggcggcc gtgcgcgagc caacgaggac
     8281 gacatcggcc caccggcgca acacgtgcac ctggtgccgc gcgcttgcgc ccgtcaagta
     8341 ggccgcgccc tttcccggcg tcatcccctc cggggcgaag cggccgtcga gggagacggc
     8401 tgtcttgagc cagacgaacg gccgctcgaa cgcgcgcgtg accacgaacg gccacgccag
     8461 gtcgagcgcc tcctcggcgc agacacccag cgtcacggcg aggcccgcgc agcgcgcaac
     8521 ctcgagtccc cgtccggcga cgtgagggtt gggatcgacg atcgacacga cgagccgtga
     8581 cacccctgcg gcgaccacag caggagcaca cggcggggtg cgaccctggt ggttgcaggg
     8641 ctcgagcgtg cagtagaggg tggcgccctt cgcgcgctct ccagcctcgg cgagggcagc
     8701 cgcctcggca tgcggctcgc cggccccgtt gtgcacgccg cggccgacga cggcgccgtc
     8761 gcgcacgacc agtgcgccga ccggcgggtt gggccacggc ctgcgcgccg gccgcgcggc
     8821 cagcgccagc gcctcgagca tgtagcggcg atcgccggca gttgcagcgt tcatcgggac
     8881 caggggaacg tggccgaccg cgggacgggg agggaggaag acaggcaggc cgcgacagct
     8941 ggccgtccag agaagggaac tgccgtctgg agacacagcc ttcgcggaga acggttgcca
     9001 gccccaactc ctcccatcca gactgtcact gtcggccccg gaatcgcacc ggatcgtgcc
     9061 cttgcgggct cgcgggctct accgccggtg gggacttgca ccccgccccg aagttgtggc
     9121 ggtttcaacg taacggttcg cgggtcagag tgtcaaggag ggaagctggg caagggtaag
     9181 gggtcgggag taattagaag ccggaaaccc ggcatgactt ctaattactc ccgaccccgt
     9241 tcggctactt cttcaccagg ctcggccagg cgagccaggc gccgatcgcg atgaaggtga
     9301 ccggccacca gtcctcgagc cagtcgagcg gcatgttgaa cagcgtgttc atcagcagca
     9361 cggcgcccac caccaccagc gccaccccgc cggccagcga cccgcgcccg cccttcacca
     9421 gcgcgaagtc gtcgggaatg tccgccgcgc cgatgccggc cagcgcgttg ttgtagaaca
     9481 cggcgcggcg ccaggcatcc acgatgttgt agagccagta gaaggcgagg aacagcccaa
     9541 agaacggctc tgcgcctccg ttgacggcgc cgctgttcag gagcccgatc aggccggcga
     9601 tgatcagcgc gttggtgaag ccctgctggt agtacccgac gtagacctgg ccgaggcctg
     9661 gcattgccga cagcaccagc gcgagcatcg gcgacttgcg ccgcgggtcg tcgtagtagt
     9721 cgcggcgagc cggtgccggc gacggccgcg ccgccgcctc gggcgccctg gcgcccgcgt
     9781 tcacgtcctg gaccggcgct ccgaggtttt cctggtaggt gttcatgatc gtctctccgt
     9841 tatcttcttc cgctgccgtc cgtcgccttc gctctccctg tctcggtaac gcggccggag
     9901 gtcgaagggt tcggtcggcg cagctccgac acccaggcca cgagactcgt gaaggcgtcg
     9961 cgcagcgggc cgaacacgcg agtactgacc cactcccacg ccggcgacac cagtgcgaag
    10021 gcccgcgagg cgaccccggt ttcgccgcgc cgcgccgacg ccgcccgccc agcgaccgct
    10081 tccccgattt cccgaacgag gccggccggt cccgacgaca cccgctccag cacctccacg
    10141 acgcgcggct ccgccagctc gcggccccgg gccgaggttt cgcgaaacgc ggccacgggg
    10201 ttgccgacga tgatgaagat cagcagcgtg cacaggtagg cggcctcgag gctgaagcgc
    10261 gggcgtgcgg ccagtttcga gagccaggcg gcgacccgtt cgtcgaaggt gggcgcgggc
    10321 gggcgccggc tggtggccgc gatcacggcg ggtgcgaggt ccgccggcgg ctgcagcgcc
    10381 gcgaaggccg gcagcagctc gctggcccgt gccatcgcgg ctgccagctc ggcgcagggc
    10441 cggcagcgcg ccaggtgccc ttccaccagc tcgcggtcga ttccttccag gtcgccgtcc
    10501 accaggtcgc ccagccgctc gcgggccgcg ccgcaggtgg ccccgctcgt ccgcgccagc
    10561 acctcgtcgg ccagcgactc ggccccggcc gcgtcctgcg agccgccaag gcccgccatc
    10621 gcgtcgagca gcaggcggca gcgcgcgcat gccgccgcgt gcgcctcggc ctcccgccac
    10681 tcggcggcgc tgcacgaccc ctcgagcagc gcgtcgagcc gccgatcgaa tcgtgcgcag
    10741 tccatctcgc ccctcatcgc gcgggccccg cctcgcgcct cctcagcgac agcacctgct
    10801 cggccagctc gaggcgggcc cggttcgagc gcgacttcac ggtcccaatc gggatgccga
    10861 gcgtcgcggc gatctcggga aaggagagcc cctggatttc cttgaggaga atcatctcgc
    10921 ggctcagctt cccgagcgac ctcagcgccc gccacaccag ccggcgctcg ttcagcgagg
    10981 ccagctcctg ttccggcgtg ccgccgcctc ccgccaggtc caccatctcg tcggccagca
    11041 ggtcctcggc cggcggcctg gccttcttcc tcctgagctg atccaggcag aggttgcgcg
    11101 caacccgcac gagccagggc acgaagacgt cggatggcgc ccaccgcccc cgcgtctcgt
    11161 agagccggat gaagacgtcc tgggccaggt cgcgcgcctc gtccggaccc gaggcgtaag
    11221 cggacgtaat cgagtagatc cgcccctgga actggcggac gaacgcctcc caggcgaggt
    11281 tgtcgccttg gcggcaccgg tcgatgagcg cggccaggtc cattaggtcg atccccgggg
    11341 gacgctccat ggcgcccccg ctacagggat aacgcgcgcg aaggggaaac ggttcgcggc
    11401 ggcgatgtta ccatcccgca tggtttcgat ccgccttcgt cacgccaccg ccctcgtccc
    11461 gatcctcgcc gcctgcgcgc tggccgctcc gggcgccgcg tgggcccagg ccccgcggca
    11521 gctcacggcc gccgactacc agcgggcgca gcgcctgctc gacgcgaacg tgaccccgct
    11581 cgtgctgggg cccgcggtgc gtccggcgtg gctgcctggc gaccgcttca cctaccgcac
    11641 cacggtcgag cgagggaccg agttcgtgct ggtggacgtg gcccgccgca cgcgggggcc
    11701 cgccttcgac cacgccgcga tcgcggcggc cctttcgaga gcgaccggcg cgggccacga
    11761 cccctaccgc ctgccgttcc ggacgttcac cttcgttcgc gacggcgcgg ccatcagttt
    11821 cagggcggcc gggaaggtgt gggagtgcga cgtcgcgggc aaggcctgca gggcggcaga
    11881 gcccgccgcg gccgcgcgcg caggcgggca gcgcatccag cccgtgccgc aggagtcggt
    11941 gtcgccggac gggcgctcgg ccgtcttcat ccggaacgac aacctgtggc tcagggaggt
    12001 cgcgaccggc gcggagcggg cgctcacctc cgacggggtg aaggacttcg gctacgcaac
    12061 cgacaatgcc ggctggacgc gcagcgatcg gcccgtcgtc ttgtggtcgc ctgactcgaa
    12121 gaagatagcc accttccagc aggaccagcg ccaggtcggc gagatgtacc tggtgtccac
    12181 gaaggtggga catccggagc tcgaggcctg gaagtacccg ctccccggtg acgcgcacgt
    12241 gccgatgatc caccgcgtcg tcatccacgt ggacgggccg cgggtggtgc ggctgaaggt
    12301 gccgcccgac ccgcaccgcg gcaccgtcac cgacgacatc aaggacgagg aagggcgcct
    12361 ggacgacgtc aggtgggctg ccgactcgag cgccctcgcc ttcgtctcct cctcccgcga
    12421 ccacaagcag gcgacgctgc gcgtggcgga tccggagacc ggcgaggtac gcgacgtgct
    12481 cgaggagaag gtggacacgt tcttcgagtc ggggaacgac aggtcgaact ggcacttcct
    12541 gcctgactcc ggcgaggcga tctggttctc gcagcgggac gactggggcc acctctacct
    12601 gtacgatctc gttagcggaa agccgaagcg tcagatcacc tccggcccct ggaacgtggc
    12661 gcaggtgctg aaggtggacg agaaggcgcg ggcgctgtac ttcgtgggcg tcggccgcga
    12721 gaaagggcgc gacccctact accggcactt ctaccgggtg ggattcgacg ggggcgacgt
    12781 cacgctgctc acgcccgagg acgccgacca ccaggtttcg ctctcgacct cgggcggctt
    12841 cttcgtcgat gcctactcga cgcccgtgac gccgccggtg tcggtggtgc gcgacctctc
    12901 gggcaaggtc gtgatgccgc tcgagaaggc cgacaccacc cgcctgcagg cgatcggctg
    12961 gaagcctccc atccccttca cggtcaaggt ccgcgacgga atgaccgatc tctacggcct
    13021 gatgttccgg cccaccgatt tcgacgaggc gaagcgctac cccgtcatca acaacatcta
    13081 cccggggccg caggccggga gcattcgcag ccgcggcttc ctgcccgcgc acggcgacgc
    13141 gcaggccctc gccgagcttg gcttcatcgt cgtgcagatc gacgcgatgg gaacgccgtg
    13201 gcggtcgaag tcgttccacg cggcctatta cggcgacatg ggcgacaacg ggctgcccga
    13261 ccaggtggcg gggatgaagg agctggcagc gcggcacccg tggattgaca tcgatcgcgc
    13321 cggcatctgg gggcactcgg gcggcgggtt cgcgagcgcg gccgccatgt tccactaccc
    13381 cgacttcttc aaggtaggcg tgtcgcaggc cggcaaccac gacaaccggg gctacgagga
    13441 cgactgggcg gagaagtggc aggggctgct ggtcgcaaag ccggacgggt cgtcgaacta
    13501 cgacggccag gcgaaccaga acttcgcgaa gaacctgaag ggcaagctgc tcctcgcgca
    13561 cggcaccatg gacaacaacg tcccgccgta cctgacgctg ctggtggtgg acgagctgat
    13621 caaggcgaac aaggatttcg acctgatcat gctgccgaac cgcggccacg ccttcggcaa
    13681 cgagccgtac atgatgcggc ggcgctggga ccatttcgtg aagcacctga tgggagccga
    13741 accgccgcgg ccgatcgagt tcccggcccc gttcagaccc tgatctgaac ccacttcttc
    13801 tggccgaagc ggacgaccga cagggtggcg cgggtcacgt ggccgaggac gatcgccgtc
    13861 cagatgccgc gcgaggtgag gttgccggac gactgcagca gggcgcaggt gccgaggggc
    13921 acgacgatct gcgaaacgat ggtgatgtag agcgggctcc gcgtgtcgcc cgtgccctgc
    13981 agcccgccgg tgtaggtcag cgcgacggtg atgaagaacc ccgagaaggc cagatagccc
    14041 agcaattcgc tgcccagccg ggtgaccgcg gggtcggtga gcccgaaggc ccagagcagc
    14101 ttctcgggga tcagcacgaa cagcgtgccc acgccagccg cgatcagcag gccgccgaag
    14161 gccgccttgc gaacggcctc gacgctccgc tcggggcgtc ccgcgcccag gttctgcccg
    14221 gcggcagccg cggcggcccc catcagcccc accgacgtcc acgtgatcat cgaaaacagc
    14281 tcggtgtacc cgaccgcgta ggccgcctgc gccgccgcgc tctcgggcag cgacccgatg
    14341 aaacgcagca ggaggaggcc ggcgacgttc atggccacgc cctggatccc ggccggcagg
    14401 ccgaagcgga acagcgagcg gatcatcgtc cagtccggcc gccggtcgcc cttcgggaac
    14461 ccgaccacga gccgctcggt gagcagcatt ctgatcgcga cgatgccgac gagcgtggtg
    14521 gcgagcaccg tgccgatcgc ggcgccgagg gtgcccatcg cggggatcgg tcccgccccg
    14581 cggatgaaga tcacgttgaa gaccacgttg aggacggtga tggcgacccc gaacttcaag
    14641 ggcgtgcgcg cgtcgcccgc cgaacgcagc gcggccccga gcatgtagaa gagcaggagg
    14701 ccgaagccga agacgaacag cgtccggatg tatggcaggg cctgcgcgcg cacctcgggc
    14761 gcggcattca ccaggccgag cagccacggc gacagcacgt agcccaaagg cgcgagcacg
    14821 agcagggcca gcgcaaaggc gacgaggaac gcctggtaga ccgcgcggtt caccttctcg
    14881 ggctcgttcg ccccggcgta gcgtgcgacc agcaccgcca tgccggtgaa gagcgagccc
    14941 atgaagacga tgatgaccag gaagatctgc cagctgacgc cgattgcggc gttggcggtg
    15001 tagccgacga agttcccgac catcgcatgg tcgacgatgc cctggaggcc gcccatggcg
    15061 ttctgcagga tcgtcggcca cgacaggtgc cagacggcgc gcccgatggg gccgtcgacg
    15121 atggaacggt cgtagcggcc gaggcgggcc gaaggagggg ccggcacgtc ctgtgcgggc
    15181 ggagccgcgt ccgctggcgg cgctacggga gcgggggcgc gtgtctggtt gtcggcgggc
    15241 accgtttacg atcttagcct gaccggcgcc tccatcattg ataatgtgac cgcgaccccg
    15301 ccgctgagcg gcctgtcgcg gcttgccatc cggagccgcg agcgaagcag cgagctgtcg
    15361 cggcttgcga catgacagac ggctcgaggc ccacgacatc ggcggttgcc ttcgcggcgc
    15421 aggggggcct ggtctggatg accttcgggc tcctcgagca cttgtgcctc tcggtactgc
    15481 ccgcgtggcg cgagtcgtcg ctgctgctgg tcccttcgtc gcgggccgcc gcgctcgcgg
    15541 tgacgctcgc cggctacgcg gttgttggcg ccctgctcgg cggcgccgcc ggtgcgggtg
    15601 cgcggttgct cggctggggg ggcggcgacg ccccgtccgg ccgaagggcg gccgcggacg
    15661 ccgccacggc gacgctggtg ctcgcgtgga ccgtgcacct tgccgcggca cgcagcttca
    15721 gccgctcggc ctacctgggc ctggccgtct cggcgctgct cgtggcggcg ctcgttgccc
    15781 ggacgggctg gcctcacggg cgcgtgccgc gcgtcaccgc ctggctggtg tccccggccg
    15841 gggcgtcggt ctgcctgctc gcgccgctgg tgttccgcga ccggctgcat ctcgcggcgc
    15901 tcggcgagca cctcggaaca tcggcaggcg tcttcaaggc cggggccgcg gcggctggca
    15961 cctcgctgct cgtcctgctg ctcgtcgcgg gggcgctgtt ccaccgtgga accgggcggc
    16021 acacggggcg cgcgaggctc gcggtcttgt tggcgctcgc cctcaccctg ggtgcctcga
    16081 gcgtgctgct cgatcgcgcg gcgttgtcgc gcttgagcgc cggcggcgcg gcgcccgcgc
    16141 cacgggaatc caccgcccct ccccggccca ccgggatcct cctgctgacg ctcgacgcgg
    16201 tccgcgccga tcacctgccg gtctacggct acccgctcga cacgtcaccg aacctcgcgt
    16261 cgctggcccg ccacgcgacc gtgtacctga acgcgcgggc ggcgtccgac atgtcgctcg
    16321 ggactcacgc ctcgctgttc accgggaagt accccagccg gcacggcgcc tgggtcggcg
    16381 gggcgttccc cgtgggacgg ccgctggccg cctcggagcg gacgctcgcc gaacgagcgg
    16441 ccgaggcggg ctgcatccgg ttcgcggccg tggcgaatgg gtacttcctg aataccgcgt
    16501 acggcctctc gcgggggttc cagctgttcg acgttccgcg ggccgatcgt ctcgagctga
    16561 ggctgcggct cgacttcgcg gcccgccgcc tcgcgggccg cggcggtgcg gttcacacgc
    16621 gggcgagcga ggtcaacgcg gtgctcgcga agggcttcga cgcggcgcgg gcggcgaacc
    16681 gtcccttttt cgcgttcgcg aactacatgg aggcccacga cccgtatacg cccgaccggc
    16741 gcttcctggg ccggttcccc gggtacgacc cgtcgctccc gcgccgcgcc gggcgtttca
    16801 tcggcgcagc ggggagccgt cgcgtgatcg acgggcgcga gcgccgccac cttgtctccc
    16861 tctacgactc ggccatcgcc acgctcgacc gcgagctggg cctgctgttc gaccggatgc
    16921 gaaccatggg cctctacgac ggggcgctga tcgttgtgac ggcggatcac ggcgagatgc
    16981 tgggcgagcg aggcgtcttc ggccacggcg ccggcctggt ggacgaggtg cttcgcatcc
    17041 cgctcatcgt caagttccca ttccagcgtg aggcaaggcg ggtcgcgatc ccggtcagcc
    17101 aggtggacgt cgtccccacc ttgctcgacc tgctcggctt gccgcccgat cccggcctcg
    17161 acggcatcag cctcctggag gcggagcagg cgggcgagcg cgagttgctg gccgagtcgc
    17221 cgccggaacg cgccgtgttc cgtgcgggct ccacggtgct cgagcgcgtg ccggcgggcc
    17281 aggggccggg gccgctcgac ccggaggccc tcgagaggct gaaatccctc gggtacgtgc
    17341 ggtagtctgg tagaaccacc tggaaaagga gaatcctgcc tgtgatccgc atcaccctcg
    17401 tgccgctcgt cctgacctcc gccgccatcc tggccagctt cgcgtacctc gacgcgacgg
    17461 cggggcagga gcgcgagcgc aaggacatcc ccgagcggtt caagtgggac ctgaccgacc
    17521 tctacccgag cgaggccgcc tggcgggagg cgcgcgagcg gctcgaggcg cggctgcccc
    17581 ggattcggca gttcgagggc cggctcggcg agtcggcggc ccggctcgaa gaggccctcg
    17641 agacgatgtc ggggatcgga cgcgagctga gccggctcta cgcgtacgcc agccagctct
    17701 ccgaccagga cacgcgcgag tcgaaggggc aggcgatgac gcaggagatg acgcggctgg
    17761 ccgccgcctt cgccgccgag gccgccttcg tcgagcccga gatcctgcgg atggaccggg
    17821 cgaagatcga ggcgttcgtc cgcgagcgcc cggggctggc gccctacgat cacttcctcg
    17881 acgacatcct gcgccgccag ccgcacaccg ggaccgaggc cgaagagcgg ctcatcgcgc
    17941 aggcgggcgt gctggcgtcc acgccgtccg acatctacgg aatcctggcg gacgccgact
    18001 tcccctatcc cgctgtcacc ctgagcgacg gcaggacggt gacgctcaac tcgtcggcct
    18061 acaacctcta ccgcgcgctc cccaaccgcg acgaccgccg cctggtgatg tcgtcgttct
    18121 tcggagcgct cggcgcctac cggcgcacct tcggcgcgac gatgaacgcg agcgtgcagc
    18181 gcgatctctt ctacatgaag gcgcgcaagt acaactcgtc gctcgaggcg gccctcaacg
    18241 agcacaacat cccgatcggc gtctaccaga gcctcatcga cggcgtgaac gcgaacctgg
    18301 cgaccttcca ccgttacctc gggctgcgga agcgcatgtt gggcgtcgac gagctgcact
    18361 actacgactt gtacgccccg ctcgtcggct cggtggacat cgactatccg atcgagcagg
    18421 cgcagcgcga gatcgtggag gcgctcgccc cgctcggccg cgagtacacc tccgtcctcg
    18481 agcgctcctt cagggagcgc tggatcgatc tctatcccaa ccccgggaag cggtcgggcg
    18541 cgtactcgaa ctcgacctac gacgtgcacc cgtacatgct gctgaattac aacaacaagt
    18601 acgccgacat gagcacgctg gcccacgagc ttggccacac gatgcacagc ttcctgtcga
    18661 accgggcgca gccctacccc aaggccgact acccgatctt cctcgccgag gtggcctcca
    18721 ccttcaacga ggcgctgctg atcgaccacg tcctcgaaaa gatcaccgac cgcgacgccc
    18781 ggctgtcgct gctcggcaac tacctcgaga acatcaaggg caccgtcttc cgccagacgc
    18841 agttcgccga gttcgagctg cgggtgcacg agatggcgga gcggggcgag gcgatcacgg
    18901 gcgacgcgct ctcggccctc tacgccgaca tcaccaagcg gtactatggc cacggcgcgg
    18961 gcgtcaccgt ggtggacgac tacgtggccc acgagtgggc cttcattccc cacttctacc
    19021 gcgccttcta cgtctaccag tacgccacct cgttcaccgc ctcggccgcg ctggccgagc
    19081 gggccctggc gggcgaccag gagacgattc gcaagtacct cga
//
LOCUS       DAOPQX010000027        18643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3592_length_18643_cov_4.841295, whole genome shotgun sequence.
ACCESSION   DAOPQX010000027 DAOPQX010000000
VERSION     DAOPQX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 18643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18643
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_18643_cov_4.841295"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKK95_02745"
     CDS             complement(<1..239)
                     /locus_tag="PKK95_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOC17154.1"
                     /translation="MTSTTARPVAAPNLPAPVGPYSPAIVLDRLVFVSGQGAVDPATG
                     RMVGPDVAAQTEQVFRNIQAILEAAGTDLSRVLRCG"
     gene            complement(249..1616)
                     /locus_tag="PKK95_02750"
     CDS             complement(249..1616)
                     /locus_tag="PKK95_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOC17155.1"
                     /translation="MRGFLLLRALAVVVCVLGAAQAASAQGFGVYEQSPCQLGRGGAG
                     VASPCPDASSVYFNPAGLSFEATQIGLGGTVIGPRGDFTDDTTGKVSSLNKKWYPLPN
                     VYASTPIGDRFAVGIGIFAPYGLTTDWPEESQGRFLGYKSVVQGVYIQPTASYKVNDR
                     LSVGAGIDLVRLHVQLRQRLDLSAQALPAVPGLPPGATFGTLGVRAGTDFADINLEGD
                     AWSVGAHFGVLYKASEKVSLGARFMTGQSVEIEEGTIETRQIPTPFVLPVAIPGVAPR
                     GTKLDLLLASRFQPGQLLGPQGAQSEIPLPAQLVAGIAYQATPALKLLADYQFVNWSA
                     FDELPVNGDFLQSSVPENYNDTHGVRAGAEYALSEATTLRAGVDFHTAAAPDETVTPN
                     LPEGVRREFSVGIGQRLSDNLRVDAFYLYLSQPERAGRTNPTTNNGVYSFKANLFGVS
                     FAFGF"
     gene            complement(1753..2580)
                     /locus_tag="PKK95_02755"
     CDS             complement(1753..2580)
                     /locus_tag="PKK95_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17156.1"
                     /translation="MCLATGLQAKGAPRKPADEGAGASARVMRLDDLRRVPRHRLPTI
                     RTYRWAAAAALSVVERELRLPLRGEIVLFSSAEAFRDELAGDGRHAPLPPASSLPTMV
                     GVSTADRICLNLCRLHPHDWPVRIAALAHEMAHVAQYQLAGGRRMGTEQWLREGMAEW
                     VGARVLEVLGEETVASRVEAVRGHLRQASCRSALPRLSDLVSADSWVQLVGEDLEDPM
                     YDVSLVAAAELIDRHGFGALVDYFERFASLDDRRANFRAVFGEDPRVFEERFDRSWR"
     gene            2852..3115
                     /locus_tag="PKK95_02760"
     CDS             2852..3115
                     /locus_tag="PKK95_02760"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOC17157.1"
                     /translation="MTRRQVLRALFASSIAAAGAAPALAGQDKKPAEKVKDPVCGMMI
                     DAASAKGKSEHEGKTYYFCSDSCKKKFDADPKKYVQPPPPPKR"
     gene            complement(3171..4433)
                     /gene="glyA"
                     /locus_tag="PKK95_02765"
     CDS             complement(3171..4433)
                     /gene="glyA"
                     /locus_tag="PKK95_02765"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOC17158.1"
                     /translation="MLDPHLLQADPEIAALLAAEERREFDKIRLIPSENYVSRAVLEA
                     TGSVFTNKYSEGYAGKRYYEGQQLVDPLERLAIERVRSLFGADHANVQPYSGSPANLA
                     VYFALLKPGDTVMGMALPHGGHLTHGWSVSITGAFFRSVQYGVDIATGLIDHGQVREL
                     ALKERPKLIIAGATAYPRIIDFEAFRRIADEVGAHLLVDMAHIAGLVAGGAHPSPIPH
                     AHVVSTTTHKSLRGPRGAMLLCKEEMAKAIDKAVFPGLQGGPHNHTTAGIAVALKEAA
                     TPEFSAYAHQIVKNARALAEALAGYGFKLISGGTDNHLILMDVTPRGMTGKAYARALD
                     RAGLECNYNTVPGDPRKPFDPSGLRLGTPSVTSRGMKEAEMPRIAGWFNRVAEHVADE
                     AELDRIAGEVREFCAGFPCPGIARPVSA"
     gene            complement(4456..8235)
                     /locus_tag="PKK95_02770"
     CDS             complement(4456..8235)
                     /locus_tag="PKK95_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC17159.1"
                     /translation="MARVKLEIDGRRVSADAGETILDAARSVGISIPTLCHDRQLEPF
                     ASCYLCVVKVKGARNLLPACSTRVAHGMVVETNGLEVRQSRKAALELLLSDHYADCVG
                     PCQLACPAGIDIQGYIALAAIGKYREAIALVKEHNPLPSVCGRVCTRPCEVKGCRRTL
                     LDEAVGIDYIKRYIADLDLAGASPWRAEPAPVTGKRIAVVGAGPAGLSAAYYLALRGH
                     AVAIFEAQPEPGGMLRYGIPEYRLPKQVLDLEIAQILALGATLHPNQALGRDFTIASL
                     KDEGFDAIFLGIGAWRSSLMRVENEESPGVVAGIDFLRDVGLGRPMALQGTVAVVGGG
                     NTAIDCARTALRLGASTVKLLYRRTRAEMPANDSEIQDAIEEGVQIEYLVAPTRVVPG
                     ADLRLRALECQRMELGAPDASGRRSPRPVRGSEHEVPCDLVIAAIGQSATIADLLGHV
                     PGLLPAGESLGLTRWQTVEVDPKTLETTVEGVFSGGDVVTGAATAIEAIAAGRKAAYG
                     IDTYVREGRARPEPEPFLSRKDVFAKITIEDLRSRESKPRRVMPLVPVEERVKGFVEV
                     ELGYTGDDLHEEAARCLECGCVALFDCDLRKYATEYGVEVSRFLGEARQHERDLSHPL
                     IELDRNKCILCGRCVRMCSDVVGVSVFGFVNRGFGTIVAPALGGSLLDAGCISCGLCI
                     GTCPTGAIAERLTLEKPGPWKTDRTASVCSYCSVGCRLSFESLGDTLVSATRREDDHV
                     VFGTHCWQGRFGFHHLGGPGRLTAGQVRENGAAVQAGVDEAIAAAADRLTRALATCRP
                     DEIAVFVSPRMTNEEAYLAQKLARGILGTHQVASFGALVNQAYFAPEAIASATYQDLA
                     AADALVVAGARLAEERFTLHLAAARAARGGASIVWIGPEPDRTADLADVCLQCRPGAE
                     PLVAAGIAAAALRRAGEDPVSHPDLGMVASLGPSQWLEWTGVKAEAIERAAERVRAAS
                     RRVFVFDADYRGERRTGDARAYAAAARALGCALLPAREGANAQGLLDVGAHPAWLPGY
                     RPRHGGAAGDLERLWGVPLVALSSPAPDLAWQLADRRIKVALAFGIDPLGASGIPDAL
                     RAGLAAADLLIAGDVFPTATTRAAHVVLPMSSSVETSGTMTNAERRVQRLRRVIPPPS
                     GLETWQVLCALGRALGGRGFDHASPDEIFEEIRRAAPIYRQVTVDAEGPEGLWDAGIF
                     PLPPVPAVSSASAPSRPVPTSGLDATSARFAAWFEKRVPRPALTSLRAENDDGGV"
     gene            complement(8290..10053)
                     /locus_tag="PKK95_02775"
     CDS             complement(8290..10053)
                     /locus_tag="PKK95_02775"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOC17160.1"
                     /translation="MTRLTVGLGTCGIAAGAEETFRSLEAKAAGRPGLQVVRTGCIGM
                     CYQEPLVEVRTRDGARHLYGRVTPDRLDRVLDEHVGQGRPVAEWLVWTDRGGGSDQAF
                     LARQHRIVLRNCGTIDPESIDEYLAAGGYEALERVLAGIEPDALIATITESGLRGRGG
                     AGFSTGVKWRYARQAPGERKFIVCNADEGDPGAFMDRSVLESDPHSVLEGMILAGYAV
                     GADHGYIYVRAEYPKAIERLTIAIRQAEERGLLGGSILGSRHSFHVTLKEGAGAFVCG
                     EETALIASIEGRRGMPRVRPPFPAVKGLWGAPTSINNVETLANIPWIVLNGARAFAAL
                     GTEKSKGTKVFAMAGRIRHGGLVEVPMGITIREIVHDICGGIRDGRKFKAVQMGGPSG
                     GCISSELEATLIDYESITRTGAIMGSGGLVVMDDTTCMVDVARFFLEFTQRESCGKCT
                     FCRLGTKRMLEILTRITEGDGKESDIPLLEELAERVRQSSLCGLGQTAPNPVLTTLKY
                     FRSEYEAHIVNRKCPAHHCQKLLTYTITDDCSGCMLCLKACPAHAISGERKKVHVIDQ
                     ALCTVCGECYKVCNLGAVHRE"
     gene            complement(10050..10520)
                     /gene="nuoE"
                     /locus_tag="PKK95_02780"
     CDS             complement(10050..10520)
                     /gene="nuoE"
                     /locus_tag="PKK95_02780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOC17161.1"
                     /translation="MQQHRRYDLRNHHYEGEEGALIPLLQRAQQEDGYLRRERLVEIH
                     RRTGIPLAHIYGVATFYAQFRLKPVGKHLLRVCHGTACHVSGAVELSQAIEKHLGAPT
                     GETTADGQYSIETVSCLGCCSLAPVMMIDQATYGNLSPTNAVKVLKKRQKGERP"
     gene            complement(10654..13488)
                     /locus_tag="PKK95_02785"
     CDS             complement(10654..13488)
                     /locus_tag="PKK95_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOC17162.1"
                     /translation="MEFPRAGRYRDDVFVGSRRGRRAWVAIAALALLLFLGAGTLISW
                     YVEELWFDSLGYGDVFWTTITYKTALFWGVALATFLLLFGVFRLVRPRNLAQSTFYVN
                     NQPVRFSLEPVVNIVSWVLAALVALVAASTVVQRWDEVALYLHAPAASGLFSTVDPIL
                     GRPIPFYLFTLPVLHLAFGWLTAVAVVALLASLGAFVVSGEASRTMLGLPNGRVGYRD
                     VSLTLGVLLLVMAGRVYLSRFERLFDDHTVFSGVGYTEATILIPGMLWVSAALVLGGV
                     VAIVNGLRFRRAAGLAAALAPAVLVYVVLSITSWYVAGFVVKPNQLVRERPYISNNIR
                     FTRMAFALDQMEERSFPAETSVDALDLAGNRETLDNIRLWDWRPLQDTLRQIQEIRTY
                     YDFPDIDLDRYRVGGQVRQMMVTAREMNLEKLPESSRNWINEKLIYTHGYGVTMNTVN
                     GFTPEGLPELVLQDMPVQSRLPEITVTRPEIYFGEITNTDVYVRTRQREFNYPQGETN
                     AYTTYDGKGGIPIHSLTRRLLLAFNRGDLSKLPFSDDVTNESRLLMRRNIRERARAVA
                     PFLIYDSDPYIVVGDDGRLYWMMDGFTSSAQFPYARRYRLGGRPLNYLRNSVKVVVDA
                     YDGTMTYYVFEPDDPIIAAYRGLFPTLFADASAMPDHLRAHVRYPELLLEVQAAAYSL
                     YHMEDPDVFYNREDLWSVPSEVVSGEGRQQAARPVEPNFIVMRLPGESALEFVEILPF
                     TPANRNNMIAWMAGRSDGEHYGRVRVYNFSKSRLVDGPLQVEARIDQSPELSEQLTLW
                     SRQGSNVRRGSLLVIPIGRALLYAKPIYLQAERSPMPQLRLVVLALQDRIGYGSSLEA
                     AMTSLFGAGTKAPPVAEPAATAEPGDAAPPATPPSVQALIDRAARELAEYQRLTAEGR
                     LGEAGQRLEALKGLLEQLQKRSAPQAPVNR"
     gene            13605..14705
                     /gene="dinB"
                     /locus_tag="PKK95_02790"
     CDS             13605..14705
                     /gene="dinB"
                     /locus_tag="PKK95_02790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOC17163.1"
                     /translation="MGPVRRILHVDMDAFYASVEQRDRPELRGRPVVVGGRPDERGVV
                     AAASYEARKFGIHSALPMARAVRLCPGVVIVHPDFAKYRAVSGQVFEIFRSVTPLVEP
                     LSLDEAYLDVTSNAWGEPLGVDVARRIKALIFERTGLTASAGVAPNKFLAKIASGWKK
                     PDGLTVIGPSRVESFLQGLTVDALWGVGPVTARRLHDAGIRTVMDVRAAGETRLHEAV
                     GSLAGWLVQLARGEDDREVEPERPAKSSGTENTYAQDLRDIDRIRAEVTSMAREGAAW
                     LLRHELYARTVTIKVRYGDFQTVTRSHSARPATRDADAIAARAVELLARTEAGKRPVR
                     LLGVSVHNFSETPGEAPPARRRPLPLFDPFDG"
     gene            14722..16569
                     /locus_tag="PKK95_02795"
     CDS             14722..16569
                     /locus_tag="PKK95_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17164.1"
                     /translation="MDQTRAAAYREAAAANRRERDGWSRRSRRIAVARVAVFLSASAL
                     TLWALQGRGEWSAPALATGALGFLGFFALVVWHSRVESAERRASAFAQTNEEAALRIE
                     RQWSALPPPRPSPADAAHPYADDLDLFGRASLFSLLGSAGTEAGRQALARWLLHPARP
                     EEIRERQQAVRELAGREAWREALAATARMVEANPHELDVFLEWATSSPWLTPKRWLLW
                     TARAATATTLPLAALHASGAIGSPYWLAPLLVSLILMAVYERRIHQTFSQAFSRERLF
                     HEHAALFEHLAGAEYAAPLLRGLADRLQASGLTAPREMAALGRVMRLADLRFQALFHF
                     PINALTLWDVHVLAALERWQARAGVHLPRWFAALGEFEALSALAWLAHDHPAWAFPEV
                     HESANRLEGVDLGHPLLPDGRRVGNDVTVGPPGQCLLVTGSNMSGKSTLLRAIGVNVV
                     LAQAGGPVCARRLAMPPLSPFTSMRVQDSLEEGVSYFMAALRRLKLLVSEAERVPGDG
                     PMLLYLLDEILQGTNTAERQVAVRRIMAHLLELPAIGAITTHDLELASAPELAGRCRA
                     VHFSEGVSAAGEGVRLSFDYTLRPGVATSRNALKLLQLVGLGEPGTRET"
     gene            complement(16593..17840)
                     /locus_tag="PKK95_02800"
     CDS             complement(16593..17840)
                     /locus_tag="PKK95_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17165.1"
                     /translation="MRTLAAIGAALALTIVVAALDAAPASQPRFPRIYRELGIALLDE
                     WVTDVDLSADGTRLAYARRDPRDWYLDVWTTRPSGREKACITCELPTPTKHRGAPIWH
                     PDGEWLFFSAENDDVRTRKADRMAEPATALNTNLWAVAVGGARAFPLTTFATDYKDPR
                     GVLAPIVAPDGKQIAWAGPVDADPVENVADKTVKPDQESEEANAKGYEWGRWAIYLAD
                     LEIADSTPTLRNIRVLAPAPHDGFYQVDDWSKDGRRLLITASPAGGMGLESLDIYEYD
                     LAAERLRPLTQTADWDHHAHYSADGRQVVWSSSRDLNVKFRSVDGLNWRRDVRTELWV
                     MNRDGSEPRRLTFFNERGHRDHAWFRSRVFRTPRVFVSDNACLLDGTRVAAVLGYEAS
                     AGQLGGVLAVLELNRAAAAKNGE"
     gene            complement(18145..>18643)
                     /locus_tag="PKK95_02805"
     CDS             complement(18145..>18643)
                     /locus_tag="PKK95_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOC17166.1"
                     /translation="EGGAEGVIVETMSAIEEAVAAVKAARAAGAPLVAASMAFDELPN
                     GSVRTMMGVSPEQAARELAAAGADILGANCGTHMQPASFVTVVRAYKAAASLPVMIQA
                     NAGRPELVDGAAVYRLAPEAFAEGMAPVVEHGAAILGGCCGTTPAHIEALHRAYGTRP
                     EKKLE"
BASE COUNT         2798 a         6577 c         6531 g         2737 t
ORIGIN      
        1 ccgcaacgca ggacgcgcga cagatcggtc ccggccgcct cgaggatcgc ctggatgttg
       61 cggaacacct gctcggtctg ggcggcaaca tcggggccga ccatccggcc agtggccgga
      121 tccaccgctc cctggcccga cacgaacacg aggcggtcga ggacgattgc cggcgagtac
      181 ggacccaccg gcgccggaag gttgggggcg gccacgggac gggcagtcgt cgaagtcacc
      241 tcgcccccct agaagccgaa cgcgaacgac acgccgaaca ggttcgcctt gaaggagtag
      301 acgccgttgt tggtcgtcgg gttggtgcgc cccgcccgct ccggctggct gaggtagagg
      361 tagaacgcat ccacgcgcag gttgtcggac agccgctggc cgatgccgac cgagaactcg
      421 cggcggaccc cctcgggcag gttcggcgtc acggtctcgt cgggcgccgc cgccgtatgg
      481 aagtccaccc ccgcgcgcag ggtggtcgcc tcgctcagcg cgtactcggc gccggcgcgc
      541 acaccgtgcg tgtcgttgta attctcggga accgacgact gcaggaagtc gccgttgacc
      601 ggcagttcgt cgaacgccga ccagttcacg aactggtagt cggccaggag tttcagggcc
      661 ggcgtcgcct ggtaggcaat cccggccacc agctgcgccg gcagcgggat ctcgctctgg
      721 gcgccctggg gccccagcaa ctggcccggc tggaaccggc tggccagcag gaggtccagc
      781 ttcgtgccgc gcggcgcgac gcccgggatg gcgaccggca gcacgaacgg cgtgggaatc
      841 tgacgcgtct cgatcgttcc ttcctcgatc tccacgctct gccccgtcat gaaccgcgct
      901 ccgagggaaa ccttctcgct cgccttgtac aacacgccga agtgggcgcc gacactccac
      961 gcgtccccct cgaggttgat gtcggcgaag tcggtcccgg cccgcacgcc gagcgtcccg
     1021 aaggtcgcgc cgggaggcag gccgggcacc gcgggcagcg cctgagccga gaggtcgagc
     1081 cgctggcgca gctgcacgtg gagcctgacg aggtcgatgc cggcgcccac cgacagccgg
     1141 tcgttcacct tgtaggacgc cgtcggctgg atgtaaaccc cctggacgac gctcttgtag
     1201 cccaggaagc ggccctgcga ttcctcgggc cagtccgtcg tcagcccgta gggcgcgaag
     1261 atcccgatgc cgacggcgaa ccggtcgccg atcggcgtcg acgcgtagac gttggggagc
     1321 ggataccact tcttgttcag cgagctgacc ttcccggtgg tgtcgtcggt gaagtcgccg
     1381 cgcgggccga tcaccgtccc ccccaggcca atctgggtcg cctcgaacga cagcccggcc
     1441 ggattgaagt acacgctcga cgcgtcggga cagggtgagg ccacgccggc gccgccgcgg
     1501 cccagctggc aggggctctg ctcgtatacc ccgaagccct gcgccgaggc ggcctgggcg
     1561 gcgcccagca cgcagacgac aaccgccaag gctcgcaaca gtaagaatcc acgcatcttt
     1621 cgcgtcctcc ggccttcaca gcttgttctg cgccggcccg caggctgcac ggcggggcgg
     1681 cttcttttga ttcggggctg cgcggcgaaa gggagaaggt agcacagatg ggctggagcc
     1741 cggacggatt tcctagcgcc aggagcggtc gaagcgttcc tcgaagacgc gggggtcttc
     1801 gccgaaaacg gcgcggaagt tcgcccggcg gtcgtcgagg gaggcgaacc tctcgaagta
     1861 gtcgacgagg gcaccgaagc catgccggtc gatcagctcg gccgccgcca cgagcgacac
     1921 atcgtacatc gggtcctcga ggtcctcgcc aaccagctgg acccacgagt cggcggacac
     1981 gaggtcggac aagcgcggca acgccgacct gcaggaggcc tgacgcaggt ggccgcgcac
     2041 ggcctccacc ctcgaggcga ccgtctcctc gccgaggacc tcgagcaccc gggcaccgac
     2101 ccattccgcc atgccctcgc gcaaccactg ctcggtcccc atccgccgcc cccctgccag
     2161 ctggtattgc gcgacgtgcg ccatctcgtg ggccagcgcg gcaattcgca ccggccagtc
     2221 gtgcgggtgg agccggcaga ggttcaagca gatgcggtcg gcggtcgaaa cgcccaccat
     2281 cgtcggcagg gaggatgcgg ggggcagcgg ggcgtggcgc ccgtcgccgg ccagctcgtc
     2341 ccgaaacgcc tcggcgctgg agaacagaac gatctcgccc cggagcggaa ggcgcaactc
     2401 gcgttcgacg accgagagcg cggcggcggc ggcccagcgg taggtgcgaa tcgtggggag
     2461 acggtgccgg gggactcgcc gaaggtcgtc gaggcgcatc accctggccg acgcgcccgc
     2521 tccctcatct gccggctttc gaggggcccc cttcgcctgg aggccggttg ccaggcacac
     2581 ggcggccaca acggccccga gccggccagg ccacctcttg agaggcgagc ggacgcgggg
     2641 gcatgaaggc ggcatgcgca tccctgctcg cagaaaccgg ttaacgcgtt gtcggtgaag
     2701 ggggcggaag aggacttctt tgtacgccgt gagagtcgcg aagtcaaccc tcgcccgcga
     2761 gagcgggcgg tgcccgacct cgtttgaccg ggcaacccgg agttgctagc atctcgaggt
     2821 caagccttca tcgacctcga caggagcctc aatgaccaga cgacaggttc tgcgcgctct
     2881 gttcgcatcg tcgatcgcgg cggcaggcgc ggcgccggcg ctggccggcc aggacaagaa
     2941 gccggctgag aaggtgaaag accccgtctg cgggatgatg atcgacgcgg cgtcggccaa
     3001 gggcaagtcg gagcacgagg gcaagacgta ctacttctgc tcggacagct gcaagaagaa
     3061 gttcgacgcc gatccgaaga agtacgtgca gccgcccccg ccgcccaagc gctgatcgga
     3121 tccccgcggc aacgggcggc tcgcgcgagc cgcccgccgc ctccttcccc ctacgcgctc
     3181 acgggccgcg cgatccccgg gcaggggaag ccggcacaga actcgcgcac ctcgccggcg
     3241 atgcgatcga gctcggcctc gtcggccacg tgctcggcca cgcggttgaa ccagccggca
     3301 atcctcggca tctcggcttc cttcataccg cgagaggtca ccgacggcgt cccgagccgc
     3361 aggccgctcg ggtcgaacgg ctttcgcggg tcgccgggca cggtgttgta gttgcactcg
     3421 aggccggccc ggtcgagcgc ccgcgcgtac gccttccccg tcatcccgcg cggcgtcacg
     3481 tccatcagga tcaggtggtt gtcggtgccg cccgagatca gcttgaagcc gtatcccgcc
     3541 agcgcctcgg caagcgcccg tgcgttcttc acgatctggt gggcgtaggc cgagaactcg
     3601 ggcgtcgccg cctccttcag cgcgacggcg atccccgcgg tcgtgtgatt gtggggcccg
     3661 ccctgcagcc ccgggaacac cgccttgtcg atggccttgg ccatctcctc cttgcacagc
     3721 agcatggcgc cgcgcgggcc ccgcagcgac ttgtgggtgg tcgtcgagac gacatgagcg
     3781 tgagggatcg ggctggggtg agcgccgccg gcgacaagcc cggcgatgtg cgccatgtcg
     3841 accagcaggt gcgcccccac ctcgtcggca atccggcgga acgcctcgaa gtcgatgatc
     3901 cgggggtagg ccgttgcgcc cgcgatgatc agcttcggcc gctccttcaa ggcgagctcg
     3961 cgcacctggc cgtggtcgat caggccggtc gcaatgtcga cgccgtactg gaccgagcgg
     4021 aagaacgccc cggtgatcga cacgctccac ccgtgggtca ggtggccgcc gtgcggcagg
     4081 gccatcccca tcaccgtgtc acccggcttc agcagcgcga agtacaccgc gaggttcgcc
     4141 ggcgatcccg agtacggctg cacgttcgcg tgatcggcgc cgaagaggct ccggacgcgc
     4201 tcgatcgcca ggcgctcgag cgggtccacc aactgctgcc cctcgtagta gcgcttgccc
     4261 gcatacccct cggagtactt gttggtgaac acgctgccgg tcgcctcgag cacggcgcgc
     4321 gacacgtagt tctcggaagg gatcaggcgg atcttgtcga actcccggcg ctcctctgcc
     4381 gcgaggaggg cggcaatctc ggggtcggcc tggagcaggt gcgggtcgag catggtgtcc
     4441 tctcttcggg gagcgtcaca ccccgccatc atcgttctcc gctcggagcg acgtcaaggc
     4501 aggacgcgga accctcttct cgaaccaggc ggcgaaccgg gcgctggtcg cgtcgaggcc
     4561 ggaggtgggc accggccggg acggggccga cgccgaggaa acggcgggga cgggcggcag
     4621 gggaaagatc ccggcgtccc acagcccctc gggcccttcc gcgtccaccg tcacctggcg
     4681 gtagataggc gcggcacgcc tgatctcctc gaagatctcg tccgggctgg cgtggtcgaa
     4741 tccccggccc cccagggcgc gtcccagggc gcacagcacc tgccaggtct ccaggcccga
     4801 gggcggcggg atcacgcggc gaaggcgctg gacgcggcgc tcggcgttgg tcatcgtacc
     4861 gctggtctcg acgctgctgc tcatgggcag caccacgtgc gccgcccggg tcgtcgcggt
     4921 gggaaagacg tcgccggcaa tcagcagatc ggcagcggcg aggccggctc gcaaggcgtc
     4981 aggaatgccg gacgcaccga gcgggtcgat gccgaacgcg agagcgacct tgatccgccg
     5041 atcggcgagc tgccacgcga ggtcgggtgc cggagaggac agcgcgacca gcgggacgcc
     5101 ccacaagcgc tcgagatcgc cggcggcgcc accgtgccgc ggacggtagc cggggagcca
     5161 ggcggggtgc gcacccacgt cgagcaaccc ctgcgcgttg gccccctcgc gggccggcag
     5221 cagcgcgcag ccgagcgccc gcgcagccgc cgcatacgcg cgcgcgtcgc cggtgcggcg
     5281 ctccccccgg taatcggcgt cgaacacgaa cacccggcgc gaggccgccc ttacccgctc
     5341 ggccgccctc tcgatggctt cggccttgac gccggtccac tcgagccact gcgaggggcc
     5401 gagcgaggcc accatcccca ggtcggggtg gcttaccggg tcctcgcccg cgcggcgcaa
     5461 agcggccgcc gcaatcccgg ccgccacgag cggctccgca cccggccggc actgcaggca
     5521 cacgtcggcg aggtcggccg tccggtcggg ctcggggcca atccagacga tggaggcgcc
     5581 gccgcgcgcg gcacgcgcgg cggcgaggtg cagcgtgaag cgctcctcgg ccagccgcgc
     5641 gccggccacg acgagggcat ccgcggcggc caggtcctgg taggtagcgg acgcgatcgc
     5701 ctcgggggcg aagtacgcct ggttcaccag cgctccaaac gaggcgacct gatgggtgcc
     5761 gaggatgccg cgcgccagct tctgcgcgag gtaggcctct tcgttggtca tccgcggcga
     5821 cacgaagacc gcgatctcgt cggggcggca ggtggcgagc gcccgcgtca ggcgatcggc
     5881 ggcagccgcg atcgcctcgt ccacgcctgc ctggaccgcc gcgccgttct cgcgcacctg
     5941 tcccgccgtg agccggccgg ggccgccgag gtggtggaac ccgaaacggc cctgccagca
     6001 gtgggtgccg aagacgacgt ggtcgtcctc gcggcgcgtc gcgctgacca gcgtgtcgcc
     6061 gaggctttcg aaggagaggc ggcagccgac gctgcagtag ctgcagacgc tcgcggtgcg
     6121 atcggtcttc caggggccgg gcttctcgag cgtcagccgc tccgcgatcg cgcccgtcgg
     6181 gcaggtgccg atgcagaggc cgcacgagat gcagcccgca tcgaggagcg atccgcccag
     6241 agccggcgcc acgatggtgc cgaacccgcg gttgacgaag ccgaagaccg agacgcccac
     6301 gacgtcgctg cacatccgga cgcagcggcc gcagaggatg cacttgttcc gatccagctc
     6361 gatcagcggg tgggacaggt cgcgctcgtg ctggcgcgcc tcgccgagga agcgcgacac
     6421 ctcgacgccg tactccgtgg cgtacttgcg gaggtcgcag tcgaacagcg ccacgcagcc
     6481 gcactcgagg cagcgcgccg cttcctcgtg cagatcgtca ccggtgtagc ccagctccac
     6541 ctcgacgaag cccttgaccc gctcctccac cgggacgagc ggcatcaccc gccgcggctt
     6601 cgactcgcgc gagcgaaggt cctcgatcgt gatcttcgcg aaaacatcct tgcggctcag
     6661 gaacggctcg ggctcgggac gcgcccttcc ctcccgcacg taggtgtcga tgccatacgc
     6721 cgccttgcgg ccggcggcaa tcgcctcgat cgcggtggcg gcgccggtca cgacgtcccc
     6781 gccagaaaac accccttcga ccgtcgtctc cagggtcttc ggatcgacct cgaccgtctg
     6841 ccaccgggtg agtccgagcg actcgccggc gggaagcagc ccggggacgt ggcccaggag
     6901 gtcggcaatg gtggcgctct gaccgatcgc agcaatcacc aggtcgcacg gcacctcgtg
     6961 ctcggagccg cgcaccggac gcgggctgcg ccgccccgag gcgtccgggg cgcccagctc
     7021 catgcgctgg cactccaggg cgcgcaggcg gaggtcggcg ccgggcacga cgcgggtcgg
     7081 agccacgagg tactcgatct gcaccccctc ctcgattgcg tcctggatct ccgagtcgtt
     7141 ggcgggcatc tcggcgcgcg tccgccggta gagcagcttg acggtggacg cccccagcct
     7201 gagggccgtg cgcgcgcagt cgatcgcggt gttgccgccg cccaccaccg ccaccgtgcc
     7261 ttgcagcgcc atcggcctgc cgaggcccac gtcgcggagg aagtcgatcc cggcgacgac
     7321 gccgggagac tcctcgttct cgacccgcat cagcgagctg cgccacgcgc cgatgccgag
     7381 gaagatcgca tcgaacccct cgtccttcag cgacgcgatg gtgaagtcgc ggccgagcgc
     7441 ctggttgggg tgcagcgtgg cgccaagcgc caggatctgc gcgatctcca ggtcgagcac
     7501 ctgtttcggc agccggtact ccgggatgcc gtaccgcagc atgccgcctg gctcgggctg
     7561 cgcctcgaag atcgcgaccg cgtgcccgcg cagggccagg tagtaagcgg ctgagaggcc
     7621 ggcgggaccc gcgcccacca ccgcgatccg cttgcccgtc accggcgccg gctcggcccg
     7681 ccaggggctg gcgccggcga gatcgaggtc ggcgatgtag cgcttgatgt agtcgatgcc
     7741 caccgcctcg tccagcagcg tccggcggca gcccttcacc tcgcagggcc gcgtgcagac
     7801 gcggccgcac accgacggca gcgggttgtg ctccttgacg agcgcgatcg cttcgcggta
     7861 cttgccgatg gcggccaggg cgatgtagcc ctggatgtcg atgccggccg ggcaggccag
     7921 ctggcaggga ccgacgcagt cggcgtagtg gtccgacagc agcagctcga gcgctgcctt
     7981 gcgcgactgg cgcacctcga ggccgttggt ctcgacgacc atcccgtgcg cgacccgggt
     8041 cgagcaggcg ggcagcaggt tgcgcgcccc cttcaccttc accacgcaga ggtagcagga
     8101 ggcgaacggc tcgagctggc ggtcgtggca gagggtgggg atcgagatcc cgacgcttcg
     8161 cgccgcgtcg aggatcgtct cgcccgcgtc cgcactcacc cgccgcccgt cgatctcgag
     8221 cttgactcgc gccataacac gtcccgaatc ctgaatcctg aatcccgaat cccgaatccc
     8281 gaaccccgat cactcccggt ggaccgcgcc gaggttgcag accttgtagc actcgccgca
     8341 gacggtgcag agggcctggt cgatgacgtg caccttcttc cgctctccgg aaatcgcgtg
     8401 cgccgggcag gccttgaggc acagcatgca gccgctgcaa tcgtcggtga tcgtgtaggt
     8461 gagcagcttc tggcagtggt gcgcggggca cttcctgttg acgatgtggg cctcgtactc
     8521 ggaccggaag tacttgagcg tggtgaggac ggggttgggc gccgtctggc ccagcccgca
     8581 cagcgacgac tggcgcacgc gctcggccag ctcctcgagc agcgggatgt cgctctcctt
     8641 tccgtcgccc tcggtgatcc gggtgaggat ctcgagcatc cgcttggtgc cgagccggca
     8701 gaaggtgcac ttgccgcacg attcgcgctg ggtgaactcc aggaagaagc gggcgacgtc
     8761 caccatgcac gtcgtgtcgt ccatgacgac caggccgccc gagcccatga tcgcgccggt
     8821 gcgggtgatc gactcgtagt cgatcagcgt tgcctcgagc tccgaggaga tgcagccccc
     8881 cgaaggtcca cccatctgga cggccttgaa cttgcggccg tcgcggatcc cgccgcagat
     8941 gtcgtggacg atctcgcgga tggtgatgcc catcggcacc tcgaccaggc cgccgtgccg
     9001 gatccggccg gccatcgcga agaccttggt ccccttgctc ttctcggtgc cgagcgcggc
     9061 gaacgcgcgg gcgccgttca gcacgatcca gggaatgttg gccagggtct cgacgttgtt
     9121 gatcgaggtc ggcgcccccc agagcccctt caccgccggg aacggcggcc gcacgcgcgg
     9181 catcccgcgc ctgccctcga tcgaggcaat gagcgccgtc tcctcgccgc agacgaaggc
     9241 gccggccccc tccttcagcg tgacgtggaa ggagtgccgg ctcccgagga tcgagccgcc
     9301 cagcaagccc cgctcttccg cctggcgaat ggcgatcgtg aggcgctcga tcgccttggg
     9361 gtattctgcc cggacgtaga tgtagccgtg gtcggcccca accgcgtacc cggcgaggat
     9421 catcccctcg agcaccgagt gcgggtcgct ctcgaggacc gagcgatcca tgaaggcgcc
     9481 tggatcaccc tcgtccgcat tgcacacgat gaacttccgc tcgccggggg cctgccgggc
     9541 gtaccgccac ttgacgccgg tcgagaaccc ggcgccgccg cggccgcgca gccccgactc
     9601 ggtgatcgtc gcgatgagcg cgtcgggctc gatgccggcg agcacccgct cgagcgcctc
     9661 gtatccgccc gccgccaggt actcgtcgat cgactcgggg tcgatggtgc cgcagttgcg
     9721 cagcacgatg cggtgctggc gggcgaggaa cgcctggtcc gagccgcccc cccggtcggt
     9781 ccacaccagc cactcggcca ccggacgccc ctgccccacg tgctcgtcga gcacgcgatc
     9841 aagcctgtcg ggggtgaccc ggccgtacag gtggcgggcg ccgtcgcggg tgcgcacctc
     9901 caccagcggc tcctggtagc acatgccgat gcagcccgtg cggacgacct ggaggccggg
     9961 gcggccagcc gccttcgcct cgagcgaccg gaacgtctcc tcggcgccgg ccgcgatccc
    10021 gcaggtcccg agaccgaccg tgaggcgggt cacggccgct cccccttctg ccgcttcttc
    10081 agcaccttga ccgcgttcgt cgggctcagg ttcccgtagg tggcctgatc gatcatcatc
    10141 acgggcgcca gcgagcagca gcccaggcac gacaccgtct cgatgctgta ctggccgtcg
    10201 gcggtggtct cgccggtcgg cgcaccgagg tgcttctcga tcgcctgcga cagctcgacg
    10261 gcgccgctga cgtggcaggc ggttccatgg cagacgcgca gcaggtgctt gcccaccggc
    10321 ttcagcctga actgcgcgta gaaggtggcg accccgtaga tgtgggcgag cgggatcccc
    10381 gtgcggcggt ggatctccac cagccgctcg cggcgcaggt acccgtcctc ctgctgcgcc
    10441 cgctgcagca gcgggatgag tgccccttcc tcgccctcgt agtggtggtt ccgcaggtcg
    10501 tagcgccggt gctgctgcat caggaccctt gttgtgtgag agagggaggc agtttacccg
    10561 gggtcggatc ccgagttcac cacatttctc acgccgcaac cccggcagta cctcgagttg
    10621 gtctgacgca aagaagcggg gcggcctggc gcctcagcgg ttgaccgggg cctgcggcgc
    10681 tgaacgcttc tgcagctgct ccaggaggcc cttgagcgcc tcgaggcgct gccccgcctc
    10741 tccgaggcgg ccctcggcgg tcagccgctg gtactcggcc agctcgcggg cggcccgatc
    10801 gatcaacgcc tgcaccgacg gtggagtggc cggaggtgcg gcatctccag gctctgccgt
    10861 cgcggcgggt tcggcgacgg gcggggcctt ggtacccgcg ccgaagaggc tggtcatcgc
    10921 cgcctcgagg gacgaaccgt agccgatccg atcctgcagc gccagcacga cgagccgcag
    10981 ctgcggcatc gggctccgct cggcctgcag gtagatcggc ttcgcgtaga gcagcgcacg
    11041 accgatggga atcaccagca ggctcccccg cctcacgttg gatccctggc ggctccacag
    11101 tgtcagctgc tcggacagct cgggactctg gtcgatccgg gcctccacct gcagcgggcc
    11161 gtcgacgagc cggctcttcg agaagttgta gacgcggacc cggccgtagt gctcgccatc
    11221 gcttcgcccg gccatccacg cgatcatgtt gttgcggttg gcgggggtga acgggaggat
    11281 ctcgacgaac tcgagggcgc tctcgcccgg caggcgcatc acgatgaagt tcggctcgac
    11341 ggggcgcgcc gcctgctgcc gaccctcgcc cgacaccacc tcgctcggca cgctccagag
    11401 gtcctcgcgg ttgtagaaaa cgtccgggtc ctccatgtgg tacaggctgt aggccgcggc
    11461 ctgcacctcc agcagcagct cggggtaacg cacgtgggcg cgcaggtgat cgggcatcgc
    11521 gctcgcgtcg gcgaacagcg tcgggaagag gccgcggtac gcggcgatga tcgggtcgtc
    11581 cggctcgaac acgtaatacg tcatcgtccc gtcgtacgcg tccacgacga ccttcacgct
    11641 gttccgcagg tagttcagcg gccgcccccc gaggcggtag cgccgggcgt acgggaattg
    11701 ggccgacgag gtgaagccgt ccatcatcca gtacagccgc ccgtcgtcgc cgacgacgat
    11761 gtacgggtcg gagtcgtaga tgagaaacgg cgccacggcc cgcgcccgct ccctgatgtt
    11821 ccgccgcatg agcaggcggc tctcgttggt gacgtcgtcg ctgaagggga gcttcgagag
    11881 gtcgccacgg ttgaacgcca gcagcagccg cctcgtcagc gagtggatcg ggatgccgcc
    11941 cttgccgtcg taggtggtgt acgcgttggt ctcgccctgc gggtagttga actcgcgctg
    12001 gcgcgtccgg acgtagacgt cggtgttggt gatctcaccg aagtagattt cgggacgggt
    12061 caccgtgatc tcgggcagcc ggctctgcac gggcatgtcc tggagcacca gttcgggcag
    12121 cccttccggg gtgaacccgt tcaccgtgtt catggtcacg ccgtagccgt gcgtgtaaat
    12181 cagcttctcg ttgatccagt tgcggctgct ctcgggcagc ttctccaggt tcatctcccg
    12241 cgccgtgacc atcatctggc gcacctggcc gccgacgcga taccggtcga ggtcgatgtc
    12301 ggggaagtcg taataggtcc ggatctcctg gatctgccgc agcgtgtcct gcagcggccg
    12361 ccagtcccac agccggatgt tgtcgagcgt ctcgcggttg ccggccaggt cgagggcgtc
    12421 cacgctggtc tcggccggga acgagcgctc ctccatctgg tcgagcgcga aggccatccg
    12481 cgtgaaacgg atgttgttgg agatgtacgg gcgctcccga accagctggt tcggcttcac
    12541 cacgaagccg gcgacgtacc acgacgtgat cgacagcacg acgtagacca gcaccgccgg
    12601 cgccagcgcg gccgccagtc ccgcggcgcg gcggaagcga agcccgttca cgatcgcgac
    12661 gaccccgccg agcaccagcg cggccgacac ccagagcatc cccgggatga ggatcgtcgc
    12721 ctcggtgtag cccacacctg agaacaccgt gtggtcgtcg aacaggcgct cgaagcgcga
    12781 aaggtagacg cgtcccgcca tcaccagcag cagcacgccg agcgtcagcg aaacgtcccg
    12841 gtagcccacc cggccgttcg gcagcccgag catcgtccgg ctggcctcgc ccgacacgac
    12901 gaaggcgccg agcgacgcga ggagcgccac gaccgcgacg gcggtcagcc acccgaacgc
    12961 gaggtgaaga accgggagcg tgaacaggta gaacgggatc ggccggccga gaatcggatc
    13021 gacggtcgag aacagcccgg aggcggcggg ggcgtgcagg tagagagcca cctcgtccca
    13081 gcgctgcacc acggtcgagg cggcgacgag cgcgaccagc gccgccagca cccacgagac
    13141 gatgttgacg accggctcga gcgagaaccg caccggctgg ttgttgacgt agaaagtgct
    13201 ttgggcaagg ttgcgcgggc ggaccaggcg aaagacaccg aacaacagaa ggaaggtggc
    13261 cagcgccacg ccccagaaga gcgcggtctt gtacgtgatg gtggtccaga agacgtcgcc
    13321 gtagcctagc gagtcgaacc acagctcttc tacgtaccag gaaatcaggg tccccgcgcc
    13381 gaggaacagc agcagggcga gcgcggcaat tgccacccac gcccgccggc cgcgccggct
    13441 ccccacgaag acgtcgtcac gataacggcc ggcgcgcggg aactccatgc gtaggtgtcc
    13501 tttcgccgtc aggcggcccg gcacgggccg gttgccggac accatgatac gacaggcgcg
    13561 gcgggcgccc cggagagggg ttggtctcac ttacaatgcc cgacgtggga ccggtgcgcc
    13621 gcatcctgca cgtcgacatg gacgcgttct acgcctcggt cgagcagcgc gatcggcccg
    13681 agctgcgggg ccggccggtc gtcgtgggcg gccggccgga cgagcggggc gtggtggcgg
    13741 cggccagcta cgaggcgagg aagttcggca tccactcggc gctgcccatg gcgagggccg
    13801 tgcgcctctg ccccggcgtc gtgatcgtcc accccgattt cgcgaagtac cgggccgtct
    13861 ccgggcaggt cttcgagatc ttccgttcgg tcaccccgct ggtcgaaccg ctgtcgctcg
    13921 acgaggctta cctggacgtg acatccaacg cctggggcga acccctcggc gtagacgtcg
    13981 cgcgccgaat caaggcgctc atcttcgagc gcacggggct gacggcctcg gccggggtgg
    14041 cgccgaacaa gttcctggcg aagatcgcct cggggtggaa gaagccggac ggcctcacgg
    14101 tgatcggccc ctcgcgggtc gagtcgttcc tgcaggggct gacggtggat gccctctggg
    14161 gtgtcgggcc cgtcaccgcc cgaagactgc acgacgccgg gatcaggacg gtcatggacg
    14221 tgcgggcggc gggcgagacg cgcctgcacg aggcggtcgg aagcctggcg ggatggctcg
    14281 tgcagctggc gcgcggcgag gacgaccgcg aagtcgagcc cgagcgtcct gccaagtcgt
    14341 cggggaccga gaacacgtac gcgcaggacc tccgcgacat cgatcgaatc cgcgccgaag
    14401 tgacgtcgat ggcgcgcgag ggggccgcct ggctgctccg gcacgagctg tacgcacgca
    14461 cggtcaccat caaggtccgc tacggcgact tccagacggt gacgcggagc cacagcgcac
    14521 gtccggccac ccgcgatgcc gatgcgatcg cggcccgtgc cgtggagctg ctcgcgagaa
    14581 cggaggccgg gaagcggccg gtgcggctgc tgggggtcag cgtccacaac ttcagcgaga
    14641 cgccgggcga agcaccgccg gcgcgccggc gcccgctgcc cttgttcgac cccttcgacg
    14701 gctaggattg gaggacacgg gatggaccag acgagggcgg ccgcgtaccg cgaggccgcc
    14761 gccgcgaatc ggcgcgagcg cgacgggtgg agccgtcggt cgcgccgcat cgccgtcgca
    14821 cgggtggcgg tgttcctctc ggcctcggcg ctcacgttgt gggcactcca gggccgcggc
    14881 gagtggtcgg cgccggccct cgccaccggt gcgttgggct tcctcggctt cttcgccctc
    14941 gtcgtctggc acagccgcgt ggagtccgcc gagcggcggg cctcggcgtt cgcgcaaacc
    15001 aacgaggagg ccgcgctccg catcgagcgg cagtggagcg cacttccccc gccccgcccg
    15061 tcgcccgccg atgcggcaca cccctacgcg gacgacctcg acctgttcgg ccgtgcctcg
    15121 ctgttcagct tgctcggatc ggccgggacc gaggccggca ggcaggcgct cgcccgctgg
    15181 ctcctccatc ctgcgcgacc cgaggagatc cgcgagcgcc agcaggcggt gcgtgaactg
    15241 gccggccgcg aagcctggcg tgaggcgctg gccgcgacgg cacggatggt ggaggccaat
    15301 ccccacgaac tggacgtgtt cctggagtgg gcaacctcct ccccgtggct cacgccgaag
    15361 cgttggctgc tctggaccgc gcgggccgcc accgccacca cgctgccgct ggcggctctt
    15421 cacgcgtccg gtgccatcgg ttccccgtac tggctggcgc cgttgctggt gtcgctcatc
    15481 ctgatggccg tgtacgagcg gcggatccac cagaccttct cgcaggcgtt ctcgcgtgag
    15541 cggctcttcc acgagcacgc cgcgctgttc gagcaccttg ccggggcgga gtacgccgcg
    15601 ccgctcctgc gcgggctcgc cgaccggctg caggcgtcgg ggctgaccgc gccgcgggag
    15661 atggcggcgc tcggacgcgt gatgcgtctc gccgacttgc gattccaggc gctcttccac
    15721 ttcccgatca acgccctgac gctctgggac gtgcacgtgc tggcggcgct cgagcgatgg
    15781 caggcccgcg ccggggtcca cctgccccgc tggttcgcgg cgctcggcga gttcgaggcc
    15841 ctttcggcgc tggcgtggct ggcgcacgac cacccggcct gggcgttccc cgaggtccac
    15901 gagagcgcga accggctcga aggcgtggac ctcggtcacc ccttgctgcc cgacgggcgg
    15961 cgcgtcggca acgacgtgac cgtcggccct ccaggacagt gcctgctcgt cacgggctca
    16021 aacatgtcgg gcaagagcac gctcctgcgc gcgatcggcg tcaacgtcgt cctcgcgcag
    16081 gccggcggcc cggtgtgcgc gcgccggctc gccatgccgc cgctctcgcc gttcaccagc
    16141 atgcgggtgc aggactccct cgaggagggg gtttcgtact tcatggccgc actgcggcgg
    16201 ttgaagcttc tcgtgtcgga agccgagcgg gtaccgggcg acgggccgat gctcctctac
    16261 ctgctcgacg agatcctgca gggcaccaac accgccgagc gccaggtggc ggtccggcgc
    16321 atcatggccc acctgctcga gctgccggcg atcggggcca tcaccacgca cgacctcgag
    16381 ctggccagcg cgcccgaact ggccggccga tgccgggcgg tgcatttcag cgagggggtg
    16441 tcggcggcag gggaaggcgt caggctgtcg ttcgactaca cgcttcggcc gggtgtcgcc
    16501 acctcacgaa acgccttgaa gctcctgcaa ctggtggggc tcggcgagcc cgggacgcgg
    16561 gagacgtaga acgcaggatg tagaacgcag gatcactcgc cattcttcgc tgccgcggcc
    16621 cggttcagct ccagtaccgc gagcacgccg ccgagctggc ccgccgaggc ctcgtagccg
    16681 agtacggcgg ccacgcgcgt gccatccagc aggcacgcgt tgtccgagac gaacacccgg
    16741 ggcgtgcgga aaacgcgcga gcggaaccag gcgtggtcgc ggtgcccgcg ttcgttgaag
    16801 aacgtcagcc gccgcggctc gctcccgtcg cggttcatga cccagagttc ggtgcggacg
    16861 tcgcgacgcc agttgagccc gtccaccgaa cggaacttca cgttgaggtc gcgggacgac
    16921 gaccagacga cctggcgccc atcggccgag tagtgggcgt ggtgatccca gtcggccgtc
    16981 tgcgtgagcg gccgcaggcg ctcggccgcc aggtcgtact cgtagatgtc gaggctctcg
    17041 agccccatgc ccccggcggg gcttgcggtg atcagcagcc gccgcccatc cttcgaccag
    17101 tcgtcaacct ggtagaagcc gtcgtgcggc gccggcgcca gcacgcgtat gttgcgcagg
    17161 gtgggtgtgg agtcggcgat ctcgaggtcg gccaggtaga ttgcccagcg gccccactcg
    17221 tagcccttcg cgttggcctc ctcactttcc tggtcgggct tcaccgtctt gtcggcgacg
    17281 ttctcgacgg gatcggcatc cacgggaccg gcccaggcaa tctgcttccc gtccggggcg
    17341 acgatcgggg ccagcacccc gcgcggatcc ttgtagtcgg tcgcaaaggt ggtcagcggg
    17401 aacgccctgg cgccgcccac cgccacggcc cacaaattgg tgttgagggc cgttgccggc
    17461 tccgccatcc ggtcggcctt gcgcgtgcgc acgtcgtcgt tctcggcgga gaagaacagc
    17521 cactcgccat cgggatgcca gattggggcg ccgcggtgct tggttggcgt gggcagctcg
    17581 caggtgatgc aggccttctc acgtccgctc ggccgcgtgg tccagacgtc gaggtaccag
    17641 tcgcgcggat cgcggcgcgc gtaggcgagc cgcgtgccgt cggcggagag gtcgacgtcg
    17701 gtcacccact cgtcgagcag cgcaatcccg agttcgcggt agatgcgagg aaaccggggc
    17761 tgtgaagcag gcgcggcgtc aagcgccgcg acgacgatgg tcagggcgag cgccgcgcca
    17821 atcgcggcga gggtcctcat ggcgcccata ccatgccaca aagagttggc ctgcgccacc
    17881 tgccgcgaac ccgctcgccg gaaacccgcc gagaatccgg cagcactggc ggcggttcgc
    17941 aagccgcgcc ccggcaccac cgcgcgtggt gggtccgaac acgcggaaga caacgtgttt
    18001 ctcgaggcgc tcccctggca ccggccttgc gtcttagaat ggcgttgcct ggttcgccgc
    18061 ccgcaatcag gcgcctgcgg atgggggccc gcaggtgcgg ggttcgggcg gcgggcccgg
    18121 ttcctttcct tccctctgcg tcccttactc cagcttcttc tccggtctcg tcccatacgc
    18181 gcggtgcagc gcctcgatgt gcgcgggcgt cgtcccgcag cagcctccaa ggatcgccgc
    18241 tccgtgctcg accacgggcg ccatgccctc cgcgaaggcc tcgggtgcca ggcggtacac
    18301 cgccgctccg tcgaccagct cgggccggcc cgcgttggcc tggatcatca cgggaaggga
    18361 cgccgccgcc ttgtaggcgc gcacgaccgt cacgaagctg gccggctgca tgtgcgtccc
    18421 gcaattggcg cccaggatgt cggcgcccgc ggccgcaagc tcgcgggcgg cctgttcggg
    18481 cgacaccccc atcatcgtcc gcacgctgcc gttgggcagc tcgtcgaagg ccatcgacgc
    18541 agcgacgagc ggagcgccgg ccgcgcgggc tgccttcacg gcggcgacag cttcctcgat
    18601 ggcgctcatc gtctcgacga tgacgccctc tgcgccgccc tcg
//
LOCUS       DAOPQX010000028        18507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3630_length_18507_cov_4.886245, whole genome shotgun sequence.
ACCESSION   DAOPQX010000028 DAOPQX010000000
VERSION     DAOPQX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 18507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18507
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3630_length_18507_cov_4.886245"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PKK95_02810"
     CDS             complement(<1..147)
                     /locus_tag="PKK95_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOC17167.1"
                     /translation="MLPELSRDELHRYSRHLLLPEVGLEGQRKLKAARVLCVGAGGLG
                     SPASM"
     gene            complement(158..907)
                     /locus_tag="PKK95_02815"
     CDS             complement(158..907)
                     /locus_tag="PKK95_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOC17168.1"
                     /translation="MEPARSALAAGKPFPGVTAVFFDLDGTLVNTIPLIFESYRHTLG
                     VHLPGFDPPRDVIIGNLGRSLHAILRDYAVAAGATDPYAMTEAMVATYREFQREKIDV
                     LIQPYPGVRDMLTGLRGRGYRLGLVTSKVEWAARLTFERYGLGELLETFVFHDDTAMH
                     KPHPEPLLAAASKAGVSPGQAVYVGDSVHDMTAALTAGMRAIGALWGPFEPSALRRAG
                     ADALADQPMRLLDLLPGVAPAGASKGAAGEG"
     gene            942..2051
                     /locus_tag="PKK95_02820"
     CDS             942..2051
                     /locus_tag="PKK95_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC17169.1"
                     /translation="MRIEPFEMERMQSTWENQVEINLSESGVHPLTIDELVGEGGERD
                     DLLRLSLGYPQTNGTPALREAIAALYPGATAAHVEVTNGGAEANFISLLTLLDEGDEM
                     VMLSPNYMQVWGLARTLGRPVHRWWLREDGRRWRPDLDELGCLVNERTRLIAICNPDN
                     PTGARLQGAELDAICEIAARRGTWVLSDEIYRGAELDGQETPSIWGRYERAVVTSGLS
                     KACSLPGLRIGWIVGPETFVASTWATHDYTTIAPGAVNDFLATIALRPDRRAAILART
                     RRILRANWATVESWFSAHQKLFSFAPPEAGAIVMARYHRPVNSTELTTRLREEKGVLI
                     VPGDQFGMDGYLRIGFGGDPAQLAEGLARLRELLA"
     gene            2048..3121
                     /locus_tag="PKK95_02825"
     CDS             2048..3121
                     /locus_tag="PKK95_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17170.1"
                     /translation="MTADLVLVGFGNVGRRFVRLLDERRAELRDRHSLEWRVVGICTR
                     RHGRARNPAGLDALAAAAAVERGESLGEGPGGDANGDGAAFIRSAAGDWRRSGSANPL
                     VVVETTVLDVRSGRPAIDHVRAALEAGAHVVTANKGPVAFAYDELKELAASAGRLFLF
                     EGAVMDGIPVFNLAARTLPAVRVSGFRGVVNSTTNYIISAMEDGREFGDALAEMQAAG
                     IAEADASLDVDGWDAAAKAAALANVLLGARTSPLEVERTGIAALEGPAVRDVVARGMR
                     LKLVARGRRHATGGLRVSVAPEELPAGDLLAGLKGQQNAIVLETDLLEDVAIVQLGGG
                     LTQTAYALVSDLVALRNALPALP"
     gene            3366..3794
                     /locus_tag="PKK95_02830"
     CDS             3366..3794
                     /locus_tag="PKK95_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17171.1"
                     /translation="METSDIRRRLRTAFEQHRKEAAERRARADEAAIAYQGFLRDIAV
                     PVFRMVANVSKAEGHPFTVYSPADGVRLVSERNERDFAELWLDASLDPPRVATRVNKQ
                     VGRDLVSREGLLRGEAPIPGLTDEDVLAFLLREVVELSER"
     gene            complement(3846..5867)
                     /locus_tag="PKK95_02835"
     CDS             complement(3846..5867)
                     /locus_tag="PKK95_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOC17172.1"
                     /translation="MVGSLLTAIVRADGDALVMHVGERPYVVAPAGPVELSSRPLTLD
                     AVAGMLGQLLPAEGRAALDELGAIEHELPRGVSGTRDRFTVVAARGGDDIWIEVRRHR
                     KPAAPAVVVPTAVAVPPVPQPEARASEEPAPTAAPAVAASNEASAQPPPEAAAPSEAA
                     APGEEAPGAGEEAAEVEPEPAAVETVPEEVIQPAPAAVSPAVGALASRGAAVAAEATE
                     AVPEPVAASPEVPAPEPAPAPEPLFDAFEKPAEPASAPEPIEESAAARPEGLEEKVER
                     QPAPVVESIPAVVLPLARSPLRPEAPRVSASPRLAGIDRLLRLAAARGANTLYLMSQS
                     RPAVRVDGEIATLEGERALTAVEVESLLLDLAPERSREALENGEGTEWMSEVAEVGRI
                     RCQSFRDHRGPGGIFRIISARPASADQLGLSREIQALAAEPEGLILVAGPRASGKSTL
                     VSAFVDLVNEARSDYVITIESQIQFAHEGRGCLVSQREVRGDAGKVAVMTRSALRENP
                     DVLVIDDLRSPEVMGIAVDAVAAGHLVIAAMPAHTATAALVRLIDQAAPDRREQVRLT
                     LAEGLRCVVAQVLLHKSSGGRVAAREVLLNTPAIANLIAEGRVDQLPLALEGGRKHGM
                     APLNDALAAFVQSGVVDAREAYRKAYERQAFVAVLRREGLDTSFLERLA"
     gene            complement(5897..6352)
                     /locus_tag="PKK95_02840"
     CDS             complement(5897..6352)
                     /locus_tag="PKK95_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17173.1"
                     /translation="MTLDTSGWSGDGDFTTRLLAVLETLEEVVAVKVEDAPASSSGAG
                     YQFLANEVFVTFATTPRRVRRWPGLPLTRLVHEPRLTLAGLEARLAAAEGVGAPDYSD
                     EGMLQYLRTERIVQPYQTRGYKLVELVRVYQVQPGPARSIEVTPFGRHD"
     gene            complement(6421..8595)
                     /locus_tag="PKK95_02845"
     CDS             complement(6421..8595)
                     /locus_tag="PKK95_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC17174.1"
                     /translation="MRPRFAFVSLFVFTLAGLALLAQAPPDRCLVPPAIRDPILGEVS
                     GERAHLHVQMLAANRDRQASEYTDLFFETTYIRDRAAEAGLSDVQVDLFPGRDTWDAE
                     EGDLWMVQPERKKIASITTVPASLAIGSRTSDVEAELVYVGAGRQADYEGKDVAGKIV
                     LGAGPVGGVFGAAVNQRGAAGALGTATTALEGEPASYSLDQVGWSSVYAATDRAGFGF
                     QLSLRQFLELRNLVERGQKVVLRAHVRSRTYPGRQNVVSAAIPGSDPAAGELILVAHA
                     FETIATPGANDNCTGVGTTLEVGRTLARLIRDGVLPRPRRTIRFLWAPEISGTTQYMF
                     KHPELQERLHAALNFDMTGANPKTTDAYLRVKLTPDSRPSYLNDLIASLLRYVDQTNI
                     RSPQGANATFNYRLTPVAAITSGSDHSVFNNGGIPAMQFNYWNDSFYHSSGDRAEHAD
                     PTEMKRVAFMAAAAMYYLSTAGPAEARDLAWEAAANGQAWIAEVARQAAGLMAGDGEK
                     LARQHEAAQLKVAGAFQRAQGGVESVLALARDAEVGGAVRRLVGSLQAARDLNARLLE
                     GKYRERAAARGIAAVPQAAPGARERECDLLVPRRLFPVYSSEAQRRAPSPGTGQRGAG
                     RPAAPMGRMPGIARSEVANFIDGKRSILEIYRAVRAECGNLVVGSDDQKFAYVLSPDA
                     PDVDLEQVVAAIEALENNGTVEILKREQPRGKRRPGSSAPSP"
     gene            complement(8645..9853)
                     /locus_tag="PKK95_02850"
     CDS             complement(8645..9853)
                     /locus_tag="PKK95_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17175.1"
                     /translation="MKMSRCLAIILGLLLLAVAVPLADRGVQECTTAIFSASSTPDGR
                     PILWKNRDTDTLSNKVVFVNDKPYSYLGVVNADDPDGRMVWGGLNSAGLAIANSVAYN
                     LPQRGGEQADLEGIVMADVLRTCARVDDAERYFAANLGPDLGAQANFLVIDAKGGASI
                     FEVHNHGFKRLDATSSPQGYMANTNFSRTGAQDQGAGYLRFDRETALLEAAPPRSFTP
                     AFVLQTLARDLGHPLLANLAREEWKQFPADRPYWVHTNHTINRNSTASVLVVHGVKPG
                     QDPRLATMWVILGEPVTGVAVPLWVAAGAPPAPLWEGKEAALTTEAFRLKDVLRPLKT
                     RERREYADLARLDNASGTGWLPAVLEAERETLDQVSRLIAKNPSAAELAAFEKSAAEK
                     ALALLKGLGR"
     gene            complement(9939..10592)
                     /locus_tag="PKK95_02855"
     CDS             complement(9939..10592)
                     /locus_tag="PKK95_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOC17176.1"
                     /translation="MPRFGLVVFDLDGTLIDSIGDLAAAVNAMLAERGAGPLSRRAVA
                     GMVGEGARLLVERAMAAANLEGDGADALSRFLEIYDGLLPGETRPYEGIPEALGVLGR
                     RARLAVLTNKPTEASLKLLDALGLRPFFAEVVGGDGPFPRKPAPAGLLHLSAAAGVPP
                     GETVLVGDSTIDLLTAHAAGTAACIARYGFGQVTFDETRLRGGELMAVRPHDLCTLL"
     gene            complement(10597..11001)
                     /locus_tag="PKK95_02860"
     CDS             complement(10597..11001)
                     /locus_tag="PKK95_02860"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOC17177.1"
                     /translation="MYSVTKRIEFCYGHRLLDYDGVCKHPHGHNAVAEIEVRTASLDP
                     RNMVADFGDIKRTVKGWIDRELDHKMILRRDDPLVEPLRQLGEPMFLVDSNPTVELIA
                     QLIFNYTREQGFDVVGVRVWETPNAYATYQAG"
     gene            11070..11747
                     /locus_tag="PKK95_02865"
     CDS             11070..11747
                     /locus_tag="PKK95_02865"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOC17178.1"
                     /translation="MNAIAVLCSGGLDSAVLLAREARERPVLPLYVRSGLAWEAEERR
                     ALEHFLASLASSAAVLPLATVQVDVQDLYPGSHWALRGTPPAYDTPDEDVYLPGRNLL
                     LLSKAAVLCALRGINRIAVGPLAGNPFPDATPAFFESIGRAASLGLDHEIEVVAPFSR
                     MRKEDVIRLGVELGVAFELTLSCMNPSGGMHCGLCSKCRERRDAFAAAGVEDPTKYRA
                     RSPREIG"
     gene            complement(11764..12693)
                     /locus_tag="PKK95_02870"
     CDS             complement(11764..12693)
                     /locus_tag="PKK95_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOC17179.1"
                     /translation="MNRHALRPLAASFALCSSITLAGCMVSVDSSEYTAREEKRFEVP
                     QGVPDVTLATFDGSVEIRSWDQAAVQVEVEKRGPNQQMAEAIEVRAEQAGHLITLTVK
                     KPAGETGGFLRQSPTARLIALVPRRAKIAVRSGDGSITVERVEGDLDLDTADGSVRGY
                     DLAGRLRIHTGDGSVRLEQVSGWVDVDTGDGTVSLAGKLQSVKVVTGDGGVVVRAEEG
                     SEMADPWEVRTGDGGVRLEIPPGFAANLDASTADGRVRLEGIGEEADREPEPAGEEVR
                     DERSRRELKRQLNGGGKLLRLRSGSGTITVAGL"
     gene            complement(12746..13201)
                     /locus_tag="PKK95_02875"
     CDS             complement(12746..13201)
                     /locus_tag="PKK95_02875"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOC17180.1"
                     /translation="MKSLASELSAVAERLAGQPRMYADANVPAGLVTFMRVRLRWDVL
                     FVTEHDDLRRQPDTEHFRLAAKLRRTLLTLDRDYLDDRRFPPSATAGVLVIAAPDENG
                     LSRLLSRLDRAIFRPRPRRGKARVRPAAAPLPLEGRKLHAHPDWRPAPR"
     gene            13345..13824
                     /locus_tag="PKK95_02880"
     CDS             13345..13824
                     /locus_tag="PKK95_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17181.1"
                     /translation="MTIEQVLIAAAVIVLAGLALIGLWIYWKGRPFAQGDVFRASRLS
                     RGNRLFPTQVLITPHSVVHHTPQWVGKLEETIHLAHVASVRIDTNLMFSDVAIETSGG
                     TNPVVCHGHSKKDAVRMKALIEEYQSAYYRAPSQAPPAAPPPAATPPGTVASPPSSR"
     gene            13856..15226
                     /locus_tag="PKK95_02885"
     CDS             13856..15226
                     /locus_tag="PKK95_02885"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC17182.1"
                     /translation="MASDFGSFPARRDTFPRARPTPTLGDLPTRFRTLVQSPLRAGIL
                     RFMHSRPNDVFDVESLMQVFGRMRLDVDNCVGELANFGVVRRLPGQPPRFIAGRPEQE
                     AVANLLDDFLERRATVSTEDQSPSVQRFREMIGRDEKMLIVFEWIRTAAKSDIAVLIL
                     GPTGSGKEVVARMIHELSRRGAGRFQAVNCAALPDTLFESEIFGHERGAYTGAHDRKP
                     GRLELANGGTLFLDEIGDLSIVSQAKLLRALEDRGFERLGGNRTIEVDFRLISATNRP
                     LEQFVREGRFREDLYYRVNAFAIRLPSLRERQVDIPVLAQRFLARYCAGNGLPVDAKT
                     FSREALDALGAYPWPGNIRELESTVSRAALSAPGRVIRGSDVDFLHAGTEAIDPATVR
                     LASLREIERAHIVKVLEAVGWNKKEAATVLDISRGTLYRKITEYGLERQARALLSKNR
                     ANLRTA"
     gene            15503..17494
                     /locus_tag="PKK95_02890"
     CDS             15503..17494
                     /locus_tag="PKK95_02890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOC17183.1"
                     /translation="MSKNLGGGIRDAVAGASKWWRNLLAPAALYRAGGAAAAAAAWAF
                     AEGRPLYGLCALGLALAASRRCRVQSRQLAELARRERLRSDVNLATIEALALAIDAKD
                     KTTSSHIRRVQRFSAALAERMEMDPDDVLGVTTAALLHDIGKLAVPEHILSKPGPLTD
                     EEFQKIHIHPQVGFEIVEHVPFPYPVAQLVLCHHERWDGKGYPLGLRGDDIPLGARIL
                     AVADYFDSMMRDRPYHKGVSRELAVEMLRQEAGRALDPRLVQVFLDLLPGLPDVQEPR
                     SVPLPVVPRTGGRHLFESGEQSAFANIARAHQEIYALYDMAQALGSSLGVADTMSLLA
                     AKLSALVPFSACALFVREDDETLRCRFASGVDADHLRRVSVRVGSGLAGWVTRNRRSL
                     VNARPAADFETADLAPAPPSLRAALVCPLICAGEVIGTLSVYHTDPDFYRDEHRRLLE
                     RISEQAAAAISNSIVFEKTREASLSDPLTGLPNTRFMFNYLGRELSRAQRLRTQVSIL
                     VLDLDRFKNINDTYGHHVGDRALREVARVLREGIRPYDICVRYAGDEFIVVLADCGFD
                     EAEAKRAELQQAVDELVLQLDGGETARLSVSIGAAVFPQDGETYEALLATADGRMYRD
                     KRGRKRAVSRCAVREAASIDSPEASPAAVEKVRPFAKVG"
     gene            17531..18235
                     /locus_tag="PKK95_02895"
     CDS             17531..18235
                     /locus_tag="PKK95_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOC17184.1"
                     /translation="MPLLTLPISSSRRATPRNLAVRLDLGGRPFDFSAGQYVLLGQAG
                     GADRCPYSIASAPSEVAASGALEFLIQVDEHGSPGPHLPSLAPGRCLAVEGPAGSFTL
                     PAGLPDADFLFVAGGTGIAPIRSMLVEVLRTLPSARVTLVQAARSPEELSYGDEFRSL
                     AREGRIGLVESVTREAPPGWQGVRGRIAAAHLAPLVGGARTVAFVCGPDSLVEEVPRV
                     LGALGVSNTKTERWAG"
     gene            complement(18311..>18507)
                     /locus_tag="PKK95_02900"
     CDS             complement(18311..>18507)
                     /locus_tag="PKK95_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOC17185.1"
                     /translation="TRVKTALLNDPNVGGLRIDVDTTMGVVTLSGVARTPEEEQQAVA
                     IARRIPGVKDVKSTLVVNPK"
BASE COUNT         2672 a         6597 c         6524 g         2714 t
ORIGIN      
        1 catcgaggct ggagagccca gccccccggc gccgacgcag agcacgcgcg ccgccttcaa
       61 cttccgctgc ccctccagtc ccacctcggg cagcagcagg tgacggctgt agcggtgaag
      121 ctcgtcgcgc gacagctcgg gaagcatatc gccgattcta cccctcgccc gccgcccctt
      181 tcgaggcgcc cgccggcgcc acccccggca gcaggtcgag cagccgcatg ggttgatcgg
      241 cgagcgcgtc ggcgccggcc cgccggagcg ccgagggctc gaagggcccc cacagtgcgc
      301 cgatcgcgcg catgccggcc gtgagcgccg cggtcatgtc gtgcacgctg tcgccgacgt
      361 acacggcctg gcccggcgac acccccgcct tcgacgccgc cgcgagcagc ggttcggggt
      421 ggggcttgtg catcgccgtg tcgtcgtgga agacgaaggt ctcgagcagc tcgccgaggc
      481 cgtaacgctc gaaggtgagg cgggcggccc actcgacctt gctcgtcacg agcccgaggc
      541 ggtagccgcg gccgcgcagg cctgtcagca tgtcgcgcac gccgggatac ggctggatga
      601 ggacgtctat cttctcgcgc tggaactcgc ggtaggtggc gaccatcgcc tcggtcatcg
      661 catacggatc cgtcgcgccg gcggccaccg catagtcgcg caggatcgcg tgcagagacc
      721 ggcccaggtt cccgatgatg acgtcgcgcg gagggtcgaa gccgggcagg tgcacgccga
      781 gcgtgtggcg gtacgactcg aagatcagcg ggatggtgtt gaccagcgtc ccgtcgaggt
      841 cgaagaaaac ggcggtcacg ccgggaaacg gtttccctgc cgccaaagct gaacgggcgg
      901 gctccatccg ggtagcatat acggaaccgc gaggaggggg catgcggatc gagccgttcg
      961 agatggagcg gatgcaatcg acctgggaga accaggtcga gatcaacctg tcggagagcg
     1021 gcgttcaccc gctgacgatc gacgagctcg tgggcgaggg cggcgagcgc gacgacctgc
     1081 ttcgcctgtc gctcggctac ccgcagacca acgggacgcc ggccctgcgc gaggcgatcg
     1141 ccgcgctcta tcccggcgcg accgcggcgc acgtcgaggt gaccaacggg ggcgccgagg
     1201 cgaacttcat ctccctgctg acgctgctcg acgaaggcga cgagatggtg atgctctcgc
     1261 cgaactacat gcaggtctgg gggctggctc gcacgctggg acggccggtg caccgctggt
     1321 ggctgcgcga ggacggccgg cgctggcggc ccgatctcga cgaactgggc tgcctggtga
     1381 acgagcgcac caggctgatc gcgatctgca accccgacaa cccgaccggc gcccgcctcc
     1441 agggtgccga gttggacgcg atctgcgaga tcgccgcgcg gcgggggacg tgggtgttgt
     1501 cggacgaaat ctaccggggc gccgagctgg acgggcagga gacgccttcg atctggggcc
     1561 gctacgagcg cgccgtggtg accagcggcc tctcgaaggc ctgcagcctc ccgggcctgc
     1621 gaatcggctg gatcgtgggg cccgaaacct tcgtcgcctc gacgtgggcc acccacgact
     1681 acacgacgat cgccccgggg gcggtcaacg acttcctggc caccatcgcg ctgcggcccg
     1741 accggcgcgc ggccatcctg gcgcgaaccc gccggatcct gcgcgcgaac tgggcgaccg
     1801 tcgaatcgtg gttctcggcg caccagaaac tgttctcgtt cgctccgccc gaggcgggtg
     1861 ccatcgtgat ggcccgctac caccgcccgg tcaactcgac cgaactcacc acgaggctgc
     1921 gcgaggagaa gggagtgctc atcgtcccgg gcgaccagtt cggcatggac ggctacctgc
     1981 gcatcggctt cggcggcgat ccggcgcagc tcgcggaagg gctcgcccgc ttgagagagc
     2041 tgctggcgtg accgccgacc tcgtcctcgt cggattcggc aacgtcggcc ggcggttcgt
     2101 ccggctgctc gacgagcgcc gcgccgagct tcgcgaccgc cactcgctcg agtggcgtgt
     2161 cgtcggcatc tgcaccaggc gccacggccg cgcacgcaat cccgcgggcc tcgacgccct
     2221 cgcagcggcc gcggcggtcg aacgcggtga aagcctgggc gagggcccgg gcggagacgc
     2281 gaacggcgac ggggccgcgt tcatccgaag cgccgccggc gactggcggc gctcgggctc
     2341 cgccaacccg ctcgtcgtgg tggagacgac cgtgctcgac gtgcgatcgg gccggcccgc
     2401 catcgatcac gttcgcgccg ccctcgaggc cggcgcccac gtcgtcacgg ccaacaaggg
     2461 gccggtggcc ttcgcctacg acgagctgaa ggagctggcg gcgtcagccg gccggctgtt
     2521 cctcttcgag ggggccgtca tggacggcat ccccgtcttc aacctcgccg ctcgcacgct
     2581 gccggccgtg cgcgtctcgg gcttcagggg cgtggtgaac agcacgacga actacatcat
     2641 ctcggcaatg gaggacggcc gggaattcgg cgacgcgctg gccgagatgc aggccgcggg
     2701 gatcgcagag gccgacgcct cgctcgacgt ggacgggtgg gatgccgcgg ccaaagcggc
     2761 cgccctggcc aacgtgctgc tcggcgcgcg gacgtcaccc ctcgaggtgg agcggacggg
     2821 gattgccgcc ctcgaggggc cggcggtccg cgacgtggtc gcgcgcggga tgcgcctgaa
     2881 gctggtcgcg cgcgggcgcc gccacgccac cggaggcctg cgcgtctcgg tggcacccga
     2941 ggaactgccc gccggcgacc tgctcgcggg cctcaagggg caacagaacg ccatcgtcct
     3001 cgagaccgac cttctcgagg acgtcgcgat cgtccagctg ggcgggggac tgacgcagac
     3061 cgcctacgcc ctggtcagcg acctggtcgc tctgcgcaat gcgctgccag cgcttccttg
     3121 agcttcttct cgtcgaaccc gcggatcgcg tagtcgccga tgagcgtgaa cgggatgctc
     3181 atcacgccga gccgagcgag gtcgtcgaac gcgcccgggt cctcgtcgat gttccggaca
     3241 atgaaggtcg caccggctcg tgaaagaaac tctttcaccc gctcgcacga ccgtcaatgc
     3301 ccgccggaat agacgatgat gtcggtctca gccatccttg cccctgtgta gactattgta
     3361 ccgccatgga gacatccgac atccgccggc gcctgcgcac cgccttcgaa cagcaccgca
     3421 aggaagcggc cgagcgccgc gcccgggccg acgaggcggc gatcgcttac cagggcttcc
     3481 tgcgcgacat cgccgtgccg gtcttccgca tggtggccaa cgtgtcgaag gccgagggac
     3541 accccttcac cgtctactcg ccggccgacg gcgtgcgcct ggtctccgag cgtaacgagc
     3601 gcgacttcgc cgagctgtgg ctggacgcct cgctcgatcc gccgcgcgtg gcgacccggg
     3661 tgaacaagca ggtgggacgc gacctggtgt cgagggaggg gctgctgagg ggcgaggcgc
     3721 cgatcccggg tctcaccgac gaggacgtcc tcgcgttcct gcttcgcgag gtcgtcgagc
     3781 tgtcggagcg gtaggtcttc cgcgcgggcg gggctgaagc cccgctccca cggcacatcc
     3841 gcggatcagg ccaggcgctc gagaaacgag gtgtccaggc cctcgcgcct cagcaccgcc
     3901 acgaacgcct ggcgctcgta ggccttgcgg taagcctccc tcgcgtccac caccccgctc
     3961 tgcacgaagg cggcaagcgc gtcgttgagc ggcgccatcc cgtgcttgcg cccgccttcg
     4021 agggccagcg gcagctggtc cacccgcccc tcggcgatca ggttggcgat ggcgggcgtg
     4081 ttgagcagca cctcgcgcgc ggccacgcgg ccgccgctcg acttgtgcag cagcacctgc
     4141 gccaccacgc accgcagccc ttcggccagc gtgaggcgca cctgctcgcg gcggtcgggc
     4201 gccgcctggt cgatcagccg caccagcgcc gccgtggccg tgtgcgccgg catcgcggcg
     4261 atgaccaggt gcccggcggc caccgcgtca accgcgatgc ccatcacctc gggcgagcgc
     4321 aggtcgtcga tcaccaggac gtcggggttc tcgcgcagcg cgctccgcgt catcaccgcc
     4381 accttgccgg cgtctccccg cacctcgcgc tggctgacga ggcagccgcg cccttcgtgt
     4441 gcgaactgga tctggctctc gatggtgatg acgtagtcgc tgcgcgcctc gttgaccagg
     4501 tcgacgaagg ccgagacgag cgtcgacttc ccgctcgccc gcgggccggc caccaggatc
     4561 agcccctcgg gctcggccgc cagcgcctgg atctcgcgcg agagcccaag ctggtcggcc
     4621 gacgcgggcc tggcggagat gatccggaag atgccgcccg ggccgcgatg gtcgcggaag
     4681 ctctggcacc ggatgcggcc cacctcggcc acctccgaca tccactcggt gccctcgccg
     4741 ttctcgagcg cctcgcggct tcgttcgggc gccaggtcga gcagcagcga ctcgacctcg
     4801 acggctgtca gcgcccgctc gccttccagc gtcgcaatct cgccgtccac gcgcacggcg
     4861 ggccgggact gcgacatcag gtacagggta ttggccccgc gggccgccgc cagccgcagc
     4921 agccgatcga tccccgcgag ccgcggcgaa gccgagacgc gcggcgcctc gggtcgcagc
     4981 gggctgcgcg ctaggggcag aacgaccgcg ggaatggact cgaccactgg cgccggctga
     5041 cgctccacct tctcctccaa cccctcgggg cgcgctgcag cagattcctc gatcggttcg
     5101 ggcgcggatg caggctcggc cggcttctcg aaggcgtcga acagcggttc cggcgcggga
     5161 gcaggctcgg gcgcgggcac ttcgggcgag gcggcaaccg gctcgggcac ggcctctgtg
     5221 gcctcggctg cgacggcagc ccctctggag gcgagggcac cgaccgctgg cgatacggcg
     5281 gccggcgccg gttggatgac ttcctccggc acggtctcga cagcggcggg ctcgggctcg
     5341 acctccgcag cctcctcgcc tgcccccgga gcttcctctc ctggcgccgc tgcctcgctc
     5401 ggtgccgcag cctcgggcgg gggttgggca gaggcctcgt tcgaggccgc gaccgccggg
     5461 gccgctgtcg gggctggctc ctccgaagcc cgagcctcgg gctgcggaac gggcggcacg
     5521 gccacggcag tcggcaccac aacggcgggc gcagccggct tccggtgacg gcgcacctcg
     5581 atccagatgt cgtcgccccc ccgggcggca accaccgtaa aacggtccct cgtgccgctc
     5641 acgccgcggg gcagctcgtg ctcgatggcc cccagctcgt cgagcgccgc gcgcccctct
     5701 gccggcagca gctggccgag catgccggca acggcgtcga gcgtcagcgg gcgcgacgac
     5761 agctcgacgg gacccgccgg cgcgaccacg tacggcctct cgccgacgtg catcaccagc
     5821 gcgtcaccat cagcccggac gatggcggtc aagagcgaac cgaccacgtc tgcctctgcc
     5881 tcctcctcaa tgctcttcaa tcgtgtcggc cgaaaggagt tacctcaata gacctggcgg
     5941 gtccaggctg gacctggtac accctgacca gctccaccag cttgtagccg cgggtctggt
     6001 agggctgcac gatccgctcg gtgcggaggt actggagcat cccctcgtcg gagtagtcgg
     6061 gggcgccgac gccctcggcc gccgcgaggc gggcttcgag gccggccagc gtgaggcggg
     6121 gttcgtggac gagccgggtc aagggaaggc cgggccagcg ccgcacccgg cggggcgtgg
     6181 ttgcgaaggt cacgaagacc tcgttggcga ggaactggta gccggcgccg ctgctgctcg
     6241 cgggggcgtc ctccaccttg accgcgacca cctcctcgag ggtctcgagc accgccagga
     6301 ggcgcgtcgt gaagtcgccg tcgcccgacc accccgaggt gtcgagggtc ataggagata
     6361 ggggctaggg gctaggggct aagagctagg ggctaggagc taggggctag gggctaggag
     6421 ctaggggcta ggggctgagg aaccaggccg cctcttccct cttggctgct cccgtttcag
     6481 gatctccacg gttccgttgt tctccagcgc ctcgattgcc gccaccacct gctcgaggtc
     6541 cacgtcggga gcgtcgggcg agaggacgta ggcgaacttc tgatcatcgc tgcccaccac
     6601 gaggttgccg cattcggccc gcaccgcccg gtagatctcg aggatcgacc gcttgccgtc
     6661 gatgaagttc gccacctcgc tccgcgcaat gcccggcatc cggcccatcg gggccgccgg
     6721 ccggccggcg ccgcgctgtc cagtgccagg tgacggtgcc cggcgctgcg cctcgctcga
     6781 gtagaccggg aagagcctcc ggggcacgag gaggtcgcac tcgcgctccc tggcgccagg
     6841 agcggcctgg gggacggcgg caataccccg ggcagccgcg cgctcccggt acttcccctc
     6901 gagcagccgc gcgttcaagt cgcgagccgc ctggagggag ccgaccagcc tcctgacggc
     6961 ccctcccacc tcggcgtccc gtgccagcgc cagcaccgac tccacgccgc cctgcgcgcg
     7021 ttggaaggcg ccggccacct tcaattgggc ggcctcgtgc tgccgtgcga gtttctcgcc
     7081 atcccctgcc atgaggccgg ctgcctgccg cgccacctcg gcaatccacg cctgaccgtt
     7141 cgctgcggcc tcccaagcga ggtcccgcgc ctcggccggg ccggccgtgg agaggtagta
     7201 catggccgcg gccgccatga aggcgacccg cttcatctcg gtcgggtcgg cgtgctcggc
     7261 acggtcgccg ctgctgtggt agaagctgtc gttccagtag ttgaactgca tggcggggat
     7321 gccgccgttg ttgaagaccg agtggtcgct gcccgaggtg atcgcggcaa ccggcgtgag
     7381 gcggtagttg aaggtggcat tcgccccctg gggcgagcga atgttggtct ggtcgacgta
     7441 gcgcagcagg cttgcgatca ggtcgttgag gtagctgggt ctcgagtcgg gggtcagctt
     7501 cacgcggagg taggcgtcgg tggtcttcgg gttggcgccc gtcatgtcga agttcagcgc
     7561 cgcgtggagc cgctcctgca gctcggggtg cttgaacatg tactgcgtcg tccccgagat
     7621 ctcgggggcc cataggaagc ggatcgtgcg gcgcgggcgg ggcagcacgc cgtccctgat
     7681 cagtcgcgcg agcgtcctcc cgacctcgag ggtggtaccg accccggtgc agttgtcgtt
     7741 cgcgcccggc gtcgcgatcg tctcgaacgc gtgggcgacg aggatcagct cgcccgcggc
     7801 cgggtcgctg cccgggatcg ccgccgagac gacgttctgg cggccggggt aggtgcgcga
     7861 gcggacgtgc gcgcgcagca ccaccttctg gccgcgctcg acgaggtttc gcagctcgag
     7921 gaactgccgc agcgagagct ggaagccgaa gccggcacga tcggtcgcgg cgtacacgct
     7981 cgaccagccc acctggtcga gcgagtagct ggctggctcg ccctcgaggg ccgtcgtcgc
     8041 ggtgccgagc gcccctgcgg ccccccgctg gttcaccgcg gccccgaaca cgcctccgac
     8101 cggccccgct ccgagcacga tcttcccggc cacgtccttg ccctcgtagt cagcctgccg
     8161 gccggccccc acgtacacca gctcggcctc gacatccgag gtgcggctgc cgatcgcgag
     8221 cgaagcggga acggtcgtga tgctcgcgat cttcttccgc tcgggctgca ccatccagag
     8281 gtcaccctcc tcggcgtccc acgtgtcacg ccccgggaag aggtcgacct ggacgtccga
     8341 caggccggcc tcggccgccc tgtcgcgaat gtaggtggtt tcgaagaaca ggtccgtgta
     8401 ctccgaagcc tggcggtcgc ggttggcggc gagcatctgg acgtgcaggt gcgcacgctc
     8461 gcccgacacc tcgccgagga tcgggtcgcg gatcgcgggc ggcaccaggc agcggtcggg
     8521 cggcgcctgg gcgaggagcg cgaggccggc gagggtgaag acgaagaggg agacgaaggc
     8581 gaaacgtgga cgcatacggg aaatcaccta ttgatccggc cttccgcttc cggcgccgtg
     8641 cggctcagcg cccgaggccc ttcagcagcg cgagcgcctt ctcggcggca ctcttctcga
     8701 aggcagccag ctcggcggcc gacgggttct tcgcgatcag ccggctcacc tggtcgagcg
     8761 tctcgcgctc ggcttcgaga acggctggaa gccagcccgt cccggacgca ttgtcgaggc
     8821 gcgcgaggtc ggcgtactca cgccgctcgc gcgtcttcag cgggcgcagc acgtccttca
     8881 gccggaacgc ctcggtggtc agcgccgcct ccttcccctc ccagagcggc gcggggggcg
     8941 cgccggcggc cacccacagc ggcacggcga ccccggtcac cggctcgccc aggatcaccc
     9001 acatggtggc gagtcgtgga tcctgccctg gcttcacgcc gtggacgacc aggacgctgg
     9061 cggtgctgtt gcggttgatc gtgtggttgg tgtgaaccca gtacggccgg tcggcgggaa
     9121 actgcttcca ctcctcgcgc gcaaggttgg cgagcagcgg gtggccgagg tcgcgcgcca
     9181 gcgtctgcag cacgaaggca ggggtgaacg agcgtggagg tgccgcctcg agcagcgcgg
     9241 tctcgcggtc gaagcgaagg tagccggccc cctggtcctg cgcgccggtg cgcgagaagt
     9301 tggtgttggc catgtagccc tggggcgacg aggtcgcgtc gagccgcttg aacccgtggt
     9361 tgtggacctc gaagatcgac gccccgccct tcgcatcgat caccaggaag ttggcctgcg
     9421 cgccgaggtc ggggcccagg ttggccgcga agtaccgctc tgcgtcgtcc accctcgcgc
     9481 aggtgcgcag cacgtcggcc atcacgatcc cctcgaggtc tgcctgctcg ccgcctcgct
     9541 gcggcaggtt gtaggcaacc gagttggcga tggcgaggcc ggccgagttg agccctcccc
     9601 agaccatccg gccgtcggga tcgtcggcgt tgaccacgcc cagatagctg tacggcttgt
     9661 cgttgacgaa gacgaccttg ttcgacagcg tgtcggtgtc gcggttcttc cagaggatgg
     9721 ggcgcccatc cggggtcgag gaggcagaga agatggcggt cgtgcactcc tgcacgccgc
     9781 gatccgccag cgggaccgcg acggccagca acaggaggcc gaggatgatg gcgaggcagc
     9841 gggacatctt catgagcgcg gattgtaacc cggtgagggg ctggaggcgc ccgcagggtg
     9901 cgtgcgaaca tcccgcgcag ggcggggcgg ggcgggtttt acaagagcgt gcagaggtcg
     9961 tgtgggcgca cggccatcag ctcgcctcct cgcaggcgcg tctcgtcgaa ggtcacctgt
    10021 ccgaagccgt accgcgcaat gcaggcagcg gtcccggcgg cgtgcgcggt cagcaggtcg
    10081 atcgtcgagt cgccgacgag caccgtctcg cccggcggaa cgcctgcggc cgcggagagg
    10141 tgcagcaggc cggcgggggc cggcttgcgg gggaaggggc cgtcgcctcc gacgacctcg
    10201 gcgaagaacg gcctcaggcc gagcgcgtcg agcagcttca ggctcgcctc ggtcggcttg
    10261 ttggtcagca ccgcgaggcg tgcgcggcgg ccgagcacgc cgagcgcctc ggggatcccc
    10321 tcgtagggcc tggtctcgcc ggggaggagg ccgtcgtaga tctcgaggaa gcgggaaagc
    10381 gcgtcggccc cgtcgccctc gagattggct gcggccatgg cccgctccac gagcagccgc
    10441 gcgccctccc ccaccatccc cgccaccgcc cgcctcgaca gcggcccggc cccacgctcg
    10501 gcgagcatcg cgttgacggc ggcggcaagg tcgccaatcg agtcgatcag ggtgccgtcg
    10561 agatcgaaga ccaccagccc gaatcgtggc atcgcgtcaa ccggcctggt aggtggcgta
    10621 ggcgttgggc gtctcccaga cgcgcacgcc caccacgtcg aacccctgtt cccgggtata
    10681 gttgaagatc agctgggcga tcagttcgac ggtggggttc gagtcgacca ggaacatcgg
    10741 ctcgccaagc tggcgcagcg gctcgaccag cgggtcgtcg cggcgcagga tcatcttgtg
    10801 atccagctcg cggtcgatcc accccttcac cgtgcgcttg atgtcgccga aatcggcgac
    10861 catgttgcgg gggtcgagcg aggcggtgcg cacctcgatc tccgcgacgg cgttgtggcc
    10921 gtgggggtgc ttgcagaccc cgtcgtaatc gagcagacgg tggccgtagc agaactcgat
    10981 acgcttggtg acggagtaca tgggggaatt gtacaaacgg agaagggaaa acggaaaacc
    11041 gggagcggca gacgaggaac ggggcgttca tgaacgcgat agcggtcctg tgctccggcg
    11101 ggctcgatag cgccgtactg ctggcccgcg aggcccgcga gcgcccggta ctccccctct
    11161 acgtccggtc gggccttgcc tgggaggccg aggagcggcg cgcgctcgag cacttcctgg
    11221 cctcgctcgc ctcctcggcg gccgtcctgc cgctggcgac cgtgcaggtg gacgtccagg
    11281 acctctaccc gggctcgcac tgggcccttc gcggcacgcc gcccgcctac gacaccccgg
    11341 acgaggacgt gtaccttccc gggcggaacc tgctgctgct gtcgaaggcc gcggtgctgt
    11401 gcgcgctgcg aggcatcaac cggatcgcgg tcgggccgct ggccggcaac ccgttccccg
    11461 acgcgacgcc ggccttcttc gagtcgatcg gccgggcggc ctcgctcggc ctcgaccacg
    11521 agatcgaggt cgtcgccccc ttctcccgga tgcgcaagga agacgtgatc aggctgggcg
    11581 tcgagctggg cgtggcgttc gagctgacgc tgtcgtgcat gaatccgtcg ggtgggatgc
    11641 actgcggcct ctgcagcaag tgccgggagc ggcgcgacgc gttcgcggcc gcgggggtgg
    11701 aggatccgac caagtaccgc gcgaggtccc cgcgggaaat cgggtgaggt gacttccggg
    11761 tccctacaat cctgccaccg tgatcgttcc actgccgctg cgcagccgga ggagcttgcc
    11821 gccgccattg agctgacgct tcaactcgcg gcgcgagcgc tcgtcgcgga cctcctcgcc
    11881 cgccggctcc ggctcccggt ccgcctcctc cccgatcccc tcgagacgca cccggccgtc
    11941 ggcggtgctg gcatccaggt tcgcggcgaa ccccggcggg atctcgagac ggacgccgcc
    12001 atctccagtc cggacctccc aggggtcagc catctcgctc ccctcctcgg cccgaaccac
    12061 caccccgccg tcgccggtca ccaccttcac cgactgcagc ttgccggcca ggctgacggt
    12121 gccgtcgccg gtgtcgacat cgacccaccc cgacacctgc tcgaggcgga ccgagccgtc
    12181 gcccgtgtgg atgcgaagcc tgccggccag gtcgtaaccg cggacgctcc cgtcggcggt
    12241 gtcgaggtcg aggtcgcctt cgacccgctc gacggttatc gacccgtcgc cgctgcgcac
    12301 cgcgatcttg gcccggcggg gcacgagggc aatcagcctg gcggtcggcg actggcgcag
    12361 gaagccgccg gtctccccgg ccggcttttt cacggtgagc gtgatgaggt ggccggcctg
    12421 ctcggcccgc acctcgatcg cctcggccat ttgctggttc ggcccgcgct tctcgacctc
    12481 cacctgcacc gcggcctggt cccacgagcg gatctcgacg ctgccgtcga aagtggccaa
    12541 ggtgacgtcc ggaacgccct gcggcacctc gaagcgcttt tcctcgcgcg cggtgtactc
    12601 gctgctgtcc accgacacca tgcagccggc caacgtgatc gacgagcaca gggcgaagga
    12661 cgcggcgagc ggacgaaggg cgtgtcggtt catggcggac ctctgttggc ctatacgggg
    12721 cgcacctgcc gaaagttcgc cccggtcagc ggggtgccgg ccgccagtcg ggatgggcgt
    12781 gcagcttccg gccctcgagc gggaggggcg cggcggccgg gcgaaccctg gcctttcccc
    12841 gccggggccg ggggcgaaag atggcccgat cgaggcgcga cagcaggcgc gagagcccgt
    12901 tctcgtcggg cgcggcgatc accagcacac cggcggtcgc cgacggcgga aaccggcgat
    12961 cgtccaggta atcgcggtcg agcgtcagca gcgtgcggcg cagcttcgcg gcgagccgga
    13021 agtgctccgt gtcgggctgc cgccgcaggt catcgtgctc ggtcacgaac agcacgtccc
    13081 accgcagccg gactcgcatg aaggtcacga gcccggccgg cacgttggcg tcggcgtaca
    13141 ttcgcggctg ccccgccagg cgctcggcca ccgccgacag ctcggaggca agcgacttca
    13201 tagccctcac ccatggtagc ccggagacca ccgccgggaa tcacggcgcc ggcggcctcg
    13261 cggcccggcc tcgctgcaag cgcagcggca gggatacgac cagggccctt gcaaccgccc
    13321 cgcccagcgc ccacaatacg ccgcatgacc atcgagcagg ttctgatcgc cgccgccgtc
    13381 atcgtgctcg cgggcctggc gctgatcggc ctctggatct actggaaggg tcgccccttc
    13441 gcccagggcg acgtgttccg cgccagccgc ctgagccgag gcaaccgcct cttccccacg
    13501 caggtcctga tcaccccgca cagcgtggtc caccatacgc cgcagtgggt aggcaagctc
    13561 gaggagacga tccacctggc gcacgtggcg tcggtccgga tcgatacgaa cctgatgttc
    13621 tcggacgtcg cgatcgaaac cagcggcggc accaacccgg tcgtctgcca cggtcacagc
    13681 aagaaggacg cggtgcggat gaaggccctc atcgaggagt accagtcggc ttactaccgc
    13741 gccccctcgc aggcgccgcc cgccgcgcca ccgcccgccg cgacaccgcc cggaactgtt
    13801 gcctcgccgc cgtcgagccg atagtcagag agccggcacg ggccggctca ctgacgtggc
    13861 atccgacttt ggcagcttcc ccgcacggcg cgataccttc cccagggccc ggcccacgcc
    13921 caccctgggc gacctcccca cccgcttccg cacgctggtc cagtcgccgc tgcgcgccgg
    13981 catcctccgc ttcatgcact cgcgtccgaa cgacgtgttc gacgtcgagt cgctgatgca
    14041 ggtgttcggg cggatgcgcc tcgacgtgga caactgcgtc ggcgagctgg cgaacttcgg
    14101 ggtcgtgcgg cggctgcccg gacagccgcc gcggttcatc gccgggcggc ccgagcagga
    14161 ggcggtggcc aacctgctcg acgacttcct cgagcggcgc gccaccgtca gcaccgagga
    14221 ccagtcgccg tcggtgcagc gcttccggga gatgatcggc cgcgacgaga agatgctgat
    14281 cgtcttcgag tggatccgca cggccgccaa gtccgacatc gccgtcctca tcctcggccc
    14341 caccggatcg ggcaaggaag tggtggcgag gatgatccac gagctgagcc gccggggcgc
    14401 cgggcggttc caggcggtca actgcgccgc gctgcccgac acgctgttcg agtcggagat
    14461 cttcggccac gagagggggg cctacaccgg cgcccacgac cgcaagccgg ggcgcctcga
    14521 gctggcgaac ggggggacgc tgttcctcga cgagatcggc gacctctcga tcgtctcgca
    14581 ggcgaagctg ctgcgcgcac tggaggatcg cgggttcgag cggctcggcg gcaaccgcac
    14641 gatcgaggtc gacttccgcc tgatttccgc caccaatcgc ccgctcgagc agttcgtgcg
    14701 cgaggggcgg ttccgcgagg acctgtacta ccgcgtgaac gccttcgcca tccgactccc
    14761 gtcgctgcgc gagcggcagg tggacatccc ggtgctggcg cagaggttcc tcgcccgcta
    14821 ctgcgccggc aacggcctgc cggtggacgc gaaaaccttc tcgcgcgagg cgctcgacgc
    14881 actgggcgcg tacccgtggc cgggaaacat ccgggagctg gaaagcaccg tgtcgcgggc
    14941 ggccctctcg gcaccgggcc gcgtcatccg cggaagcgac gtcgacttcc tgcacgcggg
    15001 aaccgaggcg atcgaccctg ccactgtccg cctcgcctcg ctccgcgaga tcgagcgcgc
    15061 gcacatcgtc aaggtgctcg aggcggtggg gtggaacaag aaggaagccg cgaccgtgct
    15121 cgacatcagc cggggcaccc tctaccgcaa gatcacggag tacgggctcg aacgccaggc
    15181 ccgggcgctg ttgtccaaga acagggcaaa cctccggact gcctagcatt ttccgagcaa
    15241 ataagggcgc tttcccgatg tcccgaaacg ggacgctgac gaataccgcc taagccattc
    15301 cattcaggtc acttagcagt cgcacggggc ccgtgcctgt tccaccccgg cacaccttct
    15361 cgctccgcgc ccggcctcgc cgcgcccgaa ccgaagaccg gaagtcccag caaacacctc
    15421 tcccggttga agttacgggg caggcctcgc tgtccaagcc caaccggcag tggaattgcc
    15481 tcatcagtgg gcagtggaga ccatgtcgaa gaacctcggc ggtgggattc gggacgcggt
    15541 ggcaggggcc agcaagtggt ggaggaacct gctcgcgccc gcggcccttt acagggccgg
    15601 cggggcggcg gcggcggcgg cggcgtgggc gttcgcagag ggacggccgc tgtatggctt
    15661 gtgcgcgctg gggctggcgc tggcggcctc gcggcgctgc cgcgtgcagt cgcggcagct
    15721 ggccgagctg gcgcgccgcg agcggctgcg ctcggacgtc aacctggcga cgatcgaggc
    15781 gctggcgctg gccatcgacg ccaaggacaa gacgacctct tcgcacatcc ggcgtgtcca
    15841 gcggttctct gcggccctcg ccgagcgaat ggagatggat ccggacgacg tgctgggtgt
    15901 caccaccgcg gcgctgctgc acgacatcgg caagctggcc gtgcccgagc acatcctctc
    15961 caagccgggg ccgctgaccg acgaggagtt ccagaagatc cacatccacc cgcaggtggg
    16021 gttcgagatt gtcgagcacg tgccgtttcc ctacccggtg gcccagctgg tgctctgcca
    16081 tcatgagcgg tgggatggga agggctaccc gctcgggctt cggggcgacg acatcccgct
    16141 cggcgcgcgc atcctggcgg tggccgacta cttcgactcg atgatgcgcg accggcccta
    16201 tcacaagggc gtctcgcgcg agctggcggt ggagatgctg cggcaggaag ccgggcgcgc
    16261 cctcgacccg cgcctggtgc aggtgttcct cgatctgctg ccggggctgc cggacgtgca
    16321 ggagccccgc tccgtgccgc tgcccgtggt cccgcgaacg ggcggccgcc acctgttcga
    16381 gagcggcgag caatcggcct tcgcgaacat cgcccgcgcg caccaggaaa tctacgccct
    16441 ctacgacatg gcgcaggcgc tcgggtcgag cctcggggtg gccgacacga tgtcgctgct
    16501 cgcggcaaag ctctcggcgc tggtgccctt ctcggcgtgc gcgctgttcg ttcgcgagga
    16561 tgacgagacg ctccgttgcc ggttcgccag cggcgtggac gccgaccacc tgcggcgcgt
    16621 gtcggtgcgg gtggggagcg gactggccgg ctgggtgacg cgaaaccgcc ggtcgctggt
    16681 gaacgcgcgg ccggccgccg acttcgagac cgccgacctg gccccggcgc cgccctcgct
    16741 gcgcgccgcg ctggtgtgcc cgctcatctg cgccggagag gtcatcggga cgttgtcggt
    16801 ctaccacacc gacccggact tctaccgcga cgagcaccgc cgcctgctgg agcggatctc
    16861 cgagcaggcc gcggccgcca tctcgaactc gatcgtgttc gagaagaccc gcgaggcgtc
    16921 gctgagcgac ccgctgaccg gcctccccaa cacccggttc atgttcaact acctggggcg
    16981 cgagctgtcg cgcgcgcagc ggctccgcac ccaggtgtcg atcctcgtcc tcgacctcga
    17041 ccggttcaag aacatcaacg acacttacgg ccaccacgtc ggcgaccgcg ccctgcgcga
    17101 ggtcgcccgc gtgctccgcg agggcattcg cccctacgac atctgcgtcc gctacgccgg
    17161 cgacgagttc atcgtggtgc tggccgactg cgggttcgac gaggccgagg ccaagcgcgc
    17221 cgagctgcag caggccgtcg acgagctggt gctccagctg gatggcggcg aaacggccag
    17281 gctcagcgtc agcatcggcg cagcggtctt cccgcaggac ggcgagacct acgaggcgct
    17341 gctcgcgacc gccgacggcc ggatgtaccg cgacaagagg ggacggaagc gcgcggtgag
    17401 ccggtgcgcc gtgcgcgagg ccgcctcaat cgactcaccc gaggcctccc cggcggccgt
    17461 cgagaaggtg cgccccttcg ccaaggtcgg ctgaacgccg cccgtggccg cgacacggta
    17521 agatcgaagg gtgcccctgc tgacgctgcc tatttcctcg agccgccggg caaccccccg
    17581 gaacctggca gtccgcctcg acctcggcgg gcgcccgttc gacttctcgg caggccagta
    17641 cgtgctgctc ggccaggcgg gtggtgccga ccgttgcccg tactcgatag cgtcggcacc
    17701 cagcgaagtc gccgcgagcg gcgcgctcga gttcctgatc caggtggacg agcacggcag
    17761 cccgggcccg cacctgccct ccctcgcccc cggccgatgc ctggcagtcg aaggacccgc
    17821 cggcagcttc acgcttcccg ccggcctgcc agacgccgac ttcctgttcg tggccggcgg
    17881 gaccgggatt gcgccgatcc gttcgatgct cgtcgaggtg ctccggacgc tgcccagcgc
    17941 gcgcgtcacg ctggtgcaag ccgcgcgcag ccccgaggag ctgtcgtacg gcgacgagtt
    18001 caggtcgctg gcgcgggagg ggcggatcgg gctggtggag agcgtcacgc gcgaggcgcc
    18061 gcccgggtgg cagggcgtcc gcggccgcat cgccgcggcg cacctcgccc cgctcgtcgg
    18121 cggcgcgcgc actgtcgcct tcgtgtgcgg ccccgactcg ctcgtcgaag aggtgccccg
    18181 ggtgctcggc gccctcggcg tctcgaacac gaagaccgag cggtgggctg ggtgaagaca
    18241 gggctcccgc cttcgccaag cgctcgctac gctcgcggct acggcggggc aggcgcggct
    18301 acggcgcgtc tcacttcgga ttcaccacca gcgtcgactt gacatccttc acgcccggga
    18361 tccggcgggc gatggcgacg gcctgctgct cctcctcggg cgtccttgcg acccccgaga
    18421 gggtcaccac ccccatcgtc gtgtccacgt cgatccggag cccgccgacg ttgggatcgt
    18481 tgagcagggc cgtcttgacg cgcgttg
//
LOCUS       DAOPQX010000029        18042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3732_length_18042_cov_4.674654, whole genome shotgun sequence.
ACCESSION   DAOPQX010000029 DAOPQX010000000
VERSION     DAOPQX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 18042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18042
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3732_length_18042_cov_4.674654"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PKK95_02905"
     CDS             <1..799
                     /locus_tag="PKK95_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOC17186.1"
                     /translation="SYRNHRKVAVIDGRIGYSGGLNMTERHLTGPPGFTGWRDTHARV
                     IGEAVPILQGVFATMWHNTTGERLFDEAYFPETGDAGRRLPIQVVSEGPDSRWEAIRH
                     SYLAMIAVAQRRVFLQSPFLIPDTSLGEALKTAALAGLDVRLMIAPRGAELSPAYRAG
                     MTYALDMAEAGVQVWLYEGDYFHAKTASVDSMVCSIGSANMDIRSFSINYETNLVIYD
                     PFVAQEVEADFMADLRRCVRFSPEAYRARRVTSRFVDSFLRLFSPLL"
     gene            891..2732
                     /locus_tag="PKK95_02910"
     CDS             891..2732
                     /locus_tag="PKK95_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOC17187.1"
                     /translation="MRSFALLLLFASSCFFAPSAGFAQPSGGPYGPIERVYEIPKAPH
                     VYYVAPDGKTDAPGTTLDQPTTIEAAIARVVTGDVIVLRGGVYRTGGLELNQGITLQP
                     YAGERPILKGTRVADQWQPLRSNLWRTQWKTLFPARPLGWWQRAREGMRTPVHRFNND
                     MVFVDGRMLKSAGWEGEVGPDSYFIDYDSGHVYIGVDPKGHEVEITAFDFALIRRARP
                     VHGKESDRKGPTIRGITFTQYAFRALEIEGKTGAASPSEEPTNEPVGLSDPATYGKEV
                     TGTTLENVTVTFCSRVAGYFRGDRLTIRNSLFSDTSTEGVYVIGSSDVLLEKNIFRRN
                     NVEQLTGYYPAAVKIFNQSHRVTCRDNLVVDNPYSNGIWYDVGNRDGVFVNNWVEGAI
                     DGFFFEISKGVTVAGNLFVRCDKGVRILNAAGARVYNNTFVDAPASFERNQRVASGDL
                     FGWHAATGPGLEERDGHVFVNNLLVASESYTRPLLRFEQPPQMCQKLPRPQAAEVDGN
                     VYVRPGGRESQPLVVWSPAATETCMATAASLDEFRAVAKGVEARGRQVERSPASIFKG
                     PDVGRYELLGDLPAAGATCPLPGDVRALLGWSEQEAARTAGAYPARR"
     gene            complement(2758..5601)
                     /locus_tag="PKK95_02915"
     CDS             complement(2758..5601)
                     /locus_tag="PKK95_02915"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOC17188.1"
                     /translation="MTIRTRRPAAGLPGLLALLLVLFAPGSLAGQDKPGWESVALDAP
                     VPVDPRITIGYLPNGLRYYIRTNERPVQRAELRLVVKVGSVVEDEDQRGVAHVLEHMA
                     FNGSKHFAKQDLITFMESIGMRLGPGVNANTSFDETIYQLHVPTTNEEAMNKAFLFFA
                     DVAHALTLDTESIEKERGVVLEEWRQGRGAGARMQDQQVPILLKGSRYADRLPIGTRE
                     SLQTFKPDALRRFYKDWYRPDLMAVIAVGDFDKRDIEALIKRHFTPIPGAIAARPRPS
                     FEVPDHEETLFAIATDREAPITSVGLYNKLPLRDQTTVGAYRQQQVERLYTTMLNSRL
                     AELALRPDPPFLRAATDRGIFVQTKETASLVALVKEDGIERGLEALVTESSRAARFGF
                     TPAELEREKRNILRAYEGAFDEREKEESADLADEYIRNFVEREPIPGITYEFGLVQRF
                     VPHFTLEEVNKVAKDWAGGSRVVLVNAPEKPGLTVPDASRLAAVMKSALEREIEAYAD
                     AGGATALMETPPKSGAVVESTNTLAFNVTEWKLSNGVRVVLKPTDFRQDEVVFRATSP
                     GGTSLASDAQYVPAITAAEVVGASGVGRFDAIQLRNVLSGKAASVMPFINPTSEGLAG
                     GASPKDLETLFQLIYLSFTEPRADENVFRVMQDQMRAMLASREASPEWALRRTLQAAL
                     TQDHPRARPLTAEMVGQMDLQQSLAFYKDRFRDASDFTFVFAGSFEPQAIKPLVEQYL
                     GALPGAGRKESWRDEGVTPPAGVVEKEVRKGIEPKSQVTIVFTGPMNAPASERVALDA
                     LALALENRLRLTVREALQGSYGVEVRATAQPIPSPRYSVTIDFGCDPKRADELVKSVF
                     QDIGALREKGPTDREVSDAREALLRRHETSLARNPSLVGEISDRLENGEDLSTYFGLP
                     REYRQLTPRMVQDAARRYLDPGRYVRVTLYPER"
     gene            5728..6177
                     /gene="nrfH"
                     /locus_tag="PKK95_02920"
     CDS             5728..6177
                     /gene="nrfH"
                     /locus_tag="PKK95_02920"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOC17189.1"
                     /translation="MRYLPALVVGASLGVIAGIGGYTFVYAKGASYLTNDPAACANCH
                     VMNEQYDAWLKSSHHAVAACNDCHTPHALVPKYTTKGLNGFWHSYAFTTGDFPEPIQI
                     TGRNRRVTEGACRECHADMVHAIEGVPASADDSVSCIRCHRSVGHLH"
     gene            6234..7604
                     /locus_tag="PKK95_02925"
     CDS             6234..7604
                     /locus_tag="PKK95_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOC17190.1"
                     /translation="MKLKPPALVLLTAGVTMLVVAGLTALLMNIREHKEEARNPFFRV
                     VTLTDDTEDPEVWGKNFPLQHDLYRRTVDQVRTKHGGSEAQPRTPTDADPRSIVAQSR
                     LEEDPRLKTMWNGYAFAVDFREERGHAYMLDDQTFTGRQLVTPQPGTCLNCHASMYVT
                     YKKLGGGDIVKGFEAINRMPYAEARKAVKHPVACIDCHDPDTMQLRVTRPAFMEGIRA
                     LKASQGVANYDVNTMATRQEMRAFVCGQCHVEYYFKGAEKRLVYPWAKGIRVENILDY
                     YEEVGHKDWTHADSGADALKAQHPEFEMWNQGVHARSGVACADCHMPYLRQGALKISD
                     HHVRSPLLNLSRACQTCHRWTEEELKARAETIQDRTFQTRNTAMDALVALIGDIKAAR
                     EAGVPEPQLQEARRAQRRAQFMVDFVEAENSTGFHAGQEAVRILGQAIDTARQGQLSL
                     RPPVGR"
     gene            complement(7635..8549)
                     /locus_tag="PKK95_02930"
     CDS             complement(7635..8549)
                     /locus_tag="PKK95_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17191.1"
                     /translation="MTTPPDSPRRAAIALLLWAWHTVLALVVAFPFFTWMSRETGVAP
                     GTDPLLDGIQFGTLFELLQDSAGSLRGVPAALISAIGLALLSNALLGGGLFALAAGRA
                     AAEGRLLSRFFEAAGRFFLRSVGLIALSAVAVLAVGGTAAALAGAVLEPILARAGEPG
                     DVAQAALTMALVACVAAYFVLALDYARVWMVRHDARGVVRTWLRGAWFVLRHPAKAAM
                     PGVVYGLGIAAALALGAWIGGAPGGRTWVVIAGVLAAQQALLYLRVLLRVNMVVAEGR
                     AAAGWEPEPDAAPAPAPASLPSSSAGIQ"
     gene            complement(8552..10657)
                     /locus_tag="PKK95_02935"
     CDS             complement(8552..10657)
                     /locus_tag="PKK95_02935"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOC17192.1"
                     /translation="MAALPHPLFPLVLSAALLVLAPPGESSAGPLPERSDRIVRYDIA
                     VRLDPEKKQLEGRETITWRNPSGDPVPDLWFHLYLNAFRNTKSTFFRESGGRLRNDRA
                     TENWGWTEITSLKLADGTDLMPAIRFEHPDDDNADDRTVARVVLPRPVEPNGAVTIEL
                     AFKAQLPEVYARSGYKGDFFLVGQWFPKLGVYEPAGRRGRASGGWNCHQYHANSEFYA
                     DFGEYRVRITLPSRFVVGATGSRVASEEASGQTTHVFEQADVHDFAWTASPRYLVVND
                     TFSAGKDVSDAEYRATAARLGRPLDEVRLSDVAITLLLQPEHRPQAARYIRAAKAGLK
                     WYGLWYGRYPYRTLTVVDPPAEAGGAGGMEYPTFITGGTFWLGNFWPLDGVRAAEMVT
                     VHEFGHQFWYGMVANNEFEEAWLDEGINTYSTGLAMDAEYGDASSYASLPGMRIGDVD
                     VLRVTTTPDQKDVIMKPAWGYAGDYSTYAYQKPATALRTLQGLLGDETMARVMRTYHE
                     RWRFRYPSSADFFATASEVAGRDLSWFFHQAFETGHVLDYGVTAVSSVEAKAARGVFE
                     PGGGQKAEGGTEKEKEEKSLAQDAAPEPRGEAKMYESRVMVTRHGEFVFPVTLAVKFE
                     GKPVERLQWDGRDRWKRFTFTRPERLEWAAVDPDRKVWLDANWLDNARRVSPDKRVAA
                     SWTSRFLFGVQALLMFLGL"
     gene            complement(10794..11411)
                     /locus_tag="PKK95_02940"
     CDS             complement(10794..11411)
                     /locus_tag="PKK95_02940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC17193.1"
                     /translation="MSSKFLAALCGALVLSVGVASAQAAPAQAKEKKLTPGTYAHFVT
                     TEGEFTVRLFDKEAPKTVANFAGLAEGTIDPATGKPGKSKPFYNGLTFHRIINGFMIQ
                     GGDPLGNGTGGPGYRFADEFHPKLRHSKAGILSMANAGPNTNGSQFFITLGPTPHLDN
                     KHSVFGEVVEGMDVVKKIGAVRVLPGDRPAKPVIMKTVTIERVTQ"
     gene            11542..13752
                     /gene="glgB"
                     /locus_tag="PKK95_02945"
     CDS             11542..13752
                     /gene="glgB"
                     /locus_tag="PKK95_02945"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOC17194.1"
                     /translation="MSHDDLLQAAAALEHGTHPDPFAVLGPHPRAGGRPGVVVRAFSP
                     DAVAAELLPATLPPQPMARVGSSGLFHAAIEGWEGEPLAFDYRVRFTWPDGSASEVVD
                     AYRFGRVLTDEDLYLIAEGTQYRAQDKLGARPLQFGIVSGVHFAVWAPNAERVSVVGD
                     FNRWDGRRHPMRRLAPQGVWEIFIPGLANGEKYKFEIRARGGAVFQKTDPYARRLEVP
                     PKSASIVWHPSPYPWQDREWMERRERENGWLERPVSIYEVHLGSWARGADGQRFLSYR
                     ELAERLVPYVRDAGFTHIELLPVMEHPFAGSWGYQVTGFFAPTSRFGVPEDFKAFVDA
                     CHLAGLGVLLDWVPGHFPKDAHALARFDGTALYEHEDPRLGEHQDWGTLIFNYGRHEV
                     RSFLLSNALFWLEEFHVDGLRVDAVASMLYLDYSRREGQWIPNRYGGRENLEAIDFLR
                     ELNSLTHGKHPGTITAAEESTAWPGVSRPVHLGGLGFTYKWNMGWMHDMLSYMSHDPI
                     HRRWAHNQITFSMLYAYHENFILPFSHDEVVHGKGALIGKMPGDSWQQAANLRALYGY
                     MFGHPGKKLLFMGDEFGQRAEWNHDRGLEWEYLDVFPHGGVLALVRDLNALYRSQPAL
                     FEQDYDPAGFQWIDCNDNENSVVTFLRRARDPRDFLVFVLNLTPVPRQGYVVGVPEPG
                     SYRELLNTDSELYGGSNLGNGGFVASEPVAAHGFSDRLRLVLPPLACLVLKIER"
     gene            13916..14965
                     /locus_tag="PKK95_02950"
     CDS             13916..14965
                     /locus_tag="PKK95_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC17195.1"
                     /translation="MGAAADASLSGPLEETITLGQAKRLIQCMAHEHSFLLLAPPGVG
                     KSETVYQAAEEAGLPCRSLLGTQIAPEDVSGVPRIVGERSVFCPPRVLLPEKPEPFCL
                     FLDELPACAPDVQKAFYSLLLERRIGEFALPPGTWVVAAGNRVQDRALVRSLSSALVN
                     RVTILNVRVDVGEWMAWARGAGVREEVRTFISYMPEALMRPVPQEPVPFSTPRSWTLF
                     ARALDLAEAAGELTRAARRALAFGRLSAEDAAVFCALAEESIGAMQPIAYYFAHPEAL
                     PSGDTARWFVLNCIRQNVKDGTLHDLAPQEVNRFLRSLPHEHQLTLLTDLVDRWGALG
                     ADEAMFDLLKEVAHL"
     gene            14962..16200
                     /locus_tag="PKK95_02955"
     CDS             14962..16200
                     /locus_tag="PKK95_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HOC17196.1"
                     /translation="MSAPSSDRALADRIERALRMVTVPFPHLAGLVASARVTIDERVP
                     TIGVFASGRLSANPAFVRGLKENELVFVLAHEMLHLALRTHDRARGADRLEFNYAHDY
                     IINDILRTELGAPVPAGGLDMPGARDRSAEEIVLEMRRNQQGQSKTRVWQGEATTAGR
                     VFGARGRGAGTLEAGDVLDLSAEADLYPEDAAESGERAEQVKEMAAKALGLARAMGLA
                     RGVRGTDAGAASQTVLALRGIYRTPWKLALQRWIESVAPGDRTFSRPSRREISQPDVV
                     LPGRRREGWILNIVLDTSGSMTDEIPRALGTLGEFCDAAGVDQVRLVQCDAEVTSDEL
                     LAPYELAERRIEGYGGSDLSPALQHLAGDQHVRAVVVITDGEIAYPAEAMPYELLWLV
                     PPGVRAFRPPYGMVVVMQQA"
     gene            16222..16608
                     /locus_tag="PKK95_02960"
     CDS             16222..16608
                     /locus_tag="PKK95_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HOC17197.1"
                     /translation="MKAIQELVAYFERRGRLTPIQVLKLLDQGLLAGDAPANMLDLCD
                     QIGATYYFRLTGQATGMVWGTDVYTGDSSLAAAAVHAGVVKDGEAGVVKVTVAPPLSR
                     YQGSSRYGVTSHDFGHYGTAYRLSRV"
     gene            complement(16624..17967)
                     /locus_tag="PKK95_02965"
     CDS             complement(16624..17967)
                     /locus_tag="PKK95_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOC17198.1"
                     /translation="MRTSGFVSTLVLGLAASVAAVLPLDAQRPSISVAEHQARRQALM
                     EKVQDGLIVFFAEVDAPAGSRFVQDNDFYYFTGSNDAGAILVMSPRAKASYLFLQQKA
                     AREELMDGRNLLGDPKGAEKLGVTAIHPVSFFEEFLARTAAAAGPTLHLRLSPRDLLD
                     GARSETALFAGRRARTPYNDQSSLDQYRVARLRERYPQAVLADVAPLIDAMRAIKTPG
                     EIEILRRNGRVSAEAVKRAMLAARPGAYEYEAEAAAMHEVLRSGGLGAAYAPIVGSGP
                     NTCILHYADNGRRIEEGDLVLMDFGGQMDHLAMDITRTWPASGKFTPEQRAVYERVLA
                     VLEACIEAYRPGATVDDVRRHVAEVMKRKGLEQGPELRGGFGHGVGLSTHDVPLGPVL
                     REGMVFAIEPGLYFPDKNLGIRIEDTVLITKDGCEVLTRDVPKKVEEIEALLASR"
BASE COUNT         2714 a         6259 c         6345 g         2724 t
ORIGIN      
        1 cagctatcga aaccaccgga aggttgccgt catcgacggg cggatcgggt actcgggcgg
       61 cttgaacatg accgagaggc acctgacggg tccgccgggg ttcaccggct ggcgcgacac
      121 gcacgcgcgc gtgatcgggg aggccgtgcc gatcctgcag ggtgtcttcg ccacgatgtg
      181 gcacaacacc accggcgagc ggctgttcga cgaggcgtac ttccccgaaa cgggagacgc
      241 cgggcggcgg ctgccgatcc aggtggtgag cgaggggccc gactcgcggt gggaggccat
      301 ccgccactcg tacctggcga tgatcgcggt cgcgcagcgg cgcgtcttcc tgcagtcgcc
      361 gttcctgatc ccggacacca gcctgggcga agcgttgaaa acggccgccc tcgccgggct
      421 cgacgtgagg ctgatgattg ccccgcgggg cgcggagctg tcgccggcct accgggcggg
      481 gatgacctac gcgctcgaca tggcagaggc cggcgtgcag gtgtggctgt acgagggcga
      541 ctacttccac gcgaagacgg cctcggtcga ctcgatggtg tgctcgatcg gctcggccaa
      601 catggacatc cggagcttct cgatcaacta cgagaccaac ctcgtcatct acgacccgtt
      661 cgtggcgcag gaggtggagg ccgacttcat ggccgacctg cggcgctgcg tccgcttctc
      721 tcccgaggcg taccgcgcac gccgtgtcac ctcgcggttc gtcgactcct tcctgaggct
      781 cttctcgccc ctgctctagg agccacccag tcttgagccc cgccgggaga gcctttatag
      841 tgtgcctggc tcttccggcg cggtcggcaa cccctctgaa aggacacacg atgcgctcgt
      901 tcgcccttct cctgcttttc gcttcgtcgt gcttcttcgc gccctcggcc ggtttcgcgc
      961 agccctccgg cgggccgtac gggccgatcg agcgggtcta cgagatcccg aaggccccgc
     1021 acgtctatta cgtggcgccc gacggaaaga ccgacgcgcc cggcaccacg ctcgaccagc
     1081 cgacgacgat cgaggccgcc atcgcgcgcg tggtgacggg cgatgtcatc gtcctgcgcg
     1141 gcggggtgta ccgcacaggc ggccttgagc tgaaccaggg catcacgctg cagccgtacg
     1201 ccggcgagcg gccgatcctg aagggaaccc gggtggcgga tcagtggcag ccgctgcgca
     1261 gcaacctgtg gcgcacgcag tggaagacgc tgttcccggc caggccgctc ggctggtggc
     1321 agcgcgcccg cgaggggatg cggacgccgg tgcaccgctt caacaacgac atggtcttcg
     1381 tggacggccg catgctgaag tcagccggct gggaaggcga ggtgggcccc gactcgtact
     1441 tcatcgacta cgactcgggc cacgtgtaca tcggcgtcga tcccaagggc cacgaggtcg
     1501 agatcacggc cttcgatttc gcgctcatcc gccgggcgcg cccggtccac gggaaggagt
     1561 cagatcgcaa ggggccgaca atccggggca tcaccttcac gcagtacgcc ttccgcgcgc
     1621 tcgagatcga agggaagacc ggcgccgcgt cgccctccga ggagccgacc aacgagccgg
     1681 tgggactctc cgaccccgcg acctacggca aggaggtcac cgggacgacg ctcgagaacg
     1741 tgaccgtcac gttctgctcg cgggtcgccg ggtacttccg cggcgaccgg ctgacgatcc
     1801 gcaactcgct cttcagcgac acctcgaccg agggggtgta cgtcatcggc tcgtccgacg
     1861 tgctcctcga gaagaacatc ttccggcgga acaacgtcga gcagctgacc ggctactacc
     1921 cggcggccgt caagatcttc aaccagtcgc accgcgtgac ctgccgcgac aacctcgtgg
     1981 tggacaaccc gtactcgaac gggatctggt acgacgtcgg gaaccgcgac ggggtgttcg
     2041 tcaacaactg ggtcgagggg gcgatcgacg gcttcttctt cgagatctcg aagggcgtga
     2101 cggtggccgg caacctgttc gtgcggtgcg acaagggagt gcggatcctg aacgccgccg
     2161 gggcgcgggt ctacaacaac accttcgtgg acgcgccggc atcgttcgag cgcaaccagc
     2221 gcgtggcgag cggcgacctg ttcggctggc acgccgccac ggggccgggg ctggaagagc
     2281 gcgacggcca cgtcttcgtg aacaacctgc tggtggcgag cgagtcgtac acgcgtccgc
     2341 tgctgcgctt cgagcagccg cctcagatgt gccagaagct gccccgcccg caggcggccg
     2401 aggtggacgg caacgtgtac gtgcgcccgg gcgggcggga gtcgcagccc ctcgtggtgt
     2461 ggagcccggc ggccaccgag acgtgcatgg cgaccgcggc ctcgctggac gagttccgcg
     2521 cggtggcgaa aggcgtcgag gcgcgcgggc ggcaggtcga acgctcgcct gcgtcgatct
     2581 tcaagggccc cgacgtcggg cgctacgagc tgctgggcga tctgcccgcc gcaggggcaa
     2641 cctgcccgct tcccggcgac gtgcgggcgc tgctcgggtg gagcgagcag gaggcggcgc
     2701 gcacggcggg cgcctacccg gcgcgccgct gatcacgaac gaaccacacc gccggcgcta
     2761 ccgctctgga tacagcgtca cccgcacgta ccgccccgga tccaggtagc gccgcgccgc
     2821 gtcctggacc atccgcggcg tgagctgccg gtactcgcgc ggcaggccga agtaggtcga
     2881 gaggtcctcg ccgttctcca accggtcgga gatctcgccc accagcgacg ggttgcgggc
     2941 aaggctcgtc tcgtgacgcc gcagcagcgc ctcgcgggcg tcgctcacct cgcggtccgt
     3001 cggacccttc tctcgcagcg cgccgatgtc ctggaacacc gacttcacca gctcgtcggc
     3061 cctcttgggg tcgcacccga agtcgatcgt cacgctgtat cgcggtgagg ggatcggctg
     3121 ggcggtcgcc cgcacctcga cgccgtagct cccctggagc gcctcccgga cggtgagtcg
     3181 caggcgattc tcgagcgcca gcgccagcgc gtcgagcgcc acccgctcgc tggccggggc
     3241 gttcatcggc ccggtgaaca cgatcgtcac ctggctcttc ggctcgatcc ccttgcgcac
     3301 ctccttctcg acgaccccgg ccgggggcgt caccccttcg tcgcgccagc tttccttccg
     3361 gccggccccg ggcagcgcgc cgaggtactg ctcgaccagc ggcttgatgg cctggggctc
     3421 gaagctgccg gcgaagacga aggtgaagtc gctcgcgtcg cggaagcggt ccttgtagaa
     3481 ggccagcgac tgctgcagat ccatctggcc caccatctcg gcggtcaggg gccgggcgcg
     3541 ggggtggtcc tgcgtcagcg ccgcctgcag cgtccggcgc agcgcccact cggggctcgc
     3601 ctcgcggctg gcgagcatcg cccgcatctg gtcctgcatc acgcggaaca cgttctcgtc
     3661 ggcgcgcggc tcggtgaacg agaggtagat cagctggaag agcgtctcga ggtccttcgg
     3721 cgacgccccg ccggccagcc cctcgctcgt cgggttgatg aagggcatga ccgaggcggc
     3781 cttgcccgac aggacgttgc gcagctggat ggcgtcgaac cgtcccacgc cgctcgctcc
     3841 cacgacctcg gcggcggtga tcgccgggac gtactgggcg tcgctcgcga gcgacgtccc
     3901 gccggggctc gtggcccgga agacgacctc gtcctgcctg aagtcggttg gcttcagcac
     3961 cacccggacg ccgttcgaca gcttccactc ggtgacgttg aaagcgagcg tgttggtgct
     4021 ctcgaccacg gcccctgact tcgggggggt ctccatgagc gcggtggcgc cgcccgcgtc
     4081 ggcgtaggcc tcgatctctc gctcgagcgc cgatttcatc accgcggcca gccgcgacgc
     4141 gtcgggcacc gtcagcccgg gcttctcggg cgcgttcacc aggacgacgc ggctgccgcc
     4201 cgcccagtcc ttcgccacct tgttgacctc ttcgagcgtg aagtggggga cgaagcgctg
     4261 caccaggccg aactcgtagg tgatgccggg gatcggctcg cgctcgacga agttgcggat
     4321 gtactcgtcg gccagatccg ccgactcctc cttctcgcgc tcgtcgaacg ccccctcgta
     4381 ggcgcgcagg atgttccgct tctcgcgctc cagctcggcg ggcgtgaacc cgaagcgggc
     4441 ggcccgggac gactcggtca ccagcgcctc gaggccccgc tcgatcccgt cttccttcac
     4501 gagggcgacc aggctcgccg tctccttggt ctgcacgaag atcccgcggt cggtcgccgc
     4561 gcgaaggaac ggcgggtcgg gcctgagcgc cagctcggcg agccggctgt tcagcatcgt
     4621 cgtgtagagc cgctcgacct gctgctggcg gtaggccccg acggtcgtct ggtcgcgaag
     4681 cgggagcttg ttgtagaggc cgacgctggt gatgggggcc tcgcggtcgg tggcgatggc
     4741 gaagagcgtc tcctcgtgat cgggcacctc gaagctgggg cgcgggcggg cggcgatggc
     4801 gccggggatc ggcgtgaagt ggcgcttgat cagcgcctcg atgtcgcgct tgtcgaagtc
     4861 gccgacggcg atgacggcca tcaggtcggg gcggtaccag tccttgtaga agcggcggag
     4921 cgcatcgggc ttgaaggtct gcaggctttc gcgggtgccg atcgggagcc ggtcggcgta
     4981 gcgcgatccc ttgagcagga tcgggacctg ctggtcctgc atccgcgcgc cggcaccgcg
     5041 gccctggcgc cactcctcca gcaccacgcc gcgctccttc tcgatcgact cggtgtcgag
     5101 cgtgagggcg tgggcgacgt cggcgaagaa gaggaacgcc ttgttcatcg cctcttcgtt
     5161 ggtcgtcggg acgtgcagct ggtagatggt ctcgtcgaac gaggtgttgg cgttgacgcc
     5221 gggcccgagc cgcatcccga tcgattccat gaaggtgatc aggtcctgct tggcgaagtg
     5281 cttgctgccg ttgaacgcca tgtgctcgag cacgtgggcc acgccgcgct ggtcctcgtc
     5341 ctcgaccacc gagccgacct tcaccaccag gcgcagctcg gcccgctgca ccgggcgctc
     5401 gttggtgcgg atgtagtacc gcaggccgtt cgggaggtac ccgatggtga tccgggggtc
     5461 gaccgggacg ggcgcgtcga gcgcgaccga ttcccacccg ggcttgtcct gcccggcgag
     5521 gctgcctgga gcgaagagga cgaggagaag tgcgagcagt ccgggcaggc ctgccgccgg
     5581 gcgccgggtc ctgattgtca tctctcgatc atcaggccac gctcaccaac cgtcaaggcg
     5641 gctgacacgg gctggcaccg ctcctgcaca cgccgccggc ccagtggacc tcgacagcgc
     5701 tccgacgcgt gatccagaat cgggggcatg cgctacctcc ccgcgctcgt cgtcggggcg
     5761 tcgcttggcg tgatcgcggg catcggcggc tacaccttcg tctacgcgaa gggggcgtcg
     5821 tacctgacca acgaccccgc cgcctgcgcc aactgccacg tgatgaacga gcagtacgac
     5881 gcgtggctca agtcgagcca ccacgcggtc gccgcctgca acgactgcca caccccccac
     5941 gccctcgtgc ccaagtacac gacgaaaggg ctgaacggct tctggcactc gtacgcgttc
     6001 accacgggtg acttccccga gccgatccag attacggggc gcaaccgccg cgtgactgag
     6061 ggagcgtgcc gcgagtgcca cgccgacatg gtgcacgcca tcgaaggcgt gccggccagc
     6121 gcggacgatt cggtctcgtg catccgctgc caccggagcg tcgggcacct gcactagggt
     6181 tcaggattcg ggagtcagga ttcaggagtc gggattcagg attcgggatt catatgaagc
     6241 tcaaacctcc agctctcgtc ctgttgaccg ccggcgtgac catgctcgtc gtcgccggcc
     6301 tgacggcgct cctgatgaac atccgcgagc acaaggagga agcccgcaac ccgttcttcc
     6361 gagtcgtcac cctgaccgac gacaccgagg accccgaggt ctggggcaag aacttcccgc
     6421 tccagcacga cctctaccgc cgcaccgtcg accaggtgcg caccaagcac ggcggaagcg
     6481 aggcgcagcc ccgcaccccg accgacgccg acccgcgctc gatcgtcgcg cagtcgcgcc
     6541 tcgaggaaga cccgcgcctg aagacgatgt ggaacggcta cgccttcgcg gtcgacttcc
     6601 gcgaggagcg cggccacgcc tacatgctcg acgaccagac ctttaccggg cggcagctcg
     6661 tgaccccgca gcccggcacc tgcctgaact gccacgcgtc gatgtacgtg acctacaaga
     6721 agctcggagg gggcgacatc gtcaagggct tcgaggcgat caaccggatg ccgtacgcgg
     6781 aggcgcgcaa ggccgtcaag cacccggtcg cgtgcatcga ctgccacgac cccgacacca
     6841 tgcagctcag ggtgacgcgc cccgcgttca tggaggggat tcgcgcgctg aaggcctcgc
     6901 agggggttgc caactacgac gtcaacacca tggcgacgcg ccaggagatg cgcgccttcg
     6961 tctgcggcca gtgccacgtg gagtactact tcaagggtgc cgagaagcgc ctggtctacc
     7021 cgtgggcgaa ggggatcagg gtggagaaca tcctcgacta ctacgaggag gtcggccaca
     7081 aggactggac ccacgccgac agcggggccg acgcgctgaa ggcgcagcac cccgagttcg
     7141 agatgtggaa ccagggggtc cacgcgcgat cgggcgtggc ctgcgccgac tgccacatgc
     7201 cgtacctgcg gcagggagca ctgaagatca gcgaccacca cgtgcgcagc ccgctgctga
     7261 acctctcgcg cgcgtgccag acgtgccacc gctggaccga ggaggagctg aaggcgcgcg
     7321 ccgagacgat ccaggaccgg acgttccaga cgcgcaacac cgcgatggac gccctggtcg
     7381 cgctgatcgg cgacatcaag gccgcgcgcg aggcgggcgt ccccgagccg cagctgcagg
     7441 aggcgcgccg ggcgcagcgg cgcgcccagt tcatggtcga cttcgtcgag gccgagaact
     7501 cgaccggctt tcacgccggc caggaggccg tgcggattct cgggcaggcc atcgacacgg
     7561 cccggcaggg gcagctcagc ctgaggccgc cggtcggcag gtaggcggcg aggacgccgg
     7621 cgcggaggcc ggcgttactg gatgccggcg ctactcgacg gcagagacgc tggcgcgggg
     7681 gcaggtgcgg catcgggttc cggctcccag cctgcagccg cccgcccctc ggcaaccacc
     7741 atgttcaccc gcagcagcac gcgcaggtag agcagcgcct gctgcgccgc cagcacgccc
     7801 gcgatgacga cccaggtgcg tcccccgggc gcgccgccga tccaggcgcc gagcgcgagc
     7861 gccgccgcaa tccccaggcc gtagaccacg cccggcatgg ccgccttcgc cgggtgccgc
     7921 agcacgaacc acgcgccgcg cagccaggtt cggacgaccc cgcgcgcgtc gtggcgcacc
     7981 atccaaacgc gtgcgtagtc gagcgcgagc acgaagtagg cggcaacgca ggccaccagg
     8041 gccatggtga gtgcggcctg cgcgacgtcg ccaggctcgc cggctcttgc caaaatcggc
     8101 tcgagcaccg ccccggcaag tgcggccgcg gtgcctccca ccgcgagcac ggccacggcc
     8161 gacagcgcga tcagcccgac cgacctgagg aagaagcggc cggccgcctc gaagaagcgc
     8221 gacagcaggc gcccttcggc ggctgcccgg ccggcggcaa gcgcgaagag gccgccgccg
     8281 agcagcgcgt tcgacaggag cgcgagccct atcgcggaaa tcagcgccgc cgggacgccg
     8341 cggagggagc cggcggagtc ctggagcagc tcgaacaagg tgccgaactg gatgccgtcg
     8401 agcagggggt cggtgcccgg cgcgacgcct gtttcccgcg acatccaggt gaagaacggg
     8461 aaggccacca cgagggcgag caccgtgtgc caggcccaca gcagtagtgc gatggcggcc
     8521 cgcctgggag agtcgggagg tgtcgtcatg gctacagccc caggaacatc agcagcgcct
     8581 gcacaccgaa caggaagcgc gacgtccacg atgccgccac cctcttgtcg ggcgacaccc
     8641 gccgcgcgtt gtccagccag ttcgcatcga gccagacctt gcgatccggg tccacggcgg
     8701 cccactcgag ccgctcggga cgggtgaacg tgaaacgctt ccaccggtcg cgcccgtccc
     8761 actgcaggcg ctcgaccggc ttcccctcga acttcaccgc cagcgtcacc gggaagacga
     8821 actcgccgtg ccgcgtcacc atcacgcggc tctcgtacat cttcgcttcg ccgcggggtt
     8881 cgggcgccgc gtcctgagcg aggctctttt cttccttttc cttctctgtc ccgccctcgg
     8941 ccttctgccc gccgcccggc tcgaacactc cccgcgctgc cttcgcctcg accgacgaca
     9001 cggctgtgac gccgtagtcg agcacgtgtc ccgtctcgaa ggcctggtgg aagaaccagc
     9061 tgaggtcgcg gcccgccacc tcgctggcgg tggcgaagaa gtcggcgctc gacgggtacc
     9121 ggaaccgcca ccgctcgtga tacgtccgca tcacgcgggc catcgtctcg tcgccgagca
     9181 gcccttgcag cgtgcgcagc gccgtcgccg gcttctggta ggcgtaggtc gagtagtcgc
     9241 cggcgtaccc ccacgccggc ttcatgatga cgtccttctg gtcgggcgtg gtggttaccc
     9301 gaagcacgtc gacgtccccg atccgcatcc ccggcagcga ggcgtacgag ctggcgtcgc
     9361 cgtactccgc gtccatggcc aggcccgtcg agtaggtgtt gatcccttcg tcgagccagg
     9421 cctcctcgaa ctcgttgttc gccaccatgc cgtaccagaa ctggtggccg aactcgtgca
     9481 cggtcaccat ctcggccgcc cggacgccgt cgagcggcca gaagttgccc agccagaacg
     9541 tgccgcccgt gatgaaagtc gggtactcca tcccgccggc gccgccggcc tcggcgggcg
     9601 gatcgacgac ggtcagcgtg cggtaggggt agcgcccgta ccagaggccg taccacttca
     9661 gccccgcctt cgccgcccgg atgtagcgtg cagcctgcgg ccggtgctcg ggctggagca
     9721 gcagggtgat cgcgacgtcg gacaaccgca cctcgtcgag cggtcgcccc agccgtgcgg
     9781 cggtcgcgcg gtactcggcg tcgctcacgt ccttgccggc cgagaacgtg tcgttcacca
     9841 ccaggtagcg gggcgacgcg gtccacgcga agtcgtgcac gtcggcctgc tcgaaaacgt
     9901 gggtcgtctg gccgctggcc tcctcgctgg ccacgcgcga gccggtggcg ccgacgacga
     9961 agcgtgacgg gagcgtgatt cgcacgcggt attcgccgaa gtcggcgtag aactcggagt
    10021 tcgcgtggta ctggtggcag ttccatccgc ccgacgcccg gccccggcgc ccggccggtt
    10081 cgtacacccc cagcttcggg aaccactgcc ccacgaggaa gaagtcgccc ttgtacccgc
    10141 tgcgggcgta gacctcgggc agctgtgcct tgaacgccag ctcgatggtc accgccccgt
    10201 tcggctcgac gggccggggc agcaccacgc gggccaccgt ccggtcgtcc gcgttgtcat
    10261 cgtccgggtg ctcgaagcgg atcgccggca tgaggtcggt gccgtcggcc agcttcagcg
    10321 aggtgatctc ggtccagccc cagttctcgg ttgcccggtc gttgcgcagg cggccgcccg
    10381 actcgcggaa gaaggtgctc ttcgtgttcc ggaaggcgtt caggtagagg tggaaccaca
    10441 aatcggggac ggggtcaccc gacgggttgc gccaggtgat cgtctcgcgt ccctcgagct
    10501 gcttcttctc ggggtcgagc cgcacggcga tgtcgtagcg gacgatgcgg tcggagcgct
    10561 cggggagcgg gccggccgag gattcgcccg ggggcgcgag aacgaggaga gcggcggaca
    10621 ggacaagggg gaagagcggg tgcggcagag cggccatgag agagtccctt ctggacagag
    10681 ggacgaacgg gcgacgcgag tgcggaaact cgaagggaga atgcagggtg acgaaggcag
    10741 gccgcccttc tacaccatgc attctccctt cgatacggct acccggtcgg gcgctactgc
    10801 gtgacgcgct cgatggtgac cgtcttcatg atgaccggct tggccggccg atcgcccggg
    10861 agcacccgca cggcgccgat cttcttcacc acgtccatgc cctcgaccac ctcgccgaac
    10921 accgagtgct tgttgtcgag gtgaggcgtc gggccgagcg tgatgaagaa ctggctcccg
    10981 ttggtgttgg ggccggcgtt ggccatcgag aggatccccg ccttgctgtg gcgcagcttc
    11041 gggtggaact cgtcggcgaa gcggtagcca ggaccgccgg tgccgttgcc gagcggatcg
    11101 ccgccctgga tcatgaagcc gttgatgatc cggtggaagg tgaggccgtt gtagaacggc
    11161 ttcgacttgc cgggcttgcc ggtggccggg tcgatcgtgc cctcggcgag gccggcgaag
    11221 ttggcgacgg tcttgggcgc ttccttgtcg aacagccgga ccgtgaactc gccttcggtc
    11281 gtgacgaagt gggcgtatgt gcctggcgtc aacttcttct ccttggcctg ggccggtgca
    11341 gcctgcgccg acgccacgcc gaccgacagg acgagggcgc cgcacaacgc ggcaaggaac
    11401 ttcgaggaca tcgggacgac tcctttcatc cggacaatgc tacttgggag ccccacggcg
    11461 tgcaacacac tgcgggggcg cgggcgaaaa cggggaaggg cggaacggcc ggccgatctg
    11521 atgcagaata ggggggcacc tatgtctcat gacgatctcc tgcaggccgc ggccgcgctc
    11581 gagcacggca cgcatcccga cccgtttgcc gtgctcggcc cacacccacg ggccggcggg
    11641 cggccggggg tggtggtcag ggccttctcg cccgacgcgg tcgctgccga gctgctgccc
    11701 gcgacgctgc ctccccagcc gatggcgcgc gtcggctcga gcggcctttt ccacgcggcc
    11761 atcgaggggt gggagggcga gccgctggcc ttcgactacc gcgtgaggtt cacgtggccc
    11821 gacggcagcg cgagcgaggt ggtcgacgcg taccgcttcg ggcgggtgct caccgacgaa
    11881 gatctctacc tgatcgccga gggcacgcag taccgggcgc aggacaagct gggggcgcgg
    11941 ccgctgcagt tcgggatcgt ctcgggcgtg cacttcgcgg tgtgggcgcc gaacgccgag
    12001 cgcgtgagcg tggtgggcga cttcaaccgc tgggacggcc gccgccatcc catgcggcgg
    12061 ctggcgccgc agggggtgtg ggagatcttc atccccgggc tcgccaacgg cgagaagtac
    12121 aagttcgaga tccgcgcccg cggcggcgcc gtgttccaga agaccgaccc gtacgcgcgc
    12181 cgcctggagg tgccgcccaa gtcggcgtcg atcgtctggc acccctcgcc ctacccgtgg
    12241 caggaccgcg agtggatgga gcggcgcgag cgcgagaacg ggtggctcga gcgcccggtg
    12301 tcgatctacg aggtgcacct cggctcgtgg gcgcgcgggg ccgacggcca gcgcttcctc
    12361 tcgtaccggg aactggccga gcgcctcgtc ccctacgtcc gcgacgccgg cttcacccac
    12421 atcgagctgc tgccggtgat ggagcacccg ttcgccgggt catggggcta ccaggtcacc
    12481 ggcttcttcg cccccacctc gcggttcggc gtgcccgaag acttcaaggc gttcgtcgac
    12541 gcctgtcacc tcgcgggcct cggcgtgctg ctcgactggg tgccggggca cttccccaag
    12601 gacgcgcacg ccctggcgcg cttcgacggg acggccctct acgagcacga ggatccgcgc
    12661 ctcggcgagc accaggactg gggaacgctg atcttcaact acggccgcca cgaggtgcgc
    12721 agtttcctgc tgagcaacgc cctgttctgg ctcgaggagt tccacgtgga cggcctgcgc
    12781 gtggacgccg tcgcgtcgat gctgtacctg gactactcgc ggcgcgaggg acagtggatc
    12841 ccgaaccgtt acggcgggcg cgagaacctc gaggcgatcg acttcctgcg cgagctgaac
    12901 tcgctcaccc acggcaagca cccgggcacg attaccgcgg ccgaggagtc gacggcgtgg
    12961 ccgggcgtca gccggccggt gcacctgggc gggctcgggt tcacctacaa gtggaacatg
    13021 gggtggatgc acgacatgct gtcgtacatg agccacgacc cgatccaccg gcggtgggcg
    13081 cacaaccaga tcaccttctc gatgctctac gcctaccacg agaacttcat cctgcccttc
    13141 tcgcacgacg aggtggtgca cgggaagggg gcgctcatcg ggaagatgcc gggcgacagc
    13201 tggcagcagg cggcgaacct gcgcgcgctc tacgggtaca tgttcggcca cccggggaag
    13261 aagctgctgt tcatggggga cgagttcggg cagcgggccg agtggaacca cgaccgcggc
    13321 ctcgagtggg agtacctcga cgtcttcccg cacggggggg tgctggcgct cgtgcgcgac
    13381 ctgaacgcct tgtaccgctc gcagccggct ctcttcgagc aggactacga ccccgcgggc
    13441 tttcagtgga tcgactgcaa cgacaacgag aactcggtcg tcacgttcct gcggcgtgct
    13501 cgcgacccgc gcgacttcct ggtgttcgtg ctcaacctca cgcccgtccc gcggcagggt
    13561 tacgtcgtcg gcgtgcccga gccgggcagc taccgcgagc tgctgaacac cgactctgaa
    13621 ctctacggcg gctcgaacct tggcaacggc gggttcgtgg ccagcgagcc cgtggccgcg
    13681 cacggctttt ccgaccggct tcgcctcgtg ctgccgccgc tcgcctgcct ggtgctgaag
    13741 atcgagcggt agcgcagtag cacaggcgtc ccgcctgtgg gctccagtag cacaggcgtc
    13801 ccgcctgtgg cctgtggcct gtggcggcca ggaggtctcc cttgacagca gcgggccgac
    13861 cgggcagtgt ggcctggaag ggccgcgcgg cggcccgttg cgagggggcc gcatcatggg
    13921 cgcagccgcc gacgcgagcc tgtccgggcc gctcgaggag acgatcaccc tcgggcaggc
    13981 gaagaggctc atccagtgca tggcgcacga gcacagcttc ctgctgctcg cgccgccggg
    14041 cgtagggaag tcggagacgg tctaccaggc tgccgaggag gcaggcctgc cctgccgctc
    14101 gctgctcggc acgcagatcg ctcccgaaga cgtgagcggc gttccgcgca tcgtcggcga
    14161 gcgatccgtc ttctgcccgc cgcgggtgct gctgcccgag aagcccgagc cgttctgcct
    14221 gttcctcgac gagctgccgg cgtgcgcccc cgacgtgcag aaggcgttct actcgctgct
    14281 gctcgagcgg cggatcggcg agttcgcgct gccgcccggg acgtgggtcg tggcggccgg
    14341 caatcgtgtg caggaccggg ccctcgtccg ctcgctcagc tccgccctgg tcaaccgcgt
    14401 gacgatcctg aacgtgcggg tggacgtggg cgagtggatg gcctgggcac ggggcgccgg
    14461 cgtccgcgag gaggttcgca cgttcatctc ctacatgccg gaggcgctga tgcgcccggt
    14521 gccgcaggag ccggtgccct tctcgacgcc ccgcagctgg acgctcttcg cccgggcgct
    14581 cgacctggcc gaggcggcgg gcgagctgac ccgcgcggcg cggcgcgcgc tcgccttcgg
    14641 gcggttgtcc gccgaggacg cggccgtgtt ctgcgcgctg gccgaggagt cgatcggcgc
    14701 gatgcagccg atcgcgtact acttcgccca ccccgaggcg ctgccgagcg gcgacacggc
    14761 gcggtggttc gtcctgaact gcatcaggca gaacgtgaag gacgggacgc tgcacgacct
    14821 cgcgccgcag gaggtgaacc ggttcctgcg cagcctgccg cacgagcacc agctcacgct
    14881 gctgaccgac ctggtggatc gctggggcgc cctcggggcc gacgaggcga tgttcgacct
    14941 gctgaaggag gtggcgcacc tgtgagcgcc ccctccagcg accgtgccct cgccgaccgg
    15001 atcgaacgcg ccctccggat ggtgaccgtc ccgttcccgc acctcgcggg gctggtcgcc
    15061 tcggcgcggg tgacgatcga cgagcgggtg ccgaccatcg gggtgttcgc ctccggccgc
    15121 ctgtcggcga acccggcctt cgtgcgcggc ctgaaggaga acgagctcgt cttcgttctc
    15181 gcccacgaga tgctgcacct cgccctccgc acgcacgacc gggcgagggg ggccgaccgg
    15241 ctcgagttca actacgcgca cgactacatc atcaacgaca tcctgcggac cgagctgggg
    15301 gcgcccgtgc cggccggagg cctcgacatg ccgggcgcgc gcgaccgctc ggccgaggaa
    15361 atcgtcctcg agatgcggcg gaaccagcag gggcagtcga agacgcgggt ctggcagggc
    15421 gaggcgacga cggcggggcg ggtgttcggg gccaggggac gaggcgccgg caccctggag
    15481 gcgggcgacg tgctcgacct gtcggccgag gccgacctct acccggaaga cgctgccgag
    15541 agcggtgagc gtgccgagca ggtgaaggag atggcggcca aggcgctcgg cctcgcgcgg
    15601 gcgatggggc tggcgcgcgg ggtgcggggc accgacgcgg gggcggcgag ccagacggtg
    15661 ctggcgctgc gggggatcta ccggaccccc tggaagctgg cgctgcagcg atggatcgag
    15721 tcggtggcgc ccggcgaccg taccttcagc cggccgtcgc gccgcgagat ctcgcagcct
    15781 gacgtcgtgc tgccgggacg ccgccgcgaa gggtggatcc tgaacatcgt gctcgatacg
    15841 agcgggtcga tgaccgacga gatcccgcgg gccctcggca cgctgggcga gttctgcgat
    15901 gccgcggggg tggaccaggt gcgtttggtg cagtgcgacg ccgaggtcac cagcgacgag
    15961 ctgctcgccc cgtacgagct ggccgagcgg cggatcgagg gttacggcgg gagcgacctg
    16021 tcgccggcgc tgcagcacct cgcgggcgac cagcacgtgc gggcggtcgt ggtgattacc
    16081 gacggggaaa tcgcgtaccc ggccgaggcg atgccgtacg agctgctgtg gctcgtgccg
    16141 cccggggtga gggccttccg tccaccctac gggatggtgg tcgtcatgca gcaggcgtag
    16201 caggtcttcg cgagcgacac gatgaaagcc atccaggaac tcgtggccta tttcgagcgg
    16261 cgggggcggc tgacgccgat ccaggtgctg aagctcctcg accaggggct gctggcgggc
    16321 gacgcgccgg cgaacatgct cgacctctgc gatcagatcg gcgccacgta ctacttcagg
    16381 ctgacgggcc aggcgacggg gatggtgtgg ggcaccgacg tctacaccgg cgactcctcg
    16441 cttgcggcgg ccgcggtgca cgcgggcgtc gtgaaggatg gggaagccgg ggtcgtcaag
    16501 gtgactgtcg cgccgccgct ctcgcggtac cagggttcat cccgctacgg cgtgacgagc
    16561 cacgacttcg gccactacgg caccgcgtac cgcctgtcgc gagtctaggg cctggcccca
    16621 aggtcaccgc gaggccagca gcgcctcgat ttcctcgacc ttcttcggca cgtccctggt
    16681 cagcacctcg cagccgtcct tggtgatcag caccgtgtcc tcgatgcgga tcccgaggtt
    16741 cttgtccggg aagtagaggc cgggctcgat cgcgaagacc attccctcgc ggagcaccgg
    16801 gccgagcggc acgtcgtgcg tcgagaggcc gacgccgtgc ccgaagccgc cgcgaagctc
    16861 gggcccctgc tcgaggccct tccgcttcat cacctcggcc acgtggcgcc gcacatcgtc
    16921 caccgtcgcg cccgggcggt acgcctcgat gcaggcctcg agcaccgcca ggacccgttc
    16981 gtacaccgcg cgctgctcgg gggtgaactt tcccgaggcc ggccacgtcc gcgtgatgtc
    17041 catcgcgagg tggtccatct ggccgccgaa gtccatcagc acgaggtcgc cctcctcgat
    17101 ccgccggccg ttgtcggcgt agtggaggat gcaggtgttc ggccccgacc ccacgatcgg
    17161 cgcgtacgcg gcgcccaacc cgccgctgcg cagcacctcg tgcatcgccg ccgcctcggc
    17221 ctcgtactcg taggcgcccg gccgcgccgc gagcatcgcc cgcttcaccg cctcggcgct
    17281 cacgcgcccg ttgcgcctca ggatctcgat ctcgcccggc gtcttgatcg cgcgcatggc
    17341 gtcgatgagc ggcgcgacgt cggccagcac ggcctggggg tagcgctcgc gcagccgcgc
    17401 cacccggtac tggtcgagcg agctctgatc gttgtagggc gtccttgccc gcctgccggc
    17461 gaacagcgcc gtctcgctcc gtgcgccgtc gagcaggtcg cgcggcgaca gccgaaggtg
    17521 gagggtcggc cccgccgcgg cagcggtccg tgccaggaac tcctcgaaga acgacaccgg
    17581 gtggatcgcc gtgacgccca gcttctcggc ccccttcgga tcgccgagca ggttgcggcc
    17641 gtccatcagc tcctcgcgcg cggccttctg ctggaggaac aggtagctgg ccttcgcacg
    17701 cggcgacatc acgaggatcg ccccggcgtc gttcgatccg gtgaagtagt agaagtcgtt
    17761 gtcctgcacg aagcgtgagc cggccggcgc gtccacctcc gcgaaaaaga cgatcaagcc
    17821 atcctgcacc ttctccatca acgcctgccg cctggcctgg tgctcggcga cggaaatcga
    17881 cggccgttgc gcgtcgagcg gcagcacggc cgcaaccgag gcggccaaac caaggacgag
    17941 ggtggaaacg aagccggacg tgcgcatctc tcctccgggc ggggggcact ctctccagcg
    18001 gccggcagca gtggcacagg cctcccgcct gtgtggctgt gg
//
LOCUS       DAOPQX010000030        17867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3775_length_17867_cov_4.852964, whole genome shotgun sequence.
ACCESSION   DAOPQX010000030 DAOPQX010000000
VERSION     DAOPQX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17867
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3775_length_17867_cov_4.852964"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PKK95_02970"
     CDS             complement(<1..981)
                     /locus_tag="PKK95_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC17199.1"
                     /translation="MPDAVVLKDLTKTFGETVAVRGLDLVVPEGALYGVIGPNGAGKT
                     TTIRMILAILFPDSGELQVLGRPSALEAKDRIGYLPEERGLYKKMRVAEFLLYMARLK
                     GMDGASLPKQVDAWLDRVGLLEAARKRCEELSKGMQQKIQFIAAVIHRPDLLILDEPF
                     SGLDPVNQRLLRDLVLEEHRRGATILFSTHIMVQAEQLCDHVVMIHRGEKVLDQPLST
                     IGSSFDPRSILFEPLDPASDVSVLQHLPGVRGVAREGGAWDVALAEGAEAGAVLRAIV
                     SAITPARIEVRRPTLEDVFVGIVAGTRAGAGSAALRQSLRTARPAAPRPRS"
     gene            1113..3569
                     /locus_tag="PKK95_02975"
     CDS             1113..3569
                     /locus_tag="PKK95_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17200.1"
                     /translation="MLPRNLSRTVRALLRSPLFTSVAVLTLALGIGAGTAMFSVVYGV
                     LLKPLPFPDANRLVAVWHSAPGINLEMLPQGPATYLTYREEGRTFEEIGLWQNGMVTV
                     TGLSEPERVPALMVTDGTLPILQVAPHLGRRFTRQDDSPAGPATVMLTYGYWERRFNA
                     DAAAIGRPILVDGQPHEVVGVLPRSFQFLDQEADLLLPLRLNRSEEFVGHFNYRGLAR
                     LKPGVTVEQANADIARMIPMVLDRFPLPPGFTRKMIDEARIGPNVRPLAADVIGDVGD
                     VLWVLFGTVGVVLLIACANVANLFLIRAEGRHQELAVRSALGARPSLIARGLLGESVT
                     LSLLGGVLGACFAYGGIRVLLAVAPDGLPRLREIAIDPVVLLFALGLSLAAGLVFGLL
                     PLLRFSTPRVLAGLKDGGRSASDGRQRNRARSALVVGEIAMALVLLVAAGLMIRTFQE
                     LRGVHPGFTRPGEVLTFRVSVPESLEADPAKIARLHEEVARKLGALPGVAAVGQCSAL
                     TMDGGSNDPVIVEEFPLAAGRFPPIRRMKWAAPGYFETMGNPIVAGRDFTWNDNYGLA
                     LVAVVNEAFVREYWKNPADALGKRIRSGPQEWRQIVGVVGDERDDGVARPAPPMVYWP
                     MLSKSLWEEGTYAARTMSYAVRSSRAGSPTLLAEIQRAVWSVNRNVPLANARTLEEIR
                     SESMAQTSFALVMLGIAAAVALSLGMVGIYGVIAYMAAQRTREIGIRMALGAQRENVS
                     GLFVRHGLVLTGAGVLVGVVAAAALTRLMSALLFGVGAWDPVTYAAVSIVLAAIALLA
                     SYVPARRASRLDPIAGLRGR"
     gene            3691..6147
                     /locus_tag="PKK95_02980"
     CDS             3691..6147
                     /locus_tag="PKK95_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17201.1"
                     /translation="MPQNVRSTIRALVRAPLFTLVAVVTLALGIGASTAIFSVVYGVL
                     LKPLPYDEADRLVAVWHTAPGINMPLLNQGPATYVTYREEGRAFEDIALWDGAAVSVT
                     GGRDPERIDALLVSDAFLTVLRVRPHLGRAFTAEDDSPATPERVMLSYSYWERAFGAK
                     ADAVGQQVIVDGKPREIIGVLPRGFRFLDRDADMLLPFRFNRAEMFVGNFSYQAIARL
                     KPGVGIEQANADVARMIPMVLDRFPTPPGFTRRMFEDIRLGPRVRPLAEDVIGDVGPV
                     LWVLLATVGIVLLIACANVANLFLIRAEGRQQELAVRSALGARRRAVAGQLVLESLLL
                     ALTGGALGLLLAHGGLVLLRLLAPDGLPRQNEIGLDPIVLAFTLALSLGAGVVFGLVP
                     LLHFSTPRVLAGLKDGGRAASDSRARNRARNALVVSEIALALVLLVASGLMLRTFQEL
                     RQVDPGFVRPAEVLTFRLSVPESVIPEAEASVRTYEEIARRIRQIPGVRSVGVSSSIT
                     MDGLDSNDAVFVEEFPPAPGRLPPVRRFKWIAPGYLETMGNELVAGRRFTWNDVYGMA
                     RVTMVSEAFAREYWKSPSDALGKRIKQGPDQPWREIVGVVGDERDDGMARPPTPIVYW
                     PMLLQWWQHEKWTPRGMAVAVRSATSGSPALLEQVQKAVWAVNPALPLANVSTLSAIQ
                     AESIAQTSFALVMLAIAAAVALALGVVGIYGVIAYMATQRTREIGIRMALGAEPGTVS
                     RLFVRHGLVLTGFGVTLGIAAAASLTRLMAALLFGVSAWDPLTYVVVSAGLGGIALLA
                     SYLPAWRASRLNPIVALRAN"
     gene            complement(6175..8388)
                     /locus_tag="PKK95_02985"
     CDS             complement(6175..8388)
                     /locus_tag="PKK95_02985"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOC17202.1"
                     /translation="MNTRLTLSVVATLLALGAAPAPQAAPEPFFPVAVWYSGGKARAP
                     MIAPLDAGSEQAWRNDLRTIKGLGFNTVRTWVEWSHGEPREGEYHLENLDLMLRLAEE
                     AGLKVLVQVYVDSAPDWVGRKYPDGHFTTQDGTQIKSQAAPGYCVDHPGVREALHGFF
                     TEVARHASASPAFYAYDLWSEPAVMNWALPAYVPNAQFCYCPHTQARFRKWLQEKHGT
                     LDALNRAWYRTYSDWQQVEPPRFGTILTYADFMDWRLFIGDKIAGDLEGRADAVRRVD
                     RSNPVTSHAPNPSPVFRTLADAMDASDDYLMKHAVDFFGTSFYPKLTSPDRDFPLERR
                     ALVMDMVRAVTGDRGFYVGELQAGYGVHGVIAGNPITGADLDLYTWGLVSRGARAISY
                     YAYYPMSTGYEAGGYGLINLDGTPTGRSRRAGATATLIAANAVALLSSRPQPAEVAVV
                     FNPLVPLLGGEQAYGDRRSVHRAVAGYHRMFFERNVAVDFPSARELDAASLARYKLVI
                     VPYPILLTAQMAKALEQYVAGGGRLFVEARPGWQDERGHAEPILPGFGWHEMIGVRET
                     GVLPVKEVEIRWGDRRFPGTGFAEHFERLDPAARVVATYPDGQPAAYEREHGKGRALL
                     LGTFAGERNQLEPLAMHPLGDALIDWAGITRPGLKSSAFVELRRMTGPSADFVFLFNH
                     GTAAAEVDLAVPLTAAPSAIRELVTGESLRPSGTTFGLKTTIPGETVRVYRLDRD"
     gene            8453..10027
                     /locus_tag="PKK95_02990"
     CDS             8453..10027
                     /locus_tag="PKK95_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC17203.1"
                     /translation="MTQQNTRPQTQPGAWASLIRAMRSWRTASVALLSFSSGLPLGLV
                     WYSIPDWMRDIGVDIRLVGLFTLAQAPWAFKVVWSPLMDRYVPPFWGRRRGWMAVTQL
                     ALAVFGLLLAGVGHRPEAIWVVGAIALAIALASASQDIAIDAYAVEVLRKEEQGAAVG
                     ARTALYRAAMLVSGGGAISLASRWGWPAVNVLLALVYLPILFLSWKSPEPEERQVAPR
                     TLREAVWLPFLGFLARHRALEMLAFVLLYKLGDQLTQALTRPFLIDMGYNADQRGIAL
                     ATIGMIATIAGAFIGGWITTLVGLGHSLWVFGFLQIFSNVGYYLVAIVGGVNLPLMFG
                     ATGFELFTGGLGTGAFSVLLLRMTQKRFSATQYALFSSLFALPRLLAGPIAGFSVDAM
                     GWPIFYLSTLLLGIPGLVMLARFVPLGVREPEFKVEEVARREPIAPGALVGRGVAGGL
                     LSGLFGLAVVALLAAIKTMRAAEGAGFDFQAALRQVMKPAGIADWVQLVGIAVFAVMG
                     GLFVAATVAARRGVRS"
     gene            complement(10017..10262)
                     /locus_tag="PKK95_02995"
     CDS             complement(10017..10262)
                     /locus_tag="PKK95_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17204.1"
                     /translation="MQHAGERRCGSCRFRAECHVCPIGRRWNTRAGESEAVPEYLCAF
                     NRLLQRHAGRFRRAAGLEVAGSARLLRRLLSQALVTI"
     gene            10628..12625
                     /locus_tag="PKK95_03000"
     CDS             10628..12625
                     /locus_tag="PKK95_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOC17205.1"
                     /translation="MTTSRVPSLAVMVSFVVLLVLGTGVSPHGPSAAQAPDRPPAAAP
                     QGLPNETPASLELPTGDFDHFRREVMIPMRDGVRLHTVVLVPKGAKRAPILLTRTPYS
                     ADVLTGHAASAHLGPVLQGYDNPADVIVEEGYIRVVQDVRGKYGSEGDYVMNRPLRGP
                     LNPTGVDHATDTWDTIDWLVKNVPESNGRVGILGISYDGFLTLMALFDPHPALKAAVP
                     MNPMVDGWVGADWFHNGAFRQQMMPYFFNQEATRDSTAQWWMTHYDDYDMFLEAGSAG
                     ELGRRRRLEQVGFWRKVTEHPSYDAFWQQQAVDKLLAARPLSVPVMLVHGLWDQEDIY
                     GALAVYRALEPKDSRNDRVYLAIGPWYHGQQIEEASRLGALHFNSDTARQFRHQVLKP
                     FLARFLKDGAPAMEVPPVTAFETGANRWLSLSAWPAGCASGCAIEPRRLYLGKGLTAG
                     FEEPKAEGAPFEEYVSDPAKPVPYRQRPIPPVGYGAASTWSSWLVDDQREASGRPDVL
                     AFVSPVLDAPVKIAGSPVANLVASTSGTDSDWVVKLIDVYPNEVAGQPELGGYQLMVA
                     GDIFRGRYRESLETPRAVPSDTPLPYRFALPAANHVFRRGHRIMVQVQSSWFPLYDRN
                     PQTFVPNIFFARPEDYRKAVQRVYHAPGQASFVELPIVVQR"
     gene            12756..14063
                     /locus_tag="PKK95_03005"
     CDS             12756..14063
                     /locus_tag="PKK95_03005"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOC17206.1"
                     /translation="MTDTKITLPESEIPTHFYNIAADLPTPLAPPLHPATREVVGPDA
                     LAPLFPSGLIAQEMASDRWIEIPDQVREIYRLYRPSPVFRARAFEEALDTPARIYYKS
                     EHVSPVGSHKLNTAIMQAWMNKQEGVKRLATETGAGQWGSALACASKLVGLECMVYMV
                     RVSYDQKPYRRVMMKTYGADVVASPSTLTEAGRSALAANPDSPGSLGLAISEAVEDAA
                     GRDDTKYALGSVLNHVLLHQTIIGQEARKQMELAGDYPDILIGCHGGGSNFAGLVFPF
                     LADKVSGARKNLRAIAVEPRSCPSLTAGKREYDFGDAVGLTPLLMMHTLGHTFMPAPV
                     HAGGLRYHGSAPLVSHVLDAGLIEAEAYDQAEVFASAVAFARSEGTIPAPESAHAIHA
                     TVRHALAAREAGTSPVILFNLSGHGIFDLAAYDSFLSGRINGK"
     gene            14176..15525
                     /locus_tag="PKK95_03010"
     CDS             14176..15525
                     /locus_tag="PKK95_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC17207.1"
                     /translation="MRLISCFTVAACIALAPAAPSAQKPARVAAAAIHERVAAAVGAI
                     RLVDTHEHLSAEAVRLKGEMSLFSLLHYVSSDMWADGLDRAASEAALGRSSLPLEKQW
                     EMIAPYWTNVRTTAYGRALLRAVRDLYGVNDISESTYLDISKRIREANRPGWQRQILE
                     KAGIDVSITDIGITGAALDPSHLRAVLRLDYFLVVPQGVMVAEREQGVTIATLADFEA
                     ALDRAVAAAREKKFVGIKSGVAYERSLEFGEVDRAEAERLFQELKQQKGKPGRADWTR
                     GKALQDYMFGRLAEACAKYDLPLQVHTGFFYDTWRNLAQANPTLLAPFVIRHRNTRFV
                     LMHGGYPYGTELLAMAKNLPNVTLDMCWTYIVSPSFAARFLNEAIETVPADKVLGFGG
                     DYQVAEGSYAHAMLCREVVSKVLAGKVADGYWSEAEALAYARGLLRENAIRVFKLNQ"
     gene            complement(15583..16899)
                     /locus_tag="PKK95_03015"
     CDS             complement(15583..16899)
                     /locus_tag="PKK95_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC17208.1"
                     /translation="MVGIDAGTIRRHLEVLTGEVGVRVAGSTAERRAAAYIAAELERR
                     GAAVWVEDFPVRERVVASQELEVLSGGSWRRFPCSLLSNAPGTDGRVVEAPIAVIDAA
                     TGYQREDLSYLRGKAVLHLGTHIESADHYRRLMEAAPAFLLFVDVRYPADVATADGMF
                     PAYVHHFGAVPTVSVAYQHAWSWMAEGAGAARLRVQGGMRESTSQNVIGELRGSDPSA
                     GVIYVGAHHDTQADSVGADDNATGVAAILALGGALADVPRRRTLRLISFGAEEQLSVG
                     SAMYVRRHREELVSGARFMFNFDTLGSHLGWTILTCNGPAGIAPVFVEALKARNQYLR
                     VTTELIPYADHFPFVAAGVPAAWMGRENCAAGRFFHHRPDDDMTRVSCELVAELSAGV
                     GVALQRLATAETLPFAPRTDAAGRLAVATMWEELFGGWEGFGAGGV"
     gene            complement(16919..>17867)
                     /locus_tag="PKK95_03020"
     CDS             complement(16919..>17867)
                     /locus_tag="PKK95_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17209.1"
                     /translation="YVAFLEGYARALVSVESRVDRARKWLREMLGTVRPEVRVEPGPG
                     GAPTLAVAFPSARTHQDVSRLAQEVFALPGRIADARDRTLVVALDEFQAVASFGAEGG
                     RTARMAQVEHALRAAVQHQRRVGYVFSGSEPSLMEAMLGRSRPFYKAGPVMRLEKIDP
                     AAFAAFVGARFRATRIRPEPGLGAAIVELAGNLPYDVQRLAHELWDDARAEGAKAVGV
                     EDLHRTLRRLLGEHEALFETAWQRLTLAQRAALRAAVLEDGRELLSADTRARYRLGGA
                     STVQASLNALVRDDVLAREGARYATVDSLFREWVARHTH"
BASE COUNT         2488 a         6342 c         6328 g         2709 t
ORIGIN      
        1 cgaccgtggc ctcggcgccg ctggccgcgc cgtccgcagc gactggcgca gcgcggccga
       61 cccggcccct gcccgtgtcc cggccacgat gccgacgaag acgtcctcga gggtcggccg
      121 gcgcacctcg atccgcgccg gggtgatggc cgagacgatc gccctgagca cggcgccggc
      181 ctcggccccc tcggccagcg cgacgtccca cgcgccgccc tcgcgcgcca cgccgcgcac
      241 cccgggcagg tgctgcagca ccgacacgtc ggaggccggg tcgagcggct cgaacaggat
      301 gcttcgggga tcgaacgacg acccgatcgt gctcaagggc tggtcgagca ccttctcgcc
      361 gcgatggatc atcacgacgt ggtcgcacag ctgctcggcc tggaccatga tgtgcgtgga
      421 gaagaggatg gtcgcgcccc ggcggtgctc ctcgagcacg agatcgcgca gcagccgctg
      481 gttcacgggg tcgaggccgc tgaacggctc gtcgaggatg agcaggtcgg gacggtggat
      541 caccgccgcg atgaactgga tcttctgctg catccccttc gacagttcct cgcaccgctt
      601 gcgcgccgcc tcgaggagcc cgacccgatc gagccaggcg tccacctgct tgggcaaact
      661 cgccccgtcc attcccttca ggcgcgccat gtacaagagg aactcggcca cccgcatctt
      721 cttgtagagc ccgcgctcct ccggcaggta gccaatgcgg tccttggcct ccagggcgga
      781 cgggcgcccc agcacctgca gctccccgct gtccgggaag aggatcgcca ggatcatccg
      841 gatggtcgtc gtcttgccgg cgccgttcgg cccgatgacc ccgtacaaag ccccctccgg
      901 gacaacgagg tcgaggccgc ggacagcgac ggtttcgccg aaggtcttcg tcaggtcctt
      961 gaggaccacg gcatcaggca tcgaaggctc cgtgtcgagg tgcaaccggc ggcggaaccg
     1021 agatgataca cccgcggcct ggcgcgctcg gaggggcggt gattcggccc gccgcgaacc
     1081 ggccctggcg accctgcgta taagcaggtg tgatgctgcc caggaacctg tctcgaaccg
     1141 ttcgcgccct tcttcgcagt ccgctgttca cctccgtcgc ggtgctcacc ctcgcgctcg
     1201 gcatcggggc cggcacggcg atgttcagcg tggtctacgg ggtcttgctg aaaccgctgc
     1261 cgttccccga tgcgaaccgg ctggtcgccg tgtggcactc ggcgcccggg atcaacctcg
     1321 agatgctgcc gcaggggccc gccacctacc tcacctaccg ggaggagggc cggaccttcg
     1381 aggaaatcgg tctgtggcag aacggcatgg tgacggtcac cgggttgagt gagcccgagc
     1441 gggtgccggc cctcatggtg accgacggca cgctcccgat cctgcaggtc gcgccgcatc
     1501 tcggccgccg cttcaccagg caggacgact cgccggccgg cccggccacg gtcatgctga
     1561 cctacggcta ctgggagcgc cgcttcaacg ccgacgcggc cgcaatcggc aggccgatcc
     1621 tcgtcgatgg ccagccgcac gaagtcgtcg gcgtgctgcc gcgcagcttc cagttcctcg
     1681 accaggaagc cgatctcctc ctgcccctgc ggctgaaccg ctcggaggag ttcgtcgggc
     1741 acttcaacta ccgcggcctc gcgcgcctga agcccggggt aaccgtggag caggcgaacg
     1801 ccgacatcgc acggatgatc ccgatggtcc tcgatcgctt cccccttccg cctggcttca
     1861 cgcggaagat gatcgacgag gcgcgcatcg gaccgaacgt ccggccgctc gccgccgacg
     1921 tgatcggtga cgtgggcgac gtgctctggg tgctgttcgg cacggtcggg gtcgtcctgc
     1981 tgatcgcgtg cgcgaacgtg gcgaacctgt tcctgatccg cgccgagggg cggcaccagg
     2041 agctggccgt gcggagcgcg ctcggcgccc gcccgtcgct gattgcccgc gggctgctgg
     2101 gcgagagcgt gacgctgtcg ctgctggggg gcgtgctcgg cgcctgcttc gcctacggcg
     2161 ggatccgcgt cctcctggcc gtggcgcccg acgggctgcc gcgcctccgc gagatcgcga
     2221 tcgacccggt ggtgctgctg ttcgcgctcg gcctgtcgct ggccgccggc ctggtcttcg
     2281 gcctcctgcc gctcctccgc ttctcgacgc cgcgcgtcct tgccgggctg aaagacggcg
     2341 ggcgatcggc gagcgacggc cggcagcgga accgcgcccg cagcgcgctg gtggtgggcg
     2401 agatcgcgat ggcgctcgtg ctgctggtgg cggcggggct gatgattcgc acgttccagg
     2461 agctgcgcgg cgtgcacccg ggattcacgc gccccgggga ggtcctcacg ttccgcgtct
     2521 cggtgcccga atcgctggag gccgacccgg cgaagatcgc gcggctgcac gaggaggtcg
     2581 cacggaagct cggcgcgctc ccgggcgtcg ccgccgtcgg ccagtgctcg gcgctcacca
     2641 tggacggcgg cagcaacgac ccggtcatcg tcgaggagtt ccccctggcc gcgggccggt
     2701 tccccccgat tcggcgcatg aagtgggccg cgccgggcta cttcgagacg atgggcaacc
     2761 cgatcgtcgc cggccgcgac ttcacgtgga acgacaacta tggcctcgcg ctcgtggcgg
     2821 tcgtcaacga ggcgttcgtg cgcgagtact ggaagaaccc cgccgacgcg ctcggcaagc
     2881 ggatccgctc ggggccgcag gagtggcgac agatcgtagg ggtcgtcggc gacgagcgcg
     2941 acgacggggt ggcgcggccg gcgccgccga tggtctattg gccgatgctc tccaagagcc
     3001 tctgggagga aggcacctac gccgcccgga cgatgtcgta cgccgtgcga tcgtcgaggg
     3061 ccgggtcgcc gacgctgctg gccgagatcc agcgcgcggt ctggtcggtc aaccggaacg
     3121 ttccgctcgc caacgcccgg acgctcgagg agatccggtc cgagtcgatg gcccagacct
     3181 cgttcgcgct ggtcatgctg ggcatcgcgg ccgcggtggc gctgtcgctg ggcatggtcg
     3241 ggatctacgg ggtgatcgcc tacatggccg cgcagcgcac ccgcgagatc ggcatccgca
     3301 tggcgctcgg cgcacagcgc gagaacgtga gcggcctgtt cgtgcggcac ggcctcgtgc
     3361 tgaccggcgc gggagtgctg gttggagtgg tcgccgcggc ggcgctcacg cgcctgatgt
     3421 cggcgctgct cttcggcgtc ggggcctggg acccggtgac ctacgccgcc gtctcgatcg
     3481 tgctggcggc gatcgccctg ctggcgagct acgtgcccgc ccgccgggcg tcgcgcctcg
     3541 acccgatcgc cggcctccgg gggcggtgag ggtggccccg gcgtcgcgtc gctgacgtcg
     3601 gggcaacccc tgtgtgcccg gctgaacccg cccgcccggc ctcccgtcta acctcccagg
     3661 cggtcggatc gagtcccctg ggagaacctc atgccgcaga acgttcgcag caccatccgg
     3721 gccctcgtgc gtgccccgct gttcacgctg gtcgccgtgg tcacgctcgc gctgggcatc
     3781 ggggcgagca cggccatctt cagcgtcgtc tacggcgtgc tgctcaagcc gctcccgtac
     3841 gacgaggccg accggctggt cgccgtgtgg cacaccgcgc ccgggatcaa catgcccctg
     3901 ctgaaccagg gaccggccac ctacgtgacc taccgggagg aggggcgtgc cttcgaggac
     3961 atcgccctgt gggacggggc agcggtctcg gtaacagggg gccgggatcc agagcgaatc
     4021 gacgcgctcc tcgtcagcga cgccttcctc acggttctgc gcgtgcgccc ccacctgggc
     4081 cgggcgttca cggccgaaga cgactcgccc gccacgcccg aacgggtgat gctgagctac
     4141 tcctactggg agcgggcgtt cggagcgaag gccgatgcgg tggggcagca ggtgatcgtg
     4201 gacggaaagc cgcgcgagat catcggggtc ttgccgcgcg gctttcgctt cctcgaccgc
     4261 gacgccgaca tgctgctgcc cttccgcttc aaccgggccg agatgttcgt cgggaacttc
     4321 agctaccagg cgatcgcgcg gctgaagccg ggggtcggca tcgagcaggc gaacgcggac
     4381 gtggcccgga tgatcccgat ggtgctcgac cgcttcccca ccccgccggg gttcacgcgc
     4441 agaatgttcg aggacatccg gctgggcccg agggtccgtc cgctcgccga ggacgtgatc
     4501 ggcgacgtgg gcccggtgct gtgggtcctg ctcgccacgg tgggcatcgt gctgctgatc
     4561 gcctgcgcga acgtcgcgaa cctcttcctg atccgtgcgg aggggcggca gcaggagctg
     4621 gccgtgcgat ccgcgctcgg ggcgcgcagg cgtgctgtcg cgggtcagct ggtgctcgaa
     4681 agtctcctgc tcgcgctgac ggggggcgcc ctcggcctcc tgctcgccca cgggggcctc
     4741 gtgctgctgc ggctgctcgc gccggacggc ctgccgaggc agaacgagat cgggttggat
     4801 ccgatcgtgc tcgccttcac gctggccctg tcgcttggag ctggcgtggt gttcggcctc
     4861 gtcccactac tccacttctc gacgccgcga gtgctcgccg gcctcaagga cggcgggcgc
     4921 gcggcgagcg acagccgcgc gaggaaccgc gcacgcaacg ccctggtggt gtcggagatc
     4981 gccctggcgc tggtgctgct ggtcgcgtcg ggcctgatgc tccgcacctt ccaggagctg
     5041 cgccaggtcg atcccggctt cgttcgcccc gcggaggttc tgaccttcag gctctcggtt
     5101 cccgagagcg tgatccccga agccgaggcc tcggtccgca cctacgagga gatcgcccgg
     5161 cggattcgcc agatacccgg cgtacgctcg gtgggcgtgt cctcgtcgat cacgatggac
     5221 ggcctcgaca gcaacgacgc cgtcttcgtg gaggagttcc cgccggcacc cgggcgcctg
     5281 ccgccggtcc ggcgcttcaa gtggatcgcg ccgggctacc tcgagacgat gggcaacgag
     5341 ctggtggccg ggcgccgctt cacctggaac gacgtctacg ggatggcgcg cgtgaccatg
     5401 gtgagcgagg cgttcgcgcg cgagtactgg aagtcgccct ccgacgcgct cggcaagcgg
     5461 atcaagcagg ggcccgacca gccctggcgc gagatcgtcg gcgtggtggg cgacgagcgc
     5521 gacgacggca tggccaggcc gcccacgccg atcgtgtact ggccgatgct gctccagtgg
     5581 tggcagcacg agaagtggac gccgcgcggc atggccgtcg cggtccgctc ggctacttcg
     5641 ggctcgccgg ccttgctcga gcaggtgcag aaggcggtgt gggcggtgaa cccggcgctg
     5701 ccgctcgcca acgtgagcac gctctccgcc atccaggccg agtcgatcgc gcagacctcc
     5761 tttgcgctcg tgatgctggc gattgcggca gccgtggccc tcgcgctcgg ggtcgtcggg
     5821 atctacggcg tcatcgccta catggccacg cagcggaccc gggagatcgg gatccggatg
     5881 gcgctcgggg ccgagcccgg cacggtcagc cgtctcttcg tcaggcacgg tctcgtgctc
     5941 acggggttcg gcgtgacgct ggggatcgcg gcggctgcgt cgctgacgcg gctgatggcg
     6001 gcgctgctct tcggggtgag cgcgtgggat cccctcacct acgtcgtggt gtcggcgggg
     6061 ctgggcggga tcgcgctgct ggcgagctac ctgccggcct ggcgcgcctc ccggctgaat
     6121 ccgatcgtgg cgctgcgagc gaactgaccg ccgccgggcg cccagcggcc aaggttagtc
     6181 ccggtccagc cggtacaccc gcacggtctc acccgggatc gtcgtcttga ggccgaaggt
     6241 ggtgcccgag gggcgcagcg attctcccgt caccagctcg cggatggcag agggcgcggc
     6301 cgtcaagggc accgcaagat ccacctcggc ggcggcggtg ccgtggttga acaggaagac
     6361 gaagtcggcg gacgggcccg tcatccgccg cagctccacg aacgccgacg acttcaggcc
     6421 cggcctggtg attccggccc agtcgatcag ggcatcgccg agcgggtgca tcgcgagggg
     6481 ctcgagctgg ttgcgctcgc cggcgaaggt tccgagcagc agcgcgcgtc ccttgccgtg
     6541 ctcacgctcg taagcggccg gctgcccgtc cgggtaggtc gccaccacac gtgcggcggg
     6601 atcgaggcgc tcgaagtgct cggcgaagcc ggttccgggg aagcggcgat cgccccagcg
     6661 tatctccacc tccttcaccg gcagcacccc ggtctcgcgc acgccgatca tctcgtgcca
     6721 cccgaagcct gggagaatcg gctcggcgtg gccccgctcg tcctgccagc cggggcgcgc
     6781 ctcgacgaag aggcgcccgc cgcccgcgac gtactgctcg agcgccttcg ccatctgcgc
     6841 cgtcaacagg atcgggtagg ggacgatcac cagcttgtag cgggcgagcg acgcagcgtc
     6901 cagctcgcgg gcgctcggga agtcaaccgc gacgttccgc tcgaagaaca tgcggtggta
     6961 gccggcgacg gcgcggtgaa cggatcggcg atcgccgtac gcctgctcgc cgccgagcag
     7021 cggcacgagc gggttgaaga ccaccgccac ctcggccggc tgcgggcggc tcgagagcag
     7081 cgccacggcg ttcgcggcaa tcagcgtcgc ggtcgcgccc gcccgccggc tgcggccggt
     7141 tggcgtgccg tcgaggttga tcaggccgta cccgcccgcc tcgtacccgg tgctcatcgg
     7201 gtagtaggcg tagtagctga tcgcgcgggc gccccgcgac accagccccc acgtgtagag
     7261 atcgaggtct gcgccggtga tcgggttccc cgcgatcacg ccgtggacgc cgtaccctgc
     7321 ctgcagctcg cccacgtaga agccgcggtc gcccgtcacc gcgcgcacca tgtccatgac
     7381 cagcgcacgc cgctcgagcg ggaagtcgcg atcgggggag gtcagcttcg ggtagaagct
     7441 ggtgccgaag aagtccaccg cgtgcttcat caggtagtcg tccgacgcgt ccatcgcgtc
     7501 ggccagcgtc cggaagaccg gggacgggtt gggcgcgtgg ctcgtcaccg ggttggaacg
     7561 atccacgcgc cgcaccgcgt cggcccgccc ctcgagatcg cccgcgatct tgtcgccgat
     7621 gaagaggcgc cagtccatga agtcggcgta ggtgaggatc gtgccgaagc ggggcggctc
     7681 cacctgctgc cagtcgctgt aggtgcggta ccaggcccgg ttgagcgcgt cgagcgtgcc
     7741 gtgcttctcc tgcagccact tccggaagcg tgcctgcgtg tgtgggcagt agcagaactg
     7801 cgcgttgggc acgtaggccg ggagggccca gttcatcacc gccggctcgc tccagaggtc
     7861 gtaggcgtag aaggccgggc tcgccgacgc gtggcgcgcc acctcggtga agaacccgtg
     7921 cagcgcctcg cgaacgcccg ggtggtccac gcaatacccc ggcgcggcct gcgacttgat
     7981 ctgcgtgccg tcctgggtgg tgaagtgccc gtccgggtac ttgcgcccca cccagtcggg
     8041 cgcggagtcc acgtagacct gcacgagaac cttcaggccg gcctcctcgg cgagcctcaa
     8101 catcaggtcg aggttctcca ggtggtactc gccctcgcgc ggttcgccgt ggctccactc
     8161 gacccacgtg cggacggtgt tgaagcccaa ccccttgatg gtccgcaggt cgttccgcca
     8221 cgcctgctcc gagccggcgt cgagcggggc gatcatcggc gcccgtgctt tccccccgct
     8281 gtaccagacg gccaccggga agaagggctc gggggccgcc tgaggcgctg gtgcggcgcc
     8341 gagggcaagg agggtggcaa cgacggagag cgtaagtcgg gtgttcatgg gttttcggtt
     8401 ctcagcgccg gcgtcggctg tcggctgcct gccgacagct tatactcccc gggtgacgca
     8461 gcagaacacc cggccgcaga cccagcctgg cgcctgggcc agcctcatcc gtgcgatgcg
     8521 ctcgtggcgc accgcctcgg tggcccttct ctccttctcc tccggcttgc cgctcggtct
     8581 cgtctggtac tcgatccccg actggatgcg cgacatcggc gtggacatcc ggctcgtcgg
     8641 cctgttcacg ctcgcccagg ccccgtgggc cttcaaggtc gtctggtcgc cgctgatgga
     8701 tcgctacgtg ccccccttct gggggcgccg gcgcggctgg atggccgtga cccagctggc
     8761 cctcgcggtg ttcggcctgc tgctggccgg cgtcggccac cggcccgagg cgatctgggt
     8821 ggtcggcgcg atcgccctcg ccatcgcgct cgcgtcggcc tcgcaggaca tcgccatcga
     8881 cgcgtacgcg gtcgaggtgc tgcgcaagga ggagcagggg gcggcggtcg gcgcgcgcac
     8941 cgcgctctac cgcgcggcca tgctcgtctc gggcggcggc gcgatctcgc tggccagccg
     9001 gtggggctgg cccgcggtca acgtcctgct cgccctggtc tacctcccga tcctcttcct
     9061 gagctggaag tcgcccgagc ccgaagagcg ccaggtggcc ccccgcacgc tgcgcgaagc
     9121 ggtctggctg ccgttcctgg gcttcctcgc ccggcatcgc gcgctcgaga tgctcgcctt
     9181 cgtgctgctc tacaagctcg gcgaccagct gacgcaggcg ctgacgcgcc cgttcctgat
     9241 cgacatgggc tacaacgccg accagcgcgg catcgcgctc gccacgatcg ggatgatcgc
     9301 caccattgcc ggcgccttca tcggcggctg gatcaccacg ctcgtcggcc tcggccactc
     9361 gctctgggtg ttcggcttcc tccagatctt ctcgaacgtc ggctactacc tggtcgcgat
     9421 cgtggggggc gtcaacctgc cgctcatgtt cggcgccacc ggcttcgagc tgttcaccgg
     9481 cggcctcggc accggcgcct tctcggtgct gctgctgcgg atgacccaga agcgcttctc
     9541 ggccacgcag tacgcgctct tctcgagcct cttcgcactt ccccgcctgc tggccgggcc
     9601 gatcgccggc ttctcggtgg acgcgatggg ctggccgatc ttctacctgt ccacgctcct
     9661 gctcggcatc cccggcctcg tcatgctggc gcgcttcgtc cccctcggcg tgcgcgagcc
     9721 ggagttcaag gtcgaggagg ttgcgaggcg cgagccgatc gccccggggg ccctcgtcgg
     9781 gcgcggcgtg gccggcggcc tgctgtcggg cctcttcggc cttgccgtcg tggcgctgct
     9841 cgccgcgatc aagacgatgc gcgcagccga gggagccggg ttcgacttcc aggcggcgtt
     9901 gcggcaggtg atgaagccgg caggcatcgc cgactgggtt cagctggtgg ggattgccgt
     9961 cttcgccgtg atggggggcc tgttcgtcgc ggccaccgtc gcggcgcgac gcggcgtcag
    10021 atcgtgacga gggcctggct caacagccgc cgcagcaggc gggccgagcc cgccacctcc
    10081 aggccggccg cccgtcgaaa gcgcccggcg tgtctctgca gcagccggtt gaaggcacac
    10141 aagtactcgg gtacggcctc ggactcgccg gcgcgggtgt tccaccttcg gccgatcggg
    10201 cagacgtggc actccgcgcg gaaccggcac gacccgcacc gtctctcgcc cgcgtgctgc
    10261 acccgcgtgc tgcaggcgct ggggtgcgaa gaggcctgac gcggaaacct gtccgtccac
    10321 gtccacgctc agcgagcggc ccgatgacgc gctgcacgcc cacttccggg ctccacgcgg
    10381 gcgctcgcgg gtggatgggt ggatggcggc gacgccggtg atcaccttga cggccaccgt
    10441 acgcccgcgc cgcgtgtcgc gagcgcggta aaccgctccc atcccgccgg caccgagcag
    10501 attcgggatt tcgtacgggc cgaggcgcat gccggccgcc agggccgggc gcggaaatca
    10561 gccctccggg aacggctggc cggcgagcgc cgcctggggt agtctctccc ttcgaaaggg
    10621 gaagcgcatg acgacgagcc gcgtgccgag cctggcggtg atggtttcat tcgtggtcct
    10681 cctggtcctc ggcaccggcg tgtcgccgca cggcccttcc gccgcgcagg cgccagaccg
    10741 gccgccggcc gccgccccgc aggggctgcc gaacgagacg cctgcgtcgc tcgagcttcc
    10801 aaccggtgac ttcgatcact tccggcgcga ggtgatgatc ccgatgcgcg acggggtcag
    10861 gctgcacacg gtggtcctgg tgccaaaggg agcgaagcgg gccccgatcc ttctcacgcg
    10921 gaccccctac agcgccgacg tgctcaccgg ccacgccgcg agcgcgcacc tcggccccgt
    10981 cctccagggt tacgacaacc cggccgacgt gatcgtcgag gagggctaca tccgggtcgt
    11041 gcaggacgtc cgcggcaagt acgggtcgga aggcgactac gtgatgaacc gcccgttgcg
    11101 cgggccgctg aaccccaccg gtgtggacca cgcgaccgac acgtgggaca cgatcgactg
    11161 gctcgtgaag aacgtgcccg agagcaacgg gcgtgtcggc atcctcggca tctcgtacga
    11221 cggcttcctc acgctgatgg cgctcttcga cccgcacccg gcgttgaagg cggccgtgcc
    11281 gatgaacccg atggtggacg gctgggttgg ggccgactgg ttccacaacg gcgccttccg
    11341 gcagcagatg atgccgtatt tcttcaacca ggaagcgacg cgcgactcga cggcgcagtg
    11401 gtggatgacg cactacgacg actacgacat gttcctggag gccgggtcgg ccggcgagct
    11461 gggccggcgc cggcgcctcg agcaggtggg cttctggcgg aaggtgaccg agcacccgtc
    11521 gtacgacgcg ttctggcagc agcaggcggt ggacaagctg ctggcggcac ggccgctctc
    11581 ggtgccggtg atgctcgtgc acgggctctg ggaccaggag gacatctacg gcgcgctcgc
    11641 ggtgtaccgc gccctcgagc cgaaggactc gcgcaacgac cgcgtgtacc tggcgatcgg
    11701 cccgtggtat cacgggcagc agatcgagga ggcgagccgc ctcggcgcgc tgcatttcaa
    11761 ctcggacacg gcccgccagt tcagacacca ggtgctgaag ccgttcctcg cgcgcttcct
    11821 gaaggacgga gcgccggcga tggaggtgcc gccggtcacg gcgttcgaga cgggcgcgaa
    11881 ccggtggctg tcgctgtcag cctggcccgc cggctgcgcg agcggctgcg ccatcgagcc
    11941 ccggcgcctg tacctcggca agggcctgac ggcgggcttc gaagagccga aggccgaggg
    12001 ggcaccgttc gaggaatacg tgtccgaccc ggcgaagccg gtgccctacc gccagcgccc
    12061 gatccctccc gtcggctacg gcgcggcttc gacctggtcg tcgtggctgg tggacgacca
    12121 gcgcgaggca tccgggcgtc ccgacgtgct cgcgttcgtc tcgcccgtgc tcgatgcgcc
    12181 ggtgaagatc gccggcagcc ccgtggcgaa cctggtcgcc tcgacgagcg gaacggactc
    12241 ggactgggtg gtgaagctga tcgacgtcta ccccaacgag gtggccgggc agcccgagct
    12301 ggggggatac cagctgatgg tggcgggcga catcttccgg gggcgttacc gcgagagcct
    12361 ggaaacgcca agggcggtgc cgtccgacac gcccctgccc taccggttcg cgctgccggc
    12421 ggcgaaccac gtgttcaggc gcggccaccg catcatggtg caggtgcagt cgagctggtt
    12481 cccgctgtac gaccggaacc cgcagacctt cgtgccgaac atcttcttcg cccggcccga
    12541 ggactaccgc aaggcggtgc agcgcgtcta ccacgccccc ggccaggcca gcttcgtcga
    12601 gctgcccatc gtcgtgcagc gttgaagccg ccgtctgctg tctgccgtct gccgtccgct
    12661 tcgggcaggc ggggacgcct gcccctgccc gactggtgtc ccctgccgtg tgtgctatcc
    12721 ttcgcctttt acgccgtgaa acaaggggtt ctgccgtgac cgacacgaag attacgctgc
    12781 ctgagtctga gatcccgacc catttctaca acatcgccgc cgacctgccg acgccgctgg
    12841 cgccgccgct ccaccccgcc acgcgcgagg tggtgggccc cgacgcgctg gcgccgctct
    12901 tcccgtcggg cctgatcgcg caggagatgg cgagcgaccg ctggatcgag attcccgacc
    12961 aggtgcgcga aatctaccgg ctctacaggc cgagccccgt gttccgcgcg cgggcgttcg
    13021 aggaggcgct cgatacgccg gcgcgcatct actacaagtc cgagcacgtg agcccggtgg
    13081 gcagccacaa gctgaacacc gcgatcatgc aggcctggat gaacaagcag gaaggggtga
    13141 agcgcctggc gaccgagacc ggcgccgggc agtggggcag cgcgctggcc tgcgcctcga
    13201 agctggtcgg cctcgagtgc atggtctaca tggtgcgggt cagctacgac cagaagccgt
    13261 accgccgcgt gatgatgaag acgtacggcg ccgacgtggt ggcgagcccc tccacgctca
    13321 cggaagccgg ccgctcggcg ctcgcggcga accccgactc gccgggcagc ctcggcctgg
    13381 cgatctccga ggcggtcgag gacgccgccg gccgcgacga cacgaagtac gcgctcggca
    13441 gcgtgctgaa ccacgtgctg ctgcaccaga cgatcatcgg ccaggaggcc cgcaagcaga
    13501 tggagctggc gggcgactac cccgacatcc tcatcggctg ccacggcggc ggcagcaatt
    13561 tcgcgggcct cgtgttcccg ttcctggccg acaaggtctc gggggcgcgg aagaacctgc
    13621 gggcgatcgc ggtggagccc cgctcgtgcc cgagcctcac ggccggcaag cgtgagtacg
    13681 acttcggcga tgcggtcggc ctcacgcccc tgctgatgat gcacacgctc gggcacacgt
    13741 tcatgccggc tccggtgcac gccggcggcc tgcgctacca cggatcggca ccgctcgtca
    13801 gccatgtttt ggacgccggc ctgatcgagg ccgaggcgta cgaccaggcc gaggtcttcg
    13861 cgagcgcggt cgccttcgcg cggtcggagg gcaccattcc ggcacccgag tcggcgcacg
    13921 cgatccacgc cacggtccgg cacgccctgg cggcgcgcga ggcgggaacc tcgcccgtca
    13981 tcctgttcaa cctgtcgggc catgggatct tcgacctggc ggcgtacgac tcgttcctga
    14041 gcggacggat caacggcaag tagcttgccc gccggagccg cgcctgcccc gccgcagccg
    14101 cgagcgcagc gagcgtttgg cggaggcggg agcgtagcga gcgctggcga aggcggggcg
    14161 aaggccggag gcaccatgcg actcatcagc tgcttcaccg tcgcggcgtg catcgccctc
    14221 gctccggccg ctccctcggc ccagaagcca gcccgggtag cggcggccgc gatccacgag
    14281 cgggtggcgg ccgcggtcgg cgccatccgc ctggtggaca cccacgaaca cctgtcggcg
    14341 gaagcggtgc ggctgaaggg cgagatgtcg ctcttcagcc tgctccatta cgtctcgtcc
    14401 gacatgtggg ccgacggcct cgaccgcgcc gcgtccgagg cggcgctcgg ccgctcgtcg
    14461 ctcccgctcg agaagcagtg ggagatgatc gcgccctact ggaccaacgt gcgcacgacc
    14521 gcctacggcc gggcgctgct gcgggcggtg cgcgatctct acggcgtgaa cgacatctcc
    14581 gaatccacgt acctggatat ctcgaagaga atccgcgagg caaaccgccc gggttggcag
    14641 aggcagatcc tcgagaaggc gggcatcgac gtctcgatca ccgacatcgg catcaccggc
    14701 gcggccctcg acccctcgca cctgcgggcc gtgcttcgcc tggactactt cctcgtcgtc
    14761 ccccagggcg tgatggtggc cgagcgcgag cagggcgtga ccattgcgac gctcgccgac
    14821 ttcgaagccg cgctcgaccg ggcggtcgcc gcggcgcgcg agaagaagtt cgtgggcatc
    14881 aagtcgggcg tcgcctacga gcggagcctc gagttcggcg aggtcgaccg ggctgaggcc
    14941 gagcggctgt tccaggaact gaagcagcag aagggcaagc cgggacgcgc tgactggacg
    15001 cgcgggaagg cgctgcagga ctacatgttc ggccggctgg ccgaggcgtg cgcgaagtac
    15061 gacttgccgc tgcaggtcca cacgggcttc ttctacgaca cctggcgcaa cctcgcgcag
    15121 gccaacccga cccttttggc gccgttcgtc atccggcaca ggaacacgcg cttcgtcctg
    15181 atgcacggcg gctaccccta cgggaccgag ctgctcgcga tggcgaagaa cctgcccaac
    15241 gtcaccctcg acatgtgctg gacgtacatc gtctcgccct cgttcgccgc gcggtttctg
    15301 aacgaggcga tcgagacggt gccggccgac aaggtcctcg gcttcggcgg cgattaccag
    15361 gttgccgagg ggtcctacgc ccacgcgatg ctctgtcgcg aggtggtgtc gaaggtgctg
    15421 gccggcaagg tggcggacgg gtactggagc gaggcggagg ccctggccta cgcccgcggg
    15481 ctgctgcgcg agaacgccat ccgggtcttc aagctgaacc agtagtacag gcgtcccgcc
    15541 tgtgggtggc cacgaccgca ccgcagggct gaagccctgc tgctacacac cgcccgcgcc
    15601 gaatccctcc cagccgccga acagctcctc ccacatcgtg gcgacggcca gccggccggc
    15661 cgcgtcggtg cgcggcgcga acgggagcgt ctcggccgtg gcgaggcgct gcagcgccac
    15721 gcccaccccg gcggacagct cggccaccag ttcgcaggat acccgcgtca tgtcgtcgtc
    15781 ggggcgatgg tggaagaagc ggccggccgc gcagttctcg cgccccatcc aggcagccgg
    15841 cacgccggcc gcgacgaacg gaaagtggtc ggcgtaggga atcagctccg tggtgaccct
    15901 caagtactga ttgcgagcct tcagcgcctc cacgaagacc ggcgcgatgc ctgccggccc
    15961 gttgcacgtg aggatcgtcc agccaaggtg cgagccgagg gtgtcgaagt tgaacatgaa
    16021 gcgggccccg ctcaccagct cttcgcggtg gcggcggacg tacatcgcgc tgcccaccga
    16081 cagctgctcc tccgccccga agctgatcag ccggagcgtc cttcgccgcg gcacgtcagc
    16141 cagcgcgccg ccgagcgcga ggatggccgc gacgccggtc gcgttgtcgt cggcgccgac
    16201 cgagtcggcc tgggtgtcgt ggtgggcgcc gacgtagatc acgcccgccg agggatcgct
    16261 gccgcgcagc tcgccgatca cgttctggga cgtgctctcg cgcatgccgc cctgcacgcg
    16321 aagccgggcg gccccggccc cttcggccat ccacgaccag gcatgctggt aggcgacgct
    16381 caccgtcggc acggcgccga agtggtgcac gtaggcgggg aacatcccgt cggccgtggc
    16441 gacgtcggcc gggtagcgca cgtccacgaa gaggaggaag gccggtgcgg cctccatgag
    16501 ccggcggtag tggtcggccg actcgatgtg cgtcccgagg tggaggaccg ccttgccgcg
    16561 aaggtacgac aggtcctcgc gctggtatcc cgtcgcggcg tcgatcacgg cgatcggcgc
    16621 ctcgacaacc cggccgtccg tcccgggcgc attgctcagc agggagcagg ggaacctgcg
    16681 ccacgatcca ccggagagca cttccagctc ctgcgaggcc acgacccgtt cgcggacagg
    16741 gaaatcttcc acccagaccg ccgccccgcg ccgttcgagt tcggcggcaa tgtaggcggc
    16801 cgccctgcgc tcggccgtcg aaccggcgac ccggacgcca acctcacccg tcagcacctc
    16861 gaggtgccgc cgaatcgtcc ccgcatcaat tccaaccatc ggctcgactc ctccaggcct
    16921 agtgtgtgtg gcgtgcgacc cattccctga acagcgagtc caccgtcgcg tagcgcgccc
    16981 cctcgcgcgc cagcacgtcg tcgcggacga gggcgttgag ggaggcctgc acggtggagg
    17041 cgccgcccag gcggtagcgg gcccgcgtgt cggcggagag cagctcgcgg ccgtcctcga
    17101 ggacggccgc ccggagcgcc gctcgctggg cgagcgtgag gcgttgccag gcggtctcga
    17161 agagcgcctc gtgctcgccg agcaggcgcc ggagcgtccg gtgcaggtcc tcgacgccga
    17221 cggccttcgc cccctccgcc cgcgcgtcgt cccacagctc gtgcgcgagt cgctgcacgt
    17281 cgtacgggag gttccccgcc agttcgacga ttgcggctcc gaggccgggc tcgggccgga
    17341 tccgggtggc gcggaagcgc gctccgacga aggcggcgaa ggcggccggg tcgatcttct
    17401 cgagacgcat caccgggccg gccttgtaga aaggacggct gcgcccgagc atcgcctcca
    17461 tcaggctcgg ctccgacccc gagaagacgt aaccgacccg ccgctggtgc tgaacggcgg
    17521 cgcgcagcgc gtgctccacc tgcgccatgc gggccgtccg gccaccctcg gcgccgaacg
    17581 aggcgaccgc ctggaactcg tccagcgcga cgacgagggt gcggtcgcgc gcgtcggcga
    17641 tccggcccgg cagggcgaac acctcctggg ccagcctcga gacgtcctgg tgcgtgcggg
    17701 ccgacgggaa ggccaccgcg agggttggcg cgccgcccgg gcctggctcg acgcgcacct
    17761 ccggccggac ggtccccagc atctcgcgca gccacttccg ggcccggtcc acgcggctct
    17821 ccaccgagac gagcgcgcgg gcgtagccct cgaggaaggc cacgtac
//
LOCUS       DAOPQX010000031        17765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3800_length_17765_cov_4.927273, whole genome shotgun sequence.
ACCESSION   DAOPQX010000031 DAOPQX010000000
VERSION     DAOPQX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17765
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3800_length_17765_cov_4.927273"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..371
                     /locus_tag="PKK95_03025"
     CDS             45..371
                     /locus_tag="PKK95_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17210.1"
                     /translation="MSAGRALAGVLVVFAATAVLHPLLGGSARGQLVEVVAVCAIAYA
                     SFSVTFGRIRWPFLALVTAVFLVLRAADLYWLPEPLLSGPLSWVVVPALVAALLLVGR
                     RYFFTG"
     gene            complement(607..2106)
                     /locus_tag="PKK95_03030"
     CDS             complement(607..2106)
                     /locus_tag="PKK95_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOC17211.1"
                     /translation="MRLPVVLHLVGGILRLYAAAFLGPVAVAALYGEWWDLAGFTLAG
                     VVTLAASVAAWRAAPDVPSDFRRAEGMAVVSATWLAAAAFSALPYLWVGLGPTDALFE
                     AMSGITASGGTVLTDFDRYGRALFFWRGLTQWIGGMGVIALFVAVLPRLAIAGRELFF
                     AEAPGPTDEKIAPQIRRTAAGLWKLYTALSAAMTAALLATGMGLYDSLCHTFAAVGAG
                     GFSPHPQSLIGYENPAAEWVITAFMFLAGANFALQYRALRGERRALVADEEFRTYTSL
                     VLVSAALVAAFLWRADVPAATAIRHAFFQVVSILTTTGFAAADYNQWPEQAKMILLAL
                     MFVGGCAGSAGGGPKVVRHLMVARYTLKELARTLHPRAILPVKLGGRVVPDEVMRAVV
                     VFMLLYVLVFAVITGLVTLLGADLVTAATATIASLGNVGPGMGRVGPMDSFAHLHPLS
                     KVVLVVAMWLGRLELITVLALLQPGLWRGATWRQRPAGPTPRVPPSVTP"
     gene            complement(2110..3459)
                     /gene="trkA"
                     /locus_tag="PKK95_03035"
     CDS             complement(2110..3459)
                     /gene="trkA"
                     /locus_tag="PKK95_03035"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOC17212.1"
                     /translation="MHLIIIGGGEVGHALARALSDAHDVVVIDHDPTVGERFSSLDVE
                     FLHGSGTSTAMLRRAGIERCDLLVGCTGLDEVNVVACALANRVGSPKTICLVSKEDFL
                     QPDGGGDLLREQFGIDRILWPEAQLADDIVRVIKAPGAIDAEAFLGDRVRLLEYRLEA
                     GSSLARGPLATLKLPPGALAVAVKRGEEFLIPRGKTQLAAGDKVFFMGTHEAMHEVQS
                     RVVAARESRARQVVTIIGGGDVGFRVAQQLDRSPDIELRVIESNPKRGEMLAASLSRA
                     LVLQGDGTDLALLEAEEIGRSDVLVSVIDNDERNLFASLLGRQLGVRKIITRVSKPGS
                     LRLFERVGIDVPLSARGAAVASIVHGVAGGPSNLLAVLEEGQAEILEIKVPAGYTPTP
                     LLKLQAPPDSIVGAILRGDQVIVPHGSDVIQGEDTLLVFTTAASTPQVRDFFQPWSG"
     gene            complement(3479..4477)
                     /locus_tag="PKK95_03040"
     CDS             complement(3479..4477)
                     /locus_tag="PKK95_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOC17213.1"
                     /translation="MPTLLSPLRFTTLHLRNRIVMPPMCSGLATPEGFVTPPIVEYHR
                     RRAAAGCGLIIVEHTFVHERGRHSQGQMGVHNDATIEGLGRLAAAVKAEGAAVCLQLA
                     HAGSRGSRRVLGAAPLAPSALRHPYEPEGDVPEEMKVPQIQEVIAAFGEAAGRAAAAG
                     FDAVQIHAAHGYLLSQFLSPLTNRRTDDYGGPLEQRARLHREVVSSVRRRVGAHFPVF
                     VRLGADDETPGGLGIEDGASVAACLADNSVNLIDVSGGLQGSRGAGKGSAYFLPLAQA
                     IRSRVGVPVLVTGGISDAAMADEIVREEWADLVGIGRAMLNDPDWARQAIDQLKAS"
     gene            complement(4607..6733)
                     /locus_tag="PKK95_03045"
     CDS             complement(4607..6733)
                     /locus_tag="PKK95_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOC17214.1"
                     /translation="MSLRSRLTLAFFAISVIPLTVVTLYSYSTSRAALRHAAEQQARN
                     LAAELGQRMDWVTRDLERRIDKLWTAPAEGLWPADDHVAEGDIATLTPRPAKRLPAEA
                     IAGELVSTLGEVAPMLERLEIVPESIATGGREVPGAPEPAAPPAPPAPAAAAAPPAGK
                     VDAMAERAAKGRDSLIAVDGAGRLTVEVPKMIETAMAKYRAAGASAAGQAEFAAWARD
                     LQMQIQRGVGDLQALSVEAARRGERSAAGAGGAPARPPKPAAVKVKTVTVFKGNDLSC
                     SVEENGQQVARIKARLNAKRMLAPVLANTRRHQGEIPFAVDADGQVHTPAGGDRQIAA
                     LRPASLARVDGTATRTIDDWVVATHKDRTGVTVGIARPIADDLRELQRAAALNFGMGF
                     GLIALVFAGSLPLASGMTRHLRSLMAGVQRIAQGDLSTRIPVRSRDEFGELAGAFNQM
                     AQDLAAHQALLVEQERLHRELELCRQIQTGILPCEPLRLGVTEVKGVSIPAREVGGDF
                     FNYFVLPSGDIALLVGDVSGKGVGAALLMANVQATLRARLPLEQDLAALADALDADLE
                     AHTPPEVFLTLFVGILDPRRGVLRYVNAGHNTQFVLRPGGLDRLTTTGMPLGLFAGHG
                     YQERSVTLAPGDLLFFYTDGMVETENEAGDVWGIERLESLLVAAGPREVDSLLGRIEQ
                     DVRAFRGRAEPLDDATMMALRFGEPA"
     gene            6933..7394
                     /locus_tag="PKK95_03050"
     CDS             6933..7394
                     /locus_tag="PKK95_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOC17215.1"
                     /translation="MLDFCPRWIAERLGHSADPVYFATWRVSGWQPGLEPAEREVVAQ
                     VLQLHRDARCTMLAFVVMDDHVHVLLRCRRGSVDSVLEAIKTYSAHRLRDVYGRSGAI
                     WQHDWFGRKVQGEEDLRRQTEYIVSNPWKRWPFTEGYPWVWPYSSLTHIQR"
     gene            complement(7364..7759)
                     /locus_tag="PKK95_03055"
     CDS             complement(7364..7759)
                     /locus_tag="PKK95_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17216.1"
                     /translation="MGDPSFITWRLAPGQRRLDSRERDLVASVLRRGDGFRYTLLAFV
                     VMDDHVHALARAGSWPTDRIVGSWRSSTEQQLQRIHGRRGEIWAAGHQAQAVRTPQRR
                     RDVELSIVGNPWKRWPFLHGYRWMWVREE"
     gene            7946..8506
                     /locus_tag="PKK95_03060"
     CDS             7946..8506
                     /locus_tag="PKK95_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOC17217.1"
                     /translation="MSPWEIGGIAVGLAADASAVALAAGSSALARGFRFRFRLAFHFG
                     LFQFMMPLAGWLVGARVARYLGAVDHWVAFGILAYVGGRMIADGLSAKPRTAPRDPSR
                     GLTLVMLSVATSLDAFAVGLSLAMIDAGIWWASVTIGAVTGLLSAIALRAGSVLGAAA
                     GRRMEVAGGLVLVAVGLRILGQHLMA"
     gene            8554..9018
                     /locus_tag="PKK95_03065"
     CDS             8554..9018
                     /locus_tag="PKK95_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOC17218.1"
                     /translation="MTTLVDRVAALVEAFEGRPTWDEYFMAAALLMASRSACGRLHVG
                     CVLVSAGAYPHRVIAAGYNGFLPGCAHQSRLRDGHEQATVHAEQNAIADAARRGVSVG
                     GATAYVTHFPCINCAKILAAAGVRAVKYHHDYDHDPLVDALCGEAGIGLVQL"
     gene            9214..9726
                     /locus_tag="PKK95_03070"
     CDS             9214..9726
                     /locus_tag="PKK95_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOC17219.1"
                     /translation="MQLTRAADYGVRVMMHLVCQASGTRASLTDLAEAADVSPAFLSK
                     VLQRLVRAGLLASRRGKRGGFEMLEKGRQASLYDVLEALDGLPTLNVCLLDSGSCHRS
                     PWCAAHTVWTGAQQRLLQTLRETPIEGLAWDSHTRRLSMLPPSGAAQGQGPVRANGHG
                     EGNGWCPVES"
     gene            9781..11283
                     /locus_tag="PKK95_03075"
     CDS             9781..11283
                     /locus_tag="PKK95_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOC17220.1"
                     /translation="MSQRYLLGLDIGSSFVKASLLDVATGRAVAAASAPASEMAIAAP
                     QPGWAEQDPREWWRHGVAATREVLTRAGAPASAVAGIGISYQMHGLVLVDRRGQPLRP
                     SIIWCDSRAVPVGEAAFERIGRLTCLARVLNSPGNFTAAKLAWVRANEPDVFAAADKA
                     MLPGDYVAFRLTGRAATTASGLSEGVLWDFLDRRVSADVLGALDLPGRLLPELVPTFG
                     EQGVVSAEAAAETGLAAGTPVSYRAGDQPNNAFSLRVLHPGEVAATAGTSGVVYAVID
                     TAAWDNESRVNTFLHVTDHPGAPRYGVLLCVNGTGALNSWLKRLLGGEQVSYDEMNRL
                     AAEVPIGAGGVVVLPFGNGAERSLGNREPGASVHHLSLVRHGRPHVLRAAQEGIVFAL
                     HHGMGIMREMGLQATAVRAGRANMFLSPVFAQAFADLSGATVELFDTDGAQGAARAAG
                     LGAGVFATEEEAFNGLRPLDRLDPDAGRQAQYVDAYARWQAALKKELERG"
     gene            complement(11360..12322)
                     /locus_tag="PKK95_03080"
     CDS             complement(11360..12322)
                     /locus_tag="PKK95_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOC17221.1"
                     /translation="MSDWSQLLMTDHETTERVLEAVERTFEAPGGPAPSILRDALEYF
                     QGYVEACHNKKEEQHLFPLIERRGIPRAGGPLAVMLAEHDRGTSLLPRLVAAGEAYLG
                     GGHEVLPELRALLGEYAALLKDHYWKENDILYPMARRVMSEADAAAVVAGIEAVEAAL
                     GPDTRSRYYALAEHIIEAGELKDLSFGVERDVLAAILNTLPVELSFVDKDDRVRYFSH
                     EGGEKIFPRTRGAIGTAVQNCHPQKSVHLVNRILADFKAGSRKVAEFWIDMGGRKVHI
                     RYFPVRSPKGEYLGTLEVVQDIAPIQALQGQRRLLEEEETTAQG"
     gene            12593..15088
                     /locus_tag="PKK95_03085"
     CDS             12593..15088
                     /locus_tag="PKK95_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC17222.1"
                     /translation="MLVSLSPRPARPFLVSLALLLLAIPAAAQQAPALSVNAGGGLPS
                     PGVLPARPVPGLVLAEVHLRDLPSADTIWSVIETADPFVVVDRIANGGLYLGEHEWFG
                     SHGSSGTQSSFLMDGLDLTDPERTGTPLMQLDREYFQAVGVAYSFLAPELGGAGTTLV
                     LVPRAAGSRWAGQLRAGYLPHQLQAENARDGAPSIARFGTTGGAQVRVGGPVAERLGV
                     FLTGRGVASRRFERGDQTPLDSRLVSGFLHATASLSENHRLRVVAAADRAAHPFYARA
                     RFPLATVRATDNSRHLHATYERVTPRGTAFSAAAGYQRSETGGPSDGHGTVTGVIERI
                     LDGPVPELVVPPVGPRGRYGAVFQLNPGFGLDRLGRLSAGLSLNHAFSQPAIQARALV
                     GELVDGLPARVWDFESGSGEVRRHATELAAWVNGQVALGSRLALDLGARLDWTEARSE
                     GRDEMIRWRTVLPRGLLQWQITDGGGINAFLGYGLYRHRLPLGYLAYGDPGAPAGLVY
                     RWDDRNGDRWLQPGEVGRLVAAAGPGARSYFESRIDPDLEAPIARQLVLGFDLRMGER
                     WRWKTTGFERRDVGLVAPVNEGVTLADYRPIDIEDRGADFLNPIDDRLLTVYDRLPSS
                     FGRDRDVLTNPDGHDAHYIGVDVALERVFDGRWHMLFGAAAHRSDGLAGNRGFHVNEN
                     DVGVLGEAFQNPNALTYTRGRLFFERGYIIKWSGGVVTASGLHLGAVARYQDGQHFAR
                     MVIVPDLAQGPEAVQAYTRGHSRFTFTFTLDARIEQAFRVGGARLGLMVEAFNLLNTA
                     NEVEEDPVTTRLFRASTVVQPPRAIRFGLRYDF"
     gene            complement(15106..15897)
                     /locus_tag="PKK95_03090"
     CDS             complement(15106..15897)
                     /locus_tag="PKK95_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC17223.1"
                     /translation="MDEHEPRVSRLVVVMAALGGVVLLVGLLLWAVGGGDRPPAQPEA
                     ESPARPAPQPSAATVPEPVPGKPAEEPSERPRARRRAPEAPKPAPEPAPPTLGELRID
                     SDVPGAMVFLDRQYLGETPVTARGVAPGTHTLNLSADGYEGQSRTIEVEPGPSDVMVR
                     FKEIRLDEALDVVHKHGVGSCEGRLVATPRGLRYETTQKNDAFSLSFDQLESFEVDYL
                     KKNLRVKRRGGKTWNFTTHTESADPLFVFHREVQKVRERLAAGAK"
     gene            complement(15919..>17765)
                     /gene="guaA"
                     /locus_tag="PKK95_03095"
     CDS             complement(15919..>17765)
                     /gene="guaA"
                     /locus_tag="PKK95_03095"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOC17224.1"
                     /translation="RMAEEHEAVHDAIAVVDFGGQYAHLIATKVRRLHVLAEIRQPED
                     PLEAFRKYRGIILSGSPALSSFGEDSEYTKGIYDLDVPIVGFCFGHQEIAKHYGGQVV
                     HGGREWGPASLHLVRDDHPLFAGLGKIERVWMSHFDSVVSVGPGFEELGYTALGEGGE
                     GHRFAAIGSDTHRRYGVQFHPEVDDTEHGDEMIANFVLRICGCRPSWTMERYLEEQVE
                     RVRRQVGDRSVFLLASGGVDSTVAAKLFAMAVGADRLHLLHVDNGLMRKDESRKVVEM
                     LRALGLGRHVHFVDASDDFLRALAGVVAPERKRAIIGETFIEVFEREARRLGIEDHLL
                     GQGTIYPDTIETGGTKRADTIKTHHNRVPIVEEMIAAGKVVEPLAELYKVEVRELGEQ
                     LGLPRELLWRHPFPGPGLGVRLLCSDGIEDREGFGEIEPAAARVAGEYGLSTLALPIR
                     SVGVKADLRSYEHPVLLHGDPGWERVLEAAGMIFRHVPGVNRCVWNVGPRLPREARPV
                     PAGVTRERLDLLREADAIVMDGLRRSGSYDRIWQCPTVLVPLALDGQGRELIVVRPIH
                     SERAMTATPARLPETLLDEWQRSILALPGVAGLAIDVSTKPPGTIEWE"
BASE COUNT         2452 a         6365 c         6263 g         2685 t
ORIGIN      
        1 gcccagactg gtactggaac cagtggtgag aagggagggg cctcgtgagc gcgggtagag
       61 ccttggccgg cgtgctcgtg gtcttcgcgg cgacggcggt cctccacccg cttctcggcg
      121 gcagcgcccg ggggcagctc gtggaggtgg tggcggtctg cgcgatcgcc tacgcgtcgt
      181 tctcggtgac gttcggccgc atccgttggc cgttcctggc cctggtgacc gccgtgttcc
      241 tggtgctccg ggcggccgac ctctactggc ttcccgagcc gctgctgtcg gggccgctct
      301 cctgggtggt cgttccggcc ctcgtcgcgg cgctgttgct cgtggggcgc cgctacttct
      361 tcaccgggta gccgacggtt tgcgcgacga ccgtggccag cccctcggag gcccagaaga
      421 ggtacacgtt cgggcgatgg ggcagggtct ggccttgcga acacgtcgaa cgtgctcgca
      481 aaggcaggcc ctgctccatc gtcccgccgt ctccggcggc cgaaggccgg catgtgccgt
      541 cggcgcgccc ctttgtgact tttgtgacct ttgtgattga gatcgtcgtc cggcgtcgtc
      601 atctgctcac ggcgtgactg acggcgggac acgcggagtg ggaccggccg gccgctgccg
      661 ccacgtggca ccccgccaga ggccgggctg cagcagcgcg agcaccgtga tcagctccag
      721 gcgtcccagc cacatggcca cgaccaggac gaccttgctg agcgggtgca ggtgggcgaa
      781 cgagtccatg ggccccacgc gccccatgcc ggggccgacg ttgccgagcg aggcgatcgt
      841 ggcggtggct gcggtcacga ggtcggcgcc gagcagcgtc accagcccgg tgatcaccgc
      901 gaagacgagc acgtagagca gcatgaacac cacgacggcg cgcatcacct cgtcggggac
      961 gacgcggccc ccgagcttga cggggaggat ggcgcggggg tgcagcgtgc gcgcaagctc
     1021 cttcagggtg tagcgggcga ccatcaagtg gcgcacgacc ttgggcccgc cccccgccga
     1081 ccccgcgcag ccgccgacga acatgagggc gagcaggatc attttcgcct gctcgggcca
     1141 ctggttgtag tcggccgccg cgaaccccgt ggtcgtcagg atcgaaacaa cctggaagaa
     1201 cgcgtgccgg atggcggtgg cggccggcac gtcggcgcgc cagaggaaag ccgcgaccag
     1261 cgcagcggac accagcacga ggctcgtgta cgtgcggaac tcctcgtcgg ccaccagtgc
     1321 ccgccgttcg ccgcgcagcg cgcggtactg cagcgcgaag ttcgccccgg cgaggaacat
     1381 gaacgcggtg atcacccact cggccgccgg attctcgtag ccgatgagcg actgcgggtg
     1441 gggagagaac ccgccggcgc cgaccgcggc gaaggtgtgg cagagcgaat cgtacagccc
     1501 catccccgtg gcgaggagcg cggcggtcat cgccgccgac agagcggtgt agagcttcca
     1561 gagccccgcg gcggtccgcc ggatctgcgg cgcgatcttc tcgtcggtcg gtcccggagc
     1621 ctcggcgaag aacagctcgc ggccggcgat ggcgagccgc ggcagcaccg cgacgaacag
     1681 cgcgatgact cccatgccgc cgatccactg ggtcaggccg cgccagaaga acagggcccg
     1741 cccgtaccgg tcgaagtcgg tcagcacggt tccgccgctc gccgtgatgc ccgacatcgc
     1801 ctcgaacagc gcgtcggtcg gtccgaggcc gacccagagg tagggaagcg ccgagaacgc
     1861 cgcggccgcg agccacgtgg ccgagacgac ggccatcccc tcggcgcgcc ggaagtcgga
     1921 gggcacgtcg ggtgcggccc gccacgccgc caccgaggcc gcgagcgtca cgacgcccgc
     1981 cagcgtgaag cccgcgaggt cccaccactc tccgtaaagg gccgcgaccg ccaccgggcc
     2041 gaggaaggcg gcggcgtaga gccgcaggat tccgcccacc aggtgcagca cgacggggag
     2101 gcgcatcggt cagccgctcc agggctggaa gaagtcgcgc acctgcggtg tcgaggcggc
     2161 ggtagtgaac accagcagcg tgtcctcgcc ctggatcacg tcgcttccgt gcgggacgat
     2221 cacctggtcg ccgcgcagga tcgcgcccac gatcgagtcg ggcggcgcct gcagcttcaa
     2281 gagcggcgtc ggggtgtagc cggccggcac cttgatttcg aggatctccg cctggccctc
     2341 ctccagcacg gcgagcaggt tcgacggccc tcccgccacg ccgtgtacga tcgaggcgac
     2401 cgcggcgccg cgagccgaca gcggcacgtc gatgccgacg cgctcgaaca gccgcaggct
     2461 gccgggcttg ctcacgcgcg tgatgatctt gcggacgccg agctggcggc cgagcagcga
     2521 cgcgaacagg ttgcgctcgt cgttgtcgat caccgacacc agcacgtcgc tgcgcccgat
     2581 ctcctcggcc tcgagcagcg ccaggtccgt cccgtcgccc tgcagcacga gggcgcggct
     2641 gagggaggcc gccagcatct cgccgcgctt cgggttcgac tcgatcaccc gcagctcgat
     2701 gtcgggcgac cggtcgagct gctgggccac ccggaagccg acgtcgccac ccccgatgat
     2761 cgtcaccacc tgcctggccc gcgactcccg cgcggccacg acgcgcgact gcacctcgtg
     2821 catcgcctcg tgcgtgccca tgaagaacac cttgtcgccc gcggccagct gcgtctttcc
     2881 ccgaggaatc aggaactcct cgcccctctt gacggccacc gccagcgcgc ccggcggcag
     2941 cttgagcgtg gcgaggggcc cccgcgcgag cgacgacccg gcctccagcc ggtactccag
     3001 gagccggacg cggtcgccga ggaacgcctc cgcgtcgatc gccccggggg ccttgatgac
     3061 gcgaacgatg tcgtcggcaa gctgcgcctc gggccacagg atccggtcga tgccgaactg
     3121 ctcgcgcagc aggtcgcccc ccccgtcggg ctgcaggaag tcttccttcg atacgaggca
     3181 gatcgtcttc ggcgacccga cgcggttcgc cagcgcgcag gcgaccacgt tcacctcgtc
     3241 gaggccggtg cagccgacca gcaggtcaca gcgctcgatc cccgcccggc gcagcatcgc
     3301 ggtgctcgtt ccgcttccgt gcaaaaactc cacgtccagc gacgagaacc gctcgccgac
     3361 cgtgggatcg tggtcgatga cgacgacgtc gtgggcgtcc gagagcgccc gcgccagcgc
     3421 gtgaccgact tcgcctccgc cgatgatgat caggtgcatg ggacgccgcg ccgaggcttc
     3481 aggaagcttt gagctggtcg atcgcctggc gcgcccagtc ggggtcgttc aacatcgcgc
     3541 gcccgatccc gaccaggtcg gcccattcct cccggacgat ctcgtcggcc atcgcggcat
     3601 ccgagatgcc gccggtgacc agcacgggga cgccgacgcg cgaccggatt gcctgggcga
     3661 gcggcaggaa gtaggccgag cccttgccgg cgcccctcga tccctgcagg ccgccggaga
     3721 cgtcgatcag gttcacgctg ttgtccgcga ggcaggctgc caccgacgct ccgtcttcga
     3781 ttcccagccc gccgggcgtc tcgtcgtcgg cgccgagccg gacgaagacc gggaagtggg
     3841 cgcccacccg gcggcgcacg ctcgagacga cctcgcggtg caggcgggcg cgctgctcga
     3901 gggggccgcc gtagtcgtcg gttcgccggt tggtgagcgg cgacaggaac tgcgacagca
     3961 ggtacccgtg ggcggcgtgg atctggaccg cgtcgaaccc cgcggcggcc gcgcggcctg
     4021 ccgcctcccc gaaggcggca atcacttcct ggatctgcgg gaccttcatc tcctctggca
     4081 cgtcgccctc gggctcgtac gggtggcgga gggccgaggg cgccagcggg gcggcgccca
     4141 gcacacgccg cgagccgcgc gatccggcat gggcgagctg gaggcacacg gccgccccct
     4201 ccgccttcac cgccgcagca agccggccga gcccctcgat ggtggcatcg ttgtggacgc
     4261 ccatctgtcc ctgggaatgg cggccccgct cgtgcacgaa ggtgtgctcc acgatgatca
     4321 ggccgcagcc ggctgctgcc cggcggcgat ggtactccac gatcggcggg gtcacgaacc
     4381 cctccggcgt cgccagtccg gagcacatgg gcggcatgac gattcggttc cgcaggtgga
     4441 gggtcgtgaa ccgcagcggc gagagcaggg tcggcatggg agagaaatgt aaaagggaag
     4501 aggcagaatg taaaaggaaa acaaccgcgc gtcatccctt ccgcaatctg cctgcgccct
     4561 gctacgctcc cgccgccggg agagtcctgg accagcagcc tgggggctat gccggctcgc
     4621 cgaaccggag cgccatcatc gtcgcgtcgt cgagaggctc ggcgcggccg cggaacgccc
     4681 ggacgtcctg ctcgatgcgg ccgagcaggg agtcgacctc gcgcggaccg gcggcaacca
     4741 ggagcgactc gagccgctcg atgccccaga cgtcgcccgc ttcgttctcc gtctcgacca
     4801 tgccgtccgt gtaaaagaac agcaggtcgc ccggtgccag cgtgaccgag cgctcctggt
     4861 agccgtggcc ggcgaacagg ccgagcggca tgccggtcgt cgtgagccgg tcgagacctc
     4921 caggccgcag cacgaactgc gtgttgtggc cggcgttcac gtaccgcagc acgccccgcc
     4981 gcgggtcgag gatgccgacg aagagcgtga ggaagacttc cggaggcgtg tgggcctcca
     5041 ggtcggcgtc gagggcgtcg gccagcgcgg ccagatcctg ctcgaggggc agcctcgccc
     5101 gcagcgtcgc ctgcacgttc gccatcagca gcgccgcacc cacgcccttc ccggacacgt
     5161 cgccgaccag cagcgcgatg tctcccgacg gcaggacgaa gtagttgaag aagtcgcccc
     5221 ccacctcgcg cgccgggatc gagaccccct tcacctcggt gacgccgaga cggagcggct
     5281 cgcacgggag gatgccggtc tggatctggc ggcacagctc cagctcgcgg tgcagccgct
     5341 cctgctcgac gagcagcgcc tggtgcgcgg caagatcctg ggccatctgg ttgaaggcgc
     5401 ccgccagctc gccgaactcg tcgcgtgagc gcacggggat gcgcgtcgag aggtctccct
     5461 gcgcgatccg ctgcacgccc gccatcagcg agcgcagatg ccgggtcatg cccgaggcga
     5521 gcggcagcga acccgcgaag accagggcga tcaggccgaa ccccatgccg aagttcaggg
     5581 cggcggcccg ctgcagctcg cgcaggtcgt cggcgatggg ccgtgcaatc cccaccgtca
     5641 cgcccgtccg gtccttgtgg gtggccacca cccagtcgtc gatcgtgcgc gtggcggtgc
     5701 cgtcgacccg ggcgagcgat gccgggcgca gcgccgcgat ttgccggtcg cctccggcgg
     5761 gggtgtgcac ctggccatcg gcgtcgaccg caaacgggat ctctccctgg tgccgccgcg
     5821 tgttcgcgag gaccggcgcc agcatccgct tcgcgttgag ccgggccttg atgcgggcca
     5881 cctgctggcc gttctcctcg accgagcagc tcaaatcgtt ccccttgaac accgttaccg
     5941 tcttcacctt caccgcggca ggcttgggcg gccgggcggg cgccccccct gcgcccgccg
     6001 cagagcgctc gccacggcgt gccgcctcca ccgacaacgc ctgcaggtcg ccgacgccgc
     6061 gctggatctg catctggagg tcgcgcgccc aggcggcgaa ctcggcttgc ccggccgccg
     6121 aggcgcccgc ggcccggtac ttggccatgg cggtttcgat catcttcggg acctccaccg
     6181 tcaggcgtcc ggccccgtcc accgcgatca ggctgtcgcg ccccttggcg gcacgctccg
     6241 ccatcgcgtc caccttcccg gccgggggcg cggccgctgc ggcaggcgcc ggcggcgccg
     6301 gcggagcagc cggttcgggc gcgccgggca cctcgcgccc cccggtggcg atcgattcgg
     6361 ggacgatctc caggcgctcc agcatcggcg ccacctcgcc cagggtgctg accaactcgc
     6421 cggcaatcgc ctcggcgggc aaccgcttcg ccggccgggg cgtcagcgtg gcgatgtcgc
     6481 cctcggccac atggtcgtcg gccggccaca ggccttcggc cggggccgtc cacagcttgt
     6541 caatccgccg ctcgaggtcg cgggtcaccc agtccatccg ctgacccaac tcggccgcca
     6601 ggttcctcgc ctgctgctcg gcagcgtgcc ggagggccgc ccgcgacgtg ctgtaggagt
     6661 acagcgtgac gacggtgaga gggatgacgg agatggcgaa gaaggctagc gtcaggcggc
     6721 tgcggaggct catggtttcc tcgcggggtg ctgacaggat agacgatggg cgggggcggg
     6781 cggttctggc gatgattacg gttcttgcac ctcatccggc atttcggttg acgcgaaccg
     6841 gaccgcctgg cggcggccgc cggccgttgt gaggttttca agagaatttc agccctgcgc
     6901 gctggcgtgg ttgttgccga cgccctggcg ccatgctcga cttctgcccg cggtggatcg
     6961 ccgagcgcct cggccactcg gccgacccgg tctacttcgc cacctggcgc gtctcgggct
     7021 ggcagccggg gctcgagccg gccgagcgcg aggtcgtcgc ccaggtgctg cagctgcacc
     7081 gtgacgcgcg ctgcacgatg ctcgccttcg tcgtcatgga cgaccacgtg cacgtgctcc
     7141 tgcgttgccg gcgcggcagc gtggactcgg tgctcgaggc gatcaagacg tactcggcgc
     7201 accggctgcg cgatgtctac ggccggtcgg gtgcgatctg gcagcacgac tggttcgggc
     7261 ggaaggtcca gggcgaggag gatcttcgga gacagaccga gtacatcgtg tcaaacccct
     7321 ggaagcggtg gcccttcacc gaggggtatc cctgggtgtg gccctactcc tccctgaccc
     7381 acatccagcg gtagccatgc aggaacggcc agcgcttcca cgggttgccg acgattgaga
     7441 gctccacgtc acgacggcgc tgaggcgtgc gaacggcctg cgcctggtgg ccggcggccc
     7501 agatttcacc ccgccggccg tggatgcgtt gcagctgctg ctcggtggac gaccgccagg
     7561 agccgacgat ccgatcggtc ggccaggagc cggcacgagc gagggcatgg acgtgatcgt
     7621 ccatgacgac gaaggccagc agggtatagc ggaacccgtc gccgcgtcga aggacggagg
     7681 ccaccaggtc gcgctcccgc gaatcgagcc ggcgctgacc cggcgccagg cgccaggtga
     7741 tgaaggacgg atcgcccaca ccgggtccaa atcacctttc gtgccacgcc ggcacgggtg
     7801 acgggtgccc gccgcgccgg ttcgaaggcc cgggcgagtg caggtcctgc caggccggcg
     7861 ccgtccgaca tggcggaaac tgcgtccggg tgccggtccc gtctgctatc ctcggcggga
     7921 tgcggcgacc gaggcggcgc gggcagtgag tccgtgggag attggcggga ttgcggtggg
     7981 gctggcggcc gacgcgagcg ccgtcgccct ggcggcgggg tcgagcgccc ttgcgcgggg
     8041 cttccggttc cggttccgcc tggccttcca cttcggcctg ttccagttca tgatgccgct
     8101 ggcggggtgg ctggtcggcg cccgggtcgc ccgctacctc ggtgccgtcg atcactgggt
     8161 ggccttcggc atcctcgcgt acgtcggcgg ccgcatgatc gcggacggcc tgtcggcgaa
     8221 gccgcgaacc gcgccgcgtg atccgagccg gggcctgacg ctggtaatgc tgtcggttgc
     8281 aacgagcctg gacgcgttcg cggtgggctt gtcgctggcg atgatcgacg cgggcatctg
     8341 gtgggcgagc gtcacgatcg gcgccgttac gggcctgctt tcggcgatcg cgctgcgcgc
     8401 cggctcggtg ctcggggcgg ccgcaggccg gcggatggag gtggcgggcg gcctcgtgct
     8461 ggtggccgtt gggcttcgta tcctgggtca gcacctcatg gcctgagcgg tggcgtcgtt
     8521 ggggcggctt ggcatcggca cccggattga cacatgacga ccctcgtgga tcgcgtggcc
     8581 gcactggtgg aggcgttcga aggccgcccg acctgggacg agtacttcat ggccgcggcg
     8641 ttgttgatgg cctcgaggtc ggcctgcggc cgtcttcacg tggggtgtgt cctcgtgtcg
     8701 gcgggggcgt acccgcaccg ggtcatcgcg gcaggctaca acgggttcct tccagggtgc
     8761 gcgcaccagt cgcgcctgcg cgacggccac gagcaggcga cggtgcacgc cgagcagaac
     8821 gcaatcgcgg acgcggcgcg gcgcggcgtc agcgtgggcg gcgccaccgc ctacgtgacg
     8881 cattttccgt gcatcaactg tgcgaagatc ctggccgctg caggggtacg ggcggtaaag
     8941 taccaccacg actacgacca cgacccgctc gtggacgcgt tgtgcggcga ggccggcatc
     9001 ggcctcgtgc agttgtagaa ccggccgccg ccctggccag cagcgccctc tctcggcacc
     9061 tgccaggcag gttcccgtcg aggaaaccgc tcgttcgtcc ggccggacgt cagacgtggt
     9121 gtcagggagt attggctgtc gtgaaatgtg attaaatgac gacgcggggg agcgtcccct
     9181 cttgtgcaac cctgcccctt ggaagcgctg accatgcagt tgacccgcgc ggctgactac
     9241 ggcgttcgtg tcatgatgca cctcgtctgc caggcctcgg gcaccagggc gagcctgacc
     9301 gacctggccg aggccgccga cgtctccccg gcgttcctgt ccaaggtgct ccagcgtctc
     9361 gttcgcgccg gcctgctcgc ctcgcggcgt ggcaagcgcg gcggcttcga gatgctcgag
     9421 aaggggcgcc aggcgtccct gtacgacgtg ctcgaggcgc tcgacggcct gccgaccctc
     9481 aacgtgtgcc ttctcgattc tggcagctgc caccggagcc cctggtgtgc ggcccacacc
     9541 gtctggacgg gggcccagca gcgcctgctc cagacgctcc gggagacgcc gatcgagggg
     9601 ttggcgtggg acagccacac caggcggctg tcgatgctgc cgccatccgg cgccgcgcag
     9661 ggccagggcc cggtccgggc caacgggcac ggcgagggca acggctggtg cccggtcgag
     9721 agctagctcg catcacggct ttcccttgca tcgcgcggac aggacggtag aatcccgttc
     9781 atgtctcagc gctacctgct cggcctcgac atcggcagct ccttcgtaaa ggcatcgctc
     9841 ctcgacgtgg ctaccggccg cgccgtggcg gccgcctcgg ccccggcaag cgagatggcc
     9901 atcgccgccc cgcagccggg atgggccgaa caggatccgc gcgagtggtg gcggcacgga
     9961 gttgccgcca cccgcgaggt cctcacccgg gccggcgcac ctgcctccgc cgtggccggc
    10021 atcggcatct cctatcagat gcacggcctg gtgctcgtgg accgccgcgg ccagccgctc
    10081 cggccatcga tcatctggtg tgacagccgc gcggtgccgg tgggagaggc ggccttcgag
    10141 cgcatcgggc gcctgacctg cctggcccgt gtcctcaatt cccctggcaa cttcaccgcc
    10201 gccaagctcg cctgggtgcg ggcgaacgag cccgacgtgt ttgccgccgc ggacaaggcg
    10261 atgctgccgg gagattacgt cgccttccgg ctgaccggcc gcgccgccac caccgcctcg
    10321 ggcctgtcgg agggcgtgct gtgggacttc ctcgatcgac gggtctcggc cgacgttctc
    10381 ggggcgctcg acctgcccgg ccggctcttg ccggagctgg tgccgacctt cggcgagcag
    10441 ggcgtcgtga gcgccgaggc cgctgccgag accgggctgg ccgcgggcac tcccgtctct
    10501 taccgtgccg gcgatcagcc gaacaacgcc ttctcgctcc gggtcctcca cccgggcgag
    10561 gtggcggcga cggcgggcac ctccggcgtg gtgtacgcgg tcatcgacac ggcggcctgg
    10621 gacaacgagt cgcgcgtcaa caccttcctc cacgtgaccg accatccggg ggcgccgcgc
    10681 tacggcgttc tcctgtgcgt gaacggcacg ggcgcactca acagctggct gaagcggctg
    10741 ctcggcgggg agcaggtgtc ctacgacgag atgaaccgcc tggcggccga ggtgccgatc
    10801 ggcgccggcg gggtcgtggt gcttcccttc gggaacggcg cggagcgctc gctgggcaac
    10861 cgtgagccgg gagcctcggt ccaccacctc tcgctcgtcc gccacgggcg gccgcacgtg
    10921 ctgcgcgcgg cgcaggaagg catcgtgttc gcgctccacc acgggatggg gatcatgcgc
    10981 gagatggggc tgcaggcgac ggccgtgcgg gcgggccgcg ccaacatgtt cctgagtccg
    11041 gtgttcgcgc aggcgttcgc cgatctcagt ggtgccacgg tggagttgtt cgacaccgac
    11101 ggcgcgcagg gagccgcgcg cgccgccggt cttggggcgg gcgtgtttgc gaccgaagag
    11161 gaggcgttca acggcctccg ccccctcgac cgcctcgatc cggacgccgg gcgccaggcg
    11221 cagtacgtgg acgcgtacgc acggtggcag gcggcactga agaaagagct ggagcggggc
    11281 tgagccccgc tccagctcaa ggctcccggc tcaggccggg ttcaagtgcc ggccgggctg
    11341 cagcggcaac cgcagggctt tagccctgcg cggttgtctc ttcctcctcc aacagccgcc
    11401 gctgtccctg cagcgcctgg attggcgcga tgtcctgcac cacctcgagc gtgccgaggt
    11461 actcgccctt cgggctgcgg accgggaagt agcggatgtg caccttgcgg ccgcccatgt
    11521 cgatccagaa ctcggccacc ttgcggctcc ccgccttgaa gtcggcgagg atccggttca
    11581 ccaggtgcac gctcttctgc gggtggcagt tctgcacggc ggtcccgatg gcgccccggg
    11641 ttcgcgggaa gatcttctcg ccgccctcgt ggctgaaata ccggacgcgg tcgtccttgt
    11701 ccacgaacga cagttcgacc ggcagcgtgt tcaggatggc ggcgagcacg tcgcgctcga
    11761 cgccgaacga caggtccttc aactcgcccg cctcgatgat gtgttccgcg agcgcatagt
    11821 accggctccg cgtgtcgggg cccaacgccg cctcgacggc ctcgataccc gcaaccaccg
    11881 ccgcggcgtc ggcctcgctc atcacccgcc gcgccatcgg gtagaggatg tcgttctcct
    11941 tccagtagtg gtccttgagc agcgctgcgt actcgcctaa cagggctcgc agctcgggca
    12001 acacttcgtg gccgccgccg aggtacgcct cgccggccgc caccaggcga ggcagcagcg
    12061 acgtgccgcg gtcgtgctcg gccagcatga ccgccagcgg accgccggcc cgcggaatgc
    12121 cccgccgctc gatgagcggg aagaggtgct gctcctcctt cttgttgtgg caggcctcga
    12181 cgtagccttg gaagtactcg agggcgtcgc gcaggatcga gggggccggc ccgcccggcg
    12241 cctcgaacgt gcgctcaacc gcctcgagaa cccgctcggt ggtttcgtga tccgtcatca
    12301 acagctgcga ccagtccgac atcgaagcct ccgtaggagt gagtgcggcc gaggcgttgg
    12361 ggcgacccct tgtgctcgcc cctcaagcgt aggggcgacc ctcgtggtcg cccctcctgc
    12421 agtccagctc cgcagtgtac tccggccgtc cttcatgccg ctatgacctg gatcagccgc
    12481 gtcgcagcgc cggggccacc cgccgcgcgc cgtcctgtat aatcgcggct tctcgaggcc
    12541 gtccacctcg actgacgccg gcgccggtgc gtcgcggaaa ggaactgctc tggtgctggt
    12601 ctccttgtcc cctcgccccg ctcgaccatt cctggtatcc ctcgcgctgc tcctgctcgc
    12661 catcccggcg gcggcccagc aggccccggc cctctcggtg aacgcgggcg gcggcctgcc
    12721 ttcccccggg gtgctgccgg cccgcccggt tcccgggctg gtgctggccg aggtccacct
    12781 gcgcgatctg ccgagcgccg acacgatctg gtcggtgatc gagaccgccg acccgttcgt
    12841 ggtggtggat cgcatcgcga acggtggcct ttacctcggc gagcacgagt ggttcgggag
    12901 ccacggcagt tcgggcacgc agtcgagctt cctgatggac ggcctcgacc tcaccgatcc
    12961 cgagcggacc ggcaccccgc tgatgcagct ggaccgggag tactttcagg cggtgggtgt
    13021 cgcgtactcc ttcctcgccc ccgagctggg aggggcgggc accacgctgg tgctggtccc
    13081 gcgcgccgcg ggcagccgct gggccggcca gcttcgcgcc gggtacttgc cgcaccagct
    13141 gcaggccgag aacgcgcgcg acggggcgcc gtccatcgcg cggtttggga cgacgggcgg
    13201 cgcgcaggtt cgtgtcggcg gcccggttgc cgagcggttg ggcgtcttcc ttaccggtcg
    13261 cggcgtcgcg tcgcggcgct tcgagcgagg ggaccagacg ccgctcgaca gccgcctggt
    13321 atcggggttc cttcacgcca cggcctcgct ctccgagaat catcgcctcc gggtcgtggc
    13381 ggccgccgat cgggcggcgc atccgttcta cgcccgtgcg cggttcccgc tcgccaccgt
    13441 gcgtgcgacc gacaactcgc gccacctcca cgccacctac gagcgcgtga cgccgcgcgg
    13501 caccgccttc agcgccgccg ccggatatca gcgaagcgag acgggcggcc cttccgacgg
    13561 ccacggcacc gtgacaggcg tgatcgagcg catcctcgat ggcccggtcc ccgagctggt
    13621 ggtcccgccg gtgggtcccc gggggcgcta cggcgcagtt ttccagctga atcccggttt
    13681 cggcctcgac cgcctcggcc gcttgtcggc cggcctctcg ctgaaccacg ccttctcaca
    13741 gcccgccatc caggcgcgcg cgctggtcgg cgagctggtg gacggcctgc ctgcccgcgt
    13801 ctgggacttc gagagcggct cgggcgaggt gcggcggcat gcgaccgagc tggcggcctg
    13861 ggtgaacggc caggttgcgc ttggttcgcg cctcgcgctc gatctcggcg cccgcctcga
    13921 ctggaccgag gcccgctcgg aagggcgcga cgagatgatt cgctggcgca ccgtcctccc
    13981 gcgcggcttg ctgcagtggc agattaccga cggcggcggc atcaacgcct tcctcgggta
    14041 cggcctctac cgccaccgcc tgccgctcgg gtacctggcc tacggcgacc ccggtgctcc
    14101 ggcgggtctc gtctaccggt gggacgaccg aaacggcgac cggtggcttc agcccggaga
    14161 ggtgggccgc ctggtggcgg cggccggccc gggcgcccgc tcctacttcg agagccggat
    14221 cgacccggat ctcgaggctc cgatcgcacg gcagctggtg cttggcttcg atctgcggat
    14281 gggcgagcgc tggcggtgga agacgaccgg cttcgagcgg cgtgacgtcg gactggtcgc
    14341 cccggtcaac gagggggtga cgctggccga ctaccggccg atcgacatcg aggaccgcgg
    14401 ggccgacttc ctcaatccca tcgacgaccg gctgctcacc gtttacgacc ggctgccctc
    14461 gtcgttcgga cgcgaccggg acgtgctcac caacccggac ggccacgacg cccactacat
    14521 cggcgtggac gtcgcgctcg agcgcgtctt cgacggccgg tggcacatgc tgttcggcgc
    14581 cgcggcgcac cggtcggacg gcctggccgg caaccgcggc ttccacgtga acgagaacga
    14641 cgtcggcgtg ctgggagagg cgttccagaa cccgaacgcg ctcacctaca cgcggggccg
    14701 cctgttcttc gagcgtggct acatcatcaa gtggtcggga ggggtcgtca ccgccagcgg
    14761 gctgcacctc ggggcggtgg cgcgctacca ggacggccag catttcgccc gcatggtcat
    14821 cgttcccgac ctcgcgcagg gccccgaggc cgtccaggcc tacacgcgcg ggcacagccg
    14881 cttcaccttc accttcaccc tcgacgcgcg gatcgagcag gcgttccggg tgggcggcgc
    14941 ccggctcggc ctgatggtcg aggcgttcaa cctgctgaac accgccaacg aggtggagga
    15001 ggacccggtc accacgcgcc ttttccgggc gagcaccgtc gtgcagcccc cgcgcgcgat
    15061 ccgcttcggc ctccgctacg atttctgagg gaccgctgtc ctgagctact tcgcgccggc
    15121 cgcgagccgc tcgcgcacct tctgcacttc gcggtggaag acgaagagcg gatcggcgct
    15181 ctcggtgtgc gtggtgaagt tccaggtctt gccgccgcga cgcttcaccc gaaggttctt
    15241 cttcaggtag tccacctcga agctctcgag ctgatcgaag gaaagggaga aggcgtcgtt
    15301 cttctgcgtg gtctcgtacc tgagcccccg cggcgttgcg accagccgcc cctcgcacga
    15361 gccgacgccg tgcttgtgca cgacgtccag cgcttcgtcc agccggatct ccttgaaccg
    15421 caccatcacg tcggatggtc ccggctccac ctcgatggtg cgcgactggc cctcgtagcc
    15481 gtcggccgag aggttcagcg tgtgggttcc gggcgcgacg ccgcgcgcgg tgaccggcgt
    15541 ctcgccgagg tactgccgat cgaggaacac catcgccccc ggcacgtcgc tgtcgatgcg
    15601 aagctcgccg agcgtggggg gcgccggttc gggcgccggc ttcggcgcct ctggcgccct
    15661 tctccgggcc cgcgggcgct cggacggctc ttcggccggc ttcccgggaa ccggctcggg
    15721 aacggtggcc gcggagggct ggggcgccgg gcgggccggc gactcggcct cgggctgtgc
    15781 gggcggccga tcgcccccgc cgaccgccca gagcagcagg ccaaccagca gcaccacgcc
    15841 accgagggcg gccatgacga cgaccaaccg cgacacgcga ggctcgtgtt cgtccatagg
    15901 tgtggttgaa cggcctgttc actcccactc gatcgtcccg ggcggtttcg tgctcacgtc
    15961 gatcgccagg cccgccacac cggggagggc gaggatcgac cgctgccact cgtccagcag
    16021 cgtctcgggc aggcgagccg gcgttgcggt catcgctcgc tcggagtgga tgggccggac
    16081 gacgatcagc tcgcgcccct gcccgtcgag cgccagcggc accagcacgg tcgggcactg
    16141 ccagatccga tcgtaggagc cggagcggcg caggccgtcc atcacgatcg cgtccgcctc
    16201 gcgaagcagg tcgagccgct cgcgcgtgac gccggccggg accggccgtg cctcgcgagg
    16261 caggcgaggc ccgacgttcc agacgcaccg gttgacgccc ggcacgtgcc ggaagatcat
    16321 cccggcggcc tcgagcacgc gctcccaccc ggggtcgccg tggaggagca ccgggtgctc
    16381 gtacgaccgc aggtcggcct tcaccccgac cgaccggatc ggcagggcca gcgtggagag
    16441 cccgtactcc cccgccaccc gcgccgcggc cggctcgatc tcgccgaagc cctcgcggtc
    16501 ttcgatgccg tccgagcaga gcagccgcac gccgaggccg ggcccgggga aggggtgcct
    16561 ccacagcagc tcgcggggaa gtcccagctg ttcgcccagc tcgcgaacct ccaccttgta
    16621 cagctcggcg agcggctcga ccactttccc cgccgcgatc atctcctcga cgatgggcac
    16681 ccggttgtgg tgggtcttga tggtgtcggc gcgcttggtc ccgcccgttt cgatcgtgtc
    16741 ggggtagatg gtcccctggc ccagcaggtg gtcttcgatc ccgaggcgcc tcgcctcgcg
    16801 ttcgaagacc tcgatgaagg tctccccgat gatcgcgcgc ttccgctcgg gcgcgacgac
    16861 gccggcgagc gcgcgcagga agtcgtcgct ggcgtccacg aagtggacgt gacggccgag
    16921 gccgagcgcc cgcagcatct ccacgacctt gcggctctcg tccttccgca tcaggccgtt
    16981 gtccacgtgc agcaggtgca ggcggtcggc gccgacggcc atcgcgaaca gcttcgcggc
    17041 gaccgtcgag tccacgccgc ccgaggcgag caggaacacc gagcggtcgc ccacctggcg
    17101 ccgcacgcgc tcgacctgct cctcgaggta ccgctccatc gtccacgacg ggcggcagcc
    17161 gcagatgcgc agcacgaagt tggcgatcat ctcgtcgccg tgctcggtgt cgtccacctc
    17221 cgggtggaac tggacgccgt agcggcggtg ggtgtccgag ccgatcgccg cgaagcggtg
    17281 gccctcgccg ccctcgccga gcgcggtgta gcccagctcc tcgaaccccg ggcctaccga
    17341 gacgaccgag tcgaagtggc tcatccacac ccgctcgatc ttgccgaggc ccgcaaacag
    17401 ggggtggtcg tcccgcacca ggtgcaggga ggccgggccc cactcgcggc cgccgtgaac
    17461 cacctggccg ccgtagtgct tcgcgatttc ctggtgaccg aagcagaacc cgacgatcgg
    17521 cacgtcgagg tcgtagatgc ccttcgtgta ctcggagtcc tcgccgaacg acgacagcgc
    17581 cgggctcccc gacaggatga tgcccctgta cttgcggaac gcctcgagcg gatcctccgg
    17641 ctgtcggatc tcggcgagca cgtgcaggcg ccggaccttg gtggcgatca gatgggcgta
    17701 ctgcccgccg aaatcaacca ccgcgatcgc gtcgtggacc gcctcgtgct cttcagccat
    17761 acgcc
//
LOCUS       DAOPQX010000032        17648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3830_length_17648_cov_4.320241, whole genome shotgun sequence.
ACCESSION   DAOPQX010000032 DAOPQX010000000
VERSION     DAOPQX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17648
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3830_length_17648_cov_4.320241"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKK95_03100"
     CDS             <1..395
                     /locus_tag="PKK95_03100"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOC17225.1"
                     /translation="AALLDWLMLAAIDLVVVYFTLRICQLTVAEIGRLPVAPMALFFV
                     LLNGGYLALLTGAGGQTIGKMAFELKVVGIHGEAVPMGQAMLRTLALLASAVPAGLGL
                     LSVVIDGSRRGLHDRISRTRVVVHEARS"
     gene            447..911
                     /locus_tag="PKK95_03105"
     CDS             447..911
                     /locus_tag="PKK95_03105"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOC17226.1"
                     /translation="MRRLAIFLATAGYSGYFPFAPGTVGSAVGLVLFAALRWFATPLA
                     EVAAVVAISLAGVWAAHEAELHWGRRDPGAIVIDEVAGMLVTLLAVPVSWAGALLGFL
                     LFRAYDIAKPFGARQFERLPGGYGVMADDLVAGLYANLTLRAALWLLPALSA"
     gene            946..2217
                     /locus_tag="PKK95_03110"
     CDS             946..2217
                     /locus_tag="PKK95_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOC17227.1"
                     /translation="MHGATAPRRAAILAVGSELLTPFRTDSNSLAITSALNDLGIRVV
                     WKAIVGDQQDELAGAVRHALSLADLLVLTGGLGPTDDDVTREAVAEALGLPLEEHEAI
                     AEGLRARFAARGLEMPAINRRQAKVIAGATILPNANGTAPGQWIAAGGRYVLLLPGPP
                     REMRPMLAAVLAAKVAPLVKGARVMRRVVKIAGRPESWVEERAHQLYGAWRERVPPIE
                     ESTLASPGQVELHLSVVAPSEAEGHAALQRAVDELLAVFEGEVFSTDGRSLEEVVGDL
                     LRRRGWRVALAESCTGGLACSRLTDVPGSSDYVERGFVTYSNRAKTELLGVGEELFAA
                     HGAVSEAVAEAMAAGARARADVEIAVGITGIAGPGGGSREKPVGTVAIAVAWPGGGCV
                     ERYRFTGDRQAVKFQASQAALTLLRKRLEQV"
     gene            2217..2822
                     /gene="thpR"
                     /locus_tag="PKK95_03115"
     CDS             2217..2822
                     /gene="thpR"
                     /locus_tag="PKK95_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOC17228.1"
                     /translation="MDESRGPRLFFAVELDEAVRRAAARVAAELSRALAADGRAEVSW
                     VRAENMHLTVRFLGETPAPRVAPLVEAFAVPLATPPFELRFAGLGTFPPSGPARVIWL
                     GIVAGHDQLAAVHREVEDRLGPLGVEREERPYRAHLTLGRVRGRLGPRAASIVGGTVA
                     AGVPASAAREVTLFESRLSPRGAIYTALCRTALGTARKEGR"
     gene            2819..3826
                     /locus_tag="PKK95_03120"
     CDS             2819..3826
                     /locus_tag="PKK95_03120"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOC17229.1"
                     /translation="MKPIGILGAGSWGTALAVHLARTGHQVRLWAREAEIVEQLRDRR
                     ENETFLPGIPVPPSVEPCGGFDRALDGAGLVLVAVPSHGVRDVLRGAAPLLPPGAVIV
                     SVTKGLEPGTLLRPSEVIAQEAGRGHPVVVLSGPSFAREVARELPTAVCAASEDLAAA
                     HLVQAEFRGRFFRLYTTPDVVGVEIGAALKNVIAIAAGVVEGLGLGHNAMAALITRGL
                     TEISRLASALGARRETLAGLTGLGDLVLTCTGGLSRNRQVGIELGRGRPLDDILGHMK
                     MVAEGVQTTAAALALGARVGIELPIAAQMAEVLSGRKSPRAAAEELMLRPQKGEPEGG
                     W"
     gene            3836..4774
                     /gene="ftsY"
                     /locus_tag="PKK95_03125"
     CDS             3836..4774
                     /gene="ftsY"
                     /locus_tag="PKK95_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOC17230.1"
                     /translation="MGLFDRLREGLTRTKQQLVDRFEDIVRHADAPERRGREVDVETL
                     EALEELLITADVGVAATERIVSAVKGRSRERASLRDLVKDEIRAVFAAADAASPGSGQ
                     AGFAPPRVVLVVGVNGTGKTTTVGKLANLLKSQGARPLICAADTFRAAAVEQLAIWAE
                     RAGVDIIRAREGADPAAVVYDAIASGKARGRDPILVDTAGRIHTRVNLMNELEKIRRV
                     AAREVTGAPHEVLLVLDATVGQNGLSQARQFTEVAGVNGVVLAKLDGTAKGGVAVAIA
                     HDLKLPIRYAGVGEGIDDLVPFSAGEYVDALFEEKW"
     gene            complement(4812..5174)
                     /locus_tag="PKK95_03130"
     CDS             complement(4812..5174)
                     /locus_tag="PKK95_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC17231.1"
                     /translation="MTRWGDALVSLLQEQGMTQAELARRAGIHPETVSHILRGGHCST
                     DTLEKVAAALQVDLGELFGAPPDKKTATLQRDRLVSAVLRELSAAVSDAVVQELAQRQ
                     RRRLPRRKGTEVSLPFAE"
     gene            5308..6681
                     /gene="ffh"
                     /locus_tag="PKK95_03135"
     CDS             5308..6681
                     /gene="ffh"
                     /locus_tag="PKK95_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOC17232.1"
                     /translation="MAGWRRSPAMFDALSTRLQEVFKSLRGEARLTEATIEAALREIR
                     MALLEADVNFKVVKAFVDRVRDKATGQDVLRSLTPAQQVVRIVRDEMIALFGDAAGGL
                     QPTSRSPRAILLLGLQGSGKTTTAGKLGTWLTRQGRHPLLVSTDVRRPAAIQQLSVLG
                     RQANLRVFDPEGDLDPVRRATGAMAEAKNRGFDVVLVDTAGRLHIDDELMNELQAIKD
                     AVQPSDLLYVADAMTGQDAIKSAGEFNRRIGVTGVTLTKLDGDARGGAALSVVAVVGV
                     PIAFVGTGERLEDLEPFHADRLVSRLLGMGDVLSLIEKAEQAIDQEDAERLERKIRED
                     DFTLEDFRDQLRTIRKMGPLESILGMIPGMGNLKQLAENKPDEKQLSRVEAIINSMTP
                     AERADYRIVNGSRRKRIALGSGTSVEEVNRLLKQFQQMRKMLKSLGGMAGGGKGAVKR
                     LRRMGRF"
     gene            6713..6997
                     /gene="rpsP"
                     /locus_tag="PKK95_03140"
     CDS             6713..6997
                     /gene="rpsP"
                     /locus_tag="PKK95_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOC17233.1"
                     /translation="MLAIRLSRTGSKRQAHYRVVVCEARQATESRFLEILGHYNPRTQ
                     PATVKVDRERVEHWLKAGARPSQTVRTLLARHLTETPAPEAAPETGAAGQ"
     gene            6994..7245
                     /locus_tag="PKK95_03145"
     CDS             6994..7245
                     /locus_tag="PKK95_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOC17234.1"
                     /translation="MSTAREVVEVVARALVDDPEAVRVTETDHRGTAVIELFVAPDDL
                     GRVIGRQGRTASALRTLVAAAAEHSGVKATLEIREPSGR"
     gene            7242..7793
                     /gene="rimM"
                     /locus_tag="PKK95_03150"
     CDS             7242..7793
                     /gene="rimM"
                     /locus_tag="PKK95_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOC17235.1"
                     /translation="MNPAEARAAWDEYALVGRIARAHGNRGQVIVNPDTDFVEERFRV
                     GSILRTWRDGRIEELRVTAYRLHLGRPVIALEGVETMDDAEAMAGLELRIPAGELAPL
                     PEGTFYRHDLVGCRVETTGGQAVGEVAAVEGDMGASRLVLRTARGEVLIPLAVEICVR
                     IEPAARRIVINPPEGLLDVNRRP"
     gene            7790..8557
                     /gene="trmD"
                     /locus_tag="PKK95_03155"
     CDS             7790..8557
                     /gene="trmD"
                     /locus_tag="PKK95_03155"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOC17236.1"
                     /translation="MKFDIVTIFPRMFEGPTREGILGRAIERGLVDLRVHDLRDYTDD
                     RHRTVDDVAYGGGPGMVLKPAPLFRAVEAIREARGAPDAVVLTSPQGRRFSQAEAGRL
                     SALSHVVVLCGRYEGVDERVREELATEELSIGDYVLTGGELPALVVLDAVARLLPGAV
                     GDEQSVEQDSFRRGLLDYPQYTRPAEFRGRAVPAVLLSGHHAEVRRWRKRQALTRTLE
                     RRPELIEAAELDEEERAILRELLVARRKEIDDEREGD"
     gene            8538..8891
                     /gene="rplS"
                     /locus_tag="PKK95_03160"
     CDS             8538..8891
                     /gene="rplS"
                     /locus_tag="PKK95_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOC17237.1"
                     /translation="MSARAIDGIERGQLVERPQLRSGDTVRVHVKVREGDKERIQVFE
                     GIVIGMHRGGARATFTVRKVSFGQGVERIFPLHSPIIDKVDVVRSARVRRAKLYFLRA
                     LKGKAARMKEAKRTA"
     gene            8907..9575
                     /locus_tag="PKK95_03165"
     CDS             8907..9575
                     /locus_tag="PKK95_03165"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOC17238.1"
                     /translation="MARPRAARTLENALRRLGFHRIAGVDEVGRGCLAGPVMAGAVVL
                     DPLRHIPGLRDSKLLPPAERERLYGEIARRSLGWAVAAVEPDEIDRINIHQATLRAMR
                     EAVLALTPLPDLVLVDAFRIPNLFIPQRAVVHGDARCSAIAAGSIVAKVVRDRHMARL
                     HQADPRYGFLQHKGYATAEHLAALALHGYSPTHRRSFRPPSLFDLLDDPGVPPEALPS
                     VLKP"
     gene            10056..12131
                     /locus_tag="PKK95_03170"
     CDS             10056..12131
                     /locus_tag="PKK95_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC17239.1"
                     /translation="MAIDRDATLRKAEKFLRQGRLDNAIAEYLRVVEDQPRDWSTVNT
                     VGDLLVRAGQYESAIEHYTRIADHLFTEGFLPRAAAVYKKILKVKPDQEHALVRTAEI
                     FERQGLVVEAKKALTNIAERRLKRGDRRGASEIQVRIGVLDPTDLSAGLAAAKASGEL
                     GQTAAAVEKLVRLAGEFAQRGKLAESLHALDEAVRLDPAHPEARAALVARCIEGGDLE
                     RATRYAAQAPEFKAIAAELFGRGREDEGLLVLERALEHDPSDVDTRVQLVRTHLAKGH
                     VDRARACLSGDVTDPELLLSLAEIELTSGTPERGREVLERVLAAQPGRRDELVMLGCR
                     LCEQDREAGFQCVDLATDAAIAEHDWPAAASGLHEFVTRVPGHIPALMKLVEVCVDGG
                     LEATMYTAQAQLADAYLEAGRANEARVIAEDLVAREPWQSANIERFRRALVLLGDPDP
                     DGVIADRLSGDSPFTSSDFVLDFDLNDPGKGLEPEEIGQQAAPAPEPAESSAREAAGI
                     DLAAILAAPAAPAGEPEEAIEIDMSDALAGLDLADGQRAPSELERVFEDFRDGASRAG
                     GGDAAGRSLRQAIADLDAGRVDEAVRAAQKAVRSPRHRFEAAVLLARVHRDRGKTHEA
                     IEWFERAAQAHAPNAGAGRDLLYELGHLLAEAGETARALAVFLELQADAPDYRDVGAE
                     IERLTQKTA"
     gene            12254..12445
                     /locus_tag="PKK95_03175"
     CDS             12254..12445
                     /locus_tag="PKK95_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17240.1"
                     /translation="MGLLLVLAPWSPIWERNYFVEQVPWIAAVARNNFVRGAVTGLGI
                     VNLAAGLAELLALFAMRRR"
     gene            12576..13457
                     /locus_tag="PKK95_03180"
     CDS             12576..13457
                     /locus_tag="PKK95_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOC17241.1"
                     /translation="MADPNRHDFGASLREARERAGITLRAISANTKISVLTLEALERN
                     DVSRLPGGVFTRAFVRSYAKEIHLDPEETLNQFLERFPEVAGDADLHEPAAEGIEGEP
                     RTGGARVLRLAAWLVPVLAGVVYFAFVRPGLEAPPVARSTAASEQAPLPAPPPPPAPA
                     DTRPGGEAIDPPPTAASPAVQPAAAELATPTGGEVPNAPPAAVPDGALRVTLAPRGPC
                     WVSVRSGGQVVYSGLMQAGERRDLELRGDIAVTAGDAAALSLAINGEPARALGDTGKV
                     VTVRFSLETYRELLEAR"
     gene            13461..14369
                     /locus_tag="PKK95_03185"
     CDS             13461..14369
                     /locus_tag="PKK95_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17242.1"
                     /translation="MDPSYRTPLLDCFRRGEASREVRLLAAAGQLAPRAHEQLALLVR
                     LASDEDPEIRALTERTIRRLPPEPLAAFLARADVHADIRAFFAERGLAPKDAAPGGDT
                     SAPLLPSEDDGAADTTTPGEAPEAAAADAAPGEAPGAAREGTAAGDAGESRPGTAGEA
                     GERPRGAVQRLALMDVGQRVKVAMLGTREERNVLIRDSNRVVAAAVLSSPKLTESEVE
                     SIARMANVSDEVLRIVGTSRAWIKNYNVVSALTRNPKTPIAISLSLLSRLTGRDVKML
                     STDRNVPEPLRVAARKQAIIGDSRRQ"
     gene            complement(14424..16202)
                     /gene="polX"
                     /locus_tag="PKK95_03190"
     CDS             complement(14424..16202)
                     /gene="polX"
                     /locus_tag="PKK95_03190"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOC17243.1"
                     /translation="MDNLAIARVLGEIADLLEIKAENPFKIRAYRNAADAVAHAPDRI
                     EALDASELRKLPGIGKEIAAKIRELAETGRMQHHQELLAEFPVSLLELLRLQGVGPKT
                     AALLYQTLHLSTLDELEEAARSGRLRTLRGMGAKKEELILKALDERRQHGGRHLLART
                     RDLAAVLVAHLQPLAPDAEFVPVGSLRRGVETCGDLDILAIGGERALIRAFTSNPLAE
                     RVLGEGDTKGSVLLRGGYQADLRLVPRESRGAALQYFTGSKAHNIALRDRAIGRGYKL
                     NEYGLFDLATGLSVAGESEEGIYEALGLAWIPPELRENRGEIEAAEAGTLPRLIARSD
                     LRGDLHCHTTATDGRDTIEAMAAAARGAGLSYLAIADHTQSLTMVGGLDEAAVLDHAA
                     RIRAVNAVMEGIEVLAGIECDIRPDGTLDLAGECLAQLDLVVASVHSALDMDREKMTS
                     RVLRALASPHVDVLAHPTSRLLLRRAPSALDLDAVIDAAAREGVALEINSLADRLDLS
                     DLNARLARSRGAAIVIDSDAHGQAGFEALADGVRVARRAWLEAADVLNTLPFEQFRKR
                     LRRNRSSGRPLWSPGPSGSAWRRNRP"
     gene            16244..17389
                     /gene="nagZ"
                     /locus_tag="PKK95_03195"
     CDS             16244..17389
                     /gene="nagZ"
                     /locus_tag="PKK95_03195"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOC17244.1"
                     /translation="MPVAFHREIGQLALVGFDGYSVTPEVASLARQFDLGGVILFKRN
                     VESPEQVAEIAWRARELSRGVPLWVGVDQEGGRVARVRAPLTEWPPAETLGRAGSEDL
                     AGRFGAALALELSVLGFSIDFAPVLDVLTNPANPVINGGRAISGDPEVVARIGAAIVR
                     ALQAGGVAACGKHFPGHGDTSVDSHFDLPLVEHPPDRFHAIDFVPFRAVIEAGVAAIM
                     TGHLLVPAFDEERPATLSHAVVEGQLRGELGWDGLVFTDDMNMKAVAARFTPVGAAVT
                     AIAAGCDVLLLCGTDPAVQAEVLEGLIHAVEREELPVKRVEDAIARQRAAKARFAAMK
                     PSVDPVSGEVIVPPLHRDWRPLRPESLRRLIGRDEHQAIAGEMREYL"
     gene            17403..>17648
                     /locus_tag="PKK95_03200"
     CDS             17403..>17648
                     /locus_tag="PKK95_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOC17245.1"
                     /translation="MIKARAVRPGDRVAIVAPSSPFQVDELEAGLAEIRRLGFEPVYD
                     DRLFARSGYVAGEPAVRAGALLDALADPSVTCVLTARG"
BASE COUNT         2400 a         6163 c         6610 g         2475 t
ORIGIN      
        1 cggcggcgct gctcgattgg ctgatgctgg cggcgatcga cctggtggtc gtgtatttca
       61 cgctccgcat ctgccagctg acggttgccg agattgggcg gctgccggtg gcgccgatgg
      121 ccctcttctt cgtcctgctg aacggcggct acctggcgct gctgaccggc gccggcgggc
      181 agacgatcgg caagatggcc ttcgaactga aggtcgtcgg catccacggg gaggcggtgc
      241 cgatgggcca ggcgatgctg cgaacgctcg cgctgctcgc cagcgccgtg cccgccggcc
      301 tgggcctgtt gtcggtcgtg atcgacggca gccgccgtgg cctgcacgat cggatctccc
      361 gtacgcgcgt cgtcgtgcac gaggcccggt cttgaacgaa cctccgtccc tcctcgcagc
      421 cccttgacgc atcgccgccc gcacccatga gacgtctcgc catcttcctc gccaccgccg
      481 gctattccgg gtactttccc ttcgccccag gcaccgtcgg ctcggccgtc ggtctcgtcc
      541 tgttcgcggc gctccgctgg ttcgcgacac ccctggccga ggtcgccgcc gtcgtcgcga
      601 tctcgctggc tggcgtctgg gccgctcacg aggccgagct ccactggggc cgcagggatc
      661 ccggcgccat cgtcatcgac gaggtggcgg ggatgctggt cacgctcctc gccgtccccg
      721 tttcctgggc cggagcgctt ctcggcttcc tgctgttccg cgcctacgac atcgcgaaac
      781 cctttggggc tcgccagttc gagcggctgc ccggcgggta cggcgtgatg gccgacgacc
      841 tggtagccgg cctgtacgcg aacctcacgc tgcgggccgc cctctggctg ctgccggcgc
      901 tgtccgcgtg agaaaatggg gccggtgccc ctcttcggga gacccatgca cggagcgacc
      961 gccccgaggc gcgcagccat cctcgccgtg ggcagcgagc tgctgacccc cttccgcacc
     1021 gactcgaatt ccctggcgat cacctcggcc ctgaacgacc tcggcatccg cgtcgtctgg
     1081 aaggcgatcg tgggcgatca gcaggacgag ctggccggcg cggtgaggca cgccctgtcg
     1141 ctcgccgacc tgctggtgct caccgggggc ctcgggccga ccgacgacga cgtcacgcgc
     1201 gaggccgtgg ccgaggcgct tggcctgccg ctcgaggagc acgaggcgat cgcggagggg
     1261 ctgcgagccc ggttcgccgc gcgcggcctc gagatgccgg ccatcaaccg ccgccaggcc
     1321 aaggtgatcg cgggcgcgac gatcttgccg aacgccaacg gcaccgcccc cgggcagtgg
     1381 atcgcggccg gcggacggta cgtcctgctg ttgcccggcc cgccccgcga gatgcggccg
     1441 atgctcgccg ccgtgctcgc cgccaaggtg gcgccgctcg tgaagggggc gcgcgtcatg
     1501 cggcgggtgg tgaagatcgc cggccgcccc gagtcgtggg tcgaggagcg ggcgcaccag
     1561 ctgtatggcg cgtggcgcga acgcgtgccg ccgatcgagg agagcaccct cgcctcgccc
     1621 ggccaggtcg agctgcacct gtcggtcgtg gcgccttcgg aagccgaagg gcatgcggcg
     1681 ctgcaacggg cggtggacga gctgctcgcc gtcttcgagg gggaggtgtt cagcacggac
     1741 ggccgttcgc tggaggaggt cgtcggcgac ctgctacggc ggcgcggctg gcgtgttgcg
     1801 ctcgccgagt cgtgcaccgg cggcctcgcc tgctcacgcc tcaccgacgt gccgggcagc
     1861 tccgactacg tcgagcgggg cttcgtcacc tacagcaacc gggcgaagac cgagctgctc
     1921 ggcgtggggg aggagctgtt cgccgcgcac ggggccgtca gcgaggcggt ggccgaagcg
     1981 atggccgccg gtgcgcgcgc gagggcggac gtggagatcg ctgtcggcat cacggggatc
     2041 gcgggaccgg gcggcggcag ccgcgagaag cccgtcggca ccgtggccat cgccgtcgcc
     2101 tggccgggcg gcgggtgcgt cgagcgatac cgcttcaccg gcgatcggca ggccgtgaag
     2161 ttccaggcct cgcaggcggc cctgacgctg cttcggaaga ggctcgagca ggtgtgatgg
     2221 acgaatcgag aggacctcgg ctcttcttcg cggtcgagct ggacgaggcc gtgcgccgcg
     2281 ccgccgcccg tgtcgccgcg gagctgtcgc gggcgctcgc ggccgacggc cgcgccgagg
     2341 tgtcgtgggt tcgcgcggag aacatgcacc tcacggtccg gttcctcggc gagactccgg
     2401 cgccgcgcgt tgcgccgctc gtcgaggcct tcgcggtccc gctcgcgaca ccgcccttcg
     2461 agctgcgctt cgccggcctc ggcacgtttc cgccgagcgg ccccgcacgg gtgatctggc
     2521 tcggcatcgt ggcgggccat gaccaactgg ccgcggttca tcgcgaagtg gaggacaggc
     2581 tgggccccct cggcgtcgag cgcgaggagc ggccgtaccg ggcgcacctg acgcttggcc
     2641 gggtccgcgg ccggctgggg ccgcgggccg cttcgatcgt cggtggaacc gtggcggcgg
     2701 gtgtccccgc cagcgctgca cgcgaggtca cgctgttcga gagccggctc tcgccgcgcg
     2761 gggccatcta cacggcgctg tgccgcacgg cgctcggcac ggcgcgcaag gaaggccgat
     2821 gaagccgatt gggatcctcg gtgcggggag ctggggtaca gcgctcgccg tccacctcgc
     2881 gcggaccggt caccaggtgc gcctctgggc gcgagaggcg gaaatcgtcg agcagctgcg
     2941 cgatcgccgc gagaacgaga cgttcctgcc ggggattccg gtgccgccgt cggtcgagcc
     3001 gtgcggcggt ttcgaccgcg cgctcgacgg cgcgggcctc gtcctcgtgg cggtgccctc
     3061 gcacggcgtg cgggacgtgc ttcggggcgc cgccccgctc cttccgcccg gggcggtgat
     3121 cgtgagcgtg accaaggggc tcgagcccgg caccctgctg cgtccttcgg aagtcatcgc
     3181 gcaggaggcg ggacgaggtc acccggtggt ggtgctgtcg ggccccagct tcgcccgcga
     3241 ggtggcccgc gagctgccga cggcggtctg tgccgcgtcc gaggacctcg ccgcggccca
     3301 cctcgtgcag gcggagtttc gcggccgctt ctttcgcctc tacacgaccc ccgacgtggt
     3361 cggcgtggag atcggcgcgg cgctgaagaa cgtgatcgcg atcgcggcgg gcgtggtgga
     3421 agggctgggg cttgggcaca acgcgatggc ggctctcatc acgaggggcc tgacggagat
     3481 ctcgcggctc gccagcgcgc tcggcgcccg ccgcgagacc ctggcggggc tcaccggcct
     3541 cggcgacctg gtgctcacgt gcaccggggg gctgagccgc aaccgccagg tcgggatcga
     3601 gctcgggcgg ggccgcccgc tcgatgacat cctgggacac atgaagatgg tggccgaagg
     3661 tgtgcagacc acggcggccg cactggccct cggcgcgcgg gtcgggatcg agctgcccat
     3721 cgccgcgcag atggcggagg tcctctcggg gcggaagtcg ccgcgcgctg cggccgagga
     3781 gctgatgctc cgcccgcaga aaggcgagcc cgagggcgga tggtagaatc gcgggatggg
     3841 cttgttcgac cgcctgcgtg aaggcctcac ccgcaccaag cagcagctcg tcgatcgatt
     3901 cgaggacatc gtccggcacg ccgacgcccc cgagcgccgc gggcgcgagg tggacgtcga
     3961 aacgctcgag gcgctggagg agctgctcat caccgccgac gtcggcgtgg ccgcaaccga
     4021 gcggatcgtg agcgcggtca aggggcggag ccgcgagagg gcgagcctgc gcgacctggt
     4081 gaaggacgag atccgcgccg tcttcgccgc ggccgacgcc gcctcgccgg gcagcgggca
     4141 ggccggcttc gcgccgcccc gcgtcgtcct ggtggtcggc gtgaacggga ccgggaagac
     4201 caccacggtc ggcaagctgg cgaacctgct gaagtcgcag ggggcgcgac ccctgatctg
     4261 cgccgccgac accttccgcg ccgccgcggt ggagcagctc gcgatctggg ccgagcgggc
     4321 cggcgtggac atcatacggg cgcgcgaggg cgccgacccg gccgcggtcg tctacgacgc
     4381 gatcgcctcg ggcaaggcgc gggggcgcga tccaatcctc gtcgacaccg ccgggcgaat
     4441 ccacacgcgc gtcaacctga tgaacgagct ggagaagatc cggcgggttg cggcgcgcga
     4501 ggtgacgggc gccccgcacg aggtcctcct cgtcctcgac gccacggtcg ggcagaacgg
     4561 gctgtcccag gcgcgtcagt tcaccgaggt cgcgggcgtc aacggcgtcg tgctggccaa
     4621 gctcgatggc acggcgaagg ggggcgtggc ggtggcgatc gcccacgact tgaagctgcc
     4681 gattcggtac gcgggagtgg gcgagggcat cgacgacctg gtgccgttct cggccgggga
     4741 gtacgtggac gcgctgttcg aagagaagtg gtaaaacgct ggcgagggca cggcgtgcag
     4801 tgcccccacg gctactcggc gaacgggagg ctgacctccg tgcccttgcg gcgggggagg
     4861 cgtcgccgct gccgctgcgc cagctcctgc acgacggcgt ccgagacggc ggcggacagc
     4921 tcgcgcagca ccgcgctcac caggcgatcg cgctgaaggg tggcggtctt cttgtcgggc
     4981 ggggcgccga acagctcgcc gagatcgacc tggagggcgg cggcgacctt ctcgagcgtg
     5041 tcggtcgagc agtgtccgcc ccggaggatg tggctgaccg tctcggggtg gatcccggcc
     5101 cgccgggcca gttcagcctg cgtcatgccc tgttcctgga gcagcgacac cagcgcgtcg
     5161 ccccacctgg tcatggtgcc tcccaaccaa gacggttccg ccctgcgcat gctatgtggc
     5221 gcgggggccg agggcaaggg gtaagccggg ccgattgaca cccctgccag agccgttcta
     5281 cgataagggt ttgcctgcca tagctttatg gccggctgga gacggtcgcc cgcgatgttc
     5341 gacgccctga gcacacgcct gcaggaggtg ttcaagtccc tgaggggcga ggcacgcctg
     5401 accgaggcca ccatcgaggc cgcgctgcgc gagatccgca tggcgctgct cgaggccgac
     5461 gtcaacttca aggtggtcaa ggcgttcgtg gaccgcgtgc gcgacaaggc caccgggcag
     5521 gacgtgctgc ggagcctgac gccggcccag caagtcgtca ggatcgtgcg cgacgagatg
     5581 atcgcgctgt tcggcgacgc ggcgggcggt ctgcagccca cgtcgcggtc gccgcgggct
     5641 atcctgctgc tgggcctgca gggctcgggc aagacgacca cggccgggaa gctcgggacc
     5701 tggctgacgc ggcagggacg ccacccgctg ctggtctcga ccgacgtccg gaggccggcc
     5761 gccatccagc agctctcggt gctggggcgg caggcgaact tgcgcgtgtt cgacccggag
     5821 ggcgacctcg accccgtgcg gcgggcgacg ggcgcgatgg ccgaggcgaa gaaccgtggc
     5881 ttcgacgtgg tgctggtgga caccgccggg cgcctccaca tcgacgacga gctgatgaac
     5941 gagctgcagg cgatcaagga cgccgtgcag ccttccgacc tgctctacgt cgccgacgcc
     6001 atgaccgggc aggacgcgat caagagcgcc ggcgagttca accggcggat cggcgtcacc
     6061 ggcgtcacgc tcaccaagct cgacggcgac gcgcgcggcg gcgcggcgct ctcggtggtc
     6121 gcggtggtgg gcgtgccgat cgcgtttgtc ggcaccggcg agcggctcga ggacctcgag
     6181 ccgttccacg ccgaccggct cgtctcgcgc ctgctgggaa tgggcgacgt cttgtcgctc
     6241 atcgagaagg ccgagcaggc gatcgaccag gaggacgccg agcggctcga acggaagatc
     6301 cgcgaggacg acttcacgct cgaggatttc cgcgaccagc tgcggacgat ccgcaagatg
     6361 gggcccctcg agagcattct cgggatgatc ccggggatgg ggaacctcaa gcagctggcc
     6421 gagaacaagc cggacgagaa gcagctgtcg cgcgtcgagg cgatcatcaa ctcgatgacg
     6481 ccggccgagc gcgccgatta ccgcatcgtc aacggcagcc ggcgcaagcg gatcgcgctc
     6541 ggcagcggca cctcggtcga ggaagtgaac cggctgctga agcagttcca gcagatgcgc
     6601 aagatgctga agagcctcgg cggcatggct ggcggcggca agggcgcggt gaagcgcctg
     6661 cggcggatgg ggcggttctg acgggatggg gcgtctttcg aaagggtact cgatgttggc
     6721 gattcgcttg agccggacgg gatcgaagag gcaggcgcac taccgcgttg tcgtgtgcga
     6781 ggcgcggcag gcgaccgaga gcagattcct ggagatcctc ggtcactaca acccgcgcac
     6841 gcagccggcc accgtgaagg tcgaccgcga gcgcgtcgag cactggctga aggccggggc
     6901 gcggccgtcc cagacggtcc gcacgctgct ggcgcgccac ctgaccgaga ctcctgcccc
     6961 agaggcggcc ccggagacgg gggcggccgg gcagtgagca ccgctcgcga ggtggtggag
     7021 gtcgtggcgc gggcgctcgt ggacgacccc gaggcggtgc gcgtcactga gaccgaccac
     7081 cgcgggaccg cggtgatcga gctgttcgtt gcgcctgacg acctcggccg ggtgatcggg
     7141 cgccagggcc gtacggcctc ggccctgcgc accctggtcg ccgcggcggc cgagcactcc
     7201 ggcgtcaagg cgacgctcga gatccgcgag ccgagcgggc ggtgaacccc gcggaggccc
     7261 gcgcggcctg ggacgagtac gcgctggtcg gccggatcgc ccgcgcgcac ggcaaccgcg
     7321 ggcaggtgat cgtcaacccg gacaccgact ttgtcgagga gcggttccgg gtcgggtcga
     7381 tcctgcggac ctggcgcgac gggcggatcg aggaactgcg ggtgacggcc taccggctgc
     7441 acctcggccg gccggtcatc gctctcgagg gcgtcgagac gatggacgac gccgaggcga
     7501 tggcgggcct cgagctgcga atcccggccg gcgagctggc gccgctgccc gaggggacct
     7561 tctaccggca cgatctggtc ggctgccggg tcgagacgac cggggggcag gcggtgggag
     7621 aggtggccgc ggtcgagggc gacatgggcg ccagccgcct ggtgcttcga accgcgcgcg
     7681 gggaagtgct gattccgctc gcggtggaaa tctgcgtgcg gatcgagccg gccgcccggc
     7741 ggatcgtgat caatccgccc gagggcctgc tcgacgtgaa ccgacgcccg tgaagttcga
     7801 catcgtcacg atcttcccgc gcatgttcga gggccccacc cgcgagggga tcctcggccg
     7861 cgcgatcgag cgggggctgg tggacctccg ggtccacgac ctgcgcgact acacggacga
     7921 tcggcaccgg acggtggacg acgtcgccta cggcggcggg ccgggcatgg tgctgaagcc
     7981 cgcgccgctg ttccgggccg tggaggcgat ccgcgaggcg cgcggggcgc ccgacgccgt
     8041 ggtcctcacc tcgccgcagg gccgccgctt ctcgcaggcg gaggccgggc ggctgagcgc
     8101 gctctcgcac gtggtcgtgc tgtgcgggcg ctacgagggg gtggacgagc gggtccggga
     8161 agagctggcc accgaggagc tgtcgatcgg cgactacgtc ctcaccggag gcgagctgcc
     8221 ggcgctggtc gtgctcgacg cggtggccag gctgctgccc ggcgcggtcg gggacgaaca
     8281 gtcggtggag caggattcgt ttcggcgcgg gttgctcgac tacccgcagt acacgaggcc
     8341 ggcggagttc cgggggcgcg cggtgcccgc ggtgctcctc tcggggcacc acgccgaggt
     8401 tcggcggtgg cggaagcggc aggcgctgac acggacgctc gagcgccggc ccgagctgat
     8461 cgaggcggcg gaactggacg aggaagaacg ggccatcctg cgcgagctgc tggtggcgcg
     8521 acggaaggag atcgacgatg agcgcgaggg cgattgacgg gatcgagcgc ggccagctgg
     8581 tcgaacggcc gcagctgcgg tccggcgaca cggtgcgggt gcacgtgaag gtgcgcgagg
     8641 gcgacaagga gcgcatccag gtgttcgagg ggatcgtgat cggcatgcac cggggtggcg
     8701 cccgcgcgac cttcaccgtc cgcaaggtgt cgttcggcca gggcgtcgag cgcatcttcc
     8761 cgctgcactc gccgatcatc gacaaggtgg acgtggtgcg ctccgcccgg gtgcggcgcg
     8821 ccaagctgta cttcctgcgc gccctgaagg gcaaggcggc gcggatgaag gaggcgaagc
     8881 ggacggcgta ggttacactg ggggccatgg cccggccgcg cgccgcgcgc acgctcgaga
     8941 acgcccttcg ccgcctgggg ttccatcgga ttgccggcgt ggacgaggtg ggccgtggct
     9001 gcctggccgg ccccgtgatg gccggcgccg tcgtcctcga tccgctgcgg cacatccccg
     9061 ggctgcgcga ctcgaagctg ctgccccccg ccgagcgcga gcggctctac ggcgagatcg
     9121 cgcggcggtc gctcggctgg gcggtcgccg cggtcgagcc cgacgagatc gatcggatca
     9181 acatccacca ggcgacgctg agggcgatgc gcgaggcggt gctcgcgctc accccgctgc
     9241 ccgacctggt gctggtcgac gccttccgca ttccgaacct cttcatcccg cagcgggcgg
     9301 tcgtgcacgg cgacgcgcgg tgctcggcca tcgcggccgg ctcgatcgtg gccaaggtcg
     9361 tgcgagaccg ccacatggcg cggctccacc aggccgatcc ccgctacggc ttcctgcagc
     9421 acaagggata cgccacggcg gagcacctgg cggccctcgc gctgcacggc tattcgccga
     9481 ctcaccgccg ttccttccgc ccgccgtcgc tcttcgacct gctcgacgac ccgggcgtcc
     9541 cgcccgaggc gctgccttcg gtcctcaagc cgtaactgcc acggacgtac cacaaagatc
     9601 acaaaggtca cgaagaacac gaagcgtgta cccgtaggtt gccgttcttc ggcgccgcgc
     9661 agcggcgggc aggggccgcg ggcgggcgtc cgcaagccag catcgaccgc cggctggctc
     9721 gcggacaccc acccgcaccc ctgccgcgtc ggccaccctc cgggtggccg agcgcccgaa
     9781 gaccggtggg ccctccgcta acctttgtgt cctttgtgtt ctttggggtt tgaaccgttc
     9841 ttcgtgtcct tccttgtctg agccgtcttt tgtgtcgctc gtggtccggg acgccggaaa
     9901 agtggcctgt gtgaggccga attgtggccc tcccttcggg gtgccgtcac atgggctggc
     9961 ggacgcggcc ggatgaaccg atattctcaa gcgagccccg caggccgccg tcccaccggg
    10021 ttcgggtcat cggggagtga gttgggaggc aactggtggc catcgaccgc gacgccacgc
    10081 tgagaaaggc cgagaagttc ctccggcagg gcaggctcga caacgccatc gccgaatacc
    10141 tgcgcgtcgt ggaggatcag ccccgcgact ggagcaccgt caacaccgtc ggcgatctgc
    10201 tggtccgggc gggccagtac gagagcgcga tcgagcacta cacccgcatc gccgaccacc
    10261 tcttcaccga ggggttcctg ccgcgcgcgg ccgccgtcta caagaagatc ctgaaggtga
    10321 agccggacca ggagcacgcc ctggtccgga cggccgagat cttcgagcgc caggggctgg
    10381 tcgtcgaagc caagaaggcg ctgaccaaca tcgccgagcg ccggttgaag cgcggtgacc
    10441 gccgcggcgc ctcggagatc caggtccgca tcggcgtcct cgatcccacc gacctctcgg
    10501 ccggcctcgc ggccgcgaag gcctcgggcg agctggggca gaccgcggcg gcggtggaga
    10561 agctggtacg gctggccggc gagttcgcgc agcgcgggaa gctggccgag agcctgcacg
    10621 cgctcgacga ggcggtgcgc ctcgacccgg cccaccccga ggcgcgcgcc gcgctggtcg
    10681 cccgctgcat cgagggcggc gacctcgagc gcgcgacgcg atacgcggcg caggcccccg
    10741 agttcaaggc aattgccgcc gagctgttcg ggcgcggccg ggaggacgag gggctgctgg
    10801 tgctcgagcg cgcgctcgag cacgacccgt ccgacgtgga cacccgcgtg cagctggttc
    10861 gcacgcacct cgcgaagggg cacgtcgatc gcgcgcgcgc gtgcctgtcg ggcgacgtca
    10921 ccgatcccga gctgctgctc agcctggccg agatcgagct gacctcggga acgcccgagc
    10981 gcgggcgcga ggtgctcgag cgcgtgctgg ccgcccagcc gggccgccgc gacgagctgg
    11041 tgatgctcgg gtgccgcctg tgcgagcagg accgcgaggc gggcttccag tgcgtggacc
    11101 tcgcgaccga cgccgccatc gccgagcacg actggccggc ggcggcctcg gggctgcacg
    11161 agttcgtcac ccgggtgcct ggccacatcc cggccctcat gaagctcgtc gaggtctgcg
    11221 tggacggcgg cctcgaggcg acgatgtaca cggcgcaggc gcagctggcg gacgcctacc
    11281 tcgaggcggg gcgagcgaac gaggcgcgcg tgatcgccga agacctcgtg gcgcgcgagc
    11341 catggcagag cgccaacatc gagcggttcc gccgcgcgct ggtgctgctc ggcgaccccg
    11401 atcccgacgg cgtgattgcc gaccgcctct cgggcgactc gccgttcacg agcagcgact
    11461 tcgtcctcga cttcgacctc aacgatccgg gcaaggggct cgagcccgag gagatcgggc
    11521 agcaggcggc gccggcgccc gagcccgccg agagttcggc gcgcgaggcg gccggcatcg
    11581 atctcgcggc gatcctcgca gccccggcgg cgcccgcggg cgaaccggag gaggccatcg
    11641 agatcgacat gagcgacgcg ctcgcggggc tcgacctcgc ggatggccag cgggcaccct
    11701 cggagctgga gcgcgtcttc gaggacttcc gcgacggggc ctcgcgcgcc gggggcggcg
    11761 acgccgccgg ccgcagcctc cgccaggcga tcgctgacct cgacgccggc cgcgtggacg
    11821 aggcggtgcg cgcggcgcag aaggccgtcc ggtcgccccg gcatcgcttc gaagccgccg
    11881 tgctgctggc gcgggtccac cgcgaccgcg gcaagacgca cgaggcgatc gagtggttcg
    11941 agcgcgccgc ccaggcgcac gcgcccaacg ccggcgccgg gcgcgacctg ctctacgagc
    12001 tggggcacct gctggccgag gctggcgaga ccgcccgggc gctggccgtg ttcctcgagc
    12061 tgcaggccga cgcccccgat taccgggacg tgggcgccga gatcgagcga ctgacgcaga
    12121 agacggccta gcggccgcgg ggcggacggc ccgatcgctg atcccggtcc gcccgccttg
    12181 catacaatga gagcgtgacc cggggagcgc tccgctgctg acgcgcctga tcttcgtcgc
    12241 ctacttcctg gaggtggggc tgctgctggt ccttgccccg tggtcaccga tctgggagcg
    12301 gaactacttc gtggagcagg tcccctggat cgccgccgtc gcccgcaaca acttcgttcg
    12361 aggtgcggtg accgggctcg ggatcgtcaa cctggcggcg gggctcgccg agctgctcgc
    12421 gttgttcgcg atgaggcgcc ggtaggagcg cgcgggaggc cgcgcgctgg tttgacagcg
    12481 tgttctcgag cccctaacat gggcgcgcgt gcagaagcgc agggcccgtc ccgcgcggct
    12541 gcgccgcggc cggtcgttcc gtctcttcac tgctcatggc cgatcccaac cgccacgact
    12601 tcggcgcctc cctgcgcgag gcgcgggagc gtgccggcat caccctccgc gcgatctcgg
    12661 cgaacaccaa gatctcggtc ctgacgctcg aggcgctgga gcgcaacgac gtgtcgcgtc
    12721 ttccgggcgg cgtcttcacg cgcgcgttcg tccgctcgta cgccaaggag atccacctcg
    12781 atcccgagga gacgctcaac cagttcctcg aacgcttccc cgaggtcgcc ggcgacgcgg
    12841 acctgcacga gcccgccgcc gaggggatcg agggggagcc gcgcaccggg ggcgcccgcg
    12901 tgcttcgcct cgccgcctgg ttggtgccgg tcctcgcggg cgtggtgtac ttcgcgttcg
    12961 tgcgtcccgg gctcgaggct cccccggtgg ccaggtccac cgctgcaagc gagcaggcgc
    13021 cgcttcccgc accgccgccc cctcccgcgc cggcggacac acggcccggc ggcgaagcga
    13081 tcgaccctcc gcccaccgcc gcgtcgccgg ccgtccagcc tgccgccgcc gagttggcca
    13141 cccccacggg cggcgaggtt cccaacgcgc cgccggccgc cgttcccgac ggggccctgc
    13201 gcgtcaccct cgcaccgagg ggcccctgct gggtgtcggt tcgatccggc ggccaggtcg
    13261 tctactccgg gctgatgcag gcaggggagc gccgcgatct cgagctgcgc ggcgacattg
    13321 ccgtgacggc cggggacgcc gccgccctct cgctcgcgat caacggcgag ccagcccgtg
    13381 cgctgggcga caccggcaag gtcgtgacgg tgcggttctc actcgagacc taccgcgagc
    13441 tgctcgaggc gaggtagcgc gtggacccct cctaccgcac gccgctgctc gactgcttcc
    13501 gccgggggga agcgtcgcgc gaggtccgcc tgctcgcggc ggcaggacag ctggcgccgc
    13561 gcgcccacga gcagctcgcg ctgctggtgc ggctcgcctc cgacgaagac cccgagatac
    13621 gggcgctgac cgaacgcacg atccgccggc tcccgcccga gcctttggcc gcctttctcg
    13681 cccgggccga cgtgcacgcc gacatccgcg cgtttttcgc cgagcgggga ctggcgccca
    13741 aagatgcggc ccccggcggc gacacctcgg caccgctgct gccgtcggag gacgacgggg
    13801 ctgcggatac cacgacgccg ggagaggcac cggaagcggc ggccgccgat gcggcaccgg
    13861 gcgaagcgcc cggggcggca cgcgagggaa cggcggcggg tgacgcgggg gagagccggc
    13921 ccggcacggc gggcgaagcc ggcgaacggc cccggggcgc cgtccagcgg ctggcgctga
    13981 tggacgttgg gcagcgggtg aaggtggcga tgctcgggac gcgcgaggag cgcaacgtcc
    14041 tgatccgcga ctcgaaccgg gtcgtcgccg ccgccgtgct cagcagcccg aagctgaccg
    14101 agagcgaggt ggagtcgatc gcccgcatgg caaacgtctc ggacgaggtg cttcggatcg
    14161 tcgggacgag ccgcgcctgg atcaagaact acaacgtcgt ctcggcgctc acccgcaacc
    14221 cgaagacgcc gatcgcgatc tcgctgtcgc tgctctcgcg cctcacgggc cgcgacgtca
    14281 agatgctctc caccgaccgc aacgtgccgg aacccctccg cgtcgccgcc cgcaagcagg
    14341 ccatcatcgg cgactcccgc agacagtagg ggcgaccctt gtggtcgccc tgacacgccg
    14401 ctgggcgacc acaagggtcg cccctacggc cggttgcggc gccacgcgct tcctgaaggg
    14461 ccgggcgacc acaagggtcg ccccgacgat cggttgcggc gcaggcgttt cctgaactgc
    14521 tcgaacggca gtgtgttcag cacgtcggcg gcctcgagcc aggcgcgccg cgccaccctc
    14581 acgccgtcgg cgagcgcctc gaagccggcc tggccgtggg cgtcggagtc gatgacgatc
    14641 gccgcgccgc gcgagcgggc gaggcgggcg ttgaggtcgc tgaggtcgag ccgatcggcg
    14701 agggagttga tttccagcgc gaccccttcg cgggcggccg cgtcgatcac ggcgtcgagg
    14761 tccagggccg agggtgcgcg gcgcaggagc agacggctgg tcgggtgcgc cagcacgtcc
    14821 acgtgaggcg acgcgagggc gcggagcacg cgcgaggtca tcttctcccg gtccatgtcg
    14881 agggcggagt gcaccgacgc gacgacgagg tcgagctgcg ccaggcactc gccggccaga
    14941 tccaacgtgc cgtctgggcg gatatcgcac tcgatcccgg ccagcacctc gatcccctcc
    15001 atcacggcgt tcaccgcccg gatgcgggcg gcgtggtcga gcaccgccgc ctcgtcgagg
    15061 ccgcccacca tggtgagcga ctgcgtgtga tcggcgatcg ccaggtacga gaggcccgcg
    15121 ccgcgggcgg ctgccgccat cgcctcgatc gtgtcgcggc cgtcggtcgc cgtcgtgtgg
    15181 cagtggaggt cgccccgcag gtcggagcgg gcgatcaggc gcggcagcgt ccccgcctct
    15241 gcggcctcga tctccccgcg gttctcgcgc agctcgggcg ggatccaggc gaggccgagc
    15301 gcctcgtaga ttccctcctc gctctcgccg gcgacgctca ggccggtggc caggtcgaag
    15361 aggccgtact cgttcagctt gtagccacgc ccgatggccc ggtcgcgcag cgcgatgttg
    15421 tgcgccttgg agccggtgaa gtactgcagc gcggcgcccc ggctctcgcg ggggaccagc
    15481 cgcaggtcgg cctggtagcc cccgcgcagc aggacgctgc ccttcgtgtc gccctcgccg
    15541 agcacccgct cggcgagggg gttggaagtg aaggcgcgga tgagcgcgcg ttcgccgccg
    15601 atggcgagga tgtccaggtc accgcaggtt tcgacaccgc gccgcaggct gccgaccggg
    15661 acgaactcgg cgtcgggcgc cagcggctgc aggtgggcga cgaggactgc agccagatcg
    15721 cgcgtgcgcg cgagcaggtg gcgcccgccg tgctggcgcc gctcgtcgag cgccttgagg
    15781 atcagctcct ccttcttggc ccccatgccc cgcagcgtgc gcagccggcc cgaccgcgcc
    15841 gcctcctcca gctcgtcgag cgtcgagagg tgcagggtct ggtagagcag ggcggcggtc
    15901 ttcgggccca ccccctggag ccgcagcagt tcgagcagcg agacggggaa ctcggccagc
    15961 agttcctggt ggtgctgcat ccggcccgtc tctgccagct cgcggatctt ggccgcgatc
    16021 tccttgccga tgccgggcag cttgcgcagc tcggaagcgt cgagcgcctc gatccgatcg
    16081 ggcgcgtgcg cgacggcgtc ggccgcgttg cggtacgcgc ggatcttgaa cgggttctcg
    16141 gccttgatct cgagcaggtc ggcgatctca ccgagcaccc gcgcaatcgc caggttatcc
    16201 aacgtggtgc accaccacaa ttataatggc tcgctgaggt gaggtgcccg tggcgttcca
    16261 ccgcgagatc ggacagttgg cgctcgtcgg cttcgacggc tactcggtga cgcccgaggt
    16321 ggcctcgctg gcacggcagt tcgacctggg cggcgtgatc ctgttcaagc gcaacgtcga
    16381 gtcgcccgaa caggtggccg agatcgcgtg gcgagcgcgg gagctgtcgc gcggcgtccc
    16441 gctttgggtc ggggtggacc aggaaggggg acgcgtcgcg cgggttcgcg cgccgctcac
    16501 cgagtggccg ccggccgaaa cgctcggacg cgccggcagc gaggacctgg ctggccgctt
    16561 cggggcggcg ctcgcgctcg agttgtcggt gctcggcttc tcgatcgact tcgccccggt
    16621 gctcgacgtc ctcaccaacc cggccaaccc cgtgatcaac ggcgggcgcg caatctcggg
    16681 ggatcccgag gtggtggccc gcatcggcgc cgcgatcgta cgcgcgctcc aggcgggagg
    16741 cgtcgccgcc tgcggcaagc acttccccgg gcatggcgac acgtcggtgg attcgcactt
    16801 cgacttgccg ctggtcgagc atccgcccga ccggttccac gcgatcgact tcgtcccgtt
    16861 ccgcgcggtc atcgaggcgg gcgtagccgc gatcatgacc gggcacctgc tcgtccccgc
    16921 cttcgacgag gagcgcccgg ccacgctgtc gcacgccgtg gtcgaggggc agctgcgcgg
    16981 cgagctgggg tgggacggcc tcgtcttcac cgacgacatg aacatgaagg ccgtggccgc
    17041 ccgcttcacg ccggtggggg cggccgtgac tgccatcgcc gccgggtgcg acgtgctgct
    17101 gctgtgcggc accgacccgg ccgtccaggc cgaggtgctc gaaggactga tccacgcggt
    17161 cgagcgcgag gaactcccgg tgaagcgggt cgaggacgcc atcgcccgcc agcgggccgc
    17221 gaaggcgcgg ttcgctgcga tgaagccctc tgtcgatcct gtttccggcg aggtgatcgt
    17281 gccgccgctg caccgcgact ggcgacccct ccggcccgag tcgcttcgac ggttgatcgg
    17341 ccgcgacgaa caccaggcga tcgccggcga gatgcgggag tacctgtgag ccggggccgg
    17401 agatgatcaa ggctcgcgcg gtgcggcccg gcgaccgggt ggcgatcgtc gccccctcca
    17461 gccccttcca ggtggacgag ctcgaggccg ggctcgccga gatccggcgc ctcgggttcg
    17521 agccggtcta cgacgaccgc ctcttcgcgc ggagcggtta cgtggcgggt gagcctgccg
    17581 tcagggcggg cgcgctgctc gacgcgctgg ccgacccgtc ggtgacctgc gtgctgaccg
    17641 cccgtgga
//
LOCUS       DAOPQX010000033        17613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3842_length_17613_cov_4.569541, whole genome shotgun sequence.
ACCESSION   DAOPQX010000033 DAOPQX010000000
VERSION     DAOPQX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17613
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3842_length_17613_cov_4.569541"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..633)
                     /locus_tag="PKK95_03205"
     CDS             complement(118..633)
                     /locus_tag="PKK95_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17246.1"
                     /translation="MVIDTTTPTALPDETRLRSVLDLLPSLRALPGVRAAAAAQKLPL
                     RGSGDNWGIEVQGRPDLELTTTAFRIVTHDYFQALGARVLRGRGFLPTDRATTERVVV
                     INEALAAKYFSGEDPLGRILLTGFDDRGERIVGVVENMAEARLTDALPAATSRLGPST
                     SPSIGSRPRAT"
     gene            complement(718..2262)
                     /locus_tag="PKK95_03210"
     CDS             complement(718..2262)
                     /locus_tag="PKK95_03210"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOC17247.1"
                     /translation="MRYITVVLVFGLLAAFAPAKPTPTRTIARTALDDKIRGGWAGKM
                     IGVSYGAPTEFQSNGRIFEGEIAWSPERVSNALRQDDLYVGMTLAETMDRLGFDATVE
                     QYGEAFKDSKYQLWHANASARRLLNLGIKAPMSGHPKYNIHANDIDFQIEADFIGLMS
                     PALPRESNKYSERVGRVMNHGDGLYGGIFVNGMYTAAFFESDVRKVVEQGLACLPPKS
                     AYARLIRDLLGWAEENPDDWKKTWQLIQDTWDRNDSCPEGALRPFNIDARINGAYVAL
                     GLLYGRGDFARTLEISTRAGQDSDCNPSTAGGILGVMLGYSRISDQWKGGIPAIADEK
                     FDFTHSSFNDISRSTLERALQLVRRAGGQVTDTEVVIPLQAPEAAPFEQWDMGIPERR
                     VGIDDPAWRFKGFTRVAGSNGPARMRASGAGAEAVLTFTGAAVALVGDLTQEGGRADV
                     LLDGSPLHAADAFIVERTHDDALWHTYGLAQGPHTLRIVTRDDADPRSKGKALVIREA
                     VVYRKK"
     gene            complement(2291..3226)
                     /locus_tag="PKK95_03215"
     CDS             complement(2291..3226)
                     /locus_tag="PKK95_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOC17248.1"
                     /translation="MDGLHRVLLATVAGLLFTLPSLGGSQVAAPPGDDMSVIAPGAKL
                     EKLADGFAFTEGPTADPNGNVFFTDQPNDRILKWTVEGRLETFMQPAGRANGMYFDAR
                     GHLLACADEKTELWSIAPDGTHTVLAREFEGKPLNGPNDVWASPGGGIYFTDPFYKRD
                     WWTHEAPPQGTEQVYYLSPDRKTLRRVTTGLVKPNGIIGTPDGRTLFVADIRGNKTYA
                     FDIQPDGNLANQRLFCEQGSDGMTLDTEGRLYLTGKGVSVFDASGKKIEQIDVPEPWT
                     ANLCFGGRDHKTLFITASEGLYAIRLRTRGANAAK"
     gene            3300..4214
                     /locus_tag="PKK95_03220"
     CDS             3300..4214
                     /locus_tag="PKK95_03220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC17249.1"
                     /translation="MKLQRREFLHRAALGAGGLLVGSAGLSASGAGLRAAETAFDPYA
                     LVPLGKTGLRVSRFCLGTGMRGGNRESNHTRMGKAALERLIRESFDRGTRLFDLADLY
                     GTHPYVVPALKGIPRDRYRIVSKVWFMRGGLPESERPGADVTIARFLKELGTDHIDLV
                     LLHCVTSPKWPDDLRRQMDQLSERKLKGDIGALGVSCHSLAALEAAAAEPWVESVHAR
                     INPYGMSMDGPPEKVVPVLRKIHDAGKGVVGMKIVGEGRLRDDPERRAESVRYALTLG
                     CVDVLNVGCESIAEVDDLASMVRRAGGA"
     gene            complement(4268..5611)
                     /locus_tag="PKK95_03225"
     CDS             complement(4268..5611)
                     /locus_tag="PKK95_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HOC17250.1"
                     /translation="MLPLTSLLPLVLLFSGSSVPAHPAVPVWDIQVEAEDRVVQQQGT
                     QGAFATGRYRNLFREAGYSEEAIRRKIDKAFGQLFHGNPDSETVFYWSGENANGRLAY
                     LSDINNRDVRSEGMSYGMMIAVQLDKKAEFDALWNWSRTYMYHDDRAHPAYGFFSWSM
                     KTDGTPNSESPAPDGEEYYVMALYFASARWGDGSGIYAYRAMADRLLSDIKNRGVITG
                     PWASRRDRVETDGAQFNLEHKMVRFTPDNRRPDHTDPSYHLPAFYELWARWGPEADRP
                     FWAEAARVSRDFFQKATHPATGLAPEYANFDGTPVTASFNQRSGTFGPDAWRTAANWS
                     VDWSWWAADPRERELSDRIQAFFESKGIDSYGNRWTLDGSVQLEANHSTALVATNAVA
                     SLAATHPRARRFVEALWNAEIPTGRYRYYDGLWYLMGLLHCSGEYRVWTPAWGRE"
     gene            complement(5786..6337)
                     /locus_tag="PKK95_03230"
     CDS             complement(5786..6337)
                     /locus_tag="PKK95_03230"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOC17251.1"
                     /translation="MNILEAILSAQGGGATRQIGEQFGLGEDQVQSALSALVPALASG
                     FQRNMASEGGLESLLGALGGGRHQRYLDDPGALASPETITDGNGILGHVFGSKEVSRQ
                     VAARASAQTGIGEGILKKMLPVVAAMMMGSMSKRASQPSMPGGAGAGGDLLNMLAPML
                     DANRDGSVVDDVLGMVGKLFTRR"
     gene            6462..7208
                     /locus_tag="PKK95_03235"
     CDS             6462..7208
                     /locus_tag="PKK95_03235"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOC17252.1"
                     /translation="MKRSSSWLALAVALALLPAAAAAQDPPKEPPPPPLFEGTVDFSF
                     VSTTGNSSTQSIGVSGDTTYRPDGWEIRNKAAFVRAKTDEELSAQSLVYLFRAARKIT
                     PRLSGYGQFDYTRDRFAGIEHRNAVLGGLEYLLYNTAPHDLKILGGAGYVNEQRLSGD
                     DVSTGALDAGWAYKLKVSETAEFTDDFRWGLSFYESSDWRIGHVAALTTRVHSILSLK
                     VSHTTRFVNFPPPGFEKTDTITAVALVAKF"
     gene            complement(7224..7841)
                     /locus_tag="PKK95_03240"
     CDS             complement(7224..7841)
                     /locus_tag="PKK95_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOC17253.1"
                     /translation="MSEEFVDYYELLQLSPNADDDLIHRVFRHLAKKCHPDAGDGNAE
                     RFRLLVVAHATLTNPAARATYDARYQEYWNRKWRLASEASDGSAFAEDQATREKLLSL
                     FYVQRRRDMMSPGLGEYELARLLRIPVELVAFHTWYLREKGWVQRTESGQLAITATGV
                     DSVERSRLRLPADRLLESRGSAADAPAEEDDPPPPGQIEAHTPRQ"
     gene            complement(7882..8931)
                     /locus_tag="PKK95_03245"
     CDS             complement(7882..8931)
                     /locus_tag="PKK95_03245"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HOC17254.1"
                     /translation="MPAAALVLALALANGVPGPPPARDFAPNPPAGRAVAAPREAVGA
                     RPRHIVSLRLDNDIVAGTDKDYSSGFSLALSLDGRGPLGGIWSVFGGSPSRLVSSYEL
                     GHVISTPANILAPVPDPSDRPYAGMLYGALATQALSGDRLDGLKLLAGVVGPASLAEP
                     IQRAIHTVTLSDQARGWDYQLRNELLLNVFYEHRRRFTVHATPGGWTLQAIPRAGASA
                     GNLLVQAQAEAYVRFGRHLPDDFGSSPARGLGNVPLPARHAATGSPNGTGAYVFAGAG
                     VFFVARNLTLDGNTFSDGPRVQEKAHAVPAGEVGVSIRNSRFEATVSFVAWGREHAAQ
                     WRPCRYGSATLTYSY"
     gene            9009..9866
                     /locus_tag="PKK95_03250"
     CDS             9009..9866
                     /locus_tag="PKK95_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17255.1"
                     /translation="MAAAVLAALCLFAPGLALGQEGPAPSGSEEAAALQGAFPDATFD
                     AYFRQYLPLKNVYSPYYAWDAAMALDLTVFRKGQGAVDFGAVVQTAGTENLGSQVGVG
                     GTGYILRAGYLRSLSRAVTLSAGIAHLSSHLTRDLDDKTDEQRRKGMLIPDVQDPDEY
                     NVVYLKAHLAFERWPLEPELDVILEPVNFRFDFGYAPYARPLYLGTRLILWRGVRKTL
                     ALETQHEVGNNGFNNFCLLLELFPKAQHGGRFHLFVGGSPGRTMHVSPNVSALRDGVT
                     AGIRLRFRT"
     gene            9946..10446
                     /locus_tag="PKK95_03255"
     CDS             9946..10446
                     /locus_tag="PKK95_03255"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HOC17256.1"
                     /translation="MDPRYPIGRFDPEDATPLPEVIDRIARLPGQLADAARDLERRGN
                     LDVPYREGGWTARQVVHHLADSHLNSYTRFRLALTEERPMIKPYDEKRWAELPDAKSG
                     PIEPSLHLIEGLHARWVDLLRGMSAADFGRVFVHPERGEMSLERTARLYAWHCRHHLA
                     HLALIK"
     gene            complement(10537..11550)
                     /locus_tag="PKK95_03260"
     CDS             complement(10537..11550)
                     /locus_tag="PKK95_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOC17257.1"
                     /translation="MDTNILAVLLLVFVALAFDFINGFHDAANSIATVVSTRVLTPGK
                     AVVWAAFFNFIAAFTFGTAVAKTVGGGLVDLQVVNFVVIFAGLMGAITWDLLTWYYGL
                     PTSSSHALIGGYAGAAVARAGFEAILPGGWTLTLIFIVLAPLIGLVLGFLIMVAILWL
                     FRGTSPGQVDHWFRRLQLASAAAYSLGHGGNDAQKTMGIISGALVAGGFLQLENGQLP
                     IPIWVVLMAHAAIALGTLSGGWRIIHTMGSKITKLQPVGGFAAETAGAISLFGATHLG
                     VPVSTTHTITGAIIGVGSIKRLSAVRWGVATRIVWAWILTIPASALIAAAVYWGSAAL
                     VGR"
     gene            11642..12289
                     /locus_tag="PKK95_03265"
     CDS             11642..12289
                     /locus_tag="PKK95_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17258.1"
                     /translation="MPPALIFVVALVSIMAALLVVVWRKERARTEALRQVAATAGLMF
                     QPKGDLAALRALGDVPLFERGHSRRVRNLMTGRVGDDHLAVFDYRYSTGSGKNQRTRS
                     QTVVLLPSATRSLPDLQMAPENPVTRLAEKFGFQDIDIDSSPEFSRRYVVRGKDEQAI
                     RAALAARATSYFAAHEGWTVEVRSGTLAIYREDRRPKPQDLRAFIEEACTAARNL"
     gene            complement(12366..12902)
                     /locus_tag="PKK95_03270"
     CDS             complement(12366..12902)
                     /locus_tag="PKK95_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HOC17259.1"
                     /translation="MRYRTLAAVLLAVVAVVASGACDDDNDNPLSPSPPNNTFTFTST
                     LLPGNEVPPVTNADASASGTVTVRLDVTRDAAGSITGGSADFTVNLSGFPAGTVLTGA
                     HIHQAPAGQNAGIAVNTGLGNGEVTLGSGSGSFTKENVNVTASLANALVSGPSGFYFN
                     VHTTINPGGAVRGQLALQ"
     gene            complement(12932..13873)
                     /locus_tag="PKK95_03275"
     CDS             complement(12932..13873)
                     /locus_tag="PKK95_03275"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HOC17260.1"
                     /translation="MSAVALLACATCLSPGAVAPALAQADAPSAAKVAPLGSPAAPPP
                     DERAHQDEEDEVDSSLTEADFSVVTLPTTLQLPRFGFNFRLTHRFLANLRQGSFSDHL
                     EDLFGLDNGAVIALELRFAPLSNLQVVVHRSSQDKTLQFSGQYDAIRQGRAWPVSISP
                     IASIEGTNNFKGRGDTGGDGGHDHGVGGGAHRSPALGAVVSRTFSDRLALYAVPMWVH
                     DSARLPEGDRNTGFLGLGGRARLSRNVFVVAEVSPRIGGYAPGSPGYAFGIERRAGGH
                     MFLLTFTNSVGTTFGQMSQGGFPDTLYMGFNLGRKFY"
     gene            complement(13999..14562)
                     /locus_tag="PKK95_03280"
     CDS             complement(13999..14562)
                     /locus_tag="PKK95_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17261.1"
                     /translation="MKLPSFVRQMVAPGDLAREKHLKTFRAEIQAAAASGDRSRIETL
                     LDRPKQLGLDAEDAALECEHLAGLLAAADLRERLARGEPLEVIPTRHPAITGERCHFL
                     APASSPDGLMDQGGKLFLTDRRILYLGASKLEAEWRQVTEVRDQERDIFLTVQPLDLL
                     RVRFNSYVDALRAAEIARYLSRSPRNR"
     gene            complement(14684..15379)
                     /locus_tag="PKK95_03285"
     CDS             complement(14684..15379)
                     /locus_tag="PKK95_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17262.1"
                     /translation="MQVEPYGGGRLRAVRRGWFTVPSRLFLLSPASCSGRRAQQLLSP
                     SAAFALAVRLRSAGGAPIGEVFAFISQLYFRGKLAYARAFARVPSDESGSALADGVFV
                     ITPSRGVLAWDTAVDAAAIAAFAAVEVHHANRAYVFALEEGARRLAAFLGDGDQVVLL
                     GSIASEKYAAVLVEVLGPRLVFPREFVGRGDMSRGGLLLRSVRACRELDYIPLAGSPR
                     HGVRPPRLDPAGR"
     gene            15558..16799
                     /locus_tag="PKK95_03290"
     CDS             15558..16799
                     /locus_tag="PKK95_03290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOC17263.1"
                     /translation="MAKARREAGAIWRRDGWLIVGLVLAVLVGFEGSVRKAFQFARQV
                     ELSYGLALLPALFILCALLAFHQYTKRQELKRQAAAAAAEAAQVKQRLLELERLAAFG
                     HALAGCLGFDTLQQVVWRHLPGFIGRSEAWLLTYNEGTWRVLADTTGLESDRSSQLQA
                     IAVEALSRGSEATGCADGIECAGAVCFPMIVGGQVVGVLGLDDAPTATPGCRRAMSAV
                     ATMLGIAARNVQLFRDVRDDALHDRLTGCLGRAHGLETLSRELVRVARMRRPVSIVMF
                     DIDYFKAINDRYGHLVGDEVLSAVGERLRSLLRGSDTRCRYGGDEFLVILPETPAEGA
                     VHVAEWLRMQLAAVRVPSLADASTPLRVSLGVATTDGRLEVEEMIARADAALYEAKRC
                     GRNCVRSYKASEEPFELGEAV"
     gene            16936..>17613
                     /locus_tag="PKK95_03295"
     CDS             16936..>17613
                     /locus_tag="PKK95_03295"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HOC17264.1"
                     /translation="MPLTRLLYVALLSCLLGLAAASASAGLPSTGTPSPGTGRAAAPS
                     GARIEALLLPEGTAGAVKLDGEFTEGAWEKAPPTRGFLQRDPKEGAEPAFDTEIRVLY
                     DRDFLYVGVRAHDSAPGAIVGIRTRRDEESPSDWLRVMIDSYRDRRTAYEFAVNAAGV
                     KQDRYWFDDGNSDVSWDAVWDVSVRRDEQGWRAEFRIPFSQLRFAPGKGDAFGFAVVR
                     QIGRLNET"
BASE COUNT         2600 a         6114 c         6175 g         2724 t
ORIGIN      
        1 cctcccacag gcgctcccgc atcgcccggg tctcggccag ggcgcgggca ccgagcgggg
       61 tgaccgagaa cacccggcgc gacaccgatt cgccgcgcgg ggcggcgcgc gccgagatca
      121 cgtagccctt ggcctcgagc cgatcgaggg tgatgtagac ggacccaacc gtgacgtcgc
      181 ggccggcaag gcgtcggtca ggcgggcctc cgccatgttc tccaccacgc ccacgatgcg
      241 ctcacccctg tcgtcgaaac ccgtcaggag gatccgcccg aggggatcct cgccggagaa
      301 gtactttgcc gccagcgcct cgttgatcac cacgacgcgt tcggtcgtgg cgcggtcggt
      361 ggggaggaag ccgcgtcccc gcaggacgcg cgcgcccagc gcctggaagt agtcgtgggt
      421 cacgatccgg aaggctgtcg tggtgagctc gagatccggc ctgccctgga cttcgattcc
      481 ccaattgtcg cccgagccgc gcaggggcag cttctgcgcg gctgccgcgg cgcggacgcc
      541 gggcagcgcc cgcagcgacg gcagcaaatc gaggaccgag cgcagccgcg tctcgtccgg
      601 caacgcggtc ggcgtggtcg tgtcgatgac caccccgtca cgcgtggcga agcccgggtc
      661 gatggcgcga agattggcca ggctgcggtt tcgaaaggtt gccgggggtg ccggccccta
      721 cttcttccga tacacgaccg cctcccggat gacgagtgct ttccccttcg accgtgggtc
      781 ggcatcgtcg cgggtgacga tgcgcagcgt gtggggacct tgcgcgagcc cgtaggtgtg
      841 ccacagcgcg tcgtcgtggg tgcgctcgac gatgaaggcg tcggcggcgt ggagaggcga
      901 gccgtcgagg aggacgtcgg cgcggccccc ctcctgggtg aggtcgccaa ccaacgccac
      961 cgcggcgccc gtgaaggtga gcaccgcttc ggcgccggcg ccgctggcac gcatccgcgc
     1021 ggggccattg gagccggcga cccgcgtgaa gcccttgaag cgccaggccg ggtcgtcgat
     1081 gcccacgcgg cgttccggga ttcccatgtc ccactgctcg aacggcgccg cctcgggggc
     1141 ctggagcggg atgacgacct cggtgtcggt cacctggccg ccggcccgcc gcaccagctg
     1201 cagcgcccgc tcgagcgtcg agcgcgagat gtcgttgaac gacgagtgcg tgaagtcgaa
     1261 cttctcgtcc gcgatggcgg gaatgccgcc cttccactgg tccgagatgc gggagtagcc
     1321 gagcatcacg ccgaggatgc cgccggcggt cgaggggttg cagtcggagt cctggccggc
     1381 gcgcgtcgag atctcgagcg tccgcgcgaa gtcgccgcgg ccgtagagca gacccagcgc
     1441 gacgtaggcg ccgttgatcc tggcgtcgat gttgaagggc cgcagggcgc cctcggggca
     1501 gctgtcgttg cggtcccagg tgtcctggat cagttgccac gtcttcttcc agtcgtccgg
     1561 gttctcctcg gcccagccca gcaggtcgcg gatgaggcgg gcgtaggcgc ttttcggcgg
     1621 caggcaggcc aacccctgct cgaccacctt ccgcacgtcg ctctcgaaga aggcggccgt
     1681 gtacattccg ttgacgaaga tgcccccgta cagcccgtcg ccgtgattca tcacccggcc
     1741 gacccgctcg gagtacttgt tcgactcgcg gggcagcgcc gggctcatca ggccgatgaa
     1801 gtcggcttcg atctggaagt cgatatcgtt ggcgtggatg ttgtacttcg ggtggcccga
     1861 catcggcgcc ttgatgccga ggttgagcag ccgccgggcg gacgcgttgg cgtgccacaa
     1921 ctggtacttg ctgtccttga aggcctcgcc gtactgctcg acagtggcgt cgaacccgag
     1981 ccggtccatc gtctcggcga gcgtcatgcc gacgtagaga tcgtcctgcc gcagcgcgtt
     2041 cgacacgcgc tcgggagacc aggcgatctc gccctcgaag atcctgccgt tcgactggaa
     2101 ctcggtcggc gcgccgtaag agacgccgat catcttgccg gcccagccgc cgcggatctt
     2161 gtcgtcgagc gccgtgcgcg cgatcgtgcg agtgggcgtg ggcttggccg gggcgaacgc
     2221 ggcgagcaag ccgaacacga gcactaccgt gatgtaccgc accggatcac ctccccggaa
     2281 atgaccgttc ctactttgcc gcattcgcgc ccctggtgcg cagtctgatc gcgtagagcc
     2341 cctcgctggc cgtgatgaac agtgtcttgt ggtcgcggcc gccgaagcag aggttcgcgg
     2401 tccagggctc gggtacgtcg atctgctcga tcttcttccc gctcgcatcg aagaccgaga
     2461 cgcccttgcc ggtcaggtac aagcgtccct cggtgtcgag ggtcatcccg tccgagccct
     2521 gctcgcagaa cagccgctgg ttcgccaggt tgccgtccgg ctgtatgtcg aacgcgtagg
     2581 tcttgttgcc gcggatgtcg gcgacgaaga gcgtcctgcc gtcgggcgtg ccgatgatgc
     2641 cgttcggctt cacgaggccg gtggtgacgc gccggagggt cttgcggtcg ggcgagaggt
     2701 agtacacctg ctcggtgccc tggggggggg cctcgtgcgt ccaccagtcg cgcttgtaga
     2761 acgggtctgt gaagtaaatg ccgccaccgg gactggccca gacgtcgttc gggccgttga
     2821 gcggcttgcc ctcgaactca cgcgccagca cggtgtgagt gccgtcgggc gcgatcgacc
     2881 acaactccgt cttctcgtcg gcacaggcga gcaggtggcc gcgcgcgtcg aagtacattc
     2941 cgttggctcg ccctgccggc tgcatgaagg tctcgagccg gccttcgacc gtccacttca
     3001 ggatccggtc gttcggctga tcggtgaaga agacgttgcc gttcgggtcg gcggtcggcc
     3061 cctcggtgaa ggcgaagccg tccgccagct tctccagctt tgcgccgggc gcgatgacgc
     3121 tcatgtcgtc tcccggtggc gccgccacct gcgacccgcc caaagagggc agggtgaaga
     3181 gaagtcccgc gaccgtcgcc agcagcacgc gatgcagccc gtccatggca tccacctcat
     3241 cccaccgggg gccacgcggt gattgttgcg cgggggcggc tcgttgtatt gtgcccttca
     3301 tgaagcttca gcgtcgcgag ttcctgcacc gggccgccct gggagccgga ggcctgctgg
     3361 ttggcagcgc cggcctttcc gccagtggcg ccggcctccg tgccgccgag actgccttcg
     3421 acccgtacgc gctcgtgccg ctgggcaaga ccggcttgcg cgtctcgcgg ttctgcctgg
     3481 gcaccgggat gcgcgggggc aaccgcgagt cgaaccacac gcggatgggc aaggccgcgc
     3541 tcgagcggct gattcgcgag agcttcgatc gcgggacccg gctgttcgac ctggccgact
     3601 tgtacggcac ccatccgtac gtcgtcccgg ccctcaaggg catcccacgc gaccgttacc
     3661 ggatcgtctc gaaggtctgg ttcatgcggg gcgggctccc cgagagcgag cggcccgggg
     3721 ccgacgtgac gatcgcccgg ttcctcaagg agctgggcac cgaccacatc gacctggtgc
     3781 tcttgcactg cgtcacctcc ccgaagtggc ccgacgacct gcgccgccag atggaccagc
     3841 tctccgagcg caagctcaag ggggacatcg gcgcgctcgg cgtctcgtgc cactcgctgg
     3901 cggccctcga ggccgcggcc gccgagccat gggtcgagtc ggtgcacgcg cgcatcaacc
     3961 cctacgggat gagcatggac ggcccccccg agaaggtcgt gcccgtgctc aggaagatcc
     4021 acgacgccgg caagggggtc gtcgggatga agatcgtcgg cgagggccgg ctgcgcgacg
     4081 atccggagcg gcgagccgag tcggtccgct acgccttgac cctcggctgc gtggacgtcc
     4141 tgaacgtggg ctgcgagagc attgccgaag tggacgacct ggcctcgatg gtccggcgcg
     4201 ccgggggcgc ctaggggtcg ccggggtcgg gagtaattag aagccggaga tccggcggga
     4261 cttctaatta ctcccgaccc caggcggggg tccacacccg gtattcgccg ctgcagtgga
     4321 gcagccccat caggtaccac aggccgtcgt agtaacggta ccgccccgtg gggatttcgg
     4381 cgttccagag cgcctcgacg aaccgccgcg cccgcgggtg ggtggcggcc aggcttgcga
     4441 ccgcgttggt cgcgacgagc gcggtcgagt ggttcgcctc gagctggacc gagccgtcga
     4501 gcgtccagcg gttcccgtac gagtcgattc ccttcgactc gaagaacgcc tggatccggt
     4561 cgctcaactc gcgctcgcgc gggtcggccg cccaccacga ccagtccacc gaccagttcg
     4621 cggccgtgcg ccaggcgtcg ggaccgaagg tgcccgaccg ttggttgaag cttgccgtga
     4681 ccggcgtgcc gtcgaagttc gcatactccg gcgccagccc ggttgccggg tgcgtcgcct
     4741 tctggaagaa gtcgcggctg acgcgcgccg cctccgccca gaacggccgg tcggcctcgg
     4801 ggccccaccg cgcccacagc tcgtaaaacg ccggcaggtg atacgaggga tcggtgtggt
     4861 ccggccgccg gttgtcgggc gtgaaccgca ccatcttgtg ttcgagattg aactgcgcgc
     4921 cgtcggtttc gacccggtcg cgccggctcg cccacggccc cgtgatgacg ccgcggttct
     4981 tgatgtcgga gagcagccgg tcggccatcg cgcggtaggc gtaaatcccg ctgccgtcgc
     5041 cccagcgcgc cgaggcgaag tacagcgcca tgacgtagta ctcctcgccg tctggcgccg
     5101 gcgactcgct gttcggcgta ccgtcggtct tcatcgacca tgagaagaag ccgtaggccg
     5161 ggtgcgcccg gtcgtcgtgg tacatgtagg tccgcgacca attccacagc gcgtcgaact
     5221 cggccttctt gtcgagctgg accgcgatca tcatcccgta cgacatcccc tcgctccgca
     5281 cgtcgcggtt gttgatgtcg ctcaggtaag ccagccggcc gttcgcgttt tcccccgacc
     5341 agtagaagac ggtttcgctg tccggattgc catggaacag ctgaccgaag gccttgtcga
     5401 tcttccgccg gatcgcctcc tccgagtatc ccgcctcccg gaacaggttc cggtagcggc
     5461 cggtcgcgaa cgccccctgc gttccctgct gctgcacgac tcggtcctcg gcctcgacct
     5521 ggatatccca cacgggcacg gccgggtgcg cgggcacgct cgaaccggag aacagcagta
     5581 ccagcgggag caggctcgtc aagggcaaca taggatctcc tcacaggcgg gacgcccgtg
     5641 ccactcgggt gtcctttcgg gcaggggctg gcggggtggc ccgaaccgcg ggccgccggc
     5701 attatatgcc ggggcggcag catccggggc gggcactgga ggcaggcaag gagcagggcc
     5761 gaagccctgc tccaagatgg gtcagtcagc ggcgcgtgaa caactttccg accatgccca
     5821 gcacatcgtc cacgaccgaa ccgtcgcggt tcgcgtcgag catcggggcg agcatgttca
     5881 acaggtcgcc gccggcgccg gcgcccccgg gcatcgaggg ctgcgaggcg cgcttgctca
     5941 tcgagcccat catcatcgcg gccacgaccg gcagcatctt cttgaggatg ccctcgccga
     6001 tcccggtctg tgccgaggcg cgcgcggcca cctgccggct cacctccttg ctgccgaaca
     6061 cgtgcccgag gatcccgttg ccgtcggtga tcgtctcggg gctggccagc gcgccgggat
     6121 cgtccaggta gcgctggtgg cgcccgccgc cgagcgcgcc cagcaggctc tcgaggccgc
     6181 cctcgctggc catgttgcgc tggaagccgc tcgccagcgc gggcaccagg gccgagagtg
     6241 ccgactgcac ctggtcttcg cccaggccga actgctcgcc gatctggcgc gtcgcaccgc
     6301 cgccctgcgc gctgagaatc gcttcgagaa tgttcatgtc atctccgggc tgaaggttgg
     6361 gataccgcgt ctccattcta gcaggccacg gcgaacgggc cccgacattc agcggcacct
     6421 ctgcctcgga cgtacaatgg tttcgccccc ggagaccgag catgaagcgc tcttcatcgt
     6481 ggctcgccct cgccgtcgcc ctcgcgctgt tgcccgcggc cgccgccgcc caggatccgc
     6541 cgaaggagcc gccgcccccg ccgctgttcg agggcaccgt ggatttctcc ttcgtgtcga
     6601 ccaccggcaa cagttccacg cagtcgatcg gagtctcggg agacaccacg taccggccgg
     6661 acggctggga gatccgcaac aaggcggcct tcgtacgggc gaagaccgac gaggagctga
     6721 gcgcgcagtc gttggtgtac ctgttccggg ccgccaggaa gatcaccccg cgcctgtcgg
     6781 ggtacgggca gttcgactac acgcgcgacc gcttcgccgg catcgagcac cggaacgccg
     6841 tgctgggcgg cctggagtac ctcctgtaca acacggcgcc tcacgacctc aagatcctcg
     6901 gcggcgccgg gtacgtgaac gagcagcggc tcagcgggga cgacgtgtcg accggggcgc
     6961 tggacgcggg ctgggcctac aagctgaagg tgtcggagac ggcggagttc accgacgact
     7021 tccggtgggg cctctccttc tacgagtcga gcgactggcg gatcgggcac gtcgcggcgc
     7081 tcaccacccg ggtgcacagc atcctgtcgc tcaaggtgtc gcacaccacg cgcttcgtca
     7141 actttccgcc gccgggcttc gagaagaccg acacgatcac cgcggtcgcg ctggtggcga
     7201 agttctgagc ctcgacgcct ggcctactgg cggggcgtgt gcgcctcgat ctgtccgggc
     7261 ggcggcgggt cgtcttcctc cgccggcgcg tccgccgcag agccccgcga ttcgagcagg
     7321 cggtcggcgg ggaggcgcag gcggctgcgt tccaccgagt cgacgccggt ggcggtgatg
     7381 gcgagctgcc cgctctcggt ccgctgcacc cagcccttct cgcgaaggta ccacgtgtgg
     7441 aacgcgacga gctcgacggg gattcgcagc agccgcgcga gctcgtactc gccgaggccc
     7501 ggcgacatca tgtcgcgccg ccgctgcacg tagaacagcg agagcagctt ctcgcgcgtc
     7561 gcctggtcct cggcgaacgc gctgccgtcg ctggcctccg aggccaggcg ccacttgcgg
     7621 ttccagtact cctggtagcg ggcgtcgtac gtcgcgcgcg ccgcagggtt ggtcagcgtc
     7681 gcgtgcgcca ccaccagcag gcggaagcgc tcggcgttgc cgtcacccgc gtcggggtgg
     7741 cacttcttcg ccaggtggcg gaagacgcgg tggatcaggt cgtcgtccgc gttcgggctc
     7801 agctgcagca gctcgtaata gtcgacgaac tcctcgctca cacgcgcctc cgggccacat
     7861 tctacatgtc ggccgcgcgg gtcagtacga ataggtgagg gtcgcgctcc cgtagcggca
     7921 cggccgccac tgcgccgcgt gctcgcgccc ccacgcgacg aacgagacag ttgcctcgaa
     7981 ccggctgttc cggatcgaga ccccgacctc gccggccggc accgcgtgcg ccttctcctg
     8041 cacgcgcggc ccgtcgctga aggtgttgcc gtcgagcgtg aggttgcggg cgacgaagaa
     8101 gacgcccgcg cccgcgaaca cgtaagcgcc cgtgccgttc ggcgatcccg tagccgcgtg
     8161 ccgtgcgggc agtggcacgt tgcccaggcc ccgcgccggc gagctgccga agtcgtccgg
     8221 gaggtgacgg ccgaaccgga cgtaggcttc ggcctgggcc tggacgagca ggttgcccgc
     8281 gctggcgccc gccctcggga tcgcctgcag ggtccacccg cccggcgtgg cgtgcaccgt
     8341 gaagcgccgg cggtgctcgt agaacacgtt gagcagcagt tcgttgcgga gctggtaatc
     8401 ccatccccgc gcctggtcgc tcagcgtgac ggtgtggatg gcccgctgga ttggctcggc
     8461 aagcgaggcg ggccccacga cgccggcgag gagcttcagc ccgtccaggc ggtcgcccga
     8521 aagggcctgg gtggccaggg ccccgtagag catgccggcg taggggcggt cgctcgggtc
     8581 ggggacgggc gccaggatgt tggccggggt cgagatgacg tgccccagct cgtagctcga
     8641 gacgaggcgg gagggagagc cgccgaacac gctccagatg ccgcccagcg gcccccgccc
     8701 gtccagtgac agggccagcg agaagccgct gctgtagtcc ttgtcggtgc ctgccacgat
     8761 gtcgttgtcg aggcgcaacg agacgatgtg ccgcgggcgc gcaccgacgg cctccctggg
     8821 ggccgcaacc gcgcggccgg ccggcggatt cggcgcgaag tcccgggccg gcggcggtcc
     8881 ggggacgccg ttggcgagcg cgagcgcgag caccagtgcg gccgcgggca tccttcgact
     8941 atatactgtg cgccccggcg atcatgaccc gacgcccacc ccttcagacg acaatccgtt
     9001 ccctccgcgt ggcggcggct gtgctcgccg cgctgtgcct cttcgcgccg ggcctcgccc
     9061 tgggccagga gggcccggcg ccgtccggaa gcgaagaggc agccgccttg cagggcgcgt
     9121 ttccggatgc aacattcgac gcgtacttcc ggcagtacct tcctctgaag aacgtgtact
     9181 cgccttacta cgcctgggac gcggccatgg ccctcgacct caccgtgttc aggaaggggc
     9241 agggcgccgt ggatttcggg gcggtggtcc agacggcggg caccgagaac ctcggctcgc
     9301 aggttggcgt gggcggcacc ggctacatcc tgcgcgccgg ctacctccgg tcgctctcca
     9361 gggccgtgac gctgtcggcg ggcatcgcgc acctcagctc gcacctgacg cgcgacctcg
     9421 acgacaagac cgacgagcag cggcgcaagg ggatgctcat cccggacgtg caggacccag
     9481 acgagtacaa cgtcgtctac ctcaaggcgc acctggcctt cgagcgctgg ccgctcgagc
     9541 ccgagttgga cgtcatcctc gagccggtga acttcaggtt cgacttcggc tacgccccct
     9601 acgcgcggcc cctctacctc gggacgcggt tgatcctctg gcgcggggtg cgcaagaccc
     9661 tggcgctcga gacgcagcac gaggtcggca acaacggctt caacaacttc tgcctgctgc
     9721 tcgaactgtt tccaaaggcg cagcacggcg ggcgcttcca cctgttcgtg ggcgggtcgc
     9781 ccggccgcac catgcacgtc agcccgaacg tgtcggccct ccgggacggg gtgaccgcag
     9841 gaatccggct gcgcttccgc acttgatccc cccggtctgg gtgcgccgcg gcgcggcctt
     9901 gttcggtcct gctatactcg gctccttcca tcgaaggagc atcagatgga tccgcgttac
     9961 cccatcggcc ggttcgaccc cgaggatgcc acaccgctgc cggaggtgat cgatcgcatc
    10021 gcccggttgc ccggccagct tgccgatgcc gcacgcgacc tcgagcggcg ggggaacctc
    10081 gatgtgccgt accgcgaggg gggctggacg gcgcgccagg tggtgcatca cctggccgac
    10141 agccacctca actcgtacac ccgcttccgc ctcgcgctca ccgaggagcg tcccatgatc
    10201 aagccgtacg acgagaagcg ctgggccgag ctgccggacg cgaagtccgg ccccatcgag
    10261 ccgtcgctgc acctgatcga ggggctccac gcgcgatggg tggacctgtt gcgggggatg
    10321 agcgcggccg atttcgggag ggtgttcgtc caccccgagc gcggcgaaat gtcgctcgag
    10381 cggacggcgc ggctgtacgc ctggcactgc cgccaccacc tcgcccacct ggcgctgatc
    10441 aagtgaggta aggggctgcc cacaggcgag acgcctgtgc cactacccac agcggccgtc
    10501 gctactaccc actgcggcct gtgccactac tcgcccctac cggccgacca gcgcggcgct
    10561 gccccagtag acggccgcgg cgatcagcgc cgaggccggg atcgtcagga tccacgccca
    10621 gacgatccgc gtcgcgaccc cccagcgcac ggccgagagg cgcttgatcg agccgacgcc
    10681 gatgatggcg ccggtgatcg tgtgcgtcgt gctgaccggc acgcccaggt gggtggcgcc
    10741 gaagagcgag atggcgccgg ccgtctcggc ggcgaaccct cccactggct gcagcttggt
    10801 gatcttcgac cccatcgtgt ggatgatgcg ccacccgccc gacagcgtgc cgagcgcgat
    10861 ggccgcgtgc gccatgagga cgacccagat ggggatcggc agctggccgt tttccagctg
    10921 caggaagccg ccggccacca gggcccccga gatgatcccc atcgtcttct gggcgtcgtt
    10981 gcccccgtgc ccgaggctgt aggccgccgc cgacgccagc tgcagccgcc ggaaccagtg
    11041 atcgacctgg cccggcgacg tcccccggaa cagccatagg atggccacca tgatgaggaa
    11101 gccgagcacg agcccgatga gcggagcgag cacgatgaag atcagcgtca gcgtccagcc
    11161 gccgggcagg atcgcctcaa acccggcgcg cgcgaccgcg gcgcccgcgt atccgccaat
    11221 cagcgcgtgg gacgagctgg ttggcaggcc gtagtaccac gtgagcaggt cccaggtgat
    11281 ggcgcccatc aggccggcga agatcaccac gaagttcacg acctggaggt ccaccaggcc
    11341 gccgccgacc gtcttggcca ccgcggtgcc gaaggtgaac gccgcgatga agttgaagaa
    11401 ggcggcccag acgacggcct tgccgggcgt gagcacgcgg gtggaaacga ccgtcgcgat
    11461 cgagttggcg gcgtcgtgga acccgttgat gaagtcgaac gccagcgcga cgaagacgag
    11521 caggaggacg gcgaggatgt tcgtgtccat tcggatcgcg gcggtcagtc tgcgtgagtc
    11581 ctgagtgtac actgcacccc ggccgttcat ccacacgcgt atttgccacg aaggagggtc
    11641 catgccgccc gcgctgatct tcgtcgtcgc cctcgtctcg atcatggccg ccctgctggt
    11701 tgtcgtctgg cggaaggagc gcgcacgaac cgaggcactg cggcaggtgg ccgcgacggc
    11761 cggcctcatg ttccagccca aaggggatct tgcggccctc cgggcgctgg gagacgtgcc
    11821 gctgttcgag cggggccatt cgaggcgggt gaggaacctg atgacgggcc gagtcggcga
    11881 cgatcacctc gccgtcttcg actaccgcta cagcacggga tcgggcaaga accagcgcac
    11941 ccgcagccag acggtcgtgc tcctgccctc ggcgacgcga tcgctgcccg acctgcagat
    12001 ggcgcccgag aaccccgtga ccaggctggc cgagaagttc ggcttccagg acatcgacat
    12061 cgactcgagc cctgagttct cgcgccgcta cgtcgtgcgc gggaaggacg agcaggcaat
    12121 ccgcgccgcg ctcgccgccc gcgccacctc gtacttcgcc gcccacgagg ggtggacggt
    12181 ggaggtgcgg tcgggcaccc tggcgatcta ccgcgaggat cgccgtccga agccccagga
    12241 cctgcgggcc ttcatcgagg aggcctgcac ggcggcgagg aacctctgac cggtggggca
    12301 ggccgccggg cctgccccgc cgatgaccgt ccacgggcga gaagagagga gccgccccgg
    12361 cctcactact gcaaggcgag ctggccccgc acggcacccc ccgggttgat cgtggtgtgc
    12421 acgttgaagt agaagccgga ggggcccgag accagggcgt tggccagcga ggcggtcacg
    12481 ttcacgttct ccttggtgaa cgaccccgac ccgcttccga gcgtcacctc gccgttgccg
    12541 aggccggtgt tcaccgcgat cccggcgttc tggccggccg gggcctggtg gatgtgcgcc
    12601 ccggtcagca ccgtcccggc ggggaacccc gagaggttga cggtgaagtc ggcgctgccg
    12661 cccgtgatgc tgcccgccgc gtcgcgcgtc acgtcgagcc tcaccgtcac cgtgccgctg
    12721 gcgcttgcat ccgcgttggt gaccggcgga acctcgttgc ccggcagcag cgtcgaggtg
    12781 aaggtgaacg tgttgtttgg cggcgacggg ctgagcgggt tgtcgttgtc gtcgtcacag
    12841 gccccgctcg ccacgaccgc cacgaccgcc aggagcaccg ccgcgagtgt ccgatacctc
    12901 attgcctcac tcctcgtttg tgaagggtcc atcagtagaa cttccggccg aggttgaatc
    12961 ccatgtagag ggtgtcgggg aaccctccct gggacatctg cccgaacgtg gtgccgaccg
    13021 agttggtgaa ggtgagcagg aacatgtggc cgccggcccg gcgctcgatc ccgaacgcgt
    13081 agccgggcga tcccggggcg tagccgccga tgcgcggcga cacctcggcg acgacgaaga
    13141 cgttgcgcga gagccgggcc cggccaccga ggccgaggaa cccggtgttc cggtcgccct
    13201 ccgggagccg cgcgctgtcg tgcacccaca tcggcacggc gtagagggcg agccgatcgc
    13261 tgaaagtacg cgagaccacc gcgccgaggg cgggcgagcg gtgggcgccc ccgccgactc
    13321 cgtggtcgtg gccgccgtcg ccgcccgtgt cgccgcggcc cttgaagttg ttcgtgccct
    13381 cgatcgacgc gatgggcgag atcgagacgg gccaggcgcg gccctgccgg atcgcgtcgt
    13441 actgcccgga gaactgcagc gtcttgtcct ggctgctccg gtgcacgacc acctggaggt
    13501 tgctgagcgg cgcgaagcgc agctcgaggg cgatcacggc gccgttgtcg aggccgaaca
    13561 ggtcctcgag gtggtcgctg aagctgccct ggcgcaggtt cgccaggaag cggtgcgtca
    13621 ggcggaagtt gaacccgaag cgcggcagct gcagcgtggt gggcagcgtg acgaccgaga
    13681 aatcggcctc ggtcaggctc gagtcgactt catcctcttc gtcctggtgc gcccgttcat
    13741 ccgggggtgg cgcggcgggc gacccgagcg gtgcgacctt cgcggcggag ggggcatcgg
    13801 cttgcgcgag ggccggcgca accgctccgg gcgagaggca ggtcgcgcag gccagcaggg
    13861 cgacagcact catgctcgtc gtcatgcccc ttgctggctg caaggaggag accgcaagat
    13921 tccctgcgat tatggccgtg tcggggggga tcgcgctttc gcgcggcgta agaggttacc
    13981 ggcggcgaaa ccctcctgct accggttcct tggcgagcgc gacaggtaac gcgcaatctc
    14041 ggcggcccga agcgcatcca cgtatgagtt gaagcggacc cgcagcaggt cgagcggctg
    14101 caccgtgagg aagatgtcgc gctcctggtc gcgcacctcg gtaacctgcc gccattccgc
    14161 ctcgagcttc gaagcgccaa ggtacaggat gcgcctgtcg gtgagaaaga gcttcccgcc
    14221 ctggtccatc aggccgtcgg gcgaggaggc gggcgcgagg aaatgacagc gctcgcccgt
    14281 gatcgccggg tgccgcgtgg gaatcacttc gagcggttcg ccgcgggcaa gccgttcgcg
    14341 caggtcggcg gcggccagca ggcccgccag gtgctcgcac tcgagcgccg cgtcctcggc
    14401 gtcgagcccc agttgcttcg gccgatcgag aagcgtctcg atccgtgacc ggtcgcccga
    14461 tgcggccgcg gcttggatct ccgcacgaaa cgtcttcagg tgcttctccc gcgccaggtc
    14521 gccgggcgcg accatctggc ggacgaacga cggtagcttc accaggcacc cccggaatcc
    14581 ggaccggcgg cagccggccc tgcgccccct accctgtaag agacggttcc cgccgtggtt
    14641 gcgttcgccc ggcgtgggga gggtgaggcg caagtcgcga ggctcaccgg cccgccgggt
    14701 cgagccttgg cggccgcacg ccgtgccggg ggctacctgc cagggggatg tagtccagtt
    14761 cgcggcacgc gcggacgctg cgaagcagca ggccgccgcg gctcatgtcg ccgcgcccca
    14821 cgaactcgcg cgggaacacg aggcgcgggc cgagcacctc gaccagcacc gccgcgtact
    14881 tctcgctcgc aatgctgccg agcagcacca cctggtcgcc gtccccgagg aacgcggcga
    14941 gcctgcgggc gccttcttcc agggcgaaaa cataggcacg gttcgcgtgg tgcacctcca
    15001 ctgccgcgaa cgcggcaatc gccgcggcgt ctaccgccgt gtcccaggcg agcacgcctc
    15061 gcgacggcgt gatcacgaag acgccgtccg ccagggccga tcctgactcg tccgacggca
    15121 ctcgcgcgaa cgcccgcgcg taagcgagct tcccccggaa gtacagctgg ctgatgaagg
    15181 cgaacacctc gccgatgggc gcaccgcctg cggaccggag ccggaccgcg agggcgaagg
    15241 cagccgacgg cgacagcagc tgctgcgcgc gcctgccgct gcagctggcc ggcgagagga
    15301 ggaagagacg cgatggcacc gtgaaccagc cgcggcggac ggcccggagc cgcccgccgc
    15361 cgtacggttc gacctgcaag ggccgtgcgc gcccggcgcc cccggcccca catccgctcc
    15421 aagacgatct cgatcagctc gagattcgtt ccctgcctac agccggttgg atcctcctca
    15481 tgtcggaagc cgttcgagcc gacacaagag atatgttcgg gcgatctcgg gcggcgccgc
    15541 ggccctggcg cagcatcgtg gcgaaggctc gccgagaggc aggggccatc tggcgtcgtg
    15601 acgggtggct gatcgttgga ctcgttctcg ccgtgctggt cggcttcgag gggtcggtcc
    15661 gcaaggcgtt ccagttcgcg cggcaggtgg agctctcgta cgggctcgcc ctgctgcccg
    15721 cccttttcat tctctgtgcc ctgctggcgt ttcaccagta caccaaacgc caggaactga
    15781 aacgacaggc ggcggctgcg gcggcagagg ccgcccaggt caagcagcgg ctgctcgagc
    15841 tcgagcgcct cgccgccttc ggccacgccc tggccggatg cctggggttc gacacgctcc
    15901 agcaggtcgt ctggagacac ctgccggggt tcatcggccg ctccgaggcc tggctgctca
    15961 cctacaacga aggaacctgg cgcgtgctgg ctgacacgac cggcctggag tcggaccgtt
    16021 cgagccagct ccaggccatc gcggtggagg cgctgtcgcg cgggagcgag gccaccgggt
    16081 gcgcggacgg catcgagtgc gccggcgccg tctgcttccc gatgatcgtg ggcggccagg
    16141 tggtgggcgt gctcggcctg gacgacgccc caaccgcgac tcccggctgc cgccgggcga
    16201 tgtcggcggt ggcgacgatg ctcggcatcg cggccaggaa cgtgcagctg ttccgcgacg
    16261 tccgcgacga cgccctccac gaccggctca ccgggtgcct cgggcgcgcc cacggcctcg
    16321 agacgctctc ccgcgaactg gtgcgggtcg cgcgcatgcg gcgccccgtg tcgatcgtga
    16381 tgttcgacat cgactacttc aaggccatca acgaccggta cggccacctc gtgggcgacg
    16441 aggtgctgtc ggcggtgggc gaacgcctcc gcagcctgct gcgcggcagt gacacgcggt
    16501 gccgctacgg aggcgacgag ttcctggtga tcctgcccga gacgcccgcc gagggggccg
    16561 tccacgtggc cgagtggctg cggatgcagc tggcggccgt tcgggtgccc tccctggcgg
    16621 acgcctcgac cccgcttcgg gtgagcctgg gcgttgcgac caccgacggc cggctcgaag
    16681 tcgaggagat gatcgcccgc gccgacgcgg ccctctacga ggccaagagg tgcggccgca
    16741 actgcgtgcg ctcgtacaag gcctcggaag agcccttcga gctgggcgaa gctgtgtaat
    16801 ccccccgccc gcctgttctg tccttggcgc tcatttgcgt aggcatggtt gtgcgcggct
    16861 gcagggcgaa gagccttcgc tcgcgcacgc ctgtctccgg cgaaccgttg cgccgcttca
    16921 gccgtcaaag gtggtgtgcc gctgacgcgt cttctctatg tcgcccttct ttcgtgcctg
    16981 ctcggcctgg ccgcggcatc cgcctcggcc ggtctccctt ccacgggaac accatcgcct
    17041 ggaacgggca gggcggccgc accgtcaggg gcgaggatcg aggccctcct gctgcccgag
    17101 ggaaccgccg gcgcggtaaa gctggatggc gagttcaccg agggcgcctg ggaaaaggcg
    17161 ccgcccacgc gcggcttcct gcagcgcgac ccgaaagagg gcgccgagcc ggcgttcgac
    17221 acggaaatcc gcgtgttgta cgaccgcgac ttcctctacg tcggcgtccg cgcccacgac
    17281 tcggcccccg gtgcgatcgt cggcatcagg acgcgccgcg acgaggagtc gccctccgac
    17341 tggctgcgcg tgatgatcga ctcctaccgc gaccgccgca ccgcgtacga gttcgcggtc
    17401 aacgccgcgg gggtgaagca ggaccggtac tggttcgacg acgggaacag cgacgtctcc
    17461 tgggacgcgg tatgggatgt ctcggtgcgg cgcgacgagc agggctggag ggccgagttc
    17521 cggatcccct tctcgcagct gcgcttcgcg ccgggcaagg gcgacgcctt cgggttcgcc
    17581 gtggtccggc agatcgggcg cctcaacgag acc
//
LOCUS       DAOPQX010000034        17609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3843_length_17609_cov_4.621967, whole genome shotgun sequence.
ACCESSION   DAOPQX010000034 DAOPQX010000000
VERSION     DAOPQX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17609
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3843_length_17609_cov_4.621967"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..2352
                     /locus_tag="PKK95_03300"
     CDS             250..2352
                     /locus_tag="PKK95_03300"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC17265.1"
                     /translation="MRKSMLWLACAGAVVAGGAAVGAQMAPWLQWTLLPRAAWNEIAG
                     EASGENAWKMVMETGGYDRDRSAAEYAGTFREAQFYLDKAREYGLEDAEIVRFPGGRT
                     WDAVKGELWEVTPKRQKLASYLDSAAMLATGSANTDVKAELVWVGAGRPSDIEGKDLA
                     GKIVVTEGSIPAVYRLACVDRPAAGVVGVMNSPRPLFDPLQMAWASIVGRGPAQESEA
                     VAKFAFQLPPREGDLLKRRLLAGEKISVHAQVVAEHRDYTIQDVVWSIPGTDPSKGEV
                     ILSAHLFEGYVKMGGNDDISGCAALLEIARTLNTLIAEGRLPRPARTIRFLIGPEFSG
                     TGPWVKANRALMEKTLCNINYDMVGLRLSRSQSFMCLMRTTYGNPHYINDVMENYYRF
                     VGEGSRERIPTNAPSQRLVAPTGSDEPFYYNIEVHYGASDHEVFNDWGVQVPGVMVID
                     WPDRWYHTSEDHVDKTDPTTLKRVVAIGAGAAYTIASADDEMAIRIAGEVASNATARL
                     RHQGARAIEELNGATAETLPDAYRAARGYVEAALINEKETLETVNELAGDAKRVALHL
                     AALQHGVEQAASAQLSLVDAQMRAAAARLKVAPVALKPGELEVKASKVVPRPTALVTA
                     DGYQGWREALGKVPQEQRAKFPIGRLDTGELQRLVNGRRSALDIKKMLDAQAETKADL
                     QQVLNYLEVLALAGLVEK"
     gene            complement(2310..3251)
                     /locus_tag="PKK95_03305"
     CDS             complement(2310..3251)
                     /locus_tag="PKK95_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17266.1"
                     /translation="MRSAGASLERTDERTSTEGRGQGQSVVVFSRLDPAVLDDSAHAR
                     VTLREGLGRLSARGIPVVVASRATRAEVELLRDELGLTDPFIAENGAAVFVPCGYFPF
                     PLPASGTRSGYHTFERSIAHDAVVRALRRAANQARVSVVGFSDMSISEVARETGLTLL
                     QARLAKLREYDEPFRVLDSDAAARARLFKTLRGAGLQVWSEGRFDHAVGSADMGACVG
                     LLRGLYARGRPVLTVGVGAADADLDLLKRVDVPVLVPAGSRRGPVAVTRSVPRARIAR
                     SASPEAWLQTLEEAVREAPDDRSAYFSTSPASASTSR"
     gene            3364..4809
                     /locus_tag="PKK95_03310"
     CDS             3364..4809
                     /locus_tag="PKK95_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC17267.1"
                     /translation="MDTTAREEVRRARRQQASQAVGGPSIGRLLLGGFVLIFALWLVS
                     GFDLVRRLNDVEERQAALTTAFRRAERSLNAIQAQVLLGSVYLRDALLDLDRDSADDY
                     HQRLEQSRADIEQALAQYRPVVQSETERASFRTLQAEVDDYWNSLLEPVLTWDATRKA
                     VEARALLRRQVIPKRELIARISARIQELNREAFELQQAEVSQIFRQLHTRVWTTSGLT
                     LLMSLVVAGAVTRYATILEDRIRQQMVRDLEHARDLQRLSAKLVDVQEQERRSIAREL
                     HDEVGQALTAIKVELAVAERTGGLSEKVQLALQDVRAITDRTLQQVRDLSQLLHPSLL
                     DDLGLPAALEWYLKGFSRRTDVKAELLQDRMEQRLPPQVETCVFRVVQEALTNVARHA
                     SASSVRVYLQRLPHTVLLTVEDDGVGLAQEDEAEPKRGLGLLGIRERAGELGGTFRIE
                     SQPGKGTRLTVELPVEASQDEFSHADAHPAG"
     gene            4784..5434
                     /locus_tag="PKK95_03315"
     CDS             4784..5434
                     /locus_tag="PKK95_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC17268.1"
                     /translation="MPMRILLADDHTIVRHGLRKILEESRDWQVVGEAGNGRDAVRLA
                     LELQPEVVILDIGMPLLNGIEAARQIARRLPDTKILILSMHADEAYIIQALEAGAKGY
                     LLKDTADADLLGAVTAVIRGKSFFSPAVARVMLDDYVRRLRDRGIVDRYDTLSERERE
                     IFQLIAEGKTNKEIAEILGVSPSTIETHRGHIMEKLDVHSAVEMVLYAVRKGIIST"
     gene            5442..7736
                     /locus_tag="PKK95_03320"
     CDS             5442..7736
                     /locus_tag="PKK95_03320"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOC17269.1"
                     /translation="MIHPHHLVIAAELSVQPRQVAATAALLGEGATIPFIARYRKEAT
                     GNLDEVAITRVRDRLAQLGELDERRAAILASLEERQLLTDPLKAAVEAAATMAALEDV
                     YAPYRPKRRTRATIARDAGLEPLAAVIFERQEETDPQAAAAAFVDPAKKVPNVDAALA
                     GARDIIAEQVAETAAVRERMRRLFWQSGTVHSTVVPGKETEGVKFKDYYDWSEPVASI
                     PSHRMLAIRRGEAEGVLRVRVAPPEGEALSAVESLTVRAPGRPAAEQVRLAAHDSYKR
                     LLGPAIEGEIRFDSKKKADFEAIRVFADNLRQLLLAPPLGQKRVLAIDPGFRTGCKVA
                     CLDAQGKLLHHDVVYPTAASAAQARHAGDTLRALVERFRIEAIAIGNGTAGRETEAFV
                     RGLGLPVVVAMVNESGASVYSASEAAREEFPSHDVTVRGAVSIGRRLMDPLAELVKID
                     PKSIGVGQYQHDVDPLAIKRGLDDVVESCVNGVGVELNTASRQLLSYVSGVGPSLAAK
                     IVSHRDERGPFPSRRALLEVPRLGPKAFEQCAGFLRIRGGEHPLDASAVHPESYGVVD
                     AMARDLGCSVADLMRDAGLRQKIRLERYVNERVGLPTLRDILDELARPGRDPREEFEA
                     WRFAEGVEKIEDLEPGMVLPGIVTNVTAFGAFVDIGVHQDGLVHVSQLADRFVKDPSE
                     VVKVQQQVRVTVLEVDLPRRRIALSMRTAPAAERRRDDRRSAAPRPAASLRPNSPGEL
                     RRPAERARPGGFNNAFADAFRRKG"
     gene            7742..8599
                     /locus_tag="PKK95_03325"
     CDS             7742..8599
                     /locus_tag="PKK95_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOC17270.1"
                     /translation="MVRQAWRRLAIVLLGLLLSAGGGDAKAGRVLRLATTTSTEDTGL
                     LHAILPSFEQRCGCRVDVVAVGTGQAIALGRRGDADVLLVHARALEEQFVAEGHGGPR
                     QPVMYNDFVIVGPAGDPAHAARARTAAEAFRRIAARSAAFASRGDKSGTHVAEQAIWQ
                     AAGVCHEGSWYRSLGQGMGETLTLANEVPAYTLTDRGTWLSMRGRLDGLRLLVGGKNP
                     AENEDPSLRNEYSVIVVSARRHPGVAEGLAQQFAGWLRSPGTQQAIGRFGVERYGQAL
                     FHPDAGRQR"
     gene            8596..9258
                     /locus_tag="PKK95_03330"
     CDS             8596..9258
                     /locus_tag="PKK95_03330"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17271.1"
                     /translation="MTDQFASPELLRIVALTLEVSGAALLIATLAGVPVGALVGLLHF
                     PGRRLVTLALYTGMGLPPVVVGLFVYLLLSRSGPAGGLGWLFTPAAMVAAQAIIAFPL
                     VAGLTMSAVSGVERTVYQQVVALGASSWQTGWTVLREARIGVTAAIVAAFGAIISEVG
                     AVMMVGGNIEGQTRVLTTAIVLHTRQGEFAMASALGLTLISLAFVANALLLRLQGGVR
                     DR"
     gene            9258..9983
                     /locus_tag="PKK95_03335"
     CDS             9258..9983
                     /locus_tag="PKK95_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC17272.1"
                     /translation="MARDVVYRLESVRQEYDGRVVLDIDRLDIARGETLAVIGPSGSG
                     KSTLLRLLQFLEAPSEGRVSFEGREMNGRVALATRRRVSTVFQRPIALTRSVEANVAY
                     GIELRGRRPDAGEIRGLLEALDLAPLARRPAASLSGGEIQRLAVARALATRPDVLLLD
                     EPTANLDPRNVRLVETLVQERRALGMTIVLATHQIFQARRLADRIALLLDGRIAEAGP
                     AQALLDRPADARTRAFLSGEMIY"
     gene            9983..11623
                     /locus_tag="PKK95_03340"
     CDS             9983..11623
                     /locus_tag="PKK95_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC17273.1"
                     /translation="MPVRTRRLRVIVSAAAVLVLVPLPLLAQARGLRAIEVEDMQRWL
                     RFLGSKEFRGRAAPSIELDLASRYLAVEAAHAGLEPLLPGGSFLQHVPVEVTTISGER
                     SRIRLSSPGGELRLAFPAAFTSSVQLPGDWSASGPLVFAGTALAGPQPKWDDAVASLR
                     GRWVVVFEEDGRARTADERRAASAARDQLLRDRGALGLIVIIAPDREARLAAQGLDFD
                     VSRRLRFLDVDTVNPPPPPLPGAAPPPVEPMPVNAVEVRHQAAADILGVTPEEVARMA
                     HDASENRVVPPRPLPGRSADVEIFFETRQATTPNVVAWLPGSDPKLRGEYVLIGSHHD
                     HNPPREGRVFPGADDNGSGAVGMLSLARALVAERPKRSVVFVWHTAEERGLVGAYYFV
                     QHSPVPVEKISAVLNLDMISRNDPNAIYLIGSNQVSSELDASLRRMNDRHVGLSLDYK
                     YEDPGEPSRFFFRSDHYPYIRYGIPAVWLFCGTTEDYHREGDMEEKVDYAKMEKVVRL
                     AYLVAMDVGNKAGLLQLDVHPQIKARGAHNMKVVWRRR"
     gene            complement(11626..12945)
                     /locus_tag="PKK95_03345"
     CDS             complement(11626..12945)
                     /locus_tag="PKK95_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17274.1"
                     /translation="MNAGLRLRVAILSAGVLAVATGASAQGTPAWGRVSFFSSAVSTT
                     PDAGSSSSFTELIGTFTVESRNAENAAFEYRADLRMAGYPGSGGRVRRVSVYDAYVGA
                     RLMEGRIGIRAGQMWLNDLGGLGSVGGGLVELRQKARTGRGRWRAGVFGGLEPKILEA
                     GYERDVTKAGGLVAFDGTGARRHVLGYVYLRDRGRTERSVLVATNFLPVGPKLFVYQA
                     AQYEMGGPGVSGSGTLGYLFGTARYTASSFLELQGSYHRGRSIDARTLLRDQLDGRPV
                     LPRALEGLRFESASGRLTVTLARGIRVYGGYGRDRNNRQDRSTDRITYGFFVSDLAGT
                     GIDLSASDSRMEGGNGRSFNSWYVSVGRSLGSSIYLTGDYASSLSVLRFVSADGFAIE
                     TRPRTRRASLSSTIRLPRRMSLVATLERVLDDDATQTRLLSGISYRF"
     gene            complement(12961..16146)
                     /locus_tag="PKK95_03350"
     CDS             complement(12961..16146)
                     /locus_tag="PKK95_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17275.1"
                     /translation="MILLAVLAGPPVAHAQIAALVSPGPLSRAHASLEGIANCQKCHE
                     PGRQVTASKCLSCHKPIAGRIAARKGVHRDAGDRCVDCHAEHAGLDAELRPFDQGNFD
                     HARETGFPLDGLHAPLARRCASCHKTRSFLTLRPECGSCHADPHKGSLGPTCTTCHST
                     AVRFAVARNNFDHARTRFPLAGAHRKVDCARCHTNRTFTGVKFASCTDCHRSPHRAPL
                     GSTCTACHDNESWRTRKMDHARTSFPLAGRHQTVPCASCHKQPAVRVALRADRCANCH
                     VDVHKGQFKQDCGACHKENGFAAAPFDHAKETRFPLTGRHASLACGQCHKSASTLSPS
                     RAGRTGRPVLFGGLSSTCASCHQDVHKGEAGTACQTCHGAEAFKPVTRYEHGAALAAF
                     FRGAHQGASCAACHGGQAHPHVWRVAGRPTGSSSAPPGAIGSWRFKGFSTACAGCHVD
                     AHRGGLGAACETCHQVEDAGFKASAFSHAKTAFPLTGRHQAAACAACHKPADAGGRVN
                     YRGTATSCAACHKDVHLAQFGERCESCHATDSFKIPVYTHRWAGFERFFVGTHATLNC
                     RGCHPQGEAAFPGGRGTAVRYRGTGTACASCHGDEDVHHGALGPSCETCHEPARWATA
                     SRAFHKSGLFPLEGRHLTVPCASCHVSGMTKGTPTQCFDCHWARRQDDRYHLRLGSQC
                     EQCHRPTSWTAVTWNHAARTGYALNVAHAVLRCDNCHRNGTFINTRLDCFGCHEEDYS
                     RTRNPNHGEAGFPTDCEACHRQTASTWEGGGTFNHQSIFPLVGVHATQACAACHAGGL
                     YRGTPRECVGCHQDDYQRTRDPNHAAAGFPTSCEACHRATDASWRGVSFNHAQVFPLV
                     GVHATQPCAACHVNNVYAGTPRDCAGCHLDDYQRTRDPNHAAAGFPTACQSCHSASAS
                     SWDASFNHNQFFALVGRHAQAACAACHQNGRYGGTPRDCHGCHRADYDGTRDPNHRSA
                     GFPTSCESCHRASDSSWERGRFTHPWFPITSGDHAGIPCASCHTGGSYSSFSCTRCHE
                     RGETDDDHGGVSGYRYDSQACYACHPNGRKD"
     gene            complement(16238..17146)
                     /locus_tag="PKK95_03355"
     CDS             complement(16238..17146)
                     /locus_tag="PKK95_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17276.1"
                     /translation="MDRTAPATLRPPGRDDWLRPSSLSNVLVVSAAATAAVAWLILVV
                     ADGWDYYGTPLRVRAYAPAHPMLRPSGWLGHSLGMAGLLMMAVPVIYSVRKKWKRLAH
                     LGQLRHWLNVHIFCGIFGPVLVTFHAAMKFNGLIAVAYWSMVLVVLSGFVGRYLYVRI
                     PRTIRGVEMGYEEILARANQLRSELAATGLDAALVRPLVPEVSAERGSRLLALDEPLR
                     QTLARRQARRALLHAGAAPDLVREALSLSRERSFLLRRLRYLERTRRLFAMWHVFHQP
                     LVYVMFLIAALHISLAVYLGYAFMPW"
     gene            complement(17148..>17609)
                     /locus_tag="PKK95_03360"
     CDS             complement(17148..>17609)
                     /locus_tag="PKK95_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOC17277.1"
                     /translation="AAILQLVSEPAGCLFMLCGPLPMIAAMRETLVGMGVPAAQVRSE
                     AFEAAVANASAASTADATEAAGAVRPQVSFSLARRVVEAGPGESLLEVAERGGVGIPS
                     VCRAGVCGTCRTRMTSGDADCASDLLGDDERSDGWVLPCVTTARGDCELEA"
BASE COUNT         2604 a         6175 c         6341 g         2489 t
ORIGIN      
        1 caccggcacg tcctgcacct ccagcaccag caccttctgc acctccagca ccagcacctc
       61 ctgcaccagc acctccggca ccagcacctc cagcaccgca cctcctgcac cgcacctcct
      121 gcaccagcac ctcctgcacc tccagcacct cctgcacccc cagcacgact gaactgcgct
      181 atccttcccc ccttcacccc tcccgcgggc cccctgagat cccgggtgga cagcagatcg
      241 gagcaatcga tgcggaagag catgttgtgg ctggcgtgtg cgggggcggt cgtcgcgggc
      301 ggcgccgcgg tgggggcgca gatggcgccc tggctgcagt ggacccttct gccccgtgcg
      361 gcgtggaacg agatcgcggg cgaggcgtcg ggtgagaacg cgtggaagat ggtgatggag
      421 accggcgggt atgaccgcga ccgctcggcc gccgagtacg cgggcacgtt ccgcgaggcc
      481 cagttctatc tcgacaaggc gcgcgagtac ggcctcgagg atgccgagat cgtgcgcttc
      541 ccgggcgggc gcacctggga cgccgtcaag ggcgaattgt gggaggtgac cccgaagcgc
      601 cagaagctgg cgtcctatct cgattcggcg gccatgctcg cgacgggcag cgcgaacacg
      661 gacgtgaagg ccgagctggt ctgggtcggc gcgggacgcc cgtccgacat cgagggaaag
      721 gacctggccg gcaagatcgt ggtgaccgag ggcagcattc ccgccgtgta ccgcctcgcg
      781 tgcgtcgatc gcccggcggc cggcgtggtg ggcgtcatga actcgccgcg gccgctgttc
      841 gacccgctcc agatggcctg ggcgtcgatc gtcggccgcg gcccggcgca ggagtcggag
      901 gccgttgcga agttcgcctt ccagcttccg ccgcgcgagg gcgacctgct gaaacggcgc
      961 ctgctggcgg gggagaagat ctccgtccac gcccaggtgg tcgccgagca ccgcgactac
     1021 accatccagg acgtcgtctg gtcgatcccg ggcaccgacc cgtcgaaggg ggaggtcatc
     1081 ctgtcggcgc acctcttcga gggctacgtg aagatggggg gcaacgacga catctcgggg
     1141 tgcgcggccc tgctcgagat cgcgcggacc ctgaacacgc tcatcgccga ggggcgcctg
     1201 ccccggcccg cgcgcaccat ccgcttcctc atcggccccg agttctcggg caccggcccg
     1261 tgggtgaagg ccaaccgggc gctgatggag aagaccctct gcaacatcaa ctacgacatg
     1321 gtcggcctcc gactcagccg cagccagtcg ttcatgtgcc tgatgcggac gacctacggg
     1381 aacccgcact acatcaacga cgtgatggag aactactacc ggttcgtcgg cgaggggagc
     1441 cgggagcgga tcccgaccaa cgcgccctcg cagcgcctgg tcgccccgac cggatcggac
     1501 gagcccttct actacaacat cgaggtgcac tacggcgcgt cggaccacga ggtgttcaac
     1561 gactggggcg tgcaggtgcc gggggtcatg gtgatcgact ggcccgaccg ctggtaccac
     1621 acctcggaag accacgtcga caagaccgac ccgacgacgt tgaagcgggt ggtcgccatc
     1681 ggcgcgggag cggcctacac gattgcgtcg gccgacgacg agatggcgat ccggatcgcg
     1741 ggggaggtgg cgtcgaatgc gacggccagg ctgcgccacc aaggggcgcg cgcgatcgag
     1801 gaactgaatg gcgcgaccgc cgagacgctt cccgacgcct accgcgcggc ccgaggctac
     1861 gtcgaggcgg cgctgatcaa cgagaaggag acgctcgaga ctgtgaacga gctggcgggg
     1921 gacgccaagc gggtagccct ccacctcgcc gcgctgcaac acggtgtgga gcaggccgcc
     1981 tcggcgcagc tctcgctggt ggacgcacaa atgcgcgccg ccgccgcccg gctcaaggtg
     2041 gcaccggttg cactgaagcc cggcgagctc gaggtgaagg cgtcgaaggt cgttcccagg
     2101 ccgaccgcgc tcgtcactgc cgacggctac cagggttggc gcgaagcact cggcaaggtg
     2161 ccgcaggagc agcgggccaa gttcccgatc ggccggctcg acaccggtga gttgcagcgg
     2221 ctggtgaacg gccggcgcag cgcgctcgac atcaagaaga tgctggacgc ccaggccgag
     2281 acgaaggccg acctccagca ggtgctcaac tacctcgagg tgctcgcgct cgcggggctg
     2341 gtggagaagt aggcggaacg atcgtctggc gcctcgcgga ccgcctcctc gagcgtctgc
     2401 agccaggctt cggggctggc cgaacgggcg atgcgggcac ggggcaccga ccgcgtcact
     2461 gcgaccggcc cccgcctcga ccctgcgggc acgagaaccg ggacgtccac tcgtttcagg
     2521 agatccaggt cggcgtcggc cgccccgacg ccgaccgtca gcaccggccg ccctctcgcg
     2581 tagagtcccc gcagaagccc gacgcaggcc cccatgtcgg ccgacccgac ggcgtggtcg
     2641 aagcgcccct cgctccagac ctggaggccc gcgccgcgca gcgtcttgaa caggcgggcg
     2701 cgagccgccg cgtcggaatc gagtacccgg aacggctcgt cgtactcgcg cagcttggcc
     2761 agcctggctt gcagcagggt caggccggtt tcgcgggcca cctcgctgat cgacatgtcg
     2821 ctgaagccca ccaccgagac ccgcgcctgg ttcgccgcgc gcctgagcgc gcgcacgacg
     2881 gcgtcgtgcg cgatgctccg ctcgaaggtg tggtagcccg accgcgtgcc gctggcaggg
     2941 agcgggaacg ggaagtagcc gcagggcacg aagacggctg cgccgttctc cgcgatgaac
     3001 ggatcggtca gccccagttc gtcgcggagc agctcgacct cggcacgtgt cgcgcgcgat
     3061 gccaccacga ccgggatgcc ccgggcggac agccgcccca ggccctcgcg cagcgtcacg
     3121 cgggcgtgcg ccgagtcgtc gagcaccgcc gggtcaaggc ggctgaagac gacgaccgat
     3181 tgaccctgac cccggccctc ggtgctcgtt cgctcgtccg tgcgttccag gctcgccccc
     3241 gcgctcctca tccctgcctc cgtcccacga gtgtcggccg cgccgtcccc ccggtgtatc
     3301 cggtgaacag ggggttttgg gatgaacgac aggggagagg gcccctgtcg taagatctca
     3361 cccatggaca cgaccgcgag ggaggaggtg cggagggcgc ggcgccaaca ggcgagccag
     3421 gctgtcggcg gcccctccat cggccgcctg ctcctcggcg gcttcgtcct catcttcgcg
     3481 ctctggctgg tgtcgggttt cgacctcgtg aggcgcctga acgacgtcga ggagcggcag
     3541 gcggccctca ccaccgcctt ccggcgggcc gagcggtcgc tcaacgccat ccaggcgcag
     3601 gtccttctgg gctcggtcta cctgcgcgac gcgctgctcg acctcgaccg cgactccgca
     3661 gacgactacc accagcgcct cgagcagagc cgcgccgaca tcgagcaggc gctggcacag
     3721 taccgcccgg tggtgcagtc ggagaccgag cgcgcaagct tccgcacctt gcaggccgag
     3781 gtggacgact actggaacag cctcctcgaa ccggtgctca cctgggacgc cacgcggaag
     3841 gcggtcgagg cccgggccct gctgcgccgg caggtcattc ccaagcgcga gctgatcgcc
     3901 cgcatctcgg cgcgcatcca ggagctcaac cgcgaggcgt tcgagctgca gcaggccgag
     3961 gtgtcgcaga tcttccgcca gctccacacg cgggtctgga cgaccagcgg cctcacgctc
     4021 ctgatgagcc tggtggtcgc cggtgcggtc acccgctacg cgaccatcct cgaggaccgg
     4081 atccgccagc agatggtccg cgacctcgaa catgcccgcg acctgcagcg gctctcggcc
     4141 aagctcgtgg acgtccagga gcaggagcgg cgctccattg cgcgcgaact gcacgacgag
     4201 gtggggcagg cgctcaccgc catcaaggtc gagctggccg tcgctgagcg caccggcggc
     4261 ctctccgaga aggtgcagct cgcgctgcag gacgtccgcg cgatcaccga ccggaccctg
     4321 cagcaggtgc gcgacctctc gcagttgctc cacccgtcgc tgctcgacga cctgggcctg
     4381 ccggcggcac tggagtggta cctgaagggg ttctcgcgcc ggaccgacgt caaggccgag
     4441 ctgctccagg accggatgga gcagcggctc ccgccgcagg ttgaaacctg cgtcttccgc
     4501 gtcgtgcagg aggcgctcac caacgtggcc cgccacgcca gcgcctcctc ggtccgcgtg
     4561 tacctgcagc gcctgccgca cacggtgctg ctgacggtcg aggacgacgg ggtggggctg
     4621 gcgcaggagg acgaggccga gccgaagcgc ggcctgggcc tgctcggcat ccgcgagcgg
     4681 gccggcgaac tgggcggcac cttcaggatc gagagccagc ccgggaaggg aacccgcctc
     4741 accgtcgagc tgccggtcga ggcctcacaa gacgagttct cccatgccga tgcgcatcct
     4801 gctggctgac gatcacacga tcgtccgcca cggccttcgc aagatcctcg aggagagccg
     4861 cgactggcag gttgtcggcg aggccgggaa cggccgcgac gccgtgcggc tggccctcga
     4921 gctgcagccc gaggtcgtca tcctcgacat cggcatgccg ctgctgaacg gcatcgaggc
     4981 cgcccgccag atcgccaggc gcctgccgga cacgaagatc ctgatcctca gcatgcacgc
     5041 cgacgaggcc tacatcatcc aggccctcga ggcgggggcc aagggctacc tgctgaagga
     5101 cacggccgat gccgacctgc tcggcgccgt gaccgccgtg atccgcggca agtcgttctt
     5161 cagcccggcc gtcgcccgcg tcatgctcga cgactacgtc cggcggctgc gcgatcgcgg
     5221 catcgtggac cggtacgaca cgctctcgga gcgcgagcgc gagatcttcc agctgattgc
     5281 cgagggaaag accaacaagg agattgccga gatcctcggc gtgagcccga gcacgatcga
     5341 aacccaccgc gggcacatca tggagaagct cgacgtgcac agcgccgtcg agatggtgct
     5401 ctacgcggta cggaagggca tcatttccac gtaacggttc aatgatccat ccccaccacc
     5461 tcgtcatcgc cgccgaactc tccgtccagc cgcgccaggt ggccgccacc gcggctttgc
     5521 tgggggaggg ggccacgatc cccttcatcg cccgctaccg gaaggaggcc acgggcaacc
     5581 tcgacgaggt ggccatcact cgcgtccgcg accgcctcgc ccagcttggc gagctcgacg
     5641 aaaggcgcgc cgcgatcctg gcctcgctgg aggagcgcca gctcctgacc gacccgctca
     5701 aggctgcggt ggaggccgcc gcgacgatgg cggccctcga ggacgtctac gcgccttacc
     5761 ggccgaaacg gcgcacgcgg gcgacgatcg cgagagacgc cggtctcgag ccgctggccg
     5821 cggtgatctt cgagcggcag gaggaaaccg atccccaggc ggccgccgcc gccttcgtcg
     5881 accccgcaaa gaaggtgccc aacgtcgatg cggcgctggc cggcgcacgc gacatcatcg
     5941 ccgagcaggt ggccgaaacg gccgcggtcc gtgagcggat gcggcggttg ttctggcaga
     6001 gcggcaccgt gcattcgacc gtggtgcccg ggaaggaaac cgagggggtc aagttcaagg
     6061 actactacga ctggagcgaa ccggtcgcgt cgatccccag ccaccggatg ctcgcgatcc
     6121 ggcgcggcga ggccgaggga gtgctgcgcg tgcgcgtggc gcctcccgag ggcgaggcgc
     6181 tgtcggccgt cgaatcgttg accgttcgcg cccccggccg tccagccgcc gagcaggtgc
     6241 ggcttgccgc ccacgactcg tacaagcggc tgctcggccc cgccatcgag ggcgagatcc
     6301 gcttcgactc gaagaagaag gccgacttcg aggccatccg cgtcttcgcc gacaacctgc
     6361 gccagctgct gctcgcgccg ccgctcggcc agaagcgggt gctcgccatc gatccagggt
     6421 tccgcaccgg ctgcaaggtg gcctgtctcg acgcccaggg caagctgctg caccacgacg
     6481 tcgtctaccc gaccgccgcg tccgccgcgc aggcgcggca cgcgggcgac accctgcggg
     6541 ccctcgtcga gcgatttcga atcgaggcca tcgcgatcgg caatggcacc gctgggcgcg
     6601 agaccgaggc cttcgtccgc ggcctcggcc ttcccgtcgt cgtcgccatg gtcaacgaga
     6661 gcggcgcctc ggtgtactcc gccagcgagg ccgcccggga ggagttcccc agccacgacg
     6721 tgacggtgcg gggcgccgtg agcatcgggc gccggctgat ggacccgctc gccgaactgg
     6781 tgaagatcga cccgaagtcg atcggcgtcg gccagtacca gcacgacgtg gacccgctcg
     6841 ccatcaagcg cggcctcgac gacgtggtgg agtcgtgcgt gaacggggta ggcgtggagc
     6901 tgaacaccgc cagccgccag ctgctctcgt acgtctcggg cgtcgggccg tcgctggccg
     6961 cgaagatcgt ctcgcatcgg gatgagcgcg ggccgttccc ctctcgccgc gccctgctcg
     7021 aggttccgcg cctcggcccc aaggcgttcg aacaatgcgc ggggttcctg cgaatccgcg
     7081 gcggcgagca cccgctcgac gcgagcgccg tccaccccga gagctacggg gtggtggacg
     7141 cgatggcccg cgacctgggg tgcagcgtgg ccgacctgat gcgcgacgca ggcctgcgcc
     7201 agaagatccg cctcgagcgg tacgtgaacg agcgcgtcgg cctgccgacg ctgcgcgaca
     7261 tcctcgacga gctcgccagg cccgggcgcg accctcgcga ggagttcgag gcctggcggt
     7321 ttgccgaggg cgtggagaag atcgaggacc tcgagccggg catggtgctg ccgggaatcg
     7381 tcaccaacgt gacggcgttc ggcgcgttcg tggacatcgg cgtgcaccag gacggcctcg
     7441 tccacgtgag ccagctcgcc gaccggttcg tcaaggaccc gtccgaggtg gtgaaggtgc
     7501 agcagcaggt gcgcgtcacc gtcctcgagg tggacctgcc gcgccggaga atcgccctct
     7561 cgatgcgaac cgccccggcg gcggagcggc ggcgcgacga tcggcgctcc gcggcgccgc
     7621 gcccggcggc ttcgctccgc ccgaactcgc ccggggagct gcggcggcca gcggaacgtg
     7681 cccggccggg agggttcaac aacgcgtttg ccgacgcctt caggcggaag ggctagacgg
     7741 catggttcgc caagcctggc gccgcctcgc gatcgtcctg ctcggcctct tgctgtcagc
     7801 cggcggcggc gacgccaaag ctggaagggt gctgcgcctg gcgacgacga ccagcaccga
     7861 ggacacgggg ctgctccacg cgatcctccc gtccttcgag cagcgctgcg ggtgccgggt
     7921 ggacgtcgtg gccgttggta cggggcaggc gatcgccctg ggccgacggg gcgacgcgga
     7981 cgtcctgctg gtccacgcgc gcgcgctcga ggagcagttc gtcgccgagg gccacggcgg
     8041 cccccgccag cccgtgatgt acaacgactt cgtgatcgtc ggaccggccg gcgacccggc
     8101 gcacgccgcc agggcccgga cagcggcgga ggcattccgg cggatcgccg cccggtccgc
     8161 cgcgttcgcc agccggggcg acaagtcggg aacccacgtg gccgagcagg caatctggca
     8221 ggcggccggg gtgtgccacg aaggcagctg gtaccggtcg ctggggcagg ggatgggtga
     8281 gacgctgacg ctggcgaacg aggtgccggc gtacaccctg accgaccgcg gcacctggct
     8341 ctcgatgcgc ggccggctgg acggtctccg actgctcgtc ggcgggaaga accccgcgga
     8401 gaacgaggac ccttcgctgc gcaacgagta ctcggtcatc gtcgtgagcg ccaggaggca
     8461 cccgggcgtt gccgaggggc tggcgcagca gttcgccggg tggctgcgtt cgcccggcac
     8521 ccagcaggcc attggccgct tcggcgtcga gcggtatggc caggcgctct tccaccccga
     8581 cgccggccga cagcgatgac cgaccagttc gcgagtcccg agctgctgcg catcgtcgcc
     8641 ctcacgctgg aagtgtcggg cgcggcgctg ctcattgcga cccttgcggg cgtgccggtg
     8701 ggcgcgctgg tgggcttgct ccacttcccg ggccggcggc tcgtgacgct ggcgctctac
     8761 accggcatgg ggctgccgcc cgtcgtcgtc ggcctcttcg tctacctgct gctgtcgcgg
     8821 agcgggccgg ccggggggct cgggtggctg ttcacgcccg cggcgatggt cgccgcccag
     8881 gcgatcatcg cgttccccct cgtcgccggg ctgacgatga gcgcggtgtc gggcgtcgag
     8941 cgcaccgtgt accagcaggt cgtcgcgctc ggggcctcgt cgtggcagac cggttggacg
     9001 gtgctgcgcg aggcgcgcat cggcgtgacg gccgccatcg tcgccgcctt cggtgccatc
     9061 atttcggagg ttggcgcggt gatgatggtg ggcggcaaca tcgagggcca gacccgggtt
     9121 ctcacgacgg ccatcgtgct gcacacccgg cagggggagt tcgcgatggc ctccgcgctc
     9181 ggcctcacgc tgatctcgct cgcgttcgtg gccaacgcgc tgctgctgcg gctgcagggc
     9241 ggcgtgaggg accgctgatg gcgcgcgacg tggtctaccg cctcgagtcg gtgcgacagg
     9301 aatacgacgg gcgcgtggtg ctcgacatcg accgcctcga catcgcccgc ggcgagaccc
     9361 tggcggtcat cggccccagc ggttccggca aatcgacgct gctccggctg ctccagttcc
     9421 tcgaggcgcc gtcggagggg cgggtgtcct tcgagggccg cgagatgaac ggccgcgtcg
     9481 ccctcgcgac gcgccggcgg gtcagcaccg ttttccagcg gcccatcgcg ctgacgcgct
     9541 cggtcgaggc gaacgtggcc tacgggatcg agctgcgggg acgccgcccc gacgccggcg
     9601 agatccgcgg gctgctcgag gcgctcgacc tggcgcctct cgcccggagg cccgctgcct
     9661 cgctctcggg cggtgagatc cagcgcctgg cggtggcgcg ggcgctggcg acccgtcccg
     9721 acgtgctgct gctggacgag ccgaccgcga atctcgatcc gcgcaacgtg cggctcgtcg
     9781 agaccctcgt gcaggagcgc cgcgcgctgg ggatgacgat cgtgctggcg acacaccaga
     9841 tcttccaggc gcgccgcctc gccgaccgga tcgcgctgct gctcgacggc cgcatcgccg
     9901 aggccggccc ggcgcaggcg ctgctcgatc gcccggccga cgcgcggacc cgcgcgttcc
     9961 tgtcggggga gatgatctac tgatgcccgt gagaacccgc cgcctgcgcg tgatcgtgtc
    10021 cgccgccgcc gtcctcgtcc tcgtgccgct cccgctcctt gcccaggccc ggggcctgcg
    10081 agccatcgag gtcgaggaca tgcagcgctg gctgcggttc ctcggctcca aggagttccg
    10141 cggccgagcc gccccctcga tcgagctcga tctcgcgtcg cgctacctgg cggtcgaggc
    10201 ggcgcacgcc ggcctggagc ctctgctgcc cggaggctcg ttcctgcaac acgtacccgt
    10261 ggaggtgacg acgatctccg gggaacgctc gcgcatccgg ctgtcgtcgc cgggcggcga
    10321 gctgcgcctg gcgttcccgg ccgccttcac cagcagcgtc cagctgccgg gcgactggtc
    10381 ggcgtcgggc ccgcttgtct tcgctggcac cgcgctcgcc ggcccacagc cgaagtggga
    10441 cgacgcggtg gcgagccttc ggggcaggtg ggtggtcgtg ttcgaggagg acgggcgggc
    10501 gcggacggcg gacgagcgcc gggcagcctc ggccgcccgc gaccagctgc tgcgggaccg
    10561 gggggcgctc ggcctcatcg tgatcatcgc gcctgatcgc gaggcgcggc tggcggcgca
    10621 gggcctcgac ttcgacgtgt cgcggcgcct tcggttcctc gacgtggaca ccgtgaaccc
    10681 gccgccgccg ccgctacccg gtgcggcgcc gccccccgtc gagccgatgc cggtcaacgc
    10741 cgtcgaggtg cgccaccagg cggccgcgga catcctcggc gtcacaccgg aggaggtggc
    10801 gaggatggcc cacgacgcct cggagaaccg ggtggtgccg ccacgcccgc tgccgggacg
    10861 gtcggccgac gtcgagatct tcttcgagac ccggcaggcc accaccccga acgtcgtggc
    10921 gtggctgccc ggcagcgatc cgaagctgcg aggcgaatac gtgctgatcg gatcgcatca
    10981 cgaccacaac ccgccgcgcg aggggcgagt gttccccggc gccgacgaca acgggtcggg
    11041 ggcggtgggc atgctgtcgc tggcccgggc gctcgtggcc gagcggccga agcggtccgt
    11101 cgtcttcgtc tggcacacgg cggaagagcg cgggctggtc ggggcctact acttcgtcca
    11161 gcactcgccg gtgccggtcg agaagatcag cgccgtcctg aacctcgaca tgatcagccg
    11221 caacgacccg aacgcgattt acctcatcgg gtcgaaccag gtcagctccg agctggatgc
    11281 aagcctgcgg cggatgaacg accgccacgt gggcctgtcg ctcgactaca agtacgagga
    11341 tccgggcgag ccgagccgct tcttcttccg gagcgaccac tacccgtaca tccgctacgg
    11401 tatcccggcg gtctggctgt tctgcgggac gaccgaggac taccaccgcg agggcgacat
    11461 ggaggagaag gttgactacg cgaagatgga gaaggtggtg cgcctcgcct acctggtcgc
    11521 gatggacgtc gggaacaagg caggactgct ccagctcgac gtgcacccgc agatcaaggc
    11581 ccgcggcgcg cacaacatga aggtggtgtg gcgccgccgg tagcctcaga accggtagct
    11641 gatcccggac agcagcctcg tctgcgtggc gtcgtcgtcg aggacgcgct cgagcgtcgc
    11701 gacgagggac atgcggcgcg gcaggcggat cgtgctcgag agcgaggcac ggcgggtgcg
    11761 agggcgggtc tcgatggcga agccgtcggc cgacacgaac cggagcaccg agagcgacga
    11821 ggcgtaatcg ccggtgaggt agatgctgct gccgagcgag cggcccaccg agacgtacca
    11881 ggagttgaag gagcggccgt tccccccttc catgcgcgag tcggacgcgc tgaggtcgat
    11941 gcccgttccc gcgaggtcgg agacgaagaa gccgtaggtg atccggtcgg tgctccgatc
    12001 ctggcggttg ttcctgtctc gcccgtaacc cccgtagacg cggatgcccc gggccagcgt
    12061 cacggtgagc cttccggagg ccgactcgaa gcggaggccc tcgagcgcgc ggggcagcac
    12121 cggccggccg tccagctggt cgcggagcag ggtgcgggca tcgatcgagc ggccgcggtg
    12181 gtagctgccc tgcagctcca ggaacgagct ggcggtgtag cgggcggtgc cgaacaggta
    12241 gccgagcgtg ccgctgccgg aaacgccagg cccgcccatc tcgtactgtg ccgcctggta
    12301 gacgaacagc ttcgggccga ctggcaggaa gttggtggcc accagtaccg agcgttcggt
    12361 cctgccgcgg tcgcgcaggt acacgtaacc gagcacgtgc cggcgggcgc cggtcccgtc
    12421 gaaggcgacg aggccgccag ccttggtcac gtcgcgctcg tagcccgcct ccaggatctt
    12481 cggctcgagg ccgccgaaga caccggcccg ccagcgcccc cgcccggtcc gcgccttctg
    12541 tcgcagctcg acgaggccgc ctcccaccga gccgaggcct ccgaggtcgt tcaaccacat
    12601 ctgcccggcg cggatcccga tgcggccctc catgaggcgc gccccgacgt acgcgtcgta
    12661 gaccgacacg cggcgcaccc ggccgccgct tcccgggtag cccgccatcc gcaggtcggc
    12721 gcggtactcg aaggcggcat tctcggcgtt ccgcgactcc accgtgaagg tgccgatcag
    12781 ttccgtgaac gagctcgacg acccggcgtc aggggtggtg gagaccgcgc tgctgaagaa
    12841 ggatacgcgg ccccaggcgg gggtgccctg agccgaggcg cccgtcgcga cggcgagcac
    12901 ccccgcggac aggatcgcca cgcgaagtcg gaggccggcg ttcatggggg tcctcgactt
    12961 tcagtctttg cgtccgttcg ggtggcatgc atagcatgcc tgtgagtcgt agcggtaccc
    13021 ggacacgcct ccgtgatcgt cgtcggtctc tcccctctcg tggcagcggg tgcatgagaa
    13081 ggagctgtag ctgccaccgg tgtggcacga ggcgcagggg atgccggcat ggtcgcccga
    13141 tgtgatggga aaccacgggt gggtgaagcg gcccctctcc cacgacgagt cgctggcccg
    13201 gtggcacgat tcgcagctgg tggggaagcc cgccgatcgg tggttcgggt cgcgcgtccc
    13261 gtcgtagtcg gcgcggtggc agccgtgaca gtcgcgcggc gttcccccgt atcggccgtt
    13321 ctggtggcag gccgcacaag cagcctgcgc gtgccgcccg accagggcga agaactggtt
    13381 atggttgaag ctcgcgtccc acgacgaagc cgaagccgag tggcacgact ggcaggcggt
    13441 ggggaacccg gcggcggcgt ggttggggtc gcgggtgcgc tggtagtcgt cgaggtgaca
    13501 gccggcgcag tcgcgcgggg tgccggcgta gacgttgttg acgtgacagg cggcgcacgg
    13561 ctgcgtggcg tggacgccga cgagcgggaa tacttgcgca tggttgaacg acacgccgcg
    13621 ccacgacgca tcggtggccc ggtggcacgc ctcgcaggag gtggggaacc cggcggcggc
    13681 gtgattgggg tcgcgggtgc gctggtagtc gtcctggtgg cagccgacgc actcgcgcgg
    13741 cgtgccgcgg tagaggccgc cggcgtggca ggccgcacag gcctgggtgg cgtggacgcc
    13801 gaccaggggg aagatgctct ggtggttgaa ggtgccgccg ccctcccacg tgctggccgt
    13861 ctgacggtgg caggcctcgc agtcggtcgg gaacccggcc tcgccatggt tggggttgcg
    13921 cgtccgggaa tagtcctcct cgtggcagcc gaagcagtcg agccgcgtgt tgatgaacgt
    13981 gccgttcctg tggcagttgt cgcagcgcag caccgcgtgc gcgacgttca gggcgtaccc
    14041 cgtcctcgcg gcgtggttcc aggtcaccgc cgtccagctg gtcggccggt ggcactgctc
    14101 gcactggctg ccgagcctca ggtggtagcg gtcatcctgg cggcgcgccc agtgacagtc
    14161 gaagcactgc gtcggtgttc ccttcgtcat gccgctcacg tggcagctcg cgcacggcac
    14221 cgtcaggtgc cggccctcga gcgggaacag gcccgacttg tgaaacgccc gcgacgcggt
    14281 ggcccagcgc gcgggctcgt ggcaggtctc gcaggacggg ccgagcgcgc cgtgatggac
    14341 gtcttcgtca ccgtggcagg acgcgcacgc tgtgccggtg ccgcggtatc ggacggccgt
    14401 accgcgtccg ccggggaacg cggcttctcc ctggggatgg cagccgcggc agttgagcgt
    14461 ggcgtgggtg ccgacgaaga agcgctcgaa gcccgcccat cgatgggtat acacggggat
    14521 cttgaagctg tcggtggcgt ggcagctctc gcaccgttcc ccgaactgcg cgaggtggac
    14581 gtccttgtgg caggcggcgc aggaggtggc ggtcccgcgg taattgaccc gacccccggc
    14641 gtcggccggc ttgtggcacg cggcgcacgc ggccgcctgg tgccgtcccg tcagtgggaa
    14701 cgcggtcttc gcgtgggaga acgcggaggc cttgaagccc gcatcctcca cctggtggca
    14761 cgtctcgcac gcggcgccga ggccgccgcg gtgcgcgtcc acgtggcagc ccgcgcaggc
    14821 cgtgctgaag cccttgaacc tccaggagcc gatcgcgccc ggcggcgccg acgacgaccc
    14881 ggtgggacgg ccggccacgc gccagacgtg ggggtgcgcc tgccccccgt gacaggcggc
    14941 gcaggaggcg ccctggtgcg cccccctgaa gaaggcggcg agcgccgccc catgttcgta
    15001 ccgggttacc ggcttgaacg cctcggcgcc gtgacaggtc tggcaggccg tcccggcctc
    15061 ccccttgtgc acgtcctggt ggcacgaggc gcacgtgctc gacaggcctc cgaagaggac
    15121 cggccgcccc gtccggcccg cccgcgaggg cgagagcgtc gacgcgctct tgtggcactg
    15181 gccgcaggcg agcgacgcgt gccgccccgt cagcgggaac ctcgtctcct tcgcgtggtc
    15241 gaagggcgcg gcggcaaagc cgttctcctt gtggcaggcc ccgcagtcct gcttgaactg
    15301 gcccttgtga acgtccacgt gacagttggc gcaccggtcg gcccttaggg cgacccgcac
    15361 ggccggctgc ttgtggcagc tcgcgcacgg caccgtctgg tggcggccgg ccagcgggaa
    15421 cgacgtgcgc gcgtgatcca tcttccgcgt ccgccacgac tcgttgtcat ggcaggccgt
    15481 gcaggtcgac ccgagcggcg cacggtgcgg cgagcggtgg cagtcggtgc acgaggcgaa
    15541 cttcacgccg gtgaacgtgc ggttggtgtg gcagcgcgcg cagtccacct tccggtgggc
    15601 gccggccagg ggaaagcgcg tacgggcgtg atcgaagttg ttgcgggcga cagcgaaccg
    15661 cacggccgtc gaatggcacg tcgtgcaggt cggtccgagc gaacccttgt gcgggtcggc
    15721 gtggcaggac ccgcactcgg gccgcagggt aaggaacgat cgcgtcttgt ggcagctcgc
    15781 gcaccggcgc gccagcggcg cgtgcagccc gtcgagcggg aagcccgttt cccgcgcgtg
    15841 atcgaagttc ccctggtcga aggggcgcag ctcggcgtcg agaccggcgt gctcggcgtg
    15901 gcagtcgaca caccggtcgc ctgcgtcgcg gtgcaccccc ttgcgtgcgg caatccgccc
    15961 ggcgatcggc ttgtgacacg agaggcactt cgaggcggtc acctgacggc cgggctcgtg
    16021 gcacttctgg cagttggcaa tcccctcgag cgaggcgtgc gcccgggaca gcggccccgg
    16081 cgacacgagc gccgcgatct gcgcgtgcgc gacggggggc ccggccagga cggccagaag
    16141 gatcaatgtg ccctttgtgc cctttgtggt ccactcgtcg tggacgggtt cttggggccg
    16201 gtgccgcggt tcgtctttcg tggtcgacac gtttgcctca ccacggcatg aacgcgtaac
    16261 cgaggtacac cgccagggag atgtggaggg ccgcgatgag gaacatcacg tacacgagcg
    16321 gctggtggaa cacgtgccac atcgcgaaca gcctgcgcgt ccgttcgagg tagcgcaggc
    16381 gccgaagcag gaacgatcgc tcgcgcgaga ggctgagggc ctcgcgaacc aggtcgggag
    16441 ccgcaccggc gtggagcagc gcgcggcggg cctgtcggcg cgccagggtc tgtctcagtg
    16501 gctcgtcgag tgccaagagg cggctgccgc gttcggccga gacctcgggc acgagcggtc
    16561 gcacgagcgc ggcgtccagg cccgtggccg ccagctcgga gcgaagctgg ttcgcccgcg
    16621 ccaggatttc ctcgtagccc atctcgacgc ccctgatggt ccgcgggatg cggacgtaga
    16681 ggtaccggcc cacgaagccg gaaagcacga cgagcaccat cgaccagtag gcgaccgcga
    16741 tcaggccgtt gaacttcatg gcggcgtgga aggtcaccag cacgggaccg aagatgccgc
    16801 agaagatgtg aacgttcagc cagtggcgca gctggccgag gtgcgccagc cgcttccact
    16861 tcttgcgcac cgagtagatg accggcactg ccatcatcag cagcccggcc atccccagcg
    16921 agtgcccgag ccagcccgac ggccgcagca tggggtgggc cggagcgtag gcgcgcaccc
    16981 tcagcggcgt cccgtagtag tcccacccgt cggcaaccac gaggatcagc cacgcgacgg
    17041 ccgccgtcgc cgcggcgctc accaccagaa cgttcgaaag gctcgacggc ctcagccagt
    17101 cgtcgcgccc gggcggccgc agcgtcgccg gcgcggttcg gtccacctca cgcctccagc
    17161 tcgcagtcgc cccgggccgt cgtcacgcac ggcagcaccc acccatcgct gcgctcgtca
    17221 tcgccgagaa gatcggacgc gcagtcggcg tctcccgacg tcattcgcgt gcggcacgtg
    17281 ccgcagacgc ccgccctgca gaccgacggg atcccgaccc cgccccgttc ggccacctcg
    17341 agcaggcttt cccccggccc ggcctccacg acgcgcctgg ccagcgagaa gctgacctgc
    17401 ggacgcaccg cgccagccgc ctccgttgca tcagcggtgg aggccgccga cgcattggcg
    17461 accgccgctt cgaacgcctc tgaccgcacc tgcgccgcgg gcactcccat cccgactagc
    17521 gtctcgcgca tggcggcgat catcgggagc gggccgcaca acatgaacag gcagccggcc
    17581 ggctcggaga cgagttgcag gatggctgc
//
LOCUS       DAOPQX010000035        17508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3871_length_17508_cov_5.665616, whole genome shotgun sequence.
ACCESSION   DAOPQX010000035 DAOPQX010000000
VERSION     DAOPQX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17508
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_17508_cov_5.665616"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKK95_03365"
     CDS             <1..294
                     /locus_tag="PKK95_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17278.1"
                     /translation="LFSGLAIDAVTRAAGAIVSRGLSVRETETMVRRMLEGPPVPRPK
                     KQADVHTRAAEQDLRLALGTAVRIVRKRKGGTIEVDFGSEDELQRLYEAMTAK"
     gene            316..1350
                     /gene="selD"
                     /locus_tag="PKK95_03370"
     CDS             316..1350
                     /gene="selD"
                     /locus_tag="PKK95_03370"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:352..354,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOC17279.1"
                     /translation="MKRLTELVSCSGUASKLAAGELAQVLGDMPAQADDRVLIDFRTA
                     DDAGVYRIDGDRALVQTVDFFTPIVDDPAVFGAIAATNSLSDVYAMGGRPITALAIAG
                     FPASGLDEGVIRQVFLGGFHVLRGAGVALLGGHTVQDQEIKFGYAVTGLVAPGDVWAN
                     AGAREGDILFLTKPLGTGVISTALKFGRAPAGAVDAAVAVMLELNARGADALHALPAG
                     SVHGCTDITGFGFLGHAAEMAAASGVTLRVEAAAVPVIAGALTLAGNQSGGLRTNESH
                     FGASVSIAPHVDAALIELLFDPQTSGGLLASVDPARADAAAASFAAAGVAAARIGTVL
                     PRAAHSVLVV"
     gene            1771..2196
                     /locus_tag="PKK95_03375"
     CDS             1771..2196
                     /locus_tag="PKK95_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17280.1"
                     /translation="MLPGLGVFAVIVIVLLCVVVLDRGLFKPLSRVMRQREEAIRSAQ
                     EAAAAAAARAAQATAEFEQRTRAAQAEVYREMEENRRLANEQRAELMAATRQQVDAEV
                     AEAGARVKAQAEEARLQLEREADTMADQIVERVLGRKAS"
     gene            2225..2986
                     /locus_tag="PKK95_03380"
     CDS             2225..2986
                     /locus_tag="PKK95_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17281.1"
                     /translation="MTSHVTQTPHRNGTGGWRLLAAAAALAAWALAAPAAAMVQEHAT
                     PAPQEQAAAGHEQPAEGVSPAQPAQPHGTPHDAAAGHGQDEHAGEAHAAESPWALIGR
                     LFNFAILAGSLIYLLRSPFAAYLATRAKTIRADLANAEEMRAAAGRKLTEIEQRMAAL
                     PGEIQALTERGKQEIAAEEARIREAAAAERQRMLEQARREIDLQVRVAERMLRRRAGE
                     LAVQVAAERVKRTIDAADHARLVDRYLNQVGAGSA"
     gene            3159..3701
                     /gene="atpH"
                     /locus_tag="PKK95_03385"
     CDS             3159..3701
                     /gene="atpH"
                     /locus_tag="PKK95_03385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOC17282.1"
                     /translation="MTNRASAARYARALMDVTRSEGGDLDGVERDLAAFVSLVDGEAA
                     LRRVLVNPALPAARKKALVAELLGRASLGPQVSKLLLLLAERDRLALLPDVLDHYRLR
                     LLDLRNVVQAEVTTAVPLEAERIEALRAALAAMTGRQVAMTTRVDPGLIGGVVTRIGS
                     TVYDGSVKRQLERMREALTQ"
     gene            3770..5299
                     /gene="atpA"
                     /locus_tag="PKK95_03390"
     CDS             3770..5299
                     /gene="atpA"
                     /locus_tag="PKK95_03390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOC17283.1"
                     /translation="MDIRAEEISKIIRDQIGSYALDVDVAEVGTVVSIGDGIARVHGV
                     ERAMAGEMIEFPHGVVGIALNLEEESVGTVLLGEYREIKEGDPVRRTGRIISVPVGDE
                     MLGRVVNPLGQPIDGKGPITTERFSPIERIAPGVVDRQPVREPVQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGKDLICIYNAIGQKTSTIAHVVKTLEDAGVM
                     PYTIVVAAAASDPAPMLYIAPYAACAMGEYFRDTGRHALCVYDDLSKHAQAYREISLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDANGGGSLTALPIIETQAGDLSAYIPTNV
                     ISITDGQIFLESDLFNQGVRPAVNVGLSVSRVGGSAQVRAMRQVAGRLRLDLAQYREL
                     AAFAQFASDLDKATQAQLNRGQRLVEILKQPQYEPLPVERQVVLIFAGTNGYLDQVPV
                     PEIASYERELYKFIDTRRPAIFQTLREKKKIEDDLRAEIESALKEFGAQFAEARKAAA
                     A"
     gene            5458..6327
                     /gene="atpG"
                     /locus_tag="PKK95_03395"
     CDS             5458..6327
                     /gene="atpG"
                     /locus_tag="PKK95_03395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOC17284.1"
                     /translation="MPSLIDIRRRIRAVKSTQQITKAMKMVAASKLRRSQERIMSARP
                     YSRQMMKVLQDVAARVDPSAHPLLARPEACEADRILLIVITADKGMSGSFNANIIRAA
                     ANFIVEQGRPVVLGLVGRKGRDFFARRNYPARFELVNFLNKLDYLHGQAIAKEAIAAF
                     TSREVCAVYVAFNEFKSVLQQRVVVQQLLPIPPADLEEGERFAAGTEYLYEPSAQAIF
                     DELLPRYVEQVVFHALLESNAAQNAAQMVAMDNATKNSGEVIEGLTLYMNKLRQAAIT
                     REIIEVVSGAEAL"
     gene            6390..7838
                     /gene="atpD"
                     /locus_tag="PKK95_03400"
     CDS             6390..7838
                     /gene="atpD"
                     /locus_tag="PKK95_03400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOC17285.1"
                     /translation="MDQTPQQRSGRVVAVIGPVIDIEFEGGQLPPIYNAVRVTGESAG
                     TTIDVVAEVEQHLGENRVRAVAMEPTDGMQRGMTATDLDSPISVPVGPETLGRVLNVL
                     GRPVDFPDKPVKAAERWPIHRKAPVLEDQATALEMFETGIKVIDLIEPYLKGGKIGLF
                     GGAGVGKTVIIMELIHNVALKHGGVSVFGGVGERTREGNDLWLDMQESGVIVAGDPSK
                     SRAALVYGQMTEPPGARLRVGLSALTVAEYFRDAEGKDVLLFIDNIFRFTQAGSEVSA
                     LLGRMPSAVGYQPTLLTEMGELQERITSTRKGSVTSVQAIYVPADDYTDPAPATTFAH
                     LDASTNLSRAIVDRGIYPAVDPLASTSRILDPHVVGQEHYDVARQVKQVLQRYKDLQD
                     IIAILGIDELSEDDKLTVARARKIERFLSQPFFVAEQFTGTPGKYVPIADTIKGFREI
                     IEGKHDDLPEQAFYMVGTIEEAVERAAQMKAS"
     gene            7841..8269
                     /locus_tag="PKK95_03405"
     CDS             7841..8269
                     /locus_tag="PKK95_03405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOC17286.1"
                     /translation="MAIPSHLALEIVTPERSITHEEVDEVEIPGADGYFGVLPGHTPL
                     LASLQVGQLWYRKGSEVSYLSVAFGFAEVMPDRVTILAQFAERADEIDVDRAIAERQR
                     AEDDLKRHVADVDMERARISLLKALTRLQVAERGRARTRG"
     gene            8285..9127
                     /locus_tag="PKK95_03410"
     CDS             8285..9127
                     /locus_tag="PKK95_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOC17287.1"
                     /translation="MRLRFCAASHPGVRRDSNEDSYCVRPDLGLFAVADGVGGHAAGE
                     IASRAALDALEQAMADTRGGTPDSEWPFQFQPALGVDGNRLNWGVHLASRRVRTEAGA
                     ARGREGMATTIAAVMIANVDERRDGVRAEWLGPGAPPPETAPATLAHVGDSRIYRWRG
                     GRLDRLTRDHSWVQEQVDAGRLTAFEARRHPRRNLLTRALSGGAAPVTDIGLVPLVPG
                     DRLLLCTDGLNGALRDDEIEAIVGGEYGETPGATACDALVSAANLAGAPDNVTVIVVE
                     IRGS"
     gene            9227..10021
                     /locus_tag="PKK95_03415"
     CDS             9227..10021
                     /locus_tag="PKK95_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17288.1"
                     /translation="MLSNLRALVRYRPLIQSLVARELKARYRGSVLGFFWSFINPLLL
                     LLIYSFVFSFVLPARVEGIQPYALFLFCGILPWTWFSSSILESANVLISGGNLIKKVL
                     FPAEVLPIVTVLANLVHFLFGLPILVFFLIYYQHTPDLAGLFWFPVVLLVQLVLTLGI
                     ALVVSALTVHFRDIKDILSNLVTFWFFATPIIYPMSAMPSPLAKRLLNLNPFTHLAIS
                     YQEILFYPGPFGHWRWLLALGVASLGLFLVGYFLFDRLRDSFAEEV"
     gene            10310..11698
                     /locus_tag="PKK95_03420"
     CDS             10310..11698
                     /locus_tag="PKK95_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC17289.1"
                     /translation="MSEPSYAIEATGVSKVYRRFGNRRQFATLKSALLTGTLLRDLRP
                     EETFPAVRGVSFRVPRGSTYGIIGRNGSGKSTMLKLVAGITKPTEGTVRVDGRISALI
                     ELGAGFHPEISGRENVFINGIMLGLTKKEVARRFDEIVEFAELQDFIDAPVKTYSSGM
                     YMRLGFAVAINVDPDVLLIDEVLAVGDEAFTHKCLDKFADFRRRGKTILLVTHSLNLV
                     EKFCDEVLWLESGRTRGEGDPRAVVSAYISAVAKSEEQLLAAGDARARETVGAGAEAG
                     APAGTAQGAEPGAAAGPAGEEPPADMFKAAEGRWGTREIEILDVALVGSDGERRHVFQ
                     SGEPAALRVSVRALQRVDDFAFGVGIFNAEGACVYGTNTDIEEYRSERLEGEAEVVFH
                     IDSLDLVEGTYKLDVAAHRRSGAPYDYHRSLYTFRVKSRVKDVGIYRPRHRWEFRGDG
                     LEMRVRRPGREE"
     gene            11698..12162
                     /gene="rfaE2"
                     /locus_tag="PKK95_03425"
     CDS             11698..12162
                     /gene="rfaE2"
                     /locus_tag="PKK95_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOC17290.1"
                     /translation="MVLSREEAVRWAEGVRAAGKTLVFTNGVFDLLHPGHVRYLAEAR
                     RQGDALIVAVNSDRSVRAIKGPARPIHPEGERAEVLAALASVDAVVIFDEDDPHAIIS
                     ALQPDVLVKGADWAEDRIIGRDVVEARGGRVVRIAFAEGYSTTSILDRIKSQ"
     gene            12489..14147
                     /locus_tag="PKK95_03430"
     CDS             12489..14147
                     /locus_tag="PKK95_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOC17291.1"
                     /translation="MKHPARVVAAGLGLLAAACFTVAVQSQERPGRRSLSATDFRELR
                     GMDRALVQLERSGELQLRKRQVDTMIPGRVHERLDQFHQGVPVFGADLVRQVESGTTV
                     SVFGTIERGIGIDTTPAIGPGAAIAVFERETGHKASPSLTPKLFVLPKDDGTFALAWR
                     VSQYTKFSAPVLFVNAANGQVELRYDNLQRQSPAIGSGTGVLGDRKKISASLRNGVYV
                     AYDEMRPPQIATFDLGGDLTRVNWLLLRWLDWSWGDVASDTDNVWDDGAVVDAHTYMG
                     LTYDYYYKRFGRKGFDDHEAPIRVIVHGVTRTAGLQLPRDDFYTYAVNAFWLSDCGRD
                     GRGCFYFGDGLPSGYHLIDGGQYYDYLAGALDVVAHEATHALTTYSCNLIYRNEAGAL
                     NEAFSDILATGAEFYYQPEKADYLIGEDVVRPVLAGARAGMRSLADPRSFGDPDHYSR
                     RYLGSQDNGGVHINSLIAGHAFYLAIEGGTNRTSGKRVEGVGGANREQIEKVFYRAFA
                     YYLTSNATFHSARVATIRAAQELYGPASAAARAVTQAWDAVGVS"
     gene            14159..15346
                     /locus_tag="PKK95_03435"
     CDS             14159..15346
                     /locus_tag="PKK95_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17292.1"
                     /translation="MRSRSIFAVIVLASLLAGAAPAAGQDVARYGPAHIAQAAPFYLY
                     PDESKKPLEALKPGTKVTVVEIEGDWLLVEYEDPSHGVRQGYVKSRMVKPGPAPASKA
                     SPSASKSASGQAPKSAAPAATKGTAPSPTSATGRPGQATAAKKPAAPTRGNWWGDRAL
                     VAVGGAYQTRGSSFSETYSHDQYVERATIATSYPAKEAPGFHGSVLVRVWKGLAGGVA
                     LTRTSHSTTGSINGTIPHPFYFDRMRAVTASAPVSHGETAVHAQVAWVVPVGKSMLLS
                     LSGGPSYFSVSQSLVESAPFTESYPYDSAALSTPVVTEPSQSAVGFNVGADFGWFFTR
                     TIGVGAGVRYARARIEYPLHDATVGIDAGGAQAVVGLRVRVPPGAPKKAAPPKPSSPP
                     KRD"
     gene            15684..16826
                     /locus_tag="PKK95_03440"
     CDS             15684..16826
                     /locus_tag="PKK95_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HOC17293.1"
                     /translation="MPFHSLKDLYVDELRDLYDAERQIIAAMPKMIQAASSSDLRQAF
                     QHHLGETRIQVERLDLIFKNLGVEPKGRHCKGMEGILAEANEIVSEGGAPDVADAALI
                     SAAQRVEHYEISSYGSARTFADRLEDRYAGDLLQKTLDEEGRTDKALTSLAETGINQK
                     AAEGKEIRGSHLGYVSVNEYSYGPAPGAFADVRMSGAGSDDLGTLDGFVVDRNTGRPY
                     YLVVDAGNWFVGRRYLVPVGKARFDQTGRSMRLDIDKDTVKKYPKFESEWFDYDEARR
                     REYEGRLIRAFSTSPAPGVAGGFDYETIPEFRQPEWWRPGDTRGYPSGERHRDYPGER
                     PISERTAARERRPDIERPAAERSGSTGSEGRSTIDEPRRGGRDQKW"
BASE COUNT         2679 a         6180 c         6082 g         2567 t
ORIGIN      
        1 ctcttctccg gcctggccat cgacgcggtg acccgcgcgg ccggcgcgat cgtctcgagg
       61 ggcctctcgg tgcgcgagac cgagacgatg gtgcggcgga tgctcgaggg gccgccggtc
      121 ccgcggccga agaagcaggc cgacgtccac acccgcgccg ccgagcagga cctccgcctc
      181 gccctcggca ccgccgtccg catcgtccga aagcgcaagg gagggacgat cgaagtagac
      241 ttcggatcgg aagacgagct gcagcggctg tacgaagcga tgacggcgaa gtagacgacg
      301 acctcgtgaa ctgagatgaa gcgcctcacc gaactggtgt cctgctcggg ttgagccagc
      361 aagctcgccg cgggcgagct cgcccaggtc ctgggcgaca tgccggcgca agcggacgac
      421 cgtgtcctga tcgatttcag gaccgcggac gatgcgggtg tgtacaggat cgacggtgac
      481 cgcgcgctcg tgcagacggt ggacttcttc acgccgatcg tggacgaccc ggccgtcttc
      541 ggcgccatcg ccgccaccaa ctcgctcagc gacgtctacg cgatgggcgg ccggccgatc
      601 accgcgctcg ccatcgcggg ctttcccgcc agcggcctcg acgagggggt cattcgccag
      661 gtgttcctcg gcgggttcca cgtgctgcgc ggcgcggggg ttgccctgct cggcgggcac
      721 acggtgcagg accaggagat caagttcggc tacgcggtca ccggcctcgt cgcgccgggc
      781 gacgtgtggg ccaacgccgg cgcgcgcgag ggcgacatcc tcttcctcac caagccgctc
      841 ggcaccggcg tgatcagcac cgcgctcaag ttcgggcggg cgccggccgg cgccgtcgac
      901 gccgcagtcg cggtgatgct cgagctgaat gcccgcggcg ccgatgcgct gcacgcgctg
      961 ccggccggca gcgtccacgg ctgcaccgac atcaccggct tcggcttcct gggccacgcc
     1021 gccgagatgg ccgcggccag cggggtgacg ctgcgcgtgg aggcggccgc ggtgccggtg
     1081 atcgcgggcg cgctgacgct cgcgggcaac cagtcgggcg gcctgcgcac caacgagtcg
     1141 cacttcggcg cctccgtctc gattgcccct cacgtggacg cggcgctcat cgagctgctc
     1201 ttcgacccgc agacctcggg cggcctgctc gcctcggtcg atcccgcccg ggcggatgcc
     1261 gccgcggcat cgttcgccgc ggccggcgtc gccgccgcgc gcatcggcac cgtcctcccc
     1321 cgcgcggccc actcggtgct cgtcgtctag ccttcggctc ccgaaccggc cggcggcggc
     1381 gtcgcggagc cgcgggccgt tcgcggagaa agccgtgtga agcaggccga tcaggtgcgg
     1441 acaaaacgta ccgcaggcct gcggacaggc ctggggatgg gggccccagg cgcgttcgga
     1501 cggacggtaa gtaagccgag gtgcgttttg ccggcctgcg gaacccggtt ttcggagcga
     1561 atggcctgcg gctccagaaa tgcgccccgc gccgcctccg cgaacgtcgc ccgcccgcaa
     1621 ccggacgact tcgccgactg acttcgcggc ttgcccgccg aagccgcggc ctgccccgcc
     1681 gcagcgcgaa gcgcgaaggc gggacttccc gacttcgtgg actgatctat ggtataagtg
     1741 agtgttttca ctaagttggg aggggcgcgc gtgcttcccg ggctcggtgt tttcgcggtc
     1801 atcgtcatcg tcctgctgtg tgtggtcgtg ctcgatcgcg gcctgttcaa gccgttgtcg
     1861 cgcgtgatgc gccagcgcga ggaggcgatc cgctccgccc aggaggccgc cgcggccgcc
     1921 gccgcccgcg cggcccaggc caccgccgag ttcgagcagc gcacccgtgc ggcgcaggcc
     1981 gaggtctacc gcgagatgga ggagaaccgc cgcctcgcca acgagcagcg cgccgagctg
     2041 atggcggcca cccgccagca ggtcgacgcg gaggtggccg aggccggggc ccgtgtgaag
     2101 gcgcaggcgg aagaggcccg cctgcagctc gagcgcgagg ccgacacgat ggccgatcag
     2161 atcgtcgaac gcgtcctcgg ccgcaaggcg tcctagtcgt gacctcttcg ccaggcatcc
     2221 gctcgtgaca tcccacgtga cacagacgcc gcaccggaac gggacaggcg gctggcgcct
     2281 gctggccgcc gccgccgcgc tggccgcctg ggcgcttgcc gcgcccgccg ccgcgatggt
     2341 ccaggagcac gcgacccccg ccccgcagga acaggcagcc gcgggccatg agcagccggc
     2401 cgagggcgtc agccccgcgc agccggccca gccccacggt acgccgcacg atgccgccgc
     2461 cggccacggc caggatgagc acgcgggcga ggcccacgcc gccgagtcgc cctgggcgct
     2521 catcggtcgc ctgttcaact tcgcgatcct cgccggctcg ctgatctacc tgctgcgctc
     2581 gccgtttgcc gcgtacctcg cgacccgcgc gaagacgatc cgcgccgacc tggccaacgc
     2641 cgaggagatg cgcgccgccg ccgggcgcaa gctgaccgag atcgagcagc ggatggcggc
     2701 gctgcccggc gagatccagg cgctcaccga gcgcggcaag caggagatcg ccgccgagga
     2761 ggcgcggatt cgcgaggctg ccgccgccga gcgccagcgc atgctggagc aggcgcgccg
     2821 cgagatcgac ctccaggtgc gggtggccga gcgcatgctg cgccgccgcg ccggcgagct
     2881 ggccgtgcaa gtggccgccg agcgcgtgaa gcggacgatc gacgccgccg accacgcgcg
     2941 gctggtcgac aggtatttga accaggtcgg ggcgggcagc gcctgaaccc tgtgcctcgg
     3001 cgcagggttc gtagcgcagg gctttagccc tgcggatctg cccgcggcgc aagaaccaag
     3061 cccccatccg ctcgcggccc tgagccctga gccctgagcc ttgagccttg agccttgagc
     3121 cttgagccct gagccctgag cctgttggtg tcgcagagat gaccaatcgt gcttccgccg
     3181 cgcggtacgc gcgggcgttg atggacgtga cgcggagcga gggcggcgac ctcgatgggg
     3241 tcgagcgcga cctggccgcc ttcgtgtcgc tggtggacgg cgaggccgcg ttgcggcgcg
     3301 tgctcgtgaa ccccgcgctt cccgcggccc gcaagaaggc gctggtcgcc gagctgctcg
     3361 gccgcgccag cctgggcccc caggtgtcga agctgctgct gctgctggcc gagcgggatc
     3421 gcctggcgct gctccccgac gtgctcgacc actatcgcct gcgcctgctc gaccttcgaa
     3481 acgtggtcca ggccgaggtg acgacggccg tgccgctgga ggcggaacgg atcgaggcgc
     3541 tgcgcgcggc gctggcggcg atgaccgggc ggcaggtggc gatgaccacc cgcgtcgacc
     3601 cgggcctcat cggcggggtc gtcacgcgca tcggcagcac ggtgtacgac gggtcggtga
     3661 agcgccagtt ggagcggatg cgcgaggcac tcacacagta gggagtcagg aatcgttctc
     3721 tgcgtctctg cgtccttgag ccctgagcct tgagccggtt ttctaagcta tggatatccg
     3781 agccgaagaa atctcgaaga tcatccgcga ccagattggc agctacgcgc tggacgtgga
     3841 cgtggcggaa gtgggcaccg tggtgtcgat cggcgacggc atcgcccgcg tgcacggcgt
     3901 cgagcgcgcg atggccggcg agatgatcga gttcccgcac ggggtggtcg gcatcgcgct
     3961 gaacctcgag gaggagagcg tcggcaccgt gctcctcggc gagtaccgcg agatcaagga
     4021 gggcgacccg gtgcgccgca ccggccgcat catctcggtg ccggtgggcg acgagatgct
     4081 cggccgcgtg gtgaacccgc tcggccagcc gatcgacggc aaggggccga tcaccaccga
     4141 gcgcttctcg ccgatcgagc ggatcgcgcc cggggtcgtc gaccgccagc cggtgcgcga
     4201 gccggtgcag accggcatca aggcgatcga ctcgatgatc ccgatcggcc gcggccagcg
     4261 cgagctgatc atcggcgacc gccagaccgg caagacggcg atcgcggtcg acacgatcat
     4321 caaccagaag gggaaggacc tcatctgcat ctacaacgcg atcgggcaga agacctcgac
     4381 gatcgcgcac gtcgtcaaga cgctcgagga tgcgggggtc atgccctaca cgatcgtcgt
     4441 cgccgcggcg gcgtccgacc cggcgccgat gctctacatc gcgccgtacg ccgcctgcgc
     4501 gatgggggag tacttccgcg acaccggccg ccacgcgctg tgcgtctacg acgacctctc
     4561 gaagcacgcg caggcctacc gcgagatctc gctgctgctg cggcggccgc cgggccgcga
     4621 ggcgtacccg ggcgacgtgt tctacctgca ctcgcgcctg ctcgagcggg cggccaagct
     4681 gaacgacgcc aacggcggcg gctcgctgac cgcgctgccg atcatcgaga cgcaggcggg
     4741 cgacctgtcg gcctacatcc cgaccaacgt catctcgatc accgacgggc agatcttcct
     4801 cgagagcgac ctgttcaacc agggcgtgcg gccggcggtc aacgtcggcc tctcggtgtc
     4861 gcgcgtcggc gggtcggcgc aggtgcgcgc gatgcggcag gtggccggcc ggctgcgcct
     4921 cgacctggcg cagtaccgcg agctggcggc cttcgcccag ttcgccagcg acctcgacaa
     4981 ggcgacgcag gcccagctga accggggcca gcgcctggtc gagatcctca agcagccgca
     5041 gtacgagccg ctccccgtcg agcggcaggt ggtgctgatc ttcgccggca ccaacggcta
     5101 cctcgaccag gtgccggtgc ccgagatcgc ctcctacgag cgcgagctgt acaagttcat
     5161 cgacacgcgg cgcccggcca tcttccagac gctgcgcgag aagaagaaga tcgaggacga
     5221 cctccgcgcc gagatcgagt cggcgctgaa ggagttcggc gcccagttcg ccgaggcgag
     5281 aaaggccgcc gccgcatagg gtgaaccaca gaggcacaga gaacacagag gttcaacacg
     5341 gagagtcacg cggagaaaag acagatttcc ggaatccgtg gacaagcctc tgtgcctctg
     5401 tgtctctgtg gtgaacgtgg acaggaatgc agtgtgacag cgtgacagcg tgacagcatg
     5461 ccatccctga tcgatatccg ccgccggatc cgcgcggtca agagcacgca gcagatcacc
     5521 aaggccatga agatggtggc cgcgtcgaag ctgcgccgct cgcaggagcg gatcatgagc
     5581 gcccggccgt actcgcggca gatgatgaag gtgctgcagg acgtcgcggc gcgcgtcgac
     5641 ccgtccgccc acccgctgct cgcccgcccc gaggcgtgcg aggcggaccg catcctgctc
     5701 atcgtcatca cggccgacaa ggggatgtcg ggcagcttca acgccaacat catccgtgcg
     5761 gccgccaact tcatcgtcga gcagggccgg ccggtggtgc tcggcctggt ggggcggaag
     5821 gggcgcgact tcttcgcccg ccggaactac ccggcccgct tcgagctggt gaacttcctc
     5881 aacaagctcg actacctgca cggccaggcc atcgccaagg aggcgatcgc ggcgttcacc
     5941 tcgcgcgagg tgtgcgcggt gtacgtcgcg ttcaacgagt tcaagtcggt gctgcagcag
     6001 cgcgtggtcg tgcagcagct gctgccgatc ccccccgccg acctcgagga gggggagcgg
     6061 tttgccgcag gcaccgagta cctgtacgag ccgtcggccc aggcgatctt cgacgagctg
     6121 ctgccgcgct acgtcgagca ggtggtcttc cacgcgctgc tcgagtcgaa cgccgcgcag
     6181 aacgccgccc agatggtggc catggacaac gccaccaaga actcgggcga ggtgatcgag
     6241 gggctgaccc tctacatgaa caagctgcgc caggccgcca tcacccgcga gatcatcgag
     6301 gtggtgtcgg gggccgaggc gctgtaggga cttcgtggac ctgacttcgc gacttgtgac
     6361 ttcgcgactt cgtgacttcg tggactgata tggaccagac tccacagcag cgttccggcc
     6421 gcgtcgtggc cgtcatcggc ccggtgatcg acatcgagtt cgaggggggg cagctgccgc
     6481 ccatctacaa cgccgtgcgc gtgaccggcg agagcgccgg caccacgatc gacgtggtgg
     6541 cggaggtcga gcagcacctc ggcgagaacc gcgtgcgcgc ggtcgccatg gagccgaccg
     6601 acggcatgca gcgcggcatg acggccaccg acctggacag cccgatcagc gtgccggtgg
     6661 ggcccgagac gctcggccgc gtgctgaacg tgctcggccg gccggtggac ttccccgaca
     6721 agccggtcaa ggccgccgag cggtggccga tccaccgcaa ggcgccggtg ctcgaggacc
     6781 aggccaccgc gctggagatg ttcgagacgg gcatcaaggt catcgacctg atcgagccgt
     6841 acctcaaggg cggcaagatc ggcctgttcg gcggcgccgg cgtcggcaag accgtcatca
     6901 tcatggagct gatccacaac gtcgccctga agcacggcgg cgtgtcggtg ttcggcgggg
     6961 tgggcgagcg cacgcgcgag ggcaacgacc tctggctcga catgcaggag agcggcgtca
     7021 tcgtcgccgg cgacccgagc aagtcgcgcg cggcgctggt ctacggccag atgaccgagc
     7081 cgcccggcgc ccgcctgcgc gtcgggctga gcgcgctgac ggtggccgag tacttccgcg
     7141 acgccgaggg caaggacgtc ctcctcttca tcgacaacat cttccgcttc acccaggccg
     7201 gctccgaggt gtcggcgctg ctcggccgga tgccgtcggc cgtcggctac cagcccacgc
     7261 tgctcaccga gatgggcgag ctgcaggagc ggatcacctc cacccggaag ggttcggtca
     7321 cctcggtgca ggcgatctac gtgccggccg acgactacac cgaccccgcc cccgccacca
     7381 cgtttgcgca cctcgacgcg agcaccaacc tgtcgcgcgc gatcgtggac cgcggcatct
     7441 acccggcggt ggacccgctg gcctcgacct cgcggatcct cgacccgcac gtcgtcgggc
     7501 aggagcacta cgacgtggcg cgccaggtga agcaggtcct ccagcgctac aaggacctgc
     7561 aggacatcat cgcgatcctc gggatcgacg agctgtccga ggacgacaag ctcacggtcg
     7621 cccgggcgcg caagatcgag cgcttcctgt cgcagccgtt cttcgtcgcc gagcagttca
     7681 ccggcacgcc cggcaagtac gtgccgattg ccgacaccat caagggcttc cgcgagatca
     7741 tcgaggggaa gcacgacgac ctgccggagc aggccttcta catggtgggc accatcgaag
     7801 aggcagtcga acgcgcggca cagatgaaag cgagctgaag atggcgatcc cgtcgcatct
     7861 ggccctcgaa atcgtcacgc ccgagcggtc gatcacgcac gaagaggtgg acgaggtcga
     7921 gatccccggc gcagacggct acttcggcgt gctgccgggg cacacgccgc tgctcgcgtc
     7981 gctgcaggtg gggcagctgt ggtaccgcaa gggcagcgag gtgtcgtacc tgtcggtggc
     8041 cttcggcttc gccgaggtga tgcccgaccg ggtgacgatc ctcgcgcagt tcgccgagcg
     8101 cgccgacgag atcgacgtcg accgcgccat cgccgagcgg cagcgggccg aggacgactt
     8161 gaagcgccac gtggccgacg tggacatgga gcgcgcccgc atctcgctgc tcaaggcgct
     8221 cacgcgcctg caggtggccg agcgcggccg ggcgcggacg cgcgggtagc cccgctgccc
     8281 tgagatgcgc ctgcgcttct gcgccgcctc ccatcccggc gtccgccgcg actccaacga
     8341 ggacagctac tgcgtgcggc ccgacctcgg cctgttcgcc gtggccgacg gggtgggcgg
     8401 ccacgcggcc ggcgagattg cctcgcgcgc cgcgctcgac gccctcgagc aggcgatggc
     8461 cgacacgcgc ggcggcaccc ccgacagcga gtggccgttc cagttccagc cggcgctcgg
     8521 cgtggacggc aaccgcctca actggggcgt ccaccttgcg agccggcgcg tccgcaccga
     8581 ggccggcgcc gcgcggggcc gcgagggcat ggcgacgacc attgcggccg tgatgattgc
     8641 gaacgtggac gagcgccgcg acggcgttcg cgccgagtgg ctcggccccg gcgcgccccc
     8701 tcccgaaacc gcccccgcca cgctggcgca cgtcggcgac agccggatct accgttggcg
     8761 cggcggccgc ctcgaccgcc tcacccgcga tcactcgtgg gtgcaggagc aggtggatgc
     8821 cggccggctg accgcgttcg aggcgcggag gcacccgcgc cgcaacctgc tcacgcgcgc
     8881 gctgagcggc ggcgccgcgc cggtgaccga catcgggctc gtcccgctcg tgccgggcga
     8941 ccgcctgctg ctgtgcaccg acggcctcaa cggcgcgctc cgagacgatg agatcgaggc
     9001 gatcgtcggg ggcgagtacg gcgagacgcc cggggcaacc gcctgcgacg cgctggtgag
     9061 cgccgcgaac ctggccggcg cgcccgacaa cgtgacggtg atcgtggtcg agattcgtgg
     9121 cagctgacgg ccgccggctg acagcgattg cgcacgcctg ctgttcgctg tctgctgtcc
     9181 gctgtcggct gtcggctgtc tgctgtccgc tgtctgctgt ccgctgatgc tatccaatct
     9241 tcgcgcgctc gttcgctacc gccccctcat ccagagcctc gtcgcgcgcg agctgaaggc
     9301 gcgctaccgc gggtcggtgc tcggcttctt ctggtcgttc atcaacccgc tgctgctgct
     9361 gctcatctac agcttcgtct tcagcttcgt gctgccggcg cgcgtcgagg ggatccagcc
     9421 ctacgcgctg ttcctgttct gcggcatcct gccctggacc tggttctcct cgtcgatcct
     9481 cgagtcggcc aacgtgctga tctcgggcgg caacctgatc aagaaggtgc tcttcccggc
     9541 cgaggtgctg ccgatcgtca cggtgctggc caacctggtg cacttcctgt tcgggctgcc
     9601 catcctggtg ttcttcctga tttactacca gcacacgccc gacctggccg gcctgttctg
     9661 gttcccggtg gtcctgctcg tgcagctggt gctcacgctc ggcattgccc tggtggtgtc
     9721 ggcgctgacg gtgcacttcc gcgacatcaa ggacatcctg tcgaacctgg tgacattctg
     9781 gttcttcgcg acgcccatca tctacccgat gagcgcgatg ccgagcccgc tggccaagcg
     9841 gctcctgaac ctgaacccgt tcacgcacct ggcgatctcg taccaggaga tcctgttcta
     9901 cccggggccg ttcgggcact ggcggtggct cctcgccctc ggcgttgcct cgctggggct
     9961 gttcctggtg ggatacttcc tcttcgaccg gctccgggac tcgttcgcgg aggaggtgta
    10021 gggcgggcgg acagcgtagg ggcgaccctt gtggtcgccc gggccgttcg cggagaaaac
    10081 cgtgtggagc aggccgttca ggtgcggaca aaacgcaccg caggcctgcg gacaggcctg
    10141 gggctggggg tccccaggcg cgttcggacg gacggtaagc cgaggggcgt tttgccggcc
    10201 tgcggaaccc ggttttcgga gcgaatggcc tgcggctcca ccggtacgcg taaccgcctg
    10261 tgaggagccc ggctcggaag cccgatgtac ccgcgagtga agtcaccaga tgtctgaacc
    10321 gagctacgcg attgaagcca ccggcgtcag caaggtctac cggcggttcg ggaaccgccg
    10381 gcagttcgcg acgctgaaga gcgccctcct caccggcacc ctgctccgcg acctgcggcc
    10441 cgaggagacc ttccccgccg tgcgcggcgt gtcgttccgc gtcccccgcg gcagcaccta
    10501 cgggatcatc ggccgcaacg ggtccggcaa gagcaccatg ctcaagctgg tcgccggcat
    10561 caccaagccc accgagggca ccgtccgcgt ggacggccgc atctcggctt tgatcgagct
    10621 gggcgccggc ttccaccccg agatctccgg ccgcgagaac gtcttcatca acggcatcat
    10681 gctcggcctc accaagaagg aggtcgcgcg ccgcttcgac gagatcgtcg agttcgccga
    10741 gctgcaggac ttcatcgacg cccccgtcaa gacctactcg tcgggcatgt acatgcgcct
    10801 cggcttcgcc gtggccatca acgtcgatcc cgacgtgctg ctgatagacg aggtgctcgc
    10861 cgtcggcgac gaggcgttca cccacaagtg cctcgacaag ttcgccgact tccgccgccg
    10921 cggcaagacg atcctcctgg tcacccactc gctcaacctc gtcgagaagt tctgcgacga
    10981 agtgctctgg ctcgaatccg gccgcacccg cggcgagggc gacccgcgcg cggtcgtctc
    11041 ggcctacatc tcggcggtgg cgaagtcgga ggagcagctg ctcgccgccg gcgacgcgcg
    11101 ggcccgcgag accgtcggcg caggcgccga ggccggggcg ccggccggta cggcgcaagg
    11161 ggccgagccg ggcgcggcag ccggacccgc cggcgaagag ccgcccgccg acatgttcaa
    11221 ggccgccgag ggacgatggg gcacccgcga gatcgagatc ctcgacgtcg cgctggtcgg
    11281 ctccgacggc gagcgccgcc acgtcttcca gtcgggcgag ccggccgcgc tgcgcgtgtc
    11341 ggtccgcgcg ctccagaggg ttgacgactt cgccttcggc gtcggcatct tcaacgccga
    11401 gggcgcctgc gtctacggca ccaacaccga catcgaggag taccgatccg agcgcctcga
    11461 gggcgaggcc gaggtcgtct tccacatcga cagcctcgac ctggtggaag gcacctacaa
    11521 gctcgacgtc gccgcccacc gccggagcgg cgctccctac gactatcacc gctcgctcta
    11581 caccttccgc gtgaagtcgc gcgtgaagga cgtggggatc taccggccga ggcaccgctg
    11641 ggagttcagg ggcgacgggc tggaaatgcg ggttcgacgc ccagggcgag aagagtgatg
    11701 gtgctctcca gggaggaagc cgtccgctgg gccgagggcg ttcgcgccgc cggcaagacc
    11761 ctcgtcttca ccaacggcgt cttcgatctc ctccacccgg gccacgtgcg ctacctcgcc
    11821 gaggcccgcc gccagggcga cgccctgatc gtcgccgtca actccgaccg ctccgtccgc
    11881 gccatcaagg gacccgcgcg cccgatccac cccgagggcg agcgcgccga ggtgctcgcc
    11941 gcgctggcct cggtggacgc cgtcgtcatc ttcgacgagg acgacccgca cgccatcatc
    12001 tcagccctgc agcccgacgt cctcgtgaag ggcgccgact gggccgagga ccgcatcatc
    12061 ggccgcgacg tcgtcgaggc ccgcggcggc cgcgtcgtcc gcatcgcgtt cgccgagggc
    12121 tactcgacca cctccatcct cgaccggatc aaatcccagt agagcaggcg tccccgcctg
    12181 cgggacaagc aggcgtcccc gcctgcggaa caagcaggcg tccccgcctg cggaacaggc
    12241 aggcgtcccc gcctgcggga caggcaggcg tcccctggca cgccgtagcc gctcttcggc
    12301 gaaggcggca ccctgcgctt gtctcgccga agccgttctt tggcgaaggc ggctgttgac
    12361 gcatgaatgg ctgaggttca tactgccgca gcgcgtcacc ttccggtcat cttccgggct
    12421 tagagcagta ccgtgtcaac ccttcgacag cggagtcgtc cccgccgcga acagcggagg
    12481 atttctctat gaaacaccca gcgcgcgtcg tggcggccgg tctgggcctg ctggccgccg
    12541 cctgcttcac ggttgccgtg caatcgcagg agcgcccggg gcggcgctcc ctcagcgcga
    12601 ctgacttccg cgagctccgg ggcatggacc gcgcgctcgt gcagctggag cgcagcggcg
    12661 agctgcagct gcggaagcgg caggtcgaca cgatgatccc gggccgcgtg cacgagcgcc
    12721 tcgaccagtt ccaccagggc gtcccggtgt tcggtgccga cctcgtgcgc caggtcgagt
    12781 cggggacgac ggtttcagtg ttcggcacca tcgagcgcgg catcggcatc gacacgaccc
    12841 ctgcgatcgg tcccggcgcg gcgatcgccg tcttcgagcg cgagaccggc cacaaggcct
    12901 cgccgtcgct gacgccgaag ctcttcgtgc tccccaagga cgacggcacc ttcgcgctgg
    12961 cctggcgcgt gtcgcagtac acgaagttct cggcgccggt gctgttcgtg aatgccgcga
    13021 acggccaggt ggagctgcgc tacgacaacc tgcagcggca gtcgccggcg atcggcagcg
    13081 ggacgggggt gctcggcgac cggaagaaga tcagcgcgtc gcttcgaaac ggtgtgtacg
    13141 tcgcgtacga cgagatgcgg ccgccgcaga tcgccacctt cgatctcggc ggcgacctca
    13201 cgcgcgtgaa ctggctcctt ctaagatggc tcgactggtc gtggggcgac gtggcgagcg
    13261 acaccgacaa cgtgtgggac gatggcgcgg tggtggacgc ccacacgtac atgggcctca
    13321 cctacgacta ctactacaag cgattcgggc ggaagggctt cgacgaccac gaggccccga
    13381 tccgcgtgat cgtgcacggg gtgacacgca cggccggcct tcagcttcct cgcgacgact
    13441 tctatacata cgcggtcaac gcgttctggc tctccgactg cggccgcgac gggcgcgggt
    13501 gcttctactt cggggacggc ctgccttccg gctatcacct catcgacggc gggcagtact
    13561 acgactacct ggcgggcgcg ctcgacgtcg tcgcgcacga ggccacccac gcgctcacca
    13621 cgtactcctg caacctgatc tacaggaacg aggcgggggc gctgaacgag gccttctccg
    13681 acatcctcgc cacgggggcc gagttctact accagcccga gaaggccgac tacctgatcg
    13741 gcgaggacgt cgtgcggccc gtcctcgccg gcgcgcgggc ggggatgcgc tcgctggccg
    13801 acccccgctc cttcggcgat cccgaccact attcgcgccg ctacctcgga agccaggaca
    13861 acggcggcgt gcacatcaac tccctcatcg ccggtcatgc cttctacctg gccatcgagg
    13921 gcggcaccaa ccgcacctcc ggcaagcgtg tcgagggcgt cgggggcgcc aaccgcgagc
    13981 agatcgaaaa ggtcttctac cgcgcgttcg cctattacct gacgagcaat gccacgttcc
    14041 actcggcccg cgtggccacc atccgcgcgg cccaggagct ttacgggccg gcgagcgccg
    14101 ccgcacgggc cgtcacccag gcctgggacg ccgtgggcgt gtcctaagga gcgatgtcat
    14161 gcgcagccga tcaatcttcg ccgtgatcgt tcttgcatcg ctcctcgcgg gcgccgcccc
    14221 ggccgccggc caggacgtgg cccgctacgg cccggcgcac attgcgcaag cggcgccgtt
    14281 ctacctctat cccgacgagt cgaagaagcc gctcgaggcg ttgaagccgg gaaccaaggt
    14341 gacggtggtg gaaatcgagg gcgactggct gctggtggaa tacgaggacc cgagccacgg
    14401 cgtgcggcaa ggctacgtca agtcgcgcat ggtgaagccg ggcccggcgc cggcctccaa
    14461 ggcctctccg tcggcctcca agtcggcctc cgggcaggcg ccgaagtccg cagcgccggc
    14521 ggcaaccaag ggcacggctc cctctccgac ttcggcgacc gggcgaccag ggcaggccac
    14581 ggccgcgaag aagccggcgg cgccaacgcg cggcaactgg tggggcgacc gtgccctcgt
    14641 cgcggttggc ggagcttacc aaacccgcgg gtcgtcgttc tccgagacct actcgcacga
    14701 ccagtacgtc gagcgcgcga ccatcgccac gagttacccg gcgaaggaag cgcctggctt
    14761 ccacgggtcg gtgctcgtcc gcgtgtggaa gggcctggcc ggcggcgtgg cgctgacgcg
    14821 aacgagccac tccaccaccg gcagcatcaa cgggacgatc ccgcacccct tctacttcga
    14881 tcggatgcgc gcggtgacgg ccagcgcccc ggtttcgcac ggcgagacgg ccgttcacgc
    14941 gcaggtggcg tgggtcgtgc cggttggcaa gagcatgctg ctctcgctat cgggcgggcc
    15001 gtcgtacttc agcgtctccc agtcgctggt ggagagcgcg cccttcaccg agtcgtatcc
    15061 gtacgactcg gcagctctct cgacgccggt ggtcaccgag ccctcgcagt cggccgtcgg
    15121 gttcaacgtc ggcgccgact tcggctggtt cttcacccgg acgattggcg tcggcgccgg
    15181 cgtgcgctac gcccgcgcgc gcatcgagta cccgctgcac gacgccaccg tcggcatcga
    15241 cgccggcggg gcgcaggcgg tggtggggct ccgggtgcgc gtgccgccgg gcgcgccgaa
    15301 gaaggccgcg ccgccgaagc cctcgtctcc gcccaagcgg gactgaggcg cgaggagccg
    15361 caggccattc gctccgaaaa ccgggttccg caggccggca aaacgcccct cggcttaccg
    15421 tccgtccgaa cgcgcctggg gacccccagc cccaggcctg tccgcaggcc tgcggtgcgt
    15481 tttgtccgca cctgaacggc ctgctccaca cggttttctc cgcgaacggc ccgcggctcc
    15541 acctgaacgc agaactccca agctgtgtcc tttacacccg agcactcccg cgtgagatcc
    15601 cccggttgag cacctccctg cgcgcgggca caccttctgc aggacgtggg cctttgggtc
    15661 cgttactgag aagggaagtg ccaatgccgt ttcactcgct caaggatttg tacgtcgacg
    15721 agcttcgcga cctctacgac gccgagcgtc agatcatcgc ggcgatgccg aagatgatcc
    15781 aggccgcctc gtcttccgat ctccgccagg cgttccagca tcacctcggc gaaaccagga
    15841 tccaggtcga gcggctcgac ctcatcttca agaacctcgg cgtcgagccg aagggccgcc
    15901 actgcaaggg catggagggc atcctcgccg aggccaacga gatcgtctcc gagggtggcg
    15961 cgcccgacgt cgccgacgcg gcgctgatct cggccgccca gcgcgtcgag cactacgaga
    16021 tttcctctta cggttcggcc cgcacgtttg ccgaccgcct cgaagaccgg tatgcgggcg
    16081 atctgctgca gaagaccctc gacgaggagg ggcgcaccga caaggcgctg acgagcctgg
    16141 ccgagaccgg catcaaccag aaggcggccg aggggaagga gatccgcggt tcgcacctcg
    16201 gctacgtcag cgtgaacgag tacagctacg gtcccgcgcc cggggcgttc gccgacgtgc
    16261 ggatgtcggg tgcgggcagc gacgacctcg gcacgctgga cgggttcgtc gtcgaccgca
    16321 acaccggccg cccgtactac ctcgtggtgg acgcgggcaa ctggttcgtc ggccggcgct
    16381 acctcgtccc ggtggggaag gcgcgcttcg accagaccgg caggtcgatg cggctcgaca
    16441 tcgacaagga caccgtgaag aagtacccga agttcgagtc ggagtggttc gactacgacg
    16501 aggcgcgccg ccgcgagtac gagggccgct tgatcagggc gttctccacc tcgccggcgc
    16561 cgggcgtggc gggcggcttc gactacgaga cgatccccga gttccggcag cccgagtggt
    16621 ggaggccggg cgacacgcgc gggtacccgt cgggcgagcg gcaccgcgac tacccgggcg
    16681 agcggccgat ctccgagcgc acggccgcgc gcgagcggcg gcccgacatc gagcggccgg
    16741 ccgccgaacg cagcggctcg accggcagcg agggccgcag cacgatcgac gagccgcgcc
    16801 gcggcggccg cgaccagaag tggtgaggag gttcgcctgg aggtagtggc ccgcaaccgc
    16861 gatcgggatc cggggcctgt cggggcagcg acaccgtttt gtccggccgg gcacctcgac
    16921 gcgcgggcga aggtcgccgg ccggcatcag ccggccggac tccctgatga ctcccgcttg
    16981 cgaggctgcc cggctcggcc aaaaggtgtc tggctgcccc gccacccgct ccggttgcgg
    17041 gcgtgagcgc ggagaggctg cctgggctcg aggttgtcgc ggcggcgacg cggacagccg
    17101 gtgcgtcggc gaacgagccg tcgaagagcg ctggcgctgc gcatcgagcc gcgggccgtt
    17161 cgcggagaaa accgtgtgga gcaggccgat caggtgcgga caaaacgcac cgcaggcctg
    17221 cggacaggcc tggggatggg ggcccccagg cgcgttcgga cggacggtaa gccgaggtgc
    17281 gttttgccgc ccttcgacct tgctcagggc gccccgagcg tggtcgaggg gcggcctgcg
    17341 gaacccggtt ttcggagcga atggccggcg gctccagttg cggtatctac accgtccagg
    17401 tgcgccgggc gcggctctcg ccggcgagct tgaccttcag cgtctgcgcc ttgcggtcgc
    17461 ggacgatccc gatctcgatc tccgcgtcat catccgcggc gcggatcg
//
LOCUS       DAOPQX010000036        17438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_3888_length_17438_cov_4.285451, whole genome shotgun sequence.
ACCESSION   DAOPQX010000036 DAOPQX010000000
VERSION     DAOPQX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 17438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17438
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3888_length_17438_cov_4.285451"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PKK95_03445"
     CDS             <1..575
                     /locus_tag="PKK95_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC17294.1"
                     /translation="FNILLQVFEDGHLTDGLGNRVDFKNTIVIMTSNIGARHIQKKGG
                     MGFQSADRAEVNRSLSDLVLGDVRRTFNPEFLNRIDEIIVFDPLSDDDLRRILELLVS
                     QLNEHLADRRLRITLTPEATEWIIAATCQDRSYGARPLRRAIQRYVEDPLSEELIRGR
                     LRGGAVEVYLDGGILAYRPVAETPAGQTLA"
     gene            676..2352
                     /locus_tag="PKK95_03450"
     CDS             676..2352
                     /locus_tag="PKK95_03450"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOC17295.1"
                     /translation="MRKFRLTSIAVALLVLLAAAGFAHAEPPAAQQAPAPLSGTITWC
                     GAQVGPPRNLPPADSGPVLYLLGVCFPEQGGVSVIEPETYQYYIQTKRSLPSQNAWVP
                     WDQSVEESIRGDFKRLWGTNFLDNLSIEVTDYQFSNGVVGKLVAYNMEERQRVKVVEY
                     IGSKKIEQTKVDEKLKESNSVIRLDSFLDPGLIKRVSTTIREMLAEKGYQFATVTPEI
                     KPIGGGPKLVNLTFHIDEGPQVRIRDVEFVGNEAIPDWRLARELKTNKSEWFLSFLTG
                     RGTFQEAKYEEDAENVVGFYRDRGYIAVRVGEPELRVLEDSKDRRTRYVSLRIPVFEG
                     ERYKVGDVAFDGNTVVKPDALRTLFKLRPGEFYSDKAIRKGLEKAREVYGSAGYFEFT
                     GYPDLQPRDEAARARAEGAQGNGPAAGEPAAEGEKPAEGGEAAEAQKPAEGGQLADGQ
                     KPAEGEAAAAGPKPERAPSGEPLVDVTMRLQEGKQYFVNRITFVGNTTTRDNVVRREL
                     RLFENGVFNTEALKYSIKRINQLGYFKALEGAPTSTSRRRRTSRTRWTSR"
     gene            2337..3416
                     /locus_tag="PKK95_03455"
     CDS             2337..3416
                     /locus_tag="PKK95_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOC17296.1"
                     /translation="MDVTLKFEEQNRNQITFGAGISQWEGFFGQLAFQTANFMGRGET
                     LTLSLQAGSRAQNYQLAFTEPYLFDRAITGGIDIFKRELRYISQFTQSSYGGNVLFGF
                     PLRDFTRMFVQYSYENVRVKDINPIFTTPEYIAGNPYLADYLLLGEDNNSRTVSKIVP
                     SLVHNTVDDPIFPKAGTRYTVSVDLAGLGGDTNFYKPRVEGVWYIPHTRRTSVGLRAQ
                     AEYITQIKGNKTLPVFERLYLGGEYSVRGFDIRTIGPKLQNGLVIGGNKSLLLNAEYL
                     ITIAGPVRLVMFYDAGQALDVGQKFSLKEFRTSTGAEIRFFMPVLNVPFRLIFAYNPQ
                     RDGVLDNNLQPQKAFSFKFAVGSTF"
     gene            3489..4100
                     /locus_tag="PKK95_03460"
     CDS             3489..4100
                     /locus_tag="PKK95_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOC17297.1"
                     /translation="MRRLAVAAVSFALAALPTLASAQTAPQAPAQQKPAEVRPFPEGA
                     KVAFMNIQQVASDSAEGKAANAKVQALRDKKLQELQAKNKQLEAAQQKVQGGNLMSEQ
                     ARAQAQKEVDRLTVEIQRAQQDADAELNELSQELNLEFQRKLGPVIQQVSQERGLHML
                     LSRADAGIIWAEPGLDLTGEIIKRFDAATAAGAKPPAAPPKRP"
     gene            4171..5424
                     /gene="lpxA"
                     /locus_tag="PKK95_03465"
     CDS             4171..5424
                     /gene="lpxA"
                     /locus_tag="PKK95_03465"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOC17298.1"
                     /translation="MRPTTIPVLLDRLRYRYPSRLVDAVLEHVPGERVVTAKNVTVNE
                     QFFQGHFPGAPVLPGVLMIEAIAQAAALLLLHGDERLPTARVVLRGVNGAKFRRQVVP
                     GDRVRIEVSRRTGRGTIARVHGEAFVASQLVVEADLLLAIVQDDVSIHPTAVVHPQAR
                     IGHGTVIGPHATIGPRVVIGKDCRIGPSSVIDGCTEIGDGNHLAAFVSIGLPPQDLKY
                     SGEDTRVVIGNRNSIREFVTIHRGTAGGGGETRLGDDNLLMAYTHVAHDCQVGSGIIF
                     ANNATLGGHVHVEDFAIISALSGVHQFCRVGRHAFIGAHTAVTKDALPFVKTVGNRAR
                     VYGLNTVGLLRRGFTHESIGKLKRAYRLLLKLNTGRAVIRIEADPSLQCPEVAYLVEF
                     IRGSSHRGVLLRRPTRRADSEVSDE"
     gene            5431..6252
                     /gene="lpxI"
                     /locus_tag="PKK95_03470"
     CDS             5431..6252
                     /gene="lpxI"
                     /locus_tag="PKK95_03470"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOC17299.1"
                     /translation="MHIGLIAGNGRFPFLVLDAARRLGHQVTVVAVREEASPDLEAAA
                     ATAEPRAAIVWLSLGQLGACIETFRAAGVTHALMAGQIKHVKIFSGIVPDRLLLSALL
                     RLRSKNTDALISAVAEILREHGIELLDSTAFLTPLLAPSGRVAGRHPSADERADLEFG
                     YRMADAIAGLDIGQTIAVKAAAVVAVEAMEGTDAVIARAGRLAGPGVVVVKVAKPNQD
                     MRFDVPVVGLATIEAMRAAGASVLSVDAGKTLLFDREALAAAAAQAGIAVVGRER"
     gene            6249..7190
                     /locus_tag="PKK95_03475"
     CDS             6249..7190
                     /locus_tag="PKK95_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17300.1"
                     /translation="MTGNRVLRVAAIGVGHLGRHHARILGSLEGAELVGVVDAKPDRA
                     REIAASCGTRAVERAADLLGQVDAVSIAVPTEAHLQAAMPFLERGIPVLVEKPLARDV
                     AEADRMIDAAARAGTILAVGHTERHNPAVALAAAHLADPRFVEVHRLGTFPERSLDID
                     VVFDLMIHDLDIVLSLVAAEVAAVEAVGVPVLTGRVDIANARLRFQNGCIANITASRI
                     SRERVRKIRFFQPEAYLSVDYAAQEVEMYRLVRGDGRPAIEGGKLEVQHEEPLRRELS
                     DFLDAVRQRRPPLVSGSDGRRAIVLAQRIVERMAQGN"
     gene            7204..7908
                     /gene="ubiE"
                     /locus_tag="PKK95_03480"
     CDS             7204..7908
                     /gene="ubiE"
                     /locus_tag="PKK95_03480"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOC17301.1"
                     /translation="MIDKRPARVAGMFDAIAARYDLLNHLLSAGLDRGWRRRAIRSLG
                     LRGGETLVDLCTGTADLALAAAAGGAGRVIGVDFAAEMLRLGAAKVRRRGGVALVRGD
                     ATSLPIGSGTADAATVAFGIRNVERPERALAEMFRVLRPGGRAAILEFSIPASPVVRA
                     VYLPYFRHVLPRIGRLVSGHLSAYSYLPASVGSFIAPSDMAALLRRSGFDQAEAASLA
                     LGAVTLYTARKAAADW"
     gene            complement(7875..9005)
                     /locus_tag="PKK95_03485"
     CDS             complement(7875..9005)
                     /locus_tag="PKK95_03485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17302.1"
                     /translation="MRVVHVYKHYYPIIGGIENHVRALAEEQARRGYEVTVMAASPGH
                     RGGERMADGVRLVLLPRLATVCSTPICPTLPSRLRRERPDLIHLHFPYPPGELARLVL
                     GRGTPTVITYHCDIVRQRRLLRLYEPLLRRVLERADRVVVTSPPYLRTSRYLPSISDK
                     CSVVPLGIDVERFAADGRASGQRLRRGWGIAEEAFVVGFVGRMRHYKGLDHLLRAIAR
                     VPAARLLLVGDGPLKRRLQRLAAALDVSERVTFAGEVSERDLPGCYRAADLFVLPSTS
                     RAEAFGTSMVEAMAAGLPVISTELASGTSWVNESGTTGLVVPACDPARLAEAIDRLRQ
                     DPGLRSRMGAAARQRASSLFRLSSMVAELDAVYQSAAALRAV"
     gene            complement(9102..10253)
                     /locus_tag="PKK95_03490"
     CDS             complement(9102..10253)
                     /locus_tag="PKK95_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOC17303.1"
                     /translation="MIPEGLVLDARTVSGHFPGIGRVVLGLAGALSRLGSATLLHAAH
                     PDPRLPVPPLAGVPCGAGPFALRQQWEVPRLLRKAGARVYHSPYYLMPYRPGIPTVVT
                     CFDLIPMLTPGVLGPGRRFGYVLAHRLAFRASAIVCVPSEATRRDVERLFPAARGKLT
                     IVRPGVTWVQAPPGQAADRPAPASGALPAAQWGCPRSVLPPRFLLQVGSNKPHKGLDV
                     LLRAWALALAEAPAQTAGITLVMAGPRDPRYPEPARTIERLGLSTRAADLGPVGDRQL
                     NELYAAALLFVCPSLREGFGLPLAEAMARGRPCVSSDTPALAETAGGAAALFPTGNAE
                     ALAAAILRLLSDEDEARRLGGLALARAACFDWDLAAREMAAVYERVAQG"
     gene            complement(10250..11407)
                     /locus_tag="PKK95_03495"
     CDS             complement(10250..11407)
                     /locus_tag="PKK95_03495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17304.1"
                     /translation="MLAVLHVAHSHQVSEGKIERLRDDAGLRIHLVRSTRTPDRLDGT
                     LRVPVWRRADPHRCLYGTLAFGLRRFHPDIVHVEEEPDSLAALQVALARRRWARRTRL
                     VYHTWQNVRRELGPGARALLALTLRSADAILCASREAERVWRSLGFTGLTRTILPQGV
                     DVSSFAPPPAPPPPGRFSVGYVGRLAAEKGCATLLDAVAALEPPAELTLVGDGPERRA
                     LEVRAGALGLAGRVRFTGTLSHESLPGVFAGFDVVVLPSRTTAVWKEQFGRVLAEAMA
                     CAVSVVGSDSGAIPDVIGDAGLVFPEGDAAALAQRLRTLRESPALRRQLGRRGRERAV
                     AEFSEATLARQTAAFYRELAGKERGGPRTEGGTDSGGWKAKDGTEGAGGVS"
     gene            11456..12451
                     /locus_tag="PKK95_03500"
     CDS             11456..12451
                     /locus_tag="PKK95_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17305.1"
                     /translation="MYFDFEDNHPDISPIGRAISWREGVLLSIIFHLVVILAAVLMPE
                     FPGAREAAARAAEAAELQRQQELERTRFVFVQPRLDVPAPKPPPPRADLSDKDRVAQA
                     PERAQNPTNSLPFSRGNTPEQVDQPAVMAREAPRVESRPEPGPSEEQRAQADQRQGQG
                     GENGTGEANRSALSLPGRRQPASRPGEGTGRPGAPGLFAGAMQNPERYASQRFQNEGG
                     GGGAFGPAIQFDTKGVEFGPWVRRFIAQIKRNWVVPYAAMAFSGHVVLTFNVHKSGAI
                     TDLMVVGPCAVEAFNNSSFGALAASNPTYPLPPEYPSDRAFFTVTFYYNEVPPVQ"
     gene            12565..13518
                     /gene="miaA"
                     /locus_tag="PKK95_03505"
     CDS             12565..13518
                     /gene="miaA"
                     /locus_tag="PKK95_03505"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOC17306.1"
                     /translation="MLVAVLGPTGSGKSALGLALAERFGGEIVSCDSAAVYRGLDIGT
                     DKLPLEARRGIPHHVVDVADPRESYTAARFARDASEAVRAIHARGRLPILVGGTGFYY
                     RALTRGLFPGPSRDEALRERLRAAARRRGAGFLHRMVARVDPGSARRILPRDEVRLVR
                     ALEVYFLTGRPLTAHFADTVSPLPGVEVVGLLLQVPAAVLGERIAARVAGQFARGWVN
                     EVRRLLASGVPPDAHAFQALGYRELIEHLEGRLDERAARELIVAETRKYARRQLIWFR
                     KEPNLEVIPGPGERGTAIAAATRLVARRLPGSPCARDEETP"
     gene            13519..13782
                     /gene="hfq"
                     /locus_tag="PKK95_03510"
     CDS             13519..13782
                     /gene="hfq"
                     /locus_tag="PKK95_03510"
                     /inference="COORDINATES: protein motif:HMM:NF028518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOC17307.1"
                     /translation="MASATEPRPVVTSPNIQDAFLNHARRERLTVRFRLLDGTEFEGR
                     IKNFDRFAIIVDREGADQMIFKHAVATITSSRTVGSYYTHQSQ"
     gene            13779..14990
                     /locus_tag="PKK95_03515"
     CDS             13779..14990
                     /locus_tag="PKK95_03515"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOC17308.1"
                     /translation="MTPPPIRRAIVIVLDSLGIGELPDAAAYGDQGSDTLGHIAACVP
                     LAVPALRSLGLGRLSNLGPQARTPVPLAPQARTPVPLGAFGRMVESAPGKDSVTGHWE
                     LMGLVLDRPFPVFPHGFPADRLEEFERRIGRRTLGNTVASGTVIIEELGPEHLRTASP
                     IVYTSADSVFQIAAHEDVIPIDELYRQCEIAYAMFVEGMGVGRVIARPFVGQPGSFRR
                     TANRRDFAIRPSGPTVLDRLVAARRDVLAIGKIEDLFAGQGITRAVHTRSDEEAMDRV
                     EEAVREGGGALVFANLVDFDAQYGHRNNVEGYAENLERFDARLGRLLPLLLPGDLLIL
                     TADHGNDPTTPSTDHSREYVPVLAVGDAVRPGTDLGTRATFADLGATVAEALGVEGTG
                     HGTSFLRELVS"
     gene            15054..16112
                     /locus_tag="PKK95_03520"
     CDS             15054..16112
                     /locus_tag="PKK95_03520"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOC17309.1"
                     /translation="MTIREELEAREREFLSPSAAFSSETRGRLRPEPEDDIRPAFQRD
                     RDRIVHCKAFRRLKHKTQVFFAPAGDHYRTRLTHTLEVSQIARTIAKALHLHEELTEA
                     IALGHDLGHTPFGHAGEKVLDQLVPGGFTHDEQSLRVVDVLERDGAGLNLTWEVRDGI
                     AHHSKGKTGRPVGEPAGHRAQTLEGQIARLSDLIAYVNHDIDDATRAGILEPGDLPGP
                     IVEVLGPSSSSRIGRMVGDVVSATLAAGLSEVRMSEAVLAATLDLRAFLFRTVYEDRR
                     SVVQLRKATGILGGLWEKVRERPGRFLDLRILERDGMDVAARDFLAGMTDRYAVTLFE
                     RLFIPRPWVEMPGDWDAE"
     gene            16214..16774
                     /locus_tag="PKK95_03525"
     CDS             16214..16774
                     /locus_tag="PKK95_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOC17310.1"
                     /translation="MFLDLSQFREPRTHVERTFEPDEGGVVHGLTPEGEREAFGVMEP
                     VRLSFDLLKDGKNYRLAGRVATRLSLDCSRCLEPFEFPVDGTFDLLYVPHAENAAPGE
                     EIEVEEDDLGTAYYRDERIDLGQLLREQFYLAIPMKPLCRESCRGLCPQCGTNLNTGT
                     CACTNSWTDPRLEGLRALANRRDGEA"
     gene            16805..16987
                     /gene="rpmF"
                     /locus_tag="PKK95_03530"
     CDS             16805..16987
                     /gene="rpmF"
                     /locus_tag="PKK95_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOC17311.1"
                     /translation="MPNPKRRHSKARGAKRRTHDALSPAALSNCPQCHEPKLSHQVCP
                     HCGYYRGRQVREVKEG"
     gene            17009..>17438
                     /locus_tag="PKK95_03535"
     CDS             17009..>17438
                     /locus_tag="PKK95_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17312.1"
                     /translation="MDIRIAVDAVGGDHGPGPIVAGAVAAARELPVSLVLAGPVAVVR
                     PLVEALPDARGLSVRYVDAGGAIAMDEPPAAVLRRRADASILAAAGEVAAGRADALYS
                     AGHTGATVVAAHSRFGMLPGVDRPALAATILSVRMWFTRDE"
BASE COUNT         2649 a         6274 c         6123 g         2392 t
ORIGIN      
        1 tcttcaacat cctgctccag gtgttcgagg acggccacct gaccgacggg ctggggaacc
       61 gggtggactt caagaacacg atcgtcatca tgacgtcgaa catcggcgcc cgccacatcc
      121 agaagaaggg cggaatgggg ttccagtcgg ctgaccgggc cgaggtgaac cgcagcctct
      181 ccgacctggt gctgggcgac gtccgccgga cgttcaaccc ggagttcctc aaccggatcg
      241 acgagatcat cgtcttcgac ccgctctccg acgatgacct ccggcggatc ctggaactgc
      301 tggtgagcca gctcaacgag cacctggcgg accgccggct caggatcacg ctgacgcccg
      361 aggcgaccga gtggatcatc gcggccacct gtcaggaccg gtcctacggc gcgcgcccgc
      421 tgcgccgggc catccagcgc tacgtcgagg atcccctgtc cgaggagctc atccggggcc
      481 gcctgcgcgg gggcgccgtg gaggtgtacc tggacggcgg catcctggcc taccggccgg
      541 tcgccgagac gccggccggc cagacgctgg cctgaaacgt ctaattgcct gtaaactaac
      601 tctttcccgg ggtagccgct gccgcctcgg gggacgcccg cccgcttcga cgggctgcaa
      661 gtgcctcgag agaccatgcg caagttccgc ctgacctcaa tcgcggtggc gctgctggtc
      721 ctgttggctg ccgccggctt cgcccacgcg gagccgccgg ccgcccagca ggcgccggcg
      781 cccctgtcgg ggaccattac gtggtgcggc gcgcaggtgg gcccgccgcg gaacctgccg
      841 cccgccgact ccggcccggt gctgtacctc ctcggcgttt gcttccccga gcagggcggc
      901 gtgtcggtca tcgagccgga aacctaccag tactacatcc agaccaagcg cagcctgccc
      961 tcccagaatg cttgggtacc gtgggaccag tcggtggaag agagcatccg cggcgacttc
     1021 aagcgcctgt gggggaccaa cttcctcgac aacctctcga tcgaggtgac cgactaccag
     1081 ttctccaacg gggtcgtcgg caagctggtc gcctacaaca tggaggagcg ccagcgggtg
     1141 aaggtcgtcg agtacatcgg ctcgaaaaag atcgagcaga cgaaggtcga cgagaagctg
     1201 aaggagagca actcggtcat tcgcctcgac tccttcctcg atcccgggct gatcaagcgc
     1261 gtctcgacga ccatccgaga gatgctcgcc gagaaggggt accagttcgc caccgtcacc
     1321 cccgagatca agccgattgg cggcggcccg aagctggtga acctcacctt ccacatcgac
     1381 gaggggccgc aggtccggat ccgcgacgtc gagttcgtcg gcaacgaggc gatccccgac
     1441 tggcggctcg cccgggagct gaagaccaac aagtccgagt ggttcctctc gttcctcacc
     1501 ggccggggca ccttccagga ggccaagtac gaggaggacg ccgagaacgt ggtcggcttc
     1561 taccgcgacc gcggctacat cgccgtgcgc gtgggcgagc ccgagctgcg cgtcctcgag
     1621 gactcgaagg accgccgcac ccggtacgtc agcctccgca tcccggtgtt cgagggggag
     1681 cgctacaagg tcggcgacgt cgccttcgac ggcaacacgg tggtgaagcc cgacgccctg
     1741 cgcaccctct tcaagctgcg ccccggcgag ttctacagcg acaaggcgat ccgcaagggg
     1801 ctcgagaagg cgcgcgaggt ctacggctcg gcggggtact tcgagttcac cggctacccc
     1861 gacctgcagc cgcgcgacga ggccgcccgg gcgcgggcag aaggggcgca ggggaacggg
     1921 ccggccgccg gggagccggc ggccgagggg gagaagccgg ccgaaggcgg agaggcggcc
     1981 gaggcgcaga agccggcgga gggcggtcag ctggccgacg ggcagaagcc ggcggaaggg
     2041 gaggcggcgg ccgccggccc caagccggag cgcgccccca gcggcgagcc gctggtggac
     2101 gtgacgatgc ggctgcagga gggcaagcag tacttcgtca accgcatcac cttcgtcggc
     2161 aacacgacca cccgcgacaa cgtcgtccgc cgcgagctgc gcctgttcga gaacggcgtg
     2221 ttcaacaccg aggcgctgaa gtactcgatc aagcggatca accagctggg gtacttcaag
     2281 gcgctcgagg gggcgccgac gtcaacgtcg agaagacgcc ggacgtcgag aaccaggtgg
     2341 acgtcacgct gaagttcgag gagcagaacc gcaaccagat caccttcggc gccgggatct
     2401 cgcagtggga ggggttcttc gggcagctcg ccttccagac ggcgaacttc atgggccgcg
     2461 gcgagaccct gacgctctcg ctgcaggccg gctcgcgcgc gcagaactat cagctcgcct
     2521 tcaccgagcc gtacctgttc gatcgcgcca tcacgggcgg gatcgatatc ttcaagcgcg
     2581 agctgcgcta catctcgcag ttcacgcagt cctcgtacgg gggcaacgtc ctcttcggct
     2641 tcccgctgcg cgacttcacc cggatgttcg tccagtacag ctacgagaac gtccgcgtga
     2701 aggacatcaa cccgatcttc accacgccgg agtacatcgc ggggaacccg tacctcgccg
     2761 actacctcct gctcggcgag gacaacaact cccggacggt gagcaagatc gtccccagcc
     2821 tggtgcacaa cacggtcgac gacccgattt tccccaaggc cgggacacgc tatactgtct
     2881 cggttgacct ggccggcctg ggcggcgaca ccaacttcta caagccgcgc gtcgaggggg
     2941 tgtggtacat cccgcacacc cggcggacgt cggtcggcct gcgcgctcag gcggagtaca
     3001 tcacccagat caaggggaac aagacgctgc cggtgttcga gcggctgtac ctgggcggcg
     3061 agtacagcgt gcgcggcttc gacatccgga cgatcggccc gaagctgcag aacggcctgg
     3121 tcatcggcgg gaacaagagc ctcctgctca acgccgagta cctgatcacg atcgccggcc
     3181 ccgtacgcct ggtgatgttc tacgacgcgg ggcaggcgct cgacgtcggg cagaagttct
     3241 cgctgaagga gttcaggacg tcgaccggcg ccgagatccg gttcttcatg ccggtgctca
     3301 acgtgccgtt ccgcctcatc ttcgcgtaca acccccagcg cgacggggtg ctggacaaca
     3361 acctccagcc ccagaaggcg ttctcgttca agttcgcggt cggctcgacg ttctagtcgc
     3421 gccgcccgcg ctgagccgtg accacgttcg ctcgagacga cgacagagaa agaaagggac
     3481 tgttccgtat gagacgcctt gccgttgccg ccgtcagctt cgccctggcc gcgctcccga
     3541 ccctggcttc ggcccagacc gccccgcagg ctcccgcgca gcagaagccc gccgaggtgc
     3601 ggcccttccc cgagggcgcc aaggtggcct tcatgaacat ccagcaggtg gcctccgatt
     3661 cggccgaggg gaaggccgcc aacgccaagg tgcaggcgct tcgcgacaag aagctgcagg
     3721 agctccaggc gaagaacaag cagctcgagg cggcgcagca gaaggtgcag ggcggcaacc
     3781 tgatgagcga gcaggctcgc gcgcaggcgc agaaggaggt cgatcggctg accgtcgaga
     3841 tccagcgcgc gcagcaggac gccgacgccg agctgaatga gctgtcccag gaactcaacc
     3901 tcgagttcca gcgcaagctg gggcccgtca tccagcaggt ctcccaggag cgcgggctgc
     3961 acatgctgct cagccgcgcg gacgccggga tcatctgggc cgagcccggc ctcgatctga
     4021 ccggtgagat catcaagcgc ttcgacgcgg ccaccgcggc cggcgccaag ccgcccgccg
     4081 ctccccccaa gcggccgtag ccgggcgcgc gcgatccggc gggggccccc tgggaccccc
     4141 gcctctctct cctcttcccc tgggctgaac gtgcgaccca ctaccatccc cgtgctgctc
     4201 gaccgcctgc gctaccgcta tccctcgcgc ctggtcgatg cggtcctcga acacgttccc
     4261 ggcgagcgcg tggtcaccgc caagaacgtc acggtcaacg agcagttctt ccagggccac
     4321 ttcccgggcg cgcccgtgct gcccggggtc ctgatgatcg aggccatcgc ccaggccgcc
     4381 gccctgctgc tgctgcacgg cgacgagcgc ctgcccaccg cgcgcgtggt cctccgcggc
     4441 gtcaacggcg cgaagttccg ccgccaggtg gtgcccggcg accgggtccg catcgaggtc
     4501 agccgccgca ccgggcgggg caccatcgct cgcgtgcacg gcgaggcgtt cgtggccagc
     4561 cagctcgtgg tcgaggccga cctcctgctg gccatcgtcc aggacgacgt gtcgatccac
     4621 ccgaccgcgg tcgtccaccc gcaggcccgc atcgggcacg gcacggtcat cgggccgcac
     4681 gccacgatcg gcccccgcgt cgtcatcggg aaggactgcc gcatcgggcc gtcctcggtg
     4741 atcgacgggt gcaccgagat cggcgacggc aaccacctcg cggccttcgt ctcgatcggc
     4801 ctgccgcccc aggacctgaa gtactccggc gaggataccc gcgtcgtaat cgggaaccgc
     4861 aacagcatcc gtgagttcgt caccatccac cgcgggacgg cgggcggcgg gggcgagacg
     4921 aggcttggcg acgacaacct gctgatggcc tacacgcacg tggcccacga ctgccaggtg
     4981 ggcagcggga tcatcttcgc gaacaacgcg accctcggcg gccacgtgca cgtcgaggac
     5041 ttcgccatca tcagcgcgct ctcgggggtt caccagttct gccgcgtcgg ccgccacgcg
     5101 ttcatcggcg cgcacaccgc ggtgaccaag gacgccctgc cgttcgtcaa gacggtcggc
     5161 aaccgggctc gagtctacgg cctcaacacc gtcgggctcc tccgccgcgg cttcactcac
     5221 gaaagcatcg gcaagctcaa gcgcgcctac cgcctcctgc tgaagctcaa caccggccgc
     5281 gccgtgatcc ggatcgaggc cgacccctcg ctccagtgcc ccgaggtcgc gtacctcgtc
     5341 gaattcatcc gcggcagctc gcaccgcggc gtcctgctgc gccgcccgac gcgccgtgcc
     5401 gacagcgagg tttccgacga gtagcccgac atgcacatcg gcctgatcgc cggaaacgga
     5461 cgcttcccct tcctcgtcct cgacgccgcg cgccgcctcg gccaccaggt gaccgtcgtc
     5521 gcggtgcgcg aggaggcctc gcccgacctc gaagcggccg ccgccaccgc cgagccccgc
     5581 gccgcgatcg tctggctgtc gctggggcag ctgggcgcct gcatcgagac gttccgcgcc
     5641 gccggcgtca cgcacgcctt gatggccggg cagatcaagc acgtcaagat cttctcgggc
     5701 atcgtgcccg atcgcctgct gctctccgcg ctgctgcgcc tccggtcgaa gaacaccgac
     5761 gccctgatct ccgccgtcgc cgagatcctc cgggagcacg gcatcgagct tctcgactcg
     5821 acggccttcc tcacgccgct gctcgcgccc tccggccggg tggccgggcg ccacccgagc
     5881 gccgacgagc gcgcagatct cgagttcggc taccggatgg ccgacgcgat cgccggcctc
     5941 gacatcggcc agacgatcgc cgtcaaggcc gccgccgtcg tcgccgtcga ggccatggag
     6001 gggaccgacg cggtcatcgc gcgcgcggga cgcctcgcgg gcccgggggt cgtcgtggtg
     6061 aaggtcgcca agccgaacca ggacatgcgg ttcgacgtgc cggtggtggg cctcgccacc
     6121 atcgaggcga tgcgggcggc gggggcctcg gtgctgtcgg tggacgcggg caagacgctg
     6181 ttgttcgacc gcgaagccct ggcggcggcc gccgcccagg ccggcatcgc ggtggtgggg
     6241 cgggaacgat gactgggaac cgagtgctgc gcgtcgcggc catcggcgtg gggcacctcg
     6301 gccgccacca cgcgcgcatc ctgggctcgc tcgaaggtgc cgagctggtt ggcgtggtgg
     6361 acgccaagcc cgatcgcgcc cgggagattg ccgcctcctg cggcacgcgc gcggtcgagc
     6421 gcgccgccga cctgctcggc caggtggatg ccgtctcgat tgcggttccg accgaggcgc
     6481 acttgcaggc ggcgatgccg ttcctcgagc gcggcatccc cgtgctcgtg gagaagccgc
     6541 tggcgcgcga cgtcgcggag gccgaccgga tgatcgacgc cgccgcccgc gccgggacga
     6601 tcctggccgt cgggcacacc gagcgccaca acccggccgt cgccctcgcg gcagcccacc
     6661 tcgccgaccc ccgcttcgtc gaggtgcacc gcctcgggac ctttcccgag cgaagcctcg
     6721 acatcgacgt cgtgttcgac ctgatgatcc acgacctcga catcgtgctc tcgctcgtcg
     6781 ccgccgaggt ggccgcggtg gaggccgtcg gggtgccggt tctcaccggt cgcgtggaca
     6841 tcgcgaacgc gaggctgcgg ttccagaacg gctgcatcgc caacatcacc gccagccgca
     6901 tcagccgcga gcgtgtccgc aagatcaggt tcttccagcc ggaggcgtac ctctcggtcg
     6961 actacgccgc gcaggaggtg gagatgtacc gcctcgtgcg gggtgacggg cggccggcca
     7021 tcgaaggtgg caagctcgag gtccagcacg aggagccgct gcgccgcgag ctttccgatt
     7081 tcctcgacgc ggtgcggcag cgccgcccgc cgctcgtcag cggctcggac ggccgccggg
     7141 ccatcgtcct ggcccaacgg attgtcgagc ggatggcaca gggcaactga ccggcaggct
     7201 gccgtgatcg acaaacgccc cgcgcgcgtc gccggcatgt tcgacgccat cgccgcgcgc
     7261 tacgacctgc tcaatcatct gctcagcgca gggctggatc gcggctggcg ccggcgcgcg
     7321 atccgctcgc tcggactgag gggcggagag acgctcgtcg acctctgcac cggaaccgcc
     7381 gacctcgccc tcgccgccgc cgcgggcggc gcgggccgcg tcatcggcgt ggatttcgcg
     7441 gcggagatgc tgcggctcgg ggcggccaag gtgcggcgcc gcggcggcgt tgccctggtt
     7501 cgcggcgacg ccacgtccct gccgatcggt tcgggcacgg ccgatgccgc cacggtcgcc
     7561 ttcggcatcc ggaacgtcga gcgccccgag cgggcgcttg ccgagatgtt ccgcgtcctc
     7621 cggccaggcg gacgcgccgc catcctcgag ttcagcatcc cggcctcgcc cgtcgtgcgc
     7681 gccgtctacc tgccctactt ccggcacgtg cttccgcgaa tcgggcggct cgtctcgggg
     7741 cacctcagcg cctacagcta cctgcccgcc tcggtcgggt cgttcatcgc gccgtccgac
     7801 atggccgcgc tccttcgccg cagcgggttc gaccaggccg aggccgcgag cctcgcgctc
     7861 ggcgccgtga cgctttacac cgcccgcaag gccgccgccg actggtagac ggcatcgagc
     7921 tcagcgacca tcgacgagag ccggaacagc gaagacgcgc gctgccgggc ggccgccccc
     7981 atccgcgagc gaaggccggg gtcttggcgc agacggtcga tcgcctcggc gagcctggcc
     8041 gggtcgcacg ccgggacgac caggcccgtg gtccccgact cgttcaccca cgatgtcccg
     8101 ctggccaact cggtgctgat gaccggcagt ccggccgcca tggcctccac catcgaggtg
     8161 ccgaatgcct cggcccggct ggtcgagggc agcacgaaca ggtcggccgc ccggtagcac
     8221 cccggcaggt cccgctcgct cacctccccg gcgaaggtca ctctctccga cacgtccagg
     8281 gccgccgcca ggcgctgcag gcggcgtttg agcggcccgt ctcccacgag cagcaggcgg
     8341 gcggccggca cccgcgcaat cgcgcgcagg aggtgatcga ggcccttgta gtgccgcatc
     8401 cggccaacga acccgacgac gaacgcctcc tcggcaatgc cccagccgcg gcgcaggcgc
     8461 tgcccgctcg cgcggccgtc ggccgcgaac cgctcgacgt cgatgccgag cggcaccacc
     8521 gagcacttgt cgctgatgga aggcaggtac cgcgaggtgc gcaggtatgg cggactggtc
     8581 accaccactc gatcggcgcg ctccaacacc cggcgcagca gcggctcgta gagccggagc
     8641 agccgccgct gcctgacgat gtcgcagtga taggtgatca cggttggcgt gccgcggcca
     8701 agcaccaggc gcgccagctc gccgggggga tacgggaaat gcaggtggat caggtcgggc
     8761 cgctcgcggc gcaggcgaga gggaagggtg gggcagatcg gggtcgagca cacggtcgca
     8821 agccgcggca gaagaaccag ccggaccccg tcggccatcc gttccccgcc ccggtggccc
     8881 gggctggcgg ccatcaccgt gacctcgtac ccgcgacgag cctgctcctc ggccagcgcc
     8941 cggacgtggt tctcgatccc tccgatgatg gggtagtagt gcttgtagac gtgaacgacg
     9001 cgcatcggcg ccgagatcat atcagccgaa gctggctgcg gggcagtagg gccggcctcc
     9061 gtgcctgttg gctgccgggc atcagcggca agcacagggc ttcagccctg tgccactctc
     9121 tcatagaccg ccgccatctc gcgggccgcg agatcccagt cgaagcaggc ggcgcgagcc
     9181 agggccaggc cgccgagtcg ccgggcctcg tcttcgtcgg agagcagccg gaggatcgcc
     9241 gccgccaggg cctcggcgtt gccggtgggg aagagcgctg cagcgccgcc ggccgtctcg
     9301 gccagcgccg gcgtatcgct gctcacgcag gggcggccgc gggccatcgc ctcggccaat
     9361 ggcaggccga aaccttcgcg cagggacggg cagacgaaca gcagggccgc cgcgtacagc
     9421 tcgttcagct gacggtcgcc gaccggcccc aggtcggccg cgcgcgtcga gaggccgagg
     9481 cgctcgatgg tgcgcgcggg ctcggggtag cgcgggtcgc gcggcccggc catcaccagc
     9541 gtgatccccg cggtctgcgc cggggcctcg gcgagcgcga gggcccaggc acgcagcaac
     9601 acgtcgagcc ccttgtgcgg cttgttgctg ccgacctgta acaggaaccg gggcggcagg
     9661 accgagcgag ggcatcccca ctgcgccgca ggcaatgccc cggaagcggg agccggacga
     9721 tccgccgcct gcccgggcgg ggcctgtacc caggtcacgc cggggcggac gatggtcagc
     9781 ttgccgcggg cggccgggaa caaacgctct acatcgcgac gggtcgcctc ggagggtacg
     9841 cagacgatgg cggacgcgcg gaaggcgaga cggtgagcca gcacgtaacc gaagcgccgg
     9901 ccgggcccga ggacgccggg ggtgagcatc gggatgaggt cgaagcaggt caccaccgtt
     9961 gggatgccag gccggtaggg catcaggtag tacgggctgt ggtagacgcg cgcgcccgct
    10021 ttccgcagca ggcgtgggac ctcccactgc tgccgcaggg cgaacgggcc ggcgccgcag
    10081 ggcacgccgg cgagcggcgg gaccggcagc cgcgggtctg ggtgcgcggc gtgcagcagt
    10141 gtggccgaac cgaggcgtga gagtgccccg gcaaggccaa ggaccactcg gccgatcccg
    10201 gggaagtggc cggagacggt gcgcgcgtcg aggaccagcc cctccgggat cacgacacgc
    10261 cccccgcacc ctccgtcccg tctttcgcct tccacccccc gctgtccgtt ccgccctctg
    10321 tcctcggtcc cccgcgttcc ttcccggcca gctccctgta gaacgccgcc gtctgccgtg
    10381 cgagagtggc ctccgagaac tccgcgaccg cgcgctcgcg tccgcgccgg cccagctgcc
    10441 gtcgcaaggc cgggctctcg cgcagcgtcc gcaggcgctg ggcgagagcg gccgcgtcgc
    10501 cctcggggaa gacgaggcct gcgtccccga tcacgtcggg gatcgcgccg gaatcggagc
    10561 cgaccacgct gaccgcgcag gccatggcct cggcgagcac acgcccgaac tgctccttcc
    10621 acacggccgt ggtccgcgag ggaagaacga cgacgtcgaa cccggcgaat acccccggca
    10681 aagactcgtg cgacagcgtg ccggtgaagc ggacgcggcc ggcgagcccg agggcgccgg
    10741 cacggacctc gagcgcccgg cgctcggggc cgtcgccaac cagcgtcagc tcggcaggcg
    10801 gttcgagcgc ggcgacggcg tcgagcaggg tggcacaccc cttctcggcg gccagccgcc
    10861 cgacgtaccc cacagagaac cggccggggg gcgggggcgc agggggcgga gcgaagctgg
    10921 agacgtcgac gccctggggg aggatcgtcc tcgtcaggcc ggtgaacccc agcgatcgcc
    10981 acactcgctc ggcctcgcgg ctggcacaga gaatggcgtc ggccgacctc agcgtcaggg
    11041 cgagcagcgc gcgggcacca gggcccagct ctcggcggac gttctgccag gtgtggtaga
    11101 cgagccgggt gcgcctggcc cagcgcctgc gggccagggc cacctgcagg gcagcgaggc
    11161 tgtcgggctc ctcctcgacg tgcacgatgt cggggtggaa ccggcgcagg ccgaaggcga
    11221 gggtgccgta caggcagcgg tgggggtcgg cacgcctcca gaccgggacc cggagtgtgc
    11281 cgtccaaacg atcgggggtg cgggtggaac ggacgaggtg aattcgaagg ccagcgtcgt
    11341 ctctcaagcg ctcgatcttg ccctccgaca cctggtgcga gtgggcgacg tggaggacgg
    11401 ccagcatcgg ccgcccacca taccacagcc gatggtataa tcccctatct tcttcatgta
    11461 cttcgacttc gaggacaatc acccggacat ctcgccaatc ggacgcgcca tttcctggcg
    11521 cgagggcgtc ctcctgtcga tcatcttcca cctcgtggtc attctcgcgg ccgtgctgat
    11581 gcccgagttc cccggcgcca gggaagcggc ggcgcgggcg gccgaagcgg cggagctgca
    11641 gaggcagcag gagctggagc ggacgcggtt cgttttcgtg cagccgcgcc tcgacgtccc
    11701 cgcgcccaag ccgcccccgc cgagagccga tctgtcggac aaggaccggg tggcccaggc
    11761 gccggaacgc gcgcaaaacc ccaccaacag cctgccgttc tcccgcggca acacgcccga
    11821 gcaggtggac cagccggcgg tgatggcgcg ggaagcgccg cgcgtcgagt cgcgtcccga
    11881 gccgggtcca agcgaggagc agcgggcgca ggccgatcag cgccaggggc agggcggcga
    11941 gaacggcacc ggggaagcga accggagcgc gctgtcgctg ccgggccgaa ggcagcctgc
    12001 gtccaggccc ggggagggaa ccggccgtcc gggtgcgccg gggctgttcg ccggcgcgat
    12061 gcagaacccc gagcgctatg cgagccagcg cttccagaac gaggggggtg ggggcggcgc
    12121 cttcgggccc gcgatccagt tcgacaccaa gggcgtcgag ttcggcccct gggtgcggcg
    12181 cttcatcgcc cagatcaagc gcaactgggt cgtcccttac gcggccatgg ctttcagcgg
    12241 ccacgtggtg ctgaccttca acgtgcacaa gagcggtgcg attaccgacc tgatggtggt
    12301 ggggccgtgc gccgtggagg cgttcaacaa ctcgtcgttc ggcgcgctgg ccgcctcgaa
    12361 cccgacctat ccgctgcccc ccgagtaccc gtccgaccgc gcgttcttca ccgtgacgtt
    12421 ctactacaac gaggttcccc cggttcagta ggggacgatt cccccgatgc caacccgcac
    12481 gcagcaagtc ggcttgttga tcctggccgc cgtgctcgcc ctttacacca tggcgcggct
    12541 gctgtggtaa gcgagcggcc tcccgtgctc gtggcggttc tcgggccgac cgggagcggc
    12601 aagagtgcgc tcggcctggc gctcgccgaa cggttcggcg gcgagatcgt cagctgcgac
    12661 tcggcagccg tctatcgcgg cctcgacatc ggcaccgaca agctgccgct cgaggcgcgg
    12721 cgcgggatcc cgcaccacgt ggtggacgtg gccgacccgc gcgagtcgta cacggccgcg
    12781 cggttcgcgc gcgacgcgtc cgaggcggtg cgcgcgatcc atgcgcgggg gcgcctgccg
    12841 atcctggtgg gcggcaccgg cttctactac cgggccctga cgcgcggtct gttccccggg
    12901 ccgtcgcgcg acgaggcgct tcgcgagcgc ctgcgcgcgg cggcgcggcg ccgcggggcc
    12961 gggttcctcc accggatggt ggcgcgggtc gatcccgggt cggccaggcg catcctgccg
    13021 cgcgacgagg tgcggttggt gcgcgcgctg gaggtgtact tcctcaccgg gcggccgctc
    13081 accgcgcact tcgccgacac ggtgtcgccg ctgcccggcg tggaggtggt gggcctgctg
    13141 ctgcaggtgc ccgctgccgt gctcggcgag cgcatcgccg cgcgcgtcgc cggccagttc
    13201 gcccgcggat gggtgaacga ggttcgacgc ctgctcgcct ccggtgtgcc gccagacgcg
    13261 cacgcgttcc aggcgctcgg ctaccgcgag ctgatcgagc acctggaggg ccggctcgac
    13321 gagcgcgcgg cgcgcgagct gatcgtcgcc gagacgcgga agtacgcgcg gcgtcagttg
    13381 atctggttcc gtaaagagcc taatctagag gtgattccgg gccctggaga acgtggcact
    13441 gccatcgccg ctgcgacgcg cctcgtcgca cgccggctgc caggcagccc ctgcgcgcgc
    13501 gacgaggaga ccccatagat ggcttccgca accgagcccc ggcccgtggt caccagcccc
    13561 aacatccagg acgcgttcct caaccacgcg cggcgtgagc gcctgaccgt gcggttccgc
    13621 ctgctcgacg gcaccgagtt cgagggccga atcaagaact tcgaccgctt cgcgatcatc
    13681 gtggaccgcg agggcgccga ccagatgatc ttcaagcacg cggtcgccac cattacctct
    13741 tcgcgcaccg tcggcagcta ctacacgcac cagtcgcagt gacccccccg cccatccgcc
    13801 gcgcgatcgt catcgttctc gacagcctcg ggatcggaga gctccccgac gcggcggcat
    13861 acggcgacca gggcagcgac acgctcggcc acatcgccgc gtgcgtgccg ctcgcggtgc
    13921 ccgcgctgcg ctcgctgggg ctcgggcggt tgtcgaacct cggaccacag gcgaggacgc
    13981 ctgtgccact ggcaccacag gcgaggacgc ctgtgccact gggggccttc ggccggatgg
    14041 tggagagcgc gccgggcaag gactcggtga cgggccactg ggagctgatg gggctggtgc
    14101 tcgaccggcc cttcccggtt ttcccgcacg ggttcccggc cgaccggctc gaggagttcg
    14161 agcgccgcat cggccggcgc accctgggca acacggtggc ctcgggcacc gtgatcatcg
    14221 aggagctggg gcccgagcac ctgcgtaccg cttcgccgat cgtctacacg tcggccgaca
    14281 gcgtcttcca gatcgcggcg cacgaggacg tcatcccgat cgacgaactg taccggcagt
    14341 gcgagatcgc ctacgcgatg ttcgtcgagg ggatgggggt ggggcgcgtc atcgcgcggc
    14401 cgttcgtcgg ccagcccggc tcgttccgcc gcaccgcgaa ccgccgcgac ttcgccatca
    14461 ggcccagcgg cccgaccgtg ctcgaccgcc tcgtcgccgc ccgccgcgac gtgctcgcca
    14521 tcgggaagat cgaggacctg ttcgccgggc agggaatcac gcgcgcggtc cacacgcggt
    14581 cggacgagga ggcgatggac cgggtggaag aggcggtgcg ggagggcggc ggggcgctcg
    14641 tcttcgcgaa cctcgtggac ttcgacgcgc agtacggcca ccgcaacaac gtggaggggt
    14701 acgcggagaa cctcgagcgg ttcgatgccc ggctcggccg gctgcttccg ctgctgctgc
    14761 ccggcgacct tctcatcctg accgccgacc acggcaacga cccgacgact cccagcacgg
    14821 accattcgcg ggagtacgtc ccggtgctcg cggtcggcga cgccgtgagg ccggggacgg
    14881 acctcgggac ccgcgccacc tttgccgacc tgggcgcgac cgttgccgag gccctggggg
    14941 tggagggcac gggacacggt acgagctttc tccgggaact cgtctcctga tcggccgccg
    15001 ccgctgccgc gctccggcgg cagccggccg ggagaggcaa gcgagcgacc cagatgacca
    15061 tccgcgaaga actcgaggcg cgcgagcggg agttcctctc gccctccgcc gccttcagct
    15121 ccgagacgcg cggccggctg cggcccgaac ccgaggacga catccgcccc gcgttccagc
    15181 gcgaccgcga ccggatcgtc cactgcaagg cgttccggcg cctgaaacac aagacccagg
    15241 tcttcttcgc gccggccggc gaccactacc gcacgcgcct gacgcacacg ctcgaggtct
    15301 cgcagatcgc ccggacgatc gccaaggcgc tccacctgca cgaggagctg accgaggcaa
    15361 tcgctttggg gcacgatctt ggccacactc ccttcggaca tgccggcgag aaggtgctcg
    15421 accagctcgt gccgggcggg ttcacccacg acgagcagag cctccgcgtg gtggacgtgc
    15481 tcgagcggga cggcgccggg ctgaacctca cctgggaggt gcgcgacggg atcgcgcacc
    15541 actcgaaggg gaagacggga aggcccgtcg gcgagccggc cgggcaccgc gcccagacgc
    15601 tggagggcca gatcgcccgc ctctcggacc tcatcgccta cgtcaaccac gacatcgacg
    15661 acgccacgcg cgccgggatc ctggagccgg gcgatctgcc cgggccgatc gtcgaggtgc
    15721 tcggtccctc ctcgtcgtcg cgcatcgggc ggatggtggg cgacgtggtc tccgccacgc
    15781 tggccgccgg gctgtcggag gtccgcatga gcgaggcggt gcttgcggcc accctcgatc
    15841 tgcgcgcatt cctcttccgc acggtctacg aggaccggcg atcggtcgtg cagctgcgca
    15901 aggcgaccgg gatcctgggc gggttgtggg agaaggtgcg cgagcggccc ggccgcttcc
    15961 tcgacctgcg gatcctcgaa cgcgacggca tggacgtggc ggctcgcgac ttcctggccg
    16021 gcatgaccga ccgctacgcg gtgacgctgt tcgagcggct gttcatcccg cgcccctggg
    16081 tggagatgcc cggcgactgg gacgcggagt gaaacgcgcc gcgtccccgc gcgtcgcggc
    16141 cgggccggcc caggctgccg tggtataatc atgggtccgt cccgcgacgg ccgaggcggc
    16201 agggaagacc ccgatgttcc tggacctgag ccagttccgc gaaccgcgca cgcacgtcga
    16261 gcgcaccttc gagcccgatg aaggcggggt cgtccacggc ctcacgcccg agggcgagcg
    16321 ggaggcgttc ggtgtgatgg agccggtgcg gctgtcgttc gacctgctca aggacggcaa
    16381 gaactaccgg ctcgcgggca gggtcgccac gcggctgtcg ctcgactgca gccggtgcct
    16441 cgagccgttc gagttcccgg tggacggaac cttcgacctg ctctacgtgc cgcacgccga
    16501 gaacgcggcg cccggcgagg agatcgaggt cgaggaggac gatctcggca cggcctacta
    16561 ccgggacgag cggatcgacc tggggcagct gctgcgcgag cagttctacc tggcaatccc
    16621 gatgaagccg ctgtgccgcg agtcctgccg cggcctctgc ccccagtgcg ggacgaacct
    16681 caacacgggc acctgcgcgt gcacgaactc gtggaccgac ccgcggctcg aggggctgcg
    16741 ggcgctggcg aaccggcgtg acggcgaggc ctgagcccgg ccagcagata gacaggcgga
    16801 tagcatgccc aacccaaaac gacgacactc gaaggcccgg ggcgccaagc ggcgcacgca
    16861 cgacgcactg tcaccggccg ccttgagcaa ctgcccccag tgccacgagc ccaagctctc
    16921 gcaccaggtg tgcccgcact gcggctacta ccgcggccgg caggtgcgcg aggtgaagga
    16981 gggctagggc gcgccgcatc ggttcaccgt ggacatccgc atagccgttg acgccgtcgg
    17041 cggcgatcac gggccggggc cgatcgtggc cggggcggtg gccgcggcgc gcgagcttcc
    17101 cgtgtcgctg gtcctggccg ggcccgtggc cgtcgtgcgc ccgctcgtcg aggcgctgcc
    17161 cgacgcccgc ggcctgagcg tccgctacgt ggacgccggg ggagccatcg ccatggacga
    17221 gccgccggcc gccgtgctca ggcggcgagc cgacgcctcg atcctcgccg cggccggcga
    17281 ggtggcggcg ggacgcgccg acgcgctcta cagcgccggc cacacgggag cgacggtggt
    17341 ggcggcccac tcgcggttcg ggatgctgcc gggcgtcgat cgccccgcgc tcgccgcgac
    17401 gatcctctcg gtccgcatgt ggtttacgcg cgacgagc
//
LOCUS       DAOPQX010000037        16913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4050_length_16913_cov_5.580733, whole genome shotgun sequence.
ACCESSION   DAOPQX010000037 DAOPQX010000000
VERSION     DAOPQX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4050_length_16913_cov_5.580733"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKK95_03540"
     CDS             <1..485
                     /locus_tag="PKK95_03540"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC17313.1"
                     /translation="AGQPNELRKQAAFWLAAARGREGFETIEALLPDADPAFRAELTF
                     PIHVSKEADAGEILVRMARGDESPKVRRQALFWLGQKAGAKALATIEGAIENDPDTEV
                     KKRAVFALSQMPKDEGVPRLMEIARSHRNLEVRRQAMFWLGQSNDPRALAFFEEILKR
                     "
     gene            complement(580..1857)
                     /locus_tag="PKK95_03545"
     CDS             complement(580..1857)
                     /locus_tag="PKK95_03545"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOC17314.1"
                     /translation="MARHLIAVAIATLVVTFAGAAAQQASSEAELLAKARAIHDRVFT
                     VDSHVDILGATYATPALDPGKPTRLKCDLPKMKAGGMDGVFLAVYTGQRQDFTPAGYK
                     AVYAEAIDRFDAIDRLTAMHPGLCELARTPGDVERIEKTGKRVILVGVENGYPMGEDV
                     ATLKTFYDRGARYVTLVHGSHNQLADSSGDPKPRHDGLSALGKKAVAEMNRLGIIVDT
                     SHASAKVFWDVIAMSKAPIMASHSGCTAVAKSDRNLDDDQLRALAKNGGVIQIVALGS
                     YLKEDSPERAAAVRKLREELGRGRGGRGGRGAGGPPAAPPTPQEQAEREKMFEVYRQR
                     MEEIDRQFPGATLEDFVNHIDHAVKVAGIDHVGIGTDFDGGGGVKGFNDHSEALNVTV
                     ELVRRGYTEEQIRKIWGGNLMRVWREVNRVAGK"
     gene            complement(1986..3038)
                     /gene="ltaE"
                     /locus_tag="PKK95_03550"
     CDS             complement(1986..3038)
                     /gene="ltaE"
                     /locus_tag="PKK95_03550"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOC17315.1"
                     /translation="MTIDLRSDTVTLPSAAMRQAMAEAEVGDDVYGEDPTVGRLERLA
                     AEMTGMDAALLVASGTMGNLASLLAHCERGREVILGDESHIFHYENGSASALGGLVLH
                     TVPTRPDGTLPLEAVEAAVRLPAHHYHYYHYAPTGVICLENTHNRCGGRVVSPEYFAG
                     VAAIARRHGVAVHVDGARLFNAALAAGRPVTDWTRHADSVQLCLSKGLAAPVGSLICG
                     SSAFVDRARRMRKMLGGGMRQAGVIAAPGIVALTGMVSRLAEDHANAKILADGLARIP
                     GITLDPAEVETNIVVFRLPGVEAASAYAAALEREGVLVSDFGGGRLRVVTHYGIDEAA
                     CRRAVEICARVAAAQL"
     gene            complement(3045..5135)
                     /locus_tag="PKK95_03555"
     CDS             complement(3045..5135)
                     /locus_tag="PKK95_03555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC17316.1"
                     /translation="MRSTCRLAVAAVLLAVGSSPAAPQSLPRFTVEEMLKLDRVSDPQ
                     LSPDGRRVAYVVTEVSLERNSRNSDIWMLPVPGGKPVAIARSPRSDDTPRWSPDGRTL
                     AFVSTRDGSSQVWLVGVGEAGPVGEPRKLTSLATEASGVTWSPDGKWILFVSDVYPEC
                     GSLACNEQKLKEAEGRASKARVIDGLMFRHWTSWKDGRFSHLFLVPADGSAAPRDLTP
                     GPADVPPFSLGGPTDYAFSPDSRELAFVRKTDKVEAISTNSDLFLLDLADPKAEARRI
                     TTNPAADGGPLYSPDGRFIAYRAQHRAGFEADRWQLMLYDRKAGEHRSTTVGWDRSPD
                     SYVWAPDSRSIYLTAENEGRSDVFRLRLDGGDPVPLIASGTNGDLQLSRDGRTLVFSR
                     NTLTSPADLYVADLAAAPANGQVPAKPLTNANPGLPAFKLREGESATFEGAGGARVQA
                     WIVKPPDFADGRKYPLLYLVHGGPQSAWHDGWTFRWNAQVFASAGYVVVMPNPRGSTG
                     FGQQFVDEISGDWGGKVYDDLMKGLDFAEALPYVERGRVGAAGASFGGYMMNWFLGHT
                     TRFRAIVSHASVYNLTSMWGVTEELWFPEWDIKGTPWTNPEGYAKFSPHTYAKNFKTP
                     TLVTHGELDFRVPIGEGLQLFTTLQRLEVPSRLVYFPDEGHWINQPANAALWYREFIE
                     WMNKWVPESRIPKP"
     gene            5379..7877
                     /locus_tag="PKK95_03560"
     CDS             5379..7877
                     /locus_tag="PKK95_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC17317.1"
                     /translation="MLQQSRSAIGQFVSVRQDHWRVLDVVRHDGCESWRLEGVRPSNR
                     GERPTLLVPFDRPRPLPARDLRGRAVGARRGLLALRALAAVSTSVGQLRAAAAATIEI
                     LDYQLEPVLACLQGASRLLLADEVGLGKTVQAALVLAELSSRGEAGRVLVLTPAALAA
                     QWAEELSQRFQLEPTIVERSTLARLASWSSDEEGPWARLPLAIASIDFVKQPEVLRGM
                     GGVRWDLLVVDEAHACVQAPYRAAALGWLASRARRLVLLTATPHPGDEAAFQALCHVG
                     RLAGDPPIAMFRRTRAGLGGPPTRRVRVVRVRIGEAEARMHRALLSYARRVWHRGGAS
                     SPEPAPARLAMTVLCKRAASGPGALLVSLRRRLELLEAPSPTAVQLSLPLAEEAGHED
                     EAPGDVLGAPGLEDQEEEQRELRHLIEMAREASAHDAKSRALLRLLIRARQPAVVFTE
                     YRDTLEGLAASLAPLGAAATLHGGLDGAARRAALARFASGGARLLLATDAASLGLNLQ
                     ATCRLVVNHDLPWSPMRLEQRIGRLDRIGQRRMVHAIHLAARGTFEEEVLARLALRVE
                     KVRSSIGLGASPLGTADEGRVAGTIIGGDAEPVAQAASRWHEAGSRAAFVKPDLGAEA
                     RAEAGRLRFVRALTAPAPGRPAPDITSILDSCERGAPWRFVLRADRSALAPGMYALYR
                     VRVIDGRGALLHDEVVPLFLPLPLIGRGPASRDVSWRSMAPAFGTIAGTLGATVLQKV
                     AAQLQPAIRAQRTREFQMAALEGRAPVPVQAGLFDRRAEKAAADADARRRAARFEMSA
                     RLRSLDAACRPVLASPPQPLLLFLVARGRRPRWS"
     gene            7884..11489
                     /locus_tag="PKK95_03565"
     CDS             7884..11489
                     /locus_tag="PKK95_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOC17318.1"
                     /translation="MPAGVSGSLVSDYYLDEVLRREFAGRLGEAGSDAAHAALRRWWR
                     WTGRQLGPVTPLRGVFDGAAVPLARTLGFRLSSPRPCPGAGALAAEMFARDGRSAHAW
                     PLMLVAGWGESLSSAWRYTVRETLAAGTSWCLSTNGRQLRLTDTLRSCSRRFLEFDLE
                     TVAEDRAAFVPFWALLRAEAFKPPAPAGGPKDCGEAGSLIDRIVRASAGQTIGVCRSL
                     RAGVLEAVGGMMSGLLPRRALSGAVASSDLAQLYEQALTIVYRVLFLLFAESRGLVPS
                     WHPTYRRSYSMEAARAVAESHGPARGLWETLQAMSRLAHAGCRAGTLRVTPFNGRLFA
                     PSLTPAGESARPGDECARRMLLSLTTVPGARGAPRARIVYRDLGVEQLGSVYEAVLDY
                     RPVLRPASSVAQASPPVAPVAQASPPVVPVAQASPRVVPVAQASPPVVPVAQASPPVV
                     HLVPGSGLRKATGTFYTPSPITEFIVRRALWPLARDAAPEQILGLRVLDPSMGSGAFL
                     VAACRYLAAAYEEALVRTGRYHPSDIGDAERRTFRRLVAQHCLYGVDVNPTAVQLARL
                     SLWLATLAPDRPLSFLDHRLLVGDSLVGASLDDVARRPFPGGRLRGPAAGTGTLPLFG
                     DGDAEAAVRTVLPSRLKLASEPDDSVQAVREKEQVLASMEGPASPLARWKLVADAWCA
                     LAFGLDSGERPPPSLFTALADRLAGRASPLPVLEADRWMAEVSRTARAHRFFHYTLEF
                     PEVFFDESGQPRADAGFDAVLGNPPWDMIRDDSGATGAAPLHAGDAAGDAGGQRAGRN
                     SPAARLARFARASGVYHLLSDGHRNRFQLFVERAVCLARRGGRVGLVVPSGLLTDCGC
                     APLRRLLVDRCEVDGMVCFDNAGRIFPIHRSVRFVAFTATSGVPAGRLPCRVGERDTS
                     VLDRIPDHAGAEHFPIVFTRGFLERVSGADLAVPDVRSAADLALLDRLVAAAPALGSP
                     EGWNARFGRELNATDDNSLFWKGASGMPIVEGKHVHPFRLELPASLTRLRPEAAAALG
                     ARGAWFARPRLAYRDVACATNRLTSIAAIVPAGYATVHTVFCLKTRLAEADQWFLCAM
                     LNSLVVNFLVRLRVATHVTAGIVARLPVPRPPSHSPGFRRIAWMARRLGGSTHPERDR
                     RYALLQAEVARLYGLTEEQLEHVLAGFPLIEDETKRQVRAAWRPPSHQALRRGKP"
     gene            11521..12351
                     /locus_tag="PKK95_03570"
     CDS             11521..12351
                     /locus_tag="PKK95_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOC17319.1"
                     /translation="MPAPSMALTSILFLGGIAAGGLGSLLGLGGGVFLVPLLVLVMDV
                     PMRVAVGISLTTIIATSSVTTSGSVGRSLINLRLGMLLEVATTVGGLTGGLVAVLISQ
                     RRLQQLFGVVAAAGAAIMLSRLGKRNVLLDPAAEPGRLGGRYYEEESRGVVTYQVKRL
                     PLALFVSLAAGSVSSLLGIGGGMLKVPALNAWCGVPLRAAAATSAFMIGVTATVGAVI
                     YYGRGEIVPWMAAAAILGVMVGSRAGFRIGAAASARWLKLLLAIVLVIVSILMFLRAR
                     "
     gene            12348..12668
                     /locus_tag="PKK95_03575"
     CDS             12348..12668
                     /locus_tag="PKK95_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HOC17320.1"
                     /translation="MSAGEGRELRANADDRLERWLGKTLGAGTMLATALLALGLLLQF
                     AGVGAEVSDRLSRAGLIVLMATPVARVLISVAEYAGQRDWPFLALTLTVLGMLMVSFL
                     LGVG"
     gene            12725..13666
                     /locus_tag="PKK95_03580"
     CDS             12725..13666
                     /locus_tag="PKK95_03580"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOC17321.1"
                     /translation="MRIAVLGAGAVGGFYGARLARAGHQVSFVARGRSLEALRSSGMS
                     VRSDLGDFVVYPAAQSDPVRIGTVELAIVAVKTYSNGEALPLLKPLVGPGTMVLTLQN
                     GLDSAAEVAAVVGEGHVLAGTTYIGVSLEAPGEVLHVGTARRIVFGEAFGPARVTERV
                     ARLEEVLRGADIHAEAVADSRTALWEKLVFLAPIAALTAAARLPIGPLRSSPEFREAA
                     LRAMREVESVARAEGVGLPADVCEQKMAYTDATSPAMRSSLMMDVVAGRQTEVEALLG
                     GVVRRARQRGVPVPAMQTLYAVLAHSANAVALPGGAS"
     gene            13739..15463
                     /locus_tag="PKK95_03585"
     CDS             13739..15463
                     /locus_tag="PKK95_03585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOC17322.1"
                     /translation="MRSRLATLIVVAIVAGTFVIGLIVGAQRDDETGPVDLIVLNGRI
                     YTADGSGRFAEALAVRGNTILRVGSNREVKRLAGRSTETIDAHGASVLPGLNDAHLHL
                     VSGGLGLQQANLLDASTLEEIGAAIEAFAAAHPDRPWVLGRGWYYTPFPGGLPTRQQL
                     DALVPDRPAYMRCYDGHTAWVNTLALKAAGITRETPDPPGGVVVRDPATGDPTGVLKE
                     AAQELVARHLPEWTRDDRLRAIGDAIAKAHELGVTSAQNAGVDEAEVELYDELLKKGD
                     LQLRLYNAISIEPPFTAADADRLDGLWKRRPDDALVKMGAVKLMLDGVIESHTAAMLE
                     PYANRPATGLPNMDAATLNGIVAMMDRRGWQVLVHAIGDAAVRQSLDAFEHAAAVNPV
                     PARGRRHRIEHVETLQPSDVPRFGRLGVVAVQQPFHGNPNPTQLAVWSANLGPERASR
                     GWAQRSILDAGGRLAMGTDWPVVTLDPRYQINMAINRTTPEGTPPGGWLPEQRLTLAS
                     AVDAYTTGAAYASFDERRKGQLVPGMLADIVVLSTDVFANAPEKFLDAEVAVTIFDGK
                     VVYRKTQP"
     gene            15460..16893
                     /locus_tag="PKK95_03590"
     CDS             15460..16893
                     /locus_tag="PKK95_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOC17323.1"
                     /translation="MNAKPPKRPVLLAGSWEESATPLDVVNPYDGTVVASTFAAGEAE
                     LERATQAAVAAAPGMRRLAAYERAEILARAGDELRRRREEIGRTIAAEVGKAIRDALA
                     EADRGVQTFQAAAEEARRIAGEEIPIDLAPHGKGRLAFTRRFPIGPVAAISPFNFPFN
                     LAAHKLAPSIAAGNPVVLKPASKTPLSALILAEALDRAGVPKGALSVLPMSRATGERM
                     VTDDRFKLLTFTGSSSVGWGMKARAGRKRVLLELGGNAGVVVDETADVAHAVARIAAG
                     GFALAGQSCISVQRVYVHRRLYERFAAMLVEKVEALKVGDPLDPATDLGPMIDEDEAG
                     RVDAWIREAVREGARVLTGGRRLGKALYAPTVLVDVPPDAKVCAEEVFAPLVGLFPFD
                     DFDRAVEEVNRSPYGLQAGVFTNDLRNALAAFDRLEVGGVMVNDVSSWRMDAMPYGGV
                     KNSGLGREGPRYAIEEMTETRIVVFAP"
BASE COUNT         2255 a         6040 c         6033 g         2585 t
ORIGIN      
        1 ccgcgggcca gcccaacgag ctgcgcaagc aggcggcgtt ctggctcgcc gccgcccgcg
       61 gccgcgaggg gttcgagacc atcgaggcgc tgcttcccga tgcggacccg gcgttccggg
      121 ccgagctgac gttccccatt cacgtgagca aggaagccga cgccggcgag atcctcgtcc
      181 gcatggctcg cggcgacgag agcccgaagg tgcgccggca ggcgctgttc tggctgggtc
      241 agaaggcggg cgcgaaggcc ctggccacca tcgagggagc gatcgagaac gacccggaca
      301 ccgaggtgaa gaagcgggcg gtgttcgccc tcagccagat gccgaaggac gaaggggtgc
      361 cgcgcctgat ggagatcgcg cggtcgcacc ggaacctcga ggtccggcgg caggcgatgt
      421 tctggctggg acagtcgaac gacccgcgcg ccctcgcgtt cttcgaggaa atattgaagc
      481 gctgagcctc gcgtgacgcc ggttcgttgt ggtcaggggc ggccacaagg gccgccccgc
      541 gtgctcacgt agggcgacca caagggtcgc ccctacggct cacttccccg ccacccggtt
      601 cacttctctc cacacgcgca tcaggttgcc gccccagatc ttgcggatct gctcctcggt
      661 atacccgcga cgcaccagct ccacggtgac gttcagcgcc tcggagtgat cgttgaaccc
      721 cttcaccccg ccgccgccat cgaagtcggt cccgatgccc acgtgatcga tgcccgccac
      781 cttcaccgcg tggtcgatgt gattgacgaa gtcctcgagc gtggcgccgg ggaactgccg
      841 gtcgatctcc tccatgcgct gcctgtagac ctcgaacatc ttctcgcgct cggcctgctc
      901 ctgcggcgtc ggcggcgcgg ccggcggccc gccggcgccc cggccgccgc gcccgccccg
      961 cccgcggccc agctcctcgc gcagcttccg cacggcggcg gcgcgctcgg gcgagtcttc
     1021 cttgaggtac gagccgagcg ccacgatctg gatgacgccc ccgttcttcg ccagcgcgcg
     1081 cagctggtcg tcgtcgaggt tgcggtcgct cttcgccacc gccgtgcacc cggagtgcga
     1141 ggccatgatg ggggccttcg acatggcgat gacgtcccag aacaccttgg ccgacgcgtg
     1201 cgaggtgtcc acgatgatgc ccaggcggtt catctcggcc acggccttct tccccagcgc
     1261 cgagagtccg tcgtgacgcg gcttgggatc gcccgacgaa tcggccagct ggttgtgcga
     1321 cccgtgcacc agcgtgacgt agcgggcgcc cctgtcgtag aaggtcttca gggtcgcgac
     1381 gtcctcgccc atcgggtagc cgttctcgac gccgacgagg atcacgcgct tcccggtctt
     1441 ctcgatccgc tccacgtcgc cgggagtccg ggccagctcg cacaggccgg ggtgcatcgc
     1501 ggtcagccgg tcgatggcat cgaaacggtc gattgcctcc gcgtacaccg ccttgtagcc
     1561 agccggcgtg aagtcctgcc gctgccctgt gtagaccgcc aggaagaccc cgtccatgcc
     1621 gccggccttc atcttcggca agtcgcactt gagccgcgtc ggcttgccgg ggtcgagcgc
     1681 cggggtggca taggttgcgc cgaggatgtc gacgtgcgaa tcgacagtga acacgcgatc
     1741 gtggattgcg cgggccttgg ccagcagctc ggcctccgag gaagcctgct gggcggcagc
     1801 cccggcaaat gtcacgacca gcgtggcgat ggcgacggcg atgaggtgtc tggccatggc
     1861 agctcgtagg ttctcagttc tgagtcgtca gttgacgtac tgtcactaga cctggaggtc
     1921 acatcggaaa ccgctctctg tgatctctgt gcctctgtgg ttgatttccg tctttcggcc
     1981 gggtgtcaga gttgcgccgc cgcgacgcgc gcacagatct ccaccgcgcg ccggcaggcg
     2041 gcttcatcga tcccgtagtg tgtcaccacg cgcaaccggc cgccgccgaa gtcggaaacg
     2101 agcacgcctt cgcgctcgag cgccgcggcg tacgccgagg ccgcctcgac gcccggcagc
     2161 cggaagacga cgatgttcgt ctcgacctcg gcggggtcga gcgtgatgcc cgggatgcgc
     2221 gccaggccgt cggccaggat cttcgcgttc gcgtggtcct cggcgaggcg gctcaccatc
     2281 ccggtgagcg cgacgattcc cggggcggcg atcacgccgg cctgcctcat gccgcccccg
     2341 agcatcttgc gcatccgccg cgcgcgatcg acgaaggccg acgagccgca gatcagcgac
     2401 ccgacgggcg cggccagccc cttcgacagg cacagctgca ccgagtcggc gtgccgcgtc
     2461 cagtcggtga ccggccgccc ggccgccagc gccgcgttga agaggcgcgc cccgtccacg
     2521 tgcaccgcca cgccgtggcg ccgcgcgatc gcggccaccc cggcgaagta ctcgggcgac
     2581 accacccggc cgccgcaccg gttgtgcgtg ttctcgaggc agatcacgcc ggtcggcgcg
     2641 tagtggtagt agtggtagtg gtgcgccggc agccggacgg ccgcctccac cgcctcgagc
     2701 ggcagcgtgc cgtccgggcg cgtcggaacc gtgtgcagca cgagcccgcc gagcgcggag
     2761 gccgacccgt tctcgtagtg gaagatgtgc gactcgtcgc cgaggatcac ctcgcggccg
     2821 cgctcgcagt gagccagcag cgaggcgagg ttccccatcg tgccgctggc gaccagcagc
     2881 gcggcgtcca tcccggtcat ctcagccgcc agccgctcga gccgcccgac agtcgggtcc
     2941 tctccgtaca catcgtcacc cacctcggcc tccgccatgg cctggcgcat ggcggcggac
     3001 gggagagtga ccgtatcgct tcgaaggtcg atggtcatta ggattcaggg tttcggaatt
     3061 cgggattcgg gaacccactt gttcatccac tcgatgaact cccggtacca caaggcggcg
     3121 ttggcgggtt ggttgatcca gtggccctcg tccgggaagt acaccagccg cgagggcacc
     3181 tcgaggcgct gcagcgtcgt gaacagctgc aagccctcgc caatcggcac ccggaaatcg
     3241 agctcgccgt gggtgaccag cgtcggtgtc ttgaagttct tcgcgtaggt gtgcggcgag
     3301 aacttcgcgt atccctcggg attcgtccag ggggttccct tgatgtccca ctcggggaac
     3361 cacagctcct cggtcacgcc ccacatgctg gtgaggttgt acacgctcgc gtgcgagacg
     3421 atggcgcgga agcgggtcgt gtggccgagg aaccagttca tcatgtagcc gccgaacgag
     3481 gcgccggcgg cccccacgcg tccccgctcc acgtacggca gcgcctcggc gaagtcgagg
     3541 cccttcatca ggtcgtcgta gaccttgccg ccccagtcgc cgctgatctc gtccacgaac
     3601 tgctggccga agccggtcga gccgcgcggg ttcggcatca ccacgacgta tcccgcggag
     3661 gcgaagacct gggcgttcca gcggaaggtc cagccgtcgt gccaggcgct ctgcggcccg
     3721 ccgtgcacga ggtacagcag cgggtacttg cggccgtcgg caaagtcggg cggcttgacg
     3781 atccaggcct gcacccgggc gccgccggcg ccctcgaacg tcgcgctctc gccctcgcgc
     3841 agcttgaagg ccggcaggcc ggggttcgcg ttggtcagcg gcttcgccgg cacctggccg
     3901 ttggcaggcg cggcggcaag gtcggccacg taaaggtcgg ccggcgaggt caacgtgttg
     3961 cggctgaaga cgagggtcct gccgtcgcgc gacagctgca ggtcgccgtt ggtgcccgac
     4021 gcgatgagcg ggaccgggtc gcccccgtcg agccgcagcc ggaacacgtc gctccggccc
     4081 tcgttctccg ccgtcaagta gatggaccgt gaatcgggcg cccacacgta gctgtcgggg
     4141 gaccggtccc agccgacagt ggtggatcgg tgctcgcccg ccttcctgtc gtacagcatc
     4201 aactgccagc ggtcggcctc gaaccctgcg cggtgctgcg cccggtaggc gatgaagcgg
     4261 ccgtccggcg agtagagcgg gccgccgtcg gccgcgggat tggtcgtgat ccgcctggcc
     4321 tcggccttcg ggtcggcaag atcgagcagg aacaggtcgc tgttggtgct gatcgcctcg
     4381 accttgtcgg tcttccgcac gaaggccagt tcccgagagt cgggcgagaa cgcgtagtcg
     4441 gtggggccgc ccagcgagaa gggcggcacg tcggcgggtc ccggcgtcag gtcgcgcggc
     4501 gcggccgatc cgtccgcggg gacgaggaac aggtggctga accgtccgtc cttccaggag
     4561 gtccagtgac ggaacatcag gccgtcgatc acgcgcgcct tgctcgcccg cccctccgct
     4621 tccttcagct tctgctcgtt gcaggcgagc gacccgcact cggggtacac atccgatacg
     4681 aagaggatcc acttgccgtc cggcgaccac gtgacgccgc tggcctcggt ggcgaggctg
     4741 gtgagcttgc gcggctcgcc aaccggcccc gcctcgccga caccgaccag ccacacctgc
     4801 gagctgccgt cgcgcgtcga gacgaacgcc agcgtccggc cgtcgggcga ccagcgcggc
     4861 gtgtcgtccg atcgcggcga ccgggcaatc gcaaccggct tcccccccgg caccggcagc
     4921 atccagatgt cgctgttccg gctgttccgc tcgaggctga cctcggtcac cacgtaggcc
     4981 actcggcggc cgtcgggcga gagctgcggg tcggagaccc gatcgagctt cagcatctcc
     5041 tccacggtga accggggaag gctctggggc gcggccggcg acgacccgac ggcaagcagg
     5101 accgcggcga cggcgagacg gcaggtcgaa cgcatcgatc cctccaagac cgcgcaggat
     5161 accacgcagg aatcaggatt caggaatcag gatccaggaa tcgggaatcg ggaatcagga
     5221 ttcggattca gggttcgggg ttcgggctcg ccccttgcac cccaacaggt cggccgccgc
     5281 aacgcgcgcg gccgcccccc gaagtacgcc cgtttccgcg ccgccggcgg cagcaccgcc
     5341 gggcgcgttg gcaggctctg cccaccctgg ccctggggat gttgcagcaa tcgcgatccg
     5401 cgatcgggca gttcgtgtcg gtccggcagg accattggcg cgtgctcgac gtcgtccgcc
     5461 acgacggctg cgagtcgtgg cggctcgagg gggtccgccc atccaaccgc ggagagcgcc
     5521 cgacgctgct cgtgccgttc gaccgcccgc ggccgctccc ggcgcgcgac cttcgcggac
     5581 gggcagtggg cgccaggcgc ggcctgctcg cgctgcgcgc gctggctgcc gtctcgacct
     5641 cggtggggca gttgagagcc gccgcggcgg ccacgatcga gatcctcgac taccagctcg
     5701 agccggtcct cgcatgcctg cagggcgcct cgcggctgct cctggccgac gaggtcgggt
     5761 tgggaaagac ggtacaggcg gcgctggtac tggccgagct gtcgtcccgg ggcgaggccg
     5821 ggcgcgtgct ggtgctgacg ccagccgccc tcgccgcgca gtgggccgag gagctgtccc
     5881 agcgcttcca gctcgagccc accatcgtcg aacgctcgac gctggcgcgc ctggcgtcgt
     5941 ggtcgtcgga cgaggagggc ccgtgggcgc ggctcccgct ggcgatcgcc tccatcgatt
     6001 tcgtcaagca gcccgaggtc ttgcggggga tgggcggcgt ccgctgggac ctgctggtcg
     6061 tggacgaggc gcacgcctgc gtccaggcgc cctatcgtgc ggcggccctc gggtggctgg
     6121 ccagccgggc gcggcgcctc gtgctcctca cggcaacgcc ccatcccggc gatgaggcgg
     6181 cgttccaggc gctttgccac gtgggccggc ttgcgggcga cccgccgatt gcgatgttcc
     6241 gccgcacccg ggccggcctc gggggcccgc ccacccgcag ggtgcgggtc gtgcgcgttc
     6301 gcattggcga ggccgaggcg cggatgcacc gcgcgctgct ctcctacgcc cggcgggtct
     6361 ggcaccgtgg cggagcctcg tctccggagc ctgcccccgc ccgcctcgcg atgaccgtcc
     6421 tctgcaagcg ggcagcctcg ggcccaggcg cgctgctcgt gtcgctgagg cggcgtctcg
     6481 aactgctcga ggcgccgtcg ccaaccgcgg tccagctgtc gctgcccctg gccgaggaag
     6541 ccggccacga ggacgaggcg cccggcgacg tgctgggggc cccgggactg gaggaccagg
     6601 aagaggagca gcgtgagctg cgacacctga tcgagatggc gcgcgaggcg tcggctcacg
     6661 atgccaagtc acgggccctt ctgcgccttc tgattcgtgc gcggcagcct gccgtcgtct
     6721 tcaccgagta tcgcgacacg ctcgaggggc ttgcggcaag ccttgcgccg ctgggcgcgg
     6781 ccgccacgct gcacggcggg ctcgacgggg cggcccgccg cgccgccctt gcacggttcg
     6841 cgtccggcgg cgcgcgcctc cttctggcca ccgacgcggc ctcgctcggc ctcaacctcc
     6901 aggcaacctg ccgcctcgtg gtcaaccacg atcttccctg gagcccgatg cgcctcgagc
     6961 agcggatcgg ccggctcgac cggatcggcc agcgccgcat ggtccacgcg atccacctgg
     7021 ctgcccgggg cacgttcgaa gaggaggtgc tcgcccgcct cgccctccgc gtggagaagg
     7081 tgcggtcctc catcggcttg ggagccagcc ccctggggac tgcagacgag ggacgcgtgg
     7141 ccggaacgat catcggtggt gacgccgagc ccgtggcaca ggccgcaagc cgctggcacg
     7201 aggccgggag ccgcgccgcg ttcgtcaagc cagacctcgg cgccgaggca cgggcggagg
     7261 caggccgcct gcggtttgtg agggcgctca cggcgccggc gccggggcgc cccgcacccg
     7321 acatcacctc gatcctcgat tcgtgcgagc ggggcgcgcc ctggcggttc gtgctgcgcg
     7381 ccgatcggtc tgcgcttgcc cccggcatgt acgcgctgta ccgggtgcga gtcatcgacg
     7441 gccgcggcgc cctgcttcac gacgaggtcg tgccgctgtt tcttccgctg cccctcatcg
     7501 ggcgtgggcc cgcgtctcgc gacgtttcgt ggcggtcgat ggctcctgcg ttcggaacca
     7561 tcgccggcac gctcggcgcc acggtgctgc agaaggtggc agcgcagctg cagcccgcga
     7621 tccgggcgca gcggactcgc gagtttcaaa tggcggcgct cgaagggcgc gcgcccgtac
     7681 ccgtgcaagc cggcctgttc gatcgccggg cggaaaaggc ggccgccgat gcggatgccc
     7741 gccgccgcgc ggctcgcttc gagatgtcgg cgcggctgcg ctcgctcgat gcggcgtgcc
     7801 ggccggtcct cgcctcgccg ccgcaaccgc tcctgctctt tctcgtcgcc cgcggccggc
     7861 ggccgcggtg gtcctgaacg gcgatgccag cgggcgtcag cggctcgctc gtctccgact
     7921 actacctgga cgaggtgctg cggcgcgagt tcgcaggaag actgggcgag gctggaagcg
     7981 acgccgccca tgccgccctg cgccgctggt ggcggtggac gggcaggcag ctggggccgg
     8041 tcactccgct gcggggcgtc ttcgacggtg ccgccgtccc gctcgcgcgc accctcgggt
     8101 ttcgcctgtc gtcgccacgg ccgtgcccgg gcgccggcgc gctcgccgcc gagatgttcg
     8161 cgcgcgacgg ccgttccgcg cacgcctggc cgctgatgct ggtggcgggc tggggcgaga
     8221 gcctctcgtc cgcctggcga tacaccgttc gagaaacgct cgcggccgga acgtcctggt
     8281 gcctctcgac caacggccgg cagctgcgcc tcaccgacac gctccggtcg tgctcgcgtc
     8341 gcttcctcga gttcgacctc gaaacggtgg cggaggatcg cgcggcgttc gtcccgttct
     8401 gggcgttgct gcgcgccgaa gctttcaaac cgcccgcgcc agccggcggg ccgaaggact
     8461 gcggcgaagc cggatccctg atcgaccgaa tcgtccgggc gtcggcaggc cagacgatcg
     8521 gcgtgtgccg gagcctcagg gccggcgtgc tcgaggccgt cggcgggatg atgtcgggtc
     8581 tcctgccgag gcgcgcactg agtggcgctg tggcctccag cgatctcgcg cagctctacg
     8641 agcaggcgct gacgatcgtc taccgcgtgc tcttcctcct gttcgccgaa tcgcggggcc
     8701 tcgtcccgtc gtggcatccc acctacaggc gcagctactc gatggaggcc gcgcgcgccg
     8761 tcgccgagtc gcacggcccg gcgcgcgggc tctgggaaac gctgcaggcg atgtcgcggc
     8821 ttgcgcacgc cgggtgccgc gccggcacgc tgcgcgtcac cccgttcaac gggcgcctgt
     8881 tcgcgccatc cctcacgccg gctggcgaat cggcccggcc cggcgacgag tgcgccaggc
     8941 ggatgctgct ctcgctgacg acggtcccgg gcgcccgcgg cgcgccccgt gcgcgcatcg
     9001 tgtaccgcga cctgggcgtg gaacaactcg gcagcgtgta cgaggccgtc ctcgactacc
     9061 ggcccgtgct gcggccagcg tcgtcagtag cacaggcgtc cccgcctgtg gctccagtgg
     9121 cacaggcgtc tccgcctgtg gtcccagtgg cacaggcgtc cccgcgtgtg gtcccagtgg
     9181 cacaggcgtc cccgcctgtg gtcccagtgg cacaggcgtc cccgcctgtg gtccacctcg
     9241 tgcccggctc cggtctccgc aaggccaccg gcaccttcta cacgccgtcg ccgatcacgg
     9301 agttcatcgt ccggcgcgcg ttgtggcccc tggcgcgcga cgccgctccg gagcagattc
     9361 tcggcctgcg cgtgctcgac ccctcgatgg gcagcggcgc gttcctcgtt gcggcttgcc
     9421 ggtacctcgc cgctgcttac gaagaggcgc tggtcaggac gggccgctac caccccagcg
     9481 acatcggcga tgccgagcgc aggacgtttc gccgcctcgt cgcccaacac tgcttgtacg
     9541 gcgttgacgt caaccccacc gcggtgcagc tggcgcggct ctcgctgtgg ctggccacgc
     9601 tcgcgcccga tcgccccctc tcgttcctcg accaccgcct gctcgtgggc gacagcctcg
     9661 tcggcgcgtc gctcgacgac gtggcgcggc ggcccttccc gggcggccgg ctgcgcggcc
     9721 cggccgccgg gactggcact ctgcccctct tcggcgatgg ggatgcggag gcagcggtgc
     9781 ggaccgtgct tccgtcccgc ctgaagctgg cgtccgagcc ggacgactcg gtgcaggccg
     9841 tgcgcgagaa ggaacaggtg ctcgcctcga tggaggggcc ggcgtcaccg ctggcccggt
     9901 ggaagctggt cgccgacgcg tggtgcgcgc tcgccttcgg cctcgactcg ggtgagcggc
     9961 cgcccccgtc gctgtttacg gcgcttgccg accggctggc cggccgggct tccccgctgc
    10021 cggtgctcga agccgaccgg tggatggccg aggtgtcgcg cacggcgagg gcgcatcgct
    10081 tcttccacta cacgctcgag ttccccgagg tgttcttcga cgagtcggga caaccgcgcg
    10141 cggacgccgg cttcgacgcc gtgctcggca acccgccgtg ggacatgatc cgcgacgact
    10201 cgggcgcaac cggtgccgcg ccgcttcatg ccggcgacgc ggccggcgat gccggcggcc
    10261 aacgggcggg cagaaactct ccggcggcca ggctggcccg ttttgcacgc gcctcgggcg
    10321 tgtaccacct gctctccgac ggccacagga accgcttcca gctgttcgtc gagcgcgcgg
    10381 tctgcctggc acggcgcggc ggccgggttg gcctcgtggt gccttcgggg ttgctgaccg
    10441 actgcggctg cgcacccctc aggcggctgc tcgtcgatcg ctgcgaggtg gacgggatgg
    10501 tctgcttcga caacgccggc cggatcttcc cgatccatcg cagtgtccgg ttcgtggcct
    10561 tcacggccac ctcgggcgtg ccggccgggc gcctgccgtg ccgggtcggc gagcgcgaca
    10621 cctcagtgct cgaccggatt cccgaccacg ccggcgccga gcacttcccc atcgtcttca
    10681 cgcgcgggtt tttggagcgt gtgtcggggg ccgacctggc ggtgcccgac gtgcgcagtg
    10741 ccgccgacct cgccctgctc gatcggctcg ttgccgccgc tcctgccctc ggctcgcccg
    10801 aggggtggaa cgcgcggttc ggccgcgagc tgaacgcgac cgacgacaac agcctgttct
    10861 ggaagggagc ctcgggcatg ccgatcgtcg agggaaagca cgtgcacccg ttccggctcg
    10921 agctgccggc cagcctcacg cggctccgcc cggaggccgc cgcggcactc ggcgctcgcg
    10981 gcgcctggtt cgcgcgcccg cggctcgcct accgcgacgt cgcctgcgcg acgaaccggc
    11041 tgacgtcgat cgccgcaatc gtcccggccg ggtatgcgac cgtccacacg gtcttctgcc
    11101 tgaagacgcg gcttgccgag gcagaccagt ggttcctctg cgcaatgctg aacagcctgg
    11161 tggtgaactt cctcgtcagg ctgcgcgtgg cgacccacgt gaccgccggc atcgtcgccc
    11221 gcctcccggt gccccggccg ccgagccact cgccggggtt caggcggatt gcgtggatgg
    11281 cccgtcgcct gggcggatcc acccatcccg agcgggatcg gcgctacgcg ctgctccagg
    11341 ccgaggttgc gcggctgtac ggactgacgg aggagcagct ggagcacgtg ctcgcagggt
    11401 ttccactgat cgaggacgag acgaagcggc aggtgcgagc cgcgtggcgc ccgccttcgc
    11461 accaggcgct gcggcgcggc aagccttgag ccctgagcct cgagccctta gtatggcggc
    11521 atgccggcgc cgtccatggc cctcacctcg atattgttcc tgggggggat cgcggcagga
    11581 gggcttggat cgctgctggg cctgggcggc ggcgtgttcc tcgtgcccct gctcgtcctc
    11641 gtgatggacg tgccgatgcg cgttgcggtc ggcatcagcc tcaccaccat catcgccacc
    11701 tccagcgtca ccacgtcggg ctcggtcggc cgctcgctga tcaacctgcg cctcggcatg
    11761 ctgctcgagg tggccaccac ggtcggcggg ctgacgggcg ggctggtggc cgtgctcatc
    11821 tcgcagaggc gcctgcagca gctgttcggc gtggtggcag cggcgggcgc ggcgatcatg
    11881 ctgagcagac tcggcaagcg gaacgtcctg ctcgacccgg cggccgagcc tggccgtctc
    11941 ggcgggcggt actacgagga ggagagccgg ggcgtcgtca cctaccaagt gaagcgcctg
    12001 ccgctggcgc tgttcgtgtc gctcgcggcc gggagcgttt cgtcgctcct cggaatcggg
    12061 ggcggcatgc tgaaggtgcc ggccctcaac gcctggtgcg gcgtgccgct gcgtgcggcg
    12121 gcggcgacga gcgccttcat gatcggcgtc accgcgacgg ttggcgccgt catctactac
    12181 gggcgcggcg agatcgtgcc gtggatggcc gcggctgcga tcctcggcgt gatggtgggg
    12241 tcgcgggcgg gcttccggat cggcgcggcc gccagcgcgc ggtggctcaa gctgctgctg
    12301 gcgatcgtgc tcgtgatcgt ttcgatcctg atgttcttga gggctcgatg agcgccgggg
    12361 aaggcaggga gctgcgggcg aacgccgacg accggctcga gcggtggctg ggcaagaccc
    12421 tgggcgcggg cacgatgctc gcgacggcgc tgctcgcact ggggttgctg ctgcagttcg
    12481 ccggcgtcgg cgccgaggtc tcggaccgct tgtcgcgggc aggactgatc gtcctgatgg
    12541 cgacaccggt cgccagggtg ctgatctcgg tggcggagta cgcgggacag cgcgactggc
    12601 ccttcctggc gctgacgctc accgtgctgg ggatgctgat ggtcagcttc ctccttggcg
    12661 tggggtgagt tcggggtaga gcagggcttc agccctgctg acacaccggc tggagcacaa
    12721 ctgtatgcga atcgcggtac tgggagctgg cgcggtcggc ggattctacg gggcgcgcct
    12781 cgcgcgggcc gggcaccagg tgtcgtttgt ggcccgcggc cgcagcctcg aggccctccg
    12841 atccagcgga atgagcgtgc gcagcgacct gggtgacttc gtggtctacc cggccgccca
    12901 gtccgatccc gtccggattg gcaccgtgga gctggctatc gtcgccgtga agacctactc
    12961 caacggcgag gcgctgccgc tgttgaagcc gctcgtcggc cccggaacca tggtgctgac
    13021 cctgcagaac ggcctggaca gcgcggccga ggttgcagcc gtcgtcggcg agggacacgt
    13081 gctggcgggc accacctaca tcggtgtgtc gctggaggcg ccgggagagg tcctgcacgt
    13141 cggaaccgcc cgtcggatcg ttttcggcga ggcgttcggt ccggcgcggg tcaccgagcg
    13201 ggtcgcacgg ctcgaggagg tgctgcgggg cgccgacatc cacgcggagg cggtcgccga
    13261 cagccggacg gcgctctggg agaagctcgt cttcctggcg ccgattgcgg ccctgaccgc
    13321 ggcagcgcgc ctccccatcg gaccgctcag gtcgagcccc gagttccgcg aagccgcgtt
    13381 gcgcgcgatg cgcgaggtcg aatcggtggc gcgagccgaa ggggtcgggc tgcccgccga
    13441 cgtctgcgag cagaagatgg cctacaccga cgcaacctcg ccggcgatgc gctcgtcgct
    13501 gatgatggac gtggtcgccg gcaggcagac ggaggtggag gcgctgctcg gcggcgtcgt
    13561 tcgccgcgcc cgccagcgcg gggttcccgt ccccgccatg cagacgctct acgccgttct
    13621 cgctcattcc gccaacgctg tcgcgctgcc cggtggagca agctgagcca gaggcgagct
    13681 cgcgagagca gggttgaagc cctgctccac cggtaaaatg gaggttcccg gaggagccat
    13741 gcgctcacgt ctggccacgc tcatcgtcgt ggcgatcgtc gccggaacct tcgtcatcgg
    13801 cttgatcgtc ggcgcgcagc gcgacgacga gaccgggccg gtggacctga tcgtcctgaa
    13861 cggccgcatc tacaccgccg acggctcggg acgcttcgcc gaagcgctcg ccgttcgcgg
    13921 caacaccatt ctccgggtcg gatccaaccg cgaggtgaag cggcttgcgg gccgctcgac
    13981 cgagaccatc gacgcgcacg gcgcctcggt gctgccgggc ctgaacgacg cacacctgca
    14041 cctggtgagc ggaggcctcg gcctgcagca ggccaacctg ctcgacgcct ccaccctgga
    14101 ggagatcggc gccgccatcg aggcctttgc cgccgcccat cccgatcgcc cctgggtgct
    14161 cggccgaggc tggtactaca cacccttccc cggaggcctc cccacgcgcc agcagctcga
    14221 tgccctggtc cccgaccgcc ccgcctacat gcgctgctac gacggccaca cggcgtgggt
    14281 caacacgctc gcgctgaagg cggcggggat cacccgcgag acgcccgacc cgcccggcgg
    14341 agtggttgtg cgcgacccgg ccaccggcga cccgaccggc gttctcaagg aggccgcgca
    14401 ggaactggtg gcccgccacc tccccgagtg gacgcgcgac gatcgcctcc gcgccatcgg
    14461 ggacgccatc gcgaaggccc acgagcttgg cgtaacgagc gcacagaacg ccggggtaga
    14521 cgaagccgag gtcgagctgt acgacgagct gctcaagaag ggtgacctcc agctccgtct
    14581 ctacaacgcg atctcgatcg agccgccgtt caccgcggcc gacgccgatc gcctggacgg
    14641 cctgtggaaa aggcgccccg acgacgcgct ggtcaagatg ggcgccgtga agctgatgct
    14701 ggacggggtc atcgagtcgc acaccgctgc catgctcgag ccctacgcca atcgcccggc
    14761 cacgggcttg ccgaacatgg acgcggccac gctgaacggc atcgtcgcga tgatggaccg
    14821 ccgcggctgg caggtgctgg ttcacgccat cggagacgcc gccgtccgcc agtcgctcga
    14881 cgccttcgag cacgccgccg cggtgaaccc ggtgccggcg cgcggccggc gccatcggat
    14941 cgaacacgtc gagacgctgc agccttccga cgtcccgcgc ttcggccggc tgggcgtggt
    15001 ggcggttcaa cagccgttcc acgggaaccc caacccgacg cagctcgcgg tctggagcgc
    15061 caatctcggt cccgagcggg cgtcgagagg ctgggcgcag cgcagcatcc tcgacgcggg
    15121 cgggcggctc gcgatgggca ccgactggcc cgtcgtcacg ctcgacccgc gctaccagat
    15181 caacatggcg atcaaccgca cgacgcccga ggggacgcca cccggcggat ggctgccgga
    15241 gcagcggttg acgctcgcct ctgccgtcga cgcgtacacc accggcgccg cctacgcgtc
    15301 gttcgacgag cggcgcaagg gtcagctggt ccccggcatg ctcgccgaca tcgtcgtcct
    15361 ctcgaccgac gtcttcgcga atgccccgga gaagttcctc gacgcggagg tcgcggtgac
    15421 gatcttcgac ggcaaggtgg tctaccggaa gacgcagcca tgaacgccaa gcccccgaaa
    15481 cgacccgtcc tgctggccgg ctcctgggag gagtcggcta ccccgctcga cgtcgtcaac
    15541 ccttacgacg ggacggtggt ggcctccacc ttcgccgccg gcgaagcgga gctggagcgt
    15601 gccacgcagg cggccgtggc cgcggcgccc ggcatgcgcc gcctcgccgc ttacgagcgg
    15661 gcggagatcc tcgcgcgggc gggtgacgag ttgcggcgcc gccgcgagga gatcggccgg
    15721 acgatcgccg ccgaggtggg caaggcaatc cgcgacgcgc tggccgaagc cgaccgggga
    15781 gtgcaaacct ttcaagcggc ggccgaagag gcccggcgca tcgccggcga ggagatcccg
    15841 atcgatctcg cgccgcatgg aaaggggcgc ctggccttca cgcggcgctt tccgatcggc
    15901 ccggtggcgg ccatctcccc gttcaacttc cccttcaacc tcgccgcgca caagctggcg
    15961 ccctccattg cggccggcaa cccggtcgtg ctgaagccgg cgtcgaagac gccgctgtcg
    16021 gcgctgatcc tggccgaggc gctcgaccgg gccggcgtgc caaagggcgc gctcagcgtg
    16081 ctgccgatgt cccgcgcgac cggcgagcgg atggtgaccg acgaccgctt caagctcctc
    16141 accttcaccg ggtcgtcgtc ggtcgggtgg ggaatgaagg cgcgcgcggg ccggaagcgc
    16201 gtcctcctcg agctcggcgg caatgccggg gtcgtcgtgg acgaaacggc cgacgtggcg
    16261 cacgcggtgg cgaggatcgc cgccggcggc ttcgcgctgg ccggacagag ctgcatctcg
    16321 gtgcagcggg tgtacgtgca ccggcggctg tacgagcggt tcgccgcgat gctggtcgag
    16381 aaggtcgagg cgctgaaggt cggcgacccg ctggatccgg cgaccgatct cggcccgatg
    16441 atcgacgagg acgaggccgg gcgggtggac gcctggatac gggaagcggt ccgcgaagga
    16501 gcccgcgtcc tcaccggcgg gaggcggctg gggaaggcgc tctacgcgcc gaccgtcctg
    16561 gtggacgtgc ccccggacgc gaaggtgtgc gccgaggagg tcttcgcgcc gctggtgggc
    16621 ctcttcccgt tcgacgattt cgaccgcgcg gtcgaggagg tcaaccgctc gccctacggc
    16681 ctgcaggcgg gcgtcttcac caacgacctc cgcaacgcgc tcgccgcctt cgatcgcctc
    16741 gaggtcggcg gcgtgatggt gaacgacgtg tcgtcgtggc ggatggacgc catgccctac
    16801 ggcggcgtga agaactcggg cctcgggcgc gaggggccgc gctacgccat cgaggagatg
    16861 accgagacga ggatcgtcgt cttcgccccg tgacacccgc tcacggcttg ctc
//
LOCUS       DAOPQX010000038        16903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4055_length_16903_cov_5.403312, whole genome shotgun sequence.
ACCESSION   DAOPQX010000038 DAOPQX010000000
VERSION     DAOPQX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_16903_cov_5.403312"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PKK95_03595"
     CDS             <1..277
                     /locus_tag="PKK95_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17324.1"
                     /translation="PVLAMGAWLTSLGLSPVVPTTLAIAADRHHRFGGTIFSLLFTIG
                     TFGSMSFPLALGELSAAAGLRLGMMVPLGGAVGVAACAAAVLKGRNR"
     gene            complement(305..1717)
                     /locus_tag="PKK95_03600"
     CDS             complement(305..1717)
                     /locus_tag="PKK95_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17325.1"
                     /translation="MTEALSTRVAETGAPVHPLPACRLSRLIGREPDEWTVDDLVGVV
                     GALGIRLISLMHVGGDGWLKTLDFVPRDVPHLREVLSFGERADGSSLFGGLGVPVTAS
                     DLVLRPRLSSAFVDPFATYPTLVVFCGHFGRDGQPLPESPDTIVRKAHDRLQAEAGVE
                     LHALGEVEFFLGKRPEENDIYGAAERGYHASSPFVFGEALRRRAMVLLSEAGIPIKYG
                     HSEVGYIQADDVDPRIWEQHEIELALQPLPQAADSVLIAQWVLRNLARQAGWRCAFDP
                     ILRKGHAGSGLHFHLSPVVGGVHQKHSEPDGRLVGPSKWLIAGLVRYADALMAFGNRT
                     STSFVRLTQAKEAPSSVTWGRYNRKALVRLPIVAADAEGRSVSPETVEFRLPDGSAHP
                     HLLLAGIAQAMLAARDFADIDALLEKTSVGTTATQAIAVPRMFLEVGQGLRANRAPLE
                     AGDVFPPHVIERTIEALMEA"
     gene            complement(1785..3017)
                     /locus_tag="PKK95_03605"
     CDS             complement(1785..3017)
                     /locus_tag="PKK95_03605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOC17326.1"
                     /translation="MSVFDAIVVGAGPAGAAAARGLAAGGARVCLLERARFPRNKPCG
                     GGITTRALSRFPWLEAALPRIPTHWVSRLYLEAGDGHHIVLTSERPAVLLVRRLEFDE
                     LLARLAVEAGAELCEEAWVSQVGADDDRVVVQTRDGREFVGRYVVAADGVNGIVTRRL
                     GLHQGWDGEAVALDMMEEAPDARLRATDPATLWVAYGHGGADGYGYVFPKQGHVNVGI
                     GCLLSCFKEQVRLPPYEFQRGFVSELVARGVLEGASTREHFTPCHIPVGGPIGRTAAG
                     RVLVAGDAGGFVNAYTAEGIYYAMVTGELAAGAILAALGTGGPERRGQAARHYVRAWK
                     REIGAELRDARLIQKYLFRDSQRIASVVRGAGNHGDLPQAIIGYAMGAIPYAAARRRV
                     LSRFPRTALRLARIALFG"
     gene            complement(3102..5756)
                     /locus_tag="PKK95_03610"
     CDS             complement(3102..5756)
                     /locus_tag="PKK95_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC17327.1"
                     /translation="MANRRVFAFLTAAAAAAVVTPLATQPRGPAPEGGRAALAIALRQ
                     LGNVGTVLQTVAHPDDENSALLAMLDRGQGYRTTLLTATRGTGGQNEIGPELFEALSV
                     LRTSELEQVHRFDGTEQYFGRQIDFGYSFSVDETFEKWGRDEVLSDYVRMIRTLRPDV
                     VLTMRPDGQGGGEHHQAQARITGEAFRAAADPARFPEQIREGLRPWQARKLYYTGSYG
                     FRGEPPPPQGVTLLPVVTDVYDPELGATYAEVGGEARSYHKCQGMGQLLPLPGGQTSR
                     YRLGDTSLPGGVDRKDASLFDGIDTTLPGLAQYVAGTPPAALTSALAAIAGRAGEARR
                     AFESAGMDAAVAPLASGLSAIRELRGRLQALGLDPAAAFEIDFRLAREQDEFQEALLI
                     AQALRIDLLADDGLVVPGQPVNVQAIVANRGGSPVRVEAVKLFGFDGESSCPAEELKP
                     AAIYRCASALGIPASARLTTPYWRPIPGTARYEFDPGVPFGLPFAPTPFRARLDLSMG
                     GTTVAADLPVRFRYEGNIFSGEKRAEMLVVPRYSVVMTPDIAVVPAGSQQTAGEASPS
                     TRGLRVAVTNGTRGAAKASVSLEAPNGWIVEPAASPVDFAREDETETVRFLVTPPASV
                     APGSYTVRARVADDGGTYSAGYQVIEYPHVNRRHRVVEAETSIKAIDVKVKPGLRVGY
                     VMGVGDQVPPAIEQLGVELTLLGADDLAWGDLSRYDAIVTGVRAYERRADLRANNRRL
                     LQYAQAGGTLIVQYNKFEFNEAQYGPYPAKVSSNRVTGEEAPVQVLVPDHPVFNSPNR
                     IGEDAWAGWVQERGLYFLAPDKDPRYADLVSLEDPFPHNPGVKTGALVEARVGKGRWI
                     YLGLGLWRQLPAGTPGAYQLLANLLSLK"
     gene            complement(5767..6741)
                     /locus_tag="PKK95_03615"
     CDS             complement(5767..6741)
                     /locus_tag="PKK95_03615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOC17328.1"
                     /translation="MSPSEARQELMDRRRRLESVIEAGMAVPQIEHLLREVDSALERV
                     EVGSYGRCETCGDAIEPERLAADPLTRFCLDHLSRDEARALEQDLQLAGRVQRGLLPP
                     RLLVHQGWEIAYEYRPLGPVSGDYCDIVSPPAGDGALLVLFGDVSGKGVAASMLMAHL
                     HATARTLAGLDLPLPQVLDRANRVFCESTMESHYATLVCARLSPSGRVELSNAGHCPP
                     LLLRNGQATPLQAHGVPIGLFCIEEFRVEAIDLAPGDALVLYTDGITESVNQDGEPYG
                     EERLTQMLAGWLDQSSSELVAGCVAHARDFRGRSQRADDETVMVIRRI"
     gene            complement(6868..8340)
                     /locus_tag="PKK95_03620"
     CDS             complement(6868..8340)
                     /locus_tag="PKK95_03620"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOC17329.1"
                     /translation="MIFHSLTFVCFFAVTVALYWRLPHRWQNVLLLVASYTFYGWIHP
                     WFLALIFATTFVDYWSAVGIERWPAHRRRFLWFSIVSNVGLLGFFKYFGFFADNVVAS
                     AALFGWELSRPMVDIFLPVGISFYTFQSMSYTIDVYRGHTHARHGFLNMMVFVSFFPH
                     LVAGPIMRATNLLPQVERERRFSASSARDATLLIAWGFFKKLVIADNVGVIANKVFAL
                     AEPDFYMLWAGVFAFCIQIYADFSAYADIARGTAKWLGFDLVHNFDHPYLATGPTDFW
                     KRWNISLTTWFRDYVFFPLSFALSRRVDEGRRFGLRADLRIYAAATLLTFLLSGLWHG
                     ASWNYVIWGGYHGLLLVIARASASAWRPRRRVRKYLRPFQWAGMFALTNVGWLIFREP
                     ELSMIWRDLWLSPFQSTQLQHQAGVYLFLLAFLYSIPLWVQSLWVRFVPSDLAAAVDR
                     DEPDVRFGRVLGQGALAGVMFAAILVLRSYTSLDFIYFQF"
     gene            complement(8337..9434)
                     /locus_tag="PKK95_03625"
     CDS             complement(8337..9434)
                     /locus_tag="PKK95_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17330.1"
                     /translation="MPVVAAGASTLDRQAAASRRRALVRPVASLLLLAALAAVLPLGL
                     RAVNAFTATGKQPESYLPSAFAPGPRRPFVGSAVEGLRNVNPAYLVIGDSMAGTRVDH
                     RYLAKLTGRLVVPIYEPASASAYWYLAYKNWVVASGIRPRAVIFYFRDWMLTDPMFRL
                     NPQHLDRVALQREPELDRIVAQHAQGTFFRVHAWLRDAYQYDRVRAWLLPLLERAPVA
                     LAASRPKARRELLDRMNNEIFNLEGLREMAAADIGEADLERLDFDRNLPRSVLPELLK
                     LARENSTRLAFIRVQRRPEGNRPPRQSPELQEYTRKLKQYLEANGAYFHDEWGDPELP
                     LSIYRDGDHIRRDKEKHTTELLVRRNPGLFQ"
     gene            complement(9550..10701)
                     /locus_tag="PKK95_03630"
     CDS             complement(9550..10701)
                     /locus_tag="PKK95_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOC17331.1"
                     /translation="MPSEKELLALVALSYAAATDPAQWSAFLDAVARAAQASRPILFL
                     HPVDPSDAGHRSIVCARGYDEGVLEDYRQYYAARNIWLAQGRRLLRPGVARTSEMMCR
                     REVLLASEFYNDFLRPNAVSHALAATLAVEEGVALNLSVFREPGDPQFSDEDLQFLQA
                     LLPHLEQAAAIRRRTEGLETRARWCEAALDRLSGAVVLVDRRGKVLFANESARRLLGS
                     RDGLAIEREQLLAATPSLTAELRRLIARATGAGPDGDPRSGGAMAVPRRGNPRPLDVV
                     VAPLASSPPSMEARAAAALFLGDPSESPPSAHLGPALRCLYGLTPAEARVAELLVRGM
                     SPHEIASACGTSLETVRTQVKQVLSKTDTRRQSQLVALLLRTTAASLTD"
     gene            10805..11551
                     /locus_tag="PKK95_03635"
     CDS             10805..11551
                     /locus_tag="PKK95_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC17332.1"
                     /translation="MSLGALLVIHGSLVADGCVPLLDQPALRDRVRPIVYHRRGYDGV
                     ACSDFGIEAQAEDAVLALRASGLPRAHVLGHSSGASIALQLAVAHPGAVRSLILVEPA
                     IVGAVAAAGEVAAALSGILPRYAEGRHAEAVEALLSYCNPGGYREALEGRLAPGWFER
                     AVASSAVYFEGELPALAAWSVTRDAAARITVPTLLVCGADSPPVFHQTQALLAAWLPC
                     ARTVTIPGAGHQVPTAAPEALAEAIVSFLT"
     gene            11670..11846
                     /locus_tag="PKK95_03640"
     CDS             11670..11846
                     /locus_tag="PKK95_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17333.1"
                     /translation="MLSVFRGHHEKPHDEAEVALLRALLPHVAQALAIQAKTADLESR
                     ARWGEESLEHLQSG"
     gene            12082..12414
                     /locus_tag="PKK95_03645"
     CDS             12082..12414
                     /locus_tag="PKK95_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOC17334.1"
                     /translation="MVCPFRSPALLGRGAAIVFVSDPDAQPRSLNGALRRLYGLTHAE
                     ARVAERLCCGESLEEISDDTATSLNTVRTHVKRALMKTGTTRQAQLVALALGLAASPD
                     PSQPQPDR"
     gene            12518..12952
                     /locus_tag="PKK95_03650"
     CDS             12518..12952
                     /locus_tag="PKK95_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17335.1"
                     /translation="MAPVQADKTAEAMAELSKEIRSAVGDRPLTAEEVSESRDRQIRS
                     LAGRWETAHDVFHALDEIVTLGLPDDYYAAYPKKLRAVTPETANSAAKKFIHPDQLVW
                     VVVGDRQKIEPVIRALNLGEVQLLEPEGAALTARAQEGAKVR"
     gene            complement(12986..13753)
                     /gene="cobA"
                     /locus_tag="PKK95_03655"
     CDS             complement(12986..13753)
                     /gene="cobA"
                     /locus_tag="PKK95_03655"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOC17336.1"
                     /translation="MSGGDGSNVGTVYLVGAGPGDPRLLTLRGLELLRGADTVVFDAL
                     VDRALLEHCRPGVRLFVAGQRDDPHVLAQDAINRLIVEEARAGRTVVRLKGGDPFVFG
                     RGGEEAEVLAAAGVRYEIVPGVSAGTAVPACAGIPATHRGYTSELVFLTGQQCESKPT
                     PVNWSLYGPSSATLVIFMGIASLADVAGSLLAHGRSPSCPAAVIENGSTTAQRTITAP
                     LSRIADEAATAGIQAPAIVVIGEVVGLRDRLKWFPEG"
     gene            complement(13771..15378)
                     /locus_tag="PKK95_03660"
     CDS             complement(13771..15378)
                     /locus_tag="PKK95_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOC17337.1"
                     /translation="MSGPTRLSDLARRDREEVTRVDRAPLAPLPPPLDDSGTRPPFAP
                     LSDQARERFDGALDGTLALNLPVPAGPDEERALVERFLAGLDKLFERENNWTFLEPLV
                     LTMEHCAKCQTCAEACHVFEASGGEEIYRPTFRAEILRRLYFKHVKNGGLLAAWLHGD
                     ITLNWPLVARLAELSYRCNLCRRCAQACPIGADNGLVAHELRKLFSQELGIAPKELHD
                     NGSMLQLSAGSSTGMTPVVVRDNVEFVDEDVSEKTGLEVKTPWDVEGADVLLVHNAGE
                     IMAWPENPGAFAVILNAAGISWTMSSELAGYDSINYGLWYDDAQFARVAIRHAEAARK
                     LKVRKIVLGECGHAHKALAVVADRVLSGDLDIPRESCLTLFRDIVASGRLKLDPSRND
                     FPVTLHDPCNVVRLMGVVEPQREILRAICPQFREMAPHGVDNYCCGGGSGFAIMSGHN
                     FRDWRLQVSGRKKVEQALEAFADCLDPSIPKYICAPCSNCKGQFRDAIEYYGLWKKHR
                     ILYGGLAELIVNAMTDVKPGFIEWEWH"
     gene            complement(15375..16292)
                     /locus_tag="PKK95_03665"
     CDS             complement(15375..16292)
                     /locus_tag="PKK95_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17338.1"
                     /translation="MTVLTFLLIWLGLGVFAAATAARAVRYARAPVHLRWELYPVPHE
                     APERAAHGGSRYEELEWWTRPQRRHLLGELAYMLPEMLLLAGLRQHNRRLWYRSYPFH
                     LGLYLVAAACALLAAGALLDVAGVPLGDGAANALTAACDLLGTAGVVLSAGGALALLH
                     ARLTDPKLAAYSAPADYFNLALFAGVLPLAGLGFLLEPAPSLLVAARVARFDTAAPLH
                     PALAAGLALASLTAAYIPLTHMSHFVGKWFTYHNVRWDDGPTSERVRAKMTEYLTYRP
                     TWSASHVQADGTKTWRDVVAGTPPRRGQR"
     gene            complement(16289..16606)
                     /locus_tag="PKK95_03670"
     CDS             complement(16289..16606)
                     /locus_tag="PKK95_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOC17339.1"
                     /translation="MATIVLNGRTYAVDDDGFLEDPGIWNREVAEDLATTEGISGLNE
                     EQWKVVNYLREYYLQAGMAPMIRKLCKETGFKLAQIYEMFPSGPAKGACKVAGLPKPT
                     GCV"
BASE COUNT         2583 a         5874 c         6035 g         2411 t
ORIGIN      
        1 tccggtgctc gcgatgggcg cctggctcac atcgctcggc ctctcgccgg tcgtgcccac
       61 cacgctggcg atcgccgccg accgccacca ccgattcggc ggcaccatct tctcgctgct
      121 cttcacgatc ggcaccttcg gctcgatgtc cttcccgctg gccctggggg agctgtccgc
      181 ggcggcaggc ctgcgcctcg gcatgatggt gccgctcggc ggagcggtgg gggtggcggc
      241 gtgcgcggcg gcggtactga agggcaggaa tcggtaggcc gcagggcgac cacaagggtc
      301 gcccctacgc ttccattagc gcctcgatcg tcctctcgat gacgtgcggg gggaagacgt
      361 cgcccgcctc gaggggcgcg cggttcgcgc gcagcccctg ccccacctcc aggaacatcc
      421 ggggcaccgc gatcgcctgc gtggccgtcg tgcccaccga cgtcttctcc aggagcgcgt
      481 cgatgtcggc gaagtcgcgc gcggcgagca tcgcctgcgc gatgccggcg agcagcaggt
      541 gcgggtgggc cgacccgtcg ggcaggcgga actccacggt ctcggggctg accgaccgcc
      601 cctcggcgtc ggcggcgacg atcggcagcc gcaccagcgc cttccggttg tagcgccccc
      661 acgtgacgct gctcggcgcc tccttggcct gcgtcagccg cacgaacgag gtggacgtcc
      721 ggttgccgaa ggccatcagc gcgtccgcgt agcgcaccag ccccgcgatc agccacttgc
      781 tcggtcccac caggcgcccg tccggttcgc tgtgcttctg gtgcacgccg ccgaccaccg
      841 gcgagaggtg gaagtgaagg ccgctgccgg cgtgcccctt gcgcaggatc gggtcgaacg
      901 cgcagcgcca gcccgcctgg cgggcgaggt tccggagcac ccactgcgcg atcagcaccg
      961 agtcggcggc ctgcggcagg ggctgcagcg ccagctcgat ctcgtgctgc tcccagatcc
     1021 tcgggtccac gtcgtcggcc tggatgtaac cgacttcgct gtggccgtac ttgatcggga
     1081 tgccggcctc cgagagcagg accatcgccc ggcgccgcag cgcctcgccg aagacgaacg
     1141 gcgagctggc gtggtagccg cgctcggccg cgccgtagat gtcgttctcc tcggggcgct
     1201 tgcccagaaa gaactccact tccccgagcg cgtgcagctc gacgccggcc tcggcctgca
     1261 accgatcgtg cgccttgcgg acgatggtgt cgggcgactc gggaaggggc tggccgtcgc
     1321 gtccgaagtg gccgcagaag accacgaggg tcgggtaggt ggcgaaggga tcgacgaagg
     1381 cgctggaaag gcgcggccga agcaccaggt cggacgccgt caccgggacg ccaagcccgc
     1441 cgaacaggct cgagccgtcc gcgcgctcgc cgaacgacag cacctcgcgc aggtgcggca
     1501 cgtcgcgcgg cacgaaatcg agcgtcttca gccagccgtc gccgcccacg tgcatcaggc
     1561 tgatcagccg gatgccgagg gccccgacga cgcccacgag gtcgtcgacc gtccactcgt
     1621 ctggttcgcg cccgatcagg cgggacagcc ggcaggcggg caggggatgg acaggggccc
     1681 cggtttcagc gacgcgggtc gacagcgctt cggtcatggc cggatcggct ccaggtgtcg
     1741 agggatgaga aacggcgcgg gggaagccat tataggccag ggcgtcagcc gaagagcgcg
     1801 atcctggcca ggcgcagggc ggtgcgcggg aaccgtgaga gaacgcggcg gcgtgcggcg
     1861 gcgtacggga tggcgcccat cgcatatccg atgatggcct gcgggaggtc gccgtgattg
     1921 ccggctccgc gcaccaccga ggcgatgcgc tgcgagtcgc ggaacaggta cttctgtatg
     1981 aggcgggcat cgcgcagctc ggcgccgatc tcccgcttcc aggcgcgaac gtagtggcgc
     2041 gccgcctggc cgcgccgttc cggcccgccc gttccaaggg ccgcgaggat ggcgccggcc
     2101 gcaagctccc cggtgaccat cgcgtagtag atcccttcgg ccgtgtaggc gttcacgaac
     2161 ccgccggcgt ctccggccac gagcacgcgc ccggcggcgg tccgcccgat cgggcccccg
     2221 accgggatgt ggcagggtgt gaagtgctcg cgcgtcgagg ctccctccag cacgccccgc
     2281 gcaaccagct cgctcacgaa gccgcgctgg aactcgtacg gcggcagccg cacctgctcc
     2341 ttgaagcacg acagcaggca cccgattcca acgttgacgt ggccctgctt cgggaacacg
     2401 tacccgtagc cgtcggcgcc gccgtggcca tacgccaccc acagcgtcgc cggatcggtg
     2461 gcgcgaaggc gggcgtcggg cgcctcctcc atcatgtcga gagcgacggc ctcgccgtcc
     2521 cagccctggt gcagccccag gcgccgcgtg acgatgccgt tcacgccgtc ggcagccacc
     2581 acgtaccgcc ccacgaactc gcggccgtcg cgcgtctgca cgaccacccg gtcatcgtca
     2641 gcccccacct gggagaccca cgcctcctcg cacagctcgg cgcctgcctc caccgcgagg
     2701 cgcgccagca gttcgtcgaa ctcgagccgc cggacgagga gcacggccgg ccgctcggat
     2761 gtcaggacga tgtggtgacc gtcgccggcc tcgaggtaga gacgggagac ccagtgggtg
     2821 gggatgcggg gcagcgcggc ctccagccac gggaagcgcg agagggcgcg cgtggtgatg
     2881 ccgccgccgc aaggcttgtt gcgcggaaag cgggcgcgct cgaggaggca gacacgcgct
     2941 ccgccggcgg cgagcccgcg agcggcagcc gccccggcag ggcccgcgcc gacaacgatc
     3001 gcgtcgaaga ccgacacgac ggcattgtag caaggcggct caaggctcac ggcttcagcc
     3061 tcaaggctca gggcttgagc cgtgagccat gagccgtgag cctatttcag gctcaagagg
     3121 ttcgccagca gctgatatgc ccccggcgtc ccggccggga gctggcgcca cagcccgagg
     3181 cccaggtaga tccaccgccc cttgccgacg cgcgcctcga ccagtgcgcc cgtcttcaca
     3241 ccggggttgt gcgggaacgg atcctccagg ctgaccaggt cggcatagcg cggatccttg
     3301 tctggtgcca ggaagtacag gccgcgctcc tgcacccatc ctgcccacgc gtcctcgccg
     3361 atgcggttgg ggctgttgaa caccgggtga tcgggcacga gcacctggac cggcgcctct
     3421 tccccggtca cccggttcga gctgaccttc gccgggtagg gcccgtactg cgcctcgttg
     3481 aactcgaact tgttgtactg aacgatcagc gttccgccag cctgcgcgta ctggagcagc
     3541 cggcggttgt tcgcgcgcag gtcggcgcgc cgctcgtagg cgcgaacgcc ggtgacgatg
     3601 gcgtcgtagc gcgagaggtc gccccacgcg agatcgtcgg caccgagcag ggtcagctcg
     3661 actcccagct gctcgatggc gggcggcacc tggtcgccca cgcccatcac gtaaccgacg
     3721 cgaagccccg gcttcacctt cacatcgatc gccttgatcg acgtctcggc ctcgacgacc
     3781 cggtgtcgcc ggttgacgtg cgggtactcg atcacctggt agccggccga atacgttccg
     3841 ccatcgtcgg cgacgcgcgc ccgcacggtg tagctgcccg gcgccacgct ggcaggcggc
     3901 gtcacgagga agcgaaccgt ctcggtttcg tcctcgcgcg cgaagtccac gggcgaggcc
     3961 gccggctcga cgatccaccc gttaggggcc tcgagcgaga cgcttgcctt cgcggccccg
     4021 cgggttccgt tggtcaccgc gacccgaagc ccgcgcgtcg aaggcgaggc ctcgcctgcc
     4081 gtctgctgcg atccggccgg gacaaccgcg atgtcgggcg tcatgacgac cgagtaacgg
     4141 gggacgacga gcatctccgc gcgcttctcg ccgctgaaga tgttcccctc gtagcggaac
     4201 ctcaccggga ggtcggccgc gaccgtcgtt ccgcccatcg acaggtcgag ccgcgcccgg
     4261 aacggcgtcg gcgcgaacgg caggccgaag gggacaccag gatcgaactc gtagcgggcg
     4321 gttccgggaa tgggccgcca gtagggcgtc gtgagccgcg cgcttgccgg gatgccgagg
     4381 gccgaggcgc agcggtagat ggcggccggc ttcagctcct ctgccgggca agacgactca
     4441 ccatcgaagc cgaaaagctt gaccgcctcg acgcggacgg gcgaaccgcc gcggttggcg
     4501 acgatggcct gcacgttcac cggctggccc ggcacgacca gcccatcgtc ggccagcagg
     4561 tcgatgcgca gcgcctgggc gatgagcagg gcttcctgga actcgtcctg ctcgcgcgcc
     4621 aggcggaagt cgatctcgaa ggccgcggcc ggatcgagcc caagcgcctg cagcctgccg
     4681 cgaagctcgc ggatggcaga aagcccagaa gccagcgggg cgacggcggc gtccatcccg
     4741 gcgctctcga atgcgcggcg cgcctcgccg gctcgccccg cgattgcggc cagcgcggag
     4801 gtcagagccg cgggcggcgt accggcgacg tactgcgcga ggccgggcag cgtggtgtcg
     4861 atgccgtcga agagcgaagc gtccttgcgg tcgacgccgc cgggaagcga ggtgtcgccg
     4921 aggcggtagc gcgaggtctg gcccccgggc agcggcagca gctgccccat gccctggcac
     4981 ttgtgatagc tgcgcgcctc gccgcccacc tccgcgtagg tggcgcccag ctccgggtcg
     5041 tagacgtcgg tgacgacagg cagcagcgtc acgccctggg gcggcggcgg ctcgcctcgg
     5101 aagccgtagc tgccggtgta gtacagcttg cgcgcctgcc acgggcgcaa cccctcgcgg
     5161 atctgttcgg ggaaacgggc cgggtccgcg gcggcccgga aggcctcgcc cgtgatgcgg
     5221 gcctgggcct ggtgatgctc acctccgccc tggccgtcgg gccgcatcgt gaggacgacg
     5281 tccgggcgca gcgtccggat catccggacg tagtcggaga ggacctcgtc gcgcccccac
     5341 ttctcaaacg tctcgtccac gctgaacgag tagccgaagt cgatctggcg gccgaagtac
     5401 tgctcggtgc cgtcgaagcg gtgcacctgc tccagctcgc tggtgcgcag aacgctcaaa
     5461 gcctcgaaca gctcggggcc gatctcgttc tggccgccgg tgccgcgcgt ggcggtcagc
     5521 agcgtggtcc gataaccctg gccccgatcg agcatcgcga gcagcgcgct gttctcgtcg
     5581 tccgggtggg cgaccgtctg caggacggtg ccgacgttgc caagctggcg cagcgcgatg
     5641 gcgagggccg cccggccgcc ctctggcgcc gggccgcggg gctgggtggc gaggggggtg
     5701 acgacggccg ccgcggcggc ggcggtcagg aacgcgaaga ctctgcgatt ggccacgagg
     5761 gaaattctat atcctcctga ttaccatcac cgtctcgtcg tcggcccgct gcgaccggcc
     5821 gcggaagtcg cgggcgtgag cgacgcagcc ggcgaccagt tcggacgagg attgatcgag
     5881 ccagccggcg agcatctgcg tgaggcgctc ctcgccgtac ggctcgccgt cctggttgac
     5941 ggactccgtg atcccgtcgg tgtagagcac cagcgcgtcg ccgggcgcga ggtcgatggc
     6001 ctccacgcga aactcctcga tgcagaagag gccgatcggg acgccgtgcg cctggagcgg
     6061 cgtggcctgg ccattccgca gcaggagcgg cgggcagtgg ccggcgttcg acagctcgac
     6121 gcgcccggag ggcgacagcc gcgcgcagac cagcgtggcg tagtggctct ccatggtgct
     6181 ctcgcagaac acgcggttgg cccggtcgag cacctgcggg agcgggaggt cgaggccggc
     6241 gagcgtcctc gcggtggcgt gaaggtgggc catcagcatg gaggcggcca cacccttccc
     6301 cgagacatct ccgaacagca cgagcagggc gccgtcgccg gcgggcgggc tgacgatgtc
     6361 gcagtagtcg ccgctgacgg ggccgagcgg gcggtactcg taggcgatct cccatccctg
     6421 gtgcacgagc agcctgggcg gcagcagccc ccgctgcacg cgcccggcca gctgaaggtc
     6481 ctgctcgagg gcgcgggcct cgtcgcggct caggtggtcg aggcagaacc gggtcaaggg
     6541 atcggcggcg aggcgctcgg gctcgatggc gtcgccacag gtttcgcacc ggccgtagct
     6601 tcccacctcg acccgctcga gcgcgctgtc cacctcgcgc agcaggtgct cgatttgcgg
     6661 aaccgccatc cccgcctcga tgacgctctc gaggcgccgg cggcggtcca tcagctcctg
     6721 gcgggcttcg cttggcgaca ttcgggtacc cccttgtcag tgtaccgcac gggagagctc
     6781 tggcagtcca ggaagtcccg aagtcacgag gtcacgaagt cggctggtca agtccacgaa
     6841 gtcctggagt ccggactgac ggctagacta gaactggaag tagatgaaat ccaacgacgt
     6901 gtagctgcgg agaacgagga tcgcggcgaa catcacgccg gccagcgcgc cctggcccag
     6961 cacgcggccg aaccggacgt ccggctcgtc gcgatcgacc gccgcggcca ggtcggatgg
     7021 cacgaaccgc acccacaggc tctggaccca cagcgggatc gagtacagga aggccaggag
     7081 gaagaggtag acgccggcct ggtgctgcag ctgcgtggac tggaacgggc tcagccagag
     7141 gtcgcgccag atcatcgaca gctcgggttc gcggaagatg agccagccga cgttggtgag
     7201 cgcgaacatg ccggcccact ggaaggggcg gaggtacttg cgcacgcgcc gccgggggcg
     7261 ccaagccgag gcggacgcgc gcgcgatcac gagcagcagc ccgtggtatc ccccccagat
     7321 cacgtagttc cagctggcgc cgtgccagag gcccgacagc aggaaggtga gcagggttgc
     7381 cgcggcgtag atccgcagat cggcacgcag gccgaaccgc cttccctcgt ccacgcgccg
     7441 ggagagcgcg aagctgagcg ggaagaacac gtagtcgcgg aaccaggtgg tgagcgagat
     7501 gttccagcgc ttccagaagt cggtgggccc cgtggccagg taggggtggt cgaagttgtg
     7561 gaccaggtcg aacccgagcc acttggcggt gccgcgcgcg atgtcggcgt aggccgagaa
     7621 gtcggcgtag atctggatgc agaaggcgaa gacacccgcc cagagcatgt agaagtcggg
     7681 ctcggcgagc gcgaacacct tgttcgcgat gacgccgacg ttgtcggcga tcaccagctt
     7741 cttgaagaac ccccaggcga tgagaagcgt cgcgtcgcga gcggaggacg ccgagaagcg
     7801 gcgctcgcgc tcgacctgcg gcaggaggtt ggtggcccgc atgatcgggc cggcgaccag
     7861 gtgcggaaag aacgagacga acaccatcat gttcaggaag ccgtgcctgg cgtgcgtgtg
     7921 gcccctgtac acgtcgatgg tgtagctcat cgactggaac gtgtagaacg agatcccgac
     7981 cggcaggaag atgtcgacca tcgggcgcga cagctcccag ccgaagaggg cggcggaggc
     8041 gaccacgttg tcggcgaaga agccgaagta cttgaagaag ccgagcaggc cgacgttgct
     8101 gacgatgctg aaccagagga agcgccgccg gtgcgccggc cagcgctcga tgccgacggc
     8161 cgaccagtag tccacgaagg tcgtggcgaa gatcagcgcc aggaaccagg ggtggatcca
     8221 gccgtaaaag gtgtagctgg ccaccagcag cagcacgttc tgccagcggt gcggcaggcg
     8281 ccagtacagc gccacggtga cggcgaagaa gcacacgaag gtcaggctgt ggaagatcac
     8341 tggaacagcc ccggattgcg ccgcacgagc agctcggtgg tgtgcttctc cttgtcgcgc
     8401 cggatgtgat cgccgtcccg gtagatcgac agcggcagct cggggtcgcc ccactcgtcg
     8461 tggaagtacg cgccgttcgc ctcgagatac tgcttcagct tgcgggtgta ctcctgcagc
     8521 tcgggcgact gccggggcgg ccggttccct tcggggcgcc gctggacgcg gatgaacgcg
     8581 aggcgcgtcg agttctccct cgccagcttc agcagctccg gcagcaccga gcgcgggagg
     8641 ttcctgtcga aatcgagccg ctcgaggtcg gcctcgccga tgtcggcggc ggccatctcg
     8701 cgcagccctt cgaggttgaa gatctcgttg ttcatccggt cgagcagctc gcggcgcgct
     8761 ttcgggcgcg aggcggcgag cgcgaccggc gcccgctcga ggagcggcag gagccacgcg
     8821 cgcacccggt cgtactggta ggcgtcgcgg agccaggcgt gcacccggaa gaaggttccc
     8881 tgcgcatgct gcgccacgat ccggtcgagc tcgggctcgc gctgcagcgc gacccggtcg
     8941 aggtgctgcg gattgagccg gaacatcggg tcggtcagca tccagtcgcg gaagtagaag
     9001 atcaccgccc gcggcctgat tcccgacgcc accacccagt tcttgtaggc gaggtaccag
     9061 taggccgagg cgctggccgg ctcgtagatc ggcaccacca ggcgcccggt gagcttcgcc
     9121 aggtaccggt ggtccacgcg cgtcccggcc atggagtcgc cgatcaccag gtaggcgggg
     9181 ttcacgttgc ggagcccctc gaccgcgctg ccgacgaagg gccggcgcgg cccgggcgcg
     9241 aaggccgagg gcaggtagct ctccggctgc ttcccggtgg cggtgaaggc gttgacggcc
     9301 cggaggccaa gaggcagcac ggccgcgagc gcggcgagca gcagcagcga ggccaccggg
     9361 cggacgagcg ccctcctccg tgaggccgcg gcctggcggt cgagagtgga cgcgcctgcg
     9421 gccactacgg gcacggtctg ctgaatggag cctggatcct gtgtcacgtg ctgtcgcggt
     9481 tgccggcggg tcccggcaag acgcgcattg tacaacagcg ggaatcggga ttcggtgcgt
     9541 gtgcggggat cagtcggtca ggctcgcagc ggtcgtacgg aggaggagcg cgacgagctg
     9601 cgactgacgg cgggtgtcgg tcttcgacag gacctgcttc acttgcgtgc ggacggtctc
     9661 gaggctcgta ccgcaggccg aggcgatctc gtgcggcgac atccctctga ccaggagctc
     9721 ggcgacgcgg gcctcggccg gagtcaggcc gtacaggcac ctgagggcgg ggccgaggtg
     9781 cgcggacggt ggcgattccg agggatcacc gaggaagagg gcggccgccg cgcgcgcctc
     9841 catcgagggc ggtgacgagg ccagcggagc caccaccacg tccagcgggc gagggttccc
     9901 cctgcgcgga acggccatcg cccccccgga ccgcgggtcg ccgtccgggc cggcgcccgt
     9961 ggcccgggcg atgagccggc gcagctccgc cgtgagcgaa ggggtggccg ccagcaactg
    10021 ctcgcgctcg atcgccaggc cgtcgcgcga gcccagcagc ctccgggccg actcgttcgc
    10081 gaagagcacc ttcccgcgcc gatcgacgag aaccaccgct cccgacagcc ggtccagcgc
    10141 cgcctcgcac cagcgggcgc gcgtttccag cccctcggtg cgccgccgga tggccgcggc
    10201 ctgctcgagg tgagggagaa gcgcctgcag gaactgcagg tcctcatcgg agaactgagg
    10261 gtcgcccggt tcgcggaaga cgctgaggtt gagagcgacg ccctcttcca cggcgagtgt
    10321 cgccgccagc gcgtgcgaca ccgcgttcgg ccgcaggaag tcgttgtaga actcgctggc
    10381 gagcagcact tcgcgccggc acatcatctc gctggtccga gcgacgccgg gacgcaggag
    10441 cctccgtcct tgggcgagcc agatgttcct ggccgcgtag tactgccggt aatcctccag
    10501 cacgccctcg tcgtagcccc gggcgcagac gatggaccgg tggccggcgt cggacgggtc
    10561 gacgggatgc aagaagagaa tggggcgcga agcctgggcc gcgcgcgcga ccgcatccag
    10621 gaatgcagac cactgcgccg ggtcggtcgc ggctgcgtac gacagcgcaa cgagggcgag
    10681 gagctctttc tcgctcggca tgcctccacc ggcttcggat gaagggggcc gcagttgtat
    10741 ggggtgaacc cactgggcag tatactgccc gcagggcaat ccgccagatg aaagcgatga
    10801 ttggatgagc ttgggagcct tgctcgtcat ccacggctcg ctggtcgccg acgggtgtgt
    10861 cccgctgctg gaccagcccg cgcttcgcga tcgcgtccgg ccgatcgtct atcaccggcg
    10921 cggctacgac ggggtggcct gcagtgactt tggcatcgag gcgcaggcag aagacgcagt
    10981 ccttgccctt agggcgtcag gcctcccccg tgcgcacgtg ctggggcact cgagcggcgc
    11041 gtcgatcgcg ctgcagcttg cggtggctca tcccggggcg gttcgctcgc tgatcctggt
    11101 cgagccggcc atcgtgggcg cggtggccgc tgccggcgag gtggctgccg cgctctcggg
    11161 gatcctcccg cgatacgcgg agggccggca tgccgaggca gtggaggcgc tgctctccta
    11221 ctgcaaccct ggcggttacc gcgaggcgct cgaggggcgg ctggcgccgg gttggttcga
    11281 gcgcgcggta gcgagttccg ccgtctattt cgagggcgag ctgccggcgc tggccgcctg
    11341 gtcggtcacc cgcgacgccg ccgcgcggat caccgtgccc acgctgctgg tgtgcggcgc
    11401 cgacagccca ccggtgttcc accagacgca agccctgctc gccgcctggc tgccgtgcgc
    11461 ccgcacggtc acgatccccg gcgcaggtca ccaggtgccg accgccgcgc cggaggcgct
    11521 ggcggaggcg atcgtctcgt tcctcacttg accttcaccg accgcccttc ccgctgcgcg
    11581 cgagcgagta ctacaacgac ttcctccggc ccaaggacat catccacgcg atcggcgggt
    11641 gcatctggcg ccgcccggac aaagcatgcg tgctgtcggt gttccgtgga caccacgaga
    11701 agccgcacga cgaagcggag gttgccctgc tcagggcgct cctcccgcat gtcgcccagg
    11761 ctctcgccat tcaggcgaag acggcggacc tcgagtcgag ggcacggtgg ggcgaggaga
    11821 gcctcgagca cctccaatcg gggtgatcgt cgcctccgcg gaagcccgcg tcctcttcgc
    11881 caaccgggcg gcccgccgtg tgctggatcg ccgcgacggg ctgcggctcg aaaacggtac
    11941 gctgcgtgcc gcgacgccca ccctgaacgc gtcgctgcgc tcctggttgc cgatgccgcg
    12001 acgaccagca acgggaacgg gacctcctcc gggggagccc tcccgatcgg acgcgcctcg
    12061 agcgccaggc tcctcgacgt agtggtgtgc ccgttccgct ccccggcgct cctcggacgc
    12121 ggcgccgcca tcgtgttcgt cagcgacccg gacgcccagc cccggtcgct gaacggcgcg
    12181 ctgcggcgcc tgtacggcct cacccatgcc gaggcacggg tcgcggagcg cctgtgttgc
    12241 ggggagagcc tggaggagat cagcgacgac acggccacga gcctcaacac cgtgcgaaca
    12301 cacgtgaagc gcgcgttgat gaagaccggc acgacgcgcc aggctcagct ggtggcgctg
    12361 gcgcttgggt tggcggccag ccccgacccc tcccaacctc aaccagaccg ctgacccgcc
    12421 gccgcctcct ccttcgccca tacaccaaac gggggatgcg aggcggctga cgcctctcaa
    12481 tactcggtac tcccgggggg atcggatgtg ggtggccatg gcccccgtgc aggcggacaa
    12541 gaccgctgag gcaatggccg agctgtccaa ggagattcgg tcggcagtgg gggatcggcc
    12601 actgaccgcg gaagaggtct ccgagtcgcg cgatcggcag atccggtcgc tcgcgggcag
    12661 gtgggagaca gctcacgacg tcttccatgc gctggacgag atcgtgaccc tcggtctccc
    12721 ggacgactac tacgcggcgt atccgaagaa gttgcgggcg gtcacgcctg agacggccaa
    12781 ttccgcggcg aagaagttca tccacccgga tcagctggtg tgggtggtgg taggcgatcg
    12841 ccagaagatc gagcccgtca tccgggcctt gaacctgggc gaggtgcagc ttctcgagcc
    12901 agagggtgcc gcgctgacag cgcgcgctca ggagggtgcg aaggttcgct gagcggcacg
    12961 cgccgggcgt gatcggggtg gccctctatc cctccggaaa ccacttcagc cggtcgcgga
    13021 gccccaccac ctcgccgatt acgacgatgg cgggggcctg gatgccggcg gtggcggcct
    13081 cgtcggcgat gcgcgagagc ggcgccgtga tcgtgcgctg ggcggtggtg gatccgtttt
    13141 cgatcaccgc cgccgggcac gagggggacc ggccgtgcgc cagcagtgag ccggcgacgt
    13201 cggccagact ggcgatgccc atgaagatga ccagcgtcgc ggagcttggg ccatagagcg
    13261 accagttcac cggtgtcggc ttcgattcgc actgctgccc ggtgaggaac accagctccg
    13321 aggtgtagcc gcggtgggtg gcggggatgc cggcgcaggc cggcaccgcg gtgccggcgc
    13381 tgacccctgg gacgatttcg taccggaccc cggcggccgc gagcacctcg gcctcctcgc
    13441 ccccgcgccc gaagacgaac gggtcgcccc ccttcagtcg gacgaccgtg cgcccggccc
    13501 gcgcttcctc gacgatcagc cggttgatcg cgtcctgcgc cagcacgtgc ggatcgtcgc
    13561 gctgtccggc cacgaacagg cgcacgcccg gccggcagtg ctcgagcagg gcccggtcca
    13621 cgagcgcgtc gaacacgacg gtgtcggcgc cgcgcagcag ttccaggccg cgcagcgtca
    13681 gcaggcgcgg gtcgcccggc ccggcgccca ccaggtagac cgtcccgacg ttcgaaccat
    13741 ccccaccgct catgtcagct ggccgccgtc ctaatgccac tcccactcga tgaagccggg
    13801 cttcacgtcg gtcatcgcgt tcacgatcag ctcggcgagg ccgccgtaga ggatgcggtg
    13861 cttcttccac agcccgtagt actcgatcgc gtcgcggaac tgccccttgc agttgctgca
    13921 cggcgcgcag atgtacttcg ggatcgacgg atcgaggcag tcggcgaagg cttcgagcgc
    13981 ctgctccacc ttcttgcgcc cggacacctg cagccgccag tcgcggaagt tgtgcccgct
    14041 catgatggcg aagccgctcc cgccgccgca gcagtagttg tcgaccccgt gcggcgccat
    14101 ctcgcggaac tgaggacaga tggcccgcag gatctcgcgc tgcggctcca ccacccccat
    14161 cagccggacg acgttgcacg ggtcgtggag cgtcaccggg aagtcgttgc gcgacgggtc
    14221 gagcttcagc cgcccgctcg ccacgatgtc gcggaacagc gtgaggcagc tctcgcgggg
    14281 gatgtcgaga tcgccggaga gcaccctgtc ggccaccacc gccagcgcct tgtgcgcgtg
    14341 gccgcactcg ccgaggacga tcttcctcac cttcagcttg cgcgccgcct ccgcgtgccg
    14401 gatcgccacg cgcgcgaact gcgcgtcgtc gtaccagagg ccgtagttga tcgagtcgta
    14461 gccggccagc tcggacgaca tcgtccacga gatgccggcg gcgttcagga tcaccgcgaa
    14521 ggcgcccggg ttctcgggcc acgccatgat ctcgccggcg ttgtggacga gcaggacgtc
    14581 ggcgccctcc acgtcccacg gcgtcttcac ctcgaggccc gtcttctcgc tcacgtcctc
    14641 gtcgacgaac tcgacgttgt cgcggacgac caccggcgtc atgcccgtcg acgagccggc
    14701 gctgagctgc agcatcgacc cgttgtcgtg cagctccttc ggcgcgattc ccagctcctg
    14761 gctgaagagc tttcgcagtt cgtgagccac caggccgttg tcggcgccga tcgggcaggc
    14821 ctgggcgcag cggcggcaca ggttgcagcg gtagctcagc tcggcgaggc gtgcgacgag
    14881 cggccagttg agggtgatgt cgccgtgcag ccaggccgcc agcaggccgc cgttcttcac
    14941 gtgcttgaag tagaggcgcc ggaggatctc ggcccggaag gtcggccggt agatctcctc
    15001 cccgccgctg gcctcgaaga cgtggcacgc ctccgcgcac gtctggcact tcgcgcagtg
    15061 ctccatcgtg agcaccagcg gctcgaggaa cgtccagttg ttctcgcgct cgaacagctt
    15121 gtcgagcccc gcgaggaagc gctccacgag cgcgcgctcc tcgtccggcc ccgccggcac
    15181 ggggaggttc aacgcgaggg tgccgtcgag cgccccgtcg aaccgctcgc gcgcctggtc
    15241 cgacagcggc gcgaacgggg gccgcgtccc ggaatcgtcg agcggcggcg gcagcggagc
    15301 gagcggcgcg cggtccacgc gcgtcacctc ctcgcggtcg cggcgggcga gatccgacag
    15361 ccgggtgggg ccgctcatcg ctgccctcgc cgcgggggcg tccccgccac gacgtcgcgc
    15421 caggtcttcg tcccgtccgc ctggacgtgg gacgccgacc aggtggggcg gtacgtgagg
    15481 tattcggtca tcttcgcgcg cacccgctcg ctcgtgggcc cgtcgtccca ccggacgttg
    15541 tgataggtga accacttccc cacgaagtgc gacatgtggg tcaacgggat gtaagcggcg
    15601 gtgagcgacg cgagtgcgag gccggcggcg agcgccgggt gcagcggcgc cgcggtgtcg
    15661 aagcgcgcca cccgcgccgc caccagcagg gatggcgcag gctccaggag gaagcccagg
    15721 cccgccagcg gcagcacgcc ggcgaagagc gcaaggttga agtagtcggc cggcgccgag
    15781 taggccgcca gcttcgggtc ggtcagccgc gcgtgcagca gcgccagcgc gccgccggcg
    15841 ctcaggacga cccccgcggt ccccaggagg tcgcacgccg cggtcagcgc gttcgccgcc
    15901 ccgtcgccga gcgggacacc ggccacgtcg agcagcgcgc ccgccgcgag cagcgcgcac
    15961 gcggccgcaa ccaggtagag tccgaggtgg aaggggtagg aacggtacca gagccggcgg
    16021 ttgtgctggc gaagtccggc cagcagcagc atctcgggaa gcatgtacgc cagctcgccc
    16081 agcaggtgcc tccgctgcgg ccgggtccac cactccagct cctcgtaccg cgagccgccg
    16141 tgtgcggccc gctcgggcgc ctcgtgggga acggggtaca gctcccagcg caagtgcacg
    16201 ggggcccgcg cgtagcgcac cgcccgtgcc gcggttgcgg cggcgaacac cccgaggccc
    16261 agccagatga gaaggaaggt aaggaccgtc acacgcaccc ggtcggcttc ggcaggccgg
    16321 ccaccttgca ggcgcccttc gcaggccccg acgggaacat ctcgtagatc tgcgcgagct
    16381 tgaagccggt ctccttgcag agcttccgga tcatcggggc catgccggcc tggaggtagt
    16441 actcgcggag gtagttcacc accttccact gctcctcgtt caggccgctg atgccctctg
    16501 tcgtggcgag atcctccgcc acctcccggt tccagatccc cgggtcctcg aggaacccgt
    16561 cgtcatccac cgcgtacgtc cgcccgttca acacgatcgt ggccataacc tgaaccttgg
    16621 gggcctgatc accaaatggg gccgtaccgg aagtgtgacg ttcttcacct ttccggtacg
    16681 gcccccgaga ctgtgacgtc tttcaccttc ccggtacggc cctcgccgaa ccctttccta
    16741 cgtcgtcttc gtcaccttct tcaacccgcg cggctggtcg gggtcgaacc cccgcaagcg
    16801 gctcaggtgg aaggcgagca gctgtcccgg caccaccgcc gcgatcggcg agagccactc
    16861 cggcagtccc tcgggcagcg gcagcggcgc ggccgccagc gaa
//
LOCUS       DAOPQX010000039        16762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4104_length_16762_cov_4.410187, whole genome shotgun sequence.
ACCESSION   DAOPQX010000039 DAOPQX010000000
VERSION     DAOPQX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16762
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4104_length_16762_cov_4.410187"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PKK95_03675"
     CDS             <1..1174
                     /locus_tag="PKK95_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17340.1"
                     /translation="VPDAHDPSKRHPPAMLTTDLALRYDPEYEKIARRFLENPDEFAL
                     AFAKAWYKLTHRDMGPISRYLGKLVPAEPQLWQDPVPPVTHPLIGEAEIADLKRQVLA
                     SGLSVSQLVTTAWASAASFRGTDKRGGANGARIRLAPQKDWEANNPPELAKVLRTLEG
                     IQGTFNEAHFGGVQVSLADLIVLGGCAAVEQAARDAGYDVEVPFAPGRTDALQEQTDV
                     ESFAVLEPAADGFRNYLAPGHAAPAEELLVERALMLTLTAPEMTVLVGGMRALGANAR
                     PSDIGVFTERPATLTNDFFVNLLDMSTEWRASGGGEVFEGRDRRTGERKWTGSRVDLI
                     FGANSELRALAEVYACDDAKEKFVRDFVAAWAKVMNLDRFDLKASEGAPAGVVESV"
     gene            1299..2159
                     /locus_tag="PKK95_03680"
     CDS             1299..2159
                     /locus_tag="PKK95_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17341.1"
                     /translation="MSRSDPFFGVQPGDASRRLTTAIDLLLALASAVVIGAWLTGRPL
                     FYSPVSPVMSPFTAFAVLIMVAVRQARLHLDTWPMALSLAMTGLVLGGNVSSIVMLAA
                     SPPGLWANFPDLVLTSTMTSVGLILFCCYDLVVALRETPRSAFIVDDLLIHLALVPGG
                     LSLLGYLLGNPAYLSVHGDPRVGISPLEMAFLGLYAAAAIVSNPRLFLWRFLAAGWTN
                     RVVFALLFANQFVAPLVAALLVSTPGTRGPGVELFVMLAGVVTTMSFLLLQARLARTA
                     AQETPAVGGR"
     gene            complement(2200..3021)
                     /locus_tag="PKK95_03685"
     CDS             complement(2200..3021)
                     /locus_tag="PKK95_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOC17342.1"
                     /translation="MTMKKVVAFVGAARKGYTYEATRVFLDRLEALGGVQTELVRLGD
                     YHLEPCRGCKACFSKGEEFCPLKDDRDVLLAKMKEADGVVFASPNYSFQVSALTKLFL
                     DRLGYGCHRPQCHGKAFTSIVVQGFFGGDKILKYFGLMGAALGFDVVKGSCHTALVPM
                     ADKEREKRDEALGRQAQRFRVQLGSETPHVPNLLEVVLFRLGRNSVRIELDEKEADYR
                     FYAERGWFNSPYFYPTRLGPVKRTVGRLADVYFARSIASKKAARRVHHQGAVPKA"
     gene            complement(3158..5608)
                     /locus_tag="PKK95_03690"
     CDS             complement(3158..5608)
                     /locus_tag="PKK95_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17343.1"
                     /translation="MDNLLTDLRYAVRVLRARPALAAAAVLSLAIGVGPVAALFSIVD
                     ALGFRPLPVRDPGGLLQVTTLTRGRPGQVSYPDFIDIRERSRLLTDLVVSGTQMFNLR
                     GDGQVPEVVVGATVSGDYFQTLGVRPSLGRPFLPDEDRTPGAAMVAMISDRLWRRRFG
                     ADPAILGKTIQLTRERCTIVGIVPAGFAGTDPILAPDVWVPTMAWPAFNRGVEETLQA
                     RERRELTLLARLAPGATLEQARAELSSLAGSLEEGRDAENRPAFVLEYEQAARRRPAA
                     MLGMFAFGVVALVLLVACANVAGLLLGRAETRSREVALRLAFGASRRRLVRQLLTESM
                     VLAFLAAIAGLALAALAVRLLPALVPTTGLPLTFDFRVDHRLFAATLLVALCAVPCFG
                     LAPALLASRRDLLPLLKGEGVVGGRARLPVRRLLVVVQISAAVVLLVATGFLLQSFFR
                     ARQIDPGFAQRPMVFATIVPGAVGYTQPQTREYYRLLRERVSSLPGVEAVSLARHIPL
                     NSLYGGGATVPVTVPGHEPGAGTEAPRFRYNTVTPGYFNTMGTPLLRGRDFSESDAAG
                     APGVAIVNETMARQFWPNGDAVGQSFEIPGTGDQASARRLQVVGVARDAKYMSLTESP
                     MPYFYLPLLQDPRGEVTFVARFSPGRTQAVAMLRREMLSVDPAVPILQIVTLDDHMHA
                     ALAVERAVTTLVAGIGALGLGLSLVGLYGVISFLVARRTRDIGIRMALGARQHHVVGQ
                     VLREGVWLALAGIAAGSAAAAAVMKVAARVIYGVNPKDPLVFLATAAVVMAVALTASW
                     LPARHAARVDPLAALRQE"
     gene            complement(5750..6859)
                     /locus_tag="PKK95_03695"
     CDS             complement(5750..6859)
                     /locus_tag="PKK95_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOC17344.1"
                     /translation="MSLSAFIRDHHEEIIVQFAVFARTLTPPGADMSEAELRDHAREI
                     LTAVVEDMSLAQTTDEQFEKSEGRGAAQSMRASGRLHADDRIHHGFTFRAVLAEFRAL
                     RATVLRLYAESGETDLRDVSRFNEAIDEAMTESMDRFAIQTDRFRDQFIGVLSHDLRT
                     PLGAITAGAALLAMPDDNPERRSWVASRIMNSAQRMERMIRDLLDVTHVRLGGAIPVR
                     RRPVDLQQLCDEAMIEIRTGQPDAVVRLQADGDLRGEWDPDRLAQVVSNLVGNAIQHG
                     GGTPVTLTAQEDGDSVTLAVHNGGPHIPPEVVPFVFEPLARGRHDNTRHNLGLGLFIA
                     RAIVSAHGGRIDVSSSADAGTTFTVTLPKASSAPE"
     gene            complement(7043..9979)
                     /locus_tag="PKK95_03700"
     CDS             complement(7043..9979)
                     /locus_tag="PKK95_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HOC17345.1"
                     /translation="MKLLSSSEAADPTDAVLAQLQASAIPLNGAGPFLLDETDAVWLV
                     ERGEVLVFAAEVCEGHQVGPRRYLWTTVAPGVLFGVEPANGRSSIGLVAVGSAATRAW
                     KVPRVAFEALGRHAESAGAVAALLDSFVTSASSAVASRTQPQFHALLEAHATATLRTA
                     QRASAKDGAIWVVHECGASSITDRPMLTADPSSPPLPIARGLWVAAGQDDVRIAALGT
                     REVLEDGRAWKGLDCFCTFLQRWAEEDAATADAAERERLVARARAEKQMRRDGLTGLA
                     DVLTPVAITAPAATQEDALVSACRAIGRTLGITFEPPPRWEVSSRARDPLGSICRTSR
                     VRYRRVALTGRWWRSAGEPLLAFRSEGREPVALLPGTRGYDLLDPMRGSRVHLAADDA
                     AALDRFGYQFYAPAPDRPLTGGAIARLGLDRTGRDLLFVVLTALAGSLLGLSLPIATG
                     AMFSSVIPNANAADARTLLAALVAMALGTALFEMTRAFALIRAEGRTNATMQALLVDR
                     LLSLPVPFFRNYSVGDLAQRASAVNRLRVLFSGAATTSLLSGLFSVVYLGLLLYYDLV
                     LALVAVSLLAVTVLATAACGVAAVRVRRRQQDAAGRIGGLVFQMVSGISKLRVAAAEG
                     RAFAVWCRRFREQKQLALVAGRYQNAIGVLNDVLPIVSLAVLFGAVQYRLKTGHDVHT
                     GTFIAFNAAFGTFFASGIAFSNTMIGLLEAVPIFERARPIFETAPEVDAARPDAGELT
                     GRVEGSHLSFRYSPDGPLVLQDISFRVEPGEFVAFVGPSGSGKSTLLRLLLGFESPET
                     GTIYYDGHDLGSVDVSAVRSQIGVVLQSSKLMAGDVFTNIVGSAPLTLDEAWEAAEMA
                     GFADDIRAMPMGMHTMISEGASTLSGGQRQRLLIARALVRRPRLIFFDEATSALDNRT
                     QEIVSRSLERMNATRLVIAHRLSTIRHADRIFVVDRGRLVQEGSFDELSRQPGLFAQL
                     IARQVA"
     gene            complement(9989..12181)
                     /locus_tag="PKK95_03705"
     CDS             complement(9989..12181)
                     /locus_tag="PKK95_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HOC17346.1"
                     /translation="MSNVAPGTADAGLRRKPRRVRTPTVLQMEAVECGAASLAIVLGY
                     YGRTVALEEVRVACGVSRDGSKAVNILRAARAYGLEAKGYRWEVETLRQATLPVIVFW
                     NFYHFLVVEGFGRDRVYVNDPAEGPRVLTNDEFDAGFTGIALSMTPGPNFERGGRRAS
                     LAAALRERVRGSGAAVALVALAGLALVIPGLLLPAFARLFVDKVLVANMDSWFKPLLA
                     GMVLTALVRLALSTLQRRYLLRLDARISITSSSAFLWHVLRLPIEFFNQRYGGEISAR
                     VGLNDEIAAFLSTRLASTAIDGLLVVCYAVLMAAYDPLLTLIGMAAAVLVVGATLFVN
                     RRRVDGNRRLLKEQAMAGATIMGGLSTIETLKATGAESDLFSRWAGYETKYINAQQQL
                     ALVTQLFLVIPPLLVSLTNALVLALGAIRVMEGALTMGTLVAFQTLMASFLTPVNNLV
                     SLASNLQEMEGSMGRVDDVLRYAVDPQTSTPEGEAEAGGGKLKGFVELDGVTFGYARL
                     DGPLIDRFDLKLTPGSRVALVGPSGCGKSTVAKLVTGLYEPWEGEIRFDGRPRRGLPR
                     ECLAGSVAIVDQDVALYEGTIRENLTLWDPSVPDAAIIAACKDACIHDDITARQGGYD
                     SYVAEGGANFSGGQRQRLEIARALVGNPRVVVLDEATSALDTVTEQMIDRNLRIRGCT
                     CIIVAHRLSTIRDADEIVVLDRGKVAERGTHEQLIAAGGLYAALAAES"
     gene            complement(12174..13418)
                     /locus_tag="PKK95_03710"
     CDS             complement(12174..13418)
                     /locus_tag="PKK95_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HOC17347.1"
                     /translation="MSELFRETAVDRLSSPEGLDTLMRITTPRAWVALLALVAVLGGA
                     TVWGVFGHAPDTMEGTGILLRRGGLFEVATEGAGRISELLVKPGDRVRAGQVVARLSQ
                     PELERTISQTEALIADLTRNGQQRGQFIGREKALGLSSLNERLRQIEHSADALREQVR
                     YLEHRRDAQAEALKAGLIAGDAYQGTVQELARVRDVLAGLDTERRQIAARKVELDNDA
                     GQRVFSLDQEVLAQQRQLELLQDRYAATAQVESPYSGLIVEQRADPGDLVAVGQVVFA
                     LELADEQLDSVVFVPMEGKQLKPGMQVQLSPAGVAWEDYGYMLGTVRSVSLSPASSAS
                     MNELLRNDTLVRQFADRGGAYMVTVDLFEDPGTPSGFKWTTRRGPDLQLGGGTLLGAR
                     FVLREERPITLVIPALRRWLGV"
     gene            complement(13411..15021)
                     /locus_tag="PKK95_03715"
     CDS             complement(13411..15021)
                     /locus_tag="PKK95_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC17348.1"
                     /translation="MRQRSCTSVIVAVAIGLAASAAAQEGHAPGPSPAAQPGAAASPS
                     RLEADLEALVAHALRLNLQLEGERMTPEIARTAVDAARAGFEPTVQFAPGFAIGSETI
                     QTAAGRVTSSTSAFSYANGLSGALPYSSLAVGGALPTGTRYSVAFDLARRSESPVRRQ
                     DHGVETDSQLTVSFAQPLLRGAGTSIARAEIRAAGLSVRAAARRFSRLEEETVAAVER
                     AYWALAQAEAAEAVERESLERAVALHTRNEQLVRLGLVAEVDLLTAQQAVAARQAAVT
                     EAARLRRDAAEELIFRVYGREAPDRLAMAGQIGVRALIVPPAVPPLEQAEAEALSRRP
                     DLIAAREDVAVGGIRVEVARSDLKPSLQLTGSYTAAVTDAARLRIWGTDRVGDVALTG
                     WQGGLVLTVPVGNGLGKAGYRQATLERSRQEAAVAALENDIRLQVRQAGRAIVEGTRR
                     LAQTEAALDLARRQYDAENRRLALGLTDSFRLLQVEQVVADASRAVTEARYAVASAVS
                     DYHLAIGGNVDRYAGAGASPNHPRGRDE"
     gene            complement(15018..16415)
                     /locus_tag="PKK95_03720"
     CDS             complement(15018..16415)
                     /locus_tag="PKK95_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17349.1"
                     /translation="MESHDIKRFVDWWKGDAAFRARFRADAARAVAEYGIRVDPDAVR
                     ELCEPAAGHTGPGEVKPCHAALDSYFRALEAQRAVYDDLDRLESAIGEPGFAAWRQRQ
                     RRRFASQAPDSWGRQNPHIMFAIELSDGCSIACPFCAGGAGRLKSVSRFTPANEALFR
                     GLLRRLSTLAACPAPSGLLYHYTEPLDNPDYEKFLQAYREELGELPQTTTAAWGRRPD
                     RTRRVLAMAREHGGRIQRFSVNSVEDFRSCMRTFAPEELRDVVIVPHYPEAGGIYYRT
                     GRARSLAEAVEGSVACVSGFLVNLAARTIQLVAPCIEPSAWPGGYRSLAASSFRTDDD
                     LDVFFERCREEVLDRILNDTTPIALRNDLHLSEDAGGTLELRSLYRSYRFEQGLQKAV
                     LRLAGERRSLGALVDELSVSHPEGEVFAFVQLLWDSGLLADSDVLAAEDVVTAPVLPR
                     SKLASAAAAHAGALP"
     gene            16506..>16762
                     /locus_tag="PKK95_03725"
     CDS             16506..>16762
                     /locus_tag="PKK95_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOC17350.1"
                     /translation="MDQLRHHVRVHAADGVAGRRSSLHHVASPALRLERAGADFLVLA
                     TNTMHKLVPRIEQRISIPVLHIADATARTIADMGLTTVGLLG"
BASE COUNT         2645 a         5833 c         5783 g         2501 t
ORIGIN      
        1 ggtgcccgac gcgcacgatc cgtcgaagcg gcacccgccg gcgatgctga ccaccgacct
       61 ggcgctccgc tacgatccgg aatacgaaaa gatcgcgcgc cgcttcctcg agaacccgga
      121 cgagttcgcg ctcgccttcg ccaaggcgtg gtacaagctg acgcaccgcg acatgggtcc
      181 catcagccgg tacctcggca agctggtgcc cgccgagccg cagctgtggc aggaccctgt
      241 gccgccggtc actcacccgc tgatcggtga ggccgagatt gccgacctca agcgccaggt
      301 gctggcctcg gggctgtcgg tttcgcagct ggtgacgacg gcctgggcgt cggcggcgag
      361 cttccgcggc accgacaagc gcggcggggc gaacggggcg cgcatccgcc tggcgccgca
      421 gaaggactgg gaggccaaca acccgccgga gcttgcgaag gtgctgcgca ccctcgaggg
      481 catccagggt acgttcaacg aggcgcactt cggcggagtg caggtgtcgc tggccgacct
      541 gatcgtgctg ggcggctgcg cggccgtcga gcaggcggcg cgcgacgccg gctacgacgt
      601 agaggtgccc ttcgccccgg ggcgcacgga tgccttgcag gagcagaccg acgtggagtc
      661 gttcgccgtg ctcgagccgg ccgccgacgg cttccgcaat tacctcgcgc cggggcacgc
      721 ggcgccggcc gaggagctgc tggtcgagcg ggcgctgatg ctgacactga ccgcgcccga
      781 gatgacggtg ctcgtgggcg ggatgcgcgc gctcggcgcg aacgcccggc cgtcggacat
      841 cggggtcttc accgagcggc ccgctacgct caccaacgac ttcttcgtca acctgctcga
      901 catgagcacc gagtggcgcg cctcgggggg cggcgaggtg ttcgagggac gcgaccgccg
      961 cacgggcgag cggaagtgga ccggcagccg cgtggacctg atcttcggcg ccaattccga
     1021 gctgcgcgcg ctcgcagagg tctacgcctg cgacgacgcc aaggagaagt tcgtgcgcga
     1081 cttcgtggcg gcgtgggcca aggtcatgaa cctcgaccgg ttcgacctca aggcgtcgga
     1141 gggggcgcca gccggcgtgg tggagtcggt ctgacggggg ccgtcccgaa gacgtagcgt
     1201 ttttcacagg ccgggggccg taccaagaac gtgaagtttt tcacgcctgc ggtacggccc
     1261 cgtcgtgtgt gaagtctttc acgcctgcgg tgcggccagt gtctcgaagc gaccctttct
     1321 tcggcgtcca gcctggtgac gccagccggc gtctcaccac cgccatcgat ctgctcctcg
     1381 ccctcgccag cgccgtcgtc atcggcgcgt ggctgaccgg gcgtccgctc ttctactcgc
     1441 cggtctcacc ggtcatgtcg ccgttcacgg ccttcgccgt cctcatcatg gtggcggtgc
     1501 ggcaggcgcg gctgcacctc gacacctggc cgatggcgct cagcctcgcg atgaccggcc
     1561 tcgtccttgg cggcaacgtg tcgtcaatcg tgatgctcgc ggcaagcccg cccggcctct
     1621 gggcgaactt ccccgacctc gtcctcacct cgacgatgac gtcggtcggc ttgatcctct
     1681 tctgctgcta cgacctcgtg gtcgccctcc gcgagacgcc gcggtcggcg ttcatcgtgg
     1741 acgatctcct gatccacctc gcgctggtgc cgggcggcct gagcctgctc ggctacctcc
     1801 tcgggaaccc ggcctacctg agcgtgcacg gcgacccgcg ggtgggcatc tcgccgctcg
     1861 agatggcgtt cctggggctc tacgcggcgg ccgcgatcgt ctccaacccc aggctcttcc
     1921 tctggcgctt cctcgcggcg ggctggacga acagggtcgt cttcgccctc ctcttcgcga
     1981 accagttcgt cgcgccgctg gtggccgcgc tactcgtctc gacgcccgga acgcggggcc
     2041 ccggggtcga gctcttcgtg atgctcgccg gcgtggtgac gacgatgtcg ttcctgttgt
     2101 tgcaggcgcg ccttgcccga accgcggccc aggagactcc tgcggtcgga gggcggtagc
     2161 ggtcgcagtc gcgcccggcg ctcgcttgcg tgcgtttcgt cacgctttcg gtacggcccc
     2221 ttggtggtga acgcgccgcg cagccttttt cgacgcgatc gatcttgcga aatacacgtc
     2281 cgccaggcgc cccaccgtcc tcttcacggg accgaggcgg gtgggataga agtagggcga
     2341 gttgaaccag ccgcgttccg cgtagaacct gtagtcggcc tccttctcgt ccagctcgat
     2401 tcggacgctg ttgcggccca gccggaacag cacgacctcg agcaggttcg ggacgtgcgg
     2461 ggtctcgctc cccagctgca cgcggaaccg ctgcgcctgc ctgccgagcg cctcatcccg
     2521 cttctcgcgc tccttgtcgg ccatcgggac cagggccgtg tggcagctgc ccttgacgac
     2581 gtcgaacccc aacgccgctc ccatcaggcc gaagtacttc aggatcttgt cgccgccgaa
     2641 gaagccctgc acgacgatgc tcgtgaacgc cttgccgtgg cactgggggc ggtggcaccc
     2701 gtagccgagc ctgtcgagga agagcttcgt gagagcagag acctggaacg agtagttcgg
     2761 cgacgcgaaa acgaccccgt cggcctcctt catcttggcg agcaggacgt cgcgatcgtc
     2821 cttgagcggg cagaactcct cgcctttcga gaagcacgcc ttgcagcccc ggcagggttc
     2881 caggtggtag tcgcccagcc gcaccagctc ggtctggacg ccgcccaaag cctcgagccg
     2941 gtcgaggaac actcgagtgg cctcgtaggt gtatcccttc cgcgccgctc cgacgaaagc
     3001 gacgaccttc ttcatggtca tggcgggcca cccaactggc gttcaccttg ccggatcgag
     3061 ggaagcggaa cgggcggacg aggcgtgtcg tggatgacgc cggcattgta gcagcaggcc
     3121 gtcgcgccgg atatcgggaa gtcgcgcgcc gcagcgccta ttcctgtcgc agcgccgcga
     3181 gcggatctac ccgggcggcg tgacgcgcgg gcagccacga ggcggtgagg gcgacggcca
     3241 tcacgaccgc cgcggtggcc aggaagacca gcggatcttt cgggttcacg ccgtagatga
     3301 cccgtgccgc caccttcatc acggcagcag ccgcggcaga gccggccgcg atgcctgcca
     3361 gcgccagcca cacgccctct cgaagcactt gccccaccac gtggtgctgc cgcgccccga
     3421 gggccatgcg gatgccgatg tcccgggtgc gccgcgcgac caggaacgag atgacgccgt
     3481 acaggccgac gagtgagagc ccgaggccca gcgcgccgat gccggccacc aacgtggtca
     3541 ccgcccgctc gacggcaagc gccgcatgca tgtggtcgtc gagggtcacg atttggagaa
     3601 tgggcacggc gggatcgaca gacagcatct cccgccgcag catcgccacg gcctgcgtgc
     3661 ggcccggcga gaacctcgcg acgaacgtca cctcgccgcg cggatcctgc agcagcggga
     3721 ggtagaagta gggcatgggc gattccgtga ggctcatgta cttggcgtcg cgcgccacac
     3781 ccacgacttg cagccgccgg gcggaggcct ggtcgcccgt acccgggatc tcgaaggact
     3841 gtcccaccgc gtcgccgttc ggccagaact gccgcgccat cgtctcgttg acgatggcga
     3901 cgcccggcgc ccctgccgcg tcgctttccg agaagtcgcg gccgcggagc aggggtgttc
     3961 ccatggtatt gaaatagcct ggcgtgacgg tgttgtagcg gaagcggggc gcctctgtcc
     4021 cggcccccgg ctcgtgcccc ggaaccgtga ccggcaccgt cgcccctccg ccgtagaggc
     4081 tgttgagcgg gatgtgccgg gcaagcgaga cggcctccac cccggggaga gaggacaccc
     4141 gctcgcgcag caggcggtag tactcgcgag tctgcggctg ggtgtacccc accgcccccg
     4201 gaacgatcgt ggcgaacacc atgggccgct gcgcgaaccc gggatcgatt tggcgcgcgc
     4261 ggaagaagct ctggagcagg aagccggttg ccacgagcag cacgaccgcg gctgagatct
     4321 ggacgacgac gagcaggcgc cgcaccggca gtcttgcccg gccgccgaca accccttcgc
     4381 ccttcagcag gggcagcaga tcgcgcctcg atgcgagcag ggcgggcgcg aggccgaagc
     4441 acggcacggc gcacagcgcg acgagcagcg tggcggcgaa gaggcggtgg tccacccgga
     4501 agtcgaaggt cagcggaagg ccggtggtgg gcacgagggc cggcagcagc cgaacggcga
     4561 gagcggccag ggcgagaccc gcgatggcgg ccaggaacgc gagcaccatg gattcggtga
     4621 ggagctggcg aaccagccgt cgccgcgaag cgccgaacgc gaggcgcagc gcgacctcgc
     4681 ggcttcgcgt ctcggcgcgc ccaagcagca gccccgccac gttggcgcac gcgaccagca
     4741 ggaccagggc gacaacgccg aaggcaaaca tgccgagcat ggcggcggga cgccgccgag
     4801 cggcctgttc gtactcgagg acgaacgccg ggcggttctc ggcgtcgcgt ccttcctcca
     4861 ggctgccggc gagggacgac agctcggcac gcgcctgctc gagcgtcgct cccggcgcca
     4921 gcctcgccag cagtgtcagc tctcgccgtt cccgggcttg aagcgtctcc tcgacaccgc
     4981 gattgaaggc cggccacgcc atcgtcggca cccagacatc cggagcgagg attgggtcgg
     5041 ttcctgcgaa cccggcggga acgatcccga cgatggtgca tcgctcccgg gtcagctgga
     5101 tggtcttgcc caggatcgcg gggtcggcac cgaaacgcct ccgccacagg cggtcgctga
     5161 tcatcgcgac catcgctgcg cccggcgtcc ggtcctcgtc cggcaggaag gggcggccga
     5221 gcgaggggcg aacccccagc gtctggaagt agtcgcccga gacggtggct ccgaccacga
     5281 cctcgggcac ctggccgtca ccccggaggt tgaacatctg cgtgccgctc acgaccaggt
     5341 cggtgagcag ccggctgcgc tcgcgaatgt cgatgaaatc agggtaggac acctggccgg
     5401 ggcgacctcg cgtgagggtc gtgacttgca gcaagccacc cggatctcgc acgggaagcg
     5461 gccggaatcc cagggcgtcg acgatgctga acagcgccgc gaccggcccg acgccgatgg
     5521 cgagcgagag cacggccgcc gccgcgaggg cgggacgggc tcgaaggacg cggactgcgt
     5581 agcgaaggtc ggtcagcagg ttgtccatcg gatggcgctc ccgctcgccg acaccccccg
     5641 cacggaggct tccgcaggga ggggggcgag gaaaagatcc ggagccctcc aatgcagtag
     5701 acgcagggat cagtgaagag gttcggcggg gcttcaagta gcgcggctgc tattccggcg
     5761 ccgatgacgc cttgggcagc gtcaccgtga acgtggtccc tgcgtccgca gacgaactca
     5821 catcaatgcg gccgccatgt gccgaaacga tcgcgcgcgc gatgaacagg ccgaggccga
     5881 gattgtgtct cgtgttgtca tggcggccgc gagcgagcgg ttcaaacacg aacggcacga
     5941 cctcaggcgg gatgtgcggg cctccgttgt ggaccgcgag cgtcaccgaa tcgccgtctt
     6001 cctgggcggt cagcgtgacc ggcgttccgc cgccgtgttg gatcgcgttg ccaacgagat
     6061 tcgacaccac ctgcgccagc cgatccgggt cccactcgcc ccgcaggtca ccgtccgcct
     6121 ggagcctcac gacggcgtct ggttgacctg tgcggatctc aatcatcgcc tcgtcgcaga
     6181 gctgctgcag atcgacgggc cgtcgcctga ccggaatcgc tccgcccaaa cgcacgtggg
     6241 tgacgtccag gagatcgcga atcatccgct ccatgcgctg cgcgctgttc atgatgcgcg
     6301 acgcgaccca gctgcgccgc tccggattgt cgtcgggcat ggccaggagc gcggcaccag
     6361 cggtgatggc tccgagtggg gtacgcagat cgtggctgag gacgccgatg aattgatctc
     6421 gaaaacggtc ggtctgaatc gcgaatcgat ccatcgactc cgtcatggcc tcgtcaatcg
     6481 cttcgttgaa tcgggacacg tcacggaggt ccgtctcccc gctttccgcg tacagccgca
     6541 gcaccgtcgc gcggagcgcg cgaaactccg ccagcaccgc ccgaaacgtg aacccatggt
     6601 ggatccgatc atccgcgtga agccggcccg atgctcgcat tgactgggcg gcgccgcgac
     6661 cctccgactt ttcgaactgt tcgtccgtcg tctgcgcgag gctcatgtcc tcgacgaccg
     6721 ccgtcaggat ctccctggcg tgatcccgca gttctgcttc ggacatgtcg gcgccaggcg
     6781 gcgtcagtgt tctggcgaag accgcgaact ggacgatgat ttcttcgtgg tggtcgcgga
     6841 tgaacgcgct caaggacatg ggtgcgctcc caacgtcggc cgggcggagg tctggcgtca
     6901 ccgtcgtcgc gtgcattcaa cgatagccca gtaccgactc gatcacgcgg tcaatctcga
     6961 agggcttccc tcatggatct cagcacggcg ggggagggcc aggcgtagaa gtcgtgtagg
     7021 tgcaacgaga taggtcgccg ccctacgcta cctggcgggc gatgagctgg gcgaagagcc
     7081 ccggctggcg cgacagctcg tcgaaggacc cttcctgtac cagccgtccc cggtccacca
     7141 cgaagatccg atcggcgtga cggatggtgg acagccggtg ggcgatcacg agtcgcgtgg
     7201 cattcatccg ctccaggctg cggctgacga tctcctgcgt gcggttgtcg agcgcgctcg
     7261 tcgcctcgtc gaagaagatg agacgcggcc ggcgcacgag cgccctcgca atcaacaggc
     7321 gctggcgttg cccgcctgag agcgtagagg ctccctcgct gatcatcgtg tgcatgccca
     7381 tcggcatggc gcggatgtcg tcggcaaacc cggccatctc ggccgcctcc caggcttcgt
     7441 cgagcgtcag cggcgccgag ccgacgatgt tggtgaagac gtcgcctgcc atcagcttgc
     7501 tgctctgcag gacgacgccg atctggcttc gcaccgccga gacatcgact gacccgaggt
     7561 cgtggccgtc gtagtagatg gtgccggtct cgggtgactc gaaaccgagc agcagccgca
     7621 gcagcgtcga cttgcccgat cccgacggcc caacgaaggc aacgaactcc ccgggctcga
     7681 cgcggaagct gatgtcctgg aggacgagcg gcccgtccgg actgtagcga aacgacaggt
     7741 ggcttccttc gacgcggccg gtcagctctc cggcgtcggg tcgtgctgcg tcgacttccg
     7801 gagccgtctc gaagattggc cgggcgcgct cgaagatcgg cacggcctcc agcagcccga
     7861 tcatcgtgtt gctgaacgcg atgccggaag cgaagaaggt cccgaaggcg gcgttgaagg
     7921 cgatgaaggt gccggtgtgc acgtcgtgtc ccgtcttcag gcggtattgg acggcgccga
     7981 agagcaccgc gagcgagacg atcggcagta cgtcgttcag tacgccgatc gcgttctggt
     8041 agcgtcccgc gacgagcgcc agttgcttct gctcgcgaaa gcggcggcac cacacggcaa
     8101 aggcgcgccc ctcggcagcc gcgacccgca gcttgctgat gcccgacacc atctggaaca
     8161 ccagaccgcc gatccggccg gcggcgtcct gctgccgccg ccgcacgcgc acggcggcga
     8221 cgccgcaagc cgcggtggcg aggactgtga cggcgagcag cgagacggca accaaggcca
     8281 ggacgaggtc gtagtagagc aacaggccca ggtacaccac tgagaacagg ccgctcagca
     8341 ggctggtcgt cgccgcgccg ctgaacagca cccgcagccg gttgaccgcg gacgcgcgct
     8401 gggccaggtc gccaaccgag tagttgcgga agaaaggcac cggcaggctc aacaagcggt
     8461 ccacgagcag cgcctgcatc gttgcgttgg ttcgtccttc ggcccggatg agcgcgaagg
     8521 cgcgggtcat ttcgaacaac gccgtgccga gggccatggc gacgagtgcc gccagcagcg
     8581 tgcgcgcatc ggccgcgttc gcattcggaa tcacgctgct gaacatcgcc cctgtcgcga
     8641 tggggagcga gagcccaagc aggctgccgg ccagcgccgt gagaaccacg aacagcaggt
     8701 cgcggccggt gcggtccagg ccaagcctgg caatcgcgcc cccggtgagc ggccggtcgg
     8761 gtgccggggc atagaactgg tagccgaacc tgtcgagagc ggccgcgtcg tccgctgcca
     8821 ggtggacacg actgccgcgc atcggatcga gaaggtcgta gccgcgggtg ccgggaagaa
     8881 gtgcgacagg ctcgcggcct tccgaccgaa acgcgagcag cggctcgccg gcgctgcgcc
     8941 accagcgccc ggtcagcgcc acgcgccggt atcgcacgcg cgaggttctg cagatcgacc
     9001 cgagcggatc gcgcgcacgg ctcgacacct cccacctggg cggcggctca aacgtgattc
     9061 ccagagtgcg gccgatcgcc cggcacgcgg agaccagggc atcttcctgc gtggccgccg
     9121 gcgcggtgat ggccacgggc gtgaggacgt cggccaaccc ggtcaggccg tcgcgtcgca
     9181 tctgcttctc ggcccgggcc ctggccacga gccgctcccg ctcggccgcg tctgccgtcg
     9241 cggcgtcctc ctcggcccag cgctggagga acgtgcagaa gcaatccagc cccttccacg
     9301 cccgtccatc ctcgagcacc tcgcgggtgc cgagggcggc gatgcggaca tcgtcctgcc
     9361 cggccgccac ccacaacccg cgcgcgattg gcagcggcgg agacgatggg tcggcggtga
     9421 gcatcggccg atcggtgatc gaggacgcgc cgcactcatg gacgacccag atggcgccgt
     9481 ctttcgcgct cgcccgctgc gcggtccgga gggtggcagt cgcgtgcgcc tcgagcagcg
     9541 cgtggaactg cggctgcgtg cgcgatgcga cagcgctcga agccgacgtc acgaacgagt
     9601 cgagcagggc tgccacggcc ccggccgact ccgcatggcg cccgagtgcc tcgaaggcca
     9661 cgcgcggaac cttccatgcc cgggtggccg cgctgccgac ggccaccagc ccgatcgacg
     9721 atcggccgtt cgccggctcg acgccgaaca ggacaccagg cgccacggtc gtccagaggt
     9781 aacgccgcgg tccgacctgg tgcccttcgc aaacctccgc ggcgaatacc agcacctcgc
     9841 cgcgctcgac aagccacacg gcgtcggtct cgtcgagaag gaagggcccg gcaccgttca
     9901 gcgggatggc ggaggcctgc aattgcgcca ggacggcatc cgtcgggtcg gcggcttcac
     9961 tggaggagag cagcttcatg cgggcaccct acgattcagc cgccagcgcg gcatacaggc
    10021 cgccggccgc gatgagctgt tcgtgcgtgc cgcgctcggc gaccttcccc cggtcgagca
    10081 ccacgatctc gtccgcatcg cgaatcgtcg acaggcggtg tgcgacgatg atgcacgtgc
    10141 agccgcgaat gcgcaggttc cggtcgatca tctgctcggt gacggtgtcg agcgcgctgg
    10201 tggcctcgtc cagcacgacg acgcgcgggt tgcccaccag cgcgcgcgcg atctcgaggc
    10261 gctgacgttg cccgccgctg aagttcgcgc ccccctctgc gacgtagctg tcgtagccgc
    10321 cctggcgcgc ggtgatgtcg tcgtggatgc aggcatcctt gcaggcggcg atgatcgcgg
    10381 catcggggac gctcggatcc cagagcgtga ggttctcccg aatcgtgccc tcatagaggg
    10441 cgacgtcctg gtcgacgatt gcgaccgagc ccgccaggca ttcccgcgga aggcctcggc
    10501 gaggacggcc gtcgaacctg atctcgccct cccacggttc gtagaggccg gtcacgagct
    10561 tggctaccgt cgacttcccg catcccgacg gtccgaccaa cgcaacacgc gaaccaggtg
    10621 tgagcttcag gtcgaagcgg tcgatgagcg gcccgtcgag gcgcgcgtac ccgaaggtga
    10681 ccccgtcgag ctcgacgaag cccttcagct tgccgccgcc agcctcggct tcgccctcgg
    10741 gggtgctcgt ctgcgggtcg acggcatacc gcaggacgtc gtccacgcgg cccatgctgc
    10801 cctccatctc ctggaggttg ctggccagcg acacgaggtt gttcacaggt gtcaggaagc
    10861 tggccatgag cgtctggaag gcgaccagcg tccccatcgt cagggcaccc tccatgaccc
    10921 ggatggcgcc cagtgcgaga acgagggcat tggtcagcga gacgagcagc ggcgggatca
    10981 cgaggaacaa ctgcgtgacg agggccagct gctgctgcgc attgatgtac ttcgtttcgt
    11041 agcccgccca gcgcgagaag aggtcagact cggcgccggt cgccttgagg gtttcgatcg
    11101 tggacaggcc tcccatgatc gtggcgcccg ccatcgcctg ctccttcagg agccgacggt
    11161 tgccgtccac gcgcctgcgg ttcacgaaca gcgttgcccc gacgacgagg accgcggccg
    11221 ccattccgat cagcgtcagc aggggatcgt aggcggccat caggactgcg tagcagacca
    11281 ccagcagccc gtcgatcgcg gtcgaagcga gccgggtgga caggaaggca gcgatctcgt
    11341 cgttcaggcc caccctggcg ctgatctccc cgccgtaccg ctggttgaag aactcgatcg
    11401 gcaggcggag cacgtgccag aggaacgcgc tcgatgacgt gatgctgatc ctggcgtcga
    11461 gccgcaggag gtaccggcgc tggagcgtcg agagagccag gcgcaccagg gccgtgagga
    11521 ccatgccggc cagcagcggc ttgaaccagc tgtccatgtt cgcgacgaga accttgtcca
    11581 cgaacaggcg cgcgaaggcc ggcagcagca ggccgggaat cacgagcgcc aggccggcga
    11641 gcgcaaccag ggccaccgcc gcgccagagc cgcgcacccg ctcgcgcaag gccgcagcca
    11701 ggctcgcccg gcgtccgccg cgctcgaagt tggggcccgg ggtcatgctg agcgcgatgc
    11761 ccgtgaaacc ggcgtcgaac tcgtcgttgg tcagaacacg cggaccttcg gccgggtcgt
    11821 tcacgtagac ccggtcgcgg ccgaacccct ccacgacaag gaagtgatag aagttccaga
    11881 agacgatgac cgggagcgtt gcctgcctca gggtctcgac ctcccatcgg taccccttgg
    11941 cttcgaggcc gtacgcgcgc gccgcccgca ggatgttcac cgccttcgag ccgtcgcgcg
    12001 agacgccgca ggcgacgcgc acttcctcga gcgccaccgt gcggccgtag tagccgagca
    12061 cgatggcgag cgaagcggcg ccgcactcga ccgcctccat ctgcaggacg gtcggcgtgc
    12121 gtacccgccg cggcttgcgg cggagcccgg cgtcggccgt gccgggcgcg acgttagaca
    12181 ccgagccacc tcctgagcgc ggggatcacc agggtgatcg ggcgctcttc ccgcaggacg
    12241 aagcgcgcgc caagcagcgt cccgccgcca agctgcagat cgggtccccg tcgtgtcgtc
    12301 cacttgaagc ccgagggcgt gccggggtcc tcgaacaggt ccaccgtcac catgtaggcg
    12361 ccgccccgat cggcgaactg gcgcacgagg gtgtcgttgc gcagcagctc gttcatcgat
    12421 gcggagctgg cggggctcaa ggaaacggat cgcaccgttc ccaacatgta gccgtagtct
    12481 tcccacgcca cgccggcggg cgagagttgc acctgcattc ccggcttcag ctgctttcct
    12541 tccatcggta cgaacaccac cgagtcgagc tgttcgtcgg caagttccag ggcgaacacg
    12601 acctgcccca cggccaccag gtcgcccggg tctgcgcgct gttccacgat cagcccgctg
    12661 tacgggctct cgacctgcgc ggtggccgcg tagcgatcct gcagcagctc cagctggcgc
    12721 tgctgggcga gcacctcctg gtcgagcgag aagacgcgtt ggccagcgtc gttgtccagc
    12781 tccacctttc gagccgcgat ctggcgccgc tcggtgtcca gccccgccag gacgtcccgg
    12841 acgcgcgcca gttcctggac ggtgccctgg taagcgtcgc cggcgatcag gcctgccttc
    12901 aaggcctcgg cctgcgcgtc gcgccggtgt tcgaggtatc gcacctgctc ccggagggcg
    12961 tcggccgagt gctcgatctg gcgcagccgc tcgttcaggc tggagaggcc gagggccttc
    13021 tcgcggccga tgaactggcc ccgctgttgg ccgttccgcg tgaggtcggc aatcagggct
    13081 tccgtctggc tgatcgtgcg ttcgagttcc ggctggctca ggcgcgcgac cacctgtccc
    13141 gcacgcactc gatcgcctgg cttgaccagc agctcgctga tgcggccggc gccttcggtc
    13201 gcaacctcga acaggccgcc gcgccgcagc aggatcccgg tgccctccat cgtgtcgggg
    13261 gcatgcccga agacgcccca cacggtcgcg ccgcccagca ccgcgacaag cgccaggagg
    13321 gcgacccagg cgcgtggcgt cgtgatccgc atgagcgtgt cgagcccctc cgggctcgag
    13381 agccgatcga cggcggtctc gcgaaacagc tcactcatcg cgacctctcg ggtggttcgg
    13441 ggatgccccg gcgccggcgt aacggtcgac attccctcca atggcgaggt gatagtctga
    13501 gactgccgac gccaccgcat accgggcttc ggtcaccgcc cggcttgcgt cggcaaccac
    13561 ctgctcgacc tgcagcaggc ggaacgagtc ggtcagcccc agggccaggc ggcggttctc
    13621 ggcgtcgtac tgacggcggg ccaggtccag cgccgcctcg gtttgggcca ggcgccgcgt
    13681 tccttcgacg atcgcccgtc ccgcctggcg gacctgcagt cgtatgtcgt tttcgagggc
    13741 cgctacggcg gcctcctgcc gcgaccgctc gagcgtggcc tgccggtacc ccgccttccc
    13801 gagcccgttg cccacgggga ccgtcagcac gagtccgccc tgccagcccg tcagcgcgac
    13861 gtccccaacg cggtcggtgc cccatatccg caggcgggcg gcgtcggtca cggccgccgt
    13921 gtacgagccg gtgagctgca ggctgggctt gaggtcgcta cgcgccactt cgacgcggat
    13981 cccgccgacg gccacgtcct cgcgggcggc gatcaagtcg ggccggcgcg agagcgcctc
    14041 ggcctcggcc tgctcgagtg gcggaacggc cggcggcaca atgagggcgc ggacgccaat
    14101 ctgtccggcc atcgccagcc tgtcgggcgc ctcgcggccg tacacccgga agatcagttc
    14161 ttccgccgcg tcacgacgca gccgcgccgc ctcggtgacg gcggcctgac gcgcggcaac
    14221 cgcctgctga gcggtgagca gatccacctc cgccacgagc ccgaggcgca ccaactgctc
    14281 gttacgggta tgcaaggcca cggcgcgttc gagcgattcg cgctcgacgg cctctgcggc
    14341 ctcggcctgc gccaacgccc agtacgccct ctcgaccgcg gcaacggtct cctcttcgag
    14401 gcggctgaag cggcgtgccg ctgcccggac agacaggccg gcagcccgga tctcggcgcg
    14461 cgcgatcgag gtgcccgctc ccctgagcag cggctgggcg aacgagacgg tcagctggct
    14521 gtcggtttcc acgccgtggt cctgccggcg caccgggctc tcactcctcc gagcgagatc
    14581 gaacgcaacg ctgtacctcg tgccggtcgg cagcgcgccc ccgaccgcaa ggctgctgta
    14641 cggcaaggca ccggacaacc cgtttgcgta cgaaaacgcg ctcgtcgagc tggtcacgcg
    14701 accggctgcc gtctggatcg tctcgctgcc gatcgcgaag ccaggagcga actggaccgt
    14761 gggctcgaat cccgcgcggg cggcgtcgac ggcggtgcgg gcgatctcgg gcgtcatgcg
    14821 ctcgccttcc agctgcaggt tgaggcgaag tgcatgcgcg acgagcgcct ccaggtcagc
    14881 ctcgaggcgc gacggtgacg cggcggcgcc cggttgcgcc gccggtgacg gtcccggcgc
    14941 gtgaccctcc tgggctgcgg ccgacgcagc aaggccgatg gcgacagcca cgatgaccga
    15001 cgtgcaggac cgctgtctca tggcaatgcg ccggcgtgtg ctgcagcggc cgaagcgagc
    15061 ttgctgcgcg ggagaaccgg cgcggtcacc acgtcctctg cggcgagcac gtcggaatcg
    15121 gcgaggaggc cgctgtccca gagcagctgg acgaaggcaa acacctcacc ctccggatgc
    15181 gagaccgaga gttcgtccac gagggcgcca agggagcgtc gttcgccggc cagccgcaga
    15241 accgccttct gcagtccctg ctcgaagcgg tacgatctgt agaggctgcg aagctccaac
    15301 gttccgccgg cgtcctcgct caagtggagg tcgttccgca gtgcgatggg agtcgtgtcg
    15361 ttgaggatgc ggtccagcac ttcctcgcgg caccgctcga agaagacgtc gaggtcgtcg
    15421 tccgtccgaa acgaactcgc ggcgagcgag cggtagccgc cgggccacgc agacggctcg
    15481 atgcacgggg ccaccagctg tatcgtccgc gcggcgaggt tcacgaggaa gcccgacacg
    15541 cacgccaccg aaccttcgac cgcttcggcc agcgacctcg cgcggccggt ccggtaatag
    15601 atgccgcccg cctcggggta gtgcggcacg atgacgacgt cgcgcagttc ctcgggcgcg
    15661 aaggttcgca tgcaggagcg gaaatcctcg acgctgttca ccgaaaaccg ctggatgcgg
    15721 cctccgtgct cgcgcgccat cgccagcacg cggcgcgtgc ggtcggggcg ccggccccag
    15781 gcggcggtcg tggtctgcgg cagctctccg agttcctcgc gataggcctg gaggaacttc
    15841 tcgtagtcag ggttgtcgag cggctcggtg tagtgataga gcaggcccga cggtgcggga
    15901 caggcggcca gcgtcgagag gcggcggagc agtccgcgga agagcgcttc gtttgccggc
    15961 gtgaagcggc tgacgctctt caggcggccc gcgccgccgg cacagaacgg gcaggcaatc
    16021 gaacagccgt ccgacagctc gatcgcgaac atgatgtgcg ggttctgccg gccccacgag
    16081 tcgggcgcct ggctcgcaaa gcggcggcgc tggcgctgcc gccacgcggc gaaccccggc
    16141 tcgccaatgg ccgattcgag acgatcgaga tcgtcgtaga cggcccgctg cgcctccaga
    16201 gcgcggaagt acgaatcgag cgccgcgtgg cacggcttca cctcgccagg cccagtgtgg
    16261 ccggccgccg gctcgcacag ctcacgaacc gcatcgggat ccacgcggat gccgtactcg
    16321 gcaaccgcgc gggcagcgtc cgcacgaaag cgggcgcgga acgcggcgtc gcccttccac
    16381 cagtccacga agcgcttgat gtcgtggctt tccatgcctc gccgtccgtc cggctacaac
    16441 tccagggaga cgcgtttgta cgcgacgggg cgtccaaggc ccgggggtgg acagggagcg
    16501 ttcgagtgga ccagctgcgc caccacgtcc gcgttcatgc ggcggatggt gtcgcaggcc
    16561 ggcgaagcag tcttcatcac gtcgccagtc cggcgctgcg cctggagcgg gcaggggcgg
    16621 actttctggt cctggccacc aacaccatgc acaagctggt cccccgcatc gagcagcgca
    16681 tctccatccc tgtcctgcat atcgcggacg ccacggcccg caccatcgcg gacatgggac
    16741 tgaccaccgt cggactgctg gg
//
LOCUS       DAOPQX010000040        16679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4123_length_16679_cov_4.895212, whole genome shotgun sequence.
ACCESSION   DAOPQX010000040 DAOPQX010000000
VERSION     DAOPQX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16679
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4123_length_16679_cov_4.895212"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="PKK95_03730"
     CDS             complement(<1..1403)
                     /locus_tag="PKK95_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOC17351.1"
                     /translation="MTTKTIYEPKYVSSHALVIGINEYDHAPRLGYARNDAEAFAQAL
                     CGKFKFPESNIALLTDGAATRNEILRVFMKYAQDGSIAGDERIVVFFAGHGYTVTGRR
                     GETGFLVPVDGNVADLSTLIRWDDLTRNADLIPAKHMFFIMDACYGGLALTRMALAAG
                     SMRFLRDMLQRFSRQVLTAGKADETVADANGPRPGHSIFTGHLLDALEGGAASEGVIT
                     ASGVMAYVYEKVARDPYSRQTPHYGFFDGDGDFIFAADGLKGPVGEEKGATDVPIKVV
                     GPTAPVLAEEAVAGTLKELLSTPSQQIRLDDFVSAHVRRVLQLVNVKRFPVQGVSSSK
                     EEFASRLAGYEAAISDVATMTVLMGRWCTREQLPILDKALARLAESDKGSAGTTLWLR
                     LGWYPILYLMYAGGVAALSADNYSTLATILKAPVHSEPHDGGELKPLTVAVIEELTEI
                     VDAFKSLPGHERHYVPR"
     gene            2183..2467
                     /locus_tag="PKK95_03735"
     CDS             2183..2467
                     /locus_tag="PKK95_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC17352.1"
                     /translation="MGNAADFLELSAEESALVETKLALSESVRERRSKQGLSQAELAK
                     RLKSSQSRVAKIEAADPSVSTDLVLRALFALGATRNDVASAIRKGRPVAA"
     gene            3020..3457
                     /locus_tag="PKK95_03740"
     CDS             3020..3457
                     /locus_tag="PKK95_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17353.1"
                     /translation="MSVSLTWGLLSAIVVTAVLLLSVSPLEAQGTQASPPLPASLERI
                     RKELAKPPARRLKLDGIQTSVPVFRATVEREDVPSFKALLEKEFRLNDFQRQSQEWSS
                     KCCGLDLIGLVKGVDKYLKKREARKIREQIARELAQIEAASKK"
     gene            complement(3541..6219)
                     /locus_tag="PKK95_03745"
     CDS             complement(3541..6219)
                     /locus_tag="PKK95_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17354.1"
                     /translation="MRRRDFLRAGLAGGAVLGGLPALSRGERAASVALIVDPEDPVAS
                     SAAGRWAIGELRSALSGAGVAMLDRHDLRDVPSDAACIVAGGARSPMVQSALAGSGLT
                     PPDVPESLVLAAAKLSRRPATVACGPDARGLAYALTELADRARHAARPHAALHVETPV
                     VERPANRVRSVMRQFTSEPLDKPWFYDRGMWPAYLDMLATHRFNRFHLAFGLGYDFLR
                     HVDDAYFLFAYPFLLEVPGYRVRAANLADEERDRNLDTLRFIARQTVARGIDFQLGIW
                     MHGYVWHDSPRAQHTIEGLTPETHAAYCRDALAALLRACPEISSVALRTHGESGVPEG
                     NYAFWRTIFDGVARCGRRVEIDLHAKGLDTRMIDAALDTGMPVNVALKGWAEHQGLPY
                     HQAAIREHEMPVEGRSGQGLMTLSEGSRSITRYGYGDLLRDDRRYTVTHRVFSGTQRL
                     LLWGDPAAAAAYSRAFQFCDSAGTDLMEPVTCRGRRGTGKAGGSRIGYADPQLDPQWD
                     WQKYGYWYRVWGRHLYNPETPPAVWQRHFGGSPAGRALESSLARTSRILPLVSISHMP
                     SAACDAYWPEVYWNQPMVRESDPNPYTDSPSPKTFQHVSALDPQLFSSMSDCAEELLQ
                     RRPSGKYSSVEVSQWLADLAQRGEEDLRRAGPLRPLDWRRTGIDIEILIGLGRFFAAK
                     LRSGVLYSIHERTGDRPALEAAIRTYKAARAAWASVAACAKGVYADDLSASDKISNRG
                     AWADRLPLIDADIAQMEERLAGASRSTDPAVAAAVAAVLGRPRREPPACNHEPPRRFR
                     PGEELPLEIVHRGRVTPSSAVLYYRHVNQAERFQTVTMTAGSGSHRAVVPAEYTDSPY
                     PLMYYFQLTHGRGSVTLYPGFSGDLLNQPYFVVQRG"
     gene            complement(6289..6543)
                     /locus_tag="PKK95_03750"
     CDS             complement(6289..6543)
                     /locus_tag="PKK95_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17355.1"
                     /translation="MARGWESKSVESQQDEHERGTKRVAGPRTPEQRERAARRLTLEL
                     QRAKARADLARAVKPGHRAMLEQAISALDEQLRGMDGEPR"
     gene            6726..8204
                     /gene="gltX"
                     /locus_tag="PKK95_03755"
     CDS             6726..8204
                     /gene="gltX"
                     /locus_tag="PKK95_03755"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOC17356.1"
                     /translation="MSSPRVRFAPSPTGYLHVGGARTALFNWLYARHNGGTFVLRIED
                     TDLERSSAEMVTGILDGMRWLGLDWDEGPEVGGAHGPYFQSERLERYRSAAESLVASG
                     HAYYCYCSPEELKAKRAAAEAAGGDAAAAWQYDRTCANLPASEIARREAAAMPRAIRF
                     RMPPGTTAYDDVVHGRIEFDNAVIEDFVVLRSDRHPTYHLSVVVDDIDMRITDIVRGD
                     DHISNTPKQVLLYQAFDAALPRFAHVPLILGPDKRRLSKRHGATSVMEYARQGFLPEA
                     MFNFLALLGWSPGDDREFFTRGELIEAFTLEGISSSNAVFNPEKLDWFNSQHLARLSG
                     DELTGRLEPLLRERGWWRESLRGPERPWFHQVLEVLKPRARKVADLVEQGRYFFEAPT
                     GYDAGAVKKHLSDPALAGPLLAWREAILAVEPFDPPSIEQALRATAAARGVKAAALIH
                     ATRIALTGQAVSPSLFEVAALVGREASATRIEALHRFLEAGK"
     gene            8434..8655
                     /locus_tag="PKK95_03760"
     CDS             8434..8655
                     /locus_tag="PKK95_03760"
                     /inference="COORDINATES: protein motif:HMM:NF022150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HOC17357.1"
                     /translation="MAKEDRGFASMDRAKQREIASKGGKAAHQKGTAHEWTSEEAREA
                     GRKGGMASHRRRQQSGGQASESSESGSQR"
     gene            complement(8751..11180)
                     /locus_tag="PKK95_03765"
     CDS             complement(8751..11180)
                     /locus_tag="PKK95_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17358.1"
                     /translation="MCRRNAFHARLDFRKKQVPRGFTAVAACTLALGIGANTAVFSLV
                     NAVLLRPLPYRQSDRLAVIWERRLDSDNPANVVSPANFLRWQERSRTFSQMAAIATST
                     ATLTAAGEPEELQGQAVNASLFPMLGAMPALGRTFLESEDRPGGDAVVVLSDRLWRRR
                     FNADPAIVGRGITLGGEAVTVVGVMPPGFSILSPASEFWTPIAFPVEARTPRGRYLTV
                     VGRLRDGVSLEDARSEFEAIAASLRAELPDFDEGWGVRVVSLNEQLLGEIRPVLLVLF
                     GAVGFVLLLACVNVANLSLNRGLGRERELGLRTALGAGRWRIVQQLLTEGVVLAAIGG
                     SLAVLLAWWSLDYLLAAAVHLLSLPRPADEITLDGGVLAFASALSLGTVLVFALVPAL
                     SLSRMRIQGALREGARAGKESRSHRHLRRVLAAAQLALAFTLLVAAGLMIRSIGSLLD
                     VDPGFRPDHVLTMRVLLQQSRYEEPASRAALFDRLIEELSRVPGVRSAGVSSGFPFRG
                     VPIATGFSIEGRPFPGEANLPSADIRIIAGRYFETMRIPLRRGRLFEARDGEAGRGAA
                     IVNEALVRQIFPDENPLGRWLRVRLGENGSVEAQIVGVVGDVRHEALDRDVRPMIYLT
                     NRQMAFPLMGVVIRTASAPDAMALQALGQLRRLDPELPVSQVNTMDEVVNETIAQRRF
                     VMMLLAAFAGVALLIASVGVYGVLAYSATQRIPEIGVRLALGAPPAGLVRQFLRESAA
                     LVAAGLVAGAGIGLALSGLLGRLLYGVTPRDPVTFASAAALLGLIAMLAAFLPAWRAS
                     RVDPVTALRRE"
     gene            11187..12272
                     /locus_tag="PKK95_03770"
     CDS             11187..12272
                     /locus_tag="PKK95_03770"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOC17359.1"
                     /translation="MADSAPGFAIAPPIEPMLARLADTLPAGDFIYEPKWDGFRAIVF
                     RGGAEVFIQSRDLRPLDRYFPELHAEFLAALPEGCVLDGEIVIAAPSGLDFDALQMRL
                     HPAASRVAKLAGETPASFVAFDLLAESGRDLRALPQRERRDRLERLLGAATPPIHLTP
                     ATLDLGTATEWLARFEGAGLDGVVAKLSELQYQPGKRAMVKVKHARTADCVVGGFRWH
                     KSGHDRVGSLLLGLYDSGGTLHHVGVTSAFTMATRKALALELAPLREDALKDHPWREW
                     AEGGADAAARMPGAQSRWSAGKDLSWEPLRIERVCEVKYDHLQGDRFRHATQFLRWRP
                     DKRPRDCRYDQLEVTPPYELERVFGAA"
     gene            complement(12311..12865)
                     /locus_tag="PKK95_03775"
     CDS             complement(12311..12865)
                     /locus_tag="PKK95_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17360.1"
                     /translation="MRSLLHFIYSIVMRRNLFVLLLPAALLAAEGCFAPPTLNVPSSP
                     RTEVRIGSISFTITGASLNRDFAPRVLDPGPDGGGTLGGLVNIRVHNAGQDDHRFNAA
                     AVVYDERDGNHPVTAVPRIAGQPLVWDGTIAPGGTVDLEVAIGNGPYVEIGARVSVVL
                     MFVRHDGDTGTLRTPPIEVGALRS"
     gene            complement(12862..13878)
                     /gene="ligD"
                     /locus_tag="PKK95_03780"
     CDS             complement(12862..13878)
                     /gene="ligD"
                     /locus_tag="PKK95_03780"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HOC17361.1"
                     /translation="MAPQETVKVDGVEVAVTNPGKVLFPEPGHTKLDLVRYYLAVAEG
                     ALRGSGGRPNVLVRYPNGVDGEFFYQKRAPASRPSWVEVVTLRFPSGRSAEEVVPRGA
                     ATLAWMANLACLELHPHPVRAEDVDHPDELRVDLDPVPGVRWPQVREVAGIVRATLGD
                     FGLTGWPKTSGSRGIHVAVRIAPRWTFDQVRQAALAFAREVERRAPALATSKWWKEER
                     HGVFLDYNQNAKDRTVAGAYSVRPKPDARVSAPLSWDEVGDCDPADFTLKTMPSRFAA
                     VGDRLAGIDDHPCSLDALLELSARQQRDGQGDAPWPPHYRKQPDEPARVQPSRRRSSR
                     GARS"
     gene            13925..15115
                     /locus_tag="PKK95_03785"
     CDS             13925..15115
                     /locus_tag="PKK95_03785"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOC17362.1"
                     /translation="MSGREVVIVGAARTPIGSFLGSLASVPAPRLGAVAIRCALQRAG
                     VDPAAVDEVVMGNVLQAGVGQAPARQAAIFAGLPPTTPCWTMNKVCGSGLKAVISGAQ
                     AIALGDAEVVVAGGMESMSNAPHYLRVRAGQRLGHASLVDGLVFDGLTDAYDSRHMGE
                     WAEVTAAARAITRQMQDEFAAESTRRAVAAQKQGLFAAEIASVEIEGRKGEKTVVSED
                     EGPKTARPDKIASLKPVFKKDGTITAANASSINDGAAAVVLMSAERAAGEGRPVLARI
                     TAWGGAARTPGEYTIAPTDAIRATLSRAGLGIDDIDLWEINEAFAVVSLANNMLLGLD
                     PAKVNIRGGAVVLGHPIGASGARILVTLLHALRDERKKRGVASLCIGGGEAVALVVER
                     PSAG"
     gene            15117..15824
                     /locus_tag="PKK95_03790"
     CDS             15117..15824
                     /locus_tag="PKK95_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOC17363.1"
                     /translation="MQTLLIACRTLESEIALAGREAGIARPTRWVEPGLDARPDRLRD
                     LLRDELHRVSCENASRAASKGASTGRVLLAFGYCGHALLGLHAASFELVFPRADDCIS
                     LLLGSAERREAVAGETPTYFLTEGWLEHETNVWSDYQRAVATMGAERAKQVMAMLLAP
                     YERLGVIDTGACPLARVTERTRPIAETFGLRHEVIPGSTRYLRQLLAGPWGDEFVTIP
                     PGGTVTLDHLLRQDRTP"
     gene            15821..>16679
                     /locus_tag="PKK95_03795"
     CDS             15821..>16679
                     /locus_tag="PKK95_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOC17364.1"
                     /translation="MIHRILCPLDLSDFSKRAFEHGLVLARWYGAKVVALHVFAGFMP
                     PGDESTYPAWLRRVPEARKEIDRELHEQVSSPLAEGMDVPLVLREGDPVAGILAEAKA
                     MAADLVVMSTHGRSGFDRLTLGSVAYKVLRKSPCPVLIVPAQQARQGEFQGYRRIICA
                     TDFSPDSGHALDFALSIAERAGSRLTIAHVVEVAENPEVLEGDSPLAVVRRERIEKAH
                     ARLRELVAAREGQEPAVDETVRIGRPYVELLALAKATEADLLVLGVRGRGAIDLTLFG
                     STTAQVVRRA"
BASE COUNT         2643 a         5779 c         5751 g         2506 t
ORIGIN      
        1 cttcgcggca catagtgacg ttcatggccc ggcagcgact tgaaggcgtc gactatctcc
       61 gtgagttcct cgatcacggc aacggtcaac ggcttcagct caccgccgtc atgcggttcc
      121 gaatgaaccg gagccttaag gatggtcgcc aaggtgctgt agttgtccgc agacagggcg
      181 gctactcctc ccgcgtacat cagatagaga atcggatacc aaccaaggcg taaccacagg
      241 gttgtgccag cagacccctt gtcactctcc gccaacctcg cgagcgcctt gtcgagaatc
      301 gggagttgct cacgggtgca ccaccgcccc atcagaacgg tcatcgtggc gacgtcggag
      361 attgctgcct cgtacccagc aagacgagag gcgaattctt ccttactact gctcactccc
      421 tggacaggaa atcgtttcac attgacgagt tggagtactc tgcgaacatg cgctgacaca
      481 aagtcatcca gccgaatctg ctgcgacggc gtcgacagca actccttgag cgtccccgct
      541 actgcctcct cagccaatac cggagcggtc ggcccaacca ccttgatggg gacatctgtc
      601 gcgccctttt cttcgcccac cggtcccttg agtccgtctg ccgcaaagat gaagtcgcca
      661 tcgccgtcaa agaacccata gtggggtgtc tgccgcgagt atgggtctct ggcaaccttc
      721 tcgtagacgt acgccatcac cccactcgcc gttatgacgc cttcgctcgc cgcaccacct
      781 tccaacgcat cgagaagatg tcccgtgaag atcgagtgac cgggccgagg accgtttgcg
      841 tcagcgacag tctcatccgc cttgccggcg gtcagtacct gtcgtgagaa ccgctgcaac
      901 atgtccctga ggaatcgcat actcccggcc gccaaagcca ttctcgttag cgcgagtcct
      961 ccatagcacg cgtccatgat gaagaacatg tgcttggccg ggatcaggtc ggcgttgcgc
     1021 gtgaggtcgt cccaccttat gagggtggag aggtcggcca cgttgccgtc aactggcact
     1081 aggaagccgg tctcgccgcg acggcctgtc accgtgtatc cgtggcccgc gaagaacacg
     1141 acgatccgtt catcgcctgc aatactcccg tcttgggcgt acttcatgaa cacgcgaaga
     1201 atttcgttgc gcgtcgcggc gccgtcggtc aggagagcga tgttgctctc agggaacttg
     1261 aatttaccgc aaagagcttg cgcgaatgct tcggcatcgt tccgcgcgta tccgagccgt
     1321 ggggcgtggt cgtactcgtt gattccgatc acgagcgcat ggctcgacac gtatttgggt
     1381 tcgtaaatcg tcttcgtcgt catggatgta gtgcggacgg agttccgatc gagcaggtcg
     1441 acctcgctgg tgcgcctgct ccgtgtacgg aacatactac gcggttccag ccactttgac
     1501 tgaccgcctc gaggacctgc tgctgcggtg agcgacggct cttggtactc ggtgccgggc
     1561 ccagtgtcgg ctcggtcatt cgagagggtg atcgtccgca ccctgacggc cgttgccaaa
     1621 atgtctccac gacgcgggtg gattcgaggg gattcgggga cactaccggg aacggtagtg
     1681 tccccgcaac tgattgagct caagacggtt accaccagaa gtcgcgcgcc atcaaccact
     1741 tagaaacacc ggatctcagg gttcgaatcc ctgcctcacc gccagccttc gctccccttc
     1801 ggggagctac ggctggcaag ccagctgtct gtaactcatt gcatgcgaag gctgtctcgc
     1861 cgtagcggcg tggccgcgaa ggcggacaaa cggcctctac cctgtccgcg agccgccagg
     1921 ttcgttccct tcaagccttc tcagggagcc ccgagcaacg tcgaggggcc catggcaggc
     1981 cagcccaccc cgtactccgt gcggccggac tcaacagcct tagtcaagga ttggccctcg
     2041 tcgaaggttt catctgcttc acgtttgacg taatgtcata tgtgacataa cgtgaactcg
     2101 atgcctctac gatgccgccg ccggcaagag gtagggcatg aagaagggta agcgcgcgcg
     2161 cctcgaggct cacgggtggc gtgtgggaaa cgccgccgac ttcctcgaac tgagcgcgga
     2221 agagtccgca ctcgtggaaa ccaagctcgc gttgagcgag agcgtgcgtg agcggaggag
     2281 caagcagggg ctctcgcagg cggaactcgc caagcgcctg aagtcgagcc agtcacgggt
     2341 ggccaagatc gaggcggcgg atccgagcgt ttccaccgac ctggtgctgc gcgccctctt
     2401 tgccctcgga gccacccgaa acgacgtggc gtcggcgatc cgaaaaggac gaccggtggc
     2461 ggcgtgacgg ccccgaagtt gcgagcgaag cgagcgttcg ccgaaagcag accctacacc
     2521 tgtcacgtcc tgaagcgcca accgcggtgg ccacccgctc gaccccaact cggtccgggc
     2581 ggaagacggc cgggcgatcc tggcgggagg cggcgctgcg tcgccagtct cggtgcggac
     2641 gctggtgacg ccttccatca agctgatgac ggtgcttctc tggctcgcgc cgttcgcctt
     2701 tctgccgctc gcttctccgc tcctgctcct cgcgctgccg ttcgcgctta cgcgcttcct
     2761 gtccgactcg ccatttcact ggggcccggc attccattac tcggcgccac tggcacccgt
     2821 cctggccttg gccgcggcgg acggactggc acgccttcag cggcacatcc agaacgctcg
     2881 ccgaacgcga cgtggtcgcg gcgcctacgc ggccggtgtc gcttcgagtc agtttcggcc
     2941 gtgcgccaag cggctgaagc cgagccgatc tcgggcgtcg agatcggcag gagaccacgc
     3001 ggtcgtggcg tgcgtccaaa tgtctgtgtc gctgacctgg gggctgctga gcgccatcgt
     3061 cgtgaccgcc gtcctcttgc tctcggtgtc tcctctcgag gcgcagggta cgcaggcttc
     3121 ccctcctctg ccggcgtcgc tggagcggat ccggaaggag cttgcgaaac cgccggcgcg
     3181 ccgcctcaag ctcgacggga tccagacctc cgtgccggtg ttcagggcca cggtcgaacg
     3241 cgaggacgtg ccttccttca aggcactgct cgagaaggag ttccggctca acgacttcca
     3301 gcggcagtcg caggagtggt cgtcgaagtg ctgcggcctc gatctgatcg ggctcgtgaa
     3361 gggcgtggac aagtatctga agaagcggga ggcgcgcaag atccgcgagc agattgcacg
     3421 ggaactggcg cagatcgaag cggcctcgaa gaagtaagcc ggacctggca gcgggcgacg
     3481 ccccggtcgt gggcaacgcg ccccatccat cccgcccacg aatcccaccg actactccgg
     3541 ttatccccgt tgcacgacga agtacggctg gttgagcagg tcgccggaga agcccgggta
     3601 gagcgtcacg gacccgcgtc cgtgcgtcag ctggaagtaa tacatcagcg ggtacggcga
     3661 gtcggtgtac tccgcgggga cgacggcccg gtgcgacccg ctgccggcgg tcattgtcac
     3721 cgtttgaaag cgctcggcct ggttcacgtg ccggtagtag aggacggcag acgagggcgt
     3781 gacccggccg cggtgcacga tctcgagcgg cagttcctcg cccggccgga agcggcgcgg
     3841 cggctcatgg ttgcacgcgg gcggctcgcg ccgcgggcgg cccagcacgg cggcgacagc
     3901 cgcggcaacc gccgggtctg tcgagcgcga cgcaccggca agtcgctcct ccatttgagc
     3961 gatgtcggcg tcgatgaggg gcaggcggtc ggcccaggca ccgcggttcg agatcttgtc
     4021 gctcgccgaa aggtcgtcgg cgtagacacc cttcgcgcaa gcggccacgc tcgcccaggc
     4081 ggcgcgcgcc gctttgtacg tgcgaatcgc cgcctccagc gccggccgat ccccggtgcg
     4141 ctcgtgaatc gagtacagca ccccgctccg caacttcgcc gcgaagaacc ggccgagccc
     4201 gatcaggatc tcgatgtcga tgcccgtacg ccgccagtcg agcgggcgca gcggtcccgc
     4261 ccgccggaga tcctcctcgc cgcgctgtgc caggtcggcg agccactggg agacttcgac
     4321 ggacgagtac ttgccgctcg ggcggcgctg cagcagctcc tcggcgcagt cggacatgct
     4381 cgagaacaac tgcgggtcga gcgcgctcac gtgctggaac gtcttcggcg acggcgagtc
     4441 ggtgtacggg ttcggatcgg actcgcgaac catcggctgg ttccagtaca cctcgggcca
     4501 gtaggcatcg caggcggccg acggcatgtg gctgatgctc accagcggca ggatgcggct
     4561 ggtgcgcgcc aggctcgact cgagcgcgcg gccggcaggt gagcctccga aatggcgctg
     4621 ccagaccgcg ggcggcgtct ccgggttgta gaggtgccgg ccccacacgc ggtaccagta
     4681 gccgtacttc tgccagtccc actgcggatc cagctgcgga tcggcgtagc cgatgcggct
     4741 ccctccggcc ttccccgttc cccgccggcc acggcacgtc accggctcca tcaggtcggt
     4801 cccagccgag tcgcagaact ggaacgcgcg cgaataggcg gcggccgccg ccgggtctcc
     4861 ccacagcagc aggcgctgcg tccccgagaa cacgcggtgg gtcacggtgt acctgcgatc
     4921 gtcgcgcagc aggtcgccgt agccgtagcg ggtgatgctg cgcgagccct cgctcaacgt
     4981 catcagcccc tggccgcttc gcccctcgac cggcatctcg tgctcgcgga tcgccgcctg
     5041 gtggtaggga agcccctggt gctcggccca gcccttcaac gcgacgttga ccggcatgcc
     5101 ggtgtcgagc gcggcgtcga tcatcctggt gtcgagaccc ttggcgtgca ggtcgatctc
     5161 cacccgccgg ccgcagcgcg cgaccccgtc gaagatcgtc cgccagaagg cgtagttgcc
     5221 ttcgggcacc ccactttccc cgtgcgttcg cagcgcgacg ctcgagatct cggggcaggc
     5281 ccgcagcagc gcggcaagcg cgtcgcggca gtaggcggcg tgggtttccg gcgtcaggcc
     5341 ttcgatcgtg tgctgcgctc gggggctgtc atgccagacg tagccgtgca tccagattcc
     5401 cagctggaag tcgatcccgc gagccaccgt ctgccgcgcg atgaaccgca gcgtgtcgag
     5461 gttgcgatcg cgctcctcgt ctgccaggtt ggccgcgcga acgcggtacc ccgggacctc
     5521 caggaggaac ggatacgcga agaggaagta ggcatcatcg acgtggcgca ggaagtcgta
     5581 gccgaggccg aacgccaggt ggaagcggtt gaagcggtgc gtggcgagca tgtcgaggta
     5641 tgccggccac atcccgcgat cgtagaacca cggcttgtcg aggggttcgc tcgtgaactg
     5701 ccgcatcacg ctgcggacgc ggttggcggg ccgctccacg acaggcgtct cgacgtggag
     5761 cgcggcgtgc ggccgcgccg cgtgccgggc tcgatccgca agttccgtca gggcgtaagc
     5821 gagaccgcgc gcgtccggcc cgcaggcgac ggtggccggg cggcgcgaca gcttggctgc
     5881 ggcaagcacg aggctctcag gcacgtccgg cggcgtcagc ccgctgcctg ccagcgcgct
     5941 ctgcaccatt ggggaccggg cgccgcccgc cacgatgcac gccgcgtcgg aaggcacgtc
     6001 gcgcaggtcg tggcgatcga gcatcgccac gcccgccccc gacagcgcgc ttcgcagttc
     6061 cccgatggcc caccggccgg cagccgagga ggcgacaggg tcttccggat cgacaatcaa
     6121 cgcaaccgaa gcggcacgct cgccgcgaga cagcgccggc aacccgccca gcaccgcgcc
     6181 gccagccaga cccgccctga ggaagtcgcg tcttctcatc ggagccccgc ttgtcattca
     6241 gcaccgctac aggcaactcc caacatgggt tgagcaagga agtgccttct accgcggttc
     6301 gccgtccatc ccgcgaagct gctcgtccaa cgcgctgatc gcctgttcga gcatggcgcg
     6361 gtggccgggc ttgaccgcgc gcgccaggtc ggcccgggcc ttcgcccgct gcagctcgag
     6421 cgtcagccgc ctggccgccc gttcgcgctg ctcgggcgtg cgcgggccgg caacccgctt
     6481 ggtgccgcgc tcgtgctcgt cctgctgtga ttcgaccgac ttgctctccc atccccgggc
     6541 catggcgtaa tcctacagcg ggggcaccct cgcctccagc ggctgctggc agcccctcca
     6601 cctccgccct acaatagtga ggttgtactc gcaagccctt gagccttgag ccttgagcct
     6661 taagccttga gccgtctcgc cgccttgagc cctgagccct gagccctgag tcctgagcct
     6721 ttcctatgtc ttctccccgc gtccgtttcg ccccgtcccc caccggttac ctgcacgtcg
     6781 gaggcgcccg caccgcgctc ttcaactggc tctacgcgcg gcacaacggc ggcaccttcg
     6841 tgctgcggat cgaggacacc gacctcgaac ggtcgtccgc cgagatggtg accggcatcc
     6901 tcgacgggat gcggtggctg gggctcgact gggacgaggg ccccgaggtg ggaggggctc
     6961 acgggccgta cttccagtcc gagcgcctcg agcgctaccg ctcggcggcc gagtcgctcg
     7021 tcgcctcggg ccacgcctac tactgctact gcagcccgga ggaactgaag gcgaagcggg
     7081 cggccgccga ggccgcgggc ggcgacgcag ccgccgcctg gcagtacgac cgcacctgcg
     7141 cgaacctgcc cgccagcgag atcgcgcggc gcgaggctgc cgcgatgccc cgcgccatcc
     7201 gcttccggat gcccccgggc accaccgcgt acgacgacgt ggtgcacggg cgaatcgagt
     7261 tcgacaacgc ggtgatcgaa gacttcgtcg tcctgcgttc cgaccgtcat cccacctacc
     7321 acctgtcggt cgtcgtcgac gacatcgaca tgcgcatcac cgacatcgtc cgcggcgacg
     7381 atcacatctc gaacacgccg aagcaggtgc tgctctacca ggcgttcgac gccgcgctgc
     7441 cgcgcttcgc ccacgtgccg ctgatcctcg gccccgacaa gcgccggctc agcaagcgcc
     7501 acggagcgac ctcggtgatg gaatacgcgc gccaggggtt tctccccgag gcgatgttca
     7561 acttcctcgc gctgctcggg tggtcgccgg gcgacgaccg cgagttcttc acccgcggcg
     7621 agctgatcga ggcgttcacg ctcgagggca tcagctcgag caacgcagtc ttcaaccccg
     7681 agaagctcga ctggttcaac agccagcacc tggcgcggct gagcggcgac gagctgaccg
     7741 gccgcctcga gccgctgctg cgcgagcgcg gctggtggcg cgagagtctc cggggcccgg
     7801 aacggccgtg gttccaccag gtgctcgagg tgctgaagcc gcgcgcgcgg aaggtggccg
     7861 acctggtcga gcaggggcgc tatttcttcg aggcgcccac cggctacgac gccggcgccg
     7921 tgaagaagca cctgtcggat cccgcgctcg ccggaccgct gctggcgtgg cgcgaggcga
     7981 tcctcgccgt cgagccgttc gatcccccgt ccatcgagca ggccttgaga gcaaccgccg
     8041 cggcgagagg ggtgaaagcg gcagccctga ttcacgccac gcggatcgcc ctcacgggac
     8101 aggcggtgag cccaagcctc ttcgaggtgg ctgcgctggt gggacgcgag gcgagcgcca
     8161 cccggatcga ggcgctgcac cggttcctcg aggccggcaa gtagggcttt ctcggcactt
     8221 gcctccacac ttctctttgt gagttctccc ggaacgacac actggcgccc gcggggggcg
     8281 cctcaaaccg gtagagctat ctccaataac ggcagaggga taagggcgga cccgactcct
     8341 caggtggcac atcggttgca gcctcggccg acgactaaca gaacaaggcg ggcctccgct
     8401 gaggccgtta gcagaagccg aggagggcca gtcgtggcta aagaggatcg agggtttgcg
     8461 tcgatggacc gcgccaagca gcgcgagatt gccagcaagg gcgggaaggc ggcccaccag
     8521 aagggcacgg cgcacgagtg gacgagcgag gaggctcgcg aagccgggcg caagggcggc
     8581 atggcgagcc accgccgccg gcagcagagc ggcggacagg cctccgaatc gtccgaatcg
     8641 ggcagtcagc ggtagcccgc ccttatccgg aggctcactt ggggtcgggc cgtgaccggt
     8701 ccgaccctcg aggtcacacg ccagccacca aagcaggtca actggtcaat tcactcgcgg
     8761 cgcagggccg tgaccgggtc gacccgcgac gcgcgccacg cgggcaggaa ggccgccagc
     8821 atcgcaataa ggccgagcag cgcggcagcc gacgcgaagg tgacagggtc gcgaggggtc
     8881 acgccgtaga gcagcctgcc cagcagtcca ctcagggcca gcccgatgcc cgcccccgcc
     8941 acgagaccgg cggcgaccag ggcggcgctc tcccgcagga attggcgaac cagtccggcc
     9001 gggggagcgc ccagcgccag ccggacgccg atctccggga tccgctgcgt cgcgctgtac
     9061 gccagaacgc cgtagacgcc caccgacgcg atcagcagcg ccacgccggc gaaggccgcc
     9121 agcagcatca tcacgaagcg ccgctgggcg atcgtctcgt tcaccacctc gtccatggtg
     9181 ttcacctgcg acacgggcag ttccgggtcg aggcggcgca gctggccgag cgcctgcaac
     9241 gccatcgcgt cgggagcgct cgccgtccgg atcaccacgc ccatcagcgg aaaggccatc
     9301 tgccggttcg tcaggtagat catcggccga acgtcgcggt cgagggcctc gtgcctgacg
     9361 tcgcccacga ccccgacgat ctgcgcctcg acagagccat tctcgccaag ccgcacgcgg
     9421 agccaccggc cgagtgggtt ctcgtcaggg aagatctgcc ggaccagcgc ctcgttgacg
     9481 atggctgcgc cgcgccccgc ttcgccgtcc cgagcctcga agagccttcc ccttcgcagc
     9541 ggaatcctca tcgtctcgaa gtagcggccg gcgatgatgc ggatgtcggc cgacgggagg
     9601 ttcgcctcgc cggggaacgg ccggccctcg atcgagaagc cggtggcgat ggggacgccg
     9661 cggaacggga agccgctcga gacgccggcg gagcgtacgc cgggcacacg tgacagctcc
     9721 tcgatcaggc ggtcgaacaa ggcggcccgg ctcgcgggct cctcgtaccg cgattgctga
     9781 agcagcaccc gcatcgtgag cacgtggtcc gggcggaagc cggggtccac gtcgagcagg
     9841 ctgccgatgc tgcggatcat caaacccgcc gccacgagaa gcgtgaaggc gagcgcgagc
     9901 tgcgcggcgg cgaggacgcg ccggagatgg cggtgcgagc ggctctcctt gcccgcccgg
     9961 gcgccctcgc ggagggctcc ttgtatgcgc atccgcgaga ggctcagcgc ggggacgagc
    10021 gcgaacacca gcaccgtgcc cagcgacagc gccgacgcga aggccagcac gccgccatcc
    10081 agcgtgatct cgtcggcggg tcgcggcagc gacagcaggt gcacggcggc cgcgagcagg
    10141 tagtcgagcg accaccacgc gagcagtacg gcgaggcttc cgccaatcgc cgccagcacg
    10201 acgccctcgg tgagcagctg ctggacgatg cgccaccggc ctgcgccgag agccgtccgg
    10261 agtcccagct cgcgctcgcg ccccaggccc cggttcaacg acaggttggc gacgttgacg
    10321 caggcgagca ggagcacgaa gccgacggcg ccgaacagca cgagcagcac cgggcgaatc
    10381 tccccgagca gctgctcgtt gagcgatacc acccgcacgc cccacccctc gtcgaagtcc
    10441 ggcagctcgg cgcgcagcga cgcggcgatc gcctcgaact cggatcgggc gtcctcgagc
    10501 gacaccccgt cgcgcagccg tccgaccacc gtcaggtacc ggccgcgcgg cgtgcgcgcc
    10561 tcgactggga aggcgatcgg cgtccagaac tcgctggccg gagagaggat tgagaacccc
    10621 ggcggcatca ccccgaccac cgtcaccgcc tcgccgccga gcgtgatgcc gcgtccgacg
    10681 atggccgggt cggcgttgaa gcggcgccgc cagagccgat cggaaaggac gaccacggca
    10741 tcgccgccgg ggcggtcctc gctttcgaga aacgtgcggc ccagcgcggg catcgcaccg
    10801 agcatcggga agaggctggc attgacggcc tggccttgca gctcttccgg ttcaccggcg
    10861 gcggtcagcg tcgccgtgct cgtggcgatc gccgccatct gcgagaacgt ccggcttcgc
    10921 tcctgccagc ggaggaagtt ggcgggcgac accacgttgg ccgggttgtc gctgtcgagg
    10981 cgccgctccc agatgacggc caggcggtcg gattgccggt agggcagggg ccgcagcagg
    11041 acggcgttga ccaggctgaa gaccgccgtg ttggcgccga tgccgagcgc cagcgtgcag
    11101 gcggccaccg ccgtgaagcc ccggggcacc tgcttcttgc ggaagtcgag acgggcatgg
    11161 aaagcgtttc tccggcacaa tggggcatgg ccgattcagc ccccgggttt gccatcgccc
    11221 cgcccatcga gccaatgctc gcgcgcctgg ccgacaccct gccggcgggc gacttcatct
    11281 acgagcccaa gtgggacggc ttccgcgcga tcgtcttccg cgggggcgcc gaggtcttca
    11341 tccagagccg cgacctgcgg ccgctcgacc ggtacttccc ggaactgcac gcggagttcc
    11401 tggcggcgct gcccgaaggg tgtgttctcg acggggagat cgtgatcgcc gccccctcgg
    11461 gcctcgactt cgacgcgctg cagatgcggc tgcacccggc cgcgtcgcgc gtcgcgaagc
    11521 tggccggcga gacgcccgcc tcgttcgtcg cgttcgattt gctcgccgag tccggccgcg
    11581 acctgcgtgc gctgccgcag cgcgagcgcc gcgaccggct cgagcggctg ctcggcgccg
    11641 cgactccccc gatccacctc acgccggcga cgctcgatct cgggacggca accgagtggc
    11701 tggcacggtt cgagggggcc gggctcgacg gcgtggtggc caagctgtcc gagcttcagt
    11761 accagcccgg caagcgcgcc atggtgaagg tgaagcacgc gcgcaccgcc gactgcgtgg
    11821 tgggcggctt tcgctggcac aagagcgggc acgaccgggt cggctcgctg ctgctcggct
    11881 tgtacgactc cgggggaacg ctgcaccacg tcggcgtgac gtcggccttc acgatggcca
    11941 cgcggaaggc gctggccctc gagctggcgc cgctgcgcga ggacgcgctg aaggatcacc
    12001 cgtggcgcga gtgggcggag ggcggggccg acgcggcggc gcgcatgccc ggcgcgcaga
    12061 gccggtggag cgccggcaag gacctttcct gggagccgct gcggatcgaa cgcgtctgcg
    12121 aggtgaagta cgaccacctg cagggcgacc gcttccgtca cgcgacgcag ttcctgcggt
    12181 ggcggcccga caagcgcccc cgcgactgcc gctacgacca gctggaggtc acgccgccct
    12241 acgagctgga aagggtgttc ggggcagcct agctgagcct cgagctgaac ctggctgccc
    12301 cagccgggat tcaggaccgc aaggcgccca cctcgatcgg cggcgtgcgc agggtgccgg
    12361 tgtcgccgtc gtggcggacg aacatcagca cgaccgacac ccgcgcgccg atctcgacgt
    12421 agggaccatt cccgatcgcc acctccaggt ccaccgtccc tcccggggcg atcgttccat
    12481 cccacacgag cggctggccg gcgatgcgcg ggacggcggt cacagggtgg ttcccatcgc
    12541 gctcgtcgta cacgacggcg gcggcgttga accggtggtc gtcctgcccg gcgttgtgca
    12601 cccggatgtt gaccaggccc ccgagcgtcc cgccgccgtc ggggcccgga tcgagcacgc
    12661 gcggcgcgaa gtcgcggttc agggacgcgc cggtgatggt gaagctgatg cttccgatac
    12721 ggacctcggt ccgcggcgag gaggggacgt tcaacgtcgg cggcgcgaag cagccttcag
    12781 cggcgagaag ggcggccggc aggaggagta cgaacaggtt gcggcgcatg acgatcgaat
    12841 agatgaaatg cagtagtgac ctcacgatcg agctccacga gatgaacggc ggcgcgaggg
    12901 ctgcacccgg gcaggctcgt ccggctgctt gcggtagtga gggggccacg gcgcgtcgcc
    12961 ctggccgtcg cgctgctgtc gcgccgacag ttcgagcagc gcgtccagcg agcacgggtg
    13021 gtcgtcgatg cctgccaggc ggtcgcctac cgccgcgaac cgcgacggca tcgtcttcaa
    13081 ggtgaagtcg gccggatcgc agtcgccgac ctcgtcccac gacagcggcg cggagactcg
    13141 tgcgtcaggc ttgggccgca ccgagtacgc ccccgcgacc gtgcgatcct tcgcgttctg
    13201 gttgtagtcg agaaagacgc cgtggcgctc ctccttccac cacttgctgg tggccagcgc
    13261 cggggcccgg cgctcgacct cgcgcgcgaa ggcgagcgcg gcctggcgca cctggtcgaa
    13321 ggtccaccgc ggcgcaatcc ggacggccac gtgaatgccg cgggagccgg aggtcttcgg
    13381 ccaccccgtc aatccgaagt cgccgagggt cgcccgtacg atgccagcga cctcgcgcac
    13441 ctgcggccag cggacgccgg gcaccgggtc gaggtccacg cgcagctcgt cggggtgatc
    13501 gacgtcctcg gcgcgcacgg gatgcgggtg cagctcgagg cacgcgaggt ttgccatcca
    13561 cgcgagcgtg gccgccccgc gcggcaccac ctcttcggcg cttcgcccgg acgggaagcg
    13621 cagcgtgacc acctcaaccc acgatggccg cgatgcgggc gcccgcttct ggtagaagaa
    13681 ctcgccgtcc acgccgttcg gataacggac cagcacgttg ggccgccccc ccgatccccg
    13741 cagggctccc tcggccaccg cgaggtaata gcggacgagg tcgagcttgg tgtgcccggg
    13801 ctcggggaac agcaccttgc cggggttggt gacggccacc tcgacgccgt ccaccttcac
    13861 tgtctcctgg ggggccatgc agctattatg ggaaaaagcg gacaagatga cacggagggt
    13921 ccccatgagt ggtcgcgagg tcgtcatcgt cggagccgcc cggacgccaa tcggctcgtt
    13981 ccttggttcg ctggcgtcgg ttcccgcgcc gcggctcggg gcggtcgcca tccgctgtgc
    14041 gctgcagcgc gcgggagtcg atcccgcggc tgtggacgag gtcgtcatgg gcaacgtgct
    14101 gcaggccggc gtggggcagg cgccagcccg gcaggcggcg atcttcgccg gcctcccgcc
    14161 cacgacgcct tgctggacca tgaacaaggt gtgcgggtcg gggctgaagg cagtgatcag
    14221 cggggcgcag gcgatcgcgc tcggcgacgc cgaggtggtg gtcgccggcg ggatggagtc
    14281 gatgtcgaac gctccgcact acctccgcgt gcgcgccggc caacgcctcg ggcacgcgtc
    14341 gctcgtggac ggtctcgtgt tcgacggcct caccgacgcc tacgacagcc gccacatggg
    14401 ggagtgggcc gaggtgacgg ctgccgcccg cgcgatcacc cggcagatgc aggacgagtt
    14461 cgcggccgag tcgacgcggc gcgccgtcgc ggcacagaag caggggctgt tcgccgccga
    14521 gatcgcctcg gtggagatcg aggggcgcaa gggcgagaag accgtcgttt ccgaggacga
    14581 agggcccaag accgcgcggc ccgacaagat cgcgtcgctg aagccggtct tcaagaagga
    14641 cgggaccatt acggccgcca acgcctcgag catcaacgac ggcgcggcgg cggtggtgct
    14701 gatgagcgcc gagcgggcgg cgggcgaggg ccggcccgtg ctcgcacgga tcacggcctg
    14761 gggcggcgcg gcgcgaacgc ccggcgagta cacgattgcg cccaccgacg ccatccgcgc
    14821 cacgctgtcg agggcggggc tcgggatcga cgacatcgac ctctgggaga tcaacgaggc
    14881 gttcgcggtg gtgtcactgg ccaacaacat gctgctcggc ctcgaccccg ccaaggtcaa
    14941 catccgcggc ggcgccgtcg tgctcggtca cccgatcggc gccagcggcg cccgcatcct
    15001 ggtgacgctg ctccacgcgc tgagagacga gcggaagaag cgcggcgtcg cctcgctgtg
    15061 catcggcggc ggcgaggcgg tggccctcgt cgtggagcgc ccgtcggcgg gatgacgtgc
    15121 agacgctgct gatcgcctgc cgcacgctcg agagcgagat tgcgctggcc gggcgcgaag
    15181 cggggatcgc ccgcccgacg cgctgggtgg agccggggct cgacgcccgg cccgacagac
    15241 tgcgcgatct cctgcgcgac gagctgcacc gggtgtcgtg cgagaacgcc agccgcgccg
    15301 cctccaaggg ggcttccacc gggcgcgtgc tgctcgcctt cgggtactgc ggccacgcgc
    15361 tcctcggcct gcacgccgcc agcttcgagc tggtcttccc ccgggcggac gactgcatca
    15421 gcctgctgct cggatcggcc gagcggcgcg aggcggttgc cggcgagacc cccacctact
    15481 tcctcaccga gggctggctc gagcacgaga cgaacgtctg gtccgactac cagcgggcgg
    15541 tcgcgacgat gggggccgag cgggcgaagc aggtgatggc gatgctgctc gccccctacg
    15601 agcgcctcgg cgtcatcgac accggcgcct gcccgctggc gcgcgtgacc gaacgcaccc
    15661 ggccaatcgc cgagaccttc ggcctgcgtc acgaggtgat tccaggctcg acacgctacc
    15721 tgcggcagct gctcgccggg ccctgggggg acgagttcgt tacaataccc cccggcggca
    15781 cggtgaccct cgaccacttg ctgcggcagg accgcacgcc atgattcacc gaatcctctg
    15841 cccgctggac ctttccgact tctccaagcg cgccttcgag cacggcctcg tgctggcgcg
    15901 ctggtacggc gcgaaggtcg tggcgctgca cgtcttcgcc ggcttcatgc cgcccggcga
    15961 cgagagcacc tacccggcgt ggctccgccg cgtccccgag gcgcgcaagg agatcgatcg
    16021 cgagctgcac gagcaggtgt cgtcgccgct ggccgagggg atggacgtcc cgctggtcct
    16081 gcgggagggc gacccggttg cggggatcct ggccgaggcc aaggccatgg cggccgacct
    16141 ggtggtgatg agcacgcacg ggcgcagcgg gttcgatcgg ctgacgctcg gctcggtggc
    16201 gtacaaggtg ctccgcaagt cgccgtgccc cgtcctgatc gttcccgcgc agcaggccag
    16261 gcagggcgag ttccagggct accgccggat catctgcgcg acagacttct cgcccgactc
    16321 gggtcacgcg ctcgacttcg cactctcgat tgccgagcgt gccggctcgc gcctcaccat
    16381 cgctcacgtc gtcgaggtgg ccgagaaccc cgaggtgctg gagggggatt cgccgcttgc
    16441 ggtggtgaga cgggagcgaa tcgagaaggc gcacgcacgg cttcgcgagc tggtggccgc
    16501 gcgcgagggg caggagccgg cagtcgacga gacggtgagg atcgggaggc cgtacgtcga
    16561 gctgctggcg ctggcgaagg caaccgaagc cgacctgctg gtgctcggcg tgcggggacg
    16621 cggcgcgatc gatctcacgc tgttcggctc gacgaccgcc caggtggtcc gccgtgcag
//
LOCUS       DAOPQX010000041        16519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4180_length_16519_cov_4.628462, whole genome shotgun sequence.
ACCESSION   DAOPQX010000041 DAOPQX010000000
VERSION     DAOPQX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16519
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4180_length_16519_cov_4.628462"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..889)
                     /locus_tag="PKK95_03800"
     CDS             complement(125..889)
                     /locus_tag="PKK95_03800"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOC17365.1"
                     /translation="MLSRGRSLRRLGLVFLAALLLAVTLHAQRRRFWFDQVPSVTAVP
                     YDGRFTIVRLWYPHYGGWSYDYPDMEQNFTLILNDLTYVHPRPDRSSILRMDDPELMK
                     FPIAYLSEPGYWYPTDTEAQGLRTYLAKGGFLIVDDFHFEREWQVFEAAMRKVLPNAR
                     IDRLPENHPVFQSFFSIESLEVPYPGRLGESGLMGEFYGIHQDNDPSKRLMVVINYNM
                     DIGDYMEHSGRGFYAVDPTNEAFKFGVNYVIYGLTH"
     gene            complement(978..2654)
                     /locus_tag="PKK95_03805"
     CDS             complement(978..2654)
                     /locus_tag="PKK95_03805"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOC17366.1"
                     /translation="MSLLVETAVRVSLVVLAALGAVSVLSRRSAALRHWILTAGIACA
                     AAMPLLQLALPSWRIAPPALGIEEPARQADVSARVVVRGLAQPAATVQAPTPRASTGS
                     ARPFLPIVLLSAWLAGALASLWALAAGLLRLSRAAAAARKVTSGPLFSIAADTRRAIG
                     LRRDVTILQSDSAPLVTWGVVRPTVLLPADAQEWPEDRLRVVLRHELAHVVRGDWAAQ
                     MLAEALRAVLWFNPLLWIASRRLRQEGERACDDEVIGSGVGPADYASHLLDLARRAGL
                     GSVRPALAMARPTSLEGRVRAMLSKNVSRRPVKTRTRVGVLAAFAALTMPLAAAQGRF
                     WSFSGTVVDQTDRAITEAVLVLSNEAARAKFEVRTDRAGRFEFQALPSGDYVLAVQKP
                     GFKTLKQALTIGSDASRTIKLQVGSVTESIRVVSGPTTPVADSEQIAGRQRARDEARK
                     RTSERCGGGEAAGEGGGELLPPLKLVDVRPAYPEGLKAAGVAGVVTLDAVIGADGGVH
                     DVTVVSSAHRDLERLAIDAVRQWEFSPTYLNCVPIDVEMRVTISFAEGGK"
     gene            complement(2651..3043)
                     /locus_tag="PKK95_03810"
     CDS             complement(2651..3043)
                     /locus_tag="PKK95_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOC17367.1"
                     /translation="MRQPVHAALSRRERQIIDILYRLGRATAAEVRRELPGDPAYSTV
                     RTQLRVLESKGHVRHEEEGLRYVYTPALPRTVASKSALRHLVDTFFGGSTERVVAALL
                     GPDGGRVSADELERIAQVVARARKEGER"
     gene            complement(3152..5224)
                     /locus_tag="PKK95_03815"
     CDS             complement(3152..5224)
                     /locus_tag="PKK95_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC17368.1"
                     /translation="MSPGVGRRIALVHVLALFALAVSYPLLDMLGRSPEFFVAQRLEG
                     RDLWVFLALVSVAAPLLFVLLAWVAGRLGGWAASCTVGTLRTLLAAAVVLQAIRHLPA
                     PGWGLALASMAAGLAVEAGCRRSSSVRLFVALLVPAFLLAPLLFVLSSPSSRLLRPVE
                     SVFFGDAKPAVNVPVVLVVFDQLPLVSLMDAGGGLDRAAYPNFAALADTATWYRLATS
                     VADRTEMAVPAILTGRYPRRGETPTASSHPDNLFTGLAPAYALHVQEPMTRLCPPALC
                     PDPTPLTRRLAAAGLDASVAYLHRLLPADLAEGLPDVRHDWRGFWHQDAQDRFKASNS
                     GDRHPYEWIDTIEPDAERPTLYFAHVLLPHEPFVYLPSGQTATTLRHLPGLYGDEQWV
                     TDDWQVAVNYQRHLLQVGAADRLLGLLLDRLRSRGEFDRALVIVTSDHGASFQPGGHY
                     KKPDRRNFADIMNVPLLVKLPGQREGRVSDRPAETIDIVPTVMDVLRIPRSGQRDGRS
                     LLADGAPKTSVRMYFNGATRRHRTEPQRIAAASLESARRKIEIFGPGPAWSPRRNYTA
                     ELVGRNVADFEVSPQTALDVRIDRKSMYRSVDPEGAFVPALITGTIRALQGTLDHQVL
                     AVAVNGTIRATSRVVTSGSRDEGRWSAMVRPECLRAGANDVAVYEVRAEGGRFTFRSL
                     VPPAAGGA"
     gene            complement(5221..5622)
                     /locus_tag="PKK95_03820"
     CDS             complement(5221..5622)
                     /locus_tag="PKK95_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17369.1"
                     /translation="MSATDELGYPNEPLCREPWESYYILRRGVLPCCYGSTPIAPMAE
                     WRTAWNSPALQEIRSHLVRGELSPYCLASLGCPVVQRFLDERRRSPLAAIDPRARSPL
                     LRGLNRLLGGVPARLYRLLSSARGGRGRASR"
     gene            complement(5636..7726)
                     /locus_tag="PKK95_03825"
     CDS             complement(5636..7726)
                     /locus_tag="PKK95_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC17370.1"
                     /translation="MQTTFNITYGDGWYPVEHDGARPFRWSGPLARVRIAAESPCRSV
                     WLRLACGVWGAAPSVPSLSLAVNGRTTGSAQVAEQLGYLAFPLGGGRDFDVELRLDST
                     FSVPGDPRMLGVMVKGLDAFDEDALDSPLELDGWYERETHEYFPFSWMGREASVLVPA
                     LVRPGTRFLTLPIAAETDAGQQVLAIWRDERLLAELPLRRGWHLYDVDVLDGAAQSIA
                     GEPASARLTLRLSALCPPEARPGDPRALGVRVGPIEAHGDRRRHAHVNSFHHTVRVED
                     VDRAARQSAGEVCCGVPAAGEGWNGEERDERGVFRWTRREAALLLRDSLRCGRRFCSL
                     PLFSAFRNLAQQVTVSAAGRELARFDLAKGWHTYSVALPDPGGDLEIILRVNVLTPAS
                     SHAGDPRELGIRVGALTLHDDERRHEREVFLAANAVRNQREMQRGATVVESFPQTLGI
                     DLYGRCNIKPACVYCLWDPMKALEGEAVDETVDDRTLEGYGGLFEGARLLVNCSFGEP
                     LLHPRLDRVLDLAASSGKVMELSTNGQAFTPRTVGLLAGRRVHLYVSLDAASGATYAR
                     LRNERWDEVLAGLLFLRDAREAGGGWPRLNMVFMPMPANLDDLESYFRLCRLVEADTL
                     VLRPLLHLEHPGIVRERGGYRFDYERELMTPEALEPVFAACERYAARYEVSVVSQFDF
                     GLDEASRFRPTATL"
     gene            7949..8587
                     /locus_tag="PKK95_03830"
     CDS             7949..8587
                     /locus_tag="PKK95_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17371.1"
                     /translation="MVRLAVVVTLVLAPAASGALAQSPVEGQGSAAQAQGAAQPLESE
                     RPEAIIDRLPISLEAIRSGIERAPEAPSALRIGQLPTFRVTIYGQRRQLLPDFQETLR
                     QPWQAPIPGGIHNKEVLDMITPPEARSFGAFTNGDLAEVAGTALVSGLAARALYNGYT
                     AVKGALRSWQEERIRREVEAELEAFKRANGIVDPSPRSQTPSKPPGPEEEKK"
     gene            complement(8608..9003)
                     /locus_tag="PKK95_03835"
     CDS             complement(8608..9003)
                     /locus_tag="PKK95_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC17372.1"
                     /translation="MVSADRQIRVLLVDDSAEIREMYALYLAGAGCEVEQASQGFEAF
                     DKAIKGRHDAIVMDLRMPKLDGWEAVRLLKNRAQTRGTPIIALTGDSDIEHLKLARNA
                     GCDTVLLKPCAPDRLHAAIVKLVGPTLAA"
     gene            complement(9367..9750)
                     /locus_tag="PKK95_03840"
     CDS             complement(9367..9750)
                     /locus_tag="PKK95_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC17373.1"
                     /translation="MDATDSSPLVLIVDDDPDGRNLYSYALQAAGFRVEEAADGFEAV
                     DKGYKLKPQVILMDLLMPRLDGWEVVSWLKKNPTTREIPIVAVTGAQEDIQQMAVAAG
                     CRAVLTKPCPSGRVVDEVRQVLAAR"
     gene            9841..10116
                     /locus_tag="PKK95_03845"
     CDS             9841..10116
                     /locus_tag="PKK95_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOC17374.1"
                     /translation="MIVARKLTLSGRVQRVGFRWFAVEAAEREGVAGWVANTRDGRVE
                     IFVEGDAEAVERFERAVRRGPIAARVDEVETEVRPPSGRVAGFEVRR"
     gene            10158..10673
                     /locus_tag="PKK95_03850"
     CDS             10158..10673
                     /locus_tag="PKK95_03850"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOC17375.1"
                     /translation="MDTLKARIRSVPDFPKAGILFYDITTLLGDPAGFRAALDALALP
                     YRDQGISVVVGIESRGFILGGALADRLGAGFCPVRKPGKLPARTISESFQLEYGTDRL
                     EMHEDGIKPGQRVLIVDDVLATGGTAAATARLVRRLGGELHGLAFLIELLTLDGRSRL
                     AGERVNSVLKY"
     gene            10711..10788
                     /locus_tag="PKK95_03855"
     tRNA            10711..10788
                     /locus_tag="PKK95_03855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10746..10748,aa:Arg,seq:cct)
     gene            10906..11634
                     /locus_tag="PKK95_03860"
     CDS             10906..11634
                     /locus_tag="PKK95_03860"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC17376.1"
                     /translation="MKRIERRRLKENEVALSVARARETFDIYRKPIIAGLVTLALIVV
                     AVVGVTAWRKSADSNARTLLADAMAVAGAPVVPATPGPNTPAPSPGSFGSEKAKLEAA
                     LQKFMAAAAAHPSSEPGIAARYHAAAILATLGRHKEAEQRYREVIEKPGAGLYGPMAR
                     LGLANTQAAAGQRDQAIAAYKELSARKDSDLPVDAILMQLARTYLQDGKTAEAQQTLR
                     RIGDEFPQSPYASLAAKELGQIKN"
     gene            complement(11701..12588)
                     /gene="speB"
                     /locus_tag="PKK95_03865"
     CDS             complement(11701..12588)
                     /gene="speB"
                     /locus_tag="PKK95_03865"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOC17377.1"
                     /translation="MKPPVEYEHPSPLSFGGYEPARSFDDARAIVLPVPFERTTSYVT
                     GTRNGPREILLASGQVELWDEETGTDAWSRGIVTLPEMELPYNDMRVALDEIRRVVSE
                     LIPHEKFLVVLGGEHSISVPVVEAYAAATPNLSVLQIDAHADMRDSYQGSRYSHACAM
                     RRIVELVPCTQVGIRSLSREESRVIPSLRTRVFWDHAMRQDPDWIEKVVEGLSEAVYI
                     TIDADGLDPAIMPAVGTPEPGGLSWPETTALLKAVLSRRRVVACDLVELNPLPGMVGP
                     NFLCAKLIYKILSYRFRQA"
     gene            complement(12644..15586)
                     /locus_tag="PKK95_03870"
     CDS             complement(12644..15586)
                     /locus_tag="PKK95_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOC17378.1"
                     /translation="MRRLALLALLLSLAASEAAAASRYLPHLRFRVLVTPHFRIYYHQ
                     GGEQLAREVAAIAESVQRELPARLGLPAPAVTHVVLADQDDASNGSAFPVPYNTIALT
                     AAWPAQSELIGFTREWLRLVFVHEYAHILQTNQSRGWASVMRGIFGRSPLAFPNLALP
                     LWQIEGFATWVESRETGEGRVRAGDAATIVRQRVREAGPEPIDRYNGGLVDWPGGNGS
                     YLVGGFFHQYLVSRFGERKLGDLVERQAGRVHYFGAGAFEAVLGVPLPDLWRDFMNSV
                     APASLPASGAPASGPAVPARLTRHGFLVGSPRFAPDGSALFYTLQTPHRFPAIMRLDR
                     GHGADEGPAGIPREIATQYGAEGLSVGDRVVIFDQLELETNVALRADLYAVSPRGGAV
                     AALTESARLMQPSLSPDGASLVAIRIAGGRRELAWFDVRRKGGEGIRLEPRPGPDGGG
                     QAASPRWSPDGRLVAFERRVPGGPSEIAVADPATGSIRVLVSSALYRLVTPVWAPGGE
                     SVVFASDHPPGAAPGALLARSFQLYTVPVEGGLVRRLTAVPGGATSPAISPDGRRVVF
                     VGYTEEGADLFALPFDPASAADAGWTGPAPVASAAAAPLAQVEDRGYSPFDTLRPRAW
                     TPVADTGDTLRVGLALGGIDVLGRHAWGALLRWRVGSEGDAVNGLHRGRPDVDLGYTY
                     DRWRVPLWISASDETSFLAVRNQSGGRLPDAELRERSVSAGLMLPFARIRRLQLWQAA
                     FKYERSTLQATRADAPRTFLRHAIEAGWRLDTARRYGYSISQEEGVSLGIGTEHVRRA
                     FGADGDAQAWTAQARVFRRLGGRHAVLAARAGGAFSSGDRNVRRVFFLGGAAPARSVV
                     DVRSEAVEMLRGLENEAYVGTRVVSGSLEYRFPLARVERGWRTAPLFLRAVHGAVFAD
                     AGHAWGAGLPFAWPDFKTSVGAELSLDTVVGFGLPLTFTGGIAWARDGATGRHLDPRP
                     YVRIGRSF"
     gene            complement(15583..>16519)
                     /locus_tag="PKK95_03875"
     CDS             complement(15583..>16519)
                     /locus_tag="PKK95_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17379.1"
                     /translation="LAEVATVNNYTKPHVHDADEIVITEGRHPVVERRSRDPFVPNDT
                     LLDGSGHQLIVLTGPNMGGKSTYLRQTALVCLLAQVGSFVPAREAKLALVDRIFARVG
                     ASDNIARGQSTFMVEMQETANILHSATSRSLVILDEIGRGTATFDGLSLAWAVAEHLA
                     TNSRARPKTIFATHYHELTDLADGIPGVVNCHVDAREWHDEILFLRKVVPGRADRSYG
                     IQVARLAGLPPKVVERAREILSALEHDELARGGRPSLSRTPTEPQQQLGLFQALPSPR
                     AERLVERLLAVDPDHLTPLEALTLLAELRRDAQES"
BASE COUNT         2652 a         5792 c         5811 g         2264 t
ORIGIN      
        1 gagcactcgg ttcgggaccg aggggtcgga ggttcaaatc ctctcgcccc gaccactcaa
       61 tcaagacccg caagaagcct ttcattggcc gtgatggccg gtgaagggct tttttgttgc
      121 cgactcagtg cgtcagcccg tagatgacgt agttcacgcc gaacttgaac gcttcgttgg
      181 tcggatccac cgcgtagaag ccgcggccgg agtgctccat gtagtcgcca atgtccatgt
      241 tgtagttgat gacgaccatc aggcgcttcg acgggtcgtt gtcctggtgg atcccgtaga
      301 actcgcccat caggccgctc tcaccaagcc gcccgggata ggggacctcg agcgactcga
      361 tcgagaagaa cgactggaag accgggtggt tctctggaag acggtcgatc cgggcgttcg
      421 gcagcacctt ccgcatcgcg gcctcgaaga cctgccactc gcgctcgaag tggaagtcgt
      481 ccacgatcag gaacccgccc ttggccaggt acgtgcgcag cccctgcgcc tcggtgtcgg
      541 tcggatacca gtaaccgggc tccgacaggt aggcgatggg gaacttcatc agctcagggt
      601 catccatccg caggatgctg ctgcggtccg gtcgcgggtg gacgtacgtc agatcgttca
      661 ggatgagcgt gaagttctgc tccatgtccg ggtagtcgta cgaccaccct ccgtagtgcg
      721 ggtaccacag gcgcacgatc gtgaaccgcc cgtcgtacgg caccgcggtc acagatggaa
      781 cctgatcgaa ccagaagcgc cgccgctgcg cgtgaagcgt cacggcgagc agtagcgccg
      841 cgaggaagac gagcccgagg cggcggagag aacgtcctcg agaaagcatg ggggcactga
      901 gacgtactct agaggggaag acggcagggt gaaaggaaaa aggggagggc gcctgcatgg
      961 ccctccccga agctccgcta tttgccccct tccgcaaagc tgatggtcac ccgcatctcg
     1021 acgtcgatgg ggacgcagtt caggtaggtc gggctgaact cccactgccg caccgcgtcg
     1081 atcgccaacc gctcgaggtc gcggtgggcc gacgagacga cggtcacgtc gtgcacgccg
     1141 ccgtccgcgc cgatgacggc gtccagcgtc accacgccgg caaccccggc agccttcagg
     1201 ccctccgggt aggccggccg gacgtccacc agcttgaggg gcggcaggag ctcgccgccg
     1261 ccctcgccgg ccgcctcccc gccgccgcat cgctcggagg tgcgcttgcg cgcttcgtcc
     1321 cgagcccgct ggcgtccggc gatctgttcg gagtcggcga ccggagtcgt ggggccggag
     1381 acgaccctga tcgactcggt gacgcttccg acctgcagct tgatcgtccg gctcgcatcg
     1441 ctgccgatcg tcagagcctg cttgagcgtc ttgaagcccg gcttctgcac cgccagcacg
     1501 tagtcgccgg agggaagcgc ctggaattcg aagcgaccgg cccggtccgt gcgcacctcg
     1561 aacttcgctc gcgccgcctc gttcgacagc acgagcacgg cctcggtgat ggcgcgatcc
     1621 gtctggtcga ccacggttcc agagaaggac cagaaacggc cctgcgcggc ggcgagcggc
     1681 atggtgagcg cggcgaaggc agccaggacg ccaacccgcg tcctcgtctt caccgggcgg
     1741 cgactgacgt tcttgctcag catggcacgc actctccctt caaggctcgt tggccgagcc
     1801 atcgccaggg cggggcgtac cgaccccagc ccagcgcgcc tcgccagatc gagcagatgc
     1861 gacgcgtagt cggccggccc gacgcccgac ccgatcacct cgtcgtcaca ggcacgttct
     1921 ccttcctggc gcagccgccg gctcgcaatc cagagcagcg ggttgaacca gagcacggcg
     1981 cgaagagcct ctgcgagcat ctgggccgcc caatcgccgc ggacgacgtg cgcgagctcg
     2041 tgccgcagga ccactcggag acggtcctcc ggccactcct gcgcgtcggc cggcaggagc
     2101 accgtgggcc gcacgacgcc ccaggtcacc aggggcgccg aatcgctttg tagaatcgtg
     2161 acgtcacggc gcagaccgat ggcgcgccgg gtgtcggctg cgatcgagaa gagcggaccc
     2221 gacgtgactt tccgcgcggc cgcggcagcc cgcgacagcc gcaagagtcc cgcggccagc
     2281 gcccagaggc tcgcgagcgc gccggcgagc caggccgaga gcaggacgat cggcaggaaa
     2341 gggcgggcgc ttccggtgct cgcgcgaggc gtcggcgcct gcaccgtcgc cgccggctgg
     2401 gcgagccccc ggacgacgac ccgcgcgctc acgtcggcct ggcgcgcggg ctcctcgatc
     2461 cccaacgccg ggggagcgat ccgccacgac ggcagcgcca gctggagcag cggcatcgcg
     2521 gccgcgcagg cgatgccggc cgtgaggatc cagtgccgga gggctgccga gcgccgcgac
     2581 aggaccgaaa cggcgccaag agcggcgaga acgacgagcg aaacgcgtac ggccgtctcg
     2641 acaagcaggc tcatcgctcg ccttcctttc gggcccgcgc cacgacctgg gcgatccgct
     2701 ccagctcgtc ggccgaaacc cttccgccgt ccggacccag cagggccgca accactcgct
     2761 cggtcgagcc gccgaagaag gtgtccacca ggtggcggag cgccgacttg ctggcgacgg
     2821 ttcggggcag ggcgggggtg tacacgtacc gcaacccttc ctcctcgtgc cggacgtggc
     2881 ctttcgactc gaggacgcgg agctgggtgc ggaccgtcga gtaggcagga tcgccgggca
     2941 gttcccgccg cacctcggcc gccgtcgccc ggcccaggcg gtagaggatg tcgatgatct
     3001 ggcgctcgcg gcgggagagc gcagcgtgaa ccggctgccg catgatgcga tgaatataac
     3061 aggctgctat aaaaatagca agaatggtcg cccgcgctcg tagaactcgt ggcaggggca
     3121 gggtcactgg cagagagaaa agggcggacg ctcaggctcc tccggcggcg ggcggcacca
     3181 ggctgcggaa cgtgaagcgg cctccctcgg cccgcacctc gtacaccgcg acgtcgttgg
     3241 cgcccgcgcg caggcactcg ggccgcacca tcgccgacca cctcccctcg tcgcgagagc
     3301 ccgaggtcac cacgcgggag gtggcgcgga tggtgccgtt gacggccacg gccagcacct
     3361 ggtggtcgag ggttccctgc agcgcccgga tggtaccggt gatcagcgcg ggcacgaagg
     3421 cgccctccgg gtcgaccgat ctgtacatac tcttccgatc aatccgaaca tccagggcgg
     3481 tctgcgggga aacctcgaag tccgcaacgt tccgcccgac cagctcggcg gtgtagttcc
     3541 tgcgggggct ccacgcaggc cccgggccga agatctcgat cttccggcgc gccgactcga
     3601 gcgacgcggc cgcaatccgc tggggctcgg ttcggtggcg ccgggtcgcc ccgttgaagt
     3661 acatccgcac gctcgtcttc ggcgcgccat cggccagcag cgaccgcccg tcccgttgcc
     3721 cggatcgcgg gattcgcagg acgtccatga cggtcgggac gatgtcgatc gtctcggccg
     3781 gccgatctga gacgcgcccc tcgcgctgcc cgggcagctt gacgagcagc ggcacgttca
     3841 tgatgtcggc gaagttgcgg cggtcgggct tcttgtagtg tccgcccggc tggaagctgg
     3901 ccccgtgatc cgaggtgacg atcaccaggg cgcggtcgaa ctcacccctc gaccgcaggc
     3961 ggtcgaggag cagcccgaga agacgatcgg cggcgcccac ctgcagcagg tgccgctggt
     4021 agttgacggc cacttgccag tcgtcggtca cccactgctc gtcgccgtac agaccgggaa
     4081 ggtggcgcaa cgtcgtcgcg gtctgtcctg agggaaggta gacgaacggc tcgtgcggca
     4141 gcagcacgtg ggcgaagtag agcgtcgggc gttccgcgtc gggttcgatc gtgtcgatcc
     4201 actcgtacgg gtgccggtcc ccgctgttcg acgccttgaa acgatcctgc gcgtcctggt
     4261 gccagaaccc ccgccagtcg tggcgcacgt cgggaaggcc ttccgcgagg tcggcgggca
     4321 ggaggcggtg aaggtaagcg acggaggcat ccaggccggc cgccgccagg cgcctggtca
     4381 gcggcgtcgg atcggggcac agagcgggcg ggcacagccg cgtcatcggc tcctgcacgt
     4441 gcagcgcgta tgcgggcgcc aggccggtga acaggttgtc cgggtgggaa gaggcggtcg
     4501 gcgtctcccc ccgcctcggg tagcgtccgg tcaggatggc ggggaccgcc atctcggtcc
     4561 ggtcggccac gctggtggcc aggcggtacc aggtcgcggt atcggccagc gcggcgaagt
     4621 tggggtacgc cgcgcgatcg aggcctcccc cggcatccat cagcgagacg agcggcaact
     4681 gatcgaacac cacgaggacg acgggaacgt ttaccgccgg cttcgcgtcg ccgaagaaga
     4741 cgctttccac gggccggagc agcctcgagg atggcgacga caagacgaag agcagcggcg
     4801 ccagcaggaa cgcgggaacc agcagagcca cgaacagccg gacgctcgac gaccggcggc
     4861 agcctgcctc cacggcaagg ccagccgcca ttgaggcgag cgccagcccc cagcccggtg
     4921 ccgggaggtg gcggattgcc tgcaggacga cggccgccgc cagcagcgtg cgcagcgttc
     4981 cgacggtaca ggacgcggcc cacccgccga gccgtcccgc cacccaggcg agcaggacga
     5041 agaggagcgg cgcggccacc gacacgagcg ccaggaacac ccacaggtcg cggccttcga
     5101 gccgttgggc cacgaagaac tcagggcttc gccccagcat gtcgagcagc gggtaggaca
     5161 ccgccagggc gaacagggca agaacgtgta cgagcgcgat ccgccggcca acgcccgggc
     5221 tcatcgcgaa gccctcccgc gccccccgcg cgccgaggac agcaggcggt acaggcgcgc
     5281 gggcacgccg ccgagcaggc ggttcaagcc gcgcagcagc ggcgacctcg cgcgcgggtc
     5341 gatcgcagcg agaggcgacc ggcgccgctc gtcgaggaac cgctggacga cagggcaccc
     5401 gaggctggcc aggcagtacg ggctcagctc gccccgcacc aggtgtgaac ggatctcctg
     5461 cagcgcgggg gaattccacg ccgtccgcca ctcggccatc ggcgcgattg gcgtcgagcc
     5521 gtagcagcag gggaggacgc cgcgccgcag gatgtagtag ctctcccacg gctcgcggca
     5581 cagcggttcg ttcgggtagc ctagctcgtc ggtcgcgctc acgccttctt catcctcaca
     5641 gggtcgcggt tggacggaac cgcgacgcct cgtccaggcc gaagtcgaac tggctgacga
     5701 cgctcacctc gtaacgggcg gcgtaccgct cgcacgcggc aaatacgggc tccagcgcct
     5761 cgggcgtcat cagctcgcgc tcgtaatcga accggtagcc gccccgctcg cgcacgatgc
     5821 cggggtgctc caggtgcagc agcggccgca ggacgagcgt gtcggcttcg accagccggc
     5881 acaggcggaa ataggactcg aggtcgtcga ggttggcggg catcggcatg aacaccatgt
     5941 tcagccgcgg ccagcccccc ccggcctcgc gcgcgtcccg gaggaagagg aggccggcaa
     6001 gcacctcgtc ccaccgctcg ttccggaggc gggcgtacgt ggccccgctc gccgcgtcga
     6061 gcgaaacgta caggtggacg cgccggccgg ccagcaggcc aacggttcgc ggcgtgaacg
     6121 cctgaccgtt ggtcgacagc tccatcacct tgccgctcga ggcggcgagg tcgagcaccc
     6181 gatcgagacg ggggtgcagg agcggctcgc cgaacgagca gttgaccagc agccgcgcgc
     6241 cctcgaacag gcctccatag ccctcgagtg tccgatcgtc gaccgtctcg tccaccgcct
     6301 cgccctcgag cgccttcatc gggtcccaga gacagtacac gcaggccggc ttgatgttgc
     6361 accggccgta gaggtcgatg ccgagcgtct gggggaacga ctcgacaacg gtcgcgccac
     6421 gctgcatctc gcgctggttc cgcaccgcgt tggcggcaag gaacacctcg cgttcgtggc
     6481 gccgctcgtc gtcgtggagg gtgagcgccc cgacgcggat gcccagctcg cgcgggtcgc
     6541 cggcatggct cgacgccggc gtcagcacgt tgaccctcag gatgatctcc aggtccccgc
     6601 ctggatcggg cagcgcgacg ctgtaagtgt gccagccctt ggcgagatcg aagcgggcca
     6661 gctcgcggcc cgccgcgctc acggtcacct gctgggcgag attgcgaaac gccgagaaga
     6721 gcggcaggga acagaagcgg cggccgcatc gcaggctgtc gcgcaggagc agggcggcct
     6781 cgcgccgcgt ccacctgaac accccgcgct cgtcccgctc ctcgccgttc cagccttcgc
     6841 cggcggccgg caccccgcag cacacctcac ccgcgctctg ccgggcggca cgatcaacgt
     6901 cctcgacacg cacggtgtgg tggaaggagt tgacgtgcgc gtggcgcctg cgatcgccgt
     6961 gagcctcgat gggcccgacg cgcacgccga gcgcgcgcgg atccccgggg cgggcctcgg
     7021 gaggacagag ggcgctcagg cgcagggtaa gccgcgcaga ggccggctca cccgcgatcg
     7081 attgcgccgc gccatccagc acgtccacat cgtacaagtg ccagccgcga cgcagcggga
     7141 gctcggccag caggcgctcg tcgcgccaga ttgccaggac ctgctgtcca gcgtcggtct
     7201 cggcggcgat cggtagggtg aggaagcgcg tgccgggacg cacgagggcc ggcaccagca
     7261 cgctcgcctc gcggcccatc cagctgaacg gaaagtactc gtgcgtctcg cgctcgtacc
     7321 atccgtcgag ctcgagcggc gagtcgagcg cgtcctcgtc gaaggcgtcc aggcccttca
     7381 ccatcacccc gagcattcgg gggtcgcctg gcaccgagaa ggtgctatcc agccgcagct
     7441 cgacgtcgaa gtcgcgcccc ccgccgagcg gaaaggccaa gtacccgagc tgctccgcca
     7501 cctgggccga accggtcgtc cgcccgttga ccgcgagcga cagcgacggc accgacggcg
     7561 ccgcgcccca gaccccgcac gccaggcgca gccagaccga ccggcacggc gactcggcgg
     7621 caattctcac ccgcgccagg ggtccactcc accggaacgg ccgggcacca tcgtgctcga
     7681 ccgggtacca gccgtcccca tatgtgatgt tgaaggtcgt ctgcatgccg gacgggtcat
     7741 tctagcagga ggtgagagtc tgaggcggga cgcccgtgcc actgggccac tgcgccacac
     7801 aggcgagacg cctgtgctac tgggccactg cgccacacag gcgagacgcc tgtgccactg
     7861 ggccactgcg ccacacaggc gagacgcctg tgctactggg ccactcgagc tgtcgaaggc
     7921 cgcggtgacg ggtgtatcgt ctcaattgat ggtgcgtctc gcggtggtag tgacgctcgt
     7981 gctggctccg gcggcttccg gggccctggc tcaatcgccg gtggaggggc agggcagtgc
     8041 cgcgcaggcg cagggcgccg cacaaccgct ggagtccgaa cgccccgaag cgatcatcga
     8101 ccgcctgccg atctcgctcg aggcgatccg gagcggcatc gagcgcgcgc ccgaggctcc
     8161 gtccgccctc cgcatcggac agctccccac gttccgggtg accatctacg ggcagcgccg
     8221 ccaacttctg ccggacttcc aggaaacgct gcggcagccg tggcaggcgc cgatccccgg
     8281 cggcatccac aacaaggaag tgctcgacat gatcaccccg ccagaggcgc ggagtttcgg
     8341 cgcgttcacc aacggcgacc tcgccgaggt ggccggcacg gcgctggtga gcggcctggc
     8401 ggcccgcgcg ctctacaacg ggtacacggc cgtcaaggga gctttgcgca gctggcagga
     8461 ggagcggatt cgccgggagg tggaggccga gctcgaggcc ttcaagcgcg ccaacgggat
     8521 cgtcgatccc tcgccgcggt cgcagacccc gtccaagcct ccgggcccgg aggaagaaaa
     8581 gaaataaccg tgcggagggg cacgcgatca agcggccagc gtgggtccca ccagcttgac
     8641 gatcgcggcg tgcaggcggt cgggcgcgca cggcttcaac aggacggtgt cgcacccggc
     8701 gttgcgcgcc agcttgaggt gctcgatgtc gctgtccccg gtgagggcga tgattggcgt
     8761 cccgcgcgtc tgcgcccggt tcttcaacag gcgcaccgcc tcccagccgt cgagcttggg
     8821 catccgcagg tccatgacga tggcgtcgtg ccgccccttg atcgccttgt cgaacgcctc
     8881 gaagccctgg ctggcctgct cgacctcgca gccggcaccg gccaggtaca gcgcatacat
     8941 ctcgcgtatc tctgcgctgt catcgacgag gagcacgcgt atctgacgat cggctgacac
     9001 catgcacgca gatccctccg ggggtcaccg gtggtagacc gaatcctatt cgacccgcgt
     9061 gccaagttgc ctccgcgcac gatcgcccgc cccgcacggg atcgcgcggg tgttcgcgac
     9121 tcgtcgagtc cggatttacc gggattccgg cagtgtctcc ggcacttctg acgacaattc
     9181 gccagttgcc gggaggcggt ccaaggctcc tggcggaatc ctggcgcaac cgggacgatc
     9241 gggccgggcc ttcagttggc accgccgttg cgtcttaaga gggcaaggcc cgctggttgt
     9301 gagggtgctt acggatgggg gcccgtaagt gctcgaagca gccggcgggc gttttcgcgt
     9361 acggcgtcag cgggcggcaa gaacctgccg cacttcgtcg acgacgcggc cgctggggca
     9421 gggcttggtg aggacggcgc ggcagccggc cgccaccgcc atctgctgga tgtcttcctg
     9481 ggcgccggtc accgccacga tcgggatctc tcgcgtcgtc gggttcttct tcagccagga
     9541 caccacctcc cagccgtcca gcctgggcat cagcaggtcc atcaggatca cctggggctt
     9601 cagcttgtaa cccttgtcca cggcctcgaa cccgtcggct gcctcctcga cgcgaaaccc
     9661 ggcagcctgc agtgcgtaag aatagaggtt ccggccgtcc gggtcgtcgt ccacgatcaa
     9721 gacgagcggg gaggaatcgg tggcgtccat gatggtccta ctgtggcccg cggggcccgg
     9781 ccgagtcaac tgcgggtata atccgccggt tgcgccggga gaccggctcg gggtgcgccg
     9841 atgatcgtgg cccgcaagct gaccctttcg gggcgcgtgc agcgcgtcgg gttccgctgg
     9901 ttcgccgtgg aggccgccga gcgcgagggc gtcgccgggt gggtggccaa cacgcgcgac
     9961 ggccgggtcg agatcttcgt cgagggcgac gccgaggcgg tcgagcgctt cgagcgcgcg
    10021 gtgcggcggg gaccgatcgc agcccgcgtc gacgaggtcg agaccgaagt acggccgccc
    10081 agcgggcggg tggcggggtt cgaggtgcgg cggtgaatgg gcgcgagggc ccggcctccc
    10141 gccccacagc aggagccatg gacacgctca aggcacggat tcgttctgtt cccgactttc
    10201 cgaaggcggg catcctcttc tacgacatca cgacgctgct cggcgacccg gcggggtttc
    10261 gcgcggcgct cgacgcgctc gcgctgccgt accgcgacca gggcatctcg gtcgtggtcg
    10321 ggatcgagag ccgcgggttc atcctcggcg gggcgctggc cgaccgtctc ggcgccgggt
    10381 tctgcccggt gcgcaagccc ggcaagctgc ccgcccggac gatcagcgag tcgttccagc
    10441 tcgaatacgg gaccgatcgc ctcgagatgc acgaggacgg aatcaagccc gggcagcggg
    10501 tgctgatcgt ggacgacgtg ctggcgaccg gcgggacggc ggcggcaacc gcgcggctgg
    10561 tgcgccggct gggaggcgag ctgcacgggc tcgcgttcct gatcgaactg ctgacgctcg
    10621 acggccggtc gcgcctcgct ggcgagcgcg tcaactcggt gctgaagtac tgatgctaaa
    10681 atgactggtt cgctgttttc gcagcgacgg gtccccgtag ctcagtctgg atagagcgtc
    10741 cgcctcctaa gcggaaggtc ggcagttcga gtctgcccgg ggacgccagc cttcgctcgc
    10801 atggatcaag tagcgagcta cggctaggca ggccagtctc gctcgcatgg atcaagtagc
    10861 gagcgaaggc tggggacagc aagctgcgct cgcctggaac gcgacatgaa gagaatcgaa
    10921 cgacgccgcc tgaaggaaaa cgaggtcgcc ctgtcggtcg cacgtgcgcg ggagaccttc
    10981 gacatctacc gcaagccgat catcgcgggc ctggtgacct tggcgctgat cgtcgtcgcc
    11041 gtggtgggag tcaccgcgtg gcgaaagtcg gccgactcga acgctcgcac gctgctggcc
    11101 gacgcgatgg ccgtcgccgg cgcgccggtg gtgccggcga cgcccggccc caacacaccc
    11161 gccccttcgc ccggcagctt cgggagcgag aaagcgaaac tcgaggccgc gctgcagaag
    11221 ttcatggcgg cggccgcggc tcacccctcc tcggagcccg ggatcgcggc gcgctaccac
    11281 gcggcggcaa tcctcgccac gctcggccgt cacaaggagg cggagcagcg ctaccgcgag
    11341 gtgatcgaaa agccgggcgc gggcctctac ggtcccatgg cgcggctggg cctggcgaac
    11401 acgcaggcgg cggcaggcca gcgcgaccag gcgattgccg cctacaagga actgagcgcc
    11461 cggaaggata gcgacttgcc ggtggacgcc atcctgatgc agctcgcgag gacgtacttg
    11521 caggacggca agaccgccga ggcgcagcag acgctccgcc ggatcggcga cgagttcccg
    11581 cagtcgccgt acgccagcct cgccgcgaag gagctgggac agatcaagaa ctagaccgga
    11641 gagcacgggg ctgaggcccc gtgctacgcc tgtcaaccgg cgaccgggtc aagccccgta
    11701 ctaagcctgt cggaagcggt agctgagtat cttgtagatc agcttcgcgc agaggaagtt
    11761 cggccccacc atccccggca gcgggttcag ctccaccagg tcgcacgcca ccacccgccg
    11821 ccgcgacagc accgccttga gaagcgcggt cgtctctggc caggaaaggc ccccgggctc
    11881 cggcgtgccg accgcgggca tgatcgcggg gtcgaggccg tcggcgtcga tcgtgatgta
    11941 gacggcctcg gacagcccct cgaccacctt ctcaatccag tcgggatcct ggcgcatggc
    12001 gtggtcccag aacacccgcg tccgaagcga cgggatcacg cgcgattcct cccgcgaaag
    12061 gctcctgatc ccaacctgcg tgcaggggac gagctcgacg atgcggcgca tcgcacaggc
    12121 gtggctgtag cggctgccct ggtaagagtc gcgcatgtcg gcgtgcgcgt cgatctggag
    12181 cacggagagg ttcggcgtcg cagccgcgta cgcttcgacc accggcacgc tgatcgagtg
    12241 ctcgccgccg agcacgacga ggaacttctc gtgcgggatc agctccgaga ccacgcgccg
    12301 gatctcgtcg agcgccaccc gcatgtcgtt gtagggcagc tccatctccg gaagcgtgac
    12361 gatgccgcgg ctccaggcgt cggttcccgt ctcctcgtcc cacagctcca cctgccccga
    12421 ggcgagcagg atctcgcgcg ggccgttccg cgtgccggtc acgtagctgg tcgtccgctc
    12481 gaagggaacc ggcaacacga tggcccgcgc gtcgtcgaac gatcgggccg gctcgtagcc
    12541 cccgaacgag agcggggaag gatgttcgta ctcgactggc ggcttcatcg cgtcctccgc
    12601 ggcggggccg gagccccgcg catcgcttgc gtccactccc gattcagaac gaccggccga
    12661 tgcgcacgta cggccggggg tcgaggtgcc ggccggtcgc cccgtcacgc gcccacgcga
    12721 tccccccggt gaaggtcagc ggcaggccaa accccacgac cgtatccagc gacagctcgg
    12781 ccccgaccga cgtcttgaag tccggccacg cgaagggcaa gccagcgccc caggcgtggc
    12841 ccgcgtcggc gaaaaccgcc ccgtggaccg cgcgaaggaa cagcggcgcc gtgcgccagc
    12901 cccgctcgac ccgggcgagc gggaagcggt actcgaggct gccggaaacc acccgcgtgc
    12961 cgacataggc ctcgttctcc aggccgcgca gcatctcgac ggcctcgctc cggacgtcga
    13021 ccaccgatct cgcgggagcc gcgcccccga gaaagaagac gcgcctgaca ttgcggtcgc
    13081 cgctcgagaa ggcgccgccg gccctcgcgg cgagcaccgc gtggcgtccg cccagccggc
    13141 ggaagacacg cgcctgggcg gtccacgcct gcgcatcgcc gtccgcgccg aacgcccgcc
    13201 tgacgtgctc ggttccgatg ccaagcgaga cgccctcctc ctggctgatc gagtacccgt
    13261 accgccgcgc ggtgtccagc ctccatcccg cctcgatggc gtgcctcagg aaggtccttg
    13321 gcgcgtccgc gcgcgtcgcc tgcaacgtgc tcctctcgta cttgaatgcg gcctgccaca
    13381 gctgcaggcg gcggatacgg gcgaacggca gcatcaggcc cgcgctcaca ctccgctcgc
    13441 gcagctccgc gtcgggcagg cgcccccccg actggtttcg cacggccagg aacgacgtct
    13501 cgtcggacgc cgagatccac agcggaaccc gccagcggtc gtaggtgtag ccaaggtcca
    13561 cgtccgggcg gccgcggtgg aggccgttca ccgcgtcccc ttccgacccc acgcgccacc
    13621 gcagcaacgc cccccaggca tgacgcccca gcacgtcgat cccgcccagg gccagcccga
    13681 ctcgcagcgt gtcgccggtg tccgcaaccg gcgtccaggc gcgcggccgc agcgtgtcga
    13741 acggggagta ccctcggtcc tccacttggg cgaggggagc ggcagcggcg ctggcgaccg
    13801 gcgccgggcc ggtccatccc gcatcggccg ccgacgcggg atcgaacggc agcgcgaaca
    13861 ggtcggcgcc ttcttccgtg tatccgacga agaccacgcg ccgcccgtcg ggcgagatcg
    13921 ccggcgaggt cgccccgcca gggaccgccg tcaggcggcg gaccagcccc ccctcgacgg
    13981 gaacggtgta aagctggaac gagcgcgcga gcagcgcgcc gggggccgct cccggcgggt
    14041 ggtcggacgc gaaaacgacg gactctccgc caggcgccca cacgggcgtg accaggcggt
    14101 agagcgcgga ggagacgagc acgcgaatgc tgccggtggc cgggtcggcc accgcaatct
    14161 ccgagggacc gccaggcacc cggcgctcga aggccaccag ccggccgtcg ggtgaccagc
    14221 gcggcgacgc ggcctggccg ccgccgtccg gccccggcct cggctcgagc ctgatcccct
    14281 caccgccctt ccggcgcacg tcgaaccagg ccagctcgcg acggccgccg gcgatccgga
    14341 tggcgaccag cgaggcgccg tcgggtgaga gggagggctg catgagacgg gcgctttcgg
    14401 tcagcgccgc aaccgctccg cctcggggag acaccgcgta gaggtcggcg cggagcgcca
    14461 cgttcgtctc gagctcgagc tgatcgaaga tcaccacccg gtcaccgacg ctgagcccct
    14521 cggcaccgta ctgcgtggcg atctcgcgcg ggatccctgc cgggccctca tcggcgccgt
    14581 gcccacggtc gagccgcatg atggccggga accggtgcgg cgtctgcagc gtgtagaaga
    14641 gtgccgagcc atcaggggcg aaacgcgggc tgccgacgag gaagccgtgg cgggtgaggc
    14701 gggcagggac ggccgggccg gacgccggcg ctcctgatgc cggcagggag gccggcgcta
    14761 ctgaattcat gaagtcgcgc cagaggtcgg gaagcggcac accgagcact gcctcgaagg
    14821 cacccgcccc gaagtagtgg acgcgccccg cctgccgctc gaccagatcg cccagcttcc
    14881 gctcgccgaa ccgcgacacc aggtactggt ggaagaaccc gccgaccagg tacgaaccgt
    14941 ttccgccggg ccagtccacc aggccgccgt tgtagcggtc gatcggctcg gggcccgcct
    15001 cgcgcacgcg ctggcggacg atcgtcgcgg cgtcgccggc cctcacccgg ccttcgccgg
    15061 tctcacgcga ctcgacccag gtggcgaacc cctcgatctg ccacagcggc agcgcgaggt
    15121 tggggaaggc cagcggggag cggccgaaga tgccccgcat caccgaggcc cagccgcgcg
    15181 actggttggt ctgcaggatg tgcgcgtact cgtgcacgaa cacgagccgc agccactcgc
    15241 gcgtgaaccc gatcagttcc gactgcgcgg gccaggccgc ggtgagggcg atcgtgttgt
    15301 acggcacggg gaacgcgctg ccgttcgacg cgtcgtcctg gtcggcaagc acgacgtgcg
    15361 tgaccgccgg agcgggaagg ccgagcctgg ccggcagctc gcgctgaacc gactcggcaa
    15421 tcgcggccac ttcacgcgcc agctgttctc cgccctggtg gtagtagatg cggaagtgag
    15481 gcgtgaccag cacgcggaag cgcaggtgcg gcaggtagcg cgacgcggcg gccgcctcgg
    15541 acgccgcgag cgacagcagc agcgcgagaa gggcgagccg cctcatgact cctgcgcgtc
    15601 gcggcgcagc tcggcgagca gcgtcagcgc ctcgagcggc gtgaggtggt cggggtcaac
    15661 ggcgagcagc cgctccacca gccgctcggc cctcggcgag ggaagcgcct ggaacagccc
    15721 gagctgctgc tgcggctcgg tcggggtgcg gctgagcgag ggacggccgc cgcgcgccag
    15781 ctcgtcgtgc tcgagagcgc tcaggatctc ccgcgcccgc tccaccacct tgggaggcag
    15841 tcccgcgagg cgggccacct ggatgccgta gctgcgatcg gcgcgccccg gaacgacctt
    15901 ccggaggaac aggatctcgt cgtgccactc gcgcgcgtcc acgtggcagt tgacgacccc
    15961 ggggatgccg tcggccaggt cggtcagctc gtggtagtgc gtggcgaaga tcgtcttggg
    16021 gcgcgcgcgg gagttggtgg cgaggtgctc ggccacggcc cacgccaggc tcaggccgtc
    16081 gaaggtcgcg gtcccgcgcc cgatctcgtc gagaatcacc aggctgcgcg aggtggccga
    16141 gtgcaggatg ttcgccgtct cctgcatctc caccatgaag gtcgactggc cgcgggcgat
    16201 gttgtcggac gcgcccaccc gcgcgaagat gcgatcgacc agcgcgagct tggcttcgcg
    16261 cgccgggacg aaggagccga cctgcgccag caggcacacg agcgccgtct gccgcaggta
    16321 ggtggacttt ccccccatgt tcgggccggt caggacgatc agctggtggc ccgagccgtc
    16381 gagcagggtg tcgttcggca cgaaggggtc gcgcgaacgc cgctccacga ccgggtgccg
    16441 cccctcggtg atgacgatct cgtcggcgtc gtgaacgtgg ggcttcgtgt agttgttgac
    16501 ggtcgccacc tcggcaagc
//
LOCUS       DAOPQX010000042        16458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4200_length_16458_cov_4.470158, whole genome shotgun sequence.
ACCESSION   DAOPQX010000042 DAOPQX010000000
VERSION     DAOPQX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4200_length_16458_cov_4.470158"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKK95_03880"
     CDS             <1..306
                     /locus_tag="PKK95_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC17380.1"
                     /translation="RGLDVNLPASRRAQQLTEERVFVSVPYSFREDHRVRLNNDVVRL
                     EVLQERIRQVMQNRTSREVFLQSDAGLTVQELFDVMDRLKAGGVEKVGLVARLPEER"
     gene            311..1108
                     /locus_tag="PKK95_03885"
     CDS             311..1108
                     /locus_tag="PKK95_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOC17381.1"
                     /translation="MDSVTAIVLERAREPRSMRPMVAASIALHVLALALLMVRPDPFS
                     SENEDAARSLMTISLGNGGGPDAGGANTLGARPVQRAAAQPEPLRAPQAPAARTPEMV
                     LPSRVVKPRPGAKPAPERPAVRNAPEEAAGRTPTTGPEERFGSAMAETGGYEFGSGLT
                     SGGGGGTGGYLDVGNFCCPDYLITMRRLIQSNWNARQGVEGSVVVKFVIQRDGRLTDV
                     EVEERTGLPALDLTAERAIVMTRQLPPLPAPFPDRSLTVHLRFNYRR"
     gene            1108..2484
                     /locus_tag="PKK95_03890"
     CDS             1108..2484
                     /locus_tag="PKK95_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17382.1"
                     /translation="MLRTNLLAAGIAAALGTAVLLAGQAPPAQQPPQEPRPTQEIALT
                     ITGEPGTLPRYAVPDFIAASPEADTLAAARVIGQVLWDDLAFEREFDLIPRDTYQSIP
                     AARSLTDVPFDRWRELGADGLVIGSVRRVGSDLRVEVRLLQVSTRQSALSKEYTGATA
                     NPRLFAHTIADDIHQNQVGLRGVARTRLTFSSDRDGERLGGTVEERTVKEIYISDYDG
                     ANQRRVTTNRRLNITPTWSPDARSIAYTSYRRGVPDLFISNIYQGTLENPTKGRGQNF
                     LPAWSPDGTRLAFMSDRDGNPEIYVMNRDGSGLRRLTNHPRNDVTPTWSPAGTHVAFT
                     SDRGGSPQIYVVAVDGTGTRRITAESYCDRPSWSPAPYNEIAYASRTGSGFDVKIIDL
                     STGERRQVTFGEGIINESPVYAPNGRHLAFMSNRTGRQQIWTVDRMGRNLRQITTVGN
                     NTMPDWSR"
     gene            2546..3127
                     /gene="pal"
                     /locus_tag="PKK95_03895"
     CDS             2546..3127
                     /gene="pal"
                     /locus_tag="PKK95_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOC17383.1"
                     /translation="MRTRWRMALLVALLVAAALGAGACRKKTPPVARPVMPPPAATTT
                     PARPPAPPEPVGESPVVAEPAMAEDTMASRTLDELNRDSPLRPVFFAYDSAEISPEAQ
                     QALNANAEVLKKYQSWVVTIEGHCDERGTAEYNLALGERRAVAARAYLVSLGIPAERL
                     KVISYGKEFPFDPGQTEEAYAKNRRGHFVITSK"
     gene            3147..4022
                     /gene="ybgF"
                     /locus_tag="PKK95_03900"
     CDS             3147..4022
                     /gene="ybgF"
                     /locus_tag="PKK95_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOC17384.1"
                     /translation="MARTLLASIAALSLLSAGAAPALAASKEQQQMMADIRMLQAQTQ
                     ALQLQLAALTEALKTVSAKIDEQGGATRKAFADQKVLVDAISGDLRVVREKVDDNNLR
                     IGSVSLEIEALRNALPQPGALIAPAPGAEAQPPDAATDPAAPAAPAPVAPAQPPASGM
                     SPQRLYDMAWADYAGGQYSLAIRGFEEYIKSFPKSELAGDAQFFIGETYYVQGQYREA
                     VAAYDQVLASYPASKKVPDTYFKRGLALNALGQTDRARESWEYVLKNFPNSDAGRLAR
                     QRLDQLQRKEEDREE"
     gene            complement(4132..4554)
                     /locus_tag="PKK95_03905"
     CDS             complement(4132..4554)
                     /locus_tag="PKK95_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOC17385.1"
                     /translation="MNRVILVGNLGADAELRYTGGGAAVANFRLATTEVYKDKDGQRQ
                     EQTEWHRVSLWGKQAETIAQWLVKGKQVYVEGRLQTRKWQDKDGNDRYSTEVRGDRVV
                     LLGGPGGGGGRREQADRGDLHDAQQGPAEMPAEDEVPF"
     gene            complement(4733..5098)
                     /locus_tag="PKK95_03910"
     CDS             complement(4733..5098)
                     /locus_tag="PKK95_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOC17386.1"
                     /translation="MATSEITRVEKPWGYELHWAKTDRYVGKILHIRAGHALSLQFHR
                     KKDETILLWSGRMRFEIDRGNGPQHLDLEPGQSVHVAPGDVHRMTAIEDCDVFEVSTP
                     ELDDVVRLEDRYGRRDHGV"
     gene            complement(5085..6896)
                     /gene="ptsP"
                     /locus_tag="PKK95_03915"
     CDS             complement(5085..6896)
                     /gene="ptsP"
                     /locus_tag="PKK95_03915"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOC17387.1"
                     /translation="MHRLTGIGVSPGIAAGHAVLLMESPLVIRFPIAPERVGEELARL
                     DEARERSRRQLLEIKARVARSAGAELAYLFEAQLLMIEDPMLINRVRHLVEEQRVNAE
                     WAVQHAFEELAGVFDGIEDAYLSERKGDVADVAGRLRMNLARARGRGVELFRDVGPGS
                     VLVADDLPPSLAAQVDWSKIQGFAGDTGSRTHHTAILARSLRVPAVLGLGTASRDVPP
                     GALVVIDGTTGELIVDPPPELVARAHARSARPAGRSRRAAAPEAAVTRDGVPIAIQAN
                     IELPGDVQAAREAGAAGIGLFRSEYLLATASPETIGEDQQYEAYRSLVEGMAPGAVTV
                     RTFDVGEAQLQPWLRTSGRRASDRPAQVRPGGPLGLRAIRLSLERREMFKTQLRALLR
                     AARHGRLRIIFPFVSGLEELHEAKAVVAEAAREIEERGGGRVALPPIGVMIEIPSAAL
                     TADLLAREADFFSIGTNDLIQYALAVDRTDAGVSKLYEPLHPAVLRMLRTLVRAAGRR
                     RVPVSLCGEMGADPVILPLLVGLGLTDFSMSPSAIPAARDVIRATSASDARRLAGRVL
                     RLGTTAEIEQCLKECGRQADGGRDTTKGTQGGARGDE"
     gene            complement(6884..7162)
                     /locus_tag="PKK95_03920"
     CDS             complement(6884..7162)
                     /locus_tag="PKK95_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOC17388.1"
                     /translation="MRSQTVTVRNQLGLHARAAARFVHLASRYQSRIRIGRGQRVMDG
                     KSIMGVLLLAASRGTEVTISADGPDEAEALEALCELVESGFGEAPCTA"
     gene            complement(7165..7572)
                     /locus_tag="PKK95_03925"
     CDS             complement(7165..7572)
                     /locus_tag="PKK95_03925"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOC17389.1"
                     /translation="MIGVVVVTHGQLATELLNAAETIVGDLPRFAAVSIGWHDDVDDA
                     REEIRQAIGRVQGPGGVLILTDMFGGTPSNLGLTFLEADKVEVITGVNLPMLIKLAGL
                     EGESKLLEVARQMREHGRNAIWVASDLLRSEGA"
     gene            complement(7626..8534)
                     /gene="rapZ"
                     /locus_tag="PKK95_03930"
     CDS             complement(7626..8534)
                     /gene="rapZ"
                     /locus_tag="PKK95_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOC17390.1"
                     /translation="MSALARPAPRPGAARFIVLTGMSGAGKSQAIRALEDLGYFCVDN
                     LPIALIPTLAELSQRAGSEIPRVAIVVDVREGAFLSRFPKALRSLRAKRHLAPALIFL
                     EASDEALVRRFSETRRPHPLAHDRPVLDGIREERDRLEPIRQLADRIVDTSNLTVHEL
                     RDAFMEWSRGRAAARGLLLTFLSFGFKHGVPAESDLVFDVRFLPNPHFVPELRAKTGK
                     DRLVVQYMEEHDSTGEFLAKLQDFLRFVVPHYVAEGKSYLTVAIGCTGGRHRSVMIAE
                     TLRRTLADLEGVRLRVRHRDIDVGGE"
     gene            complement(8531..9541)
                     /gene="hprK"
                     /locus_tag="PKK95_03935"
     CDS             complement(8531..9541)
                     /gene="hprK"
                     /locus_tag="PKK95_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOC17391.1"
                     /translation="MATLNPGVGIGALLRSRPELFGLPLELAAGAGGLDRRITSPHVQ
                     KVGLALAGFDAYLSPGRLLIFGESEIRYIEGLPPSNRVDALQRTLARDIPAVLVTGGL
                     AAPPELSIESERARVPLLVTPVVTPLAIAKVTAILEDWLAERTIVHAVLMDILGLGVL
                     IVGDSGIGKSECALDLVVRGHRLVADDTVEVRRRAESILIGTCPELTRHHMEVRGLGV
                     INIRELFGVASTRSSKRVELVVQLERWEAGRDYERLGLDQAHYEILGLRVPLLRMPVA
                     PGRNVAILVEVAARNQLLRARGHHAAQRLAARLERRLRNPEGAGPEADVEAGDRDEEG
                     ER"
     gene            complement(9564..10112)
                     /gene="raiA"
                     /locus_tag="PKK95_03940"
     CDS             complement(9564..10112)
                     /gene="raiA"
                     /locus_tag="PKK95_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOC17392.1"
                     /translation="MRLELTGRQVEITPILRKKVEKKLAKLDRLLKGGIVSVQAVLAQ
                     EKYMRRAEITVHTRGEQFLHGISASNDWETSINDCVEKIVQQLQKVKGKWQERKRRAT
                     SVRTLPPAPPDGTVVPEPRRRPRLRRVPRYPVKPMSVDEAALAVEGGDDAFLVFRNAA
                     TDAINVVYRRKDGNLGLIEPEN"
     gene            complement(10123..11547)
                     /gene="rpoN"
                     /locus_tag="PKK95_03945"
     CDS             complement(10123..11547)
                     /gene="rpoN"
                     /locus_tag="PKK95_03945"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOC17393.1"
                     /translation="MAIQQKLHTKLVQKLILTPSLQQAIKLLPMSTLELGELLTQEMV
                     ENPLLEEIPAEDLQPDQAAAVEKPEEPPKEQKADTWDDQDYEYFFGDYLDDGYRPRAP
                     QEVKELPPIENTLSTSTSLSDHLLWQLSIKSDDEVSRGIGTAIIGNLDDDGYLVASVD
                     EIAAMGGWAPDEVERVLRLVQSFDPIGVAARDLQECLWLQIKHLGLEGTPTERIVTEH
                     LRLLQNHQVPELARKLGVGLEELKQHIAVIQHLDPKPGSRYNHPQSQYVTPDVTVVKI
                     EDEYVVVLNEEGLPQLRISPAYRRLLDKGAENSDETRAYVKDKFRSALWLIKSVEQRQ
                     KTIYKVARSIVNFQRDFLDHGIEHLRPLVLRDVANDIGMHESTVSRVVTNKYMHTPQG
                     VFEMKFFFHSGISSSYGESVSSVTIKQRIRKIIEGEDPHKPLSDSKIVGLLQKEGLVL
                     ARRTIAKYREELKIPTSNQRKVLY"
     gene            complement(11699..12424)
                     /gene="lptB"
                     /locus_tag="PKK95_03950"
     CDS             complement(11699..12424)
                     /gene="lptB"
                     /locus_tag="PKK95_03950"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOC17394.1"
                     /translation="MATLRTLELTKSYGGRTVVRGVSLEVASGEVVGLLGPNGAGKTT
                     TFYMTVGLTPPDSGRVLLDGHDVTDDPMYIRARKGIGYLPQEPSVFRGLTVEQNILAI
                     LETLGLDAATRRARLRELLAELNLTSLASAPAYTLSGGERRRVEITRALVISPLFILL
                     DEPFAGIDPIAVADIQRIIFHLRERGIGVLITDHNVRETLRITDRAYIVHDGTIFRSG
                     TPGSLAGDEDVRRIYLGTDFRLD"
     gene            complement(12425..14641)
                     /locus_tag="PKK95_03955"
     CDS             complement(12425..14641)
                     /locus_tag="PKK95_03955"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOC17395.1"
                     /translation="MTRWQKRLRLGLAVFVLLVAATVFFSLRRTPPASPPAPPARAED
                     PNAVAEFARGTLTQAKGTDQEFELRYERLVTYKDGSSRLYGVNAAVPERDGRSFTIAG
                     KEATAAGDEGDVALRGDVELRSSDGLVLLADEALYSAADGSVRIPGRARFSRGRLSGE
                     SIGLAYDREADQVSLLKQVHLEAAAGAAGEETGTIESASALLARRDHILRFEGGARIE
                     RGAQVMIADTVVAHLTEDEEAVRQLQLRGRSSVTGGAKDSSGFDEMSARDIDLVYAED
                     GQSLEQALLRGGGAVVLRSAASKARRRLEGETIDIDMGADGTTVIGLGARGNVRLELP
                     AEGDTPARVITADVLAAVGPPDKGLTTASFRRNVTFAETPSAKDAVARTGTASRLDLQ
                     LAGGFDTIRSADFSGNTRFEEGSLVATSANARYEVEKGVLHLEPGKGRRAARPSVVDD
                     QATIEADRIDVNLDGQHVEATGSVKSDLRPRREAEGTPEAGAGSPGPRTPARLPAMMK
                     QDEPVFGTSDALAYEGDAGRAVYTGRARLWQGDTSIKGERVTLDDRTGDLAASGNVVS
                     TMRLEQVNEKTKQTEMVLSMATADDLVYEEERRRARYTGNARLNGPQGDLSAPVIDIF
                     LLEGGSQVEHLEAYPPPSVTGPAAKAPAAAPAAAPVSITTPDGRRATGLRLRYQSEGE
                     QYEMVGEPVRIVEECRETTGKTLTFFRSADRIVVDGNEQTRTEVKGSSGCSAPRLD"
     gene            complement(14638..16398)
                     /gene="recJ"
                     /locus_tag="PKK95_03960"
     CDS             complement(14638..16398)
                     /gene="recJ"
                     /locus_tag="PKK95_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOC17396.1"
                     /translation="MPTLLWETVPCDEAAVAPLADALGVTRVAARLLCQRGLADPETA
                     ARFLAPSLAQLHDPFRLAGMREAADRIEAAVARREPIAIHGDYDVDGITATVILRRML
                     EGIGGDVVHVLPDRLKDGYGLQAPAVERLHQSGVRLLISVDCGIRSAAAACRARELGL
                     DLIVTDHHEPDEELPPALAVINPKRHDCPYPDKHLAGAGVALKLVQALCQRTGRDAWL
                     PAFVKMASIGTLADVVPLIGENRVIASAGLALLSRGPHKVGLQALLEVAGLAGKRIAS
                     HDVSFLIAPRLNAAGRMASPEVAARLLLATGEAASDEARALAAQLDDENARRQAQEQE
                     ILAAARKAIESDPDIGARSVLVVAGEGWHRGVIGIVASKLVEAFCRPAVVIAIEDGVA
                     HGSCRSIPAFDMLGALTACHQHLERYGGHRAAAGLTLDAAGVKAFRAAIQQYGDDHLG
                     PDDLRPRLRVDAALGFADIRGRLVEELRRFEPFGLGNPRPVFTAAGVQIVDGPRRVKE
                     RHLKFAARHQGRVFRVMAWRAAERERELANRASAFDLAFSLTENTFNGETFTELTMAD
                     ARAAAPPAARPPVEASQGLA"
BASE COUNT         2283 a         5899 c         5839 g         2437 t
ORIGIN      
        1 cgcgggctcg acgtcaacct gccggcgtcg cggcgggcgc agcagctgac cgaggagcgg
       61 gtgttcgtct cggtgccgta ctcgttccga gaggatcacc gcgttcgctt gaacaacgac
      121 gtcgtgcggc tcgaggtgct gcaggagcgc atccggcagg tgatgcagaa ccggacgagc
      181 cgcgaggtct ttctccagag cgacgcgggg ctgaccgtgc aggagctgtt cgacgtgatg
      241 gaccggctga aggccggcgg cgtcgagaag gtcgggctgg tggcgcggct gcccgaggag
      301 cggtagcgcg gtggacagcg tcacggccat cgtgctcgag cgggcgcgcg agccgcgcag
      361 catgcggccg atggtggccg cctcgatcgc cctgcacgtg ctggcgctgg cgctgctgat
      421 ggtcaggccc gacccgttca gcagcgagaa cgaggacgcg gcccggtcgc tgatgacgat
      481 cagcctcggc aacggcggcg ggcccgacgc cgggggcgcc aacacgctcg gcgcgcggcc
      541 ggtgcagcgc gcggcggcgc agcccgagcc gctgcgggcg ccgcaggcgc cggcggcccg
      601 cacgccggag atggtgctgc cctcgcgggt ggtgaagccg cggcccggcg cgaagccggc
      661 gcccgagcgg cccgcggtcc ggaacgcgcc cgaggaggcg gcgggccgca cgccgacgac
      721 ggggccggag gagcggttcg ggtcggccat ggccgagacc ggcgggtacg agttcggctc
      781 ggggctgacc agcggcggcg gcggcgggac cggcggatac ctcgacgtgg gcaacttctg
      841 ctgccccgac tacctgatca cgatgcgccg cctgatccag tcgaactgga acgcgcgcca
      901 gggtgtcgag ggcagcgtgg tcgtgaagtt cgtcatccag cgcgacggcc gcctcaccga
      961 cgtcgaggtc gaggagcgaa cgggcctgcc cgccctggac ctgacggccg agcgcgccat
     1021 cgtgatgacg cggcagctgc ccccgctgcc ggcaccgttc ccggatcgtt cgctcaccgt
     1081 gcacctgcgg ttcaactacc gacgctgatg ctccgaacca acctcctcgc cgccgggatt
     1141 gcggcggccc tcggcaccgc ggtgctgctg gccgggcagg cgccgcctgc gcagcagccg
     1201 ccgcaggagc cgaggcccac gcaggaaatc gcgctcacca tcacgggcga gcccgggacg
     1261 ctcccccgct acgcggtgcc cgacttcatt gcggcgtcgc cggaggccga caccctcgcc
     1321 gcggcccggg tgatcggcca ggtgctgtgg gacgacctcg ccttcgagcg cgagttcgac
     1381 ctgattcccc gcgacaccta ccagtcgatt cccgcggcgc ggtcgctgac cgacgtgccc
     1441 ttcgaccgct ggcgcgagct gggggccgac ggcctggtga tcgggtcggt ccggcgcgtc
     1501 gggagcgact tacgcgtcga ggtgcgcctg ctgcaggtgt ccacgcggca gtcggcgctc
     1561 tccaaggagt acacgggggc aacagccaac ccccggctct tcgcccacac gatcgccgac
     1621 gacatccacc agaaccaggt ggggctgcgg ggcgtggcgc gcacgcggct gaccttctcg
     1681 agcgatcgcg acggcgagcg gctcgggggg accgtcgagg agcggacggt caaggagatc
     1741 tacatcagcg actacgacgg cgccaaccag cggcgcgtca cgaccaaccg gcgcctcaac
     1801 atcacgccga cctggtcgcc cgacgcccgc tcgattgcct acacgtcgta ccggcgcggc
     1861 gttcccgatt tgttcatctc gaacatctac caggggacgc tcgagaaccc cacgaagggc
     1921 cgcggccaga acttcctgcc ggcctggtcg cccgacggga cgcgcctggc cttcatgtcc
     1981 gaccgcgacg gcaacccgga gatctacgtg atgaaccgcg acgggtcggg gttgcggcgg
     2041 ctgaccaacc acccccgcaa cgacgtgacg cccacctggt cgccggccgg gacgcacgtc
     2101 gccttcacgt ccgaccgcgg cggctctccg cagatctacg tggtggcggt ggacgggacc
     2161 gggacccggc ggattaccgc cgagtcgtac tgcgaccgcc ccagctggtc gcccgccccg
     2221 tacaacgaga tcgcctacgc ctcgcggacc ggctcggggt tcgacgtgaa gatcatcgat
     2281 ctgtcgaccg gcgagcggcg gcaggtgacc ttcggcgagg gcatcatcaa cgagagcccc
     2341 gtgtacgccc cgaacgggcg gcacctcgcc ttcatgtcga accggaccgg cagacagcag
     2401 atctggaccg tggaccgcat ggggcgcaac ctcaggcaga ttacgacggt cggcaacaac
     2461 acgatgcccg actggtctcg gtgaagccgc gcgggaagcc cgcgcccgta cagtcagaac
     2521 cgagaaggag aagttggaga aggcgatgcg aactcgatgg cggatggctc tactcgttgc
     2581 gcttctggtg gcggcagccc ttggcgcggg ggcgtgccgc aagaagaccc cgccggtggc
     2641 ccgcccggtg atgccgccgc cggcggcgac gacgacgccc gcgcggccgc ccgcgccgcc
     2701 cgaaccggtc ggcgagagcc cggtggtggc cgagccggcg atggctgagg acacgatggc
     2761 ctcgcgcacg ctcgacgagc tgaaccgcga ttccccgctg cggccggtct tcttcgccta
     2821 cgacagcgcg gagatctcgc ccgaggcgca gcaggcgctc aacgcgaatg ccgaggtgct
     2881 gaagaagtac cagtcctggg tggtgaccat cgaggggcac tgcgacgagc gcggcacggc
     2941 ggagtacaac ctcgcgctcg gcgagcgccg ggccgtggcc gcgcgcgcct acctcgtgtc
     3001 gctggggatt cccgccgagc ggctgaaggt gatcagctac ggcaaggagt tccccttcga
     3061 tcccggccag accgaggagg cgtacgccaa gaaccggcgc ggccacttcg tgatcacgtc
     3121 gaaatagccc gagcgggaga actccaatgg ctcgcacact cctggcgtcc atcgccgcgc
     3181 tctcgctgtt gtcggccggg gcggcccccg ccctcgcggc cagcaaggaa cagcagcaga
     3241 tgatggccga catccggatg ctgcaggcgc agacgcaggc cctccagctg cagctcgccg
     3301 cgctgaccga ggcgctcaag accgtgtcgg cgaagatcga cgagcagggc ggcgccacgc
     3361 gcaaggcgtt cgccgaccag aaggtgctgg tggatgcgat ctccggcgac ctgcgggtgg
     3421 tgcgggagaa ggtggacgac aacaacctgc gcatcgggtc cgtgtcgctc gagatcgagg
     3481 cgctccgcaa cgcccttccg cagccgggtg cgctgatcgc cccggcgccc ggcgccgagg
     3541 cgcagccgcc cgacgcggcg acggatccgg cggcccctgc cgcgccggcg cccgttgccc
     3601 cggcgcagcc gcctgcctcg ggcatgtcgc cgcagcggct gtacgacatg gcgtgggccg
     3661 actacgcggg cgggcagtac agcctggcca tccgcgggtt cgaggagtac atcaagagct
     3721 tcccgaagtc ggagctggcc ggcgacgcgc agttcttcat cggtgagacg tactacgtgc
     3781 agggccagta ccgcgaggcc gtcgcggcgt acgaccaggt gctcgcaagc tatccggcga
     3841 gcaagaaggt ccccgacacc tacttcaagc gtgggctggc gctgaacgcg ctcgggcaga
     3901 ccgaccgcgc gagggagagc tgggagtacg tgctgaagaa cttcccgaac tccgacgcag
     3961 ggcgcctcgc ccgccagcgg ctcgaccagc tgcagaggaa ggaggaagac cgggaggagt
     4021 gaggcagccg gggccactgc cgcaagaacg gggcttcagc cccgttcttg taccacgcgg
     4081 cttcaacccc atcctgcggg agcgaccctg gtcaccaggc gagctccggg gttagaacgg
     4141 cacctcgtct tccgccggca tctcggccgg gccctgctgc gcgtcgtgca ggtcgccacg
     4201 gtcggcctgc tcgcgccgcc cgccgccccc gccgggcccg cccagcagca ccacgcgatc
     4261 gccccggacc tcggtgctgt accgatcgtt gccgtccttg tcctgccact tgcgcgtctg
     4321 cagccggccc tcgacgtaga cctgcttgcc cttgaccagc cactgcgcga tcgtctcggc
     4381 ctgctttccc cacaggctca cgcggtgcca ctcggtctgc tcctggcgct ggccgtcctt
     4441 gtccttgtag acttcggtcg tcgccagccg gaagttggcg accgcggcgc cgccgccggt
     4501 gtaccgcagc tcggcgtcgg cgccgaggtt gccgaccagg atcacgcggt tcacgctgcc
     4561 cattgcatcg ttcctttcaa ttgatccgcc acgcgtccat tctaggccaa gcctgcggcc
     4621 gcgtcgacgg tggcagttcc tgcgatcaga gggcggacac gaactgcaac gccgtcgtgc
     4681 ccgccaactg aagatcgatc gggtgcgttg acgtgctcac accggccaga gactacactc
     4741 cgtgatcccg cctgccgtac cggtcctcga gccgcacgac gtcgtccagt tccggggtgg
     4801 agacctcgaa gacgtcgcag tcctcgatcg cggtcatccg gtggacgtcg cccggcgcga
     4861 cgtgcaccga ctgccctggc tcgaggtcga ggtgctgcgg gccgttgccg cggtcgatct
     4921 cgaaccgcat gcggcccgac cagagcagga tcgtctcgtc cttcttgcgg tggaactgca
     4981 ggctgagcgc gtgcccggcc cggatgtgca ggatcttccc gacgtaccga tcggtcttcg
     5041 cccagtgcag ctcgtacccc cagggcttct ccacccgggt gatctcactc gtcgccacgc
     5101 gcgcctccct gcgttccctt cgtggtgtcc cgcccgccgt ccgcctgccg cccgcactcc
     5161 ttgaggcatt gctcgatctc cgcggtcgtg cccaggcgca gcacgcgccc cgcgagccgg
     5221 cgcgcgtcgg acgcgctggt ggcgcggatc acgtcgcggg cggcggggat ggccgagggg
     5281 ctcatgctga agtcggtcag gccgaggccg acgagcagcg gcaggatgac cgggtcggcc
     5341 cccatctcgc cgcagagcga gaccgggacc cgccggcgcc cggccgcgcg gaccagcgtg
     5401 cgcagcatcc ggagcacggc cgggtgcagc ggttcgtaca gcttcgacac gccggcgtcg
     5461 gtccggtcga cggccagcgc gtactggatc aggtcgttgg tgccgatgct gaagaagtcg
     5521 gcctcgcgcg cgagcaggtc ggccgtgagg gcggccgagg ggatctcgat catgacgccg
     5581 atcggcggca gggcgacccg tccaccgccg cgctcctcga tctcgcgggc cgcctctgcc
     5641 acgactgcct tcgcctcgtg cagctcctcc agccccgaca cgaacgggaa gatgatacgg
     5701 aggcgcccgt ggcgtgcggc gcgcagcagg gcgcgcagct gcgtcttgaa catctcgcgg
     5761 cgctcgaggc tcaggcggat cgcgcgcagg cccagtggtc cgccgggccg cacctgggcc
     5821 gggcggtcgc tggcgcgccg gcccgacgtg cgcagccagg gctggagctg cgcttcaccg
     5881 acgtcgaagg tgcgcacggt caccgccccc ggcgccattc cctccaccag cgagcggtac
     5941 gcctcgtact gctggtcctc tccgatcgtc tcgggcgacg cggtggcgag caggtactcc
     6001 gagcggaaca ggccgatccc ggcggcgccg gcctcgcgtg ccgcctgcac gtcgccgggc
     6061 agctcgatgt tcgcctggat ggcgatcggc acgccgtccc gggtgacggc ggcctcgggg
     6121 gcggccgcac ggcgggaccg gccggcgggc cgtgccgatc gggcgtgggc gcgggccacc
     6181 agctcgggcg gcgggtcgac gatcagttcg ccggtggtgc cgtcgatcac gaccagggcg
     6241 ccgggcggca cgtcgcggct ggcggtgccg agcccgagga cggccggcac ccggagcgac
     6301 cgggccagga tggccgtgtg gtgcgtgcgc gagccggtgt ccccggcgaa gccctggatc
     6361 ttgctccagt ccacctgcgc cgcgagcgac ggcggcaggt cgtcggcgac cagcaccgag
     6421 ccgggtccga cgtcgcggaa caactccaca ccgcgcccgc gggcacgcgc caggttcatg
     6481 cgcaggcggc ccgcgacgtc ggcgacgtcg cccttccgct cgctgaggta cgcgtcctcg
     6541 atcccgtcga acacgccggc cagctcctcg aaggcgtgct gcacggccca ctcggcgttg
     6601 acgcgctgct cctcgaccag gtggcggacc ctgttgatga gcatcgggtc ctcgatcatc
     6661 agcagctgcg cctcgaacag gtaggccagc tcagcccccg cgctgcgggc aacgcgcgcc
     6721 ttgatctcga gcagctggcg ccgcgagcgt tcgcgggcct cgtcgaggcg cgccagctcc
     6781 tcccccactc gctcgggcgc gatcgggaag cggatgacga gcgggctctc catgaggagc
     6841 accgcgtgcc cggcggcaat gccgggcgac acgccgatcc cggtcaggcg gtgcacggcg
     6901 cctccccgaa cccggactcc accagctcgc agagcgcctc gagcgcctcg gcctcgtccg
     6961 ggccgtcggc cgagatcgtc acctcggtgc cgcgcgaggc ggccagcagc agcacgccca
     7021 tgatgctctt gccgtccatc acccgctggc cgcgcccgat gcggatcctc gactggtagc
     7081 ggctcgccag gtggacgaac cgcgccgcgg cccgggcgtg cagcccgagc tggttgcgca
     7141 cggtgaccgt ttgcgaccgc atcgctacgc cccctccgac cgcagcaggt cggaggcgac
     7201 ccagatggcg ttgcggccgt gctcgcgcat ctgccgcgcc acctcgagga gcttcgactc
     7261 gccctcgagg ccggccagct tgatcagcat cgggaggttc accccggtga tcacctcgac
     7321 cttgtcggcc tcgaggaagg tgaggccgag gttggaggga gtcccgccga acatgtcggt
     7381 caggatcagg acgccgccgg ggccctggac gcggccgatc gcctgccgga tctcctcccg
     7441 tgcgtcgtcc acgtcgtcgt gccagccgat cgagaccgcg gcaaaccggg gcaggtcgcc
     7501 gacgatcgtc tccgccgcgt tcagcagctc ggtggccagc tgcccgtgcg tcaccaccac
     7561 cacgccaatc atgttgatcc tcgcctggcc tcgttccccg atccctgatt cctgatccct
     7621 gatccctatt cccccccgac gtcgatgtcc ctgtggcgca cccgcaggcg gaccccctcg
     7681 aggtcggcga gggtgcggcg cagcgtctcg gcgatcatga ccgagcggtg gcggccgccc
     7741 gtgcagccga tcgcgacggt caggtagctc ttgccctcgg ccacgtagtg cgggacgacg
     7801 aaccgaagga agtcctggag cttcgccagg aactcgccgg tcgaatcgtg ctcctccatg
     7861 tactggacca cgaggcggtc cttgccggtc ttcgcgcgca gctcggggac gaagtgcggg
     7921 ttcgggagga accggacgtc gaagacgagg tccgactcgg cgggcacccc gtgcttgaag
     7981 ccgaagctga ggaaggtcag cagcaggccg cgcgcggcgg cgcggccgcg cgaccactcc
     8041 atgaaggcgt cgcgcagctc gtggacggtg aggttggagg tgtcgacgat ccgatcggcc
     8101 agctgccgga tcggctcgag ccggtcgcgc tcctcgcgga tcccgtcgag cacggggcga
     8161 tcgtgggcga gcgggtgcgg ccgacgggtt tcgctgaagc gccggacgag ggcctcgtcg
     8221 gaggcctcga ggaagatcag cgcgggcgcc aggtgccgct tggcgcgcag gctgcgcagc
     8281 gccttgggga accgcgagag gaacgccccc tcgcgcacgt ccacgacgat ggcgacgcgc
     8341 gggatctcgc tgccggcgcg ctgcgacagc tcggcgagcg tcgggatcag ggcgatcggc
     8401 aagttgtcga cgcagaagta gccgaggtcc tcgagggcgc ggatcgcctg cgacttgccg
     8461 gcgcccgaca tgccggtcag cacgatgaac cgtgccgcgc cgggacgcgg agcgggccgc
     8521 gccagcgcgc tcaccgctcc ccctcctcgt cgcggtcccc cgcctcgacg tcggcctcgg
     8581 gcccggcccc ctcggggttc cgcagacggc gctccaggcg cgcggcgagg cgctgcgcgg
     8641 cgtggtggcc acgggcccgc agcagctggt tgcgcgcggc aacctcgacg aggatggcga
     8701 cgttgcggcc cggcgccacc ggcatccgca gcagcggcac gcgcaggccg aggatctcgt
     8761 agtgcgcctg gtcgaggccg aggcgctcgt agtcgcgccc cgcctcccac cgctcgagct
     8821 gcacgaccag ctcgacgcgc ttggacgacc gggtggaggc gacaccgaac agctcgcgga
     8881 tgttgatgac gcccaggccc cgcacctcca tgtggtggcg ggtcagttcg gggcaggtgc
     8941 cgatcaggat gctctcggcg cggcgccgca cctcgaccgt gtcgtcggcg accaggcggt
     9001 ggccgcgcac gaccaggtcg agagcgcact cgctcttgcc gatcccgctg tcgccgacga
     9061 tcagcacgcc cagcccgagg atgtccatca ggacggcgtg gacgatcgtc cgctcggcga
     9121 gccagtcctc gaggatcgcg gtgaccttgg cgatcgccag cggcgtgacg accggcgtga
     9181 cgagcagggg gacgcgggcg cgctcgctct cgatcgacag ctcggggggc gcggccaggc
     9241 cgcccgtgac gagcacggcc gggatgtcgc gcgcgagcgt gcgctgcagg gcgtccaccc
     9301 ggttcgaggg gggcagtcct tcgatgtagc ggatctcgct ctcgccgaag atgagcagtc
     9361 ggcctggcga cagataggcg tcgaagccgg ccagcgcgag gccgaccttc tggacgtgcg
     9421 ggctggtgat gcggcggtcg agccccccgg cgccggctgc cagctcgagc ggcaggccga
     9481 acagctcggg gcggctgcgc agcagggcgc cgatgccgac gcccgggttc agggtggcca
     9541 tgcggagcga cccgcggggg cgatcagttc tccggctcga tcaggccgag gttgccgtcc
     9601 ttccgccggt agacgacgtt gatcgcgtcg gtggcggcgt tgcggaacac gaggaaggcg
     9661 tcgtcgcccc cctcgacggc gagcgccgcc tcgtcgaccg acatcggctt gaccgggtag
     9721 cgcggcacac gccggaggcg cgggcgccgc cggggctcgg gcacgacggt gccgtcgggg
     9781 ggggcggggg gcagcgtccg caccgaggtc gcgcggcgct tgcgctcctg ccacttcccc
     9841 ttcaccttct ggagctgctg cacgatcttc tcgacgcagt cgttgatcga cgtctcccag
     9901 tcgttgctgg ccgagatgcc gtgcaggaac tgctcgcccc gggtgtgcac ggtgatctcg
     9961 gcgcggcgca tgtacttctc ctgcgccaga acggcctgca ccgacacgat cccgcccttg
    10021 agcaggcggt cgagcttcgc gagcttcttc tcgaccttct tgcgcaggat gggggtgatc
    10081 tcgacttgcc gtccggtcag ttcgagtcgc ataggccggc gttcagtaca gcaccttgcg
    10141 ctggttggag gtggggatct tcagctcctc gcggtacttc gcgatggtcc ggcgggcgag
    10201 caccagcccc tccttctgca gcaggccgac gatcttcgag tcgctgagcg gcttgtgcgg
    10261 gtcctcgccc tcgatgatct tccgtatccg ctgcttgatg gtgaccgacg acacgctctc
    10321 gccgtaggag ctgctgatgc cgctgtggaa gaagaacttc atctcgaaca cgccctgcgg
    10381 cgtgtgcatg tacttgttcg tcaccacgcg gctgaccgtc gactcgtgca tgccgatgtc
    10441 gttggcaacg tcgcgcagca cgagcggccg caggtgctcg atcccgtgat cgaggaaatc
    10501 ccgctggaag ttcacgatgc tgcgtgcaac cttgtagatc gtcttctgcc gctgctcgac
    10561 cgacttgatc agccacagcg ccgagcggaa cttgtccttc acgtacgccc gcgtctcgtc
    10621 gctgttctcg gcccccttgt cgaggagccg gcggtaggcg gggctgatcc gcagctgcgg
    10681 cagcccttcc tcgttgagca ccaccacgta ctcgtcctcg atcttcacca ccgtgacgtc
    10741 cggcgtgacg tactgggact gcgggtggtt gtagcggctt cccggcttcg ggtcgaggtg
    10801 ctggatgacc gcgatgtgct gcttcagctc ctccagcccc acgcccagct tgcgcgccag
    10861 ctcgggcacc tggtggttct gcagcaggcg caggtgctcg gtgacgatcc gctcggtcgg
    10921 cgtcccctcg agccccaggt gcttgatctg cagccagagg cactcctgca gatcgcgcgc
    10981 cgccacgccg atcgggtcga acgactgcac cagccgcagc acgcgctcga cctcgtcggg
    11041 cgcccacccg cccatcgccg cgatctcgtc caccgacgcg accaggtacc cgtcgtcgtc
    11101 caggttgccg atgatcgcgg tcccgatccc ccggctgacc tcgtcgtccg acttgatcga
    11161 gagctgccag aggaggtggt cggagaggga cgtcgaggtg gagagcgtgt tctcgatcgg
    11221 cgggagctcc ttgacctcct gcggagcccg cggccggtac ccgtcgtcga ggtagtcgcc
    11281 gaagaagtac tcgtagtcct ggtcgtccca ggtgtccgcc ttctgttcct tggggggctc
    11341 ctccggcttc tcaaccgctg ccgcctggtc gggctggagg tcctcggcgg ggatttcctc
    11401 gagcagcggg ttttcgacca tctcctgcgt cagcagttcg cccagctcga gcgtggacat
    11461 cggcagcagc ttgatcgcct gctggagcga cggcgtgaga atgagcttct ggacgagctt
    11521 cgtgtgaagt ttctgctgaa tcgccatgac cggggatccg tcccaccaat cttagcccgc
    11581 ctcgtgcgcg aacgccatgc gcttgtcgcc cggcgggcga ttgccgcggg gaggcctccc
    11641 ggggggaacc cgggaggcgc tccgcgtttt ccgttcgggc cgcgttcttc agcggccttc
    11701 agtccaaccg gaaatcggtc ccgagataga tgcgtcgcac gtcctcgtcg ccggccaggc
    11761 tcccgggcgt gccgctccgg aagatcgtgc cgtcgtggac gatgtaggcc cgatcggtga
    11821 tccgcagcgt ctcgcggacg ttgtggtcgg tgatcaggac gccgatgccg cgctcgcgca
    11881 ggtggaagat gatccgctgg atgtccgcga ccgcgatcgg atcgatcccc gcgaacggct
    11941 cgtcgagcag gatgaagagc ggggagatga cgagcgcgcg cgtgatctcc acccgccgcc
    12001 gctcaccccc cgacagcgtg tacgcggggg ccgacgcgag cgacgtgagg ttcaactcgg
    12061 ccagcagctc ccgcagccgc gcgcgccgcg tagccgcgtc cagaccgagc gtctcgagga
    12121 tggccaggat gttctgctcg accgtcaggc cgcggaacac cgacggctcc tgcgggaggt
    12181 agccgatccc tttccgcgcc cggatgtaca tcgggtcgtc ggtgacgtcg tgcccgtcca
    12241 gcagcacccg gcccgagtcg ggcggcgtga gaccgacggt catatagaac gtcgtcgtct
    12301 ttcccgcccc gttgggtccg agcaggccga ccacctcgcc cgacgcgacc tcgaggctca
    12361 cgccgcgcac caccgtgcgc ccgccgtagg acttcgtcag ttcgagcgtc cgcagggtgg
    12421 ccattcaatc gagccgaggc gcggagcacc cgctgctgcc cttcacttcg gtgcgtgtct
    12481 gctcgttgcc gtcgacgacg atcctatcag ccgatctgaa gaaagtcaaa gttttgccag
    12541 tagtttcgcg gcattcttcg acgatccgca ccggctcgcc gaccatctcg tactgctcgc
    12601 cctcgctctg gtagcggagc cgcaacccgg tcgcccgccg cccgtcgggc gtggtgatcg
    12661 acacgggtgc cgccgcgggc gccgccgccg gcgccttcgc ggcaggcccg gtgacggagg
    12721 ggggcgggta ggcctcgagg tgctcgacct ggcttccccc ttccaggagg aagatgtcga
    12781 tcacgggcgc cgagaggtcg ccctgtggcc cgttcagccg ggcgtttccc gtgtaccgcg
    12841 cccgcctccg ctcctcctcg tacacgagat cgtcggcggt cgccatcgac agcaccatct
    12901 cggtctgctt cgtcttctcg ttcacctgct cgagccgcat ggtggagacg acgtttcccg
    12961 aggccgcgag gtcgccggtc cggtcgtcga gggtcacgcg ttcgcccttg atcgaagtgt
    13021 ccccctgcca gagccgcgcc cggccggtgt agaccgcccg gccggcgtcg ccctcgtacg
    13081 ccagcgcgtc ggaggtgccg aagaccggct cgtcctgctt catcatcgcc ggcaggcgcg
    13141 ccggggtccg cggccccgga cttccggccc cagcctccgg cgtgccctcc gcctcccgcc
    13201 gaggccggag gtcggacttg acgctcccgg tcgcttccac gtgctgcccg tcgagattga
    13261 cgtcgatgcg gtcggcctcg atcgtcgcct ggtcgtcgac gacactgggc cgggccgccc
    13321 gccgtccctt gccgggctcc aggtggagga cacccttctc cacttcgtac cgggcgttcg
    13381 cggaggtcgc caccaggctt ccctcctcga aacgcgtgtt gcccgagaag tcggcggacc
    13441 ggatggtgtc gaaacctccc gcgagctgaa ggtcgagccg cgaggccgtc ccggtgcggg
    13501 ccaccgcatc cttggcggac ggcgtttccg cgaacgtgac gttgcggcgg aagctggccg
    13561 tggtgagccc cttgtcgggc gggccgacgg ccgccaggac gtcggcggtg atgacgcggg
    13621 ccggcgtatc gccctcggcc ggcagctcga ggcgcacgtt cccccgcgcc ccgaggccga
    13681 tgaccgtcgt cccatccgcc cccatgtcga tgtcgatggt ctccccttcc agccggcgcc
    13741 gggccttcga cgccgcgctc cgcagcacca ccgcgccgcc gccgcgcagc agggcctgct
    13801 cgagcgactg cccgtcctcg gcgtagacga gatcgatgtc gcgggccgac atctcgtcga
    13861 agccggacga gtccttcgcg ccgcccgtca cgctcgaccg gccgcgcagc tggagctgcc
    13921 ggaccgcttc ctcgtcctcg gtcaagtgcg cgacgacggt gtcggcaatc atcacctgcg
    13981 cgccccgctc gatgcgggct ccgccctcga agcgcaggat gtgatcgcgc cgggcgagca
    14041 gtgccgaagc cgactcgatg gtgccggtct cctcgcctgc ggcccctgcg gctgcctcga
    14101 gatggacctg cttcagcagt gaaacctggt cggcctctct gtcgtaggcg aggccgatcg
    14161 attcgcccga gaggcggccc ctgctgaagc gcgcccggcc ggggatgcgg accgacccgt
    14221 cggcggcgct gtagagcgcc tcgtcggcca gcagcaccag cccgtcgctc gagcgcagct
    14281 cgacgtcgcc gcgcagcgcg acgtcgccct cgtcgccggc ggcggtggcc tccttgccgg
    14341 cgatcgtgaa cgaccggccg tctcgctcgg gcaccgcggc gtttacgccg tagagccggc
    14401 tgctgccgtc cttgtaggtg acgagccgct cgtagcgcag ctcgaactcc tgatcggtgc
    14461 ccttggcctg cgtcagcgtt ccccgcgcga actccgccac ggcgttcggg tcttccgcgc
    14521 gggcgggtgg ggccgggggc gaggcgggcg gcgtccgccg cagggagaag aagaccgtgg
    14581 ccgcgaccag cagcacgaac acggccaggc cgaggcgcag ccgcttctgc cacctggtca
    14641 tgcgagaccc tggctcgctt cgaccggggg gcgggcggcg ggcggcgccg cggcgcgcgc
    14701 gtcggccatc gtcagctcgg tgaaggtctc gccgttgaac gtgttctcgg tcagcgagaa
    14761 ggccaggtcg aacgccgacg cgcggttggc cagctcgcgc tcgcgctcgg cggctcgcca
    14821 cgccatgacg cgaaagacgc ggccctggtg ccgcgcggcg aacttcaggt gccgctcctt
    14881 cacccgccgc gggccgtcga cgatctgcac gccggcggca gtgaagaccg ggcgcgggtt
    14941 gccgaggccg aacggctcga agcgtctcag ctcctccacc aggcgtcccc tgatgtcggc
    15001 aaagccgagc gcggcatcga ccctcaggcg cgggcgcagg tcgtcggggc cgaggtggtc
    15061 gtcgccgtac tgctggatgg cggcgcggaa cgccttcact ccggcggcgt cgagcgtgag
    15121 gcccgccgcc gcgcggtgcc cgccgtaccg ctcgaggtgc tggtggcacg cggtcagcgc
    15181 gcccagcatg tcgaaggccg ggatgctgcg gcaggagccg tgcgccacgc cgtcctcgat
    15241 cgcgatcacg acggcgggcc ggcagaacgc ctcgaccagc ttcgaggcca cgatgccgat
    15301 gacgccgcgg tgccagccct cccccgccac caccagcacc gagcgggcgc cgatgtcggg
    15361 gtcggactcg atggccttcc gcgccgccgc gagtatctcc tgctcctgcg cctggcggcg
    15421 cgcgttctca tcgtcgagct gcgcggccag cgcgcgcgcc tcatcggagg cggcctcgcc
    15481 ggtggccagc agcaggcgcg ccgcgacttc cggcgacgcc atccgcccgg cggcgttcag
    15541 cctcggggcg atcaggaagc tgacgtcgtg gctggcgatg cgtttgcccg ccaggcccgc
    15601 cacctcgagc agcgcctgca gccccacctt gtgcgggccg cgcgagagca gggccaggcc
    15661 cgcgctcgcg atcacgcggt tctcgccgat gagcggcacg acgtcggcga gggtgccgat
    15721 cgaggccatc ttcacgaagg ccggcagcca cgcgtcgcgg cccgtccgct ggcagagcgc
    15781 ctggacgagc ttcagcgcca cgccggcgcc ggcgaggtgc ttgtcggggt acgggcagtc
    15841 gtggcgcttc gggttgatga cggcgagcgc cgggggcagc tcctcgtcgg gttcgtggtg
    15901 gtcggtgaca atcaggtcga gacccagctc gcgggcgcgg caggcggccg cggcgctccg
    15961 gatcccgcag tccacggaga tgagcagtcg gacgcccgac tggtgcaacc gctcgacggc
    16021 gggcgcctgc agcccgtagc cgtccttcag ccggtcgggc aggacatgca cgacgtcccc
    16081 gccgatcccc tcgagcatcc ggcgcaggat gacggtggcg gtgatgccgt ccacgtcgta
    16141 gtcgccgtgg atggcgatcg gctcgcggcg ggccaccgcc gcctcgatgc gatcggcggc
    16201 ttccctcatg ccggccaggc ggaaggggtc gtgcagctgc gccagcgagg gggcgaggaa
    16261 ccgcgccgcc gtctcggggt cggccaggcc gcgctggcac agcagccggg ccgcgactct
    16321 cgtcaccccc agcgcgtcgg ccagcggggc gaccgcggcc tcgtcgcacg gaaccgtctc
    16381 ccagagcagg gtgggcatca gcaggttacc gaaatgcaga atgcagaatg cagaatgagg
    16441 caaacggaat gcagttct
//
LOCUS       DAOPQX010000043        16320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4243_length_16320_cov_4.633200, whole genome shotgun sequence.
ACCESSION   DAOPQX010000043 DAOPQX010000000
VERSION     DAOPQX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16320
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4243_length_16320_cov_4.633200"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1127
                     /locus_tag="PKK95_03965"
     CDS             69..1127
                     /locus_tag="PKK95_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17397.1"
                     /translation="MAGTEEARRRLAAGGVIPAHPLALTPERRLDERRQVALTRYYCA
                     AGASGIAVGVHTTQFAIRQPRVGLYLPVLRLAADAIGEHERATGSAIARVAGVCGRTR
                     QAVGEAGAAAGLGYHAALLSLGGWNDASDDELLAHCREVASVMPLIGFYLQPAVGGRA
                     LSEAFWRRFFEIPEVVAVKVAPFDRYRTQEVTRALAAAGREREVTLYTGNDDAIVADL
                     LTDFPGCAGGQRLCFRGGLLGQWAVWTRRAVELVDEIRAERARPGEKTSELLARGADL
                     TDANGALFDARHAFAGCLPGIHEALRRQGLLLGRWCLDEREELSAGQMEEIDRVHARY
                     PGLRDDAFVEAHLEEWLR"
     gene            1205..1993
                     /locus_tag="PKK95_03970"
     CDS             1205..1993
                     /locus_tag="PKK95_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOC17398.1"
                     /translation="MPGTARHRLLVEERRRKILEALEQSERVTVQELVRRFGVSAVTI
                     RKDLDALTEAGAMVRSHGGALRPLGPTDIAIGVKEILHHEEKVRVGTAAAALVQNDET
                     IILDSGTTTLEVARQIAARKFRSLTVITNGLNIAVELARLQNIRLIMLGGLVRHVSHS
                     VVGPHAEQMVRGLNADRLFLGVDGLDLEIGLMTPDVLEAQLNGMLIRVAREVNVVTDS
                     SKFSRRSLSVIARLDAVHRIITDDGIDPAFLAALRARSIEVLTT"
     gene            complement(2112..3035)
                     /locus_tag="PKK95_03975"
     CDS             complement(2112..3035)
                     /locus_tag="PKK95_03975"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOC17399.1"
                     /translation="MSHPRIVIMLVAGVLAFGNGAAQYRGKTAIAHRGASAYAPEHTA
                     AAYRLAIEQGADYVEQDLQITKDGRLVCLHDLTLERTTNVRDLFPGRSRTVEREGKPV
                     TGWHVSDFTLDEIKRLDAGSWFAPKFKGEKVLTFDEAIGLVKGRGGLYPETKAPEVYG
                     RLGFDMERLVIDALGRHGLAEPGADPQTPVFIQSFSAESLKKMRAMGVRLPLLFLVGE
                     NDAETWLTRQRMKEIKAFADGIGPAKGIVSARPAVVVWAHEAGLSVTPYTFRSAGQPA
                     GRTVREEMQQFLYTFGVDALFTDNPDQFPRR"
     gene            complement(3101..3922)
                     /gene="trpA"
                     /locus_tag="PKK95_03980"
     CDS             complement(3101..3922)
                     /gene="trpA"
                     /locus_tag="PKK95_03980"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOC17400.1"
                     /translation="MSRLDSTFSTLRAARQPGLVAFATAGDPDLPRSEAVFLALDAAG
                     ASVLEIGVPFSDPLADGPVIQRASNRALRGGTTLLRAIGLAARVRRQARAPIVLFTYL
                     NPVLRLGVERFAGEAAQAGVDGVLVVDLPLEEAGPFRDAAAGSGLDTILLVAPTSGPE
                     RIRRAAALGGGFLYAVSRLGVTGARERLSDGARALVTRIRAETSLPIALGFGISQPEH
                     VREAARWSDAAVVGSAIVQTVEEHGSDPRLAEAVYDRLTWLLGAASSTGVPPVRS"
     gene            complement(3909..5108)
                     /gene="trpB"
                     /locus_tag="PKK95_03985"
     CDS             complement(3909..5108)
                     /gene="trpB"
                     /locus_tag="PKK95_03985"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOC17401.1"
                     /translation="MTAHAIEWGLRDPDPGGYFGEYGGRFVPETLVEPLEALTRAYDE
                     ARRDPAFTREYLGLLRDYVGRPTALHEARRLAAACGGARIFLKREDLAHTGAHKINNA
                     LGQALLARRMGKSRVVAETGAGQHGVAAAAACALVGLGCEVYMGTDDVARQAPNVARM
                     RLLGAKVIEVGSGSRTLKDAVNEAMRDWVARPHDTHYLLGSALGPHPYPLMVREFQSV
                     IGREARRQLAGATGGLPDAVVACVGGGSNAIGIFDAFLDDASVRLVGVEAGGESLAPC
                     RHAARFAAGSAGVLHGTRTFVLQDEAGNILSTHSISAGLDYPAVGPEHAWLRASGRCE
                     YTTADDHAAAAAFHALARLEGILPALESAHALASACRLAPELGIGRTILVNLSGRGDK
                     DLSNVAT"
     gene            complement(5105..5725)
                     /locus_tag="PKK95_03990"
     CDS             complement(5105..5725)
                     /locus_tag="PKK95_03990"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOC17402.1"
                     /translation="MWLKICGVTREEDARLAASLGADAIGLVFWPHSRRSVDVERARR
                     IAGALPAGVAAVGVFVDEAVERVRLVASRVGLHLVQLHGAETPEACRQMPRPVIKAFG
                     VGPAFDPSLVDAYAEATVLLDADAPESKGGTGTRADWEVAARVARRRRVILSGGLSPE
                     NVAEAIARVAPHGVDVSSGVEAAPGIKDAERMRRFVAAVRQAGGRA"
     gene            complement(5853..6668)
                     /gene="trpC"
                     /locus_tag="PKK95_03995"
     CDS             complement(5853..6668)
                     /gene="trpC"
                     /locus_tag="PKK95_03995"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOC17403.1"
                     /translation="MMGGTRTAASLDDIVLAARRSLEERRRRVPAASMERAALGREPR
                     GEAFAAALRSGPGPAIIAECKRRSPSKGLLRAAYDPAAIARAYERAGAAALSVLTDTP
                     HFGGSIGDLEAVRAATTLPVLRKDFVLDGYQVVEARAAGADAVLLIAAVLGAELGPLA
                     GLARSLGLAALVEVHDEGELVRAVEAGATLVGVNNRNLRTMGVDLETSVRLAGHLPPG
                     VTAVAESGIRTRADAVRLHGCGYHALLVGERLMTAADPGAALQELIGTKDERA"
     gene            complement(6665..7714)
                     /gene="trpD"
                     /locus_tag="PKK95_04000"
     CDS             complement(6665..7714)
                     /gene="trpD"
                     /locus_tag="PKK95_04000"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOC17404.1"
                     /translation="MFAEVLERLGRRHDLTTEQAVASMNAIMEGQATPAEISGLLVGL
                     EMKGPRAAEIAGLARAMREHAVPLPLRSAPGAASGPLVDLCGTGGDRSGTFNISSLAA
                     IVVAACGVRVAKHGNRGVSSACGSADLFEGLGVSLSAPPGIVARCLEAAGIAFLFAPS
                     FHPSMRHAAPVRRELKMRTAFNLLGPLTNPARPSHQLLGVSRPELTGLVARALAALGC
                     RRAWVVHGEGGLDELSTAGYSKVSECRDGAVNSFYVHPSDFGLRPASAEALAGGGVAE
                     NVRIARLVLDGVPGPASDVVAMNAGAALFVAGRATSLEEGAELARRAIGGGDAKRTLA
                     LMARLSHEDGCEEHA"
     gene            complement(7732..8298)
                     /locus_tag="PKK95_04005"
     CDS             complement(7732..8298)
                     /locus_tag="PKK95_04005"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOC17405.1"
                     /translation="MILVVDNYDSFTYNLVQCLGALGARVTVLRNDALDAAGMLALRP
                     SHVVVSPGPGRPESAGATLDLVRGAGSTVPVLGVCLGHQAIGLAFGGRVVRASRPVHG
                     KASLVEHDGAGVFAGLPGPFAAGRYHSLVVADAGLPAELEVTARASGDGAIMGLRHRT
                     LPVHGVQFHPESILTAAGVHLLRNFLAM"
     gene            complement(8309..9775)
                     /gene="trpE"
                     /locus_tag="PKK95_04010"
     CDS             complement(8309..9775)
                     /gene="trpE"
                     /locus_tag="PKK95_04010"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HOC17406.1"
                     /translation="MTMTTFEEFARLAREGSFVPVCKELMADLLTPVSAFLKVAEYSD
                     YSFLLESVEGGEQVARYSFLGKDPLLVLRADSHRTLVEQAGKTTVAGGTIVEELRRVM
                     GEYRAPSVPGLPRFTGGAVGFLGYDAAGWFEPAVRRAGDCPVPAVFMVFDTILAFDHV
                     RHRILVIANARVTPGADLEALYRFACARIGFLERELAREVSTPEPPAASPLVPRPNVS
                     REEFERGVRAVQERIAEGDIYQAVLSQRFDVETSVDPFRVYRALRHINPSPYMFFIRA
                     GRTAIAGGSPEMLVRVEGDRVETHPIAGTRPRGRDEEEDARLAAELASDEKERAEHVM
                     LVDLGRNDLGRVCTYGSVRVAQLMGIERYSHVMHLVSVVEGRLDLGRDRLDALASCFP
                     AGTVSGAPRIRAMQIIEAVEPSRRGVYAGGVGYVDFAGNLDFCIAIRTVVLEPGRASV
                     QAGAGIVADSNPAAEYEESCAKARALLQALEAAASSEW"
     gene            complement(10125..10607)
                     /locus_tag="PKK95_04015"
     CDS             complement(10125..10607)
                     /locus_tag="PKK95_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17407.1"
                     /translation="MAEAPGTTMAEGGKERGKGGDGEPEKAVVAAYRGFFPVVYGNDV
                     EVKQGGGAILIARDELEIEQGGGQCMIGLRKLEVEQGGCALMVAGRAQVDRGLIGILL
                     AGKAELGEGTRVLLTVPQAIAAGVAAGAIVALASLLRADRGRGRRPRPEIAGSTTPGI
                     "
     gene            complement(10667..11191)
                     /locus_tag="PKK95_04020"
     CDS             complement(10667..11191)
                     /locus_tag="PKK95_04020"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOC17408.1"
                     /translation="MGVDWGRVFAFDTPWLEIVVRGTAIYLGLFLLLRLVLKRQAGTV
                     GITDLLVIVLIADAAQNAMADDYRSVPDGLLLVAVIIFCSHALDWLGYRFPVLNRLVH
                     PPPLPLIRDGRLLRQNMRRELMTEEDLLSQLREQGVLDIKDVKVACMEGDGRVSVVPF
                     EKQQGSRGNERRVT"
     gene            11410..11955
                     /locus_tag="PKK95_04025"
     CDS             11410..11955
                     /locus_tag="PKK95_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17409.1"
                     /translation="MSVPRCALAVAAFLLSTAAVGAQTPNSSTLGAELAQLLEQRQLN
                     NVAAKDPEHPGFFVAASHIPGQQLLVLSARSTAPDYVEYVLGASKYDEVYATLNFASA
                     PDGKLLVQDMGCDGLRLEATESGTDVAYLDAAKTAIFNGDWKKQGVSEQEYRQTFEEV
                     EARYARALSLLVEQLKRPATE"
     gene            complement(11976..12113)
                     /locus_tag="PKK95_04030"
     CDS             complement(11976..12113)
                     /locus_tag="PKK95_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17410.1"
                     /translation="MNPQTNPRERESIAAKYFVAQLPTWILVSVASWAVPGAILVGAG
                     R"
     gene            12222..14045
                     /locus_tag="PKK95_04035"
     CDS             12222..14045
                     /locus_tag="PKK95_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOC17411.1"
                     /translation="MALPIEDYAIIGDTQTAALVGRDGSIDWLCLPRFDAPACFAALL
                     GTHHHGRWLIAPAGGGRAVSRRYLEDSLVLCQEFETAAGRVRVTDFMPPRDREPDLVR
                     LVEGVQGTVAVEMELVIRFDYGSIVPWVRSMDGRLFAIGGPDALSLWPGAATEGRDLT
                     THATFEVRAGEQVTFVLAWHPSHEELPNPAPDAAKALTDTLAWWRDWARRCTYHGPWR
                     EQVLRSLLTLKALTYAPTGGIVAAPTTSLPEKLGGVRNWDYRYCWIRDATFTLFALSI
                     GGYLEEAAAWRNWLLRAVAGDPADLQILYGPAGERRLPELELPWLPGYEESRPVRIGN
                     AASGQLQLDVYGEMMDVLDVARRAGLPPDRDAWALQTAMLRHLEKIWRAPDEGIWEVR
                     GPRRHFTHSKVMAWVAFDRAVTAVAEAGLDGPVDRWRAVRAEIHQEVCSRGFDVGLNA
                     FTQYYGSQIPDASLLMIPLVGFLPARDPRVAGTVALVERGLLRDGFVHRYPVEERQQE
                     VDGLPAGEGTFLPCSFWLADNYVLAGRYDEARALFERLIGVANDVGLLAEEYDVGARR
                     LVGNFPQAFSHVGLVNTARNLARAHGPAESRGEGRGAVPRA"
     gene            complement(14374..15558)
                     /locus_tag="PKK95_04040"
     CDS             complement(14374..15558)
                     /locus_tag="PKK95_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17412.1"
                     /translation="MAKTVNVALIGQGFMGRTHSNAYLKVAKFFTDLPAVPVMHTSFG
                     MKNENCNAFTSRWGWQHCSTDWEKTVRSEEIDLVDIVTPNYMHMEPALAALDAGKPVA
                     TEKPLAGTLADARTMVEAAKQAKVPTFVWYNYRRVPAVALAYQLVQEGRIGRVYHVRC
                     VYLQDWGGPDTPLAWRFQKKLAGSGAHGDLNAHIVDMARFVTGDDIVEVTGAIAETFI
                     KERSIASAGATGGIVQKTKSRAKGRVDVDDAVLFLARFRKGAVASFEATRLATGYKNR
                     NGFEIHGEKGALRFNFEDMNYLDFYDNTEDPKTASWRHIIVTSGGNHPYVGAWWPDAH
                     ILGYEHGFVNQAADIMHVLARRKPVVPVPDFADAYETQRVLEAALLSAKERAPIKISE
                     VK"
     gene            complement(15617..>16320)
                     /locus_tag="PKK95_04045"
     CDS             complement(15617..>16320)
                     /locus_tag="PKK95_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOC17413.1"
                     /translation="REEVVINDRWGSDSRHKHGGYWTTEYTPGMSGMEHPWEESRGMG
                     FSYGYNRAERLEHYRSGRELVMMLVDLVSRGGNLLLDIGPDGDGTIPVVMEERLVQIG
                     DWLKVNGEAIYGTRPWTATRQWSAGQVPATDYGKEYAAAYDVMALVAQPEAGKAAIEA
                     FFTAKGDDVYAILPRWPGRRFVVKQVSGVRSAALLGSASPLAFTAEGDGVSIELPEVP
                     EALRAQPAWVLKLGR"
BASE COUNT         2344 a         5823 c         5790 g         2363 t
ORIGIN      
        1 atgggcggcg gcgtggatcg agtcgggcgg gccgtcgctc gggaagccga ctcgctacga
       61 ggtggcagat ggccggtact gaggaggcga ggcggcggct cgccgcgggc ggcgtcatcc
      121 cggcacaccc gctggcgctc acccccgagc ggcggctgga cgagcggcgg caggtcgcgc
      181 tcacccgtta ctactgcgcg gccggggcgt cgggcatcgc cgtcggcgtg cacacgacgc
      241 agttcgcgat ccggcagccg agggtgggac tctacctgcc ggtgctgcga ctggcggccg
      301 acgccatcgg cgaacacgag cgggccaccg ggtcggccat cgcccgcgtc gcgggggtgt
      361 gcggccgcac gcggcaggcg gtcggcgagg cgggagccgc cgccggcctg ggctaccacg
      421 cggcactcct gagcctgggc ggctggaacg acgcctcgga cgacgagctc ctcgcgcact
      481 gccgcgaggt ggcgtcggtg atgccgttga tcgggttcta cctccagccg gcggtaggcg
      541 gacgcgcgct gtcggaggcg ttctggcgcc ggttcttcga gatccccgag gtcgtcgcgg
      601 tgaaggtggc gcccttcgac cggtaccgca cccaggaggt cactcgcgcg ctcgccgccg
      661 cggggcgcga gcgcgaggtg accctctaca cgggcaacga cgacgcgatt gtcgcggacc
      721 tcctgaccga cttccccggc tgcgccgggg gacagcgcct gtgcttccgc ggcgggctgc
      781 tcggccagtg ggccgtgtgg acccggcggg cggtggagct ggtggacgag atccgggccg
      841 agcgggcgcg gccgggcgag aagacgagcg agctgctggc gcgcggggcg gatctgacgg
      901 acgcaaacgg ggcgctgttc gacgcgcgcc acgccttcgc gggctgcctc cccgggatcc
      961 acgaggcgct gcgccgccag ggcctgctcc tcgggcgctg gtgcctggac gaacgcgagg
     1021 agctgtcggc gggccagatg gaagagatcg atcgggtgca cgcgcgctat cccggcctcc
     1081 gggacgacgc gttcgtcgag gcgcacctcg aggagtggct gcgatgaggc cgggggcaac
     1141 ggcggagggg atcggccttg acgggatcga acggatgcgt tcgaattccg gaggagtgaa
     1201 agccatgccc gggacggctc ggcaccgcct cctcgtggag gagcgccgcc gcaagatcct
     1261 cgaggccctc gaacaaagcg agcgcgtgac ggtccaggaa ctggtccggc ggttcggggt
     1321 gtcggcggtc acgatccgga aggacctcga cgcgctgacc gaggcgggcg cgatggtccg
     1381 gtctcacggc ggggcgctgc ggccgctcgg gcccaccgac atcgcgatcg gcgtcaagga
     1441 gatcctccac cacgaggaga aggtccgcgt cggcacggcg gcggcggccc tggtgcagaa
     1501 cgacgagacg atcatcctcg actcggggac gacgacgctc gaggtggcgc ggcagatcgc
     1561 cgcgcgcaag ttccggtcgc tgaccgtgat caccaacggc ctcaacatcg cggtggagct
     1621 ggcccgcctg cagaacatcc gcctgatcat gctcggcggt ctggttcgcc acgtgtcgca
     1681 ctcggtggtg gggccgcacg ccgagcagat ggtgcgcggg ctgaacgccg accggctctt
     1741 cctgggggtg gacgggctcg atctcgagat cggtctgatg acgcccgacg tgctcgaagc
     1801 gcagctgaac gggatgctga tccgggtcgc ccgcgaggtc aacgtggtca ccgactcgtc
     1861 gaagttcagc cggcgcagcc tgtcggtgat cgcgaggctc gacgccgtcc accgcatcat
     1921 caccgacgac gggatcgacc ccgccttcct ggcggcgctg cgcgcgagga gcatcgaggt
     1981 gctgacgacc tgaccggcgg cgtcagtggg acaggcgtct tgcctgttgg agggcaactc
     2041 ctgtaacaac tccagtggga caggcgtctc gcctgcctgt ctctgcgcgc tgggctgtgg
     2101 tcatcgggct gctaccggcg ggggaactgg tcggggttgt cggtaaagag ggcgtcgacg
     2161 ccgaaggtgt agaggaactg ctgcatctcc tcacgcacgg tccggccggc cggctggccg
     2221 gcggagcgga acgtgtaggg cgtgacgctg aggccggcct cgtgcgccca gacgacgacc
     2281 gcggggcgag ccgagacgat gcccttggcc gggccgatgc cgtccgcgaa cgccttgatc
     2341 tccttcatcc gctggcgcgt cagccaggtt tccgcgtcgt tctcgccaac caggaagagc
     2401 agcggcagcc gcactcccat cgcccgcatc ttcttcaggc tctcggcgct gaacgactgg
     2461 atgaagacgg gcgtctgcgg atcggcgccg ggctcggcca ggccgtgacg ccccagcgcg
     2521 tcgatcacga gccgctccat gtcgaagccg agacggccgt agacctcggg cgccttggtc
     2581 tccgggtaca ggcctccgcg tcccttgacc aagccgatcg cctcgtcgaa ggtcagcacc
     2641 ttctcgccct tgaacttcgg agcgaaccag gagccggcgt cgagcctctt gatctcgtcg
     2701 agcgtgaagt cggagacgtg ccagccggtg accggcttcc cctcacgctc caccgttcgc
     2761 gagcggccgg gaaacagatc gcggacgttc gtcgtccgct cgagcgtcag atcgtggagg
     2821 cagaccagcc ggccgtcttt cgtgatctgc aggtcctgct cgacgtagtc ggcgccctgc
     2881 tcgatcgcaa gcctgtaggc cgccgcggtg tgctcgggcg cgtaggccga cgccccgcgg
     2941 tgcgcgatcg ccgtcttccc ccggtactgc gcggcgccat taccgaacgc cagcacgccc
     3001 gccaccagca tgatgactat cctgggatgc gacatgacgc cccatgatag ttgaagaagc
     3061 tgacagcagc ccacaggcgg gacgcctgtg ccactggctg tcagctgcgc acaggcggca
     3121 cgcccgtgct actggctgcc ccaagcaacc aagtcagccg atcgtaaacc gcctcggcca
     3181 ggcggggatc ggaaccgtgc tcctcgacgg tctggacgat cgcgcttcca accaccgcgg
     3241 cgtcgctcca gcgtgcggcc tcgcgcacgt gctcgggctg cgagatgccg aagccgagcg
     3301 cgatgggcag gctggtctcg gcccggatgc gcgtcaccag cgcccgcgca ccgtccgaca
     3361 gtctctcgcg cgccccggtg acgccgagcc gggagacggc gtagagaaaa ccgccgccga
     3421 gcgcggcggc gcggcggatc cgctccgggc cgctcgtggg cgcgaccagc aagatggtgt
     3481 cgaggcccga gccggcggcc gcgtcgcgga acggcccggc ctcctcgagc ggcaggtcca
     3541 cgacgagcac gccgtccacg ccggcctgcg ccgcctcccc ggcgaatcgt tcgacgccga
     3601 ggcgcaggac cgggttcagg taggtgaaga gcacgatggg cgcgcgggcc tggcggcgga
     3661 cgcgtgccgc cagcccgatc gcgcgcagga gcgtcgtgcc gccgcgcagc gcgcgattgg
     3721 acgcgcgctg gatgacgggg ccgtcggcga gcgggtccga gaagggcacg ccgatctcca
     3781 gcaccgaggc gccggcggca tcgagcgcga ggaacacggc ttccgaacgc ggaaggtcgg
     3841 gatcgccggc ggtggcgaag gccaccagtc ccggctgccg ggccgctcgc agcgtggaga
     3901 aagtcgaatc aagtcgcgac attcgagagg tccttgtccc cgcgcccgga gaggttgacg
     3961 aggatggtgc gcccgatccc cagttcgggc gccaggcggc acgcggacgc gagggcgtgg
     4021 gccgactcga gggcgggcag gatgccctcc agcctggcga gggcgtggaa cgcggcggcc
     4081 gcggcgtggt cgtctgcagt ggtgtactcg cagcggcccg acgcccgcag ccacgcgtgc
     4141 tcgggcccga cggccgggta atccagcccc gccgaaatcg agtgcgtgga gaggatgttg
     4201 ccggcctcgt cctggaggac gaaggtgcgc gtgccgtgga ggacgccggc gctgcccgcc
     4261 gcgaagcggg ctgcgtgccg gcacggcgca aggctctcgc cgccggcctc cacgccgacg
     4321 agcctgacgg aggcatcgtc gaggaacgcg tcgaagatgc cgatggcgtt gctcccgccg
     4381 ccgacgcagg cgacgaccgc gtcgggcagt ccgccggtcg cgccggcgag ctgccgccgc
     4441 gcctcgcggc cgatgaccga ctggaattcg cggaccatga gcgggtaggg gtgcgggccg
     4501 agcgccgacc cgagcaggta gtgggtgtcg tgcggccggg cgacccagtc gcgcatcgcc
     4561 tcgttgacgg cgtccttgag agtgcggctg cccgagccga cctcgatcac cttcgcgccg
     4621 agcagccgca tgcgcgcgac gttgggcgcc tggcgcgcga cgtcgtcggt tcccatgtag
     4681 acctcgcagc caaggccgac gagggcgcac gcggcggccg cggccacccc gtgctgcccc
     4741 gccccggtct cggccaccac gcgcgacttc cccatgcggc gggcgaggag cgcctggccg
     4801 agcgcgttgt tgatcttgtg cgcgcccgtg tgcgcgaggt cctcccgctt gaggaagatg
     4861 cgcgcgccgc cgcaggccgc cgcgaggcgc ctcgcctcgt ggagggcggt gggccggccg
     4921 acgtagtcgc gcagcaggcc gaggtactcg cgcgtgaacg ccgggtcgcg gcgcgcctcg
     4981 tcgtaggcgc gggtcagcgc ctcgagcggc tccacgagcg tctcgggcac gaaccgcccg
     5041 ccgtactcgc cgaagtagcc gcctggatcg gggtcgcgaa gaccccactc gatcgcgtga
     5101 gccgtcatgc ccgtccccct gcctggcgca cggccgccac gaagcggcgc atgcgctcgg
     5161 cgtccttgat gccgggcgcc gcctcgacgc ccgacgagac atccaccccg tgcggcgcaa
     5221 cccgggcgat cgcctcagcc acgttctccg gcgacaggcc gcccgacagg atgacccgcc
     5281 gccggcgcgc cacgcgtgcg gccacttccc agtcggccct ggtgcccgta ccgcccttcg
     5341 actccggcgc gtcggcgtcg agcaggacgg tcgcctccgc gtacgcgtca acgagcgagg
     5401 gatcgaatgc cggccccacg ccgaacgcct tgatgaccgg ccgcggcatc tgccggcacg
     5461 cctcgggcgt ctcggcgccg tgcagctgca cgaggtgcag ccccacccgc gaggctacca
     5521 gccgaacccg ttccaccgcc tcgtccacga agaccccgac ggcagccacg ccggctggca
     5581 gcgcgcccgc gatgcggcgc gcgcgctcca cgtccacgga ccgccggctg tgcggccaga
     5641 agacgaggcc gatcgcgtcg gcgccgagcg aagccgccag cctcgcatcc tcctcccgcg
     5701 tcacaccaca aatcttcagc cacatacacc tgcctcgacc ggacgaccga tcaaccacaa
     5761 agatcacaaa ggggtgtggc cggtgcggtc gccagcgcaa tcggatccct ccgttgttct
     5821 tcgttcttcg tgtcctttgt gtcctttgtg gttcaagctc gttcgtcctt ggttccgatc
     5881 aactcctgga gcgcggctcc cgggtcggcg gcggtcatca ggcgctcgcc aacgagcagc
     5941 gcgtggtagc cgcacccgtg cagtcgcacg gcgtcggcgc gggtcctgat gccgctctcc
     6001 gcgacggccg tgacgccggg tggcaggtgt ccggccaggc ggacgctcgt ctcgaggtcc
     6061 acccccatcg tccgcaggtt gcggttgttg acgccgacca gcgtggcgcc cgcctccacg
     6121 gcccgcacca gctcgccttc gtcgtgcact tcgacgagtg ccgccaggcc gagcgagcgc
     6181 gccaggccgg cgagcgggcc gagctcggcg ccgaggacgg cggcaatcag cagcaccgcg
     6241 tcggcaccgg ccgcgcgcgc ctcgacgacc tggtagccgt cgagcacgaa atccttcctc
     6301 agcactggga gagtcgttgc cgcgcggacg gcttcgaggt cgccgatcga gccgccgaag
     6361 tgcggcgtgt ccgtgagcac cgagagggcc gccgctccgg cgcgctcgta ggcgcgcgca
     6421 atggccgccg ggtcgtacgc ggcccgtagc aaccccttcg agggcgaccg gcgcttgcac
     6481 tccgcgatga tggccgggcc cgggcccgag cgaagggcgg cggcgaacgc ctcgcccctc
     6541 ggctcgcgcc ccagggccgc acgctccatc gaggcggccg gcacgcgccg ccggcgctcc
     6601 tccaacgacc gcctcgccgc caggacaatg tcgtcgagcg acgcggctgt cctcgtcccg
     6661 cccatcatgc gtgctcctcg catccgtctt cgtgcgagag ccgcgccatc agcgcgagcg
     6721 tgcgcttcgc gtcgccgcca ccgatcgccc ggcgcgccag ctccgcccct tcctcgaggc
     6781 tggttgcgcg ccctgcgaca aacagcgccg cacccgcgtt catggcgacg acgtcgctcg
     6841 cgggccccgg caccccgtcg aggacgagcc gcgcgatccg gacgttctcg gccacgccgc
     6901 cgcctgcgag cgcttcggcc gaagcgggcc gcaggccgaa gtcggacggg tgcacgtaga
     6961 agctgttcac cgcgccgtcg cggcactccg acaccttcga gtacccggcc gtggacagct
     7021 cgtcgaggcc gccctcgccg tgcaccaccc acgcgcggcg gcacccaagg gcggcgagcg
     7081 cgcgggcgac caggccggtc agttcgggcc gcgagacgcc gagcagctgg tgcgacggcc
     7141 gcgccgggtt ggtgagcggg cccagcaagt tgaacgccgt ccgcatcttc agctcgcggc
     7201 ggaccggcgc cgcgtgccgc atcgacgggt ggaacgaggg ggcgaagagg aaggcgatgc
     7261 cggccgcctc gaggcagcgc gccacgatgc cgggcggtgc cgagagactc acgcccaggc
     7321 cctcgaagag gtcggcgctg ccgcaggcgc tcgaaacccc gcggttgccg tgcttggcca
     7381 cgcggacgcc gcacgcggcc acgacgatgg cggcgagcga cgagatgttg aaggtgcccg
     7441 agcggtcgcc cccggtcccg cacagatcga cgagcgggcc cgatgccgcg ccaggcgcgg
     7501 aacgcagcgg caggggcacg gcgtgctcgc gcatcgcgcg cgccagcccg gcgatctcgg
     7561 cggcgcgcgg ccccttcatc tcgaggccga cgagcagccc cgagatctcg gcgggcgtcg
     7621 cctgcccctc catgatggcg ttcatcgacg ccacggcctg ctcggtggtc aggtcgtggc
     7681 gccggccgag ccgctcgagg acctccgcga acatacctgc ctcctccctg ttcacatcgc
     7741 gaggaagttg cgcagcaggt ggacgcccgc tgccgtaagg attgactcgg ggtggaactg
     7801 caccccgtgg actggcagcg tgcggtggcg gaggcccatg atggcgccgt ccccggatgc
     7861 gcgcgcggtg acctcgagtt ctgccggcag cccggcgtcg gccacgacga gcgaatggta
     7921 gcggccggcg gcgaacggcc cgggcaggcc ggcgaacacg ccggccccgt cgtgctcgac
     7981 gagcgaggcc ttgccgtgca ccggccgcga cgcgcgcacc acccggccgc cgaacgcgag
     8041 gccgatggcc tggtgcccga ggcagacccc gagcaccggg acggtggagc cggccccccg
     8101 cacgagatcg agcgtggcgc ccgcgctctc gggccgtccc gggccgggcg agacgaccac
     8161 gtgagagggg cggagcgcga gcatcccggc ggcgtcgagc gcgtcgttgc gcagcaccgt
     8221 cacgcgggcc ccgagcgccc cgaggcactg gaccaggttg taggtgaacg aatcgtagtt
     8281 gtcgacgacc aggatcatcg gctgcctcct accactcgct ggacgcggcg gcctcgagcg
     8341 cctgcagcag cgcccgcgct tttgcgcacg actcctcgta ttccgcggcg ggattggagt
     8401 cggccacgat gccggcgccg gcctgcaccg aagcacggcc cggctccagc accaccgtcc
     8461 ggatcgcgat gcagaagtcg aggttcccgg cgaagtccac gtaccccacg ccgcccgcgt
     8521 agacgccgcg gcgcgacggc tcgaccgcct cgatgatctg catcgcgcgg atcctcggcg
     8581 cgcccgacac ggtgccggcc gggaagcagc tggcaagggc gtcgagccga tcgcgaccaa
     8641 ggtcgagccg cccctcgacc accgacacca ggtgcatcac gtgcgagtag cgctcgatcc
     8701 ccatcagctg cgccacccgc acggagccgt acgtgcagac gcgcccgaga tcgttccgcc
     8761 cgaggtccac cagcatcacg tgctcggcac gttccttctc gtcggaggcc agctccgcgg
     8821 ccaggcgcgc gtcctcctcc tcgtcgcggc cgcgcgggcg ggtgccggca atcgggtgcg
     8881 tctcgacgcg gtcgccctcg acgcgcacca gcatctcggg cgagcctccg gcaatcgccg
     8941 tccgcccggc gcgaatgaag aacatgtacg gcgaggggtt gatgtggcgg agcgctcggt
     9001 agacccggaa gggatcgacg gaggtctcca cgtcgaaccg ctgcgagagg acggcctggt
     9061 agatgtcgcc ctccgcgatg cgttcctgca cggcgcggac cccgcgctcg aactcctcgc
     9121 gcgagacgtt cggccgcggc accaggggtg atgccgcggg cggctcgggc gtcgagacct
     9181 cgcgtgccag ctcccgctcg aggaagccga tccgggcgca cgcgaagcgg tacagcgcct
     9241 cgaggtcggc gccgggcgtt acccgggcgt tcgcgatcac gaggatgcgg tggcggacgt
     9301 ggtcgaacgc caggatcgtg tcgaacacca tgaagacggc cgggacggga caatcgccgg
     9361 cccgccggac ggccggctcg aaccacccgg ccgcgtcgta gccgaggaag ccgaccgcgc
     9421 cgccagtgaa gcgcgggagc cccggcaccg agggggcgcg gtactccccc atcacccgcc
     9481 tcaactcctc cacgatggtg ccgccggcca ccgtcgtctt ccccgcctgc tccaccaggg
     9541 tgcggtggga atcggcgcga aggacgagca gcgggtcctt gcccaggaac gagtaccggg
     9601 ccacctgctc gccgccttcc acgctctcga gcaggaacga gtagtcggag tactcggcaa
     9661 ccttgagaaa ggccgagacc ggggtcagca ggtcggccat cagctccttg cagaccggca
     9721 cgaacgagcc ctcgcgcgcg agacgggcga actcctcgaa ggtggtcatg gtcatcccgg
     9781 tcgtctcccg atctgccgta caaacaaaaa gccccttcag cggtttccgc tgaagaggct
     9841 ctggagaacg ctctgctcga gatgtgtcta cacgggcccc ctcagtggat ggtgttccgc
     9901 caccaccacg aagaggacga cgcgccgcgg gcggccacac tcccgcgcgg atcgacgccg
     9961 aggccggcgg gcagggccgg caggcacggc gacgggcagc ggcggaagca gttcgtcaca
    10021 atcaacctga gaggtcgaac ggtagcagcg actgggccgc gttgtcaacc agcctgcccg
    10081 ccgcctcgcg ggagtgcggc tggcggggcg tctttcggcg gccgctaaat gcccggcgtg
    10141 gtgcttccgg caatctccgg ccgtgggcgg cgtccgcgtc cgcgatctgc ccgcagcagg
    10201 ctggcaagcg ccacgatcgc tccggccgca acgccggccg cgatcgcctg cggcacggtc
    10261 agaagcacgc gggtgccctc ccccagctcg gccttcccgg ccagcaggat cccgatcagc
    10321 ccccggtcga cctgggcccg gccggcaacc atcagggcgc agccgccttg ctcgacctcg
    10381 agctttcgga ggccgatcat gcactgcccg ccgccctgct cgatctccag ctcgtccctg
    10441 gcgatgagga tggcgccgcc gccctgcttg acctcaacgt cgttgccgta gaccaccggg
    10501 aagaagcccc ggtacgccgc gaccacggcc ttctccggct cgccgtcgcc gcctttcccc
    10561 cgctccttcc cgccttccgc catcgtcgtg cctggtgctt cggccatgag gacccctctc
    10621 tccgctccca atcgcctggc tgccggttgt gacctcgccg ccacggtcat gtcacgcgcc
    10681 gctcgttgcc ccgagagccc tgctgctttt cgaagggcac cacgctcacc cgtccatcac
    10741 cctccatgca cgccaccttg acatccttga tgtcgagcac gccctgctcg cggagctgcg
    10801 acagcagatc ttcctctgtc atcagctcac gccgcatgtt ctggcggagc agccggccgt
    10861 cccggatgag gggcagcggc ggaggatgca cgaggcggtt gagcaccggg aacctgtagc
    10921 cgagccagtc gagcgcatgg ctgcagaaga tgatcacggc caccaggagc agcccgtcgg
    10981 ggaccgaccg gtagtcgtcc gccatcgcgt tctgagccgc gtcggctatc aggacgatga
    11041 cgaggagatc ggtgatgccg accgtcccgg cctgccgctt gagaacgagg cgcaggagca
    11101 ggaacaggcc gaggtagatc gcggtgcctc gaacaacgat ctcgagccac ggcgtatcga
    11161 acgcgaagac ccgtccccag tcgacgccca ttcaggcccg gcccggttgc ccgtgtgaca
    11221 cgccaaggtc catgcgaacg tggtgccggg gccatcccgc cggctccgca gggcgctgtc
    11281 gcccggaagt cccgttggcg ggcgggggga cctcgggcgg gcaggggaat gccgggaccg
    11341 ggattttgat ccaaactgta ggtgtgaacc cggctgtagg cctgccggga gaggagaaag
    11401 gagcttgcca tgtccgtgcc gcgttgcgct ctggccgtcg cggctttcct gctttcaacc
    11461 gctgcggtag gagcgcagac accgaactca tccacgctgg gggccgagct ggcccagctg
    11521 ctcgaacaac gccagctgaa caacgtcgcc gccaaggacc cggagcaccc cggatttttc
    11581 gtcgcggcat cacacatccc gggacagcaa ctgctggtct tgtcggcccg gtcgaccgcg
    11641 cccgactacg ttgagtacgt cctgggcgcg tccaagtacg acgaggtcta cgccaccctc
    11701 aacttcgcct ccgcgcccga cgggaagctg ctcgttcagg acatggggtg tgacggcctc
    11761 cgtttagagg caaccgagag cgggaccgac gtcgcctacc tggacgcggc gaagacggcc
    11821 atcttcaatg gcgactggaa gaagcagggc gtcagcgagc aggagtaccg ccagacgttc
    11881 gaggaggtgg aggccaggta cgcgcgggcg ctgtccttgc tcgtggagca gctgaagcgt
    11941 cccgcaaccg agtgaccgag tgggcgggcc gttcgtcagc ggcccgcccc cacgaggatg
    12001 gcccccggga cggcccagga ggcgaccgac accaggatcc atgtcgggag ctgcgcgacg
    12061 aagtacttcg cggcaatcga ttcgcgctcg cgcgggttcg tctgcgggtt caccacgccg
    12121 gccctgatgc gagcgtcggg ccagcgtgcc ggatcgtgcc gaccgccgcc ggcctccgtg
    12181 ccgggaactc ctcttgctac gctcgccgct ggtggcggcc gatggctctt cccatcgagg
    12241 attacgcgat catcggggat acccaaacgg ccgcgctggt cggccgcgac ggctcgatcg
    12301 actggctctg cctgccccgc ttcgacgcgc cggcctgctt cgcggccctg ctgggcactc
    12361 accaccacgg gcgctggctc atcgctccgg cgggcggagg ccgtgccgtc tcgcgccggt
    12421 acctcgagga ctcgctcgtg ctctgccagg agtttgagac cgcggcagga cgggtgcgcg
    12481 tcaccgactt catgccgccg cgcgaccgcg aacccgacct ggtccgcctg gtcgagggcg
    12541 tgcaggggac cgtggccgtc gagatggagc tggtgatccg gttcgactac ggctcgatcg
    12601 tcccctgggt ccgctcgatg gatgggaggc tgttcgccat cggcggcccg gacgcgctga
    12661 gcctttggcc cggtgccgcg accgaggggc gcgacctcac cacgcacgcg accttcgagg
    12721 tccgggcggg tgagcaggtg acgttcgtcc tggcctggca cccgtcgcac gaggagctcc
    12781 caaacccggc gcccgacgcc gcgaaggccc tcaccgacac gctggcctgg tggcgcgact
    12841 gggcgcgccg ctgcacctac cacggaccct ggcgggagca ggtgctccgc tcgctcctca
    12901 cgctcaaggc gctgacctac gcgcccacgg gggggattgt cgccgcgcca acgacctcgc
    12961 ttcccgagaa gctaggcggg gtgcgcaact gggactaccg ctactgctgg attcgcgatg
    13021 cgaccttcac cctctttgcc ctcagcatcg gcggctatct cgaggaagcg gcggcctggc
    13081 gcaactggct gctgagggcc gtcgcgggcg acccggcgga tctgcagatc ctctacggtc
    13141 ctgccggtga gcggcggctc ccggagttgg agctcccgtg gctgcccggc tacgaggagt
    13201 cgcggccggt ccggatcggg aacgcggctt ccgggcagct gcagctcgac gtctacggcg
    13261 agatgatgga cgtgctggac gtggcgcgcc gggcgggctt gccgcccgac cgcgacgcgt
    13321 gggccctgca gacggcgatg ctgcgccacc tcgagaagat ctggcgtgcg cccgacgagg
    13381 gcatctggga ggtgcgcggc ccgcggcgcc acttcacgca ctcgaaggtg atggcgtggg
    13441 tcgcgttcga ccgggcggtg acggcggtgg cggaagcggg cctcgacggc ccggtggacc
    13501 gctggcgcgc ggtgcgcgcc gagatccacc aggaggtgtg ctcgcggggg ttcgacgtcg
    13561 ggttgaacgc cttcacccag tactacgggt cgcaaatccc ggacgcgagc ctgctgatga
    13621 ttccgctcgt cggcttcctc cctgcgcgag atccccgcgt cgccggcacc gtcgccttgg
    13681 tcgagcgagg cctgctgcgc gacgggttcg tccaccgcta cccggtcgag gagcggcagc
    13741 aggaggtgga cggcctgccg gctggcgagg ggacgttcct gccctgctcg ttctggctgg
    13801 ccgacaacta cgtgctggcc ggccgctacg acgaggcgcg cgccctcttc gaacgcctga
    13861 tcggcgtcgc caacgacgtg gggctgcttg cggaggagta cgacgtcggc gcccgccggc
    13921 tggtgggcaa cttcccccag gcgttctccc acgtggggct ggtgaacacc gcgaggaacc
    13981 tggcgcgggc gcacggcccg gccgagagcc gcggcgaagg ccgcggggcc gtaccaagag
    14041 cgtgaaagac gtcacacgtg gggccaagat cgtgtgacgc tggatgcgtg tccacgttca
    14101 acccgcgttt actctacgcg ctttcctgat gatcttttgc gtctcggtgt ctctgtgtct
    14161 ctgtggttca gtcaacgtga tctcgaatgc agcgtgacag cgcctttgaa gttgctgatt
    14221 cgcagatctc tgcgtacgcc tccgcgcctc cgcgtctccg cgtctccgcg ttgaacgtgg
    14281 tcgcggccgt gctcctgtac gcgaacgccc gcggccgttc gaccgcgggc gtcgtgtgcg
    14341 cgcctccgac ggggcgacca caagggtcgc cccctacttc acctccgaga tcttgatcgg
    14401 cgcgcgctcc ttcgcgctga gcagcgccgc ctcgagcacg cgctgcgtct cgtaggcgtc
    14461 ggcgaagtcg ggcaccggga cgacgggctt ccggcgggcg aggacgtgca tgatgtcggc
    14521 cgcctggttc acgaagccgt gctcgtatcc gaggatgtgc gcgtcgggcc accacgctcc
    14581 gacgtacgga tgattccctc ccgaggtgac gatgatgtgg cgccacgagg cggtcttggg
    14641 gtcctcggtg ttgtcgtaga agtcgaggta gttcatgtcc tcgaagttga accgcagggc
    14701 ccccttctcg ccgtggatct cgaagccgtt gcggttcttg tagccggtcg ccaggcgcgt
    14761 cgcctcgaag ctggccaccg cccccttccg gaacctcgcc aggaacagca ccgcgtcgtc
    14821 cacgtccacg cgtcccttgg cccggctctt cgtcttctgg acgatgccgc cggtggcccc
    14881 ggccgaggcg atcgagcgct ccttgatgaa ggtctcggcg atcgcgccgg tcacctcgac
    14941 gatgtcgtcg ccggtgacga agcgggccat gtccacgatg tgggcgttga ggtcgccgtg
    15001 ggcgccgcta cccgccagct tcttctggaa gcgccaggcg agcggcgtgt cggggccgcc
    15061 ccagtcctgg aggtagacgc agcgcacgtg gtagacgcgg ccgatccggc cctcttggac
    15121 cagctggtac gcgagtgcca ccgctggaac ccggcggtag ttgtaccaga cgaaggtggg
    15181 caccttcgcc tgcttggctg cctcgaccat ggtccgggcg tcggcaagcg taccggccag
    15241 cggcttctcg gtggcgaccg gcttgccggc gtcgagcgcc gccagcgccg gctccatgtg
    15301 catgtagttg ggcgtgacga tgtcgaccag gtcgatctcc tcgctgcgaa ccgtcttctc
    15361 ccagtcggtg ctgcagtgct gccagcccca gcgcgaggtg aaggcgttgc agttctcgtt
    15421 cttcatcccg aacgacgtgt gcatgaccgg gacggccgga aggtcggtga agaacttggc
    15481 aaccttcagg taggcgttgc tgtgggtgcg ccccatgaag ccctgtccga tcaaggccac
    15541 gttcacggtc tttgccatcg gtcctgcctc ctttgactgt gcgattcgcc gcgaggacgg
    15601 cgtcgattat cgtcgctcac cggccgagct tcaacaccca ggcgggctgc gcgcgcagcg
    15661 cctcgggcac ctcgggcaac tcgatcgaca cgccgtcgcc ctcggcggtg aacgcgagcg
    15721 gcgaagccga tcccagcagc gccgccgacc ggacgccgct cacctgcttg acgacgaaac
    15781 gccgtcccgg ccagcgcggg aggatcgcgt agacgtcgtc gcccttggcg gtgaagaacg
    15841 cttcgatggc ggccttcccc gcctcgggct gcgcgacgag cgccatgacg tcgtaggccg
    15901 ccgcgtactc cttgccgtag tcggtggccg gcacctggcc ggcgctccac tgccgcgtgg
    15961 cggtccacgg gcgcgtgccg tagatcgcct cgccgttcac cttcagccag tcgccgatct
    16021 ggacgaggcg ctcctccatg accaccggga tggtgccgtc gccgtccggg ccgatgtcga
    16081 gcagcaggtt gccgccgcgg ctcaccaggt cgaccagcat catcaccagc tcgcggccgc
    16141 tgcggtagtg ctcgaggcgc tcggcgcggt tgtagccgta gctgaagccc atgccgcggc
    16201 tctcctccca gggatgctcc atgccgctca tgccgggcgt gtactcggtg gtccagtagc
    16261 cgccgtgctt gtgccgcgag tcgctgcccc accggtcgtt gatcaccacc tcctcgcgga
//
LOCUS       DAOPQX010000044        16153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4296_length_16153_cov_4.602684, whole genome shotgun sequence.
ACCESSION   DAOPQX010000044 DAOPQX010000000
VERSION     DAOPQX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 16153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16153
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_16153_cov_4.602684"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKK95_04050"
     CDS             <1..255
                     /locus_tag="PKK95_04050"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17414.1"
                     /translation="LLGPREALEEALFMGLRLCDGVSCEALGARYGVDIWAVYGERLE
                     PAIDAGLLIRQGECLRLTRPGMLMANEVMAAFLRTDSTVE"
     gene            294..785
                     /locus_tag="PKK95_04055"
     CDS             294..785
                     /locus_tag="PKK95_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17415.1"
                     /translation="MRLTWFAIVTCRRPMLAGLVLALVLAFGSPTLTFAQEEQGAPQK
                     PTVAFQNDAGLMILYVKADKTADFEELAARLKEGLTKIEAPEVKQQAESWKLFKGPVG
                     NGTAVYVMLADPVVKNVEYWFLSLLYKAFPADAQALYQKWTDAKAANPPAVFDLTLVT
                     KMQ"
     gene            complement(867..1352)
                     /locus_tag="PKK95_04060"
     CDS             complement(867..1352)
                     /locus_tag="PKK95_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17416.1"
                     /translation="MARRGMVAALVVFAWLGAAAPARADITAFLGLSTTPGSRQARGL
                     AIGGGVLALGFEFEYSDIAEDAGDLEPGLRVGSVNGLLQTPIALGGAQLYVTAGAGVY
                     RERLGGSSETSLAVNLGGGAKIRLLGPLRLRLDYRLFSLRGDPLHDRYHRLYAGANLG
                     F"
     gene            complement(1391..2236)
                     /locus_tag="PKK95_04065"
     CDS             complement(1391..2236)
                     /locus_tag="PKK95_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC17417.1"
                     /translation="MPCPICDDTRWRVVEREGRREAVRCDCWREAVAARRLAEARIPR
                     RYQHCTLESFVTYPNEKLQNALSRARRFADAFPVVDKGLFLIGPPGVGKSHLAVAVLR
                     QVILARGARGLFYDVRELLRLIRSTYDPSVRANESEVLRPVMDADLLVLDDIGSEKSS
                     EWVEETLNLIVSTRYNDRRLTVFTSNYEEKEDAGDPDSLLARVGFRMHSRLYEMCEFL
                     EYDGADYRHRPPNAGPEDLLAMWKVQQGKRAVLPRKAGTQVRAQLRKPDPRADVAWPG
                     GRAGT"
     gene            complement(2500..2958)
                     /locus_tag="PKK95_04070"
     CDS             complement(2500..2958)
                     /locus_tag="PKK95_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC17418.1"
                     /translation="MVTRRGGKAYYMISAVAQKYSIHPQTLRLYEREGLLKPSRTEGN
                     TRLYSEEDLEQLETILSLTRDLGVNLAGVEIILNMRRKIEQMQHEVNEFMAYVKGELA
                     RGLDDWEQRLHTALVRSAPSDLVRSTPPPPDQQAPVAEKAGGRPRASAKS"
     gene            complement(2968..4086)
                     /locus_tag="PKK95_04075"
     CDS             complement(2968..4086)
                     /locus_tag="PKK95_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOC17419.1"
                     /translation="MDLYEILGVRRGAGGGEIRRAYRRLARRYHPDVNPGDREAQARY
                     ALITRAFETLNDPERRRAYDAGVPLEPAEPAQSYGFEGFDFSVQASVGAEASTFGELF
                     ADVLTRTLGGGEAGPQRGAHLHAAFSVSLAEVLTGTERQVPLTRHVACRACLGRGVVH
                     APAGDCPACRGTGRVRSSRGHMVFARPCERCGGSGQLTAMTCRHCGGQGVEVRGETVR
                     VAVPPGIADGAELRVAGGGHAGRRGGEPGDLFVRVHVEPHPLFRREGPDLHLVLPVAI
                     HEAALGARIEVPTLEGAVTVRVPPGTQSGQRLRLRERGLPMPRQGSRGDLLVEVKLVL
                     PSLLDERSKELLREFARIHHEDVRREWRAAEGAGRRLD"
     gene            complement(4136..6055)
                     /gene="dnaK"
                     /locus_tag="PKK95_04080"
     CDS             complement(4136..6055)
                     /gene="dnaK"
                     /locus_tag="PKK95_04080"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOC17420.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPSVITNPEGGRLTPSVVAFTK
                     TGERPVGQVAKRQAVTNPENTIFSIKRFMGRRYDEVTEEMTMVPYKVVAGQGGDVRVV
                     AMGKEYSPPEISAMILQKLKQAAEAYLGQTVTAAVITVPAYFNDAQRQATKDAGQIAG
                     LEVKRIVNEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGEGVVEVKSTNGDT
                     HLGGDNLDQRIIDWIITEFRKSEGIDLGKDRMALQRLKEAAEKAKMELSTVTETDINL
                     PFITADQTGPKHLTMRLTRSKFEQLVEDLIQKTVGPVRRALADAGLDASKIDEVVLVG
                     GSTRIPRVQQVVKELFGKEPHRGVNPDEVVAVGAAIQAGVLAGEVKDLLLLDVTPLSL
                     GIETLGGVMTKLIPRNTTIPTRKSEMFSTASDNQTQVEVHVMQGEREFARDNRTLGRF
                     NLVGIPPAPRGVPQIEVTFDIDANGIVNVSAKDMATNKEQKITITASSGLSKDEVDRM
                     MREAESHADEDKRRREVTDARNQADGLIYQIEKTLNENRERVAAGDASRIESSIAALR
                     KAAEGDDVSAIRKAMDDLQKASHAMAEALYRQSQPGGPGAQPGEEAGAGASAGGEGRT
                     GSDDVIDAEVVDDKK"
     gene            6322..8640
                     /locus_tag="PKK95_04085"
     CDS             6322..8640
                     /locus_tag="PKK95_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOC17421.1"
                     /translation="MAHVVLRVARHAGIVALFVLAAVLGVVTGVFFAYAGDLPRISAL
                     DDYAPNTITRVLAADGQVVAEFATERRVVVGYDDISARLRQAIIAAEDAGFYRHVGVS
                     MTRVLITAVEDVIKRRMAGASTLTMQLARGLFLTDEKTWERKIKEALLALQIEKRYTK
                     REIFTFYANQIYLGHGAYGVEAASRMYFGKRSKDLTLEEAALIAGIIQLPQRQSPFVD
                     VRRATGRRNYVLRQMAEEKYITREEAEAASRQPVVARGQPRADESIAPFFVEEVRKYL
                     ERKYGARQLYEKGLSVETSLDPQLQLAANQALDEGLRALDRRRGFRKPARNLVSEGVD
                     LARYTDDRWSRPRAVGQVLPAVVIAANPRGARTARGGATAPGGALAPGAVRVRIGEQT
                     ADLDRQAIAWTRRANASDFLRVGDLVEVRVRAIDPATGALSVVLDQAPIVDGALLAMD
                     NRTGQVVAMVGGLSFTRSKFNRATQASRQLGSVFKPFVYTVAIDRGFTPASVLLDEPV
                     AYDVGPGQPPYTPRNYDGTFEGPITLRRAVEQSRNVPAVRTIDQVGPGQVVDYVRRFG
                     FEASFPPVLSLALGAGEATLLETVAAYAVFPNQGIRMQPYQVLRILDRDGNVLEENRP
                     QPREALRADTAYVMTNLLRGVVQRGTAASAASLQWPLGGKTGTTNDFTDAWFIGFDPN
                     ITVGVWVGYDDKKPLGPAETGAQAALPIWKAFMKAYIDARGDRQNPPAFEAPGNIVFL
                     NVDRATGHPAEADAPNVISEAFIAGTQPGGWQ"
     gene            complement(8673..9746)
                     /locus_tag="PKK95_04090"
     CDS             complement(8673..9746)
                     /locus_tag="PKK95_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOC17422.1"
                     /translation="MSIEVRGVTKRFGQFVAVDRVDLLVQTGELMALLGPSGSGKTTL
                     LRVIAGLEIPDAGSVLFDGEDSTNRRAADRRVGFVFQHYALFRHMTVFENIAFGLRVR
                     PRETRPARPEIERRVQRLLELVQLEAMAHRLPAQLSGGQRQRVALARALAVEPRVLLL
                     DEPFGALDAKVRKELRRWLRRLHDELHVTSIFVTHDQEEALEAADRVVVMNQGRIEQV
                     GTPAEVYQHPVSPFVVDFLGDVNLFRFRIEHGKAYIGGLAFEVPHREDVREARLATLY
                     IRPHQLEIRLEEGGAQQFRARVRHVNPAGPLVKVDLVAEWGDPVRVELSQERYRALGL
                     KPGDGVFVSPVEMSLFAQDSPEP"
     gene            complement(9743..10585)
                     /gene="cysW"
                     /locus_tag="PKK95_04095"
     CDS             complement(9743..10585)
                     /gene="cysW"
                     /locus_tag="PKK95_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOC17423.1"
                     /translation="MSGPGDRSTAAAARGGGRAARWALTGLALLFLGCFLLIPLAAVF
                     GQALGEGVGAYWRAIADPDTLAALRLTALTAVVAVPLNTLVGLAAAWAIARFRFPGRR
                     LLVALIDLPFTVSPVVAGLVFVLLFGAQSALGPWLSEHGVRILFAVPGIILATLFVTA
                     PFVARELIPVMQEQGIEEEQAALALGATGWQTFWRVTLPNIRWALLYGVVLLNARAIG
                     EFGAVSIVSGHIRGQTNTAPLHVEILYNEYSFAAAFAVASVLAAAALVTLAAKAVIGR
                     RVGT"
     gene            complement(10582..11430)
                     /gene="cysT"
                     /locus_tag="PKK95_04100"
     CDS             complement(10582..11430)
                     /gene="cysT"
                     /locus_tag="PKK95_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOC17424.1"
                     /translation="MRGPSAPTQPRRRVLPGFRLTLGITTAYVGLLIVAPLAVLALRA
                     ARMGPSEVLAAVSSERVLAAAGLSFGASLAAALTNAVFGLLVAWVLVRYRFPGRRLVD
                     ACVDLPFALPTSVAGIALTAVYAPTGWLGAPLAAAGAQAAFTRLGVVIALTFVGLPFV
                     VRAVQPVLEDLDPEVEDAAASLGASRAQTFRRVLLPSILPALLTGFTLAFARALGEYG
                     SVIFISGNLPLRTEVVPLLIVARLEQYDYDGATAIAAVMLALSFGLLIAINGLQAWSR
                     RREVMP"
     gene            complement(11427..12488)
                     /locus_tag="PKK95_04105"
     CDS             complement(11427..12488)
                     /locus_tag="PKK95_04105"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOC17425.1"
                     /translation="MLKLPGRLWGPAALLAVALGAAWGWVAAASPAAPLPGMPGVQAV
                     TLLNVSYDPTREFYQEINAAFARHWRAKTGQAIAVRQSHGGSGKQARSVIDGLEADVV
                     TLALGYDIDAIARHGGTIAAGWQERLPHNSTPYTSVIVFLVRKGNPKGIAGWDDLVRP
                     GVSVIVPNPKTSGGARWNYLAAWAWARERAGGDETKAREFVARLFANVPVLDSGARGA
                     TTTFVQRGIGDVLVTWENEALLAIEKVGRDRVAVVVPPATILAEPPVAVVDRVVDRRG
                     SRRAAEAYLQFLYTPEAQEIAARHFYRPRLPEVMARHRARFPEVRTFTIDRDFGGWAQ
                     AQRTHFDEGGVFDRIFTRR"
     gene            complement(12546..13671)
                     /gene="prfB"
                     /locus_tag="PKK95_04110"
     CDS             complement(join(12546..13601,13603..13671))
                     /gene="prfB"
                     /locus_tag="PKK95_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110174822.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOC17426.1"
                     /translation="MLEDLVRRYEDLVKRSADLRGYLDRGAPGEELARLEALIAQPDF
                     WKDQAEAQRILQRRHRLEQEVQLIGQLKKREADLSVLVEWAGEGEDVAGELASALDEL
                     AREVEAAETRKLLGGEHDQKNAIVTIHPGAGGTESQDWADMLLRMYLRWTERRGFRRE
                     VMDYQPGDEAGIKSATLEIYGDYAYGLMSAEAGVHRLVRISPFDQAARRHTSFASVYV
                     WPELPDDVEVEIDEKDLRIDTYRSSGHGGQHINVTDSAIRITHLPTGIVVTCQNERSQ
                     GRNRDSAMRVLRARLYDLRMKEQQAKLEQLGGEKKDIAFGSQIRSYVLHPYQMVKDHR
                     TKEEVGDVNRVLDGDIDVFIKTYLKRKAAGTLGQAGADEE"
     gene            complement(13682..15232)
                     /gene="lnt"
                     /locus_tag="PKK95_04115"
     CDS             complement(13682..15232)
                     /gene="lnt"
                     /locus_tag="PKK95_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOC17427.1"
                     /translation="MDYSLAALSGVLLILSFPRFGHPAFAWIALVPLLVSLAPSWRPR
                     AGSGSLRALGLGFLAGAIHFAGTVYWTSAVMQQFGDLPLALAVLLMLLLASYMALYPA
                     IAALILHRLGRTFGTGALLFAPAAWVTTELGRAHLFTGFPWVLLGYSQATVLPLAQLA
                     SVFGVYGLSALLVLVNASLAWLVLSRSAARWRPLLVSLAVVVVVAAWGAARLREGSLV
                     RAGTPLEVALVQGNIAQEQKWDPSRARGILERYVEMTRQAASGGARFIVWPESSLPFY
                     FQEEPSGGDAVRRLAHDTRTFILVGSDQVERSDPPKFYNAAFLVTPDGTVGGIYRKMH
                     LVPFGEYVPLKKLLFFASPLVEAVSDFSPGEEIVMLPVAGHPVSTAICYEVVYPGLAR
                     GAVARGSQLLTTITNDAWYGRSSAPWQHFEQASLRAVEQGRYLIRSANTGISGIVDPY
                     GRVVARSNLFEQAIVEGEVRLLDGRTVYSRIGDLLAYVLALLALAAVLAGRLRSPVAG
                     EAGLERSR"
     gene            complement(15217..15879)
                     /locus_tag="PKK95_04120"
     CDS             complement(15217..15879)
                     /locus_tag="PKK95_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOC17428.1"
                     /translation="MSDEGFQEIHLGNKQLVFLAMTGCVVLVVAFLFGVLVGRGARAQ
                     EPAATMEVQADAEVTPPGHAADPAPVNASPSAAPLSVPEPIDDELSYPKRLEDGGPAN
                     DTLAAAKTSAPPPAPAAAPPSTAPAPAKPAAAPADKALAEPPGQGYAVQIVALRDRAK
                     AEAVARRLAGKGHRAYLVAPGARGSAMYRVVVGKFKSRQEAEQVKRRIEKEEQFKPWI
                     IR"
     gene            complement(15919..>16153)
                     /locus_tag="PKK95_04125"
     CDS             complement(15919..>16153)
                     /locus_tag="PKK95_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17429.1"
                     /translation="AEREKRAKIIHAAGELEASEKLSQAAHVIAVEPVTVTLRYLQTL
                     TEIASEKNSTIIFPLPLDLLHLLGGHAKQQDRE"
BASE COUNT         2375 a         5850 c         5573 g         2355 t
ORIGIN      
        1 ttgctcggcc cccgcgaggc gctggaggag gcgctcttca tgggactccg gctgtgtgac
       61 ggggtgtcgt gcgaggcgct cggcgcccgg tacggcgtgg acatctgggc cgtttacggc
      121 gagcggctcg aacccgccat cgacgcgggc ctgctgattc ggcagggaga atgcctgcgg
      181 ctcacgcggc cggggatgct gatggccaac gaggtaatgg ctgcttttct acggaccgac
      241 agtacggtag agtaacgccc tcaccggctt gtactcgtgt caaggaggcc tgcatgcgac
      301 tgacctggtt tgcgatcgtt acttgtcgac ggccgatgct ggctggtctg gtgctcgccc
      361 tagtgctggc gttcgggtcg ccgaccctga ccttcgccca ggaggagcag ggcgccccgc
      421 agaagccgac cgtggcgttc cagaacgacg ccggcttgat gatcctctac gtcaaggcgg
      481 acaagaccgc cgacttcgag gagttggcgg cgcgcctcaa ggagggtttg accaagatcg
      541 aggcgccaga ggtgaagcag caggccgaga gctggaagct gttcaagggc ccggtgggca
      601 acggcacggc cgtctacgtg atgctcgccg acccggtcgt gaagaacgtc gagtactggt
      661 tcctctcgct gctgtacaag gctttcccgg ccgacgcgca ggcgctgtat cagaagtgga
      721 ccgacgcgaa ggcggccaac ccgcccgcgg tcttcgacct cacgctcgtc accaagatgc
      781 agtaggcggg cggcccgcct cctcgcgacg agcgccccaa accgggagcg gtgcagttgc
      841 cgctccctgt ttttttgtac ggccgatcag aaccccaggt tggcacccgc gtagaggcgg
      901 tggtagcggt cgtgcagcgg gtcgccgcgc agcgagaaca gacggtagtc gagccgcagg
      961 cgcagcggcc cgagcagccg gatcttcgcg ccgccgccca ggttgacggc gaggctggtc
     1021 tcggaggagc cgccgagccg ctcgcggtag acgccggccc cggccgtgac gtagagctgc
     1081 gcgccgccca gggcaatagg ggtctgcagg aggccgttca ccgacccgac gcgcaagccc
     1141 ggctcgaggt ccccggcgtc ctcggcaatg tccgagtact cgaactcgaa gccgagcgcc
     1201 agcactcccc caccgatcgc cagccccctc gcctgccggg agcccggcgt ggtgctgagc
     1261 ccgaggaacg cggtgatgtc ggcgcgggcc ggcgcagccg cgccgagcca cgcgaagacg
     1321 acgagcgccg cgaccatccc tcgcctggcc atgcccacct cgcctccacg atccgttcca
     1381 gccgcccgta tcaggttccg gcccgtccgc ccggccacgc gacgtcggcg cgcgggtcgg
     1441 gtttcctgag ctgggcgcgc acctgggtgc cggccttgcg cggcaggacg gcgcgcttcc
     1501 cctgctgcac cttccacatc gccagcaggt cttccggccc cgcgttgggc gggcggtgcc
     1561 ggtagtcggc gccgtcgtac tcgaggaact cgcacatctc gtacaggcgc gagtgcatgc
     1621 ggaagccgac gcgcgccagc agcgagtcgg ggtcgccggc gtcctccttc tcctcgtagt
     1681 tggaggtgaa gacggtgagc cgccggtcgt tgtagcgcgt gctgacgatg aggttgaggg
     1741 tttcctcgac ccactccgac gacttctccg agccgatgtc gtcgagcacc agcaggtcgg
     1801 cgtccatcac cggccgcagc acctccgact cgttcgcacg gaccgacggg tcgtacgtgc
     1861 tgcggatcag gcgcagcagt tcgcgcacgt cgtagaagag cccgcgggcg ccgcgcgcga
     1921 ggatgacctg ccggaggacc gcgacggcga ggtggctctt gccgacgccg ggcggcccga
     1981 tgaggaacaa ccccttgtcc acgacgggga acgcgtcggc gaaacgccgc gcgcgggaga
     2041 gggcgttctg cagcttctcg ttcgggtagg tgacgaagct ctcgagcgtg cagtgctggt
     2101 agcggcgcgg gatgcgggcc tcggccagcc ggcgcgccgc gaccgcctcg cgccagcagt
     2161 cgcagcgcac ggcctcgcgg cggccctcgc gctcgaccac gcgccacctg gtatcgtcgc
     2221 agatcgggca gggcatggga cgcctattgt gccatgacgg accgcgaaga gtgacgtcaa
     2281 gatgcgaccg cgaagatcac aagattggca ctgtcaaaga tcgcaaagag ttgaccacaa
     2341 agatcacaaa gggtaagacg cagggtcgtc gggcgggcgc gaagcgcgcg gccgccgaag
     2401 acccgcggat gcgcgaggcc cactcctctt tgtgttcttt gtgttctttg tgttctttgt
     2461 ggttattccg tggggggcac gaacggttgt tcgcgcgcgt caggacttcg cgctggctcg
     2521 cggcctgccg cccgccttct cggcgacggg cgcctgctgg tcgggcggcg gcggcgtgga
     2581 acgcaccagg tcggagggcg ccgagcggac gagcgccgtg tgcaggcgct gctcccaatc
     2641 gtcgaggccg cgcgccagct cgcccttcac gtaggccatg aactcgttga cctcgtgctg
     2701 catctgctcg atcttccggc gcatgttgag gatgatctcg acgccggcca ggttcacgcc
     2761 gagatcgcgc gtgagcgaga ggatcgtctc cagctgctcg aggtcctcct ccgaatacag
     2821 ccgggtgttc ccctccgtgc gcgacggctt caacaatccc tcgcgctcgt agagccggag
     2881 cgtctgcggg tggatgctgt acttctgcgc cacggcgctg atcatgtagt aggccttgcc
     2941 gcctcgcctc gtcaccatgg ctccccctca gtccaaccgg cggcccgcgc cctccgccgc
     3001 ccgccactcg cggcggacgt cctcgtggtg gatccgcgcg aactcccgca acagctcctt
     3061 cgaccgctcg tcgagcagcg agggcaggac gagcttcacc tccaccagca ggtcgccgcg
     3121 acttccctgc cgcggcatgg gcagcccccg ctcccgcagg cgcagccgct gccccgactg
     3181 cgtcccgggc ggcacgcgca ccgtgaccgc tccctcgagc gtcggcacct cgatgcgcgc
     3241 cccgagcgcc gcctcgtgta tcgccaccgg cagcaccagg tgcaggtccg gtccctcgcg
     3301 ccggaacagc gggtgcggct cgacgtggac acgcacgaac aggtcgcccg gctcgccgcc
     3361 gcggcggccg gcgtgccccc cgcccgccac gcgcagctcg gcgccgtccg cgatgcccgg
     3421 cggcaccgcc accctcaccg tctcgccccg cacctcgacg ccctggccgc cgcagtggcg
     3481 gcaggtcatc gcggtcagct ggccgctgcc tccgcaccgc tcgcaaggcc tcgcgaacac
     3541 catgtggccg cgcgacgagc ggacgcgccc ggtcccgcgg caggccgggc agtcgcccgc
     3601 cggcgcatgc acgacgcccc ggccaaggca cgcccggcac gccacgtgcc gcgtcagcgg
     3661 cacctgccgc tcggtgcccg tcagcacctc ggcgagcgag acgctgaagg cggcgtgcag
     3721 gtgggccccg cgctgcggcc ccgcctcgcc gcccccgagc gtcctggtca gcacgtcggc
     3781 gaacagctcg ccgaaggtgg aggcctcggc gccaaccgac gcctgcacgg agaagtcgaa
     3841 cccctcgaag ccgtacgact gcgccggctc cgcgggctcg agcggaacgc cggcgtcgta
     3901 ggcgcggcgc cgctcggggt cgttcagggt ctcgaacgcc cgcgtgatca gggcgtaccg
     3961 ggcctgggcc tcgcggtcgc cgggattgac gtccgggtga taccgccggg ccaggcggcg
     4021 gtacgcacgg cggatctctc cgccgcccgc tccccgccgc accccgagaa tctcgtacag
     4081 gtccatgggt tcacatcagc cggccgcaga cagcctgtcc gctgtctgct gtcggctact
     4141 tcttgtcgtc caccacctcc gcgtcgatga cgtcgtcgct gccggtgcgg ccctcgccgc
     4201 cggcagacgc cccggcgccc gcctcctcac cgggctgggc gcccggcccg cccggctgcg
     4261 actgccggta gagcgcctcg gccatcgcgt gcgacgcctt ctgcaggtcg tccatcgcct
     4321 tgcgaatcgc tgacacgtcg tcgccctcgg cggccttccg gagcgcggcg atcgacgact
     4381 cgatgcgcga cgcgtcaccg gcggccaccc gctcgcggtt ctcgttgagc gtcttctcga
     4441 tctggtagat caggccgtcc gcctggttgc gcgcgtcggt cacctcgcgc cgccgcttgt
     4501 cctcgtcggc gtgcgactcg gcctcgcgca tcatgcggtc gacctcgtcc ttgctgaggc
     4561 cgctcgacgc ggtgatcgtg atcttctgct ccttgttggt cgccatgtcc ttggccgaca
     4621 cgttgacgat cccgttggcg tcgatgtcga aggtgacctc gatctgcggg acgccgcgcg
     4681 gagccggcgg gatgccgacc aggttgaagc ggccgagcgt gcggttgtcg cgggcgaact
     4741 cgcgctcgcc ctgcatcacg tgcacctcga cctgcgtctg gttgtcggac gcggtcgaga
     4801 acatctcgct cttgcgcgtc gggatcgtcg tgttgcgcgg gatgagcttg gtcatcaccc
     4861 cgccaagcgt ctcgatgccg agcgagagcg gcgtcacgtc gagcagcagc aggtccttga
     4921 cctctcccgc caggacgccc gcctggatgg ccgcgccgac agccaccacc tcgtcggggt
     4981 ttacgccgcg gtgcggctcc ttgccgaaca gctccttgac gacctgctgc acgcgcggga
     5041 tacgggtcga gcccccgacg agcaccacct cgtcgatctt cgaggcgtcg agcccggcgt
     5101 cggcaagcgc gcggcgcacc ggcccgacgg tcttctggat caggtcctcg accagttgct
     5161 cgaacttcga gcgcgtcagc cgcatcgtca ggtgcttggg gccggtctgg tcggcggtga
     5221 tgaacgggag gttgatgtcg gtctcggtga ccgtcgacag ctccatcttc gccttctcgg
     5281 ccgcctcctt cagacgctgg agcgccatcc ggtccttgcc gaggtcgatc ccctcgctct
     5341 tccggaactc cgtgatgatc cagtcgatga tccgctggtc gaggttgtcg ccgcccaggt
     5401 gggtgtcgcc gttggtcgac ttcacctcga caaccccctc ccccacctcg aggatcgaga
     5461 tgtcgaaggt accgccgccg aagtcgtaga ccgcgatcgt ctcgtccttc ttcttgtcga
     5521 gcccgtaggc cagcgcggcc gccgtcggct cgttgacgat ccgcttcacc tcgaggcccg
     5581 caatctggcc ggcatccttg gtggcctgcc gctgggcgtc gttgaagtag gccggcaccg
     5641 tgatgaccgc cgcggtcacc gtctggccga ggtaggcctc ggccgcctgc ttcagcttct
     5701 gcaggatcat cgcggagatc tcgggcggcg agtactcctt gcccatcgcg accacccgga
     5761 cgtcaccccc ttggcccgcc acgaccttgt aggggaccat cgtcatctcc tcggtcacct
     5821 cgtcgtagcg ccggcccatg aaccgcttga tcgagaagat cgtgttctcg gggttggtca
     5881 ccgcctggcg cttggccacc tggccaaccg ggcgctcccc cgtcttcgtg aacgcgacaa
     5941 cggacggcgt gagccgcccg ccctcggggt tggtgatcac ggagggctcg ccgccctcca
     6001 tgacggccac gaccgagttg gtggtcccga ggtcgattcc gatgattttg ctcatagcgc
     6061 ttcccatccc ccgaaagctg atctgcggga ccgccggtcc cgtaacacgc caatctgcgc
     6121 cgaccctatg cagtaaacac tatagaaata tgagtgcgaa tctgtcaagc cggacaacaa
     6181 cccgctttca ccaacgcagg ccgcgtctcg gaggcaggat ggtttcgggg caggctgcgg
     6241 cttcggcggg caggctgcgg cttcggcggc gccgtgggtc gccggcaccc accagcacgg
     6301 cactcttgat aagatcgcgt cgtggcccac gtcgttcttc gcgtcgcccg gcatgcgggc
     6361 atcgtggcgc tgttcgtgct cgccgccgtc ctcggggtgg tgacgggcgt gttcttcgcc
     6421 tacgccggcg atctgcctcg catctcggcg ctcgacgact acgccccgaa cacgatcacg
     6481 cgcgtgctgg cggccgacgg gcaggtggtg gccgagttcg cgaccgagcg gcgggtcgtg
     6541 gtgggctacg acgacatctc ggcgcggctg cgccaggcga tcatcgcggc cgaggatgcc
     6601 gggttctacc gccacgtcgg cgtcagcatg acgcgggtgc tcatcaccgc ggtcgaggac
     6661 gtgatcaagc gccggatggc gggcgccagc acgctgacca tgcagctggc gcgcgggctg
     6721 ttcctgaccg acgagaagac gtgggagcgg aagatcaagg aggccctgct ggcgctccag
     6781 atcgagaagc ggtacaccaa gcgcgagatc ttcaccttct acgcgaacca gatctacctc
     6841 ggtcacgggg cgtacggcgt cgaggccgcc tcgcggatgt acttcggcaa gcgatccaag
     6901 gacctgacgc tcgaggaagc ggcgctgatc gccggcatca tccagctgcc gcagcgccag
     6961 agccccttcg tcgacgtgcg gcgcgccacc gggcggcgca actacgtgct gcgccagatg
     7021 gccgaggaga agtacatcac gcgcgaggag gccgaggcgg cttcccgcca gccggtggtg
     7081 gcgcgcggcc aacccagggc cgacgagtcg atcgcgccgt tcttcgtcga ggaggtgcgg
     7141 aagtatttgg agcggaagta cggggcgcgg cagctgtacg agaaggggct ctcggtggag
     7201 acgtcgctcg atccgcagct ccagctggct gccaaccagg cgctcgacga ggggctgcgg
     7261 gcgctcgacc ggcgccgcgg cttccggaag cccgcccgca acctggtctc cgagggcgtg
     7321 gacctcgcgc ggtacacgga cgaccggtgg agccggcctc gcgcggtcgg ccaggtgctg
     7381 ccggccgtgg tgatcgccgc gaacccgcgg ggcgccagga cggcgcgggg cggcgcaacc
     7441 gcccctggcg gggcattggc tcccggggcc gtccgcgtcc gcatcggcga gcagacggcc
     7501 gacctcgatc gccaggcgat cgcctggacg cgccgggcga acgcgagcga cttcttgcgc
     7561 gtgggcgacc tcgtcgaggt cagggtgcgt gccatcgacc ccgccactgg ggcgctcagc
     7621 gtcgtgctcg accaggcgcc gatcgtggac ggcgccctgc tcgccatgga caaccggacc
     7681 gggcaggtcg tggcgatggt cggcggcctc agctttaccc gcagcaagtt caaccgggcc
     7741 acgcaggcct cgcgtcagct cggatcggtc ttcaagccgt tcgtctacac ggtggccatc
     7801 gatcgcgggt tcaccccggc cagcgtgctg ctcgacgagc cggtggccta cgacgtcggg
     7861 cccgggcagc cgccgtacac gccgcgcaac tacgacggca ccttcgaggg gccgatcacg
     7921 ctgcgccggg cggtcgagca gtcgcgcaac gttccggccg tccgcaccat cgaccaggtc
     7981 ggacccgggc aggtggtgga ttacgttcgc cggttcggct tcgaggcgtc gttcccgccg
     8041 gtcctctcgc tggccctggg cgcgggagag gcgaccctgc tcgagacggt ggcggcctac
     8101 gccgtcttcc cgaaccaggg catccgcatg cagccctacc aggtgctccg catcctcgac
     8161 cgcgacggca acgtgctcga ggagaaccgc ccgcagccgc gcgaggcgct ccgcgccgac
     8221 actgcgtacg tgatgacgaa cctgctgcgc ggcgtggtgc agcgcggcac cgccgcctcg
     8281 gccgcgtcgc tgcagtggcc cctcggcggc aagacgggga ccaccaacga cttcaccgac
     8341 gcctggttca tcggcttcga cccgaacatc acggtcggcg tgtgggtggg ctacgacgac
     8401 aagaagccgc tcggcccggc cgagaccggc gcccaggcgg cgctgccgat ctggaaggcg
     8461 ttcatgaagg cctacatcga cgcgcgcggc gaccgccaga acccgcccgc cttcgaggct
     8521 cctggcaaca tcgtgttcct gaacgtagac cgggccacgg gccacccggc cgaagccgac
     8581 gcccccaacg tgatctccga ggcgttcatc gccggcaccc agcccggggg ctggcagtaa
     8641 gaaggctcaa gactcaaggc tcaaggcggg gctcacggct cggggctgtc ctgcgcgaag
     8701 aggctcattt ccacgggact gacgaacacc ccgtcgcccg gcttcagccc gagcgcccgg
     8761 tagcgttcct gtgacagctc gacgcgcacc gggtcgcccc actccgccac caggtccacc
     8821 ttcacgagcg gcccggccgg gttgacgtgg cgcacccgcg ccctgaactg ctgggccccg
     8881 ccttcctcga gcctgatctc gagctggtgc ggccggatgt agagggtggc gagccgcgcc
     8941 tcgcgcacgt cttcccggtg cggcacctcg aaggccagcc cgccgatgta ggccttgccg
     9001 tgctcgatcc ggaatcggaa caggttgacg tcgccgagga agtcgaccac gaacgggctg
     9061 accgggtgct ggtagacctc ggcgggggtc cccacctgct cgatgcgccc ctggttcatg
     9121 acgacgaccc ggtcggccgc ctcgagcgcc tcttcctggt cgtgggtgac gaagatgctg
     9181 gtgacgtgca gctcgtcgtg cagccggcgc agccagcgcc gcagctcctt gcggaccttc
     9241 gcgtcgagcg cgccgaacgg ctcgtcgagc agcagcaccc gcggctcgac ggcgagcgcg
     9301 cgcgccagcg ccacccgctg tcgctgcccg cccgagagct gggcgggcag gcggtgggcc
     9361 atcgcctcga gctgcaccag ctcgagcagc cgctgcacgc gccgctcgat ctcggggcgg
     9421 gccgggcgcg tctcgcgcgg ccgcacccgc agcccgaagg cgatgttctc gaagaccgtc
     9481 atgtggcgga agagcgcgta gtgctggaag acgaacccca cgcggcggtc ggcggccctc
     9541 cggttcgtgc tgtcctcgcc gtcgaacagc accgacccgg cgtccgggat ctccagcccc
     9601 gcgatcaccc gcagcagcgt cgtcttgccc gagcccgagg ggccgagcag cgccatcagc
     9661 tcgccggtct gcacgagcag atcgacccgg tcgacggcca cgaactggcc gaaccgcttg
     9721 gtgacgcccc tgacctcgat gctcatgtcc ccacccgcct gccgatcacc gccttcgccg
     9781 ccagcgtcac gagcgccgcg gcggcgagca cggaggccac cgcgaaggcg gccgcgaagc
     9841 tgtactcgtt gtacaggatc tcgacgtgga gcggggccgt gttcgtctgg ccccggatgt
     9901 gccccgatac gatcgagacg gcgccgaact ccccgatcgc ccgggcgttc aacagcacga
     9961 ccccgtacag caaggcccac cggatgttcg gcagcgtgac ccgccagaag gtctgccagc
    10021 cggtggcccc cagggcgagc gccgcctgct cctcctcgat gccctgctcc tgcatgaccg
    10081 ggatcagctc gcgggcgacg aacggcgcgg tcacgaacag cgtcgccagg atgatacccg
    10141 gaacggcgaa gaggatgcgg acgccgtgtt ccgacagcca ggggcccagt gcgctctggg
    10201 cgccgaacag caggacgaag acgaggcccg cgacgaccgg cgacacggtg aacggcaggt
    10261 cgatcaacgc gaccagcagg cggcgcccgg ggaaccggaa gcgtgcgatg gcccaggccg
    10321 ccgccaggcc gaccagcgtg ttcaggggca ccgccaccac cgcggtcagg gcggtcagcc
    10381 ggagagccgc gagcgtgtcg gggtccgcga tggcgcgcca gtacgcgccc accccctcgc
    10441 cgagcgcctg cccgaacacc gcggcgagcg ggatcagcag aaagcagccg aggaagagca
    10501 gcgcgagccc ggtgagcgcc caccgcgccg ccctcccgcc gccgcgcgcc gccgcggccg
    10561 tggagcgatc gcccggccca ctcacggcat cacctcgcgg cggcggctcc acgcctgcag
    10621 gccgttgatc gcgatcagca ggccgaacga cagcgccagc atcaccgcgg caatcgccgt
    10681 ggcgccgtcg tagtcgtact gctcgagccg cgcgacgatc agcagcggga cgacctcggt
    10741 gcgcagcggc aggttccccg agatgaagat caccgacccg tactccccga gcgcccgggc
    10801 gaacgccagg gtgaatcccg tcagcagcgc cggcaggatg gacggcagca ggacgcggcg
    10861 gaaggtctgc gcgcggctgg cgcccaggct cgccgccgcg tcctccacct cggggtcgag
    10921 gtcctcgagt accggctgca ccgcccggac gacgaacggg aggccgacga aggtcagggc
    10981 gatgacgacc ccgagacggg tgaaggcagc ctgcgcgccc gccgccgcca ggggcgcccc
    11041 gagccatccc gtcggcgcgt agaccgcggt gagcgcgatg ccggccaccg aggtcggcag
    11101 cgcgaacggc aggtccacgc aggcgtccac caggcgacgg cccgggaagc ggtagcggac
    11161 cagcacccag gcgaccagca gcccgaagac ggcgttggtg agcgccgccg cgagcgaggc
    11221 gccgaagctc aggcccgccg ccgccagcac gcgctcgctc gaaacggcgg cgagcacctc
    11281 cgacgggccc atcctcgccg cccgcagcgc cagcaccgcc agcggcgcca cgatcagcag
    11341 cccgacatag gctgtcgtga tgccgagcgt gaggcggaag cccggcagga cgcgccggcg
    11401 cggctgcgtg ggtgcggaag gccccctcat cgccgggtga agatccgatc gaagacgccg
    11461 ccctcgtcga agtgagtgcg ctgcgcttgc gcccacccgc cgaaatcgcg gtcgatggtg
    11521 aaggtgcgca cctcggggaa gcgggcccgg tgccgggcca tcacctcggg aaggcgcgga
    11581 cggtagaagt gccgggcggc gatctcctgc gcctcgggcg tgtagaggaa ctgcaggtac
    11641 gcctcggcgg cgcggcggct cccgcggcgg tccaccaccc ggtcgacgac cgcgaccggc
    11701 ggctcggcga ggatcgtcgc cggcggcacc accacggcga ccctgtccct gccgaccttc
    11761 tcaatcgcca gcagcgcctc gttctcccac gtcacgagca cgtcgccgat gccccgctgc
    11821 acgaaggtcg tggtcgcgcc gcgggcaccg gaatcgagca ccggcacgtt ggcgaagagc
    11881 cgcgccacga actcgcgcgc cttcgtctcg tcgccgccag cccgctcgcg cgcccaggcc
    11941 caggccgcca ggtagttcca ccgcgccccc cccgacgtct tcggattcgg cacgatcacc
    12001 gagacaccgg gccgcacgag gtcgtcccag cccgcgatcc ccttggggtt ccccttccgg
    12061 accaggaaca cgatgaccga cgtgtagggg gtgctgttgt ggggaaggcg ctcctgccac
    12121 cccgcggcga tcgtgccgcc gtggcgcgcg atggcgtcga tgtcgtaccc gagcgccagc
    12181 gtgacgacgt cggcctcgag gccgtcgatc accgatcggg cctgcttgcc cgatccgccg
    12241 tgcgactgcc ggaccgcgat tgcctggccg gtcttcgccc tccagtgccg ggcgaacgcc
    12301 gcgttgatct cctggtagaa ctcccgggtg ggatcgtagg agacgttgag gagcgtgacg
    12361 gcctgcacgc ccggcatccc ggggagcggc gcggcgggag acgcggcggc gacccagccc
    12421 cacgccgccc cgagcgcgac ggccagcagg gcggccggcc cccacagacg gcccggcagt
    12481 ttcagcaccg gcatagtcta gcagcctgcg gcgccacccg ggcacaggct gcgggaacgc
    12541 cgccgctact cttcgtccgc ccccgcctgc cccagcgtcc cggccgcctt ccgcttcagg
    12601 tacgtcttga tgaagacgtc gatgtcgccg tccagcaccc ggttgacgtc accgacctcc
    12661 tccttggtgc ggtgatcctt caccatctgg taggggtgca ggacgtagct gcgaatctgg
    12721 ctcccgaagg cgatgtcctt cttctccccg ccgagctgct cgagctttgc ctgctgttcc
    12781 ttcatccgaa ggtcgtacag ccgggcgcgc agcacgcgca tcgccgagtc gcggttgcgc
    12841 ccctgcgatc gctcgttctg acaggtcacc acgatcccgg tgggcaggtg ggtgatgcgg
    12901 atcgccgagt cggtgacgtt gatgtgctgc cccccgtgac cgctcgaccg gtaggtgtcg
    12961 atgcgcaggt ccttctcgtc gatctccacc tccacgtcgt cgggcagctc gggccagacg
    13021 tagaccgacg cgaaggaggt gtggcggcgt gccgcctggt cgaagggcga gatccgcacc
    13081 aggcggtgga cgccggcctc ggccgacatc aacccgtacg cgtagtcgcc gtagatctcg
    13141 agggtggcgc tcttgatgcc cgcctcgtcg cccggctggt agtccatcac ctcgcgccgg
    13201 aagccgcgcc gctcggtcca tcgcaagtac atgcggagca gcatgtcggc ccagtcctgc
    13261 gactcggtgc cgccggcccc ggggtggatc gtgacgatgg cgttcttctg gtcgtgctcg
    13321 ccgcccagca gcttgcgcgt ctcggccgct tcgacctccc gcgccagctc gtcgagggcc
    13381 gaggccagct cgcccgccac gtcctcgccc tcccccgccc actcgacgag caccgagagg
    13441 tcggcctcgc gcttcttcag ctggccgatc agctgcacct cctgctcaag gcgatgccgc
    13501 cgctgcagga tccgctgcgc ctcggcctgg tccttccaga aatcgggttg cgcgatgagg
    13561 gcctcgagcc gcgccagctc ctcgcccggg gctccccggt caaagatatc cccggagatc
    13621 ggccgaccgc ttcaccagat cctcgtaccg cctgaccagg tcttcgagca cgatcgccac
    13681 gtcatctact cctttcgagg cccgcctcac cggccaccgg ggagcggagg cggccggcca
    13741 gcaccgcggc cagcgccagc agggccagca cgtacgcgag caggtccccg attcgagagt
    13801 acaccgtccg cccgtcgagc aggcgcactt ccccttcgac aatcgcctgc tcgaacagat
    13861 tcgagcgggc caccactcgc ccgtacgggt ccacgatgcc gctgatcccc gtgttcgccg
    13921 agcggatcag gtagcgtccc tgctcgaccg cgcgcagcga tgcctgctcg aagtgctgcc
    13981 acggcgccga cgaccgtccg taccacgcgt cgttcgtgat ggtggtcagc agctggctgc
    14041 ctcgcgccac cgcgccgcgc gcgaggcccg ggtaaaccac ctcgtagcag atcgccgtgc
    14101 tgaccgggtg cccggcaacc gggagcatca cgatctcctc gcccggcgag aagtcggaca
    14161 ccgcctcgac cagcggcgag gcgaagaaca gcagcttctt gagcgggacg tactcgccga
    14221 acggcacgag gtgcatcttg cggtagatgc ctcccaccgt gccgtcgggg gtgacgagga
    14281 acgccgcgtt gtagaacttc gggggatcgg atcgctcgac ctggtcgctg ccgaccagga
    14341 tgaaggtccg ggtgtcgtgc gccaggcggc ggaccgcgtc gccccccgag ggctcttcct
    14401 ggaagtagaa cggcagcgac gactcgggcc agacgatgaa gcgggcgccg cccgaagcag
    14461 cctggcgggt catctcgacg taccgttcga ggatgccccg cgcccgggag ggatcccact
    14521 tctgctcctg cgcgatgttt ccctggacga gcgcgacctc gagcggggtg ccggcgcgca
    14581 ccaggcttcc ctcgcgcagg cgggcggctc cccacgccgc gacgaccacg acgaccgcga
    14641 gagagacgag cagggggcgc cagcgggcgg cgctccggct cagcaccagc cacgccagcg
    14701 acgcgttcac caggacgagc agcgccgaga gaccgtagac gccgaacacg ctggccagct
    14761 gtgcgagcgg cagcacggtc gcctggctgt agccgagcag cacccagggg aagccggtga
    14821 acagatgggc ccgccccaat tcggtcgtca cccacgcggc cggtgcgaac agcagggccc
    14881 cggtcccgaa cgtgcggccg agccgatgca ggatcagcgc ggcgatggcc gggtagagcg
    14941 ccatgtacga ggccagcagc agcatcagca ggacggccag cgcgagcggc agatcgccga
    15001 actgctgcat cacggccgac gtccagtaga cggtgcccgc gaagtgaatg gcgccggcga
    15061 ggaagccgag gccgagtgcg cggagagacc cgctgccggc gcgcgggcgc cacgagggag
    15121 ccaggctgac cagcaggggt acgagcgcga tccacgcgaa ggcgggatgc ccgaaccgcg
    15181 ggaagctcag gatcagcagg acgccggaga gggcggctag cgaataatcc agggcttgaa
    15241 ctgctcttct ttctcgatcc ggcgcttgac ctgctcggcc tcctgccggc tcttgaactt
    15301 cccgaccacc acgcggtaca tcgcgctgcc gcgcgcaccg ggggcgacga ggtaggcgcg
    15361 atgccccttg ccggcgaggc gccgggccac ggcctctgcc ttggcccggt cgcgcagcgc
    15421 cacgatctgc accgcgtagc cttgtcccgg cggctcggcg agcgccttgt cggcgggagc
    15481 cgccgcgggc ttcgcgggag ccggtgccgt cgaggggggc gcggcagcgg gggcgggcgg
    15541 cggggcggac gttttggcgg ccgcgagcgt gtcgtttgcc ggcccgccgt cctcgagccg
    15601 cttgggatag ctcagctcgt cgtcgatcgg ctcggggacc gagaggggcg ccgccgaggg
    15661 cgaggcgttg acgggcgcgg gatcggcggc gtggcccggg ggcgtcacct cggcgtcggc
    15721 ctgcacctcc atcgtcgcgg ccggctcctg cgcccgcgcg ccgcgcccca ccaggacgcc
    15781 gaagaggaac gccaccacga gcacgacgca gcccgtcatg gcgagaaaca cgagctgctt
    15841 gttccccagg tggatctcct ggaacccctc gtcggacatc gaccaccttt cgccggacgc
    15901 gcttcccggc tgaagcttct actcccgatc ctgctgcttc gcgtgccccc cgaggaggtg
    15961 cagcaagtcc agcggcaacg ggaagatgat cgtggagttc ttctccgagg cgatctcggt
    16021 gagggtctgc aggtagcgca gcgtgaccgt gaccggctcg acggcaatca cgtgggccgc
    16081 ctgcgacagc ttctcggagg cctccagctc gccggcggcg tggatgatct tagcacgctt
    16141 ctcacgctcc gcc
//
LOCUS       DAOPQX010000045        15920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4383_length_15920_cov_4.687551, whole genome shotgun sequence.
ACCESSION   DAOPQX010000045 DAOPQX010000000
VERSION     DAOPQX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15920
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4383_length_15920_cov_4.687551"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..2812)
                     /locus_tag="PKK95_04130"
     CDS             complement(113..2812)
                     /locus_tag="PKK95_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17430.1"
                     /translation="MGSLLRRLLFLFRRDRLDRDLDEELRFHEEMTAQSLADDMGGDE
                     ARAAARRRVGNPLRLRERAREPWTFPTIETLAQDVRHALRLMRRDPAFTITALATLAL
                     GIGLSTAIFSVAYGVLWRPLPYPNPDRLVILSAAQQTGKGVKTIWTWAPVSYAALGPR
                     LDAFDHLAAYVSIEAQLTGRGEPLQVPAVEVSPNFFTTLGVGPAHGRTFLAGAGAADD
                     DRAVIVSDRLWRSSLKADPAFVGQSITINGLPRTVVGVLPRGFSFRPVVRAGALPEAD
                     VFLPSRWPGDAGRSAFLYLLGRMKPGVNKERAETELTALVNDPSIVAPGALAPEGVLA
                     PNVRTIARTAGLQEYGAGPVRTLLLIVLGAVAFVLLIACVNVANLQMARLTARRGELS
                     VRMALGAGRRRVVRQLLTEALVLSLGGAAFGVLLARIAIDVTLPLVPQAVLPRLGGIE
                     IDGGVMAFSIALTLVSGLLIGLVPALRVSGTALADGRALHAGEARTTGDGPGERLRTL
                     LVVAQVAMTLVLLIGAGLLMHSFVRLTSVSPGFEASGRDGVVSTVRVTLPERLFDRPE
                     RIHAFARGVLDRVRYLPGVTAASLVNSPPFGRMFIQGDFSIEGQPKPNLAAGRPKIGA
                     GYFQTLGIPLLAGREFTAGDTAEAPRVAIVSERIVRECLPGGPGEALGRRVRLDGLSD
                     GSEWLTVVGVVADIRHMGLDRDVQPMIYVPFQQDRSDFVRFVSFVARTSNSAAVVEGI
                     RAEIRRAAPDLAITATVTMDEAVAASVAPPRFRMLVLGLFALAATLIATCGIYGLMAY
                     AVTQRRREIGVRVALGAERSDVLRLVLGRALQVVTAGAVLGLAGAIAVTRVLRTFLFG
                     VTPTDPLAFTVVTAALVAVGLTAAWVPARRATRIDPVEALRAE"
     gene            complement(2852..3190)
                     /locus_tag="PKK95_04135"
     CDS             complement(2852..3190)
                     /locus_tag="PKK95_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC17431.1"
                     /translation="MNATKDKNELLPGTLEMLILKTLSIEPMHGYGIAQHIRVISRAV
                     LTVEEGSLYPALQRMLVKGWVSAEWKVSANNRRARFYTLTPAGRKQLGVEESKFSQMF
                     GAIMRVMKTT"
     gene            complement(3499..5760)
                     /locus_tag="PKK95_04140"
     CDS             complement(3499..5760)
                     /locus_tag="PKK95_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOC17432.1"
                     /translation="MTHIAAREGSDTAFDRAVAAVRAGEAPEGQAKALYDRLTEDERL
                     NLLDGDTPFWEGMRSMREEGYNARPYVHGAIGRLGIPGTRFVDGPRGCVAGKGTAFPV
                     SMARGATWDVGLEERVGEVIGREIRAQGGNFFGGVCINLPRHPAWGRIQETYGDDPCH
                     LGEFGAALTRGAQKYVMACAKHYALNSMENARFTVDVTIDEATLHDVYLPHFKRAVDE
                     GVAAVMSAYNSVNGEWAGQNEYLLTEVLRNQWRWSGITVSDFIWGLRDAAKSLNAGLD
                     LEEPLAQQRATHLRRQLAAGETSWDKVERAGVRLLAAQLRSYATRAQGPFGLDLMANE
                     EARALSRTVAARSMVLLKNDEVGGAPLLPLTPAAVSSIAVVGRLADAPNMGDHGSSDV
                     RAPGYVSPLAGITAAFPGAKIAAITDGDAAAARAIARSAEVAIVIAGYDARDEGEYVG
                     ADTMRRPELLALFPPLPPSGGRPQAAAGGGSRPAGPGPNPVMTAGDQFGGDRASLALR
                     AQDEEIIRAVAAVNPRTIVCIVAAGAVMMEAWRREVPAILMMWYAGMEGGRALADVLT
                     GVHNPSGRLPFSIPVSSDHLPPFDRTATKVTYDRFHGQRLLDKLGVKAAFPHGFGLSY
                     TTFGIGEVAVIERGPAGMRLAATVTNTGRRDAHHVVQVYGRCRQGGYPGELMLIGFAA
                     APVAAGKSVRVEVPVSFTPLARWDASKKTRVMPDPADVDLEVGSYAHDPSAVVLAGSG
                     LRHGDPAHSAASR"
     gene            complement(5877..7274)
                     /locus_tag="PKK95_04145"
     CDS             complement(5877..7274)
                     /locus_tag="PKK95_04145"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC17433.1"
                     /translation="MARMTDERSGASREPMAAAVDSLMPQLTAELVRLSRIPSISEAG
                     FPPEPMHEAHLVVSELLRDAGVNVSQLDLPNTYPIVTGEIPAPPGAPTVLLYGHYDVV
                     PPGDESKWLSPPFEPTERDGALFGRGTSDSKANVLAHVGALRAHGGKPPVGIKVVIEG
                     QEEVGSAFNTYPPLDADRFRCDAMVIADMGSVRPGVPTLTVALRGTAVVTVEVRTLAG
                     PKHSGQYGGAAPDALIALLHGLATLHDVKGDVTVEGLRRDAWTGASYDEREFRALAEI
                     MPGLPLFGTGTLGERLWSGPAITVTGLDALPVDKAVNAVVPQARARINLRVHPEQNAA
                     EAQNALVRHLRGLRPFGIALDVTPGAIGNGFAASTSGPAYDAARAALSSAWGGETVSV
                     ATGGSIPLVNALHEAVPQAEILLLGATDGFSNIHAPNERVLLDEFRKTVIAEAEIFRE
                     YAERWGGRRAAAAGR"
     gene            7566..8594
                     /gene="glsA"
                     /locus_tag="PKK95_04150"
     CDS             7566..8594
                     /gene="glsA"
                     /locus_tag="PKK95_04150"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HOC17434.1"
                     /translation="MPVPARDTNRPAAAAPFVSTGRLPPPERVRSWVLEAHEKYRLNG
                     EGQVSQVYPALARVRPDLFGVCVVAASGGVYAAGDAGHEFAIMSVSKPFVLALVCEAL
                     GPGEVREKVGANATGLPFNSLAAVEESPDGRTNPMVNAGAIATTSLVPGATPEARWEF
                     IHEGLSRFAGRVLTLGEEVYASASTTNQRNQALALLLQSRDRIYSPPSEATDLYTRQC
                     SLNVTAKDLAVMGATLADGGVNPLTGQTVVGTATCHYTLAAMSTAGLYETSGEWLYDI
                     GLPGKSGIGGGIVAVSPGKGGLGTFAPPLDGAGNSVKGQLVARFLSHQLGLDLFASRP
                     ADSSDPRA"
     gene            8826..9902
                     /locus_tag="PKK95_04155"
     CDS             8826..9902
                     /locus_tag="PKK95_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOC17435.1"
                     /translation="MADANYWGAFLSALLPTLLIAWLAAWLARRLARNATLAVVGDRL
                     RADSPVIRGPLRLVAAAAFVLVVALLLFPAMELAGFRPTVGRKAREMAVWLFGGGLRV
                     GMIALIAYSLRRATLLYVQRFEDELTAGATIGELERNRRARTLGSMVNKAITGLIVGV
                     AGVLILDEVGVDVAPILTGAGIAGIAIGFGAQTLVRDVLSGFFLILEDQVRVGDVAEI
                     NGTTGLVEQLNVRTIVLRDDRGTVHVVPNGAIVTLANHSKDFSRYVIDVDLPYAEDPD
                     RVAEIARDVDREMRSDPKFKILILEPIEIVGVVAYSQWSMQWRIRMKTVAQRQWVVGR
                     EFRKRLRKALNLHGIEVPYPVFRP"
     gene            10014..10898
                     /locus_tag="PKK95_04160"
     CDS             10014..10898
                     /locus_tag="PKK95_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17436.1"
                     /translation="MPRELAEKLKDSIVSTVRGTGEIVNAVTETVSDTLVTALQGTGS
                     VGKALTGAVADVASGAIEGTSQVGGDLGKAAKGAVIGVLQGTKRVGAEAVDTIGSTVQ
                     TLVKTTVEVGGDAGSAARQAMEGTIEGARSIKLAGVEAVTAAAAGAVQGVGRVGVSAT
                     TTTADVARELISGAANVGGDLGSAAKGTMLGVLRGTKELTAQATDTVGATAGSLVRAT
                     AGVGGDIAATARAAVDGTIQGAREVGVDAAGAASAAATGALAAAGDIGTAAVEQVERA
                     ATGVISGVKVVVTSPFRG"
     gene            complement(10917..11270)
                     /locus_tag="PKK95_04165"
     CDS             complement(10917..11270)
                     /locus_tag="PKK95_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOC17437.1"
                     /translation="MRVIGVELDKDDRKLIRLKLGAKLGKFATSIERVSVRVTDANGP
                     RGGVDQVCRVKVVLSGLPSVVVERRHRSLGAAIDAALQATEGAVRSSVRRRRMKPRRR
                     RKPASGTRTRPPSPA"
     gene            complement(11686..13068)
                     /locus_tag="PKK95_04170"
     CDS             complement(11686..13068)
                     /locus_tag="PKK95_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOC17438.1"
                     /translation="MAPALVILALMFAFAALIRRWSRPLRARFIPTAVIGGFLVLALG
                     PDGVGRVTGGQGAFSAETLAVWKALPGLLINVMCAALLLGEHLPPLKTVWTISRSHVV
                     LASIMSAGQFAVGSILVLLVLGPLFGLNSKAGSIIEMSFAGGHGTLAGLTPVLVQYGA
                     EELLELGLGLATIGMVTGIVIGTALVNYAITSPRIPVARQQPTSPEEDYDIDYHLPGP
                     DDVPIDEWRGMTQVTAAAVFIGVSIAVGYVLLELFRVTFSLLGSSFFDKFPLFPFTIL
                     GGAIVQVCAVRFNFEWAVNRLAVEGLGGLAVDGIVICAIGTLSLAALGSQLGPLIVLA
                     VASVAWSVFVTLVISPRIFQRNWFEHGLAEFGESQGNVATGFVMVDMADPDRRTDVVR
                     AYSYRQLITRPVLGGGFITALSVPLIASWGLPLFTIVTATATIALTMWGMRRATAGAG
                     LPAGSPPRAG"
     gene            13246..14163
                     /locus_tag="PKK95_04175"
     CDS             13246..14163
                     /locus_tag="PKK95_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOC17439.1"
                     /translation="MQPPTLRSRTTPNSFAGLLRAAWQEYERDHARYFASAMVYHALV
                     SLLPLLLLVLAALGLFLRFSTLAAGVEEQVLQAVESSVGGYLRTTIERLFAQLQHESV
                     VATLVSLGGLMLTASALFRHLRLSFRAIWRQAPPLVAGPIRVVVRATVLEYAVAYLMV
                     LAGGLLLVATLVLLSVTQWLGDLLIRVPLLSRTPAWMLALPGSVIVVGITFLLLFKFL
                     PPVRLRWRHVWLPAALCTAAWIAGAELMVFVGAVFGQSPTASGAFGGLLAAILWINAV
                     SQLLFLGAEMCKVIAWRDEAWNWPLKNAD"
     gene            complement(14289..15335)
                     /locus_tag="PKK95_04180"
     CDS             complement(14289..15335)
                     /locus_tag="PKK95_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOC17440.1"
                     /translation="MSLRALTLTLVMAASPGTLHAQPGDAVEVASGDRITGSVSRLER
                     GRLAFRTASAGPGHQRFAGTISIVWAEVVSLTCSQHLDVELASGERFAGSISSPSPGR
                     LVVQTASGPTRPIPVAEFVWIAPIEAGFRGRTVGSVDLGVSYSSAEGARTYTLRTEAS
                     YRSPGHAYEAQFAFDSWLSARDETETLTRNDFLFDVRRRLQGRWFAVAKFETQQDGPL
                     QLDVRILAGGGVGRVLVQSNRTRLYLQGGLDYDAERYESAQSFEHSAEVFAGVDWDWF
                     ESGGAVEAAVAATTFIGLERPRARLELDATIRRDVFWRLYWALSVHEGFDSDPPADRP
                     RSNLGLALSLGWSF"
BASE COUNT         2380 a         5687 c         5536 g         2317 t
ORIGIN      
        1 cccccgatcg cttctgtcgt gtcgtacgcc cgtctcccag cttaagggga attggcgcgc
       61 gcgtcaccgc cgatcgagcg acctcgagga tgagctggcg gcgccgcacc cgttactcgg
      121 cgcgcagcgc ctcgaccggg tcgatcctgg tcgcgcgcct ggcgggcacc caggcggccg
      181 tcaggcccac cgcgacgagc gccgcggtga ccaccgtgaa cgcgagtgga tcggtcggcg
      241 tcaccccgaa gaggaacgtc cgcagcacgc gcgtcacggc gatcgcaccc gcgaggccga
      301 gcaccgcgcc ggcggtgacg acctgcaggg cgcggccgag caccaggcgc aggacgtcgc
      361 tcctctccgc cccgagcgcc accctcacgc cgatctcccg gcgccgctgc gtgaccgcgt
      421 aggccatcag gccgtagatg ccgcaggtcg cgatcagcgt agcagccaac gcgaagagcc
      481 cgagcaccag catccggaac cgcggcggcg ccaccgacgc cgccaccgcc tcgtccatcg
      541 tcaccgtggc cgtgatcgcc aggtcgggcg cggcgcgccg gatctcggcg cggatgcctt
      601 ccaccacggc cgcggagttg gaggtgcgag cgacaaacga cacaaaccgc acgaagtcgc
      661 tgcggtcctg ctggaacggc acgtagatca tcggctgcac gtcccgatcg agccccatgt
      721 ggcggatgtc ggccaccacg ccgaccaccg tcaaccactc gctcccgtcg ctcagcccgt
      781 ccaggcgaac gcgccggccg agcgcctcgc ccggcccgcc cggcaagcac tcgcgcacga
      841 tgcgctcgct gacgatcgcc accctcggcg cctcggccgt gtcccccgcc gtgaactcgc
      901 gtccggcgag cagcggaatc cccagcgtct ggaagtagcc cgcgccgatc ttcggcctgc
      961 cggcagcgag gttcggcttc ggctgcccct cgatgctgaa gtcgccctgg atgaacatcc
     1021 tgccgaacgg cggcgagttg accaggctcg ccgccgtcac gcccggcaga tatcggacgc
     1081 ggtcgagcac gccgcgcgcg aaggcgtgga tccgctcggg ccggtcgaag agacgctcgg
     1141 ggagagtcac ccgcacggtc gagaccacgc cgtcgcgccc gctcgcttcg aacccgggcg
     1201 acaccgaggt gaggcgcacg aagctgtgca tcagcaggcc cgccccgatc aggaggacca
     1261 gcgtcatcgc gacctgcgcg acgaccagca gcgtccgcag ccgctcgccc ggcccgtcgc
     1321 ccgtcgtgcg cgcttcgccg gcgtgcagcg cccggccgtc ggcaagcgcg gtcccgctga
     1381 cgcgcagcgc cggcacgagg ccgatcagca ggcccgagac gagcgtcagg gcgatcgaga
     1441 acgccatcac gcccccgtcg atctcgatgc cgccgaggcg cgggagcacc gcctgcggca
     1501 ccagcggcag cgtgacgtcg atcgcgatcc gcgcgagcag cacgccgaac gccgcgccgc
     1561 cgagcgacag cacgagcgcc tcggtcagga gctgccgcac cactcgccgc cgtcccgcgc
     1621 cgagcgccat gcggaccgac agctcgccgc gccgcgccgt caggcgcgcc atctgcaggt
     1681 tcgcgacgtt gacgcaggca atcagcagca cgaacgcgac cgcaccgagc acgatcagca
     1741 gcagcgtgcg caccggcccc gcgccgtact cctgcaggcc ggccgtgcgc gcgatcgtcc
     1801 ggacgttcgg cgccagcacg ccttccggcg cgagtgcgcc cggggccacg atcgacgggt
     1861 cgttcaccag cgccgtcaac tccgtttcgg ctcgctcctt gttcacgccc ggcttcatgc
     1921 ggcccagcag gtagaggaag gcgcttctgc cggcatcgcc gggccagcgg ctgggcagga
     1981 agacgtctgc ctcgggcagc gcgccggcgc gaaccaccgg cctgaaactg aacccgcgcg
     2041 gcagcacgcc gacgacggta cggggcaggc cgttgatggt gatggactgc ccgacgaacg
     2101 ccggatcggc cttcaacgac gagcgccaca gcctgtcgct gacgatgacg gcgcgatcgt
     2161 cgtctgccgc cccggcgccc gctaggaacg tacggccgtg ggcggggccg acacccagcg
     2221 tggtgaagaa gttggggctc acttccaccg ccggcacctg cagtggctcc ccgcgcccgg
     2281 tgagctgcgc ctcgatcgaa acgtaggctg cgaggtggtc gaaggcgtcg aggcgcgggc
     2341 caagcgccgc gtacgacacc ggcgcccagg tccagatcgt cttgacgccc ttcccggtct
     2401 gctgcgcggc cgagaggatg acgagacggt cggggttggg atacggcagc ggacgccaga
     2461 gcacgccgta ggcgaccgag aagatcgcgg tgctgaggcc gatgccgagc gcgagcgtgg
     2521 caagcgcggt gatggtgaac gccgggtcgc gccgcatcag ccgcagcgcg tggcgcacgt
     2581 cctgagcgag cgtctcgatc gtcgggaacg tccacggctc gcgcgcccgc tcgcgcagcc
     2641 gcagcgggtt gccgacccgc cgacgggcgg cagcgcgcgc ctcgtcgccg cccatgtcgt
     2701 cggcgagcga ttgcgccgtc atctcctcgt gaaagcgcag ctcctcgtcc aggtcgcggt
     2761 cgagccggtc gcggcggaac aggaacagca ggcgtctgag cagagaaccc acggcagcaa
     2821 ccccctggcg agccggcccg tcacgcggcc attacgtcgt cttcatcacg cgcatgatcg
     2881 cgccgaacat ctgcgagaac ttcgactcct cgacgccgag ctgcttcctg cccgcggggg
     2941 tgagcgtgta gaaccgcgcc cgccggttgt tggccgagac cttccactcg gccgataccc
     3001 agcccttgac gagcatccgc tgcagcgcgg gatacaaaga tccctcctcg acggtgagca
     3061 cggcccgcga gatcacccgg atgtgctgcg cgatgccgta gccgtgcatc ggctcgatcg
     3121 acagcgtctt gaggatcagc atctcgagcg tgccgggcag aagctcgttc ttgtccttcg
     3181 tcgcgttcat aagtccgcct ttgcatagac cgactatgga agcagatggg agtgtgctct
     3241 atcggggaga tcgtgtcaag ttcaggcagg cgttggcggt tcatcggcga agctcagcgg
     3301 caggcgccgg ccgccgctcc gcctcgtcgc gagcgatcga attgcggagg atgtgctcag
     3361 gttggtctac gttccggctt gaccctgtca gttctagaac ctctgcgtcc tccgcgcctc
     3421 tgcggtgagc cgttcgtaag gccagggcgg cgttttcgcg tcctctgcgt ggccgacctt
     3481 tcgagctgaa gcgtcctgct acctgctcgc ggcactgtgc gccggatcgc cgtgacggag
     3541 gcccgaaccc gccaggacaa cggcggacgg gtcgtgcgcg tacgaaccaa cctcgaggtc
     3601 cacgtccgcg gggtctggca tcacacgcgt cttcttcgac gcgtcccacc gcgccagcgg
     3661 tgtgaacgac acgggcacct cgacgcggac cgacttcccg gccgccacgg gggcagccgc
     3721 gaaaccgatc agcatcagct cgccgggata gccgccctgt cggcagcgcc cgtacacctg
     3781 gacgacgtgg tgagcgtcgc gccggccggt gttcgtgaca gtggcggcca gccgcatgcc
     3841 cgccgggccc cgttcgatca cggccacctc gccaattccg aacgtggtgt acgacaggcc
     3901 gaacccgtgc gggaacgccg ccttgacgcc aagcttgtcg agcaggcgct ggccgtggaa
     3961 gcggtcgtag gtgaccttgg tggccgtgcg atcgaacggg ggcaggtggt cggaggacac
     4021 cggaatcgag aacggcagac gccccgacgg gttgtggacg ccggtcagga cgtcggcgag
     4081 cgcgcgtccg ccctccatgc ccgcgtacca catcatgagg atggccggaa cctcgcggcg
     4141 ccaggcctcc atcatcacgg cgcccgcggc gacgatgcac acgatcgtgc gggggttgac
     4201 ggcggcgacg gcgcggatga tctcctcgtc ctgggcacga agcgcaagcg aggcccggtc
     4261 gcccccgaac tggtcgccgg cggtcatgac cgggttcggg ccaggccccg ccggccggct
     4321 tccgccccct gcggcagcct gcggcctgcc cccggagggc ggcagcggcg ggaagagcgc
     4381 cagcagctcg gggcgccgca tcgtgtccgc cccgacgtac tcgccctcgt cgcgcgcgtc
     4441 gtacccggcg atgacgatgg ccacctccgc cgagcgggcg atggcacgcg ccgccgccgc
     4501 gtcgccgtcg gtgatggccg cgatcttcgc gccggggaac gccgcggtga ttcccgcgag
     4561 cggcgagacg tagcccggcg cccggacgtc ggaggaaccg tggtcgccca tgttcggcgc
     4621 gtccgcgagg cgcccgacga cggcgatcga cgagacagcc gccggcgtca gcggcagcag
     4681 tggcgcgccg ccgacctcgt cgttcttcag gagcaccatg gaacgcgccg cgaccgtccg
     4741 ggagagcgcg cgtgcctcct cgttcgccat gaggtcgagg ccgaacggcc cctgggcgcg
     4801 ggtggcgtac gaccgcagct gcgcggccag cagccggacg cccgcgcgct cgaccttgtc
     4861 ccaggaggtt tcgcccgccg cgagctggcg gcgcaggtga gtggcgcgct gctgggcgag
     4921 gggctcctcg aggtcgaggc cggcgttgag cgacttggcc gcgtcgcgca ggccccagat
     4981 gaagtcgctg acggtgatcc cgctccaccg ccactggttc cgcagcacct ccgtcaacag
     5041 gtactcgttc tggccggccc actcgccgtt gacggagttg tacgccgaca tgacggccgc
     5101 gacaccctcg tccacggccc gcttgaagtg cggcaggtac acgtcgtgca gcgtcgcctc
     5161 gtcgatggtc acgtccaccg tgaaccgggc gttctccatc gagttcaggg cgtagtgctt
     5221 cgcgcaggcc atcacgtact tctgcgcgcc ccgcgtcagc gcggcgccga actcgccgag
     5281 gtggcagggg tcgtcgccgt aggtctcctg gatccggccc cacgccggat ggcgcgggag
     5341 gttgatgcag acgccgccga agaagttgcc gccctgtgcc cggatctcgc gcccgatcac
     5401 ctcgccgacc ctctcttcga ggccgacgtc ccacgtcgcg ccgcgcgcca tcgacacggg
     5461 gaatgccgtg ccctttccgg ccacgcagcc gcgcgggccg tcgacgaacc gcgtgcctgg
     5521 gatgccgagc cggccgatgg cgccgtgcac gtacggccgc gcgttgtagc cttcctcccg
     5581 catcgaccgc atgccctccc agaagggcgt gtcgccgtcg agcaggttga gccgctcgtc
     5641 ctcggtgagc cgatcgtaga gcgccttcgc ctggccctcg ggcgcctcac ccgcccgcac
     5701 ggcggcgacg gcgcggtcga atgcagtgtc agatccctca cgagcggcga tgtgggtcat
     5761 ggggtcctcc agagaccgat cggacccgca catcctctct cgttcgggcc gcggaggcaa
     5821 tattcggggc gtgcgccgcc ctcaccgccg tggcttcggc gggggccgcc gcctcttcac
     5881 cggcccgcag ccgccgctcg acggcctccc caccgctccg cgtactcccg gaagatctcc
     5941 gcttcggcga tgacggtctt cctgaactcg tcgagcagga cgcgctcgtt cggcgcgtgg
     6001 atgttgctga agccgtcggt cgccccgagc aggaggatct cggcctgcgg gacggcttcg
     6061 tgcagcgcgt tgacgagcgg gatcgaaccg ccggtggcga cgctgaccgt ctcaccgccc
     6121 caggccgacg agagggccgc acgcgccgcg tcgtatgccg gaccggaggt gctggcggca
     6181 aagccgttgc cgatggcgcc gggcgtcacg tcgagcgcga tcccgaaggg acgcagtccg
     6241 cggagatgcc ggacgagtgc attctgcgcc tcagccgcgt tctgctcggg atgaacgcgc
     6301 aggttgatcc tggcgcgcgc ctgcggcacc acggcgttga ccgccttgtc gaccggcagc
     6361 gcatcgaggc ccgtcacggt aatcgccggt cccgaccaga gccgctcgcc cagggtgccg
     6421 gtgccgaaca gcggaaggcc gggcatgatc tctgcgagcg cgcggaactc ccgctcgtcg
     6481 tacgacgcgc cggtccacgc gtcgcggcgc aggccctcga ccgtaacgtc gcccttcacg
     6541 tcgtgcaggg tcgcgagacc gtgcaggagc gcaatcagcg catccggcgc cgcgccgccg
     6601 tattgcccgc tgtgcttcgg gccggcgagc gtccggacct cgaccgtcac gaccgcggtg
     6661 ccccgaaggg caacggtcag cgtgggcacc ccgggcctga cgctgcccat gtcggcgatc
     6721 accatcgcgt cgcagcggaa ccggtcggcg tcgaggggcg gatacgtgtt gaaggcgctg
     6781 cccacctcct cctggccctc gatcacgacc ttgattccaa ccggcggctt cccgccgtgc
     6841 gcgcgcaacg cgcccacgtg cgcgaggacg ttcgccttcg agtcggaggt gccgcggccg
     6901 aacagcgccc cgtcgcgctc ggtgggctcg aatggcggcg acagccactt cgactcgtcc
     6961 ccgggcggca cgacgtcgta gtgcccgtag agcaggacgg tcggcgcgcc ggggggcgcc
     7021 ggtatctccc cggtgacgat cggatacgtg ttcggcagat ccagctgcga cacgttcacc
     7081 ccggcatcgc gcaagagctc ggagaccacc agatgcgcct cgtgcatcgg ctcgggtgga
     7141 aaccccgcct cggaaatcga aggaatgcgt gagagccgga cgagttccgc cgtcagctgc
     7201 ggcatcagcg aatcgacggc cgcggccatc ggctcgcgcg acgcgccgga tcgttcgtct
     7261 gtcatacggg ccatagggaa cctcctgggg ctgcgtcctg gcaggagcca actccgtgcc
     7321 acgccagcgt ccgcgcttgc ccggctggga cggcctgacg agtcgcggca cccgccgcat
     7381 gtcgcgagcc ccccacgtaa tggcgccgat tgcccgtccg catccagccg tcgcccggct
     7441 ttcacgttcg cgcgcgggcc tgcacgcacg catgccaacg tgcgggatct tgcagaacgg
     7501 aacggccgcg agccgacttc ctccagctcg aggccgccgc gcccggaccc gatcaggagc
     7561 gttccatgcc tgtgcccgcg cgagacacca accgtccggc ggccgccgcg ccgttcgttt
     7621 ccaccggccg cctgcctccg cccgagcggg tccgcagctg ggttctcgag gcacacgaga
     7681 agtaccgcct gaacggcgaa gggcaggtgt cgcaggtcta cccggcgctg gcgcgcgtgc
     7741 gccccgacct cttcggggtg tgcgtcgtcg cggccagcgg cggcgtctac gccgccggcg
     7801 atgccgggca cgagttcgcg atcatgagcg tgtccaagcc cttcgtgctc gccctggtct
     7861 gcgaggctct cggccccggc gaggtgcgcg agaaggtagg tgcgaacgcc acgggtctgc
     7921 cgttcaactc gctcgcggcc gtcgaagaga gccccgacgg caggacgaac ccgatggtga
     7981 acgccggggc catcgcgacc acgagcctgg tccccggcgc gacgccggag gcacggtggg
     8041 agtttatcca cgaagggctg tcccgcttcg ccggccgagt gctgacgctc ggagaggagg
     8101 tgtacgcctc ggcctcgacg acgaaccaga ggaaccaggc cctggccctc ttgcttcaga
     8161 gccgggatcg gatctactcg cctccctcag aggcgacaga cctctacacg cgtcagtgct
     8221 cgctgaacgt gaccgcgaag gacctcgcgg tcatgggggc gaccctggcc gacggcggcg
     8281 tgaacccgct gaccgggcag acggtcgtcg gcacggcgac ctgtcactac accctggccg
     8341 ccatgagcac cgccgggctc tacgaaacct ccggcgagtg gctctacgac atcgggctgc
     8401 ctggcaagag cggcatcggc ggcggcatcg tggccgtctc tccgggcaag ggtgggctgg
     8461 gaaccttcgc gccgccgctc gatggggcgg gcaacagcgt gaaggggcag ctcgtggcca
     8521 ggtttctatc gcatcagctc ggcctggacc tcttcgcgtc gcggcccgcc gattcatccg
     8581 acccgcgggc gtgagccggg caacgcctcg gggggctcga cacgacctgc cgccctgggg
     8641 ccggggccgg ccggatgatc cccactccgt tgccgcgaga tgtcgcggga aagccgcacg
     8701 ccagcgtgag tcccgcctga ttcgagtggt cgcgttcagc acggcacaac cgtggcaacg
     8761 ctcatgcagt ccgggtcagc ggcacaacac gccgtcgtag gcggcgatca agtgggagcc
     8821 ggctgttggc ggatgcgaac tactggggcg ctttcctctc ggcgctgctg ccgacgctgc
     8881 tcatcgcgtg gctggcagcc tggctcgcca ggcgcctggc tcggaacgcc actctcgccg
     8941 ttgttggtga caggctccgg gctgacagcc cggtgatccg cggaccgctg cggctggttg
     9001 ccgctgcggc gttcgtgctg gtcgtggcgt tgctcctctt ccccgcgatg gagctggccg
     9061 gctttcgccc gaccgtcggg cgcaaggcgc gcgagatggc ggtctggctc ttcggcggcg
     9121 ggttgcgggt gggcatgatc gccctgattg cgtacagtct gcgccgcgcc acccttctgt
     9181 acgtgcagcg cttcgaagac gagctcaccg cgggcgccac catcggcgaa ctcgaacgga
     9241 acaggcgcgc ccgcacgctc ggatcgatgg tcaacaaggc catcaccggc ctcatcgtcg
     9301 gggtcgccgg ggtcctgatc ctggacgaag tcggcgtcga cgtcgccccg atcctgaccg
     9361 gggcggggat cgccggcatc gccatcggct tcggggcgca gacgctcgtc cgcgacgtcc
     9421 tcagcgggtt ctttctcatc ctggaagacc aggtgcgcgt gggcgacgtc gccgagatca
     9481 acgggacgac cggcctcgtc gaacaactca acgtgcggac gatcgtgctg cgcgacgaca
     9541 ggggcaccgt ccacgtggtc cccaacggcg ccatcgtcac gctggccaat cacagcaagg
     9601 acttctcccg ttacgtcatc gacgtggatc tcccctacgc agaggatccg gatcgcgtgg
     9661 ccgagatcgc gcgcgatgtc gatcgggaaa tgcgcagcga tccgaagttc aagattctca
     9721 tcctcgaacc tatcgagatc gtgggcgttg tcgcgtacag ccagtggtcg atgcagtgga
     9781 ggattcgaat gaagacggtc gcccagcggc agtgggtggt ggggcgggag ttccgcaagc
     9841 gcctcaggaa ggcgttgaac ctccacggga tcgaggtgcc gtatcccgtg ttccgcccct
     9901 gacgcgcggc gcgccgccgc aaggctgcgt ggcttccggc ttgcaccagg cgcccgcagc
     9961 ccgtcgttaa ccccggtgcc cgcatcgggc acgcacactg gccggaggct ccgatgccca
    10021 gagagctggc ggaaaaactg aaggactcga tcgtatcgac cgtacggggg accggagaga
    10081 tcgtcaacgc cgtcaccgag accgtgagcg acaccctcgt gacggcgctg caagggaccg
    10141 gatcggtcgg caaggcgctg actggcgccg tcgccgacgt ggccagcggc gcgatcgaag
    10201 gaacgtcgca ggtcggcggc gacctcggca aggccgccaa gggcgccgtc atcggcgttc
    10261 tgcaggggac caagcgggtc ggcgccgagg ccgttgacac gatcggctct acggtgcaga
    10321 cgctggtgaa gacgaccgtg gaggtgggcg gcgacgccgg ttctgccgcc cggcaagcga
    10381 tggaaggcac catcgagggc gctcgcagca tcaagctggc gggggtcgag gcggtcacgg
    10441 cggcggccgc gggcgccgtc cagggcgtcg gacgcgtggg cgtctcggcc acgacgacga
    10501 cggccgacgt ggcccgggaa ctgatcagcg gcgcggcgaa cgtgggcggc gacctgggct
    10561 cggcggccaa gggcacgatg ctcggcgtcc ttcgcggaac gaaggaattg acggcacagg
    10621 cgaccgacac ggtcggcgcc acggcaggca gtctcgtcag ggccacggcc ggggtcggcg
    10681 gcgatatcgc cgcgactgcc agggctgcgg tcgacggtac catccagggt gcaagggaag
    10741 tcggtgtcga tgcggctggt gcagcgtcgg ccgccgcgac cggcgcgctc gcggccgccg
    10801 gcgacatcgg caccgccgcc gtcgagcagg tggaacgggc cgcgaccggt gtgatctccg
    10861 gcgtgaaagt ggtggtcacc tcgccgttcc gagggtagga acggcggcgc cggcgcctac
    10921 gcgggcgagg gcggccgcgt gcgggtgccg gaggcgggct tcctgcggcg acgcggcttc
    10981 atgcgccggc ggcggacgct gctccggacg gccccctccg tcgcctgcag ggcggcgtcg
    11041 atcgcggcgc ccagcgagcg gtgtcgccgc tcgaccacaa cgcttgggag cccgctgagg
    11101 accaccttca cgcggcacac ctggtcgacc ccgccgcgcg gcccgttcgc gtcggtcacg
    11161 cggacgctca cgcgctcgat cgacgtcgca aacttcccca acttcgcgcc cagcttgaga
    11221 cggatgagct ttcggtcgtc cttgtcgagt tccactccga tcacacgcac gtgcgcgggt
    11281 gtccgcgacg cgtccgtgcg gccgctcgct cgcttcacac gtttcggaat gaaccggggc
    11341 attgcgggct gagtcgccat cctgctactc cttcgagtcg cggcccgcct gtcgccgccg
    11401 gccggctgac gacgtgtggg ccctcgaacg gtgggcatgc gaggcgcgca gcacccgcgc
    11461 cttcgcgctg gggcttgccg cctttcgtgc ccttcgcttt ccggctgcct gcgggccctt
    11521 cccgcccgct gcgccagtgc tgccggatgc ggtgcgccgt ttcgccgcct tcctctggac
    11581 cggtcgcttg tcattcttca tcggacttgt cctacccgcg acatatcgcg aaggccgcca
    11641 cgaggtggcg cctgatgtac gccggcctct tcctgccgcc cccattcagc cggcgcgcgg
    11701 gggcgagccg gccggcaacc ctgccccggc ggtggcccgc cgcatgcccc acatggtgag
    11761 cgcgatggtc gccgtcgcgg tgacgatggt gaagagcggc aggccccacg acgcgatgag
    11821 cgggaccgac agcgcggtga tgaacccgcc gccgagcacg gggcgggtga tcaactgccg
    11881 gtagctgtag gctctcacca cgtcggtcct gcggtccggg tcggccatgt cgaccatcac
    11941 gaagcccgtc gccacgttgc cctgcgactc gccgaactcg gccagcccgt gctcgaacca
    12001 gttgcgctga aagatccgcg gcgagatcac gagcgtgacg aagacgctcc acgccaccga
    12061 cgccacggcc agcacgatca acggcccgag ctgggagccg agcgccgcga gcgacagcgt
    12121 gccgatggcg cagatcacga tgccgtccac ggcaagcccc ccgagcccct cgaccgcgag
    12181 gcggttgacc gcccactcga agttgaacct caccgcgcag acctgaacga tcgcgccgcc
    12241 caggatggtg aaggggaaga gcggaaactt gtcgaagaag ctcgagccga gcagggagaa
    12301 tgtcacgcgg aagagctcga gcaggacgta gccgacggca atcgacacgc cgatgaacac
    12361 ggccgccgcc gtgacctggg tcatgccgcg ccactcgtca atggggacgt cgtcggggcc
    12421 gggcaggtgg tagtcgatgt cgtagtcttc ctcgggagag gtcggctgct gacgggcgac
    12481 cgggattcgc ggcgaggtga tcgcgtagtt gacgagcgcc gtcccgatca cgatcccggt
    12541 gaccatgccg atggtcgcca gtcccagtcc cagctcgagc agttcctccg cgccgtattg
    12601 gacgaggacc ggggtcaggc cggcgagcgt gccatgaccg ccggcaaacg acatctcgat
    12661 gatcgagccc gccttgctgt tgaggccgaa gagcggcccg agcaccagca gcaccaggat
    12721 gctcccgacc gcgaactgac ccgccgacat gatggaggcc aggaccacgt ggctgcgcga
    12781 gatcgtccac acggtcttga gaggcggcag gtgctcgccg agcaggagcg cggcgcacat
    12841 cacgttgatc agcagccccg gcagggcctt ccacaccgcg agtgtctcgg ccgagaacgc
    12901 accctggccg ccggtgacgc ggcccacccc gtccggcccg agcgcgagaa cgaggaagcc
    12961 gccgatgacg gccgtcggaa tgaaccgtgc gcgcaacggg cgtgaccagc gccggatgag
    13021 cgccgcgaac gcgaacatga gcgcgaggat caccaaggcc ggagccatgg cttccgcggt
    13081 gaacggcacg gggcagctcc atgcggaagg gcgcaaccaa cggtcgtgat accgcgtgat
    13141 accggcgtcg cggatgcgac ctgcacggac caggccagag gggctcgcct gccgcgaggc
    13201 gatcggcacg cggctggcat ctcgggttca cgggaaaggc ccggcttgca gccgcctacg
    13261 ctacggtcac gaaccacgcc gaactcgttc gccggcctgc tgcgggcagc ctggcaggag
    13321 tacgagcgcg accacgcgcg gtacttcgcc agcgcgatgg tctaccatgc gctggtctcc
    13381 ctcctgccgc tgctgctgct cgtcctggcg gccctcgggt tgttcctgcg cttctcgacc
    13441 ttggcagcgg gcgtcgaaga gcaggtcctc caagccgtcg aaagcagcgt cggagggtac
    13501 ctccggacga cgatcgagcg gctcttcgcc cagttgcagc acgagtcggt cgttgctacc
    13561 ctcgtcagcc tgggcggcct gatgctcacc gcctcggcgc tgttccgcca cctgcgcctg
    13621 agctttcgcg cgatctggag gcaggcaccg ccactggtgg cgggacccat ccgtgtcgtc
    13681 gtgcgcgcaa cggtcctcga gtacgcggtc gcctacctga tggtcctggc gggaggcctg
    13741 ttgctggtcg cgactctcgt cctgctctcc gtgacgcagt ggctgggcga cctgctgatc
    13801 cgggtgccgc tcctcagccg cacgccggcc tggatgctcg cccttccggg ctccgtcatc
    13861 gtggtcggga tcacgttcct gctgctcttc aagttcctcc cgccggtcag gctccggtgg
    13921 cgccacgtct ggcttccggc cgcgttgtgt accgccgcct ggattgcggg ggccgagctg
    13981 atggtgttcg tcggcgccgt cttcgggcag agccccaccg cctcgggagc cttcgggggg
    14041 ttactcgccg cgatcctgtg gatcaacgcg gtgagccagc tgctcttcct cggcgccgag
    14101 atgtgcaagg tcatcgcctg gcgggacgaa gcctggaact ggccgctgaa gaacgccgac
    14161 tgacggcact tccggcacca ccagcactgc accttcagca cctcctgcac cgtcggcacc
    14221 tcctgcacga tcattcgaaa gagatctctg cgtcctcggc gtctccgcgg tgaaatgtcc
    14281 gcaggacatc agaacgacca acccagcgac aacgccaggc cgaggttgct gcgcgggcga
    14341 tcggcgggcg ggtcgctgtc gaacccttcg tgcacgctca gagcccaata caatctccag
    14401 aacacgtcgc ggcgtatcgt ggcgtccagc tcgagtctcg cccgcggccg ctccaggccg
    14461 atgaaggtcg tcgccgcgac ggccgcttcc acggcaccgc ccgattcgaa ccagtcccag
    14521 tccacgcccg cgaagacctc ggcagagtgc tcgaaggact gtgcactttc gtaccgttcc
    14581 gcgtcgtagt ccagtcctcc ttgcaggtac agccgcgtgc ggttcgactg taccagcacg
    14641 cgtccgacgc cgccgcctgc cagtatccgc acgtcgagct gcagcggtcc atcctgctgc
    14701 gtttcgaact tcgccaccgc gaaccagcgg ccctggagcc tgcggcgcac gtcgaacagg
    14761 aagtcgttgc gtgtcaacgt ctccgtctcg tcgcgcgccg acagccagga gtcgaaggcg
    14821 aactgcgcct cgtaggcatg ccccgggctg cgataggagg cctcggttcg cagcgtgtac
    14881 gtccgcgctc cctccgcgct cgaatagctc acgccgaggt cgaccgaccc gaccgtgcgg
    14941 ccgcgaaacc cggcctcgat cggcgcgatc cagacgaact cggcgaccgg aatcggcctg
    15001 gtcggtcctg aggcggtttg caccacgagc cggcccggcg agggcgaaga gatcgatccg
    15061 gcaaacctct caccggaagc aagctcgacg tcgaggtgct gcgagcacgt gaggctgacc
    15121 acctcggccc agacgatcga gatcgtgccg gcgaatcgct gatgcccggg accggctgac
    15181 gccgtgcgaa acgccagccg gccgcgttcg agccggctca cgctgcccgt gatccgatcg
    15241 ccgctggcaa cctccaccgc gtcgccaggc tgtgcgtgca gcgtccccgg cgaggcggcc
    15301 atcacgaggg tcagcgtcag ggctctcagt gacatccgcg gcatcacgta tccaggggca
    15361 tttggctggc cgcgtgcccc ggccttcagg ccaaggcggc cgtagcgctg cggccgtagc
    15421 ccagggcttt gccctgcggc gtcgcgcggg gcctgagctc tgcggccgct cgactgccag
    15481 cgacgtgcca cccagcggtc gcggttcccg gcagcgccgg gcagtcctcg ctcgttccgc
    15541 acgcgtctcc ggtgcgccga gaggcgccga tgaatgggcg aggcacacag cccgagtccg
    15601 ggcggggccg cgaccccgcg cgggacgctc gccgcgggac gcgggcgggc gcgcccgtgc
    15661 gtagcgcttg ttgtagcgcg gggctttggc cctgcggggc tcaggcttta gcctgcgggg
    15721 ctcaggcttc aaccctgcgg gctcggcgct cgagggccag gcttcaaggc tcgaggctcg
    15781 cggttggcag cgttcgtgta gacgagggtc acgcggatcg ggcgagcgaa ccaccgcagc
    15841 cttggcgcgg gatggcgagc gagatggcaa agcacagcga tcgcgctgtg ggcgcgggtc
    15901 aagccctggc atcggattcc
//
LOCUS       DAOPQX010000046        15856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4400_length_15856_cov_5.632618, whole genome shotgun sequence.
ACCESSION   DAOPQX010000046 DAOPQX010000000
VERSION     DAOPQX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15856
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4400_length_15856_cov_5.632618"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1819)
                     /locus_tag="PKK95_04185"
     CDS             complement(227..1819)
                     /locus_tag="PKK95_04185"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC17441.1"
                     /translation="MLSGEARYRLLLQVAEAANSSLDMTGVLEEVGRVLEPLVALDAI
                     AIVTVDGAELRPHAIHIRGVAARQGETVHGSIARALDLSREEYEARYGRPIALAGSGT
                     ELVGRTGRAEVQQDLAAARRYTEDERLLSYGVRAYVRVPLVFRGRLVGSLTFARLSSS
                     PFSPDEAALLEELARPIAGAVSNALAYEEIGRLKNRLHEENLVLRQELDEQSMLEEIV
                     GSSPPLRQLVREIERVAPTDTTVLVTGETGTGKELVARAIHRHSPRAGRALIKVNLAS
                     MPDTLVASELFGHEKGAFTGALQRRIGRFELASEGTIFLDEIGELPGDVQVALLRVLQ
                     EGEFERVGGNQTIRTRARVVAATNRDLPAAVAEGRFRSDLFFRLNVFPLFVPPLRDRR
                     EDIPILVEYFLARHGSRLGRRFRGVDRATMALLRSYAWPGNIRELQNVIERAVILSDS
                     EILHLDETLLTAGVGSLASRAARPAGTLHDEEVRLIEGALEACGGRVAGPGGAAARLG
                     IPPTTLESKIKRFGIQKNRFRR"
     gene            2010..3386
                     /locus_tag="PKK95_04190"
     CDS             2010..3386
                     /locus_tag="PKK95_04190"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOC17442.1"
                     /translation="MEPSVIDFINTSRDRYLEELKQYLAIPSVSALPEHAGDLRRCAE
                     WTAEQMRAIGLENVRVADTPGHPIVYGDWLHAEGAPTALVYGHYDVQPVDPVELWTSP
                     PFEATVRDDELFARGATDDKGQLFAHLKAIEAHLKQAGRLPINLRIILEGEEEIGSPN
                     LNQFILDHKHELACDVVLISDSPMFEKGVPSICYGLRGLAYCQIDLRGTSTDLHSGSF
                     GGAVANPAFVLAQVLSQMKDRGGRVKIPGFYDDVRELTEREREEFKRLPFNEKEYRKG
                     IGAPKLHGETGYSTLERTWARPTFEVNGLLSGFTGEGSKTVIPATAMAKVSMRLVPNQ
                     DPDKIAALFEDYVRKVTPKTVELKVTRMHGGKPWMTELDNPFVQAAARAIEVGFNRTP
                     VYTREGGSIPIVATFQAELNAPTVLFGLGTPDENAHAPNEKLYLPNFYGGILSAAYLY
                     EEIARARQ"
     gene            complement(3851..4432)
                     /locus_tag="PKK95_04195"
     CDS             complement(3851..4432)
                     /locus_tag="PKK95_04195"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HOC17443.1"
                     /translation="MLAAAVFVAACSGGAPDEAKYLADLARERAAKDAELAEAANSPV
                     KPEDRGRFLPLAYYQPDPRYAVPASFHPEPPAQRTRVVIQTSADKPRLMERLGTLRFT
                     LDGQRLALTAFVEAGQPADRLFVPFADHTSGKETYAAGRYLDLVPQRTGIYVVDFNRA
                     YNPYCYYNASYDCPFPPKENRLPLAIRAGEKTK"
     gene            complement(4471..5130)
                     /locus_tag="PKK95_04200"
     CDS             complement(4471..5130)
                     /locus_tag="PKK95_04200"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC17444.1"
                     /translation="MTHHIPGPAGRLEALVDDPASLCTTAMPTPEQAAAAPPKPRAVV
                     VIAHPHPVYGGTMNTKAVFQAAKAFCRLGCAVVRFNFRGAGRSDGTFSNGPGEIDDYR
                     AALDFAAARHPGVQIWSAGISFGAWVATTAGADDARVTRLLAVAPPVDSYDFGPLARS
                     PKPKFFIQGERDEICPTPALWQFYAHAAEPKDIAVIDAANHLFDGRLTEMSEAIERFM
                     E"
     gene            complement(5154..5951)
                     /locus_tag="PKK95_04205"
     CDS             complement(5154..5951)
                     /locus_tag="PKK95_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC17445.1"
                     /translation="MFLCGSGPPLVLIPGIQGRWEWMRPAVHALAASFTVATYSLVGE
                     PDSNAPLRPDTPFDRHLEQVDDVLRQNGWSQAVVCGVSYGGLVAVRYAAVRPERTRAL
                     VLVSAPGPRWRPAARVRRYVSSPWLSAPAFVAGAPLRLWPEIRAAAPSRLQACTCGAR
                     HLVNVALAPAWPSRMANRVRCLEGLDFRVDAGRVRSPTLVITGQPHLDRVVPVDETLE
                     YVSTIPGASARTLAGTGHLGLVTRPREFAGLIADFAGAGALVAQTHS"
     gene            complement(6001..7065)
                     /locus_tag="PKK95_04210"
     CDS             complement(6001..7065)
                     /locus_tag="PKK95_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOC17446.1"
                     /translation="MTTSLAAASAVAALPLMLAMTQTLAPRPSPQGPEGLTAVAGLKV
                     GHHTLRERPTGCTVVLAEAGAVAGVDVRGSAPGTRETDLLEPVNLVEEVHAVVLSGGS
                     AFGLDAASGVMRFLDERGIGYQTAVAKVPIVPAAILFDLGIGSPKIRPTADCGYRAAQ
                     AATSGPVAEGNVGAGAGATVGKMGGQHRAMKGGVGTAALVLPDGLVVAALVAVNAAGD
                     VVDPATGQVVAGVRTEDGRRLADVRTLLRGGSLVPRAKPGENTTIGVVATNARLTKTQ
                     TTKVAQMAQDGIARAVVPAHTPFDGDTLFALATGTHEKPADLLTVGSLAAEAVAEAIL
                     RAVRAAKGLEGYPSASELAR"
     gene            7127..9574
                     /locus_tag="PKK95_04215"
     CDS             7127..9574
                     /locus_tag="PKK95_04215"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOC17447.1"
                     /translation="MIIRRFTPAAAAALLLVSAQVYSQSQSQSGAQAVGAGQVAPARA
                     AVSGLDPARMARIAELVNEAMEAKELPGAVVMAGRGDEILYQQAFGRRAVRPSSEPMT
                     LDTVFDLASLTKVVATAPAVMMLVEDGKVRLNERVSKFIEGFERYGKGDITVRHLLTH
                     VSGLRPDLDMALEFDSYDEAIERAKEEVPIARPGERFVYSDINFFLLGEIVRKVSGQG
                     LDQFVRERLYQPLGMLETTFKPGASLVSRIAPTEPCSPLGWPCDTPDARMLRGVVHDP
                     TARRMQGVAGHAGLFSTAADLSIFCRMLLNHGRYGQVRVLSPLAVEKMTGPVPVPGGV
                     RGLGWDIDTGYSSNRGELLPIGSYGHTGFTGTSIWIDPVTKTWLVFLSNRVHPDGKGD
                     VTPLRARIATIVGSAILEVPAGIAQPLRMTGIDPGPTGTRIPRVGPPVLNGVDVLKAE
                     NFRRLAGRRVGLVTNHTGITADGESTIDLLASAKTLSLVALFSPEHGIRGTLDQSVIK
                     SDRDQKTGLPIYSLYGETNRPTGEMLEGIDTLVYDIQDVGVRFYTYETTLAYVLEEAA
                     KRKIKVVVLDRVNPINGWMVEGPALEKETASFVGYFPGSPIRHGMTVGELALLFNAEK
                     EIRADLSVVPIAGWRRDLWFDDTNMPWVNPSPNMRNLIQATLYPGIGAIEYSNLSVGR
                     GTDTPFEQVGAPWIDGRRLAARLNARQIPGVTFYPTWFTPSSSKYKGERCGGVYIMIT
                     DREALRPVRVGVEMAAAIHGLYGDKLQLDPNVKLFGTGKVLTAIKKGEDPARIAASWA
                     ADEGRWRLLRNKYLVYR"
     gene            complement(9609..10502)
                     /locus_tag="PKK95_04220"
     CDS             complement(9609..10502)
                     /locus_tag="PKK95_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOC17448.1"
                     /translation="MRAAAALAASLVLGATLGAAEFEKILPAARGTSLDLRLFGGEIA
                     VRAWARDEVRIRATHFSTDEIDAAAQGQTITVRARARRGQPHAIDFTIDVPAWMPVAV
                     AGTYLDVSVEGTAAAVTARTVRGDVRVAGGAGEIVLASTEGEVTLEGGHGRASLESVN
                     SAVRANGFEGELVAESVSGTVKLRSVKSASVAASTVSGDIAWDGAVAAGGRYQLATHS
                     GDIDLALPDAASATLAVRSFEGHVHSVLPVKLPDLPARRKRFTLTLGNGAARIELETF
                     TGTVSLRQETGSGLETQGPRP"
     gene            complement(10511..11224)
                     /locus_tag="PKK95_04225"
     CDS             complement(10511..11224)
                     /locus_tag="PKK95_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOC17449.1"
                     /translation="MTREAMRAGESGDRWTGLLSDYLDGELSPGERRALEDHLPGCAQ
                     CRAALEELAAVVQRAPALRQDEQPAADLWPGIEQRLGPRRAPRARLGALVPAWPAAWP
                     RLWPAFAAAALVLAVAAGLVLPRAGRSVAPEQASVAEPQPPEPRGDLRPLEASREYYD
                     TLAQLQQAARARLARDPRVVEVIEQNLEVLDMAIARYTDALAEQPGDSRLASRLEAAR
                     QRKIDVLQQAVDLAAEAGN"
     gene            complement(11221..11736)
                     /locus_tag="PKK95_04230"
     CDS             complement(11221..11736)
                     /locus_tag="PKK95_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOC17450.1"
                     /translation="MALARQGDEGAFERLYRANVGRIHGLACRMAGYGAADELTQDVF
                     VRAWEKLDTFRGDSQFGTWLYRLAINAIVERRRSAARRSMIETHDEGMALDLKGVAPI
                     DGVAAMDLRAAVDRLPEGARQVFLLHDVEGYKHREIGKLLGIAAGTSKGQLHRARMML
                     RGALRGLDESS"
     gene            12010..13350
                     /locus_tag="PKK95_04235"
     CDS             12010..13350
                     /locus_tag="PKK95_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17451.1"
                     /translation="MTRSRAVFACLVLVALAGVLAQGQARSVQGEWTARVEDDGRVHL
                     QLHTDDLEPRQAGRQPSRPRGDWSFGRSFQLSELKGLPAQKGALTVENARFELPREAG
                     TIAFQGSFRDGRGAGLFSFTPRSEYVGEMKALGYAVEDTSASGLFRLAAFDVGRRFHD
                     ELASLGYTKLPLEMLVRMRTHGADGEYIKELQAAGYRDVPADQVVRARDHGVTRDYLE
                     GLAAAGYKGLPLEQVVRARDHGVTHDYLEGLAASGYKGLPLEQVVRARDHGVSAKYLE
                     ELSAAGFKGLPLDEVIRARDHGVTPEFSRELRQLGYSPSLDELRRLRDHGVTPGYVRE
                     MREAGYGDAPLEELIRLRDRGVTGSFVREMAQAGYSKVPLQSLLRMRDHGVTPGYIRD
                     LAGVGYKDIAPDLLVRMRDHGVTPSFIRRAQEQGHKNASPEELIRLRSRGIVRD"
     gene            complement(13436..13843)
                     /locus_tag="PKK95_04240"
     CDS             complement(13436..13843)
                     /locus_tag="PKK95_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HOC17452.1"
                     /translation="MQVTDLPALNATLNATAAGWLVLGYVLIRRGKRRAHRLAMLAAF
                     ATSSLFLLSYLVYHAQVGSRLFAGQGWIRPVYFAILISHVVLAASVLPLAIVTLTRGL
                     RARYDAHRRIARRTLPIWLYVSVTGVVVYLMLY"
     gene            complement(13847..14746)
                     /gene="cyoE"
                     /locus_tag="PKK95_04245"
     CDS             complement(13847..14746)
                     /gene="cyoE"
                     /locus_tag="PKK95_04245"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOC17453.1"
                     /translation="MSESPRPAVSSLPWPGTTAKASDFVALAKPRLNLLVVASTGAGF
                     FLAAERIDPAVLFHTIVGTALVAGGAAAFNEIIERDSDALMRRTRTRPLPTRRLEPAP
                     ATWFAFSLSILGLMQLAAGATTLAAGVALATLGSYVLVYTPMKRRSPMATLVGGIPGA
                     LPPVIGWTAASGELTAGAWVLFAIVFLWQMPHFLAIAWLCRDDYARAGLKMLPVVEPD
                     GRSTAQQTVLYAAVLVPVSLLPAVVGLAATLYFAGALVLGLAFFALAVRFAARRDRST
                     ALRLFLGSIVYLPLLWGILLLDH"
     gene            complement(14755..15780)
                     /locus_tag="PKK95_04250"
     CDS             complement(14755..15780)
                     /locus_tag="PKK95_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOC17454.1"
                     /translation="MIPRDCGTLRKASQSHSTRGARHMTWLHRSAVVLSACTLALLLA
                     GALVSSTGAGLALPGWPSLSPGAEAASGAWLQQGHRLLAALVALLTTLVAALAWRADP
                     RPWMKGLAVAAAALVVAQSVYGGLAVVSLLPAYFSVLHAALAQLFFGLTVAMALFTSP
                     GWLARERGAVPAESDPALARWALASTAAIYVQVLVGATMRHSYVADGRPGGLAIPDYP
                     LAFGQVVPVAELGSAIVAVAFLHRLIALVTAALVAVTVARVYRRHVGQAGLVRPATLL
                     ALLVGTQILLGGLAVLSGMTPLVTTMHAASVGIALAASLVLLLRAGAPTPLSAEARPR
                     RATDATA"
BASE COUNT         2476 a         5545 c         5654 g         2181 t
ORIGIN      
        1 ggtgctggtg caggaggtgc cgagggtgct ggtgcaggag gtgccgaggg tgctggcgct
       61 ggtggtgccg taggtgccgg tgcacgaggt gcaggaggtg ccgcagtgcg agtgccaggc
      121 cggggtgcag gcgcggcact ctcagcacca gcacttccgg caccatcagc actgcacctt
      181 cagcaccttc tgcacctcct gcacctcctg cacgacgaga tcgcaactac cgtcggaagc
      241 gattcttctg gataccgaac cttttgatct tcgactcgag ggtggtgggg gggatgccga
      301 ggcgggcggc ggcgccgccg ggccccgcga cgcggccgcc gcaggcctcg agcgccccct
      361 cgatcagccg cacctcctcg tcgtggagtg tgccggccgg ccgggcggcg cgggaggcga
      421 ggctgccgac gcccgcggtc aggagggtct cgtccaggtg caggatctcg ctgtccgaca
      481 ggatcacggc gcgctcgatg acgttctgca gctcgcggat gttgccgggc cacgcgtacg
      541 aacgcagcag tgccatcgtg gcgcggtcca cgccgcggaa ccggcggccc aaacgcgagc
      601 cgtgccgcgc gaggaagtac tcgacgagga tcgggatgtc ctcgcgccga tcgcgtagcg
      661 gcggaacgaa gagggggaag acgttcaggc ggaagaagag gtcgctgcgg aaacgccctt
      721 cggcgacggc ggccggcagg tcgcggttgg tggcggcgac gacgcgcgcc cgcgtgcgga
      781 tcgtctggtt gccgccgacc cgctcgaact cgccctcctg gaggacccgc agcaacgcca
      841 cctgcacgtc gcccggcagc tcgccaatct cgtcgaggaa gatcgtcccc tcgctggcca
      901 gctcgaaccg cccgatccgc cgctgcagcg ccccggtaaa ggcgcccttc tcgtggccga
      961 acagctccga ggcgaccagc gtgtcgggca tcgacgcgag gttcaccttg atgagcgcgc
     1021 ggccggcgcg cggcgagtgc cggtggatgg cgcgggccac caactccttg cccgtgccgg
     1081 tctcgcccgt gacgagcacc gtcgtgtcgg tgggggccac ccgctcgatt tcccgcacca
     1141 gctggcgcag cggcggcgac gagccgacga tctcctcgag catcgactgc tcgtccagct
     1201 cctgccgcag caccaggttc tcctcgtgca agcggttctt caggcgcccg atttcctcgt
     1261 aggccagggc gttcgacacc gcgccggcga tcggccgtgc aagctcctcg agcagcgccg
     1321 cctcgtcggg gctgaagggg ctcgacgaga ggcgcgcgaa ggtgagcgag ccgaccaggc
     1381 gcccccggaa tacgaggggc acgcggacat aggcgcggac gccgtacgac agcagccgct
     1441 cgtcctcggt gtaccgccgc gccgccgcca ggtcctgctg cacctcggcc cgcccggtgc
     1501 gcccgaccag ctcggtgccc gacccggcca gcgcgatcgg gcggccgtag cgagcctcgt
     1561 actcctctcg cgacaggtcg agcgcccggg cgatgctgcc gtgcacggtc tcgccctgtc
     1621 gggccgccac cccccggatg tggatggcgt gcggtctcag ctcggccccg tccaccgtga
     1681 cgatggcgat ggcgtcaagc gccacgagcg gttcgaggac ccgcccgact tcctcgagca
     1741 cgccggtcat gtcgagactc gagttggccg cctcggccac ctgcagcagg agccggtacc
     1801 tcgcctcgcc gctgagcatc cgcggaattg tacggcagaa tagcctctgg cgccgggcag
     1861 gtcgccttca cccgccagcc ttcccagggg agcttcggcg aggcggggag gcggtgagtg
     1921 ccggggccgc cggcagaaag ccgctggcag gccggggacg gaattcgcca cggcttggca
     1981 gcccagactt cgtgaagagg aggtggtgca tggagccgag cgtgatcgat ttcatcaaca
     2041 ccagccgcga ccggtacctc gaggagttga agcagtacct ggcgatcccc agcgtcagcg
     2101 cgctgcccga gcacgccggc gacttgcggc ggtgtgccga gtggacggcc gagcagatgc
     2161 gcgccatcgg cctcgagaac gtgcgcgtcg ccgacacccc ggggcacccg atcgtctacg
     2221 gcgactggct gcacgccgaa ggggcgccga cggccttggt ctacggccac tacgacgtcc
     2281 agccggtgga tccggtggag ctgtggacct cgcccccctt cgaggccacg gtccgcgacg
     2341 acgagctgtt cgcgcgcggc gcgaccgacg acaagggcca gctgttcgcc cacctgaagg
     2401 ccatcgaggc gcacctgaag caggccggcc gcctgccgat caacctccgc atcatcctcg
     2461 agggcgagga ggagatcggc agcccgaacc tgaaccagtt catcctcgac cacaagcacg
     2521 agctggcctg cgacgtcgtg ctcatctccg actcgccgat gttcgagaag ggggtgccct
     2581 ccatctgcta cggcctgcgc gggctggcct actgccagat cgacctgcgc ggcacgagca
     2641 ccgaccttca ctcgggctcc ttcggcggcg ccgtcgccaa cccggccttc gtactcgccc
     2701 aggtgctctc acagatgaag gaccgcggcg gccgcgtgaa gatccccggc ttctacgacg
     2761 acgtccgcga gctgaccgag cgcgagcgcg aggagttcaa gcggctgccg ttcaacgaga
     2821 aggagtaccg gaagggaatc ggcgcgccga agctccacgg cgagacgggg tactcgacgc
     2881 tcgagcggac ctgggcgcgg ccgaccttcg aggtgaacgg gctgctctcc gggttcaccg
     2941 gcgaggggag caagaccgtc attcccgcca ccgcgatggc caaggtgagc atgcggctcg
     3001 tgcccaacca ggatcccgac aagatcgcgg cgctgttcga ggactacgtt cggaaggtga
     3061 cgccgaagac ggtcgagctg aaggtgacgc gcatgcacgg cgggaagccg tggatgaccg
     3121 aactcgacaa ccccttcgtc caggcggccg cgcgggccat cgaggtgggg ttcaaccgga
     3181 cgccggtcta tacgcgggag ggaggctcga tcccgattgt cgccaccttc caggccgagc
     3241 tgaacgcgcc gacggtgctc ttcggcctcg gcacgccgga cgagaacgcc cacgccccca
     3301 acgagaagct ctacctgccg aacttctacg gcggcatcct gtcggcggcg tacctctacg
     3361 aggagatcgc gagagcgcgt cagtaggacc ccgcctcgct tgcgacgaaa ggccggaaaa
     3421 aagcgaaggg gcgcggcatc gtgccgcgcc ccttcacccc tcctgggccc ttggcctcca
     3481 acccttgcgc gtcacaccga tctgccgccg cgtctcatag ccagaggccc gaggcgtcgc
     3541 cggccgaatc gagtgagccc aagggagaac cgcgcgccgc cgccggagcg ccgccgccag
     3601 acgaatcggg tgaccgaagg ggagaaccgc gcgcgccgag agggtgccga tcgccccgtc
     3661 gtgggccgtg gatgggaaaa gacgcctgcc cagttgggct agcaggacca gtgccaattc
     3721 aggatgagct gaaatgcgcc aacattcggc cgacagcggc cgattcagtc caagatcttg
     3781 aaccccggcg ccgggccgat ccggagaatt ggggcagtat tttcggagga aatgacccag
     3841 gaagcgggcg ctacttcgtc ttctcacccg cgcggatggc cagcggcagc ctgttctcct
     3901 tgggggggaa tgggcagtcg taggaggcgt tgtagtagca gtacgggttg taggccctgt
     3961 tgaagtccac gacgtagatg ccggtgcgct gcggaacgag atccagatag cggccggccg
     4021 cgtaggtttc cttcccgctg gtatggtcgg cgaacggcac gaagaggcgg tcggccggct
     4081 gtccggcctc gacaaaggcg gtgagcgcca gccgctggcc gtcgagggtg aagcgcagcg
     4141 tgccgaggcg ttccatgagg cgcggcttgt cggccgaggt ttgaatgacc accctggtcc
     4201 gctgggcggg cggttcgggg tggaacgagg ccggcaccgc gtagcgcggg tcgggctggt
     4261 agtaggcgag cgggaggaag cggccgcggt cttccggctt gacgggggag ttggcggcct
     4321 ccgccagctc ggcgtccttt gccgcccgct cgcgcgcgag atccgccagg tacttcgcct
     4381 cgtctggggc gccgccggaa caggccgcca cgaacacagc cgcggcgagc aagccgactc
     4441 cagcgcctgg cccccagcca gtgcctcccc tcattccatg aacctctcga tcgcctcgga
     4501 catctcggtc agccgcccgt cgaacaggtg gttcgccgcg tcgatgacgg cgatgtcctt
     4561 gggctcggcg gcatgggcgt agaactgcca cagcgccggc gtggggcaga tctcgtcgcg
     4621 ttcaccctgg atgaagaact tcggcttggg cgagcgtgcc agcgggccga agtcgtagga
     4681 gtcgacgggc ggcgcaactg ccagcagccg cgtaacccgg gcgtcgtcgg ccccggccgt
     4741 cgtcgcgacc cacgcgccga atgagatgcc cgcgctccag atttgcacgc ccgggtggcg
     4801 cgcggcggcg aagtcgaggg ccgcccggta gtcgtcaatc tcgcccgggc cgttgctgaa
     4861 ggtcccgtcg ctgcgcccgg caccgcggaa gttgaagcgc accacggcgc agccgaggcg
     4921 gcagaacgcc ttggccgcct ggaacaccgc cttcgtgttc atcgttccgc cgtagacggg
     4981 gtggggatga gcgattacca cgacggcgcg cggcttgggc ggcgcggcgg ccgcctgctc
     5041 gggcgtgggc atggcagtcg tacagagcga ggcgggatcg tccacgagcg cttcgagacg
     5101 gccggcaggg ccggggatgt gatgggtcat tggcgtgtca cgcaagcccg aaactaggag
     5161 tgtgtctgag caaccagtgc acccgctcct gcgaagtcgg cgatcagccc ggcgaactcg
     5221 cgcggcctgg tcacgaggcc gaggtggccg gtgcccgcga gcgtccgcgc cgaggcgccc
     5281 gggatagtgg agacgtactc gagcgtctcg tccacgggta cgacgcgatc gaggtgcggc
     5341 tggccggtga tgacgagcgt gggcgaacgc acgcggcccg cgtcgacgcg aaagtcgagg
     5401 ccctcgaggc atcgaacccg gtttgccatg cgcgacggcc atgcgggtgc cagggcgacg
     5461 ttcacgagat ggcgcgcacc gcaggtgcag gcctgcagcc ggcttggggc cgccgcccgg
     5521 atctcgggcc agagccgcaa cggcgcgccc gcgacgaagg cgggcgccga gagccagggt
     5581 gacgaaacgt accgccggac ccgggccgcc ggccgccacc tgggcccggg ggccgacacc
     5641 agcaccagcg cgcgcgtgcg ctcgggacgg acggcggcgt agcgcaccgc gaccagcccg
     5701 ccgtacgaca cgccgcacac caccgcctgg ctccagccgt tctgccgcag gacgtcgtcc
     5761 acctgctcaa ggtgccgatc gaacggcgtg tcggggcgca gcggggcgtt gctgtccggc
     5821 tcgccgacca gcgagtaggt ggccacggtg aacgaggcgg cgagcgcgtg gacggcgggc
     5881 cgcatccact cccaccgccc ctggatgccc gggatcagca cgagcggcgg accggagccg
     5941 cagaggaaca tggatccatc atggcaggcc cgggcctcgc ggctcaaggc tgaggccgcg
     6001 tcaccgagcc agttccgatg ccgagggata gccctcgagg cccttcgcgg cgcggacggc
     6061 gcggaggatt gcctcggcga cggcctcggc agcgagcgag cccaccgtca gcaggtcggc
     6121 gggcttctcg tgcgtgccgg tggcgagtgc gaacagcgtg tcgccgtcga acggcgtgtg
     6181 ggcgggaacg acggcccgcg cgatcccgtc ttgcgccatc tgcgccacct tggtcgtctg
     6241 agtcttggtc agcctggcgt tggtggcgac gaccccgatt gtcgtgttct cgcccggctt
     6301 ggcgcgtggg acgaggctgc cgccgcgcag cagcgtccgc acatccgcca ggcgcctgcc
     6361 gtcctcggtg cggacgcccg ccaccacctg ccccgtcgcg ggatccacca cgtcgcccgc
     6421 ggcgttgacg gcaaccagcg ccgcgacgac gaggccgtcc ggcaggacca gggcggccgt
     6481 tccgaccccg cccttcatcg cacggtgctg gccgcccatc ttgcccacgg tcgccccggc
     6541 gcccgcgccg acgttgcctt ccgcgacagg gccggaggtt gccgcctgcg cagcccggta
     6601 gccgcagtcc gcggtcggcc ggatcttcgg gctgccaatc ccgaggtcga agaggatcgc
     6661 ggccggcacg atcgggacct tggccaccgc ggtctggtag ccgatgcccc gctcgtcgag
     6721 gaaacgcatc acaccgctcg cggcgtcgag gccgaaggcg ctgccccccg agagaaccac
     6781 cgcgtggacc tcctccacga ggttcacagg ctcgagaagg tcggtctcgc gcgtgccagg
     6841 cgccgagccg cgtacgtcca cgccggcaac cgcgccggcc tccgccaaaa cgaccgtgca
     6901 cccggtcggg cgctcgcgca gcgtgtgatg gccgaccttc aggccggcga cggcggtgag
     6961 gccttccggc ccctggggcg atggccgggg cgcgagtgtc tgggtcatgg cgagcatcag
     7021 tgggagagcc gcgacggccg aagccgcagc caggctggtg gtcattggcc tcatgatacc
     7081 ccgcgggctg caccaaccag cccgggcgtg atacggtacc ggcgccatga tcatccgacg
     7141 tttcacgccg gcggccgctg cggccctgct gctcgtttcc gctcaggtct actctcaatc
     7201 ccaatcccaa tcgggggccc aggccgtggg ggcggggcag gttgcccccg cgagggcagc
     7261 ggtgtcgggg ctcgaccctg cccggatggc gaggattgcc gagctggtca acgaggcgat
     7321 ggaggccaag gagctgcccg gggcggtcgt catggccggc cggggcgacg agatcctgta
     7381 ccagcaggcc ttcgggcgcc gggcggtcag gccctcctcg gagccgatga cgctcgacac
     7441 cgtcttcgac ctcgcctcgc tcaccaaggt ggtcgcgacc gcgccggcgg tgatgatgct
     7501 ggtcgaggac gggaaggtgc gcctgaacga gcgcgtctcg aagttcatcg aggggttcga
     7561 gcgctacggc aagggcgaca tcaccgtgcg gcacctgctc acgcacgtct cggggctgcg
     7621 gcccgacctc gacatggcgc tcgagttcga tagctacgac gaagcgatcg agcgggcgaa
     7681 ggaagaggtg ccgatcgcgc ggccaggaga gcggttcgtt tacagcgaca tcaacttctt
     7741 cctgctcgga gagatcgtcc gcaaggtgag cggccagggg ctcgaccagt tcgtgcgcga
     7801 gcggctgtac cagcccctcg ggatgctcga gaccaccttc aagcccggcg cctcgctggt
     7861 gtcgcggatt gcgccgaccg agccgtgcag cccgctcggg tggccgtgcg acacccccga
     7921 cgcgaggatg ctgcggggcg tcgtgcacga cccgacggcg cggcggatgc agggcgtggc
     7981 gggccacgcc ggcctcttca gcacggccgc cgacctgtcg atcttctgcc gcatgctgct
     8041 gaaccacggc cgctacgggc aggtgcgggt gctttcgccg ctcgcggtgg agaagatgac
     8101 cgggcccgtg ccggtgccgg gcggcgtgcg cggactgggc tgggacatcg acacgggcta
     8161 ctcgtcgaac cgcggggagc tgctgccgat cggctcgtac ggccacaccg gcttcaccgg
     8221 cacctcgatc tggatcgacc cggtcaccaa gacctggctc gtgttcctgt cgaaccgcgt
     8281 ccatccagat ggcaagggcg acgtcacccc gctgcgcgcc cgcatcgcca cgatcgtcgg
     8341 gtcggccatc ctcgaggttc cggccggcat cgcgcagccg ctgaggatga ccggcatcga
     8401 ccccggtccc accggcaccc gcatcccccg cgtcgggccg cccgtgctga acggcgtgga
     8461 cgtgctgaag gccgagaact tccggaggct ggccggccgc cgggtgggtc tcgtcaccaa
     8521 ccacactggc atcacggccg acggcgagag cacgatcgac cttctcgcgt cggcgaagac
     8581 cctctcactg gtcgcccttt tcagccccga gcacgggatc cgcggcacgc tcgaccagtc
     8641 cgtcatcaag tcggacaggg atcagaagac cgggctgccg atctactcgc tctatggcga
     8701 gacgaacagg ccgaccggcg agatgctgga agggatcgac acgctggtct acgacatcca
     8761 ggacgtgggc gtgcggttct acacctacga gacgacgctc gcctacgtgc tggaggaggc
     8821 ggcgaagcga aagatcaagg tggtcgtact cgaccgggtg aacccgatca acgggtggat
     8881 ggtggaggga ccggcgctcg agaaggagac cgcgagcttt gtcggctact tccccggctc
     8941 gccgatccgg cacgggatga cggtggggga actggcgctg ctgttcaacg cggagaagga
     9001 gattcgggcc gacctgagcg tcgtccccat cgccggctgg cggcgcgacc tctggttcga
     9061 cgacaccaac atgccctggg tcaacccgtc gcccaacatg cggaacctga tccaggcgac
     9121 gctctacccc ggcatcggcg ccatcgagta ctcgaacctg tcggtggggc gcggcaccga
     9181 cacgcccttc gagcaggtcg gtgcgccgtg gatcgacggc cgccgccttg ccgccaggct
     9241 gaacgcgcgg cagatcccgg gcgtcacctt ctatccgacc tggttcacgc cgtcttccag
     9301 caagtacaag ggtgagcggt gcggcggcgt ctacatcatg atcaccgatc gcgaagcgct
     9361 gcggccggtc cgcgtgggcg tggagatggc cgcggccatc cacggcctct acggcgacaa
     9421 gctccagctc gacccgaacg tgaagctctt cggcaccggc aaggtgctga cggcgatcaa
     9481 gaagggcgag gaccccgcgc gcatcgccgc ttcctgggcg gccgacgaag ggcgctggcg
     9541 gctgctgagg aacaagtatc tggtgtacag gtaggcgcag ggctcaaggc ttcgggcctc
     9601 gaggcgtttc agggcctggg gccttgagtc tcgagccccg agcccgtctc ctgccgcagg
     9661 ctcaccgtcc ccgtgaaggt ttccagctcg atccgggcgg cgccgttccc gagcgtcaac
     9721 gtgaagcgct tgcgccgcgc cggcaggtcg gggagcttca cggggagcac cgagtggacg
     9781 tgcccctcga acgaccggac ggccagcgtc gcgctcgcgg cgtcgggaag cgccaaatcg
     9841 atgtcgcccg agtgagtggc gagctggtag cggccgccgg ccgccaccgc tccatcccac
     9901 gcgatgtcgc cgctcacagt gctcgcggcg accgacgccg acttcaccga ccgcagcttc
     9961 accgttccgc tcaccgactc ggccaccagc tcgccctcga acccgttggc gcgcaccgca
    10021 ctgttcaccg attcgaggct ggcgcggccg tgccctccct cgagggtcac ttcgccctcg
    10081 gtcgacgcca gcacgatctc gcccgctccg cccgcgaccc tcacgtcgcc cctcaccgtg
    10141 cgcgctgtga ccgcggccgc ggtcccctcc accgagacgt cgaggtacgt gccggcgact
    10201 gccacgggca tccaggccgg cacatcgatc gtgaagtcga tcgcgtgcgg ctggcccctg
    10261 cgcgcgcgcg cacgcaccgt gatggtctgt ccctgtgcag cggcgtcgat ctcgtcggtc
    10321 gagaagtgcg tcgcgcgaat cctgacctcg tcgcgggccc aggcgcgcac cgcgatctcg
    10381 ccgccgaaca ggcgcaggtc gagggaggtt ccccgggcag cgggaaggat cttctcgaac
    10441 tcggccgctc ccagcgtcgc gccgagcacg agcgaggcgg caagcgcggc agcggcgcgc
    10501 acggcgggcc tcaattcccg gcctccgcgg cgaggtcgac ggcctgctgc agcacgtcga
    10561 tcttccgctg cctcgcggcc tcgaggcggc tggcgagccg cgagtcgccc ggctgctcgg
    10621 ccagggcgtc ggtgtaccgc gcaatcgcca tgtcgagcac ctcgaggttc tgctcgatca
    10681 cttccaccac ccgggggtcg cgcgcgaggc gcgcgcgggc ggcctgctgc agctgggcca
    10741 gcgtgtcgta gtactcgcgg cttgcctcga gtggccggag gtccccacgc ggttccggcg
    10801 gctgcggctc ggcaaccgag gcctgctcgg gcgcgacgga ccttccggcg cgaggaagca
    10861 cgagaccggc cgcgaccgcc aggaccagtg ccgcagcggc gaaggccggc cacagccggg
    10921 gccacgctgc cggccacgcc ggaacgaggg cgccgagccg cgcccgcggc gcgcggcgcg
    10981 gaccgagccg ctgctcgatt cccggccaga gatcggcggc cggctgctcg tcctgcctca
    11041 acgccggcgc ccgctgcacg acggcggcaa gctcctcgag cgcggcgcgg cactgcgcgc
    11101 agccgggcaa gtggtcctcg agagcgcgcc gctcgccggg cgacagctcc ccgtcgaggt
    11161 agtcagacag gaggccggtc caacggtccc cgctctcgcc cgccctcatc gcctctctcg
    11221 tcatgacgac tcgtccagcc cgcgaagcgc gccccggagc atcatcctgg cgcggtgcag
    11281 ctgccccttg gaagtgccgg ccgcgatccc cagcagcttc ccgatctcgc ggtgcttgta
    11341 gccctcgacg tcgtgcagca ggaacacctg ccgcgctccc tcgggcagcc tatccacggc
    11401 ggctctcaag tccatggcgg cgactccgtc gatgggcgcg acccctttca ggtcgagagc
    11461 catgccttcg tcgtgcgtct cgatcatcga ccgcctcgcc gccgaccgcc gccgctcgac
    11521 gatcgcgttg attgccagcc ggtagagcca ggtgccgaac tgcgagtcgc cccggaaggt
    11581 atccagcttc tcccaggccc ggacgaagac gtcctgcgtc agctcgtcgg ccgcgccgta
    11641 ccctgccatc cggcaggcga gcccgtgtat tcgccccacg ttcgccctgt acaggcgctc
    11701 gaaagcgccc tcgtcacctt ggcgcgccag ggccacatcg tcgggagcct cggtcggccg
    11761 tgtcgcccac gggtcggtca ggtcagggcc tccgggaaag aacgcggccg cgttcggtgt
    11821 ccctcgacac tttagatgtt tgccaggcgc ccgggggttg gaatccaacc atcgcgggca
    11881 cccggcccat ctaacggggc gagatgccaa tccgtccgac gagcttcggc ctccaatctt
    11941 cttctgttcg ggttgaggaa gccaggattc gtccggaacg attacgaaac cagaaaggac
    12001 gagtgtgcca tgaccagaag tcgagcggtg tttgcgtgtc tggtgctggt ggccctcgcc
    12061 ggcgtactgg cccagggcca ggcgaggtcg gtgcagggcg agtggaccgc gcgggttgaa
    12121 gacgacgggc gggtgcacct gcagctgcac accgacgacc tcgagccgag gcaggccggc
    12181 cgccagcctt cgcgcccccg cggcgactgg agcttcggcc ggtcgttcca gctttccgag
    12241 ctgaagggcc tgccggcgca gaagggcgcg ctcacggtgg agaacgccag gttcgagctg
    12301 ccgcgcgagg ccggcacgat cgccttccag gggagcttcc gggatggccg cggcgcgggg
    12361 ctgttctcgt tcacgccccg aagcgagtac gtcggcgaga tgaaggcgct cggctacgcg
    12421 gtggaggaca cttccgcctc cgggctgttc aggctggcgg ccttcgacgt cggaaggcgg
    12481 ttccacgacg agctcgcctc gctcggctac accaagctgc ccctcgagat gctggtgcgg
    12541 atgcgcactc acggcgccga cggcgagtac atcaaggagc tgcaggcggc gggctaccgc
    12601 gacgtgccgg ccgaccaggt cgtccgcgcg cgggaccacg gggtgacccg cgattacctc
    12661 gaggggttgg ctgccgccgg gtacaaaggg ctgccgctcg agcaggtggt gcgcgcccgc
    12721 gaccacggcg tcacgcacga ctacctcgag ggacttgcgg cttccggata caagggactg
    12781 ccgctcgagc aggtcgtccg cgcgcgcgat cacggggtga gcgcgaagta cctggaagag
    12841 ctgtcggcgg cgggcttcaa ggggctgccg ctcgacgagg tcatccgggc ccgcgaccac
    12901 ggcgtcaccc ccgagttctc gcgcgagctg cgccagctcg ggtactcgcc gtcgctcgac
    12961 gagctgcggc gccttcgcga ccacggcgtg acgcccggct acgtccggga gatgcgtgag
    13021 gccggctacg gcgacgcacc gctcgaggag ctgatccggc ttcgcgaccg cggcgtgacg
    13081 ggatcgttcg tgcgcgagat ggcccaggcg ggctacagca aggtcccgct ccagtcgctg
    13141 ctgcggatgc gcgatcacgg cgtgacgccc ggctacatcc gtgacctggc gggggtcggc
    13201 tacaaggaca tcgcgcccga tctcctggtg cgcatgcgcg accacggcgt cacgcccagc
    13261 ttcatccgcc gcgcgcagga gcagggccac aagaacgcgt cgcccgaaga gctgatccgc
    13321 ctccgctcgc gcggcattgt gcgggactga ctcagttcgc gaacttgact tggtgacttc
    13381 gcgacacgac ttcgcgactt cgcgacttct ccgactgctg actccgtgga ctgcctcagt
    13441 acagcatgag gtacaccacc acccctgtca ccgacacgta tagccagatc ggcagcgtgc
    13501 ggcgggcgat gcggcggtgg gcgtcgtagc gggcgcgcag tccgcgggtg agcgtgacga
    13561 tggcgagcgg caggacggag gcggcgagca cgacgtgcga gatcaggatg gcgaagtaga
    13621 cggggcgaat ccacccctgg cccgcgaaga gccgcgagcc cacctgggcg tggtagacga
    13681 ggtacgacag gaggaagagc gacgaggtgg cgaaggcggc gagcatcgcc aggcggtggg
    13741 cgcgccgctt cccccgccgg atcagcacgt agccaagcac cagccacccg gcggccgtag
    13801 cgttcagcgt cgcattgagg gcgggaaggt cggtaacctg catggctcaa tgatcaagca
    13861 gcaggatccc ccaaagcagc ggcaggtaca cgatcgaccc gaggaagagg cgcagcgccg
    13921 tcgaacgatc gcgccgggcg gcgaagcgga cggcgagggc gaagaacgcc agcccgagca
    13981 cgagcgcccc ggcgaagtag agcgttgcgg ccaggcccac cactgccggc agcaggctca
    14041 cgggaaccag caccgccgcg tagagcaccg tctgctgcgc ggtgctgcgg ccgtcgggct
    14101 cgactaccgg cagcatcttc agccccgccc gggcgtagtc gtcgcggcac agccaggcga
    14161 tcgccaggaa gtgcggcatc tgccagagaa agacgatcgc gaatagtacc caggcgcccg
    14221 ccgtcagctc cccgctcgcc gcggtccacc cgatcacggg cggaagggcg ccgggtatcc
    14281 cccccacgag ggtggccatg gggctgcgcc gcttcatcgg ggtgtagacg aggacgtagg
    14341 agccgagcgt tgcgagggca acaccggcgg ccagggtggt tgcgccggct gcgagttgca
    14401 tcaggccgag gatggagagc gagaacgcga accaggtcgc gggcgccggc tccagccgcc
    14461 gcgtcggcag cggacgcgtc cgcgtgcggc gcatcagggc gtcgctgtcg cgctcgatga
    14521 tctcgttgaa ggccgcggcc ccgcccgcca ccagggcggt ccccacgatg gtgtggaaga
    14581 gcacggcggg atcgatccgc tctgccgcga ggaagaagcc cgcgccggtc gatgcaacca
    14641 cgaggaggtt cagccggggc ttggccaggg cgacgaagtc ggaagccttc gcggtggtgc
    14701 cgggccaggg cagcgaggaa accgcgggcc gggggctctc ggacattggg gtgctcatgc
    14761 ggtggcgtcc gtggcccgcc gcggtctggc ctcggcggac aggggcgtgg gcgcaccggc
    14821 cctgagcagc agcaccaggg atgcggcgag cgcgatgccg acgctggcgg cgtgcatggt
    14881 ggtgacgagc ggcgtcatgc ccgacagcac cgcgaggccg cccaggagga tctgcgttcc
    14941 caccagcagc gccagcagcg tggccggccg caccaggccg gcctggccca cgtggcgccg
    15001 gtagacgcgc gcgacggtga ccgccaccag tgcggccgtc accagggcga tcagccggtg
    15061 caggaacgcc acggccacga ttgccgatcc cagctcggcc accggcacga cctggccgaa
    15121 cgccagcggg taatcgggga ttgcgagccc ccccgggcgc ccgtcggcca cgtacgagtg
    15181 cctcatagtg gcgccgacca gcacctgcac gtagattgcg gcggtcgagg caagggccca
    15241 gcgcgccagc gcagggtccg attccgccgg caccgcgccc cgttcgcgcg ccagccatcc
    15301 aggcgaagtg aagagggcca tcgccaccgt caggccgaag aacagctgcg cgagcgcggc
    15361 gtgcaggacc gagaagtagg cgggcagcag cgagacgacc gcgaggccgc cgtagaccga
    15421 ctgcgcgacc accagggcgg ccgctgccac cgcgagcccc ttcatccacg ggcggggatc
    15481 cgcccgccat gccaacgccg ccaccagcgt ggtcaacagc gccaccaagg cggccagcag
    15541 gcggtgaccc tgctgcagcc aggcgcccga cgccgcctcg gcgccggggg acaacgacgg
    15601 ccagcccgga agggccaggc cggccccggt gcttgaaacc agagcgccgg cgagcagcag
    15661 tgccagcgtg caggcgctga gcacgacggc ggatcgatga agccaggtca tgtgcctcgc
    15721 cccccgcgtc gaatgagact gtgaagcctt tctcaaggta ccacagtcgc gggggatcac
    15781 gaaccgtccg ggcccctagc ccctagcccc tagcccctag ctcctagccc ctagccccta
    15841 gctcctagcc cctagc
//
LOCUS       DAOPQX010000047        15441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4557_length_15441_cov_4.735084, whole genome shotgun sequence.
ACCESSION   DAOPQX010000047 DAOPQX010000000
VERSION     DAOPQX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15441
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4557_length_15441_cov_4.735084"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PKK95_04255"
     CDS             complement(<1..676)
                     /locus_tag="PKK95_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17455.1"
                     /translation="MKSRALRVMAVLLAVVALAAGGYLVFEAERQADEARAAQRTLED
                     DAVRLQAVIADLRAALPGYVASGQDPAYWARRVAALIDDLDARLKRLERPGAGTATAQ
                     EVATAREAAATLRQYDARIRSLVEQDRRVEASRLVFSDVNSLVMAASVALSQVVTLQR
                     TGANAALARGRKQQVYALAGAAALALVALLLLVPLPARWAAAPSSPPAPDEAVAPAGL
                     FGGPLVL"
     gene            complement(700..1509)
                     /locus_tag="PKK95_04260"
     CDS             complement(700..1509)
                     /locus_tag="PKK95_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOC17456.1"
                     /translation="MESLTSLLQSVSLERLSLLSMLVAFAAGVLSFISPCVLPLIPGY
                     ISFISGASLEQMRGRPATVAAEAGAGVATAPAPGTSRRILLTSLAFVAGFSLVFVAFG
                     ATASAIGQLLGSHKAKIAYVAGALLIVLGLHMMGIFRIGFLDYEKRARASKRPTGLLG
                     AGLVGIAFAFGWTPCIGPILGGILTIAGSQESVAQGVLLLGAYSLGLGVPFLLTAVAI
                     DRFFAAFAKIRRHYRAIEMVSGALLIGIGLLMLFGRMTAITNALTPYLPTF"
     gene            complement(1534..3318)
                     /locus_tag="PKK95_04265"
     CDS             complement(1534..3318)
                     /locus_tag="PKK95_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOC17457.1"
                     /translation="MDTVTLTIDGRSITVAKGTTVLQAAIEHGIQIPYYCYHPALGID
                     GSCRVCIVKIEKMPKLQTACSTLCADGMVVDTCSPEVVEARAGVFEFLLINHPLDCPV
                     CDKGGECPLQDYSYAFGPDASRMDFPRRTFDGEGVRADVDFGPTLLINRNRCILCSRC
                     VRFMRDIDGDAQIGVEDRGYGSHIVTFQDKGVHSILSGNLMDVCPVGALLARDYRFKS
                     RPWDNPVAVDTICTLCAKGCSITAWLKAKPEWAKGPRLARVTPRHNPQVNDYWMCDIG
                     RFEYHFAESDDRLRKPLRRTEAGLLQPEGWKEVLVKLREAVGAADAPGERRTIAFLAS
                     AHASIEELVLLGQIARGLNGDGAERQIGVSWRVSEKVQPAATKFKVPRVDAPNVAGAA
                     DLGVGAPPLENGGPDLSALQADVEAGRVDVLYVLDPGPAGSIGDVSWAIEARAQGRIR
                     LLVVQGVLMTDLAAAADLVLPGATYLEKDACYTNEQGLVQAASRAVAPPGDAMEDWQI
                     LVNVGVTLGVGLPYTSAAHIRADVAAAMPGRPGYADLPRLAFARPAVARTWLQTSNPS
                     ERWKWDALFKDLPPVKFKGKAEAEDREP"
     gene            complement(3354..6062)
                     /locus_tag="PKK95_04270"
     CDS             complement(3354..6062)
                     /locus_tag="PKK95_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOC17458.1"
                     /translation="MLKRLSLVSAALVVTLWQPVPARRLQDQDGVASLLARLEEVLRA
                     GRPDGYLALLSNTADRQRARQFAEGNVAGPSSRVVVQERDREALVGTLPGDGYQLSVE
                     VLVENGNRARLSTWRLDVRRRGGDLDEWGIANQVPLTSIYGLYRLGLNPAKQYAARNL
                     VVQDEDLRLTLAEGTAFVAEVAGEGSATALVLLGRGEMTFAPPAPTERAQLRLFADAQ
                     SLQSPFEAAFVRLNPGSLPAFLDPAALTPVPVDPRELKRAEEVFRQDVGNSFGLDLGD
                     LSPDSWSLIPGLGDLLVEMRTRRFDTLTYARSLGEVEDVSLFDRRNRRNISVYSSRRN
                     AARYGRSYSEDDRADYAVLDYDIEASFNPARTWVEGRTVLSIEVKADALNSLSFRLAD
                     PLEVQSVTSLEFGRMLALRVRDQNGIVVNLPSTITRGYRMMIAIEYSGTLPPQPIDRE
                     ALAIEPQMVPIQQVDEFERIPVEESFLYSNRSYWYPQPSSPNYATARLRLSVPATYGM
                     AASGDLVTVVSPPAPRGTQAIHQHHYQVHQPGRYFACLISPLREVKRERIDLGDAVAP
                     FRALRRPGVYYDTVLMTVRTQPRLQRRGRDLAATTKDILKFYSSLVGEAPYSALTLAV
                     VERGLPGGHSPPYMAVLVQPSVSSRLVYRDDPASFSDFPEFFLAHELAHQWWGQGVGW
                     KNYHEQWISEGFAQYFTALYAQKARGNAVFGSVMRRMARFGVDQSAAGPVWLGYRVGH
                     IRGDSRAFRAVVYNKSAIVLHQLRGIVGDEVFFRGVRRFFDAWRFKKAGTEEFRAAME
                     AEAGRPLDRFFEQWIYGDAVPRVEVSWREETQDGGTRQAVVRVEQVGEEAFEFPLQLS
                     VVYAGRAPASTTVKVADRVTEARIPLTGPVRAIEANRDGTALVVVK"
     gene            complement(6066..7253)
                     /locus_tag="PKK95_04275"
     CDS             complement(6066..7253)
                     /locus_tag="PKK95_04275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOC17459.1"
                     /translation="MARKHHHPGRARRFIDTYTSGLTGEELRRVFTRDAPQAYRYFSR
                     GMDEKAIAALPVHKQLALRARLFFWAFTLKLSPARRAIYGAGLLLALLGFLQLFRGIG
                     FLSLPLPFSPGGGDLQVGVPAPDFADGALTLFIAFLLINLLVLLEVADRLSLKNDLEV
                     ARGIQSAMLPQATYSDPCLEAFGLTRPANTVGGDFYDVLPRPDGRLVVALGDVAGKGS
                     PAALLMALLLAIFRTLLDEGLEGADLARRLNAQVCRHAPPSRFITLFMAACDPCTGRL
                     VYVNAGHMPALVRRNSGEIERLTEGDVALGMFEHSTYSAHETWLQTGESVVLYSDGVT
                     EAENHQGRPFEESGLIKVVQAYSAAGPRDVAHAVLRLVEEHAQETSLADDVTLLVLKR
                     RKA"
     gene            7388..7780
                     /gene="acpS"
                     /locus_tag="PKK95_04280"
     CDS             7388..7780
                     /gene="acpS"
                     /locus_tag="PKK95_04280"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOC17460.1"
                     /translation="MIVGLGTDLVDVARFRRVADRYGDHFLESLFSPAEIGWCRRRRR
                     PWEHLAARFAAREAVLKALGTGLVGGMSWKDIELVPGGGPGTCAMELRRQVRDAADRL
                     GVRHVHVAMSVGRDVAAATVVLESSAGL"
     gene            7827..8084
                     /locus_tag="PKK95_04285"
     CDS             7827..8084
                     /locus_tag="PKK95_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOC17461.1"
                     /translation="MKDVILEYVKNEYLDEDEADEIGLDENTPLITSGIVDSFSMVSL
                     KRFLEKKYGIAIPDDQATPEAFNTVASIVELVKRHQVGKGA"
     gene            8086..9333
                     /locus_tag="PKK95_04290"
     CDS             8086..9333
                     /locus_tag="PKK95_04290"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR01822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOC17462.1"
                     /translation="MAYPAALREAYRGELQGIREKGIYKEERYIHSPQAAGIDVEFPR
                     GAGLKRVVNLCANNYLGLSSHPEVVAAAHRGLDERGYGMSSVRFICGTQDIHRELEKG
                     LTAFLGTEDTILFPSCMDANGGVFEAVLNDQDVMIADRLVHASIVDGMRLCKAMQDTY
                     KHADMKHLEQKLQEHQDKRARLIITDGVFSMDGDLAPLDQIVSLAERYQAMVFVDDSH
                     ASGFMGKTGRGTPEHFGVHGKIDIITTTLGKALGGASGGCVSGRQEIVDLCRQKARPY
                     LFSNAVPPPIVYAAQKVLEILSASTERRDKLEANTAFWRKGLKEAGFVIKDGESPIVP
                     IMLFNAKLSQDIARDLYAEGIYVIGFFFPVVAAGQARIRTQLSADHDIPMLADALEAF
                     KRVGAKYGILGLDKKGIIEKYGL"
     gene            9527..10726
                     /locus_tag="PKK95_04295"
     CDS             9527..10726
                     /locus_tag="PKK95_04295"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOC17463.1"
                     /translation="MSKIAMKTPLVEMDGDEMTRILWAWVKAKLIEPHVDLKTEYYDL
                     GLEHRDRTDDRVTHEAAEATRRLGVAVKCATITPNEARMSEYKLKKQWKSPNATIRAA
                     LDGTVFRKPILVPNIKPGVVNWKKPIIVGRHAYGDVYKNAEIAVPGPGKGELVFTGED
                     GTEKRVLIHEFGGPGILQAIHNTEASIRSFARSCFTYALDEKVDVWFATKDTISKTYD
                     ATFRRVFEEVFGQEFATRFKTAGIEYFYTLIDDVVARVVKGDGGMLWACKNYDGDVMS
                     DMVASAFGSLAMMTSVLVSPDGFFEYEAAHGTVQKHYYKHLKGEKTSSNSMALIFAWT
                     GALRKRGELDGLGDLAEFATRLEQASLATIAEGVMTGDLARLASPPPRQVVGSEGFLD
                     AIAGRLC"
     gene            10731..12113
                     /locus_tag="PKK95_04300"
     CDS             10731..12113
                     /locus_tag="PKK95_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOC17464.1"
                     /translation="MDPYQQYADDIVGRAAAAAEAWRRFDQAQADAVVEAMAAAAWEA
                     RENLARLAVDETQMGVFGDKVLKNAWAAAIVRDSLRGTRTAGVIAQDEASGITEFAEP
                     VGAVLGLVPVTNPTSTAIFKALVCAKTRNALIFSPHRGARKCVRETARILSEAATGAG
                     APEDAIQVVSRSQTEYLRQVMVHKRLALIVATGTRSIVEAARHSGTPTLGVGPGNVPV
                     YVHGSADLAEAARRILLSKTFDNGTVCASEQAVVVESPQAAALREAFERGGAHFCSAA
                     EVEALGPVCFEASHRRMRADVVGQPAAEIARRAGFRVPARTRLLVGAPGGIGPSHPLS
                     AEILAPVLAWYEVRSYAEALRTCRAITAWGGVGHTVGVHARDESVVADFAQMRASRVL
                     VNQPTTQGAIGGIVNGLQPSLTLSCGPSARNLTTDNIGARHLLNIHRVVLPRANPAWA
                     ELTTGPADRV"
     gene            complement(12118..12450)
                     /locus_tag="PKK95_04305"
     CDS             complement(12118..12450)
                     /locus_tag="PKK95_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17465.1"
                     /translation="MLSRWIFNRSGASGFGNVAVVAFLAVQALDGVLTYVGLATLGSH
                     MEGNPVVASLMLAFGTGPGLTGAKLVAGVLGIALHLTGVHRLVALLTAIYLGAAILPW
                     TALLLMAG"
     gene            12778..13764
                     /locus_tag="PKK95_04310"
     CDS             12778..13764
                     /locus_tag="PKK95_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC17466.1"
                     /translation="MNGKRVASVVLRMFPLARVLLGMVMVGLAAPVAAQAPAVASPSG
                     ATFRSGVDMVTVSAVVRDRKGRIVKNLRRSDFELLDSGAPKAIRDFRPDQAPISVAVL
                     VDGSGSMQVADKIAEARAAAQYLFAWLDPGRDEVGIFSFDTGLYELRPFATADEPAGL
                     QATLAGVMPFGMTSLHDAVAATARVVAERANVHRAVIVVTDGVDNGSRLTPAEVSGIA
                     SSIDVPVYIVAVVSPLDHAGASSAVRSERPVPVGDLADLARWTGGELYVSSSAAHTSA
                     ATREIVEELRHQYLIAFEPGTRPGWHPLEVRTRKDNLIVRARSGYMAGQAQD"
     gene            13896..14561
                     /locus_tag="PKK95_04315"
     CDS             13896..14561
                     /locus_tag="PKK95_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOC17467.1"
                     /translation="MRKFVLAMSVASIAAMGSTACATKGFVRDRVGEVNDKVETVSRS
                     LEETQERTRKNEARIGEAEQKIQQVDQRAAAANTAATEARTAADTAANKAEAVEKSVK
                     RIVYEVTLSEDQGNFKFGQAALPDEAKARLDEVASQLKANPNGAFIEVEGYTDSVGSK
                     EYNQQLGLQRAESVKRYLYSQHQIPLHRINVISYGAENPIAPNNTRAGRAKNRRVVIK
                     VLV"
     gene            14731..15114
                     /locus_tag="PKK95_04320"
     CDS             14731..15114
                     /locus_tag="PKK95_04320"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOC17468.1"
                     /translation="MAIRTELTLRLQNSPGMLARVCQVIANERINIVAMNLASPLVVR
                     LVVDNPVHAAAVLREHQYQVEERDVIYTATSNDPGALLRITRLIADAGINVEYLYATA
                     LEGQSMASVVVGVPDAERASSAAGL"
     gene            15124..>15441
                     /locus_tag="PKK95_04325"
     CDS             15124..>15441
                     /locus_tag="PKK95_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17469.1"
                     /translation="MNDSPASGPATSRPRRRPGRLLVPLKTLRVRLALLVTLVVALVV
                     GASSYLQMRVFESEISAGLEDTAIKIAVAVADDVEVRPEPLNHGALAESLREFYEAVP
                     SLRA"
BASE COUNT         2495 a         5187 c         5588 g         2171 t
ORIGIN      
        1 cgagcacgag cggcccaccg aaaaggcccg ccggtgcgac ggcctcgtcg ggcgcgggcg
       61 gcgaggaggg agcggcggcc caccgggcgg gcagcggcac caggagcagc agcgcgacga
      121 gcgcgagcgc cgcggctccg gccaaggcgt agacctgttg tttccggccg cgggccagcg
      181 cggcgttggc gccggtccgc tgcagcgtca ccacctgtga gagggcgacc gaggcggcca
      241 tcaccagcga gttgacgtcg gagaagacca gccgcgaggc ctcgacgcgg cggtcctgtt
      301 cgacaagcga acggatccgg gcgtcgtact gccgcagggt ggcggcggcc tcgcgcgcgg
      361 tcgcgacctc ctgggcggtg gccgtgccgg cgccggggcg ctcgagccgc ttcaggcggg
      421 cgtcgagatc gtcgatgagc gcggcaaccc ggcgcgccca gtaggcgggg tcctgccccg
      481 acgccacgta gccgggcagg gcggcgcgca ggtcggcgat gacggcttgc agccgaacgg
      541 cgtcgtcctc gagcgtgcgc tgggcggcgc gcgcctcgtc ggcctgccgt tccgcttcga
      601 agacgaggta acccccggca gcgagggcga cgacggccag caggacggcc atcaccctga
      661 gggcccggct cttcattgat cgcagactgt atcagtgcgt cagaaggtcg gcaagtacgg
      721 cgtcagcgcg ttggtgatgg cggtcatgcg gccgaagagc atcagcaggc cgatgccgat
      781 cagcagcgcg ccggagacca tctcgatggc gcggtagtgg cggcggatct tcgcgaaggc
      841 ggcgaagaac cggtcgatgg cgacggcggt cagcaggaac gggacgccga ggccgagcga
      901 gtaggcgccg agcagcagca ccccctgggc gacgctctcc tggctgccgg cgatcgtcag
      961 gatgcccccg aggatcgggc cgatgcaggg cgtccagccg aaggcgaagg cgatgcccac
     1021 cagccccgcg ccgagcaaac cggttggccg cttcgaggcg cgcgcccgct tctcgtaatc
     1081 gaggaagccg atgcggaaga tccccatcat gtgcaggccg agcacgatca gcagcgcgcc
     1141 ggccacgtag gcgatcttcg ccttgtggga gccgagcagc tggccgatgg cgctcgcggt
     1201 ggccccgaag gcgacgaaga cgagcgagaa cccggcgacg aaggccagcg aggtgagcag
     1261 gatccgccgg ctggtgccgg gcgccggcgc cgtcgccacc cccgcgcccg cctcggccgc
     1321 caccgtcgcc ggccgcccgc gcatctgctc gagcgacgcc ccggagatga acgagatgta
     1381 gcccgggatg agcggcagca cgcagggcga gatgaacgac aggacgccgg cggcaaacgc
     1441 caccagcatc gagagcagcg agagccgctc gagggaaacg gactggagga gcgaggtgag
     1501 ggactccatg tcgtcagtaa tggcagatga agattacggc tcgcggtcct ccgcctccgc
     1561 cttccccttg aacttcaccg gcggcaggtc cttgaacagc gcatcccact tccagcgctc
     1621 ggaggggttc gatgtctgca gccacgtccg cgcgacggcg ggtctcgcga aggccaggcg
     1681 cggcaggtcg gcgtagccgg gccggccggg catcgcggcg gccacgtcgg cgcggatgtg
     1741 cgccgcgctg gtgtagggga ggccgacgcc gagcgtcacc cccacgttga ccaggatctg
     1801 ccagtcctcc atcgcgtcgc cgggcggcgc gacggcgcgc gaggccgcct ggacgagccc
     1861 ctgctcgttg gtgtagcacg cgtccttttc caggtaggtc gccccgggca ggacgaggtc
     1921 ggcagcggcg gcaaggtcgg tcatcaggac gccctggacg accagcagcc tgatgcgccc
     1981 ctgcgcgcgc gcctcaatcg cccacgacac gtcgccgatc gagccggcgg gcccggggtc
     2041 gaggacgtag agcacgtcca cccgccccgc ctccacgtcg gcctggaggg cggagagatc
     2101 ggggccgccg ttctcgagcg gcggggcgcc gacgccgagg tcggccgcgc cggcgacgtt
     2161 cggggcgtcc acgcgcggca ccttgaactt cgtcgcggcg ggctgaacct tctcgctgac
     2221 gcgccagctg acgccgatct gacgctcggc cccgtcgccg ttgaggccgc gcgcgatctg
     2281 gccgagcagc accagctcct cgatcgaggc gtgcgccgag gcgaggaagg cgatcgtccg
     2341 ccgctccccg ggcgcgtctg ccgccccgac ggcctcgcgc agcttgacca ggacctcctt
     2401 ccatccctcg ggctgcagca ggccagcttc ggttcgacgg agcggcttcc gcagccggtc
     2461 gtccgactcg gcgaagtggt actcgaaccg gccgatgtcg cacatccagt agtcgttcac
     2521 ctgcgggttg tggcgcgggg tgacgcgtgc cagccgcggc cccttcgccc actccggctt
     2581 ggccttcagc cacgcggtga tgctgcaccc cttcgcacac agcgtgcaga tggtgtccac
     2641 cgcgaccggg ttgtcccagg ggcgcgactt gaaccggtag tcgcgcgcca gcagcgcgcc
     2701 gaccgggcag acgtccatca ggttcccgct gaggatcgag tgcaccccct tgtcctggaa
     2761 cgtcacgatg tggctgccgt acccgcggtc ctcgacgccg atctgtgcgt cgccgtcgat
     2821 gtcgcgcatg aaccggacgc agcgcgagca gaggatgcag cggttgcggt tgatgagcag
     2881 cgtggggccg aagtcgacgt cggcccgaac cccctcgccg tcgaaggtgc ggcgcgggaa
     2941 gtccatgcgg ctcgcgtcgg ggccgaaggc gtaggagtag tcctggagcg ggcactcgcc
     3001 gcccttgtcg cagaccgggc agtcgagcgg gtggttgatg agcaggaact cgaacacgcc
     3061 ggcgcgcgcc tcgaccacct cgggcgagca cgtgtccacc accatgccgt cggcgcacag
     3121 cgtcgagcac gccgtctgca gcttcggcat cttctcgatc ttcacgatgc agacgcggca
     3181 cgagccgtcg atgccgaggg cgggatggta gcagtagtag gggatctgga tcccgtgctc
     3241 gatggcggcc tggaggacgg tcgtcccctt ggcgaccgtg atcgaacggc cgtcaatcgt
     3301 cagcgtgacg gtgtccatat gggggaaatg atagcagaca ggctcagggc tccctacttg
     3361 accaccacga gcgccgttcc gtcgcggttg gcctcgatgg cgcgcacggg ccccgtcagc
     3421 gggatgcggg cttccgtcac ccgatcggcc accttcaccg tcgtgctcgc gggcgcgcgg
     3481 ccggcgtaaa cgacgctcag ctggaggggg aactcgaagg cctcctcgcc aacctgttcc
     3541 acgcgcacga ccgcctgccg cgtcccgccg tcctgcgtct cctcgcgcca gctcacctcc
     3601 acgcgcggga ccgcgtcgcc gtagatccac tgctcgaaga agcggtcgag cggcctgccg
     3661 gcctcggcct ccatcgcggc ccggaactcc tcggtcccgg ccttcttgaa cctccaggcg
     3721 tcgaagaacc gccggacgcc gcggaagaac acctcgtcgc ccacgatccc gcgcagctgg
     3781 tgcagcacga tcgcgctctt gttgtagacc accgcgcgga acgcccggct gtcgccgcgg
     3841 atgtgcccca cccggtagcc gagccagacc ggccccgcgg cggactggtc gacgccgaag
     3901 cgcgccatcc ggcgcatcac gctgccaaag accgcgttgc cgcgcgcctt ctgggcgtac
     3961 agggcggtga agtactgtgc gaacccctcg ctgatccact gctcgtggta gttcttccag
     4021 cccaccccct gcccccacca ctggtgcgcc agctcgtggg cgaggaagaa ctcggggaag
     4081 tcggagaacg acgccgggtc gtcgcggtag accagcctgg acgacaccga gggctgcacg
     4141 agcaccgcca tgtacggcgg gctgtgcccc ccggggaggc cgcgctccac gaccgcgagc
     4201 gtcagcgcgg aatacggcgc ctccccgacc agcgagctgt agaacttgag gatgtccttg
     4261 gtcgttgcgg cgaggtcgcg gccgcgccgc tggagccgcg gctgcgtgcg cacggtcatc
     4321 agcaccgtgt cgtagtagac gccgggacgc cgcagcgccc ggaacggggc aacggcgtcg
     4381 ccgaggtcga ttcgctcgcg cttcacttcg cgcagcggcg agatgaggca cgcgaaatag
     4441 cgccccggct ggtgcacctg gtagtggtgc tggtggatcg cctgcgtccc cctcggcgcc
     4501 gggggagaga ccacggtcac caggtcgccg ctggcggcca tcccgtaggt ggccggcacc
     4561 gacagccgga ggcgggccgt ggcgtagttg gggctcgagg gctgcgggta ccagtagctg
     4621 cggttgctgt agagaaagct ctcctcgacc gggatccgct cgaactcgtc gacctgctgg
     4681 atcggcacca tctgcggctc gatcgccagc gcctcgcggt cgatcggctg cggcggcagc
     4741 gtgcccgagt actcgatcgc gatcatcatc cggtaaccgc gcgtgatcgt ggaaggcagg
     4801 ttgacgacga tgccgttctg gtcgcggacg cgcagggcca gcatccgccc gaactcgagc
     4861 gaggtgaccg actgcacctc gagcggatcg gccagccgga acgagagtga gttgagggcg
     4921 tcggccttca cctcgatcga gagcaccgtc ctcccctcga cccaggtgcg cgccgggttg
     4981 aagctcgcct cgatgtcgta gtcgagcacc gcgtagtcgg cgcgatcgtc ctcgctgtag
     5041 gagcgcccgt accgcgccgc gttccgcctc gagctgtaga ccgagatgtt gcgccggttc
     5101 cgccggtcga acagcgagac gtcctcgacc tcgccgagcg accgcgcgta ggtcagcgtg
     5161 tcgaatcgcc gcgtgcgcat ctccaccagg aggtcgccga gccccgggat cagcgaccag
     5221 gagtcggggc tgagatcgcc gaggtcgagg ccgaacgagt tgccgacgtc ctgccggaac
     5281 acctcctcgg cccgtttcag ctcgcgcggg tccaccggca cgggggtcag ggcggccggg
     5341 tcgaggaagg ctggcaggct gcccgggttc agccggacga acgcggcttc gaagggcgac
     5401 tgcagggact gggcgtctgc gaagaggcgc agctgggccc gctcggtcgg ggcgggcggc
     5461 gcgaaggtca tctcgccgcg gccgagcagc acgagcgcgg ttgccgaccc ctctccggca
     5521 acctcggcca cgaaggccgt cccctcggcc agcgtcagcc ggagatcctc gtcttgaacg
     5581 acgaggttgc gcgcggcgta ctgcttggcg gggttcagcc cgaggcggta cagcccgtag
     5641 atcgaggtca gcggcacctg gttggcgatg ccccactcgt cgaggtcgcc gccgcgccgg
     5701 cgcacgtcga gccgccaggt ggacaggcgg gcacggttgc cgttctccac cagcacctcg
     5761 acgctcagtt ggtacccgtc tcccggcagc gtgccgacca gcgcctcgcg gtcgcgctct
     5821 tgaacgacca cccgggagct ggggcccgcg acgttcccct ccgcgaactg ccgcgcccgc
     5881 tggcggtcgg ccgtgttgga cagcagagcg aggtacccgt ccggccgccc cgcccgcagc
     5941 acctcctcga ggcgcgccag cagcgacgcc accccgtcct ggtcctggag acggcgggcg
     6001 ggaacgggct gccagagcgt caccacgagc gccgcggaga ccagggagag cctcttgagc
     6061 aaacgctacg cctttctcct tttcaggacc aacagcgtca catcgtcggc cagcgaggtt
     6121 tcctgcgcgt gctcctcgac caggcgcagc accgcgtgcg ccacgtcgcg ggggcctgcg
     6181 gcggagtagg cctgtacgac cttgatcagt ccgctttcct cgaacggccg gccctggtgg
     6241 ttctctgcct cggtgacgcc gtcactgtat aagacgacgg attcgccggt ttggagccac
     6301 gtttcgtgcg cggagtacgt cgagtgctcg aacatcccga gggcgacgtc cccctcggtg
     6361 agccgctcga tctcccccga gttccggcgg acgagggcgg gcatgtgccc ggcgttcacg
     6421 taaaccaggc ggccggtgca ggggtcgcag gcggccatga agagggtgat gaaccgcgac
     6481 gggggcgcgt ggcggcagac ctgtgcgttg aggcggcggg ccaggtcggc gccctcgagc
     6541 ccctcgtcga gcagggtgcg gaagatcgcg agcagcagcg ccatcagcag ggcggccggg
     6601 ctgcccttgc cggccacgtc gcccagggcc acgaccaggc ggccgtccgg ccgcgggagc
     6661 acgtcgtaga agtcgccgcc gaccgtgttg gcgggccggg tgaggccgaa cgcctccagg
     6721 caggggtcgc tgtaggtggc ctggggcagc atcgccgact ggatgccccg cgccacctcg
     6781 aggtcgttct tgagcgagag gcggtcggcc acctcgagca ggacgagcag gttgatcagg
     6841 aggaaggcga tgaacagggt cagcgcgccg tccgcgaagt cgggggccgg gacgccgacc
     6901 tggaggtcgc cgccgcccgg cgagaacggg agggggagcg acaggaagcc aatcccccgg
     6961 aacagctgca ggaagccaag gagggcgagc agcagtccgg cgccgtagat ggcgcgccgg
     7021 gccggcgaca gcttcagtgt gaaggcccag aagaacaggc gggcccgcag cgcgagctgc
     7081 ttgtgaaccg gcagcgcggc aatcgccttc tcgtccatcc cccgcgagaa gtagcggtaa
     7141 gcctggggcg cgtcgcgcgt gaagacgcgg cgcagctcct cgccggtcag cccgctcgtg
     7201 taggtgtcga tgaagcggcg cgcgcgtccg ggatgatggt gcttcctggc catgcggctc
     7261 ctggcgtcag atcccaagtc tgtcactttc tcacatccga cccccggcgt cagccggtgg
     7321 atgaggggcg aatcgggatc tggcgctggt cagtggcaaa tgatactccg gtgtatgcta
     7381 gacgctcatg atcgtcggcc tgggcaccga cctggtggac gtcgcccggt tccgccgggt
     7441 cgccgatcgt tatggcgacc acttcctcga gagcctgttt tccccggccg agatcggatg
     7501 gtgccgccgc cggcggcgcc cgtgggagca cctggcggcc cgcttcgccg cgcgcgaggc
     7561 ggtgctgaag gcgctcggga ccggcctggt gggcgggatg tcgtggaagg acatcgagct
     7621 ggtgccgggc gggggaccgg gcacctgcgc gatggagctg cggcggcagg tgcgcgacgc
     7681 ggccgaccgc ctgggcgtgc ggcacgtgca cgtggcgatg agcgtggggc gcgacgtggc
     7741 cgcggccacc gtggtactcg aaagtagcgc agggctttag caggttgctc aggaaaagga
     7801 cgggaacgcc aacgggtgga gccatcatga aggacgtcat tctcgaatac gtgaagaacg
     7861 agtacctcga cgaggacgag gccgacgaga tcggcctcga cgagaacacg ccgctcatca
     7921 cgagcggcat cgtcgacagc ttcagcatgg tgagcctgaa gcggttcctc gagaagaagt
     7981 acggcatcgc cattcccgac gaccaggcga cgcccgaggc gttcaacacc gtcgccagca
     8041 tcgtcgagct ggtgaagcgc caccaggtcg ggaagggggc ctgagatggc gtacccggcc
     8101 gcgctgaggg aggcctaccg cggcgagctg cagggcatcc gcgagaaggg catctacaag
     8161 gaagagcgct acatccactc gccgcaggcc gccggcatcg acgtcgagtt cccgcggggc
     8221 gccgggctga agcgggtggt gaacctgtgc gccaacaact acctcgggct gtcgagccat
     8281 cccgaggtgg tcgcggccgc gcaccggggc ctcgacgagc gcgggtacgg catgtcgagc
     8341 gtccgcttca tctgcggcac gcaggacatc caccgggagc tcgagaaggg gctcaccgcc
     8401 ttcctcggca ccgaggacac gatcctgttc ccgagctgca tggacgccaa cggcggcgtc
     8461 ttcgaggcgg tgctgaacga ccaggacgtg atgatcgccg accgcctcgt gcacgccagc
     8521 atcgtggacg ggatgcggct gtgcaaggcg atgcaggaca cctacaagca cgccgacatg
     8581 aagcacctgg agcagaagct ccaggagcac caggacaagc gtgcgcggct gatcatcacc
     8641 gacggcgtgt tcagcatgga cggcgacctg gcgcccctcg accagatcgt cagcttggcc
     8701 gaacgctacc aggcgatggt gttcgtcgac gacagccacg ccagcggctt catgggcaag
     8761 accgggcggg ggacgccaga gcacttcggg gtgcacggga agatcgacat catcaccacc
     8821 actctgggca aggcgctggg cggcgcgagc ggcggctgcg tgtcgggccg gcaggagatc
     8881 gtggacctgt gccggcagaa ggcgcggccg tacctgttca gcaacgccgt tccgccgccc
     8941 atcgtctacg cggcgcagaa ggtgctcgag atcctgagcg ccagcaccga gcggcgcgac
     9001 aagctcgagg ccaacacggc gttctggcgg aaggggctga aggaagcggg gttcgtcatc
     9061 aaggacggcg agagcccgat cgtcccgatc atgctgttca acgcgaagct gagccaggac
     9121 atcgcgcgcg acctctacgc cgaggggatc tacgtcatcg gcttcttctt cccggtggtc
     9181 gccgcggggc aggcgcgcat ccggacgcag ctctcggccg atcacgacat cccgatgctg
     9241 gccgacgccc tcgaggcctt caagcgcgtc ggcgcgaagt acgggatcct cgggctggac
     9301 aagaagggga tcatcgagaa gtacgggctg tgacaccagt gggacaggcg tctcgcctgt
     9361 cgggtgatca ggtcacgttc aacgcagagg cgcagaggcg cggagacgac gcagagaacg
     9421 cctgaatcag gacctcttct ctgttacgtc ccggcatccc tgtgtctgtg cgtttgacgt
     9481 gggccagtga tgccgcggga cgcgcctgac agcgacgagg aaaccgatgt cgaagatcgc
     9541 gatgaagacg ccgctcgtcg agatggacgg cgacgagatg acccggatcc tgtgggcctg
     9601 ggtcaaggcg aagctgatcg agccgcacgt cgacctgaag accgagtact acgacctcgg
     9661 tctcgagcac cgcgacagga ccgacgaccg ggtcacccac gaggcggccg aggcgacgcg
     9721 ccggctgggg gtcgcggtga agtgcgccac catcacgccg aacgaagccc ggatgtcgga
     9781 atacaagctg aagaagcagt ggaagagccc gaacgccacg atccgggcgg cgctcgacgg
     9841 caccgtcttc cgcaagccca tcctcgttcc caacatcaag ccgggcgtcg tcaactggaa
     9901 gaagccgatc atcgtcggcc gccacgcgta cggggacgtg tacaagaacg ccgagatcgc
     9961 agtgcccggg ccgggcaagg gcgagctggt gttcaccggg gaggacggga ccgagaagcg
    10021 cgtgctgatc cacgagttcg gcgggccggg catcctccag gcgatccaca acaccgaggc
    10081 gtcgatccgc agcttcgcgc ggtcgtgctt cacctacgcg ctcgacgaga aggtggacgt
    10141 gtggttcgcc accaaggaca cgatctcgaa gacctacgac gcgacgttcc ggcgggtgtt
    10201 cgaggaggta ttcggacagg agttcgcgac ccggttcaag accgccggta tcgagtactt
    10261 ctacacgctg atcgacgacg tggtggcgcg ggtggtgaag ggggatggcg ggatgctgtg
    10321 ggcgtgcaag aactacgacg gcgacgtgat gtccgacatg gtcgcctcgg cgttcggcag
    10381 cctggcgatg atgacctccg tgctggtgtc gcccgacggc ttcttcgagt acgaggcggc
    10441 gcacggcacc gtgcagaagc actactacaa gcacctgaag ggcgagaaga cctccagcaa
    10501 ctcgatggcg ctcatcttcg cctggaccgg cgcgctgcgg aagcgcggcg agctcgacgg
    10561 gctgggtgac ctggcggagt tcgccacccg cctcgagcag gcctcgctcg cgacgatcgc
    10621 cgagggcgtg atgacgggcg acctggcgcg cctggcctcg ccgccgccca ggcaggtcgt
    10681 gggcagcgaa gggttcctgg acgcgatcgc cgggcggctc tgctgagacc atggatccgt
    10741 accagcagta cgccgacgac atcgtcgggc gcgcggcggc ggccgccgag gcctggcggc
    10801 gcttcgacca ggcgcaggcc gacgcggtcg tcgaggcgat ggccgcagcg gcgtgggagg
    10861 cgcgagagaa cctggcgcgg ctcgcggtgg acgagaccca gatgggggtg ttcggcgaca
    10921 aggtcctgaa gaacgcgtgg gcggcggcca tcgtgcgcga ctcgcttcgc ggcacgcgga
    10981 cggccggcgt catcgcgcag gacgaggcgt cgggcatcac cgagttcgcc gagccggtgg
    11041 gggccgtgct ggggctcgtc ccggtgacca accccacctc caccgcgatc ttcaaggcgc
    11101 tggtctgcgc caagacgcgc aacgccctca tcttcagccc gcaccgcggc gcgcggaagt
    11161 gcgtgaggga gacggcccgg atcctgagcg aagcggcgac aggggccggc gcgcccgagg
    11221 acgcgatcca ggtcgtctcg aggtcacaga ccgagtacct ccgccaggtg atggtccaca
    11281 agcggctggc gctgatcgtc gctaccggca cccgctcgat cgtcgaggcg gcgcggcact
    11341 cggggacgcc gacgctcggc gtgggaccgg gcaacgttcc cgtctacgtg cacggttcgg
    11401 ccgacctcgc cgaggcggcc cggaggatcc tgctctcgaa gacgttcgac aacggcacgg
    11461 tctgcgcgag cgagcaggcg gtcgtggtcg agtcgcccca ggcggcggcc ctgcgggaag
    11521 ctttcgagcg cggcggggcc cacttctgct cggccgccga ggtcgaggcg ctggggccgg
    11581 tgtgcttcga ggcctcgcac cgaaggatgc gcgcggacgt ggtggggcag ccggccgccg
    11641 agattgcgcg ccgcgcgggg ttccgcgtgc cggccaggac gaggctgctg gtcggcgcgc
    11701 cgggcgggat cggccccagc cacccgctct cggccgagat cctggcaccg gtgctcgcgt
    11761 ggtacgaggt gcgcagctat gccgaggcgc tgaggacctg ccgggcgatc acggcgtggg
    11821 gaggcgtcgg ccacacggtg ggtgtgcacg cgcgcgacga gtcggtggtg gccgacttcg
    11881 cgcaaatgcg cgcgtcgcgg gtcctggtca accagccgac gacacagggc gcgatcggcg
    11941 gcatcgtcaa cggcctgcag ccgtcgctga cgctctcgtg cggccccagc gcgcgcaacc
    12001 tcacgaccga caacatcggc gcccgccacc tgctcaacat ccacagggtg gtccttccgc
    12061 gggcaaaccc ggcgtgggcc gagctgacga cggggccggc ggatcgggtg tgacgggcta
    12121 tcccgccatc aagagcagcg ccgtccacgg caggatcgcg gcgcccaggt agatggccgt
    12181 cagcagcgcc acgaggcggt gaacgccggt gaggtggagg gcgatgccga gcacgcccgc
    12241 caccagcttg gcaccggtga ggccgggacc ggttccgaag gccagcatca ggctggccac
    12301 gaccgggttc ccctccatgt gggagcctag cgtggccagg ccgacgtagg tgaggacgcc
    12361 gtcgagagcc tggacggcga ggaaggcgac gacggcgacg ttgccgaagc cggaagcgcc
    12421 ggaacggttg aatatccaac gagacagcat cttaggtctg ccccacggct gacttaagct
    12481 tccaaaccca ccctctaacc cgttggataa acaacgctta tgccaaggcc acgccgcacc
    12541 tccggagcgc gtccggctgc ggctgtatgc ttttgcatcg ggctcatcca cagcgcgcaa
    12601 gccagccgcg cttaaggtcg cgggccttcc cggcaaaccc ggtctgccga gcattcttta
    12661 catggggttc tcccctcttt tcgcatttta gtgtaatttc tacacgacac cagctcgtcc
    12721 gtagcgatcg cccgccccgg ctattcgaaa gcaagtcctt gaacgataaa gacttgcatg
    12781 aatggcaagc gcgttgcctc cgtcgtgctc aggatgtttc ccctcgcgcg cgtcctgctt
    12841 ggcatggtca tggtcggtct ggcggccccg gtggctgccc aggcgcctgc ggtggcctcg
    12901 ccgagcgggg cgaccttccg gagcggcgtc gacatggtca ccgtcagcgc cgtcgtccgc
    12961 gatcggaagg gccggatcgt gaagaacctg cggcgtagcg acttcgagct gctcgacagc
    13021 ggcgcgccca aagcgatcag ggacttccgg cccgaccagg cgccgatcag cgtggccgtg
    13081 ctggtggacg gcagcggcag catgcaggtg gccgacaaga tcgccgaagc ccgggcggcc
    13141 gcgcagtacc tgttcgcgtg gctcgatccc ggccgcgacg aggtgggcat cttctcgttc
    13201 gacacggggc tttacgagtt gcggccgttc gcgacggccg acgaacccgc cgggctgcag
    13261 gcgacgctgg cgggcgtgat gccgttcggg atgacgtcgt tgcacgacgc ggtggcggcc
    13321 accgcccggg tggtggccga gcgggcgaac gtccaccgcg cggtgatcgt ggtgaccgac
    13381 ggcgtcgaca acggcagccg gctgacgccc gccgaggtgt cgggcatcgc cagctcgatc
    13441 gacgttccgg tctacatcgt cgccgtggtg tcgccgctcg atcacgcggg ggcgtcgagc
    13501 gcggtgcggt cggagcgccc ggtgccggtc ggcgatcttg ccgacctggc gcggtggacc
    13561 gggggggagc tgtacgtcag cagcagcgcc gcgcacacca gcgcggccac tcgcgagatc
    13621 gtcgaggaac tccgccatca atacctgatc gccttcgagc cgggcacccg gccgggctgg
    13681 cacccgctgg aggtgcgcac ccggaaggac aacctgatcg tccgggcgcg cagcgggtac
    13741 atggccggac aggcccagga ctagtagcgc gtggcttcag ccgcgcggcc tgggagaact
    13801 agtagcgcgt ggcttcagcc gcgcgggcag agctgggagc gcgcggcttc agccgtgcag
    13861 tcgttcaccg ggtttcatcg aggaggcgtc cgatcatgag gaagttcgtg ctggccatgt
    13921 cggtggcatc gatcgcggcc atgggctcca cggcgtgcgc gacgaaggga ttcgtccgcg
    13981 accgcgtggg cgaggtcaac gacaaggtgg agaccgtgtc gcgctcgctg gaggagaccc
    14041 aggagcggac gaggaagaac gaggctcgca tcggcgaggc cgagcagaag atccagcagg
    14101 tggatcagcg cgcggcggcc gccaacacgg cggcgaccga ggcgcggacg gcggccgaca
    14161 ccgcggccaa caaggccgag gcggtggaga agtcggtcaa gcggatcgtc tacgaggtga
    14221 ccctgagcga ggaccagggc aacttcaagt tcggccaggc ggcgctcccc gacgaggcga
    14281 aggcgcgcct cgacgaggtg gcctcgcagc tgaaggcgaa cccgaacggc gcgttcatcg
    14341 aggtcgaggg ctacaccgac agcgtcggct cgaaggagta caaccagcag ctgggcctcc
    14401 agcgcgccga gtcggtgaag cggtacctct actcgcagca ccagatcccg ttgcaccgga
    14461 tcaacgtgat cagctacggg gccgagaacc cgattgcccc gaacaacacc cgcgcgggcc
    14521 gcgcgaagaa ccgccgggtg gtgatcaagg tcctggtcta gggactgggg gcgttccagc
    14581 ttgcggtgcg ggcgagacaa cgcgcccggt gctgaggagc ggcttccgca tcgcaggaca
    14641 aggccggcgc cggaacatgg gatcctggcg tcggcctctt cttccgttct ccgttacccc
    14701 ccgcgttcgg ccttgcagta tcctccctca atggccatac gcaccgaact gacgctccgg
    14761 ctgcagaaca gccccggcat gctcgcccgg gtttgccagg tgatcgccaa cgagcggatc
    14821 aacatcgtgg cgatgaacct ggcgagcccc ctcgtcgtgc gcctggtcgt cgacaatccc
    14881 gtccacgccg ctgccgtcct ccgcgagcac cagtaccagg tcgaggagcg cgacgtcatc
    14941 tacacggcga cgtcgaacga cccgggcgcg ctgctccgga tcacgcggct catcgccgac
    15001 gcgggcatca acgtcgagta cctctacgcc accgccctgg aggggcagtc gatggccagc
    15061 gtggtcgtgg gcgtccccga tgccgagcgt gcctcgtcgg cggcgggcct gtagccgcct
    15121 gggatgaacg actcgcctgc ctccggcccg gcgacatcac ggccccggcg gcggcccggc
    15181 cgcctgctcg tgccgctcaa gaccctgcgc gtccgcctcg cgctgctggt caccctcgtc
    15241 gtcgccctcg tcgtcggggc cagcagctac ctgcagatgc gggtgttcga gtcggagatc
    15301 agcgccggcc tcgaggacac ggcaatcaag attgccgtcg ccgtggccga cgacgtcgag
    15361 gtgcggcccg agccgttgaa ccacggggcg ctcgccgaga gcctgcgcga gttctacgag
    15421 gcggtgccgt cgctgcgcgc g
//
LOCUS       DAOPQX010000048        15215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4631_length_15215_cov_4.944723, whole genome shotgun sequence.
ACCESSION   DAOPQX010000048 DAOPQX010000000
VERSION     DAOPQX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15215
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4631_length_15215_cov_4.944723"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2013)
                     /locus_tag="PKK95_04330"
     CDS             complement(<1..2013)
                     /locus_tag="PKK95_04330"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOC17470.1"
                     /translation="MMTARPSRFLPILTFLLAVAAVPGEAQSPAANAPGATVREYKKV
                     FRTYPYSDPNAIPVVGRIYPYYRFDGYTNAPVDREWTVVELENAYLKVWVLPEIGGKI
                     WAAFEKSTGKSFLYNNRVVKFRDIAMRGPWTSGGIEANYGIIGHTPNCATPVDYQVRT
                     SADGGASVTVGTLDLLTRTVWRIEVGLPADRAYFTTRSYWYNGSALEQPYYTWMNGAI
                     KAAGNLELVYPGTHHIGHGGEASAWPIDPATGRNLAFYEQNDFGPAKSYHVVGRHEGF
                     FGGYWHDEDFGMARYAGRDEKLGKKAWIWGLSRNGMIWEDLLTDEDGQYVEVQSGRLF
                     NQAAEDSTRTPFKHTGFAPHAADAWTEYWFPVKGTGGFVKADRHGALNVTIEKGRLRV
                     AFSPTEAIEGPVEIKDGERVLFAGRVSLKPMQTWVQAVEGAKSADRVSVRIGDGAFEY
                     EAAAETALSRPVASPADFNWESAFGAWTSGKEWIRQREYARARSALEASLAKDPHFVP
                     ALGDLAMLDLRERRDRAAWDRARLALSVDTYDPLANYVYGLASRRLGKTADAKDGFEV
                     AVLSPAWRAAAWTELSKIYLSEGRLREAEEYAVKAAGSEPTSLDPWQLRATIARLRND
                     SASARTHLDRLASIDGLNHFQRFERHLLAPQDESARRDFLSGVRNEM"
     gene            2068..5337
                     /locus_tag="PKK95_04335"
     CDS             2068..5337
                     /locus_tag="PKK95_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOC17471.1"
                     /translation="MKTTLLRLTITLLALAGLATTAPAQPAAGWLRYPAISPDGQTIV
                     FTYRGDLYRVPAAGGHAVPLTLHDAHDFMPVWSRDGRQIAFASDRHGNFDVFVIPAGG
                     GEARRLTFHSADEFPYAFEPGDRAVVFGSARLDTAANRGYPTASQPELYRVPAGGGRV
                     EQILTTPAEDVSIDATGTLFLYHDKKGGENAWRKHHTSAIARDIWAFDTKAGTHRKIT
                     TFEGEDRTPVLADQGKAFYYLSEESGSFNVHKMALQGGRSQQLTSFKKAPVRFLTSSA
                     SGLLCFGHDGEIYTMKPGAQPQKVAVALAVDARANARRVLPVTGGVRELAVAPSGKEV
                     AFVFRGEVFVTSVDGGVTKRITDTPGQERGVSFSPDGKALLYASERGDRWRIVEARRV
                     RDEEPYFYASTLIRETPIVANGKENYQPAYSPDGKEIAYIEDRVTLRVYNTESKQTRT
                     LLTDRHLFSNGDADQYFQWSPDGRWILFDYAVPGFAPGEVGLVRADGTGEVINLTRSG
                     FHDRGARWILGGKGMLWLSNRDGLRAAAQSGGAQLDAYAMFFTQEAWDRFRLTKEELA
                     LVKEIEEKREKEKKEKAEAAKDEEKKKDAKDKEAKPEEVVIDLDGIEVRKARLTIHSS
                     SLGDALVSKDGDTLYYLARFEKGMNLWSTNLRTRETRMVLALAANAARLEWDKEQKNL
                     FLLADGSLSKVDPATAKKEPISIKGEMVLDVPAEREFMFEHVWRRTARTFYTAGYHGA
                     DWPALKTMYQKHLAHVGNGHEFAELLSEMLGELNVSHSGARYARSAPEDDATAALGVF
                     YDPAHRGAGYRIAEVVKDGPLDRAGVKPAPGTVIEAIDGEQVAANRDLAEFLNRKAGR
                     NVLLTLVDPSGKRDVVVKPITLAEEGRLLYTRWVRRNQEEVDRVSKGRLGYIHVPGMN
                     DGAYRSVFEEALGKYAPREGLVVDTRFNGGGDLVADLAMFLSGRKFFDYTTDTRSNGY
                     EPNFRWTKPSVSLANEANYSDGHCYAYTVKALGLGKLVGMPVPGTCTFAGWEMLPDGS
                     TRWGVPPLGVKNVEGQYLENRQTVPDIAVANSAERQAAGQDEQLEAAVAALLAREENN
                     R"
     gene            5386..5916
                     /locus_tag="PKK95_04340"
     CDS             5386..5916
                     /locus_tag="PKK95_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOC17472.1"
                     /translation="MRRPRVLAAAMVLAVSVTGCRSLEGLGQLIRPPRFEQAPGQPAE
                     IAFSGVSAAMPAGGATVRIWTKVTNPNPFGFTLSTLSTTLLLDGSRAATGDFPLGLPL
                     EASQSAVVPIELTISFSDVPALTSVARSALTGLGVQYRLDGTVGVDAGRLGQPTFGPL
                     MLLEGELRARRIVGSK"
     gene            complement(5951..6337)
                     /locus_tag="PKK95_04345"
     CDS             complement(5951..6337)
                     /locus_tag="PKK95_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17473.1"
                     /translation="MPDYVERLASTRLALLDLHKLLLTRERRAYEREHGPVSAGSLLE
                     LAINDGRFGWLHRISEIVVRIDEMLEWRDLWTDADAEWVLAGTRALLVPSPEGTDFEQ
                     RYDAALQDDPHIVLAHRAVIRAAKGT"
     gene            complement(6348..7517)
                     /locus_tag="PKK95_04350"
     CDS             complement(6348..7517)
                     /locus_tag="PKK95_04350"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HOC17474.1"
                     /translation="MKATRWQGKQHLAVEEVPDPAIMNPRDAIVRITSTAICGSDLHL
                     YNGFIPSMRKGDILGHEFMGEVEEVGPEVRGLRAGDRVVVPFPIACGACLQCQRGLFS
                     LCENSNPNAWMAEKLWGFSPAGIFGYSHMLGGYAGGQAEFARVPYADVNHVKVPDSLA
                     DEQVLFLSDILPTGYMAAENCGISPGDTVAVWGCGPVGQFAILSAYLLGAGRVIAIDR
                     FPYRLEMAHDHGKADVLNYEEVDVHEALKEMTGGRGPDSCIDAVGMEGHTPGPVGAYD
                     RVKQAAMLETDRPVALRQAILACRGGGTISVAGVYGGFIDKFPMGAIVNRSLTIKSGQ
                     THVQRYMRPLLQRIERGEIDPTFIITHTMRLEDAPAGYDVFLNKEDECVKVVLKP"
     gene            complement(7514..8512)
                     /locus_tag="PKK95_04355"
     CDS             complement(7514..8512)
                     /locus_tag="PKK95_04355"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOC17475.1"
                     /translation="MTQPEREGEPARAEGPAAAKLSPAARRNGGNGRLADGLGWFSLA
                     LGGAEVAVPALVARNIGVPNTPENRLLLRVLGAREIASGLGILGRDRQAPWVWSRVAG
                     DAMDLVLLGLALGSATSKRRQLGVATAAVAGVAALDVVAGKRQARHDASRAGFEHVTR
                     SMTVNKPPAEVYGFWRNLENLPRFMKHLRSVTVTGPDRSHWVVQGPAGRSVEWDAEIV
                     EERPGEFLAWRSLPGSQVDHSGWVSFRPAPGGRGTEVSLRLAYHPPAGRLGSAVAWLF
                     GREPEQQLREDVRRFKQVIETGEIARSDASRGLLGMRRAQPGTAQPYPAFERGEER"
     gene            8630..9754
                     /locus_tag="PKK95_04360"
     CDS             8630..9754
                     /locus_tag="PKK95_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOC17476.1"
                     /translation="MERRVRTAAWMAVILLAVAGLALTVRGIVAPRLNAQPVVPDIAP
                     RGALLPGEQATVALFEKTRASVVFITTEQQVMDVWTRNIFNIPRGSGSGFAWDDKGYL
                     VTNNHVVAGASAASVRLSDGRDVPAKLVGASPAHDLAVLKIDVAKAPPALPIGTSHDL
                     RVGQATFAIGNPFGLDWTLTSGIVSALDRSLPSEDNRLLIEHLIQTDAAINPGNSGGP
                     LLDSAGRLIGVNTAIFSPSGASAGVGFAVPVDTVRRVVPQLVATGRYVRPALGIQVDE
                     RLNELITEQLGVKGVAVLRVTPGSPADRAGLKGVRSSQGGTNITPGDIIVAVDGTPVE
                     SVARLLGRLDEHEVGQQVRLSVMREGKKVEVDVTLEAGGQ"
     gene            9840..10577
                     /locus_tag="PKK95_04365"
     CDS             9840..10577
                     /locus_tag="PKK95_04365"
                     /inference="COORDINATES: protein motif:HMM:NF025013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HOC17477.1"
                     /translation="MSWRQRALGVVVAASLTMVGATAAAQSTAPTPGSGQSATGAHGQ
                     DTRSPASRQDDSSKFYKKAAMGNMAEIELGKLGARKAQDPQVKQFAQQMVDAHQRALD
                     NLKEVAAGNVEWPATLDEKHRKKLDQLAELSAEEFDREFMEVMIAAHESIEDHLEDYT
                     GENRRGAGDATAGTSGTGGATGSGSSAGAGSSTGGAGEAAAGTSGVERTGEPRLSGAA
                     YDWASNSLREVRSHLEQAKEIKERLEK"
     gene            complement(10555..12531)
                     /gene="ftsH"
                     /locus_tag="PKK95_04370"
     CDS             complement(10555..12531)
                     /gene="ftsH"
                     /locus_tag="PKK95_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOC17478.1"
                     /translation="MTEPQPKPEPQKQATSPQPGRKPDEPATKPWRTEGLPKGEQPPP
                     SRRRWTWMAAFFVGYLVLFGLLTVQDRMSGPQAISYTEFKAQVSRKNVKEVFARGDTI
                     EGQLKEPAELPGEQGHTYQQFTTERPTFAADDLLAELAAGGASVRATPLVEHRGLLAN
                     LLISMLPILLLVGFWVWMFRRQRASMGGGLLGGRPRKPVDPETVRVTFDDVAGIDEVE
                     AEISEIVDFLRDPGKYRRVGARAPKGVLLTGAPGTGKTLLARATAGEAKVPFFSASAS
                     EFIEMIVGVGASRVRELFNEARKVAPAIIFIDEIDTIGRTRAGARSLGGHDEREQTLN
                     QILTEMDGFSGTEGVVVLAATNRADVLDPALLRPGRFDRTITVNAPDQKGRAEILKVH
                     TRKVPLAPDVSLEAVASSTPGMTGAELANLVNEAALQAARQDRDTVTQRDLFDALEKI
                     QLGTARKVVISEPERRRTAYHEAGHALIGMLQPGADPVRKVSIIPRGRALGVTLSTPE
                     ADRYGYDANYLRGRIIGALGGMAAEQEVFGVVTTGAESDLEMVTRVARGMVGRWGMSE
                     RVGKISVYPPDGDPRMMGTSDALLDAVDEEVRRFIDECYDEARRLLRENRDKLDAIVE
                     QLLARETLDEREVYAAAGLAAAARSADATFRDAP"
     gene            complement(12600..13235)
                     /locus_tag="PKK95_04375"
     CDS             complement(12600..13235)
                     /locus_tag="PKK95_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC17479.1"
                     /translation="MNAADPHGSQPVLRRGPDPAAARLCAILLHGRGSTASDILSLAD
                     ELDLADVLYLAPDAAGHTWYPYSFLQPLDRNQPHLDSALALVDRAVARLGAAGVPPAR
                     VALLGFSQGACLALEYAARHARRYAAVVGLSGGVIGPPGTPRAYEGSMEATPVFLGCS
                     NVDPHIPVERVHETAGVFRTLGALVDERIYPGMGHVVNDEEIQAVRELLLG"
     gene            complement(13246..13707)
                     /locus_tag="PKK95_04380"
     CDS             complement(13246..13707)
                     /locus_tag="PKK95_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HOC17480.1"
                     /translation="MDRTRLLARLDEKWRDFTGSFAGLSDEELTAPGVTRAWSVRDII
                     AHVTTWEEEALKYLPVILEGKRPPRYSTLYGGIDAFNAKTTAEKAALPLAEVLRRLDG
                     THRRLVRFVEQAPDEALAAESRFVRRLRADTYGHYPRHAEAIRKWRAARQR"
     gene            complement(13794..14822)
                     /locus_tag="PKK95_04385"
     CDS             complement(13794..14822)
                     /locus_tag="PKK95_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOC17481.1"
                     /translation="MIKTVCFGEIMLRLSPPGFERLFQSPLLHATFGGGEANVAVSLA
                     HFGLDSHYVTRLPANPVGDAALRALRAEGVRVEPVLRGGSRLGIYFAETGASQRPSLV
                     VYDRAHSAIGELQPGAIPWEAVLDGAAWFHVTGITPALGPGPAACTAEALAAAAKAGV
                     RVSLDLNYRAKLWSKADAQRTMRALMPYVDVAIANEEDLQASLGIEVARADVKAGVLD
                     AEAYRAAAGQVVREFGVKQVAVTLRESLSASDNAWSAVLFDAATGAFHQAQRYVVRLV
                     DRIGGGDSFAAGLVYSLAAGRDAASALRFAVAASAIKQTIPGDFNRVSVAEVERLAGG
                     DASGRVQR"
     gene            complement(14825..>15215)
                     /locus_tag="PKK95_04390"
     CDS             complement(14825..>15215)
                     /locus_tag="PKK95_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOC17482.1"
                     /translation="FVVSPVFRPAVLEVARLHDVAVMPGCFTPTEILDAWDAGADLVK
                     VFPATVLGPAFFKDMRGPLPHVRLMPTGGVTRDNAGEWIAAGAAAIGVGTALVDRAAI
                     KAGQFDRIAANAAHFIEAVRRARGQGR"
BASE COUNT         2295 a         5437 c         5316 g         2167 t
ORIGIN      
        1 catctcgttc ctcacgccgg acaggaagtc gcgcctggcc gactcgtcct gcggcgccag
       61 gaggtgacgc tcgaatcgct ggaagtggtt caggccgtcg atcgacgcca ggcggtccag
      121 gtgggtgcgg gccgacgcgg aatcgttgcg cagcctggca atcgtcgcgc gcagctgcca
      181 ggggtcgagc gacgtcggct ccgacccggc agccttgact gcatactctt cggcctcgcg
      241 gaggcggcct tccgagaggt agatcttgga caactcagtc catgccgcgg cccgccaggc
      301 cggggagagg acggccacct cgaagccgtc tttcgcgtcg gccgtcttgc cgagccgccg
      361 cgacgccagg ccgtagacgt agttggccag cgggtcgtac gtgtcgacgc tcagtgccag
      421 gcgcgcccgg tcccacgccg cccggtcacg ccgctctcgt agatcgagca tcgcgaggtc
      481 gccaagcgcc ggcacgaagt gcgggtcctt cgcgaggctg gcctcgagcg cgctccgggc
      541 gcgcgcgtac tcgcgctgcc ggatccactc cttgcccgac gtccacgccc cgaacgccga
      601 ctcccagttg aaatcggccg gcgaggcgac cgggcgcgaa agggcggtct cggcggccgc
      661 ctcgtactcg aacgccccgt cgccaatccg caccgacacg cggtcggcgc tcttcgctcc
      721 ctccacggcc tggacccagg tctgcatcgg cttcagcgac acgcggcccg cgaagagcac
      781 gcgctcgccg tccttgatct cgaccggacc ctcgatggcc tcggtcggtg agaacgccac
      841 ccgcagccgc cccttctcga tcgtgacgtt cagcgcgccg tgccgatcgg ccttgacgaa
      901 cccgcccgtc ccctttaccg ggaaccagta ctcggtccac gcgtccgccg cgtgcggggc
      961 gaacccggtg tgcttgaagg gcgtccgcgt gctgtcctcg gcggcctggt tgaacagccg
     1021 gcccgactgc acttcgacgt actggccgtc ttcgtcggtg agcaggtcct cccagatcat
     1081 cccgttccgc gacagccccc agatccacgc cttcttcccg agcttctcgt cccgcccggc
     1141 gtaccgcgcc atgccgaagt cctcgtcgtg ccagtacccg ccgaagaacc cctcgtgacg
     1201 gccgacgacg tggtacgact tggcggggcc gaagtcgttc tgctcgtaga aggcgaggtt
     1261 gcggccggtt gcgggatcga tcggccaggc cgaggcctcg ccgccgtgcc cgatgtggtg
     1321 cgtgccgggg tagaccagct cgaggttgcc ggccgccttg atggcgccgt tcatccaggt
     1381 gtagtaaggc tgctcgagcg ccgagccgtt gtaccaatac gaccgcgtgg tgaagtacgc
     1441 gcggtcagcc ggcagcccca cctcgatcct ccagacagtg cgcgtcagca ggtcgagcgt
     1501 tccgacggtc acgctcgcgc cgccgtcggc cgaggtgcgc acctggtagt ccaccggcgt
     1561 ggcgcagttc ggcgtgtggc cgatgatgcc gtagttggcc tcgatgccgc cgctcgtcca
     1621 cggcccgcgc atcgcgatgt cgcggaactt caccacccgg ttgttgtaga ggaacgactt
     1681 ccccgtcgac ttctcgaacg ccgcccagat cttcccgcca atctccggaa gcacccacac
     1741 cttcaggtac gcgttctcca gctcgacgac ggtccactcc cggtcgacgg gcgcgttggt
     1801 gtacccgtcg aagcggtaat aggggtagat gcgcccgacc accgggatcg cgttggggtc
     1861 ggagtatggg tacgtgcgga agaccttctt gtactcgcgg accgtggcgc ccggggcgtt
     1921 cgcggccggc gactgggcct cgccgggcac ggccgcgact gccagaagaa aggtcaggat
     1981 cggcagaaat ctggacgggc gagcggtcat catggcgggg attgtgccac aatcccctgt
     2041 ccccgcaacg cgccctcaca ccacaccatg aagaccacac tgctccgact cacgattacc
     2101 ctgctcgcgc tcgccggatt ggcgacaacc gcccctgccc agcccgcggc gggctggctg
     2161 cgatacccgg ccatctcgcc cgacggccag accatcgtct tcacctatcg cggcgatctc
     2221 taccgcgtgc cggccgccgg cggtcacgcc gttccgctca ccctccacga cgcgcacgac
     2281 ttcatgcccg tctggagccg cgacggcagg caaatcgcct tcgccagcga ccggcacggc
     2341 aacttcgacg tcttcgtcat ccccgccggg ggaggcgagg cgcggcggct cacgttccac
     2401 tcggcagacg agttccccta cgccttcgag ccgggcgacc gcgccgtggt cttcggctcg
     2461 gcccgcctcg acacggcggc caaccgcggc taccccaccg cgtcgcagcc cgagctgtac
     2521 cgcgtgcccg ccgggggcgg ccgcgtcgag cagattctga cgacgccggc cgaggacgtg
     2581 agcatcgacg ccaccggtac gctcttcctg taccacgaca agaagggggg cgagaacgcc
     2641 tggcgcaagc accacacctc ggcgatcgcc cgcgacatct gggccttcga cacgaaggcc
     2701 gggacgcacc gcaagatcac gaccttcgag ggcgaggacc ggacgccggt gctggccgac
     2761 cagggcaagg cgttctacta cctcagcgag gagagcggct cgttcaacgt ccacaagatg
     2821 gccctgcagg gcgggcggtc gcagcaactc acctccttca agaaggcgcc ggtccgcttc
     2881 ctcacctcct cggcctcggg gctgttgtgc ttcggccacg acggcgagat ttacacgatg
     2941 aagccgggtg cccagccgca gaaggtcgcg gtggccctcg ccgtggacgc gcgcgccaac
     3001 gcgcgccggg tgctgccggt gacgggcggc gtgcgcgagc tggccgtcgc gccgagcggc
     3061 aaggaagtgg ccttcgtctt ccgcggcgag gtgttcgtca ccagcgtgga cgggggcgtg
     3121 accaagcgca tcaccgacac gcccggccag gagcgcggcg tgagcttctc gcccgacggc
     3181 aaggccctgc tctacgcctc ggagcgcggc gaccgctgga ggatcgtcga ggcgcggcgc
     3241 gtgcgcgacg aggagccgta cttctacgcc tcgacgctga tccgcgagac gccgatcgtc
     3301 gccaacggca aggagaacta ccagccggcc tactcgcccg acggcaagga gatcgcctac
     3361 atcgaggacc gggtgacgct gcgtgtctac aacacggagt cgaagcagac gcgcaccctc
     3421 ctcaccgaca ggcacctctt ctcgaacggc gacgccgacc agtacttcca gtggagcccc
     3481 gacgggcgat ggatcctctt cgactacgcg gtgccgggct tcgccccggg cgaggtcggg
     3541 ctcgttcgcg ccgacggcac cggcgaggtg atcaacctca cccgcagcgg cttccacgac
     3601 cggggggcgc gctggatcct cggcggcaag gggatgctgt ggctcagcaa tcgcgacggc
     3661 ctgcgcgccg cggcccagag cggcggcgcg cagctcgacg cctacgcgat gttcttcacg
     3721 caggaggcgt gggaccggtt ccgtctcacc aaggaggagc tggccctggt gaaggaaatc
     3781 gaggagaaga gggagaagga aaagaaggag aaggctgagg cggcgaagga cgaggagaag
     3841 aagaaggacg ccaaggacaa ggaggcgaag cccgaagagg tcgtcatcga cctcgacggg
     3901 atcgaggtcc gcaaggcccg cctcaccatc cactcctcct cgctcggcga cgcgctcgtc
     3961 agcaaggatg gcgacaccct ctattacctc gcccgcttcg agaagggcat gaacctctgg
     4021 tccacgaacc tgcggaccag ggagaccagg atggtgctcg cgctggccgc caacgccgcc
     4081 cgcctcgagt gggacaagga gcagaagaac ctcttcctgc tcgccgacgg cagcctctcg
     4141 aaggtcgacc cggcaaccgc gaagaaggag ccgatctcga tcaagggcga gatggtgctc
     4201 gacgtccccg ccgagcgcga gttcatgttc gagcacgtct ggcggcgcac cgcgcgcacc
     4261 ttctacaccg cggggtacca cggcgccgac tggccggcgc tcaagacgat gtaccagaag
     4321 cacctggcgc acgtcggcaa cggccacgag ttcgccgagc tgctgagcga gatgctgggc
     4381 gagctgaacg tgagccacag cggcgcccgc tacgcccgct cggcccccga ggacgacgcg
     4441 accgccgcgc tcggcgtctt ctacgacccg gctcaccggg gcgcgggcta ccgcatcgcc
     4501 gaggtggtga aggacggccc gctcgaccgc gcgggcgtca agcccgcacc cggcacggtg
     4561 atcgaggcga tcgacggcga gcaggtcgcg gccaaccgcg acctcgccga gttcctcaac
     4621 cggaaggcgg ggcgcaacgt cctgctcacc ctcgtcgatc cgtccggcaa gcgcgacgtc
     4681 gtggtcaagc cgatcacgct cgccgaggaa ggacggctgc tctacacgcg gtgggtacgg
     4741 cgcaaccagg aggaggtgga ccgggtcagc aaggggcggc tcggctacat ccacgtcccc
     4801 ggcatgaacg acggcgccta ccgctcggtc ttcgaggagg cgctcggcaa gtacgccccg
     4861 cgcgagggac tggtggtgga cacgcggttc aacggcgggg gcgacctggt cgccgacctg
     4921 gcgatgttcc tcagcggccg gaagttcttc gactacacca ccgacacgcg aagcaacggg
     4981 tacgagccga acttcaggtg gaccaagccg agcgtgtcgc tggccaacga ggcgaactac
     5041 agcgacgggc actgctacgc ctacacggtg aaggcactcg gcctcgggaa gctggtcggg
     5101 atgcccgtgc cgggcacgtg cacgttcgcc gggtgggaga tgctgcccga cggaagcacc
     5161 cgctggggcg tccccccgct cggggtgaag aacgtggaag ggcagtacct cgagaaccgc
     5221 cagaccgtgc ccgacatcgc ggtcgccaac tcggccgaac gccaggccgc cggccaggac
     5281 gaacagctcg aggccgccgt ggcggccctg ctcgcccgtg aggagaacaa ccggtaggaa
     5341 ggaagcgggg agaagctcgg ccgggacggt gctacgatgc tcagaatgag acgaccgcgc
     5401 gtcctggccg cggccatggt cctggccgtc agtgtcacgg gctgccgttc gctggagggg
     5461 ttggggcagc tcatccggcc gccccgcttc gagcaggcgc cggggcagcc cgccgagatc
     5521 gccttcagcg gcgtctcggc ggccatgccc gccggcggcg ccaccgtgcg catctggacc
     5581 aaggtcacca acccgaaccc gttcggcttc acgctcagca cgctgagcac gaccctgctg
     5641 ctcgacggct cgagagccgc gacgggagac ttcccgctcg gcctgccgct cgaggcgtcg
     5701 cagagcgccg tcgtgccgat cgagctgacg atcagcttct cagacgtgcc ggcgctgacc
     5761 tccgtggcgc gctcggcgct caccgggctg ggcgttcagt accggctcga cggcaccgtc
     5821 ggggtggacg ccggccgcct cggccagcct accttcggtc cgctgatgct gctcgaaggc
     5881 gagctgcgcg cgcggcgcat cgtcgggtcg aagtgagtgg gacgacccgg gcgcccacaa
     5941 ggggcgcccc ttacgtgccc ttcgccgccc tgatcacggc gcggtgggcc aggacgatgt
     6001 gggggtcgtc ctggagggcg gcgtcgtagc gctgctcgaa gtcggtgccc tcgggcgaag
     6061 gcaccagcag cgcgcgagtt cccgcgagca cccactcggc gtcggcgtcg gtccacaggt
     6121 cgcgccactc cagcatctcg tcgatccgca cgacgatctc gctgatccgg tgcagccagc
     6181 cgaagcggcc gtcgttgatg gccagctcga gcaggctgcc tgcgctcacc ggtccgtgct
     6241 cgcgctcgta cgcccggcgc tcgcgcgtca gcaggagctt gtgcagatcg agcagcgcca
     6301 ggcgcgtgga cgcgaggcgt tcgacgtagt cgggcatggt gatgacgtca gggcttgagc
     6361 accaccttga cgcactcgtc ctccttgttc aggaagacat cgtaccccgc cggcgcgtcc
     6421 tcgaggcgca tcgtgtgggt gatgatgaag gtggggtcga tttccccccg ctcgatccgc
     6481 tggagcagcg gcctcatgta gcgctgcacg tgcgtctggc ccgacttgat ggtcagcgac
     6541 cggttgacga tcgcccccat ggggaacttg tcgatgaacc cgccatagac gcccgcgacc
     6601 gagatcgttc ccccaccgcg gcacgcgagg atcgcctgcc gcagcgcgac gggccggtcg
     6661 gtctcgagca tggccgcctg cttcacccgg tcgtacgccc ccacggggcc cggggtgtgc
     6721 ccctccatcc cgaccgcgtc gatgcagctg tcggggccgc ggccgccggt catctccttc
     6781 agcgcctcgt gcacgtcgac ctcctcgtag ttgaggacgt cggccttccc gtggtcgtgc
     6841 gccatctcga gccggtacgg gaagcgatcg atcgcgatca cccgcccggc gccgagcagg
     6901 taggcgctga ggatggcgaa ctgccccacc gggccgcagc cccagacggc caccgtgtcg
     6961 ccagggctga tgccgcagtt ctcggcggcc atgtagccgg tgggcaggat gtccgagagg
     7021 aacagcacct gctcgtcggc cagggagtcg ggcaccttca cgtggttgac gtcggcgtag
     7081 ggaacgcgcg cgaattcggc ctgcccgccg gcgtacccgc cgagcatgtg cgagtagccg
     7141 aagatgcccg ccggcgagaa tccccacagc ttctccgcca tccacgcgtt cgggttgctg
     7201 ttctcgcaga gcgagaagag accccgctgg cactggaggc aggcgccgca cgcgatcggg
     7261 aacgggacga ccacccggtc gccggcgcgc aggccgcgca cctccggccc gacctcctcg
     7321 acctcgccca tgaactcgtg cccgaggatg tcccccttcc gcatggaggg gatgaacccg
     7381 ttgtacaggt gcaggtccga cccgcagatc gccgtcgagg tgatccggac gatcgcgtcg
     7441 cgcgggttca tgatggccgg gtcgggaacc tcctcgaccg ccaggtgctg cttgccctgc
     7501 cagcgcgtcg ccttcatcgc tcctctcccc tctcgaacgc cgggtagggc tgcgccgtgc
     7561 cgggctgcgc gcggcgcatg ccgagcagcc cgcggctcgc gtccgaccgc gcgatctcgc
     7621 cggtctcgat cacctgcttg aagcgccgca cgtcctcgcg cagctgctgt tcgggctcgc
     7681 ggccgaacag ccacgcgacg gccgaaccca gccggcccgc cggcgggtgg taggccaggc
     7741 gaaggctcac ctcggtgccg cggccgcccg gagcgggccg gaagctgacc cagcccgagt
     7801 gatccacctg cgagccgggc agcgaccgcc acgccaggaa ctcgccgggc cgctcctcga
     7861 cgatctcggc atcccactcg acgctgcggc cggccggccc ctgcaccacc cagtggctgc
     7921 gatcgggccc ggtcacggtc accgaccgga ggtgcttcat gaagcgcggc aggttctcga
     7981 ggttcctcca gaacccgtag acctcggccg gaggcttgtt gacggtcatc gagcgcgtga
     8041 cgtgttcgaa accggcgcgg ctggcgtcgt ggcgtgcctg gcgcttcccg gccacgacgt
     8101 cgagcgcggc gacgccggcc acggccgcgg tagcgacgcc gagctggcgg cgcttcgacg
     8161 tggccgaacc caaggcgagg cccagcagca ccaggtccat cgcatcgccc gccacccgcg
     8221 accagaccca gggagcctgg cggtcgcgcc ccaggatgcc gaggccgctg gcgatctcgc
     8281 gcgcgccgag cacgcgaagc aggagccggt tctccggcgt gttcggcacc ccgatgttcc
     8341 gggcgaccag ggccggcacg gccacctcgg ccccgccgag cgcaaggctg aaccacccga
     8401 gcccgtcggc cagccggccg ttcccgccgt tgcgcctggc agccggcgac agcttcgcgg
     8461 ccgcgggccc ttccgcccga gccggctctc cttcccgttc cggttgtgtc atctgacctc
     8521 ccgcgcggca ccgtgcaatg gacgatccgg ccggctcgag ctggaccgcg gcaccttcgt
     8581 tgcagcgcct gtcaccctgc agtcgtgcct cccgggagga agagaagcca tggaacgtcg
     8641 agttcgcacc gccgcgtgga tggccgtgat cctgctggct gtcgccggcc tggccctgac
     8701 cgtccgcggg atcgtcgcgc cgcgcctcaa cgcgcagccg gtcgtccccg acatcgcgcc
     8761 gcgcggcgcc ctgctgccgg gcgagcaggc gaccgttgcc ctcttcgaga agacgcgcgc
     8821 ctcggtggtg ttcatcacga ccgaacagca ggtgatggac gtctggacga ggaacatctt
     8881 caacatcccg cgcggcagcg ggtccgggtt cgcctgggac gacaaggggt acctcgtgac
     8941 gaacaaccac gtggttgccg gagcctcggc ggcgagcgtg aggctgagcg acgggcgcga
     9001 cgtgccggca aagctcgtgg gcgccagccc cgcgcacgac ctcgcagtgc tgaagatcga
     9061 cgtggcgaag gcgccgccgg cgctgccgat cggcacgagc cacgacctgc gcgtgggcca
     9121 ggcgaccttc gcgatcggca acccgttcgg cctcgactgg acgctgacgt ccggaatcgt
     9181 gtcggccctc gaccggtcgc tgccatccga ggacaaccgc ctcctgatcg agcacctgat
     9241 ccagaccgac gccgccatca acccgggcaa ctcggggggg cccctcctcg acagcgccgg
     9301 gcgcctcatc ggcgtcaaca ccgccatctt cagtccctcg ggagcgtcgg ccggggtcgg
     9361 ctttgcggtg ccggttgaca cggtccgtcg cgtggtgccc cagctcgtcg cgaccggacg
     9421 ttacgtgcgg cccgcgctcg gcatccaggt ggacgagcgc ttgaacgagc tgatcaccga
     9481 gcagctcggg gtcaaggggg tggccgtgct ccgcgtgacg ccgggctcgc ccgccgaccg
     9541 cgccgggctg aaaggagtcc gctcctcgca aggcggcacg aacatcactc caggcgacat
     9601 catcgtcgcg gtcgacggga cgccggtcga atcggtcgca cgcctcctgg gccggctcga
     9661 cgagcacgag gtcgggcaac aggtccggct gtcggtcatg cgtgaaggaa agaaggtcga
     9721 ggtggacgtc acgctcgagg cgggcgggca gtaggaaggc tcacggctga gggttcacgg
     9781 ctcaagcagg tacgaggctt gccagccatc ccgccgaaca gcagtggagg gctgaactga
     9841 tgagttggag acagcgggca ttgggcgtcg tggtggcggc gtcgctcacc atggtgggcg
     9901 caacggcggc ggcacagagc acggcgccca ctccgggcag cggtcagagc gccaccggcg
     9961 cgcatggcca ggacacgcgg agcccggcgt cgcggcagga cgactcgtcc aagttctaca
    10021 agaaggctgc gatgggcaac atggccgaga tcgagctcgg caagctgggc gcccggaagg
    10081 cgcaggaccc gcaggtgaag cagttcgcgc agcagatggt ggacgcccac cagcgagcgc
    10141 tcgacaactt gaaggaggtc gccgcgggga acgtcgagtg gcccgccacg ctcgacgaga
    10201 agcaccggaa gaagctggac cagctggctg aactcagcgc cgaggagttc gaccgtgagt
    10261 tcatggaggt gatgatcgcc gcccacgagt cgatcgagga tcatctcgag gactacaccg
    10321 gggagaaccg gcgaggcgcc ggcgatgcta cggccgggac ctcgggcacg ggcggcgcca
    10381 ccggcagcgg ctcctcggca ggtgccggca gctctactgg cggggcgggc gaggccgcgg
    10441 ccggcacgtc gggcgtcgag cggacgggcg agccccggtt gtcgggcgcg gcgtacgact
    10501 gggcgtcgaa ttcgctgcgg gaggttcgct cgcacctcga acaggcgaag gagatcaagg
    10561 agcgtctcga aaagtagcgt cggcgctccg cgcggcggcc gccaggccgg ccgccgcgta
    10621 cacctcgcgc tcgtcgagcg tctcgcgcgc gagcaactgc tccacgattg cgtcgagctt
    10681 gtcgcggttc tcccggagca gccgccgcgc ctcgtcgtag cactcgtcga tgaagcgccg
    10741 cacctcctcg tccaccgcgt cgagcagggc gtcggaggtg cccatcatcc gcgggtcgcc
    10801 gtccggcggg tacaccgaga tcttgccgac ccgctccgac atcccccacc gccccaccat
    10861 ccccctcgcc acccgcgtga ccatctccag gtcgctctcg gcgccggtgg tgacgacgcc
    10921 gaagacctcc tgctcggcgg ccatgccccc gagcgcaccg atgatgcggc cgcgcaggta
    10981 gttcgcgtcg tagccgtagc ggtcggcctc gggggtcgag agcgtcacgc cgagcgcccg
    11041 gccgcgcggg atgatcgaca ccttgcggac cgggtcggcg cccggctgca gcatgccgat
    11101 cagcgcgtgg ccggcctcgt ggtacgccgt gcgccgccgt tcgggctcgg agatgacgac
    11161 cttgcgcgcg gtgccgagct ggatcttctc gagggcgtcg aacaggtcgc gctgggtgac
    11221 ggtgtcgcga tcctgccgcg cggcctgcag cgccgcttcg ttcaccaggt tcgccagctc
    11281 ggccccggtc atgccgggcg tggatgaagc cacggcctcg aggctcacat cgggcgccag
    11341 cggcaccttt cgcgtgtgca ccttcagaat ctcggcgcgc cccttctggt cgggggcgtt
    11401 caccgtgatc gtccggtcga accggccggg ccgcagcagc gcgggatcga gcacgtcggc
    11461 cctgttggtg gcggccagga cgacgacccc ctcggtgccc gagaacccgt ccatctcggt
    11521 gaggatctgg ttcagtgtct gctcgcgctc gtcgtggccg ccgagggacc gggccccggc
    11581 gcgcgtgcgc ccgatcgtgt cgatctcgtc gatgaagatg atggcggggg cgaccttcct
    11641 ggcctcgttg aacagctcgc ggacgcggct cgcgccgacg ccgacgatca tctcgatgaa
    11701 ctccgacgcg ctggcgctga agaacgggac cttggcctcg cccgcggtcg cgcgcgccag
    11761 cagcgtcttg ccggttcccg gcgccccggt gagcaggacg cccttgggcg cccgggcgcc
    11821 gacgcgccgg tacttgcccg ggtctcggag gaagtccacg atctcgctga tctccgcctc
    11881 gacctcgtcg atcccggcaa cgtcgtcgaa agtcacccgc accgtctcgg gatccaccgg
    11941 cttgcgcggg cggcctccga gcagcccgcc gcccatcgac gcccgctgcc gcctgaacat
    12001 ccagacccag aagccgacca gcagcaggat cggcagcatc gagatcagca ggttggccag
    12061 caggccgcgg tgctcgacca gcggggtcgc ccgcacgctc gccccgcctg ccgccagctc
    12121 ggcgagcagg tcgtcggcgg cgaaagtcgg gcgttcggtc gtgaactgct ggtaggtgtg
    12181 cccctgctcg cccggcagct cggccggctc cttcagctgc ccttcgatcg tgtcgccgcg
    12241 ggcgaacacc tccttcacgt tcttgcgcga gacctgcgcc ttgaactcgg tgtaggagat
    12301 ggcctgcggg ccgctcatcc ggtcctgcac ggtgagcagg ccgaacagta ccaggtagcc
    12361 gacgaagaac gcggccatcc acgtccagcg gcgccgggac ggcggcggct gttcgccctt
    12421 cggcagtcct tcggtgcgcc agggcttggt ggccggctcg tcgggcttgc ggcccggctg
    12481 ggggctggtg gcctgtttct ggggctcagg cttcggctgc ggctccgtca cgggaatcct
    12541 cccggcggga agggcacaaa cagacggtac acggggattg tacaggacgc ggcaggcggt
    12601 cagccgagca gcagctcccg gacggcctgg atctcctcgt cgttgacgac gtgtcccatg
    12661 ccgggataga tgcgctcgtc caccagcgcc ccgagcgttc ggaagacgcc ggcggtttcg
    12721 tgcacgcgct ccaccgggat gtgcgggtcg acgttgctgc acccgaggaa gacgggcgtt
    12781 gcctccatcg agccctcgta cgctcgcggc gtgccggggg gcccgatcac gcccccgctc
    12841 aggccgacga ccgcggcgta ccggcgcgcg tgccgcgccg cgtactcgag ggcgaggcag
    12901 gcgccctggg agaacccgag cagcgccacg cgcgcgggcg ggacaccggc ggccccgagg
    12961 cgcgcgaccg cacgatccac cagcgcgagc gccgagtcga ggtgcggctg gttgcggtcg
    13021 agcggctgga ggaacgagta gggataccag gtgtgccccg cggcgtcggg ggcgagatac
    13081 agcacgtcgg caaggtccag ctcgtcggcg aggctgagga tgtcggaggc ggtgctgccg
    13141 cgcccgtgca gcaggatcgc gcagaggcgc gcggcggccg ggtcggggcc gcgccgaagg
    13201 acgggttgcg agccgtgggg gtcggcggcg ttcatggtgt cggcttcagc gctggcgagc
    13261 ggctctccac ttccggatag cctcggcgtg cctgggatag tggccgtagg tgtcggcacg
    13321 gagccgcctg acgaaccgcg actcggcggc cagcgcctcg tcgggcgcct gctcgacgaa
    13381 cctgaccagc cggcgatgcg tgccatcgag ccggcgcagc acctcggcca gcgggagggc
    13441 ggccttctcg gccgtcgtct tcgcgttgaa ggcgtcgatg ccgccgtaga gcgtggagta
    13501 gcgggggggc cgcttccctt cgaggatcac tggcaggtac ttcagcgcct cctcttccca
    13561 ggtcgtgacg tgggcgatga tgtccctgac cgaccaggcg cgcgtgacgc cgggcgcggt
    13621 aagctcctcg tcgctcagcc cggcgaacga cccggtgaag tcgcgccact tctcgtcgag
    13681 gcgcgcaagc agccgcgtgc ggtccatgaa ggcattgtaa ggtgccttgc ggcagcgcgc
    13741 ggtactggaa gttgcgaacg cgctcctcat gacggcacct ccaacgctct ctgctagcgc
    13801 tgcacccggc cgctggcgtc gccgccggcc aggcgttcga cttccgctac cgaaacacga
    13861 ttgaaatcgc ccggaatggt ctgcttgatc gcgctcgccg ccaccgcgaa gcgcagcgcc
    13921 gacgccgcgt cccggccggc ggcgaggctg tagacgaggc ccgccgcgaa actgtccccg
    13981 ccgccgatcc gatcgaccag gcgcacgacg tagcgctggg cctggtggaa cgccccggtc
    14041 gcggcgtcga acagcaccgc gctccaggcg ttgtcgcttg ccgaaaggct ctccctgagg
    14101 gtgaccgcca cctgcttgac gccgaactcg cgcaccacct gcccggccgc ggcgcggtag
    14161 gcctcggcgt cgaggacgcc cgccttcacg tcggcgcggg ccacctcgat gccgagcgac
    14221 gcctggaggt cctcctcgtt ggcgatggcc acgtccacgt acggcatcag cgcgcgcatg
    14281 gtccgctgcg cgtcggcctt cgaccagagc ttggcgcggt agttgagatc gaggctgacg
    14341 cgcacgcccg ccttcgcggc ggccgccagc gcctcggccg tgcacgccgc cgggccggga
    14401 ccgagggccg gcgtgatgcc ggtgacgtgg aaccaggccg cgccgtcgag taccgcctcc
    14461 cacgggattg ccccgggctg cagctcgccg atcgccgagt gcgcgcggtc gtataccacc
    14521 agcgagggac gctggctggc gccggtctcg gcgaagtaga tgccgagccg gctcccgccc
    14581 cgcagcaccg gctcgacccg aaccccctcg gcgcgcagcg ccctgagcgc cgcgtcgccc
    14641 accgggttgg cgggcaggcg ggtgacgtag tggctgtcga ggccgaagtg cgcgaggctg
    14701 acggcgacgt tcgcctcgcc gccgccgaag gtggcgtgca ggagcggcga ctggaacagc
    14761 cgctcgaacc cgggcgggct gagccgcagc atgatctcgc cgaagcagac ggtcttgatc
    14821 actgctacct cccctgcccg cgcgcgcgcc gcacggcctc gatgaagtgc gccgcgttgg
    14881 ccgcgatccg gtcgaactgc ccggccttga tcgcagccct gtccaccagg gcggtgccca
    14941 cgccgatggc cgccgcgccg gccgcgatcc actcccccgc attgtcgcgc gtcaccccgc
    15001 cggtcggcat cagccggacg tgcggcagcg ggccccgcat gtccttgaag aaggcaggac
    15061 ccaggacggt cgccgggaag actttcacga ggtcggcgcc cgcgtcccag gcgtcgagga
    15121 tctcggtcgg cgtgaagcag cccggcatca cggccacatc gtgcaggcgc gcgacctcga
    15181 gcacggcggg ccggaacacc gggctcacga cgaag
//
LOCUS       DAOPQX010000049        15000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4710_length_15000_cov_4.293744, whole genome shotgun sequence.
ACCESSION   DAOPQX010000049 DAOPQX010000000
VERSION     DAOPQX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 15000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15000
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4710_length_15000_cov_4.293744"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKK95_04395"
     CDS             <1..525
                     /locus_tag="PKK95_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17483.1"
                     /translation="LGLGFLVADLRAIANHLLYWKRRPGALLLWPAAYPPFFSLQLII
                     GGTLGLLISYNLVFRVTPAEELFGEGMMFIYYAYAVPLAHRVERGFYGDGVWTDAGFL
                     PYHRVGSITWREEPEPVLLLADRERAQARRLVVPGELYGAVRRLLRDLIDSRTIHFSR
                     PGIDLGIREEKDDA"
     gene            542..886
                     /locus_tag="PKK95_04400"
     CDS             542..886
                     /locus_tag="PKK95_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17484.1"
                     /translation="MLGVQVVPKVLEVLVPKVLEVLVPKVPEVLVPKVPEVLVPKVRE
                     GLVPKVREGLPRRSRAPRGEGGCWCWRCAGGSYSASPAPARNRSGWRGSQTPFGRAGQ
                     PRKRESSGSPAG"
     gene            1159..2022
                     /locus_tag="PKK95_04405"
     CDS             1159..2022
                     /locus_tag="PKK95_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOC17485.1"
                     /translation="MAPRSTWKGFLKVSLVSIPIKVYPAAESSASLSFNQLHGECRTR
                     IQQKRWCPHCNREVANSELVKGYEFEKGRYVVLEDEDFAKVRTESTRVINLVQFSGEE
                     SLDPMYYDRAYYLAPDGPMAAGAYAVMREGMRGKIGVGKVALLGREYLVAVRPHQEGL
                     LMFTLHHAGEMRTPSQIEELRTLPDVKADEVALAKRVIGTFEGELDLSDFKDEYKEGL
                     REVIQARIAGEEVATPPAVEAPTNVVNLMDALRKSLDAVSSSKKKPAKAELPSRKAPA
                     RAEGGKRRKAG"
     gene            complement(2030..2803)
                     /locus_tag="PKK95_04410"
     CDS             complement(2030..2803)
                     /locus_tag="PKK95_04410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOC17486.1"
                     /translation="MTFTGIIGKACSFPLRAIIAACVALGISPNVLTFVGMMVNITAA
                     WLLGLGLFRTAGFVMLAANIFDYIDGKVARERNRETAFGAFWDSVMDRFSDITLFIGL
                     IYLYTKLGRADYVVITALAMMFSVMTSYTRARAESIIEKCKVGFMERPERIVLFMIGA
                     FTNRMAAVLWVILVLSIVTVADRIYFTWRVTHRRPERKLPLPALVLWRAFFWTDERAS
                     WQYDLWVIAILAFVWLTPPAWIGDPVAEGPGLLGWFLPL"
     gene            2904..4733
                     /gene="uvrC"
                     /locus_tag="PKK95_04415"
     CDS             2904..4733
                     /gene="uvrC"
                     /locus_tag="PKK95_04415"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOC17487.1"
                     /translation="MPIQELKSRIDRLPEQPGVYLFANARGETIYVGKARSLRDRVRS
                     YLGARGTNAKTDALLDEASAVEVIVTDSVVEALALENNLIKQRMPRFNILLRDDKNYP
                     FLQLTVTEEYPRVRVVRGVERDGNVYAGPFMPASLARRTMGLTHRLFGIRSCNEELNG
                     RRPRPCLEYDIKRCLAPCVASICPPEQYRRAVEDAQLFLEGRTDELAAHLRERMTRAA
                     MEERFEQAAQLRDALHTVETLRDRQQKMAGVELGDRDVWGVKLGTAGAAIQVFEVRRG
                     RVVERVTLITEADSAGTLAGADEALAELLEAAIPQFYEEREIPPEIHVPAGLGEAEAA
                     EAWLSGRAGRRVRLVVPQRGGKRALLELAARNASLAYESRFNHERAANYDGLDTLRVA
                     LNLPCFPRRIECFDISTIQGSETVASMVVCEDGRMLRSAYRKYRIRGEGSRFLDDFAA
                     MAQVVRRRYRSVLESGTDAPDLIVIDGGKGQLGAAYASLEELGLSNLVAVGLAKKEEL
                     VYTRDRDEPLALPAGSAALRLLQMIRDEAHRFAVAFHRQSRRARDLTSELDAIPGIGP
                     RRRRALLSAFGSLAGVRRASREQLTPVVGARAADAVLAHFAGR"
     gene            4861..5526
                     /locus_tag="PKK95_04420"
     CDS             4861..5526
                     /locus_tag="PKK95_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOC17488.1"
                     /translation="MDFDFAAIFIAFVALLLSLTVHEAAHAWAADRLGDPTARALGRL
                     SLNPAVHVDPLGTLVFPLVAMVTGAPLIGWAKPVPIAPWRFDRPRQASMLVAFAGPLS
                     NLALAFVAAIALRMLPLEALSSLGPAGQAAAADSRILAPVARLAWGVLSINLLLAIFN
                     LLPIPPLDGSSVLAGLLPERAAAGLDRLRPYGFLLLYALLLSGGFSLIVSPPYLLLLS
                     WLT"
     gene            5523..6551
                     /gene="trpS"
                     /locus_tag="PKK95_04425"
     CDS             5523..6551
                     /gene="trpS"
                     /locus_tag="PKK95_04425"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOC17489.1"
                     /translation="MKPRVVSGMRPTGKLHLGHLVGALRNWERLQDQYDCFYFVADWH
                     ALTSEYADPSAITANALDNVADWLAAGLDPARSTLFVQSLVPEHAELHLLLSMTTPIP
                     WLERVPTYKEQREQLSDKDLSTIGFLGYPLLQTADVAIYDAEFVPVGEDQVPHLELSR
                     EVVRRYNNFYGERLGVKDGEPIIKPVLREPQPLLTTVPRLPGLDNRKMSKSYGNAIDL
                     SDTAEDVRKKVMSMYTDPKRVRADIPGTVEGNPVFIYHDAFNSNLEEVEDLKARYRLG
                     TVGDVEVKKKLIAAVNGVLEPMRQRRAEVLARPGYVREVLFEGSRRARALAQETMARV
                     REAMKITY"
     gene            6595..7347
                     /locus_tag="PKK95_04430"
     CDS             6595..7347
                     /locus_tag="PKK95_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOC17490.1"
                     /translation="MDTQAEIFESSTDSYAVKLDNFEGPLDLLIHLIKRNELNVYDIP
                     IALITGQYLEYLELMRELNLDVAGEFLVMAATLIHIKSRMLLPRPDPAQEDPEEDPRE
                     ALVRRLLEHQRYKQAAELLHERETLREAQWQRPDGRVAPIAGEEYEPELEVDLFSLIA
                     AFKSVLERAKHRPKVVLPIEQVSIEARIEQLLARLTGSKACGFEDLFDDVSAKGDLIV
                     TFLALLEMIRLKLVRVFQAGPFGAIRIYRQVA"
     gene            7536..8189
                     /gene="scpB"
                     /locus_tag="PKK95_04435"
     CDS             7536..8189
                     /gene="scpB"
                     /locus_tag="PKK95_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOC17491.1"
                     /translation="MGQPSAELKAIIEALIYASPDPLTPKTLFKVLESEPREDVQASL
                     DELLRDYADGRGGLQLVEVAGGYQIVTRPELSEWVRRLFHERKTQKLSLAALETLAVI
                     AYKQPITAPELTDIRGVNASGVIATLLERRLIKIAGRKQVVGRPFLYATTREFLIRFG
                     LRDLDDLPKIEDMAEVLGFEAPIGLAEAGPSETLLPLDGEADPDRTLDVPPPEEPVH"
     gene            8212..8976
                     /locus_tag="PKK95_04440"
     CDS             8212..8976
                     /locus_tag="PKK95_04440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOC17492.1"
                     /translation="MAHGRLQKILSSAGVASRRAAERLMAEGRVSVNGEVVRELGTKA
                     DPEADDIRVDGRPIGRASRRLYLLLNKPRGYVTTRSDPEGRPTVLDLLPGVREYVFPV
                     GRLDYDSEGLLLLTNDGELAARLTHPSHEVPREYHARVRGVPDPHALDRLAGGVTLDG
                     RRTAPARAVLLERGLGQAGDQALVSLTLHEGRTRQVRRMLEAVGHPVVRLRRVRIGPI
                     ADPLIPLGRYRHLLPEEVDALRRAAASTARPRRGEG"
     gene            8987..11839
                     /locus_tag="PKK95_04445"
     CDS             8987..11839
                     /locus_tag="PKK95_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17493.1"
                     /translation="MPRSALAIAAIVLSVVSTGKGFAAQEAESLAVPGGVASFAGALK
                     VSEDLPRARLMLAAIRILWEAPEGSDPNANRRRAALLSYLQAVDQEAPSRTAGDGGEV
                     PGLLPAAAWSSVLREIGATGGSTFTAILRSRRAALLYHGLASLDAATRDGLARRPRLL
                     AAISRDNRVALFATFGRSFRLRTGHIDPPGGAEAAPLWEALAGERLDEPDAFITRALD
                     LHGGRFALLYDAVAHLDAPARRFALGLRFGPSRRLERFKAFYDASAAALRSWEPQRRP
                     FERVPYDAVHLLLSTRFESDGQLSGPAWPPLWRAVFDPVIDPGTALASLGNEDLCDAA
                     KMVEMVSVADPVERQRRAQAWLFGQRVFRRPPRQSARDLLPVLKAIVRYPSLFAVLDR
                     MGIEALPTYTAALQAADRLGAADRRLWLFQAALAILERSRMARAIDAAAADRLVQALS
                     AAASADGERYGAAVLRWIDGGLLAAAGRPLLSPHLDASDRPVETRLLAAMAGSVDSPA
                     NRQLLSLPPVEWEGLAYRLDPGASMLRRAVEVRARQGGRSLDAVLALSRLADSLAAAA
                     STEEAVEVASRLPAVVEGLQEQAGPGRRRLAVAGGEPAIGRLVARVTEGARQLKPRDA
                     KAAEALARPLAEAVDSLLAEVLGAIVYAAHLGEPDGPALLAGDPSAVHDFRFFPPAME
                     LGRRPAWHLPVEVRDREMAWGVSGSLVNLDSALGRLSLKRPFSETLPLPPALSDQDRR
                     TLIENAVLSVPQDFTEAGRERLLAAVERGRARVEALGPATDEIERLAADVDLDEWRAE
                     ALAWTIANEPSRRLDFLSLGELARAGGLDPRGDASLDAWGTSEYARDGALVNRYPPRL
                     DLTIMAGRRGVRATASLVPDLAIGLAESSKRLGLPAALTPGLLLVATQRFIETVQVVH
                     ADDWLTMVTHARRVAREGVDDYVALLTVSGPLVTED"
     gene            11842..>12861
                     /locus_tag="PKK95_04450"
     CDS             11842..>12861
                     /locus_tag="PKK95_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC17494.1"
                     /translation="MGSSPHPLAAALCAAVLIAVAAPLAQAPAPAVVFVFPADGDYVS
                     GPVTLKAELRPHDQAIKSLSLFADGKLVCALERPPFECDWDAGRRVSEHALRAVAVLP
                     DGRRLARGVRTRGVPYAEAVDVDVVHITASVTDGDGRFVKGLARQRFKVFEDNAAQNV
                     TYFAAENVPLEIIVAVDVSGSMADAMPQLKAATKKFLSALRPADRVTLIGFNDNVFTL
                     ARPGVDLAARLRAVDRLAPWGGTALYDVIVQAVDQLGRHAGRRVLVVFTDGEDLNSHV
                     AVEVAEGRLEASDVVLYPIGQGRAVEGAKSLKAILERLAQKTGGRAFFEEADRLEAVF
                     GNILEE"
     assembly_gap    12862..12961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12962..>15000
                     /locus_tag="PKK95_04455"
     CDS             <12962..>15000
                     /locus_tag="PKK95_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17495.1"
                     /translation="RPVPGLQPADFRVLVDGRPRQVLTAEFLSSAASPAAAADAERGA
                     DRGELDAVFESVYVGNHRAGGQRATPSRTVMIAIDQSGFSLGAGRNAATTARGLLDLL
                     HPNDRVGLAVFPQPGVFVRPTRDHQRVRLALGQVTGAPDPFPRSEISLNVADALAWTS
                     GDTMSRDLVVQKYCSHTAAMTCQPELDRAASQIISYAQRQSLTTLQGLGDVINAATAD
                     GDPVAVVLVSSGVYASGRATQLGVDGEARTLARIAASSRAMLYAMYVETGFLDRDSME
                     RARTAGFSSEDGDMRLEGLRHLAGLSGGTVTRVVSGSDEGFRRVSLELSAYYLLGVEG
                     MPGDRDGNRHRLEVSVARPGLTVRSRDELYLAPPRKTTAEQSVVEALDAPGLVGDLPI
                     RLSTQMMREPGTDKVRMVISAAVGREVGRPAPVRVGYVLRGAGSKGTAAGSGVQAREL
                     PVVGDGPDASWSFIETAQLTPGRYLLRLAAIDEEGRLGSVQQVVDAMLVGGDGGTFSD
                     LLLVDPARTVKGTFTPAADGRMTADSLEAFLEIYPGRGASVRSVAFEVDDAPGGAPLI
                     TRSVVPERREDGRLVAGINFELRSLPPGVYALDARILDGERVLGRVSRPFLLERRSVG
                     SAGAPRAALAFAATGGLVRAFSGREALRPDALGYFLNRMSASEVAATGTEVAEAA"
BASE COUNT         2169 a         5199 c         5360 g         2172 t
ORIGIN      
        1 ctcgggctcg ggttcctggt ggccgacctg cgggccatcg ccaatcacct gctgtactgg
       61 aagaggcgcc cgggcgcgct cctgctctgg cccgcggcct acccgccctt cttctcgctg
      121 cagctcatca tcggcggcac gctcggcctg ctgatttcct acaacctggt gttccgtgtc
      181 acccctgccg aggagctgtt cggcgagggg atgatgttca tctactacgc ctacgcggtc
      241 ccgctcgcgc atcgggtcga gcgcgggttc tacggcgatg gcgtctggac ggacgccggg
      301 ttcctgccct accaccgcgt cgggtctatc acctggcgcg aggagcccga gccggtgctg
      361 ctgctggcgg atcgcgagcg ggcgcaggcg cgccgccttg tggtgccggg cgagctgtac
      421 ggcgccgtgc ggcgcctcct gcgcgacctg atcgacagcc gcaccatcca cttctcccgc
      481 cccggcatcg acctcgggat ccgcgaagag aaggacgacg cgtagggaag cgaacagcgt
      541 cgtgctgggg gtgcaggtgg tgccgaaggt gctggaggtg ctggtgccga aggtgctgga
      601 ggtgctggtg ccgaaggtgc cggaggtgct ggtgccgaag gtgccggagg tgctggtgcc
      661 gaaggtgcgg gaggggctgg tgccgaaggt gcgggagggc ctgccgcgcc gtagccgcgc
      721 accgcgcggc gaaggcgggt gctggtgctg gaggtgcgcg ggaggctcct acagcgcctc
      781 gccagctccc gcccgcaacc ggagcgggtg gcggggcagc cagacacctt ttggccgagc
      841 cgggcagcct cgcaagcggg agtcatcagg gagtccggcc ggctgatgcc ggccggcgac
      901 cttcgcccgc gcgtcgaggt gcccggccgg acaaaacggt gtcgctgccc cgacaggacc
      961 cggatcccga tcatggttgc gggccggaga cgccctctgg gcgaccaccc gccttcgcct
     1021 ccacgaggct tcggcgggtc gcccctacgg tgccgctcgg gagccgacgc aaccgccccg
     1081 cgctcgtttc ttgctatatt ggcagatggg tcttcccgaa caccggcgtc cggcaacctt
     1141 cgcgcccgag gtgtgtgcat ggctccccga tccacctgga aaggcttcct gaaggtcagc
     1201 ctcgtcagca tcccgatcaa ggtctacccg gctgccgagt cgtcagcgag cctctcgttc
     1261 aaccagctcc acggtgagtg ccgcacccgc atccagcaga agcggtggtg cccgcactgc
     1321 aaccgcgagg tggccaacag cgagctggtc aagggctacg agttcgagaa ggggcggtac
     1381 gtcgtcctcg aggacgagga cttcgccaag gtgcggaccg agtcgacccg cgtcatcaac
     1441 ctggtgcagt tctccggcga ggagtccctc gacccgatgt actacgaccg ggcctactac
     1501 ctggcccccg acggcccaat ggcggcgggc gcctacgcgg tgatgcgcga gggcatgcgc
     1561 gggaagatcg gcgtcgggaa ggtggcgctg ctcgggcgcg agtacctggt cgcggtgcgg
     1621 ccgcaccagg aggggctgct gatgttcacc ctgcatcacg ccggggagat gcggacgccg
     1681 tcccagatcg aggagctgcg gacgctcccc gacgtgaagg ccgacgaggt ggccctggcc
     1741 aagcgggtga tcggcacctt cgagggcgaa ctcgacctct ccgatttcaa ggacgagtac
     1801 aaggaggggc tgcgcgaggt catccaggcg cggattgccg gcgaggaagt ggccaccccg
     1861 cccgcggtcg aggccccaac caacgtcgtc aacctcatgg acgcgctgcg gaagagcctc
     1921 gacgccgtca gctcctcgaa gaagaagccg gccaaggccg agctgccgtc ccgcaaagca
     1981 cctgcacgcg cggagggagg gaagaggagg aaggcggggt agacgccacc tacagtggca
     2041 ggaaccatcc cagcagcccc gggccctcgg cgacggggtc gccaatccag gcgggcggcg
     2101 tcagccacac gaacgcgaga atcgcgatca cccagaggtc gtactgccag ctggcccgct
     2161 cgtcggtcca gaagaacgcc ctccagagaa ccagcgcggg cagggggagc ttgcgctcgg
     2221 gcctgcggtg cgtcacccgc caggtgaagt agatccggtc cgcgacggtg acgatcgaca
     2281 ggacgaggat cacccagagc accgcggcca tgcggttggt gaaggcgcca atcatgaaga
     2341 gcacgatccg ctcgggccgc tccatgaacc ccaccttgca cttttcgatg atcgactcgg
     2401 cccgggcgcg cgtgtagctc gtcatcaccg agaacatcat cgcgagcgcg gtgatgacga
     2461 cgtagtcggc gcggccgagc ttcgtgtaga ggtagatcag gccgatgaag agggtgatgt
     2521 ccgagaagcg gtccatcacc gagtcccaga aggccccgaa ggcggtctcg cggttgcgct
     2581 cgcgggccac cttgccgtcg atgtagtcga agatgttggc cgccagcatc acgaagccgg
     2641 ccgtgcggaa gagcccgagc cccagcagcc aggccgccgt gatgttcacc atcatcccga
     2701 cgaaggtcag gacgttcggc gagatcccga gcgcgacgca cgcggcgatg atggcgcgca
     2761 ggggaaacga gcaggccttg ccgatgatgc ccgtgaacgt catgagcggc ttccggctgc
     2821 gtggacggac cccgcacggg ccccaaaggc ggcgcctata attgtgttgg gaaccgcgcc
     2881 gggaaaacgc gccatcttag tccatgccca tccaggaact caagagcagg atcgaccggc
     2941 tgcccgagca gccgggggtc tacctgttcg ccaacgcccg gggtgagacg atctacgtcg
     3001 gcaaggcgcg ctcgctgcgc gaccgggtgc gcagctacct gggggcgagg ggaaccaacg
     3061 ccaagaccga cgccctgctc gacgaggcca gcgcggtcga ggtcatcgtc accgactcgg
     3121 tggtcgaggc gctggcgctc gagaacaacc tcatcaagca gcggatgccc cgcttcaaca
     3181 tcctgctgcg tgacgacaag aactacccct tcctccagct gaccgtcacc gaggagtacc
     3241 cccgggtgcg ggtcgtgcgc ggcgtcgagc gcgatgggaa cgtttacgcg ggccccttca
     3301 tgcccgcgag cctggcgcgg cggacgatgg ggctgacgca ccgccttttt ggcatccggt
     3361 cgtgcaacga ggagctgaac ggccggcgcc cccgcccgtg cctcgagtac gacatcaagc
     3421 ggtgcctggc cccctgcgtg gcctccatct gcccgcccga gcagtaccgg cgggccgtgg
     3481 aagacgccca gctgttcctc gaagggcgga ccgacgagct ggccgcgcac ctccgcgagc
     3541 ggatgacccg tgccgcgatg gaggagcgct tcgagcaggc ggcgcagctg cgcgacgcgc
     3601 tgcacaccgt cgagacgctg cgcgaccggc agcagaagat ggccggcgtc gagctgggcg
     3661 accgcgacgt ctggggagtg aagctcggga cggccggcgc cgccatccag gtgttcgagg
     3721 tcaggcgggg ccgcgtggtc gagcgcgtga cgctcattac cgaggctgac tcggcaggca
     3781 cgctggccgg agcagacgag gcgcttgccg agctgctcga ggcggccatc ccgcagttct
     3841 acgaggagcg ggagattccc cccgagatac acgtgccggc cgggctcgga gaggccgagg
     3901 cggccgaggc gtggctgagc ggccgggccg ggcgccgcgt gcgcctggtg gtgccgcagc
     3961 ggggaggcaa gcgggccctg ctcgagctgg cggcgcgcaa cgcgtcgctc gcctacgagt
     4021 cgcgcttcaa ccacgagcgg gcggcgaact acgacggcct cgacacgctg cgcgtggccc
     4081 tcaacctgcc gtgctttccg aggcggatcg agtgcttcga catctccacc atccagggca
     4141 gcgagacggt ggcctcgatg gtggtgtgcg aggacggccg gatgctgcgc tcggcctacc
     4201 gcaagtaccg catccgcggc gagggctccc ggttcctcga cgacttcgcc gcgatggcgc
     4261 aggtggtgcg gcgccgctac cgctcggtgc tcgagagcgg gaccgacgct cccgatctca
     4321 tcgtgatcga cggcggcaag ggacagctgg gcgcggcgta cgcctcgctc gaagagctgg
     4381 ggctctcgaa cctggtggcc gttggactgg cgaagaagga ggaactggtc tacacgcgcg
     4441 accgggacga gccgctggca ctgccggccg gcagtgccgc gctccgcctg ctccagatga
     4501 tccgcgacga ggcccaccgc tttgccgtcg cgttccaccg ccagtcgcgg cgggcgcgcg
     4561 acctcacctc ggagctcgac gcgatccccg gcatcggccc gcgccgccgg cgggcgctgc
     4621 tctcggcgtt cggcagcctg gccggcgtcc ggcgcgccag ccgcgaacag ctcacgccgg
     4681 tggtgggagc ccgcgccgcc gacgcggtgc tggcgcactt cgccggccgc tgacaccagc
     4741 gccgcccggg tccgaatgta gaatgcggca cgttgaacgg gtggaaggcc ggcctgccgt
     4801 ggtagcctga taagtccggc aagtggccgg gcctccaggg ccggacgccg cgtacccatc
     4861 gtggacttcg atttcgccgc catcttcatc gcattcgtgg cgctgctgct gtcgttgacg
     4921 gtccacgagg ccgcccacgc gtgggccgcc gaccgcctgg gcgatccgac ggcgcgcgcg
     4981 ctcgggcggc tctcgctgaa cccggccgtg cacgtggacc ccctcgggac gctggtgttc
     5041 cccctggttg cgatggtgac cggcgcgccg ctgatcggct gggccaagcc ggtgccgatc
     5101 gccccgtggc gcttcgatcg gccgcgccag gccagcatgc tcgtggcatt tgccggcccc
     5161 ctcagcaacc tcgcgctggc gttcgtggcc gccatcgccc tgcggatgct gccgctcgag
     5221 gccctttcct cgctcgggcc ggccggccag gcggccgcgg ccgacagccg gatcctggct
     5281 cccgtggcgc gcctggcgtg gggcgtgctg tcgatcaacc tgctcctggc gatcttcaac
     5341 ctgctgccga tcccgccgct cgacggcagc agcgtgctgg ccggcctgct gcccgagcgg
     5401 gcggccgccg gcctcgatcg ccttcgaccg tacggcttcc tgctgctcta cgcgctgctg
     5461 ctctccgggg gcttcagcct gatcgtcagc ccgccgtacc tcctcttgct gtcgtggctc
     5521 acatgaaacc gcgcgtcgtg tcgggcatgc gcccgacggg gaaacttcac ctggggcacc
     5581 tggtcggggc gctgcgcaac tgggagcgcc tgcaggacca gtacgactgc ttctacttcg
     5641 tcgccgactg gcacgcgctg accagcgagt acgcggaccc ctcggcgatc acggcgaacg
     5701 ccctcgacaa cgtcgccgac tggctggccg ccgggctcga cccggcgcgc tcgacgctgt
     5761 tcgtccagtc gctggtgccc gagcacgccg agctgcacct gctgctgtcg atgacgacgc
     5821 cgatcccgtg gctcgagcgg gtgccgacct acaaggagca gcgcgagcag ctgagcgaca
     5881 aggatctctc cacgatcggc ttcctcggct acccgctgct gcagacggcc gacgtcgcga
     5941 tctacgacgc cgagttcgtg ccggtggggg aggaccaggt gccgcacctg gagctgtcgc
     6001 gcgaagtcgt gcgacggtac aacaacttct acggcgagcg gctcggcgtg aaggatggcg
     6061 agccgatcat caagcccgtc ctccgggagc cgcagccgct gctgaccacg gtgccgcgcc
     6121 tgccggggct cgacaaccgg aagatgagca agagctacgg caacgccatc gacctctccg
     6181 acaccgccga ggacgtgcgg aagaaagtga tgtcgatgta caccgacccg aagcgggtgc
     6241 gggccgacat cccgggcacc gtcgagggca acccggtgtt catctaccac gatgccttca
     6301 actcgaacct cgaggaggtg gaggacctga aggcccgcta ccgcctgggc acggtgggcg
     6361 acgtggaggt gaagaagaag ctgatcgcgg cggtgaacgg cgtgctcgag ccgatgcgcc
     6421 agcgccgcgc cgaggtgctc gcccgccccg gctacgtgcg cgaggtgctg ttcgagggct
     6481 cgcgccgcgc ccgggcactc gcgcaggaga cgatggcgcg cgtgcgggaa gcgatgaaga
     6541 tcacgtactg aacacgctct ttgtgacctt tgtggttcat ttctccgggg tttgatggac
     6601 acgcaggccg agatcttcga gtcgtcgacc gacagctacg cggtgaagct cgacaacttc
     6661 gagggaccgc tcgatctgct catccacctg atcaagcgga acgagctgaa cgtctacgac
     6721 atcccgatcg cgctgatcac cgggcagtac ctcgagtacc tcgagctgat gcgcgagctg
     6781 aacctcgacg tcgccggcga gttcctggtg atggccgcga cgctcatcca catcaagtcg
     6841 cggatgctgc tgccgcggcc cgaccccgcg caggaagacc ccgaggagga cccgcgcgag
     6901 gcgctcgtgc ggcgcctgct cgagcaccag cgctacaagc aggcggccga gctgctccac
     6961 gagcgcgaga cgctgcggga ggcacagtgg cagcgccccg acggccgcgt cgcgccgatt
     7021 gccggcgagg agtacgagcc cgagctggag gtcgatctct tcagcctcat tgccgccttc
     7081 aagtcggtgc tcgagcgcgc caagcaccgg ccgaaggtcg tgctcccaat cgagcaggtc
     7141 tccatcgaag cgcgcatcga gcagttgctg gcgcgcctca ccggcagcaa ggcctgcggc
     7201 ttcgaggacc tcttcgacga cgtgagcgcc aagggcgacc tgatcgtgac cttcctcgcg
     7261 ctgctggaga tgatccggct gaagctggtg cgggtgttcc aggccgggcc ctttggcgcg
     7321 atccggattt acaggcaggt ggcgtagtgg acagcgaaga gaaagaagga caggcgcccc
     7381 cgcctgaagt ggcacaggcg tccccgcctg tggtggatga agctgctgaa gtagcacagg
     7441 cgtcctcgcc tgtggcggat gaagccggtg aagtagcaca ggcgtcctcg cctgtggcgg
     7501 atgaagccgg tgaagtggaa caagcctctc cgcctgtggg ccagccctcc gccgagctga
     7561 aggccatcat cgaggccctg atctacgcct cgcccgaccc gctcacgccg aagacgctct
     7621 tcaaggtgct cgagtccgag ccgcgcgagg acgtgcaggc ctcgctcgac gaactgctgc
     7681 gcgactacgc cgacggccgg ggagggctgc agctggtgga ggtcgccggc ggctaccaga
     7741 tcgtcacgcg tcccgagctg tcggagtggg tgcgccggct gttccacgag cgcaagacgc
     7801 agaagctctc gctcgccgcg ctcgagacgc ttgccgtcat cgcctacaag cagccgatca
     7861 ccgcccccga gctgaccgac atccgcggcg tcaacgcctc cggcgtcatc gccacgctgc
     7921 tcgagcgccg gctgatcaag attgccgggc gcaagcaggt ggtcgggcgg ccgttcctct
     7981 acgccaccac gcgcgagttc ctgatccgct tcggcctgcg cgacctcgac gacctgccga
     8041 agatcgagga catggccgag gtcctgggat tcgaggcgcc gatcgggctc gccgaagccg
     8101 gcccgtcgga aacgctgctg ccgctcgacg gggaggccga tcccgaccgc acgctcgacg
     8161 tgccgccgcc ggaggagccg gttcactgaa gcggctcagg actcagggct catggctcac
     8221 ggccgccttc aaaagatcct ctcctccgcc ggcgtcgcgt cgcggcgggc ggccgagcgc
     8281 ctcatggccg agggccgcgt ctcggtcaac ggcgaggtcg tgcgcgagct gggaaccaag
     8341 gccgaccccg aggccgacga catccgcgtg gacgggcggc caatcggccg cgccagccgg
     8401 cgcctgtacc tgctcctcaa caagccccgc ggctacgtca ccacacgatc cgatcccgag
     8461 gggcggccga cggtgctcga cctgctcccc ggcgtccgcg agtacgtgtt ccccgtcgga
     8521 cgcctcgact acgattcgga ggggctgctg ctcctcacca atgacggcga gctggccgcg
     8581 cgcctgacgc acccgagcca cgaggtgccc cgcgagtacc acgcgcgcgt gcgcggcgtt
     8641 cccgatcccc acgccctcga ccgccttgcc ggcggtgtca cgctcgacgg gcgccgcacc
     8701 gcgccggccc gggccgtgct cctcgagcgc ggcctcggcc aggccggcga ccaggccctc
     8761 gtctcgctca cgctccacga ggggcgcacc cgccaggtcc gccgcatgct cgaggcggtg
     8821 gggcacccgg tcgtgcggct gcgccgcgtc cgcatcggcc cgattgccga tcccctcatt
     8881 ccgctcggtc gataccgcca tcttctcccg gaggaggtgg acgcgctgcg gcgcgccgcc
     8941 gcctccaccg cccggccgcg gcgcggcgag ggttagaatt ccttttgtgc cgagatcggc
     9001 cctcgcgatt gccgcaatcg tcctgtcggt ggtttccacc ggcaagggtt tcgccgcgca
     9061 ggaggcagag agcctggccg tgccgggcgg cgtcgcctcg ttcgcggggg cgctgaaggt
     9121 cagcgaagat ctgccgcggg cccgcctcat gctcgcggcc atccgaattc tctgggaggc
     9181 tcccgagggg tccgatccga acgccaaccg ccgtcgtgcg gcgctcctct cgtacctcca
     9241 ggccgtcgac caggaggcgc cgtcccggac ggcaggcgac ggcggcgagg tgcctggcct
     9301 gctgccggcc gccgcgtggt cgtccgtgct ccgcgagatc ggagccacgg gcggttcgac
     9361 cttcacggca atcctgcgga gccggcgcgc cgccctgctg taccacggcc ttgccagcct
     9421 cgacgccgcc acgcgcgacg gcctcgcgcg ccgccccagg ctgctcgcgg ccatctcgcg
     9481 cgacaaccgg gtcgccctct tcgccacctt cggccgcagc ttccgcctgc gcaccggcca
     9541 tatcgacccg ccgggagggg cggaggcggc tccgctctgg gaagcgctgg cgggcgagag
     9601 gctcgacgag cccgacgcgt tcatcacccg cgcgctcgac ctccacggcg gccgcttcgc
     9661 cctcctgtac gacgccgtgg cgcacctcga cgcgccggca cgccgcttcg cgctcggcct
     9721 ccgctttggc ccctcccgca ggctcgagcg gttcaaggcg ttctacgacg cgtcggccgc
     9781 cgcccttcgc agctgggaac cgcagcgccg gcccttcgaa cgagtgccct acgacgccgt
     9841 gcacctgctc ctgtccaccc gcttcgaatc cgacgggcag ctctcgggac ctgcctggcc
     9901 gccgctctgg cgggccgtct tcgacccggt aatcgatccc ggcaccgcgc tggcgagcct
     9961 cggcaacgag gacctgtgcg acgcggcgaa gatggtcgaa atggtgagcg tcgccgaccc
    10021 ggtggaacgg cagcgccgcg cgcaggcctg gctgttcggc caacgcgtct tccgccggcc
    10081 cccgcggcag tcggcccgcg acctcctgcc cgtgctgaag gcgattgttc gctacccgag
    10141 cctcttcgcg gtgctcgatc gcatgggcat cgaggcgctg ccgacctaca cggccgccct
    10201 gcaggccgca gaccgcctcg gcgccgcgga ccgccgtttg tggctcttcc aggcggcgct
    10261 cgccatcctc gagcgaagcc gcatggcgcg cgcgatcgac gcggcggccg ccgacaggct
    10321 cgtgcaggcg ctttcggccg cagcgtcggc ggacggcgag cggtacggcg cggcggttct
    10381 gcggtggatc gacggcggcc tgctggcagc cgcgggccgc ccgttgttgt cgccgcacct
    10441 ggatgcctcc gaccggccag tcgagacgcg cctgctggcg gccatggcgg gctcggtcga
    10501 ctcgccggcg aaccggcagc tgctgtcgct cccgcccgtt gaatgggagg gcctggcgta
    10561 ccggctcgac cccggcgcct cgatgctgcg gcgcgccgta gaagtgcggg cccggcaggg
    10621 agggcgctcg ctcgacgcgg tgctggcatt gtcgcgcctg gctgactcgc tggcggctgc
    10681 cgcgtcgaca gaagaggccg tcgaggtggc cagccggttg ccggctgtcg tggaaggctt
    10741 gcaggagcag gccggcccgg gccggcgccg cctcgccgtg gcaggcggcg agcccgcgat
    10801 cggcaggctg gtcgcgcgcg tgacggaagg cgccaggcag ctgaagccgc gggatgcaaa
    10861 ggcggcggaa gccctcgcgc ggccgttggc cgaggcggtg gactcgctgc tcgcggaagt
    10921 gctcggcgcc atcgtctacg ccgcgcacct gggcgaaccc gacgggccgg cactgctggc
    10981 cggcgatccg tccgcggtac acgatttccg cttcttcccg ccggccatgg agcttggccg
    11041 gcgtccggcc tggcacctcc cggtcgaggt tcgcgaccgc gaaatggcct ggggcgtgag
    11101 cgggtcgctc gtcaacctcg actcggcact cggccgtctc tcgctgaagc ggccgttcag
    11161 cgaaacgctg ccgcttccgc cggccttgtc ggaccaggat cgccggacac tcatcgagaa
    11221 cgccgtgctg tcggtgcccc aggatttcac cgaagccggg cgcgagaggc tgctggcggc
    11281 cgtcgagcgg gggagggcgc gcgtggaggc gctcggccct gcgacggacg agatcgaacg
    11341 cttggcagcc gacgtcgatc tggacgagtg gcgcgcggaa gcgctcgcct ggaccatcgc
    11401 caacgaacca tcgcggcggc tcgacttcct ctcgctcggc gaactggccc gggcgggagg
    11461 gcttgatccg cggggcgacg cgtcgctgga cgcgtggggg acgtccgagt atgcccgcga
    11521 cggcgcgctc gtgaaccgct acccgccgcg cctggacttg acgatcatgg cggggagacg
    11581 gggcgttcgg gcgacggcct cgttggtgcc cgacctcgcc atcgggctcg ccgagtcgtc
    11641 caagcggctg ggcctgccgg ccgccctgac gccggggctc ctcctcgtcg cgacccagcg
    11701 gttcatcgag accgtccagg tcgtccacgc ggacgactgg ttgacgatgg tgacgcacgc
    11761 caggcgggtg gcgcgcgaag gagtggatga ttacgttgcc ctgctgacgg tgtccggccc
    11821 cctcgtcacg gaggattgac cttgggatcg agcccgcacc ctctcgcggc ggcgctgtgt
    11881 gccgccgtcc tcatcgccgt tgccgcgccg ctggcgcaag cgcccgcgcc ggcggtcgtg
    11941 ttcgtctttc ccgccgacgg agactacgtg agcggcccgg tgacgctgaa agccgagctg
    12001 cggccgcacg accaggcgat caaaagcctc agcttgttcg cggatggcaa gctggtctgc
    12061 gcgctcgagc ggccgccgtt cgagtgcgac tgggacgcgg gccgcagggt ctccgagcac
    12121 gccctgcgcg ccgtagccgt cctccccgac gggcggcgcc tcgcccgcgg agttcgcacc
    12181 cggggcgtgc cgtacgccga ggccgtggac gtggacgtgg tgcacatcac ggccagcgtg
    12241 accgacggcg acggccgctt cgtaaaggga ctggctcgcc agcggttcaa ggtcttcgag
    12301 gacaacgccg cgcagaacgt gacctacttc gccgccgaga acgtcccgct cgagatcatc
    12361 gtggccgtgg acgtgagcgg cagcatggcc gacgcgatgc cgcagctgaa ggccgcgacc
    12421 aagaagttcc tctcggcgct gcggccggcc gaccgcgtga ccctgatcgg gttcaacgac
    12481 aacgtgttca cgctggcgcg gccaggcgtc gatctggccg cacgcctgcg cgccgtggat
    12541 cgccttgcgc cgtggggcgg caccgcgctc tacgacgtca tcgtccaggc ggtggaccag
    12601 ttggggcggc acgcgggacg gcgggtgctg gtggtgttca ccgacggcga agacctgaac
    12661 agccacgtgg cggtggaggt ggccgagggg cgcctcgagg ccagcgacgt ggtgctgtat
    12721 ccgattggcc aggggcgggc ggtggagggc gccaagtcgc tgaaggcgat cctggagcgg
    12781 ctcgcgcaga agaccggcgg gcgggcgttc ttcgaggagg ccgaccggct cgaggcggtg
    12841 ttcggcaaca tcctcgagga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 ncggccggtg cccggcctgc agcccgccga ttttcgggtc ctggtggacg gccggccccg
    13021 ccaggtcctg accgcggagt tcctctcgtc agcggcctcg cctgcggccg cagctgacgc
    13081 ggagcgcggc gcggaccgcg gcgagctcga cgccgtgttc gagagcgtct acgtcggcaa
    13141 ccaccgcgcg ggcggccagc gagccacgcc gtcgcggacc gtgatgattg cgatcgacca
    13201 aagtggcttc tcgctcgggg ccggccgcaa cgcggccacg acggcgcgcg gcctgctcga
    13261 cctgcttcac cccaacgatc gggtggggct cgccgtgttc ccccagccgg gagtcttcgt
    13321 ccggccaacc cgggatcacc agcgcgtgcg cctggcgctc ggccaggtca ccggcgcgcc
    13381 cgacccgttt ccgcgcagcg agatcagcct gaacgtcgcc gatgcgctgg cctggacgag
    13441 cggcgacacg atgtcgcgcg acctggtcgt gcagaagtac tgctcgcaca cggcagcaat
    13501 gacctgccag ccggagctcg accgggcggc cagccagatc atcagctacg cccagcgaca
    13561 gagcctgacc acgctgcagg ggctgggcga cgtgatcaac gccgcgaccg ctgacggcga
    13621 cccggtggcg gtggtcctgg tgagcagcgg cgtgtacgcc agcgggcgag ccacgcagct
    13681 gggcgtggac ggcgaggcgc ggacgctcgc gcgaatcgcc gcgtcgtcgc gcgcgatgct
    13741 gtacgcgatg tacgtcgaga cggggttcct cgaccgcgac tcgatggagc gggcgcgcac
    13801 ggcgggcttc tccagcgagg acggcgacat gcggctcgag gggttgcggc atctcgcggg
    13861 actctccggc ggcacggtca cgcgcgtcgt ctcggggagc gacgaaggtt tccggcgcgt
    13921 ctccctcgag ctgtccgcct actatctcct cggcgtggag ggcatgccgg gcgaccggga
    13981 cgggaatcgc caccgcctcg aggtgagcgt ggcgcggccg gggctgaccg tccgcagccg
    14041 ggacgagctg tacctggccc cgccgaggaa gaccacagcg gagcagagcg tcgtcgaggc
    14101 gctcgacgct cccggcctcg tcggcgacct gccgatccgc ctgtcgacgc agatgatgcg
    14161 cgagccgggc accgacaagg tgcggatggt gatcagcgcg gccgtcgggc gcgaggtggg
    14221 gcggccggcg ccggtgcgcg tcgggtacgt tctgagggga gccggctcga agggaacggc
    14281 ggcaggcagc ggggtccagg cgcgagagtt gccagtcgtt ggagatggtc ccgatgcgtc
    14341 gtggtcgttc atcgagaccg cgcaactgac ccccgggcgc tacctgctcc ggctggcggc
    14401 gattgacgag gaggggaggc tgggaagcgt gcagcaggtc gtcgacgcga tgctggtcgg
    14461 aggcgatgga ggcaccttca gcgatctgct gctcgtggat cctgcgcgca cggtcaaggg
    14521 gacgttcacg ccggccgccg acgggcgcat gacggccgac tcgctcgaag cgttcctcga
    14581 gatctatccc gggaggggcg caagcgtgcg ctcggtcgcc ttcgaggtgg acgatgcccc
    14641 gggcggagcg ccgctcatca cgcgatccgt cgtgcccgag cggagggagg acggccggct
    14701 ggttgcggga atcaacttcg agctgcgctc gttgccgccc ggcgtctacg ccctcgacgc
    14761 gcgaatcctg gacggagaac gtgttctcgg gcgggtgagc cgtcccttcc ttctggagcg
    14821 ccgttcggtg gggagcgccg gggcgcctcg cgccgcgctg gcgtttgccg caaccggagg
    14881 gctcgtccgc gcgttctcag ggcgggaagc gctccgcccc gacgcgcttg gctacttcct
    14941 gaaccgcatg tcggcatccg aggtcgcggc aaccggaacg gaggtggcgg aggcagcaga
//
LOCUS       DAOPQX010000050        14743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4793_length_14743_cov_4.066517, whole genome shotgun sequence.
ACCESSION   DAOPQX010000050 DAOPQX010000000
VERSION     DAOPQX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_14743_cov_4.066517"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PKK95_04460"
     CDS             <1..833
                     /locus_tag="PKK95_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC17496.1"
                     /translation="PSHEEALGNLQYGITSRKGITLLLGEAGTGKTTLLRKALTLRVH
                     GEPGAEGGDGWVYLNNPTLTRQEFLEALAEGFRLGPWAAASKVRLLRALHESLADRHR
                     RGLASALIVDEAQSLPHELLEELRLLVNIESDTEKLLPLVLAGQPELADRLNERGLRQ
                     LKQRVALRCRLRAFTLRETALYIATRIRLAGGDAGLVFTRDAVIAVHEAAEGIPRTIS
                     VICDNALLNGFAADRRPVDADIVREVCSDFDVGADAEPRPLAPRRLAFPPRAHIAREA
                     "
     gene            1095..2063
                     /locus_tag="PKK95_04465"
     CDS             1095..2063
                     /locus_tag="PKK95_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOC17497.1"
                     /translation="MKTHLIVALSLSLLSTAPAAAQAPATPPGPESTPTTSGADLAET
                     AEKAAAGRDYRIGSDDELTVNVMQAPELNRTTRVTEEGLISLPLVGSVQAGGLTSVEL
                     EAAIEEALARRYIKDPEVTVLVTEVRSRPVSVGGAVNRPGIQQARGTTRLLDVISLAG
                     GLRNDAGDTVVVARKTGDGRAEPIEIPLKPLLESRDPELNVTIHAGDTVTVRTAETVY
                     VVGAVKKPGAFAMRGNERLTVLQALALGEGLVPTAAQKDALVVRTGPDGGRQEIPVDL
                     AAVLRGKRPDVPLEAQDVLFVPTSGGKVAARAALDAFIRVISWRPF"
     gene            2108..4414
                     /locus_tag="PKK95_04470"
     CDS             2108..4414
                     /locus_tag="PKK95_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17498.1"
                     /translation="MSRIADVLKKAGDGQAGASHAEGSPEAAADGAGCPGNSVPWELE
                     DGRSTPRVRRDASGEQGLVRRAEGYVSPIPRAGGVLGRNPMPPPSVPPLRQYLDVLRR
                     RWRTALLILLVVVGGVGAGAALQEPVYRASGLLEIRRESTSAVPVDTLFSTERVTSDD
                     LETQYGILKSATLAQRVIEQLGRDGVGRRTEASAASQVGGFRKSLVVNPLRGSRLVEV
                     AYLSGDPQLAARTVNSVFDNYLQLRMEEAQRTAQWLENQLRDAQERLEESDRRLQAYI
                     RQHGLQVIETGKGEAAELANERLQALHEALATAQAERMDRQSAGEAARRAGARGGLDS
                     PVAQNLTMRLADLRREHAKLSSAFHEEYPSVKALNQQIAELERALAEESRSVVARTER
                     EYQAALRKEALLRRALEEQNAAVQDLGRRSAGSAGYEALRRELVTNQEQFAILNQKLK
                     EISVSAALKAQSVGVVDRARPPSRPSGTPLPVTLALALVVGVVVAAGGALLRDHFDTS
                     VRTAADVEAFLGVPALGAIPAVRVDPLPPGAADLRSWRRIDDRPGPQTPLAEAFATLR
                     NAVLLHDGAADSRVLLITSAQSEEGKTTVSVNLALSLARLEYRVLLVDANMRFPCIRR
                     ALGLPDGPGLVDYLDARVDWRAAVQAGVQPNLDVLGGGEPEKSPADLLSRPLMGSLLD
                     TAAREYDFVVIDSPAVLTHPADVHSLAAVADNVLFTVRHGWTPREVVALALSRLDRVS
                     GVVLNRSVSPEFGPRPEERQRRISALAS"
     gene            4497..5036
                     /locus_tag="PKK95_04475"
     CDS             4497..5036
                     /locus_tag="PKK95_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17499.1"
                     /translation="MPILRTWVGGGSGGSLPRPEPHAAGVEVPDTDEAIKRIAIEFER
                     ARRYERSVTVAVFSIEAPSPDAPGASRPEPAGHASAAKHLCPVVREAMRETDLVVCDP
                     ATRRCVVVMPEIGPEEGHRAVARMRDLCASRLECPVRGDLAVFPRDGWVFPDLVEVAE
                     KQAQQAAGMTLVTHEPLRS"
     gene            5104..5790
                     /locus_tag="PKK95_04480"
     CDS             5104..5790
                     /locus_tag="PKK95_04480"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOC17500.1"
                     /translation="MTMSVHAPGWRTGSLRHSEVLAPARPAPSPMYERVKRAIDLVVC
                     LAVLPAILVVLALCVVAIKIESPGKAFFVQMRTGRGGRRFRMYKLRTMVPNAAELKAK
                     YLHLNELTWPDFKITNDPRMTRVGRILRKTSLDELPQIFNVILGDMTLVGPRPTSFGA
                     ETYRLWHTARLEVKPGLTGLWQVSGRASLDFDDRLRLDIAYIRNRSLALDLRILVQTF
                     TAVLSGRGAN"
     gene            5792..6958
                     /locus_tag="PKK95_04485"
     CDS             5792..6958
                     /locus_tag="PKK95_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC17501.1"
                     /translation="MTGLAVWIFWISVACLLYVYAGFPLLVAVVGWLRRRKVRTAPVT
                     PRVSLIIAAYNEEAVIGERLENALALDYPPERLQILVAADGCTDATEAIAGCYADRGV
                     EVLSLPRQGKIRALNEAAARATGEILVFSDANIMCHPGALKALTRNFADPEVGGVAGC
                     TGYKLEEASESSGQGERLYWDYDTWLKRLESATGSIVSAHGGLYAVRRQLFRPVSDSA
                     VTDDFAISTGVIEEGFRLVFEPDARASEYAVPKAQREFRRRVRLMTRGLRGVILRRRL
                     LNPARYGFYAVVLFSHKVLRRLVPVPLVAIAAASAYLAPAHPFYLAVAASQALFYALA
                     ALGFLLRRAAAGRLKVVYIPFYYCMANVACGVALVQLLRGRRIELWQPQRQAVR"
     gene            6963..8510
                     /locus_tag="PKK95_04490"
     CDS             6963..8510
                     /locus_tag="PKK95_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC17502.1"
                     /translation="MNIARTAAQPLHILTTGLLAASAAGGIAVLVGQAGAPTVLVLML
                     GAVGAGVMLARPDWATLLTAFLLYVNLPAILTKEHGLPGPVAGGVILLFGVRLVHRLV
                     IERRPPRVDWTLGLMVAYLAFMLLSTLAAVDTSLALNRVFEFVAEGLLLYFLVINAVT
                     DLAMLRRVFWVLLAAAALVSTLCLYQDVTGSYTQEFGGLAHRNYEASDDSSNVEVVRR
                     RRTWDRAQGPVDEPNRFAQVLIVLVPLAMFLFRNAATPLQRLAAAGLGGLTLMGAGLT
                     LSRGGILTLALLALAMARIRWVRTLQLAAGAAVMVVVFSAVSPFFVSRMLSIVNAGHL
                     LGDDQFSHQQADGAMRGRLTEMLAALHVFMDHPVIGVGPSQFARYYFVDYAGNAGIKF
                     REINVPRRAHSLFFELGAEQGVLGLAGFSVLVGGLMWKLWRGRRRWLGRDLERADLAA
                     ALTLSLFAYLATGFFLHLSYQRYFWLLVALGSAALHVVTSREREPRPSADGRGGNRLA
                     AVGETTR"
     gene            8507..10738
                     /locus_tag="PKK95_04495"
     CDS             8507..10738
                     /locus_tag="PKK95_04495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17503.1"
                     /translation="MRGSLRILMYHRVLDAGSTAACSPSVISATPAAFDEQMRQVARR
                     YRALSLDEALECVRNGRSLPARAVLITFDDGCRDFGEVAWPILRRYKLPATVFVPSAF
                     PGHPARSFWWDRLHAAIEGTRRQALETPAGRLPLGTVAERRAAVGCLQRIVKRTPHAE
                     AMRMVDGWCRELGGQQPPPNEVLRWDELRALAADGATIGAHTRTHPALTQLSTEEARE
                     EIRQSAADVRREIGSCPPVFAYPFGDHDDRIVQVVREEGFELALTCLDGHNRLPGDDR
                     LRLRRIGITRRTTGAIFRLRMTPAGSALDRWRHGGRGGAHPAAARPVEPGEERPARVA
                     YVMSRFPKLSETFVLNEMAACAEQGIAVELYPLLRERQPVVHPEVDAWVRRAHYHPFV
                     SPAIVRSNWRFLREQPVRYAKLAATVLRETWRSPNFLAGAIGIFPKSVHFADLMRQQG
                     VTHVHAHFATHPALAAFIVHRLTGIPFSFTAHGSDLHVDRRMLDTKVAAAAFAIAVSS
                     YNREVIVRECGEGSRGKVHVVHCGVDPLFFGPRPDDRRHEGLFRVVCVASFEEVKGHR
                     YLVEACALLRARGVPVVCDLIGEGPLRRAIEARVAAAGLRQIVRFHGGLPRPEVSRLL
                     AAADAAVLASHPTRSGKREGIPVALMEAMASGLPVVATAISGIPELVQSGLTGFLVPP
                     RDPDALADALQRLAADPDLRQQMGTAGRARVMRDFDLRANAATLARMFTPARGDLRVR
                     VAS"
     gene            10758..>11232
                     /locus_tag="PKK95_04500"
     CDS             10758..>11232
                     /locus_tag="PKK95_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17504.1"
                     /translation="MLLTVTSREKDAGREDLTRRLEQYLVECAAASFPELSGAVRVAL
                     RRRELRAFTTLYEFALTAGATERRVLVKIPGNSARKDGQPGETAVARRPRRFPPVDPA
                     SRVRQECLVLNEMHRYFGSLGDPRFAAVRPVGHLDDVGAFLMELCPEPSLSRLMLA"
     assembly_gap    11233..11242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11243..11912
                     /locus_tag="PKK95_04505"
     CDS             <11243..11912
                     /locus_tag="PKK95_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOC17505.1"
                     /translation="RVPGARAARLRRACRNAGAWLRAWHEHPGLAHTVTRNTTRDEFV
                     GGICELTRYVAGIDGRAGWMDEVCERVAASALRTLPPELPSGAFHGDFAPRNVLAGVD
                     GRVTVIDTAARWRTPIYADLAYFLFSVDAARPQVYTGGLMFGQSAVDAWRAEFLRGYF
                     EDDPVPVGAIALFEVDALLDRWAAEAARVAAAGANKGGNRWRFRAWDRFLRARLRDRL
                     RELS"
     gene            complement(11952..13169)
                     /locus_tag="PKK95_04510"
     CDS             complement(11952..13169)
                     /locus_tag="PKK95_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOC17506.1"
                     /translation="MHATTRGGLAALISIAALTGVLAQQATPPGPPTNVRILSQQTGG
                     TGGGSGPRTGIWIGKSELDALPMSGTAWNELKAEADSSWTVQSIETQSGQVFNVQAMA
                     GALVYARLAPNASAGTYRAKVAQAIRNVMARPDQSSSCTAPNRNLGTWAISADLINLR
                     EYDPTLDTQFRAWLRRKLDVPYDSSPSTIRTQVARPNNQGAWACFAVTAVSRYLGDTA
                     TLNTIAVRMRRFLGDTSAPYSLRWDTSNQSWQANPGSQASWVGINPKGAKRDGHNFDG
                     IQPEDQRRGTPESYSASSFPNDFSSVRYNEVALGAYLGTVLMLERAGYRDLVDASDQA
                     LLRAARWIKYAADNYASKGYVYFRGFHEAAQPLVNYFYNAGMPEVKSRDQMEGREFGY
                     SWTFWTHAGKRLR"
     gene            13524..14582
                     /locus_tag="PKK95_04515"
     CDS             13524..14582
                     /locus_tag="PKK95_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC17507.1"
                     /translation="MPRSQPHQDLAWPPAPDDLAYLDLEQPEQPPPSTGFPGVDETVR
                     RAPERRAGHDPSPRKPEPFILPPARPQLPLVTGAKRFAGRGALALLAVCLLAALVVAV
                     LRLAPAGPAASPGDAGAPADTLDSTPPVAAEPPAPTTGSLAVRTEPAGAIVTVDGKRL
                     GASPVIVPSIGPGEHAVVIQHAGGTYRHAARVAAGETVSLVVPLPAAAEPAAAPAAEG
                     SIQVRAPFELRVTKEGRLLGTSEMDRIPLDAGTHTVQLTNEAVGYQASRTVRVSAGRT
                     TIVEAEVPEEPVAINAIPWAEVTIDGRPVGQTPLGALTVPIGPHLVVLSHPRFGEKTV
                     RTTVRAGEPARISVDMRR"
BASE COUNT         2019 a         5169 c         5330 g         2215 t
ORIGIN      
        1 caccgtccca tgaggaggcc ctcgggaacc tgcaatacgg catcacttcc cgaaagggga
       61 tcaccctcct cctcggcgag gcggggacgg ggaagacgac gctgctgcgc aaggcgctca
      121 cgctgcgggt gcacggggaa ccgggcgccg agggcgggga tggatgggtc tacctgaaca
      181 accccaccct cacgcggcag gagttcctcg aggcgctggc cgaggggttt cgcctcggcc
      241 cttgggcggc agcttcgaag gtgcgcctgc tgcgcgcgct gcacgagtcg cttgccgatc
      301 ggcacaggcg ggggctggcc agcgccctga tcgtggacga ggcgcagagc cttccgcacg
      361 aactgctcga ggagctgcgg ctgctggtga acatcgagtc ggacaccgag aagctgctgc
      421 cgctggtgct cgccgggcag cccgagctgg ccgaccgcct caacgagcgc ggcctgcggc
      481 aattgaagca gcgcgtcgcg ctccgctgcc ggctgcgcgc cttcacgctg cgcgagacgg
      541 cgctctacat cgcgacccgt atccgcctcg cgggaggaga cgccggcctc gtcttcacgc
      601 gcgacgccgt catcgccgtc cacgaggcgg ccgagggcat tccgcgcacc atcagcgtca
      661 tctgcgacaa cgcgctgctc aacggcttcg ccgccgacag gcggccggtc gacgccgaca
      721 tcgtccggga agtctgctcc gacttcgacg tcggggccga tgcggaacct cgtcccctcg
      781 cccctcgccg cctggccttc ccgccccgcg cccacatcgc gcgcgaagcc tgatccggcg
      841 aaccggcccg gccgcttcag gcgcccggcc ccggggccgc caaccacccg gccgcctcgg
      901 ccttccgttt ccgcctcgag gcgacggacc atcttgtcat ccagaacgcg agtgcggact
      961 ccttccgagg ggccctccgt gcgtcctaag accgtcggga ctcaggtcac cccaaaaacg
     1021 ggaggcgcgt ctcatcaccg caccgaccct cgcatgctga aatttcctgt cgctttgtca
     1081 ttggagccac tgccgtgaag acgcacctga tcgttgcctt gtcgctgtcg ctgctctcaa
     1141 cggccccggc tgccgcgcaa gccccggcga ccccccctgg tcccgaatcg accccgacca
     1201 cctcgggtgc ggacctggcg gagacggcgg agaaagcggc ggcgggccgc gactaccgga
     1261 tcggctccga cgacgagttg acggtcaacg tcatgcaggc tcccgagctc aatcggacga
     1321 cgcgggtcac cgaggagggg ctgatctcgc tgccgctggt cggttcagtg caggccggcg
     1381 gtctcacctc ggtggagctg gaggcagcga ttgaagaggc cctcgcccgg cgctacatca
     1441 aggacccgga ggtcaccgtc ctcgtcaccg aggttcggag ccgaccggtc tcggtcggcg
     1501 gcgccgtgaa ccgtccgggc atccagcagg cgcgcgggac gacacgcctg ctcgacgtga
     1561 tctcgctggc cggcggcctt cgcaacgacg cgggcgacac cgtggtcgtc gcccgcaaga
     1621 ccggcgatgg ccgggccgaa ccgatcgaga tcccgctgaa gcccctgctc gagtcgcgcg
     1681 accccgagct gaacgtgacg attcacgcgg gcgacaccgt cacggtcagg acggcggaaa
     1741 cggtgtacgt cgtcggcgcg gtgaagaagc ccggtgcgtt cgcgatgcgg ggcaacgagc
     1801 gcctcacggt cctgcaggcg ctggcgctcg gcgagggact ggtgccgacc gctgcgcaga
     1861 aggacgcgct ggtggtgcgc acggggcccg acggcgggcg gcaggagatc cccgtcgacc
     1921 tcgcggcggt gctgcgggga aagcgccccg acgtgccgct cgaggcgcag gacgtccttt
     1981 tcgttccgac gagcggcggc aaggtcgcgg cgcgcgcggc gctcgacgcg ttcatccgcg
     2041 tgatctcgtg gcggccgttc taggctcccg ggtggacgcg cggccaacag gcagcggtga
     2101 aattcacatg agtcgaatag cagacgtgtt gaagaaggcg ggggacggcc aggccggggc
     2161 cagccacgcg gagggctctc ccgaggctgc ggccgacggg gccggctgtc cgggcaactc
     2221 cgtgccgtgg gaactcgagg acggccggag cacaccccgc gtgcggcgcg atgccagtgg
     2281 agagcaaggg ttggtccgcc gggccgaggg gtacgtatcg ccgattccga gagccggcgg
     2341 cgtcctcggg cgcaacccga tgccgccgcc ctccgttccc ccgctgcgcc agtacctcga
     2401 cgtcctgaga cggcgctggc ggacggcgct gctcatcctc ctggtggtgg tcgggggcgt
     2461 cggcgcgggc gcggcgctgc aggagccggt ctaccgcgcg agcgggctgc tcgagatccg
     2521 gcgcgagagc acgtcggccg tgccggtgga cactctgttc agcaccgagc gggtgacgag
     2581 cgacgatctc gaaacgcagt acgggatcct caagagcgcg acgctcgcgc agcgcgtgat
     2641 cgagcagttg ggccgcgacg gcgtgggccg caggaccgag gcgtcggcgg cgtcgcaggt
     2701 gggggggttc cgcaagtcgc tggtggtcaa cccgctgcgg ggaagccgcc tggtcgaagt
     2761 ggcgtacctc tcgggcgacc cgcagctggc ggcccgcacg gtgaactcgg tcttcgacaa
     2821 ctacctgcag ctgcggatgg aagaggcgca gcgcaccgcg cagtggctcg agaaccagct
     2881 gcgcgacgcg caggagcgtc tcgaggaatc cgaccgccgc ctgcaggcct acatccgcca
     2941 gcacgggctc caggtcatcg agaccggcaa gggcgaggcg gccgagctgg cgaacgaacg
     3001 gctgcaggcg ttgcacgagg ctttggccac cgcccaggcc gagcggatgg atcgccagtc
     3061 ggccggagaa gcggcgcggc gcgccggcgc gcgtggcggc ctcgacagcc cggtggcgca
     3121 gaacctgacg atgcgcctgg ccgacctgcg gcgcgagcac gcgaagctgt cgtcggcgtt
     3181 ccacgaggag tacccgtcgg tgaaggcgct caaccagcag atcgccgagc tggagcgcgc
     3241 gctcgccgag gagagccgat cggtcgtggc ccgcaccgag cgcgagtacc aggcggcgct
     3301 gcgcaaggag gcgctgctgc gccgggcgct cgaggagcag aacgccgcgg tacaggacct
     3361 gggccgccgt tcggcgggaa gcgcgggata cgaggcgctc aggcgcgagc tggtcaccaa
     3421 ccaggagcag ttcgcgattc tcaaccagaa gctgaaggag atcagcgtct cggcggcgct
     3481 caaggcgcag agcgtcggcg tggtggaccg ggcccggccc ccgtcacgcc cctcgggcac
     3541 gccgctgccg gtgacgctcg cactggcgct ggtcgtcggg gtggtcgtcg ccgccggagg
     3601 cgccctgttg cgcgaccact tcgacacgag cgtccgcacc gcggccgacg tcgaggcgtt
     3661 cctcggcgtc ccggcgctcg gcgccatccc cgcggtccgg gtcgatccgc tgccacccgg
     3721 cgccgcggac ctgcggtcgt ggcgccggat cgacgaccgc cccgggccgc agacgccgct
     3781 cgccgaggcg ttcgccacgc ttcggaacgc ggtgctcctg cacgacggcg ccgccgactc
     3841 gcgcgtgctg ctcatcacca gcgcccagtc ggaagaaggg aagaccaccg tctcggtcaa
     3901 cctggcgctc tcgctggccc ggctcgagta ccgcgtcctg ctggtggacg cgaacatgcg
     3961 cttcccgtgc atccggcgcg cgctcggcct gccggacggc cccggcctgg tggactacct
     4021 cgacgcgcgc gttgactggc gcgccgcggt gcaggccggc gtgcagccga acctcgacgt
     4081 cctcggcggc ggcgagccgg agaagagccc ggcagacctg ctctcgcgtc cgctgatggg
     4141 cagtctgctc gacacggccg cccgcgagta cgacttcgtc gtcatcgact cgccggccgt
     4201 gctcacgcac ccggccgacg tccactcgct cgcggccgtc gcggacaacg tgctgttcac
     4261 ggtgcgtcac ggatggacgc cgcgcgaggt ggtggcgctt gctctctcgc gcctcgaccg
     4321 ggtgagcggc gtcgtgctga accggtcggt gagccccgag ttcgggccgc ggccggagga
     4381 acgtcagcgc cggatctcgg cgctggcatc ctgatcccgg cgcggcgact tgcgccgcgc
     4441 tcgcttcacg gaggagacat cggatgtggt cctggctgtc tggtggctgg tcccgtttgc
     4501 cgatcctgcg aacgtgggtc ggcggcgggt cgggcggctc gcttccacgc cccgagccgc
     4561 acgcggcggg cgtcgaggtt cccgacacgg acgaggcaat caagcggatt gcgatcgagt
     4621 tcgagcgggc ccgccgttac gagcgaagcg tgaccgtcgc cgtcttctcg atcgaagctc
     4681 cgtcgccgga cgctccgggc gcctcgcggc cggagccagc cggccacgcc agcgcggcca
     4741 agcacctgtg cccggtcgtg cgcgaggcca tgcgggaaac cgatctcgtg gtctgcgacc
     4801 cggcgacgcg gcgctgcgtg gtggtgatgc ccgagatcgg gcccgaggaa gggcaccgcg
     4861 cggttgcccg gatgcgcgat ttgtgcgcgt cgcggctcga gtgcccggtg cgaggcgatc
     4921 tcgcggtgtt tccccgcgac ggctgggtgt ttcccgacct ggtggaggtc gccgagaagc
     4981 aggcgcaaca ggcggccggg atgacgttgg tcacgcacga acccctgcgg agctgatggc
     5041 ggtgtcgaac gagcgggctg acgcgcccgc ggcccggaat cgcgccggga cggaggaacg
     5101 cgtatgacga tgtctgtgca cgcccccggc tggagaaccg ggtcgctgcg ccactcggaa
     5161 gtgctggcgc cggcgcggcc cgcgccaagc ccgatgtacg agcgcgtcaa gcgggcgatc
     5221 gacctggtgg tctgcctcgc ggtcctgccg gccatcctgg tggttctcgc gctgtgcgtg
     5281 gttgccatca agatcgagtc gcctgggaag gcgttcttcg tccagatgcg caccgggcgc
     5341 ggcggccggc gcttccggat gtacaagctg cggacgatgg tgccaaacgc ggccgagttg
     5401 aaggccaagt acctgcacct gaacgagctg acctggcccg atttcaagat caccaacgac
     5461 ccgcggatga cgcgcgtggg gcggatcctg cggaagacga gcctcgacga actgccgcag
     5521 atcttcaacg tgatcctcgg agacatgacg ctcgttggcc cccggccgac ctcgttcggc
     5581 gccgagacct atcgcctgtg gcacaccgcg cgcctcgagg tgaagccggg gctcacgggc
     5641 ctctggcagg tgtcgggccg tgcgagcctc gacttcgacg accgcctgcg gctcgacatc
     5701 gcttacatcc gcaaccgcag cctagcgctc gacctgcgga tcctggtgca gaccttcacg
     5761 gccgtcctgt ccggacgcgg agccaattga gatgaccggc ctggccgtct ggatcttctg
     5821 gatttcggtg gcgtgcctcc tctacgtgta tgcgggcttc ccgctgctgg tggcggttgt
     5881 cggctggctc cggcgccgga aggtgcggac agcaccggtg acgccgcgcg tcagcttgat
     5941 catcgccgcc tacaacgagg aggccgtgat cggcgagcgc ctcgagaacg cgctcgcgct
     6001 cgactacccg ccggagcgcc tccagatcct ggtcgccgcc gacggctgca ccgacgcaac
     6061 cgaggccatc gcggggtgct acgccgaccg cggcgtcgag gtgctgtcgc tgccgcgcca
     6121 ggggaagatc cgcgccctca acgaggcggc cgcgcgagcc accggggaga tcctcgtctt
     6181 ctccgacgcg aacatcatgt gccaccccgg ggcgctcaaa gccctcacgc gcaacttcgc
     6241 cgatcccgag gtcggcggcg tggcgggatg caccgggtac aagctcgagg aggccagcga
     6301 gtcgagcggc cagggcgagc gtctgtactg ggattacgac acctggctca agcgcctcga
     6361 gagtgccacc ggcagcatcg tgtcggctca cggcggcctg tacgccgtgc gccggcagct
     6421 cttccgcccg gtgtccgact ccgcggtcac cgacgacttc gccatctcga ccggggtgat
     6481 cgaggagggg ttccgcttgg tcttcgagcc cgacgcccgg gccagcgagt acgccgttcc
     6541 gaaggcccag cgcgagttca ggcgccgcgt acgcctgatg acacgcggcc tgcgcggcgt
     6601 gatccttcgc cggcgcctgt tgaaccccgc ccgttacggc ttctacgccg tggtcctgtt
     6661 ctcccacaag gtcctgcgcc ggctcgtacc cgtgccgctg gtcgccatcg ccgccgcgag
     6721 cgcgtacctc gccccggccc acccgttcta cctggcggtc gcggcgtccc aggccctgtt
     6781 ctacgcactg gcggcgctcg ggttcctcct gcggcgcgcc gcggcggggc gcctgaaggt
     6841 ggtgtacatc ccgttctact actgcatggc caacgtggcc tgcggggtgg cgctcgtgca
     6901 gctgctgcgg ggccggcgga tcgaactctg gcagcctcag cggcaggcgg tgcgctgacg
     6961 caatgaacat cgcccgcacg gccgcacaac cgctgcatat cctgacgacc gggctgctgg
     7021 ccgcgagcgc cgccggcggc atcgcggtgc tggtcggcca ggccggcgct cccacggtgc
     7081 tcgtcctgat gctgggggcc gtcggcgccg gcgtgatgct ggcccgcccc gactgggcga
     7141 cgctgctgac ggcctttctc ctctacgtga acctgcccgc gatcctcacc aaggagcacg
     7201 gcctgccggg gcccgtcgcc ggcggtgtca tcctgctgtt cggggtgcgg ctggtgcacc
     7261 ggctggtgat cgaacggcgg ccgccgcgcg tcgactggac gctcggcctg atggtggcgt
     7321 acctcgcgtt catgctgctg tccacgctgg cggccgtgga cacgtcgctc gcgctgaacc
     7381 gcgtcttcga gttcgtcgcg gaaggcctgc tgctgtactt cctcgtcatc aacgcggtga
     7441 ccgacctcgc gatgctgcgg cgcgtgttct gggtgctcct cgccgcggcg gccctggtga
     7501 gcacgctctg tctctaccag gacgtcacgg ggtcgtacac gcaggagttc ggcggcctcg
     7561 cccaccgcaa ctacgaggcg agcgatgact cgtcgaatgt cgaggtggtg cggcgccgcc
     7621 gcacgtggga ccgtgcgcaa gggcccgtgg acgagccgaa ccgcttcgcg caggtcctca
     7681 ttgtcctggt gccgctcgcg atgttcctct tccgcaacgc cgcaaccccc ctgcaacgcc
     7741 tggcggcggc cggccttgga gggttgacct tgatgggcgc cggcttgacg ctgtcgcgcg
     7801 gggggatcct cacgctcgcg ctgctcgccc tcgccatggc gcggatccgc tgggttcgca
     7861 ccttgcagct ggccgctggc gcggcggtga tggtcgtcgt cttctccgcc gtctccccct
     7921 tcttcgtgtc gcggatgctg agcatcgtca acgcgggcca cctgcttggc gacgaccagt
     7981 tcagccacca gcaagccgat ggggcgatgc gcgggcggct gacggagatg ctcgccgcgt
     8041 tgcacgtctt catggaccac ccggtgatcg gcgtcggccc gagccagttc gcccggtact
     8101 acttcgtcga ctacgccggc aacgccggca tcaagttccg cgagatcaac gtcccccggc
     8161 gcgctcacag cctgttcttc gagctcgggg ccgagcaggg cgtgctcggc ctcgccgggt
     8221 tctccgttct ggtcggcggg ctgatgtgga agctctggcg ggggcggcgg cggtggctgg
     8281 ggcgggatct cgagagggcc gaccttgcgg ccgcgctcac gctgagcctc ttcgcgtacc
     8341 tggccaccgg cttcttcctg cacctgtcct atcaacgcta cttctggctc ctggtggccc
     8401 tcggaagcgc cgcgctccac gtcgtgacgt cgcgggagcg agagccgcgc ccgagcgctg
     8461 acgggcgagg cgggaaccgc ctcgcggcgg tcggggagac gacgagatga gaggttccct
     8521 ccgcatcctc atgtatcacc gcgttctcga tgccgggagc accgccgcct gcagcccgtc
     8581 ggtgatcagc gccacgccgg cggccttcga cgagcagatg cggcaggtcg cccgtcgcta
     8641 ccgggccctg tcgctggacg aggcgctcga gtgcgtccgc aacggccgct cccttcccgc
     8701 acgcgcggtt ctcatcacct tcgacgacgg ctgccgggac ttcggcgagg tggcgtggcc
     8761 gatcctccgc cggtacaagc tgcctgccac ggtcttcgtg ccaagtgcgt tccccggcca
     8821 ccccgcccgg tcgttctggt gggaccgcct gcacgccgcg atcgaaggca cgaggcggca
     8881 ggcgctggag actcccgccg gacggctgcc cctcggcacc gtggcggagc ggcgcgcagc
     8941 ggtcggctgc ctgcagcgga tcgtcaagcg gacgccgcac gccgaggcga tgcggatggt
     9001 ggacgggtgg tgccgggagc tgggggggca gcagccgccc cccaacgagg tcctgcgctg
     9061 ggacgaactg cgcgcactcg cggctgacgg cgcgaccatc ggggcccata cccgcacgca
     9121 cccggccctg acgcagctct ccaccgagga ggcgagggaa gagatccggc agtcggcggc
     9181 cgacgtgagg cgcgaaatcg gcagctgccc gccggtattc gcctatccct tcggcgacca
     9241 cgacgaccgc atcgtccagg tggtgcgcga ggaaggcttc gagctggcgc tgacgtgcct
     9301 cgacggccac aaccggctcc ccggggacga ccggctgcgg ctgcgccgca tcgggatcac
     9361 gcgccggacg accggggcga tcttccgcct gcgaatgacc cccgccgggt cggccctcga
     9421 ccggtggcgg cacggcggcc gtgggggggc gcatcccgcg gcggccagac cggtcgagcc
     9481 tggggaggag cggccggcca gggtggcgta cgtcatgtct cgcttcccga agctgagcga
     9541 gaccttcgtc ctcaacgaga tggcggcgtg cgccgagcag gggatcgccg tggagctgta
     9601 cccgctgctg cgcgagcggc agccggtcgt gcaccccgag gtggacgcgt gggtccgccg
     9661 cgcgcactat catcccttcg tctcgccggc catcgtgcgg tccaactggc ggttcctgcg
     9721 cgagcaaccg gtccgttacg cgaaactggc cgccacggtc ctccgcgaga cctggcggag
     9781 cccgaacttc ctcgccgggg ccatcggcat cttcccgaag tccgtgcact tcgccgacct
     9841 catgcggcag cagggcgtga cgcacgtgca cgcccacttc gcaacgcacc cggcgctcgc
     9901 ggcgttcatc gtgcaccgcc tcaccgggat cccgttcagc ttcaccgccc acggttccga
     9961 tctccacgtc gaccggcgga tgctcgacac gaaggtcgcg gcggccgcgt tcgcgatcgc
    10021 agtctcgtcg tacaaccggg aagtgatcgt ccgcgagtgc ggggagggca gccgcgggaa
    10081 ggtgcacgtc gtgcactgcg gagttgaccc gctgttcttc gggccgcgtc ccgacgaccg
    10141 tcggcacgag gggctgttcc gcgtcgtgtg cgtggcgtcg ttcgaggagg tgaagggcca
    10201 ccgctacctg gtggaggcct gcgcactgct gcgggcgcgg ggtgtgccgg tggtgtgcga
    10261 tctgatcggg gaggggccgc tccggcgggc catcgaagcg cgcgtggcag cggccggcct
    10321 gcggcagatt gtccgctttc acggcgggct cccgcgcccg gaggtctcac ggctcctcgc
    10381 cgcagcggac gccgcggtgc tcgccagcca cccgacccgc tcggggaagc gggaggggat
    10441 cccggtcgcg ctgatggagg caatggcgtc cggccttccg gtggtggcga cggcgatcag
    10501 tggaatcccc gagctggtcc agtcggggtt gacggggttc ctggtaccgc cgcgcgaccc
    10561 ggacgcgctt gccgacgcgc tgcagcgcct cgcggccgac ccggatctcc gccagcagat
    10621 ggggacggcg gggcgggcgc gagtgatgcg cgacttcgac cttcgcgcga atgccgccac
    10681 gctcgcgcgg atgttcaccc ccgcccgcgg ggacctgcgc gttcgggtcg cgagctgagc
    10741 acgagggagg gatcgaggtg ctgctgactg tcacgagccg tgagaaggac gccggcaggg
    10801 aagacctcac ccgacggctg gagcagtacc tggtggagtg cgcggccgcc tcgttcccgg
    10861 agctctcggg cgcggtccgc gtggcccttc ggcgccggga actgcgtgcg ttcacgacgt
    10921 tgtacgagtt cgccctgacg gccggcgcca ccgagcgccg ggtgttggtg aagatcccgg
    10981 gcaactccgc gcgcaaggat ggtcagcccg gcgagacggc tgtggccagg cggccgcgcc
    11041 ggttcccgcc ggtggacccg gcctcgagag tgcggcagga gtgcctggtg ctgaacgaga
    11101 tgcaccggta cttcggcagc ctcggcgacc cacggttcgc ggcggttcgc ccggtgggtc
    11161 atctcgacga cgtcggggcc ttcctgatgg agttgtgccc ggagccgtcc ctcagccgcc
    11221 tgatgctggc ggnnnnnnnn nnccgcgtgc ccggcgcccg cgcggcgcgc ctgcggcgcg
    11281 cgtgccggaa cgcgggcgcc tggctgcgtg cgtggcacga gcacccggga ctggcgcaca
    11341 cggtgacgcg caacacgacc cgtgacgagt tcgtcggggg gatttgcgag ctcacccgat
    11401 acgttgccgg aatcgacggc agggccgggt ggatggacga ggtctgcgag cgggtggcgg
    11461 cctcggcact tcgcaccttg ccacccgaac tgccgagcgg cgccttccac ggggatttcg
    11521 cgccgcggaa cgtcctcgcc ggagtcgacg ggcgcgtaac ggtgatcgac acggccgcac
    11581 gctggcgcac gccgatctac gccgacctcg cgtacttcct gttctcggtg gacgcggcac
    11641 ggccgcaggt ctacaccggc ggcctgatgt tcgggcagtc cgccgtggat gcctggcgcg
    11701 ccgagttcct gcgggggtac ttcgaagacg acccggtgcc ggtcggcgcg atcgcgctgt
    11761 tcgaggtgga tgcgctgctc gaccgctggg cggcggaggc cgcgcgagtg gcggcggctg
    11821 gcgcgaacaa gggcgggaac cggtggcggt tccgcgcctg ggatcggttc ctgagagcgc
    11881 gcctgcgcga caggttgcgg gaactctcct gagaccgggg ccggcccgcc ctggagccgg
    11941 ccccgggaag gttaccgcag ccgcttgccg gcgtgggtcc agaacgtcca ggagtagccg
    12001 aactcccggc cttccatctg atcccgcgac ttaacctcgg gcatgccggc gttgtagaag
    12061 tagttgacca agggctgcgc cgcctcgtgg aaaccgcgga agtagacgta tcccttcgag
    12121 gcgtagttgt cggcggcgta cttgatccag cgcgccgccc gcagcagcgc ctggtccgac
    12181 gcgtcgacta ggtcccggta gccggcgcgt tcgagcatga ggacggtgcc gaggtaggcg
    12241 ccgagcgcga cctcgttgta gcggacgctc gagaagtcgt tcgggaagct cgaggccgag
    12301 tagctttccg gtgtgccgcg gcgctgatct tcaggctgga tgccgtcgaa gttgtgcccg
    12361 tcacgcttcg cgcccttggg gttgatcccc acccacgacg cctggctgcc cgggttcgcc
    12421 tgccacgact ggttcgacgt gtcccagcgc aggctgtacg gcgcggaggt gtcccccagg
    12481 aagcggcgca tgcggaccgc gatggtgttg agcgtcgccg tgtcccccag gtagcggctc
    12541 accgcggtga ccgcgaagca cgcccacgcg ccctggttgt tcggccgcgc gacctgcgtg
    12601 cggatggtgg acgggctgct gtcgtacggg acgtcgagct tgcggcgcag ccacgcccgg
    12661 aactgcgtgt cgagcgtcgg gtcgtactcc cgcaggttga tgaggtccgc gctgatcgcc
    12721 caggtcccca ggttgcggtt gggcgccgtg caggacgacg actggtccgg gcgggccatg
    12781 acgttgcgga tggcttgcgc caccttcgcc cggtaagtcc cggcgctggc gttcggagcc
    12841 agccgcgcgt agacgagcgc accggccatc gcctggacgt tgaagacctg gccactctgg
    12901 gtctcgatcg actgcaccgt ccagctcgag tcggcctcgg ccttcagttc gttccaggcc
    12961 gtgccgctca tcggcagggc atccagctcg gacttgccga tccagatccc ggtgcgcggt
    13021 ccggatccgc cgcccgtccc gccggtctgc tggctgagga ttcgcacgtt ggtcggtggg
    13081 ccggggggcg tggcctgctg ggccagtaca ccggtgagcg cggcgatcga aatgagtgcg
    13141 gcaaggccgc ccctggtggt cgcgtgcact gtgaacgtct cccttgttct ggccgtttcc
    13201 caaattgatg agctgtgcgc gagcaccgag aaacgggatg tccgagggtg tgcgagcggc
    13261 gcgaggaacg tccggcgtgg gggacgagcg ccaggacggc cgctcgccgg ggagcgtgcg
    13321 aaagtcgggc cgggcacact gccgtcatca tccgggtcgg attcttccgg aaaccgggat
    13381 ccgctgacgg atccgggaac ccggccccct gggctcacac gccaactttg accgcacgat
    13441 cctatttctg ttgctgacct tggcggcggg aaaatgggat ccccgtccca tggtctgcca
    13501 gctagcggcg tttacagtgg tccatgcccc gcagccagcc ccaccaggac ctcgcctggc
    13561 ctccggcacc cgacgacctg gcctacctgg atctcgagca gcccgaacaa ccgccgcctt
    13621 caaccgggtt cccgggcgtt gacgaaaccg tcagacgcgc gcccgaacga cgcgcgggcc
    13681 acgacccctc tccgcgaaag cccgaaccct tcatcctgcc cccggcccgc ccccagctcc
    13741 cgctggtgac gggcgcgaag cggtttgcag ggcgcggagc gctcgccctg ctcgccgtct
    13801 gcctgctggc cgcgctggtg gtggcggtgc tgcgactggc tcccgccgga ccggccgctt
    13861 cccctggaga cgccggtgcc ccggcggaca cgctcgactc gacgccgccc gtggccgcag
    13921 aaccgcccgc cccaaccacc ggctcgctgg ccgtgcgcac cgaaccggcc ggcgccatcg
    13981 tcaccgtgga cggcaaacgc ctcggcgcct cgccggtgat cgttccgtcg atcgggccag
    14041 gcgagcatgc ggtggtgatc cagcacgcgg gaggcaccta ccggcatgcc gcgcgcgtcg
    14101 ctgccgggga aacggtgtcg ctcgtcgtcc cgctgccggc ggcagccgag cccgcggcgg
    14161 cgcctgccgc cgagggttcg atccaggtcc gcgccccctt cgagcttcgc gtgacgaagg
    14221 agggccgact gctcggcacg agcgagatgg accgcatccc gctcgacgcc ggcacccaca
    14281 cggtgcagct caccaacgag gcggtcggct accaagcgag ccgcaccgtc cgggtttcgg
    14341 ccgggcgcac gacgattgtc gaggcggagg tgccggaaga gcccgtggcc atcaacgcga
    14401 tcccctgggc tgaggtcacc atcgacgggc ggccggtggg gcagaccccg ctcggcgcgc
    14461 tcacggtgcc gatcggcccc cacctggtgg tgttgagcca cccgcgattc ggggagaaga
    14521 ccgtccggac gacggtcagg gccggggagc cggcgcggat cagcgtggac atgcggcggt
    14581 gacgccccgg gcaggccgcg ggagacccat gcagaaacgg ggctaaagcc cctactagta
    14641 caggaacatg ggcttgccga gcatgtcgcg cacgcgcggc cggtactcgt gggtgtcgta
    14701 cagggcggtt acgtcgacgc gcttcttgcc ctggatcacg agg
//
LOCUS       DAOPQX010000051        14708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4807_length_14708_cov_4.508974, whole genome shotgun sequence.
ACCESSION   DAOPQX010000051 DAOPQX010000000
VERSION     DAOPQX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4807_length_14708_cov_4.508974"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKK95_04520"
     CDS             complement(<1..334)
                     /locus_tag="PKK95_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17508.1"
                     /translation="MPIWLATLTMTATWVDGGYLLGTVEGTFKSSVALGVQGGLCFGI
                     SLVLGGVFFARRMRALEFTTLIDPFESRFGPRWAAVLMLPALAAELFWSAELLVALGS
                     TLGVMAGIG"
     gene            complement(568..1065)
                     /gene="rplI"
                     /locus_tag="PKK95_04525"
     CDS             complement(568..1065)
                     /gene="rplI"
                     /locus_tag="PKK95_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOC17509.1"
                     /translation="MEVILREHVDNLGRRGDVVKVANGYARNYLLPRKLALPVTEANR
                     KQIERERKIAEVREAQEKQDAEAVAARMGEVELVFARRVGENETLYGSVTGADIAEAL
                     AGKQFEVEKRRVVLAEPLKQLGEFDVPVKIHREVTATVKVKVVPLEGQAAPAPAPAAE
                     PSEPQ"
     gene            complement(1079..1291)
                     /gene="rpsR"
                     /locus_tag="PKK95_04530"
     CDS             complement(1079..1291)
                     /gene="rpsR"
                     /locus_tag="PKK95_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOC17510.1"
                     /translation="MFRRRKVCKFCAEKIDYIDYKDVRLLMPFVPDRGKILPRRISGV
                     CAPHQRALQTAIKRARQLALVPYVTD"
     gene            complement(1378..1800)
                     /gene="rpsF"
                     /locus_tag="PKK95_04535"
     CDS             complement(1378..1800)
                     /gene="rpsF"
                     /locus_tag="PKK95_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOC17511.1"
                     /translation="MSTSRQYELIYIVSPDATEQEVADLHAQVEASVARFAGELVKTE
                     NWGRRKMAYEIGSHKEGIYVLELINGSGELMKEIERRLRVSDRIMRYLVVRVDEELRA
                     AERARARRKAVARPPQGGGEGAEAPAAGTDGREDAEVL"
     gene            complement(1890..2504)
                     /gene="pth"
                     /locus_tag="PKK95_04540"
     CDS             complement(1890..2504)
                     /gene="pth"
                     /locus_tag="PKK95_04540"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOC17512.1"
                     /translation="MPLKLIVGLGNPGRRYRDTRHNVGFAVVDELARRHDAGFEGAPA
                     DALMARARRLGPDGAILAKPLTMMNLSGFAVSELSRYFRVAPGDLLVVADDVALPLGR
                     LRARPRGSDGGHKGFRSIIEQLGNGEFARLRVGVGRGDARRDLADHVLGGFDPEERPQ
                     IAEAIGRAADAAEVFVLEGIEAVMNRFNADPLAREDAPGATPER"
     gene            complement(2521..3222)
                     /locus_tag="PKK95_04545"
     CDS             complement(2521..3222)
                     /locus_tag="PKK95_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOC17513.1"
                     /translation="MATSGEKDGLQNMEAVLEAVKRDRFGKNEAGRLRREGHLPGVLY
                     GGTAQRDALPIAVEPKALWRILHSETGANTLITLRLEGESPRVMVKEYQLDPVTHQLL
                     HADFYRVAMDKAITVTVPVTLKGEAPGVKQQGGLLDFVHREVEVECLPGDIPEAIEVD
                     ISGLMLNQSLRLRDVVESVKWKPVSDLDLMLVHVVAQKAEAEPAAAETEAAAAPAEPE
                     VIKKGKAEKEGEAEK"
     gene            complement(3304..4254)
                     /locus_tag="PKK95_04550"
     CDS             complement(3304..4254)
                     /locus_tag="PKK95_04550"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOC17514.1"
                     /translation="MRMAFAELKVFSGSAHPELAREIAGHLGLPLGQARIGRFPDSEV
                     SFQIEENIRGTDVFVVQPTSTPVDQHLIELLIMIDAFRRSSAARITAVIPYYGYARQD
                     RKDKPRVPISAKLVANILSAAGANRVLTMDLHKAQIQGFFDIPVDHLFAAPVIIDYLA
                     RQRFPNMTIVSPDAGGAERARAYAKRLGAGLAVIDKRRSETGAAEVMNVIGDVAGRTC
                     ILQDDIVDTAGTMQKAATALVENGAERVLACAVHGVLSGPAIGRIEQSPIEKLIVTNT
                     TPLGPEKSRCPKIVVLSVARLLAQAIRSIHEESSVSSLFV"
     gene            complement(4311..4384)
                     /locus_tag="PKK95_04555"
     tRNA            complement(4311..4384)
                     /locus_tag="PKK95_04555"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4350..4352),aa:Gln,seq:ttg)
     gene            complement(4465..5337)
                     /gene="ispE"
                     /locus_tag="PKK95_04560"
     CDS             complement(4465..5337)
                     /gene="ispE"
                     /locus_tag="PKK95_04560"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOC17515.1"
                     /translation="MMRAHAKINLTLLVGGRQPDGYHELHTIFQSLALHDTLDVHERP
                     GPFAIECDDPAVPTDSRNLVWRAAALVWEASGRPGPPSGALVTLCKRIPAQGGLGGGS
                     SNAAAALVAFERLWHPAMHPEMRLGLARRLGADVPFFLYGGTAAGAGRGDELTPLPDL
                     PPHHVVLVFPPFGVPTPDAYRWLDEDRGRDERHEALPAQEVHLADGTPIAIANDLEAP
                     VVARHPEIGRIRDALRAAGADGAALCGSGSTVFGLFRDAGAAAAAGAALAAAGWRTSI
                     TATTGREEAAGFLV"
     gene            complement(5334..6113)
                     /locus_tag="PKK95_04565"
     CDS             complement(5334..6113)
                     /locus_tag="PKK95_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17516.1"
                     /translation="MALLAGLLILVSAACGPRRLSLPSGAGQPFPGFGAAFDEATRPC
                     RAIDTMTAELGVSGRVGGQRLRGRVLAGFSRPASLRLEGLAPFGPPAFILVSSGGRAT
                     LLLPRDRAVLEGEPPEAIIDALVGLPLAPATLHAILAGCGLVASDATAGLAFGEGWAR
                     IEGAAGESTWLRQDGSGHWSVIAAVANSLRVEYEEREGGVPRRVLLELVDAEPRTELR
                     LRVAGADLNVALGDEAFTVEIPDKARPITLGELRRAGPLGR"
     gene            complement(6167..6985)
                     /locus_tag="PKK95_04570"
     CDS             complement(6167..6985)
                     /locus_tag="PKK95_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC17517.1"
                     /translation="MRWYGWTVSGMLVLLALSAAPAAASPQADRAQAYYRFLLARHLE
                     AQGEADRAATELREAAKLDPGAAEIRAELAALYARQDQPREAAQWGSAALEIDPSNRE
                     AHRVLGFVAAGQVGLDTSVRLTDAGGLAAAVRAIDHLEAARRTDRLADLAIELTLARL
                     YLRTGVPGKAVPVLQRVLLSDPDRPTVVLMLAEAYEASGQLEEAAAGFRRATQQRPRD
                     SDLHQRLGDVLFRLGRYREAVSAWQRALSGDLPPAEREGIERKIRTARELEPER"
     gene            7064..7237
                     /locus_tag="PKK95_04575"
     CDS             7064..7237
                     /locus_tag="PKK95_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOC17518.1"
                     /translation="MPIDPELLEILVCPACHGKVEVVRDGRGLKCQQCRRVYPVTEED
                     IPVMLVDEATIED"
     gene            7251..8357
                     /locus_tag="PKK95_04580"
     CDS             7251..8357
                     /locus_tag="PKK95_04580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC17519.1"
                     /translation="MRILLIRLRLIGDVVFTTPIIRALRRRHPGAHLAYVAEPAAAPI
                     VADNPHLDEVIVARAPGAAGRALADLSLARQLRRGRFDLAIDLHGGPRAATLALLSGA
                     RRRIGYAIPGRSWLYTDRVPRPRQLRARHSVENQWDLLGPLGVAPPDRERDATEMPVS
                     PAAAATAAAKLDAAGLADRGGLVVIHVSAGNPFRRWPAEAFAALAEGLVARDPRTRVL
                     LLSGPSEYEAARAIGARARAATGVSAARIVDQVELDLRELRAVLEDAALFVGGDSGPL
                     HVAGTSRVPVVGLYGPTLPARSAPWRPARYVTESVEVALPCRPCDQRRCEPGDFRCLG
                     RVTPAAVLAAAERAMAASGAQRTAKEGTWPLKPA"
     gene            8336..9526
                     /locus_tag="PKK95_04585"
     CDS             8336..9526
                     /locus_tag="PKK95_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC17520.1"
                     /translation="MAVEAGVKGTGHWLDRAALAGLLGFAGALQFSIFLAEALLAAAL
                     VAWLGALIVNRERPEVPGWFLPLLVYAALTLVATAFSLEPLTSLGAAKQLLLFLLVPA
                     TIRAARGRSAETVMDVVLSLGAFSAIVGVIQFGFLGYDRLQQRPVGTLGHWMTYSGLL
                     MLIACSAAARLLFERRNRTWPALIMPALVVALVFTFTRSAWVGACVGIGLLLVLKDFR
                     LLALAPVVAGLFFALAPASIASRFYSIFDLHDPSNRDRVAMFAAGAEIVASDPLTGVG
                     PNLMQRVYPDYRKPNAVEQTPPHLHNVPLQIAAERGLPALAVWIWFLVAVVRDLLRKL
                     RADRPRYLAAAALSAVGAMVAAGFFEYNFGDSEFLMLLLLLLALPWAASASPARRPQG
                     EPAA"
     gene            9543..10538
                     /gene="rfaE1"
                     /locus_tag="PKK95_04590"
     CDS             9543..10538
                     /gene="rfaE1"
                     /locus_tag="PKK95_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOC17521.1"
                     /translation="MALNESLQRALERFAGATVLIVGDVMLDQFVIGRVSRISPEAPV
                     PVVVYERDEHRVGGAANVANNVKALGGRVELVGLTGADAAGQRLRDLLNGLGMGTAGL
                     VRDPSRRTTTKLRVVTDRNQQVARIDYETDVEAAGPIEQALAIQAEQLVPRADVVVVS
                     DYLKGAVTRTLMARVIAAGRVRAIPVLVDPKIPHLDYYAGASLITPNHYEAEIATHLR
                     IRDDEDAVRASAVFRERAHCERVLITRGEHGMCLLDGDLPRHFPAVAREVADVTGAGD
                     TVIATMALGLAAGARIDQACELANHAAGIVVGRFGPAAVTRAELGEAVLGLGARG"
     gene            complement(10559..>10925)
                     /gene="purH"
                     /locus_tag="PKK95_04595"
     CDS             complement(10559..>10925)
                     /gene="purH"
                     /locus_tag="PKK95_04595"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOC17522.1"
                     /translation="PSREEWQALRFAWRVCAHVKSNAVIFTAADRTLAIGAGQMSRVD
                     AVKVATMKAGAARVDLAGSVAASDAYFPFRDGLDAVADAGATAVVQPGGSVRDAEVIA
                     AADERGLAMVFAGRRHFRH"
     assembly_gap    10926..11025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11026..12048)
                     /gene="purH"
                     /locus_tag="PKK95_04600"
     CDS             complement(<11026..12048)
                     /gene="purH"
                     /locus_tag="PKK95_04600"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOC17523.1"
                     /translation="MRRALISVSDKTGLESFARGLAELNFELVSTGGTARALAAAGLR
                     VTMVSEVTRFPEMLDGRVKTLHPHIHAGILARRSREDDRAALDRHGIHPIDLVAVNLY
                     PFARAAADPSTSFDALVEEIDIGGPSLLRAAAKNFRDVLVVTSPSDYGTVLEAIERSG
                     GPDLSLRLRLARAAFAHTAAYDAAIADELLLIEDGPGGYARTPRGVLPPALSLRLPKA
                     RDLRYGENPHLPAAWYRAPSAAGLDVRQGKELSFTNLLDIDAAARIVHEFREPAAVMI
                     KHTNPCGAATGATPAEAYVRAREADPLSAYGGISGLNRAIDEPTAREIASTFIEAVIA
                     PGIEPAA"
     gene            12301..12507
                     /locus_tag="PKK95_04605"
     CDS             12301..12507
                     /locus_tag="PKK95_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOC17524.1"
                     /translation="MRITGKVKWFNNAKGYGFIERDGGSDVFVHYSAIQSNGGFRSLE
                     EGQTVEFEIVDGPKGPQAGNVTKV"
     gene            complement(12563..13270)
                     /locus_tag="PKK95_04610"
     CDS             complement(12563..13270)
                     /locus_tag="PKK95_04610"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HOC17525.1"
                     /translation="MIVAEQSELERDLRLLEAALHQLESQYNMYFAGSLPKPPVEARS
                     RVEALFKRYDRAYIQSYADRFRLSTLQTRFSRFCELWDRGIRAREEGRPGPFQHMKRD
                     EFVDKGRPVTAPAEAPAPEPRAEPSPAPVEHAVRVGVAGDEALDSKIEGLYSALLEAR
                     RAAGNDQGLPFDRFADLVHGQLARLRTGGGREVVFRVATEEGKVVFTAKVSGSGSGAA
                     AAAREALNQGAVRSGKE"
     gene            complement(13289..13771)
                     /locus_tag="PKK95_04615"
     CDS             complement(13289..13771)
                     /locus_tag="PKK95_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17526.1"
                     /translation="MSEQRDHPRAGGPEGLARETAEALPPEHRRYRPRERFWPYVEVP
                     EEPTDEEIAALDPDLREELFGPSGRAFSVTIAFPRFDREDYAAAVAAARRASEYVETG
                     SGESFRHRARFLPRDVERLRDLWNVVGNLDQVDVLVDDRPVPYARELWLPLFWFLLLR
                     "
     gene            13831..>14708
                     /gene="selA"
                     /locus_tag="PKK95_04620"
     CDS             13831..>14708
                     /gene="selA"
                     /locus_tag="PKK95_04620"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOC17527.1"
                     /translation="MADFRLIPSIDTLRHRPRIKLLERHFGGPAIVDALRRAAAAVRG
                     DLARQAPGGPRGTGPGKEEIAEAIEARTEVELAASVRPSLGRVINATGVLIHTNLGRS
                     PLSPAALDRVREVAGGYSNLEYDLGTGGRGARDVHAEALLRRLLGVESAVVVNNNAAA
                     ALLVLAALAAGREVIVSRGELVEIGGGFRVPDVMAQSGAQLREVGTTNKTRVDDYRAA
                     ITDRTALILRVHRSNFRIEGFTEQPSLASLAALGRERGIPVAEDLGSGYIGMPGEEGD
                     EALDARLGGEPRVQASL"
BASE COUNT         2035 a         5345 c         5125 g         2103 t
ORIGIN      
        1 gacctatccc ggccatcacc ccgagcgtcg aaccgagggc caccagcagc tcggcgctcc
       61 agaacagctc ggccgccagc gccggcagca tcagcacggc cgcccaccgc ggcccgaacc
      121 gcgactcgaa cggatcgata agggtggtga actcgagcgc ccgcatccgg cgggcgaaga
      181 agacgccgcc caggacgagg ctgatgccga agcagagacc gccctgcacg ccgagcgcca
      241 cgctcgactt gaaggtgccc tccaccgtgc ccagcaggta cccgccgtcc acccacgtcg
      301 ccgtcatcgt gagcgtggcg agccagatcg gcatcgcgcg gcccgccacc agcagctcgg
      361 aaacgctgcc cccgcgagcg cggcgggccg cccgcaggcc gagtgcgagg aagacggcgt
      421 aaaggccgag gacggcaagg acggccctgt cggaggacgg gaagagaaga cgtggggtgg
      481 ccggtggctg gcggccggcc ggccgtcaca ggtggacggc cggcaaccag ccacgcgacg
      541 gtacgccgga cgggcgagac gcccgtccta ctggggctct gacggctcgg cggccggggc
      601 cggcgcgggc gcggcctgtc cctcgagcgg gacgaccttc accttcaccg tcgcggtcac
      661 ctcgcggtgg atcttcaccg gcacgtcgaa ctctccgagc tgcttcaagg gctcggccag
      721 caccacgcgc cgcttctcca cctcgaactg cttcccggcc agcgcctcgg cgatgtcggc
      781 gccggtgacc gacccgtaca gcgtctcgtt ctcgccgacg cggcgggcga acaccagctc
      841 gacctcgccc atccgggcgg ccaccgcctc ggcgtcctgc ttctcctggg cctcgcggac
      901 ctcggcaatc ttgcgctcgc gctcgatctg cttccggttc gcctcggtga ccggcaacgc
      961 cagcttccgt ggcaggaggt agttgcgggc gtacccgttc gccaccttca ccacgtcgcc
     1021 gcgccggccg aggttgtcga cgtgctcacg aaggatcact tccatggtcg attccccctc
     1081 agtcggtcac gtacggcacc agcgccagct gccgcgcccg cttgatggcc gtctgcagcg
     1141 cgcgctggtg cggcgcgcac acacccgaga tccgccgcgg gaggatcttg ccgcgatcgg
     1201 gcacgaacgg catcaacagc cggacgtcct tgtagtcgat gtagtcgatc ttctccgcgc
     1261 agaacttgca gaccttgcgc cggcggaaca gcgaccgccg ctgcccgggc cccttgtcgc
     1321 cgccgcgccc gccgcggccg ccccggccgc cgcccccgcc ccgtcgttcg tccgccatca
     1381 cagcacctcc gcatcttcac gaccgtcggt ccccgccgcg ggcgcctcgg cgccctcgcc
     1441 gccgccctgg ggcggccgcg ccaccgcctt ccgtcgcgcg cgtgcccgtt cggccgcccg
     1501 cagctcctcg tccacgcgca cgaccaggta gcgcatgatg cggtcgctca cgcggagccg
     1561 gcgctcgatc tccttcatca gctcgcccga gccgttgatc agctcgagca cgtagatgcc
     1621 ttccttgtgg gacccgatct cgtaggccat cttgcggcgg ccccagttct cggtcttcac
     1681 cagctcgccc gcgaaccggg ccaccgaggc ctcgacctgc gcgtgcaggt cggcgacctc
     1741 ctgctcggtc gcgtcgggcg acacgatgta gatcaactcg tactgcctgg aagtgctcat
     1801 gactctcctt ctggacgttt gctgaaacgg ccaccccagg gtggcaagga gccgactggg
     1861 ccgtgggact ggtgtctctc gtgttcctgt cagcgctccg gtgtcgcgcc cggggcatct
     1921 tcccgggcca gcgggtcggc gttgaagcgg ttcatcaccg cctcgatccc ctcgaggacg
     1981 aaaacctcgg ccgcgtcggc cgcacggcca atcgcctccg cgatctgcgg ccgctcctcg
     2041 ggatcgaagc cgcccagcac gtgatcggcg aggtcgcggc gcgcgtcgcc gcgccccacg
     2101 ccgacgcgca gccgggcgaa ctccccgttc cccagctgct cgatgatcga tcggaacccc
     2161 ttgtggccgc cgtccgatcc gcgcggccgc gcgcgcagcc gcccgagcgg cagcgccacg
     2221 tcgtcggcga cgaccagcag gtcgccgggc gcgacgcgga agtagcgcga cagctcggag
     2281 acggcgaacc cgctcaggtt catcatcgtg agcggcttcg ccaggatggc gccgtcaggt
     2341 cccaggcgcc gcgcccgcgc catcagcgcg tcggccggtg ccccctcgaa gcccgcgtcg
     2401 tgccggcgcg ccagctcgtc caccacagcg aacccgacgt tgtgccgggt gtcgcggtag
     2461 cggcggccgg ggttgccgag gccgacgatg agcttcagcg gcatgcagaa ggacccgccg
     2521 ctacttctcc gcctcccctt ccttctccgc cttgcccttc ttgatcacct cgggctcggc
     2581 gggcgcggcg gcggcttcgg tctcggcggc tgccggctcg gcctcggcct tctgcgccac
     2641 gacgtggacc agcatgaggt cgaggtcgct gaccggcttc cacttgacgc tctcgaccac
     2701 gtcccgcagc cggaggctct ggttgagcat caggcccgag atgtccacct cgatcgcctc
     2761 cgggatgtcg cccggcaggc actcgacctc cacctcgcgg tgcacgaagt cgagcaggcc
     2821 gccctgctgc ttgacgcccg gcgcctcgcc cttcagcgtg acggggacgg tgacggtgat
     2881 cgccttgtcc atcgcaacgc ggtagaagtc ggcgtgcagc agctggtgcg tcaccgggtc
     2941 gagctggtat tccttcacca tcacgcgcgg cgactcgccc tccagccgca gggtgatcag
     3001 ggtattggcg ccggtctccg agtggagaat ccgccagagg gccttgggct cgaccgcgat
     3061 cggcaacgcg tcgcgctggg cggttccgcc gtacagcacg ccgggcaggt gcccctctcg
     3121 ccgcaggcgg cccgcctcgt tcttgccgaa ccggtcccgc ttcaccgctt ccagtacagc
     3181 ttccatgttc tgtaacccgt ccttctcccc gctggtcgcc atggcaggcc gtgaaacgcg
     3241 cggcccgaag gcaagaggcg gcacactacc gagcagcccg aggtcgagtg gcaccacagg
     3301 ctgctagacg aacaacgacg acaccgagct ctcctcgtgg atgctccgga tggcctgcgc
     3361 gagcagccgg gccaccgaca ggacgacgat cttcgggcac cggctcttct cgggcccgag
     3421 cggcgtcgtg ttggtgacga tcagcttctc gatcggcgac tgctcgatcc gcccgattgc
     3481 cggccccgac agcaccccgt ggaccgcgca ggccagcacc cgctcggccc cgttctccac
     3541 cagcgccgtc gccgccttct gcatcgtccc ggcggtgtcg acgatgtcgt cctgcaggat
     3601 gcaggtgcgg ccggcgacgt cgccgatgac gttcatcacc tcggcggccc cggtctcgga
     3661 ccgccgcttg tcgatcaccg cgagccccgc ccccagccgc ttcgcgtacg cccgtgcgcg
     3721 ctcggccccg ccggcgtcgg gcgagacgat cgtcatgttc gggaaccgct ggcgcgccag
     3781 gtagtcgatg atcaccggcg ccgcgaacag gtggtccacc gggatgtcga agaagccctg
     3841 gatctgcgcc ttgtgcaggt ccatcgtcag cacacggttc gccccggcgg cgctcaggat
     3901 gttggccacc agctttgccg agatcggtac ccgcggcttg tccttccgat cctgccgcgc
     3961 gtagccgtaa tacgggatca ccgctgtgat ccgggccgcc gacgaccgcc ggaaggcgtc
     4021 gatcatgatc agcagctcga tcaggtgctg atcgaccggg gtgctcgtcg gctgcaccac
     4081 gaagacgtcg gtcccccgga tgttctcctc gatctgaaac gacacctcgc tgtcggggaa
     4141 ccgtccgatc cgggcctggc cgaggggcag gcccagatgg ccggcaatct cgcgcgccag
     4201 ctcggggtgc gcgctgccgg agaacacctt cagttcggcg aaagccatcc tcatggcctc
     4261 accctcgtgc cggacatcga aacggccctc ccggggggct cctccagtgc tggctggggc
     4321 ggaaggattc gaaccttcga tgcgggatcc aaagtcccga gccttaccac ttggccacgc
     4381 cccaacacgt cgcggccgcg cagccaacca tggcgcgacc cgcaggcgcg gcgcaaacag
     4441 ttagtgtata cgacagcttg cgcttcagac aaggaagcct gccgcctcct cccgcccggt
     4501 cgtcgcggtg atcgacgtcc gccagcccgc ggcggccagc gccgccccgg ctgccgcggc
     4561 ggcccctgcg tcgcggaaca gaccgaatac cgtcgagccg ctcccgcaga gggccgcgcc
     4621 gtccgccccc gccgcacgaa gggcgtcccg aatccgaccg atctccgggt gcctcgcgac
     4681 aaccggcgcc tcgaggtcgt ttgcaatcgc gatcggcgtc ccgtcagcca ggtgaacctc
     4741 ctgcgcgggc aaggcttcgt ggcgctcgtc tcttcctctg tcctcgtcca gccaccggta
     4801 cgcgtcgggg gtcggcaccc cgaacggcgg gaagacgagc acgacgtggt ggggcggcag
     4861 gtcgggcagc ggcgtcagct cgtcgccgcg cccggctccg gccgcagtcc caccgtagag
     4921 gaagaagggc acgtcggcgc ccaggcggcg ggccagtccg aggcgcatct ctgggtgcat
     4981 tgccggatgc cagaggcgtt cgaacgcgac cagcgccgcg gccgcgttcg aactgccgcc
     5041 tcccagtccc ccttgggcag ggatccgctt gcacagcgtg acgagggcgc ccgagggcgg
     5101 gccggggcgg cccgaagcct cccaaacgag ggcggccgcg cgccagacca ggttccggga
     5161 gtcggtcggc accgcggggt cgtcgcactc gatggcgaac ggacccggcc tctcgtgaac
     5221 gtcgagcgtg tcgtgcaacg ccagcgactg gaagatggtg tgcagctcgt ggtagccgtc
     5281 gggctggcgg ccgcccacga gaagcgtcag gttgatcttg gcgtgcgctc tcatcatcga
     5341 ccgagcgggc cggcccgccg cagttcgccg agcgtgatcg gcctcgcctt gtccgggatc
     5401 tcgacggtga acgcctcgtc accgagcgcg acgttcaggt cggcgccggc cactcgcagc
     5461 cgcagttcgg tgcgcggctc ggcgtccacc agttcgagca gcacacggcg cggcaccccg
     5521 ccctcccgct cctcgtactc gacgcgcagc gagttggcca cggccgcgat caccgaccag
     5581 tgacccgacc cgtcctgccg cagccacgtc gattcacccg ccgccccctc gatgcgcgcc
     5641 cacccctcgc cgaaggcgag tcctgcggtg gcatcggacg cgacgaggcc gcagccggcg
     5701 aggatcgcgt gcagcgtggc cggggcgagc ggaaggccca cgagcgcgtc gatgatggct
     5761 tccggcggtt ctccctcgag gaccgcccgg tcgcgcggca gcagcagcgt cgcccgtcct
     5821 cccgacgaaa cgaggatgaa ggctggcggc ccgaacgggg caaggccctc gaggcgaagg
     5881 ctggccggcc gcgagaaccc cgcgaggaca cggccccgca gccgctggcc gccgacgcgc
     5941 cccgagacgc cgagttccgc ggtcatcgtg tcgatggccc ggcacgggcg ggtcgcctcg
     6001 tcgaaggccg ccccaaaacc ggggaacggc tgcccggcgc ccgatggcag cgagagccgc
     6061 cgcggaccgc aggcggcaga gaccaggatc agcaagccgg cgaggagtgc caacgcgggg
     6121 cggttccgcc gcccaggccc ttgcggcagg ccccccggcc aagccctcac cgctccggct
     6181 ccagctcgcg ggccgtgcgg atcttgcgct cgatgccctc gcgctcggcg ggcggaaggt
     6241 cgcccgaaag cgcgcgctgc caggcggaga ccgcctcgcg gtaccgaccg aggcggaaca
     6301 ggacgtcgcc aaggcgctgg tgcaggtccg agtcgcgggg gcgctgctgg gtggcgcggc
     6361 ggaagccggc cgcagcttcc tcgagctgcc cgctggcctc gtaggcctcc gccagcatca
     6421 gcacgacggt cggacggtcg gggtcggaca ggagcacgcg ctgcagcacc ggcacggcct
     6481 tgccgggcac gccggtgcgc aggtagagac gggccagggt cagctcgatc gcgagatcgg
     6541 ccaggcggtc ggtccggcgg gccgcctcga ggtggtcgat ggcgcgcacg gccgcggcaa
     6601 ggccgccggc gtcggtcagc cggaccgagg tgtcgaggcc aacctgcccc gcggcgacga
     6661 acccaagcac gcggtgggcc tcgcggttgg acggatcgat ttcgagcgcc gcgcttcccc
     6721 actgcgcggc ctcacgaggc tggtcctgcc gcgcgtaaag cgccgccagc tcggcgcgga
     6781 tctccgcggc accggggtcg agcttcgccg cctcgcgcaa ttcagtggcc gcccgatcgg
     6841 cctcgccctg agcctcgagg tgccgggcaa gcaggaagcg gtagtaggct tgagcgcggt
     6901 cggcctgggg cgaggcggcg gccggcgcag cagagagcgc cagcagcacg agcatgcccg
     6961 aaacggtcca cccgtaccat ctcacgcccc gattgttcca caaaagccgc cgcaggagat
     7021 agccgcgcgg ccggactgcg ctatagttcc gacggaggcg ccgatgccaa tcgaccccga
     7081 gctgctcgag atcctggtgt gccccgcctg tcacgggaag gtggaggtgg tgcgtgacgg
     7141 tcgcgggttg aagtgccagc agtgccggcg ggtgtacccg gtcaccgagg aggacatccc
     7201 ggtcatgctg gtggacgagg cgacgatcga ggactgatga accgacggcc gtgaggattc
     7261 tcctgattcg cctgcggctg attggcgatg tcgtgttcac gacgccaatc atccgggcgc
     7321 tgcgccggcg tcaccccggg gcccatctcg cctacgtggc ggaaccggct gccgccccga
     7381 tcgtcgcgga taacccccat ctcgacgagg tcatcgtggc gcgggcgccg ggcgcggccg
     7441 ggcgcgcgct ggccgatctc tcgctcgccc ggcagctcag gcgcggccgt ttcgacctgg
     7501 cgatcgatct ccacggaggg ccccgtgccg cgacgctcgc gctgctcagc ggcgccagac
     7561 ggcggatcgg gtacgcgatc cccggccgga gctggttgta cactgaccgc gtgcctcggc
     7621 cgcggcagct gcgcgcgcgg cactcggtgg aaaaccagtg ggatctcctg gggccgctcg
     7681 gcgtcgcccc ccccgatcgc gagcgcgacg ccaccgagat gccggtctcg ccggcagccg
     7741 ctgcgaccgc ggcggccaag ctcgacgcgg caggcctcgc tgatcgcggt ggcctcgtgg
     7801 tgatccacgt gagcgccggg aacccgtttc ggcgctggcc ggccgaggcg ttcgcggcgc
     7861 tggccgaggg gctggtcgcg cgcgacccgc gcacgcgggt gctgctgctc tccggcccat
     7921 ccgagtacga ggccgcccgg gccatcggcg cgcgggcccg cgccgccacg ggcgtgtcgg
     7981 cggcgcggat cgtcgaccag gtcgagctgg acctcaggga actgcgcgcg gtgctcgagg
     8041 atgcggcgct attcgtcggc ggcgacagcg ggccgctcca cgtggccggc acctcgcggg
     8101 tcccggtggt cggcctctac gggccgacgc tgcccgcgcg gtcggcgcca tggcgcccgg
     8161 cgcgctacgt caccgagtcg gtggaggtcg ccctgccgtg ccggccgtgc gatcagcgcc
     8221 ggtgcgagcc gggagatttc cgctgcctcg ggcgcgtcac gcccgcggcc gtgctggccg
     8281 ccgccgagcg ggcgatggct gcgagcggcg cgcagcgaac agcaaaggaa gggacatggc
     8341 cgttgaagcc ggcgtgaaag gcaccgggca ctggcttgac cgtgcggcgc tggcgggcct
     8401 gctcggcttc gccggggcgc tgcagttctc gatcttcctg gccgaggcgc tgctggcggc
     8461 ggcgctggtc gcctggctcg gcgccctgat cgtgaaccgc gagcgtcccg aggtccccgg
     8521 ctggttcctg ccgctgctcg tgtacgccgc gctgacgctg gtcgccaccg cgttttcgct
     8581 cgagccgctg accagcctcg gggccgccaa gcagctgctg ctgttcctcc tcgtcccggc
     8641 caccattcgg gcggcgcgcg ggcgctcggc cgagacggtg atggacgtgg tgctgtcgct
     8701 cggggcgttc agcgcgatcg tgggggtgat ccagttcggc ttcctcggct acgaccggct
     8761 gcagcagcgg ccggtgggaa cgctcgggca ctggatgacg tactcggggc tgctgatgct
     8821 gatcgcctgc agcgcggccg cgcggctgct gttcgagcgg cgcaaccgca cctggccggc
     8881 gctcatcatg cccgcgctcg tcgtggccct cgtgttcacc ttcacccgga gcgcgtgggt
     8941 cggcgcgtgc gtgggaatcg gcctgctgct cgtgctgaag gacttccggc tgctggcgct
     9001 cgcgcccgtg gtggccggcc tcttcttcgc gctggcgccc gcgagcatcg cgtcgcgctt
     9061 ctactccatc ttcgacctcc acgacccgag caaccgcgac cgggtggcga tgttcgcggc
     9121 gggggccgag atcgtcgcga gcgacccgct gaccggcgtc gggccgaacc tgatgcagcg
     9181 ggtctacccg gattaccgga agccgaacgc ggtcgagcag acgcccccgc atctccacaa
     9241 cgtgcccctc cagatcgcgg ccgagcgcgg tctgcccgcc ctcgccgtct ggatctggtt
     9301 cctcgtcgcc gtggtgcgcg acctgctgcg caagctgcgg gccgaccgcc cgcgctacct
     9361 ggccgccgcg gcgctcagcg cggtcggcgc gatggtggct gccgggttct tcgagtacaa
     9421 cttcggcgac tcggagttcc tgatgctcct cctcctgctg ttggcgctgc cctgggcagc
     9481 ctcggcgtcg ccggcccggc ggccgcaggg ggagcccgcc gcctgacccg ggggtcttcg
     9541 acatggcgct caacgaatcg ctccaacgcg cgctcgaacg cttcgccggc gccacggtgc
     9601 tcatcgtcgg cgacgtgatg ctcgaccagt tcgtcatcgg ccgcgtgtcg cgaatctcgc
     9661 ccgaggcgcc cgtgcccgtc gtcgtgtacg agcgcgacga gcaccgggtg ggtggcgcgg
     9721 cgaacgtcgc caacaacgtc aaggcgctcg ggggccgggt cgagctggtc gggttgacgg
     9781 gcgccgacgc ggccgggcag cggctgcgcg acctgctgaa cggcctcggc atgggcacgg
     9841 cgggcctcgt tcgcgacccc tcgaggcgaa ccaccacgaa gcttcgggtg gtgacggacc
     9901 gcaaccagca ggtcgcgcgg atcgattacg agaccgacgt cgaggcggcc ggccccatcg
     9961 agcaggcgct cgcgatccag gccgagcagc tcgtcccgcg tgccgacgtg gtggtggtct
    10021 ccgactacct gaagggggcg gtgacgcgaa cgctgatggc gagggtgatt gccgccggga
    10081 gggtgcgcgc gatcccggtg ctcgtcgatc cgaagatccc gcacctcgac tactacgccg
    10141 gcgcctcgtt gatcacgccc aaccactacg aggccgagat cgccacgcac ctgcggattc
    10201 gcgacgacga ggacgcggtg cgcgcctcgg cagtgttccg cgagcgcgcg cactgcgagc
    10261 gggtgctgat cacgcgcggc gagcacggga tgtgcctgct cgacggcgac cttccccggc
    10321 acttccccgc cgtcgcccgc gaggtggccg acgtcaccgg cgccggcgac acggtgatcg
    10381 ccacgatggc cctcggcctg gccgccggcg cgcggatcga ccaggcgtgc gagctggcca
    10441 accacgcggc cggcatcgtg gtcgggcgct tcggtcccgc cgccgtcacc cgcgcggagc
    10501 tgggggaagc ggttctgggg ctaggggcta ggggctaggg ggccgagtcg ccggcctgtc
    10561 agtgcctgaa gtgcctccgg ccggcgaaca ccatcgcgag gccgcgctcg tcggcggcgg
    10621 cgatcacctc ggcgtcacgc actgacccgc cgggctggac gaccgcagtc gcccccgcgt
    10681 ccgccaccgc atcgagaccg tccctgaagg ggaagtacgc gtccgacgcg gccaccgacc
    10741 ccgcaagatc cacgcgcgcc gcgccggcct tcatcgtcgc caccttcacc gcgtccacgc
    10801 ggctcatctg ccccgccccg atggccagcg tcctgtccgc agccgtgaag atcaccgcgt
    10861 tcgacttgac gtgagcgcag acgcgccacg cgaagcgcag cgcctgccac tcctcgcggc
    10921 tcgggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggcgg ccggctcgat
    11041 gccgggcgcg atcaccgcct cgatgaaagt gctcgcaatc tcgcgggcgg tcggttcgtc
    11101 gatcgcccgg ttgagccccg agatcccgcc gtaggcagac agggggtcgg cctcgcgggc
    11161 gcgaacgtac gcctcggcgg gcgtggcgcc ggtggccgct ccgcaggggt tggtgtgttt
    11221 gatcatgacg gcggccggtt cgcggaactc gtggacgatg cgggcggccg cgtcgatgtc
    11281 gagcaggttg gtgaaagaga gttccttccc ctggcgcacg tcgagcccgg ccgcgctcgg
    11341 cgcccggtac caggccgcgg gcaggtgcgg gttctcgccg tagcgcaggt cgcgcgcctt
    11401 cggaagccgc aggctgagag cgggtggcag gacgccccgc ggcgtccgcg cgtagccgcc
    11461 cggcccgtcc tcgatcagca gcagctcgtc agcgatggcg gcgtcataag cggccgtgtg
    11521 ggcgaaggcc gcacgcgcga gccgcagccg cagcgacagg tcgggccccc cgctccgctc
    11581 gatcgcctcg agcaccgtgc cgtagtcgga cggcgaggtc acgacgagca cgtcgcggaa
    11641 gttcttcgcc gcggcgcgca acaggctcgg ccccccaatg tcgatctcct cgacgagcgc
    11701 gtcgaacgag gtcgagggat ccgccgcagc gcgggcgaac gggtaaaggt tcaccgccac
    11761 gaggtcgatg ggatggatgc cgtggcggtc gagcgcggcc cggtcgtcct cacggctgcg
    11821 ccgggccagg atgccggcat ggatgtgggg gtgcagcgtc ttgacgcggc cgtcgagcat
    11881 ctcgggaaag cgggtgacct cggacaccat cgtcacgcgc aggccggcgg ccgccagcgc
    11941 ccgcgcggtg cctcccgttg ataccagttc gaagttcaac tcggcgaggc cgcgtgcaaa
    12001 cgactccaaa cccgtcttgt ccgatacact gatcaacgct cgacgcatgc gccaccctcg
    12061 acggggatga accccgcaaa actcaccata tttcgaagct tttgcgattg tggggcgccg
    12121 ttgtccgctt gacactcatc tgccgccccc ataagatgga gggtggcgcc gatcgttcgg
    12181 gcctccggcg ccgagccgcg tacggtgcgg cttttttttg ttcccccacg gtccgcgaaa
    12241 cgactcgggg gcagtctcgt cggtcggcgc ggcgcgccag accaagcaag gcaggaaaga
    12301 atgcgcatta caggcaaggt caagtggttc aacaacgcga agggttacgg gttcatcgag
    12361 cgggacggcg gcagcgatgt cttcgtccac tactccgcaa tccagagcaa tggcggtttc
    12421 cgctccctcg aggaaggtca gaccgtcgag ttcgagatcg ttgacggccc gaagggcccg
    12481 caggctggca acgtcaccaa ggtctagcgc cgagcgacgg ggcgtccagc gcgtgcgccg
    12541 ggcgcccccc cccgcccatc cctcactcct tcccactcct caccgctccc tgattcaaag
    12601 cctccctcgc agcggcagcc gcgcccgacc ccgaccctga gaccttcgcg gtgaagacca
    12661 ccttgccctc ttcggtggcg acgcggaaga ccacctcgcg gcccccgccc gtgcggaggc
    12721 gcgccaactg cccgtgaacg aggtcggcga accggtcgaa cggcagcccc tggtcgttcc
    12781 cggccgctcg gcgggcctcg aggagggccg agtagaggcc ctcgatcttc gagtcgagcg
    12841 cctcgtctcc ggccacgcca acgcggacgg cgtgctcgac gggcgcaggg gacggctcgg
    12901 ctctcggctc gggcgccggc gcttcggccg gggcggtgac gggccgcccc ttgtccacga
    12961 actcgtcacg cttcatgtgc tggaagggcc ccggacgccc ctcctcgcgg gcacggatcc
    13021 cgcggtccca cagctcgcag aaccggctga acctggtctg gagcgtgctc aagcggaagc
    13081 gatcggcgta gctctggatg taggcgcggt cgtagcgctt gaagagtgcc tctacgcgcg
    13141 accgcgcttc gacgggcggc ttgggaagcg acccggcgaa gtacatgttg tactggcttt
    13201 ccagctgatg gagggctgct tccaggaggc gaagatcgcg ttcgagctcg ctctgttcgg
    13261 ccacgatcac ccctgcgatg gacgcgggct accggagcaa gaggaaccag aacaacggca
    13321 gccagagctc gcgcgcgtaa ggaaccgggc ggtcgtccac gagcacgtcc acctggtcga
    13381 ggtttccgac gacgttccac aggtcgcgca ggcgctcgac gtcgcgtggc aggaaccgcg
    13441 cgcggtgcct gaacgactcg cccgatcccg tctcgacgta ctcgctcgcg cggcgagccg
    13501 ccgcgacggc cgccgcgtag tcctctcggt cgaagcgggg aaacgcgatc gtcaccgaga
    13561 aggcccggcc gctcggcccg aacagctcct cgcggaggtc cggatcgagg gcggcgattt
    13621 cttcatcggt gggctcttcg ggcacctcga cgtacggcca gaaccgctcg cgggggcggt
    13681 agcggcgatg ttcggggggc agggcttcag ccgtctcgcg ggccaggccc tcggggccgc
    13741 cggctcgagg gtgatcacgc tgttcgctca tgtacagggc caagacaacg gtgatggtat
    13801 cctgactccg ctacgagcgg caagagagcc atggccgatt tccgcctgat cccgtcgatc
    13861 gacacgctgc gccaccggcc ccggatcaag ctgctcgagc gccacttcgg gggacccgcc
    13921 atcgtggacg ccctgaggcg ggcggccgcg gccgtgcgcg gcgacctggc gaggcaggcg
    13981 ccgggcggcc cccgcggcac cgggcccggg aaagaggaga tcgccgaggc gatcgaagcg
    14041 aggaccgagg tcgagttggc cgcgtcggtg cggccgtccc tcggccgggt gatcaacgct
    14101 accggcgtct tgatccacac gaacctcggg cgctcgccgc tctcgccggc cgccctcgat
    14161 cgcgtccgcg aggtggccgg cgggtactcc aacctcgagt acgatctcgg cacgggcggc
    14221 cgcggcgccc gggacgtcca tgccgaggcg ctgctccgcc gcctgctcgg cgtcgagtcg
    14281 gccgtcgtcg tcaacaacaa cgcggcggcg gcgctgctgg tgctggcggc gctcgccgcc
    14341 gggcgcgagg tgatcgtctc gcgcggcgaa ctggtcgaga tcgggggagg ctttcgggtt
    14401 cccgacgtca tggcccagtc gggagcacag cttcgcgaag tcggcaccac caacaagacc
    14461 cgcgtggacg actaccgggc ggcgatcacc gaccgcacgg cgctgatcct ccgcgtccac
    14521 cgctccaact tccgcatcga ggggttcacc gagcaaccgt cgctggcttc cctcgccgcg
    14581 ctcggccgcg agcgcgggat cccggtggcc gaggaccttg gcagcggcta catcggcatg
    14641 cccggggaag agggcgacga ggcgctcgac gcgcgccttg gaggcgagcc gcgggtgcag
    14701 gccagcct
//
LOCUS       DAOPQX010000052        14439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4906_length_14439_cov_4.500000, whole genome shotgun sequence.
ACCESSION   DAOPQX010000052 DAOPQX010000000
VERSION     DAOPQX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4906_length_14439_cov_4.500000"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKK95_04625"
     CDS             <1..589
                     /locus_tag="PKK95_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17528.1"
                     /translation="LGYPVFSQSTLPLDDDLLEELFGDEVRAGVVRDPLEIQDVWKNS
                     YSASTNHKVWAAKFTARHPNLVALELSNFKCGHDAPIYTVIESIIEKSGTPYFSFKDV
                     DESKPTGSIRLRVETIHYFLKRYAEDMNRKRDAGKAIDEQVAAFERQLRRQMLLDEEL
                     ADIAARQPEFPAGRRLLPVLPLGQGGSSPGRELHS"
     gene            728..2851
                     /locus_tag="PKK95_04630"
     CDS             728..2851
                     /locus_tag="PKK95_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOC17529.1"
                     /translation="MRSRTRIGSRLGAVAIVFLLAAFAEVRPPARAQSFDRYFPAKDL
                     TTVGVYYYPEHWDPAQWERDFRNMAAMGFEFTHFAEFAWAQLEPEEGRYDFRWLDRSV
                     ELAAEHKLGVVLCTSTATPPVWLVRKYPEVLATAEDGSRMDHGSRQHASFSSTFYREY
                     SLKLIEQLARRYGGDERVIGWQLDNEPRRFLDYGKDAPARFRAWLEKKYGTIDALNAA
                     WGTAFWSGTYTSFSQINLPLHRQWGMNLHQRLDHYRFADEETATFLDEQARVIRKHVK
                     GRQWITSNYIPMYDVGYVGASRELDFDSYTRYMVYGADRGLGPRGYRVGEYTRIAMAN
                     DFFRPLKGMYGVMELQPGQVNWGAINPQPLPGAVRLWLWHVFSGGSKFTCTYRYRAPL
                     YGYEAYHAGIVGTDGVTPTAGGREYAQFIADIRRLREKADAGAPVPAAYARRATGILL
                     DADSIQAIGLNRQTTEWNTESHVLKYYRALKSFGAPVDFVRRGADLARYPVLVVPAHQ
                     MIDEELIAALTRYAEQGGTLLLTCRTGLHDRRGHLWEAKFYEPMWKLVGAEIESYDLP
                     MPHAPNTVRFGSEDFEWSSWGDLLKPRPGTATWATHQGDFYAGTPAVVSRKLGKGTVT
                     WVGVDTRSGQLEKQVLARLFAERGIAVEGYPEGVLVEYRDGLGIALNYSDKDFVMPLP
                     AGTEILVGDTTLETASVLVWKTPRG"
     gene            complement(2976..3164)
                     /locus_tag="PKK95_04635"
     CDS             complement(2976..3164)
                     /locus_tag="PKK95_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17530.1"
                     /translation="MAEAGCATCKIREKADRKPTSLLARLWRWHAGWCPGFKSYMQAL
                     PDAERRALADRYNLAKYR"
     gene            complement(3322..4950)
                     /locus_tag="PKK95_04640"
     CDS             complement(3322..4950)
                     /locus_tag="PKK95_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOC17531.1"
                     /translation="MTCSARALAPALALLLTIALPAFGADRVKTANGIVESTVAPVEG
                     VRSFKGIPFAQPPVGDLRWREPQPPKNWTGVRNADGFGPACMQRLSPGADYWLRGDGM
                     SEDCLYLNVWTPAKSGREKLPVLVYIFGGGFQNGDGSEPRYDGERMARKGIVAVSLNY
                     RTNVFGFFAHPELTKESPHGASGNYGLLDQVAALRWVQKNIAAFGGDPKRVTIAGESA
                     GSISVSALMASPLSRGLIAGAICESGAAIASLPPRALREVEASGVKFAESAGAGSLAA
                     LRAMSAQQILEAAGKARDIRFSTAMDGYFLAKSLPEIALAGEQAKVPLLAGSNTEEGP
                     ARAVLAGGEPTAETLAAAIRKFYGEKAESIVKAYAATTPDEVYEAAMHLASARFVSYG
                     TWKWTELHMKTGGKPVYRYLYARVRPPYLGMPGQPPPAVPAAARGDAAGSAGPRPPRG
                     AAHSAEIQYAMGNLELDTRYAWEPADHEVSKTMQAYFVNFIKTGNPNGPGLPEWPAYR
                     PETNYQRMRIDVKSQAEPEAHRDRYLALDAAIGH"
     gene            5122..5694
                     /locus_tag="PKK95_04645"
     CDS             5122..5694
                     /locus_tag="PKK95_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOC17532.1"
                     /translation="MPPFDRPASYDDLLRQPDHLVAEILDGELHANLRPSAVAAGSAF
                     TLGGMLRRACSAGDPDGWQILNEPELRLGADVLFPAAAGWRASRLPELPRTAYFSIAP
                     DWVCEIPATYPASLRSRRMAIYAGEGVSYAWLIDPVAQTLEVRSLQEGRWRVVATHVG
                     GQLVRAVPFEALELQLSALWPREPGKTRGR"
     gene            complement(5705..8014)
                     /locus_tag="PKK95_04650"
     CDS             complement(5705..8014)
                     /locus_tag="PKK95_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOC17533.1"
                     /translation="MNEGSIEKRAGSGLPARIGRYAITSMLGQGGMGVVYAARDERLG
                     RSVALKTMASPARDEAARQRFWREARSAASVSHPNVCQIYEVGEDDGELFIAMELLEG
                     ESLAGRLRRGPMNVSEALPVALGILAALSELHARGIVHRDLKPSNVFLTRHGVKVLDF
                     GLARPEAGAVDASATGLTTIGSIIGTPHYMAPEQVRGETVDARCDLFAAGAILFEMLA
                     GRPAFDGASAVEVLHATLHDQPPALTGSPAVAAVDRVIRRALAKRPEDRPASAEAMAR
                     QLRGIRHVDGASAPTLARPLLRLVVLPFRVLRPDPETDFLAYSLPDAIATSLSGIDSL
                     VVRASATAARFADAPDPKALASEADVDRVVMGTLLRAGDQLRVSVQLVEAPGGTLLTS
                     HTVQSSMGDLFHLQDDISQRVFDALSLPLTAEAAPPEPDAPHDPEAYELYLRANEMVR
                     TYDGLPRARHLYELCLERDPGFAPAWAHLGRCQRVIGKFIEEQPDSEARAENALRQAL
                     AINPRLSIAHKFYAHLEADMGQPQQALRRLVEQAKLNGNDPELFAGLVHACRYCGLYD
                     ESIAAHAEARRLDPNVPTSVEQTLLMAGDVERLLEIPRPLVVAGADDGMRVIALGFAG
                     RREAARLAADEMRRIPRIPAFRAWSEALFAWVERRLDDVLAHLATLRGLKIQDDPEAL
                     FLEAWLLCDLGDHDEGARLIERAVAKGYCPSQLLRNRPEFEALRRRAAFQAVLAEADA
                     NRLRGREVFREAGGERLLGQPRSSSSSGS"
     gene            8215..9513
                     /locus_tag="PKK95_04655"
     CDS             8215..9513
                     /locus_tag="PKK95_04655"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC17534.1"
                     /translation="MSIFRTIAFFLTLIAASVSAGQQPAAPAPGSRQVVAPAGAFSAE
                     RLARLDRVLQQYVDEDRAAGIVALVLRDGKPVYDRAFGWSDKEAGHRMTTDTIFRIAS
                     QSKALTSVAVLSLMEEGALSLNTPVSAFIKSFAKTSVVVKKDGDVATVAARRPITIRD
                     LLTHTAGISYGTDATVAALYEPKGLGPAAGWGWYTADKDEPVCDTMERLGTLPFVAQP
                     GEAFVYGYNTDILGCVVERASGMPLDRFIASRITGPLGMKDTHFFLPPGQRARLAAVY
                     GNGPDGKIARAPEGSRGQGHYVDGPRKSFAGGAGLLSTAHDYARFLEMIRRGGELDGV
                     RILSPRTVALMTTNQVGDLYRTPGLGFGLGFETTERYGASGMASVGAFGWGGAYGTTY
                     KVDPQARLVLVMMVQLLPNTTDIGQKFPNLVYQALMEPVE"
     gene            9510..9893
                     /locus_tag="PKK95_04660"
     CDS             9510..9893
                     /locus_tag="PKK95_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17535.1"
                     /translation="MRTLASVAVVSLLLGLPAASRAAAPAPGVPANGGVKVPGPWVAL
                     HVLNYETDADLAALEQQLPALAARGVNVLVLEVDYAFESLALGMPGVLGHLFTIWSKQ
                     PNVADWPPLAANASLVRQLAGGKGR"
     gene            complement(9916..10878)
                     /locus_tag="PKK95_04665"
     CDS             complement(9916..10878)
                     /locus_tag="PKK95_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17536.1"
                     /translation="MKRVLILAGLLMCVLGEVASAAELRIGIIGLDTSHVIAFTRSLN
                     DASSPGHVPGAKVVAAFKGGSPDVPASATRVDKFTADLQSTYGVRIVGTIAELCDLVD
                     AVLLESVDGRVHLEQAREVLRRRKPLFIDKPMAASLADAKEIARLAQESGTPWFSSSS
                     LRFASAYQEFLADPARGQVLGVEAHGPASLEPTNPGLFWYGIHAVETLYTLMGPGCTS
                     VTMTSNADYDLAVGVWKDGRIGTVKGLRTGKQDYGALVYGDKAVTYLPVKDVSYVPLV
                     RQIVAFFQTGKPPVPPEETLEIMAFMDAAERSRVKGGVPTDLAR"
     gene            complement(10912..12252)
                     /locus_tag="PKK95_04670"
     CDS             complement(10912..12252)
                     /locus_tag="PKK95_04670"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17537.1"
                     /translation="MKRSRREFIRSVSGGAAAAAFVGLGPGFTARGRAQVAGANDRIH
                     VAVMGLNGRGHSLAGTFGKQPDCRVAWVCDVDRRAIEKTAAAVAGIQGNAPKTTNDFR
                     RCLEDPEVQALVVAAPDHWHAPATLLACKAGKDVYLEKPCSHSPAEGEMLVAAARKYG
                     RKVQLGTQRRSWPNVVEAVKELRGGIIGRPYFAKGWYTNNRKSIGFGKEAPVPDWLDY
                     ELWQGPAPRRPFRDNLVHYNWHWFWHWGTGEALNNGTHMLDLMRWGLGVEYPVRVGSS
                     GGRFHFVDDWEPPDTQVITLDYAEKVSIAWEGRSCSGRTIDGSGAGAIFHGENGSLLV
                     DGNGYLVQDLDEKVVREVKSATDIDPRNPTNPADSLDVVHVRNFLDSIRGTASLNADI
                     HEGHVSTLLCQLGNIAFRTGRSINIDRATGHVVGDPEAAKYWEREYEKGWEMVL"
     gene            complement(12357..>14439)
                     /locus_tag="PKK95_04675"
     CDS             complement(12357..>14439)
                     /locus_tag="PKK95_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOC17538.1"
                     /translation="VSQPLPAAAGARSAGASLEIPAPVPLRAHSASRRTSLRIDRFYY
                     ETARGRTRLPASLTSRVGTGGVGEAKMADGRTVRLEVSSDGNHRALRLTVEPSRGVRG
                     WGLAIDAGPDEYFTGLMERVVDGPQERSWLPGLEKAMNLRGQRVSMIMKRTTSLYAPF
                     YLSSRGYGVFVKGTTRGHFGFCVDARDRVTIAFEGTSLEMKLYLDPDPAVIVRDHAID
                     AGPPILPPKWTFTPWRWRDEHVHRDRYYDGTRVKGPFNADVMEDVLMMKAYGIPNGVY
                     WIDRPWGSGRHGYDDFEIDEKRLPNFEEMVRWLGRQGTRTMLWIGPFFQGQMARTALA
                     AGYHMPGHRWPNFNNYPLADFTNPEARAFWQEGFSKLLKLGVAAFKLDRADEDFPQEP
                     LHVFDGTSLREHRSAYPVVFLRAAYEEARKHRGDDFLLMPRAAYTGSAPYGVFWGGDV
                     FGTQEGLRASIIAVQRASVMGYPIWGSDTCGYNRQLLEQEVCARWLAFSAFTPIMEVG
                     PTRNTGFWNLPRKPRYDERLIATWRLYARLHLRLADYTYRHAREAAATGMPIVRPLFL
                     ADPSAHQAWTHWETYLYGADLVVSPIWKKGRRRATVYLPSGSRWRDAWRPDRVYEGGQ
                     TITVRAEVHQIPIFVREGSGIDLGDLHQEYKEALEAARERPDLAALDAEVAEWFAARD
                     AIQAGGIPDSR"
BASE COUNT         2158 a         5077 c         4976 g         2228 t
ORIGIN      
        1 gctgggctac ccggtgttct cgcagagcac actgccgctg gacgatgatc tgctcgagga
       61 gctgttcggc gacgaggtcc gcgccggcgt cgtccgcgac ccgctcgaga tccaggacgt
      121 gtggaagaac agctactcgg cgagcacgaa ccacaaggtc tgggccgcga agttcaccgc
      181 gcgccacccg aatctcgtcg cgctggagct gtcgaacttc aagtgcggcc acgacgcccc
      241 gatctacacg gtgatcgagt cgatcatcga gaagtcgggc accccctact tctcgttcaa
      301 ggacgtggac gagagcaagc ccacgggctc gatcaggctt cgcgtcgaga ccatccacta
      361 tttcctcaag cgctacgcgg aggacatgaa caggaagcgg gatgcgggca aggccatcga
      421 cgaacaggtg gccgcgttcg aacggcagct gcgccgccag atgctcctgg acgaggagct
      481 cgcggacatc gccgccaggc agcctgagtt ccccgcgggc cgccgcctgc tgcccgtgct
      541 gccgctcggg cagggcggct cctcgccggg gcgcgagctg cactcgtagc gtcacgaggg
      601 tgccatcttt cacctttctg gtacggcccc ccggctctgg caatcttcac gttttgggta
      661 cggccccctg ggcggtggat ccatcatgga gcccgtccgg tggccccccg atcgaggagt
      721 tgcccgaatg aggtcccgca cgcgcatcgg cagccggtta ggggcggtgg cgatcgtgtt
      781 cctcctggcc gcgttcgcgg aggttcgccc gccggcccgc gcccagtcgt tcgacaggta
      841 cttccccgcc aaggacctga cgacagtcgg cgtctactac taccccgagc actgggatcc
      901 agcccagtgg gagcgcgact tccggaacat ggcggcgatg gggttcgagt tcactcactt
      961 cgcggagttc gcgtgggccc agctcgagcc cgaggaagga cgctacgact tccggtggct
     1021 cgatcgatcc gtggagctgg cggcggagca caagctcggg gtcgtcctgt gcacgtccac
     1081 cgccacgccc ccggtgtggc tggtgcggaa gtaccccgag gtgctggcca ccgccgagga
     1141 cggctcgcgg atggaccacg gatcgcggca gcacgccagc ttctcgagca cgttctaccg
     1201 tgaatactcg ttgaagctga tcgagcagct tgcacggcgg tacggcggcg acgaacgggt
     1261 gatcgggtgg cagctcgaca acgagccgag gcgattcctc gactacggga aggacgcgcc
     1321 ggcaaggttt cgcgcgtggc tcgagaagaa gtacggaacg atcgacgcgc tgaacgcggc
     1381 ctggggcacc gccttctgga gcggcaccta caccagcttc tctcagatca accttccgct
     1441 ccaccgccag tggggcatga acctgcacca gcgcttggac cactaccgct tcgcagacga
     1501 ggaaaccgcg acgttcctcg acgagcaggc aagggtcatc cggaagcacg tcaaggggcg
     1561 gcagtggatc acctcgaact acatcccgat gtacgacgtc ggctacgtcg gcgccagccg
     1621 cgagctcgac ttcgactcgt acacccgcta catggtgtac ggcgcggacc gcgggctcgg
     1681 cccgcgaggc taccgtgtcg gggagtacac gcgcatcgcg atggccaacg acttcttccg
     1741 gccgctgaag gggatgtacg gggtgatgga gttgcagccg gggcaggtga actggggtgc
     1801 catcaaccca cagccccttc cgggcgcggt gcggctgtgg ctctggcacg tgttctctgg
     1861 aggcagcaag ttcacctgca cctatcgata ccgggcgccg ctgtacggct acgaggccta
     1921 ccacgccgga atcgtcggaa ccgacggcgt gacaccgacc gccggcggcc gtgagtacgc
     1981 gcagttcatc gccgacatcc ggcggctgcg cgagaaggcc gacgccgggg ctcccgttcc
     2041 cgctgcctac gccaggcgcg ccaccggcat cctgctcgac gccgacagca tccaggcgat
     2101 cggcctgaac aggcagacca ccgagtggaa caccgagtcg cacgtgctga agtactaccg
     2161 tgccctgaag tcgttcgggg cgccggtgga cttcgttcgc cggggagcgg atcttgcccg
     2221 gtatcccgtt ctcgtggtcc ccgctcacca gatgatcgac gaggaactga tcgccgcgct
     2281 gacccgctac gccgagcagg ggggcacgct gctgctgacg tgccggacgg gcctccacga
     2341 ccggcgcggg cacctgtggg aggcgaagtt ctatgagccg atgtggaagc tggtcggcgc
     2401 cgagatcgag tcgtacgacc tgccgatgcc ccacgcaccc aacacggtgc gcttcggctc
     2461 cgaggacttc gagtggagta gctggggcga ccttctgaaa ccccggcccg gcaccgcaac
     2521 gtgggcgacg caccagggcg acttctacgc gggcaccccc gcggtggtct cacggaagct
     2581 gggcaaggga acggtgacct gggttggcgt ggacacgcgt tcgggtcaac tcgagaagca
     2641 ggtgctggcc aggctcttcg cggaacgagg aatcgcggtc gaaggctatc ccgagggcgt
     2701 cctcgtcgag taccgcgacg gcctcggcat cgccctgaac tactccgaca aggacttcgt
     2761 gatgcccctc ccggccggca cggagatcct ggtcggtgac acgacgctcg aaaccgccag
     2821 cgtgctcgtc tggaaaacgc cccgcggttg attgcggggc cgtaccagaa acgtgacggt
     2881 cttcacgctt cgggggccgt accagaaacg tgaaggcgtt cacgcttttg gtacggccct
     2941 caggacgtga cgatcttcac gctttcggta cggctctacc ggtacttggc caggttgtag
     3001 cgatcggcga gcgcccgccg ctccgcgtcg ggaagtgcct gcatatagga cttgaacccc
     3061 gggcaccacc cggcgtgcca gcgccacagc cgggccaaca acgacgtcgg cttccggtcg
     3121 gccttctccc ggatcttgca cgtcgcgcac cctgcttcgg ccatgtcgca cctccttcgg
     3181 tgcttccgcg cgtctgccgg atcccgatcg caaccgcaag ccagggaggg gcgaccacaa
     3241 gggtcgccct acgccccgac tctcacacaa cccggtgggg cgggccgcaa accggtgggg
     3301 caggccgctc ccggcagtcg ctcaatgccc gatcgccgcg tcgagcgcga ggtaccggtc
     3361 gcggtgcgcc tcgggctcgg cctgcgactt cacgtcgatc cgcatccgct ggtagttggt
     3421 ctccggccga taggcgggcc actccggcag tcccggaccg ttcgggttgc ccgtcttgat
     3481 gaagttcacg aagtaggcct gcatggtctt cgagacctcg tggtcggccg gctcccaggc
     3541 gtagcgcgtg tcgagctcga ggttgcccat ggcgtactgg atctccgccg agtgcgcggc
     3601 gccccgcggc gggcgcgggc cggcgctccc ggccgcatcg ccgcgggccg cggcaggcac
     3661 ggcgggaggc ggctgccccg gcattcccag gtacggcggg cgcacccttg cgtagaggta
     3721 gcggtagacc ggcttgccgc ccgtcttcat gtgcagctcg gtccacttcc aggtgccgta
     3781 ggagacaaag cgtgcgctgg cgaggtgcat cgcagcctcg tacacctcgt ctggcgtcgt
     3841 ggcggcgtac gccttcacga ttgactcggc cttctccccg tagaacttcc ggatcgcggc
     3901 ggcgagcgtt tcggccgtcg gctcgccccc cgccagcacc gcccgcgcgg ggccctcctc
     3961 cgtgttggag ccggcgagca ggggcacctt ggcctgctcg cccgcgagcg cgatctccgg
     4021 cagcgacttc gcgaggaagt agccgtccat cgcggtgctg aagcggatgt cgcgcgcctt
     4081 cccggcggct tccaggatct gctgcgcact catcgcccga agggcggcaa gcgacccggc
     4141 gccggcggac tcggcgaact tcacgccgct cgcctcgacc tcgcgaagcg cccgcggggg
     4201 caggctcgcg attgccgcgc cgctctcgca gatggcgccc gcgatcaggc cgcgcgacaa
     4261 cggcgaggcc atcagcgcac tgaccgagat cgacccggcc gactcccccg cgatcgtcac
     4321 ccgcttcggg tcgccgccga acgcggcgat gttcttctgc acccaccgca gcgccgccac
     4381 ctggtcgagc aggccgtagt tgcccgacgc gccgtgcggc gattccttcg tcagctcggg
     4441 atgcgcgaag aagccgaaga cgttggtccg gtagttcagc gacaccgcga cgatgccctt
     4501 ccgcgccatg cgctcgccgt cgtagcgcgg ctcggatccg tcgccgttct ggaacccgcc
     4561 gccgaagatg tacacgagca ccgggagctt ctcgcggccc gacttcgccg gcgtccagac
     4621 gttcaggtag aggcagtcct cgctcatgcc gtcgccgcgg agccagtagt cggctcccgg
     4681 tgagagccgc tgcatgcagg cgggaccgaa cccgtcggcg ttccgcacgc cggtccagtt
     4741 cttcgggggc tgcggctcgc gccagcgcag gtcgccgacg ggcggctgcg cgaagggaat
     4801 ccccttgaag ctgcggacgc cctcgacggg cgccacggtg gactcgacga tgccgttcgc
     4861 ggtcttgacg cggtcggccc cgaaggccgg caaggcgatg gtgaggagga gagcgagagc
     4921 gggggccaga gcgcgcgcgg agcaggtcat ggatgcctcg attcgggcgc catcatagca
     4981 cccggctcga ggacgcggaa tggtctgccg ccacggccgc cccgagcggc cgcgtcacac
     5041 ggccgtcaga ggaggcggaa cgccggcgta cctgactcga cacgcgccaa gtgatttcgg
     5101 tacggtagta gcgtgacgcg cgtgccgcct ttcgaccgtc ccgcgagcta cgacgacctg
     5161 cttcgccaac cggaccacct cgtggcagag attctcgatg gcgagctgca cgcaaacctt
     5221 cgcccgtcgg ccgtcgccgc cggcagcgcc tttaccctgg ggggcatgct gcgccgcgcc
     5281 tgcagcgccg gagatcccga cggctggcag atcctgaacg agcccgagct gcgcctgggc
     5341 gccgacgtcc tcttccccgc ggcggccggc tggcgcgcct cgcggctgcc ggagctgccc
     5401 cgcaccgcct acttctcgat cgcccccgac tgggtgtgcg agatacccgc cacgtacccg
     5461 gcctcgcttc gctcgcgaag gatggccatc tacgccggtg agggagtctc ctacgcgtgg
     5521 ctgatcgacc ccgtcgcgca gacgctcgaa gtgcgcagcc tgcaggaagg ccgctggagg
     5581 gtcgtggcca cgcacgtcgg cggccagctc gtgcgtgccg tgccgttcga agccctggag
     5641 ctgcagctct ccgcgctctg gcctcgcgag ccgggaaaga cccggggaag gtagaaggcc
     5701 agccctacga acccgacgag ctggacgaac gcggctggcc gagcagtcgc tcgccgccgg
     5761 cctcgcggaa gacctcgcgc ccgcggaggc ggttggcgtc ggcctccgcg agaaccgcct
     5821 ggaacgctgc tctcctgcgc agtgcctcga actcgggccg gtttctgagg agctgtgaag
     5881 gacagtaacc cttcgcgacc gcccgttcga tcagccgcgc cccctcatca tggtcgccga
     5941 ggtcgcacag cagccaggcc tcgaggaaca gggcttccgg gtcgtcctgg atcttgaggc
     6001 cccggagcgt cgcgaggtgt gccagcacgt cgtcgagccg gcgctcgacc cacgcgaaga
     6061 gcgcctcgct ccaggcgcgg aaggccggga tccgcgggat ccggcgcatc tcgtccgccg
     6121 ccagccgcgc ggcctcgcgc cgtcctgcaa agccgagggc gatgacgcgc atcccatcgt
     6181 cggccccggc gacgacgagg gggcgaggga tctcgaggag gcgctcgacg tccccagcca
     6241 tcagcagcgt ctgctcgacg ctggtgggaa cgttcgggtc gagccggcgg gcctcggcgt
     6301 gcgcggcgat cgactcgtcg tagagcccgc agtaccggca ggcgtggacg aggccggcga
     6361 acagctcagg gtcgttgccg ttgagcttcg cttgctcgac cagcctccgc agcgcctgct
     6421 gcggttggcc catgtcggcc tcgaggtgcg cgtagaactt gtgggcgatc gacaggcggg
     6481 ggttgatcgc cagcgcctgg cgcaacgcat tctccgctcg cgcctcgctg tcgggctgct
     6541 cctcgatgaa cttgccgatg acgcgctggc agcggcccag gtgggcccac gccggggcga
     6601 agccggggtc cctctcgagg cagagctcgt agagatgccg ggcgcgcggc aggccgtcgt
     6661 acgtgcggac catctcgttc gcgcgaaggt acagctcgta cgcctctggg tcgtgcggcg
     6721 cgtcaggctc cggcggagcc gcctcggccg tcaacggcag cgagagcgcg tcgaagacgc
     6781 gctggctgat gtcgtcctgc aggtggaaca agtcgcccat cgacgactgc acggtgtggg
     6841 acgtcagcag cgtcccgccg ggtgcctcga ccagctgcac gctcacgcgc agctggtcgc
     6901 ccgcccgcag gagcgttccc atcacgacgc gatcgacgtc ggcttccgag gcaagcgcct
     6961 tcgggtcggg ggcgtcggcg aagcgggcgg ccgtcgcgct cgcgcgcacc accagcgagt
     7021 cgatcccgga gagcgaggtc gcgatcgcgt ccggcaggct gtaggcgagg aagtcggtct
     7081 ccggatcggg acgcagcacg cggaacggca gcacgacgag gcgcagcagc gggcgcgcca
     7141 gcgtcggcgc gctggcgcca tcgacgtggc ggatcccgcg cagctgccgg gccatggctt
     7201 cggccgacgc cgggcgatcc tccggacgct tggcgagggc gcggcggatg acgcggtcga
     7261 cggcggccac ggccggcgac ccggtcaggg cgggaggctg atcgtgcagg gtggcgtgca
     7321 gcacctcgac cgcgctggcg ccgtcgaagg ccggccggcc cgccagcatc tcgaagagga
     7381 tggcgcctgc cgcgaacagg tcgcagcggg cgtcgaccgt ctcgcccctc acctgctcgg
     7441 gcgccatgta gtgcggcgtg ccgatgatcg agccaatcgt ggtgaggccc gtcgcgctcg
     7501 cgtccaccgc cccggcctcg gggcgtgcga ggccgaagtc gagcaccttc acgccgtgcc
     7561 gggtcaggaa gacgttcgac ggcttgaggt cgcggtggac gatcccgcgc gcgtgcagct
     7621 cggagagggc ggccaggatc ccgagcgcaa ccggcagcgc ctcggagacg ttcatcgggc
     7681 cgcggcgcag ccggccggca agcgactctc cctcgagcag ctccatcgcg atgaacagct
     7741 cgccatcatc ctcgccaacc tcgtagatct ggcagacgtt gggatggctg acgctcgctg
     7801 ccgatcgggc ctcgcgccag aagcgctgcc gcgcggcctc gtcccgggcc ggcgaggcca
     7861 tcgtcttcag ggcgacgctt cggccgagcc gctcgtcgcg cgcggcatac accacgccca
     7921 tgccgccctg cccgagcatg gaggtgatgg cgtagcgtcc gattcgcgcc ggcagaccac
     7981 taccggcacg cttctcgatc gacccttcat tcacggattc atcgatcata gcgcgggaag
     8041 gggccgcggc ggcagccgca ggcgcagttc gaagctcgac cgcccttccc cgcgaaaagc
     8101 acttgtcacg cgtcgcgctt cactcttcgg cttcgctcag ggagccttgc cgagccgccg
     8161 gttcccacgg ctcggcggtt gcgcccgccc cgcgccgctg tcacaatgcg cggcatgtcg
     8221 atcttcagaa ccatcgcttt ctttctgact ctgattgccg cctcggtttc cgcgggccag
     8281 cagcccgccg cccccgcgcc cggctccagg caagttgttg ccccggcagg ggcgttctcg
     8341 gcggaacggc tcgcgcggct cgatcgcgtg cttcagcagt acgtagacga ggaccgcgcg
     8401 gcgggcatcg tcgcgctcgt gctgcgcgac ggcaagcccg tctacgaccg cgcgttcggc
     8461 tggagcgaca aggaagccgg gcaccggatg acgaccgaca cgatcttccg catcgcgtcg
     8521 cagagcaagg cgctgaccag cgtggcggtg ctctcgctga tggaggaggg ggcgctctcg
     8581 ctgaacacgc cggtgagcgc gttcatcaag agctttgcga agacgagcgt ggtggtgaag
     8641 aaggacgggg acgtcgccac ggttgcggcc aggcggccga tcaccatccg cgacctgctg
     8701 acgcacacgg cggggatctc ctacggcacc gacgcgacgg tggcggcgct gtacgagccg
     8761 aaggggctcg ggccggcggc aggctggggg tggtacaccg ccgacaagga cgaaccggta
     8821 tgcgacacga tggagcggct cggcacgctg ccgttcgtcg cgcagccggg cgaagccttt
     8881 gtctacggct acaacaccga tatcctcggc tgcgtagtgg agcgggcgtc gggcatgccg
     8941 ctcgatcggt tcatcgcctc gcggatcacc ggcccgctcg ggatgaagga cacgcacttc
     9001 ttcctgccac ccgggcagcg ggcgcggctg gcggcggtct acggcaacgg gcccgacggg
     9061 aagatcgcgc gcgcccccga gggctcgcgc ggccagggcc actacgtgga tggaccgcgg
     9121 aagagctttg ccggcggcgc gggtcttctc tcgaccgcgc acgactacgc gcggttcctc
     9181 gagatgatcc gccggggcgg cgaactggac ggcgtccgca tcctctcgcc ccgcaccgtt
     9241 gcgctgatga cgaccaacca ggtaggcgat ctctaccgga cgccgggcct gggcttcggc
     9301 ctcggcttcg aaaccaccga gcgttacggg gcgagcggca tggcctcggt cggagctttc
     9361 ggctgggggg gcgcctacgg aacgacctac aaggtcgatc cccaggcgcg gctcgtgctc
     9421 gtcatgatgg tgcagctcct tccgaacacg accgacatcg gccagaagtt cccgaacctc
     9481 gtctaccagg cgctgatgga gccggtggaa tgaggacgct cgcctccgtc gccgtcgtgt
     9541 cgttgctgct cggccttccc gccgcctcgc gggcggcggc gccggcgcct ggcgtgccgg
     9601 cgaacggggg tgtgaaggtc cccggcccct gggtcgccct ccacgtcctg aactacgaga
     9661 ccgatgccga cctcgcggcg ctcgaacagc agctgcccgc gttggccgcg cggggcgtga
     9721 acgtcctcgt cctcgaggtc gactacgcct tcgagtcgct ggccctcggg atgccgggcg
     9781 tgctcggcca cctgttcacg atctggtcga agcagccgaa tgtcgccgac tggcctcccc
     9841 tcgcggcgaa cgccagcctg gtgcggcagc tggcaggagg caagggccgc tgaaccgccg
     9901 tcccctggtg tcctcctagc gcgccaggtc tgtcggcacc ccgcccttca cgcggcttct
     9961 ctcggcggcg tccatgaacg ccatgatctc gagggtttct tccgggggaa cggggggctt
    10021 gccggtctgg aagaacgcga cgatctgccg cacgaggggc acgtaggaga cgtccttcac
    10081 ggggaggtag gtcacggcct tgtcgccgta gaccagcgcc ccgtagtcct gcttgcccgt
    10141 ccgcagcccc ttcaccgtgc cgatccggcc gtccttccac acgccgaccg cgaggtcgta
    10201 gtcggcgttc gacgtcatgg tcaccgaggt gcacccgggt cccatgagcg tgtagagagt
    10261 ctccaccgcg tggatgccgt accagaacag gcctgggttg gtcggctcga gcgaggcagg
    10321 cccgtgcgcc tcgacgccga gcacctgccc gcgcgccggg tcggccagga actcctggta
    10381 ggcgctcgcg aaccgcagcg acgaggagct gaaccacggc gtccccgact cctgcgccag
    10441 gcgcgcgatc tccttggcgt cggcaaggct ggccgccatc ggcttgtcga tgaagagcgg
    10501 cttgcgccgc cgcagcactt cgcgggcctg ctcgaggtgc accctgccgt cgacgctctc
    10561 gagcaggacc gcgtccacca ggtcgcacag ctccgcgatg gtgccgacga tccgcacacc
    10621 gtaggtggat tgcagatcgg cagtgaactt gtccacgcgg gtcgcgctgg ccgggacgtc
    10681 ggggctgcct cccttgaagg cagccacgac ctttgcgccg ggaacgtggc cggggctcga
    10741 ggcgtcgttc agcgacctcg tgaaggcgat gacgtgcgac gtgtcgaggc cgatgatccc
    10801 gatccgcagc tccgcggccg aggcaacctc accaagaaca cacatcagca gccccgccag
    10861 gatcagtacg cgcttcatct cccggtcccc ctactctgac ctacgactgt cctaaagaac
    10921 catctcccag cccttctcgt actcccgctc ccagtacttg gccgcctccg ggtcccccac
    10981 cacgtggccc gtcgccctgt cgatgttgat gctcctgccg gtccggaacg cgatgttgcc
    11041 aagctggcag agcagggtgc tgacgtggcc ctcgtggatg tcggcgttca ggctggcggt
    11101 tccgcggatc gagtcgagga agttgcggac gtgcacgacg tcgaggctgt cggcggggtt
    11161 ggtcgggttc cggggatcga tgtcggtcgc gctcttcact tccctgacga ccttctcgtc
    11221 gagatcctgg acgaggtacc cgttgccgtc caccagcagg ctgccgttct cgccgtggaa
    11281 gatcgccccc gcgcctgagc cgtcgatcgt gcgccccgag cagctgcgcc cctcccacgc
    11341 gatcgacacc ttctcggcgt agtcgagcgt gatcacctgg gtgtcgggcg gctcccagtc
    11401 gtccacgaag tggaagcgcc ccccagagga accgacgcgg acggggtact cgacgccaag
    11461 gccccagcgc atcaggtcga gcatgtgggt gccgttgttc agcgcctcgc cggtccccca
    11521 gtgccagaac cagtgccagt tgtagtgaac caggttgtcg cggaacggcc ttcgcggcgc
    11581 cggcccctgc cagagctcgt agtcgagcca gtcgggtacg ggcgcttcct ttccgaaccc
    11641 gatcgacttg cggttgttgg tgtaccaccc cttggcgaag tacggccggc cgatgatgcc
    11701 gccgcgcagc tccttcaccg cctcgacgac gttcggccac gaccgccgct gcgtcccgag
    11761 ctgcaccttc ctgccgtact tccttgcggc cgccaccagc atctccccct cggcggggct
    11821 gtggctgcac ggcttctcga ggtacacgtc cttgccggcc ttgcacgcga gcagcgtggc
    11881 cggggcgtgc cagtggtcgg gggcggcgac gaccagcgcc tgcacctcgg ggtcctcgag
    11941 gcagcggcgg aagtcgttgg tggtcttcgg cgcgttgccc tggatgccgg ccaccgccgc
    12001 ggcggtcttc tcgatggccc ggcggtcgac gtcgcacacc caggccacgc ggcagtcggg
    12061 ctgtttgccg aaggtgccgg ccagcgagtg gccgcgcccg ttcagcccca tcacggccac
    12121 gtggatgcgg tcgttcgcgc cggcaacctg ggcccggccc ctcgcggtga acccggggcc
    12181 gaggccgacg aacgcggcgg cggccgcgcc gcccgaaacg ctcctgatga attcgcgtcg
    12241 cgatcgcttc atgactcccc ccttagcgct cccgctcagg ccggggattg taccactcga
    12301 ggaagcgcag tcagaggcgc acaggcggga cccctgccgg ctcacagtcg ggcacttcac
    12361 cggctgtccg ggatccctcc cgcctggatg gcgtcgcggg ccgcgaacca ctccgcgacc
    12421 tcggcgtcga gggccgcgag gtcgggccgc tcgcgcgcgg cctccagcgc ctccttgtac
    12481 tcctggtgca ggtcgcccag gtcgatcccg ctgccctcgc gcacgaagat cgggatctgg
    12541 tgcacctcgg cgcggacggt gatggtctgg ccgccctcgt agacgcgatc ggggcgccac
    12601 gcgtcgcgcc agcgcgagcc ggacggcagg tacaccgtcg cccggcgccg ccccttcttc
    12661 cagatcggtg agaccaccag atcggctccg taaagatagg tttcccagtg ggtccaggcc
    12721 tggtgtgccg aggggtcggc caggaacagg ggccgaacga tcggcatccc ggtggcggcc
    12781 gcctcgcggg cgtggcggta ggtatagtcg gccagccgca ggtggaggcg cgcgtagagc
    12841 cgccaggtcg cgatcagccg ctcgtcgtag cggggtttgc gcggcaggtt ccagaagccc
    12901 gtgttacgcg tcggcccgac ctccatgatt ggcgtgaaag cgctgaaggc gagccaccgc
    12961 gcgcacacct cctgttccag cagctgccgg ttgtacccgc aggtgtcgga gccccagatc
    13021 gggtacccca tcaccgacgc ccgctgcacc gcgatgatcg acgcgcgcag gccctcctgc
    13081 gtgccgaaga cgtcgccgcc ccagaacacc ccgtaggggg cgctgccggt gtaggccgcc
    13141 ctgggcatca gcaggaagtc gtcgccgcgg tgcttcctcg cctcttcgta cgccgcccgg
    13201 aggaacacca ccgggtaggc gctccggtgt tcgcgcagcg aggtgccgtc gaacacgtgg
    13261 agcggctcct gcgggaagtc ctcgtcggcg cgatcgagtt tgaaggccgc cacccccagc
    13321 ttcagcagct tcgagaaccc ttcctgccag aacgcgcgcg cctcggggtt cgtgaagtcg
    13381 gccagcgggt agttgttgaa gttcggccag cggtggcccg gcatgtggta gccggccgcg
    13441 agggccgtgc gcgccatctg tccctggaag aacggcccaa tccacaacat cgtccgggtg
    13501 ccctggcggc cgagccaccg caccatctct tcgaagttcg gaagccgctt ctcgtcgatc
    13561 tcgaagtcgt cgtagccatg gcggcccgac ccccacggcc ggtcgatcca gtacacgccg
    13621 ttcggaatcc cgtaggcctt catcatgagg acgtcttcca tcacgtccgc gttgaagggc
    13681 cccttcaccc gcgtgccgtc gtagtagcgg tcgcggtgca cgtgctcgtc gcgccagcgc
    13741 cacggcgtaa aggtccactt ggggggcagg atcggaggac ccgcgtcgat cgcgtgatcg
    13801 cggacgatga cagccgggtc gggatcgagg tagagcttca tctcgagcga cgttccctcg
    13861 aaggcaatcg tcacccggtc gcgcgcgtcc acgcagaagc cgaagtgccc ccgcgtcgtg
    13921 cccttcacga acacgccgta cccccgggac gacaagtaga acggcgcgta cagcgaggtc
    13981 gtccgcttca tgatcatcga cacgcgctgg ccgcgcaggt tcatcgcctt ctcgaggccg
    14041 ggcagccacg agcgctcctg cgggccgtcg acgacgcgct ccatcaggcc cgtgaagtac
    14101 tcgtcaggcc ccgcgtcgat cgccaggccc cacccgcgca cgccgcggga cggctcgacc
    14161 gtcagccgaa gtgcgcggtg gttgccgtcg gacgagacct ccaggcggac cgtccgcccg
    14221 tcggccatct tcgcctcgcc gacgcccccg gtgccgactc gcgaggtcag cgaggccggc
    14281 agccgggtac gcccgcgcgc ggtctcgtag tagaagcggt cgatccgtag cgaggtgcgc
    14341 cgcgaagccg aatgcgccct caggggaacc ggggcgggaa tctccagcga ggcgccggca
    14401 gacctggcgc cggcggcggc gggaagaggc tgcgagacg
//
LOCUS       DAOPQX010000053        14436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4909_length_14436_cov_4.973437, whole genome shotgun sequence.
ACCESSION   DAOPQX010000053 DAOPQX010000000
VERSION     DAOPQX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14436
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4909_length_14436_cov_4.973437"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..>786)
                     /locus_tag="PKK95_04680"
     CDS             complement(113..>786)
                     /locus_tag="PKK95_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17539.1"
                     /translation="VMRDVLERFAPGQVARYDDEFEVRAFGDNITLWGTPVVLLETGA
                     FGGPTPDEDLVRLNFVALMAALDEIASLRVHDADPARYESLPVNQSGLFALLVRGAAI
                     VTGTGIEPFVGDVGIGVLRAVRGEIDGEREFVRVLRVEDLGDLRVFSALETIDAVGMY
                     VAPAWDPALKPGVTIDVPDWTTVKSRTPLAVGTPANLVLLRQLGENRYRVERGIEAEE
                     VLGKR"
     assembly_gap    787..796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(798..1574)
                     /locus_tag="PKK95_04685"
     CDS             complement(798..1574)
                     /locus_tag="PKK95_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOC17540.1"
                     /translation="MFSPMRHSRLGLLAATLFLALAPARPISSQAPAAEPGLVAPLSP
                     EALSRTWLEERRDYGPPALVDHALVLSELKRISAASEDLFTLEEIGRSVEGRPILRLA
                     FGRGPFHVLLWSQMHGDEPTATPALLHMAEHVRRHREEPAIARMLDELTVHMVPMLNP
                     DGARRFQRRNAQGIDINRDALLLQSPEGRALKALRDRVQPGLGFNLHNQNWRTAAGKT
                     GKPASFALLSVASDEARTVTPGRVLTKKTCGSHGARTGER"
     gene            complement(1597..2493)
                     /locus_tag="PKK95_04690"
     CDS             complement(1597..2493)
                     /locus_tag="PKK95_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOC17541.1"
                     /translation="MRVVIAGGTGFLGRALGRALLDQGHEVVVLTRSRKAAGVSAVVW
                     SPGGEPGAWAEVIDGAGAVVNLAGESIGAGRWTDARRRAIRESRVTATRSLVEACLQA
                     KRPPRVFVSGSGQGYYGDRGDEVLTEASGPGRDFLAGVCAEWEAEAQKVSSVARLAVL
                     RSALVLDRDEGALPRMVLPFRLLAGGPIGSGRQFVSWVHRDDWVGLAVRAVADSRFEG
                     PVNAGSPNPVRNAEFAKAIGKALGRPSWIRTPATALRAALGEMADALLLSSQRMVPAK
                     ALDLGYEFRHPEARGALARILG"
     gene            complement(2624..4810)
                     /locus_tag="PKK95_04695"
     CDS             complement(2624..4810)
                     /locus_tag="PKK95_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOC17542.1"
                     /translation="MEQPRLLDRPASAVEHLTGVVERVVFHNDENGFTVLRLSVRGRR
                     DLLTVVGHAPAARPGEFLQAEGTWKHDRTHGLQFEAASMQAVTPTTLEGIEKYLSSGL
                     IKGIGPHLAGKLVEAFGTRVFEVVEHQPERLRTVGGIGRTRAESITSAWRDQKSVRDI
                     MLFLHSHGVGSSRAVRIFKTYGHEAIPLITENPYRLAADIRGIGFLTADRIAASLGID
                     PQAMIRARAGIGYALTEAMDDGHCGLPLAVLKETASRLLEIPAPLIERAIQMELDAGT
                     IAADQVEGREAIFLAGLYSAERAIALRIRSQATGPLPWPSIDADAALGWAERRLGMTL
                     APMQREAFSMALRTRFLVITGGPGVGKTTLLNAILAVLAAKRVRMALAAPTGRAAKRM
                     SEATGREAKTIHRLLEVDPRTGGFRRQESNTLDCDLLVVDETSMVDVPLMHSLMRAVP
                     PRAAVLLVGDVDQLPSVGPGRVLGDIIDSGAVPVVRLSEVFRQASASRIIVNAHRINA
                     GEMPELTPSGADDFYFWEADEPEKAAQRLLQAVCERIPARFGLDPMRDVQVLCPMIRG
                     VLGARSLNAELQKRLNPGREPRLERYGWTFSPGDRVMQVENDYDKDVYNGDLGFIRSI
                     DPSLSHLTVDFDGRPVDYDVSDLDQLALAYATTIHKSQGSEYPAVVIPVSTQHYAMLQ
                     RNLLYTAVTRGRRLVVLVGQRKALGIAVSDRRLRRRWTMLRKWLTR"
     gene            5035..5730
                     /locus_tag="PKK95_04700"
     CDS             5035..5730
                     /locus_tag="PKK95_04700"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC17543.1"
                     /translation="MKRPLDSREAILAAAVAEFALHGFRGATVDAIARRARLNKAMIY
                     YHFDDKLALYLEILRGVFTAVRDSSAAIVASPVAPPQKIAAFIDAFEAEADKRPYLPR
                     LMMLEIADGARHLDPPTLRVMLGIFQNLRAILEEGRAAGVFRPVNPVVTYFSIIGPMM
                     FFRAAAPVRELVSRLDIEESFRMDTAAFADHLKVAALQTLAPSVGAPANAAKPRPARA
                     RGKGRAAKTGDRT"
     gene            5727..6737
                     /locus_tag="PKK95_04705"
     CDS             5727..6737
                     /locus_tag="PKK95_04705"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC17544.1"
                     /translation="MRTHALAVTLALASTFTVACRSNEPAGSVRASGTVEATEVQVAG
                     LVGGRLLELKVAEGDRVEAGQVVAQLDVADARLALQRAQAERAQADAQLRLLLAGARP
                     EEIRQAEAQLRAAEAEAAAAREDLRGATADLQRFDTLLANNSGSRKQRDDAATRVDVM
                     GARVRAAEQRSQAAAETLARLKKGARAQEIDAARARLGVVDAQIATLEKSIADATIAA
                     PVAGIVTQKLADVGEILAPRAPVVSIVDLDHAWANIYIDEPLVPRVRLGQQATLYTDA
                     GGPGIEGKVTFVSPRAEFTPRNVQTAEERSKLVYRIKVSVDNREGILKQGMPVEAEVP
                     LS"
     gene            complement(6901..8556)
                     /locus_tag="PKK95_04710"
     CDS             complement(6901..8556)
                     /locus_tag="PKK95_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC17545.1"
                     /translation="MIAISRRARAASIVVLALAVSPAAAAAQVREPEPSERILPLTLE
                     EAVRRAVEHNPELAIVRLDTQAGAARVGESRTAFTPLFSASVGRSSTATPPASFLAGD
                     SGVDVDDLFSSFGVRQRLPWGSGTWQVSWEMARTSTNSPISNFDPSLESGFQIAFSQP
                     LLRDRSVDRARYQYAIAKRNLESSELRFRESVTQTVAAVKQAYWTLRATQANISVAQR
                     SLELAEELARQTRIRVDAGQTPPLDLTQAEAEVAQRRENLIRAHATAGDAEDALRLLI
                     ADPSDASFWQMHLQAVEQPTPLREQPDVDAAVARALGERYDIARAGHQLENARTTVEF
                     YGNQRLPDVRLETSWRGNGLAGTQFLRSGGFPGTIVGRRSLGLGEALSQVFRSDYPAW
                     SVGLTVNYPLGQSYEEAGRVRAEIERKQAEQRIASLRLRVAEAVRRAGRQVRSTAERI
                     DAARAGARLAEERLNAEQRRYQAGLSTTFLVTQAQRDLLQAQVNLLETMLAYESALVN
                     FEAIQQAPPLAVGDTVQVEGATIVALPAPAPRGMFRANTSGLQ"
     gene            complement(8571..11648)
                     /locus_tag="PKK95_04715"
     CDS             complement(8571..11648)
                     /locus_tag="PKK95_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC17546.1"
                     /translation="MKFTDLFVKRPVLAIVVNLVILIAGLQSIRALSVRQYPRSDIAV
                     IRVSTAYIGAGADLVRGFITTPLERVIASADGIDYMESSSAQGLSTITVHLKLNYDTN
                     AALTQIQAKVAQVRNDLPPEAEAPIIELETADTQFAAMYLGFSSPDLDQTQITDYLRR
                     VVQPKISAVSGVQRADILGDRTFAMRIWLKPDRMAANGISPSAVQEALSRNNYLSALG
                     RTKGSMVSVNLVANTDLRTPEEFRQLVVKEADGAVVRLGDIADVVLGAENYDQNVRFN
                     GETATFMGVWVLPTANSLEVIARVRDTVDALRAELPVGMKVGIPYDSTEYISDAIGEV
                     LTTLSETLLIVVVVIFLFLGSVRSVLIPVVAIPLSLVGAVFLMLVAGFTINLLTLLAI
                     VLSVGLVVDDAIVMVENIERHLHQGKRPVRAAMDAAHELVGPIIAMTITLAAVYAPVG
                     IQGGLTGALFREFAFTLAGAVVISGVVALTLSPMMGAKLLTAGATERGFAGMINRHFD
                     VVRGWYTRALGATLAYRPVVLVLWVIVASLIVPFYMFSQRELAPSEDQGVVFTIIQAA
                     PNATIEQTSLFAGEIDRVFRSVPEAAGTFQIALPSGGFGGMVTKPWSERTRTAQELQV
                     AVAGPFSKIPGIRAIPLTPPPLPGGGDFPVDLVIASPAEPQQVAEIAGQLVQKAFASG
                     LFLFADSDVKFDQPQAEVVFDRDKLRSQGVDLGQAGRDLSVMLGGDYVNRFSIQGRSY
                     KVIPQIERAERLTPDQLRQIYVTGSNDRLVPLSTFATLRQTVEPRELKKFQQLNAVRV
                     QGVVPPGVALDQALGFLEREARAALPAGVSIDYAGESRQLRTEGRAFLATFLLSAILI
                     YLVLAAQFESFRDPFIILAGSVPLAVSGALLFSFLGLTSLNIYSQVGLITLVGLVSKN
                     GILIVQFANHLQEQGRQKVAAVIEASGTRLRPILMTTAATVAGHFPLVLATGPGAGAR
                     NSIGMMLVTGMVIGTAFTLFVVPAIYLLVARRRTATAVERPVTDTGTGLAEAV"
     gene            complement(11648..12775)
                     /locus_tag="PKK95_04720"
     CDS             complement(11648..12775)
                     /locus_tag="PKK95_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC17547.1"
                     /translation="MAKRMILMLIVTAVFVGALGFVKFRQIQTAIGEAASFQPPPEAV
                     TTVVVSEERWPATLTAIGTVAAVQGVTVSADLPGTVERIEFESGQAVREGQVLAVLDT
                     RQEQAQLAAVEARRELARTNFERMQGLLNERVISKAEFDQATAEYRRTDAEAGEIRAA
                     IQRKTIRAPFSGILGIREVNLGQYLAGGDPLVTLQALNPIYVNFGVPQQAATQVRVGR
                     TVRVRTDGSPAIELAGRVTAIDSVVDEATRNIEVQATLGNPERALRPGMFVQAELFLG
                     REDPVKALPASAISYAPYGDSVFVVAELTDEQGRRYRGVRQQFVKLGPARGDQVAVVS
                     GVNPGDEVVTSGVFKLRNGAAVEVDNSVRPANSPAPQPGNS"
     gene            13043..14386
                     /locus_tag="PKK95_04725"
     CDS             13043..14386
                     /locus_tag="PKK95_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOC17548.1"
                     /translation="MGPQFSSSTEQGRTPCGRPLQPPDPEGASSNDPEVLRAALLREA
                     AERRRAEVLAKIQTEVARVTLDLLVREPDIEGFFGALTQTMVEKGGNHGCGVWLIDDD
                     RQDCRLWMACLDGQLVTPRSAGWDAMALPRDSMSAHLFAYSPGWQQTVEYAGGDTRLP
                     EPVREFHRREGIDKVIVTPLILGSHNLGWIAITAYDISGCDKWWHVALVEAVARQATL
                     ALYQSRLAERTQLEERRKAVLEERNRLARDIHDNLAQGFAAILMQLQAALRGAPRLEA
                     GLARKLETAVELARTHMIEARRSVGTLRPNVGDGEDIGQALARIAALAQRTTGTPIDV
                     ALDDLPRFGDVVEREIVAIVQEALNNAVRHSRAGRIRVEAGPVRSIGLRVSVSDDGRG
                     IPRESSGSGFGLTSMQERAERIGASLTIVTAPRSGTEVVLAWEPSSLPTQVHVAG"
BASE COUNT         2125 a         5226 c         5053 g         2022 t
ORIGIN      
        1 tgaccgagac gaggatcgtc gtcttcgccc cgtgacaccc gctcacggct tgctcaaccc
       61 gaacgaacgc aggcgtagag ccgggctcta gccctgctcc ttcgagccgc gatcaccgct
      121 tccccagcac ctcttcggcc tcgatccccc gttccacgcg gtagcggttc tcgccgagct
      181 ggcgcagcag caccaggttc gccggcgtcc ccaccgcgag cggcgtccgc gacttcaccg
      241 tcgtccagtc gggcacgtcg atggtgacgc cgggcttgag cgcggggtcc caggcgggcg
      301 ccacgtacat gccgaccgcg tcgatcgtct cgagcgcgct gaagacgcgc aggtcgccga
      361 gatcctccac gcgcaacacg cggacgaact cgcgttcacc gtcgatctcg cccctcacgg
      421 cccgcaacac gccgatgccc acgtcgccga cgaacggctc gatgccggtg ccggtgacga
      481 tggccgcgcc gcgcacgagc agcgcgaaca ggcccgactg gttgaccggc agcgactcgt
      541 atcgggccgg gtcggcgtcg tggacgcgga ggctggcgat ctcgtcgagg gcggccatca
      601 gcgccacgaa gttcagccgc acgagatctt cgtcgggcgt cgggccgcca aacgcccccg
      661 tttcgagcag caccaccggc gtgccccaga gcgtgatgtt gtcgccgaac gcccgcacct
      721 cgaactcgtc gtcgtagcgc gcaacctggc cgggcgcaaa cctctccagc acgtcgcgca
      781 tgacggnnnn nnnnnntcta cctctccccg gtgcgcgccc cgtgcgatcc gcaggtcttc
      841 ttcgtcaaca cgcggcccgg cgtcaccgtg cgggcctcgt ctgacgcgac cgagagcagc
      901 gcgaacgacg ccggcttgcc ggtcttgccg gccgccgtcc tccagttctg gttgtgcagg
      961 ttgaacccga gcccgggctg cacgcggtcg cgcagcgcct tcagggcgcg gccctcgggc
     1021 gactgcagca gcagggcgtc gcggttgatg tcgatcccct gcgcgttccg ccgctggaag
     1081 cgccgcgcac cgtcggggtt cagcatcggc accatgtgca cggtcagctc gtcgagcatc
     1141 cgcgcgatgg ccggctcctc gcggtggcgc cggacgtgct cggccatgtg cagcagcgcg
     1201 ggggtggccg tgggctcgtc gccgtgcatc tgcgaccaga gcaggacgtg gaagggtccg
     1261 cggccgaacg cgagccgcag gatcgggcgg ccctccaccg accggccgat ctcctcgagg
     1321 gtgaacaggt cctcggaagc cgccgagatt cgcttcaact cggaaaggac gagcgcgtgg
     1381 tcgacgagcg ccggcggacc gtagtcgcgg cgttcctcga gccaggttcg cgagagcgcc
     1441 tcgggcgaga gcggcgcgac gagcccgggc tcggcggcgg gcgcctggga tgagatcggc
     1501 cgggcagggg cgagagccag gaagagcgtg gccgcgagca ggccgaggcg ggagtggcgc
     1561 atcgggctga acatgggggg agtataggcc cgcccgtcaa ccgaggatcc tcgccagtgc
     1621 gccgcgggcc tcggggtggc ggaactcgta gccgaggtcc agcgccttcg ccggcaccat
     1681 ccgctgactc gagagcagca gcgcgtctgc catctcgccg agcgccgcac gcagcgcggt
     1741 cgccggggta cggatccagc tgggccggcc gagtgccttg ccgattgcct tcgcgaactc
     1801 ggcgttccgg acggggttgg gcgatccggc attgaccggg ccctcgaacc gcgagtccgc
     1861 caccgcgcgg accgcgaggc ccacccagtc gtcgcggtgg acccacgaga cgaactgccg
     1921 gccgctgccg atcggccccc cggccaacag ccggaacggc agcaccatcc tgggcagcgc
     1981 cccctcgtcg cggtcgagaa cgagcgcaga cctcagcacc gccaggcggg cgaccgacga
     2041 taccttctgc gcctcggcct cccattccgc gcagacgccc gcgaggaagt cgcggcccgg
     2101 gcccgacgcc tcggtcagca cctcgtcacc gcggtcgccg tagtagccct gccccgagcc
     2161 gctcacgaag acccggggcg gtctcttcgc ctgcaggcag gcctccacca ggctgcgggt
     2221 cgcggtgacc cggctctccc tgatcgcgcg ccgccgggcg tccgtccacc gccccgcgcc
     2281 gatcgactcc cccgccaggt tgaccaccgc cccggcgccg tcgatgacct cggcccaggc
     2341 acccggttcg ccgccgggac tccacacgac cgcggacacg cccgccgcct tgcgcgatct
     2401 cgtgagcacg accacctcgt gcccctgatc gagaagagcc cgcccgagcg cgcgcccaag
     2461 gaatccggtt ccgccggcaa tcaccaccct catcgcacgc ctcgaacctt caccattcgc
     2521 gcgacaccgg aaccgcagag accgccggag ccacagcggc tacagcaagc acaactacag
     2581 caagcacagg cgagacgcct gtgccactgg gtccaccggg gcgctaccgg gtcagccact
     2641 tgcgcagcat cgtccagcgt cggcgcagcc ggcggtcgga gaccgcgatg ccgagcgcct
     2701 tccgctgccc cacgagaacg accagccgac gcccccgtgt cacggccgtg tagagcaggt
     2761 tccgctgcag catcgcgtag tgctgcgtcg agaccgggat gacgacggcc gggtactccg
     2821 agccctgcga cttgtggatc gtggtcgcgt aggccagggc gagctggtcg agatcggaga
     2881 catcgtagtc caccggccgc ccgtcgaagt cgacggtgag gtgagaaagg gacgggtcga
     2941 tcgaccggat gaaccccagg tcgccgttgt agacgtcctt gtcgtagtcg ttctccacct
     3001 gcatcacccg gtcgcccggc gagaaggtcc acccgtagcg ttccaggcgc ggctcgcgac
     3061 ccgggttgag ccgcttctgc agctcggcgt tgagcgagcg ggcgcccagc acgccgcgaa
     3121 tcatcgggca cagcacctgg acgtcgcgca tcgggtcgag gccgaagcgc gcgggaatcc
     3181 gctcgcagac ggcctgcagc agccgctgcg ccgccttctc gggctcgtcg gcctcccaga
     3241 agtagaagtc gtcggccccc gagggcgtca gctcgggcat ctctccggcg ttgatccggt
     3301 gcgcgttgac gatgatgcgg ctcgcgcttg cctggcggaa cacctcggac agccggacga
     3361 cggggacggc gcccgagtcg atgatgtcgc cgaggacgcg tccgggcccc accgacggca
     3421 gctggtcgac gtcgcccacc agcaacaccg cggcccgcgg cggcaccgcg cgcatcagcg
     3481 agtgcatcag cggcacgtcc accatcgacg tctcgtccac caccagcaga tcgcagtcga
     3541 gcgtgttcga ctcctgccgc cggaacccgc cggtccgcgg atcgacctcg agcaggcggt
     3601 ggatcgtctt cgcctcgcgg ccggtcgcct cgctcatgcg cttggcggcc cggcccgtcg
     3661 gcgccgccag cgccatcctc acccgcttgg ccgccagcac cgcgaggatc gcgttcaaga
     3721 gcgttgtctt gccgacgccc ggcccgccgg tgatgaccag gaaccgggtg cgcagcgcca
     3781 tgctgaacgc ctcgcgctgc atcggcgcca acgtcatccc aaggcgccgc tctgcccagc
     3841 cgagcgcggc gtcggcatcg atcgacggcc acggcagcgg cccggttgcc tgcgaccgga
     3901 tccgcagcgc gatcgcccgc tcggccgagt agaggcccgc gaggaagatg gcctccctgc
     3961 cctccacctg gtcggccgcg atcgtgccgg cgtcgagctc catctggatc gcccgctcga
     4021 tcagcggcgc ggggatctcc agcagccgcg acgccgtctc cttcaacacg gcgagcggca
     4081 gcccgcagtg gccgtcgtcc atcgcctcgg tgagcgcgta cccgatgccg gcgcgggccc
     4141 ggatcatcgc ctgggggtcg atgccaaggc tggcggcgat gcgatcggcg gtcaggaagc
     4201 cgatgccccg gatgtcggcg gccagccggt aggggttctc ggtgatgagc gggatggcct
     4261 cgtggccgta ggtcttgaag atccgcacgg cgcgcgacga gccgacgccg tgcgagtgca
     4321 ggaagagcat gatgtcgcgg acgctcttct ggtcgcgcca ggcgctggtg atgctctcgg
     4381 cccgggtgcg gccgatcccg ccgaccgtcc gcagccgctc gggctgatgc tcgacgacct
     4441 cgaagacgcg ggtcccgaac gcctcgacga gcttcccggc caggtgcggc ccgatgccct
     4501 tgatgaggcc cgacgacagg tacttctcga ttccctcgag ggtggtgggc gtgaccgcct
     4561 gcatcgaggc ggcctcgaac tgcagcccgt gggtgcggtc gtgcttccag gttccctcgg
     4621 cctggaggaa ctcgccgggc cgggcggcgg gggcatgccc gacgacggtg agcaggtcgc
     4681 ggcggccgcg gaccgagagg cgcaggacgg tgaagccgtt ctcgtcgttg tggaagacga
     4741 cgcgctcgac caccccggtg aggtgctcga cggccgaggc ggggcggtcg agaagtcgcg
     4801 gctgctccac cgggacaggg tacgacagga ttcgggattc ggggttgaaa cgtacgtgac
     4861 agacggagcg gcttgtcacg tcaaacgccg cggaggcgta cgcagagatc tcggatcatt
     4921 tcgagatctc agcgcctctg cgcctctgcg cctctgcgtt gaacgtgacc aggttgaatc
     4981 gggcttgaca gcagccgctc gcggttgtta actaactggt tagtgagttc cgctatgaag
     5041 cgcccgcttg attcccgcga ggccatcctc gccgccgccg tcgccgagtt cgccctccac
     5101 ggcttccgcg gcgccaccgt cgacgccatc gcccgccgcg cccgcctcaa caaggcgatg
     5161 atctactacc acttcgacga caagctcgcc ctctacctcg agatcctccg cggcgtcttc
     5221 accgccgttc gcgacagctc cgccgccatc gtcgcctcgc cggtcgcccc gccgcagaag
     5281 atcgccgcct tcatcgatgc cttcgaggcc gaggccgaca agcgccccta cctcccgcgc
     5341 ctgatgatgc tcgagatcgc cgacggcgcc cgccacctcg acccgcccac cctccgcgtc
     5401 atgctcggca tcttccagaa cctgcgcgcc atcctcgagg aggggcgcgc cgccggcgtc
     5461 ttccggccgg tcaaccccgt cgtcacctac ttcagcatca tcggtcccat gatgttcttc
     5521 cgcgccgccg cccccgtgcg cgagctggtc agccgcctcg acatcgagga gagcttccgc
     5581 atggacacgg ctgcctttgc cgaccacctc aaggtggcgg cgctgcagac cctggctcct
     5641 tccgtgggcg cgccggccaa cgccgcgaag ccgcgccccg cccgggcgcg cgggaagggc
     5701 cgcgccgcca agaccggaga ccgcacatga gaacgcacgc actggccgtc accctcgcgc
     5761 tggcctcgac cttcaccgtt gcctgccgct cgaacgagcc cgccggctcg gtcagggctt
     5821 ccggaaccgt cgaggccacc gaggtccagg tcgccggact ggtcgggggg cgcctgctcg
     5881 agctgaaggt cgcagagggc gaccgggtcg aggccggcca ggtggtcgca cagctcgacg
     5941 tcgccgacgc ccggctcgcg ctccagcgcg cgcaggccga gcgcgcgcag gccgacgccc
     6001 agctgcgcct gctgctcgcc ggagcgcggc ccgaggagat ccggcaggcc gaggcgcagc
     6061 ttcgcgcggc cgaggccgaa gcggccgctg cccgcgagga tctgcgaggc gccaccgccg
     6121 acctccagcg gttcgacacg ctgctcgcga acaactccgg ctcgcggaag cagcgcgacg
     6181 acgcggccac cagggtggac gtgatggggg ccagggtgcg cgcggccgag cagcggagcc
     6241 aggcggcagc cgagacgctg gcgcggttga agaagggggc gcgcgcgcag gagatcgacg
     6301 cggcccgggc gcgcctcggc gtcgtggacg cgcagattgc gacgctcgag aagagcatcg
     6361 ccgacgccac gatcgccgcg ccggtagccg gcatcgtcac gcagaaactg gcggacgtgg
     6421 gcgagatcct ggcgccgcgc gcgccggtcg tctcgatcgt ggacctcgac cacgcgtggg
     6481 cgaacatcta catcgacgag ccgctcgtgc cgcgcgtgcg gctcgggcag caggccaccc
     6541 tctacaccga cgcagggggc cccggcatcg aggggaaggt taccttcgtg tcgccgcgcg
     6601 cggagttcac cccccgcaac gtgcagaccg ccgaggagcg ctcgaagctc gtctaccgga
     6661 tcaaggtgtc ggtggacaac cgggagggaa tcctcaagca agggatgccg gtcgaggcgg
     6721 aagtgccgct gagctagggg ctaggggcta ggagctaggg gctaggggct aggggctagg
     6781 ggctgggggc taggggctag gggctggggg ctggggcgac cacaagggtc gccccgacgg
     6841 cacgagacct ggacgccggc cgacagggag ccttgggccg tcacccggcc gacgccggcg
     6901 ctactggagg ccggacgtgt tcgcgcggaa catgcctcgc ggcgcgggcg cgggcagcgc
     6961 gacgatggtg gcgccctcga cctggaccgt gtcgcccacc gcgagcggcg gcgcctgctg
     7021 gatcgcctcg aagttcacca gcgccgactc gtaggcgagc atcgtctcga gcaggttcac
     7081 ctgcgcctgc agcaggtcgc gctgcgcctg cgtcaccagg aacgtggtcg agagcccggc
     7141 ctggtagcgg cgctgctcgg cgttgagccg ctcctcggca agccgcgccc cggcgcgggc
     7201 ggcgtcgatg cgctcggcgg tgctgcgcac ctggcgtccc gcccgccgca cggcctcggc
     7261 cacgcgcagc cgcaagctgg cgatgcgctg ctcggcctgc ttgcgctcga tctcggcgcg
     7321 caccctcccc gcttcctcgt agctctggcc cagcgggtag ttcacggtca ggccgacgct
     7381 ccacgcgggg tagtcgctgc ggaacacctg gctgagcgcc tcgccgagcc cgagcgagcg
     7441 ccggccgacg atcgtgccgg ggaagccgcc gctgcgcagg aactgcgtgc cggccaggcc
     7501 gttgccgcgc cacgacgttt cgagccggac gtcgggcagc cgctggttcc cgtagaactc
     7561 caccgtcgtc ctggcgtttt cgagctggtg gcccgcccgg gcgatgtcgt accgctcgcc
     7621 gagcgcgcgg gccacggcgg catccacgtc gggctgctcc ctgagcggcg tcggctgctc
     7681 gaccgcctgc aggtgcatct gccagaacga ggcgtccgac gggtcggcga tcagcagccg
     7741 cagcgcgtcc tcggcgtcgc ccgcggtggc gtgggcgcga atcaggttct cgcggcgctg
     7801 cgcaacctcg gcctcggcct gcgtgaggtc gagcggcggc gtctgccccg cgtcgacccg
     7861 gatcctggtc tggcgggcca gctcctcggc cagttccagc gagcgctgcg ccacagagat
     7921 gttcgcctgc gtcgccctga gggtccagta cgcctgcttg acggccgcga ccgtttgcgt
     7981 caccgactcg cggaagcgaa gttccgagct ctcgaggttc cgcttcgcga tcgcgtactg
     8041 gtagcgcgca cggtccacgg agcggtccct gagcagcggc tgcgaaaacg cgatctggaa
     8101 gcccgactcg aggctgggat cgaaattgct gatggggctg ttggtcgagg tccgggccat
     8161 ctcccacgac acctgccagg taccggatcc ccacggcagc cgctggcgca ctccaaagct
     8221 ggagaacagg tcgtccacgt ccacgccgct gtcgcccgcg aggaagctgg ccggcggcgt
     8281 ggcggtgctc gagcgcccga cgctggccga gaacagcggg gtgaacgcgg ttcggctctc
     8341 gccgacgcgc gcggcgcccg cctgggtgtc gagccggacg atcgcaagct cggggttgtg
     8401 ctcgacggcg cggcgcaccg cctcctcgag cgtgagcggc aggatccgct ctgagggctc
     8461 gggttcgcgc acctgcgcgg cggcagcggc gggggacacg gcgagggcga ggaccacgat
     8521 ggaggcggcc ctcgcccggc ggctgatcgc aatcatgtgg ggagcccctt tcagacggcc
     8581 tcggccaggc ccgtaccggt atcggtcacc gggcgctcga cggcggtcgc ggtgcgccgg
     8641 cgggcgacca ggaggtagat cgccggcacg acgaagagcg tgaatgccgt gccgatcacc
     8701 atgccggtca ccagcatcat gccgatgctg ttgcgcgctc cggctcccgg gcccgtggcg
     8761 aggacgagcg ggaagtggcc ggcgacggtg gccgcggtgg tcatcaggat cggccgaagc
     8821 cgggtgcccg acgcctcgat caccgccgcc accttctggc ggccctgctc ctgcaggtgg
     8881 ttggcgaact gcacgatcag gatgccgttc ttcgacacca gcccgacgag cgtgatcagc
     8941 ccgacctggc tgtagatgtt caggctcgtc aagcccagga acgagaacag cagcgcgccc
     9001 gacaccgcca gcggcaccga gccggccagg atgatgaacg ggtcgcggaa gctctcgaac
     9061 tgcgcggcga gcaccaggta gatcaggatc gcggagagca ggaaggtggc gaggaacgcg
     9121 cgcccctcgg tccgcagctg ccgcgactcg cccgcgtagt cgatcgacac gccggccgga
     9181 agcgccgccc tggcctcgcg ctcgaggaac ccgagcgcct ggtcgagcgc caccccgggc
     9241 ggcacgaccc cctggacgcg gacggcgttg agctgctgga acttcttcag ctcgcgcggc
     9301 tcgaccgtct gccggagggt ggcgaaggtc gagagcggca ccaggcggtc gttcgagccg
     9361 gtcacgtaga tctggcgcag ctggtcgggg gtgaggcgct cggcccgctc gatctggggg
     9421 atcaccttgt agctgcgccc ctggatgctg aagcggttga cgtaatcgcc gcccagcatc
     9481 accgacaggt cgcggcccgc ctgcccgagg tccacgccct gcgaccgcag cttgtcgcgg
     9541 tcgaacacca cctcggcttg cggctggtcg aacttcacgt cggagtcggc gaagaggaac
     9601 aagccgctcg cgaacgcctt ctgcaccagc tggcccgcga tctcggccac ctgctgcggc
     9661 tcggcggggg aggcgatcac caggtccacg gggaagtcgc ctccgcccgg cagcggcggc
     9721 ggcgtcagcg ggatcgcgcg gatgccgggg atcttcgaga acggcccggc gacggccacc
     9781 tgcagctcct gcgcggtgcg ggtgcgctcg ctccagggct tggtcaccat gcccccgaac
     9841 ccgccgctcg gcagcgcgat ctggaacgtg ccggccgcct cgggaaccga ccggaagacc
     9901 cggtcgatct cgccggcgaa caggctcgtc tgctcgatcg tcgcattggg cgcggcctgg
     9961 atgatcgtga acaccacccc ctggtcctcc gagggcgcca gctcacgctg ggagaacatg
    10021 tagaacggca cgatcaggga ggcgacgatc acccagagca ccagcaccac ggggcgatag
    10081 gcgagggtcg cgcccagcgc cctcgtgtac cagccgcgca cgacgtcgaa gtggcggttg
    10141 atcatccccg cgaagccgcg ctcggtcgcc ccggcggtga ggagcttcgc gcccatcatg
    10201 ggcgacaggg tgagggcaac cactcccgag atgacgaccg caccggcgag cgtgaacgcg
    10261 aactcccgga acagcgcgcc cgtcagccct ccctggatgc cgaccggggc gtacacggcg
    10321 gccagcgtga tggtcatcgc gatgatcggc ccgaccagct cgtgcgccgc gtccatggcc
    10381 gcccgcaccg gccgcttccc ctggtgcagg tggcgctcga tgttctcgac catcacgatc
    10441 gcgtcgtcca cgacgaggcc gaccgacagg acgatcgcga gcagcgtcag caggttgatg
    10501 gtgaagccgg caaccagcat caggaacacg gcgccgacca gcgacagcgg gatcgcgacc
    10561 accgggatca gcaccgaccg caccgacccc aggaacagga agatcacgac gacgacgatg
    10621 aggagcgtct cgctgagcgt cgtgagcacc tcgccgatcg cgtcgctgat gtactcggtc
    10681 gagtcgtacg ggatgccgac cttcatcccc acgggcagct cggcccgcag ggcatcgacc
    10741 gtgtcgcgca cgcgggcgat cacctcgagc gagttcgcgg tcgggagcac ccacacgccc
    10801 atgaaggtgg ccgtctcgcc gttgaagcgc acgttctggt cgtagttctc ggcgccgagc
    10861 acgacgtcgg cgatgtctcc gaggcggacg acggccccgt cggcctcctt caccaccagt
    10921 tgccggaact cctccggcgt ccgcaggtcg gtgttcgcca cgaggttcac cgacaccatc
    10981 gaccccttgg tgcggccgag cgccgacagg tagttgttgc gcgacagcgc ctcctgcacc
    11041 gccgagggcg agatgccatt ggcggccatc cggtcgggct tgagccagat ccgcatggcg
    11101 aaggtgcggt cgcccaggat gtcggcgcgc tgcacgccgc tcaccgcgct gatcttcggc
    11161 tgcacgaccc gccgcaggta gtcggtgatc tgggtctggt cgaggtcggg cgacgagaac
    11221 ccgaggtaca tggccgcgaa ctgggtgtcc gcggtctcga gctcgatgat cggcgcctcg
    11281 gcctcgggag gcaggtcgtt gcgcacctgc gccaccttcg cctggatctg ggtcagcgcc
    11341 gcgttggtgt cgtagttgag cttcaggtga acggtgatcg tgctcaggcc ctgggcgctc
    11401 gacgactcca tgtagtcgat gccgtcggcg ctggcgatca cgcgctcgag cggcgtcgtg
    11461 atgaacccgc gcaccaggtc ggcgcccgcg ccgatgtacg ccgtcgagac gcggatcacc
    11521 gcgatgtcgc tgcgcgggta ctgcctcacg ctgagcgccc ggatcgactg cagccccgcg
    11581 atcaggatga cgagattcac cacgatcgcg agcaccgggc gcttgacgaa caggtcggtg
    11641 aacttcatca gctgttccct ggctggggcg cggggctgtt cgccggccgc acgctgttgt
    11701 cgacctcgac ggcggcgccg ttgcggagct tgaagacgcc cgacgtcacg acctcgtcgc
    11761 ccgggttcac gcccgacacg acggcgacct ggtcgccccg cgccggcccg agcttcacga
    11821 actgctgccg cacgccgcgg tagcggcggc cctgctcgtc ggtcagctcg gcgacgacga
    11881 acaccgagtc gccgtacggg gcgtagctga tcgccgaggc gggcagcgcc ttgacggggt
    11941 cctccctgcc caggaacagc tcggcctgca cgaacatccc gggccgcagc gcgcgctcgg
    12001 gattcccgag cgtggcctgc acctcgatgt tccgggtcgc ctcgtccacc accgagtcga
    12061 tggccgtcac gcgccccgcc agctcgattg cgggggagcc gtccgtcctg acgcgcaccg
    12121 tgcggcccac gcgcacctgc gtggccgcct gctgcggcac cccgaagttc acgtagatcg
    12181 ggttgagcgc ctgcagggtc accagcgggt cgccgccggc gagatactgg ccgaggttca
    12241 cctcgcgtat gccgagaatc ccggagaacg gcgcgcggat ggtcttccgc tggatcgccg
    12301 cccggatctc gccggcctcc gcgtcggtcc ggcgatactc ggccgtcgcc tggtcgaact
    12361 ccgccttgga gatgacccgc tcgttcagca gcccctgcat gcgctcgaag ttcgtccgcg
    12421 ccagctcgcg ccgcgcctcg accgccgcga gctgcgcctg ctcctggcgg gtgtcgagca
    12481 cggccagcac ctgcccctcc cgcaccgcct ggcccgactc gaactcgatg cgctccacgg
    12541 tgcccggcag atcggcgctc accgtcacgc cctgtacagc ggccaccgtc ccgatggcag
    12601 tgagggtggc cggccaccgc tcctcggaga cgacgaccgt cgtcaccgcc tctggcggcg
    12661 gctggaacga cgcggcctcg ccaatcgcgg tctggatctg cctgaacttc acgaagccga
    12721 gcgcgccgac gaagaccgcc gtcacgatca gcatcaggat catccgcttg gccatacgtc
    12781 ttccctcttc tcccttgccc cgactcccac tgtgccgcca ccccgcccgc tcgacaatcg
    12841 ggcaggagtc acaacggccc ctcggtcgaa cggacgatgc cgcgcgcgcg acggttccta
    12901 cggcaggggt tcgacgcgat tcggttcgag tgcaggcttc gacgggatgc acaaggtctg
    12961 ccgcgcggcg cgcgcacgga gcccgttagc ccgtccgcaa gtcgtgggat cgtgcggcgc
    13021 gtcccggcgc gataatcgct ggatgggacc ccagttcagt agctcgaccg agcagggccg
    13081 tacgccctgc gggcggccgc tgcagccccc ggatcccgag ggcgcctcct cgaacgaccc
    13141 cgaggtgctg cgcgccgcgc tgcttcgcga ggccgcggag cgccgccgcg ccgaggtgct
    13201 ggcgaagatc cagaccgagg tggcccgggt gacgctcgac ctcctggtgc gcgagcccga
    13261 catcgagggc ttcttcggcg cgctgaccca gaccatggtc gagaagggag gcaaccacgg
    13321 ctgcggcgtg tggctgatcg acgacgaccg gcaggactgc cggctctgga tggcctgcct
    13381 cgacggccag ctcgtgaccc cgcgaagcgc gggctgggac gcgatggcgc tgccgcgcga
    13441 cagcatgtcg gcgcacctct tcgcctattc cccggggtgg cagcagacgg tggagtacgc
    13501 cggcggcgac acccgcctgc ccgagccggt gcgcgagttc caccgccgcg agggaatcga
    13561 caaggtcatc gtcacgccgt tgatcctcgg cagccacaac ctggggtgga ttgcgatcac
    13621 cgcctacgac atctccgggt gcgacaagtg gtggcacgtc gcgctggtcg aggcggtcgc
    13681 ccgccaggcg accctcgccc tctaccagag ccggctcgcc gagcgcacgc agctcgagga
    13741 gcggcgcaag gcggttctcg aggagcgcaa ccggctggcg cgcgacatcc acgacaacct
    13801 cgcccagggg ttcgccgcga tcctgatgca gctgcaggcg gcgctgcgcg gggcgccgcg
    13861 cctcgaggcc gggctggcgc gcaagctcga gaccgcggtc gagctggcgc gcacgcacat
    13921 gatcgaggcg cgccggtcag tcggcacgct gcgccccaac gtgggcgacg gcgaggacat
    13981 cggccaggcg ctcgcgcgca tcgcggcgct cgcccagcgc accaccggca cgccgatcga
    14041 cgtcgccctg gacgacctgc cgcgcttcgg cgacgtggtc gagcgcgaga tcgtcgcgat
    14101 cgtgcaggag gccctcaaca acgcggtgcg ccactcgcgg gccgggcgca tccgcgtcga
    14161 ggccgggccg gtgcgttcga tcgggctccg ggtctcggtt tccgacgacg gccgcggcat
    14221 cccacgcgag tcgtcgggct cggggttcgg cttgaccagc atgcaggagc gcgccgagcg
    14281 gatcggcgcc tcgttgacca tcgtcacggc gccacggagc gggacggaag tcgtcctggc
    14341 gtgggagccg tcttcgcttc ccacacaggt gcatgtcgcg ggatgaattc gacgccggcg
    14401 tggccgtggc caaggccagc gtgctggtcg tggacg
//
LOCUS       DAOPQX010000054        14376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4931_length_14376_cov_4.661336, whole genome shotgun sequence.
ACCESSION   DAOPQX010000054 DAOPQX010000000
VERSION     DAOPQX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14376
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_14376_cov_4.661336"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKK95_04730"
     CDS             complement(<1..373)
                     /locus_tag="PKK95_04730"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOC17549.1"
                     /translation="MIDTRPALGELMAAFDALAGGGWQVRTIASQVAPDGGLLPIRAY
                     FNADEVNLVLVAGIHGREPAGPVALARLVPSLIANGLNRRLLVMPLLNPWGYVQCERY
                     GPSGKSVSDSDHLLGRAAAPAC"
     gene            complement(411..1742)
                     /locus_tag="PKK95_04735"
     CDS             complement(411..1742)
                     /locus_tag="PKK95_04735"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HOC17550.1"
                     /translation="MQTSHIRTPLPGPNAQALIARDALVTSPSYPRDYGFAMSHGRGC
                     EVWDVDGNRFLDFAAGIAVCSTGHCHPEVVAAIGRAAEKFLHISSDFWHEGQTRLAER
                     VSALAPMRERAMCFFGQSGTEAVEAALKLARYATGRSRFIGFLGGFHGRTMGSLAFTS
                     SKYTQQRRFAPAMPGVTHVPYPNTYRPLFAGDDQGQAVVDYIEQAIFRGNVPPDEVAA
                     ILVEPMQGEGGYLVPPDGFLSRLRELCDRHGILLIFDEVQTGIGRTGRMFACEHWGVQ
                     PDIMTLAKGLGSGMPIGAMVARHGVMEAWPRGAHGNTFGGNPLCCAAALATLDLVERE
                     YMANAARVGEQFLARLRDLQARHPEIGDVRGKGLFLAIELVKDRASKEPAHGFCEAVV
                     RQAFQNGLLLLSCGVSTIRFMPPLMVTGPEVDEALAILEASLNEARRGAGV"
     gene            complement(1807..2595)
                     /locus_tag="PKK95_04740"
     CDS             complement(1807..2595)
                     /locus_tag="PKK95_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HOC17551.1"
                     /translation="MKVPWTPDEMMTVAAARLLWDGCVCFVGIGLPSAAANLARQTHA
                     PEIVLIYESGTVGTHPHVLPLSIGDGELAETATAVVPLPEIFAYWLQGGRIDVGFLGA
                     AQIDRFANLNSTVIGDYARPKTRLPGAGGAPEIAANARQIFIVLKQTPRAFVPRLDFC
                     SSAGFMDGRGARARSGAPGKGPQVVITDLGVLRPEPGSEELRLVGLYEGATVDQAVKA
                     TGWPLRVADTLEVVAPPTHEELQVLRDLHARTKDAHSRPVSVPY"
     gene            complement(2592..3473)
                     /locus_tag="PKK95_04745"
     CDS             complement(2592..3473)
                     /locus_tag="PKK95_04745"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOC17552.1"
                     /translation="MKLTTLHDAIAAHVRDGQTIAMEGFTHLIPFAAAHEIIRQGRKD
                     LHLVRMTPDIVYDQMIGAGCARALTFSWGGNPGVGSLHRLRDAVEKGWPRPIEIVEHS
                     HAGMAASYTAGASNLPFGVLRGYIGTDLPAVNPQVRSVTCPYSGEKLATVPALRPDLT
                     ILHAQLADRSGNVLLRGILGAQKEAAMAARRLVVTVERVVDELDASMNAVVLPSWIVT
                     AVACVPGGAYPSYAQGFYARDNAFYLAWDEIARDRDRFRGWMDEHVLGTRTHAEFLRG
                     LGVPAAEGGPSAAEVIQ"
     gene            complement(3478..5049)
                     /locus_tag="PKK95_04750"
     CDS             complement(3478..5049)
                     /locus_tag="PKK95_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOC17553.1"
                     /translation="MTAFKLTYSTMFDPPEEMHTSFEAALAEVRKGLGASHAMFIDGG
                     DAEGIRTVSTYSPVDERVELGRFAAGDASHVDQAVAAARRAFDPWRRTPARERVELVR
                     RVAALLEERVYSIGAALALEVGKNRMESLGEAQETADFFWGYAADFERNHFYDRPLPD
                     DPLEGWKSHNRSVLKPHGVWAVIAPFNYPLALAGGPAAAALVTGNTVVLKGGSDTPWA
                     GRLLAGCIRDAGMPPGVFNYVIGSGPVVGERLIHHPDLAGVTFTGSYDVGMGIFRAMA
                     AGRWPRPCIVELGGKNPCIVTARGDLDRAATGIVRSAYGLSGQKCSALSRVYVEEAVA
                     DALIERLVPKVAALAVGDPTRREIYMGPVISRRAAASFDDCCRRLEGGGARILAGGTR
                     LAGGELEHGHFVAPTLAEAPAEHPLFQEEMFLPILMVNRVRSLDEALERTNGATLGLT
                     AGCYGNEDEVAAFLDRIEAGVTYLNRPQGATTGAWPGYQAFGGWKGSGSTGKAIGSFY
                     YLPQYLREQSQTWVE"
     gene            complement(5046..6353)
                     /locus_tag="PKK95_04755"
     CDS             complement(5046..6353)
                     /locus_tag="PKK95_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC17554.1"
                     /translation="MALERDLVLHSWCVQADWNAPTIVGGRGARFFDASGRSFLDMSS
                     LAECANLGHQHPTVVRAIQEQAERLCFVTAAWGAEPRATLAALLLERSGFDGGRVFFT
                     LGGADANEQAVKFARQASGRPQGWVIARDRSYHGASYGAMAFSGDSRTRRQVDPAAFH
                     VRHVPPPYAYRCPFGSISPAECGERAAASVASALDAHPDAAAVLMEPDAGTNGIVAPD
                     TFWPALRKVTRERNVWLIADEVMSGFGRCGEWFAWQRYGESGRPDLMTLAKGLTGAHL
                     PLAAVVLSREVASLLEHEMLYTGLTYCGHPLACAAGVAAVRAYEDERLIERSRTLGGW
                     LFQELLALQRRHEVIGDVRGGHGLFAVLELVKDRATREPLRPWPEVHPSLKRLLVRGL
                     EAGVSFAARGNLVILAPPLVIGERELADALDLLDRLLGDTEWS"
     gene            6480..7682
                     /gene="pcaF"
                     /locus_tag="PKK95_04760"
     CDS             6480..7682
                     /gene="pcaF"
                     /locus_tag="PKK95_04760"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF006551.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOC17555.1"
                     /translation="MRPVFLCAVVRTPFGRHGGALARVRTDDLAAFPIRTLLERLPHV
                     DWAALDEVILGCANQAGEDNRNVARMASLLAGLPPEVPGVTVNRLCASGLEAVGQGAR
                     AIRCGEADLVIAGGVESMSRAPLVVAKAEAAFSKDQRMEDTTVGWRFVNPRMQERYGT
                     DGNPQTAENLARERGISRDDQDAYALRSQQRAEAARQRGLLADEIAPIDVPAGRETKR
                     VSEDEHPRPGTSLEALAKLKPLLSAGTTITAGNASGVNDGACALLLASGPAIERHGLE
                     PLARVGGMASAGVPPCIMGIGPVPAIRRLLEREHLALADVDVIEINEAFAAQVLACTR
                     ALGLRDDAEFVNPNGGAIAFGHPLGASGARLCLTAALELRRRGGRRAIVSLCVGVGQG
                     LAMLLERP"
     gene            7690..9036
                     /locus_tag="PKK95_04765"
     CDS             7690..9036
                     /locus_tag="PKK95_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC17556.1"
                     /translation="MTAADPPARASRIGFWMATALVVGNMIGSGIFLLPAALAPFGAD
                     NLPGWLLTAGGAVTLALVFAHLSRVVPGAGGPYAYTREAFGDLAGFTVGWAYWVSVWV
                     GNAAIATGAVGYLGSFVPWMVASPLNTALATLGFVWAFTFVNCAGIRAAGWVQGITTV
                     LKLLPLVAAIALLVSRLDTVDWHGAGTATFTLGGTVSVVTLTLWAMLGLESATIPADK
                     VEGASRTVFRATMWGTVLTIVISAAACSAVLLLVPASRAASSSAPFADLATSAWGAGA
                     ARAVSLFAAISALGALNGWTLLQGELPRAMAQDGLFLRCFAKTSARGAPVVALLATSG
                     VVTALVLLTVSRSLVGIFTFFVLLATTATLVAYLACALALLRLRARGRAGAPQLGLAG
                     LGLLACVGAAYSIGALIGAGREAVLWGAVLLAAGLPVYALLRRQARERIAAREAGL"
     gene            9254..10582
                     /locus_tag="PKK95_04770"
     CDS             9254..10582
                     /locus_tag="PKK95_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOC17557.1"
                     /translation="MERPHPPDFIDTRIVLRVYAALCLLAGVALLALSAGGGFRGPSQ
                     AVAGRAAVLLAAACAAMGLARVESPRDRHDALAWFANGHLVLLAALLAMSLLRGGPRT
                     SEVYLPFLAAATMLLYYFWQTGDGHRARESAESITLFPHGPRGNEHLRSEYEERIREA
                     AAQEERHRLARELHDSVKQQIFAIQTAAATVQARYDADPPGASEALGRLRAYAREAMT
                     EMEAMLDSLRASPLESAGLVEAIKRQAEALRFRTGAEVAVEIGELPRADEMPPGVAQG
                     VFRVAQEAFANVARHARARAVKVRLLVQGNALVLSVADDGAGFEPAAATAAGMGLTGM
                     GSRAEALNGRLTVTTRPGAGTTVTIRVPLGPRARPDISDYRRRAVTWGACSLLQGLLV
                     AWRVATGQPVGYAVAVLAVFVVVTGRACIGWRRARRSAESSRWIQSPSHS"
     gene            10555..11193
                     /locus_tag="PKK95_04775"
     CDS             10555..11193
                     /locus_tag="PKK95_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC17558.1"
                     /translation="MDPVSVALVDDHRVVGISLKAYLESFPDILVVGVASSGEELLAR
                     LDQWHPAVVLQDLLLPGGIDGVETTRRILRQSPSTRVIVLTASMDEARMVGALRAGAA
                     GYVRKDAQPEALLDAIRAVARGKTYIDPSVARRVIVGAGPRDDLTGREVEVLRAIGLG
                     QTNKQIAEQLGVTEETVKTHVASLLAKLEVDNRTQAVVEAIRRGIVGVEELE"
     gene            11247..12074
                     /locus_tag="PKK95_04780"
     CDS             11247..12074
                     /locus_tag="PKK95_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOC17559.1"
                     /translation="MPHHASIHALVADLASLGVATGDLVMVHASLRRLGPVERGAEGV
                     IEALDEAVGPGGTVMMVLGARDDWAWVNDRPEPERAALLEGSPPFDALATPASPEVGV
                     LAEVFRRTPGTRVSDHPEGRFGARGLLARHLLLDVPWDDYYGPGSPLERLVDGGGKVL
                     RLGADPNTVTLIHYAEYLAEVPGKRRVRRHRLVATPRGPELRIVECLDDNNGIVDYPG
                     GDYFADILQAYLEQGRARQGLVGQAGSELLDARDLAEFAARWMTKRFGSGGATPPAG"
     gene            12207..13532
                     /gene="xylA"
                     /locus_tag="PKK95_04785"
     CDS             12207..13532
                     /gene="xylA"
                     /locus_tag="PKK95_04785"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF003998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOC17560.1"
                     /translation="MSHFIGEKEFFPGIPAIKYEGRDSTNPLSFKFYDRDKKVLGKTM
                     GEHLRFAVAYWHSFCAEGSDQFGDGTRVFPWGSAPTPMDRARQKLDAAFEFITKLGAA
                     FYCFHDRDIAPAGATVAESEKNLQTLVALAKERQQATGVQLLWGTANLFSHPRYMNGA
                     ATNPDFRVVTHAAAQVKAAIDATIALGGQGYVFWGGREGYTTLLNTDMKRELENLGRF
                     LSMARDYGRRQGFTGTFYIEPKPMEPTKHQYDFDAATVIGFLRAHGLEKDFKLNIEAN
                     HATLAGHSFSHELQVAADAGLLGSIDANRGDPQNGWDTDQFPTDLYQATEAMMIVLRA
                     GGFTTGGLNFDAHIRRNSTDPEDLFLAHIGGMDTFARALEIAARLLNESPLKDLTKKR
                     YASFDEGNGLRFTKGELTLEDLARLAGQGEPPLVSGKQELIENVINQYL"
     gene            complement(13671..>14376)
                     /locus_tag="PKK95_04790"
     CDS             complement(13671..>14376)
                     /locus_tag="PKK95_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC17561.1"
                     /translation="ARLIGRLVEAHAQFPFDFVIMLGDNLYGSERPEDYRRKFEIPYK
                     ELLDAGVRFYAALGNHDEPEQRLYGPFNMDGERYYSFTRGPVQFFVLDTTDLDAKQVD
                     WLRRELAASQARWKIAYFHHPLYSSGGKHGSDTTLRAILEPLFVEHGVSAVFAGHEHF
                     YERIKPQKGITYFTSGGAGKLRRGDIQDTALTAAGFDRDRSFMLVEIAGDTMHFQAIS
                     RTGETVDSGTLPVRAR"
BASE COUNT         2048 a         5084 c         5141 g         2103 t
ORIGIN      
        1 ggcaggccgg cgccgccgca cgcccgagca ggtgatcgct gtccgagacg ctcttgccgg
       61 aggggccgta ccgctcgcac tgcacgtagc cccacgggtt gaggagcggc atgaccagca
      121 ggcggcggtt gaggccgttc gcgatcaggg agggcaccag gcgggccagg gccaccgggc
      181 ccgccggttc gcgaccgtgg atgccggcaa ccaggacgag gttcacctcg tcggcgttga
      241 aataggcgcg gatgggcagc agcccaccgt cgggtgccac ctggctggca atcgtgcgca
      301 cctgccaccc gccaccggcg agcgcgtcga aggcggccat cagctcgccg agtgccgggc
      361 gcgtgtcgat catgtcgcct tccgtagggg cgggccttgt gcccgcccgg ttacactccc
      421 gcgcccctcc gcgcctcgtt caagcttgcc tcgaggatcg cgagcgcctc gtccacctcg
      481 ggcccggtca ccatcagcgg cggcatgaac cggatggtgc tcacgccgca cgacagcagc
      541 agcaacccgt tctggaacgc ctgccgcacg accgcctcgc agaacccgtg cgccggctcc
      601 ttcgatgcgc ggtctttcac cagctcgatc gccaggaaca gccccttgcc gcgaacgtcc
      661 ccgatctccg ggtgccgcgc ctgcaggtcg cgcagcctcg cgaggaactg ctctccgacc
      721 cttgccgcgt tcgccatgta ctcgcgctcg acgagatcga gcgtcgcgag cgccgcggcg
      781 cagcacagcg ggttgccgcc gaacgtgttc ccgtgggctc cacggggcca cgcctccatc
      841 acgccgtggc gcgccaccat ggccccgatc ggcataccgc tgccgagtcc cttcgcgagc
      901 gtcatgatgt cgggctgcac gccccagtgc tcgcaggcga acatcctccc cgtccggccg
      961 atgccggtct gcacctcgtc gaaaatgagc aggatgccgt ggcggtcgca cagctcgcgc
     1021 agccgggaga ggaagccgtc cggcggcacc aggtaccctc cttcgccctg catcggctcg
     1081 accaggatgg ccgccacctc gtcgggcggc acgttcccgc ggaagatcgc ctgctcgatg
     1141 taatcgacga cggcctggcc ctgatcgtcg ccggcgaaca gcggccgata cgtgttcggg
     1201 tacggcacgt gcgtcacgcc gggcatcgcc ggcgcgaacc gccgctgctg cgtgtacttg
     1261 ctcgacgtga aggcgagcga gcccatcgtc cggccgtgga agccgcccag gaagccgatg
     1321 aagcggctgc gccccgtcgc gtagcgcgcc agcttcagcg ccgcctccac cgcctcggtg
     1381 cccgactggc cgaagaagca catcgcccgc tcgcgcatcg gggcgagggc cgagacgcgc
     1441 tcggcaagcc gcgtctgtcc ctcgtgccag aaatcgctcg agatgtggag gaacttctcc
     1501 gccgcccggc cgatggccgc cacgacctcg gggtgacagt ggccggtgct gcagacggcg
     1561 atgccggcgg cgaagtcgag gaaccggttg ccgtccacgt cccacacctc gcagccgcgg
     1621 ccgtgcgaca tcgcgaaccc gtagtcgcgc gggtaggagg gcgaggtgac cagggcgtcg
     1681 cgggcgatca gcgcctgcgc gttcgggccg ggaaggggag tgcgaatgtg agaagtctgc
     1741 atagaaggct cccgcctcgc tccgcgtccg cagtgggaca ggcgtcccgc ctgtccggaa
     1801 acaaactcag tacggcacgc tcaccgggcg cgagtgcgcg tccttggttc gtgcgtgcag
     1861 gtcgcgcaag acctgcagtt cctcgtgggt tggcggcgcc actacctcca gcgtgtccgc
     1921 cacccgcagc ggccacccgg ttgccttgac ggcctgatcc accgtcgcgc cctcgtagag
     1981 cccgaccaga cgcagttcct cggagccggg ttccgggcgc agcacgccga ggtcggtgat
     2041 caccacctgt ggacccttgc ctggcgcccc gctacgggcc cgcgccccgc ggccgtccat
     2101 gaagcccgcc gagctgcaga agtcgagcct cggcacgaag gcccgcggcg tctgcttcag
     2161 cacgatgaag atctgccggg cgttcgccgc gatctccggg gcgccgcccg cgcccggcag
     2221 ccgggtcttc ggccgcgcgt agtcgccgat caccgtgctg ttgaggttcg cgaaccggtc
     2281 gatctgcgcc gccccgagga acccgacgtc gatgcggccg ccttgcagcc agtaggcgaa
     2341 gatttcgggg agcgggacca ccgcggttgc cgtctcggcc agctcgccgt cgccgatcga
     2401 gagcggcagg acgtgcgggt gcgtccccac cgtccccgac tcgtaaatca gcacgatttc
     2461 cggcgcgtgc gtctggcggg ccaggttcgc agccgccgac ggcagaccga ttcccacgaa
     2521 gcagacgcaa ccgtcccaca gcagccgcgc ggcagccacc gtcatcatct cgtcgggcgt
     2581 ccacggtacc ttcactggat cacctccgcc gcgctcggcc cgccctccgc cgccggcacg
     2641 ccgagccccc gcaggaactc tgcgtgcgtg cgggtgccga gcacgtgctc gtccatccag
     2701 ccacggaagc gatcgcggtc gcgcgcgatc tcgtcccacg ccaggtagaa cgcgttgtcc
     2761 cgcgcgtaga acccttgcgc gtaggagggg taggccccgc caggcacaca cgccaccgcg
     2821 gtgacgatcc acgaggggag cacgacggcg ttcatcgagg cgtccagctc gtctaccacg
     2881 cgctcgacgg tgaccacgag gcgccgggcg gccatcgcgg cttccttctg cgcccccagg
     2941 atgccccgca gaaggacgtt tccggaacgg tcggccagct gcgcgtggag aatggtgagg
     3001 tcgggccgca gcgccggcac cgtcgccagc ttttccccgc tgtagggaca ggtcaccgac
     3061 ctcacctgcg ggttcaccgc cggcaggtcg gtgccgatgt agccccgcag cacgccgaac
     3121 ggcaggttcg acgccccggc ggtgtaggag gccgccatgc ccgcgtgcga gtgctcgacg
     3181 atctcgatcg gccggggcca tcccttctcg accgcgtcgc gcaggcggtg cagcgacccg
     3241 acgccgggat tgccgcccca cgagaaggtg agcgcccgcg cgcagcctgc gccgatcatc
     3301 tggtcgtaga cgatgtcggg cgtcatgcgg accaggtgaa ggtccttgcg gccctgccgg
     3361 atgatctcgt gcgccgccgc gaaggggatg aggtgggtga atccctccat cgcgatggtc
     3421 tggccgtcgc ggacgtgcgc cgcaatggcg tcgtggagag tggtcagctt catcgggcta
     3481 ctccacccac gtttgcgact gctcccgaag gtactgcggc aggtagtaga acgagccgat
     3541 cgccttgccg gtggagcccg atcccttcca gccgccgaac gcctggtacc cgggccaggc
     3601 cccggttgtc gcgccctgcg gccgattcag gtaggtgacc ccagcttcga tccgatcgag
     3661 gaacgcggcc acctcgtcct cgttcccgta acaccccgcc gtgaggccga gcgtggcccc
     3721 gttggttcgc tccaaggcct cgtcgagact cctcacgcgg ttcaccatca ggatcggcag
     3781 gaacatctcc tcctggaaca gcggatgctc ggcgggcgcc tcggccagcg tcggcgcgac
     3841 gaagtggccg tgctccagct ctccgccggc gagccgggtg ccgccggcca ggatccgcgc
     3901 gccgcctccc tcgagccgcc ggcagcagtc gtcgaagctg gcggctgcgc gccgcgagat
     3961 gaccggcccc atgtagatct cgcgccgcgt gggatcgccg acggccagcg cggcaacctt
     4021 cgggacgagc cgctcgatca gagcgtctgc aaccgcctcc tcgacgtaga cccgcgagag
     4081 cgccgagcac ttctgcccgc tgagcccgta agccgaccgc acgatccccg tcgccgcgcg
     4141 atccaggtcg ccgcgcgcgg tgacgatgca cgggttcttg ccgcccagct cgacgatgca
     4201 cggccgcggc cagcggccgg ctgccatcgc gcggaagatg cccatcccga cgtcgtacga
     4261 gccggtgaag gtgacgccgg ccaggtcggg gtggtggatg aggcgctcgc ccaccaccgg
     4321 cccgctcccg atgacgtagt tgaacacccc cggtggcatg ccggcgtcgc ggatgcaccc
     4381 ggcgagcagg cgcccggccc acggcgtgtc gctcccgccc ttcagcacga ccgtgttccc
     4441 ggtgacgagc gccgccgcgg ccggcccgcc ggccagcgcg agcgggtagt tgaacggggc
     4501 aatcaccgcc cacacgccgt gcggcttgag cacgctccgg ttgtgcgact tccacccctc
     4561 gagcgggtcg tcgggcaggg gacggtcgta gaagtggttg cgctcgaagt cggccgcgta
     4621 gccccaaaag aagtcggccg tctcctgcgc ctcgccgagc gactccatgc ggttcttgcc
     4681 gacctcgagc gccagggccg cgccgatcga gtacacacgc tcttcgagga gcgccgccac
     4741 gcgccgcacc agctcgacac gctcgcgcgc cggcgtccgc cgccaggggt cgaaggcgcg
     4801 ccgcgcagcc gcgacggcct ggtccacgtg agacgcgtct ccggccgcga accgcccgag
     4861 ctccacccgc tcgtccacgg ggctgtacgt ggagaccgtc cggatcccct cggcgtcgcc
     4921 gccgtcgatg aacatcgcgt ggctggcccc gaggcccttc cgcacctccg ccagcgccgc
     4981 ctcgaaggag gtgtgcatct cctcgggcgg gtcgaacatc gtcgagtagg tgagcttgaa
     5041 ggcggtcatg accactccgt gtcgccgagc aggcgatcga gcagatcgag cgcgtccgcc
     5101 agctcgcgct cgccgatgac gagcggcggg gcgaggatga cgaggttgcc gcgcgccgcg
     5161 aacgagacgc cggcctcgag gccgcggacg agcaggcgct tgagagaagg gtgcacctcg
     5221 ggccagggcc gcagcggctc gcgcgtcgcg cggtccttca ccagctcgag caccgcgaac
     5281 aggccgtggc cgccgcggac gtcgccgatg acctcgtgcc ggcgctgcag cgcgagcagt
     5341 tcctggaaga gccagccgcc gagcgtgcgg gagcgctcga tcagccgctc gtcctcgtac
     5401 gcgcgcaccg cggccacgcc ggcggcgcag gcgagcgggt ggccgcagta ggtgaggccc
     5461 gtgtagagca tctcgtgctc gagaagcgag gcgacctcgc gcgagagcac gaccgcggcc
     5521 agcggcaggt gggcgccggt cagccccttg gccagcgtca tcaggtccgg ccgtcccgat
     5581 tcgccatacc tctgccaggc gaaccactcg ccgcagcggc cgaagccgct catcacctcg
     5641 tcggcgatga gccagacgtt ccgctcgcgc gtgaccttgc ggagagccgg ccagaatgtg
     5701 tcgggcgcca cgatgccgtt ggtgccggcg tcgggctcca tcagcacggc ggcggcgtcg
     5761 ggatgggcgt cgagggcact cgccaccgag gctgccgcgc gctcgccgca ctctgcagga
     5821 gagatgctgc cgaagggaca gcgataggca tagggaggcg gaacgtggcg cacgtggaag
     5881 gcggcgggat cgacctgccg gcgtgtccgg ctgtcgccgc tgaacgccat cgcgccgtag
     5941 ctggccccgt gataggagcg atcgcgggcg atcacccagc cctgcggccg cccgctcgcc
     6001 tgccgcgcga acttgaccgc ctgctcgttg gcgtcggcgc cgccgagcgt gaagaagaca
     6061 cgcccgccgt cgaagcctga tctttcgagg agcagggcgg ccagcgtcgc gcgcggctcg
     6121 gcgccccagg cggcggtgac gaagcagaga cgctcggcct gctcctggat cgcccggacg
     6181 acggtcgggt gttgatggcc gaggttcgcg cactcggcca ggctgctcat gtcgaggaag
     6241 gatcgtcccg aggcgtcgaa gaagcgggcg ccgcggcccc cgacgatggt gggtgcgttc
     6301 cagtcggcct gcacgcacca gctgtgcagg accaggtccc gttcgagggc catgagggat
     6361 ccactggtgg ctgcggccgc cagcggaggc cgcaagggat gcgacgttgc ggtttcatta
     6421 gaattcctct acgctgcgct gtcaacggga gtcgggattc gggaatcggg attcgggata
     6481 tgcgccctgt gttcctttgc gcggtcgtgc gtactccgtt cgggcggcac ggcggcgccc
     6541 ttgcgcgcgt caggaccgac gacctggccg cgttccccat caggacgctg ctcgagcggc
     6601 tgccgcacgt ggactgggcc gcgctcgacg aggtgatcct ggggtgtgcc aaccaggccg
     6661 gcgaggacaa ccggaacgtc gcgcggatgg catcgttgct ggcgggcctt ccgccggaag
     6721 tgccgggcgt caccgtcaat cgcctctgtg cctcgggcct cgaggcggtc gggcaggggg
     6781 cgagggccat ccgctgcggc gaggccgacc tcgtgatcgc cgggggtgtc gagagcatgt
     6841 cgcgggcgcc gctggtcgtc gcgaaggccg aggcggcctt ctcgaaagac cagcgcatgg
     6901 aggacaccac ggtcggctgg cgcttcgtca acccgcggat gcaggaacgc tacggcaccg
     6961 acggcaaccc gcagacggcc gagaacctgg cccgcgaacg cggcatctcg cgcgacgacc
     7021 aggacgccta cgcactgcgc agccagcagc gggccgaagc ggctcggcag cggggcctcc
     7081 tcgccgatga gatcgcaccc atcgacgtgc cggcggggcg cgagacgaag cgggtgtcgg
     7141 aggacgagca cccgcgcccg ggcacctcgc tcgaggcgct tgcgaagctg aagccgctgc
     7201 tgagtgccgg gacgacgatc accgcgggca acgcgtcggg cgtcaacgac ggcgcttgcg
     7261 cgctgctcct ggcctcgggg cccgcgatag agcgccacgg cctcgagccg ctcgcgcggg
     7321 tggggggaat ggcgagcgcg ggcgtgccgc cctgcatcat gggcatcggc ccggtgcccg
     7381 ccatccgcag gctgctcgag cgcgagcacc tggcgctcgc cgacgtggac gtcatcgaga
     7441 tcaacgaggc gttcgccgcg caggtgctcg cctgcacgcg ggcgctcggc ctgcgcgacg
     7501 acgcggagtt cgtgaacccg aacggcggcg cgatcgcgtt cggccacccg ctcggcgcaa
     7561 gcggcgccag gctgtgcctc acggccgcac tcgagcttcg ccgccgcggc ggccgccgag
     7621 ccatcgtcag cctgtgtgtc ggcgtcggcc aggggctggc catgctgctc gagcgaccgt
     7681 gacaacaaca tgaccgctgc cgaccctccc gcgcgcgcct cccgcatcgg tttctggatg
     7741 gcgaccgcac tggtcgtggg caacatgatc gggtcgggga tcttcctcct gcccgccgcc
     7801 cttgccccgt tcggcgccga caacctcccc ggctggctgc tgacggccgg aggtgccgtg
     7861 acgctggcgc tggtcttcgc gcacctgagc cgcgtcgttc ccggggccgg cgggccgtac
     7921 gcctacaccc gcgaggcgtt cggcgacctc gcgggcttca cggtcgggtg ggcgtactgg
     7981 gtgtcggtct gggtgggcaa tgccgccatc gccaccggcg cggtcggcta cctcggctcg
     8041 ttcgtcccgt ggatggttgc ctcgccgctg aacacggcgc tcgcgacgct tggcttcgtc
     8101 tgggcgttca ccttcgtgaa ctgcgccggg atccgcgcgg cgggctgggt gcagggcatc
     8161 accaccgtcc tgaagctgct gccgctggtg gccgccatcg cgctgctggt gtcgcgcctc
     8221 gacacggtgg attggcacgg cgccggaacg gcgaccttca cgctgggcgg aacggtctcg
     8281 gtggtcacgc tcacgctctg ggcgatgctc ggcctcgagt cggcgaccat cccggccgac
     8341 aaggtcgagg gggcctcgag gacggtgttc cgcgcgacga tgtggggaac cgtcctgaca
     8401 atcgtgatca gcgcggccgc gtgctcggcc gtgctgctgc tcgtgccggc ctcgcgcgcc
     8461 gcctcgtcga gcgcgccgtt cgccgacctc gccacctcgg cctggggggc gggggctgcc
     8521 cgcgccgtgt cgctgttcgc cgccatcagc gcgctcggcg ccctcaacgg ctggacgctg
     8581 ctgcaggggg agctgccccg cgcgatggcg caggacgggc tgttcctgcg ctgcttcgcg
     8641 aagacctccg cgagaggcgc gccggtcgtg gcgctgcttg cgaccagcgg cgtggtgacg
     8701 gcgctggttc tcctcacggt ctcgcggtcg ctcgtcggca tcttcacctt cttcgtcctg
     8761 ctggccacca ccgccacgct cgtcgcgtac ctggcgtgcg cgctcgccct gctgcggctg
     8821 cgggcgcggg gccgcgccgg cgcgccgcag ctcggcctcg ccggtctcgg tcttctggcc
     8881 tgcgtcggcg cggcttactc gattggcgcg ctgatcggcg ccggccgcga ggccgtgctg
     8941 tggggcgcgg tgctgctggc cgccggcctg ccggtgtacg cgctgctgcg gcgacaggcc
     9001 cgggagcgaa tcgccgcgcg tgaagcggga ctgtaggccc actgctcacg agtgttgagc
     9061 aaggtcgtga gcgggtgacg cggggcggag ggcccccgct cgcctggagc cgcccgcatc
     9121 cgtgctcaat cctagagagc gccggaccgg cagggtgagg ctgcgcagcg cccccccatc
     9181 ccccgatcga gggaggccgg ttccgaccat cgggggattg gctggtgctc gcgggcggta
     9241 gactctggtg gccatggagc gaccgcatcc tcccgacttc atcgacacgc ggatcgtcct
     9301 cagggtctac gccgccctgt gcctgctcgc gggagttgcg ctcctggcac tttcggcggg
     9361 cggcggtttc cgcggtcctt cgcaggccgt tgccgggcgg gccgccgtgc tgctggctgc
     9421 agcctgcgcg gcgatgggcc ttgcccgcgt ggaaagccct cgcgaccggc acgatgcgct
     9481 cgcctggttc gcgaacggac acctcgtcct gctcgcggcg ctgctggcga tgagtctgtt
     9541 acgcggcggg cctcgcacct cggaggtgta cctccccttc ctcgccgccg ccaccatgct
     9601 gctctactac ttctggcaga cgggcgacgg tcacagggcg cgggagtcgg cggagtccat
     9661 caccctcttc ccgcacggcc cgcgcggcaa cgagcacctg cgatcggagt acgaagagcg
     9721 gattcgggag gcggctgccc aggaagagcg ccaccgcctt gcgcgcgagc ttcacgactc
     9781 ggtcaagcag cagatcttcg ccatacagac cgcggccgcc accgtgcagg cgcggtacga
     9841 cgcggaccct ccgggcgcca gcgaggccct cgggcggctg cgagcctatg cccgcgaggc
     9901 gatgaccgag atggaagcga tgctcgacag cctgcgggcg tcaccgctcg agagcgccgg
     9961 gctcgtggag gccatcaaga ggcaggccga ggcgcttcgt ttccggaccg gcgccgaggt
    10021 ggccgtcgag atcggtgagc tgccgcgcgc ggacgagatg ccgccgggcg tggcccaagg
    10081 tgtcttcagg gtggcgcaag aggccttcgc gaacgtggcg cgccacgcca gggctcgcgc
    10141 cgtgaaggta cgccttcttg tccagggcaa cgctctcgtg ctttccgtag cggacgacgg
    10201 ggccgggttc gagccggcgg cggcgacggc agccgggatg gggctgaccg ggatgggctc
    10261 cagggccgag gcgctgaacg gccggctgac ggtgacgacc cgccccggcg ccgggacgac
    10321 cgtcacgatc cgcgttccgc tcggcccgcg cgcccggccc gacatctcgg actaccgccg
    10381 ccgggccgtc acctgggggg cctgctccct gcttcaggga ctgctggtgg catggcgggt
    10441 ggcgacgggg caaccggtcg gctacgccgt ggctgtcctg gcggtcttcg tcgtggtcac
    10501 cgggcgggcg tgcatcggtt ggcggcgggc tcgccgttcc gcggagtcgt ccagatggat
    10561 ccagtctccg tcgcactcgt agacgaccac cgcgtggtgg ggatcagcct caaggcttat
    10621 ctcgagtcgt ttcccgacat cctcgtggtc ggggttgcct cgagcgggga ggagctgctg
    10681 gcccgcctcg atcagtggca tccggccgtc gtgttgcagg acctcctgct gccgggcggc
    10741 atcgacgggg tcgaaaccac gcgccgcatc ctgcgtcagt cgccgtccac gcgcgtgatc
    10801 gtgctgacgg cgtcgatgga cgaggcccgg atggtcggcg cccttcgggc cggcgccgcg
    10861 ggatacgtcc gcaaggacgc ccagccggaa gcgctgctcg acgcgatcag ggcggtcgcg
    10921 cgaggcaaga cctacatcga cccgtcggtt gcccggcgcg tcatcgttgg cgcggggccg
    10981 cgcgacgatc tcaccggccg cgaggtggag gtgctccgcg cgatcgggct gggccagacg
    11041 aacaagcaga tcgccgagca gcttggcgtc accgaggaga cggtcaagac gcacgttgcc
    11101 agcctgctcg cgaagctcga ggtggacaat cgcacgcagg cggtggtcga ggccattcgc
    11161 cgcgggatcg tcggcgtgga ggaactcgag taggcaagcg gcggcgggat ccggcgtcag
    11221 gctccagggg taagatagcg cggaccatgc cccatcacgc gtcgattcac gcgctcgttg
    11281 ccgatctggc cagtctcggc gtcgccaccg gcgacctcgt catggtgcac gcgtcgctcc
    11341 gcaggctcgg tcccgtcgag aggggagccg agggagtgat cgaggcgctg gacgaggcgg
    11401 tcggtcctgg cggaaccgtg atgatggtgc tcggggcccg cgacgactgg gcctgggtga
    11461 acgaccgccc ggagccggaa cgggccgcgc tgctcgaggg ctctccgccc ttcgacgcgc
    11521 tggccacgcc agcctcgccc gaggtcggcg tgctggccga ggtgtttcgg cgcacgccgg
    11581 ggacgcgcgt gtccgaccac ccggaaggcc ggttcggtgc gcgcggcctg ctggcccgcc
    11641 acctgttgct cgacgtgccc tgggacgact actacggtcc cggctcgccg ctcgagcgcc
    11701 tcgtggatgg gggcgggaag gtgctccgcc tcggggccga ccccaacacc gtgaccctaa
    11761 tccactatgc cgagtacctc gcggaggtgc ccggcaagcg ccgggtcagg cgccaccggc
    11821 tggtggccac cccccgtggt ccggagctgc gcattgtcga gtgcctcgac gacaacaacg
    11881 ggatcgtgga ctacccggga ggcgattact tcgccgacat cctgcaggcc tatctcgagc
    11941 agggcagggc gcgccaggga ctggtcggcc aggccggcag cgaactgctc gacgccagag
    12001 atttggcaga gttcgcggcg cgctggatga cgaagcggtt cgggagcgga ggggcaacac
    12061 cgcctgccgg ctgaccgggc cgggcccggc gcggcccacg ccttcagggc tacaatggat
    12121 gccgccttgg cccgccttcg ctgcttcgca gcttcggcga ggggggcagg cgtgccggaa
    12181 atcagccgtc gttcctggag gagagcatgt cgcatttcat cggcgagaag gagttcttcc
    12241 cgggcatccc cgccatcaag tacgaggggc gcgactcgac caaccccctc agcttcaagt
    12301 tctacgaccg cgacaagaag gtgctgggca agacgatggg ggagcacctg cggttcgcgg
    12361 tggcgtactg gcactcgttc tgcgccgagg ggtcggacca gttcggcgac gggacgcgag
    12421 tcttcccgtg gggatcggcc ccgacgccga tggaccgcgc gcggcagaag ctcgacgccg
    12481 ccttcgagtt catcaccaag ctgggagcgg ccttctactg cttccacgac cgcgacatcg
    12541 cgcccgccgg cgcgacggtt gccgagtcgg agaagaacct gcagacgctc gtcgcgctcg
    12601 ccaaggagcg gcagcaggca acgggcgtcc agctgctgtg ggggacggcg aacctgttct
    12661 cgcacccgcg ctacatgaac ggcgccgcga ccaatcccga cttccgggtg gtcacgcacg
    12721 ccgccgcgca ggtgaaggcg gccatcgacg cgacaatcgc gctgggcggg cagggctacg
    12781 tcttctgggg cggccgcgag ggctacacga cgctgctgaa caccgacatg aagcgcgagc
    12841 tcgagaacct cgggcgcttc ctgtcgatgg cgcgcgacta cgggcgccgc cagggcttca
    12901 ccggcacctt ctacatcgag ccgaagccga tggagcccac caagcaccag tacgacttcg
    12961 acgcggcgac ggtgatcggc ttcctgcgcg cgcacgggct cgagaaggac ttcaagctca
    13021 acatcgaggc gaaccacgcg acgctggcgg gccactcgtt ctcgcacgag ctgcaggtgg
    13081 cggccgacgc ggggctgctc ggcagcatcg acgccaaccg gggcgatccg cagaacgggt
    13141 gggacaccga ccagttcccg acggacctct accaggccac cgaggcgatg atgatcgtcc
    13201 tgcgggcggg cggcttcacg acgggcggcc tgaacttcga cgcgcacatc cggaggaact
    13261 cgaccgatcc cgaggacctg ttcctcgcgc acatcggcgg gatggacacc ttcgcgcggg
    13321 cgctcgagat cgcggcacgg ctgctcaacg agtcgccgct caaggacctg acgaagaagc
    13381 gctacgcctc gttcgacgag gggaacggct tgcgcttcac caagggcgag ctgacgctcg
    13441 aggacctggc ccggctcgcc gggcagggcg agccgccgct ggtcagcggc aagcaggagc
    13501 tgatcgagaa cgtgatcaat cagtacctgt gagctgacag cgtgggggcg accctcgtgg
    13561 tcgcccctac agcgtctctg gcaggtggag ccgcaggcca ttcgctccga aaaccgggcg
    13621 acggcactcg cggccctgcg cggggagagg gcgcgagccg ccgtgggccc ttaccgcgca
    13681 cgcacgggca gcgttccgga gtcgacggtt tcgcctgtgc gggaaatcgc ctggaagtgc
    13741 atcgtgtcgc cggcgatctc gaccagcatg aacgaccggt cgcgatcgaa gccggcggcg
    13801 gtcagcgccg tgtcctggat gtcgccccgc cgcagcttgc ccgccccgcc cgacgtgaag
    13861 taggtgattc ccttttgcgg cttgatccgc tcgtagaagt gctcgtggcc ggcaaagacg
    13921 gcgctcacgc cgtgctcgac gaacagcggc tcgaggatcg cgcgcagggt cgtgtccgac
    13981 ccgtgcttcc ccccggatga gtacagcgga tggtggaagt aggcgatctt ccaccgtgcc
    14041 tgcgaggcgg ccagctcgcg gcgcagccag tcgacctgct tcgcgtcgag gtcggtcgtg
    14101 tcgaggacga agaactggac cggccccctg gtgaacgagt agtaccgctc gccgtccatg
    14161 ttgaacggcc cgtacagccg ctgctccggc tcgtcgtggt tgccgagcgc ggcgtagaac
    14221 cggacgcccg cgtcgagtag ctccttgtag gggatttcga acttccgcct gtagtcctcg
    14281 ggccgctccg atccgtacag gttgtcgccc agcatgatca cgaagtcgaa cgggaactgg
    14341 gcgtgcgcct cgaccagccg gccgatgagc cgggcg
//
LOCUS       DAOPQX010000055        14350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_4944_length_14350_cov_3.764883, whole genome shotgun sequence.
ACCESSION   DAOPQX010000055 DAOPQX010000000
VERSION     DAOPQX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14350
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4944_length_14350_cov_3.764883"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1939)
                     /locus_tag="PKK95_04795"
     CDS             complement(203..1939)
                     /locus_tag="PKK95_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17562.1"
                     /translation="MSSSDASVSPHVPLRSGRSRRLRTLAGIAMAVSLLAALFGGAGT
                     RAAAPRFYPDDPIQVDNDREFDASRAAPVEGSNVWDFVENTFFEKGERNSIRALNVNT
                     MDEVPDSSWFTNRIGRVDLSPAEIGRGPNTLDVVNIDGWPIVEGKSQGVTPGYRVVDP
                     SGRRYQIKFDPPSNPEMASGAEMIGAALYHAIGYNVVEGYLVEVDPDRIVIGPEATTV
                     DLSGRKKRMTMEDVKLILRRAARGPNGRYRALASRYAEGKYLGYFKYYGTRADDPNDI
                     FPHEHRRELRGNRVFAAWLNHDDSRGLNSLDMLQGSEGRRFVRHYMFDFGSIMGSGST
                     KPQAPRAGNEYMLEWKPSLLTLVTLGFYVRPWITIDYPEVAKSIGRFESARFDPVRWK
                     PEYPNPAFENMRADDAFWAARIVAKFSDEGVRAAVARAQYREPGAAEYAAKTLIERRD
                     KVLRAWLPGVNPIVSPALDDEGVFTFRNAAVDAGLAGKPAGYLVQWHQFDNADGDGPG
                     DAIGPEVRVTEPRAAAPLESLKGTEFAAARVRTEHAEFPHWADPVTVYFRREADGWRA
                     VGLERQEPREVQ"
     gene            complement(1929..3161)
                     /locus_tag="PKK95_04800"
     CDS             complement(1929..3161)
                     /locus_tag="PKK95_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOC17563.1"
                     /translation="MKPSTLRTLSIVALACALAGPTAAQTPETREEELRARREAKAKE
                     LEKERQGRVERFLVSLENDRIFERLLNPPEGVYPLIGHVTPGSGLSLGVGYRQPRLFD
                     ERAVFQASAAGSPRKYWLIDTRLLLPRLANEAAQVEFYAHRSWWPEEAFFGLGPDSLR
                     REHANFSLGNTVVGGSGAVRTGRLLTLGGRAEYLRPRSGRGRAKSIPNAGTLFPGEEI
                     PGFGASTNFGRFEAFADFNYREPLMNPRRGGRYYVSYALFDDLHLDRYGFRRLEVDLQ
                     QYVPFLRDRRVFAFRALLSASDAVAGQQVPFYLMRSLGGPDDLRGFRRHRFRDANLLL
                     FQAEYRWEVFTAVDAALFVDAGQVAPRVSDFDLEDLETDYGFGLRFGSVNGVFLRVEG
                     AFGSRDGKHFILRFGHVF"
     gene            complement(3168..4502)
                     /locus_tag="PKK95_04805"
     CDS             complement(3168..4502)
                     /locus_tag="PKK95_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17564.1"
                     /translation="MGQPENVARSVPWWSRLLSPIADIRPGEAATVLLLGVNVFCLLA
                     LYYVLKTVREALILTESGAEVKSYAAAGQALLLLVAVPAYAALASRVNRVWLVGGVTL
                     FFASHLLVFYALGMASVPVGVPFFLWLGVFNLVVIAQFWAFANDLYSREAGKRLFPLI
                     GLGSALGAWLGAQFASSLFAAIGPFHLLLVAAGGLVFCVLVTIWVHEREKGREEGAAG
                     EGERPLGKEGAFRLIFRDRYLLLIALLLVLVNVVNTVGEFLLGSLVVQEASRVAAAQG
                     IERGVFIGRFYGSFFAWVNLLGFLFQLLLVPRLFKHLGVNGTLFVLPIVALLSYGTLV
                     FVPILGVVRLMKTLENASDYSIQMTTRHALFLPTSREAKYKAKQAIDSFFWRAGDLLQ
                     AGVVFFGARLGFGMRDYALLNIVFVGVWLVIVVAIARDYRERIKARPARAVA"
     gene            complement(4516..6156)
                     /locus_tag="PKK95_04810"
     CDS             complement(4516..6156)
                     /locus_tag="PKK95_04810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC17565.1"
                     /translation="MKESEAQVSRRMEPAFTPWSGTDGTGWALPEDVLQETSRRLSTL
                     AVVAAVLWGIALVINNLFARGFAGINTHPYPWPGNLVSGIAVLLAVAMGVYARLAGAG
                     RAALDLGLAFLVVNAFALGLLDEWVEREIRVRSVSWIAVVILIYAMIAPSTPRKTLLA
                     SLVAASMDPLGALIAHLRGLPVPRASEVLLQYWPNYAAAFLAVVPSQVLHRLGRQIAK
                     VRDAGSYQLVQELGRGGMGEVWRAEHRLLARPAAVKIVRPEMLDVPNEAEVRLLMQRF
                     MREAQATSVLSSPHTIELYDFGITNDGAFYYVMELLVGRDLETLVRDFGPLPASRTVY
                     LLRQVCHSLAEAHSRDLVHRDIKPANVYVCRMGLDYDFVKVLDFGLVTFGDQAGFRRT
                     ALNADRVTSGTPAYMAPEVILGETEIDRRADVYALGCVAYWMLTGQLVFEADTRMRMF
                     MDHLQATPVPPSQRTELPIPRELDELVMRCLEKDPEKRPRDAGELFEMACSCKAHDAW
                     DNDAAREWWELHLSEFTQPLTLAVPAAPAVPPAAAVHS"
     gene            complement(6187..8487)
                     /locus_tag="PKK95_04815"
     CDS             complement(6187..8487)
                     /locus_tag="PKK95_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17566.1"
                     /translation="MKPSPSFPASAVLLAMLGAAAVIAQFVAGKATRDALFLARFEVT
                     QLPLMVMAASAFSIACAFAASRAMGRLSPARLLPIAFVGSAVLILLQWGLAATSPRVT
                     AVLFYLHMLGLAPVLVSGFWTLLSERFDPRTAKRRLGHVGAAGTFGGVAGTVIADRAA
                     VWGSAGSVLPVLVVLNLACAWIVWRLALLKAGGTPRPHARAGRGAAAPKANPRLAKPS
                     GLQVVLGSAHLRNLAVLVLLTTLGAVLVDYVFKARAVAGIGRGNELLRFFALYYGATS
                     VLTFLVQVTLSRPVLEKAGLAPAVGAPAMAVVAGSLGSLILPGLPPAAAARGTEYVSR
                     MSLFRSAYELLYTPVSAREKRAAKPIIDVGFDRAGDLVGAGLAGILVGTAASHSIILA
                     VALMAAVTALVVARRVTRGYIQTLERSLLDQAVLLDLSEIEDLTTRETVHRTLAGISA
                     LAVAPAAARPAPAPAAPAAVTAVPADPLLQKDPVLQKIAALRSRDRERVLKVLHEERG
                     PERALVPHVIPLLAWDAVASDAIVALKKAAEEVVGQLVDSMLDPRQDFAIRRRIARVL
                     SICVSQRAADGLLAALDDRFEVRFQAGRSLAAIVERNGRVRIDRDRIYQVVLREVSVG
                     RPVWESHRLLDSVEEQDGTFFADRFIRERAGQSMAHVFTLLSLVLHREPLQIAFRGLH
                     VQDPLLRGTALEYLESVLPPEIRTRLWPFLEDSRPSARPRRSRDQIVADLVRSNDSIL
                     LNLAELERQGRGVMKEPPEDVAVGQG"
     gene            complement(8783..10231)
                     /locus_tag="PKK95_04820"
     CDS             complement(8783..10231)
                     /locus_tag="PKK95_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17567.1"
                     /translation="MNTTSQAERHGITRREFVGGALTAAGVVAGAPALLRGRNLNDKL
                     DIAFIGCGGRGRANLRELVIVPGQPAAQGGRRDAAPNVPHPDENVAVLCDIDQVALDA
                     GAQQFPKAKTFTDLRRVFDEPNAFDAVVVSTAEHTHVFATYLALTHGKHVYCEKPLTR
                     NIWEARLIRELAAKYPKLSTQMGNQGHASPSRRAIKEILMTGVIGPVREVHVWAERAW
                     GLQDAASAEKFDKPHGFYNGIQIVDRFKEEMPVPPTLHFDLWLGPAPVRPYHATYFPG
                     PRWYRWWDFGNGTMSDLGCHDNDVPFTVLDIRQPLTVEARSPNVPVAHKELAPATMMV
                     TFEFPAAGGNPPVKLVWYQGDVKPPGWVPEWGGRSQLFVGEKGMLLGNGKLLPEEKFK
                     DFQPPPETLPRSPGHWVEWVNYAKGLGPVPGSNFQYSGWTTEANHLGNVAYRSGKKIE
                     WDYQNLRARNAPEAAPFIKRPEYRKGWEGILK"
     gene            10412..11071
                     /locus_tag="PKK95_04825"
     CDS             10412..11071
                     /locus_tag="PKK95_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOC17568.1"
                     /translation="MNDRVRTLAGVLWQYHQLGHPLEKADAILVLCSHDKAVAERGAR
                     LFLERWAPLLIFSGGLGAITRHLWDRPEADQFAEVARAMGVPADRILVENRSTNTGEN
                     VAFTRRLLEERGLDPGSFILVQKPYMERRSYATFQKVWPGKRLIVTSPRVSLDEYLER
                     YSNETLSADDVVSIMVGDLQRIRLYPEKGFQVPQEIPPEVWEAYRELVSLGYDRHLVR
                     G"
     gene            11155..11916
                     /locus_tag="PKK95_04830"
     CDS             11155..11916
                     /locus_tag="PKK95_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOC17569.1"
                     /translation="MPTATRTPLFKPRTLSFLRALKRNNDRAWFAEHKEEYLRDVHQP
                     MLDFVAWVASELPAFAPELEASPRTSLFRIYRDTRFSADKTPFKTQVGAIFPQRGLHR
                     NVSACLYVEIGPAGAMIFGGIYKPERSELLAIREHIARTHRRLRALLEAPAFKRTVGA
                     MEGDALQRVPPGYAPDHPAAEYLKFRQFLLGKEYPAAFATSPEFPAEILRLFRRMAHV
                     VGYLNEPLLAAERARDPLAERSPRREPKRVSRLIG"
     gene            complement(11986..12195)
                     /locus_tag="PKK95_04835"
     CDS             complement(11986..12195)
                     /locus_tag="PKK95_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17570.1"
                     /translation="MRVAIFGAFGTIGRAAAAELLRRGHGVRMSYLQTTPVLLDDSAL
                     GSVLPLRKTPYDEGIRLTLDHLRNR"
     gene            12413..>14350
                     /locus_tag="PKK95_04840"
     CDS             12413..>14350
                     /locus_tag="PKK95_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17571.1"
                     /translation="MQEIRQAMRALSSRPSLVVVSVLTLALGIGGTTAVFSVVDAVLL
                     RPLPFSGSDRLVSLTESSPNLKTLSVSWQNYVDWRHQSRSFESVAAYRPINVTITGEG
                     EPERVPAKMVSAAMLPMLGVKPSLGRSFTKEEDGAGGEPVALISEGLWTRRFARDPRA
                     VGQVMTIDNTARTIVGVLPASFVLVQPADVLIPIGPWASTLPDDRGWHPGIFPLARLK
                     NGVELGKARLEMAQIAKNLERQYPETNLKVGADVTPLQQQMVQNVQPALAVLLGAVVL
                     VLLIACANVANLLLVRTIERRREIAVRAALGARRWRLIRHVLAESLVLALAGGLLGVL
                     VASWAVSALVALAGPGLPRADSIAVDQRVLLFALALSTLTALAFGAAPAVHASRADIR
                     EALNEEGRGTTSAGSRRLARAFVVAEVALATTLLVGAGLLLRSFVRLQQVPPGFSAAR
                     LLVADLPLSPIDYAEDAARERFVDRLLERVTALPGVEAAGTTTTLPMAGSGALLHFNI
                     YGRPPKGPEDYILAGYRAVSPTYLAALGVPTLRGRPFTEWDRSGSKRVVVINETMAKT
                     FFKGSDPLGQRISIGTEPDEESPWMEVVGVVGDVRQSFEADPKAEMYVPYLQGAPHPV
                     LAGLFRSVSVVVRAKGDPLQVA"
BASE COUNT         2234 a         5025 c         5005 g         2086 t
ORIGIN      
        1 tgcgggtgcc gccgcgcacg cggcgtcgga cgaagggcag agggtgggaa ccagagtggg
       61 gaacaacggc acggccgcgt gtcccggttg agcaaagccg ggcaccgcgc aaaacgacca
      121 gacgaccaga acgattcctg cccgaaggct gaccgttgct cttctcacgc ccgtcctccc
      181 acggccccgg ccgcgctcac tgtcactgga cctctcgcgg ctcctgccgc tccagcccaa
      241 ccgcccgcca tccgtccgcc tcgcgccgga aataaacggt caccgggtct gcccagtgag
      301 ggaactccgc gtgctccgtg cgaaccctcg ccgccgcaaa ctccgtaccc ttcaggctct
      361 ccaacggcgc cgccgcccgc ggctcggtga cccggacctc ggggccgatg gcatcgcccg
      421 gtccgtcgcc gtccgcgttg tcgaactggt gccactgcac caggtacccg gccggcttcc
      481 cggccaggcc cgcatcgacg gcggcgttac gaaacgtgaa aacgccttcg tcatcgagcg
      541 ccggcgacac gatcgggttg acgcccggca gccaggcgcg gagcaccttg tcgcgccgct
      601 cgatcagcgt tttcgccgca tactccgccg ccccaggctc cctgtattgc gcgcgcgcca
      661 ccgctgcacg aacgccctcg tccgagaact tggcgacgat ccgcgcagcc cagaacgcat
      721 cgtcggcacg catgttctcg aaggccgggt tggggtactc gggcttccag cgcaccgggt
      781 cgaaccgggc cgactcgaag cggccgatcg atttcgccac ttccgggtag tcgatcgtga
      841 tccagggccg gacgtagaaa ccgagcgtga ccagcgtgag cagcgacggc ttccactcga
      901 gcatgtactc gttgccggcc cgaggggctt gcggcttggt gctcccgctc cccatgatgg
      961 agccgaagtc gaacatgtag tgtcgcacga accggcgccc ttccgagccc tgcagcatgt
     1021 ccaggctgtt gagccccctc gagtcgtcgt ggttgagcca ggcggcgaac acccggttgc
     1081 ccctcagctc gcgccggtgc tcgtgcggga agatgtcgtt ggggtcgtcg gcgcgcgtcc
     1141 cgtagtactt gaagtagcca aggtacttcc cttccgcgta acggctcgcg agcgcgcgat
     1201 acctgccgtt cggccctcgg gccgccctcc gcaggatcag cttcacgtcc tccatcgtca
     1261 tccgcttctt ccggccggag aggtcaaccg tggtggcctc ggggccgatg acgatgcggt
     1321 ccggatccac ctcgacaagg tagccttcga cgacgttgta gccgatggcg tgatagaggg
     1381 cagcgccaat catctcggcg ccgctcgcca tctcggggtt gctgggcggg tcgaacttga
     1441 tctggtagcg ccggcccgac gggtcgacca cgcggtagcc cggggtcacg ccctggctct
     1501 tgccctcgac gatcggccag ccgtcgatgt tcaccacgtc gagcgtgttc ggcccgcggc
     1561 cgatctccgc cggggagaga tcgacacggc cgatccggtt ggtgaaccag ctcgagtcgg
     1621 gcacctcgtc catggtgttc acgttgagcg cccggatgga attccgctcg cccttctcga
     1681 agaaggtgtt ctcgacgaag tcccagacgt tgctgccctc gacgggggcg gcgcggctcg
     1741 cgtcgaactc gcggtcgttg tccacctgga tggggtcgtc cgggtagaag cgtggggcgg
     1801 cggcgcgggt gcccgctccc ccgaacagcg ccgccagcag ggagacggcc atcgcgatgc
     1861 ccgcgagcgt gcgcagcctg cgcgagcggc cggagcgaag cgggacgtgc ggggagaccg
     1921 aagcgtcgct agaagacatg gccgaacctg aggatgaagt gcttgccgtc gcggctgccg
     1981 aatgccccct cgacgcgaag gaacacgcca ttgaccgagc cgaacctgag gccgaagccg
     2041 tagtcggttt cgaggtcctc gagatcgaag tcggagaccc gcggcgccac ctggcccgcg
     2101 tccacgaaga gggcggcgtc gaccgccgtg aacacctccc agcggtactc ggcctggaag
     2161 agcaacaggt tcgcgtcccg gaaccggtgc cggcggaagc cgcggaggtc gtcggggccg
     2221 ccgaggctcc gcatcaggta gaagggcacc tgctgcccgg caacggcgtc cgaggccgac
     2281 agcagcgccc ggaacgcgaa gaccctgcgg tcccggagga agggcacgta ctgctggaga
     2341 tcgacctcca ggcggcggaa gccgtagcgg tcgaggtgga ggtcgtcgaa cagcgcgtag
     2401 ctcacgtagt agcggccgcc gcgccgcggg ttcatcagcg gctcgcggta gttgaaatcg
     2461 gcgaacgcct cgaagcggcc gaagttggtg gacgcgccga agcctggaat ctcctcaccc
     2521 gggaagagcg tcccggcgtt gggtatgctc ttcgcgcgtc cccgccccga ccgcggccgc
     2581 aggtactcgg cgcggccgcc cagtgtgagc agcctgcccg ttctcaccgc gccgctgccg
     2641 ccgactaccg tgttgccgag gctgaagttg gcgtgctcgc ggcggagcga gtcggggccg
     2701 aggccgaaga acgcctcctc gggccaccac gagcggtggg cgtagaactc cacctgcgcg
     2761 gcctcgttgg ccaggcgcgg cagcagcaac cgggtgtcga tcagccagta cttcctcggc
     2821 gatccggccg ccgaggcctg gaacaccgcc cgctcgtcga acaggcgggg ctgccggtat
     2881 ccaacgccca gcgagaggcc gctgccggga gtcacatgtc cgatcagcgg gtagactccc
     2941 tccgggggat tgagcagccg ctcgaagatc cggtcgttct ccaggctgac gaggaagcgt
     3001 tcgacccggc cctgccgctc cttctccagc tccttcgcct tggcctcccg ccgggcgcgc
     3061 aactcctcct cgcgcgtctc gggagtctgg gcggcggtgg ggccggcgag cgcgcacgcc
     3121 agggccacga tcgagagggt gcggagcgtc gagggcttca tggggcgtta cgcaactgca
     3181 cgggccggcc gcgccttgat gcgctcgcgg tagtcgcgcg cgatcgcgac gacgatcacc
     3241 agccacacgc caacgaacac gatgttgagc agcgcgtagt cgcgcatgcc gaagccgagc
     3301 cgggcaccga aaaagacgac gcccgcctgc agcaggtcgc cggctcgcca gaagaacgag
     3361 tcgatcgcct gcttcgcctt gtatttcgcc tcgcgcgagg tgggcaggaa cagcgcgtgc
     3421 ctcgtggtca tctggatcga gtagtcggag gcgttctcga gcgtcttcat caggcggacg
     3481 acgccgagga tgggcacgaa gaccagcgtg ccgtagctga ggagcgccac gatggggagc
     3541 acgaagagcg tgccgtttac gccgaggtgc ttgaagagcc gcgggacgag cagcagctgg
     3601 aagaggaacc cgagcaggtt gacccacgcg aagaagctgc cgtagaagcg cccgatgaac
     3661 acgccccgct cgatcccttg cgcggcggcc acccgcgagg cctcttgcac gacgagcgat
     3721 ccgaggagga actcgcccac ggtgttcacg acgttgacca gcacgaggag cagcgcgatc
     3781 agcagcaggt agcggtcgcg gaagatcagc cggaacgccc cctctttgcc cagcgggcgc
     3841 tcgccctccc ccgccgcgcc ctcctcgcgc cccttctcgc gctcgtgcac ccagatggtg
     3901 accagcacgc agaagaccag gccgccggcg gcgaccagca ggagatggaa cgggccgatt
     3961 gccgcgaaga gcgacgaggc gaactgcgcc ccgagccacg ccccgagggc gctgcccagc
     4021 ccgatcagcg ggaagaggcg cttgccggcc tcccgcgagt acaggtcgtt ggcgaaggcc
     4081 cagaactggg cgatcaccac caggttgaag acgccgagcc agaggaagaa cggcacgccg
     4141 accggcacgc tcgccatgcc cagcgcgtag aacaccagga ggtgcgaggc gaagaagagg
     4201 gtcacgccgc ccaccagcca cacgcggttc acgcgcgagg ccagggcggc gtacgcgggc
     4261 accgcgacca gcagcaacag cgcttggccg gccgccgcgt agctcttcac ctcggcgccg
     4321 ctctcggtga ggatcagcgc ctcgcggacg gtcttgagga cgtagtacag cgcgagcagg
     4381 cagaagacgt tgacgcccaa caggagaacc gttgccgctt cgccaggccg gatgtcggcg
     4441 atcggggaga gaagccgcga ccaccagggg acggaccggg caacgttctc cggctgaccc
     4501 atctgcgacc tccaatcacg aatggacggc ggcggccggc ggcacggccg gcgcagccgg
     4561 gaccgccagc gtcagcggct gggtgaactc cgacaggtgg agctcccacc actcgcgcgc
     4621 ggcgtcgttg tcccaggcgt cgtgggcctt gcacgagcag gccatctcga acagctcgcc
     4681 ggcgtcccgg gggcgcttct cggggtcctt ctccaggcag cgcatgacca gctcgtccag
     4741 ctcgcgcggg atcggcagct cggttcgctg cgaggggggg acgggggtgg cctgcaggtg
     4801 gtccatgaac atccgcatgc gcgtgtcggc ctcgaacacg agctggcccg tcagcatcca
     4861 gtaggcgacg caccccagcg cgtagacgtc ggcgcgccga tcgatctcgg tctcgccgag
     4921 gatcacctcg ggggccatgt aggccggcgt gcccgaggtt acgcgatcgg cgttcagcgc
     4981 ggtgcgccgg aagccggcct ggtcgccgaa ggtcaccagg ccgaagtcga gcaccttgac
     5041 gaagtcgtag tcgagcccca tgcggcagac gtagacgttg gccggcttga tgtcgcggtg
     5101 gaccaggtcg cggctgtgcg cctcggccag cgagtggcac acctggcgca ggaggtacac
     5161 ggtgcggctg gcgggcagcg ggccgaagtc gcggacgagg gtctcgaggt cgcgcccgac
     5221 cagcagctcc atcacgtagt agaaggcgcc gtcgttggtg atgccgaagt cgtagagctc
     5281 gatggtgtgg ggcgaggaga gcaccgaggt cgcctgcgcc tcgcgcatga agcgctgcat
     5341 gagcagcctg acctcggcct cgtttggcac gtccagcatc tccggccgga cgatcttgac
     5401 cgccgccggc cgcgcgagca gccggtgctc ggcccgccag acctcgccca tcccgcctcg
     5461 ccccagctcc tgcaccagct ggtagctgcc cgcatcgcgc accttggcga tctgccgccc
     5521 cagccggtgg agcacctgcg aggggacgac cgcgaggaag gccgcggcat agttgggcca
     5581 gtattggaga aggacctcgc tcgcccgggg gacgggcagt ccccgcaaat gcgcgatcag
     5641 tgcgccgagc gggtccatcg aagccgccac cagcgaggcg agcagggtct tgcgcggcgt
     5701 gctgggggcg atcatcgcgt agatcaggat caccaccgcg atccacgaca ccgagcggac
     5761 gcggatctct cgctccaccc actcgtcgag caggccgagc gcgaacgcgt tgaccaccag
     5821 gaatgccagg ccgaggtcga gcgccgcccg gccggccccg gcgagacggg cgtagacccc
     5881 cattgccacc gcgaggagga cggcgatgcc gctgaccagg ttcccgggcc acgggtaggg
     5941 atgggtgttg atgccggcga agccgcgcgc aaacaggttg ttgatgacga gcgcgatgcc
     6001 ccacagcacc gccgccacca cggcgagggt ggacagacgg cgcgaggttt cctgcaggac
     6061 gtcttcgggc agcgcccagc ctgtgccgtc ggtgccgctc cagggcgtga acgccggctc
     6121 catgcgccgc gacacctgcg cctcggattc cttcatcgct ccctcatcgg ctcctcaccg
     6181 gccgcgtcac ccctggccca cggcgacatc ctcgggcggc tccttcatca cgccccggcc
     6241 ctggcgctcg agctcggcaa ggttcaacag gatcgagtcg ttcgaccgca ccaggtcggc
     6301 gacgatctgg tcgcgcgacc ggcggggccg ggcggagggc cgcgaatcct ccaggaaggg
     6361 ccagaggcgc gtacgaatct cggggggcag cacgctctcg aggtactcga gcgccgtgcc
     6421 gcgaagcagg gggtcctgca cgtgcaggcc gcgaaacgcg atctgcagcg gctcgcggtg
     6481 cagcaccagc gagagcagcg tgaagacgtg cgccatgctc tgcccggccc gctcgcggat
     6541 gaagcggtcc gcgaagaagg tgccgtcctg ctcctccacg ctgtcgagca gccggtggct
     6601 ctcccacaca ggacggccca cgctcacctc gcgcagcacc acctggtaga tgcgatcgcg
     6661 gtcgatccga acgcgcccgt tgcgctcgac gatcgccgcc agcgagcggc ccgcctggaa
     6721 gcggacctcg aaccggtcgt cgagggccgc cagcaggccg tcggccgcgc gctgcgacac
     6781 gcagatcgag agcacgcggg cgatgcggcg ccggatggcg aagtcctgcc tcgggtcgag
     6841 catcgagtcc accagctgcc ccaccacctc ctcggccgcc ttcttcagcg ccacgatcgc
     6901 gtcggacgcc accgcgtccc acgcgagcag cgggatgacg tgcgggacga gggcgcgttc
     6961 cgggccacgc tcctcgtgca gcaccttcag cacacgttcg cgatcgcgcg atcgcagggc
     7021 ggcgatcttc tgaaggacgg ggtccttctg aaggaggggg tcggctggca cggccgtcac
     7081 ggcggccggc gccgcaggcg caggcgcggg ccgcgcggcc gccggcgcaa ccgccagcgc
     7141 cgagatcccg gccagcgtcc ggtgcaccgt ctcgcgggtg gtcaggtctt cgatctcgct
     7201 caggtcgagc aggacggcct ggtcgagcag gctgcgctcg agcgtctgga tgtacccgcg
     7261 cgtcacccgg cgagccacca ccagtgccgt caccgcagcc atcagcgcga ccgccaggat
     7321 gatggagtgg ctcgcggcgg tgccgacgag gattccggcg agtcccgcgc cgaccaggtc
     7381 gccggcccga tcgaagccga cgtcgatgat tggcttggcc gcccgtttct cacgcgcgga
     7441 gacgggcgtg tagagcagct cgtaggccga gcggaagagc gacatgcggg agacatactc
     7501 ggtcccgcgt gcggcggcag ccggcggaag gcccggcaga atgagcgacc cgaggctgcc
     7561 tgcgaccacc gccatagcgg gtgcgccgac cgcgggcgcc agcccggcct tctccagcac
     7621 cggccggctg agcgtgacct gcaccaggaa ggtcaggacg ctggtcgcgc cgtagtagag
     7681 cgcgaagaag cgcagcagct cgttgccccg cccgatcccg gccaccgccc gcgccttgaa
     7741 cacgtagtcc accagcacgg cgccaagcgt ggtcagcagc accagcacgg cgaggttccg
     7801 caggtgcgcg cttcccaaaa ccacctggag gcccgacggc ttcgcgagcc gggggtttgc
     7861 cttcggtgcc gcggcgcccc tgccggctcg tgcgtgcgga cggggggtcc cgcctgcctt
     7921 gagcaaggcc agccgccaca cgatccaggc gcacgccagg ttgagaacca ccaggaccgg
     7981 cagcaccgag ccggcactgc cccagacggc cgcccggtcg gcaatcacgg tgcccgccac
     8041 accgccgaag gtgccggccg ccccgacgtg accgagccgg cgcttggccg tgcgcggatc
     8101 gaaccgctcg ctcagcagcg tccagaaccc cgaaaccagc acgggcgcca gcccgagcat
     8161 gtgcaggtag aacaggaccg ccgtgacgcg cgggctggtg gcggcgaggc cccattggag
     8221 caggatgagg acggcgctgc ccacgaaggc gatcggaagc aggcgcgccg gcgacaggcg
     8281 ccccatcgcg cgcgaggcgg cgaacgcgca cgcgatggaa aaggccgagg ccgccatcac
     8341 catcagcggc agctgggtga cctcgaagcg ggcgaggaag agggcgtcgc gcgtggcctt
     8401 ccccgccacg aactgcgcga tcaccgccgc ggcgccaagc atcgcgagca gaaccgccga
     8461 ggcgggaaag gatggcgagg gcttcaatgg gggcgctcga agctgaagcg cacggccgcg
     8521 cggacgaaaa gccgggcgcg aaacggacgc gtcggacttc gacctgcaaa ctgcgcgccg
     8581 aggccagctc cgccgttccg aaggcctaac ccctttgacg ctcgacagtt agcaccgtat
     8641 ccacccgttg ctgccgccgc cggcgcattg agactctgat cgtcgcgtag cgaagggcgt
     8701 gtagggtctt gctacgtaag ccgatccttg aggcaggagc aggggcacgg ctgccgtgcc
     8761 cctgccagcg acgagcggtg gtctacttca ggatcccttc ccaccccttc cggtactcgg
     8821 gccgcttgat gaatggcgcg gcctcgggcg cgttgcgggc gcgcaggttc tggtagtccc
     8881 actcgatctt cttcccgctg cggtacgcga cgttgcccag gtggttcgcc tcggtggtcc
     8941 agccggagta ctggaagttg gagccgggaa ccgggccgag ccccttcgcg tagttgaccc
     9001 actccaccca gtggcccggc gagcgcggca gcgtctcggg cggcggctgg aagtccttga
     9061 acttctcctc gggcaggagc ttcccgttgc cgagcagcat ccccttctcg ccgacgaaca
     9121 gctgcgagcg gccgccccac tcgggcaccc agccgggagg cttgacgtcg ccctggtacc
     9181 acaccagctt caccggcggg ttgcctccgg ccgctgggaa ctcgaatgtc accatcatcg
     9241 tcgcgggcgc cagctccttg tgcgccaccg ggacgttggg cgacctggcc tcgacggtga
     9301 gcggctgccg gatgtcgagg acggtgaacg ggacgtcgtt gtcgtggcag ccaaggtcgc
     9361 tcatcgtccc gttgccgaag tcccaccagc ggtaccagcg cggccccggg aagtaggtgg
     9421 cgtggtacgg gcgcacgggc gccgggccga gccagagatc gaagtgcagg gttggcggga
     9481 ccggcatctc ctccttgaag cgatcgacga tctggatgcc gttgtagaag ccgtgcggct
     9541 tgtcgaactt ctcggccgag gcggcgtcct ggagccccca cgcccgctcg gcccacacgt
     9601 ggacctcgcg cacgggaccg atgacgcccg tcatgaggat ttccttgatc gcgcggcgcg
     9661 agggagaggc gtgcccctgg ttgcccatct gcgtcgagag cttcgggtac ttcgccgcca
     9721 gctcgcgaat gagccgcgcc tcccagatgt tgcgagtgag cggcttctcg cagtagacgt
     9781 gcttgccgtg ggtgagggcg aggtaggttg cgaagacgtg cgtgtgctcg gccgtggaga
     9841 cgacgaccgc gtcgaaggcg ttcggctcgt cgaagacgcg gcgcaggtcg gtgaacgtct
     9901 tcgcctttgg gaactgctgc gcgccggcgt cgagcgccac ctggtcgatg tcgcacagga
     9961 ccgccacgtt ctcgtcgggg tgcggcacgt tcggcgcggc gtcgcggcgc cccccctgtg
    10021 cggctggctg cccgggcacg atgaccagct cgcgcaggtt tgcgcggccg cgtcccccgc
    10081 agccgatgaa ggcgatatcc agcttgtcgt tcaggttccg gccccgcagg agggccggcg
    10141 cgccggcaac caccccggcg gccgtcaggg ccccgcccac gaactcgcgg cgcgtgatgc
    10201 cgtgccgctc tgcctggctg gtggtattca tggcgcgtat tctcttgcat tcgcccccgg
    10261 cgcgcaaacg gggcgatctg ccggccggcg gacttccgac tccgggaccg cgtggactag
    10321 acttcgtaga cttcgcgcct gccgcgccgt agccgccgcg gcgaaggcgg gacttcgcga
    10381 cttcgcgact tcgtggactt gatgtcgtcg tatgaacgat cgcgtgcgaa ctctggcggg
    10441 agttctctgg cagtatcacc agctcgggca cccgctcgag aaggcggatg cgatcctcgt
    10501 gctgtgcagc cacgacaagg cggtggccga acgtggcgcg cggctgttcc tggagcggtg
    10561 ggcgccgctg ctgatcttct cgggcggcct cggcgccatc acccggcacc tgtgggaccg
    10621 ccccgaggcc gaccagttcg ccgaggtcgc ccgcgcgatg ggcgtgccgg ccgatcggat
    10681 cctggtcgag aaccggtcca ccaacacggg cgagaacgtc gccttcaccc gccggctgct
    10741 cgaggagcgc ggcctcgacc ctggctcctt catcctcgtc cagaagccgt acatggagcg
    10801 gcgcagctac gccaccttcc agaaggtgtg gcccgggaag cgcctgatcg tcacctcgcc
    10861 gcgcgtctcg ctcgacgagt acctcgagcg gtactcgaac gagacgctct ctgccgatga
    10921 cgttgtgagc atcatggtgg gcgacctgca gcggattcgc ctctaccccg agaagggctt
    10981 ccaggtgccg caggagatcc cgccggaagt gtgggaggcc taccgcgagc tggtgtcgct
    11041 cggctacgac cggcacctcg ttaggggtta ggggctaggg gctaggggct aggggctagg
    11101 ggctcggggc tcggggcgct gggcggcctg ctggagccgc gctacactgc tcgcatgccg
    11161 accgcgacac ggaccccgct cttcaagccc cgcaccctct cgttccttcg cgccctcaag
    11221 cggaacaacg accgcgcgtg gttcgccgag cacaaggagg agtacctccg cgacgtgcac
    11281 cagccgatgc tcgacttcgt ggcgtgggtg gcctcggaac tgcccgcctt cgcgcccgag
    11341 ctcgaggcgt cgccgcggac ctcgctcttc cgcatctacc gcgacacgcg cttctccgcc
    11401 gacaagactc cgttcaagac gcaggtgggc gccatcttcc cgcaacgcgg cctgcacagg
    11461 aacgtgtcgg cgtgcctcta cgtggagatc gggcccgcag gcgccatgat cttcggcggc
    11521 atctacaagc cggagcgctc ggaactcctc gccatccgcg agcacatcgc ccgcacccac
    11581 cgccgccttc gcgcgctgct cgaggcgccg gcgttcaaac gcacggtggg cgcgatggag
    11641 ggcgacgcgc tgcagcgggt gccgcccggc tacgcacccg accatcccgc cgccgagtac
    11701 ctcaagttcc ggcagttcct gctcggcaag gaatacccgg cggccttcgc cacctcgccg
    11761 gaattccccg cggagatcct ccgtctcttc cgccgcatgg ctcacgtcgt cggctacctg
    11821 aacgagccgc tcctggccgc agaacgcgcg cgggatccgc tggccgagcg cagcccgcgc
    11881 cgcgagccga agcgcgtgag ccggctgatt ggctgagcga cgcgcctcga aaccgtgctc
    11941 tcagcgcccc ggggggccac gcggccggcc agcgtacctg ccgcttcagc ggttgcggag
    12001 gtggtcgagc gtgagccgaa tcccttcgtc gtacggcgtc tttcgcagcg gcagcacgga
    12061 ccctagcgcc gagtcgtcca gcagcaccgg cgtggtttgc aggtaggaca tccggacgcc
    12121 gtgcccgcgc cgcaggagct ccgccgccgc cgcacggccg atcgtcccga acgcaccgaa
    12181 gatcgcgacc ctcatggcca catggtgaca ggctcatggg tcgagctcaa gaacggttgc
    12241 gcgtcccacc agtgggatcc cacgatccca cagccggaca cccgcggatc gctcgtgcct
    12301 gccacgccgt accgccggag gatctcagac ggcgcgaagg cgggagcctt gagccgtgag
    12361 ccgtgagcct tcttccggca cgtttcttgg gttctccctc ccgggaggtc ccgtgcagga
    12421 gattcgacaa gccatgcggg cgctgtcgtc gcgcccgtcc ctggtagtgg tgtcggtgct
    12481 caccctggcc cttggaattg gcggcacgac cgcggtcttc tcggtcgtgg acgccgtcct
    12541 cctgaggccg cttcccttct ccggaagcga ccgcctggtg tcgctcaccg agagcagccc
    12601 gaacctgaag accctctccg tctcgtggca gaactacgtg gactggagac accagagccg
    12661 ctcgttcgag tcggtggcgg catacaggcc gatcaacgtc accatcaccg gggagggcga
    12721 gcccgagcga gtgccggcca agatggtttc ggcggcgatg ctgccgatgc ttggagtcaa
    12781 gccgtcgctc ggccgcagct tcaccaagga ggaagacggc gccggcgggg agccggtcgc
    12841 gctcatcagc gaggggctgt ggacccgccg cttcgcgcgc gatccgcggg ccgtgggcca
    12901 ggtaatgacc atcgacaaca cggcgcgcac gatcgtcggg gtcctgcccg ccagcttcgt
    12961 cctcgtgcag cctgccgacg tgctgatccc catcggtccc tgggcatcga cgctgcccga
    13021 cgaccgcggc tggcacccgg gcatcttccc gctggcgcgg ttgaagaacg gggtggaact
    13081 gggcaaggcc cgcctggaga tggcgcagat cgcgaagaac ctcgagcggc agtaccccga
    13141 aacgaacctg aaggtggggg ccgacgtcac gccgctgcag cagcagatgg tgcagaacgt
    13201 ccagccggcg cttgccgtac tgcttggcgc ggtcgtcctc gtcctgctga tcgcctgcgc
    13261 gaacgtggcg aacctgctgc tggtgcgcac catcgagcgg cgccgcgaga ttgcggtgcg
    13321 cgctgcgctc ggcgcccgcc gctggcggct gatccggcac gtgctggccg agagccttgt
    13381 gctggcgctg gccggaggtc tgctcggcgt gctggtcgcg tcctgggccg tctctgccct
    13441 ggtggcgctg gccgggcccg gtcttcccag ggccgacagc atcgcggtgg accagcgggt
    13501 gctgttgttc gcgctggcgc tctcgaccct cacggccctg gcatttggcg ccgccccggc
    13561 cgtccacgct tcccgggccg acatcaggga agcgttgaac gaggaaggcc gcgggacgac
    13621 gtcggccgga agccgccgcc tggcgcgtgc cttcgtcgtc gcggaggtgg cgctggccac
    13681 cacgctgctg gtgggcgccg gccttctgct ccgcagcttc gtgaggctgc agcaggtgcc
    13741 gcccggcttc agcgccgcgc gtctcctggt ggccgacctg ccgctttcgc ccatcgacta
    13801 cgccgaggac gcggcgcgcg agcggttcgt cgatcgcctg ctcgagcgcg tcacggccct
    13861 gccgggcgtc gaggcggccg ggacgacgac gacgctcccg atggcgggca gcggcgcgct
    13921 gctgcacttc aacatctacg ggcgtccgcc aaagggcccc gaggactaca tcctggcggg
    13981 gtaccgcgcg gtgagcccca cgtaccttgc agcgctcggc gtccctaccc ttcgcggccg
    14041 ccccttcacc gagtgggacc gctcggggtc gaagcgtgtc gtggtcatca acgagacgat
    14101 ggcgaagacc ttcttcaagg gaagcgaccc gctgggtcag cgcatcagca tcgggaccga
    14161 gcccgacgag gagtcgccgt ggatggaagt ggttggcgtg gtgggcgacg tgcgtcagag
    14221 cttcgaggcc gacccgaagg cggagatgta cgtcccgtac ttgcagggtg cgccccaccc
    14281 cgtcctcgcg ggtctcttcc gcagcgtctc ggtggtggtc cgcgccaagg gggatccact
    14341 ccaggtcgcc
//
LOCUS       DAOPQX010000056        14171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5033_length_14171_cov_4.366464, whole genome shotgun sequence.
ACCESSION   DAOPQX010000056 DAOPQX010000000
VERSION     DAOPQX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5033_length_14171_cov_4.366464"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKK95_04845"
     CDS             <1..517
                     /locus_tag="PKK95_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOC17572.1"
                     /translation="HSYRVSMYAVRIASRVRMSPAEVEDVRAAALLHDIGKLKIGREL
                     LHKAARLTAEEYERMQAHVPLGVEMLESVGGSLRRVLPLVLAHHERHDGNGQNHLSGD
                     QIPWGARVIKVADVYDSLTSDRPYRKAMPPFEARAHIAKGAGTEFDPYVVRAFIEAFE
                     AGDLELPQIVI"
     gene            complement(540..4025)
                     /locus_tag="PKK95_04850"
     CDS             complement(540..4025)
                     /locus_tag="PKK95_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC17573.1"
                     /translation="MTVDARRARCAPVTAVCLLALLAWLDPRGVERPEAAARGPAVED
                     AGPISHALAVSIDPSRHRLSATDVVRLPGRPPAGRVEFLLNGALSITSSEPPVREVPL
                     GDTSPFHGLNTGPGEDVPPLKRYSAVLPEGDAVLSLAYEGAVDFALSPEKEQYTRGFR
                     QTAGTIGAEGVYLAGAGFWYPQFGPGLISFTLEVSQPDGWHAVSQGNGTSRDAGGTAR
                     WDSAGPTDEIYLVGGPLRVFRDRAGPVETLVYLHEPDQGLAAKYLEATGQYIDMYRRL
                     IGPYPYGKFALVENFWETGYGMPSFTLLGREVIRFPFILASSYPHEILHNWWGNSVFV
                     DFASGNWSEGLTAYLADHLIQEQRGAGAEYRRATLQKYRNYVRDGRDFPLTEFRARES
                     AATEAVGYGKTLMAFHMLRRSVGDDVFRKALARFYRDFKGRRASFDDLRRTFESESGR
                     DLARFFRDWIERAGAPALAVTARRMRVDQAAAAAAPASAAAGWSVAGTLRQTQGGPAF
                     AVDVPIAIRTASGVERRVVRMAGAEQTFAFETASEPLALHADPDFDVFRRLDPRETPA
                     SIGQIFGEPHVLAIVPASAPEREQRAWRELFEGWGSPSHKVEVRTDREVASLPPDRAV
                     WIAGRGNALAGRVLESAGLGQSGGFVELDGQRIPLAAHTLVVVARHPATAEKAVGWLA
                     VDPIEAFPGLGRKLPHYGKYSYLAFEGAEPANTVKGQWTPADSPMTVRLRDPGGSSEA
                     GSARLAALEPERRKPLIELPPAFSERSLEAHVAFLADPARGGRMPGTGGHDEAARYIA
                     ERFKDIGLAPGGDEGGYLQEFTLPGGPAWKSAGGTRAAASNVIGYIPGTRADWKGQSV
                     VVSAHYDHLGAGWPDVHKGDEGKVHPGADDNASGVAVMLELARLLASSSPARSIVFIA
                     FSGEEAGRLGSRHYVERAAAFPARQAIGVINLDTVGRLGPRPVSVLGTATASEWQHIF
                     RGASFVTGVESLNVPAPPEASDQASFTALGVPGVHLFAGAHADYHRPTDTADKIDLAG
                     LVKVAALAREAIAYLAERAEPLTAPADSAASRPPAAGERTGGGERRAGVGTVPDFAFE
                     GPGVRVASVVAGSPAERAGIQAGDVLTAIDGREVADLRGYSELLRALKPGQKVRVVLV
                     RGGKEQALEVILGER"
     gene            4326..5612
                     /locus_tag="PKK95_04855"
     CDS             4326..5612
                     /locus_tag="PKK95_04855"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOC17574.1"
                     /translation="MDKKAFNPFEMAQAQFDKVADIINLDAPTRELLRNPLREYSFAI
                     PVRMDDGSVKVFRGFRVQHNDARGPAKGGIRFHPHETLDTVRALATWMTWKCAVVDIP
                     LGGGKGGVICDPHNLSQREQQGICRGWVRQLAKNVGPVNDVPAPDVMTNPQHMLWMLD
                     EFEAIHGGRYPGFITGKPVGMGGSLGRTEATGYGVIYTVREALKELGIEIAKTTAAMQ
                     GFGNVSQYAVRLFRQLGGKVVCVSSWDQADQTSYTFKKTAGVDPDELMGITDSFGGIN
                     KDKARKLGYEVLPGGAWIEQEADILVPAALENQVTAETVQKIHGKVRLIAEGANGPTT
                     PEADKVIKDRNIFVVPDFLANAGGVTCSYFEQVQGNMNYFWDRDEVLAKLDSKMTHAF
                     KAVSELARKQHLYMRDAAYVISVNRVAKACRDRGWV"
     gene            complement(5716..6327)
                     /gene="leuD"
                     /locus_tag="PKK95_04860"
     CDS             complement(5716..6327)
                     /gene="leuD"
                     /locus_tag="PKK95_04860"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOC17575.1"
                     /translation="MTRAQASGGGGRRASGRVVRVEGPAVALPRDNIDTDQIIPARFL
                     KTTTREGLGAHLFAGWRPCILDEPAARGARILVAGRNFGCGSSREHAPWALLDFGFGA
                     VVAGSFADIFRQNALKNGLLPVALPEPEHAQVLALTVSQPGVGLEVDLERQLLSWPSG
                     ACPFSIDPFARECLLQGVDEIGYVLRQEKAIEEYEARQPASER"
     gene            complement(6324..7724)
                     /gene="leuC"
                     /locus_tag="PKK95_04865"
     CDS             complement(6324..7724)
                     /gene="leuC"
                     /locus_tag="PKK95_04865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF004016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOC17576.1"
                     /translation="MPRTIIDKIWERHLVASPGAAPALLYIDLHLVHEVTSPQAFEGL
                     RRRGLRVRRPERTFAVADHAIPTTPLADPIADPVAARQVSQLEANCREFGIPCYGRGH
                     ERQGIVHVAGPELGLTRPGTTIVCGDSHTATHGAFGALAFGIGTSEVEMVLATQCLLQ
                     RRPRTCRIRVDGALAPGVSAKDLVLAIIAKLGVNGGTGFVFEYAGAAVRSLSMEGRMT
                     LCNMSIEAGARAGLVAPDDTTFAWLHGRPFAPSGAAWDAALRRWRELASDPDAAFDRE
                     ASLSADGLAPMITFGTNPAMAVAIDGSVPDPGGVSDEGDRQALRRALEYMAIEAGKPL
                     LGQRLDVVFVGSCTNGRLEDLREAARLLRGRRVHPGVRMLVVPGSRAVRAAAEAEGLN
                     RVFLDAGAEWREPGCSMCIAMNGDELKPGQRAISTSNRNFEGRQGPGSRTMLASPATA
                     AACAVAGVVADPRRFA"
     gene            complement(7772..8872)
                     /gene="leuB"
                     /locus_tag="PKK95_04870"
     CDS             complement(7772..8872)
                     /gene="leuB"
                     /locus_tag="PKK95_04870"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOC17577.1"
                     /translation="MLLRVLVLPGDGVGPEVTSEAVAVLERAAAVFGHQLETSCAAIG
                     GAAIEAEGVPLPEATARAAEEAPATLLGAVGLPRFDHLPPERRPERGLLDLRRRLGVF
                     ANLRPSRSWPSLVGASPLKNAVVEGTDLVFVRELTSGLYYGTPRGIEGAGGDERSVNT
                     MTYTRAEIERVARVAFELARGRRRRVTSVDKSNVLETSQLWRRVVTEVARDYPDVELG
                     HMFVDNCAMQLVLNPRQFDVVLTENLFGDILSDEAAVLAGSIGMLASASIGPLVGAAP
                     GEARARRGLYEPVHGSAPDLAGKGIANPLGAIGSAAALLQHSFGLHREAAAVTRAIDQ
                     VVASGRVTADLGPAGPPATTREVGQAVCEGIH"
     gene            complement(8874..10112)
                     /locus_tag="PKK95_04875"
     CDS             complement(8874..10112)
                     /locus_tag="PKK95_04875"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOC17578.1"
                     /translation="MPDRVTIFDTTLRDGEQSPGCSMTVPEKVRMAAALADLGVDVIE
                     AGFPIASEGDFEAVREVARTVRGVKVAALARACAADIARAAAALEGADRPVIHTFIAT
                     SDIHLRHKLRKPREQVLHEAAEAVRLARRHVDEVEFSAEDATRSEVDYLCAVAAEVVA
                     AGARTVNVPDTVGYCVPDEFSRIVARVVTEVGGAATVSVHCHNDLGLAVANSLAAVQA
                     GARQVECTINGIGERAGNCALEEIVMVLRVRADRMPFDTNVDTPRLYPASRLLSSLIA
                     FGPQPNKAIVGRNAFAHEAGIHQDGVLKERTTYEIMDPASVGVPESRLVLGKHSGRHA
                     LRKRCQDLGFALDAAALDAVYRRFIALADRKKGVTDEEIARLARIETGREVEAAPPAN
                     AGPGVVFPWPAATARAGRGF"
     gene            10302..11174
                     /locus_tag="PKK95_04880"
     CDS             10302..11174
                     /locus_tag="PKK95_04880"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOC17579.1"
                     /translation="MEWYSLQVFRAVATERSFSRAAAKLYRTQPAVSLAVQRLEEELG
                     EKLIDRSGKGLVLTDAGQVVLDHARRFDNLRTSMETALRELRDNAAGRLAIGANESTA
                     LYLLPHVTAYRKLYPHVTVQVRRSLSSRLPSQLVDGDIELGVISYDPHDPRLASTVIF
                     RDHLAFIVSPRHRLAARRSVSIRELGMENFIAHNVISPYRALVLEAFQRHKVPLHMDV
                     EMPTIETIRKLVQAGEGVAFLPRMCVDAEIERRALCEVPVRELKLTRQVRVVYPARRA
                     LSHASRAFLALVKA"
     gene            11193..>14171
                     /locus_tag="PKK95_04885"
     CDS             11193..>14171
                     /locus_tag="PKK95_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOC17580.1"
                     /translation="MPDILEQLQERAKELRALYRVHEAIRREGASLDEVCREVARVLP
                     EGWQYPSVAWCRVALEGTVHEPPGAVESPWGQRAEILVRGERAGSVEVYYTRQMPSAD
                     EGPFLREERKLLEAVADRLARFAERRRAGSAPAASPRGDDLTEEREWWLIVEFLRSTD
                     QHLLVRLARRLLNHLCWSGIAEAQDLLQRFAADTVATGQPEAGLEDNIPKRRKSLGEL
                     LDLTDATFEIAASHLGEREIVSCIENWIKDDRSGFLVEALENQCTSLSDIGSALERYH
                     QLALRDGELSRAVQIGLRVSLARRFFTDDLEFLNTAKNYIGIADFYELIHHIISPPAS
                     HGKLGGKSSGMLLASQILRKAADDELVSDVKVPKTWYIASDGLQSFAEHNQLEDVYNR
                     KYLEIDQVRREYPHIVQVFKNSSFAPDILAGLSKALDDLEGRPLIVRSSSLLEDRMGS
                     AFSGKYKSLFLANVGTKAERLGALTDAIAEVYASLFGPDPIEYRAERNLLDLHEEMGV
                     MIQEVVGSRVGPYYMPAFAGVAFSHNEFRWSPRIRREDGLVRLVPGLGTRAVDRVSDD
                     YPVLVAPGQSGLRVNVTREEILRYQPRRADVINLETRRFETVDIPDLLRAHARDYPGL
                     ALVASVLDPDGMIRPLGFLDDPARANLVVTFEGLIGGTPFMPRMRAILARLREKMGVP
                     VDIEFACDGRDFYLLQCRPQSFSLDSTAAALPRDVSPEHLLFDANRYVSNGRVPDITH
                     IVYVDPAAYDRLARLDDLRDVGRAVGRLNKLLPRRQFVLMGPGRWGSRGDIKLGVSVT
                     YSDINNAAVLIEIAARRGGSVPDLSFGTHFFQDLVEAGIRYLPLFPDEAGQALNEGFL
                     RGARNALAEVLPEFRHLAHVVHVVDVAQATGGLVLRVLMNADQERAMGVFGPPAFAQA
                     PPAPLERRPATPSLVTDDHWRWRLRMAERIAEQADAERFGIRGMYVFGSTKNATAGPE
                     SDINLIVHVTDGAPGRRELEA"
BASE COUNT         2067 a         5173 c         4959 g         1972 t
ORIGIN      
        1 ccacagctac cgcgtgtcga tgtacgcggt gcggattgcc tcgcgcgtga ggatgtcgcc
       61 ggccgaggtc gaggatgtcc gggccgcggc gctgctgcac gacatcggga agctgaagat
      121 cggacgcgag ctgctccaca aggccgcccg cctgaccgcg gaggagtacg agcggatgca
      181 ggcgcacgtg ccgctcggtg tcgagatgct ggaatcggtg gggggatcgc tgcggcgcgt
      241 gctgccgctc gtcctcgccc accacgagcg ccacgacggg aacgggcaga accacctctc
      301 gggcgaccag atcccctggg gagcccgtgt gatcaaggtg gccgacgtct atgattcgct
      361 gaccagcgat cgcccctacc gcaaggccat gcccccgttc gaggcccgcg cgcacatcgc
      421 caagggcgcc ggaaccgagt tcgaccccta cgtcgtccgc gccttcatcg aggcgttcga
      481 ggcaggggac ctcgagctgc cgcaaatcgt gatctagccg aacggtacgg cgaggggcac
      541 tacctctcgc ccaggatgac ctccagcgcc tgctccttgc cgccgcgcac cagcacgacg
      601 cgcaccttct gcccgggctt cagcgcgcgc agcaactccg agtagccgcg caggtcggcg
      661 acctcacgcc cgtcgatggc cgtcagcacg tcgcccgcct ggatgcccgc ccgttcggca
      721 ggcgacccgg caaccacgct cgccacgcga actcccggcc cctcgaacgc gaagtcgggc
      781 accgtgccga cgccggcgcg gcgctcgccg ccgcccgtcc gctcgccggc ggccggcggc
      841 cggctggccg cggaatcggc cggtgccgtc agcggctcgg cacgctcggc gaggtacgcg
      901 atcgcctcgc gggccagggc cgcaaccttc accaggccgg cgaggtcgat cttgtccgcc
      961 gtgtcggtcg gacggtgata gtcggcgtgc gccccggcga acaggtgcac gcccggaacg
     1021 cccagggccg tgaagcttgc ctggtcggac gcctcgggcg gggcggggac gttgaggctc
     1081 tcgacaccgg tgacgaagct cgcgccgcgg aagatgtgct gccactcgga agcggttgcg
     1141 gtgcccagca ccgacaccgg ccgcggcccg aggcggccca ccgtgtcgag gttgatcacg
     1201 ccgatggcct gccgcgccgg gaacgccgcc gcccgctcga cgtagtggcg cgaccccagc
     1261 cttcccgcct cctcgcctga gaaggcgatg aagacgatcg atcgcgccgg cgaggaggac
     1321 gccagcagcc gcgccagctc gagcatcacc gccacgccgc tcgcgttgtc gtcggcaccg
     1381 gggtgcacct tcccctcgtc tcccttgtgg acgtcgggcc acccggcgcc caggtggtcg
     1441 tagtgggccg acacgaccac cgattgcccc ttccagtcgg cgcgcgttcc cgggatgtag
     1501 ccgatcacgt tggaggccgc cgcgcgcgtg ccgccggccg acttccatgc agggccgccg
     1561 ggcaaagtga actcctggag gtacccgccc tcgtcgccgc ccggggcaag cccgatgtcc
     1621 ttgaaccgct cggcgatgta gcgcgccgcc tcgtcgtggc cccccgtgcc cggcatccgt
     1681 ccgccgcgcg cgggatcggc gaggaacgcc acgtgcgcct cgagcgatcg ctccgagaag
     1741 gccggcggca gctcgatcag cggcttccgc cgctcgggtt cgagcgcggc aagtcttgct
     1801 gagcctgcct cactcgaccc ccccggatcg cgaagccgga ccgtcatcgg ggagtcggcc
     1861 ggcgtccact gccctttcac ggtgttggcc ggctcggcgc cctcgaacgc caggtacgag
     1921 tacttcccgt agtgcgggag cttgcgcccc aaccccggga acgcctcgat cggatccacg
     1981 gccagccacc ccaccgcctt ctcggcggtc gcggggtgcc tggcgacgac caccagcgtg
     2041 tgcgcggcca ggggaatgcg ctgcccgtca agctcgacga agccgccgga ctggccgagg
     2101 ccggccgact cgaggacgcg acccgcgagc gcgttgccgc gccccgcaat ccagacggcg
     2161 cggtcgggcg gcagcgacgc cacctcccgg tcggtgcgca cctccacctt gtggctcgga
     2221 gatccccagc cctcgaagag ctcgcgccac gcgcgctgct cgcgctcggg ggcggaggcg
     2281 ggcacgatcg ccaggacgtg cggctcgccg aagatctggc cgatcgacgc cggggtctcg
     2341 cgagggtcga gccgccggaa gacgtcgaag tcgggatcgg cgtggagcgc cagcggctcg
     2401 gatgccgttt cgaaggcgaa ggtctgctcg gcccctgcca tccgcacgac gcgccgctcg
     2461 acgccgctcg ccgtccggat ggcgatgggg acgtccaccg cgaaagccgg cccgccctgc
     2521 gtctgccgca gcgtgccggc gacgctccag ccagcggccg cgctcgccgg cgcggcagcg
     2581 gccgcggcct ggtccacgcg catccgccgc gcggtcaccg ccagcgcggg cgctccggcc
     2641 cgctcgatcc agtcgcggaa gaacctggcg agatcgcggc cgctctccga ctcgaacgtc
     2701 cggcggaggt cgtcgaagga cgcccgcctt cccttgaagt cgcggtagaa gcgcgcgagc
     2761 gccttccgga agacgtcgtc gccgacggat cggcgcagca tgtggaaggc catgagcgtc
     2821 ttcccgtatc cgacggcctc ggtggccgcg ctctcgcggg cgcggaactc cgtcaatgga
     2881 aagtcgcggc catcccgcac gtagttccgg tacttctgca gcgtcgcgcg gcggtactcc
     2941 gcccccgcgc cgcgctgctc ctggatcagg tgatcggcca ggtaggccgt cagcccctcg
     3001 ctccagttcc cggaggcgaa gtcgacgaag accgagttgc cccaccagtt gtgcaggatc
     3061 tcgtgcgggt aagacgaggc gaggatgaag gggaaccgga tgacctcgcg gccgagcagc
     3121 gtgaagctcg gcatgccgta gccggtctcc cagaagtttt cgaccagcgc gaacttgccg
     3181 tacgggtagg gaccgatcag ccggcggtac atgtcgatgt actgaccggt tgcctcgagg
     3241 tacttcgcgg cgaggccctg gtcgggctcg tgcaggtaga cgagcgtctc gaccgggccg
     3301 gcacgatcgc ggaagacccg cagcgggccg ccaaccaggt agatctcgtc ggtcggccct
     3361 gccgagtccc atcgcgcggt cccgccggcg tcacgcgagg tgccgttccc ctgcgagacg
     3421 gcgtgccagc cgtcgggctg cgatacctcc agcgtgaagg aaatcaatcc aggtccgaac
     3481 tgcggatacc agaacccggc gcccgcgaga tagacccctt cggcgccgat ggtgccggcg
     3541 gtctgcctga acccgcgggt gtactgctcc ttctcgggcg acagggcaaa gtcgacggcc
     3601 ccctcgtagg cgagcgacag cacggcgtcg ccctccggga ggacggcgct gtaccgcttc
     3661 agcggcggta cgtcctcgcc cggaccggtg ttgagaccgt gaaagggcga ggtgtcgccg
     3721 agcggcacct cgcgcaccgg cggctcgctg ctcgtgatgg agagggcgcc gttcagcagg
     3781 aactcgacgc ggccggccgg cggccgcccg ggcaaacgca ccacgtcggt cgcgctcagg
     3841 cggtgccgcg acggatcgat cgacacggcg agggcgtgag agatgggtcc cgcgtcctcg
     3901 acggccgggc cgcgcgcggc cgcttcggga cgttcaacgc cacgcgggtc gagccacgcg
     3961 agaagggcga gcaggcaaac ggcggtcacc ggggcgcatc gcgcgcgccg ggcatctaca
     4021 gtcatcaaat tctccaggtc gagcaatggc ccaggagaat tgtacggtac cagcggccgc
     4081 cgcttccttc tccggcgttt cctccgcctc cttgacttca acaggctccc tcaatacgct
     4141 ggcagacggt ctacggccgg ggacgggccg catggaagcg ccgtctcggc caggtccctg
     4201 cccgtttgta acactcctga ccggcatgct cgcccttgcc cccttcggtc aggcgtggtg
     4261 tggcaccacg gcactattcg cgtctctttc gaggccggcc tgacagtaaa agagggggag
     4321 catcagtgga caagaaggcg ttcaacccgt tcgagatggc ccaggcgcag ttcgacaagg
     4381 tggccgacat catcaatctc gatgcgccca cccgggagct gctgcggaac ccgctccgcg
     4441 agtactcatt cgccatccct gtccgcatgg acgacgggtc ggtgaaggtg ttccggggct
     4501 tccgcgtcca gcacaacgac gcgcgcgggc cggccaaggg cggcatccgc ttccacccgc
     4561 acgagacgct cgacaccgtg cgcgccctgg cgacgtggat gacctggaag tgcgcggtcg
     4621 tggacatccc cctcggcggc ggcaagggcg gcgtcatctg cgacccccac aacttaagcc
     4681 agcgcgaaca gcagggcatc tgccggggct gggtgcgcca gctcgccaag aacgtcgggc
     4741 cggtcaacga cgtcccggcg cccgacgtga tgaccaaccc gcagcacatg ctgtggatgc
     4801 tcgacgagtt cgaggcgatc cacggcggcc gctacccggg cttcatcacg ggcaagccgg
     4861 tgggcatggg cggatcgctc ggccgcacgg aggcaaccgg ctacggcgtc atctacaccg
     4921 tgcgcgaggc gctgaaggag ctgggcatcg agatcgccaa gacgaccgcg gcgatgcagg
     4981 gcttcgggaa cgtgtcgcag tacgctgtcc gcctgttccg gcagctcggg ggcaaggtgg
     5041 tctgcgtgtc gtcgtgggac caggccgatc agacctcgta caccttcaag aagactgccg
     5101 gcgtcgatcc cgacgagctg atggggatca ccgacagctt cggcggcatc aacaaggaca
     5161 aggcgcgtaa gctcgggtac gaggtgctgc ccggcggcgc ctggatcgag caggaggccg
     5221 acatcctcgt cccggcggcc ctcgagaacc aggtcaccgc cgagacggtg cagaaaatcc
     5281 acggcaaggt gaggctgatt gccgagggcg ccaacggtcc gacgaccccc gaggccgaca
     5341 aggtcatcaa ggaccggaac atcttcgtcg tccccgactt cctcgccaac gccggcggcg
     5401 tgacctgcag ctacttcgag caggtgcagg gaaacatgaa ctacttctgg gatcgcgacg
     5461 aggtgctcgc gaagctcgac tccaagatga cccacgcctt caaggcggtg agcgagctgg
     5521 cgcgcaagca gcacctctac atgcgcgacg cggcgtacgt gatctcggtc aaccgcgtgg
     5581 cgaaggcgtg ccgcgatcgg ggctgggtct aggaaagagg cgacagatag aggaagggcg
     5641 gcctcgccag atcgaggccg cctttttttt cgcgctgccg ggccccgcgg gctccccgcg
     5701 agccgccgcc ggcggtcagc gttctgacgc cggctgcctg gcctcgtatt cctcgatcgc
     5761 cttctcctgg cgcagcacgt agccgatctc gtccaccccc tgcagcaggc actcccgcgc
     5821 gaacgggtcg atcgagaacg ggcacgcgcc ggaaggccag gagagcagct gccgctcgag
     5881 gtccacctcc aggcccacgc ctggctgcga caccgtcagc gcgagcacct gggcgtgctc
     5941 gggctcgggc agggccaccg gcagcaggcc gttcttcagc gcgttctgcc ggaagatgtc
     6001 cgcgaacgag cctgcgacca ccgcgccgaa cccgaagtcg agcagcgccc acggcgcgtg
     6061 ctcgcgcgag ctgccgcagc cgaagttgcg gcccgccacc aggatgcgtg cgccgcgtgc
     6121 cgccggctcg tcaaggatgc aggggcgcca cccagcgaac aggtgcgcgc caagcccctc
     6181 cctcgtcgtc gtcttcagga accgggcggg gatgatctga tcggtgtcga tgttgtcgcg
     6241 gggcaaggcc accgccgggc cctccacacg cacgacgcgc ccggacgccc tccgtccccc
     6301 gcctccgctt gcctgcgcgc gcgtcatgcg aacctccgcg gatcggccac cacgccggcc
     6361 acggcgcagg ccgcggcggt cgcggggctg gccagcatcg tccggcttcc gggaccctgc
     6421 cgcccctcga agttgcggtt gctcgtcgag attgcgcgct ggcccggctt cagctcgtcg
     6481 ccgttcatcg cgatgcacat cgagcacccc ggctcgcgcc actcggcacc cgcgtcgagg
     6541 aagacgcggt tcagcccctc cgcctcggcg gccgcgcgca cggccctcga gcccggcacc
     6601 accagcatcc ggacgccggg atggacgcgc cggccccgca ggaggcgcgc cgcctcccgc
     6661 aggtcctcga gacggccgtt ggtgcagctc cccacgaaaa cgacgtcgag ccgctggccg
     6721 agaagcggct tccccgcctc gatggccatg tactcgagcg cgcggcgcag ggcctgccgg
     6781 tctccttcgt cggacactcc gcccgggtcg ggcacgctgc cgtcaatcgc aaccgccatc
     6841 gccgggttcg tgccgaacgt gatcatcggc gcgagcccat cggccgacag gctcgcttca
     6901 cggtcgaagg ccgcgtccgg gtcgctcgcc aactccctcc accgccgcag ggcggcgtcc
     6961 cacgcggccc ccgacggcgc gaacggccgg ccgtgcagcc aggcgaacgt ggtgtcgtcg
     7021 ggcgcgacga ggcccgcccg cgcgcccgcc tcgatcgaca tgttgcagag cgtcatccgc
     7081 ccttccatcg acagcgagcg cacggccgcc ccggcgtact cgaacacgaa gccggtgccg
     7141 ccgttgaccc ccagcttcgc aatgatcgcc agcacgaggt ccttggccga cacgcctggg
     7201 gcgagcgcgc cgtctacgcg gatccggcag gttcgcggac gccgctggag caggcactgg
     7261 gtggccagca ccatctccac ctcgctcgtc ccgatgccga acgcgagcgc gccgaacgcg
     7321 ccgtgcgtgg cggtgtggct gtcgccgcag acgatcgtgg tgccgggacg cgtcaggccc
     7381 agctcgggcc ccgccacgtg cacgatcccc tgccgctcgt ggccgcggcc gtagcacggg
     7441 atcccgaatt cgcggcagtt ggcctcgagc tgggagacct ggcgcgcggc cacggggtcg
     7501 gcgatcgggt cggccagcgg cgtggtaggg atggcgtggt cggccaccgc gaaggttcgt
     7561 tccgggcggc ggacgcgcag gccccggcgg cgcagcccct cgaacgcctg cggtgaggtc
     7621 acctcgtgga cgagatggag atcgatgtaa aggagtgccg gcgccgcccc gggcgaggca
     7681 acgaggtgac gctcccagat cttgtcgatg atggtgcgcg gcatggcggt ccgatccggg
     7741 caggcggagg ggggatccgc ctgggtaggc ctcagtgaat cccctcgcac accgcttgcc
     7801 cgacctcgcg ggtggtggcg ggcgggccgg ccggcccgag gtcggccgtg actcgccccg
     7861 aggcgacgac ctggtcgatg gcacgcgtga cggcagccgc ctcgcggtgg agcccgaagc
     7921 tgtgctggag caaggccgcc gccgatccga tggcgccgag cgggttggcg attcccttcc
     7981 ccgcgaggtc gggcgcggac ccgtggaccg gctcgtagag tccacgacgg gcccgcgcct
     8041 ccccgggagc cgcccccacc agcggcccga tggaggcgga cgcgagcatg ccgatcgacc
     8101 cggcgagcac ggccgcctca tcgctgagga tgtcgccgaa caggttctcg gtgaggacga
     8161 cgtcgaactg cctcgggttc agcacgagct gcatcgcgca gttgtccacg aacatgtgcc
     8221 cgagctccac gtcggggtag tcgcgggcga cctcggtgac gacgcgccgc cacagctgcg
     8281 aggtttcgag gacgttcgac ttgtccacgc tggtgacgcg gcgccgccgc ccgcgcgcca
     8341 gctcgaaggc cacgcgcgcc acccgctcga tctcggcgcg cgtgtaggtc atcgtgttga
     8401 cgctgcgctc gtcgccgccc gcgccctcga tgccgcgggg ggtgccgtag tagaggcccg
     8461 aggtcaactc gcgcacgaag accaggtcgg tcccctccac cacggcgttc ttcaacgggg
     8521 aggcccccac gagcgagggc cacgagcgcg acggccgcag gttggcgaac acgccgagac
     8581 ggcgccgcag gtcgagcagg ccgcgctcgg ggcgccgctc gggcgggagg tggtcgaaac
     8641 gcggcaggcc caccgcgccg agcagcgtgg ccggggcctc ctcggccgcg cgcgcggtcg
     8701 cctcgggcag cggcaccccc tcggcctcga tcgccgcgcc gccgatcgcc gcgcacgagg
     8761 tttccagctg gtggccgaac acggcggcgg cgcgctcgag caccgccacc gcctcggatg
     8821 tcacctcggg cccgacgccg tcacccggga gcacgaggac gcggaggagc acctcagaag
     8881 ccccgtcccg cgcgggcggt ggccgccggc caggggaaca cgacgccggg gccggcgttc
     8941 gcgggcgggg ccgcttcgac ctcccggccc gtctcgatcc gggccaggcg ggcgatctcc
     9001 tcgtcggtga cccccttctt ccgatcggca agcgcgatga agcggcggta gaccgcgtcg
     9061 agggcggcgg cgtcgagggc gaagccgagg tcctggcagc gcttgcgcag cgcgtggcgc
     9121 ccgctgtgct ttcccagcac gagccggctc tcgggcacgc cgaccgacgc ggggtccatg
     9181 atctcgtagg tcgtccgctc cttgaggacg ccgtcctggt ggatgccggc ctcgtgggcg
     9241 aaggcgttgc ggccgacgat cgccttgttc ggctggggcc cgaaggcgat gaggctgctc
     9301 agcaggcggc tggccgggta caggcgcggc gtgtcgacgt tggtgtcgaa gggcatgcgg
     9361 tcggcccgca cgcgcagcac catcacgatc tcctcgagcg cgcagttgcc ggcccgctcg
     9421 ccgatgccgt tgatcgtgca ctccacctgc cgcgcgcccg cctgcacggc cgcgagcgag
     9481 ttggccaccg ccagcccgag gtcgttgtgg cagtgcacgc tcaccgtggc cgccccgccc
     9541 acctcggtga ccacgcgcgc cacgatgcgg gagaactcgt cgggcacgca gtagccgacc
     9601 gtgtcgggga cgttgacggt gcgcgcgccg gcggcgacga cctcggcggc gaccgcgcac
     9661 aggtagtcga cctcgctccg cgtcgcgtcc tcggcggaga actccacctc gtcgacgtgg
     9721 cggcgcgcga gccggaccgc ctcggccgcc tcgtggagca cctgctcgcg gggcttgcgc
     9781 agcttgtggc ggaggtggat gtcgctggtg gcgatgaagg tgtggatcac ggggcggtcg
     9841 gccccctcga gggcggcggc ggcccgcgcg atgtcggcgg cgcaggcccg cgccagggcg
     9901 gccaccttca cgccgcggac cgtgcgcgcc acctcgcgga cggcctcgaa atccccctcc
     9961 gacgcgatcg ggaagccggc ctcgatcacg tccacgccga ggtcggcgag ggcggccgcc
    10021 atgcgcacct tctcgggcac ggtcatgctg cagccgggcg actgctcgcc gtcgcgcagg
    10081 gtggtgtcga agatggtcac gcggtcgggc atcgggtctc ctccgtcgac ccgtcactat
    10141 ccgcccgcac cctcgatttg gcaaatttat agttctgtga tgatctataa cctcgtcggc
    10201 cgcctataag cggcgcttat gcacccggcg gcgcgccggc cggcgcgggg cgggccctcc
    10261 cgccgcgcct tccgcccgcc gcccttctcg cggggtgggc catggagtgg tattcgctgc
    10321 aggtgttccg cgcggtggcg accgagcgca gcttctcgcg cgcggccgcc aagctgtacc
    10381 gcacgcagcc ggccgtgtcg ctcgccgtgc agcggctcga ggaggagctg ggcgagaagc
    10441 tcatcgaccg ctccggcaag ggcctcgtcc tcaccgacgc gggccaggtg gtgctcgatc
    10501 acgcccggcg cttcgacaac ctgcggacct ccatggaaac cgccctccgc gagctgcgcg
    10561 acaacgcggc gggccgcctg gccatcgggg cgaacgagtc caccgccctc tacctgctgc
    10621 cccacgtcac cgcgtaccgc aagctctacc cgcacgtcac ggtgcaggtg cgccgaagcc
    10681 tgtcgagccg cctgccgtcc cagctggtgg acggagacat cgagctgggc gtcatcagct
    10741 acgaccccca cgacccccgc ctcgcctcca ccgtcatctt ccgggatcac ctcgccttca
    10801 tcgtgtcgcc gcgccaccgc ctggccgcgc ggcgcagcgt ctcgatccgc gagctgggca
    10861 tggagaactt catcgcgcac aacgtcatct cgccgtaccg cgccctcgtc ctcgaggcct
    10921 tccagcgcca caaggtgccc ctccacatgg acgtcgagat gccgacgatc gagacgatcc
    10981 gcaagctcgt ccaggcgggc gagggggtgg cgttcctgcc caggatgtgc gtcgacgcgg
    11041 agatcgagcg gcgggcgctg tgcgaggtgc cggtccggga gctgaagctc acgcggcagg
    11101 tgcgcgtggt ctaccccgcc cggcgggcgc tgagccacgc ctcgcgcgcg ttcctcgcgc
    11161 tcgtcaaggc gtagtattgg tactcttcta ccatgcccga catcctcgag cagctccagg
    11221 agcgcgccaa ggaactgcgc gcgctgtacc gtgtccacga ggcgatccgg cgcgagggcg
    11281 cctcgctcga cgaggtgtgc cgcgaggtcg cccgggtcct gccggaggga tggcagtacc
    11341 cgagcgtcgc ctggtgccgg gtcgccctcg aggggaccgt gcacgagccg ccgggcgccg
    11401 tcgagagccc ctgggggcag cgcgccgaga tcctcgtccg cggcgagcgg gccggctcgg
    11461 tggaggtcta ctacacgcgt caaatgccgt ctgcagacga ggggccgttc ctcagggagg
    11521 agcggaagct gctcgaggcg gtggccgacc ggctggcccg cttcgcggag cggaggcggg
    11581 cgggctcggc tcccgccgcg tcgccccgcg gcgacgacct gaccgaggag cgggagtggt
    11641 ggctgatcgt cgagttcctc cggagcaccg accagcacct gctcgtgcgc ctggcgcgcc
    11701 ggctgctcaa ccacctctgc tggagcggca tcgccgaggc gcaggacctg ctccagcggt
    11761 tcgcggccga caccgtcgcg acggggcagc cggaagccgg gctcgaggac aacatcccga
    11821 agcgccggaa gagcctcggc gagctgctcg acctgaccga cgccaccttc gagatcgccg
    11881 cctcccacct cggcgagcgc gagatcgtct cgtgcatcga gaactggatc aaggacgacc
    11941 gctcgggctt cctggtggag gcgctcgaga accagtgcac ctcgctcagc gacatcggca
    12001 gcgcgctcga gcgctaccac cagctggcgc tgcgcgacgg cgagctgtcg cgcgcggtgc
    12061 agatcgggct gcgggtgtcg ctcgcgcggc gcttcttcac cgacgacctc gagttcctca
    12121 acaccgccaa gaactacatc gggatcgccg acttctacga gctgatccac cacatcatca
    12181 gcccgccggc cagccacggg aagctggggg ggaagagctc ggggatgctg ctcgcctcgc
    12241 agatcctccg caaggcggcc gacgacgagc tggtgagcga cgtcaaggtg ccgaagacct
    12301 ggtacatcgc ctcggacggc ctccagagct tcgccgagca caaccagctg gaggacgtct
    12361 acaaccggaa gtacctcgag atcgaccagg tccggcgcga gtacccgcac atcgtccagg
    12421 tcttcaagaa ctccagcttc gcgcccgaca tcctcgccgg gctgtcgaag gcgctcgacg
    12481 acctcgaggg ccgcccgctc atcgtccgca gctccagcct gctcgaggac cggatggggt
    12541 cggccttctc ggggaagtac aagagcctct tcctcgccaa cgtcggcacc aaggcggagc
    12601 ggctcggcgc gctgaccgac gcgattgccg aggtgtacgc ctcgctgttc ggccccgacc
    12661 cgatcgagta ccgggccgag cgcaatctcc tcgacctcca cgaggagatg ggcgtgatga
    12721 tccaggaggt ggtcggctcg cgcgtgggcc cctactacat gccggcgttc gcgggagtcg
    12781 ccttcagcca caacgagttc cggtggtcgc cccgcatccg tcgcgaggac ggtctcgtgc
    12841 gcctggtgcc gggtctcggc acccgcgcgg tcgaccgcgt gagcgacgac tacccggtgc
    12901 tggtggcgcc cggccagtcg gggctccgcg tcaacgtcac cagggaggag atccttcgct
    12961 accagccgcg gcgcgccgac gtgatcaacc tcgagacgcg gcggttcgag acggtggaca
    13021 tccccgatct cctccgggcg catgcccgcg actaccccgg gttggcgctg gtcgcctcgg
    13081 tgctcgaccc ggacgggatg atccggcctc tcggcttcct cgacgacccc gcgcgcgcga
    13141 acctcgtggt caccttcgag gggctgattg gcggcacccc cttcatgccg cgcatgcgcg
    13201 cgatcctcgc gaggctgcgc gagaagatgg gcgtgccggt ggacatcgag ttcgcctgcg
    13261 acgggcggga cttctacctg ctgcagtgcc ggccccagag cttctcactc gactcgaccg
    13321 ccgcggcgct accgcgtgac gtctcgcccg agcatctcct gttcgacgcc aaccggtacg
    13381 tctcgaacgg ccgcgtcccc gacatcacgc acatcgtcta cgtcgatccg gccgcctacg
    13441 accggctcgc ccgcctcgac gacctcaggg acgtggggcg cgcggtcggg cggctgaaca
    13501 agctgctccc gcgccggcag ttcgtcctga tggggccggg gcgctggggc agccgcggag
    13561 acatcaagct cggggtcagc gtcacctact ccgacatcaa caacgccgcc gtgctgatcg
    13621 agatcgccgc ccgccggggc ggctcggtgc ccgacctctc gttcgggacg cacttcttcc
    13681 aggacctcgt cgaggccggc atccgctacc tgccgctctt ccccgacgag gccggacagg
    13741 cgctgaacga ggggttcctg cggggcgccc ggaacgccct cgccgaagtg ctgcccgagt
    13801 tccgccacct cgcccacgtc gtgcacgtcg tggacgtcgc gcaggccacc ggcggcctgg
    13861 tgctgcgggt gctgatgaac gccgaccagg agcgcgcgat gggcgtgttc ggcccccccg
    13921 ccttcgcgca ggcgccgccg gcgcccctcg agcgccgccc cgcgacgccg tcgctggtca
    13981 ccgacgacca ctggcgctgg cggctgcgca tggccgagcg gatcgccgaa caggccgacg
    14041 ccgagcggtt cggcatccgc gggatgtacg tgttcgggag cacgaagaac gccacggcgg
    14101 ggcccgagag cgacatcaac ctgatcgtgc acgtcaccga cggcgcgccg ggccggcgcg
    14161 agctcgaggc c
//
LOCUS       DAOPQX010000057        14167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5035_length_14167_cov_4.434666, whole genome shotgun sequence.
ACCESSION   DAOPQX010000057 DAOPQX010000000
VERSION     DAOPQX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14167
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5035_length_14167_cov_4.434666"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKK95_04890"
     CDS             complement(<1..533)
                     /locus_tag="PKK95_04890"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17581.1"
                     /translation="MTGFASAAREDESAAVGVTIRAVNHRYLDLQLRLPPGLAPIEGR
                     LRAAVQRQVARGRVELSVSVQLRQAPPPEVELNEPFVRRLTGALERAREEGLVDGALT
                     PGDLLRLPQALVIREAAKEDVEGVDAGTLAEAAVEEALAALNEMRAREGSHLQDDLEA
                     RRALLADLLEQAATAAER"
     gene            complement(601..2379)
                     /locus_tag="PKK95_04895"
     CDS             complement(601..2379)
                     /locus_tag="PKK95_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC17582.1"
                     /translation="MNALRRLLRFAAPYRGRLLAAFAWMVLYAAASACLAALIKWVFD
                     DVLERQANVGVVSSAIVGFYLLKGLGSYFSTYLMADVGQRVVRDVRQALYGHIIGQSA
                     SFFARRTTGQLMSRLTNDVSMIQQAVSETLGDLVRESLALVGYVALLFYLEARLAIVF
                     LTGAPLVVYPLVRLGQRARRTTRRSQEELERLSHLSAETFTGHRIVKAFGTEREEQRR
                     FAGAADRVYRANMKVTAVLSSLPPVMELLGALGIVAALWYGSREMTVGEFTTFMAALM
                     LMYAPAKKLSRVNATLQQTIAASERIFELLDTHNEILDRPGARPLAPLSRGIEFRDVG
                     FGYEDDRRHPILRGVSLSVAAGEKVAIVGRSGAGKTTLVNLLPRFHDVTSGAILIDGV
                     DIRDVTLASLRGQIGMVTQETILFDDTIARNIAYGSAGATDAEIEAAARAAYAHEFIR
                     AMPRGYQTVIGERGQRLSGGQRQRIAIARALLRNSPILILDEATSSLDSESEQLVQEA
                     LANLMANRTAIVIAHRLSTVQRADLIVVLDRGRVVETGRHEELLARPRGAYARLYAMQ
                     MAAGRRGGPEAGALHRETLGLPWGEG"
     gene            complement(2376..3119)
                     /locus_tag="PKK95_04900"
     CDS             complement(2376..3119)
                     /locus_tag="PKK95_04900"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC17583.1"
                     /translation="MKTEVVAATAPAAGLKGGLVDPLLSVVMPVYNERDTVEEIIRRV
                     HAVPLRTELIVVDDGSTDGTREILGRLQAELGFQLLLQERNQGKGAALRRGFSEVTGD
                     LVVIQDADLEYSPEEYPQLVELICSGRADVVYGSRFLGRHRVFMFSHYLGNRIVTLLT
                     NVLYNTMLTDMETCYKVMRAEIARGLDLKSNGFGIEPEMTAKIFKRGYRVYEIPITYD
                     GRGYEQGKKITWRDGLVALWVLLRYRFTD"
     gene            complement(3116..4144)
                     /gene="galE"
                     /locus_tag="PKK95_04905"
     CDS             complement(3116..4144)
                     /gene="galE"
                     /locus_tag="PKK95_04905"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOC17584.1"
                     /translation="MASRTTASGARGSAVLVTGGAGYIGSHAVKALCEAGRRVVVYDD
                     LSEGHRAAVPGVDLVEGDTGDLSRLREAIATRGVTAVMHFAAWTAVGESVAEPAGYYR
                     NNVGGTLAVLEAMAREKVRVFIFSSTAAVFGEPEQVPIDEHHPARPINPYGETKLVVE
                     RALPHYERAYGIRSVCLRYFNAAGADPAGTLGEDHHPERHLIPLALRAATGGTPLQVF
                     GSDYPTPDGTCLRDYVHVTDLAQAHLLALDGLEAGSPSTVYNLGNGRPYSVREVIAAV
                     ERVTGRPVPYALGPRRPGDPAVLYASSARIKAELGWKPRYEELDTIVRTAWAWLEAHP
                     NGYREVRA"
     gene            complement(4113..5408)
                     /locus_tag="PKK95_04910"
     CDS             complement(4113..5408)
                     /locus_tag="PKK95_04910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOC17585.1"
                     /translation="MKLSVIGAGYVGLVAAACFAENGNDVVAVDRDPARLRLLRRGAI
                     PFVEPGLEEIVRRNRAEKRLVFTSQVATAARHSSIVFIAVEAASSVDGEAAAQGVLDA
                     AREVAQAMNGYKVLAIASAVPVGTAAKAREVVRRETTHPFSVVSSPAFLRQGTAVRDF
                     LEPDRVVIGAEDPRATQLLVDLYSPFTRTGAPVLVMDCASAELSKLAASALLAARISL
                     MNEVARVAELAGADVEQVRRAVGADHRIGPACLFAGAGYGGANLAGDVRTLMALAAGL
                     GYGFRALEAAERVNAEQKEWLACRMQRHFGSLEGRVIAVWGLAFKPGTGDVREAPALA
                     LVERLLAAGARVQVYDPAASSTAKAALASRVKFCARSYDALVGADALGLVTEWNEFRE
                     PDLSKMRKLMRAPVVFDGRNLYRPERMRAHGFTYYGVGR"
     gene            5606..6757
                     /locus_tag="PKK95_04915"
     CDS             5606..6757
                     /locus_tag="PKK95_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOC17586.1"
                     /translation="MRLLLDYRPALRERTGVGEYVHQLARALVQLSEDGAAEGGLTVE
                     LFTSSWKDRPDPAAVAELGPVRVVDRRVPVRTLNWAWHRLRWPPAELLAGGRYDVVHS
                     PHPLLLPSRRAARVITIHDLDFLLHPERTREEIRRDYPAFVRRAAHTAELVIVPSAHV
                     GRVAAGLLELDASVVCVCPQGGPGWEPDPAQARGNPGGRYILFLGTLEPRKNVQGLLD
                     AYAALVTRWPQAPPLVLAGHGTPEAERWLEATLRPPLAGHVECRGYVPSHERRALYEG
                     ARLLVLPSFDEGFGLPVIEAMSAGIPVVASDRGALPEVCGGAALLVDPDDRGSICAGI
                     ERVLREAGLAGRLAERGLARARDFSWRRTAELTFEAYRRALVRRARGAA"
     gene            6757..7842
                     /locus_tag="PKK95_04920"
     CDS             6757..7842
                     /locus_tag="PKK95_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOC17587.1"
                     /translation="MRVAIDGRELCGRPTGVGRFLSHLLAAWAKLPEAREHEYLLYVP
                     EVPLRLPPDLRVHTRVLPGASGTWWEQVRLASAVRRDCPDVLFCPAYTSPLAAGVPVV
                     VALHDVSFLAHPEWFPAGSGLRRRLLARLSARRAVSVFTISEFSRGEIAFRLRVARER
                     VRVVPLAPGGPPAAPGLLREPLVLYVGSIFNRRHLPELIAACGKLALGHPEVRLEVVG
                     EDRTYPSQDLGAAAAASGMEGRLSLRSYVPDEELAVLYGKASVFAFLSDYEGFGLTPL
                     EAIAAGVPAVLAETAVAREVCGDAALYVPTGDVGAIAAALESALFDPATRRRLLAAGP
                     ATLSRYSWDRVGRDTLAVLLDAARRRA"
     gene            7844..8671
                     /locus_tag="PKK95_04925"
     CDS             7844..8671
                     /locus_tag="PKK95_04925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC17588.1"
                     /translation="MQADLAIVIVSYNARRDLERALSSLAVAPPRIAHEIVVVDNASS
                     DGSTAMVRERFPEVRVIEAGGNIGFARANNLGIRATSGDLILLLNSDTVVPPGAVDAL
                     VADLLAHPEVAVAGPRLVDGSGRAEWSFGEMMTPWAELRRKLAGRLYDARFPLVTRLV
                     ERRLAAAQAVDWVSGACLLVHRADADAVGLLDERFFLYTEDVDFCASIRAQGRQVRYT
                     PAAQVVHLRGRSRSHDPRGASAHYRRSQLAFYAKHHPAWHRVLRAYLRLKGQLPKDS"
     gene            8799..9929
                     /locus_tag="PKK95_04930"
     CDS             8799..9929
                     /locus_tag="PKK95_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOC17589.1"
                     /translation="MRIAIDARKYLDFGIGTYIRNIVRELARIDGETEYVLLCREADR
                     EAIATLLPKNFRPVVEPAPPYSVAEQVRVPIALRRERVDLFHAPHYVLPPLIHCRSVV
                     TIHDIIHLLFPQYLPGRLAHLYARASLWGAARRSDRILTVSETSKLDILRRFRVPADK
                     ITVVYNAIDVRLLAEPSPEEFARVRERYQLNHPFALYVGNIKPHKNLERLIEAFDRLR
                     REPGFEALRLLIIGDQISKYQSLRRAVHRHKLHKQVRFFGFVPLETLAVLYRLASVFV
                     FPSLYEGFGLPPVEAMACGLPVVTSNISSLPEVVGDGAILVDPYSPDSIAEGMRAALT
                     DEELRATLRERGLARAREFSWEQSVRRIREVYDEVMAQPYEP"
     gene            9919..11058
                     /locus_tag="PKK95_04935"
     CDS             9919..11058
                     /locus_tag="PKK95_04935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17590.1"
                     /translation="MSRDRRRGLPRIALVHDWLTGMRGGEKVLEALCELYPEAPVFTM
                     VHVPGTESRTIARHHPRGAWLSRLPGVGRYYRHLLPLFPLAAETFDLDEFDLVISSSH
                     CAVKSIVRPGRARHLSYCHTPMRYVWDQRDAYFGPERLGRAGSRALRPVLAWLARWDA
                     ATAGRVDRYLANSQHVARRIHRYYNRRAEVVAPPVDTGFFHPDGRLPGDFALAVSALA
                     PYKRLDVAIEGCRLAGIPLRIVGWGPEERRLRAQARGGAELLGTLSDEEIRELYRSCA
                     MVLLPGEEDFGIVPVEAQACGRPVVAAAAGGALETVVDGTTGVLVAPGSAEALAEGIR
                     RVRATRFDEAVLRRHAEAFGRERFKARISAIVDETLASGASPTTW"
     gene            11052..12488
                     /locus_tag="PKK95_04940"
     CDS             11052..12488
                     /locus_tag="PKK95_04940"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HOC17591.1"
                     /translation="MVKRYNRLLAAVHVLSDAILASAAFMLAYLLRFEAGLVPVTKGY
                     PPLSQYLRILPVVAILVPFGYHLQGLYRLRRGRSRVDDFFAVFVGTVLAVIFGIVATL
                     YFQAYYASPALKEVGAFQVSQIVWAVFLVANIALTFGSREMMRQLLERRWRAGIGLKR
                     VLIAGAGDLGRVVVDRILDHRELGFQIVGFVDDRAGGDHLGHRGLPLLGTLREVSEIV
                     QRERVDHVYVTLPLEEHGKIVQVIEGAAREGVDVKVVPDLLEFIALRARLEDLDGVPI
                     ININDVPLQGLNSALKRAIDIGCSAAAMAVLAVPFALIALAVRLTSPGPVFYRQERMG
                     LDGRAFTVYKFRSMFEDAERETGPIWAAEDDPRCTPVGRLLRRFDLDELPQIWNVLKG
                     DMSLVGPRPERPYFVEQFKQRYPQYMLRHKVRAGITGWAQVNGWRGNTSIEKRIEYDL
                     YYIGNWSLRLDFKIIWLTLVRGWIKPAY"
     gene            12496..13446
                     /locus_tag="PKK95_04945"
     CDS             12496..13446
                     /locus_tag="PKK95_04945"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC17592.1"
                     /translation="MAKPRAVITGAAGFIGSHLAETLLDLGYSVVGIDNLLTGDIGNI
                     AHLANRDFLFIKHDVTNYIYIDGPVDVVLHWASPASPIDYLELPIPTLKVGALGTHKA
                     LGLAKEKEARFVLASTSEVYGDPLEHPQRETYWGNVNPIGPRGVYDEAKRFAEAITTA
                     YHRYHGLDVKIVRIFNTYGPRMRVNDGRAVPTFISQALSNQDLTVFGDGRQTRSFTYI
                     SDLVDGVVRLMHSDENEPVNIGNPEEMTLLEIAQTIIRMTGSRSRIVHGPLPVDDPKV
                     RQPDITRARTLLGWEPKVPLEDGLARTIEYFRGKIRSVSC"
     gene            13457..13696
                     /locus_tag="PKK95_04950"
     CDS             13457..13696
                     /locus_tag="PKK95_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17593.1"
                     /translation="MPEVLKVLVPEVLKVLVPEVLKVLVRGCQHHQHGTSSTLGTFGT
                     APSAPPAPPAPPARQVQCYCYCCFFPGSLMIRAAW"
     gene            complement(13648..>14167)
                     /locus_tag="PKK95_04955"
     CDS             complement(13648..>14167)
                     /locus_tag="PKK95_04955"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOC17594.1"
                     /translation="IPAFVILQMYDQPGWALIAFAVAGITDALDGIVARLANQRTSLG
                     AWLDPMADKLLLVTTFVVLTLPGTHLVNRFPLWLTVLVISRDILIVLTVAVVSMVMGP
                     RTFRPSLWGKAATAIYLVSCVVLMWFNFLGRGSILVELAIWTSLAITLISGFHYIYHA
                     ARIINEPGKKQQ"
BASE COUNT         2027 a         5060 c         4912 g         2168 t
ORIGIN      
        1 cgctcggcgg cggtggccgc ctgctccagc aggtcggcca gcagcgcgcg gcgcgcctcg
       61 aggtcgtcct ggaggtgcga accctcgcgt gcgcgcatct cgttcagcgc cgcgagggct
      121 tcctcgaccg ccgcctcggc gagcgtgccg gcgtcaacgc cctcgacgtc ctccttcgcc
      181 gcctcgcgga tgaccagcgc ctgcggcagg cgcagcaggt cgcccggcgt cagcgcgccg
      241 tccaccagcc cctcttcgcg ggcgcgctcc aacgccccgg taaggcggcg aacgaacggc
      301 tcgttcaact cgacctcggg gggcggcgcc tgccgcagct gcaccgacac gctcagttcg
      361 acgcgcccgc gtgccacctg ccgctggacc gcggcacgca ggcgcccctc gatcggggcg
      421 aggcccggcg gaagcctcag ctgcaggtcc aggtagcggt ggttcaccgc ccggatggtc
      481 acacccaccg ccgcgctctc gtcctcgcgc gccgccgacg caaaaccggt catcgatcga
      541 atcatgtggt gctcacgatg gggccttgag ccttgagccc tgagccctga gcctgtccgt
      601 tcagccctcg ccccagggca ggccgagcgt ctcccggtgc agcgcgccgg cttccggccc
      661 gccgcgccgc ccggcggcca tctgcatggc atacagtctg gcatacgcgc cgcgaggccg
      721 cgccagcagt tcctcgtgcc gccccgtctc caccacccgc ccgcgatcga gcaccacgat
      781 caggtcggcc cgctgcacgg tcgagagccg gtgcgcgatg acgatcgccg tgcggttcgc
      841 catcaggttc gccagcgcct cctgcaccag ctgctccgac tcggagtcga gcgacgaggt
      901 cgcctcgtcc aggatcagga tcggcgagtt ccgcagcagc gcccgggcaa tcgcgatccg
      961 ctggcgctgg ccgcccgaga gcctctggcc gcgttcacca atcaccgtct ggtagccgcg
     1021 cggcatcgcc cggatgaact cgtgagcgta cgccgccctc gcggccgcct cgatctccgc
     1081 gtcggtcgcc ccggccgacc cgtaggcgat gttcctggcg atcgtgtcgt cgaagaggat
     1141 cgtctcctgg gtcaccatcc cgatctgccc gcgcagcgac gccagcgtca cgtcgcggat
     1201 gtcgaccccg tcgatgagga tggcgcccga ggtgacgtcg tggaaccgcg gcagcaggtt
     1261 caccagcgtc gtcttccccg cgccgctgcg ccccacgatc gccaccttct ccccggccgc
     1321 gaccgagagc gagacgccgc gcaggatcgg gtggcgccga tcgtcctcgt acccgaagcc
     1381 cacgtcccgg aactcgattc cgcgcgagag cggcgccagc gggcgggcgc ccggccggtc
     1441 gaggatctcg ttgtgggtgt cgagcagctc gaagatccgc tccgacgcgg cgatggtctg
     1501 ctgcagcgtc gcgttcaccc ggctcagctt ctttgccggc gcgtacatca gcatcagcgc
     1561 cgccatgaag gtggtgaact cccccaccgt catctcgcgg ctgccgtacc acagcgccgc
     1621 cacgatcccc agcgccccga gcagctccat caccggcggc aggctggaca gcaccgccgt
     1681 gaccttcatg ttcgcccggt acacccggtc ggccgccccc gcgaaccggc gctgctcctc
     1741 gcgctcggtg ccgaacgcct tgacgatccg gtgccccgtg aacgtctccg cgctcaggtg
     1801 cgacagccgc tccagctcct cctggctgcg cctcgtggtc cgcctcgccc gctgtcccag
     1861 ccgcaccagc gggtagacga ccagcggcgc gccggtcagg aagacgatcg ccagcctcgc
     1921 ctccaggtag aacagcagcg ccacgtaccc gacgagcgcc agcgactcgc gcaccaggtc
     1981 gcccagcgtc tcggacaccg cctgctggat catcgagacg tcgttggtca gccgcgacat
     2041 cagctgcccg gtggtccgtc gggcgaagaa ggacgccgac tgcccgatga tgtggccgta
     2101 gagcgcctgc cgcacgtcgc ggaccacgcg ctggccgacg tcggccatca ggtaggtcga
     2161 gaagtagctg cccagcccct tcagcaggta gaagcccacg atggccgagg acaccacccc
     2221 cacgttcgcc tgccgctcga gcacgtcgtc gaagacccac ttgatgagcg cggccaggca
     2281 ggccgaggcc gccgcgtaga ggaccatcca ggcgaaggcc gccaggaggc gtccgcggta
     2341 gggggcggcg aagcgcagga gtcgtcgaag ggcgttcaat ctgtgaaccg gtatcgcagg
     2401 agaacccaga gggccaccag cccgtcgcgc caggtgatct tcttgccctg ctcgtagccc
     2461 cgcccgtcgt aggtgatcgg gatctcgtag acccggtaac cccgcttgaa gatcttcgcg
     2521 gtcatctcgg gctcgatgcc gaacccgttc gacttcagat cgagaccccg cgcgatctcc
     2581 gcgcgcatca ccttgtagca ggtctccatg tcggtcagca tcgtgttgta cagcacgttg
     2641 gtcagcagcg tcacgatgcg gttgccgagg tagtgcgaga acatgaacac ccggtggcgc
     2701 ccgaggaacc gcgagccgta gaccacgtcg gcgcgccccg agcagatcag ctcgaccagc
     2761 tgcgggtact cctcgggcga gtactcgagg tcggcatcct ggatcaccac caggtcgccg
     2821 gtcacttccg agaagccgcg gcgcagcgcc gcgcccttgc cctggttgcg ctcttggagc
     2881 agcagctgga agcccagctc ggcctgcagc cggccgagga tctcgcgcgt cccgtcggtg
     2941 gacccgtcgt ccacgacgat cagctcggtc ctgagcggta cggcgtggac ccgccggatg
     3001 atctcctcga ccgtgtcacg ctcgttgtag acgggcatca cgaccgacag caggggatcg
     3061 accaggccac ccttcagccc cgccgcgggc gccgtcgcgg ccacgacctc cgtcttcatg
     3121 cgcggacctc tcggtacccg ttcgggtgtg cctcgagcca ggcccacgcc gtgcggacga
     3181 tcgtgtccag ttcctcgtag cgcggcttcc accccaattc ggccttgatg cgcgcgctcg
     3241 acgcgtagag tacggccggg tcgcccgggc gccgcggccc cagggcgtac gggacgggcc
     3301 gccccgtcac ccgctcgacc gccgcgatca cctcgcgaac cgagtacggc cggccgttgc
     3361 ccagattgta gacggtcgag gggcttcccg cctcgaggcc gtcgagagcg agcaggtgcg
     3421 cctgcgccag gtctgtgacg tgaacgtagt cgcgcaggca ggtgccgtcg ggcgtcgggt
     3481 agtcgctccc gaagacctga aggggcgtgc cccccgttgc cgcgcgcagc gcgagcggga
     3541 tcaggtggcg ctcggggtga tggtcctccc cgagcgtccc ggccgggtcg gcaccggcgg
     3601 cgttgaagta gcgaaggcag accgagcgga tgccgtaggc gcgttcgtag tgcgggagcg
     3661 cccgctcgac gacgagcttg gtctcgccat aggggttgat cgggcgcgcc gggtggtgct
     3721 cgtcgatcgg cacctgctcg ggctcgccga agacggcggc cgtcgaggag aagatgaaca
     3781 ccctcacctt ctcgcgggcc atcgcctcga gcacggcgag cgtcccgccg acgttgttgc
     3841 gatagtagcc agccggctcg gccaccgact cgcccaccgc cgtccaggcg gcgaagtgca
     3901 tcactgcggt gacgccgcgg gttgcgatcg cctcgcgcag cctcgagagg tcgcccgtgt
     3961 cgccctcgac gagatccacc ccgggcaccg ccgcacgatg gccctcgctc agatcgtcgt
     4021 agaccaccac gcggcggccc gcctcgcaaa gcgccttcac ggcgtggctg ccgatgtagc
     4081 ccgccccgcc ggtcaccagg acggcactgc ccctagcgcc cgacgccgta gtacgtgaag
     4141 ccatgggccc tcatccgctc cggtcgatag agattgcggc cgtcgaagac gaccggcgcc
     4201 cgcatgagct tccgcatctt ggacaggtcg ggctccctga actcgttcca ctcggtgacc
     4261 agcccgagcg cgtcggcccc cacgagggcg tcgtagctgc gcgcacagaa cttcacccgg
     4321 ctggcaagag ccgccttggc ggtgctcgag gctgccgggt cgtacacctg gacacgtgcc
     4381 cccgccgcga gcagccgctc gaccagcgcc agggccgggg cctcgcgcac gtccccggtc
     4441 ccgggcttga aggcaagccc ccagaccgcg atgacacgac cctcgagcga cccgaagtgg
     4501 cgctgcatcc ggcacgccag ccactctttc tgctcggcgt tcacccgctc ggccgcttcg
     4561 agcgcgcgga agccgtaccc gaggcctgcc gccagcgcca tcagggtccg cacgtccccc
     4621 gccaggttcg ccccgccgta gccggcgccg gcgaacaggc aggccgggcc gatgcggtgg
     4681 tcggcgccaa ccgcgcggcg cacctgctcc acgtcggcgc ccgcaagctc ggccacccgc
     4741 gccacctcgt tcatcaacga gatgcgcgcg gccagcagcg cgctggccgc cagcttcgac
     4801 agctcggcgc tggcacagtc catcacgagg accggcgcgc cggtgcgggt gaagggcgag
     4861 tagaggtcca ccagcagctg cgtggcccgc ggatcctcgg cgccgatcac cacgcggtcg
     4921 ggctcgagga agtcgcggac cgcggtgccc tgccggagga acgccgggct gctgaccacg
     4981 ctgaaggggt gcgtggtctc gcgccgcacg acctcgcggg ccttcgccgc cgtgcccacc
     5041 gggacagcgc tcgcgatggc cagcaccttg tagccgttca tcgcctgcgc cacctcgcgc
     5101 gcggcgtcga gaacgccttg cgcggcggcc tcgccgtcga cggaggatgc ggcctcgacg
     5161 gcgatgaaga cgatcgacga gtggcgggcc gccgtagcaa cctggctcgt gaaaaccagc
     5221 cgcttctcgg cgcggttccg ccgcacgatc tcctcgaggc ccggctcgac gaacgggatg
     5281 gccccgcgcc gcagcaggcg cagcctcgcc gggtcgcggt ccacggcgac cacgtcgttg
     5341 ccgttctccg cgaagcaggc ggcggcaacc agccccacgt acccggcccc gatgacggac
     5401 agcttcatgc gcgtcggctc ccggcgggag accactcaaa gagcccgaaa gtagcacaac
     5461 cgggataatc gagtcaagct actgatacgc aagcacttat aaggatcggc gggtttggtg
     5521 atcgagccgc gccggccctt ttcccgatcc tgaatcccga ttcctgctct tgccaccgcc
     5581 acctctgcca aggtaggttc ccaccgtgcg gctgctgctc gactatcgtc ccgccttgcg
     5641 ggaacgcacc ggggtcggcg agtacgtcca ccagttggcg cgggcactgg tccagctgtc
     5701 ggaggacggg gccgctgaag gcgggctgac ggtcgagctg ttcaccagct cgtggaagga
     5761 tcggccagat cctgccgctg ttgccgagct gggaccggtc cgggtcgtcg atcgccgcgt
     5821 gccggttcga acgctgaact gggcatggca ccgcctgcgg tggccgccgg ccgagctgct
     5881 cgcggggggg cgctacgatg tcgtccattc gccgcacccg ttgctgctgc cctcgcgccg
     5941 cgccgcgcgc gtgatcacga tccacgacct cgatttcctg ctgcaccccg agcggacgcg
     6001 ggaggagatc cgccgcgact accccgcctt cgtgcgtcgc gccgcgcata cggccgagct
     6061 ggtcatcgtt ccgtcggccc acgtggggcg ggtcgcggcc gggctgctgg agctggacgc
     6121 ctcggtggtg tgcgtgtgcc cgcagggagg gccggggtgg gagccggacc cggcgcaggc
     6181 caggggcaac cccgggggcc gctatatcct cttcctcggc acgctcgagc cccgaaagaa
     6241 cgtgcagggg ctgctcgatg cctacgcggc gctcgtcacg cggtggcccc aggcgccgcc
     6301 gctggtgctg gccggccacg gcacgcccga ggccgagcgc tggctcgagg ccacgctgcg
     6361 tccaccgctc gccgggcacg tcgagtgccg ggggtacgtg cccagccacg agcggcgggc
     6421 tctctacgag ggggcgcggt tactggtgct tccctcgttc gacgaggggt tcggcctgcc
     6481 cgtcatcgag gcgatgagtg ccggtatccc ggtcgtggcc tccgatcgcg gcgcgctgcc
     6541 cgaggtgtgc ggtggggccg ccttgctggt agaccccgac gatcgcggtt cgatttgcgc
     6601 gggcatcgag cgggtgctgc gcgaggcggg gctggccggg cggctggccg aacgcggcct
     6661 ggcgcgcgcg cgggatttca gctggcgccg tacggccgag ctgaccttcg aggcctaccg
     6721 cagggcgctg gtccggcgtg ccaggggagc cgcctgatgc gggtcgcgat cgacgggcgg
     6781 gaactgtgcg gacgcccgac gggcgtgggc cgcttcctgt cccacctgct cgcggcgtgg
     6841 gcaaagctgc ccgaggcgcg cgagcacgaa tacctcctct acgttccgga ggtccccctc
     6901 cgcctgccgc ctgatcttcg ggttcacacc cgcgtgctcc ccggcgcaag cggcacctgg
     6961 tgggagcagg tccggctggc ctcggccgtg cggcgcgatt gccccgacgt tctcttctgt
     7021 cccgcctaca cgtcgccgct cgccgcgggc gtgccggtgg tcgtcgcgct gcacgacgtg
     7081 tcgttcctgg cacaccccga gtggttcccg gcgggcagcg ggttgcggcg gcggctgctg
     7141 gccaggctgt ccgcgcggcg ggcggtgtcg gtcttcacga tctccgagtt ctcgcggggc
     7201 gagattgcct tccgcctgcg cgtcgcccgc gagcgggtgc gggtcgttcc gctggcgccg
     7261 ggagggccgc ccgcggctcc tggcctgttg cgcgagccgc tcgtgctcta cgtcgggtcg
     7321 atcttcaacc gccgccacct gcccgaactg atcgcggcgt gcgggaagct ggcgctcggg
     7381 caccccgagg tccgtctcga ggtggttggc gaggaccgga cctacccatc gcaggacctc
     7441 ggggcggcgg ccgcggcgag cgggatggag ggacgcctct cgctgcgctc gtacgtgccg
     7501 gacgaggagc tggccgtgct gtacggaaag gcctccgtct tcgcgttcct ctccgactac
     7561 gaagggttcg gcctgacgcc gctggaggcg atcgcggcgg gcgtgccggc ggtgcttgcc
     7621 gagaccgcgg tggcccgcga ggtttgcggc gacgcggcgc tctacgtgcc cacgggcgac
     7681 gtgggggcga tcgcggccgc cctggagtct gcgctcttcg atccggcaac gcggcggcgg
     7741 ctgcttgccg cgggcccggc caccctctcg cggtactcgt gggaccgcgt gggacgcgac
     7801 acgctggcgg tgctgctcga cgcggcccgg aggcgcgcct gagatgcagg ccgacctggc
     7861 gatcgtcatc gtcagctaca acgcgcggcg cgacctcgag cgcgcgctct cgtcgctcgc
     7921 ggttgccccg cctcggatcg cgcacgaaat cgtcgtggtg gacaacgcct cgtcggacgg
     7981 gagcaccgcg atggtgcgcg agcgctttcc cgaggtgcgg gtgatcgaag cgggcggcaa
     8041 catcgggttc gcccgggcga acaacctcgg aatccgcgcg accagcggcg acctgatcct
     8101 gctcctcaac agcgacacgg tcgtcccgcc cggggcggtg gacgccctgg tggccgacct
     8161 gctggcgcac cccgaggtgg cggtggcggg gccgcggctg gtggacggct cggggcgcgc
     8221 cgagtggtcg ttcggggaga tgatgacacc gtgggccgag ctgcgccgca agctggcggg
     8281 ccgactgtac gacgcgcgct tcccgctcgt gactcgcctg gtcgagcggc gcctggcggc
     8341 ggcccaggcg gtggactggg tgagcggggc ctgccttctc gtgcatcgcg ccgacgccga
     8401 cgcggtggga ctgctggacg agcggttctt cctctacacc gaggacgtgg acttctgcgc
     8461 cagcatccgg gcgcaggggc ggcaggtgcg gtacacgccg gcggcgcagg tcgtccacct
     8521 tcgggggcgg tcgcgctcgc acgatccgcg cggcgcgtcg gcccactatc gccggagcca
     8581 gctggccttc tacgccaagc accaccccgc ctggcaccgg gtcctgagag cctacctccg
     8641 gctgaagggc caactgccga aggacagctg acggctgaca gcggacagct gacagcgtta
     8701 ctccctcctg cacccgcctt cgccaagtgc tcgctacgcg agcggcttcg gcgggcaagc
     8761 cctctgcacc tcctgcacct cccgttatac tgtcagccgt gcggatcgcg atcgacgccc
     8821 gcaagtacct cgatttcggc atcggcacct acatcaggaa catcgtgcgc gagctggcac
     8881 gaatcgatgg ggagaccgag tacgtcctgc tgtgccgcga ggccgaccgc gaggcgatcg
     8941 ccacccttct gccgaagaac ttccgcccgg tcgtcgaacc ggcgcccccc tactcggttg
     9001 ccgagcaggt gcgggtgcca atcgcgctgc ggcgcgaacg ggtcgacctc ttccacgcgc
     9061 cgcactacgt gctgccgccg ctgatccact gccggtcggt ggtgacgatt cacgacatca
     9121 tccacctgct gtttccgcag tacctcccgg ggcggctggc gcacctctac gcacgggcgt
     9181 cgctgtgggg ggcggcgcgg cggtcggacc ggatcctgac ggtgtcggag acctcgaagc
     9241 tcgacatcct gcggcgcttc cgggtgccgg ccgacaagat cacggtcgtc tacaacgcga
     9301 ttgacgtccg gctcctcgcc gagccgagcc ctgaagagtt cgcgcgggtg cgggagcgct
     9361 atcagctcaa ccacccgttc gcgctgtacg tcgggaacat caagccgcac aagaacctcg
     9421 agcgcctgat cgaggcgttc gatcgcctgc ggcgcgagcc gggattcgag gcgctgcggc
     9481 tcctgatcat cggcgaccag atctcgaagt accagtcgct gcggcgcgcc gtccaccggc
     9541 acaagctgca caagcaggtg cggttcttcg ggttcgtgcc gctcgagacg ctggccgtgc
     9601 tctaccgtct cgccagcgtc ttcgtgttcc cgtcgctcta cgaggggttc ggcctgccgc
     9661 cggtcgaagc gatggcgtgc ggcctgccgg tcgtcacctc gaacatctcg tcgctgcccg
     9721 aggtggtggg cgatggggca atcctggtgg acccctacag ccccgactcg attgccgagg
     9781 ggatgcgggc cgcgctgacc gacgaggaac tgcgggcgac gctgcgcgag cggggcctgg
     9841 cgcgggcgcg ggagttctcg tgggagcagt cggtccggcg catccgcgag gtgtacgacg
     9901 aggtgatggc gcagccctat gagccgtgat cgccggcgcg gcttgccgcg catcgcgctc
     9961 gtccacgact ggctgaccgg gatgcgggga ggcgagaagg tcctcgaggc actctgcgag
    10021 ctgtaccccg aggcaccggt cttcacgatg gtgcacgtgc cggggaccga gtcgaggacg
    10081 atcgcacgcc accacccgcg cggtgcctgg ctgtctcggc tgcccggcgt ggggcgctac
    10141 taccggcacc tgctgcccct cttccccctt gccgccgaga cgttcgacct cgacgagttc
    10201 gatctcgtga tcagcagcag ccactgcgcg gtgaagtcga tcgtgcgccc gggccgggcc
    10261 cggcacctga gctactgcca cacgccaatg cggtacgtgt gggaccagcg cgacgcgtac
    10321 ttcgggcccg agcgactcgg cagggcggga tcgcgggccc tcaggccggt gctcgcctgg
    10381 ctggcccggt gggacgccgc gacggcgggg cgggttgacc gctatcttgc gaattcacag
    10441 cacgttgcgc gccggatcca ccgatactat aatcgccggg cggaggtggt cgcacccccg
    10501 gtcgacaccg ggttcttcca cccggacggg cgcttgccgg gcgacttcgc cctcgccgtc
    10561 tccgcgctgg ccccgtacaa gcgcctcgac gtcgcaatcg agggctgccg gctcgcgggg
    10621 attccactgc gaatcgttgg gtgggggccc gaagagcggc ggttgcgggc ccaggcccgg
    10681 ggcggcgcgg aactgctggg cacgctgagc gacgaggaga tccgcgagct ctaccgctcg
    10741 tgcgcgatgg tcctgctgcc cggcgaggag gacttcggta tcgtcccggt ggaggcgcag
    10801 gcctgcggcc ggcccgtcgt cgcggcggcg gccggcggcg cactcgagac ggtggtggac
    10861 gggacgaccg gcgtgctggt ggcgccgggg tcggccgagg cgctggcaga aggcatccgt
    10921 cgcgtccggg cgacccgctt cgacgaggcg gtgctgcgcc gacacgccga ggcgttcggc
    10981 cgcgagaggt tcaaggcccg gatttcggcg atcgttgacg agacgctggc gtcgggggcc
    11041 agcccgacca catggtgaag cgctacaacc gcctgctggc ggccgttcac gtcctctccg
    11101 acgccatcct ggcttccgcg gcgttcatgc tggcctattt gctccgcttc gaggcggggc
    11161 tcgtgccggt caccaagggc taccctcccc tttcccagta cctgcgaatt ctcccggtcg
    11221 tcgcgatcct ggtgcccttc gggtaccacc tgcagggtct ctaccggctg aggcgcggcc
    11281 gctcgcgggt ggacgatttc ttcgccgtct tcgtgggcac cgtcctcgca gtgatcttcg
    11341 gcatcgtcgc cacgctgtac ttccaggcct actacgccag ccccgccctg aaggaggtcg
    11401 gcgccttcca ggtgtcgcag atcgtctggg cggtgttcct ggtggcgaac atcgcgttga
    11461 ccttcgggtc gcgcgagatg atgcgccagc tgctcgagcg ccggtggcgg gccgggatcg
    11521 gcttgaagcg ggtgctgatc gccggcgcgg gcgacctggg gcgcgtggtg gtggatcgga
    11581 tcctcgacca ccgcgagctg ggcttccaga tcgtcgggtt cgtcgacgac cgcgccgggg
    11641 gcgatcacct ggggcaccgg gggctgccgc tgctcgggac gctccgcgag gtgtcggaga
    11701 tcgtgcagcg cgagcgggtg gatcacgtgt acgtgacgct gccgctcgag gagcacggga
    11761 agatcgtgca ggtgatcgag ggggcggcgc gcgagggggt ggacgtgaag gtcgtccccg
    11821 acctcctcga gttcatcgcg ctccgcgcgc ggctcgaaga cctggacggg gtcccgatca
    11881 tcaacatcaa cgacgtgccg ctgcaggggc tgaacagcgc cctcaagcgg gcgatcgaca
    11941 tcgggtgctc ggccgccgcc atggcggtgc tcgcggtccc gttcgcgctc atcgcgctgg
    12001 cggtgcggct gacgtcgccc gggcccgtct tctaccggca ggagcggatg gggctcgacg
    12061 gccgggcctt cactgtctac aagttccgct cgatgttcga ggacgccgaa cgcgagacgg
    12121 ggccgatctg ggcagcagag gacgaccccc gctgcacgcc cgtgggccgg ctgctgcgcc
    12181 ggttcgacct ggacgagctg ccgcagatct ggaacgtgct gaagggggac atgtcactgg
    12241 tcggtccccg ccccgagcgc ccgtacttcg tcgaacagtt caagcagcgc tacccgcagt
    12301 acatgctgcg gcacaaggtt cgcgccggca tcaccggctg ggcgcaggtg aacggctggc
    12361 gcggcaacac ctcgatcgag aagcgtatcg agtacgacct ctactacatc ggcaactggt
    12421 cgcttcgcct cgacttcaag atcatctggc tcaccctggt ccgcggctgg atcaagccgg
    12481 cgtactgaca ctcccatggc caagcctcgc gccgttatca cgggagcggc cggcttcatc
    12541 ggctcgcacc tggcggaaac cctcctcgac ctcggctaca gcgtggtcgg gatcgacaac
    12601 ctgctgaccg gtgacatcgg caacatcgcg cacctggcga accgcgattt cctgttcatc
    12661 aagcacgacg tcaccaacta catctacatc gacggcccgg tggacgtggt gctgcactgg
    12721 gccagcccgg ccagcccgat cgactacctg gaactgccga ttccgacgtt gaaggtcggc
    12781 gccctcggca cgcacaaggc gctcggcctg gcgaaggaga aggaagcgag gttcgtgctc
    12841 gcctcgacct ccgaggtcta cggcgatccg ctcgagcacc cgcagcgcga gacctactgg
    12901 ggcaacgtca acccgatcgg cccgcgcggc gtgtacgacg aggcgaagcg gttcgccgag
    12961 gcgatcacga ccgcttacca ccgctaccac ggcctcgacg tgaagatcgt ccggatcttc
    13021 aacacctacg ggccccgcat gcgggtcaac gacgggcgcg cggtgcccac cttcatctcg
    13081 caggcgctgt cgaaccagga cctcacggtg ttcggcgacg ggcgccagac ccggagcttc
    13141 acctacatca gcgacctcgt agacggagtg gtccgcctga tgcactcgga cgagaacgag
    13201 ccggtgaaca tcgggaaccc ggaggagatg accctgctcg agatcgccca gacgatcatc
    13261 cgcatgacag gctctcgaag ccgcatcgtc cacggtcccc tcccggtgga cgaccccaag
    13321 gtccgccagc cggacatcac ccgcgcccgc acgctgctcg gctgggagcc gaaggtcccg
    13381 ctcgaggacg ggctcgcgcg gacgatcgaa tacttcagag gaaagatccg aagcgtttcg
    13441 tgctgacggt gcaggggtgc cggaggtgct aaaggtgctg gtgccggagg tgctaaaggt
    13501 gctggtgccg gaggtgctga aggtactggt cagagggtgc cagcaccacc agcacggcac
    13561 ctccagcacc ttgggcacct tcggcactgc accttctgca cccccggcac ctcctgcacc
    13621 ccctgcacga caggtgcagt gctactgcta ctgctgcttc ttccccggct cgttgatgat
    13681 ccgcgcggcg tggtagatgt agtggaatcc cgagatcagc gtgatggcga gcgaggtcca
    13741 gatcgccagc tccaccagga tcgagcctcg cccgaggaag ttgaaccaca tcaggacgac
    13801 gcagctcacc aggtagatgg ccgtggccgc cttgccccag agcgagggcc ggaaggtgcg
    13861 gggccccatc accatgctca ccacggccac cgtcaggacg atcaggatgt cgcggctgat
    13921 caccagcacc gtgagccaca gcggaaagcg gttcaccagg tgcgtgccgg gcagggtcaa
    13981 taccacgaag gtggtcacca gcagcagctt gtcagccatt gggtcgagcc aggccccgag
    14041 gctcgtccgc tggttggcga ggcgcgccac gatgccgtcc agcgcgtcgg tgatgccggc
    14101 caccgcgaaa gcgatcagcg cccatcccgg ctgatcgtac atctgcagga tcacgaatgc
    14161 cgggatc
//
LOCUS       DAOPQX010000058        14139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5049_length_14139_cov_4.522508, whole genome shotgun sequence.
ACCESSION   DAOPQX010000058 DAOPQX010000000
VERSION     DAOPQX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5049_length_14139_cov_4.522508"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="PKK95_04960"
     CDS             complement(<1..1897)
                     /locus_tag="PKK95_04960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC17595.1"
                     /translation="MPRARDGGGREEPVRGGLLSPGVSVERSGRERALEAALHQLVPS
                     WHQDYPDRPDAHVTAVRRMPAAPARTAPYPEALDPRLCETLRARGISELYTHQAAAVA
                     HILAGRHVVVTTPTASGKTLCYNLPVLHRILVEPSTRAMYLFPTKALAQDQLAELDRL
                     SQALNESAGVELGVFTYDGDTPQDARRAIRARAQVVLTNPDMLHAGILPHHPRWARLF
                     ENLRYVVIDELHAYRGVFGSHLANVLRRLHRVCRHYGSEPQFVCSSATIANPRELAER
                     LTERPFELVEESGAPRGEKYFFFVNPPIVNRQLGIRRSYTQETRQVALEFLRRGLQVI
                     VFAQSRLVTEILTKYLKESFQGPPGADEVVRGYRGGYLPNRRREIEKGLREGEIRAVV
                     ATSALELGIDIGALDVAVLAGYPGTIAATWQRAGRAGRRSGTSAAVLVASSAPLDQYV
                     ARTPSYFFDASPEHALINPDNLQILLAHIKCAAFELPFGVNEEFGRQPVQEILQILSE
                     EGLVQRAAAQAPGEEEHWHWTSESYPADAISLRSISSDNFVVVDNTDHTRVIAETDFT
                     SAPSTLHEKAIYLLEGRLFQVERLDYDNRKAYVRAVDCDYYTDAIRYDRVTILDTFDS
                     SALHAHGE"
     gene            complement(2073..3173)
                     /gene="galK"
                     /locus_tag="PKK95_04965"
     CDS             complement(2073..3173)
                     /gene="galK"
                     /locus_tag="PKK95_04965"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOC17596.1"
                     /translation="MTIDPSRFEALFGRPPELEASAPGRVNLIGEHTDYNEGFVLPTV
                     IPQRTHVALGARSDRTVRAFSAQVDGQAPFEYRLGSEQPRRDWTDYVQGITWVLGEEH
                     FAFEGADLFIESSVPVGSGLSSSAALLVAVLRAFREEYSLPLDDVALARLAKRAENDF
                     VGAPVGIMDQMVCSLASDRQALFLDARTLEYRLLALPPEIELVVINSGVAHQHSAGDY
                     RTRRAECERAAAALGVPALRDLTPADLPRAMALPEPLGRRARHVVTEDERVLQAVAAI
                     ERGDLAELGRLFYASHASMRDDYEVSVRETDLLVELARAESAVHGARLTGGGFGGSVV
                     ILAEPGKSGGTAARVKAEYDRQSGRVATVLVP"
     gene            complement(3170..4240)
                     /gene="galT"
                     /locus_tag="PKK95_04970"
     CDS             complement(3170..4240)
                     /gene="galT"
                     /locus_tag="PKK95_04970"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOC17597.1"
                     /translation="MKPDGRQLILYGHRPIPENLEAPSPPGAGHRPNSHLRWHPLRGE
                     WIAYASHRQNRTFLPPPEYNPLAPTADPASPTEVPEGEWDVAVFENLFPTLLATSHDP
                     PQIEVPTRPGRGACEVVVFTQDPTASLGRLPLDHIELLAEVWADRYRELGAREDVEYV
                     FEFENRGVEVGVTLGHPHGQIYAYPFVPPIPAHELANQAAYLAEHGTGLLEDVVARET
                     ADGRRVVYGGSLVAAFIPAFARYSYEVWIAPRRPAGSIADLTSDERRDFARALKTVLM
                     KYDGLWSRPFPYVMAFHQAPTDGRVHPEAHFHVEFYPAYRMPGRLKYLAGSELGAGVF
                     TADTLPEEKAAELRAVEVQEYR"
     gene            complement(4320..5099)
                     /locus_tag="PKK95_04975"
     CDS             complement(4320..5099)
                     /locus_tag="PKK95_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC17598.1"
                     /translation="MDLQLTGKVALITGSSRGLGLASARALASEGCKVCLCARTPGPL
                     DEAAQEVSVLAPSPDAVLAVEADLTTALGVEKVIDAAVRRFGGLDILVNNLGLAKGGD
                     IVETSDAEWQEAFDSTLYPAIRASRLAVPQMKARGGGVIIMIASIYGREAGGRMTYNA
                     VKAAEISLGKAMARQLAPFHIRVNSVCPGSILFPGGSWWKRVQADPERMAEFVRRELP
                     FGRFGRAEEVGDVVAFLASPRASWVSGAAVVVDGCQGWSNI"
     gene            complement(5152..5955)
                     /locus_tag="PKK95_04980"
     CDS             complement(5152..5955)
                     /locus_tag="PKK95_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC17599.1"
                     /translation="MRRHFERAGRLVSYLEVEPAAAAGRGTLVLVHAFPLAAEMWEPQ
                     LAAPPEGWRVVAPDLAGFGRSFPAPASATFEDDAGDLLALLDHLGEKQAVVAGLSMGG
                     YVAFALLRLAPERVRGLVLADTRPDADSPEARRSRQDMLNTLDRDGVEAVVEGMLPRL
                     LGATTLETRPGVAARVREIGRRQRTEGIRAAIHRLMGRPDSTPLLPAIRCPALVIAGA
                     QDAITGPDIARGMQREIPGARLALLESAGHLSNLEQPDAFNAALGEFLS"
     gene            complement(6039..8804)
                     /locus_tag="PKK95_04985"
     CDS             complement(6039..8804)
                     /locus_tag="PKK95_04985"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOC17600.1"
                     /translation="MQRGVTQETGSAAQKVVEHDAPGLTYSRSFALSGRDPFADIAWE
                     LRSALITNDKGDVVFEQKGVEVPAFWSQQATNIVVSKYFRGAVGTPERETSVRQLIGR
                     VTDAIVAWGRAGRYFASEDHLQAFGDDLRHLLVHQMASFNSPVWFNCGIEKQPQCSAC
                     FINSVEDTLDSILTLAKTEGMLFKFGSGTGTNFSSLRSSKESLAGGGTASGPVSFMKG
                     YDAFAGVIKSGGKTRRAAKMVILNADHPDIVEFINCKVDEERKAWALIDAGYDGSFAG
                     PAYSSVFFQNSNNSVRVSDDFMRAVLDDGTWETRAVTDGRVMGTYKARELMRMIAEAT
                     HVCGDPGMQFDTTVNEWHTCPASGRINASNPCSEYMFLNDSACNLASLNLMKFVDEAG
                     EFDVQAFRAAVDTLITAQEIIVDNASYPTRAIERNSKEYRPLGLGYANLGALLMARGL
                     PYDSDAGRHYAAAITALMTGEAYAQSARISRDHGGPFSAFPKNREPFLRVMRKHRDAL
                     KDVNRGLVPPPLYRGAQRAWDEAVELGEESGYRNAQTTVLAPTGTIGFMMDCDTTGIE
                     PDIALVKYKKLVDGGLMKIVNQTIPLALARLGYTPEQVKAIVAHVDEMETIEGAPFLK
                     EKDLPVFDCAFKPARGTRSIHYLGHIRMMGAAQPFLSGAISKTVNVPKEATADEIMQA
                     YIEAWRLGVKAISIYRDGSKRTQPLNTSRDAAPGAAARAEAQAVPRPVRRKLPDEREA
                     ITHKFEIAGHEGYITVGLFEDRTPGEIFLVMAKEGSTISGFADAFAQAVSYALQYGVP
                     LQVLVDKFSHVRFEPAGITRNPQVRIAKSIVDYVFRWLATKFLSPEAQFYAGVNQSEA
                     GPADEGPRQLTLPPLGLEADRSPRNPGLAIQNQEDAPPCSTCGAIMVRSGSCYKCINC
                     GSTSGCS"
     gene            complement(9119..9670)
                     /locus_tag="PKK95_04990"
     CDS             complement(9119..9670)
                     /locus_tag="PKK95_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17601.1"
                     /translation="MRGWTGTALVALLLVAGCAKSRPQLQPDATPLLVPPAPPRVIVP
                     PEPEPIPLEPLPEPSELTSRPTRRSVPRPESRTAKPEAPPPSAEAPAVPPVASPAESL
                     EPVMSTSPGEVERRVRLQLQRASSDLNRVDYGALSQDGQGQYDMAKRFIEQADEALRD
                     RNLVFAGKLAEKAAGIASVLLGR"
     gene            complement(9756..11687)
                     /locus_tag="PKK95_04995"
     CDS             complement(9756..11687)
                     /locus_tag="PKK95_04995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC17602.1"
                     /translation="MHLSIKAKQIGGISLIVGLAVVALSAIHLSTLVKISLEESGARA
                     DLLVAAIFQRARLVVPGQADPYEALRQDGGLRSILESSAYSKSVVYAAIVDPDGVAVV
                     HSVPDRQGKPLAPAGGLDEVLALPRRQQVARVYSSESLTLEVGQPLRLGTRDFGAIRV
                     GVSTLLIRQELWEALKPAMVTALIALALAILVAGVFTRLMLRPIHVIRGGLTRLGRGE
                     FGVKLDLPQQDEFGELGNFFNAVSAQLSADRSQLAGQKANLEAAVEHIEDEVALINPA
                     GELLFANPAMRRALPPESFGRPIDDVLPAGHPYRELVQATLREGQSRGPVSASIPGPL
                     PPGAAAPEGDEAAPPATERLVMAHAIKDLDQQLVGVMLVSRDVEYMGRVQSMLNYSRK
                     LVALGRLSAGVAHEVKNPLNAMTIHLELLKQKLSPTTVRRRPRAAVAVPAPASGLGLS
                     TPDPVEVNGPDLSAALDHVKIIGSEIQRLDQVVQGFLKFTRAEDLKLQDLSLGSVVDE
                     VVRIVEPECAKSGVALTVTGFDSLPPVSGDSTMLRQAFLNLALNACQAMPNGGQLRIA
                     ASPLPGRRVEVRFEDTGIGIKPEHLERIFDLYFTTRDRGSGIGLSMVYRIVQMHDGEI
                     EVESTEGRGTTFRIVLRQA"
     gene            complement(11692..13101)
                     /locus_tag="PKK95_05000"
     CDS             complement(11692..13101)
                     /locus_tag="PKK95_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC17603.1"
                     /translation="MEHVHPAAADAESGEAPGVTERVLIVEDDPSTRLGLTELVRNWG
                     YLADSAEDGEEGLQKITSFRPAIVVTDLVMPRMDGHQLLKALREQDPDIAVVILTAQG
                     SVESAVEAIKDGAYDYLTKPVDPQRLQILLANVVERQETLREVKALRRQLRDQGAFGR
                     MIGASPGMRKVYRVIEQAAPTSASVLISGESGTGKELVAQTIHQLSPRANAPFVAINC
                     AAIPETLLESEIFGHEKGAFTGAADRRQGCFELADRGTIFLDEIAEMTPATQVKLLRV
                     LQERSFRRLGGRQEQKVDVRVIAATNADPDEAVKKGKLREDLYYRLNVFGITLPPLRD
                     RKDDLPLLTQFFLSEFNQRNGKSIAALNNDAMRILDGYGWPGNVRELRNVIERATILT
                     DGQFIEPRHLPAALVQAGETARPSLQLPPGTTVEEAERRLIQMTLEHTRDNKTRAAEI
                     LGISLKTLHNKLNKLKLRK"
     gene            13336..14037
                     /locus_tag="PKK95_05005"
     CDS             13336..14037
                     /locus_tag="PKK95_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOC17604.1"
                     /translation="MDDDSLAQGLPPPLRDPSGGLNARAAAVIEVILCSGLPTQLALA
                     GLLALAGIAPYGPDGALSPAYVFVLPLADAVLLLGLVTWFLRLHGERVSDVMLGGRPL
                     LREAAVRGSLGVEGRDIGTVVFPDAVLKVHLHAALPVRAERRQADLRRQGIDMPAGEV
                     SRDLEERDRRDSTRADSPLRQAEGALVLDTSRCSIDEQVDAILRAYFAAVRATRAPEG
                     PAGPEASRPPEGERP"
BASE COUNT         2019 a         4912 c         5028 g         2180 t
ORIGIN      
        1 cctcgccgtg agcgtggagc gccgagctgt cgaaggtgtc gaggatggtg acccggtcgt
       61 agcggatggc gtcggtgtag tagtcgcagt ccaccgcccg cacgtatgcc ttgcggttgt
      121 cgtagtcgag gcgctcgacc tggaagagcc gaccctcgag caggtagatc gccttctcgt
      181 ggagcgtgga cggcgcgctg gtgaagtcgg tctcggcgat caccctggtg tgatcggtgt
      241 tgtccaccac cacgaagttg tcggacgaga tcgaccgcag gctgatcgcg tcggccgggt
      301 acgactcgct cgtccagtgc cagtgctcct cctcgccggg cgcctgcgcc gcggcgcgct
      361 gcacgagccc ctcctcggag aggatctgga ggatctcctg caccggctgg cgcccgaact
      421 cctcgttcac gccgaacggc agctcgaagg cggcgcactt gatgtgcgcg agcaggatct
      481 gcaggttgtc ggggttgatc agcgcgtgct cgggcgacgc gtcgaagaag tacgatggcg
      541 tccgtgcaac gtactggtcg agcggggcgc tgctggctac cagcaccgcg gccgaggtgc
      601 ccgaccgccg gcccgcacgc ccggcgcgct gccaggtggc ggcaatcgtc cccgggtagc
      661 cggccagcac ggccacgtcg agcgcgccga tgtcgatccc cagctcgagc gcgctggtcg
      721 cgaccaccgc ccggatttcc ccctcgcgca accccttctc gatctcgcgg cgacggttgg
      781 gcaggtaccc gccccggtag ccgcgcacga cctcgtcggc gccgggaggc ccctggaacg
      841 actccttcag gtacttggtc aggatctccg tcaccaggcg gctctgggcg aacacgatca
      901 cctgcaggcc ccggcgcagg aactcgagcg ccacctgccg ggtctcctgc gtgtacgagc
      961 gccggatgcc gagctgcctg ttgacgatcg gcgggttgac gaagaagaag tacttctcgc
     1021 cgcgcggcgc cccgctctcc tccaccaact cgaacggacg ctcggtgagc cgctcggcca
     1081 gctcacgcgg attggcaatg gtcgccgacg agcagacgaa ctgcggctcg gagccgtagt
     1141 gacggcagac ccggtgcagc cggcgcagca cgttcgcgag gtggctgccg aacaccccgc
     1201 ggtaggcgtg cagctcgtcg atcaccacgt agcgcaggtt ctcgaagagg cgggcccagc
     1261 gcgggtggtg cggcaggatg cccgcgtgca gcatgtccgg gttggtgagc acgacttgcg
     1321 cccgcgcacg gatggcccgc cgcgcgtcct ggggtgtgtc gccgtcgtag gtgaacaccc
     1381 ccagctcgac cccggccgac tcgttgagcg cctgcgacag ccggtccagc tcggccagct
     1441 ggtcctgcgc gagcgccttg gtcgggaaga gatacatcgc ccgcgtcgaa ggctcgacca
     1501 ggatccggtg caggaccggc aggttgtagc agagtgtctt gccagaggcg gtcggggtgg
     1561 tcacgaccac gtggcgcccg gcaaggatgt gcgccaccgc cgcggcctga tgcgtgtaga
     1621 gctcgctgat gccgcgggcc cggagggtct cacacagccg gggatccagg gcctcggggt
     1681 acggcgccgt gcgggcggga gcggccggca tcctccgcac cgccgtgacg tgggcgtcgg
     1741 gccgatcggg gtagtcctgg tgccacgagg gcacgagctg gtgcagggcc gcctcgagcg
     1801 cacgctcgcg gcccgagcgc tccacgctga cgccgggtga cagcagcccg cctcgcaccg
     1861 gctcctcccg gccgccccca tcacgtgctc tcggcatggt cggcgatcgt actagccaag
     1921 gtggtaccga tcaagaggcg aaaggcgaaa aaagggagaa attgctgagc tcggctcgcg
     1981 cgcgagccgt gcgactaagt gtgcagccgt gagccgtgag ccctgagccc tccgtgagcc
     2041 ttgagccctg agccgtgagc cttgtgcctt tcttatggca ccagcaccgt ggcgacccgt
     2101 cccgactgcc ggtcgtactc cgccttcacg cgcgccgcgg tacccccgct cttccctggc
     2161 tcggcaagga tgacgaccga gccgccgaac ccgccgcccg tcagccgggc gccgtggacc
     2221 gcgctttccg cccgcgccag ctcgaccagc aggtcggtct cgcgcaccga cacctcgtaa
     2281 tcgtcgcgca tcgaggcatg cgacgcgtag aacagccggc ccagctcggc caggtcgccc
     2341 cgctcgatcg cagcgaccgc ctggagcacc cgctcgtcct cggtcaccac gtgccgcgcg
     2401 cgccggccca gcggctcggg cagcgccatc gcccggggca ggtccgcggg cgtcaggtcg
     2461 cgaagcgccg gcacgccgag cgccgccgcg gcccgctcgc actccgcccg tcgcgtgcgg
     2521 tagtcgccgg ctgaatgctg gtgggccaca ccgctgttga tcacgaccag ctcgatctcg
     2581 ggcggaagcg ccaggaggcg gtactcgagc gtgcgggcgt cgaggaagag cgcctggcga
     2641 tcggaggcga ggctgcacac catctggtcc atgatcccga cgggcgcgcc gacgaaatcg
     2701 ttctctgccc gcttggcgag ccgcgccagt gccacgtcgt cgagcgggag cgagtactcc
     2761 tcgcgaaacg cccgcaggac ggccaccagc agggccgcgc tcgacgagag accgctcccc
     2821 acgggcaccg acgactcgat gaagaggtcg gccccctcga aggcgaagtg ctcttccccc
     2881 agcacccagg tgatgccctg gacgtagtcg gtccagtcgc ggcgcggctg ctccgagccg
     2941 aggcggtact cgaaaggcgc ctgcccgtcc acctgtgcgc tgaaggcccg cacggtgcgg
     3001 tcggatcgcg cgcccagcgc cacgtgggtc cgctgcggga tcaccgtggg caggacgaac
     3061 ccctcgttgt agtcggtgtg ctcgccaatg aggttgacgc gcccgggggc cgacgcctcc
     3121 agctcgggag gccgtccgaa cagcgcttcg aatcgggacg ggtcgatcgt catcggtact
     3181 cctggacttc caccgccctc agctccgccg ctttctcctc gggcagcgtg tcggccgtga
     3241 acaccccggc gcccagctcg ctgccggcaa ggtacttcaa acggccgggc atgcggtagg
     3301 ccgggtagaa ctcgacgtgg aaatgcgcct cggggtggac gcggccgtcg gtgggggcct
     3361 ggtggaacgc catcacatag gggaacgggc gcgaccagag cccatcgtac ttcatcagca
     3421 cggtcttcag cgcccgggcg aagtcacggc gctcgtcgga ggtgagatcg gcgatcgagc
     3481 cggccgggcg cctcggggca atccacacct cgtacgagta gcgggcgaag gccgggatga
     3541 aagcggccac caggcttccc ccgtagacga cccgccgccc gtcggcggtc tcgcgggcga
     3601 cgacgtcttc gagcaggccc gtgccgtgct cggcgaggta cgccgcctgg ttcgccagct
     3661 cgtgcgcggg aatcggcggc acgaacgggt aggcgtagat ctggccgtgg gggtggccga
     3721 gggtcacgcc gacttcgacg ccgcggttct cgaactcgaa gacgtactcg acgtcctcgc
     3781 gcgctcccag ctcgcggtaa cggtcggccc acacctccgc gagcagctcg atgtggtcga
     3841 gcggcagccg gccgagcgag gcggtcgggt cctgcgtgaa gaccaccacc tcgcacgcgc
     3901 cgcgcccggg acgcgtcggc acctcgatct gaggcgggtc gtgggacgtc gcgagcagcg
     3961 tcgggaacag gttctcgaag acggccacgt cccactcgcc ctccggtacc tcggtggggc
     4021 tggcggggtc ggcggtcgga gcgagcgggt tgtactcggg cggggggagg aaggtcctgt
     4081 tctggcggtg gctggcgtat gcgatccact cgccgcgcag cgggtgccac cgcaggtggc
     4141 tgttcggccg gtggccggcg ccgggcggcg aaggggcctc caggttttcg ggaatgggac
     4201 gatggccgta caggatcagc tgccggccgt caggtttcac gaaacgccgt ttgtgcatat
     4261 gaccagccgc ccaccccctg cattcttcct cttacacagg cgctctactc agcggcctgc
     4321 tagatgttcg accacccctg gcagccgtcc accaccacgg cggcgccgct cacccagctt
     4381 gctcgcggcg acgccaggaa cgcgacgacg tcgcccacct cctcggcgcg cccgaagcgg
     4441 ccgaagggca gctcgcgccg cacgaactcc gccatcctct cgggatcggc ctggacgcgc
     4501 ttccaccagg atccgccagg gaagaggatc gaccccgggc agacgctgtt gacgcggatg
     4561 tggaagggcg cgagctggcg ggccatcgcc ttgcccaggc tgatctccgc cgccttgacg
     4621 gcgttgtagg tcatgcgtcc gcccgcctcc cgtccgtaga tcgacgcgat catgatgatg
     4681 acgccgccgc cccgcgcctt catctggggg acggccagcc ggctggcgcg gatggccggg
     4741 tagagcgtcg agtcgaacgc ctcctgccat tcggcgtcgg aggtctcgac gatgtcgccg
     4801 cccttggcga ggccaaggtt gttgaccagg atgtcgaggc cgccgaagcg ccggacggcg
     4861 gcgtcgatca ccttctcgac gccgagggcg gtggtcaggt cggcctccac ggcaagcacg
     4921 gcgtcgggcg acggggccag gaccgacacc tcttgcgcgg cttcgtccag gggacccggc
     4981 gtgcgcgcgc aaaggcacac cttgcaccct tcggaggcca gggcgcgggc gctggcgagg
     5041 ccgagaccgc ggctcgagcc ggtgatcagg gcgaccttgc cggtcagctg cagatccata
     5101 gccttgctag cataaagcga acacgtcagt ttgcgcgcgc gtcagttcgc gtcagctgag
     5161 gaactcgccg agcgcggcgt tgaacgcgtc gggctgctcg aggttcgaca ggtgcccggc
     5221 cgattcgagc agcgccaggc gcgcgcccgg gatctcgcgc tgcatcccgc gcgcgatgtc
     5281 gggacccgtg atggcgtcct gggcaccggc tatgacgagc gccgggcagc ggatggcggg
     5341 aagcaggggc gtcgaatcgg gccggcccat cagccggtgg atggccgcgc ggatgccttc
     5401 ggtgcgctgg cgccggccga tctcgcgcac tcgggccgcc acaccggggc gggtctcgag
     5461 cgtcgtcgcg ccgaggagcc gcggcagcat cccctcgacg acggcttcga cgccgtcgcg
     5521 atcgagcgtg ttcagcatgt cctgcctcga ccggcgcgcc tcgggggagt cggcgtccgg
     5581 ccgcgtgtcg gcgagaacga gcccgcgaac ccgctcgggc gccagccgta ggagtgcgaa
     5641 ggcgacatac ccgcccatcg acaggccggc aacgaccgcc tgcttctcgc cgaggtggtc
     5701 aaggagcgcg aggaggtcac cggcgtcgtc ctcgaaggtg gccgaggcgg gcgcggggaa
     5761 cgagcggccg aagccagcca aatcgggtgc gacgacgcgc cacccttcgg ggggcgcggc
     5821 cagctgcggc tcccacatct cggccgcgag cgggaaggcg tgcacgagga cgagggtgcc
     5881 gcggccggcg gcggccgcag gttcgacctc gaggtagctg acgaggcgtc ccgcgcgttc
     5941 gaagtgtctt cgcatccccg actcccctgc aacaggtgga ccgcgtacaa gcggaggggc
     6001 ggcccctgcc gccccaacag tcccccgcgg ctgctgcgtc agctgcagcc gctcgtgctc
     6061 ccgcagttga tgcacttgta gcagctgccg ctgcggacca tgatggctcc gcaggtcgag
     6121 caggggggcg cgtcctcctg gttctggatg gcgaggcccg ggttcctcgg tgaccggtcg
     6181 gcctcgaggc cgagcggcgg cagggtgagc tgccgcggcc cctcgtcggc cggcccggcc
     6241 tcgctctggt tcacgcccgc gtagaactgc gcctcgggcg agaggaactt ggtcgccagc
     6301 cagcggaaca cgtagtcgac gatcgacttc gcgatccgca cctgcgggtt gcgggtgatg
     6361 ccggccggct cgaagcgcac gtggctgaac ttgtccacca gcacctgcag cggcacgccg
     6421 tactgcagcg cgtaggagac ggcctgcgcg aacgcgtcgg cgaagcccga gatggtggac
     6481 ccctccttgg ccatcacgag gaagatctcg cccggcgtcc ggtcctcgaa cagcccgacg
     6541 gtgatgtacc cctcgtggcc ggcgatctcg aacttgtggg tgatcgcctc gcgctcgtcg
     6601 ggcagcttcc ggcggacggg gcggggcacc gcctgggcct cggcgcgggc ggcggcgccc
     6661 ggggccgcgt cgcgcgaggt gttcagcggc tgggtgcgct tgctgccgtc gcggtagatc
     6721 gagatggcct tgacccccag ccgccaggcc tcgatgtagg cctgcatgat ctcgtcggcg
     6781 gtcgcctcct tcggcacgtt gaccgtcttc gagatcgcgc cggagaggaa gggctgggcg
     6841 gcgcccatca tccggatgtg gccgaggtag tggatcgagc gggtgccgcg cgccggcttg
     6901 aacgcgcagt cgaagaccgg gaggtccttc tccttgagga acggcgcccc ctcgatcgtc
     6961 tccatctcgt ccacgtgggc gacgatcgcc ttcacctgct cgggggtgta gccgaggcgg
     7021 gcgagcgcga gcgggatcgt ctggttgacg atcttcatca ggccgccgtc caccagcttc
     7081 ttgtacttga ccagggcgat gtcgggctcg atcccggtgg tgtcgcagtc catcatgaag
     7141 ccgatggtcc cggtcggcgc gagtaccgtg gtctgcgcgt tccggtaccc cgactcctcg
     7201 cccagctcga cggcctcgtc ccacgcccgc tgggcgccgc ggtagagggg cggcggcacg
     7261 aggccgcggt tgacgtcctt gagggcgtcg cggtgcttcc gcatcacgcg gaggaacggc
     7321 tcgcggttct tggggaaggc ggagaacggc ccgccgtggt cccgcgagat ccgggccgac
     7381 tgggcgtagg cctcgccggt catcagcgcg gtgatcgcgg ccgcgtagtg acgcccggcg
     7441 tcgctgtcgt agggcagccc gcgcgccatc agcagcgccc cgaggttcgc gtagccgagg
     7501 ccgagcggcc ggtactcctt gctgttgcgc tcgatcgccc gggtcgggta gctggcgttg
     7561 tcgacgatga tctcctgggc ggtgatgagc gtgtcaaccg ccgcgcggaa cgcctgcacg
     7621 tcgaactcgc ccgcctcgtc cacgaacttc atcaggttga gcgaggcgag gttgcacgcc
     7681 gagtcgttga ggaacatgta ctcggagcac gggttcgacg cgttgatccg gcccgatgcc
     7741 gggcaggtgt gccactcgtt gacagtggtg tcgaactgca tgcccgggtc gccgcacacg
     7801 tgggtcgcct cggcgatcat ccgcatcagc tcgcgagcct tgtaggtgcc catcacccgt
     7861 ccgtcggtga ccgcccgcgt ctcccaggtc ccgtcgtcga gcacggcccg catgaagtcg
     7921 tccgacaccc ggacactgtt gttcgagttc tggaagaaga ccgaggagta cgccgggccg
     7981 gcgaacgagc cgtcgtagcc ggcgtcgatc agggcccagg ccttcctctc ctcgtccacc
     8041 ttgcagttga tgaactcgac gatgtcgggg tggtcggcgt tgaggatcac catcttggcg
     8101 gcgcgccgcg tcttcccgcc cgacttgatc acgccggcga aggcgtcgta gcccttcatg
     8161 aacgagacgg ggcccgaggc cgtgccgccg cccgccagcg actccttcga cgagcggagc
     8221 gacgagaagt tggtcccggt gcccgacccg aacttgaaga gcatcccctc ggtcttggcc
     8281 agcgtcagga tcgagtcgag cgtgtcctcg accgagttga tgaagcaggc cgagcactgc
     8341 ggctgcttct cgatgccgca gttgaaccag accgggctgt tgaaggacgc catctggtgg
     8401 acgagcaggt ggcgcaggtc gtcgccgaag gcctgcaggt ggtcttcgga ggcgaagtag
     8461 cggcccgccc gcccccacgc gacgatcgcg tcggtcaccc gcccgatcag ctgccgtacg
     8521 ctcgtctcgc gctcgggcgt cccgaccgct ccgcggaagt acttcgagac gacgatgttg
     8581 gtggcctgct gcgaccagaa ggcgggcacc tcgaccccct tctgctcgaa gaccacgtcg
     8641 cccttgtcgt tggtgatgag cgccgagcgt agttcccaag cgatgtccgc gaaggggtcg
     8701 cggcccgaca acgcgaagga tcgcgagtag gtgaggccag gtgcgtcgtg ctcgacaacc
     8761 ttctgagcgg ccgatcccgt ctcctgggtc acgcctctct gcataccttc cctccgacgc
     8821 gcccccgggc ccccgggagg cgtgccactc gcttgcccat ttggtcggcg acgggcgctg
     8881 ggaacgctcc accgccacgc gtccgcccgg gttcgaccgc cggagaagca cctgggcagg
     8941 acagccacta tggacgaccg cagccgtcct tgtcaagatc ggagctacaa catattgtgc
     9001 ctgtctttta ggtcagtcgc aatacgtggg gtttctggcc gaaaacggac gggtgtccgc
     9061 aaggcggaat tggagtatca gcctgcagtg gtcagctgtc gattggcttg ggtcttcgct
     9121 accgtccgag cagcaccgag gcgatcccgg ccgccttctc ggccagcttg ccggcgaaca
     9181 cgaggttgcg gtcgcgaagc gcctcgtcgg cctgctcgat gaaccgcttg gccatgtcgt
     9241 actgcccctg gccgtcctgg ctgagggcgc cgtaatccac ccggttgagg tcgctcgacg
     9301 cccgctggag ttgcaggcgg acccggcgct cgacctctcc cggcgaagtg gacatcaccg
     9361 gctcgagcga ctcggcgggc gaggcaacgg gcggcacggc cggcgcttcg gcggagggag
     9421 gcggcgcctc gggcttggcc gtcctcgact cgggccgggg caccgaacgg cgcgtgggcc
     9481 gcgaggtcag ctcgctgggc tcgggaagcg gctcgagcgg aatcggttcg ggctcgggcg
     9541 gcacgatgac gcggggcggc gcgggcggca ccagcagcgg cgtcgcgtcg ggctgcagct
     9601 gcggccgcga cttcgcgcag ccggccacca gcagcagcgc cacgagcgcg gtcccggtcc
     9661 atcctcgcat cggcttcatt atacggcccc tcgcgaaagc cgccgcgcac cgccaggccg
     9721 ttgaagaacg cggcctgaag cccctcaaca cgcggctacg cctgtcgcag cacgatccgg
     9781 aacgtcgtgc cccgcccctc ggtcgactcg acctcgatct cgccgtcgtg catctgcacg
     9841 atgcggtaca ccatcgagag gccgatcccg ctcccgcggt cgcgcgtggt gaagtacagg
     9901 tcgaagattc gctccaggtg ctcgggcttg atgccgatgc cggtgtcctc gaatcgcacc
     9961 tccacgcgcc ggcccgggag cggtgaggcc gcgatccgca gctgcccgcc gttgggcatg
    10021 gcctggcagg cgttgagcgc gaggttgagg aacgcctgcc gcagcatcgt cgagtcgccg
    10081 ctcaccggcg ggaggctgtc gaagccggtg accgtgaggg ccacgcccga cttggcgcac
    10141 tcgggctcga cgatccgcac cacctcgtcg acgaccgagc cgagcgacag gtcctgcagc
    10201 ttcaggtcct cggcccgcgt gaacttcagg aagccctgga cgacctggtc gaggcgctgg
    10261 atctcgctgc cgatgatctt cacgtgatcc agggcggccg agaggtcggg cccgttaacc
    10321 tcgaccggat cgggcgtcga gaggccgagc cccgaggcgg gcgccggcac ggccaccgcg
    10381 gcgcgcgggc gccggcggac ggtggtgggc gagagcttct gcttgagcag ctcgaggtgg
    10441 atggtcatcg cgttgagcgg gttcttcacc tcgtgcgcca cgcccgccga cagccgcccg
    10501 agcgccacca gcttccgcga gtagttcagc atcgactgga cgcggcccat gtactcgacg
    10561 tcgcgcgaca ccagcatcac gccgaccagc tgctggtcga ggtccttgat ggcgtgcgcc
    10621 atcacgaggc gctcggtcgc cggcggggct gcctcgtctc cctcgggggc ggcggcaccg
    10681 ggaggcagcg ggcccgggat ggacgccgac acggggcccc gcgactgccc ctccctgagg
    10741 gtcgcctgca ccagctcgcg gtaggggtgc ccagcaggca gcacgtcgtc gatggggcgt
    10801 ccgaacgact cggggggcag cgcacgccgc atcgcggggt tggcgaagag caactcgccg
    10861 gccgggttga tgagggcgac ctcgtcctcg atgtgttcga cggccgcctc gaggttggcc
    10921 ttctggccgg ccagctgcga ccggtcggcc gagagctgcg cgctgaccgc gttgaaaaag
    10981 ttgcccagct ccccgaactc gtcctgctgg ggcaggtcga gcttgacgcc gaactccccg
    11041 cggccgagac gggtgaggcc gccgcggatg acgtgaatcg gccgcagcat caggcgcgtg
    11101 aacacgccgg cgacgaggat cgccagtgcg agcgcgatca gcgcggtcac catggcgggc
    11161 ttgagcgcct cccacagctc ctgccggatc aggagcgtcg agacgcccac ccggatcgcg
    11221 ccgaagtccc tcgtaccgag gcgcagcggc tggcccacct cgagtgtgag gctctccgag
    11281 gaatacaccc tcgccacctg ctggcgcctc ggcaacgcga gcacctcgtc gaggcctccc
    11341 gcgggcgcga gcggcttccc ctggcggtcg ggcaccgagt gcaccacggc cacgccgtcc
    11401 gggtcgacga tggccgcgta gacgaccgac ttcgagtagg cgctcgactc gaggatcgag
    11461 cggaggccgc cgtcctgccg cagggcctcg tacgggtcgg cctgccccgg caccacgagg
    11521 cgggcgcgct ggaagatcgc ggccactagc aggtcggcgc gtgccccgct ctcctcgagg
    11581 ctgatcttga ccagcgtcga caggtggatc gcgctcagcg cgacgacggc gaggccgacg
    11641 atcagcgaga taccgccgat ctgcttggcc ttgatggaaa ggtgcaagga actactttct
    11701 cagcttcagc ttgttcagct tgttgtgcag cgtcttcagg ctgatgccaa ggatctcggc
    11761 cgcacgcgtc ttgttgtcgc gggtgtgctc gagcgtcatc tggatcagcc gccgctccgc
    11821 ctcctcgacc gtcgtccccg gcggcagctg cagcgagggc ctcgccgtct cgccggcctg
    11881 gacgagcgcc gccggcaggt ggcggggctc gatgaactgc ccgtcggtca ggatcgtcgc
    11941 ccgctcgatg acgttacgca gctcgcggac gttgcccggc cacccgtagc cgtcgaggat
    12001 acgcatcgcg tcgttgttca gcgcggcgat cgacttgccg ttgcgctggt tgaactccga
    12061 aaggaagaac tgagtcagca gcggcaggtc gtccttgcgg tcgcgcagcg ggggcagcgt
    12121 gatgccgaag acgttgagcc ggtagtacag gtcctcgcgc agcttcccct tcttcacggc
    12181 ctcgtccgga tcggcattgg ttgccgcgat cacccggacg tcgaccttct gctcctggcg
    12241 tccgcccagg cggcggaagc tgcgctcctg cagcacccgc agcagcttca cctgcgtcgc
    12301 cggcgtcatc tccgcaattt cgtcgaggaa gatcgtgccc cggtcggcca gctcgaagca
    12361 gccctggcgg cggtcggcgg ccccggtgaa ggcccccttc tcgtggccga agatctcgct
    12421 ctcgagcagc gtctctggga tcgcggcaca gttgatcgcc acgaacggcg cgttcgcgcg
    12481 cgggctcagc tggtggatcg tttgtgccac cagctccttg ccggtccccg actcgccgct
    12541 gatgaggacc gacgccgagg tcggcgcggc ctgctcgatg acgcggtaca ccttccgcat
    12601 gccagggctc gcgccgatca tgcggccgaa cgccccctgg tcgcgcagct gccgtctgag
    12661 cgccttcacc tcgcgcagcg tctcctggcg ctcgacgacg ttggcgagca ggatctgcag
    12721 gcgctgcgga tcgaccggct tggtcaggta gtcgtaggcg ccgtccttga tcgcttcgac
    12781 cgccgactcc acagatccct gtgcggtgag gatgacgacg gcgatgtcgg ggtcttgctc
    12841 gcgcagcgcc ttgagcagct ggtggccgtc catgcggggc atcaccaggt cggtcacgac
    12901 gatcgccggg cggaaggagg tgatcttctg aagcccctcc tcgccgtcct cggcgctgtc
    12961 ggccaggtag ccccagttcc tcaccagctc ggtgagcccc aggcgcgtgg acgggtcgtc
    13021 ctcgacaatc agcacccgct cggtgacgcc cggggcctcc ccggactccg cgtcggctgc
    13081 ggcgggatga acgtgttcca cgggaatggc ctcggaaaga acgccggccg gcggccggca
    13141 cgagtggtcg gtaaaaaccc agtgtatcgg gtaattctta cacggtccag ctgagcggga
    13201 gcggaatgcg ggcgctccgg aagatcgggc attttccgcc gagatcgccc tggcaccgtt
    13261 cgccgcagcg ccggcgcacc ttccaaccaa cagctcgtct gcaggattcg atccggcccg
    13321 tgatagagtc ggcccgtgga cgacgattcg ctcgcgcagg ggctgccgcc ccctctgcgc
    13381 gacccctcgg ggggcctgaa tgcccgggcg gcggcggtca tcgaagtgat cctctgctcg
    13441 gggctcccca cccagctggc tctcgcggga ctcctggccc tcgcgggcat cgcgccgtac
    13501 ggccccgacg gggccctttc ccccgcctac gtgttcgtgc tgccgctcgc cgacgccgtt
    13561 ctcctgctgg ggctggtgac gtggttcctg cggctgcacg gcgaacgggt gtcggatgtg
    13621 atgctcgggg gacggccgct cctgcgcgag gcggccgtcc ggggttcgct cggcgtcgag
    13681 gggcgtgaca tcggcacggt cgtcttcccg gacgcggtgc tcaaggtgca cctgcacgca
    13741 gcgctcccgg tccgcgccga gcgccggcag gcggacctgc gccggcaggg gatcgacatg
    13801 cccgccgggg aggtctcgcg cgacctcgag gagcgcgacc ggcgcgactc gacccgggcc
    13861 gactcgccgt tgcgccaggc cgagggggcg ctcgtgctcg acacgagccg ttgctcgatc
    13921 gacgagcagg tcgacgcgat cctgcgcgcg tacttcgccg ccgtgcgcgc gacgcgcgcg
    13981 ccggaggggc cggccgggcc ggaggcctcg cggccgccgg aaggggaacg gccctgaagc
    14041 gccgccgccc gcactacgtg ttcggacagg cggtcgtgcg cgcctacgcg catgtcgcgt
    14101 tcgggctgcg cgcgaccggg ctcgagcacg tcccggccg
//
LOCUS       DAOPQX010000059        13949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5130_length_13949_cov_5.040737, whole genome shotgun sequence.
ACCESSION   DAOPQX010000059 DAOPQX010000000
VERSION     DAOPQX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5130_length_13949_cov_5.040737"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..638
                     /locus_tag="PKK95_05010"
     CDS             177..638
                     /locus_tag="PKK95_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOC17605.1"
                     /translation="MRVGLISDTHGLVRPEVLLAFEGVDRILHAGDVGGGDVLAILAR
                     VAPVEAVFGNVDVPGDPRLTDHLYDRIGGVSVHVSHGHELGSPTPARLLARYDADVLV
                     YGHTHKPLIQRAAARLVVNPGAAGPRRFDIQPSVAILTIRSGRAEAELIPL"
     gene            complement(661..2358)
                     /gene="ggt"
                     /locus_tag="PKK95_05015"
     CDS             complement(661..2358)
                     /gene="ggt"
                     /locus_tag="PKK95_05015"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOC17606.1"
                     /translation="MRRRRSWILLALFLAIPAAATAQQPVRARDGLVVSQNETASRVG
                     AAVLKEGGSAIDAAIATAFALAVTHPTAGNIGGGGFIVFRPASGAAEAYDFRETAPRR
                     ASPTMFLEDGKYSERLHHDSHLSVGVPGTVAGLHLAWSEHGKLPWKRLVEPAIALARD
                     GFTVTDGLARSLRGILPDMKAYPASVAQFSKNGVPYEPGETLRQPDLARTLQRIAERG
                     PAGFYEGETAALIEKEMAANGGLIDREDLKAYRPRKRVPVRGTYRGFEIISMPPISSG
                     GVALIQQLNVLEGYDLKSLGFRSAASIHLIAEAMRRAYADRARYLGDPDFNPGMPVSR
                     LISKEYAAELRRSISAERASVSSPTRFDWPAESDETTHLSVVDGDRNAVALTYTLEYG
                     YGSRIVVPGAGFLLNNEMGDFNAGPGLTTADGLIGTEPNLARPRKRMLSSMTPTIVAR
                     DGKLFMVTGSPGGRTIINTVLHTILNVIDYGMGAQEAVDAPRFHHQWLPDRITYERWG
                     ISPDTAALLQAKGHRLNSIRSQGVAEVIVVNQAENLLEGGVDRRAPDGGAAGVDKRR"
     gene            2478..4136
                     /locus_tag="PKK95_05020"
     CDS             2478..4136
                     /locus_tag="PKK95_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC17607.1"
                     /translation="MCAITSVAGCGGPKGTAPNAELVIINARVWTVDPDRSEAQAVAV
                     RDGRISAVGSTDEVRRLIGPATRVIDAGGRFLMPGFHDSHIHLMMGGHQLEAVDLKDA
                     PTPEEFARRIGERAKAVPKGEWILGGNWDEQGWPGAPLPTRQMIDGVTPDTPVFVQRY
                     DGHMGLANTAALKLAGVTAATPEGPGGQIVRDARGEPTGILKDAAMALVQRVIPDETA
                     EQRLRTLEHALGHMASLGVTSVQDMGPDPEDVRLYAQLAGQGRLTSRIRAVRAEVPLA
                     RELASGQATVAATGSPFLFVSGAKGFADGSLGSTTAFFFEPYTDDPSSSGLLSDEMQP
                     IEGMRERLVALDRAGQQLCIHAIGDRAISMVLDLFDEVATANGPRDRRPRIEHSQHVA
                     PGDFARYAALGVIASVQPFHAIDDGKWAERRIGPARVKTTYAFKSFLDAGARLAIGTD
                     WPVAPLDPSLNIYAAVTRATLDGKNPGGWVPEQKLTVAQVIEGYTLGAAYAAFEEREK
                     GSVTVGKLADLVLFDRDPFSVAPEALRDLKVDLTITGGRVVYER"
     gene            complement(4133..5473)
                     /gene="thrC"
                     /locus_tag="PKK95_05025"
     CDS             complement(4133..5473)
                     /gene="thrC"
                     /locus_tag="PKK95_05025"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOC17608.1"
                     /translation="MENSRPARTRSFGEAVLDGLAPGGGLYLPSGFTTVPATEIASWR
                     GFPIAEIAVRVLARLLGDEFGSARLEPLTRDAFSFASRLVPTEDGISVLELFHGPTLA
                     FKDFGARFLGRVFGALLAERGDHATILVATSGDTGSAVAHGFHAVPNLQVVVLYPDGK
                     VSRFQEAQMATLGGNVRALKVPGVFDDCQRLVKTAFCDESLASLHLSSANSINVGRLL
                     PQSVYYFHALLSVPHEAAGPSLLPETIFSVPSGNLGNLTAGVIAQRMGLPAARFLAAC
                     NVNDVLPEFLRTGTYRARPSVATASNAMDVGNPSNFVRLAALHGDSMEEMRANLAGYR
                     VDEAETRATIREVYRRTGYVLDPHGAVGYAAALRYRAETGDRRPIVTLATAHPAKFSD
                     VLREELGFEPALPAEYTGWESRPILAQALSDARYETFKEWLEGLKAGGPANHRA"
     gene            complement(5509..6462)
                     /locus_tag="PKK95_05030"
     CDS             complement(5509..6462)
                     /locus_tag="PKK95_05030"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOC17609.1"
                     /translation="MQRRVRVFAPASVSNLGPGFDVLGLAIDRPGDIVEAEFRDGPGV
                     EIAAVTGDGGLLPVDPERNVAGVAASSVLAHLAACGAAPPAGVRLWVHKQMPLASGLG
                     CSAASSVAGALAVNELFGRPLPKEALLTHALAGEKVAAGTVHADNVAPSLLGGIKAIR
                     GYDPLEVLDLPVPPALRLVVVHPRTEVQTSYARRVLREHGFPIEMAVANLGNMAGFVA
                     ALFRGDLELLGRSVEDRLAEPLRVALIPGAAAVKLAAREAGALGCSISGSGPSVFAVT
                     ADDETAGRVAAAMRAAFIEEAGLESEAFTGPVNAEGARRVL"
     gene            6589..7071
                     /locus_tag="PKK95_05035"
     CDS             6589..7071
                     /locus_tag="PKK95_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC17610.1"
                     /translation="MVRNPYEDDLGNRDPLEAMSETPGRIRAVVSTLSPEDLERSHAP
                     GKWTLRQLLVHLAQTELALPVRARMALTQKGYVAQPFDQNDWMAAESRVDGTTALAAY
                     TAVRQMNLQFFSALSASERATGFHHPEYGEITIDWLMRQIAGHELHHLRHFEAVAGQR
                     "
     gene            7110..8693
                     /locus_tag="PKK95_05040"
     CDS             7110..8693
                     /locus_tag="PKK95_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOC17611.1"
                     /translation="MAARIGRQRRGCLLGLLVVLFLLAGLAFAVYRTLDPASLRSLAE
                     ARLSAALGQKVTIGTIGIDWLPTPSVEAREITLAATEGQAPPSVALGSIRIVPRFSSL
                     FSRPIVAERVELVGVVVHALRDRDGRWQLPFPAMPPTGGTNGSPSLDVGEVVIQEGRL
                     SVSDAGRNDRGQPPSIRGLAATVRRHGAQTRLDNLSGAIGQSSLSGSGTLGPEGLRLS
                     LAWNRLRPADLPEVFGLAGVEMPRGVSIEGDKPLTLDVTIDKSGAIAASGEMAAAKAG
                     FQGLALTSLKSPVRLAANQLTLDPVTFDVYGGRQSGRATANLGATPLAWTLDTRIEGV
                     DVRRLVNATTSVPDSLSGVGALRGRLEGVMVEPVLDRAAGTMTVAISNGAIHNFPLIA
                     AVNSALRIAEGDERDLRFESLTATMAIAGGRAETRDLLVRAGELTVVGSGTLGFDRSL
                     DFHGHVVFSAKKAAELVRAVREAARLRNAKGEIDVPVVVSGTVTDPHFGVDTAALLQR
                     GLQDELERRLKKGLQGIIK"
     gene            complement(8686..9786)
                     /locus_tag="PKK95_05045"
     CDS             complement(8686..9786)
                     /locus_tag="PKK95_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOC17612.1"
                     /translation="MRLFGWLAAVSVGLATVGAAAMHRQATAVSAAPNGPVKVAEFIN
                     DDAPYPECHASTIVEASPGRLVAAWFGGTREGAADVSIWVARHDGTRWLPAKAVADGR
                     QEDGTRHPTWNPVLFKPAAGPLFLFYKVGPSPREWWGMVRTSADGGATWSEARRLAAP
                     LLGPIKNKPVVLADGTWLSASSTEGTPTGWRAHFEASRDGGATWQFAGPIDKGPESLE
                     AIQGSVLFIGGDRLLALLRTRNGVLAATRSADNGRTWSPLAPSGLPNPNSGTDALTLR
                     DGRHLLVFNNSRPPAGRTSGPRYPLDVALSGDGERWDVALTLESEPRPHGYAYPAVIQ
                     AADGLVHITYTWNRQRIKHVVIDPAALGRQPT"
     gene            complement(9847..11154)
                     /gene="citX"
                     /locus_tag="PKK95_05050"
     CDS             complement(9847..11154)
                     /gene="citX"
                     /locus_tag="PKK95_05050"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HOC17613.1"
                     /translation="MDLEGLRRSLLDARDARAALLGGVASRPGRTTLVFLSLNVPGPD
                     KMTPRLGWVFARARVALSAELPGSSLLAEGRDALGPYALLKVPITAANAKVACVRLEE
                     SLPFGRLLDLDVLAPGGAQVDRASLGLPPRRCLVCDRAAVECIRTARHSSAELSSAAL
                     AVARSPLVALARALADGARAELDLTPKPGLVDRLDSGSHPDLSHASMRRSIDTLPAYY
                     QDLIELADGEPLDLAACVSAGVRAEERALAAAGSNAHRGYIFLSGLLLLAAAADQTGT
                     TAGWRAETRAVARRLPGEATGAEPSHGEQARRGYRVEGVLGEAAAGLPSVFDHGLPAL
                     DDALARGLSPETAQHYAMAVLMTRVEDTTALHRCGPAGLARLKEDGASLAALIEEGGD
                     HLRRLRALNDDYRAMRLTMGGVADCLALTLAVRTMVALSGAPG"
     gene            complement(11164..12702)
                     /gene="citF"
                     /locus_tag="PKK95_05055"
     CDS             complement(11164..12702)
                     /gene="citF"
                     /locus_tag="PKK95_05055"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HOC17614.1"
                     /translation="MARNSLGREVPERWNGRCYEPYRDPWSRMPAVNRATRPLVRRNP
                     GESKVLPSLRAAIERSGLRDGMTIATHHHLRNGDALLNLVVAELDAMGFRDIAIASSS
                     VHPVHAAIVPSIRKGVVTRCETGVNGPIGELASRGELDFPIVVRSHGGRARSLVTDEV
                     VVDVAFIAAPCCDETGNLNGVSGPSACGSLGYAFTDARYARKVIAVTDHLVPYPASPV
                     SISQSDVDYVVKIDSLGDPKKIVSTTTRVTRDPAGLLIAHYAAEVIEAAGLLRDGFSF
                     QTGAGGTSLAVADAVKTKMLAGSVKGSFGCGGITGYFVDMLEEGYFRTLFDVQCFDLR
                     AVESIGRNLMHREISADLYANPFNRGSVVNMLDCVILGATEVDVNFNVNVNTESNGLL
                     LHNTGGHCDTAAGAKLSIVVAPAIRGRLPIVRDEVTTITTPGETIDVVVTERGIAVAD
                     RHPDLKKELVRRRLPLKDIRELQAEICALTGTPRPLEFEDRIVALIEYRDGSIIDAVR
                     QVKD"
     gene            complement(12689..13615)
                     /locus_tag="PKK95_05060"
     CDS             complement(12689..13615)
                     /locus_tag="PKK95_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOC17615.1"
                     /translation="MIDRRQRLRRSLLYVPGNMPGMLQNIPVFDADGVMIDLEDAVPL
                     AEKDSARILARSFLQAFPKRSREIFVRINGLDTKYALDDLREVLPARPDGIRLPKADD
                     PEVVERLDTLLTEYEEKLGLDIGFFSIVPSIESAVGVLNCVKTATASHRIVALAFGAE
                     DYTASMEIQRTRTGDELLGARMQIVWAARAAGLQAIDSVFADANDMEGLRADTELVKR
                     LGFTGKSLVNPRQIAVVHEVFRPTGEEIEHALEVMEAIKKAREMGTGVISLRGRMVDA
                     PVVKRAARTIRTAVAFGMLDYELSDEVVHGQE"
     gene            complement(13618..>13949)
                     /locus_tag="PKK95_05065"
     CDS             complement(13618..>13949)
                     /locus_tag="PKK95_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOC17616.1"
                     /translation="GSIRDVEFTDIQAGARLPITEPRMPVHRLEGPLDLLGLEGNLVP
                     NEEGRVDAHLHVFAARSSGEAVGGHLRDAEVFATCEIILGEYIVEGVERQHSMRGGVD
                     TLFFEDQ"
BASE COUNT         2028 a         4882 c         4980 g         2059 t
ORIGIN      
        1 cgtgttcctg gtggacatgt ccgatttccc cgcgatgaac gcggtctacg cgcgggtctt
       61 cggggatcac cggccggcgc gcaccaccgt gcaggtggcc gcgctgccgc accccgagct
      121 gcgcgtcgaa atcgacgtca ttgcctacct cccctgaccg ggttcgaagc cgggcggtgc
      181 gtgtcgggtt gatctccgac acgcacggcc tggtgcggcc cgaggtgctg ctggcctttg
      241 agggagtcga ccggatcctg cacgccggcg acgtgggcgg tggcgacgtg ctcgcgatcc
      301 tcgcgagggt ggcgccagtc gaggcggtgt tcggaaacgt ggacgtgccg ggcgaccccc
      361 ggctcaccga tcacctctac gatcgaatcg gcggggtgag cgtgcacgtg agccacggcc
      421 acgagcttgg cagcccgacg cccgcccggt tgctggcgcg ctacgacgcc gatgtcctgg
      481 tgtacgggca cacccacaag ccgctgatcc agcgcgccgc ggcccgtctc gtggtgaacc
      541 ctggcgcagc gggaccgcgc cgcttcgaca tccagccgag cgtggccatc ctgaccatcc
      601 ggagcgggcg ggcagaagcg gaactgattc cgctgtgacc tggcggctgt ccggtgcccg
      661 ctaccgcctc ttgtcgactc ccgcggcgcc gccgtctggc gcccgccggt cgacgccccc
      721 ctcgagcagg ttctccgcct ggttgacgac gatgacctcg gcgacgcctt gagagcggat
      781 tgagttcagg cggtgtcctt tcgcctgcag cagggcggcg gtgtcgggcg agatccccca
      841 cctctcgtag gtgatccggt cggggagcca ctggtggtgg aagcggggcg catcgacggc
      901 ttcctgcgct cccatcccgt agtcgatcac gttgaggatc gtgtggagca cggtgttgat
      961 gatcgtgcgg ccgcccgggc tgccggtcac catgaagagc ttcccgtcgc gcgccacgat
     1021 ggtcggcgtc atgctggaca gcatgcgctt gcgcggtctc gccaggttcg gctccgtccc
     1081 gatcaggccg tccgccgtcg tcagccccgg cccggcgttg aagtcgccca tctcgttgtt
     1141 caggaggaag cccgccccgg gtaccacgat ccgcgagccg tagccgtact cgagggtgta
     1201 agtcagcgcc acggcattgc gatcgccgtc caccaccgac aagtgggtgg tctcgtcgct
     1261 ctccgccggc caatcgaagc gggtgggcga tgagaccgat gcccgctcgg ccgaaatcga
     1321 ccgccgcagc tcggccgcgt actccttcga gatcagccgc gagaccggca ttccagggtt
     1381 gaagtcggga tcgccgaggt agcgggcgcg gtcggcgtag gcgcggcgca tcgcctcggc
     1441 gatgagatgg atgctggcgg ccgaacggaa gcccaacgac ttcaggtcgt atccctcgag
     1501 gacgttcagc tgctggatga gggccacgcc gcccgagctg ataggcggca tggagatgat
     1561 ctcgaagccg cggtacgtcc cccgcaccgg gacccgcttc ctcggccggt aggccttcag
     1621 gtcttcgcgg tcgatcagcc ctccgttcgc ggccatctcc ttctcgatga gcgccgcggt
     1681 ttccccctcg tagaacccgg ccgggccgcg ctcggcgatg cgctggagcg tgcgcgcgag
     1741 gtcgggctgc cggagggttt cgcccggctc gtacgggacg ccgttcttcg agaactgcgc
     1801 gaccgaggcg gggtacgcct tcatgtcggg caggatgccg cgcagcgagc gggcgaggcc
     1861 gtcggtcacg gtgaagccgt cgcgcgcgag ggcgatggcc ggctccacga gccgcttcca
     1921 gggcagcttg ccgtgctcgc tccaggcaag gtgcagcccc gccacggtgc cgggcacgcc
     1981 gaccgacagg tggctgtcgt ggtggagccg ctcggagtat ttcccgtctt cgaggaacat
     2041 cgtcggcgag gcccgccggg gagcggtttc acggaagtcg taggcctcgg cagcgccgga
     2101 cgccggtcga aagacgatga agccgccgcc gccgatgttg cccgcggtcg gatgggtgac
     2161 ggcaagcgcg aacgccgtgg cgatggcggc atcgatggcc gagccgccct ccttcagaac
     2221 cgctgccccc acgcgcgaag ccgtctcgtt ctgcgagacg accaggccgt cccgcgcgcg
     2281 caccggctgc tgcgcagtgg ctgcggcagg gatcgcgaga aacagggcga gcagaatcca
     2341 gctacggcgc cggcgcatcg ggttcctccg ccgcggatta taatccgttg ccgtcgcccc
     2401 tacgtctcct gaggtgtcac atggccaggc gttctcacgc tcgttcgacg gactggctgg
     2461 tgcgagggtt gacgatcatg tgtgcgatca cgtcggtcgc gggctgcggc ggtccgaagg
     2521 ggacggcgcc gaacgccgag ctggtcatca tcaacgctcg agtgtggacg gtcgaccccg
     2581 atcggtccga ggcgcaggcg gtggccgtgc gcgacgggcg catctcggcg gtgggcagca
     2641 ccgacgaggt gcggcgcctg atcgggccgg cgacacgcgt catcgacgcc ggcggccggt
     2701 tcctgatgcc gggcttccac gactcgcaca tccacctgat gatgggtggg caccagctcg
     2761 aggccgtgga cttgaaggac gccccgacac ccgaggagtt cgcgcgtcgc atcggcgaga
     2821 gggccaaggc cgtgccgaag ggggagtgga tcctcggcgg caactgggac gagcagggct
     2881 ggccgggcgc cccgctgccc acgcggcaga tgatcgacgg cgtgacgccc gacacccccg
     2941 tgttcgtcca acgctacgac gggcacatgg gactcgccaa cacggccgcg ctgaagctgg
     3001 ccggcgtgac cgcggcgacg cctgaaggac cgggaggaca gatcgtccgc gacgcgcggg
     3061 gggagccgac cgggatcctg aaggatgcgg cgatggctct cgtgcagcgg gtgatccccg
     3121 acgagacggc cgagcagcgc cttcggaccc tcgagcacgc gctcggccac atggcctcgc
     3181 tcggcgtgac gagcgtgcag gacatgggcc ccgatcccga ggacgtccgg ctctacgcgc
     3241 agctcgccgg ccaggggcgg ctgacctcgc ggatccgggc ggtcagggcc gaggtgccgc
     3301 tggcgaggga gctggcgtcc gggcaggcca ctgtggcggc gacgggctcg ccgttcctgt
     3361 ttgtgtcggg tgccaagggg ttcgccgacg gctccctcgg ctcgaccacc gccttcttct
     3421 tcgagcccta cacggacgac ccgtcgagca gcggtctcct gtccgacgag atgcagccga
     3481 tcgaggggat gcgcgaacgg ctcgtcgcgc tcgaccgcgc gggccagcag ctctgcatcc
     3541 acgcaatcgg cgaccgcgcg atctccatgg tgctcgacct gttcgacgag gtggcgacag
     3601 ccaacggccc cagggaccga cggccgcgga tcgagcactc gcagcacgtg gcgcccggcg
     3661 acttcgcccg ctacgcggcc ctgggcgtga tcgcctcggt gcagccgttc cacgccatcg
     3721 acgacggcaa gtgggccgag aggcggatcg gcccggcgcg cgtgaagacg acctacgcgt
     3781 tcaagtcgtt cctcgatgcc ggcgcgaggc tggcgatcgg caccgactgg ccggtggcgc
     3841 cgctcgaccc gagcttgaac atctacgccg cggtcacgcg cgccacgctc gacggcaaga
     3901 acccgggcgg gtgggtcccg gagcagaaac tgacggtggc gcaggtgatc gagggataca
     3961 cgctgggggc ggcctatgcc gccttcgagg agcgcgagaa gggatccgtc accgtcggca
     4021 agctcgccga cctcgtgctg ttcgatcgcg acccgttctc ggtcgcgccc gaggcgctgc
     4081 gcgacctgaa ggtggacctc acgatcacgg gcggccgggt cgtctacgaa cgctaggccc
     4141 tgtggttcgc tggccccccg gcctttagcc cctccaacca ttccttgaac gtttcgtacc
     4201 gggcgtcgga gagcgcctgc gcaagtattg ggcgcgattc ccatcccgtg tactcggcgg
     4261 gcagggcggg ttcgaagccc agctcctcgc gaagcacgtc ggaaaacttt gccgggtgcg
     4321 ccgttgccag cgtcacgatg ggtcgacgat cccctgtttc ggcgcggtag cgcagggcgg
     4381 ccgcgtaacc gactgcgccg tgcggatcga gcacgtagcc cgtgcggcgg tagacctccc
     4441 ggatcgttgc ccgcgtctcg gcctcgtcca cgcggtagcc cgccaggttc gccctcatct
     4501 cctccatcga gtcgccgtgc agcgccgcca ggcgcacgaa gttgcttggg ttgccgacgt
     4561 ccatcgcgtt cgaggcggtg gcgaccgagg gcctggccct gtacgtgccg gtacggagga
     4621 actcgggcag cacgtcgttc acgttgcagg cggcgaggaa tcgggcagct ggcagcccca
     4681 tccgctgcgc gatgacgccg gctgtcaggt tcccaaggtt tccgctgggc accgagaaga
     4741 tcgtctcggg gagcaacgag gggccggctg cttcgtgagg aacagagagg agggcgtgga
     4801 agtagtagac cgattgcgga agcagccgcc cgacgttgat cgagttcgcc gatgaaaggt
     4861 gaagcgaggc caggctctcg tcgcagaacg cggtcttcac gaggcgctgg cagtcgtcga
     4921 agacgccggg caccttcagc gcccgcacgt tcccgccgag ggtggccatc tgcgcttcct
     4981 ggaagcgcga caccttgccg tcggggtaga gcacgacgac ctggaggttg ggcacggcgt
     5041 ggaaaccgtg ggcaaccgcg ctgcccgtgt ctcccgaggt cgcgacgagg atcgtcgcgt
     5101 gatcgccgcg ctccgcgagc agcgcgccga agactcggcc gaggaaccgg gcgccgaagt
     5161 ccttgaaggc gagcgtcggc ccgtggaaca gctcgagcac gctgatgccg tcctccgtcg
     5221 ggacgagtcg cgaagcgaag ctgaaggcgt cgcgcgtcag tggttcgagg cgtgccgagc
     5281 cgaactcgtc gccgagcagg cgcgccagca cgcgcacggc gatctcggcg atggggaagc
     5341 cccgccacga ggcgatctcc gttgccggga cggtggtgaa tccggacggc aagtacaggc
     5401 cgccgccagg cgcaaggccg tcgagaaccg cctcgccgaa cgaacgggtg cgagcgggcc
     5461 gactgttctc caagggttca ggaccgggtc ccacggcatg gaggcgcatc aaagtacccg
     5521 ccttgcccct tcggcgttga ccgggccggt gaacgcctcg ctctcgaggc cggcctcctc
     5581 gataaacgcg gcgcgcatgg ccgccgccac ccgcccggcc gtctcgtcgt ctgccgtcac
     5641 ggcgaacacc gaggggcccg acccggagat cgagcagccg agggcgccgg cctcgcgggc
     5701 cgccagcttc acggccgcgg cgccggggat gagcgccacc cgcagcggct cggccaggcg
     5761 atcctcgacc gagcggccga gcagctcgag atcgccgcgg aagagggccg cgacgaagcc
     5821 agccatgtta cccaggttgg cgaccgccat ctcgatcgga aagccgtgct cgcggaggac
     5881 ccggcgcgcg tacgacgtct gcacctcggt ccgcgggtgc acgacgacga ggcggagggc
     5941 tggcggaacc ggcaggtcga gcacctcgag cggatcgtag ccgcggatcg ctttgatgcc
     6001 gccgagaagg cttggcgcca cgttgtcggc atggacggtt cccgccgcca ccttctcccc
     6061 ggcgagcgcg tgtgtcagca gcgcttcttt tgggagcggg cgcccgaaca gctcgttgac
     6121 ggccagcgcg ccggcgacgc tgcttgccgc cgagcagccg aggccgcttg ccaggggcat
     6181 ctgcttgtgc acccagagcc ggacgccggc cggaggcgcc gccccgcagg ccgccaggtg
     6241 cgcgagcacg gatgaagccg cgacgcccgc gacgttccgc tcgggatcca ccggcaggag
     6301 cccgccgtca cccgtgacag cggcaatctc cacgcccggg ccatcccgga actcggcctc
     6361 gacgatgtcg ccgggacggt cgatggccag gccgagcacg tcgaaacctg gcccgaggtt
     6421 gctgaccgag gcgggcgcga acacgcgaac gcggcgctgc attccgccat aataggactt
     6481 tggtcacgaa aaccggagaa cggaacacgg agaatgcggg cgggccggcg ccttgggcgg
     6541 agcgaggggc gcgagcgcaa gcggcggccg cacgagatcg gaggcgccat ggtccggaac
     6601 ccttacgaag acgacctcgg caaccgtgat ccactcgagg cgatgagcga gacgccgggt
     6661 cgcattcgcg cggtcgtctc gacgctgtcg cccgaggatc tcgagcgcag ccacgcccct
     6721 gggaaatgga cgctgagaca gctgctcgtc cacctcgccc agacggagct ggcgcttccc
     6781 gtccgggcgc gcatggcgct cacgcagaag gggtacgtcg cgcagccgtt cgaccagaac
     6841 gactggatgg ccgccgagtc gcgcgtggac ggcacgacgg cgcttgccgc atacacggcc
     6901 gtgcgccaaa tgaacctgca gttcttctcg gcgctgtcgg cgtccgagcg cgcgaccggc
     6961 ttccatcacc cggaatacgg cgagatcacc atcgactggc tgatgcggca gattgccggc
     7021 cacgagctgc accacctgcg gcatttcgag gcggtcgccg ggcagcgttg aggatcgacc
     7081 cggtcggccc ccggaagcga ggcatcgcca tggctgcacg gatcggtcgt cagcggcggg
     7141 gctgcctgct cggcctcctc gtcgtgctct tcttgctcgc cggccttgcg ttcgccgtgt
     7201 acaggaccct cgaccccgcc tccctccggt cgctcgccga ggcgaggctg agcgccgcgc
     7261 tcggccagaa ggtcaccatc gggaccatcg gcatcgactg gctgccgacg ccgtcggtcg
     7321 aggcgcgcga gattacgctc gcggcgaccg aggggcaggc tccaccgtcg gtggccctcg
     7381 gctcgatccg gatcgttccg cggttctcgt cactcttctc ccgtccgatc gtcgccgaac
     7441 gggtcgagtt ggtcggcgtg gtggtgcacg cgctgcgcga ccgggacggt cgctggcagc
     7501 tcccgttccc cgccatgccc ccgaccgggg ggacgaacgg cagcccgtcg ctcgacgtcg
     7561 gggaagtcgt catccaagag ggccggctga gcgtgagcga cgcgggccga aacgaccggg
     7621 gacagcctcc ctccattcgc ggcctggcgg cgaccgtgcg gcgccacggt gcgcagacgc
     7681 gcctcgacaa cctgtcggga gcgatcggtc agtcctcgct gtcgggcagc ggcacgctcg
     7741 ggcccgaggg gttgcgcctt tctctcgcct ggaaccggct gcgccccgcc gaccttcccg
     7801 aggtgttcgg cctcgccggc gtcgagatgc cgcggggggt ctccatcgag ggtgacaagc
     7861 ccctcacgct cgacgtcacc atcgacaagt cgggcgcgat cgccgcgtcg ggggagatgg
     7921 ctgcggcgaa ggcggggttc cagggcctgg cgctcacatc gctgaagtcg cccgtgcggc
     7981 tggccgccaa ccagctgacg ctggatcccg tcaccttcga tgtctacggc gggcggcaga
     8041 gcgggcgcgc gaccgccaac ctgggcgcga cgccgttggc ctggacgctc gacacgcgca
     8101 tcgaaggggt cgatgtgcgc cggctggtga acgccaccac gtcggtgccc gacagtctca
     8161 gcggggttgg cgcgctccgc ggccggctgg agggagtgat ggtcgaaccc gtgctcgacc
     8221 gggcagcggg caccatgacc gtggcgatct cgaacggtgc gatccacaac ttcccgctga
     8281 tcgcggcggt caactcggcg ctgcggatcg ccgagggaga cgagcgcgat ctccgcttcg
     8341 agagcctgac ggcgaccatg gccatcgcgg gcgggcgcgc cgagacccgg gacctgctcg
     8401 tccgcgccgg ggagctgacg gtcgtgggct ccgggacgct cggcttcgat cgttcgctcg
     8461 actttcacgg ccacgtcgtg ttctcggcga agaaagccgc cgagctggtt cgggccgtcc
     8521 gcgaggcggc gcgcctgcga aacgcgaagg gggagatcga cgtgccggtc gtcgtatcgg
     8581 ggaccgtgac cgacccccac ttcggcgtgg acacggcggc gctgctccag cgcggcctgc
     8641 aggacgagct ggagcgccgc ctcaagaagg ggcttcaggg catcatcaag taggttgccg
     8701 ccccagagcg gccgggtcga tgacgacgtg cttgatgcgc tgcctgttcc aggtgtaggt
     8761 gatgtgcacg aggccgtcgg ctgcctggat gaccgcgggg tacgcgtagc cgtggggtct
     8821 cggttcgctc tcgagggtga gcgccacgtc ccaccgctcg ccgtcgcctg acagcgccac
     8881 gtcgagcggg tagcggggtc cggaggtgcg ccccgccggg ggccgcgagt tgttgaagac
     8941 gaggaggtgc cggccgtccc gtagcgtcag cgcgtcggtc ccggagttgg ggttcggcag
     9001 gccgctcggc gcgagcgggc tccaggtgcg gccgttgtcg gccgatcgcg tggcggcaag
     9061 gacgccgttc cgcgtgcgga ggagcgccag gaggcggtcg cccccgatga acagcacgct
     9121 cccctggatc gcctcgagcg actcggggcc cttgtcgatg gggccggcga actgccacgt
     9181 cgctccgccg tcgcgactcg cttcgaaatg agcgcgccag ccggtcggag tgccttcggt
     9241 gctcgacgcg ctgagccacg tgccatcggc gagcacgacc ggcttgttct tgatcggtcc
     9301 cagcaggggc gccgcgagcc tccgcgcctc gctccacgtc gccccgccgt cggcggaggt
     9361 cctgaccatg ccccaccact cgcgcggcga cggtcccact ttgtagaaca ggaacagcgg
     9421 gccagcggcc ggcttgaaga gcaccgggtt ccacgtcggg tggcgcgtgc cgtcctcctg
     9481 gcggccgtcg gccactgctt ttgccggcag ccagcgtgtg ccgtcgtgcc gggcgaccca
     9541 gatggagacg tcggccgccc cctcgcgggt accgccgaac cacgcggcaa ccagccgccc
     9601 cggcgaggcc tcgacgatcg tcgaggcgtg acactcggga tacggggcgt cgtcgttgat
     9661 gaactccgct accttaacgg gtccgttcgg cgcggcagac actgccgtcg cctggcggtg
     9721 cattgccgcc gcgccgacag tggccaaccc gaccgacacc gccgccagcc agccgaagag
     9781 tcgcatggcg acgatgatac cgtcaccggc gtgtgtcact cgctgtgtgg ccgtcatggt
     9841 ctgagcctag cccggcgcgc ccgaaagcgc caccattgtg cggacggcga gcgtgagcgc
     9901 caggcagtcc gccaccccgc ccatcgtcag ccgcatcgcc ctgtagtcgt cgttcagcgc
     9961 acgcagtctc cggaggtggt cgccgccttc ctcgatgagc gccgccaggc tggcgccgtc
    10021 ttccttcagc cgcgccaggc ccgccgggcc gcaccggtga agggcggtcg tatcctccac
    10081 cctcgtcatc aggaccgcca tcgcgtagtg ctgcgccgtc tccggcgaga gtccccgcgc
    10141 gagggcgtca tcgagcgccg ggaggccgtg gtcgaagacc gacggcaagc cggcggccgc
    10201 ctcgccgagg actccctcca cccggtaccc tcgccttgcc tgctctccgt gggaaggctc
    10261 agcgcccgtg gcctctcccg gcaggcgccg ggccacggcc cgggtctcgg ctcgccaccc
    10321 cgccgtcgtg ccggtttggt cggcggccgc ggcgagcagg aggagcccgg agaggaagat
    10381 gtagccgcgg tgagcgttgc tgccggccgc ggcgagcgcg cgttcctccg cgcggacacc
    10441 ggcagagacg caggccgcca ggtcgagagg ctccccgtcg gccagttcga tcaggtcctg
    10501 gtagtaggcc gggagcgtgt caatcgagcg gcgcatggac gcgtgcgaca ggtccggatg
    10561 cgagccgctg tcgagccgat ccaccagccc gggcttgggc gtcaggtcga gctcggcgcg
    10621 ggcgccgtcc gcgagcgcgc gggcgagggc gacgagcggc gagcgcgcca ccgcgagcgc
    10681 cgccgacgac agctcggcgg aggagtggcg cgcggtgcgg atgcactcca ccgcggcgcg
    10741 gtcgcagacg aggcacctgc gcgggggcag gccgagcgac gcccgatcca cctgcgcccc
    10801 tccgggcgcc aggacgtcga ggtcgagcag gcggccgaag ggcagcgatt cctcgagacg
    10861 gacacacgcc accttggcgt tggccgcggt gatcggcacc ttgagcagcg cgtaggggcc
    10921 cagcgcgtcg cggccttcgg cgaggaggct ggagccgggc agctcggcgg acagcgcgac
    10981 gcgagcccgg gcgaagaccc agcccaggcg gggcgtcatc ttgtcggggc cgggaacgtt
    11041 cagggagagg aagacgagcg tggttcgccc gggacgactc gccaccccgc ccaggagagc
    11101 cgcccgcgcg tcgcgcgcat ccagcaggct ccgccggagg ccctcgaggt ccaccttctc
    11161 cgcctagtcc ttcacctgcc tgaccgcgtc gatgatcgac ccgtcgcggt actcgatcag
    11221 cgccacgatg cgatcctcga actcgagcgg ccgcggcgtc cccgtcaggg cgcagatctc
    11281 cgcctgcaac tcgcggatgt ccttcagggg cagccttcgg cgcacgagct ccttcttcag
    11341 gtcggggtga cggtcggcca cggcgatgcc ccgctcggtc accaccacgt cgatcgtctc
    11401 gcccggggtc gtgatcgtcg tcacctcgtc gcgcacgatc ggcagccgcc cgcggatcgc
    11461 cggcgcgacg acgatcgaga gcttcgcccc cgccgccgtg tcgcagtggc cgccggtgtt
    11521 gtggagcagc aggccgttcg actcggtgtt gacgttgacg ttgaagttca cgtccacctc
    11581 ggtggccccg aggatgacgc agtcgagcat gttcaccacc gagccccggt tgaacgggtt
    11641 ggcgtagagg tcggcgctga tctcgcggtg catcaggttc ctgccgatcg actccaccgc
    11701 ccgcaggtcg aagcactgca cgtcgaacag cgtccggaag tacccctcct cgagcatgtc
    11761 cacgaagtag ccggtgatgc cgccgcagcc gaagcttccc ttgaccgacc cggccagcat
    11821 cttcgtcttc accgcgtcgg cgaccgccag cgaggtgcca cccgcgcccg tctggaacga
    11881 gaagccgtcg cgcagcaggc ccgccgcctc gatcacctcc gccgcgtagt gcgcgatcag
    11941 cagccccgcc ggatcgcggg tcacgcgcgt ggtggtcgac acgatcttct tcgggtcgcc
    12001 cagcgagtcg atcttcacca cgtagtccac gtcgctctgc gaaatgctga ccggcgacgc
    12061 ggggtacggg acgaggtggt cggtgacggc gatcaccttg cgcgcgtagc gcgcgtcggt
    12121 gaaggcgtag ccgaggctcc cgcaggccga cgggccgctc acaccgttga ggttccccgt
    12181 ctcgtcgcag cacggcgcgg cgatgaaggc gacgtccacc accacctcgt cggtgacgag
    12241 cgaccgcgcg cgccccccgt ggctgcggac gacgatcggg aagtcgagtt ctccccgcga
    12301 cgccagctcg ccgatcgggc cgttgacgcc ggtctcgcag cgcgtcacga cgcccttgcg
    12361 gatcgatggg acgatcgccg cgtgaacggg gtgcaccgag ctggaggcga tggcgatgtc
    12421 gcggaacccc atcgcgtcca gctcggcgac gaccaggttg aggagcgcgt ccccgttgcg
    12481 gaggtggtgg tgcgtggcga tggtcatgcc gtcgcgcagg cccgatcgct cgatcgccgc
    12541 ccgcagcgac gggagcacct tgctctcgcc cgggttgcgc cgcaccagcg gccgggtagc
    12601 gcggttcacc gccggcatgc gcgaccacgg gtcgcggtac ggctcgtagc accgcccgtt
    12661 ccagcgctcc ggtacctcgc gccccaggct attcctggcc atgcaccacc tcgtcggaca
    12721 gctcgtagtc gagcatcccg aacgcgacgg ccgtccggat ggtgcgcgcc gcccgcttga
    12781 ccaccggggc gtccaccatc ctgccccgca gggagatgac gccggtgccc atctcgcgcg
    12841 ccttcttgat cgcctccatg acctcgagcg cgtgctcgat ctcctcgccg gtcggccgga
    12901 agacctcgtg cacgaccgcg atctggcgcg ggttcacgag cgacttgccg gtgaagccga
    12961 gccgcttgac cagctcggtg tcggcccgca gcccctccat gtcgttcgcg tcggcgaaga
    13021 cactgtcgat cgcctgaagc ccggccgccc gcgccgccca gacgatttgc atgcgggccc
    13081 ccaggagctc atcgcccgtg cgcgtcctct ggatctccat cgacgccgtg tagtcctcgg
    13141 ccccgaatgc cagcgcgacg atccggtgcg acgcggttgc cgtcttcacg cagttcagga
    13201 ccccgacggc gctctcgatc gaaggaacga tggagaagaa cccgatgtcg aggccgagct
    13261 tctcctcgta ctcggtcagc agcgtgtcca gccgctcgac cacttcggga tcgtccgcct
    13321 tgggcagccg gatgccgtcg ggccgcgccg gcagcacctc gcgcaggtcg tcgagcgcgt
    13381 acttggtgtc gagaccgttg atccggacga agatctcccg gctgcgcttc gggaacgcct
    13441 gcaggaagct tcgggcgagg atccgggcgg agtccttctc ggcgagcggc acggcgtcct
    13501 cgaggtcgat catcaccccg tccgcgtcga agacggggat gttctgcagc atccccggca
    13561 tgttgccggg gacgtagagg agcgaacgcc gcaggcgctg ccgccggtcg atcatggtca
    13621 ctggtcctcg aagaacaggg tgtccacgcc accccgcatc gagtgctggc gctccacccc
    13681 ctcgacgatg tactcgccga ggatgatctc gcaggtggcg aagacctcgg cgtcgcgcag
    13741 gtggccgccg accgcctcgc ccgacgaccg ggcggcgaag acgtgaaggt gcgcgtccac
    13801 gcgcccctcc tcgttcggaa ccaggttgcc ctcgaggccc agcaggtcga gcggcccctc
    13861 cagccggtgg accggcatcc gcggctcggt gatcggcagc cgggcgcccg cctggatgtc
    13921 ggtgaactcg acgtcgcgga tcgaaccca
//
LOCUS       DAOPQX010000060        13793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5195_length_13793_cov_4.265978, whole genome shotgun sequence.
ACCESSION   DAOPQX010000060 DAOPQX010000000
VERSION     DAOPQX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13793
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5195_length_13793_cov_4.265978"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKK95_05070"
     CDS             complement(<1..309)
                     /locus_tag="PKK95_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17617.1"
                     /translation="MQIDLSLTPAALSGPIDRLWRLSGAKIRAIERNHPPGSDTLVYT
                     AAGRYTSRGWTEWTLGFRYGSALLQFDATGEKRFLEIGRRGTLEAMGAHLTDMGVHDH
                     G"
     gene            888..1457
                     /locus_tag="PKK95_05075"
     CDS             888..1457
                     /locus_tag="PKK95_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC17618.1"
                     /translation="MKVSPRLFLAAFVAAAIAAAAPAQAQAPAQAPASGNPISDGSRL
                     MFQVVSGFVTKSAEKAPEELYAFRPTPEVRTFGQLIGHAADAAYLICASAMGEKPPVE
                     GIEKTKTTKAELVKALTDSIAYCQKAYGGLTDATAGEQLPFFHGKMARVSILDFSTAH
                     LYEHYGNMVTYMRLKGIVPASSEKQPAGN"
     gene            complement(1477..3525)
                     /locus_tag="PKK95_05080"
     CDS             complement(1477..3525)
                     /locus_tag="PKK95_05080"
                     /inference="COORDINATES: protein motif:HMM:NF021534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HOC17619.1"
                     /translation="MKGAFEIREPDFDLSPLTGMTRKHYLDCARYVLGRAFRHVASPD
                     RPLSFPLVPGKTYPQPGDPPWRHRAHEFEAIERTFTLAGPLLHVDPAAAINNVSLRDY
                     YCRQVYNAFTPGHPGSIPWPEELPDATYQFTCEFGGLFKTLLLLPDVLWPWFTDEQKR
                     EMLPTISKWAHHRTTQNNWRIFNIVALSFLRKHGYEIDDDLLKSHLMWVASYHSGDGW
                     YLEQTYNYYTISLFVVYVTIWNRAFGDEHYPEIAAALEQTARRLMATYPRFFGRDGFV
                     NMWARSICYRTWISGGFPVSFMLKGEPPLDPGWARRLCSGSLLQFLTREDFFENDVPS
                     LGFYGHREFVLQSYSCPASPFIMFLPFIALALPEDSPFWTAREHDGPWEALGGRSVRT
                     VLERPGLVLVNHGSTGTSEIVPGKTYYEDRNYSKLAYNTHFPWEDHDPDGGTAMEYSF
                     RSLDPRDTRGEDVNFYLTGVAVPEEAARLARFNTSQAMLYNGVRGGVLYRQAIMRRPP
                     NDGIGYIVDLAEITIPGGVIRVDRSRLAFEHELTLGHYGLPHVDGRRAEVWRGAGRQV
                     ITASIPGRRLALVAYAGWDELRCTVHAGRNAEAEQSTVLYAHRRRTAKNPPMELMVAV
                     LLHRRDDEKWTEEELDPIRELRIEEVTPSGSMLGATLLLRSGETFVVDFAEIDGLRRC
                     "
     gene            3677..5662
                     /locus_tag="PKK95_05085"
     CDS             3677..5662
                     /locus_tag="PKK95_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOC17620.1"
                     /translation="MRPVSRFRIAWAVAAAAALGVALAAQQPSRPSADEVRAQYTKYE
                     YRVPMRDGARLFTSVYVPKTCTTGWPIMLNRTPYSVGPYGADNYRPALGPSEHFQENG
                     FIFVYQDVRGRYMSEGTWAEVRPHNPSKSSPKDVDEASDTYDTIEWLVRHVPCNNGRV
                     GMWGVSYPGFYVSAGMIDAHPALKAASPQAPVTDYYLGDDSYHNGAFMLAANFGFYGA
                     FQPRLGAPQPPGPRVPFDYGTPDGYEFYLNMGSLRAGAEKYGLSSNPHYMINLDHTTY
                     DDFWKARSIWRHFSKLTAAVLTVGGWFDAEDLQGPLRTYRQVAQASPSTSNRIVMGPW
                     THGGWSRGDGSGVGNVDFGQKTASYYRESIEFPFFARLLKDAKVSDLAAATMFETGTN
                     RWRTFDAWPPKGVTPRALFLRESGRLETEPPPAGDAFDEYVSDPMRPVPYVGYVQMGM
                     QRDYMTEDQRFAARRPDVLVYQTEPLDDDLTVLGPIKVTLHLSTTGTDSDFVVKVVDV
                     YPATYPQPEWTGPAPRPANYVRMGGYQQLVRGEPFRGKFRRSFEKPEPFVPGQPDLLE
                     FEMPDVAHTFRAGHRLMVQVQSSWFPLTDRNPQTFTDIPSAKPGEFRKATQRIFRSAA
                     RPSSITLLVEAPAAPLEVTNAGSAVSGGTRALSSRTR"
     gene            complement(5622..7154)
                     /locus_tag="PKK95_05090"
     CDS             complement(5622..7154)
                     /locus_tag="PKK95_05090"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOC17621.1"
                     /translation="MRVRTLVVLLVLASALVAAAPGQAPSTAGAPRTYCNPLPIPNYP
                     VGRLVRDLRAGDKTDGSFLWFVDRVEQYRELADPAALWHDGKWFLYPSVDMAWVSDDM
                     GATWQHHPLNVRDAGYAPTIVRQGSRFLLMASESAIYASDSPLGPFTELGRPKLPAGL
                     PVTTDPMLFADDDGRLFLYWGCTETAGIFGIELDPSDPTRPIGPAKKLIPFSPDAHPW
                     ERVGEHNQQRNAGWIEGAWMIRREGRYYLTYSAGGTEHRAYAMGAYVGTSPLGPFTPQ
                     AHNPIFRTTAGLVTGTAHGAIVRGPGDRWWVFYTIAAGAAHGFERRVGMDPVEFGPDG
                     QIRVPRATSTPQWLPGSGPGREGPADTGWVHLNGGVQTIASSSAPNVTGRLAVDNDLR
                     TWWMPVEGDPAPTLTSRLYAPASVHAVRIAWRDIGLDTNRGVRPGPYRYRVELETTAG
                     TWQTILDRTASNEDLLVDYREITPAVGRRARLVVTGWPKGITPGVAEFTVFGTTEPAS
                     RR"
     gene            complement(7217..8659)
                     /locus_tag="PKK95_05095"
     CDS             complement(7217..8659)
                     /locus_tag="PKK95_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOC17622.1"
                     /translation="MTPRYNLGYVWTISLVAAMGGLLFGYDWVVIGGAKPFFEKSFGL
                     TTAASIGWANSCALVGCLAGSLVTGALCDRFGRKRLLVASALLFAGSSVLTGWAGSFG
                     AFVAWRITGGTAIGMASTLSPMYIAEVAPPHLRGRLVAVNQLTIVIGILSAQVVNWII
                     AVLLPFPGIADVEAARAAWNVAYGWRWMFTAVAVPSLVFLAGALVVPESPRWLIVSGQ
                     SERARRVLARIGGEAYASAALADIARTVSHETGPGAISSRRAVAELRALLDPAVRGIV
                     LVGVALAVLQQWSGINSIFNYAEEIYRSAGYGIGDIMFNIVITGTINLVFTLIAIAVV
                     DRVGRRALMLAGCAAIGVSHLLLGAAYAAGLQGLPVLVFTLCSLGAYALSLAPVTWVL
                     IAEIFPNRVRGAAVSAAVSALWTACFILTFAFPLLQRTVGIAVTFWIYAAICFAGFVF
                     VRARVPETKGRSLEELEEDLGTAEQLTADR"
     gene            complement(8656..9915)
                     /locus_tag="PKK95_05100"
     CDS             complement(8656..9915)
                     /locus_tag="PKK95_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC17623.1"
                     /translation="MTSRERIAAACAHTEPDRLPVDFGGGFQTGIHVSVVYKLRQALG
                     LDAPGTPVKVVEVCQMLGEIAPDLAAALHADTVSLHGTGTMFGFPQAEFKEWQLADGT
                     PVLVPVDFNTAYEPNGDLLQWPANDRSVGPSARMLPGGPFFDPIERPQPIDPARLDPS
                     DNTEEFMLADEAELAHYARLADCLWSTTERALFCTFGGLTFGDIALVPGASLRQPRGI
                     RGIEEWYASTLTRPAYIRAVFERQAEIAVQNLERLHAAVGDRVAVIQTNGTDFGTQNG
                     PFCSPKMYRDLYMPYQQKVNGWIHAHTPWKTFMHCCGGIEPLLESIVEAGFDILNPVQ
                     CSARGMDPVDLKRRFGGRLVFWGGGVNTQRTLPFGTPAEVRDEVRRRIEIFAPGGGFV
                     FTAIHNVQADVPVENVLAMLEAVAEYR"
     gene            complement(9947..11548)
                     /locus_tag="PKK95_05105"
     CDS             complement(9947..11548)
                     /locus_tag="PKK95_05105"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOC17624.1"
                     /translation="MATRPRSLLLLPAVVFLGLAGVAAQATRSEGQAGGEPGILTPPP
                     PPTPRINGPSVYGVRPGRPFLYRIPATGDRPMRFRAEGLPAGLALDEATGIVRGTITD
                     LTPRSHELTLIASNARGRASRPFRIVVGDALALTPPMGWNDWYTFYEQPSDELMRKAA
                     DIMISSGMADHGYQYVNIDDAWMGKPGSNDPALTGPARDERGMINANRRFPDMKGMTD
                     YIHSLGLKAGLYTSPGPLTCADFHASHEHEAQDVARFVEWGFDFLKYDWCSYEKVAPK
                     GSAPDPERLQKPYRLMGALLKEQPRDIVFNLCQYGMGDVWTWGASVGGHSWRTTGDLG
                     LEKGQRLPGFYSIGFRNAEHSAFAGPGHWNDPDYILIGYVGNAHDINEAPKPTTLTPN
                     EQYSYMSMWALMAAPLFYSGDITRLDPFTLNVLNNAEVIAVDQDSLGIQARIVRKTDD
                     EFVLAKPLEDGSLAVGLFNLSEETRTIAVRMAELGVGPRRVRDLWRWKDVGVFADEYS
                     ASVGRHGVMLVRMEAGPRGGQRFRM"
     gene            11650..>13793
                     /locus_tag="PKK95_05110"
     CDS             11650..>13793
                     /locus_tag="PKK95_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOC17625.1"
                     /translation="MNSIVKASALAGCFVVFGVVVAGPRVGVCARQGAPCQALKEWTT
                     AEDHQQMMGQLGIKALRPGPSGNEKDPNHANYDETLANPFPDLPELLTLKNGRTVTDA
                     AGWAERRKEIAEDFEREVIGRIPPNVPRVSWSNAARQEATLAGIPVVGRQLVGRVDNS
                     ECPAIAVEIQLTLVTPADLKTRVPVMIMFRGGSLGQALGTAPPPAMPPRFTPPPGALG
                     YDPPATEQLLAAGWGFAFLNPSSIQADNGAGLTRGIIGLVNKGQPRKPDDWGSLRAWA
                     WGAARALDYLETDPAVDAKRVGIEGVSRYGKAALVTMAFEPRFAVVLVGSSGEGGAKL
                     HRRNFGEAVENLTGSGEYHWMAGNFLKYGASDAVFGSRNAGDIPVDAHQLIALCAPRP
                     TFISYGIPEMGDAKWLDQQGSFMAAVAAGPAFRLLGVKDLGTSDDYKTEKMPPVNVGL
                     LDGHLAWRQHDGGHTDAPNWKWFLPWANRLLGYQRPPRTWPADRPSPRWDANSLVAHQ
                     QLLAKAKAGRIDVYFLGDSITRRWGATDYPELLQNWKKNFFGWNAANFGWGADGTEHI
                     LWRLEHGELDGVNPKVIVVLAGTNNLGGETTGDGKVEEITRGLRAIVDACRAKAPGAT
                     VVLTAIFPRNDNLAVLPQIARVNANLAKMADGKTVRYLDVNDRLADKDGRLFPGMMGD
                     GLHPTVKGYQVWADALKPILTELLGPPAATDQA"
BASE COUNT         2220 a         4786 c         4768 g         2019 t
ORIGIN      
        1 gccgtggtcg tggacgccca tgtcggtcag gtgggcgccc atggcctcga gcgtcccgcg
       61 ccggccgatc tcgagaaacc gcttctcgcc ggtcgcatcg aactgcagca gggcggagcc
      121 gtagcggaag ccgagcgtcc actccgtcca gccgcgcgag gtgtaacggc cggccgccgt
      181 gtagacgagc gtgtcgctcc caggcggatg attccgctcg atcgctcgga tcttggcacc
      241 cgagaggcgc cagaggcggt ctatcgggcc ggaaagggcg gcgggcgtca gggagaggtc
      301 gatctgcata tcgcctgctg gccggggcaa gggcggccgg cgcaggcatt caaaccgatc
      361 cggcccggct cgtcaacggc tcagcgaatc ctcactccct tcacggccca cggaaaagcg
      421 ggtctcgatc gtgagaggct cctccgagac gtctccgagg ccgtggctgc gccacagcgc
      481 ggccagctcg ccgcgccggc aaagcggcat gtggcgctcg tcgcgcgcgt cccacggcgg
      541 gatcgagggc cactgccgcg tcccagaaga cgcgcagcat ctccaatccc tcgccgtagt
      601 cccacacggc cgcgaagacc accccgccgg gccgcgtcac acggaccatc tccttcatcg
      661 ccctggctga atcgggcacg aagttgagaa ccaggagcga gagcgtgcgg tcgaagtggg
      721 cgtctgccag ccgaagctgc tgggcggccg cgagaacaca cgcgacgcca gcgaaggtga
      781 gccaggcgga ggcgttcatg gctcacgatt acgcagcaag gccagtaccg aaacgcgcta
      841 ttcttggcag tgcatcccac aaaccggctc ttcagaagga gggtcccgtg aaagtcagcc
      901 cccggctctt cctcgcggcg ttcgtggcgg ccgccatcgc tgcggcagcg cccgcccagg
      961 cgcaggcgcc cgcgcaggcg cccgcgtcgg gcaacccgat cagcgacggc tcgcgcttga
     1021 tgttccaggt cgtcagcggc ttcgtcacca agtccgcgga gaaggcgccg gaggagctgt
     1081 acgccttcag gccgacgccc gaggtgcgga ccttcgggca gctgatcggc cacgcggcgg
     1141 atgccgccta cttgatctgc gcgtcggcca tgggcgagaa gccgcccgtg gaggggatcg
     1201 agaagacgaa gacgacgaag gccgagctcg tgaaggcgct caccgactcg atcgcctatt
     1261 gccagaaggc gtatgggggg ctgaccgacg ccaccgcggg ggagcagttg ccgttctttc
     1321 acgggaagat ggcgcgcgtg tcgatcctcg acttcagcac ggcgcacctg tacgagcact
     1381 acggcaacat ggtcacctac atgcgcctga agggtatcgt cccggcgtcg agcgagaagc
     1441 agccggcggg gaactagacc cggagctcat cggggctcag cagcgtcgca gtccgtcgat
     1501 ctcagcgaag tcgacgacga acgtctcgcc cgatctcagg aggagggtgg cgcccagcat
     1561 cgaaccggat ggcgtcacct cctcgatccg cagctcgcgg atcgggtcca gctcctcctc
     1621 ggtccatttc tcgtcgtccc gccggtggag caggacggcg accatcaact ccatcggcgg
     1681 gttcttcgcc gtgcggcggc ggtgcgcgta gaggacggtg ctctgctcgg cctcggcgtt
     1741 tcgccccgcg tgcaccgtgc agcgcagctc gtcccatccg gcgtaggcaa cgagggccag
     1801 ccgccggccg ggaatggagg ccgtgatcac ctgccggcca gcgccacgcc acacctcggc
     1861 tcgccggccg tccacgtgcg gcaggccgta gtgcccgagc gtcagctcgt gctcgaaggc
     1921 caggcgcgag cgatccacgc ggatcacgcc gcccgggatc gtgatctccg ccaggtccac
     1981 gatgtagccg atcccgtcgt tcggaggccg gcgcatgatc gcctggcggt agaggacgcc
     2041 gccgcgcacg ccgttgtaga gcatggcctg cgaggtgttg aagcgcgcca gccgcgccgc
     2101 ctcctccggc accgccaccc ccgtcagata gaagttgacg tcctcgccgc gcgtgtcgcg
     2161 cgggtcgagg ctgcggaagc tgtactccat cgcggttccg ccgtcgggat cgtggtcctc
     2221 ccacggaaag tgcgtgttgt aggccagctt cgagtagttg cggtcttcgt agtaggtctt
     2281 gcccggaacg atctccgatg tgcccgtgct cccgtggttc acgagcacga gccccgggcg
     2341 ctcgagcacg gtcctcaccg accggccgcc gagcgcctcc cacggcccgt cgtgctcgcg
     2401 cgccgtccag aagggcgagt cctcggggag cgccagcgcg atgaacggca ggaacatgat
     2461 gaacgggctc gccgggcagc tgtagctctg gaggacgaac tcgcggtggc cgtagaagcc
     2521 gagactcggc acgtcgttct cgaagaagtc ctctcgcgtg aggaactgga ggagcgaccc
     2581 tgagcagagc cggcgcgccc agcccgggtc gagcggcggc tcccccttca acatgaacga
     2641 cacggggaac ccacccgaga tccacgtccg gtagcagatg cttcgtgccc acatgttgac
     2701 gaagccgtcg cggccgaaga agcgcgggta ggtggccatc agccgtcgcg ccgtctgctc
     2761 gagcgcggcg gcgatctcgg ggtagtgctc gtcgccgaac gcgcggttcc agatggtcac
     2821 gtacacgacg aacaggctga tcgtgtagta gttgtaggtc tgctccaggt accagccgtc
     2881 acccgagtgg tacgacgcca cccacatcag gtggctcttc aacaggtcgt cgtctatctc
     2941 gtagccatgc ttgcggagga acgacagcgc gacgatgttg aagatgcgcc agttgttctg
     3001 ggtggtgcgg tggtgcgccc acttcgagat ggtggggagc atctcccgct tctgttcgtc
     3061 ggtgaaccac ggccacagca cgtcgggcag cagcagcagc gtcttgaaca gccccccgaa
     3121 ctcgcaggtg aactggtagg tggcgtcggg cagttcctcg ggccagggga tcgagcccgg
     3181 gtgccccggc gtgaacgcgt tgtagacctg ccggcagtag tagtcgcgca ggctgacgtt
     3241 gttgatcgcg gcggcgggat cgacgtgcag cagcggcccg gccagcgtga aggtccgctc
     3301 gatcgcctcg aactcgtgcg cccggtgacg ccagggcggg tcgcccggct gcggatacgt
     3361 cttgccgggc accagcggga acgacagggg ccggtctggc gacgcgacgt gccggaacgc
     3421 gcgcccgagc acgtaccgcg cacagtccag gtaatgcttc cgcgtcatac cggtgagcgg
     3481 gctcaggtcg aaatccggtt ctctgatctc gaacgcgccc ttcatcgcag ttccctcccg
     3541 gatgcaacgt gggcacgcta accccggggt gcgggacgtg tcaaacgagg ggaggcagcg
     3601 tgccggcatt tcttgacagc ggccggctgg acatggatag tccggacgtt cgcgtcgaac
     3661 gcggaggaac ccttcgatga ggcctgtctc acgcttcagg atcgcatggg ccgtcgccgc
     3721 ggcggccgcc ttgggcgtcg cgctcgcggc gcagcagccg tctcgcccgt ccgcagacga
     3781 ggtgcgtgcc cagtacacga aatacgaata cagggtgccc atgcgcgacg gcgcgcggct
     3841 cttcacgtcc gtgtacgttc cgaagacgtg cacgacgggc tggccgatca tgctcaaccg
     3901 cacgccctac agcgtcgggc cctacggcgc cgacaactat cgcccggcgc tcggcccctc
     3961 cgagcacttc caggagaacg gcttcatctt cgtctaccag gacgtgcggg gccgctacat
     4021 gtccgagggg acatgggccg aggtgcgccc gcacaacccg tcgaagtcct cgccgaagga
     4081 cgtggacgag gcgagcgaca cgtacgacac catcgagtgg ctcgtccgtc acgttccctg
     4141 caacaacggg cgggtcggca tgtggggcgt gtcgtacccg ggcttctacg tctcggccgg
     4201 catgatcgac gcccaccccg cgctcaaggc ggcctcgccg caggcgcccg tcaccgacta
     4261 ctacctgggc gacgactcct accacaacgg cgccttcatg cttgcggcca acttcggctt
     4321 ctacggcgcg ttccagcccc ggctcggtgc gccgcagccc ccgggcccgc gggtgccgtt
     4381 cgactacggc acacccgacg gctacgagtt ctacctgaac atgggatcgc tgcgagccgg
     4441 ggccgagaag tacggcctgt cgagcaaccc gcactacatg atcaacctcg atcacacgac
     4501 ctacgacgac ttctggaaag cccgttcgat ctggcgccac ttcagcaagc tgacggccgc
     4561 cgtgctcacg gtgggcggct ggttcgacgc ggaggacctg cagggcccgc tgcggaccta
     4621 ccggcaggtc gcgcaggcga gcccctcgac gagcaaccgg atcgtcatgg gtccgtggac
     4681 gcacgggggg tggtcgcggg gcgacggctc gggcgtcggc aacgtcgact tcggccagaa
     4741 gaccgcctcg tactaccggg agtcgatcga gttcccgttc ttcgcccggc tgctcaagga
     4801 cgccaaggtg agcgacctcg ccgcggcgac gatgttcgag accgggacga accggtggcg
     4861 caccttcgac gcgtggccgc cgaagggcgt cacgccgcgc gccttgttcc tgcgggaatc
     4921 gggccgcctc gagaccgagc cgccgcccgc gggcgacgcg ttcgacgagt acgtttccga
     4981 cccgatgcgg cccgtgccct acgtggggta cgtgcagatg ggcatgcagc gcgactacat
     5041 gaccgaggac cagcgcttcg ccgccaggcg ccccgacgtc ctcgtctacc agaccgagcc
     5101 gctcgacgac gacctgaccg tgctcggccc catcaaggtc acgctgcacc tctccacgac
     5161 aggcaccgac agcgatttcg tcgtcaaggt cgtggacgtg tacccggcaa cctacccgca
     5221 gcccgagtgg accggcccgg cgcctcgccc ggcgaactac gtgcgcatgg gcggctacca
     5281 gcagctcgtg cgcggcgagc ccttccgagg gaagttcagg aggagcttcg agaagcccga
     5341 gccgttcgtc ccgggccagc ccgatctgct cgagttcgag atgcctgacg tggcgcacac
     5401 gtttcgcgcg ggccaccggc tgatggtgca ggtgcagagc tcgtggttcc cgctgacgga
     5461 ccgcaacccg cagaccttca ccgacatccc gagcgcaaag ccgggggagt tcaggaaggc
     5521 gacccagcgg atcttccgtt cggcggcccg gccctcgtcg atcacgctgc tcgtcgaggc
     5581 gccggcggcg cctcttgaag tgacgaacgc cgggagcgcc gtcagcggcg ggacgcgggc
     5641 tctgtcgtcc cgaacacggt gaactcggct acgcccggcg tgatgccctt cggccagccg
     5701 gtgacgacca ggcgcgcccg ccggccgacg gcgggcgtaa tctcccggta gtccaccagc
     5761 agatcctcgt tgctggccgt gcggtcgagg atcgtctgcc aggtgcccgc ggtcgtctcc
     5821 agctcgacgc ggtaccggta gggaccgggt cgcacgccgc ggttcgtgtc caggccgatg
     5881 tcccgccagg caatccgcac ggcgtgcacg gaggccggcg cgtacagccg gctagtcagc
     5941 gtgggcgcgg ggtcgccttc aacaggcatc caccaggttc gcaggtcgtt gtccaccgcg
     6001 aggcgtcccg tgacgttcgg cgcgctcgac gaggcgatcg tctgcacgcc gccgttgagg
     6061 tgcacccacc cggtgtcggc cggcccttct ctgcccggcc cgctccccgg gagccactgc
     6121 ggcgtcgagg tcgcccgtgg cacgcggatt tgcccgtccg ggccgaactc caccggatcc
     6181 atgcccacgc gccgctcgaa gccatgcgcg gcgcccgccg cgatcgtgta gaacacccac
     6241 cagcggtcgc cgggaccccg gacgatcgcc ccgtgcgccg tcccggtgac gagtcccgcc
     6301 gtggtccgga agatcgggtt gtgcgcctgc ggcgtgaacg ggccgagcgg cgacgtgccg
     6361 acgtaggccc ccatcgcgta ggcgcggtgc tcggtcccgc ccgccgagta ggtcaggtag
     6421 tagcgcccct cgcgccggat catccacgcc ccctcgatcc agccggcatt gcgctgctgg
     6481 ttgtgctcgc cgacccgctc ccagggatgg gcgtcgggtg agaacgggat cagctttttc
     6541 gcgggcccga tcggccgggt ggggtcggag ggatcgagct cgatgccgaa gatccccgcc
     6601 gtctcggtgc agccccaata gaggaacagc cttccgtcat cgtctgcgaa cagcatcggg
     6661 tcggtggtga ccggcagccc ggcgggaagc ttcggccggc caagctcggt gaaggggccg
     6721 agcggagagt cagacgcgta gattgccgac tcgctggcca tcaggaggaa ccggctgccc
     6781 tgccgcacga tggtcggagc gtagccggcg tcgcggacgt tgagcgggtg atgctgccac
     6841 gtggcgccca tgtcgtcgct cacccatgcc atgtccaccg agggatacag gaaccacttg
     6901 ccgtcgtgcc agagggcggc ggggtcggcc agctcgcggt actgctcgac ccgatcgacg
     6961 aaccacagga acgacccgtc ggtcttgtcg ccggctcgca gatccctcac gaggcggccg
     7021 accgggtagt tggggattgg gagcggattg cagtaggtgc gcggcgcgcc ggcggtcgaa
     7081 ggcgcctggc ctggcgccgc cgcgaccagc gcggatgcaa ggacgaggag caccacgagg
     7141 gtacgaacgc gcatcgaaaa gtcctccact ggcactgtcg gggttgagcc ttgcgcaggc
     7201 ggcccgaagc cagctgtcac ctatccgctg tcagctgttc tgcggtcccc aagtcttcct
     7261 ccaactcctc gaggctgcgg cccttggtct cgggaaccct ggcgcggacg aagacgaagc
     7321 cggcgaagca gattgcggcg tagatccaga aggtgaccgc gatccccacc gtccgctgca
     7381 gcagcgggaa cgcgaaggtc aggatgaagc acgcggtcca aagcgccgag acggccgccg
     7441 acacggcagc gccgcgcaca cggttcggga agatctcggc gatgagcacc caggtcaccg
     7501 gcgccagcga cagcgcgtac gcgccgagcg agcacagcgt gaacaccagc accgggagcc
     7561 cctggagccc cgcggcgtag gcggctccga gcaggaggtg cgagacgccg atggcggcgc
     7621 acccggccag catcagggcg cggcggccca cccgatcgac caccgcgatt gcgatcagcg
     7681 tgaacaccag gttgatcgtg ccggtgatca cgatgttgaa catgatgtcg ccgatgccgt
     7741 acccggccga ccggtagatc tcctccgcgt agttgaagat cgagttgatg ccgctccact
     7801 gctgcagcac ggcgagggcc acgcccacca gcacgattcc ccgcacggca ggatcgagca
     7861 gggcccgcag ctcggcgacg gcccgccggc tggaaatggc gcctggcccc gtttcgtgcg
     7921 acacggtgcg cgcgatgtcg gccagggccg cggaggcgta agcctcgccg ccaatccgcg
     7981 ccagcacgcg ccgtgcgcgc tcgctctgcc ccgagacgat cagccaccgc gggctctcag
     8041 gcaccacgag cgccccggcg aggaagacga gcgaggggac agcgaccgcg gtgaacatcc
     8101 agcgccagcc gtacgccacg ttccaggccg cccgcgccgc ctcgacgtcc gcgatgccgg
     8161 ggaacggcag cagcacggcg atgatccagt tgacgacctg ggccgagagg atcccgatga
     8221 cgatggtgag ctggttgacg gcgaccagcc ggccccgcag gtgcggagga gccacctccg
     8281 cgatgtacat cggcgagagg gtcgaggcca tgccgattgc cgtgcccccc gtgatccgcc
     8341 aggcgacgaa ggccccgaac gagcccgccc acccggtcag gaccgacgag ccggcgaaga
     8401 gcagcgccga ggcgacgagc agccgcttgc gtccgaaccg atcgcacaac gcgccggtga
     8461 ccagcgatcc cgccaggcag ccgaccaggg cgcacgagtt cgcccacccg atggacgcgg
     8521 ccgtcgtcag gccgaagctc ttttcgaaga acggcttcgc tcccccgatc accacccagt
     8581 cgtagccgaa gagcaggccg cccatcgcgg cgaccagcga gatggtccac acgtagccga
     8641 ggttgtagcg cggcgtcatc ggtactccgc gacggcctcg agcatcgcga ggacgttctc
     8701 gacgggcacg tccgcctgca cgttgtggat cgcggtgaag acaaagccgc cgccgggcgc
     8761 aaagatctcg atgcgccggc gcacctcgtc gcgcacctcg gccggagtgc cgaagggcag
     8821 cgtccgctgc gtgttgacgc cgccgcccca gaacaccagc cggccgccga agcgccgctt
     8881 caggtctacc gggtccatcc cgcgggccga gcactgcacc gggttcagga tgtcgaagcc
     8941 ggcctcgacg atcgactcga gcagcggctc gatgccgccg cagcagtgca tgaacgtctt
     9001 ccacggcgtg tgcgcgtgga tccacccgtt caccttctgc tggtacggca tgtacaggtc
     9061 ccggtacatc tttgggctgc agaaggggcc gttctgcgtg ccgaagtcgg tgccgttcgt
     9121 ctggatgacc gcgacccggt cgccgaccgc cgcgtgcagc cgctcgaggt tctgcaccgc
     9181 gatctcggcc tgccgctcga acacggcgcg gatgtaggcg ggccgcgtca gggtgctggc
     9241 gtaccactcc tcgatgccgc gaatcccgcg cggctgcctg aggctggcgc cgggcaccag
     9301 cgcgatgtcg ccgaaggtga ggccgccgaa cgtgcagaag agcgcccgct cggtggtgct
     9361 ccacaggcag tcggccagcc gcgcgtagtg cgccagctcg gcctcgtccg ccagcatgaa
     9421 ctcctcggtg ttgtcggagg ggtcgagccg cgccgggtcg atcggctgcg gccgctcgat
     9481 cggatcgaag aaggggcccc ccggcagcat gcgcgcgctg ggacccacgc tgcggtcgtt
     9541 cgcgggccac tgcagcaggt cgccgttcgg ctcgtaggcc gtgttgaagt ccaccggcac
     9601 cagcaccggc gtcccgtcgg ccagctgcca ctccttgaac tcggcctgcg ggaacccgaa
     9661 catcgttccc gtgccgtgca ggctgaccgt gtccgcgtgc agcgcggcgg ccaggtcggg
     9721 cgcaatctcg cccagcatct ggcacacctc gaccaccttc acgggcgtgc cgggcgcgtc
     9781 gaggcccagc gcctggcgca gcttgtagac cacgctgacg tgaatgcccg tctggaaccc
     9841 gccgccgaaa tccaccggca gcctgtcggg ctcggtgtga gcgcaggccg cagcaatccg
     9901 ctcgcgcgat gtcatgcgat gccttctaca ttgttcattc ggccttctac attcggaacc
     9961 tctgcccgcc tcgcggcccg gcctccatcc gcacgagcat cacgccgtgc cgcccgacgc
    10021 tcgcgctgta ctcgtccgcg aacacgccga catccttcca ccgccacaga tcgcgcacgc
    10081 gacgcggacc cacgcccaac tccgccatcc tcactgcgat ggtccgcgtc tcctccgaca
    10141 ggttgaacag ccccaccgcc agcgacccgt cctccagcgg cttggccagc acgaactcgt
    10201 cgtccgtctt gcggacaatg cgggcctgaa tgccgaggga atcctgatcg acggcgatga
    10261 cctcggcgtt gttcaggacg ttcagcgtga acgggtcgag gcgggtgatg tcgccggagt
    10321 agaagagcgg cgccgccatc agggcccaca tcgacatgta ggagtactgc tcgttgggtg
    10381 tgagcgtggt gggcttcggc gcctcgttga tgtcgtgcgc gttgcccacg tagccgatga
    10441 ggatgtagtc ggggtcgttc cagtggcccg ggcctgcgaa cgccgagtgc tcggcgttcc
    10501 ggaacccgat cgagtagaag ccgggcagcc gctggccctt ctcgagaccg aggtcgccgg
    10561 tggttcgcca ggagtggccg ccgaccgacg cgccccacgt ccagacgtcg cccatcccgt
    10621 actgacacag gttgaacacg atgtcgcggg gttgctcctt cagcagcgcg cccatcaggc
    10681 ggtacggctt ctggagccgc tcggggtcgg gtgcgctccc cttcggcgca accttctcgt
    10741 acgagcacca gtcgtacttg aggaagtcga atccccactc gacgaaccgc gccacgtcct
    10801 gcgcctcgtg ctcgtgcgag gcatggaagt cggcgcacgt gagagggccg ggcgaggtgt
    10861 agaggccggc cttgagcccg agggagtgga tgtaatcggt catccccttc atgtcgggga
    10921 agcggcggtt cgcgttgatc atcccacgct cgtcgcgcgc cggccccgtg agcgccgggt
    10981 cgttcgatcc cggcttcccc atccacgcat cgtcgatgtt cacgtactgg tatccgtggt
    11041 cggccatgcc cgagctaatc atgatgtcgg cggccttccg catcagctcg tcgctcggct
    11101 gctcgtagaa ggtgtaccaa tcgttccagc ccatcggcgg cgtcagcgcc agcgcgtcgc
    11161 caacgacgat gcggaagggc cgactggccc ggccgcgtgc gttcgaggcg atgagcgtca
    11221 gctcgtggga gcgaggcgtc agatccgtga tggtgccgcg cacgattccg gttgcttcgt
    11281 ccagcgccag gcccgcgggc aggccctccg cgcgaaagcg catcgggcgg tcgcccgtgg
    11341 ccgggatgcg gtagaggaac gggcggccgg gccgcacccc gtagaccgac gggccgttga
    11401 tgcgaggcgt tggcggcggg ggcggcgtca ggatgccggg ttcgccgccc gcctggccct
    11461 cggaccgcgt ggcttgggcg gcgacgcccg caaggccgag gaagacgacg gcgggcagca
    11521 gcaggagcga acgaggtcgg gtcgccatgg cccgggattc tatccccgat tcgctacgaa
    11581 atggtggttg gcgggagggt cggtcagctg acccccgtcg gtggtaggat gccaccccgg
    11641 ggaggggcca tgaactccat cgtgaaggcg agcgcgctgg ccggttgctt cgtcgtcttc
    11701 ggtgtcgtgg tcgccggacc tcgcgtgggc gtctgcgccc ggcagggggc gccgtgccag
    11761 gcgctgaagg agtggaccac cgccgaggac caccagcaga tgatgggcca gctcggcatc
    11821 aaggcgctcc gcccggggcc gagcggcaac gagaaggacc ccaaccacgc gaactacgac
    11881 gagacgctcg ccaacccctt ccccgacttg cccgagctgc tgacgctgaa gaacggccgc
    11941 acggtgaccg acgcggccgg gtgggccgaa cggcgcaagg agatcgccga ggacttcgag
    12001 cgcgaggtga tcgggcggat cccgccgaac gtgcccaggg tcagctggtc gaatgccgcc
    12061 cgacaggaag caacgctcgc cggcatcccg gtggtcgggc gccagctggt gggccgcgtg
    12121 gacaactcgg agtgccctgc catcgcggtg gagatccagc tcacgctcgt cacgcccgcc
    12181 gacctgaaga cgagggtgcc ggtgatgatc atgttccgcg gcggcagcct cggccaggcg
    12241 ctcggaacgg cgccgcctcc agccatgccg ccccgcttca cgccgccgcc cggagcgctc
    12301 ggctacgatc cgcccgccac cgagcagctg ctcgcggccg gctgggggtt cgcgttcctc
    12361 aacccatcga gcatccaggc ggacaacggc gcgggactga cgcgcgggat catcggcctg
    12421 gtgaacaagg gccagccgcg gaagcctgac gactgggggt cgctgcgcgc ctgggcctgg
    12481 ggcgccgcgc gcgcgctcga ctacctcgag accgatcccg ccgtggacgc gaagcgagtc
    12541 ggcatcgagg gagtatcgcg ctacggcaag gccgcgctcg tcacgatggc gttcgagccg
    12601 cgcttcgcgg tggtgctggt cgggtcgtcg ggcgaggggg gcgcgaagct gcaccgccgc
    12661 aacttcggcg aggcggtcga gaacctcaca ggctcgggcg agtaccactg gatggccggc
    12721 aacttcctga agtacggcgc ctcggacgcg gtcttcggca gcaggaacgc cggtgacatc
    12781 ccggtggatg cccaccagct gatcgcgctc tgcgcgccgc ggcccacctt catcagctac
    12841 ggcatcccgg agatgggcga cgcgaagtgg ctcgaccagc agggcagctt catggcggcg
    12901 gtggcggccg ggcccgcctt ccggctgctc ggtgtgaagg acctcggcac gtccgacgac
    12961 tacaagaccg agaagatgcc cccggtcaac gtcggcctcc tcgacgggca cctggcgtgg
    13021 cggcagcacg acggcggcca caccgacgcg ccgaactgga agtggttcct cccctgggcg
    13081 aaccggctgc tggggtacca gcggccgccg aggacgtggc ccgccgaccg gccgtcgccg
    13141 cgctgggacg ccaactccct ggtggcgcat cagcagctgc tcgccaaggc gaaggcgggt
    13201 cgcatcgacg tctacttcct cggcgactcg atcacacggc gctggggcgc caccgactac
    13261 cccgagctgc ttcagaactg gaagaagaac ttcttcggct ggaacgccgc caacttcggc
    13321 tggggcgccg acggcaccga gcacatcctc tggcgactcg agcacggcga gctggacggc
    13381 gtgaacccga aggtgatcgt ggttctggcg ggcaccaaca atctgggggg cgagacaacc
    13441 ggcgacggaa aggtcgagga gatcacgcgc ggcctgcgtg ccatcgtgga cgcctgccgc
    13501 gcgaaggcgc cgggcgcaac cgtcgtcctg acggccatct tcccgcgcaa cgacaacctc
    13561 gccgtgctgc cccagatcgc ccgcgtcaac gcgaacctgg cgaagatggc cgacggcaag
    13621 accgtgcgct acctcgacgt caacgacagg ctggccgaca aggacgggcg cctcttccct
    13681 gggatgatgg gcgacggcct gcacccgacc gtcaagggtt accaggtgtg ggccgacgcg
    13741 ctgaagccga tcctcaccga gctgctcggc ccgccggcgg cgaccgacca ggc
//
LOCUS       DAOPQX010000061        13652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5257_length_13652_cov_4.231889, whole genome shotgun sequence.
ACCESSION   DAOPQX010000061 DAOPQX010000000
VERSION     DAOPQX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_13652_cov_4.231889"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKK95_05115"
     CDS             <1..330
                     /locus_tag="PKK95_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17626.1"
                     /translation="RLNYFRLSNRPVTDEQMRNPIGIIPVARRPRVILRYADARDLLV
                     SGLLDGGADIAQRPAVIDAPLGRGHVILFSNNPMWRGETQGSYALVFNAILNFDQLDA
                     GRKLDPR"
     gene            390..1409
                     /locus_tag="PKK95_05120"
     CDS             390..1409
                     /locus_tag="PKK95_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17627.1"
                     /translation="MRRIIPLFAAVLVLATLPWPPGPAIGAERESFMLAVLRRDGVVI
                     PFAHYNARTRGLLQRGGRWLNPWPEAGKDFEIPLQLSDVPDDWWPGRQPVTSWTAWPL
                     QGAKTEIESQAPVAVIAHCQLGLGIRTSYKPPEPPPPENVQPYPKDGLATSGPVTVER
                     PVLLEPSANEWKAASEAVATEVTYLEEKVVTSLTFGARWHHPASSRERATTPFTPETI
                     IRVTPRALYFEGVKTYGPPAGVVGRRGTQVPNRCNILTYAAGWVLFSSDGKPKVRASA
                     DVTDCNREGMVYSLPLGIIPQEDGVLSIVQVSGWGYERYEVLEVRGDEVKLLLAVPGG
                     WCRGT"
     gene            complement(1413..2513)
                     /locus_tag="PKK95_05125"
     CDS             complement(1413..2513)
                     /locus_tag="PKK95_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOC17628.1"
                     /translation="MGRFTGRRRPDPMMQTPHAPSRHKARATLLTLLVAAACVALFAF
                     TLRNVGLEEIGEGVRRLGWGGFATILALSGLRLVFRSLAWMRCVEGEPRLRFGEALDA
                     TLMGEALGNATPFANVISEPSKAAFVRGRVPFRSAFSAIVVENIFYTASVALMIGIGA
                     AAFLLAYSMPEYLRAVSYGALVGVAVVIGAACVLLKARVHPLSSAAGWLDARSLVPVW
                     VSGRIPRVRRFEDDITTFTARNGRSLAPLALYEAAFHLAGVAEVYVTLTLIVPGSVTL
                     LTALVVESTGRVINVLFKFVPLRLGVDEAGNALLALPLALLPASLVTLALVRKARILV
                     WTAVGIGLLFRRGLSVRRALDHAQAVAGEAKG"
     gene            2607..3596
                     /locus_tag="PKK95_05130"
     CDS             2607..3596
                     /locus_tag="PKK95_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17629.1"
                     /translation="MKRVRLAAALAVSALLLSTAAVSADVKMQQKTQVKFEGMLGRMM
                     NLFGGKAAKEGIVQTVSVQGDRKATLSGDSGEIVDLAEEKVYQLNLKDKSYTVKTFAE
                     IRREMEEAAQKAEKEARKSERDKDQPEMQVDFDVKESGQRKTINGFDCREVVTTITVH
                     EKGKPLEQGGGIVMTANSWIAPNVPGAKELADFNLRYAKQMMGAANAQQMMQAFAMYP
                     GMKEAMERFQSEQVNLEGTPIQTTVVLSAAGSPEQAQAAGREDSPRGGGGVMGGLMGR
                     FGRKKADPSKDAGAGATPGRTTIMTSTTETLSIATAVDAAALQVPAGLKLKNK"
     gene            3943..5202
                     /locus_tag="PKK95_05135"
     CDS             3943..5202
                     /locus_tag="PKK95_05135"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOC17630.1"
                     /translation="MVDIQRPASVARNKKIRRIVFAAVAIVAIGLITVALSRLKPAAP
                     TVERATVWVDTVKRGDITRQVRGLGTLVPEEIRWIPAVTDGRVERILIYPGTPVTPDS
                     ILLELSNPTQAQALLEAEQQLKGAEAQLLNLRSQLQNEVLAQEAAAATAAADYRAAKL
                     QAEADAQLASEGLVSSLTTKLSQVKAENLEGRDRTEQKRLQQMKDSVQARLATQQAEV
                     DRLRALLALRRSQTASLKVRPGMAGMLQQVEVQVGQQVTPGTNLARVADPTRLKAQLK
                     IAETQAKDIQIGQIATIDTRNGFIPGRVSRIDPAVQNGTVTVDVALTGELPRGARPDL
                     SVDGTIELEKLIDVLHVGRPSVGQEESAIQLFRLEADGVHASRAKVMVGRASVNTVEI
                     REGLQVGDQVILSDMSQFDAFDRVRLK"
     gene            5238..5948
                     /locus_tag="PKK95_05140"
     CDS             5238..5948
                     /locus_tag="PKK95_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC17631.1"
                     /translation="MDGNGNDPLIRLEGVTKVFYTDEVETHALSGIHMEIARGEYVSI
                     AGPSGCGKSTLLSILGLLDTPTAGNYTLNGKPVANLNMAERARIRNREIGFIFQSFNL
                     IGDLTVFENVELPLTYRGMKSAERKTRVTQALEKVGMAHRARHLPSQLSGGQQQRVAV
                     ARALVGDPLILLADEPTGNLDSRNGEAVMDLLRELHRAGATICMVTHDPRFTRHADRT
                     IHLFDGQVVEESMEATPA"
     gene            5945..6640
                     /locus_tag="PKK95_05145"
     CDS             5945..6640
                     /locus_tag="PKK95_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC17632.1"
                     /translation="MSALITLRGVEKFYQHGVSRTYVLRRVSLDIRDGEFVSIMGPSG
                     AGKSTLLHIIGMFDSAWAGEYEFLGEPVHALNQKARSALHKQYMGFVFQSYHLLDNLT
                     VYENLEIPLSYRNVKKSERASIVCDVLDRFQIVGKKDLYPNQLSGGQQQLVAVARAIV
                     ASPKVILADEPTGNLHSAQGREIMELFKRLNAEGTTIVQVTHSEVNASYSDRVVQLRD
                     GWVVQEDGTVGTA"
     gene            6648..8015
                     /locus_tag="PKK95_05150"
     CDS             6648..8015
                     /locus_tag="PKK95_05150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC17633.1"
                     /translation="MTRDLAPPRVLLADDQPDVLEALRLLLKGEGYDLQAVASPPALL
                     AALDAGDFDVVLMDLNYTRDTTSGREGLDLLPRIQALDPTLPIVVMTAWGSVDVAVEA
                     MRRGARDFIQKPWDNDRLLTILRTQAELARALRRGQRLEAENQALREQRPANLVAASP
                     AMRPVLELIARVGPSEANVLVTGENGTGKGVVATALHAASPRASRPMVTVNAGGLSEG
                     VFESELFGHVKGAFTDARTDRVGRFELADGGTLFLDEIANVALPQQVKLLRVLETGEY
                     ERVGSSKTRKVNVRVISATNADPFEEVAAGRFRQDLLFRLNTVEIRLPPLRERREDIP
                     LLAMHFLRQHAQRYRKRLTGFDAAALQALAEHAWPGNVRELDHAVERGVLMTQGPLVR
                     AADLSLKPSRDASSRLEDMSLEEVEAFLVRKAMARYDGNVSHAAEALGLSRSALYRRL
                     QRYGL"
     gene            8012..9361
                     /locus_tag="PKK95_05155"
     CDS             8012..9361
                     /locus_tag="PKK95_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC17634.1"
                     /translation="MSAARRPPRRIGHDQRVLLLALLAGAPAVIVSLALLWTGDYSPK
                     VRWTLTTLVLGCWCGFAAALRQRVVYPLQTLSNLLAGLREGEASVRARGAETHDALGD
                     VMLEANALAETIREQRLGALEATALLRKVMEEIDVAVFTFDGESRLRLLNRTAERLLG
                     RPAEQLLGRTAAELGLASCLEPATPPVVDMAFPGAIGRWEVRRGDFRQGGLPHRLLVL
                     SDISRTLRNEERQAWQRLMRVLGHEINNSLAPIKSIAGSLVSLLRREPLPPDWREDAG
                     RGLEVIAGRAEGLTRFTASYARLARLPQPRLAALDVEALVRRVAGLETRLPVAVFPGP
                     RLRIEGDADQLEQLLINVLRNAVDAALTTGGGVQAGWSASSGHVEIWVDDEGPGLAQT
                     GNLFVPFFTTKPGGSGIGLVLSRQIAEGHGGTITLTNRAGAPGCRALLRLPLTARSS"
     gene            9840..10904
                     /locus_tag="PKK95_05160"
     CDS             9840..10904
                     /locus_tag="PKK95_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOC17635.1"
                     /translation="MLHSLLAPLLVAIAVQAPSPPASPPSSPKADEIEMCLSCHGDPQ
                     MSVTLPSGEARSLAVDREVFARSVHGDKLGCVDCHTGMTEIPHPPRPFKTRREFTIAY
                     YEACKRCHFASYSKTLDSVHQAAVARGDVTAPVCVDCHGAHDITPPDRPRTRISATCA
                     RCHEGVSLTYAKSVHGRALTEDDNADVPTCADCHRSHDVAGPHAPGWRLQSPELCGSC
                     HASKSMMEKYGISTNVMQTYVADFHGMTASLQRKGAAEGGAVVALCIDCHGVHDIMRA
                     DEKGSAVMQANLLRTCQRCHPGATQNFSSAWLSHYEPTFDKAPMVYAVRIGYLVLIPF
                     MIGGLVLQILLHLWRVVVNR"
     gene            10901..11575
                     /locus_tag="PKK95_05165"
     CDS             10901..11575
                     /locus_tag="PKK95_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOC17636.1"
                     /translation="MSDYLVRFTLRQRIEHFSVMTLFTILVITGLPQKFFDSSWAEAI
                     ILGLGGIDRIRWVHRAAGILFTLFTAVHLTLMVGSVLTGTGSLSLVPTRRDFEDAIQQ
                     LRYYLGTADAPARFDRFDYRQKFEYWGLVFGAVIVISTGFVLLFPIAATRFLPGEIVP
                     ASKLAHGNEGLMAFLVVIIWHIYNAHLSPDVFPFDKTIFTGKISVERMRHEHPLEYER
                     IKAQEE"
     gene            11624..12538
                     /locus_tag="PKK95_05170"
     CDS             11624..12538
                     /locus_tag="PKK95_05170"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOC17637.1"
                     /translation="MLVVEALLRAWRVNDPAWRLRFRLVPVAVPILLVPSFFVLAPWR
                     TGARFASSSALFAGERWNLLRVGPAGAGDLVLVLAAGLGAWLFLRDALPPLVDRLRQG
                     PAAAAGAWLAPPALRVVVERQARRLALPPPAIRVVASRWPAFFAEGHAPGELVVSPPA
                     LEALSPPELDAAVAHELAHLRYGDPRWGYALIAVRAVNFFNPALHWAARAVVDEIERR
                     ADQAVVEAGHAPDALASAIVKLFDRSHPESAASGPLLERLARRVRRVGVERRRERLVT
                     APPSRLSHGWLRLALVACGLTTMLYFVV"
     gene            12572..13117
                     /locus_tag="PKK95_05175"
     CDS             12572..13117
                     /locus_tag="PKK95_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17638.1"
                     /translation="MREVGALLARYLLVMGGAAAALAAADRLPALLSGATHGARVYRT
                     LSAAESALGARLRLPSYYPDTLSWPPSRIEVAPGPPPVTAVHVTRRDGAREALVVCQS
                     IGAPADPPRWLLPPLEALQDVEVEVGLHQAGLVRRLTPGGLVVHDLSWTQGERRFTLR
                     YEGAVEDLLRMARSLGGDVTP"
     gene            13114..>13652
                     /locus_tag="PKK95_05180"
     CDS             13114..>13652
                     /locus_tag="PKK95_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17639.1"
                     /translation="MTALSLGSAWTRRGIQTRVMLLVGAGVLASLAILGGAAWYALTA
                     VADRLAGGRGLLARSVATHLDVVLEDELSILQGIPASPRFDLADEDPQPEGAAVRDAY
                     LRSRLARRVMLVARDLRVLVAEPSPSGPAPPAALVAEAFERGRPTVSPLAVEAGDRRL
                     YLVLPLRDWRGAVAVLAVAV"
BASE COUNT         2046 a         4918 c         4748 g         1940 t
ORIGIN      
        1 cggctgaatt acttcaggct ctccaaccgg ccggtcaccg acgagcagat gcgcaacccg
       61 atcggcatca tcccggttgc ccggcggccg cgcgtgatcc tgcggtacgc cgacgcccgc
      121 gacctcctgg tgtcgggcct gctcgacgga ggcgccgaca tcgcgcagcg gcccgcggtc
      181 atcgacgcgc cgctcggccg cggccacgtg atcctcttct cgaacaaccc gatgtggcgt
      241 ggggagacgc agggcagcta cgccctggtg ttcaacgcga tcctgaactt cgaccagctc
      301 gacgcgggga ggaagctgga cccgcgctga gggtgccgca ccgtcatacg tataatcggg
      361 tcagccactg ccccgaggaa aggcgagccg tgcgacggat cattcccctg tttgcagccg
      421 tgctcgttct ggccaccctg ccctggccgc cgggtcccgc catcggcgcc gagagggaat
      481 ccttcatgct cgccgtcctc cggcgcgacg gcgtcgtcat tcccttcgcc cactacaacg
      541 cccgtacgag aggccttctg cagcggggcg gacgctggtt gaacccgtgg ccggaggcag
      601 ggaaggattt cgagatcccg ctgcagctct cggacgttcc cgacgactgg tggcccggca
      661 ggcagccggt cacctcctgg actgcgtggc ccctgcaggg ggcgaagacc gagatcgaga
      721 gccaggcccc ggtggcggtc attgcgcact gccagctcgg cctcgggatc cggacctcgt
      781 acaagccgcc ggagccccct ccgcccgaga acgtgcagcc gtacccgaag gatgggctcg
      841 ccacctcggg cccggtcacc gtcgagcggc cggtgctgct cgagccctcg gcgaatgagt
      901 ggaaggcggc ctccgaagcg gtggcgaccg aggtcaccta cctcgaggag aaggtggtga
      961 caagtctcac gttcggcgcg cgctggcacc acccggcatc gagccgcgag cgcgccacca
     1021 cgccgttcac gcccgagacg atcatccgag tcacgccgcg cgccctctat ttcgagggcg
     1081 tgaagacgta cggccccccg gcgggggtgg tcggccgccg cggcacgcaa gttcccaacc
     1141 gctgcaacat cctcacgtac gccgccggct gggtgctgtt ctcgagcgac ggcaagccga
     1201 aggtgagggc gtcggccgac gtcaccgact gcaaccgcga ggggatggtc tacagcctgc
     1261 cgctcgggat catcccgcag gaggatggcg tcctgtcgat cgtccaggtg tcgggatggg
     1321 gctacgagcg ctacgaggtg ctcgaggtgc ggggcgacga ggtcaagttg ctgctggccg
     1381 tcccgggagg gtggtgccgg gggacgtaac cctcaccctt ttgcctcccc cgccaccgcc
     1441 tgcgcgtggt cgagcgcgcg gcgcaccgac aggccgcgcc ggaacagcag gccgatgccg
     1501 acggcggtcc acacgaggat gcgcgccttc ctcaccaggg ccagcgtgac cagcgaggcc
     1561 ggcagcagcg ccagcggcag cgcgagcagt gcgttgcccg cctcgtccac tcccagccgc
     1621 agcggcacga acttgaacag cacgttgatc acccggccgg tcgactccac taccagcgcg
     1681 gtcagcagcg tgacgctccc gggcacgatc aaggtgagcg tgacgtagac ctcggcgacg
     1741 cccgccaggt ggaaggccgc ctcgtacaac gcgagcggcg ccaggctgcg cccattcctc
     1801 gcggtgaagg tggtgatgtc gtcctcgaag cggcggacgc gcgggatccg gcccgacacc
     1861 cacaccggca ccagcgaccg ggcgtcgagc cacccggccg cagagctgag cgggtgcact
     1921 ctcgccttca acagcacgca ggcggctccg atgacgaccg ccacgccaac cagcgcgccg
     1981 tagctcaccg cgcggaggta ctccggcatg ctgtaggcga gcaggaacgc cgccgccccg
     2041 atgccgatca tcagtgcgac gctggcggtg tagaagatgt tctcgacgac gatggcggag
     2101 aaggcggagc gaaaggggac gcgaccgcgc acgaacgccg ccttcgacgg ctcgctgatg
     2161 acgttggcga acggcgtggc gttcccgagc gcctcgccca tcagcgtcgc gtcgagcgcc
     2221 tcgccgaacc gcagccgcgg ctccccttcg acgcaccgca tccacgccag gcttcggaac
     2281 accagccgca ggccggagag cgcgaggatc gtcgcgaagc cgccccaccc gagccgccgc
     2341 acaccctccc cgatctcctc gaggcccacg ttgcgcagcg tgaaggcgaa cagtgccacg
     2401 caggccgccg ccacgagcag ggtcagcagc gtcgcacgcg ccttgtgtct ggagggggcg
     2461 tgaggtgttt gcatcatcgg gtccggccgg cggcggccgg tgaacctccc cattctagcg
     2521 ggaacgctgc tatcatgtcg gccgtttccg cctcgtctgg cggtgctttc ccgtgcccct
     2581 gcgggtacgg ctgctcggag gaacccatga agcgcgtgcg actggcggcg gcgttggccg
     2641 tctctgccct gttgctctcg acggcggccg tttcggccga cgtcaagatg cagcagaaga
     2701 cccaggtcaa gttcgagggg atgctgggcc ggatgatgaa cctgttcggc ggcaaggccg
     2761 cgaaggaagg aatcgtccag acggtctcgg tgcagggcga ccgcaaggcg acgctctcgg
     2821 gcgactcggg cgagatcgtc gacctggccg aggagaaggt ctatcagctc aacctgaagg
     2881 acaagtcgta cacggtgaag acgttcgccg agatccgccg cgagatggaa gaggcggctc
     2941 aaaaggccga gaaggaggcc cgcaagtcgg agcgcgacaa ggaccagccc gagatgcagg
     3001 tggacttcga cgtcaaggaa tctggccagc gcaaaaccat caacggcttc gactgccgcg
     3061 aggtcgtgac caccatcacg gtccacgaga agggcaagcc tctcgagcag gggggcggca
     3121 tcgtgatgac cgccaactcg tggatcgccc cgaacgtccc gggcgcgaag gaactggccg
     3181 acttcaacct gcgctacgcg aagcagatga tgggcgccgc caacgcccag cagatgatgc
     3241 aggccttcgc gatgtacccc ggcatgaagg aagcgatgga aaggttccag agcgagcagg
     3301 tcaatctcga agggacgccg atccagacca ccgtggtgct ctcggcggcc ggctcgcccg
     3361 agcaggcgca ggccgccggg cgcgaggaca gcccgcgcgg gggtggtggc gtgatgggcg
     3421 gcctgatggg gcgcttcggc cgcaagaagg ccgaccccag caaggacgcc ggggccggcg
     3481 cgacgccggg ccggacgacg atcatgacga gcaccaccga gacgctcagc atcgcgaccg
     3541 cggtggacgc ggccgccctg caggtgccgg cggggctgaa actgaagaac aagtagcccg
     3601 ccacgccgga gccgcgcagg cggcgaaccg ggcttgcccg ccggagccgc gcaggcggcg
     3661 aaccgggctt gcccgccgga gccgccgcag cggcgaaccg ggcttgcccg ccggagccgc
     3721 cgcagcggcg aaggcgggtg ttcgttttcg ggacgaggcc gtgtcggcct cgcacaggcc
     3781 ggccccgggg gccggcctga cccctaccgc ataagtcctt tgtttcgttc tgattgggcg
     3841 tagctcaaga catggcacgc ctctcgcccc tcccccagcg acttgccgtg gagccaccgc
     3901 cgtgatcctt tcgccggctg ctctcgtcta agggaatcga gcatggttga tatccagcgt
     3961 cccgccagcg tcgcgcgaaa caagaagatc cgccgcatcg tcttcgccgc ggtggccatc
     4021 gtggcgatcg gcctgatcac ggtggccctg tcgcggctga agccggcggc cccgaccgtg
     4081 gagcgggcca ccgtctgggt tgacaccgtc aagcgtggcg acatcacgcg gcaggtgcgc
     4141 gggctgggta cgttggtccc ggaggagatc cgctggatcc cggccgtgac cgacggccgg
     4201 gtcgagcgca tcctgatcta tcctggcacc ccggtgacgc ccgacagcat cctgctcgag
     4261 ctcagcaacc cgacccaggc gcaggcgttg ctcgaggccg agcagcagct caagggagcc
     4321 gaggcgcagc tgctcaacct gcggagccag ctccagaacg aggtgctggc gcaggaggcg
     4381 gcggcggcca cggcggcggc cgactaccgc gccgcgaaac tgcaggccga ggccgacgcg
     4441 cagctggcgt cggagggcct cgtctcgtcg ctgacgacca agctgtcgca ggtgaaggcc
     4501 gagaacctcg aggggcgcga ccgcaccgag cagaagcggc tgcagcagat gaaggactcg
     4561 gtgcaggcgc ggctcgccac ccagcaggcc gaggtcgacc gcctccgcgc gctgctcgcg
     4621 ctgcgccgca gccagacggc ctcgctgaag gtgcggcccg ggatggccgg catgctgcag
     4681 caggtcgagg tccaggtggg ccagcaggtg acgccgggca ccaacctcgc gcgcgtcgcc
     4741 gacccgacgc gcctcaaggc gcagctgaag attgccgaga cacaggccaa ggacatccag
     4801 atcggccaga tcgccacgat cgacacccgc aacggtttca tccccggccg ggtgtcgcgc
     4861 atcgatccgg cggtgcaaaa cgggacggtc acggtggacg tggccttgac gggcgaactg
     4921 ccccgcgggg cgcggcccga cctcagcgtg gacggcacga tcgagctcga gaagctgatc
     4981 gacgtgctgc acgtggggcg gccgtcagtg ggccaggagg agagcgcgat ccagctgttc
     5041 cggctcgagg ccgacggggt gcacgccagc cgggccaagg tgatggtggg gcgcgcctcg
     5101 gtcaataccg tggagatccg ggaggggctg caggtgggcg accaggtcat cctctccgac
     5161 atgtcccagt tcgacgcgtt cgatcgggtg cggctgaagt agggcaacag gacaaccagg
     5221 gcatcgcggg aggcacgatg gacggcaacg ggaacgaccc cctgatcaga ctcgaaggcg
     5281 tgacgaaggt gttctacacc gacgaggtcg agacgcacgc gctctcgggc atccacatgg
     5341 agatcgcccg cggcgagtac gtgtcgattg ccggcccctc gggctgcggc aagtcgaccc
     5401 tgctgtccat cctcgggctg ctcgacacgc ccaccgccgg caactacacg ctgaacggca
     5461 agccggtggc caacctcaac atggccgagc gggcccgcat ccgcaaccgc gagatcgggt
     5521 tcatcttcca gagcttcaac ctgatcggcg acctgacggt cttcgagaac gtggagctgc
     5581 cgctcaccta ccgggggatg aagtcggccg agcgcaagac gcgcgtcacg caggcgctcg
     5641 agaaggtcgg catggcgcac cgggcgcgcc acctgcccag ccagctctcg ggcggccagc
     5701 agcagcgcgt ggccgtggcc cgcgcgctgg tcggcgaccc gctgatcctg ctggccgacg
     5761 agccgaccgg caacctcgac tcgcgcaacg gcgaggcggt catggacctg ctgcgcgagc
     5821 tgcaccgcgc cggcgccacc atctgcatgg tcacccacga cccgcggttc acccggcacg
     5881 cggaccggac cattcacctc ttcgacggcc aggtggtcga ggagagcatg gaggcgaccc
     5941 cggcgtgagc gcgctcatca ccctgcgggg cgtcgagaag ttctaccagc acggcgtcag
     6001 ccgcacctac gtcctccggc gggtgtcgct cgacatccgc gacggcgagt tcgtgtcgat
     6061 catggggccc tcgggggccg gcaagtcgac gctgctccac atcatcggca tgttcgacag
     6121 cgcctgggcc ggcgagtacg agttcctcgg cgagccggtc cacgccctga accagaaggc
     6181 gcgctccgcc ctgcacaagc agtacatggg gttcgtcttc cagagctacc acctgctcga
     6241 caacctcacg gtctacgaga acctcgagat cccgctctcc taccggaacg tgaagaagag
     6301 cgagcgcgcg tcgatcgtct gcgacgtgct cgaccggttc cagatcgtcg gcaagaagga
     6361 cctctacccg aaccagctct ccggagggca gcagcagctc gtggcggtcg cgcgcgcgat
     6421 cgtcgccagc cccaaggtga tcctcgccga cgagccgacc ggcaacctcc actcggccca
     6481 gggacgcgag atcatggagc tgttcaagcg cctcaacgcc gaggggacga cgatcgtcca
     6541 ggtgacgcac tcggaggtca acgcgtcgta cagcgaccgc gtggtgcagc ttcgcgacgg
     6601 ctgggtggtg caggaggacg ggacggtggg gacggcctga ccgtgccatg acccgcgacc
     6661 tcgcccctcc ccgcgtcctt ctcgccgacg accagcccga cgtcctcgag gcgctgcgcc
     6721 tgctgctcaa gggcgagggg tacgacctgc aggcggtcgc ctcccccccg gcgctgctgg
     6781 cggccctcga tgccggcgac ttcgacgtcg tgctgatgga cttgaactac acgcgcgaca
     6841 ccacctcggg ccgcgagggg ctcgacctgc tgccgcgcat ccaggcgctc gaccccacgc
     6901 tccccatcgt cgtgatgacc gcctgggggt cggtggacgt cgccgtggag gcgatgcgcc
     6961 gcggggcgcg cgacttcatc cagaagccgt gggacaacga ccggctgctg acgatccttc
     7021 gcacccaggc cgagctggcc cgtgcgctgc ggcgtggtca gcgcctcgag gccgagaacc
     7081 aggcccttcg cgagcagcgg cccgccaacc tggtggccgc ctcccccgcc atgcgcccgg
     7141 tgctggagct gattgcccgg gtcggcccgt cggaggccaa cgtgctggtg acgggcgaga
     7201 acggcacggg caagggggtg gtcgccaccg cgctccacgc cgcctcgccg cgcgcctcgc
     7261 gcccgatggt gacggtcaac gccggcggac tgtccgaggg ggtgttcgag agcgagctgt
     7321 tcggccacgt caagggggcc ttcaccgacg cccgcaccga ccgcgtcggc cggttcgagc
     7381 tggccgacgg cggcacgctc ttcctcgacg agatcgccaa cgtggccctt ccccagcagg
     7441 tcaagctgct gcgcgtgctc gagaccggcg agtacgagcg ggtcggctcg tcgaagacgc
     7501 gcaaggtgaa cgtccgcgtg atctcggcta ccaacgccga ccccttcgag gaggtggccg
     7561 ccggccgctt ccgccaggac ctgctcttcc gcctcaacac cgtcgagatc cggctcccgc
     7621 cgctgcgcga gcgccgcgag gacatcccgc tgctggcgat gcacttcctg cggcagcacg
     7681 cgcagcgcta ccggaagcgc ctcacgggat tcgacgccgc cgccctgcag gcgctcgccg
     7741 aacacgcctg gccgggcaac gtgcgtgagc tcgatcacgc cgtcgagcgc ggcgtcctga
     7801 tgacgcaggg ccccctggtc cgcgccgcgg acttgagcct gaagccctcg cgtgacgcct
     7861 cgtcacgcct cgaggacatg agcctcgagg aggtggaggc gttcctggtg cggaaggcga
     7921 tggcgcggta cgacggaaac gtcagccacg cggccgaagc cctcggcctg agccgcagcg
     7981 ccctctaccg gcgcctgcaa cgctacggac tgtgagcgca gcccgccgcc ccccacggcg
     8041 gatcggccac gaccagcgcg tgctgctgct cgccctgctc gcgggcgccc ctgcggtcat
     8101 cgtctctctc gcgctgctgt ggaccggcga ctactccccg aaggttcgct ggacgctgac
     8161 cacgctcgtc ctcggctgct ggtgcggctt cgccgccgcg ctccgccagc gggtggtcta
     8221 cccgctgcag accctctcga acctgctcgc gggcctgcgc gagggcgagg cctcggtgcg
     8281 cgcgcgcggc gccgagacac acgacgccct cggagacgtg atgctcgagg ccaacgcgct
     8341 cgccgagacg atccgcgagc agcgcctcgg ggcgctcgag gcgacggcgc tgctccgcaa
     8401 ggtgatggag gagatcgacg tcgcggtgtt caccttcgac ggcgagtcga gactgcgcct
     8461 gttgaaccgc accgccgagc gcctgctcgg gcggcccgcc gagcagctgc tcgggcgcac
     8521 ggccgcggag ctcggcctcg cctcgtgcct cgagccggca acgccccccg tggtggacat
     8581 ggcgtttccc ggcgcgatcg ggcgctggga ggtgcggcgc ggcgacttcc gccagggggg
     8641 gcttccccac cgcctcctgg tgctgagcga catcagccgc accctgcgca acgaggagcg
     8701 ccaggcctgg cagcggctca tgcgcgtgct ggggcacgag atcaacaact cgctggctcc
     8761 gatcaagtcg atcgccggca gcctggtcag cctcctgcgc cgcgagcccc tgccgcccga
     8821 ctggcgcgag gacgccggcc gcggcctcga ggtgatcgcc gggcgggccg aggggctcac
     8881 ccgcttcacc gcttcgtacg cgcgcctggc gcgcctcccg cagccgcgcc tcgcggcgct
     8941 cgacgtcgag gcgctcgtgc ggcgcgttgc cggcctcgaa acccgtcttc ccgtcgccgt
     9001 gttcccggga cctcgccttc gcatcgaggg ggacgccgac cagctcgagc agctgctgat
     9061 caacgtgctg cgaaacgcgg tggacgccgc gctgaccacc ggcggcggcg tccaggccgg
     9121 gtggtcggcc tcgtcgggcc acgtggagat ctgggtggac gacgagggac ccgggctcgc
     9181 gcagaccggc aacttgttcg tgccgttctt cacgaccaag cccggcgggt cgggcatcgg
     9241 cctcgtgctc agccgccaga tcgccgaggg ccacggcggc accatcacgc tgaccaaccg
     9301 ggccggcgcc cccggctgcc gcgcgctgct ccgcctgccg ctcacggcgc gatcgtcctg
     9361 acgcgctgaa gactctgcat tgaagcggac gtaacagacg cggcggcttg tccacgttca
     9421 ccacagaggc acagaggcac ggagacgcac gcagaggttt ctggtcatct tcaagatctc
     9481 cgcgccctct gcgccgaaac gtacgcgaca gacgtaccgg cttgtccacg ttcaacgcag
     9541 agacgcgcag acgcggaggc gtatgcacag atctcggatc attccagaaa tctctgcgtc
     9601 ctctgcgcct ctgcggtgaa cgtgatgacg tgcccggagg tgccggattt ccggcaagcg
     9661 cccggacccc gcaccgcact gttgaccggc gccctccaaa acagcgatat ttcccgacgt
     9721 cgccacgtgg cagccggctt gctgtagccg ggcagaatcg ccaccacatc ggctgccccc
     9781 gttcgacgcc ccgccccggc gggtgcggcg ccgccgggga ccgggagccg cgccgccgca
     9841 tgctgcactc actgctcgcc ccgctgctcg tcgcgatcgc cgtgcaggcg ccctcgccgc
     9901 cggcttctcc tccttcgtcg cccaaggccg acgagatcga gatgtgcctc tcgtgccacg
     9961 gcgatccgca gatgtcggtc accctgccga gcggcgaggc gcgaagcctt gccgtggacc
    10021 gcgaggtctt cgcgcgctcg gtgcacggcg acaagctggg gtgcgtggac tgccacacgg
    10081 ggatgaccga gatcccgcac ccgccgcgcc cgttcaagac caggcgcgag ttcaccatcg
    10141 cctactacga ggcgtgcaag cgctgccact tcgcgtccta cagcaagacg ctcgacagcg
    10201 tgcaccaggc cgccgtggca cgcggcgacg tcacggcccc ggtgtgcgtc gactgccacg
    10261 gcgcgcacga catcacgccg cccgaccggc cgcgcacccg catctcggcg acctgcgcgc
    10321 gctgtcacga gggggtctcg ctgacctacg cgaagagcgt gcacggccgg gcgctgaccg
    10381 aggacgacaa cgccgacgtg ccgacctgcg ccgactgcca ccggtcgcac gacgtcgcgg
    10441 gcccgcacgc gccggggtgg cggctccaga gccccgagct gtgcggcagc tgccacgcgt
    10501 cgaagtcgat gatggagaag tacggcatct cgaccaacgt gatgcagacc tacgtcgccg
    10561 acttccacgg catgaccgcc tcgctccaaa ggaagggcgc ggccgaggga ggcgcggtcg
    10621 tggcgctctg catcgactgc cacggcgtgc acgacatcat gagagccgac gagaaggggt
    10681 cggccgtgat gcaggccaac ctgctccgca cctgccagcg gtgccacccg ggcgccacgc
    10741 agaacttctc gtcggcgtgg ctgtcgcact acgagccgac cttcgacaag gccccgatgg
    10801 tctacgcggt gcggatcggc tacctggtgc tcatcccgtt catgatcggc ggcctcgtgc
    10861 tccagatcct gctgcacctc tggcgggtgg tggtgaaccg atgagcgact acctcgtccg
    10921 cttcaccctg cgacagcgaa tcgagcactt ctcggtgatg acgctcttca ccatcctggt
    10981 gatcaccggg ctgccgcaga agttcttcga ctcgtcgtgg gccgaggcga tcatcctggg
    11041 cctcgggggc atcgaccgca tccgctgggt gcaccgcgcg gccggcattc tcttcaccct
    11101 cttcacggcc gtgcacttga cgctgatggt gggaagcgtg ctcaccggca ccggctcgct
    11161 ctcgctggtg ccgacgcgcc gcgacttcga ggacgcgatc cagcagctgc gctactacct
    11221 ggggacggcc gacgcgccgg cgcggttcga ccggttcgac taccggcaga agttcgagta
    11281 ctggggcctg gtgttcgggg cggtcatcgt gatctcgacc ggcttcgtcc tgctcttccc
    11341 gattgcggcc acccggttcc tgccgggcga gatcgtgccg gcctccaagc tggcgcacgg
    11401 caacgagggg ctgatggcct tcctcgtcgt catcatctgg cacatctaca acgcccacct
    11461 gtcgcccgac gtcttcccgt tcgacaagac gatcttcacc gggaagatca gcgtcgagcg
    11521 catgcggcac gagcacccgc tcgagtacga gcggatcaag gcgcaggagg agtaactggc
    11581 ccgggcgctc gtcgcgcagt cgatcctcca cggcctggtg gccatgctcg tcgtcgaggc
    11641 gctgctgcgc gcgtggcgcg tgaacgaccc ggcgtggcgg ctgcgcttcc gcctcgtgcc
    11701 ggtggcggtg ccgatcctgc tggtcccgtc cttcttcgtc ctcgcgccct ggcgcaccgg
    11761 cgcgcggttc gcctcctcga gcgccctctt cgccggcgag cggtggaacc tgctgcgcgt
    11821 cggccctgct ggggccggcg acctcgtgct ggtgctggcc gcggggctgg gcgcctggct
    11881 gttcctgcgc gacgccctcc cgccgctcgt cgatcgcctg cgccagggac ccgcggctgc
    11941 agccggggcg tggctggcgc cgcccgcgct gcgggtggtg gtggaacgcc aggcccggcg
    12001 gctggcgctc ccgccgcccg ccatccgcgt ggtggccagc cggtggccgg ccttcttcgc
    12061 cgaggggcac gccccgggcg agctggtggt atcgcccccc gcgctcgagg cgctctcgcc
    12121 gcccgaactg gatgcggcgg tggcccacga gctggcccac cttcggtacg gcgacccaag
    12181 gtggggctac gcgctcatcg cggtgagggc cgtgaacttc ttcaacccgg cgctccactg
    12241 ggcggcaagg gccgtcgtgg acgagatcga gcggcgcgcc gaccaggcgg tcgtcgaggc
    12301 cggacacgcg cccgacgcgc tcgcctcggc gatcgtgaag ctgttcgacc gcagccaccc
    12361 cgagtccgca gcctcggggc cgctcctcga gcgcctcgcg cgccgcgtgc gccgggtggg
    12421 cgtcgaacgc cgccgggaac gcctcgtgac ggcaccacca agccggctgt cacacggctg
    12481 gctgcgcctg gcgctggtcg cctgcggcct gaccacgatg ctgtacttcg tcgtgtgacg
    12541 gaccacgaag gacacgaaga ctttggaggg catgcgggag gtgggagcgc tcctcgcgcg
    12601 gtacttgctc gtgatgggcg gcgcggccgc ggcgctggcg gccgccgatc gcctgccggc
    12661 gctgctgtcg ggggcgacgc acggcgcccg ggtgtaccgc accctctcgg cggcggagtc
    12721 ggcgctgggc gcccgcctgc ggctgccgtc ctactacccc gacacgctgt cgtggccgcc
    12781 ctcgaggatc gaggtggcac ccggtccgcc cccggtgacc gccgttcacg tcacccggcg
    12841 tgacggcgct cgggaggccc tcgtcgtctg ccagtcgatt ggcgcgccag ccgacccgcc
    12901 gcggtggctc ctgccaccgc tcgaggcgct gcaggatgtc gaggtcgagg tcggcctcca
    12961 ccaggcgggg ctcgtccgcc ggctgacgcc ggggggcctg gtggtgcacg atctctcgtg
    13021 gacgcaaggc gagcgccggt tcacgctccg ctacgagggc gccgtggagg accttctgcg
    13081 catggcacga tcccttggtg gtgacgtcac accatgacgg ccctctcgct tggcagcgcg
    13141 tggacgcggc gcggcatcca gacccgcgtg atgctgctcg tcggcgccgg tgtcctcgcc
    13201 tcgctcgcga tcctcggggg cgccgcctgg tacgcgctga ccgccgtcgc cgaccgcctc
    13261 gcgggcggtc gcggcctgct cgcccgctcg gttgccaccc acctggacgt cgtgctcgag
    13321 gacgagctgt cgatcctcca ggggattccg gcctcgccgc gcttcgatct cgccgacgag
    13381 gacccgcagc ccgagggtgc ggccgtccgc gacgcgtacc tgcgctcgcg cctggcgcgc
    13441 cgagtgatgc tcgtcgcgcg cgatctccgc gtgctcgtcg ccgagccttc gccctccggg
    13501 ccagccccgc ccgccgcgct ggtggccgag gcgttcgagc gcgggaggcc gaccgtctcg
    13561 ccgctcgccg tggaggcggg cgatcgccgc ctgtacctcg tgcttcccct ccgcgactgg
    13621 cgcggagccg tcgcggtgct ggccgttgcg gt
//
LOCUS       DAOPQX010000062        13625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5271_length_13625_cov_5.007664, whole genome shotgun sequence.
ACCESSION   DAOPQX010000062 DAOPQX010000000
VERSION     DAOPQX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13625
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5271_length_13625_cov_5.007664"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKK95_05185"
     CDS             complement(<1..869)
                     /locus_tag="PKK95_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17640.1"
                     /translation="MARTSAAGEEDPIRPAAGKDAGAALSRAGFLLGLAVALVGAWHL
                     AAWVAGGEEAWSGFSVITTKANAALCLFLLGTALALGTRGDTRRARLRHLLAAACALC
                     AAVVGGLTVYEYLAGVDLGIDQLIVPQPEGALGGVAPNRMGLPVALGALFGGPAVLAF
                     VRRPALAVAGQVLGLGVVLLGLLSTIGYAYGVEALYGIAGVTAIAWPAALSLLATGLG
                     LLFARPSDGLMAVVTADDAGGHLLRRLLPPTLAVPFLIGWLRLLGERGEWYETSAGTG
                     LLMHVRRGAYVMDA"
     gene            complement(969..2285)
                     /locus_tag="PKK95_05190"
     CDS             complement(969..2285)
                     /locus_tag="PKK95_05190"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOC17641.1"
                     /translation="MPFPLDDYAARTPGSRAWYERARAVLPAGVSYGIRDLAPYPFYV
                     DRAEGPRVWDVDGNEYIDYWCGHGALILGHAAPPAVEAACRQAARGSHFGFAHTLEVE
                     MAEMVQRLVPGAEMVRYTNSGTEANMYALALARAFTGRARVAKMEGGWHGGLESLNTA
                     VHAPFGEPEALGLNPHATADTEALPFNDLDAARRALARGDLAALFVEPMMGAAGCIAA
                     EPGYLEGLRELCTRHGTVLVFDEVITGFRVAPGGAQQHYGVTPDITTLGKILGGGFPI
                     GALCGRQEVFERLDHRRFGGRSDRAFQGGTFSGNPVSLAAGLATLRQLEGGEVHERLG
                     RLAARLRDGIAAAFAEHGVPVGVTGLGSAVGVHFRSGAPRNAAEASADDLVVARRYFD
                     YMLGCGIVFLLPTSPHLFVSAAHTEAEIDGFLAATREFAPEARRSA"
     gene            complement(2346..3284)
                     /locus_tag="PKK95_05195"
     CDS             complement(2346..3284)
                     /locus_tag="PKK95_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17642.1"
                     /translation="MSRIPESGPSWLALEQAAARSCGRPDLSNQKDDANICVLAKALV
                     FARTGDEGRRAEVTEALRAVAAGRSYGGRALALGRELAAYVISADLIDLAGRDPSLDA
                     SFRSAIAGLLTARTSGGPKNLVECHEERPNNWGTHCGASRAAVAAYLDDAAQLERVAT
                     VFRGWLGDRDAYAGFKYGDLSWQCDPASPVGVNPPGCARGGHSIDGVLPDDQRRGGGF
                     TWPPPRENYVYEALQGALVQAVILQRAGYDAFAWQDSALLRAFRWLHEQARFPPSGDD
                     GWQVPLVNAFYGTSFPEPRPSRPGKNMGFTDFTHAR"
     gene            complement(3695..4771)
                     /locus_tag="PKK95_05200"
     CDS             complement(3695..4771)
                     /locus_tag="PKK95_05200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC17643.1"
                     /translation="MQIRVMHPSELEPRHLDAWRGLQSDHPSLDRPFFSPEYTQILGE
                     VRPEVEVAVLERGGEPVGFFPFQRVSPLVGRAPGLRLCDFQGLVAPPDLEIDPIVLMR
                     ACGLQAWHFDHVLEEQATWRDWHVTRSGSPLVDLTKGYDAYLAERRAAEANWVSQVPR
                     KARKMAREVGELRFVYDCRDQDVLSQVLSWKSLQRERTMTDDMLQHDWVRQALDAMLR
                     ANGPTFSCVLSALFAGPHLVAGHLGLRSGRALHLWFPAFDLAYERYSPGLVMLTEMFR
                     LAADAGIEWVDLGKGDDRYKQSLASATVLVTEGSVDTRRLRRLVGRAWYAGRARYRGS
                     RLAVLLTGPKRRLRRLVKRVAATG"
     gene            complement(4859..5977)
                     /locus_tag="PKK95_05205"
     CDS             complement(4859..5977)
                     /locus_tag="PKK95_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HOC17644.1"
                     /translation="MRGGTTRGVFFHAADLPADPPSRDAALLAVIGGSDPRQVDGLGG
                     ADLLLSKVALVWPSERPGAEVECRFGSITPGSGRVKYGSNCGNLSSAVACFARQEGLV
                     AADAAQVTIHNPDSGTFMEARWRDWEPQLRSRMASTSGMPVSGELVELTFLSPEATAT
                     GHLLPTGRAIDCLKLPDGRPVQASIVDAGALYVFLDPGQVGLPEGATSDQLRGNARLA
                     ALCEDLRGQAAVLAGLADEAAEALARTPAVPKLSFVRPPADYRAEGSGVEVEASEIDL
                     VGRIVSSQSFHKAYAVTGAIATAAAAAVPGSVVAAAMGGEWTPGTRRVRIGHPSGIIE
                     CTLETCVSGSGIEIRRAGVLRTARRIMEGRCFFDEPAA"
     gene            complement(6019..7278)
                     /locus_tag="PKK95_05210"
     CDS             complement(6019..7278)
                     /locus_tag="PKK95_05210"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOC17645.1"
                     /translation="MILTGNAAPPRSPKSAGEIALALSRSLGRRRVPVYRFHPERSLI
                     DLESRYCTHVPCPNLYDEGPALVTALLSFAKAQPAKPVLFPAADGAVEFVAQNEEVLG
                     KAFALACARWECVQEVQNKQRLLEHASRIGVPIPDTCFPKSASELDAFASSLQYPAVV
                     KPLTSHHWKRPEVAAVVEGAKALVVNDAGELGRVYRRVAPLAPDMMVQEIIPGEIDRL
                     LTFLGYVGHDGTPLAGCVRKKLRQYPAGFGYCCLAETVRDPEIMELSIRLLTSLGYRG
                     IAGVEFKRDPRTGKAKLIEINARAVRTTSTAIGAGVDLPWIAYQDLANPDSRPSPVFD
                     YRVPVRWIHVRSEIRAAIPMIRRGELSFWKWIRIFRGPKAQVMWAWDDPKPGLLNVLL
                     PTFHRLSQKFVPRKGTPAALPLGAARQ"
     gene            7644..8492
                     /locus_tag="PKK95_05215"
     CDS             7644..8492
                     /locus_tag="PKK95_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC17646.1"
                     /translation="MKVTTEANWKHDVRHRFSESGSARRWSEMYGGETDSPEEHFFRR
                     RRDFTVQHLLAHCGPDARILDLGCGSGPVTAALRRAGRQVIALDYSLDMLRLAGERME
                     REGQRASSFLQGDSERLPFPSGTFDAVVCLGVISYVRDYRSVLREVRRILTPEGRLVL
                     SSRNEWNPRFSDPVRPVKEAVRWLRQPRGGSRKMVIGRFLSPFQVERRLREVGFAIDR
                     FTGMGFGPPRFNRREVLSASASIRLSDGLERLAARLGSTVPYRWLAYVNLWVCRNETR
                     SLSAEN"
     gene            complement(8525..9139)
                     /locus_tag="PKK95_05220"
     CDS             complement(8525..9139)
                     /locus_tag="PKK95_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HOC17647.1"
                     /translation="MLPVDFQVPAGAVLVAVGLLACFAGYRLFRAVLTLYGFVIGALF
                     ASTLVAPSQTMAMTVALVAGGVAGAAIMYFGYFVGVMLVGAGLGALAGQALWTQLFGH
                     PNAIVTLVLAVVGAVVAVKVQRYVIVIGTAFIGAQTAVAGAVALAGARLPRPRGAEPV
                     WVGHLGLPPVSEGWPFYAWIVLSVVGTLVQLGWGSKGAGRGRRG"
     gene            complement(9201..9797)
                     /gene="recR"
                     /locus_tag="PKK95_05225"
     CDS             complement(9201..9797)
                     /gene="recR"
                     /locus_tag="PKK95_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOC17648.1"
                     /translation="MPQPEPLTRLVEELQRLPGVGRKSAQRLAFHILRTPREDADRLC
                     QAVQDVKDKVTYCSVCNNITDIDPCAYCTDEGRDRRQICVVEEPQNVIGIEKTRDFRG
                     AYHVLMGAISPLQGIGPDELKIKGLLARVEQGGVEEVILATNPNVEGEATAIYLARLL
                     KPLGVRVTRIAMGVPVGSDLEYADEITVHKAMEGRREM"
     gene            complement(9799..10098)
                     /locus_tag="PKK95_05230"
     CDS             complement(9799..10098)
                     /locus_tag="PKK95_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOC17649.1"
                     /translation="MNIQQMMKQAQQMQERLQKQMAEMRVEATAGGVMVSVVVSGTKQ
                     LLSVKIDPEVVSKDDVEMLQDLIVAAVNDAHRKVDEDLQKQVGGLMGGLKIPGMF"
     gene            complement(10109..11884)
                     /gene="dnaX"
                     /locus_tag="PKK95_05235"
     CDS             complement(10109..11884)
                     /gene="dnaX"
                     /locus_tag="PKK95_05235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOC17650.1"
                     /translation="MAYQVLARKWRPQRFEDVVGQGGVTRTLQNAIRTGRIAQAYVFA
                     GPRGVGKTTSARILARGLNCMAGAGPTPDPCGRCEACVEIAGGRDMDVLEIDAATHTK
                     VETVREVIISGLSVPPVRDRYKIFIIDEVHQLSGHSFNALLKSIEEPPPHALFVMATT
                     ELQKIPETILSRSQVHEFRTIPESAIAAQLARIAEAEELSVAGDALALIARAADGSMR
                     DGLTAFDQVLAFAGTSVSVDDVSSVLGLVGRDLLLDILEAVADERAPAAFDLAARAVE
                     GGYDLRLLCRELSRLVRDLMVISVDRSRLADTSFVAAGERARVEALAGRFSREDLLRA
                     FDLVAKTESDLRLAEEPRYHLEMALLKWMHLRKLAPIGELVEQLRGPAGAAPRRGASG
                     PGGGGGRSAAPPAPGAARAISQRLQAPAVRRPAPSSGPSPSAPPAPPPVSSRPSAEDE
                     PGGDPGEAAAPLAGRGLKDALLAEIKRAKAGFYNMTAAQASTIEVEGDSVVFSFAGGQ
                     RFLQQQVEQARAWLEPMASTIAGRRMQVNAVLREAPAGQGSGGPSAPPDDLRATAASD
                     PDMKALLDILPLEIKSVEKLGKDGE"
     gene            complement(11927..12026)
                     /gene="ffs"
                     /locus_tag="PKK95_05240"
     ncRNA           complement(11927..12026)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKK95_05240"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(12110..13486)
                     /locus_tag="PKK95_05245"
     CDS             complement(12110..13486)
                     /locus_tag="PKK95_05245"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOC17651.1"
                     /translation="MSPLSPSPPPSLSTDLYELTMMAGYYVAGMRAPATFELYVRDLP
                     RNRSYLVAAGIEQALEYLEALRFTPEQIGYLRGLPMFEPVPAAFFDEHLPSVRFTGEM
                     WAVAEGTPVFAHEPILRVTAPIGEAQLVETALLATIGFQTSIASKAARVVHAAAGRAV
                     VEFGSRRAHGTEAACLAARAAYIAGCQGTSNVEAGFRFGIPVSGTMAHSWVMSFESEA
                     EAFGRFYELYGGRSTLLLDTYDSVAAAEAVGRSAMRPRSVRLDSGDLAVLSRQVRAIL
                     DAHAQTGTRIFATGDLDEHRIAALVAAGAPIDGFGVGTALSTSKDAPALGAVYKLVSF
                     ERGGRTIDAMKLSEGKRSYPGAKQVWRQKGGKTPARDVLSLAAEPGPPGARPLLERVM
                     EGGRRTAPPVPLASLREACRRALDELPPGLRRLDRVEPYPVTLSDPLRSLTERLAARA
                     QEPEGTDR"
BASE COUNT         2073 a         4836 c         4849 g         1867 t
ORIGIN      
        1 gcgtccatca cgtaggcgcc gcgccggacg tgcatcagca ggccggtgcc ggcgctcgtc
       61 tcataccact ctccccgctc gccaagcagt cgcagccacc cgatcaggaa gggaacggcg
      121 agcgtaggag gcaggaggcg ccgcagcagg tggccgcccg catcgtccgc cgtcacaacg
      181 gccatcagcc catcagaagg gcgcgcgaag agaagtccca ggcctgtcgc gaggagggaa
      241 agggccgcgg gccaggcgat ggccgtgacc ccggcaatcc cgtagagggc ttcgacgccg
      301 tacgcgtatc cgatcgtgga cagcaggccg agcagcacga ctcccaggcc gagaacctgt
      361 ccggcaaccg cgagcgcggg ccggcggacg aaggcgagaa cggcggggcc gccaaagagc
      421 gcgccgagcg ccacgggcag gcccatgcga ttcggggcga cgccccccag cgcgccctcg
      481 ggctgcggga cgatgagctg atcgatcccc aggtccacgc ccgccaggta ttcgtacacc
      541 gtcaggccgc cgaccaccgc ggcacacagg gcgcacgcgg cggcgagcag gtgacgcagg
      601 cgggcgcgcc gggtgtcgcc acgggtgccg agcgccagcg ccgtcccaag caggaacagg
      661 cagagggcgg cgttcgcctt ggtggtgatg accgagaatc cgctccaggc ctcctcccca
      721 ccggcgaccc acgccgccag gtgccatgca ccgacgagcg cgaccgcgag ccccaggagg
      781 aacccggcgc gtgaaagcgc tgcgccggcg tccttgccgg cggcgggccg aatgggatcc
      841 tcctcccccg ccgcactggt gcgagccatc tgtgatgcca cgcggcgact ccgcgacggg
      901 agagcaagcg acttctcgtt cgaatcggaa gagccagcaa cgaggctaac gccacggacg
      961 aggcggggtc aagctgagcg ccgcgcctcc ggcgcgaact ctcgggtcgc cgccaggaac
     1021 ccatcgatct ccgcctcggt gtgggcggcc gacacgaaga ggtgcgggga ggtcggcagc
     1081 aggaagacga tgccgcaccc cagcatgtag tcgaagtacc gcctggcaac caccaggtcg
     1141 tctgcagatg cttcggcggc gtttcggggc gcgcccgatc ggaagtgcac cccgacggcc
     1201 gacccgagac cggtgacgcc caccgggacg ccgtgctcgg cgaaggcggc ggcgatgccg
     1261 tcccgcagcc gggcagccag gcgaccgagc ctctcgtgca cctctccgcc ttcgagctgg
     1321 cgaagcgtcg cgagccccgc ggcgaggctc accgggtttc cgctgaaggt gccgccctgg
     1381 aacgcacggt cgctgcgccc tccgaaccgg cgatggtcca ggcgctcgaa cacctcctgc
     1441 cggccgcaga gcgccccaat cgggaagccg ccgccgagga tcttgccgag cgtggtgatg
     1501 tcgggcgtca cgccgtagtg ctgctgcgcg ccgcccgggg cgacgcggaa cccggtgatg
     1561 acctcgtcga acaccagcac cgtgccgtgg cgggtgcaca gctcccgcag cccctccagg
     1621 tagcccggct cggcggcgat gcagccggcc gcccccatca tcggctcgac gaacaaggcg
     1681 gccaggtcgc cgcgggccag cgccctgcgc gccgcgtcga ggtcgttgaa gggcagcgcc
     1741 tcggtgtcgg ccgtggcgtg cgggttcagg cccagcgcct cgggctcgcc gaacggcgcg
     1801 tgcaccgcgg tgttcagcga ctcgaggccc ccgtgccagc ctccctccat cttggcaacc
     1861 cgggcgcggc cggtgaacgc gcgcgcgagc gcgagggcgt acatgttggc ctcggtgccc
     1921 gagttggtgt agcgcaccat ctcggcgcca ggcaccaggc gctgcaccat ctcggccatc
     1981 tcgacctcga gggtgtgggc gaagccgaag tggctgccgc gcgcggcttg ccggcacgcc
     2041 gcctcgacgg cgggaggcgc cgcgtggccg aggatcagcg ccccgtgccc gcaccaatag
     2101 tcgatgtact cgttcccgtc cacgtcccac acccgagggc cctcggcgcg atcgacgtag
     2161 aacgggtacg gcgccaggtc gcggatgccg tacgacacgc ctgccggcag aactgccctg
     2221 gcgcgctcgt accaggcgcg ggagcccggc gtgcgcgccg cgtagtcgtc gagggggaag
     2281 ggcatgaggg gatgataacg cgacggcgtg acgggcaggc gccgggcgaa gccctgcccc
     2341 gcggctcacc gggcgtgggt gaagtcggtg aaccccatgt tcttgccggg ccgcgacggc
     2401 cgcggctcgg ggaaactcgt gccgtagaac gcgttcacca gcggcacctg ccacccatcg
     2461 tcgccgctcg gcgggaagcg cgcctgctcg tgcagccagc ggaacgcccg gaggagcgcc
     2521 gagtcctgcc acgcgaacgc gtcgtagccg gcccgctgca ggatgaccgc ctggacgagc
     2581 gctccctgca gcgcctcgta gacgtagttc tctctcgggg gcggccaggt gaagccgccg
     2641 ccccgccgct ggtcgtccgg cagcacgccg tcgatcgagt ggccccccct ggcgcagccc
     2701 ggcgggttca cgccgaccgg actcgccgga tcgcactgcc atgacaggtc gccgtacttg
     2761 aagccggcgt aggcgtcgcg gtcgccgagc catcccctga acaccgtcgc cacccgctcg
     2821 agctgcgccg cgtcatccag gtaggcggcc acggccgccc gcgacgcgcc acagtgggtc
     2881 ccccagttgt tcgggcgttc ttcgtgacac tcgacgaggt tctttggccc cccggaggtt
     2941 ctcgcggtga gcagcccggc aatcgcggac cggaacgagg cgtcgagcga cgggtctcgg
     3001 ccggcaaggt cgatgaggtc ggccgagatc acgtaggcgg ccagctcgcg gccgagcgcg
     3061 agcgcgcggc cgccgtagga acgcccggcg gcgacggcgc gcaaggcctc ggtcacctcg
     3121 gcgcgccgcc cctcgtcgcc ggtgcgcgcg aacaccagcg ccttcgccag cacgcagatg
     3181 ttggcgtcgt ctttctggtt gctgaggtcg ggccttccgc acgagcgcgc cgccgcctgt
     3241 tccagggcga gccaggaagg ccccgactcg gggatccgtg acaactcctc ccggctgatc
     3301 cagataccgc gacgggccga cgggttgggc gacggttgag gcggcgaggg ctgcggtgag
     3361 ggctgcggcg agggctgccc ggctgaaacg ggctcggcgc ggccttcggg gtcgctcggc
     3421 ccggccggtg ggttctcgct gcaggcgatc accgccgaca gaaccagcca ggcgaggagg
     3481 gcacggaacg ggggcgtgaa ggccatgggt cccagacccg ggttcggcta cgccctcgca
     3541 gcagcaatcg cggaccagcg agagccgcag caccctccgg gtatccagct attgtgaggg
     3601 gcggggcggg cggcggcatg ggaggaggat cccggttgcg ccgggaatgc cgttcccggg
     3661 acgccggccg ttccccggaa ggccagccgg gcgattaccc ggtggccgcg accctcttga
     3721 ccagccggcg cagccgccgc ttcgggccgg tgagcaggac ggccaggcgg ctgccgcggt
     3781 agcgggcccg gcccgcgtac caggcgcggc cgaccagccg ccgcagcctc cgggtgtcga
     3841 cggacccctc ggtcaccagg acggtggccg acgcgaggct ctgcttgtag cggtcgtcac
     3901 ccttgccgag gtcaacccac tcgatgcccg cgtcggcggc caagcggaac atctcggtga
     3961 gcatcaccag gcccggggag tacctctcgt aagcgaggtc gaaggccggg aaccagaggt
     4021 gcagggcgcg tcctgagcgc agcccaaggt gtcccgccac caggtggggc cccgcgaaga
     4081 gcgccgagag cacgcacgag aaggtggggc cgttcgcgcg cagcatcgcg tcgagggcct
     4141 ggcgcaccca gtcgtgctgc agcatgtcgt cggtcatcgt gcgctcgcgc tgcagcgact
     4201 tccacgacag cacctgcgac aggacgtcct ggtcgcggca gtcatagacg aaccgcaact
     4261 ccccaacctc acgcgccatc ttgcgcgcct tacgcggaac ctgcgaaacc cagttggctt
     4321 ccgcggcccg ccgctccgcc aggtaggcgt cgtacccctt ggtcaggtcg accaacggcg
     4381 acccgctgcg cgtgacgtgc cagtcgcgcc aggttgcctg ctcctcgagc acgtggtcga
     4441 agtgccacgc ctgcagcccg cacgcgcgca tgaggacgat gggatcgatt tccaggtcgg
     4501 gcggggcgac cagcccctgg aagtcgcaaa ggcgcagccc cggagcgcgg ccgaccagcg
     4561 gcgatacgcg ctggaacggg aagaagccaa ccggctcgcc gccccgctcc aggacggcca
     4621 cctcgacctc cggccgcacc tcgcccagga tctgcgtgta ctcaggtgag aagaacggcc
     4681 ggtcgagcga ggggtgatcg gactgcagcc ccctccaggc gtcgaggtgc cggggttcca
     4741 gttccgacgg gtgcatcacg cgaatttgca tctcgggtgt tccggggggc cgtaccgaaa
     4801 aagtgaaaaa cgtcacttat ctggtacggc cccattagag tgaacgcgcc gcggcacctc
     4861 aggccgccgg ctcgtcgaag aagcagcggc cctccatgat tcgccgcgcg gtgcgcaaca
     4921 cgccggcgcg ccggatctcg atcccgcttc cggagacgca ggtctcgagc gtgcactcga
     4981 tgatcccgct cgggtggccg attcgaacgc gccgggtgcc aggcgtccac tcgccgccca
     5041 tcgccgcggc caccaccgac ccgggcacgg ctgccgccgc tgcggtggcg atcgccccgg
     5101 tcacggcgta ggccttgtga aagctctggc tcgagacgat gcggccgacc aggtcgattt
     5161 cgctggcctc cacctcgacg ccggaaccct cggcccggta gtctgcgggc ggacgcacga
     5221 acgaaagctt gggcacggcc ggcgtgcgcg ccagcgcctc ggcagcttcg tctgccaaac
     5281 cggcgagcac cgccgcctgg ccgcgcagat cctcgcaaag cgccgccagc cgggcgttgc
     5341 cccgcagctg gtcgctggtg gccccctcgg gcaggccgac ctggccggga tccaggaaca
     5401 cgtagagcgc cccggcatcg acgatgctcg cctgcacggg ccggccgtcg gggagcttca
     5461 ggcagtcgat cgcgcggccg gtcggcagca ggtggccggt ggcggtcgcc tcgggcgaga
     5521 ggaacgtcag ctccaccagc tctccgctca cgggcatgcc gctcgtcgag gccatgcgcg
     5581 accgcagctg cggctcccag tcgcgccagc gcgcctccat gaacgttccc gagtcggggt
     5641 tgtggatcgt cacctgcgcc gcgtcggccg ccaccaggcc ctcctgcctg gcgaagcagg
     5701 caacggccga ggagaggttc ccgcagttgg agccgtattt cacgcgcccg ctgcccggcg
     5761 tgatgcttcc gaaccggcac tccacctccg cgcccggccg ctcggacggc cacacgagcg
     5821 cgaccttgct caacagcagg tccgcgccgc ccagcccgtc cacctggcgc gggtccgacc
     5881 cgccgatgac ggccagcagc gccgcgtcgc ggctgggggg atcagccgga aggtcggcgg
     5941 cgtggaagaa gacgccgcgc gtcgttccgc cgcgcatgac gacgcaccgt gccgagtgcc
     6001 tgcccgggcc gtgctcgttc actggcgtgc tgctcccagg ggcagcgccg cgggcgtgcc
     6061 cttgcggggg acgaatttct gcgacagccg atggaaggtc ggcagcagca cgttgaggag
     6121 gcccggcttc gggtcgtccc acgcccacat cacctgagct ttcggcccgc ggaagatccg
     6181 gatccacttc cagaagctga gctcgccgcg ccggatcatc gggatcgctg cgcggatctc
     6241 gcttcgcacg tggatccacc gcaccggcac gcggtaatcg aagaccggcg aggggcggga
     6301 atccgggttg gccagatcct ggtacgcgat ccagggcaaa tcgacgccgg cgccgatcgc
     6361 ggtgctcgtc gtgcgaaccg cgcgggcgtt gatctcgatc agcttcgcct tgccggtgcg
     6421 ggggtcgcgc ttgaactcca cacccgcgat gccgcggtac cccagcgagg tgagcagccg
     6481 gatcgacagt tccatgatct ccgggtcgcg caccgtttcg gccaggcagc agtagccgaa
     6541 gccggccggg tactgacgga gcttcttgcg cacgcagccg gcgagcggcg tcccgtcgtg
     6601 tccgacgtag cccaggaagg tgaggagccg gtcgatctca ccggggatga tctcctggac
     6661 catcatgtcg ggcgccagcg gcgccacgcg gcgatagacg cggcccagtt ctccggcatc
     6721 gttcaccacc agcgccttgg cgccctccac caccgcggcc acctcgggcc gcttccagtg
     6781 atgggaggtc agcggcttca ccaccgcggg atactgcagg gacgaggcga acgcgtccag
     6841 ctcgctggcg ctcttcggga agcaggtgtc ggggatgggc acgccgatgc gcgaggcgtg
     6901 ctcgagcagg cgctgcttgt tctggacctc ctgcacgcac tcccaccggg cgcacgccag
     6961 cgcgaacgcc ttgccgagca cttcctcgtt ctgcgcgacg aactcgaccg cgccgtcggc
     7021 agcgggaaac aggaccggct tcgctggctg cgccttcgcg aacgacagca gcgcggtcac
     7081 gagcgccgga ccctcgtcgt acaggttcgg acacggcacg tgggtgcagt agcgggactc
     7141 gaggtcgatg agcgagcgct ccgggtggaa ccggtacacc ggcacgcgcc ggcggccgag
     7201 gctccgcgac agagcgagcg cgatctcgcc ggccgacttc ggcgagcgcg ggggcgccgc
     7261 gtttccagtc aggatcaccg ccgggggcag aggcataaga gagggcttgg gactcaaggc
     7321 ttacgactca aggggctcag gttcagaaca cgcacgcgaa ccgctggcct gcactcaata
     7381 tccatgccct ccggatattt cgccgcttac gccacgagcg tcgcccacca acacgggtgg
     7441 acgccgcgca caccgcacga cttctggaca gctacgtgaa aggcttttgg acagcatttg
     7501 tcctagtttt cctaggatct ccgcttcgcc acgctcctac aacgcgagtt cgcccgcgcc
     7561 cgagcgtctc gcagggatgg caagcgcctt gcggaagagc gcatcccagc catggcttcg
     7621 ttgcggcagg aggagcgatc caggtgaagg tgacgaccga agcgaactgg aaacacgacg
     7681 tccgccaccg cttctcggag agcgggagcg cccggcgctg gagcgagatg tacggcggcg
     7741 agaccgacag cccggaagag cacttcttca ggcggcggcg cgacttcacg gtccagcacc
     7801 tgctggcgca ctgcggcccg gacgcgcgaa tcctcgatct cgggtgcgga tccgggcccg
     7861 tgaccgctgc cctgcgccgg gccgggcggc aggtcatcgc gctcgactac tcgctcgaca
     7921 tgctgcggct ggcgggtgaa cggatggagc gcgaggggca gcgggcgtcg agctttctcc
     7981 agggagacag cgagcggctg ccgttcccga gcggcacgtt cgacgccgtg gtgtgcctgg
     8041 gggtgatcag ttacgtgcgc gactaccgtt cggttctgcg cgaggtgcgg cgcattctca
     8101 cgccggaggg ccgcctcgtg ctcagctcgc gcaacgagtg gaacccgcgg ttctccgacc
     8161 cggtgcggcc ggtgaaggaa gccgtgcggt ggcttcgcca gccgcggggc ggttcgcgga
     8221 agatggtgat cggccgcttc ctgtcgccct tccaggtcga gcggcggctg cgcgaggttg
     8281 gctttgcgat cgatcgcttc acgggaatgg ggtttggccc gccgcgcttc aaccggcgcg
     8341 aggtcctgag cgcgtccgca tcgatccgcc tgtcagacgg gctggaacgc ctggccgccc
     8401 ggctcggatc gacggtcccg taccgctggc tcgcgtacgt gaacctctgg gtctgccgca
     8461 acgagactcg aagcctgagc gctgagaact gaggccgtga acgttcaaaa ctgaggacgg
     8521 acagctaccc tcttctcccc cgcccggccc ccttcgaccc ccatcccagc tgcacgagcg
     8581 tgccgaccac gctcaggacg atccacgcgt agaacggcca gccctcgctc accggcggga
     8641 ggcccaggtg tccaacccac acgggctcgg cgccgcgcgg ccgcggcagg cgcgcgccgg
     8701 cgagggcgac ggcgccggcc accgcggtct gcgcgccgat gaaggcggtg ccgatgacga
     8761 tcacgtaccg ctgcaccttc acggccacca ccgcgcccac cacagccagc accagcgtga
     8821 cgatcgcgtt ggggtggccg aaaagctgcg tccagagcgc ctgcccggcg agggcgccga
     8881 gcccagcgcc gaccagcatc accccgacga agtaaccgaa gtacatgatg gcggcgccgg
     8941 caacgccgcc ggcgacgagc gccacggtca tcgccatcgt ctggctcggg gcgaccagcg
     9001 tgctggcgaa cagcgcgccg atcacgaagc cgtagagggt aaggaccgcg cggaacagcc
     9061 ggtagccggc gaagcacgcg agcaggccga ccgccacgag gacggcgccg gcgggaacct
     9121 ggaagtcgac gggaagcatg gaaacggagg atagcgcagg cggggacctt tctaccttct
     9181 acattctgct ttctgcgatt tcacatctcc ctccgcccct ccattgcctt gtgcacggtg
     9241 atctcgtcgg cgtactcgag gtcgctgccg accgggacgc ccatggcgat gcgggtgacg
     9301 cgcacgccca gcggcttgag caggcgcgcc aggtagatgg cggtggcctc gccctcgacg
     9361 ttcgggttgg tggcgaggat cacctcctcg acgccgccct gctcgacccg cgcgagcagc
     9421 cccttgatct tcagctcgtc ggggccgatg ccctggagcg gcgagatggc gcccatcagc
     9481 acgtggtagg cgccgcggaa gtcgcgcgtc ttctcgatcc cgatgacgtt ctgcggctcc
     9541 tcgacgacgc agatctgccg gcggtcgcgc ccctcgtcgg tgcagtaggc gcagggatcg
     9601 atgtcggtga tgttgttgca caccgagcag taggtcacct tgtccttcac gtcctgcacg
     9661 gcctggcaga ggcggtcggc gtcctcgcgc ggcgtccgga ggatgtgaaa ggcgaggcgc
     9721 tgggcgctct tccggcccac gccgggaagg cgctgcagct cctcgacgag acgggtcagc
     9781 ggttcgggct gcggcacgct agaacatccc ggggatcttg agtccgccca tcaggccgcc
     9841 cacctgcttc tgcaggtcct cgtccacctt gcggtgggcg tcgttgacgg ctgccacgat
     9901 caggtcctgc agcatttcga cgtcgtcctt cgagacgacc tcggggtcga tcttgaccga
     9961 gagcagctgc ttggtgcccg agacgaccac cgagaccatg acgccgccgg cggtggcctc
    10021 gacgcgcatc tcggccatct gcttctgcag gcgctcctgc atctgctgcg cctgcttcat
    10081 catctgttgg atgttcatgg accagccctc actcaccgtc cttgccaagc ttctcgacgc
    10141 tcttgatctc gagcggcagg atgtcgagca gcgccttcat gtcggggtcc gaggcggcgg
    10201 tggcgcgcag gtcgtcgggc ggcgccgacg gcccaccgct cccctggccg gccggcgctt
    10261 cgcgcagcac cgcgttcacc tgcatcctcc gccccgcgat cgtcgaggcc atcggctcca
    10321 gccaggcgcg cgcctgctcg acctgctgct gcaggaaccg ctgccccccg gcgaaggaga
    10381 acacgacgct gtcgccctcg acctcgatgg tggaggcctg cgcagccgtc atgttgtaga
    10441 aacccgcctt cgcccgcttg atctcggcca gcagggcgtc cttcagaccg cggccggcga
    10501 gcggggcggc cgcctcgccg ggatcgccgc cgggctcgtc ttcggcggac ggccgcgagg
    10561 agacaggcgg gggagccggg ggcgcggagg gggaagggcc ggacgacggc gcggggcgcc
    10621 ggacagccgg cgcctggagg cgctgtgaga tcgcccgtgc ggcgccagga gccggcggcg
    10681 cggccgaacg gccgccgccc ccgcccgggc ccgaagcccc gcggcgaggc gcggcgccgg
    10741 cgggtccgcg caactgctcg accagctcgc cgatgggcgc cagcttgcgc aggtgcatcc
    10801 acttgagcag cgccatctcg aggtgatacc gcggctcctc ggccaggcgc agatcgctct
    10861 cggtcttggc gaccaggtcg aaggcgcgca gcaggtcctc gcgcgagaaa cgcccggcca
    10921 gcgcctcgac gcgcgcacgc tcgcccgccg ccacgaacga ggtgtcggca aggcgcgagc
    10981 ggtccaccga gatcaccatc aggtcgcgca ccaggcgcga cagctcgcgg cagagcaggc
    11041 ggaggtcgta gccgccctcg acggcccggg ccgcaaggtc gaaggccgcc ggcgcgcgct
    11101 cgtcggccac ggcctcgagg atgtcgagca gcaggtcgcg cccgaccagc ccgagcaccg
    11161 aggagacgtc gtccaccgac acgctcgtgc cggcgaaggc gagcacctgg tcgaaggcgg
    11221 tgaggccgtc ccgcatgctg ccgtcggcgg cgcgggcgat gagggccaaa gcatcgccgg
    11281 ccaccgacag ctcctctgcc tcggcaatcc tggcgagctg ggcggcgatg gccgactcgg
    11341 ggatcgtgcg gaactcgtga acctgcgatc gcgagaggat ggtctcagga atcttctgca
    11401 gctcggtggt ggccatcacg aagagggcgt ggggcggcgg ctcttcgatt gacttcagca
    11461 gcgcgttgaa cgagtggcct gagagctgat ggacctcgtc gatgatgaag atcttgtagc
    11521 ggtcgcgaac cggcggcacc gacaggcccg agatgatcac ctcgcgaacg gtctcgacct
    11581 tggtgtgggt cgccgcgtcg atctcgagca cgtccatatc ccgccccccg gcaatctcca
    11641 cgcaggcctc gcagcggccg caggggtcgg gggtcggacc cgcgcccgcc atgcagttga
    11701 ggccgcgcgc gaggatgcgg gccgaggtcg tcttaccgac gccgcgcggg ccggcgaaca
    11761 cgtacgcctg ggcgatacgg ccggtgcgga tcgcgttctg gagcgtgcgg gtgacaccgc
    11821 cctgccccac cacgtcttcg aatcgctggg gccgccactt gcgggcgagc acctggtagg
    11881 ccatgggaat ccaagtgtag acaggaagag cgagaccggc cccaccgcga cccggatggc
    11941 cctgcggcac atgacagtca ctgctgagcg ctgctgcctt ccggccctga cgcggttcgc
    12001 aggctggcat tgcacaggat ccgggccgcg gtgcggccgg ccccgctcgg acccttaagt
    12061 gtacttcgcc agcaccccag ggtccagtgc ggtccgcgac ctgcggggcc taccgatcgg
    12121 tgccctcggg ctcttgtgcc cgcgcggcca ggcgttcggt caacgaccgc agcggatcgg
    12181 acagggtgac cgggtagggc tcgacccggt cgagccggcg gaggcccggc ggtaactcgt
    12241 cgagcgcccg ccggcaggcc tcgcgcagcg aggcgagcgg gacgggtggc gcagtcctcc
    12301 ggcccccttc catcacgcgc tcgagcagcg gcctcgcccc cggcggcccg ggctcggcgg
    12361 cgagcgacag cacgtcgcgc gccggcgtct tccccccttt ctgccgccac acctgcttcg
    12421 ccccgggata cgagcgcttg ccctcgctga gcttcatcgc gtcgatcgtc cgcccgccgc
    12481 gctcgaagga cacgagcttg tacaccgcgc cgagcgccgg cgcatccttc gaggtgctga
    12541 gggccgtgcc gacgccgaag ccgtcgatcg gggccccggc tgcgaccagc gccgcgatcc
    12601 ggtgctcgtc gaggtcgccc gtcgcgaaga tgcgcgtacc cgtctgcgcg tgcgcgtcga
    12661 ggatcgcgcg cacctgccgg ctgagcacgg ccaggtcgcc gctgtcgagc cggaccgacc
    12721 ggggccgcat cgccgagcgg cccaccgcct cggcggccgc caccgagtcg tacgtgtcga
    12781 gcagcagcgt cgaacgcccg ccgtacagct cgtagaagcg cccgaaggcc tcggcctcgc
    12841 tctcgaagga catcacccac gagtgcgcca tggtccccga gaccgggatg ccgaaccgga
    12901 accccgcctc gacgttcgag gtcccctggc accccgcaat gtaagctgcc cgcgccgcca
    12961 ggcacgcggc ctcggtgccg tgcgctcgcc ggctgccgaa ctcgaccacg gcccgaccgg
    13021 cggccgcgtg gaccacccgc gccgccttgc tggcgatgga cgtctgaaag ccaatcgtcg
    13081 ccagcagggc cgtttcgacc agctgcgcct cgccgatcgg ggcggtcacc cggaggatcg
    13141 gttcgtgcgc gaacacgggc gttccctcgg ccacggccca catttcgccg gtgaagcgca
    13201 ccgagggcag gtgctcgtcg aagaaggcgg ccggcaccgg ctcgaacatc ggcagacccc
    13261 gcaggtagcc gatctgttcg ggggtgaagc gaagggcctc caggtattcc agggcctgct
    13321 cgatgccggc cgcgacgagg taggagcggt tccgcggcaa gtcgcggacg tacagctcga
    13381 aggtggccgg cgcccgcatg ccggccacgt agtaccccgc catcatggtc agctcgtaca
    13441 ggtcggtgga cagggacggg ggtggggaag gggacagggg agacatggga gacagagtac
    13501 ctcagcacgt ggtgctgggg gtgcaggagg tgctggaggt gctgaaggtg ctggtgctgg
    13561 aggtgctgaa ggtgctggtg ctggaggtgc tggtgctgga ggtgctggtg ctggaggtgc
    13621 gggtg
//
LOCUS       DAOPQX010000063        13512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5324_length_13512_cov_4.359515, whole genome shotgun sequence.
ACCESSION   DAOPQX010000063 DAOPQX010000000
VERSION     DAOPQX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5324_length_13512_cov_4.359515"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKK95_05250"
     CDS             complement(<1..409)
                     /locus_tag="PKK95_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOC17652.1"
                     /translation="MTPPVIQEMLGEVRGALRAAGPGEPASAFFVPGRVEVLGKHTDY
                     AGGRSLVAAVQQGFVVAARPRPDRLAVVLDVEGGRRAELALDPALPEQAEAWARYPAT
                     VVRRVARNFPEAATGADLAFLSNLPHASGMSSSS"
     gene            complement(406..1236)
                     /locus_tag="PKK95_05255"
     CDS             complement(406..1236)
                     /locus_tag="PKK95_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOC17653.1"
                     /translation="MTVSSTSCARAVILARGLGTRMRRPAEGAALDPRQASVADTGVK
                     AMIPIGRPFLDYLLTAVADAGCREACLVIGPEHDAIRKYYTEQVAVSRLAVRFAVQEH
                     PLGTADAVLAAERCTAGEPFLTLNSDNFYPVGVMARLCGLDGQGLAGFEREALVRQGN
                     IDEARVGKYAVLDVDASGALRRIVEKPDEATWDRIGRDAPISMNCWRFGPAIFDAARA
                     IGPSPRGELEIADAVQYAIDVLGERFEVVPAREGVLDLSMRADIPSVARRLEGFEVRL
                     "
     gene            1375..1764
                     /locus_tag="PKK95_05260"
     CDS             1375..1764
                     /locus_tag="PKK95_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17654.1"
                     /translation="MAKKRSTKSSGTPKRAAKKSVKKTAKKTARKAAVKKTARKAAKK
                     AAPRKAAKKAAPKKAMKKTAPKKAVKKAAPKKAAAKKAAPQKAAPKKAAPRRRVAKPA
                     PAAPPVETPAQAPVEAPPAPAPDTPVA"
     gene            1937..2923
                     /locus_tag="PKK95_05265"
     CDS             1937..2923
                     /locus_tag="PKK95_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17655.1"
                     /translation="MTIAELVRAAAERQGTRAAARLMSILADAPHRLPEVYHAAAALA
                     DQGSALELPRLLLGLTGAPGSGKSTLADALVREWRRRHPDRRVGVVAVDPSSPFTGGA
                     VLGDRVRMTRHATDPMVYVRSMASRGHLGGLALGVKGVVRVMGVVGCDAVVLETVGVG
                     QSEVEIAGVADLVVVVLAPGLGDSIQLLKAGLMEVGHLFVVNKADRPDAEKLHAELVS
                     MLRLAGPGGADGRTYLVSAEDGRGVEALTDGIEALAGRHGDEWQARRRRVIEEEVREA
                     ILEQAAGRLRATLARLPREADAVAGVLEGRVSVGEAASQLLRETCRSNHSKP"
     gene            complement(3043..4101)
                     /locus_tag="PKK95_05270"
     CDS             complement(3043..4101)
                     /locus_tag="PKK95_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC17656.1"
                     /translation="MTEDRFRKTFLLLVVGGISLLFFAMIRAFLLTLLLAAILAGLMF
                     PVYRWIERRVGGRSNLASFLTLIVMILAIFGPLGTLAGIVVQQALTVTQDLGATLKPF
                     IDDPQSIGERLALLPGADRLMPYLPRVAERGAEIVAGLGTFLVGLASAATSGTVVFLF
                     HFAILLYALFFFFSNGPRYLRTVLSYLPFSEEDNARLLERFVSVTRATLKGTLFIGVI
                     QGTINGFAFWLVNLPAPVFWGVVMIILSVVPAVGGALVWVPAAVWLAVTGRFLEAIVL
                     SVICGAVSGSVDNLLRPRLVGRDTKMPDLLILISTLGGLGFFGAIGFIIGPLVAALFL
                     TLWEILGELYRPEPRVRT"
     gene            4212..6347
                     /locus_tag="PKK95_05275"
     CDS             4212..6347
                     /locus_tag="PKK95_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC17657.1"
                     /translation="MRRRGSWILAAALALLALPAAVAQQPYPATRKSDHVDVYHGVKV
                     PDPYRWLEDDYSPETAKWVEAQNQVTFAYLEKIPFRAALKARLETLYDYERYSSPFRK
                     GPYFFFSKNDGLQNQNVLYVQRGLSSTPEVLLDPNAWSPDGTVRLTAFAPSRDASRAV
                     FGISRSGSDWQEYQVLDLAARKPLADKLEWIKVSGVAWHGDGFYYSRYPAPEKGHELS
                     AANVNHQVYYHRVGTAQSQDRLVFEDRANPQRFHIVETTEDERFAILTVSDRGQGKKG
                     NSLHVRDLSAGEAAFSPLVPEIGDDSFSVIDNVGDKLLVFTDRAAPNGRVVLMDPKNP
                     AEANWKTILPEKPEPLQMATTAGGKLFAGYLKDVATRVSVHALDGTLEGEVALPGLGT
                     CGGFAGQRDDTFVFYTYTSFDTPPTIYRYDIATRKSELFRAPRVPGYDPSAFETRQVF
                     YTSKDGTRIPMFLVHKRGLELNGANPTLLYGYGGFNVVQAPSFSPLRLALLEQGVVYA
                     SANLRGGGEYGERWHEAGTKLKKQNVFDDFIAAAEWLIANKYTAPAKLAIQGGSNGGL
                     LVGAVMNQRPELFRVAIPQVGVMDMLRFHKFTIGWNWIADYGSSDNPDEFKALLAYSP
                     LHNIKAAKYPATLVTTADHDDRVVPAHSFKYAATLQEKASRENPVLIRIETKSGHGAS
                     STKKAIETAADIYAFIFHNLGVVPRLSEP"
     gene            complement(6423..12062)
                     /locus_tag="PKK95_05280"
     CDS             complement(6423..12062)
                     /locus_tag="PKK95_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOC17658.1"
                     /translation="MSVEFQRLAILSRGEPAMRAIHAVREFNLERGTSIRTIALFTEP
                     DRRARFVREADESFALGPATFLDAADGRRKAAYLDFPRLEQALTAIQAEAVWPGWGFV
                     AERADFVELCERLGMLFVGPDSRMLRRVADKVSAKRVAEEAGLPVIPWGGEAAATLDM
                     AWLHAQRLGYPVLVKAAAGAGGRALRLAAGERELRAAYDSARREAERSFGDPTVFIER
                     YLQGVRHVEVQVQGDRHGTLQALGIRDCSIQRRFQKMIAEAPSPILETADVAALEDAA
                     LRFCRAADYRDQATIEFLFDPKTRQFAFMEVNPRLQVEHAVTEHTTGLDLVKLELDVC
                     RGGRLEGEPPATRGHAIEVRLNAENVEAGFAAAPGEIELFRLPTGPGLRVDTGVAEGD
                     LIPAEFGPMFAKLSARGHTREEALGRLKRALAESAIAIKGGASNRSLLLHLLDRPEVR
                     AAETDTGWLDRATSRPEGETGPYAGIALLQAAIEVYDRESETELEEFFSSAARMRPTV
                     RAEVGRSVEVGYLGNRYQFKVFRQGTQEYRVEVGSTRIDLLVERLGPCERWITHGDNR
                     YRVLSQVDGESHLVEVEGIPHRISRADAGLVRAPGPAVVVAVTVHPGERVAAGDRLAV
                     LESMKMEMAVSAPFAGIVRQVLVLPNAQVGAGAPLVHVEAVGLDAGEQPGARVTFEGV
                     ALAPERHEKRVSRARAILRGVKAGLGELKRGPGARVPAVLQNLRRLMLGFDLDPAETK
                     RLVGEYLRASQALDEIDPEQRRAEDELLRIFVDISAVFARQAGVEGEGRLSAEEYLYT
                     YLRSLDVRGAGLPSSFLEALQRAVAHYGVEGAEPTPQLRESLLWMFKSRRRVDQQVHA
                     VTAVLQQRLARGDGSDVPGFRSLLERLIDVSRQRFPAVAELARDVRYRTFDRPTYEEG
                     RRSVYGAVEAHLAALARDPYCADRRERLVAMVECPQPVKELFASRFEQAPPVVRQVML
                     EVLCRRYYRIRELGPFTAIETGLRSVAVTGYRHEGRSVRLLATHGTDQDLRRTIQAVQ
                     RAILAFPTDEDAIVDLHVWTRKTVEHADAASDEIRQALDEAGFDRPVRRIVVMVSGPS
                     VLAPSGQTLYFTFRPGPDGFREQKLYRGMHPMMAKRLELWRLRHFFVNRLPAAEDVYL
                     LHGVARDNPKDERLFAIAEVRDATPARDAEGRVVGLPQLERMAMEALTAIRQAQATRP
                     PAGRLQWNRVLLYVRPALYVAREDLEQVARRLAEEAEGLGLQKMVVRARIPDESGELR
                     DTIVRVALASGHGPSLEFREPTADPIQPLTEYAQKVVRMRQRGLSYPYELIRMLTPGR
                     DGVQSDLPPGAFVEHDLDADGKLVPVDRPYGRNTANVVVGVVSNFTSRYPEGMRRVII
                     LGDPSKEVGSLAEPECRRILAALDLAEQERIPLEWFALSAGAKISMESGTENMDWIAR
                     VLRRLIEFTQAGHEINIIVIGINVGGQPYWNAEATMLMHTRGILVMTAEGAMVLTGKT
                     ALDYSGSVSAEDNQGIGGYEHIMGPNGQAQYFARDVGEACRILFRHYDHAYVLPGERF
                     PRRAPTSDPIGRDVREYPHGNIQGTGFATVGDVFSDEKNPGRKKPFDIRRVLRAVSDQ
                     DHEPLERWAHLRDGENAVTWDAHVGGYPVALLGFESRPVPRVGIVPPDGPQQFTSGTL
                     FPVASRKVARFINAASGVRPLVILANLSGFDGSPESMRRRQLEFGAEIGRAVVNFKGP
                     VVFVVISRYHGGAFVVFSKALNENMEVVALEGTYASVIGGAPAAAVVFSREVDARTRK
                     DPRVAALQRELEAATDPAKAAALRARLFELSRTVRSEKLGEVAEQFDAIHSVHRALEV
                     GSLDRIIRPADLRPYVVEAIERGMARELQRVGAWNRA"
     gene            12316..12504
                     /locus_tag="PKK95_05285"
     CDS             12316..12504
                     /locus_tag="PKK95_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17659.1"
                     /translation="MTDNPKMWRRDQDLVSDTTDEIQEEVNQERIPKYSNPNRDRARG
                     DWDRSRRRTDGDLGLSEG"
     gene            12617..>13512
                     /locus_tag="PKK95_05290"
     CDS             12617..>13512
                     /locus_tag="PKK95_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOC17660.1"
                     /translation="MRLFVRLLLVLTFVCPSGVAAVASAQTVSLTILHTNDTHGHLSP
                     FSYPDAMRRQIEGLPSGGNIGGIARRATLVEQIRRDVGARGGTTWLVDVGDFSDGTPF
                     SIAYKGEADVAAMNAAGYDLGTLGNHEFNYPAAHTRRLISLAKYQLLCANVTDRTTRE
                     PLLPPSAVRRAGPVRVGVFGLITREAASYPAGREAFDVAAEIDTARRVVAALRRQSDI
                     VILLSHAGEAMDERLAAEVPGIDVIVGGHSHSRLPSGRFVWHSEDLSASDVNGTIVVQ
                     AHQWGGELGRLDLLLARDRKGA"
BASE COUNT         2093 a         4892 c         4635 g         1892 t
ORIGIN      
        1 cgctcgagct gctcatgccg gaggcgtgcg gcaggttgct caggaaggcc aaatcggcgc
       61 cggtggccgc ctcgggaaag ttgcgcgcca cgcgccggac gacggtcgcc gggtagcgag
      121 cccaggcctc ggcctgctcg ggcagcgcgg ggtcgagcgc cagttcggca cggcggccgc
      181 cctcgacgtc gagaacgacg gccaggcggt cgggccgggg tcgggcggcg acgacgaagc
      241 cctgctgcac tgcggcaacg aggctgcgcc cgcccgcgta gtcggtgtgc ttgccgagca
      301 cctcgacgcg gcccggcacg aagaacgccg acgccggctc gcccggtccg gccgcgcgca
      361 acgcgcctct cacctccccg agcatctcct ggatgactgg cggggtcaca ggcgcacctc
      421 gaagccttcg aggcggcgcg ccaccgacgg gatgtcggcg cgcatcgaca gatcgaggac
      481 gccctcgcgg gcgggcacca cttcgaaccg ctcgccgagg acgtcgattg catactgcac
      541 ggcgtcggcg atctcgagct cgccgcgcgg cgatgggccg atggcgcgcg cggcgtcgaa
      601 gatcgccgga ccgaaccgcc agcagttcat gctgatgggc gcgtcgcgcc cgattcggtc
      661 ccacgtcgcc tcgtccggct tttccacgat gcgccgcagg gcgcccgacg cgtccacgtc
      721 gagcacggcg tacttgccga cgcgcgcctc gtcgatgttg ccctggcgca ccagggcctc
      781 gcgctcgaaa ccggcgagcc cctgcccgtc gaggccgcac aagcgcgcca tcacgccgac
      841 cgggtagaaa ttgtcggagt tgagcgtgag gaacggctct cccgcggtgc agcgctctgc
      901 cgcgagcacg gcatccgcgg tgccgagcgg gtgctcctgc acggcgaacc gcacggcgag
      961 ccgcgagacc gccacctgct ccgtgtagta cttgcggata gcgtcgtgct cgggcccgat
     1021 caccaggcac gcctcgcggc agcccgcgtc ggcgacggct gtgagcaggt aatcgaggaa
     1081 cggccggccg atcgggatca tcgccttgac cccggtgtcc gcgacactgg cctggcgggg
     1141 atcgagcgcg gcgccctcgg cgggacgccg catgcgggta ccgagaccgc gcgccaggat
     1201 gaccgcgcgc gcgcacgacg tcgaactcac tgtcaccgcc ggacgataac gcatcgcctt
     1261 cgggcggatc aagccgcacc ctcgtgcgat ggatcatttc gctcgctcga gatgagctat
     1321 aatcacgccg gcctaatcgc cgaaacgatc ttcggcgttg tgaaggaggg acagatggcg
     1381 aagaagcgga gcacgaagtc atcggggacc ccgaagcgag cggccaagaa gtcggtgaag
     1441 aagacggcga agaagacggc gcgcaaggct gccgtgaaga agaccgcgcg gaaggccgcc
     1501 aagaaggccg ccccgcggaa ggccgccaag aaggccgccc cgaagaaggc catgaagaag
     1561 accgccccca agaaggcggt caagaaggcc gccccgaaga aggctgccgc gaagaaggct
     1621 gccccgcaga aggccgcccc gaagaaggcc gccccgcgcc gccgcgtggc caagcccgct
     1681 cccgcggccc cgccggtcga gacgccggcc caggccccgg tcgaggcgcc tccggcgccg
     1741 gctcccgaca cgccggtcgc ctgacgccgc tcgtccccct gccggcagac aggcgcgggc
     1801 tgcatcccgg ccgttccgtc ccgggatgcg gcccttccca ccgaggccga tccgcgcctc
     1861 gccccggcgc gagcccgcgt gcctctcggt cgcgtcgatt cctgtacact gatcgggcgc
     1921 ccacaagccg tccgacatga ccattgccga actcgttcgc gccgccgcgg agcgccaagg
     1981 cacgcgggcc gctgcgcgcc tgatgtcgat cctcgccgac gccccgcacc ggctccccga
     2041 ggtctatcac gcggccgctg cgctcgccga ccagggctcc gctcttgaac tgcccaggct
     2101 gttgctcggt ctcaccgggg cgccgggctc gggcaagagc accctggccg acgcgctcgt
     2161 gcgcgagtgg cgccgacgcc atcccgatcg ccgggtcggc gtcgtggccg tcgacccctc
     2221 gtccccgttc actggcggcg ccgtcctcgg cgaccgcgtc cggatgacgc gccacgccac
     2281 cgacccgatg gtttacgtcc ggtcgatggc ctccaggggg cacctcggcg gcctcgccct
     2341 cggcgtcaag ggggtggtgc gcgtgatggg ggtggtaggc tgcgacgcgg tcgtcctcga
     2401 gacggtcggc gtcggtcaga gcgaggtcga gatcgcgggg gtcgccgacc tcgtcgtcgt
     2461 cgtcctcgcc ccgggcctcg gcgacagcat ccaactgctc aaggccgggc tgatggaggt
     2521 cggccacctg ttcgtcgtca acaaggccga ccggcccgac gcggagaagc tgcacgccga
     2581 gctcgtctcc atgcttcggc tggcgggccc cgggggcgcc gacggccgaa cctacctggt
     2641 gagcgccgag gacggccggg gcgtcgaggc gctcaccgac ggcatcgagg ccctcgccgg
     2701 ccgccacggt gacgagtggc aagcgcggcg ccggcgggtg attgaagagg aggtgcggga
     2761 ggcgatcctc gagcaggcgg cgggccggct tcgggccacg ctcgctcgtc tcccgcgcga
     2821 ggcggatgcg gtggccggcg ttctcgaggg ccgcgtttcg gttggcgaag ccgccagtca
     2881 gctgctgcgg gaaacctgcc gctcgaacca ctccaagccc tgagttcaga gacttctggc
     2941 atgatctgaa tcctctgcgt gcgtctccgc gtctctgtgc tctctgtggt taacgtgggc
     3001 aagccacccc gccgactgtc acgcacgttt catgtgggca acctaggttc tcaccctcgg
     3061 ctcgggcctg tacagctccc cgagaatctc ccagagcgtc aggaagaggg cggcgaccag
     3121 cgggccgatg atgaagccga tggcgccgaa gaagccgagc cccccgagcg ttgagatcag
     3181 gatgaggagg tcgggcatct tggtgtcgcg gccgaccagc ctcgggcgca gcaggttgtc
     3241 cacgctgccc gacaccgccc cgcagatcac cgagaggacg atggcctcga gaaaccgccc
     3301 ggtgaccgcc agccacacgg cggcgggcac ccacacgagc gccccgccga cggccggcac
     3361 gaccgagagg atgatcatga ccacgcccca gaagacgggg gccgggagat tcaccagcca
     3421 gaaggcgaag ccgttgatcg ttccctggat gacgccgatg aacagcgtcc ccttcagcgt
     3481 cgcgcgggtg accgagacga accgctcgag aaggcgcgcg ttgtcctcct cgctgaacgg
     3541 caagtacgag aggaccgtgc ggaggtagcg cggcccgttg ctgaagaaga agaagagcgc
     3601 gtacagcagg atcgcgaagt ggaagaggaa gaccacggtc ccgctggtgg cggccgaggc
     3661 gaggcccacc aggaacgtcc cgaggcccgc gacgatctcc gcgccgcgtt cggccacgcg
     3721 gggcaggtac ggcatcaggc ggtcggcgcc cggcagcagg gcgaggcgct ccccgattga
     3781 ctgggggtcg tcgatgaacg gcttcagggt ggcgccgagg tcctgtgtca cggtcaacgc
     3841 ctgctggacg acgatgcccg caagcgtccc gagtgggccg aagatcgcga ggatcatcac
     3901 gatcagcgtg aggaacgacg ccaggttgga gcgccccccc acccggcgct cgatccaccg
     3961 gtacaccggg aacatcaggc cagcgaggat cgccgcgagc agcagcgtca ggaggaaggc
     4021 gcgaatcatc gcgaagaaga gcagcgagat cccgccgacg accagcagca ggaacgtctt
     4081 gcggaagcgg tcctcggtca ccgcttcatg gtaacgtgga ggcctgtcaa cgtgacattt
     4141 tccttttccg gtgccgccgc cccggaaacg ccaggggttc cgcctcccct gcccgcgcaa
     4201 ggagttgcac gatgagacgc cgaggttctt ggattctcgc cgccgccctc gccctgctgg
     4261 ccctgccggc tgccgtcgcc cagcagccct accccgccac gcgcaagtcc gatcacgtgg
     4321 acgtctacca cggggtcaag gtgccggatc cgtaccggtg gctcgaggac gactactccc
     4381 ccgaaacggc gaagtgggtc gaggcgcaga accaggttac ctttgcctac ctcgagaaga
     4441 tcccgttccg cgccgcgctc aaggcccggc tggaaaccct ctacgactac gagcgctact
     4501 cgtcaccgtt ccgcaagggg ccgtactttt tcttcagcaa gaacgacggc ctgcagaacc
     4561 agaacgtcct ctacgtccag cgcgggctct ccagcacgcc cgaggtgctc ctcgatccca
     4621 acgcctggtc tcccgacggg acggtccggc tgacggcctt cgccccgtcc cgggacgcgt
     4681 cgcgcgccgt gttcggcatc tcgcgcagcg gctccgactg gcaggagtac caggtgctcg
     4741 atctggccgc ccggaagccg ctcgccgaca agctcgagtg gatcaaggtg tccggcgtgg
     4801 cgtggcacgg cgacgggttc tactacagcc gctacccggc gcccgagaag gggcacgagc
     4861 tgtcggccgc caacgtgaac caccaggtgt actaccaccg ggtcggcacg gcgcagtcgc
     4921 aagaccggct cgtcttcgag gatcgcgcca acccgcagcg cttccacatc gtcgagacca
     4981 ccgaggacga acgcttcgcg atcctcaccg tgtcggatcg cggccagggc aagaagggca
     5041 actccctgca cgtcagggac ctctcggcgg gcgaggccgc gttcagcccg ctcgtccccg
     5101 agattggcga cgacagcttc agcgtgatcg acaacgtcgg cgacaagctg ctcgtcttca
     5161 ccgatcgcgc ggcgcccaac ggccgggtgg tcctgatgga tcccaagaac ccggccgagg
     5221 cgaactggaa gacgatcctc ccggagaagc cggagccgct ccagatggcc acgacggccg
     5281 gcgggaagct gttcgcgggc tacctgaagg acgtcgccac gcgcgtttcg gtgcacgccc
     5341 tcgacgggac gctcgagggc gaggtcgcgc ttcccggcct gggcacctgc gggggcttcg
     5401 ccggccagcg cgacgacacg ttcgtcttct acacctacac gtcgttcgac acgccgccca
     5461 ccatttaccg gtacgacatc gctacccgga agagcgagct gttccgggcg ccgagggtcc
     5521 ccggttacga cccgtcggcc ttcgagacca ggcaggtctt ctacaccagc aaggacggga
     5581 cgcgcatccc gatgttcctc gtccacaagc gtggcctcga gctgaacggg gccaacccga
     5641 cgctcctcta cgggtacggc ggcttcaacg tcgtgcaggc gccctcgttc agcccgctgc
     5701 gcctggcgct gctcgaacag ggggtcgtct acgcctcggc gaacctccgc ggcggcggcg
     5761 agtacggcga gcgctggcac gaggccggaa ccaagctgaa gaagcagaac gtgttcgacg
     5821 acttcatcgc cgctgccgag tggctgatcg cgaacaagta cacggcgccc gcgaagctgg
     5881 ccatccaggg cgggtcgaac ggcggcctgc tggtcggcgc cgtcatgaac cagcggccgg
     5941 agctgttccg cgtggccatc ccgcaggtcg gcgtgatgga catgctccgc ttccacaagt
     6001 tcacgatcgg ctggaactgg atcgccgact acggatcgag cgacaacccg gatgagttca
     6061 aggcgctcct cgcgtactcg ccgctgcaca acatcaaggc ggcgaagtac ccggccacgc
     6121 tcgtcacgac ggccgatcac gacgaccgtg tcgtgccggc tcactcgttc aagtacgcgg
     6181 cgacgctgca ggagaaggcg agccgggaga acccggtgct gatccggatc gagaccaagt
     6241 cgggccacgg ggcgagcagc acgaagaagg cgatcgagac ggccgccgac atctacgcgt
     6301 tcatcttcca caacctggga gtcgtcccca ggctgtcgga accgtaagga tgtcaccgcg
     6361 ccgcagggac gagcacacct ccgcttcagc ttcggcgtgg tcgcccctgc gaggcggcgc
     6421 cgctacgcgc ggttccacgc ccccacgcgc tgcagctcgc gcgccatccc tcgctcgatc
     6481 gcctcaacga cgtacggccg caggtcggcc ggcctgatga tgcgatcgag cgaaccaacc
     6541 tccagcgccc ggtggacgct gtggatcgcg tcgaactgct cggctacctc gcccagcttc
     6601 tcggaccgga cggtgcgaga gagctcgaac agccgggcac gcagagcggc ggccttcgcc
     6661 gggtcggtgg ccgcctcgag ctcgcgctgc agcgcggcga cgcgcgggtc cttccgcgtc
     6721 cgggcgtcca cctcgcgcga gaagaccacc gcggccgccg gcgccccgcc gatcaccgac
     6781 gcgtaggtgc cctcgagcgc caccacctcc atgttctcgt tgagcgcctt cgagaacacg
     6841 acgaaggcgc cgccgtggta gcgcgaaatc acgacgaaga cgaccgggcc cttgaagttc
     6901 acgaccgcgc gcccgatctc ggcgccgaac tcgagctgcc gccgccgcat cgactcgggc
     6961 gacccgtcga agcccgacag gttcgccagg atcacgagcg gccggacgcc gcttgccgcg
     7021 ttgatgaacc gcgccacctt gcgcgacgcc accgggaaca gcgtgcccga ggtgaactgc
     7081 tgggggccgt cgggcggcac gatcccgacc cgcggaaccg gcctcgactc gaacccgagc
     7141 agcgccaccg ggtagccgcc gacgtgcgcg tcccaagtga cggcgttctc cccgtcgcgc
     7201 aggtgcgccc accgctcgag cggctcgtgg tcctggtcgc tcaccgcccg caggacccgg
     7261 cggatgtcga acggcttctt ccgccccggg ttcttctcgt cggagaagac gtcgccgacc
     7321 gtggcgaacc ccgtgccctg gatgttcccg tgcgggtact cgcgcacgtc gcggccgatt
     7381 gggtcggagg ttggcgcgcg gcgcgggaag cgctcgcccg gcagcacgta ggcgtggtcg
     7441 tagtgccgga agaggatccg gcaggcctcg ccgacgtcgc gggcaaagta ctgggcctgc
     7501 ccgtttggcc ccatgatgtg ctcgtacccg ccgatcccct ggttgtcctc ggccgagacg
     7561 gagccggagt agtcgagggc ggtcttcccc gtcagcacca tcgctccctc ggccgtcatc
     7621 accaggatcc ccctcgtgtg catcagcatc gtcgcctcgg cgttccagta cggctggccg
     7681 ccgacgttga tgccgatgac gatgatgttg atctcgtggc cggcctgggt gaactcgatc
     7741 agccgccgca gcacccgcgc gatccagtcc atgttctcgg tgccgctctc catcgagatc
     7801 ttggcgccgg ccgagagcgc gaaccactcg aggggaatgc gctcctgctc ggccaggtcg
     7861 agggcggcga ggatgcgccg gcactcaggc tccgccagcg accccacctc cttgctcgga
     7921 tcgccgagga tgatcacgcg ccgcatgccc tccgggtagc ggctggtgaa gttggacacc
     7981 acgccgacga ccacgttcgc cgtgttgcgg ccgtagggcc ggtcaaccgg caccagcttc
     8041 ccgtccgcgt cgaggtcgtg ctcgacgaag gcgccggggg gcaggtccga ctgcacgccg
     8101 tcgcgcccgg gggtgagcat gcggatcagc tcgtacgggt agctcagccc tcgctggcgc
     8161 atccgcacga ccttctgggc gtattcggtc aacggctgga tcgggtcggc ggtgggctcg
     8221 cggaactcca gcgacgggcc gtggcccgat gcgagggcga cccggacgat cgtgtcgcgt
     8281 agctcgcccg attcgtcggg gatgcgcgcc cggacgacca tcttctggag ccccagcccc
     8341 tcggcctcct cggcaaggcg ccgcgccacc tgctccaggt cctcgcgcgc cacgtagagc
     8401 gcgggccgga cgtacagcag cacccggttc cactgcagcc gcccggcggg cggccgcgtc
     8461 gcctgcgcct gccggatcgc cgtgagcgcc tccatggcca tccgctcgag ctgcggcagc
     8521 ccgaccacgc gcccttccgc gtccctcgcc ggcgtcgcat cgcgcacctc cgcgatcgcg
     8581 aacaaccgct cgtccttggg gttgtcacgc gccacgccgt gcagcagata aacgtcctcg
     8641 gccgccggga ggcggttcac gaagaagtgg cgcaggcgcc acagctcgag ccgcttggcc
     8701 atcatcgggt gcatcccgcg gtacagcttc tgctcgcgga acccgtcggg accggggcgg
     8761 aacgtgaagt acagcgtctg gcccgagggc gcgagcaccg acggccccga gaccatcacc
     8821 acgatgcgcc tcacgggccg gtcgaacccc gcctcgtcga gcgcctgccg aatctcgtcg
     8881 gacgccgcgt cggcgtgctc caccgtcttg cgcgtccaga cgtgcagatc cacgatggcg
     8941 tcctcgtcgg ttgggaacgc gaggatcgcc cgctgtaccg cctggatggt ccggcgcagg
     9001 tcctggtcgg tgccgtgcgt cgccagcagg cggacgctgc gcccctcgtg gcggtagccc
     9061 gtcaccgcca cgctgcgcag cccggtctcg atggcggtga agggcccgag ttcccggatc
     9121 cggtagtagc gccggcacag gacttcgagc atcacctggc gaacgaccgg cggcgcctgc
     9181 tcgaagcgcg acgcgaacag ctccttgacc ggctgcgggc actccaccat ggcgaccagg
     9241 cgctcacggc ggtcggcgca gtaggggtcg cgcgccagcg cggccaggtg agcctcgacc
     9301 gcgccgtaca ccgagcggcg gccttcctcg tacgtcggac ggtcgaaggt gcggtaccgc
     9361 acgtcgcggg ccagctcggc gacggccgga aaccgctgcc tcgacacgtc gatcaggcgc
     9421 tcgagcaggc tccggaagcc ggggacgtcg gacccgtcgc cgcgggcgag ccgctgctgc
     9481 agcaccgccg tcaccgcgtg cacctgctgg tcgacgcggc gacgcgactt gaacatccac
     9541 agcaggctct cgcgcagctg cggcgtcggc tcggccccct cgacgccgta gtgggccacc
     9601 gcccgctgga gcgcctcgag gaagctcgac ggaaggcccg ccccccggac gtcgagcgag
     9661 cgcaggtagg tgtagaggta ctcctcagcg ctcaggcgtc cttcgccctc gactccggcc
     9721 tggcgcgcga acacggcgga gatgtcgacg aagatccgga gcagctcgtc ctcggcccgg
     9781 cgctgctcgg ggtcgatctc gtcgagcgcc tggcttgccc gcaggtactc gccgaccagc
     9841 cgcttggtct cagcgggatc gaggtcgaag cccagcatga gccggcgcag gttctgcagc
     9901 accgcgggaa cgcgggctcc cggcccgcgc ttcagctcgc caaggcccgc cttgacgccg
     9961 cggaggatcg cgcgggcccg tgagacccgc ttctcgtgcc gttccggtgc gagcgccact
    10021 ccctcgaagg tcacgcgcgc gcccggctgc tcgcccgcgt cgaggccgac cgcctcgacg
    10081 tgcacgagcg gcgccccggc cccgacctgc gcgttcggca gcaccagcac ctgccggacg
    10141 atgcccgcaa agggagccga gaccgccatc tccatcttca tcgactcgag gacggcaagc
    10201 cgatcgcccg cggccacccg ctcgcccggg tgcacggtga ccgccacgac gacggccggg
    10261 cccggcgccc gaacgaggcc ggcatcggcc ctggagatcc ggtgcgggat cccctcgacc
    10321 tccacgaggt gcgattcgcc gtccacctgc gagagcacgc ggtagcggtt gtcgccgtgg
    10381 gtaatccacc gctcgcaggg gccgagccgc tccacgagca ggtcgatccg cgtggacccg
    10441 acctcgacgc ggtactcctg ggtgccctgc cggaagacct tgaactgata gcggtttcca
    10501 aggtaaccga cctccaccga ccggccgacc tcggcgcgga cggtgggtcg catgcgcgca
    10561 gccgacgaga agaactcctc gagctcggtc tccgactcgc ggtcgtagac ctcgatggcc
    10621 gcctgaagga gcgcgatgcc ggcgtacggg ccggtctccc cctccggccg gctcgtcgcc
    10681 cgatcgagcc agccggtgtc cgtctcggcg gcgcgcacct cggggcgatc gagcaggtgc
    10741 agcagcaggc tccggttgct ggcgcccccc ttgatcgcga tggcgctctc ggccagcgcc
    10801 cgtttcagac ggccgagcgc ctcctcgcgg gtgtgccccc gggccgagag cttcgcgaac
    10861 atcggcccga actcggccgg gatgaggtca ccctccgcaa ccccggtgtc gacccgcagg
    10921 ccagggcctg tcggcagtct gaagagctcg atctcgccgg gcgccgccgc gaaaccggcc
    10981 tccacgttct cggcgttcag ccgcacctcg atcgcgtggc cccgggtggc tggcggctcg
    11041 ccctcgagac ggcctccgcg gcagacgtcc agctccagct tgaccaggtc gaggccggtc
    11101 gtgtgctcgg tgacggcgtg ctcgacctgc agccgcgggt tcacctccat gaaggcgaac
    11161 tggcgcgtct tcggatcgaa caggaactcg atggtggcct ggtcacggta gtcggcggct
    11221 cggcagaagc ggagggcggc gtcctcgagc gcggccacgt cggccgtctc caggattggc
    11281 gacggggcct cggcaatcat cttctggaag cgccgctgga tcgaacagtc gcggatgccc
    11341 agcgcctgca gcgtgccgtg gcggtcgccc tgtacctgca cctccacgtg gcgcacgccc
    11401 tgcaggtacc gttcgatgaa gacggtggga tcgccgaagc tgcgttcggc ctcgcggcgt
    11461 gcgctgtcgt aggcggcgcg cagctcgcgc tccccggcgg cgagccgtag cgcgcggccg
    11521 cccgcgcccg ccgccgcctt gacgagcacc gggtagccga gccgctgcgc gtgcagccag
    11581 gccatgtcga gggtggcggc ggcctcccct ccccacggga tgaccggcag gccggcttcc
    11641 tccgcgactc gcttggccga caccttgtcg gccacgcggc gaagcatccg gctgtcgggg
    11701 ccgacgaaga gcatccccag gcgctcgcac agctccacga agtcggcgcg ctcggcgacg
    11761 aagccccagc cgggccacac ggcctcagcc tggatggccg tcagcgcctg ctcgagccgg
    11821 gggaaatcga ggtaggcggc cttgcgccgc ccatcggcgg cgtcgaggaa ggtggccggg
    11881 ccgagagcga acgattcgtc ggcctcgcgc acgaaccgcg cgcggcggtc gggctcggtg
    11941 aacagggcga tcgtccggat cgaggtgccc cgctcgaggt tgaactcgcg gacggcgtgg
    12001 atggcgcgca tcgccggctc cccacggctg aggatggcca ggcgctggaa ctcaacactc
    12061 atcgtgacct catcgcatac gactgcgcat accactgccg gagcgcatcg accggcgggg
    12121 ctttcggggc cagccattct accttggatc aacggctcag ccgcctcggc ggacacgtgg
    12181 tgccggcccg ctgacgcagg cgcgcggcgc gctttccttc cggcgctaaa tccggccagt
    12241 tctggaggga ggcccggagg cacatttctt ggacaggttc cacgaccggc tgtcatgacc
    12301 acgcatggag ggagcatgac cgacaatccg aagatgtggc ggcgcgacca ggatctggtc
    12361 agcgatacca ccgacgagat ccaggaagag gtgaaccagg agcgcatccc gaagtactcc
    12421 aaccccaatc gcgaccgggc acggggcgac tgggatcgca gccgccgccg caccgacggc
    12481 gacctcggcc tctcggaagg ctgattcatt cgaccgggtg gtaggggcgg gcgcccgccc
    12541 gtaccacccg gctgttcacc gccgcctccc tcctccagta caattccttc cccgtgtgcc
    12601 ctctccaggt gatctcatga gactgttcgt ccggttgctg ctggtcctga cattcgtctg
    12661 cccgtccggc gtcgccgccg tcgcctcggc ccagaccgtt tcgctcacca tcctccatac
    12721 caacgacacg cacgggcacc tgtcgccctt ctcctatccc gacgccatga ggcgccagat
    12781 cgaggggctg ccgtccggcg ggaacatcgg cggaatcgcc cggcgcgcca cgctggtcga
    12841 gcagatccgg cgcgacgtcg gcgcccgcgg cggcaccacc tggctggtgg acgtgggcga
    12901 cttcagcgac ggcaccccgt tctccatcgc gtacaagggc gaggccgacg tggcggccat
    12961 gaacgcggcc ggctacgacc tcggcacgct gggaaatcac gagttcaact atccggccgc
    13021 gcacacccgg cgcctgatct cgctggcgaa gtaccagctg ctctgcgcga acgtgaccga
    13081 ccgcaccacc cgcgagccgc tcctgccgcc gtcggccgtt cggcgggccg ggccggtacg
    13141 ggtcggggtc ttcggcctca tcacgcgcga agcggcgtcg tatcccgccg gccgggaagc
    13201 cttcgacgtt gccgccgaga ttgacacggc acgccgggtg gttgcggccc ttcgccgcca
    13261 atcggacatc gtcatcctgc tgtcgcacgc cggcgaggcg atggacgagc ggctggccgc
    13321 cgaggtcccc gggatcgacg tgatcgtggg ggggcactcg cactcgcgcc tgccgtcggg
    13381 gcggttcgtc tggcactcgg aggacttgag cgcctccgac gtcaacggca cgatcgtcgt
    13441 gcaggcgcac cagtggggcg gcgagctggg gcggctcgac ctgctgctgg cccgcgaccg
    13501 gaaaggggcg tg
//
LOCUS       DAOPQX010000064        13214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5461_length_13214_cov_5.028422, whole genome shotgun sequence.
ACCESSION   DAOPQX010000064 DAOPQX010000000
VERSION     DAOPQX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13214
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5461_length_13214_cov_5.028422"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..603
                     /locus_tag="PKK95_05295"
                     /pseudo
     CDS             31..603
                     /locus_tag="PKK95_05295"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
     gene            600..2114
                     /locus_tag="PKK95_05300"
     CDS             600..2114
                     /locus_tag="PKK95_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC17661.1"
                     /translation="MRERLAALARSQPGRLAFAVGTHILTYGELARDAARLAAALRRA
                     GAAPGGHVAIVLPSGLDFVRAFYALDWLGAAAVPINPDLPPEQIRRRIGAAGCHLEID
                     AASFEALDEGSKSLTPLDPGPPSPDSVAYLQLTSGTSGEQRAAVILRRNLEATLRGMR
                     DMIGFGPEDVMAGSIPLHHNFGLVRFVCAPVYCGCPCHLTGAPPANVGRWLALLTEVR
                     ATVTAAPDFLYRAAALRVDPSTVDLRALRFASNGGEAVHLETIRMFEERFGVPGTVRP
                     GYGLAETTLTVSSLAPGLPLRSIDHGIPSCGPAIPGTEIAIADEDGRHLPRGERGEIV
                     VRGPSVFAGYYQDPEGTARVFRDGWLRTGDQGVLDADGFLYVTGRQRWMIKRGGATIV
                     PGEVEAAAACVPGVLRAAAVGVAAPHGTTERFVVAIEIDPQLGSDGSLLSGLAGKVAA
                     AVRQGVGHQPGEIVLLAPGSLPLTGSGKVRHGQLRDLFASGEADDRLRPLARFS"
     gene            complement(2109..3026)
                     /locus_tag="PKK95_05305"
     CDS             complement(2109..3026)
                     /locus_tag="PKK95_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17662.1"
                     /translation="MRSKIPVSARVPMAARPAWFRAAVGACDAVTRRMPATGVRLAEA
                     AGWLCHAGRLWGAAPSEVSAVFPWLPDAAAARLARRIAAQDLKCRAYDRLLYSGGIES
                     AASLLSRPHLARLEQRLREPGGQVLLDVHTGPISATSAALLLLGKTALIVRKSGRTAC
                     ARGLEVVGLEGDDQHRAQVLRRGVEYLRQGNAVVLAADGWEGAATEPVACCGRLVRFR
                     RGAFVMARLSGAPILPLYFRWGDGGRIEVEAAEPIRPAGVGDAVRQETEMAAVVAGWL
                     EGQLLARPDDLSLSLLKWLQTSGPPVAVL"
     gene            complement(3026..3313)
                     /locus_tag="PKK95_05310"
     CDS             complement(3026..3313)
                     /locus_tag="PKK95_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOC17663.1"
                     /translation="MANLVLDVDGNAREREAELVRQLVRSLAPSWTIQNVNDDTPIGP
                     SGLGLDSVAIVELLVACEERFGVAFPDQLLDVHPLTIGTLVRHLDEARAGR"
     gene            3510..3764
                     /locus_tag="PKK95_05315"
     CDS             3510..3764
                     /locus_tag="PKK95_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17664.1"
                     /translation="MAPATVVVNVVLEPDERNRALIVVTDSADFYSSSEVALDGDRSA
                     RQQRFTVRNLPAGEYVVQARIKRADNSERIAETHLVVTGF"
     gene            complement(3787..5172)
                     /locus_tag="PKK95_05320"
     CDS             complement(3787..5172)
                     /locus_tag="PKK95_05320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC17665.1"
                     /translation="MPSLTFLGAAQTVTGSKYLLEVNASRVLVDCGLFQGLKELRERN
                     WAPLPVAAESIDSVILTHAHVDHSGYLPRLVREGFRGRVFCTPGTADLCRIVLPDSGR
                     LAEEDAREANRHGYSKHSPALPLFTEEDAVRALTHLQPVGFDRPLPAAPGVTVTFTNS
                     GHLLGSAFANLELDVDGGRHVVFSGDVGRYNRPVLPDPSPLESADVLLVESTYGDRAH
                     LPDDDGVKMAEIVVRTIDRGGKVIIPSFAIGRVEEIIWWLKRLEDEGRIPSVPVFVDS
                     PMAVEALRHYARRSDELDPDLHGVRGEFASFVTRRFTTVATPQQSAELVASRIPSIVI
                     SSSGMATGGRVLNHLKSALPNPRNTVLFVGFQAAGTRGRRLAGGEREVKIHGQMVPVN
                     ASVEQLHSMSAHADGDELIRWLSGVERPPKATFVVHGEPESAETFAGRLRQELSWPAT
                     VPKYLDAIELP"
     gene            complement(5183..6031)
                     /locus_tag="PKK95_05325"
     CDS             complement(5183..6031)
                     /locus_tag="PKK95_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17666.1"
                     /translation="MSTLLETLRSEEPFVAAELRPPRAELASAAGMDAWIDTYHAVRS
                     LTLQGTSVFLTDSAVGVPEEDNLRHLTANIGDDAARDRIVPILTSKHPLEYCLSYADR
                     ARQHGFPSLVVLGGDRRIGSPRSVEHAWELRQAIRGTGSDLVLGGWANPHRPAERQVD
                     YLCDPRFTGEYFLTQVVSHHQVTPVARFLEVADARGVTMPGVFGVFYYRSANPKTLSL
                     LADFISVPAEGLTREFDEGAPPEEVCARTLRALKALGVRRFYISNLPLVGTARTLARI
                     RGLAGV"
     gene            6054..7037
                     /locus_tag="PKK95_05330"
     CDS             6054..7037
                     /locus_tag="PKK95_05330"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HOC17667.1"
                     /translation="MTFSAGLDALCALPSLSLAQYPSPIEPLSRLGAALGAAPRLLVK
                     RDDALSFGFGGNKVRKIEMLAARALDDDADVLITTGGVQSNHCRVTAAAAARLGMRCV
                     IVANGERPPRATGNALLDELFGADVQYVASREERAPAMEAIAERLRSEGMHPVIIPLG
                     ASTPLGAAGFARAVGELVSQLDEAPDYIFHSTSSGGTQAGLLAGQVLYNVKTRVIGIS
                     ADEPADRLQAAVSGIVSGLGELLGMDGRKLAAARPIEVDTRFIGEGYGVPTEASLEAQ
                     SLLARTEALVVDHTYTAKALAGLIGWVREGRFRESDTVLFWHTGGQVAVFA"
     gene            7081..8469
                     /locus_tag="PKK95_05335"
     CDS             7081..8469
                     /locus_tag="PKK95_05335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17668.1"
                     /translation="MHNRLRILHAIHDFLPRHQAGSEIYAFNLCRQLARRHFVHVLAA
                     EFDPSRPHGTLRWRVHEGIPVVELINNWETASFADTWRSAVIDRRLGQVLQATQPDVL
                     HVHSLLNLSFNLPALARARGIPVVATLHDHTLACPAGGQRLHDTEGTVCDRIDPARCA
                     GCFARSPFQAQMSVAAVGRLAGRAGVRLVEAARLVARRMPRVTAALVRSTVARRVAPV
                     SAAEVGERLAALAHVFEAVDLFVAPSEAVARAHRELGLPDEKVVVSDYGFPGGAGRQP
                     YAQAPPRPVRIGFVGTLVRHKGAHLLLEAARDWPGGRFEIEIHGSLDTFPAYSLQLTR
                     SAEGLPVRFCGPFDPSRSASIYGGLDLLVVPSLWPENSPLVIHEAFLAGVPVAAAATG
                     GIPELIADGVNGVLFEPGSARSLADAVRRLIEDPARLQRLASAKPAVKSIEADAREWE
                     QRYGALVSGRRA"
     gene            8466..9446
                     /locus_tag="PKK95_05340"
     CDS             8466..9446
                     /locus_tag="PKK95_05340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17669.1"
                     /translation="MNAGRSGPCDAPHPLASVLLLTFNGGQRLGVVLEALARQEAPFP
                     HEVVAVDSGSTDGTRERLQQAGARVISIPRGEFNHGATRNLGIEACKGVFVVLLVQDA
                     EPEGRDWLARLVDPLHEDATLAGTYDRQVPPEGAGSVPRAYLARHAAASAEPRVQQLA
                     GRAAYEALTPWQRMEACTFDNVCSCVRRSVWARRPFARAPIAEDLEWARDVMLDGYRL
                     AYVPEARVRHLHDRPASYELKRTYLVHQRLRRLFGLRTIPSAAHLAGAVLVSLASHAR
                     WVAAAAGSPWPRRLAELPRAIALAFALPFGQYLGARSADSGRDLLRVRGV"
     gene            9447..>11570
                     /locus_tag="PKK95_05345"
     CDS             9447..>11570
                     /locus_tag="PKK95_05345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17670.1"
                     /translation="MRILFVVHGFPPSATGGTEIYALDLARALQRQGHEVRVLARFAN
                     RELPEYTATRHVVSGVPVTLVNNTFLRAASFEDTYRNAAIDAIAGAAIDEYGPDVVHV
                     HHLTCLSTRIVAACRQRGIPAVITLQDYWLLCHRGQLLDLDLERCDGPAADRCSRCAG
                     YAASPSRGLHAAARTLRSIERRLPATLTAAERRLVSAASRRAVPRGASGATARRLEEA
                     RSVCRDAALLLAPSRTIFDTFQRFGVGRERMRRQDQGIDLRPFQDVTRTRGDRLRLGF
                     AGSLMVSKAPHLFIEAAAGLPRDRVEVTLAGGHAAYHGDDSYASTMRPLLEQPWVKWI
                     GPVPHEEMPRILSALDVLVVPSIWLENSPFVIREARAAGAVVVASRLGGMAEAVSNGR
                     DGLLFEPGNASSLRRALTRLLDEPELLDRLREGTRPVRSIDDDAAWTAAVYEEVRDKG
                     RASPGVRRDLAGNASGSPRIAAVVLNYRAPDDTLLAVRSLEASRRPVQPVIVVDNGND
                     GSCSRSLAAGRSRAVLMKMQSNRGFAGGCNAGIYESLRRGADMVVLLNSDAVVAPDTV
                     ERLEAALAADPGAGIAAPLVVSRSDPGTIASAGIRFSPVSGRMRQEGAGEAFDGVAGG
                     PAARVSAVSGAVMLIRRAVFEQAGLFDERYFYSFEDIEFCWRAARAGWATLLVPSALA
                     WHEGHRSIGPQSAARLYFASRNHLLL"
     assembly_gap    11571..11580
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11581..11760
                     /locus_tag="PKK95_05350"
     CDS             <11581..11760
                     /locus_tag="PKK95_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17671.1"
                     /translation="VAPSSPPASWLRGAVIVLLNAAHAARTPGVGIRQGLLAVAAGAR
                     DHLRGRYGRAPRKRA"
     gene            12096..12806
                     /locus_tag="PKK95_05355"
     CDS             12096..12806
                     /locus_tag="PKK95_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC17672.1"
                     /translation="MRVLVLVPARDEARSLPGLVHVLRAEQPGAGILVVDDASTDATR
                     QLLPGLGVEWLTLCAHTGVGGTIRAGLRYALRRGYDVVVRVDGDGQHPADQIERVLAP
                     IRAGQVDAVVGSRYRQGSGYRAPGWRRAAQRVLAAWVSAAIRQRITDPTSGFWAFGPR
                     AVRLLASHHPTGYPEPELLLLLAHNGLRVSEVPIEMRRRLAGRSSLTAARVGAIAGRT
                     ALALLILPLRRTVPDGDD"
     gene            12799..13188
                     /locus_tag="PKK95_05360"
     CDS             12799..13188
                     /locus_tag="PKK95_05360"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HOC17673.1"
                     /translation="MTNPIQLIAVAVSLALVVVVIELVRRGALTEEYSLIWLVCGAAL
                     LVLSFWKNILDVSARWLGIYYPPAVLLLVVVFFGFVASLYFSVVISGQRRRIERLIEE
                     AALIDARVRELEAEAGTREAACHAGPS"
BASE COUNT         1772 a         4730 c         4741 g         1961 t
ORIGIN      
        1 ggaagcagct gatatttcag ctcaaaacca gcgcccggcg gcggatcggt gtcggcgctg
       61 gccggcagcc ttgctgccgc gctggcagcg atggtggcca acctgacggt cggcaagaag
      121 ggatacgagg ccgcgtggag cgagctgtcc gaccttgccg gccgggccca ggcggtgaag
      181 gaccgcctcg ttcgggcggt ggacgaggac accgaggcgt tcaacgcggt gcttgccgcg
      241 aaccggatgc cgaagggaac cgccgaagag cgggcggcgc gggacgccgc catcgaggct
      301 ggctaccaga aggcgaccgg ggtgcccctc gaaaccgcga agacgtgcct ggaggcgctg
      361 aagctggcgc aggttgcggc cgcccgcggc aaccgcaact cggcctccga cgccggggtg
      421 gcggccctga tggcccgcgc cgcggtcgag ggcagcgtcc tcaacgtcct gatcaacctc
      481 gggtcggtga aggacgcccg gtttgccgag gcctgccgga cggagaccga gggcctggtg
      541 gccgaggcca acgacctctg cgcacgaacc gtcgccgggg tgaaggggac gttccaggga
      601 tgagggagcg gctcgccgcg ctcgcccgct cgcaacccgg ccggctggcg ttcgcggtgg
      661 gcacccacat cctcacctac ggggagcttg cgcgggacgc cgcgcggctg gcggcggcgc
      721 tgcgaagggc cggcgcggcg ccgggtggcc acgtcgccat cgtgctcccc tccggcctcg
      781 acttcgtccg cgccttctac gcgctcgact ggctgggcgc cgccgccgta cccatcaacc
      841 ccgacttgcc gcccgaacag attcggcgcc ggatcggcgc agcgggctgc cacctcgaga
      901 tcgatgccgc ctcgttcgag gcgttggacg agggctcgaa gagcctgacc ccgctcgatc
      961 ccggaccgcc gtcacccgac tcggtggcct acctgcagct gacgtcgggc acttcggggg
     1021 aacagcgcgc cgcggtgatc ctgcgccgga acctcgaggc gacgctccgc ggcatgcgcg
     1081 acatgatcgg gttcggcccg gaagacgtga tggcgggctc gatccccctg caccacaact
     1141 tcggcctcgt ccggttcgtc tgcgccccgg tctattgcgg ctgcccctgc cacctcaccg
     1201 gggcgccgcc cgcgaacgtc ggccgttggc tcgcgctgct caccgaggtg agggcgaccg
     1261 tgaccgccgc gcccgatttc ctgtatcggg cggcggcgct gcgggtcgac ccctctacgg
     1321 tggaccttcg cgccctccgt ttcgcatcga acggcggcga ggccgttcac ctcgaaacca
     1381 tccggatgtt cgaagagcgc ttcggcgtgc ccggcaccgt gcgcccgggc tacgggcttg
     1441 ccgaaaccac gctcacggtg tcgagcctgg cgccgggctt gccgctgcga tcgatcgacc
     1501 acggcatccc aagctgcggc ccggcgatcc cgggaaccga gatcgccatc gccgacgagg
     1561 acggccggca ccttccccgt ggcgagcgcg gcgagatcgt ggtccgcggg ccatccgtct
     1621 ttgcaggcta ctaccaggat cccgagggca ccgcgcgtgt cttccgcgac ggctggctga
     1681 ggacgggcga ccagggggtg ctcgacgcgg acggcttcct ctacgttacg ggccggcagc
     1741 gctggatgat caagcggggc ggggcgacga tcgtgccggg tgaggtcgag gccgccgccg
     1801 cgtgcgtgcc gggcgtgctg cgagccgcgg cagtcggcgt cgcggccccg cacggcacga
     1861 cggagcgctt cgtggtggcg atcgagatcg acccgcaact gggaagcgac ggcagtctgc
     1921 tgtcggggct cgccggcaag gtggcagcgg cggtgcggca gggcgtcggg caccagcccg
     1981 gcgaaatcgt gctgctcgcc cctggcagcc tgcccctcac cggcagcggc aaggtccgtc
     2041 acggccagct tcgcgacctc ttcgcttcgg gcgaggcgga cgaccgcctc aggcccctcg
     2101 cccgcttctc ataggacggc caccggcggg ccgctggtct ggagccactt cagcaggctc
     2161 agcgacaggt cgtcgggccg cgcgagcagt tggccctcca gccacccagc caccacggcg
     2221 gccatctcgg tttcctgccg gacggcgtcg cccacgcccg cggggcggat tggctctgcg
     2281 gcctctacct caatccgccc cccgtcgccc caccggaagt acaaggggag gatcggagct
     2341 ccgctgagcc gtgccatcac gaacgccccc cgccggaacc gcaccaggcg tccgcagcag
     2401 gcgaccggct cggtggcggc accctcccag ccgtcggccg cgaggacgac cgcgttcccc
     2461 tgccggaggt actcgacgcc gcgccgcagg acctgcgccc ggtgctgatc gtcaccctcg
     2521 agcccaacca cctcgagccc tctcgcgcac gccgtcctgc cgctcttccg gacgatgaga
     2581 gcggtcttcc cgagcaggag cagcgccgcc gatgtggccg agatcgggcc ggtgtggacg
     2641 tcgagcagca cctgcccacc cggctcccgc agccgctgtt cgagccgcgc gaggtggggc
     2701 cgcgagagga gcgatgcggc cgactcgatg ccccccgagt acagcaggcg gtcgtaggcc
     2761 cggcacttca ggtcctgggc ggcaatgcgg cgggccaggc gggcggccgc cgcatcgggc
     2821 agccacggaa acacggccga gacctccgag ggtgccgcgc cccagagacg gccggcgtgg
     2881 cagagccacc cggccgcctc ggcaagccgg acgccggttg ccggcatccg ccgggtgacg
     2941 gcgtcgcacg cacccaccgc cgcccggaac cacgcgggcc gcgcggccat cgggacccgc
     3001 gcgctgacag ggatcttcga gcgcatcagc ggcctgcgcg agcctcgtcc aggtgccgga
     3061 ccagcgtccc gatggtcagt ggatgaacgt cgagcagctg gtcgggaaag gccacgccga
     3121 atcgctcctc gcacgccacc agcagctcca cgatggcaac cgagtcgagg ccaagacccg
     3181 agggtccgat cggcgtgtcg tcgttcacgt tctggatcgt ccaggatggc gccaggcttc
     3241 gcaccagctg gcgcaccaac tccgcctcgc gctctctcgc attgccatct acatccaaca
     3301 caagatttgc catgggagcc tcgccaggtc ggaaaaccgc tcggacatgc taccccagga
     3361 gttttccggc ctgcgcgttg caggtccgac agcagggggt cgagccgatg aagcctgccg
     3421 ttggatttgt cgtcgtgggt tgctgcctgc tcgccgggtt ggccgtcgcg tccccgcccg
     3481 tggccgtgtc ggcgaatccg atggtttcga tggcgcccgc caccgtcgtc gtcaacgtcg
     3541 tgctcgagcc ggacgagcgc aaccgcgcgc tgattgtcgt caccgactcg gccgacttct
     3601 acagctcgag cgaggtagcc ctcgacggcg accgctccgc gcgccagcag cggttcaccg
     3661 tgaggaacct tccggccggg gagtacgtag tccaggcgcg catcaagcgg gcggacaact
     3721 cggagcggat cgccgagacc cacctggtcg tcaccggctt ctgagacggg gtccgccagc
     3781 tccgggtcag ggaagctcga tcgcgtcgag gtacttcggc acggtcgcgg gccacgacag
     3841 ttcctgtcgc agccgtcctg cgaaggtctc ggcgctttcg ggctcgccgt gcacgacgaa
     3901 ggttgccttt ggcggccgct cgacgccgct cagccacctg atcagctcgt cgccgtcggc
     3961 gtgcgccgac atcgagtgca gctgctcgac cgacgcgttg acgggcacca tctggccgtg
     4021 aatcttcacc tcgcgttcgc ctccagccag tcgccgccca cgcgtgccgg cggcctggaa
     4081 gccgacgaag agcacggtat tccgcgggtt gggaagcgcc gacttcaggt ggttgagcac
     4141 ccgcccgccg gtggccatcc cgctcgacga gatgacgatg gaggggatgc gcgaggcaac
     4201 cagctcggcc gactgctgcg gggtcgccac ggtcgtgaaa cgccgggtca cgaacgaggc
     4261 gaactcccct cgaacgccgt gcaggtcggg atccagctcg tccgatcgcc gcgcgtagtg
     4321 cctgagcgcc tcgaccgcca tggggctgtc gacgaagacg gggacgctcg ggatccgccc
     4381 ctcgtcttcg agccgcttca accaccagat gatctcctcg acgcggccga tcgcgaagga
     4441 agggatgatg acctttccgc cgcgatcgat ggtccgcacg acgatctccg ccatcttcac
     4501 cccgtcgtcg tcgggcaggt gggcgcggtc gccgtaggtc gattcgacca gcaggacgtc
     4561 ggccgactcg agcggcgacg ggtcgggcag caccggccgg ttgtagcggc caacgtcgcc
     4621 gctgaagacg acgtgcctgc cgccgtccac gtcgagctcg aggttcgcga aggccgagcc
     4681 gagcaggtgg cccgagttgg taaaggtgac cgtgacgccc ggggcggcgg gcagcgggcg
     4741 atcgaacccc accggctgga ggtgggtgag ggcgcgcacc gcgtcctctt ccgtgaacag
     4801 cggcagcgcc gggctgtgct tggagtagcc gtggcggttc gcctcgcgcg catcttcttc
     4861 ggcgagccgg cccgagtcgg gcaggacgat ccggcacaga tccgccgtgc cgggagtgca
     4921 gaacacccgg ccccgaaacc cctcgcgaac gaggcgggga agatacccgg agtgatcgac
     4981 gtgggcgtgg gtgaggatca ccgagtcgat gctctcggcg gcaaccggaa ggggggccca
     5041 gttccgctcg cgcagttcct tgagcccctg gaagaggccg cagtccacga gcacgcgcga
     5101 ggcgttcact tcgagcaggt acttggagcc ggtgacggtc tgggcggcac caaggaaggt
     5161 cagcgatggc atggctcccc tttcacaccc ccgccaggcc gcgaatccgc gccagcgtgc
     5221 gggccgtgcc cacgagcggg aggttgctga tgtagaagcg ccgcacgccg agggccttga
     5281 gcgcccgcag cgttcgcgcg cacacctctt cggggggcgc tccctcatcg aactcgcgcg
     5341 tgagcccctc ggcgggcacc gagatgaaat ccgcgagcag cgagagcgtc ttcgggttgg
     5401 cgctccggta atagaagacg ccgaagacgc cgggcatcgt gacgccgcga gcgtcggcca
     5461 cctcgagaaa gcgggcgacg ggggtgacct ggtggtgcga gacgacctgc gtcaggaagt
     5521 actcgcccgt gaaccgcgga tcgcagaggt agtccacctg ccgttcggcg ggccggtgag
     5581 ggttcgccca gccgcccagc acgaggtctg accctgtccc tcgaatcgcc tgccgcagct
     5641 cccaggcgtg ctccaccgag cgcgggctcc cgatccgccg gtcgccgccg agcaccacca
     5701 gcgacgggaa cccgtgctgg cgcgcccgat cggcgtacga gaggcagtac tcgagcgggt
     5761 gcttgctcgt gagaatcggc acgatccggt cgcgggcggc gtcgtcgccg atgttcgccg
     5821 tgaggtgacg gaggttgtcc tcctcgggga cgccgacggc gctgtcggtg aggaagacgg
     5881 aggttccctg gagggtcagc gaccgcaccg cgtggtaggt gtcgatccac gcgtccatcc
     5941 cggccgccga cgccagctcc gcacggggcg gccggagttc ggccgcgacg aagggctcct
     6001 ccgatcgcag ggtctcgagc agcgtgctca cgtgaggcta tagaataccg cgcatgacct
     6061 tctccgcggg actcgacgcc ctctgcgcgc tgccgtcgct ctcgctggcg cagtacccct
     6121 cgccaatcga gccgctctcg cgcctgggcg ccgcgctcgg cgccgccccc cgactgctcg
     6181 tcaagcgcga cgacgccctg tcgttcgggt tcggggggaa caaggtccgc aagatcgaga
     6241 tgctggcggc ccgcgccctc gacgacgatg ccgacgtgct gattacgacc gggggggtgc
     6301 agtcgaacca ctgccgggtc accgccgcgg cggcggcgcg gcttggcatg cggtgcgtga
     6361 tcgtcgccaa cggggagagg ccgccgcggg ccaccggcaa tgcgctgctc gacgagctgt
     6421 tcggggcgga cgtgcagtac gtcgcgtcgc gagaggagcg ggcgccggcg atggaggcga
     6481 tcgccgagcg cctccggtca gagggcatgc acccggtgat catcccgctt ggcgcctcca
     6541 ccccgctcgg cgcggccggc ttcgcgcgcg cggtcggcga gctggtgtca cagctggacg
     6601 aggctcccga ctacatcttc cactcgacct cgtccggggg tacgcaggcc ggcctgctgg
     6661 cggggcaggt cctgtacaac gtgaagacgc gcgtgatcgg gatcagcgcc gacgagccgg
     6721 ccgaccgcct gcaggcggcc gtgtcgggga tcgtctccgg gctcggcgag ctgctgggga
     6781 tggacggccg gaaactggcc gcggcccgtc cgatcgaggt cgacacgcgg ttcatcggcg
     6841 agggctacgg tgtcccgacg gaggcctcgc tcgaggccca gtcgctgctg gcccgcaccg
     6901 aggcgctcgt cgtggatcac acgtacaccg caaaggcgct cgcggggctg atcggctggg
     6961 tccgcgaggg gcgattccgc gaatccgaca cggtactctt ctggcacact ggtgggcagg
     7021 tagcggtgtt tgcgtaggtg cgttcacggg cggccgcggg ggccgcgcca accagcacac
     7081 gtgcacaacc ggttgcgcat ccttcacgcc atccacgact tcctgccgcg ccaccaggcg
     7141 ggatcggaga tctacgcctt caacctgtgc cgccagctgg cgcgccgcca tttcgtccac
     7201 gtcctcgccg cggagttcga tccttcccgc cctcacggca cgctgcggtg gcgcgtccac
     7261 gaggggatcc cggtcgtcga gctgatcaac aactgggaga ccgcgtcgtt tgccgacacc
     7321 tggcggtctg ccgtgatcga tcggcggctg ggccaggtac ttcaagcgac gcagcccgac
     7381 gtgctgcacg tgcacagcct gctgaacctg tcgttcaacc tgcccgcgct ggcgcgagcc
     7441 cgcggcatcc cggtcgtcgc gacgctgcac gatcacacgc ttgcctgccc cgccggcggc
     7501 cagcgcctcc atgacaccga gggaacggtg tgcgaccgga tcgacccagc ccgctgcgcc
     7561 ggctgcttcg cccgctcgcc tttccaggcg cagatgtcgg tggcggccgt cggcaggctg
     7621 gcgggccggg ccggtgtgcg cctggtggag gctgcgaggc tggtcgcgcg ccgcatgccg
     7681 cgcgtgaccg cggccctggt gaggagtacc gtcgcgcggc gggtcgctcc ggtctcggcc
     7741 gccgaggtgg gcgagcggct ggccgccctc gctcatgtct tcgaggccgt cgatctcttc
     7801 gtcgcccctt ccgaggccgt ggctcgagcg cacagggagc ttggcctgcc cgatgagaag
     7861 gtggtggtgt cggactacgg cttccctggt ggagcaggac gtcagcccta cgctcaggcg
     7921 cccccacgcc cggtccgcat cgggttcgtg ggcaccctcg tccgccacaa gggcgcgcac
     7981 ctgctgctgg aggcggcaag ggactggccc ggcggccggt tcgagatcga gatccacggc
     8041 agcctcgaca ccttccccgc ctactcgctg cagctgaccc ggtcggccga ggggctcccc
     8101 gtccggttct gcgggccgtt cgatccctcc cgcagcgcct cgatctacgg cgggctcgac
     8161 ctgctcgtcg tcccctccct gtggccggag aactcgccgc tcgtcatcca cgaggcgttc
     8221 ctggcgggcg tgccggtggc tgcggccgcg accggcggaa tcccggaact gatcgccgac
     8281 ggcgtcaacg gggtgctctt cgagcccggg agcgcgcggt cgctcgccga cgcggtgcgg
     8341 cggctgatcg aggacccggc gcggctgcag cgcctcgcct cggcgaagcc ggcggtgaag
     8401 tcgatcgagg cggacgccag ggaatgggag cagcgctacg gcgccctcgt ctcggggagg
     8461 cgcgcgtgaa cgccggccgc agcggcccgt gcgacgcccc gcatccgctg gcgtcggtct
     8521 tgctgctcac cttcaatggc ggtcagaggc tgggcgtcgt gctggaagcg ctcgcgcgcc
     8581 aggaggcacc cttcccgcac gaggttgtcg cggtcgattc cgggtcgacc gacgggacgc
     8641 gggagcggct gcagcaggcg ggggcgagag tgatctccat cccgcgcggc gagttcaacc
     8701 acggggcgac gcgcaacctc ggcatcgagg cctgcaaggg ggtattcgtc gtcctgctgg
     8761 tgcaggacgc cgagccggag ggccgcgact ggctggcgcg gctggtcgat cctctccacg
     8821 aggacgccac gcttgcggga acctatgacc gccaggtgcc gcccgagggg gccggcagcg
     8881 tgccgcgggc gtacctggcc cggcacgccg cggcgtcggc ggaacctcgc gtgcaacagc
     8941 ttgcgggccg ggcggcctac gaggcgctca cgccgtggca gcggatggag gcgtgtacgt
     9001 tcgacaacgt ctgctcctgc gtgcgccggt cggtgtgggc ccgccgcccg ttcgcgcgcg
     9061 caccgattgc cgaggacctg gagtgggcgc gcgacgtcat gctcgacggc taccgcctgg
     9121 cttacgtccc cgaggcgcgc gtgcgccacc tgcacgaccg gcccgcctct tacgagctga
     9181 agcgcacgta cctcgtgcac cagcgcctgc gccgcctctt cgggttgcgc acgatcccct
     9241 cggccgcgca cctggctggc gccgtcctcg tctcgctggc gtcgcacgcg cggtgggttg
     9301 cggccgccgc gggcagcccc tggccgcgca ggcttgccga gctgccgcgc gcgatcgcgc
     9361 tcgccttcgc ccttcctttc gggcagtatc tcggggcgag atcggccgac tcgggccgcg
     9421 atctgcttcg cgtgcggggg gtgtgagtgc gaatcctgtt cgtcgtccac ggcttccctc
     9481 cctccgcgac aggcggaact gaaatctacg ccctcgacct cgccagggcg ctgcagcgtc
     9541 agggccacga ggtccgcgtg ctcgcgcggt tcgccaaccg ggagctgcct gagtacacag
     9601 ccacgcgcca cgttgtgagc ggcgtgcctg tcaccctcgt caacaacacc ttcctgcgcg
     9661 ccgcttcgtt cgaggacacg taccgcaacg ccgccatcga cgcgatcgcc ggcgccgcga
     9721 tcgacgagta cggacccgac gtcgtgcacg tgcaccacct cacctgcctc agcacccgca
     9781 tcgtggcggc ctgccgccag cgcggcatcc cggccgtcat cacgctgcag gactactggc
     9841 ttttgtgcca ccgcggccag ctgctcgatc tcgatctcga gcggtgcgac gggccggccg
     9901 ccgatcggtg ctcgcggtgc gccgggtatg ccgcctcgcc cagccgcgga ctgcacgcgg
     9961 cagctcgcac gctgcgatcc atcgagcggc ggctgccggc aacgctgacc gcagccgagc
    10021 gccggctcgt gtcggcggca tcgcggcggg ccgtgccgcg cggcgcgtcg ggcgccaccg
    10081 cgcggcgact ggaggaggca cgatcggtct gccgcgacgc ggccttgctg ctcgcgccgt
    10141 cacgaaccat cttcgacacg ttccagcggt tcggtgtggg gcgtgagaga atgcgccggc
    10201 aggaccaggg catcgatctc cggcccttcc aggacgtcac gcgcacgcgg ggcgaccggc
    10261 tgcgcctggg attcgcgggg agcctgatgg tctcgaaggc gccccacctt ttcatcgaag
    10321 ccgccgccgg cctgccgcga gaccgggtcg aagtgaccct tgccgggggc cacgccgcct
    10381 atcacggcga cgacagctac gcgagcacca tgcggccgct gctcgaacag ccgtgggtga
    10441 agtggatcgg ccctgtgccg cacgaggaga tgccgcggat cctctccgcg ctcgacgtcc
    10501 tcgtcgtgcc atcgatctgg ctggagaact cgccgttcgt gatccgcgag gcccgggcgg
    10561 ccggcgcggt cgtggtggcg tctcgcctgg gcggcatggc ggaggcggta tcgaacgggc
    10621 gcgacggact gctgttcgag ccggggaacg cctccagcct ccgccgtgcg ctgacgcgcc
    10681 tgctggacga gccggagctg ctggaccgcc ttcgcgaagg gacccgcccg gtcaggtcga
    10741 tcgacgacga cgcggcgtgg accgcggcgg tgtacgagga ggtacgcgac aaggggcgtg
    10801 cttctcccgg cgtgaggcgc gacctcgccg ggaacgcgag cggcagtccc aggattgccg
    10861 ccgtcgtcct caactaccgc gcgcccgacg acacgctgct cgccgtgcgt tcgctcgagg
    10921 cctcgcgccg tcccgttcag ccggtcatcg tcgtggacaa cggcaacgac gggtcgtgct
    10981 cgcgctcgct ggcggcaggc cgttcacggg ctgtgctcat gaagatgcag tcgaaccgtg
    11041 gcttcgcggg cggctgcaac gccggcattt acgagtcgct gcggcgcggg gccgacatgg
    11101 tcgtgctgct gaacagcgac gccgtcgttg cccccgatac cgtggagcgg ctcgaggcgg
    11161 cgctcgcggc cgatcccggc gcgggcattg cggcgccgct cgtcgtctcg cggtccgatc
    11221 ccggcacgat cgcctcggcc ggcatccggt tctccccggt ctccgggagg atgaggcagg
    11281 agggagccgg ggaggcgttc gacggggtgg ccggcgggcc tgcggcgcgc gtgtcggcgg
    11341 tgagcggcgc cgtcatgctg atccggcgcg ccgtcttcga gcaggcgggg ctcttcgacg
    11401 agcgctactt ctactcgttc gaggacatcg agttctgctg gcgcgcggcc cgcgccgggt
    11461 gggcgaccct gctcgtgccg tccgcgctcg cctggcacga ggggcaccgc tcgatcgggc
    11521 cgcagtcggc cgcccgcctc tacttcgcgt cgcgcaacca cctcctgctc nnnnnnnnnn
    11581 gtcgcgccct catcgccccc ggcctcgtgg ctgcgcggcg ccgtcatcgt cctcctcaat
    11641 gccgcccatg ccgcccgcac gcccggcgtc gggatccggc aaggcctgct cgccgtcgcc
    11701 gccggcgccc gcgatcacct ccgcggccgc tacggccgcg cgccgcgcaa gagggcctga
    11761 tacggtcaga tcgtgcaggg ggtgcaaggg gtgcagacgg tgcagtgctg gtggtgctga
    11821 aggtgctgtg caggaggtgc cgaagcctac cgtaccgtag ccgggcactg cgcggcgaag
    11881 gcgggtgtag tgccaggcga gcttccgccg ttcaagccgt tgcatctgtc acgtacgttt
    11941 cgccgagggg ctcagagccg acgagatcac tggaatgatc cgagactctt cgtgcgtctc
    12001 cgtgtctctg tgcctctgtg gtgaacgtgg acaagccgtg acgtctgtta cgtgcggttc
    12061 accgcagagg gcccggcgcc gttatactct cgcgcgtgag ggtgctggtg ctggttcccg
    12121 cccgcgacga ggcgcgcagc ctccccggcc tggtccacgt gctgcgcgca gagcagccgg
    12181 gtgccggcat cctcgtggtg gacgacgcct ccaccgacgc cacgcgccag ctgctgcccg
    12241 ggctgggcgt cgagtggctc accctctgcg cgcacaccgg cgtcgggggc accatccgcg
    12301 ccggcctgcg ctacgcgctg cggcggggct acgacgtcgt ggtgcgggtg gatggggacg
    12361 gccagcaccc ggccgaccag atcgagcggg tgctcgcgcc gattcgggcg ggccaggtgg
    12421 acgccgtcgt cggttcccgt taccggcagg ggtccggcta ccgcgcgcca ggctggcgcc
    12481 gcgccgcgca gcgcgtcctc gcggcctggg tgagcgccgc gatccgccag cgcatcaccg
    12541 accccacctc gggcttctgg gcgttcgggc cgcgggctgt caggctgctc gcgtcacacc
    12601 acccgaccgg ctaccccgag cccgagctgc tgctcctgct cgcccacaac ggcctgcgcg
    12661 tgtcggaggt cccgatcgag atgcgccggc gcctcgccgg acgctcctcg ctgaccgcgg
    12721 cccgcgtcgg cgccatcgcc gggcgcaccg cgctggcgct gctgatcctg cccctgagaa
    12781 ggacggtgcc cgatggcgat gactaacccg atccagctga tagccgtcgc ggtgagcctc
    12841 gcgctcgtcg tggtggtgat cgagctggtg cgccgcgggg cgctcaccga ggagtactcc
    12901 ctcatctggc tcgtctgcgg ggcggcgctg ctcgtgctgt cgttctggaa gaacatcctc
    12961 gacgtgtcgg cgcgctggct cggcatctac tacccgcccg ccgtgctgct gctcgtcgtc
    13021 gtgttcttcg ggttcgtcgc ctcgctctac ttctcggtgg tcatctccgg gcagcggcgc
    13081 cgtatcgagc gcctcatcga ggaggcggcg ctcatcgacg cccgcgtgcg cgaactggag
    13141 gcggaggccg ggacgcgcga ggccgcgtgc cacgccggcc cgtcctaggc gtgaccgcct
    13201 gcggcaggca cgga
//
LOCUS       DAOPQX010000065        13185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5483_length_13185_cov_4.688195, whole genome shotgun sequence.
ACCESSION   DAOPQX010000065 DAOPQX010000000
VERSION     DAOPQX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5483_length_13185_cov_4.688195"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKK95_05365"
     CDS             <1..356
                     /locus_tag="PKK95_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17674.1"
                     /translation="LDFPAIPASVDRIRAGFVADEPPQPLQAALRLTPREAVDGVTLP
                     LDVPVRVACPLCGGRGESWDEWCRGCAGTGDAFFHHRVRLAVPAGVADGARFAFRITA
                     PSAVPTRVEVRVEIR"
     gene            369..767
                     /locus_tag="PKK95_05370"
     CDS             369..767
                     /locus_tag="PKK95_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17675.1"
                     /translation="MRKHVELAGVLYMLWGGICMLLSLSLLSIGVAAAAIGVAAPEDG
                     PGGTMAAGLVAAAFFVLAAAGLVFGGVHAWVGSRLRRFRAHARIPAIVLAVPDLVIVP
                     FGTALGIYALWTLLHDNSKSLFPATRGTGR"
     gene            complement(777..1124)
                     /gene="yidD"
                     /locus_tag="PKK95_05375"
     CDS             complement(777..1124)
                     /gene="yidD"
                     /locus_tag="PKK95_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOC17676.1"
                     /translation="MSRPPGAHRPARRLRPRRLAALVAAAFLGLFVYDVTRPPARQLS
                     ARAAIAGIHAYQRVAAPLLHRAGVRCRFEPNCSRYGEASFERHGFLGGGWRTARRLVR
                     CGPWTPMGTKDEP"
     gene            complement(1141..1500)
                     /locus_tag="PKK95_05380"
     CDS             complement(1141..1500)
                     /locus_tag="PKK95_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17677.1"
                     /translation="MTDHAQRGHPDAHRHVMLVLAYLPPFAVIPMLVEKDDADLQWHA
                     RHGIVLMVAEMLILLGIFAIALVAGLLTAGIGCGLLVIAPVPMLIFLVLHLVIVVRAF
                     AGRRLLIPIVSEYADRF"
     gene            complement(1510..1881)
                     /locus_tag="PKK95_05385"
     CDS             complement(1510..1881)
                     /locus_tag="PKK95_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17678.1"
                     /translation="MTDSTPPAGATSSNRTIMIILSYLWLLALVPLLVEKNDREVQWH
                     AKHGIVLMVAEIVLWVAWTIVSTVVSQIPFLGCLGAAVGALVAFIVWLGIIVLHVLAM
                     VKGINGQRLVVPYVTQYADRF"
     gene            complement(1996..2982)
                     /gene="glk"
                     /locus_tag="PKK95_05390"
     CDS             complement(1996..2982)
                     /gene="glk"
                     /locus_tag="PKK95_05390"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HOC17679.1"
                     /translation="MLLAGDIGATKTLIGLFTPAEERPRLVDVRTVTTLEYDSLEAII
                     ARFLDDHPAAGFISAATFGVAGPIVEQVAKLTNVPWEVDAAAVRDLFGFPSLTLLNDL
                     QAMAYSVPVLEPSELEVLQEGSPAPTGNAALIAAGTGLGEALLHYVDGRFIPSPTEGG
                     HADYAARTPREIGLLQALTGRYGRGEYEQVLSGPGLMNIFRYVHGESPCAAGDPGEDP
                     STGPARVSAAALEGRCPLCVEALDLFVTAYGAEAGNLALHSMASAGVFVGGGIAPKIL
                     PAMRSGAFMEAFRAKGLMTEFLTDVPVAIILNDRAGLLGAAVHANATARSAR"
     gene            3078..4202
                     /locus_tag="PKK95_05395"
     CDS             3078..4202
                     /locus_tag="PKK95_05395"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC17680.1"
                     /translation="MGSPSAKPASTLLAGQHVAFTGRLFSLSRHDARALVARLGGTPV
                     DDVNAKATVLVVGAAGEHRAGAEAEKEKSRKIRRAEAINARQPGRIRVLTEDQFCELA
                     GIPSPAALRQQWYGLRDILAMYPHLREDHLRYLQKWNLIRPALRTHAETYFSFPDLAV
                     LRQANGDLERGAPLRSVLRSLLASREGQLRLDFHLDAQPARVVRLGARTTAAAVSGQA
                     GEIDAALAEELFLVASSLDDGNPLHQEACARAYRRAIEADPNLVPALINLANVHYARG
                     ELVEAETLYQRALTLEDDLFETHFNLGNVYHDLGRLDEAGRCYEAALELNPDYADAHF
                     YLAVTREKQGRSDAARPHWRRYLELAPDGEWVDLAQEFSQ"
     gene            complement(4294..4554)
                     /locus_tag="PKK95_05400"
     CDS             complement(4294..4554)
                     /locus_tag="PKK95_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17681.1"
                     /translation="MSLDGTTTPVEPTSPVAEPTALSAAAQKFQTCRWRRPPEQAIPE
                     GCAHRDVLPITGVNGFDPEAWCPDCAFYKLRRTPKKREYNGY"
     gene            5088..5993
                     /locus_tag="PKK95_05405"
     CDS             5088..5993
                     /locus_tag="PKK95_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC17682.1"
                     /translation="MRIFVTGATGCVGGAVLDALVRGGHQVTGLVRNTERAAQVAARG
                     GAPVIGDLADPGSYRDLAPQHDAVVHAALESSPRGVELERNALEVMLAALSEPGMGPR
                     AFVYTSGVWVIGPTNQPATEETPIKPTAYSVWRPAHEQMVLGAATGDLRTMVVRPGIV
                     YGGSRGIVADLLRDALKGLVRVIGSGENRWAAIYERDLGELYVRLLSRPDASGLYHAT
                     DEADERVNDIVEAIAAAAPQRPDIRRMPLDEARAKLGAYAEALALDQVVRSPRARALG
                     WAPVRRSITGNASRLFEEWRGGRQA"
     gene            complement(5999..6766)
                     /locus_tag="PKK95_05410"
     CDS             complement(5999..6766)
                     /locus_tag="PKK95_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOC17683.1"
                     /translation="MEFNERLVHEVIEAVREQLRKEATDRLRDVYLIGDIEKDSARAV
                     IERLRELAAESAKPITLYINSAGGNVTDGLAIHDVVRHLVWQGIEVTIVVQGMAYSMG
                     SVVLQAASPGARLAFPHSWIMIHEPAKWAGWQSTSAAAQHLDRLKQMQAQIYRILAAR
                     SGKPLGRIIRDTKRTDFYLDAQHAREYGLIDRILVQDALAAESRTEREGGAEEKTAEE
                     APVADDPARVEVAEPAAAEDERRAGKAKRHLDPQAEP"
     gene            6892..7872
                     /locus_tag="PKK95_05415"
     CDS             6892..7872
                     /locus_tag="PKK95_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOC17684.1"
                     /translation="MDDLVSLPDIVAARDRLAGLIRRTPLLRVSPAGGWLGPGGPLLL
                     KCENLQLGGAFKIRGACNMAWRLPEAERKGGLLTYSSGNHGLAIARAAGMLGVPALVV
                     MPTTAPRVKVDGVRALGAEVVFEGTTTLERKARAETEARRRAMTIVPPFDHEWIVAGQ
                     GTIGLEILEQCPAARRVYVQIGGGGMASGIAAAIKQSRPDVEVVGVEPAGAARMTASL
                     AAGRPTTIVVSPGLADGLMAVRPGDITFRHVRAFVDRVVTVDDGAVADAVRWLFEECR
                     LAAEPSGAITVAAAAAARRARPAECEDPEVAVISGGNVDADLFGRLIAGR"
     gene            complement(7880..9271)
                     /locus_tag="PKK95_05420"
     CDS             complement(7880..9271)
                     /locus_tag="PKK95_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17685.1"
                     /translation="MPVSEDRARAVAEAVRQAGGRALIVGGWVRDRLLGIASKDVDVE
                     VYGLPAPRLREVLSAFGPVETVGESFTVYKLGELDVALPRRESKTGRGHRGFTVEGDP
                     SMSVAEAARRRDFTINAIAWDPLTGEYEDPWGGRRDLDRRLLRVVDRHTFPEDSLRVL
                     RAAQFVARFELSVHPATLELCRRIPLDDLPAERVWGEIEKLLLLPERPSLGLAFAREA
                     SVIGRLFPELEALIGCEQEPEWHPEGDVWTHTLLVVDEARRRIGDLPRAGRIAIMLAA
                     VCHDLGKPATTAFKDGRIRSLGHEEAGLAPTTALLDRLNVQSIDGYDVRAQVLGMTAH
                     HLKPGAWYKVRDEVGDGAFRRLAQKVDLELLARLAAADCRGRPGDFDCGAMEWFVGRA
                     RALGVEHEPPRPLLLGRHLLALGLTPGPRIGEILKQVYELQLDGRVTTTEEAIAAARN
                     VLAGREGGGNLAD"
     assembly_gap    9439..9538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9539..9754
                     /locus_tag="PKK95_05425"
     CDS             <9539..9754
                     /locus_tag="PKK95_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17686.1"
                     /translation="VLEVQKVLVLEVQKVLVLGVLVPEVQNVLVPRVPGGLPRRSRAP
                     RDEAGAGRGGDRRISQCADITSPRCCC"
     gene            9718..11916
                     /locus_tag="PKK95_05430"
     CDS             9718..11916
                     /locus_tag="PKK95_05430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC17687.1"
                     /translation="MRRHHFSSLLLLTLALLLGGTAAGQAGTPAATEPAAAAQPARRP
                     ITPQDLWAMKRLGSPALSPDGTRVVFTVQEWSIEKNKSTTNLWLVDVKGGEPRRLTAA
                     EANDGSPAWSPDGTRIAFTSRRDGDQAGALYVIAVGGGEAEKILELPYSVTSPKWLPD
                     GSAVIVGTTVIPELAGTMGRDDVEAIRKEMKRRQESKMTAKVTEDRQYRYFDRYLTDN
                     LAHRLLLVKLADKSFVDLTPRFDRLFQNSGTVSFDVSPDGKTVALTVNSTPRPFRDFP
                     NHDIYLVPTDGSGTMKNVTPENQGDDSSPVFAPDGASLLYNRTKVRYYGGQFPKVWRH
                     DLATGKNTPVTEAFDLAFESVEFSPDGKLLWLTGESEGVVPVFKLDLAAAASAKEGSS
                     FASAAFSAGAAGKALSTVYREGTSTALDVEGGAVVFLNDTTSRPNELFVLDPNGGEPR
                     RLTHFNDGLVAGLELGTVKPYWFTGAAGDRIHGWLVYPPSYDPRKTYPLVQLMHGGPH
                     TMNRDSWSYRWNTHVFAAPGYIVTWVNRHGSTGFGERFSRSIVNQWGDMPFEDIMKAT
                     DHLLRELPNIDPKRMCAAGASYGGYMAAWVLGHTDRFACIVDHAGVNNSYAQYATDVP
                     HGFPEVMGGKPWENLEGMQRQNPMFYAKNFKTPTLVLHGEKDYRVPYGNGLELYGVLQ
                     AMGVPSRLVVFPDENHWVLSPQNAIYWHWEMQNWMGRYLGVKPAIEKPSF"
     gene            complement(12084..13145)
                     /locus_tag="PKK95_05435"
     CDS             complement(12084..13145)
                     /locus_tag="PKK95_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOC17688.1"
                     /translation="MRKRFFLVLGGLVAVAGALAAVNLGYYAGSSYSGSYCTTCHQIQ
                     ASYDKWARSVHRGLQCKECHGSAFTTDLEAHRTNLRHLYYQVSGKVPGRIVLKDAQVD
                     RIAANCQRCHADNLAKWRAGGHSAAYSHIFLSKAHNEKTLLMDDCLRCHGMFADGAIA
                     DIVTPIDNRGPWKLADERFSSRPVIPCLTCHTVHSDGVPPAAPDYLQPKKAAQGRQLR
                     TTSLGFYDRRERRHVLAADLALPAMVVGERAAKMSPDPRQAVCYQCHAPETTLEVGSG
                     DDRTAIGVHEGIGCLGCHDPHGLDARQSCANCHPAYSNCGLDVATMDTSFKSPDSRHN
                     IHFVSCADCHPKGVPERKK"
BASE COUNT         1960 a         4572 c         4611 g         1942 t
ORIGIN      
        1 acctcgactt cccggcgatt cccgcctcgg tcgatcgcat cagggccggc ttcgtcgcgg
       61 acgagccgcc ccaaccgctg caggccgcgc tgcggctgac gccgcgcgag gctgtcgacg
      121 gggtcacctt gccgctcgac gtgcccgtcc gcgtggcctg ccccctttgc ggcggccgcg
      181 gcgagtcgtg ggacgagtgg tgccgcggct gtgccggaac cggcgatgcc ttcttccatc
      241 accgcgttcg cctggcggtc ccggccgggg tcgccgacgg cgcccgcttc gccttccgca
      301 tcacggcccc ctcggccgtt cccacccgtg tcgaagttcg cgtcgaaatc cgctgacgcg
      361 gaccgcccat gaggaagcac gtcgagctgg ccggggtcct ctacatgctg tggggcggga
      421 tctgcatgct gctcagcctg tcgctgctct cgatcggagt ggcggccgcc gccatcggcg
      481 tggcggcgcc cgaggatggc ccgggtggca cgatggcggc agggctcgtc gccgcggcgt
      541 tcttcgtatt ggccgccgcc ggcctcgtgt tcggcggcgt gcacgcctgg gtgggaagcc
      601 gcctccgccg cttccgcgcg cacgcccgca ttccggccat cgtgctcgcg gtccccgacc
      661 tcgtcatcgt gccgttcggc accgccctcg ggatctacgc gctctggacg cttctgcacg
      721 acaacagcaa gagcctgttc ccagcgaccc ggggaacggg acggtaactc ttcccttcac
      781 ggttcgtcct tcgtccccat cggcgtccac gggccgcagc gcacgaggcg ccgtgccgtg
      841 cgccaacccc cgcccaggaa cccgtggcgc tcgaacgacg cctcgccgta ccggctgcag
      901 ttcggctcga agcggcagcg cacgcccgct cgatgcagga gcggcgccgc cacccgctga
      961 taggcgtgaa ttccagcaat cgcggcccgc gcggagagtt ggcgcgcggg cggccgggtc
     1021 acgtcgtaga cgaagaggcc gaggaaggcc gcggcgacca acgccgcgag gcgccggggt
     1081 cgcaggcgcc gggccgggcg gtgggctccg ggcgggcggg acaaaccgag ccagctccgt
     1141 tcagaagcgg tccgcgtact cgctgacgat cgggatcagc agccgccgtc ccgcgaacgc
     1201 gcggacgacg atgaccaggt gcaggaccag gaagatcagc atcggcacag gggcgatgac
     1261 cagcaggccg cagccgatcc cggcggtgag caggccggca acgagcgcga tggcgaagat
     1321 gccgagcagg atcagcatct ccgccaccat cagcacgatc ccgtggcgcg cgtgccactg
     1381 gagatcggcg tcgtcctttt cgacgagcat cgggatcacg gcgaacggcg gcaggtacgc
     1441 cagcaccagc atcacgtgcc ggtgggcgtc cgggtgcccg cgctgggcgt gatccgtcat
     1501 gccccctgcc tagaacctat cggcgtactg cgtcacgtac ggcacgacca ggcgctggcc
     1561 gttgatcccc ttcaccatgg ccagcacgtg cagcacgatg atgcccagcc acacgatgaa
     1621 ggcgacgagg gccccgaccg ccgctcccag gcagccgagg aaggggatct gggaaacgac
     1681 ggtgctcacg atcgtccacg ccacccacag cacgatctcg gccaccatca ggacgattcc
     1741 gtgcttcgcg tgccactgca cctcccggtc gttcttctcg accagcagcg gcaccagcgc
     1801 cagcagccac aggtacgaga ggatgatcat gatcgtccgg ttgctggacg tcgcgccggc
     1861 aggaggcgtg gagtcggtca tagcaatctc tcctcgagta tccgggggct ggtcgtgcgg
     1921 cagtctactg tttccgcagg acactcgtcc agtcgaaagg gccggccggc gagcgcgacc
     1981 ggcgtggccc cgcgatcagc gtgcgctgcg ggcagtggcg ttcgcgtgca ccgcggcgcc
     2041 gagcagcccg gcgcggtcgt tcaggatgat ggcaaccggc acgtcggtca ggaactcggt
     2101 catcagcccc ttcgccctga acgcctccat gaacgccccc gagcgcatcg cgggcaggat
     2161 cttcggcgcg atccctccac cgacgaagac ccccgcgctc gccatcgagt gcagcgccag
     2221 gttgcccgcc tccgccccgt acgcggtgac gaacaggtcg agcgcttcga cgcagagcgg
     2281 gcaccttccc tcgagggcgg ccgccgagac gcgggcgggg ccggtcgagg gatcctcgcc
     2341 gggatcgccg gccgcgcacg gcgactcgcc gtgcacgtac cggaagatat tcatgagccc
     2401 gggccccgag agcacctgct cgtactcgcc gcggccgtac ctgccggtca acgcctgcag
     2461 caacccgatc tcgcgcggag tccgggcggc gtagtcggcg tgccctccct cggtcggcga
     2521 cgggatgaac cggccgtcca cgtagtgcag cagcgcctcc cccaggcccg tccccgcggc
     2581 aatgagggcg gcgtttcccg tcggcgccgg cgatccctcc tgcagcacct cgagctcgga
     2641 cggttcgaga accggcaccg agtacgccat cgcctgcagg tcgttcaata gcgtgaggga
     2701 gggaaacccg aacaggtcgc gcacggccgc cgcgtccact tcccacggga cgttcgtcag
     2761 cttcgccacc tgctcgacga tcgggcccgc gacgccgaag gtcgccgccg agatgaagcc
     2821 cgccgccgga tgatcgtcca gaaaccgcgc gatgatggcc tcgaggctgt cgtactcgag
     2881 ggtggtgacc gtgcgcacgt ccaccagacg gggacgctcc tcggccgggg tgaacagacc
     2941 gatgagcgtc ttggtcgcgc cgatatcgcc tgcgagcagc atggcggcat tctatcaggc
     3001 cgcgcgccgc aaacggcaac agggcgcacg tccccgattg acattcgcgg ccgccttctg
     3061 catcgtggag acgcgccatg ggttccccct ccgcgaagcc ggcctccacg cttctcgccg
     3121 gccagcacgt cgcgttcact gggcggctgt tctccctcag ccggcacgac gcgcgcgcgc
     3181 tcgtggcgcg cctgggcggg acccctgtcg acgacgtcaa cgcgaaggcc accgtgctcg
     3241 tcgtcggcgc ggccggcgag caccgcgccg gtgcggaggc cgagaaggag aagagccgca
     3301 agatccgccg cgccgaggcg atcaacgccc ggcagcccgg ccggatccgc gtgctgaccg
     3361 aagaccagtt ctgcgagctg gccggcatcc cgtcccccgc ggcgcttcgc cagcagtggt
     3421 acggcctgcg cgacatcctc gcgatgtatc cgcacctgcg cgaggatcac ctgcggtacc
     3481 tgcagaagtg gaacctgatc cgcccggcgc ttcgcaccca cgccgagacc tacttctcgt
     3541 tccccgacct ggccgtgctt cgccaggcca acggcgacct cgagcgcggg gcgcccctga
     3601 ggtcagtgct gcgctcgctg cttgcctcgc gcgagggaca gctccgcctc gacttccacc
     3661 tcgatgccca gccggcccgc gtcgtccgcc tcggggctcg aaccacggcc gcggcggtgt
     3721 cgggtcaggc cggcgagatc gacgcggccc tcgccgagga gctgttcctc gtcgcttcct
     3781 cgctcgacga cggcaacccg ctgcatcagg aggcgtgcgc ccgcgcctac cgccgcgcga
     3841 tcgaggccga cccaaacctg gtgcccgcgc tgatcaacct ggcgaatgtg cactacgccc
     3901 gcggcgaact ggtcgaggcc gaaacgctct accagcgggc cctgacgctc gaggacgacc
     3961 tcttcgagac ccacttcaat ctcggcaacg tctaccacga cctcggccgg ctggatgagg
     4021 ccgggcggtg ctacgaggcc gcgctcgagc tgaaccccga ctacgccgac gcccacttct
     4081 acctcgccgt gacgcgcgaa aagcagggcc gttcagacgc ggcacggcca cactggcgcc
     4141 gctacctcga gctggcgcca gacggggagt gggtcgatct cgctcaggaa ttctcgcagt
     4201 aggacgggcg tctcgcctgt cgtaggacgg gcgtctcgcc tgtcgttgga cgggcgtccc
     4261 gcctgtccgt cggaccggtc ggcgccccgc ctatcagtaa ccgttgtact cgcgcttctt
     4321 cggcgtgcgg cgaagcttgt agaacgcgca gtcgggacac cacgcctcgg ggtcgaagcc
     4381 gttgacgccc gtgatgggca ggacgtcgcg gtgagcgcat ccttccggga tggcctgttc
     4441 agggggccgc cgccagcggc aggtctggaa cttctgcgct gcggccgaca gggccgtggg
     4501 ctcggcaact ggtgaggtgg gctcgacggg ggtggtggtc ccgtcgaggg acacgagact
     4561 gtcgaaagcc ggattggtaa ccgccacttt cctccctcgc aagttgtacg cgcaagcaaa
     4621 caaacgctgt ttacaaacat tgtgccacag tgagcgcctc cccgcatact cttgacagat
     4681 cgacggctca gagtgcccga acacctgctc gacctcccgg gccacggcct ccgcgcttct
     4741 cccggccgcg tggacgggcg agccgaacgc cacgcggacc gggccccttc tcggccagcg
     4801 ccatgtgcgg tggagcacgc ggtccacccc ttccagcctc accggacgac cgggacgccc
     4861 agttccccga gtggcgtgtc cggctcgatg tggccgccgc gcgaatgggt ggcgagcagg
     4921 gcttccaggc cgtcgcgggg ctccgccgtc tcccccggct gggctacaat taacggttgg
     4981 cgatcccggc cacgaacaag accgcttcgg gttggccgca ggcatcggca ggatgcagaa
     5041 aacacgtcgg gagcgcgtcg ggcggacccg ctcctcgaga ggaacccatg cgaatcttcg
     5101 tgacgggcgc caccgggtgc gtgggcggcg ccgtgctgga cgcgctggtc cggggcggcc
     5161 accaggtgac gggactcgtt cgcaacaccg agcgggccgc ccaggtggct gcccggggag
     5221 gcgcgccagt gatcggagac ctggccgacc cggggtcgta ccgggacctc gccccgcagc
     5281 acgacgccgt cgtccacgcc gcgctcgaat cgagcccgcg tggcgtcgag ctcgagcgca
     5341 acgctctcga ggtcatgctg gccgcgctca gcgagccggg catgggtccg agggccttcg
     5401 tgtacacctc gggggtctgg gtgatcggtc ccaccaacca gcccgccacc gaggagacac
     5461 cgatcaagcc gaccgcctac tcggtatggc gcccggctca cgagcagatg gtcctcggcg
     5521 ccgcaaccgg cgacctccgc accatggtgg ttcggcccgg gatcgtctac ggcggctcgc
     5581 gcggcatcgt cgccgacctg ctgcgggatg cgttgaaggg cctggtgcgt gtcatcggca
     5641 gcggtgagaa ccggtgggcg gcgatctacg agcgcgacct cggcgagttg tacgtccggt
     5701 tgctgtcgcg gcccgacgcc tcgggcctct accacgccac cgacgaggcc gacgagcgcg
     5761 tcaacgacat tgtcgaggcg atcgccgcgg cggcgccgca gaggccagac atccggcgca
     5821 tgccgctcga cgaggcccgc gccaagctgg gtgcgtacgc cgaggcgctt gccctcgacc
     5881 aggtggttcg cagcccgagg gcccgggcgc tgggctgggc gcccgtccgc cgctcgatca
     5941 cgggcaacgc ctcccgcctg ttcgaggagt ggcgcggcgg ccgccaggcg tagaagtctc
     6001 agggctcggc ttgcggatcg aggtgccgct tcgccttccc ggcacggcgt tcgtcctcgg
     6061 cggcggcggg ctcggcgact tctacacgcg ccgggtcgtc ggcaaccggc gcctcctcgg
     6121 cggtcttctc ctccgcgccg ccttcccgct cggtccgtga ttcggccgcc agcgcgtcct
     6181 gaaccaggat ccggtcgatc aacccgtact ccctggcgtg ctgggcatcg agatagaagt
     6241 cggtgcgctt ggtgtcgcgg atgatgcgtc caagcggctt gcccgagcgg gccgcgagga
     6301 tccggtagat ctgcgcctgc atctgcttga gacggtccag gtgctgcgcc gccgccgagg
     6361 tggattgcca gccggcccac ttggccggct cgtggatcat gatccacgag tggggaaacg
     6421 ccagccgcgc gcccgggctg gcggcctgca gcacgacgga tcccatcgag taggccatcc
     6481 cttggacgac gatggtcacc tcgatgccct gccagacgag gtgacgcacc acgtcgtgga
     6541 tggcgagccc atccgtcacg ttgccgcccg cgctgttgat gtacagggtg atcggcttgg
     6601 cactctcggc ggccagctcg cggagccgct cgatgacggc gcgggcgctg tccttctcga
     6661 tgtcgccgat gaggtagacg tcgcggagcc tgtcggtggc ttccttccgg agctgttccc
     6721 ggacggcttc gatgacctcg tgcaccaggc gctcattgaa ctccatcgtg tgtcctggac
     6781 ggccagccgc ctcgaccggc gacggcggga gtaaggagat gccggccaca cgccatcaac
     6841 aatcgtgccg cgtcgtcgcc gcgagggcgg ggccggtggt agcatctcgg cgtggacgat
     6901 cttgtttctc ttcccgacat cgtcgcggcc cgcgaccgcc ttgcggggtt gatccggcgc
     6961 acgccgctgc tgagggtcag ccccgctgga ggctggttgg gcccgggcgg tcccctgctg
     7021 ctgaagtgtg aaaaccttca gctcggcggc gcgttcaaga tccgcggcgc gtgcaacatg
     7081 gcctggcgcc tgcccgaagc cgagcggaag ggcggcctgc tgacgtactc ctcgggcaat
     7141 cacgggctgg cgatcgcgcg cgcggcagga atgctgggcg tgccggcgct cgtggtcatg
     7201 ccgacgaccg cgccgcgcgt gaaggtggac ggcgtacggg cgctgggggc ggaggtcgtc
     7261 ttcgagggca cgacgacgct cgagcggaaa gcgagggccg agaccgaggc gcgccggcgt
     7321 gcgatgacga tcgtgccgcc attcgatcac gagtggatcg tggcggggca ggggacgatc
     7381 gggctcgaga tcctggagca gtgccctgcc gcgcggcgag tgtacgtgca gataggcggc
     7441 ggcggcatgg cctcgggcat cgccgcggcg atcaagcaga gccggcccga cgtggaagtg
     7501 gtcggtgtcg aaccggcggg cgcggcgcgc atgacggcct cgctggcggc gggccggccc
     7561 accaccatcg tcgtgagtcc cggactggcc gacgggctga tggccgtgcg gcccggcgac
     7621 atcaccttcc gccacgtgcg ggcgttcgtc gatcgggtcg tcaccgtcga cgacggggcc
     7681 gttgccgacg cggtgcgctg gctgttcgag gagtgccggc tggcggctga accgagcggt
     7741 gcgatcacgg tggccgccgc agccgcggca cgccgagcgc ggccggccga atgcgaggac
     7801 cccgaggtgg cagtcatcag cggcggcaac gtcgatgcgg acttgttcgg caggctgatc
     7861 gccggccgct gagggctcgc tagtccgcga ggttgccgcc gccttcccgg cccgccagaa
     7921 cgttcctggc ggcggcgatc gcctcctcgg tcgtggtgac ccggccatcg agctgcagct
     7981 cgtacacctg cttcaggatc tcgccgatcc tggggccggg tgtcaggccg agcgccagca
     8041 ggtggcggcc gaggagcagc ggcctgggcg gctcgtgctc gacgccaagc gcccgggcgc
     8101 ggccgacgaa ccactccatc gcgccgcagt cgaagtcgcc cggccggccg cggcagtctg
     8161 ccgccgcaag gcgggcgagc agctcgaggt ctaccttttg cgccaggcgc cggaaggcgc
     8221 cgtcgcccac ctcgtccctc actttgtacc aggctcccgg tttcaggtgg tgcgcggtca
     8281 tgccgagcac ctgcgcccgc acgtcgtagc cgtcgatcga ctgcacgttc aagcggtcga
     8341 gcagcgcggt cgttggggcc aggccggcct cctcgtggcc gagcgagcgg atgcgtccgt
     8401 ccttgaacgc ggtggtggcc ggcttgccga gatcgtggca gaccgcggcc agcatgatcg
     8461 caatgcggcc ggcacgcggg agatcgccga tgcgccggcg cgcctcgtcc acgaccagga
     8521 gggtgtgagt ccagacgtct ccctcagggt gccactcggg ttcctgctcg cagccgatga
     8581 gcgcctccaa ctccgggaac aaccggccga tgacactggc ctcccgggcg aacgccaacc
     8641 cgagcgaagg gcgctcgggc aggaggagca gcttctcgat ctcgccccac acgcgctcgg
     8701 cgggcaggtc gtcgagcggg atccggcggc acagctcgag cgtcgccggg tggaccgaca
     8761 actcgaagcg ggccacgaac tgcgccgccc ggagtacgcg caggctgtcc tcgggaaacg
     8821 tgtggcgatc gaccacgcgc agcaggcggc ggtcgaggtc gcgacggccg ccccaggggt
     8881 cctcgtactc gccggtcaac gggtcccagg caatcgcgtt gatcgtgaaa tcgcggcggc
     8941 gcgcggcctc ggccacgctc atcgacggat cgccctcgac ggtgaagccg cggtggccgc
     9001 ggccggtctt cgactcgcgg cggggaagcg ccacgtccag ttcgccgagc ttgtagaccg
     9061 tgaagctctc accgacggtc tcgacggggc cgaaggcgga gaggacttcg cgaagccgcg
     9121 gcgcggggag gccatacacc tcgacgtcca cgtccttgga cgcgatcccg agcaggcggt
     9181 cgcgcaccca gccgccgacg atcagtgcgc ggccgccggc ctggcgcacc gcttcggcca
     9241 ccgcacgcgc acggtcctcg gagacgggca tggggggatc gtagcatctc gtcgtgctgg
     9301 aggtgctgga ggtgctggag gtgcagaagg tgctggtgct ggaggtgcag aaggtgctcg
     9361 tgctggaggt gcagaaggtg ctcgtgctgg aggtgcagaa ggtgctggtg ctggaggtgc
     9421 agaaggtgct ggtgctggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     9541 gctggaggtg cagaaggtgc tggtgctgga ggtgcagaag gtgctggtgc tgggggtgct
     9601 ggtgccggag gtgcagaacg tgctggtgcc gagggtgccg gggggcctgc cgcgccgtag
     9661 ccgcgcaccg cgcgacgagg cgggtgcagg gcggggcggg gatcgtagaa tctctcaatg
     9721 cgccgacatc acttctcctc gctgctgctg ctgacgctcg ccctcctgct tggcggcacc
     9781 gctgccgggc aggcgggaac gcccgccgcc actgagccgg ccgccgccgc gcagccggcc
     9841 aggcgcccga tcacgccgca agacctctgg gcgatgaagc gcctgggcag cccggcgctc
     9901 tcgcccgacg gcacccgcgt cgtcttcacc gtgcaggagt ggtcgatcga gaagaacaag
     9961 agcacgacca acctgtggct ggtggacgtc aagggcggcg agccgcggcg gctcaccgcc
    10021 gccgaggcca acgacggctc gcccgcctgg agccccgacg gcacgcggat cgcctttacc
    10081 tcgcgccgcg acggcgacca ggccggcgcg ctctacgtca tcgcggtcgg cggcggcgag
    10141 gccgagaaga tcctcgagct gccctacagc gtcacctcgc ccaagtggct ccccgacggc
    10201 tcggccgtca tcgtcggcac caccgtgatc ccggagctgg ccggcacgat gggcagagac
    10261 gacgtcgagg cgatccggaa ggagatgaag cgccgccagg aatcgaagat gacggcgaag
    10321 gtcaccgagg atcggcagta ccgctacttc gaccgctacc tcaccgacaa cctggcgcac
    10381 cggctgctgc tggtgaagct cgcggacaag tcgttcgtgg acctcacgcc ccgcttcgac
    10441 cgcctgttcc agaacagcgg cacggtgagc ttcgacgtct cgcccgacgg caagacggtg
    10501 gcgctcacgg tgaactcgac gccgcggccg ttccgcgact tcccgaacca cgacatctac
    10561 ctcgtgccga ccgacggctc ggggacgatg aagaacgtga cgcccgagaa ccagggcgac
    10621 gacagcagcc cggtgttcgc gcccgacggc gcctcgctcc tctacaaccg gacgaaggtt
    10681 cgctactacg gcggccagtt cccgaaggtg tggcggcacg acctggccac cgggaagaac
    10741 accccggtca ccgaggcgtt cgacctcgcg ttcgagagcg tggagttctc gcccgacggg
    10801 aagctgctgt ggctcaccgg cgagagcgag ggggtggtgc cggtgttcaa gctcgacctg
    10861 gccgcggccg cgtccgccaa ggaaggctcg tcgttcgcca gcgccgcctt ctccgccggc
    10921 gccgccggca aggcgctctc caccgtctac cgtgaaggga cctcgaccgc gctcgacgtc
    10981 gagggcgggg ccgtcgtgtt cctgaacgac accaccagcc ggccgaacga gctgttcgtg
    11041 ctcgacccga acggcggcga gccccggcgc ctcacgcact tcaacgacgg gctggtggcc
    11101 gggctcgagc tgggcacggt caagccctac tggttcaccg gtgcggcggg cgaccggatt
    11161 cacgggtggc tcgtctaccc gccgtcgtac gacccgcgca agacctaccc gctggtgcag
    11221 ctgatgcacg gcggcccgca cacgatgaac cgcgacagct ggagctatcg ctggaacacg
    11281 cacgtcttcg cggcgcccgg ctacatcgtc acctgggtga accgccacgg gtcgaccggc
    11341 ttcggcgagc gcttctcgcg cagcatcgtc aaccagtggg gcgacatgcc gttcgaggac
    11401 atcatgaagg cgaccgacca cctgctgcgc gagctgccga acatcgaccc gaagcggatg
    11461 tgcgcggccg gcgccagcta cggcggctac atggcggcgt gggtgctggg ccacaccgac
    11521 cggttcgcct gcatcgtcga tcacgcgggc gtgaacaact cgtacgcgca gtacgcgacc
    11581 gacgtcccgc acggcttccc cgaggtgatg gggggcaagc cgtgggagaa cctcgagggg
    11641 atgcagcgcc agaacccgat gttctacgcg aagaacttca agacgccgac gctcgtcctg
    11701 cacggcgaga aggactacag ggtgccgtac ggcaacggcc tcgagctgta cggcgtgctg
    11761 caggcgatgg gcgtgccgtc acggctggtc gtgttccccg acgagaacca ctgggtgctc
    11821 tcgccgcaga acgcgatcta ctggcactgg gaaatgcaga actggatggg gcggtacctt
    11881 ggcgtgaagc cggcgatcga aaagcccagt ttctgacgat cgcgtgtcca cgttcaccac
    11941 agaggcgcaa agacacagag gcctgtcacg aatttgatct cacactgtcg cgctgcatcg
    12001 cgtgtccacg ttcaacgcag aggcgcggag gcgcagaggg cttgtcacga tccacgaatc
    12061 ctgaatctgc agcgacagcg tcgtcacttc tttcgttccg gcactccctt cgggtggcag
    12121 tcggcgcatg acacgaagtg gatgttgtgg cggctgtccg gcgacttgaa gctcgtgtcc
    12181 atcgtcgcca cgtcgaggcc gcagttcgag taggcggggt ggcagttcgc gcacgactgc
    12241 cgcgcgtcga ggccgtgcgg gtcgtggcag ccgaggcagc cgatcccctc gtgcaccccg
    12301 atcgcggtgc ggtcgtcgcc cgacccgacc tcgagggtcg tctccggcgc gtggcactgg
    12361 tagcagacgg cctgccgcgg gtcgggactc atcttcgcgg cgcgctcccc gacaaccatg
    12421 gccggcagcg cgaggtcggc ggccagcacg tgccgcctct cgcgccgatc gtagaagccg
    12481 agggaggtcg tccgcagctg gcgcccttgc gcggccttct tcggctgcag gtaatcgggc
    12541 gccgcgggcg ggacgccgtc actgtgcacg gtgtggcagg tgaggcacgg gatcacgggg
    12601 cggctggaga accgctcgtc ggcgagcttc cacggcccgc ggttgtcgat cggcgtgacg
    12661 atgtcggcaa tcgcgccatc ggcgaacatg ccgtggcacc gcaggcagtc gtccatcagc
    12721 agcgtcttct cgttgtgcgc cttcgacagg aagatgtgcg agtaggcggc cgagtggccg
    12781 cccgcccgcc acttcgcgag gttgtcggcg tggcaccgct ggcagttggc ggcgatgcgg
    12841 tccacctgcg cgtccttcag gacgatgcgt cccggcacct tcccgctcac ctggtagtag
    12901 aggtgacgca ggttcgtccg gtgcgcctcg aggtcggtgg tgaaggcgct cccgtggcac
    12961 tccttgcact gcaggccgcg gtgcaccgag cgcgcccact tgtcgtagga cgcctggatc
    13021 tggtggcacg tcgtgcagta gctgcccgag tagctcgacc ccgcgtagta accgaggtta
    13081 accgcggcaa gcgcgccggc gacggctacc aacccgccca ggacgaggaa gaaacgtttg
    13141 cgcatggtgt gcagccccga gccccgagcc cctagcccct agccc
//
LOCUS       DAOPQX010000066        12943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5618_length_12943_cov_5.093032, whole genome shotgun sequence.
ACCESSION   DAOPQX010000066 DAOPQX010000000
VERSION     DAOPQX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5618_length_12943_cov_5.093032"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PKK95_05440"
     CDS             complement(<1..908)
                     /locus_tag="PKK95_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOC17689.1"
                     /translation="MGWIGRTTVIVAMVAAAGLTAGAAVQSTAKDKEKAAPQKDKVRI
                     IERVGPGGRVFAAIGGGARLGVTLQEVTGEKLQGPGGARVNEVDEDSPAAKAGLKSGD
                     VIVEFDAERVRGVRQLQRLVSETPAGRPVKLAVMRDGKRVELSATLEERDWPDVIGLD
                     REKLRGEIEPLVREGMRGMREFRWKTPEGQGPGDLERKEPGPRWNFRVEPGWPMFEWS
                     PGGASRLGVAVQEMSDQLRGHFGADAGVLVASVNPESAASRAGIKAGDVITTVAGKKI
                     ETTGDLVQAIRAADDDAEIEIGIVRDR"
     gene            1236..1604
                     /locus_tag="PKK95_05445"
     CDS             1236..1604
                     /locus_tag="PKK95_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17690.1"
                     /translation="MRYLATRTIAAAVLLSLVAGGCALAVRRPSVAELHYNSGRYYDK
                     RVAIEGEVTSAWGLPLVPFSMYKVSDGTGEVTVLSQGSRLPSRGARVRVTGRVSELAV
                     LGGRPLGLHIREHDLDYRRW"
     gene            complement(1620..3413)
                     /locus_tag="PKK95_05450"
     CDS             complement(1620..3413)
                     /locus_tag="PKK95_05450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOC17691.1"
                     /translation="MTHPSSEVPAGHPSECQRPSDQQLLTTLFDLGRQVTSVLDLDEL
                     LQRIPQLISRLTDYQAFAVYLIDEKRGDLSIAYAVGYPPEALQGLRLKIGEGIIGAAV
                     RDERPILVDDVSADPRYTEIVPGTRSELVVPLRRKGKVIGALNLLSPNVGQFNELDEK
                     ILRQFAATLASGIENARLFEREREYNEMLETLAEIGREVSSILDLDELLTRLAQLAKR
                     VIDYRTFGILLLNEDTGELEMKLALKYGEQTTVPPVRLGEGLVGHAALHKRTVLVPDV
                     TQDSRYVKVLDDVRSELVIPLLVKDRCIGVFDIESPELDAFGKTHVEVMTLLASQAAV
                     AIENARLYETVRANEERLEKELRFAQRVQAALLPQGLPRRLKGVDAAARFAPARELGG
                     DLYDFLAPDGHTLIVAVGDVSGKGVPAALYSAFAGELIRSRTFRRRFDAGGFVAAGVL
                     SSINTILHERHLEEYYCTLCYAVFDMKRRRVVIANSGLPYPLRCAGDGCHPVEVAGIP
                     LGAFPGSTYDEVTFDLKAGDLFVFCSDGVFEAMDSEGREFGSARLLQTLQAARRGTAE
                     EIVDAIFTAVQDHRGDEPPNDDTTAVAIRIT"
     gene            3440..3898
                     /gene="fabZ"
                     /locus_tag="PKK95_05455"
     CDS             3440..3898
                     /gene="fabZ"
                     /locus_tag="PKK95_05455"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOC17692.1"
                     /translation="MDLSLPLDHPAIERILPHRFPFLLVDRITEFEPDKRVVGVKNVT
                     RDDHLLTEEPGGEAVLPPTVLTEAVAQVGAILILAKPENREKLIFFGGMESVRYKRPV
                     RAGDVVVVEASVRRLRSRMGLLKGVAKVDGKVCIVGTMTFALGDSSKDKA"
     gene            4033..4182
                     /locus_tag="PKK95_05460"
     CDS             4033..4182
                     /locus_tag="PKK95_05460"
                     /inference="COORDINATES: protein motif:HMM:NF039824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HOC17693.1"
                     /translation="MLETIAIILIVLWLLGMVSGYTMGNFIYILLVIAIVLFLVRLVS
                     GRRVV"
     gene            complement(4160..5134)
                     /locus_tag="PKK95_05465"
     CDS             complement(4160..5134)
                     /locus_tag="PKK95_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOC17694.1"
                     /translation="MMATLPRIKQLTAESSGVGFFLCVQKDVRQGRAGDFVSLALQDA
                     TGRIAAKIFDEVERLKGEFDAGEFVKVRARGNLYNDRLQLIVENIRRVHPDQDRKDGF
                     REEDCVLSAPRPLDEMWAELQAVVHGVSDGWIRALLEKIACENEERLRVWPAAQTLHH
                     AYRGGFLEHVLKIAEVVSSLAALYGANRDVAVAGALLHDIGKLYELDYGPATSYSREG
                     RLVGHIAVGVRLVREAASAIDGFPPALLTELEHLVLSHHGCLEFGSPVVPMTIEAFIL
                     SFADDLDAKINMARQALGDDTSDGEFTAWHGRLERVLWKGAVRPPASR"
     gene            5271..7556
                     /locus_tag="PKK95_05470"
     CDS             5271..7556
                     /locus_tag="PKK95_05470"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOC17695.1"
                     /translation="MTEGTVVETAVDRAEKVSLPVTGMTCAACARTIQVTLEDTRGVR
                     QADVNFATGRATVVFDPSAVNLDGLVGAIRDVGYDVLDEPDTGDEEAVADAQEQAHRA
                     EYRAARRKFLVAVVFALPIMVIGMAHLQFPGVNWVQLALALPVVFYSGAQFYRGAWAA
                     VRHRNADMNTLIAVGTGVAFAYSVIVTVAPGTVMHPVHEGVMSHAPGVYYEVATAIIA
                     LVLLGRMLEARARGRTSEAIKRLIHLQPRTALVERGGVEVEIPAREVRRGDVVIVRPG
                     ERLPVDGEVIEGASAVDESMLTGESLPVDKAPGSAVYGGSVNSTGGFRFRATRVGRET
                     TLQQIIRLVQEAQASRAPIARLADVISGYFTPIVIAIAILTFVIWFDVMPAGNRFTPA
                     LVSFVAVMIIACPCAMGLATPTAILVGTGRGAEKGILIRGGEILERAGHITTVVLDKT
                     GTITSGRPEVTDLVPAPGIVPDDLLGLAASAERASEHPLAQAIVRAAGERGIPLRPVS
                     EFRALPGLGISAATGEGRLVIGNQGLMDREGIDTSPLAAEMDRLAQQARTVMWAGMNG
                     RLVGLVGMADTPRPESAGALRRMKAMGLELVMITGDNAATANAIAGQVAPGGGIDRVV
                     AGVLPERKAQEVKALQAEGRVVAMVGDGINDAPALAQADVGIAVGSGTDVAIEAADIT
                     LMRNDLNGVAEAIHLSRRTLRVIKQNLFWAFIYNVLGIPIAAGALYPFTGWLLNPMLA
                     AAAMSFSSVSVLANSLRLRRG"
     gene            7569..7727
                     /locus_tag="PKK95_05475"
     CDS             7569..7727
                     /locus_tag="PKK95_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HOC17696.1"
                     /translation="MLDNTQIVVSVLGLGLIAGVFWFFFGGKRRRPPSPDARLARGYG
                     GKPQSREP"
     gene            7801..9303
                     /locus_tag="PKK95_05480"
     CDS             7801..9303
                     /locus_tag="PKK95_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC17697.1"
                     /translation="MAARLFDRAQVGACVAVLGPDGADRTLVVNTHLKHIFGYESSVP
                     ESQVLPFESARFVDPLARSNLLERLGHDGAVADFLVRVRRVDFTPIWVEVTATAERQD
                     GGSLLVEALVRDVSDRKKLDDQTRDLYGQLLQAEKLASLGQTISGIAHELNNPLATVL
                     TWAERLAPRQKDADTRRGLEVILSEADRAARIVRNLLTFARKRHTTRTMVDLNQVVGE
                     TLALRAYEQRVTNINVISALAAGLPQVFADPHQLQQVLLNLVINAEQAMLAANGRGTL
                     VVRTWHDASTDTVVLEVNDDGPGIAEELQAKVFDPFFTTKEVGKGTGLGLTVAYAIVK
                     EHGGRIWVRGSQGQGASFFVELPTGEAQVVRAPQPPPEQIRAEVPAGTRVLVVEDEAA
                     LAAAVTEGLSDAGFEVDHASDGEEALAHVAARAYDLIVCDLKMPRVDGMEFYRAIATG
                     NPALARRVIFCTGDVVGAEAERFLAESGCRWLAKPFRLADLLRAVRELLA"
     gene            complement(9492..10178)
                     /locus_tag="PKK95_05485"
                     /pseudo
     CDS             complement(9492..10178)
                     /locus_tag="PKK95_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
     gene            complement(10605..10865)
                     /locus_tag="PKK95_05490"
     CDS             complement(10605..10865)
                     /locus_tag="PKK95_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17698.1"
                     /translation="MNPSLQKPTPLAHMPADDLGDLGALFHRLNNELGIILSHAELLE
                     AKAGDEISRSRASQVVTSVLDAMGTARSIRSRVSDIQSRFQV"
     gene            11013..12401
                     /locus_tag="PKK95_05495"
     CDS             11013..12401
                     /locus_tag="PKK95_05495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC17699.1"
                     /translation="MSENTKHLLYVEDEVALRQVVAERLTERGYEVVEVSNGEEALER
                     LAEFAFDIVITDLRLPGIDGARVIEQAIASYPDIIGIIVTGFGTVKDAVDAIKRGATD
                     FITKPFQFEELMLVLDAALEQRRLRSENAYLRSQLEQRYRFEGIIGRSRPMRNLFQLL
                     ETVAPTNSTILVTGETGTGKELVARAIHHNSPRRASRFVALNCSAIPETLLEAELFGH
                     VRGAFTGAVANRPGRFEQAHKGTLFLDEVGTMSIALQMKLLRAVQEREFEKVGDSRPT
                     KVDVRVIAATNSDLAQMVRDGTFREDLYYRLNVIPIHLPPLRERREDIPLLVQHFLDR
                     FCRELVPSRPALTVSQHAMRRLMGFSWPGNVRQLENALERAVALSGGRDQIEVADLPP
                     DVQEVGGGSMPQEFALPEEGIDFEEYVSKVELDLIKQSLERTGGNKGRAAQLLNLKRT
                     TLVEKLKRLNES"
     gene            12419..>12943
                     /locus_tag="PKK95_05500"
     CDS             12419..>12943
                     /locus_tag="PKK95_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17700.1"
                     /translation="MPPRFAFWTIVIDGSPTAFRAAKRDDLLPTLKQLQGRNPHAELK
                     WFARGKVWESPEQARASRERGGPPRRDSFAHGGAGGAGDERRGKQWRPGGEHRDPREK
                     FKKETYQARKRREKKAAALARQGGAGFPPPGGKTHGGRDRREPQDRPWKPNRPREGRP
                     PQEQPWRPQRPREGS"
BASE COUNT         1948 a         4533 c         4520 g         1942 t
ORIGIN      
        1 cggtcgcgga cgatcccgat ctcgatctcc gcgtcatcat ccgcggcgcg gatcgcctgc
       61 accaggtcgc ccgtcgtctc gatcttcttg ccggcgacgg tggtgatcac gtcgcccgcc
      121 ttgatgcccg cgcgcgaggc ggcagactcg gggttgacgc tcgccaccag gacgcccgcg
      181 tccgcgccga agtggccgcg cagctggtcg ctcatctcct ggacggcgac gcccagcctg
      241 ctcgcccccc cgggtgacca ctcgaacatc ggccaccccg gctccacccg gaagttccag
      301 cgggggccgg gttccttccg ctcgaggtcg ccgggcccct gcccctcggg ggtcttccac
      361 cggaactcgc gcattccgcg catcccttcc cgcacgagcg gctcgatctc accccgcagc
      421 ttctcgcggt cgagcccgat cacgtcgggc cagtcgcgct cctcgagcgt ggccgacagc
      481 tccacccgct tgccgtcgcg catcacggcg agcttcaccg gccgccccgc cggcgtctcg
      541 gacacgaggc gctgcagctg gcggacgccg cgcacccgtt cggcgtcgaa ctcgacgatg
      601 acgtcgcccg acttcaggcc ggccttcgcc gccgggctgt cctcgtcgac ctcgttcacc
      661 cgcgcgccgc ccggcccctg cagcttctcg cccgtcacct cctggagagt gacgccgagc
      721 cgcgccccgc cgccaatggc cgcgaagaca cgcccgccgg gaccgactcg ctcgatgatc
      781 cgaaccttgt ccttctgcgg cgcggccttc tccttgtcct tggcggtgga ctggaccgct
      841 gcgcccgccg tcaaccctgc cgccgccacc atcgcgacga tgaccgtcgt cctgccaatc
      901 caacccatgt actgcctccc gtttcagcgg ccctggccac cgactctgtc agttcagacg
      961 agtgagccgg gggattggtc gcttcgcctc ccgctggctt tcgtcctcgg cgagctacgg
     1021 cgcgactgtc accgggggtg agcggcggcc gcggcgaggc gccgtttttc actcgaaaat
     1081 cgcgcgcgcg ccgccggcac gagctttgat gcctgtcatg gcaagcgcgc ggggccggcg
     1141 ggatcgttca ccggcaggcg tcggcacgcc agtcccgcat cgggttcggt cccgcgcgcg
     1201 cgtatgggcc ccaccccgct catcgggagg ctgacatgcg atacctcgcc actcgcacca
     1261 tcgctgccgc cgtgctgctt tccctggtgg cgggaggctg cgcgcttgcc gtccgccggc
     1321 cgagcgtcgc cgagctgcac tacaacagcg gccggtacta cgacaagcgg gtcgcgatcg
     1381 agggcgaggt gacgagcgcg tggggcctgc cgctggtgcc cttctcgatg tacaaggtga
     1441 gtgacgggac cggcgaggtg acggtgctct cccaggggtc gcgcctccct tcgagaggcg
     1501 cgcgcgtgcg ggtgaccggc agggtgagcg agctggccgt gctcggcggc cgcccgctcg
     1561 gcctccacat ccgcgagcac gacctcgatt acagacgctg gtgaaccagg cccgagcccc
     1621 taggtgatcc tgatcgcgac cgccgtcgtg tcgtcgttcg gcggctcgtc gccccggtgg
     1681 tcctgcaccg cggtgaagat cgcgtccacg atctcctcgg ccgttccccg ccgcgccgcc
     1741 tgcagcgtct gcagcagccg cgccgaaccg aactcgcgcc cctcgctgtc catcgcctcg
     1801 aacacgccgt cgctgcagaa cacgaacagg tcgccggcct tcaggtcgaa ggtcacctcg
     1861 tcgtaggtgg agccggggaa ggcgccgagg ggaatcccgg cgacctccac cgggtggcac
     1921 ccgtcacccg cgcaccgcag ggggtagggc aggccggagt tggcgatcac gacgcgccgc
     1981 cgcttcatgt cgaagacggc gtagcagagc gtgcagtagt actcctccag gtggcgctcg
     2041 tggaggatgg tgttgatcga cgagaggacg ccggccgcca cgaagccccc ggcgtcgaag
     2101 cggcggcgga aggtgcgcga ccggatcagc tcgccggcga acgcgctgta cagcgccgcg
     2161 ggcaccccct tgcccgacac gtcgcccacc gcgacgatga gcgtgtggcc gtcgggcgcg
     2221 aggaagtcgt agaggtcgcc ccccagttcg cgggccggcg cgaagcgggc ggcggcatcg
     2281 acgcccttca ggcggcgggg cagcccctgc ggcagcagcg ccgcctggac gcgctgggcg
     2341 aaccgcagct ccttctcgag ccgctcctcg ttggcgcgca ccgtctcgta cagccgcgcg
     2401 ttctcgatcg caaccgccgc ctggctggcg agcagcgtca tcacctcgac gtgggtcttg
     2461 ccgaaggcgt ccagctcggg gctctcgatg tcgaacacgc cgatgcagcg gtccttcacg
     2521 agcagcggga tcaccagctc cgaccgcacg tcgtcgagca ccttgacgta gcggctgtcc
     2581 tgggtgacgt cgggcaccag caccgtccgc ttgtgcagcg ccgcgtgccc caccagcccc
     2641 tcgcccagcc gcaccggcgg caccgtcgtc tgctcgccgt acttgagggc gagcttcatc
     2701 tccagctcgc cggtgtcctc gttgagcagg aggatcccga aggtgcggta gtcgatcacc
     2761 cgcttggcca gctgtgccag gcgggtgagc agctcgtcga gatcgaggat cgacgacacc
     2821 tcgcggccga tctcggcgag cgtctcgagc atctcgttgt actcgcgctc gcgctcgaac
     2881 agccgcgcgt tctcgatgcc gctcgccagc gtcgcggcga actggcggag gatcttctcg
     2941 tccagctcgt tgaactggcc gacgttcggg ctcagcaggt tcagcgcccc gatcaccttc
     3001 cccttgcggc gcagcgggac caccagctcg gaccgggtgc cgggcacgat ctcggtgtac
     3061 cggggatcgg cgctgacgtc gtccacgagg atcgggcgct cgtcgcgcac cgcggcgccg
     3121 atgattccct cgccgatctt gaggcggagc ccttggagcg cctcgggcgg gtagccgacg
     3181 gcgtacgcga tggagaggtc gccgcgcttc tcgtcgatga ggtagaccgc gaacgcctgg
     3241 tagtcggtca gcctgctgat gagctgtggg atgcgctgga gcagctcgtc gaggtcgagg
     3301 acggaggtga cctgccggcc gaggtcgaac agcgtcgtga ggagctgctg gtcgctgggc
     3361 cgctgacact cgctgggatg gccggccggg acttccgacg atggatgcgt cacctgaggc
     3421 ggattatact tgcacggtca tggatctgtc gcttcccctc gaccacccgg cgatcgagcg
     3481 catcctgccg catcgcttcc cgttcctcct cgtggaccgg atcaccgagt tcgagcccga
     3541 caagcgggtc gtcggggtga agaacgtgac gagggacgat cacctgctca ccgaggaacc
     3601 ggggggcgag gccgtcctcc cgccgaccgt gctcaccgag gcggtggccc aggtcggcgc
     3661 gatcctcatc ctggccaagc ccgagaaccg cgagaaactc atctttttcg gcgggatgga
     3721 gagcgtccgt tacaagcggc ccgtcagggc gggcgacgtg gtggtggtcg aggcgagcgt
     3781 ccggcggctt cgcagccgga tgggactgct caagggtgtc gcgaaggtgg acggcaaggt
     3841 gtgcatcgtc ggcaccatga cgttcgcgct gggcgactcg tccaaggaca aggcgtgatc
     3901 cggttccgcc gcccgcgttc caccctcggc cgtgctggca ccgctgttgc agccgagccg
     3961 ttgcgtgcgt tgagttggtc aaccccgtaa cagccgggcc cccgcagggc atgcccggca
     4021 ggagaatgcg gcatgctcga aaccattgcg atcatcctga tcgtcttgtg gctgctggga
     4081 atggtcagcg gctacacgat gggaaacttc atctacatcc tgctggtcat cgccatcgtg
     4141 ctcttcctgg tgcgactggt cagcgggagg cgggtggtct gaccgctccc ttccacagca
     4201 cgcgttcgag ccggccgtgc caggccgtga actcgccgtc ggaggtgtcg tcgccgaggg
     4261 cctgcctcgc catgttgatc ttggcgtcga ggtcgtcggc gaacgagagg atgaacgcct
     4321 cgatcgtcat cggcacgacc ggcgagccga actcgaggca gccgtggtgg gagagaacga
     4381 ggtgttccag ctcggtgagc agcgcgggcg ggaagccgtc gatcgctgac gcggcttccc
     4441 gcaccagcct gacgcccact gcgatgtgcc ccaccagccg tccctcgcgg gagtagctgg
     4501 tggcgggccc gtaatccagc tcgtacagct tgccgatgtc gtgcagcagg gcgccggcca
     4561 ccgcgacgtc gcggttggcc ccatagagcg ccgcgagcga cgagacgacc tcggcgatct
     4621 tcaacacgtg ctcgaggaag ccgccccggt aggcgtggtg cagcgtctgc gcggcgggcc
     4681 acacccggag ccgctcctcg ttctcgcacg cgatcttctc gagcagcgcc ctgatccagc
     4741 cgtcgctgac accatggacg accgcctgca gctcggccca catctcgtcg agcgggcgcg
     4801 gcgccgacag gacgcagtcc tcctcgcgga agccgtcctt ccggtcctga tcggggtgca
     4861 cgcggcgaat gttctcgacg atcagctgga ggcgatcgtt gtagaggttg ccgcgcgccc
     4921 tgaccttgac gaactcgccg gcgtcgaact cccccttcag ccgctccacc tcgtcgaaga
     4981 tcttcgcggc gatgcggccg gtcgcgtcct gcagggccag cgagacgaag tcgccggcac
     5041 gcccctgccg gacgtccttc tggacgcaga ggaagaagcc gacgcccgac gactcggcgg
     5101 taagctgctt gatccgggga agggttgcca tcatgaacga gaggctacca cggtagtcca
     5161 cgaagtcacg aagtcacgag gtcgcgaagt cgtgaggtcg cgaggtcgag tgggtgcgtc
     5221 cagctgagcc gatcccgtgt gatcgccagc tggtaagatc gaggaaagaa atgacagaag
     5281 gaaccgtggt ggaaacggcg gtggatcgcg ccgagaaggt gtcgctgccg gtgacgggca
     5341 tgacctgcgc cgcctgcgcg cgaacgatcc aggtgacgct cgaagacacc cgcggcgtgc
     5401 gccaagccga cgtcaacttc gccaccggcc gggccaccgt cgtcttcgat ccgtcggcgg
     5461 tgaacctcga cggcctggtc ggggcgatcc gcgacgtggg ctacgacgtg ctggacgagc
     5521 ccgacactgg ggacgaggag gcggtggccg acgcacagga acaggcgcac cgcgccgagt
     5581 accgcgccgc ccgccgcaag ttcctcgtcg ccgtcgtttt cgccctgccg atcatggtca
     5641 tcggcatggc tcacctgcag ttcccggggg tgaactgggt gcagctcgcc ctggccctgc
     5701 cggtggtctt ctactcggga gcccagttct accgcggcgc gtgggccgcc gtccgccacc
     5761 gcaacgccga catgaacacg ctgatcgcgg tcggtaccgg cgtggcgttc gcctactcgg
     5821 taatcgtcac ggttgcgccc ggcaccgtca tgcaccccgt gcacgaagga gtgatgagcc
     5881 acgcccccgg cgtctactac gaggtggcga cggcgatcat cgcgctcgtc ctgctgggcc
     5941 ggatgctgga ggcgcgcgca cgcggccgca cctcggaggc aatcaagcga ctgatccacc
     6001 tgcagccgcg cacggcgctc gtcgagcgcg ggggcgtcga ggtggagatt cccgcgcgcg
     6061 aggtgcgccg gggcgacgtc gtgatcgtcc ggcccggcga gcgcctcccg gtggacggcg
     6121 aggtgatcga gggcgcgtcg gcggtggacg agtcgatgct caccggcgag tcgctgccgg
     6181 tggacaaggc gcccggctcg gccgtctatg gaggcagcgt caacagcacg ggcggcttcc
     6241 gcttccgcgc cacgcgggtg ggccgcgaga cgactctcca gcagatcatc aggctggtgc
     6301 aggaggcgca ggcgagccgc gcgccgatcg cccggctggc cgacgtcatc tcgggctact
     6361 tcaccccgat cgtcatcgcc atcgccatcc tcaccttcgt catctggttc gacgtgatgc
     6421 cggcgggcaa ccgcttcacg ccggccctgg tgagcttcgt ggcggtgatg atcatcgcgt
     6481 gcccctgcgc gatgggactg gccacaccca ccgcgatcct ggtgggcacg ggccgcggcg
     6541 ccgagaaggg catcctcatc cgcggtggcg agattctcga gcgcgccggc cacatcacca
     6601 ccgtcgtcct cgacaagacc ggcaccatca ccagcggccg ccccgaggtg accgacctgg
     6661 tgccggcccc cggcatcgtc ccggacgacc tgctcgggct cgccgcctcc gccgaacggg
     6721 cgtcggagca cccgctcgcg caggcgatcg tacgggccgc tggtgaacgc ggcatcccgc
     6781 tgcgcccggt ttccgagttc cgcgcgctgc cggggctggg catcagcgcc gcgaccggcg
     6841 aggggcggct cgtcatcggc aaccagggcc tgatggaccg cgaggggatc gacacctcgc
     6901 cgctcgccgc cgagatggac cgcctcgcgc agcaggcgag aacggtgatg tgggccggga
     6961 tgaacggccg cctggtcggc ctggtgggca tggccgacac cccgagaccc gaatcggccg
     7021 gggccctgcg gcggatgaag gcgatggggc tcgagctggt gatgattacc ggcgacaacg
     7081 ccgcgacggc caacgccatt gccggccagg tagcacccgg gggcgggatc gaccgcgtcg
     7141 tcgccggcgt gctgcccgag cggaaggccc aggaggtcaa ggcgctgcag gcggaaggac
     7201 gcgtggtcgc gatggtcggc gacgggatca acgacgcccc cgcgctcgcg caggccgacg
     7261 tcggcatcgc ggtcgggtcg gggaccgacg tggccatcga ggccgccgac atcacgctga
     7321 tgcgcaacga cctgaacggg gtggccgagg cgattcacct ctcgcgccgc acgctgcggg
     7381 tgatcaagca gaacctgttc tgggcgttca tctacaacgt gctcggcatc ccgatcgccg
     7441 ccggcgcgct ctacccgttc accggctggc tgttgaaccc gatgctggcc gccgccgcga
     7501 tgtcgttctc gagcgtctcg gtgctggcca acagcctgcg gctgagaagg gggtgaggga
     7561 agacggccat gctcgacaac acgcagatcg tcgtctccgt gctgggcctc ggcctgattg
     7621 cgggcgtctt ctggttcttc ttcgggggca agcggaggcg cccgccttcg ccggacgctc
     7681 gccttgctcg cggctacggc ggaaagcccc agagccgtga gccgtgagcc cttgtataat
     7741 cccgccgtac cttcgcgacg cctgcccgcg gccttcgcag gccccgacga gaggtcggcg
     7801 gtggccgcgc gactcttcga tcgcgcgcag gttggcgcgt gcgtcgcagt gcttggccct
     7861 gacggcgccg accgcacgct cgtcgtcaac acccacctga agcacatctt cgggtacgag
     7921 agcagcgtcc ccgagtcgca ggtccttccc ttcgagagcg cccggttcgt cgatccactg
     7981 gcccgctcga acctgctcga gcggctcggg cacgacgggg cggtcgccga cttcctggtg
     8041 cgcgtccgcc gcgtcgactt caccccgatc tgggtcgagg tcaccgccac ggccgagcgc
     8101 caggacggag ggagcctgct ggtggaggcg ctcgtccgcg acgtcagcga ccgcaagaag
     8161 ctcgacgacc agacgcgcga cctgtacgga cagctgctcc aggccgagaa gctggcctcg
     8221 ctgggccaga cgatctcggg catcgcccac gagctgaaca accccctggc gacggtcctc
     8281 acctgggccg agcgcctcgc gccccgccag aaggacgccg acaccaggcg cggactcgag
     8341 gtgatcctct cggaggccga ccgcgccgcc cgcatcgtcc gcaacctgct gaccttcgcg
     8401 cgcaagcgcc acaccacccg gacgatggtc gacctcaacc aggtggtggg cgagacgctg
     8461 gcgctgcgcg cctacgaaca gcgcgtcacc aacatcaacg tcatctcggc gctcgccgcc
     8521 gggctgccgc aggtgttcgc cgacccgcac cagctgcagc aggtgctgct gaacctcgtc
     8581 atcaacgccg agcaggcgat gctggccgcc aacgggcgcg gcacgctggt cgtgcgcacc
     8641 tggcacgatg cctccaccga caccgtcgtt ctcgaggtca acgacgacgg gccggggatt
     8701 gccgaggagc tgcaggcgaa ggtcttcgac ccgttcttca ccaccaagga ggtcggcaag
     8761 gggaccggcc tcggcctcac cgtcgcctac gcgatcgtca aggagcatgg gggccgcatc
     8821 tgggtgaggg gatcgcaggg gcagggggcc tcgttcttcg tcgagctgcc caccggcgag
     8881 gcgcaggtcg tgcgagcccc ccagcctccc cccgagcaga tccgcgccga ggtgccggcc
     8941 gggacgcgcg tgctggtggt cgaggacgag gcggcgctgg cggcggccgt caccgagggg
     9001 ctgagcgacg cgggcttcga ggtggaccac gcctcggacg gggaagaggc gttggcgcac
     9061 gtcgcggcgc gcgcctacga cctgatcgtc tgcgacctca agatgccgcg cgtggacggg
     9121 atggagttct accgggcgat cgcgacgggc aacccggcgc tggcccgccg cgtgatcttc
     9181 tgcaccggcg acgtcgtggg cgccgaagcc gaacgcttcc tcgcggagag cggctgccgc
     9241 tggctcgcca agccgttcag gctcgccgac ttgctgcggg ccgtccgcga gctgctggcg
     9301 tagggcagca ccgtcgccgc caccgccggc agcggctggc ccacgggacg cgccggcagc
     9361 gacgccttcg cgggcaccgg gcgcggctcg aagggaagca ccgggcgcgg cgccatgcgc
     9421 ggacgcggcg tcagcacggt gaggatcggc ggctgcgaca ccggcttctc ctgccgctcg
     9481 agcgcctggc gaaggcgctg gatggcgcgg gcgtgcagct gcgacacgcg cgactcgttc
     9541 acccccagct cggccccgat ctccttcatc gtgacctcgc cgaagtagta gagcctaatc
     9601 agcttgcgct cgcgcgcggg cagcgtggcg atggcggccc gaacccggtc ctgcacctgg
     9661 ccgcgctcga aggccgcgtc gggcgcctcg ggctccgagg gcaccagcac ggccggcagc
     9721 gacgcgtcgt ccttgtgatc gttcgaggcg aggggcgagg tggactcgat ggtgttgatg
     9781 cgcaggatcg tgcggttgag gcgcttctcg tccgagccaa ccttggccgc caggtcggcc
     9841 accgacggct cgtgccccag ctcgcgccgc agcgactcgc gggccgcctc cagctcgcgc
     9901 cgctggcggc ggacgccccg cggccaggcc tcgcgccgaa gcgcgtcgat catcgcgccg
     9961 cgcacccgcc gctccgcgaa ggtctcgaac ttgatgccgc gcgactcgtc gtagcgctgc
    10021 gcggcgtcaa tcaggccgag cacgccgtcc tgcacgagat cgccgatgtc gatcgagtgg
    10081 ggcatcgagg aggcgagcct gcgcgcaagc gcttcgacga agggaagacc ggccaccacg
    10141 cgtcggctct cgttggcggg aagcggtgtg acgtgcatcg cggctcctcg gggcggcgcc
    10201 agccggcgcc actgaacgac ttcggttcga gtctcaggcc gggacgccgt tcggcccccc
    10261 gggtttcgca gacgaaccga gccattcgca acccaagtgc cagaacggaa cagcttggcc
    10321 ccatccggct catccgtgcg tccgaaggcg cgaagccgtt gaaacaagga gggttagaag
    10381 tgactccggc ggccgcgccg ccatcggagc cggtgtgcag atctgttgca gggcgtcaac
    10441 atcttgacga actagcagtt cttgcgcgcc aatattctga cgcaccagca agatctgcaa
    10501 gcgccgcacg aaatcgcaat cgtggtgtga tgtacgaacg gggggccgtg gggtggagcg
    10561 gcggctgaag gaagaaggac ctgcgcggcc tgccgccgcc tgcttcagac ctggaaccgc
    10621 gactggatgt cgctgacccg cgatcggatc gaccgggccg tgcccatcgc gtcgaggacg
    10681 ctggtcacca cctgcgatgc gcgcgagcgg ctgatctcgt cgccggcctt ggcctcgagc
    10741 agctcggcgt gcgacaggat gatgcccagc tcgttgttca gccggtggaa gagcgccccg
    10801 aggtcgccga ggtcgtcggc gggcatgtgc gcgagcgggg tgggcttctg gagagacggg
    10861 ttcatgccgc tatgcgactc cgggggtcct gcttcacagg gcttatcggg aggtccgccg
    10921 tttcccgtta ggccgggatt gcaggacgag tgtaccgaca tttttccgtg ccgggccgcc
    10981 ccggctgtgt caaaatactg gcgcctacct aaatgtctga aaatacaaaa catctcctgt
    11041 acgtcgaaga cgaggtggcg ctgcgccagg tggtggccga gcggctgacc gagcgcgggt
    11101 acgaggtggt cgaggtgtcc aacggcgagg aggccctcga gcggctcgcg gagttcgcct
    11161 tcgatatcgt gatcaccgat cttcgcctgc cgggcatcga cggcgcgcgc gtcatcgagc
    11221 aggcaatcgc cagctacccc gacatcatcg gcatcatcgt gaccggcttc ggcaccgtga
    11281 aggacgcggt ggacgccatc aagcgcgggg ccaccgactt catcaccaag ccgttccagt
    11341 tcgaggagct gatgctggtg ctcgacgccg cgctcgagca gcggcggctg aggtcggaga
    11401 acgcctacct gcggtcgcag ctcgagcagc ggtaccgctt cgaggggatc atcgggcgca
    11461 gccgcccgat gcgaaacctc ttccagctgc tcgagaccgt cgcgcccacc aacagcacca
    11521 tcctggtcac cggcgagacg ggcaccggca aggagctggt cgcgcgcgcc atccaccaca
    11581 acagcccgcg ccgcgccagc cgcttcgtcg cgctcaactg cagcgccatc cccgagacgc
    11641 tgctcgaggc ggagctgttc ggccacgtgc ggggcgcctt caccggcgcc gtcgccaacc
    11701 gtcccggccg cttcgagcag gcccacaagg gcaccctctt cctcgacgag gtggggacga
    11761 tgagcatcgc gctgcagatg aagctgctgc gcgcggtgca ggagcgcgag ttcgagaagg
    11821 tgggcgattc gcgccccacc aaggtggacg tgcgggtgat cgccgccacc aacagcgacc
    11881 tcgcgcagat ggtgcgcgac gggacgttcc gcgaagacct ctattaccgg ctgaacgtga
    11941 tcccgatcca cctgccgccg ctgcgcgagc ggcgcgagga catacccctc ctggttcagc
    12001 acttcctcga tcggttctgc cgcgagctgg tgccgtcgcg cccggcgctc accgtgtcgc
    12061 agcacgcgat gcggcggttg atggggttct cgtggccggg caacgtgcgg cagctcgaga
    12121 acgccctcga gcgcgcggtc gcgctgagcg gcgggcgcga tcagatcgag gtggccgacc
    12181 tcccgcccga cgtgcaggag gtgggcggcg ggtcgatgcc ccaggagttc gcgcttcccg
    12241 aggaagggat cgacttcgag gagtacgtct cgaaggtgga gctggatctc atcaagcagt
    12301 cgctcgagcg gaccggcggg aacaaggggc gcgccgcgca gctgctgaac ctcaagcgca
    12361 cgacgctcgt cgagaagctc aagaggctca acgagtcgta gcggccgaca aggatcctat
    12421 gccaccccgt ttcgccttct ggacgatcgt catcgacggc tcgcccacgg cctttcgcgc
    12481 ggcgaagcgc gacgacctgc tccccacgct gaagcagctg caggggagga acccgcacgc
    12541 cgagctgaag tggttcgctc gcggcaaggt ctgggagtcg cccgagcagg cccgcgcctc
    12601 gcgcgagcgt ggcggcccgc cgcgacggga ctctttcgcg cacggcggcg caggcggcgc
    12661 cggggacgag cgccgcggca agcagtggcg gccgggcggc gagcaccgcg acccgcgcga
    12721 gaagttcaag aaggaaacct accaggcgag gaagcggcgc gagaagaagg cagccgcgct
    12781 ggcccggcag ggcggggccg ggttcccgcc acccggtggg aagactcacg gcggtcgcga
    12841 tcgccgcgaa ccgcaggatc gcccctggaa gccgaatcgt ccgcgcgagg gtcggccgcc
    12901 gcaggaacag ccctggaggc cgcagcgccc tcgtgaaggc agc
//
LOCUS       DAOPQX010000067        12880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5654_length_12880_cov_4.398519, whole genome shotgun sequence.
ACCESSION   DAOPQX010000067 DAOPQX010000000
VERSION     DAOPQX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5654_length_12880_cov_4.398519"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /gene="fahA"
                     /locus_tag="PKK95_05505"
     CDS             complement(<1..1114)
                     /gene="fahA"
                     /locus_tag="PKK95_05505"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HOC17701.1"
                     /translation="MTDADLLDDTHDPARTSWVESANDPSTDFPLQNLPVGWGQPRDG
                     GPPRVLAAIGDRVLDLAGARGEGWLPGVPAAVLEALDGPRLNALMACTPGGRRALRQA
                     LAGLLGTRPPAPARRIEAHLLPAEAVEMLVPASIGDYTDFYASVFHAERVGRLFRPDN
                     PLLPNYKHVPIGYHGRASSIVASPAGVVRPLGQTRPEGASAPRFGRSARLDFELEVGL
                     LIGTGNALGERVPVDRAEEHAFGLCLVNDWSARDVQQWEYQPLGPFLAKSFATTVSPW
                     VVTLEALAPFRAPAFARPPGDPVPLAYLQPGPAPGAFDVTLEARLSSQAMREQGLEPA
                     RVTRSRLADLYWTFAQMIAHHTSNGCNLRPGDLLASG"
     gene            complement(1111..2049)
                     /locus_tag="PKK95_05510"
     CDS             complement(1111..2049)
                     /locus_tag="PKK95_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17702.1"
                     /translation="MQGAAASLAALLAGAIDYAGLFPPASLAIDAAAGVYLRHLAGPH
                     RGLLGRFVVPAAQLDALAGAARALNPRPASGDGPGRWPLTALASLPAAADVERILRFN
                     ERHAEGHEWRARVEAIEVRAATPDETRAAARDAGGLELFVEVPAHPVPLALLEAVVEA
                     GGRAKVRTGGAKPDAFPPPGHLAAWLAYCAGLRLPFKATAGLHHPIRAVRPLAGSADS
                     PRAAMHGFVNVLMAAALLRADRIDAQKAAALLDERDPAAFAFDDQQAAWRGESLATTE
                     LADARAFALGFGSCSVDEPIEDLAALGLLTSAPPAS"
     gene            complement(2050..3207)
                     /locus_tag="PKK95_05515"
     CDS             complement(2050..3207)
                     /locus_tag="PKK95_05515"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HOC17703.1"
                     /translation="MPIYHRLGQLPRKRHTAFRDQDGRLRTEELIGNEGFTGPASLVY
                     HIHPPTRLKEVRPVRSLDWQAEPDRMLRHRHFKTAGLTTGPSPVFDRVPLLFNADVAI
                     ALVRASADDETFYRNAQGDEIVYVTAGAGVLDTPLGTLKFGEGDYLVIPRGILHRYRF
                     EQRPSTLLVIESAGYVRTPKRYRNAFGQLLEHSPFCERDIRRPEWADPRDERGDFRLV
                     VKKNNALVEMTLAHHPCDVVGWDGYYYPWAFNIHDFEPRVGRFHLPPPVHQTFEGDGF
                     VVCSFCPRPYDFGEDAVPAPYNHSNVMSDEVLYYVSSDFMSRKGIEFGSITLHPDGLP
                     HGPHPGRTEASIGQKGTAEVAVMLDTFRPLAVSTQARTIEDRDYYRSWYEE"
     gene            complement(3243..4331)
                     /gene="hppD"
                     /locus_tag="PKK95_05520"
     CDS             complement(3243..4331)
                     /gene="hppD"
                     /locus_tag="PKK95_05520"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HOC17704.1"
                     /translation="MQQNPLRLRSIHHVEFWVGNARQAAYFYRLGFGFSQAAYAGLET
                     GARDHTSYLMTQGKIRLVLTTPLGPDHPAATHILAHGDGVRDIAFHVDDADRAFEEAV
                     RRGAEPAFEPHDLRDGNGCVRRAAIKTYGDTIHSFISDAGYRGPFLPGYSERRVEGRD
                     IGLLRVDHMVGNVELGKMNEWADWYHRVLGFERFVSFDDKDISTDYSALMSVVMADDA
                     QAIKFPLNEPAPGKKKSQIDEYLEYYRGPGVQHVALLTTDIVATVDALRQNGVEFLRV
                     PDSYYDLLPGRVGAIEESVDELRRLGVLVDRDEEGYLLQLFTRPVQDRPTLFFEIIER
                     KGSRGFGKGNFRALFEAIEREQALRGNL"
     gene            4432..4764
                     /locus_tag="PKK95_05525"
     CDS             4432..4764
                     /locus_tag="PKK95_05525"
                     /inference="COORDINATES: protein motif:HMM:NF021188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOC17705.1"
                     /translation="MYIEEPHELGQQEAIERIDSFLEALMERQPPGGVTIKNPTKRWD
                     GNTMHFSFTAAKGFFGTTIRGLLRVEDDRVVMDSELPGLVRSVVGEDRIREVISRELG
                     RLLGEPRG"
     gene            4761..6890
                     /locus_tag="PKK95_05530"
     CDS             4761..6890
                     /locus_tag="PKK95_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HOC17706.1"
                     /translation="MSAKRVPGARAGRRAPARPQCPIDRWREVEGSPSPLGATFLADA
                     DAWNFAVYSKHATAVTLLLYGPHDHARPLLSVRFDYLRNKSGRVWHGRLPAAQVRGAT
                     HYAYVVEGPFDPAGGQRFDRDKILLDPYARAVFFPPTFSRDAAMRPGPNAGRAPLGVL
                     GPLAPPNGDGDGGCRPRHAWDTVIYEMHVRGFTRRANSGVVPGRRGTFAGVIDKIPYL
                     LDLGVTAVELLPVFQRDPQEDNYWGYMPLNFFAPHYQYAAAGTPEGAVEEMAAMVRAL
                     HEAGIEVILDVVYNHTVEGDERGPTYSYRGLDNSTYYLLRQDDRSQYRNDTGTGNVLH
                     TANLAVRKMVVESMRFWARQMHVDGFRFDLASIFTRRTDGSIDLQDPPMISAIDTDPD
                     FANIRLIAEAWDLHSYQLGRRFPGVEWLQWNDTFRDDVRAFVRGDEGMVGRLATRLYG
                     SSDQFPDERLDAYHAFQSVNLVTAHDGFCLYDLVSYNRKHNLANGEENRDGTDYNLSW
                     NCGWEGDIGAPPAVLALRKQQVRNFCCLLFLANGTPMFCAGDEFLQTQGGNNNPYNQD
                     NETTWLDWDLLKKNADIFRFFRMMIAFRKAHPSIARSRFWRDDVRWYGPEGEPDWGPR
                     SAKLAYCLHGASQGDEDLYVMINGGTEDAWFAVQEGAPRQWRRVFDTSRPSPDDAVDP
                     AEAETLRSLQYLVRARSVVVLLGEERK"
     gene            complement(6898..9000)
                     /locus_tag="PKK95_05535"
     CDS             complement(6898..9000)
                     /locus_tag="PKK95_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC17707.1"
                     /translation="MPENGSFADTDLVDSLPTGVFVCEATEGRLIRYNRCAAQLWGRE
                     PPLGGDARFDGAFRLYTPEGHPLPHERNPVAEVLRTGRPVGDTDVVVERPDGSRITVV
                     LNASPLRQSTGRLSGAVGVFHEVSERRRGEERERLLLQASTVLGSTLDHQAILHQLAR
                     LALPRYADWCLLEVVEDDGQVGVVAGAHVHPEREELVIELAGQAGALGEAAAAVLRTG
                     EPFLLAMVPRGFAGSVARTDRQFHLLKALSPESLIVAPLKARGKVMGLIAFVFSGSGR
                     RYQPADVPLAEGVARRAALAIDNARLYRDSQQANRLKDEFLATLSHELRTPLNAVLGW
                     TQLLLTRQLRGEEVDRALQTIRRNAEAQAHLIGDILDVSRIITGKLRLNIAPTDLHTV
                     IHAAVQAVRPAAEAKGLVLTVDSEASPRAMLADPDRLQQVVWNLLSNAVKFTPSGGRV
                     TLQVSAAAGTTQVRVSDTGAGIPPRFLNSLFERFRQADGSTTRPYGGLGLGLAIVRHL
                     VELHGGSVRGESEGEGRGATFTVTLPVGRTEPRAVHASLPLAAPRAAPRDEAVNLDGV
                     RALVVDDDEDSRHLTRAVLEGLGARVGEASSVPAAIDAMRQEWPEVLLADIGMPGEDG
                     YSLIGWVRRIERQSASRLPALALTAYARSADRERALEAGFDEYLSKPASPTDVAQAVG
                     RLTGRTTGYERENVKGKM"
     gene            complement(9193..10683)
                     /locus_tag="PKK95_05540"
     CDS             complement(9193..10683)
                     /locus_tag="PKK95_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOC17708.1"
                     /translation="MMGFSLVDYLVVVLYLVGVTVFGAQFRRTQRSVKDYFLGTRNTH
                     WLIISLSIVATETSSLTLIGVPAIAYGTYARPELGGSLTYLQVVLGYIVARVIISFLL
                     IPAYFQGEMVTAYELLRRRFGSRAKHFAATLFVVTRAFADGVRVFAASLVAAAVIGSS
                     LPNFPDLWFWSVVMVGVLTLIYTFEGGVAAVIWTDLIQLVVYVSGSLLAAYMLLDLVP
                     GGWATVASHAADANKFQLFSFSWDLSLPFTFWAGLIGGTFLTMASHGTDQLMVQRLLT
                     CRNQRDGQKALIASGLIVLVQFLLFLVVGVMLYAYYQHVPAPRLTTNDEIFPAFIVAH
                     LPRGISGLMIAAIFAAAMSTMSGSLNSLASTTIFDFYKPLLRRKVEDAELLRLSRWVT
                     AGWGVILIAIALAARDWGSVFVAGLTIASLVYGPMLGAFLLGVLTTRATEAGVMIGIA
                     VSLASMVAVWLWTPLAWTWYVLAGTAICMTVGYAASLLGGPARSQS"
     gene            10816..12075
                     /locus_tag="PKK95_05545"
     CDS             10816..12075
                     /locus_tag="PKK95_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOC17709.1"
                     /translation="MTHHSPQIPAELVECEARRSETLHEVRCEDCLGCDFECKVMQAD
                     LERCVAEVLRAHKLPARDAQITARVLVAADLRGIASHGVARLGRYVKGLQEGYIVPGV
                     AFDVREPAPAVAAIDAKNGVGQVVSELAMDMAVARARAHGIGVVTVANSNHYGIAGYY
                     VQKAIDAGMIGLSLTNAAPLVVPTNGADAVLGTNPIAFGAPGKDGFDFLLDMATSVVP
                     RGKLEVYDRNRRQMPVGWAVDEKGYDTRNPGHVLANLLARSGGGILPLGGRGEDFSGH
                     KGYGLALMVDVLAGVLSGSAFGADVDNVRGAPGSGPGSAPRVGHFFLALDIARFMPLE
                     EFRDRLAALFAMLKDSRKALDKSVIYIHGEKEQARTRLHEQSGIPLSENVMASLRKIA
                     ADCGAAPPITVADRLRRKVTAPEEGLS"
     gene            12072..>12880
                     /gene="lhgO"
                     /locus_tag="PKK95_05550"
     CDS             12072..>12880
                     /gene="lhgO"
                     /locus_tag="PKK95_05550"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOC17710.1"
                     /translation="MSAAWDVAIIGGGIVGTATAMALARDAGASVVVLEAEDRLAAHQ
                     TGHNSGVIHSGLYYKPGSLKARNCVEGREAMYRFCEAHQIPHERCGKLVVATRTEHLA
                     PLDELERRGRANGLEGLRRLAGDELRDYEPEVAGIAGLHVPQTGIVDYTAVTEAFARL
                     VREAGSEIRTSSRVLRVERRPGAVVLETPGGAVEARALINCAGLQSDRVARMCGVDPG
                     VQIVPFRGEYYELVPGREHLVRNLIYPVPDPRFPFLGVHFTRMIHGGVEAGP"
BASE COUNT         1974 a         4453 c         4675 g         1778 t
ORIGIN      
        1 ttccgctcgc cagcaggtcg ccgggccgca ggttgcaccc gttgctcgtg tggtgcgcga
       61 tcatctgtgc gaaggtccag tagaggtccg ccaggcggct tcgcgtcacg cgcgccggct
      121 cgagcccctg ctcgcgcatc gcctgcgacg agaggcgggc ctcgagcgtg acgtcgaagg
      181 cgccgggcgc gggacccggc tgcaggtatg ccagcggaac gggatcgccg ggcggccgcg
      241 cgaaggcggg cgcgcggaac ggcgccagcg cctccagcgt gaccacccag ggcgagacgg
      301 tggtcgcgaa gctcttcgcc aggaacgggc cgagcggctg gtactcccac tgctggacgt
      361 cgcgggccga ccagtcgttc accaggcaga ggccgaacgc gtgctcctcg gcccggtcga
      421 caggcacccg ttcaccgagc gcgttcccgg tgccgatcag cagtcccacc tccagttcga
      481 agtccagccg cgccgatcgt ccgaagcgcg gggcggaggc gccctcgggg cgggtctgtc
      541 cgagcggccg cacgaccccg gcggggctgg ccacgatcga cgatgcgcgc ccgtggtagc
      601 cgatcggcac gtgcttgtag ttgggcagca gcgggttgtc ggggcggaac aatcgtccca
      661 cccgctcggc gtggaagacc gacgcgtaga aatcggtgta gtcgccgatc gaggctggca
      721 ccagcatctc gacggcctcg gccggcagca ggtgcgcttc gatccgccgg gccggcgccg
      781 gcggcctggt gcccagcagg ccggcgaggg cctgccgcag ggcgcggcgc ccgcctggcg
      841 tgcacgccat caaggcgttc aggcgcgggc cgtcgagcgc ctcgagcacg gcggccggca
      901 cgccgggaag ccacccctcg ccgcgggcgc ccgccaggtc gagcacgcgg tcgccgatgg
      961 ctgccagcac ccgcggcggc ccgccgtccc gcggctggcc ccagccgacc ggaaggttct
     1021 gcagcgggaa gtcggtggac ggatcgttcg ccgattccac ccagctggtg cgggcgggat
     1081 cgtgggtgtc gtcgagcagg tcggcgtcgg tcatgatgcg ggcggcgccg aggtgagcag
     1141 gcccagcgcc gcgaggtcct cgattggttc gtcgaccgaa caggagccga aacccagggc
     1201 gaacgcccgg gcgtcggcca gctccgtcgt tgccagcgat tcgccgcgcc aggccgcctg
     1261 ttggtcgtcg aaggcgaagg cggccgggtc gcgctcgtcg agcaaggccg ccgccttctg
     1321 cgcgtcgatc cggtcggcgc gaagcagtgc ggcggccatc aacacgttga cgaagccgtg
     1381 catcgcggcg cgcggagagt cggcgctgcc cgcgagcggc cggacggcgc ggatcgggtg
     1441 gtgaaggccg gcggtggcct tgaagggcag cctgagcccc gcgcagtacg ccagccaggc
     1501 ggccaggtgg ccgggcggcg gaaacgcgtc gggcttcgcg ccgccggtgc gaaccttcgc
     1561 tcggcctccc gcctcgacca ccgcctcgag cagcgccagc ggcaccgggt gggcgggcac
     1621 ctccacgaac agctccagcc cgcccgcgtc ccgtgccgcg gcgcgcgtct cgtccggcgt
     1681 cgccgccctc acctcgatcg cctccacgcg cgcccgccat tcgtgcccct cggcgtggcg
     1741 ctcgttgaag cgcaggatgc gttcgacatc cgcggcggca ggaagcgacg cgagggcggt
     1801 cagcggccag cgtcccgggc cgtctccgga ggcgggccgc gggttcagcg ccctggcagc
     1861 cccggcgagc gcgtcgagct gcgccgccgg cacgacgaag cggcccagca ggccgcggtg
     1921 cggtccggcc aggtggcgga ggtagacgcc tgccgcggcg tcgatcgcga gcgaggccgg
     1981 ggggaagagc cccgcgtagt cgatcgcccc cgccagcagt gccgcgagcg acgcggcagc
     2041 gccctgcatc tactcctcgt accaggagcg gtagtagtcg cggtcctcga tggtccgcgc
     2101 ctgcgtgctg acggcgagcg gccggaaggt gtcgagcatc accgccacct ccgcggtgcc
     2161 cttctggccg atcgacgcct cggtgcggcc cgggtgcggc ccgtgcggga ggccgtcggg
     2221 gtgcagcgtg atcgatccga actcgatccc cttgcggctc atgaagtcgg agctgacgta
     2281 gtagaggacc tcgtcggaca tcacgttcga gtggttgtag ggggcgggca ccgcgtcctc
     2341 gccgaagtcg tacgggcgcg ggcagaacga gcagacgacg aaaccgtccc cctcgaaggt
     2401 ctggtgcacc ggcggcggga ggtggaaccg tcccacgcgc ggctcgaagt cgtggatgtt
     2461 gaaggcccag gggtaatagt acccgtccca cccgacgacg tcgcacgggt ggtgcgcaag
     2521 ggtcatctcg accagcgcgt tgttcttctt caccacgagg cggaagtccc cgcgctcgtc
     2581 tcgcgggtcg gcccactccg gccgccggat gtcgcgctca cagaacgggc tgtgctcgag
     2641 gagctgcccg aaggcgttgc ggtatcgctt cggcgtgcgc acgtacccgg cgctctcgat
     2701 gacgagcagc gtggacggcc gctgctcgaa gcggtagcgg tggaggatgc cgcgcgggat
     2761 caccaggtag tcgccttcgc cgaacttcag cgtcccgagc ggcgtgtcga gcacgccggc
     2821 gcccgcggtg acgtacacga tctcgtcgcc ctgcgcgttc cggtagaacg tctcgtcgtc
     2881 ggccgacgcg cggacgagcg cgatggcaac gtcggcgttg aagagcagcg ggacgcggtc
     2941 gaagaccggg ctggggccgg tggtcaggcc cgccgtcttg aagtgacggt gccgcagcat
     3001 ccggtcgggc tcggcctgcc agtcgagcga tcggacgggc cgaacctcct tcagccgggt
     3061 gggcgggtgg atgtggtaca cgagcgaggc agggccggta aatccctcgt tgccgatcag
     3121 ctcctcggtc ctgaggcggc cgtcctgatc gcgaaacgcc gtgtgccgct tgcggggcag
     3181 ttggccgaga cgatggtaga tgggcatttc gcttcccttc aggccgatcg tcgaacggcc
     3241 cgtcagaggt tgccgcgcag cgcctgctcg cgttcgatgg cctcgaacag cgcccggaag
     3301 ttgcccttgc cgaacccccg gctccccttg cgctcgatga tctcgaagaa gagggtcggg
     3361 cggtcctgca cggggcgggt gaagagctgc aggagataac cctcctcgtc gcggtcgacc
     3421 agcacgccca gccggcgcag ctcgtccacc gattcctcga ttgcgccgac ccggcccggg
     3481 agcaggtcgt agtagctgtc gggcacgcgg aggaactcga cgccgttctg ccggagcgcg
     3541 tccacggtgg cgacgatgtc ggtcgtcagc agggccacgt gctgcacgcc gggcccacgg
     3601 tagtactcga ggtactcgtc gatctggctc ttcttcttgc cgggtgccgg ctcgttcagc
     3661 gggaacttga tcgcctgggc gtcgtcggcc atgacgacgc tcatcagggc gctgtagtcg
     3721 gtggagatgt ccttgtcgtc gaagctgacg aaccgctcga acccgaggac gcggtggtac
     3781 cagtcggccc actcgttcat cttcccgagc tcgacgttgc cgaccatgtg gtcgacgcgc
     3841 agcagcccga tgtcgcgccc ttcgacccgc cgctcggagt acccggggag gaacggcccg
     3901 cggtagcccg cgtccgagat gaacgagtgg atcgtgtctc cgtaggtctt gatggcggcg
     3961 cggcgtacgc acccgttccc gtcgcgcagg tcgtgcggct cgaaggccgg ctcggcgccc
     4021 cggcgcacgg cctcctcgaa cgcccggtcg gcatcgtcga cgtggaaggc gatgtcgcgc
     4081 accccgtcgc cgtgggcgag gatgtgggtg gcagccggat ggtcggggcc gagcggagtg
     4141 gtcagcacca ggcggatctt gccctgcgtc atcaggtagg atgtgtgatc gcgggcgccg
     4201 gtctcgaggc cggcataggc ggcttgcgag aagccgaacc cgagccgata gaagtacgcg
     4261 gcctggcggg cgttgccgac ccagaactcc acgtggtgga tgctcctgag gcggagcggg
     4321 ttctgctgca tgggtgtgcc cctccgacta aggtggcaca gacgagccgc ggccggcaag
     4381 agacgaacgg gaccgggccc ccgacagctg agcggggacg caggagaggc catgtacatc
     4441 gaggaaccgc acgagctcgg ccagcaggaa gccatcgaac gcatcgacag cttcctcgag
     4501 gctctgatgg agcggcaacc cccgggcggc gtcacgatca agaacccgac gaagcgctgg
     4561 gacggcaaca cgatgcactt ctcgttcacc gccgcgaagg gcttcttcgg gacgaccatt
     4621 cgcggcctgc tccgcgtgga ggacgaccgg gtggtgatgg actccgagct gccggggctg
     4681 gtgcgcagcg tggttggcga ggaccggatt cgcgaggtga tctcgcgcga gctgggacgc
     4741 ctgctgggcg agcctcgcgg atgagcgcga agcgggtgcc gggggcgcgc gccgggcgcc
     4801 gcgctccggc caggccccag tgccccatcg accggtggcg cgaggtggag gggtcgccct
     4861 cgccgcttgg agccaccttc ctcgcggacg ccgacgcctg gaacttcgcg gtctactcca
     4921 agcacgcgac cgcggtcacg ctgctgctct acggccccca cgaccacgcg cgccccctgt
     4981 tgtcggtgag gttcgactac ctgcgcaaca agtcggggcg cgtctggcac ggccgcctgc
     5041 cggcggcgca ggtgcgcggc gctacccact acgcgtacgt ggtcgagggc ccgttcgacc
     5101 cggccggggg ccagcgattc gaccgcgaca agatcctcct cgacccgtac gcgcgggcgg
     5161 tgttctttcc gccgaccttc agccgcgatg cggccatgcg cccgggcccg aacgcggggc
     5221 gcgcgccgct cggggtgctc gggccgctcg cgccgcccaa cggggacggc gatggcggct
     5281 gccgcccgcg gcacgcctgg gacacggtca tctacgagat gcacgtgcgc ggcttcaccc
     5341 gccgggccaa ctccggggtc gtccccggcc gccgcggcac cttcgccggc gtgatcgaca
     5401 agatcccgta cctgctggat ctcggcgtca ccgcggtgga gctgctgccg gtcttccagc
     5461 gcgatccgca ggaggacaac tactggggct acatgccgct caacttcttc gcgccgcact
     5521 accagtacgc ggcggccggc acgcccgaag gggccgtgga ggagatggcg gcaatggtgc
     5581 gcgcgctgca cgaggccgga atcgaggtca tcctcgacgt cgtctacaac cacaccgtcg
     5641 agggggacga gcgagggccg acctactcct accggggcct cgacaactcg acctactacc
     5701 tgctgcggca ggacgaccgc tcgcagtacc ggaacgacac gggcaccggc aacgtgctcc
     5761 acaccgcgaa cctcgccgtc aggaagatgg tggtcgagag catgcggttc tgggcgcgcc
     5821 agatgcacgt ggacggcttc cgcttcgacc tcgcgtcgat cttcacgcgc cgcaccgacg
     5881 ggtcgatcga cctgcaggac ccgccgatga tctcggcgat cgacaccgac cccgacttcg
     5941 cgaacatccg gctgattgcc gaggcatggg acctgcacag ctaccagctg gggcgacgct
     6001 tccccggtgt cgagtggctg cagtggaacg acaccttccg cgacgacgtg cgggccttcg
     6061 tgcgcggcga cgaggggatg gtcggccggc tcgccacccg cctctacggc tcgagcgacc
     6121 agttccccga cgagcggctc gacgcctacc acgcgttcca gagcgtcaac ctggtcacgg
     6181 cccacgatgg cttctgcctg tacgacctgg tgtcgtacaa ccgcaagcac aacctcgcca
     6241 acggcgagga gaaccgcgac ggcaccgact acaacctgag ctggaactgc ggctgggagg
     6301 gggacatcgg ggcgccgccc gcggtgctcg cgctgcggaa gcagcaggtc cgcaacttct
     6361 gctgcctgct gttcctcgcc aacggcacgc cgatgttctg cgccggcgac gagttcctcc
     6421 agacacaggg cggcaacaac aacccgtaca accaggacaa cgagacgacc tggctcgact
     6481 gggacctgct gaagaagaac gccgacatct tccgcttctt ccggatgatg atcgccttcc
     6541 gcaaggctca cccctcgatc gcgcgcagcc gcttctggcg cgacgacgtg cggtggtacg
     6601 gcccggaagg cgagcccgac tggggaccgc ggtccgcgaa gctcgcctac tgcctgcacg
     6661 gagcgtcgca gggcgacgag gatctgtacg tcatgatcaa cggcggcacc gaggacgcct
     6721 ggttcgccgt ccaggaaggg gcgccgcgcc agtggcggcg ggtgttcgac acctcccgtc
     6781 ccagccccga cgacgccgtc gacccggcgg aggcggaaac gctccggtcg ctgcagtatc
     6841 tcgttcgggc ccgctcggtc gtcgtgctgc tgggggagga acggaagtga gagggaacta
     6901 catcttccct ttcacgttct ccctttcata ccctgtcgtc cgccccgtca ggcgtcccac
     6961 tgcttgggcc acgtcggtgg gggaggccgg tttggagagg tactcgtcga aacccgcctc
     7021 gagcgcccgc tcgcggtcgg ccgagcgcgc gtacgcggtg agtgccagcg cgggcaggcg
     7081 gctggcgctc tgccgctcga tccgccgcac ccacccgatg agcgagtagc cgtcctcgcc
     7141 cggcatgccg atgtcggcca gcagcacctc gggccactcc tggcgcatcg cgtcgatggc
     7201 ggccggcacc gaggacgctt cgcccacccg cgcgccgaga ccttcgagca cggcgcgtgt
     7261 caggtggcgg ctgtcctcgt cgtcatcgac caccagcgcc ctgacgccgt cgagattcac
     7321 cgcttcgtcg cggggcgcgg cgcgcggggc ggcgagcggg agggacgcgt ggacggcccg
     7381 tggctcggtc cggccgaccg gcagcgtcac cgtgaaggtc gccccgcgtc cctctccctc
     7441 gctctcgccg cgcaccgacc cgccgtgcag ctcgaccagg tggcgcacga tcgcgagccc
     7501 aagacccagc cctccgtacg ggcgcgtggt ggagccgtcg gcctggcgga agcgctcgaa
     7561 cagcgagttg aggaagcgag gcgggatgcc ggcccccgtg tccgacaccc gcacctgcgt
     7621 cgtgccggcg gcggcgctga cctgcagcgt gacccgcccg cccgacggcg tgaacttcac
     7681 cgcgttcgag agcaggttcc agacgacctg ctgcaggcgg tcgggatcgg cgagcatcgc
     7741 ccgcgggctg gcctcgctgt ccaccgtgag caccaggccc ttggcctcgg cggccgggcg
     7801 cacggcctgg acggccgcat ggatcaccgt gtgcagatcg gtgggcgcga tgttcaggcg
     7861 cagcttcccg gtgatgatcc gggaaacgtc gaggatgtcg ccgatcaggt gcgcctgcgc
     7921 ctcggcgttg cggcggatgg tctgcagggc ccggtccacc tcctcgccgc gcagctgacg
     7981 cgtcagcagc aactgcgtcc agccgagcac ggcgttcagc ggcgtcctca gctcgtggga
     8041 gagcgtcgcg aggaactcgt ccttcagccg gttcgcttgc tgcgagtcgc ggtagagtcg
     8101 cgcgttgtcg atggcgagcg ccgcgcgccg cgcgaccccc tcggccagcg gcacgtcggc
     8161 cggctggtac cgccggcccg aacccgagaa gacgaaggcg atgaggccca tcaccttccc
     8221 gcgcgccttg agcggggcga cgatcagcga ctcgggcgag agcgccttca gcagatggaa
     8281 ctggcggtcg gtccgcgcca ccgagccggc aaacccgcgc ggcaccatcg ccagcaggaa
     8341 cggctccccg gtccggagca ccgccgcggc cgcctcgccg agggcgcccg cctgtccggc
     8401 cagctcgatg acgagctcct cgcgctcggg atgcacgtgc gcgcccgcca ccaccccgac
     8461 ctggccgtca tcttcgacga cctcgagcag gcaccagtcc gcgtagcggg gaagggccag
     8521 ccgggcgagc tgatgcagga tggcctggtg atcgagggtc gaccccagca cggtgctcgc
     8581 ctgcagcagc agccgctcac gctcctcgcc ccgccggcgc tcgctcacct cgtggaacac
     8641 gccgacggct ccgctgagac ggccggtgga ctggcggagc ggcgacgcgt tcaggacgac
     8701 ggtgatgcgc gacccgtcgg gacgctccac cacgacgtcg gtgtcgccca ccggacggcc
     8761 ggtccggagg acttcggcaa ccgggttgcg ctcgtgggga agcggatgtc cctcgggggt
     8821 gtacaggcgg aaggcgccgt cgaagcgcgc atcgccgcca agcggcggct cgcgccccca
     8881 cagctgggcc gcgcaccggt tgtagcggat caggcgaccc tcggtcgcct cgcaaacgaa
     8941 aacacctgtg ggtagcgaat cgacaagatc agtgtcggcg aaggacccgt tttctggcac
     9001 gcagaaaccc tggccgggct tggtgcaagg tccgggccag tgccaggccg gcactgaaca
     9061 gccgggccgg ccctcgccag gggggcgttt tcggggcgtc ccagcccccg gcaggtgccg
     9121 gtgagccgcg tcgaggtcga tgccagccgc gccaagcccg ggcgcccgaa aacccgcccc
     9181 gggcgggcag agctacgact gactcctggc agggcctccg agcaggcttg cggcgtagcc
     9241 caccgtcatg cagatggcgg tccctgccag cacgtaccag gtccaggcca gcggcgtcca
     9301 gagccagacc gccaccatgc tcgccagcga cacggcaatg ccgatcatca cgcccgcctc
     9361 ggtggcgcgc gtggtgagca ctccgaggag gaaggcgcca agcatcgggc cgtacaccag
     9421 cgaggcgatg gtgagcccgg ccacgaacac cgagccccag tcgcgtgccg ccagcgcgat
     9481 cgcgatgagg atcacgcccc accccgcggt cacccagcgc gacaggcgga gcagctcggc
     9541 gtcctcgacc ttccgccgga gcagcggctt gtagaaatcg aagatggtgg tcgaggcgag
     9601 cgagttcaac gatccgctca tcgtggacat cgccgccgcg aagatcgcgg cgatcatcag
     9661 gccggagatc cccctcggca ggtgcgccac gatgaaggcg gggaagatct cgtcgttggt
     9721 ggtcagccgc ggcgcgggca cgtgctggta gtacgcgtag agcatcacgc cgaccacgag
     9781 gaagagcagg aactgcacga ggacgatcaa gccgctggcg atcagcgcct tctggccgtc
     9841 gcgctggttc cggcaggtga gcaggcgctg caccatcagc tggtcggtgc cgtgcgaggc
     9901 catcgtgagg aacgtcccgc cgatcagtcc cgcccagaag gtgaagggga gagagaggtc
     9961 ccacgagaag gagaacagct ggaacttgtt cgcgtcggcg gcgtgcgagg ccacggtcgc
    10021 ccagccgccg gggaccagat ccaggagcat gtaagccgcc agcagcgagc ccgacacgta
    10081 gacgacgagc tggatcaggt cggtccagat cacggccgcc acgcccccct cgaacgtgta
    10141 gatcaacgtc agcacgccga ccatcaccac cgaccagaac cacaggtcgg ggaagttcgg
    10201 gaggctgctg ccgatcaccg cggcggccac cagcgaggcc gcgaacacgc ggacgccgtc
    10261 ggcgaaggcg cgcgtcacga cgaagagggt ggcggcgaag tgcttcgcgc ggctgccgaa
    10321 gcggcggcgc agcagctcgt aggccgtcac catctcgccc tggaagtagg ccggaatcag
    10381 caggaagctg atgatgacgc gggccacgat gtagccgagc acgacctgga ggtaggtgag
    10441 cgagcccccc agctcgggcc gcgcgtaggt gccgtaggcg atggccggca cgccgatcag
    10501 ggtcagcgag ctggtctcgg tcgccacgat cgagaggctg atgatcagcc agtgggtgtt
    10561 gcgcgtcccg aggaagtagt ccttgacgga gcgctgcgtc cgccggaact gggcgccgaa
    10621 cacggtgacg ccgaccaggt agaggacgac gacgagatag tcaacgaggg agaagcccat
    10681 catgcatccc cgaaatcgcg atcggaaccc catccccatg gggccggcgg ccgaccgggc
    10741 agtatagctc ggcacgagcg tctttcgcct acctcagcga atgtgacaca gtagtagacg
    10801 ggaggagtca ggggcatgac tcaccattcg ccacagatcc cggcggagtt ggtggagtgt
    10861 gaggcgcgtc ggtcggagac gctccacgag gtgcgctgcg aagactgcct gggctgcgac
    10921 ttcgagtgca aggtgatgca ggccgatctc gagcggtgcg tcgccgaggt gctccgggcg
    10981 cacaagctgc cggcgcgcga cgcccagatc acggcgcggg tgctggtggc cgccgacctg
    11041 cgcggcatcg ccagccacgg cgtcgcccgc ctcgggcggt acgtgaaggg gctgcaggag
    11101 ggctacatcg tgcccggcgt cgccttcgac gtgcgcgagc cggcgccggc cgtcgccgcc
    11161 atcgacgcga agaacggcgt cggccaggtg gtgtcggaac tggcgatgga catggcggtg
    11221 gcgcgcgcca gggcccacgg catcggggtg gtgacggtcg ccaacagcaa ccactacggc
    11281 atcgccgggt actacgtcca gaaggcgatt gacgcgggga tgatcggcct gtcgctcacc
    11341 aatgcggccc ccctcgtggt gccgaccaac ggcgccgacg ccgtcctcgg caccaacccg
    11401 attgccttcg gggccccggg gaaggacggg ttcgacttcc tgctcgacat ggcgaccagc
    11461 gtcgtcccgc gcggcaagct cgaggtgtac gaccgcaacc gccggcagat gccggtggga
    11521 tgggcggtgg acgagaaggg gtacgacacg cgcaacccgg gccacgtgct cgcgaacctg
    11581 ctggcccggt cggggggcgg catcctgccg ctcggcgggc ggggcgagga cttcagcggc
    11641 cacaagggct acggcctcgc gctgatggtg gacgtgctgg cgggcgtcct ctcgggatcg
    11701 gccttcggtg ccgacgtgga caacgtccgc ggcgccccgg gctcgggccc gggctcggcg
    11761 ccgcgggtgg ggcacttctt cctcgcgctc gacatcgcgc gcttcatgcc gctcgaggag
    11821 ttccgcgacc ggctcgccgc cctcttcgcg atgctcaagg actcgcgcaa ggcgctcgac
    11881 aagagcgtga tctacatcca cggcgagaag gagcaggcgc gcacgcgcct gcacgagcag
    11941 tcggggatcc cgctctcgga gaacgtgatg gcgtcgctga ggaagatcgc ggcagactgc
    12001 ggcgccgcgc cgcctatcac cgtcgccgat cggctccgcc gcaaggtgac cgcgcccgag
    12061 gaggggctgt catgagtgcc gcctgggatg tcgcaatcat cggcggaggc atcgtgggaa
    12121 cggccacggc catggccctc gcacgcgacg cgggcgcctc ggtcgtcgtg ctcgaggcgg
    12181 aggacaggct ggcggcgcac cagacgggcc acaacagcgg ggtgattcac tcgggcctct
    12241 actacaagcc ggggtcgctc aaggcccgga actgcgtcga ggggcgcgag gcgatgtacc
    12301 gcttctgcga ggcgcaccag atcccgcacg agcggtgcgg caagctggtg gtcgccacca
    12361 ggaccgaaca cctcgccccg ctcgacgagc tcgagcgccg ggggcgcgcc aacggcctcg
    12421 agggactccg tcgcctggcg ggcgacgaac tgcgcgacta cgagcccgag gtcgccggca
    12481 tcgccgggct gcacgtgccg cagaccggca tcgtcgacta caccgcggtg accgaggcgt
    12541 tcgcccgcct ggtgcgcgag gcggggagcg agatccggac ctcgagccgg gtgctccggg
    12601 tcgagcggcg cccgggagcg gtcgtgctcg agacgcccgg gggggccgtc gaggcgaggg
    12661 cattgatcaa ctgcgcgggg cttcagtccg accgggtggc gcggatgtgc ggcgtggatc
    12721 ctggggtcca gatcgtcccg ttccgcggcg agtactacga gctggtgccc gggcgcgagc
    12781 acctggtccg gaacctgatc tacccggtgc cagacccccg gttcccgttc ctcggcgtcc
    12841 acttcacgcg aatgatccac gggggcgtcg aggcggggcc
//
LOCUS       DAOPQX010000068        12799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5691_length_12799_cov_5.274168, whole genome shotgun sequence.
ACCESSION   DAOPQX010000068 DAOPQX010000000
VERSION     DAOPQX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12799
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5691_length_12799_cov_5.274168"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKK95_05555"
     CDS             complement(<1..669)
                     /locus_tag="PKK95_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOC17711.1"
                     /translation="MEDLLSFIVRLSWVAVLTVLVGLYALGSAVFLILENRKPQSTVA
                     WLFLLLVFPPGGLVIYFLFGRRRHAFSREAELTKLVEGTGLATRSARLLAEQPAKLAA
                     LVESYGEYAQVATTLWSAARAPLTFSNQLEVLQNGREKYPRLLSDIREASHSIHLLYY
                     EWSSDRFTEEVGRLLLEKVRQGVEVRVLYDPVGSLGMLGRRYIRTLRRGGVQMVPFSP
                     LYRLH"
     gene            813..2666
                     /locus_tag="PKK95_05560"
     CDS             813..2666
                     /locus_tag="PKK95_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17712.1"
                     /translation="MRSRLVGTLAVLLAGTAAGTGAAQERATWVRVATPGFEVVAEVS
                     PEQAIDIARRLEAFRSMVEEGLPNPAGALQPALAVVFASDAAFSSFTPSQPGQAAKVA
                     AFVALDESSPCLSFHLERGDESLRTVLHEYARVLLRQTPLWFMEGAAEVYGEARMAGD
                     GRAFRVGDPVALNVLLLRGNFLPLAELLGARRAPSGAAGQAFYAQSWALAHYLLLGSE
                     THARQVAPFLARAAAGTPVVKAFEESFGPLADVERELRRYIERKAWGEKQYALPDAPQ
                     AAPMAPAEVETALGRLLFHAGRDADAEARLERALAADASLPGAHLALGLLRVRQGRIA
                     EALGRLERARALAPESLAPAFHLAKAALRAGEADDETLEAARAGLERLLSPAVRAAEP
                     WAVLGMLSGRLGRLDEAERALRTALDLAPGRLQTIAELADVLAVREKFDEAERLLDSA
                     QEAAARTGTDLGPWRERVAREREVARIRGDLQKAASLGRAEAAPRPRTRGGRTGRWLI
                     PPGYRQTGPGEQRTHGLLRRIDCETGHITVQVATADGTRRFTAKSLAAVQAVSYREGF
                     EPLLACGPRREMEPVFVTWKPAAGAGGGGGARALEGTLVVLEFLGEEYLPR"
     gene            complement(2673..3257)
                     /locus_tag="PKK95_05565"
     CDS             complement(2673..3257)
                     /locus_tag="PKK95_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HOC17713.1"
                     /translation="MKKYEIDPRSALLVVDVQNDFCPGGSLAVPDGDRVIPVLNEAIE
                     HFAAAGLPVFASRDWHPPDTKHFKARGGPWPPHCVAGTEGAAFHPDLRLPADAIVVSK
                     GQDRNDDGYSAFEARTAEGRTVVEELRRRGVTDLYLGGLTTDYCVRATALDARKAGFR
                     VIVLADGIAGIDADDSRRALDEIRAAGGTVVPAA"
     gene            complement(3322..4728)
                     /locus_tag="PKK95_05570"
     CDS             complement(3322..4728)
                     /locus_tag="PKK95_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOC17714.1"
                     /translation="MFEASLQAKRLEKGDLVEGLVVAIGPEVAFVDVGGKGEAAIDIG
                     ELKNDEGELEVAQGDRIQAMVVSTAGGLKLSRKLAGAAATAAQLEDAYQTGLPVEGRV
                     ERAVKGGFEVRIGRQRAFCPISQIDLRGADTSGHEGRVYQFRVIEFKEGGRNVVISRR
                     ALLEVEQQADAARVRESVVPGAVLTGRVTSVRDFGAFVDLGGGVQGLLHVSEMAWSRV
                     ANPAEVVKAGDDITVKVLRVDDDKQRISLGLKQLAPDPWDRVREMYEVGQVRAGRVTR
                     LAEFGAFVELEPGVEALAHASTFAPTGKAGGWSSQVAVGLTGAFEILNIDLDKKRIGV
                     ALLREGWEQALGMTGGKPGIVPGARLTGKVERHEKFGVFVFLAPGRTGLIPNSETGVA
                     NESDVAKTFPVGSDVEVVVLEADEAGRRIRLSARAVTAAREADEVREWSERAGPGASK
                     GFGSIADKLRSALEPRKK"
     gene            complement(4919..5782)
                     /locus_tag="PKK95_05575"
     CDS             complement(4919..5782)
                     /locus_tag="PKK95_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17715.1"
                     /translation="MGFTEELEMAIIGVHGLLYTPEPEALRAMLRDVFGFTFVDAGGG
                     WLIFRLPPAELGVHPAEGPTFESGVRHQLAFMCDDIHQTIADLRARGVAVRGEPEEES
                     YGITVILDLPGVEAMLYEPRHRVAIEQPGHSPVPAAATLLAEALHGPSANAYFLNRGD
                     RGLLGSLEALGAEAASLRPNGRSSIAAHVEHLRYGLNLLNGWARGDDPWPDANWAASW
                     LRQRVSEDEWLHLRRALAEEAHAWLEAVKQPRAWRDPAATEALGSIVHLAYHLGAVRQ
                     LAEAAAGPRAS"
     gene            5925..6458
                     /gene="tadA"
                     /locus_tag="PKK95_05580"
     CDS             5925..6458
                     /gene="tadA"
                     /locus_tag="PKK95_05580"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOC17716.1"
                     /translation="MDEETRGEAGALDHEAGMRLALQQARLGLQAGEVPVGAVVVLGG
                     EVAGAGFNQPIGGHDPTAHAEVVAIRAAAAATGNYRLTGATLYVTVEPCLMCVGAMVH
                     ARIGTVVFGAAEPKSGAILSTVCAPELPGLNHRLHIVGGVLEEECRELIQAFFRERRQ
                     GAEARPVNCLTSPGTVE"
     gene            6474..6564
                     /locus_tag="PKK95_05585"
     tRNA            6474..6564
                     /locus_tag="PKK95_05585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6509..6511,aa:Ser,seq:cga)
     gene            complement(6814..7512)
                     /locus_tag="PKK95_05590"
     CDS             complement(6814..7512)
                     /locus_tag="PKK95_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC17717.1"
                     /translation="MSSAHAGNGRHGRMVTCAEDGLSVFLSLRPRLFGIAYGMLGSAV
                     EAEDIVQDVWLRWQTARRGRVRDATAFLVTTAKRLAINVMRSARSRRETCAGPWLRAP
                     VDKSADPRLQAERGEVLTLGVRILLERLTPNERAAFVLREAFDYAFRDIATILRLREA
                     NARQVVTRARQHLVQGRRRQTSSNEQRRLFEAFIAAAQQGDLVGLEHVFAAAVHVERA
                     PYAYLSSGGTRRSR"
     gene            complement(7966..8805)
                     /locus_tag="PKK95_05595"
     CDS             complement(7966..8805)
                     /locus_tag="PKK95_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC17718.1"
                     /translation="MSAITVKDGTTIYYKDWGKGPVVTFSHGWPLNSDMWDGQMLFLA
                     EHGFRVVAHDRRGHGRSSQPWSGNDMDGYADDLAAVIETLDLEGITMVGHSTGGGEVA
                     RYIGRYGTKRLAKAVLIAAVPPLMLKTAANPEGLPMEVFDGLRSGLVKDRSQTYRDFA
                     PLFYGANRPGAQVSQGTLGQFWLWSMQAGLKNAYDSIKAFSETDFTEDLKKFDIPTLV
                     MHGEDDQVVPVKDSAKKSARLIKGAREIYYPGLPHGLTATHADLVNRDLLAFCQGVKQ
                     AVA"
     gene            complement(9365..9907)
                     /locus_tag="PKK95_05600"
     CDS             complement(9365..9907)
                     /locus_tag="PKK95_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC17719.1"
                     /translation="MSVFTNSASSSPEEIAGYVKAVLGLVEGRDPIGVLRATPQALRD
                     AVAPLDTRELRVPEPGGKWSINEALQHLADSDLVFGWRIRLILAHDRPVITGYDQDLW
                     AGRLHYSEGNPAEALERFAVLRRSNVGLLERASEDDLRRPGLHTERGEETLALVIRLY
                     AGHDLLHLRQIERIRHSIRA"
     gene            10272..11246
                     /locus_tag="PKK95_05605"
     CDS             10272..11246
                     /locus_tag="PKK95_05605"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC17720.1"
                     /translation="MVSDGRAPGIAAAVAVGGQIVWSEGFGYSDLEDKVPASATTLFG
                     IGSISKTLTMAGALSLVDDGLLDLDAPLEAYLPDFPHKGKGITLRRLAAHQSGLTDEF
                     ATSHRQTSRHFRTLEEAYREVIAREVVSYEPGTKVLYGTGLYTIIGRATEVVAKQSFA
                     DLMRERVFQRAGLTTVVPNDRTKKIPARTGFYVERAGGGFEKAPFYDPSHKLPGAGFL
                     ATAADIARFGAALLRPGMLSERARAEIFRAVPLADGTPTEWALGFRVDQFDGRRLVHQ
                     AGGGIGISAWLFIHPEDDLVIAVLSNVSTAPVGGRTHAEIARAFLRQE"
     gene            complement(11338..12468)
                     /locus_tag="PKK95_05610"
     CDS             complement(11338..12468)
                     /locus_tag="PKK95_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HOC17721.1"
                     /translation="MSSTFSLGSAFFQRLYATDVQKMSPSPPSFSSRRKSGVATTGGI
                     VSHTGVAGLTLGGGLGWLMRTHGLTIDNLLAVDVVTANGERLRASEDEHPDLFWALRG
                     GGGNFGVVTSFEFRLHSVGPTVLAGPIVWDASEAVEVLRFYRDFVCHAPDELATIVRF
                     GPAPPLPGIPEDLHWRPVVIVGTCDLGPIRDGDQVLRPLRAFRTPLLDGVRPTQYVDL
                     QGSIDSTVVHGWNYYWKSTHLPELRDDLIDVIVDHAFSSSSPRSYVAMFHLEGAVSRV
                     AESATAFGNRQASHAITLDAVWRPGEDFGERDTAWSRGFFAALGRFRQGVYVNFLNDD
                     EDPGRVREAYGDSTYGQLVEVKTAYDPDNVFHHNHIRPRSGS"
BASE COUNT         2013 a         4369 c         4411 g         2006 t
ORIGIN      
        1 gtgaagccgg tacaagggcg agaacggcac catttgcacg cccccgcggc gcagcgtccg
       61 gatgtagcgg cgtccaagca tcccgaggct gcccaccggg tcgtagagaa cacgaacctc
      121 gacgccctgc cgcaccttct cgagcagcag gcggccgacc tcctcggtga agcggtcgga
      181 cgaccactcg tagtagagca ggtgaatcga gtgcgaggcc tcgcggatgt cggagagcag
      241 ccgagggtac ttctcgcgcc cgttctggag cacctccagc tggttcgaga aggtgagcgg
      301 cgcccgggcc gccgaccaca gcgtcgtcgc cacctgcgcg tactccccgt acgactcgac
      361 gagcgcggca agcttcgccg gctgctctgc cagcagccgc gccgaccgcg tggccaagcc
      421 cgttccctcg acgagcttgg tcagctcggc ctcccggctg aaggcgtggc gtctgcgccc
      481 gaagaggaag tagatcacga ggccgcccgg cgggaagacg agcagcagga agagccaggc
      541 gacggtggac tgcggcttgc ggttctcgag gatcaggaag acggccgagc ccagcgcgta
      601 cagcccgacc agcacggtca ggacggccac ccaggagagg cgaacgatga acgagaggag
      661 gtcctccacg cttggcccgg ctctgcgaac gccaccagca tagctcagcc acaggcctgg
      721 ttcctcgtcg gtcatgcttg gatcggccac gccccctgcg gtagcctcgc gtgcagtggt
      781 agcgcgtgag ttgagcaagg acggaggtga acgtgcgatc gcgcttggtg ggcactctcg
      841 ccgtgctgct cgctggcacc gcggccggta cgggcgcagc gcaagaacgc gcgacgtggg
      901 ttcgcgtggc gacgcccggc ttcgaggtgg ttgccgaggt ctcccccgag caggccatcg
      961 atatcgcgag gcgcctcgag gcgttccgct cgatggtgga agagggcttg cccaacccgg
     1021 ctggagcgct gcagccggcg ctggccgtcg tgttcgccag cgacgccgcc ttttcgtcgt
     1081 tcaccccgag ccagccgggc caggcggcga aggtggcggc gttcgtggcc ctcgacgagt
     1141 cgtctccctg cctgtcgttc cacctcgagc gaggagacga gtcgctgcga acggtgcttc
     1201 acgagtatgc ccgcgtgctg ctgcggcaaa cgcccctctg gttcatggag ggcgcggccg
     1261 aggtgtatgg cgaggcccgg atggccggcg acggccgggc cttccgcgtt ggcgacccag
     1321 tcgcgctgaa cgtgctcctc ctgcgcggaa acttcctgcc gctcgccgag ctcctgggag
     1381 ccaggcgcgc gccctccggt gcggccggtc aggccttcta cgcccagtcg tgggccctcg
     1441 cgcactacct gctcctcggc agcgagacgc acgcccggca ggtcgccccg ttcctggcgc
     1501 gggctgccgc gggcacgccg gtggtgaagg ccttcgagga aagcttcggc cctctcgcgg
     1561 acgtggaacg ggagctgcgg cgatacatcg agcgtaaggc gtggggggaa aagcagtacg
     1621 cgctgcccga cgcgccgcaa gcggcgccca tggcgcctgc cgaagtggag accgcactcg
     1681 gccggctgct cttccacgcc ggccgcgacg ccgatgccga ggcgcgcctg gagcgggcgc
     1741 tcgcggccga tgcgtcactc cccggcgcac accttgcgct cggcctgctt cgcgtcaggc
     1801 agggacgcat cgccgaggct ctcggccgtc tcgagcgggc gcgcgcgctc gcccccgaga
     1861 gcctggctcc agcctttcac ctcgccaagg ccgcgttgcg cgccggcgag gcagacgacg
     1921 agacgctcga ggcggcgcga gccggactgg aaagactgct gtcgcccgca gtgagagccg
     1981 ccgagccgtg ggcggtgctc ggaatgctgt cggggcgcct cggccgcctc gacgaggccg
     2041 agcgcgcgct gcgcacggcg ctcgacctcg cgccggggcg cctgcagacc atcgcggagc
     2101 tggccgacgt cctggccgtg cgcgagaagt tcgacgaggc ggaacgcctg ctcgactcgg
     2161 cgcaagaggc ggctgcccgc acggggaccg acctcggccc gtggcgcgaa cgcgtggcgc
     2221 gcgagcgcga ggtcgccagg atccgcggcg atctgcagaa ggcagcctct ctgggcagag
     2281 ccgaagctgc gccccgcccc cgcacaaggg gaggccggac cgggcgatgg ctcataccgc
     2341 ccggctaccg gcagacgggc cccggcgagc agcgcacgca cggcctgctg cggcgcatcg
     2401 actgcgagac cggacacatc accgtgcagg tggcgaccgc tgacggaacg cggcgcttca
     2461 ccgcgaaatc gctcgcagcc gtccaagccg tcagctaccg ggaggggttc gagccgctgc
     2521 tggcctgcgg accgcgacgc gagatggagc cggtgttcgt cacgtggaag ccagcggcag
     2581 gcgcaggggg cggcgggggc gcacgcgcac tcgagggaac cctggtcgtg cttgaattcc
     2641 tcggagagga atatctgccg cgatagcggt gatcaggcgg cgggcacgac cgtgccgccg
     2701 gccgcccgga tctcgtcgag cgcgcggcgg gagtcgtcgg cgtcgatgcc ggcaatcccg
     2761 tcggcaagga cgatgacgcg gaaccccgcc ttccgggcgt cgagggccgt ggcgcgaacg
     2821 cagtagtcgg tcgtcaggcc tcccagatac aggtcagtga cgccgcggcg gcgcagttcc
     2881 tcgaccactg tccggccctc cgccgtcctc gcctcgaacg cggagtaccc gtcgtcgttc
     2941 cggtcctgtc ccttcgagac gacgatcgcg tccgcaggaa gccgaaggtc gggatggaag
     3001 gccgccccct cggtgccggc cacacagtgc ggcggccacg gaccgccgcg cgccttgaag
     3061 tgcttcgtgt caggcgggtg ccagtcgcgc gaggcgaaga ccggcaggcc ggcggctgcg
     3121 aagtgctcga tcgcctcgtt gagcaccggg atgacccggt cgccgtcggg gacggccagg
     3181 gacccgcccg ggcagaagtc gttctggacg tccaccacca gcagcgcgga gcgggggtcg
     3241 atctcgtact tcttcatcgt tccctcgaag aaccgttgcg acgaacgtgc cgtacgcgaa
     3301 gcgcccggcg cccgttcagc cttacttctt ccggggctcg agcgccgagc gcagcttgtc
     3361 ggcaatcgag ccgaagccct tcgaggcgcc tgggcctgcg cgttcgctcc actcgcgtac
     3421 ctcgtccgcc tcgcgggccg cggtgaccgc cctcgcgctc agccggatgc gccggccggc
     3481 ctcgtcggcc tcgagcacga ccacctcgac gtccgacccg acggggaacg tcttcgcgac
     3541 gtcggactcg ttcgccacgc cggtctcgct gttcgggatg aggcccgtcc ggcccggcgc
     3601 caggaacacg aacactccga acttctcgtg ccgctcgacc ttgccggtga ggcgcgcccc
     3661 gggcacgatg cccggcttgc cgccggtcat gccgagagcc tgctcccagc cctcgcgaag
     3721 cagcgcgacg ccgatccgct tcttgtcgag atcgatgttc aggatctcga aggcgccggt
     3781 gagcccgacg gccacctggc tcgaccaccc gccggctttc cccgtaggcg cgaacgtcga
     3841 cgcgtgggcc agcgcctcga cgccgggctc cagctcgacg aacgcgccga actcggcaag
     3901 ccgggtgacc cggccggcgc gcacctgccc aacctcgtac atctcgcgca cgcggtccca
     3961 cgggtcgggg gcgagctgct tgagcccgag ggagatgcgc tgcttgtcat cgtccacgcg
     4021 cagcaccttg acggtgatgt cgtcgcccgc ctttaccacc tcggcgggat tcgcgacgcg
     4081 cgaccacgcc atctcggaca cgtgcagcag cccctgcacg ccccctccga gatcgacgaa
     4141 cgcaccgaag tcgcggaccg aggtcacgcg gccagtgaga accgcccccg gcacgaccga
     4201 ctccctgacg cgcgccgcgt cggcctgctg ctcgacctcc agcagggcgc gcctggagat
     4261 gacgacgtta cgcccgcctt ccttgaactc gatgacgcgg aactggtaga cgcggccctc
     4321 gtgtcccgag gtgtcggccc cgcggagatc gatctgcgag atcgggcaga aggcgcgctg
     4381 gcggccgatg cgcacctcga agccgccctt cacggcgcgc tcgacccttc cttcgaccgg
     4441 caggcccgtc tggtaggcgt cctcgagctg ggcggcggtc gcggcggcgc cggcaagctt
     4501 ccgcgaaagc ttcaggccgc cggcggtgga caccaccatg gcctggatgc ggtcgccctg
     4561 cgcaacctcg agctcgcctt cgtcgttctt cagctcgccg atgtcgatgg ccgcctcgcc
     4621 cttgccaccg acgtccacga aggccacctc gggcccaatc gccacgacga ggccctcgac
     4681 caggtcgccc ttctcgaggc gcttcgcctg gagagaggcc tcgaacatcg cagcaaaatc
     4741 ttcctcggga tcactcacgg aactcacctc gtccaggggt tcgggattgc tttgcaggag
     4801 gggtacgggc agaccgcctg cggcaccgaa cattctaccg cgggggccgg ccgggccggc
     4861 gccaggcggt tccgctccca gttcactggt atagggttgg cgcttcccca ccccagggtc
     4921 agctcgccct gggcccggct gccgcctcgg caagctgccg taccgcgccg aggtggtagg
     4981 cgaggtggac gatgctgccc agcgcttcgg tggcggccgg gtcgcgccag gcccggggct
     5041 gcttgacggc ttcgagccac gcgtgcgctt cctcggccag cgcccgccgc aagtgcagcc
     5101 actcgtcctc gctgacccgc tggcgcagcc agctggcagc ccagttcgcg tcgggccacg
     5161 gatcgtcgcc gcgcgcccag ccattcagca ggttcaagcc gtagcgcagg tgctcaacgt
     5221 gtgcggcaat cgacgaccgg ccgttcggac gcaagctggc cgcctcggcc ccgagcgctt
     5281 cgagcgaccc gagcagcccg cggtcaccgc ggttgaggaa gtacgcgttg gccgacggcc
     5341 cgtggagcgc ctcggcgagc agcgtggcgg cggcgggaac gggcgagtgt ccgggttgct
     5401 cgatcgccac gcggtgacgc ggctcgtaga gcatggcttc gacgcctggg aggtcgagta
     5461 tgacggtgat gccgtagctc tcttcctcgg gctccccgcg tacggccaca cccctcgccc
     5521 gcagatccgc aatcgtctgg tggatgtcgt cgcacatgaa cgccagctgg tgtcgcacac
     5581 cggattcgaa ggtaggtccc tcggccgggt ggacgcccag ttcggcgggg gggaggcgga
     5641 agatgagcca cccgccgccg gcgtccacga aggtgaagcc gaacacgtcg cggagcatgg
     5701 ctcgcaaggc ctcgggctcc ggcgtgtaga gcaagccgtg cacgccgatg atcgccatct
     5761 cgagctcctc ggtgaatccc actttccatg gtagcgcgcg gcggtcgcgc gggcctcacg
     5821 cgcatgggtc gagtcggcgg gccgggcctc gagggcagcg cgtaggggcg acccttgtgg
     5881 tcgccccgag atgaggcgtg gtccggacgt gccatactcc tcccgtggac gaagagacga
     5941 ggggcgaggc gggggccctg gaccacgaag cggggatgcg gctggctctt cagcaggcgc
     6001 gcctgggcct ccaggccggc gaggtccctg ttggtgccgt ggtcgtgctc ggcggggagg
     6061 tggccggcgc gggcttcaat cagcccatcg gcggccatga tccgaccgcc cacgccgagg
     6121 tggtcgcgat ccgcgcagcg gcagccgcga ccggcaacta ccggctgacc ggggcaaccc
     6181 tctacgtcac cgtcgagccg tgcctcatgt gcgtgggtgc gatggtccat gcccggatcg
     6241 gcacggtcgt cttcggcgcg gccgagccga aaagcggggc catcctctcc acggtgtgcg
     6301 cgccggagct ccccggcctg aaccaccgcc tccacatcgt cggcggcgtg ctcgaggagg
     6361 agtgccgcga gctgatccaa gcgttcttcc gcgagcgccg gcagggggcg gaggccaggc
     6421 ccgtcaattg cctcacctcc ccgggcacgg tagaatgagg tgctaccctg cgcggagagg
     6481 taccgaagtg gtcgtaacgg gggcgcctcg aaagcgtctt gtcgcgtaag cggcacgtgg
     6541 gttcgaatcc caccctctcc gccatccttc gcagaacgct cgattctctc ggctccggtt
     6601 ttctcttcaa ccaacttaac ggggcgtact gggtttctcc cgggatttgc aggcaaggcc
     6661 gctccgactg ccgcctgtcc gcgcttcagc cggctgcacc tcggtcgtac gccttcgcaa
     6721 ccgagtgcgg gccgggctgg cgagccttga gcagcctcta tgctggggct tgaacctaag
     6781 attagtagcg tccggactcg atggtcccga tgctcatctg cttctcctcg tccctcccga
     6841 ggacaggtac gcgtatggcg cccgctcgac gtggacggcg gccgcaaaga catgctcgag
     6901 gccgacgagg tcaccctgtt gggcggcggc aatgaacgct tcgaagagac gcctttgctc
     6961 gttggagctg gtctggcgcc tccttccctg gaccaagtgc tgccgggcgc gagtgaccac
     7021 ttggcgcgca ttggcctccc ggaggcgaag gatggtcgcg atgtcgcgga atgcgtagtc
     7081 gaacgcctcg cgaagaacga acgcggctcg ttcgtttggc gtcagccgtt ccagcagtat
     7141 ccggacaccg agcgtaagca cctcgccgcg ttccgcctgc agccgggggt cggcgctctt
     7201 gtcgactggt gcacgcagcc agggcccggc gcaggtttcc cggcgcgagc gtgcagagcg
     7261 catgacgttg atcgccagtc gcttcgccgt ggtcacgagg aaggctgtgg cgtcacgaac
     7321 ccggccgcga cgggcagtct gccaacgtag ccagacgtct tgaacgatat cctccgcctc
     7381 gacggcgctg cccagcatcc cgtaggcgat cccgaagaga cgaggacgca ggctcaggaa
     7441 gacggaaagg ccgtcctcgg cgcatgtcac catacgcccg tgccggccgt tccccgcatg
     7501 cgcactgctc acgcggggcg gttcagatgg aacaccacgg ccagcggtca tcccgatctc
     7561 ctcttgctgc aatccggccg gtgcgcgtca aagaacaatc agcgcgcgca agatcgacgc
     7621 catccaagaa aggcgtggct ttcgagagaa cgggcgatgg gcttcgtgga ggactgaact
     7681 cgtgtgctgg ccaggacgga ccaacaccgg acacaactga cggaaccccg agtgcccggt
     7741 gcccggtgcg gaggtgcttg cctgaagggc gacgccgata cagaagtgaa agcctggtgt
     7801 ctttgtagta acgggccgcg aggcggcaca gcgagtccgc cgcgccgcgc gcagcgcaag
     7861 cgcgagcggc gccggcaggc gccgggccgc ccgagcagtc tgaagaaacg cgtgattcgc
     7921 ggtgcggcgg ctttcgtcct gacgccgagc tccgcgaggg ccacttcagg cgaccgcctg
     7981 cttgaccccc tgacagaatg caagcaagtc gcggttgacg aggtcggcat gcgtggcggt
     8041 caggccgtgg ggcaggcccg ggtagtagat ctccctggct cccttgatca gccttgccga
     8101 cttcttcgct gaatccttga ccggaacgac ctggtcgtcc tcaccgtgca tgaccagggt
     8161 cgggatgtcg aacttcttca ggtcctcggt gaagtcggtt tcagagaagg ccttgatgct
     8221 gtcgtacgcg ttcttgaggc cggcctgcat gctccacagc cagaactgac ccagcgtgcc
     8281 ctgggacacc tgcgccccag gtctgttggc gccatagaac aggggggcga agtccctgta
     8341 tgtctgcgag cggtccttga ccaggccgct ccgcaggccg tcgaagactt ccattggaag
     8401 tccttccggg ttcgccgcgg tcttcagcat cagcggcggg acggcggcta tgaggaccgc
     8461 ctttgcgaga cgcttcgtcc cgtaccgtcc gatgtagcgc gcgacttctc cgccgccagt
     8521 cgaatggccc accatcgtga tgccctcgag atcgagggtt tcgatcacgg ctgccagatc
     8581 gtcggcatac ccgtccatgt cgttacccga ccaaggctgg ctggatcggc catgtccacg
     8641 acgatcgtgt gcgacgacac ggaagccgtg ctcggcgaga aacaacatct ggccatccca
     8701 catgtccgaa ttcagcggcc atccgtgcga gaaggtgaca acgggcccct tgccccagtc
     8761 cttgtagtag atcgtcgtgc cgtccttcac cgtgatcgcg ctcatgtgcc gtctcctgaa
     8821 ccctgaatga tttgcctgcg aagcgggatt gcctgcaggc ggcgcggccg gcaacagtcg
     8881 tctcacaggg atgccgagaa caccccaaga cctgcctctg ctctcacctc ttcatggcaa
     8941 cggccacgga ggttcgtcgc gtcctcctga gctcgacttg ctcgagggcg gtccctgcgt
     9001 attcgtgaag accggggacc aggacgggat gtgacagccg cagcgaacac cttgaaccgc
     9061 tcggtgcaac tgtcggggcg gccgagtgtc acagcaagcc gcttagggcg gtctgtactg
     9121 acgggtgcgc gcaatgatcc ccgcgaggtt gttgccgact cgcgcgcgag atacttcggg
     9181 gcagaactcg gcgagcgctc actgatccct gccggcaggg cgcaactcgg cgagatccgc
     9241 tttcaggagt ggctgaatca gcccgtcgcc caccgctagc ctgcgcggtg ccgccgcggg
     9301 gcgtcgacaa gggggcctgt ccagggcggg ttcaaggagg tggtcgcgtt cgtccgccaa
     9361 ttcctcaggc gcggatggaa tgccggatgc gctcgatctg gcgcaggtgc agcaggtcgt
     9421 gtcccgcgta cagccttatg accagggcca gcgtttcctc gccgcgttcg gtgtgcagac
     9481 cagggcgccg gaggtcgtcc tcggacgccc gctcgaggag ccccacgttc gaacggcgca
     9541 ggacggcaaa ccgctccagc gcctcggcgg gattgccttc cgaatagtgc agccgtcctg
     9601 cccagagatc ctggtcgtag ccggtgatca cgggccggtc gtgcgcaaga atcagccgga
     9661 ttcgccaccc gaagacaagg tccgagtcag ccaggtgctg cagcgcctcg ttgatcgacc
     9721 acttgccccc gggttcaggc actcgcagct cccgggtgtc gagtggcgcg acggcgtcgc
     9781 gcagagcctg cggcgtggcg cgaagcacgc cgattggatc tcgcccctcg accagcccga
     9841 gtacagcctt cacgtacccc gcgatctctt ctggtgaact cgacgcggag ttggtgaaca
     9901 cggacatggc ggccctcctc ccgctgtctt tgccggtact cccaatagta gccgcacctt
     9961 gcacgctttc cgcgccggaa gagggatact gctttcgtca agcacgccaa gacggcggaa
    10021 ggcacaacat gggaacaagt gggcgtcggg gtgcggggac ggcatctgtc gccgccgccc
    10081 ttggactggg tttgctggcg accttgatcg cctcgcgctc ggctccgctg tctctcgcgg
    10141 cgcctctagc ggagaggccc gcggccggtt cggaacaggc agtggccggc gctcgaggtg
    10201 tcgcccgacc aggtctccag gcggccggcg acgagaaggc gatacatcgc ggaagggaag
    10261 tccttcgtgc cgtggtcagc gacgggcgtg cacccggcat cgcggcggct gtcgccgtcg
    10321 gcggccagat cgtctggtcc gagggattcg gttactccga tctcgaggac aaggtgcccg
    10381 cctcggcaac gacgctcttc gggattggca gcatatccaa gaccctgacg atggccggag
    10441 cgctgtcact cgtggatgac ggcttgctcg acctcgacgc tccgctggaa gcctacctgc
    10501 ccgacttccc tcacaaggga aagggcatca ccctccgccg cctcgcggca caccagagcg
    10561 gcctgaccga cgagttcgcc acttcccacc gccagacatc acggcacttc cggaccctgg
    10621 aggaggctta ccgggaggtg attgcacggg aggtggtctc ctacgaaccc ggtacgaagg
    10681 tgctctacgg gacgggccta tacaccatca tcggccgggc gacggaggtt gtggcgaagc
    10741 agtcgtttgc cgacttgatg agggagcgtg tcttccagcg ggcgggcctg acgacggtcg
    10801 ttcccaacga caggacgaag aagatccccg cgcggaccgg gttctatgtc gagagggccg
    10861 gcggcggctt cgagaaggcg cccttctacg atcccagcca caagctgccc ggggcggggt
    10921 tcctcgcgac tgccgccgac attgcccgtt tcggcgcggc gctgctccgg ccgggcatgt
    10981 tgagcgagcg ggcgcgggcc gagatattcc gggcggttcc gctggcggac ggcaccccga
    11041 ccgagtgggc gctcggtttc agggtggacc agttcgacgg gcggcggctg gtgcaccagg
    11101 ccggcggcgg catcgggatc tccgcatggc tcttcattca cccggaggac gacctggtaa
    11161 tcgcggtgct gtcgaacgtg tccacggcgc ccgtcggcgg gcggacgcac gccgagatcg
    11221 cacgcgcctt cctgcggcag gagtgaccac cagccgtgaa tcggtgtggc cgagagcccc
    11281 gcgtcgcggg ctcagcgtgt gcactggcct gtctcgacgt agctctgcct cgtgcgatca
    11341 agaccccgag cgaggtcgga tgtggttgtg atggaagacg ttgtcggggt cgtatgccgt
    11401 cttcacctct accagctggc cgtacgtcga gtcgccgtac gcttcgcgga cgcggcccgg
    11461 gtcttcgtcg tcgttgagga agttgacgta gacgccttgc cggaagcggc cgagggcggc
    11521 gaagaatccc ctcgaccacg ccgtgtcccg ttcaccgaag tcctcgcccg gccgccacac
    11581 ggcatcgagg gtgatcgcgt gcgacgcctg gcggttccca aacgctgtcg cactctcggc
    11641 cactcggctc accgcccctt ccaggtggaa catcgccacg tacgaccgcg gcgacgagct
    11701 ggagaacgcg tggtcgacga tcacgtcgat caggtcgtcg cgaagctcgg gcaggtgggt
    11761 ggacttccag tagtagttcc agccgtggac gacggtcgag tcgatcgaac cctggaggtc
    11821 tacgtactgc gtgggccgaa ccccgtcaag gagcggagtg cggaatgcgc gaagcgggcg
    11881 aagcacctgg tcaccgtcgc gaatcggtcc caggtcgcag gtcccgacga tcaccaccgg
    11941 gcgccagtgc agatcttccg ggatacccgg caggggcggc gcggggccga atcgcacgat
    12001 ggtcgcgagc tcgtccggag catggcagac gaagtcgcgg tagaagcgaa ggacctcgac
    12061 cgcctcggac gcgtcccaga cgattggccc ggccaggacc gtgggtccga cggagtgcag
    12121 gcgaaattcg aacgaggtga ccacgccgaa gttgccgccg ccgcctcgca acgcccagaa
    12181 cagatctggg tgctcgtcct cggatgcccg cagccgctcg ccattggccg tcacgacgtc
    12241 cacggccaga aggttgtcga tcgtgaggcc gtgcgtgcgc atcagccagc cgaggccgcc
    12301 gccgagcgtg aggccggcca ctccggtgtg gctcacgatg ccgccagtgg tggccacgcc
    12361 gctcttccgc ctggagctga acgagggcgg cgatggcgac atcttctgca catcggttgc
    12421 atacaaccgc tggaagaagg cgcttccgag cgaaaaggtg cttgacacct ttccggtata
    12481 ggagccggtt ttcgcgatcg gcgcgaccaa cgataccccg taaagcgacg acgtcggcgt
    12541 ttgagcaacc ttcaatcgcg acccgcacct ttcagagatc gagatcgaga tcgaagcgca
    12601 ggcgggcgca ggcgcggccg aaacacgtcg aaaaggacga tggtataagg gggcatggac
    12661 atgctcccgc cttcagcacg cggtttcgtc ccctcgtctt tcgagatgtt gtcgttggag
    12721 ctggcacgcc tctccgacaa cgagttgctc gccgaggtcg cacacgtggc gggcgacgag
    12781 cggaccgcga cggcgcgcc
//
LOCUS       DAOPQX010000069        12673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5763_length_12673_cov_4.778650, whole genome shotgun sequence.
ACCESSION   DAOPQX010000069 DAOPQX010000000
VERSION     DAOPQX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12673
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5763_length_12673_cov_4.778650"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PKK95_05615"
     CDS             complement(<1..444)
                     /locus_tag="PKK95_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17722.1"
                     /translation="MQVHQVLATLGYGDAIGNEVLGIRDALRGAGHASDIFVETADPR
                     LEALTRDYRELGDVSSPDTILVHHFSIGSRASRIAFALPDRMVLVYHNITPPEYFLDI
                     HPLLVRQCFRGRRELAAYARRCDLALGDSEFNRQELEAMGFPRTGV"
     gene            complement(459..1778)
                     /locus_tag="PKK95_05620"
     CDS             complement(459..1778)
                     /locus_tag="PKK95_05620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC17723.1"
                     /translation="MKLAVVAQRYGADINGGAELHARYIAERLARHAEVEVLTTCARD
                     YVTWRNELPAGEDRVNGIRVLRFPVRRPRDPDRFGRRSERVFNQTHSDADEHAWLEEE
                     GPAAPGLVKHLARGRTGWDFVVFFSYRYYHAYYGAAAVPDRALLVPTAERDPAAGVSL
                     FGPLFRGVRALMYNSHEERALIEGVSGNTSVPGVVVGVGSEIPERTDAERFRRKHGIT
                     RRFALYVGRIDENKGCKELFDFHRRYARAEPDPLQLVLCGHSLLPIPDTPHVRHLGFV
                     SDEDKFDALAAADLLVMPSYYESLSIVALEAWAVGRPVLANARCDVLKGQCIRSNAGL
                     YYRGYEEFAEALSWLARRRPLAEALGENGRRYFEQNYAWPVIEGKYLHMIERLRREPA
                     VASGEWPPRRPGWRAGRRAVLPPAASVLEQVPAGPVISRREAEPERL"
     gene            complement(1775..2611)
                     /locus_tag="PKK95_05625"
     CDS             complement(1775..2611)
                     /locus_tag="PKK95_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17724.1"
                     /translation="MPDFTVRSDSVDVEEIMGRIRARIREKRGVDYTEEQIRELASVK
                     LERFLDPRKVRSELLQQFREGGISPLPNYSFEDTTLFESKRPWLAFIRRMLRPLLQLF
                     INPNRLVQALHMQASINAHHEQYTNLAYEVLHNLVLETTRLGIEVKNLKMRVESLSSR
                     LDFSERRARALEGVVMYRPGEEPEAEAASAADTPGRANGQGPSELRRKRRRRRSRRPT
                     LAPGAPAGGNPQALAAEDAAGEATEEPIGEHGPGGQPADPRGEEGWPPSRPGSEDGVD
                     EP"
     gene            complement(2628..3581)
                     /locus_tag="PKK95_05630"
     CDS             complement(2628..3581)
                     /locus_tag="PKK95_05630"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOC17725.1"
                     /translation="MTRQPVPETVSRDASVADRAPARSALASLLVSLRPEQWTKNLVV
                     FAGLLFGRQLLDPRAVGLAVAAFAIFCLLSGVVYLLNDVADREADRNHPLKSRRPIAS
                     GELAPGTALAAAALLGAVAFAGAFALGPAFGLVSLAYVALLGAYSARLKHMVIIDVLT
                     IAIGFVLRAVAGAVVIEVPISQWLLVCTVLLALFLALSKRRHEIVLLAGGAPGHRRIL
                     GEYSPYLLDQMIGVVTASTLIAYIFYTLSPETAHRFETPYLGLTTPFPLYGIFRYLYL
                     VHQKEGGGSPSTMLLTDKPLLVCVALWAVSVVVIVYRPWGL"
     gene            complement(3603..3953)
                     /locus_tag="PKK95_05635"
     CDS             complement(3603..3953)
                     /locus_tag="PKK95_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOC17726.1"
                     /translation="MQIHQRQVGDVMIIDVVGKMTLGEGDELLRDKVNSLIQQGHRKL
                     VLNLAEVPYIDSAGLGEIVRTYTTVSRQGGALKLLNLTKRIQDLLSITKLLTVFETYE
                     SEAEAVKSFGPAGV"
     gene            complement(4161..4475)
                     /locus_tag="PKK95_05640"
     CDS             complement(4161..4475)
                     /locus_tag="PKK95_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HOC17727.1"
                     /translation="MEFQDKVLHCVDCGTEFIWTAGEQLFFADKNFKNEPKRCKACKA
                     KRASRPAGTVPARERVETQTTCSECGKVTTVPFKPTQGRPVYCRECFQQRKFAGAGGV
                     GV"
     gene            complement(4498..4695)
                     /gene="rpsU"
                     /locus_tag="PKK95_05645"
     CDS             complement(4498..4695)
                     /gene="rpsU"
                     /locus_tag="PKK95_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOC17728.1"
                     /translation="MAEVKLQEGESVESALRRFKRKVQQEDIIKDIKKHSFYLKPGDK
                     RRAKQALARKRSRKKLRREME"
     gene            4811..6085
                     /gene="purD"
                     /locus_tag="PKK95_05650"
     CDS             4811..6085
                     /gene="purD"
                     /locus_tag="PKK95_05650"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOC17729.1"
                     /translation="MRILVVGGGAREHALAWKLARDPGVTVHCSPGNAGIARVAELAP
                     AGAGTPADFLALAEAIDADLTIVGPEAPLTEGIADLFEARGRLLLGPRRQAARLESSK
                     AFAKTFMARHGVPTAASQVCDTAESARAVLRSGRFGWPVVLKADGLAAGKGVIVADDP
                     LAADLALQALMVDRRFGAAGDRIVIEECMRGAEVSVFALTDGRHALILPTAQDHKRAY
                     DGDLGPNTGGMGAFAPSPLVDDERLEQIRDTIVMPVVEGMREEGQEFRGFLYAGLMLT
                     ADGPKVVEFNVRLGDPEAQVVVPMIDAELAPLLAAAAAGRLGQDTCRVRDWPRVGVVL
                     ASGGYPDAFEKGKVISGLESAETVPGVLVFHAGTTRRDGQVVTSGGRVLTVVGEGHDF
                     PQAISRAYEGASRIAFEGMRMRRDIGRKATLG"
     gene            6132..6599
                     /gene="purE"
                     /locus_tag="PKK95_05655"
     CDS             6132..6599
                     /gene="purE"
                     /locus_tag="PKK95_05655"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOC17730.1"
                     /translation="MGSDSDAPIMQGCVDLLRELGVDCEMTVASAHRSPARVQRLLAD
                     APGRGVQVFVVGAGAAAHLAGMVAAHTARPVIGVPIDSSALKGLDALLSTVQMPPGVP
                     VATVSIGKPGATNAAVLAAQILALGDPALAERLDRYKARLAEKVEQAAARLAK"
     gene            6628..7866
                     /locus_tag="PKK95_05660"
     CDS             6628..7866
                     /locus_tag="PKK95_05660"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOC17731.1"
                     /translation="MNFCVVTFGCRANQADSFRLEEEMCALGHVPVARESADLVVVNT
                     CAVTAAAERSARQLLARIAREQPAARIAVTGCYATRQPDRLERLPRVVALVPNPRKAG
                     LASAIGPAAPAEAGPAVLVPGAGGRTIYPLQVQAGCDERCSYCVVPFTRGPSTSRPLE
                     AVVADARRLAAAGYQEAILTGVHLGAWGRDLAPRRSLGELLAALDVLPGDLRFRLSSL
                     EPMDCTPEVIRQVARRGSRLAPHLHLPLQHASDAVLKAMRRPYTFDDFRRLLEVVRTA
                     LPDAAIGTDVIAGFPGESEADVKRLEQALDELPLSYAHVFAYSDRPGTEASRRRPKVS
                     PREIRERAARLRERADGLRRRFEARQAGTERPALTLGDGTVALTDNYLKVRIPPGLPR
                     NVRVRVRVVGHTPLGGEVVT"
     gene            7863..8459
                     /locus_tag="PKK95_05665"
     CDS             7863..8459
                     /locus_tag="PKK95_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOC17732.1"
                     /translation="MNTLALLGTVFGLAFSAGLNLYATVLVTGLAVRLDWVSLPQNLS
                     SLEALGHPVVLGAAALLYAVEFLADKIPAVDHLWDAAHSVIRPAGAAWIAWRAAGGSG
                     LSEPAEIALLLAAGGIALTTHATKATTRVAVGATGGHALGLGVGLSLLEDVVAFIVSP
                     LAILIPILILLLVVVTLAFAGWVVWRLARRRRLRRQAA"
     gene            complement(8495..10060)
                     /locus_tag="PKK95_05670"
     CDS             complement(8495..10060)
                     /locus_tag="PKK95_05670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOC17733.1"
                     /translation="MNREEMIGRIDQQRSWDFVVIGGGATGAGIAVDAAARGFDVLLL
                     EQSDFGKGTSSRSTKLVHGGVRYLEQGNISLVMEALKERGILRQNAPHLVSNLPFVVP
                     NYDWWEAPFYGVGLKLYNLLAGRYGFGDSEILSPEETLARLPTIKTEGLRGGVVYYDG
                     QFDDSRLLVNLVRTAANQGATLVNYVRVTGFTKAADGTVDGVVARDLESGGEFAASAK
                     VVINAAGPFADAVRRLSDPGAPPLIAPSQGLHLVFDRSFLPGESAIMVPHTRDGRVMF
                     AIPWHGHTVVGTTDTPIPEPLLEPAPFESEVSFILETAAQYLHKAPTRADILSAFVGI
                     RPLVRSGDSRITAALSRDHTIHIDPSGLLTTTGGKWTTYRHMAEDTVNQAVELARLPE
                     RPSVTKTLNIHGFHPHAERFGPLSVYGAEALAILDLSRIDPALGERLDPALPYTRAEA
                     VWAARIEMARTVEDVLARRCRALFLNAAAAARMAPEVAAILARELGRDDRWQREQVDA
                     FSALARRYALLPD"
     gene            complement(10057..11559)
                     /gene="glpK"
                     /locus_tag="PKK95_05675"
     CDS             complement(10057..11559)
                     /gene="glpK"
                     /locus_tag="PKK95_05675"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOC17734.1"
                     /translation="MRYILSLDQGTTSSRAILFDREGAIAAVAQKEFRQIFPRPGWVE
                     HDPQEIWATQFAVAAEAVTRGQASPSDIAAIGITNQRETTVVWDRETGAPVCNAIVWQ
                     DRRTAEFCDRLKAEGHEELVRSRTGLVIDAYFSGSKLAWILEHVPGARERAEAGRLAF
                     GTVDSWLVWKLTGGKAHVTDVSNASRTMLFNIHTLSWDEDLLRLLGVPASLLPEVRSS
                     SEVYGRTSPALGGGDWPIAGMAGDQQAALFGQMCVSPGLTKNTYGTGCFLLQNTGTQP
                     VPSANRLVTTVGWQVGGRTEYALEGSVFIGGAVVQWLRDGLRFFDRSPEVEPLAATVP
                     DNGGVYLVPAFAGLGAPHWDPYARGLLIGLTRGTTSGHIARAALESIAYQVNDLLEAV
                     HGDSGIPMRELRVDGGASTNDALMQFQADLLGVPVVRPAVTETTALGAAYLAGLAVGF
                     WGSIEELARHWKADRRFEPAMARSDAEHLRVRWREAVARAKGWIPAGGGA"
     gene            11639..12451
                     /locus_tag="PKK95_05680"
     CDS             11639..12451
                     /locus_tag="PKK95_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HOC17735.1"
                     /translation="MPTDDTSREALAEFLGTCVLILFGAGVVAQNVLSRGAAGSYLAI
                     NLTWGLGVTMGCYVAGGVSGAHLNPAVTVALAVRRGFPWRKVPIYTAAQIAGAFLAAA
                     IVYLTYREAFAAFDGGTRQVLGELGTGGIFATYPRPFLSTFPGGFIDQVVGTAILMAG
                     IFAISDARNVPPPAGLAPLLVGLLVVAIGAAFGFNSGYAINPARDIGPRLFTAVGGWG
                     IEVFRAGNYWWWVPIVGPCVGAVLGAWAYDVLVGKRIPPIASAEVPPGRLPE"
     gene            complement(12462..>12673)
                     /locus_tag="PKK95_05685"
     CDS             complement(12462..>12673)
                     /locus_tag="PKK95_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17736.1"
                     /translation="EAAGIPYRRPADQAVDHFAQILADHRLTVSVRKSRGRDIRAACG
                     QLIVEGQARSAGQRLAIAMGDGAPR"
BASE COUNT         1743 a         4551 c         4438 g         1941 t
ORIGIN      
        1 cacccctgtc cggggaaacc ccatggcctc cagctcctgc cggttgaatt cggaatcgcc
       61 gagcgccagg tcgcaccgcc gcgcgtaggc cgccagctcg cgccggccgc ggaagcactg
      121 gcgcacgagc agcgggtgga tgtcgaggaa gtactcgggc ggcgtgatgt tgtggtagac
      181 gagcaccatc cggtcgggca gggcgaaggc gatccgcgag gcgcgcgacc cgatcgagaa
      241 gtggtgcacg aggatcgtgt cgggcgagct gacgtcgccg agctcgcggt agtcgcgcgt
      301 gagcgcctcg aggcgcggat cggccgtctc gacgaagatg tccgaggcgt gcccggcgcc
      361 ccgcagcgcg tcgcggatgc cgagcacctc gttcccaatc gcgtcgccgt acccgagcgt
      421 cgccagcacc tggtggacct gcatctcccc cttctttgtc acagccgttc gggttccgcc
      481 tcgcgccggg agatcaccgg gccggcgggc acctgctcga ggaccgacgc cgccggcggg
      541 agcaccgccc ttcgtcctgc ccgccagccc gggcggcggg gcggccactc accggatgcc
      601 acggccggtt cgcgccgcag ccgctcgatc atgtggaggt actttccctc gatcaccggc
      661 caggcgtagt tctgctcgaa gtagcggcgg ccgttctcgc cgagcgcctc ggcgagcggc
      721 cgccggcgcg cgagccacga cagcgcctcg gcgaactcct cgtaaccgcg atagtacaag
      781 ccggcattgc tgcggatgca ctgccccttc agcacgtcgc agcgcgcgtt cgccagcacg
      841 gggcgcccga ccgcccacgc ctcgagcgcc actatcgaga ggctctcgta gtaggacggc
      901 atcacgagca ggtcggcggc ggccagggcg tcgaacttgt cctcgtcgct gacgaacccg
      961 aggtgccgga cgtgcggcgt gtcggggatc ggcagcagcg agtggccgca gagcaccagc
     1021 tgcagcgggt cgggctcggc gcgcgcgtag cgccggtgga agtcgaagag ctccttgcag
     1081 cccttgttct cgtcgatccg gccgacgtac agggcgaagc gccgggtgat gccgtgcttc
     1141 cgccggaagc gctccgcgtc ggtccgctcg gggatctcgg agcccacgcc caccactacc
     1201 ccaggcaccg acgtgttccc cgacacgccc tcgatcagcg cccgctcctc gtgggagttg
     1261 tacatgaggg cccgcacgcc gcggaacagc gggccgaaga gcgacacgcc ggcggcgggg
     1321 tcacgctcgg cggtcggcac cagcagcgcg cggtcgggca ccgccgcggc cccgtagtac
     1381 gcgtggtagt agcggtagct gaagaagacg acgaagtccc agccggtccg cccgcgggcg
     1441 aggtgcttca cgaggcccgg agcggccggg ccctcctcct cgagccacgc gtgctcgtcg
     1501 gcgtccgagt gcgtctggtt gaagacccgt tcggaccgcc ggccgaagcg gtccggatcg
     1561 cgcgggcggc ggaccgggaa ccgcagcacc cggatgccgt tcacccggtc ctccccggcc
     1621 ggcagctcgt tgcgccaggt gacgtagtcg cgggcgcagg tggtgagcac ctccacctcg
     1681 gcgtgccggg ccaggcgctc ggcgatgtac cgcgcgtgca gctcggcccc gccgttgatg
     1741 tcggccccgt accgctgagc gacgacagcc agcttcacgg ctcgtcgacg ccgtcctcgg
     1801 agccgggccg cgaggggggc cacccctcct cgccgcgcgg atccgcgggc tggccgcccg
     1861 gcccgtgctc gccgatcggc tcctcggtcg cctcgcccgc cgcgtcctcg gcggccaggg
     1921 cctgcgggtt ccctccggcc ggagcgcccg gggcaagcgt cggccggcgg cttctccgcc
     1981 gccggcgttt tcggcgcagc tccgacggcc cctgcccgtt ggcgcggccc ggcgtgtcgg
     2041 cggcggaggc cgcctcggcc tccggttcct cgcccggccg gtacatcacg acgccctcga
     2101 gggcgcgggc gcggcgctcg ctgaagtcga ggcggctcga gagcgactcg acgcgcatct
     2161 tcaggttctt cacctcgatg ccgaggcgcg tcgtctcgag gacgaggttg tggagcacct
     2221 cgtaggcgag gttggtgtac tgctcgtggt gggcgttgat gctggcctgc atgtgcagcg
     2281 cctgcaccag ccggttcggg ttgatgaaca gctgcagcag cggcctgagc atccggcgga
     2341 tgaaggccag ccagggccgt ttcgactcga agagcgtcgt gtcctcgaac gagtagttcg
     2401 gcaggggcga gatgcccccc tcgcgaaact gctgcagcag ctccgaccgg accttgcgcg
     2461 ggtcgaggaa gcgctcgagc ttgacgctgg ccagctcgcg gatctgctcc tcggtgtagt
     2521 ccacgccgcg cttctcgcgg atgcgggcgc ggatccggcc catgatctcc tcgacgtcga
     2581 ccgagtcgga gcggacagtg aaatcaggca tggcttgagg gtgcgcctca gagcccccac
     2641 gggcggtaca cgatcaccac gaccgacacg gcccacagcg ccacgcacac gagcagcggc
     2701 ttgtcggtga gcagcatcgt cgaggggctc ccgccgccct ccttctggtg cacgaggtag
     2761 aggtagcgga agatcccgta gagcgggaag ggcgtggtga ggccgaggta cggcgtttcg
     2821 aacctgtggg cggtctcggg gctcaaggtg tagaagatgt acgcgatcag cgtggacgcg
     2881 gtgaccacgc cgatcatctg gtccagcagg tacggcgagt actcgccaag gatccggcgg
     2941 tgaccggggg cgccgccggc caggagcacg atctcgtggc ggcgcttgct gagcgccagg
     3001 aagagcgcga gcagcaccgt gcagacgagc agccactggc tgatcggcac ctcgatcacc
     3061 accgcgccgg cgaccgcgcg cagcacgaag ccgatcgcga tggtgaggac gtcgatgatc
     3121 accatgtgct tcaggcgcgc ggagtacgcg ccgagcagcg ccacgtacgc cagtgacacc
     3181 aggccgaagg cggggcccag cgcgaacgcc cccgcgaagg cgaccgcgcc gagcagcgcc
     3241 gccgccgcga gcgccgtccc gggcgccagc tcacccgagg cgatcggccg gcgcgacttc
     3301 agcgggtggt tgcggtcggc ttcccggtcg gcgacgtcgt tcaacaggta cacgacgccc
     3361 gagagcaggc agaagatggc gaatgcggcg accgccaggc cgacggcgcg cggatcgagc
     3421 agctgccggc cgaagagaag cccggcgaag acgaccaggt tcttcgtcca ctgctcgggg
     3481 cgcagcgaaa ccagcaggct ggccagcgcg gatcgggccg gggcgcggtc cgccacggac
     3541 gcgtcccggc tcacggtttc cggcaccggt tggcgcgtca tgacgcgcgg ttccgggacg
     3601 tctcaaacac cggccgggcc gaagctcttc accgcctcgg cttccgactc gtaggtctcg
     3661 aataccgtca gcagcttggt gatcgacagc aggtcctgga tccgcttggt caggttcagc
     3721 agcttcaggg cgcccccctg gcggctcacg gtcgtgtagg tccgcacgat ctcgcccagg
     3781 ccggcgctgt cgatgtaggg gacctcggcg aggttgagga cgagcttgcg gtgcccctgc
     3841 tggatcaggc tgttcacctt gtcgcgaagg agttcgtcgc cttcgccgag cgtcatcttg
     3901 ccgacgacat cgatgatcat cacgtcgccg acctgtctct ggtggatctg cattccgtct
     3961 cctccagcct cacgggccca gcgcccctcc gttaccttcg ctcaaccggt agtatgaggc
     4021 atgtcatctt agcgtagcgc tgaaaaatgc gtcaacagac cggcgcgcgc gatcccggga
     4081 acggtctctc cgcccccgta cacgaaaagg ggggccccgc ttgcgcgggc cccccttcga
     4141 gtcgccctct cgtggagtcc ctataccccg acgccgccag cacccgcgaa cttcctctgc
     4201 tggaagcatt cgcggcagta caccggacga ccctgcgtcg gcttgaacgg gaccgtcgtg
     4261 accttcccgc actccgagca ggtcgtctgc gtctcgacgc gctcacgggc cgggaccgta
     4321 ccggccggcc tgctggcccg cttcgccttg caggccttgc accgcttggg ctcgttcttg
     4381 aagttcttgt cggcgaagaa cagctgttcc cccgcggtcc agatgaactc cgtcccgcaa
     4441 tccacgcagt gcaggacctt gtcttggaac tccatcggtg gttccccccg aatctcgcta
     4501 ctccatctca cgccggagct tcttgcgact ccgcttgcgc gcgagggcct gcttggcccg
     4561 ccgcttgtcc cccggcttca aatagaagga gtgcttcttg atatccttga tgatatcttc
     4621 ctgctgaacc ttccgcttga agcggcgaag ggcgctctcg accgactcgc cttcctgcag
     4681 cttgacttca gccacgcccg tgacaccccc tttactggcc cggctggctc ctggggcttc
     4741 cctctgaaat gcccgtaatg tgctaggcta tcacgaacag cgggcagttt tcggccgaaa
     4801 ttttacgacc atgcggatcc tcgtcgtcgg cggcggcgca cgcgaacacg ccctcgcctg
     4861 gaaactcgcc cgcgacccgg gcgtcaccgt gcactgctcg ccggggaacg ccggcatcgc
     4921 gcgggtggcc gagttggcgc ccgcgggcgc cggcaccccc gccgacttcc tcgcgctcgc
     4981 cgaggccatc gacgccgacc tgaccatcgt cggccccgag gcgccgctca ccgaggggat
     5041 tgccgatctc ttcgaggctc gcggccggct gctgctcggg ccgcgccgcc aggccgcccg
     5101 cctcgaatcg agcaaggcgt tcgcgaagac cttcatggcc cggcacggcg tcccgaccgc
     5161 cgccagccag gtgtgcgaca cggccgagtc ggcacgcgcc gtcctccggt cgggccgctt
     5221 cggctggccg gtcgtgctca aagccgacgg cctcgccgcc ggcaagggcg tgatcgtcgc
     5281 cgacgacccc ctcgccgccg acctcgccct gcaggccttg atggtggacc ggcgcttcgg
     5341 tgcggccggc gaccggatcg tgatcgagga gtgcatgcgc ggggccgagg tctcggtctt
     5401 cgccctgacc gacggccggc acgccctgat cctgccgacg gcgcaggatc acaagcgggc
     5461 gtacgacggc gacctcgggc cgaacactgg gggcatggga gccttcgcgc caagcccgct
     5521 ggtggacgac gagcggctcg agcagatccg tgacaccatc gtcatgccgg tcgtcgaggg
     5581 catgcgggag gaagggcagg aattccgggg cttcctctac gccggcctga tgctgacggc
     5641 cgacgggccg aaggtggtgg agttcaacgt ccgcctgggc gaccccgagg cccaggtggt
     5701 ggtgccgatg atcgacgccg agctggcgcc gctgctcgcc gccgccgccg ccgggcggct
     5761 cggccaggac acctgccgcg tgcgcgactg gccgcgcgtg ggagtggtgc tggcctcggg
     5821 cggctacccc gacgccttcg agaagggcaa ggtcatctcc ggcctcgaga gcgccgagac
     5881 ggttcccggg gtgctcgtgt tccatgccgg caccacccgg cgcgacgggc aggtcgtcac
     5941 ctccggcggc cgcgtgctga cggtggtcgg cgaggggcac gacttccccc aggccatctc
     6001 gcgcgcctac gagggagcct cgcgcatcgc gttcgagggg atgcggatgc ggcgggacat
     6061 cgggaggaag gcgacccttg gatagggcag gtgccatgga acaggccgac cggatctccg
     6121 ttctcgtcct gatgggttcc gattccgacg cgccaatcat gcaggggtgc gtcgaccttc
     6181 tgcgggagct gggcgtcgat tgcgagatga ccgtcgcctc ggcccaccgg tcgccggccc
     6241 gcgtgcagcg gctgctggcc gacgcgccgg gccgcggtgt gcaagtgttc gtcgtcggcg
     6301 cgggggcggc cgcccatctt gcgggcatgg tggcagccca caccgcacga ccggtaatcg
     6361 gggtgccgat cgattcctcg gcgctcaagg ggctcgacgc gttgctttcc accgtgcaga
     6421 tgccgcctgg cgttcccgta gccaccgtgt cgattggcaa gccgggagcg accaacgcgg
     6481 cggttctcgc cgcacagatc ctggcgctgg gcgacccggc actcgccgag cggctcgacc
     6541 ggtacaaggc aaggctcgcc gagaaggtcg agcaggccgc cgccaggctc gcgaagtagc
     6601 accggcctcc gtgccggcaa ttggaacttg aacttctgtg tcgtcacctt cggctgccgc
     6661 gccaaccagg ccgactcgtt ccgcctggaa gaggagatgt gcgccctcgg tcacgttccg
     6721 gttgcgcgcg agtcggccga cctcgtggtg gtgaatacgt gcgcagtcac cgccgcggcc
     6781 gagcgctcgg cgcgccagct cctggcgcgc attgcccgtg agcagccggc cgcgcgcatc
     6841 gcggtgaccg gctgctacgc caccaggcag cccgatcggc tcgagcggct tccccgtgtc
     6901 gttgcgctcg tgcccaaccc ccggaaggca gggctggcat cggcgatcgg ccccgccgca
     6961 ccggctgagg ccgggccggc cgtgctcgtc cccggggccg gtggaagaac catctacccg
     7021 ctgcaggtcc aggccggctg cgacgagcgc tgcagttact gcgtggtgcc gtttacgcgc
     7081 ggcccgagca cgagccggcc cctcgaggcg gttgtggccg acgcgcgaag gcttgccgcg
     7141 gccggctacc aggaggcgat cctcaccggc gtgcacctgg gggcctgggg gcgcgacctc
     7201 gcgccgcgcc gctcgctcgg tgaactgctc gcggctctcg acgtgcttcc tggcgacctg
     7261 cgcttccgcc tcagctcgct cgagccaatg gactgcacgc cagaggtcat tcgccaggtg
     7321 gcgcggcgcg ggagccggct cgcgccgcac cttcaccttc cgctccagca cgccagcgac
     7381 gcggtactga aagcgatgcg gcgtccttac acgttcgacg acttccggcg cctgctcgag
     7441 gtggtccgca cggccctccc cgacgcggcg atcgggaccg acgtgatagc aggcttcccg
     7501 ggcgagagcg aggcggacgt gaagcggctc gagcaggcgc tcgatgagct tccactctct
     7561 tacgctcacg tcttcgccta ctccgaccgc cccggcaccg aggcctcccg ccgccgtccg
     7621 aaggtgtctc cgcgcgagat ccgcgagcgg gcggcccggc tgcgcgagcg cgcggacggg
     7681 ctgcggcgcc ggttcgaggc ccggcaggcg ggaaccgagc ggccggcgct gacccttggc
     7741 gatggcacgg tcgcgctgac cgacaactac ctcaaggtgc ggattcctcc tgggctcccg
     7801 cgcaacgtga gagttcgcgt gcgcgtagta ggtcatacgc cgctgggcgg ggaggtcgtc
     7861 acgtgaacac gctcgcgctg ctcggcaccg tcttcggcct ggcgttctcg gcgggcctga
     7921 acctctacgc aaccgtcctg gtgacagggc tggcggtgcg cctcgactgg gtcagcctcc
     7981 cacagaacct gtcgagcctc gaggcgctcg gccaccccgt cgtgctcggg gcggccgccc
     8041 tgctttacgc ggtggagttc ctcgccgaca agattcctgc agtcgatcac ctctgggatg
     8101 cggcgcacag cgtgatccgc ccggccggag cggcctggat cgcctggcgc gcggcgggag
     8161 gcagcggcct ctccgagccg gccgagatcg cgctcctgct ggccgccggc ggcatcgccc
     8221 tgacgacgca cgccaccaag gcgactaccc gcgtcgcggt gggggcgacg ggcggccacg
     8281 cgttgggcct cggggtcggc ctcagcctcc tcgaggacgt cgtggccttc atcgtctcgc
     8341 cgctcgcgat cctgatccca atcctcatcc tgctgttggt cgtggtcacg cttgccttcg
     8401 cgggctgggt cgtctggcgc ctcgcgcggc ggcggcgcct gcggcgccag gcggcctgag
     8461 ccgatcgcca ggcgggcgag gcgttcccaa tcgtctaatc gggcagcagg gcgtagcggc
     8521 gcgccagcgc gctgaacgcg tcgacctgct cgcgctgcca gcggtcgtcc cggccgagtt
     8581 cacgcgccag gatggccgcc acctccggcg ccatgcgcgc cgcggccgcc gcgttcagga
     8641 agagcgcccg gcagcggcgc gccagcacgt cctccaccgt tctcgccatc tcgatccggg
     8701 ccgcccacac ggcctcggcc ctcgtgtagg gcaacgccgg gtcgagccgt tcgcccagcg
     8761 ccgggtcaat ccgcgacagg tcgaggatcg cgagcgcctc ggcgccgtac acgctcagcg
     8821 gtccgaagcg ctcggcgtgc gggtggaagc cgtggatgtt cagcgtcttg gtcaccgacg
     8881 gccgctcggg cagccgcgcc agctcgacgg cctggttgac cgtgtcctct gccatgtgcc
     8941 ggtaggtcgt ccacttgccc ccggtcgtgg tcagcagtcc cgacgggtcg atgtgaatgg
     9001 tgtggtcgcg cgagagggcg gcggtgatgc ggctgtcgcc gctccgcacc agcggccgga
     9061 tgccgacgaa cgcgctcagg atgtcggccc gcgtcggggc cttgtggagg tactgcgccg
     9121 cggtctcgag gatgaaggag acctccgact cgaacggcgc cggctccagg agtggttcgg
     9181 ggatcggggt gtcggtcgtg ccgaccacgg tgtggccgtg ccacgggatc gcgaacatca
     9241 cccgtccgtc gcgggtgtgg ggcaccatga tcgcactctc gcccggcagg aacgaccgat
     9301 cgaagacgag gtggagtccc tggctcggcg caatcagagg tggcgcgcca ggatccgaga
     9361 gccggcgcac ggcgtcggcg aatgggcctg ccgcgttgat caccaccttg gccgacgccg
     9421 cgaactcgcc gccgctctcg aggtcgcggg ccaccacgcc gtccaccgtg ccgtcggcgg
     9481 ccttggtgaa cccggtgacc cggacgtagt tgaccagcgt ggctccctgg ttcgccgcgg
     9541 tgcggaccag gttgacgagc aggcgcgagt cgtcgaactg cccgtcgtag tacaccacgc
     9601 cgccgcgcag cccttcggtc ttgatcgtcg ggaggcgggc gagcgtttcc tcgggcgaaa
     9661 ggatttccga gtcgccgaag ccgtaccggc ccgcgagcag gttgtagagc ttgagcccca
     9721 cgccgtagaa cggcgcttcc caccagtcgt agttcggaac gacgaatggc aggttcgaga
     9781 ccaggtgcgg ggcgttctgc cgcaggatgc ccctctcctt caacgcctcc atcacgagcg
     9841 agatgtttcc ctgctcgagg taccggaccc cgccgtgcac gagcttcgtg ctgcggctcg
     9901 acgtcccctt gccgaagtcg ctctgctcga gcagcagcac gtcgaacccg cgggcggccg
     9961 catcgactgc aatcccggcc ccggtcgccc cgccgccgat gacgacgaag tcccaggagc
    10021 gctgctgatc gatccggccg atcatttctt cgcggttcac gcgccacctc cggccgggat
    10081 ccagcccttc gccctggcaa ccgcctcgcg ccagcggaca cgcagatgct cggcgtcgct
    10141 gcgcgccatc gcgggctcga agcgccggtc cgccttccag tgccgcgcga gttcttcgat
    10201 cgagccccag aagcccacgg caaggcccgc caggtacgcc gcgccgagcg cggtggtctc
    10261 ggtgaccgcc ggccgcacga ccggcacgcc aagcaggtcg gcctggaact gcatgagcgc
    10321 gtcgttggtc gaggcgcccc cgtcgacgcg caactcgcgc atcgggatcc ccgagtcgcc
    10381 gtgcacggcc tcgagcaggt cgttcacctg gtaggcgatg ctctcgagcg cagcccgggc
    10441 gatgtggccg ctcgtggtgc cgcgcgtcag gccgatgagc agccctcgcg cgtaggggtc
    10501 ccagtgcggg gcgccgagcc cggcgaacgc ggggacgagg tagacgcccc cgttgtcggg
    10561 caccgtggct gcgagaggct ctacctcggg cgagcggtcg aagaagcgaa ggccgtcgcg
    10621 cagccactgc accaccgccc caccgatgaa gacgctgccc tcgagcgcgt actcagtgcg
    10681 gccgccgacc tgccagccca cggtggtgac gagccggttg gccgacggga ccggctgggt
    10741 gcccgtgttc tgcagcagga aacagcccgt cccgtaggtg ttcttcgtga gccccggcga
    10801 cacgcacatc tgcccgaaca gcgccgcctg ctggtcaccc gccatccccg cgatcggcca
    10861 gtcgccgccg ccgagcgcag gggacgtccg cccgtagacc tcgctcgacg agcggacctc
    10921 ggggagcagg ctggccggga ccccgagcag ccgcagcagg tcctcgtccc acgacagcgt
    10981 gtggatgttg aacagcatgg tgcgcgaggc gttgctgacg tcggtgacgt gcgccttccc
    11041 gcccgtcagc ttccagacca gccaggagtc gacggtgccg aacgccaggc gccccgcctc
    11101 ggcccgttcc cgggcccccg gcacgtgctc gaggatccag gccagcttgc tgcccgagaa
    11161 atacgcgtcg atgaccaggc cggtccgcga ccgcaccagc tcctcgtgcc cctcggcctt
    11221 caggcgatcg cagaactcgg ccgtccggcg gtcctgccag acaatcgcgt tgcagacggg
    11281 tgccccggtc tcgcggtccc agaccacggt ggtctcgcgc tggttggtga tgccgatggc
    11341 cgcgatgtcg gagggcgacg cctggcccct cgtgaccgcc tcggccgcca ccgcgaactg
    11401 tgtggcccag atctcctgcg ggtcgtgttc gacccacccg ggcctcggga agatttgccg
    11461 gaactccttc tgggccaccg ccgcgatcgc gccctcgcga tcgaacagga tggcccgcga
    11521 gctggttgtg ccctggtcga gcgacaggat gtagcgcatc ccgggagtat acttcgccat
    11581 ccgaccgcgc tcgggctggc agcgccgggc ggccccccgc atcgacatcg aggagcctat
    11641 gcccaccgac gacacctccc gcgaagccct cgccgagttc ctcggcacct gcgtgctcat
    11701 cctgttcggc gccggcgtcg tggcgcagaa cgtgctgagc cgcggcgcgg ccggcagcta
    11761 cctggccatc aacctcacct ggggcctcgg cgtcaccatg gggtgctacg tcgccggcgg
    11821 cgtctcgggt gcccacttga accccgccgt cacggtggcg ctcgcggtca ggcgcgggtt
    11881 cccctggcgc aaggtgccga tctacacggc ggcccagatt gcgggcgcct tccttgccgc
    11941 cgcgatcgtc tacctgacct accgggaggc gttcgcggcc ttcgacggcg gcacccggca
    12001 ggtgctcggg gagctgggca ccggcgggat cttcgccacc tacccccgcc cgttcctgag
    12061 cacctttccc ggtggattca tcgaccaggt cgtcggtacg gccatcctga tggccggcat
    12121 cttcgcgatc tccgacgcgc gcaacgttcc cccgccggcc gggctcgcgc cgctgctggt
    12181 cgggctgctg gtggtggcca tcggggccgc gttcggcttc aactcgggtt acgcgatcaa
    12241 cccggcacgc gacatcggcc cgcggttgtt cactgccgtt gggggatggg gcatcgaggt
    12301 cttccgcgcg gggaactact ggtggtgggt gcccatcgtc ggcccgtgcg tgggcgccgt
    12361 gctcggcgcg tgggcttacg acgtgctggt ggggaagcgc atcccgccaa tcgccagtgc
    12421 ggaagttccg ccggggcgcc ttccggagta gccgccgacg gtcatcgcgg ggcgccgtct
    12481 cccatcgcga tcgcgaggcg ctggccggcc gaacgcgcct ggccctcgac gatcagctgg
    12541 ccgcaggccg ccctgatgtc acggccccgg ctcttcctga ccgagacggt gaggcggtgg
    12601 tcggcgagga tctgcgcgaa gtggtcgacg gcctggtctg ctggccggcg gtaggggatg
    12661 ccggccgcct cgt
//
LOCUS       DAOPQX010000070        12630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5790_length_12630_cov_4.736700, whole genome shotgun sequence.
ACCESSION   DAOPQX010000070 DAOPQX010000000
VERSION     DAOPQX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12630
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5790_length_12630_cov_4.736700"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PKK95_05690"
     CDS             complement(<1..1448)
                     /locus_tag="PKK95_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOC17737.1"
                     /translation="MNAVVVGAGAAGGVVAKELATAGLSVVLLERGRWQTFDDHDHDE
                     LISQRTTVLGNAFGPDDERHRRVVRLGDRFQTVLPSEGAYGNNAGCVGGGTLSYGAMA
                     WRFMEQDFRMRSIYGCPEGSTLEDWPITYHDLEPFYEKAEQEIGVAGDADANPFQAPR
                     RKPYPMPPFAYNTEGRLLEASARRLGFHPFPLPMLRNSVPRDGRAACIHCRSCVGFAC
                     EVQAKCGTQNTVIPAAIRTGNCRVFTGCVAKEVLTDARGRATGVAYFDPRDRLQRQEA
                     DLVVISASATESARLLLNSKSRLFPAGLGNRYDWVGRNLQGHAYSGAYGLFEQETYDD
                     VGAGATIGICDFNHGNPGIRGGAMLANEFIRLPYLFTNVRPPGSARWGRGHKEFQRKY
                     YRRSVGVQGPIQEMPVFESRVEVDPVVKDHWGIPVARISGSRHPHDVEIGQFMAEKAE
                     LWLKETGAGQTWRRVAGRGVSGSGVRRRSRFE"
     gene            complement(1524..2069)
                     /locus_tag="PKK95_05695"
     CDS             complement(1524..2069)
                     /locus_tag="PKK95_05695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HOC17738.1"
                     /translation="MQDPLSRRQFILVSALSAAASAFWSACARFQPAPAFTPEERRLV
                     EALADQVIPPDDVPGGKDAGVAEFIDRQLRGPYRRHRQAYRSGLVKIDETSRRTAGAP
                     FAELAFDRQTALLQALEADRVPEGIWAPGEAREFFRLVADHCLQGYYGSPRHGGNRNF
                     ASWKMLGLDTPQVLGRVVVKG"
     gene            complement(2200..4314)
                     /locus_tag="PKK95_05700"
     CDS             complement(2200..4314)
                     /locus_tag="PKK95_05700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC17739.1"
                     /translation="MRAVLILGSGLALLHLAQLPSASPSARGPAAGDPVPVRAQAAMR
                     DLATSDLYRLRSVGDVRLSPDGRRIAYSVTSADSPGRPRSRAWITDLDTGRSTPLGNE
                     SSNGRWSPDGSLVAWLDRGDDAGEGAGLMVSQPDGSDARLLVATSGTNHPLPGEGERI
                     SWSPDGTRIAFVSSTPGPETDEASGDPMVITRYLYKPTASEGMTRFNDNRRLHVFVAD
                     LAAHPGQRVRQLTDGPYYEHSIAWSPRGDEILFVSNREPDPDRVFNNDLFAVDVADRT
                     VRRLTDTRSAEYQPAWSPDGRTIAFLGTTRPLTSSETTMEDTHVWVMDAEGRNRREAG
                     AAIDNRQERPAWSPEGDALYFTVEERGSVRLYRLPVSGGAPSPVAPDPTDAGAVSSFS
                     VGRGRTVAFSMATRSGPAELFRRQQDWTPRQLTSLNRPLLAGRTLALVESIQYGSGDL
                     TPIEAFLTRPVRVDPGGRHPLIAMIKGGPHAQQGPVFNAKAQIYAAHGFAVLMVNYRG
                     STGYGQAFADAIFKDQNGGEANDVLAGVDAALAKYSWLDPQRLGIEGVSYGGQLTNWL
                     ITRTPRFKAAIPTAGISNLVTQNYLAYYHDYLAVEFGGYPHEQWQSDAGAKPRLLMDV
                     LWERSPLRHVANVKTPVLLLHGENDSDVPIAEAEQFYIALKDAGVETVMVRYPREGHG
                     LRETVHVIDSIERSIAWYEAHW"
     gene            4512..5258
                     /locus_tag="PKK95_05705"
     CDS             4512..5258
                     /locus_tag="PKK95_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOC17740.1"
                     /translation="MNIRRFPTSTELADAAAAQATEALTRAIAARGRARIVAATGSAQ
                     FEFLSVLTRTPGIDWGRVEMFHLDEYVGLSADHPASFRRFLLERLIRPAGIGTYHLLD
                     GDTGDPEAVCERVGRELQAAPVDVTFMGIGENGHIAFNDPPADFETERPYIVVELDEV
                     CRRQQVGEGWFASLAEVPTRALSMSVKQLLRSDELICIVPQSRKAKAVQAALEGPITP
                     MVPASILRTHPNVTLYLDAESSSLLREARS"
     gene            5568..6314
                     /locus_tag="PKK95_05710"
     CDS             5568..6314
                     /locus_tag="PKK95_05710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC17741.1"
                     /translation="MAFQDHYSLLAARYARARPGYPDALFDYLASVAPGHGLAWDCGT
                     GNGQAARGLARRFACVHATDASAEQLRHAEPHPRVSYHREPAERVSLRDESVDLVTSA
                     VAAHWFDLDRFYPEVRRVLKPGGAVALWTYHLPRVSPAVDRLVAYYYSTLLAGHWPDR
                     ISYVDQRYATLPFPFDELEPPALTMTTSWTAAQLAAFLASWSAVPRYQKAHGRHPVDE
                     IWDDLVTAWEGDDATRTITWDLHLRVGLSR"
     gene            complement(6337..7242)
                     /locus_tag="PKK95_05715"
     CDS             complement(6337..7242)
                     /locus_tag="PKK95_05715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC17742.1"
                     /translation="MEQQLPQRSLGRTGARVSIVGVGGFHLGGIEEAEAVRIIRTAVD
                     HGMTFLDNSWDYHGGASEERMGRALAGGYRERAFLMTKIDGRTWESANAQLEESLLRL
                     RTDRVDLLQFHEIIRMEDADRIFAPGGALEAVVAAREAGKLRFIGFTGHKSPEILLHM
                     LSVADAHGFTFDAVQLPLNPMDAHVESFEKMVLPVLEARGIGVLGMKPLGAGKLLESD
                     TVSAEECLRYALSLPTSTVITGCDSMKVLDQAVRVARAFSPYTADEMNALRERTRQPA
                     LGRAFEPYKTTAEHDSTAQNPRWLG"
     gene            complement(7574..9076)
                     /locus_tag="PKK95_05720"
     CDS             complement(7574..9076)
                     /locus_tag="PKK95_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOC17743.1"
                     /translation="MQHSSRDVSRDLPSLVRGCTFDDFLFAPQFSVLERRDPAVIDLS
                     TRLSEHLVLRRPIVSANMDTVTRAAMAIALAEEGGIGIIDRGFRPGEIWPQVREVEIV
                     KRTQHGVIPDPYTIAPHATLAEAADAMRRSGVGTLVVVDAQNRLEGLLTERDTRFVEG
                     DVKVAERMTPFDRLVVHEGAIALDEAERIMVERKVKKLPLVDAGRRLLGLVTAKDILK
                     QKRLPFATRDAQGRLRVGAAIGATGDYLERAAGLVAAGTDLIVIDIAHGHSVVMARAL
                     EEFRKRFPDVELVAGNVATAEGARFLAERGANGIKVGIGPGGGCTTRLTTSFGVPQVQ
                     ALVECRMATRLPIIADGGVRRDGALVQALLFGGDTVMLGSAFAGTREAPGEVVRKAVV
                     VPESQKSVKIPFKVLRGMASIEAIKDRLDVEDAAAADLEAIGAEGFEVAVPERGSVRP
                     IVRDMIKHLCSAISYGGAASLPELRERFLADPSRYVIRLSRAARRESYER"
     gene            complement(9206..9628)
                     /locus_tag="PKK95_05725"
     CDS             complement(9206..9628)
                     /locus_tag="PKK95_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17744.1"
                     /translation="MSSVRRPTSLTTSAILPLLAFIAAVALAGCGADSPTAPGGGVVA
                     RILVSGETFRVLLTTDEQVAAAKAAQAGGRASIPHGRIVAGTQVNAGYSWHLEDVRFV
                     EVTIEVCDGRPSMVEAAGGPSFADGTFCPWGARVISVE"
     gene            complement(9778..>12630)
                     /locus_tag="PKK95_05730"
     CDS             complement(9778..>12630)
                     /locus_tag="PKK95_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC17745.1"
                     /translation="LKVDDYFALKDVRDPRLSPDGKWVAWTVTSMDLKKDTSDSDIWM
                     APVAGGEAVRLTTSDKPERAPRFSPDGKWLAFLSGRAGSHAQVWLLPLSGGEAFKLTS
                     YKAGVSDLAWSPDSTRLALVVDDVDPDAPADEEEEGKGDDKKAKKPIVVKRLQFKRDY
                     QGYLTDLRSHIHVFDVASKSSRQLTSGPFDDGDPAWSPDGTLIAFSSNRTLPDPDRNQ
                     NSDIYVVPAKGGIPRPLVNTDRAESNPVFSPDGRRLAFVSSGDPNDMWYGATHIGIVE
                     VPGEWNGSGAAQATAQATAKATALTAPLDRNVMRPLFSRDGGHVYFLVEDGGVQHVAR
                     IPAAGGTAERVVAGEREVLGYDLAPNGEMVVLESTPAYPAEISRARDGALTRISKVND
                     EFLKGIALGDTRRFKAKSPDGTMVDGFLTLPPGYKEGTRLPAILRIHGGPAAQFAYTF
                     DFELHILAAQGYAVITSNPRGSTGYGTAFSRAIWADWGNKDSEDVLAAVDHAVAMGVA
                     DPERLGVGGWSYGGILTDHVITKTTRFKAATSGASAANILAGYGTDHYQYEYEVELGL
                     PWKARDAYLRISGSFFDVEKIETPVLYLCGQDDMNVPLLNTEQLYQAVRRINKVPTEL
                     VIYPGENHVPRRPGSIKDVYERYIAWYDRFLKPAGAAAAGATPEATSLLGRPLYAPAL
                     AADAKAASDARLAAARDEFVKAPDSADTILWLGRQLAVAGRVREAIEVFTRGVEKFPN
                     DARFYRHRGHRYITARQLDKAVADLAKAGQLIAGKPDQPEPTTADPKVMSSETLHFAT
                     WYHLGLAHYLRGDFEKALDAYRKCLASAKGNDDQTVAITDWLYMTLRRLGRADEAARV
                     LEAVTPGMKVKDDQTYYDRLFMYKGLHAPEDLLRAGGDPTTAATLAYGVANWYLYNGR
                     KDEAKALFEQIVNSGGNWMPFGFIAAEAELARMKAQ"
BASE COUNT         1743 a         4542 c         4355 g         1990 t
ORIGIN      
        1 tcctcaaagc ggctccggcg gcggactccg ctcccgctga cgccgcggcc cgccacccgg
       61 cgccaggtct gccccgcgcc ggtttccttc agccacagct cggccttctc cgccatgaat
      121 tggccgatct cgacgtcgtg cgggtggcgg ctcccggaga tgcgtgccac cgggatgccc
      181 cagtgatcct tcacgacggg atcgacctcg acccgcgact cgaacaccgg catctcctgg
      241 atgggtccct gcacgcccac gcttcgccgg tagtacttcc gctggaactc cttgtgcccg
      301 cgcccccacc tcgccgaccc tggcgggcgc acgttggtga agaggtacgg caggcggatg
      361 aactcgttgg ccagcatcgc gccgccgcga atcccggggt tcccgtggtt gaagtcgcag
      421 atgccgatcg ttgcgcccgc gccgacatcg tcgtaggtct cctgctcgaa gaggccgtac
      481 gcgccgctgt aggcgtgccc ctgcaggttg cgcccgaccc agtcgtaccg gttgcccagg
      541 ccggccggga acaggcgtga cttcgagttc aggagcaggc gggccgactc ggtggccgac
      601 gccgatatga cgacgaggtc ggcctcctgc cgctggagac ggtcgcgcgg gtcgaagtac
      661 gcgacgccgg ttgcccgccc gcgggcgtcg gtcagcactt ccttcgccac gcagccggtg
      721 aagacgcggc agttgccggt gcggatggcc gccgggatga cggtgttctg cgtgccgcac
      781 ttcgcctgca cctcgcaggc aaacccgacg cacgaccggc agtgaatgca ggccgcgcgg
      841 ccgtcgcgcg gcaccgagtt gcgcagcatc ggcagcggga aggggtggaa gccaagccgc
      901 ctggccgagg cctcgagcag gcgcccttcg gtgttgtagg cgaacggcgg catcgggtag
      961 ggcttgcggc gcggcgcctg gaacgggttc gcgtccgcgt cgcccgccac gccgatctcc
     1021 tgctcggcct tctcgtagaa gggctcgagg tcgtggtagg tgatgggcca gtcctcgagc
     1081 gtgctcccct ccgggcaccc gtagatcgag cgcatccgga agtcctgttc catgaagcgc
     1141 caggccatcg cgccgtagct gagcgtgccg ccgcccacgc accccgcgtt gttgccgtag
     1201 gcgccctcgc tcggcagcac ggtctggaac cggtcgccga gccggacgac ccggcggtgc
     1261 cgctcgtcgt cgggcccgaa cgcgttcccg agaaccgtgg tgcgctggga gatcagctcg
     1321 tcgtggtcgt ggtcgtcgaa ggtctgccag cgcccgcgct cgagcagcac caccgagagc
     1381 ccggcggtcg ccagctcctt ggccacgacg ccgcccgccg cccccgcccc gacgaccacc
     1441 gcgttcactc gtccttgtgg cataggttca cgccctgcat gtccactgtc cacgttcacc
     1501 acagaggcac agaggcacag agattacccc ttcaccacca cgcgcccgag gacctgcggc
     1561 gtgtcgaggc ccagcatctt ccagctggcg aagttgcggt tgccgccgtg ccgcgggctt
     1621 ccgtagtacc cctgcaggca gtggtcggcg accagcctga agaactcgcg cgcctcgccg
     1681 ggcgcccaga tgccctcggg cacccggtcc gcctccagcg cctggaggag cgccgtctgg
     1741 cggtcgaacg ccagctcggc gaacggtgcg cccgccgtgc gccggctcgt ctcgtcgatc
     1801 ttcacgagcc ccgatcggta agcctggcgg tgccgccggt acgggccgcg cagctggcgg
     1861 tcgatgaact cggccacccc tgcgtccttc ccgcccggca cgtcgtcggg ggggatcacc
     1921 tggtcggcca gcgcctcgac gagccggcgc tcttcagggg tgaaggccgg cgccggctgg
     1981 aagcgcgcgc acgccgacca gaaggcgctg gccgccgcgg agagcgccga gacgagaatg
     2041 aactgccggc gggatagcgg atcctgcatg tcgaacggtc ctcccaacgc gcggctgcca
     2101 gcccgtgttt ctacatccga ttccgagggg tcgggagtaa ttagaagtgg tgccgacttc
     2161 taattactcc cgacccctac gacgcggccc aggggtgtgc taccagtgcg cctcgtacca
     2221 cgcgatcgag cgctcgatgc tgtcgatcac gtgcactgtc tcgcgcagcc cgtggccctc
     2281 gcgcgggtag cgtaccatca ccgtctcgac cccggcgtcc ttcagcgcga tgtagaactg
     2341 ctcggcctcg gcaatcggga cgtcgctgtc gttctcgccg tgcagcaaca gcaccggcgt
     2401 cttcacgttc gccacgtgcc gcaggggcga ccgctcccac aggacgtcca tcagcaggcg
     2461 cggcttcgcc ccggcgtcgg actgccactg ctcgtggggg tagccgccga actccaccgc
     2521 caggtagtcg tggtagtacg cgaggtagtt ctgcgtcacc aggttcgaga tgccggccgt
     2581 cggaatcgcc gccttgaagc ggggcgtgcg cgtgatcagc cagttcgtca gctgcccccc
     2641 gtagctgacc ccctcgatgc ccagccgctg cgggtcgagc caggagtact tcgccagcgc
     2701 cgcgtccacg cccgccagca cgtcgttcgc ctcgccgccg ttctggtcct tgaagatggc
     2761 atcggcaaac gcctgcccgt acccggtgga tccgcggtag ttcaccatca gcaccgcgaa
     2821 gccgtgagcg gcgtagatct gcgccttcgc gttgaagacc ggcccctgct gggcgtgcgg
     2881 cccccccttg atcatcgcga tcagcgggtg ccgccccccg gggtccacgc gcacgggccg
     2941 cgtcaggaac gcctcgatcg gggtgaggtc gccgctgccg tattgaatgc tctccaccag
     3001 cgcgagggtg cggcctgcca gcagcgggcg gttgagcgac gtcagctggc gcggcgtcca
     3061 gtcctgctgc cgccggaaca gctcggccgg gcccgaccgg gtcgccatcg agaaggccac
     3121 cgttctcccc cgccccactg agaacgacga cacggcgcca gcgtcggtgg ggtcgggggc
     3181 aaccggcgac ggcgcgccgc cggacaccgg gagccggtag agccgcacgc ttccccgctc
     3241 ctccacggtg aagtacaagg cgtcgccttc gggcgaccac gcggggcgct cctgcctgtt
     3301 gtcgatcgcg gcgcccgcct cgcggcggtt ccgcccctcc gcgtccatca cccacacgtg
     3361 ggtgtcctcc atcgtcgtct ccgaggaggt gagcgggcgc gtggtgccga ggaaggcgat
     3421 cgtgcggccg tcgggcgacc aggcgggctg gtactccgcg ctccgcgtgt cggtgaggcg
     3481 cctcaccgtg cggtcggcga catcgaccgc gaagaggtcg ttgttgaaga cgcggtcggg
     3541 gtcgggctcg cggttcgaga cgaagaggat ctcgtcgccg cgaggggacc aggcgatcga
     3601 gtgctcgtaa tacgggccgt cggtgagctg gcggacccgc tgccccgggt gcgcggccag
     3661 gtcggcgacg aagacgtgca gccggcggtt gtcgttgaaa cgggtcatcc cctccgaggc
     3721 cgtgggcttg tagaggtagc gcgtgatgac catggggtcg ccgctcgcct cgtcggtctc
     3781 gggccccggc gtggaagaga cgaacgcgat gcgcgtcccg tcgggcgacc acgagatgcg
     3841 ctcgccttcc ccgggcagcg gatggttggt gccactggtc gcgacgagca ggcgtgcgtc
     3901 cgagccgtcg ggttgcgaga ccatgagtcc ggcgccctcg ccggcatcgt ctccgcggtc
     3961 gagccaggcc accagcgagc cgtcgggcga ccagcggccg ttggacgact cgtttccgag
     4021 cggcgtcgaa cggccggtat cgagatccgt gatccaggcg cgcgaccgcg gccgcccggg
     4081 gctgtcggcg ctggtgacgc tgtaggcgat gcggcggccg tcgggcgaga gcctgacgtc
     4141 gcccaccgac cgcagccggt agaggtcgct cgtggcaagg tcgcgcatcg ctgcctgcgc
     4201 gcggacgggg accgggtcgc cggcggcggg cccgcgtgcg gaagggctcg ccgagggaag
     4261 ctgtgcgagg tgcagcaagg cgaggcctga cccgaggatc agcactgcgc gcatcgggga
     4321 ctccttggcg gtaagcgaac gggggtagca taccgattcc cgcgccgatt ccaatcgcga
     4381 ttcgtcgatc tgtcacggtg gggccgtacc gcgagcgtga cgtttttcac ctttttggtg
     4441 cggccccggg aagtgatgtt tttcacgctt tcggtacggc cccaggggtg tgattggatt
     4501 aggatccccg catgaacatc cgccgcttcc cgacgtccac cgagctggcc gacgccgccg
     4561 ccgcccaggc gaccgaggcc ctcacccgcg ccatcgccgc ccgcggccgc gcgcgcatcg
     4621 tcgcggccac cggctcggcg cagttcgagt tcctgtccgt cctcacgcgc acgcccggca
     4681 tcgactgggg gcgcgtcgaa atgttccacc tcgacgagta cgtcggcctc tccgccgatc
     4741 accccgccag cttccggcgc ttcctgctcg agcggctgat ccggccggcc ggcatcggca
     4801 cctaccacct gctcgacggc gacaccggcg accccgaggc ggtgtgcgag cgcgtcgggc
     4861 gcgagctgca ggcggcgcct gtggacgtca ccttcatggg catcggcgag aacggccaca
     4921 tcgccttcaa cgacccgccc gccgacttcg aaaccgagcg gccgtacatc gtcgtcgagc
     4981 tggacgaggt gtgccgccgc cagcaggtgg gcgaggggtg gttcgcgtcg ctcgccgagg
     5041 tgcccacgcg cgcgctgtcg atgtcggtga agcagctcct ccgctcggac gagctcatct
     5101 gcatcgtccc gcagtcgcgc aaggccaagg cggtgcaggc ggcactcgag ggcccgatca
     5161 cgccaatggt ccccgcgtcg atcctgcgca cgcacccgaa cgtgacgctc tacctcgacg
     5221 cggagtcgtc ttcgctgctc cgtgaagccc ggtcgtaaat tcgtcgctca cgcgagctgg
     5281 ggcggacagt cggccaaacg gtgactgtcc gcacccacgc acttgtgcag cccggttccc
     5341 agcccgggtc cccgggtcgc cacaacccgc tccgcctccc acaccgtggg ggcgttcgaa
     5401 cgatttcgtc ccagaaaacg ttgcacggac tctccacggg ttctcccaac gacctggcac
     5461 acgactggct tccagcttcc acagcgtgcc cggccgggaa gcagccgcgc ccaggattcc
     5521 tggcgcccgg cgcttccagc ccccaggccg ggcgcgtgaa ctcatccatg gccttccagg
     5581 atcattactc cctcctcgcg gcgcggtacg cccgggcccg ccccggttac ccggacgcgc
     5641 tcttcgacta cctggcttcg gtcgcccccg gccatggcct ggcctgggac tgcggaacgg
     5701 gcaacggcca ggcggcgcgc gggctcgccc gccgcttcgc ctgcgtccac gccaccgacg
     5761 caagcgcaga gcagctgcgt cacgccgagc cccacccgcg cgtttcgtat caccgcgagc
     5821 ccgcggagcg cgtctcgctc agggacgaga gcgtggacct ggtgacctcg gcggtcgccg
     5881 cgcactggtt cgacctcgac cgtttctacc ccgaggtcag gcgggttctg aagcccggcg
     5941 gcgccgtcgc cctctggacc taccatctgc cgcgcgtgtc tccggctgtg gatcggctgg
     6001 tggcctacta ctactccacg ctgctcgccg gccattggcc cgatcggatc agctacgtgg
     6061 accagcggta cgccacgctc ccctttccgt tcgacgagct cgaaccgccg gccctcacca
     6121 tgacgacctc gtggaccgcg gcccagctgg ccgccttcct cgcgagctgg tcggcggtgc
     6181 ctcgctatca gaaggcccac ggccgccacc cggtggacga gatctgggac gacctcgtga
     6241 ccgcgtggga aggcgacgac gccacccgca ccatcacctg ggacttgcac ttgagggtgg
     6301 gcttaagcag gtgacttcgt cgactgtggc ttggcgctag ccaagccacc gggggttctg
     6361 tgccgtggag tcgtgctccg cggtcgtctt gtacggctcg aaggcgcggc cgagggcggg
     6421 ctggcgcgtg cgctcccgca gggcgttcat ctcgtcggcg gtataggggc tgaaggctcg
     6481 cgcgacgcgc acggcctggt ccagcacctt catggagtcg caacctgtga tgacggtgct
     6541 ggtcggcagc gagagggcgt agcgcaggca ctcctcggcc gacacggtat cgctctcgag
     6601 cagcttcccg gcgccgagcg gcttcatgcc gagcacgccg atgccgcgtg cctccagcac
     6661 cggcaacacc atcttttcga agctctcgac gtgcgcgtcc atgggattga gcggcagctg
     6721 caccgcgtcg aacgtgaagc cgtgggcgtc ggcgaccgac agcatgtgga gaaggatctc
     6781 ggggctcttg tggccggtga agccgatgaa gcgcagcttc ccggcctcgc gcgccgccac
     6841 caccgcctcg agggccccgc cgggcgcgaa gatccggtcg gcgtcctcca tgcggatgat
     6901 ctcgtggaac tgcagcaggt cgacgcggtc ggtgcgcagc cgcagcagcg attcctcgag
     6961 ctgggcgttc gccgactccc acgtcctccc gtcgatcttg gtcatcagga acgcgcgctc
     7021 acggtagccg ccggccagcg cccggcccat ccgttcctcg ctcgcgccgc cgtggtagtc
     7081 ccacgagttg tcgaggaagg tcatgccgtg gtcgacggcg gtccggatga ttcgaacggc
     7141 ctctgcctcc tcgatgccgc cgaggtggaa gccgcccacg cccacgatcg acacccgcgc
     7201 acccgtccgt cccaggctcc gttgaggcag ctgctgctcc atgccacagg tgctgcaacg
     7261 gtagcgccac ctgcggtccc gggcggcagc gccggtgcgt ccgcggctag aaccgacagc
     7321 gacccgcact gaatgcgtac ggcccgcaac cgtgatcggg atccgggtcc tgtctggctg
     7381 cctcgccacc cgctccggtt gcgggcggtc aacgccggcg cccgcgaaag gcgggacgtg
     7441 gccggctcgg cggctgatgc gacgggtgca tcggctcccg agccgcactc cgcagtccgg
     7501 gaacgcgtcc gtgtcgcgat cgaagggctg tcggagatcg cggaagctga tgagcgagct
     7561 gaaggcgttc ttgtcaccgc tcgtacgact cgcggcgcgc ggcgcgcgac agccggatca
     7621 cgtagcgcga ggggtcggcc aggaagcgct cgcgcagctc cggcaggctg gcggccccgc
     7681 cgtagctgat ggccgagcag aggtgcttga tcatgtcgcg gacgatgggg cggaccgagc
     7741 cgcgctcggg gaccgccacc tcgaacccct cggcgccgat cgcctcgagg tcggccgcgg
     7801 ccgcgtcctc gacgtcgagg cggtccttga tcgcctcgat cgacgccatc ccgcgcagca
     7861 ccttgaaggg gatcttcacc gacttctgcg actcgggcac caccaccgcc ttgcgcacca
     7921 cctcgcccgg cgcctcgcgg gtgccggcga acgcgctgcc cagcatcacc gtgtcgccgc
     7981 cgaacagcag cgcctgcacc agggcgccgt cgcggcgcac gccgccgtcg gcgatgatcg
     8041 ggaggcgcgt cgccatccgg cactccacca gcgcctgcac ctgcggcacg ccgaagctgg
     8101 tggtcagcct cgtcgtgcag cctccgcccg ggccgatgcc taccttgatc ccgttggccc
     8161 cgcgctcggc caggaaccgc gcgccttccg cggtggccac gttgcccgcc accagctcga
     8221 cgtcggggaa gcgcttgcgg aactcctcga gcgcgcgcgc catcacgacc gagtgcccgt
     8281 gcgcgatgtc gatgacgatc aggtcggtgc cggccgcgac gaggccggcg gcccgctcga
     8341 ggtagtcgcc ggtggcgccg atcgccgcgc ccacccgcag cctcccctgc gcgtcgcgcg
     8401 tcgcgaacgg cagccgcttc tgcttcagga tgtccttcgc ggtcaccagg ccgaggagcc
     8461 gccgcccagc gtccacgagc ggcagcttct tcaccttccg ctcgaccatg atccgctccg
     8521 cctcgtcgag cgcgatggcc ccctcgtgca cgaccagccg gtcgaacggc gtcatccgct
     8581 cggcaacctt gacgtcgccc tcgacgaagc gggtgtcgcg ctcggtcagc agcccctcca
     8641 ggcggttctg cgcgtccacg acgaccagcg tgccgacacc cgagcggcgc atcgcgtcgg
     8701 ccgcctcggc cagcgtggcg tgcggcgcga tggtataggg gtcggggatc acgccgtgtt
     8761 gcgtgcgctt gacgatctcc acctcgcgca cctgcggcca gatctcgccg gggcggaagc
     8821 cgcggtcgat gatgccgatg ccgccttcct cggccagcgc gatcgccatc gccgcccgcg
     8881 tcaccgtgtc catgttggcc gagacgatgg ggcgccgcag cacgaggtgc tccgacagcc
     8941 gggtggagag atcgatgacg gccgggtcgc gccgctcgag cacgctgaac tgcggcgcga
     9001 acaggaagtc gtcgaaggtg cagccgcgga ccagagacgg cagatcccga gacacgtcac
     9061 gactcgaatg ctgcattcca gcattgtccg acacattcgc cgtccggatg agtgtgaacc
     9121 ttacgcaccc tcaacgcatg ccagctggac agagggattc ggcggcactt cgggatagtt
     9181 tgagaacagg cgttgaggcg gcgggctact ccaccgagat gaccctggcg ccccaggggc
     9241 agaaggtacc gtcggcaaag gatggcccgc cggccgcctc caccatcgat ggccgcccgt
     9301 cgcaaacctc gatggtcacc tccacgaagc gcacgtcctc gaggtgccag ctgtagccgg
     9361 cgttgacctg cgtccccgcg acgatgcggc cgtgggggat gctggcgcgc ccgccggcct
     9421 gcgctgcctt cgccgccgca acctgctcgt cggtcgtcag cagcacccgg aaggtttcgc
     9481 cgctgaccag gatcctggcg acgacgccgc cgcccggcgc ggtgggtgaa tcggccccgc
     9541 agccggcgag ggccaccgcc gcgatgaagg ccaggagcgg gaggatggcg gaagttgtga
     9601 gcgaggtggg gcgtcgaacg ctggacatct gtcccctccg ggcgacgcgc gccgcgccgc
     9661 caggaggggg actgcaaatc ctcggccgag ccagatgagg gccggacgcg tttacttcta
     9721 gcgcgcgcac ccgccctcgc cgcttcgcgg ctacggcgcg ccaggcgggg gctggcgcta
     9781 ctgggccttc atgcgcgcga gttcggcttc cgccgcgatg aagccgaacg gcatccagtt
     9841 gccgcccgag ttgacgatct gctcgaacag cgccttcgcc tcgtccttcc ggccgttgta
     9901 gaggtaccag ttcgcgacgc cgtaggcgag cgtcgccgcg gtggtgggat cgccgcccgc
     9961 gcgcagcagg tcctcgggcg cgtgcaatcc cttgtacatg aagagccggt cgtagtaggt
    10021 ctggtcgtcc ttcaccttca tgccgggcgt gacggcctcg agcaccctgg cggcctcgtc
    10081 tgcccgcccg agccgccgca gcgtcatgta gagccagtcg gtgatggcca ccgtctggtc
    10141 gtcgttcccc ttcgccgacg ccaggcactt gcggtaggcg tcgagcgcct tctcgaagtc
    10201 gccccgcagg tagtgcgcga ggccgaggtg gtaccaggtg gcgaagtgca gcgtctcgct
    10261 cgacatcacc ttggggtcgg cggtggtcgg ctcgggttga tcgggcttgc cggcgatcag
    10321 ctgccccgcc ttcgccaggt cggcgaccgc cttgtcgagc tgccgcgccg tgatgtaccg
    10381 gtgcccccgg tggcggtaga agcgggcatc gttcgggaac ttctccacgc cgcgcgtgaa
    10441 gacctcgatg gcctcgcgca cgcggccggc caccgcgagc tgccgcccga gccagaggat
    10501 ggtgtcggcg ctgtcgggcg ccttcacgaa ctcgtcgcgc gccgcggcca gccgcgcgtc
    10561 ggaggcggcc ttcgcatcgg ctgccagcgc gggcgcgtac agcggccgcc cgagcagcga
    10621 ggtggcctcg ggcgttgccc ccgcagccgc cgccccggcc ggcttcagga agcggtcgta
    10681 ccaggcgatg tagcgctcgt agacgtcctt gatcgacccg ggccggcgcg gcacgtggtt
    10741 ctcgcccggg tagatgacca gctcggtcgg caccttgttg atgcgccgca ccgcctggta
    10801 cagctgctcg gtgttgagca gcggcacgtt catgtcgtcc tgcccgcaca ggtacagcac
    10861 cggcgtctcg atcttctcga cgtcgaagaa cgaccccgag atcctcaggt aggcgtcgcg
    10921 cgccttccac ggcagcccca gctcgacctc gtactcatac tggtagtggt cggtgccgta
    10981 gccggcgagg atgttcgccg cgctcgcccc cgaggtggcc gccttgaacc tggtcgtctt
    11041 cgtgatgacg tgatcggtga ggatgccgcc gtagctccac cctcccacgc cgagccgctc
    11101 gggatcggcc acgcccatcg cgacggcgtg atcgaccgcg gccaggacgt cctcgctgtc
    11161 cttgttcccc cagtcggccc agatcgcgcg gctgaacgcg gtgccgtagc cggttgaccc
    11221 gcgcgggttc gaggtgatca ccgcgtagcc ctgcgcggcg agaatgtgca gctcgaagtc
    11281 gaacgtgtac gcgaactgcg cggccggccc gccgtgaatt cgcaggatgg ccggcagcct
    11341 cgttccctcc ttgtatcccg ggggcagcgt gaggaacccg tccaccatcg tgccgtcggg
    11401 cgacttcgcc ttgaagcgcc gcgtgtcgcc gagcgcaatc cccttcagga actcgtcgtt
    11461 caccttgctg atgcgcgtca gcgcgccgtc tctcgcccgc gagatctcgg ccgggtacgc
    11521 gggcgtgctc tcgaggacga ccatctcgcc attgggcgcg aggtcgtatc ccagcacctc
    11581 gcgctcgccc gctacgaccc gctcggccgt gccgccggcg gcgggaatgc gcgccacgtg
    11641 ctggacgccg ccgtcctcga cgaggaagta gacgtggccg ccgtcgcgcg agaagagggg
    11701 ccgcatcacg ttgcggtcga gcggcgcggt cagcgccgtc gccttcgccg tcgcctgggc
    11761 cgtcgcctgc gccgccccgc tgccgttcca ctcgccgggc acttcgacga tgccgatgtg
    11821 ggtggcgccg taccacatgt cgttcggatc gccgctggac acgaaggcga gccgcctgcc
    11881 gtcggggctg aacaccgggt tcgactcggc acgatcggtg ttgacgagcg ggcgggggat
    11941 gccgcccttg gcgggcacca cgtagatgtc gctgttctgg ttccggtcgg ggtcgggcag
    12001 cgtccggttg ctcgagaagg cgatgagggt cccgtcaggc gaccacgccg gatcgccatc
    12061 gtcgaacggg cccgaggtga gctggcggct cgacttcgac gccacgtcga acacgtggat
    12121 gtggctgcgc aggtcggtca ggtagccctg gtagtcgcgc ttgaactgca gccgcttgac
    12181 cacgatcggc ttcttcgcct tcttgtcgtc gcccttgccc tcttcctcct cgtctgcggg
    12241 agcgtcggga tcgacgtcgt ccacgaccag cgcgaggcgg gtgctgtcgg gcgaccaggc
    12301 gaggtcggag acgccggcct tgtagctggt cagcttgaag gcctcgccgc cggagagcgg
    12361 cagcagccac acctgggcgt gcgagccggc gcgtcccgag aggaaggcca gccacttgcc
    12421 gtcggggctg aagcgcggcg cgcgctcggg cttgtcgctg gtggtgaggc ggacggcctc
    12481 gccgccggcc acgggcgcca tccagatgtc gctgtcgctg gtgtccttct tcaggtccat
    12541 cgaggtgacg gtccaggcga cccacttgcc gtcggggctg aggcgcgggt cgcgcacgtc
    12601 cttgagggcg aagtagtcgt ccactttgag
//
LOCUS       DAOPQX010000071        12610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5806_length_12610_cov_4.323457, whole genome shotgun sequence.
ACCESSION   DAOPQX010000071 DAOPQX010000000
VERSION     DAOPQX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12610
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5806_length_12610_cov_4.323457"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKK95_05735"
     CDS             complement(<1..1100)
                     /locus_tag="PKK95_05735"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOC17746.1"
                     /translation="MDHREGDTPVDSAAVLAALRAVHDPDLHKDIVSLGFVKDLRIEG
                     GRVAFTIELTTPACPVKDAMQEQARQAVAAVPGVSDVQVTMTAAVRTAHNPASQQAPV
                     PGVKNVIAVGAGKGGVGKTTVAVNLAVALARLGSRVGIVDGDIYGPNVPIMLGIDSPL
                     LAEGEKILPPERYGLKAVSMGFLANDDSPVIWRGPMLHSVIRQFFHDVKWGELDYLVI
                     DMPPGTGDVALSLSQTVPVAGAIVVTTPQAVSLADSRRAVRMYQKLNIPVLGLVENMS
                     YFACPNCGHESDIFGRGGGERVAGDMQVPFLGRVPIYEPIRSGGDTGSPLVILEPESP
                     AARAFTAVAEQTAAQVSIASYRKPVIPLAPAR"
     gene            complement(1388..3415)
                     /locus_tag="PKK95_05740"
     CDS             complement(1388..3415)
                     /locus_tag="PKK95_05740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC17747.1"
                     /translation="MRHRPIAVTLAVLALVVLAAGAAAQPAGRPLSLDDLARLRVVRE
                     PDLSPDGRWIAYVVATVDAEKDKRDTDLWMVAWDGSEKVRLTATADSAERQPLFSPDG
                     RYLAFLAARGDEDEKKKGAQVWLLNRAGGEAQKLTDAKGGVSDFAWSPDGKRLVLVVG
                     TFDPASDPEKMEGWKRKTEPPIVIDRYQFKTDEGGYLKRFPSHLALLDLESKREEVLT
                     SGPYDESEPAWSPDGRSIAFISNRSEPDPDRTVDTNIYVIEARPGAEPRQVTTYPGHD
                     MGRPAWSPDGRWIAYYQGEEAKYAAYELNKLAVVPASGGAGRLLTPSLDRSGQGDIVW
                     AADGSSLCFVVEDDRASYLARVPASGGPVERLTTGRRTVAWMAPGPAGAFALLTATAS
                     EPGDLHALEKGQLRRLTRENAWLSEVRLAVVDDFTAKSKDGTIVNGLVAKPSGFEAGK
                     KYPTILYIHGGPVGQDDYDFDFNREIIAASGYLVLQANYRGSSGRGAEYQKAIFADWG
                     NKEVVDLLAAVDWAVAEGIADPDRLGLGGWSYGGILTNSTIATDSRFKAAVSGASSSL
                     QLSMYGSDQYIVQYENELGLPWKSRDAWLKVSYPFFHMDRITTPTLFLCGLLDFNVPI
                     VGVEQMYQGLKSLGVDTQLVVYPGQHHGIVTPSYARDRLERWLAWWNKYLK"
     gene            3535..4002
                     /gene="bfr"
                     /locus_tag="PKK95_05745"
     CDS             3535..4002
                     /gene="bfr"
                     /locus_tag="PKK95_05745"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOC17748.1"
                     /translation="MKGHADIIKTLNDLLAEELTAINQYMVHSEMCDNWGFEKLHKAI
                     EKQAIDEMKHAESLIARILFLEGSPTVSRLNQINIGKTVGEMLGNDLDAEAHAVKMYN
                     DAVQQAQSLRDEGTRDLLRQILHDEEEHLDWDEQQRDLIEQMGIENYLASQTS"
     gene            complement(4113..4730)
                     /locus_tag="PKK95_05750"
     CDS             complement(4113..4730)
                     /locus_tag="PKK95_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17749.1"
                     /translation="MRIAAAALAVATLAGPASHAAVPAAGDERAGRAQISDAVALGMS
                     RDEARRAAFHERYVSRLGGPLVRSLEVVTEFRRAVLLAEGHARLGEPAWLTERAAAEL
                     APFRGRLDLVLRLRFDPLNSYRAMPEVGVVIYRQEEGALQPSSLRATPENFAGPVPPG
                     TPILAATVEAAFPLSALDTSRPFLLGIFLDGREVQRLPVDLAGLR"
     gene            complement(4727..4990)
                     /locus_tag="PKK95_05755"
     CDS             complement(4727..4990)
                     /locus_tag="PKK95_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17750.1"
                     /translation="MGDGAEGLVDAEARIQDRLGELEQQRATRRRRVKDPELVRELES
                     LRLARAEAHRQAEATAHPVRREQLQQAMAELDRRITDVMGKLE"
     gene            complement(5137..5943)
                     /locus_tag="PKK95_05760"
     CDS             complement(5137..5943)
                     /locus_tag="PKK95_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17751.1"
                     /translation="MPEVRATAGEREAAQRVYREGLDVLDALGLPFLVGGAYAFARYT
                     GIERFTKDFDVFVDSASLEPVLGALAAAGFSIDRPFPHWLAKAYRGGYYIDVIYSSGN
                     GIARVDDRWFEHAVAGEVLERPVRLVPPEEMLWSKSFIMERERYDGADIAHLLRALAS
                     TLDWRRLIEHFGGYWRVLLSHAVLFGFVYPGERDRIPAWVMETLVGRLEQDLRTPSSD
                     ERICQGTILSRQQYLPDVELWGYHDARVFPRGHMSADDTATWTAAIGDDK"
     gene            complement(5993..6790)
                     /locus_tag="PKK95_05765"
     CDS             complement(5993..6790)
                     /locus_tag="PKK95_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC17752.1"
                     /translation="MDPRKTEVRLAALADIHFTRSSQGSLQGVLTQAAAAADILLLAG
                     DLTSYGLPDEAQLLVRELTAAAKIPVIAVLGNHEYESGKEGEVASILAESGVRVLDGE
                     PYELMGVGFAGVKGFGGGFDRRALEPWGEATMKQFVHDAVQETLKLGSALGRIRAERR
                     VVLLHYSPIRATVEGEPLEIFPFMGSSRLEEPIDRYRVNVVFHGHAHHGRPEGRTRGG
                     VQVFNVGMSLLQRHYPDRPPFRLFTLPLVRGPARQEGASEAFGSHVP"
     gene            6955..7740
                     /locus_tag="PKK95_05770"
     CDS             6955..7740
                     /locus_tag="PKK95_05770"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HOC17753.1"
                     /translation="MEDVRVSSWNELHEVLYEGSWQERLGRFRSRYAFRGQSEVGEEL
                     TSSLARLGAPPEVEGHLLRNFRKYAQREAAPGDSIWNWLAVAQHHGLPTRLLDWTFSP
                     YVALHFATEHTEKYHLDGVVWQVDYVATNQLLPERLKEILRDEGSDVFTAEMLARASA
                     TLAGFDRLAAGEFVAFFEPPSLDERIVIQFALFSLMSGPHANLKAWLDEHPRMFRRVV
                     LPASLKWEVRDKLDQANITERVLYPGLDGLSRWLRRYYMPRRR"
     gene            7841..8704
                     /locus_tag="PKK95_05775"
     CDS             7841..8704
                     /locus_tag="PKK95_05775"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOC17754.1"
                     /translation="MIGEVLLESVYDTLRMLPVLVPVYILLEYLSHREVPGLVHRFRL
                     GGPAGPLVGTLLGIIPQCSMSVLVTSLFASGRVSLGTVLATYLATSDEALPLMIAEGR
                     HARVVVAFVALKVAIALAAGYLADLVLGRRYADLPPTGRVIHSHTEKASWAEIVRHGV
                     RHSAVVAAWVFSATFLLGLILAWGTGRVSWLAGSPGWSVPRVAGVTLFGMIPNCAASV
                     AITEAFLKAGLPFGTAVAGLSAGAGFGPIVLLREVRLRQALILLGWLAAIAMAAGLVI
                     DAWYPFSIPVR"
     gene            8707..9162
                     /locus_tag="PKK95_05780"
     CDS             8707..9162
                     /locus_tag="PKK95_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOC17755.1"
                     /translation="MAPLREPSSRDTSQRRAIRYAVESAGRPLSAAEILASARREVPG
                     LGIATVYRAVRRMQEEGTLRQVDLPGDAPRYESSGKGHHHHFHCRSCSRVYEVEACPG
                     EFVMQAPPGFSVEDHELILYGRCPTCVAPRRTGAPRSRGSRTVSRGRER"
     gene            9159..9875
                     /locus_tag="PKK95_05785"
     CDS             9159..9875
                     /locus_tag="PKK95_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17756.1"
                     /translation="MMAEILVVIWLLSQARDPALPLPGVTSAGPHLSQEPEAQEQGDG
                     QARFNPAISVIPDVAYYNDNRDGAAGALVSGADGFARHGEGGGNAHGSDLERGFNLRE
                     VEVAFSGAVDPYFDASAILAVGDGEVEAEEVYIQTRALAPGLQLRAGKFYSGIGYLNA
                     KHSHQWDFVDRPLPYSTCSAARLARSGCRQPGFPHSPSTCSSAANCSRAPTRRSRSSW
                     QRRRNRASPRRAARGSSRDS"
     gene            9872..10438
                     /locus_tag="PKK95_05790"
     CDS             9872..10438
                     /locus_tag="PKK95_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17757.1"
                     /translation="MKVSPGLGDSHTLQVGSSIAHTRSHQEFAVEHGDEEPLEGSGTL
                     VGADLVYQYDSGKQWGVGDLTLQGEYFWRRHDLAVASRAGRLQRDRTQDGFYVQGVYG
                     FAARWKGGGRFDAAGLTNQLHVRDGLLRLESVRRYTAVLTFMPTEFSRLRVQYTRADS
                     PIRAVREKSNQLWVQLQISLGAHGAHTF"
     gene            10472..11365
                     /locus_tag="PKK95_05795"
     CDS             10472..11365
                     /locus_tag="PKK95_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOC17758.1"
                     /translation="MRIRLLLPLVAALALAVPAPAQTIDVVATTSSMGVLARVVGGPA
                     VKVTVLAPPDRDAHYLIARPSMMLALRRADLLVAVGADLEVGWLPAALRSAGNRKVLP
                     GQPGYFEGAAHVDLLEKGQAADRSRGDVHPAGNPHYYMDPLRLSGAARALASHLARLR
                     PGSATAFAANADAFAKAASERVVQWKARAAGAPGVLLFHKDVNYLADLLGVPIVGYVE
                     PVPGVPPTASHLRDLVTRLQGTRGVILHAAFQPSDGPQYLAKNLGWPVQRLQLEPPPD
                     ADLPAYLAHVERWVAAVASAK"
     gene            11362..11937
                     /locus_tag="PKK95_05800"
     CDS             11362..11937
                     /locus_tag="PKK95_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC17759.1"
                     /translation="MNGHLLLEVENLVAGYRDPVVGPVSFRLNRGEILGLAGPNGSGK
                     STVLRAIIGRARIFSGTVRRGHDPIATAVQAQRPVRLAEMPITGREYLRLTGAARRAP
                     PPQLGGLLDLRLDELSGGQFQLLHVWACLESPAGLVLLDEPDNNMDPGARATLIEVLH
                     QSRTAGRGVLVVSHEHDLVESVCSRIVEVAA"
     gene            11934..>12610
                     /locus_tag="PKK95_05805"
     CDS             11934..>12610
                     /locus_tag="PKK95_05805"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOC17760.1"
                     /translation="MSFADLLHPMFLIPFVNGLLLAILLPLVGAHVRLREEWLASLGL
                     AQAAAAGVMVGAFLDAPIAVVALTAAVVAALAKSFFGPKGNESYALMLLLGWTVALLL
                     AANSAHGDDLSHALTEGQLYFTGGSHLQALVAVGLVQFGLLPWLAPRLLLARFFPDHF
                     AANGRPNPHHEIVFDVLVAVTLALAATAIGVMAAFAVVFLAPWIAFRFARGWFKTIAL
                     GIAVSLAA"
BASE COUNT         1800 a         4491 c         4377 g         1942 t
ORIGIN      
        1 taccgcgccg gcgccagcgg gatgaccggc ttccggtagg aggcgatcga cacctgcgcg
       61 gcagtctgct cggcgaccgc cgtgaaggcc cgtgccgcgg gcgactcggg ctcgaggatc
      121 accagcgggc ttccggtgtc gcccccgctg cggatcggct cgtagatcgg gacgcgcccg
      181 aggaagggca cctgcatgtc gcccgcgact ctctcaccgc cgccgcgacc gaagatgtcg
      241 ctctcgtggc cgcagttcgg gcacgcgaag tagctcatgt tctcgacgag gccgagcacc
      301 gggatgttca gcttctggta catccgcacc gcccgccggc tgtcggcaag cgagacggcc
      361 tggggcgtcg tgacgacgat cgcgccggcc accgggacgg tctggctcag gctcagcgcg
      421 acgtcgccgg tgccgggcgg catgtcgatc accaggtaat cgagctcacc ccacttcacg
      481 tcgtggaaga actggcggat gacgctgtgc agcatcggcc cccgccagat caccggcgag
      541 tcgtcgttcg ccagaaatcc catcgacacg gccttcaacc cgtaacgctc gggcggaagg
      601 atcttctccc cttctgccag cagcgggctg tcgatgccga gcatgatcgg gacgttcggc
      661 ccgtagatgt cgccgtccac gatgcccacg cggctgccga gccgcgccag cgccaccgcc
      721 aggttcaccg ccacggtggt cttgccgacg ccccctttgc ccgcgccgac cgcgatgacg
      781 ttcttgacgc cgggaaccgg cgcctgctgc gacgccgggt tgtgcgccgt cctcaccgcg
      841 gcggtcatcg tcacctgcac gtcgctcacg ccgggaacgg cggccaccgc ctggcgggcc
      901 tgctcctgca tcgcgtcctt gacggggcag gcgggcgtgg tcagctcgat ggtgaacgcg
      961 acgcgccccc cctcgatgcg caggtccttg acgaacccca gcgacacgat gtccttgtgc
     1021 aggtcggggt cgtgaacggc gcggagggcg gcgagcacgg ccgcactgtc gacaggggtg
     1081 tctccttctc ggtggtccat ctcaccatct tgacacagag gaacagaaaa gggtgtcgtg
     1141 ctgggggtgc aggaggtgca gaaggtgccg agggtgctcg tgccggaggt gccggggtgc
     1201 tcgtgccgga agtgccgccg ggtgtcgggg gtgctcgcgc cggaagtgcc gggggtgcag
     1261 gtgcacttcg gcaccagcac ccgccttcgc cgcgcggtgc gcgggtacgg cgcggcgggc
     1321 cctccggcac ccttggcacc gcacctccag cacccgctgc acctcctgca ccccctgcac
     1381 gatctgtcta cttcagatac ttgttccacc acgccagcca gcgctccagg cgatcccggg
     1441 cgtagctcgg cgtgacgatg ccgtggtgct ggccgggata gacgaccagc tgggtgtcca
     1501 cccccaggct cttgaggccc tggtacatct gctcgacgcc gacgatcggc acgttgaagt
     1561 cgagcaggcc gcacaggaag agcgtgggcg tcgtgatccg gtccatgtgg aagaacgggt
     1621 acgacacctt cagccaggcg tcgcgcgatt tccagggaag cccgagctcg ttttcgtact
     1681 gcacgatgta ctggtccgag ccgtacatcg acagctgcag tgagctgctg gcgccgctca
     1741 ccgccgcctt gaagcgcgag tcggtggcga tcgtggagtt ggtgaggatg ccgccgtagc
     1801 tccagccccc gaggcccagg cggtcggggt cggcgatccc ctcggccacc gcccagtcca
     1861 cggcggccag caggtccacc acctccttgt tgccccagtc ggcaaagatc gccttctggt
     1921 actcggcccc tcgccccgag ctgccgcggt agttggcctg caggacgagg tagccgctgg
     1981 cggcgatgat ctcccggttg aagtcgaagt cgtaatcgtc ctgcccgacc gggccgccgt
     2041 ggatgtagag gatggtgggg tacttcttcc ccgcctcgaa accggacggc ttggccacca
     2101 gcccgttcac gatcgttccg tccttcgact tcgccgtgaa gtcgtccacc acggcgagcc
     2161 gcacctcgga cagccaggcg ttctcgcgcg tcagccggcg caactgtccc ttctcgagcg
     2221 cgtggagatc gcccggctcg ctcgcggtcg cggtcagcag cgcgaaggcg ccggcgggcc
     2281 cgggcgccat ccaggcgacc gtgcggcggc cggtcgtcag ccgctccacc ggtcccccgg
     2341 aggccggcac ccgcgccagg tagctcgccc ggtcgtcctc gacgacaaag cagagcgacg
     2401 agccgtcggc ggcccagaca atgtcaccct ggcccgagcg gtcgagcgac ggcgtcagca
     2461 gccggccggc gccgccggag gccggcacca ccgccagctt gttcagctcg taggccgcgt
     2521 acttagcctc ctcgccctgg tagtaggcaa tccaccgccc gtcggggctc caggccggcc
     2581 tccccatgtc gtggcccggg taggtggtca cctgccgcgg ctcggcgccg ggccgggcct
     2641 cgatgacgta gatgttcgtg tccacggtcc ggtcggggtc gggctcactg cggttgctga
     2701 tgaaggcgat gctcctgccg tcgggcgacc aggccggctc gctctcgtcg tagggcccgc
     2761 tggtcagcac ctcctctctc ttcgactcga gatccaacag ggcgaggtgc gacgggaagc
     2821 gcttcagata gccgccctcg tcggtcttga actggtagcg gtcgatcacg atcggcggct
     2881 cggtcttgcg cttccagcct tccatcttct cggggtccga ggcggggtcg aaagtgccca
     2941 ccaccaggac gagccgcttg ccgtcgggcg accaggcgaa atcggacacg ccgcccttcg
     3001 cgtcggtgag cttctgggct tcgccgccgg cgcggttcag cagccagacc tgcgccccct
     3061 tcttcttctc gtcctcgtcg ccccgcgcgg cgaggaatgc gaggtagcgt ccgtccgggc
     3121 tgaagagcgg ctggcgctcg gcgctgtcgg cggtggccgt gaggcgcacc ttctcggaac
     3181 cgtcccaggc gaccatccac aggtcggtgt cgcgcttgtc cttctcggcg tccaccgtgg
     3241 ccaccacgta ggcgatccac cggccgtccg gggagaggtc gggctcccgg accacccgga
     3301 ggcgggccag atcgtcgagc gagagcgggc ggccggccgg ctgggcggcc gcgccggcgg
     3361 ccagaacgac gagcgcgaga acggcgagcg tgacggcaat cgggcggtgg cgcatggcgg
     3421 tcgctcctgt cggaaggcag cccggcgcgc cggaccgacg tgcatgctac caccgtctga
     3481 agtattctct tgggctgccg gactgtgttg ccaacctgct acccgaggag cgccatgaaa
     3541 gggcacgccg acatcatcaa gaccctgaac gacctgctgg cggaggagct gaccgcgatc
     3601 aaccagtaca tggtccactc ggagatgtgc gacaactggg gcttcgagaa gctgcacaag
     3661 gcgatcgaga aacaggcgat cgacgagatg aagcacgccg agtcgctcat cgcccgcatc
     3721 ctcttcctcg agggctcgcc caccgtttcg cgcctcaacc agatcaacat cggcaagacc
     3781 gtgggcgaga tgctgggcaa cgacctcgac gccgaggcgc acgcggtgaa gatgtacaac
     3841 gacgccgtgc agcaggccca gtcgctgcga gacgagggga cgcgcgacct gctgaggcag
     3901 atcctgcacg acgaggagga acacctcgac tgggacgagc agcagcgcga cctgatcgag
     3961 cagatgggga tcgagaacta tttggcgtcg cagacgtcgt agcgcacagg cacacacagg
     4021 cgggacgcct gtgccactgc tacggctgcc gcggcgcccg cagccacaca ggcgggacgc
     4081 ctgtgccact gtgccagtgt gccactgtgc cgctaccgaa ggccggcgag atcgaccggg
     4141 aggcgctgca cctcgcggcc gtcgagaaag atcccgagga ggaaggggcg cgaggtgtcg
     4201 agcgccgaga gcgggaaggc ggcctcgacg gtggcggcga ggattggcgt tcccggcggg
     4261 accggccccg cgaagttctc cggcgttgcc cggaggctgg aaggctgcag cgcgccttcc
     4321 tcctgccggt agatcacgac gcccacttcc ggcatcgccc ggtaggagtt cagcgggtcg
     4381 aaccgcaggc gcagcaccag atcgaggcgg ccgcggaagg gcgccagctc cgccgccgcc
     4441 cgctcggtca gccacgccgg ctcacccagc cgcgcgtgtc cctcggccag cagcacggcc
     4501 cgccggaact cggtcaccac ctcgagcgac cgaaccaacg gtcccccgag cctgctcacg
     4561 taccgctcgt gaaacgcggc gcgcctggcc tcgtcgcgcg acatccccaa cgccactgcg
     4621 tcggaaatct gcgcgcgccc cgcccgttcg tccccggcgg ccggcaccgc ggcgtgcgag
     4681 gccggccctg cgagggtggc gacggccaag gccgccgcgg cgatcctcat tcgagcttcc
     4741 ccatcacatc ggtgatccgc ctgtccagct cggccatcgc ctgctgcagc tgttctcgcc
     4801 gcacggggtg cgcggtggcc tcggcctgcc ggtgggcctc ggccctcgcg aggcgcagcg
     4861 attccagctc gcgcaccagc tccgggtcct tcacccgtcg ccggcgcgtc gcccgctgct
     4921 gttcgagctc ccccagccga tcttgaatcc tggcctcggc gtcaaccagg ccttccgcgc
     4981 cgtcccccat agcctccccg tttcacctcg cgcgacgccc ctgccgccgc gcgccgcgcc
     5041 cgccccggcg ggttgcggcg ccacgcgtca ccgatccgca tcagtgatgg cgccctttca
     5101 agtatagccc gcgtcaccgt tttcacgccc ggggcgctac ttgtcgtcac cgatggcggc
     5161 cgtccaggtc gccgtgtcgt cggccgacat gtgcccccgg ggaaacacgc gggcgtcgtg
     5221 gtagccccac agctcgacgt ccggcaggta ctgctgccgg gaaaggatcg tgccctggca
     5281 gatccgctcg tcagaggagg gcgtccgcag gtcctgctcg agccgcccca ccagcgtctc
     5341 catcacccag gccgggatgc ggtcccgctc acccgggtag acgaagccga agagcaccgc
     5401 gtggctcagg agcacccgcc agtagccgcc aaagtgctcg atgaggcgcc tccagtcgag
     5461 ggtggaggcc agcgccctca acaggtgcgc gatgtccgcg ccgtcgtagc gctcgcgctc
     5521 catgatgaag ctcttcgacc acagcatctc ctcgggcggc acgaggcgga ccgggcgctc
     5581 gaggacctcg cccgcgacgg cgtgctcgaa ccacctgtcg tcgacgcgcg cgatgccgtt
     5641 gcccgagctg tagatcacgt cgatgtagta gcccccgcgg taagccttcg cgagccagtg
     5701 cggaaagggc cggtcgatcg agaagccggc ggcggcgagc gctccgagca ccggttcgag
     5761 gctggccgag tcgacgaaga cgtcgaagtc cttggtgaac cgctcgatgc cggtgtagcg
     5821 cgcaaacgcg taggcgccgc cgaccaggaa cggcaggccg agggcgtcca gcacgtcgag
     5881 gccctcgcga tacacccgct gggccgcctc cctctcgccg gccgtggccc gcacctccgg
     5941 cattctgttc acctcgcgaa acccgcgccc ggaggggtgc agaaatcgtg cctcacggga
     6001 cgtgcgaccc gaacgcctcg gaggcccctt cctgtctcgc cggcccgcgc accagcggga
     6061 gggtgaagag gcggaacggc ggacggtcgg ggtagtggcg ctgcagcagc gacatcccga
     6121 cgttgaagac ctgcacgccg ccgcgcgtcc gcccctcggg ccggccgtga tgggcgtggc
     6181 cgtgaaagac gacgttcacc cggtaccggt cgatcggctc ctcgaggcgg ctcgacccca
     6241 tgaagggaaa gatctcgagc ggctccccct cgaccgtcgc gcggatcggt gagtagtgca
     6301 ggaggacgac gcggcgctcg gcccggatgc ggcccagggc cgaaccaagc ttgagggtct
     6361 cctgcacggc gtcgtgcacg aactgcttca tggtggcctc gccccacggc tccagcgcgc
     6421 gccggtcgaa gccgccgccg aaccccttca cgcccgcgaa gcccaccccc atcagctcgt
     6481 acggctcgcc gtcgagcaca cgcacgccgg actcggccag gatggaggca acctcgccct
     6541 ccttgcccga ctcgtattcg tggttgccga gcaccgcgat gaccgggatc ttggcggcgg
     6601 ccgtcagctc gcgcacgagc agctgcgcct cgtcgggcag gccgtacgag gtcaggtcac
     6661 cagccagcag caggatgtcg gccgccgcgg cggcctgggt gagcactccc tggaggctgc
     6721 cctgcgagga tcgggtgaag tggatgtcgg cgagcgccgc cagcctcacc tccgtcttcc
     6781 gcggatccat cgggttcgag tccattcacg gcgctctctg caagttccgg ggccgaagct
     6841 cctggtcgcc ttccccgagc gctcagaagc tcttcaagcg cgagtcgccc ggcccggtag
     6901 cgcggcggcc catcgcgccg gcgcggcggg ctacaatctc gccggaggct tcccgtggaa
     6961 gacgtgaggg tctcgagctg gaacgagctg cacgaggtgc tgtacgaggg ctcgtggcag
     7021 gagcggctgg gccggttccg ctcgcggtat gcgttccgag ggcagtcgga ggtgggcgag
     7081 gagctgacga gcagcctcgc gcggcttggc gcgccgccgg aggtggaggg ccacctgctg
     7141 cgcaacttcc ggaagtacgc gcagcgcgag gcggcgcccg gcgactcgat ctggaactgg
     7201 ctggccgtcg cgcagcacca cggccttccc acgcgcctgc tcgactggac cttctcgccg
     7261 tacgtcgccc tgcacttcgc caccgagcac accgagaagt accacctcga cggggtggtc
     7321 tggcaggtcg actacgtggc gacgaaccag ctgctgccgg agcgcctgaa ggagatcctg
     7381 cgcgacgagg ggtcggacgt gttcacggcc gagatgctcg cgcgggcaag cgccaccctc
     7441 gccggcttcg accgcctcgc cgccggggag ttcgtcgcct tcttcgagcc cccctcgctc
     7501 gacgagcgca tcgtcatcca gttcgcgctg ttctccctga tgtcgggccc ccacgcgaat
     7561 ctcaaggcgt ggctcgacga gcacccccgg atgttccggc gcgtcgtgct gccggcgtcg
     7621 ctgaagtggg aggtgcgcga caagctcgac caggccaaca tcaccgagcg ggttctctac
     7681 cccggcctcg acggcctcag ccgctggctg cggcggtatt acatgcccag gcggcgctga
     7741 gcccggcgca cgcggacagc ccgcagcttc tccttgcctt tcgggccgct tggtgataat
     7801 tacttatcat tatcaacaag ccggggcggc ggcgaacgcg atgattggcg aagtcctgct
     7861 cgagagcgtg tacgacaccc tgcggatgct gccggtgctg gtgcccgtgt acatcctgct
     7921 cgagtacctc tcgcaccgcg aggtcccggg cctcgtccac cggttccggc tcggcggccc
     7981 cgccggcccg ctggtgggca cgttgctcgg catcatcccg cagtgcagca tgtcggtgct
     8041 cgtcacctcc ctgttcgcct cgggccgcgt gagcctcggc acggtgctgg ccacctacct
     8101 cgccacctcg gacgaggcgc tgccgctgat gatcgcggag gggcgccacg cgcgcgtggt
     8161 ggtcgccttc gtggcactca aggtcgccat tgccctcgcc gccggatacc tggccgacct
     8221 cgtgctcggg cggcggtacg ccgacctgcc gccgaccggg cgcgtgatcc acagccacac
     8281 cgagaaggcc tcctgggccg agatcgtccg ccacggcgtg cggcactcgg cggtcgtcgc
     8341 cgcctgggtg ttctcggcga ccttcctgct cggcctgata ctggcgtggg ggaccggccg
     8401 cgtctcctgg ctggccggat cgccgggatg gtcggtgccc cgcgtcgcgg gggtgacgct
     8461 gttcgggatg atccccaact gcgcggcgtc ggtcgcaatc accgaggcgt tcctgaaagc
     8521 cgggctgccc tttggcacgg ccgtcgcggg cctgtcggcg ggcgcggggt tcgggccgat
     8581 cgtgctgctg cgcgaggtgc ggctgcggca ggcgctgatc ctgctggggt ggctggcggc
     8641 catcgcgatg gctgccggcc ttgtcatcga tgcgtggtac ccgttctcga ttcccgtgag
     8701 gtagacatgg caccgctccg ggaaccctcc tcgcgggaca ccagccagcg gcgcgcgatt
     8761 cgctacgcgg tggagagcgc cggccggccg ctgtcagcgg ccgagatcct ggcttcggcc
     8821 cgccgcgagg tcccgggact cggcatcgcc acggtgtacc gggccgtcag gcgcatgcag
     8881 gaggaaggaa cgctcaggca ggtggacctg ccgggcgatg cccccaggta cgagtcgtct
     8941 ggcaaagggc accaccacca cttccactgc cgctcgtgct cgcgggtgta cgaggtggag
     9001 gcctgcccgg gcgagttcgt catgcaggct cccccgggct tcagcgtgga ggatcacgag
     9061 ttgatcctct acggccggtg ccccacctgc gtggcgccgc gaaggaccgg cgcgccacgt
     9121 tcccgcggca gccgcaccgt gtctcgcggg agggagcgat gatggcggag atcctggtcg
     9181 tcatctggct gctttcccag gctcgagacc cggcgctccc gttgccgggc gtcacgtctg
     9241 cggggccgca cctgtcgcag gagccggagg cgcaggagca gggggacggt caggccaggt
     9301 tcaatcccgc catctccgta atccccgatg tcgcgtacta caacgacaac cgcgacgggg
     9361 cggcgggagc gctcgtctcg ggggcggatg ggttcgcgcg ccacggggag ggaggaggca
     9421 acgcccatgg cagtgacctc gagcgcgggt tcaacctgcg ggaggtcgaa gtggccttct
     9481 cgggcgccgt cgatccgtac ttcgacgcgt cggccatcct cgcggtcgga gacggcgagg
     9541 tggaggccga ggaggtctac atccagacgc gcgcgctcgc gccggggctg cagcttcggg
     9601 ccggcaagtt ctacagcggg atcgggtacc tgaacgcaaa gcactcgcac cagtgggact
     9661 tcgtggatcg gccccttccc tactccacct gctcggcggc acgcttggcg aggtcgggct
     9721 gcaggcaacc tggcttccct cactccccgt ctacgtgctc atcggcggcg aactgctcca
     9781 gggcgccaac gaggcgttcg cgcagcagct ggcagcgtcg gaggaacagg gcttcgccgc
     9841 gaagggcggc ccgaggctct tcacgggatt cgtgaaggtc tcccccggcc tgggcgacag
     9901 ccacaccctg caggtgggga gctcgatcgc ccacacccgc tcgcaccagg agttcgccgt
     9961 cgagcacgga gacgaggagc cgctcgaggg gagcgggacc ctcgttggcg ccgacctcgt
    10021 ctaccagtac gactcgggca agcagtgggg agtcggcgac ctgacgctgc agggcgagta
    10081 cttctggcgc cggcacgacc tggccgtcgc gtcgagagcc ggcaggctgc agcgcgatcg
    10141 cacgcaggac ggcttctacg tgcagggggt gtacggcttc gcggcgcggt ggaaaggcgg
    10201 gggccggttc gatgccgccg gcctgacgaa ccagctgcac gttcgcgacg gcctcctgcg
    10261 cctcgaatcg gtgcggcggt acaccgccgt gctgacgttc atgccgaccg agttctcgcg
    10321 gctgcgcgtc cagtacaccc gcgccgattc accgatccgc gccgtccgcg agaagtcgaa
    10381 ccaactgtgg gtgcagctgc agatcagcct cggcgcccac ggcgcgcaca cgttctaagg
    10441 gagctgtggt gtgtcagcca ccaggtttgg attgagaatt cgcctcctcc tgccgttggt
    10501 cgccgcgttg gcgctggctg taccagcccc cgcccagaca atcgacgtcg tcgccacgac
    10561 ctcgagcatg ggagtgctgg cgcgagtcgt gggcggcccc gcggtgaagg taaccgttct
    10621 ggcgccgccc gaccgcgacg cccactacct gatcgcgcgt ccgagcatga tgctcgcgct
    10681 gcggcgggcc gacctgctcg ttgccgtcgg cgccgacctg gaagtcggct ggctgccggc
    10741 cgcgctgcgc agcgctggca accgcaaggt cctgccaggc cagcccgggt atttcgaggg
    10801 tgcggcccac gtcgacctgc tcgaaaaggg gcaggcggcc gatcggtcgc gcggcgacgt
    10861 tcacccggcc ggcaacccgc actactacat ggacccgctg cgcctgtcgg gcgccgccag
    10921 ggcgctggct tcgcacctcg cgaggctccg gccgggatcg gcgacggcct tcgccgccaa
    10981 cgccgacgcg ttcgcgaagg ccgcatccga gcgggtcgtc cagtggaagg cacgggctgc
    11041 gggcgcgccg ggcgtgctgt tgttccacaa ggacgtcaat tacctggccg acctgctggg
    11101 cgtgccaatc gtcggctacg tcgaaccggt ccccggcgtc ccgccgactg cgagccactt
    11161 gcgcgatctg gtgacgcggc tccaggggac gcgaggggtg atcctccacg ccgcgttcca
    11221 gccgtcggac gggccccagt accttgcgaa gaacctgggg tggccggtgc agcgactgca
    11281 gctcgagccg ccgccggacg ccgacctccc ggcctacctt gcgcacgtcg agcgctgggt
    11341 agcagccgtc gcgagcgcca agtgaacggc cacctgctgc tggaggtcga gaacctggtg
    11401 gccggctatc gcgatccggt cgtcggcccc gtctcgtttc ggctgaaccg cggggagatc
    11461 ctggggctcg cggggccgaa cgggtcgggc aagtccaccg tgcttcgcgc aatcatcggc
    11521 cgtgcgcgaa tcttctcggg caccgtgcgc cggggccacg acccgatcgc gacggcggtc
    11581 caggcacaac gccccgtgcg cctggctgag atgccaatca ccgggcggga gtaccttcgt
    11641 ctcaccggcg ccgcgcggcg ggccccgccc ccgcagctgg gcggcctcct cgacctccgc
    11701 ctcgacgaac tgagcggcgg ccagttccag ctgctgcacg tctgggcgtg cctggaatcg
    11761 ccggccggcc tggtactgct cgacgagccc gacaacaaca tggacccggg cgcgcgggcc
    11821 acgctcatcg aggtgctgca ccagtcgcgc acagcggggc gcggggtcct cgtcgtgagc
    11881 cacgagcacg acctggtcga gagcgtctgc tcgcgcatcg tcgaggtggc ggcatgagct
    11941 tcgccgacct cctccacccg atgttcctga tcccgttcgt caacggcctg ctgctggcga
    12001 tcctgctgcc gctcgtcggc gcccacgtgc ggctgcgcga ggagtggctg gcgtcgctcg
    12061 ggctggcgca ggcagcggca gccggggtga tggtcggggc cttcctggac gcgccaatcg
    12121 cggtggtggc cctgacggcc gcggttgtcg cggccctggc gaagagcttc ttcggcccca
    12181 aggggaacga atcgtacgcc ctgatgctgc ttctgggatg gaccgtggcg ctgctgctgg
    12241 cggccaacag cgcccacggc gacgatctct cgcacgcgct gaccgagggg cagctctact
    12301 tcaccggcgg gagccacctg caggcgctcg tcgcggtcgg cctcgtgcag ttcggcctgc
    12361 tgccgtggct ggcgcctcgc ctgctgctcg cacgcttctt tcccgatcac ttcgccgcca
    12421 acgggcggcc caacccgcac cacgagatcg tcttcgacgt gctcgtcgcg gtgaccctgg
    12481 cgctcgccgc caccgcgatc ggcgtgatgg cggccttcgc ggtcgtcttc ctggcgccct
    12541 ggatcgcctt ccgcttcgcg cgcggctggt tcaagacgat cgcgctcggc atcgccgtca
    12601 gcctggccgc
//
LOCUS       DAOPQX010000072        12553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5834_length_12553_cov_4.573612, whole genome shotgun sequence.
ACCESSION   DAOPQX010000072 DAOPQX010000000
VERSION     DAOPQX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12553
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5834_length_12553_cov_4.573612"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PKK95_05810"
     CDS             complement(<1..980)
                     /locus_tag="PKK95_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOC17761.1"
                     /translation="MMPHRIRTRPPLGQAGLPPRASDPDAVRAFLEDAAHFPGGQAEA
                     VVFPRSEAEVAETVRTGVRVLPVGAQSSVTGGATPLGGVVLSTSKMNAVLEVGRDEVR
                     VQAGLPLVTLKADLAARGLYYPPAPTYDGAFVGGTVSTNASGAATFKYGSTRAWVRGL
                     TVVLASGAVLELERGQARAHPDGYFEIEEDGRLVRVMVPTYTMPRVAKRSAGYHAEPG
                     MDLVDLFVGAEGTLGVVVEARLGVVSPAPALCVVLAFFRSEAAALDLAGRLRRESRET
                     WRTRDPRGLDVSAIEMLDAGSLALLHEDGADAKNGIELPPGTETALIVQV"
     gene            complement(1070..2446)
                     /locus_tag="PKK95_05815"
     CDS             complement(1070..2446)
                     /locus_tag="PKK95_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17762.1"
                     /translation="MLHPAARLPAACLVASLSCALIAQPAPRAPGAVPQRSEPVSPSV
                     ARRHPGFPARSGIVRAAGRTWADAGGEFYPLGATLFWALRGWKFERARLERNLDFLAR
                     EGWDYVRILGEVGWAGNEIDPRWDDYAQVLGEFVDTAYGTYGLRTELTLIGGGTGTRP
                     IDLARKVVPVLAPRTSKIMNLEVANEAFQNYPDEEALFEALRHLRAHLPNLVAVTSPR
                     GTPQATIATMKRYISSGATLATVHLSRPYDAREGAWRHVGLGWSYRGLPFPIAHNEPG
                     GPRSSVAQYDQPIHLVMSRAVGIMSGIGAYVLHNGAGIAGRVDKRRDRPPNLWEVPRI
                     GEIMRALRRLDSHLPAGLGNGRHYSTGAAGHPLPVTGRGAGTEPGHDVIRADAVDLGN
                     RFVVMPIGVKGRLTMTAREACTVEVVDPATGEAFRQTLRAGEALQLVPQSLDPTGLGA
                     FIIVGTRE"
     gene            2497..2781
                     /locus_tag="PKK95_05820"
     CDS             2497..2781
                     /locus_tag="PKK95_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOC17763.1"
                     /translation="MPLRPRFGAFVALAALLAAPAGAWAQAARSAARMPMEEFKRLLQ
                     ESAVVVVDVRDAASYAAGHIPGALSVPLDSVAKHAPKLKELKKPIVTYCA"
     gene            complement(2891..4387)
                     /locus_tag="PKK95_05825"
     CDS             complement(2891..4387)
                     /locus_tag="PKK95_05825"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC17764.1"
                     /translation="MHSGARAAMVAALLAAVALVALVLAADRPRGAGRLQSDRELDRE
                     ARAILEELVSINTVEPRGSTEAALAMAARFRAAGFPDEDIHLVGPVQHKQNLVVRLRG
                     TAPEAKPLLLLAHLDVVEARREDWSSDPFTLVEKDGYLYGRGTTDMKDMAACWVVTLL
                     DMKRRGIRPLRDVVLALTADEEGGGANGVDWLVRTRRELIEAAFCLTEGGGGQIHDGR
                     YTVYNLQAAEKVYLTFRLEATDTGGHSALPRRGNPIVRLAAALGRLDSFQFPARLNEV
                     TRSFFARMAEVEGGSAGLDMRRLAEAPPPDVGPSSPPLAVSRSVPAADAAGAERRLSR
                     QPYFNALLRTTCTATRIEGGHAENALPQRATAWVNCRLLPDDSADQVEQALVEAIRDA
                     EVRVTRMGQPSPGPAAPLLPEVVQAVEGAVAERWPHVIVVPTMLTGATDGRILRLAGI
                     PTYGLGAFEDVSDSRAHGRDERIGIRQFREERALLAAIVTRLSGAPSR"
     gene            4533..6005
                     /gene="argH"
                     /locus_tag="PKK95_05830"
     CDS             4533..6005
                     /gene="argH"
                     /locus_tag="PKK95_05830"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOC17765.1"
                     /translation="MGFAPEYAESVLAENFADARRLFLGPLLAIHRAHLVMLAERGIL
                     SSADARALGRALSALPLDELSAAAYDGTSEDLFFHVNRLLERSCGPDLAGRLHTARSR
                     NDIDMTMYRMVLRGRLLELLDGTLWLRGVLLELAGRHRETILALHTHTQPAQPSTVAH
                     YLIGAVEQLERDVVRLRAAYAVVNRSPLGACAITGTGFPIDRALTGALLGFDAATGNT
                     YGSIAAVDHLLEAAGAAATLAVGLGRFLQDLLLWSTSEFGYLRLGEGFVQGSSIMPQK
                     RNPVALEHARAIASRALGEAIAVLVATHNTPFGDVVDTEDDLQPLVDRLFHDARRAVR
                     VTAAALSTAEFDVAALEARASEGGTTLTELADTLVRERGLPFTQAHAIAARVAAERAS
                     DASAPLAPILAAVSTELTGSSIDYDEERLSALLGARYFVSVRTTAGGPAPRRTAEALA
                     ASLDLLATDRRWLEEARERLAEAEARRNRQFAALESGKQG"
     gene            6126..7823
                     /locus_tag="PKK95_05835"
     CDS             6126..7823
                     /locus_tag="PKK95_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOC17766.1"
                     /translation="MHLLDWLIVGAYLGWLIRDGLRRTHSTNEIDGYLLAKRSLPWWA
                     AGLSVMATQLSAITLVGTTGQGYSDGMRFVQFYFGLPIAMVILSLTVVPFFYRAKVYT
                     AYEFLERRFDAKVRALATLLFLGGRSLSSGVVIAAPAVILSVVLGWNLSLTVLAIGAP
                     TVVYTMFGGVQAVAWTDVKQMAVIVAGVAAAVVALVLGLPPEVGLTDSLHLAGAVGRL
                     KTLDFSFSLTERYTVWSGTLAGLFLMLSYFGCDQSQVQRYLTARSIDESRRSLLMSAY
                     FKIPLQAFILLTGVLVFAFYVFTQPPMLFNPVHQAGVESSNRAADYRALDRRFGEVFE
                     ERRKAAHAYAEASDRDDTAAAREARAAFARANSEMAATRGEAAALVREVTGDRLYEDV
                     NYVFPTFITTHLPTGLIGLMIAAIFAAAMSSAAGEMNSLATATVIDVYRRHVRVDATD
                     AHYLAVSKAATAFWGLVACMFALYAARAGSLIEVVNMVGSLFYGTLLGAFVLAIATRR
                     ATGTGTFVGMIAGFAAVIYCAWTGSVSYLWYNVVGTVVVVVVGLLVSAVTSPRPASR"
     gene            7848..8609
                     /locus_tag="PKK95_05840"
     CDS             7848..8609
                     /locus_tag="PKK95_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOC17767.1"
                     /translation="MSGTTTPAASRPSQDAIDRLMWKLRLKTPLIAVYDAPASDEFAP
                     LAEAEPETCCFAFYDRWVGGETLVLRRGGGGCKGAFRALGLEKGGDPNVIAHMLTDGV
                     GAPKAEGLKASADIALAAIESGKTPTPSFDTVLVGPLRVDQWPAVRSVTFLVDPDRLA
                     AVMTLAAFWSPDDVVSAPFGSGCSFLWKSLGQGEGPLAIIGGTDVAMRRFLPTAILTL
                     TVAPSHFVKMLAAPEDSFLDRDWWNELMDTRGRWA"
     gene            8606..9031
                     /locus_tag="PKK95_05845"
     CDS             8606..9031
                     /locus_tag="PKK95_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17768.1"
                     /translation="MKAGLLAALLLAVVFGCVAAGLQPGNAAATEPASESAAVAGEAQ
                     VKLGEKPLDPAEARQRAVERAKTHLSDELDIPIAEIELVSVKAVTWSDTSLGCRQPDR
                     MYAQVLVDGHEVVLEARGQKHELHLGPKNVVICTTKKQG"
     gene            9248..10693
                     /gene="sufB"
                     /locus_tag="PKK95_05850"
     CDS             9248..10693
                     /gene="sufB"
                     /locus_tag="PKK95_05850"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOC17769.1"
                     /translation="MSTAERTIEELATQEYKYGFVTDVEADAVPRGLNEDIVREISRR
                     KGEPEWLLEWRLKAYRAWQGMQEPRWGNVKHDPIDYQDIIYYSAPKPKKALGSLDEVD
                     PEIRRTFEKLGIPIEEQKALAGVAVDAVFDSVSVATTFREKLAELGIIFCSFSEAVRN
                     HPDLVREYLGSVVPHTDNFYAALNSAVFSDGSFAYVPKGVRCPMELSTYFRINAQNTG
                     QFERTLIVADEGAFVSYLEGCTAPMRDENQLHAAVVELVALDRATIKYSTVQNWYPGD
                     KEGRGGIYNFVTKRGHCRGADSKITWTQVETGSAITWKYPSCILQGDNSIGEFYSVAV
                     TNNFQQADTGTKMIHLGKNTRSTIVSKGISAGRGQNTYRGMVKIGRNAENARNYSQCD
                     SLLIGDKCGAHTFPYIEIKNTSAQMEHEASTSKIGEDQIFYCRQRGLSTEDAVNMIVS
                     GFCKEVFRELPMEFAVEAQKLLGVSLEGSVG"
     gene            10721..11485
                     /gene="sufC"
                     /locus_tag="PKK95_05855"
     CDS             10721..11485
                     /gene="sufC"
                     /locus_tag="PKK95_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOC17770.1"
                     /translation="MLEIKNLHARVGGNAILKGIDLAVATGEVHAIMGPNGSGKSTLA
                     GVLAGRGDYEVTEGSVAYQGLDLLGLDPEERAREGLFLAFQYPVEIPGVNNAYFLKAA
                     LNALRKHRGEPELDAMDFMALVRDRMKVLDIEPSLLNRPVNEGFSGGEKKRNEIFQMA
                     VLEPRLAILDETDSGLDIDALKVVAHGVNALRRPDRSTIVVTHYQRLLNYIVPDFVHV
                     LSEGRIVRSGGKELALELEERGYGWIEDGEPVGGRA"
     gene            11485..>12553
                     /locus_tag="PKK95_05860"
     CDS             11485..>12553
                     /locus_tag="PKK95_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOC17771.1"
                     /translation="MSTAAAGSSPVSQFEASYAAAAERHALEPAWLQAVRARAASAFS
                     RLGFPTTRDEDWRFTNVAPIAQGGFVPAEATEEAAAEDRDRYLLPGLRGPVIVFVDGR
                     FAPALSRLDGLPPGVEAGSLFEGDRAHRLQRHLTAHAPFDQRSFTSLNTSLFPDGAII
                     EIAPRTVVDEPIQLLFLSSGREGTASFPRALVVAGEASQVALVESFGSIGDGRAFTNA
                     VTELVAGPAAVVDHYRLQRESARGYHVGLTQIRVGRSANVSSHNAALGGLIARHDAVV
                     VLADEGAESTLDGVYLAGGQALVDNHTEVDHVMPHGTSHELYKGILGGRARAVFNGRI
                     RVRPDAQKTDAKQTNKTLLLSN"
BASE COUNT         1859 a         4396 c         4433 g         1865 t
ORIGIN      
        1 tccacctgca cgatgagcgc ggtctcggtg cccgggggca gctcgatgcc gttcttggca
       61 tcggccccgt cctcgtgcag cagggccagc gagccggcgt cgagcatctc gatcgccgac
      121 acgtcgagcc cgcgagggtc tcgcgtccgc cacgtctcgc gcgactcgcg ccgcagccgt
      181 cccgccagat ccagggctgc cgcctcggag cggaagaacg ccagcaccac gcagagagcg
      241 ggcgccggcg acaccacgcc gagccgcgcc tcgaccacga cgccgagcgt gccctccgcg
      301 ccgacgaaca ggtcgaccag gtccatgccg ggctcggcat gatagccggc cgagcgcttg
      361 gccacgcggg gcatggtgta ggtgggcacc atcacccgca ccagcctccc gtcctcctcg
      421 atctcgaagt agccgtcagg gtgagcgcgg gcctggcccc tttcgagctc cagcacggcg
      481 cccgacgcga gcacgactgt cagccccctc acccacgccc gcgtgctgcc gtacttgaag
      541 gttgcggccc ccgaggcgtt ggtggagacg gtgccgccga cgaaggcgcc gtcataggtg
      601 ggcgccggcg ggtagtagag gccgcgtgcg gcaaggtcgg ccttcaaagt gacgagcggc
      661 agccccgcct gcacccgcac ttcgtcgcgt cccacctcga gcacggcgtt catcttcgag
      721 gtggacagga cgaccccgcc gagcggcgtg gccccccccg tcaccgacga ttgcgcgccg
      781 acgggcagca cgcgaacgcc cgtccgcacc gtttccgcga cctcggcctc cgatcgcggg
      841 aacacgaccg cttcggcctg gccgcccggg aaatgcgcag cgtcctcgag gaaagcacgc
      901 acggcatcgg gatccgacgc gcgcggcggc aggcccgcct ggccgagcgg cgggcgggta
      961 cgaatgcggt gcggcatcat tcctcacccc gatgatagcg ctgtcaccag gccatccgtt
     1021 cgtctccgtg ctctgtggtg aaccgggttt ggtgtgccgc gccgtcgggt cactcgcgcg
     1081 tgccgacgat gatgaaggcg ccgagtccgg tgggatcgag actttggggc accagctgca
     1141 gggcctcacc ggcgcgcagc gtctggcgga aggcctcccc cgtcgcggga tcgacgactt
     1201 cgaccgtgca ggcctcacgc gcggtcatgg tgagccggcc cttgaccccg atcggcatca
     1261 cgacgaagcg gttgccgaga tcgacggcgt cggcccggat cacgtcgtgg cccggctctg
     1321 tgcctgctcc ccggccggtc accgggagcg ggtggccggc ggcgcccgtc gagtagtggc
     1381 ggccgttgcc gagcccggcg ggcaggtgcg aatccaggcg ccgcagggca cgcatgatct
     1441 cgccgatccg cgggacctcc cagaggttcg gcggccgatc ccggcgcttg tccacccggc
     1501 ccgcgatgcc cgcgccgttg tggagcacgt atgcgccaat cccgctcatg atcccgaccg
     1561 cccggctcat cacgaggtgg atgggctgat cgtactgcgc caccgacgag cgcgggcccc
     1621 ccggttcgtt gtgggcaatg ggaaaaggga gaccccgata gctccatccg agccccacgt
     1681 ggcgccaggc cccttcgcgg gcgtcgtacg gccgcgagag gtgcacggtg gcgagcgtgg
     1741 cgccgctgct gatgtaccgc ttcatcgtcg cgatggtcgc ctgtggggtt cccctcggcg
     1801 acgtgacggc aacgaggttc ggcaggtgtg cccgcaggtg gcgcagcgcc tcgaacagcg
     1861 cctcctcgtc cgggtagttc tggaacgcct cgttggcgac ctcgaggttc atgatcttcg
     1921 aggttcgcgg cgcgagcacc gggaccacct tacgcgcgag gtcgatcggc ctcgttcccg
     1981 tgccgcctcc gatcagggtc agctcggtcc gcagtccgta ggtgccgtag gccgtgtcga
     2041 cgaactcccc gagcacctgc gcgtagtcgt cccagcgcgg gtcgatctcg ttccccgccc
     2101 agccgacctc gcccaggatg cggacgtagt cccacccttc gcgagccagg aagtcgaggt
     2161 tccgctccag cctcgcccgc tcgaacttcc agccgcgcag ggcccagaag agcgtcgcgc
     2221 ccagcgggta gaactcgccg ccggcgtccg cccacgttcg gccggctgcc cggacgatgc
     2281 cggatcgggc ggggaacccc gggtgccggc gcgcgacact cgggctgact ggctcactcc
     2341 tctgcggtac cgcccctggc gcccggggcg cgggctgggc gatcagcgcg caagagagcg
     2401 acgcgacgag gcaggccgcc ggcaggcggg ccgcggggtg cagcatggct gtactgccat
     2461 tctaccggtg cgggtatgat ggctgaagga gagcgtatgc cgttacgacc gaggttcgga
     2521 gcgttcgtcg cactcgcggc gctgctggca gcgccggccg gcgcgtgggc gcaggctgca
     2581 cgatccgccg cccgcatgcc gatggaggag ttcaagcggc tcctgcagga gagcgccgtc
     2641 gtggtcgtgg atgtccgcga tgccgcgtcg tacgcggccg ggcacatacc cggggcgctg
     2701 tcggtgccgc tggacagcgt cgcgaagcat gcgcctaagc tcaaggagct gaagaagcca
     2761 atcgtcacct actgtgcttg accgagcgag caaacgagcg cccgtgcggc gctcaacctc
     2821 gcggcgctcg gcgtggagaa cgtgcgcgcg ttgaagggcg gctggcaccg ctgggtggcc
     2881 gagaaggggg ctatcgagaa ggggcccccg aaagcctggt gacgatggcc gcgaggaggg
     2941 cccgctcctc gcggaactgc cggatgccga tgcgttcgtc acgtccgtgc gcgcggctgt
     3001 ccgagacgtc ctcgaacgcc cccagcccgt aggtcgggat gccggcgagc cgcaggatgc
     3061 ggccgtcggt cgccccggtc agcatcgtcg gcaccacgat cacgtgcggc caccgctcgg
     3121 cgaccgcgcc ctcgaccgcc tgcaccacct ccgggagcag cggggcagcg gggcccggac
     3181 tcggttggcc catcctcgtc accctcacct ccgcatcccg tatcgcctcg accagcgcct
     3241 gctcgacctg gtctgctgag tcgtcgggca gcagccggca gttcacccaa gcggtggctc
     3301 tctggggcaa tgcgttctcg gcgtggcccc cctcgatcct cgtggccgtg caggtggtgc
     3361 ggagcagggc gttgaagtac ggctgccgcg acaggcggcg ctccgcgccg gcggcatcgg
     3421 ctgccggcac cgaacgcgaa acggcgaggg gcggcgatga cgggccgacg tcgggaggcg
     3481 gggcctcggc caggcgccgc atgtcgaggc cggccgaccc gccctccacc tccgccatgc
     3541 gggcgaagaa cgagcgcgtc acctcgttca ggcgggccgg gaactgaaac gaatcgaggc
     3601 ggccgagcgc cgccgccagc cgaacgatcg ggttcccccg tcgtggcagc gcgctgtggc
     3661 cgcccgtgtc ggtggcctcg agcctgaacg tgaggtacac cttctcggcg gcttggaggt
     3721 tgtagaccgt gtaccggccg tcgtggatct gcccgccgcc tccctcggtc aggcagaacg
     3781 ccgcttcgat cagttcgcgg cgcgtcctca ccagccagtc aacgccgttg gcgccgcctc
     3841 cctcctcgtc cgccgtcagc gccagcacca cgtcgcggag cggccggatc ccgcgacgct
     3901 tcatgtcgag cagcgtcacc acccagcagg ccgccatgtc cttcatgtcg gtcgtgccgc
     3961 ggccgtagag atacccgtcc ttctcgacaa gggtgaacgg atcggacgac cagtcctcgc
     4021 gccgggcctc gacgacgtcg aggtgggcga gcagcaggag cggcttggcc tccggggcgg
     4081 tcccccgcag gcggactacc aggttctgct tgtgctgcac cgggcccacg aggtggatgt
     4141 cctcgtcggg gaagccggcg gcgcggaaac gggcggccat cgcgagtgcg gcctctgtcg
     4201 acccgcgcgg ctccaccgtg ttgatcgaga ccagctcctc gaggatggcg cgcgcttccc
     4261 ggtcgagctc gcgatccgac tgcaggcggc ccgccccgcg cggccgatcg gcagcgagga
     4321 cgagcgcgac cagcgcgacc gcggcgagca gcgcggcgac catggcggcg cgggctcccg
     4381 aatgcatgcg gagattatag ctacgcgatc tgccgccggc cggccagctc gcgcgcgaga
     4441 cggtcgcgca ggaaggcgag taccgcggcc tcctcgggtt cgaggccccg cagcccggca
     4501 ccacacggcc ggcgctactc tatactcgaa gcatgggctt cgcccccgag tacgcggagt
     4561 cggtcctcgc ggagaacttc gcggacgcca ggcgcctctt cctcggccct ctgctggcga
     4621 tccaccgcgc ccacctggtc atgctggccg agagagggat cctctcgtcc gccgacgcgc
     4681 gcgccctggg ccgcgcactc tcggcccttc ctctcgacga gctgtcggcc gcggcgtacg
     4741 acgggacgtc ggaagacctg ttcttccacg tgaaccgcct gctcgagcgc tcgtgcggcc
     4801 ccgacctcgc ggggcggctc cataccgcgc gcagccggaa cgacatcgac atgacgatgt
     4861 accggatggt gctgcgggga cggctgctcg agctgctcga cggcacgctg tggctgcgcg
     4921 gggtactgct cgagctggcc gggcgtcacc gcgagacgat cctcgcactc cacacgcaca
     4981 cccagccggc ccagccctct accgtcgcgc actacctgat cggggccgtc gagcagctcg
     5041 agcgcgacgt ggtgcggctg cgggcggcct acgcggtggt caaccgctcg ccgctcgggg
     5101 cctgcgcgat caccgggacc ggctttccga tcgatcgtgc gctcaccggc gcgctgctcg
     5161 ggttcgacgc ggcgaccggc aacacctacg gcagcatcgc cgccgtcgat cacctgctcg
     5221 aggccgcggg cgccgcggcg acgctcgccg tcgggctcgg ccgcttcctg caggacctgc
     5281 tcctctggtc caccagcgag ttcggctacc tgaggctggg cgaggggttc gtgcagggca
     5341 gcagcatcat gccgcagaag cggaacccgg tcgcgctcga gcacgcgcgc gcgatcgcga
     5401 gccgggcgct cggcgaggcg atcgcggtgc tcgtggcgac gcacaacacg cccttcggcg
     5461 acgtcgtcga caccgaggac gacctgcagc ccctggtcga ccgcctcttc catgacgcgc
     5521 ggcgcgcggt gcgtgtgacc gcggccgcgc tctcgaccgc cgagttcgac gtggccgcgc
     5581 tcgaggcccg cgcctcggag ggcggcacca ccctcaccga gctggccgac actctcgtgc
     5641 gcgagcgcgg gctgccgttc acccaggcgc acgccattgc cgcgcgcgtg gccgcggaac
     5701 gagcgagcga cgcctcggct cccttggcgc cgatcctggc cgccgtctcg accgagctga
     5761 cgggctcgtc aatcgattac gacgaggaac gcctgtcggc gctgctcggc gcgaggtact
     5821 tcgtgagcgt gcgtacgacc gctggcgggc cggccccccg gcgaacagcg gaggccctgg
     5881 cggcctcgct cgacctcttg gccaccgacc ggcgctggct ggaggaagcg agggagcgcc
     5941 tggcggaggc cgaggcccgg cgcaaccggc agttcgcggc cctcgagagc ggcaagcagg
     6001 gttgaaggag cacggcatgg acgagcctgt tcgaacctgg tcctacgtgg gcgtcctcgt
     6061 catcgaggcg ctggtggtcc ttggactgtg ggccttcggg cggtacttca gcgcgtgagc
     6121 tgcccatgca cctgctcgac tggctcatcg tcggcgccta cctgggctgg ctgatccgcg
     6181 acggcctgcg aaggacccac tccaccaacg agatcgacgg ctacctgctg gccaagcgga
     6241 gcctgccctg gtgggccgcc gggctgtcgg tcatggcgac gcagctgtcg gcgatcacgc
     6301 tggtcggcac gaccgggcag gggtactcgg acgggatgcg gttcgtgcag ttctacttcg
     6361 gcctgccgat cgcgatggtc atcctctcgc tcaccgtggt gcccttcttc taccgggcca
     6421 aggtctacac ggcgtacgag ttcctcgagc gccggttcga cgcgaaggtg agggcgctgg
     6481 caacgctgct gttcctcggc gggcgcagcc tgtcgagcgg ggtggtgatc gcggcgcccg
     6541 cggtgatcct ctcggtggta ctcggctgga acctctccct gacggtgctc gcaatcggcg
     6601 cgcccactgt cgtctacacc atgttcggcg gcgtgcaggc ggtggcctgg accgacgtga
     6661 agcagatggc ggtgattgtc gccggcgtgg ccgccgcggt ggtcgccctc gtactcgggc
     6721 ttccccccga ggtggggctg accgactcgc tgcaccttgc gggcgcggtg ggccggctga
     6781 agacgctcga cttctccttc agcctgaccg agcggtacac ggtgtggtcg ggaacgcttg
     6841 caggcctgtt cctgatgctg tcgtacttcg gctgcgacca gagccaggtg cagcgctacc
     6901 tcaccgccag gtcgatcgac gagagccgcc gctcgttgct gatgagcgcc tacttcaaga
     6961 tcccgctcca ggcgttcatc ctgcttaccg gcgtcctggt gtttgccttc tacgtgttca
     7021 cgcagccgcc gatgctgttc aacccggtgc accaggccgg ggtcgagtcg agcaaccggg
     7081 cggccgacta ccgggcgctc gaccggcggt tcggcgaggt tttcgaggag cggcgcaagg
     7141 cggcgcacgc ctacgccgag gcgagcgacc gcgacgacac cgccgcggcc cgggaggccc
     7201 gggccgcttt cgcccgggcg aactccgaga tggctgccac gcgcggcgag gcggccgccc
     7261 tggtcaggga ggtcacgggc gaccggctgt acgaggacgt caactacgtc ttcccgacct
     7321 tcatcaccac gcacctcccg acgggcctca tcggtctgat gatcgcggcc atcttcgcgg
     7381 ccgcgatgtc gtcggccgcc ggcgagatga actcgctcgc gactgccacc gtgatcgacg
     7441 tgtaccggag gcacgtccgg gtcgatgcga ccgacgccca ctacctcgca gtctcgaagg
     7501 cagcaaccgc cttctggggg ctggtggcct gcatgttcgc gctgtacgcg gcgcgcgcgg
     7561 gctcgctgat cgaggtggtg aacatggtgg ggtcgctctt ctacgggacg ctgctcggcg
     7621 ccttcgtcct cgcgatcgcc acccgccgag ccaccggtac tggcaccttc gtcgggatga
     7681 tcgcgggctt cgccgccgtc atctactgcg cctggaccgg cagcgtctcg tacctctggt
     7741 acaacgtggt cggcacggtg gtcgtggtcg tggtcggcct gctggtcagc gcggtcacct
     7801 ccccgcgccc tgcttcgcgg tagaatccgg gtacaccggg aggaaacatg agcggcacga
     7861 caacacccgc ggcctcgcgc ccgtcgcagg acgcgatcga tcgcctgatg tggaagctcc
     7921 gcctcaagac gccgctcatc gccgtgtacg acgcgccggc gtcggacgag tttgcgccgc
     7981 ttgccgaagc cgagcccgag acctgctgct tcgctttcta cgaccgctgg gtgggaggag
     8041 agacgctcgt gctgcggcgc ggcggcggcg gctgcaaggg cgccttccgc gcgctcggcc
     8101 tcgagaaggg gggcgacccg aacgtgatcg cgcacatgct gaccgacggt gtgggcgcgc
     8161 ccaaggccga aggcctgaag gcctcggccg acatcgccct cgccgccatc gagtcgggca
     8221 agacgccgac gccgagcttc gacacggtgc tcgtcgggcc gctgcgcgtc gatcagtggc
     8281 cggccgtccg cagcgttacc ttcctcgtcg accccgaccg gcttgccgcc gtgatgacgc
     8341 ttgccgcctt ctggtcgccc gacgacgtgg tgtcggcgcc gttcgggtcg ggctgcagct
     8401 tcctgtggaa gtcgctcggg cagggcgagg ggccgctcgc gatcatcggc ggcaccgacg
     8461 tggccatgcg gcgcttcctg ccgaccgcca tcctcacctt gacggttgcg ccgtctcact
     8521 tcgtgaagat gctggcggca ccggaagatt cgttcctcga ccgcgactgg tggaacgagc
     8581 tgatggacac tcgcgggcgg tgggcatgaa ggccgggctc cttgccgcgc tgctgctcgc
     8641 cgtcgtgttc gggtgcgtgg cggccggcct ccagcccggg aacgcagcgg ccacggagcc
     8701 ggcctcggaa agcgcggccg ttgccgggga ggcgcaggtg aagctcggcg agaagccgct
     8761 cgatcccgcc gaggcccgtc agcgggccgt cgagcgggcg aagacccacc tgtcggacga
     8821 gctcgacatt ccgattgccg agatcgagct cgtgtcggtc aaggccgtca cctggtccga
     8881 cacgagcctg ggctgccggc agcccgaccg catgtacgcc caggtgctgg tcgacgggca
     8941 cgaggtggtg ctcgaagccc ggggccagaa gcacgagctg cacctcgggc cgaagaacgt
     9001 ggtgatctgc accacgaaga agcaaggctg acggactcct cggcaccccc gccacgccaa
     9061 ccctccctca ccacggatca gctttatata cgacttgttt tgtccgcgat gtgatatatt
     9121 ggagactcaa acggtcggag taaagcggct gaccgggagc gggcccccaa tttcggtcag
     9181 ctcttccttt gctccatcgg ccgcggggct ccgggctggg ccgcgcggcg cgccccgggg
     9241 agagccgatg agcactgcag aacgaaccat cgaagagctg gccacccagg aatacaagta
     9301 cggcttcgtc accgacgtcg aggccgacgc ggttccccgc ggcctgaacg aggacatcgt
     9361 ccgcgagatc tcgcggcgca agggcgagcc cgagtggctg ctcgagtggc ggctgaaggc
     9421 gtaccgcgcc tggcagggga tgcaggagcc gcgctggggg aacgtcaagc acgatcccat
     9481 cgactaccag gacatcatct actactcggc gcccaagccg aagaaggcac ttggcagcct
     9541 cgacgaagtg gaccccgaga tccggcggac cttcgagaag ctggggatcc ccatcgagga
     9601 gcagaaggcg ctggccgggg tggccgtcga cgcggtcttc gacagcgtgt cggtggcgac
     9661 gaccttccgc gagaagctgg ccgagctggg gatcatcttc tgctcgttct ccgaggcggt
     9721 gcgcaaccat cccgacctgg tgcgcgagta cctcggcagc gtcgttccgc acaccgacaa
     9781 cttctacgcg gcgctgaact cggcggtctt cagcgacggg tcgttcgcgt acgttccgaa
     9841 gggggtgcgc tgcccgatgg agctgtccac ctacttccgg atcaacgcgc agaacaccgg
     9901 gcagttcgag cgcacgctga tcgtcgcgga cgagggggcg tttgtgagct acctcgaggg
     9961 gtgcacggcg ccgatgcgcg acgagaacca gctgcacgcg gccgtcgtcg agctggtggc
    10021 gctcgaccgc gcgaccatca agtactcgac cgtgcagaac tggtaccccg gtgacaagga
    10081 aggccggggc ggcatctaca acttcgtgac caagcggggc cactgccgcg gggcggactc
    10141 gaagatcacc tggacgcagg tcgagaccgg ctcggcgatc acctggaagt acccgagctg
    10201 catcctgcag ggcgacaact cgatcggcga gttctactcg gtggccgtga ccaacaactt
    10261 ccagcaggcg gacaccggca ccaagatgat ccacctgggg aagaacacgc gcagcacgat
    10321 cgtgtcgaag gggatctcgg ccggccgcgg gcagaacacg taccgcggga tggtgaagat
    10381 cggccgcaac gccgagaacg cgcgcaacta ctcgcagtgc gactcgctgc tgatcggcga
    10441 caagtgcggg gcgcacacct tcccctacat cgagatcaag aacacctcgg cgcagatgga
    10501 gcacgaggcg tcgacctcga agatcggcga ggaccagatc ttctactgcc gccagcgggg
    10561 gctttccacc gaggacgcgg tgaacatgat cgtgagcggg ttctgcaagg aagtgttccg
    10621 cgagctgccg atggagttcg cggtcgaggc gcagaagctg ctgggagtga gcctcgaggg
    10681 gtcggtgggg taggtgacgc aaccgcaaga cgagggacga atgctcgaga tcaagaacct
    10741 gcacgcccgg gtgggcggca acgcgatcct gaaggggatc gacctcgccg tggccaccgg
    10801 cgaggtgcac gcgatcatgg ggccgaacgg gtcgggcaag agcacgctgg ccggcgtgct
    10861 ggccgggcgg ggcgactacg aggtcaccga gggaagcgtg gcctaccagg gcctcgacct
    10921 gctggggctc gaccccgagg agcgcgcccg tgagggcctc ttcctggcct tccagtaccc
    10981 cgtcgagatt cccggcgtca acaacgccta cttcctcaag gcggccctca acgcgctgcg
    11041 caagcaccgc ggcgagcccg agctggacgc catggacttc atggcgctgg tccgcgaccg
    11101 gatgaaggtg ctcgacatcg agccctcgct gctcaaccgc ccggtgaacg agggattctc
    11161 gggcggcgag aagaagcgga acgagatctt ccagatggcg gtgctcgagc cgaggctggc
    11221 gatcctcgac gagaccgact cgggcctcga catcgacgcg ctcaaggtcg tcgcgcacgg
    11281 cgtcaacgcc ctgcgccgcc ccgaccgctc gaccatcgtc gtcacgcact accagcggct
    11341 gctcaactac atcgtccccg acttcgtgca cgtgctctcg gaggggcgca tcgtgcggtc
    11401 gggcggcaag gagctggcgc tcgagctgga ggagcggggc tacggatgga tcgaggacgg
    11461 cgagccggtc ggggggcggg cgtgatgtcg accgccgccg ccggttcgtc ccccgtgtcg
    11521 cagttcgaag cgtcgtacgc ggccgcggcc gaacggcacg ccctcgagcc cgcgtggctg
    11581 caagccgtgc gggcgcgggc cgcgagcgcc ttctcacgcc tcgggttccc gaccacgcgt
    11641 gatgaggact ggcgcttcac caacgtcgct cccatcgccc agggcgggtt cgtcccggcg
    11701 gaagcgacgg aggaggcggc cgccgaagac cgcgaccgct acctgctgcc ggggctgcgg
    11761 ggcccggtca tcgtcttcgt ggacggccgg ttcgcgccgg cgctctcgcg cctcgacggc
    11821 cttccgcccg gcgtggaggc cgggagtctc ttcgagggcg accgggcgca ccgcctccag
    11881 cgccacctga ccgctcacgc gccgttcgac cagcggtcgt tcacctcgct caacacgtcg
    11941 ctgttccccg acggcgcgat catcgagatt gcgccaagga cggtggtgga tgagccgatc
    12001 cagctgctct tcctgtcctc ggggcgcgag ggcaccgcga gcttcccgcg tgcgctggtg
    12061 gtggcgggcg aggccagcca ggtcgcgctg gtcgagtcgt tcggcagcat cggcgacggg
    12121 cgcgcgttca ccaacgcggt gaccgagctg gtggccgggc cggcggccgt cgtggaccac
    12181 taccgcctcc agcgcgagtc ggcccgcggg tatcacgtcg gcctcaccca gatccgggtg
    12241 gggcggtcgg ccaacgtctc gtcgcacaac gccgcgctcg gcgggctgat cgcccgccac
    12301 gacgcggtgg tggtgctggc agacgaggga gccgagagca cgctggacgg cgtctacctg
    12361 gccggcggac aggcgctggt cgacaaccac accgaggtcg accacgtgat gccgcacggc
    12421 accagccacg agctctacaa ggggatcctg ggagggcggg cgcgcgcggt cttcaacggg
    12481 cgcatccgcg tgcggcccga cgcgcagaag accgacgcca agcagaccaa caagacgctg
    12541 ctgctctcga acg
//
LOCUS       DAOPQX010000073        12523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5852_length_12523_cov_4.482275, whole genome shotgun sequence.
ACCESSION   DAOPQX010000073 DAOPQX010000000
VERSION     DAOPQX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5852_length_12523_cov_4.482275"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PKK95_05865"
     CDS             complement(<1..669)
                     /locus_tag="PKK95_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOC17772.1"
                     /translation="MPELPDIVVYQEALARRVAGAALEKVRVFTPFLLRTVDPPIESV
                     EGRAVEGIERLGKRIVFALAGDLFLVLHLMIAGRLHWKEKGVSPPKKVGLAAFDFAAG
                     TLTLTEAGSKRRASLHLAASREALRGLDPGGIDVLSSSLGEFEGALRSANHTLKRSLT
                     DPRLFSGIGNAYSDEILHAARLSPLQLTQNLDDAEVKRLHEATQRVLGEWIERLRAAY
                     GDGFP"
     gene            complement(693..1112)
                     /locus_tag="PKK95_05870"
     CDS             complement(693..1112)
                     /locus_tag="PKK95_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOC17773.1"
                     /translation="MPSFIVRQVFPAPPRHVFDAWVDPAVASRWLFATPDGHIVRCDI
                     DPRPGGNFVITDRRGGENVEHHGRFLEIDPPRRLAFEFSVPKYSSEVTIVRIEVAPAE
                     GGCELTLEHEGVPPEWRESTEQGWRDLLAKLEGVLRT"
     gene            complement(1121..2368)
                     /locus_tag="PKK95_05875"
     CDS             complement(1121..2368)
                     /locus_tag="PKK95_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17774.1"
                     /translation="MAVPPHDASSSHRQTVSRRRFLTGAGTAVVAYSVMRPELVRGSA
                     TNAKVNLGIIGCGGRGTWIANLFQQHGGYNLVAAADYFQDRVDAFGEKLGVPPGRRFT
                     GLSGYRRLLEQPVDAVAIITPPYFHPEQAADAVSAGKHVYLAKPVAVDTPGCKSIEQS
                     GTRATAVGRCFLVDFQTRVNPLFVEAVRRVHAGAIGRFAFGEATYHGDDPFLAQVENY
                     ESGSPEGRLRAWGLSRELSGDIITEQNIHALDVASWIMNQPPLRAYGTGGRKYRGVGT
                     CWDTFSVTFHYPDDVGVTFSSRQFNGHGTRPEGIRNRTFGSEGVLETEYGGQVLIRGK
                     QFFRGGRTPRIYEEGAVGNIAAFHDAIRRGDCANATVAESVRSNLLTILGRTAAYQGR
                     VVTWDEILGSDERLVPDLKGLVE"
     gene            2541..3719
                     /locus_tag="PKK95_05880"
     CDS             2541..3719
                     /locus_tag="PKK95_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC17775.1"
                     /translation="MTHQSAKRNRRQFLSQTSITLAAAGVAGLRSTAAAPQAASATTR
                     RRAVMRRTLGRTGLELPIVSMGVMNANNPELLRQAYDAGVRLFDTAQGYQQGRNERMI
                     GDVVSRLRVRDKVIIQTKVPFPRGSGDVKAEFLAGVAGCLERLQTDYVDILLLHAPSV
                     EQMTDAGIVSAFKELKQQKKARFVGVSHHSNQAAVLRTAAGSGLYDVALVGFNVTNAN
                     DGEFLAALKTAASRGVGLVAMKTQTGGRGRNLGVLNHPALLKWALRHTEFATAIPGFT
                     NADQLNESFSVAYGLDYSPEEKSWLADKNVQLALDFCKQCGSCLPSCPKGVDIPTLMR
                     THMYAANYANFDQARATFDEIPARASLGNCTSCAECSARCANRVVIAERISDLKAMYA
                     "
     gene            complement(3723..4673)
                     /locus_tag="PKK95_05885"
     CDS             complement(3723..4673)
                     /locus_tag="PKK95_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17776.1"
                     /translation="MKRLLLATIILSALGLASCGGNEPATKDDTTSAVPAGGRAASQE
                     LLALLPSAGEVPGWSISRGPRAFDADSLWELINGAADGFVAYGVQEVVTADYKHPGAG
                     HEAVIEIYRMKDPLNAFGKYSEERYPDYRFLDVGNEGYSGGTSVNFWTGPYYVKITTF
                     KQDDALNEELVKLARSVAGKVAAPGAEPAELSCLPKENQVPHTTKYLPKDVLAQSYFT
                     GGFEAQYKAGGKESKVVLIPMAGAAEAGEALGRYREAVGGAKGTRALRGVGEEGFAAD
                     DSFYGTIVAARRDRHVAVALGASTEDAGRKQVADVLRCAR"
     gene            complement(4787..5914)
                     /locus_tag="PKK95_05890"
     CDS             complement(4787..5914)
                     /locus_tag="PKK95_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOC17777.1"
                     /translation="MTTPKALSFVVVLALLAGCQQPRHARNVILFIGDAGGIPTLHAA
                     SIHGHRQPQGLFIQQMPHLALMDTSPADGWVTDSAAGMSAIMTGQKTDNGIISQSAAA
                     VRGEVDGEVLQTILEHAEQRGLSTGVVTNMSIADATPAACYAHANDRSEAGRIFAQLA
                     SPRFGDGPDLVVGAGRTKVLDATNAIGIDMQAALRKRGYELLDSPGGLRRGQRRVVAL
                     TDNPEFDPAPVLEYAIEALSQNHKGYFLMVEWDMHTNDVERGLNRSLTMDSLVRRAAA
                     RASEDTLIVFTADHSFDIRVRGGKREEPLVQDAVPPAAGGNGAPAAQAPQPGQRYVRM
                     DNGHTGEQVLAAAQGPGAERVRGFIKNTDLFLIIMGAYGWD"
     gene            complement(6031..6861)
                     /locus_tag="PKK95_05895"
     CDS             complement(6031..6861)
                     /locus_tag="PKK95_05895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC17778.1"
                     /translation="MALERASDEQTLAFAHLDVRKMPAHWLMARLGKRVLRPGGVETT
                     RWLLEQGEITSADEVVEFAPGLGVTATRIVAHRPAAYVGVERDETARDFVLKALRAAG
                     YGSLRARVLQADASRVPLPGGSATVVIGEAMLSMQPASKKRAIMDEAHRLLRAGGRYL
                     IHELALQPEEIDPAEAARIEHDLSAHIHVGVRIGTPAVWRRWIEDAGFEVECVSTAPM
                     RLLEPGRLLQDEGVRGTARFLFNVLRTPGAPRRMAAVRGAFRRNRRHLCAVAIVARRR
                     "
     gene            7064..8026
                     /locus_tag="PKK95_05900"
     CDS             7064..8026
                     /locus_tag="PKK95_05900"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC17779.1"
                     /translation="MHNRRLLQSLALCLAAVTASIGTAAAQPPAQAPAGAPIIGRWDL
                     TVQGPKATYPSWLEVSLSGTRTLVGRFVGGGGSARPISRVDFKDGAVHFAIPPQWEKE
                     TSDLVFDATLTGDTLEGTMVTPAGQKHRFTGRRAPSLRRSAPPTWGEQVPLFNGTDLT
                     GWTTFGGTSQWAAENGILKNAKSGANLVTTAKFTDFKLHVEVNLPKGSNSGIYLRGRY
                     EVQVEDSTNPEPKSTDLGGVYGFLVPNENAGTGAGTWQTFDITLVGRRVTVVLNGKTI
                     ICDQTIPGITGGALDSDEGAPGPIYLQGDHGAVQYRNLHITPAK"
     gene            8038..8523
                     /locus_tag="PKK95_05905"
     CDS             8038..8523
                     /locus_tag="PKK95_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17780.1"
                     /translation="MRSLVVAGGALALLLALPAEGRAQGLLRGLTDAAVTVSHDWTEA
                     DAQCGIREADIKPLAAKAAADTGLKVADDSPHAELLVTFVTLETEGTCVSHVRVALRG
                     WATTTLLYDDKKEPRAMQVVLREESTLLLSPARLHGERFRERVRGLAERIAKAVEEEN
                     R"
     gene            complement(8569..9654)
                     /locus_tag="PKK95_05910"
     CDS             complement(8569..9654)
                     /locus_tag="PKK95_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC17781.1"
                     /translation="MNKLLSIPVLVLLACSAAVLVLDAQQRQMREPGLQDALLLKDYR
                     PQSIFNTPRTDVLKAKYPAIDMHMHAPRPNTDLQAAADEILKNMDATNVQKTILFAGT
                     GKTFDQLAAVFGKHPDRFELWCGFDFSNFPASTLAGLEACVKAGGKGLGEISDKGTGY
                     RGVEPMHLDDPRMDPILEKVADLGLPMNVHMGDPQWMYEPMDASNDLLFEALYWRRDN
                     KNDDKDLAGMLQVLENAVRKHPRVTFIACHFANQTFDLDRLAKLLDKYPNLYADTSQR
                     ESYVGAIPRFARQFMEKYADRIVYGTDQGHSLPMYRHSFRVFETLDEHFYAWDVANTR
                     FALSGLGLSDATLRKLYRDNALRLLKK"
     gene            9945..10856
                     /locus_tag="PKK95_05915"
     CDS             9945..10856
                     /locus_tag="PKK95_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17782.1"
                     /translation="MRLASLAAALCALLACDQLRPPSSPSAVTPTAGEVALSWSPYVG
                     VHSNGRALRAYEDALSAMLSAGRLNGVRMSIHRQQMVDPVIKAVGSLGLEILGLVDNE
                     YLFDPDIEGAIDRIFAAYPEIRYFQIGNEVTTILPPTGPTITIEEYAAVFQRVYDHVQ
                     SRHPGRAILLTQSALGSGLRGPTELETLTRLALSRMDPDRVIVAVNSYDPDNLSQFRG
                     LLGGALRSFRVWVTESGMANPDQQVAFVRDKYPLLRRYLRAERVYWYVMWGGDEGPDT
                     NFSLIKFPTRYPDYWKSPLFTLLTEGR"
     gene            10853..11623
                     /locus_tag="PKK95_05920"
     CDS             10853..11623
                     /locus_tag="PKK95_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17783.1"
                     /translation="MTGRRVRLAGALALLCVLAPAIVSAQAAPAESVPPPQAEPAEGA
                     PPPQGRKAGTYIKVGLAHWQGDIFSESSLTRWSGDLFGADYALTSAGVEIDTYLPWGW
                     FPVSGFTIAYRKDTLSSREAGHMLSGTLFHDFDLKLLAVKVGGGVEWGVPSLNFDQTE
                     YEYLTDGTVRYRHTYPGRNAEIPVGARPNGITYPFVALSLLQRPGRLLLEVGMRVNYI
                     RFHFDDYEVRPGGETTRAFAERQVRVPYLFANVGLRLF"
     gene            11723..>12523
                     /locus_tag="PKK95_05925"
     CDS             11723..>12523
                     /locus_tag="PKK95_05925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC17784.1"
                     /translation="MPQGLASIERQDLRPLAVLVAFGVLLRVTVLLASTGILWPDTGL
                     FVGSARSLLTDQPFSLHVYKTPLYPLFLSVFMWFGETHITGQAITVTQQLMGLASTLV
                     FYLVARQVFGRTVALWSSLLFSAHTLQLFYELSVLSEALFVFLLAILIHQSMKAMRNP
                     TWDTAAFVGLLSGLATLARPVAQAFVLVILAGVILGSGWRSQLRRALAAGVVMVVAYS
                     AVVTPWMYVNFRRLGYFGVSLGHGFGLLTRVFMIDQAEPVPDTRYPDVK"
BASE COUNT         1883 a         4353 c         4292 g         1995 t
ORIGIN      
        1 gggaaagccg tcgccgtacg ctgcacggag gcgctcgatc cactccccga gcacccgctg
       61 cgtcgcctcg tgcagccgct tcacctccgc gtcgtccagg ttctgcgtca gttggagtgg
      121 ggagagccgg gcggcgtgga ggatctcgtc cgagtaggcg ttgccgatgc cgctgaacaa
      181 acgcgggtcg gtaaggctgc gcttgagcgt gtggttggcc gagcggagcg cgccctcgaa
      241 ctcgcccagc gatgacgaca gcacgtcgat gccgcccggg tcgagcccgc ggagggcctc
      301 gcgcgaggcc gcgaggtgca gcgaggctcg ccgcttgctg ccggcctcgg tgagcgtcag
      361 cgtgccggcc gcgaagtcga acgccgccag ccccaccttc ttcggaggcg agacgccctt
      421 ctccttccag tgcagccttc ccgcgatcat caggtgcagc acgaggaaca ggtcgcctgc
      481 cagcgcgaac acgatccgct tgccgaggcg ctcgatgccc tcgacggccc gaccctcgac
      541 cgactcgatc ggcgggtcca ccgtgcgaag caggaagggc gtgaacacgc gcaccttctc
      601 gagtgcggca ccggcgacgc gccgcgcgag cgcctcctgg tagacgacga tgtcgggcag
      661 ctcgggcatg gagtgctggg gtggtcgccc gctcaggtgc gcaggacgcc ttccagcttt
      721 gccaacaggt cgcgccagcc ctgctcggtg gactcgcgcc actcgggcgg cacgccctcg
      781 tgctcgagcg tcagctcgca gccgccttcg gctggcgcca cctcgatgcg gacaatcgtc
      841 acctcgctcg agtatttcgg caccgagaac tcgaacgcca ggcggcgcgg cggatcgatc
      901 tcgaggaagc gcccgtggtg ctcgacgttc tcgccgccgc ggcgatcggt gatgacgaag
      961 ttcccacccg gcctgggatc gatgtcgcag cggacgatgt ggccgtcggg cgtcgcgaac
     1021 aaccagcggc tggcgacggc gggatcgacc cacgcgtcga agacgtgccg gggcggagcc
     1081 ggaaagacct gtcgcacgat gaaggacggc atcgcggggt tcattccacg aggcctttca
     1141 agtcggggac gagccgctcg tcgctgccca ggatctcgtc ccacgtgacg acgcggccct
     1201 ggtaggccgc ggtgcgcccg aggatggtca gcaggttgct cctcacgctc tcggccacgg
     1261 tggcgttggc gcagtcgccc cgccggatgg cgtcgtggaa ggcggcaatg ttgccgaccg
     1321 ccccttcctc gtagatgcgc ggcgtccggc cgccgcggaa gaactgcttg ccgcgtatca
     1381 acacctgccc gccgtactcc gtctcgagga ccccctccga gccgaacgtg cggttgcgga
     1441 ttccctcggg cctcgtgccg tggccgttga actgccgcga gctgaaggtg acccccacgt
     1501 cgtccgggta gtggaacgtc acgctgaagg tgtcccagca ggtgccgacg ccgcggtact
     1561 tccggccgcc ggttccgtag gcccgcagcg gcggctggtt cataatccag ctggcgacgt
     1621 cgagcgcgtg gatgttctgc tcggtgatga tgtcgcccga cagctcgcgc gacagccccc
     1681 aggcgcgcag ccggccctcg ggagagccgg actcgtagtt ctcgacctgc gccaggaacg
     1741 ggtcgtcccc gtgatacgtc gcctcgccga aggcgaaccg cccgatcgcc ccggcgtgca
     1801 cgcggcgcac ggcctcgacg aagagggggt tgacccgcgt ctggaagtcc acgaggaagc
     1861 accggccgac ggcagttgcc cgggtcccgc tttgctcgat cgacttgcac cccggggtgt
     1921 cgacggctac cggcttcgcc aggtagacgt gcttcccggc cgacaccgcg tcggccgcct
     1981 gctcggggtg gaagtacggg ggcgtgatga tcgccaccgc atccaccggc tgctcgagca
     2041 gccggcggta cccggacagg cccgtgaagc ggcggccggg cgggactccc agcttctccc
     2101 cgaacgcgtc cacgcggtcc tggaagtagt cggcggcggc gacgaggttg tagccgccat
     2161 gctgctggaa caggttcgca atccaggtgc cccgcccgcc gcacccgatg atcccgaggt
     2221 tcaccttcgc gttcgtggcg ctgccccgga ccagctcggg gcgcatcacc gaatacgcca
     2281 ccaccgccgt gccggcgccc gtgaggaaac ggcgccgcga gacggtctgc ctgtgcgacg
     2341 agctcgcatc gtggggtggt actgccatgg tgacgcggat tgtagcaccg gcttcgctcg
     2401 ttgaccggcg ggccggcccg gccttaggat gcgtggcgcg ccagtcggtg agccgggcag
     2461 gtacccaggt ggacgctggg cgcggggttc gcggtacaat tccgcctcgc atctccgccc
     2521 ccgaacacaa agggcagcca atgactcacc agagcgcgaa gcggaaccga aggcaattcc
     2581 tgtcccagac gtcgatcacg ctcgccgccg cgggcgtggc cgggctgcgg agcacggcgg
     2641 cggcacccca ggcggcttcg gcgaccacca ggcgccgcgc cgtcatgcgg cgcacgctcg
     2701 ggcgaaccgg cctcgagctc ccgatcgtca gcatgggcgt gatgaacgcc aacaaccccg
     2761 agctgctcag gcaggcgtac gacgccgggg tgcggctgtt cgacacggcg cagggatatc
     2821 agcagggaag gaacgagcgg atgatcggcg acgtggtgtc gcggctccgg gtccgcgaca
     2881 aggtgatcat ccagaccaag gtgccgttcc cgcgaggctc gggcgacgtg aaggccgagt
     2941 tcctggccgg cgtcgccggc tgcctcgagc gcctgcagac cgactacgtg gacatcctgc
     3001 tgcttcacgc gccgtcggtc gagcagatga ccgacgccgg gattgtctcg gcgttcaagg
     3061 agctgaaaca gcagaagaag gcgcgcttcg tcggggtgtc gcaccactcc aaccaggcgg
     3121 ctgtcctgag gacggccgcc gggagcggcc tctacgacgt ggcgctggtg gggttcaacg
     3181 tcaccaacgc gaacgacggc gagttcctgg cggcgctgaa gaccgccgcg tcgcgcggcg
     3241 tgggcctcgt cgcgatgaag acgcagaccg ggggacgggg ccgcaacctg ggcgtcctca
     3301 accacccggc gctgctcaag tgggccctcc gccacaccga gttcgccacc gccatccccg
     3361 ggttcaccaa cgccgatcag ctgaacgaaa gcttctcggt ggcgtacggg ctcgactact
     3421 cgcctgaaga gaagtcgtgg cttgccgaca agaacgtgca gctggctctc gacttctgca
     3481 agcagtgcgg cagctgcctg ccgtcctgcc cgaagggggt ggacatcccg acgttgatgc
     3541 ggacccacat gtacgcggcc aactacgcca acttcgacca ggcgcgcgcg accttcgacg
     3601 agatccctgc ccgggcgagc ctcggcaact gcaccagctg cgccgagtgc tcggcgcgct
     3661 gcgcgaacag ggtcgtcata gcggagcgga tctccgacct gaaggcgatg tacgcctgaa
     3721 cgtcacctgg cgcagcggag gacgtcggcc acctgcttcc ggccggcgtc ttccgtggac
     3781 gccccgagcg cgacggccac gtgccggtcg cggcgggcgg cgacgatcgt gccgtagaag
     3841 ctgtcgtcgg cggcgaaccc ttcctctcca accccacgca gagcccgcgt gcccttggcg
     3901 ccccccaccg cttcgcggta gcgacccagc gcctcgcccg cctcggcggc cccggccatc
     3961 gggatcagca ccaccttcga ttccttcccg ccggccttgt actgcgcctc gaagccgccg
     4021 gtgaagtaac tctgcgcgag cacgtccttc ggcaggtact tcgtggtgtg aggcacctgg
     4081 ttctccttcg gcaggcagga gagctcggcc ggctcggccc ccggcgcggc caccttgccg
     4141 gcgaccgagc gcgcgagctt caccagttcc tcgttcaggg cgtcgtcctg cttgaaggtg
     4201 gtgatcttca cgtagtacgg ccccgtccag aagttgaccg acgtgccgcc cgagtatccc
     4261 tcgttgccga cgtcgaggaa gcggtagtcg gggtagcgct cctcggagta cttgccgaag
     4321 gcgttgagcg gatccttcat ccggtagatc tcgatcaccg cttcgtgccc ggcgcccggg
     4381 tgcttgtagt cggcggtcac cacctcctgg acgccgtagg ccacgaaccc gtctgcggcg
     4441 ccgttgatca gctcccacag gctgtcggcg tcgaaggccc gcggccccct cgaaatcgac
     4501 cagcccggca cctcgccggc cgagggcagc agggcgagca gctcctgcga cgcggccctg
     4561 cctccagcag gcaccgcgga cgtcgtgtcg tccttcgtgg ccggttcgtt tccaccgcac
     4621 gaggccaaac caagggcaga caggatgatg gtcgccagca gaagtctctt catctcgcgc
     4681 tccggaacag gggctctcga ttcgcggttg ctcgggcctc aaatcgattg ccaaggcccg
     4741 cgccatgcgc gacctcggcg acgacgagtg gccggagatg attccctcag tcccacccgt
     4801 aggcgcccat gatgatcagg aagaggtcgg tgttcttgat gaagccgcgc acgcgctcgg
     4861 cgccaggccc ctgcgccgcc gccagcacct gctcgcccgt gtggccgttg tccatgcgga
     4921 cgtagcgctg gccgggctgc ggggcctgcg ccgcgggcgc gccgttcccg ccggccgcgg
     4981 gcggcacggc gtcctgcacc agcggctctt cccgctttcc tccgcgcacc cggatgtcga
     5041 acgagtggtc ggccgtgaag acgatcagcg tgtcctcgga ggccctcgcg gcggcacgcc
     5101 gcacgagcga gtccatggtc agcgaccggt tgagccccct ctccacatcg ttggtgtgca
     5161 tgtcccattc gaccatcagg aagtagccct tgtggttctg cgagagcgct tcgatggcgt
     5221 actcgagaac cggcgccgga tcgaactcgg ggttgtcggt cagcgcaacc acgcggcgct
     5281 ggccgcggcg cagcccgccc gggctgtcca gcagctcgta ccccctcttg cgaagcgccg
     5341 cctgcatgtc gatcccgatc gcgttggtgg cgtcgagcac cttcgtccgg cccgcgccga
     5401 ccaccaggtc ggggccgtcg ccgaagcgcg ggctggcgag ctgcgcgaag atgcgtcccg
     5461 cctcgctgcg gtcgttggcg tgcgcgtagc aggcggcggg cgtggcgtcg gcgatcgaca
     5521 tgttcgtgac cacgccggtg gacaggccgc gctgctcggc gtgctcgaga atcgtctgca
     5581 gcacctcccc gtccacctcg ccgcgcaccg ccgcggccga ctgcgagatg atgccgttgt
     5641 cggtcttctg ccccgtcatg atggccgaca tgccggccgc ggagtcggtg acccagccgt
     5701 cggcgggaga ggtgtccatc aacgccaggt gcggcatctg ctggatgaac agcccctggg
     5761 gctgacggtg gccgtggatg ctcgccgcgt gcagcgtcgg gatgccgccg gcgtcgccga
     5821 tgaacaggat cacgttcctg gcgtgcctgg gctgctggca ccccgccagc agggcgagga
     5881 cgacgacgaa tgacagggct ttcggtgtgg tcacggtgtt gttcctcttc attcgaccga
     5941 cagtggctgg acgacctcct ctcagatggt aagccaggcc gcggcagggg tcgaggcggc
     6001 gggcacgagg cgacggggcc ggccgggggt ctaccggcgc cgggcgacga tcgcgaccgc
     6061 acagaggtgg cggcggttcc gccggaacgc gccgcgaacg gcagccatcc gccggggcgc
     6121 gcccggggta cggaggacat tgaacaggaa gcgcgccgtc ccgcgaactc cctcgtcctg
     6181 caggaggcgg cccggctcga gcaggcgcat cggggccgtg gagacgcact ccacttcgaa
     6241 gcccgcatcc tcgatccacc gcctccacac ggccggcgtg ccgatccgaa cgccgacgtg
     6301 gatgtgggcg gaaaggtcgt gctcgatgcg cgcggcctcg gccggatcga tctcctccgg
     6361 ttgaagggcc agctcgtgga tcaggtagcg gccgccggcg cgcagaagcc ggtgtgcttc
     6421 gtccataatc gcccgcttct tcgaggcggg ctgcatgctg agcattgcct cgccgatcac
     6481 gaccgtggcc gacccgcccg gaagcgggac gcggctcgcg tcggcctgga ggacgcgcgc
     6541 ccgaagcgag ccgtacccgg ccgcgcgaag cgccttcagc acgaagtcgc gggcggtctc
     6601 gtcgcgttcc acgccaacgt acgccgcggg acggtgcgcg acgatccgcg tggccgtcac
     6661 gccgaggccg ggggcgaact cgacgacctc gtctgccgag gtaatctccc cctgctcgag
     6721 cagccagcgc gtggtctcga ccccgccggg ccgcagcacg cgcttgccga gacgcgccat
     6781 cagccagtgc gcgggcatct tccggacgtc gaggtgggca aaggcgagcg tctgttcgtc
     6841 cgaggcacgc tcgagggcca ttgggacact tccttccagg aaagccgcgc ggcggcgggg
     6901 tacatcggtt gggacgccaa ggggaagctt atataggacc caagttgtcg tcattgatcc
     6961 agatcaacag gcggccgccc cccccacgag ccggctcatc cggcccgccc gtgcacgtcc
     7021 ctgcccttga ccttccgcgc cggcgaaggg taacgtccgg gccatgcaca atcgccgact
     7081 gcttcaatcg ctcgccctct gcctcgccgc cgtgaccgcc tcgatcggaa cggctgccgc
     7141 acagccgccg gcccaggcgc cggccggtgc gcccatcatc ggccgctggg acctgacggt
     7201 gcaggggccg aaggcgacct acccgtcctg gctcgaggtg tcgctctcgg ggacgcggac
     7261 gctcgtcggc cgcttcgtcg gcgggggcgg cagcgcccgt ccgatctcgc gcgtcgattt
     7321 caaggacgga gcggtgcact tcgccatccc gccgcagtgg gagaaggaga cgtccgatct
     7381 cgtgttcgac gccacgctca ccggcgacac gctggagggc acgatggtca cgcccgcggg
     7441 gcagaagcac cgcttcacgg ggcgccgtgc accgtcgctt cgccgcagcg ctccgccgac
     7501 gtggggtgag caggtgccgc tcttcaacgg cacggacctg acgggctgga ccaccttcgg
     7561 cgggacgagc cagtgggcgg cagagaacgg catcctcaag aacgcgaaga gcggcgccaa
     7621 cctcgtgacg accgcgaagt tcaccgactt caagctgcac gtcgaggtga acctcccgaa
     7681 ggggagcaac agcggcatct acctgcgcgg gcgctacgag gtgcaggtcg aggacagcac
     7741 gaaccccgag cccaagtcga ccgacctcgg cggggtctac ggcttcctcg tccccaacga
     7801 gaacgcggga acgggggccg gcacctggca gaccttcgac atcacgctgg tcggccgccg
     7861 cgtgacggtg gtgctcaacg gcaagacgat catctgcgac cagacgattc ccggcatcac
     7921 gggcggggca ctcgacagcg acgaaggcgc gcccggcccc atctacctgc agggcgatca
     7981 cggggccgtg cagtaccgga acctgcacat cacgccggca aagtgaagga gaccgctatg
     8041 aggagcctcg tcgtggcggg aggcgcgctg gccctgctgc tcgccctgcc ggcggagggt
     8101 cgtgcgcagg gtctcctgcg cgggttgacc gacgccgcgg tcaccgtgtc gcacgactgg
     8161 accgaggccg acgcgcagtg cgggatccgc gaggccgaca tcaagccgct ggcggcgaag
     8221 gcggcagcgg acacggggct gaaggtggca gacgactcgc ctcacgccga gctgctggtc
     8281 accttcgtca cgctcgagac cgagggcacg tgcgtctcgc acgtgcgcgt cgccctgcgc
     8341 gggtgggcca cgacgaccct gctctacgac gacaagaagg agcctcgggc gatgcaggtc
     8401 gtgctgcggg aggagtcgac acttctcctc tcgcccgcac gcctgcacgg cgagcggttc
     8461 cgcgaacgcg tgcgggggct cgccgagcga atcgccaagg cggtcgagga ggagaaccga
     8521 tagcggccgc ggctggacgg cggccgccgt ccagccgccg ctccgccact acttcttcaa
     8581 cagtctcagc gcgttgtccc ggtagagctt ccgcagggtc gcgtcgctca ggcccaggcc
     8641 ggagagcgcg aacctggtgt tggccacgtc ccacgcgtag aagtgctcgt cgagcgtctc
     8701 gaagacgcgg aacgagtggc ggtacatcgg caggctgtgg ccctggtcgg tgccgtacac
     8761 gatccggtcc gcgtacttct ccatgaactg cctggcgaac cgcgggatcg ccccgacgta
     8821 cgactcgcgc tgagaggtat cggcgtacag gttcgggtac ttgtcgagca gcttcgcgag
     8881 ccggtccagg tcgaacgtct ggttcgcgaa gtggcaggcg atgaaggtca cccgcgggtg
     8941 cttcctcacc gcgttctcca gcacctgcag catccctgcg agatccttgt cgtcgttctt
     9001 gttgtcgcgc cgccagtaga gggcctcgaa cagcaggtcg ttgctcgcgt ccatgggctc
     9061 gtacatccac tgcggatccc ccatgtggac gttcatcggc agcccgaggt cggcgacctt
     9121 ctcaaggatt gggtccatgc gggggtcgtc gaggtgcatc ggctcgacgc cgcggtagcc
     9181 ggtccccttg tcgctgatct cgccgaggcc cttgccgcct gccttcacgc acgcctccag
     9241 gccggccagc gtcgaggcgg ggaagttcga gaagtcgaac ccgcaccaca gctcgaagcg
     9301 gtcggggtgc ttgccgaaga ccgcagccag ctggtcgaag gtcttgccgg tgccggcgaa
     9361 gagaatggtc ttctggacgt tcgtcgcatc catgttcttc aggatctcgt cggcggcggc
     9421 ctgcaggtcg gtattcggcc ggggggcgtg catgtgcatg tcgatcgccg ggtacttcgc
     9481 cttcagcacg tcggtcctcg gcgtgttgaa gatcgactgc ggcctgtagt ccttcagcag
     9541 cagcgcgtcc tgcagccccg gctctctcat ctggcgctgc tgggcgtcga gaacgaggac
     9601 ggcggcggag cacgcgagca acaccagcac gggtatcgac agcagcttgt tcatgaggcg
     9661 gtctcccttg tcgcgagaag cgagggcgcg ccaattgtaa acccctcgct gcggggacgg
     9721 gtgcggtcct cggtttggct gagaccgagt gggcgttacc tgccgccggt ctggatccat
     9781 acgggcgccg ctcagctgtg aggccgcaaa acccggattc ttcgcgcgaa cagcaacatc
     9841 tggcgctggc agcttgcttg cagaggttgg ggcgtacgcg ccgcaggtgc gcccgacacc
     9901 cgatgccagc gatcactccc gggagcggtt ggcgacgagg cccgatgcgg cttgcgagcc
     9961 tggcggcggc cctctgcgcc ctgctcgcct gcgaccagct ccgtccgcca tcgtctccga
    10021 gcgccgtcac tcccaccgcc ggtgaagtcg cgctctcgtg gtcgccgtac gtgggcgtcc
    10081 acagcaacgg gcgggccctt cgcgcctacg aggatgcgct gtcggccatg ctgagcgcgg
    10141 gacggctgaa cggcgtccgc atgtcgatac accggcagca gatggtggac ccggtgatca
    10201 aggccgtggg atcgctcggc ctcgagatcc tcggcctcgt cgacaacgag tacctcttcg
    10261 atcccgacat cgagggcgcc atcgaccgca tcttcgccgc ctatccggag atccggtact
    10321 tccagatcgg gaacgaggtc acgaccatcc tgccgccgac cggccccacg atcacgatcg
    10381 aggaatatgc ggcggtattc cagcgcgtgt acgaccacgt ccagtcgcgt cacccgggcc
    10441 gggcgatcct gctcacgcag tcggccctcg ggtcgggcct gcgcgggcca accgagctgg
    10501 agacgctcac gcgcctcgca ctctcgcgga tggaccccga ccgcgtcatc gtggcggtca
    10561 actcctacga ccccgacaac ctcagtcagt tccgcggact gctcggcggc gcgctgcggt
    10621 cgttccgggt gtgggtcacc gagtcgggca tggcgaaccc cgaccagcag gttgcgttcg
    10681 tccgggacaa gtacccgctc ctgcgccgct acctccgggc cgagcgcgtc tactggtacg
    10741 tgatgtgggg cggcgacgag ggccccgaca ccaacttcag cctcatcaag ttccccaccc
    10801 gctatcccga ctactggaag agtcccctct tcaccctgct gacggaggga cgatgaccgg
    10861 gcgtcgcgtc cggctggcgg gcgccctcgc gcttctctgc gttctggcgc ccgcgatcgt
    10921 ttcggcgcag gccgcgcccg ccgagagcgt gccgccgccg caggccgagc cggccgaagg
    10981 cgccccgccc ccgcaggggc gcaaggccgg cacctacatc aaggtggggc tcgcccactg
    11041 gcagggcgac atcttcagcg agagcagcct gacgcggtgg agcggcgact tgttcggcgc
    11101 cgactacgcg ctgacgagcg caggcgtcga gatcgacacg tacttgccgt ggggctggtt
    11161 cccggtctcg ggattcacga tcgcctaccg caaggacacg ctgtcctcgc gcgaggcggg
    11221 ccacatgctg agcggcacgc tgttccacga cttcgacctg aagctgctcg cggtcaaggt
    11281 gggcggcggg gtcgagtggg gtgttccgtc gctgaacttc gaccagaccg agtacgagta
    11341 cctcactgac ggcacggtcc gctacaggca cacctacccg ggccggaacg ccgagatccc
    11401 ggtcggcgcc cggccgaacg ggatcaccta cccgttcgtg gcgttgagcc tccttcagcg
    11461 gccgggccgc ctgctgctcg aggtcggcat gcgcgtcaac tacatccgct tccacttcga
    11521 cgactacgag gtgcgcccgg gcggcgagac gacgcgcgcg tttgccgaga ggcaggtcag
    11581 ggtgccgtac ctctttgcca acgtcgggct gcggctgttc tgaggagccc gcggcctcag
    11641 cgcgccatct tgacgagcct gggctggaag cgggcgtcta cggccctggg gtccgtcaga
    11701 gtccgtggta gtatcccgcc cgatgccgca aggccttgct tccatcgaac gtcaggatct
    11761 gcgcccgctg gccgtgctgg tggccttcgg ggtgctcctc cgggtcaccg ttctgctcgc
    11821 gagtacgggc attctctggc ccgataccgg gttattcgtc gggtctgcac gatcgctcct
    11881 caccgaccag cccttctccc tccacgtcta caagacaccg ctctacccgc tgtttctttc
    11941 ggtgttcatg tggttcggcg agacgcacat caccgggcag gccataaccg tgacccaaca
    12001 gctcatgggg ctcgcctcca cgctcgtctt ctacctggtg gccaggcagg tcttcgggag
    12061 aacggtcgcc ctgtggagca gcctgctctt ctcggctcac acccttcagc tgttctacga
    12121 gctctcggtg ctctccgagg ccctgttcgt cttcctgctg gccattctca tccatcagag
    12181 catgaaggcg atgcgcaatc cgacgtggga cacggcggcc ttcgtcggcc tgctgagcgg
    12241 gctcgccacg ctcgcgcggc ccgtcgcgca ggcattcgtc ctcgtgatcc tggcaggggt
    12301 gatcctcggg agtggatggc gctcgcagct tcggagggcg ctggccgcag gcgtcgtgat
    12361 ggtggtcgcc tattcggcgg tggtcacgcc gtggatgtac gtcaatttcc ggaggcttgg
    12421 gtactttggc gtgagcctcg gccatggatt cggcctcctg acgcgcgtct tcatgatcga
    12481 ccaggcggag ccggtcccgg acacgcgcta tccagacgtg aag
//
LOCUS       DAOPQX010000074        12370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5937_length_12370_cov_4.693626, whole genome shotgun sequence.
ACCESSION   DAOPQX010000074 DAOPQX010000000
VERSION     DAOPQX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5937_length_12370_cov_4.693626"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKK95_05930"
     CDS             complement(<1..400)
                     /locus_tag="PKK95_05930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC17785.1"
                     /translation="MSILLPAHDAAATLPACLSSIGRQSLTGWECVLVDDGSTDGTGA
                     LACAAAQRDARFRVVSTPHRGLVAALQEGLRHCRAPLVARMDADDVMHRERLAAQAAL
                     LESDPTLDAAGCHVRIFPRRGMGPGLRQYEA"
     gene            complement(427..1440)
                     /locus_tag="PKK95_05935"
     CDS             complement(427..1440)
                     /locus_tag="PKK95_05935"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOC17786.1"
                     /translation="MSPKSPLYSQVEPFSRPSDRRAAVELLQTVVPFLALAAAMMFLA
                     GRGRWWIALPLALPASAFLVRTFIVFHDCCHGSFFRSRRANRWVGYFTGLLTLTPFER
                     WQRSHAEHHATVGDLDRRGSGDVWTLTVAEFRAASPFMRLYYRVFRNPFVMLGLGPAL
                     LFVIGNRWPPPDAGRRERFSVHFTNAGILATLAAAYFTVGLKVFLVTQLPTFILAATI
                     GVWLFYVQHQYEDAYWARKPEWDPMKAALEGSSYYKLPRVLQWFTGSIGLHHVHHVQP
                     RIPFYNLQRCQDTITAFQAVPPLTIRRSLHSLRLRLVDEEGRRMVTWAEVKAEAAAAG
                     RAS"
     assembly_gap    1592..1691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1692..3127)
                     /locus_tag="PKK95_05940"
     CDS             complement(<1692..3127)
                     /locus_tag="PKK95_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HOC17787.1"
                     /translation="MNASAQGLPPAHVDAFEGRPRLVVLSDIGNEPDDQMSLVRLLLY
                     SNELELEGLVATTSTWQKDKVQPGIMRRVIAAYAEARANLLLHAQGWPEASRLEGMVV
                     AGQPGYGMAAVGPGKMTAGAEAIVRAGDRADARPLWIAIWGGANTLAQALLHVRATRP
                     PAAVDAFVSKLRVRSISDQDDAGPWIRREFPHLLYIVEPSSPDSGDYLYATWTGISGD
                     DYYGNCDGADPATVTNEWLERNIRAAGPLGKAYPRFAFIMEGDTPSFLGLTDNGLNSY
                     RNPSWGGWGGRYVYRQPRAEARPIWTMGGDLFQRVTSRDLVTGRDGRPYLSDQATIWR
                     WREAFQHDFAARMGWTVRPYSEANHAPKVVVNDLAGTSPLQVDVEVGQPLTLDAAGTT
                     DPDGHELRYRWFHYPEAGFVPGARLAGLSISDANTSRATITATTACRPTWMPIKAPCP
                     SGIAHVILAVTDAGTPSLTSYRRVIVNVR"
     gene            3358..4899
                     /locus_tag="PKK95_05945"
     CDS             3358..4899
                     /locus_tag="PKK95_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOC17788.1"
                     /translation="MTQQTEPKTGPEHRRDAPLAMKADEFRRVGHQLVDTVAAFLESL
                     PGRPVTRAESPEAIRAALDASRPLPDEGKDAGALVDRAVRLLVDHSLFNGHPRFFGYV
                     TSPPAPIGILADLLAAAINPNVGAWRLAPMASEIEAQAVRWIAELAGFPAHGGGLLVS
                     GGNMANLVGFLAARAAVPSHDVRRTGLAGGPQLRVYASEETHTWIHKAADLSGLGTDA
                     IAWIRTDARQRMDVAALRAAIARDVGAGARPMLVVGTGGSVSTGAVDPLPEIAAVCRE
                     AGAWFHVDGAYGGFAALAAGAPPDLRGLALADSVALDPHKWLYAPLEAGCTLVRRPGA
                     LRDAFSYHAPYYHFGQEATNYLDLGPQNSRGFRALKVWLALQHAGRSGCLRMIEDDMR
                     LSRRLYERVAEHPELEACTQDLSITTFRFVPHDLRSRASDGEVAPYLDTLNQDVLERV
                     QNSGEAFVSNAVVGGRYLLRACIVNFRTGEADVDALPGIVARLGREADASLRAAGSFP
                     FRTRP"
     gene            4920..6419
                     /locus_tag="PKK95_05950"
     CDS             4920..6419
                     /locus_tag="PKK95_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HOC17789.1"
                     /translation="MRPDADTSAYAAAEDRFNRRRAAVGLFLGPAVFATLLALPMAGL
                     PPSAHRLSAVLGLVIVFWITEAVPLPVTALAGPVLAILLQVAPAREVFAPFADPIIFL
                     FMGSFMLAQAMYVHGLDRRVAFQALSLRWVGRSPVRVIVMFGAVSTVLSMWLSNTATA
                     AMLFPIALSIVGQASGATGGFALALMLITAFGASVGGMATPVGTPPNLIGLALLERAT
                     AIHVSFAGWMLIGVPLSALMFAFMALYFRRVCLRAGGPALAGAGLAAEELRKLGPLSR
                     GQRNVLAAFGLTVLLWIAPGLFTLAGGQEWLARAFAASVPEAVAAMIGALLLFVLPVD
                     RGARRFTLSWEDAAAIDWGIILLFGGGLSLGGMTFSTGLAEAIGRGVLAWTPAHTTLV
                     LTMVFTGLSIVLTETTSNTASATMVVPVAIAVAQAAGASPVQPALGATLGASMAFMLP
                     VSTPPNAIVYSSGRVPITAMIRHGAVIDLVGFAAVVGLVMALGDLVAGR"
     gene            complement(6480..7331)
                     /locus_tag="PKK95_05955"
     CDS             complement(6480..7331)
                     /locus_tag="PKK95_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17790.1"
                     /translation="MKASKILAVRGPGNLPSELAGIEVVHVPFSELNDAWKTADRDRS
                     REVARGWEKAAARVEGVPRETLETSAAMYLGMKSLLARREAMAITVDCLGGFYGGHIH
                     AYPCLGFHELANEGLIGACECDVRSTATMVAGTLLSKGRPGYISDPVIDTSKRQIIYA
                     HCVASNRAFGPGGPANPFEILTHSEDRQGASVRSILPAGYMTTTLELESSRKEILLHQ
                     ARAVGNDPDDRACRTKLCAEPVGDLEKLFTMWDRWGWHRVTFYGDLREPVHALADELG
                     WKVVEEA"
     gene            complement(7328..7987)
                     /locus_tag="PKK95_05960"
     CDS             complement(7328..7987)
                     /locus_tag="PKK95_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOC17791.1"
                     /translation="MSRRAFLATAGAAATGVLGLVGSPAPLEGARSPMRIRTVYALHG
                     ETQADPDWPNKGFDFRPVMSTVNGALAKACPEFEFLPTLATGEEQARKIADADKDGEV
                     AGYLVYQMNCWNRVVQALAATGKPVLYADFQFGGSGGFLVYTSALLRAHAPNVGFVAS
                     SKIDDLVAAVKCFSIVAGGGTPAAFSAAVTRARVERTPGPRAFECRDDRLDLPPSAFG
                     A"
     gene            complement(8129..9472)
                     /locus_tag="PKK95_05965"
     CDS             complement(8129..9472)
                     /locus_tag="PKK95_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOC17792.1"
                     /translation="MSLQAWNEWLNGYVWGMPMIVLLIGTGILLTVLTRAVQLRLLRR
                     ALVEVLGKLGKGGQGAGSVTPFQAVSTALASTVGVGNIAGVATAIHLGGPGALFWLWL
                     SGFFGMCTKFCEIVLALHYREPDAGGTMRGGAMFVLRKGLGLPWLGTVFAVLVPLAAF
                     GIGNMVQANSVADSLRSSFGIEPWVTGVALVLVTALVILGGITRIGEVTSLLVPFMAI
                     VYLGGGLVVLIRFAGELPHALALVFEGAFTGTAAAGGFAGSTVMMALRYGVARGLFSN
                     EAGLGSAPMVHAAAQTDHPVRQGLYGIFEVFVDTILVCTMTGLVILLTGQWSSGETGA
                     ALAGRAFSVGLPGTWGSLVVTISLVLFAYSTIIGWSYYGETGITYVLGARAARPYRLV
                     WLVFVFLGAIGSLRLVWGVADTLNGLMAVPNLVAVLGSIPLVLRLLREFEGKRHS"
     gene            9566..11473
                     /locus_tag="PKK95_05970"
     CDS             9566..11473
                     /locus_tag="PKK95_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOC17793.1"
                     /translation="MPPDLILVIVAGFCGGVVARMLRQPLVLGYLLAGVVIGPHVGVF
                     AVDDPRNLERLAEIGVTLLLFTIGLELSIRELAAVRRVALIGTPIQIALTILVGRLLG
                     SWLGLGWRESIWLGALIALSSTLVVLKTLQAQGRIGTLSSKVMLGMMMAQDLAFIPLM
                     IVLPRLSTGGLGVEEVLIALGRAGLFLALMFLVGIPLIPRLVERVARSGSRELFLLVT
                     MGLALGVGYLTHWFAVSEAVGAFVAGVVLSESDYSHQALSDIIPLRDVFSLLFFASVG
                     MLLDPMHMVTHWPQVTAVVLAVVAAKGGIFWAVTRAFGYRNVVPLASALAMFQVGEFS
                     FVLARTGLSSGAITPDLYTLVLNTAVITMVLTPLLSGLTSPIYAAMGRRRGAEPVQTM
                     NVRDTALADHVIVAGAGRVGMRIAGVLRQMGLPFVLIELDHRRIDQARERGHPIVFGD
                     AAQPEVLRGAGVQRARLLLVTTPVFEVSGQVVHRARALNPQIDVVVRAEGTAAMSALH
                     QMGVAEVVQPELEASLEMTRQALLHLRVPSLDIIHLTDRLRAEQYGPVYERHAGEYEL
                     LARVGAAARQLDLRWVRVEEGSRLAGASIGGLAVRPSMGVTIVGVVGAAGFTNGPGPE
                     HVFQPGASVER"
     gene            11480..>12370
                     /locus_tag="PKK95_05975"
     CDS             11480..>12370
                     /locus_tag="PKK95_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOC17794.1"
                     /translation="MPMYADVTVPTAFLVIAILAILVQSLLLFLALFGPGLRYRTRHT
                     ELQDMDPAEFLRTLAAVTDAHPNYHTELDVFTNGEQFYEAELAEIARARRTINIEAYI
                     FRPGEVARQFVAALAERARAGVEVRIVLDGIGSFRTTARDFAPVLTAGGKLSFYHPLR
                     LTTIPRYNNRTHRELVIVDGETAFVGGAGFADNWLLSRPGHPRWRDTMIRVRGRAVTS
                     LQGTFVENWLEASGEMIAGDAHFPDCDPAGGSPTIVVNSTPSAGGSTRARMLFQMLLA
                     SARESIHLTTPYFLPDASLAD"
BASE COUNT         1781 a         4346 c         4323 g         1820 t
ORIGIN      
        1 acgcctcgta ctgccggagg cccgggccca tcccgcgccg gggaaagatc cgcacgtggc
       61 agccggccgc gtcgagcgtg gggtcgctct cgagcaaggc agcctgcgcc gccagccgct
      121 cgcggtgcat cacgtcgtcg gcatccatgc gcgcgacgag cggggcccgg cagtggcgca
      181 gcccttcctg gagggccgcg acgaggccgc ggtgcggcgt cgacacgacg cggaagcgcg
      241 cgtcccgctg cgcggcggcg caggcgagcg cgccggtgcc atcggtcgag ccgtcgtcca
      301 cgagcacgca ctcccaaccg gtgagcgact ggcggccgat gctggagagg caggccggca
      361 gcgtggcggc ggcgtcgtgc gccgggagca ggatcgacac gcgcggcgcc gcggccgccg
      421 cgccactcac gaggcccggc ccgcggccgc cgcctcggcc ttcacctcgg cccaggtgac
      481 catccgccgg ccctcctcgt cgaccaggcg caggcgcagc gagtgaaggc tgcgccggat
      541 ggtgagcggc ggaaccgcct ggaacgccgt gatcgtgtcc tggcaccgct gcaggttgta
      601 gaagggaatg cgcggctgca cgtgatggac gtggtgcagc ccgatgctgc cggtgaacca
      661 ctggagcacg cgaggcagct tgtagtacga gctgccctcg agcgccgcct tcatcggatc
      721 ccactccggc ttccgcgccc agtaggcgtc ctcgtactgg tgctgcacgt agaacagcca
      781 gaccccgatg gtggcggcga ggatgaacgt gggcaactgc gtcaccagga acaccttcag
      841 cccgacggtg aagtaggccg ccgccagcgt cgcgaggatg ccggcgttgg tgaaatggac
      901 actgaaccgc tcgcgccggc cggcatcggg cggcggccac cggttgccga tcacgaagag
      961 cagcgccggc ccgagaccga gcatcacgaa ggggttgcgg aacacgcggt agtacaggcg
     1021 catgaacggc gacgccgccc ggaactcggc gaccgtcagc gtccagacgt cgccactccc
     1081 gcggcgatcg aggtcgccga ccgtcgcgtg gtgctcggcg tgcgaacgct gccaccgctc
     1141 gaacggcgtc agcgtcagca ggccggtgaa gtacccgacc cagcggttgg cgcggcgcga
     1201 gcggaaaaac gacccgtgac agcagtcgtg aaagacgatg aaggtccgga cgaggaacgc
     1261 cgatgccggc agggcgagcg gcagggcaat ccaccagcgc ccgcggcccg cgaggaacat
     1321 catcgcggcg gcgagggcga ggaacggcac gacggtctgc agcagctcga cggcggcgcg
     1381 gcgatcgctc ggacggctga acggctcgac ctgcgagtag agaggcgact tgggggacac
     1441 gggatccagt gtaacggaag gcggagggag ggagggcgca gaacctcggg ctcaggcttc
     1501 aaggctcacg gctcaagggc ctgccgcgcc gcgggccgca cgttgacgat gacgcggcga
     1561 tacgaggtca gcgagggcgt gccagcgtcg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn ncgcacgttg acgatgacgc ggcgatacga ggtcagcgag ggcgtgccag
     1741 cgtcggtcac ggccaggatc acgtgggcga tgccagatgg gcacggcgcc ttgattggca
     1801 tccaggtggg gcggcaggcc gtcgtggccg tgatcgtcgc gcgagaggtg ttggcgtccg
     1861 agatggacag gcctgccagc cgcgcgcccg ggacgaaacc ggcctcgggg tagtggaacc
     1921 agcgatagcg cagctcgtga ccgtcgggat cggtcgtccc cgcggcgtcg agcgtgagcg
     1981 gctgccccac ctcgacgtcg acctgcagcg gcgaggttcc cgccaaatca ttcacgacaa
     2041 ccttcggcgc gtggttcgct tccgaataag gcctgacggt ccagcccatc cgcgcggcga
     2101 agtcgtgctg gaacgcctcg cgccagcgcc agatcgtggc ctggtccgac aggtacgggc
     2161 ggccgtcgcg gccggtgacg aggtcgcgcg acgtcacgcg ctggaagaga tcgccgccca
     2221 tcgtccagat tgggcgagcc tcggccctgg gctgccggta gacgtagcgc ccgccccagc
     2281 cgccccagct cgggttgcga tagctgttca ggccgttgtc ggtcaggccg aggaacgacg
     2341 gcgtgtcgcc ctccatgatg aacgcgaacc gcgggtacgc cttccccagc gggccggcgg
     2401 ccctgatgtt ccgctcgagc cactcgttgg tcacggtggc cgggtcggcg ccgtcgcagt
     2461 tcccgtaata gtcgtcgccg ctgatgccgg tccaggttgc atagaggtag tcgccgctgt
     2521 cgggactgct gggctcgacg atgtacagca ggtgagggaa ctcgcggcgg atccacggcc
     2581 ccgcgtcgtc ctggtcggag atcgagcgga cgcgcagctt cgagacgaag gcgtccacgg
     2641 cggccggagg gcgtgtggca cgcacgtgca gcagcgcctg ggccagggta ttggcgccgc
     2701 cccagatcgc gatccacaac ggccgggcgt cggcgcgatc gccggcccgc acgatcgcct
     2761 cggcgccggc cgtcatcttg ccgggcccga cggcagccat gccgtagcct ggctgtcccg
     2821 cgacgaccat cccctcgagc ctggaggcct cgggccaccc ctgtgcgtgc aggagcaggt
     2881 tcgcacgcgc ctcggcgtac gcggcaatca cccgccgcat gatgccgggc tgcaccttgt
     2941 ccttctgcca ggtcgaggtg gtggcgacca ggccctcgag ctccagctcg ttcgagtaga
     3001 gcagcagcct gaccaatgac atctggtcgt ccggctcgtt gccgatgtcg ctgaggacga
     3061 cgaggcgcgg gcggccctcg aacgcgtcca cgtgggccgg gggaagcccc tgcgcggacg
     3121 cgttcacgga tgctgcgaga agaagggcga cagcgtgcgc acgaaccacc atggtgaccc
     3181 ccgtcgcgcc aacgccgcgg cgcaacggga gtatagacgc cggcggaacg atcaagaacc
     3241 acaaaggacg ccccctcacc cggccgtccc gagggacggc cgccgaagac cgtccgtcaa
     3301 cgagcctctg ggatctttgt gatctttgtg atctttgtgg tttgatccct tcaaggcatg
     3361 acccaacaaa ctgaaccgaa gacagggccc gagcaccggc gagacgcgcc gctcgcgatg
     3421 aaggccgacg agttccgccg cgtaggccac cagctggtgg atacggtcgc cgcgttcctg
     3481 gagtcgctgc ccgggcggcc ggttacgcgc gccgaatcgc cagaggcgat ccgggccgcg
     3541 ctcgacgcct cgcgcccgct gcccgacgag ggcaaggatg ccggcgccct ggtcgaccgg
     3601 gcggtgcgcc tgctcgtcga tcactccctg ttcaacgggc acccgcgctt cttcgggtac
     3661 gtcacgtcgc cacccgcgcc gattggcatc ctggccgatc tgctcgccgc cgcgatcaac
     3721 ccgaacgtcg gcgcctggcg gctggcgccg atggccagcg agatcgaggc gcaggcggtg
     3781 cgttggattg cggagctggc ggggttcccc gcgcacggcg gcggcctgct cgtcagcggc
     3841 gggaacatgg cgaacctggt cggcttcctc gcggcgagag ccgccgtccc gtcgcacgat
     3901 gtgcggcgca ccggcctcgc gggaggccca cagcttcgcg tttacgcctc cgaggaaacg
     3961 cacacctgga tccacaaggc agccgacctg tcggggctgg gaaccgacgc catcgcctgg
     4021 atccgcacgg atgcccgcca gcggatggac gtcgccgcgc tccgggcggc gatcgcgcga
     4081 gacgtcgggg ctggcgcccg cccgatgctg gtcgtgggga ccggcggctc ggtgagcaca
     4141 ggcgccgtcg atccgctccc cgagattgcc gccgtgtgca gggaagccgg cgcgtggttc
     4201 cacgtggacg gcgcctacgg cggcttcgcg gcgctggccg ctggcgcccc gccagatctc
     4261 cgggggctcg cgctcgccga cagcgtggcg ctcgacccgc acaagtggct gtacgccccg
     4321 ctcgaggccg gctgcacgct ggtgcgccgc cccggcgcgc tgcgcgacgc cttctcgtac
     4381 cacgcgccct actaccactt cggccaggag gcgaccaact acctcgacct cgggccccag
     4441 aactcgaggg gcttcagggc cctcaaggtg tggctcgccc tccagcacgc ggggcgttcc
     4501 gggtgcctga ggatgatcga ggacgacatg cgtctcagcc ggcgcctcta cgagcgggtc
     4561 gccgaacacc cagagctcga ggcctgcacg caggacctga gcatcacgac gttccgtttc
     4621 gtcccgcacg acctgcgcag ccgggccagc gatggcgagg tggcgccgta cctcgacacg
     4681 ctcaaccagg acgtgctgga gcgggtgcag aactcggggg aagcgttcgt gtcgaacgcg
     4741 gtggtggggg gccgctacct gctgcgcgcc tgcatcgtga acttccgcac cggggaggct
     4801 gacgtggacg cgctgccggg gatcgtggcg cggcttggcc gcgaggccga cgcctcgctg
     4861 cgggcggccg gatcattccc cttccgaaca cggccgtaag ccattactgt atcgggcgca
     4921 tgaggcccga cgccgacacc tccgcctacg cggccgcgga agaccggttc aaccggcgcc
     4981 gggccgcggt tggcctcttc ctgggcccgg cggtcttcgc cacgctgctc gcgctcccga
     5041 tggcgggcct gccgccctcg gcgcaccggc tgtccgcggt tctcggcctg gtgatcgtct
     5101 tctggatcac cgaggccgtc ccgctgccgg tgacggccct cgccggcccg gtgctcgcca
     5161 tcctgctcca ggtggcgccg gcccgcgagg tgttcgcccc cttcgccgac ccgatcatct
     5221 tcctcttcat gggcagcttc atgctggccc aggcgatgta cgtgcacgga ctcgaccgcc
     5281 gcgtggcctt ccaggcgctg tcgctccgct gggtgggccg gagccccgtg cgggtgatcg
     5341 tgatgttcgg cgccgtttcg accgtcctgt cgatgtggct cagcaacacc gcgaccgcgg
     5401 ccatgctgtt ccccatcgcc ctgtcgatcg tcggccaggc gagtggggcc accggaggct
     5461 tcgcgctcgc gctgatgctg atcacggcct tcggcgcatc ggtggggggc atggccacgc
     5521 cggtcggcac gccgccaaat ctcatcggcc tcgcgctgct ggaacgcgcc accgccatcc
     5581 acgtctcgtt cgccgggtgg atgctgatcg gcgtcccgct gtcggccctg atgttcgcct
     5641 tcatggcgct gtacttccgg cgggtctgcc tgcgggccgg tgggccggcg ctcgcggggg
     5701 ccggcctggc cgcggaggaa ctgcggaagc tcgggccgct ctcgcgggga cagcgtaacg
     5761 tgctcgccgc gtttggcctg acggtgctgc tgtggatcgc gcccgggctc ttcactctcg
     5821 cgggcgggca ggaatggctg gcgcgcgcct tcgccgcctc ggtcccggaa gcggtggccg
     5881 cgatgatcgg cgccctgctg ctgttcgttc tgccggtcga tcgcggggcg cggcgcttca
     5941 cgctctcgtg ggaggacgcg gccgcgatcg actgggggat catcctgctg ttcgggggcg
     6001 gcctctcgct cgggggaatg accttctcga ccggactggc cgaggcgatc gggcggggcg
     6061 tcctcgcctg gacgccggcg cacaccacgc tggtcctcac catggtgttc accggcctct
     6121 cgatcgtgct caccgagacg acgtcgaaca cggcgtcggc gacgatggtg gtgcccgtgg
     6181 ccatcgcggt ggcgcaggcg gccggggcct cgccggtgca gcccgcgctc ggcgccaccc
     6241 tcggcgcgag catggcgttc atgctccccg tctcgacgcc gcccaacgcg atcgtctaca
     6301 gctctgggcg cgtgcccatc accgcgatga tccggcacgg cgcggtgatc gacctcgtgg
     6361 gcttcgccgc cgtcgtcggg ctggtgatgg cgctcggcga tctcgtcgcg gggcggtgat
     6421 ccaccggcga gacgccggtg ccactgtgcc tcaccggcga gacgccggtg ccactgtgcc
     6481 tacgcttcct ccaccacttt ccatcccagc tcgtcggcca gcgcgtgtac cggctccctg
     6541 agatcgccgt agaaggtgac ccggtgccag ccccaccggt cccacatcgt gaagagcttc
     6601 tcaaggtcac cgaccggctc ggcgcacagc ttcgtgcggc acgcgcggtc gtcgggatcg
     6661 ttgcccaccg ccctggcctg gtggagcagg atctccttgc ggctcgactc gagttcgagc
     6721 gtcgtcgtca tgtagccggc gggaaggatc gagcgcaccg aggcgccctg ccggtcttcg
     6781 gagtgcgtga gaatctcgaa ggggtttgcc gggccgccgg ggccgaacgc ccggttcgag
     6841 gcgacgcagt gggcgtagat gatctgcctc ttcgacgtgt cgatcaccgg gtcggagatg
     6901 tagcccgggc ggcccttgct gagcagcgtg cccgcgacca tcgtggcggt ggagcgcacg
     6961 tcgcattcgc aggccccgat cagcccttcg ttcgccagct cgtggaagcc caggcacggg
     7021 taggcgtgga tgtgtccgcc gtagaagccc ccgaggcagt ccacggtgat ggccatcgcc
     7081 tcgcgcctgg cgagcaggct cttcatccca aggtacatcg ccgccgaggt ttcgagcgtc
     7141 tctcgcggca ccccttccac ccgagcggcg gctttctccc accctctcgc cacctcgcgc
     7201 gacctgtcgc ggtcggccgt cttccaggca tcgttcaact cggagaaggg gacgtgcacg
     7261 acctcgatcc cagccagctc ggaggggagg ttgcctggcc cgcgcaccgc caggatcttc
     7321 gacgccttca tgcgccgaac gcactcggcg gcaggtcgag ccggtcgtcc cggcactcga
     7381 acgcccgcgg ccccggggtc cgctccacgc gcgcacgcgt caccgcggcc gagaacgctg
     7441 ccggcgtccc gccgcctgcg acaatcgaga aacacttcac cgccgccacc aggtcgtcga
     7501 tcttcgaaga ggcgacgaac ccgacgttcg gggcgtgggc gcgcagcagc gcggaggtgt
     7561 agacgaggaa cccgccgctg cccccgaact ggaagtcggc atagagaacc ggcttcccgg
     7621 tggcggcgag cgcctggacg acgcggttcc agcagttcat ctggtagacg aggtagccgg
     7681 caacctcgcc gtccttgtcc gcatcggcaa tcttccgggc ctgctcctcg ccggtggcca
     7741 gcgtcggcag gaactcgaac tcggggcacg ccttcgcgag cgcgccgttg acagtcgaca
     7801 tgaccggccg gaagtcgaag cccttgttcg gccagtcggg gtcggcctgg gtctcgccgt
     7861 gcagggcgta gacggtgcgg atgcgcatcg gtgaccgggc cccctcgaga ggtgccgggg
     7921 aaccaacgag gccgagcacg ccggtcgcgg cggcgccagc ggtggccagg aaggcccggc
     7981 ggctcaccgc agccggacac ccgtggcacg aaggaggaac gcagcggcgg ccttgttcga
     8041 acttctctgg catacgggca ccctccctgc gacgggccgg ccaacgggcg gccacaagcc
     8101 ggtcaaaggg cggccacaag ggccgcccct acgagtgtct cttcccctcg aattccctca
     8161 gcagccgcag cacgagcggg atggacccca acacggcgac gaggttcggg acggccatca
     8221 ggccgttcag cgtgtcggcg acgccccaga cgaggcgcag ggaaccgatc gcgccgagga
     8281 acacgaacac cagccacacg agccggtacg ggcgggcggc ccgcgcaccg agcacgtagg
     8341 tgatccccgt ctcgccgtag tagctccacc cgatgatcgt ggagtaggcg aacagcacga
     8401 gcgagattgt cacgaccagg ctgccccagg tgcccggcag gcccacgctg aaagcgcgac
     8461 cggccagcgc cgcccccgtc tcgccgctcg accactgccc cgtcagcagg atgaccaggc
     8521 ccgtcatcgt gcacacgagg atcgtgtcca cgaacacctc gaagatcccg tagagcccct
     8581 ggcgcaccgg gtggtcggtc tgcgccgccg cgtgcaccat cggcgcgctg ccaaggcccg
     8641 cctcgttcga gaagagcccg cgcgcaacgc cgtagcgcag cgccatcatc acggtgctgc
     8701 ccgcaaagcc gccggccgcc gcggtgccgg tgaacgcccc ctcgaacacg agcgcgagcg
     8761 cgtgcggcaa ctcgccggcg aagcggatca gcacgacgag gccgccgccc aggtacacga
     8821 tggccatgaa ggggacgagc agcgaggtca cctcgccgat gcgcgtgatg ccgccgagga
     8881 tgacgagcgc cgtcaccaac accagcgcca cccccgtcac ccacggctcg atgccgaagc
     8941 tcgacctgag cgagtcggcg accgagttgg cctgcaccat gttgccgatg ccgaaggccg
     9001 ccagcggcac gagcacggcg aagacggtcc ccagccacgg caggccgagg cccttcctga
     9061 gcacgaacat cgcgccgccc ctcatcgtcc cgcccgcgtc gggctcgcgg tagtgaaggg
     9121 cgagcacgat ctcgcagaac ttcgtgcaca tcccgaagaa ccccgacagc cagagccaga
     9181 agagggctcc cgggccgccc aggtggatcg cggtggcgac acctgcgatg ttgcccacgc
     9241 ccacggtcga ggccagcgcg gtggagacag cctggaacgg tgtgacgctt cccgccccct
     9301 ggcccccctt ccccaacttg ccgagcacct cgacgagcgc gcggcgcagc aggcgcagct
     9361 gcacggcgcg ggtcagtacg gttagcagga tgcccgtgcc gatcagcagc acgatcatcg
     9421 gcatgcccca cacgtaccca ttcagccatt cgttccaggc ttgcaggctc atgtcggcgg
     9481 agtatagacc aaaggctcag ggctcagggc cctgagcctt gagtcctgag cgtttgaacc
     9541 ctcagcccgt ttagaatggg gcccggtgcc gcccgatctg atcctcgtca tcgtcgccgg
     9601 gttctgcggg ggagtcgtcg cgcgcatgct gcgccagccc ctcgtgctcg gctacctgct
     9661 cgccggcgtg gtgatcgggc cgcacgtcgg cgtcttcgcg gtcgacgacc cacgcaacct
     9721 cgagcggctg gccgagattg gcgtcacgct gctgctgttc acgatcgggc tggagctctc
     9781 gatccgcgag ctggcggccg tgcggcgggt ggcgctcatt gggacgccca tccagatcgc
     9841 cctcaccatc ctcgtcggcc gcctcctcgg gtcgtggctc ggcctgggct ggcgcgagtc
     9901 gatctggctg ggcgccctga tcgccctctc cagcacgctg gtggtcctca agacgctcca
     9961 ggcgcagggg cggatcggga cgctgtcgag caaggtgatg ctcggcatga tgatggcgca
    10021 ggacctggcg ttcatcccgc tgatgatcgt gcttccccgc ctgagcaccg gcgggctggg
    10081 cgtcgaggaa gtgctcatcg cgctggggcg cgccgggctg ttcctggcgc tgatgttcct
    10141 ggtcggcatc ccgctcatcc cgcggctcgt cgagcgcgtc gcccggtcgg gctcgcgcga
    10201 gctgttcctg ctcgtcacga tggggctcgc gctcggcgtg ggctacctga cgcactggtt
    10261 cgccgtctcc gaggctgtcg gtgcgttcgt cgcgggcgtg gtgctgagcg agtccgatta
    10321 cagccaccag gcgctgagcg acatcatccc gctgcgcgac gtgttcagcc tgctgttctt
    10381 cgcctcggtc ggcatgctgc tcgacccgat gcacatggtc actcactggc cccaggtgac
    10441 ggcggtggtc ctggcggtgg tcgcggcgaa gggcggaatc ttctgggcgg tcacccgggc
    10501 gttcggctac cgcaacgtcg tgccgctggc cagcgcgctc gcgatgttcc aggtgggcga
    10561 gttctccttc gtgctggcgc ggaccggcct ctcctctggc gcgatcacgc ccgatctcta
    10621 caccctggtt ctcaacacgg ccgtgatcac catggtgctc acgccgctgc tctcggggct
    10681 gacctcaccg atctacgccg cgatgggccg gcgcaggggc gccgagccgg tgcagacgat
    10741 gaacgtgcgc gacacggcgc tggccgatca cgtcatcgtg gccggtgcgg gccgcgtggg
    10801 gatgcgcatc gccggcgtgc tcaggcagat gggccttccc ttcgtcctga tcgagctgga
    10861 ccaccgccgc atcgaccagg cacgcgagcg cggccacccg atcgtgttcg gcgacgcggc
    10921 acagcccgag gtgcttcgcg gcgccggcgt ccagcgcgcg cggctgctgc ttgtgacgac
    10981 gccggttttc gaggtgagcg gccaggtggt gcaccgggcc cgggcgctca acccgcaaat
    11041 cgacgtggtc gtgcgggccg aggggacggc ggccatgtcg gcgctgcacc agatgggtgt
    11101 cgccgaggtg gtgcagcccg agctcgaggc gagcctcgag atgacgcgcc aggcgctgct
    11161 gcacctgagg gtgccgtcgc tcgacatcat ccacctgacc gaccgcctgc gcgccgagca
    11221 gtacggaccc gtgtacgagc ggcacgccgg cgagtacgag cttctggccc gggtgggggc
    11281 cgccgcccga cagctcgacc tccggtgggt gcgcgtggag gagggcagcc gccttgccgg
    11341 ggcctcgatc ggcgggctgg ccgtccgccc ttcgatgggg gttacgatcg tcggcgtggt
    11401 gggcgccgcc ggtttcacga acgggccggg cccggagcac gtctttcagc cgggcgcttc
    11461 cgtggagcgc tgagccgcga tgccgatgta cgccgacgtg accgtgccga ccgccttcct
    11521 ggtcatcgcg atcctggcga tcctcgtgca gtcgctgctg ctgttcctgg cgctgttcgg
    11581 gccgggcctc cgctaccgca cccgccacac ggagctgcag gacatggacc ccgccgagtt
    11641 cctgcggacc ctggccgcgg tgaccgacgc ccacccgaac taccacaccg agctcgacgt
    11701 cttcaccaac ggcgagcagt tctacgaagc cgagctggcc gaaattgcgc gggcaaggcg
    11761 gacgatcaac atcgaggcct acatcttccg gccgggcgag gtggcccgcc aattcgtggc
    11821 cgcgctcgcc gagcgcgccc gcgccggcgt ggaggtgcgc atcgtcctcg acggcatcgg
    11881 gtcgttccgg acgacagcca gagacttcgc ccccgtgctg acggccggag ggaagctgtc
    11941 cttctaccac ccgctgcgcc tgacgacgat cccgcgctac aacaaccgga cgcaccgcga
    12001 gctcgtcatc gttgacggcg agacggcttt cgtgggcggc gcgggctttg ccgacaactg
    12061 gctgctgtcg aggcccgggc acccccgctg gcgcgacacg atgatcaggg tgcgcggccg
    12121 cgcggtgacc agcctgcagg gaaccttcgt cgaaaactgg ctcgaggcga gcggcgagat
    12181 gatcgccggc gatgcccact tccccgactg cgaccccgcg ggagggagcc ccaccatcgt
    12241 ggtgaacagc acgccgtcgg ccggcggatc cacacgcgcc cgcatgctgt tccagatgct
    12301 gctcgcctcg gcgcgggaga gcatccacct caccacgccg tacttcctgc ccgatgccag
    12361 cctcgccgac
//
LOCUS       DAOPQX010000075        12244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_5998_length_12244_cov_5.234228, whole genome shotgun sequence.
ACCESSION   DAOPQX010000075 DAOPQX010000000
VERSION     DAOPQX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5998_length_12244_cov_5.234228"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKK95_05980"
     CDS             <1..835
                     /locus_tag="PKK95_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOC17795.1"
                     /translation="LHLHTTASDGRCPPREIAAVARRAGLGVIAVTDHDTVAGLREAA
                     EGTRSQGIRLVSGIEITAVQEDADVHVLGYFFDPEARDLGLFLASQRGDRLRRLRAMA
                     AKLAALGMPLDVDRILDAAGGASSGRAVGRPMLARALVDAGYLESRRLAFDLYLAEGQ
                     PAWVPRRAPSPAEVVEVIHQAGGLASLAHPGLLGRDEWIPPLASSGLDALEVYYSDHS
                     PELTIHYRELASRLKLGMTGGSDYHGDPAHGSERPGGCVLPPEAFEGLEALRSHRDGT
                     Q"
     gene            832..1131
                     /locus_tag="PKK95_05985"
     CDS             832..1131
                     /locus_tag="PKK95_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HOC17796.1"
                     /translation="MTITFAPEAREAVRAWLARDPSKPALRLFFAGGCGALGYQLTPA
                     ALEPRPGEQAIEVDGVTLYADFKSSTDLDGARIELGESPDDIVLVHDSCVVGGMC"
     gene            1168..1875
                     /locus_tag="PKK95_05990"
     CDS             1168..1875
                     /locus_tag="PKK95_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC17797.1"
                     /translation="MNVLELRGIVKAHGGLRPFRLRRLAVARGEVVVIEGPDAAQAAV
                     LVDLLTGTTLPDQGEVLVDGRNTRTIESQDEWLAFLERFGLSGERVVLLPELTLVQNL
                     AIPLTLSVEPIPTSIRPRVDALAEEAGLPAGRLETAVARAGPAERARVRLGRAIAHDP
                     AVLLLEHPTAGMSEAEASSFGADLRAMASRRGLTVVAASADRRFSGAAATRALAWTAA
                     TGELIRSGGSLRRLFGF"
     gene            2052..2255
                     /locus_tag="PKK95_05995"
     CDS             2052..2255
                     /locus_tag="PKK95_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOC17798.1"
                     /translation="MPTGTIKRLVRDRGFGFIRDDGGQEWFFHRSAVAQGAFDQLNEG
                     QRVDFDEEPSNKGPRANNIRIEA"
     gene            complement(2684..2941)
                     /locus_tag="PKK95_06000"
     CDS             complement(2684..2941)
                     /locus_tag="PKK95_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17799.1"
                     /translation="MSERSGSTRSAPASSNTSSLNPPVATPIGRAPWACPHATSSAVS
                     PTTTASAAEAALSPTAAARSSATGTRSFLSAASSPNAPNRK"
     gene            complement(2980..3075)
                     /locus_tag="PKK95_06005"
     tRNA            complement(2980..3075)
                     /locus_tag="PKK95_06005"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3039..3041),aa:Sec,seq:tca)
     gene            complement(3312..3518)
                     /locus_tag="PKK95_06010"
     CDS             complement(3312..3518)
                     /locus_tag="PKK95_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOC17800.1"
                     /translation="MPTGTIARLLIDKGFGFIRDEGGIEHFFHRSSVRGAVFELLREG
                     QRVEFTPEESAKGPRAGEVRLIEG"
     gene            complement(3815..4006)
                     /locus_tag="PKK95_06015"
     CDS             complement(3815..4006)
                     /locus_tag="PKK95_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17801.1"
                     /translation="MAEQLRINCQRCGTPMEMKDPAPGGPWKPDQYWVCPRCGRHFWS
                     TYPNPPGAAPDKAPGGPKT"
     gene            4032..4640
                     /locus_tag="PKK95_06020"
     CDS             4032..4640
                     /locus_tag="PKK95_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOC17802.1"
                     /translation="MVGGIILAAGASSRMGRAKALLPSGDGRTFLECLASGFSEAGLD
                     PLLVVAGSHADEIAALVRAKSLPLHLVVNPRPERGQLSSLVAGLDALAGRAIDAVLVT
                     PVDHPLVSPATVRRVVDAWRESGALVVRPERSGRHGHPVVFAAALFDELRHADLSVGA
                     RAVIARHSAEVLDVPVGEPGAFEDIDTPDDYRRLVGQALPPR"
     gene            complement(4685..6130)
                     /locus_tag="PKK95_06025"
     CDS             complement(4685..6130)
                     /locus_tag="PKK95_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HOC17803.1"
                     /translation="MSIAPVPGSFPRPLDSYPPPPDPSSLLSTLASRVETDPFNAVAT
                     AIFLLAIVHTFAAGRFARLSHQVQHRHDERARARGERPRPSVLAELLHFVGEVEVVFG
                     LWAVVLFAAIALSMGWETAKGYFNGTVNYTEPLFVVVIMALASTRPVVVFAEGALRRL
                     ADAGGGTPAAWWVSILTIGPLLGSFITEPAAMTICALLLGRQFYDREPSAALKYATLG
                     LLFVNVSIGGTLTHFAAPPVLMVARPFSWDTPYMLLHFGWRAALAIAVSTLACYLLFR
                     KELSRLASSPSPDEVEQPDDAAAPGEALLPVPAWVTVVHLGFIGWTVLTAHYPALFIG
                     GFLFFVGFALATPMYQQRLELRTPLLVGFFLGGLVIHGGLQGWWIAPVLGSLTAFPLF
                     AGSMVLTAFNDNALITYLATLVPNLGEELRLAVVQGAVVGGGLTVIANAPNPAGQALL
                     RRFFGGAVSPLGLLAGAIGPTLLTAAIFRLF"
     gene            complement(6189..8585)
                     /locus_tag="PKK95_06030"
     CDS             complement(6189..8585)
                     /locus_tag="PKK95_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOC17804.1"
                     /translation="MRCVAAFVSFATLASSFAASSSAQTTDRIYFPAVQNVRSVLVAA
                     IRAETVRIDMAAWLLTERMVSTALRDAFRRGVQVRLIGDRVALFETDPLTRNEFYWLA
                     SQGVPIRVRTHPRSFPEIAHWKAAIFKGQRLVSFGSANYTPNQLAPASEDNYSDETVL
                     FTTDPSLVGAFLTKFDAMWNDTTPEPESRGTAPPYLLNWDEACGLESACSSYRSLYPA
                     PAPMLVNTARLEPDRPMPAGLVWGQGASFNNRLAREIANEERLVRFVVYRLTSETVTR
                     ALVEKARSGVPVRLIVEPAEYLNRRWPEFWLTHANLDRLWAAGVRIRQRRHAGLTHMK
                     MLVTSRYATNASSNVATNWQRDHNYFVPAATKPAAHQAMAARFDQMWNDAEAFEPFVP
                     GPPDQPRLAAPQAGASGVVEATALSWRAAPFATSYDVYLGPSPAALSPVARVAAQMVN
                     DPPATYSWAPPAALSPGQTLFWKVVARTNATGRDPSLVRDSGAWSFTTLGAAADARRS
                     RPTVFRRSNGRWYIQGRADSIRFGAASDVPVHADYDGDGVLDAAVWRPSSGTWFILRS
                     AGSPQMQVQWGSGNSRDVPLPADYDGDGRADLAVWRPATGTWFILRSSGGYQRSSALV
                     VRWGAATDQPVPADYDGDGRADLAVWRPGTGTWWILTSTSGFRTEDRLAVAWGSGRLL
                     DVPVPADYDGDGRADLAVWRPGTGTWWILQSADGYSADAQVHVRWGLGSLDDVPVPAD
                     YDGDGRADLAVWRPGPGTWYFLWSATGYSTWSSTQWGVRSAGDVPVLAGWAAMQSYLR
                     "
     gene            8871..9758
                     /locus_tag="PKK95_06035"
     CDS             8871..9758
                     /locus_tag="PKK95_06035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOC17805.1"
                     /translation="MPRKTAHDFDQELLILFDAYVHGLIDRRAFLDKAGKFAVGGMTA
                     AMLLDQLSPKFLEAQVIKPDDPRIKAQHVEYESPKGSGKMRGYLAQPAKPAGRLPGVL
                     VIHENRGLNPHIEDVARRLALENFVAFAPDALFPLGGYPGDEDKARELFQKLDQSKTR
                     EDFIAAFEWLKARPETNGRVGAVGFCYGGGMVNFLATRLPDLAAGVPFYGSSPNVEDV
                     PKIKAPLLVQSAEVDERINASWPAFEAALKAANVRYERFLYPGTQHGFNNDTTPRYDA
                     AAAKLAWERTLAHLNKYVR"
     gene            9861..10232
                     /locus_tag="PKK95_06040"
     CDS             9861..10232
                     /locus_tag="PKK95_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17806.1"
                     /translation="MMRWPAAARQVGLVDQIFTSWNPLTGWMRLFGRCEARSGIAAFD
                     RLVEQVMGEQPYRSARRVCWITDNGSAHRGAKGDRRLQTRWPNVIPIHTPCHASWLNL
                     IPRRGFPTGGNNGQLGTGRGW"
     gene            complement(10395..10470)
                     /locus_tag="PKK95_06045"
     tRNA            complement(10395..10470)
                     /locus_tag="PKK95_06045"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10435..10437),aa:Ala,seq:ggc)
     gene            10783..>12244
                     /locus_tag="PKK95_06050"
     CDS             10783..>12244
                     /locus_tag="PKK95_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOC17807.1"
                     /translation="MFRRRFWLALALTVPTLVWGHMLPRALGYTPPAVPGANAIAPLF
                     GTAVFLVGGFVFIRGALGELRARQPGMMTLVALAITVAFGFSVAVTLGFRGMPLWEEL
                     ATLVTIMLLGHWIEMRSIARASGALNELAKLLPDVATRVAGDRLEEVPIAQLREGDLV
                     LVRPGQAVPADGVVREGETSVNEAMVTGESNPVPKGPGARVIGGTVNGSGAVRVEVTG
                     TGDRTALAGIARLVGQAQSSRSRAQALADRAAFGLTVVALAAGLVTLAAWIAIGDDIG
                     YAVERLVAVLVIACPHALGLAIPLVVAISTSLGAASGLLVRDRRGLEEARRLQVVVFD
                     KTGTLTRGEFGVVDVAAASGLDPREVLRLAAALEHDSEHPIARGIVRSAEEQGLAAVP
                     PQAFEALPGKGASARVEGRTLFAGGPALLAHLGLAVEGSLLEAARRAQSGGRAAIYLA
                     ERSGVLGVLTVADVVGPESRAAVGRLRSQGIAVVMLT"
BASE COUNT         1802 a         4283 c         4393 g         1766 t
ORIGIN      
        1 cctgcacctg cacaccaccg cctcggatgg ccgctgcccg ccgcgggaga tcgcggccgt
       61 ggcccggcgg gccggtctgg gcgtcatcgc cgtgacggat cacgacacgg tggccggtct
      121 tcgcgaggcc gccgaaggca cccggtcgca gggtatccgc ctggtgagcg gcatcgagat
      181 cactgccgtc caggaggacg ccgacgtcca cgtcctcggc tacttcttcg accccgaggc
      241 ccgcgatctc gggctgttcc tcgcgtcgca gcgtggcgac cggttgcgcc ggctgcgcgc
      301 gatggccgcg aagctcgcag cactcggcat gccgctcgac gtcgaccgca tcctcgacgc
      361 cgccggcggc gcaagcagcg gccgggccgt cgggcggccg atgctggcga gagcgctggt
      421 ggacgccggg tatctcgaga gccgccgcct cgcgttcgac ctctacctgg ccgaggggca
      481 gcccgcctgg gtgccgcgcc gggctccctc gccggccgag gtcgtcgagg tcatccacca
      541 ggcgggcgga ctggcgtcgc tcgcgcaccc cggcctgctc gggcgggacg agtggatccc
      601 tcccctggcc tcctcgggcc tcgacgcgct cgaggtctac tacagcgacc actcgccgga
      661 gctgaccatc cactatcgcg agctggcgtc tcggttgaag cttggcatga ccggcgggtc
      721 cgactatcac ggcgacccgg cgcacggctc cgagcgcccc ggcggctgcg tgctgccgcc
      781 agaggcgttc gaagggctcg aggcgctgcg gtcgcacagg gacgggacgc aatgacgatc
      841 acctttgccc ccgaggctcg cgaagcggtg cgggcctggc ttgcacgcga cccctcgaag
      901 ccggcgttgc ggctgttctt cgccggcggc tgcggcgccc tcggctacca gctcaccccc
      961 gcggccctcg aaccccggcc cggcgagcag gcgatcgagg tggacggggt cacgctgtac
     1021 gccgacttca agtcgtccac cgatctcgac ggcgcccgca tcgagctcgg cgagtcgccc
     1081 gacgacatcg tcctggtcca cgactcctgc gtcgtcggcg ggatgtgctg agagcagcgg
     1141 acagctgacc gcggattggg attgggattg aacgtcctcg agctgcgcgg catcgtgaag
     1201 gcccacggcg ggctgcggcc gttccggctg cgccggctcg cggtggcgcg cggcgaggtg
     1261 gtggtgatcg agggccccga cgcggcgcag gctgccgtcc tggtggatct gctgaccggc
     1321 accacgctgc cggatcaggg agaggtgctc gtcgacgggc gcaacacgcg gacgatcgag
     1381 agccaggacg agtggctggc gttcctcgag cggttcggcc tttccggcga gcgggtcgtc
     1441 ctgctgcccg agctcaccct cgttcagaac ctcgcgattc cgctgacgct ctccgtcgag
     1501 ccgattccca cgtcaattcg cccccgcgtg gacgccctgg ccgaggaggc tggcctgccc
     1561 gcgggccggc tcgagacggc cgtggctcgc gccggcccgg cggagcgggc gcgcgtccgc
     1621 ctcggccgcg cgatcgcgca cgatccggcg gtgctgctgc tcgagcaccc gacggctgga
     1681 atgagcgagg cggaggcgag ctcgttcggc gccgatctcc gtgcgatggc gtcgaggcgc
     1741 ggactgacgg tggttgccgc cagcgcggac cggaggttca gcggcgccgc cgcgacgcgc
     1801 gccctcgcct ggacggcggc aaccggcgag ctgatccgtt cgggcgggtc cctgcgccgc
     1861 ctgttcgggt tttgaatcga gaggctacac tgctgccgta tgcttcgcag ggatctcgtc
     1921 aggaacgcgt caccaaccgt gtcaaaagcc atgcacttga gccgaatcgt cccgtcccgc
     1981 tgtggaacac cttttgataa tcccccctgt cagccctcac caccggaggg cgtcgacgga
     2041 gggaaacgcg gatgccgacc ggaacgatca agcgactcgt cagggaccgg ggattcgggt
     2101 tcattcggga cgacggcggt caggagtggt tcttccaccg cagcgcggtg gcgcagggag
     2161 cattcgacca gctcaacgag ggccagcgcg tcgacttcga cgaggagccg tcgaacaagg
     2221 ggcctcgggc gaacaacatc cggatcgaag cgtaggacgg gcgcggcgtc gtgacggggc
     2281 cgggctgcca tcgcctggtg ctgttcgaca tcgacggcac gctgatcagg accggagggg
     2341 cgggcagccg cgcgctgaac cgcgcgttcg aggagtgggc agggatcccc gatggcctgg
     2401 acgggatccc ggtccccggg cgcaccgata ccatcatcct cgccgacttg ctccgccggc
     2461 acggccttgg agcgagccag tcggcgctcg gcactctcca ggacatctac ttccgccgcc
     2521 tggccgagga gttggctggg ctgccggccg gcgtggggcc gcttcctggc gtcgtcgcgc
     2581 tgctcgacgc cctcgaggcg gagccgcgcc ttgcgacggg gctgctcacc ggcaaccaca
     2641 ccacgggcgc caggctgaag ctcgagcggt acgggctctg gcactacttc cggttcgggg
     2701 cgttcggcga ggacgcggcc gacaggaacg acctcgtgcc ggtggcgctg gagcgggctg
     2761 ccgcggtggg gctgagggcc gcctcggccg ccgaggcggt tgtcgtcggc gacacggcgc
     2821 tcgacgtggc gtgcgggcag gcccacggcg cgcgcccgat cggcgtggcg acgggcgggt
     2881 tcagcgagga cgtgttagaa gaagcgggcg ccgatctcgt gctgccggat ctctccgaca
     2941 cggcgagaat cctggagtgg ctgactgggc cgagtcgaat ggcggaagcg cctgggagtc
     3001 gaacccagcc cccccgcaga gcggggagcg accggttttg aagaccggga gggccaccgg
     3061 gccccgttcg cttccggcct catcttcgca cacttcggac ctgaaccggt gaggcgtcga
     3121 gcagagcgtg accgacgcgc cgacacgcgg cacgtgaacg ggcacacgtc actcgtggac
     3181 ggcgcgccac gctttccttg ttggggaagt gcggggtgtg aaacgccgcc ggtgccgccg
     3241 ccgccggccg gggtgaacgg gagcccggct cccggtgcga cgggagccgg gggacgaggt
     3301 cgcgagcgac gctatccttc gatcagccgc acttctccgg cccgcggacc cttcgccgac
     3361 tcctcgggcg tgaactccac gcgctggccc tcgcgcagca gctcgaacac ggcgccgcga
     3421 acggaactgc ggtggaagaa gtgctcgatg cccccttcat ccctgatgaa gccaaacccc
     3481 ttgtcgatca acagacgagc gatggtcccg gtcggcatac cctgtcctcc gatgaacgca
     3541 cccccaagaa catcccaggg gccacaggca gctgtgacgg cgccacgcgc gcctcacagt
     3601 cacaaatgcc gccgacctga acctacgaca ccgactgccc ggggtcggcg gcccgaatcc
     3661 cgggtacgag gtagaacaac gcgacaacaa gacaacgggt ctgggcccag ctcgggatca
     3721 gcaacccccg ctgattacgg cgggattatc acgatgagct ggaaacggcc caagtttagc
     3781 aggtccgggt cggaagtcaa gcggggagtg gaggttacgt cttggggcct ccgggcgcct
     3841 tatcgggggc ggccccgggc gggttcgggt aggtggacca gaagtgccgg ccgcagcgag
     3901 ggcagaccca gtactggtcg ggcttccagg ggccgcccgg cgccgggtcc ttcatctcca
     3961 ttggggtgcc gcagcgctga cagttgatgc ggagctgctc ggccatgccc ttcgattcta
     4021 caatacgccc gatggtgggc gggatcatcc tcgcggcggg ggcctcgtcg cgcatggggc
     4081 gcgcgaaggc gcttctgccg tccggcgacg gtcggacctt tctcgagtgc ctggcctcgg
     4141 gcttctccga ggccggcctc gatccccttc tcgtggtggc cgggtcgcac gccgacgaga
     4201 tcgcggccct cgtccgggcg aagtcgctgc cgctccacct tgtcgtcaac ccgcggcccg
     4261 agcgggggca gctctcgtcc cttgtcgccg ggctcgatgc cctggccggc cgcgcgatcg
     4321 acgcggtgct cgtcacgccg gtggaccacc ccctcgtttc ccccgcgacc gtacgccgcg
     4381 tcgtggatgc ctggcgggaa tctggcgcgc tggtcgtacg gcctgagcgc agcgggcgcc
     4441 acgggcaccc cgtggtgttt gccgccgcgc ttttcgacga actgcgccac gcggatcttt
     4501 ccgtcggcgc ccgggcggtc atcgctcgtc actccgcaga ggtgctcgac gttcccgttg
     4561 gcgaaccggg cgcgttcgag gacatcgaca cgccggacga ttaccgccgc ctggtcggcc
     4621 aggcgctgcc cccgcgctag agaggtccgg ggatacctcc ctcgcgttct tcagcggcct
     4681 gctgttagaa cagccggaag atcgccgcgg tgaggagggt ggggccgatc gcccccgcca
     4741 acaggccgag cggcgacacg gcgccgccga agaaccggcg cagcagggcc tgtcccgcgg
     4801 ggttcggagc gttcgcgatc actgtcaggc ctccgcccac cacggccccc tgcacgaccg
     4861 ccagcctcag ttcttcgccg aggttcggga cgagcgttgc gaggtacgtg atcagggcgt
     4921 tgtcgttgaa cgcggtcagc accatgctgc cggcgaacag cggaaaggcg gtcaggcttc
     4981 cgagcaccgg tgcgatccac catccctgca ggccgccgtg gatgacgagg ccgccgagga
     5041 agaacccgac gagcagcggc gtgcgcagct cgagccgctg ctggtacatc ggcgtcgcca
     5101 gggcgaaccc cacgaagaag aggaacccgc caatgaacag cgcggggtag tgcgcggtca
     5161 agaccgtcca cccgatgaag ccgaggtgta cgacggtgac ccaggccggc accggcagga
     5221 gcgcctcgcc gggagcggcc gcgtcgtcgg gctgctcgac ctcgtcgggc gacgggctcg
     5281 aggccagcct cgacagctcc ttccggaaca gcaggtagca ggcgagcgtc gagacggcga
     5341 tcgcgagcgc cgcgcgccac ccgaagtgca gcagcatgta gggggtgtcc cacgagaagg
     5401 gccgggcgac catcagcacg ggcggcgcgg cgaagtgggt gagggtgccg ccgatcgaca
     5461 cgttgacgaa cagcagcccg agcgtcgcgt acttcaaggc ggcgctcggc tcccgatcgt
     5521 agaactgacg cccgagcagc agcgcgcaga tcgtcatcgc cgccggctcg gtgatgaacg
     5581 agccgagcag cggaccgatc gtgaggatgc tgacccacca cgcggcgggc gtgccgccgc
     5641 ccgcgtccgc cagccgccgc agcgcacctt ccgcgaagac gaccaccggc cgcgtcgagg
     5701 cgagcgccat gatgacgacg acgaagaggg gctcggtgta gttgaccgtg ccgttgaagt
     5761 agcccttcgc ggtttcccac cccatcgaga gcgcgatggc ggcgaacagc accacggccc
     5821 acaggccgaa caccacctcg acctcgccca cgaagtgcag cagctcggcg agcacgctgg
     5881 gccgcgggcg ctcgccgcgg gcgcgggcgc gctcgtcgtg gcggtgctgc acctggtgcg
     5941 agaggcgcgc gaagcgcccg gcggcgaagg tgtgcacgat ggccagcagg aagatcgccg
     6001 tcgcgaccgc gttgaacgga tcggtctcga cccgcgaggc cagcgtggac agcagcgacg
     6061 aggggtcggg cggcggcggg tagctgtcga gcgggcgggg aaacgagcct ggcacgggag
     6121 cgatgctcat ccgtacacgt taccggaccc gggccttcgc gggcaatcgg agccgctcac
     6181 cgaccgcctc accggagata cgactgcatc gccgcccagc ccgccagaac cgggacgtcg
     6241 ccggcgctgc gcacgcccca ctgcgtgctc gaccaggtgc tgtaaccggt tgccgaccag
     6301 aggaagtacc aggtgcctgg gccgggccgc cacacagcca ggtcggcgcg cccgtcgccg
     6361 tcgtagtccg ccggcactgg cacatcgtcc agcgagccga ggccccaccg cacgtgcact
     6421 tgcgcgtcgg cggagtagcc gtcggccgac tgcaggatcc accaggtgcc ggtgcccggc
     6481 cgccacacgg ccaggtcggc gcgcccgtcg ccatcgtagt cggcaggcac gggcacgtcg
     6541 aggaggcggc cgctgcccca cgcgacggcc aggcgatcct cggtccggaa tcccgatgtc
     6601 gaggtgagga tccaccaggt gccggttccg ggccgccaca cggccaggtc ggcgcgcccg
     6661 tcgccgtcgt aatcggccgg caccggctgg tcggtcgcgg ctccccagcg gaccacgagc
     6721 gcggacgacc gctggtagcc gccgctcgag cggaggatga accacgttcc cgtcgcgggc
     6781 cgccacacgg ccaggtcggc gcgcccgtcg ccgtcgtagt cggccggcaa cgggacgtcc
     6841 ctcgagttgc cgcttcccca ttggacctgc atctgggggg aacccgcgga ccgcaggatg
     6901 aaccaggttc ccgaagacgg cctccacacg gccgcgtcca ggacgccgtc gccgtcgtag
     6961 tccgcatgca ccggcacgtc ggacgcggct ccgaagcgga tgctgtcggc gcggccctgg
     7021 atataccagc ggccattgga gcggcggaag accgtgggcc gggagcgccg cgcgtcggcg
     7081 gccgcgccaa gggtcgtaaa cgaccaggcg cccgagtcgc gaacgagcga gggatcgcgc
     7141 ccggtggcgt tcgtcctcgc gacgaccttc cagaacagcg tctgtcccgg tgagagcgcg
     7201 gccggcggcg cccaggagta ggtggccggg gggtcgttga ccatctgcgc ggcgacgcga
     7261 gcgaccggcg acagcgcggc gggcgacggt ccgaggtaca cgtcgtagct cgtcgcgaag
     7321 ggggccgcgc gccacgagag ggcggtggcc tccacgaccc ccgaggcgcc ggcctgcggg
     7381 gcggcgaggc gcggctggtc gggcgggccg ggcacgaacg gctcgaacgc ctcggcgtcg
     7441 ttccacatct ggtcgaagcg tgcggccatc gcctggtgcg cggccggctt ggttgcggcc
     7501 ggcacgaagt agttgtggtc gcgttgccag ttcgtcgcga cgttcgacga ggcgttggtg
     7561 gcgtagcggg acgtgaccag catcttcatg tgcgtcaggc ccgcgtgccg gcgctgcctg
     7621 atccgcacgc cggcggccca caggcgatcg aggttggcgt gggtgagcca gaactcgggc
     7681 cagcgccggt tgaggtactc ggccggctcg acgatgagcc ggacgggtac gccggagcgc
     7741 gctttttcga cgagcgcgcg cgtcaccgtc tcggaggtga gccggtagac gacgaagcgc
     7801 accaggcgtt cctcgtttgc gatctcgcgg gcgagccggt tgttgaacga cgcgccctgc
     7861 ccccagacga ggccggccgg catcggccgg tcgggctcga ggcgggccgt gttgacgagc
     7921 atcggcgccg gcgccgggta caggctccgg tagctcgagc acgcggactc gaggccgcag
     7981 gcctcgtccc agttcaagag gtacggcggg gccgtgccgc ggctctccgg ttccggggtc
     8041 gtgtcgttcc acatggcgtc gaacttcgtc aggaaggcgc cgaccagcga cggatcggtc
     8101 gtgaagagca ccgtctcgtc cgagtagttg tcctcagacg ccggcgcgag ctggttcggc
     8161 gtgtagttgg ccgacccgaa cgacaccagg cgctgcccct tgaagatcgc cgccttccag
     8221 tgcgcgatct ccgggaacga gcggggatgg gtgcgcacgc ggatcgggac gccctgactg
     8281 gcgagccagt agaactcgtt cctcgtgagc gggtcggtct cgaagagcgc gacccggtcg
     8341 ccgatcaagc gaacctggac gccgcgccgg aacgcgtccc tcagggcggt cgacaccatc
     8401 cgctcggtga ggagccacgc ggccatgtcg atcctcaccg tctcggcgcg gatggccgcc
     8461 acgagcacgc tcctcacgtt ttggacggcg ggaaagtaga tgcgatcggt ggtttgagct
     8521 gacgaggacg ccgcgaagga cgatgcgagg gtggcgaacg agacgaaggc ggcaacgcag
     8581 cgcatcggtg ctgcaggctg ttccatgtca ctcccgtgcg gccgcgcgga cgggcttcgg
     8641 cgccccgttt tgagcggcag agtcggttag gcgatccgac gcacgaagca gcaacacgtg
     8701 tgccgctcct cggccgtatc gacctagaat caccttgtgc aggtcgaacg gctgtcggtg
     8761 ttcgacatcc agcggtgtac ctcttgcacg tctcgcctcg cttcggctcg gcaggatgcg
     8821 gcgggccgtc cccagtgcaa cactcaccag gcttcgagga ggacaaagcg atgccacgca
     8881 agaccgccca cgatttcgac caggaactgc tgattctctt cgacgcgtac gtgcacggcc
     8941 tgatcgaccg gcgcgcgttc ctggacaagg ccggcaagtt cgccgtgggg ggcatgaccg
     9001 cggcgatgct gctcgaccag ctcagcccga agttcctcga ggcgcaggtg atcaagcccg
     9061 acgatcctcg catcaaggcg cagcacgtcg agtacgagtc gccgaagggt tccggcaaga
     9121 tgcgagggta cctggcacag ccggccaagc ccgccggccg gctgccgggc gtcctcgtga
     9181 tccacgagaa ccgcgggctg aacccgcaca tcgaggacgt cgcgcgccgc ctggcgctgg
     9241 agaacttcgt cgcgttcgcg cccgacgcgc tcttcccgct cggcggctac ccgggcgacg
     9301 aggacaaggc ccgcgagctg ttccagaagc tcgaccagtc gaagacgcgc gaggacttca
     9361 tcgccgcctt cgagtggctg aaggcccgcc ccgaaaccaa cggcagggtt ggcgccgtcg
     9421 gcttctgcta cggcgggggg atggtgaact tcctggcgac ccgcctgccc gacctcgcgg
     9481 ccggcgtgcc cttctacggc agctccccca acgtcgagga cgtgccgaag atcaaggcgc
     9541 cgctcctggt gcagtcggcg gaggtcgacg agcggatcaa cgccagctgg ccggcgttcg
     9601 aggcggcgct caaggcggcc aacgtgcgct acgagcggtt cctctatccc ggcacccagc
     9661 acggcttcaa caacgacacg acgccgcggt acgacgcggc cgcggcgaag ctggcctggg
     9721 agcggacgct cgcccacttg aacaagtacg tgcggtaacg agccgcggcc cgccattccc
     9781 cttgacggtc gaaaggagtg gaagcacgcc gctaagcgaa gcgagttggc gagcgcgaaa
     9841 cagcgcagga gctagcgtcg gtgatgcgtt ggccggcagc ggcgcggcag gtcggtttgg
     9901 tggatcagat cttcaccagt tggaacccat tgacgggctg gatgcgactc ttcgggcgct
     9961 gcgaagcgcg gagcggcatc gccgccttcg accggctcgt cgagcaggtg atgggtgaac
    10021 aaccctatcg ctcggcccga cgggtctgtt ggatcaccga caacggctcg gcccatcgcg
    10081 gcgcgaaagg ggaccgccgg cttcagacgc gctggcccaa cgtgatcccc attcacacgc
    10141 cctgtcatgc cagttggctc aacctcattc cgcgacgagg attcccgacg ggtggcaaca
    10201 acggccagct cggcaccggg agaggttggt agattttgcg ggctcgggcg gaccgattgg
    10261 gtcggctgtc cgtccttcgc gtggtgccag cgcgcttggc tcgccatccg aagctcgttc
    10321 tgtggcaggg acacgcccac cttcgcgctt cgcgctatgg tgggcatcct tcgcgtgcat
    10381 tcatgagcga aggatggtgg acctgagggg agtcgaaccc ctgacctcct gaatgccatt
    10441 caggcgcgct cccaactgcg ctacaggccc acccgaggga acttctaatc ctaccctacc
    10501 gcgaccatcc cgtcaaaggc tccgctagcg cgcctgctct cactgacggc ctggagcggc
    10561 ccatgctccg cttcttgcat ggcccggccg acccgccaac tcgaggagga ggactcgcgt
    10621 ccgccaagcg ttcgccccgc tcacggctcc ggcggggcag gcccgcatca ctctcaccac
    10681 ccgccttcgc caaacgctcg cttcgctcac ggctccggcg gggcaggccc cgagcaccct
    10741 gaccaccacg accacgacaa gcatgccggc cacagcgtcg acatgttccg ccgccgcttc
    10801 tggctcgccc tcgcgctgac ggtgccgacc ctcgtctggg gccacatgct gccgcgcgcg
    10861 ctcggctaca cgccgccggc cgtgccaggg gcgaacgcga tcgcgccgct gttcggcacg
    10921 gccgtcttcc tggttggggg cttcgtcttc atccgcggcg cgctggggga gcttcgcgcg
    10981 cgccagccgg gcatgatgac gctcgtggcg ctcgccatca ccgtcgcgtt cgggttcagc
    11041 gtcgccgtca ccctgggctt ccgcggcatg ccgctctggg aggagctggc gacgctggtg
    11101 acgatcatgc tgctcggcca ctggatcgag atgcgctcga tcgccagggc gagcggcgcg
    11161 ctcaacgagc tggcgaagct gcttcccgat gtcgccaccc gcgtcgccgg cgaccgcctc
    11221 gaagaggttc ccatcgcgca gctccgcgag ggcgacctcg tcctcgtgcg gccgggccag
    11281 gccgtcccgg ccgacggcgt cgtgcgcgag ggcgagacga gcgtgaacga ggcgatggtg
    11341 accggcgagt cgaacccggt gccgaagggg cccggtgcgc gcgtgatcgg cggtacggtg
    11401 aacgggtccg gcgccgtgcg cgtcgaggtc accggcaccg gcgatcggac cgccctggcc
    11461 ggcatcgcgc gcctcgtcgg gcaggcgcag tcgtcgcgct cgcgtgccca ggcgcttgcc
    11521 gaccgcgccg cattcggcct gaccgtcgtg gccctcgccg ccggcctggt cacgctcgcg
    11581 gcctggatcg ccatcggcga cgacatcggt tacgcagtcg agcggctcgt cgcggtgctg
    11641 gtcatcgcct gcccccacgc gctgggcctc gccatccccc tcgtcgtggc gatctccacc
    11701 tcgctcggcg ccgcgtccgg gctgctggtg cgcgaccgcc gcggcctcga ggaggcgcgc
    11761 cggctccagg tcgtcgtctt cgacaagacg ggcacgctca cccggggtga gttcggggtc
    11821 gtggatgtcg ccgcagcctc cggcctggat ccgcgcgagg tactgcgcct cgccgccgcc
    11881 ctcgagcacg actcggagca cccgatcgcg cgcggcatcg tccgcagcgc cgaagagcag
    11941 gggctggcgg ctgtcccgcc gcaggccttc gaggcgctcc ccggtaaggg agcgtcggcg
    12001 cgcgtggagg gccgaaccct cttcgccggc ggtccggccc tgctcgccca cctcggcctc
    12061 gcggtggaag gctccctgct cgaggccgcc cgccgggcgc aatccggtgg ccgcgccgcg
    12121 atctacctgg ccgagcgctc gggcgttctt ggcgtgctca cggtggccga cgtcgtgggc
    12181 cccgagtcgc gcgccgcggt gggccgcttg aggagccagg gcatcgcggt cgtgatgctc
    12241 accg
//
LOCUS       DAOPQX010000076        12216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6018_length_12216_cov_4.296275, whole genome shotgun sequence.
ACCESSION   DAOPQX010000076 DAOPQX010000000
VERSION     DAOPQX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12216
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6018_length_12216_cov_4.296275"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PKK95_06055"
     CDS             <1..1149
                     /locus_tag="PKK95_06055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC17808.1"
                     /translation="VHVLEISARQRSLNFAPGTNWSYSNTGYNLAAIVVSRVAGMPFA
                     EFCRRRLFEPLGLSRTSWRDDHGRIVKGRAVAYAPDGGEFRTHMPFEDAHGNGGLLTT
                     VGDLLRWNTRFDGEGPDAALAREQMEPATLATGEKLDYGLGLFLSTYNGAAEVGHGGS
                     TAGYEAYLARYPEHRLSVAVLCNVSGAGAEARARAVADLLLGTKPRAVRPAPVTPAFL
                     NSWVGLYRSTLTGRSFDIVREGDALRVAGGSGLVATGHGRFAVGDGAREIAFEDVRRA
                     VLRYPNGLEERYERVERAHPSGDDLRALEGVYASEEAEAVYGAVVQKGELKLWRRPNL
                     LIPLKPTYADAFDAPGLGAVRFHREAGRVVEMIISGERVWNLRFRRIQ"
     gene            complement(1173..2387)
                     /gene="ispG"
                     /locus_tag="PKK95_06060"
                     /gene_synonym="gcpE"
     CDS             complement(1173..2387)
                     /gene="ispG"
                     /locus_tag="PKK95_06060"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOC17809.1"
                     /translation="MARHQTVGVRVGGIQVGGGAPVVVQSMANTDTTDVEATVRQNVQ
                     LARAGSEMLRITVNLPEAAAAVPEIKRRLLDEGCTAPLIGDFHYNGHLLLTRYPECAR
                     ALDKYRINPGNVGSGRRRDEQFATICGVARDNGKPVRIGVNGGSLNQELVMRRMQENT
                     DRDQGLSSEEIINECMVISALESTELALAAGLRENQIIISCKVSRPPHLVAVYRDLAS
                     KTRQPLHLGLTEAGMGVKGLVWSSAAMGILLSEGIGDTIRVSLTPRPGGDRREEVYAA
                     CELLQGLGLRSFSPSVTACPGCGRTTSTTFQELAERIQGYVREKMPEWKARYAGVETM
                     TLAVMGCVVNGPGESRAASIGISLPGTGESPHCPVYIDGQHFTTLRGNYAELSDAFRK
                     LVDDYVETKYGG"
     gene            2625..3566
                     /locus_tag="PKK95_06065"
     CDS             2625..3566
                     /locus_tag="PKK95_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17810.1"
                     /translation="MRGLILGKRALFGAVVLAVCLGITGVASAQESVTTADIQRLQDD
                     VYDAGSDLSRLRSRDAALADRLQDDLDLLREEVIYLKVKLRREGGVSRSEYSELRDRI
                     GSLRQRARGESSGAPAETARQTPIPAPAPAPPAEASEPVRQGTITRPNEVPVGTELDV
                     RLQHRLSSDDAKVEDRFEATTMVDLVEGGKVLIPAGSLVRGVVSGVEKAGRLNRTGRL
                     TLSFDQITVGGRSYPIHATVTQAIESEGIRGEAGRIGAGAGVGAVIGGILGGFKGVLA
                     GILIGGGGTIAATEGKDVTLPAGTVLRLRFDSPLTVR"
     gene            complement(3638..4939)
                     /gene="hemL"
                     /locus_tag="PKK95_06070"
     CDS             complement(3638..4939)
                     /gene="hemL"
                     /locus_tag="PKK95_06070"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOC17811.1"
                     /translation="MTKTRRWTKSARLYTRAQRTLPGGVDSPVRAFSAVGGDPLFIRR
                     ARGAEIVDADGHRYVDYVMSWGTLIHGHAPRGLVTALARAARDGTSYGAPTELEVKLG
                     EKVRSLMPSVERVRFVNSGTEAAMSAVRLARAATGRDRIVKFEGCYHGHADPFLAKAG
                     SAATTHGTPSSPGVPRKTVQHTLVAKYNDVRSCERLCEGFGTQIAAIVVEPLAANMGI
                     VPPADGFLAGLRALCDRHGILLVFDEVISGFRVGPGGAQGLWGVRPDLTCLGRIIGGG
                     LPVGAYGGREDLMEFVSPSGPVYQSGTLAGNPLVMTAGSWSLGGLTRPLYRRLARLGA
                     MLATGLADAAREAGVPVQVNAVGSLLTPFFTDGAVRDYASALRANSLQYATFFRAMLA
                     RGVYLPPSQFESWFISAAHTEASVRKTIAAAREAMREVAEM"
     gene            complement(4936..6486)
                     /gene="cobA"
                     /locus_tag="PKK95_06075"
     CDS             complement(4936..6486)
                     /gene="cobA"
                     /locus_tag="PKK95_06075"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOC17812.1"
                     /translation="MEKTPTVSIIGAGPGDPALISLRGLRALAQADVVIFDHLVHKRL
                     LRHARPGAERIDVGPAAPQPLAQEAICYLLAEKAREGKVVARLKWGDPFVFDRGGEEA
                     IFLHEQGVPFEVIPGIPAAVGVPAYAGIPLTYPGAGDAVVFVRGFESEDGRRPDVDWA
                     SLGKIDGTLVCYCGPRQLPAILDVMLSHGWDPACPAALVVNGTTPSQETVSATLGELA
                     RQAREPRFREPAMLIVGRVIGLREHLRWFDARPLFGKRVVVTRPREQAVELVELLEGL
                     GASVIEAPAVRIVAPDDFQPLDEAIASVGQYDWIVFTSVNGVDYFLQRLQAGPYDARA
                     LAGVKLCAVGPGTAERLAKYWLKVDLMPGEYRAEAVVEALRGEGEIAGRKFLLPRTDI
                     AREVLGDELRRLGAEVTEVTAYRSVAVEPEREHEPDIFRMLLDRKVDVVTFTSASTVR
                     NFCRLYGTDAVADLLRSVAIASIGPVTAEAALQCDIHTSIMPSEYTIPALVRAIVEHF
                     ERAPSVAT"
     gene            complement(6565..7491)
                     /locus_tag="PKK95_06080"
     CDS             complement(6565..7491)
                     /locus_tag="PKK95_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC17813.1"
                     /translation="MSGRRRAFLLGLAAPLAVAAALAAQQQPAFRAGVEIVSLTVTVT
                     DAEGRYVTGLADGEFEVYEDGVRQDVAYFNRTNLPIALSLLLDTSSSMEGRIGTAQEA
                     AAGFARRLRPEDLGGLVDFDTRVVVAQQFTSDAARLEEAIRRTTAGGSTSLYNAIYVA
                     LKELRKVRARSEQELRRQAVVVLSDGEDTSSLVSYEEVLDLAKRSDTSIYTIGLRDPE
                     EPATKGFNEAEFVLRDLAQQTGGRSFFPERIDDLAAVYDRIAEELASQYVLGYVSKNS
                     KRDGAWRRVVVRVARPDLRARTKQGYFAPTPR"
     gene            complement(7488..8399)
                     /locus_tag="PKK95_06085"
     CDS             complement(7488..8399)
                     /locus_tag="PKK95_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17814.1"
                     /translation="MPLPLSPSRALSSLLLCATAALLAAPGLMGQEARGPRERSIYVT
                     VTGDDGRPVTGLPPSAFVVREDGRRREVLTAAPATGPATLALMVDNSIASEPFIADIR
                     RGLEAFVDRMGGKHPMAVTTIGDRPTIVQDYTLDKESLLKGVRRIFPLPGSAAYFVDG
                     VAELSRGLIARDFERAFILAILTEGPEFSDRRHSDTLPLLRESGAALEALVINTPGGP
                     DLGDDAARSRSIILDRGTSETGGDRIDLLTSMAVGDALAAFAARVEGQYKVIYARPDS
                     LIPPEKIEVSMARPGLTARGTPAKGSR"
     gene            8635..8991
                     /gene="ndhC"
                     /locus_tag="PKK95_06090"
     CDS             8635..8991
                     /gene="ndhC"
                     /locus_tag="PKK95_06090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016718997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOC17815.1"
                     /translation="MLEAYVPVLLFVMVAIGFAVVALVFASLVRPERYNKVKLEPYEC
                     GIETTGDARDRYSIRYYIVAMLFVIFDVETVFMFPWAVIMDRLLLFGLIEMIVFLFIL
                     IVGYAYAWKQGALEWA"
     gene            9037..9729
                     /locus_tag="PKK95_06095"
     CDS             9037..9729
                     /locus_tag="PKK95_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC17816.1"
                     /translation="MPENDQNPTGGAPRPPAAPAKPAAAAPAKAAGAAKEKAPPPPPG
                     PPDPPPPDGVAVPAFLEELQSTLPGAIAQLSYWVGDWTVIVAPGRLREVAAHLRDAEG
                     ARFDYCSDATAVDWLGRPGPRFDLVYSLYSTALRHRVRVKSRVDDGESSPSLAPLWPA
                     ANWLEREVFDMFGIRFEGHPDLRRILMPEEWQGHPQRKDYPLEGPGELILESPQDWLR
                     LGQSIKESETES"
     gene            9729..10853
                     /locus_tag="PKK95_06100"
     CDS             9729..10853
                     /locus_tag="PKK95_06100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOC17817.1"
                     /translation="MERDAGRSMFDTDELVINMGPQHPATHGVLRVVLKLDGEKVVDA
                     DVVIGYLHRGVEKLTENRDWTQIVLLTDRLDYVASAAANLAYVETVEKLMSIEVPRRA
                     RYVRTILAELQRLASHLVWLGSHAMDIGAITVFLYCLRERELILDLFEEYCGARLTYN
                     SMRIGGLPQDVPSGWLPKVLRFCDVQEEKLAEYETLLTHNRIWLQRTRGIGAITGQAA
                     IAVGLCGPSLRGSGVPRDVRKDEPYAAYDEIPFDIAVGANGDTYDRYLVRLEEMRQSM
                     RIVRQAVEGLPEGPVMAKVPRLIKPPAGETYHANESPKGEIGFFIASDGRSTSPYRLR
                     VRPPSFCNLQALPQLVRGHLVADVIAIIGTIDIVLGEVDR"
     gene            10896..11921
                     /gene="nuoH"
                     /locus_tag="PKK95_06105"
     CDS             10896..11921
                     /gene="nuoH"
                     /locus_tag="PKK95_06105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOC17818.1"
                     /translation="MIDLIVLPLIKIVFVLTGVLITVMYLVLLERKVQAWVQARLGPM
                     RVGPHGVLQPIADVVKLFVKEDVTPVRAEKWVFTLAPIIVLVPSLIAFAVIPFGPEVN
                     LFGRPVPLYITDLNVGLLYIVSVAAIGVYGIILGGWSSNSKYPLLGSLRSSAQLISYE
                     LAVTLTFVSVILAAGSLSMVQIVEAQHANGVWFAFVQPVALFIYGVGALAETNRNPFD
                     LPEAESELVAGFHTEYSGMRFALFFLAEYTNMIVVSAVATTLFFGGWLRPFPNVEWLS
                     FLGLVPAWIWFTLKTFALLYVFLWIRATLPRYRYDQLMQLGWKWLIPLAIANIVVTAA
                     VKVILGS"
     gene            11926..>12216
                     /locus_tag="PKK95_06110"
     CDS             11926..>12216
                     /locus_tag="PKK95_06110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOC17819.1"
                     /translation="MGEAIAFYTFAAMILGFGVLVITARNTVHSVLFLVANFLFVAAL
                     YVLLGAQFLAVIQVIVYAGGIVVLYLFVVMLVNLKRPPEAHRDRRRKTRLGLA"
BASE COUNT         1779 a         4210 c         4361 g         1866 t
ORIGIN      
        1 gtgcacgtgc tcgagatcag cgcgcggcag cggtcgttga acttcgcccc cggcacgaac
       61 tggtcctaca gcaacaccgg ctacaacctg gctgcgatcg tcgtttcccg ggtcgcgggg
      121 atgccgttcg ccgaattctg ccggcgccgc ctcttcgagc cgctcggcct gtcgcgcacc
      181 tcgtggcgcg acgaccacgg gaggatcgtc aagggacggg ccgtcgccta cgcccccgac
      241 ggcggggagt tccgcacgca catgccgttc gaggacgccc acgggaacgg cggcctgctg
      301 acgacggtcg gcgacctgct gcggtggaac acgaggttcg acggggaggg cccggacgcg
      361 gccctcgccc gcgagcagat ggagccggcg acgctggcga cgggcgagaa gctggactac
      421 gggctcgggc tgttcctgtc gacctacaac ggcgccgccg aggtcggcca cggcgggtcc
      481 acggccgggt acgaggcgta cctcgcgcgc taccccgagc accggctttc ggtcgcggtg
      541 ctgtgcaacg tgagcggtgc cggggccgag gcccgggcac gagccgtcgc cgacctcctg
      601 ctcggaacca agccgcgggc agtcaggccg gccccggtga cgccggcgtt cctgaactcc
      661 tgggtggggc tgtacaggag cacgctcacg gggcggtcgt tcgacatcgt tcgcgagggc
      721 gacgcgcttc gcgtggcggg aggctcgggg ctggtcgcca ccggccacgg ccggttcgcc
      781 gtcggggatg gcgcacgcga gatcgcgttc gaggacgtgc gaagggccgt cctccggtac
      841 ccgaacggcc tggaggagcg ttacgagcgc gtcgagcgcg cgcacccgtc gggcgacgac
      901 ctgcgcgccc tggaaggggt ctacgcgagc gaggaggccg aggcggtgta tggggcggtc
      961 gtgcagaagg gcgagctgaa gttgtggcgg cggcccaacc tgctgatccc gctgaagccg
     1021 acctacgccg acgccttcga cgcgccgggc ctcggcgccg tgcgcttcca ccgcgaggcg
     1081 ggccgggtcg tcgagatgat catcagcggc gaacgcgtct ggaacctgag gttcaggaga
     1141 atccagtagg ggcggccctt gtggccgccc tcctatcctc cgtacttcgt ctccacgtag
     1201 tcgtccacca gcttgcggaa cgcgtccgac agctccgcgt agttgccgcg cagcgtcgtg
     1261 aagtgctggc cgtcgatgta cacggggcag tgcggcgatt cgccggtgcc cggcaggctg
     1321 attccgatgc tggccgccct ggattcgccc ggtccgttca ccacgcaccc catcaccgcg
     1381 agcgtcatcg tctccacgcc ggcgtagcgc gccttccact ccggcatctt ctcgcgcacg
     1441 tatccctgga tgcgttcggc cagctcctgg aaggtggtgc tggtggtccg gccgcagccc
     1501 gggcacgccg tcacgctcgg ggagaaagag cgcagcccaa ggccctgcag caactcgcac
     1561 gcggcgtaga cctcctcgcg ccggtcgcct ccgggccgcg gcgtcagcga cacccggatc
     1621 gtgtcgccga tcccctcgct cagcaggatg cccatcgcgg ccgacgacca gacgagcccc
     1681 ttgacgccca ttcccgcctc ggtgagccca aggtgaagcg gttggcgcgt cttcgacgcg
     1741 aggtcgcggt agaccgcgac caaatggggc ggacgcgaga ccttgcacga gatgatgatc
     1801 tggttctcgc gcagcccggc cgcgagcgcc agctcggtgg actcgagtgc cgagatcacc
     1861 atgcactcgt tgatgatctc ctcggaggag agtccctggt cgcggtcggt gttctcctgc
     1921 atgcggcgca tcaccagttc ctggttcagc gagccgccgt tcacgccgat ccgcaccggc
     1981 ttcccgttgt cccgggcaac gccgcagatc gtggcgaact gctcgtcgcg ccggcgtccc
     2041 gaccccacgt tgcccgggtt gatgcggtac ttgtcgaggg cccgggcgca ctcggggtag
     2101 cgagtgagca gcaggtggcc gttgtagtgg aagtcgccga tgagcggcgc ggtgcaaccc
     2161 tcgtccagca gccgccgctt gatctcgggg acggccgccg cggcctcggg caggttcacc
     2221 gtgatgcgga gcatttccga cccggcccgg gcgagctgca cgttctgccg gacggtcgcc
     2281 tcgacgtcgg tcgtgtcggt gttggccatc gactgcacga cgacaggggc accgccgccc
     2341 acctggatgc ctccgacgcg cacgccgacg gtctgatgcc gagccatgcc acgatcatag
     2401 cccagtgaca cagtggcagc ggcacaggca gtggcccagt cagtggcaca ggccagttgc
     2461 ctcagccagt ggcacaggca tcccgcttgt gggtgaacaa gttggcgaca cagtggcaca
     2521 ggcgtcccgc ctgtgggcag gcgggtgtgg gcgggcgggt tggctccccg gcgcgttcgg
     2581 gatacactgt ctgagccata ttccagtcac agaggaggaa cgggatgcga ggcctgatcc
     2641 ttgggaagcg cgccttgttc ggcgcggtcg tgctggccgt gtgccttggg attaccggcg
     2701 tcgcgagcgc ccaggaatcc gtgacgacgg cggatatcca gcggctccag gacgacgtct
     2761 acgacgcggg gagcgatctg tcccggctgc gctcgcgtga cgcggcgctc gccgaccggc
     2821 tccaggacga tctcgacctg ctgagggaag aggtcatcta cctgaaggtg aagctgcgcc
     2881 gcgagggcgg cgtgtcacgc tccgagtaca gcgagctgag agaccggatc ggcagcctgc
     2941 gccagcgtgc ccgcggcgag agcagcgggg cgcccgccga gacggcccgc cagacgccga
     3001 tcccggcgcc cgccccggcg ccgcccgccg aggcgtccga gccggttcgc cagggcacca
     3061 tcacccgccc caacgaggtg cccgtcggga ccgagttgga cgtccgcctg cagcaccgcc
     3121 tcagctcgga cgacgcgaag gtggaggacc ggttcgaggc gaccaccatg gttgacctcg
     3181 tcgaaggcgg caaggtgctc atccctgccg gttcgctcgt gcggggagtc gtcagtgggg
     3241 tcgagaaggc cgggaggctg aaccggacgg gacggttgac gctgagcttc gaccagatca
     3301 ccgtcggagg ccgctcctac ccgatccacg ccaccgtgac gcaggcgatc gagagcgagg
     3361 ggattcgcgg cgaggctggc cggatcggcg cgggcgcggg cgtcggggcc gtcatcggcg
     3421 gcatcctcgg cgggttcaag ggcgtcctcg ccggaatcct catcggcggc ggcggcacca
     3481 tcgcggccac cgagggcaag gacgtcaccc tgccggcagg caccgtcctg cggctccggt
     3541 tcgactcgcc gctcacggtg cggtagcagg ccgccgcccg tcagctcgtt ccacccccct
     3601 gcatcctcga ggcggggcgc gcggcgcgcc ccgcccctta catctctgcc acctcgcgca
     3661 tcgcttcgcg ggcggccgcg atcgtcttgc ggaccgacgc ctcggtgtgg gcggccgaga
     3721 tgaaccagga ttcgaactgc gacggcggca ggtaaacccc tctcgcgagc atggctctga
     3781 agaacgttgc gtactgaagg ctgttcgccc ggagggcgga ggcgtagtcg cggaccgcgc
     3841 cgtcggtgaa gaagggcgtg agcagcgacc ccaccgcgtt gacctgtacc ggcacgccgg
     3901 cttcccgtgc ggcgtcggcc aggccggtgg ccagcatggc gccgagccgg gccaggcgcc
     3961 ggtacagcgg gcgcgtcaaa ccgccgagcg accacgagcc cgccgtcatg acgagcgggt
     4021 tgcccgccag cgtgcccgac tggtagaccg gccccgaagg cgacacgaat tccatcaggt
     4081 cctcgcgccc cccataggcg cccacgggca gtccgccgcc gatgatcctc cccaggcacg
     4141 tcaggtcggg ccgcacgccc cagaggcctt gcgctcctcc cggcccgacc cggaagcccg
     4201 agatcacctc gtcgaacacc agcaggatgc cgtggcggtc gcacagcgca cgcagcccgg
     4261 cgaggaagcc atcggctggg ggcacgatgc ccatgttcgc ggcgagcggc tcgacgacga
     4321 tggccgcgat ctgggtgccg aagccttcgc acagccgctc gcacgaccga acgtcgttgt
     4381 atttggccac cagcgtgtgc tgcacggtct tgcgcggcac cccggggctc gagggcgtgc
     4441 catgggtggt ggcggccgag ccggctttcg ccaggaaggg gtccgcgtgg ccgtggtagc
     4501 acccctcgaa cttcacgata cggtcgcggc cggtggccgc gcgcgccagc cggacggcgc
     4561 tcatcgcggc ctcggtgccg gagttgacga accggacacg ttcgaccgac ggcatgagcg
     4621 agcgcacctt ctcgcccagc ttgacctcga gctctgtcgg ggcgccgtaa ctcgtaccgt
     4681 ccctcgccgc gcgcgccagg gccgtgacga gccctcgggg cgcgtgtccg tgaattagcg
     4741 tcccccatga catcacgtaa tcgacgtagc ggtggccatc cgcgtccacg atctcggcgc
     4801 cgcgggcgcg ccggatgaag aggggatcgc cgccaaccgc gctgaaggcg cgaacagggc
     4861 tgtccacgcc tccgggcaga gtgcgctgcg cgcgcgtgta cagcctggcc gacttcgtcc
     4921 agcgtcgcgt cttcgtcatg tggccacaga gggtgcccgc tcgaagtgtt cgacgatggc
     4981 gcgcaccagc gcggggatcg tgtactccga gggcatgatc gacgtgtgga tgtcgcactg
     5041 cagcgcggct tcggccgtca ccggcccgat cgaggcgatg gcgaccgagc gcagcaggtc
     5101 ggccaccgcg tcggtcccgt agaggcggca gaagttgcgc acggtggacg cgctcgtgaa
     5161 ggtcaccacg tcaaccttgc ggtcgagcag catccggaag atgtcgggct cgtgctctcg
     5221 ctcgggctcg acggccacgc tgcgatacgc ggtaacctcc gtgacctcgg cgccgaggcg
     5281 cctgagttcg tcgccgagca cctcgcgcgc gatgtcggtg cggggcagca ggaacttcct
     5341 gcccgcgatc tcgccctcgc cgcgcagcgc ctcgaccacg gcctcggcgc ggtactcgcc
     5401 gggcatcagg tccaccttca accagtactt ggcgagccgc tccgcggtgc ccgggcccac
     5461 ggcgcacagc ttcaccccgg ccagggcgcg cgcgtcgtac gggcccgcct gcaggcgctg
     5521 gaggaagtag tcgaccccgt tgacgctcgt gaacacgatc cagtcgtact gccccaccga
     5581 ggcgatcgcc tcgtccaggg gctggaagtc gtcgggggcg acgatgcgga cggcgggcgc
     5641 ctcgatgacg gaagcgccga ggccctcgag cagctccacc agctcgacgg cctgctcgcg
     5701 ggggcgcgtc acgaccaccc gtttgccgaa caaggggcgc gcgtcgaacc agcgcaggtg
     5761 ctcgcggagg ccaatcacgc ggccgacgat cagcattgcc ggctcgcgga accggggctc
     5821 gcgcgcctgg cgggccagtt cgccgagcgt cgcggacacc gtctcctgcg aaggcgtcgt
     5881 cccgttcacc acgagcgcag ccgggcaggc cggatcccac ccgtgcgaca gcatgacgtc
     5941 gaggatggcc ggcagctggc gcggaccgca gtagcacacc agcgtgccgt cgatcttgcc
     6001 gaggctggcc cagtcaacgt ccggccggcg gccgtcctcg ctctcgaagc cgcggacgaa
     6061 aacgaccgcg tcccctgcgc ccgggtaggt gagggggatg ccggcgtacg ccggcacccc
     6121 gaccgccgcc gggatgccag ggatcacctc gaaggggacg ccctgctcgt ggaggaagat
     6181 ggcttcctcg ccgccgcggt cgaagacgaa ggggtcgccc cacttcaggc gcgccaccac
     6241 cttgccctcg cgcgccttct cggccagcag gtagcagatc gcttcctggg ccagcggttg
     6301 gggagcggcc ggccccacgt cgatccgctc ggcgccgggc cgtgcgtgcc gcagcaggcg
     6361 cttgtgtacc aggtggtcga agatgacgac gtccgcctgc gccaacgcgc gcaagccgcg
     6421 cagcgagatc agcgccgggt cgccgggacc cgccccgatg atggagacgg ttggcgtttt
     6481 ctccatgaca ggagactacc accgggccac gcccccgggt caccgaaaac gccacggccg
     6541 ggccgtcccc gtgggccgcg tcgtctagcg tggcgtcggc gcgaagtacc cctgcttggt
     6601 tcgcgccctg aggtcggggc gggcgactcg cacgacgacg cggcgccagg cgccgtcgcg
     6661 cttgctgttc ttcgacacgt agccgagaac gtactggctc gccagctcct cggcaatccg
     6721 gtcgtacacg gccgccaggt cgtcaatccg ctcggggaag aacgagcggc cgcccgtctg
     6781 ctgcgccagg tcccggagca cgaactcggc ctcgttgaag cccttggtcg ccggttcctc
     6841 gggatcgcga aggccgatcg tgtagatcga ggtgtccgag cgcttcgcga ggtcgagcac
     6901 ctcctcgtag gaaaccaggc tgctcgtgtc ctcgccgtcc gagagcacga cgaccgcctg
     6961 gcggcgcagc tcctgctccg agcgggcgcg taccttcctc agctccttca gcgccacgta
     7021 gatcgcgttg tacagcgacg tggacccgcc ggcggtcgtc cggcggatcg cctcctcgag
     7081 gcgcgccgcg tcgctggtga actgctgcgc gaccacgacc cgcgtgtcga agtccacgag
     7141 ccccccgagg tcctcgggcc tcaggcgccg ggcgaagccc gccgcggcct cctgcgcggt
     7201 gccgatccgt ccctccatgc tggagctggt gtcgagcagc agcgagagcg cgatcgggag
     7261 attggtgcgg ttgaagtagg cgacgtcctg gcgcacgccg tcctcgtaca cctcgaactc
     7321 gccgtcggcc aggccggtca cgtagcggcc ctcggcgtcg gtcaccgtca cggtgagcga
     7381 gacgatctcg acgcccgccc ggaaggcagg ctgctgctgc gccgccaggg cggcggccac
     7441 ggcaagcggg gcggcgagcc cgagaaggaa cgcccggcga cggccgctca tcgggatccc
     7501 ttggccggcg tcccgcgcgc ggtgaggccg ggccgcgcca tgctcacttc gatcttctcg
     7561 ggcgggatga gcgagtcggg ccgcgcgtag atgaccttgt actgcccctc gacgcgcgcc
     7621 gcgaaggccg cgagcgcgtc gccgaccgcc atgctggtca gcaggtcgat gcggtcgccg
     7681 ccggtctccg aggtgccccg gtccaggatg atgctgcggc tcctggccgc atcgtcgccg
     7741 aggtcggggc ctcccggcgt gttgatgacc agcgcctcga gcgcagcgcc gctctcgcgc
     7801 agcaagggga gggtgtcgct gtggcggcga tcgctgaatt cgggcccctc ggtcaggatg
     7861 gccaggataa aggcgcgctc gaagtcgcgg gcgatcaggc cccgcgacag ctcggccacc
     7921 ccgtcgacga agtacgccgc gctgcctggg agcgggaaga tgcggcgcac ccccttgagc
     7981 aggctttcct tgtccaaggt gtagtcctgg acgatcgtcg ggcgatcccc gatcgtcgtc
     8041 acggccatcg ggtgcttgcc gcccatccga tcgacgaacg cctcgaggcc ccggcggatg
     8101 tcggcgatga acggctcgga tgcgatcgag ttgtccacca tcagcgcgag cgtggcgggc
     8161 ccggtggccg gggcggccgt cagcacctcg cggcggcggc catcctcgcg cacgacgaac
     8221 gccgagggtg ggaggccggt cacggggcgc ccgtcgtcgc ccgtcacggt gacgtagatc
     8281 gaccgctcgc gcggcccccg ggcctcctgc cccatcaggc caggggcggc aaggagggca
     8341 gcggttgcac agaggagcaa cgatgacagg gcgcggctgg gcgagagggg aaggggcatg
     8401 atggcagtat agggacgagt ctcgggaggc gtcaaccctg gacgtcctca actctttgaa
     8461 agaactggac ttgtggacgc gagacgaggt cgtccgggcg gcgcgctttc ttgaccgcct
     8521 ggcgggccgc gtgctataga tacaaagttc taccagctcg attcccgcga gagacacgaa
     8581 cctgcgaatc cgccagccct ggcccccggc ggccgtggtg cggacggccc gaagatgctc
     8641 gaagcctacg ttcccgtgct cctgttcgtg atggtggcga tcgggttcgc cgtcgtcgcc
     8701 ctggtgttcg cgtcgctcgt gcgccccgag cgctacaaca aggtgaagct cgagccgtac
     8761 gagtgcggca tcgagaccac cggcgacgcc cgcgaccggt acagcatccg ctactacatc
     8821 gtcgcgatgc tgttcgtcat cttcgacgtg gagacggtct tcatgttccc ctgggcggtc
     8881 atcatggacc ggctgctgct gttcggcctg atcgagatga tcgtcttcct cttcatcctc
     8941 atcgtcggct acgcgtacgc gtggaagcag ggcgcgctcg agtgggcgta gggcccgcct
     9001 gggtgccggg ctccggctac ccgcacacga gattccatgc ctgagaacga ccagaacccg
     9061 acgggcggcg cgccgcgtcc cccggcggcg ccggccaagc cggccgcggc cgctccggcg
     9121 aaggccgctg gggccgccaa ggagaaggcg ccgccgcctc cacccggccc tcccgacccg
     9181 ccgccgcccg acggcgtcgc ggtgccggcg ttcctcgagg aactgcagtc cacgctgccc
     9241 ggggcgatcg cgcagctctc ctactgggtc ggcgactgga cggtcatcgt ggccccgggg
     9301 cggctgcgtg aggtcgccgc gcatctccgg gacgccgagg gggcccggtt cgactactgc
     9361 tcggacgcga ccgccgtgga ctggctgggg cggccggggc cgcgcttcga cctggtctac
     9421 agtctctact cgacggcgct gcgccaccgg gtgcgggtga agtcgcgggt ggacgacggg
     9481 gagagcagcc cgtcgctggc gccgctctgg ccggcggcga actggctgga gcgggaggtc
     9541 ttcgacatgt tcgggatccg cttcgagggc caccccgacc tgcgccggat cctgatgccc
     9601 gaggagtggc agggccaccc gcagcgcaag gactacccgc tcgaggggcc cggcgagctg
     9661 atcctcgaga gcccgcagga ctggctgcgg ctgggccagt cgatcaagga atcggagacg
     9721 gagtcgtaat ggaacgggac gccggccgat cgatgttcga caccgacgag ctcgtcatca
     9781 acatggggcc gcagcacccg gccacgcacg gggtgctgcg cgtcgtcctg aagctggatg
     9841 gcgagaaggt ggtggacgcc gacgtggtga tcggctacct gcaccgcggc gtcgagaagc
     9901 tgacggagaa ccgcgactgg acgcagatcg tgctgctgac cgaccgcctc gactacgtcg
     9961 cctcggcggc ggcgaacctg gcgtacgtcg agacggtcga gaagctgatg tcgatcgagg
    10021 tgccgcgccg cgcgcggtac gtgcggacga tcctcgcgga gctgcagcgg ctggcgagcc
    10081 acctggtgtg gctgggcagc cacgcgatgg acatcggggc aatcacggtc ttcctgtact
    10141 gcctccggga gcgcgagctg atcctcgacc tgttcgagga gtactgcggg gcgcgcctga
    10201 cctacaactc gatgcggatc ggcggcctgc cgcaggatgt cccctcgggg tggctgccga
    10261 aggtgcttcg attctgcgac gtgcaggaag agaagctcgc cgagtacgag accttgctga
    10321 cgcacaaccg catctggctg cagcgcacgc gcgggatcgg ggcgatcacg gggcaggcgg
    10381 cgatcgcggt cgggctgtgc ggcccctcgc tgcgggggtc tggcgtgccg cgggacgtgc
    10441 ggaaggacga gccgtacgcg gcctacgacg agatcccgtt cgacatcgcc gtcggggcca
    10501 acggcgacac gtacgaccgc tacctggtcc gcctcgagga gatgcggcag tcgatgcgca
    10561 tcgtccggca ggcggtcgag gggctgcccg agggaccggt gatggcgaag gtgccccggc
    10621 tgatcaagcc gccggccggc gagacctatc acgcgaacga gtcgccgaag ggggagatcg
    10681 gcttcttcat cgcgagcgac ggccggtcca cgagcccgta ccggctgcgc gtccgcccgc
    10741 cgtcgttctg caacctccag gcgctgccgc agctggttcg ggggcacctg gtggccgacg
    10801 tcatcgcgat catcggcacc atcgacatcg tgctcggcga ggtggatcgg tagcgccgcg
    10861 cggaggccat ccctccgcct cacggatcga gtggcatgat tgacctgatt gtcctcccgc
    10921 tgatcaagat cgtcttcgtc ctcaccgggg tgctcatcac ggtgatgtac ctcgtgctcc
    10981 tggagcggaa ggtccaggcc tgggtgcagg cgcgcctcgg gccgatgcgc gtcggccccc
    11041 acggcgtgct gcagccgatc gccgacgtgg tgaagctgtt cgtcaaggag gacgtgacgc
    11101 ccgtccgcgc cgagaagtgg gtcttcacgc tggcgccgat catcgtgctg gtcccgtcgc
    11161 tgatcgcttt cgcggtcatc ccgttcgggc ccgaagtgaa cctgttcggc cggccggtgc
    11221 cgctctacat cacggacctg aacgtcgggc tgctgtacat cgtgtcggtg gcggcgatcg
    11281 gcgtgtacgg gatcatcctg ggcgggtggt cgtcgaactc gaagtacccg ctgctcggca
    11341 gcctgcggtc gtcggcgcag ctgatcagct acgagctggc ggtgacgctg accttcgtga
    11401 gcgtgatcct ggcggccggc agcctgagca tggtgcagat cgtcgaggcg cagcacgcca
    11461 acggcgtctg gttcgcgttc gtgcagccgg tcgcgctctt catctacggc gttggggcgc
    11521 tggccgaaac caaccgcaac cccttcgacc tgcccgaggc cgagtcggag ctggtggcgg
    11581 ggttccacac cgagtacagc gggatgcggt tcgcgctgtt cttcctggcc gagtacacca
    11641 acatgatcgt cgtctcggcg gtcgcgacga cgctgttctt cggcgggtgg ctgcgcccgt
    11701 tcccgaacgt cgagtggctc tcgttcctcg ggctggtgcc ggcctggatc tggttcacgc
    11761 tgaagacctt tgccctgctg tacgtcttcc tgtggatccg ggcgacgctg ccgcgctacc
    11821 gctacgacca gctgatgcag ctcggctgga agtggctgat cccgctcgcc atcgcgaaca
    11881 tcgtcgttac cgccgcggtg aaggtgatcc tggggagctg atgagatggg agaagcgatc
    11941 gccttctaca cgtttgccgc gatgatcctc ggcttcgggg tcctggtgat caccgcgcgg
    12001 aacaccgtgc acagcgtgct gttcctcgtg gcgaacttcc tgttcgtcgc ggccctttac
    12061 gtgctgctcg gcgcccagtt cctcgcggtc atccaggtga tcgtgtacgc cggcggcatc
    12121 gtcgtcctgt acctgttcgt cgtgatgctg gtgaacctga agcgcccgcc ggaggcgcac
    12181 cgggaccgcc ggcggaagac aaggctcggc ctcgcc
//
LOCUS       DAOPQX010000077        12015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6139_length_12015_cov_4.169064, whole genome shotgun sequence.
ACCESSION   DAOPQX010000077 DAOPQX010000000
VERSION     DAOPQX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6139_length_12015_cov_4.169064"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            189..1610
                     /locus_tag="PKK95_06115"
     CDS             189..1610
                     /locus_tag="PKK95_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17820.1"
                     /translation="MNTRLRLLIFVLAVPLLAALSSSAARDRWDERWRAGLGREFRAF
                     GHRAGPAVMARYSLETICADPRRAAAVPPCRVYNRLTTLIVAGAATSGLGVLWLGVMA
                     AAGAAAARRRRFAARAFRPLTYASAVVLVVLAAAQGLLAVQALSLSGRLLLPGVVPAA
                     FGRFAGLAAALLVGLLAAQAARSIKRLSAVPYAFARVLGAGEGSKLRASVSRLCEPGG
                     PPEDLELAVGLAPAVFVVPGPTDSLDGRRTGPLLHFPLSLARILTVPECEALLAAQLF
                     RLRDGEARSLGRFTGGWLRLRDEAGRQHSAPALWRTALLPVAWWLTFLADAFAPAATA
                     AWRDEEEGGDRTAIATGGAEAYLVALLKVHAFAPAWDAALQAMADAVSQGEQYASAPA
                     LFASIVTANAEPSRVEWALGGLAAPNGEVRGGLSRWGSADLQRVAAAALDVQPEVAAT
                     TLVGDVAVRLEADLTDARHALLQ"
     gene            1719..3542
                     /gene="typA"
                     /locus_tag="PKK95_06120"
     CDS             1719..3542
                     /gene="typA"
                     /locus_tag="PKK95_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOC17821.1"
                     /translation="MPGTGKSTPSIANALRRNVAIIAHVDHGKTTLVDGLLRQSGVFR
                     ANERVAERVMDNIDLERERGITILAKNTAVRYGAHLINIVDTPGHADFGGEVERTLSM
                     VDGVLLLVDASEGPLPQTRFVLRKALERRLPPIVVINKIDRADARPQQVLNEIYDLFI
                     DLDASEALLEFPVLYANARAGTASADPSVQGSDLRPLFEAMLQHVPPPLGDPDGRLQA
                     LVANLDSSDYLGRIAIGRVFNGRIRVGDAISVCKLDGRVEPTKVTKLFAFEGLKRVDI
                     PEAAAGDIICVAGIADITIGETIADAEAPSPIAPIAVDEPTVSMIFGVNTSPLAGREG
                     QYVTSRNLRDRLARELLGNVSIRVEDTDSPEQVKVVGRGELQLSILIEMMRREGFELQ
                     VSRPQIVTRERDGVVFEPVEDLVIDVPEEFQGVVIAQVGSRRGAMTRMVNNGSGRVRL
                     EFHLPTRGLIGFRSQFLTDTRGTGIMNHLFSAWERWHGPIPARPTGALVADRAGAATA
                     YAIYNLQERGEIFVSPGTEVYEGMIVGENARPNDLDVNVTKEKKQTNMRASTADEAIR
                     LIPPRLLNLEQAIEFIKDDELVEITPRTIRLRKKVLAANMR"
     gene            complement(3615..4394)
                     /locus_tag="PKK95_06125"
     CDS             complement(3615..4394)
                     /locus_tag="PKK95_06125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC17822.1"
                     /translation="MTTDETPRADDPYSRVDYRRMVRWPERIEREWPFLRRALGPSGR
                     ILDLGCGTGEHSRFLAAQGFEVVGVDASPAMIAKATDTPVPANLRFVEGDIGEVAELV
                     DGPFDGAICLGNTLPHLPDRRALTGFAAGLRRLLREKAVLVLQVLNYEKIFATGQRFL
                     PLNFRPGNEPGEEVVFLRLMTLADDGSLVFTPSTLRLRPGAEPPLEVVSARNVRLRGW
                     RRKELEEALDAAGFDHLDLFGTVGDTPYLPLDSPDLVIVAR"
     gene            4635..5843
                     /locus_tag="PKK95_06130"
     CDS             4635..5843
                     /locus_tag="PKK95_06130"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOC17823.1"
                     /translation="MMARVAVGVMAAGMVLAASSGALGQTPVLNPTTVEFDPSPDHST
                     LGSDGQPVVQRYELRMYLLGRSDPTVTSDLGKPAAGADGKIRVDFSTRTTPWPPPHGD
                     YEARVAAVGPSGVGQSDSSNPFSFSFQTACGYTLSATSAALPATGGTTSVALSTGSGC
                     AWTVTGRPSWLTAAAASGVGSATLTFTATANASTSARTASLAAGGKTFTVTQAGAACS
                     YGLSATSATVGPEGGSATATLTTGSGCSWTVTGAPSWVTLGAGSGVGGATLTFTASPN
                     TSSSARSASMTVGGRSFVLNQQAGTCLFLLSPASVVVGGNGNSGSLTMTTSTGCKWTA
                     SSSVPWITLKTSAGTGSGNISYTVAKNPSTAGRVGTIQAGGKTSTLTQGVAARPSAPK
                     KPKVSLTVGE"
     gene            5907..6230
                     /locus_tag="PKK95_06135"
     CDS             5907..6230
                     /locus_tag="PKK95_06135"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOC17824.1"
                     /translation="MTPPAVAGTERRKAARVTYFAEAWLEGLDVSRTSVRLGDLSTEG
                     AFVDARTVLPPGTIARLRFRLEEREIGVMTEVRYAIPAIGMGLHFLDLTPEDRAAIQA
                     FVAAC"
     gene            6312..9158
                     /locus_tag="PKK95_06140"
     CDS             6312..9158
                     /locus_tag="PKK95_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HOC17825.1"
                     /translation="MPEPAASLLDDLSRTVGRDSVLTRPIDLAAYASDASVYRITPAA
                     VVRPRDLVQVRSVFEVARGHGLPVAFRAAGTSLSGQAVTPGVLIDLSRSWRGIDVLDG
                     GARVRVEPGVVGAAVNARLLRHGARIGPDPASIQACMVGGILANNSSGMCCGVAQNAY
                     HTLDSLVFMLPSGTSIDTAEPRADDRLREAEPSIHAGLADLRREVFGTAGLADRIRSK
                     YRMKNTTGYSLNALVDFERPVEMLRHLLIGSEGTLAFIASAVLWTIPDRPVKHTALLL
                     FPGIREACAAITPLKEAGAAALEVMDRAAMRSVERQPGVPAALQGLPPDAAGLLVEFQ
                     APAESDRPALHEAASRAVRGLSLLDAPAFTDDPIEQLGLWKVRQGMFPSVGATRPRGT
                     AVLIEDVAFPVERLAEAVTDLQALLRAHGYADAIIFGHAREGNLHFVISQSFNEPEAI
                     TRYARFMDELVRLVAAGYGGALKAEHGTGRNMAPFVETEWGADAYRVMRGIKRLVDPG
                     GLLNPGVLITEDPGEHLRNLKGLPIVEPEVDACIECGYCEPRCPSRDLTLTPRQRIVV
                     RRQVARLEASGGGHARAAIEVDFPYEALDTCAVDGLCATQCPVGIDTGQLTKRLRASR
                     HSPAARRAARWVGRHMALVERSLRGGLAATAAIRNVNGGWVLSLASRLPRALGIGSPL
                     WIEPMPGPARWRRPDGAGAAGAAAVYFPSCLSRALGHLPGEPRELTLVEAMTRVSARA
                     GAPVFVPADADGHCCGVPFSSKGYQEAHAQTVNRTIEALWRWSGEGRLPVVVDTSPCT
                     YGLRSSRPVLTAANQARFDGLRILDGVEHAASLLGRLAVRRRQRRVVLHPVCSLVKMN
                     LVPELERIARACADQVVLPIDAGCCGFAGDRGWLFPELTASATSAEAREATSAPADGY
                     YSSSRTCEIGMARATGEIYRSYLYLLEWATREGD"
     gene            complement(9163..9717)
                     /locus_tag="PKK95_06145"
     CDS             complement(9163..9717)
                     /locus_tag="PKK95_06145"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOC17826.1"
                     /translation="MRRLVLVLAALAIAVPAVAGTLAGVTLPDSISVGGRTLALNGMG
                     VRTKVFFKVYVGGLYLEKKTTDAAEVIQSDAARRLVLQFVRDVDRNQITEAFDESVRN
                     NAAAQAASLKAEVAQFLAALEPFKSGEQMTITYLPGTGTTISVKGADKVTIPGLPFGQ
                     LVFSMWLGPKPPSGDLKKGLLGIR"
     gene            complement(9823..11319)
                     /locus_tag="PKK95_06150"
     CDS             complement(9823..11319)
                     /locus_tag="PKK95_06150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC17827.1"
                     /translation="MPAVDRVSTPPRAGFPRTFWVANVMELFERAAYYGMNSVLAVYL
                     TSSVAEGGIGASVEAVGFLQSIVYAATYVVPILGGALADRYGYRRMLMVAFSLLAAGY
                     FAAGYMSAYALVFLALLVMATGSGLFKPIISGTIARTTDETNSAFGFGIYYWMINLGA
                     FLAPLVVSVLKGFSWQYVFMASALYTGLMLLPTIFAYADPPRPESTKKLKDVLLGAAE
                     VLGDARFMLMIVVYSGFWILYFQNFGSVLWYLRDFVDRGPVSAAMTSLFRSVGLPWTF
                     VFDAEHVTVINAGTIILLQVLVSRAVKTTPALPTMVTGMAIGALGFLCLAASLNPWVF
                     VLGIAVFSIGEMTAHPKYYSFVGLVAPADRKAVYMGFAFLYGVIGSLLGSSIGAFLYG
                     RFMTPVVGTPAAAGRARLFWLMFAVLDLVAAAGLILFAKRFGTDTPETRRGARTVMDV
                     VYGAILLLGGWFLYTAFTSSPVNARTAVQALIFLFLGIGGLVMNRRRQ"
     gene            complement(11369..>12015)
                     /locus_tag="PKK95_06155"
     CDS             complement(11369..>12015)
                     /locus_tag="PKK95_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOC17828.1"
                     /translation="ARDRKGAWRVQRYRSRLIPVTGDLADHPAVAAVVQKYWDPIAKV
                     YGEVVGQAAGEFTSRGDDAAEYHLMADAVRAEFQAEISLENAGGIRAPLLRGPITRGD
                     LVTLDPFDNTVIRFEATGRQIREILARYTPWVSGARYRVQGRRLVEATIGGAPIDDDR
                     VYKGVTNSYFAGYALKGVPTEDTGRARLETLVGYIRRQGTVEPVYDGRRVVARD"
BASE COUNT         1770 a         4199 c         4301 g         1745 t
ORIGIN      
        1 ggttgatcct cgaggacgag aaggtgatgg gcacgatcca catcgccttc ggggacaaca
       61 agtcgatggg aggcagcgtc cgggtggcca gccacctcga cggcctcatc cgctggccga
      121 ccgtctggct ggacgacgag aagatcatgg aggatggcac gctgctcgtc gcctgacccg
      181 gcccccggat gaacacgcgg ctccgcctcc tgatcttcgt gctcgccgtc cctctgctgg
      241 ccgcgctgtc ctcgtcggcg gcgcgcgacc gctgggacga gcgctggaga gcggggctcg
      301 gccgcgagtt ccgcgccttc gggcaccggg cgggtccggc cgtgatggcg cgctactcgc
      361 tcgagacgat ctgcgccgac ccgcgccgcg cggcggccgt cccgccctgc cgcgtctaca
      421 accgtctcac caccctcatc gtggccggcg ccgcgacgtc cgggctgggc gtgctctggc
      481 tgggcgtgat ggccgcggcg ggagcggccg ccgcccgccg gcggcgtttc gccgcccgcg
      541 ccttccggcc gctcacctac gcatcggccg tggttttggt cgtcctggcc gcggcgcaag
      601 ggctgctcgc ggtccaggcc ttgtcgctgt cgggccggct gctgctgccg ggggtggtcc
      661 cggcggcgtt cgggcgcttc gcggggctgg cggccgccct cctggtgggg ctgctcgccg
      721 cgcaggccgc gcgctccatc aagcggctct cagcggtgcc atacgccttc gcccgggtcc
      781 tcggggccgg cgaaggctcg aagctgcggg cctcggtgag ccgactgtgc gaaccgggcg
      841 gtccgccgga ggacctggaa ctcgcggtcg gcctggcccc tgccgtgttc gtcgtgcccg
      901 gccccacgga ttcactggac gggcggagga cagggccgct gctgcatttc ccgctgtctc
      961 tcgcccgcat cctcaccgtt cccgagtgcg aggcgcttct ggccgcccag ctgttccggc
     1021 tccgcgacgg ggaagcccgg tcgctggggc gcttcacggg gggctggctg cggctgcgcg
     1081 acgaggcggg ccgccaacac tcggcgcccg cgctctggcg gaccgcgctg ctgcccgtcg
     1141 cctggtggct gacgttcctt gccgacgcct tcgcgcccgc ggcgacagcc gcctggcgtg
     1201 acgaggaaga gggtggcgac cggacggcga tcgcgaccgg tggggctgag gcgtacctcg
     1261 tcgccctgct caaggttcac gccttcgcgc cggcgtggga cgcggcgctc caggcaatgg
     1321 ccgacgcggt gtcacagggc gagcagtacg ccagcgcgcc ggcgctgttc gcgtcgatcg
     1381 tgaccgccaa cgcggaaccc tcgcgcgtgg agtgggcgct gggcggtttg gccgccccca
     1441 acggcgaggt gcgtggcggg ctctcccggt ggggaagcgc cgacctccag cgggttgcgg
     1501 ccgccgccct cgacgtgcag ccggaggtcg ccgccaccac gctggtcggc gacgtcgccg
     1561 tgcgcctgga agccgacctg accgacgccc gccacgccct cctccagtag aacaggcgtc
     1621 tcgcctgttc gtgagagggc gtctcgcctg ttcgagaggg cgtcccgcct gttcgtgaga
     1681 gggcgtccgc gtctccctgc ctgtgctacg ctggtcggat gcccggaacc ggaaagagca
     1741 cgccttcaat cgccaacgcg ctgcggcgaa acgtcgccat catcgcgcac gtcgatcacg
     1801 gaaagaccac gctggtggac ggcctgcttc gccaaagtgg ggttttccgc gccaacgagc
     1861 gggtcgccga gcgcgtgatg gacaacatcg acctcgagcg cgagcgcggg atcacgatcc
     1921 tcgccaagaa caccgcggtc cggtatggcg cgcacctcat caacatcgtg gacacacccg
     1981 ggcacgccga tttcggcggc gaggtcgagc gcacgctctc gatggtggac ggcgtgctcc
     2041 tgctggtgga cgcgtcggaa gggccgctgc cgcagacgcg cttcgtgctg cggaaggccc
     2101 tcgagcggcg cctcccgccg atcgtcgtga tcaacaagat cgaccgggcg gacgcccgtc
     2161 cccagcaggt actgaacgag atctacgacc tgttcatcga cctcgacgcc tccgaggcgc
     2221 tgctcgagtt cccggtgctc tacgccaacg cgcgcgccgg caccgcgtcg gccgacccgt
     2281 ccgtgcaggg aagcgacctg cggccgctgt tcgaggccat gctgcagcac gtcccgccgc
     2341 cactcgggga ccccgacgga cggctgcagg cgctggtggc caacctcgac tcgagcgact
     2401 atctcgggcg gattgcgatc ggccgggtgt tcaacggccg gatccgggtc ggggacgcca
     2461 tctcggtctg caagctcgac ggccgggtcg agcccacgaa ggtcaccaag ctgttcgcct
     2521 tcgaggggct caagcgggtg gacatccccg aggcagccgc cggcgacatc atctgcgtcg
     2581 ccgggatcgc cgacatcacg atcggcgaga cgatcgcgga cgccgaggcc ccgtccccga
     2641 tcgcgccgat cgcggtggac gagccgacgg tgtcgatgat cttcggcgtg aacacctcgc
     2701 ccctggccgg ccgggagggg cagtacgtga cgtcgaggaa cctgcgcgac cgcctggcgc
     2761 gcgagctgct cggcaacgtg tcgatcaggg tcgaggacac cgactcgccc gagcaggtga
     2821 aggtcgtcgg gcgcggcgag ctgcagctgt cgatcctgat cgagatgatg cgccgcgaag
     2881 gattcgagct gcaggtctcg aggccgcaaa tcgtcacgcg cgagcgcgac ggcgtcgtgt
     2941 tcgagccggt cgaggacctc gtgatcgacg tcccggaaga gttccagggc gtggtgatcg
     3001 cgcaggtcgg gtcgcgccgc ggcgcgatga cgcgcatggt gaacaacggc agcggccgcg
     3061 tgcgcctcga gttccacctt ccgacgcgcg gcctgatcgg cttccgttcg cagttcctta
     3121 ccgacaccag ggggaccggg atcatgaacc acctgttctc cgcctgggag aggtggcacg
     3181 ggccgatccc cgccaggccg acgggcgcgc tggtggccga ccgcgccggg gccgccaccg
     3241 cctatgccat ctacaacctg caggagcgcg gcgagatttt cgtcagcccg ggaaccgagg
     3301 tctacgaggg gatgatcgtc ggcgagaacg cgcggcccaa cgacctcgac gtcaacgtga
     3361 cgaaagagaa gaagcagacc aacatgcggg cgtccaccgc cgacgaggcg attcgcctga
     3421 tcccgccccg cctgctgaac ctggagcagg cgatcgagtt catcaaggac gacgagctcg
     3481 tggagatcac gccgcgcacc atccgcctgc ggaagaaggt gctggccgcc aacatgcgat
     3541 gaagcccggc aggccggttc gggcgacacg ctcacggcct ctcttcacgc cagggagcgc
     3601 ggcgagcccc gggctcagcg cgcgacgatg acgaggtcgg gagagtcgag ggggaggtac
     3661 ggcgtgtcgc ccaccgtgcc gaacaggtcg aggtgatcga agccggcggc gtcgagcgcc
     3721 tcctccagtt cctttcggcg ccacccccga aggcgcacgt tgcgcgcgga gacgacctcg
     3781 agcggcggct cggcgcccgg acggaggcgc agggtggacg gggtgaacac aaggctgccg
     3841 tcgtccgcga gggtcatcag gcgcaggaac accacttcct cgcccggctc gttccccggc
     3901 cggaagttga gcggcaggaa gcgctgcccg gtcgcaaaga tcttctcgta gttcaggacc
     3961 tgcagcacca gcaccgcctt ctcgcgcagg aggcggcgca ggccggccgc gaagccggtc
     4021 agcgcgcggc gatccggcag gtgcggcagg gtgttgccca ggcagatcgc gccgtcgaac
     4081 ggcccgtcca ccagctccgc cacctcgccg atgtctccct cgacgaaccg gaggttcgcg
     4141 ggcaccggcg tgtcggtggc cttggcgatc atggcagggg aggcgtcgac gccgaccacc
     4201 tcgaagccct gcgcggccag gaagcggctg tgctcgcccg tgccgcaccc caggtccagg
     4261 atgcggccgc tggggccgag ggctcgccgc aggaagggcc actcgcgctc gatgcgttcg
     4321 ggccaccgca ccatccggcg gtaatccacc cgcgagtagg ggtcgtcggc gcgcggcgtc
     4381 tcgtcggtcg tcatcggctg tcctcttgac tgtatcccgc cgattcgctc gcggcaaggc
     4441 gcaccaaagc tggatcccac ccacaaaacc gtaagaacgg aggcgctgag ggcagtcgct
     4501 ggcctgccgt ctgtgtcgtt aaggtccttt gaatcaatgg gatatgaccg tcaagctcgg
     4561 caggcacatc aattgcggac agccggggag gtcaagccgc gcctggcggc caaggctggg
     4621 agagcaacgt gagcatgatg gcccgagtgg cggtcggggt catggcggcg gggatggtgc
     4681 tggcagcctc cagcggcgcc ctgggccaga cgcccgtact caatcccacc accgtggagt
     4741 tcgatccgtc ccctgaccac tccaccctcg gatctgacgg acagccggtc gtccagcggt
     4801 acgagctgcg gatgtacttg ctcgggaggt ccgatcccac cgtgaccagc gacctcggca
     4861 agccggccgc tggcgctgac gggaagatcc gcgtggactt ctcgacccga accacgccct
     4921 ggcccccgcc ccacggcgac tacgaggcgc gggtggcggc ggttggcccg agcggcgtcg
     4981 gccagagcga cagctcgaac cccttctcct tctcgttcca gaccgcctgc ggctacacgt
     5041 tgtctgcgac gagcgctgcg cttccggcga ccggcggaac aacgagtgtg gccctgtcga
     5101 cgggctcggg ctgtgcgtgg acggtcaccg gcaggccaag ctggctgacg gcagcggcgg
     5161 ccagcggcgt gggcagcgcg acgcttacct tcacggccac ggcgaacgct tcaacgagcg
     5221 cgcgtaccgc ctcgctggcg gccgggggga agaccttcac ggtgacgcag gcgggggcgg
     5281 cgtgcagcta cggcctgtcc gcgacgagcg ccacggttgg ccctgaaggc ggttcagcaa
     5341 ccgccacgct gaccaccggc tcggggtgtt cgtggacggt gaccggcgcg ccgagctggg
     5401 tgacgctcgg cgcgggttcc ggcgtgggcg gtgcgacgct gaccttcaca gcctcgccga
     5461 acacctcgtc gagcgcgcgc agcgcgtcga tgacggtggg cgggcggagc ttcgtcctca
     5521 accagcaggc cgggacctgc ctgttcctgc tctcgccggc gtcggtcgtt gtcggcggca
     5581 acggcaacag cggctcgctc accatgacca cctccaccgg gtgcaagtgg accgcctcgt
     5641 cgagcgtccc ctggatcacg ctgaagacca gcgccggcac gggatcgggc aacatctcgt
     5701 acacggtcgc gaagaacccg tcgaccgccg gccgggtggg cacgatccag gcgggcggaa
     5761 agacatccac cctcacgcag ggagtggcgg cgagaccctc ggccccgaag aagccgaaag
     5821 tgtcgctcac ggtgggtgag tgagccgcgt cagcggcgcg aatcagtagt ggatctcgag
     5881 gcctcgggct gctaggctgt gcgtcgatga caccacctgc cgtggcgggg acagaacgcc
     5941 gcaaggctgc gcgggtgacg tacttcgccg aggcctggct cgaggggctc gacgtgagcc
     6001 ggacgtcggt gcgcctcggt gacctcagca ccgagggggc cttcgtcgac gcccggaccg
     6061 ttttgccgcc ggggacgatc gcccggctgc gattccgcct cgaagagcgg gagatcggcg
     6121 tgatgaccga ggtccgctac gccatcccgg cgatcgggat ggggttgcac ttcctcgacc
     6181 tgacgcccga ggaccgcgcg gccatccagg ctttcgtcgc ggcctgttga ggccggcagc
     6241 cgccgcacgt ccctcccggt tggcggtggc gcccgttctg ccatcccgcc tcccttcgct
     6301 atacttgcgg cgtgcccgaa cctgccgcct cgctgctcga tgacctttca cgcacggtcg
     6361 gccgcgattc cgtcctgacg cgtcccatcg atctggccgc ctacgcctcg gacgccagcg
     6421 tgtaccgcat cacgccggcc gccgtggtgc gtccgcggga cctggtccag gttcgctcgg
     6481 tgttcgaggt cgcacgcggc cacggactgc ccgtcgcgtt tcgcgcggcg gggacgagtc
     6541 tctcgggcca ggccgtcacc cccggggtgc tgatcgacct gtcgcggtcc tggcggggga
     6601 tcgacgtgct cgacggcggc gcgcgcgtgc gggtcgagcc gggggtcgtg ggagccgcgg
     6661 tgaacgcccg gctgctccgg cacggcgcgc gcatcgggcc cgaccccgcg tccatccagg
     6721 cgtgcatggt gggcggaatc ctggcgaaca actccagcgg catgtgctgc ggcgtcgcgc
     6781 agaacgccta ccacacgctc gactcgctgg tgttcatgct gccgtcgggc acctcgatcg
     6841 acacggccga gccgcgcgcc gacgatcggc tgcgcgaggc ggagccgtcg atccacgcag
     6901 gtctggccga cctgcggcgg gaggtgttcg ggacggcggg cctggccgat cgcatccggt
     6961 cgaagtaccg gatgaagaac accaccgggt actccctgaa cgccctcgtg gacttcgagc
     7021 ggccggtcga gatgctccgg cacctgctga tcggctctga aggtacgctg gccttcatcg
     7081 cgagcgccgt gctctggacg attcccgatc gtcccgtgaa gcacacggcc ctgctgttgt
     7141 tcccgggcat ccgcgaggcg tgcgccgcca tcacgccgct gaaggaggcc ggggcggcag
     7201 ccctcgaggt gatggaccgg gcggcgatgc gatcggtgga gcggcagccc ggcgtgccgg
     7261 ctgcgctgca ggggctgcct cccgacgcgg cgggactgct cgtcgagttc caggcgccgg
     7321 ccgaatcgga ccgccccgcc ctgcacgagg cggcgtcacg ggcagtgcgc ggcctgtcgc
     7381 tgctcgacgc gcccgccttc accgacgatc ccatcgagca gctcgggctc tggaaggttc
     7441 gccaggggat gttcccgtcg gttggcgcga cacgcccccg cggcacggcg gtgctgatcg
     7501 aggacgtggc gttcccggtg gagcggctcg ccgaggcggt gaccgacctg caggcactgc
     7561 ttcgcgcgca cgggtacgcc gacgcgatca tcttcggcca cgcacgcgag ggcaacctgc
     7621 acttcgtcat cagccagtcg ttcaacgaac cggaggcgat cacccggtac gcgcgcttca
     7681 tggacgaact cgtccggctg gtggccgccg gctacggggg ggcgttgaag gccgagcacg
     7741 gcaccgggcg aaacatggcg cccttcgtgg agacggagtg gggtgccgac gcttaccgcg
     7801 tgatgcgggg gatcaagcgc ctcgtcgatc ccggcggcct gctcaaccct ggcgtactga
     7861 tcaccgagga tcccggcgag cacctgcgga acctcaaggg gctcccgatc gtggagcccg
     7921 aggtcgacgc gtgcatcgag tgcggctact gcgagcctcg ctgcccaagc cgcgatctca
     7981 ccctgacacc gcgccagcgc atcgtcgtcc ggcggcaggt cgcgcggctc gaggcgagcg
     8041 gtggcgggca cgcccgcgcc gccatcgagg tcgatttccc ctacgaggcg ctcgacacct
     8101 gtgcggtgga tggcctgtgc gccacgcagt gcccggtggg catcgacacc gggcagctga
     8161 ccaagcggct gcgggcctcg cgccactcgc cggccgcgcg gcgggcggcc cgctgggtgg
     8221 gccgccacat ggcgctcgtc gagcggtcgc tgcggggcgg cctcgcggca acagccgcga
     8281 tcaggaatgt taacggaggc tgggtgctgt cgctggccag ccggctgccc agggcgctgg
     8341 gaatcgggag cccgctctgg atcgagccga tgcctggccc cgcgcgctgg cggcgtccgg
     8401 acggtgccgg cgctgccggc gcggccgcgg tgtacttccc gtcgtgcctg tcgcgggcgc
     8461 tcgggcacct gcccggcgag ccacgcgagt tgacgctggt cgaggcgatg accagggtgt
     8521 ccgcacgcgc gggtgcccct gtcttcgttc ccgccgacgc cgacgggcac tgctgcggcg
     8581 ttccgttctc ttcgaagggg taccaggagg cgcacgccca aacggtgaac cgcacgatcg
     8641 aggcgctctg gcggtggtcc ggcgaaggcc ggctgccggt ggtggtcgac acgagcccgt
     8701 gcacgtacgg gctgcgatcg tcgcgcccgg tgctgacggc agccaaccag gcgcgcttcg
     8761 atggcctgcg gatcctggac ggggtcgagc acgccgcgtc gctgctgggg cgcctggcgg
     8821 ttcgccggcg gcagcgccgg gtggttctgc accccgtctg ctcgctggtg aagatgaacc
     8881 tggtgcccga gctggagcgg attgcccgcg cctgcgccga ccaggtcgtg ctaccgatcg
     8941 acgcggggtg ctgcggcttc gcgggagacc gcgggtggct gttccccgag ttgacagcct
     9001 cggcgacctc ggccgaggcg cgcgaggcga cctcggcgcc tgccgacggc tactactcga
     9061 gcagccgcac ctgcgagatc gggatggcgc gcgcgaccgg cgaaatctac cggtcgtacc
     9121 tctacctgct ggagtgggcg accagggaag gggactgacg ccttaccgga tccccagcag
     9181 ccccttcttc aagtcgccgc tcggcggctt gggcccgagc cacatcgaga acaccagctg
     9241 gccgaacggc aggccgggaa tggtcacctt gtcggcgcct ttcacgctga tcgtcgtgcc
     9301 ggtgccgggc aggtaggtga tcgtcatctg ctcgcccgac ttgaacggct cgagggccgc
     9361 caggaactgc gcgacctccg ccttcagcga cgccgcctgc gcggccgcgt tgttcctcac
     9421 cgactcgtcg aaggcctcgg tgatctggtt ccgatccacg tcgcgcacga actggagcac
     9481 caggcgcctg gccgcgtccg actggatgac ctcggccgcg tcggtggtct tcttctccag
     9541 gtagaggccg cccacgtaca ccttgaagaa caccttggtc cgcaccccca tcccgttcag
     9601 cgccagcgtc cggccgccga ccgagatcga gtcgggaagg gtgacgcccg cgagcgtccc
     9661 cgcgacggcc gggacggcga ttgcgagcgc ggcgagcacg agcaccagtc tgcgcatctt
     9721 cacctccgtg gaccgatgtc cggacatgag cggaacgccc cgcacgggtg gacgcctgtc
     9781 tgcacaggcg ggacgccagc ctgcacaggc gggacgcctg tgctactggc ggcgacggtt
     9841 catcacgagg ccgccgatac cgaggaacag gaagatcagc gcctgcaccg cggtccgggc
     9901 gttcaccggc gaggaggtga acgcggtgta caggaaccac ccgccgagca gcaggatggc
     9961 cccgtagacg acgtccatca ccgtccgggc cccccggcga gtctccggcg tgtctgtccc
    10021 gaaccgcttg gcgaacagga tgaggcccgc cgccgcgacg aggtcgagca cggcgaacat
    10081 cagccagaag aggcgagccc gtccggcggc cgccggcgtc cccaccaccg gggtcatgaa
    10141 ccgcccgtag aggaacgccc cgatgctcga ccccagcagg gagccgatca cgccgtagag
    10201 gaacgcgaac cccatgtaga cggccttccg gtcggccggc gcgaccagcc ccacgaagct
    10261 gtagtacttc gggtgcgccg tcatctcgcc gatcgagaac accgcgatcc cgagcacgaa
    10321 gacccacggg ttcagcgacg cggccaggca gagaaagccg agcgcgccga tggccatgcc
    10381 ggtgaccatc gtcggcagag cgggggtggt cttgaccgcg cggctgacga gaacctgcag
    10441 caggatgatc gtcccggcgt tgatgacggt gacgtgctcg gcgtcgaaga cgaacgtcca
    10501 ggggaggccg accgaccgga acagcgaggt catcgccgcg ctgaccgggc cgcgatcgac
    10561 gaagtcgcgc aggtaccaca gcaccgatcc gaagttctgg aagtacagga tccagaaccc
    10621 cgagtacacg acgatcatga gcatgaagcg ggcgtcgcca agcacctcgg cggccccgag
    10681 caggacgtcc ttcagcttct tggtgctctc gggccggggc gggtcggcgt aggcgaagat
    10741 cgtcggcagc agcatcagcc cggtgtagag ggccgacgcc atgaacacgt actgccagga
    10801 gaagcccttc aggacgctga cgaccagagg tgccaggaag gcgccgaggt tgatcatcca
    10861 gtagtagatc ccgaacccga acgccgagtt ggtctcgtcg gtcgtgcgcg cgatcgtgcc
    10921 cgagatgatc ggcttgaaga ggcccgagcc ggtggccatc accagcaggg cgaggaacac
    10981 cagcgcgtag gccgacatgt agccggccgc gaagtacccc gctgccagca gcgagaaggc
    11041 taccatcagc atccgccggt agccgtagcg gtcggccagc gcgccgccca ggatcggcac
    11101 cacgtaggtg gccgcgtaga cgatgctctg gaggaagccg acggcctcga cgctggcgcc
    11161 gatgcccccc tcggccacgc tcgacgtcag gtagacggcg agcaccgagt tcatgccgta
    11221 gtaggcggcg cgctcgaaca gctccatgac gttcgcgacc cagaacgtcc tcgggaaccc
    11281 ggcgcgcggg ggagtgctga cccggtcgac tgctggcatg gcgctccttc gcgtcagagg
    11341 ctgaggggcg gcggcagaga cgagcctgtc agtcgcgggc caccacgcgg cggccgtcgt
    11401 agaccggctc cacggtaccc tggcggcgaa tgtacccgac gagcgtctcg agcctggcgc
    11461 gccccgtgtc ctcggtgggc actcccttga gcgcgtaccc ggcgaagtag gagttggtca
    11521 cgcccttgta gacgcggtcg tcgtcgatcg gcgcgccgcc gatcgtggct tccaccagcc
    11581 ggcgcccctg cacccggtac cgcgccccgg agacccacgg cgtgtagcgg gccaggattt
    11641 cgcggatctg ccgtcccgtc gcctcgaacc ggatcaccgt gttgtcgaag gggtcgagcg
    11701 tgacgaggtc gccgcgggtg atcggcccgc gcagcagcgg cgcccggatg ccgcccgcgt
    11761 tctcgaggga gatctcggcc tggaactcgg cccgtaccgc gtcggccatc aggtgatact
    11821 cggcggcgtc gtctccccgg ctggtgaact cgccggccgc ctggccgacg acctcgccgt
    11881 agaccttcgc gatcgggtcc cagtacttct gcacgacggc ggccactgcg gggtggtcgg
    11941 ccaggtcgcc ggtgaccggg atcagccgcg agcgatagcg ctgcacccgc cacgcccctt
    12001 tccggtcgcg ggcca
//
LOCUS       DAOPQX010000078        11828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6250_length_11828_cov_4.503525, whole genome shotgun sequence.
ACCESSION   DAOPQX010000078 DAOPQX010000000
VERSION     DAOPQX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11828
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6250_length_11828_cov_4.503525"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /gene="ileS"
                     /locus_tag="PKK95_06160"
     CDS             complement(<1..1726)
                     /gene="ileS"
                     /locus_tag="PKK95_06160"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOC17829.1"
                     /translation="MADWKDTLNLPRTGFPMKANLQSAEPAAIARWEATGLYEQLRRR
                     RKGAPTFILHDGPPYANGPLHLGTALNKILKDFVVRSRSMAGFDAPYVPGWDCHGLPI
                     ERNVDRELGERRRAMTTVEVRRACREYATRFVDIQRAGFKRLGVAGDWDSPYLTMAYG
                     YQATIVRSLARFVEQGRVYKGKKPVHWCIVDRTALAEAEVEYEDHTSPSIYVEFPLTE
                     EGAAALAGRVPGLAGRPISVLIWTTTPWTIPANLAIAFHPDFAYAAYPVGDTTVIVAE
                     ALASDVSRAVGRTFGEPVARFKGEVLEGIRFSHPLYARESVGVLASYVTLDQGTGAVH
                     TAPGHGADDFITGLRYGLEAYAPVDPGGRFTEDVELFAGQAVFEANAAIADALRARGR
                     LWKVEALTHSYPHCWRCHNPVIFLATSQWFISMDAGDLRAKALEAVGRVRWVPAWGEE
                     RIRGMVANRPDWCISRQRSWGVPIPALACTSCQESILTAALARRAADVFEEHGADSWY
                     ERPIEEFVPEGLKCPACGGTSFERGLDILDVWFDSGSSHEAVVAKRPGLSWPADLYLE
                     GTDQYRGWF"
     gene            complement(1865..2722)
                     /locus_tag="PKK95_06165"
     CDS             complement(1865..2722)
                     /locus_tag="PKK95_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17830.1"
                     /translation="MSKYLVAGLLALSFAFPVTSASAQERVTAVLKSGERVTGELVDL
                     GGRDYTFRVNGRERRIRIADMAMIDFPGTAPNVSESELARAGQGNHVIVLRNGQTLNG
                     RLVDISESSRPLVLHLEVNGERRRMTSQQVARIYMDQAGATATSGGGEQALGPGSRSI
                     QVSATRAWTPTGITVRKGDIVRFSSSGEVRVGPNASDTSNVNGRPGAYAVRATAPRVL
                     LGALIGRVGNGDPFGIGGQESVPMPDSGELFLGVNDANFGDNSGEFRVIVGVGAARTG
                     ERAIPRRRR"
     gene            2856..3590
                     /locus_tag="PKK95_06170"
     CDS             2856..3590
                     /locus_tag="PKK95_06170"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC17831.1"
                     /translation="MARPTRAVFFDVDFTLIYPGPFFQAHGYRDFCARHGIHDCDPQA
                     YDAAVASASALLDESDDLAYDPAFFLRYIRHLIERMGGSGPNLDRCAAEVYREWAACH
                     HFKLYDDVLPAFRELRVRGLRLGLISNTHRSLGDFASHFELDGLVSGWVSSCEHGFNK
                     PHPSIFRTALNLLGVEAGEAVMVGDSYRHDIEGARSVGMRGILLRRSAPPAGPLRPCA
                     VTLPSDVVVVRSLCEVAPVLSSASRP"
     gene            3670..4011
                     /locus_tag="PKK95_06175"
     CDS             3670..4011
                     /locus_tag="PKK95_06175"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOC17832.1"
                     /translation="MSCLFCAILAHEVPGQILYEDDTLAVLQDVRPQAPTHVLVIPRR
                     HIATLNDLRPEDDALVGAMFRRAAAVAAERGIAAAGYRTVFNCNRQAGQSVFHIHLHV
                     IGGRPLGWPPG"
     gene            complement(4041..4916)
                     /gene="prmC"
                     /locus_tag="PKK95_06180"
     CDS             complement(4041..4916)
                     /gene="prmC"
                     /locus_tag="PKK95_06180"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOC17833.1"
                     /translation="MAASPDSPATPRQLAALGRARLAGAGIEPGEAAMDAELLLREAL
                     GGWDRARFLAHQDDPATERTGPAFEALIERRARREPISLVLGRREFWGLEFRVTRDVL
                     TPRPETELVVEEALACAATLPRRASVADVGTGSGCLAVSLAIELPAASVLATDVSAAA
                     LEVARENAARHGVAGRITFERASLLGGAAGLALVVSNPPYIPTRDIETLPPEVRLHEP
                     RAALDGGPDGLDVVRALLAAALEALMPGGWLVFEFGDGQAQAVQAAVASSPLEMVRIR
                     ADLQGIPRTAAVRRP"
     gene            complement(4922..5989)
                     /gene="prfA"
                     /locus_tag="PKK95_06185"
     CDS             complement(4922..5989)
                     /gene="prfA"
                     /locus_tag="PKK95_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOC17834.1"
                     /translation="MIDKLNSIESRYEELLARIADPAVQNDPAEYRTHAKALAEIEPL
                     VERFREFKSVLTQIAQARELASDPDMRELAQEELQSLEARREALEQEIKVLLLPRDPN
                     DEKNVVLEIRAGTGGEEAALFAAELFRMYSRYAESNGWRVEVLSTSESGVGGMKEVIA
                     LVEGRQVYSRLKYESGVHRVQRVPATEASGRIHTSTATVAVLPEAEEVDIAIDPKDLR
                     IDTFCSSGPGGQSVNTTYSAVRITHIPTGIVVSQQDEKSQIKNRAKAMKVLRSRLYEM
                     ELRRQQEAIAKDRRSQVGTGERSEKIRTYNFPQSRITDHRINFTTHQIVNVMNGALGE
                     LIEQLSTHFQTEKLKEVTAVS"
     gene            complement(6020..6253)
                     /gene="moaD"
                     /locus_tag="PKK95_06190"
     CDS             complement(6020..6253)
                     /gene="moaD"
                     /locus_tag="PKK95_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HOC17835.1"
                     /translation="MRLFARLRELAGSGELRREVPAGATVAALWEALADEFPSLRPHS
                     TSVSAAVNQEFARRTKELADGDEVAFLPPVSGG"
     gene            complement(6256..6744)
                     /locus_tag="PKK95_06195"
     CDS             complement(6256..6744)
                     /locus_tag="PKK95_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOC17836.1"
                     /translation="MSDRQAAIVSSPLDAAEVVRLVTSDEPAARAGAIVTFIGTVRGD
                     RDGRRVAWLDYEAYDEMAVRVFRGLLEEAREHWPAARAAVHHRTGRVLPGEASVVIAA
                     SAPHRAEAFAACRYLIERIKQVAPVWKRECFEDGASWVEGAVADPADPEARGRAYERA
                     CR"
     gene            complement(6818..7447)
                     /locus_tag="PKK95_06200"
     CDS             complement(6818..7447)
                     /locus_tag="PKK95_06200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC17837.1"
                     /translation="MAVLAGLPAAVTGQNQQRHGRLFPPEDLGLLESPDRDVWQRPER
                     IMDSLGIADGSKVADVGAGGGWFTVRLARRVGPNGIVYAQDVQPQMLEAIERRVAKEN
                     LRNVVTIHGTPTDPNLPIEALDAVLIVETFHEIQSGNPRLFLENVRRSLKEGGRVGIV
                     DYKRGGGGPGPDGARPDPAAVEAAASAAGLRLLKRETFLPFQFFLVFAR"
     gene            complement(7486..8883)
                     /locus_tag="PKK95_06205"
     CDS             complement(7486..8883)
                     /locus_tag="PKK95_06205"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOC17838.1"
                     /translation="MNYRTERDPLGERQVPADALYGIQTQRAVDNFPISGLRAHHDLV
                     TATVQVKKAAARANAALGRLAADTARAIVTAADEVLAGAWRDQFVVDVYQAGAGTSHN
                     MNANEVLANRAAEILGGPRGTYHVVHPNDHVNMGQSTNDVFPAATRLALLLGLEPLLG
                     AAARLAGSLGRKASAFDGILKVGRTHLQDAVPITLGQEFSGFRASVEAGATGVAASAD
                     GLRELNLGATAVGTGLNAGDEYVRLVIENLREQTGLDVRPAANLFRVTQSMGDVLAYS
                     GAVRRLAVEIGKIASDLRLLGMGPRAGLGEIQLPAVQPGSSIMPGKVNPSVPEMVNQV
                     CYQVIGCDTTIAISAEAGQLELNVMMPVIAWNALHASTILRQAMRSLAERTVDGLTAD
                     PARCRELLERSTAVATALSPYIGYAATAEIAKESVRTGRPIRDLVLERGLIDRERLDR
                     ILSPEEMTRPGVPGQ"
     gene            9024..10541
                     /locus_tag="PKK95_06210"
     CDS             9024..10541
                     /locus_tag="PKK95_06210"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOC17839.1"
                     /translation="MLPAFLGSMLELIVKTSTDLAPDVRAAMKTALEQEPAGTQSAQA
                     LTIIAQNIDQAVEGEGAICQDTGMPTFEIHAPIGADQAWMARQIQDAIAEATRRGKLR
                     PNSVDSITGKNSGNNLGPGTPVIHFTQWDKDEIEVRLLLKGGGCENTNIQYSVPIELT
                     HLGRADRNLDGVRKCILHAVWQAQGKGCSPGAVGVCIGGDRATGYAHAKQQLFRPLDD
                     VNPDPALADLEASIMKTVNVLGIGTMGFGGRTSLIGCKVGVLNRLPASFFVSVAYDCW
                     AFRRLGVVLDPRDGSIAHWLYRDPAHPSLPMLDQAGFQRSGREAVLSPPISEEQVRSL
                     KVGDVVLLNGTMYTGRDSVHSYLMKNDAPVDLRGGVLYHCGPVVLKDGEAWKMMAAGP
                     TTSIREEPYQADVIRRFGIRAVIGKGGMGAKTLAALEDSGAVYLSAIGGAAQFYARCV
                     ERVSGVSLLDFGVPEAMWHLQVRDFPCIVTMDSHGNSLHKDVEAASAGKLATLAS"
     gene            10626..10964
                     /gene="erpA"
                     /locus_tag="PKK95_06215"
     CDS             10626..10964
                     /gene="erpA"
                     /locus_tag="PKK95_06215"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOC17840.1"
                     /translation="MATINVSPVAATKITELLAEEQKQECGLRVFVQGGGCSGFQYGL
                     MIEETPGTTDQVIESNGVRLYVDPISVRYLNGAEVDFVDNVAGGGFTIRNPNAVTTCG
                     CGQSFTVDEN"
     gene            11027..>11828
                     /locus_tag="PKK95_06220"
     CDS             11027..>11828
                     /locus_tag="PKK95_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOC17841.1"
                     /translation="MTHTLPTPDLDRLKPAGTRRSGKREFIVNVFLRHEGHLSADDLV
                     ELIRREDSRISRATVYRALQWMEEAGIARKVDFGEGRFRFEHSYRHPRHFHLICKSCG
                     RSFEFLSSDIEALIEEVASARAFSAHQSVVQIYGACEACRTGRTEAAEFVPAEVLFAR
                     DALRIAIATERSGLEFYSRAAAVTRNRRGRAVFERLAREEREHLEKLEARYQRLLAQD
                     PQLESRPTFLFFKGAANGLFAAGAEELARGLDDRKALMLAVRCERTSHR"
BASE COUNT         1704 a         4291 c         4037 g         1796 t
ORIGIN      
        1 ggaaccagcc gcggtactga tcggtccctt ccaggtacag gtcggcgggc caggagagcc
       61 ccgggcgctt cgcgaccacg gcctcgtggc tcgaccccga gtcgaaccac acgtccagga
      121 tgtcgagccc ccgctcgaac gaggttcctc cgcatgccgg gcacttcagt ccctcgggga
      181 cgaactcctc gattggccgc tcgtaccacg aatcggcccc gtgctcctcg aacacgtcgg
      241 ccgcgcggcg cgcgagggcc gccgtgagaa tcgattcctg gcagctggtg caggccagtg
      301 ccgggatcgg gacaccccag gagcgctgcc tcgagatgca ccagtcggga cgattggcca
      361 ccatcccgcg aatccgctcc tcaccccagg cgggaaccca ccgcacgcgc cccacggcct
      421 cgagcgcctt cgccctcagg tcgccggcgt ccatgctgat gaaccactgc gaggtcgcca
      481 ggaagatcac cgggttgtgg cagcgccagc agtgcgggta cgagtgcgtc agggcctcga
      541 ccttccatag ccgcccccga gcccgcagcg cgtccgcgat ggccgcgttc gcctcgaaga
      601 ccgcctgccc agcgaacagc tcgacgtcct cggtgaagcg gccgccgggg tccaccggcg
      661 cgtaagcctc gagcccatac cgcagcccgg tgatgaagtc gtcggcgccg tgccccggcg
      721 cggtgtggac ggcccccgtg ccctggtcca gcgtgacgta gctggcgagc acgcccaccg
      781 attcgcgtgc gtacaacgga tgcgagaagc ggattccctc gagcacctcg cccttgaagc
      841 gcgcgaccgg ctcgccgaag gtccgcccca cggcgcgcga gacgtcgctg gccaacgcct
      901 ctgcgacgat caccgtcgtg tcgccgacgg ggtaggcggc gtacgcgaag tcggggtgga
      961 aggcaatcgc caggttcgcc gggatcgtcc agggcgtagt cgtccagatg aggaccgaga
     1021 tcggccgtcc cgccagcccg ggcacgcgcc cggccagcgc cgcggcgcct tcctcggtca
     1081 gggggaactc gacgtagatc gacggggacg tgtggtcctc gtactcgacc tcggcctcgg
     1141 ccagcgccgt gcggtcgacg atgcaccagt ggaccggctt cttgcccttg tagacccgcc
     1201 cctgctcgac gaaccgcgcc agcgaccgca cgatggtcgc ctggtagcca tacgccatcg
     1261 tcaggtaggg cgagtcccag tcgccggcga cgccgagacg cttgaacccg gcccgctgga
     1321 tgtcgacgaa gcgcgtcgcg tactcgcggc acgcccggcg cacctcgacg gtggtcatcg
     1381 cgcgcctgcg ctcgcccaac tcgcggtcca cgttgcgctc gatcggaagg ccgtggcagt
     1441 cccatccggg gacgtacggc gcgtcgaacc ccgccatgct gcgcgaccgc accacgaagt
     1501 ccttgaggat cttgttgagc gccgtgccga ggtgcagggg gccgttcgcg tagggcggcc
     1561 cgtcgtgcag gatgaaggtg ggagccccct tccgcctgcg gcggagctgc tcgtacagcc
     1621 ctgtcgcctc ccagcgggcg atggcggcgg gctcggcgga ctgcaggttc gccttcatgg
     1681 ggaacccggt ccgaggcagg ttcaacgtgt ccttccagtc ggccataggg cgtgagtgtc
     1741 ctgatcccgg gaaaacggga aaagatcatt gtactgcagg gggaagccgc gggcggcgcg
     1801 aggtgggagg cgaccgcggg cgggactcgc ccgcccgcgg cgccgcgaaa accgggacga
     1861 ccctctaccg gcggcgccgg gggatggcgc gctcgccggt ccgcgccgcg ccgacgccga
     1921 cgatcacgcg gaactcgccg gagttgtcgc cgaagttggc gtcgttcacg cccaggaaca
     1981 gctcgcccga gtcgggcatc ggcaccgact cctggccgcc gatcccgaat gggtcaccgt
     2041 tgccgacgcg cccgatgagc gcgccgagca gcacgcgggg cgcggtggcg cgaaccgcgt
     2101 aggcgcccgg cctgccgttc acgttggacg tgtcgctggc gttgggaccc actcgaacct
     2161 cgccactcga agagaaccgg acgatgtcgc ccttgcgtac cgtgatcccg gtcggcgtcc
     2221 acgccctggt ggccgagacc tggatcgagc gagacccggg gccgagagcc tgttcgccgc
     2281 cgcccgaggt ggccgtcgcg cccgcctgat ccatgtagat gcgcgcgacc tgctgcgagg
     2341 tcatccgccg ccgttcgccg ttcacctcga gatgcaggac gagcggccgc gacgactcac
     2401 tgatgtccac cagcctgccg ttcagcgtct gcccgttgcg cagcacgatg acgtggttcc
     2461 cctgcccggc acgcgccagc tcggactcgc tgacgttcgg cgccgtgccc gggaagtcga
     2521 tcatcgccat gtccgcgatc cggatccggc gctccctgcc gttgacccga aacgtgtagt
     2581 cacggccgcc gaggtccacc agttcgccgg tcacccgctc gcccgacttc aggacggccg
     2641 tcacccgctc ctgcgccgag gcgctcgtga ccgggaacgc gaagctgagg gccagcaggc
     2701 cagcaacgag gtatttgctc attgattcct ccagttctct gcccggcttt cggctgcaaa
     2761 gctcctgcca ggcttcgccg ccggtttcgc cccgactttc gcacgcaacg ggcgtcctgt
     2821 cccgaatcat actcgcttgc ggtacgatcc ggacaatggc gaggccgacc cgagccgtct
     2881 tcttcgacgt ggacttcacg ctcatctacc cggggccctt cttccaggcc cacggctacc
     2941 gcgacttctg cgcccgccac ggcatccacg actgcgatcc gcaggcgtac gatgccgccg
     3001 tcgcgagcgc gtcggccctt ctcgacgagt cggacgatct cgcctacgac ccggcgttct
     3061 tcctgcgcta catccgccac ctgatcgagc ggatgggcgg gtcgggcccc aacctcgatc
     3121 gctgcgccgc ggaggtctat cgcgagtggg ccgcctgcca ccacttcaag ctgtacgacg
     3181 acgtcctccc ggcgttccgc gagctgcggg tgagaggcct gcggctcggc ctcatctcga
     3241 acacccaccg ctcgctcggc gatttcgcca gccacttcga gttggatggg ctggtgtcgg
     3301 gttgggtctc ctcgtgcgag cacgggttca acaagccgca cccgagcatc ttcaggacgg
     3361 cactgaacct gctgggagtc gaggccggcg aggcggtgat ggtgggcgac agctatcggc
     3421 acgacatcga gggcgcccgc tcggtcggca tgcgcggcat cctgctgcgg cgttccgccc
     3481 cgccagccgg tccgcttcgg ccgtgcgccg tcacgctgcc gtccgacgtc gtcgtggtgc
     3541 gctcgctttg cgaggtggcg ccggtgctgt cgtctgcttc gcggccttga gcccgccttc
     3601 gccacgcgct cgctacgctc gcggctacgg cggggcaagc ccttgagtcc tgagccctga
     3661 gtcttcctta tgtcctgtct cttctgcgcc atcctcgccc acgaagttcc cggtcagatc
     3721 ctgtacgaag acgacaccct cgccgtcctg caggacgtca ggccgcaggc tccgacccac
     3781 gtgctggtga tcccgcgacg gcacatcgcc acgctcaacg atctgcgtcc cgaggacgac
     3841 gcgctggtgg gggcgatgtt ccggcgggcg gcagccgtcg cggccgaacg cggtatcgcc
     3901 gcggcgggtt accgcacggt cttcaactgc aaccggcagg cgggtcagtc ggtgttccac
     3961 atccacctgc acgtgatcgg cggccgcccc ctcggctggc cgccggggta gtagcgccgg
     4021 cctccgtccc gcggcttgga ctaaggccgc cgcacggccg cggtacgagg gattccctgg
     4081 aggtcggcgc ggatcctcac catctccagc gggctcgacg ccaccgcggc ctgcacagcc
     4141 tgcgcctgtc catcgccgaa ctcgaacacc agccagccgc ccggcatgag cgcctccaag
     4201 gcagccgcca gcagcgcccg cacgacgtcg agtccgtcag ggccgccgtc gagggccgct
     4261 cgcggctcgt gcaggcgaac ctcgggcggc agggtctcga tgtcgcgtgt gggtatgtac
     4321 ggcgggttcg acacgacgag ggcgaggcca gccgcaccgc cgagcaacga ggcgcgctcg
     4381 aacgtgatgc gcccggcgac gccgtggcgg gccgcgttct cgcgggccac ctcgagtgcg
     4441 gccgccgaga cgtcggtcgc gagcacgctc gcggccggca gctcgatggc gagggagact
     4501 gcgaggcagc ccgaaccggt gccgacgtcg gcgaccgagg cgcgacgcgg gagcgtggcg
     4561 gcgcaggcca gggcttcctc gaccaccagc tcggtctcgg ggcgcggcgt gagcacgtcg
     4621 cgggtcaccc ggaactcgag gccccagaac tcgcggcggc cgaggaccag ggaaatcggc
     4681 tcgcggcgtg cgcgccgctc gatcagcgcc tcgaaggccg gcccggttct ctcggtggcc
     4741 gggtcgtcct ggtgggccag gaaccgggcg cgatcccagc cgccgagggc ctccctgagc
     4801 agcagctcgg cgtccatcgc cgcctcgccc ggctcgatgc ccgcgcccgc caggcgcgcg
     4861 cggcccagcg cggcgagctg ccgcggcgtc gccggcgagt caggtgaggc ggccatctgc
     4921 gtcaggagac cgcggtgact tccttcagct tctcagtctg gaagtgcgtg gagagctgct
     4981 cgatcagctc gccgagcgcc ccgttcatca cgttgacgat ctggtgggtg gtgaagttga
     5041 tgcggtgatc ggtgatgcgg ctctgcggga agttgtaggt acggatcttc tccgatcgct
     5101 cgcccgtgcc gacctggctg cgacggtcct tggcgatggc ctcctgctgc cgccgcagct
     5161 ccatctcgta cagccgcgac cgcagcacct tcatcgcctt cgcgcggttc ttgatctgcg
     5221 acttctcgtc ctgctgcgag acgacgatgc cggtggggat gtgagtgatg cggacggcgg
     5281 agtacgtcgt gttgacgctc tgcccgcccg ggccgctcga gcagaaggtg tcgatgcgga
     5341 ggtccttcgg atcgatcgcg atgtccacct cctcggcctc gggcaggacc gccaccgtgg
     5401 cggtggaggt gtggatgcgg ccgctcgcct cggtggcggg cacgcgctgc acccggtgca
     5461 cgccgctctc gtacttcagg cggctgtaga cctggcgccc ctcgaccagg gcgatcactt
     5521 ccttcatccc cccgacgccc gactcgctcg tggacagcac ctcgacccgc cagccgttgg
     5581 actcggcgta ccgcgagtac atccggaaca gttcggcggc aaacagcgcc gcctcctcgc
     5641 cgcccgtgcc cgcgcggatc tcgagcacga cgttcttctc atcgttcggg tcacggggca
     5701 gcaggaggac cttgatctcc tgctcgagcg cctctcgccg cgcctcgagc gactgcagct
     5761 cctcctgtgc cagctcgcgc atgtcggggt cactggcgag ctccctcgcc tgggcgatct
     5821 gggtgagcac cgacttgaac tcgcggaacc gctcgaccag gggttcgatc tcggcgaggg
     5881 ccttggcgtg cgtgcggtac tcggccggat cgttctgcac ggccgggtcg gcaatccgcg
     5941 ccagcagttc ctcgtaccgc gactcgatgg agttcagctt gtctatcatg accgtcccgt
     6001 agggtgctga aagaagaact cagccgcccg agaccggcgg caggaacgcc acctcgtcgc
     6061 cgtcggcgag ctccttcgtc cggcgcgcga actcctggtt cacggcggcc gaaaccgatg
     6121 tcgagtgcgg gcgcaggctg gggaactcgt cggccagcgc ctcccagagg gcggctaccg
     6181 tggccccggc gggcacctcg cggcgaagct cgcccgatcc ggccagctcg cgcaaccgag
     6241 cgaaaagtcg caccctcacc tgcatgcgcg ttcgtacgcc cgcccccggg cctcggggtc
     6301 ggcgggatcc gccaccgccc cttccaccca ggacgcccca tcctcgaagc actcccgctt
     6361 ccacacggga gcgacctgct tgatgcgctc gatgaggtag cggcaggcag cgaacgcctc
     6421 ggcccggtgg ggtgccgagg ccgcgatgac cacgctcgcc tcgcccggca gcacgcgtcc
     6481 ggtcctgtgg tgcaccgccg cgcgcgccgc cggccagtgc tcgcgggcct cctcgaggag
     6541 acccctgaac accctgaccg ccatctcgtc gtacgcctcg tagtcgagcc aggcgaccct
     6601 tcggccgtcc cggtcgccgc gcaccgtgcc gatgaatgtg acgatcgctc ccgcgcgggc
     6661 cgcgggctcg tccgaggtca ccaggcggac cacctcggcc gcgtcgagcg gagacgacac
     6721 gatggccgcc tgccgatcgg acatccagta agtatacctt tcgattgcgc tgccggctgg
     6781 cgacgcggct gggaccgtcg cggtggggcg aggcgcctca ccgcgcgaac acgaggaaga
     6841 actggaacgg caggaaggtc tcgcgcttca gcaggcgcag gcccgccgcg cttgcggccg
     6901 cctcgacggc cgccgggtct ggccgtgcgc cgtctgggcc gggtcctccg cctccccgct
     6961 tgtagtccac gatccccacg cggccccctt ccttcagcga ccgccgcacg ttctcgagga
     7021 agagccgcgg gttcccgctc tggatctcgt ggaacgtctc cacgatcagc acggcgtcga
     7081 gcgcctcgat cggcaggttc gggtcggtcg gcgtcccgtg gatcgttacg acgttgcgca
     7141 ggttctcctt ggccacccgc cgctcgatcg cttcgagcat ctgcggctgc acgtcctgcg
     7201 cgtagacgat cccgttcggc cccacccggc gcgccagcct gaccgtgaac cacccgccgc
     7261 ccgcgccgac gtcggcgacc ttcgacccgt ccgcgatgcc gagcgagtcc atgatccgct
     7321 cgggccgctg ccagacgtcc cgatcggggc tttcgagcag gcccaggtcc tcgggcggga
     7381 acaggcggcc gtgccgctgc tggttctgcc cggtgacggc ggccggcagg ccggccagga
     7441 ccgccatggc tatcgccgcc ccgattgccg cgcgcgccct ggctctcact gtcccggcac
     7501 ccccggcctg gtcatctcct ccggcgagag gatgcgatcc aaccgctcgc gatcgatcag
     7561 gccgcgctcg aggacgaggt cccggatggg acgccccgtc cgcaccgact ctttcgcgat
     7621 ctccgccgtg gcggcgtacc cgatgtaggg gctgagcgcg gtggcgacgg cggtgctccg
     7681 ctccagcagc tcccggcagc gcgccgggtc ggcggtcagg ccgtccaccg tgcgctcggc
     7741 cagcgacctc atcgcctggc gcaggatggt cgaggcgtgg agggcgttcc aggcaatcac
     7801 cggcatcatc acgttcagct cgagctgacc ggcctcggcg gagatcgcga tcgtggtgtc
     7861 gcacccgatc acctggtagc agacctggtt caccatctcg ggcaccgagg ggttcacctt
     7921 gcccggcatg atcgaggagc ccggctgcac cgcaggcagc tggatctccc caagccccgc
     7981 acgagggccc atccccagga gccgcaagtc gctcgcgatc ttgccgatct ccacggccag
     8041 gcgccgcacc gctcccgagt acgccagcac gtcgcccatg ctctgcgtga cgcgaaacag
     8101 gttggcggcc gggcgcacgt ccaggcccgt ctgctcgcgc aggttctcga tcacgagccg
     8161 cacgtactcg tcgcctgcgt tcagcccggt gcccacggcg gtcgcgccga ggttcagctc
     8221 gcgcagcccg tcggccgagg cggcaacccc cgtggcgccc gcctcgaccg aggcgcggaa
     8281 ccccgagaac tcctggccga gggtgatggg caccgcgtcc tggaggtgcg tgcgccccac
     8341 cttgaggatc ccgtcgaacg ccgacgcctt gcggccgagc gagccggcga ggcgcgcggc
     8401 cgcccccagc agcggctcga ggccgagcag cagcgccagg cgcgtggccg ccgggaagac
     8461 gtcgttggtc gactggccca tattgacgtg gtcgttgggg tgcaccacgt ggtacgtgcc
     8521 gcgaggcccg cccaggatct cagcggcgcg gttggccagc acctcgttcg cgttcatgtt
     8581 gtgcgaggtg ccggccccgg cctggtacac gtccacgacg aactggtcgc gccaggcccc
     8641 ggccagcacc tcgtcggctg ccgtcacgat cgcgcgcgcc gtgtcggcgg ccagccggcc
     8701 cagcgcggcg tttgcccgcg ccgctgcctt cttcacctgc acggtggcgg tcaccaggtc
     8761 gtggtgggcg cgcaggccgc tgatcggaaa gttgtccacg gcccgctggg tctggatccc
     8821 gtagagcgcg tcggcgggga cctgacgctc gccgaggggg tcgcgttcgg tgcggtagtt
     8881 caaggcctgc cacctcgcgg ggcggggggc cgcacccgct tcacagcccc gaaggcctgt
     8941 gccggtctcc ggtgcggagg ccggcgcctg cgaagccggc acacgaagac ccgcaccgtt
     9001 cctttggcga tatactctgc gccatgttac cagcattcct cggcagcatg ctcgagctca
     9061 tcgtcaagac gtccaccgac ctggcgcccg acgtgcgcgc ggcgatgaag accgcgctcg
     9121 agcaggagcc ggccgggacc cagtcggcgc aggcgctcac gatcatcgcg cagaacatcg
     9181 accaggcggt ggagggcgag ggggcgatct gccaggacac cggcatgccg accttcgaga
     9241 tccacgcgcc gatcggtgcc gaccaggcct ggatggctcg ccagatccag gacgcgatcg
     9301 cggaggcgac tcgccggggg aagctgcgcc cgaattccgt cgactcgatc acggggaaga
     9361 actctggcaa caacctcggc ccgggcacgc cggtcatcca cttcacgcag tgggacaagg
     9421 acgagatcga ggtcaggctg ctgctgaagg gaggcggctg cgagaacacc aacatccagt
     9481 actcggtccc gatcgaactg acccacctcg ggcgcgccga ccgcaacctc gacggcgtgc
     9541 gcaagtgcat cctgcacgcg gtctggcagg cgcagggcaa gggctgcagc ccgggagccg
     9601 tcggggtctg catcggcggc gaccgcgcca ccggctacgc ccatgccaag cagcagctgt
     9661 tccggcccct cgacgacgtg aaccccgacc cggcgctcgc cgatctcgaa gcgtcgatca
     9721 tgaagaccgt gaacgtcctc ggcatcggca cgatgggctt cggggggagg acgtcactca
     9781 tcgggtgcaa ggtcggcgtg ctcaaccgcc tgccggcgag cttcttcgtg tcggttgcgt
     9841 acgactgctg ggccttccgc cgcctcggcg tggtgctcga cccgcgtgac ggctcgattg
     9901 cccactggct ctaccgcgac ccggcgcacc cctcgctgcc gatgctcgac caggccggct
     9961 tccagcgcag cggccgcgag gccgtgctgt ccccgccaat ctcggaagaa caggttcgct
    10021 cgctgaaggt cggcgacgtc gtgctgctga acgggacgat gtacaccggc cgcgactccg
    10081 tccacagcta cctgatgaag aacgatgccc cggtcgactt gcggggcggc gtcctctacc
    10141 actgcgggcc ggtggtgctg aaggacggcg aggcgtggaa gatgatggct gccggcccaa
    10201 ccaccagcat ccgcgaagag ccctaccagg ccgacgtcat caggaggttc ggcatccggg
    10261 cggtcatcgg aaaggggggc atgggggcga agacgctggc cgccctcgag gactcgggcg
    10321 cggtctacct gagcgcgatt ggaggggcgg cccagttcta cgcccgctgc gtggagcggg
    10381 tgagcggggt gtcgctgctc gacttcggtg tgcccgaggc gatgtggcac ctgcaggttc
    10441 gcgatttccc ctgcatcgtg accatggact cgcacgggaa cagcctgcac aaggacgtcg
    10501 aggcggcctc ggccgggaag ctggccaccc tggcgtcctg agcccgcctc cgtcaaacgc
    10561 tcgcgacgct cgcggctaca cccaatacgc tatacttgtg ggggaaggag acccccgaaa
    10621 aagacatggc taccatcaac gtgtccccgg ttgcggcgac caagatcacc gagctgctcg
    10681 ccgaggagca gaagcaggag tgcggcctgc gcgtcttcgt gcagggcggc ggctgctcgg
    10741 gcttccagta cgggctcatg atcgaggaga cccccggcac gaccgatcag gtcatcgagt
    10801 cgaacggcgt ccggctctac gtcgacccca tcagcgttcg ctacctgaat ggggccgagg
    10861 tggatttcgt ggacaacgtg gcgggcggcg gcttcaccat ccgcaacccc aacgccgtga
    10921 cgacctgcgg gtgcggccag tcgttcacgg tcgacgagaa ctgacgctcg atcgggcagt
    10981 tgcggccgca ccgggtcgga cgacagccct gccgggcgca actgccatga cccacaccct
    11041 tcccaccccg gacctggacc ggctcaaacc cgccgggacc cgccgctcgg gcaagcgcga
    11101 gttcatcgtc aacgtgttcc tgcgccacga ggggcacctg agcgccgacg acctggtgga
    11161 gctgatccgc cgcgaggaca gccggatcag ccgggccacc gtctaccgcg cgctgcagtg
    11221 gatggaggag gccgggatcg cccggaaggt ggacttcggc gagggccgct tccgtttcga
    11281 gcactcctat cggcaccccc gccacttcca cctgatctgc aagtcgtgcg gccggtcgtt
    11341 cgagttcctc agctccgaca tcgaggcgct gatcgaggag gtcgcctcgg cgagggcttt
    11401 ctcggcccat cagagcgtgg tgcagatcta cggcgcctgc gaggcgtgcc ggacgggccg
    11461 cacggaggcc gccgagttcg tgccggccga ggtgctcttc gcgcgcgacg ccctgcgcat
    11521 cgcgattgcg accgagcgca gcggcctcga gttctactcg cgcgccgccg cggtgacgcg
    11581 caaccgccgg ggccgcgccg tgttcgagcg gctcgcccgc gaggagcgcg agcacctgga
    11641 gaagctcgag gcccgctacc agcggctgct cgcccaggac ccgcagctcg agtcgcgtcc
    11701 gaccttcctc ttcttcaagg gggcggccaa cgggctgttc gccgccggcg ccgaggagct
    11761 ggcccgcggc ctggacgacc ggaaggcgtt gatgctcgcc gtccggtgcg agcgcacctc
    11821 gcaccgct
//
LOCUS       DAOPQX010000079        11773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6288_length_11773_cov_4.424646, whole genome shotgun sequence.
ACCESSION   DAOPQX010000079 DAOPQX010000000
VERSION     DAOPQX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6288_length_11773_cov_4.424646"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..751
                     /gene="infC"
                     /locus_tag="PKK95_06225"
     CDS             152..751
                     /gene="infC"
                     /locus_tag="PKK95_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOC17842.1"
                     /translation="MAFDRSSPRRDDRTRINERIRVREVRVIDDAGQQLGIMAPQQAL
                     AIARQKGLDLVEVAPMAQPPVCRIMDFGKYQYQEQKRSREARKHQKVIEVKEIKFRPK
                     VDEHDYQFKKKHIERFLADGDKVKATIFFRGREMAHPEIGRRILERLIQELNEIAIAE
                     SMPRMEGNTMHTILTHRPAPGGGKPQAPKPPAGQPAPTR"
     gene            815..1012
                     /gene="rpmI"
                     /locus_tag="PKK95_06230"
     CDS             815..1012
                     /gene="rpmI"
                     /locus_tag="PKK95_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOC17843.1"
                     /translation="MPKLKTHRGAAKRFKKTGTGKFLRSQAFKRHQLSCKTTKEKRHL
                     TGTVVVSEQDTARIKRMLPYK"
     gene            1036..1410
                     /gene="rplT"
                     /locus_tag="PKK95_06235"
     CDS             1036..1410
                     /gene="rplT"
                     /locus_tag="PKK95_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOC17844.1"
                     /translation="MPRVKRGTVRRAKRKKLLGLAKGYYARKSKLYRSAKEAVDTALK
                     YAFVGRRNKKRDFRRLWIVRINAAAHEQGLRYSDFMRGLKLAGVALDRKMLADMAVKD
                     PASFARIAGQAKAALAAPQPQA"
     gene            1414..2508
                     /gene="pheS"
                     /locus_tag="PKK95_06240"
     CDS             1414..2508
                     /gene="pheS"
                     /locus_tag="PKK95_06240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOC17845.1"
                     /translation="MPDAAAIDRLRAEFEAALAEASSGEALRALRDRYLGRKGGVVAS
                     LMKSVAAAPAADRPLLGRLANELKREVETRIDQARAALEASRPVAGAVDVTLPGRPVA
                     LGRLHPVTIVRQQVESIFGRMGFEILEGPELEDDYHNFEALNMPPEHPARDMQDTLFL
                     ARPVPQNAPAIPGSRPSRGRPLERRPATLLRTHTSGMQIRYMEAHPPPVRVVAIGKVF
                     RRDNPDLTHTPMFHQFEGLLVDEGVTLADLKGTLTAFVREIFSPDTRVLFRPSFFPYT
                     EPSAEVFIGCVFCAGAGCPVCKRTGWLEILGSGMVHPAVLEAVGYDPERYTGFAFGTG
                     IERVAMLKYRVGDLRLFYENDLRFLEQFSL"
     gene            2505..4649
                     /gene="pheT"
                     /locus_tag="PKK95_06245"
     CDS             2505..4649
                     /gene="pheT"
                     /locus_tag="PKK95_06245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOC17846.1"
                     /translation="MKLLVSWLRDFVDVQVANTDLASLLSMRGFEVASIDPVAAAGAA
                     PLPDGAGLQPAGDAIIDFEITANRPDCLSVRGLAREAATALRLPYRAIHTAGSGEVEG
                     GAGASSAGGPNAPLSVHIDDPELCPRYAAAVAEVTVGPSPRWMQDRLEAAGVRPINNV
                     VDVTNYVLMELGQPLHAFDLERLGGRQLRARQARAGERLQTLDGEDRELAAGMLVIAD
                     ATTPQAVAGVMGGAPTEVWSGTRLIALESAYFKPASIRRTSKKLGLSTEASQRFERGG
                     DIGIPVAALERACALLEQIGAGTRRGPILDCYPAPRGPVAVPLRRTRIAALLGMTIPD
                     EEVEAILAGLGFELSRTADGWQAMVPSWRVDVAREADLVEEVGRHHGFDRIPSTFPAL
                     REAPPRPDPVIRRNQTLRVALVGAGFAEAIGYSFIERAAAAPFIDTLGAGTDPGAAGD
                     RLVPLAYPLSELFAVLRPSLLPGLLDAVVRNRRRETADVRLFEIGSCFSADDGERRRL
                     AFVWSGAGRPAHWASQDEPVTFFHVKGVVEQAAASLRLPPLVFEPAAATWLVPGRAAK
                     VSCDGRVLGLLGQIRPELAAAREMPGGDAVYAAELDLEALHALVPAADVRVEPLPRFP
                     SVVRDVSLVVDEGLPSGSIRATIRSAAPDTLVSIREFDRYQGAGIPEGRCSLSFRLTF
                     RSPDRTLTDAEVQQAMDRVVEAVKAVHQAVQR"
     gene            4665..4940
                     /gene="zapB"
                     /locus_tag="PKK95_06250"
     CDS             4665..4940
                     /gene="zapB"
                     /locus_tag="PKK95_06250"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HOC17847.1"
                     /translation="MARATVQADIEALDRLEQKVRTLVGTIGSLQAEQARLSEENRRL
                     EGELDSLRGRLQDAESASGELAALRQERDEVRSRVAGMLEALEGLDL"
     gene            4943..5236
                     /locus_tag="PKK95_06255"
     CDS             4943..5236
                     /locus_tag="PKK95_06255"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOC17848.1"
                     /translation="MAEPARVISVEILGQQYPIRSSLDARYVARLAAYVDEKIRTTAD
                     LTPATDTVRLAVLAALNIADECFHAREDDEVRREAVLERVARLEALIDEALGG"
     gene            5290..5473
                     /gene="ssrS"
                     /locus_tag="PKK95_06260"
     ncRNA           5290..5473
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKK95_06260"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5581..7158
                     /gene="rny"
                     /locus_tag="PKK95_06265"
     CDS             5581..7158
                     /gene="rny"
                     /locus_tag="PKK95_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOC17849.1"
                     /translation="MPMPGAVTLALSIVAAAIAVGVFAYWFASRKRIAAETIGRAEEQ
                     CRQLLREAEREGETRKKEALFEAKERAHQLVMEAERLAEDRRQQVGRLEQVLGKKEEK
                     LTERLAAAEQQERELRAREQSIRDRERATAAAAAKYEQLVADQQRELQRVAGLTTDEA
                     REILLKQIESDARRDAANTVKRIEAEAREQAAARAKQIVTEAIQRSAPEHATETTVSV
                     VDLPSDDLKGRIIGREGRNIRALEIATGVDLIVDDTPGAIILSGFDPYRREIAKQAIE
                     RLIADGRIHPARIEEVVEKVKAELEESVRREGEAAAFEMGIHDLHPEVHRLMGRLKFR
                     TSYGQNVLTHSKEVAYLAGMMARELDLDWHTTVRAAFLHDIGKAIDREMQGTHLEIGV
                     DFLRRHGETPAITAIVASHHMDTDWPCVEAMLVQAADAISAARPGARRDILESYVKRL
                     EKLESIADSFKGVAKSFALQAGREIRIMVESERITDEEAVWLSKDIARRIESELEYPG
                     QIKVTVIRETRAVEYAK"
     gene            7160..8182
                     /gene="thiL"
                     /locus_tag="PKK95_06270"
     CDS             7160..8182
                     /gene="thiL"
                     /locus_tag="PKK95_06270"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOC17850.1"
                     /translation="MPAPKVGDIGEREVIRRIRRRLPAPPDWLRTPIGDDAAVVRPPR
                     NRDEVVTTDAMVEGIHFDRSYVPPAAIGAKALAVNLSDLAAMGAEPRLALLSLILPAD
                     TTVSDLESLVDGMLGMAARHGVTLAGGNVARSPGPLVVDLTAIGCVKPRDVLLRRGAR
                     PGDGIYVSGAVGAARAGLLSLHGAHNVAGPAEGDGLDEVRARFLVPEPRVRLGLLLAR
                     NRVASACMDLSDGLADAVRGVAAAGRVGAIVEAEALPIPGAARRIFEVAGLDSIEAAL
                     EGGEDYELLFTVPRRRRRALTAVLALVPDVACTRIGHVTAERRIVLRREGVERALPAG
                     YSHFTG"
     gene            8179..8679
                     /locus_tag="PKK95_06275"
     CDS             8179..8679
                     /locus_tag="PKK95_06275"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOC17851.1"
                     /translation="MKGIRHSLWRKLVVTLIAGAGFVLLYQATIFDSRYAARQALLRE
                     DVADPGPGTRLRFLATAYCKGDITASGLPPRHGIAAADPDLLPVGSVVQIVRGAKGYE
                     GVYTIMDTGPVVRGRRLDLYIWSCNEALQFGRRSVQVVVLRLGWNPAASGPSLLDKLL
                     PWRTRQ"
     gene            8940..10139
                     /locus_tag="PKK95_06280"
     CDS             8940..10139
                     /locus_tag="PKK95_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC17852.1"
                     /translation="MRIAQVAPLFESVPPKLYGGTERVVSYLTEELVRLGHQVTLFAT
                     GDSRTSARLVPVWPQALRLDETCADQLAPHVLMIEQVARMADQFDVIHFHVAQLHLPL
                     ARRLHGVAHLTTLHGRLDLPELVPLFDEFSEIPVVSISDAQRQPLPRAAWVGTVHHGL
                     PAGLLPFSPGPGEYLAFLGRVSPEKGVGRAIAIARACGIPLRIAAKVDPADRVYFETE
                     IRPLLDDPLVEFIGEIGENEKGPFLGGARALLFPIDWPEPFGVVMIEALACGLPVVAF
                     EGGSVGEVISNGVNGFVVRSVEEAVHAVHRIGSIDRRACRADFERRFTASRMAADYLA
                     LYRAVRRAAAAFRLPRPALSPAVEAPVAAGEAPVPAGEYGGPANGRSGFTGGARRAND
                     LGESGHL"
     gene            10136..>11773
                     /locus_tag="PKK95_06285"
     CDS             10136..>11773
                     /locus_tag="PKK95_06285"
                     /inference="COORDINATES: protein motif:HMM:NF026092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HOC17853.1"
                     /translation="MTGPASKPGGVRLDEFAIISGGDRGGAPSRVLKQGDTFGVFDLH
                     GDAVPEGAREHGLYHEGTRFLSRLELRFGKSRPLLLGSRISDDNTVLAVDLTNPDVVK
                     DGRPLVRRGILHVFRARLLWDGHCLERIRLSNHALHPIELPLTLVFDADYADLFEVRG
                     THRASRGRLFAPSIAPDSALLRYEGLDGLERRTRLEWSAAADRVEDHGATFSVSLDPH
                     EVREIELSIAFELGAVTRPARSYDAALSSLKGEMAALQARECGVLTSNESFNRWLRRS
                     SADLRMMLTETPSGPYPYAGIPWYSTPFGRDGLLTAYEMLWAAPDVARGVLAFLAETQ
                     AKVVSDDQDAQPGKIIHEMRAGEMAALGEIPFGRYYGSVDATPLFVMLAHAYFGRTAD
                     RAFLDRLWPHLLAALEWMEEYGDLDGDLFIEYARRSPSGLLHQGWKDSNDSVFHEDGE
                     LAEGPIALCEVQAYAYGAWTGAAALAEVRGDLARARGWRDRAAALRERFDHAFWCEPL
                     GTYALALDGQKRPCRVRTSNAGHCLFSGLATRPRARSV"
BASE COUNT         1727 a         4251 c         4137 g         1658 t
ORIGIN      
        1 tgctcgtcgt cggggaccgc gaggccgaac agggcctggt ggccgtgcgc gcgcgcagtg
       61 gcggcgacca gggatcgcgc ccgctcgacg agttcgtcgc cgcggccctg gacgagatgg
      121 cgaggaaagg gaagtagggg aggaggctca tatcgctttc gatcgctcca gcccacgtcg
      181 tgacgaccgc acgcggatca acgagcgcat ccgggtgagg gaagtccgtg tgatcgatga
      241 cgccggccag cagctcggca tcatggcgcc gcagcaggcg ctggccatcg cccggcagaa
      301 ggggctcgac ctggtggaag tggcgccgat ggcgcagccg ccggtctgcc ggatcatgga
      361 cttcggcaag taccagtacc aggaacagaa acggtcccgg gaagcgcgga agcaccagaa
      421 ggtcatcgag gtcaaggaga tcaagttccg gccgaaggtg gacgagcacg actaccagtt
      481 caagaagaag cacatcgagc gcttcctggc ggacggcgac aaggtgaagg cgacgatctt
      541 cttccgcggg cgcgagatgg cccacccgga gatcgggcgc cgcatcctcg agcgcctcat
      601 ccaggagctg aacgagatcg caatcgccga gtcgatgccg aggatggagg gcaacaccat
      661 gcacaccatc ctcacccacc ggccggcccc gggcggcggc aagccgcagg cgcccaagcc
      721 gccggcaggt cagccggccc cgacgcggta gcggcgcggt gcacgaacag cggtagaggg
      781 ccttggccct gcgatggtca attgagagac gacgatgcca aaactgaaga cccaccgggg
      841 cgcggcgaag cgcttcaaga agacgggaac cgggaagttc ctgcggagcc aggcgttcaa
      901 gcgccaccag ctgagctgca agacgaccaa ggagaagcgc cacctgacgg gcaccgtggt
      961 ggtctccgag caggacaccg cccgcatcaa gcggatgctg ccatacaagt agggccagcg
     1021 taaggagaga ctgcgatgcc tcgtgtgaaa cgtgggacgg tgcgccgggc gaagcgcaag
     1081 aagctgctcg gactcgccaa gggctattac gcccgcaaga gcaagctgta ccgctcggcc
     1141 aaggaggcgg tcgacacggc gctgaagtac gcgttcgtcg gccgccgcaa caagaagcgc
     1201 gacttccgcc gcttgtggat cgtccgcatc aacgcggccg cgcacgagca ggggctccgg
     1261 tacagcgact tcatgcgcgg gctcaagctg gccggcgtgg ctctcgaccg gaagatgctg
     1321 gccgacatgg ccgtgaagga tcccgcctcc ttcgcccgga tcgcggggca ggccaaggcc
     1381 gcgctcgcgg cgccccagcc gcaggcgtag accgtgcccg acgccgccgc catcgaccgt
     1441 ctccgggcgg agttcgaagc cgccctcgcc gaggcgtcgt ccggcgaagc gctgcgggcg
     1501 ctccgcgacc ggtatctcgg acgcaagggc ggcgtggtgg cctcgctgat gaagtcggtg
     1561 gcggccgccc ccgccgccga tcggcctctc cttggccgcc tcgccaacga gctgaagcgc
     1621 gaggtcgaaa cgcgcatcga ccaggcgcgc gccgccctcg aggcgtcccg ccccgtcgcc
     1681 ggcgccgtcg acgtcacgct gcccggccgc cccgtcgccc tcggccgcct ccaccccgtc
     1741 accatcgtcc gccagcaggt cgagtcgatc ttcgggcgca tgggcttcga gatcctcgag
     1801 ggcccggaac tcgaagacga ctaccacaac ttcgaagcgc tcaacatgcc gcccgagcac
     1861 cccgcgcgcg acatgcagga cacgttgttc ctggcgcggc cggtgcccca gaacgccccg
     1921 gcaatcccgg gatcgcggcc gtcccgcggc cgccccctcg agcgccgccc ggccacgctg
     1981 ctgcgcacgc acacctcggg aatgcagatc cgctacatgg aggcgcaccc gcctcccgtg
     2041 cgggtcgtgg cgatcggcaa ggtgttccgg cgcgacaacc ccgacctcac gcacacgccg
     2101 atgttccacc agttcgaggg gctgctggtg gacgaagggg tgaccctggc cgacctgaag
     2161 ggcacgctga ccgcgttcgt ccgcgagatc ttctcgcccg acacgcgcgt gctgttccgg
     2221 cccagcttct tcccctacac ggagccgagc gccgaggtgt tcatcggctg cgtgttctgt
     2281 gcgggcgccg gctgcccggt gtgcaagcgc accggctggc tggagatcct tggcagtgga
     2341 atggtgcacc cggccgtcct cgaggccgtc ggctacgatc ccgagcgcta cacggggttc
     2401 gccttcggca ccgggatcga gcgggtcgcg atgctgaagt accgagtggg cgaccttcgc
     2461 ctgttctacg agaacgacct gcgcttcctg gagcagttct cgctttgaaa ctcctcgtct
     2521 cgtggctccg tgacttcgtc gacgtccagg ttgccaacac cgacctggcg tcgctgctct
     2581 cgatgcgtgg tttcgaggtg gcgtcgatcg acccggtcgc cgccgcgggc gccgcacccc
     2641 tgcccgacgg cgcggggctt cagcccgccg gcgacgcgat catcgacttc gagatcaccg
     2701 cgaaccgccc cgactgcctg agcgtccgcg gcctggcgcg cgaggcggcc accgcgctgc
     2761 gactccccta ccgcgcaatc cacacggcgg gttcgggcga ggtcgagggc ggcgctggcg
     2821 cgtcgagtgc cgggggcccg aatgccccgc tgtcggtgca catcgacgac cccgagctgt
     2881 gcccgcgcta cgccgcggcg gtcgcggagg tgacggtcgg cccgtcgccg cggtggatgc
     2941 aggaccggct cgaagccgcc ggtgtccgcc cgatcaacaa cgtggtcgac gtcaccaact
     3001 acgtgctgat ggagctgggg cagccgctgc acgccttcga cctcgagcgg ctgggaggcc
     3061 ggcagctgcg cgcgcggcag gcccgcgcgg gcgagcggct gcaaaccctc gacggcgagg
     3121 accgcgagct ggcggccggc atgctggtca tcgccgatgc caccaccccg caggcggtcg
     3181 ccggcgtgat gggcggcgcg ccgaccgagg tgtggtcggg caccaggctc atcgccctcg
     3241 agagcgcgta cttcaagccg gcatccattc ggcggaccag caagaagctc gggctcagca
     3301 ccgaggcgtc gcagcgcttc gagcgcggcg gcgacatcgg catccccgtg gcggccctcg
     3361 agcgcgcgtg cgcgctgctc gagcagatcg gcgccggcac gaggcgcggc ccgatcctcg
     3421 attgctaccc ggccccgcgc gggccggttg ccgtccccct gcgccgcacc cggatcgccg
     3481 cgctcctcgg gatgacaatc ccggacgagg aggtcgaagc gatcctcgcc ggcctgggct
     3541 tcgagctgtc gcgcacggcg gacggctggc aggcgatggt gcccagctgg cgcgtggacg
     3601 tcgcgcgcga agccgacctg gtcgaagagg tcggccgcca ccacggcttc gaccgcatcc
     3661 cgagcacctt cccggccctg cgcgaggctc cgccccgccc cgatccggtc atccggcgga
     3721 accagacgct gcgggtggcg ctggtcggcg ccggctttgc cgaggcaatc ggctacagct
     3781 tcatcgagcg ggccgccgcg gctcccttca tcgacacact gggcgcgggc accgatccag
     3841 gcgcggcggg cgaccgcctc gtgcccctcg cctacccgct ctccgagctg ttcgcggtgc
     3901 tgcggccctc cctgctcccc ggcctgctgg atgcggtcgt ccgcaaccgc cgccgcgaga
     3961 ctgccgacgt ccgcctgttc gagatcggct cctgcttctc ggccgacgac ggcgagcgcc
     4021 gccgcctggc gttcgtctgg agcggcgccg gccgcccggc ccactgggcg tcccaggacg
     4081 agccggtgac cttcttccac gtgaaggggg tcgtcgagca ggccgccgcg agcctgcggc
     4141 tgccgccgct ggtgttcgag cctgccgccg caacctggct ggttcccggg cgggcggcca
     4201 aggtgtcgtg cgacggccgc gtgctcggcc tgctcggcca gatccggccc gagctggccg
     4261 ccgcgcgcga gatgccgggc ggcgacgcgg tctacgccgc cgaactcgac ctcgaggcgc
     4321 tgcacgcgct cgtgcccgca gcggacgtcc gggtggagcc gctgccccgc ttcccctccg
     4381 tcgtgcgcga cgtgtcgctg gtcgtcgatg aaggcttgcc ttcgggaagc attcgcgcca
     4441 ctattcggtc tgcggcgccc gacaccctcg tttcgatccg cgagttcgac cggtaccagg
     4501 gggctggcat ccccgagggc cggtgcagcc tgtcgttccg gctcaccttc aggtcgccgg
     4561 atcgcacgct caccgacgcg gaggtgcagc aggcgatgga tcgcgtggtg gaggccgtca
     4621 aggcggtgca ccaggcggtg cagcggtaaa ggagtgaggt ccccgtggca cgagcaacgg
     4681 ttcaggcgga catcgaggcg ctcgatcggc tcgagcagaa ggttcggaca ctggtgggaa
     4741 ccatcggcag cctgcaggcc gagcaggcgc gcctgagcga ggaaaaccgg cgcctcgagg
     4801 gcgagctcga ctcgctgcgc ggccgcctgc aggacgccga gagcgcctcg ggcgagctgg
     4861 cggccctccg ccaggagcgc gacgaggtgc gctcgcgggt ggcgggcatg ctggaggcgc
     4921 tcgagggcct ggacctctga tcgtggccga acctgcccgc gtcatctcgg tggaaatcct
     4981 cgggcagcag tacccgatcc gcagttcgct cgacgcacgc tacgtggcca ggctggccgc
     5041 gtacgtcgac gagaagatcc ggacgacggc cgacctgacg cctgccaccg acacggtgcg
     5101 cctggccgtc ctggccgcgc tgaacatcgc cgacgagtgc ttccacgcgc gcgaggacga
     5161 cgaggtccgg cgcgaggcgg tgctcgagcg cgtggcccgc ctcgaagcgc tgatcgacga
     5221 ggcgcttggg ggctagtttt gtcccttgct tctgccaggg tgacggtcta agatcgtcgc
     5281 agacaattga gtaccctgct ttgcccgtga tggatgtgag gctcgcttga gccaacattt
     5341 ccaacaagcg gactgttagg tcgcgccgtg tgcatgcctc cgcgcgagga agcctgacgc
     5401 gcgatcaaca aggcggtccc acctgcgtta cgcaggttca aacgacctcc ccacacggca
     5461 aagcggggcc tttcttcttt ctctctctcc catcgaggtc ggttcgcggg ggggccgctc
     5521 gtccccgccg ttggtagccg ggcccccgcg tatcctgttt ttgaggggac tcgaaaaggg
     5581 atgccaatgc ccggtgccgt caccctcgcc ctctcgatcg tcgcggcggc catcgccgtc
     5641 ggcgtcttcg cctactggtt cgcctcacgc aagcgcatcg ccgccgagac catcggccgg
     5701 gccgaggagc agtgccgcca gttgctccgc gaggccgagc gcgaggggga gacccgcaag
     5761 aaggaagccc tcttcgaggc caaggagcgc gcccaccagc tggtgatgga ggccgagcgg
     5821 ctggccgagg accgccggca gcaggtcggc cggctcgagc aggtgctcgg caagaaggag
     5881 gagaagctca ccgagcggct ggccgcggcc gagcagcagg agcgcgagct gcgggcgcgc
     5941 gagcagagca tccgggaccg cgagcgagcc accgcggcgg cggccgccaa gtacgagcag
     6001 ctcgtggccg accagcagcg cgagctgcag cgggtcgccg gcctcaccac cgacgaggcc
     6061 cgcgagatcc tcctcaagca gatcgagtcg gacgcccgcc gcgatgccgc caacacggtc
     6121 aagcggatcg aggccgaggc ccgcgagcag gccgccgccc gcgccaagca gatcgtcacc
     6181 gaggcgatcc agcgcagcgc ccccgagcac gccaccgaga ccaccgtctc ggtggtcgac
     6241 ctgccgagcg acgacctgaa ggggcgcatc atcggccgcg aggggcgcaa catccgggcg
     6301 ctcgagattg ccaccggcgt cgacctcatc gtggacgaca cgcccggggc catcatcctc
     6361 tcgggcttcg acccctaccg ccgcgagatt gccaagcagg caatcgagcg gctgatcgcc
     6421 gacgggcgca tccacccggc gcgcatcgag gaggtggtcg agaaggtgaa ggccgagctc
     6481 gaggagagcg tgcgccgcga gggcgaggcg gccgcgttcg agatgggcat ccacgacctc
     6541 caccccgagg tgcaccgcct gatggggcgc ctcaagttcc gcaccagcta cgggcagaac
     6601 gtcctcaccc actccaagga ggttgcgtac ctcgcgggca tgatggcgcg cgagctcgac
     6661 ctcgactggc acacgaccgt gcgggccgcc ttcctccacg acatcggcaa ggcgatcgac
     6721 cgggagatgc agggcaccca cctcgagatc ggcgtcgact tccttcgccg ccacggcgag
     6781 accccggcca tcaccgcgat cgtcgcgtcg caccacatgg acaccgactg gccgtgcgtc
     6841 gaggccatgc tggtgcaggc ggccgacgcg atctccgcgg cgcggcccgg cgcccgccgc
     6901 gacatcctcg agtcgtacgt caagcggctc gagaagctcg agagcatcgc cgactccttc
     6961 aagggcgtcg cgaagtcgtt cgcgctgcag gccggccgcg agatccggat catggtcgag
     7021 agcgagcgga tcaccgacga ggaagccgtc tggctctcga aggacatcgc gcggcgcatc
     7081 gagagcgagc tcgaataccc cggccagatc aaggtcaccg tcatccgcga gacgcgggcc
     7141 gtggagtacg cgaaatagcg tgccagcgcc caaggtcggc gacatcggcg agcgcgaggt
     7201 gatccgccgg attcgccggc ggctgcccgc cccaccagac tggctgcgca cccccatcgg
     7261 cgacgacgcg gccgtcgtgc gcccgccccg gaaccgcgac gaggtggtca ccaccgacgc
     7321 gatggtcgag gggattcact tcgaccggtc gtacgtcccc cccgccgcga tcggcgccaa
     7381 ggccctcgcc gtcaacctca gcgacctggc cgcgatgggc gccgagcccc gcctggccct
     7441 gttgtcgctg atcctcccgg ccgatacgac cgttagcgac ctcgagtcgc tcgtcgacgg
     7501 catgctggga atggcggcgc gccacggcgt cacgctggcc ggcggcaacg tcgcgcgctc
     7561 ccccggcccc ctggtcgtcg acctcaccgc gatcgggtgc gtcaagcccc gcgacgtgct
     7621 gcttcgccgc ggcgccaggc ctggagacgg gatctacgtc agcggcgcgg tcggcgcggc
     7681 gcgcgccggc ctgctgtcgc tgcacggtgc gcataacgtc gcggggcccg cggaaggtga
     7741 cggcctggac gaggtgcggg cgcggttcct cgtgcccgag ccccgcgtcc gccttggcct
     7801 gctgctggcg aggaaccgcg ttgcctcggc ctgcatggac ctgagcgacg ggctggccga
     7861 cgccgtccgc ggcgtggcgg ccgcgggccg ggtgggggcg atcgtcgagg ccgaggcgct
     7921 gcccatcccc ggcgcggccc ggcggatctt cgaggttgcg gggctcgatt cgatcgaggc
     7981 ggcgctggag ggtggcgagg actacgagct gctcttcacg gtaccgcgcc gccgccgccg
     8041 ggccctgacc gcggtgctgg cgctcgtccc ggacgtcgcc tgcacgcgca tcggccacgt
     8101 gacggcggag cggcggatcg tgctccggcg ggagggcgtg gaaagggcgc tccccgccgg
     8161 ctactcgcac ttcacgggat gaagggcata cgccattcgc tctggcgcaa gctggtggtc
     8221 acgctgatcg ccggggcggg gttcgtcctc ctctaccagg ccacgatctt cgactcgcgc
     8281 tacgccgcgc gccaggctct gctgcgcgaa gacgtggccg accccggacc cggcacccgc
     8341 cttcgtttcc tcgcgaccgc gtactgcaag ggcgacatca ccgcctcggg cctcccgccg
     8401 cgccacggca tcgccgccgc cgatcccgac ctgctgccgg tcggatcggt agtgcagatc
     8461 gtgcgcggcg ccaagggcta cgagggggtc tacaccatca tggacaccgg cccggtagtg
     8521 cgaggccggc gcctcgacct ctacatctgg agctgcaacg aggcactcca gttcggccgc
     8581 cgctcggtgc aggtggtcgt cctgcgcctc ggctggaacc ccgccgcctc gggcccctcc
     8641 ctgctcgaca agcttctccc ctggcggact cgccagtagc acaggcgtcc cgcctgtgcc
     8701 agcctcacag gcgtcccgcc tgtgcccagc ctcacaggcg tcgcgcctgt gcccagcctc
     8761 acaggcgaga cggctgtgcc cagcctcaca ggcgagacgg ctgtgcccag cctcacaggc
     8821 gagacgcctg tgcccagcct cacaggcgag acgcctgtgc tactggctgc gggtggcctt
     8881 cgggttgcag ctttacggcc gggcgtccga atggtcgggg ccccatcaga aaaggaagta
     8941 tgagaatcgc gcaagttgcc ccgctcttcg agagcgtgcc gccaaagctc tacggcggca
     9001 ccgaacgtgt cgtgtcgtac ctgaccgagg aactcgtccg cctcggccac caggtcacgc
     9061 tcttcgccac cggtgactca cgcacctcgg ccaggctcgt gccggtgtgg ccgcaggcgc
     9121 tgcggctgga cgagacgtgc gccgaccagc tggcgccgca cgtgctgatg atcgagcagg
     9181 tggcacgcat ggcggatcag ttcgacgtca tccattttca tgtggcccag ttgcacctgc
     9241 ccctcgcgcg gcgcctgcac ggcgtggccc acctgaccac gcttcacggc cggctcgacc
     9301 tcccggagct cgtcccgctg ttcgacgagt tttccgagat cccggtcgtc tcgatctccg
     9361 acgcgcagcg ccagcccctg ccgcgcgcgg cctgggttgg caccgtgcat cacggcctgc
     9421 cggccggcct gctgccgttc agccccggcc ccggcgaata cctcgccttc ctcggccggg
     9481 tgtcgcccga gaagggagtg ggccgcgcca tcgcgatcgc cagggcgtgc ggcatcccac
     9541 tccgcatcgc ggcgaaggtg gatccggccg accgcgtgta cttcgagacc gagattcgcc
     9601 cgctgctcga cgacccgctc gtcgagttca tcggcgagat cggcgagaac gaaaaggggc
     9661 cgttccttgg cggcgcgcgg gcactgctct tcccgatcga ctggcccgag ccgttcggcg
     9721 tcgtcatgat cgaagcgctg gcgtgcgggc tcccggtggt ggccttcgag ggcggatcgg
     9781 tgggcgaggt gatctcgaat ggggtgaacg ggttcgtcgt gcgcagcgtg gaggaggcgg
     9841 tgcacgccgt ccaccggatc ggctcgatcg accggcgggc gtgccgcgcc gatttcgaac
     9901 ggcgcttcac cgcctcgcgg atggcggcgg attacctggc gctgtaccgc gcggtccgcc
     9961 gtgcggccgc cgcgttccgc ctgccccgcc ccgccctgtc gcccgccgtc gaggcgcccg
    10021 tggccgccgg cgaggcgccc gtgccggccg gcgagtacgg cggccccgcg aacgggcgca
    10081 gcggcttcac cggtggggca cggcgggcga acgatctcgg cgagagcgga cacctatgac
    10141 gggcccggcg tccaagcccg gaggtgtgcg cctcgacgag ttcgcgatca tcagcggcgg
    10201 cgaccggggg ggcgcgccct cgcgcgtcct gaagcagggc gacaccttcg gcgtcttcga
    10261 tctccacggc gacgcggtcc ccgagggggc gcgcgagcac ggcctctatc acgaggggac
    10321 ccgcttcctg tcccgtctcg agctgcgctt cgggaagagc cggcccctgc tgctcgggtc
    10381 gcggatcagc gacgacaaca ccgtgctcgc ggtcgacctg acgaacccgg acgtggtgaa
    10441 ggacggccgc cccctggtgc ggcgcggcat actgcacgtg ttccgcgcgc gccttctctg
    10501 ggacggccac tgcctcgagc ggattcggct gtcgaaccac gcgctgcacc ccatcgagct
    10561 gccgctgacc ctcgtcttcg acgccgacta cgccgatctc ttcgaggttc gcggcacgca
    10621 ccgcgccagc cggggccggc tcttcgcccc ctcgatcgcc cccgactccg cgctgctccg
    10681 gtacgagggg ctggacggcc tcgagcggcg gacgcgcctc gagtggagcg cggcggccga
    10741 tcgggtcgag gatcacgggg ccaccttctc ggtctccctc gacccgcacg aggtgaggga
    10801 gatcgaactg tcgatcgcct tcgagctggg ggccgtcacg cgcccggccc gaagctacga
    10861 cgcggcgctg tcgtcgctca agggggagat ggcggccctc caggcccgcg agtgcggcgt
    10921 cctcacctcg aacgagagct tcaatcgctg gctccgccgc tcctcggccg acctgcgcat
    10981 gatgctcacc gagaccccgt cgggacccta tccctacgcc ggcatcccct ggtacagcac
    11041 cccgttcggc cgcgacggcc tgctgaccgc gtacgaaatg ctgtgggcgg cgccggacgt
    11101 ggcccggggc gtgctcgcgt ttttggccga gacgcaggcg aaggttgtct ccgacgacca
    11161 ggacgcgcaa cccggcaaga tcatccacga gatgcgggcc ggcgagatgg cggcgctcgg
    11221 ggagatcccg ttcgggcgct actacggcag cgtggatgcc acgcccctct tcgtgatgct
    11281 ggcccacgcc tacttcgggc ggacggccga ccgggcgttc ctcgaccgcc tgtggccgca
    11341 cctgctcgcg gccctggaat ggatggagga atacggcgac ctggacggcg atctcttcat
    11401 cgagtacgcc cgccgcagcc cgtccggcct gctgcaccag gggtggaaag actcgaacga
    11461 ctcggtcttc cacgaggacg gcgagctggc ggaagggccg atcgccctct gcgaggtgca
    11521 ggcgtacgcg tacggcgcct ggaccggcgc cgccgccctt gccgaggtgc ggggcgacct
    11581 ggcccgcgcc cgcgggtggc gcgacagggc cgccgccctt cgcgagcggt tcgatcacgc
    11641 cttctggtgc gagccgctcg ggacctacgc cctcgcgctc gacgggcaga agcgcccctg
    11701 ccgggtgcgg acctcgaacg ccgggcattg cctgttcagc ggcctcgcga cgcggccccg
    11761 cgcccggagc gtg
//
LOCUS       DAOPQX010000080        11761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6299_length_11761_cov_4.982658, whole genome shotgun sequence.
ACCESSION   DAOPQX010000080 DAOPQX010000000
VERSION     DAOPQX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11761
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6299_length_11761_cov_4.982658"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKK95_06290"
     CDS             <1..299
                     /locus_tag="PKK95_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17854.1"
                     /translation="GLGISVDGDRIPIRPETRAICDALGVDPLRLIASGALLAATRAS
                     DAGPVLDVLQDLSIPAAVVAELRAAPEGRVISLAGRTQPLELPARDEIARLFEQ"
     gene            546..956
                     /locus_tag="PKK95_06295"
     CDS             546..956
                     /locus_tag="PKK95_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC17855.1"
                     /translation="MADQSSRKLFVNLPVRDLKRAMEFFGALDFRFNPKFTDENAACM
                     ILSDEAYVMLLAEPFFRTFTKREPADTSRYTEALLALSCGSRAEVDELVGKAVAAGGS
                     HAMDPTDHGFMYGWSFYDPDGHHWEVFWMDPAAR"
     gene            953..2701
                     /locus_tag="PKK95_06300"
     CDS             953..2701
                     /locus_tag="PKK95_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOC17856.1"
                     /translation="MTRRPKPSGATGLVREDDGVSARDVLEAAPDALVVADEAGTIVR
                     VNLAAEQLFGYAPGELTGKPVEVLVPEVYRDAHVGHRLGYNRRMRRRPMGSGPELYGL
                     RRDGSEFPVEISLSPLMTARGTVVIAAVRDISARKQAEAERSHLIRERALYAEISRLA
                     RRDALTGLPNRTLLVDRVGAAIASAQRHQHKLAVIFLDLDRFKHVNDSLGHAVGDRLL
                     RSVASRLTGAVRASDTVSRQGGDEFVILLSEVRHREDVVVAAGKIVAALAQPHQVDGQ
                     DLYVTASLGIAVYDEDGADVHTLIKNADVAMYYAKDHGRDNYQFFTPEMNARIVERQA
                     LEGNLRRAIERREFLLYYQPKVDLETGRMIGAEALIRWQHPVRGLLPPQRFVPVAEDS
                     GLIVPIGQWVLHEACRQAREWQAAGLHPVPIAVNISALEFRSKNFLERVRRTLDETGL
                     EPRFLELELTESVLMESVDETAEVLRELKAMGLRLAVDDFGTGYSSLSYLTQFPIDAL
                     KVDQSFVRDIAPRDGSPIITAVISMGKSLNQRVIAEGVETWVQVEFLRAQQCEEAQGF
                     HFGRPLAADQFAALLG"
     gene            complement(2749..3333)
                     /locus_tag="PKK95_06305"
     CDS             complement(2749..3333)
                     /locus_tag="PKK95_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOC17857.1"
                     /translation="MNGNLNGKRIAILVEQGFEQVELVEPRKALEQAGARVEIVSPRQ
                     GKVRGWNHTEWGDEVGVDKPLDSAREADYDGLMLPGGVMNPDKLRMNEKAVQLVRAFF
                     DAGKPIAAICHAPWLLAEAGVVGGVRLTSYPSLKTDLRNAGAEWADEECIVDRGLVTS
                     RKPDDIPAFNRKMIEEFAEGRHSGRRTMTAGGTR"
     gene            3478..4725
                     /locus_tag="PKK95_06310"
     CDS             3478..4725
                     /locus_tag="PKK95_06310"
                     /inference="COORDINATES: protein motif:HMM:NF021534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HOC17858.1"
                     /translation="MQELTPRRTFLKLAAASGTVAMGLRQPGTLAQEGGAVAGGAAER
                     AWWITVLRRLADPVLENLAAGTLRSRMPVEGPANRRAVTHLEAVGRLLAGIAPWLELG
                     GDTTAEGAARARYANLARRALGVGLDRSSPDALNFTEGGQPLVDAAFLAQAILRAPRA
                     LWQALEPSTQASLVAALESTRAIQPGFNNWLLFSATVEAALARMGARWDRVRVDYALR
                     QHEQWYKGDGIYGDGPELHWDYYNSFVIQPMLLDVLDACGGTSPAWQALAGPVARRAQ
                     RYAAIQERLIAPDGSWPLVGRSLAYRTGAFQLLAQVALRHALPAGLTPCSVRSALTAA
                     MRRSLEAPGTFDTDGWLHIGVCGHQPVIGERYISTGSLYLCAAVLLPLGLPEADEFWS
                     GPPAPWTSVLAWSGKEFPIDHAG"
     gene            4841..6046
                     /locus_tag="PKK95_06315"
     CDS             4841..6046
                     /locus_tag="PKK95_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC17859.1"
                     /translation="MTSRERMRVAMRLGQPDRVPVMCQLAIGHYFLHAGADPLETWFS
                     SEGFGEALIALQRRYRFDGILVNLPGRPPGWRRHARSIERDEGKSVIRWKNGWSTVFP
                     HDDLPWVLRQDGSRFRPPFAEIDPEALFYVEPHGPLGVRYPSVPGFEEGVAGAEARSV
                     QAFPSYQYDTIAYVRRHAGEVSVHGEVFSPFTQLFELADHASVLMALMTDPGKVRACL
                     QRLTAAAIALAIGQAAAGADAILISSAFAGAGFISPAHYREFVLPYEKQVIDAVRRYH
                     DVPIYTHTCGAIGDRLELMMESGTNGIDTLDPPPLGTVHLADARRRTLGRVFLKGNID
                     PVNTMLLGTPDGVRAAARECLSVAGRSGGYILSTACAVPPATSPANILALADAVTAGV
                     RDGPLKGGS"
     gene            6141..6926
                     /locus_tag="PKK95_06320"
     CDS             6141..6926
                     /locus_tag="PKK95_06320"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC17860.1"
                     /translation="MAIETGLAGKVVIVTGAAAGIGRATAACFAREGARVAAWDVVGA
                     QAALLVDELKAAGGHAMFTAVDVTDAAAVNRAVEETVTAWGRVDVLVNNAGILRDGLL
                     VKWKDGALTGQLGDEQFEAVIDVNLKGPFLCTRAVVPHMIRGGGGVILNASSVVGLYG
                     NFGQTSYVASKAGVIGMTRVWARELGRHGIRVNAVAPGFIGTEMVRQMPGKVLDTMVS
                     KTPLGRLGRPEEIGNAYVWLASEAASYVTGAVISVDGGVVTGT"
     gene            complement(6927..>10082)
                     /locus_tag="PKK95_06325"
     CDS             complement(6927..>10082)
                     /locus_tag="PKK95_06325"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOC17861.1"
                     /translation="TETRTNVSYDTVTNATGNYTFPNLPEGVYNVKAALEGFKTTVRE
                     GVRVAPNTSVRIDLALEVGGLEETVTVTGETPLLQTDRTDIGRTIESIQVSAMPLSFN
                     RNFQGMMATVPGSTRPYRPHSEFFNSQDSLSSEVNGQSRLANNVQIEGLDNNQRTGLL
                     TVLIPPAEAIETVSVTTSNYDAEFGRAAGAVTSVTLKSGTNTLKGSAFWFGSNEKFNS
                     LPASTHFSPAASRSKPPTRYNQFGFTLGGPVRKNRLFFFGDYQRTQDRLGSAYRFVVP
                     TAAFRNGDFSAAPTKVYDPATGDAAGNGRTEFPGNQIPADRISPIAIAILKNIPMPNL
                     ADAGLGQANYQVNSYRDKNTDSFDTKLNYALNDSNQFSWRFSYQRPEITQLPAEGYGN
                     WGGPLGGGFMATGTNMTYSTAANWTHTFSNTFLMEARGGTSYYHNEALTTAHGEKLAE
                     QLGIPGVNLDDWTSGPPAINIQNGFSNPMMGYVNSLPWDRWERTWEFATTLTKIRGNH
                     TVKFGATWRHNKDKLLQTQDNQGPRGGFTFGGAQTGSPADTAADSGIANAFASFLLDR
                     PSGMARDLKVLDDVGSQHWAFYTFVHDKWQATSKLTVDLGLRWEYYDPIVGLAGKGSL
                     SNYDPATNSLLVSGYGDVADDFGIKRDYNNFNPRLGLSYRVNEKTVVRGGFGASTTPF
                     PDNRYAFNYPVKQNNSYQPPNPYSVAGSMAAGFPAPTYLTIPDNGIVDANLVKASSLF
                     HVPNDLQQGSLYSWNVAFQRELIWGLTGEVAYVGNRSNDVLNRFPMNAGLQLGAGVDG
                     QPLYVKYGKTAGVENLAWKGKTRYNGLQLKLDRRFRGGWLVTNSYTYARSRDYANENG
                     GVSTPADIERSWGYSNFDRRHVYVSTFVWSLPWFAEREQGFLHYLLGNWQLSGIFTYQ
                     SGQVLDVTAASSASLNTPGNTQRPNSSGAPAIIGDFGPGKYYFDTSVFSAPAANTWGN
                     RTRNMGDLRGPRFTNLDVSVAKQFGLGGSRIAQIRLDVWNLPNTAHMNNPNTSFGSST
                     FGQVTSAYNERQMRLSARFLF"
     assembly_gap    10083..10182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10309..11151)
                     /locus_tag="PKK95_06330"
     CDS             complement(10309..11151)
                     /locus_tag="PKK95_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17862.1"
                     /translation="MHALTPLLLVLALAAVPAQPSRPPAPVGAKADYHVHVKGGLTLE
                     GALQRSQETGITYGIVVNGGVGFPIQDDTGLEAFLRETRGKAAYVAFQAEGREWAGLF
                     GRATLEKFDYIFTDAMTWTDDKGRRMRLWIPGDVGRIDDPQAFMDMLVDRASGIFAGE
                     PIDLYVNPTYLPDQLAHDYDRLWTPARMKRIVDGLAANGIGMEINNRYRIPSLAFVRL
                     AKQAGIKFACGTNNAGADDLGRNEYCAQIIEEAGLGPADFWTPPAEGQKAIQRRPRTG
                     GARH"
     gene            complement(11183..>11761)
                     /locus_tag="PKK95_06335"
     CDS             complement(11183..>11761)
                     /locus_tag="PKK95_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC17863.1"
                     /translation="RMGVANIINQERDLEVVAEAGTGTEAVAAYERHRPDVVLLDLRM
                     PVMEGVEVVRRIREIDAQARVVVLTTYDTDDEIARALKAGAKAYVLKDISADDLVSCI
                     RTVLAGKTYLAPAAAAKLAEGVTRVQLTPREMTTLRLLADGKANKEIASELRISERTV
                     KTHLGHLFEKLGVTSRTEALKVAGRRGLVRFE"
BASE COUNT         1679 a         3826 c         4209 g         1947 t
ORIGIN      
        1 ccgggctggg catctcggtg gacggcgatc gcatcccgat ccgccccgag acgcgggcga
       61 tctgcgacgc tctcggcgtc gatccgcttc ggctcatcgc ctcgggcgcg ctcctggcag
      121 ccacgcgcgc ctccgacgcc ggccccgttc tcgatgttct tcaggacctg tcgatccccg
      181 ccgccgtggt cgccgaactg cgcgccgcgc ccgaaggccg ggtcatctcg cttgccggcc
      241 ggacgcagcc gctcgagctg ccggcacggg acgagatcgc acgcctcttc gagcagtgac
      301 ccagtagcac aggcgtcctg cctgtgagac gcagtgacaa ctcagtggca caggcgtccc
      361 gcctgtgggg cctagtagca caggcgtctc gcctgtggag cccagtggca caggcgtccc
      421 gcctgtggga cccagtagca caggcgtccc gcctgtgcgc gatcggcacg aatcgtgaaa
      481 gacggcccgt ctgctccgcg ctgcaatgtt gccgcgggcc ttgaacgacc tggagcacgc
      541 agacgatggc cgaccagtcc tcgcgcaagc tgtttgtcaa cctgccggtg cgcgacttga
      601 agagggcgat ggaattcttc ggcgccctcg attttcgctt caatccgaag ttcaccgacg
      661 agaacgcggc ctgcatgatc ctcagcgacg aggcgtacgt catgctgctc gccgagcctt
      721 tctttcggac cttcacgaag agagagccgg ctgacacgtc ccgctacacg gaagcacttc
      781 ttgcgctctc ctgcgggagc cgcgccgagg tggacgagct ggtggggaag gccgtcgccg
      841 cggggggctc gcacgccatg gacccgacgg accacggctt catgtatggc tggagcttct
      901 acgatccgga cggccaccac tgggaggtct tctggatgga cccggcggcg cgatgacgag
      961 gcggccaaag cccagcggag cgacgggtct cgtgcgcgag gatgacggtg tttcggcgag
     1021 ggacgtgctc gaggcggctc ccgacgcgct ggtcgtcgcc gacgaggccg gcacgatcgt
     1081 cagggtcaac ctcgcggccg agcagctgtt cggctacgcg ccgggggagc tgacgggcaa
     1141 gccagtggag gtgctcgtgc ccgaggtgta ccgggacgcg cacgtcggcc accggctggg
     1201 ctacaaccgc aggatgcggc gcaggccgat gggcagtggg cccgagctgt acgggctgcg
     1261 aagggatggc agcgagttcc cggtcgagat cagtctgtcg ccgctgatga ccgcgcgggg
     1321 caccgtcgtc atcgcggccg tccgggacat ctcggcgagg aagcaagcgg aggccgagcg
     1381 cagtcacctg atccgggagc gggcgctcta cgcggagatc tcgcggctgg ccaggcggga
     1441 cgccctcacc ggcctgccca accgcacgct gctggtggac cgggtggggg cggccatcgc
     1501 ctcggcccag aggcaccagc acaagctggc ggtgatcttc ctcgacctcg accgcttcaa
     1561 gcacgtcaac gattcgctcg gccacgcggt gggtgaccgg ctgctgcggt cggtggcgtc
     1621 gcggctcacg ggcgccgtgc gggccagcga cacggtgagc cggcaggggg gcgacgagtt
     1681 cgtgatcctc ctctccgagg tccggcaccg ggaagacgtc gtcgtagcgg ccgggaagat
     1741 cgtggcggcg cttgcgcagc cgcaccaggt ggacgggcag gacctgtacg tcacggccag
     1801 tctcgggatt gccgtgtacg acgaggacgg cgccgacgtt cacacgctga tcaagaacgc
     1861 cgacgtcgcg atgtactacg cgaaggacca cgggcgcgac aactaccagt tcttcacgcc
     1921 cgagatgaac gcccggatcg tcgagcggca ggcgctcgag gggaaccttc gccgggcgat
     1981 cgagcggcgc gagttcctcc tctactacca gccgaaggtt gacctcgaga cgggccggat
     2041 gatcggcgcc gaggcactga tccggtggca gcacccggtg cgggggctgc tgccgccgca
     2101 gcggttcgtg ccggtggccg aggacagcgg cctgatcgtg ccgatcgggc agtgggtgct
     2161 ccacgaggcg tgccgccagg cgcgcgagtg gcaggcggca ggcctgcacc ccgtgccgat
     2221 cgcggtgaac atctcggcgc tcgagttccg gagcaagaac ttcctcgagc gcgtgaggcg
     2281 cacgctggac gaaacgggac tcgagccgcg gttcctcgag ctggagctga ccgagagcgt
     2341 gctgatggag agcgtggacg agaccgccga ggtgctgcgg gagctgaagg cgatggggct
     2401 gcggctcgcg gtcgacgact tcgggaccgg ctactcgagc ctgagctatt tgacgcagtt
     2461 cccgatcgac gccctgaagg tggaccagtc gttcgtgcgc gacatcgcgc cgcgggacgg
     2521 gtcgccgatc atcacggcgg tgatcagcat gggcaagagc ctcaaccagc gggtgattgc
     2581 cgagggcgtc gagacctggg tgcaggtgga gttcctgcgg gcgcagcagt gcgaggaggc
     2641 gcagggcttc cacttcggcc gcccgctcgc ggccgaccag ttcgcggcgc tgcttgggta
     2701 ggcagccaac gagtgagaaa tggggccagg ccccatttct caggcctctt accgcgtgcc
     2761 tcccgctgtc atggtgcggc ggccggagtg cctcccttcg gcgaactcct cgatcatctt
     2821 ccggttgaag gccgggatgt cgtcgggctt cctcgacgtc accagccccc ggtccacgat
     2881 gcactcctcg tccgcccact cggcgccggc gttccgaagg tcggtcttga gggagggata
     2941 cgacgtcagg cggacgccgc cgacgacgcc ggcctcggcc agcagccagg gggcgtggca
     3001 gatggccgcg attggcttgc ccgcgtcgaa gaaggcgcgc accagctgca cggccttctc
     3061 gttcattcgc agcttgtcgg ggttcatcac gccgcccggc agcatcaggc cgtcgtagtc
     3121 ggcctcgcgc gccgaatcga ggggcttgtc cacccctacc tcgtcacccc actcggtgtg
     3181 gttccagccg cggaccttgc cctgccgggg tgagacgatc tcgactcttg cgccggcctg
     3241 ctcgagggcc ttgcgcggct cgacgagctc gacctgctcg aatccctgct cgaccaggat
     3301 cgcgatgcgc ttgccattca ggttgccgtt cacgaggcct acctcctctg atgtgctcaa
     3361 tccaaaggga aatggaggac gcgtccgcac gacgcgcaac caggaaggtg tatcaagatg
     3421 cgggccaggg cgagagatcc tcgtgaatat ccgggccggg acgtgcacaa tgcgggcatg
     3481 caggagctga ctcctcggag aaccttcctg aagctggcag cggcgagcgg gaccgtcgcg
     3541 atggggctgc ggcagccggg aacgttggcg caggaaggcg gcgccgttgc cggcggtgcc
     3601 gcggaacgcg cgtggtggat cacggtgctt cgccgtcttg ccgacccggt gctcgagaac
     3661 ctcgccgcgg gaacgctcag gtcgcggatg ccggtcgagg ggccggccaa ccggcgcgcg
     3721 gtgacccacc tcgaggcggt tgggcggctc ctggccggga ttgcgccgtg gctcgagctg
     3781 ggtggcgaca ccacggccga gggggctgcg cgtgcgcggt acgccaacct tgcccgccgc
     3841 gccctcggcg tcggcctcga ccggtcttca cccgacgcgc tgaacttcac cgaggggggc
     3901 caaccactcg tcgacgccgc gttcctcgcg caggcaatcc ttcgcgcccc gcgggcgctg
     3961 tggcaggcgc tcgagccgtc cacgcaggcc tctctcgtcg ccgccctgga atcgacgcgc
     4021 gcgatccagc cggggttcaa caactggctc ctcttctcgg ccacggtcga ggcggcgctt
     4081 gcacgcatgg gcgcgcgatg ggaccgggtg cgcgtggact acgcgctgcg gcagcacgag
     4141 cagtggtaca agggcgacgg catctacggc gacggcccgg agctccactg ggactactac
     4201 aacagcttcg tgatccagcc gatgctgctc gacgtgctcg atgcgtgcgg cggcacctcc
     4261 cccgcgtggc aggcgctggc cgggccggtc gccaggcgcg cgcagcgcta cgcggccatc
     4321 caggaacggc tcatcgcacc cgacggcagt tggccgctcg tggggaggtc gctcgcctac
     4381 cgcacgggag cgttccagct gctggcgcag gtggcgctgc ggcacgcgct gccggcgggg
     4441 ttgacgcctt gctcggtccg ctcggccctg acggcggcga tgcggcgatc gctcgaggca
     4501 ccgggcacgt tcgacactga cgggtggctg cacatcggcg tctgcgggca ccagccggtc
     4561 atcggcgagc ggtatatctc gaccggcagc ctgtacttgt gcgcggcggt gctgctgccg
     4621 ctgggccttc cggaagccga cgaattctgg agcgggccgc cggcgccgtg gacgtcggtg
     4681 ctcgcctggt cggggaagga gttcccgatc gaccacgcgg gctgagaaga cggctcacgc
     4741 ctcacggctc aaggctcagg gcttgcccgc cgtagccgcg agagtagcga gcgtttggcg
     4801 aaggcgggct cacgggcttg gggcgggcgg aaggcaggag atgaccagtc gcgagcgaat
     4861 gcgggtggcg atgcggctcg gccagccgga tcgggtcccg gtgatgtgcc agctggccat
     4921 cggccactac ttcctccacg ccggggccga tccgctcgaa acctggttct cgagcgaggg
     4981 attcggggag gcgctgatcg cgctccagcg gcgctaccgc ttcgacggca tcctggtgaa
     5041 cctgccgggg cgcccgccag gctggcgccg gcacgcacgc tccatcgagc gcgacgaggg
     5101 caagagcgtc atccgctgga agaacggctg gtccacggtc tttccgcacg acgatcttcc
     5161 gtgggttctt cgccaggatg gctcgcgctt ccgcccgccg ttcgcggaga tcgatcctga
     5221 ggcgctcttc tacgtggagc cgcacgggcc gctcggggtg cgctacccgt ccgtgccggg
     5281 gttcgaggag ggggtggcgg gcgctgaggc gcggtccgtc caggcattcc cttcctacca
     5341 gtacgacacc atcgcctacg tgcgacggca cgccggcgag gtgtcggtgc acggcgaggt
     5401 cttctcgccc ttcacgcagc tgttcgagct ggccgaccac gcgtcggtgc tgatggcctt
     5461 gatgaccgac ccgggtaagg ttcgcgcgtg cctccagcgg ctgacggcgg cagccattgc
     5521 ccttgcaatc gggcaggctg ccgccggggc ggatgccatc ctgatctcgt ccgccttcgc
     5581 cggcgccggc ttcatctcgc ccgcccacta ccgggagttc gtcctgccgt acgagaagca
     5641 ggtgatcgac gccgtgcgga ggtaccacga cgttccgatc tacacgcaca cctgcggcgc
     5701 catcggcgac cggctcgagt tgatgatgga gagcggcacg aacggcatcg acacgctcga
     5761 tcccccgccg ctcggcaccg tgcatctcgc cgacgcgcgc cgccggacgc tggggcgcgt
     5821 gttcctcaag ggcaacatcg acccggtgaa cacaatgctt ctcgggacgc ccgatggcgt
     5881 ccgggccgcg gcacgggagt gcttgtcggt ggcgggacgg agcggcggct acattctgag
     5941 caccgcgtgc gcggttccgc ccgccacctc gcccgcgaac atcctggcgc tggctgacgc
     6001 cgtgacagct ggcgttcgtg atggcccatt gaagggcggg tcgtgacgag cctccgtgtc
     6061 tctgtgcctc tgtggttgac gtggacaagt agtgcagtgt gggatgggaa tcgaaattgg
     6121 aatcggcagg cggagggagt atggcgatcg agacggggct ggcggggaag gtggtgatcg
     6181 tgaccggcgc ggcagccggg atcggccggg caaccgcggc gtgcttcgcc agggaagggg
     6241 cgcgcgtggc ggcctgggac gtcgtgggcg cgcaggccgc ccttctggtg gacgagctga
     6301 aggcggccgg gggccacgcg atgttcaccg ccgtggacgt caccgacgcg gccgccgtca
     6361 accgcgccgt cgaggagacg gtgacggcgt ggggccgggt ggacgtgctc gtgaacaacg
     6421 ccggcatcct gcgcgacggg ctgctcgtga agtggaagga cggggcattg accggccagc
     6481 ttggcgacga gcagttcgag gcggtgatcg acgtcaacct gaaggggccg ttcctctgca
     6541 cccgcgcggt cgtcccgcac atgatccggg gcggcggggg cgtgatcctc aacgcctcgt
     6601 cggtggtcgg cctctacggc aacttcggcc agaccagcta cgtggccagc aaggcgggcg
     6661 tcatcggcat gacgagggtg tgggcgcgcg agctggggcg ccacggcatt cgcgtgaacg
     6721 ccgtggcgcc cgggttcatc ggcacggaga tggtccgtca gatgcccggg aaggtgctcg
     6781 acacgatggt gtcgaagacc cccctggggc ggctcggccg ccccgaggag atcggcaacg
     6841 cctacgtgtg gctcgcctcc gaggccgctt cctacgtcac cggcgccgtc atctcggtgg
     6901 atggcggcgt ggtgacggga acgtaactag aacaggaacc tcgcgctcag gcgcatctgt
     6961 cgctcgttgt acgccgaggt cacctggccg aacgtcgagc tgccgaacga ggtgttcggg
     7021 ttgttcatgt gcgccgtgtt cggcaggttc cagacgtcga ggcggatctg cgcgatccgg
     7081 ctcccgccca gcccgaactg cttggccacg ctcacgtcca ggttggtgaa gcgcggcccg
     7141 cgcaggtcgc ccatgttgcg ggtgcggttg ccccacgtgt tggcggcagg cgccgagaac
     7201 accgacgtgt cgaagtagta cttgcccggg ccgaagtcgc cgatgatggc cggcgcgccc
     7261 gagctgttgg gccgctgcgt gttgccgggg gtgttcagcg aggccgagct ggccgccgtc
     7321 acgtcgagca cctggcccga ctggtaggtg aagatccccg acagctgcca gttgcccagc
     7381 aggtagtgca ggaagccctg ctcccgctcg gcgaaccagg gcagcgacca gacgaaggtc
     7441 gagacgtaga cgtggcggcg gtcgaagttc gagtagcccc agctgcgctc gatgtcggcc
     7501 ggcgtgctca cgcctccgtt ctcgttggcg tagtcgcgcg acctggcgta ggtgtacgag
     7561 ttggtgacga gccacccgcc ccggaatcgc cggtccagct tgagctgcag cccgttgtac
     7621 cgcgtcttac ctttccacgc gaggttctcg acgcccgcgg tcttgccgta cttcacgtag
     7681 agcggctggc cgtcgacgcc cgcgccgagc tgcaggcccg cgttcatcgg gaagcgattg
     7741 agcacgtcgt tgctgcggtt gccgacgtag gccacctcgc cggtcaggcc ccagatcagc
     7801 tcgcgctgga acgccacgtt ccacgagtac agcgacccct gctgcaggtc gttcggcacg
     7861 tggaacagcg aggaggcctt caccaggttg gcgtccacga tcccgttgtc gggaatcgtc
     7921 aggtaggtcg gggcggggaa gcccgcggcc atcgatcccg ccaccgagta cgggttgggc
     7981 ggctggtagc tgttgttctg cttgaccggg tagttgaacg cgtaccggtt gtcggggaac
     8041 ggcgtggtgc tcgcgccgaa gccgccgcgc accacggtct tctcgttcac ccggtacgag
     8101 aggccgaggc gcgggttgaa gttgttgtag tcgcgcttga tgccgaagtc gtcggcgacg
     8161 tcgccgtagc ccgagacgag cagcgagttg gtggccgggt cgtagttcga cagcgagccc
     8221 ttgccggcga ggccgacgat cgggtcgtag tactcccacc tcagcccgag gtccacggtg
     8281 agcttgctgg tcgcctgcca cttgtcgtga acgaaggtgt agaacgccca gtgctgcgag
     8341 cccacgtcgt cgagcacttt caggtcgcgc gccatgccgc tcggccggtc gagcaggaac
     8401 gaggcgaagg cgttcgcgat gccgctgtcc gcggccgtgt cggccggcga cccggtctgc
     8461 gccccgccga aggtgaagcc gccgcggggc ccctggttgt cctgcgtctg cagcagcttg
     8521 tccttgttgt ggcgccaggt cgcaccgaac ttgacggtgt ggttcccccg gatcttcgtc
     8581 agcgtggtgg cgaactccca ggtgcgctcc cagcggtccc acggcaggct gttgacgtag
     8641 cccatcatcg ggttcgagaa cccgttctgg atgttgatgg ccggcggccc gctcgtccag
     8701 tcgtccaggt tgacgcccgg gatcccgagc tgctcggcca gcttctcgcc gtgcgcggtg
     8761 gtcagcgcct cgttgtggta gtagctggtg ccgccgcgcg cctccatcag gaaggtgttc
     8821 gagaaggtgt gcgtccagtt ggcggccgtg ctgtaggtca tgttggtgcc ggtcgccatg
     8881 aacccgccgc cgagaggccc gccccagttc ccgtatccct ccgcggggag ctgggtgatc
     8941 tcgggacgct ggtagctgaa gcgccacgag aactggttcg agtcgttcag cgcgtaattc
     9001 agcttcgtat cgaagctgtc ggtgttcttg tcgcggtagc tgttcacctg gtagttggcc
     9061 tggccgagcc ccgcgtcggc caggttcggc atcgggatgt tcttcaggat cgcgatcgcg
     9121 atcggactga tgcggtcggc cgggatctgg ttgcccggga actccgtccg gccgttgccg
     9181 gcggcgtcgc cggttgccgg gtcgtagacc ttcgtcggcg cggcgctgaa gtcgccgtta
     9241 cggaacgcgg ccgtcggcac gacgaagcgg tacgccgacc cgaggcgatc ctgcgtccgc
     9301 tggtagtcgc cgaagaagaa gagcctgttc ttgcggacgg gaccgcccag cgtgaagccg
     9361 aactggttgt agcgcgtcgg cggcttcgac cgcgacgccg ccggcgagaa gtgcgtggac
     9421 gccggcagcg agttgaactt ctcgttgctc ccgaaccaga acgcgctgcc cttcagggtg
     9481 ttggtgcccg acttgagcgt caccgacgtc acggcgcccg cggcccggcc gaactcggcg
     9541 tcgtagttgc tcgtcgtcac gctcaccgtc tcgatggctt cggccggggg aatgagcacc
     9601 gtcagcaggc cggtccgctg gttgttgtcc aggccctcga tctgcacgtt gttggcgagc
     9661 cgcgactggc cgttcacctc cgaggagagg ctgtcctggg agttgaagaa ctccgagtgc
     9721 ggccggtacg ggcgggtcga gccggggacc gtcgccatca tgccctggaa gttcctgttg
     9781 aacgacagcg gcatggccga cacctggatg ctctcgatcg tccggccgat gtcggtgcgg
     9841 tcggtctgca gcagcggtgt ctcgccggtg accgtcacgg tttcctcgag acctccgacc
     9901 tcgagcgcga gatcgatgcg gacgctggtg ttcggcgcca cccggacgcc ctcccgcacc
     9961 gtcgtcttga agccctcgag tgccgccttc acgttgtaga cgccctcggg gaggttcggg
    10021 aacgtgtagt tgccggtggc gttggtgacg gtgtcgtagg agacgttggt ccgggtttcc
    10081 gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgggatc cgccaggatg
    10201 aggctgagca ggagagtggt ggagtcgggc taaccgattt gtgccggttg agctaggcgt
    10261 gagcgggtga cgggtgacgc gcgaacgcgc acgttcttca gcggcctgct agtgccgggc
    10321 gccaccggtg cgcggcctgc gctggatggc cttctgcccc tccgccggcg gcgtccagaa
    10381 gtcggcgggg ccgaggccgg cctcctcgat gatctgagcg cagtattcgt tgcggcccag
    10441 gtcgtcggcg ccggcgttgt tggtcccgca cgcgaacttg atcccggcct gcttcgccag
    10501 cctgacgaac gccaggctcg gaatccggta ccggttgttg atctccatcc cgatgccgtt
    10561 ggcggcgagc ccgtcgacga ttcgcttcat ccgggcaggc gtccacagcc ggtcgtagtc
    10621 gtgcgccagc tggtcgggca ggtaggtcgg gttgacgtag agatcgatcg gctcaccggc
    10681 gaaaatcccc gacgcgcgat ccaccagcat gtccatgaac gcctgcgggt cgtcgatccg
    10741 gccgacgtcc ccggggatcc acaaccgcat gcggcggccc ttgtcgtcgg tccaggtcat
    10801 cgcgtcggtg aagatgtagt cgaacttctc gagcgtcgcc cggccgaaga gcccggccca
    10861 ctcgcgcccc tcggcctgga aggcgacgta ggccgccttg ccgcgcgtct cgcgcaggaa
    10921 cgcctcgagc cccgtgtcgt cctgaatcgg gaaccccaca cccccgttga cgacgatgcc
    10981 gtaggtgatg ccggtttcct gcgagcgctg cagcgcgcct tcgagggtca gtcccccctt
    11041 cacgtgaacg tggtagtcgg ccttcgcgcc caccggcgcg ggcggacggg agggctgggc
    11101 gggaacggca gcgagggcca ggacgaggag cagcggggtc agggcgtgca tggcggcatt
    11161 atgcacggcc cggcggatcg cgtcactcga atcggacgag gccgcggcgg ccggcgacct
    11221 tgagcgcctc ggtgcggctc gtcaccccca gcttctcgaa caggtgcccg aggtgcgtct
    11281 tgacggtccg ctcgctgatg cgcagctcgc tcgcgatctc cttgttcgcc tttccatcgg
    11341 ccaggagccg cagcgtcgtc atctcccgcg gggtgagctg gacgcgggtg accccctcgg
    11401 cgagcttggc ggcggcggcc ggggccaggt aggtcttgcc ggccagcacc gtgcgaatgc
    11461 acgagacgag gtcgtctgca gagatgtcct tgaggacgta cgcctttgcg ccggccttga
    11521 gggcgcgcgc gatctcgtcg tcggtgtcgt aggtcgtgag cacgaccacg cgcgcctgcg
    11581 cgtcgatctc gcggatgcgg cggacgacct cgaccccctc catcaccggc atgcggaggt
    11641 cgagcaggac gacgtcgggg cggtggcgct cgtaggccgc gacggcctcg gtaccggtcc
    11701 cggcctcggc aaccacctcc aggtcgcgct cctggttgat gatgttggcc acgcccatcc
    11761 t
//
LOCUS       DAOPQX010000081        11684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6352_length_11684_cov_4.486198, whole genome shotgun sequence.
ACCESSION   DAOPQX010000081 DAOPQX010000000
VERSION     DAOPQX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11684
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6352_length_11684_cov_4.486198"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1400)
                     /locus_tag="PKK95_06340"
     CDS             complement(66..1400)
                     /locus_tag="PKK95_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17864.1"
                     /translation="MSHVSRRTFIKTTGLGLAAAGASSASSYASILGANDRVRVAVVG
                     FSDRARSSLIPAFLQHATAENFELVAVSDLWSLRREQAMAHVEKLCGRRPDVVRNNDE
                     LYSRKDVDAVIIATADFQHALHGVEAVRSGRDAYIEKPLANTMEDARAIRQAVKDTGR
                     VVQIGTQRRSEPVYQRACEFIQSGRFGDIVSVEMTWNVNQPGRWRRPAVVKQLREADT
                     DWKRYLMNRPFEPFDPRKYLEFRLFWPYSSGIPDQWLVHQIDTVHWFTKLPRPRSVVA
                     NGGIYLWKDGRRNWDTMTAVFDYGPLDDASKGFQVVYSSRMTNSAGGTKELYYSNGGT
                     LDLDKNLVTPAGGLGEREAAAMGMPPNLLAGQTLVENATTLETAADTGVDTATLANML
                     DWMRCVRSRKTPNAGIEAGYSHSVALCMTIAAVQTGRRVTFDDAKQEVVTEG"
     gene            1559..2914
                     /locus_tag="PKK95_06345"
     CDS             1559..2914
                     /locus_tag="PKK95_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17865.1"
                     /translation="MSRHDSIGRRQFLEETTLLAAVASGLGAARPAAAQINNSAALPV
                     KPVRVGFVGVGIRGTLLMEAVKGIAGAEIVAAADCYKGHLDRAKELISPSLQTTGDYR
                     QVVTRPDVDAVVIATPDHWHLQMTREALAAGKHVYIEKPMTHSWEEGEAFIEAAEKSG
                     KVLQVGSQYPSMGCAREAAELIKSGRLGQVTLIEGRIHRNTATGAWYYPIPPDASPET
                     IDWKSFLGSAPPCDFDLRRFFQWRLFWDYSGGLPTDLFVHLITAVHQMMGVQEPESVV
                     SFGGIYHWKEYREVPDQMTALVRYPEGFVLQLTSTASNGHPAPILKIYGTAGTIEYNG
                     DSFTYFYEPRGEGFAYSTHSWPEATVAEFKRRMKLNDRLQPIDGPAAAAPVEYKAPND
                     EDSTRAHLRNWIDAIRTGGKPIEDARAGHHAALVGHMCNLSYKSGKPVTWNRKTRKVE
                     A"
     gene            complement(3007..3405)
                     /locus_tag="PKK95_06350"
     CDS             complement(3007..3405)
                     /locus_tag="PKK95_06350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOC17866.1"
                     /translation="MTSAPPTVVTAAVIERDGKLLVARRLEGTHLAGHWEFPGGKCEP
                     GEEIRACLVRELLEELGVSAAVGGEIHRTFYAYDDRRLELIFFECEIDGEPRPLLGQE
                     IRWVARADLRGLAFPPADAELIEQLGKGQS"
     gene            complement(3402..4124)
                     /locus_tag="PKK95_06355"
     CDS             complement(3402..4124)
                     /locus_tag="PKK95_06355"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOC17867.1"
                     /translation="MAEGGRTAAQRAGRRAGKGAELPAPSRRAAFRRRLLAWYRANGR
                     DLPWRRPADPYHVLVSEIMLQQTQVDRVLPKYAEWLAKYPSLEALAGAPEDEVVRTWY
                     PLGYNVRPRRLHSIAREAVERYGGRLPEDEPTLLGFKGIGRYTAGAIRSFAFGRRAAI
                     LDTNVARVLFRVFVGSGNPRDHATRRRLWAISEAMLPRKHVFDFNQGLMDLGAMVCAP
                     RKPRCAACPVRSLCVSREGLRS"
     gene            4238..4777
                     /locus_tag="PKK95_06360"
     CDS             4238..4777
                     /locus_tag="PKK95_06360"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOC17868.1"
                     /translation="MKIYTRTGDAGETALFDGRRVPKSDLRVEAYGDVDELNAVVGLV
                     LAEGLDEDLAAMVRAVQRDLFALGGRLADPARKIAGRVGKVAIGDPDIARLEEWIDAL
                     EMELTPLRHFILPGGTRAASLLHVCRTVCRRAERRVVQLGSEAVEPALLAYLNRLSDF
                     LFVLARAANRRAGVAETEW"
     gene            4771..5685
                     /gene="hpnC"
                     /locus_tag="PKK95_06365"
     CDS             4771..5685
                     /gene="hpnC"
                     /locus_tag="PKK95_06365"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOC17869.1"
                     /translation="MVTASPGLRLGEAYAACERLARRHYENFPVASRLVPPRMRPHVA
                     AVYAFARVADDFADEAVSTPAERLAALDAWGRRLEEAGGGGPPRSEPAPDLLPPETDA
                     IFAALGHTIAECALPVSLFEDLLSAFRQDVTKQRYGSWEEVLDYCRRSANPVGRIVLR
                     IAGCRDGQLDASSDALCTALQLTNFWQDLERDREKGRLYLPLDECAAARANLADLAAG
                     RLTPAWRTVLERAAALTASLFESGRLVCDRVPGRLRWELRLTWLGGRRILERLERDGF
                     DVFHRRPTLGAADAPLLAWRALAWRRAT"
     gene            5682..6518
                     /gene="hpnD"
                     /locus_tag="PKK95_06370"
     CDS             5682..6518
                     /gene="hpnD"
                     /locus_tag="PKK95_06370"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: protein motif:HMM:TIGR03465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HOC17870.1"
                     /translation="MSGRNTSFYYSFLALPTEKRAAIVAVWDFCRAVDDAVDEAAGAT
                     EAEVHAEGGRQLASWRRELAACFGAEEPCTPQGRALKRHVAPFGLPRDALEAVIDGVA
                     MDLERTRYATFEDLREYCLRVASAVGLVCIEIFSYRDPGARQYAVDLGIALQLTNIVR
                     DVATDLRRGRIYLPLDELAAHGCTEDDLRAGVVTGRVRALLAFQAARARDYYRRAEAS
                     LPRGDRRRLVAARIMGAIYEATLRRVERSGYDVFSGKVGLPRWRRAAIAALVWAKTMV
                     GG"
     gene            6518..7867
                     /gene="hpnE"
                     /locus_tag="PKK95_06375"
     CDS             6518..7867
                     /gene="hpnE"
                     /locus_tag="PKK95_06375"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HOC17871.1"
                     /translation="MRALPSPDVVVVGGGLAGLSAASALASRGARVLVLEARPTLGGR
                     ASSFTDPATGERVDNGQHLMLGCYSETFAFLERIGARSSVRVEDSLEVGMVDRDGQSS
                     VLRCPVLPSPLHLLAGLAEWDAVGWADRLSALAVARPLRRAQRFLKTGKGWLPASPGE
                     TVSAWLERNGQSARLREMLWEPLALAALNQDAGEAAAPVFLRVLAQMFGPDPRDSAIA
                     VPLLPLTELYAEPAREYIQARGGRVLTSALARVRISGGRVESVEAGGERCTAAAVVAA
                     VPWFALDDTLAGDTAPMSAVLASARAMRASPIVTANLWFDRPVMDVASVGLPGRAFQW
                     VFDKRRVFGDHASHLSCVSSGAAALVTRSNEELAAQARDELAAALPGAGGARMLRAVV
                     VRERRATFSLAPGEPARPSTRTEVRGLLLAGDWTGTGLPATIEGAVLSGHRAAEELY"
     gene            7889..9085
                     /gene="thiI"
                     /locus_tag="PKK95_06380"
     CDS             7889..9085
                     /gene="thiI"
                     /locus_tag="PKK95_06380"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOC17872.1"
                     /translation="MNSVVVHYQEVALKGKNRPWFISRLVANLRAAVADLDVKSVRPL
                     VGRIELTLGPAARYEEVRDRIRHVFGVANCSRAGRSGRGLDEMAEAILAGARDLDPES
                     FRVSVRRADKRYPLRSPEVEREVGGRIKLARGWKVNLDNPALTVGIEILNDHAFYFFG
                     KDKGPGGLPTGSGGRVACLLSGGIDSPVAAWRMMKRGCQALLVHFHSYPFLSRASQEK
                     VRELAALLTRYQLHSRLFLVAFGEIQRQVVLSVPAALRVVVYRRLMLRIAERIARDHG
                     AGALVTGEVIGQVASQTLENMSVIGSVASLPVLRPLVGMDKEEIVAQAERIGSYEISI
                     VPDQDCCQLFTPKHPATRARLEEVLAAESLLPVDDLVAGAAAAAEVEEFAFPAAAAGA
                     GKGARG"
     gene            9082..11412
                     /locus_tag="PKK95_06385"
     CDS             9082..11412
                     /locus_tag="PKK95_06385"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOC17873.1"
                     /translation="MKALEYWGVGAALLLALWGVAWQNYLLFHALVEFAAIAVAAAMF
                     AVAWNARRFSTNGFLTFLGISMLPVAGIDLFHTLSYRGMGVFGALGADRATQFWIAGR
                     ALEAAIFLVSPFFLTRRVGAWRTLLASTGIAVALALSIVPLGMFPTAYVDEGPRQGLT
                     PFKIAAEYVICLVLVAGGAFLWRRRDHLAEPTYRLVLSALVTKILSELAFTQYVSVYG
                     DFNKLGHLLKFVSSYLVFKAVVEGSLRTPYQVLFRDLQQQEETLQASERRYRSLVEMS
                     PDALYVNRDDRIEYANPAAVELFGGTSLEEIVGRSPYHFFHSEYHPKMRERIATLLEG
                     RPVPLVESRVVRLDGRIRDVEVVATPFDDVQGRAIQVILRDITERKRTREELRENARR
                     LEEASRMKDEFLATLSHELRTPLNAILGWSEILLQGSLDATHTRRALEVIARNARAQT
                     ALIGDVLDVSRIISGRLRLNLRDTDGVDALRAALDTVRPAAEARRIQVVAELPQNSPM
                     RADPDRLQQVFWNLLSNAVKFTPEGGRIDVSCRRDGDHAVFEVHDTGEGLDPAALEHL
                     FERFWQADRSTSRRHGGLGLGLAIVRHLVELHGGQIRADSEGPGRGATFQLSLPTRGA
                     AEAPLLDESGLTPAGEQRPTGVGGFDGLKVLVVDDDTDARDVAAASLQSLGIAVELAD
                     TSAAGLRIVSDWRPDVIVADIGMPGEDGYHFVRQLRALGADRGGLTPAIALTAYSRPE
                     DRRRAIEAGFGDHLPKPVLPHVLAAAIARAAGRTEA"
     gene            11482..>11684
                     /locus_tag="PKK95_06390"
     CDS             11482..>11684
                     /locus_tag="PKK95_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOC17874.1"
                     /translation="MSILKVARMGHPVLRERARRVEPAEIKTPAFQKLVDDMIETMRE
                     YDGIGLAAPQVHEKVRLAIIGIE"
BASE COUNT         1633 a         4065 c         4183 g         1803 t
ORIGIN      
        1 caccaaggag cggctcgaag cggagtacaa ggagtttgcc tcggcgtacc ggtgaatccc
       61 gatccctacc cctcggtgac cacttcctgt ttcgcgtcgt cgaacgtcac gcgccggccc
      121 gtctgcacgg cggcgatcgt catgcagagc gcgaccgagt ggctgtagcc cgcctcgatg
      181 ccggcgttgg gagtcttgcg gctgcggacg caccgcatcc agtcgagcat gttggcgagc
      241 gtcgcggtat ccacccccgt gtcggcggcg gtctcgaggg tggtggcgtt ctcgacgagc
      301 gtctgcccgg caagcaggtt gggcggcatt cccatcgcgg ccgcctcgcg ctctccaagg
      361 ccgcctgccg gcgtcaccag gttcttgtcc aggtcgagcg tgccgccgtt cgagtagtac
      421 agctccttcg tgcccccggc cgagttggtc atccgggacg agtagacgac ctggaatccc
      481 ttggaggcgt cgtcgagtgg gccgtagtcg aacaccgcgg tcatcgtgtc ccagttgcgg
      541 cggccgtcct tccagaggta gatgccgccg ttggcgacga cgctgcgcgg gcgcggcagc
      601 ttggtgaacc agtggacggt gtcgatctgg tgcacgagcc actggtcggg aatgcccgag
      661 gagtacggcc agaacaggcg gaactcgagg tacttccgcg ggtcgaacgg ctcgaagggg
      721 cggttcatca ggtagcgctt ccagtcggtg tcggcctcgc gaagctgctt gaccacagcc
      781 ggccgccgcc accgcccggg ctggttgacg ttccaggtca tctccaccga gacgatgtcg
      841 ccgaagcggc ccgactggat gaactcgcag gctcgctggt agaccggctc gctgcgccgc
      901 tgggtgccga tctggacgac gcggcccgtg tcctttactg cctgccggat cgcccgggcg
      961 tcctccatcg tgttggcgag cggcttctcg atgtacgcgt cgcgccccga gcgaaccgcc
     1021 tcgacgccgt gcagcgcgtg ctggaagtcg gccgtcgcga tgatcaccgc gtcgacgtcc
     1081 ttgcgggagt acagctcgtc gttgttgcgt accacgtccg gccggcgccc gcaaagcttc
     1141 tccacgtggg ccatcgcctg ttcgcggcgc aggctccaca ggtcggagac ggcgaccagc
     1201 tcgaaattct cggcggtggc gtgctggagg aacgcgggga tcagcgacga ccgcgcgcgg
     1261 tcggagaagc cgaccaccgc gacgcggacg cggtcgttgg cgccgaggat gctggcgtag
     1321 ctcgacgcgc tggaggcgcc ggccgccgcg aggcccaggc cggtggtctt gatgaaggtc
     1381 cgacgcgaca cgtgcgacat gacaggcacc tcctccttcg tttcctggcc cctgtctacg
     1441 ccgttgcggc tgtgactgtc aaggcggaag cgcgccgggt tgacttccac cgagagcgcg
     1501 tggtactaca cgcgccgtcc atcccgggcg cccggcgccc ctgcacggag gcttctgcat
     1561 gtcacgccac gattcgattg gccggagaca gttcctcgag gaaaccacgt tgctcgcagc
     1621 cgtggcctcc ggcctcgggg cagcccgccc cgcggcggcc cagatcaaca actcggctgc
     1681 cctgccggtg aagcccgtga gggtcgggtt cgtcggcgtc gggatccggg gcacgctgct
     1741 gatggaggcc gtgaagggga tcgccggcgc cgagatcgtc gccgccgccg actgctacaa
     1801 gggccacctc gatcgggcca aggagctgat ctcgccctcg ctccagacga ccggcgacta
     1861 tcggcaggtg gtcaccaggc ccgacgtgga cgccgtcgtg atcgcgacgc ccgaccactg
     1921 gcacctgcag atgacgcgcg aggcgctggc cgccggcaag cacgtctaca tcgagaagcc
     1981 catgacccac tcgtgggagg agggggaggc gttcatcgag gccgccgaga agagcggcaa
     2041 ggtgctgcag gtgggcagcc agtaccccag catgggatgc gcgcgcgagg ccgcggagct
     2101 gatcaagagc gggcgcctcg gccaggtgac gctcatcgag gggcgcatcc accgcaacac
     2161 ggcgaccggc gcctggtatt acccgattcc ccccgacgcc tcgcccgaga cgatcgactg
     2221 gaagagcttc ctcgggtccg ccccgccgtg cgacttcgac ctccgccgct tcttccagtg
     2281 gcggctgttc tgggactact ccggcggcct gccgaccgac ctcttcgtcc acctgatcac
     2341 cgccgtgcac cagatgatgg gggtccagga gccggagagc gtggtcagct tcggcggcat
     2401 ctaccactgg aaggagtacc gcgaggtgcc cgaccagatg accgcgctgg tgcgctatcc
     2461 cgaggggttc gtcctgcagc tgaccagcac cgccagcaac ggccacccgg ccccaatcct
     2521 caagatctac ggcaccgccg ggacgatcga gtacaacggc gactcgttca cctacttcta
     2581 cgagccgcgc ggcgagggct tcgcctactc cacccactcg tggcccgagg cgaccgtcgc
     2641 cgagttcaag cggcgcatga agctcaacga caggctgcag ccgatcgacg gcccggcggc
     2701 tgcggcgccc gtcgagtaca aggccccgaa cgacgaagac tcgacccgcg cgcacctgcg
     2761 caactggatc gacgcgatcc ggacgggggg gaagccgatc gaggacgccc gggccggcca
     2821 ccacgccgcg ctcgtcggcc acatgtgcaa cctctcctac aagtcgggca agccggtgac
     2881 gtggaacagg aagacgcgga aggtcgaagc gtaggagctg cgcagggctg gcaggttgct
     2941 gaaagaagaa gcctgcggcc tgctagagcc ctgcggttgc agggagcagc ccattcgggc
     3001 tgctcctcag gactgtcctt ttcccagctg ctcgatcagc tcggcgtcgg cgggggggaa
     3061 ggcgaggccg cgaagatcgg cgcgcgcgac ccaccgtatc tcctgcccga gcagcggccg
     3121 cggctcgccg tcgatttcgc actcgaagaa gatcagctcc agccgccggt cgtcgtaggc
     3181 gtagaacgtg cggtggatct cgccgcccac cgccgcgctg acgcccagct cttcgagcag
     3241 ctcgcgcacc aggcaggccc ggatctcctc accgggctcg cacttccccc cggggaactc
     3301 ccagtgaccc gccaggtgcg ttccctcgag acgccgtgcg acgagaagtt tcccgtcacg
     3361 ctcgatcacg gcggccgtta ccacggtcgg cggtgcggag gtcatgacct caggccttca
     3421 cgcgacacgc agagggagcg cacggggcag gcggcgcacc gcggcttgcg cggggcgcac
     3481 accatggcgc caaggtccat cagcccctgg ttgaagtcga agacgtgctt tcgcgggagc
     3541 atcgcctccg agattgccca gaggcgccgg cgggtggcgt gatcgcgagg gttgccgctg
     3601 cccacgaaca cgcgaaagag cacgcgggcg acgttggtgt cgaggatggc cgcccggcgg
     3661 ccgaacgcga aacttcggat cgcccctgcc gtgtagcgcc cgatcccctt gaaccccagc
     3721 agcgtcggct cgtcctccgg caggcgcccg ccgtaccgct ccaccgcttc gcgtgcgatc
     3781 gagtgcaggc gcctcggcct gacgttgtag cccaggggat accacgtgcg aaccacctcg
     3841 tcctcggggg ccccggccag cgcctcgagc gagggatact tggccagcca ctcggcgtac
     3901 ttcggcagca cccgatccac ctgcgtctgc tggagcatga tctcggagac gagcacgtgg
     3961 taggggtcgg cgggccggcg ccacggcagg tcacgcccgt tggcccgata ccaggcgagc
     4021 aggcgccgcc tgaaggccgc ccggcgtgag ggcgcgggga gttcggctcc ctttcccgcc
     4081 cgtctgcctg cccgctgcgc cgccgttcgc ccgccctccg ccatcaagct actaccttaa
     4141 ccgttcttcg gttccagccg gaaggcggtg ctgcggcctc gcgacgtctg gcctccgtct
     4201 tctgcctcgc cttctcttct caccgtataa ttcccgcgtg aagatctaca cccggacggg
     4261 tgacgccggc gagacggccc tgttcgacgg gcgccgggtt ccgaagtccg acctccgggt
     4321 cgaggcgtac ggcgatgtgg acgagctgaa cgccgttgtc ggcctggtgc tggccgaggg
     4381 gctggacgag gacctcgccg cgatggtgcg cgccgtgcag cgcgacttgt tcgcgctggg
     4441 cgggcggctg gccgaccctg ccaggaagat tgccggacgc gtggggaagg ttgccatcgg
     4501 cgacccggac atcgcccgcc tcgaggagtg gatcgacgcg ctggagatgg agctgacccc
     4561 gctgcgccac ttcatcctgc cgggcggcac gcgcgcggcc tcgctgctcc acgtctgccg
     4621 cacggtctgc cgccgagccg agaggcgcgt cgtgcagctc gggagtgagg ccgtcgagcc
     4681 cgcgctgctc gcctacctga accggctctc cgacttcctc ttcgtcctgg cgcgcgcggc
     4741 gaaccggcgc gccggcgtgg cggaaaccga atggtgacgg cgtctccggg cctccggctc
     4801 ggcgaggcgt acgccgcgtg cgaacgcctc gcgaggcgtc actacgaaaa cttcccggtg
     4861 gcgtcgcggc tggtgccgcc ccgcatgcgg ccgcacgtgg cggcggtgta cgccttcgcc
     4921 cgcgtggccg acgacttcgc ggacgaggcg gtctcgacac ccgcggaacg gctggccgcg
     4981 ctcgacgcct ggggaagaag gctggaggag gcgggcggag gcgggcctcc acggagtgag
     5041 cccgcgccgg acctcctgcc gcccgaaacc gacgccatct ttgccgcgct cgggcacacg
     5101 atcgctgaat gcgccctgcc ggtgtcgctc ttcgaggacc tgttgagcgc gttcaggcag
     5161 gacgtcacga agcagcggta cggttcgtgg gaggaagtgc tcgactactg ccgccgctcc
     5221 gcgaacccgg tcggccgcat cgtccttcgg attgccgggt gccgcgacgg gcagctcgac
     5281 gcctcgtccg acgccttgtg cacggccctg cagctgacca acttctggca ggacctcgag
     5341 cgcgaccgcg aaaaggggcg gctgtacctc ccgctcgacg agtgtgcggc ggcgcgcgcc
     5401 aacctggccg acctggccgc cgggcgcctc acgcccgcgt ggcggacggt gctcgaacgg
     5461 gcggccgccc tcaccgcttc gctcttcgag agtggacggc tggtgtgcga ccgcgtgccg
     5521 gggcgcctgc gctgggagct tcggttgacc tggcttggcg gccgaaggat tctcgagcgc
     5581 ctcgagcggg acggcttcga cgtgtttcac cgccgcccga cgctcggggc ggccgacgcg
     5641 ccgctgctgg cctggcgtgc gctggcctgg cgtcgtgcga catgagcggg cgcaacacca
     5701 gcttctacta ctcctttctg gcgctgccga cggagaagcg ggcggcgatc gtcgccgtct
     5761 gggacttctg ccgcgcggtg gacgacgccg tggacgaggc cgcgggggcg accgaggccg
     5821 aggtgcacgc cgagggtggg cgccagctgg cttcgtggcg gcgcgaactg gccgcctgct
     5881 tcggcgcgga ggagccgtgc acgccccagg gccgggcgct gaagcggcac gtcgccccgt
     5941 tcggcctgcc ccgcgacgcc ctcgaggccg tgatcgacgg cgtcgccatg gacctcgagc
     6001 gcacccgcta cgccaccttt gaagatctgc gcgagtactg cctcagggtc gcgtcggcgg
     6061 tcggcctcgt ctgcatcgag atcttctcgt accgcgatcc cggtgcgcgc cagtacgcgg
     6121 tggacctggg catcgccctg cagctgacca acattgtccg cgacgtggcg accgacctgc
     6181 gccgcgggcg gatctacctt ccgctcgacg agctggcggc gcacggctgc acggaggacg
     6241 acctgcgagc gggagtggtc accgggcggg tacgggcgct gctcgccttc caggcggcgc
     6301 gggcgcgaga ctactaccgg cgcgccgagg cctcgctgcc tcgcggcgac cggcggcgcc
     6361 tcgtcgccgc caggatcatg ggggccatct acgaggcaac gctccgccgg gtggaacgtt
     6421 cgggctacga cgtgttctcg gggaaggtgg ggctgccccg ctggcggcgt gcggcgattg
     6481 ccgccctcgt gtgggcgaag acgatggtgg gcgggtgatg cgggcgctgc cttcgcccga
     6541 cgtggtggtt gtcggcggcg ggttggccgg cctgagcgcc gcatcggccc tcgcgagtcg
     6601 cggcgcccgc gtgctggttc tcgaggccag gccgacgctg ggcggccgcg cctcgtcttt
     6661 cactgacccc gccacaggcg agcgcgtgga caacggccag cacctgatgc tcgggtgcta
     6721 ttcggagacc ttcgcgttcc tcgaacggat cggggcgagg tcgtcggtgc gcgtcgagga
     6781 ctccctcgag gtcgggatgg tggaccggga tggccagtcg agcgtgctcc gctgcccggt
     6841 gctgccctcg ccgctgcacc tgctggccgg cctggcggag tgggacgccg tcggctgggc
     6901 cgaccggctc tcggcgctgg cggtggcccg gccgctgcgc cgggcccagc gcttcctgaa
     6961 gaccggcaag gggtggctgc cggcgtcgcc cggcgagacg gtttcggcgt ggctcgaacg
     7021 caatgggcag agcgcccggt tgcgcgagat gctctgggag ccgctggcgc tggcggcgct
     7081 gaaccaggat gctggcgagg cggcggcccc cgtgttcctg cgcgtgctcg cacaaatgtt
     7141 cgggcccgac ccgcgcgact cggccatcgc ggtgccgctg ctgccgctca ccgagttgta
     7201 cgccgagccg gcgcgggagt acatccaggc gcgcggtggg cgcgtgctga cctccgcact
     7261 cgcgcgcgtg cgaatctcgg gcgggcgtgt cgagtcggtg gaggctggcg gagagcgctg
     7321 cacggcggcg gccgtcgtgg cggccgtgcc atggttcgcc ctcgacgaca cgctggccgg
     7381 cgacaccgcg ccgatgtcgg ccgttctcgc ctcggcgcgc gcgatgcgcg cgtcgcccat
     7441 cgtgacggcc aacctctggt tcgaccggcc ggtcatggac gtcgcatcgg tcggcctgcc
     7501 aggccgggcg ttccagtggg tgttcgacaa gcggcgggtg ttcggcgatc atgcctcgca
     7561 cctctcctgc gtgtcgagcg gcgctgccgc gctcgtcacg cgatcgaacg aggagctggc
     7621 ggcgcaggcg cgcgacgagc tggcggcggc gcttccagga gcgggcggcg cgagaatgct
     7681 ccgcgcggtg gtggtgcgcg agcggcgcgc caccttctcg ctggcgccgg gtgagccggc
     7741 acgcccctcc acccgtaccg aggtccgtgg tttgctgctc gccggcgact ggaccgggac
     7801 ggggctgcct gcgacaatcg agggggctgt actgagcggt catagagcgg cggaagagtt
     7861 gtactgaatt gatccctgat ccctgtccat gaactccgtc gtcgtccact accaggaagt
     7921 tgcgctgaag ggcaagaacc ggccgtggtt catttcgcgg ctggtcgcga acctccgcgc
     7981 cgcggtggcc gatctcgacg tgaagtcggt ccggccgctg gtggggcgca tcgagctgac
     8041 gctcggcccg gcggcgcggt acgaggaggt gcgggaccgg attcggcacg tcttcggcgt
     8101 cgccaactgc tcgcgggcgg gccgttcggg acggggcctg gacgagatgg cggaggcgat
     8161 cctggccggc gcgcgcgacc tcgaccccga gtcgttccgg gtctcggttc gccgggccga
     8221 caagcgctat ccgctgcgct ccccggaagt ggagcgcgag gtcggcggac gcatcaagct
     8281 cgcgcgcggg tggaaggtga acctcgacaa cccggcgctg acggtcggga tcgagattct
     8341 caacgatcac gccttctact tcttcggcaa ggacaaggga ccgggcggcc tgccgaccgg
     8401 gtcgggcggc cgggtggcgt gcctcctctc ggggggcatc gactcgccgg tggcggcgtg
     8461 gcggatgatg aagcgcggct gccaggcgct gctcgtccat tttcacagct acccgttcct
     8521 gtcgcgcgcg tcccaggaga aggtgcgcga gctggccgcg cttctcacgc gctaccagct
     8581 gcacagccgc ctgttcctcg tcgccttcgg cgagatccag cgccaggtgg tgctgagcgt
     8641 gccggccgcc ctccgcgtgg tcgtgtaccg gcgcctcatg ctccgcattg ccgagcgcat
     8701 cgcgagggac cacggcgcgg gggcgctggt gaccggcgag gtgatcggcc aggtggcctc
     8761 gcagaccctc gagaacatgt cggtgatcgg ttcggtggcc tcgctgccgg tgctgaggcc
     8821 gctcgtgggc atggacaagg aggagatcgt cgcgcaggcg gagcggatcg gctcctacga
     8881 gatctccatc gtgcccgacc aggattgctg ccagctcttc accccgaagc acccggccac
     8941 gcgcgcccgc ctggaggagg tgctggccgc tgagagcctg ctgcctgtcg acgacctggt
     9001 ggccggggcc gcggcggccg ccgaagtgga ggagttcgcg ttcccggccg cggccgccgg
     9061 cgcgggcaaa ggcgcgcggg gatgaaggcg ctcgagtact ggggcgtggg cgcggccctc
     9121 ctgctggccc tgtggggcgt ggcctggcag aactacctgc tgttccacgc gctcgtcgag
     9181 ttcgccgcca tcgcggtggc ggcggcgatg ttcgccgtcg cctggaacgc ccgccgcttc
     9241 tcgaccaacg gcttcctgac cttcctcggc atctcgatgc tgccggtggc gggcatcgac
     9301 ctcttccaca ccctctccta cagggggatg ggggtgttcg gagcgctcgg tgccgaccgg
     9361 gcgacccagt tctggatcgc cggcagggcg ctcgaggccg ccatcttcct cgtctcgcct
     9421 ttcttcctca cgcggcgagt gggtgcgtgg aggacgctgc tcgcgtcgac cggcatcgcg
     9481 gtggcgctgg ccctgtcgat cgtcccgctg ggcatgttcc cgacggccta cgtggacgaa
     9541 gggccccggc agggccttac gccgttcaag atcgccgccg aatacgtcat ctgcctcgtc
     9601 ctggtcgccg gcggtgcctt cctgtggagg cgccgcgacc accttgccga gccgacctac
     9661 aggctggtcc tgtcggcgct cgtgaccaag atcctctccg agctcgcctt cacgcagtac
     9721 gtgagcgttt acggcgactt caacaagctc ggccacctcc tcaagttcgt ctcgtcgtac
     9781 ctcgtgttca aggcggtcgt cgaggggagc ctccggacgc cgtaccaggt tctcttccgc
     9841 gacctgcagc agcaggagga gacgctccag gcaagcgagc ggcggtaccg gtcgctggtg
     9901 gaaatgtcgc ccgacgcgct gtacgtgaac cgcgacgacc ggatcgagta cgccaacccg
     9961 gccgcggtcg agctgttcgg cggcacctcg ctggaagaga tcgtcgggcg ctctccttac
    10021 cacttcttcc actccgagta ccaccccaag atgcgcgagc ggatcgccac gctgctcgag
    10081 ggccggccgg tgccgctcgt ggagagccgc gtcgtacgtc tggacggccg gatccgcgac
    10141 gtcgaggtgg tggccacccc cttcgacgac gtccagggca gggcgatcca ggtgatcctg
    10201 cgggacatca ccgagcggaa gcgcacacgc gaggaactgc gcgagaacgc gcggcggctc
    10261 gaggaagcca gccgcatgaa ggacgagttc ctggccacgc tctcgcacga gcttcggacc
    10321 ccgctcaacg ccatcctcgg gtggtcggag atcctgctcc agggaagcct cgacgccacg
    10381 cacacgcgcc gcgcgctgga ggtgatcgcg cgcaacgcca gggcccagac ggcgctcatc
    10441 ggcgacgtgc tcgacgtctc acgaatcatc agcggccggc tgcggctgaa cctgcgcgac
    10501 accgacggag tcgacgccct ccgcgcggcc ctcgacacgg tgaggcccgc ggccgaggcg
    10561 cgccgcatcc aggtggtcgc cgagctgccc caaaactcgc ccatgcgcgc agacccagac
    10621 cgcctgcagc aggtgttctg gaacctgctg tcgaacgccg tgaagttcac gcccgagggg
    10681 gggcggatcg acgtctcgtg ccgccgcgac ggcgaccacg ccgtattcga ggtgcacgac
    10741 accggcgagg ggctcgatcc ggcggcgctc gagcacctct tcgagcgttt ctggcaggcc
    10801 gaccgctcga cctcgcgccg ccacggcggc ctcggtctcg gcctggccat cgtcaggcac
    10861 ctcgtcgagc tgcacggcgg acaaatccgc gccgacagcg aggggccggg gcgaggcgcc
    10921 accttccagt tgtcgcttcc cacccgcggc gctgcggagg cgccgctcct cgatgagagc
    10981 ggcttgacgc cggccggcga gcagcggccc acgggcgtgg gtgggttcga cggtctcaag
    11041 gtgctcgtcg tggacgacga caccgacgct cgcgacgtgg cggccgcatc gctgcagagc
    11101 cttggcatcg ccgtcgagct cgccgacacc tcggccgccg gactccgaat cgtctccgac
    11161 tggcgtccgg acgtgatcgt cgccgacatc ggaatgccgg gtgaggacgg ctaccacttc
    11221 gtgcggcagt tgcgcgcact cggagccgac cggggcggcc tgacgccggc catcgccttg
    11281 accgcgtaca gccggcccga ggatcggcgg cgggcgatcg aggccgggtt cggcgaccac
    11341 ctcccgaagc cggtgttacc gcacgtgctc gcggctgcca tcgcgcgcgc cgccgggcga
    11401 acagaagcgt agggcgcacc cggcgccgga gcggtgaccc gcccgttccc ccgcgtccgg
    11461 ggcccgatat aatggttcga tatgtcgatc ctcaaggtcg cacgcatggg gcaccccgtg
    11521 ctgcgagagc gcgcccgccg tgtcgagcca gccgagatca agacgcccgc cttccagaag
    11581 ctggtggacg acatgatcga gacgatgcgc gagtacgacg ggattggcct cgccgcgccg
    11641 caggtgcatg agaaggttcg cctcgcgatc atcgggatcg agga
//
LOCUS       DAOPQX010000082        11681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6356_length_11681_cov_5.182006, whole genome shotgun sequence.
ACCESSION   DAOPQX010000082 DAOPQX010000000
VERSION     DAOPQX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11681
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6356_length_11681_cov_5.182006"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKK95_06395"
     CDS             complement(<1..403)
                     /locus_tag="PKK95_06395"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HOC17875.1"
                     /translation="MIANDFLSLRDFSPEQVRHLLDLAADVKADRRKFSKALEGQTLA
                     MIFEKPSLRTRVTFDVGIQQLGGFSLYLSPAEINLGKRESIHDVAKNLERMVQGIMIR
                     TFAHSIVEEMARHASVPIINGLTDYSHPCQAM"
     gene            complement(559..1833)
                     /gene="pcnB"
                     /locus_tag="PKK95_06400"
     CDS             complement(559..1833)
                     /gene="pcnB"
                     /locus_tag="PKK95_06400"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOC17876.1"
                     /translation="MVAPTIIQRAEHPISRRNIDPDALKVLYRLKEGGFIAYLVGGGV
                     RDLLLARQPKDFDVGTSAHPYQVKRLFRNCWIIGRRFRLAHVKFGQKIIEVATFRRHV
                     PPSNGGDPGAADAADGGRDTFGGRDLLIHRDNTFGTPEEDAFRRDFTVNALFYDIATF
                     SIIDYVGGLRDLHDRVIRAIGDPEERFLEDPVRMLRAVAFAERLDFRLDPPVEDAIRL
                     LGSEIARSAPARLMEEYYKVLRTGSSARVFRALARVGLLGPVAPALQEAADSPALAAS
                     LERLDAHRRLFAATPDAFTNPVLLGSLLVPLGFTVENGHPSRQPARDAWLGLLPVARR
                     DLERLYQLLALQRRLRDPNLSPRAARALLSRGAFADALAWLEIHGSDPAAVSRWRALI
                     EELRSHETLPAPPAFIPVRRRRRRSRRPVVKS"
     gene            1852..2706
                     /locus_tag="PKK95_06405"
     CDS             1852..2706
                     /locus_tag="PKK95_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17877.1"
                     /translation="MLRDQRTQVRSLQWVRACTGLAAAIVALAAAPAAAQVGLDPARR
                     LYNEGRYAEAIELAARLRSRADLADAARLIIGRAQLERYRQTADRADLVAAREALREV
                     RGATLPPREQAELLVGLAESLYLEELYGSAADLFESAFEMAYLDSPRAFDRVFDWWAT
                     SLDRQAQSGVADDRDALYERIQLQAKDALMRDPGSAPAAYWQVVAYRALADPGRAWEA
                     AIAGWLRASLAGDRAAALRADLDRLVLQAVIPERVRQLADNDADRERAASQLREVWEG
                     IKKEWTPR"
     gene            complement(2749..5418)
                     /gene="polA"
                     /locus_tag="PKK95_06410"
     CDS             complement(2749..5418)
                     /gene="polA"
                     /locus_tag="PKK95_06410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOC17878.1"
                     /translation="MLFLVDGNSQMYRAYHAIRGLTGPDGRSTNAVYGFVTMLRKLVA
                     DQRPELIACTFDLAGPTFRSTLAADYKANRPAMPGDLAEQIPWVHQACEAMGVPIVSC
                     PGFEADDVIATIAERAKAEGYDVAIVTGDKDLFQLVGPGVRVYNPRDEGEWFDAEAVR
                     ERFGVPPERVVDVLALTGDAIDNIQGVPGIGDKGARSLIAAHGSLDALLEHAAEVPQK
                     KYREALLQHADAARQSRELARLRTDAPCAFEIERFRYRGPDRAACFAIFSELGFRTLL
                     AEYAPTAETVERDYRLVDSREALAELAGELRRAGAFALRVITSPGPAVQADLVGLAVA
                     TAPRGARYIPVGHHALDSGPQIVPGEALAALRPLLEDETVGKVGHDLKADAVALARHG
                     VTLRGLRFDTMLASYLLDATRSSHPLEVSSLEHLGYRAVTEESVCGRGARALALGDLP
                     PAAVLAYAGERADLPLQLADRFWALLQSEGLEPVYRELELPLIPVLAALERAGVKLDG
                     AVLAGLSGRVERELNERSGRIFELAGESFNINSPRQLSEILFDKLQLPSSKRTGKTRV
                     ASTAVEVLEELALAHELPRLILEWRGLQKLKGTYIDALPELVNPETGRVHTCFNQAVA
                     ATGRLSSSDPNLQNIPIRTELGREIRAAFVAEPGHVLISADYSQIELRVLAHLSGDEA
                     LTEAFRRGEDIHDRTARAVFGADSLLDRHELRRRAKVINYALLYGKTAYTLAKDIGVT
                     QQAAQAFIDAYFAGFPGVRRFIDALLTEARETGYVKTLFGRRRAVPELTSRNGQVRAA
                     AERVAVNLPIQGTAADILKKAMIALHGELDRRGRRARMILTVHDELVFEAPRVEADEV
                     AALVREGMEKAAPLAVPLTVEVGVGENWREAKN"
     gene            5500..6288
                     /locus_tag="PKK95_06415"
     CDS             5500..6288
                     /locus_tag="PKK95_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17879.1"
                     /translation="MVKVRAGRAARASLAALAVVLAAASCGGGSGLARQYEYDEDVHL
                     SLDGSATIYVNGSLPALVALRGFDLDLRPNARFDRERVNRAFSSPVTHVTRISNSRRL
                     GRRFAHLRIEVDDIRKLPGTAAFGWLSVAFDRAGDQFHYRATLGEAANRKVGDVGWKG
                     NELVAVRLHLPSKITYHNAGVNNLRRGNILVWEQSFADRLAGRPLEMEARMEPTSILY
                     HTLLLFAGSMLGAFTVLAAIVYWVVKRGKGRGAGGRGAVVPPAA"
     gene            complement(6293..6895)
                     /locus_tag="PKK95_06420"
     CDS             complement(6293..6895)
                     /locus_tag="PKK95_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC17880.1"
                     /translation="MPRPVFGAKPAVLRCARCGRGVDWEEARLQAVCGCRPHLDLPAV
                     LIRQAGPEDAAVMEAIFRRHYGRTALLAFGEALSLENCPAIVAELKGEVAGALAYRLQ
                     PRALHVVALATDPLWQRSGVASQLLAEAEVLAARHGLNKLVASTSNDNLPSLYLFQRR
                     GWLIDEVVPGALLSHAAALGGRGFGGIPVRDEVRLAKQVG"
     gene            complement(6960..8027)
                     /locus_tag="PKK95_06425"
     CDS             complement(6960..8027)
                     /locus_tag="PKK95_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC17881.1"
                     /translation="MSRIVGRRFVPALLLVLVAFVAGPAARQGSQRPLFRGGVDLVNV
                     SATVTDADGHFVKGLAKDDFLLLEDGVELPIAQFSSERVPVSLGIVVDASSSMDGERM
                     SQARRALEQLLDRLPDPDDEVFLYSFSEDPVLEHGWTTDRRAIRSALKRIDPGGRTSL
                     YDAVGRAVSLANTGRHAKKALVIISDGNDTASHTSVEALQRQIQESELLVYAIGVDAR
                     SLFQPKSRRSWFSQARPGFPRPPVIQGFPPRPGGGGGRPPVLPRPPDRAPAPPPVFDD
                     PVDLAALRSLTDDSGGRTEIVRTAGDLGPATSGIADELNRQYFLAYASNAPRDGKWHT
                     IELSVRDREWTVRARRGFIAQ"
     gene            complement(8159..8740)
                     /locus_tag="PKK95_06430"
     CDS             complement(8159..8740)
                     /locus_tag="PKK95_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC17882.1"
                     /translation="MRIFRKGPGPHALPLAVVGLRLGNRFLDVGARQGALFAEMAGKV
                     GLTGHACAVVGSEPDAVRVKAAAARAGVLADVEVTAWPGLPVGDASFDVAVVDNTDGL
                     LASLGETVRAHLAEEVLRALRPGGRALVLDRQPRGVLAALAGRGAASWPPAASVAAWL
                     TSAGFRPVRLLAEREGQRFTEGWKPAGPQGSAG"
     gene            8768..10081
                     /locus_tag="PKK95_06435"
     CDS             8768..10081
                     /locus_tag="PKK95_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOC17883.1"
                     /translation="MKQPVEITQPTGKLGILLVGLGAVSTTFIAGVMAVRKGIASPIG
                     SLTQMGTIRLGRRTEGRSPLVKDLVPLASLEDLVFGGWDIFEDDSYSAAHTAGVLDES
                     LLEQIRPELEAITPWPAVFDRRYVKRLDGPNVKRGSNKMELAQQVREDIRRFKAENGL
                     ARLVMIWCGSTEIFMTPSPAHQSIEAFEAGLCANDLTIPSSMVYAYAALKEGIPFANG
                     APNLTVDVPALMELAQQQGCPVAGKDFKTGQTLIKTVIAPGLKARLIGVRGWYSTNIL
                     GNRDGEVLDDPESFKTKEESKKSVLDYILQPDLYPQLYKDLCHVVRINYYPPRGDNKE
                     GWDNIDLVGWLGYPMQLKINFLCRDSILAAPIVLDLVLFLDLAARAGWSGIQEWLSFY
                     FKSPMHAPGLYPEHDLFIQLMKLKNTLRYLKGEELITHLGREYYD"
     gene            10139..10666
                     /locus_tag="PKK95_06440"
     CDS             10139..10666
                     /locus_tag="PKK95_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17884.1"
                     /translation="MPSSRMQPALFGGLVLGVLSALPVVSAGNVCCCLWLIAGGVVAA
                     YVLQSNQATPITTGDGAIAGLLAGIVGSFVYLVLSVPISLLTGPLQNRWVEKVLESGG
                     EVPEAMRPALEAMRQGGLSVLGVVFGFVMMLVLSLLFSTAGGAIGAAIFRKKTPPPPP
                     ATEYGAPSPPPPAGM"
     gene            10765..11451
                     /locus_tag="PKK95_06445"
     CDS             10765..11451
                     /locus_tag="PKK95_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC17885.1"
                     /translation="MALIETHDLWKSYTMGSEQIHALSGVSLAIERGEYVAIMGPSGS
                     GKSTLMNLIGCLDTPSRGSYLLNGKQVSQMNDDELARIRNEEIGFVFQTFNLLPRATA
                     LHNVELPLVYAGVPSADRLKRARAALERVELGDRVSHRPNQLSGGQRQRVAIARALVN
                     NPSILLADEPTGNLDSKTGEEIMALFERLHHAGNTIVLVTHEADIAAHAHRVLHIRDG
                     KIEDDVRRAA"
     gene            complement(11477..>11681)
                     /locus_tag="PKK95_06450"
     CDS             complement(11477..>11681)
                     /locus_tag="PKK95_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HOC17886.1"
                     /translation="LNLLMDLRDRLGLTYLFIAHDLRVVRHLCTHVAVMHHGRIVEAG
                     TADAIFGDPQDECTKRLLRCQST"
BASE COUNT         1788 a         4034 c         4208 g         1651 t
ORIGIN      
        1 ccatggcctg gcacggatgg ctgtagtcgg tcaggccgtt gatgatcggc acgctcgcgt
       61 ggcgcgccat ctcctcgacg atggagtggg cgaaggtgcg gatcatgatc ccctgcacca
      121 tccgctcgag gttcttcgcc acgtcgtgga tcgactcgcg cttgcccagg ttgatctcgg
      181 ccggcgagag gtagagcgag aacccgccca gctgctggat gccgacgtcg aaggtcaccc
      241 gtgtccgcag cgacggcttc tcgaagatca tcgccagcgt ctggccctcg agcgccttcg
      301 agaacttgcg gcggtcggcc ttgacgtcgg cggcgaggtc gagcaggtgg cgcacctgtt
      361 cgggggagaa gtcgcgcagg gacaggaaat cgttggcgat catgacagct ccagggaaca
      421 taccgccgga cgcgccgaaa ggggcgaaaa cagggccatt agagcccagc ggctcgaaga
      481 gggtcaagcc ggcggaaggt tatgcacgcg cctgcataac aggcagcgca tcggttttcg
      541 ctggtcggtc ttcggtcttc aggacttgac cacgggccgc cgcgagcgcc ggcggcggcg
      601 gcggacgggg atgaaggcgg gaggggccgg aagcgtttcg tggctgcgaa gctcctcgat
      661 caacgcgcgc cagcgcgaga cggcggcggg gtcggagccg tggatctcca gccacgccag
      721 cgcgtcggcg aacgcgccgc gcgagaggag cgcgcgcgcc gcgcggggcg agaggttggg
      781 gtcgcgcagg cgccgctgca gcgcgagcag ttggtagagg cgctcgaggt cacggcgcgc
      841 gaccggcagc aggccgagcc acgcatccct ggccggctgg cgcgaggggt ggccgttctc
      901 gaccgtgaag ccgaggggca cgagcagcga gccgaggagc acggggttgg tgaacgcgtc
      961 gggcgtggcg gcaaagagcc ggcggtgcgc gtcgaggcgc tcgagcgacg ccgccagcgc
     1021 gggcgagtcg gccgcctcct gcagggcggg cgcgaccggg ccgagcaggc cgacgcgggc
     1081 gagcgcgcgg aacacgcggg ccgacgaccc ggtgcggagc accttgtagt actcctccat
     1141 caggcgcgcg ggcgcgctgc gcgcgatctc gctgccgagc agccgaatcg cgtcctcgac
     1201 cggcgggtcg agccggaagt cgagacgctc ggcgaaggcg acggcgcgca gcatgcggac
     1261 cgggtcctcg aggaagcgct cctcgggatc gccaatcgcc cggatcaccc gatcgtgcag
     1321 gtcgcgcagc ccgcccacgt agtcgataat ggagaacgtc gcgatatcgt agaagagcgc
     1381 gttgacggtg aagtcgcggc ggaaggcgtc ctcttcgggc gtgccgaagg tgttgtcgcg
     1441 gtggatcagc aggtcgcgcc cgccaaaggt gtcgcgcccc ccgtccgcgg cgtcggccgc
     1501 gcccgggtcg cccccgttcg acggcggcac gtggcggcgg aaggtcgcca cctcgatgat
     1561 cttctgaccg aacttcacgt gggcgaggcg aaagcggcgg ccgatgatcc agcagttgcg
     1621 gaagagacgt ttgacctggt acgggtgcgc cgaagtgccg acgtcgaagt ccttcggctg
     1681 ccgggcgagc aggagatcgc gcacgccccc gccgaccagg tacgcgatga aaccgccctc
     1741 cttcaggcgg tagagcacct tcagggcgtc gggatcgatg ttgcgccgcg agatgggatg
     1801 ctcggcgcgt tgaatgatgg tcggcgcgac catggcggga gattatacca aatgctccgc
     1861 gatcagcgca cgcaagtccg ttcccttcag tgggttaggg catgcaccgg actggccgcc
     1921 gccatcgtgg cgctcgccgc ggcccctgcc gccgcccagg tcggcctcga cccggcgcgc
     1981 cgtctctaca acgaagggcg atacgcggag gcgatcgagc tggccgcccg gctacgctcc
     2041 cgcgccgacc tggcggacgc cgcgaggctg atcatcgggc gcgcgcagct cgagcgttat
     2101 cgccagacgg ccgaccgcgc cgacctggtg gccgcgcgcg aagcgttgcg cgaggtgcga
     2161 ggggcgacgc tgccgccgcg cgagcaggcg gaactgctgg tcgggctcgc cgagtcgttg
     2221 tacctcgagg agctgtacgg ctccgccgcc gacttgttcg agagcgcctt cgagatggcc
     2281 tacctcgact cgccccgggc cttcgaccgc gtcttcgact ggtgggccac ctcgctcgac
     2341 cggcaggcgc agtcgggcgt cgccgacgac cgcgacgcgc tgtacgagcg catccagctg
     2401 caggcgaagg acgcgttgat gcgcgacccg gggagtgccc cggcagccta ctggcaggtg
     2461 gtggcgtacc gcgcgcttgc cgaccccggc cgcgcctggg aggcggccat cgcgggatgg
     2521 ctgcgggcct cgctggcggg cgaccgcgcg gcagcgctcc gggccgacct cgatcggctg
     2581 gtgctccagg ccgtgattcc cgagcgcgtg cggcagctgg ccgacaacga cgccgaccgc
     2641 gagcgcgccg cctcccagct gagggaggtg tgggagggca tcaagaagga gtggacccca
     2701 cgttgaggca cccagggcag aaggaagaat gcattctgcc ttgcacggtc agttcttcgc
     2761 ctcccgccag ttctccccga cgccaacctc cacggtgagc ggcacggcga ggggcgcggc
     2821 cttctccatc ccctcgcgca ccagcgcggc cacctcgtcg gcctccacgc gcggcgcctc
     2881 gaacaccagc tcgtcgtgca cggtgaggat catccgcgcg cggcggccgc ggcggtcgag
     2941 ctcgccgtgc agcgcgatca tcgccttctt caggatgtcg gcggcggtgc cctggatggg
     3001 caggttcacc gctacccgct cggccgccgc cctcacctgc ccgttgcggc tcgtcagctc
     3061 gggcaccgcc cgccgccggc cgaacagcgt cttcacgtag ccggtctcgc gcgcctcggt
     3121 cagcagggcg tcgatgaagc gccgcacgcc cgggaagccg gcgaagtagg cgtcgatgaa
     3181 cgcctgcgcc gcctgctgcg tcaccccgat gtccttggca agcgtgtagg cggtcttgcc
     3241 gtacagcagg gcgtagttga tcaccttcgc gcgccggcgc agctcgtgcc ggtcgagcag
     3301 gctgtcggcg ccgaataccg cgcgggccgt gcggtcgtga atgtcctcgc cgcgccggaa
     3361 cgcctcggtg agcgcctcgt cgccggagag atgggccagc acacgaagct cgatctgcga
     3421 gtagtcggcc gagatcagca cgtgtcccgg ctcggcgacg aaggcggccc ggatctcgcg
     3481 cccgagctcg gtacggatcg gaatgttctg caggttgggg tcgctgctgc tcaggcggcc
     3541 cgtcgcggcc accgcctggt tgaagcaggt gtgcacccgc ccggtctcgg ggttcaccag
     3601 ctcgggcagc gcgtcgatgt aggtcccctt cagcttctgc aggccgcgcc actcgaggat
     3661 cagccgcggc agctcgtgcg ccaaagccag ctcctcgagc acctcgaccg cggtcgaggc
     3721 gacgcgggtc ttgccggtgc gcttcgacga ggggagctgc agcttgtcga acaggatctc
     3781 cgagagctgg cggggggagt tgatgttgaa cgactcgccg gccagctcga agatgcggcc
     3841 gctccgctcg ttcagctcgc gctcgacccg gcccgacagc ccggcgagca ccgcgccgtc
     3901 gagcttgacg ccggcccgct cgagcgccgc cagcaccggg atcagcggca gctcgagctc
     3961 ccggtacacc ggttcgagcc cctcggactg cagcagcgcc cagaagcggt ccgccagctg
     4021 gagcggcagg tcggctcgct cgcctgcgta ggcgaggacc gcggcgggcg ggagatcgcc
     4081 gagcgcgagc gcccgggcgc cgcggccgca cacgctctcc tcggtgaccg cgcggtatcc
     4141 gaggtgctcg agcgagctga cctccagcgg gtgcgacgac cgggtggcgt cgagcaggta
     4201 gctggcgagc atcgtgtcga agcgcagccc ccgcaaggtg acgccgtgcc gcgccagcgc
     4261 caccgcgtcg gccttcaggt cgtggcccac cttgccaacc gtttcgtctt cgagcagcgg
     4321 ccgcagcgcg gcgagcgcct ccccgggaac gatctgcggg ccgctgtcga gggcgtggtg
     4381 gccgaccggg atgtaccgcg cgccgcgggg ggcggtggcc accgcgaggc ccaccaggtc
     4441 ggcctgcacg gccggcccgg gcgaggtgat gacgcgaagc gcgaaggcgc ccgcgcggcg
     4501 gagctcgccg gccagctcgg cgagcgcctc gcgcgagtcc accaggcggt aatcgcgctc
     4561 cacggtctcg gccgtcggcg cgtactcggc cagcagggtg cggaagccca gctcggagaa
     4621 gatggcgaag caggcggcgc ggtcggggcc gcggtagcgg aagcgctcga tctcgaaggc
     4681 gcaaggcgcg tcggttctca gccgcgccag ctcgcggctc tgccgggcgg cgtcggcgtg
     4741 ctggagcagc gcctcccggt acttcttctg gggcacctcc gccgcgtgtt cgagcagcgc
     4801 gtcgagcgac ccgtgggcgg cgatcaggct acgggcgccc ttgtcgccga tccccggcac
     4861 cccctggatg ttgtcgatgg cgtcgccggt gagcgcgagg acgtcgacca cccgctcggg
     4921 cggcacgccg aaccgctccc tcaccgcctc ggcgtcgaac cactccccct cgtcgcgggg
     4981 gttgtagacg cggacgccgg ggccgaccag ctggaagaga tccttgtcgc ccgtcacgat
     5041 cgccacgtcg tagccctcgg ccttcgcccg ctccgcgatg gtcgcgatga cgtcgtcggc
     5101 ctcgaaccca gggcaggaga cgatggggac ccccatggcc tcgcacgcct ggtgaaccca
     5161 cgggatctgc tcggcaaggt cgccgggcat ggcgggccgg ttcgccttgt agtcggcggc
     5221 gagggtcgag cggaaggtgg ggccggcgag gtcgaacgtg caggcgatca gctcggggcg
     5281 ctgatccgcc accagcttcc gcagcatggt gacgaagccg tagacggcgt tggtcgaacg
     5341 gccatcgggc cccgtcagcc cgcggatcgc gtggtacgcc cggtacatct gcgagttgcc
     5401 gtcgaccagg aagagcaagg gggaagcgga cgatcgggag ttgttcatgg aaggcgccgt
     5461 gcgcgcgtgg cgggtgaagt cagtatatca ttggccgtca tggtgaaggt tcgcgccggg
     5521 cgagccgcgc gggcctcgct cgcggcgctg gcggtcgtgc tcgccgccgc gtcgtgcggc
     5581 ggcggcagcg gcctcgcacg gcagtacgag tacgacgagg acgtccatct ctcgctggac
     5641 ggttcggcca ccatctacgt caacgggtcg ctgccggcgc tcgtcgccct ccgcgggttc
     5701 gatctcgacc tccgccccaa tgcgcggttc gatcgcgagc gcgtgaaccg cgccttctcc
     5761 agcccggtca cccacgtgac gcgcatctcg aactcgcgtc gcctgggacg ccgcttcgcg
     5821 cacctgcgga tcgaggtgga cgacatccgg aagctgccgg gaaccgcggc gttcgggtgg
     5881 ctgtcggtgg cgttcgatcg tgcaggcgac cagttccact accgggcgac gcttggcgag
     5941 gccgccaacc gaaaggtggg cgacgtcggc tggaagggga acgagctggt cgcggtccgg
     6001 ctgcacctgc cgagcaagat cacgtatcac aacgccggcg tcaacaacct gcggcgcggc
     6061 aacatcctcg tctgggagca gtcgttcgcc gatcggctgg ccgggcgccc gctcgagatg
     6121 gaggcacgga tggagcccac ctccatcctc taccacacgc tgctcctgtt cgccggcagc
     6181 atgctcggcg ccttcacggt gctggcggcg atcgtctact gggtggtcaa aaggggaaag
     6241 gggcgcggcg ccggcggacg gggcgccgtg gtcccgcccg cggcctgatc gcctacccga
     6301 cctgcttggc cagccgcacc tcgtcgcgca ccgggatgcc gccgaacccg cgcccgccga
     6361 gggcggcggc gtgggacagc agcgcgccgg gcaccacctc gtcgatcagc cagccgcgcc
     6421 gctggaagag gtacagcgac gggaggttgt cgttgctcgt cgaggcaacc agcttgttca
     6481 ggccgtgccg cgccgcgagc acttccgcct ctgcgagcag ctgcgaggcg acgcccgagc
     6541 gctgccacaa cgggtcggtg gcgagggcga ccacgtgcag ggcgcggggc tgcaggcggt
     6601 aggccagcgc gccagccact tcgcccttca gctccgcgac aatggcgggg cagttctcca
     6661 ggctcaacgc ctcgccgaag gccaggaggg cggtgcggcc gtagtggcgc cggaagatcg
     6721 cctccatcac cgccgcgtcc tcgggcccgg cctgccggat caacacggcc gggaggtcga
     6781 ggtgcggccg gcacccgcac accgcctgga ggcgcgcctc ctcccagtcg acgccgcggc
     6841 cgcagcgggc gcatcgcagg acggccggct tcgcgccgaa cacgggtctg ggcataggac
     6901 ccctactgta gtgaaagcgt cgggggcggg caaggtcccc gcgggccacc gccggcgtgt
     6961 cactgcgcga tgaagccgcg gcgtgctcgg acggtccact cccggtccct cacgctcagt
     7021 tcgatcgtgt gccacttgcc gtcgcgcggc gcgttgctcg cgtaggcgag aaagtactgg
     7081 cggttcagct cgtcggcaat tcccgaggtc gccggcccga ggtcgccggc cgtcctcacg
     7141 atctcggtgc ggccgccgct gtcgtcggtg agcgagcgga gggcagcgag atccacgggg
     7201 tcgtcgaaca ccggcggcgg ggccggtgcc cggtcggggg gcctcggaag gactggcggc
     7261 cggccgccgc cgccgccggg cctgggcggg aagccctgga tgaccggcgg acgggggaac
     7321 ccgggccggg cctgcgagaa ccacgaacgg cggctcttcg gctggaacaa cgagcgggcg
     7381 tccacgccga tcgcgtacac gagcagctcc gactcctgga tctgccgctg cagcgcctcg
     7441 accgaggtgt gactcgcggt gtcgttcccg tccgagatga tgacgagggc tttcttggcg
     7501 tggcgtcccg tgttggccag cgagacggcc cgcccgacgg cgtcgtagag agacgtccgg
     7561 ccgcccggat cgatgcgctt gagcgccgag cggatggcgc ggcggtcggt cgtccagccg
     7621 tgctcgagca ccggatcctc gctgaacgag tacaggaaga cctcgtcgtc gggatccgga
     7681 agccggtcga ggagctgctc gagcgcgcgg cgcgcctgcg acatccgctc gccgtccatc
     7741 gaactgctcg cgtcgacgac gatcccgagg ctgaccggca cgcgctcgct cgagaactgc
     7801 gcgatgggca gctcgacgcc gtcctcgagc aggaggaagt cgtctttcgc aaggcccttg
     7861 acgaagtggc cgtccgcgtc ggtaacggtt gcgctcacgt tcaccaggtc cacgccgccc
     7921 cggaagagcg gacgctggga tccttgccgc gcggcgggac cggcgacgaa cgcaaccagc
     7981 acgagcagga gcgccgggac gaacctccgg ccaacgatgc gggacatctg cagatgacga
     8041 aggcaaaagc gctgccgacg ggcaggcgcc ggattccggg cgttcccgag ccttgcgtcc
     8101 gtggccgtcg tgagccgccg gccgcgcggc aggacgcaac tgtcacgaag cggggacttc
     8161 agccggcgga accctgcggg ccggccggct tccatccctc ggtgaagcgc tgcccctccc
     8221 gctcggcgag gaggcgaacc ggcctgaagc cggcggatgt cagccacgcc gccaccgagg
     8281 cggccggcgg ccagctggcc gcgccgcgtc ccgcgagcgc cgccagcacg ccgcgtggct
     8341 gccggtcgag gacgagcgcg cgaccgcccg gcctcagcgc ccggagcacc tcctccgcga
     8401 ggtgcgctcg aaccgtctcg ccgagagacg cgagcaaccc gtcggtgttg tcgaccacgg
     8461 cgacgtcgaa ggaggcgtcg ccgacgggca ggccgggcca ggcggtaacc tccacgtcgg
     8521 ccaggacgcc ggctctggcc gcggcagcct tgacgcgcac cgcgtccggc tcggaaccga
     8581 cgacggcgca ggcgtggccc gtgaggccga ccttgccggc catctccgcg aacaaggcgc
     8641 cctggcgggc accgacgtcg aggaaccggt taccgaggcg caggcctacg accgcgagcg
     8701 gcagcgcgtg cggcccgggc cccttccgga agatcctcat cggtgagtaa tgatatagtt
     8761 ctcggccgtg aaacaaccag tcgaaatcac ccaacccacg ggaaagctcg gcatcctcct
     8821 ggtaggcctc ggcgccgtca gcaccacctt catcgcgggc gtcatggccg ttcgcaaggg
     8881 gattgcctca ccgatagggt cgctcaccca gatgggaacc attcgcctcg gcaggcgcac
     8941 cgagggccgc tccccgctgg tgaaggacct ggtgccgctg gcctcgctcg aggacctcgt
     9001 cttcggcggc tgggacatct tcgaggacga cagctactcg gccgcgcaca ccgcgggcgt
     9061 tctcgacgag agcctgctcg agcagatccg ccccgagctc gaggcgatca ctccgtggcc
     9121 ggccgtcttc gaccgccggt acgtcaagcg gctcgacggc cccaacgtca agcgcggctc
     9181 gaacaagatg gagctggcgc agcaggtgcg cgaggacatc cgccgtttca aggccgagaa
     9241 cggcctcgcc cggctggtga tgatctggtg cggcagcacc gagatcttca tgaccccgtc
     9301 gcccgctcac cagtcgatcg aggcgttcga ggccgggctc tgcgcgaacg acctcaccat
     9361 cccgtccagc atggtctacg cgtacgccgc gctgaaggag ggaatcccct tcgccaacgg
     9421 cgcgccgaac ctgacggtgg acgtgccggc cctgatggag ctcgcccagc agcagggctg
     9481 cccggtggcc ggcaaggact tcaagaccgg gcagacgctc atcaagaccg tcatcgcgcc
     9541 cggactgaag gcgaggctga tcggtgtgcg cgggtggtac tcgaccaaca tcctcggcaa
     9601 ccgcgacggc gaggtgctcg acgatcccga gtcgttcaag accaaggagg agagcaagaa
     9661 gtcggtcctc gactacatcc tccagcccga cctctacccg cagctgtaca aggatctctg
     9721 ccacgtcgtc cggatcaact actacccgcc gaggggcgac aacaaggaag gctgggacaa
     9781 catcgacctg gtcgggtggc tgggctatcc gatgcagctg aagatcaact tcctctgccg
     9841 cgacagcatc ctggccgctc cgatcgtgct cgacctcgtc ctgttcctcg acctcgcggc
     9901 ccgcgccggc tggagcggga tccaggagtg gctctcgttc tacttcaaga gcccgatgca
     9961 cgcgccgggg ctgtaccccg agcacgacct gttcatccag ctgatgaagc tgaagaacac
    10021 gctgcgctac ctgaagggcg aggagctgat cacgcacctg gggcgcgagt actacgactg
    10081 acgcccgcaa gggagaaccg agaacgcagg tagaagtgaa cggataggga gaccgcccat
    10141 gccgtccagc aggatgcagc ccgcgttgtt cggtggcctg gtgctcgggg tcctgtcggc
    10201 gctgccggtc gtcagcgccg gcaacgtgtg ctgctgcctc tggctcatcg cgggcggggt
    10261 ggtggcggcc tacgtcctcc agtcgaacca ggcgacgccg atcacgacgg gcgacggtgc
    10321 catcgctgga ctcctggccg ggatcgtcgg ctcgttcgtc tatctggttc tctcggtccc
    10381 gatcagcctc ctcaccggcc cgctgcagaa ccgctgggtc gagaaggtcc tcgagtcggg
    10441 cggcgaggtg cccgaggcga tgcgacccgc cctcgaggcg atgcgccagg ggggcctctc
    10501 ggtgctgggc gtcgtcttcg gattcgtgat gatgctggtg ctgagcctgc tgttctccac
    10561 cgccggcggc gccattggcg ccgccatctt ccgcaagaag acaccgcccc cgccgccggc
    10621 cacggagtac ggcgcgccgt cgcctcctcc tcccgcgggg atgtagcgct atcggagagg
    10681 ggaacaccgg cgggggggca accgtattga tctggtagcc ccggcagccg cgcccggccg
    10741 cccgtgacac aggaagcacc ttcgatggca ctgatcgaga ctcacgatct ctggaagagc
    10801 tacacgatgg ggagcgagca gattcacgcc ttgagcggcg tgtcgctcgc gatcgaacgg
    10861 ggcgagtacg tggccatcat ggggccctcg ggctccggga agtccacgct catgaacctg
    10921 atcggctgcc tcgacacgcc cagccgcggc agctacctgc tcaacgggaa gcaggtcagc
    10981 cagatgaatg acgacgaact ggcgcggatc aggaacgagg agatcggctt cgtcttccag
    11041 accttcaacc tgctgcctcg ggccacggcc ctgcacaacg tcgagctgcc gctcgtgtac
    11101 gcgggggtgc cgtctgccga ccggctgaag cgcgcccgcg ccgcactcga gagggtggag
    11161 ctgggcgacc gtgtcagcca ccgtcccaac cagctttcgg gcggccagcg gcagcgcgtg
    11221 gcgattgccc gggcactcgt caacaacccg tccatcctgc tcgccgacga gccgacgggc
    11281 aacctcgact cgaagacggg cgaggagatc atggcgctct tcgagcgcct ccaccacgcg
    11341 ggcaacacca tcgtcctggt cacccacgag gccgacatcg ccgcccacgc gcaccgcgtc
    11401 ctgcacatcc gcgacggcaa gatcgaggac gacgtgaggc gagcggcctg acggccgcga
    11461 cttcgcgact tcgcgactac gtggactgac acctcaacag gcgtttcgtg cattcgtcct
    11521 gcgggtcgcc gaagatcgcg tcggccgtgc cggcctcgac gattcggccg tggtgcatga
    11581 cggccacgtg ggtgcagagg tgccgcacga cgcgcaggtc gtgggcgatg aagagatagg
    11641 tgagcccaag ccggtcgcgc aggtccatca ggaggttgag c
//
LOCUS       DAOPQX010000083        11646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6378_length_11646_cov_4.417738, whole genome shotgun sequence.
ACCESSION   DAOPQX010000083 DAOPQX010000000
VERSION     DAOPQX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_11646_cov_4.417738"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKK95_06455"
     CDS             <1..448
                     /locus_tag="PKK95_06455"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17887.1"
                     /translation="GIEETSYEVNPEPTDCVLCGLCTRACDRMGVSAIASVSRGASRA
                     IAPPFDQPPPDCIGCLACAEVCPTGCIAWSEAGGRRTIWGRVFEMLRCEGCGRSHLTD
                     AQAAWARAHGAAEQDLRLCDACKREQLGRTLRAVSRGETVVAQVAT"
     gene            510..1010
                     /locus_tag="PKK95_06460"
     CDS             510..1010
                     /locus_tag="PKK95_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC17888.1"
                     /translation="MAIVLRVVTDRCNGCGNCEMACAFVHARGTRPAQPRIHVLGRQT
                     SPGCGTPILCLQCDEPACVEVCPIQALSRDEATGAIRLDEARCIRCRSCVAACPFGNV
                     AWDAAQRAVVKCDLCGGDPQCARFCPTGALSSRTGVSPVRDTAASPVRDAGVSPVRDP
                     GVPACS"
     gene            1496..3577
                     /locus_tag="PKK95_06465"
     CDS             1496..3577
                     /locus_tag="PKK95_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC17889.1"
                     /translation="MAHAPIKYVERGLGIVANGLWHGYALASRLLQRESFTPRWSDKP
                     LQKSWEKTKPGLGVPRETDSLCPVCVREARREILDGVKDVSVLLNEKVGEIKAQIIER
                     DGKILMVKDCPQHGHFEDVMSIDPEMFRHLERVFPGRDITAHNDEKLHNHGSSTIKYG
                     RGSVLTIDLTNRCNMMCDPCFMDANQVGFVHELSWEDIKTALDNAISIKPRRQMSVQF
                     SGGEPTISPHFLDAIRYAVKLGYRSVQAATNGIEFAKSPEFCRQAAEAGLRFAYLQFD
                     GIGNAANSHRRVGNLFDVKLRAIENLWNAGVSIVPVVTIVNGVNNEQVGRIVEFALDN
                     PKKITFLSFQPVSFTGRDEEVSPERRAAQRYTLAHLAHDVKRQTGMGEPARDWFPISF
                     MGTFSDWADQVHGPGDEWGQLSCGCHPNCGIGMAVMVDKETKERAAVTSFLNGPRLAS
                     DVRLVNDAALGRAWAVLGMALSLLRNYDPFKAPSRLNVLLLMERFDKAFGATKRDYGR
                     LGEGRTMKDIEKRRSDRFLFLFIAGMWFQDLFNYDFRRTEMCIIPYATQMGEISFCAY
                     NTGIGWRQIVEKMHMTATLTKWYEEHGRHEIFAGNKHVPLPSTEHSLSLDPEAVAAGA
                     QHDLEELGIARNAREEQLMARKRKAPTAPTPEEQRRDQEMARLYRQHVLKEDPNLVSV
                     DTLRASLRKSA"
     gene            complement(3654..4619)
                     /locus_tag="PKK95_06470"
     CDS             complement(3654..4619)
                     /locus_tag="PKK95_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC17890.1"
                     /translation="MRPFLLTCLLAASLGVGLRAQQAPVFRGATETVPVYVTVTDGDS
                     RLVTTLTKDDFVLLDNGRKQPIPVFDDSPRPIRLIVMLDISGSMYGNLGLLRAACSQL
                     FSKLRPEDQAKVGWFGEEIKISPEFTNDRAALVAELPAQAPRSAPTPLWTALDAALGA
                     FEGAGDERRVVLVLSDGKDSGPPKFGGRYLTLVDVMERARRDEVMIYSVGLRSRSGRL
                     PGGFGGGFGGLGNRGGAAALGDDFPDPGLGKLALETGGGYFELRPSDDLPATFERVAD
                     ELHAQYLLGFAPPDRDGKRHKIEVKLVPRGLESRARKNYIAPKGS"
     gene            complement(4653..5558)
                     /locus_tag="PKK95_06475"
     CDS             complement(4653..5558)
                     /locus_tag="PKK95_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC17891.1"
                     /translation="MSTHLSRRSFIVTGAAAAASLAAVRPSDLFAQAGKKPPFTISLA
                     EWSLHRELQKKVLDNLDFAKVTRTVFDIDAIEYVNQFFKDKAEDAAYLAELNKRAKDH
                     NVHQHLIMVDGEGQLGDPDEAARKKAVENHYRWVTAAKTLGCASIRVNAASKGTPEEQ
                     MKLAADGLRRLCEFAGQHQINVIVENHGGLSSHGDWLVGVMKLVNHPRCGTLPDFGNF
                     YEYDRYKGVAELMEYAKGVSAKTHDFDAQGNETTKDYPRLIKIMLDAGYHSWVGIEYE
                     GEKLPEREGIAASLRLLKKMQAQFA"
     gene            complement(5568..7829)
                     /locus_tag="PKK95_06480"
     CDS             complement(5568..7829)
                     /locus_tag="PKK95_06480"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOC17892.1"
                     /translation="MSTIKRLGLLTSGGDASGMNAAVRAVVRTALDRGVEVFGISEGY
                     AGLIEGGSRIQPLSWSSVGGILHRGGTTIGTARSDEFRTRPGRLTAARNVLAHGLDAL
                     VVIGGDGSLHGANLLRSEWPGLVEELVAAGQVSPADAALHPVLVVVGLFGSIDNDAAG
                     TDMTIGADTALHRITEAIDAISSTASSHRRTFVVEVMGRHCGYLALMSAIATGSDWVF
                     IPENPPKSDRWEDEMLASLKAARDAGRRDATVVVAEGATDREGRPITSERLRDVLGKG
                     LGESVRITVLGHVQRGGAPSAYDRNLGTIMGHAAVETLLGGHADRESQVIGMRGNRVE
                     RIPLAESVAQSREVNRLLESREYARALEMRGPSFNTALGTMQTLLRALPHPPQAGQRR
                     FRLAIMHAGAPAPGMNAAVRAAVRVALDRGHIVLGVQGGFPGLVDDDIKTMDWMSVDG
                     WASRGGAELGTNRAVPDGPDFYAIARTIEKRRIEGLLIIGGWEAYQGAHRLFEERRNY
                     PAFEIPLVCLPATIDNNLPGTELSIGADTALNSIVTVVDKIKQSAVAERRCYVVEVMG
                     RRCGYLASMSGLATGAERVYLPEEGITLKAMKEDLDALIRGFQEGKRVGLVIRNEEAN
                     PTYDTAFISALFDEESGGVFAVRQSILGHLQQGGDPSPFDRIQGTTLARQCVSFLIER
                     AAEANPVAAFIGTVSGRLEFHPLEDLPRMVDEPNRRPKHQWWLSLRGINDALARPAPG
                     APRKSRERGVEMR"
     gene            complement(7896..10901)
                     /locus_tag="PKK95_06485"
     CDS             complement(7896..10901)
                     /locus_tag="PKK95_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOC17893.1"
                     /translation="MSKRIVFIACLLAMAAPAVAGTSYYTVRLDDPKAVHLEAFGARG
                     DGVADDSDAIQRAIDRVHETVGPGIVFVPEGRYRITRTIDVWPSIRLIGYGATRPVIV
                     LGPNTPGYQDRNAERQMIFFAGSRPGFGRPGAPPAAAPVPGARPPDASPGTFYSAISN
                     IDIEIGEANHGAVAVRGTYAQHCFLAHMDIRVGPGIAGVHDTGNVMEDVRFFGGTYGI
                     WTRTPSPGWQFTAVDTYFEGQREAAIRERAAGLTLVRPHFRNVPAAVSIDEDSHDELW
                     IKDGRMEEISGPAILIGLEENPRTQINFENVVCRRVPSFARLRKSGKTFAAPGEIYEV
                     KVFSHGLHYAGIRAAPAIVDVFEANALEAMPEPVASDLVPLPPAGSWVNVHALGAKGD
                     GVTDDTEAFRRAIASHRAIFLPMGYYVISDTLTLRPETVIVGLHPLVTALVLLDRTEA
                     FQGVGPPKAMIETPKGGTNVMIGVGVYTNGINPRAVGVKWMAGARSMMNDVRFLGGHG
                     TNNLDGTRANPYNNSRTADPDANRRWDSQYPSLWVTDGGGGTFFDIWTPSTFAMSGML
                     ISDTTTEGRVYQMSSEHHVRYEIQVRNAANWRIYAMQTEEERGESPFALPLEVVDSEN
                     ITFANFHLYRVISSYQPFPWAVKVSNSRNIRFRNFHCYSNSKVSFDAAVVDEDSGAEI
                     RQREFAWLDVPGRAPAGRTQVGRGSAGQGSVESSVARSNILEPGAEVRRLATGFFNIS
                     GGAAVPSGDFFFVDARRQRIYRWDVSRSRLSVERDTPLEPVNLAADRAGNLMVVSYGG
                     NGTVYAFEPGSPIDQLSVLEPVDATLRPELTAVRPVGDWRVARDPETGQAVRRPFHYL
                     SPDGTTYISAGRDFTTGAQSWGVKSADLLRGFGLAAARPGQPFYVTSESEVTTWRGTL
                     APDGNLEGLEVFVQQGGEGVAVDAEGRVYLAAGHIQVYDPNGRPIETIEVPERPTQLV
                     FGGADGKTLFICARTSLYAVRTQVPGRQP"
     gene            10993..>11646
                     /locus_tag="PKK95_06490"
     CDS             10993..>11646
                     /locus_tag="PKK95_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOC17894.1"
                     /translation="MRHTGLLGAAAVSFVLGVSAGVSAQATVKTEGGEVRGAAIGTTG
                     IRVFKGVPYAAPPTGERRWTAPQPPPAWEGVRDATAFGPGCVQGKVFGDIHFDRQDED
                     CLNLNIWTPAKGGERLPVMVWIHGGGFVAGGGGEPRHDGEAFARKGIVLVSINYRLGV
                     FGFFAHPELTRESGRSASGNYGLLDQVAALRWVRDNIAAFGGDPANVTIFGESAGSFS
                     "
BASE COUNT         1696 a         4121 c         4037 g         1792 t
ORIGIN      
        1 cggcatcgag gagacctcct acgaggtgaa ccccgagccg accgactgcg tgctgtgcgg
       61 cctctgcacg cgggcctgcg accggatggg ggtgtcggcc atcgccagcg tctcgagagg
      121 agcctcgcgg gcgatcgcgc cccccttcga tcagccgccg ccagactgca tcgggtgtct
      181 cgcttgcgcc gaggtctgcc cgacgggctg catcgcctgg agcgaggccg gcggccggcg
      241 caccatctgg ggccgcgtct tcgagatgct gcgctgcgag gggtgcgggc gctcccacct
      301 caccgacgcg caggcggcgt gggcccgcgc gcacggtgcg gccgaacagg acctgcggct
      361 gtgcgacgcc tgcaagcgcg agcagctggg gcggacgctc cgcgcggtgt cgcgaggcga
      421 gacggtggtc gcgcaggtgg caacctgacg ccctggcgct gctcgggaca cggaaggcgg
      481 gaagcagcct tctgccagtt ggagacgaga tggctatcgt gctgcgcgtg gtcacggatc
      541 gctgcaacgg gtgcggcaac tgcgagatgg cctgcgcctt cgtccacgcg cggggcaccc
      601 gtcccgcgca gccgcgcatc cacgtgctcg gccggcagac ctcgccgggc tgcggcacac
      661 caatcctgtg cctgcagtgc gacgagcccg cctgcgtcga ggtgtgcccg atccaggcgc
      721 tgtctcgcga cgaagcgacg ggcgccatcc ggctcgacga agcccgctgc atccggtgcc
      781 ggtcgtgcgt ggccgcctgc ccgttcggca acgtggcgtg ggacgcggcc cagcgcgcgg
      841 tcgtgaaatg cgacctgtgc ggcggcgacc cccagtgcgc ccgcttctgc ccgaccggcg
      901 ccctctccag tagaacaggc gtctcgcctg ttcgtgatac cgccgcctcg cctgttcgtg
      961 atgccggcgt ctcgcctgtt cgtgatcctg gcgtccccgc ctgctcgtga tcggcgtccc
     1021 cgcacgctcc tgacctcgcg acgccctgca ccttcgaagg gcgccacgga tcaaccacac
     1081 ttcgtccagc cacccggcgt cggcgggagc tgtctcaagg cttgccggtc ttcgggcggt
     1141 cggccacctg gtcgatcgca gcggccgccg aagaccggct cggtgtcctg cgtgagagcg
     1201 tgaagcccgt ccatgagcgg ttctgcggcc ggggcagcgc ccagaaaccc tcaccgacga
     1261 ggtggaacgc cgcgttccgt cgcgcccgct cgccccccga ttgcgccccc tcgccggaga
     1321 atcgctgatc ccccgcgtgg gcccgcctac cctgaggtca aaggaaacgg ccggcatccg
     1381 cctcggctgc agcgcggtcg cgcacgggtc ttcgtggtcg cggctgcggc gcgggcggag
     1441 gccggtgcgg acacgcgggc gatcaccacg gtcgccccta cgggaggtgc cccccatggc
     1501 tcacgccccc atcaagtacg tcgagcgagg cctcggcatc gtcgccaacg gcctgtggca
     1561 cggatacgcg ctggcgagcc ggctcctgca gcgggagtcg ttcacgcccc gctggtcgga
     1621 caagccgctg cagaagtcgt gggagaagac gaagcccggc ctcggcgtac cgcgcgagac
     1681 cgattcgctc tgcccggtct gcgtgcgcga ggcgcgccgc gagatcctcg acggcgtcaa
     1741 ggacgtctcg gtgctgctga acgagaaggt cggtgaaatc aaggcgcaga tcatcgaacg
     1801 cgacgggaag atcctgatgg tcaaggattg cccgcagcac gggcacttcg aagacgtcat
     1861 gtcgatcgac ccggagatgt tccggcacct cgagcgggtg ttccccgggc gcgacatcac
     1921 cgcgcacaac gacgagaagc tgcacaacca cggcagcagc accatcaagt acggccgcgg
     1981 gtcggtgctg acgatcgacc tcaccaaccg ctgcaacatg atgtgcgatc cctgcttcat
     2041 ggacgcgaac caggtggggt tcgtccacga gctgtcgtgg gaggacatca agaccgcgct
     2101 cgacaacgcg atctccatca agccccgccg ccagatgtcg gtgcagttct cgggcggcga
     2161 gccgaccatc tcgccgcact tcctcgacgc gatccgctac gcggtgaagc tcggctaccg
     2221 cagcgtgcag gcggcgacca acggcatcga gttcgccaag agccccgagt tctgccgcca
     2281 ggcggccgag gccggcctcc gcttcgccta cctgcagttc gacgggatcg gcaacgcggc
     2341 caactcgcac cggcgcgtcg gcaacctctt cgacgtcaag ctccgcgcca tcgagaacct
     2401 ctggaacgcc ggcgtcagca tcgtccccgt cgtgacgatc gtcaacgggg tgaacaacga
     2461 gcaggtcggg cgcatcgtcg agttcgccct cgacaacccg aagaagatca ccttcctgtc
     2521 gttccagccg gtgtccttca ccggccgcga cgaggaggtc agccccgagc ggcgggccgc
     2581 gcagcgctac acgctggcgc acctggcgca cgacgtgaag cgccagacgg ggatggggga
     2641 gccggcgcgc gactggttcc cgatctcctt catgggcacc ttttccgact gggccgacca
     2701 ggtgcacggg ccgggcgacg agtggggcca gctgagctgc ggctgccacc cgaactgcgg
     2761 catcgggatg gccgtcatgg tggacaagga gacgaaggag cgggcggcgg tgacctcctt
     2821 cctgaacggc ccccggctgg cgagcgacgt gcggctggtg aacgacgccg cgctgggccg
     2881 ggcctgggcg gtgctcggga tggcgctgtc gctgctccgc aactacgacc cgttcaaggc
     2941 gccctcgcgc ttgaacgtcc tgctgctgat ggagcggttc gacaaggcct tcggcgccac
     3001 caagcgcgac tacggccgcc tcggcgaggg ccggacgatg aaggacatcg agaagcggcg
     3061 atcggaccgc ttcctgttcc tgttcatcgc cgggatgtgg ttccaggacc tgttcaacta
     3121 cgacttcagg cggacggaga tgtgcatcat cccgtacgcc acccagatgg gcgagatctc
     3181 cttctgcgcc tacaacaccg ggatcggctg gcggcagatc gtcgagaaga tgcacatgac
     3241 ggccaccctc acgaagtggt acgaggagca cggccgccac gagatcttcg ccgggaacaa
     3301 gcacgtcccg ctcccctcga ccgagcactc gctctcgctc gatcccgagg cggtggcggc
     3361 tggcgcccag cacgacctcg aggagcttgg catcgcgcgc aacgcgcgcg aggagcagct
     3421 gatggcgcgg aagcgcaagg cgccgacggc gccgacgccg gaggaacagc ggcgcgacca
     3481 ggaaatggcg cggctgtacc gccagcacgt gctcaaggag gacccgaacc tggtgtcggt
     3541 ggatacgctg cgggcgtcgc tgcggaaatc ggcgtaggga cagcattggg gcgaccacaa
     3601 gggtcgcccc tacgttgtcg gctggcgtca gctgcccgcg ctgtccgctg tcatcagctg
     3661 cctttcggcg cgatgtagtt cttccgcgcg cgcgactcga ggccgcgcgg gacgagcttc
     3721 acctcgatct tgtggcgctt gccgtcgcga tccggcggcg cgaagccaag caggtactgc
     3781 gcgtgcagct cgtcggccac gcgctcgaag gtggccggga ggtcgtcgga gggccgcagc
     3841 tcgaagtagc cgccgccggt ttcgagcgcc agcttgccga ggccggggtc gggaaagtcg
     3901 tcgccgagcg cggcggcgcc gccacggttg cccagaccgc cgaagccgcc gccgaatccg
     3961 cccggcagcc ggcccgagcg ggagcgcagc cccaccgagt agatcatcac ctcgtcgcgc
     4021 cgggcgcgct ccatcacatc caccagcgtg aggtagcggc cgccgaactt cggcggcccc
     4081 gagtccttgc cgtcgctcag cacgaggacg acgcgccgct cgtccccggc gccctcgaac
     4141 gcgccgagcg cggcgtcgag cgcggtccag aggggggtcg gcgcgctgcg cggggcctgc
     4201 gccggcagct cggccaccag cgccgcccgg tcgttggtga actcggggct gatcttgatc
     4261 tcctcgccga accaccccac cttcgcctgg tcctcgggcc ggagcttcga gaacagctgc
     4321 gagcaggcgg cgcgcagcag ccccaggttc ccgtacatgc tcccgctgat gtcgagcatg
     4381 acaatgagcc ggatgggccg cggcgagtcg tcgaagaccg ggattggctg cttgcgtccg
     4441 ttgtcgagca ggacgaagtc gtccttggtg agcgtggtga cgagacggct gtccccgtcc
     4501 gtcacggtca cgtagaccgg caccgtctcg gtggccccgc ggaagaccgg cgcctgctgg
     4561 gcgcggagcc ccacgcccag cgaggccgcc aggaggcagg tcagcagaaa gggtcgcatg
     4621 accccaacat accgccggcg gggaggccgg cgctaggcga actgggcctg catcttcttg
     4681 agcaggcgca gcgacgccgc gatcccctcg cgctcgggca gcttctcgcc ctcgtactcg
     4741 atcccgaccc acgagtggta ccccgcgtcg agcatgatct tgatcaggcg cgggtagtcc
     4801 ttcgtggtct cgttgccctg cgcgtcgaaa tcgtgcgtct tggcgctgac ccccttcgcg
     4861 tactccatca gctcggcgac gcccttgtag cggtcgtact cgtagaagtt cccgaagtcg
     4921 ggcagcgtcc cgcagcgcgg gtggttcacc agcttcatca cccccaccag ccagtcgccg
     4981 tgcgacgaca ggccgccgtg gttctcgacg atgacgttga tctggtgctg cccggcgaac
     5041 tcgcacagcc ggcgcagccc gtcggccgcc agcttcatct gctcctcggg cgtccccttg
     5101 gaggcggcgt tcacccggat gctcgcgcac cccagcgtct tcgcggcggt cacccagcgg
     5161 tagtggttct cgaccgcctt cttccgcgcg gcctcgtccg ggtcgcccag ctgcccctcg
     5221 ccgtccacca tgatgaggtg ctggtggacg ttgtggtcct tggcgcgctt gttcagctcg
     5281 gccaggtagg ccgcgtcctc ggccttgtcc ttgaagaact ggttcacgta ctcgatggcg
     5341 tcgatgtcga agacggtgcg cgtcaccttc gcgaaatcga ggttgtcgag caccttcttc
     5401 tgcaactcgc ggtgcagcga ccactcggcc agcgagatcg tgaacggcgg cttcttgccc
     5461 gcctgcgcga agaggtcaga cgggcgtacg gctgccagcg atgccgcggc tgcggccccg
     5521 gtgacgatga aggaacggcg cgacaggtgt gtgctcatgt ttgaaggcta cctcatctcg
     5581 acaccgcgtt cacgcgactt gcggggcgcg cccggtgcgg gccgcgccag cgcgtcgttg
     5641 atcccgcgca agctcagcca ccactggtgc ttcggccggc ggttcggctc gtccaccatc
     5701 cgcggcaggt cctcgagcgg gtggaactcg agcctgcccg acacggtgcc gatgaacgcc
     5761 gccaccggat tggcctcggc cgcacgctcg atcaggaagc tcacgcactg ccgggcgagc
     5821 gtcgtcccct ggatgcggtc gaagggcgag gggtcgcccc cctgctgcag gtgcccgaga
     5881 atgctctgcc tcaccgcgaa cacgccgccg ctctcctcgt cgaagagcgc cgagatgaag
     5941 gccgtgtcgt aggtggggtt cgcctcctcg ttgcggatca cgaggccgac gcgcttgccc
     6001 tcctggaagc cgcggatgag ggcgtccagg tcttccttca tcgccttgag cgtgattccc
     6061 tcctcgggca ggtagacgcg ctcggcgccg gtcgccaggc cgctcatcga agccaggtac
     6121 ccgcaccggc gccccatcac ctcgacgacg tagcaccgcc gctcggcgac ggccgactgc
     6181 ttgatcttgt ccaccaccgt cacgatgctg ttgagcgccg tgtcggcccc gatgctcagc
     6241 tcggtgcccg gcaggttgtt gtcgatcgtc gccggcaggc acaccagcgg gatctcgaac
     6301 gccgggtagt tgcggcgctc ctcgaacaga cggtgcgccc cctggtacgc ctcccagccc
     6361 ccgatgatca gcagcccctc gatccgccgc ttctcgatgg tccgggcaat cgcgtagaag
     6421 tcggggccgt cgggaacggc cctgttggtg cccagttccg cgccgccgcg cgacgcccac
     6481 ccatcgacgc tcatccagtc catcgtcttg atgtcgtcgt ccaccagccc gggaaagccg
     6541 ccctggacgc cgagcacgat gtggccgcgg tcgagcgcga cgcgcacggc cgccctgacg
     6601 gcggcgttca tgccgggggc cggcgcgccg gcgtgcatga tggccaggcg gaagcgcctc
     6661 tggccagcct gcggcgggtg cggcagcgcg cgcagcagcg tctgcatggt gccgagggcg
     6721 gtgttgaagc tcgggccgcg catctcgagc gcgcgggcgt actcgcgcga ctcgagcagc
     6781 cggttcacct cgcgcgactg ggccaccgac tcggcgagcg gtatccgctc gacccggttg
     6841 ccgcgcatcc cgatgacctg cgactccctg tcggcgtgcc cgccgagcag tgtctcgacc
     6901 gccgcgtgcc ccatgatcgt gccgaggttc cggtcgtacg cgctcggcgc cccgccgcgc
     6961 tgcacgtgac cgagcacggt gatccggacc gattccccga gccccttccc gagcacgtcg
     7021 cgcagccgct cgctcgtgat cggccgcccc tcgcggtcgg tcgccccctc ggccaccacc
     7081 accgtcgcgt cgcgccggcc ggcgtcgcgg gccgccttca gcgacgccag catctcgtct
     7141 tcccacctgt cggacttcgg cgggttctcg gggatgaaca cccagtccga gccggtggcg
     7201 atggcgctca tcagcgccag gtagccgcag tgccgcccca tcacctccac cacgaacgtc
     7261 cggcggtggc tcgaggcggt gctgctgatg gcgtcgatcg cttcggtgat ccggtggagg
     7321 gcggtgtcgg cgccgatcgt catgtcggtg ccggccgcgt cgttgtcgat cgatccgaac
     7381 aggcccacga cgaccaggac gggatgaagg gcggcgtccg cggggctgac ctggccggcg
     7441 gcgaccagct cctccaccag gcctggccac tcggagcgca gcaggttggc gccgtggagg
     7501 ctgccgtcgc cgccgatgac gacgagggcg tcgaggccgt gggccaggac gttgcgcgcc
     7561 gcggtgagcc gccccggccg ggtgcggaac tcgtcggagc gcgcggtgcc gatggtggtg
     7621 ccgccgcggt ggaggatccc gccgaccgag ctccagctga gtggctggat ccggctgccg
     7681 ccttcgatga gaccggcgta tccctccgag atgccgaaca cctcgacgcc ccggtcgagc
     7741 gccgtgcgca cgacggcgcg caccgcggca ttcatcccgg acgcatcacc cccgcttgtc
     7801 aacaggccga gacgcttgat ggtactcacg gaggcaatgt tacgacagaa gacggcctcc
     7861 cgggcttccg cctccgcgcc gccccccgcc tccagctacg gctggcggcc gggcacctgt
     7921 gtacgcacgg cgtagagcga cgtccgcgcg cagatgaaca gcgtcttccc atcggcgccg
     7981 ccgaagacga gctgcgtcgg acgctcgggc acctcgatcg tctcgatcgg ccgcccgttc
     8041 gggtcgtaga cctggatgtg cccggccgcg aggtacacgc gcccctcggc gtccaccgcc
     8101 accccctcgc ccccctgctg cacgaagacc tcgaggcctt cgaggttgcc gtcgggtgcg
     8161 agtgtgccgc gccaggtggt cacctccgac tcgctggtca cgtagaacgg ctggcccggc
     8221 ctggcggcgg ccagtccaaa gccgcgcagc aggtcggcac tcttcacccc ccagctctgg
     8281 gcgccggtgg tgaagtcgcg gccggccgag atgtaggtgg tgccgtctgg cgacaggtag
     8341 tgaaacggcc gccggacggc ctgccccgtt tccgggtcgc gcgccacgcg ccagtcgccg
     8401 acgggccgca ccgccgtcag ctctggccgc agggtggcgt caaccggctc gagcaccgac
     8461 agttggtcga tcggcgagcc cggctcgaat gcgtaaacgg ttccgttccc gccgtaggag
     8521 acgaccatca ggttgccggc gcggtcggcg gccaggttga ccggctcgag gggcgtgtcg
     8581 cgctcgacag acagccgcga ccgcgagacg tcccaccggt agatgcgctg gcgccgcgcg
     8641 tccacgaaga agaagtcgcc ggagggaacc gccgcgccgc cggaaatgtt gaagaagccg
     8701 gtggcgaggc gccgcacctc cgcgcccggc tcgaggatgt tcgatcgggc aacgctgctt
     8761 tcgacactgc cctggccggc cgagccgcgc cccacctgtg tacgtcccgc aggggcacgc
     8821 cccggcacgt cgagccaggc gaactcgcgc tgcctgatct cggcgccgga gtcctcgtcg
     8881 acgaccgccg cgtcgaacga caccttgctg ttgctgtagc agtggaagtt ccggaagcgg
     8941 atgttccgcg agttcgaaac cttcaccgcc caggggaacg gctggtacga gctgatgacc
     9001 cggtagaggt ggaaattggc gaaggtgatg ttctccgagt cgacgacctc gaggggcagc
     9061 gcgaaggggc tctcgccgcg ctcctcctcg gtctgcatcg cgtagatccg ccagttggcc
     9121 gcattccgca cctggatctc gtagcggacg tggtgctcgc tcgacatctg gtagacgcgc
     9181 ccctcggtgg tcgtgtcgga gatcagcatc cccgacatcg cgaacgtgct cggcgtccag
     9241 atgtcgaaga aggtgccgcc gccgccgtcg gtcacccaca ggctcgggta ctggctgtcc
     9301 cagcggcggt tggcgtcggg atcggccgtc ctgctgttgt tgtaggggtt tgcgcgcgtc
     9361 ccgtccaggt tgttggtgcc gtggccaccg aggaaccgga cgtcgttcat catcgagcgg
     9421 gcgccggcca tccacttgac gccgaccgcg cgcgggttga tcccgttggt gtagaccccg
     9481 acgccgatca ttacgttcgt cccgcccttc ggcgtctcga tcatcgcctt cggcggcccg
     9541 acgccctgga aggcctcggt ccggtcgagc agcaccagcg cggtgaccag cgggtgcagg
     9601 ccgacgatca ccgtctcggg ccggagcgtc aacgtgtcgg agatgacgta gtaccccatg
     9661 gggaggaaga tggcgcggtg cgacgcgatc gcccgccgga aggcctcggt gtcgtcggtg
     9721 acgccgtcgc cctttgcgcc gagcgcgtgc acgttcaccc agctcccggc cggcggaagc
     9781 gggacgaggt ccgacgccac cggttcgggc atcgcctcca gcgcgttggc ctcgaagaca
     9841 tccacgatcg cgggcgccgc gcgtatgccg gcgtagtgaa ggccatgcga aaagaccttc
     9901 acctcgtaga tctcgcctgg cgcggcgaac gtcttcccgc tcttccgcag acgggcgaag
     9961 gaggggacgc ggcggcagac cacgttctcg aagttgatct gtgtccgggg gttctcctcg
    10021 aggccgatga ggatggccgg gcctgagatc tcctccatcc ggccgtcctt gatccacagc
    10081 tcgtcgtgcg agtcctcgtc gatcgagacg gcggccggga cgttccggaa gtgcgggcgg
    10141 accagcgtca ggccggcagc gcgttcgcgg atggccgcct cgcgctgccc ctcgaagtac
    10201 gtgtctaccg ccgtgaactg ccagccgggg gacggcgtcc tggtccagat gccgtaggtg
    10261 ccgccgaaaa agcggacgtc ctccatcacg ttgccggtgt cgtgcacccc cgcgatgccc
    10321 ggcccgacgc ggatgtccat gtgcgcgagg aagcagtgct gggcgtaggt gccgcgcacc
    10381 gcgacggcgc cgtggttggc ctcgccgatc tcgatatcga tgttgctgat ggccgagtag
    10441 aaggtgcccg gcgaggcgtc gggcggacgc gcgccgggga ccggggccgc ggccggcgga
    10501 gcgcccgggc ggccgaaccc cgggcgcgac ccggcgaaga agatcatctg acgctcggcg
    10561 ttccggtcct ggtagccggg ggtgttcggg ccgagcacga tcaccgggcg cgtggcgccg
    10621 tacccgatca gccggatgga cggccagacg tcgatcgtgc gcgtgatccg gtaccgcccc
    10681 tcgggcacga agacgatgcc cggccccacc gtctcgtgca cccggtcaat ggcgcgctgg
    10741 atggcgtcgg agtcgtcggc gacaccgtcg ccgcgggcgc caaacgcctc gaggtgcacg
    10801 gccttcggat cgtcgaggcg gacggtgtag tacgacgtcc cggccaccgc cggcgccgcc
    10861 atggcaagca gacaggctat gaacacgatg cgcttggaca tggtcatccg ccttcgccct
    10921 cacatcaaag tcccgccttg acgcgccact atagctccga catgatcgct ccgccagagg
    10981 gaggggcatc gcgtgcggca caccggatta ctcggagcgg cggcggtttc gttcgttctt
    11041 ggcgtgtcgg ccggcgtctc ggcgcaggcg accgtgaaaa ccgaaggcgg cgaggtccgc
    11101 ggtgccgcga tcggcaccac cggcatccgc gtcttcaagg gcgttcccta cgccgccccg
    11161 cccaccggcg agcggcggtg gacggcaccg cagccgcccc cggcgtggga gggcgtccgc
    11221 gacgcgaccg cgttcgggcc cggctgcgtg caggggaagg ttttcggcga catccacttc
    11281 gaccggcagg acgaggactg cctcaacctc aacatctgga cgcccgcaaa gggcggcgag
    11341 cgcctcccgg tgatggtctg gatccacggc ggcggcttcg tcgccggcgg cggcggcgag
    11401 ccgcgtcacg acggcgaggc cttcgcccgc aagggcattg tcctggtttc catcaactac
    11461 cgcctgggcg tcttcggctt cttcgctcac cccgaactga cccgcgagtc ggggcgcagc
    11521 gcctcgggca actatggcct gctcgaccag gtggccgccc tccggtgggt gcgcgacaac
    11581 atcgcggcgt ttggcggcga cccggcgaac gtcacgatct tcggcgagtc ggccggatcg
    11641 ttctcg
//
LOCUS       DAOPQX010000084        11620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6400_length_11620_cov_4.695460, whole genome shotgun sequence.
ACCESSION   DAOPQX010000084 DAOPQX010000000
VERSION     DAOPQX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6400_length_11620_cov_4.695460"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PKK95_06495"
     CDS             complement(<1..802)
                     /locus_tag="PKK95_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17895.1"
                     /translation="MLRRAMLILVVLAAGIAGAFALASYSSAPEPMTENQAPAAERAN
                     PLLTPSPLPYGAPPFDRIQDADFKPAFEAGIRQQLDEIAAIADSAEPPTFENTLVALE
                     RSGQLLTRVNLAFNGLSSANTNPTLQALQEEIAPKLAALDDAIFLNGKLFERVEKLWG
                     QRDALKLAGQDLRLAEYYYRRFVRAGAKLSDEDKTKLKALNEEDAALAAKFTNMLMAA
                     TKAGALVTSNPADLAGLTKDEIQAAAETAKARGLEGQWVVPLQNTTQHP"
     gene            1498..2160
                     /locus_tag="PKK95_06500"
     CDS             1498..2160
                     /locus_tag="PKK95_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOC17896.1"
                     /translation="MQILVATALALLFCAQGGALTQANPAQSKQPAPGARAAEAVAPP
                     AHPVDPTVAADAPDRGFTIGPEDVLGILVWREPEVSGDVTVRPDGMITLPLIRDVKAA
                     GLAPDALADRIQAALREYITDASVTVVVRQMNSRKVFITGEVVRPGSYPLASRMTVMQ
                     LIAVAGGLTEWANARKISLIRVEGGKTTTHPFDYQDVAQGRKPQQNLVLRAGDTVVVP
                     EA"
     gene            2429..3700
                     /locus_tag="PKK95_06505"
     CDS             2429..3700
                     /locus_tag="PKK95_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17897.1"
                     /translation="MRSQEERTSRDPGLTRRELTLAANLNGGYDDNLTGGLGGGAGTA
                     PRIMASGSTGYFDAALGYFYGNALRSVRVDAAGSLQAYPDYLDGVAPGGAATISAQTN
                     LGRRTLFRASERVGYEPLFSVFSPGASSAPLPGGIAETAPAAGLYERRSASSNALLAL
                     DRQWGRRDATSFSYMYRVQRFTSEDYGDNSAQDLGAEHRRTLARGVGLRAAYRHVRRD
                     YTDFAGEGRATREHRIEGGPEIEYTRSRRRSLSLLLAGGAAHVESLSSADRPSYSSWV
                     PIGSGTLTLGLPPNWSLQGGYQRDFALLRGVTDEVYTTDTAFGSAGGLLSSRVDLRLG
                     GSFSRWRTSLVPGLDGRLRTYGGSLQLGIALTGAVAATAGYHYYHHRYSDPAALPEGF
                     PARYNRHAVRVGIAVWVPLMGAISRPTRILR"
     gene            3718..5352
                     /locus_tag="PKK95_06510"
     CDS             3718..5352
                     /locus_tag="PKK95_06510"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOC17898.1"
                     /translation="MIPGQDLTIKFAIRALKRRPWLVAGPLFFGVMGGLLYSRSQPSL
                     YRSDAVVQVVPQRIPDTYVQSTVTARVEDRLSSIAQQVLSRTQLESVILEFDLYPDER
                     RRFPMEDVFELMTERVNVAPLEGSTRGRQQAVDAFRISFDYERPEVARDVAARLAGFF
                     IDQNVKERGSQADQTSAFLESQLADARSRLEAQEKKLETFRERNSGRLPTQTQTNMQA
                     IQNTQMALQAMVESLARDRDRKLMLERLYTDAAADSPVVAPATGPVPSPAPAATDQAS
                     VLPGAPPRQRLEAARAALAQMEVRLSQKHPDVIRTKRLIDDLERQVATEEAQRPLSPD
                     AASERPASQEELRRRDRLREMRAEIESLDRQIAFKESEESRLRNEIGAYQARLEAVPG
                     IESEWIALTRDYDTLQDTYKQLLAKSENSKMAASLEQRQIGEQFRILDPPRVPVKPYS
                     PNRMKINLLGTLAGLGLGLLLIGLAEYRDTTMRTESDLMGAMDLPVLGLLPYVQTEAD
                     GRRLRIRRRFLAAGVLGVAIATGTAFWVLELWKYVV"
     gene            5375..6304
                     /locus_tag="PKK95_06515"
     CDS             5375..6304
                     /locus_tag="PKK95_06515"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOC17899.1"
                     /translation="MGRIDEAYRRLNLDVRRGTGAPASDGDGSPWQPDSLGGGDGRQA
                     AMPAQPSQTAPAPALRWKQQGHEGSERRDRWARLDVEATRRLVVSDQAPPLLIEQYRS
                     LAATLLAAPGERPPRSLIVTSASPGDGKSHVAVNLALTLARSYGRQVLLIDADLRRPV
                     LNRMVGAPNSRGLAEALEAEADERLATVPIEEGLTLLPAGRPTPNHIAGLASERMKRI
                     VDEATARFDWVIVDTPPVGILADGRLVADMVDGVLLVVRAGETPFPDLEAAVDVIGRE
                     RILGMVLNAVDPAEIRGREYYHRYYHGHEARKG"
     gene            6397..6798
                     /locus_tag="PKK95_06520"
     CDS             6397..6798
                     /locus_tag="PKK95_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17900.1"
                     /translation="MEYDEGTFRYFLSVERARAERSNHALRVVFASIELVPGEPARIS
                     RARAAELFEGLRRSLRDTDITGWYRQDRVAAALLSERAEPPGANTLGVIERRVDEGVR
                     QRLPRRIARALKVRVVQLRPRRARNEEELPV"
     gene            7116..7955
                     /locus_tag="PKK95_06525"
     CDS             7116..7955
                     /locus_tag="PKK95_06525"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOC17901.1"
                     /translation="MLGLLLPDASAKSALQTEHRLRREMSRRLDEPQLSALSVHPYSL
                     GDRCGTDPLPPIELVVETFASSRDLSWRDIAKRALDVAGSLAFLVLFSPVFAVVWAAV
                     RWTFDGPAIYRQVRIGRGGEAFTMLKFRTMYVNAEDGIHRDYVSWFIKSSTRQPHAAH
                     EVFKLTDDPRITPLGRFLRRTSLDELPQFWNVLRGEMSLVGPRPPLPFEVEQYKPWHR
                     RRVLEAKPGVTGPWQVRGRSQTTFDEMVRIDLHYARTHSLWTDIKILAATPRAMLSGK
                     GAC"
     gene            7955..8575
                     /locus_tag="PKK95_06530"
     CDS             7955..8575
                     /locus_tag="PKK95_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17902.1"
                     /translation="MDTYLAIAPDVKLGKDVKLARFINLYGCEIGDETKIGAFVEIQK
                     NAKVGRRCKISSHTFICEGVTIEDNVFIGHGVTFINDTYPRATTEGLVGAGERVASPA
                     RGEQHRPGPHLGLGVPRQEIGRPHRLGQRARTVPLAHEGLGQLEPRRCQHRVCFHRPA
                     VLQDGGVHVPLRELAVAPLHEVGRASRAAGHRRRDEDHPHERSSSQ"
     gene            8748..9323
                     /locus_tag="PKK95_06535"
     CDS             8748..9323
                     /locus_tag="PKK95_06535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPDSG-CTERM sorting domain-containing protein"
                     /protein_id="HOC17903.1"
                     /translation="MAGDFQILVTNGDGLTGLPDSFYAFCVDLQHWADTSAGATLGSM
                     AGWDLLDGVTDYQRLGASYLANQYFSLYPDGSASNLTAAYYQVAIWELLHERNPLKVT
                     PFNVWSVTGGSTYFSSGTLTAGAQDLIRGIDTSSPLFTYAGHLITPDHDGYGQNFIAP
                     VPTPVPDGGTTLMLLGGALIGLDLLRRRLRP"
     gene            9644..10366
                     /locus_tag="PKK95_06540"
     CDS             9644..10366
                     /locus_tag="PKK95_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HOC17904.1"
                     /translation="MKRRHLLGLIVAASTLMPPAAGAEPITFFGFDSGLGELARLSSS
                     PNADAARAAFMANLVGAGTEDFESYAAGTTAPLAIAFPGAGTATFFGDGSIASQPSGT
                     NGFGRYPVSGSQFWDRQEAFSIAFSEPIAAFGFYGVDIGDFGGRMRLWLADGGSTYLD
                     VPHDIDQAGGSVMYFGFYDLDQQFVGITFGDNYASGTDFFGLDDLTVASRAQVHPVPE
                     PASALLVGLGLAGLAAVRRRRE"
     gene            10505..11302
                     /locus_tag="PKK95_06545"
     CDS             10505..11302
                     /locus_tag="PKK95_06545"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOC17905.1"
                     /translation="MTGGATLDIPSLPNLRDLGGHRTADGRIVRTGLVYRSDQPSPMT
                     AEDVERLATLGLRTVFDLRGADEAADRPDQLPEGVERVPLDVLADMSRTGPVLLGQLL
                     ESVALANEVLGDGKVDRHFDGAYRGFVTFASARRAYRELFLGLSDPARLPALFHCLTG
                     KDRTGWAAAALLALLDVPHEVIVSDYLRSNEFTLPRYSTAIDAFVAAGGDRSIPQAVF
                     GVKAQYLEKAFDEVAKRHGTIEAYFEQGLGIDRQGQERLREILLAGA"
BASE COUNT         1846 a         4006 c         3934 g         1834 t
ORIGIN      
        1 ccggatgctg cgtggtgttc tgcagcggga cgacccattg cccttccagc ccccgcgcct
       61 tcgctgtctc ggcggcggcc tggatctcgt ccttggtcag gccggcgagg tcggccgggt
      121 tcgaggtgac cagggcgcca gccttggtgg ccgccatcag catgttggtg aacttcgccg
      181 cgagcgccgc gtcctcctcg ttcagcgcct tcagcttcgt cttgtcctcg tccgacagct
      241 tggcaccggc gcggacgaac cgccggtagt agtactcggc cagccgcagg tcctgccccg
      301 ccagcttcag cgcgtcgcgc tgcccccaca gcttctccac gcgctcgaac agcttcccgt
      361 tcaggaagat ggcgtcgtcc agggcggcaa gcttcggggc gatctcttcc tgcagagctt
      421 gcagcgtcgg gttcgtgttc gccgacgaca ggccgttgaa ggcgaggttg acgcgagtga
      481 gcagctggcc gctccgctcg agcgccacca gtgtgttctc gaaggtgggc ggctcggcgc
      541 tgtcggcgat ggctgcgatt tcgtcgagct gctggcggat gccggcctcg aaggcgggct
      601 tgaagtcggc gtcctggatc ctgtcgaacg gcggcgcgcc gtacggcagg ggactgggcg
      661 tgagcagcgg gttggcccgc tcggcggcgg gcgcctggtt ctcggtcatg ggttcgggag
      721 ctgaggaata ggaggccagc gcgaacgcgc cggcaatgcc agccgcaagg acgacgagga
      781 tgagcatggc gcgtcgaagc atgagatgag tcctcccggc gacggatgac tgagcggctt
      841 aatgacggct tgatgatcat agcgcgatgc cgatcgaggg cgcgagcgcg cggcaggccg
      901 accttgcgat ggaaggcaca aacagccttg tgatggacgg cacaagcaga tgttgccttt
      961 cccgaactac ctgacaaagg tcgccctcac ggcggcaatc tggagccttc gtcgtgctgc
     1021 cgcgaacgca acaagctctt ttgcttgaat agttacgtga cgggtgggtc agcgaggtcc
     1081 gactggccca aggcttgcca gcgctgcgac atctgctgga tccaattggt gggctcctca
     1141 acgatgcacc tcttaggcaa tctggtctga cggttcaccc ctgatctttc ccgagcgtgg
     1201 gagccgtcag cattggcagt gtcggccggg gcagtcggtc cgtggctcgt tgcggtgctg
     1261 gcggcaaggc aagctactcg tgtgccttgc cgcaggtgtc cgtggcgccg cgattggctg
     1321 tcatggttcc ccttcctggc cggcggcctg cgcgcggctt gggctcgacc ccattcgctt
     1381 ggggtgccct ggccgcgacg gctcagcccc tagtgtgtgg gactgacggg gcggtagctt
     1441 gtccgatctt ctcgcttgtc cggtcttccc ccgcgacaac tcaggaagta tcgagccatg
     1501 cagatcctgg tcgccacggc cctcgcgctg ttgttctgtg cacaaggcgg cgcgctgacg
     1561 caggccaacc cggcacagtc gaagcagccg gcaccgggtg cgcgggccgc cgaggccgtg
     1621 gctccccccg cgcacccggt tgatccgact gtggccgcgg acgcgcccga ccgcggcttc
     1681 accatcggcc ccgaggacgt gctcgggatc ctcgtctggc gggagcccga ggtgtcgggg
     1741 gacgttaccg tccgtcccga cggcatgatc acgctgccgc tgatccgcga cgtgaaggcc
     1801 gccggcctgg cgcccgatgc gctggccgac cgcatccagg cggcgctgcg cgagtacatc
     1861 accgacgcgt cggtcacggt cgtcgtccgg cagatgaaca gccgcaaggt gttcatcacg
     1921 ggcgaggtgg tgcggcctgg cagctatccg ctggcgtcga ggatgaccgt gatgcagctc
     1981 atcgcggtcg caggaggcct gaccgagtgg gccaacgcca ggaagatctc gctcatcagg
     2041 gtggagggcg gcaagacgac cacccacccg ttcgattacc aggatgtggc gcagggcagg
     2101 aagcctcagc agaacctcgt gctgagggca ggggatacgg tggtcgtacc ggaggcctga
     2161 gccggtgctc gtggcgcagc caatgacgtg ctcccgcagc gcaggtgtgc gctgtgcggc
     2221 ggcgcttgcg ctggtggtat ggctggcgat gcccgcctgg ggccagtcgc cccagcagcc
     2281 ccaggcaccg ccgccggccc cgccacaagc ggcgccggcc ggccggcaga cgccggagca
     2341 gcaggcagag ccggtgccgg ccgagcgcga gaggcgccgg ggacgaacca ccgagtcacg
     2401 tcgggagccg cggcggggcg cgacacggat gcgatcccag gaggagcgca cgtcgcgcga
     2461 ccccgggcta acccgccggg aactgacgct cgccgcgaac ctgaatggcg gctacgacga
     2521 caacctgacc gggggtctgg gcgggggcgc gggaacggcg ccgcggatca tggccagcgg
     2581 atcgaccggc tacttcgacg ccgcgctcgg ctacttctac ggaaacgcct tgcgctcggt
     2641 acgcgtggac gccgccggca gcctccaggc atacccggac tatctggacg gcgtggcgcc
     2701 aggaggcgcg gccacgatct cggcgcagac gaacctgggc cggagaaccc tgttcagggc
     2761 ttccgagcgc gtcggctacg agccgctgtt cagtgtcttc tcccctggcg cgagcagcgc
     2821 gccgctgcca gggggcatcg cggagacggc gcccgcggcc ggactctacg agcgccgctc
     2881 ggccagctcg aacgcgctgc tggcactcga ccgccagtgg ggccgccgcg acgcgacctc
     2941 tttctcctac atgtacaggg tccagcgctt caccagcgag gactacggcg acaacagcgc
     3001 gcaggatctg ggcgccgagc accgccgcac gttggcccgg ggcgtcggcc tccgggcggc
     3061 gtaccggcac gttcgccgcg attacaccga cttcgccggc gagggacggg cgacgcggga
     3121 gcacaggatc gagggcggtc ccgagatcga gtacacccgt tcgcgccgcc gaagcctgag
     3181 cctgctgctg gctggcgggg ccgcgcacgt cgagtcccta agctccgccg accgcccgtc
     3241 gtactcgtcc tgggtgccca tcgggagcgg aacgctgacg ctcgggctgc cgccgaactg
     3301 gtcgctgcag ggcgggtacc agcgcgactt cgcgctgctg cggggcgtga ccgacgaggt
     3361 ctacacgacc gacaccgcct tcggcagcgc cggcggcctg ctttcgtcgc gcgtcgatct
     3421 tcggctgggc gggtcgttca gccgttggag gacgtcgctg gtgcccggtc tcgacggccg
     3481 gctcaggacg tacggcgggt cgctgcagct cggcatcgcg ctgaccggcg ccgtcgcggc
     3541 cacggccggc taccactact atcaccatcg ctactccgac ccggccgcgc tccccgaggg
     3601 gttcccggcg aggtacaacc gtcacgccgt ccgtgtcggc atcgccgtgt gggtcccact
     3661 gatgggcgcg atttcgcggc ccaccagaat cttgcgatag gtagcaggcg aacgatcatg
     3721 attcctgggc aggacctaac gatcaagttc gcgatcaggg cgctcaaaag gcggccgtgg
     3781 ctcgtcgccg gtcccctgtt cttcggcgtg atgggtgggc tcctgtactc ccgctcgcag
     3841 ccgagcctct accgctccga cgccgtggtc caggtcgtgc cgcagcgaat ccccgacacc
     3901 tacgtccagt ccaccgtcac cgcgcgcgtg gaggaccgcc tctcgtcgat cgcgcagcag
     3961 gtacttagtc ggacccagct cgagtcggtc atcctcgagt tcgacctcta cccggatgaa
     4021 cgccgccggt tcccgatgga ggacgtgttc gagctgatga ccgagcgggt caatgtggcc
     4081 ccgctcgagg ggtcaacacg aggccggcaa caggcggtgg atgcgttccg catctcattc
     4141 gactacgagc gtcccgaggt cgctcgggac gtggccgccc ggctcgcggg cttcttcatc
     4201 gatcagaacg tcaaggagcg gggttcgcag gccgatcaga ccagcgcgtt cctcgagtcg
     4261 cagctggccg acgcgcggtc tcgcctcgag gcgcaggaga agaagctcga aaccttccgc
     4321 gagcgcaact cggggcgcct tccgacgcag acgcagacca acatgcaggc gatccagaac
     4381 acccagatgg cgctccaggc catggtggag tcgctggcgc gggacaggga tcgcaagctg
     4441 atgctcgagc gcctctacac cgacgcggcg gccgactccc ccgttgtggc cccggccacc
     4501 ggaccggttc cgtcccccgc accggccgcg actgaccagg cgagtgtcct gcccggggcg
     4561 ccgccgcggc agcggctcga agcggcgcgc gccgcgctcg cgcagatgga ggttcgcctg
     4621 agtcagaagc acccggacgt gatccgcacc aagcggctca tcgatgatct cgaacggcag
     4681 gtcgccacgg aagaagctca gcggcccctg tcacccgatg cggcgtcgga acgccctgcg
     4741 agccaggagg agttgcggcg ccgcgacagg ctgcgcgaga tgcgcgcgga gattgagagc
     4801 ctcgatcgac agatcgcctt caaggagagc gaggaaagtc gcctgcggaa cgagatcggc
     4861 gcctaccagg ctcgcctgga agccgtgccg ggtatcgagt cggagtggat tgccctgacc
     4921 cgcgactacg acacgctgca ggacacctac aagcaactgc tcgccaagag cgagaactcg
     4981 aagatggcgg ccagcctcga gcagcgccag atcggggagc agtttcgcat cctcgacccg
     5041 ccacgggtgc cggtgaagcc ttacagcccg aaccgcatga agatcaatct gctcggcacg
     5101 ctcgccgggc ttggtctcgg gctgctcctc attgggctgg cggagtaccg cgacaccacg
     5161 atgcggaccg agtcggacct catgggcgcg atggatctgc cggtcctcgg gctgctgccg
     5221 tacgttcaaa ctgaagccga cggccggcgg ctgcgtatcc ggcgccggtt tctagcggcc
     5281 ggagtgctgg gggttgccat tgccacggga accgccttct gggtcctcga actgtggaag
     5341 tacgtcgtat gagcgggaca ggtgtggagt cctgatgggc cgaatcgacg aagcctaccg
     5401 ccgcttgaac ctcgatgtgc gccggggcac gggcgccccc gcctcggacg gagacggctc
     5461 accctggcag ccggactcgc tcgggggagg cgacgggcgg caggcggcca tgccggccca
     5521 gcccagccag accgcgcccg cgcccgcctt gcgctggaag caacagggcc acgaaggaag
     5581 cgagcgccgc gaccgctggg cgaggctgga cgtcgaggcg acgcgacggc tcgtcgtgtc
     5641 ggaccaggct ccgccgctgc tcatcgagca gtaccgaagc ctcgccgcga ccctgcttgc
     5701 cgcaccagga gaacggccac ccaggagcct catcgtcacg tcggcctccc cgggcgacgg
     5761 gaagagtcac gtcgccgtga acctggccct cacactggcc cgctcctacg gacggcaggt
     5821 cctgctgatc gatgccgacc tgcgccgtcc ggtcctgaac cgcatggtag gcgcgccaaa
     5881 cagccgcggt ctggccgagg ccctggaggc agaagcggac gagcggttgg cgaccgtacc
     5941 aatcgaggag ggcctgacgc tcctgccggc cgggcggccg acaccgaacc acattgccgg
     6001 cctcgcctct gaacggatga agaggatcgt ggacgaagca accgcacgat tcgactgggt
     6061 gatcgtggac acgccgccgg ttggcatcct cgcggacggc cgcctggtcg cggatatggt
     6121 ggacggtgtg ctgctcgtcg tccgggcggg agaaacgcca ttccctgacc tcgaggcggc
     6181 ggtcgatgtc atcggtcgcg agcggatcct ggggatggtg ctcaacgccg ttgaccccgc
     6241 agagattcgc ggcagggagt actaccaccg ctactaccac ggccacgagg ctcgcaaggg
     6301 ttgagtcgcc ctcctgcatt tcctaaccga ccgctgaccc cgatgtcccg cctggctcac
     6361 gacgaaccat gcgctgcgct cgaatgcacg ccgggcgtgg agtacgacga gggaaccttc
     6421 cgctacttcc tcagtgtcga gcgggcccgg gccgagcggt ccaaccacgc gctccgtgtc
     6481 gtgttcgcga gcatcgagct ggtccctggc gagcctgccc gtatctcgcg cgctcgggcc
     6541 gccgagttgt tcgaagggtt gcgccggtcc ttgcgcgaca ccgacatcac cggttggtac
     6601 aggcaggacc gggtggcagc cgctctcttg agcgagcggg ccgaaccgcc cggtgccaac
     6661 acgcttggcg tcatcgaacg acgcgtcgat gaaggagttc gccagcgcct gccgcggaga
     6721 atcgcacgcg ccctgaaggt gcgagtggtt cagctgaggc cgcggcgggc caggaatgaa
     6781 gaggagcttc cagtgtgaac ggtgcatcgc ctccccgtac cgagccgctg tccacctctc
     6841 ccggccgacg gcttgccgcc ctttgggcga tcccctcgcc ccctgcgagg gcggtgggaa
     6901 ttcggcagcg ccacccgatc ctcggcgaat ctctcttccg cgaggcgatc ctgcgtgagc
     6961 agaggcgggc cgatcgtttt gacacgccat tcgccctgct gttgctcgcg ttggccaacg
     7021 gctccggtca agcagactcc tgggcgccgg tcctgcgcgc tgtatctgcc acgaagcgcg
     7081 acaccgacct ggccggctgg cttcacgatg ggagggtgct ggggctgctc ctccccgacg
     7141 cctcggcaaa gagcgcgctt caaaccgagc accgtctgcg ccgcgagatg tccaggcgcc
     7201 tcgacgagcc ccagctctcc gccctgtccg tccatcccta ttcacttggc gaccgctgcg
     7261 gcacggatcc gctgcctccg atcgagttgg tagtcgaaac cttcgcgagc tcgcgcgacc
     7321 tctcgtggcg cgacattgcc aagagggcac tcgacgtggc aggcagcctg gcgttcctgg
     7381 ttctcttctc gccagtcttc gcggttgtct gggcggcggt gaggtggaca ttcgacgggc
     7441 ccgccattta cagacaggtg cggattggcc ggggaggcga ggcgttcaca atgctcaagt
     7501 tccggacgat gtacgtgaac gctgaggacg gtatccaccg cgactacgtc agctggttca
     7561 tcaagtcgag cacccggcag ccgcacgcag cacacgaggt attcaagctg acggacgacc
     7621 ctcgcatcac gccgctcgga cgctttctcc gaaggaccag cctcgacgag ctgccgcagt
     7681 tctggaacgt gctgcggggc gagatgtcgc tggtcggacc gcggcccccg ctgccgttcg
     7741 aggtcgagca gtacaagccg tggcaccgcc gccgcgtgct ggaagcgaag cccggcgtga
     7801 cgggaccgtg gcaagtgcgc gggcgcagcc agacgacgtt cgacgagatg gttcgcatcg
     7861 accttcacta cgccaggacc cactctttgt ggaccgacat caagatcctg gcggccacac
     7921 cacgggccat gctgtcggga aagggcgctt gctgatggac acttacctgg cgattgctcc
     7981 ggacgtgaag ttgggcaagg acgtgaagct ggcccggttc atcaacctct acggctgcga
     8041 gatcggcgac gagaccaaga ttggcgcgtt cgtcgagatc cagaagaacg cgaaggtcgg
     8101 ccgtcggtgc aagatctcga gccacacctt catctgcgag ggcgtgacca tcgaggacaa
     8161 cgtcttcatc ggccacggcg tcaccttcat caacgacacc tacccccgcg cgaccacgga
     8221 aggccttgtc ggcgcgggcg agcgcgtcgc gagcccggcc cgcggcgagc agcatcgacc
     8281 cggcccgcat ctgggcctcg gcgtcccgag gcaggagatc ggccgcccgc accgactcgg
     8341 ccagcgcgcc cggaccgttc ccctggcgca tgaaggcctc ggccagcttg agccgcgccg
     8401 gtgccagcat cgggtctgct tccaccgccc tgcggtactc caggacggcg gcgtccacgt
     8461 tccccttcgc gagctggccg tcgccccgct ccacgaagtc ggccgcgctt ctcgagcagc
     8521 cggccaccgc cgccgcgacg aggatcaccc ccacgaacga tcgagctcgc agtgagatct
     8581 tcacggggat cgcgcctcca gcgcggagga tagcaccaat cccgggaggc cgcgcgggtg
     8641 cgcgcaacgg ccccaagctc cttttccgga cgacaccacc gaaggccgac atcgccccga
     8701 ggggcgatgt cggagaacct tacagtgccg aatttgaaca tcgcaccgtg gccggcgatt
     8761 tccagatcct ggtcaccaac ggcgacggtc tcacggggct cccggacagc ttttacgcgt
     8821 tctgcgtcga cctccagcac tgggccgaca cgtcggctgg agcgacgctg ggcagcatgg
     8881 ccggctggga cctgctggac ggcgtgacgg actaccagag gctgggtgcc tcgtacctgg
     8941 ccaaccagta cttctctctc taccccgacg gatcggcgag caacctgacc gccgcttact
     9001 accaggtggc catctgggag ctgctgcacg agcgcaaccc gctcaaggtg acgccgttca
     9061 acgtctggag cgtcaccggc ggtagcacgt acttcagcag cggaacgctc acggctggcg
     9121 cacaagatct catccggggg atcgatacca gctccccact gttcacgtac gccggccacc
     9181 tgatcacgcc cgatcacgac gggtacgggc agaacttcat cgcgccggtg cccacccccg
     9241 ttcctgacgg cggcacgacc ctcatgctgc tcggcggcgc gctgattggc ctcgacctcc
     9301 ttcgccggag gctgagaccg tagtgcggat cctcgcctga cgggcccgcg tcccccatcg
     9361 ctcacgggaa cgcccgcgcg gcgtcgcgtg tattgtcttg cgatgatgcc gtcatcgttc
     9421 catgcaggac atcccgggaa cgcgcctgtg ccaagacggc acacaaccca cgtaaccaac
     9481 tgagccattg gaactaaccg gacgccgcat tcgcgacacc gtaacgcgag gtcccttgag
     9541 cgcccatggc gcgacaagtg ctttggagct tgtcggcacc gtggccatca gcgcaccgcg
     9601 cggctccggg cgccgtccac acgcgtcaca gggagggcct ttcgtgaaga gacgtcacct
     9661 gctcggactg atcgtcgccg cgagtacgtt gatgccgcca gcggccggcg cggaacccat
     9721 caccttcttc gggttcgaca gcggcctggg cgaactcgcg cgactcagct cctctcccaa
     9781 cgcggacgcg gcgcgtgccg cgttcatggc caacctggtt ggtgccggca ccgaggactt
     9841 cgaaagttac gccgccggaa cgacggcacc gctggccatc gcgttcccgg gcgcaggcac
     9901 cgccaccttc ttcggcgacg gctccatcgc gagccagccg tcgggcacca acggtttcgg
     9961 gcggtacccg gtgtctggaa gccagttctg ggaccggcag gaggcgttct ccatcgcgtt
    10021 ctccgaaccg attgccgcct tcgggttcta cggggtggac atcggcgact tcggggggcg
    10081 catgagactg tggctcgccg acggcggctc gacgtacctc gacgtcccgc acgacatcga
    10141 ccaggccggc ggcagcgtca tgtacttcgg cttctacgat ctcgaccagc agttcgtcgg
    10201 catcaccttc ggcgacaact acgcgtcggg aaccgacttc ttcggcctcg acgacctgac
    10261 ggtggcgtcg cgcgcgcagg tgcacccggt gcccgagccg gcgtcggcgc tgctggtcgg
    10321 ccttggcctt gccgggctgg cagctgtccg caggcgccgc gaataggccc cacctcgacg
    10381 cacttcacga gggccgccgc gaggcggccc ttcttgtctc tggaggcacc tggcctgccg
    10441 ccttggcccc ctcgcttcgc cggcctcgtt tgcgtgggga gccgcccgtg tgtgtaatag
    10501 ccggatgact ggcggcgcaa ccctcgacat cccctcgctc cccaacctcc gtgaccttgg
    10561 cggacaccgc acggcggacg ggcgcatcgt gcgcaccggc ctcgtctacc gatcggacca
    10621 gcccagcccc atgacggcgg aagacgtcga gcgcctcgcg acgctcggcc tgcgaaccgt
    10681 attcgacctg cggggagcag acgaggccgc cgatcgaccc gaccagttgc ccgagggcgt
    10741 cgagcgcgtg ccgctcgacg tgcttgccga catgagccgg acggggccgg tcctcctggg
    10801 gcagctcctc gaaagcgtcg cgctggccaa cgaggtgctc ggtgacggca aggtggaccg
    10861 ccacttcgac ggcgcttacc gcggcttcgt gaccttcgcc agcgcaaggc gggcctaccg
    10921 cgaactgttc ctgggcctgt ccgacccggc gcgcctgccc gccctcttcc actgcctcac
    10981 cggcaaggac cggaccggct gggcggccgc cgcgctgctc gcactgctcg acgttcctca
    11041 cgaggtcatc gtctccgact accttcggag caacgagttc actttgcccc gctacagcac
    11101 ggcaatcgat gccttcgtcg cggccggcgg cgatcggtcg atcccccagg cggtcttcgg
    11161 cgtgaaggcg cagtacctcg agaaggcgtt cgacgaggtg gcgaagcgcc acgggacgat
    11221 cgaggcgtac ttcgagcagg gcctggggat tgaccggcag ggacaggagc ggctgaggga
    11281 gatcctgctg gcaggcgcct gagggcagtc tgtggcgcac agggtcccgc atgccaggga
    11341 cagacgggac gcctgtgccg cacactcacc caccgggcac aggcgggacg cctgtgctac
    11401 tgcacacagg cgagacgcct gtgctactgc acacaggcga gacgcctgtg ctactgcaca
    11461 caggcgggac gcctgtgcta ctagaccagc aacccccggg gactgctgcc gccgccatcc
    11521 aggatcgagc gcgcggacct ggggtcgtcg tagtaatcgc tcgaggagct gctcgaactg
    11581 ctcgaccggc tcgagctgct ggaagaactg ctcgaactgc
//
LOCUS       DAOPQX010000085        11592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6414_length_11592_cov_4.340903, whole genome shotgun sequence.
ACCESSION   DAOPQX010000085 DAOPQX010000000
VERSION     DAOPQX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6414_length_11592_cov_4.340903"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PKK95_06550"
     CDS             complement(<1..1051)
                     /locus_tag="PKK95_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17906.1"
                     /translation="MPEISALQKFTCPACGAQAEWNPAKQGLICPFCGTESPYQADPS
                     TGAIVETDLVAALRELSDDQRGWQAERRSVQCQSCKAVMVYDPTRVGQNCEFCGSPAL
                     VPYEEIKAPIRPQSLLPFKVARTAVHDSMRQWYAGRWFAPNALKRRALVDQIKGLYIP
                     YWTFDARVHCPWTAEAGHYYYVQERVRDNQGKWQTRQVRKVRWEPASGTVDHFFDDEP
                     VPGTKGVQRDLLKAVEPFPTKDLVPYDASYLSGYVVEHYQVVLVDAARQSRDSMHEQL
                     RTMCARQVPGDTHRNLRIFPSYSGETFKHILVPIWLLTYTYGSRAYQVVANGYTGRLA
                     GRYPKSGWKIAGAVLL"
     gene            complement(1131..2198)
                     /locus_tag="PKK95_06555"
     CDS             complement(1131..2198)
                     /locus_tag="PKK95_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOC17907.1"
                     /translation="MGVFDFIKGELLEIIEWTDDSRDTLSFRFPDDDKAIKNGAQLIV
                     RESQVVQFVYLGEFGDTFGPGKHTLTTDNIPILTRLKSWKYGFNSPFKADVYYVSTRL
                     FTGNKWGTANPIMVRDEDFGVVRLRAFGTYDFKVIEPKLFLREVAGSDQNFRLDEFAD
                     TMRSRIVSVFSDALASSRVPALDVAARFMELGEALLPVINPIVGAKYGLEISSFIVEN
                     VSVPPEVEQAIDKRSSMAVVGNLNDFVKYQMAKGMETGGAGGAGMAAELAVGFGIAQQ
                     MMQQGLGPQQAGHAAAVGAAQAATAPAAADLLTPADAARILGVPEADVMAVIESGELK
                     AKKIGSSYRITRAAFDAYLHS"
     gene            complement(2234..3808)
                     /locus_tag="PKK95_06560"
     CDS             complement(2234..3808)
                     /locus_tag="PKK95_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOC17908.1"
                     /translation="MRYFEPWTYRSWPKPVVGWCSWFAFYDKVTEQDVKRVADVMSEE
                     LLRYGYDYLQIDDGYQRGTGLPELWLKANEKFPSGLPELARYIRGKGLKPGIWTNAAF
                     TQTDFAARHKPWFVAGPDGQPARGNWIGHVVDATAPGALDALVRPIYKDLRAQGWDYF
                     KLDALRHLRYEGYNTFRAHFEGRPEGPTEALRAYVASVRDEIGRDSFLLACWGIRPEL
                     VGLVDGCRIGTDGFSYAGLAQFNSFNNVVWRNDPDHIELSDTEAWRSTMVTSLTGSMF
                     LLTDKPERYRTAVADPARRAAPVLVTTPGQLYDVDPSRSSQLWRVDGEVSGRDPKPFD
                     ASLTPSVFLYQLDIHRPFGSWTVVGRTGGSFDAIPFEDLGLDPAKRYLVFEFWQRRLL
                     GAFERQFAPGPLPTPFNSQVFIVREQRPGPQFLASSRHITGGGYDLESLEWADGVMTG
                     RSLAVAGEPYEVYLTEPDGWTLQGLECDGGLPLAVERGNGWAKGGCTPKSGGALAWRA
                     RFRPGLPPPAPRLPLQ"
     gene            complement(3693..4424)
                     /locus_tag="PKK95_06565"
     CDS             complement(3693..4424)
                     /locus_tag="PKK95_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17909.1"
                     /translation="MLKGVSSVFVLILGAAFFGSAVISSQPGLASRAAAGPVQAPPNE
                     AAQLSLDGGRVQLRYHDTLVFDGRVTNPSALRAIRPSTSKTGDRVDQVLAFFASGSEP
                     IELSGTITASGEAFACEADRPLRGRPIVRHVSGDGSSLLDRAVYDRHADWVLSGGRPA
                     AHPDAGDDGRGDAQLAGWRSHLHPRGARRRDPPPLPASLLPAAPGPAVLRAVDLPLVA
                     EARRRLVLVVCFLRQGHRAGREARR"
     gene            complement(4465..5100)
                     /gene="queE"
                     /locus_tag="PKK95_06570"
     CDS             complement(4465..5100)
                     /gene="queE"
                     /locus_tag="PKK95_06570"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HOC17910.1"
                     /translation="MKYRVKEIFYSLQGEGANTGRPAVFCRFSGCNLWSGFEVDREKA
                     ICRFCDTDFVGTDGPGGGVYEDPSALADAVAAKWPAASAIRPFLVCTGGEPLLQLDGA
                     LLERFARHDFEVAVETNGTRPLPGRLDWVTVSPKAGSRLVLASGDELKLVYPQQGLDP
                     AAFEGLAFRHFFLQPMDGPDRWRNTLLAMEYCADHPRWRLSIQLHKYLGLR"
     gene            5260..6231
                     /gene="cysK"
                     /locus_tag="PKK95_06575"
     CDS             5260..6231
                     /gene="cysK"
                     /locus_tag="PKK95_06575"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOC17911.1"
                     /translation="MSRIYDDNSLSIGRTPLVRLNRITAGLPATVVAKIEGRNPSYSV
                     KCRIGAAMIWAAERDGILRREVPDRIRVVEPTSGNTGIALAYVCAARGYPLVLTMPET
                     MSLERRRMLKAYGAELVLTEGAKGMKGAIARAEEIVASDPARHWMPQQFTNPANSDIH
                     FRTTGPEIWDDTDGKADILISGIGTGGTITGVSWYFEREKQKPLWGVAVEPVESAILT
                     AMRAGLPFQPTPHKIQGIGAGFKPEVLDLDAVDEIQTVSSDEAIEMARRLHREEGITG
                     GISCGAAAVAAVRVAARAENQGKLVVVILPDAGDRYHSSALFQGIEV"
     gene            complement(6241..7260)
                     /locus_tag="PKK95_06580"
     CDS             complement(6241..7260)
                     /locus_tag="PKK95_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17912.1"
                     /translation="MPSLQLILVLLAAVTAAAVAPQVPRHRERVEVSRVLVDARVLDG
                     RGRAVTGLGPGDFDVRIDGQRARVESAYWVGGDEAPPEAAAPGTPPPSRPEEARGRLI
                     VFLYQKDLEPSRIVGLMRMLIETRTFLDSLGPDDRVAVLSFDSHLKIWLDFTRDIDRV
                     REVLRRGILFRDPGPVEPVAGPSLMATLDVVRARRASSMEDALALLGRALEPIPRAKS
                     VVLVGHGFGRLFGSSILFEGTYGEARRSLQAARASVFALDVTNADAHTLEAGLQMTAR
                     DTGGFYERTHLFTKAAVNRLAGALSGHYVLFVEVEAPLDRERHEIDVRLARGVEGTVF
                     AKRGY"
     gene            complement(7282..9390)
                     /locus_tag="PKK95_06585"
     CDS             complement(7282..9390)
                     /locus_tag="PKK95_06585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOC17913.1"
                     /translation="MTRRFLPLFVVLLLIMTAAAGRAQEAAAGNPLLKEWKTPFEVPP
                     FQEIQAEQFLPAFKVAIAENRKEIEAIAGNPQAPTFANTVEALERAGVLLGRVSGVFG
                     ALQGAETNPRLQAINREVTPMLSALRDEIRLNEKLFARVKAVYEARESSGLTAIQKKL
                     VEDTYKGYVRSGASLDAAKKDQLRKINAELSMLSVRFGDNLLHDTNDFKLVIDRKEDL
                     AGLPASVVAMGADAAKAAGLPGKWVYTLHAPSIWPFLQYADNRELRRRLLDAYLTRGD
                     HGDQYDNKQIVSRIAALRVERANLLGYKSHADFVLEENMAKTPAGVYGLLNQLWAPAR
                     EMALKEAAVLQEQIKAEGGDFTLAPGDWRYYTEKVRKAKYDLDEQQLRPYFQLEQVQQ
                     GAFYVANRLYGITFTPRPDLPVYHPEVKAWEVKDADGTHLSIFYTDYHPRPGKRGGAW
                     SGRMRGSWVRDGKSVRPIATNVCNFSRPAGDEPALLTLEEVGTLFHELGHAIATFLNK
                     VPYTGLGGVPRDFVEVPSQIMENWALEPEVLRVYAKHYRTGEVIPTDLVEKIRESAQF
                     NQGFITVEYLAASMLDMDWHTLTAPTGQDAAAFEKASMEKIGNPPAIPPRYRSPYYSH
                     IFAGGYSAGYYAYIWSEVLDKDAFQAFKETGLFDQATAKRFRTEILEKGGSEDAMTMY
                     KRFRGKEPGVDALLKARGLK"
     gene            9627..>11592
                     /locus_tag="PKK95_06590"
     CDS             9627..>11592
                     /locus_tag="PKK95_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC17914.1"
                     /translation="MTTVLVLALGIGVNTAVFTAINGLLFEPLGGPAAGELAAIFARD
                     ARRDGTYRPLSPADYRELAAASRPSGVFTSLMAHQMLTVGVTEAGRTRKAYACLASST
                     YFQTLGIAPPVGRAFLPAEDDPSAAPVAVVSRAYWERNGSEAALGRRITINNHAFTIV
                     GVVPEGFSGTTALLSPELWLPLGAAPLLRAHERHDASGDPDRMPALMAVARLRPELSL
                     EAAGPLVASFAPSLRQAAPVENADLELSVGPLSRFSMGAGPRDDTEAAGVLFFLMGMA
                     SVVLLIACLNLANLLLARGSARSREIAVRLAVGGSRGRVVRQLLAESLLLSLAGAGVG
                     VAVSAGGTALLFRTLSAVLPLPVVFDATPDPRTLAAAAGLATLATLAFGLGPAMNLAR
                     RDLLPALRERQSHQPAGRFGLRARNLLAVGQLALCLALLTAAGLFVKGAWEAGDADPG
                     FRLDRGIVGSSDASLAGYDELRGREAYRRVVARLRRLPGIESASLASAVPLGLEGSYR
                     EVKPVGGTGSSEAAWASTVVVGAGYFGTVGLPLLRGRDFTPAEEESASPLRPVLVDLP
                     MARRLWPDLDPLGRHIQLEVEGGGGTAWSEPFEVVGVVPGVRDSLFDKEPAAHVYLPF
                     GAEYRAAMYVHARLATSDPEATEAILSAMRR"
BASE COUNT         1721 a         3991 c         4075 g         1805 t
ORIGIN      
        1 cgagcagcac cgcgccggcg atcttccagc cgctcttcgg gtagcggccg gcgaggcggc
       61 ccgtgtaccc gttggccacc acctggtagg cgcgcgaacc gtaggtgtag gtcagcagcc
      121 agatcggcac caggatgtgc ttgaaggtct cgcccgagta cgaggggaag atcctcaggt
      181 tgcggtgggt gtcgcccggc acctgccgcg cgcacatggt ccgcagctgc tcgtgcatcg
      241 agtcgcgcga ctgccgcgcg gcgtccacca gcaccacctg gtagtgctcc accacgtagc
      301 ccgagaggta ggacgcgtcg tacgggacga ggtccttggt cgggaacggc tcgaccgctt
      361 tcagcaggtc gcgctgcacc cccttggtcc ccggaaccgg ctcgtcgtcg aagaagtgat
      421 cgaccgtgcc cgaggccggc tcccagcgca ccttccgcac ctggcgcgtc tgccacttcc
      481 cctggttgtc gcggacgcgt tcctggacgt agtagtagtg cccggcctcg gcggtccagg
      541 ggcagtgcac gcgggcgtcg aaggtccagt aggggatgta gaggcccttg atctggtcca
      601 ccagcgcgcg ccgcttcagg gcgttggggg cgaaccaccg cccggcgtac cactggcgca
      661 tcgaatcgtg aaccgcggtg cgcgccacct tgaagggaag caggctctgc gggcggatgg
      721 gagccttgat ctcctcgtac ggcaccaggg ccggcgagcc gcagaactcg cagttctggc
      781 cgacccgcgt cgggtcgtac accatcacgg ccttgcacga ctggcactgc acgctgcggc
      841 gctccgcctg ccagccgcgc tggtcgtccg acagctcgcg cagcgccgcc acgaggtctg
      901 tctcgacgat ggcgccggtc gaggggtcgg cctggtaggg cgactcggtc ccgcagaacg
      961 ggcagatgag cccctgcttg gcggggttcc actcggcctg cgccccgcag gccgggcagg
     1021 tgaacttctg aagcgcggag atttcgggca tcaggagtcg agctcagggc cactctttgc
     1081 cttcgccggc agcgggcggg agcgaacgcc cgcccggccg gcgaccggtg tcagctatgc
     1141 aggtaggcgt cgaaggcggc gcgcgtgatc cggtagctgc tgccgatctt cttcgccttc
     1201 agctcgcccg actcgatgac cgccatcacg tcggcctcgg ggacgccgag gatccgcgcc
     1261 gcatcggcgg gggtcagcag gtcggccgcg gccggcgcgg tggccgcctg ggccgccccg
     1321 acggcggcgg cgtgcccggc ctgctgcggc ccgagcccct gctgcatcat ctgctgggcg
     1381 atgccgaagc cgaccgccag ctcggccgcc atgccggccc cgccggcccc gccggtctcc
     1441 atccccttcg ccatctggta cttgacgaag tcgttcaggt tgccgacgac cgccatgctc
     1501 gaccgcttgt cgatcgcctg ctcgacctcg ggcggcaccg acacgttctc gacgatgaag
     1561 ctgctgatct cgaggccgta cttcgcgccg acgatcgggt tgatcaccgg cagcagcgcc
     1621 tcgcccagct ccatgaaccg ggccgccacg tcgagcgccg ggacgcgcga cgaggccagc
     1681 gcgtcgctga acacgctgac gatgcgcgac cgcatcgtgt cggcgaactc gtccaggcgg
     1741 aagttctggt cggagcccgc cacctcccgg agaaacagct tcggctcgat caccttgaag
     1801 tcgtaggtgc cgaaggcgcg cagccggacc accccgaagt cctcgtcgcg gaccatgatc
     1861 gggttcgcgg tcccccactt gttccccgtg aagaggcgcg tgctgacgta gtagacgtcg
     1921 gccttgaacg gggagttgaa gccgtacttc cacgacttca ggcgcgtgag gatcgggatg
     1981 ttgtcggtgg tcagcgtgtg cttgccgggg ccgaacgtgt cgccgaactc accgaggtag
     2041 acgaactgca ccacctgcga ctcgcggacg atcagctggg cgccgttctt gatcgccttg
     2101 tcgtcgtccg ggaagcggaa cgagagggtg tcgcgagagt cgtcggtcca ctcgatgatc
     2161 tcgagcagct cgcccttgat gaagtcgaac acgcccatgg cgaaggctcc ttttcggtga
     2221 agggacggaa agctcattgt agcggaaggc gtggggcggg aggcggaagg ccgggccgga
     2281 atctcgccct ccacgccagc gcgccgcccg acttcggcgt gcagccgccc ttcgcccacc
     2341 cgttgccgcg ctcgaccgcc agtggaaggc cgccgtcgca ctccaggccc tgcagcgtcc
     2401 agccgtcggg ctcggtcagg tagacctcgt acggctcgcc ggcaaccgcg aggctgcggc
     2461 cggtcatcac gccgtccgcc cactcgagcg actcgagatc gtagccgccc ccggtgatgt
     2521 ggcggctcga ggcgaggaac tgcggcccgg gccgctgctc gcgcacgatg aagacctgcg
     2581 agttgaaggg ggtgggaagc gggcccgggg cgaactggcg ctcgaaggcg ccgagcagcc
     2641 ttcgctgcca gaactcgaaa accaggtagc gcttcgccgg gtccaggccg agatcctcga
     2701 aagggatcgc gtcgaacgag ccgcccgttc gcccgaccac ggtccaggag ccgaacggcc
     2761 ggtggatgtc gagctggtag aggaagaccg agggtgtcag gctggcgtcg aacggcttcg
     2821 gatcgcggcc gctcacctcc ccgtccacgc gccagagctg cgacgaccgc gagggatcca
     2881 cgtcgtacag ctgacccggg gtggtgacca gcaccggcgc ggcgcggcgg gcgggatcgg
     2941 ccaccgcggt gcggtagcgc tcgggcttgt cggtgagcag gaacatcgac ccggtgagcg
     3001 aagtgaccat cgtcgaccgc caggcctcgg tgtcgctcag ctcgatgtga tcggggtcgt
     3061 tgcgccagac gacgttgttg aaggagttga actgggcaag gcccgcgtag gagaagccgt
     3121 cggtgccgat gcggcagccg tccaccaggc ccaccagctc gggccggatc ccccagcagg
     3181 cgagcaggaa cgagtcgcgg ccgatctcgt cgcgcaccga cgccacgtag gcgcgcagcg
     3241 cctcggttgg accctctggc cttccctcga agtgcgcgcg gaacgtgttg tagccctcgt
     3301 agcgcaggtg cctcagggcg tcgagcttga agtagtccca gccctgcgcg cgcaggtcct
     3361 tgtagatcgg gcgaaccagg gcgtccagcg cgcccggcgc ggtcgcgtcc acgacgtggc
     3421 caatccagtt gccgcgcgcc ggctggccgt ccgggccggc gacgaaccag ggcttgtgcc
     3481 gcgcggcgaa gtcggtctgc gtgaacgcgg cgttggtcca gatgcccggc ttcaacccct
     3541 tcccgcgaat gtagcgtgcg agctccggca gcccggacgg gaacttttcg ttcgccttga
     3601 gccacagctc cggcagcccc gtcccgcgct ggtagccgtc gtcgatctgg aggtagtcgt
     3661 agccgtagcg cagcagttcc tcggacatca cgtcagcgac gcgcttcacg tcctgctcgg
     3721 tgaccttgtc gtagaaagca aaccacgagc accagccgac gacgggcttc ggccacgagc
     3781 ggtaagtcca cggctcgaag taccgcaggc cccggtgctg ctggtagaag cgaggccgga
     3841 agcggaggag gatctcgccg ccgcgcgcct cgagggtgaa ggtgcgagcg ccagccggcg
     3901 agttgggcgt cgccgcgacc atcgtcaccc gcgtccgggt gcgcggctgg tcgtccaccc
     3961 gagaggaccc agtcggcgtg cctgtcgtag acggcgcggt cgaggaggct cgagccgtca
     4021 cccgaaacgt gccggacgat cggccgtccg cgcagcgggc ggtcggcctc gcaggcgaac
     4081 gcctcgccgc tggccgtaat cgtgcccgag agctcgatcg gctcgctgcc gctcgcgaag
     4141 aaggccagta cctgatccac ccgatcgccc gtcttcgacg tgcttggccg gatggcgcga
     4201 agcgccgatg ggttggtgac gcggccgtcg aagacgaggg tgtcgtgata gcgaagctgg
     4261 acgcggccgc cgtcgaggct gagctgcgcc gcctcgttgg gaggcgcctg gacggggccg
     4321 gcggcggcac gcgacgccag cccgggctgc gacgagatga cggcgctgcc gaagaacgcg
     4381 gcaccgagga tgaggacgaa gaccgacgag acgcccttga gcatggctct agtctagaac
     4441 cggatgggtc ggccgagcgt ccgcctaccg cagcccgagg tacttgtgca gctggatgct
     4501 gaggcgccag cgagggtggt cggcgcagta ttccatggcc agcaaggtgt tgcgccagcg
     4561 gtcggggccg tccatcggct ggaggaaaaa gtggcggaag gccaagccct cgaaggcagc
     4621 ggggtcgagg ccttgctgcg gatagacgag cttcagctcg tctccgctgg ccagaacgag
     4681 gcggcttcca gccttcgggc tgacggtgac ccagtcgagc cggccaggca acggcctcgt
     4741 tccgttggtt tcgacggcta cctcgaagtc gtgccgggcg aaccgctcga ggagagcacc
     4801 gtcgagctgc agcaggggtt cgccccccgt gcacacgagg aacggccgga tggccgaggc
     4861 ggccggccac ttcgccgcca ctgcatcggc cagcgcgctt ggatcctcgt agacgccgcc
     4921 cccggggccg tccgtgccga cgaagtcggt gtcgcagaac cggcagatcg ccttctcgcg
     4981 gtccacctcg aagcccgacc agaggttgca ccccgagaac cggcagaaca cggccggccg
     5041 gccggtgttg gccccctcgc cctgcaggct gtagaagatc tccttgaccc ggtacttcat
     5101 cgcctattga aactgtacac gacaagcgga cagctgacag ctgacagcga acagctgaga
     5161 gccgacggcg gacagcatct ccggagaccc tcaacaactt ggagcggctt caacgtttcc
     5221 agcccagccg ggatcaagtc ttgcttcacc tcttctacta tgtcccgcat ttacgacgac
     5281 aactcgctct ccattggccg gacgccgctc gtccggctca accgcatcac ggccgggctt
     5341 ccagccacgg tcgtcgccaa gatcgaggga cggaacccgt cctactcggt caagtgccga
     5401 atcggcgccg cgatgatctg ggcggccgag cgcgacggca tcctgcgccg cgaagtgccc
     5461 gacaggatcc gggtcgtcga gcccaccagc ggcaacacgg ggatcgcgct cgcctacgtg
     5521 tgcgcggccc ggggctaccc tctggtgctc acgatgcccg agaccatgag cctcgagcgg
     5581 cggaggatgc tcaaggcgta cggcgccgaa ctggtgctca ctgagggtgc caagggcatg
     5641 aagggggcca tcgcccgcgc ggaggagatc gtcgcctcgg atcccgcgcg gcactggatg
     5701 ccgcagcagt tcaccaaccc ggcgaactcc gacatccact tccgcaccac ggggccggag
     5761 atctgggacg acaccgacgg gaaggccgac atcctgatct ctgggatcgg cacgggcggg
     5821 acgatcaccg gcgtcagctg gtacttcgag cgtgagaagc agaagccgtt gtggggggtg
     5881 gccgtcgagc cggtcgaatc ggccatcctg acggcgatgc gcgcgggcct gccgtttcag
     5941 ccgacacccc acaagatcca gggcatcggc gcgggcttca agcccgaggt gctcgacctc
     6001 gacgcggtcg acgagatcca gacggtgtcg agcgacgagg cgatcgagat ggcgcgccgg
     6061 cttcaccgcg aggaggggat cacgggcggg atctcgtgcg gcgcggcggc ggtggcagcc
     6121 gtccgggtgg ccgctcgcgc ggagaaccag ggcaagctcg tcgtcgtcat cctgcccgat
     6181 gccggcgacc gctaccacag ctcggcgctg ttccagggca tcgaggtctg acgccgcgcg
     6241 tcagtatcct cgcttcgcga acaccgttcc ctcgacgccg cgtgccagtc gcacgtcgat
     6301 ctcgtgccgc tcgcgatcga gcggggcctc gacctcgacg aacagcacgt agtgtcccga
     6361 gagcgcgccg gcgaggcggt tgacggccgc cttggtgaag aggtgggtcc gctcgtagaa
     6421 gccgccagtg tcgcgggccg tcatctggag gccggcctcg agcgtgtgcg cgtcggcgtt
     6481 ggtgacgtcg agcgcgaaga ccgacgccct cgccgcctgc agcgagcggc gcgcttcgcc
     6541 gtaggtgccc tcgaaaagga tcgagctgcc gaacagccgg ccgaacccgt ggccgaccag
     6601 caccacggac ttggcgcgcg ggatcggctc gagcgcccgg ccgagcaggg cgagcgcgtc
     6661 ctccatcgac gaggcgcggc gggcacggac gacgtcgagc gtggccatga gagaggggcc
     6721 cgcgaccggc tccacgggcc ccgggtcgcg gaagaggatg ccgcggcgca gcacctcgcg
     6781 cacccggtcg atgtcgcggg tgaagtcgag ccagatcttc aggtgcgagt cgaacgacag
     6841 caccgcgacc cggtcgtccg ggcccagcga gtcgaggaag gtgcgtgtct cgatcagcat
     6901 ccgcatcagg ccgacgatgc gggagggctc gagatccttc tggtaaagga acacgatgag
     6961 tcggccgcgc gcctcctcgg ggcgcgaggg cgggggagtg ccgggcgccg ccgcttcggg
     7021 cggcgcctcg tcgccaccta cccagtaggc cgattcgacg cgcgcccgct gcccgtcgat
     7081 ccggacgtcg aagtcgcccg ggccgagacc cgtcaccgcc cttcccctgc cgtcgaggac
     7141 gcgcgcgtcc accaggaccc gtgacacctc cacgcgctcg cggtggcgcg gcacctgcgg
     7201 cgccaccgcg gccgcagtca ccgcggccag caggacgagg atcagctgga gggaaggcac
     7261 ccgatcattg tagtgccccg tctacttcaa tcccctcgcc ttcagcagcg cgtcgacccc
     7321 gggctccttc ccccggaacc gcttgtacat cgtcatcgcg tcttccgagc cgcccttctc
     7381 gaggatctcc gtccggaagc gcttcgcggt ggcctggtcg aagaggccgg tctccttgaa
     7441 ggcctggaac gcgtccttgt cgagcacctc gctccagatg taggcgtagt agcccgccga
     7501 gtagccgccc gcgaagatgt ggctgtagta cgggctgcgg tagcgcggcg ggatggcggg
     7561 cgggttgccg atcttctcca tcgaggcctt ctcgaacgcg gcggcgtcct gccccgtggg
     7621 ggccgtcagc gtgtgccaat ccatgtcgag catcgacgcg gcaaggtact cgacggtgat
     7681 gaacccctgg ttgaattgcg ccgactcgcg gatcttctcg accagatccg tagggatcac
     7741 ttcgcccgtc ctgtaatgct tcgcgtagac cctcagcact tccggctcga gcgcccagtt
     7801 ctccatgatc tgcgacggca cctcgacgaa atcgcgcggc accccgccga gcccggtgta
     7861 gggcaccttg ttcaggaagg tcgcgatggc atggcccagc tcgtggaaca gcgtcccgac
     7921 ttcttcgagc gtgagcaggg cgggttcgtc gccggcgggc cgggagaagt tgcagacgtt
     7981 ggtggcgatc ggccgcaccg acttgccgtc gcgcacccag ctgccgcgca tccggccgct
     8041 ccaggcgccg ccccgcttgc cgggacgcgg gtggtagtcg gtgtagaaga tcgagaggtg
     8101 cgtcccgtcg gcgtccttca cctcccacgc cttgacctcc gggtgataga cggggaggtc
     8161 gggccgaggc gtgaaggtga tgccgtacag gcggttcgcc acgtagaagg cgccctgctg
     8221 cacctgctcg agctggaagt aggggcgcag ctgctgctcg tcgaggtcgt acttcgcctt
     8281 ccgcaccttc tccgtgtagt accgccagtc gcccggcgcg agggtgaagt cgccgccctc
     8341 ggccttgatc tgctcctgga ggaccgccgc ctccttcaac gccatctcgc gggctggcgc
     8401 ccacaactgg ttgagcaggc cgtagacccc ggcgggagtc ttcgccatgt tctcctcgag
     8461 cacgaagtcg gcgtggctct tgtacccgag gaggttggcg cgctcgacgc ggagggcggc
     8521 gatgcgcgag acgatctgct tgttgtcgta ctggtcgccg tggtcgcccc gcgtcaggta
     8581 cgcgtcgagc agccgcctgc gcagctcgcg gttgtcggcg tactgcagga agggccagat
     8641 gctgggggcg tgcagcgtgt acacccactt gccaggcagg ccggcggcct tggcggcatc
     8701 cgcccccatc gccaccaccg aggcgggcag ccctgcgagg tcttccttgc gatcgatgac
     8761 cagcttgaag tcgttggtgt cgtgcagcag gttgtcgccg aacctcacgc tcagcatcga
     8821 cagctcggcg ttgatcttcc tgagctggtc cttcttcgct gcgtcgaggc tggcgcccga
     8881 ccggacgtac cccttgtagg tgtcctcgac cagcttcttc tggatcgcgg tcaggccgct
     8941 cgactcgcgg gcctcgtaca ccgctttgac gcgggcgaag agcttctcgt tcaaccgaat
     9001 ctcgtcgcgc agcgccgaga gcatcggcgt gacctcgcgg ttgatggcct ggagccgcgg
     9061 gttcgtctcg gccccctgca gcgccccgaa cacgccgctg acgcgcccga gcagcactcc
     9121 ggcgcgctcg agcgcctcca ccgtattggc aaaggtcggt gcctgcgggt ttcccgcaat
     9181 cgcctcgatc tccttccggt tctcggcgat ggccaccttg aaggccggga ggaactgctc
     9241 cgcctggatc tcctggaaag gcgggacctc gaacggggtc ttccactcct tgagcagcgg
     9301 gttgccggcg gccgcctcct gggcccggcc ggctgcggca gtcatgatga ggagcaggac
     9361 gacgaacagc ggtaagaagc gtctggtcat ggaataacct ttgagagaac cgaggacgcg
     9421 cggggcgacg cgcgggaaaa ccgtcgggat tctccgccac gggcgcgatg gggtcaagca
     9481 gggcggaaac agcgcggcgg gaaccgctgg gccgcccggt gcgttacttc cacaggtgcc
     9541 cccgcccccg gaccagtcca ttgagcggct tctggcacga ccttcgtcac ggcgtacgac
     9601 tcttcgcccg ggcgccaggc ttcaccgtga ccacggtgct ggtgctggcc ctcggcatcg
     9661 gagtcaacac cgccgtcttc accgccatca acggcctgtt gttcgagccg ctcggcggac
     9721 cggccgccgg cgagctggcc gcaatcttcg cccgcgacgc aaggcgcgac gggacctatc
     9781 gtcccttgtc gccagcggac tatcgtgagc tggcggccgc ctcgcgcccg tccggcgtgt
     9841 tcacttcgct catggcccac cagatgctga cggtgggcgt gaccgaggcc gggcgcaccc
     9901 gcaaggccta cgcgtgcctg gcctcgtcca cctatttcca gacactcggg atcgcgcccc
     9961 cggtcggccg cgcgttcctg cccgccgagg acgacccctc cgccgcgccc gtggcggtgg
    10021 tcagccgcgc gtactgggag cggaacggaa gcgaagcggc gctcgggcgc cggatcacca
    10081 tcaacaacca cgcgttcacg atcgtcggtg tcgtgcccga ggggttcagc ggcacgacgg
    10141 cactcctgtc gccggagtta tggcttccgc tgggggctgc cccgctgctt cgcgcgcacg
    10201 aacggcacga cgcgagcggc gaccctgaca ggatgcccgc gctgatggca gttgcacgcc
    10261 tgcgccccga gttgtcgctc gaggcggccg gcccgctcgt cgcctctttc gcccccagcc
    10321 tccggcaggc ggcgcccgtc gagaacgccg acctggagct gagcgtgggt ccgctgtcgc
    10381 ggttctccat gggagcggga ccacgcgacg acaccgaggc agccggcgtg ctcttcttcc
    10441 tgatgggcat ggcctcggtg gtgctgctca tcgcctgcct gaacctggcg aacctgctgc
    10501 tggccagggg gagcgcacgg tcgcgggaga tcgccgtccg cctcgcggtc ggcggcagcc
    10561 gcggccgcgt ggtgcggcag ctgctcgccg agtcactgct cctgtcgctg gcgggcgccg
    10621 gggtcggcgt ggccgtctcg gccggcggca cggcactgct gttccggacg ctctcggccg
    10681 tgcttccgct cccggtggtc ttcgatgcga cgcccgatcc ccgcacgctt gcggccgcgg
    10741 cagggcttgc cacgctcgcg acgctggcct ttggtctcgg tccggcaatg aacctcgcgc
    10801 gccgtgatct cctgcccgcg ctgagggaac ggcagagtca ccagccggcg ggacggtttg
    10861 gcttgcgggc ccgcaacctg cttgcggtcg gccagctcgc cctgtgcctc gccctgctaa
    10921 ccgcggctgg cctgttcgtc aagggggcgt gggaggccgg ggacgccgat cccggcttcc
    10981 ggctcgaccg gggcatcgtg ggctcgtccg acgcgtcgct ggccggctac gacgaactgc
    11041 gcgggcgcga ggcctaccgc cgagtggtgg cgcgcctgcg ccgcctgccc gggatcgagt
    11101 ccgccagcct ggcctctgcc gtgccgctcg gcctcgaggg gtcgtaccgg gaagtgaagc
    11161 ccgttggcgg aaccggctcg agcgaagccg cctgggccag cacggtggtc gtcggcgccg
    11221 ggtacttcgg cacggtcggc ttgccgctgc tgcgtggccg ggatttcacg ccggccgagg
    11281 aggagtcggc gtcgccgctg cggccggttc tcgtggacct gccgatggcc cggcgtctct
    11341 ggcccgacct ggacccgctg ggccggcaca tccagctgga ggtggagggc ggaggcggca
    11401 ccgcgtggag cgagccgttc gaggtcgtcg gcgtggtgcc cggggttcgc gacagcctgt
    11461 tcgacaagga gccggcggcc cacgtctacc tgccgtttgg agcggagtac cgcgccgcga
    11521 tgtacgtgca cgcccggctg gcgaccagcg accccgaggc aaccgaggca atcctctcgg
    11581 cgatgcggcg ag
//
LOCUS       DAOPQX010000086        11394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6535_length_11394_cov_4.465120, whole genome shotgun sequence.
ACCESSION   DAOPQX010000086 DAOPQX010000000
VERSION     DAOPQX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6535_length_11394_cov_4.465120"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PKK95_06595"
     CDS             complement(<1..722)
                     /locus_tag="PKK95_06595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC17915.1"
                     /translation="MSLARGTRLGPYEVLDQLGAGGMGAVYKARDTRLDRTVAVKVLL
                     PDAAADAAHRARFEREARAISRLSHPHICALFDVGRDIPSPAPPEGGVGSPVDFLVME
                     HLEGETLAERLELPHGRTLPLRETLRIGAELAEALSAAHRAGIVHRDLKPGNVMLTRT
                     GAKVLDFGVARLQAASLAAAGESSMSTCAVEPLTGTDMRLGTIPYMSPEQLDGKEADA
                     RSDLFALGVILYEAATGRRPFHG"
     gene            complement(901..1827)
                     /gene="rbsK"
                     /locus_tag="PKK95_06600"
     CDS             complement(901..1827)
                     /gene="rbsK"
                     /locus_tag="PKK95_06600"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF008353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOC17916.1"
                     /translation="MTPSIVVVGSSNTDMIIRLERIPRPGETILGGEFVTAPGGKGAN
                     QAVAAARAGGRVAFAARVGRDMFGDRALAGFVKDRIDVSLVVRDGSAASGVALIFVAK
                     DGENSIAVASGANANLAPADVKRASAAIAQADALVMQLETPLATVHAAAGIAAKAGVP
                     VILNPAPAPAKPLPARLLRQVSILTPNETEAELLTGIKVSDRVSASRAAAKLRALGVG
                     AVIITLGRRGAFVAAEAGEHLVPGFKVKAVDTTAAGDVFNGALAVALGEGRALADAVR
                     FANAAAAISVTRLGAQPSAPRRREIDRLLGKR"
     gene            complement(1871..2881)
                     /locus_tag="PKK95_06605"
     CDS             complement(1871..2881)
                     /locus_tag="PKK95_06605"
                     /inference="COORDINATES: protein motif:HMM:NF018828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17917.1"
                     /translation="MLIVESYILAVLMCGVTMLCWGSWANTQKLATREWRFQLFYWDY
                     AVGVLLLALLLAFTIGSVGGGGRSFLADLRQADAAWLGSAFVGGVVFNLSNILLVAAI
                     DIAGLAVAFPVGVGLALVLGVITTYIARPEGNVPMLAAGVACVLIAIVLDALAYRRLA
                     AAGRRTPVAGIVISVAAGLLMGWFYSFVAQAMGAIDPATRGLEAGKLSPYTAVVLFSA
                     GVLASNFIWNSLMMVRPFSGPPVPFGDYFTKGNARLHAIGILGGAIWSLGMSFSIIAS
                     TAAGPALSYGLGQGATMVGAMWGVFVWKEFKGAPEGTGKLLAAMFLFYAVGLGILVAS
                     KL"
     gene            complement(2905..4044)
                     /locus_tag="PKK95_06610"
     CDS             complement(2905..4044)
                     /locus_tag="PKK95_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOC17918.1"
                     /translation="MRFLSQLAAVGLTAASLCIVLLAWPVTVRPDAAQAGSPQAAARG
                     PAGTPQAPPRAAAASEPVKVILDVDLAEDVDDAGAVAVLHALANRGEAEILGMLVSSR
                     NESVVPALDAINTWYGRPDIPIGYQRGHVYGYRNTKDPDRETTSLYAEPITQAFPHDV
                     KRSSDAPAAASLCRKLLAAQPDASVTLVTTGFLTNLRELLDSRPDEASPLDGEALVKQ
                     KVIQWVCMGGVFPGGKFPDGGGEYNLMWDTSASVRAINDWPTPVVFSGFEIGNRVKVG
                     SRLRGTPEANPVRAAYQHYNQLHDREAWDQTAVLYAVRGARDYWTLSEPGLCLMHARV
                     THGYNEWVPSPRMRHRYLVEAMPPAALGKVIEDLMIEPPQERAGR"
     gene            complement(4059..5297)
                     /locus_tag="PKK95_06615"
     CDS             complement(4059..5297)
                     /locus_tag="PKK95_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17919.1"
                     /translation="MENRREFLKTLAMAAVVSGRAPALGANDRVRLAVIGTGNRGRIL
                     ARYFAALPECDLVAACDVRRSRLDETLADLGAKVEACGDYRRILDRNDIDAVVVATPD
                     HWHAPMIVEACAAGKDVYVEKPLTHTVEDAVRAVEAAKTHRRVVQLGVQQRSGQHYQE
                     AVALVRDGLLGKVTHAVLIQPGGYSQVVAPTEPPPADLDWEMFQGPAPRHPFTPSRLR
                     WRGFYDYGGGLITDWGVHLVETALWALGADAKTPLQTAASAQYVAFPRDLEQLPDAFS
                     CSWQFDDFVMSFTNVVPPTVDFGQQGTYLYGPKGVLHTNRSGYRVLPYPARKDRDGKV
                     IEPAIDGRLISHREDYDKNDVYTIAHARNFLECVRSRKAPVADITTVGFHVTLPCLLA
                     RQAVKEGKALGWNGKKAVPL"
     gene            complement(5310..5684)
                     /locus_tag="PKK95_06620"
     CDS             complement(5310..5684)
                     /locus_tag="PKK95_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17920.1"
                     /translation="MRRLVVLVALAALTAVPAFAQDAAREGTVTGTWEVSVESPQGPM
                     TITATYKQEGEKLTGMHVSEMGEAPLEGTVKGSEFTYVLTIQGPDGPLALKHTGKVDG
                     DTITGTVELFGDTIPWTAKRKK"
     gene            complement(5832..6569)
                     /locus_tag="PKK95_06625"
     CDS             complement(5832..6569)
                     /locus_tag="PKK95_06625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC17921.1"
                     /translation="MEFLELMAISHRYMELLNPSTPEKVLKLGKMLGMKEGARVIDYG
                     SGCGEPLALWCKEYGITGVGVDLSPDFCERAVKKLSGQGLSDRIQIVCGKGADYELPG
                     QPFDVATCIGATFVFGGYRETVQALKKAVHSKARLGIGDVYWASDTVPPGYAQREVSA
                     RTETELLRVTREEGFEIEYVIRASRDDWDRYYSDNWHGFLRWLEENPSHPDRQQVLEH
                     FHQWQDDYSRYERPYMGWAMYVLKPGG"
     gene            complement(6595..7368)
                     /locus_tag="PKK95_06630"
     CDS             complement(6595..7368)
                     /locus_tag="PKK95_06630"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC17922.1"
                     /translation="MSKSRVRILAGIAILVLATVPILAQRVAVLPQSNDEAVAKVPVG
                     FTPIFNGKDLTGWHVSKTNHHGTTPSYQVIHGILVGTQQPFGQGGVLLTDKKYGEVEV
                     YLEVKPDWGCDSGLFFWADEDGGGYQVLLDYLPGGTMVAGMYGERLKDVNFNGAGWDA
                     MTPAERQELQAKRNEAWQKVWKRDEWNTVRARIEGNPPRVTTWLNDVMMFQGRDTANH
                     ATGGATSGSIAIQVHGGERWRPGAFWRWRVIAVKELGAE"
     gene            complement(7438..7914)
                     /locus_tag="PKK95_06635"
     CDS             complement(7438..7914)
                     /locus_tag="PKK95_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17923.1"
                     /translation="MNLPSILLWGFAATVTMTTVMAASQGLGLSRMSIPFILGTMVTP
                     HRDRAMLLGSLLHVLNGWIFALVYAAAFESWGGAGWWRGAAIALVHAGFVLTAGMAML
                     PGLHPRMKSEQAGGASAARLQPPGFLALNYGERTPLVTLAAHIVYGALLGAFYKMQ"
     gene            complement(7911..9767)
                     /locus_tag="PKK95_06640"
     CDS             complement(7911..9767)
                     /locus_tag="PKK95_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOC17924.1"
                     /translation="MASYRPIGDYAVIGNCRTAALVASDGSIDWLCLPRFDSPSVFAA
                     ILDQTGGGRFALRPAGEIRAVSRRYLPGTNVLETTFTTAAGRARLTDAMTPSLEAAEA
                     ALRPSHEIVRRIEVIEGEVEVEVLFDPRPGYGQRAARIQRAGASAVLCTWGSEAIALR
                     SEVPLGAGGSSVLRGCERLRAGDRRFVVLGYDGHVPSVIPAVGGDAERTIALAGRWWQ
                     DWSRGFTYEGPYREAVLRSALVLKLMTFAPSGAIVAAPTTSLPEAIGGDRNWDYRYCW
                     LRDASLTLRALFDVGFTAEAEAFNAWLLHATRLTWPRLQILYDVYGRTRLEERELGHL
                     EGYEGSRPVRAGNGAAGQLQLDVYGEVVEAAYRYVSRGGGLDRTSGKMLAGLGRTVAR
                     SWRAPDEGIWETRAGRRHHTFSRVMCWVALDRLIRLGEAGYLRAGLTEFRRERDAIRA
                     EVEARGYNESLGSYVSELDGDEVDAALLLVGLHGYSPVEGERMRGTLARVRERLGTNG
                     LLFRNRDEDGLRGREGAFGICSFWAVEALARSGEADEALDTFERLLAFANDVGLYAEE
                     IDPVTGAALGNFPQAFTHVGLINAALTLAGHSESPMGCAGAAAPQAAAERET"
     gene            10063..11079
                     /locus_tag="PKK95_06645"
     CDS             10063..11079
                     /locus_tag="PKK95_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC17925.1"
                     /translation="MRKLVLRNFQSPGDIVMLTAAVRDLHRAHPGEFLTDVRTSCPAL
                     WDYNPHLTPLSEDDPDVEVVDCHYPLIHQSNTTPCHFLHGFPAYLNETLGLRIRVTEF
                     RGDIWIGEEERQWFDRVLSQGGLDLPFWLLVSGGKYDYTIKWWDHARYQRVIDHFRGR
                     IQFVQVGEASHYHPPLRGAVDLRGQTDLRELVRLVYHSQGVLSPVSLLMHLAAAVETP
                     PGQPENRPCVVVAGGREPPHFTAYPHHQFIHTVGALRCCSNGGCWKSRTLPLGDGDEK
                     DAPEQLCVDVVGSLPRCMDMITADEVIRRIEMYFDGGILASRHPAASSSVEEEEEEVD
                     EVTQ"
     gene            11167..>11394
                     /locus_tag="PKK95_06650"
     CDS             11167..>11394
                     /locus_tag="PKK95_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17926.1"
                     /translation="MRRRGRHFPRGLRGREPSQPHPPEFQRDLEFLVGRIELERVEQL
                     VELQLQFEQFEQLGQFEQFFQQLEPVEQFEQL"
BASE COUNT         1703 a         4069 c         3890 g         1732 t
ORIGIN      
        1 ccgtggaacg gccggcgccc ggtggccgcc tcgtagagga tcaccccgag cgcgaacagg
       61 tcgctgcggg cgtcggcctc cttcccatcg agttgctcgg gcgacatgta gggaatcgtc
      121 ccgaggcgca tgtccgtgcc ggtgagcggt tccaccgcgc aggtcgacat cgacgattcg
      181 ccagcggccg cgagcgaggc ggcctggagc cgggcgacgc cgaagtcgag caccttggcg
      241 cccgtccggg tcagcatcac gttgcccggc ttcaaatctc tgtggacgat gcccgcccgg
      301 tgagcggcgg acagcgcctc ggccagctcg gcgccgatgc gcagcgtctc gcggagcggc
      361 agcgtccgtc cgtgcggcag ctcgagacgc tctgcgagcg tctcgccctc gaggtgctcc
      421 atcacgagga agtccaccgg gctgccgacc ccgccctcag gcggcgcggg cgacgggatg
      481 tcgcgcccca cgtcgaacag tgcgcagatg tgcgggtggc tgaggcgcga gatggctcga
      541 gcctcgcgct cgaaccgtgc gcggtgcgcc gcgtcggcgg ccgcgtccgg cagcaggacc
      601 ttgacggcga cggtgcggtc gagtcgcgtg tcgcgcgctt tgtacaccgc gcccattccg
      661 ccggccccca gctggtcgag gacctcgtag gggccgagcc tggtaccgcg ggcgaggctc
      721 acacttcggc tgctttccac cgcttccgac ctttgagcgg cctataccca cggtacagac
      781 tacgcgccgg ccgggcggcg aacgatgatc tgggtcacag cgaagagaag gcgagcggcg
      841 tgccagcccg gctgttcaag gccggcacgg ctcgccgctg gcgggcgcag aacgcgcgcc
      901 tcaccgtttt ccgagcagcc gatcgatctc ccggcgccgc ggcgcagacg gttgcgcgcc
      961 gagcctcgtc accgagatgg ccgcggcggc gttggcgaag cggacggcgt ccgcaagcgc
     1021 gcgcccctcg ccgagagcga cggcaagcgc gccgttgaag acgtcgcccg cagcggtcgt
     1081 gtccaccgcc ttcaccttga agccgggcac caggtgctcg cccgcctcgg cggcgacgaa
     1141 ggcgccgcgc cgcccgagcg tgatgatgac ggcccccacc cccagcgcgc gcaacttggc
     1201 ggcagccctc gacgccgaca cccggtcgga caccttgatg ccggtcagca actcggcctc
     1261 cgtctcgttg ggcgtgagga tgctcacctg gcgcagcagg cgggcgggga gcggtttggc
     1321 cggcgcgggg gcggggttga ggatgacggg gacgcctgcc tttgcggcga tgccagctgc
     1381 ggcgtgcacg gtggcgagcg gcgtctcgag ctgcatgacc agcgcgtcgg cctgggcgat
     1441 ggccgcagag gcacgcttca cgtccgccgg cgcgaggttg gcgttcgcgc ccgaggcgac
     1501 cgcgatggag ttctctccgt ccttcgccac gaagatcagc gcgacccccg acgccgcgct
     1561 gccgtcgcga acgacgagcg agacgtcgat ccggtccttg acgaagccgg cgagcgcccg
     1621 gtcgccgaac atgtcacggc cgacgcgcgc ggcgaaggcg acccggcccc cggcccgggc
     1681 cgcggccacc gcctggttgg ctcccttgcc tcccggcgcc gtcacgaact cgccgcccag
     1741 gatcgtctcg cccggccgcg ggatccgctc gaggcggatg atcatgtcgg tgttggagct
     1801 gccgacgacg acgatggaag gagtcataag gcaggctcaa ggctcagggc gcacggctcc
     1861 ggcaggttga tcacagtttc gacgccacca ggattcccag cccgactgcg tagaacagga
     1921 acatcgcggc gagcagcttc cccgtgccct ccggcgctcc cttgaactcc ttccacacga
     1981 acacccccca catcgccccc accatcgtcg cgccctggcc gaggccgtac gagagcgcgg
     2041 gccccgcggc cgtcgaggcg atgatgctga agctcatgcc gaggctccag atggcgccgc
     2101 cgaggatgcc gatcgcgtgc agccgcgcgt tgcccttcgt gaagtagtcg ccgaacggca
     2161 ccggcgggcc cgagaacggg cgcaccatca tcaggctgtt ccagatgaag ttcgacgcca
     2221 gcacgccagc gctgaagagg acgacggccg tgtagggcga cagcttgcca gcctcgaggc
     2281 cgcgtgtcgc ggggtcgatg gcccccatcg cctgtgcgac gaagctgtag aaccatccca
     2341 tcagcagccc cgccgcgacc gagatgacga tccccgcgac cggcgtcctg cggccggcgg
     2401 cggcgaggcg gcggtaggcg agggcgtcga ggacgatggc gatcaggacg caggcgacgc
     2461 cggcggccag catcgggacg ttgccctcgg ggcgggcgat gtaggtggtg atgaccccga
     2521 ggacgagcgc gaggcccacg cccacaggga aggcgaccgc gagccccgca atgtcgatgg
     2581 ccgcgacgag caggatgttc gacaggttga agacgacgcc gccgacgaac gccgacccga
     2641 gccaggccgc gtcggcctgc cgcaggtcgg ccaggaagct gcgtccgccg ccgccaacgc
     2701 tgccgatcgt gaacgcgagc agcagcgcaa gcagcaggac gccgacggcg tagtcccagt
     2761 agaacagctg gaagcgccac tcgcgcgtcg ccagcttctg ggtgttcgcc cacgaccccc
     2821 agcagagcat ggtgacgccg cacatcagca ccgcgaggat gtacgactcg acgatcagca
     2881 tcgaccggct ccctgatccc tgatctacct tcccgccctt tcctgtggcg gctcgatcat
     2941 caggtcctcg atcacctttc caagcgcggc gggcggcatc gcctcgacca ggtagcggtg
     3001 gcgcatcctg ggcgacggca cccactcgtt gtagccatgg gtgacgcgcg cgtgcatcag
     3061 gcagaggccc ggctccgaga gggtccagta gtcgcgcgcg ccgcgcaccg cgtagagcac
     3121 ggctgtctgg tcccaggcct cgcggtcgtg cagctggttg tagtgctggt aggccgcgcg
     3181 caccgggttg gcctcgggcg tgccgcgcag ccgcgacccc accttcacgc ggttgccgat
     3241 ctcgaagccg ctgaagacga ccggcgtggg ccagtcgttg atcgcgcgca ccgacgccga
     3301 ggtgtcccac atcaggttgt actcgccacc cccatccggg aacttcccgc ccgggaagac
     3361 cccgcccatg cagacccact gtatgacctt ctgcttcacc agcgcctcgc cgtcgagcgg
     3421 gctcgcctcg tcggggcgcg agtcgagcag ctctcgcagg ttggtcagga agccggtggt
     3481 gacgagggtg acgctcgcgt cgggctgggc ggcgagcagc tttcggcaga gggaggccgc
     3541 ggcgggggcg tcgctgctgc gcttcacgtc gtgggggaac gcctgcgtga tcggctcggc
     3601 gtagagcgag gtcgtctcgc ggtcgggatc cttcgtgttc cggtagccgt acacgtgccc
     3661 ccgctggtag ccgatcggga tgtcgggccg tccgtaccag gtgttgatgg cgtcgagcgc
     3721 gggcacgacc gactcgttcc tggacgagac cagcatgccg aggatctcgg cctcgccccg
     3781 gttggccagc gcgtgcagga cggccaccgc ccccgcatca tctacgtcct cggcgaggtc
     3841 gacgtcgagg atgaccttca ctggctcgga ggcggctgcg gcgcgggggg gcgcctgcgg
     3901 ggtgccggca ggcccccggg cggccgcctg gggcgaacct gcctgcgccg catcgggccg
     3961 gacagtcacc ggccaggcga ggagcacgat gcacagggaa gccgcggtca gtcccaccgc
     4021 ggcaagctga ctcaggaagc gcatagcctc ttgccccctt acagcggcac cgctttcttt
     4081 ccgttccacc ccagcgcctt cccctccttc accgcctggc gcgcgaggag gcaggggagc
     4141 gtgacgtgga agccgaccgt cgtgatatcg gcgaccggcg ccttccgcga ccgcacgcac
     4201 tcgaggaagt tgcgcgcgtg cgcgatggtg tagacgtcgt tcttgtcgta gtcctcgcgg
     4261 tggctaatca gccggccgtc gatggccggt tcgatgacct tgccgtcgcg gtccttcctc
     4321 gccgggtagg gcagcacgcg gtagcccgac cggttcgtgt gcagcacccc cttcggcccg
     4381 tagaggtacg ttccctgctg gccgaagtca accgtcggcg gcaccacgtt ggtgaacgac
     4441 atgacgaagt cgtcgaactg ccacgagcac gagaaggcgt cgggcagctg ctcgaggtct
     4501 ctcgggaacg cgacgtactg cgccgacgcg gcggtctgca gcggcgtctt cgcgtccgcc
     4561 cccagcgccc agagcgcggt ttcgaccagg tggacgcccc agtcggtgat gaggccgccg
     4621 ccgtagtcgt agaacccgcg ccaccggagg cggctcgggg tgaacgggtg ccgcggcgcc
     4681 ggcccctgga acatttccca gtcgaggtct gccgggggcg gctcggtggg cgcgaccacc
     4741 tgcgagtagc ctcccggctg gatcagcacg gcgtgggtga ccttgccgag cagcccgtca
     4801 cggacgagcg ccaccgcctc ctggtagtgc tggccgctgc gctgctgtac cccgagctgc
     4861 acgacgcggc ggtgcgtctt cgccgcctcg accgcgcgca ccgcgtcttc caccgtgtgc
     4921 gtgagcggct tctcgacgta gacgtccttt cctgcggcgc acgcctccac gatcatcggc
     4981 gcgtgccagt gatcgggggt ggcaacgacg acggcatcga tgtcgttgcg gtcgaggatg
     5041 cgcctgtagt cgccgcaggc ttccaccttc gcgcccaggt cggccagcgt ctcatcgagc
     5101 cgtgaccgcc ggacgtcgca ggcggcgacc aggtcgcact cgggcagcgc ggcgaagtag
     5161 cgggccagga tgcggccgcg gttgccggtg ccgatgaccg cgaggcggac ccggtcgttg
     5221 gccccgaggg cgggggcgcg gccggacacg acggccgcca tcgccagcgt cttcaagaac
     5281 tcgcgccggt tctccatgcg tccccctcgt tacttcttgc gcttcgccgt ccagggaatc
     5341 gtgtcgccga acaattcgac ggtgccggtg atcgtgtccc cgtccacctt tccagtgtgc
     5401 ttcagggcga gcgggccatc ggggccctgg atggtgagga cgtaggtgaa ctcgctgccc
     5461 ttcaccgtcc cctcgagcgg agcctcgccc atctcgctca cgtgcatgcc ggtcagcttc
     5521 tcgccttcct gcttgtaggt ggcggtgatc gtcatcgggc cctgcggcga ctcgaccgag
     5581 acctcccacg tccctgtcac agtcccctcc cgggcggcgt cctgagcgaa cgccggcacc
     5641 gccgtcaacg ccgcgagcgc cacgagcacg acgagcctac gcatgatggc ctcctcacct
     5701 gttgggagcg acccttgtgg tcgcccgtga ccgcctcgta ggggcgaccc ttgtggccct
     5761 tcgaccgagc tcagggccac cctgagcctg ccgaaggctg gtcgcccccg aactacttcc
     5821 cacggcgttg cttatccacc cggcttcaaa acgtacatcg cccagcccat gtaaggtctc
     5881 tcgtaccgcg aatagtcatc ctgccactgg tggaagtgtt ccaggacctg ctgacgatcg
     5941 ggatgggagg gattttcttc cagccagcgg aggaagccat gccaattgtc ggagtagtac
     6001 ctgtcccagt catcacggct ggccctgatg acgtattcga tctcgaagcc ttcctcgcgg
     6061 gtcactcgca gaagctcggt ctcggtacgg gcgctcacct ctctctgggc gtagccaggc
     6121 gggaccgtgt cgctcgccca gtagacgtcc ccaatcccca accgcgcttt cgaatgaacg
     6181 gccttcttca gcgcctgcac ggtttcccgg tagccgccga agacgaaggt ggccccgatg
     6241 caggtggcga cgtcgaaggg ttgccccggc agctcgtagt cggcgccctt gccgcacacg
     6301 atctggatgc gatcggacag gccctggccg gacagcttct tcaccgcccg ctcacagaag
     6361 tccggcgaga ggtcgacgcc gaccccagtg atgccgtact ccttgcacca gagcgccaga
     6421 ggctcaccgc aacccgagcc gtaatcgatc acacgggcgc cctctttcat gccaagcatc
     6481 ttcccgagct tgagcacctt ctcaggggtg gaggggttga gcagctccat gtagcggtgt
     6541 gaaatggcca tcagctcgag aaattccatt gccgtcgatc tcccctcccg atcgctactc
     6601 cgcccccagc tccttcaccg cgatcaccct ccaccgccag aacgcgcccg ggcgccaccg
     6661 ctcgccgccg tgcacctgga tggcgatcga ccccgaggtc gcgccgccgg tggcgtggtt
     6721 ggccgtgtcg cgtccctgga acatcatcac gtcgttcagc cacgtggtga cccgcggcgg
     6781 gttcccctcg atgcgcgcgc gcacggtgtt ccactcgtcg cgcttccaca ccttctgcca
     6841 cgcctcgttg cgcttggcct gcagctcctg gcgctcggcc ggcgtcatcg cgtcccaccc
     6901 ggcgccgttg aagttcacgt ccttcagccg ctccccgtac atccccgcca ccatcgtccc
     6961 gccgggcagg tagtcgagca gcacctggta gccgccgccg tcctcgtcgg cccagaagaa
     7021 gaggccgctg tcgcagcccc agtcgggctt cacctcgagg tacacctcca cctcgccgta
     7081 cttcttgtcg gtcagcagga cgccgccctg cccgaacggc tgctgcgtgc cgacgaggat
     7141 gccgtggatc acctggtagg aaggggtggt gccgtggtga ttggtcttgc tgacgtgcca
     7201 gccggtgagg tcctttccgt tgaagattgg cgtgaagccg accgggacct tcgcgacggc
     7261 ctcgtcgttc gactggggga ggacggcgac gcgctgggcg aggatgggca ccgtcgcgag
     7321 caccaggatg gcgatgccgg cgaggatgcg cacgcggctc ttgctcatgg tctcctcgat
     7381 gggggaatgg cccctgcggg cgtgttggga gcggattgta tcagcacatc gccgccgcta
     7441 ctgcatcttg tagaacgctc cgagcagcgc gccgtagacg atgtgggcgg cgagcgtcac
     7501 caggggggtg cgctcgccgt agttgagggc gaggaagccg ggaggctgca gccgtgccgc
     7561 ggaggcgccg cccgcctgct cgctcttcat gcggggatgg agccccggca gcatggccat
     7621 gcccgcggtc aggacgaagc cggcgtgaac cagcgcgatc gctgcaccac gccaccaacc
     7681 ggctcctccc cagctctcga acgccgccgc gtacaccagc gcgaagatcc agccgttgag
     7741 gacgtggagc agcgagccga ggagcatcgc ccgatcgcgg tgcggcgtga ccatcgtccc
     7801 caggatgaag gggatgctca tgcggctcag gccgagtccc tggctggcag ccatcacggt
     7861 ggtcatggtg acggtcgccg cgaagcccca gagcaggatc gacgggaggt tcatgtctcg
     7921 cgctccgcag ctgcctgtgg tgctgccgca ccagcacatc ccatggggga ttcgctgtgg
     7981 ccagcgagcg tcagcgcggc gttgatgagg ccgacgtgcg tgaaggcctg agggaagttg
     8041 ccgagggcgg cgccggtgac cgggtcgatc tcctcggcgt acagcccgac gtcgttcgcg
     8101 aaagcgagca gccgctcgaa ggtgtcgagc gcctcgtctg cctcccccga gcgggcgagg
     8161 gcctccaccg cccagaagct gcagatgccg aaggcgccct cgcggccgcg cagcccgtcc
     8221 tcgtcgcggt tccggaacag cagcccgttc gtgccgaggc gctcgcgcac gcgcgccagc
     8281 gtgcctcgca tgcgctcccc ctcgacgggg gagtagccgt ggaggccgac tagcagcagg
     8341 gccgcgtcca cctcgtcgcc atccagctcg ctcacgtagc ttcccagact ctcgttgtag
     8401 ccgcgcgcct ccacctcggc tcgaatggca tcgcgctcgc ggcggaactc ggtgaggccg
     8461 gcgcgcaggt aacctgcctc gccgaggcgg atcagccggt cgagggccac ccagcacatg
     8521 accctcgaga acgtgtggtg ccgccgcccc gctcgcgtct cccagatgcc ctcgtctggc
     8581 gcgcgccacg agcgcgccac cgtccggccg aggccggcca gcatcttccc gctcgtccgg
     8641 tccagcccgc cgccccgcga gacgtatcga tacgccgctt ccaccacctc gccgtacacg
     8701 tcgagctgca gctgcccggc ggcgccgttg cccgcccgga ccggcctgga gccctcgtag
     8761 ccttccaggt gaccaagctc ccgttcctcg agacgggtcc tgccgtacac gtcgtacagg
     8821 atctgaagcc gcggccacgt caggcgcgtg gcgtgcagga gccaggcgtt gaaggcctcg
     8881 gcctccgcgg tgaagcccac gtcgaagagg gcgcgcaggg tgagcgacgc gtcccgcagc
     8941 cagcagtacc ggtagtccca gttccggtcg cccccgatgg cctcgggcag cgaggtggtt
     9001 ggcgcggcca cgatcgcgcc ggacggcgca aaggtcatga gcttgagcac gagcgcgctg
     9061 cgaaggacgg cctcccggta cgggccctcg tacgtgaagc cgcgcgacca gtcctgccac
     9121 caccgcccgg ccagggcgat cgtccgttcg gcatcgccgc ccacggccgg gatcaccgac
     9181 ggcacgtgcc cgtcgtaccc caggaccacg aaccgccggt cgcccgcgcg gagacgctcg
     9241 cacccgcgaa ggacggagct gccgccggcc cccaggggca cctcgctcct gagggcgatg
     9301 gcctccgagc cccaggtgca gaggacggcc gacgcgcctg ctcgctggat tcgcgcggca
     9361 cgctgcccgt atcccggcct gggatcgaac aggacctcca cctcgacctc gccctcgatc
     9421 acctcgatcc gccggacgat ctcgtgcgag ggccggagtg cggcttcggc cgcctcgagc
     9481 gaaggggtca tcgcatccgt gaggcgcgcc ctgccggccg ccgtggtgaa ggtcgtctcc
     9541 aggacgttcg tgccggggag gtagcggcgg gagaccgcgc ggatctcgcc cgcgggccgc
     9601 agggcaaatc gcccgccccc cgtctggtcg aggatcgctg cgaagaccga gggactgtcg
     9661 aagcggggca ggcagagcca gtcgatggag ccgtcgctgg ccacgagggc ggccgtgcgg
     9721 cagttgccaa tcacggcgta gtcgccgatg gggcggtagg aggccattca atcatgggac
     9781 cacgggcggc gggcacaggt ggctgagcag gggcggccca ggcgtcccgc ggtcccagtg
     9841 gcccaggcgt cccgcggtcc cagtggcaca ggcgtcccgc ggtcccagtg gcacaggcgt
     9901 cccgcctgtg agtacgcgcg gcgaaaccgg ctttcctttc acgtaagctc gtcgttggcc
     9961 accgggccct ccgatcccct gattccgccc gaattcgccg atcttccgac cgcgcaacag
    10021 ccgcgctgcc cggcaccctt gttgcggaag agcgcaacag ccatgcgcaa gctcgtgctc
    10081 cgcaacttcc agtcgcccgg cgacatcgtg atgctgaccg ccgccgtccg cgacctccac
    10141 cgcgctcacc cgggtgagtt cctcaccgac gtgcggacct cctgccccgc gctgtgggac
    10201 tacaacccgc acctcacccc gctcagcgag gacgatcccg acgtggaggt cgtcgactgc
    10261 cactacccgc tgatccacca gtcgaacacc acgccgtgcc acttcctgca cggcttcccg
    10321 gcctacctga acgagacgct gggcctccgc atccgggtga ccgagttcag gggagatatc
    10381 tggatcggcg aggaagagcg ccagtggttc gaccgcgtgc tgtcacaggg cggcctcgat
    10441 ctgccgttct ggctgctggt ctcgggcggg aagtacgact acaccatcaa gtggtgggac
    10501 cacgctcgct accagcgggt catcgaccac ttccgcgggc gcatccagtt cgtgcaggtc
    10561 ggcgaggcgt cacactacca cccgcccctg cggggggcgg tggacctgcg cgggcagacc
    10621 gacctccgcg agctggttcg gctcgtgtac cacagccagg gtgtgctgag ccccgtcagc
    10681 ctgctgatgc acctcgccgc cgccgtcgaa acccctcccg ggcagcccga gaaccggccc
    10741 tgcgtggtcg tcgccggcgg cagagagccg ccccatttca ccgcgtaccc gcatcaccag
    10801 ttcattcaca ccgtcggcgc gctgcggtgc tgctccaacg gcggctgctg gaagtcgcgg
    10861 acgctgcccc tgggcgacgg cgacgagaag gacgcccccg aacagctgtg cgtggacgtc
    10921 gtcggtagcc tgcctcgctg catggacatg atcacggcag acgaggtgat ccgccggata
    10981 gagatgtatt tcgacggcgg gattctcgcg agtcgtcacc cggccgcgag ctcgtccgtc
    11041 gaagaagagg aggaggaggt cgatgaagtc acgcagtgac caggagtcgc gcgatggccg
    11101 ccgggcagcc gacaccgcgg aagacgagcg ccggacggcc gagcgccgga gcttcctgct
    11161 gctgctgtgc ggcggcgcgg caggcacttt cctcgcgggc tgcgggggag ggaacccagc
    11221 cagccccacc ctcccgagtt ccagcgggat ctcgagttcc tcgtcggccg catcgagctc
    11281 gagcgcgtcg agcagctcgt cgagctccag ctccagttcg agcagttcga gcagctcggg
    11341 cagttcgagc agttcttcca gcagctcgag ccggtcgagc agttcgagca gctc
//
LOCUS       DAOPQX010000087        11307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6598_length_11307_cov_5.133932, whole genome shotgun sequence.
ACCESSION   DAOPQX010000087 DAOPQX010000000
VERSION     DAOPQX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6598_length_11307_cov_5.133932"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKK95_06655"
     CDS             complement(<1..454)
                     /locus_tag="PKK95_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17927.1"
                     /translation="MAVHDDSTGYQAARTASAILDRAARGKLVVAGSDRRTYLHAMLT
                     CEVAPLQPGSGTYGAFLTPQGRMIADMRLLELGDLILMDLDRARAADVLQRLDQFVFS
                     EDVQLGDVSDTFGLISLVGPAASGVASRALGGGAAEGLTSSLLDSWAEF"
     gene            484..2151
                     /locus_tag="PKK95_06660"
     CDS             484..2151
                     /locus_tag="PKK95_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC17928.1"
                     /translation="MRKLHSTVVFALLALAPALLEAQPLAGGGVQFTVFLRGTPVGVE
                     ETTVARSADGLVITGTSRLGPPLEVIVRRAEIRYDASGNPLSCFVEGSLRQRQLVLST
                     TVEGNTATTDLTEGTSRSRQAHEIAPGAVLLSSVYFGGHEALAARLQGKKEGDEIPIY
                     VPGQGEATARVVSATDERIQTSSGMLKARRFQVALEQQGKTTDTEVWVDDQGRLVRFS
                     TASQTIDVVRTDVASVSARREAVARPGDESVRIPANGFTLAGTLSRPGQPAKGAKLPA
                     IVLVGSIGVTDRDESRAGIPILGQLASALADAGYLVIRYDHRGIGQSGGRAESAEIGD
                     YAEDVRSVVRFMRKRKDVDRDRLAVLGYDYGGSVAMTAAARDGDIKALVLVATPGLAG
                     GDLVLEQQRAALAKMDLTEAEKQRRIDLQQRINEAVVSGRGWDQIPPAMRQQAETDWF
                     RSLLAFDPAEQMRRVRQPVLVVHGGLDAELGPHHAEALASMAEARKNPAGEKVRLAVV
                     PGVNHLLVPSREGTVDEYASLGGASVSPEVTGAITTWLGEALAAAKK"
     gene            2160..2465
                     /locus_tag="PKK95_06665"
     CDS             2160..2465
                     /locus_tag="PKK95_06665"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC17929.1"
                     /translation="MWILRGNGADGQQTLTVRLMPGTARTLGRGPQANFIVDEALVSR
                     LHCRFVVDGAGKLAVEDLGSTNGTWVNDQRVERADLAPGDRVRAGQLELVVETSRGL"
     gene            complement(2472..3122)
                     /locus_tag="PKK95_06670"
     CDS             complement(2472..3122)
                     /locus_tag="PKK95_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC17930.1"
                     /translation="MIFETREAPPFYKNGFVLGCEETRQAVLIDPGDEVEQLLAVVDE
                     QRLEVRHILLTHAHVDHVAGLAAAKKQLDAAIWLHADDLPLYNAALEQARFFGFNIET
                     PPPVDRFYEIPGAIEFGRYRVEVLHTPGHTPGGVSLLVGPTGRPGKKLFSGDTLFAGS
                     IGRTDLPGGDHDTLIGSIRGVLFKLSDDVEVYPGHGPRTMIGQERMTNPFLTGERP"
     gene            complement(3186..4154)
                     /locus_tag="PKK95_06675"
     CDS             complement(3186..4154)
                     /locus_tag="PKK95_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOC17931.1"
                     /translation="MDVLEEARRARQRTAGRLRRTPLIRSDWLSERAGADVHLKLESL
                     QVTSSFKARGALNAVLALAERTRPGEPLPRVVTASAGNHGRALAWAASSIGLPVVIFT
                     PRSAPAAKLSAIRRYPSELRAQADDYEGAEALAKEHARRTGAFYLSPYSHPDLLAAIG
                     GIALEILEDLPEVSQVIVPVGGGGLIAGIAQVLKALRPAARVIGVEVEASHPFTASLA
                     AGRIVEVKVGPTLADGLAGNMDPDNIAFPIVQRLVDRVVLASEPALEAAVRGLVEAEH
                     LVAEGAGATAVAAVLAGTIPRCPGPAAVVVSGANIDSSRLASILAA"
     gene            complement(4085..5050)
                     /locus_tag="PKK95_06680"
     CDS             complement(4085..5050)
                     /locus_tag="PKK95_06680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC17932.1"
                     /translation="MEQTEKYTILDPISIQGLLGDLFRARDNVTGRTVALRVVREEVA
                     GDPVRRAALLADAGKAREVSHPHIAALFDVGEQDGFLYLAHEYVPGQTLAAMLDKGPL
                     DPEAALELALQIAEALARVHRHGLIYRALSATSVIVNPRDQAKLLDVGLAAWTSAGQV
                     RLGLSPQTAEGQERREEDYDRAASRPGAYVSPEEALGESAVTRSDLFSLGVLLFQLVT
                     GQMPFDGGSDSATAFKILHATPPAPSSLNPAVPPGFDAAVAGMLRKTVEMRPHDAGAV
                     IDQLRRLRHVAGAASAAPREESRWTFWRRLGGRGSGRRGDSGGRR"
     gene            5087..5809
                     /locus_tag="PKK95_06685"
     CDS             5087..5809
                     /locus_tag="PKK95_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOC17933.1"
                     /translation="MRAIFFDLVGTLVEPRGPVGAQYAAIARRHGEHADADALGAAFA
                     REMASTPAVQAWGLSREAAEAAERGWWHSLVRRVLDLAGAGGLLDAARFEPFFDDLYR
                     HFTTADAWRLYPDVLPALDRLETAGIATGLITNYDSRIYRLVESLGLASSLGSITIPA
                     SAGATKPARAIFGQALFCHRLAASEAGHVGDSLADDYQGALEAGLHAVLLDRDGHHAA
                     LAGVRRAGNLEEAVALLEKELG"
     gene            complement(5828..6499)
                     /gene="lipB"
                     /locus_tag="PKK95_06690"
     CDS             complement(5828..6499)
                     /gene="lipB"
                     /locus_tag="PKK95_06690"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOC17934.1"
                     /translation="MRRIGVRRLGLVPYGEALELQRALADDRRQGRVGDVLLLLEHPH
                     VITLGARSDAARSNVIATPERLRELGVELFETGRGGDVTYHGPGQLVAYPIFDLRPDR
                     QDVHRYVRDLEEVMIRVTSAFGVEAGRIGGLTGAWVVADKIGAIGVRISRWITSHGFA
                     YNVNTRLEYFNLIVPCGIRDHGVTSLSKLLGRELPMGEVEDAVVRAFGEVFEAEIFEA
                     EIAQG"
     gene            complement(6496..7815)
                     /locus_tag="PKK95_06695"
     CDS             complement(6496..7815)
                     /locus_tag="PKK95_06695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOC17935.1"
                     /translation="MPTNVLMPQMGESIAEGTIVRWLKRVGDKVDRDEPLFEISTDKV
                     DAEIPSPTAGVVTEIRSKEGETVPVNQVVAVIDEAPGKGAAAAPPPAEGAAAPSQPPP
                     PPPSAIQPAQPAPAAPPPPPPAVPVDAAAEGPAADELRRQRSSPLVRKIAAEHGVDIS
                     QVQGSGIGGRVTKQDILDYLESPKPAAPPAAPAPAPPAPPASPGPAVKAAEGDEVVPM
                     TPMRRRIAEHMVLSKRTSAHVHSVFEVDFGNVEKLRQAKKAEYERAGAKLTYLSFIVK
                     AVVDGLRAVPVVNASIDGENVIYRKDVNVGIAVALDWGLIVPVIKHADEKNILGLSKA
                     IQDLASRARAKQLKPEEVQGGTFTITNPGVFGALFGMPVINQPQVAILGVGAVEKRPV
                     VVDDAIAIRHRAYLSLGYDHRLIDGAVADEFMSEVKKRIETFEASAL"
     gene            complement(7844..9262)
                     /gene="lpdA"
                     /locus_tag="PKK95_06700"
     CDS             complement(7844..9262)
                     /gene="lpdA"
                     /locus_tag="PKK95_06700"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOC17936.1"
                     /translation="MANEYDVVVIGAGTGGYPTAIRASQLGLKVAVVERQKALGGTCL
                     IWGCIPTKALLEHAHALKVAQGAKEWGLNVDAASLRLDMKQVQARKDKVVTTLTKGVE
                     FLFRKNKVDWIKGTARLAGGLKIEVAGEQPQTLVARKEIVVATGSQPRSVPGVEIDRK
                     RIITSDEAIGLREVPKSIVVLGAGAVGVEFASLFRTYGAEVTIVELLPRLVPVEDEAV
                     SADLQKAFRKRGIKTFTGSRVTSAQASESGVSLEVQTGDGKTEKMQADYLLVATGRGP
                     VTEGLGVKEAGLRLERGYIVVDEYYRTGVPGISAVGDVITFEKPGHPQLAHVSTAEGI
                     AVAERIAGQAPRTINYDQVPACTFCDPEIGSIGLSEQKARERGYDVAVSSFPFMALGR
                     AKIAGETEGFVKIVAEKKYDEVLGVHIIGPRATDLVAEASVALRLECTGEELVRTIHA
                     HPTFAEAVGEAAHGLHGGAIHI"
     gene            9361..10758
                     /locus_tag="PKK95_06705"
     CDS             9361..10758
                     /locus_tag="PKK95_06705"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC17937.1"
                     /translation="MAPRLKTLLIVDADPATRARLSGRLERHYRVLAAASAEAGLALL
                     AREEADLLLIDARLPGISGLEMLRIARENYALAQVVMVSAEGRVQSAVEAVKEGAYHF
                     LPKDVEVDELLAVLRNASDHQDLDRQVLALSAQVAETDREFVVGPSRAMREVIDLAQR
                     IARLSATVLVLGESGTGKELIARLIHRDGERPDGPFIPVNLAAIPRELVESTLFGHEK
                     GSFTGAYRQQLGKFELAAGGTLFLDEIGDLPSDLQGKLLRAIQEGEVERVGGTHPIRT
                     DFRLIAATNVDLDKSVKEGRFREDLYYRLNVIPIRMPPLRDRIEDLPDLAACFVRRYK
                     TRFRRSTEGFTPGAMKRLQAHWWPGNIRELENLVERLVAITEGEWITEDDLPLEYRVP
                     ATLPETGEEPPGGRLLQHACESFERSFILKALERAGWNVTAAARHLGIPLSTMKHKMD
                     RLAIRPLAKRLRAPQ"
     gene            complement(10833..11132)
                     /locus_tag="PKK95_06710"
     CDS             complement(10833..11132)
                     /locus_tag="PKK95_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOC17938.1"
                     /translation="MAEARRMGKSELFAHFAEKFDMKRAQARELFDELTVLAEKELKR
                     SGEFQLPGMVKLVVQKRKARMGRNPATGEAIKIPAKTVVKAKIAKPLKDSVLPRK"
BASE COUNT         1678 a         3934 c         3982 g         1713 t
ORIGIN      
        1 ggaactccgc ccacgagtcg agcaggcttg aagtcaggcc ttccgcggcc ccgcccccga
       61 gcgcacggct cgcgaccccg ctggcagccg ggcccacgag cgagatcagc ccgaacgtgt
      121 cgctgacgtc gccaagctgc acgtcctcgg agaacacgaa ctggtcgagc ctctgcagca
      181 cgtcggccgc gcgcgcgcga tcgagatcca tcaggatgag atcgcccagc tccagcagcc
      241 gcatgtcggc aatcatgcgc ccctggggcg tcaggaacgc cccgtaggtc ccgctgccgg
      301 gttgcagggg ggccacctcg caggtgagca tcgcgtgcag gtaggtgcgg cggtccgacc
      361 cggccaccac gagcttcccc cgcgccgccc ggtcgaggat cgccgaggcc gtccgggcgg
      421 cctggtaccc cgtcgaatcg tcatgaacgg ccatgcgtca cccggtgcta tccttctgaa
      481 gccatgcgca agcttcattc taccgttgtc ttcgcgctgc tcgccctggc accggccctc
      541 ctcgaggcac agcctctcgc gggcggcgga gtgcagttca cggtcttcct ccgcgggacg
      601 cccgtcggcg tcgaggaaac cacggtcgcg cgatcggccg acggcctcgt gatcaccggc
      661 acgtcccggc tcggtccgcc gctcgaggtg atcgtgcgcc gggcggagat caggtacgac
      721 gcgtccggca accccttgtc gtgcttcgtc gagggcagcc tccgccagcg gcagctggtc
      781 ctgtcgacca ccgtggaggg caacacggcg acgaccgatt tgaccgaggg cacctcgcgg
      841 tcccgtcagg cccacgagat cgcgccgggg gcggtgctgc tgtcgagcgt gtacttcggc
      901 ggtcacgagg cgttggcggc tcgcctgcag ggcaagaagg agggcgacga gatcccgatc
      961 tacgtgcccg ggcaaggcga ggcgaccgcc cgggtcgtct cagccaccga cgagcggatc
     1021 cagacctcga gcgggatgtt gaaggcccgc cggttccagg tggcgttgga gcagcagggg
     1081 aagaccaccg acaccgaggt ctgggtggac gaccagggac gtttggtacg gttctccacg
     1141 gcgtcgcaga cgatcgacgt ggtcaggacg gatgtcgcct cggtgtcggc ccggcgtgag
     1201 gcggtcgcga gacccggcga cgagtcggtg cgcatcccgg ccaacggctt cacactcgcc
     1261 ggcaccctct cgcggccggg ccagccggcc aagggagcga aacttcccgc gatcgtgctg
     1321 gtggggagca tcggggtgac cgaccgcgac gagtcgcgtg ccggcatccc gatcctgggc
     1381 cagctggcgt cggcgctagc cgacgcggga tacctcgtca tcaggtacga ccatcggggc
     1441 atcggtcaga gcggcgggcg cgccgagagc gcggagatcg gcgactacgc cgaggacgtc
     1501 cgatcggtcg tgcgcttcat gcgaaagcgc aaggacgtcg atcgcgaccg gctggcggtg
     1561 ctcgggtacg actacggcgg cagcgtggcg atgaccgcgg cggccaggga cggcgacatc
     1621 aaggcgctcg tcctggtggc gacgcccggc ctggctggcg gcgacctggt gctcgagcag
     1681 cagcgcgcgg cgctggcgaa gatggacctg accgaggccg agaagcagcg ccgcatcgac
     1741 ctgcagcagc ggatcaacga ggcggtcgtc agcggccgcg gctgggacca aatcccgccg
     1801 gcaatgcgcc agcaggccga gacggattgg ttccgcagcc tgctggcctt cgacccggcc
     1861 gagcaaatga gacgggtgcg ccagccggtg ctcgtcgtgc acggcgggct cgacgccgag
     1921 ctggggccgc accacgccga ggcactcgcc tccatggccg aggcgcgcaa gaatccggcc
     1981 ggcgagaagg tcaggctggc cgttgttcca ggcgtgaatc acctgctcgt gccgtcgcgc
     2041 gagggtacgg tggacgagta cgcatcgctc ggcggcgcga gtgtcagtcc cgaggtgacc
     2101 ggcgccatca cgacgtggct cggcgaggcg cttgccgcgg cgaagaagta ggcgtgacca
     2161 tgtggatact gagggggaac ggggccgacg gccagcaaac cctgacggtg cggctgatgc
     2221 cgggcaccgc gaggacgctt gggcgcggcc cccaggccaa cttcatcgtc gacgaggcgc
     2281 tggtctcgcg gctgcactgc cggttcgtgg tggacggggc ggggaagctc gcggtcgaag
     2341 acctgggaag caccaacgga acctgggtga acgaccaacg ggtcgaacgc gccgacctcg
     2401 ccccgggcga ccgcgtccgc gccggccaac tcgagctcgt cgtcgaaacg tcgcgcggcc
     2461 tgtgagcccc cttacggcct ttccccagtc aggaacgggt tcgtcatccg ctcctgcccg
     2521 atcatcgtcc gcgggccgtg cccgggatac acctccacgt cgtcggacag cttgaaaagg
     2581 acgccgcgga tcgagccaat cagcgtgtcg tggtcgcccc cgggcaggtc ggtccggccg
     2641 atcgagccgg cgaagagggt gtcgcccgag aacagcttct tgccaggccg acccgtgggc
     2701 cccaccagga ggctgacgcc gcccggcgtg tggcccggcg tgtgaaggac ctcgacccgg
     2761 taccgcccga actcgatggc ccccgggatc tcgtagaagc ggtcgacggg cggcggagtc
     2821 tcgatgttga agccgaagaa gcgcgcctgc tcgagcgccg cgttgtacag cggcagatcg
     2881 tcggcgtgga gccagatggc ggcgtccagc tgcttcttcg ccgcggccag tccagccacg
     2941 tgatcgacgt gcgcgtgggt gagaaggatg tggcgcacct cgaggcgctg ttcatcgacg
     3001 accgcgagca gctgctccac ctcgtcgccc gggtcgatga gaacggcctg gcgcgtttcc
     3061 tcacagccga ggacgaagcc gttcttgtag aacggcggcg cttcccgggt ttcgaagatc
     3121 atgtcccgat cctagcagcc tgtgctcgag tttcccacag gcggccgagc cggtgcccac
     3181 ctcggctagg ccgccaggat ggatgccagg cgcgagctgt cgatgttggc cccggagacg
     3241 accaccgcgg ccgggccggg gcagcggggg atcgtaccgg ccaggacggc ggcaaccgcg
     3301 gtcgcgccgg ccccctcggc gaccaggtgc tcggcctcga ccaggccgcg gacggccgcc
     3361 tcgagcgcgg gttcgctggc gagcacgacc cggtccacga ggcgctgcac gatcgggaac
     3421 gcgatgttgt cgggatccat gttgccggcc aggccgtccg caagcgtggg cccgaccttc
     3481 acttccacga tccgccctgc ggcgagcgag gccgtgaacg ggtgggacgc ctcgacctcg
     3541 acacctatga cgcgcgccgc aggccgcagg gctttcagga cctgcgcgat gcccgcaatc
     3601 agacccccgc cccccaccgg cacgatcacc tgggaaacct ccggcaggtc ttcgaggatc
     3661 tcgagggcaa tgccaccgat ggcggccagc aggtcggggt ggctgtaggg cgacaggtag
     3721 aaggcgcccg tgcggcgcgc gtgctccttg gcaagcgctt cggcgccctc gtagtcgtca
     3781 gcctgggccc gcagctcgga ggggtaacgc cggatggccg agagctttgc agccggcgcg
     3841 cttcgcggcg tgaagatgac caccggcagg cctatcgacg aggccgccca cgcgagcgca
     3901 cggccgtggt tgccggccga cgcggtgacc actctcggca gcggctcgcc cgggcgggtt
     3961 cgctcggcca gggcgagcac cgcgttgagc gccccgcgag ccttgaacga gctggtgacc
     4021 tgcagcgact cgagcttgag gtgaacgtcg gcccctgccc gttcgctcag ccagtcagag
     4081 cggatcagcg gcgtccgccg gagtcgcccc gccgtccgct gcctcgcccg ccgagcctcc
     4141 tccagaacgt ccatcgtgat tcctcgcggg gggcggcgga agccgcgccg gccacgtggc
     4201 ggagccgcct cagctggtcg atcaccgccc ccgcatcatg cggccgcatc tccaccgttt
     4261 tccgcagcat gccggcgacg gccgcgtcga acccgggcgg gaccgccggg ttgagcgagc
     4321 tcggggcggg aggcgtggcg tggaggatct tgaaggcggt ggccgagtcg gaaccgccgt
     4381 cgaagggcat ctgcccggtg accagctgga agagcagcac gccgagcgag aacaggtcgc
     4441 tgcgggtcac ggcgctttcg ccgagggcct cctcgggcga gacgtaggcg ccgggccggc
     4501 tggcagcccg gtcgtagtcc tcctcccgcc gctcctgtcc ctcggcagtc tgcggcgaga
     4561 ggccgaggcg gacctgcccc gcactggtcc aggcggcaag ccccacgtcg agcagcttgg
     4621 cctggtcgcg tggattgacg atgaccgagg tggccgagag cgcgcggtag atcaagccgt
     4681 gccggtggac gcgcgcgagc gcctcggcaa tctggagggc cagctcgagg gccgcctcgg
     4741 ggtcgagggg gcccttgtcc agcatggcgg cgagcgtttg ccctgggacg tactcgtggg
     4801 cgaggtagag gaagccgtcc tgctcaccga cgtcgaagag ggccgcgatg tgcggatgcg
     4861 aaacctcccg cgccttcccc gcatcggcga gaagggcggc acgccgcacg gggtcgccgg
     4921 caacttcctc gcggacgacc cggagcgcca ctgtccggcc ggtcacgttg tcgcgtgccc
     4981 gaaacaggtc gccgagcagt ccctggatgg agatagggtc caggatcgtg tatttctcag
     5041 tctgttccat gtcgccagcg ggcgcggaca tggtacacca gttcccatgc gagccatctt
     5101 cttcgacctg gtgggcacgc tcgtcgagcc gcgcggtccg gtcggggcgc agtacgcggc
     5161 aatcgcccgg cgtcacgggg agcatgcgga cgccgacgcc ctcggggcgg ccttcgcgcg
     5221 tgagatggcc tcgacgccgg cggtccaggc ctggggcttg tcgcgcgagg cggccgaggc
     5281 ggcggagcgc ggctggtggc acagcctcgt gcggcgggtc ctcgatctcg caggcgcggg
     5341 cggcctgctc gacgccgctc gcttcgagcc gttcttcgac gacctctacc gccacttcac
     5401 gacggccgac gcgtggcggc tctaccccga cgtgctgccg gcactcgacc gcctcgaaac
     5461 cgcggggatc gcgaccgggc tgatcacgaa ctacgattcc cgcatctacc ggctggtcga
     5521 gtcgctgggg ctcgcttcgt cgctcggctc gatcaccatc cccgcaagcg ccggcgccac
     5581 caagccggcg cgagccatct tcggccaggc gctgttctgc catcgcctgg ctgcatcaga
     5641 ggccgggcac gtgggcgact cgctggccga cgattaccag ggggcgctcg aagcgggcct
     5701 gcacgccgtg ctgctcgacc gcgacggcca tcacgccgcg ctggccggcg ttcgccgggc
     5761 tgggaacctg gaggaggcgg tggcgctgct ggaaaaggag ctgggctgaa ggcctgggcg
     5821 cacggcttca gccctgcgcg atctccgctt cgaagatctc cgcttcgaag acctccccga
     5881 aagccctcac cacggcgtcc tcgacctcac ccatcggcag ctcgcgcccc aggagcttcg
     5941 acagcgaggt gacgccgtgg tcgcggatgc cgcaggggac gatcaggttg aagtactcga
     6001 gccgcgtgtt gacgttgtag gcaaagccgt ggctggtgat ccagcgcgag atgcggacgc
     6061 cgattgcgcc gatcttgtcc gcgacgaccc aggcccccgt aaggccgccg atccggccgg
     6121 cctcgacgcc gaacgctgag gtgacgcgga tcatcacctc ttcgagatcg cgcacgtaac
     6181 gatgcacgtc ctgccggtcg ggccgcaggt cgaagatcgg gtaggcgacc agctgaccgg
     6241 ggccgtggta ggtgacgtcg cctccgcggc cggtctcgaa cagctcgacg ccgagctcgc
     6301 gcagccgctc gggcgtggca atcacgttcg accgcgcggc gtcgctccgg gcgccgagcg
     6361 tgatgacgtg gggatgctcg agcagaagca ggacgtcgcc aacgcggccc tggcgccgat
     6421 cgtcggccag ggcgcgctgc agttcgagcg cctccccgta ggggaccagc cccagacgcc
     6481 ggacgccgat ccgcctcaca gcgccgaggc ctcgaaggtc tcgatccgct tcttgacctc
     6541 cgacatgaac tcgtcggcga cggccccgtc gatgagccgg tggtcgtagc cgagcgacag
     6601 gtacgcccgg tgccggatgg cgatggcgtc gtcgacgacg acgggccgct tctcgacggc
     6661 accgacgccc aggatggcga cctgcggctg gttgatcacc ggcatcccga acagggcgcc
     6721 gaagacgccc gggttcgtga tggtgaaggt gccgccctgc acttcctcgg gcttgagctg
     6781 cttggcccgg gcgcgcgagg cgaggtcctg gatcgccttg ctcaggccaa ggatgttctt
     6841 ctcgtcggcg tgcttgatga ccgggacgat caggccccag tcgagcgcca ccgcgatccc
     6901 gacgttcacg tccttccggt agatgacgtt ctcgccgtcg atcgacgcgt tgaccaccgg
     6961 gacggcgcgc aggccgtcca ccaccgcctt gacgatgaag gagaggtagg tgagcttcgc
     7021 gccggcccgc tcgtactcgg ccttcttcgc ctggcgaagc ttttcgacgt tgccgaagtc
     7081 cacctcgaag accgagtgca cgtgggccga ggtgcgcttg ctcagcacca tgtgctcggc
     7141 gatgcgcctc ctcatcggcg tcatcggcac gacctcgtcg ccctcggcgg ccttcaccgc
     7201 ggggccgggg gaggcgggcg gcgcgggcgg cgcgggagcg ggcgccgcgg ggggcgcggc
     7261 gggcttcggc gactcgaggt aatcgagaat gtcctgcttg gtcacgcggc cgccgatgcc
     7321 cgacccctgc acctggctga tgtccactcc gtgctcggcc gcgatcttcc ggacgagcgg
     7381 cgacgaccgc tgccgccgca gttcgtcggc cgcggggcct tcggccgcgg cgtcgacggg
     7441 aacggccggc ggcggaggtg gcggcgcggc gggcgccggc tgtgcgggct ggatggccga
     7501 aggcggggga ggcggcggct gtgatggcgc tgcggcgcct tcggccggcg gcggcgccgc
     7561 ggcggccccc ttcccgggcg cctcgtcgat cacggcgacg acctggttga ccggcaccgt
     7621 ctcgccttcc ttcgaccgga tctcggtgac gacccccgcc gtgggggacg ggatctctgc
     7681 atcgaccttg tcggtggaga tctcgaacag cggctcgtcg cggtcgacct tgtccccgac
     7741 gcgcttgagc caccggacga tggttccctc ggcgatcgac tcgcccatct gaggcatcag
     7801 aacgttggtc ggcatgatgg tctccgcttc ggccgccgct cttctagatg tgaatcgcgc
     7861 ccccgtgcag gccgtgcgcc gcttccccga ccgcctcggc gaaggtcggg tgcgcgtgga
     7921 tcgtgcgcac cagttcctcg ccggtgcact cgaggcgcag cgcaaccgac gcctcggcca
     7981 ccaggtcggt ggcgcgaggc ccgatgatgt gcacccccag cacctcgtcg tacttcttct
     8041 cggcgacgat cttgacgaac ccttccgtct cgccggcaat cttcgcgcgg ccgagcgcca
     8101 tgaacgggaa gctcgagacg gccacgtcgt acccgcgctc cctggccttc tgttcgctga
     8161 ggccgatgct gccgatctcg gggtcgcaga aggtgcaggc cggcacctgg tcgtagttga
     8221 tcgtccgcgg cgcctggccc gcgatccgct cggccaccgc gatcccctcg gccgtcgaga
     8281 cgtgggccag ctgcgggtgc cccggcttct cgaaggtgat cacgtcgccc acggcggaga
     8341 ttcccggaac gcccgtgcgg tagtactcgt ccacgacgat gtagccgcgc tcgaggcgga
     8401 gtcccgcctc cttgacgccg agcccctcgg tgaccggtcc ccggccggtc gccacgagca
     8461 ggtaatcggc ctgcatcttc tcggtcttgc cgtcgccggt ctgcacctcg aggctgacgc
     8521 ccgattccga cgcctgggcg ctggtgaccc gcgagccggt gaacgtcttg atgccgcgct
     8581 tcctgaacgc cttctgcaga tcggccgaca ccgcttcgtc ctcgaccggc accaggcggg
     8641 gcagcagttc gacgatggtg acctcggcgc cgtaggtgcg gaagagggag gcgaactcga
     8701 cgcccacggc gccggcgcca aggaccacaa tcgacttcgg cacctcgcgc aagccgatcg
     8761 cctcgtcgct ggtgatgatc cgcttgcggt cgatctcgac gccgggtacg ctccggggct
     8821 gcgacccggt ggcgacgacg atttccttcc tggcgaccag cgtctggggc tgctcgccgg
     8881 ccacctcgat cttgaggcct ccggccagcc gtgcggtgcc cttgatccag tcgaccttgt
     8941 tctttcggaa caggaactcg acccccttgg tgagcgtggt gacgaccttg tccttccgtg
     9001 cctgcacctg cttcatgtcg aggcgcagcg aggccgcgtc cacgttcagg ccccactcct
     9061 tggccccctg cgccaccttc agcgcgtggg cgtgctcgag cagcgccttg gtcgggatgc
     9121 agccccagat caggcaggtg ccgcccagcg ccttctggcg ctcgacgacg gccaccttca
     9181 gacccagttg agacgcacgg atggccgtcg ggtacccgcc cgtgccggcc ccgatgacga
     9241 ctacgtcgta ctcgttcgcc acggtgactc ctcggtgatg gtcgtgtcgc aagcccgatt
     9301 ctaaacttat cccagaccag tccacgaagt ccacggagtc tggtctacaa ttcgcgcgtg
     9361 atggccccgc gcctcaagac cctcctcatc gtggacgccg acccggccac gcgcgcgcgg
     9421 ctgtcgggac ggctcgaacg gcactaccgc gtgcttgccg cggccagcgc cgaggccggc
     9481 cttgccctgc ttgcccgcga ggaggccgac ctgctgctga tcgatgcgcg gctgcccggc
     9541 atcagcggcc tggagatgct gcggatcgcc agggagaact acgccctcgc gcaggtcgtg
     9601 atggtgtcgg cggagggacg cgtccagtcg gccgtcgagg cggtgaagga gggggcctac
     9661 cacttcctgc cgaaggacgt tgaggtggac gagctgctcg ccgtgctgcg caacgcgagc
     9721 gatcaccagg acctcgatcg gcaggtgctc gcgctcagcg cgcaggttgc cgaaaccgat
     9781 cgcgagttcg tcgtcgggcc gagccgggcc atgcgcgagg tcatcgacct ggcgcagcgc
     9841 atcgcgcggc tgtcggcgac ggtgctcgtg ctcggcgaga gcggcacggg caaggagctg
     9901 atcgcccggc tgattcaccg cgacggcgag cgccccgacg gcccgttcat cccggtgaac
     9961 ctcgcggcca tcccccgcga gctggtggag agcacgctgt tcgggcacga aaaggggtcg
    10021 ttcaccggcg cctaccggca gcagctcggg aagttcgagc tggccgccgg cggcacgctg
    10081 ttcctcgacg agatcggcga cctgccctcc gacctgcagg gcaagctgct gcgtgccatc
    10141 caggagggcg aggtcgagcg cgtcggcggc acgcacccga ttcgcaccga cttccggctg
    10201 attgccgcga ccaacgtgga tctcgacaag agcgtgaagg aggggcgctt ccgcgaggac
    10261 ctgtactacc ggctcaacgt gatcccgatc cgcatgccgc cgctgcgcga ccggatcgag
    10321 gaccttcccg acctggccgc ctgcttcgtc cgccgctaca agacgcggtt ccgccggagc
    10381 acggaaggct tcacgcccgg ggcaatgaag cggctgcagg cccactggtg gccgggcaac
    10441 atccgcgaac tcgagaacct cgtcgagcgg ctcgtggcga tcaccgaggg cgagtggatc
    10501 accgaggacg acttgccgct cgagtaccgg gtgcccgcga cgctgcccga gacaggagag
    10561 gagccgcccg gcggcaggct gctccagcac gcgtgcgaga gcttcgagcg aagcttcatc
    10621 ctgaaggcgc tcgagcgcgc cgggtggaac gtcacggcgg ccgcccgcca cctcggcatc
    10681 ccgctgagca cgatgaagca caagatggac cggctcgcga tccggccgct cgccaagcgc
    10741 cttcgggcgc cgcagtaggg gagaaagaga aaggccccgg agagcgcaga tggcactcgc
    10801 ccgaggcctt cacgcgtcaa gccgcgcgcg tgctacttgc gggggagaac gctgtccttc
    10861 agcggcttgg cgatcttcgc cttgacgacc gtcttggcgg gaatcttgat cgcttcgcca
    10921 gtagccgggt tgcggcccat ccgcgccttg cgcttctgga ccaccagctt caccatgccg
    10981 ggcagctgga actcgccgga acgcttcagt tccttctctg caagcacggt gagttcgtcg
    11041 aacaactccc gagcctgcgc gcgcttcatg tcgaacttct cggcgaagtg cgcgaacagc
    11101 tcggatttgc ccatcctgcg ggcctctgcc atcggcatcc ccctgttggg cgcgctgaac
    11161 gatcacccgc cgtcaaccac gcgcgcgagc agttgtgcgg aaactaagcc aaatttcgag
    11221 gtatcctagc gcgcccgttc cgagcttgtc aattggatcg caagcatttt cgcgggttcc
    11281 cgcacagatc gccagcgtag aatcgga
//
LOCUS       DAOPQX010000088        11186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6686_length_11186_cov_4.896326, whole genome shotgun sequence.
ACCESSION   DAOPQX010000088 DAOPQX010000000
VERSION     DAOPQX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6686_length_11186_cov_4.896326"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..2232
                     /locus_tag="PKK95_06715"
     CDS             46..2232
                     /locus_tag="PKK95_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOC17939.1"
                     /translation="MTAPDRAEAANRYCDRLHIPVPDLDVAVRYPTLSVADLMALAVL
                     EAGRPLAVDEIAARLARLDLPTRLRRAGDPAALPKAWRGQGPLVRDPVNGLFYLDLLS
                     HYEIGHLAFVGNAERQAAVMREPPQVSQPPDTVPLSQEEVDTAFEGRDLGPFSSVSSL
                     RQAAAILEAAGGGPLSLDEVNDRLTRFSQFARLDERAVKAWRTNLVRVEDDGSLRLER
                     SSPDLPGMRRDIRRLAVSTLRERAWSAGFQSKEAELDIEREARDRRDMEEARRTRRAF
                     LHVVVLDRIARAAAVIDVARRELRSFIGSALREVPAHLEGYDFLAGLDLRPSLRALGL
                     DPDRWWLAELRPSQRTFRPAGTGRALPVELRSIVQATTNRRGAPANPKEWKSLLTGRS
                     PARLEARLARDADALFALYEYGSLHGGVRMRRRQGEALLPVSWSMRGDFDFWSVVEAS
                     IRRLVPVHLVVGYPAGLSPEPYKDAVPVTIVEREMNGRTLYVRAGEDGVIAMDRADVA
                     AIGLPATATAAIESLRPAHSHRGESRTCRLLVTLHGISPPIWRRLQVEASMTLEKLHD
                     VIQAAFGWTDSHLHAFLAGDERIGIPHDLDDIATGLVTRSGRLVRLADLVDLGFTRFA
                     YEYDFGDSWMHTIEVEEVRPGREEGDAWARCLEGARACPPEDCGGVRGYEQLLEILFD
                     PRHPEFEQTRRWAARFEPERFELREAKRGDRRPEAVLKSRRRTGPS"
     gene            complement(2229..3260)
                     /locus_tag="PKK95_06720"
     CDS             complement(2229..3260)
                     /locus_tag="PKK95_06720"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOC17940.1"
                     /translation="MPSPNPGPAELLAALARALSSLAVPWYLFGAQAAVIWGRPRLTA
                     DVDVTVRLPADDHGPLVRVLEEAGFTLRTGDPDEFVRRTRVLPFVHRPSQLPVDVVLA
                     GPGLEEAFLSRAVPVTIAGVSIPVISPEDLVVVKILAGRPKDIEDVRGILAERRASLD
                     VDSVRATLRQLEEALGRTDLLRSLQSELDQDLLPAATSGLASVAVDQDDLELAFNFVS
                     SAGPFDNQAFVSLETGAIYWQTEDEELPDDVDDGDRYVAVPHKNDLDLGRRLALRFGE
                     ERLPNRYEEIAEAFRHRGAYGRFKRILESAERLEDWYTFEAAETARALREWCAEHGLR
                     LTRRPGRER"
     gene            complement(3232..3486)
                     /locus_tag="PKK95_06725"
     CDS             complement(3232..3486)
                     /locus_tag="PKK95_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17941.1"
                     /translation="MTRDDILAFARRDWAAVTDAKARFWAGRKATMTASEALACADML
                     RRHVRALKPGWPDARERAEDLDLHLRLSEALRAVPEPWAR"
     gene            complement(3559..5472)
                     /locus_tag="PKK95_06730"
     CDS             complement(3559..5472)
                     /locus_tag="PKK95_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17942.1"
                     /translation="MAPIPRHARVMVLAFVLAGLAPATGWQEGWPARPAKAAAVFEID
                     MWPGEGRPDLVAGSAALVPRAEPHRDAPAAPVLHVEKGQALHFDETLHRTTLAGRLRA
                     REDSHVAGRRLGPVSRLSRDDYYSDRVPTVSLPVPAGLVIDYLQSGAEGGCLVRVRGE
                     VIDTDPCPDLVGDPPGFALESEPRTEWWVRVLIDGRPVGWLLVDGKKVRERGRTFAPR
                     PSPALRMSPARLVGLVFQRGRVMKYPPLAVVTLVAAFVSLPAANGATSGPSAASGPGA
                     TSVPTVTSGLGAAQRVAPGDLWFGTISPTHTGRIHSGCKQFADPEEVVGKGPITRVAV
                     YGTEFIHGIRLRYGSDGIGLSHGFTEPVSGLSWAEWDVPDGERITRVEGAIARDYIVW
                     LRFVTDRGTEREFGKKRGRTFVVSDPGGGALRTISGWANHKRHPSLYRAVTSLTFQFG
                     APYFIKKIDFDEKALDLARLKAAPEQCASQDFTNETSVEQAASYTNTLTLAKETRVTF
                     ESAFGLRFTTEAWIEAGGKLVKGGGSMSFESSAEVRSGRSFSSSREEQVSWSVPVRVP
                     PHSRVVASSTWRKYPVSIPFTYTVAWYVGSKDNIKKEVKLPGLYEDVRVDDLKHDFKE
                     VKLTGEPGRRGRE"
     gene            5646..5963
                     /locus_tag="PKK95_06735"
     CDS             5646..5963
                     /locus_tag="PKK95_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17943.1"
                     /translation="MPKTAISVTLDVENITWLRGQVRAAGLRSVSELLDRLVSAARES
                     GRIGPPRSVVGTIDIAASDPLLEGADAAVRKQFDGVLGVRARVAEPHGEYRVPSKSRK
                     RGG"
     gene            5956..6363
                     /locus_tag="PKK95_06740"
     CDS             5956..6363
                     /locus_tag="PKK95_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOC17944.1"
                     /translation="MADLQAAVTDTHALLFHAAGGRALGKKAAAFFARAERREAIVYV
                     PAAVMWECSLLARVGRVNLRRSVRGFFDDLFSNPAYQPLDLTPAEVCLADELRFTRDP
                     FDALIVAAACQANLPLITRDAVIRSSGTVSVVW"
     gene            complement(6376..8223)
                     /locus_tag="PKK95_06745"
     CDS             complement(6376..8223)
                     /locus_tag="PKK95_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC17945.1"
                     /translation="MTSNYSRPLSLALAAAVVLACSALGATLLREGSRSDLQNGAWPQ
                     SRSQAASADICIYGGTASAVIAAVQARAMGKSVIVVSPDAHLGGLTSGGLGWTDTGNK
                     AVIGGLSREFYRRVWKHYDRPEAWTWEPRESFGNRGQGTVAMDGELRTMWIFEPHVAD
                     KVFEELVREHQIPVRRNERLDRAKGVRKDGARIVSITTLSGTTYTARMFIDATYEGDL
                     MAAAGVDYHVGREGRDVYGEQWNGVQTGVLHHRHHFGPFPPSAPAAAATAAAEAPGRP
                     GAAHKPPRLWEISPYVVPGDPTSGVLPRVSAEPPGEYGSGDRRVQAYCFRTCLTDHPD
                     NRLPFPKPAAYDPKQYELLLRVFQAGWRETFDKFDRIPNRKTDTNNHGPFSADNIGFN
                     YDYPEASYERRVEIVQEHKDYQQGLLYFIANDPRVPKEVQEEMRRWGLARDEFRDSGG
                     WPHQLYVREARRMVGQYVMTENDLLEKRPTPEPVGMGSYTIDSHNVQRYITPDGYVQN
                     EGDIGVATPGPYEIAYSSLVPKRGQADNLLVPVCVSSSHIAFGSIRMEPVFMILGQSA
                     ATAAAMAIDDGVAVQEVSYAKLRARLLADGQVLEWAAPSAHARSFPTAQ"
     gene            8305..9192
                     /locus_tag="PKK95_06750"
     CDS             8305..9192
                     /locus_tag="PKK95_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOC17946.1"
                     /translation="MNRSSTTAIVLLALTLSGGRAEAQQSKPAYPPHTIPNSQLRVLP
                     VNAAGRHYQLHVGLPASYSTDAVRRYPVVYVTDAYWDFQKMDAIRGGLVYDKVAPEFI
                     IVGLGYAGENLNYGQLRSWELSPAPFGGGGADASGHAADFLRTIETEIIPLIEREYRA
                     DPSFRVLAGASLGGLFTLYSMYARPGLFHAYVAATPAVVVGNDWLLGFEEKLAKATKQ
                     LKARLFVSGGGNESPAFLSGILRFNQRIASRKWPGLAYEFRIIDGERHAGMQLESYNR
                     GLRFVFAPLAPETGPTPGR"
     gene            9435..10085
                     /locus_tag="PKK95_06755"
     CDS             9435..10085
                     /locus_tag="PKK95_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOC17947.1"
                     /translation="MQLIDQLRAEHDLIEQVAGAFRTFARQRTRGEGRAADGTRFLAF
                     FRLYAGSFHHGREEDTLFVALQRDAELPGDRGPIAALLDDHHRMGGLLDRLEAVADSD
                     PAAPDLAALALDYSEQLWHHIDAENSVLLPESGQRLRRHGMHELPSRALTPAESAARD
                     DGRALLLEYPPVHEADVPRGDGCVMCPAFARDCEGLERTWWNEHEWDEFEDHLPSG"
     gene            complement(10168..>11186)
                     /locus_tag="PKK95_06760"
     CDS             complement(10168..>11186)
                     /locus_tag="PKK95_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOC17948.1"
                     /translation="TFALVSLVGALAITGCGGGSGTPPAEKGPERVKVGLLLDNVHER
                     WGRDRDMIQEIVTDMGGDLLVEVADSDHARQVQQARKMLAEGVKSLIIVAHDTEKAAE
                     IVEKANAQKVPVIAYDRLIQNADLALYVGFDVPRIGTLQASHILSAAPKGNYILIGGS
                     PTDDNAKILREAQMKVLEPAIKKGAIRVVADGWAENWTAEAAAKITEEGLAKARGQVA
                     AIVASNDVTAGGAIGVLEARGLAGKVAVSGQDAELEAARRIVAGTQTMTVYKSLRTMT
                     RLAARNAVLLAKGEKIDTATAVNNGKMDVPAMMFDPIAVDKDNLEGLLIGDGFLTREE
                     VFGK"
BASE COUNT         1676 a         3888 c         3872 g         1750 t
ORIGIN      
        1 gcgcgagcgc caacgattgt gccccaacga cgttacgata gcgcgatgac cgccccagac
       61 cgtgccgaag ccgccaaccg ctactgcgac cgcctccaca tcccggtgcc cgacctcgac
      121 gtcgcggtgc gatatcccac gctcagcgtg gccgacctga tggcgctcgc ggtgctcgag
      181 gcaggccgac cgctggcggt tgacgagatc gccgcccggc tcgcacggct cgaccttccg
      241 acccgccttc ggcgagcggg cgatcccgcg gctctcccga aagcctggcg cgggcagggg
      301 ccgctcgtcc gcgacccggt gaacggcctc ttctacctgg acctgctctc gcactacgag
      361 ataggccacc tcgcgttcgt cgggaacgcc gagcggcagg cggcggtgat gcgcgagccg
      421 ccgcaggtga gccagccgcc cgatacggtg ccgttgtcgc aggaggaagt ggacacggcg
      481 ttcgagggcc gcgatcttgg tcccttctcc tccgtgtcgt cgctgcgcca ggcggccgcc
      541 atcctcgaag cagccggcgg aggcccgctc tccctcgacg aggtcaacga ccggcttaca
      601 cgcttcagcc agtttgcgcg tctcgacgaa agagcggtca aggcctggcg aaccaacctc
      661 gtgcgcgtgg aagacgatgg gtcgctgcgc ctcgagcgct cgtcacccga cctgccgggc
      721 atgcggcgcg acatccgccg cctggccgtc tccacgctgc gcgagcgcgc ctggtcggcg
      781 gggttccagt cgaaggaggc ggagctggac atcgagcgcg aggcccggga ccggcgtgac
      841 atggaagaag caaggcgcac gcgccgcgcg tttctccacg tcgtcgtcct cgacaggatc
      901 gctcgagccg ccgccgtgat cgatgtggcc cgccgggagc tgcgctcgtt catcggcagc
      961 gcgctgaggg aggtccccgc gcacctcgag ggctacgact tcctcgcagg cctcgatctc
     1021 cgcccctcgc tgcgcgccct cggcctcgac ccggaccggt ggtggctggc cgagctgagg
     1081 ccgtcgcagc gaaccttccg cccggccgga accgggcgcg cgctgccggt agagctgagg
     1141 tcgatcgtgc aggcgacgac gaaccgccgc ggagcgccgg ccaacccgaa ggagtggaag
     1201 agcctgctca ccgggcgatc gccggcgcgc ctcgaggcgc ggctcgcccg cgacgcggac
     1261 gcgctgttcg cgctctacga gtacggctcg ctgcacggcg gcgtgcggat gcgccgccgg
     1321 cagggcgagg cgctgctgcc ggtttcgtgg tcgatgcgcg gggacttcga cttctggtcg
     1381 gtggtggaag cctccatccg gcgcctggtc ccggtccacc tcgtcgtcgg ctatcccgcc
     1441 ggcctgtcgc cggagccgta caaggatgcg gtcccggtca caatcgtcga gcgcgagatg
     1501 aacgggcgta cgctgtacgt ccgggctggc gaagacggcg tcatagcgat ggatcgagcc
     1561 gacgtcgccg ccatcggcct gcccgccacg gccaccgcgg cgatcgagtc gctccgcccg
     1621 gcacacagcc accgcggcga gagccgcacc tgccggctgc tcgtcacgct gcacggcatc
     1681 tccccgccaa tctggagacg cctgcaggtg gaggcgtcga tgacgctcga gaagctgcac
     1741 gacgtgatcc aggccgcgtt cggctggacg gacagccacc tccacgcctt cctggccggc
     1801 gacgaacgga tcggcattcc ccacgacctg gacgacatcg cgacgggcct cgtcacgcgc
     1861 agcggccgcc tcgtgaggct cgcggatctc gtggacctcg ggttcacccg gttcgcctac
     1921 gagtacgact tcggcgattc gtggatgcac acgatcgagg tggaggaggt gagacccggg
     1981 cgtgaggagg gcgacgcgtg ggcgcgctgc ctcgagggcg ccagggcctg cccgccggag
     2041 gactgtggcg gcgtgcgagg ctacgaacag ctgctcgaga tcctgttcga cccgcgacac
     2101 ccggagttcg agcagacacg ccgatgggct gcgcgcttcg agccggaacg gttcgaactc
     2161 cgcgaggcga aacgcggcga tcgccgccct gaggcagttc tgaagagccg gcgccggaca
     2221 gggcccagct aacgctcgcg gcctgggcgg cgcgtcagcc gaaggccgtg ctcggcacac
     2281 cattcccgca gcgcgcgcgc ggtctctgcg gcctcgaagg tgtaccagtc ttcgaggcgt
     2341 tccgccgact cgagaatccg cttgaagcgc ccgtacgcgc cgcggtgccg gaacgcttcg
     2401 gcgatctcct cgtaccggtt cgggagccgc tcttccccga agcggagcgc caggcggcgg
     2461 ccgaggtcga ggtcgttctt gtggggaacg gcgacgtacc ggtcgccgtc atcgacgtcg
     2521 tcgggaagct cttcgtcctc ggtctgccag tagattgcgc cggtctcgag ggacacgaac
     2581 gcctggttgt cgaaggggcc ggcggagctg acgaagttga acgccagctc gaggtcgtcc
     2641 tgatcgacgg ccaccgatgc gaggccgctc gttgcggcag ggagaaggtc ctgatccagc
     2701 tcgctctgta acgagcgcag caggtcggtc cgtccgagcg cctcctcgag ttgcctcagc
     2761 gtagcgcgga ccgaatcgac gtcgagagag gctcggcgct cggcgagaat gccgcggacg
     2821 tcctcgatgt ccttcgggcg ccccgcgaga atcttcacca cgacgagatc ctcggggctg
     2881 atgaccggga tgctgacacc ggcgatcgtg acgggaaccg cgcgcgagag gaacgcctcc
     2941 tcgaggcccg gacccgcaag gacgacgtcc acgggcaatt gactgggacg gtgaacgaag
     3001 ggcaggacgc gagtgcggcg cacgaactcg tcagggtcgc cggtgcggag ggtgaagccg
     3061 gcctcctcga gcaccctcac gagcgggccg tggtcgtcag cgggcaaacg gacggtgacg
     3121 tccacgtcgg ccgtcagcct cggccgcccc cagatgacgg ccgcctgcgc gccgaagagg
     3181 taccagggca ccgcgaggga cgagagggcg cgcgccagcg cggcgaggag ttcagcgggc
     3241 ccagggttcg gggacggcac gcagcgcctc cgagagtcgc aaatgaagat ccaggtcctc
     3301 cgcgcgttcc cgggcatccg gccagccagg cttcagggcg cgaacgtgcc ggcggagcat
     3361 gtcggcgcag gccagggcct cgctggcggt catggtcgcc tttcgtcctg cccagaagcg
     3421 cgccttggcg tcggtcaccg cggcccagtc gcgtcgggcg aaggccagga tgtcgtcgcg
     3481 ggtcatccga ttccgcatga tagcagaggc gatcacgagg gtgtccccgc gacgccggaa
     3541 aaccggcagg agtccttatt actcccgacc ccgcctgccc ggctcgcccg tcagcttgac
     3601 ctccttgaag tcgtgcttca ggtcatcgac gcgcacgtct tcgtagagcc ccgggagctt
     3661 cacttccttc ttgatgttgt ccttcgagcc gacgtaccag gccacggtgt aggtgaaggg
     3721 gatgctgacc gggtacttgc gccaggtcga gctcgccacg acacgggaat gcggcggcac
     3781 ccgcaccggc acggaccagg acacctgctc ctccctcgag cttgagaacg agcggccgga
     3841 cctgacttcg gcagacgact cgaaggacat actgccgccg cccttgacga gtttgccgcc
     3901 tgcctcgatc caggcctccg tcgtgaacct caacccgaaa gccgattcga aggtcaccct
     3961 cgtttccttc gccagcgtga gcgtgttcgt gtagctcgcc gcctgctcga cggaggtctc
     4021 gttcgtgaag tcctgcgacg cgcattgctc gggcgccgcc ttcagccggg cgaggtcgag
     4081 cgccttctcg tcgaagtcga tcttcttgat gaagtagggg gcgccgaact ggaacgtcag
     4141 gctggtgacg gcgcggtaca gcgacgggtg acgcttgtga ttcgcccacc cgctgatggt
     4201 ccgcagcgcg ccgcctcccg ggtcggagac gacgaacgtc cgcccgcgct tcttgccaaa
     4261 ctcccgctca gtcccgcggt cggtgacgaa acgcagccag acgatgtagt ccctggcaat
     4321 cgcgccctcg acgcgcgtga tccgttcgcc atccggaacg tcccactccg cccacgagag
     4381 gcccgacacc ggctcggtga aaccgtggga gagcccgatc ccgtcgctcc cataccgaag
     4441 acggatcccg tggatgaact cggtcccgta gacggcaacc ctcgtgatcg gacccttgcc
     4501 gaccacctcc tcgggatccg cgaactgctt gcagcccgag tggatcctgc cggtgtgcgt
     4561 cgggctgatg gttccaaacc acaggtcccc gggtgccacc cgctgcgccg cgcccaagcc
     4621 ggacgtcacg gtgggaacgg aggtcgcgcc tgggccggat gccgcgctcg gaccggatgt
     4681 cgcgccgttc gcggccggca gtgaaacgaa ggcggcgacg agggtgacga cggcgagcgg
     4741 cggatacttc atgacgcgcc ccctttggaa aacgagaccc acaagccggg caggcgacat
     4801 gcgcagggcg ggcgacgggc gaggcgcgaa ggtccgtcct ctttcccgca ccttcttgcc
     4861 gtcaaccagg agccagccaa cgggtcgccc gtcgatcagc acgcgcaccc accactccgt
     4921 ccgcggctcc gattccaggg cgaagccggg gggatcgccg acgaggtcgg ggcagggatc
     4981 ggtgtcgatg acctcaccgc gaacacgaac gaggcagccg ccttccgcgc cggactgcag
     5041 gtagtcgatc accaggccgg caggaacggg gagggagact gtgggaaccc ggtcgctgta
     5101 gtagtcgtct cgggagagcc gggacaccgg acccaggcgg cgcccggcga cgtgcgagtc
     5161 ctcgcgcgcc ctcaaccttc ccgccagcgt ggtccggtgc agggtttcat cgaagtgcag
     5221 cgcctggcct ttctcgacgt gcagcaccgg ggcggcgggc gcatcccggt gcggctcggc
     5281 gcgcggcacc agggcggcgg agcccgccac cagatccgga cgaccctcgc cgggccacat
     5341 gtcgatctcg aagacggctg cagccttggc aggacgcgcg ggccagccct cctgccagcc
     5401 agtcgcgggc gccaggccgg caagaacgaa tgcgagcacc atcacgcgag cgtgcctggg
     5461 gatgggtgcc atgacgggga tcgcgcggcg accggcacat gctagcacgc agcacgggcg
     5521 tcacgagggt cacctgcaca gtcacctgca caagaacgac cggcatgtgg acggcgacag
     5581 gccacttctg ggccgcgggc ccacacccgc tggacgatac gataaacata cgatatagtc
     5641 gtgtcatgcc caagacggcg atctccgtca cgctcgatgt cgagaacatc acctggctca
     5701 ggggccaggt cagggcggcg gggcttcgca gcgtgagcga actgctcgat cgcctggtca
     5761 gcgcggcgcg cgagtcgggc cggatcggtc cgccccgctc ggtagtggga acgatcgaca
     5821 tcgccgcctc cgacccgctg ctggaagggg cggacgccgc cgtcagaaag cagttcgacg
     5881 gcgttctcgg tgtccgggcc cgggtcgccg agccgcacgg cgagtaccgg gtgcccagca
     5941 agtcgagaaa acgaggtggc tgacctgcaa gccgccgtca ccgacacgca tgccctgctg
     6001 tttcacgcgg ccgggggacg ggcacttggg aagaaggcgg cggccttctt tgcccgcgcc
     6061 gaacgccgcg aggcgatcgt ctacgtcccc gctgcggtca tgtgggagtg cagcctgctg
     6121 gctcgcgtcg gccgcgtgaa cctgcgccgg agcgtccggg ggttcttcga cgacctcttc
     6181 agcaacccgg cctaccaacc gctcgacctg acgccggcgg aggtctgcct cgccgacgaa
     6241 ctccgattca cccgcgaccc gttcgatgcc ctgatcgttg ccgcggcctg ccaggccaac
     6301 ctgccgctca tcacgcgaga cgccgtgatt cgctcttcgg gcaccgtcag cgtcgtctgg
     6361 taaggcggtg acgcctcatt gggccgtggg gaagctgcgc gcgtgcgctg agggtgctgc
     6421 ccactccagc acctggccgt cagcgagcag ccgggcgcgg agcttcgcgt agctcacctc
     6481 ctgcaccgcc accccgtcgt cgatcgccat cgcggccgcc gtggccgccg actggccgag
     6541 gatcatgaac accggctcca tgcggatcga cccgaacgca atgtgcgagc tggacacgca
     6601 gaccggcacc agcaggttgt cggcctggcc ccgcttcggc accagcgagc tgtacgcgat
     6661 ctcgtagggc cccggcgtcg cgacgccgat gtcgccctcg ttctgcacgt acccgtccgg
     6721 cgtgatgtag cgctgcacgt tgtgcgagtc gatggtgtat gagcccatgc cgaccggctc
     6781 gggcgtgggc cgtttctcca gcaggtcatt ctcggtcatc acgtactggc cgaccatgcg
     6841 gcgggcttcg cggacgtaga gctggtgcgg ccagcccccg ctgtcgcgga actcgtcgcg
     6901 cgcgagcccc caccgccgca tctcttcctg cacctccttc ggcacgcgcg ggtcgttggc
     6961 aatgaagtag agcagcccct gctggtagtc cttgtgttcc tggacgatct cgacacgccg
     7021 ctcgtacgac gcctcggggt agtcgtagtt gaagccgatg ttgtcggcgc tgaagggacc
     7081 gtgattgttc gtgtcggtct tgcggttggg gatgcggtcg aacttgtcga acgtctcgcg
     7141 ccagccggcc tggaagacgc gcaggagcag ctcgtactgc ttcgggtcgt aggcggccgg
     7201 cttcgggaac ggcaggcggt tgtcgggatg atcggtgagg cacgtgcgga agcagtaggc
     7261 ctgcacgcgc ctgtcgccgc tgccgtactc gcccgggggc tcggcgctga cgcgcggcag
     7321 cacgccgctc gtgggatcgc ccggcaccac gtaggggctg atctcccaca ggcgcggcgg
     7381 cttgtgcgcg gcgccgggcc ggccaggcgc ctctgccgcg gccgttgccg cggcggccgg
     7441 ggccgagggc gggaaggggc cgaagtggtg gcggtggtga agcacgccgg tctgcacgcc
     7501 gttccactgc tcgccgtaga cgtcccgccc ctcccggccc acgtggtagt cgacgccggc
     7561 ggccgccatc aggtcgccct cgtacgtggc gtcgatgaac atcctggcgg tgtaggtcgt
     7621 cccgctcaac gtcgtgatcg acacgatgcg cgcgccgtcc ttccgcaccc ccttcgcgcg
     7681 gtcgagccgc tcgttccggc ggacggggat ttgatgctcc ctgacgagct cctcgaaaac
     7741 cttgtccgcg acgtgcggct cgaagatcca catcgtccgc agctcgccgt ccatcgcgac
     7801 ggttccctgg ccccggttgc cgaagctctc gcgcggctcc cacgtccagg cttccggcct
     7861 gtcgtagtgc ttccacacgc ggcggtagaa ctcgcgcgac aggccgccga tgaccgcctt
     7921 gttgccggtg tcggtccagc ccagaccgcc gctcgtcagg ccgcccaggt gggcgtcggg
     7981 cgagacgacg atgaccgact tgcccatggc cctggcctgg acggccgcga tcaccgcgct
     8041 cgccgtgccg ccgtagatgc agatgtcggc gcttgccgcc tgggaccgcg attggggcca
     8101 ggcgccattc tgcaaatcgg agcggctgcc ttcccgcagg agtgttgcac cgagggccga
     8161 gcaggcgagg acgaccgcgg ccgcgagggc gagtgaaagg ggtcgggagt aattagaagt
     8221 catgccgggt ttccggcagg caggggtcga gagtatcgcc atggccgata gcgtacaccc
     8281 ctttccgggt tatgctttgc ggccatgaac aggagctcaa cgaccgccat cgtccttctt
     8341 gccctgacgc tgtcgggggg gcgcgccgag gcacaacagt cgaagccggc gtacccgccg
     8401 cacacgatcc cgaactcgca gctccgggtg ctcccggtca acgcggccgg ccgtcactac
     8461 cagcttcacg tcggcctgcc cgcgagctac tcgaccgacg cggtgcggcg gtacccggtt
     8521 gtctacgtca ccgatgccta ctgggacttc cagaagatgg acgccatccg cggcggcctc
     8581 gtgtacgaca aggtcgcccc ggagttcatc atcgttggac tgggctacgc gggcgagaac
     8641 ctcaactacg gccagctgcg cagctgggag ctgtcgccgg cgcccttcgg cggcggcggc
     8701 gccgacgcct cgggccacgc ggccgacttc ctgcgcacga ttgagacgga gatcatcccg
     8761 ctgatcgagc gcgagtaccg ggccgacccg tcgttccgcg tccttgcggg ggcgtcgctg
     8821 ggcgggttgt tcacgctgta ctcgatgtac gcgcggcccg gcctgttcca cgcctacgtt
     8881 gccgccaccc cggcggtcgt cgtcggcaac gactggctgc ttggtttcga agagaagttg
     8941 gcgaaggcga ccaagcagct caaggcacgg ctgttcgtca gcggcggcgg caacgaatcg
     9001 cctgcgttcc tctcgggcat cctgcggttc aaccagcgca tcgcgtcacg gaagtggcca
     9061 gggctggctt acgagttccg catcatcgac ggcgagaggc acgctggcat gcagctcgag
     9121 tcgtacaacc gcggcctgcg tttcgtgttc gcaccgctgg ccccggagac cggtccgacg
     9181 ccgggacggt aggcacgccc tcagcgacgc gagcggccga cgtggggccg gcagacgcgg
     9241 ggccgtacca aacgtgtcgc attagtcacg gccgacgtgg ggccgtacca aagacgtcgc
     9301 gtttgtcacc agacgcgggg ccgtaccaaa catgtcgcgt tttgcacgcc tggggtacgg
     9361 cccccctgtg ctgtcgcgtt ttgcacgcct ggggtacggc ccccggtgcg tgtaggatgg
     9421 cgggaggggg cattttgcag ctcatcgacc agctgcgcgc cgagcacgac ctgatcgagc
     9481 aggtcgccgg tgcgttcagg accttcgcgc ggcaacgcac ccgcggcgag ggcagggcgg
     9541 ccgatggcac ccgcttcctc gccttcttcc gcctctacgc cggcagcttc caccacggcc
     9601 gcgaggagga cacgctgttc gtggcactgc agcgcgacgc ggagctgccg ggcgatcgtg
     9661 ggccgatcgc tgcgcttctc gacgaccacc accggatggg aggcttgctc gaccggctgg
     9721 aagcggtggc cgacagcgac ccggcggcgc ccgatctcgc cgcgctcgcg ctcgactaca
     9781 gcgagcagct ctggcaccac atcgacgccg agaactcggt gctgctgccg gagagcgggc
     9841 agcggctgcg ccgccacggc atgcacgagc tcccgtcgcg cgcgctgacc ccggccgaga
     9901 gcgccgcgag ggatgacggg cgcgcgctgc tcctggagta cccgccggtg cacgaggccg
     9961 acgtgccgcg aggcgacggc tgcgtgatgt gccccgcctt cgccagggat tgcgagggcc
    10021 tggagcgcac gtggtggaac gagcacgagt gggacgagtt cgaggatcac ctgccctcgg
    10081 ggtgaccggt gggcgactct cggcttcgtc cgcgcggtgc cggaaaaccg gcaccagttc
    10141 ttattgctcc cgaccccgat cgtacggcta cttcccgaac acctcttcgc gcgtcaggaa
    10201 cccgtcaccg atgagaaggc cctcgaggtt gtccttgtcc accgcgatcg ggtcgaacat
    10261 catcgccggc acgtccatct tgccgttgtt cacggcggtg gcggtgtcga tcttctcgcc
    10321 cttcgccagc agcacggcgt tgcgggcggc caggcgcgtc atggtccgca gcgacttgta
    10381 gacggtcatc gtctgcgtgc cggcgacgat gcgcctggca gcttcgagct cggcgtcctg
    10441 gcccgagacg gccaccttgc ccgccaggcc cctggcctcg agcacgccga tggcgccgcc
    10501 cgccgtcacg tcgttgctgg cgacgatggc ggccacctgg cccctggcct tggcgaggcc
    10561 ctcctcggtg atcttcgccg cggcctcggc ggtccagttc tcggcccacc cgtcggcgac
    10621 caccctgatg gcgcccttct tgatggcggg ctcgagcacc ttcatctggg cctcgcgcag
    10681 gatcttcgcg ttgtcgtcgg tgggtgagcc gccgatcagg atgtagttgc ccttgggcgc
    10741 cgcggacagg atgtgcgacg cctgcagcgt gccgatgcgc ggcacgtcga agccgacgta
    10801 gagggccagg tcggcgttct ggatcagccg gtcgtaggcg atcaccggca ccttctgcgc
    10861 gttggccttc tcgacgattt cggccgcctt ctcggtgtcg tgagccacga tgatgagcga
    10921 cttgacgccc tccgcaagca tcttcctggc ctgctggacc tgcctggcgt gatcggagtc
    10981 ggcgacctcc accagcaggt cgccgcccat gtcggtgacg atctcctgga tcatgtcgcg
    11041 gtcgcgcccc cagcgctcgt gcacgttgtc gagcaggagg ccgaccttca cgcgctcggg
    11101 gcccttttcc gcgggtggtg tgccgctccc gccaccacag ccggtgatgg cgagcgcgcc
    11161 gacgagtgac acgagagcga atgtgc
//
LOCUS       DAOPQX010000089        11182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6687_length_11182_cov_5.140918, whole genome shotgun sequence.
ACCESSION   DAOPQX010000089 DAOPQX010000000
VERSION     DAOPQX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6687_length_11182_cov_5.140918"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="PKK95_06765"
     CDS             complement(<1..2027)
                     /locus_tag="PKK95_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC17949.1"
                     /translation="MAIVSILAAAAPAAGQGREIAIKGATVLTVTKGTIPNGTVVVRD
                     GKIVAVGASVPIPPGAAVVDAAGRFLSPGLVDEHSHIASDSTNEGGTTVSSMTNVMDV
                     LNPADVDIYRDLAGGLTVASVFHGSANPIGGTNTVIKLRWGKSRAEDLVFDGALPGLK
                     FALGENPKDMRQFGQTGPRRYPASRPGVEFVIRDAFTRAKAYQRAWREYERRSQAGEA
                     GLAPRRDLQLEPLVEVLEGRRLAHVHAYRADEMLMMLRLAEEFGFRVATFEHGLEGYK
                     IAPELAAHGAGVGTFADWWGYKIEAIDAIPYNAALMMRAEVVVSINSDSAEHARRLNS
                     EAAKMMKWGGLTEEEALALVTINPARQLKVDRRVGSIEVGKDADLVLWSHHPLSSYAI
                     VERTYIDGTLYYERQADLARIEALSKEKAALIAAEGEGGAKADQGEAAKGAKAEVRKG
                     DAGKGGRGKGEAAAASGAADGPASIRPPAATADEGDLARLAPKMTAGSLAKGTLAITN
                     AKIVPVTRPAIERGTIVVRDGRIVALGESVDVPAGATVIDAAGAGVYPGWIDARTTVG
                     LSEPGPRGFEDTSEMLDYNPQLRTVVAYHNDSEAIPVARANGVATVAVFPAGGLLGGQ
                     VPVMNLDGWTWEESTLTPIAGISFQFPGVGARRGSYGAPPAPERPYEELRK"
     gene            complement(2192..2764)
                     /locus_tag="PKK95_06770"
     CDS             complement(2192..2764)
                     /locus_tag="PKK95_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOC17950.1"
                     /translation="MNVKRVVLMALVAVLLAAPAARADWFVSPLIGVNFGGDTNEDSR
                     KPPVGVSLGYMGGGIIGFETTFLYTSQFFNDEDLIGDNNVWSWMGNLIVGAPVGDEQQ
                     IRPYVSGGIGVLRSRIGGGANDLFDISRNDFGVNAGIGVMGFFTPNVGLRGDVRYFRS
                     VSDNDEGAGFDVDFGDFDFWQVTGGLAFRF"
     gene            complement(2900..4339)
                     /locus_tag="PKK95_06775"
     CDS             complement(2900..4339)
                     /locus_tag="PKK95_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HOC17951.1"
                     /translation="MSDKPFGDMDPETFRREGYRVVDWIARYFAEPERYPVMSRVRPG
                     EVRGALPAAAPERGEAFDAILQDFERVIVPGITHWNHPGFFAYFAISGSGPGVLAEML
                     SAALNAQGMLWRTSPSVTELEEVATSWLRDLMGLPAAFQGVIYDTASVSSLHALAAAR
                     EAAVPGVRREGMTGRSELPRYRVYCSEHAHSSIDKATILIGIGHDGLRKIPADGDFRM
                     RPDALEAAIAEDRRAGIVPLAVVATVGTTSTTSVDPVEAIADICAREGAWLHVDCAYA
                     GVAAIVPEYRHILRGVDRADSVVVNPHKWLFTPFDLSAFYTRRMDVLVGAFALTPEYL
                     RTSEAGAVRNLMDTGVQLGRRFRALKLWMILRYFGAEGIRERLGEHMRLARLFASWVD
                     EHPDFERLAPVPFSVVCFRSRPRGRGWTEDELEMRNQLLLDRLNATGEVFLSHTKLDG
                     RFVLRLAVGNLRTEERHVRRAWELVQQLA"
     gene            complement(4389..4940)
                     /locus_tag="PKK95_06780"
     CDS             complement(4389..4940)
                     /locus_tag="PKK95_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HOC17952.1"
                     /translation="MKKLAAALALILPLLALPSAAFPQVPASPPGIRNFTPVDSTVAC
                     AGAVTPEAAADVGKAGFVSIVNLRTAGEEGVDLSAIEKAAAQAGLRYFHVPFSSSNPT
                     PEPIDRFLEIARDRSNEPMLIYCASGGRAAMLLAIKRVMIDLWSVEKAMAEVPSLSEG
                     MRPAVREFGLAYLKQHGKAVPGK"
     gene            complement(5007..5729)
                     /locus_tag="PKK95_06785"
     CDS             complement(5007..5729)
                     /locus_tag="PKK95_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HOC17953.1"
                     /translation="MNPLRAGADLPLLPPPEMTSAPFAVMLAAAGILAAGAGMADPGP
                     FSPQAPDRPVRQRSVPANATPRPAVPDELAVPADAVLGLQLETLVTTPDVGEEEPVVA
                     RVIRDVMVGGRAAVPTGARVLGTATLVEKTPSGKEGPRLEVHFDTIELPGGERVEIDT
                     EPVTRDVGGRAPSLGRLGSGAAGGALVGAILGGGRGAAIGGALGAAGAAVTAPGRDRV
                     ELPAGTVLSVRTREEFRVRVRR"
     gene            5866..6603
                     /locus_tag="PKK95_06790"
     CDS             5866..6603
                     /locus_tag="PKK95_06790"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOC17954.1"
                     /translation="MKLADHFTRSGDFLFRWRGYLPLVMLPFFALGLLDAGLPQPVPA
                     FTRGVQVVAVAIALAGLAVRVVAIGTAAEGTSERSTVNPRASRLRTTGLYSLMRHPLY
                     AGNTVTAIGLAAFTTRWYLPVIVLLLGLLYHERIAAREEAFLEDRFGKAFREWADQVP
                     AMLPLVTRYRRADVPFGWRRPLGSEFHGLMTIASVVLVLDLFRSWLATGRLVFDALWT
                     GLFLAAAALFAGCTVLKKTTSVFEARG"
     assembly_gap    6751..6850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7175..7459)
                     /locus_tag="PKK95_06795"
     CDS             complement(7175..7459)
                     /locus_tag="PKK95_06795"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOC17955.1"
                     /translation="MLLPRDEIERRLPQLNGWRLEGNAIVRQFTFGGFPEAVAFVNRL
                     VPGCEAADHHPDITINYRRVTLSYSTHSEGGLTEKDFAGAAEADKLATGS"
     gene            complement(7468..8571)
                     /locus_tag="PKK95_06800"
     CDS             complement(7468..8571)
                     /locus_tag="PKK95_06800"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOC17956.1"
                     /translation="MFAAFKLPFSWWELIKRTAKDSIDDGITGLAAQLAYYFFLALFP
                     ALLFLVALASFFPLQQAIDQITGALVRFAPGDVVKIVQEQLAAISNSRNGGLLTIGFL
                     GTLWSTSSAMTGIIDTLNKAYEIEEGRPWWKVRLLAIGLTVAVGLFILVSFTLIVVGP
                     QLAERIAESAGLGGAFATTWKIAQWPLVFLLVAVAIGFIYYFGPDAEQQWEWITPGSL
                     VATLLWIGGSLGFRFYVTNFADYNETYGTIGGIIIALLWLYITGLSILVGAEMNAEIE
                     HASPYGKEAGEKRPGEKRKLGRLAARDRERRLREEEERRRREPSLPTVAPGMAAYPAA
                     LASDPRPGGRMSRWLLGAAVMGWSLFRGRKVRG"
     gene            8672..>11182
                     /locus_tag="PKK95_06805"
     CDS             8672..>11182
                     /locus_tag="PKK95_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOC17957.1"
                     /translation="MLSPRFHGPTAATAIALTLILSAPALGQGTRAPAQKIDAEYTAK
                     IKEYTQDPRVITELVDHLPASATVPTPLKFLGRIPGTPDELTYYKDILRYMEALEKAS
                     PRVKLLRIGKSEEGRDMIMVFVADEATITGLERYRKILNDLADPRTLTEAQARQLIAT
                     GKPIYWATGNLHSPETGSAEMLMELAYRLAVEETPFIRDIRNKVIVALTPVLEVDGRE
                     RVVDNFYFKKKTGQTLPLTWWGQYVAHDNNRDGLGVGLALTENVLRAFLHWKPTVLHD
                     LHESIPYLYVSTGAGPYNVNLDPVVTDEWWMLAKHEVSEMTKRGIPGVWTGGFYDGWT
                     PNYLFFIANLRNSIGRFYETQTYGPQNQEIKLGATQTSREWYRPNPPLPSIKWGPRNN
                     VNMQQSALLFALQYTARNRDLLLENFYLKGRRAVERGRSEAPHAWVIPASQHRRGEAA
                     ATVNMLRMQGVEVHRAGGAFKAGRVAAEPGDYVVRMDQPYGVLVKTLLGTQFFPPQNP
                     SPYDDTGWSVPLMRNLAVHRVDDKSILDLPATLLTADVRPEGGISGSGRVLIVEHNAD
                     NALVTFRFALPAVRMLAAEAPFEAAGRKFEAGAFLVPDADPAVVLPVLKELGLTAHAV
                     AAAPEVKTHELDVPRIGYVHSWQRTQDEGWVRMAFDKFKVPYTYFGDTRLREGNLRAK
                     YDVIVFPHVGGTPQSQVNGLPMTGDPLPHKKSELTPNLGVQDSADDIRGGMGLEGLAN
                     LAAFVREGGLLITEGSTATIFPAYGVTQGVSVDEAPGLFARGAILKAVFADRKSPIAY
                     GYNADRLPVYFNQAPVLSVGVGGFGRGGQPIPGVGMNLT"
BASE COUNT         1736 a         3923 c         3736 g         1687 t
ORIGIN      
        1 tccttgcgca actcctcgta ggggcgctcc ggcgcgggcg gcgctccgta cgacccgcgc
       61 cttgctccga cgccgggaaa ctggaagctg atgcccgcga tcggcgtcag ggtgctctcc
      121 tcccaggtcc acccgtccag gttcatcacc ggcacctggc ccccgagcag cccgccggcc
      181 gggaacactg ccacggtcgc cacgccgttg gcacgcgcaa ccgggatcgc ctcgctgtcg
      241 ttgtggtagg cgaccaccgt tcgcagctgc gggttgtagt cgagcatctc ggaggtgtcc
      301 tcgaaccccc ggggccccgg ctccgacagg ccaaccgtcg tccgcgcgtc gatccagcct
      361 gggtagaccc cggccccggc cgcgtcgatc accgtcgccc ccgccgggac gtcaaccgat
      421 tcgcccagcg ccacgatgcg gccgtcgcgg acgacgatgg tcccgcgctc gatcgccggg
      481 cgggtcaccg gaacgatctt cgcgttggtg atggccagcg tccccttggc gagggacccg
      541 gccgtcatct tcggcgccag ccgcgccagg tcgccctcgt ccgccgttgc ggccggcggc
      601 ctgatcgacg ccgggccgtc cgcggcgccg ctcgccgcgg ccgcctcacc cttgccccgc
      661 ccacccttgc ctgcatcgcc cttgcggacc tcggccttgg cacccttggc cgcttcgccc
      721 tggtctgcct tcgcgccgcc ctcgccctcg gccgcgatca gcgccgcctt ctccttcgag
      781 agcgcctcga tgcgcgcgag atcggcctgg cgctcgtaat acaaagtccc gtcgatgtag
      841 gtccgctcga cgatcgcgta cgagctgagc gggtgatgcg accagagaac gaggtcggcg
      901 tccttgccaa cctcgatgga gcccacgcgc cggtccacct tcagctgccg ggccgggttg
      961 atggtgacca gcgcgagggc ctcctcctcg gtcagcccac cccacttcat catcttcgcg
     1021 gcctcgctgt tgagccgccg cgcatgttcg gcgctgtcgg agttgatcga caccaccacc
     1081 tccgcgcgca tcatcagcgc cgcgttgtac gggatggcgt cgatcgcctc gatcttgtac
     1141 ccccaccagt cggcaaaggt accgacgccc gcaccatgcg ccgccagctc gggcgcaatc
     1201 ttgtagccct cgagaccgtg ctcgaaggtg gcgaccctga agccgaactc ctcggccagg
     1261 cgcagcatca tcagcatctc gtcggcgcgg tacgcgtgca cgtgcgccag ccgccgcccc
     1321 tcgagcacct ccaccagcgg ctcgagctgc aggtcgcggc ggggcgcgag gccggcctcg
     1381 ccggcctggg atcgccgctc gtactcccgc caggcgcgct ggtaggcctt ggcacgcgtg
     1441 aacgcgtcac ggatcacgaa ctcgacaccc gggcggctcg ccgggtaacg ccgcggcccg
     1501 gtctgcccga actgccgcat gtctttcggg ttctcgccca gggcgaactt cagccctggc
     1561 agcgccccgt cgaacaccag atcctcggcc cgcgacttgc cccaccgcag cttgatgacg
     1621 gtgttggttc cgccaatcgg gttcgcgctg ccgtggaaca cgctcgcgac ggtcaggccg
     1681 cccgcgaggt cccggtagat gtcgacgtca gccgggttga gcacgtccat cacgttggtc
     1741 atggagctga ccgtcgtccc cccctcgttg gtggagtccg atgcgatgtg cgagtgctcg
     1801 tcgacgaggc ccggactgag gaaccgcccg gctgcatcga ccaccgcggc cccgggcggg
     1861 atcgggacgc ttgcgccgac ggcgacgatc ttcccgtcgc gaacgacgac ggtgccgttg
     1921 ggaatcgtgc ccttggtgac cgtgagcacc gtggcgccct tgatggcgat ctcgcggcct
     1981 tgcccggcgg ccggggcggc cgccgccaga atcgacacga tcgccatcgc gcctaaagtg
     2041 cagcgaagca tggtctgcct ccgagggtgg gttccaggtc gtgctggggg cgccggggcg
     2101 ggagaagtat agtgcaggaa gggagcacga cgcgccggga gcgtgtccga gccaaccgcg
     2161 cggacacgct cctcacgcaa cgccaggcgg cctagaagcg gaaggcgaga ccgccggtca
     2221 cctgccagaa gtcgaagtcg ccgaagtcca cgtcgaaccc ggccccttcg tcattgtccg
     2281 aaacgctccg gaagtaacgc acgtcgccgc gcaggcccac gttgggggtg aaaaagccca
     2341 tgacgcctat gcccgcgttg acgccgaagt cgttgcgcga gatgtcgaac aggtcgttcg
     2401 cgccaccgcc aatccgggag cgcagcacgc cgatgccgcc cgagacgtac ggacggatct
     2461 gctgttcgtc accaaccggc gcgccgacga tcaggttgcc catccagctc cacacgttgt
     2521 tgtccccgat cagatcctcg tcgttgaaga actgggacgt gtagaggaag gtggtctcga
     2581 acccgatgat gccgccgccc atgtagccga gcgagacgcc gaccgggggc ttgcggctgt
     2641 cctcgttggt gtcgccaccg aagttcacgc cgatgagcgg cgaaacgaac caatcggccc
     2701 tggcagccgg ggcggccagc aacaccgcca ccaaggccat cagcacaact ctcttgacgt
     2761 tcatacctcc tcaactcctt ccagttttct tcagataccc gccgcattgc attgcaatcg
     2821 acgtgcccca gaagggtcga gccgtctgtg aaaggcagag gtgcccactg ccgacggtat
     2881 tcagttggca gcggtggcgt caggcgagct gttggaccag ttcccaggcc cggcggacgt
     2941 gccgttcctc cgtgcgcagg ttgcccacgg ctagacgcag cacgaaccgg ccgtcgagct
     3001 tggtgtgcga gaggaacacc tctccggtgg cattcaggcg atcgagcagc agctggttcc
     3061 gcatctccag ctcgtcctcg gtccaccccc tgccccgggg cctcgagcgg aagcagacaa
     3121 cgctgaacgg aacgggggcg agacgctcga agtcgggatg ttcgtcgacc catgaggcga
     3181 acagccgcgc cagccgcatg tgctcgccaa gccgttcccg aatcccctcg gctccaaaat
     3241 accggaggat catccacaac ttcagagcgc ggaatcggcg gcccagctgc acgcccgtat
     3301 ccatgaggtt gcgcaccgcg ccagcctcgc tggtccgaag gtactcgggc gtcagcgcga
     3361 acgcgccgac caggacgtcc atgcgccgcg tgtagaacgc gctcaggtcg aacggggtga
     3421 agagccactt gtgcgggttc accaccaccg agtcggcgcg gtcgacgccg cgcaggatgt
     3481 gccggtactc ggggacgatc gcggccaccc cggcgtacgc gcagtccacg tgcagccacg
     3541 ccccctcccg cgcgcagatg tcggcaatcg cctccaccgg gtcgacgctc gtcgtggagg
     3601 tggtgcccac cgtggcgacg acggcgagcg ggacgatgcc ggcccgccgg tcttccgcga
     3661 tcgccgcctc gagcgcgtcg gggcgcatcc ggaagtctcc gtcggcgggg atcttccgca
     3721 acccgtcgtg gccaatcccg atcaggatcg tcgccttgtc gatggaggaa tgcgcgtgct
     3781 ccgagcagta cacccggtag cgcggcaatt cggaccgccc ggtcatcccc tctcgccgca
     3841 cgccgggcac cgccgcctcg cgcgcggcgg ccagggcatg caggctcgac accgaagcgg
     3901 tgtcgtagat gacgccctgg aaggccgccg gcagccccat caggtcgcgc agccacgagg
     3961 ttgccacctc ttccagttcg gtgaccgagg gcgaggtccg ccagagcatc ccctgcgcgt
     4021 tcagggccgc cgacagcatc tccgccagca cgcccgggcc gctccccgag atggcgaaat
     4081 aggcgaagaa cccggggtgg ttccagtgcg tgatgccggg aacgatcacg cgttcgaagt
     4141 cctgcaggat ggcgtcgaac gcctcgcccc gctcgggcgc cgcggcgggc agcgcgccgc
     4201 gcacctcgcc cggcctcacg cgcgacatca ccgggtagcg ctcgggctcg gcgaagtacc
     4261 gcgcaatcca gtccacgacc cggtacccct cgcgcctgaa cgtctcgggg tccatgtcac
     4321 cgaacggctt gtcggacacg ggcaacctcg aaggagaaaa gggggtcgtg gagaaccccg
     4381 ccgacagcct acttcccggg gactgccttg ccgtgctgtt tcagatacgc caacccgaac
     4441 tcgcggacag ccggccgcat accctcgctc aggctcggca cctcggccat cgccttctcc
     4501 acgctccaga ggtcgatcat gacgcgcttg atcgcgagca gcatcgcggc acggccgccg
     4561 ctggcgcaat agatgagcat cggctcgttc gacctgtcgc gggcgatctc gaggaagcgg
     4621 tcgatcggct cgggcgtcgg gttggacgag gagaaaggca cgtggaagta gcgcaggccg
     4681 gcctgggcgg ccgccttctc gatcgccgag aggtcaaccc cctcctcgcc ggcggtgcga
     4741 aggttgacga tcgacacgaa gcccgccttg ccgacatcgg ctgcagcctc gggcgtcacc
     4801 gccccggcgc acgcgacagt ggagtcgaca ggggtgaagt tgcggatgcc tggcggcgag
     4861 gccggcacct gcgggaaggc ggcggaaggc agggcgagga gcgggaggat cagggccagg
     4921 gctgcggcaa gtttcttcat tcctccgtca ttctaatcct ggtgccttcg ccccgccgga
     4981 ttcgcccgag cggcccgcta actgggctat cgcctcaccc tcacccggaa ctcctccctg
     5041 gtgcgcacgc tcaacaccgt tccggctggc agttcgaccc gatcgcgccc tggagcggtg
     5101 acggcggcac ccgccgcccc aagcgctccc ccgatggcgg ctccccggcc gccgccgagg
     5161 atcgctccca ccagcgcacc gccggcggcc ccgctgccga gccggccgag gctcggcgca
     5221 cggccgccca cgtcgcgcgt cacgggttcg gtgtcgatct ccacgcgctc gccgcctggc
     5281 agctcgatcg tgtcgaaatg cacctccagg cgaggcccct ccttgcccga gggcgtcttc
     5341 tcgaccagcg tcgcggtgcc cagcaccctt gcccccgtgg gtaccgccgc gcgtccgccg
     5401 accatcacat cgcggatgac ccgggccacc accggctctt cctcgcccac gtccggcgtg
     5461 gtgacgagcg tctcgagctg gagaccgaga acggcgtcgg caggcacggc aagctcgtct
     5521 ggaacggcag gacgcggcgt cgcgttggcc ggcactgacc gctggcggac cggccggtcg
     5581 ggcgcctgcg gggagaacgg gccgggatcg gccatcccgg cccctgcggc caggattccc
     5641 gcggcagcta gcatgacggc gaatggggcg ctggtcatct ctggcggcgg cagcagcggc
     5701 aggtcggcac ccgcccgaag gggattcaag aggcgtaccc ccaggaacag gccagatttg
     5761 tgggggtttc ctccggccgg accctggaac gtcctctccg gttgtcgccc ggcggggtcg
     5821 gtttcgaggt gcggcgtgcc ggggatgccg cataatgggc gaccgatgaa gctggccgac
     5881 cacttcaccc gatcgggtga cttcctcttc cgctggcggg gctacctgcc gctggtgatg
     5941 ctgccgttct tcgccctcgg tcttctcgac gccgggctgc cgcagccggt gcccgccttc
     6001 acccgcggtg tacaggtcgt cgccgtggcg atcgcgctgg ccggcctagc cgtccgcgtg
     6061 gtcgccatag gcaccgccgc cgagggaacc tccgagcgaa gcacggtcaa cccgcgagcc
     6121 tcgcggcttc gcacgaccgg actctactcg ctgatgcgcc acccgctcta cgcgggcaac
     6181 acggtcacag cgatcggcct ggccgccttc acgacgcgtt ggtacctgcc ggtcatcgtc
     6241 ctgctgctgg ggctgctcta tcacgagcgg atcgcggccc gggaggaggc gttcctcgag
     6301 gatcggttcg gcaaggcgtt cagggagtgg gccgaccagg tgccggcgat gcttccgctg
     6361 gtcacacgct accgccgcgc ggacgtcccg ttcggctggc gccgcccgct cggctccgag
     6421 ttccacggcc tgatgaccat cgcttcggtg gtgttggtcc tcgacctctt ccgtagctgg
     6481 ctggccacgg ggcgcctggt gttcgacgcc ctctggaccg ggctgttcct ggccgccgcg
     6541 gccctcttcg ccggctgcac ggtgctgaag aagacgacga gcgtgttcga ggcgcgaggg
     6601 tagcggggta gcggatcaga tcgtgccgga ggtgcagagg gtgcaggagg tgcaggaggt
     6661 gcagtgccga tggtgctgga ggtgctagag gtgcagtgcc gatggtgctg gaggtgctgg
     6721 aggtgccgtg ccgatggtgc tggaggtgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn ggtgctggag gtgctggagg tgccgtgccg atggtgctgg aggtgctgga
     6901 ggtgcagtgc cgatggtgct ggaggcgcag tgctgatggt gccggaagtg ctggtgccga
     6961 gagtgccgct tgctgacgat cccctacacc cgcacctttg gcaccaccag cactagcacc
     7021 cgcggcacct cctgcacttc cagcacttcc agcacgacca cgtgaatgat cagcgatctc
     7081 tgcgtacgcc tccgcgtctc cgtgtctctg cggcctgccg cgccgtagcg caaagcgcga
     7141 aggcgggttg aacgtggaca ggccgccacg tctgtcagga gcccgtcgcg agcttgtcgg
     7201 cctcggcggc gcctgcgaag tccttctcgg tgagcccgcc ctcgctgtgc gtcgagtagg
     7261 acagcgtcac gcggcggtag ttgatggtga tgtcggggtg gtgatcggcc gcttcacagc
     7321 ccggcaccag gcggttgacg aaggccacgg cctccgggaa gcccccgaag gtgaactgcc
     7381 ggacgatggc gttgccctcg aggcgccacc cgttcagctg tggcagccgg cgctcgatct
     7441 cgtctcttgg aagcagcatc gaaactccta gcccctgacc ttccttcccc ggaacagcga
     7501 ccaccccatg accgcggcgc caagcagcca gcgcgacatc ctcccgcccg gccgcgggtc
     7561 cgacgcgaga gccgcggggt aggcggccat accgggggcg accgtgggga gcgagggctc
     7621 acggcgccgc cgctcctctt cctcgcgcag ccgccgctcg cggtccctgg cggccagccg
     7681 tcccagcttc ctcttctcgc cgggcctctt ctcgccggcc tccttgccgt acggcgaggc
     7741 gtgctcgatc tcggcgttca tctcggcgcc cacgaggatc gacagtccgg tgatgtagag
     7801 ccagaggagc gcgatgatga tcccgccgat cgtgccatac gtctcgttgt agtcggcgaa
     7861 gttggtgacg tagaagcgga agccgagcga cccgccaatc cagagcagtg tcgccaccag
     7921 cgagcccggc gtgatccact cccactgctg ctcggcgtcc ggcccgaagt agtagatgaa
     7981 gccgatggcg accgccacca gcaggaagac gagcggccac tgcgcgatct tccaggtcgt
     8041 cgcgaaggcg ccgccgaggc ccgcgctctc ggcaatccgc tccgccagct ggggaccgac
     8101 cacgatgagg gtgaaggaga cgaggatgaa gaggcccacc gcgacggtga ggccgatcgc
     8161 cagcaggcgg accttccacc aggggcgccc ctcctcgatc tcgtaggcct tgttcagggt
     8221 gtcgatgatg ccggtcatcg cgctcgaggt ggaccacagc gtgccgagga agccgatcgt
     8281 cagcaggccg ccgttccggc tgttcgagat ggcggccagc tgctcctgca cgatcttgac
     8341 gacgtcgccc ggggcgaacc ggacgagggc cccggtgatc tggtcgatgg cctgctggag
     8401 cgggaagaag ctggcgaggg ccacgaggaa cagcagggcc gggaacagcg ccaggaagaa
     8461 gtagtaggcg agctgcgccg cgaggcctgt gatcccgtcg tcgatcgagt ccttcgccgt
     8521 gcgcttgatc agctcccacc aggagaacgg cagcttgaag gccgcgaaca tgccaccggt
     8581 tcgtgcaaca gggctgccag cgaggatctg tgctataacc cgccgtgccg ccggcacgga
     8641 ggccggcgcc accgctggca ggagatccgc gatgttgtca ccgaggttcc acggcccgac
     8701 cgccgccacc gcgattgccc tcaccctgat cctctcggct cccgcgctcg gccaggggac
     8761 cagggccccg gcgcagaaga tcgacgccga gtacaccgcg aagatcaagg agtacaccca
     8821 ggacccgcgg gtcatcaccg agctggtcga tcacctgccg gcgtcggcaa cggtgccgac
     8881 gccgctgaag ttcctcggcc ggatcccggg tacaccggac gagctgacgt actacaagga
     8941 catcctccgc tacatggagg cgctcgagaa ggcgtcgccg cgcgtgaagc tgctgcgcat
     9001 cggcaagagc gaggaaggcc gcgacatgat catggtcttc gtcgccgacg aggcgaccat
     9061 caccggcctc gagcgctacc ggaagatcct caacgacctc gccgatccca ggacgctcac
     9121 cgaggcgcag gcgcggcagc tcatcgcgac cggcaagccc atctactggg cgactggcaa
     9181 cctccactcc ccggagaccg gcagcgccga gatgctgatg gagctggcct accgcctggc
     9241 cgtcgaggag acgccgttca tccgcgacat ccgcaacaag gtgatcgtcg ccctgacgcc
     9301 ggtgctcgag gtggacgggc gcgagagggt ggtcgacaac ttctacttca agaagaagac
     9361 ggggcagacc ctgccgctga cgtggtgggg ccagtacgtc gcgcacgaca acaaccgcga
     9421 cggtctcggg gtcggcctcg cgctgaccga gaacgtgctg cgggccttcc tccactggaa
     9481 gccgaccgtg ctgcacgacc tccacgagtc gatcccctac ctctacgtct cgaccggggc
     9541 cgggccctac aacgtgaacc tcgacccggt ggtcaccgac gagtggtgga tgctcgccaa
     9601 gcacgaggtc agcgagatga ccaagcgcgg catccccggc gtctggacgg gcggcttcta
     9661 cgacggctgg acgccgaact acctgttctt catcgcgaac ctgcgcaaca gcatcggccg
     9721 cttctacgag acgcagacct acgggccgca gaaccaggag atcaagctgg gcgcgacgca
     9781 gacgagccgc gagtggtacc ggccgaaccc gccgctcccg tcgatcaagt ggggccctcg
     9841 caacaacgtg aacatgcagc agagcgccct cctgttcgcg ctgcagtaca cggcacgcaa
     9901 ccgcgacctc ctgctcgaga acttctacct caagggccgg cgcgccgtgg agcgcggccg
     9961 aagcgaagcc ccgcacgcct gggtcatccc cgccagccag caccggcgcg gcgaggcggc
    10021 ggccaccgtg aacatgctcc ggatgcaggg cgtcgaggtg caccgggctg gcggcgcgtt
    10081 caaggccggc cgcgtggcgg cggagccggg cgactacgtg gtccgcatgg accagcccta
    10141 cggcgtgctg gtgaagacgc tgctcggcac gcagttcttc ccgccgcaga acccgtcgcc
    10201 ctacgacgac accggctgga gcgtccccct catgcggaac ctggccgtcc accgggtgga
    10261 cgacaagtcg atcctcgacc tgccggcgac gctgctgacg gcggacgtcc ggcccgaggg
    10321 gggcatctcg ggctcggggc gcgtgctgat cgtcgagcac aacgccgaca acgcgctcgt
    10381 gaccttccgc ttcgccctgc cggcggtgag gatgctggcg gccgaggcac ccttcgaggc
    10441 ggccgggcgg aagttcgagg cgggcgcctt cctcgtcccc gacgccgacc cggctgtcgt
    10501 cttgcccgtg ctgaaagagc tggggctcac cgcccacgcg gtggccgcgg cgcccgaggt
    10561 caagacgcac gagctcgacg tgccccgcat cggctacgtc cactcctggc agcggacgca
    10621 ggacgagggg tgggtgcgga tggcgttcga caagttcaag gtgccctaca cctacttcgg
    10681 cgacaccagg ctgcgcgagg gaaacctgcg cgcgaagtac gacgtgatcg tcttccctca
    10741 cgtaggcggt accccgcagt cgcaggtgaa cggcctgccg atgaccgggg atcccctgcc
    10801 gcacaagaag tcggagctga cgcccaacct gggcgtgcag gactcggctg acgacatccg
    10861 cggcggcatg ggtctcgagg ggctggccaa cctggcggcg ttcgtccgcg agggcgggct
    10921 gctgatcacc gaaggatcga cggcgacgat cttccccgcc tacggcgtca cgcagggcgt
    10981 cagcgtagac gaggcgcctg gcctgttcgc gcggggggct attctcaagg cggtatttgc
    11041 cgatcgcaag agcccgatcg cttacgggta caatgccgat cgcctgccgg tgtacttcaa
    11101 ccaggcgccg gtgctctcgg tgggcgtggg cggcttcggc cggggcggcc agcccattcc
    11161 cggcgtcggc atgaacctca cg
//
LOCUS       DAOPQX010000090        11044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_6778_length_11044_cov_4.039676, whole genome shotgun sequence.
ACCESSION   DAOPQX010000090 DAOPQX010000000
VERSION     DAOPQX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11044
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6778_length_11044_cov_4.039676"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKK95_06810"
     CDS             complement(<1..833)
                     /locus_tag="PKK95_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOC17958.1"
                     /translation="MTPGESPGGAGDAVAQALAEARARMADADRKILRLIDERLELAL
                     RAGRLEEAVPSSRREPAEPATPPAPGGLLTNDFVAGLFRAIAGEGERIRKDGRGLAGF
                     QGERGAWSEMACLAWDARFVPIPCRGFADVFDGVARGLLDRGVVPVENSLGGEVADVS
                     DLLVARNVSIVGEVVVPVAQCLLALPGTRLGSLRTVYSHPQALVQCRAFIEAHGLEAQ
                     PFYDTAGAARWLMFEGVRTAGVIASPLAATLYGLEVVAEGIADEPANETRFVVISNTP
                     EA"
     gene            complement(830..1843)
                     /gene="ilvC"
                     /locus_tag="PKK95_06815"
     CDS             complement(830..1843)
                     /gene="ilvC"
                     /locus_tag="PKK95_06815"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOC17959.1"
                     /translation="MARMYYDSDADLSLVTSRKVAIIGYGSQGHAHALNLRDSGVTVR
                     VGLPPGSASRAKAEAEGLEVTSPADAARWADLTMVLAPDTAQPAIYERDIAPHLAPGR
                     TLMFAHGFNIRYGTIAPPGEVDVALVAPKSPGHRVREVFREGGGTPALLAVHQDASGQ
                     ARQVALAYAKGIGATRAGVIETTFAEETETDLFGEQAVLCGGVSALVKAGFETLVEAG
                     YQPEVAYFECLHELKLIVDLIYRGGLSYMRYSVSDTAEHGDYTAGPRIVSEQVRQEMR
                     RLIEEIRDGSYAKAWIQENAAGRPWFEQARRAERCHQIEEVGRGLRAMMPFIEPVGIP
                     EGR"
     gene            complement(1856..2359)
                     /gene="ilvN"
                     /locus_tag="PKK95_06820"
     CDS             complement(1856..2359)
                     /gene="ilvN"
                     /locus_tag="PKK95_06820"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOC17960.1"
                     /translation="MTRILVVLARDGPDVLMRIASLCRRRAFAVESLTIGRTEAPGVS
                     RVTLFVATDPAGARRLAANLAKLVDVLEVEDVSDLPIVVRDLAMIKVAAPPDARAGIM
                     QLVQVFRARVVDVAADSLVIETTGTDDKVDGLLQVLRPFGVIEVARSGRLAMCRGPRA
                     AAPAGAA"
     gene            complement(2363..4132)
                     /gene="ilvB"
                     /locus_tag="PKK95_06825"
     CDS             complement(2363..4132)
                     /gene="ilvB"
                     /locus_tag="PKK95_06825"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOC17961.1"
                     /translation="MPKISFPGQAARRPEPSLTGAQAVWETLARLGTTHVFGYPGGAI
                     LPIYDALPHAGISHVLVRHEQAAAHMADGFARATGRVGVAMATSGPGATNLVTGIATA
                     MMDGSPIVCITGQVGSSQLGLDAFQEVDITGITMPITKHNYLVTDASAIAPAIAEAFH
                     VASSGRPGPVLVDITKDAQQSRCASEEYAPVARRPRRPAAPPLDMAACRAAAALIASA
                     RRPVILAGHGAIAPGSSRPLRALAERAGIPVASTLLGLGALPASHPLNLGLAGMHGDA
                     WVNQAVQEADLVVSLGARFDDRAVGNARGFAPKARRVHVDIDASEIGKVVEVDVGIEG
                     DLARVLPALLTLVEPGSRDEWLGEIRAARRAAGARDILNLPGDGRLHGAHIVSALWKH
                     TRGKALVVTDVGQHQMWAAQYYRPERSCSFITSGGLGTMGFGLPAAIGAQVARPGDEV
                     WVIAGDGGFQMTACELSTVVQERLPLRIAVINNAYLGMVRQWQELFYGKRYTATPMAS
                     PDFCKLAEAHGIPAHLVETPAEVSPAIAAARRQAGPVLVEFKVEADDLVYPMVPSGAS
                     LHEMLRRPQPRPRAVPSPAGKGA"
     gene            complement(4140..5819)
                     /gene="ilvD"
                     /locus_tag="PKK95_06830"
     CDS             complement(4140..5819)
                     /gene="ilvD"
                     /locus_tag="PKK95_06830"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOC17962.1"
                     /translation="MPPDGPMRAQSAPTLDGPSRAPARAMFKAIGLTDADLARPLVGI
                     ANTWTEIGPCNYHLRELAGHVKEGIRRAGGTPLEFGTIAVSDGISMGTEGMRASLVSR
                     EVIADSIELVARAHGLDAVVALVGCDKTIPGAVLALARLDLPGLVLYGGAIAPGRAGG
                     CDLTIQDVFEAVGAHAAGRLDDEGLREIEDAACPGPGACGGQFTANTMALACEFLGIS
                     PMGSASVPAADAGRADVAARAGALVMQLLERGLRPKDVITRASIENAIAGVAATGGST
                     NAVLHLLAIAREAGVALGIDDFDRISGATPLLADLKPGGRFTVSDLYRAGGSALVAAR
                     LLEAGRLHAGCATVSGLTIGEAAAGAREADGQQVVRPLDRPISPSGGLVILKGNLAPD
                     GCVVKVAGYTRRQHEGPARVFESEEAAFEAVQGGGIAPGTVIVIRNEGPRGGPGMREM
                     LAVTAAVNGAGLGDSVALVTDGRFSGATRGLMAGHVSPEAACLGPIAAVRDGDVVRFD
                     IGQRLLEVELTPEQVAERLAAWRQPPPRYRAGVLNRYARAVSSAALGAVLE"
     gene            6459..7544
                     /locus_tag="PKK95_06835"
     CDS             6459..7544
                     /locus_tag="PKK95_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOC17963.1"
                     /translation="MGLCLSGGGYRAMLFHLGALWRLNEAALLPGLDRISSVSAGSIA
                     AGVLGLRWLELEFRHGVASRFDELVAAPLRALAGWTIDEGAILSGALLPGAIAERLEA
                     ALRDHLFGDATLQDLPRDSEGPRFVINATSVQTGSLWRFSRPFMADWRVGRFLEPRVP
                     LAFAVAASSAFPPVLSPARLRLVPGIYTGVEPAELFGPPFDSEAVLTDGGVYDNLGLE
                     TVWKRYSTVLVSDGGGRLEPEGDPKRDWARHALRVHEIVDNQVRSLRKRQLIGSFRAG
                     YRRGTYWSIRCDPADYGVPSLASGEVTAGLAGVKTRLEAMDERLQERLVNWGYAACDV
                     ALRAHVRPGLPLPASFPYPGSPLDGRP"
     gene            7689..8837
                     /locus_tag="PKK95_06840"
     CDS             7689..8837
                     /locus_tag="PKK95_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOC17964.1"
                     /translation="MTSARRVGGAVLGRLLAAAMMVILPALPAPGQEAAVPKPSAPPV
                     ELPGTERLSLRSRVTGQEYALHVSLPRGYDKTGERFPVIYAVDGQWDFTLVHAIYGQQ
                     YYDGFLPGAIIVGITWGGEKPDYDRRRAFDLTPTSGGQPDRYGNAPRFLEFIEKEAIP
                     FVEARYRAARDGRTLTGSSFGGLFTLYALFTRPALFDRYVLTSPAWSWDNQVLLRHGE
                     QFARSPLPKPVKVFMGVGEYEDVAGFEKLAATIRGYGLAGLDLETRVIAGAGHSGAKT
                     EGFTRGLQFVFARPCARVPPEALSRLAGEYELGPAPAAVVRIAVEDGGLVAYPPSGPK
                     VRLCADSDLDFRLVGQYLKVHFIQAADKTVGSFRLEQYDGEAVAKRRK"
     gene            8875..9474
                     /locus_tag="PKK95_06845"
     CDS             8875..9474
                     /locus_tag="PKK95_06845"
                     /inference="COORDINATES: protein motif:HMM:NF002099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HOC17965.1"
                     /translation="MPTSMDVLPRAQRPAWTVRWWLAIAGLLLAQAAILLAMGRLPVC
                     ECGYVKVWHGVVHSSGNSQHVSDWYTFSHLIHGFLFYWVLSLALPRWKAGWRAVLALG
                     IEVAWEVIENTPFVIDRYRAATISLDYYGDTVLNSVSDSIACLAGFAAARKLPVAATI
                     LLALAFEAFTLYWIRDNLTLNVIMLLWPLDAVRAWQAGG"
     gene            9509..10402
                     /locus_tag="PKK95_06850"
     CDS             9509..10402
                     /locus_tag="PKK95_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOC17966.1"
                     /translation="MRHALAPATLALAAVLLLGAAARQQPAAPEADRGARPVKIYVNT
                     DLEGISGVFKFAQTREKDSPANIQACEYFMGDVAAVVRGLRDGGATEVVVLDGHGSQA
                     VIPHLMAPGAVYITGTPRPGAGNLSELDSSFAGMVMLGFHAMMGTPDGVLNHTQSSRT
                     ENRYWYNGVESGELVQNAAIAGHYGVPPILVTGDEATCREAKRFLGDDIVTVAVKKGL
                     ARESAVLYPFEETRKALYEGARRAMTTIGSRRPYVLKTPIAVKEQFLDLEPSLPKPRL
                     VTREGVARDALHLLSWAKEPR"
     gene            10615..>11044
                     /locus_tag="PKK95_06855"
     CDS             10615..>11044
                     /locus_tag="PKK95_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17967.1"
                     /translation="MTEWGVQLAMVRLADAIRQLGDGDRERQVRAAFLHVVERLQEGA
                     ALVTRDWDVGARHQDAPLWTLTDGASRTRAVLRFVEDYGFEVRVFVDDELRQARMFRH
                     DREAALQFARTRRAGLETRGWREWTVVPGSPEAGGDRAVSL"
BASE COUNT         1500 a         4024 c         3936 g         1584 t
ORIGIN      
        1 gcctccggcg tgttcgagat cacgacgaag cgcgtctcgt tggccggttc gtccgcgatc
       61 ccctcggcga cgacctcgag gccgtacagc gtggcggcga gcgggctggc gatgaccccg
      121 gccgtgcgca ccccctcgaa catcagccac cgcgcggccc ccgccgtgtc gtagaacggc
      181 tgtgcctcga ggccgtgcgc ctcgatgaac gcccggcatt gcacgagcgc ctgcgggtgc
      241 gagtagaccg ttcggagcga gcccagccgc gtgcccggca gggcgagcag gcactgcgcc
      301 accggcacga ccacctctcc gacaatcgag acgttgcgcg cgacgaggag gtccgagacg
      361 tcggccacct cgccgccgag cgagttctcc acgggcacga ccccgcggtc gaggaggccg
      421 cgcgcgaccc cgtcgaacac gtcggcaaac ccgcggcacg ggatcgggac gaaccgcgcg
      481 tcccacgcca ggcacgccat ctcgctccac gcgccccgct ctccctggaa accggccagg
      541 ccacggccgt ccttccggat ccgctcgccc tcgccggcga tcgcgcggaa gagaccggcc
      601 acgaagtcgt tggtcagcag cccgcccggc gcgggcggcg tggcgggttc cgccggctcg
      661 cggcgggatg aggggaccgc ctcctcgagg cggccggcgc gaagcgccag ctcgaggcgc
      721 tcgtcgatca ggcgcaggat cttcctgtcg gcgtcggcca tccgggcgcg agcctcggcg
      781 agcgcctggg cgacggcgtc accggcaccg ccaggcgatt cgccaggcgt caccgcccct
      841 ccgggatccc gaccggttcg atgaacggca tcatcgcccg cagccccctg ccgacctcct
      901 cgatctggtg gcaccgctcc gcgcgccggg cctgctcgaa ccaggggcgg ccggccgcgt
      961 tctcctggat ccaggccttc gcgtagctgc cgtcgcggat ctcctcgatc agccgccgca
     1021 tctcctggcg cacctgctcg ctcacgatcc tcggccctgc ggtgtagtcg ccgtgctcgg
     1081 cggtgtcgct caccgagtag cgcatgtagc tcaagccccc gcgatagatg aggtccacga
     1141 tcagcttcag ctcgtgcagg cactcgaagt aggcgacctc gggctggtag ccggcctcca
     1201 cgagcgtctc gaaccccgcc ttcaccagcg cgctgacccc gccgcacagc accgcctgct
     1261 cgccgaacag gtcggtctcg gtctcctcgg cgaaggtcgt ctcgatcacg ccggcgcgcg
     1321 tggcgccgat ccccttcgcg taggcgagcg ccacctgccg cgcctgcccg ctggcgtcct
     1381 ggtgcacggc cagcagggcg ggcgttccgc ccccttcccg gaagacctcg cgcacccggt
     1441 ggcccggcga cttgggcgcc accagcgcca cgtccacctc tcccgggggt gcgatcgtac
     1501 cgtacctgat gttgaagccg tgcgcgaaca tgagggtgcg ccccggcgcg aggtgcggcg
     1561 cgatgtcacg ctcgtagatt gccggctgcg ccgtgtcggg cgcgagcacc atcgtgagat
     1621 cggcccatcg tgccgcgtcg gccggggagg tgacctccag cccctcggcc tcggccttcg
     1681 cgcgcgacgc gctccccggc ggcaggccaa cccggacggt gacgccgctg tccctcaggt
     1741 tgagggcgtg cgcgtgaccc tgcgatccgt acccgatgat cgccaccttc cggctggtga
     1801 cgagagagag gtcggcgtcg ctgtcgtagt acatcctcgc cattgattcc tcgcttcaag
     1861 cggcccctgc cggcgccgcg gcccgcggcc cccggcacat cgccagccgg cccgaccgcg
     1921 ccacctcgat cacgccgaac ggccgcagca cctgcagcag cccgtccacc ttgtcgtcgg
     1981 tgcccgtcgt ctcgatcacg agcgagtcgg ccgcgacgtc cacgacgcgg gcgcgaaaca
     2041 cctgcaccag ctgcatgatg ccggcgcggg cgtcgggcgg cgcggcaacc ttgatcatcg
     2101 ccaggtcgcg gacgacgatc ggaaggtcgg agacatcttc cacctcgagc acgtccacca
     2161 gcttcgccag gttcgccgcc agccggcggg cgccagccgg atcggtggcg acaaacagcg
     2221 tcacgcggga gacgccggga gcctccgttc ggccgatcgt cagcgactcg acggcgaacg
     2281 cgcggcgcct gcagagcgac gcgatccgca tgagaacgtc cggcccgtcg cgtgcgagga
     2341 cgaccaggat gcgcgtcacc ggttacgccc ctttcccggc aggtgacggg accgcgcgcg
     2401 ggcggggttg cggccggcgc agcatctcgt gaaggctcgc acccgacggc accatcgggt
     2461 acaccaggtc gtcggcctcg accttgaact ccacgagaac gggccccgcc tgccggcgcg
     2521 cggctgcgat cgcggggctg acttccgccg gtgtctcgac gaggtgggcg ggaatgccgt
     2581 gggcctcggc cagcttgcag aagtcggggc ttgccatcgg cgtcgcggtg tagcgcttgc
     2641 cgtagaacag ctcctgccac tggcgcacca tcccgaggta cgcgttgttg atgaccgcga
     2701 tccggagcgg cagccgctcc tgcaccaccg tggacagctc gcaggccgtc atctggaacc
     2761 cgccgtctcc ggcgatcacc cagacctcgt cgccggggcg cgcgacctgc gccccgatgg
     2821 cggccggcag gccgaagccc atcgtgccca gcccgcccga ggtgatgaac gagcaggacc
     2881 gctcgggccg gtagtactga gcggcccaca tctggtgctg gcccacgtcg gtcaccacca
     2941 aagccttccc ccgcgtgtgc ttccacagtg cggagacgat gtgcgccccg tgcagccgcc
     3001 cgtcgccagg caggttgagg atgtcgcgcg cgccggccgc ccgacgcgcg gcgcgaatct
     3061 cgccgagcca ctcgtcgcgg ctgccgggtt cgacgagcgt gagcagggcg ggcagcacgc
     3121 gggcgaggtc gccctcgatg ccgacgtcca cctccaccac cttgccgatc tcggaggcgt
     3181 cgatgtccac gtggacgcgc cgcgccttcg gcgcgaatcc gcgcgcgttc ccgacggccc
     3241 gatcgtcgaa gcgggcgccg agcgacacca ccaggtcggc ttcctggacc gcctggttga
     3301 cccaggcgtc gccgtgcatc ccggccagcc cgaggttcag cgggtgcgag gcgggcagcg
     3361 cgccgaggcc gagcagcgtg gaggcgaccg ggatcccggc ccgctcggcg agcgcgcgca
     3421 gcgggcgcga cgagcccggc gcgatcgcgc catgcccggc caggatcacc gggcgccggg
     3481 ccgacgcgat gagcgccgcc gcggcgcggc acgccgccat gtcgagcgga ggggctgccg
     3541 ggcggcgagg ccgccgcgcg accggcgcgt actcctcgga cgcgcagcgc gactgctggg
     3601 catccttggt gatgtccacc agcaccgggc cgggccgccc ggacgaggcg acgtggaagg
     3661 cctcggcgat cgccggcgcg atggccgagg cgtcggtcac caggtagttg tgcttggtga
     3721 tgggcatcgt gatgccggtg atgtccacct cctggaacgc gtcgagcccg agctgcgacg
     3781 agcccacctg gccggtgatg cagacgatcg gcgagccgtc catcatcgcg gtggcgatgc
     3841 cggtcaccag gttggtggcc cccggccccg acgtggccat ggccacgccc acgcggccgg
     3901 tggcccgcgc gaagccgtcg gccatgtgcg cggccgcctg ctcgtggcgc accaggacgt
     3961 ggctgatgcc cgcgtgcggg agcgcgtcgt agatggggag gatggccccg cccgggtacc
     4021 cgaagacgtg cgtggtgccc agccgggcca gcgtctccca caccgcctgg gcgccggtga
     4081 gcgacggttc gggccggcgg gccgcctgcc ccgggaacga gatcttcggc ataggcctcc
     4141 tactcgagca cggcgccgag cgccgcggac gagaccgcgc gcgcgtagcg gttcagcaca
     4201 ccggcccggt agcggggagg gggctggcgc caggcggcga ggcgctcggc cacctgttcc
     4261 ggcgtcagct cgacttccag gaggcgttgc ccgatgtcga accggacgac gtcgccgtcg
     4321 cgcaccgcgg cgatgggtcc gaggcaggcc gcctcgggcg acacgtggcc ggccatcagc
     4381 ccccgcgtcg cccccgagaa gcgcccgtcg gtcacgaggg cgaccgagtc gccgaggccc
     4441 gccccgttga cggccgcggt cacggcgagc atctcgcgca tccccgggcc gccgcgcggc
     4501 ccctcgttgc ggatcacgat gacggtgccc ggcgcgatcc cgccgccttg caccgcctcg
     4561 aacgccgcct cttccgactc gaagacgcgc gcgggtccct cgtgctgccg gcgtgtgtac
     4621 cccgccacct tgacgacgca gccgtcgggc gccaggttgc ccttcaggat cacgagcccg
     4681 ccgctcggcg agatcggccg gtcgagcggg cgcacgacct gctgcccgtc cgcctcgcgg
     4741 gccccggccg ccgcctcgcc gatcgtcagg cccgagaccg tcgcgcaccc ggcgtgcagc
     4801 cgtcccgcct cgagcagccg cgccgcgacc agggcgctgc cgccggcgcg gtacaggtcg
     4861 gagaccgtga accggccgcc gggcttcagg tcggcgagca agggagtggc gccgctgatg
     4921 cggtcgaagt cgtcgatgcc gagcgcgacg cccgcctcgc gggcgatggc cagcaggtgc
     4981 agcacggcgt tggtggagcc gcccgtggcg gccacgcccg cgatggcgtt ctcgatcgag
     5041 gcgcgggtga tgacgtcttt cgggcgcagg ccgcgttcga gcagctgcat gacgagcgcc
     5101 ccggcccgcg cggccacgtc ggcccggccc gcgtcggccg ccgggacgct ggcgcttccc
     5161 atcggcgaga tcccgaggaa ctcgcaggcg agggccatcg tgttcgcggt gaactggccg
     5221 ccgcaggcgc ccggcccggg gcaggccgcg tcctcgatct cgcgaaggcc ctcgtcgtcg
     5281 aggcgtccgg ccgcgtgcgc gcccaccgcc tcgaagacgt cctggatcgt caggtcgcag
     5341 ccgccggcgc ggcccggggc gatcgcaccg ccgtagagca ccagcccggg gaggtcgagc
     5401 cgcgcgaggg ccaggacggc gccggggatg gtcttgtcgc agcccaccag cgccaccacc
     5461 gcgtcgaggc cgtgggcgcg cgcgaccagc tcgatcgagt cggcaatcac ctcccggctc
     5521 acgagcgacg cgcgcatccc ctcggtcccc atcgagatgc cgtcggaaac cgcgatggtg
     5581 ccgaactcga gcggcgtgcc gcccgcgcgg cggatgccct ccttgacgtg cccggccagc
     5641 tcgcgcaggt ggtagttgca ggggccgatc tcggtccagg tgttggcgat gccgacgagc
     5701 gggcgcgcca ggtcggcgtc ggtcaggcca atcgccttga acatcgcccg cgccggcgcg
     5761 cggctcgggc cgtcgagcgt cggcgcgctc tgcgcgcgca tcgggccatc gggtggcatg
     5821 gtgcctcgtc tcgggggacg gtgaaggccg gattgtgcgg ccgaggcggg gctacgtcaa
     5881 attaatagta cgcgcctgta cgataaatac cgcttatgga ataaggctgg gcatgggctg
     5941 gggggtgata gggcggtggt agggcggagg caaacggcgg cctcgcggga aggcgccggc
     6001 gacgcccgaa cacgtagggg gcgacccttg cggtcgcccg cgctgaatcc ggcgcggcaa
     6061 ggccgccgcc actgctacga cgggcgcttc ggctgcttct cctccttcgc ccgctcctgc
     6121 tcccggttct tgatcacgtg ctgcttctgg agcttcgact tgtccttctt gcccttgtct
     6181 cccatgaagc ctctcctcga atggggccgc cgcgcgcggc ccggaacgtc gaaacgtctt
     6241 gtgaaacgaa gcaagcgggg tgccagggca cgcggaccgg ttgccgccag ccccggctgc
     6301 cctgagcgaa ggcccgtgaa acaagtagcc cgggctagaa tggctccgca gtatcggcgt
     6361 cctctgtctc cggtgaggag gttgccctga cggagcctgt cccggtcgag cgcatcgctg
     6421 gtgatcgcga ggacctccgc cgcgccgagc aggaaggcat cggcctctgc ctctctggcg
     6481 gcgggtatcg cgcgatgctg ttccacctcg gggccctctg gcgcctcaac gaggcggcgc
     6541 tgctccccgg cctcgaccgg atctcgagcg tatcggccgg ctcgatcgcc gcgggagtgc
     6601 tcggccttcg ctggctcgag ctggagttcc gccacggggt ggccagccgc ttcgacgaac
     6661 tcgtcgcggc gccgctccgc gcgctcgccg gctggacgat cgacgagggg gcgatccttt
     6721 cgggcgcgct gctcccgggc gcgatcgccg agcgtctcga ggcggcgctc cgcgatcacc
     6781 tgttcgggga cgcgacactg caggaccttc cgcgcgactc ggagggcccg cgcttcgtca
     6841 tcaacgccac cagcgtccag acgggcagcc tctggcggtt ctcgaggccg ttcatggccg
     6901 actggcgcgt gggacggttc ctcgagcccc gcgtgccgct cgccttcgcg gtcgcagcgt
     6961 cgtccgcgtt cccgcccgtc ctctcaccgg cccgcctgcg gctcgttccc ggcatctaca
     7021 cgggcgtcga acccgccgag ctgttcggcc ctcccttcga ctcggaggcc gtcctgaccg
     7081 atggcggcgt gtacgacaac ctcgggctcg agaccgtctg gaagcggtac tcgaccgtgc
     7141 tggtgagcga cggcggcggc cgcctggagc ccgagggcga cccgaagcgc gactgggccc
     7201 ggcacgcgct cagggtgcac gagatcgtcg acaaccaggt tcgcagcctg aggaagcggc
     7261 agctgatcgg ctccttcagg gcgggatatc gaaggggcac ctactggagc attcgctgcg
     7321 acccggccga ctacggcgtt ccctcgctcg ccagcggcga ggtcacggca ggcctggccg
     7381 gcgtgaagac gcggctcgag gcgatggacg agaggctcca ggagcggttg gtcaactggg
     7441 ggtacgcagc ctgcgacgtg gcgctgcggg cgcacgtccg gccgggcctg ccgctgccgg
     7501 ccagctttcc gtacccgggc agcccgctgg acggccgccc gtgaggccgc aaccccggcc
     7561 gccgtttctc cgtccaattg ctcgacgcgg cacacgtgtt tggcccaggg ctcaaggctc
     7621 acggctcatg gtcggctcac ggctcgaggc tcccgtggag ttcacccgtt aacgagggaa
     7681 gcaagccaat gacgagcgca agaagggttg gcggtgccgt tctcggccgg ctcctcgccg
     7741 ccgcgatgat ggtgatcctg ccggcgctgc cggctcccgg ccaggaggcc gccgtcccga
     7801 agccctccgc cccgccggtc gaactgcccg ggaccgagcg gctttccctt cgctcccggg
     7861 tcaccggcca ggagtacgcc cttcacgtga gcctgccgcg ggggtacgac aagaccggcg
     7921 agcgcttccc ggtcatctac gcggtggacg gccagtggga cttcaccctg gtccacgcca
     7981 tctacgggca gcagtactac gacggctttc ttcccggtgc gattatcgtc ggcatcacct
     8041 ggggcggcga gaagccggac tacgatcggc gccgcgcgtt cgacctgacg ccgacctcgg
     8101 gcggccagcc cgatcgttac ggcaacgccc cgaggttcct cgagttcatc gagaaggagg
     8161 ccattccctt cgtcgaggcg agataccgtg cggccaggga cgggcgcacg ctcaccggca
     8221 gctcgttcgg cggcctcttc accctgtacg cgctcttcac ccggccggcc ctcttcgacc
     8281 gctacgtcct caccagcccc gcgtggtcat gggacaacca ggtcctcctg cgccacggcg
     8341 agcagttcgc caggtcgccg ctgccgaagc cggtgaaggt gttcatgggg gtgggggagt
     8401 acgaggacgt cgccggcttc gagaagctgg cggcgacgat ccggggctac ggccttgccg
     8461 ggctcgacct cgagaccagg gtgatcgcgg gggccggcca ctcgggcgcg aagaccgagg
     8521 ggttcacccg cggcctccag ttcgtgttcg cgcggccgtg cgcgcgcgtg cctcccgaag
     8581 cgttgtcgcg gctggccggc gagtacgagc tcgggcccgc tcccgccgcc gtcgtgagga
     8641 ttgccgtgga ggacggcggc ctggtcgcct acccgccctc gggcccgaag gtccgcctct
     8701 gcgccgattc cgacctcgac ttccgcctgg tcggccagta cctgaaggtg cacttcatcc
     8761 aggcggcgga caagacggtc ggcagtttcc ggctcgagca gtacgacggc gaggcggtgg
     8821 cgaagcgacg gaagtgatcg ggcgccgctg cgcgttccgc tacactctca gcctatgccg
     8881 acctcgatgg acgtactgcc tcgcgcgcag cgaccggcct ggacggttcg ctggtggctc
     8941 gcgatcgccg gcctgctgct cgcgcaggcc gcgatcctgc tggcgatggg gcgccttccg
     9001 gtctgcgagt gcggctacgt gaaggtgtgg cacggcgtgg tccacagctc cgggaactcg
     9061 cagcacgtct ccgactggta cacgttctcg cacctgatcc acggcttcct gttctactgg
     9121 gtgctgtcgc tggcgttgcc gcgctggaag gcgggctggc gggcggtgct ggcgctcggc
     9181 atcgaggtgg cctgggaagt gatcgagaac acccccttcg tcatcgaccg ctaccgggcg
     9241 gccacgattt cactcgacta ctacggcgac accgtcctca actcggtgag cgactcgatc
     9301 gcctgccttg cgggcttcgc ggccgcccgg aaactgccgg tcgcggccac catcctgctc
     9361 gcgctggcct tcgaggcctt cacgctctac tggatccgcg acaacctcac gctcaacgtg
     9421 atcatgctgc tctggccgct ggacgccgtc cgggcctggc aggcgggagg gtgagcgagg
     9481 ccctgcggtt ctgctagagt gtgctgccat gaggcacgcc ctggctccag caactctcgc
     9541 gctcgccgcg gttctcctgc tcggtgcggc ggcgcggcaa cagccggcgg ccccggaagc
     9601 cgatcgcggc gcccgccccg tcaagatcta cgtcaacacc gacctcgagg ggatcagcgg
     9661 cgtcttcaag ttcgcccaga cgcgcgagaa ggactcgccc gccaacatcc aggcctgcga
     9721 gtacttcatg ggcgacgtcg cggccgtcgt ccgcggcctg cgcgacggcg gcgcgaccga
     9781 ggtggtggtg ctcgacggcc acgggtcgca ggcggtgatc ccgcacctga tggcgcctgg
     9841 cgccgtctac atcaccggca ccccgcggcc cggcgcgggc aacctttccg agctcgactc
     9901 gtcgttcgcc ggcatggtga tgctcggctt ccacgcgatg atgggaacgc ccgacggcgt
     9961 cctgaaccac acccagtcgt cccgcaccga gaaccgctac tggtacaacg gcgtcgagtc
    10021 gggcgagctg gtgcagaacg cggcgatcgc gggccactac ggcgtcccgc cgatcctggt
    10081 gacgggcgac gaggcgacct gccgggaggc gaagcggttc ctcggcgacg acatcgtcac
    10141 ggtggcggtg aagaagggcc tggcgcggga gtcggcggtg ctgtacccgt tcgaggagac
    10201 gcggaaggcg ctctacgaag gggcgcgccg ggcgatgacg acgatcggct cgcgccggcc
    10261 gtacgttttg aagacgccga ttgccgtgaa ggagcagttc ctcgacctcg agccgtcgct
    10321 cccgaaaccc cggctcgtga cgcgcgaggg ggtcgcccgc gacgcgctgc acctgctctc
    10381 gtgggcgaag gagcctcgct gaaacaatcc tcccaccctg gcgcgggtcc aaccggggtc
    10441 ccacgcgccg gccggcctgt ccgtcgtgca cgaaaaatgc cgggcggcct tgcgccgacc
    10501 cgccggctga cgataaggaa aaggaagatg acgcccctac ggctcgaacc cgccgtgtcc
    10561 ctcctcaggg ggacgccggc agtcaccccg gcacagggtt ggaccgagga cgcgatgacg
    10621 gaatggggag ttcaactggc gatggttcgg ctcgccgacg ccatccgcca gctgggggat
    10681 ggcgatcggg agcggcaggt tcgggcggcg tttcttcacg tcgtcgagcg tctccaggaa
    10741 ggcgccgccc tggtcacccg cgactgggac gtgggcgcgc ggcaccagga cgcgccgctg
    10801 tggacgctca ccgacggcgc gagccgcacg cgggcggtgt tgcggttcgt cgaggactac
    10861 ggcttcgaag tgcgggtctt cgtggacgac gagctgcgcc aggcaaggat gttccggcac
    10921 gaccgcgagg ccgccctgca gttcgccagg acgcggcgcg cgggcctgga gacgcgcggg
    10981 tggagggaat ggacggtcgt gcccggttcc ccggaggccg gcggggatcg ggcggtttcc
    11041 ctcc
//
LOCUS       DAOPQX010000091        10739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7006_length_10739_cov_3.997566, whole genome shotgun sequence.
ACCESSION   DAOPQX010000091 DAOPQX010000000
VERSION     DAOPQX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7006_length_10739_cov_3.997566"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1254
                     /locus_tag="PKK95_06860"
     CDS             94..1254
                     /locus_tag="PKK95_06860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC17968.1"
                     /translation="MRESAAAEPLRVALVAPTLGILGGQAVQARRLLTGWAGDPEVRA
                     WLVPVNPTPPGPLAHALRVKYARTVVTQVAYWPSLVRQLARADVVHVFSASYLSFVLA
                     PLPATLVARALGKPVVLNYRSGEAPDHLARSALARRVIRSCDRNVVPSSFLAEVFAGH
                     GIPSVIIPNTIDLDRFAFRARQPLRPRLLSTRNLEPMYNVACTLKAFAIVQARHPDAT
                     LTLAGAGSQEGRLRALASSLGLRGVRFAGRIAQEEIWKLYADADIYVQSPDIDNMPSS
                     VLEAFASGNPVVSTDAGGVPAVVHHETNGLLTPRDDHHRLAAAVLRLLDEPSLASRLA
                     GAARNECERYSWPAVRQQWLDLYREVARARRGAAITRAARSSGYGATPAGQA"
     gene            1251..3728
                     /locus_tag="PKK95_06865"
     CDS             1251..3728
                     /locus_tag="PKK95_06865"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOC17969.1"
                     /translation="MNAMRLAARLKRMSGAELAFRLRVAARSEAGRVAAACRPPRWRV
                     AALAGLLDSTSPLVREAAARLEAGNLPAAHACLARHFRTRENRFPVAATVLPVVRERI
                     LSEFPDAAAEAAADIGRLQRGRFNLLGYRDLDFSDCVSRRAEGTGVSRRPGGVDWQID
                     PVHGRRPPLTWWSRVPFLDPAVGDHKIIWELNRHQHFLRLGRAAWLTGDPACRRTFAG
                     HLRGWLEQNPPGMGINWSSMLELGLRSLSWLWALHLFSSQPAAEKAAHGDGADPWIPG
                     LLAGLHAQLQQVLRNLSRYFSPNTHLTGEALALYVCGRALPELRDAAGWATAGRQILL
                     EQIGVQVTPDGGHCERATHYLRYTLDFYLMALAFARATADGAATGAFEEACERLAWAT
                     RLLSDEGGRLPNIGDDDGGRLWPLREREPGDVRDSLATAAILLDRPGLLVGRLPEEPL
                     WLLGPSLAWPGAQPSHGDGDRRAAVCGAEGVAMGYHVARPRPGDHLVIDAGPHGFLNG
                     GHAHADSLSIVYTVGNRPLAIDPGTFTYTMSREWRDRLRSSAWHNTLTVDGRSQSVPD
                     GPFHWRNAAAAHAQASERGEGYWFFEGVHDGYLPLVHRRRVAALDSGCLVVVDTVVGT
                     GDHLAEVHWHLDGAWKARREAEGVIVLDHELGGSCQIATPGNTPAVLVADEATGLGWR
                     SPAYGRLVPCTTARLAAAGRLPLVLVSVFADWDGVPLRVERLQPASASAGGSGDGLAV
                     RLTAGRHSDVLLFGTYPPLLPGEDAEAPRDAAAAVHRFGLGRFEVTTSARFAWLAGEA
                     EASPRVLALVDGNAVVEASAPGVPGSA"
     gene            3738..5627
                     /gene="asnB"
                     /locus_tag="PKK95_06870"
     CDS             3738..5627
                     /gene="asnB"
                     /locus_tag="PKK95_06870"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOC17970.1"
                     /translation="MCGIAGFVEVDGSPAEPREALVRRMCAAIRHRGPDDEGVHVDGH
                     AALGMRRLAIIDVNTGQQPMSGEDGRIVVVFNGEIYNFQELAEELRGAGHRFRTRSDT
                     EVIVHAWEQWGEDALARLRGMFGIAVWDIASRTLWLARDRAGIKPLHYAQSGGRLVFG
                     SEIKSLLADPRVPRDIDPASLDHYCSFLYTPPDRSIFAAIRKLPPGHVLRWNDGQVST
                     RQYWDLPDESFRGSEQEAAARLRELLGDAVRSHMISDVPLGAFLSGGIDSSAVVALMA
                     RASGQRVKTFSIGFEEATHDELPYAREVARLFDTDHHEFVVRPDALSILDRLIAHFDE
                     PFGDSSAIPTWYVSEIAGRHVTVVLSGDGGDELFGGYRRYRPHERVAAFDRLHLPGRI
                     SAARLAWRHWPRGLRGRNFLRHVAQDAEGRYLEDIGFFRPDEKNVLYTPAFAAAVAGS
                     DPFASMRRLLDAERNVSWASRMMRFDFRTYLPNDGLTKVDRMSMAHSIESRVPLLDTA
                     VVEFAATLPASFKITADGRQKHVLKQAVADLLPRSILDRKKQGFGVPIGRWFGRDLRE
                     LFSDTLLSPRAAGRGYFEPREVRRLVGEHVGGRRDHTLRLWLLMVLELWQRQYVDAQA
                     GAAAA"
     gene            5792..6937
                     /locus_tag="PKK95_06875"
     CDS             5792..6937
                     /locus_tag="PKK95_06875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17971.1"
                     /translation="MPPARLPVAVFLTSFDPGGTERQMIELVRRLDRARFEVHVACFH
                     RRGEWRARAEELAASVVEFPIGGFARAATARQAARFAAWCRRLRLAAVQACDLYTNIF
                     ALPAAAVAGVPVRIGSRRGLNPDRTRWHRLAQQASYRFADRIVANSEAVARGMRESKV
                     AARRVVVIPNGLDVARFRPVGRAGGIRRIGTLARLHPVKGLDTFLEAVALLHRDHFEV
                     EAQIVGDGPQRAGLERQAAALGLSGRAHFAGHREDVETVLAGFDLFVLPSRSEAFPNA
                     VVEAMASALPVVATEVGGVPEIVSHGLTGSLVPPGRPDLMAAAIRELVLQPNRARAMG
                     RRARAAVVQRHSFERTIASFEALYLEVAAGKRVRVAAGAAPEAAAAR"
     gene            6962..8911
                     /gene="asnB"
                     /locus_tag="PKK95_06880"
     CDS             6962..8911
                     /gene="asnB"
                     /locus_tag="PKK95_06880"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOC17972.1"
                     /translation="MCGIAGIVGARRLAPGDADRARRMTGVLEHRGPDEEGFHLDDQA
                     ALGHRRLSIIDLATGQQPLCNEARSIWIVYNGEVYNHADLRSELESRHRYRTRSDTET
                     IVHAYEEWGDRAVDRLRGMFAFAIWDSRRCRLLLARDRLGIKPLYWALQGDRLVFASE
                     IKAILESGLIPAEPNEERISEILATRYLSDSETLFRGIHRLPPGHQLFFENGRVRVVQ
                     YWDLPLDPPANAGEETSGRGAARTVARFRDLLEESVRLRMMSEVPLGVFLSGGIDSSV
                     IAALTARQLSRPLETFSVAFADRAFNELQYSRQVADAIGANRHEVVIDEHDFFGALPR
                     LVWHEDEPIAHPSSVPLYFVSRLAREHVTVVLTGEGSDELLAGYGKYPRALWNWRLGG
                     AYTRVIPPAIRAVVAGRIVPGLPASAARYAKRSFLAVPHTPEAAFFDNFAALPLAAQR
                     RLLSPRMSALATPEAAYGPSLRVFEAARRLPLLQRVLYTDIKTYLVELLMKQDQMSMA
                     TSIESRVPFLDHVLVEFAASLPARWKLSGLTTKRILREAARGLLPREILERPKMGFPV
                     PLGRWLRGGWGEAVREVLLDRRSRERGLTAPAEIESLLAAHRAGRTDAADAVWSLLNL
                     ELWYRTFVDGDGVQVLPAPVRAVAA"
     gene            8950..10221
                     /locus_tag="PKK95_06885"
     CDS             8950..10221
                     /locus_tag="PKK95_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC17973.1"
                     /translation="MRVLWLNAGLLLPLDKGGKLRTWHLMRHLAARHEVTYLSFAEPG
                     ASSADLAGMREVCSRLETVPRREVSKGSAAFYLRASRFLLDPAPYAVAAYRSAQYRAR
                     AAALLADGGYDAVVCDFLVPAVNLPRRLPCPAVLFTHNVEAEIWRRHAETAATAWRRA
                     LMRSQWRRMLRFEGDALARFEMVLAVSDADRDTFARLYGGRLRAPVRVIPTGVDARYF
                     AEAADLPYDSHRLAFTGSMDWLPNEDAVLYFGREILPEIRKVEPRVTLSIIGRNPTPA
                     VHRLAAEVPGIEITGRVEDVRPHVARASLSIVPLRIGGGTRLKIYEAMAMGRPVVSTS
                     VGAEGLPLVAGRDLEIADGPRAFAARVLSLLSDPAARQRMADAARRLVVERYDWSAVA
                     GTLEEAIHAAATGGLAKAPVPQLLGEVRSRS"
     gene            10218..>10739
                     /locus_tag="PKK95_06890"
     CDS             10218..>10739
                     /locus_tag="PKK95_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOC17974.1"
                     /translation="MKVSVFGLGYVGCVSAAALAGDGHEVVGIDVNPDKVDAVNRGHS
                     PIVEPGLHEAVGAAARAGRLRATTSTAEAVRESEVSLLCVGTPSRRNGSLDLQYLSRV
                     SEQIGDALRDKAGYHVVVVRSTVLPGTTHDVVIPALERHSGKRYGEDFGVAVNPEFLR
                     EGSALNDFREPPIT"
BASE COUNT         1409 a         3844 c         3874 g         1612 t
ORIGIN      
        1 gttcctggtc gagtgctggc agcggggctc gcggcgcagc cgcggcactg gcgcgccgag
       61 agcggccgaa ccacacaaga cggcggtggc gggatgagag agtcggcagc agcagaaccg
      121 ttgcgggtgg cgctggtcgc cccgacactc ggaatcctcg gcgggcaggc cgtacaggcg
      181 aggcgcctgc taacgggctg ggctggcgac cctgaagtgc gcgcctggct ggtgccggtc
      241 aacccgacac ccccgggtcc gctggcgcac gcgctccgcg tgaagtacgc ccgaaccgtt
      301 gtcacgcagg tcgcgtactg gccgtcgctc gtgcggcagc tcgcgcgcgc cgatgtcgtg
      361 cacgtcttct cggcgtcgta cctctcgttc gtcctcgcgc cgctgccggc caccctggtc
      421 gcccgcgcac tgggtaagcc cgtggtgctg aactaccgga gcggcgaggc acccgatcac
      481 ctcgcccgct cggcgctggc ccgccgcgta atccgctcct gcgatcgcaa cgtggtgccg
      541 tcgtcgttcc tggcagaggt gttcgccggg cacggcattc cctcggtgat catccccaac
      601 acgatcgacc tcgatcggtt cgctttccgg gcccggcagc ccctgcgccc gcggctgctg
      661 tccacgcgga acctcgagcc catgtacaac gtggcgtgca ctctgaaggc gtttgcgatc
      721 gtgcaggcgc ggcacccgga tgccaccctg acgctggcag gggcgggctc gcaagagggc
      781 cgcttgcgcg cactggcctc gtcgctgggg ctgcgcggcg tgcggttcgc cggacgcatc
      841 gcgcaggagg agatctggaa gctctacgcc gacgccgaca tctacgtgca gtcgcccgac
      901 atcgacaaca tgccctcgtc ggtgctcgag gcgttcgcca gcggtaaccc ggtcgtctcg
      961 acggacgccg gcggcgtgcc cgccgtcgtg caccacgaaa ccaacggact gctcacgccg
     1021 cgcgacgacc atcaccggct cgcggcggcg gtgctgcggc tgctcgacga accctcgctc
     1081 gcctcgcgcc tggccggggc ggcgcggaac gagtgcgaac gctacagctg gcccgcggtg
     1141 cggcagcagt ggctcgactt gtatcgcgag gtggcgcggg cgcgacgcgg cgccgcgatc
     1201 acacgggcgg cgcgcagcag cggctacggc gcgacgccgg cggggcaggc atgaacgcga
     1261 tgcggctcgc cgcgaggctg aagcgcatga gcggcgccga gctggcgttt cgcctgcgcg
     1321 tggcggcaag gtcggaagcc ggccgcgtgg cggccgcctg ccgcccgccc cgctggcgcg
     1381 ttgccgccct ggccggcctg ctcgattcaa cctcgccgct cgtacgcgaa gccgccgccc
     1441 gcctcgaggc cggcaacctg ccggcggctc atgcctgcct ggcccgccac ttccgtacgc
     1501 gcgagaaccg cttccccgtc gcggccaccg ttcttcccgt cgtgcgcgag cggatcctct
     1561 cggaatttcc ggacgccgcc gccgaggccg ctgccgacat cgggcgtctc cagcgcggcc
     1621 gattcaatct gctcggctac cgcgacctcg acttctcgga ctgcgtgtcg aggcgggcgg
     1681 aaggaaccgg cgtgtcgcgg cggccgggag gggtcgattg gcagatcgat cccgtgcatg
     1741 gacgccggcc gccgttgacc tggtggtcgc gggttccctt tctcgacccg gccgtcggcg
     1801 atcacaagat catctgggag ctgaaccgcc accagcactt cctcaggttg gggcgcgcgg
     1861 cgtggctgac gggagacccg gcgtgccgcc gcaccttcgc cggccacctt cgggggtggc
     1921 tcgagcagaa cccgccggga atgggcatca actggtcgag catgctcgag ctcggcctgc
     1981 ggtcgctctc gtggctctgg gccctgcacc tgttctcgag ccaaccggcc gcggagaaag
     2041 ccgcgcacgg cgatggcgcg gatccctgga ttcccggcct gctggccggg ctgcacgccc
     2101 aactgcagca ggtgctccgg aacctctctc gctacttcag cccgaacaca cacctcaccg
     2161 gggaggcgct cgccctttac gtgtgcggcc gggcgctgcc cgagcttcgg gacgcggccg
     2221 gctgggccac cgcgggccgg caaatcctgc tcgagcagat cggcgtgcag gtgacgcccg
     2281 acggcggcca ctgcgagcgc gcgacccact accttcgcta cacgctcgac ttctacctca
     2341 tggcgctggc cttcgcgcgg gccaccgcag atggcgccgc caccggcgcc ttcgaggagg
     2401 cgtgcgagcg gctcgcctgg gccactcgcc tgctgagcga cgaagggggc cggctgccga
     2461 acatcggcga cgacgacggg ggacggttgt ggccactgcg cgagcgcgag cccggcgacg
     2521 tgcgagactc actggccacc gccgccatcc tgctcgatcg cccgggcctg ctggtcggcc
     2581 ggcttcccga ggagccgctc tggctgctgg ggccaagtct tgcatggccg ggtgcgcagc
     2641 cctcgcacgg cgacggcgac agacgggctg cggtgtgcgg ggccgaggga gtggccatgg
     2701 ggtatcacgt ggcacgcccg aggcccggcg atcacctcgt catcgacgcg ggtcctcacg
     2761 gcttcttgaa cggcgggcac gcccacgccg actcgttgtc gatcgtctac acggtaggca
     2821 acaggccgct cgcgatcgac cctgggacct tcacctacac gatgtcgcgg gagtggcgcg
     2881 accggctgcg atcgagcgcc tggcacaaca cgctcaccgt cgacggacgc tcccagtcgg
     2941 tgcccgacgg cccgttccac tggcgtaacg cggcggcggc ccacgcgcag gcatcggagc
     3001 gaggcgaggg gtactggttc ttcgaaggcg tgcacgacgg ctaccttcca ctcgtccaca
     3061 ggcggcgggt cgccgcgctc gattccggct gcctcgtggt cgtggacacg gtggtcggca
     3121 ccggggatca cctcgccgag gtccactggc atctcgatgg ggcctggaaa gccaggcggg
     3181 aggccgaggg ggtgatcgtg ctcgatcacg agctgggggg cagctgtcag atcgccacgc
     3241 cggggaatac cccggcggtc ctggtggcgg acgaggcaac cggtctcggc tggcggtcgc
     3301 cggcctacgg ccgcctcgtg ccgtgcacga cggcccggct cgcggctgcc ggccggcttc
     3361 cgctcgtgct ggtgtcggtg ttcgcggatt gggatggcgt tcccctgcgg gtcgagcgcc
     3421 tgcagccagc gagcgcgtca gccgggggat cgggcgacgg cctcgcggtg cggctcacgg
     3481 caggacgcca cagtgacgtg ctgctcttcg gcacctaccc tccgcttctc cccggcgaag
     3541 acgcggaggc cccgcgcgac gcggcggcgg ccgtgcatcg cttcggcctg ggacggttcg
     3601 aggtgacgac ctcggcgcga ttcgcctggc tggccggcga ggccgaggcg tcgccgcggg
     3661 tgctggctct ggtggatgga aacgcggtgg tggaggcgtc cgcgcccggc gtgccgggtt
     3721 cggcgtgagg gacgatcatg tgcgggatag caggcttcgt cgaggttgac gggtcaccgg
     3781 ccgagccgcg cgaggcgctg gtccggagga tgtgcgcggc cattcgtcac cgcggcccgg
     3841 acgacgaggg ggtgcacgtc gacggccatg ccgccctcgg gatgcggcgg ctggcgatca
     3901 tcgacgtcaa cacgggccaa cagccgatga gcggcgagga cggccgcatc gtggtcgtct
     3961 tcaacggcga gatctacaac ttccaggaac tggcggagga gctgcggggc gcgggccacc
     4021 gcttccgcac ccggagcgac accgaagtca tcgtgcacgc ctgggagcag tggggcgagg
     4081 acgccctcgc ccgcctgcgc gggatgttcg ggatcgcggt ctgggacatc gcgtcgagaa
     4141 cgctgtggct cgcacgtgac cgcgccggga tcaagccgct gcactacgcg caatcgggcg
     4201 ggcggctcgt cttcggctcc gagatcaagt cgctcctggc cgaccccagg gtgccccgcg
     4261 acatcgaccc ggcctcgctc gaccactact gctcgttcct ctacacgccc ccggaccggt
     4321 cgatcttcgc cgcgatccgc aagctcccgc ccggccacgt gctgcgctgg aacgatggcc
     4381 aggtctcgac ccggcagtac tgggatctcc cggacgagtc gttccggggg agcgagcagg
     4441 aggcggccgc acggctcaga gagctgcttg gcgacgcggt tcgctcgcac atgatcagcg
     4501 acgttccgct cggggccttc ctgtcgggcg gcatcgactc gagcgcggtg gtggcgctga
     4561 tggcccgggc ctcggggcag agggtgaaga ccttctcgat cggcttcgag gaggcgaccc
     4621 acgacgagct gccgtacgcc cgcgaggtgg cgcgcctgtt cgacaccgac catcacgagt
     4681 tcgtcgtgcg gccggacgcg ttgtcgatcc tcgaccggct gatcgcccac ttcgacgagc
     4741 cgttcggcga ctcgtcggcg atccccacgt ggtacgtctc ggagattgcg gggcggcacg
     4801 tcacggtggt gctgtcgggc gacgggggcg acgagctgtt cggagggtac cgccgatacc
     4861 ggccgcacga gcgggtggcc gccttcgacc gcctgcacct gcccgggcga atctcggcgg
     4921 cccgcctggc gtggcggcac tggccgcggg ggcttcgcgg ccggaacttc ctgcggcacg
     4981 tcgcccagga cgcggaaggc cgctacctgg aggacatcgg cttcttcagg cccgacgaga
     5041 agaatgtgct ctacacgccg gcgttcgcgg cagcggtggc cggctcggat cccttcgcgt
     5101 ccatgcgccg cctgctcgac gccgagcgga acgtgtcgtg ggcgagccgg atgatgcgct
     5161 tcgacttccg cacctacctg ccgaacgacg gcctgacgaa ggtggatcgg atgagcatgg
     5221 cgcactcgat cgagtcgcgc gtgccgctgc tggacaccgc cgtggtcgag ttcgcggcga
     5281 cgctgccggc gtcgttcaag atcacggcgg acggacgcca gaaacacgtg ctcaagcagg
     5341 cggtcgccga cctgctgccg cggtcgatcc tcgatcggaa gaagcaggga ttcggggtgc
     5401 cgattggccg ctggttcggc cgtgacctgc gcgagctgtt ctccgacacc ctgctgtcgc
     5461 cgcgcgcggc cgggcgcggc tacttcgagc cccgcgaggt gcggcgcctc gtgggcgagc
     5521 acgtcggggg ccggcgggat cacacgctcc ggctgtggct gctgatggtg ctcgagctgt
     5581 ggcagcgcca gtacgtggac gcacaggccg gcgccgcagc ggcgtgacgc gagcccggca
     5641 gaacgccagc ccgttccgcc tttcgccggt cccgagaggc gcagatgtgg aagcgccgtt
     5701 ccaccgcgag cgccacgcgc cgcccccgtg gttccggaag gcgggtttcc cattccgttg
     5761 ggctggcacc tcccttgctg acctcgtcgg catgccccca gcgcgcctgc ccgtcgccgt
     5821 cttcctgacg agcttcgatc ccgggggaac cgagcgccag atgatcgagc tggtccgccg
     5881 gctcgaccgc gcgcgcttcg aggtgcacgt cgcgtgcttc caccggcgcg gcgagtggcg
     5941 cgcccgtgcg gaggagctgg ccgcgtcggt ggtcgagttc ccgatcggcg gcttcgcccg
     6001 cgcggcgacc gccaggcagg ccgcgcggtt cgccgcctgg tgccggcgcc tgcgcctggc
     6061 cgcggtccag gcctgcgacc tctacacgaa catcttcgca ctgcccgccg cggccgtggc
     6121 cggcgtcccc gtgcgaatcg gcagccgccg cggcctcaac cccgatcgga cccgctggca
     6181 ccgcctcgcc cagcaggcct cctatcgctt cgcggatcgg attgtcgcga actccgaggc
     6241 tgtggcgcgt ggtatgcgtg agtcgaaagt cgccgcgagg cgcgtggtgg tcattccgaa
     6301 cggcctcgac gtcgcacggt tccggcccgt cggacgcgcg ggtggcatcc gccgaatcgg
     6361 gaccctggcg cgcctccacc ccgtcaaggg cctggacacg ttcctcgagg ccgtcgcgct
     6421 gttgcaccgg gatcacttcg aagtcgaggc gcagattgtc ggcgacgggc cccagagggc
     6481 cggcctcgaa cggcaggccg cggcgctcgg cctgtcgggc agggcgcact tcgcgggaca
     6541 ccgcgaggac gtcgagaccg tgctcgcggg tttcgacctg ttcgtcctgc cgtcgcgctc
     6601 cgaggccttt cccaacgccg tggtcgaggc gatggcctcg gccctgccgg tggtcgccac
     6661 cgaggtcggg ggggtgcccg aaatcgtctc gcacggtctg acggggtcgc tggttccccc
     6721 cggccggccc gacctgatgg ccgcggcgat ccgcgagctc gttctccaac cgaaccgggc
     6781 gcgcgcgatg ggacggcggg cgcgcgcggc ggtcgtgcag cgccactcgt tcgagcggac
     6841 gatcgccagc ttcgaggcgc tctacctcga ggtcgcggcc ggcaagcgcg tgagggtggc
     6901 ggcaggcgcg gcgcccgagg cggccgcggc acgctgaggc ggcgaaagga gttgaggcaa
     6961 cgtgtgcggg attgcgggaa tcgtgggggc gcgccggctc gcgccgggtg acgccgaccg
     7021 ggcgcggcgg atgacgggcg tgctggagca ccgcggcccg gacgaggaag gctttcacct
     7081 cgacgaccag gcggcgctcg ggcaccggcg cctcagcatc atcgacctgg caaccggcca
     7141 gcagcccctc tgcaacgagg cgcggtcgat ctggatcgtc tacaacggcg aggtctacaa
     7201 ccacgccgac ctgcggtccg agctcgagtc gcggcaccgc taccgaaccc ggtcggacac
     7261 cgagaccatc gtgcacgcct acgaggagtg gggcgaccgc gcggtcgacc ggctgcgggg
     7321 catgttcgcc ttcgcaatct gggacagccg ccgctgccgt ctcctgctcg ctcgcgaccg
     7381 cctcggcatc aagccgttgt actgggcgct gcagggcgac cgcctggtgt tcgcctccga
     7441 gatcaaggcc atcctcgagt cgggcttgat tccggcggag ccgaacgagg aacggatctc
     7501 cgagatcctc gccacgcgct acctctcgga tagcgagacg ctctttcgag gcatccaccg
     7561 ccttccgccg gggcaccagc tcttcttcga gaacggccgc gtgcgggtgg tgcagtattg
     7621 ggatctcccc ctcgatccgc cggcgaacgc cggggaggaa acctccggcc gcggcgctgc
     7681 ccggacggtt gcccggttcc gcgacctgct cgaagaatcg gtccggctgc ggatgatgag
     7741 cgaggttccc cttggggtct tcctctcggg cggcatcgac tcgagcgtga tcgccgcgct
     7801 gaccgcgcgc cagctgagcc ggcccctcga gaccttctcg gtggccttcg ccgaccgtgc
     7861 gttcaacgag ctgcagtact cgcggcaggt ggccgacgcg atcggcgcga accgccacga
     7921 ggtggtgatc gacgagcacg acttcttcgg cgccctcccg cgcctcgtgt ggcacgagga
     7981 cgaaccgatc gcgcacccct cgtcggtgcc gctgtacttc gtctcgcggc tcgcccgcga
     8041 gcacgtgacg gtggtgctga ccggcgaggg cagcgacgag ctgctggccg gctacggcaa
     8101 gtacccgcgg gcgctgtgga actggcgcct tggcggcgcg tacacccggg tcatcccccc
     8161 ggccatccgc gcggtggtcg cgggccggat cgtgccgggg ctgccggcgt ccgcggcgcg
     8221 gtacgcgaag cgatcgtttc tcgcggtgcc gcacaccccg gaggcggcgt tcttcgacaa
     8281 cttcgcggcg ctgccgctgg ccgcccagcg ccgcctgctg tcgccccgga tgtccgcgct
     8341 ggcaacgccc gaggccgcct acggcccctc gttgcgcgtg ttcgaggcgg cccggcgcct
     8401 gccgctcctc cagcgtgtgc tctacacgga catcaagacg tacttggtcg agctgctgat
     8461 gaagcaggac cagatgagca tggctacctc gatcgagagc cgggtgccct tcctcgatca
     8521 cgtgctggtc gagttcgccg cctctctgcc cgcccggtgg aagctttccg gcctgaccac
     8581 caagcgcatc ctccgcgagg cggcccgggg gctgctgccg cgcgagatcc tcgagcgccc
     8641 gaagatgggc ttccccgttc cgctcgggcg gtggctgcgc ggcggctggg gcgaggcggt
     8701 gcgggaggtg ttgctcgacc ggcggtcgcg cgagcgcggg ttgaccgcgc cggccgagat
     8761 cgagtcgctg ctggccgccc acagggccgg ccggaccgac gcggcggacg ccgtctggag
     8821 cctgctcaac ctcgaactgt ggtatcgcac cttcgtcgat ggtgacgggg tgcaggtgct
     8881 gcccgcgccg gtacgcgcgg tcgcggcata ggaagcgttc gggaccacgc ggggacacga
     8941 ggaaacacga tgcgcgtgct gtggctgaac gccgggttgc tgctgcccct cgacaagggg
     9001 ggcaagctcc gcacctggca cctgatgcgg cacctcgcgg cccgccacga ggtgacctac
     9061 ctctcgttcg ccgagccagg cgcctcgagc gccgacctgg ccggcatgcg cgaggtgtgc
     9121 agccggctcg agaccgtgcc gcgccgtgaa gtgtccaagg gatcggcggc cttctacctc
     9181 cgcgcgtcgc gctttctgct cgaccccgcg ccttacgccg tcgcagccta ccggtcggcc
     9241 cagtaccgcg cccgcgccgc cgcgctgctc gccgatggcg ggtacgacgc cgtcgtctgc
     9301 gacttcctcg tccccgccgt caacctgccg cggcgcctcc cctgccccgc cgtgctcttc
     9361 acgcacaacg tggaggccga gatctggagg cggcacgccg aaaccgccgc gaccgcgtgg
     9421 cgccgggccc tgatgcgctc ccagtggcgg cgcatgctgc ggttcgaggg ggacgccctc
     9481 gcacgcttcg agatggtcct ggcggtctcg gatgccgacc gggacacctt cgccaggctg
     9541 tacggagggc ggttgcgcgc gccggtccgc gtcatcccga ccggagtgga cgcgaggtac
     9601 ttcgccgagg ccgccgacct tccctacgac tcgcaccgcc tggccttcac gggctcgatg
     9661 gactggctgc ccaacgagga tgccgtcctg tacttcggcc gcgagatcct gccggagatt
     9721 cgcaaggtcg agccgcgcgt gacgctctcg atcatcggtc gcaaccccac gcccgccgtg
     9781 caccgcctgg cggccgaggt gcctggcatc gagatcaccg ggcgcgtcga agatgtccgg
     9841 ccccacgtgg cgcgcgcctc gctgtcgatc gtgcccctgc gcatcggcgg cggcacgcgc
     9901 ctgaagatct acgaggcgat ggccatggga cggcccgtcg tctccaccag cgtcggcgca
     9961 gaggggttgc cgctggtcgc cggccgcgac ctggaaattg ctgacggccc ccgtgcgttc
    10021 gccgcacgcg tgctgtcgct gttgtccgat cccgcggccc gtcagcgcat ggccgacgcg
    10081 gcgcgccgcc tcgtggtcga gcggtacgac tggtcggcgg ttgccggcac cctcgaggag
    10141 gccatccacg cggccgcgac cggcggcctc gccaaggcgc ccgtccccca actgctcggc
    10201 gaagtgaggt cccgatcatg aaagtctccg tcttcggtct cggctacgtg gggtgcgttt
    10261 cggctgccgc tctggccggc gacggccacg aggtggtggg catcgacgtc aaccccgaca
    10321 aggtggacgc cgtgaaccgg ggacacagcc cgatcgtcga acccggactc cacgaggcgg
    10381 tcggggctgc cgccagggcc ggccggctgc gggccacgac gagcacggcc gaggcggtgc
    10441 gggagagcga ggtctcgctg ctctgcgtcg ggacgccgag ccgccggaac ggcagcctcg
    10501 acctgcagta cctgtcgcgc gtctcggagc agatcggcga cgccctccgc gacaaggcgg
    10561 gctaccacgt ggtcgtcgtg cgcagcaccg tgctgccggg caccacccac gacgtggtca
    10621 tccccgccct cgaacggcac tcgggaaagc gctacggcga ggacttcggc gtcgcggtca
    10681 accccgagtt ccttcgcgag ggcagcgcgc tgaacgactt tcgcgagccg cccatcacg
//
LOCUS       DAOPQX010000092        10719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7023_length_10719_cov_4.197768, whole genome shotgun sequence.
ACCESSION   DAOPQX010000092 DAOPQX010000000
VERSION     DAOPQX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7023_length_10719_cov_4.197768"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..2135
                     /locus_tag="PKK95_06895"
     CDS             45..2135
                     /locus_tag="PKK95_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17975.1"
                     /translation="MGALFDPEDVAWTAPLLALWVSPLAILAAVPGVAQVFPAFAFVL
                     VDLRWWWTAAIAAATLVGAWRRVRGTPPGDTPPRGDTLPSGRLAVPRLPPALALAALC
                     GAWVVLGTPHLRFSPVVHGDEAKYLRYCENFYQGHGLEISSIRPIAQLPPGFAPRFGR
                     NLELAAQVLPGELRNLAADARVFLSDPSHKFARSVKARGFVRGKNGGVYQVHQPGLSV
                     LMLPAYSLDRWLSVPGRRSPRQWPPRLVAVNAFFLSLYLVWVVLVYRLAVRLTGDSRV
                     AATSALAVGATLPVAAFPFQFYPEAAAGIVVIAVAGHVFFPLRRTPAAALAAGFATGC
                     LPWLHVRFVPLAVLLAAFAMIAGRREGRRVAGFVSGAAIPTAFLLLYVYRVTGSILPT
                     SLWAGESGALPLSALTAVPGSVAYLLDRRWGLLAHAPIYLLALPGWWLLARQRRDLAW
                     FCALGFLAVLLPAAAHTLHAAATTPCRLIAAVVPLAALPLAVLLTRSSGRRWLKAAFA
                     VLLALSVQNALAYNWWHVKPVGWLVDQSFSGWKVNLLFPARGATPWVTASNGWLYVAW
                     VLVLVAAFAAPLWFERRGGETTARAGRAQLPAVAAALALVALLGTAVAAATGSWEEND
                     YRRPNAGAAGRAAALLLQPGERLRLSSERGRIGADAALAQLATVHPSLAGPPGGEAAE
                     PSPPDEEENSSAPR"
     gene            complement(2159..3547)
                     /locus_tag="PKK95_06900"
     CDS             complement(2159..3547)
                     /locus_tag="PKK95_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC17976.1"
                     /translation="MKKKPTQTERRTFLKTLGGATAALAAGPALEAGAQAPPRPAGAR
                     YMGDFAAPRLDKVRLAMIGVGARGSGHVRQMAAIEGVEVVAISDLYEDLAKRSEARVK
                     ALGHSPALYFGDANLWRKMLVEKKPDAVIIATPWEAHAPMGVEAMRAGAHVFIETPIA
                     TTIEDMWALVNTSEETGRHCMHMENVCYGREELLYLNMVRQGLIGEVIHGEAAYIHEL
                     RRQMRQVERGTGSWRTQHYIKRNGNLYPCHGLGPVAQYMSLARTEDTFGRLVSFSSPA
                     RARALYAKANFPPDHKWNQATFRAGDINTSIVTTALGRTIVVQWDENSPRPYSRHNLI
                     QGTKGILAGFPNRLAIEGVPELKSYHDWTEGEAWEQFVAQYEHPLFKRAGELAKKMGG
                     HGGMDFLMLYRIVECLRNGLPLDQNVYEGCFWSSVGPLSEKSVAAGGAPQDFPDFTRG
                     HWKTTKPLDIVA"
     gene            complement(3849..4946)
                     /locus_tag="PKK95_06905"
     CDS             complement(3849..4946)
                     /locus_tag="PKK95_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17977.1"
                     /translation="MPPLPRRLLVLLTATLLTAVCTAPVGRKPATYAETVGALSEPGG
                     FFNSNNLISNERSYLHVIPELRRARLDGGVYLGVGPDQNFTYIAQTRPSIAFIVDIRR
                     DNLLLHLLFRALFKLSDNRAEYLSLLFGRPEPKPRAAWKRASLDRIAEHIDLGPAPPE
                     AVTAMRARVDAAIERLGVPLSKADFETIDRFHRIFIESGLGLQFESAGRPPQRYYPTY
                     RELLLETDHDGRRWNYLANEEDFQFVRDLQRRGLIIPVVGDLGGRTAMAAVGRLLQQR
                     GAHLSVFYTSNVEYYLASRGVFDQFVTNLANLPRSERSVIVRAVFPSRYGWAPTTPGY
                     ISSSTVQRVDDLLSGVAAGRIRGYGDLINGQ"
     gene            complement(5003..5698)
                     /locus_tag="PKK95_06910"
     CDS             complement(5003..5698)
                     /locus_tag="PKK95_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOC17978.1"
                     /translation="MNGVPYDLLGFVTLGLLGGFGHCVGMCSPFVLFVSRRYHPVVRG
                     GDPGGTAGGSRRLAPLAPQGWYHAGRIVTYAALGALAGTVGGAANLAGNLVGLQRGAM
                     LVAGAVLAAWGLVALSDLASFDAGGRFFGRVAGRLKGRVPEHPFATGLFLGLLPCGFL
                     YSAVVAAVSRGGAVQGASALALFGLGTAPALLGVSVADELLARRRAFFNRLAQLFILV
                     MGGWFLWRGLFGG"
     gene            complement(5695..6909)
                     /locus_tag="PKK95_06915"
     CDS             complement(5695..6909)
                     /locus_tag="PKK95_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17979.1"
                     /translation="MTREHYRALPAVPGRLAPPRQVLVSSLAAVLAGSAALAWAFARG
                     EAQLAWSAYLIGAFFALGLGAFAVAWLSMLYLAGADWSVTMRRIPEAMTAWLIPGGLL
                     TMAVALGAHPLYHWSHADAVANDALLLHKSPFLNATAFTVLLGASLLLWAAFGARMVG
                     ASLRQDREGGHAATRANRTLSAIFLVVFAVTLSIVSFYLLLSLEAHWFSTMFAVLVFT
                     DIVQTGTAFVAVAAGLLVMSGTLRGFLNEHHLHSLGKMVFAATGFWAYIYFCQYMLIW
                     YANIPEETTYFLRRADHGWLPYFAILPVLKFVVPFLLLLPRDAKRNPRKLVPVAVLLL
                     FAQFWELYVMVGPALTHGVEAAHGHLPVVELAVTLGFLGLFTLVFGWALSRHDPVPLK
                     DPALLECLEYHS"
     gene            complement(6906..7562)
                     /locus_tag="PKK95_06920"
     CDS             complement(6906..7562)
                     /locus_tag="PKK95_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOC17980.1"
                     /translation="MRRRTVPSAAGTFRLLARPAAVLLLAALASACDVGLPAGRTPHR
                     EGNRLDMGDQPKLKPQRKDLFGARPTGTMEPQPGTLAVGEAPYPYAQNEADRAGAELV
                     NPLQATPAALAHGQFVYEQVCITCHGPKGAGDGHVTAVFPKPPSLMTQKVRDWPDGQL
                     FHRPMRGQNSMPSHARQVNAHDIWSVIHYIRQMQKAEPVAPASAPADTPDATSKGKAS
                     "
     gene            complement(7555..8106)
                     /locus_tag="PKK95_06925"
     CDS             complement(7555..8106)
                     /locus_tag="PKK95_06925"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOC17981.1"
                     /translation="MVPNSTKGVLGHFYEPADALAAAVKVRDAGWKHFDFLTPFPIHG
                     MEEAMGQKRSWLPWVTAVLALCGILFAQALQNYVMVWDWPMNFGGKPFAAWPAFIPIT
                     FEAMVFWAALGSAVVAVVAGKMDSVPQPPPMAVKTGATFDRFVLWISATDPKWNAAEA
                     ERFVRSLGADGVRLVEVAGGTNA"
     gene            complement(8110..9600)
                     /gene="nrfD"
                     /locus_tag="PKK95_06930"
     CDS             complement(8110..9600)
                     /gene="nrfD"
                     /locus_tag="PKK95_06930"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOC17982.1"
                     /translation="MSPYATVMDDGQLTGRPIVLPGAGPYGGDLEVAEPLVTGGKTWA
                     EVDQDICMHAERFPTRRWWIAFSVALAFLGLLVFSLTTLFYAGMGIAGVNQPVGWGSF
                     IINFVFWIGIGHAGTLISAVLYLFRQQWRTGINRAAEAMTLFAVACAGIFPIIHLGRP
                     WFAYWLVPYPNARGPLWVNFNSPLAWDIFAISTYGLVSLTFWYMGLLPDLATVRDRAT
                     HPWRRRLYNAMSFGWTGSHRAWRHYESGYMILAALATPLVFSVHTIVSFDFATSVMPG
                     WHTTIFPPYFVIGAIFSGFAMVITLMTFMRGYFGMKNYITLNHMEAMAKILMLTGMLV
                     GFAYSTEFFMAWYSGSEWEGFIFKNRAFGPYWWAYAIMFTCNAIVPQLFWWKPMRRHT
                     AVLFVVSILVNVGMWFERYVIVVTSLHRDFLPSSWGMYALTFWDYAILLGSFGLFFTL
                     FLLFVRVLPAVAISEVKGVMDPKVARGMPSPTPLPTAAGGGAFEEV"
     gene            complement(9602..>10719)
                     /locus_tag="PKK95_06935"
     CDS             complement(9602..>10719)
                     /locus_tag="PKK95_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC17983.1"
                     /translation="PLGLGRAACGVIGAGVGPNAFPLRRLLDGRLVSSGLPVSIRKAG
                     GREELAFAQGSDVIDRARRPLVPVTTLSAFEADARSGTEQQHGGPSAWPEHPYPNARW
                     AMAIDLSLCNGCGKCVLACQAENNVPVVGRRGMLDGREMSWIRIDRYYDAPKKDGGWD
                     AEVWDGPLEVVEEPRTLFQPMLCQHCENAPCETVCPFVATMHSEEGLNQQIYNRCVGT
                     RYCANNCPFKVRRYNFWEYSKAQESRFFRWLEPRIARNAALNARSPMQMKNNPEVTVR
                     SRGVMEKCSFCVQRIKAARSEATREGKPRDHFEDGAVVTACMEACPTGAIVFGDANAP
                     NSRVAALAAGPRAMKLLEALGVEPSISYLSRVRHDKA"
BASE COUNT         1621 a         3682 c         3800 g         1616 t
ORIGIN      
        1 cgcctgtggg tgcccaaggg cccagtggca caggcgtccc gcctgtgggg gctctcttcg
       61 accccgagga cgttgcctgg accgcgcccc tgctggccct ctgggtcagc ccgctggcga
      121 tcctggccgc ggtgcccggc gtggcgcagg tgttcccggc cttcgcgttc gtgctcgtcg
      181 atctgcgatg gtggtggacc gccgccattg ccgccgcaac gctcgttggc gcgtggcggc
      241 gggtgcgagg aacacctccc ggtgatacac cgcctcgcgg tgacacgctc ccgagtgggc
      301 gcctcgctgt tccgcgcctg ccgcccgcgc tggccctggc cgctctgtgc ggggcgtggg
      361 tcgtcctcgg cacgccccac ctccggttct cgccggtggt ccacggcgac gaggcgaagt
      421 accttcgcta ctgcgaaaac ttctaccagg ggcacgggct ggagatctcg agcatccggc
      481 cgatcgcgca gcttccgccc ggcttcgcgc cgagattcgg ccgcaacctc gagctggcgg
      541 cgcaggtgct tcccggcgag ctgagaaacc tcgctgccga tgcccgcgtc ttcctctccg
      601 acccatctca caagttcgcc aggtcggtga aggctcgcgg tttcgtgcga ggaaagaacg
      661 gcggcgtcta ccaggtgcac cagccgggcc tgtcggtgct gatgctcccg gcctatagcc
      721 tcgacaggtg gctttcggtg ccggggcgcc gttccccacg tcagtggccg ccacgcctgg
      781 tggccgtcaa cgcgttcttc ctctccctgt atctcgtgtg ggtcgtgctg gtctaccggc
      841 tcgcggtacg gctgacagga gacagccggg tggcggcgac ctcggcattg gcggtcggcg
      901 ccacgcttcc ggttgccgcg tttccgttcc agttctatcc cgaggccgcg gcgggcatcg
      961 tggtgatcgc ggttgccggg cacgtgttct tcccgcttcg ccgcacgccg gcggctgcgc
     1021 tcgcggccgg gttcgccacg ggctgcctgc cgtggctgca cgtgaggttc gttccgctcg
     1081 cggtcctgct cgcggccttc gccatgattg cggggcgccg cgaggggcgg cgggtcgcgg
     1141 ggttcgtctc aggcgctgcg attcccacgg ccttcctgct gctgtacgtc tatcgagtga
     1201 ccggcagcat cctgccgact tcgctgtggg cgggcgagtc gggcgctctg ccgctctcgg
     1261 ccctgaccgc ggtccccggc agcgtcgcct acctgctcga ccgccgctgg ggcttgctcg
     1321 cccacgcgcc catctacctg ctcgcgcttc ccggctggtg gctgctggcc aggcagcgcc
     1381 gcgacctcgc gtggttttgc gcgctgggct tcctggccgt gctgttgccc gccgcagcgc
     1441 acacgctgca cgccgccgcc accacgccgt gccgcctgat cgcggcggtg gtgccgctgg
     1501 cggcgctccc gcttgccgtc ctgctgacga ggtcgtctgg aaggcggtgg ttgaaggcag
     1561 ccttcgcggt gctgctcgcg ctgtcggtgc agaacgcgct cgcgtacaac tggtggcacg
     1621 tgaagccggt cgggtggctc gtcgaccaga gcttcagcgg ctggaaggtg aacctgttgt
     1681 tccccgcgcg cggcgcgaca ccctgggtga cggcgtcgaa cgggtggttg tacgtcgcct
     1741 gggtcctcgt cctggtggcg gcgtttgccg ccccgctgtg gttcgagcgg aggggaggag
     1801 agaccacggc ccgtgcgggg cgcgcgcagt tgcccgccgt cgcggcagcg ctggcgctcg
     1861 tcgcgctcct cgggacggcc gtcgcggcgg caaccgggtc gtgggaggag aacgactacc
     1921 ggcggccgaa cgccggcgcc gccgggcgtg ccgcggcact gctcctgcaa cccggcgaac
     1981 gccttcgcct ctcatccgaa cgcggccgca tcggggccga cgctgccctg gcccaactcg
     2041 ccaccgtgca tccctcgctg gccgggccgc caggcgggga ggcggcagag ccatcgcccc
     2101 ccgacgagga ggagaactcc agtgcgccac ggtgatcgca ctggtgattg actgacggtt
     2161 acgccacgat gtcgagcggc ttggtcgtct tccagtgtcc cctcgtgaag tcgggaaagt
     2221 cctgcggggc gccccccgcg gcaaccgact tctcgctgag ggggccgacc gacgaccaga
     2281 agcagccctc gtaaacgttc tgatccagcg gcagcccgtt ccgcaggcac tcgacgatgc
     2341 ggtacagcat caggaagtcc atgccgccgt ggccgcccat ctttttcgcc agttctcccg
     2401 cccgcttgaa gagcgggtgc tcatactgcg ccacgaactg ctcccacgcc tcgccctcgg
     2461 tccaatcgtg gtagctcttc aactccggca ccccctcgat ggccaggcgg ttcgggaacc
     2521 cggccaggat gcccttggtg ccctggatca ggttgtggcg cgagtagggc ctcggggagt
     2581 tctcgtccca ctgcaccacg atcgtccggc cgagtgccgt cgtcacgatc gaggtgttga
     2641 tgtcgccggc gcggaaggtg gcctggttcc acttgtggtc gggcggaaag ttcgccttgg
     2701 cgtagagggc acgtgccctg gcgggtgacg agaacgagac caggcggccg aaggtgtcct
     2761 cggtgcgcgc gaggctcatg tactgcgcga ccggcccgag accgtggcag gggtacaggt
     2821 tcccgttgcg cttgatgtag tgctgcgtgc gccacgagcc ggtgccgcgc tcgacctgcc
     2881 gcatctggcg gcgaagttcg tggatgtacg cggcctcgcc gtgaatgact tcaccgatga
     2941 gcccctgccg gaccatgttg aggtagagca gttcctcgcg tccgtagcac acgttctcca
     3001 tgtgcatgca gtggcgcccg gtttcctccg acgtgttgac cagcgcccac atgtcctcga
     3061 tcgtcgtcgc gatcggggtc tcgatgaaga cgtgggcgcc ggcccgcatc gcttcgaccc
     3121 ccatcggcgc gtgcgcttcc cagggcgtcg cgatgatcac ggcgtcgggc ttcttctcga
     3181 cgagcatctt ccgccacaag ttcgcgtcgc cgaagtacag cgccgggctg tgcccgagcg
     3241 ccttgaccct cgcctcggac cgcttggcca ggtcctcgta gaggtcggag atcgccacga
     3301 cctcgacccc ttcgattgcc gccatctgcc gcacgtggcc cgagccgcgc gccccgaccc
     3361 cgatcatcgc cagcctcacc ttgtcgagcc tgggcgcggc gaaatcgccc atgtaccgtg
     3421 cgccggcggg gcgcggcggg gcctgtgcgc cggcttccag cgccggcccg gcggccaggg
     3481 ccgcggtggc gccgccgagg gtcttcagga aggtgcggcg ttcggtctga gtcggcttct
     3541 tcttcataat ctcttgtcag cctcggagga atgtggcggt gcggcgcgcc gccggtcggc
     3601 ccaatcctac aacggcgggg aaccagtggc acaggcgtct cgcctgtggc ggccaagggg
     3661 cccagtggca caggcgtctc gcctgtgagg tccatgggct cagtggcaca ggcgtctcgc
     3721 ctgtggcggc caaggggcgc agtggcacag gcgtctcgcc tgtggcggcc aaggggccca
     3781 gtggcacagg cgtctcgcct gtgaggtcca tgggctcagt ggcacaggcg tctcgcctgt
     3841 gaggcacgct actgtccgtt gatcagatcg ccgtacccgc ggatccgccc ggcggccacc
     3901 ccgctcagca gatcgtccac gcgctgcacc gtcgacgagc tgatgtaccc cggtgtcgtc
     3961 ggagcccagc cgtagcggct cgggaaaacc gcccggacga tgacgctgcg ctcgctgcgc
     4021 ggaaggttcg ccaggttggt gacgaactgg tcgaagacgc cgcggctggc gaggtaatac
     4081 tcgacgttcg acgtgtagaa gaccgagagg tgcgccccgc gctgctggag gagccgtccc
     4141 acggcggcca tcgccgtccg gccacccagg tcgccgacca cggggatgat caggccgcgc
     4201 cgctgcaggt cgcgtacgaa ctggaagtct tcctcgttcg ccaggtagtt ccagcggcgg
     4261 ccgtcatgat cggtctcgag caacagttcc cggtaggtgg ggtagtagcg ctgcggcggg
     4321 cggccggccg actcgaactg cagcccgagc ccgctctcga tgaagatccg gtggaaacgg
     4381 tcgatcgttt cgaaatcggc cttcgagagc gggacgccga gccgctcgat cgccgcgtcc
     4441 acgcgggcgc gcattgccgt gaccgcttcg ggcggcgccg ggccgaggtc gatgtgctcg
     4501 gcgatgcgat cgaggctcgc gcgcttccac gcggcgcgcg gcttcggctc gggccgcccg
     4561 aagagcaggc tcaagtactc ggcgcggttg tcggacagct tgaagagcgc gcgaaaaagc
     4621 aggtgcagca gcaggttgtc gcggcggatg tcgacgatga aggcgatcga cggccgcgtc
     4681 tgcgcgatgt aggtgaagtt ctgatcgggg ccgacgccga ggtacacgcc cccgtcgagc
     4741 cgcgcgcggc gcagctcggg gatcacgtgc aggtacgacc gctcgttcga gatcaggttg
     4801 ttgctgttga agaagccgcc aggttccgag agggcgccga ccgtctcggc gtaggtggct
     4861 ggcttccggc cgacgggcgc cgtgcagacc gcggtcagca gcgtcgccgt cagcaggacg
     4921 aggaggcggc gcggcagggg gggcatgccc cagtataagc aagcgccggt gcactggtgc
     4981 cggctggcag cccgtcagtt cgtcacccgc cgaacaggcc tctccagagg aaccacccgc
     5041 ccatgacgag gatgaacagc tgcgcgaggc ggttgaagaa ggcgcggcgc cgggcgagca
     5101 gctcgtccgc caccgagacg ccgaggaggg ccggcgccgt gccgaggccg aagagggcga
     5161 gtgccgaggc tccttggacc gcgccgccgc gcgagacggc ggccaccacg gcggagtaga
     5221 ggaagccgca gggcagcagg cccaggaaca accccgtcgc gaacgggtgc tccggcaccc
     5281 gccccttcag gcggcccgcg acccggccga agaatcgtcc gccggcgtcg aacgacgcga
     5341 ggtcggacag cgcgacgagc ccccaggcgg ccagcaccgc gccggccacc agcatcgcgc
     5401 cccgctgcag gccgacgagg ttccccgcca ggttggccgc gcctccaacg gttcctgcca
     5461 gcgcgccaag tgccgcgtac gtgacgatgc ggcccgcgtg gtaccagccc tgcggcgcga
     5521 gcggcgcaag gcgccggctc ccgccagccg tcccgcccgg gtcgcctccg cgaacgaccg
     5581 ggtggtaccg gcgcgacacg aacagcacga acggcgagca catcccgacg cagtgcccga
     5641 agccgccgag caggccgagc gtcacgaagc cgagcaggtc gtagggaacg ccgttcacga
     5701 gtggtattcg aggcactcca ggagcgccgg gtccttcagc ggcacggggt cgtggcgtga
     5761 gagcgcccag ccgaagacca gcgtgaagag gcccaggaac ccgagcgtca cagccagctc
     5821 gacgaccggc aggtgaccat gggccgcctc caccccatgg gtcagcgcgg gccctaccat
     5881 gacgtacagc tcccagaact gcgcaaagag cagcagcacg gccacgggga cgagcttcct
     5941 cgggttgcgc ttggcgtccc tcggcagcag gagcaggaac ggcacgacga acttcagcac
     6001 cggcagaatt gcgaagtacg gcagccagcc gtggtcagcg cggcgcagga agtaggtggt
     6061 ctcctcgggg atgttcgcgt accagatgag catgtactgg cagaagtaga tgtacgccca
     6121 gaacccggtc gcggcgaaca ccatcttccc gaggctgtgc aggtggtgct cgttgaggaa
     6181 ccctctcagc gtgcccgaca tcaccagcag gccggccgcg acagcgacaa acgccgtccc
     6241 ggtctggacg atgtcggtga agacgagcac cgcgaacatc gtcgagaacc agtgcgcctc
     6301 gagcgagagc agcaggtaga agctgacgat gctcagcgtg acggcgaaga cgacgaggaa
     6361 gatggccgag agcgtgcggt tcgcgcgcgt ggcggcgtgc ccgccctcgc ggtcctgccg
     6421 cagcgaggcg cccaccatcc gcgccccgaa cgccgcccac aggagcaggc tcgcgcccag
     6481 cagcaccgtg aacgccgtcg cgttcaggaa gggcgacttg tgcagcagca gcgcgtcgtt
     6541 cgcgaccgcg tcggcgtgcg accagtgata cagcgggtgg gcgccgaggg cgacggccat
     6601 cgtcagcagc ccccccggga tcagccacgc ggtcatcgcc tcggggatcc gccgcatcgt
     6661 caccgaccag tcggccccgg ccaggtacag catcgacagc caggccacgg cgaacgcgcc
     6721 caggccgagc gcgaagaagg cgccgatcag gtacgcgctc caggcaagct gcgcctcgcc
     6781 gcgggcgaag gcccatgcca gggcggcgct ccccgcgagg acggccgcga gcgacgacac
     6841 caggacctgg cgcggcgggg ccaggcggcc cggcaccgcg ggaagggcgc gatagtgttc
     6901 ccgggtcatg aggccttccc cttcgaagtg gcgtcaggag tgtcggccgg agcggaggcg
     6961 ggggctactg gttccgcctt ctgcatctga cggatgtagt ggattaccga ccagatgtcg
     7021 tgggcgttca cctgccgcgc gtggctcggc atcgagttct gtccccgcat cgggcggtgg
     7081 aacagctgcc cgtccggcca gtcccgcacc ttctgcgtca tgaggcttgg cggcttcggg
     7141 aagaccgccg tgacgtggcc gtcgccggcg cccttcgggc cgtggcaggt gatgcacacc
     7201 tgctcgtaga cgaactgccc gtgcgcgagg gcagcgggcg tcgcctggag cgggttcacc
     7261 agttcggcgc cggcgcggtc ggcctcgttc tgcgcgtagg gatagggcgc ttcaccgacg
     7321 gcgagcgttc ccggctgcgg ctccatcgtg ccggtcgggc gggcgccgaa caggtccttg
     7381 cgctgcggct tgagcttcgg ctggtcgccc atgtcgaggc gattgccctc gcggtgaggc
     7441 gtccggccgg ccggcaggcc gacgtcgcag gccgacgcga gtgcggccag cagcagcacg
     7501 gcggccggcc gtgccagcag gcggaacgtg cccgccgccg aggggacggt ccttctacgc
     7561 attggtccct cccgccacct cgacgagcct gaccccgtcg gcgccgagcg agcggacgaa
     7621 gcgctccgcc tcggcggcgt tccacttggg gtcggtggcc gagatccaga ggacgaaccg
     7681 atcgaaggtc gccccggtct tcaccgccat cggcggcggc tgcggaaccg agtccatctt
     7741 cccggcgacg acggccacca cggcgctgcc gagggcggcc cagaagacca tcgcttcgaa
     7801 ggtgatcggg atgaaggccg gccacgcggc gaacggcttg cccccgaagt tcatcggcca
     7861 gtcccagacc atcacgtagt tctggagggc ctgggcgaac aggatgccgc acagcgcgag
     7921 gaccgcggtc acccagggaa gccacgaccg cttctggccc atcgcctcct ccatcccatg
     7981 gatggggaag ggagtcagga agtcgaagtg cttccatccg gcgtcgcgca ccttcacggc
     8041 cgcggccagc gcgtcggccg gctcgtagaa gtggccgagg acgcccttgg tcgagttcgg
     8101 caccatggct cacacctcct cgaacgcgcc gccgccggcg gcggttggca gcggcgtggg
     8161 gctgggcatg ccccgcgcga ccttcggatc catcacgccc ttcacctcgg agatcgcgac
     8221 cgcgggcagg acgcgcacga agaggaggaa gagggtgaag aagaggccga aggagcccag
     8281 caggatcgcg tagtcccaga acgtgagggc gtacatcccc caggacgagg gcaggaagtc
     8341 gcggtggagc gacgtgacga cgatgacgta gcgctcgaac cacatgccga cgttcaccag
     8401 gatggacacg acgaagagaa ccgcggtgtg ccgccgcatc ggcttccacc agaacagctg
     8461 cggcacgatc gcgttgcagg tgaacatgat cgcgtaggcc caccagtacg gcccgaacgc
     8521 ccggttcttg aagatgaagc cctcccactc gctgcccgag taccacgcca tgaagaactc
     8581 ggtcgagtag gcgaagccga cgagcatgcc cgtcagcatc aggatcttcg ccatcgcctc
     8641 catgtggttc agggtgatgt agttcttcat cccgaagtag ccgcgcatga aggtcatcag
     8701 cgtgatgacc atcgcgaacc ccgagaagat ggcgccgatg acgaagtagg gcgggaagat
     8761 cgtggtgtgc cagccgggca tcaccgaggt ggcgaagtcg aacgagacga tcgtgtgcac
     8821 cgagaagacc agcggcgtcg ccagcgcggc caggatcatg tagcccgact cgtagtggcg
     8881 ccacgcgcgg tgggagccgg tccagccgaa gctcatcgcg ttgtacagcc gccgcctcca
     8941 cgggtgggtg gcgcggtcgc ggaccgtggc caggtctggc agcagcccca tgtaccagaa
     9001 ggtgagggag acgaggccgt aggtggagat cgcgaagatg tcccaggcga gcggggagtt
     9061 gaagttcacc cacagcgggc cgcgcgcgtt ggggtaggga acgagccagt aggcgaacca
     9121 ggggcgcccg aggtggatga tcgggaagat gccggcgcag gcgaccgcga agagcgtcat
     9181 cgcctcggcc gcccggttga tgcccgtccg ccactgctgg cggaacaggt agaggacggc
     9241 cgagatcagc gtgcccgcgt ggccgatgcc gatccagaac acgaagttga tgatgaacga
     9301 gccccacccg accggctggt tgacgcccgc gatgcccatg ccggcgtaga agagcgtggt
     9361 cagcgagaag accagcaggc cgaggaacgc cagcgcgacg ctgaacgcga tccaccaccg
     9421 cctcgtgggg aaccgctcgg cgtgcatgca gatgtcctgg tcgacctcgg cccacgtctt
     9481 gccccccgtg acgagcggtt cggcgacctc gaggtcgccc ccgtaggggc cggcgcccgg
     9541 aagcacgatc ggccggccgg tcagctggcc gtcgtccatc acggtggcgt agggactcac
     9601 ctcatgcctt gtcatgacgc accctggaca ggtaggagat ggacggctcg acgccgagcg
     9661 cctcgaggag cttcatggcg cgtggcccgg cggcaagcgc cgccacgcgc gagttcgggg
     9721 cgttcgcgtc gccgaagacg atggcgccgg tcgggcaggc ctccatgcac gccgtgacga
     9781 cggccccgtc ctcgaagtga tccctcggct ttccctcgcg cgtcgcctcc gaccgcgccg
     9841 ccttgatgcg ctggacgcag aaggagcact tctccatcac gccgcggctg cggacggtga
     9901 cctcggggtt gttcttcatc tgcatcgggc tgcgggcgtt gagcgccgcg ttccgcgcga
     9961 tccgcggctc gagccagcgg aagaagcggc tctcctgcgc cttgctgtac tcccagaagt
    10021 tgtaccgccg caccttgaag gggcagttgt tcgcgcagta ccgggtgccc acgcagcggt
    10081 tgtagatctg ctggttcagt ccctcctcgc tgtgcatcgt ggcgacgaag gggcagaccg
    10141 tctcgcacgg cgcgttctcg cagtgctgac agagcattgg ctggaagagc gtgcggggct
    10201 cttccaccac ctcgagcggg ccgtcccaga cctcggcgtc ccacccgccg tccttcttcg
    10261 gcgcgtcgta gtagcggtcg atccggatcc acgacatctc ccgcccgtcc agcatgcccc
    10321 gccgccccac gaccgggacg ttgttctcgg cctggcaggc gagcacgcac ttgccgcagc
    10381 cgttgcacag gctgaggtcg atcgccatcg cccaccgggc gttcgggtag gggtgctccg
    10441 gccaggccga cggccccccg tgctgctgct cggtgcccga gcgagcgtcg gcttcgaagg
    10501 ccgacagcgt ggtcacgggt accagcggcc gccgcgcccg atcgatcacg tccgagccct
    10561 gggcaaaggc cagttcctcg cggccgccgg ccttgcggat cgagaccggc agtcccgacg
    10621 agaccaggcg gccgtcgagc aggcgccgca gcgggaaggc gttcgggccg acgccggcgc
    10681 cgatgacccc gcaggctgcg cggcccagcc cgaggggaa
//
LOCUS       DAOPQX010000093        10624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7103_length_10624_cov_4.579241, whole genome shotgun sequence.
ACCESSION   DAOPQX010000093 DAOPQX010000000
VERSION     DAOPQX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7103_length_10624_cov_4.579241"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2958)
                     /gene="nifJ"
                     /locus_tag="PKK95_06940"
     CDS             complement(<1..2958)
                     /gene="nifJ"
                     /locus_tag="PKK95_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOC17984.1"
                     /translation="MTRPRITIDGNEAAASVAHRTNEVIAIYPITPSSNMGEWADEWS
                     AKGRKNIWGTIPQVTEMQSEAGAAGAVHGALQAGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSFCMHVSARTVAAHALSIFGDHSDVMACRQTGFALLASGSVQEAHDFAAIGQRAT
                     LRSRVPFLHFFDGFRTSHEVSKIEQLTDDDLRQLVTPDLVEAHRRRALTPDAPVMRGT
                     AQNPDAFFQAREAINPYYLVAPEIVQDTMDEFGKLVGRHYHLFDYVGHPEAERVIVMM
                     GSGAEVTHELVEWMVARGEKVGLIKVRLYRPFSAAHFLAALPATARRIAVLDRTKEPG
                     GLGEPLYLDVVTALRQEQTNGAARPIEPLVVGGRYGLSSKDYTPAQAKAVFDELAAEN
                     PKRQFTVGIVDDVTHLSLPVDPEFDIEPDDVVRAVFFGLGADGTVGANKNSIKIIGEE
                     TDNYGNGYFVYDSKKSGAITISHLRFGPRPIRSSYLVKKANFVACHQYVFLDKYDVLQ
                     YAQDGSVFLLNAPLPADRVWADLPVEVQQQIVDKRLQFYVIDAYEVAKATGMGTRINT
                     IMQTCFFAISGVLPREEAITQIKKAIKKTYGKRGEEVVRKNFAAVDQTLEHLHRVEVP
                     AAVTDGRRRPPLVSEQAPEFVQRVTATMMANRGDLLPVSAFPVDGTWPTDTARWEKRN
                     IALDIPVWDAKICIQCNKCAMVCPHAAIRAKVYDSDLLKDAPETFKSIDFKGGEFKGM
                     KYTIQVAPEDCTGCGICVSVCPAKDKANPKHKSIDMMPQRPIREAEAKNYEFFLNLPE
                     VDRSRVKTDVKGSQFFQPLFEYSGACAACGETPYVKLMTQLFGDRLLVANATGCSSIY
                     GGNLPTAPYAKNRDGRGPAWSNSLFEDNAEFGFGFRLAIDKKQEQAQELLRALAPQIG
                     EGLVEALLGADQSTEAGIAAQRERIAALGQKLAGLDSAEARWLATIADALVKKSVWIF
                     GGDGWAYDIGYGGL"
     gene            3349..6072
                     /locus_tag="PKK95_06945"
     CDS             3349..6072
                     /locus_tag="PKK95_06945"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HOC17985.1"
                     /translation="MNRLLALADAGQSVWIDFIRRALLDSGELERLVREDGVAGVTSN
                     PTIFEKAIGGSADYDAQIRRVLSEEPGIDPHELFERVALDDIRAACDVLRPVFERTGG
                     ADGFVSFEVPPSLADDTAATVSQAQRLWRAIDRPNAMIKVPATPAGIPAIEELIAAGV
                     NVNVTLMFSLDHYERVAQAYIRGLHRRRDLAGAASVASFFVSRVDSVVDKALEKIGTP
                     EALALRGRAAIANSRLVYRRFREIFDGEPFAALREKGARPQRPLWASTSTKNPDYRDV
                     VYVEELVAPRTVNTVPPATLDAFRDHGEVRGETAAEAPEAARQLLDELARLGIDLDAI
                     TERLQREGVASFAKSYDDLIAALRAKSRQLFVDRVCRQDLALGSAKPAVEERLAAWER
                     EGLMGRIRAKDHTVWSSTPVPELTDRLGWLTLPREMGAHAAELASLAAEIRREKIEQV
                     VLLGIGGSSLAPEVIHATFGRREGHPPLAVLDSTHPAAVQSLAGQLTPGKVLFVVASK
                     SGTTVEPNALFHYWWKWAGASGRCFVAITDPGTPLEGLARERGFRRVFHGTPEVGGRY
                     AALSLFGLVPAALTGVDVSTLIQDARWMTDVCLAGHPEPVNPALQLGAAIAELARAGR
                     DKLTFLVDPPFGALPAWLEQLIAESTGKNGTGIVPIADEPSLPVEKYGPDRVFVAIQT
                     PAGGAAGRERASALEAAGHPVVRISLDSPQNLGQEFFRWELAVAAAGAALGVQPFDQP
                     DVQLAKDMARRAMADPAAISGAALQPCPASRANEVIDEWLASGRPGDYVALQAFLAPS
                     AELTALLQELRGALGARSGLATTLGYGPRFLHSTGQLHKGGPNTGLFLQLIDDPADDV
                     AVPDLGFTFGQLLRAQADGDAAALRERGRRLLRVQLGREPLKALREMAGKG"
     gene            complement(6074..6502)
                     /locus_tag="PKK95_06950"
     CDS             complement(6074..6502)
                     /locus_tag="PKK95_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17986.1"
                     /translation="MDTTQMIGRPGRLRGEQRAHARIDVEAAGRLVWRDGRGTARFNS
                     VVIRDVSETGAYVESVSGAPIPLYRLVTLQAEPTPSSESLPSFLRRGKVLSAVYRVSP
                     TRPATGAPAGYGLRLLLEPRLRAVPAPAPSAEAVLAEATA"
     gene            complement(6736..9666)
                     /locus_tag="PKK95_06955"
     CDS             complement(6736..9666)
                     /locus_tag="PKK95_06955"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC17987.1"
                     /translation="MPAQPLAATMARAAAALQRGRAAEAIDWLAQATALPALRREDEL
                     DIRCALAEAHLQRDDVQQAAAVLGRAPDVLRERIPPSTLSALWRLHGRLAAVRGDQSR
                     AIGLLGRALKQAELALDSRAIGLAHYELAVCYRQLGDPAIVREEIAKAVPALHAAGDR
                     RHVALAHSLAGISFAQSGRSDEAMAAFRQAEQLAAAAQAEDVLALVCGNQANVELMRH
                     RHEQALALAERAVALHEHAGSLHGLAVALATLGQICVRLGDLARAERALRRALEVRTP
                     VQARETTGAVYDTLAQIHLIRGRYEEADGWLARAGEAYGTYGEQTSRWYDWSLRLLAA
                     RLALRRGDREEAIERASDVSATPGAPPADVMQAELLACEALLAGQQLDAAERRLAVVS
                     ARLDPRLMPGVWGEYLRLRAGVHLHRGQTTDAYHDLAQSASVFDLLGERYQGALSRLA
                     LGRLVSRVGARSAADRHLREALAVFEALGAEPDLAAARSEAAPRPASTTGEYLAAPGE
                     ADDVIVRRLVDAAVMPDLLALETASALLEAAGADCAAVFLQSPSRDVRVIASAGCDAD
                     AALALARSVGRGAAYGSGEMRTESLGHEGDDLRLGVVASSRAIGVITARRLRMIAAVA
                     RQGFELCGSRDRAAQPAERAALRSLDTILPGFVCASAAMARVVEQIQRLQGNDLTVLV
                     TGESGTGKELVARAIHAGSLRSGGMFLPYNCTTTTRELADSQLFGHRRGAFTGAVNDQ
                     AGLIRSAAGGTLFLDEIGDLPLEVQPKLLRFLEQGEVLPIGETRPQRVDVRVVAATNA
                     DLEQRVAEGKFREDLYYRLTVLRIHVPPLRERREEIPHLCALFLREACERLNKPDVQL
                     SRDALDLFSQHWWPGNVRQLRNEIQRAVALGTAGGVVGIEHLSPGFSALREEARPAAA
                     TLPVLRPDVTLAAAVDDLERSMIRAALDRSDGNISEAARTLGLTRRGLYLKMRRLGLE
                     GAPI"
     gene            9953..>10624
                     /locus_tag="PKK95_06960"
     CDS             9953..>10624
                     /locus_tag="PKK95_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17988.1"
                     /translation="MVSRTRLFAAGLAAAGLMALQSTPAAQSSTAGQSSSSSSQQSSM
                     SSQNQSPSYHLQQAERVLDEAWRTYGEGGSGSSSMSGSGTTGSTSGSTTGSTAGSTSG
                     TESGQSASSSSDYKGQLRDIRDDFQSLKKAWEQSKGSSTSGTTGSTSGTTGTASQSGS
                     QSWMTYYTQINQSLDRLNVPKSPAGASGTSGSSMSGTSGSTSGTGSTSGSTSGSSSTY
                     GQSGSG"
BASE COUNT         1513 a         3781 c         3781 g         1549 t
ORIGIN      
        1 gaggccgccg tagccgatgt cgtaggccca gccgtcaccg ccgaaaatcc agacgctctt
       61 cttcaccagg gcgtcggcga tcgtggcgag ccagcgcgcc tcggccgagt cgaggcccgc
      121 cagcttctgg ccgagggccg cgatccgctc gcgctgggcg gcgatgccgg cctcggtcga
      181 ctggtcggcc ccgagcagcg cctcgaccag cccctcgcct atctgcgggg cgagggcgcg
      241 cagcagctcc tgcgcctgtt cctgtttctt gtcgatggcg aggcggaagc cgaagccgaa
      301 ctccgcgttg tcctcgaaca gcgagttcga ccaggccggc ccgcggccgt cgcggttctt
      361 cgcgtacggc gccgtgggca ggttgccgcc gtagatcgac gagcagccgg tggcgttggc
      421 gaccagcagg cggtcgccga acagctgggt catcagcttg acgtacgggg tctcgccgca
      481 cgccgcgcac gcccccgagt actcgaagag cggctggaag aactgcgagc ccttgacgtc
      541 ggtcttcacc cggctgcggt ccacctcggg caggttcagg aagaactcgt agttcttcgc
      601 ttccgcctcg cggatggggc gctgcggcat catgtcgatc gacttgtgct tcgggttcgc
      661 cttgtccttg gcggggcaca ccgacacgca gatgccgcag ccggtgcagt cctcgggcgc
      721 cacctggatg gtgtacttca tccccttgaa ctcgcccccc ttgaagtcga tcgacttgaa
      781 ggtctcgggc gcatccttga gcaggtcgga gtcgtacacc ttggcgcgga tggcggcgtg
      841 cgggcagacc atcgcgcact tgttgcactg gatgcagatc ttggcgtccc acaccgggat
      901 gtcgagcgcg atgttccgct tctcccaccg cgcggtgtcg gtcggccagg tgccgtccac
      961 cgggaacgcg ctgaccggca gcaggtcgcc gcggttggcc atcatcgtcg ccgtcacgcg
     1021 ctgcacgaac tcgggggcct gctcgctgac cagcggcggc cgccggcggc cgtcggtcac
     1081 cgcggcgggc acctcgaccc ggtgcaggtg ctcgagcgtc tggtcgacgg cggcgaagtt
     1141 cttcctgacg acctcctcgc cgcgcttgcc gtacgtcttc ttgatcgcct tcttgatctg
     1201 ggtaatcgcc tcctcgcggg gcagcacgcc cgagatcgcg aagaagcagg tctgcatgat
     1261 cgtgttgatg cgcgtgccca tcccggtggc cttcgccacc tcgtaggcgt cgatcacgta
     1321 gaactgcagg cgcttgtcga cgatctgctg ctgcacctcg accggcaggt cggcccagac
     1381 ccggtcggcg ggcagcgggg cgttcaacag gaacaccgag ccgtcctgcg cgtactgcag
     1441 cacgtcgtac ttgtcgagga agacgtactg gtggcacgcg acgaagttcg ccttcttcac
     1501 gaggtaggac gaccggatcg gccgcggccc gaagcgcagg tgcgagatcg tgatcgcgcc
     1561 cgacttcttc gagtcgtaca cgaagtagcc gttcccgtag ttgtcggtct cctcgccgat
     1621 gatcttgatc gagttcttgt tggcgccgac cgtgccgtcg gcccccaggc cgaagaacac
     1681 cgcccgcacc acgtcgtcgg gctcgatgtc gaactcgggg tcgaccggga gcgagaggtg
     1741 ggtgacgtcg tccacgatgc cgaccgtgaa ctgccgcttc gggttctcgg cggccagctc
     1801 gtcgaagacg gccttcgcct gcgcgggcgt gtagtccttc gaggagaggc cgtagcggcc
     1861 gcccaccacc agcggctcga tcggccgcgc ggcgccgttg gtctgctcct ggcgcagggc
     1921 ggtgacgacg tcgaggtaca acggctcgcc gaggccgccc ggctccttgg tgcggtcgag
     1981 gaccgcgatc cgccgcgcgg tggccggcag cgccgccagg aagtgcgccg ccgagaacgg
     2041 gcggtagagg cggaccttga tcagcccaac cttctcaccg cgcgccacca tccactccac
     2101 cagctcgtgc gtcacctcgg cgcccgagcc catcatcacg atgacccgct ccgcctcggg
     2161 gtggccgacg tagtcgaaga ggtggtagtg gcgccccacc agcttcccga actcgtccat
     2221 cgtgtcctgc acgatttcgg gcgccacgag gtagtagggg ttgatcgctt cgcgcgcctg
     2281 gaagaaggcg tcggggttct gcgccgtgcc gcgcatcacg ggggcatcgg gcgtgagcgc
     2341 ccggcggcgg tgcgcctcga ccaggtcggg cgtgacgagc tgccggagat cgtcgtcggt
     2401 cagctgctcg atcttcgaca cctcgtgcga ggtgcggaag ccgtcgaaga agtgcaggaa
     2461 cggcacgcgc gaccgcagcg tcgcgcgctg gccgatcgcc gcgaagtcgt gcgcctcctg
     2521 gaccgagccc gaggcgagca gcgcgaagcc cgtctggcgg caggccatga cgtccgagtg
     2581 gtcgccgaag atcgacagcg cgtgggcggc caccgtgcgg gccgacacgt gcatgcagaa
     2641 ggaggtgagc tcccccgcga tcttgtacat attggggatc atcagcagca gcccctgcga
     2701 cgccgtgaag gtcgtcgtca gggcgccggc ctgcagcgcc ccgtgcacgg cgccggccgc
     2761 gccggcctcc gactgcatct cggtcacctg cgggatggtc ccccagatgt tcttcctgcc
     2821 cttcgccgac cactcgtccg cccactcgcc catgttcgac gagggagtga tggggtagat
     2881 ggcgatgacc tcgttcgtcc ggtgggcgac cgaggcggca gcctcgtttc cgtcgatcgt
     2941 gatgcgagga cgtgtcatat gagtgctttc tccgctaggc gaccgcgtgg gccggatggt
     3001 cgggccgagg tgcgcgaatc gacccggcag tgggccacag caaccttcgt gctgccgctg
     3061 aaggcgacga aatccttggc atttcgtgcc tcagcggttg attccgcaga tcgagcgccg
     3121 gaaatccggc agtcccggct cgtccgtgtt cacggtgcag tacgggggcg cgcagcggcc
     3181 cggctgcgac gttcgtctac aagctcgagg tgaaggaggt agagacgagg gcacaccgta
     3241 gggcgtgccc tgcactgcac aacctactaa tattagatcg ttccacggcc cggtgcgaac
     3301 ctttccgcac ctggctcgat gcggtccatt tcccatcagg agacgcccgt gaaccgcctg
     3361 ctcgcactcg ccgacgccgg tcaatcggtc tggatcgact tcattcgcag ggccctgctc
     3421 gacagcggcg agctcgagcg gctcgtccgg gaggacggcg tcgccggggt gaccagcaac
     3481 ccgaccatct tcgagaaagc gatcggcggc agcgccgact acgacgcgca gatccggcgg
     3541 gtgctgtccg aggagcccgg aatcgacccg cacgaactgt tcgagcgcgt ggccctcgac
     3601 gacatccgtg cggcctgcga cgtactgcgg ccggtcttcg agcggacggg cggggccgac
     3661 ggcttcgtga gcttcgaggt gcctccctcg ctggccgacg acaccgccgc caccgtctcg
     3721 caggcccagc ggctctggcg ggccatcgac cggccgaacg cgatgatcaa ggtgccggcg
     3781 acgccggccg gcatcccggc catcgaggag ctgatcgccg cgggcgtgaa cgtgaacgtc
     3841 acgctgatgt tctcgctcga ccactacgag cgcgtcgccc aggcgtacat ccgcggcctg
     3901 caccgccgcc gcgatctcgc gggcgccgcg tcggtcgcgt cgttcttcgt cagccgcgtg
     3961 gactcggtcg tggacaaggc gctcgagaag atcggcacac ccgaggcgct cgcgctccgc
     4021 ggccgcgcgg cgattgccaa cagccgcctc gtctaccggc gcttccgcga gatcttcgac
     4081 ggcgagccgt tcgccgcgct gcgggagaaa ggcgcgcgcc cgcagcggcc gctctgggcg
     4141 agcaccagca cgaagaaccc cgactaccgc gacgtcgtct acgtggagga gctcgtcgcg
     4201 ccccgtacgg tgaataccgt tccgcccgcg acgctcgacg ccttccgcga tcacggagag
     4261 gtgcgcgggg agactgccgc cgaggcgccc gaggccgcgc ggcagctgct cgacgagctg
     4321 gccaggctgg ggatcgacct cgacgcaatc accgagcgcc tgcagcgcga aggcgtcgcc
     4381 tcgttcgcca agtcctacga cgacctgatc gcggcgctcc gtgccaagtc gcgccagctc
     4441 ttcgtggatc gcgtctgccg gcaggacctg gcgctcggga gcgccaagcc cgccgtcgaa
     4501 gagcgcctcg cggcctggga gcgcgaaggg ctgatgggcc gcatccgcgc gaaggaccac
     4561 accgtctggt cgagcacccc ggtgcccgaa ctgacggatc ggctcggctg gctgacgctc
     4621 ccccgggaga tgggggcgca cgccgccgag cttgcctccc tggccgccga aatccggcgc
     4681 gagaagatcg agcaggtggt gctgctcggc atcggcggct cgagcctcgc gcccgaggtc
     4741 atccatgcca ccttcggccg ccgcgagggc catccccccc tcgccgtcct cgacagcacg
     4801 caccccgccg cggtgcagag cctggccggg caactgaccc ccggcaaggt gctgttcgtc
     4861 gtcgccagca agtcgggcac gacggtcgag cccaacgcgt tgttccatta ctggtggaag
     4921 tgggcgggcg cgtcgggccg ctgcttcgtg gcgatcacgg acccgggcac gcccctcgaa
     4981 gggctggcgc gggaacgcgg gttccgccgc gtgttccacg gcacgcccga ggtcgggggg
     5041 cgctacgccg cgctcagcct gttcggcctg gtgcctgccg cgctgaccgg tgtggacgtc
     5101 tcgaccctga tccaggatgc gcggtggatg accgacgtct gcctggccgg gcaccccgag
     5161 ccggtcaacc ccgccctcca gctcggtgcc gccatcgcgg agctggccag ggccgggcgc
     5221 gacaagctga ccttcctggt cgatccgccc ttcggcgcgc ttcccgcctg gctcgagcag
     5281 ttgattgccg agagcacggg gaagaacggg accgggatcg tgccgattgc cgacgagccg
     5341 tctcttcccg tcgagaagta cgggcccgat cgcgtcttcg tcgccatcca gaccccggcg
     5401 ggcggcgcgg ccggccgcga acgggcgtcg gcactcgagg cggccgggca cccggtcgtc
     5461 cggatcagcc tcgacagtcc gcagaacctc ggccaggagt tcttccgctg ggagcttgcc
     5521 gtcgcagcgg ccggggccgc cctcggcgtc cagccgttcg accagcccga cgtgcagctc
     5581 gcaaaggaca tggcccggcg tgcgatggcc gaccccgctg ccatctcggg cgcggcactg
     5641 cagccgtgcc cggcgtcgcg ggcgaacgag gtgatcgacg agtggctcgc ttcgggccgg
     5701 cccggcgact acgtcgccct gcaggcgttc ctcgcgccca gtgccgagct caccgctctc
     5761 ctgcaggaac tgcggggagc gctcggggcg cggagcggtc tggccaccac gctcggctac
     5821 ggtccgcgct tcctgcactc gaccggccag ctgcacaagg gagggcccaa caccgggttg
     5881 ttcctccagt tgatcgacga cccggccgac gatgtggcgg tgcccgatct cgggttcacc
     5941 ttcggccagc tgctgcgggc gcaggccgac ggcgacgcgg cggcgctccg cgagcgcggg
     6001 cggcggctgt tgcgggtgca gctgggtcgc gagcccttga aggcgctgcg agagatggca
     6061 gggaaggggt aggctaggcg gtcgcttcgg ccagcacggc ctcggcgctg ggggcgggcg
     6121 ccggcacggc gcgaagccgc ggctcgagca gcagccgcag gccgtagcct gccggcgcgc
     6181 cggtcgccgg ccgcgtcggg ctcacccggt agaccgccga gagcaccttg ccccggcgca
     6241 ggaacgaggg cagcgactcg gaggaaggcg tcggctcggc ctgcagggtc accaggcgat
     6301 agagggggat gggcgcgccg ctcacgcttt cgacgtaggc gcccgtctcg ctgacgtcgc
     6361 ggatcaccac gctgttgaag cgcgcagttc cgcgtccgtc ccgccacacg agccggcccg
     6421 cggcctcgac gtcgatccgt gcgtgcgccc gctgttcacc tcgaagcctg cccggccgcc
     6481 cgatcatctg cgtggtgtcc atcgtaggtc cccttcgcgc ccctgtctcc tggcccctag
     6541 cccttcaact cctggtccgc ccggccagac cgttggccgg tccggctcaa tccatcccaa
     6601 ccccttgtat ttaaaggatg ttgctgcgaa gggcggctct cgggacgccg cccggggtgg
     6661 atacgaagta tccattcatc cccacgactg gataccgagt gatcgggctt gaagcgggat
     6721 tcagggccaa gcgggctaga ttggcgcgcc ctcgagaccc agtcgtctca ttttgaggta
     6781 caggccgcgg cgggtgaggc ccagagtacg ggcggcctcg gagatgttgc catcgctgcg
     6841 gtcgagcgcg gcgcggatca tcgaccgctc caggtcgtcc acggcggccg ccagggtgac
     6901 gtcgggccgg aggacgggaa gggtggcggc ggccggccgc gcctcttcgc gcagcgccga
     6961 gaagcccggc gagaggtgtt cgatgccgac gacgccgccg gccgtgccaa gggcgacagc
     7021 ccgctggatc tcgttgcgca gttgccgcac gttgcccggc caccagtgct gcgagaagag
     7081 gtcgagcgcg tcgcggctca actgcacgtc gggcttgttc agccgctcgc aggcctcgcg
     7141 caggaacagc gcgcacaggt gggggatctc ctcgcgccgc tcgcgcagcg gcgggacgtg
     7201 gatgcggagc accgtcaggc ggtagtagag gtcttcgcgg aacttgccct cggcgacgcg
     7261 ctgctcgagg tcggcgttgg tggccgccac cacgcgcacg tcgacgcgct gaggccgcgt
     7321 ctcgccgatc ggcaacacct cgccctgctc gaggaaccgc agcagcttcg gctgcacctc
     7381 cagggggagg tcgccgatct cgtcgaggaa caaggtgccg ccggccgccg accgaatgag
     7441 gcccgcctgg tcgttgacgg cgccggtgaa cgcgccgcgg cggtgcccga acagctggct
     7501 gtcggccagc tctcgcgtcg tggtggtgca gttgtacggc aggaacatcc cgccgctgcg
     7561 cagcgagccg gcgtggatcg cgcgggcgac cagttccttg ccggtgccgc tctcgccggt
     7621 gacgaggacg gtcagatcat tgccctgcag tcgctggatc tgctcgacca cccgcgccat
     7681 cgcggcgctc gcgcagacga accccggcag gatcgtgtcg agcgatcgca gcgccgcgcg
     7741 ctcggccggc tgcgcggcac ggtcgcggct gccgcacagc tcgaatccct gtcgcgccac
     7801 cgcggcgatc atcctcaggc gccgcgccgt gatcaccccg atcgcgcgcg acgaggcgac
     7861 gacgcccagg cgcaggtcgt cgccctcgtg gccgagcgac tcggtgcgca tctcgccgct
     7921 gccgtaggcg gcgccccgcc ccaccgaccg cgcgagggcg agcgccgcgt cggcgtcgca
     7981 gcccgcgctc gcgatcacgc gaacgtcgcg cgagggggac tggaggaaca ccgcggcgca
     8041 gtccgcgccc gctgcctcga gcagcgccga cgccgtttcg agggccagca ggtcgggcat
     8101 caccgccgcg tccaccaggc gccgcacgat cacgtcgtcg gcctcgcccg gggcggcgag
     8161 gtactcgccg gtcgtcgacg ctggccgcgg tgcagcctcc gagcgggcgg cggcgaggtc
     8221 gggttccgcg ccgagggcct cgaacacggc gagcgcctcg cgcaggtgcc ggtcggcggc
     8281 cgaccgcgcg ccgacccgcg agacgaggcg ccccagcgcc agccggctca gcgccccctg
     8341 gtagcgctcg ccgagcaggt cgaacacgct cgcactctgc gcgaggtcgt ggtacgcgtc
     8401 ggtcgtctgc ccccggtgca ggtgcacgcc ggcgcgaagc cgcaggtact cgccccacac
     8461 gccgggcatc aggcgggggt cgagccgcgc cgagacgacc gccagtcggc gttcggctgc
     8521 gtcgagctgc tgcccggcga ggagggcttc gcaggcgagc aactcggcct gcatgacgtc
     8581 ggcgggcggc gcgccgggcg ttgccgacac gtcggaggcg cgctcgatgg cctcctcgcg
     8641 gtcgccgcgg cgcagcgcga ggcgcgcggc cagcaggcgc agcgaccagt cgtaccagcg
     8701 gctggtctgc tcgccgtacg tcccgtacgc ctcgccggcg cgggccagcc acccgtcggc
     8761 ctcctcgtag cgtcccctga tcaggtgaat ctgcgccagc gtgtcgtaca ccgcgccggt
     8821 cgtctcgcgc gcctgcaccg gggtgcgcac ctcgagcgcc cgccgcagcg cccgctcggc
     8881 ccgggcgagg tcgccgagcc gcacgcaaat ctgcccgagc gtcgcgagcg ccaccgcaag
     8941 gccgtgcagc gagccggcgt gctcgtggag ggcgacggcg cgctcggcca gcgccagcgc
     9001 ctgctcgtgg cggtggcgca tcaactccac gttggcctgg ttgccgcaca ccagcgcgag
     9061 cacgtcctcc gcctgtgccg ccgccgccag ctgctcggcc tgccggaacg ccgccatcgc
     9121 ctcgtcgctg cgacccgact gcgcgaagct gatccccgcg agcgagtggg cgagcgcgac
     9181 gtgacgccga tcaccggccg cgtgcagcgc gggcaccgcc ttggcgatct cctcgcgcac
     9241 gatggccgga tcgccgagct ggcggtagca gaccgcgagc tcgtagtggg cgaggccgat
     9301 cgcgcgcgag tcgagcgcga gctccgcctg cttcagcgcg cgccccagca ggccgatcgc
     9361 ccgcgactgg tcaccgcgga cggccgcaag ccggccgtgc aggcgccaga gggccgagag
     9421 ggtggaaggg gggatgcgct cgcgcaggac gtcgggcgcg cgcccgagca ccgccgcggc
     9481 ctgctgcacg tcatcgcgct gcaggtgcgc ctcggccagt gcgcaccgga tgtcgagctc
     9541 gtcctcgcgc cggagcgcgg gcagggcggt ggcctgcgca agccagtcga tggcctcggc
     9601 ggctcggccg cgctgcagcg ccgccgcggc gcgcgccatg gtcgcggcca atggctgggc
     9661 aggcacggcg gagagtatag cgcgcctttc caaccgtgaa tcgcgcgagg tgtctgcaat
     9721 ggcgcactgc agatcgccgc ggcgcccgcc tcgcgggccc ttgcgtgtca agggcttccg
     9781 gggcgatccc tgtctgtcga gctctgcgtg gcacccgcct tgctccggac atcacgggca
     9841 aatcagtgag agaaggcgcg gagcgtaccc ccacggtgac gccgcgccgc gcccggcata
     9901 aatggccctc atggtggccg ggcttggaac ggaacacagg aggatacgat cgatggtttc
     9961 acgaacgcgt ctcttcgcgg ctggactggc agccgcgggc ctgatggccc tgcagagcac
    10021 accggcggcc cagagcagca cggcggggca gtcgtcctcg tcgagctcgc agcagtcgtc
    10081 gatgtcgagc cagaaccaga gccccagcta ccacctgcag caggcggagc gggtgctcga
    10141 cgaagcctgg cgcacgtacg gggaaggcgg cagcggctct tcctcgatga gcggttcggg
    10201 caccaccggt agcacgagcg gatcgaccac gggatcgacg gccggttcga cgagcggcac
    10261 cgagagcggt cagtccgcct cgtcatcgtc cgactacaag gggcagctcc gcgatatccg
    10321 cgacgacttc cagtcgctca agaaggcgtg ggagcagtcg aaggggagca gcaccagcgg
    10381 cacgaccggc agcaccagcg gcaccacggg gacggccagc cagagcgggt cgcagtcgtg
    10441 gatgacgtat tacacgcaga tcaaccagtc gctcgaccgc ctcaacgtgc cgaagagccc
    10501 cgcgggcgcc tcgggcacgt cgggctcgtc gatgagcggt acctccggca gcaccagcgg
    10561 aacggggagc acttccggca gcacgtcggg cagctcctcg acctacgggc agtccggaag
    10621 cggc
//
LOCUS       DAOPQX010000094        10607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7117_length_10607_cov_5.084060, whole genome shotgun sequence.
ACCESSION   DAOPQX010000094 DAOPQX010000000
VERSION     DAOPQX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7117_length_10607_cov_5.084060"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKK95_06965"
     CDS             <1..512
                     /locus_tag="PKK95_06965"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HOC17989.1"
                     /translation="ISGGTATLDAEERQVDGKVELEGRIAEIPVGATDTFYVGETMVT
                     VTGATVVRHGETPVAFATLKVGDRVHVRGVPGTAPNSIAAELVIVQNTNAKVPANLKG
                     KISALVDGTACPALEFKLSGWTVATSGSTTYSKGKSCVDLAAGTGVHVKGTVVAGKVA
                     AGSVQFDKK"
     gene            complement(568..1308)
                     /locus_tag="PKK95_06970"
     CDS             complement(568..1308)
                     /locus_tag="PKK95_06970"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOC17990.1"
                     /translation="MDWILNPEIWVALLTLTALEIVLGVDNIIFISVLAGKLPKAQQG
                     RARTIGLLAAMLMRIALLFSLSWLSRLTNPLFTVVGLDISGRDLILLLGGVFLIAKST
                     WEIHENLEGSVHGAREKAVASFSGLIVQIMLLDIVFSLDSVITAIGMARHIAVMVAAV
                     VIAVGVMLVAARPIAEFVDRHPTVKMLALSFLLLIGFSLVVEGLHQHIPKGYIYFAMG
                     FSVFVEMINLRVRKLREPVQLQRRLQEE"
     gene            1529..1681
                     /locus_tag="PKK95_06975"
     CDS             1529..1681
                     /locus_tag="PKK95_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17991.1"
                     /translation="MDFLLCVAIFLALAFLVVPVAILATAAVLNRRRDSEPSSPDGMS
                     LPTRTV"
     gene            1700..2671
                     /locus_tag="PKK95_06980"
     CDS             1700..2671
                     /locus_tag="PKK95_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC17992.1"
                     /translation="MDEHEKTATGRDIILEIVRAMQGNVEPLLFSTIAPTRYCVYLHP
                     ADYERLEGIFPLVADQARRALDQEVERWNEAARRTRLPRKWLGGREPAPPIEPPADGW
                     DVRFEPDVDGELAPGDIAVASDLTLPARPELEGSKTRRVTTLRRGAEVSRREQVLDVP
                     PAAVRPVSVPPLPAVESPAPPAPASSPRAGLPDPPSAGLPDDRTVYAMLAYEDRQGPH
                     RVAVTTNQVVIGRGGVGYWVDVKLDVSADVSREHLRLRRDEASGQFFLKDLSSLGTSL
                     DGVPVPTSIEVVDGRKRDIGVEVPLPPRARIGLANVIVLDFELAVTP"
     gene            2756..4453
                     /locus_tag="PKK95_06985"
     CDS             2756..4453
                     /locus_tag="PKK95_06985"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOC17993.1"
                     /translation="MPDTAIRHPAIAAAAASDVGLRRENNEDRYTCDPASGLFVVVDG
                     VGGHAAGEHAAETALRMLRTRLSRETGSPGDRLREAITLANNEVHRLAGSDPALEGMA
                     CVLTAALVADGRLTAGHVGDTRLYTFARGAARKLTHDHSPVGEREDRGELDERDAMRH
                     PRRNEIYRDVGSAAHNPNDDEFVELVDVPFEPESAFLICSDGLSDQVSSPEIARIVYE
                     HPDDPATIVGRLVQAANEAGGKDNVTAVFFAGERFAEVARREWPRRAASPEDHDTPRT
                     RARERGRWFLSPPLLAGYGVLAGVALAALSFVLLEPVAGWLLEQSRPASWARTWRVEP
                     EGGGDAATIGEALRLASPGDTISVAPGEYREGVLVDRDLTLVSRNPRQARLAPPAAAE
                     QPWTAVQVRAGVASRISGFAIAASPGAPLAVGIAVGQAAVEIDDVEISGAETAGLLLE
                     NGSRALLRSSYVHDNPGTGVVVAPGALPRLLHNVIAANGRGARSGHAGIEVAPGVLPE
                     LFGNIVAGNGPAEVAGPSAALLQEWQHHNTIGIAAPAKPARPGGARRSPAPPVPTIR"
     gene            4462..6444
                     /locus_tag="PKK95_06990"
     CDS             4462..6444
                     /locus_tag="PKK95_06990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC17994.1"
                     /translation="MLEREFDRYRLIRRLGRGGMADVYLAHDNERGAEVALKIVEMGS
                     DADSREICDAERRGAILQDQFSRVASHVPLVNRWGTFGSWFFIDMEYVEGEDLAERAA
                     RGRLPADEAAWIGAEICSFLERAHAFEAEVEGTRVRGIIHGDLKPRNVRVTPGGQIKV
                     LDFGIAKGLSLSRKLTRNDFGSVTYMSPERLDSGDVDVHSDLWSTGVLLYELVAGSVP
                     FHADSTPRLEARIRAREPVSPLPETCPPALARVILKQLAGDISRRYTSAAELRTDLEA
                     FREGRETRADHEWLGGDPELTRRTQEPDATRRTSPLAGPDAGETARAPVEASPDEEPT
                     QRTAAGAAGQKEAEPDGSDVGAGPASSPRHSTARRVLRIAFVVGLMLVLMNEASVWSD
                     ARQLRVDLATRPSSEAPQLWDRYRSLSSRSLLRIGVAGLRGPMRERLMAQAERVIADY
                     RQDAPAVREAQWRQAASALTDVLHLDPSDRPALARLRYCEGHLQRIDGETRKRRRQPA
                     AQPLHTAAQKFEEAAQLDGRWPDPYLGLARTYIYGLDDLDRAIAALHEAENRGYRPGN
                     RELVQLADGYRSRAERMRRDAQGVRGLDQEKDYLQKALEDYKQAFDLYRQAMGFGEVS
                     AHMRQVQDRIDEVERRLDEIKGRGLGDLLREIFGKS"
     gene            6444..9812
                     /locus_tag="PKK95_06995"
     CDS             6444..9812
                     /locus_tag="PKK95_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOC17995.1"
                     /translation="MPLTRSTAAQRAAASSAARAATRTIELAALLAATLATLAAVGLA
                     AQARLAGVAGVDAAITEGRVVDLRAVDRADPLLPLLEPVVPDSSERRFLAERVASWLA
                     AGDQGGRRRIGSVASLAAARLGEGDIPRTRRLPQLRERFDAARGARPARAPGEAAEPL
                     RVPLLTSAQLAAIRPALVVRRPSDFKWSLAWAALLFVLPFYGVHAWLRLRRSQADRLL
                     LPAVHLLAGIGLALMIGLRDPVRDVLLFGRFAQGVAAGCLLLALAASYDFQRSPVRRL
                     SYIPLLGAVALSALLILFGSGPGSSDAKVNLLGVQPVEAIRLLIVFFLAGYFAERWEF
                     LRELKEPGLSRRAFGLDVPRLDYVLPVLVGVALVLLFFFLQKDLGPALVVACLFLSLY
                     GVARGRATMVVVGLALLVAGFAAGYALDFPRTVVGRVQMWASPWNNAVRGGDQLAHSF
                     WALSTGGITGTGLGLGDPALVPAAHTDLVLAALGEELGLVGVALAFGLLAFIAWRALR
                     VAQRASGDYGFFLALGLALGIVLPFLLIAGGLLGVLPLTGVATPFLSYGRSSMIANLA
                     SVGILLAISSRRSPQVVTGEFARPARVLAGVLAAVLAIAVLRAGYLQAWQADATVASP
                     VITVQGDGARRFSYNPRLLAVAETIPRGAVLDRNGIPLATSRPQDLEKHAATLARLGF
                     RTPSSCAAEGSRCYPFMGVTYHLLGDARSQVNWSASNTSFLERDADARLRGYDDRARL
                     VEVKAPGSTRTDRVLRRDLRELVPLLRHRRRPDHPVVQAFLARPRDVRTSIDIRLQLR
                     AASHLRSTITEANRRRGAAVVLGTDGEVLAAVSYPWPEVAASGSARLQVAAIQPGDES
                     LLDRARYGLYPPGSSFKLVTAAAALRKDPALAGRTFTCVPLPGGRVGQRIPGWGRPIR
                     DDAQDRTAHGAVDLERGLVVSCNAYFAQLGLALGGPALQDAAAQFEIPLGQPESASQI
                     RDTLPFAAYGQGHVLASPLKMARVAATIGNRGRMPDIRWTAGEAEPAEEGREVLAPAH
                     AALLARAMRRVVTEGTGRSLAGIVPQMAGKTGTAEVADAPSHAWFVGFAPYGGTGSRI
                     AYAVIIENGGYGGRTAAPVAAGLVTAARDLGIIK"
     gene            9843..>10607
                     /locus_tag="PKK95_07000"
     CDS             9843..>10607
                     /locus_tag="PKK95_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOC17996.1"
                     /translation="MSILDKMTRLERRLQQLSRRDDVPPEPVEVRRGILDDVEDMVKA
                     AGRSRRVFPYNRLRVQVLAPDGAARAAARAVLGGDAEMLAAIRERLHEGGCSVPGDLQ
                     VKLLLVRKRAAAWPPARAWHVVGERVEGAPHPAPAPSTSAAPETITQLVLVKGTATRR
                     SFPVRGERVNIGRQADVLDRDRRVVRRNQVVFEDSDDPVNQTVSRAHAHLRVTPSGEV
                     RLFDDRSSYGTRVFRGGETIAVPSGASRGVRLRDGDE"
BASE COUNT         1457 a         3849 c         3827 g         1474 t
ORIGIN      
        1 cgatctccgg cgggacggcc accctcgacg ccgaagagcg gcaggtcgac ggcaaggtcg
       61 agctcgaggg ccgcatcgcg gagatccccg tcggcgcgac cgacaccttc tatgtgggcg
      121 agacgatggt gacggtcacg ggcgccacgg tcgtgcgcca cggcgagacc cctgtcgcct
      181 ttgcgacgct gaaggttggc gaccgcgtgc acgtgcgcgg agtcccgggc acggcgccga
      241 actcgatcgc cgcggagctg gtgatcgtgc agaacaccaa cgccaaggtc ccggcgaacc
      301 tgaaaggcaa gatctccgcg ctggtggacg ggacggcctg ccccgcgctc gagttcaagc
      361 tgagcgggtg gaccgtggcg acttcgggct cgacgaccta ctcgaagggc aagagttgcg
      421 tggaccttgc ggccggcacg ggcgtgcacg tgaaggggac cgtggtcgcc gggaaggtcg
      481 cggcggggtc ggtccagttc gacaagaagt aggctcaggg ctcgagagct cagggggctc
      541 acgccccctg ggctcttccc tactccccta ctcctcctgc agccgccgct ggagctgcac
      601 cggctcgcgc agcttgcgca cccgcaggtt gatcatctcc acgaacaccg agaaccccat
      661 cgcgaagtag atgtagccct tcgggatgtg ctggtgcagc ccctcgacca ccagcgagaa
      721 cccgatcagc agcaggaagc tcagggcgag catcttcacg gtggggtgcc ggtccacgaa
      781 ctcggcaatc ggcctggccg ccacgagcat cacccccacc gcgatcacca cggccgccac
      841 catcaccgcg atgtgccgcg ccatcccgat cgcggtgatg accgagtcga gcgagaagac
      901 gatgtcgagc agcatgatct ggacgatcag gcccgagaac gaggccaccg ccttctcacg
      961 cgccccgtgc acgcttccct cgaggttctc gtggatctcc cacgtgctct tcgcgatgag
     1021 gaagacgccg cccagcagca ggatcaggtc gcggcccgag atgtcgagcc cgacgacggt
     1081 gaagagcggg ttggtgagcc gggagagcca ggacagcgag aacagcagcg cgatgcgcat
     1141 cagcatcgcc gccagcaggc caatcgtccg cgcccgcccc tgctgggcct tcggcagctt
     1201 cccggcgagc accgagatga agatgatgtt gtccacgccg agaacgatct cgagcgcggt
     1261 cagcgtgagc agggccaccc agatttccgg gttgaggatc cagtccatga gcgtctcctc
     1321 gagcgggccg gcaaccgctc aagcataaac gagcgcgggc cggctcgtct tgtcccaacc
     1381 ggcgcccccg cccgagcgtc caaccgagtg gacgctgcaa ccctccgggc cttcagccag
     1441 tagtacgagg agcaggccgg caagtccggc accaaggggg cggccagccc catccatcac
     1501 cgggcgccgc cgaccccagg gagagaccgt ggacttcctg ttgtgcgtcg cgatcttcct
     1561 cgcactggcc ttcctcgtcg tgccggttgc catcctggcg accgcggcgg tcctcaaccg
     1621 ccggcgcgac agcgaaccct cctctccaga cgggatgtcg ctcccgacac gaacggtgta
     1681 aggtacagtc cggaccctga tggacgagca cgagaagacg gccaccggcc gcgacatcat
     1741 ccttgaaatc gtgcgggcaa tgcagggcaa cgtcgagccg ctgctgttct cgacgatcgc
     1801 cccgacccgc tactgcgtct acctccaccc ggccgactac gagcggctcg aggggatctt
     1861 cccgctcgtc gccgaccagg cccgccgggc gctcgaccag gaggtggagc gatggaacga
     1921 ggcggcacgg cgaacgcgcc tgccgcggaa gtggctcggc ggccgcgagc cggctccccc
     1981 gatcgagccc ccggccgacg ggtgggacgt ccgcttcgag cccgacgtgg acggcgagtt
     2041 ggccccgggc gacatcgccg tcgcgtccga cctcacgctg ccggcccggc ccgagctcga
     2101 aggatcgaag acgcgccgcg tcacgacgct ccggcgggga gccgaggtca gccggcgcga
     2161 gcaggtgctc gacgtgcctc cagcggccgt tcgccctgtc tccgtcccgc ctctaccggc
     2221 cgtcgaatcg cccgccccgc cggcgccagc ctcttctcct cgagctggtt tgccggaccc
     2281 gccttccgcc gggctgccgg acgatcgaac cgtttacgcg atgcttgcgt atgaagatcg
     2341 tcaggggccg caccgtgtcg ccgtgaccac caaccaggtc gtgatcggcc gcggcggcgt
     2401 cgggtactgg gtggacgtga agctcgacgt ctcggcggac gtctcgcgcg agcacctgcg
     2461 cctccggcgc gacgaggcca gcgggcagtt cttcctgaag gacctcagca gcctcgggac
     2521 cagcctcgac ggggtgccgg tgccgaccag catcgaggtc gtggacggcc gcaagcgcga
     2581 catcggcgtc gaggtgccgc tgccgccgcg ggcccgcatc ggcctcgcca acgtcatcgt
     2641 ccttgacttc gagctcgcgg tgactccgtg accaccctct actggcttcg ctacggcggc
     2701 ctggcgctgc tcgtcgccgc cgcggcgctg ctgctcgtgc tcgccaggcg ctccgatgcc
     2761 tgacaccgcg atccgccacc cggcgattgc cgccgcggcc gccagcgacg tcggcctgag
     2821 gcgcgagaac aacgaggacc gctacacgtg cgacccggca agcggcctct tcgtcgtcgt
     2881 cgacggcgtg ggcgggcacg ccgccggcga acatgccgct gagaccgcgc tgcggatgct
     2941 ccgcacgcgg ctctcgcgcg agacgggaag ccccggggat cggctgcgcg aggcgatcac
     3001 cctcgccaac aacgaggtgc accgcctcgc cggcagcgac ccggctctcg aggggatggc
     3061 ctgcgtcctg acggcggccc tcgtcgccga cgggcgcctg acggccggcc acgtgggcga
     3121 cacgcggctc tacaccttcg cccgcggggc cgcgcggaag ctgacccacg accactctcc
     3181 ggtcggcgaa cgcgaggacc gtggcgagct ggacgagcgc gacgccatgc gccatccccg
     3241 gcgcaacgag atctaccgtg acgtgggctc ggcggcgcac aaccccaacg acgacgagtt
     3301 cgtcgagctg gtcgacgtgc ccttcgagcc cgagagcgcc ttcctgatct gctcggacgg
     3361 cctgagcgac caggtttcct cgcccgagat cgcccgcatc gtctacgagc atccagacga
     3421 cccggcgacg atcgtcgggc ggctcgtcca ggcggcgaac gaggcgggcg ggaaggacaa
     3481 cgtgaccgcg gtgttcttcg cgggcgagcg ctttgcggag gtcgcccggc gcgaatggcc
     3541 gcggcgcgcc gccagcccgg aggaccacga taccccgcgc acgcgagccc gtgagcgcgg
     3601 acggtggttc ctgtcgccgc cgctgctcgc cggttacggc gtgctggcgg gcgtggcgct
     3661 ggccgccctg tcgttcgtcc tgctcgagcc ggtggcgggg tggctgctcg agcagtcgcg
     3721 cccggcctcc tgggcgcgca cctggcgcgt cgagccggag ggtggaggcg atgccgccac
     3781 gatcggcgaa gccctgcggc tggcctcgcc cggcgacacg atctcggtcg cgcctggcga
     3841 ataccgcgaa ggcgtgctgg tggaccgcga cctgacactg gtcagccgca acccgcggca
     3901 ggcccggctg gcgccgccgg cggcggccga gcaaccctgg acggccgtgc aggtgcgggc
     3961 cggagtcgct tcccgcatct cgggattcgc catcgccgcg agccccggtg cgccgctggc
     4021 cgtcgggatc gcggttggcc aggccgccgt ggagatcgac gatgtcgaga tcagcggcgc
     4081 cgagaccgcg ggcctgctcc tcgagaacgg atcgcgcgcc ctgctcaggt cgagttacgt
     4141 gcacgacaac ccggggactg gcgtcgtggt tgcgccgggc gcgctcccgc ggctgttgca
     4201 caacgtgatc gcggccaacg ggcgaggagc acggtcgggc catgccggca tcgaggtggc
     4261 acctggcgtg ttgcccgagc tgttcggcaa catcgtcgcc ggcaacgggc cggcggaggt
     4321 tgccgggccc tcggcggcgc tgctccagga gtggcagcac cacaacacga tcgggattgc
     4381 cgcgccggcc aagccggcga ggccgggcgg agcacgacgg tcgccggcgc ccccggtgcc
     4441 gacgatcagg tgagggcggg cgtgctggag cgtgagttcg acaggtaccg gttgatcagg
     4501 cgcctcgggc gaggcgggat ggccgacgtc tatctcgccc acgacaacga gcgcggcgcc
     4561 gaggtggccc tgaagatcgt cgagatgggc agcgacgccg actcgcgcga gatctgtgat
     4621 gccgagcgcc gcggcgccat cctccaggac cagttcagcc gggtcgcctc gcacgtgccg
     4681 ctcgtcaacc ggtggggcac cttcggcagc tggttcttca tcgacatgga gtacgtcgag
     4741 ggggaggacc tggccgaacg ggccgccagg gggaggctgc ccgccgacga ggcggcctgg
     4801 atcggggcgg agatctgcag cttcctcgag cgcgcgcacg ccttcgaggc cgaggtcgag
     4861 gggaccaggg ttcgcggcat catccacggc gacctcaagc cgcgcaacgt gcgggtgacg
     4921 ccgggcggcc agatcaaggt gctcgacttc ggcatcgcga agggcctctc gctcagccgg
     4981 aagctgacgc gcaacgactt cggcagcgtg acctacatgt cgcccgagcg cctcgactcg
     5041 ggcgacgtgg acgtgcactc cgacctgtgg tccaccggcg tcctgctcta cgagctggta
     5101 gccggcagcg tccccttcca cgccgactcg acgcccaggc tcgaggcgag gatccgcgcc
     5161 cgcgaaccgg tgtcgccgct ccccgaaacc tgtccgcccg cgctggcgcg cgtgattctc
     5221 aagcagcttg ccggcgacat cagccgccgc tacacctcgg ccgccgagct gcgaaccgat
     5281 ctcgaggcct tccgcgaggg tcgcgaaacg cgcgccgatc acgagtggct ggggggggac
     5341 cccgagctca cgcgccgcac gcaggagccg gatgccacgc gccgaacctc gccgctcgcc
     5401 gggccggacg ccggggagac ggcgagggcg ccggtcgagg cttcgcccga cgaggagcca
     5461 acgcagcgga cggcggcagg cgcggcaggc cagaaggagg ccgaaccgga cggctcggat
     5521 gttggcgcgg ggccggcgtc atcgccccgc cattccacgg cgcgccgggt cctgcggatt
     5581 gcgttcgtcg tgggcctcat gctggtgctg atgaacgagg cgagcgtgtg gtcggacgcg
     5641 cggcagctgc gcgtcgacct ggccacgcgc ccgagcagcg aggcgcccca gctctgggac
     5701 cgctaccgct cgctctccag ccgcagcctg ctgcggatcg gggtggccgg gctgcgcgga
     5761 ccgatgcgcg agcgcctgat ggcgcaggcc gagcgggtga tagccgacta ccggcaggac
     5821 gcgcccgccg tgcgcgaggc gcaatggcgc caggccgcgt cggccttgac cgacgtcctg
     5881 catctcgatc cgtccgaccg gccggccctg gctcgcctgc gctactgcga gggacacctg
     5941 cagcgcatcg acggggagac ccggaagcgg cggcgccagc cggcggcgca gccgctccac
     6001 accgccgcgc agaagttcga ggaggccgcg cagctcgacg gtcgatggcc cgacccctac
     6061 ctcgggctgg cgcgcaccta tatttacggc ctcgacgatc tcgaccgcgc gatcgcggcg
     6121 ctgcacgagg ccgagaaccg cggctaccgg cccggtaacc gcgagctggt gcaactggcc
     6181 gacggctacc gcagccgcgc cgagcggatg cggcgcgacg cgcagggtgt gcgcgggctc
     6241 gaccaggaga aggactacct ccagaaggcg ctcgaggact acaagcaggc gttcgatctc
     6301 taccgccagg cgatgggctt tggcgaagtg agcgcgcaca tgcgccaggt gcaggaccgg
     6361 atcgacgagg tcgagcgccg cctcgacgag atcaaggggc gcggactggg cgacctgctc
     6421 cgcgagattt tcggcaagag ctgatgccac tcacacggag caccgccgcc cagcgcgcgg
     6481 ccgcctcgtc ggccgcccgt gccgccaccc gcaccatcga gctggccgcg ctgctggcgg
     6541 ccaccctggc taccttggcc gccgtggggc ttgcggccca ggcgaggctg gcgggcgtgg
     6601 ccggcgtcga cgcggccatc accgagggcc gcgtcgtcga tctgcgcgcc gtcgatcggg
     6661 ccgacccgct gctgccgctg ctcgagccgg tggtccccga ttcgtccgag cgccgcttcc
     6721 tggccgaacg cgtcgcctcg tggctggcgg ccggcgacca gggcggccgt cggcgcattg
     6781 ggtcggtcgc gtcgctggcc gctgcccgcc tcggcgaggg cgacatcccc cggacgcggc
     6841 ggctcccgca gctgcgcgag cggttcgacg ccgcgcgcgg cgcacgcccc gctcgagccc
     6901 cgggcgaagc agccgaaccg ctgcgcgtgc cgctgctgac cagcgcgcag ctggcggcca
     6961 tccgtcccgc gctcgtcgtg cgccgtccat ccgacttcaa gtggagcctc gcgtgggcgg
     7021 cgctcctctt cgtgctcccg ttctacggcg tgcacgcctg gctgcgcctc cggcggtcgc
     7081 aggccgaccg cctcctcctt cccgcggtgc acctgctggc cggcatcggc ctggcgctga
     7141 tgatcgggct gcgcgacccg gtgcgggacg tgctgctctt cgggcggttc gcgcaggggg
     7201 tggcggccgg ctgcctgctg cttgccctgg cggccagcta cgacttccag cgctcgccgg
     7261 tcaggcgcct cagctacatc ccgctgctcg gcgccgtggc gctctcggcg ctgctgatcc
     7321 tgttcggaag cggcccgggc agcagcgacg caaaggtgaa cctcctcggc gtgcagccgg
     7381 tggaggcgat ccgcctgctc atcgttttct tcctcgcggg gtacttcgcc gagcggtggg
     7441 agttcctgcg cgagctgaag gagcccggcc tgtcgcgccg cgccttcggc ctcgacgtgc
     7501 cgcggctcga ctacgtgctg ccggtgctgg tgggggtcgc gctcgtcctg ctgttcttct
     7561 tcctgcagaa ggacctcggc cccgcgctcg tcgtggcgtg cctgttcctg tcgctctacg
     7621 gcgtcgcacg cggtcgcgcg acgatggtgg tggtcggcct ggcgctcctg gtcgccggct
     7681 tcgccgccgg ctacgcgctc gacttcccgc gaacggtggt cgggcgagtg cagatgtggg
     7741 cctcgccgtg gaacaacgcc gtgcgcggcg gcgatcagct ggcgcactcg ttctgggcgc
     7801 tgagcaccgg cgggatcacc ggcacgggcc tcggcctggg cgacccggcc ctggtgccgg
     7861 cggcgcacac cgacctggtc ctcgcggcgc tcggcgagga gctggggttg gtcggcgtgg
     7921 cgctcgcctt cggactgctc gcgttcatcg cgtggcgggc gctccgggtc gcccagcgcg
     7981 cctccggcga ctacggcttc ttcctggccc tgggcctggc cctcggcatc gtgctgccgt
     8041 tcctgctcat cgcgggcggg ctgctcggcg tgctgccgct gacaggggtg gcgacgccct
     8101 tcctcagcta cggccgctcg tcgatgatcg cgaacctggc gtcggtcggg atcctgctgg
     8161 ccatcagcag ccggcgctcc ccgcaagtgg tgacgggcga gttcgctcgc cccgcgcgcg
     8221 tcctggcagg ggttctggcc gccgtgctcg cgatcgcggt gctgcgcgcc gggtacctgc
     8281 aggcctggca ggcggacgcc accgtggcct cgccggtcat caccgtgcag ggagatggcg
     8341 cgcgccgctt ctcctacaac ccgcgcctgc ttgccgtggc ggagaccatc cccaggggcg
     8401 ccgtgctcga tcgcaacggc atcccgctgg caacgagccg gccgcaggat ctcgagaagc
     8461 acgccgcgac gctggcgcgc ctcggcttcc gcaccccctc gtcgtgcgcg gccgagggaa
     8521 gccggtgcta cccgttcatg ggtgtcacct accacctgct cggcgacgcg cgcagccagg
     8581 tgaactggtc ggcgagcaac acgtcgttcc tggagcgcga cgccgacgca aggctgcgcg
     8641 gctacgacga ccgcgcgcgc ctggtcgagg tgaaggcccc gggcagcacc cgtaccgacc
     8701 gcgtgctgcg ccgcgacctg cgcgaactgg tgccgctgct tcgtcaccgg cgccgacccg
     8761 accatccggt cgtgcaggcc ttcctcgcgc gcccgcgtga cgtgcgcact tcgatcgaca
     8821 tccgcctgca actgcgcgcc gcctcgcacc tgcgctcgac aatcaccgag gccaaccggc
     8881 gccgcggcgc ggcggtcgtg ctcggcacgg acggcgaggt gctggcggcc gtgagctatc
     8941 cctggccgga ggtggccgcc tcgggaagtg cccggttgca ggtggcggcc atccagccgg
     9001 gcgacgagag cctgctcgac cgggcgcgct acggcctcta cccacccggc tcgagcttca
     9061 agctggtgac ggccgccgcc gcgctgagga aggacccggc gctcgcgggc cggaccttca
     9121 cgtgcgtgcc gctccccggg ggccgcgtcg gccagaggat cccagggtgg gggcgaccca
     9181 tcagggacga cgcgcaggac cgtaccgcgc acggcgcggt ggatctcgag cgcggcctcg
     9241 tcgtctcgtg caacgcgtac ttcgcgcagc ttggtctcgc gctcggcggc ccggcactcc
     9301 aggatgcggc cgcccagttc gagatcccat tggggcagcc ggagagcgcc tcgcagatcc
     9361 gcgacacgct gccctttgcc gcctacgggc aggggcacgt gctggcctcg cccctgaaga
     9421 tggcccgcgt ggcggcgacg atcggcaacc gtggccgcat gcccgacatc cgctggacgg
     9481 ccggcgaggc cgagcccgcg gaggaaggac gcgaggtgct cgccccggcg cacgccgcgc
     9541 tgctcgcccg cgcgatgcgc cgcgtcgtga ccgagggaac cggccgcagc ctcgccggga
     9601 tcgtcccgca gatggcgggc aaaaccggca ccgccgaggt ggccgacgca ccgtcgcacg
     9661 cgtggttcgt ggggttcgcg ccctacgggg ggacgggcag ccggatcgcc tatgccgtga
     9721 tcatcgagaa cggcggctat ggaggccgca ccgccgcccc ggtggcggcc gggctggtca
     9781 cggccgcccg ggacctgggg atcatcaagt aggatttcgg gattcaggat tcgggacgca
     9841 ggatgagcat tctcgacaag atgacccgcc tcgagaggcg ccttcagcag ctgtcgcggc
     9901 gcgacgacgt gccgcccgag ccggtggagg tgcgccgcgg catcctggac gacgtggagg
     9961 acatggtgaa ggcagcgggc cggtcgcgcc gcgtcttccc gtacaaccgg ctgcgggtcc
    10021 aggttctcgc cccggacggc gcggcacgcg cggccgcccg ggcggtgctc gggggggacg
    10081 ccgagatgct ggccgcgatc cgcgagcggc ttcacgaggg agggtgcagc gttccgggtg
    10141 acctgcaggt gaagctgctg ctcgttcgca agcgtgcggc cgcgtggcct cccgcccgcg
    10201 cctggcacgt cgtgggcgag cgcgttgaag gcgcgccgca cccggctccc gcgccttcta
    10261 cttctgccgc accggaaacc atcacgcagc tggtcctcgt gaagggcacc gccacgcgcc
    10321 gcagcttccc ggtgcgcggc gagcgggtga acatcggccg ccaggccgac gtgctcgacc
    10381 gcgaccgccg cgtcgtgcgg cgtaaccagg tggtgttcga ggattccgac gacccggtca
    10441 accagaccgt ctcgcgggcc cacgcccacc ttcgcgtgac gccgtcgggc gaggtcaggc
    10501 tcttcgacga ccgcagctcg tacggcacgc gcgtgttcag gggcggcgag acgatcgccg
    10561 tgccctcggg ggcgtcgcgc ggcgtccgcc tgcgcgacgg cgacgag
//
LOCUS       DAOPQX010000095        10480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7223_length_10480_cov_5.393765, whole genome shotgun sequence.
ACCESSION   DAOPQX010000095 DAOPQX010000000
VERSION     DAOPQX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7223_length_10480_cov_5.393765"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKK95_07005"
     CDS             complement(<1..414)
                     /locus_tag="PKK95_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOC17997.1"
                     /translation="MKAFDADTLELLADVPAEYAPGKFSRVVGLADVSGQRFVYALFD
                     ANEIRVTDFSDPKNPVTTRYPAGKQPYDGLVTPDGRYFLAGLFGEDGISLLDLWKPEA
                     GARKILAGYGRGEEKLPVFKMPHLRGWSLAAGQAWL"
     gene            complement(330..860)
                     /locus_tag="PKK95_07010"
     CDS             complement(330..860)
                     /locus_tag="PKK95_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17998.1"
                     /translation="MRQPAAVIIVIAVLAFAAPAFAQNAQKATVVDNAPIYLQPDPAS
                     GAVPLRTAKAGTILTVVGEDGDWAQVQFKDPQWGLRTGWVRIAFLKIERPELEPMDLS
                     VGPEDFAPQPGPERAPQVDIGGRRGNAGVPVPAPSRPSSPRTTNPAASRPSTPTPSNC
                     SPTCRPNTLPASSPAS"
     gene            complement(1042..1557)
                     /locus_tag="PKK95_07015"
     CDS             complement(1042..1557)
                     /locus_tag="PKK95_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC17999.1"
                     /translation="MRTRLTALLLAAWLGSAVLSAQTPAGARPADVETPETIVAALYD
                     VISGPAGQARDWDRFRHLFAPGARLMPVAPRKDGSAPAALSPDDYVERTREAFLKNGF
                     FEKEIARKAEAFGNVAHVFSTYETRRAAGDAKPMARGINSIQLMKHAGRWWILTVAWD
                     QEREGNPLPEK"
     gene            complement(2148..2645)
                     /locus_tag="PKK95_07020"
     CDS             complement(2148..2645)
                     /locus_tag="PKK95_07020"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC18000.1"
                     /translation="MEPALAPIAEIYSLNTDLVLNSISGVSEAHAAERPLPGTNSLAF
                     LLAHAIDARYFVARLLGRPGANPLAEIKATGIDDAGELPPLSELRRFWLEASAHLETT
                     LEGLSTADLAATSEQKFPVGDPTVLGAAAFLAQHESYHVGQMALLRKGLGYPAMQYKR
                     PAPNR"
     gene            complement(3022..3582)
                     /locus_tag="PKK95_07025"
     CDS             complement(3022..3582)
                     /locus_tag="PKK95_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18001.1"
                     /translation="MMTTHTRAAAVVVLMLASAGCGGSGGTKAPEQAAASPEGQAGAA
                     AAVTENRWLRGLWANDIRKTLEAVNMPCTGPAMESRQSVWSCESATPLVAYKARFYGN
                     APGKIEYITATITQSDRPKDDLPLRVFVALAGLHFEGADPQKARAWVRANIAAGGSTS
                     FGPAQFKLLGDERRRQLDIKAVGSEW"
     gene            complement(3852..5663)
                     /locus_tag="PKK95_07030"
     CDS             complement(3852..5663)
                     /locus_tag="PKK95_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18002.1"
                     /translation="MGPPARGSAALSFEALSARLARLSDRELLEQVGRMAGEERGATA
                     RLIASLAEMDRRQLYRDAGCSSLFTFCTDVLKLSESEAYLRIEAARAARKFPLVLERL
                     ADGTLTLTAVSKLAKHLTEQNHRALLDSVRQKSKREIEELLARLHPRPDAREMVRRLP
                     EGRAGATSAALAEQPRTRADDPVAFPAGGIDRPVRSGEASGRLDEAAPPGASLLEQAE
                     TSGLRTAAQASGLGTAQQAASTSRAESPALAQSAVRPLPARAAAAAARPRDSIMPLAP
                     QRYRVQLTIGAETNEKLRRVRDLLRHDIPDGDLAQVFDRALTRLLAELEKTRFAATAR
                     PKAPRRPSAPLHSSPKCATPEPDDCLVDGPLKMNPPQRLRVMPPGSTDGRPLGERGAR
                     CSDSEAVLEELEAVTATPVEGALRLPPTGRKKTRRREDFEADSEQAASGKAGSGRAVP
                     GPVVAAAAVTGPAVSGSLAPGSSASGTARQMAAAARRRSRHIPAAVKREVWRRDEGRC
                     TFVGPHGRCRERGMLEYHHREPFAAGGKATASNVTLLCRAHNSLEADRYFGPPMSDVD
                     RYGTRVPPAPQGAHRAAGDVAAAPGSLNPGHPGAGWR"
     gene            5695..6042
                     /locus_tag="PKK95_07035"
     CDS             5695..6042
                     /locus_tag="PKK95_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18003.1"
                     /translation="MLHLYHRHFCHLSNVPAAASAVIAMPDHAKRRPAIGSLSNSNVV
                     ASRAILCPAAREDRGRRREGVKPFLRENGPQRISNFVTVNPVKPEGREPELRPDRVDP
                     LRLKVPSLFSRWS"
     gene            complement(6196..6759)
                     /locus_tag="PKK95_07040"
     CDS             complement(6196..6759)
                     /locus_tag="PKK95_07040"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOC18004.1"
                     /translation="MPRVAPFDRPATYDDLAKLPDHLVAEIVDGELHASARPAPRHAL
                     AGTGIGILVAPAFHHPDPGGWWILYEPELHLGADVVVPDWSGWRRSRMPRLPDEAFFA
                     LRPDWVCEIVSPSTAALDRGRKLAIYAREGIPHCWLIDPAAGTLEVLQLESGRWTILA
                     THVGDVVVRAAPFEATELPLARLWADE"
     gene            6883..7236
                     /locus_tag="PKK95_07045"
     CDS             6883..7236
                     /locus_tag="PKK95_07045"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOC18005.1"
                     /translation="MARELEDLRLPAPVRSTLDAFAESLRARFGPRLHSIRLFGSYAR
                     GEAHEESDVDCLVLIHGALRADDRAVTDLAADLIWQRDGVVISPMVMSPEEFEAWKAS
                     ERRTPLEIERDGVAL"
     gene            7233..7454
                     /locus_tag="PKK95_07050"
     CDS             7233..7454
                     /locus_tag="PKK95_07050"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOC18006.1"
                     /translation="MTPENRRANADAEMARSGESLRAAALLVDAGLLHDAESRLYYAI
                     YHAAVALLLAEGIEPRSHAGTANCWASTS"
     gene            complement(7500..8060)
                     /locus_tag="PKK95_07055"
     CDS             complement(7500..8060)
                     /locus_tag="PKK95_07055"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOC18007.1"
                     /translation="MPRVAPFDRPATYDDLANLPDNLVGEIVEGELHASPRPAPRHLG
                     AASRLGGLLVPRFDRPPDGGWRILDEPELHLGNDVCVPDIAGWRRERLPRLPDEAFFV
                     LAPDWACEILSPSTAALDRGRKLAIYAREAVPHCWLIDPLARTLEVLQLEPGRWTIVA
                     THVGDVVVRAAPFEAIELPLGELWEE"
     gene            8681..9409
                     /locus_tag="PKK95_07060"
     CDS             8681..9409
                     /locus_tag="PKK95_07060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC18008.1"
                     /translation="MRLTLTLAALALLGAATPSGQAPQTNPQVPPGQAAGAKPAAVPA
                     AAAAKPAAVPAAAAAKPAAVPAPAPSDALVPVVIETAMGEIEIVVDTKRAPSTAANFL
                     AYVDAGLYDGGSFHRTVTPANQPNDAVRIEVIQADIPGTKEGELRPPIPLERTSVTGL
                     RHTNGAVSMARSGPDTAQSSFFICIGDQPSLDFGGKRNPDGQGFAAFGLVTRGMDVVR
                     RIQASPAKAQTLSRPIAIVKVRRR"
     gene            complement(9426..9581)
                     /locus_tag="PKK95_07065"
     CDS             complement(9426..9581)
                     /locus_tag="PKK95_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18009.1"
                     /translation="MRGYVNARSSATWRPAGSAYRLLALSKGRRAVYELGPVPVRFEL
                     TAVSGGQ"
     gene            complement(9718..>10480)
                     /locus_tag="PKK95_07070"
     CDS             complement(9718..>10480)
                     /locus_tag="PKK95_07070"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOC18010.1"
                     /translation="GSFTVSSSTGCSWTATSNAAWITLGTTSGSGNGTVSYTVAANTG
                     TSARTGTVTAGGKTFTVTQAGATVTPPPTSPLPTPWQHRDIGLVGRAGDATASGGVFN
                     VSGAGTAFTGTADAFHFAYRNAATEVIASLTSLSDDCPNGRAGVMLRENVGVSARFVA
                     IVALPQGGFEVLHRVTPNSRPSVVARVTQGMLGSLRLVRNKKVITVYRSDDGTSWTQV
                     ATVNVKFTPTLGGLAVTSGSGNLLNAATFAQATAR"
BASE COUNT         1514 a         3753 c         3640 g         1573 t
ORIGIN      
        1 cagccaggcc tgcccggcgg ccagcgacca gccgcgcagg tggggcatct tgaagaccgg
       61 cagcttttct tcgccgcggc cgtagccggc gaggatcttc cgggcgccgg cctcgggttt
      121 ccagaggtcg agcagcgaga tgccgtcctc gccgaacagg ccggccagga agtagcggcc
      181 gtcgggggtg acgaggccgt cgtagggctg cttgccggcg gggtagcggg tggtgacggg
      241 atttttcggg tcggagaaat cggtgacgcg gatttcgttg gcgtcgaaga gggcgtacac
      301 aaagcgctgg ccggacacgt cggccaggcc tacgacgcgg gagaacttgc cgggagcgta
      361 ttcggccggc acgtcggcga gcagttcgag ggtgtcggcg tcgaaggcct tgatgccgcc
      421 gggttcgtag ttctgggcga cgagggcctc gatggtgccg gcaccggaac gcccgcattc
      481 ccccgtcgcc ctcctatatc gacctgcggc gcccgttccg gccctggctg cggcgcaaag
      541 tcctccggcc ccaccgacag atccatcggc tcgagctcgg gccgctcgat cttcaggaag
      601 gcgatcctga cccagcccgt ccgcagtccc cactgggggt ccttgaactg cacctgcgcc
      661 caatccccgt cctcaccgac gacggtcagg atggtgcctg ccttggcggt ccgcagcggc
      721 acggcgcccg acgccggatc cggctgcagg tagatcggcg cgttgtcgac gacggtcgcc
      781 ttctgcgcgt tctgcgcgaa ggcgggtgcg gcgaacgcca ggacggcgat gacgatgatg
      841 acggcggccg gctgcctcat gtgacttcct cccggccggg agcggatccc ggcgcgaacg
      901 agaggaagga tgaacgccgg ccgcgacaga tggggtcact ggggacagga cccgctggcc
      961 gtggccgccg cgtcgtcacg ctttggaggc cgtacgggaa ccgtgaacaa cctcacatcc
     1021 tcggtacggc cccctattgt gctacttctc gggcaggggg ttgccttcac gctcctggtc
     1081 ccacgccacc gtgaggatcc accagcggcc cgcgtgcttc atcagctgga tggagttgat
     1141 gccgcgcgcc atcggcttcg cgtcgcccgc cgcccgccgc gtctcgtagg tgctgaacac
     1201 gtgggcgacg tttccgaagg cctcagcctt tcgagcgatc tccttctcga agaacccgtt
     1261 cttcaggaag gcctcgcgcg tccgctcgac gtagtcgtcg ggcgacaacg cggccggggc
     1321 gctgccgtcc ttgcgcggcg cgaccggcat cagccgggct cccggcgcga agaggtgccg
     1381 gaagcggtcc cagtcgcgcg cctggccggc cggtcccgag atgacgtcgt acagcgccgc
     1441 gacgatcgtc tccggcgttt cgacgtcggc tggtctcgcg cccgccgggg tttgtgctga
     1501 aagcaccgcg ctgcccaacc acgcggcaag caggagcgcc gtcaacctcg ttcgcatgat
     1561 catcctccag gcaacagggg ccgttggcag agcaacaggg acaacggggt tccggaaagc
     1621 gttatcgtaa ggcatacgcc ggagccacga tggccggccg cgacaggctg aagtgcggct
     1681 gaggcggcaa cgcccggcca cgcgcgtcct cggacgtgat tgtcatcggt cgcggagcgc
     1741 gccccgggcc gcgaccagaa cggggcccgc ccggcgcggg cccgcaccga gaacgtgaag
     1801 aacgtcacgg tttcggtacg gccccccgtg gcgtgagaag actcaccatc tccgtacggt
     1861 cccccgtggc ccccctgccg agcggcggtg tgtcggaggg tgtcgatcag cgggtggcgc
     1921 cggtcggggg acccccggcg cgcccggagc gcgtcccccg ttggagtgct tttcgcgcga
     1981 ggacgcgacg ggggtgaccg gcgacaggac ccgctggccg taggcgccgc gacgtcacgc
     2041 acctatacgc cgcacggcat ctgtcgcaag ctgctctgcg gctcgcaggt tgtttctaca
     2101 ctcttccttt ggcatcttgc attcaggccg cgttcttcag cggcctgcta gcggttcggc
     2161 gcgggccgct tgtactgcat ggcggggtag ccgagaccct tccgcagcag cgccatctgc
     2221 ccgacgtgat acgactcgtg ctgcgcgagg aacgcggcgg caccgaggac cgtcgggtct
     2281 ccgaccggaa acttctgttc cgaggttgcg gcgaggtccg cggtcgacaa cccttcgagg
     2341 gtcgtctcga ggtgcgccga cgcctcgagc cagaaccggc gcagctcgct cagcggcggc
     2401 agctccccgg cgtcgtcgat gccggtggcc tttatctcgg cgagcgggtt cgctcccggc
     2461 cgcccgagaa gcctcgccac gaagtagcgg gcgtcgatcg cgtgcgccag gaggaaggcg
     2521 aggctgttgg tgccgggcag cggccgctcc gccgcgtgcg cctccgagac gcccgagatg
     2581 gagttcagca ccaggtcggt gttgagcgag tagatctccg cgatcggggc aagggcaggt
     2641 tccatgttcg agtgacctcc agatcactgt agcttagaac gcggaaggcg gacggagacg
     2701 cggcactctt ggtacgggcc cccgaagcgt gaagaaggca gcgctctcgg tacggtcccc
     2761 ttacgcttcc tgcagagccc gcctcaccac tccgacccga cggccttgat gaacctggcc
     2821 gtgcggtccg ctgcgtgtgg tgtcgaccag cgggtggcgc cggtcggggg acccccggcg
     2881 cgcccggagc gcgtcccccg ttggagtgtt tttcgcgcga ggacgcgacg ggggtgaccg
     2941 gcgacaggac ccgctggccg ccaacgaggc gacgtagcgc actgacgccg cacggcctcc
     3001 aaccccaaat cgcggcccgt ctcaccactc cgacccgaca gccttgatgt cgagctgccg
     3061 gcggcgctcg tcgcccagca gcttgaactg ggccgggccg aacgaggtcg atccccctgc
     3121 cgcgatgttc gcccgcaccc acgcgcgcgc cttctgcggg tcggccccct cgaagtgcag
     3181 cccagcgagg gcgacgaaga cgcgcagcgg caggtcgtcc ttcgggcgat cggactgcgt
     3241 gatggtcgcc gtgatgtact cgatcttgcc gggggcgttc ccgtagaacc gcgccttgta
     3301 agcgacgagc ggcgtcgccg actcgcacga ccacacgctc tgccggctct ccatcgcggg
     3361 tcccgtgcac ggcatgttca ccgcctcgag cgtcttgcgg atgtcgttcg cccacaggcc
     3421 ccgcagccag cggttctccg tcaccgccgc cgccgcgccg gcctgacctt cgggggacgc
     3481 ggccgcctgt tcgggcgcct tcgttccgcc cgatcccccg caccccgccg acgccaacat
     3541 caggaccacg accgccgccg cccgcgtgtg agtggtcatc atcccgctcc ggtgctgcta
     3601 gatcggccct gagacgcagc cgctcgaagg cggtgcccgc ctcgcagcgt gaatataccc
     3661 ctgcgggcgt ctctctgcgg atccctttgg gaccgtccag acccgccggc ggcgcgcgcc
     3721 gcacgtcccc gcttcacctc gccatgtccc gccgcagctg cagcggcgag cactggactt
     3781 cgcgacttcg tgacttcgtg acttcgtgga cttcctgcga actccgtccg gacggagttg
     3841 agcgcttcgc gtcaacgcca gccagcaccc gggtggccgg gattcaggct tcccggggcg
     3901 gcggccacat cgccggctgc ccggtgcgcg ccctgcggcg ccggcgggac tcttgtgccg
     3961 taccgatcga cgtccgacat gggcggcccg aagtagcggt cggcctccag cgagttgtgg
     4021 gctcgacaga gaagggtcac gttcgacgcc gtcgccttcc cgccggccgc gaagggctcg
     4081 cgatggtggt actcgagcat cccgcgctcc cggcatcggc cgtgcgggcc gacgaaggtg
     4141 catcggcctt cgtcgcgccg ccacacctcg cgcttcacgg cagccggaat gtggcgcgag
     4201 cggcgccggg cggcggctgc catctgccgc gccgttcccg acgccgacga ccccggcgcc
     4261 aatgaccctg acaccgccgg tcccgtcacc gccgccgccg ccaccactgg cccggggacc
     4321 gcccgccctg accccgcttt gcctgacgcc gcctgctcgg agtccgcttc gaaatcctcg
     4381 cgccgccgcg tcttcttccg gcccgtcggc ggtaaccgca gagcgccctc cacaggagtc
     4441 gccgtgacgg cctcgagctc ctcgagcacg gcctcggagt ccgagcagcg ggcgccgcgt
     4501 tcgccgagtg gccgaccatc cgtcgaaccg ggcggcatca ctcgcagccg ctgcggcggg
     4561 ttcatcttca ggggaccatc gaccaggcag tcgtccggct caggcgtcgc gcacttcggc
     4621 gacgagtgca acggggccga cgggcgtcgc ggcgccttcg gacgcgcggt tgccgcgaag
     4681 cgcgtcttct cgagctccgc cagcaggcgg gtgagcgcgc ggtcgaagac ctgcgccaga
     4741 tcgccgtcgg ggatgtcgtg ccgaagcagg tcgcgcaccc ggcgcagctt ctcgttcgtc
     4801 tcggcgccga tcgtcagctg cacccggtag cgctgcggcg cgagcggcat gattgaatcc
     4861 cgcggtcggg ctgcagccgc ggccgcccgt gcgggtagcg gccgaacggc cgattgagca
     4921 agagccgggc tttctgccct ggacgtcgac gccgcctgct gcgccgttcc caagccgctg
     4981 gcctgcgccg ctgttcgcag accgctggtc tccgcctgct cgagcagcga cgccccagga
     5041 ggcgcggcct catccagtcg cccgctggcc tctccgcttc gcacggggcg atcgatcccg
     5101 cctgccggaa aggcgaccgg gtcgtcggcc cgggtacggg gctgctccgc gagcgccgcg
     5161 gacgttgctc cggcccttcc ctcgggcaac cgccgcacca tctcgcgcgc atcgggccgc
     5221 gggtgcaggc gcgcgagcag ttcttcgatc tcgcgcttgc tcttctgccg caccgagtcg
     5281 agcagcgcgc gatgattctg ctcggtgagg tgcttcgcca gcttgctcac cgccgtcagc
     5341 gtgagggtgc cgtcggcgag gcgctcgagg acgagcggga acttccgcgc ggcgcgcgcc
     5401 gcctcgattc gcaggtacgc ttcgctctcg ctcagcttca agacatcggt gcagaaggta
     5461 aagagcgacg agcagccggc gtcgcgatag agctggcgcc tgtccatctc ggcgagcgag
     5521 gcaatcagcc gcgccgtcgc gccgcgctcc tcgcccgcca tccggccgac ttgctcgaga
     5581 agctcccggt cggagagccg ggcgaggcgt gccgacagcg cctcgaagga caaggcggcg
     5641 gacccgcgcg caggcgggcc caacggaggg tcagacgatg gtgggagctt gttcatgctc
     5701 cacctatacc atcgtcattt ttgccatctt tcgaacgtgc ctgcggccgc cagcgcggtg
     5761 atcgcaatgc ccgatcacgc gaagcggcgg cccgcgatcg ggagcctctc aaacagcaac
     5821 gtcgtcgctt cgcgagccat cctgtgccca gccgctcgcg aagatcgcgg ccggcgccgg
     5881 gaaggtgtca agccttttct tcgcgaaaac ggccctcagc gcatctcgaa cttcgtgacc
     5941 gtcaatccgg tcaagcccga ggggcgagag cctgaactcc gtccggaccg agttgatccg
     6001 ctgcgactca aggttccttc cctgttttct cgttggtcgt gacgcgcagc tgtggtgccg
     6061 gcgggcacag cggcacgcac gactcggaga tcccgcgacc ccgctgtgca tcgaccccgc
     6121 acggcctgca gccccgcttg atcgggggga tgtcaacgcg accggcaggt tcgcccaaga
     6181 ccgcctgctg ttcgactact cgtcggccca cagccgggcg agcggcagct cggtcgcctc
     6241 gaagggagcg gcgcgcacca ccacgtcgcc gacgtgcgtg gccaggatgg tccaccgccc
     6301 ggattcgagc tgcaggacct cgagggttcc ggccgccggg tcgatcagcc agcagtgggg
     6361 aatgccctcg cgggcgtaga tggccagctt gcggccgcgg tcgagggccg ccgtcgaggg
     6421 cgacacgatc tcgcacaccc agtcggggcg cagcgcgaag aaggcttcgt ccgggaggcg
     6481 cggcatccgc gatcgccgcc agcccgacca gtcgggaacg acgacgtcgg cgccgaggtg
     6541 gagttcgggc tcgtagagga tccaccagcc cccgggatcg ggatggtgaa aggctggggc
     6601 gaccaggatg ccaatcccgg ttccagccag tgcgtgccgc ggcgcgggcc tggctgacgc
     6661 gtgcagctcg ccatcgacga tctccgcgac gagatgatcg ggcagcttcg cgaggtcgtc
     6721 gtaggtcgcg ggccgatcga acggcgcaac cctcggcacg tccggcattg tacatcgtcg
     6781 cttcgtgaag tcgcggcggc ggccgcctgg cccgctaatg ctgcctgcga tcgggccgcc
     6841 ccccgcagcc tcggggcggc gaagggggct acaatttccc cgatggctcg cgaactggag
     6901 gacctccggc ttccggcgcc cgttcgttcg acgcttgacg cgtttgccga gagcctccgc
     6961 gcccggttcg ggccgcgact gcactcgatc cgcctgttcg gctcctacgc ccggggggag
     7021 gcgcacgagg aatccgacgt ggattgcctg gtcctcatcc atggcgccct gcgcgccgac
     7081 gaccgcgccg tcaccgacct ggccgccgat ctgatctggc agcgcgacgg cgtcgtcatc
     7141 tcgccgatgg tcatgtcacc cgaagagttc gaggcgtgga aggcgagcga gcgccgcacg
     7201 ccgctcgaga tcgaaaggga cggggtggcg ctatgactcc ggagaaccgc agggccaacg
     7261 ccgacgccga gatggcgaga tccggggagt cgctccgcgc ggccgccctg ctggtggacg
     7321 cgggcctgct gcacgacgca gagagccggc tgtactacgc catctaccat gcggccgtcg
     7381 cgctgctcct cgcggagggg atcgaaccgc ggtcgcacgc cgggaccgcg aactgctggg
     7441 cctccacttc gtgaagtcgg ggcggatggc gccggagacc gccttcgccg gccgcacgct
     7501 cattcttccc acagctcgcc gagcggcagc tcgatcgcct cgaagggcgc ggcgcgcacc
     7561 accacatcgc ccacgtgcgt ggccacgatg gtccaccgcc ccggttcgag ctgcaagacc
     7621 tcgagggttc gggccaacgg gtcgatcagc cagcagtgcg gtaccgcctc gcgggcgtag
     7681 atggccagct tgcgcccgcg gtcgagggcc gccgtcgaag gcgacaggat ttcacaggcc
     7741 cagtcgggcg ccagcacgaa gaacgcttcg tccgggaggc gcggcaaccg ctcgcgccgc
     7801 cagccggcga tatccgggac gcagacgtcg ttccccaggt gcagttccgg ctcgtcgagg
     7861 atgcgccagc ccccatccgg ggggcgatcg aagcgaggca ccagcaaacc ccccaggcgg
     7921 cttgcggccc ccaggtggcg cggcgccggc cgtggcgacg cgtgcagctc accctcgacg
     7981 atctcgccga ccaggttgtc gggcaggttc gccaggtcgt cgtaggtcgc gggccgatcg
     8041 aacggcgcaa ccctcggcac gtccggcatt gtacatcgtc acttctgcac ggctcttgcc
     8101 gccggcgccg gccggaattg gcggggattt cctcagccag cgcccagaga tcgcagttcc
     8161 tgcaccccgt cgcgccgcct gacgttgcct gttcaggcgg ctttcgattc gaaggcggcc
     8221 acggcttggg gcgtgaggtg gaggggctcc tgggggccgt accaaaggcg tgaagaatgt
     8281 cacggtgcgg agggccgtac cgaaaccgtg aagaacacag cactttcggt acggcccccg
     8341 tagagtgaag aacacgacat ccttccgtac agcctccgtg acgattttgg ccgtgcggtc
     8401 cgccggtgta tcgaccagcg ggtggcgccg gtcgggggac ccccggcgcg cccggagcgc
     8461 gtcccccgtt ggagtgcttt tcgcgcgagg acgcgacggg ggtgaccggc gacaggaccc
     8521 gctggccata ggcaccgcga cggaacgcac caacacgccg aacggcctcg agtcctcccg
     8581 gttgaaatcc gaggccgttg ctctctccgc atcggccttg cgcagcagtg cggcaagaga
     8641 cgacgatggc cgtgcgcaaa cggagccggg aaggacgaac atgagactga cgctcacgct
     8701 ggcggcactc gcgctgctcg gcgccgcgac gccgtcgggg caggccccac aaacgaaccc
     8761 acaggtcccg ccgggccagg ccgcgggtgc aaagcccgcg gctgtgcccg cggccgcggc
     8821 tgcgaaaccc gcggctgtcc ccgcggccgc ggctgcgaaa cccgcggctg tccccgcgcc
     8881 tgcccccagc gacgccctcg tccccgtcgt gatcgagacg gcgatgggcg agatcgagat
     8941 cgtggtggac acgaagcgcg cgccgtcaac cgcggcgaat ttcctcgcct acgtggacgc
     9001 ggggctctac gacggcggca gcttccaccg caccgtgacg cccgccaacc agccgaacga
     9061 cgcggtcagg atcgaggtga tccaggccga catccccggg acgaaggagg gcgagctgag
     9121 gccgccgatc ccactcgagc ggaccagcgt gaccggcctt cgtcacacga acggtgccgt
     9181 ctcgatggcg cgctcggggc ccgacaccgc ccagtcgagc ttcttcatct gcatcggcga
     9241 ccagccctcg ctcgacttcg gcggcaagcg caatcccgac ggccagggct tcgccgcatt
     9301 tggcctcgtg acgcgcggca tggacgtcgt ccggcgcatc caggcgtcgc cggcgaaggc
     9361 ccagaccctc tcgcggccga ttgccatcgt gaaggtgcgg cgtcggtagg cgccggttgc
     9421 gcagcctact gcccgcccga cacggcggtg agttcgaatc ggacgggaac cggccccagc
     9481 tcgtacaccg cccggcgccc cttcgagagg gcgagcaggc gatacgcgct gccggccggg
     9541 cgccaggtgg cgctcgacct cgcgttcacg taaccacgca tcgactcggc agcgcgcgcg
     9601 acccgcccgg tcgtgaaacg tgcgtccggc gccaggtcac cgttgaggat tcgccgggcg
     9661 gcccaaatcc tgaatcccga tcccgaatcc tgaaccccgg ctcggtgagc tcggggtcta
     9721 gcgggcggtg gcctgcgcga acgtggccgc gttgaggagg ttgcccgacc cgctggtgac
     9781 cgccaggccg cccagcgtcg gcgtgaactt gacgttcacc gtggccacct gggtccacga
     9841 cgtcccgtcg tccgatcgat acaccgtgat caccttcttg ttgcgaacca gcctgagaga
     9901 ccccagcatc ccctgggtca cccgcgccac cacgctcggg cggctgttcg gcgtcacccg
     9961 gtgcaggacc tcgaacccgc cctgcggcag cgcgacgatc gcgacgaagc gggccgagac
    10021 ccccacgttc tcgcgcagca tgacgcccgc gcgcccgttg gggcagtcgt ccgacaggct
    10081 ggtcaggctc gcgatcacct cggtggccgc gttccgatag gcgaagtgga aggcgtctgc
    10141 ggtgcccgtg aacgccgtcc cggcgccgct gacgttgaag acgccgccgc tggccgtggc
    10201 gtcgcccgcg cggcccacca ggccgatgtc gcggtgctgc cagggggtcg ggagcggcga
    10261 agtggggggt ggcgtgacgg tcgcacccgc ctgcgtgacc gtgaaggtct tcccgccggc
    10321 cgtcacggtc ccggtccgcg cactggtgcc ggtgttcgcc gcgacggtgt agctgaccgt
    10381 cccgttgccc gagcccgacg tggtacccag ggtgatccac gcggcgttcg aggtcgccgt
    10441 ccagctgcag cccgtggaac tgctcaccgt gaacgagccg
//
LOCUS       DAOPQX010000096        10431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7274_length_10431_cov_4.867290, whole genome shotgun sequence.
ACCESSION   DAOPQX010000096 DAOPQX010000000
VERSION     DAOPQX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7274_length_10431_cov_4.867290"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PKK95_07075"
     CDS             complement(<1..1088)
                     /locus_tag="PKK95_07075"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC18011.1"
                     /translation="MADAAYLETPLRRFKGVGPRREQALRKIGVEKVEDLLLRLPLRY
                     EDRTSFTPVAAVPEGERVTVAGELINCRLRWTGRRGFKVFEAVVRDESGGLLAVWPNQ
                     PYRQNTIRAHARAILHGPVVRYRGVLQLSNPDIEILDEDGGDPLHTGRIVPVYEKAGP
                     VTARMQRRLVHEVLQGLPPDLDDLLPGALREAAGLPGRAEALRDTHFPPAGTPPQELE
                     RFRTPAARRLIFEEFFLFQAGLLERRAEAASRPKPLVPRVDDRVRASVRAALPFRLTA
                     GQRQALAEIVEDMRRPAPMNRLLQGDVGSGKTVVAALAALVALENGFQVALMVPTEIL
                     AEQHLLVLSRLFAQTGFRPALLTGATPAA"
     gene            complement(1093..1716)
                     /locus_tag="PKK95_07080"
     CDS             complement(1093..1716)
                     /locus_tag="PKK95_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18012.1"
                     /translation="MTLPRRLLLVPALVLALGPLGVTTASQGSTGAASRAESRRLVEK
                     LTRIIERGSRPKAGPLRTTITEGELNAYFAHDGTADLPVGVTQPAVVIHGDRRVTGSA
                     VVDLDAVRAHHKPTGMLDPMNYLTGKLPVAAAGVLDARDGKARLQLESASIAGIPVPR
                     AVLQEVVAYYTRSAEFPKGIDIADPFPLPAGIQAIDLGRRGEAVVVQ"
     gene            1838..2443
                     /gene="lexA"
                     /locus_tag="PKK95_07085"
     CDS             1838..2443
                     /gene="lexA"
                     /locus_tag="PKK95_07085"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOC18013.1"
                     /translation="MLPLTKRQREILDYLNEFIQQHGYAPSLEEVGRRFGLSSLATVH
                     KHLTNLQEKGFIKRTWNRSRSVELLQSRAAGRAVDLPLLGYVAAGAPIEAIATSETIA
                     VPEALVGKRDTYVLRVRGESMIDEQIRDGDYVIVEDRKTAENGEMVIALLGNSDVTLK
                     KFYRENGHIRLQPANPSMEALVVPADQVRVQGVVVGVMRKY"
     gene            2457..2771
                     /locus_tag="PKK95_07090"
     CDS             2457..2771
                     /locus_tag="PKK95_07090"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOC18014.1"
                     /translation="MPEPKQINFTIVPSDPGAAPRIYSNFCAIAHTPFDLTLTFCEVL
                     PLSERDLREAESEHTVHAPVRANIVVPLAMVPNLVAALQEHLRVFSEQNPGAAWGKGP
                     VH"
     gene            2779..3411
                     /gene="nth"
                     /locus_tag="PKK95_07095"
     CDS             2779..3411
                     /gene="nth"
                     /locus_tag="PKK95_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOC18015.1"
                     /translation="MKTQAEARRVFRALAAQHPNADTELHYRNPFELLVATILSAQTT
                     DARVNEVTPALFQRYPDAASLASADEEELQAAIRTTGFFRQKARALIGMSRGLVLRHG
                     GRVPPSLEALVTLPGVGRKTANVVLGHALGVPGLPVDRHVLRVANRVGLASGDDPNLV
                     EQQLCALLPKKQWTCASDTLILHGRRICKPRPLCDRCSVRRDCDYYQRRP"
     gene            3408..3851
                     /locus_tag="PKK95_07100"
     CDS             3408..3851
                     /locus_tag="PKK95_07100"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOC18016.1"
                     /translation="MTRAAFRRLVSEAIDTIPPDFQQHVKNMAIVVEDEPPDELLDEM
                     GIEPDDTLFGLYQGTPLTERGWGHGNVLPDRITIFQGPIEDWCEGDREAIVTEIGETL
                     IHELGHYFGLSEDEIQEIEDRYWHKRGGRDEDEEGEEADEDEGPR"
     gene            3848..4639
                     /gene="rsmA"
                     /locus_tag="PKK95_07105"
     CDS             3848..4639
                     /gene="rsmA"
                     /locus_tag="PKK95_07105"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOC18017.1"
                     /translation="MKARKRLGQHFLEPAWVARLVEEIGPGLDETFLEIGPGRGALTL
                     ALAPRVARLVAVEVDRDLARALRRRVPGHVTIVEGDFLETDLGALLEGNVPARVAGNL
                     PYNLSTPILFRLLALADQGRRLRDATLMLQREVADRLGATPGGKIWGVLSACQQLRAD
                     VEKRFVLPPGAFRPAPKVHSAVVTLRYRPPAPPVTDAALFDALVRAIFTQRRKVLDNA
                     LEPFAAARGTTSRAALAEAGLDGRRRPETLSLAELAALAEVFARD"
     gene            4867..6564
                     /locus_tag="PKK95_07110"
     CDS             4867..6564
                     /locus_tag="PKK95_07110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOC18018.1"
                     /translation="MTGPATLAGSPEPGGPHLEVVPLGGLGEFGMNMTAVSWGETLVL
                     VDAGVMFPDAELLGVDLVIPDLAYLEERRVKASALVLTHGHEDHIGAVPHVLPLVDGP
                     VYGTPLTLAMLEPKLEEHGVDARSRLVPVSPRGRVEVGPLAIEFFRVTHSIPDAIALA
                     IETPVGTIVHTGDFKIDQTPLDGEKFDFHRFAELGAGGVLALLGDSTNIERPGVTGSE
                     YDVVAAFEELFASTRGKLVVTSFATSIYRMQILVNLAKDFGRKVAFVGRGMIEQSQVA
                     QRLGKLHVPPGLLIRETEVRAYPARQVLCLTTGSQGEPMSALSRIAVDDHRHVSIGPD
                     DRVVFSARAIPGNEKPIDRVINHLARRGADVVTDGEKRVHVSGHGSEEELKTMLSLVR
                     PRCFIPIHGEYRQRSRYARLAQRLLGGGTQVLLAENGDLIRLDASGARVTGRVATGRV
                     LIDGPGTGEVADEVLRDRRHLAEDGLIVPVLAINKQTGQVEGVPDVITRGLVLEPDAE
                     GLLREAGFLIADVLDQIGLEERTDRGLVKERIRVELRRFFRKRSGRRPLVLPVIMEI"
     gene            6599..8923
                     /locus_tag="PKK95_07115"
     CDS             6599..8923
                     /locus_tag="PKK95_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOC18019.1"
                     /translation="MSEFLGVALFATALIWLIALVTYDPGDPAWFFHAGADRQPANFI
                     GRVGAFVAELSVQVLGFASYLVPVLLGVVGWTLFWCRRVEAVYTKLVGTALLAGCLAS
                     LLHLALESVTLSGRRLAAGGYIGAQISSELTAYFSKTGSFIILVTLLFLSVILATQFS
                     FGRMFSAIGDAIFGSARRAVARLRERREARRRERARREVVRKRMAESPEAVKEIVEKA
                     QPEKVAASAPSKASAADTSRPAAVEAPPRPRPTPAISRPQAAPAARKPAAPAAPVPEA
                     AAAYEGRTPIERKREGFTPPPLALLDAPRSERKIDERELMELARQLEEKCHEFAVAGS
                     VVQIHPGPVVTTFEFKPDAGVKYAKVTGLADDLSLALKAESVLIERIPGKSTVGIQIP
                     NTSREQISLREMLESDLFRRSGSKLTLALGKTIHGEPFVADLATMPHLLIAGSTGTGK
                     SVAINSMLTSILYRATPEDVRLIMIDPKRLELGMYEEIPHLLTPVVVDPKAASNALAW
                     AVREMEERYKTLAAEGVRNIEQYNRNIRHQMEEKREADEEGVELRTLPYVVVVIDELA
                     DLMMVASNEVEESICRLAQMARAVGIHLILATQRPSVDVITGLIKANLPARISFRVSS
                     KVDSRTILDGNGAEQLLGRGDMLYLPPASSRLIRLHGPYISEQESARLAGYLRKQGKP
                     VYDTTITADEKTADGVEFEKDDLYDEAARIVVGSGQASISYLQRRLRIGFSRAARLVD
                     MMESEGLVSPASGSKPREVLVDKGYFDEVDAQLR"
     gene            8923..>10431
                     /locus_tag="PKK95_07120"
     CDS             8923..>10431
                     /locus_tag="PKK95_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOC18020.1"
                     /translation="MILTSRPARLALLAAVALALAVPAGAQRPSGRAAAPAGRERPAQ
                     IRDVKRTRLTEAVRITIELEREVPYRSERLDGPPRLVFDFLGTRPAATLVQGSLSYPD
                     DVVRHIRVGRHPNDVTRVVLDLTGVQRYLVFALYDPYRLVIDCVRAVQPAVTAAARPP
                     ASPPAGSKPAVREPGPAAPEPRPALPAVASAPLPPERVTEHELAAADVEPSTGPVLMF
                     SRRLRVAFVPTAEAPVPAPIELEAEEAPPAAAAPPSAPPPSAPPPPAPAKLSAPATAS
                     SGEKPSTPAPLKGGFSLARQLGLGATRIVIDPGHGGRDPGAQARGISEAAITLDIALR
                     LEKLLKGAGIDVVLTRRTDRYVALEERTEIANRAAGDLFLSIHANASRNTRAQGVESY
                     VLNFASNPEAEAVAARENASSERTMSSLNAILQAIALNNKLDESRGFAASVQNALVRR
                     LRATRRQLKDLGVKQAPFVVLIGAEMPSVLVEVSFISHQQEGRLLRTATYRQR"
BASE COUNT         1534 a         3761 c         3674 g         1462 t
ORIGIN      
        1 gccgccggcg tcgcgccggt gagcagcgcc gggcggaacc cggtttgggc gaagagccgc
       61 gagagcacca gcaggtgctg ctcggccagg atctcggtgg gcaccatcag cgccacctga
      121 aatccgttct cgagcgcgac cagcgccgcc agcgccgcca cgaccgtctt gcccgacccc
      181 acgtctccct gcagcaggcg gttcatcggg gccggccgcc gcatgtcttc gacgatctcg
      241 gccagggcct ggcgctgccc ggcggtcagc cggaacggca gcgccgcccg aacgctggcc
      301 cgcacccggt cgtccaccct gggcaccagc ggcttgggcc gagaagccgc ctcggcgcgc
      361 cgctccagca ggcccgcctg gaacaggaag aactcctcga agatcagccg gcgggccgcc
      421 ggcgtccgga agcgctccaa ctcctgcggg ggcgtccccg ccggagggaa gtgcgtgtcg
      481 cgcagggcct cggcacgccc cggcagccct gccgcctcgc gcagcgcgcc gggaagcagg
      541 tcgtccagat ccggcggcag cccctgcagc acctcgtgca ccagccgcct ctgcatccgg
      601 gccgtcaccg ggccggcctt ctcgtacacc ggcacgatgc gcccggtgtg cagcgggtct
      661 cccccgtcct cgtcgaggat ctcgatgtcc gggttgctca gctgcagaac gccccggtaa
      721 cggacgaccg gtccgtgcag gatggcgcgc gcgtgggcgc ggatcgtgtt ctgccggtag
      781 ggttggttcg gccagacggc gagcaggcca cccgactcgt cgcgaacgac cgcctcgaag
      841 accttgaacc cgcgccgccc ggtccaccgc agccggcagt tgattagctc gcccgcgacg
      901 gtgacccgct ccccttccgg cacggccgcc accggggtga acgaggtgcg gtcctcgtag
      961 cggagcggga gacggagcaa gaggtcctcg accttctcca ccccgatctt gcgcagcgcc
     1021 tgctcgcgcc ggggaccgac tcccttgaac cgccggagcg gggtctccag gtatgcagcg
     1081 tcggccacga gcctactgta cgacgacggc ctcgccgcgt cgaccgaggt cgatggcctg
     1141 gatgccggcg gggagcggga acgggtcggc gatgtcgatg cccttgggga actcggccga
     1201 gcgcgtgtag taggccacca cctcctgcag cacggcgcgc ggcacaggga tccccgcgat
     1261 cgatgccgac tcgagctgga ggcgggcctt cccgtcgcgg gcatccagca cgccggccgc
     1321 cgccaccggc agcttgccgg tgaggtagtt catcgggtcg agcatgcccg tgggcttgtg
     1381 atgggcccgg acggcgtcga ggtccacgac cgcgctgccc gtgacacggc gatcgccgtg
     1441 aatgacgacg gcgggctggg tcaccccgac cggcaggtcg gccgtcccgt cgtgcgcgaa
     1501 gtacgcgttg agctcgccct cggtgatggt ggtccgcagc gggccggcct tgggccggct
     1561 cccccgctcg atgatgcgcg tcagtttctc gaccaggcgg cgcgactcgg cgcggctggc
     1621 tgctccagtg ctcccctgcg aggcggtcgt cacccccaag ggtcccagcg cgagcaccag
     1681 ggcgggcacg agcagcaaac gtcgagggag cgtcacgggc gttctggatc ctgccagaag
     1741 tgtagggcac aacgcgccgg ccggccagcg cttgactttc gcctttcctt tgcatagagt
     1801 gctgggcgga accacccgcc gccccgagga ggaccgcgtg ttgccactca ccaagcgcca
     1861 gcgcgagatc ctggactacc tcaacgagtt catccagcag cacggctacg cccccagcct
     1921 cgaggaggtc ggccgccgct tcggcctctc ctcgctcgcc accgtgcaca agcacctgac
     1981 gaacctgcag gagaaggggt tcatcaagcg gacctggaac cgcagccgct cggtcgagct
     2041 gctgcagtcg cgcgcggcgg ggcgcgccgt cgacctgccg ctgctcggct acgtggcggc
     2101 gggggcgccg atcgaggcga tcgcgacctc cgagacgatc gccgtgcccg aggcgctggt
     2161 cggcaagcgc gacacctacg tgctgcgggt ccgcggcgag tcgatgatcg acgagcaaat
     2221 ccgcgacggc gactacgtca tcgtcgagga ccggaagacg gccgagaacg gcgagatggt
     2281 catcgcgctg ctcggcaact cggacgtgac gctcaagaag ttctaccgcg agaacggaca
     2341 catccgcctg cagccggcga acccgtccat ggaggcgctg gtcgtgccgg ccgaccaggt
     2401 gcgcgtgcag ggggtcgtcg ttggggtgat gcggaaatac tgatatcgtt acccccatgc
     2461 ccgagccgaa gcagatcaac ttcacgatcg ttccgagcga tccgggcgcg gcaccgcgca
     2521 tctactcgaa cttctgcgcg atcgcccaca cccccttcga tctcacgctc accttctgcg
     2581 aggtgctgcc gttgtccgag cgcgacctcc gcgaggcgga gagcgagcac acggtgcacg
     2641 cccccgtccg cgccaacatc gtcgtgccgc tggcgatggt gccgaacctg gtggcggcgc
     2701 tgcaggagca cctgcgcgtg ttctcggagc agaacccggg agcggcgtgg ggcaaggggc
     2761 cggtgcactg aaggcgggat gaagacgcag gccgaagcca ggcgcgtgtt ccgggcgctc
     2821 gcggcgcagc acccgaacgc cgacaccgag ctgcactacc ggaacccgtt cgagctgctc
     2881 gtcgccacga tcctgtcggc gcagaccacc gacgcccggg tgaacgaggt gacgcccgcg
     2941 ctcttccagc gctaccccga cgcggcctcg ctggcatcag cagacgaaga agagctgcag
     3001 gccgcgatcc ggacaaccgg cttcttccgc cagaaggcgc gggcgctcat cgggatgtcg
     3061 cgcgggctgg tcctccggca cggggggcgg gtgccgccca gcctcgaggc cctggtcacg
     3121 ctgcccggcg tggggcgcaa gacggcgaac gtcgtgctcg gccacgcgct cggggtgccg
     3181 ggcctcccgg tcgaccggca cgtgcttcgg gtcgcgaacc gggtcggcct cgcgagcggc
     3241 gacgacccga acctggtcga gcagcagctg tgcgcgctgc tgccgaagaa gcagtggacc
     3301 tgcgcgtccg atacgctcat ccttcacggc cgccgcatct gcaagccccg gcccctgtgc
     3361 gatcgctgtt ccgtgcggcg ggactgcgac tactaccagc gccgcccatg acccgagccg
     3421 ccttccgcag gctggtgtcc gaggcgatcg acacgatccc ccccgacttc cagcagcacg
     3481 tgaagaacat ggccatcgtc gtcgaggacg agccgccgga cgagctgctc gacgagatgg
     3541 ggatcgagcc cgacgacacg ctgttcggcc tctaccaggg caccccgctg accgagcgcg
     3601 gctggggcca cggcaacgtg ctgcccgacc ggatcacgat cttccaggga ccgatcgagg
     3661 actggtgcga gggcgaccgc gaggcgatcg tcaccgagat cggcgagacg ctgatccacg
     3721 agctggggca ctacttcggg ttgagcgagg acgagatcca ggagattgaa gaccgctact
     3781 ggcacaagcg cggcggccgc gacgaggacg aggaaggcga agaggcggac gaagacgagg
     3841 ggccgcggtg aaggcgcgca agcgcctcgg ccagcacttc ctcgagccgg cgtgggtggc
     3901 gaggctggtg gaggagatcg gcccgggtct cgacgagacc ttcctcgaga tcgggcctgg
     3961 ccgcggggcg ctgaccctgg cgctcgcgcc gagagtcgcc cggctggtcg ccgtcgaggt
     4021 ggaccgcgat ctggcgcgcg cgctccggcg gcgggtcccc ggccacgtca ccatcgtcga
     4081 gggcgacttt ctcgagaccg acctcggcgc gctgctcgag gggaacgttc ccgcgcgggt
     4141 ggctggcaac cttccctaca acctctcgac gccgatcctc ttccgcctgc tggcgcttgc
     4201 cgaccagggg cggcggctgc gcgacgcaac gctgatgctg cagcgcgagg tggccgaccg
     4261 gctcggcgca acgccgggcg gcaagatctg gggggtgctc tcggcgtgcc agcagctccg
     4321 cgccgatgtc gagaagcggt tcgtcctgcc tcccggcgcc ttccgccccg cccccaaggt
     4381 gcactcggcc gttgtcacgc tccgctaccg acccccggca ccgcccgtga ccgacgcggc
     4441 gctgttcgac gcgctcgtcc gggccatctt cacgcagcgg cgcaaggttc tcgacaacgc
     4501 cctcgaaccc ttcgcggctg cacgcgggac gacctctcgc gctgccctcg ccgaggccgg
     4561 cctcgatggg cgccggcggc ccgaaacgct gtcgctcgcc gagctggccg cgctcgcgga
     4621 ggtattcgcg cgagactgac gcctgcccga ctgtgcctgc ttaagccaaa gctgtgctat
     4681 agtttgcaca gccttcccgt cctccgcggc cttcccagcg accgcggatg ccgggttgcc
     4741 cggcgccagc cgggcggcca ggccgaacga gcagcggcgc cccggcgttt cggggccgca
     4801 ttgtccgcgc gcaccgcgac gcgctgggat cacccgattg ctccaaccgc cacccggagg
     4861 gtgcccgtga caggtcccgc gacgctggct ggctcccccg aacccggcgg cccgcacctc
     4921 gaggtcgtgc cgctcggggg gctcggcgag ttcgggatga acatgaccgc cgtgtcctgg
     4981 ggcgagacgc tggttctcgt ggatgccggc gtgatgttcc cggacgccga gctgctgggg
     5041 gtggacctgg tgatccccga cctcgcctac ctcgaggagc ggcgggtgaa ggcgtccgcg
     5101 ctggtgttga cgcacgggca cgaggaccac atcggcgcgg tgccgcacgt gctgccgctg
     5161 gtggacggcc cggtctacgg gaccccgctg acgctcgcga tgctcgagcc gaagctcgag
     5221 gagcacggcg tggatgcgcg ctcgcggctc gtccccgtgt cgccgcgggg ccgcgtggag
     5281 gtcgggccgc tggcgatcga gttttttcgg gtgacgcaca gcatccccga cgcgatcgcg
     5341 ctcgccatcg agacgccggt cggcacgatc gtccacacgg gcgacttcaa gatcgaccag
     5401 acgccgctcg acggcgagaa attcgacttc caccgcttcg ccgagctggg ggcgggcggg
     5461 gtgctggcgc tgctcggcga cagcaccaac atcgagcggc ccggcgtcac cggatccgag
     5521 tacgacgtgg tggcggcgtt cgaggagctc ttcgccagca cccgcggaaa gctggtggtg
     5581 acctccttcg cgaccagcat ctaccggatg cagatcctgg tgaacctggc gaaggacttc
     5641 ggccgcaagg tggcgttcgt ggggcggggg atgatcgagc agtcgcaggt tgcccagcgg
     5701 ctgggcaagc tgcacgtccc gccggggctg ttgatccggg agaccgaggt gcgcgcctac
     5761 ccggcccggc aggtgctctg cctgaccacg ggaagccagg gcgagccgat gtcggcgctg
     5821 tcgcggatcg cggtggacga tcaccggcac gtctcgatcg ggccggacga ccgggtggtc
     5881 ttctcggccc gcgcgattcc cggcaacgag aagccgatcg accgggtgat caaccatctc
     5941 gcgcggcgcg gcgccgacgt ggtgaccgac ggcgagaagc gggtgcacgt gtcggggcac
     6001 ggcagcgagg aggaactgaa gacgatgctc tcgctggtcc ggccccggtg cttcatcccg
     6061 atccacggcg agtaccgcca gcgctcgcgc tacgcgcggc ttgcgcagcg gctgctcggc
     6121 ggcggcaccc aggtgctgct tgcggagaac ggcgatctca tccggctcga cgcgtccggc
     6181 gcccgcgtca cggggcgggt ggcgaccggc cgcgtgctca tcgacggccc cggcacgggc
     6241 gaggtggccg acgaagtcct gcgggaccgg cgccacctgg ccgaggacgg gctgatcgtc
     6301 ccggtcctgg cgatcaacaa gcagacgggc caggtcgaag gcgtcccgga cgtcatcacg
     6361 cgcgggctgg tgctcgagcc cgacgccgag gggctgctgc gggaggccgg gttcctgatc
     6421 gccgacgtgc tcgatcagat cggcctcgag gaacgcaccg accgggggct ggtcaaggaa
     6481 cggatccggg tcgagctccg gcgcttcttc cggaagcgct cggggcgccg gcccctcgtg
     6541 cttccggtga tcatggagat ttgaggtcgt ggctgctcgc tcgtccctgt cgcgccgctt
     6601 gagcgaattc ctgggcgtgg cgctgttcgc caccgcgctc atctggctca tcgcgctggt
     6661 cacctacgat ccgggcgacc ccgcctggtt cttccacgcc ggggccgacc ggcagccggc
     6721 gaacttcatc ggccgcgtcg gggcgttcgt cgccgaactg tcggtgcagg tcctcgggtt
     6781 cgcctcgtac ctggtgcccg tgctgctcgg ggtagtcggg tggacgctct tctggtgccg
     6841 gcgcgtcgag gcggtctaca ccaagctggt ggggacggcg ctgctcgccg gctgcctggc
     6901 ctcgctgctg cacctggcgc tcgagtcggt gaccctctcg gggcggcgcc tggccgcggg
     6961 aggctacatc ggagcgcaga tctcgagcga gctcacggcg tacttcagca agaccgggtc
     7021 gttcatcatc ctggtcacgc tgctcttcct ctcggtgatc ctcgccacgc agttctcctt
     7081 cgggcggatg ttctcggcga tcggcgacgc gatcttcgga tcggcccggc gcgcggtcgc
     7141 gcggttgcgc gagcggcgcg aggcgcgccg gcgcgagcgc gcccgccgcg aggtggtgcg
     7201 gaagcggatg gccgagagcc ccgaggccgt gaaggagatc gtcgagaagg cgcaacccga
     7261 gaaggtcgcc gcatccgcgc cctccaaggc ttccgccgcc gacacgtccc ggcccgcagc
     7321 cgtcgaggcc cctccgcgac cgcgcccgac gcccgccatc agccgccccc aggccgcgcc
     7381 cgccgcccgg aagccggcgg cgcccgccgc gccggtgccc gaagcggctg cggcctacga
     7441 gggacgcacg ccgatcgagc ggaagcggga aggcttcacg ccgccgccgc tcgcgctgct
     7501 cgacgcgccg cgcagcgagc gcaagatcga cgagcgcgag ctgatggaac tggcccgcca
     7561 gctcgaggag aagtgccacg agttcgcggt cgccggcagc gtcgtccaga tccaccccgg
     7621 gccggtggtc accaccttcg agttcaagcc cgacgccggc gtgaagtacg cgaaggtgac
     7681 ggggctcgcc gacgatctgt cgctggccct gaaggccgag tcggtactca tcgagcgaat
     7741 ccccggcaag tccacggtcg gcatccagat cccgaacacc agccgcgaac aaatctcgct
     7801 ccgcgagatg ctcgaatccg acctcttccg ccgctccggg tcgaagctca cgcttgccct
     7861 cggcaagacc attcacggcg aaccgttcgt cgctgacctg gccaccatgc cgcacctgct
     7921 catcgcaggc tcgacgggca ccggcaagtc ggtcgcgatc aacagcatgc tgaccagcat
     7981 cctgtaccgc gccacgcccg aggacgtccg gctgatcatg atcgacccca agcggctcga
     8041 gctggggatg tacgaggaga tcccgcacct gctgacgccg gtggtcgtcg atccgaaggc
     8101 cgcctccaac gcgctcgcct gggcggtgcg cgagatggaa gaacgctaca agacgctcgc
     8161 cgccgagggc gtgcggaaca tcgagcagta caaccgcaac atccgccacc agatggagga
     8221 gaagcgcgag gccgacgagg agggcgtgga gctgcgcacc ctcccctacg tcgtggtcgt
     8281 gatcgacgag ctggccgact tgatgatggt ggcgagcaac gaggtcgagg agtcgatctg
     8341 ccgcctcgcg cagatggcgc gcgccgtcgg catccacctc atcctcgcca cgcagcgccc
     8401 gtcggtggac gtgatcaccg ggctcatcaa ggcgaacctc ccggcgcgca tctcgttccg
     8461 cgtgtcctcg aaggtcgact cgcgcacgat cctcgacggc aacggcgccg agcaactgct
     8521 cggcaggggc gacatgctct acctcccgcc cgcctcctcc cggttgatcc ggctgcacgg
     8581 gccctacatc tccgagcagg agagcgcgcg cctggcaggc tacctgcgca agcaggggaa
     8641 gcctgtctac gacaccacca tcacggcgga cgagaagacc gccgacggcg tcgagttcga
     8701 gaaggacgat ctctacgacg aggcggcccg gatcgtcgtc gggtcagggc aggcctccat
     8761 ctcgtacctg cagcgccggc tgcggatcgg gttcagccgc gccgcgcggc tggtggacat
     8821 gatggagtcg gaggggctgg tctcgcccgc ctcgggcagc aagccgcgcg aggtgctggt
     8881 ggacaagggc tacttcgacg aggtcgacgc gcaactccgg tgatgatcct gacctcgcgg
     8941 cccgctcgcc tcgcgctgct cgccgccgtg gcgctggcgc tggccgttcc ggccggcgcg
     9001 cagcgcccgt ccgggcgggc agcggccccc gcgggacgcg agcggcccgc ccagatccgc
     9061 gacgtgaagc ggacccggct gaccgaggcg gtccgcatca cgatcgagct cgagcgcgag
     9121 gtcccgtacc gcagcgagcg gctcgacggc ccgccgcgcc tggtcttcga cttcctcggc
     9181 acgcggccgg ccgccacgct cgtccagggc tcactctcct accccgacga cgtcgtgcgc
     9241 cacatccggg tcgggcgcca ccccaacgac gtcacgcgcg tagtcctcga cctgacgggt
     9301 gtccagcgct acctggtgtt cgcgctctac gatccctacc gcctggtcat cgattgcgtg
     9361 cgcgccgtgc agccggctgt cacggcggcg gcacggccgc cggcgtcccc gcctgcggga
     9421 tcgaagcctg cggtccggga acccgggccg gccgctcccg agccgaggcc tgccctcccc
     9481 gccgtggcct cagcgcccct tcccccggag agagtcaccg agcacgagct tgccgccgcc
     9541 gacgtcgagc cgagcacggg gcccgtcctg atgttctcgc gccggttgcg cgtcgccttc
     9601 gtaccgaccg ccgaggcacc cgttcccgct ccgatcgagc tggaggcgga ggaggcgccg
     9661 cccgctgctg ccgctccgcc ctccgctccg ccgccctccg ctccgccgcc gcccgcaccg
     9721 gcgaaattgt ccgcgcccgc gacagcctcg agcggcgaaa agccgtcgac ccctgcgccg
     9781 ctcaagggcg ggttctcgct ggcacggcag ctcggcctgg gcgcgacgcg catcgtcatc
     9841 gaccccgggc atggcgggcg cgaccctggc gcgcaggcgc gcggcatctc cgaggcggcc
     9901 atcacgctcg acatcgcgct ccggctggag aagctgctca agggggcggg gatcgacgtc
     9961 gtcctgactc gccggaccga tcgctacgtg gcgctcgagg aacggacgga gatcgccaac
    10021 cgtgccgcgg gcgacctgtt cctctccatc cacgccaacg cgagccgcaa cacccgggcg
    10081 caaggcgtgg agagctacgt cctgaacttc gcgagcaacc cggaggccga ggccgtcgca
    10141 gcgcgcgaga acgcgtcgag cgagcggacg atgagcagcc tgaacgcgat cctgcaggcc
    10201 atcgccctca acaacaagct cgacgagtcg cgcggcttcg cggccagcgt gcagaacgcg
    10261 ctggtgcggc ggctgcgggc cacccggcga cagctcaagg atctcggcgt gaagcaggcg
    10321 cccttcgtgg tgctcatcgg cgcggagatg ccgagcgtgc tggtggaggt gtccttcatc
    10381 agccaccagc aggaggggcg tctgctgcgg acggcgacct accgccagag g
//
LOCUS       DAOPQX010000097        10411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7292_length_10411_cov_4.447277, whole genome shotgun sequence.
ACCESSION   DAOPQX010000097 DAOPQX010000000
VERSION     DAOPQX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7292_length_10411_cov_4.447277"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2375)
                     /locus_tag="PKK95_07125"
     CDS             complement(<1..2375)
                     /locus_tag="PKK95_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18021.1"
                     /translation="MAATIFADRFLARNECDAVDLATGFGVTLRPAQVSGRHAQEAWS
                     DRCAALASMCHPNLAALVDYGLAGRDGLFEAIEAPRPGTPWRSRDPETAAALCSVVAF
                     LHAHRRSAGSLSWSRLVDHDGRPVLLPDAATGSPWADEAAGDPGDTCAAREASRLARM
                     TGQVFGKVAAPRESRTAVGAAHGWAAVGRETVARLVEVLDTGEPARPRAIEVSLPPDG
                     RRKVLLSQIARESRLRGYVPVNSELLNARCAAAPGRDSLREALADRHVLLIHEVHAGG
                     GRSSLALFLVALGLSNTRPHVVALVVDAARPTLQAREQPAPYVRERHHSPALRLATAA
                     GRADPVAARAQAGLSAAGNLAARGRHAAAERLLRESLGAMSRREDRVGSGWAAVALGS
                     LLLARGRAAQAASSFEAARRHFDRADAVSAAGRCGLLLAHAWTDLGRLEEAEAAARAV
                     ALAARETGDAWLRAESTLALARCLFWQDRHGEGLQALEADERLALAPSRASRVAEVPP
                     PFESSGAPGGCFLAGARDQELARACLRARLGVALGDFVLAGRAAACARQRAGVRGDPA
                     GLAAAATAQLAVYGAIGDIEASGRARSAGLQAAHEAHDPLRALRLRIGWLEALAVSGG
                     RRDSSDAGSRLARLDRRALPWVVRHALERALARAATCGGGSIPGPLATAGRERAARLL
                     SPHSPSKVASVEWLLDDIVEVLALCQGPDDEESVLRAVIGRLRRRHHLATIACFAVEE
                     GSLVAVASDGSSPVPETAARRAVEVGRALGPEAFAARNEAAVPIRYAKAVAG"
     gene            2546..3781
                     /gene="aroC"
                     /locus_tag="PKK95_07130"
     CDS             2546..3781
                     /gene="aroC"
                     /locus_tag="PKK95_07130"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOC18022.1"
                     /translation="MLRFLTAGESHGPALVVILDGVPAGLAIDRAAIDAQLRRRQAGY
                     GRGRRMTIESDQVQVLSGVRRGTTLGSPIALMLPNRDAANWEHTMALDAEPPAAARGA
                     RRPAVTRPRPGHADLAGAIKYGHRDVRDVLERASARETAARVAAGAVARQLLERVGIA
                     IASHVVAIGGAHLELEEAVPFDRIAALPSDAPVRCVDERVAQAMMAAIDLAREDGDTL
                     GGAFEVVVRGVPPGLGSYTQWDRRLDGRLAQALLSIPSVRAVSIGTGWESAALRGSQA
                     HDVLVAAGDEGREPAAWGPSRPTNRAGGLEGGVSNGEEIRLTARMKPIPTLMRALPSV
                     DLETGLPAGAAVERSDACVVPAGGVIGEAVVALVLADAFMEKFGQDSMAEIEDNYTAW
                     RQRLRQRFARPGGTPGTDG"
     gene            3785..4294
                     /gene="def"
                     /locus_tag="PKK95_07135"
     CDS             3785..4294
                     /gene="def"
                     /locus_tag="PKK95_07135"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOC18023.1"
                     /translation="MVRPIVKYGAEVLHQPARLVEEITGEVAQLARDMVETMYRAPGV
                     GLAAPQVGVPLRLFVVDITLGHDPRGLITLVNPEWVAREGVQVEEEGCLSVPGFSASV
                     ARPQRAVVKGLDLEGREQTYEGQGLLARAFQHEMDHLEGKVYLDRLRGIRRSLLVRRI
                     HKRIKAGTW"
     gene            4297..5253
                     /gene="fmt"
                     /locus_tag="PKK95_07140"
     CDS             4297..5253
                     /gene="fmt"
                     /locus_tag="PKK95_07140"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOC18024.1"
                     /translation="MPTLRLVFFGTPAFAVPSLQALAGAGYPVLAAVTQPDKPAGRSR
                     RVVEGAVKQAASSMGIPVLQPDRLRDEAFQHQLAALGADLGVVAAYGKILPAAVLEAP
                     RLGMVNVHASLLPRWRGASPIHRAIMAGDAETGVSIMRVVQALDAGPVLDRVSRLIGP
                     DETAEQVEHDLARLGADLLLRVLASMERGEVREVPQAEEEVTYAPPLSREDGRIDWSR
                     PARAIHDQVRALQRWPHAFTTLAGSRIIVLETAVPDPGQEAVEAGVPPGTFLSLAGDA
                     LSVSTGTSPLHLRRVQAEGRRPVTGREFAAGARLKPGARLSS"
     gene            5250..6626
                     /gene="rsmB"
                     /locus_tag="PKK95_07145"
     CDS             5250..6626
                     /gene="rsmB"
                     /locus_tag="PKK95_07145"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOC18025.1"
                     /translation="MSGIAPARREAYAILRAVERGRADLPSAVARARDRLSDPRDRAL
                     ASEIAAGTLRWRAALDAVISAFTRKDVGRLDAEVLEVLRLGTYQLLHLERVPPSAVVH
                     DAVEQVRASGKRSASGLVNAVLRRVDRERHVLGLPARPADPASNAAGALDYLAITLSH
                     PRWLAERWLARFGFEATEAWLRFNNQPAPLTLRANTLSITRDELARQLAQVGVETAPT
                     RFAPDGLTVIRGNPFETALQEDGRFVIQDEASQLVGTFVGASPGERILDACAAPGGKS
                     LLMAGAVGPQGRVIAADLRERRVALLARSLQRTGAGSVRVVRADAAATLPFAPAFDVV
                     LLDAPCSGLGTIRRDPEIRWRRLPEHLPRFAAAQREMIRHAAAVVRPGGRLVYATCSS
                     EPEENEEVVASFLAAHPDFEAAGAGETGVAGGPAAAALDAAGHLRTWPFRHGLEAFFA
                     ASLRRRLA"
     gene            6672..7394
                     /locus_tag="PKK95_07150"
     CDS             6672..7394
                     /locus_tag="PKK95_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOC18026.1"
                     /translation="MALSTRVWSAGKLLLLVVALLTTYVLSAVVAARLTLNAREVRVP
                     ALVGATVNSASDSLGDLGLALKVEEARRVDPKVEAGHIAAQDPVPGSSARRGRSVRVW
                     LSSGQRAEAIPPLVGASARAALLRLERDGLALASLSEIRSNDYPPDTVIAQTPPGNTK
                     GDAVALLVNRGDRAAACLMPDLIGLPEAGASDLLRSAGFRVAVVARSPYPGVPPGVVL
                     RQSPQPGFEIAPGAAVSLEVSR"
     gene            7391..8080
                     /gene="rpe"
                     /locus_tag="PKK95_07155"
     CDS             7391..8080
                     /gene="rpe"
                     /locus_tag="PKK95_07155"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOC18027.1"
                     /translation="MKVQVAPSILAADFARLGAEVAAVERGGADYIHVDVMDGHFVPN
                     LTVGPAVVSSLKAVTRLPIDVHLMLEQPDRFVEAFIEAGAAMVSVHVEVSPHLDRTLS
                     LIKDFGARAGVVLNPATPVGSLEEVAGQVDYVLVMSVNPGFGGQAFLPSSASKVRRIR
                     ALLDEAGNQAPVEIDGGIDLDTVEQVVRAGAEILVAGSAIFHTPDPAAATRSLREAAG
                     RAAAAIGARRA"
     gene            8092..8505
                     /locus_tag="PKK95_07160"
     CDS             8092..8505
                     /locus_tag="PKK95_07160"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOC18028.1"
                     /translation="MPVPDHSPRQSVARLRVRYAETDKMGVVYYANYLVWFEVGRCEW
                     LRAAGLTYAGLEAEGIILPVVGAHCEYRQPARYDDEIEVLACGRLLSPVRVRFDYEVR
                     RAGADVLACGWTEHAAVDPNGRPRRLPLQVRRLFP"
     gene            8502..9452
                     /locus_tag="PKK95_07165"
     CDS             8502..9452
                     /locus_tag="PKK95_07165"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC18029.1"
                     /translation="MNVLITGVAGFVGSTLAGRLLGEGARVTGLDCFTDYYPRPLKEA
                     NLAPLLREPRFRFVESSIQEADLEKLLADATHVFHLAAQAGVRKSWGRDFSIYTTNNV
                     EATQVLLEAVVGRPIERLVYASSSSVYGDGTPLPMRETALPNPLSPYGVTKLAAEHLC
                     NLYHTNHGVPCVSLRYFTVYGPRQRPDMAFNRFIRAALAGEPISLYGDGGQTRDFTFV
                     ADAVAATAAAATRGVPGAVYNIGGGARVSVNHVLDVLGGIVGRRIDVRREGVQKGDMR
                     DTYADTSRARHDLGFSPSVTLADGLRAEYEWLAALPGGVR"
     gene            9458..10273
                     /gene="bamD"
                     /locus_tag="PKK95_07170"
     CDS             9458..10273
                     /gene="bamD"
                     /locus_tag="PKK95_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOC18030.1"
                     /translation="MHRKNTAARTAAAALLAAALALGSACGPKSAQPPPGGSQPDKFL
                     YDKGTEALNEKKWLRAREYFRQLVDNYPQSPLRPDAKLALGDTYLGENSLESLVLGLN
                     EFREFLSFYPTHKRADHAQYKLGMAHFQQMLGPQRDQTQTREAIAEFEVFFERYPNSP
                     LIDEVRKKYREARDRLSQSDYEVGVFYWRTRWYPGAVDRFREVLKADPEFTNRDGLYY
                     HLADSLIRMNLAAEAVPYLDRLLKEFERSEYLERAQKLLAEAKATLPADAVRK"
BASE COUNT         1443 a         3771 c         3807 g         1390 t
ORIGIN      
        1 ccggctaccg ccttcgcgta acgaatgggc accgcggcct cgtttcgcgc ggcgaacgcc
       61 tccggaccga gcgcccggcc cacctctacg gcgcgccttg ccgccgtctc gggaacgggc
      121 gacgacccgt ccgacgcgac ggcgacgagc gagccttcct ccacggcaaa gcaggcgatc
      181 gtggccagat ggtgccggcg cctcaagcgc ccaatcacgg cacgcaggac cgactcttcg
      241 tcgtcggggc cctggcaaag ggccagcacc tcgacgatgt cgtcgagcag ccattccact
      301 gacgccacct tcgatggcga gtgcggcgag aggagcctgg cggcgcgctc gcggccggcg
      361 gtggcgagcg gccccggtat ggagcctccg ccgcaggtgg cggcccgagc gagcgcccgc
      421 tccagggcgt gccgcaccac ccacgggaga gcccggcgat cgaggcgggc aagcctcgag
      481 ccggcgtcgg acgagtcgcg acgcccgccc gacaccgcga gagcctcgag ccatccgatc
      541 cgaaggcgaa gcgctctgag gggatcgtgg gcttcgtgtg cagcctgcaa cccggccgac
      601 cgcgcacggc cggacgcctc gatgtcgcca atcgcgccgt acacagccag ctgcgcggtg
      661 gccgccgccg cgaggcccgc cggatcgccc cgcaccccgg cacgctgccg cgcacaggcc
      721 gccgcgcggc cggccagaac gaagtcgcct agcgccactc caagacgcgc ccgaaggcac
      781 gcgcgagcca gctcctggtc gcgcgccccc gccaggaaac acccccctgg cgctcccgac
      841 gattcgaacg gcggcgggac ctctgcgacc cgggaagccc tcgacggcgc caacgccagc
      901 cgttcgtccg cctccaacgc ctggaggccc tcgccgtggc ggtcttgcca gaacaggcat
      961 cgtgccaggg ccaaggtcga ctcggcccgc agccaggcgt cgccagtttc ccgggcggcc
     1021 agcgcaacgg cccgggcggc ggcctcggct tcctcgaggc ggccgagatc cgtccacgcg
     1081 tgcgccagga gcaggccaca gcgcccggcc gcggacacgg cgtccgcacg gtcgaagtgc
     1141 cggcgtgccg cttcaaacga gctggcggcc tgtgccgcgc gtccccttgc cagcagaagc
     1201 gagccgagcg cgacggccgc ccagcccgag cccacccgat cttcgcgacg ggacatcgca
     1261 ccgaggctct cgcgcaggag tcgctcggct gcggcatgcc gtccgcgggc ggccaggttg
     1321 cctgccgccg acaggccggc ctgcgcacgc gccgccaccg gatcggcgcg gccagcggcc
     1381 gtcgccaggc gcagcgccgg cgagtggtgg cgctcgcgca cgtacggtgc cggctgctcg
     1441 cgcgcctgca gggtgggccg ggcggcgtcg accacgagcg cgaccacgtg cggccgggtg
     1501 ttcgagaggc cgagcgccac gaggaacagc gccaacgacg atctcccgcc gccggcgtgc
     1561 acctcgtgta tgaggagcac gtggcggtcg gcgagcgcct cccgcaggct gtcgcggccg
     1621 ggagccgcgg cgcaccgggc gttcaacagc tcgctgttga cgggaacgta cccgcggagg
     1681 cgcgactcgc gtgcaatctg ggacagcagc acctttcgac gcccgtcggg cggaagcgac
     1741 acctcgatcg cccgcgggcg cgccggctcc ccggtgtcga gcacctcgac gagcctggcc
     1801 accgtctcgc ggccgaccgc cgcccatccg tgagcggcac caacggccgt tcgcgattca
     1861 cgcggcgccg cgaccttgcc gaagacctgt cccgtcatcc gtgccaggcg cgacgcttcg
     1921 cgagccgcac aggtgtcgcc cgggtcacct gcggcctcgt cggcccacgg cgacccggtg
     1981 gccgcatcgg gcagcagcac cgggcgcccg tcgtggtcga cgagtcgcga ccaggacagg
     2041 ctgccggcgg agcgccggtg tgcgtgcagg aacgcaacca ccgagcagag ggcggccgcc
     2101 gtctcagggt cccgggaacg ccagggcgtg ccggggcgcg gcgcctcgat cgcctcgaac
     2161 agtccgtcgc gcccggcaag cccgtagtct acgagggccg ccaggtttgg atggcacatc
     2221 gacgcgagag cggcacagcg gtccgaccac gcctcctgcg cgtggcggcc ggagacctgg
     2281 gccggccgca gggtgactcc gaagccggtc gcgaggtcga ccgcgtcgca ttcgttccgc
     2341 gcgaggaatc gatccgcgaa aatggtcgcg gccatcgcgg tccaggtggt gcaagaagac
     2401 cgccgggaac ggcagcacgt ctccccgcac cctccctcgc aaggaatccg gccagtcttc
     2461 cggcgaacag ggcgcgcccc cgcttccggc ggagaacgcc ggggcgcccg ggggacgcag
     2521 aaacttcggc tagaatgagc cgatcatgct gcgatttttg accgccggcg aatcccacgg
     2581 cccggcgctc gtcgtcatcc tggacggggt gcccgccggc ctcgcgatcg accgggccgc
     2641 catcgacgcg cagctccggc ggcgccaggc cgggtacggg cgcggccgcc ggatgaccat
     2701 cgagtcggac caggtgcaag tgctgtcggg cgttcggcgc ggcacgacgt tgggcagccc
     2761 gatcgccttg atgctgccca accgcgacgc ggcgaactgg gagcacacga tggcgctcga
     2821 cgccgagccg ccggccgctg cccgcggcgc gcgccggccg gcggtgaccc gcccccggcc
     2881 cgggcacgcc gacctcgcgg gagcgatcaa gtacggccac cgggacgtgc gcgacgtgct
     2941 cgagcgcgcc agtgcgcgtg agaccgctgc ccgcgtggcc gccggcgcgg tcgcgcggca
     3001 gctgctcgaa cgggtgggca tcgccatcgc ctcgcacgtg gtcgcgatcg gcggggcgca
     3061 cctcgagctg gaggaggcgg tgcccttcga tcggatcgcc gccctgccgt ccgacgcccc
     3121 cgtgcgctgc gtggacgagc gcgtggccca ggcgatgatg gcggccatcg acctggcccg
     3181 cgaggacggc gataccctgg gcggggcatt cgaggtcgtc gtgcgcggcg tgccgccggg
     3241 cctgggcagc tacacgcagt gggaccgccg cctggatggc cgcctggcgc aggcgctgct
     3301 gtcaattccc tcggtgaggg ccgtctcgat cgggaccggc tgggaatcgg ccgccctgcg
     3361 cggatcgcag gcgcacgacg tgctggtcgc agccggggac gaggggcgcg agccggccgc
     3421 ctgggggccg agccggccga ccaaccgcgc cggcggcctc gaagggggtg tgagcaacgg
     3481 cgaggagatc cgcctgaccg cgcggatgaa gccgattccc acgctcatgc gcgcactgcc
     3541 ctcggtggac ctcgagacag gcctgcccgc cggcgccgcc gtcgagcgca gcgacgcctg
     3601 cgtggtgccc gctggcggcg tcatcggcga ggcggtggtc gcactggtgc tggccgacgc
     3661 gttcatggag aagttcggcc aggattcgat ggcggagatc gaggacaact acacggcctg
     3721 gcggcagcgg ctgcgccagc gattcgcgcg cccgggcggg acgccgggca cggacgggta
     3781 ggccatggtt cgcccaatcg tgaagtacgg cgcggaggtc ctgcaccagc ccgcgcgcct
     3841 ggtcgaggag atcacgggcg aggtggcgca gctcgcccgc gacatggtgg agaccatgta
     3901 ccgggcgccc ggagtgggcc tcgcggcgcc gcaggtcggc gtcccgctcc gcctcttcgt
     3961 ggtggacatc acgctgggcc acgaccccag gggcctgatc acgctggtca atcccgagtg
     4021 ggtcgcccgg gagggcgtgc aggtcgagga ggaaggctgc ctgagcgtgc ccggcttcag
     4081 cgcctcggtg gcgcggcctc agagggcggt cgtgaagggg ctcgacctcg aggggcgtga
     4141 gcagacctac gagggccagg ggctgctggc gcgagccttc cagcacgaga tggaccacct
     4201 cgagggcaag gtgtacctcg acaggcttcg cggaatcagg cgaagcttgc tggtacggcg
     4261 catccacaag cggatcaagg ctggaacctg gtagcgatgc caaccctgcg cctcgtcttc
     4321 ttcggcacgc cggccttcgc ggtgccaagc ctccaggcgc tggccggcgc cggctatccg
     4381 gtgctcgccg cggtgacgca gcccgacaag cccgcgggcc gcagccggcg cgtggtggaa
     4441 ggcgcggtca agcaagcggc ctcctccatg ggcatcccgg tactgcaacc cgatcggctt
     4501 cgtgacgagg ccttccagca ccagctggcc gcgctcggcg ccgacctcgg cgtcgtggcc
     4561 gcctacggga agatcctgcc ggccgccgtg ctcgaggccc cgcgcctcgg gatggtcaac
     4621 gtccatgcct cgctgctgcc ccgatggcgc ggcgcgtccc cgatccaccg ggccatcatg
     4681 gcgggcgacg ccgagaccgg cgtgtcgatc atgcgcgtcg tgcaggcgct cgacgccggc
     4741 cccgtcctcg accgggtcag ccggctcatc gggcccgacg aaaccgccga gcaggtcgag
     4801 cacgacctcg cacggctcgg ggccgaccta ctgctgcgcg tgctggcctc gatggagcgc
     4861 ggggaggttc gcgaggtgcc gcaggctgag gaggaagtca cctacgcccc accgctttcg
     4921 agagaggacg gccgcatcga ctggtcgcgg ccggctcgtg cgatccacga ccaggtgcgc
     4981 gcgctgcagc gatggccgca cgccttcacg acgctcgccg gctcgcggat catcgtcctc
     5041 gaaaccgccg tccccgaccc gggtcaagag gccgtcgaag ccggcgtccc acccggcacc
     5101 tttctctcgc tggcgggcga cgcgttgagc gtctcgaccg ggacaagccc gctgcacctc
     5161 cgccgcgtgc aggccgaggg gcgccgtccc gtcacggggc gcgagttcgc cgccggcgcc
     5221 cgcctcaaac ccggcgcgcg cctttcctca tgagcgggat cgcccctgcc cggcgcgagg
     5281 cctacgcgat cctccgggcg gtcgaacgcg gacgggccga cctgccttcg gcggtcgcgc
     5341 gcgcccgcga ccgtctgagc gacccgcgcg atcgtgccct ggcgtcggag atcgccgccg
     5401 ggacgcttcg gtggcgcgcc gcgctcgatg ccgtgatctc ggccttcacg cgcaaggatg
     5461 tcggccgcct cgatgccgag gtcctcgagg tgctccgtct ggggacgtac caactgctgc
     5521 acctcgagcg cgtgccgccc tcggcggtcg tacacgacgc ggtggagcag gttcgcgcga
     5581 gcgggaagcg aagcgcgtcg gggctggtca acgcggtgct gcgccgcgtg gatcgcgagc
     5641 gtcacgtgct ggggctgccc gcccgtccgg cagaccccgc cagcaacgcg gccggcgccc
     5701 tcgactatct cgccatcacc ctgtcgcacc cgcggtggct ggcggagcgg tggctggcgc
     5761 ggttcggctt cgaggccacc gaggcctggc ttcgattcaa caaccagccg gcgccgttga
     5821 cgctccgggc caacacgctg agcatcacgc gcgacgagct tgcgcgccag ctggcgcagg
     5881 ttggcgtcga gaccgcaccg acgcgcttcg cgccagacgg gctcaccgtc atccgcggca
     5941 accccttcga gacggccctg caggaagatg gacggttcgt gatccaggac gaggcgtcgc
     6001 agctcgttgg cacgttcgtc ggcgcctcgc cgggtgagcg catcctcgac gcgtgcgcgg
     6061 cacccggcgg caagtcgctg ctgatggccg gtgccgtcgg cccgcaaggc cgggtgattg
     6121 ccgccgacct gcgagaaaga cgggtggccc tgctcgccag gtcgctgcag aggacgggtg
     6181 ccgggtcggt gcgcgtggtc cgggcggacg ccgccgcgac gctgccgttt gccccagcct
     6241 tcgacgtcgt gctgctggat gccccctgct cgggcctggg gacgatcagg cgcgacccgg
     6301 agatccgctg gcgccgcctg cccgagcacc tgccccggtt cgccgccgcc cagcgcgaga
     6361 tgatccggca cgcggcggcc gtcgtccgcc cgggcggacg gctggtctat gcaacctgtt
     6421 cgagcgagcc cgaagagaac gaggaggtcg tggcctcgtt cctcgcggct caccccgact
     6481 tcgaggcggc gggtgcggga gagacgggtg tcgccggagg ccccgccgcc gccgcgctcg
     6541 acgccgccgg gcacctgcgc acctggccgt tccgccacgg cctcgaggcg ttcttcgccg
     6601 cgagcctgcg ccggcggctg gcgtgaccgg ctttggggac cggtgcgaat gtagtacatt
     6661 cgaacgcgac gatggccctc tccacccgcg tctggagcgc cgggaagctg ttgctgctcg
     6721 tggtggcgct cctcaccacc tacgtgctct cggcggtcgt ggcggcgcgg ctcacgctga
     6781 acgcccggga ggtgcgcgtg cctgcgctgg tgggcgcgac cgtcaactcc gcgagcgact
     6841 cactcggcga tctcgggctg gcgctcaagg tggaggaagc gcggcgggtg gatccgaagg
     6901 tcgaggccgg gcacatcgcc gcgcaggacc cggtcccggg ctcgagcgcc cggcgcggtc
     6961 gcagcgtgcg cgtctggctc agctcgggcc agcgagccga ggccatcccg ccgctggtgg
     7021 gcgcaagcgc ccgcgccgcg ctcctgcgcc tcgagcgcga cgggctggcg ctggccagcc
     7081 tctcggaaat ccgctcgaac gattaccccc ccgacaccgt gatcgcgcag acccccccgg
     7141 gaaacaccaa gggcgacgcg gtggcgctgc tggtgaaccg gggcgatcgc gccgcggcct
     7201 gcctgatgcc cgatctcatc gggctgcccg aggccggggc gtcggacctg ctgcgctcgg
     7261 cggggtttcg cgtggccgtc gttgcgcgca gcccgtaccc gggggtgccc cccggagtgg
     7321 tgctgcgcca gtcgccgcag ccgggcttcg aaatcgctcc cggagccgcg gtgtcgctcg
     7381 aggtgagccg atgaaggtcc aggtggcgcc gtccatcctc gccgccgact tcgcccgcct
     7441 gggggccgag gtggccgccg tcgagcgcgg cggcgccgac tatatccacg tggacgtgat
     7501 ggacgggcac ttcgtgccga acttgacggt ggggccagcg gtcgtctcct ctctgaaggc
     7561 ggtgacgcgg ctcccgatcg acgtgcacct gatgctcgaa cagcccgacc gcttcgtcga
     7621 ggcgttcatc gaagcggggg cggccatggt ctcggtgcac gtcgaggtgt cgccgcacct
     7681 cgaccggacg ctgtcgctga tcaaggactt cggggcccgg gccggcgtgg tgctcaaccc
     7741 ggcgacgccg gtgggctcgc tcgaggaggt ggccggccag gtcgattacg tcctggtgat
     7801 gtcggtcaac ccggggttcg gcggtcaggc tttccttccc tccagtgcgt ccaaagtcag
     7861 gaggatccgg gcgctgctcg acgaggcggg gaaccaggcg cccgtcgaga tcgacggggg
     7921 tatcgacctc gacacggtgg aacaggtcgt ccgggccggg gcggagatcc tggtcgcggg
     7981 ttcagccatc ttccacacgc cggacccggc ggccgccacc cgctcgctgc gtgaggcggc
     8041 aggccgtgcg gcggccgcga tcggcgcccg ccgggcatga gccgacagcc gatgcccgtt
     8101 cccgaccaca gccccagaca atcggtcgcc cgcttgaggg tccgctacgc ggaaaccgac
     8161 aagatgggcg tggtgtacta cgcgaactac ctcgtgtggt tcgaggtcgg ccggtgcgag
     8221 tggctgcggg cggcgggcct cacctacgcg gggctcgagg ccgaggggat catcctgccg
     8281 gtggtgggag ctcactgcga gtaccgccag ccggcgcggt acgacgacga gatcgaagtg
     8341 ctggcctgcg gccggctgct gtcgccggtc cgggtgcggt tcgactacga ggtgcggcgc
     8401 gcgggcgcgg acgtgctcgc gtgcggctgg accgagcacg cggcggtaga tccgaacggc
     8461 cggcctcgcc ggctgcccct gcaggtgagg aggctcttcc catgaacgtg ttgatcactg
     8521 gcgtcgcggg cttcgtcggc tccaccctgg ccggccggct cctcggtgag ggggcgcggg
     8581 tcaccggcct cgactgcttc accgactact acccgcggcc gctcaaggag gcgaacctgg
     8641 cgccgctgct gcgggagccg cgcttccgct tcgtggaatc atccatccag gaggccgacc
     8701 tcgagaagct tctcgccgac gccacgcacg tgttccacct ggccgcgcag gcgggggtgc
     8761 gcaagagctg gggccgcgac ttctccatct acaccaccaa caacgtggag gcgacgcagg
     8821 tcctgctcga ggcggtggtc ggccgcccga tcgagcggct cgtgtacgcc tcgagctcgt
     8881 cggtctacgg cgacggcaca ccgctgccga tgcgcgagac ggcgctgccc aacccgctct
     8941 cgccttatgg ggtcacgaag ctggcggccg agcacctgtg caacctgtat cacacgaacc
     9001 acggcgtgcc gtgtgtgtcg ctccgctact tcacggtgta cggccccagg cagcggcccg
     9061 acatggcgtt caaccggttc atccgcgcgg cgctggccgg ggagccgatc tccctctacg
     9121 gggacggcgg ccagacgcgc gacttcacct tcgtggccga cgcggtggcg gccaccgcgg
     9181 cagcggcgac caggggcgtg ccgggggccg tatacaatat tggaggaggc gcgcgcgtct
     9241 cggtgaacca cgtgctcgac gtgctcgggg gcatcgtcgg gcggcggatc gacgtgcggc
     9301 gcgagggcgt ccagaagggg gacatgcgcg acacctacgc ggacacgtcg cgcgcccggc
     9361 acgacctggg cttctccccc tcggtgacgc tggccgacgg gctgcgggcc gagtacgaat
     9421 ggctggccgc gctgcccggc ggggtgagat gacgacgatg caccgtaaga acacggcagc
     9481 caggacggcg gcggcggctc tgttggcggc ggcgctcgcg ctcggttcgg cctgcggccc
     9541 caagagtgcc cagccgcccc ccggcggcag ccagcccgac aagttcctct acgacaaggg
     9601 caccgaggcg ctgaacgaaa agaagtggtt gcgagcccgg gagtacttcc gccagctggt
     9661 ggacaactac ccgcagagcc cgctgcgccc cgatgcgaag ctggcgctgg gcgacaccta
     9721 cctcggggag aacagcctcg agtcgctcgt gctgggcctc aacgagttcc gcgagttcct
     9781 gtcgttctac ccgacgcaca agcgcgcgga ccacgcgcag tacaagctcg gcatggccca
     9841 cttccagcag atgctggggc cgcagcgcga ccagacgcag acccgggaag cgatcgccga
     9901 gttcgaggtg ttcttcgaac gctacccgaa cagcccgctc atcgacgagg tgcggaagaa
     9961 gtaccgcgaa gcccgcgacc gcctgagcca gtccgactac gaggtcggcg tcttctactg
    10021 gcgcacgcgc tggtacccgg gcgccgtgga ccgcttccgc gaggtgctga aggccgatcc
    10081 cgagttcacc aaccgcgacg ggctgtacta ccacctcgcc gactcgctga tccggatgaa
    10141 cctggcggcc gaggccgtgc cgtacctcga tcggttgttg aaggagttcg agcgaagcga
    10201 gtacctcgag cgggcgcaga agctgctggc cgaggcgaag gccactctgc ccgccgacgc
    10261 cgtccgcaag taggggcaac ccttgtggtt gccctttccg gcaccccgtg tggttgccct
    10321 ttcgggcggc ggcgcccggg cgacctaccg gttcgggatc tgggccgacc gctcgccggc
    10381 gccgcccttg acgaactgcc ggtagtactc g
//
LOCUS       DAOPQX010000098        10344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7352_length_10344_cov_4.599572, whole genome shotgun sequence.
ACCESSION   DAOPQX010000098 DAOPQX010000000
VERSION     DAOPQX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7352_length_10344_cov_4.599572"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="PKK95_07175"
     CDS             <1..1040
                     /locus_tag="PKK95_07175"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC18031.1"
                     /translation="HAVIAMAAMQLGKHVYVQKPLTHSVHEARVLTEAARKYKVASQM
                     GNQGHSGDGLRMVCEWIWAGALGEVREVHAWTNRPVWPQGVQMERPTDTPPPPAGMDW
                     DKWIGPAPMRPYHPAYHPEAWRAYWDFGTGSLGDMGCHILDPIFAALKLKYPTAVEGN
                     ISTFWHGFFKKTEPKNETYPASSIVRFRFPAREGMAPVVLTWWDGGLMPERPEELEEG
                     RKMGDEDGGCLFIGDKGKLMCGCYGRAPRLIPETKMQEFQRPDPTIPRIPGGEGGHEK
                     DWIRACKGGEPASSNFDYSGPLSEMVLMGNLAVRHPDRRLLWDGEKMEVTNDASANEY
                     VRRTYREGWSL"
     gene            1119..2015
                     /locus_tag="PKK95_07180"
     CDS             1119..2015
                     /locus_tag="PKK95_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC18032.1"
                     /translation="MSYLESDYRRTVTGEVRSRVLPGTSIEIVREVERPGRFRHVLFD
                     FDGTLSLIREGWPEVMVPMMVEALVATGTREPEEALRRHCHDFVMRLNGKQTIYQMIQ
                     LAAEVRARGGVPREPAEYKKEYHERLMERIGTRRNALAEGRAKPSDFLVPGALDLLAA
                     LRDRGLPMYLASGTDEQFVVEEAALLGLTPFFGPHVYGAIDDFKSFSKKMVIERILRE
                     NQVEGEALVAFGDGYVEIENVKAAGGVAVAVASDEAGRSGRPDEWKRDRLIGVGADVV
                     IPDFREWEPLVEYLLPAAQGRP"
     gene            2180..3139
                     /locus_tag="PKK95_07185"
     CDS             2180..3139
                     /locus_tag="PKK95_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18033.1"
                     /translation="MHKLFDRSRLRLKPLDERVHDVTRDVLVYPDSPYERSNHPALPI
                     LAARVRDARRNSRPVILMLGAHVLRRGAAPLLIDLMERGLVTHLALNGAGAIHDYELA
                     LAGSTCESVARYVRSGEFGLWTETGGINDAAICAQREGIGFGEAIGKAIVDGGFRFAE
                     TSLLAAGYRLGVPVTVHVAIGQDIVHEHPNFDAAATGAASYTDFLVFTESVSGLEGGV
                     LLNVGTAVMGPEVYLKALAMARNVAHQEGREIRHFTTGVFDLLPLDEEGLHREAPRTD
                     PRYYFRPYKTILVRTVADGGESHYVQGDHRYTVPTLYHLILEG"
     gene            3144..4190
                     /locus_tag="PKK95_07190"
     CDS             3144..4190
                     /locus_tag="PKK95_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC18034.1"
                     /translation="MNAERLQALVSRFPACRVAVLGDFFLDKYLDVDATLAEVSIETG
                     RTAHQVVSVRCSPGAAGTVVNNLAALGAGAIHTLGVIGDDGEGYELRRALEGTGCDVS
                     GLIVQPGRFTPTYLKPRDRRIPGLAGEHERYDTKNRRPTPPEVERRLLDHLGWLLPGL
                     DAVIVLDQVEEADCGAVTANVRAGLARLADAHPETLFWADSRRRIGLFRGLTVKVNEG
                     EAENAEGTASSGGRRVSDDELVRRAIARLRARTGKPVFVTAGARGTWVSDPEPLLVPA
                     VRVEGPIDPTGAGDSATAGAVLALCAGATLEEAALVANLVASITIEQLATTGTASPSD
                     LAPRLERWLGQRTA"
     gene            4260..4820
                     /locus_tag="PKK95_07195"
     CDS             4260..4820
                     /locus_tag="PKK95_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOC18035.1"
                     /translation="MQFAIDYLAALKKALDGLRPEDIGTAVSWMKKARDRGRFIYVCG
                     NGGSASIASQMVVDLLKGASYGHEKRFKVIALADSVATITAYANDVNYECVFAEQLRN
                     FAGEGDVLIAISGSGNSPNVLRAVEYANTIGCRTIALTQSGGGALKDLANLTLGVPAT
                     HMGRLEDSFFVLTHILMYAFMERAVE"
     gene            4915..5838
                     /locus_tag="PKK95_07200"
     CDS             4915..5838
                     /locus_tag="PKK95_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC18036.1"
                     /translation="MSPEPAWIGIDVGGTNMTGGLAAADGTLVAKVRRATDRRGGMAA
                     GLALISEIVFGLIDRAARDGQTVQRMGVGFGGPVDYVRGVVIKSHHVEGWENVPLRDE
                     LQRRFDVPAVVDNDANAGTLGEWRFGAGRGFDDLLYVNVGTGIGGGVVSSGRLVRGAA
                     NLAGEIGHTTIVRDGPPCTCGRRGCLEACASGDALARRGAEALGRAVTGKEVFALAGG
                     GNEAARRVVQAVVDDLAHGIGTAVGLLNPAAVIIGGGLSEAPEALFLEPLRRAIPRYC
                     LGEAARNLRVETAQLRYDAGVIGAVALALEG"
     gene            5887..6936
                     /locus_tag="PKK95_07205"
     CDS             5887..6936
                     /locus_tag="PKK95_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOC18037.1"
                     /translation="MAQIKGPAIFLAQFLDDKPPFNTLESITGWAKSLGFIGVQLPAW
                     DGRAIDLDKAASSKTYCDELKGRCNGLAITEISTHLQGQLVAVHPAYDTLFDGFAAPE
                     VRGNPKARTEWAVDQLKKAIKASHNLGLTAMPTFSGALLWQTVYPWPQRPAGLVDLGF
                     TELAKRWKPILDFADQHGVDLAYEIHPGEDLHDGNTFDMFLDKVGGHPRANMLYDPSH
                     YILQCLDYLENIDIYHDRIKAFHVKDAEFRPTGKGGVYGGYQDWKNRAGRFRSVGDGQ
                     VDFKQVFTKLTQYGYSSWAVLEWECCIKDGAQGAAEGAQFIKSMLIDVPQKAFDDFAG
                     GSADEASNKKILGLA"
     gene            complement(6997..7191)
                     /locus_tag="PKK95_07210"
     CDS             complement(6997..7191)
                     /locus_tag="PKK95_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18038.1"
                     /translation="MPWLAHELWHAVEIAGAPEVRDRASLLRLYQRIGEGFRAGGRME
                     METQRAQATQATVLNELRRR"
     gene            7383..>8845
                     /locus_tag="PKK95_07215"
     CDS             7383..>8845
                     /locus_tag="PKK95_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOC18039.1"
                     /translation="MRAHVVSLDLAVLAVYFVATMALGVGLSRRSRSVDGFTAAGRSL
                     PGWLTGLSILGTYISSITFLALPARAYAGNWNTFVFSLSIPLATWIAVRWFVPFYRRS
                     THVSAYHHLEARFGPWARAYASLCYLLTQLARMGTVMYLMALPISALLGWDIRAVILV
                     TGISVTIYALTGGLVAVIYADALQTIVLMGGALLAAVLAVAALPGGVGEVFRVAAEHG
                     KFSLGSLGPSLGQPTVWVVLLYGLIINLQNFGIDQNYVQRYIASRTDAEARKSVWLGG
                     LTYFPLSALFLFIGTALFAYYAANPGALPGELRDPGRADWVFPWYIVTAMPPGVGGLL
                     IAAIFAAAMSTVSTSLNSSATIILSDYYRRYVNREATERQSIRFLRVTTMVWGVAGTG
                     VALAMIRVRSALDAWWTLAGIFGGGMLGLFLLGFLSRRATGRAALGGVGAGVLLTLWM
                     TLSPRWAAFPEAWRSPFHEFLIIVFGTTAVLAVGWMLS"
     assembly_gap    8846..8945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8946..9896)
                     /locus_tag="PKK95_07220"
     CDS             complement(<8946..9896)
                     /locus_tag="PKK95_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HOC18040.1"
                     /translation="MRDIRVASVQFEHAPGDKDANFAKIRRFAGEAAERGVEIVAFPE
                     CCTTGYWFLRNLSREQLLKLAEPVPDGPSTRALLDLSRKHGMTIGAGLVELDRDNLHN
                     TYVVAMPDGNWRLHRKIHAFEGDDFVPGSAFTVFDTPHGCRVGVLICYDCNIVENVRI
                     TALMGADILLAPHQTGGCSTKNPHLMGLIERRVWEERHANPAAIEAELKGDKGRGWLM
                     RWLPSRAHDNGLFLVFSNGVGIDDDEVRTGNAMILDPYGRTLAETWAADDAMVVADLD
                     ASLLEHATGRRWITTRRPELYGPLIVPTGREKDTRAVRFDE"
     gene            complement(9981..>10344)
                     /locus_tag="PKK95_07225"
     CDS             complement(9981..>10344)
                     /locus_tag="PKK95_07225"
                     /inference="COORDINATES: protein motif:HMM:NF017380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOC18041.1"
                     /translation="LQSILLETYERPPADFEALLGMEGVGPRTVRALALASELVFGTP
                     ASTRDPARFAFAHGGKDGIPYPVDRQTYDRTIQVLGKALNRASVDRSEKVQAFKRLGR
                     FAAPGPGPVSHSSLDKER"
BASE COUNT         1575 a         3539 c         3604 g         1526 t
ORIGIN      
        1 cccacgccgt gatcgcgatg gcggccatgc agctcggcaa gcacgtctac gtgcagaagc
       61 cgctgaccca ctcggtgcac gaggcgcgcg tgctcaccga ggcggcgcgc aaatacaagg
      121 tggcgtcgca gatgggcaac caggggcact cgggcgacgg cctccgcatg gtgtgcgagt
      181 ggatctgggc cggcgccctc ggcgaggtcc gcgaggtgca cgcctggacc aacaggccgg
      241 tgtggccgca gggcgtccag atggagcggc ccaccgacac gccgccgccg ccggcaggga
      301 tggactggga caagtggatc ggcccggcgc cgatgcggcc ttaccacccg gcgtaccacc
      361 ccgaggcgtg gcgcgcgtac tgggacttcg gcaccgggtc gctcggcgac atgggctgcc
      421 acatcctcga cccgatcttc gccgcactga aactgaagta cccgaccgcg gtcgagggga
      481 acatctcgac cttctggcac ggcttcttca agaagacgga accgaagaac gagacgtacc
      541 cggcgtcgag catcgtgcgg ttccggttcc ccgcccgcga ggggatggcg ccggtcgtcc
      601 tgacgtggtg ggacggcggc ctcatgcccg agcggccaga ggaactggaa gagggccgca
      661 agatgggcga cgaggacggc ggctgcctgt tcatcggcga caagggcaag ctgatgtgcg
      721 gctgctacgg ccgcgccccg cgcctgatcc ccgagacgaa gatgcaggag ttccagcgcc
      781 ccgacccgac gatcccgcgc atcccgggcg gggaaggggg ccacgagaag gactggatcc
      841 gcgcctgcaa gggcggcgag ccggccagtt cgaacttcga ctactcgggc ccgctctcgg
      901 agatggtgct gatggggaac ctggcggtcc gccaccccga ccgccggctg ctgtgggacg
      961 gggagaagat ggaggtcacc aacgacgcct ccgcgaacga gtacgtcagg cggacgtacc
     1021 gggaggggtg gagcctgtag gaaggctcac ggctcacggc tcaaggccgt ctcagggccc
     1081 tgagccgttg gcctgagccc tgagccctga gccctctctt gtcctatctc gaatccgatt
     1141 accgccgcac cgtcaccggg gaggttcgct cgcgcgtcct ccccggcacc tccatcgaga
     1201 tcgtccgcga ggtcgagcgc cccggccggt tccgccacgt cctcttcgac ttcgacggca
     1261 cgctgagcct gatccgcgag ggctggcccg aggtgatggt gccgatgatg gtggaggcgc
     1321 tggtcgccac cggcacccgc gagccggagg aggcgctgcg ccggcactgc cacgacttcg
     1381 tgatgcggct gaacggcaag cagaccatct accagatgat ccagctggcc gccgaggtgc
     1441 gcgcccgggg cggcgtgccg cgcgagccgg cggagtacaa gaaggagtac cacgagcgcc
     1501 tgatggagcg catcgggacg cgccgcaacg cgctcgccga ggggcgcgcc aagccgagcg
     1561 acttcctcgt gccaggggcg ctcgatctgc tggcggccct ccgcgaccgc ggcctgccga
     1621 tgtacctggc cagcggcacc gacgagcagt tcgtcgtcga ggaggccgcc ctgctcgggc
     1681 tgacgccgtt cttcggcccg cacgtctacg gcgcgatcga cgacttcaag agcttctcga
     1741 agaagatggt gatcgagcgg atcctgcgcg agaaccaggt ggaaggagag gcgctcgtcg
     1801 ccttcggcga cggctacgtg gaaatcgaga acgtgaaggc ggccggcggc gtcgcggtcg
     1861 cggtggcaag cgacgaggca ggccgcagcg gacggccgga cgagtggaag cgcgaccgcc
     1921 tgatcggcgt cggtgccgac gtggtgatcc ccgacttccg cgagtgggag ccgctagtgg
     1981 agtacctgtt gccggccgct cagggacggc cgtgagcggg tgacgcgggg cggagggctc
     2041 ccgttcgcct ggagcgcgac acgcgtgaca agcgcttttc gcgcgaagcc gaacgggagg
     2101 agccccgcgg caggacccgc gaacatgcag ccgtccgaag agtgaccggc gaacgcgcgc
     2161 gaacgatgat cctgccgcca tgcacaagct cttcgaccga tcgcgcctga ggctcaagcc
     2221 gctcgacgag cgggtgcacg acgtgacgcg cgacgtgctc gtttaccccg acagccccta
     2281 cgagcgctcc aatcacccgg cgctgccgat cctggccgcc cgcgtgcgcg acgctcgccg
     2341 caacagccgg ccggtcatcc tgatgctcgg ggcgcacgtg cttcgtcgcg gcgcggcccc
     2401 gctgctcatc gacctgatgg agcgcggcct ggtgactcac ctcgcgctca acggggcggg
     2461 cgccatccac gactacgagc tggcgctcgc cggcagcact tgcgagagcg tcgcccgcta
     2521 cgtgcgcagc ggcgagttcg gcctctggac cgagacgggc ggcatcaacg acgccgccat
     2581 ctgtgcgcag cgcgagggga ttggcttcgg cgaggcgatc gggaaggcga tcgtcgacgg
     2641 gggcttccgg ttcgccgaga ccagcctgct cgccgccggc taccggctcg gcgtgccggt
     2701 caccgtccac gtcgccatcg gccaggacat cgtccacgag catccgaact tcgacgccgc
     2761 cgccaccggc gccgcctcct acaccgactt cctcgtgttc accgagagcg tgtcgggcct
     2821 cgaggggggc gtcctgctga acgtcggcac ggcggtgatg ggccccgagg tctacctcaa
     2881 ggcgctcgcc atggcgcgca acgttgccca ccaggagggc cgcgagatcc ggcacttcac
     2941 gaccggcgtg ttcgacctgc tgccgctcga cgaggagggg ctccaccgcg aggcaccgag
     3001 aaccgatccg cgctactact tccgccccta caagacgatc ctcgtgcgga cggtggccga
     3061 cggcggcgag agccactacg tgcagggtga ccaccggtac accgtcccca ccctctacca
     3121 cctcatcctg gaagggtgag cccgtgaacg cggaacgcct gcaggccctc gtgtcgcgct
     3181 tcccggcatg ccgggtcgcc gtcctcggcg acttcttcct cgacaagtac ctcgacgtgg
     3241 acgcgacgct ggccgaggtc tcgatcgaga cgggccgcac cgcccaccag gtggtgtccg
     3301 tccggtgcag cccgggcgcg gccggcacgg tggtcaacaa cctcgcagcg ctcggcgcgg
     3361 gcgcgatcca caccctcggg gtgattggag acgatgggga agggtacgag ctgcgaaggg
     3421 cgctcgaagg cacgggatgc gacgtcagcg ggctcatcgt tcagcccgga cggttcacgc
     3481 ccacctattt gaagccccgt gaccgccgca tccccggcct cgccggggag cacgagcgct
     3541 acgacacgaa gaaccgccgg ccgacgccgc cggaggtcga gcggcgcctg ctggatcacc
     3601 tgggatggct cctgccgggc ctcgatgccg tgatcgtgct cgaccaggtc gaggaggccg
     3661 actgcggcgc cgtcaccgca aacgtccggg ctggactggc gcggctggcc gacgcccacc
     3721 ccgaaaccct cttctgggcc gacagccgcc ggcgaatcgg cctcttccgc ggcctgacgg
     3781 tgaaggtgaa cgagggggag gcggagaatg cggaggggac ggcctcatcc ggcgggcggc
     3841 gtgtctcgga tgacgagctg gtgagacggg cgatcgcgcg gctaagggcg cggacgggca
     3901 agcctgtgtt cgtcacggcc ggcgcgcgcg ggacctgggt cagcgacccg gagcccctgc
     3961 tcgtgccggc ggtccgcgtc gaggggccga tcgatccgac gggcgccggc gacagcgcca
     4021 ccgcgggggc ggtgctcgcg ctctgcgccg gggccaccct tgaagaggcc gcgcttgtcg
     4081 caaacctggt cgcctcgatt acgatcgagc agctagccac caccggcacc gcgtccccgt
     4141 cggatctcgc gccccggctg gagcggtggc tgggccagcg gacagcgtag gggagacccg
     4201 ccgaagcctc gaggaggcga agcgggtggt cgccctgaca gctgacgtga ggaacttcga
     4261 tgcagttcgc gatcgactac ctggcggcac tgaagaaggc gctcgacggc ctccggcccg
     4321 aggacatcgg cacggcggtc tcgtggatga agaaggcgcg cgaccgcggc cgcttcatct
     4381 acgtctgcgg gaacggcggc agcgcctcga tcgcctcgca gatggtggtc gatctcctga
     4441 agggggcgtc gtacggccac gagaagcgct tcaaggtgat tgcactggcc gacagcgtgg
     4501 cgacgattac ggcgtacgcg aacgacgtga actacgagtg cgtcttcgcc gagcagctgc
     4561 ggaacttcgc cggcgagggc gacgtcctca tcgcgatcag cggcagtggc aacagcccga
     4621 acgtgctgcg cgcggtcgag tacgccaaca ccatcgggtg ccggacgatc gcgcttacgc
     4681 agtctggcgg gggggcgctg aaggacctgg cgaacctgac gctcggcgtg ccggcaaccc
     4741 acatgggccg cctcgaggac agcttcttcg tcctcacgca catcctgatg tacgcgttca
     4801 tggagcgggc ggtggagtag gcgagcggcg gagtatctgt cgcgaagccc tgagccctga
     4861 gcctgagcct tgagctgagc cgtgagcctt gaccctgagc ctttgcccta agccgtgagc
     4921 cctgagcctg cctggatcgg aatcgacgtc ggcggcacga acatgacagg cggccttgcg
     4981 gccgccgacg gcacgcttgt cgccaaggtc cgccgcgcta ccgatcgccg cggcggcatg
     5041 gcggccggcc tggcgctcat cagcgagatc gtcttcggcc tgatcgaccg cgccgcgcgg
     5101 gacggccaaa ccgtgcagcg gatgggcgtc ggcttcggcg ggccggtgga ctacgttcgc
     5161 ggggtcgtca tcaagagcca ccacgtcgag ggctgggaga acgtcccgct ccgcgacgag
     5221 ctgcagcggc gcttcgacgt gccggcggtc gtagacaacg acgcgaacgc cgggacgctc
     5281 ggcgagtggc ggttcggcgc cggccgcggc ttcgacgatc tcctttacgt gaacgtcggc
     5341 acgggcatcg gcgggggcgt tgtctcgagc gggcgcctcg tgcgcggcgc cgccaacctc
     5401 gcaggcgaga ttggccacac gaccatcgtc cgcgacggtc cgccgtgcac ctgcggccgc
     5461 aggggatgcc tcgaggcctg cgcgagcggc gatgccctgg cccggcgcgg cgccgaggcc
     5521 ctcgggcggg ccgtgacggg caaggaagtc ttcgcgctcg cgggcggcgg gaacgaggcg
     5581 gcccggcggg tcgtccaggc ggtcgtcgac gacctggccc acgggatcgg caccgcggtc
     5641 ggcctgctga acccggcggc cgtgatcatc ggcggcggcc tctccgaggc ccccgaagcg
     5701 ctgttcctcg agccgctgcg ccgggccatc ccccgctact gcctgggcga ggccgcgagg
     5761 aacctgcgcg tcgagacggc ccagttgcga tacgatgcgg gcgtcatcgg cgcagtggcg
     5821 ctggcgctgg aggggtgacg aagcagggct gaagccctgc tcgacgacgt acaaaaagga
     5881 gcacgtatgg cacagatcaa gggaccggcc atcttcctcg cccagttcct ggacgacaag
     5941 cctccgttca acaccctcga gagcatcacc ggctgggcga agtcgctcgg cttcattggc
     6001 gtgcagctgc cggcgtggga cggccgcgcg atcgacctcg acaaggccgc gtcctcgaag
     6061 acgtactgcg acgagctgaa ggggcgctgc aacggcctgg caatcaccga gatctcgacc
     6121 cacctgcagg ggcagctggt cgccgtgcac ccggcctacg acaccctgtt cgacggcttc
     6181 gcggcccccg aggtgcgggg taatccgaaa gcgcgcaccg agtgggcggt cgatcagctg
     6241 aagaaggcga tcaaggcgtc gcacaacctt ggcctgaccg cgatgcccac cttcagcggc
     6301 gcgctgctct ggcagaccgt ctacccctgg ccgcagcggc cggctggtct cgtggacctt
     6361 ggcttcaccg agctggcgaa gcgctggaag ccgatcctcg acttcgccga ccagcacggc
     6421 gtcgatctcg cctacgagat ccacccgggc gaagacctgc acgacggcaa caccttcgac
     6481 atgttcctcg acaaggtggg cggccatccg cgcgcgaaca tgctctacga cccgtcgcac
     6541 tacatcctgc agtgcctcga ctacctcgag aacatcgaca tctaccacga ccggatcaag
     6601 gcgttccacg tgaaggacgc cgagttccgc ccgaccggca agggcggcgt ctacggcggc
     6661 taccaggact ggaagaaccg cgcgggccgc ttccgcagcg tcggcgacgg ccaggtggac
     6721 ttcaagcagg tgttcaccaa gctcacccag tacggctact cgtcttgggc ggtgctcgag
     6781 tgggagtgct gcatcaagga tggcgcccag ggcgcggccg agggggcgca gttcatcaag
     6841 agcatgctca tcgacgttcc gcagaaggcg ttcgacgact tcgccggcgg ctcagcagac
     6901 gaggcgagca acaagaagat cctcggcctg gcctgatacc cactctggtg cccggccccc
     6961 gcggccgggc accgaaccct ctgtcgactc cgacgctcag cggcggcgca gctcgttgag
     7021 gacggtcgcc tgcgtcgcct gcgcccgctg cgtctccatc tccatcctcc cgcccgcccg
     7081 gaagccctcg ccaatccgct ggtacaagcg cagcaggctt gcgcggtccc tgacctccgg
     7141 cgcccccgcg atttccaccg cgtgccacag ctcgtgggca agccagggca ctgcaggcag
     7201 cggccagaat cgcgcacgcc gcccacctcc aaccactccg ccgccgccga ttcgtggccg
     7261 ccatcgcccc gtctcccggt tcgcggccgg ctcgattgcc ggctgcaccg ggtcaagcgg
     7321 aaacggcggg agaacaccgt catcgggcaa aggatgagat cacgatacaa tcccccccgt
     7381 cgatgcgcgc gcacgtcgtt tccctcgatc tcgccgttct cgccgtgtat ttcgtcgcca
     7441 ccatggccct gggcgtgggg ctgtcacggc gaagccgctc ggtggacggc ttcaccgcgg
     7501 ccgggcgcag ccttccgggc tggctgacgg gcctttccat cctcggcacc tacatcagca
     7561 gcatcacctt cctggcgctg ccggcgcggg cgtacgccgg caactggaac accttcgtct
     7621 tcagcctgtc catcccgctc gccacctgga ttgccgtgcg gtggttcgtc ccgttctacc
     7681 ggcgaagcac ccacgtctcc gcctaccacc acctcgaggc acggttcggc ccgtgggcgc
     7741 gggcgtacgc gagcctctgc tacctgctga cgcagctggc ccggatgggc acggtgatgt
     7801 acctgatggc gctgccgatc agcgccttgc tcggctggga catccgcgcc gtcatcctgg
     7861 tgaccggcat ctcggtcacg atctacgcgc tgacgggcgg cctggtggcg gtcatctacg
     7921 cggacgcgct gcagacgatt gtgctgatgg gcggggcgct gctcgcggcc gttttggcgg
     7981 tcgccgcgct gccaggcggc gtgggcgagg tgttccgggt ggcggccgag cacgggaagt
     8041 tcagcctcgg ctcgctcggc cccagcctgg gccagccgac ggtgtgggtg gtgctcctct
     8101 acggcctgat catcaacctg cagaacttcg gcatcgacca gaactacgtg cagcgctaca
     8161 ttgcgtcgcg gacggacgcc gaggcgcgca agagcgtgtg gctgggcggc ctgacctact
     8221 tccccctgtc ggcgttgttc ctcttcatcg gcacggccct cttcgcgtac tacgccgcta
     8281 acccgggggc gctgcccgga gagctgcgag accccggccg cgccgactgg gtctttccct
     8341 ggtacatcgt cacggccatg ccgcccggcg tcggggggct gctcatcgcc gcgatcttcg
     8401 cggccgcgat gagcacggtt tcgaccagct tgaactcgtc ggcgaccatc atcctcagcg
     8461 actactaccg gcgctacgtg aaccgcgagg cgaccgagcg ccagtcgatc cgtttcctgc
     8521 gcgtgacgac aatggtctgg ggcgtggcgg ggacgggggt ggcgctggca atgatccggg
     8581 tgcgaagtgc gctcgacgcc tggtggacgc tggccggcat cttcggcggc gggatgctgg
     8641 ggctgttcct gcttggcttc ctctcgcgcc gggcgaccgg gcgcgcggcg ctcgggggcg
     8701 tgggcgcggg cgttttgctc actctgtgga tgacgctgtc gccccgctgg gccgccttcc
     8761 ccgaggcctg gcgcagcccc ttccacgagt tcctgatcat cgtgttcggc acgaccgcgg
     8821 tgctggccgt ggggtggatg ctctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnctcgt cgaaccgcac cgcacgcgtg tccttctccc tgccggtcgg gacgatgagc
     9001 gggccataga gctcggggcg gcgcgtggtg atccaccttc ggccggttgc gtgctcgagc
     9061 agcgacgcgt ccaggtcagc caccaccatc gcgtcatcag cggcccacgt ctccgcgagg
     9121 gtgcggccgt aaggatcgag gatcatcgcg ttcccggtcc tcacctcgtc gtcgtcgatg
     9181 ccgacaccgt tggagaagac gaggaacagg ccgttgtcgt gcgcgcgcga ggggagccag
     9241 cgcatcagcc agccgcgccc cttgtctccc ttcagctcgg cctcgatggc cgcggggttc
     9301 gcgtgccgct cctcccagac gcggcgctcg atgagcccca tcaggtgcgg gttcttcgtg
     9361 ctgcagccgc ccgtctggtg cggggcgagc aggatgtcgg cccccatcag cgcggtgatc
     9421 cggacgttct cgacgatgtt gcagtcgtag cagatgagga cgccgacacg gcagccgtgc
     9481 ggcgtgtcga agacggtgaa cgcggagccg ggcacgaagt cgtcgccctc gaaagcgtga
     9541 atcttccgat ggaggcgcca gttcccgtcc ggcatcgcga cgacgtaggt gttgtggagg
     9601 ttgtcgcggt cgagctccac caggccggcg ccgatcgtca tcccgtgctt ccgcgagaga
     9661 tcgagaagcg cccgggtgga cgggccgtcg ggcaccggct cggccagctt cagcagttgc
     9721 tcgcgcgaca ggttccgcag gaaccagtag ccggtggtgc agcactcggg gaaggcgacg
     9781 atctccacgc cgcgctcggc cgcctcgccg gcgaaccgcc tgatcttggc gaagttggcg
     9841 tccttgtcgc ccggggcatg ctcgaactga accgacgcaa cacgaatgtc gcgcaacact
     9901 cgctcctttg tctggacctt gccgccgatg gttccacgat ccggggcgga ggcgcaaggg
     9961 aagagggaag cgccccgcgg ctatcgctcc ttgtcgagac tggagtgaga gacggggcca
    10021 ggccccgggg ccgcgaagcg gccgaggcgc ttgaaggcct gcaccttctc cgagcgatcc
    10081 accgaagcgc ggttcagcgc cttgccgagc acctggatcg tccggtcgta cgtctggcgg
    10141 tccaccgggt acgggatgcc gtccttgccc ccgtgggcga acgcgaagcg cgccgggtcg
    10201 cgggtgctgg cgggggtacc gaacaccagc tccgaggcca gcgcgagggc gcgcacggtc
    10261 cgcggcccaa ccccctccat ccccagcagc gcctcgaagt cggcgggcgg ccgttcgtat
    10321 gtctcgagca ggatcgactg gagg
//
LOCUS       DAOPQX010000099        10335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7363_length_10335_cov_4.988716, whole genome shotgun sequence.
ACCESSION   DAOPQX010000099 DAOPQX010000000
VERSION     DAOPQX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7363_length_10335_cov_4.988716"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PKK95_07230"
     CDS             complement(<1..783)
                     /locus_tag="PKK95_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOC18042.1"
                     /translation="MTTRLLAQKIVVAALGLALLVTAGISFRRSRNLAEPVVLGPGVT
                     SVKKLGEYFAAIRGTVNDCNLYFMEGREPGATMLVLGGSHAEEPAGRLAAWLLVENGA
                     VEKGRMIVVLSASRSASTMTRLGGAYPPDFTIGTPSGPRTFRMGDRWTNPLDQWPDPE
                     VFIHYPSRQELAYVDVRNLNRTWPGRPNGTLTEKTAFAFMEMIRREKVDVAIDLHEAE
                     LQYPVISTIVTHQRGQELATMASMMLTDNEGFKIGTEFSPLTL"
     gene            complement(791..937)
                     /locus_tag="PKK95_07235"
     CDS             complement(791..937)
                     /locus_tag="PKK95_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18043.1"
                     /translation="MVTLLYYALVAFVSLLLIVNLAKSRSWERDVLYVIVLVPFLLRL
                     FRLK"
     gene            complement(993..2285)
                     /locus_tag="PKK95_07240"
     CDS             complement(993..2285)
                     /locus_tag="PKK95_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18044.1"
                     /translation="MSMFTQAGIVLATMLAVYVAARLFRLSTELGMLAAAIGGALVGG
                     FGLPARHIAEGAATYLDINLIFVTATLFMNLLKESGGVAFVVRAILQRFHRSRALMLV
                     LLTILLLVPGALTGAGSVTVLVTGSLVAVVLHYMGIPKHRAAALVFVNAGLSAAAPPV
                     SLWAMMTAAGVNMPYVGFFWPLLLPCLVAGLLTSFILGWKGERTDAIKALAELPVPPA
                     GMSWLRVLLPFAVFFGLVWAGRQFPHSTPILGLPMMFAAAALASYLLSPVRLEFFRIS
                     RETIDQLLPLLATLTVVGILVQIMTLTGARGLLAVTAVTLPIAVVYATLFLVLPVSEA
                     VLMWGAAPVIGVPLVLLFNARGLDPIVALAGMSLIWPLGDALPPTAIIGRLTVEVVGF
                     KGPYGRFLRACLVPAILIIVFGTLMVIYSKRLGFLTGL"
     gene            complement(2296..2883)
                     /locus_tag="PKK95_07245"
     CDS             complement(2296..2883)
                     /locus_tag="PKK95_07245"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOC18045.1"
                     /translation="MFLFVRLPVLACLGAALVLGAAAPVQAQKAEAPILVTSCGQSPG
                     PIKLTVYMKRLQIAYDLKTDAAAKDLGAKPYKTLIVVTGSSLKGMGAAGVSIDDEVKR
                     VGALIDEAKKKGITVVGAHVEGMERRAKNAAPGDNSDEISIDAVCPKSSFLIVRKDGD
                     DDGRFTALSKKHNIPVVFFQKNTEIQDVLKNVFGR"
     gene            complement(2977..4497)
                     /locus_tag="PKK95_07250"
     CDS             complement(2977..4497)
                     /locus_tag="PKK95_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC18046.1"
                     /translation="MEHKRSGVLVAWLVPALTLLFHLASHQGYGYFRDELYYLANGRH
                     LAFGYVDHPPLVGLIAALVMNTIGSSLFAIRLLPAVAAAATVYVSGRLAGEMGGATFG
                     VVAAQLSAALVPAFLALFGFFSMNAFDILIWGILWWIAARYLRTRDERLWLAFGIVAG
                     VGLQNKISVLFAGFGIAVGLLAAGPREVFRRRWLWAGGAIAGLIFLPHLLWQAANGWP
                     TLEFIRNATANKNVAFSPLGYLAAQALNTLPAVPVWIAGLAALLAAPSFKPFRALGWA
                     WLAVLAVMLAINAKPYYLVPSFTVLLASGAVALERLGPSAAARAARAAALIVLVALGL
                     VAAPLAKALLPEDALVAYSAALGIEAPQEERHEMGRLSQHFADQHGWPELAASIAAVY
                     HRLPAAERERACIFAQNYGEAGAVDLFGPALGLPPAISGHNSYHLWGPRGCTGDIVVV
                     FGGDEREMRAAFQSLEHAATFTCTDCMPYENGTPIWIGRGLSPPLSELWPRLKAFN"
     gene            complement(4616..5932)
                     /locus_tag="PKK95_07255"
     CDS             complement(4616..5932)
                     /locus_tag="PKK95_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOC18047.1"
                     /translation="MSSRDSLRSFLLAALVMLSAPVLVQGQEAVPAERLAAREAFREA
                     RFGMFIHWGVYSMLGQGEWVMNNRDIQVPAYEALASSFNPIRFDAREWVALAKAAGVR
                     YITITSRHHDGFSMFASKASNYNIVDWTPFKRDPLKELANECRREGIKLFFYYSQLDW
                     RRSDYYPRGRTGNGTGRPDSGDWNAYLDFMDRQLEELLTNYGPLGGIWFDGMWDKPQA
                     PWRLDKTYALIHNLQPTALIVPNHHQPPMPGEDVQVFEQDLPGANTAGFNTKEIGKLP
                     LETCLTMNRSWGFNITDRSFKTTRELIGYLARAAGADANLLLNVGPRPDGTIQPEAVE
                     RLREVGKWLKVNAESIYGTRGGPITPRTWGVTTQRGSTVYVHVLDWPDRALTIPSWGA
                     RVTGARLLATGEKVGVTEVEGGIALTLPPAKPDDLDRVVVLETVTK"
     gene            complement(6207..7880)
                     /locus_tag="PKK95_07260"
     CDS             complement(6207..7880)
                     /locus_tag="PKK95_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOC18048.1"
                     /translation="MARAPGLVAPALQLRRLREITTVLVRYGFQDVVARLRLPGSGTV
                     GRVRRSRRDELPATRALRLRRALEALGPTFVKFGQALSTRADLLGPELIAELSLLQDS
                     VPPLPPGLAEAAIEAQLGAPVASLFREFDPVPIAAASIAQVHRAILPGGERVAVKVRR
                     PEIDGTIERDLAILAQLARLAERYLPDADLLQPSNLVSEFARSIRRELDLAREGRTFE
                     RFARNFEGDETVRFPGVYWTHTTNAVLALEFIDGVKALDIVGREGFDPALVARRGAAV
                     MLKQVLRHGLFHADPHPANLFILPGNVICMLDLGNVGHLDRRLRDAVAALVRAIVEED
                     AERLTHALLGIGRPLRPIDAGQLRRDVTELVDTYSGITFRNLQIGPLLQDALSIMRRY
                     RIQAPADLMLLVRAFVTIEGVGRQLDPTFKLIDEARPVVLQILRDRLMPAGVAARLGE
                     VGREAALALQDLPGDLLEIVSKAKDDRLQIQFVHRNLDQFVREMDRASNRLGFAIVIA
                     ALIVGSSLIVQGGGGALLFGLPALGLIGFVTAGILGLWLAVGIMRSGRL"
     gene            complement(7896..8258)
                     /locus_tag="PKK95_07265"
     CDS             complement(7896..8258)
                     /locus_tag="PKK95_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOC18049.1"
                     /translation="MVEALRKLLLAGLGTLDLTEEKARAVFNELVARGEMSEKDAREV
                     FATWSKRAGEQRGRMQQDIDAAVTKALSAMGLVRRSEVEALRGRIADLEQRLGMAPAE
                     AEAAAEPAPPPDVDRSGN"
     gene            8400..8636
                     /locus_tag="PKK95_07270"
     CDS             8400..8636
                     /locus_tag="PKK95_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC18050.1"
                     /translation="MPIYEYKCQSCGHQFEHITRGEVKPSCPKCQGGQLEKMLSVFAV
                     GAATPSSAAVPSDSPCARCGVPGGPGACGMAGMH"
     gene            complement(8633..9733)
                     /locus_tag="PKK95_07275"
     CDS             complement(8633..9733)
                     /locus_tag="PKK95_07275"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOC18051.1"
                     /translation="MSGPWPTPGQEDLLLAAFGPEPDARQAYLRLSRPSTTPDLAALW
                     PLVDRRWPPAETGRPPEGLAPYLTAWRQNRERLLSLEAVFEAFDREAIPFMLIKGGAL
                     LLRHYRDPGLRNMHDLDVLVPEATLEPAATVLAAAGFTPWRGVSPAGIRRRAHVRHAW
                     PFAGPEGTSVDLHWRPVARCFAPHVTRRFWEGCERVALGRRSARVPGPAEQLFQVCAH
                     ALQRDWDSRPRWVADALTVLRTPIDWERLGELAGGAAMRLRVAHALGYLRARFGAPVP
                     CGFLDSLAAAAERWERRERRLPLGSNASSAFDLFLWHVYTFRRLRPWHEPWAGLPAPL
                     AFSRYAAAALEVDRLSGIPAALWRWARGELRA"
     gene            complement(9730..>10335)
                     /locus_tag="PKK95_07280"
     CDS             complement(9730..>10335)
                     /locus_tag="PKK95_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18052.1"
                     /translation="LAEGREVGVAASVEEAVAPLTSWLDGRFAARTSHLVVHAGAAAL
                     DGAAVLVPGRSHAGKSTLTRALLQRGFRYYSDEYALIDPHGLLCPYPRALMLRDGRPT
                     ARPLLASECNGLPPDGPVRVALVLALSFEPDRGWAVERIPQGAMLLELLRNTPQAMAE
                     REDLVAGFARAVAGAACYRGTRGEAGEAASRIADLLVAVRG"
BASE COUNT         1685 a         3588 c         3646 g         1416 t
ORIGIN      
        1 caaggtcagc ggcgagaact cggtgccgat cttgaagccc tcgttgtcgg tcagcatcat
       61 cgaggccatc gtcgccagtt cctgtccgcg ctggtgcgtc acgatggtgc tgatcacggg
      121 gtactgcagc tcggcctcgt ggaggtcgat ggcgacgtct accttttcgc ggcggatcat
      181 ctccatgaaa gcgaaagcgg tcttctcggt gagggtgccg ttgggccgcc ccggccacgt
      241 gcgattcagg ttgcggacat ccacgtaagc cagttcctgc ctgctcgggt aatggatgaa
      301 gacctcgggg tcgggccact gatccagcgg gttcgtccag cggtcgccca tcctgaacgt
      361 gcggggaccc gacggggtgc caatcgtgaa gtcgggcgga taggccccgc ccaggcgggt
      421 catggtgctg gcgctgcggc tcgccgacag gacgacgatc atccggccct tctcgaccgc
      481 accgttctcg accaggagcc aggccgcgag gcgcccggca ggctcctcgg cgtgcgagcc
      541 cccgaggacg agcatcgtcg cgcccggctc gcgaccctcc atgaagtaga ggttgcagtc
      601 gttgacggtg ccgcggatcg cggcgaagta ctctccgagc ttcttcaccg acgtcacgcc
      661 cgggcccagg acgacgggct cggcgaggtt gcggctgcgg cggaagctga tgccggccgt
      721 gacgagcagc gcgaggccga gggcggcgac gacgatcttc tgggcgagga gccgggtggt
      781 catcgcgccc tcacttgagc cggaacaggc gcaggaggaa cggcaccagg acgatcacgt
      841 agagcacgtc ccgctcccag ctccgcgatt tggccaaatt gacgatcagg agcagcgaca
      901 cgaacgcgac cagcgcgtag tagagaaggg tcaccatcac gtgaaatccc gattccaggc
      961 cattccgcac ggccgccgcg cggccgcccc gattacagcc ccgtcaggaa cccgagcctc
     1021 ttgctgtaga tcaccatcag cgtcccgaag acgatgatca ggatcgccgg caccaggcag
     1081 gcgcgcagga accggccgta ggggcccttg aagccgacca cctccacggt gaggcgcccg
     1141 atgatggcgg tcggcggcag cgcgtcgccg agcggccaga tcaggctcat cccggccagc
     1201 gcgacgatcg gatcgaggcc gcgcgcgttg aacagcagca cgagcgggac gccgatcacc
     1261 ggcgccgcgc cccacatgag caccgcctcc gacacgggca gcaccaggaa cagcgtcgcg
     1321 tacacaaccg cgatcggcag cgtcaccgcc gtcaccgcca gcagcccccg ggcgccggtc
     1381 agcgtcatga tctgcaccag gatgccaacc accgtaagcg tcgcgaggag cggcagcagc
     1441 tggtcgatcg tctcgcgcga gatccggaag aactccaggc gcaccggcga cagcaggtac
     1501 gaggcgagcg ccgccgcggc gaacatcatc ggcaggccga ggatgggcgt gctgtgcgga
     1561 aactgccggc ccgcccacac gaggccgaag aagacggcga acggcagcag cacccgcagc
     1621 cagctcatgc ccgccggcgg aactgggagc tcggcgagcg ccttgatcgc gtcggtccgc
     1681 tcccccttcc accccaggat gaagctcgtg aggaggccgg caaccaggca cggcagcagc
     1741 aggggccaga agaaaccgac gtagggcatg ttgacgccgg ccgccgtcat catcgcccag
     1801 aggctgaccg gcggcgccgc ggcactcaaa ccggcgttga cgaacaccag cgcggcggcc
     1861 ctgtgcttcg ggatccccat gtagtgcagg acgaccgcca ccagcgagcc ggtcaccagc
     1921 acggtgacgc tccccgcccc ggtcagggcg ccgggtacca gcaggaggat ggtcagcagg
     1981 accagcatga gggcgcggct gcggtggaac cgctggagga tcgcccgcac cacgaaggcc
     2041 acgccccccg actccttgag gagattcatg aagagggtgg ccgtcacgaa gatcaggttg
     2101 atgtcgaggt aggtggcggc cccctcggcg atgtgccgcg ccggcaggcc gaacccgccc
     2161 accaacgctc ccccgattgc cgcggcgagc atccccagct cggtcgagag ccggaagagc
     2221 cgcgcggcga cgtaaacggc cagcatcgtg gcgaggacga tccccgcctg cgtgaacatg
     2281 ctcaccgggc ggctcctacc tgccgaacac gttcttcagg acgtcctgga tctcggtgtt
     2341 cttctggaag aagacgaccg ggatgttgtg cttcttcgaa agcgccgtga agcggccgtc
     2401 gtcgtcgccg tccttcctga cgatgaggaa gctcgacttc gggcagacgg cgtcgatgga
     2461 gatctcgtcg gagttgtcgc ccggcgcggc gttcttggcc cggcgctcca tgccctccac
     2521 gtgcgcgccg acgacggtga tccccttctt cttcgcctcg tcgatcaggg cgcccacccg
     2581 cttcacctcg tcgtcgatcg acacgccggc ggcgcccatg cccttcaggc tgctgccagt
     2641 gacgacgatc agggtcttgt agggcttcgc accaaggtcc ttggcggccg cgtcggtctt
     2701 caggtcgtac gcgatctgca ggcgcttcat gtacacggtg agcttgatcg gccccgggct
     2761 ctgcccgcac gaggtgacca ggattggcgc ctcggccttc tgggcctgga ccggcgccgc
     2821 ggcgcccagc accagggcgg cgcccaggca cgcgaggacg ggcaggcgta cgaagaggaa
     2881 catgggacac ctcacgggat tggtgtgttc tcagttgccg gttgtcgggt ttcagtactc
     2941 gagtcgtcag gtggggggcg acggggcagg atatcactaa ttgaacgcct tcagccgggg
     3001 ccacagctcg gagaggggcg ggctcaggcc ccgcccgatc cagatcggcg tgccgttctc
     3061 gtagggcatg cagtcggtgc acgtgaaggt ggcggcgtgc tcgagcgatt ggaaggcggc
     3121 acgcatctcc cgctcgtcgc cgccgaagac cacgacgatg tcgcccgtgc agccgcgagg
     3181 gccccagagg tggtagctgt tgtggcccga gatggccggc ggcagcccga gcgccggccc
     3241 gaagaggtcg accgcgccgg cttcgccgta gttctgcgca aagatgcagg cccgctcgcg
     3301 ctcggccgcg ggcaggcggt ggtagacggc ggcgatcgag gcggccagct cgggccaccc
     3361 gtgctggtcg gcgaagtgct gcgaaaggcg gcccatctcg tggcgctcct cctgcggggc
     3421 ctcgatgcca agcgccgccg agtaagccac cagggcgtcc tcgggcagca gcgcctttgc
     3481 cagcggcgcc gcgacgagcc ccagcgccac caacacgatc agcgccgccg cccgcgcggc
     3541 ccgcgcggcc gccgacggcc cgaggcgctc gagcgccacg gcgcccgagg cgagaagcac
     3601 ggtgaaggag gggacgaggt agtacggctt cgcgttgatc gccagcatca ccgccagcac
     3661 cgccagccag gcccagccga gcgcgcggaa cggcttgaag gacggcgccg ccagcagcgc
     3721 ggcaagcccg gcgatccaga ccggcaccgc cggcagcgtg ttgagagcct gggcggcaag
     3781 gtagccgagc ggcgagaacg cgacgttctt gttggcggtg gcgttccgga tgaactcgag
     3841 cgtcggccac ccgttcgcgg cctgccacag caggtgcggc aggaagatga gccccgcgat
     3901 cgcgccgccc gcccagagcc aacgccggcg gaacacctcc ctcggcccgg cggccagcag
     3961 ccccaccgcg atcccgaagc cggcgaacag gacgctgatc ttgttctgca ggccaacgcc
     4021 agccacgatc ccgaaggcga gccagagccg ctcgtcacgg gttcgcaggt agcgggcggc
     4081 aatccaccag aggatccccc agatcaggat gtcgaacgcg ttcatcgaga agaagccgaa
     4141 cagcgcgagg aacgcgggaa cgagcgccgc cgacagctgc gccgcaacca cgccgaacgt
     4201 cgcgccgccc atctcccccg cgaggcgccc ggacacgtac accgtggcag cggcggcgac
     4261 ggccggcagc agccggatcg cgaagaggga tgatccgatc gtgttcatca ccagcgcggc
     4321 gatgagaccg acgagggggg ggtgatcgac gtagccgaag gccaggtggc gcccgttggc
     4381 gaggtagtac agctcgtcgc ggaagtagcc gtacccctgg tggctggcca ggtggaacag
     4441 cagcgtcagc gctggcacga gccacgcgac gagcacgccg gatcgcttgt gctccatgaa
     4501 ccccacctat tcccagccct tagctccaag cccccagccc ctagctagcc cctagcccct
     4561 agcccctagc ccctagcccc tagcccctag cccctagccc ctaaccggtt agcccctatt
     4621 tcgtcaccgt ttcgagcacc accacgcggt cgaggtcgtc gggcttggcg ggcggcagcg
     4681 tcagcgcaat cccaccctcc acctcggtca cacccacctt ctcaccggtc gccagcagcc
     4741 gggcgcccgt cacgcgcgcc ccccaggacg ggatggtcag cgcgcggtcg ggccagtcga
     4801 gaacgtgcac gtagaccgtc gatccgcgct gggtcgtgac gccccaggtt cgcggcgtga
     4861 tggggccgcc gcgggtgccg tagatcgatt cggcgttgac cttcagccac ttccccacct
     4921 cgcgcagccg ctccacggcc tcgggctgga tcgtcccgtc ggggcgcggt cccacgttga
     4981 gcaggaggtt cgcgtcggcg ccggcggccc gtgccaggta cccgatcagc tcccgcgtcg
     5041 tcttgaagct gcggtcggtg atgttgaacc cccacgagcg gttcatcgtc aggcaggtct
     5101 cgagcggaag cttcccgatc tccttggtgt tgaagccggc ggtgttcgcc cctggcaggt
     5161 cctgctcgaa cacctggacg tcctcgccgg gcatcggggg ctggtggtgg ttggggacga
     5221 tgagggcggt gggctggagg ttatggatga gggcgtaggt cttgtcgagg cgccacggcg
     5281 cctgcggctt gtcccacatg ccgtcgaacc agatgccccc gagcgggccg tagttggtga
     5341 gcaactcctc gagctgccgg tccatgaagt cgaggtacgc gttccagtcg cccgagtcgg
     5401 gccgccccgt gccgttcccg gtgcggccgc gcgggtagta gtcggagcgc cgccaatcga
     5461 gctgcgagta gtagaagaac agcttaatcc cctcacgccg gcactcgttg gccagctcct
     5521 tgagggggtc gcgcttgaac ggagtccagt cgacgatgtt gtagttcgag gccttgctcg
     5581 cgaacatcga gaagccgtcg tggtgccgcg acgtgatggt gatgtagcgg acgccggcag
     5641 ccttggcgag agccacccac tcgcgcgcgt cgaagcggat ggggttgaac gacgaggcga
     5701 gggcctcata ggcgggcacc tggatgtcgc ggttgttcat cacccactcg ccctgcccga
     5761 gcatgctgta gacaccccag tggatgaaca tgccgaaccg ggcctcgcgg aaggcctcgc
     5821 gcgccgcgag ccgctcggcc ggaaccgctt cctggccctg cacgagaacc ggtgccgaca
     5881 acatcaccag cgccgcgaga aggaatgatc tgagagagtc tctgctgctc atggttgccg
     5941 ctgattctat tcctgcacgg ttcaaccaca aaggtcagaa aggacaggtt caccacagag
     6001 gcacagagga cacagagggc cgtcgtgctg gaggtgcaga aggtgcagaa ggtgccaaag
     6061 gtgctggtgc tgggggtgcc gaaggtgctg gtgctggggg tgccaaaggt gctggtgctg
     6121 ggggtgccaa aggtgctggt gctgggggtg ccgaaggtgc tggtgctggg ggtgccaaag
     6181 gtgctggtgc tggggtgcag gcgcggtcag aggcggccgc tgcgcatgat gccgacggcg
     6241 agccacaggc cgagaatgcc cgcggtcacg aagccgatga ggccgagagc cggcaggccg
     6301 aagagcaggg cgccgccgcc cccctgcacg atgagcgacg agcccacgat cagggcggcg
     6361 atgacgatcg cgaagccgag gcggttgctg gcgcggtcca tctcgcgcac gaactggtcg
     6421 aggttgcggt gcacgaactg gatctgcagc cggtcgtcct tcgccttcga aacgatctcg
     6481 aggaggtcgc cgggcaggtc ctgcagggcg agcgctgcct cgcgcccgac ctcgcccagg
     6541 cgcgccgcca cgccggcggg catcaggcgg tcgcgcagga tctgcagcac caccggcctt
     6601 gcctcgtcga tcagcttgaa ggtggggtcg agttgacgtc ccaccccttc gatcgtgacg
     6661 aaggcgcgca ccagcagcat caggtcggca ggggcctgga tgcggtagcg ccgcatgatc
     6721 gacagcgcgt cctgcagcag cggcccgatc tgcaggttgc ggaaggtgat gccggagtag
     6781 gtgtcaacca gctcggtgac gtcgcgtcgc agctggccgg cgtcgatcgg ccgcagcggc
     6841 ctgccgatgc cgagcagcgc gtgcgtcagg cgctcggcgt cctcctcgac gatcgcccgc
     6901 accagcgccg caaccgcgtc gcgcagccgg cgatcgaggt gcccgacgtt cccgaggtcg
     6961 agcatgcaga tcacgttgcc gggcaggatg aacaggtttg ccgggtgcgg gtcggcgtgg
     7021 aacaggccgt gccggagcac ctgcttcagc atgaccgctg ccccgcgccg cgccaccagt
     7081 gccgggtcga acccctcgcg cccgacgatg tcgagcgcct tgacgccgtc gatgaactcg
     7141 agggcgagga ccgcgttggt ggtgtgcgtc cagtacacgc ccgggaagcg gacggtctcg
     7201 tccccctcga agttgcgcgc gaaccgctcg aaggtccgcc cctcgcgcgc caggtcgagc
     7261 tcgcggcgga tcgaccgcgc gaactccgac acgaggttcg agggctgcag gaggtcggcg
     7321 tcggggaggt agcgctcggc cagccgcgcc agctgcgcga ggatcgccag gtcgcgctcg
     7381 atcgtcccgt cgatctcggg gcggcggacc ttcaccgcca cgcgttcgcc cccgggcagg
     7441 atggcgcggt gcacctgggc gatcgaggcg gccgcgatcg gcaccgggtc gaattcgcgg
     7501 aacaggctgg cgaccggcgc gccgagctgc gcctcgatgg ccgcttctgc aaggccgggc
     7561 ggcagcggtg gcaccgagtc ctgcagcagc gacagttcgg cgatcagctc gggccccaga
     7621 aggtcggcgc gcgtgctcaa cgcctggccg aacttgacga aggtgggccc cagcgcctcg
     7681 agggcgcggc gcagccgcag cgcgcgggtg gccggcagct cgtcgcgccg gctgcggcgc
     7741 acccggccca ccgtaccgct gccgggcagc cgcaggcggg ccaccacgtc ctggaaaccg
     7801 tacctcacca ggaccgtcgt gatctctcgc aggcggcgga gctgcagcgc cggggcgacg
     7861 agccctggag ccctggccat cggcgtcggc gcgggtcagt taccggagcg atcgacgtca
     7921 ggcggcggcg ccggctcggc agccgcctcg gcttccgccg gcgccatgcc aagccgctgc
     7981 tcgaggtcgg caatccgccc gcgaagcgcc tcgacctcgc ttcggcgcac cagccccatc
     8041 gctgacagcg ccttggtgac ggcggcgtcg atgtcctgct gcatgcgccc ccgctgctcg
     8101 ccggcgcgct tggaccaggt ggcgaagacc tctcgcgcgt ccttctcgct catctcgccg
     8161 cgggcgacga gctcgttgaa caccgcccgg gccttctcct cggtcagatc gagggtgcca
     8221 aggcccgcca gcaacagctt gcgcagcgct tccaccatga tgccctcctg ggtaggggta
     8281 cagggtacca cggggaaagg ctcggaagag gctatacaat aggctcaagg ctcaaggctc
     8341 acggctcacg gaacacgggc tgagggctga cggctttctg aatcccgaat cctgaagaga
     8401 tgccgattta cgaatacaag tgccagtcgt gcggacacca gttcgagcac atcacccgcg
     8461 gtgaggtgaa gccctcctgc cccaagtgcc agggagggca gctcgagaag atgctgtcgg
     8521 tcttcgccgt cggggcggcc accccttcga gcgcggccgt cccatccgac agcccgtgcg
     8581 cccgctgcgg cgtccccggg gggcccggag cctgcgggat ggccgggatg cactaggccc
     8641 gaagctcacc tctcgcccac cgccacaacg cggccggaat cccactgagg cgatcgacct
     8701 cgagcgcggc cgccgcgtag cgcgagaagg cgagcggcgc cggcagcccg gcccacggtt
     8761 cgtgccacgg gcgcaggcgg cggaacgtat acacgtgcca gaggaagagg tcgaacgccg
     8821 aggatgcgtt cgacccgagc ggaagcctcc gctcgcgccg ctcccagcgc tcggcagccg
     8881 cggccagcga atcgaggaac ccgcagggca ccggcgcccc gaagcgcgcg cgcaagtagc
     8941 caagtgcgtg cgcgacccga agccgcatcg cggcgcctcc cgccagctcg cccaggcgct
     9001 cccagtcgat gggcgtcctc agcacggtca gcgcgtcggc cacccagcgc ggacgcgagt
     9061 cccagtcgcg ctgcagcgcg tgggcgcaca cctggaagag ctgctcggcc gggcccggca
     9121 ccctggcgga acgccggccg agcgcgaccc gttcgcaccc ttcccagaag cggcgcgtga
     9181 cgtgcggcgc gaaacagcgg gccacgggcc gccagtgcaa gtcgacgctc gtcccctcgg
     9241 ggccggcgaa cggccacgcg tggcggacgt gcgcacgccg ccggatgccc gccggggaca
     9301 cgccgcgcca cggcgtgaag cctgccgcgg ccagtaccgt ggcggccggc tcgagcgtgg
     9361 cttccggcac cagcacgtcg aggtcgtgca tgttgcgcaa cccgggatcg cggtagtggc
     9421 gcagcagcag ggccccgccc ttgatcagca tgaagggaat ggcttcccga tcgaaggctt
     9481 cgaagaccgc ctcgagggac aggagccgtt cccggttctg ccgccacgcc gtcaggtagg
     9541 gcgcaagccc ctccggcggc ctgccggttt cggcaggcgg ccaccgccga tcgacgagcg
     9601 gccacagggc ggccagatcg ggcgttgtcg agggccggga cagccgcagg taggcttgcc
     9661 gcgcatcggg ttcggggccg aaggcggcca gcaggaggtc ctcctgccct ggcgtcggcc
     9721 agggcccgct cacccgcgca ccgccacgag caggtcggcg atgcgcgagg cggcctcgcc
     9781 ggcctccccg cgcgttcccc ggtagcacgc ggcacccgcc accgcccggg cgaagccggc
     9841 aaccaggtct tcccgctcag ccatcgcctg aggcgtgttc ctgagcaact cgagcagcat
     9901 ggctccttgc gggatgcgct cgaccgccca gccgcggtca ggctcgaacg acagcgccag
     9961 gacgagggcc acgcgaaccg gcccgtctgg cggaaggccg ttgcactcgc tcgcaaggag
    10021 tggccttgcc gtcgggcggc cgtcgcgcag catcagcgca cgcgggtagg gacagaggag
    10081 gccgtgaggg tcgatgagcg cgtactcgtc ggagtagtag cggaaccctc gctgcaggag
    10141 cgcccgcgtg agggtggact tccccgcgtg gctccgtccg gggaccagca cggccgcgcc
    10201 gtcgagcgcc gccgcgcccg cgtgcacgac gaggtgagac gtgcgcgcgg cgaaccggcc
    10261 atcgagccac gacgtcagcg gcgcgaccgc ctcctcgacg ctggccgcca cacccacttc
    10321 ccggccctcg gccag
//
LOCUS       DAOPQX010000100        10209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7477_length_10209_cov_4.296139, whole genome shotgun sequence.
ACCESSION   DAOPQX010000100 DAOPQX010000000
VERSION     DAOPQX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10209
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7477_length_10209_cov_4.296139"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="PKK95_07285"
     CDS             <1..1755
                     /locus_tag="PKK95_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOC18053.1"
                     /translation="DLAGVAAVALGPTSPEWLRESSDRYKLELERAERLFAARRYQPA
                     REAFELLRQVARGGDAELTAMRVAECDHYLGRYQQARDGLAPFLDKASRKAEARFFYL
                     TATRGLKRYDEYVRLARELVRDFPDSSWAHETLNNLGTHHILQDEDEEAARVFAELYK
                     ASPKGRHAPRAAWKAGWWAYKNGRYDEAAHFFEDAAAGFPRSDYRPSYLYWAARAREQ
                     AGDRAGAASVYRIVHADYQQSYYGRLTGTRLSKLGVPLEADSEATAKPGTRGAPKAAA
                     VVTAAGEEEGATAAGDAVAAAAGANLPPPPNAALIRLLIGLELYDLAREEVLYAQRTT
                     GNLPLLDATLAWTYHKENDLRRGIIHMKRAYPQFLSAHGTTLPDDLLRVIYPMDYWPL
                     ISKYSKQHGLDPFLVAALINQESSFQADARSRANAIGLMQVLPSTGRRLARQQRLRFT
                     TASLTRPEYNIRLGTAFFADLSRRLGGVHLALASYNAGAHRVATWQAERPGLERDEFI
                     DDIPFPETQTYVKKILGSADDYRALYKSLAAGTAPGARPSASATKPGDAAKASAKKPA
                     AKAPPKKAPAKPATRKKK"
     gene            1892..4495
                     /locus_tag="PKK95_07290"
     CDS             1892..4495
                     /locus_tag="PKK95_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18054.1"
                     /translation="MGLSAWVSLGTLTVTGAGPGAGRVALLPPWWVLITAAAAALAIL
                     LPLARRGVPAWPLATLAIILVPWLPLPPAGALLMWAGPLTAWVWVAALAAWACRWAAA
                     PAAPRSQVLAFVLALALFGGAAWQLREARPGGDEPHYLVIVQSLLKDGDLRIENNHER
                     RDYAEYFGGVIGPHFLKRGTDGQIYSVHAPGLPVVVAPAFALGGYQAVVALLVVVAAL
                     GMLLTWRVTWLLTGDAGAAWFAWGTSLGAPFFFHSYAVYPDALGAVLVATGILALVRL
                     ETRRPLPPWRWVLHGAALALLPWLHTRFAVAAAVLGASIALRLAAGVRFPGQRSRVHG
                     DAAAGEGLGGRTLFAFLVIPAISAVAWFGFFRIIWGTFSPTAPYGTYTQTAASNILRG
                     LPGLLLDQQFGILPNAPACLCALAGFWPLARRRPRLAVELSLLVAAYLAAVSSYHMWW
                     GGWSAPARFAVPVMLALAVTAGAYWAAARRAGRAAGAALLGLTLLITGTMTFGGGRSI
                     FNSRDGFARWLDRAGPNVDLPRALPSFFRDGRALAFGQAAAWLLAFVLALLVLRLVVR
                     AAERRPGANVRLRLATAVPALAALALGGATTVGWRLGGANGVAPLAGQLALLQAYDPE
                     RLPIRIGAPARLALKQPEGRPAAEGVLLALGPLPAGTYRLEPASLPAARGTTTVRLGR
                     DERPLATWDLDGRAERTLELPAGAAFLAIEGDEDARATAPGIALRPVTLIPPGLRASP
                     GFARHAAAYGPVIVYAFDGRAWLEEPGLWVRGGVEVPLVFAKGGARRLEVLLRNGPLP
                     NKVEVDGGTWRLDLQLAPRETRAIEVPLWGTPAAALVRVKAERGFRPSETAPGNRDER
                     YLGVWMEVPRP"
     gene            complement(4503..5339)
                     /locus_tag="PKK95_07295"
     CDS             complement(4503..5339)
                     /locus_tag="PKK95_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18055.1"
                     /translation="MRNTIRLAACLTAALAVGASTASAQYKQYPTSQPATGERYHVEA
                     SFGLWNPTPTILVSSESLGIVGTEIDGVNDLGMEKTRFKEFRVVLRPGKKHKLRFQYI
                     PILFEAEQPRLERAIVFNGIKYNVGVPVNSSLDWKAYRFSYEYDFLYRDRGFLGFVVD
                     AKYTDVEVNLESPVLPDPEYTRARAPIPALGLIGRIYPLGNVSLTAEFTGFKLPASVS
                     DEKDRYDGKYLDFDIYGTLNLTNNFGVQAGYRSIDVMYKVKSDNGDFKLKGLYFMGVA
                     RF"
     gene            complement(5424..7244)
                     /locus_tag="PKK95_07300"
     CDS             complement(5424..7244)
                     /locus_tag="PKK95_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOC18056.1"
                     /translation="MHALYLLVPILCILVIAYRYYSAFITARLLVLDDTRRTPAHTQY
                     DGHNYYPTSRWVLFGHHFAAITGAGPLVGPVLAAQFGYAPGLLWLVAGVVLAGAVHDL
                     IILWASTRRGGRSLAEIARIEIGPVAGLTTGLAILFIVVIALAGLGLVVVNALSQSPW
                     GTFTIGATIPLALFMGFYMYRFRRGKIAEATVIGVIGLLAAVVFGKPLAATAFGQYFT
                     LTHQQLVIAMAAYGFLASVLPVWMLLAPRDYLSAFMKIGTILLLVAGVIIVNPELHMP
                     AVTSFAAGGGPIIKGALFPFVFITIACGAISGFHGLVGSGTTPKMIDKESDIRPIGYG
                     AMLIEGLVGIVALIAATALHPGDYFAINVPAAAFATLGIPPVNLEALEAAVGETVAGR
                     TGGAVSLAVGMAQIFSALPGMKGLMDYWYHFAIMFEALFILTTIDAGTRVARFLVQEF
                     FGRFYPPFARTDWMPGTVISTGAVVVAWAYFIWTGSIGTIWPMFGISNQLLAVIALLI
                     GTTILVNLGRARYAWVTVVPLVFLATTTLSAGFLSVRDNFWPMAVGPDPALHVQGYLN
                     SIMTVVMMACVVVIVVASVRRWMAPAGSAAPADNTMQGTA"
     gene            7274..9025
                     /locus_tag="PKK95_07305"
     CDS             7274..9025
                     /locus_tag="PKK95_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18057.1"
                     /translation="MRPARRLLPYLLAYRRPLALGLASVVATTAVSLVSPWVLKYAID
                     DLTAGVTRGKLASYAALLLGLGVLSGAFRFLMRRLIVGVSRHIEYDLRNQFFATLQRQ
                     PAAYFQSRRTGDLMSRATSDLNAVRTVVGPAIMYTTNTAILFVVAIALMASIDWRLTL
                     MALVPLPAVSVAVRFFGQAIHHRFERIQAQLSDLSAVVQETLAGVRVVRAYRQEQAEI
                     ERFARSNREYLERNRILIRLQSLFFPSLSLLLGLGTLLILWLGGRAAVAGRITIGEFV
                     AFNAYLAMLAWPMIAFGWVTNMVQRGLASWERLLEVLDAEPSIAEPPPGRLVRLPEIR
                     GEIEFRELSFAFGEQVVLRDVSVRIEAGQTLALVGATGSGKSALISLLPRLYDPPAGT
                     VFLDGVDVRNLSLAQLRAAIGFVSQEPFLFSDTLANNIAFGSERPAAEQMDRVEWAAG
                     IAQLDKDVAGFPKGYNTMVGERGITLSGGQKQRTALARALVADPKVLILDDSLSAVDT
                     DTEERILSGLRGVMRERTSIIVSHRVSTVREADLILVLEGGRVVERGTHDELVRLDGF
                     YAGLYRKQLLEEELAAS"
     gene            9081..>10209
                     /locus_tag="PKK95_07310"
     CDS             9081..>10209
                     /locus_tag="PKK95_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOC18058.1"
                     /translation="MAHEEEVLGKAYDARLMRRLLGYMRPYAGHVAVAFAAIVGASLL
                     QLAQPYLVKVAIDRYIAQGNAAGLDLIAGAFLAILLAAFGLEFLQTYVLQLVGQRIMY
                     DLRVQVHRHLQALDLSFYDRNPVGRLMTRVTTDVDVLNDLFASGVITVFGDVVTLTGI
                     MVVLLVMDWRLALAAFSVLPLIVLVTQWFRRHVRESYRTVRVLIARINAFLQENLGGM
                     ATVQLYRREAAQFRKFDEIDGAHRDANLDSIFYYAVFYPAIEVIGALAVALIIWYGGG
                     LVLAGALTLGSLVAFIQYSQRFFRPISDISEKFNLLQAAMASSERIFRLLDTPPAIVS
                     PARPAARAVTRGKIEFRDVGFAYHPGEPVLRGVSFVVEPGEQ"
BASE COUNT         1456 a         3504 c         3667 g         1582 t
ORIGIN      
        1 gatctcgcgg gggtcgcagc cgtggctctc gggccaacga gcccggagtg gctgcgcgag
       61 tcgtctgacc gctacaagct cgagctggag cgcgccgagc ggctcttcgc ggcgcgccgc
      121 taccagccgg cccgcgaggc gttcgaactg ctgcgccagg tggcccgcgg tggcgacgcc
      181 gagctcaccg cgatgcgcgt ggccgagtgc gaccactacc tggggcggta tcagcaggcg
      241 cgcgacggcc ttgctccctt cctcgacaag gcgtcgcgga aggccgaggc gcgcttcttc
      301 tacctcaccg ccacccgcgg cctgaagcgc tacgacgagt acgtccggct cgcgcgcgag
      361 ctggtccgcg acttcccgga cagctcgtgg gcgcacgaga cgctcaacaa cctcgggacc
      421 caccacatcc tccaggacga ggacgaggag gcggctcgcg tcttcgccga gctgtacaag
      481 gcctccccga agggacgcca cgcgccgcgc gccgcgtgga aggccgggtg gtgggcgtac
      541 aagaacggcc gctacgacga ggcggcgcac ttcttcgagg acgcggccgc cggcttcccc
      601 cgctccgact accgtccgtc gtacctctac tgggcggccc gggcgcgcga gcaggcgggc
      661 gaccgtgccg gcgcggccag cgtctaccgc atcgttcacg ccgactacca gcagtcgtac
      721 tacggccgcc tgaccggcac ccggctctcg aagctgggcg tgccgctcga ggccgactcc
      781 gaggcgacgg cgaagcctgg cacgcgcggc gcgccgaagg cggccgccgt cgtgacggcg
      841 gccggcgagg aagagggcgc gacggcggcc ggcgacgcgg ttgcggccgc ggccggcgcc
      901 aatctgccgc cgccgcccaa tgccgcgctg atccgcctgc tgattgggct cgagctgtac
      961 gacctggcgc gcgaagaggt gctctacgcg cagcggacga cgggcaacct gccgctgctc
     1021 gacgccaccc tggcctggac gtaccacaag gagaacgacc tgcggcgcgg gatcatccac
     1081 atgaagcggg cgtacccgca gttcctctcg gcgcacggca cgacgctgcc cgacgacctg
     1141 ctccgcgtca tctacccgat ggactactgg ccgctgatca gcaagtactc gaagcagcac
     1201 ggcctcgacc cgttcctcgt ggcggcgctg atcaaccagg agtcgtcttt ccaggcggat
     1261 gcgaggtcgc gggcgaacgc gatcggcctg atgcaggtcc tcccctcgac gggccggcgt
     1321 ctcgcccggc agcagcggct gcgctttacc acggcatctc tgacgcggcc cgagtacaac
     1381 atccggctcg gcaccgcgtt cttcgccgac ctgtcgcgcc ggctcggtgg cgtgcacctg
     1441 gcgctcgcca gctacaacgc cggcgcacac cgcgtggcaa cctggcaggc ggagcggccg
     1501 gggctcgagc gggacgagtt catcgacgac atcccgttcc ccgagaccca gacgtacgtg
     1561 aagaagatcc tcggctcggc agacgactac cgggcgctct acaagagcct cgccgcgggc
     1621 acggctcccg gcgccaggcc ttccgccagc gcgacgaagc ccggggacgc ggcgaaggcg
     1681 tccgcgaaga agccggccgc gaaggctccc ccgaagaagg cgcccgcgaa accggccaca
     1741 cggaagaaga agtagcggtc tggtgtagca tctcccagac gcgggaggcg cgacactgca
     1801 ggcacgcaca ccatctggga cgggcgcgga cggtgcgaga ctggcggcgc gagggcggac
     1861 aaccctcgaa gccgtcaccg tttctgcgat cgtgggcctt tcggcctggg tctcgctcgg
     1921 cacgctcacc gtgaccgggg cgggtccggg tgctggccgc gtcgcgctgc tgcctccctg
     1981 gtgggtcctc atcacggcgg cggcggccgc gctcgccatc ctgctgccgc tggcccgccg
     2041 cggcgtgccg gcctggcccc ttgccaccct cgcgatcatc ctcgtgccct ggctgccgct
     2101 gccgcccgcg ggggccttgc tgatgtgggc ggggccgctc acagcctggg tgtgggtggc
     2161 cgcgctggcc gcgtgggcct gcaggtgggc ggcagccccg gcggcgccgc ggtcgcaggt
     2221 gctcgccttc gtgctcgcgc tcgcgctctt cggcggcgcg gcgtggcagt tgcgcgaggc
     2281 ccgccccggc ggcgacgagc cgcactacct ggtcatcgtc caaagcctct tgaaggacgg
     2341 cgacctccgc atcgagaaca accacgaacg ccgcgactac gccgagtact tcgggggcgt
     2401 gatcgggccg catttcctga agcgcggcac ggatggacag atctattccg ttcacgcccc
     2461 gggactgccc gtcgtcgtag cgccggcgtt cgcgctgggc ggctaccagg cggtcgtcgc
     2521 gctgctggtg gtggtggccg cgctcgggat gctgctgacg tggcgagtca cgtggctgct
     2581 caccggagac gccggcgcgg cgtggttcgc gtggggcacg tcgctgggag cgccgttctt
     2641 cttccactcg tacgcggtgt accccgacgc tctcggtgcg gtgctggtgg cgaccggcat
     2701 cctcgcgctc gtgcgcctcg agacgcgacg cccgctgccg ccgtggcgat gggtgctgca
     2761 cggcgcggcg ctcgccctcc ttccttggct tcacacgcgc ttcgcggtgg cggcggcggt
     2821 gctcggggcc tcaatcgccc tgcggctcgc cgcaggcgtg aggttccctg gccagcgtag
     2881 ccgggtgcac ggagacgccg cagccggcga aggccttggt ggccgcacgc tgttcgcttt
     2941 cctcgtcatc cccgcgatca gcgcggtcgc ctggttcggg ttcttccgga tcatctgggg
     3001 aacgttcagc ccgacggcgc cgtacggcac ctacacacag accgcggcgt cgaacatcct
     3061 gcgggggttg ccgggtctcc tgctcgatca gcagttcggg atcctgccga acgcgccagc
     3121 ctgcctctgt gcgctggccg ggttctggcc actggcgagg cggcgtccgc gcctggcggt
     3181 cgagctgtcg ctgctggtgg cggcctacct ggccgcggtc tcgtcgtatc acatgtggtg
     3241 gggcggctgg agcgcgccgg cccgcttcgc ggtgcccgtc atgctcgcgc tcgccgtgac
     3301 ggccggcgcc tactgggcgg cggcccggcg cgccggccgg gccgcgggcg ccgcgctgct
     3361 cggcctcacc ctgctgatca caggaacgat gacgtttggg ggcgggcgat cgatcttcaa
     3421 ctcccgcgac gggttcgcgc gatggctcga tcgcgccggc ccgaacgtcg atctcccgcg
     3481 cgcgctgccc agcttcttcc gcgacggccg ggcgctggcg ttcggccagg cggccgcctg
     3541 gctgctggcc ttcgtgctgg ccctcctggt gctgcgcctc gtcgtgcggg cggcggagcg
     3601 gcggccgggg gcgaacgtgc gcctgcgcct cgcgactgcc gtgccggcgc tcgcggcgct
     3661 ggctctcgga ggggcaacaa cggtggggtg gcgccttggc ggcgccaatg gggtggcgcc
     3721 gctcgctggg cagctggcgc tgctgcaggc gtacgacccg gagcggctcc cgatacgcat
     3781 cggggctcct gccaggctcg cgctgaagca acctgaggga cggccggctg cggaaggggt
     3841 gctgctggcg cttgggccgc tgccggctgg cacgtaccgg ctggaaccgg catcgctgcc
     3901 ggcggcgcgc ggaaccacga cggttcgtct cggccgcgac gagcgcccgc tggccacgtg
     3961 ggatctagac ggacgagcgg agagaacgct cgagctgccg gcaggcgcgg cgttcctcgc
     4021 gatcgaggga gacgaggacg cgcgcgcgac cgcgcccggc atcgcactgc ggcccgtgac
     4081 gctgattccg cccggcctca gggcgtcacc aggcttcgcg cgccacgcgg ccgcctatgg
     4141 gccggtcatc gtctacgcgt tcgacggccg ggcctggctc gaggagccgg ggctctgggt
     4201 gcggggcggg gtggaggttc ccctggtctt cgcgaagggc ggcgcgcgcc gtctcgaggt
     4261 gctgctgcgg aacgggccgc tgccgaacaa ggtggaggtt gacggcggga cgtggcggct
     4321 cgacctgcag ctggcgccgc gggaaacgcg cgccatcgag gttcctctgt ggggaacgcc
     4381 ggccgccgcg ctcgtgcggg tgaaggcgga gcgcggcttc aggccgtcgg agacggcgcc
     4441 cggaaacagg gacgagcggt acctcggagt gtggatggaa gtgccgcgcc cctagaacgc
     4501 ccctagaacc gcgccacccc catgaagtag agacccttca acttgaagtc gccgttgtcc
     4561 gatttgacct tgtacatcac gtcgattgag cggtagccgg cctgcacccc gaagttgttg
     4621 gtcaggttca gcgtgccgta gatgtcgaag tcgaggtact tcccgtcgta ccggtctttc
     4681 tcgtcggaca cgcttgccgg cagcttgaac ccggtgaact ccgcggtgag cgagacgttg
     4741 ccgagcgggt agatccggcc gatgaggccc agcgccggaa tgggcgcccg cgcccgcgtg
     4801 tactccgggt cgggaagcac cgggctctcc aggttcacct cgacgtcggt gtatttcgcg
     4861 tcgaccacga acccgaggaa cccgcggtcg cggtagagga agtcgtactc gtaggagaag
     4921 cggtacgcct tccagtcgag cgacgagttc accgggacgc cgacgttgta cttgatcccg
     4981 ttgaagacaa tcgcgcgctc gagccggggc tgctcggcct cgaagagaat cgggatgtac
     5041 tggaagcgca gcttgtgctt cttcccgggg cggagcacca cgcggaactc cttgaagcgc
     5101 gtcttctcca tgcccaggtc gttgacgccg tcgatctccg tccccacgat ccccagcgac
     5161 tcgctcgaca ccaggatcgt cggcgtcggg ttccagaggc cgaaggaggc ctcgacatgg
     5221 taccgctcgc ccgtggcggg ctggctcgtc gggtactgct tgtactgggc gctggcggtc
     5281 gaggcgccca ccgcgagggc cgcggtcagg cacgctgcga ggcggatcgt gtttctcatg
     5341 ctcatcccca agcaggccgc cggcggcctg ttcgaatccc gtgctacggc ccggcgcacg
     5401 gtcagctgaa gtgagcgccg ggattaggca gttccctgca tcgtgttatc ggcgggagcg
     5461 gcgcttcctg caggggccat ccaccggcgc accgaggcca ccacgatcac caccacgcag
     5521 gccatcatga cgaccgtcat gatgctgttg aggtagccct gcacgtgcag cgcagggtcg
     5581 gggccgaccg ccatcggcca gaagttgtcg cgtacgctca ggaacccggc cgagagggta
     5641 gtggtcgcca ggaagaccag cgggacgacg gtcacccagg cgtagcgggc acggccgagg
     5701 ttgaccagga tcgtcgtccc gatgagcagc gcaatgaccg cgagcagctg gttgctgatc
     5761 ccgaacatgg gccagatcgt gccgatgctc cccgtccaga tgaagtaggc ccaggccacc
     5821 accaccgcgc cggtcgagat caccgtgccg ggcatccagt cggtgcgggc aaacggcggg
     5881 tagaaccggc cgaagaactc ctgcacgagg aagcgcgcca ccctggttcc ggcgtcgatc
     5941 gttgtcagga tgaacagggc ctcgaacatg atggcgaagt ggtaccagta gtccatcagg
     6001 cccttcatcc cgggcagcgc gctgaagatc tgcgccatgc ccaccgccag cgacacggcg
     6061 ccgccggttc tcccggccac cgtctcgccg accgccgcct cgagcgcctc gaggttgacc
     6121 ggcgggatgc cgagagtggc gaaggccgcc gcgggcacgt tgatggcgaa gtagtcgccg
     6181 gggtggagtg ccgtggcggc gatgagcgcg acgatcccga ccagcccctc gatcagcatc
     6241 gcgccgtagc cgatcggccg gatgtcagac tccttgtcga tcatcttcgg ggtcgtgccc
     6301 gacccgacca ggccgtggaa gcccgagatc gccccgcagg caatcgtgat gaagacgaag
     6361 gggaagagcg cgcccttgat gatcggcccg ccgccggcgg cgaagctcgt gacggccggc
     6421 atgtgcagct cggggttcac gatgatcacg ccggcgacca gcaggaggat ggtgccgatc
     6481 ttcatgaacg cgctgaggta atcgcgcggc gcgagcagca tccacaccgg cagcaccgag
     6541 gccaggaacc cgtaggccgc catcgcgatc accagctgct ggtgcgtgag cgtgaagtac
     6601 tggccgaacg ccgtcgcggc cagcggcttc ccgaagacga cggccgccag caggccgatc
     6661 accccgatga cggtcgcctc ggcgatcttc ccgcggcgga accggtacat gtagaagccc
     6721 atgaacaggg ccagcgggat cgtcgcgccg atcgtgaagg tgccccacgg gctctggctg
     6781 agagcgttga cgaccacgag gccgaggccg gccagggcga tgacgacgat gaagaggatg
     6841 gcgaggccgg tggtcagccc ggccaccggg ccaatctcga tccgggcgat ctcggcgagc
     6901 gagcggccgc cgcggcgggt cgaggcccac aggatgatga ggtcgtgcac ggcgccggca
     6961 agcacgacgc cggcaaccag ccacagcagg cccggggcgt agccgaactg cgcggccagc
     7021 acggggccga ccagcgggcc ggcgccggtg atggcggcga agtggtggcc gaagagcacc
     7081 cagcgcgagg tggggtagta gttgtgaccg tcgtactggg tgtgggccgg cgtgcggcgc
     7141 gtgtcgtcca gcaccaggag gcgcgcggtg atgaaggcgc tgtagtagcg gtaggcgatg
     7201 acgaggatgc acaggatcgg aaccaggagg tagagcgcgt gcatcggcag atgatacttg
     7261 ataccttgac aggatgcgtc ccgcccggcg gctgctgccg tacctgctcg cctacaggcg
     7321 ccccctggcg ctggggctcg ccagcgtcgt cgccaccacg gccgtttcgc tggtctcgcc
     7381 ctgggtgctc aagtacgcca tcgacgacct gacggccggg gtgacgcgtg ggaagctcgc
     7441 ctcgtacgcg gcgctgctgc tgggtctcgg cgttctcagc ggcgccttcc gcttcctgat
     7501 gaggcgcctc atcgtcggcg tctcacgaca catcgagtac gacctgcgga accagttctt
     7561 cgccaccctg cagcggcagc cggccgctta cttccagtcg aggcggacgg gcgacctgat
     7621 gtcgcgcgcc acgagcgacc tgaacgccgt gcggacagtg gtcggccccg ccatcatgta
     7681 cacgaccaac accgcgatcc tcttcgtggt ggcgatcgcc ctgatggcgt ccatcgactg
     7741 gcgcctgacg ctgatggcgc tggtgccgct gccggccgtc tcggtggccg tccgcttctt
     7801 cggccaggcc atccaccacc gcttcgagcg catccaggcg cagctctccg acctgagcgc
     7861 ggtggtgcag gagacgctcg ccggcgtccg cgtcgtccgg gcctaccggc aggaacaggc
     7921 cgagatcgag cgcttcgccc gctcgaaccg ggagtacctc gagcggaacc ggatcctgat
     7981 ccgcctccag agcctgttct tccccagcct gtcgctgctg ctcggcctcg gcacgctgct
     8041 catcctctgg ctcggcgggc gcgcggcggt ggcggggcgg atcaccatcg gcgagttcgt
     8101 cgccttcaat gcctatctgg cgatgctggc gtggccgatg atcgccttcg gctgggtcac
     8161 caacatggtg cagcgggggt tggcctcgtg ggagcgcctg ctggaggtgc tcgacgccga
     8221 gccgtccatc gccgaaccgc cacccggccg gctggtgcgc ctgccggaga tccgcggcga
     8281 gatcgagttc agggagctga gtttcgcatt cggcgagcag gtggtgctgc gcgacgtctc
     8341 ggtccgcatc gaggcgggtc agacgctcgc gctcgtcggg gccaccgggt cgggcaagtc
     8401 ggcgctcatc agcctgctgc cgaggctgta cgacccgccg gcgggtacgg tcttcctcga
     8461 cggggtggac gtgcgcaacc tttcgctcgc ccagttgcgg gccgcaatcg gcttcgtctc
     8521 gcaggagccg ttcctcttct ccgacacgct cgccaacaac atcgccttcg gcagcgagcg
     8581 gccagcggcc gagcagatgg accgggtcga gtgggcggcc gggatcgctc agctcgacaa
     8641 ggacgtcgcc ggctttccga agggctacaa cacgatggtg ggggagcgtg ggatcacgct
     8701 gtcgggcggg cagaagcagc gcacggcgct ggcgcgcgcg ctggtggccg acccgaaggt
     8761 gctgatcctg gacgactccc tgtcggcggt ggacaccgac acggaggagc ggatcctgtc
     8821 ggggctgcgc ggcgtgatgc gcgagcgcac ctccatcatc gtctcgcacc gggtgtccac
     8881 ggtgcgcgag gccgacctga tcctggtgct cgagggcggg cgcgtggtgg agcggggcac
     8941 gcacgacgag ctcgtgcgcc tcgacgggtt ctacgccggg ctctaccgga agcagctgct
     9001 ggaggaggag ctggcggcgt cttgattccc gaatcctgaa tcctgaatcc tgatccccga
     9061 tccctgatcc ccgatccccg atggcgcacg aagaagaggt tctcggcaag gcgtacgacg
     9121 cgcggctgat gcggcggctg ctggggtaca tgcgccccta cgcaggccac gtggccgtgg
     9181 ccttcgccgc gatcgtcggc gcatcgctgc tgcagctggc gcagccctac ctcgtgaagg
     9241 tggcgatcga ccggtacatc gcccagggaa atgccgcggg cctcgacctg attgcggggg
     9301 ccttcctggc gatcctgctg gcggccttcg gcctcgagtt cctccagacc tacgtcctgc
     9361 agctggtcgg ccagcgcatc atgtacgacc ttcgggtgca ggtgcaccgg cacctgcagg
     9421 cgctcgacct gtcgttctac gaccggaacc cggtcgggcg gctgatgacg agggtgacga
     9481 ccgacgtcga cgtgctcaac gacctgttcg cctcgggcgt catcacggtc ttcggggacg
     9541 tggtgacgct gaccggcatc atggtcgtgc tgctggtgat ggactggagg ctggcgctgg
     9601 cggccttctc ggtgctgccg ctgattgtcc tggtcaccca gtggttccgg cgccacgtcc
     9661 gcgagtcgta ccggacggtg cgtgtgctga tcgcccggat caacgccttc ctgcaggaga
     9721 acctgggcgg gatggccacg gtgcagctgt accgccgcga ggccgcgcag ttccggaagt
     9781 tcgacgagat cgacggcgca caccgcgacg cgaacctcga ctccatcttc tactacgccg
     9841 tgttctaccc ggcgatcgag gtgatcggcg cgctcgcggt ggcgctgatc atctggtacg
     9901 gcggcgggct cgtgctggcc ggggcgctga cgctggggtc gctcgtcgcc ttcatccagt
     9961 actcgcagcg gttcttccgg ccgatcagcg acatctccga gaagttcaac ctcctgcagg
    10021 cggcgatggc ctcgtcggag cggatcttca gactgctcga cacccccccc gcgatcgttt
    10081 cgcccgcgcg ccccgcggcg cgggccgtga cccggggaaa gatcgagttc cgggacgtgg
    10141 gcttcgccta tcacccgggg gagccggtgc tccggggggt ctccttcgtc gtcgagccgg
    10201 gcgaacagg
//
LOCUS       DAOPQX010000101        10173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7515_length_10173_cov_4.608421, whole genome shotgun sequence.
ACCESSION   DAOPQX010000101 DAOPQX010000000
VERSION     DAOPQX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10173
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7515_length_10173_cov_4.608421"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKK95_07315"
     CDS             <1..199
                     /locus_tag="PKK95_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18059.1"
                     /translation="ELEAWLEGWGRGLSEMNYVRTGARTENMLDVHIVTDTDVARQDG
                     AAAKINAVRDPARPLRVGTAS"
     gene            complement(278..1789)
                     /locus_tag="PKK95_07320"
     CDS             complement(278..1789)
                     /locus_tag="PKK95_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOC18060.1"
                     /translation="MSHRLRSGLVLGLAVAGLSLTLAARERPTSSIAAAQLGVADVLF
                     SQAEYRAAMRVYLQASLVEDAELRERAREGAIRGALRIGEFGVAATHAAALRSARPAD
                     PTALTLSGDALWASGLFDEAEAAYRDVQEIEPGHGPSLAGLAKALASRSLLDEAQRAA
                     EASIETAASTEGWHALGQILERRRDYRGAAEAYTRFVESLSPRERAGRAHWTMGQVHF
                     LRSFGNRTPYQHVSEPGQVHAVPFRLVDDKVIVQAKANGSRLDFAVDTGSEQAVLSVR
                     VARRLGVKPVSYTISAGVGQFGLRGLQLGRLDSFEVGTLKLRNVPVLIKSPVLPDTPT
                     DDVESFSPLAVGLSAVIDYGTRKLVMSESLPEEVPDFELPLRMHRLATVRGEVNGRPR
                     SFIVDTGGEVISLNTTTARGMFRPIERHRIRLRVHGASGWDPDAYLLPGVNLDFSEIV
                     YRNTPVVVLNLKMPSALLGYDIGGIVGHKFLGKYRVALDLPRSTVRLHKVLRP"
     gene            complement(1871..2332)
                     /locus_tag="PKK95_07325"
     CDS             complement(1871..2332)
                     /locus_tag="PKK95_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC18061.1"
                     /translation="MRNLLRKLLLVDLLKGLWLTFTYTPQPAFTFQYPKERRPTAPRF
                     RGVLRLQVEPGTGAQTCIVCDQCAKACPDDLIELGGHREPGQKLKALDYFDFNLSRCS
                     FCGLCAEVCPTKPIKALVMSEDYELATYGRDRQVLRIDEMYDGLPIQAYTR"
     gene            complement(2339..2869)
                     /locus_tag="PKK95_07330"
     CDS             complement(2339..2869)
                     /locus_tag="PKK95_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003874821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOC18062.1"
                     /translation="MGLIDHRFDDNVITTNLDAVLNWARKSSLWPMGFGLACCAIEMM
                     AAGDPRFDIARFGSEVFRASPRQADLMIVAGTVTKKMAPVLRRLYDQMPEPKWVISMG
                     SCSNAGGPFPTYSVLQGVDKVVPVDVYVSGCPPRPEALLYGLMRLQDKIMKEGTVLRR
                     ERVIKTGSTEPVLVEG"
     gene            complement(2983..3243)
                     /locus_tag="PKK95_07335"
     CDS             complement(2983..3243)
                     /locus_tag="PKK95_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOC18063.1"
                     /translation="MAYVICEPCIGTKDTACVDVCPVDCIHPRKDEGDFATAELLYIH
                     PDECIDCGACVPACPVEAIFALDEVPEKWQHFIQINAEHYQK"
     gene            complement(3263..4030)
                     /locus_tag="PKK95_07340"
     CDS             complement(3263..4030)
                     /locus_tag="PKK95_07340"
                     /inference="COORDINATES: protein motif:HMM:NF020897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC18064.1"
                     /translation="MPHRPGTGGEGAPGLTSIDKAFDVCEALSHAPEGLSLSELARRL
                     KLPRPTVHRLLAVLRRRGYVRQEEDTQRYSLGTRLLDLGFRALGRSELRLHAYAILRE
                     YAAQSGQRVFLAVPGSGEVTYIWSPAPGGETAMRTVYGHEMPAHCSLYFFDKAQTGRR
                     LTCLKLQDARDVGSSEALARRFGHEEPPGGGAQRLYCTCAPVRDYTGREVARVGIFGH
                     GPDDRPILGVHNRGAWELARHLSMRLGYLSAVALGVA"
     gene            4086..4631
                     /locus_tag="PKK95_07345"
     CDS             4086..4631
                     /locus_tag="PKK95_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18065.1"
                     /translation="MSTRTPAPWTPYAALIAALALLATLPASPAAAPGQLGLEAREVP
                     GRPSDAHRRLFTPRAAPPGAYIVTVLASPMKGAREEVMSALGVAEPGHPPSPPADAPW
                     GVRRLEVAEAFGASGPYEPIRVARLFNGRRVEVSRGPVMRHGRPIASVTLISPYPDAT
                     LSRIEPGTLVIVLRLGPAAGM"
     gene            4743..5975
                     /locus_tag="PKK95_07350"
     CDS             4743..5975
                     /locus_tag="PKK95_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18066.1"
                     /translation="MPPLLALAAALLCGFAGLARPAAAQGLPQAPITWLGGRGFLSGS
                     VSAAAASGGTDTSFNSGDYGQDTMRLVELTLAPSIALHDAAFFSADLRAAGSVNRSDW
                     YFRPARLVVGVRPFPGRPLTVSAGLLQTPFGQSGRGYGRENLLVGLPLIYQYRTALQA
                     DGSRGAGVPGGEGPYPYPEYHGYGPPAAAAQSYSYSYVAPGLPLVDTRGWNAGVRLDA
                     GGDRAQVAAALVRGSLSNPLSRDDRPGWQLAGRAAGQPIVGLVVGVSGARGRYARVPG
                     SGASLAETALGADAEISRGYWLVRGEVVHSRREVAPPGAPSPTETLGVTGLDVEGRYR
                     IAPGFYLAGRVGRLWFGGARGTALSWDADVTRFEGGLGWSLSRPLLVKASYQYNRRPG
                     GIGREQLHRVAMQALVWF"
     gene            5975..6628
                     /locus_tag="PKK95_07355"
     CDS             5975..6628
                     /locus_tag="PKK95_07355"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOC18067.1"
                     /translation="MRAILLPAAIVLAALAATSALPAVDGQAVPGWLQGEVAIRSPLV
                     APPVEGQPHARPREHRRAVVFFEAPPHRNDGLVSGRAEMRQRDATFDPHLMAITAGTT
                     VAFPNDDRTYHNVFSLSKPRRFDLGRYAAGRSKDVRFERPGLVRVFCDIHAHMNAFIH
                     VFDHRFYAATNERGRYRIDRVPPGTYRVTAWYEGVDQQTREVVVHAGAAAELDFVLH"
     gene            6628..8472
                     /locus_tag="PKK95_07360"
     CDS             6628..8472
                     /locus_tag="PKK95_07360"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC18068.1"
                     /translation="MSTRTMLGSLTNRVFLASALLAVFSTAAATLVVSRMVTREAEAG
                     LRRGLDEAAALVTEWQSTLSGNFQLAARLIAEAPPLKAAIDTGDPATVAPLARDYHHR
                     IRSAVFIVTDREGRVLAREGASAGAGGLDQLPAGEAAAGREAVSYWPHPDGVLQLITV
                     PVLAPPQVIGTLSVGFLFDRARAEQVRRLTDSEVAFAVGGVVRATTLPHAAPLEALPG
                     AGHDQALSRLDLDGEEFVALVRPLVSPAPAGSVPGSPPPRAATASRQAGAAAIVLQSR
                     TERLRFLAPLRAALGVTALLAVLGAVVLSFLVSRTITRPLDAITTRMREVAATGDLTR
                     KIALPSGRWGDEDARLLAGTFNALTDSIARFQREAAQRERLSSLGRMATVVAHEIRNP
                     LMIIKAALHPLRRAPASAEHVAEAARDIEEEVARLNRIVNEVLDFARPIRFEMGEADL
                     GAVCQQAASAVAAADAGPPVALRLPGDPVTIVTDAERLRAVLVNLLTNARHATRAAAA
                     AAGPAATGAGGDLPAVELALGLEDGRAWVEVRDRGPGMSAEQLARAFEPFFTTTPGGT
                     GLGLAISRNVVDGLGGSIAIDSVEGRGTTVRVELPVPGRARDRQRGDA"
     gene            8469..9836
                     /locus_tag="PKK95_07365"
     CDS             8469..9836
                     /locus_tag="PKK95_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC18069.1"
                     /translation="MTADARGSILLVDDEEKILKALARALRQEGHEVVEATRSPDARR
                     LLRERPFDVLVTDYVMPDLSGLELIRTLTETTPEPERPQILLMTAHATVESAIEAMKL
                     GALDYLQKPFEVDELLVTVARAVELQRLRGQNRYLMSERDEEFNHYGIVGRSGAIQKV
                     IRTLELVAESKSTVLITGETGTGKEMVARAIHSRSAQRRMPLIKVNCAAIPESLLESE
                     LFGHVRGAFTGATATKRGKFALADGGTIFLDEIGTMSAALQAKLLRVLQDREFEPLGA
                     ERTQRVDVRVIAATNRDLRRMVEDGAFLEDLYYRLLVVPIEMPPLRDRREDVPLLVEH
                     FLRKHQKQAGKRIDGLAPGVLPALVDYPWPGNVRELENTIERAVVLSTGSVITAAEIA
                     VIGSPKPRSSLPSLRLHENVEWTERETLRMALEMARGVKKDAAELMGISQRALSYYLA
                     KYPGD"
     gene            9979..>10173
                     /locus_tag="PKK95_07370"
     CDS             9979..>10173
                     /locus_tag="PKK95_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18070.1"
                     /translation="MRQLLLAVAAAAAVASWPQGPADPPAVREIEVVAEKFKFTPERI
                     EVTQGDRVRVTVRSADVSHGW"
BASE COUNT         1418 a         3720 c         3556 g         1479 t
ORIGIN      
        1 cgagctcgag gcctggctgg aaggctgggg ccgcgggttg agcgagatga actacgtgcg
       61 cacgggcgcc cgcaccgaga acatgctcga cgtccacatc gtcaccgaca ccgacgtcgc
      121 gcgacaggac ggggccgccg cgaagatcaa cgcggtgcgc gaccccgcgc gcccgctacg
      181 cgtcggcacc gcgagctgac cacgaaggcc agacgagacc acgaaggacc aagaccacaa
      241 agggatcaac cacaaagggc actccggtcc ttcgtggtta cggtcgcagc accttgtgca
      301 gtcgcacggt gcttcgcggc aggtcgagcg ccacccggta cttccccagg aacttgtgcc
      361 ccacgatgcc gccgatgtcg tagccgagca gggcgctcgg catcttcagg ttcaggacca
      421 ccaccggcgt gttgcggtac acgatctcgc tgaaatccag gttcaccccg ggcagcaggt
      481 acgcgtcggg gtcccacccc gacgcgccgt gcacccgcag gcggatccgg tggcgttcga
      541 tcggccggaa catcccgcgc gccgtcgtcg tgttgagcga gatcacctct ccgcccgtgt
      601 ccacgatgaa gctccgcggc cggccgttca cctcgccgcg caccgtcgcc aggcgatgca
      661 tccgcagcgg cagctcgaag tcggggacct cctcggggag cgactcgctc atcaccagct
      721 tgcgggtgcc gtagtcgatc accgccgaca ggccgaccgc cagcggcgag aagctctcca
      781 cgtcgtcggt cggggtgtcg ggcagcaccg ggctcttgat cagcaccggg acgttgcgca
      841 gcttcaacgt tcccacctcg aagctgtcga gccgcccgag ctggaggccc cgcaggccga
      901 actgccccac cccggcgctg atcgtgtagc tgacgggctt cacgcccagc cggcgcgcga
      961 ccctcaccga cagcaccgcc tgctccgagc ccgtgtcgac ggcgaagtcg aggcgcgagc
     1021 cgttcgcttt cgcctggaca atgaccttgt cgtccaccag ccggaacggc accgcgtgca
     1081 cctggcccgg ctccgatacg tgctggtagg gcgtccggtt cccgaacgac cgcaggaagt
     1141 gcacctggcc catcgtccag tgcgcgcggc ccgcccgttc gcgcggcgac agcgactcca
     1201 cgaagcgcgt gtacgcctcg gccgcccccc ggtagtcgcg ccggcgctcg aggatctgcc
     1261 cgagggcgtg ccagccctcc gtggacgcag cggtctcgat tgacgcctcg gccgcccgct
     1321 gcgcctcgtc gaggaggctg cgcgaagcca gcgccttcgc aaggccggcg agcgacggcc
     1381 cgtggcccgg ctcaatctcc tgcacgtcac ggtaggccgc ctcggcctcg tcgaacagcc
     1441 ccgacgccca gagggcatcg cccgacagcg tcagcgccgt cgggtcggct ggcctcgccg
     1501 aacgcagggc cgccgcgtgc gtggccgcga cgccgaactc gccaatccgc agcgcgcccc
     1561 ggatcgctcc ctcccgggcg cgctcgcgaa gctcggcgtc ctccacgagc gaggcctgca
     1621 ggtacactcg catcgcggcg cggtactcgg cctggctgaa caggacgtcg gccacgccca
     1681 gttgcgcggc cgcgatggaa gaggtagggc gttcccgcgc cgccagcgtc agcgagaggc
     1741 cggcgacggc gagcccgagg acgaggccgg aacgaagtcg atgggacatg tgccgctccg
     1801 tgctgccggg cgtcggagac gcaaccgggg gggccatcct gacgggagtc cagtccctgc
     1861 ccgtgcctcc ctaccgggtg tacgcctgga tggggaggcc gtcgtacatc tcgtcgatgc
     1921 gaagaacctg cctgtcgcgg ccgtaggtcg ccaactcgta atcctcgctc atcaccagcg
     1981 ccttgatcgg cttggtcggg cacacctccg cgcacagccc gcaaaagctg caccgcgaga
     2041 ggttgaagtc gaagtagtcg agcgccttca gcttctgccc cggctcgcgg tggccgccga
     2101 gctcgatcag gtcgtccggg cacgccttgg cgcactggtc gcagacgatg caggtctgcg
     2161 cgccggtgcc tggctcgacc tgcaggcgca ggacgccccg gaagcgcggc gccgtcggcc
     2221 ggcgctcctt ggggtactgg aaagtgaagg ccggctgggg cgtataggtg aacgtcagcc
     2281 agagcccctt caacagatcg acgagcagga gcttcctcag caggttccgc atgactgact
     2341 acccttccac cagcacgggc tcggtcgagc ctgtcttgat cacgcgctcg cgccgcagca
     2401 cggtcccttc cttcatgatc ttgtcctgca ggcgcatcag cccgtagagc agcgcttcag
     2461 ggcgcggcgg gcacccggac acgtacacgt ccaccggcac gaccttgtcc acgccctgca
     2521 gcacgctgta ggtcgggaac ggcccgcccg cgttcgagca gctgcccatc gagatcaccc
     2581 acttcggctc gggcatctgg tcgtacaggc gccgcaagac gggggccatc ttcttggtca
     2641 ccgtgccggc cacgatcatc aggtcggcct ggcgcggcga ggcccggaac acctcggagc
     2701 cgaaccgcgc gatgtcgaag cgcgggtcgc ccgccgccat catctcgatg gcgcagcagg
     2761 ccaggccgaa ccccatcggc cagagcgacg acttccgcgc ccagttcagc acggcgtcca
     2821 ggttggtggt gatgacgttg tcatcgaacc ggtggtcgat caggcccatg agtgcctcgt
     2881 gacgggattc gggaaccggg gattcgggaa tcgggattcg aaaataggga ttcgggatcg
     2941 cggtctcctg gatcctgaat cccgaatcct gattcctgac tcctacttct ggtagtgctc
     3001 cgcgttgatc tggatgaagt gctgccactt ctcgggcacc tcgtcgagag cgaagatcgc
     3061 ctcgacgggg cacgccggca cgcaggcacc gcagtcgatg cactcgtccg ggtggatgta
     3121 gagcagctcc gcggtggcga agtcgccctc gtccttcctc gggtggatgc agtccaccgg
     3181 gcagacgtcc acgcacgccg tgtccttggt gccgatgcag ggctcgcaaa tcacgtacgc
     3241 cattggttcc tccgtgtgtt tctcacgcca ccccgagcgc gaccgctgag aggtatccca
     3301 ggcgcatcga caggtgccgc gccagctccc acgccccgcg gttgtggacg ccgaggatcg
     3361 gccggtcgtc cggaccgtgg ccgaagatcc cgacccgcgc cacctcccgg ccggtgtagt
     3421 cgcggaccgg cgcgcacgtg cagtagaggc gctgcgcccc gccgcccggc ggctcctcgt
     3481 gcccgaagcg tcgcgccagc gcctcgctgc tgccgacgtc gcgcgcgtcc tgcagcttca
     3541 ggcaggtgag ccgccgcccc gtctgggcct tgtcgaagaa gtacagcgag cagtgcgccg
     3601 gcatctcgtg cccatacacg gttcgcatcg ccgtctcgcc gccgggcgcg gggctccaga
     3661 tgtaggtcac ctcgcccgag ccgggcacgg ccaggaacac ccgctgcccc gactgcgccg
     3721 cgtattccct gaggatcgcg tacgcgtgca gccgcagctc gctgcgcccc agcgcccgga
     3781 accccaggtc gagcagccga gtccccaggc tgtagcgctg cgtgtcctct tcctggcgga
     3841 cgtacccccg gcgccggagc accgccagca ggcgatgcac ggtggggcgc ggcagcttca
     3901 accgccgggc cagctcggat agactcaagc cctcgggcgc gtggctcagg gcctcgcaga
     3961 cgtcgaacgc cttgtcgatg gaggtcagcc cgggggcgcc ctccccgccg gtgccgggtc
     4021 ggtggggcaa ttccattatg tggaatgcga tttcgttctc taaacggtac tgcccgcgca
     4081 agaggatgtc aacaagaacg ccagcgccct ggacacccta cgccgcgctc attgcggccc
     4141 tcgccctcct ggcaaccctg ccggcgtcgc ctgccgccgc ccccgggcag ctgggcctcg
     4201 aggcccggga agtgcccggc cgcccttccg atgctcaccg ccgcctgttc accccgcgcg
     4261 ccgccccgcc cggcgcctac atcgtcacgg tgctcgcctc ccccatgaag ggtgcccgcg
     4321 aagaggtgat gagcgctctt ggggtggccg aaccgggcca cccgccatct ccaccagccg
     4381 acgccccatg gggggtcagg cggctcgagg tggccgaggc gttcggggcg agcgggccct
     4441 acgagccgat ccgggtggcg aggctcttca acgggcggcg cgtggaggtg agccggggcc
     4501 cggtgatgcg ccacggccgt cccatcgcct cggtcacgct catctcgccg taccccgacg
     4561 cgacgctctc gcgcatcgag cccgggacgc tggtgatcgt cctgcggctc gggccggctg
     4621 cggggatgta gcttccgggg accggatctc ggcgtccgct tataatcgaa ccatactcgt
     4681 cattggaagc cggccgggca ctccggcggc gaatgttcgt cgcccggtcg ccaaggagga
     4741 ctgtgccccc gctcctcgcc ctcgccgccg cgttgctctg cggtttcgcc gggctggctc
     4801 gtcctgccgc cgcccagggt ctgccgcagg cgcccatcac ctggctcggc ggccgcgggt
     4861 tcctctcggg cagcgtctcg gccgcggccg cttcgggcgg tacggacacg tcgttcaatt
     4921 ccggcgatta cggccaggac acgatgcggc tggtggagct gacgctggcc ccgtcgatcg
     4981 cccttcacga cgccgccttc ttttccgccg atctgcgggc cgcggggagc gtcaaccgct
     5041 ccgactggta tttcaggccg gcacggctcg tcgtcggcgt gcggccgttc cccgggcgtc
     5101 ccctcacggt gtcggcggga ctgcttcaga cgccgttcgg ccagtcgggg cgcggctacg
     5161 gccgcgagaa cctgctcgtc gggctcccgc tcatctacca gtaccgaacc gcactgcagg
     5221 ccgatggatc ccgtggagcg ggggtgcctg gcggcgaggg tccctatccg tatcccgagt
     5281 accacggtta cggtccgccc gcggcggccg cccagtccta ctcgtactcc tacgtcgcgc
     5341 cgggtctccc gctggtggac actcgggggt ggaatgccgg cgtgcggctg gacgccggcg
     5401 gcgaccgggc gcaggtggca gccgcgctcg tgcgcggcag tctctcgaac cctctcagtc
     5461 gcgacgacag gcccggttgg cagttggcag gccgcgccgc gggccagccg atcgttggac
     5521 tggtggtcgg cgtctccggg gcgcggggac gctacgcgcg cgtgccggga tccggcgcgt
     5581 cgctcgccga gaccgcgctt ggagccgacg cggagatctc ccgcggctac tggctcgtga
     5641 ggggcgaagt cgtccacagc cggcgtgaag tggcgccgcc tggtgcgccc tcgcccacag
     5701 agacgctcgg cgtgaccggc ctcgacgtgg agggacggta ccggatcgcg ccgggcttct
     5761 acctcgccgg ccgcgtgggt cgcctctggt tcggcggcgc gcgcgggacg gccttgtcgt
     5821 gggacgccga cgtcacccgg ttcgaagggg gcctggggtg gtcgctctcg cgcccgctgc
     5881 tcgtcaaggc gtcatatcag tacaaccgcc ggcccggcgg gatcgggcga gagcagcttc
     5941 accgggtcgc gatgcaggcc ctggtctggt tctgatgcgc gccatcctcc tccccgccgc
     6001 gatcgtgctc gccgcgctgg cggcgacgtc ggcccttcca gccgtggacg gccaggccgt
     6061 gcccgggtgg ctgcagggcg aggtcgcaat ccgctcgccg ctggtcgcgc cgccagtgga
     6121 ggggcagccg cacgcgcgcc cccgcgagca tcgccgcgcg gtggtcttct tcgaggcgcc
     6181 gccgcaccgc aacgacgggc tcgtgtccgg gcgtgccgaa atgcgccagc gcgacgccac
     6241 cttcgatccc cacctgatgg cgatcaccgc gggcacgacc gtcgccttcc cgaacgacga
     6301 ccgcacgtac cacaacgtct tctcgctgtc gaagccgcgc cgcttcgacc tcgggcgcta
     6361 cgcggcgggc cgctcgaagg acgtgcggtt cgagcgcccc ggcctcgtgc gcgtcttctg
     6421 cgacatccac gcgcacatga acgcgttcat ccacgttttc gatcaccgct tctacgcggc
     6481 caccaacgag cgcggccgct accgcatcga ccgggtgccg ccgggcacct accgcgtgac
     6541 ggcgtggtac gagggcgtcg accagcagac gcgggaagtc gtcgtccacg cgggcgccgc
     6601 cgccgaactg gacttcgtgc tgcactgatg tccaccagga ccatgctcgg ctcgctgacc
     6661 aaccgggtct tcctggcctc ggcgctgctc gccgtcttct cgacggcggc ggccacgctg
     6721 gtcgtcagcc ggatggtcac gcgcgaggcg gaggccggcc tcaggcgcgg gctcgacgag
     6781 gcggcggcgc tggtcaccga gtggcagtcc accctgtcgg gcaacttcca gctcgccgcg
     6841 cgcctgattg ccgaggctcc gccgctgaag gccgccatcg atacggggga tcccgcaacc
     6901 gtcgcgccgc tggcgcgcga ctaccaccac cgcatccgct ctgcggtctt catcgtcacc
     6961 gatcgggaag gccgggtgct cgccagggaa ggggcgagcg ccggcgcggg cggcctcgac
     7021 cagctgccgg cgggcgaagc agccgcgggc cgcgaggcgg tgagctactg gccgcacccg
     7081 gacggcgtgc tgcagttgat cacggtgccc gtcctggcgc cgccccaggt gatcggcacc
     7141 ctcagcgtgg gcttcctctt cgaccgggct cgggccgaac aggtacgccg gctgaccgac
     7201 agcgaggtag ccttcgcggt ggggggcgtg gtgcgcgcca ccacgctgcc ccacgccgcc
     7261 ccgctcgagg cgctgcccgg cgccgggcac gatcaggcgc tgtcgcggct cgacctcgac
     7321 ggggaggagt tcgtggctct cgtccggccg ctggtatccc ctgcgccggc cggctcggtg
     7381 ccaggcagcc cgccgccccg cgccgcgacg gcgtcgcggc aggcgggggc ggccgccatc
     7441 gtgctgcagt cgcgaaccga gcggctgcgc ttcctggcgc ccctccgggc ggcgctgggg
     7501 gtgacggcac tgctggccgt gctcggggcg gtggtcctca gcttcctggt gtctcgcacg
     7561 atcacgcggc cgctcgacgc catcacgacg cggatgcgcg aggtggcggc caccggcgac
     7621 ctgacgcgca agatcgcgct gccctcggga cgctggggcg acgaggacgc gcggctgctc
     7681 gccggcacgt tcaacgcgct caccgactcg atcgcgcgct tccagcgcga ggcggcgcag
     7741 cgcgagcgcc tgtcgtcgct cggccggatg gcgacggtcg tcgcccacga gatccgcaac
     7801 cccctgatga tcatcaaggc cgcgctgcat ccgctgcggc gcgcacccgc ctcggccgag
     7861 cacgtggccg aagccgctcg cgacatcgag gaggaggtgg cgcgcctcaa ccggatcgtc
     7921 aacgaggtgc tcgacttcgc gcgcccgatc cggttcgaga tgggcgaggc cgacctgggg
     7981 gccgtgtgcc agcaggcggc gtcggccgtc gcggcggccg acgccggccc gccggtggcg
     8041 ttgagactcc cgggggatcc ggtgacgatc gtcaccgatg ccgagcggtt gcgggccgtg
     8101 ctggtgaacc tgctcaccaa cgcccggcac gcgacgcgcg cggcggccgc ggccgccggg
     8161 ccggccgcca ccggcgcggg cggcgacctt ccggccgtcg agctcgcgct cggcctcgag
     8221 gacggccgcg cgtgggtgga ggtgcgcgac cgtggccccg gcatgtcggc cgagcagctg
     8281 gcccgcgcat tcgagccgtt cttcacgacc acccccggcg gcaccgggct ggggctggcc
     8341 atcagccgca acgtcgtgga cgggctcggc ggctcgatcg ccatcgacag cgtcgagggc
     8401 cgagggacga cggtgcgcgt cgagctgccc gtgccgggac gggcacggga tcggcaaaga
     8461 ggtgacgcat gacggcagac gcccgcggtt cgatcctgct cgtggacgac gaggagaaga
     8521 tcctcaaggc gctcgcccgg gcgctccggc aggagggtca cgaggtggtg gaggcgacgc
     8581 gctcgcccga cgcgcggcgc ctgctgcgcg agcggccgtt cgacgtcctc gtcaccgact
     8641 acgtcatgcc cgacctctcg ggcctggagc tgattcgcac gctgaccgag acgacccccg
     8701 agcccgagcg gccccagatc ctgctgatga ccgcccacgc caccgtggag agcgcgatcg
     8761 aggcgatgaa gcttggcgcg ctcgactacc tgcagaagcc gttcgaggtg gacgagctgc
     8821 tggtgaccgt cgcgcgggcc gtcgagctgc agcggcttcg cggccagaac cgctacctga
     8881 tgagcgagcg cgacgaggag ttcaaccact acgggatcgt cgggcgcagc ggcgcgatcc
     8941 agaaggtgat ccggacgctc gaactggtgg ccgagtcgaa gagcacggtg ctcatcaccg
     9001 gcgagacggg gaccggcaag gagatggtcg cccgcgccat ccactcgcgg agcgcgcagc
     9061 ggcggatgcc gctcatcaag gtgaactgcg ccgcgatccc cgagtcgctg ctcgagtcgg
     9121 agctgttcgg ccacgtgcgg ggcgccttca cgggggcgac cgccaccaag cgcggcaagt
     9181 tcgccctggc cgacggcggg acgatcttcc tcgacgagat cgggacgatg agcgcggcgc
     9241 tgcaggcgaa gctgctgcgg gtgctgcagg accgcgagtt cgagccgctc ggcgccgagc
     9301 gcacccagcg cgtggacgtc cgggtgatcg ccgccaccaa tcgcgacctg cgccgcatgg
     9361 tggaagacgg cgccttcctc gaagacctgt actaccggct cctggtggtc ccgatcgaga
     9421 tgccgccgct ccgcgaccgg cgcgaggacg tccccctgct cgtcgagcac ttcctgcgga
     9481 agcaccagaa gcaggccggc aagcggatcg acggcctggc tcccggcgtg ctcccggcgc
     9541 tggtggacta cccgtggccg ggcaacgtcc gcgagctgga gaacacgatc gagcgggccg
     9601 tcgtcctgtc gaccgggagc gtgatcaccg ccgccgagat cgccgtcatc gggagcccca
     9661 agccgcgctc gtcgctcccc tcgctccgcc tgcacgagaa cgtcgaatgg accgagcgcg
     9721 agacgctgcg gatggccctc gagatggcgc gcggcgtcaa gaaggacgcc gcggagctga
     9781 tggggatcag ccagcgtgcc ctgagctact acctggcgaa atacccgggg gattgagttc
     9841 cctcgctcgt aaccctttac gcctgccgta agcggcgcgc gcggcgcgcc gcgcccgctc
     9901 cctccacgtt tcctcgtttt ccgagcgttc cgccggcgcc cgcgccgcgg catcgccctt
     9961 gccccatcga ccggcatcat gcgccagctg ctgctcgccg tcgccgccgc tgcggccgtc
    10021 gcctcgtggc cgcagggccc tgccgacccg cccgcggtgc gcgagatcga ggtcgtcgcc
    10081 gagaagttca agttcacgcc cgagcggatc gaggtgaccc agggagaccg cgtgcgggtg
    10141 accgtccgct cggcggacgt gtcgcacggg tgg
//
LOCUS       DAOPQX010000102        10166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7520_length_10166_cov_4.003363, whole genome shotgun sequence.
ACCESSION   DAOPQX010000102 DAOPQX010000000
VERSION     DAOPQX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7520_length_10166_cov_4.003363"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKK95_07375"
     CDS             complement(<1..858)
                     /locus_tag="PKK95_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18071.1"
                     /translation="MSEPLAAGTPGLVVRGARTHNLKNIDLDLPANALVVITGVSGSG
                     KSSLAFDTIYAEGQRRYVESLSAYARQFLERMEKPDVDEIEGVSPAIAIRQKNSIRNP
                     RSTVGTTTEIHDYLRLLYSRVGRTFCRQCGQEVVRESPQVVAERLAALPTGTRLVIGF
                     DMPVVAGPKGGGANGADREGTEGNGSETADEVSETALPFDGAGNGDAADGEAGGNGAD
                     AVAATIDTLRRRGFGRLLVDGRAAVPFDEVEPARLADREALSVVVDRLKVQPDMMTRL
                     TDSIETAYGE"
     gene            complement(1052..1543)
                     /locus_tag="PKK95_07380"
     CDS             complement(1052..1543)
                     /locus_tag="PKK95_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOC18072.1"
                     /translation="MAGHLQGILRVPRYYRRNLPHFRIDGTPCAYFVTWRLAGQQLPL
                     SADERSIVVDALLHFHQQRYDLLSFVVMDDHVHVVVVMARGYTLERVLHSWKSFTAHQ
                     LVRAGARVAPLWQDESFDRVLRDDAELREKLDYIAGNPWKRWPNLETYEWLWTGSESA
                     LMG"
     assembly_gap    1653..1752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1870..2274)
                     /locus_tag="PKK95_07385"
     CDS             complement(1870..2274)
                     /locus_tag="PKK95_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18073.1"
                     /translation="MFRRSVIAGMLVVAVVLAVPALAQEAPQEKPKEAVGDDPISGTW
                     DGTTLMGTQEMTFFMNLRVDKETVSGEIGNYEGSTPVSGTWVDGKLTLNFSYVNGEPV
                     VMTAKLKDGQLAGDLDMNAGQFLATWTAKKKA"
     gene            complement(2336..3343)
                     /locus_tag="PKK95_07390"
     CDS             complement(2336..3343)
                     /locus_tag="PKK95_07390"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HOC18074.1"
                     /translation="MDLARFPRRRYTQGWTPLERMEKLSLLLGGPDLYVKRDDLLGLA
                     GGGNKTRKLEFLVADALGKGCDTLITVGAVQSNHCRLTLAAAVKEGMKCHLVLEERVA
                     NSYRPGASGNNFLYRLLGAASITVVPAGTDLGAAMQKVAGEVTAAGGRPYVIPGGGSN
                     ALGALGYVACAEEILAQAFDTGLSFDHIVCASGSTGTHAGLLAGLVGNNSRIPITGIN
                     VRRPQAEQEPNVHKLAQEVAELLGIPGGVPRSRVRALGDWVGPGYSLPTPEMVEAVGT
                     VARVEGILLDPVYTGKAMAGLFALVRRGEFRRGERVLFLHTGGAPALYAYQDVLVQED
                     V"
     gene            3440..4438
                     /locus_tag="PKK95_07395"
     CDS             3440..4438
                     /locus_tag="PKK95_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18075.1"
                     /translation="MVKFAQFATLWRIVREVDLEGIRRHAEMPTRVLVVGDTEADADG
                     LAALLIGPHPEASAWVNAVGVEHPAAGAKAASAMIVDGPDLVILVSRGRQVSAAMQEV
                     RRAWIDRRARLVQVALEAGGPHGASQAQGETVSLALDGLAEDGIDHLAKALFAAVEPD
                     RRLSLARQFPPLRPCAFNTLIDETAKANATYSLSTGLAEVVPVLGVPLTIGDIIVLTK
                     NQLLMSYRIAMAAGKPGRASELIGEIIGVLGGGLLFRQIARQLAGMLPVVGVVPKVAV
                     AYGGTWAIGRAVTVWATEGRKLTGERLRQLSREGLARGRQVAGSLQRSDKKQNYWL"
     gene            complement(4543..6147)
                     /locus_tag="PKK95_07400"
     CDS             complement(4543..6147)
                     /locus_tag="PKK95_07400"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOC18076.1"
                     /translation="MPSSHPSFRVRPVLIAVLAVCALVLPGAATTLQQEEAWFCPSCE
                     GCTSVLVGKAASVDGSTMTSHSCDSNTDRTWMNIVPNRKHRPGEMAPVYYDPKRTKGP
                     DDADRIETGEIPQVPETYAYLNAAYPIMNEHQLAIGETTFGGKRELKSDEGIIDCPEL
                     YRLVLERAKTAREAIRIADELTKQYGYNDYGEAFTFADPNEVWFFEILGPGKGRKGAV
                     WAAVRIPDDHVAVSANAPRIRKIDPADKERFMASANVFTLAEELGLWSPESGEPFEFC
                     TVYGSRSSLGSRRREWRALSRIAPSLKLDPNAEHYPLSVKAEQKLGVPQVLEIFRDTY
                     QGTPYDMTRSLVKIGKDGKAAPNPIANPFMSNDYLDAFNITSERTIACKRATYLQITQ
                     SRSWLPNPIGGVVWMGYDNPMTTPHTPFYIGIEQMPASYMVDGREKFRRDCAWWAFRQ
                     VSQLATFRWQEMTRDVEQVWRPIEEKAFAGQAAFEEEALRVYKQDPAKARALVTKYSH
                     DMANGAVEAYWKLAEDLWSKYTNTFE"
     gene            complement(6268..8883)
                     /locus_tag="PKK95_07405"
     CDS             complement(6268..8883)
                     /locus_tag="PKK95_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC18077.1"
                     /translation="MTDQLPPLIDRQHFFGDPETAGAQISPDGRFIAFLRPFKGTRNI
                     WVKRAGEPFDNARPITADTRRPISSYFWSRDGKYVLYVQDQGGDENYNVYAVDPAAPP
                     PTGAQVPQARNLTDAAGVRAEILSVPKTDPDALYVALNDRDKAWHDFYRVRISDGTRT
                     LVAKNTERFVGAVFDLKDALRLVVRSPENGDTELLRVDGEGKFTKIYSCNVFETCSPL
                     RFHKDGRRVYLMTNKGPDVDLIRMVLLDVETGREEFVEADPLGRVDLAAARFSEKTDD
                     LILTAYLDEKLRLSWKDPAYEADYRLLRQKLGEDKQIGFGSSTTEEDLFIVSATSDVE
                     PGETYLFDRRTKDLQLQYRVREALPREALAPMQAIRYRSSDGLEIPAYLTLPKAVEPR
                     NLPLVVVPHGGPWARDDWGYDPFAQFLANRGYAVLMPNFRGSTGYGKKFLDAGNDQWG
                     EKMQDDITFGVRHLVSQGTADPKRVGIMGGSYGGYATLAGVAFTPDLYGAAVSIVGPS
                     NLITLLKSIPPYWEAARKIFHERMGDPTTPEGRAQLERQSPLNSASKIRTPLLVIQGA
                     NDPRVNKNESDQIVAALRDRGFPVEYIVAPDEGHGFARPVNNMASFAAAEKFLAKHLG
                     GRYQESMPPDVAARLKEITVDPKSVTVKEPVAPEAVGVPKPAVPLQPGTYPYKAKIAL
                     GPQSIELDIVAEIREEGDRFVVTETASGAAGNAVDRTVLDKATLTLRRREVTQGPMTM
                     RFDVDGGTLDGELNTDGQAKPLKADLGGPLFGDGAGANHVIATLPLEPGYKTAFRNYD
                     VRTQSPQVVQLQVAGEEQVTVPAGTFETVRLEMSSADGSKTTLWVAKDSHRLVKASSV
                     SMAMNGATITSELQK"
     gene            complement(9068..9469)
                     /locus_tag="PKK95_07410"
     CDS             complement(9068..9469)
                     /locus_tag="PKK95_07410"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOC18078.1"
                     /translation="MTDRVFFYGTLMSGFDRRRRAGIDPLLTWVGRGRIQAALFDLGI
                     YPAAVPDPDGTVWGEVYRTDEIERVLAALDEIEGYRADLPEASLYLRVEVPVTFEGGE
                     SSLVWTYFYNAPLGRAERIRSGDYMEHLGVR"
     gene            complement(9728..>10166)
                     /locus_tag="PKK95_07415"
     CDS             complement(9728..>10166)
                     /locus_tag="PKK95_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18079.1"
                     /translation="KRAIDLQLGGQNVLANEELSRMIRGVQDGNAWAVGRFDVLTRNA
                     QLPAEISDRIPPITWFSATGHVNGGVSGRLAVETRDNEAAENLRQVVNGFMALARLQA
                     GANAKQTEVNAMLNSIQLEGDGKTVAVSFTMPAEALELLKSRK"
BASE COUNT         1477 a         3471 c         3433 g         1685 t
ORIGIN      
        1 ttccccgtac gccgtctcga tcgagtcggt caggcgcgtc atcatgtccg gctgcacctt
       61 cagccggtca accaccaccg agagcgcctc gcggtcggcc aggcgcgccg gctcgacctc
      121 gtcgaaggga accgcggccc gtccgtccac cagcagccgg ccgaagcccc gccggcgcaa
      181 ggtgtcgatg gtggcggcca ccgcgtcggc gccgttcccg ccagcctccc cgtccgcggc
      241 gtcgccgttc ccggccccgt cgaacggcag cgctgtctcg ctcacctcgt cggccgtctc
      301 tgagccgttg ccctcggtcc cctcccggtc ggccccgttc gcgccgccgc ccttcggccc
      361 ggccaccacc ggcatgtcga agccgatgac gaggcgcgtg ccggttggca gggccgcaag
      421 gcgctcggcc accacctgcg ggctctccct gaccacttcc tgcccgcact gacggcagaa
      481 ggtccgcccg acgcgggagt aaagcaggcg caggtagtcg tggatctccg tggtggtgcc
      541 cacggtcgag cgcgggttgc ggatactgtt cttctgccgg atagcgatcg cgggagacac
      601 cccctcgatc tcgtccacgt cgggcttttc catccgttcc aggaactgcc gggcgtaggc
      661 ggagagcgac tcgacgtagc gccgctggcc ttcggcgtag atggtgtcga aggcgagcga
      721 cgacttcccc gacccgctca ccccggtgat gacgaccagg gcgttggcgg gcaggtcgag
      781 gtcgatgttc ttgaggttgt gcgtgcgggc gccccggacg acgaggcccg gggtgccggc
      841 tgcgagaggc tcggacattg aacagggcct acggcgatgg ttccgggcac tgccggaaac
      901 acaccatcgt accgcagccg aaggccccgt tcaaccgcag tagcacaggc gtcccgcctg
      961 tggggggcgc agacccagta gcacaggcgt ctcgcctgtg gggggcgcgg attcagtggc
     1021 acaggcgtct cgcctgtgag aggctcagtg gtcagcccat cagcgcgctt tcacttccgg
     1081 tccagagcca ctcgtacgtc tccaggttgg gccaccgttt ccaggggttt ccggcaatgt
     1141 agtcgagctt ctcccgcagc tcggcgtcgt cgcggagaac gcggtcgaag gactcgtcct
     1201 gccagagcgg cgccactcta gcgcctgccc gaacgagttg gtgtgccgtg aaggacttcc
     1261 aggagtgcag caccctttcg agggtgtagc cccgcgccat cacgacgacc acgtggacgt
     1321 ggtcgtccat gacgacgaag gaaagcagat cgtagcgctg ctgatggaag tgcagaaggg
     1381 cgtcaacgac gatgctgcgt tcgtccgccg acaggggcag ctgctggccg gcgaggcgcc
     1441 acgtgacgaa gtaggcgcac ggagtgccgt caatgcggaa gtgcgggagg ttccgccgat
     1501 agtagcgcgg cacgcggaga atgccctgca ggtgccctgc caccgccgag ccatgcgatc
     1561 ttggcgcgag gctggccgct tgaccgggcg gtcagcagaa atcgcgccgc ggagagtgga
     1621 ggagggtgcg aagagttcca tgtcccacag gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nngagacgcc tgtgccactg cccctcctcc cacaggcgag acgcctgtgc
     1801 cactgcccct cctcccacag gcgagacgcc tgtgccactg cctgtcagtg gcactgcctg
     1861 tcggcctgtt caggccttct tcttcgcggt ccaggttgcc aggaactggc cagcgttcat
     1921 gtcgaggtca cccgccagct ggccgtcctt cagcttggcg gtcatcacca ccggctcgcc
     1981 gttgacgtag ctgaagttga gcgtcagctt cccgtccacc caggtcccgc tgaccggggt
     2041 cgagccttcg tagttgccga tctcgcccga cacggtctcc ttgtccaccc gcaggttcat
     2101 gaagaacgtc atctcctggg tgcccatcag ggtcgtccca tcccaggttc cgctgatggg
     2161 gtcgtcgccg acggcctcct tcggcttctc ctgcggcgcc tcctgcgcca gcgccggcac
     2221 ggccagcacg acagccacca cgagcatccc cgcgatcacc gagcgcctga acatgatgcc
     2281 tccctctcga tgccgaccgt tgtgaacaac cgcgttcgag caggccacga tccgctcaca
     2341 cgtcctcttg caccagcacg tcctggtatg cgtacagcgc cggagcgcct cccgtgtgga
     2401 ggaacagcac gcgctcgcca cgccggaact ccccgcgccg gaccagcgca aagaggccgg
     2461 ccatcgcctt cccggtgtac accgggtcga gcaggatgcc ctcgacccgc gccaccgtcc
     2521 cgacggcctc gaccatctcg ggcgtcggca gcgagtagcc cgggccgacc cagtcgccga
     2581 gcgcgcgcac gcgcgagcgc ggcaccccgc cggggatccc gagcagttcg gccacctcct
     2641 gcgccagctt gtggacgttc ggctcctgct cggcctgggg ccgcctcacg ttgatgccgg
     2701 tgatcgggat gcggctgttg ttccccacga gccccgccag cagcccggcg tgcgtgccgg
     2761 tgctgccgct cgcacagacg atgtggtcga agctcaggcc ggtgtcgaag gcctgggcga
     2821 ggatctcctc agcgcaggcg acgtatccca gggcgccgag tgcgttggag ccgccgccgg
     2881 ggatgacgta gggccggcca ccggccgcgg tcacctcgcc ggccaccttc tgcatggcgg
     2941 ccccgagatc ggtcccggcg ggaaccacgg tgatcgacgc ggcgccgagc aggcggtaca
     3001 ggaagttgtt gccgctggcc ccgggacggt agctgttcgc cacccgttcc tcgagcacca
     3061 ggtggcactt cattccttcc ttcacggcgg cggccagcgt caggcggcag tggttcgact
     3121 gcacggcccc gaccgtgatc agcgtgtcgc accccttccc gagggcgtcg gcgacgagga
     3181 actcgagctt tcgcgtcttg ttgccgccgc cggcgaggcc cagaagatcg tcccgcttga
     3241 cgtacaagtc tggcccgccg agcaggagag acagcttctc catccgctcg agcggcgtcc
     3301 atccctgcgt gtagcggcgc cgggggaatc gtgcaagatc catggtggac cggtcgctcc
     3361 tgatgggggc gtatgatagc acccggagtg gcgtcctgca tttccacgtc gaaacagcgg
     3421 ctggcccggg gagggtgaga tggtgaagtt cgcgcagttc gccacgctgt ggcgcatcgt
     3481 cagggaggtc gacctcgagg gcattcgccg tcacgccgag atgccgacgc gcgtgctggt
     3541 agtcggcgac accgaggccg acgcggacgg cctggctgcg ctcctcatcg gccctcaccc
     3601 ggaggcgtcg gcctgggtca atgccgtcgg cgtcgagcac ccggccgccg gcgccaaggc
     3661 ggcgtctgcc atgatcgtcg acggccccga cctcgtcatc ctggtcagcc gcggccggca
     3721 ggtttccgcg gccatgcagg aggttcgccg cgcgtggatc gaccgccggg cacggctcgt
     3781 ccaggtggcc ctcgaggccg gggggccgca cggcgcgtcg caagcccagg gcgaaacggt
     3841 gagcctggcc ctcgacggcc tcgccgaaga cggcatcgac catctcgcca aggcgctgtt
     3901 cgcggccgtc gaacccgacc gccggctgtc gctggcccgc cagttcccgc cgctgcgtcc
     3961 ctgcgcgttc aacacgctga tcgacgagac cgccaaggcc aacgccacct attccttgag
     4021 tacaggcctg gccgaagtgg tccccgtgct cggcgtcccg ttgaccatcg gcgacatcat
     4081 cgtgctgacg aagaaccagc tgttgatgag ctaccggatc gccatggccg ccggcaagcc
     4141 gggacgagcc tctgagctaa tcggcgagat catcggcgtg ctcggcggcg gcctgctgtt
     4201 ccggcagatc gcgagacagc tcgcggggat gctccccgtc gtcggcgtcg tgccgaaggt
     4261 ggcggtcgcc tacggcggca cctgggccat cgggcgggcc gtgacggtgt gggctaccga
     4321 gggacggaag ctgacgggcg agcgcctcag gcagctgtca cgcgaggggc tcgcccgtgg
     4381 ccgccaggtg gccggcagcc tgcaacggtc ggacaagaag cagaactact ggctgtagga
     4441 cagaaccgga gcttcaccgc agagacgccg agaacgcgga aactctgcgc cctctgcgcc
     4501 tctgcggtga atcgtacgtg acagacgtgg ccgcttggtg cgttattcga acgtgttcgt
     4561 gtacttggac cacaggtcct cggccagctt ccagtaggcc tcgaccgcgc cgttcgccat
     4621 gtcgtgcgag tacttcgtga ccagcgcgcg cgccttggcg gggtcctgct tgtagacgcg
     4681 cagcgcctcc tcctcgaagg ccgcctggcc cgcgaacgcc ttctcctcga tgggccgcca
     4741 cacctgctcg acgtcgcggg tcatctcctg ccagcggaag gtggcgagct ggctcacctg
     4801 ccggaacgcc caccacgcgc agtcgcgcct gaatttctct cggccgtcca ccatgtagga
     4861 agccggcatc tgctcgatgc cgatgtagaa cggcgtgtgg ggggtggtca tcgggttgtc
     4921 gtaccccatc cagacgacgc cgccgatcgg gttcgggagc cagctcctcg actgcgtgat
     4981 ctgcaggtag gtggccctct tgcacgcgat ggtccgctcc gaggtgatgt tgaacgcgtc
     5041 gaggtagtcg ttcgacatga acgggttcgc gatcgggttg ggcgccgcct tgccgtcctt
     5101 cccgatcttc accagcgagc gggtcatgtc gtacggggtg ccctggtagg tgtcgcggaa
     5161 gatctcgagc acctgcggca cgcccagctt ctgttccgcc ttgaccgaca gcgggtaatg
     5221 ctcggcgttg gggtcgagct tcagcgacgg cgcgatccgg ctcagcgccc gccactcgcg
     5281 ccggcggctc cccaggctgc tgcgcgaccc gtagaccgtg cagaactcga acggctcgcc
     5341 gctctcgggg ctccacagac ccagctcctc ggccagggtg aagacgttgg ccgaggccat
     5401 gaagcgctcc ttgtcggccg ggtcgatctt ccggatccgg ggggcgttgg ccgagacggc
     5461 cacgtggtcg tcggggatgc gcacggccgc ccacacggcc cccttccgcc ccttacccgg
     5521 gccgaggatc tcgaagaacc agacctcgtt cggatccgcg aaggtgaacg cctcgccgta
     5581 gtcgttgtac ccgtactgct tcgtcagttc gtccgcgatc cggattgcct cgcgcgccgt
     5641 cttcgcccgc tcgagcacca gccggtacag ctcggggcag tcgatgatcc cctcgtcact
     5701 cttcaactcg cgcttgccgc cgaaggtcgt ttcgccgatc gcgagctggt gctcgttcat
     5761 gatcgggtag gcggcgttga ggtaggcgta ggtctcgggg acctgcggga tctcccccgt
     5821 ctcgatccgg tcggcgtcgt ctggaccctt ggtccgcttg gggtcgtagt agacgggcgc
     5881 catctcgccg gggcggtgct tgcggttggg aacgatgttc atccaggtgc ggtcggtgtt
     5941 gctgtcgcac gagtgcgacg tcatcgtcga gccgtccacc gacgccgcct tcccgacgag
     6001 aacgctggtg cacccctcgc aggacgggca gaaccacgcc tcttcctgct ggagggtcgt
     6061 cgcggcgcct ggcagaacca gggcgcagac cgcgagaacg gcgatcagga ccgggcgaac
     6121 gcggaacgag ggatgagaag aaggcatggc gggacactcc ctggggctgg cgaaggatac
     6181 cagaaacgcg aacgggcgag ggcgcccccc cacaggcgag gacgcctgtg ccactgttcc
     6241 ccacaggcga ggacgcctgt gccactgcta cttctggagt tcgctcgtaa tggtggcgcc
     6301 gttcatggcc atcgacaccg acgacgcctt cacgaggcga tggctgtcct tcgccaccca
     6361 gagcgtggtc ttcgagccgt cggcggagct catctcgagc cgcaccgtct cgaaggttcc
     6421 ggcgggcacc gtcacctgct cttcgcctgc gacctgcagc tgcaccacct gcggcgactg
     6481 cgtccggacg tcgtagttgc ggaacgccgt cttgtagccg ggttcgagcg gcagggtggc
     6541 gatgacgtgg ttcgcccctg ccccatcgcc gaacagcggc cccccgaggt cggccttcag
     6601 cggcttcgcc tgcccgtccg tgttcagctc gccgtcgagc gtgcccccgt cgacatcgaa
     6661 gcgcatggtc atcgggccct gcgtcacctc gcgcctgcgc agcgtcagcg tagccttgtc
     6721 gagcaccgtc cgatcgactg cgttgccggc ggcgccgctg gccgtctcgg tcaccacgaa
     6781 gcggtccccc tcttcccgga tctcggcgac gatgtcgagc tcgatgcttt gtgggccgag
     6841 ggcgatcttc gccttgtagg ggtaggtgcc cggctgcagc ggcaccgcgg gcttcgggac
     6901 gccgacggct tcgggggcca ccggctcctt caccgtgacc gacttcggat cgacggtgat
     6961 ctccttcagc cgggcggcga cgtcgggagg catcgactcc tggtaccgtc cgccgagatg
     7021 ctttgccagg aacttctcgg ccgcggcgaa gctggccatg ttgttgaccg gccgcgcaaa
     7081 cccgtggccc tcgtcgggcg ccacgatgta ctcgaccggg aagccccggt cgcgcagcgc
     7141 ggccacgatc tgatccgact cgttcttgtt caccctgggg tcgttggcgc cctggatgac
     7201 gagcagcggc gtcctgatct tcgacgccga gttcagtggc gactgccgct cgagctgcgc
     7261 gcgcccctcg ggtgtcgtcg ggtcgcccat gcgctcgtgg aagatcttcc gcgccgcctc
     7321 ccagtagggc gggatggatt tcagcagcgt gatcaggttc gacggcccga cgatggagac
     7381 cgcggcgccg tagaggtcgg gggtgaaggc aacgccggca agcgtggcgt acccgccgta
     7441 ggagccgccc atgatgccga cgcgcttcgg atcggcagtc ccctggctca ccaggtgcct
     7501 gacgccgaag gtgatgtcgt cctgcatctt ctcgccccac tggtcgttgc cggcgtcgag
     7561 gaacttcttg ccgtagccgg tggagccccg gaagttgggc atcagcaccg cgtagccgcg
     7621 gttggcgagg aactgcgcga acgggtcgta gccccagtcg tcgcgcgccc acggcccgcc
     7681 gtgcggcacg acgaccagcg ggaggtttcg cggctcgacg gccttgggca gcgtcaggta
     7741 ggcgggaatc tcgaggccgt cagacgagcg gtagcggatt gcctgcatcg gcgccagcgc
     7801 ctcgcgcggc agcgcctcgc ggacccggta ctggagctgc aggtccttgg tgcgccggtc
     7861 gaacaggtac gtctcgcccg gctcgacgtc gctcgtcgcc gagacgatga agaggtcctc
     7921 ctcggtggtg gacgacccga acccgatctg cttgtcctcg ccaagcttct gccgcagcag
     7981 gcggtagtcg gcctcgtacg ccgggtcctt ccaggagagc cggagcttct cgtcgaggta
     8041 cgcggtgaga atcaggtcgt cggtcttctc cgagaaccgc gcggcggcca ggtcgacgcg
     8101 cccgagcggg tcggcctcga cgaactcctc acggccggtt tcgacgtcga gcaggaccat
     8161 gcgtatcagg tcgacgtcgg ggcccttgtt ggtcatgagg tagacgcgcc ggccgtcctt
     8221 gtggaagcgg aggggactgc acgtctcgaa gacgttgcac gagtagatct tcgtgaactt
     8281 cccctcgccg tccacgcgca gcagctcggt gtcgccgttc tcgggcgagc gaaccaccag
     8341 ccgcagcgcg tccttcaggt cgaagacggc gcccacgaag cgctcggtgt tcttcgccac
     8401 cagcgtgcgc gtgccgtccg agatgcgcac gcggtagaag tcgtgccacg ccttgtcgcg
     8461 gtcgttcagg gcgacgtaga gggcgtcggg gtcggtcttc ggcaccgaga ggatctcggc
     8521 acggacgcca gccgcgtcgg tgaggttgcg ggcctgcggc acctgggcgc ccgtgggcgg
     8581 cggtgcggcc gggtcaaccg cgtagacgtt gtagttctcg tcgcccccct ggtcctgcac
     8641 gtagaggacg tacttgccgt cccggctcca gaagtagctg gagatcggcc gcctggtgtc
     8701 ggccgtgatc ggccgcgcat tgtcgaacgg ctcgccggcg cgcttcaccc agatgttgcg
     8761 cgtgcccttg aagggccgca ggaaggcgat gaagcggccg tcgggggaaa tctgcgcccc
     8821 ggcggtttcc gggtcgccga agaagtgctg gcggtcgatg agcggcggca gttggtcggt
     8881 catagagggc ctggaaaccg tggacgcggg ctgcgctgct gccgatgcgg tggcaagaga
     8941 ccagctgacc ccgaggcacg cgaacgctcg ccggaccgtg gtcatcggac gcctccgtgt
     9001 agacaaggga cgtgggccca gcagtcgatt gtactagggg cgaggagcca ggagctaggg
     9061 gatgggctca gcggacgccg aggtgctcca tgtaatcgcc cgagcgaatc cgctcggcgc
     9121 gcccgagcgg cgcgttgtag aagtaggtcc agaccaggct gctctcgccc ccctcgaagg
     9181 tgaccggcac ctcgactcgc aggtacaggc tcgcctccgg caggtcggcc cggtaccctt
     9241 ctatctcgtc gagcgccgcg agcacccgct cgatctcgtc ggtgcggtac acctcccccc
     9301 acaccgtccc gtccgggtcg ggaaccgccg cggggtagat gcccaggtcg aaaagcgccg
     9361 cctggatccg gccgcgtccc acccacgtga gcagcggatc gatgccggcc cggcggcggc
     9421 ggtcgaagcc cgacatcaag gtcccgtaga agaacacccg gtcagtcatc gtcaccatcc
     9481 gggaacggcc cggtgtccag aaaacaggca tgaaacggga gcagcgagtg cctggctcga
     9541 ttgggggacg cgtcaggctt tgagtatgcg agcagtctag gagaggccga agctggagtc
     9601 aagccgtcgg caagacgccg gaggccggcg ccggcgggcc cgtacgtgcg acgggcgcgg
     9661 cgttgcaccg cgcccgttcg atgttccgct gttccgcagg gctgaagccc tgcgctacgg
     9721 ccctgcgcta cttccggctc ttgagcagct cgagcgcctc ggccggcatc gtgaacgaga
     9781 cggccaccgt cttcccgtct ccctcgagct ggatcgagtt gagcatcgcg ttcacttcgg
     9841 tctgcttcgc gttcgctccc gcctgcagcc gcgccagcgc catgaagccg ttcacgacct
     9901 gccgcaggtt ctcggccgcc tcgttgtcgc gcgtctcgac cgccaggcgc cccgacaccc
     9961 cgccattgac gtgcccggtc gccgagaacc aggtgatcgg cgggatccgg tcggagattt
    10021 ccgcgggcag ctgcgcgttg cgggtcagca cgtcgaaccg ccccaccgcc cacgcgttgc
    10081 cgtcctggac accgcggatc atcctcgaca gctcctcgtt ggccagcacg ttctggccgc
    10141 cgagctggag gtcgatcgcc cgcttc
//
LOCUS       DAOPQX010000103        10130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7553_length_10130_cov_4.088238, whole genome shotgun sequence.
ACCESSION   DAOPQX010000103 DAOPQX010000000
VERSION     DAOPQX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10130
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7553_length_10130_cov_4.088238"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1519)
                     /gene="accC"
                     /locus_tag="PKK95_07420"
     CDS             complement(146..1519)
                     /gene="accC"
                     /locus_tag="PKK95_07420"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOC18080.1"
                     /translation="MFKKVLIANRGEIALRVIHACREMGIKTVAVYSEADEDSLHVRF
                     ADEDVCIGPPRPGQSYLNVPAVISAAELTGADAIHPGYGFLSESPYLAEVCEACHIKF
                     IGPDPRVIRLMGDKARARRAMRKAGVPVLPGSEGVVESDEEAARIAREIGFPVLVKAT
                     AGGGGRGMRVAAHPGELVQALRTAQREAEAAFGVKDVYLEKYVEAPRHVEFQVLGDHH
                     GNVVHLGERECSVQRRHQKLIEESPSPALTDKVRRKMGHLVADAARAVQYTNAGTFEF
                     LLDGDGRFYFMEANTRLQVEHGVTELVTGVDIVKEQIRVAAGERLSFKQSEVTFTGHA
                     LECRINAECPETFAPSPGTIRVFALPGGPGVRIDTAAHSECTVSPYYDSLLAKIMTYG
                     RDRQEAIARMRRTLEMTVIEGIKTNVALHLKVLAEPDFQAGRLNTAFMNRFVRSASGP
                     RTLAQAG"
     gene            complement(1530..1988)
                     /gene="accB"
                     /locus_tag="PKK95_07425"
     CDS             complement(1530..1988)
                     /gene="accB"
                     /locus_tag="PKK95_07425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOC18081.1"
                     /translation="MIKLEELRQILEMAREHDLAELELEADGRRLRIRKQPAASSAAP
                     FALPPAPAPVPVAPPAPAPAGEDLEADSELAVVKSPIVGTFYRAPAPNAPPFVQVGDP
                     VRKGQTLCIVEAMKMMNNIDSEYEGTLVKIYVENGQPVQYGERLFAIRAK"
     gene            complement(2074..2835)
                     /locus_tag="PKK95_07430"
     CDS             complement(2074..2835)
                     /locus_tag="PKK95_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC18082.1"
                     /translation="MADSARIEELRRRVQKDPASIAFAQLAEEYRRAGRLQEAVEICE
                     TGLARHPGYLSARVTLGRALLEVGELDAAQAELKQVLRTAPENLAALRGLADIHHRRG
                     ELHEALAHYRTALEFAPHDPELERLVEEIQGQLEPATRTPAVVDGLSFEDVQRELLSI
                     AGEAPASPGPDVEAAPDPGVPVESLQEEPHATDPDPAPTVLAASEPAAAALPSSEPAA
                     APPAGPATEALEEWLEAIIADRERRAAAPEGPDGH"
     gene            complement(2859..3599)
                     /locus_tag="PKK95_07435"
     CDS             complement(2859..3599)
                     /locus_tag="PKK95_07435"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOC18083.1"
                     /translation="MLRALRACTLVVAVALSVAACTVKNTDTPPLAGPSELGLSLALQ
                     AVPDVVIQDGESRAQIVVTARDPNSQPVRNLALRVEISFQSRLVDYGTLSPGHNIVTG
                     SDGKAVVTYTPPKAQANSLDSDVVTILVTPLGTDYSTAVSRQVQVRLVPPGVIKGASG
                     AFFTLAPEAPKVGDLLSFDGSSSSTKTGRTIDVYEWSFGDGHVHRHRDSSQTTHVYEK
                     SGSFTVKLSVTDSAGSVSSATRTVYVTE"
     gene            complement(3696..4340)
                     /locus_tag="PKK95_07440"
     CDS             complement(3696..4340)
                     /locus_tag="PKK95_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18084.1"
                     /translation="MTCWGYRKAAGAGLVLFVALASASCGDVVRQGRSPAYLVIDNLM
                     GASGAEPDDFDHTLDSDVVTNIKVTEGENTYFAETFYEDRGELEAHIVLKDPGTPGSP
                     TQPSEANQITINRYHVRFVRADGRNTPGVDVPYEFDGAATATFGPSGARLSFVLVRAQ
                     AKREAPLLALRRMGGAQLISTIAEVTFYGQDQAGNAVKATGQIGVNFADWADPE"
     gene            complement(4351..6591)
                     /gene="pilQ"
                     /locus_tag="PKK95_07445"
     CDS             complement(4351..6591)
                     /gene="pilQ"
                     /locus_tag="PKK95_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOC18085.1"
                     /translation="MRHNWTLQTTAGRGRAHPARGRAAAIVAGLAIVLAGHGGLRGFG
                     PADDGPPRARLSTISAQAGPLGTSVRIEASEPVAYVTARPDPMTILVELRHATAAGVV
                     NRLASARVGHVAAATVEDAVAGDGAKVARVQLLLAGPVAHHVRAERNVIQVDVGGQAA
                     TSPPAGAAGPSPEAMRQATRLESIRTASGPKGVEVTLAADGALPAAAPEFMKDKPVRL
                     FFDLAGVAPNVPEVTKVGQGPVTQVRVARRSAKPLVTRVVFDLASRVPFRVQPVGHEL
                     KVLFGDGAADAPSAPSPAAAPATAAAAPPRVPAPMQVPASISAPQAGSAPRQYTGHPI
                     SLDFQGADLRSVLRTFSEVSALNIVIDPAVQGSVDVALRDVPWDQALDIILRANKLGY
                     VVDGTIVRIAPLSVLADEESQRRKLAEEQALSGDLKVVTRALSYAKAEDLKDLLVRSA
                     LSKRGDVQFDPRTNTLILRDLPASLETAVELLEALDRPQPQVEIEARIVQTTRDFSRS
                     IGVQWGITGRVADELGNTTGLAFPNKGTLTGRTGARQGGETGSATAQAVGLGVSTATS
                     ALGLTLGSVNGAFNLDVALSALEKSGQGRVLSSPRVSTQNNMPAEMTQGTQIPIQTVA
                     NNTVTVTFKDATLTLKVTPQITAANTVIMNIQLENASPDYTRAVGTSQIPPIDTQRAI
                     TTVLVRDGETTVIGGIEVSREQAQTDRTPGLSRIPILKWLFKRDAVQDENRELLIFIT
                     PRIVKL"
     gene            complement(6592..7092)
                     /locus_tag="PKK95_07450"
     CDS             complement(6592..7092)
                     /locus_tag="PKK95_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18086.1"
                     /translation="MRGPIMTCRTGVLLALGLALGSPARAAGQAGRPAPEPAPAVQAP
                     APAPTETYSYRSEGRRDPFVSLVNRGVEPQPGQPRPTGIAALSASEVSLRGIVQSRGR
                     YLAIVQGPDARRPYIVHVNERFADGVVKAITADSMLILQEVNDPLSLTKQREIRKTLR
                     AVEEVK"
     gene            complement(7089..7727)
                     /gene="pilO"
                     /locus_tag="PKK95_07455"
     CDS             complement(7089..7727)
                     /gene="pilO"
                     /locus_tag="PKK95_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOC18087.1"
                     /translation="MAVSLSKLPWYGQVGAFLALSVAGVAAFYYLYVTPMNRQIAARQ
                     QQLNALQADIRKSQATAQRLAQFRAEVGELETRLETLKNVLPEEKDIGDLLRRLQTLA
                     VQSNLTIRGFKPGGTPVTKQLHSEFPYTLQLDGAYHNLGIFFDRVSKFPRIIHIRGVN
                     IKGKDKQEPNSTITADCVATTFVLLEPKDQEAESATATKKTPAARRTAGSRQ"
     gene            complement(7753..8337)
                     /locus_tag="PKK95_07460"
     CDS             complement(7753..8337)
                     /locus_tag="PKK95_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOC18088.1"
                     /translation="MIRINLLAVERERSKRRTGSLLQATQQKITLACSVILVLAALGI
                     GWWYWSLSKQADRIEADIVTAQRETERLRGLIAQVEQFEARRLQLQQRVALIEELRKG
                     QAGPVRLLDEISRSLPDMLWLTEIKQQGSELTISGRCTTLTALSDFVGNLELGGFFNK
                     PVEILDSQVDSQRGVGGVELIRFSVKAVVSTPAA"
     gene            complement(8334..9395)
                     /gene="pilM"
                     /locus_tag="PKK95_07465"
     CDS             complement(8334..9395)
                     /gene="pilM"
                     /locus_tag="PKK95_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOC18089.1"
                     /translation="MFGRPKPVVGLDIGSSAVKAIELRPAGRSYKVVGFGIEPIPADT
                     IVDGAIIDAGAVAEAIRRLLDGHRIKTREVAASLSGNSVIVKKINLPVMSEAELAESI
                     YWEAEQYIPFDIQDVNLDYQILTPAAAAETRGGMEVLLVAAKKEKINDYTEVVRQAGR
                     VPVVVDVDAFAVQNAYEANYDPDPSAVVVLLNAGASGVNINIVSGQQSLFTRDISMGG
                     NAYTEAVQKELSLPFESAERVKRGEPSDGVHPDEVRPILRSVTENLLLEIQKTFDFFK
                     ATAASERIDRVLISGGTSRVDGLADALADRFGTPVEPFDPFRTIQIDARKLGIEHLAE
                     VAPTSAVAVGLALRRAGDR"
     gene            9678..>10130
                     /locus_tag="PKK95_07470"
     CDS             9678..>10130
                     /locus_tag="PKK95_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18090.1"
                     /translation="MLGVLEVLEVLEVLVLEVLVLEVLVLEVLVLKVLVLKVLEVLVL
                     EVLVLGVLEVLAEAEVGGLARRSRSLPAVAAGAASVWRRRKLAGLPAGAVSEANAWRR
                     RERSERLAEAGAKRTLGGGGCWRACEANAWLRRKLAGLPAGAVSEANAW"
BASE COUNT         1349 a         3564 c         3596 g         1621 t
ORIGIN      
        1 cgcgcaaagg ggcgggccgc ttgacggaac gagcccgctg agagcgccgc ggaagccgct
       61 gcgccagggc ggcgcaggtg taccaggcgc cgccctggcg gccgtgccgt catcctcgca
      121 ggcgtcccgg gtgcttgtgg cctgcctacc ccgcctgggc gagggtgcgc gggcccgacg
      181 ccgagcgcac gaaacggttc atgaaggcgg tgttcaggcg gcccgcctgg aagtcgggtt
      241 cggcgagcac cttgaggtgc agggcgacgt tggtcttgat cccctcgatg acggtcatct
      301 cgagggtgcg ccgcatccgc gcgatggcct cctgccggtc gcgcccgtag gtcatgatct
      361 tcgcgagcag cgagtcgtag tacggggaga cggtgcactc ggagtgggcc gccgtgtcga
      421 tgcggacgcc gggcccgccg ggcagggcga agacgcggat cgtgccgggc gagggggcga
      481 aggtttccgg gcactcggcg ttgatccggc actcgagggc gtggcccgtg aaggtcacct
      541 cgctctgctt gaaggagaga cgctcgccgg ccgccacccg gatctgctcc ttgacgatgt
      601 ccaccccggt caccagctcg gtgacgccgt gctcgacctg cagccgggtg ttggcctcca
      661 tgaagtagaa gcggccgtcg ccgtccagca ggaactcgaa ggtgccggcg ttggtgtact
      721 gcacggcgcg cgcggcgtcc gcgaccaggt gccccatctt gcgccgcacc ttgtcggtca
      781 gggcgggcga gggcgattcc tcgatcagct tctggtgccg gcgctgcacc gagcactcgc
      841 gctcgccgag atgaacgacg ttgccgtgat ggtcgccgag cacctggaac tcgacgtgcc
      901 gcggcgcctc gacgtacttc tccaggtaga cgtccttcac gccgaaggcg gcctcggcct
      961 cccgctgggc ggtcctgagc gcctgcacca gctcgcccgg gtgggccgcc acgcgcatcc
     1021 cgcggccgcc gcccccggcg gttgccttca cgagcaccgg gaagccgatc tcgcgggcga
     1081 tccgcgccgc ctcctcgtcg ctctcgacga cgccctcgct gccgggcagc accggcacgc
     1141 ccgccttcct catcgcgcgg cgcgcccgcg ccttgtctcc catcagccgg atcacccggg
     1201 gatcggggcc gatgaacttg atgtggcagg cctcgcagac ctcggcgagg tacgggctct
     1261 cggagaggaa gccgtaaccg gggtggatcg cgtcggcccc ggtcagctcg gcggcgctga
     1321 tgacggccgg cacgttgagg tagctctgtc ccggccgcgg cggcccgatg cacacgtcct
     1381 cgtctgcgaa gcggacgtgc agggagtcct cgtccgcctc cgaatacacg gcgaccgtct
     1441 tgatgcccat ctcccggcag gcgtggatga cgcgcagcgc gatctcgccg cggttcgcga
     1501 tcaggacctt cttgaacatg ccccctgctt cacttcgcgc ggatggcgaa gagccgctcc
     1561 ccgtactgca ccggctggcc gttctccacg tagatcttga ccagggtgcc ctcgtactcg
     1621 gagtcgatgt tgttcatcat cttcatggcc tcgacgatgc acaacgtctg ccccttccgc
     1681 acggggtctc ccacctggac gaacgggggg gcgttcgggg ccggggcccg gtagaacgtg
     1741 ccgacgatcg gcgacttgac caccgccagt tcgctgtcgg cctcgaggtc ttccccggcc
     1801 ggggcgggag cgggcggcgc gacgggaacc ggcgcggggg cgggaggcag ggcgaacggc
     1861 gcggcggagg acgccgccgg ctgcttgcgg atgcgcaggc gccggccgtc ggcctcgagc
     1921 tcgagctcgg ccaaatcgtg ctcgcgcgcc atttccagta tctgccgcaa ctcctcgagc
     1981 ttgatcatcg acacctcggc acccggacgg atcggagcat ggtatcaaat ccgtccctcc
     2041 gaacacagcc gtggggcgct gcgggggtgc gggtcagtgg ccgtccggcc cttcgggggc
     2101 ggccgcccgc cgttcgcggt cggcgatgat cgcctcgagc cactcctcga gcgcctcggt
     2161 ggccgggccc gcgggcgggg cggcagccgg ctcggaggag ggcagggcgg cggcggccgg
     2221 ctcggaggcc gccaggacgg ttggagccgg gtcgggatcg gtggcgtgcg gttcttcctg
     2281 cagggactcg accgggacgc ccgggtcggg cgcggcttcg acgtccggcc cgggtgaggc
     2341 cggcgcctcg cccgcgatcg acagcagctc gcgctggaca tcctcgaacg agaggccgtc
     2401 cacgacggcg ggcgtccgcg tggccggctc gagctgtccc tggatctcct cgacgaggcg
     2461 ttcgagctcc gggtcgtgcg gcgcgaactc gagcgccgtg cggtagtgcg ccagcgcctc
     2521 gtgcagctcg ccgcggcggt ggtggatgtc ggccagcccg cgcagggccg ccaggttctc
     2581 gggcgccgtg cgcagcacct gcttcagctc ggcctgcgcc gcgtccagtt cgccgacctc
     2641 cagcagcgcg cgtcccaggg tgacgcgggc cgacaggtag ccgggatgcc gggcgaggcc
     2701 ggtctcgcag atctcgaccg cctcctgcag gcgtccggcg cgccggtact cctcggcgag
     2761 ctgggcgaag gcgatcgagg ccgggtcctt ctggacgcgg cggcgaagct cctcgatgcg
     2821 ggcggaatcg gccacggttc tccccagggt gtgacgcgct actcggtgac gtaaacggtc
     2881 cgggtcgcgc tcgacaccga cccggcgctg tcggtgacgc tcagcttcac cgtgaagctg
     2941 ccggacttct cgtacacgtg ggtcgtctgc gagctgtcgc ggtgccggtg cacgtgaccg
     3001 tcaccgaagc tccactcgta cacgtcgatc gtccttccgg tcttcgtcga cgaggacgag
     3061 ccgtcgaacg acaacaggtc tcccaccttc ggcgcttcgg gcgcgagggt gaagaacgcc
     3121 cccgaggctc ccttgatgac cccgggcggc accagccgca cctggacctg gcgcgaaacg
     3181 gcggtcgagt agtcggtccc gagcggggtc acgaggatcg tgacgacgtc gctgtcgagc
     3241 gagttggcct gcgccttcgg cggcgtgtag gtgacgaccg ccttgccgtc cgacccggtg
     3301 acgatgttgt gccccggcga cagggtgccg tagtcgacca gcctggactg gaaactgatc
     3361 tcgacgcgga gcgcgaggtt gcgcaccggc tgcgagttcg ggtcgcgggc cgtcacgacg
     3421 atctgcgccc gcgactcgcc gtcctggatc acgacgtcgg gcacggcctg cagcgccagc
     3481 gacaggccga gctcggatgg cccggccagg ggcggcgtgt ccgtgttctt caccgtgcag
     3541 gccgccaccg acagggcgac cgcaaccacc agcgtgcacg cgcgcaatgc tctcagcata
     3601 tggactgccc cggtcgttct tccacccgtc atccggacct ggccggcgcc cgaacgccgg
     3661 ccccctgacg ccggccgaag ccggccgccg cgtcgctact cgggatcggc ccagtcggcg
     3721 aagttcacgc cgatctgccc cgtggccttg acggcgttgc ccgcctggtc ctgcccgtag
     3781 aaggtcacct cggcgatcgt cgagatgagc tgcgccccgc ccatgcgccg cagcgccagc
     3841 agcggcgcct cgcgcttggc ctgcgccctc accagcacga agctgagcct ggccccgctc
     3901 gggccgaagg tggccgtggc ggcgccgtcg aactcgtacg gcacgtcgac gcccggcgtg
     3961 ttccggccgt ccgcgcggac gaaccgcacg tggtaccggt tgatcgtgat ctggttcgcc
     4021 tcgctcggct gggtcgggct gcccggcgtc ccgggatcct tgaggacgat gtgcgcctcg
     4081 agctcgcccc ggtcctcgta gaaggtctcc gcgaagtacg tgttctcgcc ctcggtgacc
     4141 ttgatgttgg tgaccacgtc ggaatcgagg gtgtggtcga agtcgtcggg ctcggcaccg
     4201 gacgccccca tcaggttgtc gatcaccagg taggcgggcg accgcccctg ccgcaccacg
     4261 tcgccgcacg aggccgacgc gagcgccacg aagaggacca gccccgcccc cgccgccttc
     4321 cgatatcccc agcaagtcat cggcgttccc ctacagcttc acgatcctcg gcgtgatgaa
     4381 gatcaacagc tcgcggttct cgtcctgcac cgcgtcgcgc ttgaagagcc acttcaggat
     4441 cgggatccgg ctcagccccg gcgtccggtc cgtctgcgcc tgctcccggc tcacctcgat
     4501 gccgccgatg acggtcgtct cgccgtcgcg cacgagcacc gtcgtgatgg cgcgctgcgt
     4561 gtcgatgggc gggatctggc tggtgcccac cgcgcgcgtg tagtcggggg aggcgttctc
     4621 gagctggatg ttcatgatca ccgtgttcgc cgcggtgatc tgtggcgtga ccttcagcgt
     4681 gagcgtcgcg tccttgaagg tcaccgtcac cgtgttgttc gcgactgtct ggatcgggat
     4741 ctgcgtgccc tgcgtcatct ccgccggcat gttgttctgg gtggacacgc gcgggctcga
     4801 cagcacgcgg ccctgccccg acttctccag ggccgagagc gccacgtcca ggttgaacgc
     4861 gccgttcacc gagccgagcg tgagccccag ggcgctggtg gccgtcgaga cgccgaggcc
     4921 gacggcctgc gcggtcgcgc tgccggtctc tcctccctgc ctcgccccgg tgcgccccgt
     4981 cagggtgccc ttgttcggga aggccagtcc ggtggtgttc cccagctcgt ccgccacccg
     5041 tcccgtgatg ccccactgca cgccgatcga ccgcgagaag tcgcgggtcg tctggacgat
     5101 tcgcgcctcg atctcgacct gcggctgcgg gcggtcgagc gcctcgagca actccaccgc
     5161 cgtctcgagc gaggccggca gatcccgaag gatcaacgtg ttggtgcgcg ggtcgaactg
     5221 cacgtcgccg cgcttgctga gcgcgcttcg taccagcaga tctttcaagt cttcggcctt
     5281 ggcgtagctc agggcacggg tgaccacctt gaggtcgccc gagagcgcct gctcctccgc
     5341 cagtttccgg cgctggctct cctcgtcggc gagcaccgag agcggcgcga tccgcacgat
     5401 cgtcccgtcc acgacgtacc cgagcttgtt ggcccgcagg atgatgtcga gggcctggtc
     5461 ccacgggacg tcgcgcagcg ccacgtctac cgacccctgg accgccgggt cgatcacgat
     5521 gttcagcgcg ctcacctcgg agaaggtgcg cagcaccgag cgcaggtcgg cgccctggaa
     5581 atcgaggctg atcgggtggc ccgtgtactg ccgcggggcg ctcccggcct gcggcgcgct
     5641 gatcgaggcg ggcacctgca tcggcgccgg gaccctgggg ggcgccgccg cggccgttgc
     5701 gggcgccgcc gctggcgagg gcgcggacgg agcgtcggcc gccccgtcac cgaacagcac
     5761 cttcagctcg tggccgacgg gctggacccg gaacggcacc ctggacgcca ggtcgaacac
     5821 gacccgggtg accagcggct tggcactccg ccgcgcgacc cggacctgcg tgaccggccc
     5881 ctgcccgacc ttggtcacct cgggcacgtt cggcgccacg cccgccaggt cgaagaagag
     5941 gcggacgggc ttgtccttca tgaactccgg cgcggcggcg ggaagggcgc cgtcggcggc
     6001 cagcgtcacc tcgaccccct tcggtcccga ggcggtccgg atcgactcga gccgcgtggc
     6061 ctgccgcatc gcctccggcg acgggcccgc cgcgccggcg gggggtgagg ttgccgcctg
     6121 gccgcccacg tccacctgga tcacgttgcg ctcggcgcgc acgtggtggg cgaccgggcc
     6181 cgccagcagc aactggacgc gggcgacctt cgcgccgtcg cccgcgacgg cgtcctcgac
     6241 ggtggcggcc gcgacgtggc ccacccgcgc cgaggccagc cggttgacga caccggcggc
     6301 cgtggcgtgg cgcagttcga ccaggatggt catcgggtcg ggacgcgccg tcacgtaggc
     6361 gaccggctcg gatgcctcga tccgcaccga cgtgccgagc gggcccgcct gggcggagat
     6421 cgtgctcagc ctcgcgcggg gcggcccgtc gtcggccggc ccgaacccgc ggagcccgcc
     6481 gtgaccggcc agcacgatgg ccagtcccgc gacgatcgcc gcggcgcgcc ccctcgccgg
     6541 gtgcgcacgt ccccgtcccg ccgtagtctg gagagtccag ttgtgccgca tctacttgac
     6601 ctcttcgacc gcccgcaagg tcttccgaat ctcacgctgc ttggtgagcg acaacgggtc
     6661 gttcacttcc tggaggatga gcatcgagtc ggcggtgatt gccttcacga cgccgtcggc
     6721 gaagcgctcg ttcacgtgga cgatgtaggg ccggcgggcg tcgggtccct ggacgatggc
     6781 caggtaccgg ccgcggctct gcacgatccc gcgcaggctc acctcgctcg ccgagagggc
     6841 ggcgatgccg gtgggacgtg gctgccccgg ctgcggctcg acgccccggt tgacgaggct
     6901 gacgaaggga tcgcgccgcc cctccgaccg ataggaatac gtctcggtgg gcgccggggc
     6961 gggcgcctgc accgcgggcg ccggctcggg ggccggccgc cccgcctggc cggccgcccg
     7021 ggcgggcgaa ccaagcgcga gccccagggc caacagcact cccgtccggc acgtcatgat
     7081 cgggcccctc attgccggct ccccgccgtt cggcgcgcgg ccggcgtctt cttcgtcgcc
     7141 gtcgcgctct cggcctcctg atccttcggc tcgagcagca cgaaggtggt cgcgacgcag
     7201 tcggccgtga tcgtcgagtt cggctcctgc ttgtccttgc ccttgatgtt cacgccgcgg
     7261 atgtggatga ttcgcgggaa cttgctcacc cggtcgaaga agatgccgag gttgtggtag
     7321 gcgccgtcga gctgcagcgt gtacgggaac tccgagtgca gctgcttggt gaccggcgtc
     7381 cccccgggct tgaacccgcg gatcgtcagg ttcgactgca ccgccagcgt ctgcagccgg
     7441 cgcagcaggt cgccgatgtc cttctcctcg ggcagcacgt tcttgagcgt ctcgaggcgc
     7501 gtctcgagct cgcccacctc cgcgcggaac tgcgcgagcc gctgcgccgt cgcctggctc
     7561 ttgcggatgt ccgcctgcag cgcgttcagc tgctgctggc gggcggcgat ctgccggttc
     7621 atcggcgtga cgtacaggta gtagaaggcc gccacgcccg ccaccgacag ggccaggaat
     7681 gcgccgacct gtccgtacca cggcagcttc gacagtgaca cagccacggg cttccctttc
     7741 ctgatgcgcc tgctacgcgg cgggggtgct gacgaccgct ttcaccgaga accggatcaa
     7801 ctcgacgccg ccgacgccgc gctggctgtc cacctggctg tcgaggatct ccaccggctt
     7861 gttgaagaac cctcccagct ccaggttgcc gacgaagtcg gacagcgccg tcagcgtggt
     7921 gcaccgcccg ctgatcgtca gctcgctccc ctgctgcttg atctcggtca gccagagcat
     7981 gtccggcagg ctgcggctga tctcgtcgag cagccggacc gggccggcct gccccttgcg
     8041 cagctcctcg atcagcgcca cgcgctgctg gagctggagg cggcgcgcct cgaactgctc
     8101 gacctgcgcg atgaggcccc gcagccgctc ggtttccctc tgggcggtca cgatgtcggc
     8161 ctcgatccgg tcggcctgct tgctcaacga ccagtaccac cagccgatgc cgagggcggc
     8221 caggaccagg atgacgctgc acgcgagcgt gatcttctgc tgcgtcgcct ggaggaggct
     8281 gcccgtgcgg cgcttggaac gctcgcgttc gacggcaagg aggttgattc gaatcatcgg
     8341 tcgcccgccc gtctgagcgc caggcccacc gcgaccgcgg aagtcggcgc gacttccgcg
     8401 aggtgctcga tccccagctt cctggcgtcg atctggatgg tgcggaacgg gtcgaacggc
     8461 tccaccgggg tgccgaaccg gtcggccagc gcgtcggcca ggccgtccac ccgcgaggtg
     8521 ccgccgctga tcagcacccg gtcgatccgt tccgaggccg ccgtggcctt gaagaagtcg
     8581 aacgtcttct ggatctcgag cagcaggttc tcggtcaccg agcgcaggat cggccggacc
     8641 tcgtcgggat gcaccccgtc gctcggctca ccccgcttga cgcgctcggc gctctcgaag
     8701 ggaaggctca gctccttctg caccgcctcg gtgtaggcgt tgccgcccat cgagatgtcg
     8761 cgcgtgaaca gcgactgctg cccgctcacg atgttgatgt tcacgccgct ggcgcccgcg
     8821 ttgagcagca cgaccacggc cgacgggtcg gggtcgtagt tcgcctcgta cgcgttctgc
     8881 acggcgaagg cgtcgacgtc gacgaccacc ggcacgcggc ccgcctggcg gacgacctcg
     8941 gtgtagtcgt tgatcttttc cttcttcgcc gcgaccagca gcacctccat ccccccgcgg
     9001 gtctcggccg ccgccgccgg cgtcaggatc tggtagtcca ggttgacgtc ctggatgtcg
     9061 aaggggatgt actgctcggc ctcccagtag atcgactcgg ccagctcggc ctcgctcatc
     9121 accgggaggt tgatcttctt gacgatgacc gagttgcccg agagcgacgc ggccacctcg
     9181 cgcgtcttga tgcgatggcc gtccaggagc cggcggatcg cctcggcgac cgcgccggcg
     9241 tcgatgatcg cgccatcgac gatggtgtcg gccgggatcg gctcgatccc gaagcccaca
     9301 accttgtacg accggccggc gggccgaagc tcgatcgcct tgaccgcgct cgacccgatg
     9361 tccagaccga caacgggctt gggtctgcca aacacgcagt gtccccttct cggaaactcg
     9421 gaaacgcggg tgcccgggag cggggcaccg accagaacca ccccagccat tccgcaagcg
     9481 gggtgccaat caaaaaaccg cccgaaatcg ccgattctct gccccgcgcg tgcagaactt
     9541 gccaccgagc ggcggcagcc gctggcggtt cccgcacagc tttgggcggc cgattacaaa
     9601 tctgacgcgc ccatccccga agcaaacctg ttatcggcca ctgctcaggt tggggacagg
     9661 ggggactgaa tcttgtcgtg ctgggggtgc tggaggtgct ggaggtgctg gaggtgctgg
     9721 tgctggaggt gctggtgctg gaggtgctgg tgctggaggt gctggtgctg aaggtgctgg
     9781 tgctgaaggt gctggaggtg ctggtgctag aggtgctggt gctgggggtg ctggaggtgc
     9841 tggcggaggc ggaagttggc gggcttgccc gccggagccg tagcctgccc gccgtagctg
     9901 cgggcgcagc gagcgtttgg cggaggcgga agttggcggg cttgcccgcc ggagccgtga
     9961 gcgaagcgaa cgcctggcgg aggcgggagc gaagcgaacg cctggcggag gcgggagcga
    10021 agcgaacgct tggcggaggc gggtgctggc gggcttgcga agcgaacgct tggctgaggc
    10081 ggaagttggc gggcttgccc gccggagccg tgagcgaagc gaacgcctgg
//
LOCUS       DAOPQX010000104        10064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7606_length_10064_cov_5.401139, whole genome shotgun sequence.
ACCESSION   DAOPQX010000104 DAOPQX010000000
VERSION     DAOPQX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7606_length_10064_cov_5.401139"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1939)
                     /locus_tag="PKK95_07475"
     CDS             complement(<1..1939)
                     /locus_tag="PKK95_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOC18091.1"
                     /translation="MNGFLRIVAALICLAAFTVPTVSAQISSGIWVDKNELQSLPMSG
                     TAWNNLKSAADKSCGTPDLENQDDKVNTCVMAKALVYARTRTSSYASGVATALRSIVN
                     SGTYGGRALALGRELAAYVIAADLIDLRTYDPSLDSSFRSKIRSLLTASTNDGPSNLV
                     DCHEKRPNNWGTHCGGSRAAVAAYLGDTAELARTAQVFKGWLGDRASYAGFKYGDLAW
                     QYNSSSPVGVNPRGATKSGYNIDGVLPDDQRRAGGFTWPPPKENYAYEALQGALAQAV
                     ILDRAGYDVFNWSDRALLRAFTWLHEQADFPAGGDDEWQPWVINYFYGSSFPEPSSTQ
                     PGKNVGWTDWTHSGATSSCSFSVSPTSASLPASGGGTQASVTTTSSCAWTAGSNVSWI
                     SLNRSSGTGSGTVIATVAANSSSSSRTGSFVIAGRTFTVSQAGTTTTPPAAPAAPSAP
                     SPAAGASSVSTATTLRWTCSGASSYSVRFGTSNPPAQVATGLATTTYTPSALTAGRTY
                     YWQVVGVNASGSTAGPVWSFSTATATTPPPSSAMPSPWTAGDLGSVGLAGSAAYSNGV
                     FTVTGSGAGMTGSADAFHFVRQPLTGHSTITVRLTGLQASGSLAQAGIMMREGKGGVT
                     AGSAHATLAVRPNGSVVFLART"
     gene            complement(2348..3127)
                     /locus_tag="PKK95_07480"
     CDS             complement(2348..3127)
                     /locus_tag="PKK95_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC18092.1"
                     /translation="MAESVHGRVLWYELMTRDVKGAEAFYTDVVGWSVSPFEGSSPEG
                     YYGVWSRKDGTGIGGVMAIPEGMTFPPHWGMYVGAPKLEEAVASIERLGGGAVTGVIE
                     VPQVGRMRTMRDPQGAAFSLLEPAMPGVPPDREPGIGDASWHELYTSDPEAALKFYSE
                     MFGWAPTEALDMGPMGKYHMFGRGQGFGGGIMKKPAEMQAPPAFWGFYFRVPDVHAAV
                     ERVKAGGGQVLNGPMEVPGGDWVANCMDPQGAAFGLHHKKK"
     gene            3268..4269
                     /locus_tag="PKK95_07485"
     CDS             3268..4269
                     /locus_tag="PKK95_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOC18093.1"
                     /translation="MQAARLWGRRDLRIGDEEKPWPAADEELVRVTAVGICGSDIHWF
                     DEGGIGGTNVVRPLVPGHEFAGVTPDGVRVAVDPAIPCRTCELCRRGHPNLCERVVFA
                     GHDVDGALREWIAWPRRNLHPLPGSFSDADGAMLEPLGVSIHACHLAHIEPGARVGVF
                     GCGPIGWLTIQLALHRGASQVIATELASRPHRLAAAAEKGATTVAADGFEAAAIRDLT
                     GGYGLDVAIEAAGDNAAVDAAVDAVRPGARIVLAGIPSDPRTSVSASSARRKGLTLAW
                     ARRMKETYPEAIGLVSSGAIDVRSGVTHTFTLAEAARAFEAAARREGMKVMVGLRRG"
     gene            complement(4319..5749)
                     /locus_tag="PKK95_07490"
     CDS             complement(4319..5749)
                     /locus_tag="PKK95_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC18094.1"
                     /translation="MSGADGEPDRAERARDLSQRLADHVVGQREALERIVPYLQMYEA
                     RLNPPDRPAGIFLLLGPTGTGKTRTAEAVAEVLHGSPKHLLKVDCAEYQSDHEVAKLI
                     GAPPGYVGHRETKPILTQERLLGAVTPGCDLAVVLFDEIEKAAPAFTALLLGILDRGT
                     LRLGDNTAVSFEKSLVFLTSNVGARQMLNVVRPAIGFQGVERRSPASIAARLRSAGME
                     AVRRRFSPEFVNRLDAVITYRPLDAAALAGILDHHVAELQEHVHSRLRERSFDIVVEP
                     AARELLLARGTSDEFGARELKRTVHRMLTQPLAALVADSGVPPGGAVRVSVSEDGESL
                     ALRAEEPAPASPHPAASGPVVLVIDENDDLRRMLGHALSGVGIEPLTAGSAARARELA
                     AAQSPDLLVVDLMLPDGEGLSLALELLAADPRLRVVVTSGAELSADEAALCEQHGFPV
                     LRKPFLPEDAVSLVRAGLQQSKAAGR"
     gene            6021..7352
                     /locus_tag="PKK95_07495"
     CDS             6021..7352
                     /locus_tag="PKK95_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC18095.1"
                     /translation="MKTTIDRREFCKYGAMGIVGAAVSPSLAAFGGQAARTRVAIVGT
                     GIRGTTTWGMNLLRDVGGRVEIVGLCDVNPDRVKVAQRWIGGAIPTFTDFDEMIRSTK
                     PERVIVTTVDATHSEYVCRAMELGIDPICEKPLCTHDFQAQQILDTQRRTGRRLDVTF
                     NARHEAGAMKVKELLLAGEIGRLYSVSYEEFLDLDHGASYFRRWHGLKQASGTLLCHK
                     ASHHFDQLNWWIDADPVEVTAEGRLNKYGANGRLRHANCRLCPHKKQCDLYWDMTTSK
                     QLMEMYADCEDQDGYYRDGCLYRREINVPDTYSVQIRYSNQVLVTYSLNATVPYEGQS
                     IVFNGSKGRLDVRNYNRQPWQVPHESEIRLTRNFKDSTVITVDPQRGEFFEHGGADQR
                     IKRMIFDTSVPDPLSQRAGLRAGILSSAVGIAGYTSIETGRKVRIDEVVKL"
     gene            complement(7420..9438)
                     /locus_tag="PKK95_07500"
     CDS             complement(7420..9438)
                     /locus_tag="PKK95_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC18096.1"
                     /translation="MIPTTLFASLAAAVLLSAAEGPDAVSRQGAEPAPATLDVTAAGR
                     DGRFVEGLGAGDFIVTVDGTRRTVLWVRRVSRGPGAATEAASRVDGSGGIELAAELSR
                     TVLVVVDQSSLLRGDERAIVQAAGLFLDRLGLNDRVAVVTLPMAPAALLSFTPERAEV
                     RAALARVTGRLEPAAVQAQAEPIPTDAPEQATMSGLKPEAQIKFDPRTGNSPAADVSE
                     GPDTLGAMASLLDSLAAVPGRKVVAVFSAGLAAASGAQLDAAARAAAASRAVVFGFGA
                     RTTGGRDAGRSLTTAPLERLAAATGGTFARLGRDPARAVDAVIGSLGACYVLGIGTAS
                     DPGRAGRHRLEVATTRKGITVRAASWLMASPAPGDRVLEAGPAALPADPAAASGEPDA
                     TQAAAGRKLAAREARDEAERLVLLARMFDYADGYERQYSMLVAEEDYRQSASAGGVHL
                     KSDVLLVRPDEAGDWISFRDVFEVDGKPVRDREERLRRLFLDPSPDARARLEVLGEES
                     ARYNVGWVERTANVPLLPLSFLRAANRGRFAYELAGRDRVGGIEAVRVRYTEQARPTL
                     IGDRLHQDVPVFGSFLVDAATGAVVETRMNASRGDARAEIVVRYRADPALGLWVPAEM
                     REKYSQPDIGYSGRSGLIRRAVVEGRATYSKFRRFQVTTGEEVKEPRK"
     gene            complement(9435..>10064)
                     /locus_tag="PKK95_07505"
     CDS             complement(9435..>10064)
                     /locus_tag="PKK95_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18097.1"
                     /translation="YSAVFGGRYADAERFLRENAWMASALGLPPMDTRIALAVVFPEL
                     VRFSALEDAVQVRGLKVLYVQYGRAYSDFSVGRFQMKPSFAEQVESDYARLFGRRERE
                     AVGLAAFGHGDTAALREQRVRRLDDLEWQVRYLRLFMLVMQKRYPAVSRKGPDERLRF
                     YATAYNTGYAAGEAAIRRRMGRRRFHVARFASSDTYDYADVALFAFRRQ"
BASE COUNT         1542 a         3567 c         3523 g         1432 t
ORIGIN      
        1 ccgtccttgc gaggaagacc accgagccgt tggggcgaac ggccagggtt gcgtgcgccg
       61 acccggccgt cacgccgccc ttgccctcgc gcatcatgat gcccgcctgc gccagcgagc
      121 cgctcgcctg caggcccgtg aggcgcacgg tgatcgtgct gtgaccggtc agcggctggc
      181 gaacgaagtg gaaggcgtcg gcgctgccgg tcatgcccgc accgctgccc gtcaccgtga
      241 acacgccgtt cgagtaggcg gccgacccgg cgaggcccac gctgccgaga tcaccggccg
      301 tccaaggcga cggcatcgcg gagctcgggg gcggcgtggt cgcggtcgcg gtcgagaacg
      361 accacaccgg ccccgcggtc gaacccgacg cgttcactcc aacgacctgc cagtagtagg
      421 tgcggccggc cgtcagcgcc gacggcgtgt acgtcgtggt cgcgagaccc gtggcgacct
      481 gcgccggcgg gttcgacgtg ccgaagcgca cggagtagct gctggccccc gagcacgtcc
      541 agcgcagcgt cgtcgcggtg ctgacggagc tggcgccggc agcaggcgac ggggcggagg
      601 gcgcggccgg agccgccggc ggcgtggtgg tcgtacccgc ctggctcacc gtgaaggtcc
      661 ggcccgcgat cacgaagctg ccggtccggg aactgctgga ggaattggcc gcgacggtcg
      721 cgatcacggt ccccgatccg gtcccgctcg acctgttcag cgagatccag gagacgttcg
      781 acccggcggt ccacgcgcag ctgctggtgg tcgtcacgct cgcctgcgtg ccgcctcccg
      841 acgcgggcag cgaggccgag gtcggcgaca ccgagaacga gcacgacgag gtggcgccgc
      901 tgtgcgtcca gtcggtccag ccgacgttct tgccaggctg cgtggacgag ggctccggga
      961 acgagctgcc gtagaagtag ttgatcaccc acggctgcca ctcgtcgtcg cccccggccg
     1021 ggaagtcggc ctgctcgtgg agccacgtga acgcgcgcag cagcgcccgg tccgaccagt
     1081 tgaacacgtc gtaccccgcg cggtcgagaa tgaccgcctg ggcgagcgcg ccctggagcg
     1141 cctcgtacgc gtagttctcc ttcggcggag gccaggtgaa cccgccggcg cggcgctggt
     1201 cgtccggcag cacgccgtcg atgttgtacc ccgacttggt ggcgcccctg gggttcacgc
     1261 ccaccgggga cgagctgttg tactgccacg ccaggtcgcc gtacttgaag cctgcgtagg
     1321 aggcgcggtc accgagccag cccttgaaga cctgcgcggt ccgcgccagt tccgcggtgt
     1381 cgccaagata cgccgcgacc gcggcgcgcg acccgccgca gtgggtgccc cagttgttgg
     1441 ggcgcttctc gtggcagtcc accaggttgc tgggcccgtc gttggtcgac gcggtcagca
     1501 gcgaccggat cttcgaccgg aacgacgaat cgagactcgg atcgtaggtc cggaggtcga
     1561 tcaggtcggc ggcgatgacg tacgcggcca gctcgcgccc gagcgccagc gcccttccgc
     1621 cgtaggtccc gctgttcacg atcgaccgca gggccgtcgc cacgccggac gcgtagctgg
     1681 atgtgcgggt ccgcgcgtag acgagggcct tcgccatcac gcacgtgttg accttgtcgt
     1741 cctggttctc caggtctggc gtcccgcagc tcttgtcggc cgcggacttg aggttgttcc
     1801 aggccgttcc agacatcgga agcgactgga gctcgttctt gtcaacccag atccccgacg
     1861 agatctgtgc ggagacggtc ggcaccgtga aggcggctag acagatgagt gcagcgacga
     1921 tgcggaggaa accattcact tggactgctc cctgcaagga tgcccgcgcg cggcgcggaa
     1981 caggcttctg ggccccggcc ccgcgtccgc ccgcgcagga cggaccagga ccgggaatga
     2041 tcgggtgctt gtctgtgagt cggggggcgt cgcgagtcgc ctatcgacct tgtacgcgtt
     2101 gactacatgc gcggcgcaat gagggattgc cagattcggg ccgtcggagt ccagagccga
     2161 atcatgagga ttttgcgcag gaaacccgcc cttgtcgcac cctggcgcga caaagctggg
     2221 ttcccgattt caatcctgct gctgaggtga cttggacgag cggaccgagg cgcccaggcg
     2281 ggagagacgt gcgagccggg gggtgaagga gcagggccga agccctgctc cagcgtgaaa
     2341 cgccgcccta cttcttcttg tggtgcaggc cgaaggcggc cccctgcggg tccatgcagt
     2401 tggccaccca gtcgccgccc ggcacctcca tcggaccgtt cagcacctgc ccgccgcctg
     2461 ccttcacgcg ctcgacggcg gcgtgcacgt cgggcacgcg gaagtagaag ccccaaaagg
     2521 ccgggggcgc ctgcatctcc gcgggcttct tcatgatgcc gccgccgaag ccctggcccc
     2581 ggccgaacat gtggtacttc cccatcggcc ccatgtcgag cgcctcggtc ggcgcccagc
     2641 cgaacatctc gctgtagaac ttcagggccg cttcaggatc gctggtgtac aactcgtgcc
     2701 acgacgcgtc gccgatcccg ggctcgcgat ccggcggaac tccaggcatg gcgggctcca
     2761 gcaacgagaa cgccgcgccc tggggatccc gcatcgtccg cattcgcccg acctgcggca
     2821 cttcgatcac gccggtcacc gcgccgccgc caagccgctc gatggaggca accgcctcct
     2881 cgagcttcgg cgcgccgacg tacatccccc agtgcggggg aaaggtcatg ccctcgggaa
     2941 tcgccatcac gccgccgatg cccgtcccat ccttgcgcga ccacaccccg tagtaccctt
     3001 ccggcgacga gccctcgaac ggcgataccg accacccgac gacgtcggtg tagaacgctt
     3061 cggcgccctt cacatccctc gtcatcaact cgtaccagag cacgcggcca tgcacggatt
     3121 cggccatcga cacttctcct tccggcgctc gccggggtgg taggttggcg cttcgaaaac
     3181 ccgtccccca gtgtcacaca actcgcgccg cggacggccc cgggaagtcg tgtatcggct
     3241 aagatccgga agcaagaggg ggaacggatg caggcggcgc gactctgggg acggcgggac
     3301 ctgcggatcg gggacgagga gaagccctgg ccggcagcag acgaagaact ggtccgcgtg
     3361 accgccgtgg gcatctgcgg ctccgacatc cactggttcg acgagggagg gatcggcggg
     3421 acgaacgtcg tgcgcccgct cgtccccggc cacgagttcg ccggcgtgac gccggacggc
     3481 gtgcgcgtcg cggtcgatcc cgccatcccg tgccggacgt gcgagctgtg ccgccgcggc
     3541 catcccaacc tctgcgagcg cgtcgtcttt gccggccacg acgtggatgg cgcgctgcgg
     3601 gagtggatcg cctggccccg ccgcaacctc cacccgctgc ccggctcgtt ctccgacgcc
     3661 gacggcgcga tgctggagcc gctcggggtg tcgatccacg cctgccacct cgcccacatc
     3721 gagcccggcg cgagggttgg cgtgttcggc tgcggcccga tcggctggct gacgatccag
     3781 ctcgccctcc atcggggcgc ctcgcaggtc atcgcgaccg agctcgcctc gcgcccgcac
     3841 cggcttgccg ccgccgccga gaagggggcg acgacggtcg cggcagacgg cttcgaggcg
     3901 gcggccatcc gcgacctcac cggcgggtac ggcctcgacg tggcgatcga ggcggccggc
     3961 gacaacgccg ccgtcgatgc cgcggtcgac gcggtccggc ccggcgcgcg gatcgtcctc
     4021 gcggggatcc cgagcgatcc gcgtacctct gtcagcgcct cgagcgcgcg ccgcaaggga
     4081 ctgacgctcg cctgggcgcg gcgcatgaaa gagacctacc ccgaagcgat tggcctggtc
     4141 tcgagcggcg ccatcgacgt ccgctcgggc gtgacgcaca ccttcaccct ggccgaggcc
     4201 gcgcgcgcct tcgaggcagc tgcacgccgc gagggaatga aggtgatggt cggcctgcgc
     4261 cgcggttagc cgagggctga accggagctt gtgctgtaag cgtctctcga gcggcctgtc
     4321 agcggccggc ggccttggac tgctgcaggc cggcgcgaac gaggctcacc gcgtcctcgg
     4381 gaaggaacgg ctttctcagc acagggaagc cgtgctgctc gcacagtgcc gcctcgtctg
     4441 ctgacagctc ggcaccgctc gtcaccacga cccgcagccg cgggtcggcg gccagcagct
     4501 ccagcgccag cgacagcccc tcgccgtcgg gcagcatcag gtcgacgacg aggagatcgg
     4561 gcgactgagc ggctgccagt tcgcgcgccc gcgcggcgct gccggccgtc aggggctcga
     4621 tgcccacgcc cgacagcgcg tgcccgagca tccggcgcag gtcgtcgttc tcgtcgatca
     4681 ccaggaccac cgggcccgag gccgcggggt gcggagaagc tggcgccggt tcttcggccc
     4741 gcagggcgag actctccccg tcctccgaca cgctgacccg caccgcccct ccgggcggga
     4801 cgccgctgtc ggccaccagc gccgcgagcg gctgcgtcag catgcggtgc acggttcgct
     4861 tcagctcgcg cgcgccgaac tcgtcgctcg tcccccgcgc gagcagcagt tcgcgggcgg
     4921 ccggctcgac gacgatgtcg aacgaacgct cgcggaggcg gctgtgaacg tgctcctgca
     4981 gctcggccac gtggtggtcg aggatcccgg cgagagcggc ggcgtcgagc gggcggtagg
     5041 tgatgaccgc gtcgaggcgg ttcacgaact cgggcgagaa acggcgtcgc accgcttcca
     5101 tgcccgccga ccgcagccgg gcggcgatgc tggccggcga ccggcgctcg acgccctgga
     5161 agccgatcgc cggacgaacc acgttcaaca tctggcgggc gccaacgttg ctcgtcagga
     5221 acaccaggct cttctcgaag ctcaccgcgg tgttgtcgcc gaggcgcagc gtcccgcggt
     5281 cgaggatccc cagcagcagc gcggtgaagg ccggcgccgc cttctcgatc tcgtcgaaga
     5341 ggacgacggc aaggtcgcat ccgggcgtca cggcgccgag gagccgttcc tgcgtgagaa
     5401 tcggcttggt ctcgcggtgg cccacgtagc cgggcggtgc gccgatcagc ttggcgacct
     5461 cgtggtcgga ttggtactcg gcgcagtcga ccttcagcag gtgcttgggg ctgccgtgca
     5521 gcacctcggc gacggcctcg gcggtgcggg tcttcccggt gcctgtgggg ccgaggagca
     5581 ggaagatgcc ggccggccgg tctggaggat tgagccgcgc ctcgtacatc tgcaggtacg
     5641 gcacgatcct ctccagcgcc tcgcgctgcc ccaccacgtg gtcggcgagt cgttgggaga
     5701 ggtcgcgcgc acgctcggcc cgatccggtt ccccgtcggc tccgctcatt gggtttggga
     5761 tccctcgagc gattatagcg tgccccggaa aaggaacctc agcgtgaggc gccggcggag
     5821 gccccccaga tcagcgcggg ctcagcggtc gcatccgaga cgcgcgtgac gcggttgaac
     5881 cagcggtaca tcgcctcgcg accgccctgg atggggcggg tacacaggcg ggacgcctgt
     5941 gctactggct gctgtgccac tggtggtcac tgctcacagg cgggacgcct gtgctactca
     6001 cttctgttag agtgtgcggc atgaagacga caatcgaccg gcgggagttc tgcaagtacg
     6061 gcgcgatggg gatcgttggc gcggcggtct cgccttccct cgcagccttc ggcgggcagg
     6121 cggccaggac acgggtggcc atcgtcggca cgggcattcg cgggaccacg acctggggga
     6181 tgaacctgct gcgcgacgtg ggcggccgcg tcgagatcgt gggcctgtgc gacgtcaacc
     6241 ccgaccgcgt caaggtggcc cagcgctgga tcggcggcgc catcccgacc ttcaccgact
     6301 tcgacgagat gatccgctcg acgaagcccg agcgggtgat cgtgaccacg gtggacgcca
     6361 cgcactccga gtacgtgtgc cgggcgatgg agctgggtat cgatccgatc tgcgagaagc
     6421 cgctctgcac gcacgacttc caggcgcagc agatcctgga cacgcagcgg cgcaccggcc
     6481 gcaggctgga cgtcaccttc aacgcccgcc acgaggctgg cgcgatgaag gtcaaggagt
     6541 tgctgctcgc cggcgagatc ggccgcctct actcggtcag ctacgaggag ttcctcgatc
     6601 tcgatcacgg cgccagctac ttccgccgct ggcacgggct caagcaggcg agcggaacgc
     6661 tgctctgcca caaggccagc caccacttcg atcagctcaa ttggtggatc gacgccgacc
     6721 cggtggaggt cacggcggag gggcgcctca acaagtacgg cgccaacggc cgcctgcggc
     6781 acgccaactg ccggctgtgc ccgcacaaga agcagtgcga cctgtactgg gacatgacca
     6841 cgagcaagca gctcatggaa atgtacgccg attgcgaaga ccaggacggc tactaccgcg
     6901 acggctgcct ctaccgccgc gagatcaacg tgcccgacac ctacagcgtg cagatccggt
     6961 actcgaacca ggtgctggtg acctacagcc tgaacgcgac ggtgccttac gaggggcagt
     7021 cgatcgtctt caacggctcg aaggggcgcc tggacgtgcg caactacaac aggcagccgt
     7081 ggcaggtgcc gcacgagagc gagatccggc tgacccgcaa cttcaaggac agcacggtca
     7141 tcacggtcga cccgcagcgc ggcgagttct tcgagcacgg cggggccgac cagcggatca
     7201 agcgaatgat cttcgacacg tcggtacccg accccttgtc acagcgggcc ggcctgaggg
     7261 cgggcatcct cagctcggcc gtcggcatcg ccggctacac cagcatcgag acgggacgga
     7321 aggtgaggat cgacgaggtg gtcaaattgt aggagaaagc agaatgtaga aagcttcctg
     7381 gacgcggtct ggaagctccc tttctccatt ctgcattttt cattttctcg gctccttcac
     7441 ctcctcgccc gtcgtcacct ggaagcggcg gaatttggag taggtggcgc gcccctcgac
     7501 cacggcgcgc ctgatcagcc ccgagcggcc cgagtagccg atatccggct gggagtattt
     7561 ctccctcatc tcggcgggca cccacagccc cagcgcgggg tcggcgcggt agcggacgac
     7621 gatctcggcg cgcgcgtcgc cgcggctcgc gttcatgcgc gtctcgacga ccgcgccggt
     7681 cgccgcgtcg accaggaacg agccgaagac gggcacgtcc tggtgcaggc ggtcgccgat
     7741 cagggtcggc cgggcctgct cggtgtagcg cacccgcacc gcctcgatgc cgcccacgcg
     7801 gtcacgtccc gccagctcgt acgcgaaccg cccacggttg gcggcccgga ggaacgagag
     7861 cggcagcagc ggcacgttcg ccgtgcgctc gacccagccg acgttgtagc gggcgctctc
     7921 ctcgccgagc acctcgagcc tggcccgcgc atcggggctc ggatcgagga acaaccggcg
     7981 cagccgctcc tcgcggtcgc gaaccggctt gccatccacc tcgaacacgt cccgaaacga
     8041 gatccagtcg cccgcctcgt ccggcctcac cagcaggacg tccgacttga ggtgaacccc
     8101 gccggcgctt gccgactgcc ggtaatcctc ctcggccacc agcatcgaat actggcgctc
     8161 gtagccgtcg gcatagtcga acatccgcgc gagcaggacc agacgttcgg cctcgtcgcg
     8221 ggcctcccgc gccgccagct tcctgccggc ggcggcctgc gttgcatccg gctcgccgct
     8281 tgcggcggca ggatcggcgg gcagcgcggc gggacctgcc tccaggaccc ggtctccggg
     8341 agccggcgag gccatcagcc aggaagccgc acgaaccgtg attcccttcc tcgtggtcgc
     8401 aacctcgagg cggtggcgcc cggcccggcc cgggtccgaa gccgtcccga tgccgagcac
     8461 gtagcaggcg cccaggctgc cgatcaccgc atccaccgcg cgcgccggat cccttccaag
     8521 ccgggcgaag gtgcccccgg ttgccgccgc caggcgctcg aggggagcgg tggtcaggga
     8581 gcggcccgcg tcacggccgc ccgtggtgcg ggcgccgaag ccgaagacga cggcacgcga
     8641 cgcggccgcg gcgcgcgccg ccgcgtcgag ctgcgctccg ctggcagccg cgaggccggc
     8701 cgagaacacc gcgacgacct tgcggccggg cacggccgca agcgagtcga gcagcgacgc
     8761 catcgcgccc agcgtgtcgg ggccctccga cacgtcggcc gcgggcgagt tccccgtccg
     8821 gggatcgaac ttgatctggg cttcgggctt cagccccgac atcgtggcct gctcgggcgc
     8881 gtcggtcggg atcggctcgg cctgcgcctg caccgccgcc ggctccaggc gcccggtgac
     8941 gcgggcgagc gcggcccgga cctcggcccg ttcgggcgtg aagctcagga gagcggccgg
     9001 cgccatcggg agggtgacca ccgccacgcg atcgttgagg ccgagccggt ccaggaacag
     9061 ccccgcggcc tgcacgatcg cccgctcatc gccgcgcaga agcgacgatt gatcgacgac
     9121 gacgagcacc gtgcgcgaga gctcggccgc gagttcgatc ccgccggatc cgtctacccg
     9181 cgacgccgcc tcggtcgcgg cgcccggccc ccggctcacc cgccgaaccc acaggacggt
     9241 ccgccgggtg ccgtccaccg tcacgatgaa atcgccggcg cccaggccct cgacgaagcg
     9301 gccgtcgcga cctgctgcgg tgacatcgag cgtcgccggc gctggctcgg caccctggcg
     9361 tgagacggca tccgggccct cggcggcgga cagaagaacg gcggcggcga gcgatgcgaa
     9421 caacgtcgtt gggatcattg acggcgaaag gcgaacaggg ccacgtcggc gtagtcgtag
     9481 gtatcggagg aggcgaagcg ggcgacgtgg aagcggcgcc ggcccatccg ccggcggatc
     9541 gcggcctcgc ctgccgcata ccccgtgttg taggccgtgg cgtagaagcg cagccgctcg
     9601 tcgggaccct tccgcgacac cgccgggtag cgcttctgca tcacgagcat gaacaaccgc
     9661 aggtaccgca cctgccactc gaggtcgtcg aggcggcgca cgcgctgctc ccgcagggcg
     9721 gccgtgtcgc cgtgcccgaa ggcggcgagg ccgaccgcct cgcgctcgcg gcggccgaac
     9781 aggcgcgcgt agtcggactc gacctgctcg gcgaacgagg gcttcatctg gaagcggccc
     9841 accgagaagt cgctgtaggc gcggccgtac tggacgtaca gcaccttgag gccccgaacc
     9901 tggacggcgt cctcgagggc gctgaagcgg accagctcgg ggaagaccac ggccagggcg
     9961 atgcgcgtgt ccatgggcgg caggccaagc gccgaggcca tccaggcgtt ctcacggagg
    10021 aagcgctcgg cgtcggcgta gcgcccgccg aacaccgcgg agta
//
LOCUS       DAOPQX010000105        10063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7607_length_10063_cov_4.606615, whole genome shotgun sequence.
ACCESSION   DAOPQX010000105 DAOPQX010000000
VERSION     DAOPQX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7607_length_10063_cov_4.606615"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKK95_07510"
     CDS             complement(<1..897)
                     /locus_tag="PKK95_07510"
                     /inference="COORDINATES: protein motif:HMM:NF040068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18098.1"
                     /translation="MTSWTTRERMRAILSRETGTIRKPHAGRLRVALAFPNTYNVGMS
                     NLGFQTAYRLFNEADEVSCERVFLPPRSELNASSAQAAPAERLVSLETQTALADFDVL
                     AFSVSFEWDYVNLLTMLRLAGLPLRAAERNRHHPLVVLGGAVTFLNPEPLAPFVDVVC
                     VGEGEVIVPDLVREIARVGDRRELLEALSLRPGIYVPSLVDVSYDAAGRVSAMAPASG
                     SGASVPVRKAVARGPDPGDPPSTVVFTPDTELGSRLLVEVVRGCARGCRFCWAGYNYL
                     PVRAFDAGRLLRIAEAARPHASR"
     gene            complement(1048..2298)
                     /gene="ftsZ"
                     /locus_tag="PKK95_07515"
     CDS             complement(1048..2298)
                     /gene="ftsZ"
                     /locus_tag="PKK95_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOC18099.1"
                     /translation="MANADAVRLEDGLRIQLGEDQRRLGARIKVIGIGGGGSNAVNRM
                     VRAGFPGVEFIVANTDVQALQLSEAPIKVQLGGKLTRGLGAGADPRVGRAAALEDTER
                     LLQVLEGADMVFVTAGMGGGTGTGAAPVVASLAGEMGALTIAVVTKPFAFEGGRRGKQ
                     AQAGLADLRDAVDSVITIPNERLLAVDRSLTLVHAFEAADDVLRQAIQGISDLILVPG
                     LINLDFADVKAVMAGRGVAVMGTGVAEGADRAVEAARRAISSPLLEEASVDGAQGVII
                     NITGGADLGLGEVSDASEIIHSAADPDANIIFGAVVDPTLGSQVKITVIATGFSKQDA
                     RANDAADTPVDLRNYTSWYREHHDPAAAPAPGAAAPIPVRRRSAVVTPAFRQPAEVAE
                     TTAAAGADEHPFDVPAFLRRQREG"
     gene            complement(2339..3574)
                     /gene="ftsA"
                     /locus_tag="PKK95_07520"
     CDS             complement(2339..3574)
                     /gene="ftsA"
                     /locus_tag="PKK95_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOC18100.1"
                     /translation="MARKERYIVGLDVGTSKVAAIVAEPAEHEDLNIVGMGVAESHGI
                     RRGLVVNLEAAVESIKKAIVEAELTAGIEIDNAYLALSGPHVKGFNSRGVVAVAGKNH
                     EIGPEDLSRAIEAAKAVSLPGGRQIILVLPQDFVVDDQDGITAPIGMTGARLEVNVHI
                     VTGGVTATQNLIACVNRAGVQVRGTVLGQLAASDAVLSPDEMDLGVCLLDIGGGTSDL
                     AIFERGSLWHTAVIGVGGDHFTNDIAVGLRMPIPEAERLKRRSGCALSAMVDDEETIE
                     VASVGGRRPRVMGRRILTEVLQPRAEEIFHLVWDEIRSAGYEKSLNSGIVLTGGGAIL
                     EGLPEIAEQIFDLPIRRGAPVKVGGGLADHVNSPGLATAVGLAQRAQRLWKQDVPVNG
                     GGAFSKVAGRIGRIWREFF"
     gene            complement(3646..4488)
                     /locus_tag="PKK95_07525"
     CDS             complement(3646..4488)
                     /locus_tag="PKK95_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOC18101.1"
                     /translation="MPVTAPADRRFLRAQVKPARRRTAWAPRLRAAGRVAAVAALGWL
                     AWGGARMVASSSLLQIEDLHVTGNVRMSRGEVLALVDGLRGRNILSVQLDEWRERLLE
                     CPWVADASLRRSLPSEVEIAIVERTPLAIARIGEDLFLVDERGVLIDEWGPRYEDLDL
                     PILDGLAGKAGPDEDARRAALADRLLRDVRRQPDIGRRISQVDVSDPHNAVVIVDQDT
                     ARVRLGDRDFLARLRAYLEVAPALREQVPEIDYVDLRFGERLYVASLDAARAGSRGAA
                     RGAR"
     gene            complement(4488..5933)
                     /gene="murC"
                     /locus_tag="PKK95_07530"
     CDS             complement(4488..5933)
                     /gene="murC"
                     /locus_tag="PKK95_07530"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOC18102.1"
                     /translation="MLGRTRKIHFVGIGGSGMSGIAELLANLGYSVSGSDAKLSAVTA
                     HLASLGVRVTEGHDARHVGDADVVVYSSAIRPTNPEIVEATRRHVPVIPRAEMLAELM
                     RLKYGIAVAGAHGKTTTTSMIALVLERAGLDPTAVIGGRLSAFGSSARLGKGDFMVAE
                     ADESDRSFLKLSPAIAVITNVDQEHLESYRGFEDLQQAFVAFANKVPFYGAVVACADD
                     PYLRRLLPSVSRRVVTYGLDEPGADYRGIEVAGSGFEWRCQVVRREPADGPGVHAIPR
                     RLGEIVLRVPGRHNLQNALAAVAVGLELDVPFSRIAAALDAFRGAERRFQSHGEAGGV
                     LVIEDYGHHPTEVAAVIGAARSALNRRLLVVFQPHRYSRTRGLLDRFGPALSGADEVV
                     LTDIYAAGEDPIPGVTVDLLAEHVRRAVAAPLHVVRAIADLPREVSRIARPGDVVILL
                     GAGSIGGAAGPIVEALTAAAGGREGGGGGRG"
     gene            complement(5971..7071)
                     /gene="ftsW"
                     /locus_tag="PKK95_07535"
     CDS             complement(5971..7071)
                     /gene="ftsW"
                     /locus_tag="PKK95_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOC18103.1"
                     /translation="MARKLKTDAWLFGGTLVLLAVSMAFVYSASAHRAQLGKNDAEYY
                     LIRQGLWVLLGVALMLVTMRVDYRKWGHPRVIQALVGVTVVGLVAVFFARPVNNAHRW
                     LGIGGVGVQPSEFAKLVVILFAGAVIAARLEARESPLPAFAKVGVLVLVFAALIVSEP
                     DMGSALALTAVGGAVVFTAGIPARWLAALLLTLPVVAYQLAMMEGYRRQRIFAFLAPF
                     EHRREAGFQVVQSLIAVGSGGVLGKGFMQGVQKLFYLPEAHNDFIYAVIAEEKGLVGA
                     SLVLACFVLLAWRGFRVAMHAPDAFGSLVAIGITTMIALQALTNMSVVLSLVPAKGIA
                     LPFVSAGGSSMLVSLGAMGILLNISQQASATT"
     gene            complement(7244..8620)
                     /gene="murD"
                     /locus_tag="PKK95_07540"
     CDS             complement(7244..8620)
                     /gene="murD"
                     /locus_tag="PKK95_07540"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOC18104.1"
                     /translation="MSELEIDGRRIVVVGAARSGIAAAELLARRGGRVTLADLKHELP
                     EATRLEALGIACALGPHDPRLFAAADLIVLSPGVPSRQPPLDRARRAGVPVLGEVEMA
                     SRLLRGRIIAITGTKGKSTTTTLVGEMLRSAGLPARVSGNIGSPLSAEVEGSTPESIH
                     VVEVSSFQLETTSTFHPWIAALLNLSADHLDRHANLREYSAAKARVFANQGPGDHVVV
                     NADDAPAMKLAARKARAPRRMFSVERPLDEGVFVREGWIVEREAGGGEAPLLPVSEVR
                     LIGRHLLSDVAAAAAIARIAGAAGEAVRAAAGSFTGLEHALEPVMTVAGIRFVNDSKA
                     TNVEAARRAIESFDGPLVPIIGGRFKGGDLRDLVPALAGRAKAVVAIGEARPVVHQAL
                     EGAVAIVEAVSMAEAVRAALAASPPGGTVLLAPACSSFDMFRDYADRGRAFKGEVARL
                     AEELDSGS"
     gene            complement(8624..9709)
                     /gene="mraY"
                     /locus_tag="PKK95_07545"
     CDS             complement(8624..9709)
                     /gene="mraY"
                     /locus_tag="PKK95_07545"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOC18105.1"
                     /translation="MLLDLLYPWHGDWPVLNVVRYITFRTAAASLTALAIGLALGPWL
                     IRTLREFQIGQVIRHEGPASHRAKAGTPTMGGLLILAAALVPTLLWADLSNVNVWVAV
                     VTTAAFGAVGFADDYLKIVRHSHHGLRPAYKLAWQVLIAAAVGVVLLELDRRNLYTTR
                     LIFPFFKGLIPDLGWGYAVFAVVVLVGASNAVNLTDGLDGLAISTFTIAAATFTALAY
                     VTGHRVFAEYLLLVRLPEAGELTIFCGSLVGASLAFLWYNAHPAEVFMGDVGSLALGS
                     ALGTVAILVKQELLLVIVGGVFVMEALSVIAQVAAFRLTGRRVFRMAPLHHHFEMIGW
                     QETKVMTRFVILAIVCALFSLTSLKLR"
     gene            complement(9709..>10063)
                     /locus_tag="PKK95_07550"
     CDS             complement(9709..>10063)
                     /locus_tag="PKK95_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOC18106.1"
                     /translation="AALEVVASDRSASRRGAVLGEMLELGEQSTALHERCGRAAAACG
                     LAWLITVGGEPARALGRAAAPGLPPGAVEHAATSDEAAALAVRRVEDGDLVLVKGSRG
                     IATERVVERLRVEFA"
BASE COUNT         1470 a         3801 c         3481 g         1311 t
ORIGIN      
        1 gcgcgaggcg tgggggcgtg cggcctcggc gatgcgcagc agccggccgg cgtcgaaggc
       61 gcgcaccggg aggtagttgt acccggccca gcagaagcgg cacccgcggg cgcagcctcg
      121 cacgacctcg acgagcaggc gcgacccgag ctcggtgtcg ggcgtgaaca cgacggtcga
      181 tggcggatcg ccaggatccg gtcccctggc gaccgccttg cgcaccggca cggaggcgcc
      241 gctccccgag gccggcgcca tcgccgagac gcggcccgcg gcgtcgtagg acacgtccac
      301 gagggagggc acgtagatcc ccgggcggag cgacaacgcc tcgagcagtt cgcggcggtc
      361 gccgacgcgc gcgatctccc gaacgagatc gggcacgatg acctcgccct cgcccacgca
      421 caccacgtcc acgaagggag cgagcggttc ggggttcaga aacgtaacgg cgccgcccag
      481 aaccacgagt gggtggtggc ggttccgctc ggccgcccgc agcggcagcc ccgcgaggcg
      541 gagcatcgtc agcaggttca cgtagtccca ctcgaaggac actgagaagg cgaggacgtc
      601 gaagtcggcg agtgccgtct gggtctcgag gctgacgagg cgctcggccg gggcggcctg
      661 ggcactcgac gcgttcagct cgcttcgtgg cggcaggaac acccgctcgc acgaaacctc
      721 gtcggcctcg ttgaagaggc ggtaggccgt ctggaagccg aggttcgaca tcccgacgtt
      781 gtaggtgttg ggaaatgcaa gggcgacgcg aaggcggccg gcgtggggct tgcggatcgt
      841 gcccgtttcg cgggacagga tcgcccgcat gcgctcgcgc gtcgtccagg atgtcatcgt
      901 gccgggtatg ggcgggaagg ccggggccag cggcgcagcc ggttgtcatc cggctgcgcc
      961 tcaagaggcg ccggtcgcct gtgccgtgga tgttcctagt ctaccacgcg gaccgcgaca
     1021 cccctggggg cgcggcggag tggtgactca gccttcccgc tgccgtcgca ggaacgcggg
     1081 cacgtcgaag gggtgttcgt cggcgccggc ggcggcggtc gtctcggcga cctcggccgg
     1141 ctggcggaac gccggggtga ccaccgccga gcgccgccgc acggggatcg gggccgcggc
     1201 gcccggagcc ggggcggcgg ccgggtcgtg gtgctcccgg taccacgagg tgtagttccg
     1261 caggtccacc ggcgtgtcgg cggcgtcgtt cgcccgcgcg tcctgtttcg agaaccccgt
     1321 ggcgatcacg gtgatcttca cctgggagcc gagcgtggga tccaccaccg cgccgaagat
     1381 gatgttcgcg tcggggtcgg cggccgagtg aatgatctcc gacgcgtcgc tcacttcgcc
     1441 gagcccgagg tcggccccgc cggtgatgtt gatgatcacg ccctgcgcgc cgtcgaccga
     1501 cgcctcctcc agcagcgggc tcgagatcgc gcgtcgcgcg gcctcgaccg cgcggtccgc
     1561 gccctcggcg acgccggtgc ccatcacggc gacgccgcgg cccgccatca cggccttcac
     1621 gtcggcgaag tcgaggttga tgagcccggg gacgaggatc aggtcggaga tcccctggat
     1681 ggcctgccgc agcacgtcgt cggcggcctc gaaggcgtgc acgagggtga gcgaccggtc
     1741 gaccgcgagg agccgctcgt tggggatggt gatgacgctg tcgaccgcgt cgcgcaggtc
     1801 ggcaaggccg gcctgggcct gcttgccgcg gcggccgcct tcgaacgcga acggcttggt
     1861 caccacggcg atggtgagcg cgcccatctc gccggcgagc gaggcgacca cgggcgcggc
     1921 cccggtgccc gtgccgccgc ccatgccggc ggtgacgaac accatgtcgg cgccctcgag
     1981 cacctggagc agccgttcgg tgtcttcgag cgcggcggca cggccgaccc tcggatcggc
     2041 gcccgccccg aggccgcggg tgagcttgcc gccgagctgc accttgatcg gggcttcgct
     2101 cagctgcaat gcctggacgt cggtgttggc gacgatgaac tccacgcccg gaaagcccgc
     2161 acggaccatc cggttgacgg cgttgctgcc gccgccgccg attccgatga ccttgatgcg
     2221 cgcgccgagg cgccgctgat cctcccccag ctggatccgc aggccgtcct cgagccgcac
     2281 cgcgtccgcg ttcgccattc acgcctcccc gtgtcgccct tccgcccccg accgcccctc
     2341 agaagaactc gcgccagatc cgcccgatcc tccccgccac cttcgagaac gcaccgccgc
     2401 cgttcaccgg cacgtcctgc ttccacagcc gctgcgcccg ctgcgcgagg ccgactgccg
     2461 tcgcgagccc cgggctgttc acgtggtccg cgagtccccc gccgaccttc accggcgccc
     2521 cccgccggat gggcaagtcg aagatctgtt ccgcgatctc cggcaggccc tccaggatgg
     2581 cgccgccgcc ggtgaggacg atgcccgagt tcaacgactt ttcgtagccg gcgctgcgga
     2641 tctcgtccca gaccaggtgg aagatctcct cggccctggg ctgcagcacc tcggtgagga
     2701 tccgccgccc catcacgcgc gggcgccggc cgcccacgct ggccacctcg atcgtctcct
     2761 cgtcgtccac catggccgac agcgcgcagc cgcttcgccg cttgaggcgc tcggcctcgg
     2821 ggatcggcat gcgcagcccc acggcgatgt cgttggtgaa gtggtctccg ccgacgccga
     2881 tgacggcggt gtgccagaga ctgccccgtt cgaagatcgc caggtcgctg gtcccgccgc
     2941 cgatgtcgag gaggcagacg ccgaggtcca tttcgtcggg cgagagcacg gcgtcgctgg
     3001 cggccagctg gccgaggacg gtgccgcgca cctgcacgcc ggcccggttc acgcaggcga
     3061 tcaggttctg cgtggcggtg acgccgccgg tgacgatgtg gacgttcacc tcgaggcgcg
     3121 cgccggtcat gccgatgggc gcggtgatcc cgtcctgatc gtccacgacg aagtcctggg
     3181 gaaggacgag gatgatctgc cggccgcccg ggagcgacac cgccttggcc gcctcgatcg
     3241 cgcggctgag atcctcgggc ccgatctcgt ggttcttgcc ggcgacggcg acgacgccgc
     3301 ggctgttgaa ccccttgacg tgcggcccgg agagcgcaag gtaggcgttg tcgatctcga
     3361 tcccggcagt cagctctgcc tcgacgatcg ccttcttgat cgactcgaca gccgcctcga
     3421 ggttcaccac gaggccgcgg cggatgccgt gcgactcggc gacccccatg ccgacgatgt
     3481 tgagatcttc gtgctcggcg ggctcggcca cgatggcggc aaccttcgac gtcccgacgt
     3541 cgagcccgac gatgtaccgc tccttgcgtg ccactccgcc ccctccaccc ggatgccgta
     3601 atcagggccc aacgccgtcg ttcacaccgc gtgacccggc gcccgtcagc gagcgccgcg
     3661 agccgccccc cgcgaaccgg ccctcgcggc gtcgagcgat gcaacgtaga gccgctcgcc
     3721 gaaccggagg tccacgtagt cgatctcggg cacctgctcg cggagcgcag gagccacctc
     3781 gagatacgct cgcagccgcg cgaggaagtc ccggtccccc agccgcacgc gcgccgtgtc
     3841 ctggtccacg atcaccacgg cgttgtgcgg atcggacacg tccacctgcg agatgcgccg
     3901 gccgatgtcg ggctggcgtc tcacatcccg cagcagacgg tccgcgagcg ccgcccgcct
     3961 cgcgtcctcg tcgggccccg ccttgccagc gaggccgtcg aggattggga ggtcgaggtc
     4021 ctcgtagcgc ggcccccact cgtcgatcag cacgccgcgc tcgtccacga ggaacaggtc
     4081 ctccccgatc cgggcgatgg ccagcggtgt ccgctccacg atcgcgatct cgacctccga
     4141 cggcaacgag cggcgcagcg acgcgtcggc cacccacggg cactcgagga gccgctcccg
     4201 ccactcgtcc agttgcaccg acaggatgtt ccggccccgc agtccgtcca ccagcgcgag
     4261 gacctcgccg cgcgacatcc gcacgttgcc cgtcacgtgc aggtcttcga tctgcagcag
     4321 gctcgaggag gccaccatcc gggctccgcc ccaggccagc cacccgaggg ccgccaccgc
     4381 cgcgacccgt ccggccgccc tcaaccgggg cgcccacgcg gtccgccgcc gcgcgggctt
     4441 cacctgcgcc cgcaggaagc gccgatcggc cggggcggtg accggcatca gccgcgcccc
     4501 cctccgccgc cctcccgccc gccggccgcg gcagtgagcg cctcgacgat ggggccggcc
     4561 gcgccgccga tcgagcctgc gccgagcagg atcaccacgt cgccgggccg cgcgattctc
     4621 gacacctccc gcggcaggtc ggcgatcgcc cggacgacgt gcagcggcgc cgccacggcg
     4681 cgtcgcacgt gttcggccag caggtcgacc gtcacgcccg ggatcgggtc ctcgccggcg
     4741 gcgtagatgt cggtgaggac gacctcgtcg gcgcccgaca gggcggggcc gaagcggtcg
     4801 agcagccctc gcgtgcgcga gtagcggtgc ggctggaaca cgacgagcag ccggcggttc
     4861 agcgccgagc gggccgctcc gatcaccgcc gcgacctcgg tcgggtggtg gccgtagtcc
     4921 tcgatcacca gcacgccgcc ggcctcgccg tggctctgga agcgccgctc ggcgccgcgg
     4981 aaggcgtcga gcgcggccgc gatccgcgag aacggaacgt ccagctccag cccgacggcc
     5041 accgccgcga gcgcgttctg gaggttgtgg cgcccgggca cgcggaggac gatttcgccg
     5101 agacgcctcg ggatcgcgtg gacgccgggc ccgtcggcgg gctcacgccg cacgacctgg
     5161 cagcgccact cgaacccgga acccgccacc tcgatgcccc ggtagtcggc gcccggctcg
     5221 tcgaggccgt aggtcaccac gcggcgcgac accgacggga ggagccgccg caggtagggg
     5281 tcgtccgcgc aggccacgac cgcgccgtag aacgggacct tgttcgcgaa ggcgacgaac
     5341 gcctgctgca ggtcctcgaa gccgcggtag ctctcgagat gctcctggtc gacgttggtg
     5401 atgaccgcga tcgcgggcga gagcttgagg aacgaccggt cgctctcgtc ggcctcggcc
     5461 accatgaagt cgcccttccc gagccgggcg ctgctgccga aggcgctgag gcgcccgccg
     5521 atgaccgccg tgggatccag cccggcacgc tcgagcacca gcgcgatcat cgaggtcgtc
     5581 gtcgtcttgc cgtgcgcgcc cgccaccgcg atgccgtact tcagccgcat cagctcggcg
     5641 agcatctcgg cgcgcgggat caccggcacg tggcggcgcg tggcctccac gatctcgggg
     5701 ttggtcggcc ggatggccga cgagtagacg accacgtcgg cgtcaccgac gtgccgggcg
     5761 tcgtggccct cggtcacccg gaccccgagc gaggccaggt gggcggtcac ggccgagagc
     5821 ttcgcatccg agccgctcac cgagtagccc aggttggcca acagctcggc gatgccgctc
     5881 atgccgctgc cgccgatgcc gacgaagtga atcttccggg tgcgtccgag cacaactccc
     5941 ccgccgggcg gcgcgccgcc cggccgactc ctaggtggtc gctgacgcct gctgcgagat
     6001 gttcagcagg atccccatgg cgcccaggct gaccagcatc gacgaaccgc cggcgctgac
     6061 gaacggcagc gcgatcccct tggccggcac gaggctgagc accacgctca tgttcgtaag
     6121 ggcctgcagc gcgatcatcg tggtgatgcc gatcgcgacg agcgacccga acgcgtcggg
     6181 cgcgtgcatc gccacccgga agccgcgcca cgcgaggagc acgaagcagg cgagcacgag
     6241 cgacgcgccc acgagcccct tctcctcggc gatgaccgcg tagatgaagt cgttgtgggc
     6301 ctcgggcagg tagaacagct tctgcacccc ctgcatgaag cccttgccga ggaccccgcc
     6361 cgaccccacc gcgatgagcg actgcaccac ctggaagccg gcctcgcggc ggtgctcgaa
     6421 cggggccagg aacgcgaaga tcctctgccg ccggtacccc tccatcatcg cgagctggta
     6481 cgcgaccacg ggcagcgtca gcagcagcgc cgcgagccac cgcgccggga tcccggcggt
     6541 gaacacgacg gcgcccccca cggccgtcag ggccagggcg ctccccatgt cgggttcgct
     6601 cacgatcagg gcggcgaaga cgagcaccag cacgccaacc ttggcgaaag ccggcagcgg
     6661 cgactcccgc gcctcgaggc gcgcggcgat gacggcgccg gcgaagagga tcaccacgag
     6721 cttcgcgaac tccgagggct gcacgccgac gcccccgatg ccgagccacc ggtgagcgtt
     6781 gttgacgggg cgggcgaaga aaaccgccac cagcccgacg accgtcacgc cgaccagggc
     6841 ctggatcacc cgcgggtggc cccacttgcg gtagtccacc ctcatggtga ccagcatcag
     6901 ggccacgccg agcagcaccc acagcccctg gcggatgagg tagtactccg cgtcgttctt
     6961 ccccagctgc gcgcggtgcg cggaggcgct gtagacgaac gccatgctga cggcgagcag
     7021 cacgagagtg ccgccgaaca gccacgcgtc ggtcttcagc tttcgcgcca tgtcttctcc
     7081 acgccccgtc caaccgagcc ggcaccgggc cggcgccgtc aggtgagcgg cgggcagtca
     7141 tcagtcggcc ggggtcgggc caccggcccc gcaggcgggg ccgcctgcgt tcccagcggc
     7201 cggcgcccga acgccacggg tccgactgac gactgctcgc cgctcaactc cccgaatcca
     7261 gttcctcggc gagccgcgcc acctcgccct tgaaggcgcg cccgcgatcg gcgtagtcac
     7321 ggaacatgtc gaagctcgaa caggcagggg cgagcagcac ggtgcccccc ggcggagacg
     7381 ccgccagcgc cgcgcgcacc gcctcggcca tcgacaccgc ctccacgatc gccacggcgc
     7441 cctcgagtgc ctggtgcacc accggcctcg cctcgccgat ggccaccacc gccttggcgc
     7501 gcccggccag cgccgggacg aggtcgcgca ggtcgcctcc cttgaaacgc ccgccgatga
     7561 tcggcaccag cgggccgtcg aagctctcga tcgcccgccg cgccgcctcg acgttggtcg
     7621 ccttcgagtc gttgacgaag cggatgcccg ccacggtcat caccggttcg agtgcgtgct
     7681 ccagccccgt gaacgatccc gccgcggccc gcaccgcctc gcccgccgcg cccgcaatcc
     7741 gtgcgatcgc cgcggccgcc gcgacgtcgc tgagcaggtg ccggccgatc agcctcacct
     7801 ccgacaccgg gagcaggggg gcctcgccgc ctccggcttc gcgctcgacg atccagccct
     7861 cgcgcacgaa gaccccctcg tcgagcggcc gctccaccga gaacatccgg cgcggcgcgc
     7921 gcgccttgcg cgcggccagc ttcatcgcgg gcgcgtcgtc tgcgttcacc accacgtggt
     7981 cgccgggccc ctggttggcg aagactcggg ccttggcggc cgagtactcc cggaggttgg
     8041 cgtgccggtc gaggtgatcg gccgagaggt tcagcagcgc cgcgatccac gggtggaagg
     8101 tcgaggtcgt ctccagctgg aagctgctga cctcgacgac gtggatcgac tcgggggtcg
     8161 agccctcgac ctccgcgctc aggggggagc cgatgttccc cgagacgcgg gcgggaaggc
     8221 cggccgaccg cagcatctcg ccgaccaggg tcgtggtcgt cgacttcccc ttcgtgccgg
     8281 tgatcgcgat gattcgcccg cgcaggagcc gcgacgccat ctccacctcg cccaggacgg
     8341 gcacccccgc gcgccgcgcg cggtcgaggg gcggctgacg cgagggtact cccgggctca
     8401 ggacgatcag gtcggcggcg gcgaagaggc gcgggtcgtg cggcccgagg gcgcacgcga
     8461 tcccgagcgc ctcgagcctc gtggcctccg gcagctcgtg cttcaggtcg gcgagcgtga
     8521 cccggcctcc gcggcgggcc agcagctcgg cggcggcgat gccgctgcgc gccgccccca
     8581 ccaccacgat gcgccggccg tcgatctcga gctcgctcat cgtctacctg agcttcaacg
     8641 acgtgaggct gaagagcgcg cacacgatcg ccaggatcac gaagcgcgtc atcaccttgg
     8701 tttcctgcca gccgatcatc tcgaagtggt ggtggagcgg cgccatccgg aacacgcggc
     8761 gcccggtcag ccgaaacgcc gccacctgcg cgatcaccga cagcgcctcc atcacgaaca
     8821 caccgcccac gatcaccagg agcagctcct gcttcaccag gatcgccacg gtccccagcg
     8881 ccgagccgag tgcgagcgac ccgacgtcgc ccatgaacac ctcggcgggg tgcgcgttgt
     8941 accagaggaa cgccaggctc gcccccacga gcgatccgca gaagatcgtc agctcgcccg
     9001 cctcgggcag gcgaaccagc aggaggtact ctgcgaacac ccggtggccc gtcacgtacg
     9061 ccaacgccgt gaaggtcgcg gccgcgatcg tgaaggtgct gatcgcgagg ccgtcgagcc
     9121 cgtcggtgag gttgaccgcg ttggacgccc ccaccagcac cacgaccgcg aacaccgcat
     9181 acccccatcc gaggtccggg atcagcccct tgaagaaggg gaagatcaac cgggtggtgt
     9241 acaggttccg gcggtccagc tcgagcagca ccaccccgac cgccgcggca atcagcactt
     9301 gccacgccag cttgtaagcc gggcgcaggc cgtggtgcga gtggcggacg atcttcaggt
     9361 agtcgtctgc gaaccccacc gcgccgaacg ccgccgtcgt cacgaccgcc acccacacgt
     9421 tcacgttcga caggtcggcc cacagcagcg tcggcaccag cgccgccgcc aggatcagca
     9481 ggccccccat ggtgggcgtg cccgccttgg cgcggtggct ggccggcccc tcgtgccgga
     9541 tcacctggcc gatctggaac tcgcgcaggg tccggatcag ccacgggccc agcgccaggc
     9601 caatcgcgag cgcggtcagg ctggccgcgg cggtcctgaa ggtgatgtag cggaccacgt
     9661 tcagaaccgg ccagtcgccg tgccacgggt agagcaggtc gagcagcatc aggcgaactc
     9721 cacccgcagc cgctcgacca cccgctcggt cgcgatcccg cgcgagccct tcacgagcac
     9781 caagtcgccg tcctcgacgc gccggacggc cagggccgcg gcctcgtcgc tggtggccgc
     9841 gtgctcgacg gcccccggcg gcaagcccgg cgccgcggcg cggcccagcg ctcgagcggg
     9901 ctcgccgccc accgtgatca gccaggccag gccgcacgcc gccgcggcgc ggccgcagcg
     9961 ctcgtgcagc gcggtgctct gctcgcccag ctccagcatc tcgcccagca ccgccccgcg
    10021 gcgggacgcc gatcgatccg atgccaccac ctcgagggcg gcc
//
LOCUS       DAOPQX010000106        10038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7633_length_10038_cov_5.049284, whole genome shotgun sequence.
ACCESSION   DAOPQX010000106 DAOPQX010000000
VERSION     DAOPQX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7633_length_10038_cov_5.049284"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PKK95_07555"
     CDS             <1..1309
                     /locus_tag="PKK95_07555"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOC18107.1"
                     /translation="ADEKRIKAVTVCFTDLEGRFHMLDYDKKFLLGSADNLTFDGSSV
                     KGFAEQAESDLRLAVDWTSFYWLPADVFGPGKVLVFGFVQERDGRPYHADMRNRLREF
                     TEQLLQRDGITAHVSNEIEGFLFKGSDVERQYHLTNQFEFISTGGYYHSLPGDALRQF
                     IDGSAEVQRALGFSNEKDHPEVAPSQFEMNYGHTEACIAADQVQLYKLVCRQVAQKMD
                     MTASFLPKPVTRVNGSGMHTNLSLCRGGCNLFWDPNGEDRLSALGWSFVDRVLAAANE
                     LCLIFNSSVNAYRRLDPHFEAPNQIKASAVNRGAMIRIPLANGQSARIELRSIAPDAN
                     PYLAVYALLRTGLEAPLPEDSLNEIRRSRTRFLPDNIHDAIRLFKSSRFLADALGEQV
                     HAKFAEVKQAQADRCPKALGRLIKASEVQFHHEVTNQYLWNRF"
     gene            1920..2579
                     /locus_tag="PKK95_07560"
     CDS             1920..2579
                     /locus_tag="PKK95_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18108.1"
                     /translation="MKTRTRLLPALAALALLVAGTGCVTAYGQPRYGGPYGGGYADVG
                     RIAYDNGYRRGIDRGSDDARSGRAANYRNDRDYRDADWGYDHRYGARGQYRQAFRRGY
                     EAGYRDGFAREARGYGNGRYGGRAVPRDGRYGYPDPRYGGYGYASPAYDKGYRDGFEK
                     GRDDGRDGDRYDPIRHKWYREGDRDYKSRYGSREQYKAAYREAFRQGYEEGYREGRYR
                     R"
     gene            2812..5097
                     /locus_tag="PKK95_07565"
     CDS             2812..5097
                     /locus_tag="PKK95_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18109.1"
                     /translation="MSDTTPAPGFFDGLGDRVIVRDRTAGALEVLRLRTDLTADAAFE
                     PKVRARVQQLAALQHQGLARVRHVGRLAAPDNRLAIVSDLADGLRLSEILQATEQMSL
                     PLHTNAALFLLRQLVSAVACLHGAGPDVSHGALAPERLVVSSTGRLVVVEHVLGSALP
                     HLPHPQPPRVWKDLRVALPPNEEPPFGRRADVLQVGLCGLALVLGRPLRLEEYPLQLR
                     ELLDRATESTVTGDRRPLRAPLRSWLERAMEAAEPAEAPWTIDEARRALDSLLAADGY
                     LATPTGLSTLLESVKRYFAGSDLDPAKAASPEAAPKPPAETGRAHLASPAPPPPPASA
                     QSPQPAPTVAPPAAPVQPPPAASVQPPPAPAPPAPVAPVARLRLVSPPQAPSPLSPAP
                     WSGSLQAPRDAGAVRTEGSLAVATPLAVPPEAAPDDSFAPEAGVSFPPDEPEPEPIVS
                     APALPPPPPRAASGAVPVAAPTLPAGSGAVPVAEPVAEPIAASLARESLAELVHTRPG
                     SGHRASSGSIRPSRSMFGAEPDRGVSSHSGGSSLPLKLGGAAGMLIALAAAGWFIFGS
                     GKAPEKGATAPERRTAVTAPAPKPAAPEPAAPVEPAPARVPGGSEVLPASMAGAAAAA
                     EEAGSLQIESPFALEVFEDGVRLGTTDAPIVLAAGRHQIDLVNRDYAFRGRQAVEVRP
                     GRATRVAAKLPTGLANINAAPWAEVWIDGVRAGETPLGNLSLTIGPHGVVFRHPQLGE
                     QKRTLVVTEGAATRLSVEMQQ"
     gene            5094..6017
                     /locus_tag="PKK95_07570"
     CDS             5094..6017
                     /locus_tag="PKK95_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18110.1"
                     /translation="MTTRITFAAAVVFLVLAGVPLAGAQAGDTLASARSLYGSAAYEE
                     ALAVLDRIKAHEPAADGRQLEQVRAMCLLALGRQGDAERAIEAVISANASFEPDPVTT
                     SPRVQAAFREVRRKVLPGIIQQRYAAAKAAFDSKQYWSAIEQFDAVLTLIGAPDASAG
                     PQEAAMNDLRTLVLGFRDLARSAMPAAPAAPAVAQPAIAEALPPPPPRARPFYGADDK
                     DVIPPVVIDQRLPRWTGPPPAMLASTQRRGAIEVLIDEKGAVESAVLRARTATTYDKA
                     LEDQAMRWKYQPATLKGTPVKYRKMIQFTIE"
     gene            6038..6625
                     /locus_tag="PKK95_07575"
     CDS             6038..6625
                     /locus_tag="PKK95_07575"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC18111.1"
                     /translation="MATSPADHLATLLDQARRGQPAAVEEFVPILYGELRRIAARSLR
                     RERPGQTLQPTALVHEAYLRLLKDEDLSFQNRAHFLAIAARSMREILVEHARARDAQK
                     RGGHRQRVTLDEGVAAGGPRDVELVALDAALERLEQVDPAHARIVELRFFGGLTNEET
                     AEALGVSPATVKRGWAVARAWLFRELSSGQGPAGR"
     gene            complement(6667..7056)
                     /locus_tag="PKK95_07580"
     CDS             complement(6667..7056)
                     /locus_tag="PKK95_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC18112.1"
                     /translation="MMDSAADVLVVDDDPDLAELVRTMLDISGYAARCARNGLEALEA
                     VAQRQPALILLDMMMPVMDGWQCARELHLRYGGRLPIVVLTAAEHARSRTADLVGVAE
                     VLSKPFEMDELLCVVARYAGKARASRM"
     gene            complement(7053..8336)
                     /locus_tag="PKK95_07585"
     CDS             complement(7053..8336)
                     /locus_tag="PKK95_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOC18113.1"
                     /translation="MGESTTWPIVTPATERRVALIVLAVFLMAGAAWILLTDLVLYWV
                     VSDRAVIARLETAKGWMFVALAAGIVYWVTLRSARQLTKTNRTLAAVVKSIGDGVLIL
                     GPNRTIAYANPASVEMLNSTADDLRGMGAEEFSRRFRVSYPDGRLVPPGQFVSQRVFD
                     QPGPIRYKAVLTPPDKPELVISCTAACVRMEIEKAPELVVSVLTDITAAEHLESLRDG
                     LFSAAAHAMKTPVAVIKGASQLISSGVPPALRGSALMIERQCARIDRLVDNLLVLSRI
                     RSGTLQVYPAELELDGLIEEAVAEMTRLSFGHDIRVRIESRPCVRGDRERLRLVVRNA
                     LHSAARASRRGGDLLLRLCRNGDDAEIKVSYHHDLENAAHAAGAPDNDFEDMGTSRHV
                     TAAIVEAHGGSVTEHADSGVTTVGIRLPAIPGDGA"
     gene            8442..9950
                     /locus_tag="PKK95_07590"
     CDS             8442..9950
                     /locus_tag="PKK95_07590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC18114.1"
                     /translation="MPEWSRVTDLFHAALERGPCERAAFLDGACGGDEALRAEVMSLL
                     AAHEGAGGFLDTPAPVAEGLTDPEERTIFEPGRRLGPYEVVREIGRGGMGIVYLATDT
                     RLGRRVAMKVLGPACVGDARRRERLRQEARAAAALSHPGIATVYSLDEIEGHVCLVSE
                     YLEGETLRDEIERGPLPVPRLLETGAQLARALAAAHAAGVVHRDLKPENVRRDESGRP
                     RIVDFGIARLAPGHPGAGHRLTRVGTIVGTPGYMSPEQREGLEVDARSDIYSLGILLY
                     EMATGRHPFEAPALEPAVPRAFASTPPRLSDLRPGLPPGLEAVIRRCLMREVEARSQS
                     ALEVAEDLEAVKAGHLPLRLRLVGDRPLPTLEEPEPARLWWRWHQVATLAVEAAMIVP
                     VARLHALEGTDATLAVLLATIVTAVLNGTIRVHLLFTSAFNRSALAAQLERTRSTLQA
                     SSLAFAASLFAASLAGARRYTVAAGIMAAVAIGWAVVSLLVEPSTRRAAFRE"
BASE COUNT         1432 a         3667 c         3539 g         1400 t
ORIGIN      
        1 ggccgacgag aagcggatca aggccgtcac cgtttgcttc accgacctcg agggccgctt
       61 ccacatgctc gattacgaca agaagttcct gctcggctcg gccgacaacc tcaccttcga
      121 cgggtcgtcg gtgaagggtt tcgccgagca ggccgagtcg gacctccggc tcgcggtgga
      181 ctggacctcg ttctactggc tgccggccga cgtcttcggg ccgggcaagg tgctggtctt
      241 cggcttcgtg caggagcgcg acgggcgccc ctaccacgcc gacatgcgca accgcctccg
      301 cgagttcacc gagcagctgc tgcagcgcga cggcatcacc gcgcacgtct ccaacgagat
      361 cgaggggttc ctcttcaagg ggagcgacgt cgagcggcag tatcacctca ccaaccagtt
      421 cgagttcatc tccaccggcg gctactatca ctcgctgccc ggcgacgccc tgcgccagtt
      481 catcgacggc tcggccgagg tgcagcgcgc gctcggcttc agcaacgaga aggaccaccc
      541 cgaggtcgcg ccctcgcagt tcgagatgaa ctacgggcac accgaggcgt gcatcgcggc
      601 cgaccaggtg cagctctaca agctggtgtg ccggcaggtt gcgcagaaga tggacatgac
      661 cgccagcttc ctccccaagc cggtcacgcg cgtgaacggc agcggcatgc acaccaacct
      721 gtcgctctgc cgcggcgggt gcaacctgtt ctgggacccg aacggcgagg accggctgtc
      781 agctctcgga tggtcgttcg tggaccgggt gctggccgcg gcgaacgagc tgtgcctgat
      841 cttcaactcg agcgtcaacg cgtaccgccg cctcgacccg cacttcgagg cgcccaacca
      901 gatcaaggcc tcggcggtga accgcggcgc gatgatccgg atcccgctcg ccaacgggca
      961 gtcggcgcgc atcgaactgc gctcgatcgc cccggacgcg aacccgtacc tcgccgtgta
     1021 cgcgctgctg cgcaccggcc tcgaggcgcc gctgcccgag gactcgctca acgagatccg
     1081 ccgctcgcgc acccgcttcc tgcccgacaa catccacgac gccatccggc tgttcaagtc
     1141 gtcgcgcttc ctggccgacg cgctcggcga gcaggtgcac gccaagttcg ccgaggtgaa
     1201 gcaggcgcag gccgaccgct gccccaaggc cctcgggcgg ttgatcaagg cgtcggaggt
     1261 gcagttccac cacgaggtga cgaatcagta cctgtggaac aggttctagt ccacggagtc
     1321 acgaagtcac gaggtcccgc cttcgcgctt cgccgctacg gcgggcaggc acgaagtcgc
     1381 gaagtcgagt cgcgaggtcg cgaggtccac gtcctgtggg cctcgcggcc cgatcaatcc
     1441 caggcggcga gcgatcccaa cagcaggccc aggaacgccg cggcccccga gacgatgctc
     1501 atgatgatcg ttcgggcggg tgagccgttg tgctgctccg cgacccgcag gaccgagatg
     1561 cgtcaacgtc gcgttgaacc agccggcggc cgcgctcacg tctgcgcgcg gcaggccagg
     1621 tgccgcgtcc ggcggaccgg aaggcgacgg ggtttgagcg gagaggggcg agaacgcgcc
     1681 ggccgcgagg aggcacgcga gcacgatctc agatgcgaca acccacggcg aatgggctca
     1741 aggcgggcat ccgcctacgg cgccgcccgg ggacaccggc cccgccccag ctgtccgcca
     1801 ccagcgacgc atccccggcg gcatcccggg aatcggccat tctccgagcg ttttcgagcg
     1861 tttccacgtg gcccttcctt tgctgtcctc atctgcagga gttgaccacg ggggacgcca
     1921 tgaagactcg aactcgcctg ctgcccgcgc tcgccgccct cgcgctgctc gtggccggca
     1981 ccggctgcgt caccgcctac ggccagccgc gctacggcgg cccctacggc ggtggctacg
     2041 ccgacgtcgg ccgcatcgcg tacgacaacg gctaccgccg ggggatcgac cgaggctcgg
     2101 acgacgcgcg ctcggggcgc gccgcgaact accgcaacga ccgcgactac cgcgacgccg
     2161 actggggcta cgaccaccgc tacggcgcgc ggggccagta ccgccaggcg ttccgccgcg
     2221 gctacgaggc cggctaccgc gacggcttcg cgcgcgaggc ccgcggctac ggcaacggcc
     2281 gctacggcgg gagggcggtg ccgcgcgacg gccgctacgg ctatcccgac ccgcgctacg
     2341 gtggctacgg ctacgcgtcg cccgcctacg acaaggggta ccgcgacggg ttcgagaagg
     2401 gccgcgacga tggccgcgat ggcgaccggt acgacccgat ccggcacaag tggtaccgcg
     2461 agggcgaccg cgactacaag tcgcgctacg ggtcgcgcga gcagtacaag gccgcctacc
     2521 gcgaggcatt caggcagggc tacgaggagg gctaccgcga gggaaggtac cggcggtagg
     2581 actcactgac tggccgggcg gtcgtgaatc ggccgcccgg tcaattacgt tttggcgcaa
     2641 agctcaacgg tttcgtaatc ctgcccgccg cccgcgcccg tcccagacgc gcggggatcg
     2701 ctcaaaacat tcagcaaact aaccattttc ggacccaacg gaagaggtgc caatcttgcc
     2761 ttcccagctg aggatccgta tcgccgccag actgctggga gcgtcagcac catgtccgat
     2821 accacccccg ccccgggatt cttcgacggg ctcggcgacc gcgtcatcgt ccgcgaccgc
     2881 acggcaggcg ccctcgaggt gctgcgcctg cggaccgacc tgactgccga tgccgccttc
     2941 gagccgaagg tgcgtgcccg cgttcagcag ctcgccgcac tgcagcacca ggggctcgcg
     3001 cgcgtccggc acgtcggccg ccttgccgcg cccgacaacc gcctggcgat cgtctccgac
     3061 ctggccgacg gcctccggct ctccgagatc ctgcaggcga ccgagcagat gtcgctcccc
     3121 ctgcacacca acgcggcgct cttcctcctg cggcagctcg tgtccgccgt ggcgtgcctc
     3181 catggcgccg gccccgacgt ctcgcatggc gccctcgcgc ccgagcggct ggtggtttcg
     3241 tccaccggac gtctcgtcgt ggtcgagcac gtgctcggca gcgccctgcc gcacctgccg
     3301 cacccgcagc cgcctcgcgt gtggaaggat ctccgcgttg cgcttccccc caacgaggag
     3361 ccgccgttcg gccggcgcgc cgacgtcctg caagtggggc tgtgcggcct cgcgctcgtc
     3421 ctcggccggc cgctgcgcct cgaggagtac ccgctgcagc tgcgcgagct gctcgaccgc
     3481 gcgaccgaga gcacggtcac cggcgaccgc cgcccgctgc gcgcgccgct gcggtcatgg
     3541 ctggagcggg cgatggaggc ggccgaaccg gccgaggcgc cctggaccat cgacgaggct
     3601 cgacgtgcgc tcgactcgct gttggccgcc gacggctacc tcgccacgcc aacgggcctc
     3661 tccacgctcc tcgagagcgt gaaacgctac ttcgcgggct ccgacctcga tccggccaag
     3721 gccgcctcgc ccgaggccgc gcccaagccg cccgccgaaa cgggccgggc acacctggcc
     3781 tcgcccgcgc cgccgcctcc cccagcatcg gcacagtcgc cgcaacccgc gcccactgtg
     3841 gcgccgccgg ccgcgccggt acaaccgccg ccggccgcgt cagtacagcc gccgccggcg
     3901 cccgcgccgc ccgcgccggt tgcgccggtt gcgcggcttc gcctcgtgtc gccgccgcag
     3961 gccccctcgc cgctttcgcc cgcgccatgg tccgggtcgc tgcaggcccc gcgcgacgcc
     4021 ggcgccgtgc gcaccgaggg ctcgctcgcg gtcgccaccc cgctggccgt cccgcccgag
     4081 gcggcgcccg acgacagctt cgcgccggag gccggcgtgt cgtttccgcc cgacgagccg
     4141 gagcccgagc cgatcgtctc cgcgcccgcg ctgccgccgc cgcccccgcg ggcggcgagc
     4201 ggcgcggttc ccgtcgccgc ccccacactc ccggcgggca gcggcgcggt tcccgtggca
     4261 gaaccggtgg ctgaaccgat cgccgcgagc ctggcgcgcg agagcctggc agagctggtg
     4321 cacacgcggc cgggttccgg gcaccgtgct tccagcggat ccatccggcc gtcgcggtcg
     4381 atgttcggtg ccgagcccga tcgcggcgtg tcgagtcatt cgggaggatc gagcctcccg
     4441 ctgaagctgg ggggggcggc cggcatgctg atcgccctgg ccgcagcagg ctggttcatc
     4501 ttcggttcgg ggaaggcgcc ggagaagggc gcgacggcgc cggagcgccg tacggccgtg
     4561 accgcgccgg ccccgaagcc cgcggccccc gagccggcgg cccccgtcga gccggcaccc
     4621 gccagggtgc cggggggaag cgaggtgctc ccggcgagca tggccggtgc ggccgctgcc
     4681 gccgaggagg ccggcagcct gcagatcgag agcccgtttg cgctcgaggt gttcgaggac
     4741 ggcgtgcggc tcggcaccac cgacgcgccc atcgtcctgg ccgccggccg ccaccagatc
     4801 gacctggtga accgcgacta cgccttccgg ggacggcagg cggtggaggt tcgcccgggg
     4861 cgcgcgacac gcgtggcggc aaagctgccg accggcctgg cgaacatcaa cgcggcgccc
     4921 tgggccgagg tgtggattga cggggtgcgg gccggcgaga cgccgctcgg caacctgtcg
     4981 ctgaccatcg ggccgcacgg cgtcgtgttc cggcacccgc agttgggcga gcagaagagg
     5041 acgctcgtgg tgacggaagg ggccgccacc aggctgagcg tggagatgca gcaatgacca
     5101 cgcgcatcac tttcgccgcg gccgtcgtct tcctggtgct ggccggcgtt cccctggcgg
     5161 gcgcacaggc cggcgacacg ctcgcgtcgg cgaggagcct ctacgggtcg gcggcgtacg
     5221 aggaagcgct ggcggtgctc gatcggatca aggcgcacga accggcggcc gacggccggc
     5281 agctggaaca ggtcagggcc atgtgcctgc tcgcgctcgg gcggcagggc gacgccgagc
     5341 gcgcgatcga ggcggtcatc agcgccaacg cctccttcga gcccgacccc gtcaccacgt
     5401 cgccgcgcgt gcaggccgcc ttccgcgagg tgcgccgcaa ggtgctgccg ggaatcatcc
     5461 agcagcggta cgccgcagcc aaggcggcgt tcgacagcaa gcagtactgg tcggcgatcg
     5521 agcagttcga cgccgtgctg acgctgatcg gagcgcccga cgcgtcggcc ggcccgcagg
     5581 aagcggcgat gaacgacctg cggacgctgg tgctcggctt ccgcgacctg gcgcgatcgg
     5641 cgatgccggc ggccccggcg gcgccggcgg tggcgcagcc ggcgatcgcc gaggcgctgc
     5701 cgccgccccc gccccgtgcg cgcccgttct acggcgccga cgacaaggac gtcatccctc
     5761 ccgtagtgat cgatcagcgc ctcccccggt ggaccgggcc gccgcctgcg atgctggcct
     5821 ccacccagcg ccgcggcgcc atcgaggtgc tgatcgacga gaagggcgcg gtcgagtcgg
     5881 ccgtgctgcg tgcgaggacc gccaccacct acgacaaggc gctcgaagac caggcgatgc
     5941 gctggaagta ccagccggcg accctgaagg gcacgccggt caagtaccgg aagatgatcc
     6001 aattcacgat cgagtaggga atcgctatac tcccccggtg gccacctcgc ctgctgacca
     6061 cctggccacg ctgctcgatc aagcccggcg ggggcagccg gcggccgtcg aggagttcgt
     6121 cccgatcctc tacggcgagc tccgccggat tgccgcacgc tcccttcgcc gcgagcggcc
     6181 cgggcagacc ctgcagccga ccgcgctcgt gcacgaggcc tacctgcggc tgctgaagga
     6241 cgaagacctt tccttccaga accgcgcaca cttcctcgcc atcgccgccc gctcgatgcg
     6301 ggagattctc gtcgagcacg cccgggcgcg cgacgcgcag aagcgaggcg ggcaccgcca
     6361 gcgggtcacg ctcgacgagg gggtggccgc cggcgggccg cgcgacgtcg agctggtggc
     6421 gctcgacgcg gcactcgagc ggctcgagca agtggacccg gcccacgccc ggatcgtcga
     6481 gctcaggttc ttcggcgggc tgaccaacga ggagacggcc gaggcgctcg gcgtgtcgcc
     6541 ggccacggtg aagcgcgggt gggcggtggc gcgcgcgtgg ctcttcaggg aactcagttc
     6601 agggcagggg ccggcaggcc gctgacgaac gcagcccgaa agctctcctc ggccgggggc
     6661 gcgtcttcac attctggacg cacgcgcctt cccggcgtaa cgtgccacca cgcacaacag
     6721 ttcgtccatc tcgaacggct tggagaggac ctccgcgacg ccgacgaggt cggcggtgcg
     6781 cgagcgggcg tgttccgcgg cggtcaatac gacgattggc agccggcctc cgtatcgcag
     6841 gtgcagctcg cgggcgcact gccacccgtc catcaccggc atcatcatgt cgagcaggat
     6901 cagcgcgggc tgcctctgtg caacggcctc cagggcttcc aggccgttgc gggcgcacct
     6961 ggccgcgtag cccgagatgt cgagcatggt ccgcaccagc tcggccaggt cggggtcatc
     7021 gtcgacgacc agcacgtccg ccgctgaatc catcatgcgc cgtctccggg gatggccggc
     7081 agccggattc ccaccgtcgt cactccgcta tcggcgtgct cggtgacgct cccgccgtgc
     7141 gcctcgacga tcgccgccgt gacgtgacgg ctggtcccca tgtcctcgaa gtcgttgtcc
     7201 ggcgcgccgg cggcgtgggc ggcgttctcc agatcgtggt ggtaggacac cttgatctcg
     7261 gcatcgtccc cgttgcggca caggcgaagc agcaggtcgc cgccgcgccg cgaggcgcgg
     7321 gcggcggagt gaagggcgtt gcggacgacg agccgaagcc gctcgcggtc gccgcgcacg
     7381 cagggacggc tttcgatgcg gacacggatg tcgtgtccga acgagagcct ggtcatctcg
     7441 gcgacggcct cctcgatcag gccatcgagt tcgagttcgg cggggtagac ctggagcgtt
     7501 cccgaccgga tgcgcgagag cacgaggagg ttgtccacga ggcggtcgat gcgcgcgcac
     7561 tggcgctcga tcatcagggc cgagccgcgg agcgcaggcg ggaccccgct cgatatcagc
     7621 tgcgacgccc ccttgatgac ggccacgggc gtcttcatcg cgtgcgcggc cgccgagaac
     7681 aggccgtcgc ggaggctctc gaggtgttcc gcggcggtga tgtcggtcag cacgctcacc
     7741 accagctcgg gcgccttctc gatctccatc cggacgcagg cggccgtgca ggagatgacc
     7801 agctcgggct tgtcgggcgg agtgaggacc gccttgtaac ggatgggccc gggttggtcg
     7861 aacacgcgct gcgagacgaa ctggccgggc ggaacgaggc ggccgtcggg gtacgacacc
     7921 ctgaagcgcc gcgagaactc ctcggcgccc atcccgcgca gatcgtcagc cgtcgagttc
     7981 agcatctcga cagaggcggg attcgcgtag gcgatcgtcc ggttgggccc caggatcagc
     8041 actccgtcac caatactctt tacgacggct gcaagggtcc tgttggtctt ggtcagttgc
     8101 cgggcgctgc gcagggtcac ccagtagaca atgccggcgg ccagcgcgac gaacatccac
     8161 cccttcgccg tttcgagacg ggcaatgacc gctcgatccg agacgaccca gtacagcacc
     8221 aggtcggtca ggaggatcca cgccgccccg gccatgagga acaccgcgag cacgatgagc
     8281 gcgacgcgcc gctcggtggc cggtgtgacg atgggccagg tggtcgattc gcccattcgg
     8341 gcacctccct tgggggaacg tgggccgacc gtgcaagcgc cgtgctacat ccttcttccg
     8401 gggcgttaca ctcttgccgt ggcccgctgg cggccatacc catgcccgag tggtcccgcg
     8461 tcaccgacct cttccatgcc gcgctcgagc gtggcccctg cgagcgcgct gcgttcctcg
     8521 acggtgcctg cggcggcgac gaggcgctgc gcgcggaggt gatgtcgctg ctcgcggccc
     8581 acgagggggc cggcgggttc ctcgacacgc ccgcccccgt cgccgagggg ctcacggatc
     8641 ccgaggagcg cacgatcttc gagccggggc ggcggctcgg gccgtacgaa gtcgtgcgcg
     8701 agatcgggcg cggcgggatg gggatcgtct acctagccac cgacacgcgg cttggccgcc
     8761 gggtggcgat gaaggtgctg ggacctgcct gcgtgggcga cgcgcggcgc cgcgagcggc
     8821 tgcgccagga ggcgcgggcg gcggcggctc tctcgcaccc cggcatcgcg acggtctact
     8881 cgctcgacga gatcgaggga cacgtctgtc tcgtctcgga atacctggag ggagagacgc
     8941 tgcgcgacga gatcgagcgc ggccccctgc cggtgccgcg gctgctggag accggcgcgc
     9001 agctggcgcg cgcgcttgcg gcggcgcacg cggccggggt cgtgcaccgc gacctcaagc
     9061 ccgagaacgt ccggcgcgac gagtcggggc gcccgcgcat cgtggacttc gggatcgcgc
     9121 gcctcgcgcc gggtcacccg ggcgccgggc atcgtctcac ccgcgttggc accatcgtcg
     9181 gcacgcccgg ctacatgtcg ccagagcagc gcgagggact ggaggtggat gcgcgcagcg
     9241 acatctactc gctgggcatc ctgctctacg agatggccac gggacggcat cccttcgagg
     9301 cgccggccct cgagccggcg gtgcctcgcg ccttcgcttc caccccgccg cgcctttctg
     9361 atctgcgccc gggcctgccg ccgggcctcg aggccgtcat ccggcgatgc ctgatgcgcg
     9421 aggtggaggc gcgctcgcag agtgcgctcg aggtggccga ggatctggag gcggtgaagg
     9481 cggggcacct gccgcttcgc ctgcgtctgg tcggtgatag gccgcttccg acgctcgagg
     9541 agcccgagcc ggcgcggctc tggtggcgct ggcaccaagt ggcgaccctc gcggtcgagg
     9601 cggcgatgat cgtcccggtg gcgcggctgc acgcgctcga ggggaccgac gcgacgctcg
     9661 ccgtgctgct ggccacgatc gtgaccgcgg tgctcaacgg caccatccgg gttcacctgc
     9721 tcttcacatc ggcgttcaac cgatcggccc tggccgcgca gctcgagcgc acgcgctcga
     9781 cgcttcaggc ctcgagcctg gcgttcgccg cctcgctgtt cgccgcgtcc ctcgccggcg
     9841 cgcgccgcta caccgtggcc gcggggatca tggccgccgt ggcgattggc tgggccgtgg
     9901 tgtcgctgct ggtcgaaccc tccacccgcc gggcggcgtt cagggaatag gggcgaccct
     9961 tgtggtcgcc cgattcggct cgcggctccc gccttcgcca aacgctcgct acgctcgcgg
    10021 ctccggcggg caagcagg
//
LOCUS       DAOPQX010000107        10035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7635_length_10035_cov_4.314830, whole genome shotgun sequence.
ACCESSION   DAOPQX010000107 DAOPQX010000000
VERSION     DAOPQX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7635_length_10035_cov_4.314830"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKK95_07595"
     CDS             complement(<1..475)
                     /locus_tag="PKK95_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18115.1"
                     /translation="MATVAAPVLGTRPHRALGLLWRQLEVVNKELWIVLSVFVIAAIL
                     NFATSQRMVLALYTLPTLLSAYCYGRRHATLTALASALVVGGVLYANPALMGMSPAAL
                     SPEAAMVDVGLWACVLMVTAYATGTLYEHKAAQCEELRQTYYGLLEVLRHFISNDK"
     gene            567..2180
                     /locus_tag="PKK95_07600"
     CDS             567..2180
                     /locus_tag="PKK95_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOC18116.1"
                     /translation="MPPATGSPAGRREFTTVSGRPVRVLYTPADVAHLDYARDLGDPG
                     TFPYTRGIHATGYRGKLWTMRQFAGFGTPGETNARYKELLANGGTGLSVAFDLPTLMG
                     RDPDHPLSLGEVGKCGVSIVSLADMEALFDGIDLSSITTSMTINSPAPVLFAMYLAVA
                     ERQGADWRQLSGTIQNDILKEFIAQKEFIYPPRPSMRLVTDVFRFCRDEVPRWNTISV
                     SGYHIREAGATAVQELAFTLRDGVEYVQYGVDAGLGVDEFVPRLSFFFNAHNDFFEEI
                     AKYRAARRIWARVMRERFGARDERSLKLRFHSQTAGVSLTAQQPYNNVVRTALQALAA
                     VLGGTQSLHTNSLDEALALPTREAVTLALRTQQIIAHESGVANVVDPLGGSYFVEHLT
                     GEMEGEALDIMGVIDAMGGMVAAIERGFPQREIAESAYRYQQAVERREKIIVGVNEFV
                     ADEEEPVPILYIDEAAADRQLAGLERLRRNRDGDRVRRALDGLREAAAGTANTMPPIL
                     EAVRAYASIGEICDALREVWGEYEEVPAI"
     gene            2191..2598
                     /locus_tag="PKK95_07605"
     CDS             2191..2598
                     /locus_tag="PKK95_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOC18117.1"
                     /translation="MPRIRVIIAKPGLDGHDRGAKIIARGLRDAGMEVIYTGLRQTPE
                     QIVAAALQEDADVIGLSILSGAHTRICPRLMELLREHGLDDVLVVLGGIIPDPDVPAM
                     QALGIRGIFRPGASMAEIVDFIERNVRAREEQG"
     gene            2752..3729
                     /locus_tag="PKK95_07610"
     CDS             2752..3729
                     /locus_tag="PKK95_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC18118.1"
                     /translation="MPHKLLLADDSVTIQRVIELTFADEDVRVKAVSTGQQAIDEVSS
                     DPPDIVLADVGMPERDGYEVAAFVKGRPELAHIPVLLLTGAFEPVDDARAAAAGCDGV
                     LAKPFEPQILISRVKELLPKAGAPARAGVHAAAAEAAPAPSFDAVPPAPPGEEEAALD
                     MHALLDSQPGRSPEADVSLDDYFDRLDQAFASLAGGAGRAGSVAPEGWGGPAEAPATA
                     LEPAGPPAVPAAHAPAPPQALRAVPAADLFSSVLEAEAAGAPAAPSPLAVFVPPPPPV
                     TDELVERIVRLVIERMAERDVREALADTVSRVAERLVREEIERLKALPR"
     gene            3818..6463
                     /locus_tag="PKK95_07615"
     CDS             3818..6463
                     /locus_tag="PKK95_07615"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOC18119.1"
                     /translation="MKTPLPKGFDHADVDPKWYAFWESIGAFRADPSSDKPRFSMVIP
                     PPNVTGSLHMGHALNHTLPDIVARWKRMQGYDVLWLPGTDHAGIATQNVVEKQLALEG
                     KTRHDLGREAFEARVWEWVRQSHGTITGQMRKLGESVDWSRERFTLDEMLSRAVRRVF
                     VSLYQDGLIYRGTYIVNWCPRCRTALSDLEVVHQPKAAQLWYVRYPGTKGGRGVTVAT
                     TRPETMLGDTAIAVNPGDERYRDLVGGHVILPIIGRELPVIADEFVDPAFGTGAVKVT
                     PAHDPNDFQMGQRHGLPQVAVIDEDARITAAGGPYAGQDRFAAREGIVAQLEREGLLE
                     KIEPHEHAVGTCQRCGTVVEPLVSTQWFVKVAPLAKEAIRVVEQGKIRFFPETWTKTY
                     YDWMYNIHDWCISRQLWWGHRIPAWYCEACGEMIVSEQAPERCPCGGTLRQDPDVLDT
                     WFSSQLWPFSTMGWPDRTGDLARYYPTDLMLTAFDIIFFWVARMIMAGVRFAGDIPFR
                     EVYITGLVRDEGGRKMSKSKGNVVDPLEVMDEIGADALRFTLTAMAAPGMDIPLSEGR
                     MQGYRQFINKIWNASRFVLMNAGELEERPEIPAGAPLALIHRWILHRLNVVTGEVQES
                     LETFRFDVAADRLYHFFWDEYADWYIEMVKPHLQAGGDEGRIAQAVLLEVHDRTLRLL
                     HPIIPFVTEELWQHLPRRAGDGPTVTLAAWPKVRPEWVDEAADAEVGLLQGVVTTIRT
                     ARAEGKVKPSAKVGVTLEGAGEEQVRILRAQQRYLTTLAGLSSFEFGAEAPGGEDLVT
                     RVLGDLRVHIQLPHRDRGAEVEKLRKQLADKLREVAGVDAKLANEEFVAKAPARVVEG
                     ARAQRDKLLVEQRRIEETLRELGEP"
     gene            6460..7305
                     /locus_tag="PKK95_07620"
     CDS             6460..7305
                     /locus_tag="PKK95_07620"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOC18120.1"
                     /translation="MSPGETPAAVPPDLIAALDARRARLGGFAAFLRYFKSVGSTNDV
                     AARLADAGAPHGAAVIAVAQERGRGRMGRGWFSPPGSGLYVSVVLRPAALGAGFGSGA
                     SGASVASSVTLSAGVAVAEAIREVSGLRVEIKWPNDLVVGRRKLCGILAEASAAASGL
                     QHVVLGFGINLRAAGYPPDLAGRATSIEEELGRPVDEQVLLAEALARLSETISGLPVR
                     GLEAVLRRWRELSPSASGARVEVLAGAGRWEIATTAGIDDDGALLVTRAGSIHRVIAG
                     EVRWL"
     gene            7315..8100
                     /locus_tag="PKK95_07625"
     CDS             7315..8100
                     /locus_tag="PKK95_07625"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOC18121.1"
                     /translation="MLLALDIGNTNIVVGVFDGNDLLHSWRLSTLRQRTADEMGIWLV
                     QLFRHGGLEVGGVDGVVMASVVPTLTRPTLEMAKRYFGHAPLVVDGEAKTGMPVLYEA
                     PAEVGADRIVNGVAAFERHGTADGRPRALVVVDFGTATTFDAITSRGEYLGGVICPGV
                     TISADALFERAARLPRVDVRKPAAVIGRTTVGSMQSGLFYGYVGLVEGIVERMKRELG
                     APAGCVATGGLAEMIAPETPAIEAVDRDLTLHGLRIIWDRNRG"
     gene            8116..8829
                     /locus_tag="PKK95_07630"
     CDS             8116..8829
                     /locus_tag="PKK95_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18122.1"
                     /translation="MDPDQYCREIESYLCRRNAGHLVRLVGPAFEMVAGWARTGIPIR
                     VACAGIDRYLDRHDAKAGGAPAARRRPVRAEFCEADVLDAFDQWRRAVGLGTAGSAAP
                     EAAAGGGGHQSLRAHLDRVATRLTDRLADGSVAPILEAHLERLLDELSRMREGARTVR
                     GEARQQVIARLEELDRALLEAVQAAAGAELLESATAEAEADLGDFRTRMAAAEYARAR
                     DAAVRALLRDRCRLPVIAY"
     gene            9010..>10035
                     /locus_tag="PKK95_07635"
     CDS             9010..>10035
                     /locus_tag="PKK95_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18123.1"
                     /translation="MLARHDGLVVLVSAAIPGEQVRALVQRTSAGVAYAVTVEVVKAS
                     PDRRPAATDWACGGNVYAHIAYPRQLSLKAEIVLDALARIGHITHQAPIEVSPSPERG
                     YRMRARFHLRGSHIGFFREGTHHLCDPASTGQLLPETDRVLAGIGEILRTGRLQGVTS
                     IELAENVPATERALHLELERPVPPAGLELLARIPTVVGVSASFGGASPSAGRHPGQGQ
                     GWRRTMTAGGAACVLDDLALPDTPPFRLQHNAAAFFQGNRYLLAPLAARVCSLVPRGP
                     VIDLYAGVGLFAAALAASGWERVVAVESDRAAAADLRANAAPFGAALEPVEMPVEDYL
                     AGHQSDGA"
BASE COUNT         1438 a         3510 c         3668 g         1419 t
ORIGIN      
        1 acttgtcgtt ggagatgaag tggcggagca cctcgaggag gccgtagtag gtctgccgca
       61 gctcctcgca ctgcgcggcc ttgtgttcgt agagcgtgcc ggtcgcgtag gccgtcacca
      121 tcagcacgca ggcccacagc cccacgtcca ccatcgccgc ctcgggcgac agcgcggccg
      181 ggctcatgcc catcagcgcc gggttggcgt acagcacgcc gccgaccacc agcgcgctgg
      241 caagcgcggt cagcgtggcg tggcggcggc cgtagcagta tgcagagaga agggtcggca
      301 gcgtgtagag cgcgagcacc atccgctgcg aggtcgcgaa gttcaggatc gccgcgatca
      361 cgaagaccga caggacgatc cacagctcct tgttgacgac ttcgagctgg cgccacagca
      421 agccgagcgc ccggtgaggg cgtgtcccga ggaccggcgc ggcaaccgtt gccatttgaa
      481 acacctcgcg cgcgctggcc gttcgctggc caacgattgt ttcggcgccg gacggcccgg
      541 cctttacgcg cctgtataat tgcgctatgc ccccagcgac cggttccccc gccggccggc
      601 gcgagttcac gaccgtctcg ggccgcccgg tgcgcgtcct ctacacgccg gccgacgtcg
      661 cccacctcga ctacgcccgc gacctgggcg accccggcac cttcccgtat acccggggca
      721 tccacgccac cggctaccgc ggcaagctct ggacgatgcg ccagttcgcg gggttcggca
      781 cgcccggcga gaccaacgcc cgctacaagg agctgctggc gaacggcggc accgggctca
      841 gcgtggcgtt cgacctgccg acactgatgg ggcgcgaccc cgaccacccg ctctcgctcg
      901 gcgaggtggg gaagtgcggc gtgagcatcg tctcgctcgc ggacatggag gcgctcttcg
      961 acgggatcga tctgtcgtcg atcaccacgt cgatgacgat caactcgccg gcgcctgtgc
     1021 tgttcgcgat gtacctggcg gtggccgagc ggcagggtgc cgactggcgg cagctctccg
     1081 gcacgatcca gaacgacatc ctcaaggagt tcatcgctca gaaggagttc atctacccgc
     1141 cccgtccctc gatgcgcctg gtcaccgacg tcttccgctt ctgccgcgac gaggtgccgc
     1201 gctggaacac catctcggtc agcggctacc acatccggga ggcgggcgcg acggcggtcc
     1261 aggagctggc cttcacgctg cgcgacggcg tcgagtacgt gcagtacggc gtggacgccg
     1321 ggctgggggt ggacgagttc gtgccgcggc tctccttctt cttcaacgcg cacaacgact
     1381 tcttcgagga gattgcgaag taccgggcgg cccggcgcat ctgggcccgg gtgatgcgcg
     1441 agcggttcgg cgcgcgcgac gagcggtcgc tcaagctgcg cttccactcg cagacggcgg
     1501 gagtgtcgct caccgcgcag cagccgtaca acaacgtcgt gcggacggcg ctgcaggcgc
     1561 tcgcggccgt cctcggcggc acccagtcgc tgcacaccaa ctcgctcgac gaggcgctcg
     1621 cccttccgac ccgggaggcc gtcaccctcg cgctccggac acagcagatc atcgcgcacg
     1681 agagcggggt ggccaacgtc gtcgaccccc tcggcgggtc gtacttcgtc gagcacctca
     1741 ccggcgagat ggagggcgag gccctcgaca tcatgggcgt catcgacgcg atgggcggaa
     1801 tggtggcggc aatcgagcgc gggttccccc agcgcgagat cgccgagagc gcctaccgct
     1861 accagcaggc cgtcgagcgg cgcgagaaaa tcatcgtcgg cgtcaacgag ttcgtggcgg
     1921 acgaagagga gcccgtcccg atcctctaca tcgacgaggc cgccgccgac cggcagcttg
     1981 ccgggctcga gcgtctccgc cgcaatcgcg acggcgaccg cgtgcgccgc gcgctcgacg
     2041 gcttgcgcga ggcggccgcc gggacggcca acacgatgcc cccgatcctc gaggccgtcc
     2101 gggcctacgc ctccatcggc gagatctgcg acgcgctgcg cgaggtgtgg ggcgagtacg
     2161 aggaagtgcc ggccatctaa tcgaccacct atgccgcgaa tccgcgtcat catcgccaag
     2221 ccgggcctcg acggccacga tcggggcgcc aagatcattg cccgcggcct gcgcgacgcc
     2281 ggcatggagg tgatctacac cggcctccgc cagacccccg agcagatcgt cgcggccgcc
     2341 ctgcaggagg acgccgacgt catcggcctt tcgatcctct ccggcgcgca cacccggatc
     2401 tgcccgcgcc tgatggaact cctgcgcgaa cacgggctcg acgacgtgct ggtcgttctc
     2461 gggggcatca tccccgaccc ggacgtgccg gcaatgcagg cgctcgggat ccgtggcata
     2521 ttccggccgg gggcgtcgat ggccgagatc gtcgacttca tcgagcgcaa cgtccgcgcg
     2581 agggaagagc agggctgaag ccgcgccggc tgcttactcc gcgaggcgcg gggccgaagc
     2641 ctcggtgcag gggcgcgggg cgctgaagac atggggcgaa ccggccgata acccggctgt
     2701 cgcctcccgc ggcggttgtg gaggcgctcg tgccggaggg tcgagggccg aatgccgcac
     2761 aaactcctgc tcgccgacga cagcgtcacc atccagcggg tcatcgagtt gaccttcgcc
     2821 gacgaggacg tgcgggtgaa ggccgtcagc acgggccagc aggccatcga cgaggtgtcc
     2881 tcggatccgc ccgacatcgt gctcgccgac gtgggcatgc ccgagcgcga cggctacgag
     2941 gtggccgcct tcgtcaaggg ccggcccgag ctggcccaca tcccggtgct gctgctgacc
     3001 ggcgccttcg agccggtgga cgacgctcgc gccgccgccg ccggctgcga cggcgtgctc
     3061 gccaagccgt tcgaacccca gatactcatc agccgcgtga aggagctgct ccccaaggcg
     3121 ggcgcgccgg cccgggccgg cgtgcacgcg gcggcagccg aggcggcccc cgcgccgtcc
     3181 ttcgacgccg tgccgcccgc gccccccggc gaagaggagg ccgccctcga catgcacgcc
     3241 ctgctcgact cgcagcccgg acgttcaccc gaggcggatg tctcgctcga cgactacttc
     3301 gaccgtctcg accaggcgtt cgcaagcctg gccgggggcg ccggcagggc ggggtctgtc
     3361 gcccccgaag ggtggggcgg gcccgccgag gcgccggcca ccgcgctcga acccgccggg
     3421 ccgccggccg tgccagccgc gcacgcgccc gcgccgccgc aggcgcttcg cgcggtccct
     3481 gccgccgatc tcttctccag cgttctcgag gccgaggccg cgggcgcgcc ggcggcccca
     3541 tcgccgctcg ccgtgttcgt gccgccgccg cccccggtga ccgacgagct ggtcgagcgg
     3601 atcgtgcgcc tggtgatcga acggatggcc gagcgcgacg tccgggaggc tctcgccgac
     3661 acggtgtcgc gggttgccga acggctcgtc cgggaggaaa tcgagcgcct gaaagcgctt
     3721 ccgcgctgat ccgggtcgcc gcccgccttg ccccggcccg gccgtcgggc ctcctcatcc
     3781 ttgcggcccg gtcgaaaccc cagtacgatc ttcagctgtg aagacaccgt tgccgaaggg
     3841 tttcgaccac gcggacgtcg atccgaagtg gtatgcgttc tgggagtcga tcggggcgtt
     3901 ccgcgccgac ccctcatccg acaagccgcg cttcagcatg gtgatcccgc cgccgaacgt
     3961 gacgggctcc ctgcacatgg ggcacgcgct caaccacacc ctgcccgaca tcgtcgcccg
     4021 gtggaagcgg atgcagggct acgacgtgct ctggctgccg ggaaccgacc acgccggcat
     4081 cgccacacag aacgtcgtcg agaagcagct ggccctcgag gggaagaccc ggcacgacct
     4141 cgggcgggag gcgttcgagg cgcgggtgtg ggagtgggtg cgccagagcc acggcacgat
     4201 caccgggcag atgcgcaagc tcggcgagtc ggtggactgg agccgcgagc ggttcacgct
     4261 ggacgagatg ctgtcgcgcg ccgtccggcg agtattcgtc tccctctatc aggacggcct
     4321 catctaccgc ggcacctaca tcgtgaactg gtgcccgcgg tgccgcaccg cgctctccga
     4381 cctggaggtc gtgcaccagc ccaaggcggc gcagctgtgg tacgtgcgct acccggggac
     4441 caagggcggc cgcggcgtga cggtggccac cacgcgcccc gagacaatgc tgggcgacac
     4501 ggccatcgcg gtgaaccccg gcgacgagcg gtaccgcgac ctggtgggcg ggcacgtgat
     4561 cctgcccatc atcgggcgcg agctgcccgt catcgccgac gagttcgtgg atccggcgtt
     4621 cggcacgggc gccgtgaagg tgacgccggc ccacgacccg aacgacttcc agatgggtca
     4681 gcggcacggc ctgccgcaag tggcggtcat cgacgaggac gcgcggatca ccgccgccgg
     4741 cggcccttac gcggggcagg accggttcgc ggcgcgcgaa gggatcgtgg cccagctcga
     4801 gcgcgagggg ctgctcgaga agatcgagcc ccacgagcac gcggtgggca cctgccagcg
     4861 ctgcggcacg gtggtcgagc ccctcgtgtc gacgcagtgg ttcgtgaagg tcgcgccgct
     4921 ggcgaaagag gcgatcaggg tggtggagca ggggaagatc cggttcttcc ccgagacctg
     4981 gaccaagacg tactacgact ggatgtacaa catccacgac tggtgcatct cgcgccagct
     5041 gtggtggggc caccgcatcc ccgcctggta ctgcgaggcg tgcggggaga tgatcgtctc
     5101 ggagcaggca cccgaaaggt gcccgtgcgg cgggacgctc aggcaggacc ccgacgtgct
     5161 cgacacctgg ttcagctccc agctctggcc gttcagcacg atggggtggc ccgaccgcac
     5221 cggggacctg gcgcggtact accccaccga cctgatgctg accgccttcg acatcatctt
     5281 cttctgggtc gcgcgaatga tcatggccgg cgtgcgcttc gccggcgaca tcccgttccg
     5341 cgaggtctac atcaccggcc tcgtccgcga cgagggcggc cggaagatga gcaagtcgaa
     5401 ggggaacgtc gtcgacccgc tggaggtgat ggacgagatc ggcgccgacg ccctccgctt
     5461 cacgctgacg gcgatggcgg cccccgggat ggacatcccg ctctccgagg ggcggatgca
     5521 gggctaccgg cagttcatca acaagatctg gaacgcgtcg agattcgtgt tgatgaacgc
     5581 cggcgagctg gaggagcggc ccgagatccc ggccggcgcg ccgctcgcgc tgatccaccg
     5641 gtggatcctg catcgcctga acgtggtgac cggcgaggtg caggagagcc tggagacgtt
     5701 ccggttcgac gtcgcggccg atcgcctcta tcacttcttc tgggacgagt acgcggactg
     5761 gtacatcgag atggtcaagc cccacctcca ggccgggggc gacgaggggc gcatcgcgca
     5821 ggcggtgctg ctcgaggtgc acgaccgcac gctccggctg ctgcacccga tcatcccgtt
     5881 cgtgaccgag gagctgtggc agcacctgcc gcgcagagcc ggcgacggcc ccacggtgac
     5941 gctcgccgcg tggccgaagg tccgccccga gtgggtggac gaggcggccg acgcggaggt
     6001 cggcctgctg cagggggtgg tgacgacgat ccggaccgcc cgggccgagg ggaaggtgaa
     6061 gccgtcggca aaggtcgggg tgacgctcga aggggcgggc gaggagcagg tgcggatcct
     6121 gcgggcgcag cagcgctacc tcaccacgct ggcaggcctc tcctcgttcg agttcggggc
     6181 cgaggcgccg ggcggcgaag acctggtgac gcgcgtgctc ggtgatctgc gggtccacat
     6241 ccagctgcct caccgcgacc gcggggccga ggtggagaag ctgaggaagc agctggccga
     6301 caagctccgc gaggtggctg gcgtggacgc gaagctcgcc aacgaagagt tcgtcgcgaa
     6361 ggcgccggcc cgggtggtgg aaggggcgcg ggcccagcgc gacaagctgc tcgtcgagca
     6421 gcggcgcatc gaggagacgc tgcgcgagct gggggagcca tgagccccgg ggagacgccc
     6481 gccgcggtgc ctcccgacct gatcgcggcc ctcgacgcgc gccgcgcgcg gctgggcggc
     6541 ttcgcagcct tcctgagata tttcaagagc gtcggctcga ccaacgacgt ggccgcgcgg
     6601 ctggcggacg cgggggcccc gcacggggcg gccgtcattg cggtggcgca ggaacggggc
     6661 cgcggccgca tgggccgcgg ctggttctcg cctcccggtt cgggtcttta cgtgtcggtg
     6721 gtcctgcgtc ccgcggctct cggcgcgggg ttcgggagcg gggcttccgg ggcgagcgtg
     6781 gcgtcctcgg tcaccctcag cgcgggcgtg gccgttgccg aagcgattcg cgaggtgtcg
     6841 ggcctgcggg tcgagatcaa gtggcccaac gatctcgtcg tgggacgccg gaaactgtgc
     6901 ggcatcctcg ccgaggcgag cgccgctgca tcgggtctcc agcacgtcgt gctcggcttc
     6961 ggcatcaacc tgcgggcggc cggctatcca cccgacctgg caggccgcgc cacctccatc
     7021 gaggaagaac tggggcggcc cgtcgacgag caggtgctgc tggccgaggc cctggcccgt
     7081 ctctcggaga ccatctcggg gctgccggtg cgaggcctcg aggccgtgct ccgccgctgg
     7141 cgtgaactgt ccccctccgc gtccggcgcg cgcgtggaag ttctcgcggg ggccggccgc
     7201 tgggaaattg cgaccaccgc gggaatcgac gacgacggcg ccctgctggt gacccgcgcc
     7261 ggctcgatcc accgcgtcat cgccggcgag gttcgctggc tctgaggccg ttccatgctg
     7321 ctggccctcg acatcggcaa caccaacatc gtcgtcggcg tgttcgacgg caacgacctt
     7381 ctccacagct ggcgtttgtc taccctgcgc cagcggaccg ccgacgagat gggcatctgg
     7441 ctggtgcagc tgttccggca cggcgggttg gaggtggggg gcgtcgacgg ggtggtgatg
     7501 gcgtcggtcg tgcccaccct gacgcggccg accctcgaga tggcgaagcg gtacttcggc
     7561 cacgcgccgc tggtggtgga cggcgaggcg aagaccggca tgcccgttct ctacgaggcc
     7621 cccgcggaag tcggggccga ccgcatcgtg aacggcgtcg ccgcgttcga gcggcacggg
     7681 acggcagacg gccgcccgcg cgcgctcgtc gtggtggact tcggcaccgc gacgaccttc
     7741 gacgccatca ccagccgcgg cgagtacctt ggcggcgtca tctgcccggg cgtgacgatt
     7801 tccgccgacg cgctgttcga gcgggccgcc cgcctgccgc gcgtggacgt gcgcaagccg
     7861 gccgcggtca tcgggcggac gaccgtcggg tcgatgcagt ccggcctgtt ctacgggtac
     7921 gtcgggctgg tcgaggggat cgtcgagcgg atgaagcgcg agctgggggc gccggcgggc
     7981 tgcgtggcga cgggcggcct cgcagagatg atcgcccccg agactccggc catcgaggcc
     8041 gtggaccgcg acctgacgct gcacggcctg cggatcatct gggacaggaa cagggggtag
     8101 gggcgagtgg ccgggatgga tcccgatcag tactgccgcg agatcgagtc ttacctgtgc
     8161 cggcgcaacg ccgggcacct ggtgcgcctg gtcggccccg ccttcgagat ggtggcgggg
     8221 tgggcgagga ccggcatccc gatccgcgta gcctgcgccg gcatcgaccg ctacctcgat
     8281 cgccacgacg cgaaggcggg cggcgcgccg gcggcgcgcc ggcggcccgt gcgggcggag
     8341 ttctgcgagg ccgacgtcct ggacgcgttc gaccagtggc ggcgggccgt gggcctcggc
     8401 acggctggca gcgccgcgcc cgaagccgcg gcgggcggcg ggggacacca gtcgctcaga
     8461 gcgcacctcg atcgggtggc cacccgcctt accgatcgat tggccgacgg atcggtcgcg
     8521 ccgatcctgg aggcgcacct cgagcgcctg ctcgacgagc tgtcgcggat gcgcgaaggg
     8581 gccaggaccg tgcgggggga ggcgaggcaa caagtgatcg cccggctcga ggaactcgat
     8641 cgggcgctgc tggaggcggt gcaggccgcg gcgggggccg agctgctgga gtcggccacg
     8701 gcggaggcgg aggcggatct gggcgacttc cgcacgcgga tggcggcggc cgagtacgcg
     8761 cgggcgcgcg acgccgccgt ccgggcgttg ctgcgcgatc gctgccggct tcccgtcatc
     8821 gcttattgag aatcggatga gatttggggc caggccccat ttcccgatcc gcggggccgc
     8881 cgcccttcgc gggtcccggt tcgcttgttg agaccgaggt gagaaatggg gcctggcccc
     8941 ggggctgcgc tactcagggc agggaacgtc gtcgcgctgg atgtcgagaa accggcggcg
     9001 ggggggcgga tgctcgcccg tcacgacggg ctcgtcgtcc tcgtcagcgc cgccatccct
     9061 ggcgagcagg tgcgtgcgct cgtccagcgc accagcgccg gcgtcgccta cgcggtcacc
     9121 gtcgaggtgg tcaaggccag ccccgatcgg cggccggccg ccaccgactg ggcctgcggc
     9181 ggcaacgtct acgcgcacat cgcctacccc cgccagctct cgctcaaggc cgagatcgtg
     9241 ctcgacgccc tggcccggat cggccacatc acccaccagg cgccgatcga ggtgagcccc
     9301 tcgcccgagc gaggctaccg gatgcgggcg cggttccacc tccgcgggtc gcacatcggg
     9361 ttctttcgcg agggcaccca ccacttgtgc gacccggcct ccaccggcca gctcctgccc
     9421 gagacggacc gggtactggc cggcatcggc gagatcctgc ggacgggccg cctccagggg
     9481 gtgacgagca tcgagctggc ggagaatgtt cccgcaacgg agcgggcgct gcacctcgag
     9541 ttggagcgac ccgtcccgcc ggccgggctc gagctgctgg cgcgtatccc gaccgtggtg
     9601 ggggtgagcg cgtcgttcgg cggagcgtcc ccttcggcgg gccgccatcc gggccagggc
     9661 caggggtggc gccgcaccat gaccgccggc ggggcggcct gcgtgctgga cgatcttgcc
     9721 ctgcccgaca cgcctccgtt ccgcctgcag cacaacgccg ccgccttctt ccaaggcaac
     9781 cgctacctcc tggcgccgct ggcggcgagg gtttgctccc tggttccgcg gggaccggtc
     9841 atcgacctgt atgcgggcgt cgggctcttt gccgcggccc tcgccgcctc gggctgggag
     9901 cgcgtggtcg ccgtggagag cgaccgggcc gccgccgccg acctgcgggc caacgcggcc
     9961 ccgttcggcg cggccctcga gccggtggag atgccggtgg aagactacct ggccgggcac
    10021 cagtcggacg gcgca
//
LOCUS       DAOPQX010000108        10033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7637_length_10033_cov_4.876228, whole genome shotgun sequence.
ACCESSION   DAOPQX010000108 DAOPQX010000000
VERSION     DAOPQX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7637_length_10033_cov_4.876228"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKK95_07640"
     CDS             complement(<1..797)
                     /locus_tag="PKK95_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOC18124.1"
                     /translation="MSDGPPDTMNVFETRIQADSERFRSNRERMARLVAELRERLAEA
                     RRGGGARALERHRDQGKLPVRERVERLLDPASPWLELSPLAAFEMYGHEAPAAGLVTG
                     IGRVSGREVVVIANDATVKGGTYFPITVKKHLRAQQVALENRLPCMYLVDSGGAFLPL
                     QAEVFPDREHFGRIFFNEARMSAERIPQVAVVMGSCTAGGAYVPAMADETVIVRGTGT
                     IFLAGPPLVKAATGEDVTAEELGGADVHTRLSGVADYLAEDDDHALA"
     gene            997..2400
                     /locus_tag="PKK95_07645"
     CDS             997..2400
                     /locus_tag="PKK95_07645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC18125.1"
                     /translation="MTVKRKKRVLVIDDEPAMTDWLKAVLEHGGYEVRTALIGARGEE
                     IFRSWRPDVAVTDMVLPDVDGIDLLHRFKESQPETEVVVISGHGTISKAVSAIKAGAS
                     YFLEKPIDSDSLLTLLEKAMERKDLQTENLQLKQKLQDKFKFANIIGKSKKMQELFEL
                     IESVAASEANILIQGENGTGKELIANAIHYNSKRSKGPFIKINCAAIPKDLIESELFG
                     YKKGSFTGATMDKEGLFEMAEGGSLLLDEIGEMPSYLQTKLLRVLQEREYRPIGSDRI
                     VHVDFRLITATNIDLDAALREGKLREDLYFRINTITLRVPPLRERTEDIPLLCEHFLE
                     KFSQRYQKNIRGFSPAAYHVLIRNRWPGNVRELENAIERAVLVSKGSELQPSDLPETL
                     REETAAPADFAFPPHWTLAEIEKMAILQTLQRTNWNKQEAAAILGLYRPTLYSKMKKH
                     EIRDMGRAARQQAAAER"
     gene            2464..3249
                     /locus_tag="PKK95_07650"
     CDS             2464..3249
                     /locus_tag="PKK95_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18126.1"
                     /translation="MIDRLGRSVLDALAAVGEVSMFAGRALRDTFLPPYEFGEFFRQL
                     YELGWRSAPLIAASGVAIGVVMSMHTRSTLERFGAEAMIPAGLGLSVVAEIGPLITAL
                     LMAGRAGAGIGAELGAMKVTEQIDALEAVAVDSFRYLSVTRVAAAVVALPLLTVLMDF
                     AAIVGGYLAEMLVSGMSFGLYLTRTFTYIDFSDVLPSTLKTAVFGFIIAVTGSYLGFT
                     TSRGTEGVGHASTRAVVASSILIIVADVVLVRIIMFFFPRGMV"
     gene            3246..3992
                     /locus_tag="PKK95_07655"
     CDS             3246..3992
                     /locus_tag="PKK95_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC18127.1"
                     /translation="MSGGVMVRLDGVHKSFGGRRVLDGVSFEVPRGRAFCLLGRSGSG
                     KSVTLRHIVGLVRPDEGDVQVAGRDVTRLAGRELAEARRGIGFLFQGGALFDSLSVGE
                     NVAFPLRRHTRLTGAEIRARAQERLEEVGLAREYEKMPAALSGGMRKRVALARAMALD
                     PAVLLVDEPSAGLDPITSEEIDRLLLERKERAGVTLVVVTHNIPSARLLGDQLAVLHE
                     GRIVARGTAAELARSDHELVRAFMQSGAGG"
     gene            3994..5328
                     /locus_tag="PKK95_07660"
     CDS             3994..5328
                     /locus_tag="PKK95_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOC18128.1"
                     /translation="MQTTRAVGVGVFVLIGLALFAAILFLIGDRRGLFSDSFTAYTDL
                     QALSGVAVGTPVWVSGMSAGEVKEIVVPPRPGAPFRLRLEVDETLHQIVRTDSVVSVR
                     TEGLVGGRFLQISAGSEAAPRLPDGGVLRGQEPFDVAEMLDQIRLVVDNFNTTIVTLR
                     GQFEQAIASVEQAAAEATGLVRGASDDVQRIAEAGRRIVENARAISDRVRAGQGTVGR
                     LIEDDGLYRDVAAIAQEARLTAENIRAFSEHARGAMKSVEAKAQGTQPLGASLQATLD
                     RTQEAMTNLADATEALKHNFLLRGYFRNRGYFNLSDLSESDYRAGALERRGRRALRIW
                     LSAGVVFETGPDGQETIGEGGRRRLDSAMGGFLEYREAGPLIVEGYATSSDEAERYLV
                     SRTRAALVREYLVRRFDLDPSAVGFLALGNRPVGEPPSTPWDGIALAVFVPR"
     gene            5495..5779
                     /locus_tag="PKK95_07665"
     CDS             5495..5779
                     /locus_tag="PKK95_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18129.1"
                     /translation="MNVDERTRVFLMAMAGAVAGAVAGYLYFTERGKQFLASWEPKLD
                     NAMREMGHLRETVTKAQAVASEGWRSISQITSSGREGKGGDWGGRQSSPF"
     gene            5808..6179
                     /locus_tag="PKK95_07670"
     CDS             5808..6179
                     /locus_tag="PKK95_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOC18130.1"
                     /translation="MANEYDYESGSGGSGFVVGLLTGTVLGAGLGMLFAPKSGSELRG
                     KISESATHMGEKGRVIVDKARDAVSRGSEEARRLGEKASHMASSATGSSASGSSSNIG
                     GSSSPGGSNPGSPYSSGPTRG"
     gene            6243..7181
                     /locus_tag="PKK95_07675"
     CDS             6243..7181
                     /locus_tag="PKK95_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOC18131.1"
                     /translation="MRQAVAEARRVSQLAARAGSALKSWVILIGKAFWRGSLEFYQSD
                     DLTHAASIGYYALLSIFPFFLLAFSALGGLTASEENRVTILSFLLRYFPTQFEFITRQ
                     LDSLQQMRVRIGIGGLIALAWAALGFFGAISTAVNHAWGVEKQRSYLKHKLFSFLMLL
                     AAGVLLAVAVLLAGAVQIIDATWYPALVARMPGVAVLQSLLLRWAATLMLILVVGLIY
                     YFVPNAKVRFRDVWPGAILTGLLWRAALEGFSFYVRDMSRFTFVHGSIAAVVVFLIWV
                     YTCAVVLLYGVEFTAAYARLRRGRPEQVPAAPTPRT"
     gene            7226..9385
                     /locus_tag="PKK95_07680"
     CDS             7226..9385
                     /locus_tag="PKK95_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOC18132.1"
                     /translation="MNRLTSSNSPYLLQHAANPVDWYPWGEEAFAAARRSDRPIFLSV
                     GYSTCHWCHVMAHESFESEQIAALLNEHFVPVKVDREERPDVDRVYMTFVQATTGQGG
                     WPMSVFLTPDLQPFYGGTYFPPDSRYGRPGFRELLRELARLWREDRERVLASARSVHE
                     QLARATASAPGPRVPPVTVLARAVEELGSQFDDRYGGFGRAPKFPRPSELLFLLREHA
                     RSGDARALAMVTATLCAMSDGGMRDQVGGGFHRYSVDGQWRVPHFEKMLYDQAQIVLA
                     LLEAAQASGDLSLLEVAEDTLAYVARDLADPAGGFCSAEDADSLPPGAAEGAHPTEGA
                     FYLWGADEIDRLLGADAEIAKTCFGVEPEGNALHDPQGEFRGRNILYRAATDEEAARR
                     HGVERAEVASARERARRVLFEARASRPRPGLDDKVITAWNGLAIAAFARASRVVAALH
                     PDAAPRAAAYLESARRAAAFLRERAWDEPRGRLMRRYRRGEAGVDGYAEDYACLVFGL
                     IELFQAGGDPEDLRFALRLQRRQDELFWDEAGGWFGTTGEDSSMLLRLKEEYDGAEPA
                     ASSVGLLNLIALSQLGPADGGAAVDPPGAGPAATGGLPARIERTLQAFEPRLSHASRA
                     LPMMLAALSAYHADRQEIVVVGEAHDPRTRALIRAVESRYRPFAIVIRLEPGKAADLA
                     RDLPHVAGMGMRGGLPAAYICRNFTCDTPITDPAALA"
     gene            9462..9662
                     /locus_tag="PKK95_07685"
     CDS             9462..9662
                     /locus_tag="PKK95_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18133.1"
                     /translation="MLTFLLWLLLLFCCWPLALLALVLYPFVWLLLLPFRILGIAVDG
                     VLGLLRGVVLLPARALGAVRSR"
     gene            9706..>10033
                     /locus_tag="PKK95_07690"
     CDS             9706..>10033
                     /locus_tag="PKK95_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC18134.1"
                     /translation="MSGPLPIAIDKLGKRYGRAQALAGLSLEVREGEVFGFLGLNGAG
                     KTTTIRLLLDLIRPTEGGARIFGHDCRAASIEVRRLVGYLPGDPPFLADMTGEHLLRL
                     LERVGGS"
BASE COUNT         1447 a         3395 c         3614 g         1577 t
ORIGIN      
        1 aacgccagcg cgtggtcgtc gtcctccgcc aggtagtcgg ccacacccga cagccgcgtg
       61 tgcacgtcgg cgccgcccag ctcttcggcc gtgacgtcct cgcccgtcgc cgccttcacc
      121 agcggcggcc cggccaggaa gatcgtcccg gtgccccgca cgatgaccgt ctcgtcggcc
      181 atggccggca cgtacgcgcc gccagccgtg cacgagccca tcaccaccgc aacctgcggg
      241 atgcgctcgg ccgacatccg tgcctcgttg aagaagatcc ggccgaagtg ctcccggtcg
      301 ggaaagacct cggcctgcag cggcaggaac gccccgcccg aatccaccag gtacatgcag
      361 gggagccggt tctcgagggc tacctgctga gcccgcaggt gcttcttcac cgtgatcggg
      421 aagtaggtgc ctcccttcac ggtcgcatcg ttggcgatga cgacgacctc acggccgctc
      481 acgcgtccga tcccggtcac cagcccggcg gccggcgcct cgtggccgta catctcgaag
      541 gctgccagcg gcgacagctc gagccacggg gacgccgggt ccagcagccg ctcgacccgc
      601 tcgcgcaccg gcagcttccc ctgatcgcgg tgacgctcga gggcccgcgc ccccccgcct
      661 cgccgcgcct cggccagccg ctcccgcagc tcggccacca gccgcgccat ccgctcccgg
      721 ttggagcgga atcgctcgga atcggcctgg atccgggtct cgaagacgtt catcgtgtcg
      781 ggtggcccgt cagacattat tccagattgt ggctgttttt cctcgtacga aaaccgaaca
      841 gagtgtcgaa taggtgacat tcggggttca gtgtgtataa tttccgcggg cttcattcac
      901 cgcctccccc cattcattct gcagaggcag cacggcaacg gccagcgacg agcgggcccc
      961 gccggatggg ggagggcagt tggttttcga gggcgcatga ccgtcaaacg caagaagcgc
     1021 gtgctcgtca tcgacgacga gccggcgatg accgactggc tgaaagccgt gctcgagcac
     1081 ggcggctacg aggtgcggac ggccctgatc ggggcccgcg gcgaggagat cttccgcagc
     1141 tggcgccccg acgtcgccgt caccgacatg gtgcttcccg acgtggacgg gatcgacctg
     1201 ctgcaccgct tcaaggagag ccagcccgag accgaggtgg tggtcatctc gggccacggc
     1261 accatctcga aggcggtgtc ggccatcaag gcgggggcgt cgtactttct cgagaagccg
     1321 atcgactcgg acagcctgct gacgctgctc gagaaggcga tggagcggaa ggacctgcag
     1381 accgagaacc tgcagctcaa gcagaagctg caggacaagt tcaagtttgc caacatcatc
     1441 ggcaagagca agaagatgca ggagctcttc gagctcatcg agagcgtggc ggcctccgag
     1501 gccaacatcc tgatccaggg cgagaacggc acggggaagg agctgatcgc caacgcgatc
     1561 cactacaaca gcaagcggtc gaaggggccg ttcatcaaga tcaactgcgc cgcgatcccc
     1621 aaggacctga tcgagtcgga gctgttcggg tacaagaagg ggtcgttcac cggcgcgacg
     1681 atggacaagg aggggctctt cgagatggcg gaggggggct cgctgctcct cgacgagatc
     1741 ggcgagatgc cctcctacct gcagaccaag ctgctgcggg tgctccagga gcgcgagtac
     1801 cggccgatcg gcagcgaccg gatcgtccac gtcgacttcc ggctcatcac ggccaccaac
     1861 atcgatctcg acgccgcgct ccgcgagggc aagctgcggg aggacctcta cttccggatc
     1921 aacacgatca cgctgcgggt gcccccgctg cgcgagcgca ccgaggacat cccgctgctc
     1981 tgcgagcatt tcctcgagaa gttctcgcag cgctaccaga agaacatccg cgggttctcg
     2041 ccggcggcct accacgtgct gatccggaac cgctggccgg gcaacgtccg cgagctcgag
     2101 aacgcgatcg agcgggcggt gctggtgtcg aaggggtccg agctgcagcc gtccgatctg
     2161 cccgagacgc tgcgcgagga gacggccgcg ccggccgact tcgccttccc gccgcactgg
     2221 acgctggccg agatcgagaa gatggcaatc ctgcagacgc tccagcggac gaactggaac
     2281 aagcaggagg cggcggccat cctcggcctc taccggccca cgctgtacag caagatgaag
     2341 aagcacgaga tccgggacat gggccgggcg gcgcgtcagc aggccgcagc cgaacgctga
     2401 gccggcccgc ggttggtcag tcttaccgct gaggttggcc cgtttttcgc atcgatctct
     2461 gccgtgatcg accggctcgg ccgctcggtg ctcgatgcgc ttgccgcggt gggcgaggtg
     2521 tcgatgttcg ccggccgcgc ccttcgcgac accttccttc ccccctacga gttcggcgag
     2581 ttcttccgtc agctgtacga gctgggctgg cgctctgcgc cgctcatcgc cgcttcgggc
     2641 gtggcaatcg gcgtggtgat gtcgatgcac acccgctcca cgctcgagcg gttcggcgcc
     2701 gaggcgatga tcccggccgg cctcgggctg tcggtcgtgg ccgagatcgg cccgctgatc
     2761 acggcgctgc tgatggcggg gcgcgcgggc gccgggatcg gagccgagct gggggcgatg
     2821 aaggtcaccg agcagatcga cgcgctcgag gccgtggcgg tcgactcgtt ccgctacctg
     2881 tcggtcacgc gcgtggccgc ggcggtggtc gcgctgccgc tgctcacggt gctcatggac
     2941 ttcgccgcga tcgtgggggg atacctggcc gagatgctgg tgagcggcat gtcgttcggg
     3001 ctgtacctga cgcggacctt cacctacatc gacttctcgg acgtcctgcc ctcgacgttg
     3061 aagaccgcgg tgttcggatt catcatcgcc gtgaccggct cgtacctcgg ctttaccacc
     3121 tcgcgcggca ccgagggggt gggccacgcg tccacccgcg ccgtcgtggc ctcgtccatc
     3181 ctgatcatcg tcgccgacgt cgtcctggtg cgcatcatca tgttcttctt cccgcggggg
     3241 atggtgtgag cggcggggtg atggtgcgcc tcgacggggt gcacaagtcg ttcggcggac
     3301 gccgggtgct cgatggcgtg tcgttcgagg tgccgcgcgg gcgggcgttc tgcctgctgg
     3361 gacgcagcgg ctcgggcaag agcgtgacgc ttcggcacat cgtcggcctc gtccggcccg
     3421 acgagggaga cgtgcaggtg gcggggcgcg acgtgacgcg cctggcgggg cgcgagctgg
     3481 cggaggcgcg gcggggaatt ggctttctct tccagggcgg ggcgttgttc gactcgctca
     3541 gcgtgggcga gaacgtggcg ttccccttgc ggcggcacac gcggctcacg ggcgccgaga
     3601 tccgggcgcg cgcccaggag cggctcgagg aggtggggct ggcccgcgag tacgagaaga
     3661 tgccggcagc cctctcgggc gggatgcgga aacgggtggc gctggcgcgg gcgatggcgc
     3721 tcgatcccgc ggtgctgctg gtggacgagc cgagcgccgg cctcgacccg attacctcgg
     3781 aggagatcga tcggctgctg ctcgagcgga aagagcgcgc cggcgtcacg ctggtcgtcg
     3841 tgacgcacaa cattccaagc gcccgcctgc tcggcgacca gctggcggtg ctccacgaag
     3901 ggcgcatcgt cgcgcgcggc acggcggccg agctcgcgcg cagcgatcac gagctggtgc
     3961 gggcgttcat gcagtcgggc gcgggagggt agcgtgcaga ccacgcgcgc ggtgggcgtc
     4021 ggcgtgttcg tcctgatcgg cctggcgctg ttcgcggcca tcctgttcct gatcggcgac
     4081 cgccggggcc tcttctccga cagcttcacg gcctacaccg acctgcaggc gctgagcggg
     4141 gtggcggttg gcaccccggt gtgggtctcg gggatgagcg cgggcgaggt caaggagatc
     4201 gtcgtgccgc cgcgccccgg cgccccgttc cgcctccgcc tcgaggtgga cgagacgctg
     4261 caccagatcg tgcggaccga ttcggtggtg tcggtccgga ccgaggggtt ggtgggcggg
     4321 cgcttcctgc agatcagcgc gggatccgag gcggcccccc gcctgccgga cggcggcgtc
     4381 cttcgagggc aggagccgtt cgacgtcgcc gagatgctcg accagatccg cctcgtggtg
     4441 gacaacttca acacgaccat cgtgacgctc cgcggccagt tcgagcaggc gatcgccagc
     4501 gtcgagcagg cagccgccga ggcgaccggc ctggtgcgcg gggcctcgga cgacgtgcag
     4561 cggatcgccg aggcgggccg ccggatcgtg gagaacgcgc gcgcgatctc cgatcgggtg
     4621 cgggcggggc aggggaccgt ggggcgcctg atcgaggacg acgggctcta ccgcgacgtg
     4681 gcggccatcg cgcaggaggc gaggctgacg gccgagaaca tccgcgcctt ctccgaacac
     4741 gcccgcgggg cgatgaagtc ggtggaggcg aaagcgcagg ggacgcagcc gctcggcgcg
     4801 agcctgcagg cgacgctcga tcggacgcag gaggcgatga ccaacctggc cgacgccacc
     4861 gaggcgctca agcacaactt cctcttgcgg ggctacttca ggaaccgcgg ctacttcaac
     4921 ctgtccgacc tgtccgagtc ggactaccgc gcgggggcct tggagcggcg cggccggcgg
     4981 gcgctgcgca tctggctgtc ggccggcgtg gtgttcgaga cggggcccga cgggcaggag
     5041 acgatcggcg agggggggcg gcgccgactg gattccgcga tgggcgggtt cctcgaatac
     5101 cgcgaggcgg gccccctcat cgtcgagggg tacgcgacct cctcggacga ggcggagcgg
     5161 tacctcgtgt cgaggacgcg agcggccctg gtgcgtgagt acctcgtccg caggttcgac
     5221 ctcgatccgt ccgcggtcgg gttcctcgcc ctggggaacc ggcccgtggg tgagccgccc
     5281 tcaacgccct gggacggcat cgcgctggcc gtgttcgtgc cccggtaact ccattacacc
     5341 tttgccttcc cacgtgtgct gatccgcgac gcggccgcgg agggcaccgc acgcgcattt
     5401 tctcccattc tggcccgaat cctccgcgag ccggtccgcg gccgcgtggc acgggagttg
     5461 attcgctttc accggactca agaacggagg cgacgtgaac gtggacgagc gcacgcgtgt
     5521 gttcctgatg gcgatggcgg gggcggtcgc gggcgccgtg gccggctatc tctacttcac
     5581 cgagcgaggg aagcagttcc tggcgagctg ggagcccaag ctcgacaacg cgatgaggga
     5641 gatgggccac ttgcgcgaga cggtcaccaa ggcgcaggcg gtggcgagcg agggctggcg
     5701 gtcgatcagc cagatcacca gctccgggcg tgaaggcaag ggaggcgact ggggtggccg
     5761 gcagtcgtcg ccgttctagc ggaacagctt tcctgaagga gaatcacatg gcgaacgagt
     5821 acgattacga gtcgggcagc ggcgggtcgg gtttcgtggt gggactgctg acgggcaccg
     5881 tgttgggtgc gggcctcggc atgctgttcg ctccgaagag cggctcggaa ctgcgcggca
     5941 agatcagcga gtcggccacc cacatgggcg agaagggccg cgtgatcgtg gacaaggcgc
     6001 gcgacgccgt gtcgcggggc tcggaagagg cccggcgcct gggtgagaag gcgtcgcaca
     6061 tggcaagctc ggccaccggc tcgagcgcga gcgggtcgtc gtcgaacatc ggcggcagca
     6121 gctcgccggg cggctcgaac ccgggctccc cctattcgtc gggtccgacc cgcgggtaag
     6181 cgccctcggc cgggcccggg ggaccacccc cgggtcccgg tccgctggtg cacaataggc
     6241 gggtgcgaca ggctgtggcc gaggcccggc gcgtttccca actcgcggca agagccggat
     6301 cggcgctgaa gtcgtgggtg atcctgatcg gcaaggcgtt ctggcgggga tcgctggagt
     6361 tctaccagag cgacgacctg acgcacgcgg cctcgatcgg ctattacgcg ctgctctcga
     6421 tctttccttt cttcctgctg gccttctcgg ccctcggcgg cctcaccgcg agcgaggaga
     6481 accgcgtcac catcctcagc ttcctgctcc ggtacttccc gacacagttc gagttcatca
     6541 cgcggcagct cgactcgctt cagcagatgc gggtgcgcat cggcatcggc gggctgatcg
     6601 cgcttgcgtg ggccgcgctg ggcttcttcg gggccatcag cacggcggtg aaccacgcat
     6661 ggggcgtcga gaagcagcga agctacctga agcacaagct gttctcgttc ttgatgctcc
     6721 tggcggcggg cgtgctgctc gcggtcgcgg tgctgctggc aggggcggtg cagatcatcg
     6781 acgccacctg gtaccccgcc ctggtggcgc ggatgccggg ggtcgcggtg ctgcagagcc
     6841 tgctgctgcg atgggccgcc acgctgatgc tgatcctcgt cgtggggctg atctactact
     6901 tcgtgccgaa cgcgaaggtc cgcttccggg acgtctggcc gggcgcgatc ctcacggggt
     6961 tgttgtggcg cgcggcgctc gaaggattct ccttctacgt gcgcgacatg agccgcttca
     7021 ccttcgtgca cgggtcgatc gccgcggtgg tggtgttcct catctgggtg tacacgtgcg
     7081 cggtggtgct gctgtacggc gtggagttca cggcggcgta cgcgcggctg cgccgcggcc
     7141 gccccgagca ggtgcccgcg gcccccacgc cgaggacctg aaaaccaaga actaagaact
     7201 aagaactaac aactaagagc cccccgtgaa ccgcctcacc tcctccaaca gcccgtacct
     7261 cctgcagcac gccgccaacc cggtcgactg gtatccctgg ggcgaggagg cgttcgccgc
     7321 ggcgcgtcgc agcgatcggc cgatcttcct gtcggtcggc tactcgacct gccactggtg
     7381 tcacgtgatg gcgcacgagt cgttcgagtc ggagcagatt gcggcgctgc tcaacgagca
     7441 cttcgtgccg gtgaaggtcg atcgcgagga gcggcccgac gtggaccgcg tgtacatgac
     7501 cttcgtgcag gccaccacgg ggcagggcgg ctggccgatg agcgtcttcc tgacccccga
     7561 cctccagccc ttctacggcg gtacgtactt tccgcccgat tcacgctacg ggcggccggg
     7621 attccgcgaa ctgctgcgcg agctggcgcg gctgtggcgc gaggaccggg agcgcgtgct
     7681 ggcgtcggcg cgcagcgtgc acgagcagct ggcgcgggcc accgcctcgg cgcccggacc
     7741 ccgcgtgccg ccggtgaccg tgctcgcgcg ggccgtcgag gagctcggca gccagttcga
     7801 cgaccgctac ggcggcttcg gccgcgcccc gaagttcccg cgtccctccg agctgctctt
     7861 cctgctgcgc gagcacgcgc gctcgggcga cgcgcgggcg ctcgcgatgg tgaccgccac
     7921 gctctgcgcg atgagcgacg gcggcatgcg cgaccaggtg gggggcggct tccaccgcta
     7981 ctcggtggac gggcagtggc gggtgcccca tttcgagaag atgctgtacg accaggcgca
     8041 gatcgtcctc gcgctgctgg aggcggcgca ggcgtccggc gacctttcgc ttctcgaggt
     8101 ggccgaggac acgctggcct acgtcgcacg cgacctggcc gaccccgccg gcgggttctg
     8161 ctcggcggag gatgccgaca gcctgccgcc cggtgccgca gagggggcgc acccgaccga
     8221 gggcgccttc tacctgtggg gggcggacga gatcgaccgc ctgctcggcg ccgacgccga
     8281 aatcgcgaag acgtgcttcg gcgtcgagcc agaggggaac gcgctgcacg atccgcaggg
     8341 cgagttccgc gggcgcaaca tcctctaccg cgccgcgacc gacgaggaag cggcgcggcg
     8401 tcacggcgtc gagcgcgccg aggtggcgtc ggcgcgcgag cgggcgcggc gcgtcctgtt
     8461 cgaggcaagg gcatcccgcc cgaggcccgg gctcgacgac aaggtcatca ccgcctggaa
     8521 cgggctggcg attgccgcct tcgcgcgggc gtcgcgagtc gtggccgcgc tgcaccccga
     8581 cgccgccccg cgggccgccg cctacctcga atccgcgcgg cgcgccgccg cgttcctccg
     8641 cgaacgcgcg tgggacgagc cgcgcggccg gctgatgcgg cgctaccgga ggggcgaggc
     8701 cggcgtggac ggctacgccg aagactacgc ctgcctggtc ttcggcctga tcgagctgtt
     8761 ccaggcgggc ggcgatccgg aagacctgcg cttcgcgctc cgcctgcagc gccggcagga
     8821 cgagctgttc tgggacgagg cgggggggtg gttcggcacc acgggcgagg attcgtcgat
     8881 gctgctgcgc ctgaaggagg agtacgatgg cgccgagccg gcggccagct cggtcggcct
     8941 gctcaacctg atcgccctct cgcagctcgg accggcggac ggcggagccg ccgtcgaccc
     9001 gcccggcgcc gggccggccg ccaccggcgg cctgccggcc cggatcgagc gaacgctgca
     9061 ggcgttcgag ccgcggctct cgcacgcgtc gcgcgcgctg ccgatgatgc tcgcggcgct
     9121 ctcggcctac cacgccgacc gccaggagat cgtggtggtg ggcgaggcgc acgacccgcg
     9181 cacccgcgcg ttgatccgag ccgtcgagtc gcgctaccgg ccattcgcga tcgtcatccg
     9241 cctcgagcct ggcaaggctg ccgacctcgc gcgcgacctt ccgcacgtgg cgggcatggg
     9301 gatgcgcggc ggcctgccgg ccgcttacat ctgccgcaac ttcacctgcg acaccccgat
     9361 caccgatccc gcggcactgg cctgaagacg cgcgccgatc cagaatgtga actccccgct
     9421 gccgggcttc gtctgattca gtgacgggca ggaggaaccg gatgctcaca ttccttctct
     9481 ggttgctgct tctcttctgc tgctggcccc tcgcgctgct cgccctggtg ctgtacccct
     9541 tcgtctggct cctcctgctg ccgttccgga tcctggggat tgccgtcgac ggggtgctcg
     9601 gcctgctgcg cggcgtggtc ttgctgccgg cgcgcgcgct cggcgccgtc cgcagcaggt
     9661 agagcagggc tttggtcccg ttccagacca gagtagactc tggccgtgtc cggacccctc
     9721 ccgatcgcca tcgacaagct tggcaagcga tatggccgcg cgcaggcgct ggccggcctc
     9781 tcgctcgagg tgcgtgaggg cgaggtgttc ggcttcctcg gcttgaacgg cgccggcaag
     9841 accaccacga tccgcctgct gctcgacctg attcggccta ctgaaggagg cgcgcggatc
     9901 ttcgggcacg actgccgcgc ggcctcgatc gaggtgcgcc gcctggtggg ctacctgccg
     9961 ggcgatccgc cgttcctcgc cgacatgacc ggcgagcatc tgctccggct gctcgagcgg
    10021 gtgggcggca gcc
//
LOCUS       DAOPQX010000109         9958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7713_length_9958_cov_4.349490, whole genome shotgun sequence.
ACCESSION   DAOPQX010000109 DAOPQX010000000
VERSION     DAOPQX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7713_length_9958_cov_4.349490"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2117)
                     /gene="treY"
                     /locus_tag="PKK95_07695"
     CDS             complement(<1..2117)
                     /gene="treY"
                     /locus_tag="PKK95_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HOC18135.1"
                     /translation="MPNPPDTPLATYRLQFHGGFTFRDAAALAPYLAALGVTHCYSSP
                     YLTAKPGSTHGYDICNHRELNPEIGSRADYDAFVRALHDHGLGQIIDFVPNHMGIDPR
                     TNPWWRDVLENGQSSPFARYFDIDWDPIKPELRGKVLLPILGDQYGLVLERGELRLGF
                     EDGALFLEYFDNLLPVNPRQAVMVYEHGLEEMTASLGEEHAALREFLSILTALRNLPA
                     IQETDPARIAERRREKEVARERLGRLISSSAEIRSHVERAVRFFNGTPGAPASFDPLH
                     ELLERQAYRLSDWRSASHEINYRRFFDINALAALRMEDPEVFEATHALVGELLDGGQV
                     DGLRIDHPDGLFDPKAYFEKLQELRGGRRRLWVAAEKILSGEETLPGDWPIAGTTGYA
                     FANDLTGLQIDPAGERPLRQFYARFTGFTWPFAEIAYRSKKLITQASLASELNVLAHA
                     LNRISESNRRSRDFTLESLRRVLQEVVACFPVYRTYIDQGGGTPADRTRVEEAVAVAK
                     RRNPTIPASQFDFLREAVLPRRLSPEAAFEDRRDGYAPVDEEGYQARLRFSMKLQQFT
                     APVQAKGVEDTAFYRYNLLVALNEVGGEPSRFGRRPEEVHARNQARARHWPEEMLTTA
                     THDTKLGEDVRARIAVISELAPDWRRHVSRWARINAGHRTEINGRPAPDRNDEYRFYQ
                     VLLGVWPSPPPSSTGVSPVAPSST"
     gene            complement(2141..5458)
                     /gene="treS"
                     /locus_tag="PKK95_07700"
     CDS             complement(2141..5458)
                     /gene="treS"
                     /locus_tag="PKK95_07700"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HOC18136.1"
                     /translation="MAEPLWYKDAVIYEVHVKAFLDSNGDGIGDFAGLASKLDYLEAL
                     GITCIWLLPFYPSPLRDDGYDISWYQGVHPAYGTLRDFRRFLDEAHRRGLYVLTELVM
                     NHSSDQHPWFKRARRMPRGSREREFYVWSDTKQRYEDARIIFTDTETSNWTWDFDAGA
                     YYWHRFFHHQPDLNYDNPEVRRRMRGRMDFWLEMGVDALRLDAVPYLIEREGTNCENL
                     PETHQILKEFRAHVDAKYPHRMLLAEANQWPADVRPYFGDGDECHMAFHFPLMPRMFM
                     ALQQEDRHPITEILHQTPDIPEVCQWALFLRNHDELTLEMVTDEERDYMYRAYAADTR
                     MRLNLGIRRRLAPLVENNRRRIELLHGLLFSLPGTPVLYYGDELGMGDNIYLGDRNGV
                     RTPMQWTSDRNAGFSRADPAALYAPPIMDPVYGYQAINVEAQERSPHSLLQWFRRMLA
                     LRRQHRTFGRGATRFLHPANRKVLAYVREGDGKRIFVIANLARSVQPVELDLSEFKGL
                     TPVEMLGRTEFPRIGELPYFLTLGPYAFYWFELVEQPAPISARVVPQPAESPAEFPAL
                     LVGSAWDTLLEGNVRHLIERDCLPAYLGRQRWFAGRSRTIRSARIVDWAVTRRGREPA
                     FLTITEVTFAGDQRERYLLPLATESGTQADRVLNETPETVLARLTGARKGVLYDACGG
                     EKIGRELLALMSGGGTLPTRHGVITGQRTSALDEVARECDLESLKASVVRREQSNSSL
                     IYGPALILKLIRRLDPGPNPELEIGRHLTERRGFTRAPALAGALSYQGPGDEPVTLGI
                     LHRLVEHQADGWGHAIEELGRYLERVTSEPWKAPELPGAAPALALSGAAPPARVADSI
                     GTYLETATRLGQRTAELHLALATPDGDPAFEPQPFTGRDLALTAARMAEEGRVTLDAI
                     EGALPGLPETAREAAARLVARRSDVVGALVSLSAVENPGRKIRVHGDFHLGQVLWAEG
                     DFYLLDFEGEPDRPLAERRSRQSPMKDVAGMLRSFGYAAEAALRAEPVVVGRQDGDLR
                     PWVAAWQTWVGAAFLRSYLAVAGEAPFLPGDRESLAAVLRVFLLEKALYELRYELNHR
                     PDWLPIPLSGIEEILGW"
     gene            complement(5468..7615)
                     /locus_tag="PKK95_07705"
     CDS             complement(5468..7615)
                     /locus_tag="PKK95_07705"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HOC18137.1"
                     /translation="MTRPPLPNDDRPRRAVVDRVRPEIDGGRFPIKRTTGERVEVIAD
                     VFVDGHDRLAVVLKYRLAEAPESPGIPHDRSWREVRMTPAGEDRWSASFMVDVLGRWE
                     YTVEAWVDRFASWRDELSKKYTAGLEVTSELLEGAWLVRQTAQRLATPLTGETPPHTG
                     ETPVPLSQPHTGETPVPLSQPHTGGTPVPLSQPHTGGTPVPLREWLIGRALLMEGPDE
                     ADLKVSAALADSLASAMAEHADRSLATRYHRVLSVTVERPRARFGAWYEFFPRSGRSP
                     ERSATFAEAAERLPEIAAMGFDVVYLPPIHPIGQSYRKGRNNSLVAAPGDPGSPWAIG
                     ADEGGHTAVHPELGGLPAFRDFVGAARRFNLEVALDIAFQASPDHPWVREHPEWFHHR
                     PDGTIKYAENPPKKYQDIYPFHFEAGGEAWRELWRELASVFFFWIEQGVRIFRVDNPH
                     TKPFRFWEWAIAEVRRHHPDAIFLAEAFTRPKPMCYLAKAGFSQSYSYFTWRNTKWEI
                     TSYFEALARGGVREYMRPNLFANTPDILPHYLQTGGRAAFIVRLVLAATLGASYGIYS
                     GFELCEHEAVPATEEYLDSEKYQIKPRDYDRPGHIKEIVARVNQARHANQALHADTSL
                     RFHETDNDQILCYSKVSPDRRNRILVVVSLDPHHPQQGFVRVPVSEMELAGLPSYTVR
                     DLLDEARYTWRHDWNFVRLDPASRPAHILRIEA"
     gene            complement(7587..9842)
                     /gene="glgX"
                     /locus_tag="PKK95_07710"
     CDS             complement(7587..9842)
                     /gene="glgX"
                     /locus_tag="PKK95_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOC18138.1"
                     /translation="MRVWPGSPYPLGATWNGMGVNFAIYCEHGTGVELCLFDHVRATR
                     ESARIKLVEQTDQVWHGFLPDVQPGQIYGYRIHGPYEPKNGHRFNPAKLVVDPYAKSI
                     ARPIQWSDEMFGYPIGHPDADLVRDDRDNAAFAPLAAVVDPAFVWGDDRPPATPLHRT
                     VIYETHVKGLTQLHPAVPPPFRGTYLGLASEPVLRHLLDLGVTAVELLPVHHHTPERS
                     LVERGLTDYWGYNTLAFFAPDIRYSASKEPLQSVREFKMMVRGLHAVGLEVILDVVYN
                     HTAEGNHLGPTLSLRGIDNLSYYRLVADNKRYYMDYTGCGNTLNMQSQRVLALIMDSL
                     RYWVTEMHVDGFRFDLASALARELHEVDQLGAFFDIIHQDPIISQVKLIAEPWDLGEG
                     GYQVGNFPVGWAEWNGKYRDSVRRFWRGDGGQVSEFATRLAGSSDLYERSGRRPYASI
                     NFITAHDGFTLQDLVSYNEKHNEANGEGNRDGANDNESWNCGVEGPTDDPAVVALRER
                     QKRNFFATLMLSQGVPMIRGGDELSQSQGGNNNAYCQDNDITWLDWKLTPQQIEFLDF
                     ARQVVHLRHEQPVLHRRRFFQGGRIRGADIKDITWFAPDGGEMADEAWTSSLARALGV
                     RLAGDAIDERDERGERITGDTLVLLLNAGDADVPFKLPPVAARCHWEVVFDSANGQEQ
                     AVPYDGGQEYEIQARSIAVLLLYREKRRRLSDRAQRLADLATLGVPARIRVTSAAPAK
                     VTDDTPAAPER"
BASE COUNT         1391 a         3310 c         3655 g         1602 t
ORIGIN      
        1 gtgctactgg gtgccacagg cgagacgcct gtgctactgg gtggggggct gggccagacg
       61 ccgagcagca cctggtagaa gcggtactcg tcgttgcggt cgggggccgg cctgccgttg
      121 atctcggtgc ggtggcccgc gttgatgcgt gcccaccgag agacgtgacg tctccagtcg
      181 ggtgccagct cggagatcac ggcgatgcgg gcgcgcacgt cctccccgag cttggtgtcg
      241 tgcgtggcgg tggtgagcat ctcctcgggc cagtggcgcg cgcgtgcctg gttgcgggcg
      301 tgcacctcct cgggtcggcg cccgaagcgc gagggctcgc cgcccacctc gttcagggcg
      361 accagaaggt tgtagcgata gaaggcggtg tcctcgacgc ccttggcctg gaccggcgcg
      421 gtgaactgct gcagcttcat cgagaagcgc aggcgcgcct ggtagccctc ctcgtccacc
      481 ggggcgtagc cgtcgcggcg gtcttcgaag gccgcttccg gcgagaggcg gcgcggcagg
      541 accgcctcgc ggaggaagtc gaactgcgag gcggggatgg tggggttgcg gcgcttcgcc
      601 acggccaccg cctcctcgac ccgggtgcgg tcggccggcg tcccgcctcc ctggtcgatg
      661 tacgtccggt agacggggaa gcaggcgacc acctcctgca gcacgcgccg gaggctctcg
      721 agggtgaagt cgcgcgaccg gcggttcgac tcggagatcc ggttgagggc gtgcgccagc
      781 acgttcagct cggaggcgag agacgcctgc gtgatcagct tcttgctgcg gtaggcgatc
      841 tcggcgaacg gccaggtgaa cccagtgaac cgcgcgtaga actgccgaag cggccgctcg
      901 ccggccggat cgatctgcag ccccgtgagg tcgttcgcga aggcgtagcc ggtggtgccc
      961 gcgatcggcc agtcgccggg cagcgtctcc tcgccgctga ggatcttctc ggcggccacc
     1021 cagagccgtc ggcggccgcc gcgcagttcc tgcagcttct cgaagtacgc cttcgggtcg
     1081 aagaggccgt cggggtggtc gatccgcagc ccgtccacct ggccgccgtc cagcagctcg
     1141 ccgacgagcg cgtgcgtcgc ctcgaagacc tcggggtcct ccatccggag cgccgccagg
     1201 gcattgatgt cgaagaagcg gcggtagttg atctcgtgcg aggcggagcg ccagtcggac
     1261 agccggtagg cctggcgctc gagcagctcg tgcaggggat cgaagctggc gggcgcgccg
     1321 ggcgtcccgt tgaagaaccg cacggcgcgt tcgacgtgcg agcggatctc ggcagacgag
     1381 gaaatcaggc gcccgaggcg ctcgcgggcc acttccttct cgcgccgccg ttcggcgatg
     1441 cgcgccgggt cggtctcctg gattgcgggg aggttgcgca gcgcggtcag gatgctgagg
     1501 aactcgcgca gggccgcgtg ctcctccccg agcgaggccg tcatctcctc gaggccgtgc
     1561 tcgtagacca tgacggcctg ccgcgggttc accggcagga ggttgtcgaa gtactcgagg
     1621 aagagcgcac cgtcctcgaa tccgaggcgc agctcgcccc gctcgagcac gagaccatac
     1681 tgatcgccca ggatcggcag cagcaccttg ccccgcagtt ccggcttgat cgggtcccag
     1741 tcgatgtcga agtacctcgc gaagggggac gactgaccgt tctcgagcac gtcgcgccac
     1801 caggggttcg tgcggggatc gatccccatg tggttcggga cgaagtcgat gatctggccg
     1861 aggccgtgat cgtgcagcgc ccggacgaac gcgtcgtagt cggccctgct gccgatctcc
     1921 gggttcagct cccggtggtt gcagatgtcg tagccgtggg tgctgcccgg cttcgccgtc
     1981 aggtagggcg acgagtagca gtgggtgacg ccgagcgcgg ccaggtacgg cgccagcgcg
     2041 gcggcatcgc ggaacgtgaa gcctccgtgg aactggaggc gataggtggc gagcggcgtg
     2101 tcgggcgggt tgggcatcgg ggcctcgcgt cagccgggtt tcaccacccg aggatttcct
     2161 cgattcccga cagcgggatc ggcagccagt cggggcggtg gttgagctcg tagcgcagct
     2221 cgtagagcgc cttctcgagc aggaacaccc gcagcaccgc cgcgagcgac tcgcggtcgc
     2281 cgggcaggaa cggcgcctcg ccggccaccg cgaggtacga ccgcaggaac gccgcgccga
     2341 cccacgtctg ccaggcggcg acccagggcc gcaagtcccc gtcctgcctg cccacgacaa
     2401 ccggttcggc gcgcagcgcg gcctcggcgg cgtagccgaa cgaccgcagc atcccggcaa
     2461 cgtccttcat cggcgactgc cgcgaccgcc gctcggccag ggggcggtcg ggttctccct
     2521 cgaagtcgag caggtagaag tcgccctcgg cccacaggac ctgacccagg tggaagtcgc
     2581 cgtgaaccct gatcttccgt cccgggttct cgaccgccga cagtgagacg agggcgccga
     2641 cgacgtcgga gcggcgggcc acgaggcggg cggcagcctc gcgcgccgtt tcgggaagcc
     2701 cgggcagcgc gccctcaatc gcgtccagcg tcacccggcc ctcctcggcc atccgggcgg
     2761 cggtcagggc gaggtcgcgc ccggtgaagg gctgcggctc gaacgccggg tcgccgtccg
     2821 gggtggccag cgcgaggtgg agttccgccg ttcgctggcc tagccgcgtt gccgtctcga
     2881 ggtaggtccc gatcgagtcg gcgacccggg cggggggggc ggcgccagag agggccagcg
     2941 ccggagcggc tcccggcagt tccggggcct tccatggctc gctggtgacg cgctcgaggt
     3001 agcgtcccag ttcctcgatc gcgtgccccc agccgtccgc ctggtgctcg accaggcggt
     3061 gcaggatgcc gagggtgacc ggttcgtccc ccggcccctg gtaggagagc gcgccggcca
     3121 gcgcgggagc gcgggtgaac ccgcgccgct cggtgaggtg ccggccgatc tccagttccg
     3181 ggtttgggcc ggggtcgagg cgccggatca gtttcaggat gagcgcgggg ccgtagatga
     3241 gcgagctgtt gctctgctcg cgccggacga ccgacgcctt gaggctttcg aggtcgcact
     3301 cgcgtgccac ctcgtcgagg gccgaggtgc gctgccccgt gatgacgccg tggcgcgtgg
     3361 gcagcgtgcc cccgccgctc atcagcgcca gcagttcgcg cccgatcttc tctcccccgc
     3421 aggcgtcgta cagcacccct ttgcgcgccc ccgtcagccg ggcgagcacg gtctcgggcg
     3481 tctcgttgag cacgcggtcg gcctgcgttc ccgactcggt ggccagcgga aggaggtagc
     3541 gctcgcgctg gtctcccgcg aaggtcacct cggtgatcgt gaggaacgcg ggctcgcggc
     3601 cgcggcgcgt caccgcccag tcgacaatgc gggccgagcg gatcgtgcgc gaccggccgg
     3661 cgaaccagcg ctgcctcccg aggtaggccg ggaggcagtc gcgctcgatc aggtggcgca
     3721 cgttgccctc gagcagcgtg tcccaggcgc tgccgacgag cagcgcgggg aactccgccg
     3781 ggctctctgc cggctgcggc acgacgcgcg cggagatcgg cgccggctgt tcgaccagct
     3841 cgaaccagta gaacgcgtac ggccccagcg tgaggaagta gggcagctcg ccgatgcgcg
     3901 ggaactcggt gcgcccgagc atctcgacgg gcgtgagccc cttgaactcc gacaggtcga
     3961 gctcgaccgg ctgcacgctg cgtgcgaggt tcgcgatgac gaagatgcgc ttcccgtcgc
     4021 cctcgcgcac gtaggccagc accttgcggt tggccgggtg aaggaaccgg gtcgcgccgc
     4081 ggccgaaggt gcggtgctgg cggcgcagcg ccagcatccg ccggaaccac tgcagcagcg
     4141 agtgcgggga gcgttcctgg gcctcgacgt tgatcgcctg gtagccgtag accgggtcca
     4201 tgatcggggg ggcgtagagg gccgccgggt cggcgcgcga gaacccggcg ttgcggtcgc
     4261 tcgtccactg catcggcgtc cggacgccgt tgcggtcgcc gaggtagatg ttgtcgccca
     4321 tccccagctc gtcgccgtag tagaggaccg gcgtgccggg cagcgagaag agcagcccgt
     4381 gcagcagttc gatccgccgc cggttgttct ccacgagcgg cgcgaggcgc cggcggatgc
     4441 ccaggttcag ccgcatccgc gtgtcggccg cgtaggcccg gtacatgtag tcgcgctcct
     4501 cgtcggtcac catctcgagg gtcagctcgt cgtggttccg gaggaagagg gcccactggc
     4561 acacctcggg gatgtcgggc gtctggtgga ggatctcggt gatcgggtgc cggtcctcct
     4621 gctggagcgc catgaacatc cgcggcatca gcgggaagtg gaaggccatg tggcactcgt
     4681 cgccgtcgcc gaagtacggc cgcacgtcgg cgggccactg gttggcctcg gcgagcagca
     4741 tccggtgcgg gtacttcgcg tcgacgtgcg cccggaactc cttgaggatc tgatgggtct
     4801 ccggcaggtt ctcgcagttg gtcccctcgc gctcgatcag gtagggcacc gcgtcgaggc
     4861 gcagggcgtc gacgcccatc tcgagccaga agtccatccg gccgcgcatg cggcgccgca
     4921 cctcggggtt gtcgtagttc aggtcgggct ggtggtggaa gaaacggtgc cagtagtacg
     4981 cgccggcgtc gaagtcccac gtccagttcg aggtctcggt gtcggtgaag atgatgcggg
     5041 cgtcctcgta gcgctgcttg gtgtcgctcc agacgtagaa ctcgcgctcg cgcgagccgc
     5101 gcggcatgcg acgggcgcgc ttgaaccacg ggtgctggtc ggagctgtgg ttcatcacca
     5161 gctcggtcag cacgtagagg ccgcggcggt gcgcctcgtc gaggaagcgc cggaagtcgc
     5221 gaagcgtccc gtatgccggg tgcaccccct ggtaccacga gatgtcgtac ccatcgtcac
     5281 ggagcggcga agggtagaag ggcagcagcc agatgcaggt gatgccgagc gcctccaggt
     5341 agtcgagctt cgaggccagg ccggcgaagt cgccgatgcc gtcgccgttg ctgtcgagga
     5401 acgccttgac gtgcacctcg tagatgacgg cgtccttgta ccagaggggt tcagccatgg
     5461 tgtccggtca cgcctcgatg cgcaggatgt gggccggcct ggaggcgggg tccagccgga
     5521 cgaagttcca gtcgtggcgc caggtgtagc gcgcctcgtc gaggaggtcg cggaccgtgt
     5581 acgacggcag gcccgccaac tccatctcgc tcaccggcac cctcacgaac ccctgctgcg
     5641 ggtggtgcgg gtcgagcgag accacgacga ggatccggtt gcggcggtcg ggtgacacct
     5701 tcgagtaaca gaggatctgg tcgttgtcgg tctcgtggaa ccgcagggac gtgtcggcgt
     5761 gcagcgcctg gttcgcgtgc cgcgcctggt tgacgcgcgc gacgatctcc ttgatgtgtc
     5821 ccgggcgatc gtagtcgcgc ggcttgatct ggtacttctc cgagtcgagg tactcctcgg
     5881 tcgccgggac ggcctcgtgc tcgcacagct cgaagccgct gtagatgccg tagctcgcgc
     5941 cgagggtcgc cgccagcacc aggcggacga tgaaggcggc gcggccgccc gtctgcaggt
     6001 agtggggcag gatgtcgggc gtgttcgcga acaggttcgg gcgcatgtac tcgcgcacgc
     6061 ccccccgggc cagcgcctcg aagtagctgg tgatctccca cttcgtgttg cgccaggtga
     6121 agtaggagta cgactgcgag aacccggcct tcgccaggta gcacatcggc ttggggcggg
     6181 tgaacgcctc ggcgaggaag atcgcgtcgg ggtggtgccg gcggacctcg gcgatggccc
     6241 actcccagaa gcggaacggc ttggtgtgcg ggttgtccac ccggaagatc cggacgccct
     6301 gctcgatcca gaagaagaac accgaggcca gctcgcgcca cagctcgcgc cacgcctccc
     6361 cgccggcctc gaagtggaac gggtagatgt cctggtactt cttgggcggg ttctccgcgt
     6421 acttgatcgt cccgtcgggg cggtgatgga accactcggg gtgctcgcgc acccagggat
     6481 gatcgggtga ggcctggaac gcgatgtcca gcgcgacctc gaggttgaac cggcgggccg
     6541 cgccgacgaa gtcgcggaac gccgggaggc cgcccagctc ggggtggacc gcggtgtgcc
     6601 ctccctcgtc tgcgccgatc gcccaggggc ttccagggtc gccgggcgcg gccacgaggc
     6661 tgttgttgcg ccccttccgg tagctttggc caatcgggtg gatcgggggc aggtacacca
     6721 cgtcgaaccc catcgccgcg atctcgggca gccgttcggc ggcctcggcg aaggttgcgc
     6781 tgcgttcggg cgaccgcccc gagcggggga agaactcgta ccaggcgccg aaccgcgccc
     6841 gcgggcgctc gacggtcacc gagagcaccc ggtggtagcg ggtcgccagc gaccggtcgg
     6901 cgtgctcggc catcgcggag gcgagcgagt cggcaagggc cgccgacacc ttcaggtcgg
     6961 cctcgtcggg cccttccatc agcagcgccc ggccgatgag ccactcgcgc agcggcacag
     7021 gcgtcccgcc tgtgtgtggc tggctcagtg gcacaggcgt cccgcctgtg tgtggctggc
     7081 tcagtggcac aggcgtctcg cctgtgtgtg gctggctcag tggcacaggc gtctcgcctg
     7141 tgtgtggtgg cgtctcgcct gtgagtggtg tggccagcct ctgggccgtc tgcctcacca
     7201 gccaggcccc ttcgagcagc tcgctggtca cctcgaggcc ggccgtgtac ttcttcgaca
     7261 gctcgtcgcg ccacgaggca aaccggtcca cccaggcctc gaccgtgtac tcccagcggc
     7321 cgaggacgtc caccatgaac gaggcgctcc accggtcttc gccggccggg gtcatccgca
     7381 cctcgcgcca cgatcggtcg tgggggatgc ctggcgactc gggcgcctcc gcgaggcggt
     7441 acttcagcac cacggcgagg cggtcgtggc catcgacgaa gacgtcggcg atcacctcga
     7501 cgcgctcgcc ggtggttcgc ttgatcggga accgtccgcc gtcgatttcc ggcctgaccc
     7561 gatcgacgac ggcgcggcgc gggcggtcat cgttcgggag cggcgggcgt gtcatcggtg
     7621 accttcgccg gtgcggctga ggtgacgcgg atgcgcgcgg gcacgccgag cgtggcgagg
     7681 tcggccagcc gctgcgcgcg gtcggacaac cggcggcgct tctcccggta gagcagcagt
     7741 accgcgatgg agcgagcctg gatctcgtat tcctgccccc catcgtaggg gaccgcctgt
     7801 tcctggccgt tcgccgagtc gaagacgacc tcccagtggc agcgcgcggc gacgggcggc
     7861 agcttgaacg ggacgtcggc gtcgcccgcg ttcagcagca ggaccagcgt gtcgccggta
     7921 atccgctcgc cccgctcgtc gcgctcgtcg atcgcgtcac cggccaggcg gacgccgagc
     7981 gcgcgcgcga ggctggaggt ccaggcctcg tcggccatct cgccgccgtc gggggcgaac
     8041 caggtgatgt ccttgatgtc ggcgcctcgg atccgcccgc cctggaagaa gcggcggcgg
     8101 tgcagcacgg gttgctcgtg ccggaggtgc acgacctggc gggcgaagtc gaggaactcg
     8161 atctgctgcg gggtcagctt ccagtcgagc caggtgatgt cgttgtcctg gcagtaggcg
     8221 ttgttgttgc cgccctggct ctggctcagc tcgtcgccgc cccggatcat cggcacgccc
     8281 tgcgacagca tgagcgtcgc gaagaagttg cgcttctgcc gctcgcgcag cgcgacgacc
     8341 gccgggtcgt cggtcggccc ctcgacgccg cagttccagc tctcgttgtc gttggcgccg
     8401 tcgcggttcc cctcgccgtt cgcctcgttg tgcttctcgt tgtagctgac caggtcctgc
     8461 agcgtgaagc cgtcgtgggc cgtgatgaag ttgatgctcg cgtacgggcg gcgcccgctg
     8521 cgctcgtaga ggtcgctgct gccggcaagg cgcgtcgcga actcggacac ctgcccgccg
     8581 tcgccccgcc agaagcgccg caccgagtcg cggtacttgc cgttccactc ggcccagccg
     8641 acagggaagt tcccgacctg gtatccccct tcgccgaggt cccacggctc ggcaatgagc
     8701 ttcacctgcg agatgatcgg gtcctggtgg atgatgtcga agaaggcgcc cagctgatcc
     8761 acctcgtgca gctcgcgggc cagggcgctc gccaggtcga agcggaaccc gtccacgtgc
     8821 atctcggtga cccagtagcg caggctgtcc atgatcagcg cgagcacgcg ctggctctgc
     8881 atgttcaggg tgttcccgca ccccgtgtag tccatgtagt agcgcttgtt gtcggcgacc
     8941 aggcggtagt aggagaggtt gtcgatgccc cgcagcgaga gcgtcgggcc gagatggttc
     9001 ccctcggccg tgtggttgta gaccacgtcg aggatgacct cgaggccgac cgcgtgcagc
     9061 ccgcgcacca tcatcttgaa ctcgcgcacc gactgcagcg gctccttcga ggccgagtag
     9121 cggatgtcgg gcgcgaagaa ggccagcgtg ttgtatcccc agtagtcggt caggcctcgc
     9181 tcgaccaggc ttcgctccgg ggtgtggtga tgcaccggca gcagctcgac cgccgtgacg
     9241 ccgaggtcga gcaggtgccg caggacgggc tcggaggcga gcccgaggta ggtgccgcgg
     9301 aacggcgggg gcacggccgg gtgcagctgg gtcagcccct tgacgtgcgt ttcgtagatg
     9361 accgttcggt gcaggggagt ggccggcggg cggtcgtccc cccagacgaa cgcggggtcc
     9421 accaccgccg ccagcggggc gaacgcggcg ttgtcgcgat cgtcgcgcac gaggtcggcg
     9481 tcggggtggc cgatggggta gccgaacatc tcgtccgacc actggatcgg ccgcgcgatg
     9541 ctcttcgcgt acggatcgac gacgagcttc gcggggttga agcggtggcc gttcttcggc
     9601 tcgtagggcc cgtggatccg gtagccgtag atttgcccgg gctgcacgtc tggcaggaac
     9661 ccatgccaga cctggtcggt ctgctcgacc agcttgatgc gcgccgactc cctggttgcc
     9721 cgcacatggt cgaacaggca cagctcgaca cccgtgccgt gttcgcagta gatggcgaag
     9781 ttgaccccca tcccgttcca cgtggccccc agggggtagg gggatcccgg ccagacgcgc
     9841 atgccggcac ctccgcccgg ccttccaaag catgaggtat gccgtacagg accgccgatg
     9901 agcggctatt tcgagaagtc gacctttggc tcctgtttcg cctcagcggg cgcctcga
//
LOCUS       DAOPQX010000110         9937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7736_length_9937_cov_4.108278, whole genome shotgun sequence.
ACCESSION   DAOPQX010000110 DAOPQX010000000
VERSION     DAOPQX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7736_length_9937_cov_4.108278"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..459
                     /locus_tag="PKK95_07715"
                     /pseudo
     CDS             43..459
                     /locus_tag="PKK95_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014793.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
     gene            complement(610..1257)
                     /locus_tag="PKK95_07720"
                     /pseudo
     CDS             complement(610..1257)
                     /locus_tag="PKK95_07720"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(1155..1511)
                     /gene="ndhC"
                     /locus_tag="PKK95_07725"
     CDS             complement(1155..1511)
                     /gene="ndhC"
                     /locus_tag="PKK95_07725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOC18139.1"
                     /translation="MPAWSLPLYLAAVVFVVATMIGLAAVLGERRRHRVAIPYESGML
                     PTGSARVRFPARFYLVAVFFVVFDLEAVFLFAWALAARELGWNGYWAMVVFVAILGVT
                     LAYLWRSGALDWNEGR"
     gene            1671..3581
                     /locus_tag="PKK95_07730"
     CDS             1671..3581
                     /locus_tag="PKK95_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOC18140.1"
                     /translation="MSATRYDERPDFTRPPLELDPGLRDRIAAHLRALYGEEATSDIL
                     PELTRLLKVHHAHKPAALVEAGLAFDPSRRFSERDMGLVVYGDMIRRDGRVPLAALDD
                     LLAEFLGRRTFNILHVLPFFPYSSDRGFSVTDFLSVDPRLGSWQDIERLAGSFKLMFD
                     AVLNHVSSESTAFREMLAGAPGFKDLAVVFRSPEEVSEDQRRLLRRPRTSEILTRFQA
                     LDGPIWAWTTFSPDQVDLNYRNPRVLLMVIEALLMYVRRGASMIRLDAITYLWDEPGT
                     TGASLAQTHEVVRLLRAVLDASAPEVALVTESNVPHDENVSYFGNGLDEAQAVYNFAL
                     PPLVLHAFYRGDASWLTDWAERLTYPSLAATYLNLLDSHDGIGLPGISGILPAGEVSF
                     LVDRAREHGAFVSFGAASEGVRSPYELNTTWYSALNLDNSDEERAFQVKRFVASRSIA
                     LALAGVPVIYLHGLIGSRSDVQLALRTGSRRDVNRSSVDPVMVRQNLADRGSKLSMIR
                     ENLGRLLQVRVRQRAFHPNGGQSVLRLARQVFALRRTSPVGDEHVLALTNVSGQPCVV
                     EVPLEQAGVAAANWYDFVSGRGWSARGGSLTLALGPYDVLWLKPFQEIEREIERHDPR
                     AGSGALAGGLPG"
     gene            3670..4368
                     /locus_tag="PKK95_07735"
     CDS             3670..4368
                     /locus_tag="PKK95_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOC18141.1"
                     /translation="MRRLVTVSVLLLVSVAVGSPAAQEKERERLEQCGVVMEEILGVP
                     DNIPQELLDKAECVIVIPSMVKGAIGVGYSRGRGAMVCRSGKAFDGAWGAPAMYALDG
                     GSFGFQLGGQATDLVLLVMNPRGVNALLGSKVKLGANASVAAGPKGRDAAAATDATMR
                     AEILSYSRSRGLFAGLSLEGTSVRPDNDASRKVYGRKLGARAIVQGDGVEVPEPGRRL
                     VNLLDKVSPRNLSK"
     gene            complement(4375..5265)
                     /locus_tag="PKK95_07740"
     CDS             complement(4375..5265)
                     /locus_tag="PKK95_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC18142.1"
                     /translation="MTKLAVRLSAPVVVALLWLSPSPRGAEADRAQAADQQPLALRVM
                     TYNLRYASDTPPNSWPERRPLVKKTVLQAAPDVIGTQEGVYRQLQDLAADLSAYHWIG
                     LGREGGSRGEFMAVFYRHARFVPVAFDHFWLSDTPEVIGSVTWGHTVRRMLTWVRLRD
                     KTTGRELLVWNTHLDHQVEDARRKGAALIRERLARTDPGVPLVLTGDFNCPAAGCVAY
                     ETLTREAGLTDAWLLAERRENEGIDTFNGFKAPGKGGVRIDWILLRGPAAVSRAATLT
                     DSGGGRFPSDHFPVVADVRF"
     gene            complement(5428..5880)
                     /locus_tag="PKK95_07745"
     CDS             complement(5428..5880)
                     /locus_tag="PKK95_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOC18143.1"
                     /translation="MFGRMRWSPFEELFNFQREVDRLFSRFWSDLPARTESFPAGSFQ
                     VKSNDQGWRVEVPMAGIDPKHVTLEVAGNNLNIKAEVPGDEKDDRDAVRFEQSLTLPQ
                     FLDLDKLTATHRHGMLELQVPLKDSVKPRKIEIRQLGAGEERKQLASV"
     gene            complement(6088..6609)
                     /locus_tag="PKK95_07750"
     CDS             complement(6088..6609)
                     /locus_tag="PKK95_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOC18144.1"
                     /translation="MGPARLTATQLTAWFESKTGAAGAYRASVPVETLARQFIDEGAA
                     EGVAGDIAFVQAIVETGWFRFPGLVSPAQNNFGGICATDHHPAPATFPDARTGVRAQI
                     QHLRAYADPGAVACAIPPLRHPCADPRFHLVVPKGKARSWNEMGGGNWAASPEYGQTI
                     LGLYREALAVAGR"
     gene            complement(6794..7426)
                     /gene="frr"
                     /locus_tag="PKK95_07755"
     CDS             complement(6794..7426)
                     /gene="frr"
                     /locus_tag="PKK95_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOC18145.1"
                     /translation="MDPWSWPGETGVPCRPGVEQHMDVNDLKSLNAEAKKRMAAAIEH
                     LRRDLAGVRTGRASTGILEPVQVEAYGTRVPLNQVASLSVPEPTMIIAQPFDPTLMGA
                     IERAVRASDLGLNPNNDGKVVRIPVPALTDERRRELSRHVHKLSEECRNAVRAVRREA
                     NERLKKLAKDRKVSEDEEKRGLDEVQKLTDQHIAQIDELQKKKDAELLTR"
     gene            complement(7401..8129)
                     /gene="pyrH"
                     /locus_tag="PKK95_07760"
     CDS             complement(7401..8129)
                     /gene="pyrH"
                     /locus_tag="PKK95_07760"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOC18146.1"
                     /translation="MDTSAPPAYRRILLKLSGEALMGEQKFGIDPEVAAQIARDVAGV
                     RRLGVEVALVIGGGNIFRGVAASARGMDRATADYMGMLATCINALALQDALEHEGVVT
                     RTLTAIEMRAVAEPFIRRRAVRHLEKGRVVIFAAGTGNPYFTTDTAAALRAMEIRADV
                     IFKATKVDGIYTADPVKHPDAERFDRITYLQVLEKGLQVMDATAISLCMDNHMPIVVF
                     NLRTPGNLERAVRGESVGSLVMAG"
     gene            complement(8416..9018)
                     /gene="tsf"
                     /locus_tag="PKK95_07765"
     CDS             complement(8416..9018)
                     /gene="tsf"
                     /locus_tag="PKK95_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOC18147.1"
                     /translation="MAITAEQVKTLRERTGAGMMECKAALIETGGNLEEAITVLRKRG
                     LAQAAKRSGRATRQGMVGSYIHLGGKVGVLVEVNCESDFVARTEAFQTLVKEIAMQIA
                     AADPRYVSRAQVPAAELEKEKEIVRAQFAGSNKPANVIEKIVEGKLDAFYERTVLVDQ
                     PSIRDGAVKVSQMIAAAVAQTGENITVSRFARFKLGEASE"
     gene            complement(9136..9936)
                     /gene="rpsB"
                     /locus_tag="PKK95_07770"
     CDS             complement(9136..9936)
                     /gene="rpsB"
                     /locus_tag="PKK95_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003319565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOC18148.1"
                     /translation="MAGIALKELLEAGVHFGHQTKRWNPKMKPYIFGERNGIYIIDLS
                     KTAKQFREAEEFVSRIASEGGHILFVGTKRQAQDAIAEEAQRAGMHFVNQRWLGGLLT
                     NFTTIQRSLGRLRELEAMATDGRYETLSKKEIARIEKEKRKLQKNLDGIRQMGRLPDA
                     VFVVDTHKEKIAVDEARKLKIPVIGVVDTNSDPDEVDYVIPGNDDALRAIRLFASRLA
                     DAIVAGRGLREAAQAEVSEEATEARAARGGRRPRPDHAPVPPASPASA"
BASE COUNT         1504 a         3351 c         3495 g         1587 t
ORIGIN      
        1 ccggggctga tcgccaaccc gaattgttcg acgatccagc tggtggtcgc gctggagccg
       61 atccggcggc ggtatggact gcgcagcgtc tttgtcgcca cctaccagtc cgcctcgggg
      121 aagggatacg ccggcgtact ggcgctgcgg gcggagcgcg aagcggcatc ggccgcgggc
      181 gctccggcga acgtgagtcc ggagagttcc ggcgccgtgc cggcggcgtc gcacgtcgcc
      241 tcggcgttgc cacacgccgg tcaagccccg gtgcccgctc cgccggtctt cccgtgcccg
      301 ctggacggca atgtcatccc gcactgcgat gtcttccttc ccgacggctt cacccgcgag
      361 gaggagaaga tgatccagga gacccgcaag atcctggaca tgccggcgct ggcggtccat
      421 cccacctgcg tgcgcgtgcc ggtggaagtc gcgcactccg aggccgtgta cctgcagacg
      481 gaggaacccg tctcggcgga ggaagcgcgc ctgctggcgc tcggcacgct tgaggtcgcg
      541 catcgacggc atcggctggc gttcgacgac ctgggacccc accgcccagc tcagcgggcg
      601 gcgctcggat ccaaccgcct tctggagcaa gagcaggccc tccatcaggc tgtctgggcg
      661 cggggggcag cccgggacgt agacgtccac cgggaggaac ttgtccaccc cctggaccac
      721 gctgtacacg tcgtacatcc cccccgagtt ggcgcacgac cccatcgaga tgacccagcg
      781 cggttcgcgc atctgctggt agaggtgctt gatcaccggc gccatcttga ggaagaccgt
      841 gccggccacc acgagcacgt cggcctcgcg cgggctggcg cgaatcacct ccgagccgaa
      901 gcgcgcgagg tcccagcggc tggtgaggct ggtcgccatc tccacgtagc agcaggagag
      961 gccgaagttg aacggccaca gggagttctt gcggccccac gcgaccagct cgtcgagccg
     1021 cgcgagcacc accgaccgcc gcgtcgcctc ctcgaacgat tccctgtcat cgaggccctc
     1081 gcccggagcg cccctcccaa ccccgggagc gccctccgcg cccgaccccg gccggctcag
     1141 ccaccagcgc acccttacct cccttcgttc cagtcgaggg ccccggagcg ccagagatac
     1201 gcgagggtca ccccgagaat cgcgacgaag acgaccatcg cccagtagcc gttccatccc
     1261 agctcgcggg ccgcgagcgc ccatgcgaac aggaacacgg cctcgaggtc gaagacgacg
     1321 aagaagaccg ccacgaggta gaaccgggcc gggaagcgca cgcgcgccga acccgtcggc
     1381 agcatccccg actcgtatgg aattgcgaca cggtgccgtc ggcgctcgcc gagcaccgct
     1441 gccaggccaa tcatcgtggc gacgacgaag acgacggcgg cgaggtagag cggcagggac
     1501 cacgcaggca tgaatcctcg ccagccggcc tagcggccag ccttgctggt gtgtcgtccg
     1561 aggtcggagc ccggcccgga atcgagcaag agccgttcca cgacggattc gtccgcgatg
     1621 ggtttggctc gaaggaacgg ctcgggcggg cgctggggta ggatttccgc atgagcgcca
     1681 cccgatacga cgagcggccc gacttcacgc gccctcccct cgagctcgac ccggggctcc
     1741 gcgaccgcat cgcggcgcac ctccgagccc tctacggtga ggaggccacc tccgacatcc
     1801 tgccggagct cacgcgcctg ctgaaagtgc accatgctca caagccggcc gcactcgtgg
     1861 aggcgggcct ggcgttcgac ccgtcccggc gcttcagcga gcgggacatg ggcctggtcg
     1921 tctacggcga catgatccgg cgcgacgggc gggtgccgct ggcggcgctc gacgatctcc
     1981 tcgcggagtt cctggggcgg cgcaccttca acatcctcca cgtgctgccg ttcttcccct
     2041 acagcagcga tcgcggcttc tcggtcaccg atttcctgag cgtcgacccc cggctcggct
     2101 cctggcagga catcgagcgg ctggccggct cgttcaagct catgttcgac gcggtgctca
     2161 accacgtctc gtcggagagc acggcgttcc gcgagatgct ggccggcgcc cccggcttca
     2221 aagacctcgc cgtcgtcttc cgctcgcccg aggaggtgtc ggaggatcag cggcggctgc
     2281 tgcggcggcc gcggacctcg gagatcctca cgcgcttcca agcgctcgac ggtcccatct
     2341 gggcctggac gacgttctcg cccgaccagg tcgatctcaa ctaccgcaac ccgcgggtgc
     2401 tgctgatggt gatcgaggcg ctgctgatgt acgtgcggcg cggcgcgtcg atgatccggc
     2461 tcgacgccat cacctacctg tgggacgagc cgggtaccac cggcgcgagc ctcgcccaga
     2521 ctcacgaggt cgtgcggctg ctgcgggcgg tgctcgacgc gtcggcgccg gaggtcgcgc
     2581 tggtgaccga gagcaacgtg ccgcacgacg agaacgtctc gtacttcggc aacgggctcg
     2641 acgaggcgca ggcggtctac aacttcgcgc tcccgccgct cgtcctgcac gccttctacc
     2701 gcggcgacgc gtcgtggctc accgattggg ccgagcgcct cacttaccca tcgctggccg
     2761 ccacttattt gaacctgctc gactctcacg acgggatcgg tctgccgggc atcagcggca
     2821 tcctgcctgc cggcgaagtg tcgttcctcg tggaccgcgc gcgcgagcac ggggcgttcg
     2881 tgtcgttcgg cgccgcctcc gagggtgtgc ggtcgcccta cgaactcaac acgacgtggt
     2941 acagcgcgct caacctggac aacagcgacg aggagcgggc cttccaggtg aagcgcttcg
     3001 tcgcatcccg gtcgattgcg ctcgcgctgg cgggggttcc ggtcatctac ctgcacggcc
     3061 tcatcggcag ccgcagcgac gtccagctgg ccctgcggac gggttccagg cgcgacgtca
     3121 accgctcgtc ggtggatccg gtcatggtgc gccagaacct ggccgaccgc ggatcgaagc
     3181 tgtcgatgat ccgcgagaac ctcggacggc tgctccaggt gcgcgttcgc caacgcgcgt
     3241 tccacccgaa cggcggccag tcggtgctgc gcctggcgcg gcaggtgttc gcgctgcggc
     3301 gcacctcgcc cgtcggcgac gagcacgtgc tcgcgctcac caacgtgagc ggccagccgt
     3361 gcgtggtgga ggtgcctctc gaacaggccg gggtggcggc ggccaactgg tacgacttcg
     3421 tctcgggacg ggggtggagc gcgaggggag gaagtctgac gctcgccctc gggccgtacg
     3481 acgtgctgtg gctcaagccg ttccaggaga tcgagcggga gatcgagcgg cacgatccgc
     3541 gcgccggatc cggggcgctg gccggcggcc tgccaggata gacagacgac cggatctgtc
     3601 gatccaacgc aggcattgga gtagtctggt ctcacgcggc tcgtccgcgc cttcctgcga
     3661 ggtgtccgta tgcgtcgcct tgtgaccgtc tcggttctgc tgctcgtgtc ggttgccgtg
     3721 ggcagccccg ccgcccagga gaaggagcgt gagcgcctgg agcagtgcgg cgtggtgatg
     3781 gaggagatcc tcggggtgcc cgacaacatt ccccaggagc tgctcgacaa ggcggagtgc
     3841 gtgatcgtga tcccgtcgat ggtgaagggg gccatcggcg tgggatacag ccgcgggcga
     3901 ggtgccatgg tgtgccggtc gggcaaggcg ttcgacgggg cctggggggc tcccgccatg
     3961 tacgcgctcg acgggggctc gttcggcttc cagctgggcg ggcaggccac cgacctggtg
     4021 ctgctcgtga tgaacccgcg cggcgtcaac gcgctgctcg ggagcaaggt gaagctgggc
     4081 gcgaacgcgt cggtcgccgc cgggccgaag gggcgcgacg cggccgcggc caccgacgcc
     4141 accatgcggg ccgagatcct cagctactcg cgatcgcggg gcctcttcgc gggcctctcg
     4201 ctcgagggca cctcggtgcg ccccgacaac gatgcaagcc ggaaggtgta cgggcggaag
     4261 ctgggcgcgc gcgccatcgt ccagggtgac ggcgtggagg tgcccgagcc gggccgccgc
     4321 ctcgtcaacc tgctcgacaa ggtgtcgccc cgcaacctgt cgaagtagac gggctcagaa
     4381 gcgcacgtcg gcgacgacgg ggaagtggtc gctcgggaaa cgcccgcccc ccgagtcggt
     4441 gagggtcgcg gcgcggctca ccgccgcggg gccgcgcagc aggatccagt cgatccgcac
     4501 gccgcctttg ccgggcgcct tgaacccgtt gaaggtgtcg atcccctcgt tctcgcgccg
     4561 ctcggcgagc agccacgcgt cggtgagccc cgcctccctg gtcagcgtct cgtaggcgac
     4621 gcagccggcg gcggggcagt tgaagtcgcc ggtgagcacg agcgggaccc ccgggtcggt
     4681 cctcgcgaga cgctcgcgga tcaacgcggc gcccttgcgg cgcgcgtcct cgacctggtg
     4741 atccaggtgc gtgttccaca ccagcagttc gcgcccggtc gtcttgtcgc gaagcctgac
     4801 ccaggtgagc atcctgcgga ccgtgtggcc ccacgtgacc gagccgatga cctcgggggt
     4861 gtccgacagc cagaagtggt cgaacgcgac cggtacgaaa cgggcgtggc ggtagaagac
     4921 cgccatgaac tcgccacggc ttccgccctc ccttcccagg ccgatccagt ggtaggccga
     4981 caggtcggct gcaaggtcct gcaactgccg gtagacccct tcctgggtgc cgatgacgtc
     5041 gggcgccgcc tggagaaccg tcttcttgac gagcgggcgc cgctcgggcc acgagttggg
     5101 tggcgtgtcg ctggcgtatc gaaggttgta ggtcatcacg cgcagcgcga gaggctgctg
     5161 gtcagctgcc tgcgctcgat cggcttcggc accgcgagga gaaggcgaca gccagaggag
     5221 ggcgaccacg acgggggcgg acagccgcac ggcaagcttc gtcataccgg ggttatagca
     5281 cgggtgtctc gagcggggtg gacggacggc tcccgaggat ccgtgtcagg aggcggcgta
     5341 caaacgacgg gccggccttc cggcggcgat cgagccaacc gggggaagac cggccccgcg
     5401 acaatcgcga gctgcgcccg gcagcgatca cacggaagcg agctgcttgc gttcctcgcc
     5461 cgcgccgagc tgccggatct cgatcttccg gggcttcacg ctgtccttca gcggaacctg
     5521 cagctcgagc atgccgtgcc gatgggtcgc cgtcagcttg tccaggtcga ggaactgcgg
     5581 cagcgtgagc gactgctcga accgcacggc gtcgcggtcg tccttctcat ctccggggac
     5641 ctccgccttg atgttcaggt tgttccccgc gacctcgagg gtgacgtgct tcggatcgat
     5701 gccagccatg ggcacctcaa cgcgccaccc ctggtcgttg ctcttcacct ggaacgaacc
     5761 ggccgggaaa ctctcggtcc gggcggggag gtcgctccag aaacggctga acagccggtc
     5821 aacctcccgc tggaaattga acagctcctc gaacgggctc caacgcatcc gtccaaacat
     5881 ctgatgaacc tcctttccgg tgagactctg ttgagcttct ttctacccgc tttggcggcg
     5941 cttgctttct cgttctcggc cgccagcacc aacaaggata gaacatgatt gtaagaatgt
     6001 caatagatat gagtgtctaa cagtcatatt gttgggccag caaggctctt gtctctccgc
     6061 ccggcgacca tatggcaagg gaagaggtca gcggccggcc acggcgagcg cctcgcgata
     6121 cagccccagg atcgtctggc cgtattccgg cgaggccgcc cagtttccac cgcccatttc
     6181 gttccaggac cgcgccttcc ccttggggac cacgaggtgg aaacgagggt cggcgcacgg
     6241 gtgtcgcagg ggagggatcg cgcacgcgac cgcgccggga tcggcgtacg cgcgcaggtg
     6301 ctggatctgg gcgcggacgc cggtgcgcgc gtcggggaag gttgcgggcg cggggtggtg
     6361 gtcggtcgcg cagatgcccc cgaaattgtt ctgcgcgggc gagacgaggc cgggaaagcg
     6421 gaaccacccg gtttcgacga tggcctggac gaaggcgatg tcgccggcga ccccctcggc
     6481 ggccccctcg tcgatgaact gccgcgcgag cgtctcgacg gggacgctgg cccggtacgc
     6541 cccggcggcc ccggttttcg actcgaacca ggcggtgagc tgcgtcgccg tgaggcgggc
     6601 gggtcccatg atcgcgatat cggggaggcg ggcgacaggg ggaggagagg tgggtgggcg
     6661 gcgggcgcgg ccggcgcacg cgccgcccgc cagcgcgagg gacagggcga gcaggacgcc
     6721 caaaacgcgc cctggattca gaaggctgaa ggacgaatgt aagaacacgc caacggcctg
     6781 ctcgcccggg cccctacctg gtgagcagct ccgcgtcctt cttcttctgc agctcgtcga
     6841 tctgggcgat gtgctggtcg gtcagcttct ggacctcgtc gaggccccgc ttctcttcgt
     6901 cctccgacac cttccggtcc ttggcgagct tcttcaggcg ctcgttggcc tcgcggcgga
     6961 cggcgcggac ggcgttgcgg cactcttccg acagcttgtg gacgtggcgc gacagctcgc
     7021 ggcgccgctc gtcggtgagc gcggggacgg ggatgcggac caccttgccg tcgttgttcg
     7081 ggttcagccc caggtcggag gcgcggacgg cgcgctcgat ggcccccatg agggtcgggt
     7141 cgaacggctg cgcgatgatc atcgtcggtt cgggcaccga caacgacgcg acctggttga
     7201 gggggacgcg cgtgccgtac gcctcgacct gtacgggctc gaggatgccc gtcgaggcgc
     7261 ggcccgtgcg cacgcccgcc aggtcgcgcc ggaggtgttc gatggcggcc gccattcgct
     7321 tcttcgcttc cgcgttcagg ctcttgaggt cgttgacgtc catgtgctgc tccacgccgg
     7381 gccggcaggg aacgccggtt tcacccggcc atgaccaggg atccaaccga ttctcctcgc
     7441 accgcccgtt cgaggttgcc cggcgtcctc aaattgaaga cgacgatcgg catgtgattg
     7501 tccatgcaga gggagatcgc agtggcgtcc atgacctgga ggcccttctc gaggacctgc
     7561 aggtaggtga tgcgatcgaa gcgctcggcg tcggggtgct tcacggggtc ggccgtgtag
     7621 atgccgtcca ccttggtggc cttgaagatc acgtccgcgc ggatttccat cgcgcgcagg
     7681 gccgcggccg tgtcggtggt gaagtagggg ttgccggtac cggcggcgaa gatcaccacc
     7741 cgccccttct cgaggtggcg gacggcgcgc cgccggatga agggctcggc gacggcgcgc
     7801 atctcgatcg cggtcagcgt gcgggtgacc acgccctcgt gctcgagggc gtcctgcagc
     7861 gcgagcgcgt tgatgcaggt ggcgagcatg cccatgtagt cggcggtggc gcggtccatc
     7921 ccgcgggcgc tcgccgccac gcccctgaag atgttgccgc cgccgatgac gagcgccacc
     7981 tcgacgccca gccggcgcac gccggcgacg tctcgggcga tctgcgccgc cacctcgggg
     8041 tcgatgccga acttctgctc gcccatcagg gcctcgccgg agagcttgag aaggatgcgg
     8101 cggtaggcgg gcggagcgga ggtatccata cagaagggga ttataaaaca ggggatcagg
     8161 cgacggggat aggagagaac ggaaaacggc gaacgggaag acggaagacg ggaacgaaaa
     8221 cgcgaatgcg gaatcgagga tcgcagaaat cgggagcggg caatgaagag cgagaacggg
     8281 agcggcgagg agggaaaggg gaatgaagac tggggaaagg gaagagcgga atgcagaatg
     8341 tagagagacg gcccgggccg gcactacaca ttctgcattc tatgggttcc gtcctacagc
     8401 ctgcattcca ctccgctact ccgacgcctc gcccagcttg aagcgggcga aacgcgagac
     8461 ggtgatgttc tcgccggtct gcgccacggc ggcggcgatc atctgcgaca ccttcacggc
     8521 cccgtcgcgg atcgacggct ggtccacgag caccgtccgc tcgtagaagg cgtcgagctt
     8581 cccctcgacg atcttctcga tcacgttggc cggcttgttc gacccggcga actgggcgcg
     8641 gacgatctcc ttctccttct cgagctcggc ggccggcacc tgcgcgcggc tgacgtaccg
     8701 gggatcggcc gcggcgatct gcatcgcgat ctccttgacc agcgtctgga aggcctcggt
     8761 acgggcgacg aagtccgact cgcagttgac ctcgacgagc acgcccacct tgccgccgag
     8821 gtggatgtag ctgccgacca tgccctggcg agtggcgcgg cccgagcgct tggcggcctg
     8881 ggcgagcccc cgcttgcgca ggacggtgat cgcctcctcg aggttgccgc cggtctcgat
     8941 cagcgcggcc ttgcactcca tcatccccgc gccggtccgc tcgcgcaggg tcttgacctg
     9001 ttcagccgtg attgccatgg tgcttccttg ctccgttcgt tcggggcgcc gcgggcctgc
     9061 tcgggcccgg tacgcagaaa cgccggccgg ttgtggtgcc cccggccggc gggtagtggc
     9121 ctcgggtgcc gggactcagg cggacgccgg ggacgcggga ggcaccggag cgtggtcggg
     9181 ccgcgggcgg cggccgccgc gggcggcgcg cgcctcggtg gcctcctccg agacctcggc
     9241 ctgggcggcc tcgcgcagac cgcggccggc gacgatcgcg tcggccagcc gggaggcgaa
     9301 gagccggatc gcccggaggg cgtcgtcgtt gcccgggatc acgtaatcga cttcgtccgg
     9361 gtcgctgttg gtgtcgacca cgccgatgac cgggatcttg agcttgcgcg cctcgtcgac
     9421 cgcgatcttc tccttgtggg tgtccacgac gaagaccgcg tcggggaggc ggcccatctg
     9481 gcggatgccg tccaggttct tctggagctt gcgcttctcc ttctcgatcc gcgcgatctc
     9541 cttcttggac agcgtctcgt agcgcccgtc ggtggccatc gcctcgagct cgcgcagccg
     9601 cccgaggctg cgctggatcg tggtgaagtt ggtcagcagg ccgcccagcc agcgctggtt
     9661 gacgaagtgc atccccgcgc gctgcgcctc ctcggcgatc gcgtcctggg cctggcgctt
     9721 ggtcccgacg aagaggatgt ggccgccttc cgaggcgatg cgggagacga actcctcggc
     9781 ctcgcggaac tgcttggccg tcttcgacag gtcgatgatg tagatgccgt tccgttcgcc
     9841 gaagatgtac ggcttcatct tcggattcca gcgcttcgtc tggtgcccga agtgaacgcc
     9901 cgcctccagc agctctttca acgcgatccc ggccaag
//
LOCUS       DAOPQX010000111         9913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7752_length_9913_cov_4.693751, whole genome shotgun sequence.
ACCESSION   DAOPQX010000111 DAOPQX010000000
VERSION     DAOPQX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7752_length_9913_cov_4.693751"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2282)
                     /gene="metH"
                     /locus_tag="PKK95_07775"
     CDS             complement(<1..2282)
                     /gene="metH"
                     /locus_tag="PKK95_07775"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOC18149.1"
                     /translation="MSPTLTDLLAERILLLDGAMGTMVQRHGLDEAGFRGDRFRDHPR
                     DLRGNTDVLVLTQPDLIRGIHEEYLAAGADLVETCTFTATSVAQADYGCERFVYEMNV
                     AAARLARQAADAWTARTPGRPRLVAGAVGPTNRTLSLSPDVADPAFRAITFDQLRAAY
                     LEQVRGLVDGGCDALLIETIFDTLNAKAAIAAAEDLFEERGRRLPLMISVTITDRSGR
                     TLSGQTVDAFWVSVAHARPVTVGINCALGAREMRPYLAELSRIAATRISCYPNAGLPN
                     AFGGYDETPEQTAAAIREFAEAGLVNLVGGCCGTTPAHIAAIATAIEGLAPRAVPDLP
                     RFTQYAGLETLTIRPDSNFQMIGERTNITGSKRFRRLVTSGDFAAAADVALEQVRGGA
                     NILDVNMDEAMLDSEAAMTRFLNLIATEPEIARLPVMIDSSRWPVVEAGLKCVQGKAI
                     VNSISLKEGEAEFLRQARLARRYGAAVVVMAFDERGQADTAARKVEVLSRAYRLLTAE
                     AGFDPADIVFDPAVLAVGTGIEEHDTYAVAFIEAVRELKARFPEAKISGGISNLSFSF
                     RGNDPVREAIHSAFLYHAIRAGLDMGIVNAGQLVVYEDIPPELLERVEDLLFNRRPDA
                     TERLVQAAGSPGPARREQAEAEWRKGTVQQRLSHALVHGILDHLEADIAEALALYPRP
                     LDIIEGPLMDGMKVVGDLFGSGRMFLPQVVKSARAMKKAVSFLEPLMAAESAARPSQG
                     RIVLATVKGDVHDIGKNIVGV"
     gene            2311..2799
                     /locus_tag="PKK95_07780"
     CDS             2311..2799
                     /locus_tag="PKK95_07780"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HOC18150.1"
                     /translation="MPIRVLITGGTFDKEYDEINARLVFKESHLPEMLRLGRCRLDLN
                     VRSIMMIDSLDMTEADREVVVQNCRQCEETRIVVTHGTDTMVETAAELARGVPGKTIV
                     LTGAMVPYAFGSSDGMFNLGSALSFVQVLPPGVYIAMNGQYFRWDQVRKNKQTGVFER
                     VQ"
     gene            complement(2812..3297)
                     /locus_tag="PKK95_07785"
     CDS             complement(2812..3297)
                     /locus_tag="PKK95_07785"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOC18151.1"
                     /translation="MGDKTGPSLQERYAPRSICFGCGPANEKGLRIRSFPDGDGLVTV
                     WTPEPHHQAFEGAVNGGIIASLFDCHCNWTAAWRLMHDRGSDELPSTVTAEFHVRFRR
                     PTPSGQPLTLVSRVVEASGDRATVEATLEAGGTVCATGGGLFAAVGPGHPAYHRWGAG
                     E"
     gene            3439..6330
                     /locus_tag="PKK95_07790"
     CDS             3439..6330
                     /locus_tag="PKK95_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOC18152.1"
                     /translation="MGLLDRFRSQPRWKHSNPAVRIAAVEELPLDQQDVLLSVAREDR
                     DTAVRLAALRKIISPVAIAGIARDDADARVREEGASLLFDLATGAVEGSEADCLAALE
                     GLSDPRQVVAVAKTASSEAVARAALDRVQDPAAVGSVARRSVHPPVRLEALARVVDPR
                     EVLAVALRSEFKDVSLAAVERLGSDRGALEDVASHARNKSAAKRARALARAIDESASR
                     AAVPAAPEPAAGQEEEEKARRRAALAACERLEAVAAEAMEDGEAAVGEIERSWRLAGG
                     EEMADLAPRFAAARAAAAEALERSRQERSARAAALQAMAEAVAVRRSLCEQLDAVAGE
                     ETEPRLEEARRAWAALPELGDAGEAERWRERLEEACRAAEARHRAAIEHRERREKAAV
                     ICDQVASLAETAAFPQGRAEWQALRRSWNALTAAGFEDQALFARFAEADERLRSKEAE
                     ERQRRAQEQQENLARYQKLCADLEAVPASPSLTLKAADRAIRQARAALEEEKSLPSRQ
                     DHDEVTRRLKAVIAALVPRAQELRDMDEWQRWANAGVQEELCRQVEALVQEEDLAVAA
                     RQLREAQARWKQVATAPRDRSQALWNRFKAACDAVRARCDVYYATRAEEQAASQARKQ
                     ALCEQAEALSGSSDWVRTAEGIQKLQAEWKTIGPAPRDAEKALWDRFHAACDAFFTRR
                     RDDLRQRKGEWAANLAKKEALCARAEELAETTEWASAIDEIKRLQAEWKTIGPVRKAK
                     AEQLWRRFRAACDRFFDRYQHRDQAALAAAEAELEALAAEFESMPPAEGAAADPPEGL
                     AERVAAARRQWTSKLSALPRDRAARLGDRWSRALERVAEAWPSAFAGTDLDLEANVRA
                     MEELCVQIESLSADAPRPAFAAAIEAGEGSPATLLAQQLREALATNTIAGRPDEAARA
                     KAVSEQVRQAQAAWRRIGPVPEAAGRALAARFQRAVQRVHEQRRSSARP"
     assembly_gap    6370..6469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6539..7273
                     /locus_tag="PKK95_07795"
     CDS             6539..7273
                     /locus_tag="PKK95_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC18153.1"
                     /translation="MLLSFAHPDDESFLAGGTACRYAAEGARVVLSCATRGEAGKAGD
                     PPLCTPAELPVVREHELREAAAILGIAAVHLLGYHDRALDTVPPDRICEQLVALIRRE
                     RPAVVLTFDPNGANLHPDHVAISRFTSDAVAAAADPRWFPAQGAAHQVARLVWVAGRH
                     PWDLARDPDAAAKPGVDFAIDVGPWRARRLAALEAHRTQRPSVQRHFLRHADRDLLLS
                     REFFRQGWGPPLTARPAGDLFEGIGD"
     gene            7281..7484
                     /locus_tag="PKK95_07800"
     CDS             7281..7484
                     /locus_tag="PKK95_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18154.1"
                     /translation="MLRVQWVLGVLKVLVLVVLKARRAVAAHRAAEAGAGAEGAAGPA
                     RRSRERNERRRVRVPKCRRASPR"
     gene            7577..9334
                     /locus_tag="PKK95_07805"
     CDS             7577..9334
                     /locus_tag="PKK95_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18155.1"
                     /translation="MLRRADLVRPAEPRTNVLLKLPDGRTFEAPIGMTLAEIFLAIGS
                     LSGRSPATPCAGRLRAVAALVNGRLRDLTTPLTEDASVVPLTPQDADGARIYRRSLSF
                     LLMTAAAEEFPGADVSIEHSATTVGAYFCEVRGHAPFTQDELRRIEARMRAIVKADEP
                     IVRTPMPLTQAIEAVKARGDLETARLLAHRQKDDLVLYSLRGRQDYFQGYMVPSTGCL
                     PHFALHAFPPGFFLQFPHQRQPQALPPITPYPKLFQVYEEAGHWLDRLGIRGAGVLND
                     AIAAGRLQEISLVAEALHEARIASIAQDIASRGERVRVILVAGPSSSGKTTFSKRLAV
                     QLVANGRRPLAVCLDDYFVDREHTPRDAAGNFDYECLEAVDVALFNDHLLRLMAGERV
                     QLPRYDFRSGRRETGLSVALGPDAIVIVEGIHGLNPALVPHLPGHSVYRVYVSALTQL
                     NLDRHNRVSTTDCRLVRRIVRDAAFRGYNATETLRRWEAVTSGEKVHIFPYQENSDSI
                     FNSALVHELAVLRPLAEPLLLQVQHDTPEHLEANRLLSFLQWFLPAPTEVVPDNSILR
                     EFVGGSILESFNPWLERIR"
     gene            9593..>9913
                     /locus_tag="PKK95_07810"
     CDS             9593..>9913
                     /locus_tag="PKK95_07810"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOC18156.1"
                     /translation="MPETPTRQDAITTLADLIKDVPVAMLTTTGMGRLRSRPMVAQRV
                     PFDGALWFLTARSAGKTGEIRDRQAVHVTFVSPDDNRYVWASGTATLVDDPAQVKAIW
                     HDGYR"
BASE COUNT         1394 a         3430 c         3613 g         1376 t
ORIGIN      
        1 acgccgacga tgttcttgcc gatgtcgtgg acgtcgccct tgacggtcgc caggacgatc
       61 cggccctgcg aggggcgcgc ggccgactcg gccgccatca ggggctcgag gaacgacacc
      121 gccttcttca tcgcccgggc gctcttcact acctgcggca ggaacatccg ccccgacccg
      181 aagaggtcgc cgaccacttt catcccgtcc atcagcggcc cctcgatgat gtcgagcggc
      241 cgggggtaga gggcgagggc ctcggcgatg tcggcctcga ggtggtcgag gatgccgtgc
      301 acgagcgcgt gcgagagccg ctgctgcacg gtgcccttcc gccactccgc ctcggcctgc
      361 tcgcgccgcg ccgggccggg gctgccggcg gcctgcacca gccgctcggt ggcgtcggga
      421 cggcggttga agaggaggtc ctcgaccctc tcgagcagct cgggggggat gtcctcatac
      481 accacgagct ggccggcgtt gacgatgccc atgtcgaggc cggcccggat ggcgtggtag
      541 aggaacgcgg agtggatcgc ctcgcgcacc gggtcgttgc cccggaaaga gaacgacagg
      601 ttgctgatgc cgccgctgat cttcgcctcg gggaaccggg ccttcagctc gcgcaccgcc
      661 tcgatgaacg cgactgcgta cgtgtcgtgc tcctcgatcc cggtgccgac tgcgagaacc
      721 gccgggtcga agacgatgtc ggcggggtcg aagccggcct ccgcggtgag caggcgatac
      781 gcgcgggaca gcacctcgac cttccgcgcg gcggtgtcgg cctggccccg ctcgtcgaac
      841 gccatcacga cgaccgcggc gccgtagcgg cgcgcgagcc gggcctgccg gaggaactcg
      901 gcctcgccct ccttgaggct gatcgagttg acgatggcct tcccctggac gcacttcagg
      961 ccggcctcga cgaccggcca gcgcgagctg tcgatcatca cggggagccg ggcgatctcg
     1021 ggctcggtgg cgatgaggtt caggaaccgc gtcatcgccg cctcggagtc gagcatcgcc
     1081 tcgtccatgt tcacgtcgag gatgttcgcc ccgccgcgca cctgctcgag ggcgacgtcg
     1141 gcggccgccg cgaagtcgcc cgaggtgacg aggcggcgga accgcttcga gcccgtgatg
     1201 ttggtccgct cgccgatcat ctggaagttg ctgtcggggc ggatggtgag cgtctcgagg
     1261 ccggcgtact gcgtgaagcg ggggaggtcg ggcaccgccc gcggagcgag cccctcgatg
     1321 gcggttgcga tggccgcgat gtgcgcgggc gtggtgccgc agcagccgcc gaccaggttc
     1381 acgaggcccg cctcggcgaa ctcgcggatc gcggccgccg tctgctcggg cgtctcgtca
     1441 tagccgccga aggcgttggg gagcccggcg ttcgggtagc agctgatgcg cgtggcggcg
     1501 atgcgggaga gttcggcgag gtacgggcgc atctcgcgcg ccccgagcgc gcagttgatg
     1561 ccgaccgtca ccggcctggc gtgcgccacc gagacccaga acgcgtccac cgtctgcccc
     1621 gagagggtgc ggccgcttcg atcggtgatc gtcaccgaga tcatgagcgg caggcgccgc
     1681 ccgcgctctt cgaagagatc ctcggcggcg gcgattgccg cctttgcgtt gagcgtgtcg
     1741 aagatcgtct cgatcagcag cgcgtcgcag ccgccgtcca cgagcccgcg aacctgttcg
     1801 aggtaggccg cgcgcagctg atcgaacgtg atcgcccgga acgccgggtc ggcgacgtcg
     1861 ggagaaagcg agagcgtgcg gttggtgggg ccgacggcac cggccaccag gcgcggccgc
     1921 cccggcgtgc gggccgtcca ggcgtcggcg gcctggcgcg cgagccgggc ggcagcgacg
     1981 ttcatctcgt agacgaaccg ctcgcagccg tagtcggcct gggcgaccga ggtggcggtg
     2041 aaggtgcagg tctcgaccag gtcggccccg gccgcaaggt actcctcgtg gatgccacga
     2101 atgaggtcgg gctgtgtcag gacgaggacg tcggtgttgc cgcggagatc gcgcgggtga
     2161 tcgcggaagc ggtcaccgcg gaagccggcc tcgtcgaggc cgtggcgctg caccatggtg
     2221 cccatggcgc cgtcgagcaa gaggatccgt tcggcgagca ggtcggtcag ggttggggac
     2281 atcgatacgg atcattgtac acttcccccc atgcccatcc gggttctcat cacgggcggc
     2341 accttcgaca aggagtacga cgagatcaac gcccggctcg tgttcaagga gtcgcacctg
     2401 cccgagatgc tccgcctcgg acgctgccgc ctcgacctga acgtgcggtc gatcatgatg
     2461 atcgacagtc tcgacatgac cgaggccgac cgggaggtcg tggtgcagaa ctgccgccag
     2521 tgcgaggaga cccggatcgt cgtcacgcac ggtaccgaca cgatggtcga gacggctgcc
     2581 gagctggcgc gcggcgtccc cggcaagacg atcgtgctga ccggcgcgat ggtcccgtac
     2641 gccttcggca gctcggacgg gatgttcaac ctcggctcgg cgctgtcgtt cgtgcaggtg
     2701 ctgccgcccg gcgtctacat cgctatgaac gggcagtact tccgctggga ccaggtgagg
     2761 aagaacaagc agaccggggt cttcgagcgg gtgcagtaag gggaagagta gtcattctcc
     2821 cgctccccac ctgtggtacg cagggtggcc gggaccgacg gcggcgaaca gcccgccgcc
     2881 cgtcgcgcac accgtgccgc ccgcctcgag cgtagcctcg accgtggcgc ggtcgccgct
     2941 cgcctccacc accctggaga caagcgtgag cggctggccc gacggtgtcg gccggcggaa
     3001 gcggacatgg aattcggcgg tgaccgtcga gggcagctcg tcggagccgc ggtcgtgcat
     3061 caaccgccag gcggcggtcc agttgcagtg gcagtcgaag agactcgcga tgatcccgcc
     3121 gttgaccgcc ccttcgaacg cctgatggtg cggctcgggg gtccagaccg tcaccagccc
     3181 gtccccgtcg gggaagctgc ggatgcgcag ccccttctcg ttggccggcc cgcagccgaa
     3241 gcagatgctg cgcggggcgt accgctcctg gaggcttggc ccggtcttgt cgcccatgcc
     3301 ccagcctcgc acagcgagcg ggaatgtgga aggcaaaagg cacaggcagc gcggatatag
     3361 taaactctct ggctccacac cgccccgcgc ggtcgccacc aacacatctg ccgggcgttc
     3421 ccggagacga aagcaggtat gggtcttctc gacaggttcc gcagccagcc ccgctggaag
     3481 cactccaacc ccgccgtccg aatcgccgcc gtcgaagagc tgccgctcga tcagcaggac
     3541 gtgctgctgt cggtcgcgcg cgaggaccgt gacaccgcgg tgcggctcgc cgcgctgcgc
     3601 aagatcatct ccccggtggc gatcgccggg attgcccgcg acgacgccga cgcccgggtc
     3661 cgcgaggaag gggcgtcgct gctgttcgac ctggcgaccg gcgccgtcga ggggagcgag
     3721 gccgattgcc ttgccgcgct ggaggggctg tccgacccgc gccaggtcgt cgccgtggcg
     3781 aagacggcct cgagcgaggc ggtcgcgcgt gccgccctgg accgggtaca ggacccggcc
     3841 gcggtcggct cggtggcccg ccgcagcgtt catccgccgg tgcgcctcga ggcgttggcg
     3901 cgggttgtcg atccccggga agtgctcgcg gtcgcgctgc gcagcgagtt caaggacgtg
     3961 tcgctggccg cggtcgagcg gctgggcagc gatcgcgggg cgctcgagga cgtggcgtcc
     4021 cacgcacgga acaagtcggc ggccaagcga gcccgcgcgc tggcccgtgc gattgacgaa
     4081 agcgcctcgc gcgcggcggt accagcggcg ccagagcctg cagccggcca ggaagaagaa
     4141 gagaaggcca ggcgccgcgc cgcgcttgcc gcgtgcgagc ggctcgaggc agtggccgcc
     4201 gaggccatgg aggatggaga ggcggcggtc ggcgagatcg agcgaagctg gcggctggcg
     4261 ggtggagagg agatggccga cctggcgccg cgcttcgccg cggcgagggc cgccgcagcg
     4321 gaggcgctcg agcggagccg gcaggaacgc agcgcccggg cggcggcgct gcaggccatg
     4381 gccgaagcgg tggcggtgcg gcgctcgctc tgcgagcagc tcgacgccgt cgcgggcgag
     4441 gagaccgagc cgcggctcga ggaggcgcgc cgcgcgtggg cggcgcttcc cgagttgggc
     4501 gatgcgggcg aggccgagcg ctggcgcgaa cggctcgagg aggcgtgccg ggccgccgag
     4561 gccaggcacc gcgcggccat cgagcaccgg gagcggcgcg agaaggcagc cgtcatctgc
     4621 gaccaggtcg cgagccttgc cgagaccgcc gcgttcccgc agggccgcgc cgagtggcag
     4681 gcgctgcgcc ggtcgtggaa cgcgctgacc gccgcgggct tcgaggacca ggccctcttc
     4741 gcccgcttcg ccgaggccga cgagcggctc cggtcgaagg aggcggagga gcgccagcgc
     4801 cgggcgcagg agcagcagga gaacctggcg cgctaccaga agctctgcgc cgacctcgag
     4861 gcggtaccgg cctcgccctc gctcaccctg aaggcggccg accgcgcgat acggcaggcg
     4921 cgcgcggcgc tcgaggaaga gaagtcgctc ccgtcgcgcc aggaccacga cgaggtgact
     4981 cggcgcctca aggccgtcat cgccgcgctg gtcccgcgcg cgcaggagtt gcgcgacatg
     5041 gacgagtggc agcgctgggc caacgccggg gtccaggagg agctgtgccg ccaggtcgaa
     5101 gcgctcgtgc aggaggaaga cctggccgtc gccgcccggc agctgcgcga ggcgcaggcg
     5161 cggtggaagc aggtggccac ggcgccgcgc gaccggtcgc aggcgctctg gaaccggttc
     5221 aaggcggcgt gcgacgcggt tcgcgcccgc tgcgacgtct actacgccac gcgcgccgag
     5281 gagcaggccg ccagccaggc acgcaagcag gcgctctgcg agcaggccga ggcgctctcg
     5341 ggctcgagcg actgggtccg gacggccgag gggatccaga agctccaggc ggagtggaag
     5401 acaatcggcc cggctccgcg cgacgccgag aaggccctct gggaccgctt ccacgccgcg
     5461 tgcgacgcct tcttcacccg ccgccgcgac gaccttcggc agcgcaaggg ggagtgggcc
     5521 gcgaacctgg cgaagaagga ggcgctctgc gcccgcgccg aggagctggc cgagacgacc
     5581 gagtgggcga gcgcgatcga cgagatcaag cggctgcagg ccgagtggaa gaccatcgga
     5641 ccggttcgca aggcgaaggc ggagcagctc tggcggcgct tccgcgccgc gtgcgatcgc
     5701 ttcttcgacc gctaccagca ccgcgaccag gcggcgctcg cagcggccga ggccgagctc
     5761 gaggcgctgg ccgccgagtt cgagtcgatg ccacccgccg aaggggccgc ggccgaccca
     5821 cccgaggggc tcgccgagcg ggtcgcggcc gcgcgccggc agtggacgtc gaagctgtcg
     5881 gcgcttccgc gcgatcgcgc ggcgcgtctg ggcgaccgct ggtcccgcgc cctcgagcgc
     5941 gtggccgagg cgtggccctc cgccttcgct gggaccgacc tcgacctcga ggcgaacgtc
     6001 cgtgcgatgg aggagctgtg cgtgcagatc gagtcgctgt ctgccgacgc cccgcgcccg
     6061 gcgtttgccg ccgccatcga ggcgggcgag gggtcgcctg ccacgctgct ggcgcagcag
     6121 ctgcgcgagg cgctcgccac caacaccatc gcgggccggc ccgacgaggc ggcgcgcgcc
     6181 aaggcggtgt ctgagcaggt gcgccaggcg caggccgcgt ggcggcgcat cggcccggtg
     6241 cccgaggctg ccggccgcgc gctggcggcc cgcttccagc gcgccgtgca gcgcgtccac
     6301 gagcagcgcc gctcctccgc gcgcccgtag cctgatcgcc gcgggaccgg caccctcgcc
     6361 ggtcccgccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tgtcaggcgt
     6481 gccggcgggg ccgatcgtga ccgcacacct cgtgagatag tgggatgtgc ctcacacctt
     6541 gctcctgagt tttgcgcacc cggacgatga gtcgttcctc gcgggcggca cggcgtgccg
     6601 gtacgccgcc gagggcgctc gcgtggtcct ttcgtgtgcg acgcgcggcg aggcgggcaa
     6661 ggcgggggac ccgccgctct gcacgcccgc ggaactgccc gtggttcgtg agcacgagct
     6721 gagagaggcg gcggccatcc tcggcatcgc cgcggttcac ctgctcgggt accacgaccg
     6781 tgcgctcgac accgtgccgc cggaccggat ttgcgagcag ctggtcgcac tgattcgccg
     6841 cgagcggccc gcggtggtgc tcaccttcga cccgaacggc gccaacctgc accccgatca
     6901 cgtcgccatc agccgtttca cctccgatgc cgtggcggcc gcggccgacc cgcggtggtt
     6961 ccccgcacag ggtgcggctc accaggtcgc caggctcgtg tgggtggccg ggcgccatcc
     7021 ctgggacctg gcccgcgacc ccgatgccgc cgcgaagccc ggcgtggact tcgcgatcga
     7081 cgtcggcccg tggcgggcac gccgcctcgc cgccctcgag gcccatcgca cccagcgccc
     7141 cagcgtgcag cggcacttcc tgcgccacgc cgatcgcgac ctgctgctgt cgcgcgagtt
     7201 ctttcgccag gggtgggggc cgcctctcac ggcgcggccc gccggggacc tgttcgaagg
     7261 gatcggggac tgacgctgtc gtgctgaggg tgcagtgggt gctgggggtg ctgaaggtgc
     7321 tggtgctggt ggtgctgaag gctcgccgcg ccgtagccgc gcaccgcgcg gcggaggcag
     7381 gtgctggtgc tgaaggtgcc gccggccctg cccgccgtag ccgcgagcgc aacgagcgaa
     7441 ggcgagtgcg ggtgccgaag tgccggcggg ccagcccgcg gtagccgcga gcgcagcgag
     7501 cgtttggcga aggcgggtgc tgctgcctgg agtgccgctc aggggaattc ggcactgacg
     7561 cagtatcatg tgttcgatgc tgcgtcgcgc cgatctcgtc cgccccgccg agccccgcac
     7621 gaacgtcctc ttgaagcttc ccgacggccg caccttcgag gccccgatcg ggatgaccct
     7681 ggccgagatc ttcctggcta tcggctcgct gtcaggcagg agcccggcga ccccgtgcgc
     7741 gggcaggctg cgcgcggtgg cggccctggt caacgggcgg ctgcgcgacc tgaccacgcc
     7801 gctgaccgag gatgcgtctg tggtgccgct cacgccgcag gacgccgacg gggcccgcat
     7861 ctaccgccgg tccctcagct tcctgctgat gacggccgcg gccgaggagt tcccgggcgc
     7921 cgacgtgagc atcgagcact ccgcgacgac cgtgggcgcg tacttctgcg aggtgcgcgg
     7981 ccatgccccg ttcacccagg acgagttgcg gcgcatcgag gcgcgcatgc gcgccatcgt
     8041 caaggccgac gagccgatcg tgcggacgcc gatgcccctg acgcaggcaa tcgaggcggt
     8101 caaggcccgc ggggacctgg agacagcgcg actgctcgcg caccggcaga aagacgacct
     8161 ggtcctctac agcctccggg gacggcagga ctacttccag ggctacatgg tgccttcgac
     8221 cggctgcctg ccgcatttcg ccctgcacgc gttcccgcct gggttcttcc tccagttccc
     8281 ccaccagcgc cagccgcagg cgctgccgcc catcacgccg tatccgaagc tgttccaggt
     8341 gtacgaagag gcggggcact ggctcgaccg gctgggcatt cgcggcgccg gcgtgctcaa
     8401 cgacgccatt gccgccggga ggctccagga gatctcgctg gtggccgagg cgctgcacga
     8461 ggcgcgcatc gcgagcatcg cccaggacat cgcctcgcga ggcgagcgag tcagggtgat
     8521 cctggttgcg gggccctcct cctcgggcaa gacgacgttc tcgaagcgcc tggcggtgca
     8581 gctggtggcg aacggccggc gcccgctcgc cgtctgcctc gacgactact tcgtggatcg
     8641 cgagcacacg ccgcgtgacg ccgccgggaa cttcgactac gagtgcctcg aggcggtgga
     8701 cgtggcgctc ttcaacgacc acctgctgcg cctgatggcg ggcgagcgcg tgcagctgcc
     8761 gcgttacgat ttccggtccg gccgccgcga gaccggcctc tcggtcgcgc tgggcccgga
     8821 cgccatcgtg atcgtcgagg gcatccacgg cctgaacccg gcgctggtgc cgcacctccc
     8881 gggccactcg gtctaccgtg tctacgtctc ggcgctgacg cagctgaacc tcgaccgcca
     8941 caaccgcgtc agcaccaccg actgccggct cgtgcggcga atcgtgcgcg atgccgcctt
     9001 ccggggatac aacgcgaccg agacgctcag gcgctgggag gcggtcacct ccggcgagaa
     9061 ggtgcacatc ttcccctacc aggagaacag cgactcgatc ttcaactcgg cgctggtgca
     9121 cgagctggcc gtcctgcggc ccctggccga gccgttgctg ctccaggtgc agcacgacac
     9181 cccggaacac ctcgaagcga accgcctgct ctcgttcctc cagtggttcc tgcccgcccc
     9241 gaccgaggtc gttcccgaca actcgatcct gcgcgagttc gtcggtggct cgatcctcga
     9301 gagcttcaac ccctggctgg agcggatccg ttagccctga gctcgccttc gcccgccttc
     9361 gcttcgcctc cggcttgctt gaccttaggt cctcggcgag ctacggcggg gcaggcttcg
     9421 cccccggctt gatgacgtcc tcggcgaact acggcgcgga aagcccctga gccttgagcc
     9481 ctgagcctgc tgttgacgag cacggcactt ctgcggtttg ctagttcaga tagggatcca
     9541 ctcccagatt cactgggcgg cggcccgccg ggggcaccgc cggaggagcg ttatgcccga
     9601 gacgccgacg cgccaggacg ccatcacgac gctggccgat ctcatcaagg acgtgcccgt
     9661 ggcgatgctg acgacgaccg ggatggggcg gctccgcagc cgtccgatgg tcgcgcagcg
     9721 ggtccccttc gacggcgcgt tgtggttcct gaccgcgcgg tcggcgggca agacgggcga
     9781 gatcagggat cgccaggcgg ttcacgtgac cttcgtgtcc ccggacgaca atcgctacgt
     9841 ctgggcttcc ggcaccgcga cgctcgtgga cgacccggcg caggtcaagg cgatctggca
     9901 cgacgggtat cgg
//
LOCUS       DAOPQX010000112         9777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7882_length_9777_cov_3.840979, whole genome shotgun sequence.
ACCESSION   DAOPQX010000112 DAOPQX010000000
VERSION     DAOPQX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_9777_cov_3.840979"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKK95_07815"
     CDS             complement(<1..245)
                     /locus_tag="PKK95_07815"
                     /inference="COORDINATES: protein motif:HMM:NF040902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCxxC motif-containing NuoF prefix
                     domain-containing protein"
                     /protein_id="HOC18157.1"
                     /translation="MNGEVKVREFPRCCSRCWHGPDRPCPQLIDCLSAGPLCHEDEEC
                     AAARSASNARLRREGLARPVIYIGTGTCGLGAGAGKTL"
     gene            complement(242..715)
                     /gene="nuoE"
                     /locus_tag="PKK95_07820"
     CDS             complement(242..715)
                     /gene="nuoE"
                     /locus_tag="PKK95_07820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOC18158.1"
                     /translation="MTVTDIDPIVTKYPAGRRDALIPLLQEVQASQGYLSRDAVVRIG
                     RHLRLPASKVYGVATFYNQFRFQPLGRYHIQVCRGTACHVKGSLAALSTIQRTLSIKP
                     GQTTRDGNFSLEVVACLGACGLAPVMAVNGEFHAGATAERVTKILRTLKREATRK"
     gene            complement(712..1371)
                     /locus_tag="PKK95_07825"
     CDS             complement(712..1371)
                     /locus_tag="PKK95_07825"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOC18159.1"
                     /translation="MPSKPTAAAIPGNTIGRLSLYRRLLQEQLAQGNRQVYSHQLARL
                     AVSTPSQVRRDLMTIGFSGSPRRGYAIGELVSNIEGLLARSIETSVALVGVGNLGRAI
                     LAYYANRQPVRFAAAFDRAPEKTGRILHGCRCYPLSEMAEVIAREQIHVGVIAVPAAE
                     AQGVADQLVLAGVRGILNFAPVRLHVPAGLYVEHVDLMMALDKVAYYAHQQVEQQDSS
                     R"
     gene            complement(1525..2922)
                     /gene="hydG"
                     /locus_tag="PKK95_07830"
     CDS             complement(1525..2922)
                     /gene="hydG"
                     /locus_tag="PKK95_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOC18160.1"
                     /translation="MTTVQAPQFIDEGAIVDTLQAHRQTDSGAVRAVLAKARELHGLE
                     AADVAVLMNVSDPELIGELFQAAAHVKDTIYGRRLVLFAPLYVSNLCGNECTYCAFRA
                     RNTELKRRALSQEEIANEVRILIDQGHKRILLVAGESYPRQGFSYVLDSIDTIYSVKH
                     GKGEVRRVNANVAPLTLDEFRALHERKIGTYQLFQETYHRETYARFHVGGKKKDYDWR
                     VTAMDRAMEAGINDVGIGVLFGLFDWRFELLALMQHIRHLEARFGVGPHTISVPRMEP
                     ATGSDVATHPPHPVSDVDFRKIVAILRLAVPYTGIIMSTRESADIRRETFALGVSQIS
                     AGSRTNPGGYADDERLESSQFCLGDHRGLDEVVRDVAGLGYIPSFCTGCYRLGRTGAD
                     FMDLAKPGDIKQHCDPNGLSTFLEYLIDYASPETRAVGERCIAEALAGMEAWPRKRSE
                     QLMARVQGGERDVYC"
     gene            3134..5470
                     /gene="hypF"
                     /locus_tag="PKK95_07835"
     CDS             3134..5470
                     /gene="hypF"
                     /locus_tag="PKK95_07835"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOC18161.1"
                     /translation="MTDHRGSVERLRVSIRGAVQGVGFRPFVYRLAAEMSLPGWVINS
                     AAGVIVEVEGRPETLREFLVRLERERPPRAVVHGLEPCWLDPVGFERFEIRHSEGGER
                     RTLILPDIAPCDDCLRELRDPADRRYRYPFVNCTNCGPRFTIIEGLPYDRPLTTMKRF
                     RMCPACQAEYDDPRDRRFHAQPNACPACGPRLQLWAPDGRCLYEADEALRYAANAISQ
                     GLIVAVKGLGGFHLVVDARHDAAIERLREAKGREEKPFAVMVAGVDAARHECEVGALE
                     ARLLASPEAPIVLMDRRRGGSIAAAVAPRNPTLGLMLPSTPLHHLLLAELQHPVVATS
                     GNRADETICTDEREALARLAGLADLFLVHDRPIARHADDSIVRVVLGRELLLRRARGY
                     APLPIGLAGTTAARGDEAPSVLGVGAHLKNTVAISVGEAVFVSQHIGDLETPQANEAF
                     EHVIADLERMYQVSRSRVACDLHPDYLSTAYARAAATPAIGVQHHVAHVLSCMAENEQ
                     EPPLLGVAWDGNGFGEDGRIWGGEWFEVAASGVRRVASLSPFGLPGGDAAVRDARRSA
                     AALLFALHGGAALAMDDLAPIASLPPIERRTLRTMLERGLNTPLTSSAGRLFDGVASL
                     LNLRHRVAYEGQAAIELEWAARGGRPGCTYPFVIHEGREFPPGERDEAPALVDWQPML
                     EALIADLRAGAPAADLARRFHDTLAEMIVAVASRQGLERVVLSGGCFQNRCLTERAVT
                     RLREAGFRPFWHQRIPPNDGGIALGQVVAAAWGMGTQR"
     gene            5501..5758
                     /locus_tag="PKK95_07840"
     CDS             5501..5758
                     /locus_tag="PKK95_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOC18162.1"
                     /translation="MCLAIPGKIVSVAGDDPFTRTARVSFDGVIKEVSLAYLPDARVD
                     EYVVVHAGFALSRVDEAEAREVFEYLAAIGVEGGEGPEEPR"
     gene            5755..6858
                     /gene="hypD"
                     /locus_tag="PKK95_07845"
     CDS             5755..6858
                     /gene="hypD"
                     /locus_tag="PKK95_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOC18163.1"
                     /translation="MKYLDEYRDPAAARRTLAAIRALVTRPWTVMEVCGGQTHAILKY
                     GLDQLLPSEVTLLHGPGCPVCVTPAAKIDRAIALASRPEVILCSFGDMLRVPGSAIDL
                     LSAKAAGGDVRVVYSPLDALDLARRERGRQVVFFAVGFETTAPGNAMAAYLARRDGVA
                     NFSLLVSHVLVPPALEAILSSDGCRVRGFLAAGHVCAVTGAAGYLPVARRYRVPIVIT
                     GFEPLDLLMGLHMCLSQLEQGRAEVENQYARVVREEGNPAAQALLHEVFEVVDREWRG
                     IGVIPSSGLGLRRPYEELDAERRFGLEELASPGEPASCQSGLVLQGRLKPSECGAFGK
                     ECTPDHPLGATMVSSEGACAAYYLHRPPSPDAR"
     gene            6865..7917
                     /gene="hypE"
                     /locus_tag="PKK95_07850"
     CDS             6865..7917
                     /gene="hypE"
                     /locus_tag="PKK95_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOC18164.1"
                     /translation="MSDFSASCPAPIARHERVTLAHGGGGRLMHELLSGVFLAAFESP
                     LADRDHDAAVFALGRGPGRVAFTTDSYVVRPLFFPGGDIGTLAVNGTVNDLAMAGARP
                     RFLSAGFIVEEGLEIATLERIARSMQEAAAAAGVAIVTGDTKVVERGRGDGVYVNTAG
                     VGTVEHNLRIAPASVRPGDAVVVSGDLGRHGMAVMAAREGLGFESAIHSDCAPLAEPV
                     LALLEAGLEVHCLRDLTRGGLASALNEIAKASGSGIAIQQQALKVDGGVRAACEMLGL
                     DPFYVANEGRFALFLPESQAGRAVEVLSRFQVTAGAAVAGRVVPTDPGRVVVFGLLGV
                     ARLLPMQSGEQLPRIC"
     gene            7930..8142
                     /locus_tag="PKK95_07855"
     CDS             7930..8142
                     /locus_tag="PKK95_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18165.1"
                     /translation="MIWTFTRDNRQARCEIRRNADGLDYEFVTTSDDGTERVERFDDP
                     AALIDRSVQYFKELFEGGWRPLPDPR"
     gene            8401..8616
                     /locus_tag="PKK95_07860"
     CDS             8401..8616
                     /locus_tag="PKK95_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18166.1"
                     /translation="MSYVRSAQEETLRRVRAEFLEMPGLKLTVPQAQRLWGVDRRTCE
                     AVIEELTASRFLARTRDGAVILRAATA"
     gene            8772..9431
                     /locus_tag="PKK95_07865"
     CDS             8772..9431
                     /locus_tag="PKK95_07865"
                     /inference="COORDINATES: protein motif:HMM:NF017758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOC18167.1"
                     /translation="MAKIDTLEKVMVEELRDILDAEKQVLRSLPKMARAATGGELRMM
                     FEEHRQQTTGQLERLNRAFGLIGSAARGKKCKGMQALIEEAQEMMAEVEAGPVRDAVL
                     IASAQKVEHYEMAAYGTLRTWAARLGYGEVAELLEQTLEQEKLTDGRLTGVAETLANP
                     EAAGEVELEEPRRRAGRSSRAGGAARARKAAGRKASGRKTASRKKTSAGKRAAGRRSN
                     R"
     gene            complement(9524..>9777)
                     /locus_tag="PKK95_07870"
     CDS             complement(9524..>9777)
                     /locus_tag="PKK95_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18168.1"
                     /translation="PASPSAALILVPRLVARLGEGWREWAAAWGPTFVALPPAVTGRR
                     WTNVVTGEQVETFENEGGEAVRVGAALANCPVAILLGQA"
BASE COUNT         1379 a         3256 c         3589 g         1553 t
ORIGIN      
        1 agcgtcttgc cggcgcccgc gccgaggccg cacgtgcccg tgccgatgta gatcaccggg
       61 cgcgccagcc cctcgcggcg cagccgcgcg ttggaggccg aacgggccgc ggcgcactcc
      121 tcgtcctcgt ggcagagcgg gccggccgag aggcagtcga tcagctgggg gcagggccgg
      181 tcgggcccgt gccagcagcg ggaacagcag cgggggaact ccctcacctt cacctcgccg
      241 ttcatttgcg cgtcgcctcc ctcttcagcg tccgcaggat cttggtgacg cgctcggcgg
      301 tcgcgcccgc gtggaactcg ccgttgacgg ccatcaccgg cgccaggccg caggcgccga
      361 ggcaggccac cacttcgagg ctgaagttcc cgtcgcgcgt ggtctggccg ggcttgatgc
      421 tcagcgtccg ctggatcgtg gagagcgcgg cgagcgaccc cttcacgtgg caggccgtgc
      481 cgcggcacac ctggatgtgg tagcggccga gcggctggaa gcggaactgg ttgtagaagg
      541 tggccacgcc gtacaccttg ctcgccggga ggcgcaggtg ccggccgatg cggaccacgg
      601 cgtcgcgcga gaggtagccc tgcgacgcct ggacctcctg gagcagcggg atcagcgcgt
      661 cgcgccggcc cgccgggtac ttcgtgacga tcggatcgat gtcggtgacg gtcatcggga
      721 tgagtcctgc tgttcgacct gctgatgcgc gtagtaggcg accttgtcga gtgccatcat
      781 caggtccacg tgctcgacgt acaggcccgc ggggacgtgc aggcggaccg gcgcgaagtt
      841 gaggatgccg cggacgccgg ccaagacgag ctggtcggcg acgccctggg cctcggcggc
      901 ggggacggca atcacgccca cgtgaatctg ctcgcgggcg atgacctcgg ccatctccga
      961 caaggggtag cagcggcacc cgtggagaat ccggcccgtc ttctcgggcg cccggtcgaa
     1021 ggcggcggca aagcgcaccg gctggcggtt ggcgtagtag gccaggatcg cgcggccgag
     1081 gttgcccacg ccgaccaggg ccaccgaggt ctcgatcgac cgggcaagca gcccctcgat
     1141 gttggagacc agctcgccaa tcgcgtagcc gcgccggggg ctgcccgaga agccgatggt
     1201 catcaggtcg cgccggacct gagagggcgt gctgaccgcg aggcgggcga gctgatgcga
     1261 atagacctgc cggttgccct gggcgagctg ttcctggagc agccggcggt acaagctcag
     1321 ccggccgatg gtattgcccg ggatggcggc ggcggttggc ttgcttggca tagctgtgaa
     1381 tgagttcacg ctgtgaactg tttcacaaat gaagatacac gagctgtcga gccatgtcaa
     1441 gcgcagttgt ctggatatgt gcggcttcgg ccgaatgaat tctggggagg gtagggccag
     1501 cccctagccc ctagccccta gcccctaaca gtacacgtcc cgctcgccgc cctggacgcg
     1561 cgccatcagc tgttcggagc gcttgcgcgg ccaggcctcc atgccggcca gcgcctccgc
     1621 gatgcagcgc tcaccaaccg ccctcgtctc gggtgaggcg tagtcgatga ggtactcgag
     1681 gaaggtcgag aggccgttgg ggtcgcagtg ctgcttgatg tcgcccggct tggcgaggtc
     1741 catgaagtcg gcgcccgtcc gccccaggcg gtagcagccg gtgcagaacg aggggatgta
     1801 gcccagcccg gccacgtcgc gcaccacctc gtcgaggccg cggtggtcgc ccaggcagaa
     1861 ctggctcgat tcgagccgct cgtcgtcggc gtagcccccg gggttggtgc ggctgccggc
     1921 cgagatctgc gacacgccga gcgcgaaggt ctcgcggcgg atgtcggccg actcgcgcgt
     1981 ggacatgatg atcccggtgt aggggactgc caggcgcagg attgcgacaa tcttgcggaa
     2041 gtcgacgtcg ctcacggggt ggggcgggtg cgtcgcgacg tccgagccgg tggccggctc
     2101 catgcgcggc acgctgatgg tgtgcggccc gaccccgaag cgtgcctcca ggtgccggat
     2161 gtgctgcatc agcgcgagca gctcgaagcg ccagtcgaac agcccgaaca gcacgccgat
     2221 tcccacgtcg ttgatgcccg cttccatcgc gcggtccatc gccgtgacgc gccagtcgta
     2281 gtccttcttc ttgccgccga cgtggaaccg cgcgtacgtc tcccggtggt aggtctcctg
     2341 gaagagctgg taggtgccga tcttgcgctc gtggagcgcg cggaactcgt cgagggtcag
     2401 gggcgccacg ttggcgttga cgcgacggac ctcgcccttt ccgtgcttca ccgagtagat
     2461 ggtgtcgatc gagtcgagca cgtacgagaa gccctgccgc gggtacgact cgcccgccac
     2521 cagcaggatc cgcttgtgcc cctggtcgat caggatccgc acctcgttgg cgatctcctc
     2581 ctgcgacagt gcccgacgct tcagctcggt gttccgcgcc cggaacgcgc aatacgtgca
     2641 ctcgttgccg cacaggttgg agacgtacag cggggcgaac agcacgaggc ggcgcccgta
     2701 gatggtgtcc ttgacatggg ctgccgcttg gaacagctcg ccgatgagct cggggtcgct
     2761 gacgttcatg agcaccgcca cgtccgcggc ctccaggccg tggagctcgc gcgccttggc
     2821 aaggaccgcg cggacggcgc ccgaatcggt ctgtcgatgc gcctgcaggg tgtcgacgat
     2881 cgcaccttcg tcgatgaact gcggcgcttg gactgtagtc atgaatccct cgggaatgaa
     2941 cgggaggcgg gaaatccagc cctcgttgct ccgtaaatta tactcctcac tctccacgcc
     3001 ccggggcccg ccttggcccc ctttggtgtc cttcgtgtcc tgtggttcaa accgtcttcg
     3061 tggtcttctc acgggatccg tgagaagatc gggaggacct tccgctggcc gcccgggcgg
     3121 ccgccacctg tctgtgaccg atcatcgtgg ttccgtcgag cggctcaggg tgtcgatccg
     3181 cggcgccgtc cagggcgtcg gcttccggcc cttcgtctac cggcttgccg ccgaaatgtc
     3241 gctgccgggc tgggtgatca actccgccgc cggcgtcatc gtcgaggtgg aggggcggcc
     3301 cgagacgctg cgcgagttcc tggtccgcct cgagcgtgag cgtccgcccc gcgcggtggt
     3361 gcacgggctc gagccctgct ggctcgaccc ggtggggttc gagcgtttcg agatccgcca
     3421 cagcgagggg ggagagcggc gcacgctgat cctccccgac atcgctccct gtgacgactg
     3481 tcttcgcgag ctgcgcgacc cggccgaccg gcgctatcgt tacccgttcg tcaactgcac
     3541 gaactgcggg ccccgcttca cgatcatcga ggggctgccc tacgaccgcc cgctcacgac
     3601 gatgaagcgg ttccggatgt gcccggcgtg ccaggccgag tacgacgatc cgcgcgaccg
     3661 ccgcttccac gcccagccga acgcctgccc ggcctgcggc ccgcggcttc agctctgggc
     3721 gcccgacggg cggtgcctgt acgaggcgga cgaggcgctg cgctacgccg caaacgcgat
     3781 ttcgcagggc ctgatcgtgg cggtgaaggg gcttggcggg ttccacctcg tggtggacgc
     3841 gcggcacgac gcggcaatcg agcggctgcg cgaggcgaag ggacgcgagg agaagccgtt
     3901 tgccgtcatg gtggccggcg tcgacgcggc gcggcacgag tgcgaggtcg gagcgctcga
     3961 ggcgcggctg ctcgcctcgc ccgaagcgcc gatcgtgctc atggaccgga ggcggggcgg
     4021 ctcgatcgca gcggccgtgg cgccgcgcaa cccgacgctc gggttgatgc tgccatcgac
     4081 gccgctgcac cacctgctgc tggccgagct tcagcatccg gtggttgcca ccagcggcaa
     4141 ccgggccgac gagacgatct gcaccgacga gcgcgaggcg ctcgcgcggc tggcgggtct
     4201 cgccgacctc ttcctggtgc acgaccgccc catcgcgcgg cacgccgacg actcgatcgt
     4261 gcgcgtggtg ctcggccgcg agctgctgct tcggcgggcc cgcggctacg ccccgctgcc
     4321 gatcgggctg gccggcacca cggccgcccg cggtgacgag gcccccagcg tgctgggtgt
     4381 cggggcgcac ctgaagaaca cggtggcgat ctcggttggc gaggcggtct tcgtcagcca
     4441 gcacatcggc gacctggaga cgccgcaggc gaacgaggcc ttcgagcacg tgattgcgga
     4501 cctcgagcgg atgtaccagg tttcacgctc ccgggtcgcc tgcgatctcc atcccgacta
     4561 cctctcgacg gcctacgcgc gggcggccgc gactccggca atcggcgtcc agcaccacgt
     4621 ggcgcacgtg ctctcctgca tggccgagaa cgagcaggag ccgccgcttt tgggcgtggc
     4681 gtgggatggg aacggcttcg gcgaggacgg gcgcatctgg ggcggcgagt ggttcgaggt
     4741 tgccgcgtcg ggcgttcgcc gggtggcctc gctgagcccg ttcggtcttc cgggaggcga
     4801 cgcggccgtg cgcgacgcgc gccgctcggc agcggcgctg ctcttcgcgc tgcacggcgg
     4861 ggccgcattg gccatggacg acctggcccc gatcgcttcg ctcccgccaa tcgagcgccg
     4921 cacgctgcgg acgatgctcg agcgtgggct gaacacgccg ctcacctcca gtgccggccg
     4981 gctgttcgac ggcgtggcct cgctgctgaa cctgcggcac cgggttgctt acgagggcca
     5041 ggcagcgatc gagctggagt gggccgcgag gggagggcgg ccgggatgca cgtacccctt
     5101 cgtcattcac gagggccgcg agttcccgcc gggtgaacga gacgaggcgc cggccctggt
     5161 ggactggcag ccgatgctcg aggccttgat cgccgacctt cgggcgggtg cgccggcggc
     5221 cgacctcgcg cgccgcttcc acgacacgct ggccgagatg atcgtggcgg tcgcgtcgcg
     5281 ccagggcctc gagcgcgtgg tgctgtcggg cggctgcttc cagaaccgct gcctgaccga
     5341 gcgggcggtc acccgcctgc gcgaggcggg gttccggcct ttctggcacc agcgcatccc
     5401 gcccaacgac ggcggcatcg cgctggggca ggtcgtggcg gcggcctggg ggatgggtac
     5461 gcaacggtaa acggaggcct gttggcctga ggggagtcag atgtgtctcg ccatcccggg
     5521 aaagatcgtc agcgtggcgg gtgacgaccc gttcacgcgc accgcgcgcg tcagcttcga
     5581 cggcgtgatc aaggaggtga gcctcgccta ccttcccgac gcccgcgtgg acgagtacgt
     5641 cgtcgtccac gccggtttcg ccctcagccg ggtcgacgag gccgaggccc gggaggtgtt
     5701 cgagtacctg gcggccattg gcgtcgaagg tggcgagggg ccggaggagc cgcgttgaaa
     5761 tacctggacg agtaccgcga cccggcggcc gcccgccgca ccctggcggc gatccgcgcg
     5821 ctcgtcacgc ggccctggac cgtgatggag gtctgcggcg gacagacgca cgcgatcctg
     5881 aagtacgggc tcgaccagct gctgccttcc gaggtgacgc tgctgcacgg gcccggctgc
     5941 ccggtgtgcg tgacgccggc ggcgaagatc gaccgggcga tcgcgcttgc gtcgcgtccc
     6001 gaggtcatcc tctgttcgtt cggcgacatg ctgcgcgtcc ccggctcggc aatcgatctc
     6061 ctgtcggcca aggcggccgg cggcgacgtg cgggtggtct actcgccgct cgacgcgctc
     6121 gacctggcgc ggcgcgagcg cgggcgccag gtcgtgttct ttgccgtggg cttcgagacg
     6181 acggcgccgg gaaacgcgat ggcggcgtac ctggcgcggc gggacggcgt cgccaacttc
     6241 agcctgctcg tctcgcacgt gctggtcccg ccggcgctcg aggcgatcct ctcgtcggac
     6301 ggctgccgcg tccggggctt cctggcggcc gggcacgtgt gcgcggtcac cggggctgcc
     6361 ggctacctcc ccgtcgccag gcgctaccgc gttccgatcg tgatcaccgg gttcgagccg
     6421 ctcgacctgc tgatggggct ccacatgtgt ctctcgcagc tcgagcaggg gcgtgcggag
     6481 gtggagaacc agtacgcgcg ggtcgtgcga gaggagggca accccgcggc gcaggcgctg
     6541 cttcacgagg ttttcgaggt ggtcgatcgc gagtggcgag ggattggagt gattccatcg
     6601 agtggcctgg gattgcgccg gccctacgag gagctcgacg cggagcggcg gttcggcctc
     6661 gaggagcttg cctcgccggg ggagccggct tcgtgccaga gcggcctcgt cctgcaggga
     6721 cgcctgaagc cttccgagtg cggcgccttc ggcaaggagt gcacgccgga ccaccccctc
     6781 ggtgcgacga tggtttcttc agaaggagcc tgcgccgcgt actacctcca tcgcccgcct
     6841 tcgccagacg ctcgctgact tcttatgtct gacttctccg cctcctgtcc cgcacccatc
     6901 gcccgccacg aacgggtgac gctcgcccac gggggcggcg gccgcctcat gcacgagctg
     6961 ctgtcggggg tgttcctggc agcgttcgaa agtccgcttg ccgaccgcga ccacgacgcc
     7021 gcggtgttcg cgctgggacg agggccgggc cgcgtggcgt tcacgaccga ttcgtacgtg
     7081 gtgcggccgc tcttcttccc cggaggcgac atcgggacgc tggcggtgaa cggtacggtg
     7141 aacgatctcg cgatggcggg cgcgagaccg cggttcctca gcgccgggtt catcgtcgag
     7201 gaagggctcg aaatcgcaac gctggagcgc atcgcgcgtt cgatgcagga ggctgcggcc
     7261 gcggccggcg tggcgatcgt gacgggcgat acgaaggtgg tggagcgggg ccgcggcgat
     7321 ggtgtgtacg tgaacactgc cggggtgggc accgtcgaac acaacctgcg gatcgccccg
     7381 gcgtcggtca ggcctggcga tgccgttgtc gtcagcggcg acctggggcg ccacgggatg
     7441 gcggtgatgg cggctcgcga gggcctcggg ttcgagtcgg cgatccacag cgactgcgcg
     7501 ccgttggccg agccggtgct ggcgctgctc gaggcgggcc tcgaggtgca ctgccttcgg
     7561 gatctcacgc gcggcgggct ggcgagcgcc ctgaacgaga tcgcgaaggc gtcgggttcg
     7621 ggcatcgcga tccagcagca ggcgttgaaa gtggacggcg gggttcgcgc ggcgtgcgag
     7681 atgctcggcc tcgatccctt ctacgtcgcc aacgaaggac ggtttgcgct cttcctgccg
     7741 gagtcgcagg ccggccgggc cgtggaagtg ttgtcgcggt tccaggtcac cgccggcgcg
     7801 gcggtggccg gacgcgtcgt gcccaccgac ccgggccggg tggtcgtctt cggactgctt
     7861 ggcgtggcgc gcctgctccc gatgcagagc ggcgagcagc tgccgcgaat ctgctaacat
     7921 cagccagcta tgatctggac gttcacacgg gacaatcggc aggcgcgttg cgagatccgg
     7981 cggaacgccg acgggttgga ctacgagttc gtcacgacga gcgacgacgg caccgagcgg
     8041 gtggagcggt tcgacgaccc ggccgccttg atcgatcgat cggttcagta cttcaaggag
     8101 cttttcgaag gcggctggcg gcccctcccc gatccccgct gaggacgccc ctgggccggt
     8161 gtcaccttgg ccgacacaat gtaacggccg cgtcgtcttc ctgacgccgc ggcgtccggg
     8221 ctgcccgatc gcacgccggg agccgcgaaa agctgcgcga ggggaccctc cctccatggc
     8281 accggcattg ctgctcttct tcaggtcagc tgcgcgtcag cactaatcca tcaacccagt
     8341 cgatcgcgaa ccggcggcgg gtcgcggtgt cggggtttga agacaaggag gaggctttct
     8401 atgagttacg tccggtccgc ccaggaagag acgctccgac gcgtgagggc ggagttcctc
     8461 gagatgccgg ggttgaagct gacggtgccg caggcacagc gtttgtgggg cgtcgatcgc
     8521 cgcacctgcg aggccgtgat cgaggagctg acggcgtccc gctttctcgc tcgaacccgt
     8581 gacggcgcgg tgattctgcg cgccgcgacg gcctgaggag cggcgccggc cggaagacca
     8641 cgcgccgcga gcgtgcggcg cgggagcggc gacacgaggt gttcgatggc gcccatcttg
     8701 ctggagttcc tgttgagcgc gtgggtggtc gcagccttgc cacttcccgg cgcgccaagg
     8761 agcagaggcg gatggcgaag atcgacactt tggagaaggt gatggtcgag gagctgcgcg
     8821 acatcctcga cgccgagaag caggtgctca ggtcgttgcc gaagatggcc agggcggcca
     8881 cgggcggcga gctgcgaatg atgttcgagg agcaccgcca gcagacgacg ggccagctcg
     8941 agcggttgaa ccgggctttc gggttgattg gcagcgcggc ccgcgggaag aagtgcaagg
     9001 gcatgcaggc gctgatcgag gaagcccagg agatgatggc cgaggtcgag gcgggcccgg
     9061 tgcgcgacgc ggtgctcatc gcgtcggctc agaaggtcga gcactacgag atggccgcct
     9121 acgggaccct ccgcacctgg gccgcgcggt tggggtacgg agaggtggcc gagctgctcg
     9181 agcagaccct cgagcaggag aagctgaccg acgggcggct caccggcgtg gccgagacgc
     9241 tggccaatcc cgaagcggct ggcgaggttg agctcgagga gccgaggcgc cgcgccggcc
     9301 ggtcgtctcg tgccggcggc gccgctcgtg cccgcaaggc cgccggacga aaggcgtctg
     9361 gccggaagac tgccagccgg aagaagacga gtgccggcaa gcgcgcggcg ggccggcgca
     9421 gcaaccgttg agatggactg acttgccttt gcctccaggc cgctttcttc agcggcctgc
     9481 tggcctcccc caagccgtag cccttgctcc tggcccctgg ccctcaggct tggcccagca
     9541 gtatcgcaac cgggcagttc gcgagcgccg ctccaacccg cacggcttct ccaccctcgt
     9601 tctcgaaggt ttccacctgc tcccctgtga ccacgttggt ccaccgccgt cccgtcacgg
     9661 ccggcggcag cgccacgaag gtcggtcccc acgccgccgc ccactcgcgc cagccttcgc
     9721 cgagcctggc gaccaggcgc gggacgagga tgagggcggc tgagggcgag gccgggg
//
LOCUS       DAOPQX010000113         9769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7887_length_9769_cov_5.212168, whole genome shotgun sequence.
ACCESSION   DAOPQX010000113 DAOPQX010000000
VERSION     DAOPQX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7887_length_9769_cov_5.212168"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKK95_07875"
     CDS             <1..870
                     /locus_tag="PKK95_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOC18169.1"
                     /translation="AFFSAGTLPLRSLAAAEDQGVKFASEMGASSLAELRAKPAAELL
                     AAASKPRQPGFSPILDGYFMPEEPSAIYAAGRHSRVPLLAGWNADEIRQAVTMRPEKP
                     TVASFTADVRKRFGKLADAVLAAYPARDDVEALESAAALASDTFIGYGTWKWIETHLQ
                     SSGSAVYRYSFDRKIPVEPGAAVMGRPVTAADVGARHAGEIEYVFGSLKLSLPKVPWE
                     EADWKLSDAMTSYWANFARSGDPNGSGLPPWPRYEDNGRRVLHLDVTIKDAPDTLRPR
                     YEALDAFAASQRK"
     gene            1107..2798
                     /locus_tag="PKK95_07880"
     CDS             1107..2798
                     /locus_tag="PKK95_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOC18170.1"
                     /translation="MKRNRREFVQVVGAGAAGLTIGGGRWAEAAALPHGAAPAGEDDG
                     PVLLVGEKIAVTETASGKVRGYVLRGIHHFLGIPYGADTSGPNRFMPPRKPKPWADVY
                     PALWWGNSAPQNMENRYASKYASFRDRWNYDDVSEDCLRLNVFTPALDGAKRPVMFWI
                     HGGGFTAGNGIEQDGYNGENLARRGNVVFVSLNHRLGQLGFTNLAGACGEAFAASGNA
                     GMLDIVAALEWVRDNIAAFGGDPGNVTIMGQSGGGAKVCTLTAMPSAKGLFHKAVVLS
                     GASTKSGDKEYSEKLGAYVLKEAGLTPAGIGKLQEMPWKEYYALAGQALRKLSAETGP
                     GGGLRRGFSPSVDGVILPQHPYFPEAAPTAAQVPMLICSTFHEQSASWMDAKLEDMTL
                     EQVVERVKERAGFGPGFGEKAADVVAAYAKAFPGRKPVEIWSLVTSNRQSVVALADAK
                     SRQPAPVYVSWFGWQPPLFDGRLRAFHCVDICFWFYNTDLMISHTGGGARPRKLAAQM
                     SDYLVQFMKTGDPNGGGRPGWPKYTSANGETMILDDVCEVKNDPDREARKALPAV"
     gene            2835..3956
                     /locus_tag="PKK95_07885"
     CDS             2835..3956
                     /locus_tag="PKK95_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOC18171.1"
                     /translation="MPPEDPEAPDLGRASFRALVESAPDAIVAVDARGLIVLANAQAE
                     AVFGYARQELIGQPVELLIPEARRAEHVSARQRYNGQPRRRPMGLGLDLQGRRKDGSV
                     FPTEISLSPLPAPGGPIVIAFIRDITERKKIDDERARWMREEAASRAKDDFVTTLSHE
                     LRTPLNAILGWTEMLKANPGDPAFAAKAIETIQRSARAQVQLVDDLLDVSRIVAGKLR
                     LDLRETELPPVLRAAVETVRPAAEAKTIRLESEISDDLPRVRGDAARLQQVAWNLLAN
                     AVKFTPDGGTVGLAAGRRDADVEVRVSDTGPGIDPAILPHIFERFSQGEQAGPSRQRG
                     LGLGLSIVHDIVQLHGGRVEADSAGPGRGATFIVRLPAL"
     gene            4060..4479
                     /locus_tag="PKK95_07890"
     CDS             4060..4479
                     /locus_tag="PKK95_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HOC18172.1"
                     /translation="MSVRYFAMAAGLVYLLVGVLGFIPGVKTVPHTGDPSLAVHQGYG
                     YLLGLFPVNILHNLVHMAIGFWGMAAYATFEASRTFARGLAIIYGLLAVMGLFPVLNT
                     TFGLVPIFGHDVWLHALTALAAAYFGWLVGVPRVTRA"
     gene            4501..4962
                     /locus_tag="PKK95_07895"
     CDS             4501..4962
                     /locus_tag="PKK95_07895"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC18173.1"
                     /translation="MFTSNAVRSCVELHETEMNRFYEVWLAFRASGAALPTSADSTYA
                     STNHLGGHVLRAARNYLTWIGDTVGRPITDVDLDTDTVSVASKGRAFLDEVLAGWRRH
                     LAAVDARELMEITRKSRWGQDYTVEQMLEHAIVHPMRHRVQLERLMGARAV"
     gene            complement(5014..5181)
                     /locus_tag="PKK95_07900"
     CDS             complement(5014..5181)
                     /locus_tag="PKK95_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18174.1"
                     /translation="MRLPIQFRLATRDEFALLASRAGFDIVWITAGFSAEPYREGECR
                     TAVWTLAPSGA"
     gene            complement(5241..6263)
                     /locus_tag="PKK95_07905"
     CDS             complement(5241..6263)
                     /locus_tag="PKK95_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC18175.1"
                     /translation="MSADDRAPGQQGTGAVRRFTHPGFLDLQVNGFAGVDFNDPATTP
                     DQVRHAAAVMRQHGVTRFLPTIISSSAATFETCARTVLRANDAAIAGIHLEGPYISPE
                     DGARGAHRREDVAPASIDDFKRRQDAAGGRIRLVTVAPEVPGAIALIEHLRASGIRVA
                     IGHTAASPEQIREAVRAGATLSTHLGNGCANMLPRHPNFIWEQLAADELAASLIVDGH
                     HLPPATVKTMVRAKTPRRVVLVTDAIAAAGQPPGEYQLGALRVRLDETGRAAVPGQPN
                     LAGSALSLDRAVGNVVKFAGISLEEALAMASTQPAAYLGVEPGGSLDLEWDPARFTLR
                     VLKANG"
     gene            complement(6510..7751)
                     /locus_tag="PKK95_07910"
     CDS             complement(6510..7751)
                     /locus_tag="PKK95_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC18176.1"
                     /translation="MFFLSTVINYIDRQTLSVLAPYLKTEFTWSNSDFALIVISFRVA
                     YAFGQTGSGQVLDRIGTRKGLSFGVAFYSLAAMLTSLASGLRSFCFFRFLLGAGESAN
                     WPGATKGVSEWFPRRESGWAVALFDSGSSIGGALAPLIVLWIYHTFGSWRPAFIITGS
                     LGFCWLLLFRWFYHSPEEHPRLSAEERDLILSDRADRQADPGAAATRLPAGTLLRLPQ
                     TWGVILAKTLTDPVWFFITDWFAIYLVSRGFRLEDSLVAFWVPFLAADAGNFLGGGVS
                     SALIKRGWSVGRARKTVIVLCALGMMFIAAAVVVSSLWWLTAFFAISTLCYAALSTMI
                     LNLPADIYPTHSVASVSGMSGTGAGIGTIAATYLTGVVADHYSFAPILVTASLVPLVA
                     ALIVLVFVRNTKHSGTMLNRI"
     gene            complement(7941..8912)
                     /locus_tag="PKK95_07915"
     CDS             complement(7941..8912)
                     /locus_tag="PKK95_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOC18177.1"
                     /translation="MRAARDKRETMLALAEKGRKTREELARGDYVPAAFFIHFDRAFH
                     YGQPAVQKHLEYFRYTGMDFVKIQYERTFPPIADIRRPEDWARMPSYKLDFYQPVLDA
                     VQGLVREAKRDALVLMTLYSPYMCAGHTTSLELLTSHLQENPEAVRKGLVAITDSLMG
                     FVRECIRLGVDGFYASTQGGEAGRFADPQVFARHVKPFDLVLMEEINQACPFNILHVC
                     DYNGPYSDLSAYVDYPGHVVNCNPQLTTGKLSWAEITRMFGRPCMGGLDRHGIIAAGS
                     TRQVRQAVSSALKEAAFPFVLGADCTLPGDIKWENIRAAVSAAHDGR"
     gene            9047..>9769
                     /locus_tag="PKK95_07920"
     CDS             9047..>9769
                     /locus_tag="PKK95_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOC18178.1"
                     /translation="MARRTGFASLPLHSGRAPAWLFQRMVRLAREVVVFLAAEQGVEE
                     VLRRLADPFWFQAFGCVLGFDWHSSGVTTTVCGAVKEGLKGLDRELGLYAAGGKGGTS
                     RKTPHEVTAACERLGRDPGRLIYASRMAAKVDSAAVQDGHQLYHHAFFFTEGGEWAVV
                     QQGMNDTSGTARRYHWLSPNVTSFVDEPHDAVCCDVRGDGLNLVAHESAGARDGCAQL
                     AARPPEETLALVAKIPSLALPSR"
BASE COUNT         1552 a         3369 c         3438 g         1410 t
ORIGIN      
        1 gccttcttca gcgccggcac cctgccgctg cggtcgctgg ccgccgccga ggaccagggc
       61 gtgaagttcg cctccgagat gggcgccagt tcgctggccg aacttcgggc gaagccggcc
      121 gccgagctgc tggccgccgc ctcgaagccg aggcagcccg gcttctcgcc gatcctcgac
      181 ggatacttca tgcccgagga gccctcggcc atctacgcgg ccggcaggca cagccgcgtg
      241 cccctgctcg ccggctggaa cgccgacgag atccgccagg cggtgaccat gcggcccgag
      301 aagccgaccg tcgcgagctt caccgccgac gtccgcaagc gctttgggaa gctggccgac
      361 gccgtcctcg cggcttaccc ggcgcgtgac gacgtggagg cgctggaatc ggccgcggcg
      421 ctcgccagcg acacgttcat cggctacggc acctggaagt ggatcgagac gcaccttcaa
      481 tcgagcggct ccgccgtcta ccgctactcg ttcgaccgga agatcccggt cgagccgggt
      541 gcggcggtga tgggccgccc cgtgaccgcg gccgacgtgg gagcccggca cgcgggggag
      601 atcgaatacg tcttcggctc gctgaagctc tcgctgccaa aggtcccctg ggaggaggcc
      661 gactggaagc tgtcggacgc catgacctcc tactgggcga acttcgcgcg cagcggcgac
      721 cccaacggct cgggcctgcc gccgtggccg cgctacgagg acaacggccg ccgggtgctg
      781 cacctcgacg tgacgatcaa ggatgccccc gacacgctgc gcccgcgcta cgaggcgctc
      841 gacgccttcg cggcctcgca acggaagtag gagaggcctt ttctgtccac gttcaccgca
      901 gaggcgcgga gacgcggaga cgacgcggag acgaaggttg accacagagg cacagagagc
      961 acagagacga cacagggaag acggcgagat tgaaaagtaa cctctgcgcc tctgcgtctc
     1021 cgcgttgaat cgttcatctc aagtggccgt gtggcacgtg ttcagctgtc tgctatctgc
     1081 tgtccgcttc atgacgagga gagcgcatga aacgcaatcg acgcgagttc gtccaggtgg
     1141 tcggggctgg agcggccggg ctgacgattg gcggcgggcg gtgggcggag gccgcggcgt
     1201 tgccgcatgg cgccgcgccg gccggtgaag acgacggccc cgtcctgctc gtcggcgaga
     1261 agattgccgt caccgagacg gccagcggca aggtgcgcgg ctacgtcctg cgcggcatcc
     1321 accacttcct cggcatcccg tacggcgccg acacctcggg gcccaaccgc ttcatgccgc
     1381 cgcgaaagcc gaagccctgg gccgacgtct accccgcgct ctggtggggc aactcggcgc
     1441 cgcagaacat ggagaaccgc tacgcgagca agtacgcctc gttccgcgac cgctggaact
     1501 acgacgacgt cagcgaggac tgcctgcggc tgaacgtctt cacgcccgcc ctcgacggcg
     1561 cgaagcggcc ggtgatgttc tggatccacg gcggcgggtt cacggccggc aacggcatcg
     1621 agcaggacgg ctacaacggc gagaacctcg cgcgccgcgg caacgtcgtc ttcgtgtcgc
     1681 tcaatcaccg cctcggccag ctcgggttca ccaacctggc cggcgcctgc ggcgaggcgt
     1741 tcgccgcgtc cggcaacgcc ggcatgctcg acatcgtcgc cgcgctcgag tgggtgcgcg
     1801 acaacatcgc ggccttcggc ggcgaccccg gaaacgtgac catcatgggg cagtcgggcg
     1861 gcggcgcgaa ggtgtgcacg ctgaccgcga tgccctcggc caaggggttg ttccacaagg
     1921 ccgtggtgct cagcggcgcg tccaccaagt cgggtgacaa ggagtactcg gaaaagctgg
     1981 gtgcgtacgt gctgaaagag gcgggcctga cgccggccgg gatcggcaag ctgcaggaga
     2041 tgccctggaa ggagtactac gccctggcgg gacaggcgct gcggaagctg agcgccgaga
     2101 ccggcccggg cggcggcctg cgccgcggct tcagcccgtc ggtggacggc gtaatcctgc
     2161 cgcagcaccc gtacttcccc gaggccgcgc ccacggcggc gcaggtgccg atgctcatct
     2221 gctcgacctt ccacgagcag tcggcgagct ggatggacgc gaagctcgag gacatgacgc
     2281 tcgagcaggt ggtcgagcgc gtgaaggagc gcgccggctt cggccccggc ttcggcgaga
     2341 aggcggccga cgtcgtcgcg gcctacgcga aggcgttccc cggcaggaag ccggttgaga
     2401 tctggtcgct cgtcacaagc aaccgccagt cagtggtggc gttggccgac gcgaagtcgc
     2461 ggcagccggc gccggtgtac gtgagctggt tcggctggca gccgccgctc ttcgacggac
     2521 ggctccgggc cttccactgc gtggacatct gcttctggtt ctacaacacc gacttgatga
     2581 tcagccacac gggcggcggg gcgcggccgc gcaagctggc ggcgcagatg tccgactatc
     2641 tcgtgcagtt catgaagacc ggcgatccca acggcggcgg acggccgggc tggccgaagt
     2701 acacctccgc caacggcgag acgatgatcc tcgacgacgt gtgcgaggtg aagaacgatc
     2761 ccgaccgcga ggcacggaag gctttgccgg cggtctgatc ccagctatag ttggatattc
     2821 gccactaagc ccccatgccc cccgaagacc ccgaggcgcc ggacctcggc cgggcgagct
     2881 ttcgcgccct ggtggagtcg gcgcctgacg ccatcgtcgc cgtggacgcc cgcggcctga
     2941 tcgtgctggc caacgcgcag gccgaggcgg tgttcgggta cgcgcggcaa gagctgatcg
     3001 gccagccggt cgagctgctc atacccgagg cgcgccgcgc ggagcacgtc tccgcgcggc
     3061 agcgctacaa cgggcagccg cgtcggcgcc ccatggggct cgggctcgac ctccagggcc
     3121 gccggaagga cggctccgtc ttccccaccg agatcagcct cagcccccta ccggccccgg
     3181 gcggcccgat cgtcatcgcg ttcatccgcg acatcaccga gcgcaagaag atcgacgacg
     3241 agcgcgcgag gtggatgcgc gaggaggcgg caagccgcgc gaaggacgac ttcgtgacca
     3301 cgctgtcgca cgagctgcgg acgccgctga acgcgatcct cgggtggacc gagatgctga
     3361 aggccaaccc gggcgacccg gcgttcgcgg ccaaggcgat cgagacaatc cagcgcagcg
     3421 cccgcgcgca ggtgcagctg gtggacgacc tgctcgacgt gtcacgcatc gtcgccggca
     3481 agcttcgcct cgacctccgc gagacggaac tgccgccggt gcttcgtgcc gccgttgaaa
     3541 cggtcaggcc ggctgccgag gcgaagacga ttaggctgga gagcgagatc tcggacgacc
     3601 tgccgcgcgt ccggggcgac gcggcccggc tgcagcaggt ggcctggaac ctcctggcga
     3661 acgcggtcaa gttcacgccc gacggcggaa cggtcggcct ggccgcggga cgccgcgacg
     3721 ccgacgtcga ggtgcgggtg agcgacaccg ggccgggcat cgacccggcg atcctcccgc
     3781 acatcttcga gcgcttcagc cagggcgagc aggccggccc ctcgcgccag cgcggactcg
     3841 gtctcggcct ctcgatcgtc cacgacatcg tccagctgca cggagggcgc gtcgaggccg
     3901 acagcgccgg cccgggccgc ggcgccacct tcatcgtccg gctgccggcg ctgtaagccg
     3961 tttcctggct ccccgcttgc acccctcgcg ccctgaactc tgtcagggtt tcgaggcgaa
     4021 acacggcaca gccgacgcgc cgttttgagg gagatggcaa tgagcgttcg atactttgcg
     4081 atggcagccg ggctggtcta cttgctggtc ggcgtgctgg ggttcatccc tggcgtgaag
     4141 acggtgcccc acacgggcga cccgtccctg gcggtgcacc agggctacgg ctacctgctg
     4201 gggctgttcc cggtgaacat cctgcacaat ctcgtgcaca tggcgatcgg gttctggggc
     4261 atggcagcct acgcgacctt cgaggcgtcg cgaaccttcg cgcgcgggct ggcgatcatc
     4321 tacggcctgc tcgcggtcat gggcctgttc ccggttctca acaccacgtt cgggctggtg
     4381 ccgatcttcg ggcacgacgt ctggctgcac gcgctgaccg cgctggcggc ggcgtacttc
     4441 ggctggctgg tcggggtccc gcgggtcacg agggcgtaga aagaaggaga gagaggccga
     4501 atgttcacga gcaacgccgt ccggagctgc gtcgagctgc acgagaccga gatgaaccgc
     4561 ttctacgaag tctggctcgc ctttcgcgcc tccggcgcgg cgttgcccac gagcgccgat
     4621 tccacctacg cctcgacgaa ccacctgggc ggccacgtcc tccgcgccgc ccgtaactac
     4681 ctcacctgga tcggtgacac cgtggggcgg ccgatcaccg acgtcgatct cgacaccgac
     4741 acggtcagcg tcgccagcaa ggggcgcgcg ttcctcgacg aggtgctggc cggatggcgc
     4801 cgccacctcg ccgcggtgga cgctcgcgag ctgatggaga tcactcgcaa gtcacggtgg
     4861 ggccaggact acacggtcga gcagatgctc gagcacgcga tcgtccaccc gatgcggcac
     4921 cgggtccagc tggaacggct gatgggcgcc cgggccgtat agagggccgc tcgcgcgtgt
     4981 gcttcacagg gggtgctcgg ggatgccggc gcttcatgcc ccggacgggg cgagggtcca
     5041 caccgcggtc cggcactcgc cctcgcgata cggctcggcc gagaagccgg cggtgatcca
     5101 gacgatgtcg aagccggctc gcgaggcgag cagcgcgaac tcgtcgcgag tggcgaggcg
     5161 gaattggatc gggagccgca ccagcggcgc gtcggacgcc gcgtcccgga cgcactcctg
     5221 tcctccgggg accgctcctc ctacccgttc gccttcagga cccgcagcgt gaagcgcgca
     5281 ggatcccact cgagatcgag cgacccgcca ggctcgacgc caaggtaagc ggctggctgt
     5341 gtcgaagcca tcgccagcgc ctcctcgagc gaaatgccgg cgaacttcac cacgttgccg
     5401 acggccctgt cgagcgacag tgccgacccg gccaggttcg gctggccggg caccgccgcg
     5461 cgccctgttt cgtcgagcct caccctcagc gcacccagct ggtactcgcc cggaggctgc
     5521 ccggctgccg cgatggcgtc agtgaccagc acgacgcgcc gcggggtctt cgcccggacc
     5581 atcgtcttca cggtggcggg cggcaggtga tggccgtcca cgatcagcga cgccgccagc
     5641 tcgtcggccg ccagctgctc ccagatgaag ttcgggtggc gcggcagcat gttcgcgcag
     5701 ccgttgccca ggtgcgtgga gagggtcgct ccggcgcgca ccgcctcgcg gatctgctcg
     5761 ggagatgccg ccgtgtgccc gatggccacg cggatccccg aagcacgcag gtgctcgatg
     5821 agcgcgattg cgccgggcac ctcgggcgcg acggtcacga ggcggatgcg cccgccggcc
     5881 gcgtcctggc gccgcttgaa gtcgtcgatc gaggcaggcg caacgtcttc gcggcggtgg
     5941 gcgcctcgcg cgccgtcctc gggcgagatg tacggtccct cgaggtggat gccggcaatc
     6001 gccgcgtcgt tcgcccgcag gacggtccgc gcgcaggtct cgaaggtggc ggccgacgag
     6061 gagatgatcg tcggcagaaa ccgggtgacg ccgtgctggc gcatcaccgc ggcggcgtgc
     6121 cgcacctggt cgggggtggt cgccgggtcg ttgaagtcga cgcccgcgaa gccgttcacc
     6181 tgcaggtcga gaaagcccgg gtgcgtgaac cggcgcacgg cgcccgttcc ctgctgccct
     6241 ggcgcgcggt cgtcagccga catggccgcg ccagcgccgg ccgccgcggc ggctgcggac
     6301 gccgccgtca gtgagcaggc ggcacctccg gtgacagcaa gaaagcggcg tctcggcacc
     6361 cggcccggtt tcgcgtctct catcccttca cccccgattg atcccgctga tcttaccgcg
     6421 ctccgaaggg tggccagacg cgccgcggct tactgtccgc cagaacgcgt ctggccactg
     6481 tctgctgtca gctgtcagct gtccgctacc tagatccggt tgagcatcgt tcccgagtgc
     6541 ttcgtgttcc tcacgaacac caggacgatg agcgcggcga ccagcgggac gaggctggca
     6601 gtgaccagga tcggcgcgaa ggagtaatgg tcggctacca cgccggtcag gtaggtggcc
     6661 gcgatggtcc cgatgccggc gcctgtgccg ctcatcccgc tcaccgacgc caccgagtgc
     6721 gtcgggtaga tgtcggccgg caggttcagg atcatcgtcg agagcgccgc gtagcagagc
     6781 gtcgagatgg cgaagaacgc ggtgagccac cagaggctcg acaccaccac cgccgccgcg
     6841 atgaacatca tgccgagcgc gcagagcacg atcaccgtct tgcgcgcccg tcccaccgac
     6901 cagccccgct tgatgagcgc gctcgacacc ccgccgccga ggaagttgcc cgcgtcggcc
     6961 gccaggaacg gcacccagaa ggccaccagg ctgtcctcga gccggaagcc gcgcgagacc
     7021 aggtagatcg cgaaccagtc ggtgatgaag aaccacaccg ggtcggtgag cgtcttcgcg
     7081 aggatgacgc cccaggtctg cggcaggcgc agcagcgtgc cggccgggag ccgcgtggcg
     7141 gcggcgcccg ggtcggcctg ccggtcggcg cgatcggaca ggatcaggtc gcgctcctcg
     7201 gccgagaggc gcgggtgctc ttcgggcgag tggtagaacc agcggaagag cagcagccag
     7261 cagaacccga gcgagccggt gatgatgaac gccggccgcc agctgccgaa cgtgtggtag
     7321 atccagagca cgatcagcgg cgccagcgcc ccgccgatcg acgagccgct gtcgaagagc
     7381 gccaccgccc aaccgctctc gcggcgcggg aaccactccg acaccccctt ggtggccccc
     7441 ggccagttcg ccgattcccc cgccccgagc aggaagcgga agaagcagaa cgagcgcagc
     7501 cccgacgcca gcgacgtcag catcgccgcc agcgagtaga aggcgacgcc gaagctcagg
     7561 cccttgcgcg tcccgatgcg gtcgagcacc tgccccgatc cggtctgccc gaaggcgtag
     7621 gccactcgga acgagatcac gatcagcgcg aagtcggagt tgctccaggt gaactccgtc
     7681 ttcaggtagg gcgccagcac gctcagcgtc tggcggtcga tgtagttgat gacggtcgag
     7741 aggaagaaca acccgccgat gtaccagcgg aggtggcgaa ttggccttct ggtggtgacg
     7801 gaaagggaac cggggcgtgg ccggagcgca gacatggtgg ggaccctacc acaacgccca
     7861 tgctgttcgg gacgattttc gccaggggga ggaccgcggc gggcagcatt ccggctgccc
     7921 gccgtgcagg caccaacgac ctagcgcccg tcgtgcgccg ccgagacggc cgcgcggatg
     7981 ttctcccact tgatgtcgcc cggcagcgtg cagtcggcgc cgagaacgaa cggaaaggcg
     8041 gcctccttca acgccgagga cacagcctgc cgcacctgcc gcgtgctgcc ggccgcgatg
     8101 atgccgtggc gatcgaggcc gcccatgcac ggccggccga acatccgcgt gatctccgcc
     8161 cacgacagct tcccggtggt gagctgcggg ttgcagttca ccacgtggcc cgggtagtcc
     8221 acgtacgccg agaggtccga gtacggcccg ttgtagtcgc acacgtgcag gatgttgaac
     8281 gggcaggcct ggttgatctc ctccatcagc acgaggtcga acggcttgac gtgacgggcg
     8341 aacacctgcg ggtcggcgaa ccggcccgcc tcgcccccct gcgtggaggc gtagaagccg
     8401 tccaccccga gccggatgca ctcgcgcacg aaacccatca ggctgtcggt gatggccacc
     8461 agccccttcc gcaccgcctc ggggttctcc tgcaggtgcg aggtcagtag ctcgaggctt
     8521 gtggtatgcc ccgcgcacat gtagggcgag tagagcgtca tcaggacgag cgcgtcgcgc
     8581 ttcgcctcgc ggacgagccc ctgcacggcg tcgagcaccg gctggtagaa gtcgagcttg
     8641 tacgacggca tgcgcgccca gtcttccggc cgacggatgt cggcgatcgg cgggaacgtg
     8701 cgctcgtact ggatcttcac gaaatccatg ccggtgtagc ggaagtactc gaggtgcttc
     8761 tgcacggccg gctgcccgta gtggaacgcc cggtcgaagt ggatgaagaa ggcggccggg
     8821 acgtagtctc cgcgagccag ctcttcgcgg gttttccgcc ccttctctgc aagcgccagc
     8881 atcgtctcgc gcttgtcgcg tgcggcgcgc atgacctttc ctcctcgccc gacggcggcg
     8941 gccgacaggg ccgcctgcag gaaacggcga cgtgatgggt ggctgctcat ctcgtccccc
     9001 tcgaattacc ggcgttcacc gccgcatcat atgccagaat cggcgaatgg cgcggcgcac
     9061 aggattcgca tcgctgccgc ttcactcggg ccgggcgccg gcctggctct tccagcggat
     9121 ggtgcgcctc gcgcgcgagg tcgtcgtctt cctcgcggcc gagcagggtg tcgaggaggt
     9181 gctgcgacgc ctggccgacc ctttctggtt ccaggcattc gggtgcgtgc tgggattcga
     9241 ctggcactcg agcggcgtga ccacgacggt gtgcggcgcc gtgaaggagg ggctgaaggg
     9301 gctcgaccgc gagctggggc tgtacgcggc cgggggcaag ggcgggacct cccgcaagac
     9361 gccgcacgag gtgacggcag cctgcgagcg gctcgggcgc gaccccgggc ggctcatcta
     9421 tgcgagccgc atggcggcaa aggtcgacag cgcggccgtg caggacggcc accagctcta
     9481 ccaccacgcg ttcttcttca cggagggcgg cgagtgggcg gtggtgcagc agggcatgaa
     9541 cgacacaagc ggcacggcgc gccgctacca ctggctgtcg cccaacgtca cgagcttcgt
     9601 ggacgagccg cacgacgcgg tgtgctgcga cgtgcgcggg gacgggctca acctcgtcgc
     9661 ccacgagagt gccggcgcgc gcgacgggtg cgcgcagctc gccgcacgcc cgccggagga
     9721 gacgcttgct ctcgtcgcga agatcccgtc gctcgcgctc ccttcgcgc
//
LOCUS       DAOPQX010000114         9654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_7989_length_9654_cov_5.273779, whole genome shotgun sequence.
ACCESSION   DAOPQX010000114 DAOPQX010000000
VERSION     DAOPQX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7989_length_9654_cov_5.273779"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKK95_07925"
     CDS             <1..412
                     /locus_tag="PKK95_07925"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOC18179.1"
                     /translation="AQFKAFSDLGLLGLDPIQAGGQQVIPRDVFHALLAPQIQREEFK
                     DVCVMRVTGRGRTGGREAEATIELVDRYDEKTGFTAMQRLTGWHASIMLIAVLDRKVR
                     PGVVSVETAVPGKTVVDECRRRGFAIEETISLEP"
     gene            442..1422
                     /locus_tag="PKK95_07930"
     CDS             442..1422
                     /locus_tag="PKK95_07930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC18180.1"
                     /translation="MSLEPSFSLVAAEGAILEEILDLTHPVWGEGLDRASYGRYNAAQ
                     LRTPWGRSHLRRVALVDAEGRWVSTAKRYDLRVRAGGREVPALGIGALFTRPEARRRG
                     AASALLRALLAAGAQEGARVALLFSEIGTGYYERFGFRAIPVTQTVLELTARPAAAPP
                     AIPMRSGEPADLPAIAEMNQGQAGAFAFTLVRSPEYVGFAIAKKRLLAASGVPGHREV
                     EFFVVEEGGRAAAYAVVLAVGDYWMVTECGDRDPSGARVGALIEAMLSRPGTRVARIL
                     AWLPPNFLPPQLSVLWRERPAVAMMAAPLGGFPLVVPHRDEVAWWHADAF"
     gene            1545..4868
                     /locus_tag="PKK95_07935"
     CDS             1545..4868
                     /locus_tag="PKK95_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOC18181.1"
                     /translation="MKRTTCLTACVLAMVLAFATLAAAIDTSDTRLLSQPAISGSNLA
                     FVYAGDLWVCGLDGRNVRRLTSSQGVSNPAFSPDGSLVAFTAQYEGNADVYVVPVAGG
                     VPRRLTWHPGRDGVQGFTPDGQSIVFSSSRTVFSGRYSQLFTVPVKGGAPVRLDPPYA
                     FRGALSPDGTRIAYNPLYDAFTQWKHYRGGTHSVIWIYTTATRGVDAVPQPPDRPNDV
                     YPAWIGDTLYFVSDRAGEFNLFSYDTKSKKVTQLTRHDDFPVLYASAGGGKVVYEQAG
                     YLHVFDPAAGKSAKVTVGVTADLPDVRPRFVKGARHIRNVGLSPSGARAVFETRGEIV
                     TVPAEKGDPRNLTNTTAVHERSPAWSPDGKSIAYFSDAGGAYQLVIEAQDGKGEPRRY
                     KLNGAGFYDRPAWSPDSRKIAYTDNAWTLSVLDVASGATKTIATEPIYGPFKTIGASW
                     SPDSKWIAYTLNTRNWIQQVHLYSVEQGKSFPVTDGLSDVTGPVFDRSGKYLYMLGST
                     DAGPVRDWFAMSNADMRATSAIYLAVLDKEAASPLAKESDEEGKKPEGEEKKDEKPIQ
                     KPGDKEKGAAAAKPEAEKPAADKAPIPPVVKIDVEGLPNRILTIPVPAAELSGLQAGE
                     ANQIYYARSVDRRTELHRYDLGTRKDETLLPDVRGYLVSADGKKLLYRAGENWAIAST
                     KAKIEPSQGRLNLDALEVRVDPAAEWPQIYDEAWRVNRDYFYDPTMHGVDWPAMRKKY
                     SVFLPHVAVRDDLNRVIQWMCSELGVGHHRVGGGENRTEPATVPGGLLGADFTIENGR
                     YRFAKVYGGLNWNPELRSPLTEPGVNVRAGEYLLAVNGQDLRADTSVYAPFENTAGKI
                     VEITVGPNPDGSGSRTVSVVPIANEGALRNRDWVEGNLRRVTEATQGRVAYVYVPNTS
                     TMGHTYFKRYFYPQADRDAIIVDERFNGGGSVADYYTDLLRRPHGAYWATRYGADFAT
                     PTASIQGPKVLLVDETAGSGGDLFPWMWRKFALGPIVGKRTWGGLVGTLGFPVLMDGG
                     MVTAPNLAIWTEDGWVVENEGVPPDIEVEQNPADLIDGKDPQLERAIQIVLEELKKRP
                     PSRPTRPAWRSVPKERGIK"
     gene            complement(4921..5118)
                     /gene="thiS"
                     /locus_tag="PKK95_07940"
     CDS             complement(4921..5118)
                     /gene="thiS"
                     /locus_tag="PKK95_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOC18182.1"
                     /translation="MITVNGEPLDWQPGMTVADVLRIRNYIFRMLVVQVDGELVKRGT
                     YDKAKISDGANVEVIHMISGG"
     gene            complement(5180..7384)
                     /locus_tag="PKK95_07945"
     CDS             complement(5180..7384)
                     /locus_tag="PKK95_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOC18183.1"
                     /translation="MTTTYDYKTRTSYTEDVARQKAAHRVLLRHEFEPSLPVRGYTNR
                     TLHIDLSTLKVSEKKVTQEMKDVFIGGRGFGLWYLWNAIKPTTKWDDPENDIIISPGP
                     LAGNTAYAGSGKSLVVTLSPLTGIPIDSNVGGYFGPLMKFCGYDALEIQGKADRDVVV
                     VIDGPKKTITIEEAPEENYDSHVAAEVFMHEYADDEKDFVNVSVVSAGKGADHSRIGC
                     LNFSWYDPKRGAARLKQAGRGGIGRVFRDKKLKALVVHGFKLRPDMNQAADFKRTTAV
                     GRVLAKEIHDFDDQQCKMRKIGTAHLVEIMDAYDLLPVHNFQYGKHKDIDKIASPVWE
                     KRFTQGIFDGCWYGCHMACAKGADCHKVMTGPYKGHLVTVDGPEYETAAGLGSNCGIF
                     DPDWILESNFYCDTYGVDTISFGTCCAFAMECWQRGILNAERTGGLDLSWGNGASHVE
                     LLHQMARGEGFGVIVGQGVKYMQEYFAEQGWGDLQFMRDFGMQVKGLEYSQYQSKESL
                     AQQGGYALTNKGPQHDEAWLIFMDMVNNQIPTFEDKAEALHYFPLFRTWFGLMGLCKL
                     PWNDIEPEDNAKHGKNAAKVPEHVHNYTELYAGITGQNVTIDDFIRMSEGVYNFQRVF
                     NLRMGVGGRKSDWPPYRSMGPLTVEEYESRAERYDKQLKELIGVDPEGKSTEEKMALH
                     RAWREDRYNKLCDAVYRRRGWTNNAIPTLETLRKFKIDFPEVVEVVRPHLGE"
     gene            complement(7541..9133)
                     /locus_tag="PKK95_07950"
     CDS             complement(7541..9133)
                     /locus_tag="PKK95_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOC18184.1"
                     /translation="MPQSELKPAQAKITYATMSADQMADLHRDLDEAIERVRAGFGRS
                     HPMLVGGRQVEGEGGEFEDRSPIDTRILIGRFTAATREQTREAIAAARRAFPEWSRVG
                     WEERVRIVRRMGQRIRERRAELSAIIGYEVGKNRLECVGEVEEAADFFDYYCDRMEQT
                     DGFANLMGLPGSREESLSVLRPYGVFGVIAPFNFPLALAAGPTAAALLAGNTVVCKPA
                     EDTPLACVPLAEIASEFLPPGAFNLVNGPGVPVGEEIANNDGVDGLVFTGSMEVGMML
                     LRQNGARPVPRPLIIEMGGKNPALVMASADPEKASDGVMRSAFGASGQKCSACSRVYA
                     SRQVRDRFVEMLVEKTRAIKVGNPLERDVWMGPVINERAVRNYEQAIEEAKRDGGRVL
                     VGGRRLSGGLFDHGYYVEPTVIDGLPRDHRLFREELFVPLTVVADVLTLDEALDLANR
                     TSYGLTAGIFSEDEREVETFFEGIQAGVVYANRRAGATTGAWPGQNSFGGWKASGSTG
                     KGTGGPHYLQQFFREQSRTRVR"
     gene            9185..>9654
                     /locus_tag="PKK95_07955"
     CDS             9185..>9654
                     /locus_tag="PKK95_07955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC18185.1"
                     /translation="MASRARLFAISCLAMFVFGMVIALLGTLFGLPETRARLAIDLAQ
                     QGQLLSVMFTGLLAATIVAGPTQDRFGAKLVLVAAAGAVAVALVLLALARSFSSAAVW
                     AGLLGIGAGWLNLGANALVSEVYPESRGRMLNLTNVFFGVGALFVPLLVSVAFGV"
BASE COUNT         1418 a         3403 c         3270 g         1563 t
ORIGIN      
        1 ggcgcagttc aaggcgttct ccgacctggg gctgctcggc ctcgacccga tccaggccgg
       61 cggtcagcag gtgatcccgc gcgacgtctt ccacgcgctg ctcgcgccgc agatccagcg
      121 cgaggagttc aaggacgtgt gcgtgatgcg ggtcaccggg cgcggccgca cgggaggccg
      181 cgaggccgag gccaccatcg agctggtcga tcgctacgac gagaagaccg gcttcaccgc
      241 gatgcagcgg ctgaccgggt ggcacgcctc gatcatgctg atcgcggtgc tcgaccggaa
      301 ggtgcgtccc ggggtggtgt cggtggagac cgccgtccct ggcaagaccg tggtggacga
      361 gtgccggcgc cgcggcttcg cgatcgagga gaccatcagc ctcgagccgt gagcccgcct
      421 tcgccccctg agtcttgaac cttgagcctt gagccttcct tctccctcgt cgccgccgaa
      481 ggggcgatcc tcgaggagat tctcgacctc acgcatccgg tgtggggcga ggggctcgat
      541 cgcgcttcct acgggcgcta caacgcggcc cagctgcgga cgccgtgggg ccgctcgcac
      601 ctgcggcggg tggccctggt cgacgcggag ggccgctggg tctcgacggc aaagcggtac
      661 gacctgcgcg tgagggccgg gggacgcgag gtgccggccc tcggcattgg cgcgctgttc
      721 acccggccgg aggcgcggcg ccggggcgcc gcgtcggcgc tgctgcgggc gctgctggcc
      781 gcgggcgcgc aggagggagc gcgcgtcgcg ctgctcttct cggagattgg caccggctac
      841 tacgagcgct tcggcttccg tgccatcccg gtcacgcaga ccgtcctcga gctgaccgca
      901 cgtccggcgg ccgccccgcc ggcgatcccg atgcgatcgg gcgagccggc cgaccttccc
      961 gccatcgccg agatgaacca ggggcaggcc ggggcgtttg ccttcacgct cgtccgctct
     1021 ccagagtacg ttggcttcgc gatcgcgaag aagcgcctgc tcgcggccag cggagtgccc
     1081 ggccaccgcg aggtcgagtt cttcgtggtc gaggagggtg ggcgcgccgc cgcctacgcg
     1141 gtggtgctcg cggtgggcga ctactggatg gtcaccgagt gcggcgaccg cgacccgtcg
     1201 ggggcgcgcg tcggcgccct catcgaggcg atgctctcgc gccccggcac gcgggtggcg
     1261 cggatcctcg cctggctccc gcccaacttc ctcccgccgc agttgagcgt cctctggcgc
     1321 gagcggccgg ctgtcgcgat gatggcagcc ccgctcggcg gcttccccct cgtcgtgccc
     1381 caccgcgacg aggtcgcctg gtggcacgcc gacgcgttct gagggctttg agcggaccgc
     1441 ggcgcaccgg ctcccgatcc ggccggcggc ggggtcgcgg ggactccgcg gcggcctgga
     1501 gtatgatggc cgccacgtct cgggctttcc cccaggagct gtccatgaag cgcacgacct
     1561 gtctgaccgc ctgcgtcctg gcgatggtgc tggcgttcgc caccctcgcc gccgccatcg
     1621 acacgtccga cacgaggctg ttgagccagc cggccatcag cggatcgaac ctcgcgttcg
     1681 tctacgcggg cgacctgtgg gtgtgcggcc tcgacggccg gaacgtccgc cgcctgacca
     1741 gcagccaggg ggtctcgaac ccggcgttct cccccgacgg atcgctcgtc gccttcaccg
     1801 cccagtacga ggggaacgcc gacgtctacg tggtgccggt ggccggcggc gtgcccaggc
     1861 ggctgacgtg gcacccgggc cgcgacggcg tgcaggggtt cacccccgac gggcagtcaa
     1921 tcgtcttctc gtcgtcgcgc accgtcttca gcggccggta cagccagctc ttcacggttc
     1981 cggtgaaggg gggcgcaccg gtgcggctcg acccgccgta cgccttccgc ggggcgctct
     2041 cgcccgacgg cacgcgcatc gcctacaacc cgctgtacga cgcgttcacc cagtggaagc
     2101 actaccgggg cggtacgcac tcggtcattt ggatttacac caccgcgacg cgcggggtcg
     2161 atgcggtgcc gcagccgccc gaccggccca acgacgtgta tcccgcctgg attggcgaca
     2221 cgctctactt cgtctccgac cgggccggcg agttcaacct gttctcctac gacaccaagt
     2281 cgaagaaggt cacacagctg acccgccacg acgacttccc cgtgctgtac gcgtcggccg
     2341 gcggcggcaa ggtggtgtac gagcaggcgg gctaccttca cgtgttcgat ccggcggccg
     2401 ggaagagcgc gaaggtcacc gtcggcgtga ccgccgattt gcccgacgtc cgtccgcggt
     2461 tcgtgaaggg ggcgcgccac atccggaacg tcgggctttc gccgtcgggg gcgcgggcgg
     2521 tgttcgagac ccgcggcgag atcgtcaccg tgcccgccga gaagggcgac ccgcgcaacc
     2581 tcaccaacac caccgccgtg cacgagcggt cgccggcgtg gtcgcccgac gggaagtcga
     2641 tcgcgtactt ctccgacgcg gggggcgcct accagctggt gatcgaggcg caggacggca
     2701 agggcgagcc gcggaggtac aagctgaacg gcgcgggctt ctacgaccgc cccgcctggt
     2761 cgcctgacag caggaagatc gcctacaccg acaacgcctg gaccctctcg gtactcgacg
     2821 tggcgagcgg cgccacgaag acgatcgcga ccgagccgat ctacgggccg ttcaagacga
     2881 tcggtgcctc ctggtcgccc gactcgaagt ggatcgccta tacgctcaac acccgcaact
     2941 ggatccagca ggtccacctg tactcggtcg agcagggcaa gtcgttcccc gtgaccgatg
     3001 gcctgagcga cgtgaccggg cccgtcttcg accgcagcgg caagtacctg tacatgctcg
     3061 gatcgaccga cgctggcccc gtgcgcgact ggttcgcgat gtcgaatgcc gacatgcgcg
     3121 cgacgagcgc gatctacctg gcggtgctcg acaaggaggc cgcgtcaccc ctggcgaagg
     3181 agagcgacga ggagggcaag aagccggaag gagaggagaa gaaggacgag aagccgatcc
     3241 agaagcccgg cgacaaggag aagggcgcgg ccgcggcgaa gcccgaggcg gagaagccgg
     3301 ccgcggacaa ggcgccgatc ccgccggtgg tgaagatcga cgtggagggg ctccccaacc
     3361 ggatcctcac gatacccgtg cccgccgccg agctttccgg cctgcaggcc ggcgaggcga
     3421 accagatcta ctacgcgcgc agcgtcgatc ggcgcaccga gctgcaccgc tacgacctcg
     3481 gcacgcggaa ggacgagacc ctgcttcccg acgtcagggg ctacctggtg tcggccgacg
     3541 gcaagaagct gctctaccgc gccggtgaga actgggcgat cgcctcgacg aaggcgaaga
     3601 tcgagccgtc gcagggccgc ctcaacctcg acgcgctcga ggtcagggtg gacccggccg
     3661 ccgagtggcc acagatttac gacgaggcgt ggcgcgtgaa ccgcgactac ttctacgacc
     3721 cgacgatgca cggcgtggac tggccggcga tgcggaagaa gtactcggtg ttcctgccgc
     3781 acgtggcggt gcgcgacgac ctgaaccgcg tgatccagtg gatgtgcagc gagctgggcg
     3841 tcggccacca ccgcgtcggc ggcggcgaga accggaccga gccggcgacg gtgccgggcg
     3901 gcctgcttgg cgccgacttc accatcgaga acggccgcta ccgcttcgcg aaggtctacg
     3961 gcggcctgaa ctggaacccc gagctgcggt cgccgctgac cgagccgggc gtgaacgtcc
     4021 gcgccggcga gtacctcctg gcggtgaacg gccaggacct gcgggccgac accagcgtct
     4081 acgccccctt cgagaacacc gccgggaaga tcgtcgagat caccgtgggg cccaaccccg
     4141 acggcagcgg ctcgcggacc gtcagcgtgg tgccgatcgc gaacgagggc gccctgcgga
     4201 accgcgactg ggtggagggc aacctgcggc gagtcaccga ggccacccag ggccgcgtgg
     4261 cctacgtgta cgtccccaac acgtccacga tgggacacac gtacttcaag cgctatttct
     4321 acccgcaggc agaccgcgac gcgatcatcg tggacgagcg gttcaacggc ggcggctcgg
     4381 tggccgacta ctacaccgac ctgctgcgcc ggccgcacgg cgcctactgg gccacccgct
     4441 acggcgccga cttcgccacg ccaaccgcct ccatccaggg gccgaaggtg ctgctggtcg
     4501 acgagacggc cggctcgggg ggcgatttgt tcccctggat gtggcgcaag ttcgccctgg
     4561 ggccgatcgt cggcaagcgg acgtggggcg gcctggttgg gacgctgggc ttccccgtgc
     4621 tgatggacgg cgggatggtg accgcgccga accttgccat ctggaccgag gacggctggg
     4681 tggtggagaa cgagggcgtg ccgcccgaca tcgaggtcga gcagaacccg gccgacctga
     4741 tcgacggcaa ggacccgcag ctcgagcggg cgatccagat agtgctggag gagctgaaga
     4801 agaggccgcc ctcgcgcccc acgcgcccgg cgtggcggag cgtgccgaag gagcggggga
     4861 tcaagtagga gggttcagga atcgggattc aggatccctg aatcctgaat cccgattcgc
     4921 ctaacccccg ctgatcatgt ggatgacctc gacgttcgcg ccgtcggaga tcttggcctt
     4981 gtcgtacgtc ccgcgcttca cgagttcgcc gtccacctgc acgaccagca tccggaagat
     5041 gtagttgcgg atcctgagga catccgccac cgtcatgccg ggctgccagt cgagcggctc
     5101 accgttcacc gtgatcatcg ctaatcgaca acctctctgc gccctccgcg tctctgcggt
     5161 gaatccgtga ccgggatccc tactccccca ggtgcggccg caccacctcc accacctccg
     5221 ggaagtcgat cttgaacttc ctgagcgtct cgagggtcgg gatggcgttg ttggtccagc
     5281 cgcggcgcct gtagaccgcg tcgcagagct tgttgtagcg gtcctcgcgc caggcgcggt
     5341 gaagcgccat cttttcctcg gtgctcttcc cctcggggtc cacgccgatc agctccttca
     5401 gctgcttgtc gtaccgctcg gcgcgcgact cgtactcctc gaccgtcagc ggccccatcg
     5461 agcggtacgg cggccagtcg ctcttccggc cgcccacgcc catccgcagg ttgaacacgc
     5521 gctggaagtt gtagaccccc tccgacatgc ggatgaagtc atcgatcgtg acgttctgcc
     5581 ccgtgatgcc cgcgtacagc tcggtgtagt tgtggacgtg ctcgggcacc ttcgcggcgt
     5641 tcttcccgtg cttcgcgttg tcctcgggct cgatgtcgtt ccacggcagc ttgcagaggc
     5701 ccatcagccc gaaccaggtg cggaacagcg ggaaatagtg aagcgcctcg gccttgtcct
     5761 cgaaggtcgg gatctggttg ttcaccatgt ccatgaagat cagccacgcc tcgtcgtgct
     5821 gcggcccctt gttggtgagc gcgtagccgc cctgctgcgc cagcgactcc ttcgactggt
     5881 actgcgagta ctccagcccc ttcacctgca tcccgaagtc gcgcatgaac tgcaggtcgc
     5941 cccagccctg ctcggcgaag tactcctgca tgtacttcac cccctgcccc acgatcacgc
     6001 cgaacccctc gccgcgggcc atctggtgca gcagctccac gtgcgacgcg ccgttgcccc
     6061 agctcaggtc gaggccgccg gtccgctcgg cgttgaggat gccccgctgc cagcactcca
     6121 tcgcgaacgc gcagcaggtg ccgaacgaga tcgtgtccac cccgtaggtg tcgcagtaga
     6181 aattcgactc gaggatccag tcggggtcga agatgccgca gttcgatccg aggccggcgg
     6241 ccgtctcgta ctcggggccg tccaccgtca ccaggtggcc cttgtacggc ccggtcatca
     6301 ccttgtggca gtcggccccc ttggcgcacg ccatgtggca gccgtaccag cagccgtcga
     6361 agatcccctg cgtgaaccgc ttctcccaca ccggcgaggc gatcttgtcg atgtccttgt
     6421 gcttgccgta ctggaagttg tggaccggca gcaggtcgta cgcgtccatg atctcgacca
     6481 ggtgcgccgt gccaatcttc cgcatcttgc actgctggtc gtcgaagtcg tggatctcct
     6541 tcgccaggac ccggccgacg gcggtcgtgc gcttgaagtc ggccgcctgg ttcatgtcgg
     6601 gcctcagctt gaagccgtgc acgaccagcg ccttgagctt cttgtcgcgg aacacccggc
     6661 cgataccccc gcgccccgcc tgcttcagcc gcgccgcgcc ccgcttcggg tcgtaccacg
     6721 agaagttcag gcagccgatc cggctgtggt cggccccctt gcccgccgac accaccgaca
     6781 cgttcacgaa gtccttctcg tcgtccgcgt actcgtgcat gaagacttcg gccgcgacgt
     6841 ggctgtcgta gttctcctcg ggagcctcct cgatcgtgat cgtcttcttc gggccgtcga
     6901 tgaccaccac cacgtcccgg tcggccttgc cctggatctc gagcgcgtcg tacccgcaga
     6961 acttcatgag cgggccgaaa tagccgccga cgttgctgtc gatcgggatg cccgtcagcg
     7021 gcgacagcgt caccaccagc gacttgcccg atcccgcgta cgcggtgttc ccggccagcg
     7081 ggcccgggct gatgatgatg tcgttctcgg ggtcgtccca cttggtggtg ggcttgatgg
     7141 cgttccagag gtaccagagc ccgaaacccc gcccgccgat gaagacgtcc ttcatctcct
     7201 gcgtgacctt cttctcgctg accttcagcg tggagaggtc gatgtgcagc gtgcggttgg
     7261 tgtagccccg caccggcagg ctgggctcga actcgtgccg aaggagaacc ctgtgcgccg
     7321 ccttctggcg ggcaacgtcc tccgtgtacg aagtgcgggt cttgtagtcg tacgtggtcg
     7381 tcatggccac tccttcaggc tcgcggtctg ggcggtttcg gagagttcga gaaacggccg
     7441 ggctgtccgg ggcaaacctc cgcccggaaa cctgtggggg accgtagacc atgatctgcc
     7501 agaccgccgg accgaggcaa gcggtggagg gccgcccggg ctagcgcacc cgcgtccggc
     7561 tctgctcgcg gaagaactgc tggaggtagt gcgggccgcc ggtccccttg ccggtggacc
     7621 cgctcgcctt ccagccgccg aaggagttct gcccgggcca ggcgccggtc gtcgctcccg
     7681 cccgccggtt cgcgtagacc acgcccgcct ggataccctc gaagaacgtc tcgacctcgc
     7741 gctcgtcctc cgagaagatg cccgccgtca gcccgtaaga ggtccggttc gccaggtcca
     7801 gcgcctcgtc cagcgtcagg acgtcggcga cgacggtgag cgggacgaac agctcctcgc
     7861 ggaagagccg gtggtcgcgc ggcagaccgt cgatcacggt gggctcgacg tagtagccgt
     7921 gatcgaacag cccgcccgac aggcggcgtc cacccacgag aacccggcct ccgtcgcgct
     7981 tcgcctcctc gatcgcctgc tcgtagttgc ggacggcgcg ctcgttgatc accggcccca
     8041 tccagacgtc gcgctcgagg gggttgccga ccttgatggc cctggtcttc tcgacgagca
     8101 tctcgacgaa gcggtcgcgc acctggcggc tggcgtagac ccgcgagcag gccgagcact
     8161 tctggccgct cgcgccgaag gccgaccgca tcacgccgtc cgaggccttc tccgggtcgg
     8221 ccgacgccat gaccagcgcc gggttcttcc cccccatctc gatgatcagc ggccgcggca
     8281 cggggcgcgc cccgttctgc cgcagcagca tcatccctac ctccatcgac ccggtgaaca
     8341 cgaggccgtc caccccgtcg ttgttcgcga tctcctcgcc aacaggcacg ccgggaccgt
     8401 tcacgaggtt gaaggcgccg ggcggcagga actcggaggc gatctcggcc agcgggacgc
     8461 aggcgagcgg cgtgtcctcg gccggcttgc agacgacggt gttgccggcg agcagggccg
     8521 ccgccgtcgg ccccgccgcc agggcgagcg ggaagttgaa cggggcgatg acgccgaaca
     8581 cgccgtaggg ccgcagcacg ctcaggcttt cctcgcgcga gccgggcagc cccatcaggt
     8641 tggcgaagcc gtcggtctgc tccatccggt cgcagtagta gtcgaagaag tcggccgcct
     8701 cctcgacctc gccgacgcac tcgaggcggt tcttcccgac ctcgtagccg atgatcgccg
     8761 acagctcggc acgccgctcg cggatgcgct gccccatcct cctgacgatg cggacccgct
     8821 cttcccagcc aacgcggctc cactcgggga aggcccggcg ggcggccgcg atcgcctcgc
     8881 gggtctgctc gcgcgtcgcg gccgtgaagc ggccgatcag gatgcgggtg tcgatggggc
     8941 tgcggtcttc gaactcgccc ccctccccct ccacctggcg ccccccgacg agcatcgggt
     9001 gcgaccggcc gaagccggcc ctcacgcgct cgatcgcctc gtcgagatcg cggtgcaggt
     9061 ccgccatctg gtcggcgctc atcgtggcgt aggtgatctt cgcctgggcg ggtttcagct
     9121 cgctctgcgg catgcggggg cctcgtcagt tgttgagatt cttcgatgat accatgaggg
     9181 cccgatggcc tcccgcgccc gcctcttcgc catttcctgc ctcgcgatgt tcgtcttcgg
     9241 gatggtgatc gcactgctcg gcacgctctt cgggctgccg gaaaccaggg ccaggctggc
     9301 catcgacctc gcgcagcagg ggcagctgct gagcgtcatg ttcaccggcc tgctggccgc
     9361 caccatcgtc gccgggccga cgcaggaccg gttcggggcg aagctcgtgt tggtggcggc
     9421 ggcgggagcg gtcgcggtcg cgctggttct gctggccctc gcgcgaagct tctcgagcgc
     9481 ggccgtctgg gccgggctgc tggggatcgg cgccgggtgg ctgaacctcg gcgccaacgc
     9541 gctcgtgtcg gaggtctacc ccgagagccg cggccgcatg ctcaacctca ccaacgtctt
     9601 cttcggcgtc ggcgcgctgt tcgtcccgct gctcgtctcg gtcgcgttcg gcgt
//
LOCUS       DAOPQX010000115         9575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8064_length_9575_cov_4.495798, whole genome shotgun sequence.
ACCESSION   DAOPQX010000115 DAOPQX010000000
VERSION     DAOPQX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_9575_cov_4.495798"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2047)
                     /locus_tag="PKK95_07960"
     CDS             complement(<1..2047)
                     /locus_tag="PKK95_07960"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HOC18186.1"
                     /translation="MLLLLVAVLILFVWVARLQRRVTDLEAQSPWAPSAPPIQQTPPA
                     PRPIQQTPPPPPIPQAPPPPPPPAAAAPPPAPGGVHPPPPPAPPPAAPQPASPSSPAI
                     EWEALVGVKLFSWIAGIALALGGIFFLRYSIQSGWLQPPVRLGMGIVAGIVLLIACET
                     RRAGRYAVTAGALAAAGLVILFSSFYAAHALWSLIGPLPAFASLVAVAAVAVWLSLRH
                     DSLFIALLGLLGGFAAPALLSSGEDRPFTLFGYLLLLNAGLGLVAYRRRWAPLVALSL
                     VFTTLYQWGWVLSFLSPDRLGLALAIFTVFPLLAWGLLGVSARREDEGPESQERFRTM
                     AGANAALPLLLVLYLVMVPGYGERFPLVFGFLFVIDAALLVIARTRGPQELHALGAAT
                     TVLALLEWWGLNGVPAGRWPAVLGFVALFVVFYLWAHRQARHGGRPFTGLAERAALAA
                     PALLVLFPGLALTEPAFASPANPLAVLLGLTALVAVHSVEEEQALPYFLAAWLAVAWA
                     VIWSGRHVGPANWPATLAVYAVLALFFLAVPLAAARFRRGSALLPLAGFELLALAPQA
                     LLIPLAVQRWLPAQPWLLLATVAAIQVTASAASLAVRGGRLHAGATVLSALVLTAWMV
                     GAAPPAGRFVAMAGAILLAAFALAWREVSRWRRHPAQDGFDAAAAAALVLGQFVVALG
                     "
     gene            complement(2103..2366)
                     /locus_tag="PKK95_07965"
     CDS             complement(2103..2366)
                     /locus_tag="PKK95_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18187.1"
                     /translation="MPCLLALAALIAPRLVILLLWLFTGWFRGVFDTVLWPILGFVFL
                     PTTLLWYTAVQQWFGGNWTLWPIVGLVVALAIDVSPARGRRRD"
     gene            2495..2878
                     /locus_tag="PKK95_07970"
     CDS             2495..2878
                     /locus_tag="PKK95_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18188.1"
                     /translation="MPHRFVLVGFFALVALAALAACGGGTASGDLDKLQVTLSQFAVT
                     VTNTSGQVLTEVVAEINPTSPATPFKTRPETMGAGETRTLAHTSFMDRDSVPFSPRTA
                     KGRKVIVTAKDVDGTELKVELPFKL"
     gene            2919..3656
                     /locus_tag="PKK95_07975"
     CDS             2919..3656
                     /locus_tag="PKK95_07975"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOC18189.1"
                     /translation="MMTPLVPLVVACAVVSALAGPLQGQDMERPRLTVNPTPDFEVTG
                     LGDAAAWRQAEWTPMPRRQPDNHPYDTRFKVLYSDTGLYVLIEGTDRSLTATMTEDFM
                     DLWNEDVFEVFLWTDERYPVYFEYEISPLGRELPILVPNFGGEFLGWRPWHYEGERMI
                     RKATSIVGGPKEPKAAIEGWRAEFFIPYALLKPLQNVPPKPGTRWRANFYRMDYDGGK
                     RTTWQWAPVQGTFHQFQKYGELVFGGK"
     gene            complement(3724..5646)
                     /locus_tag="PKK95_07980"
     CDS             complement(3724..5646)
                     /locus_tag="PKK95_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOC18190.1"
                     /translation="MYSFHWLDIVVIVGFFLVIFGIAAYYSKKAGKDTGEFFLSGRDM
                     PWYIAGTAMVATTFAADTPLAVTELVARNGIAGNWLWWNLAIGGMLTVFFFARLWRRA
                     NIMTDVEFVELRYSGTAAAALRGFRAVYLGLFMNAIVLGWVNKAMEKIFKVTVPGVDP
                     FLLVVIAAVILAVYASASGLAGTARTDSFQFVFAMVGCIVLAVLVLRLPEVGGLVALK
                     GKLAPQVLDFLPRVGTVSAEGVTGGVLAMSAGAFLAYIGVQWWSAWYPGADPGGGGYV
                     AQRMMSAKDEKNSLFATLWFTIAHYTLRPWPWILVALAALVILPRAENAEMVRAENPA
                     LYEQVVAAYSNPATLRGGGLEAQTPEFRAMYEKYENTVDPGVMYPKLMVRYLPAGVLG
                     LLIAVFLAAYMSTLASQLNWGTSYLVHDFYRRFIRSDASEKHYVLVSRVSVVLMTVFS
                     LIVTRYFLTRISGAWEFVINASAGMGAVLILRWFWWRVNAWSEIAAMIAPLVIYPIAR
                     YGFDIQPPITLYPTVLGTTIVWLAVTFATRPTDDATLVKFYRRTYPGGAGWKRVAALA
                     PEVQGDTGFGRLFLNWGLGIVVVYSVLFGLGELLFGSGLVAALLLLLAVGAAWIIWRD
                     LKARGLGAETVPSQVR"
     gene            complement(5921..5995)
                     /locus_tag="PKK95_07985"
     tRNA            complement(5921..5995)
                     /locus_tag="PKK95_07985"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5960..5962),aa:Glu,seq:ttc)
     gene            complement(6247..6624)
                     /locus_tag="PKK95_07990"
     CDS             complement(6247..6624)
                     /locus_tag="PKK95_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOC18191.1"
                     /translation="MNYIGPSVVIKGVVSSGEDLTIAGRVEGDVHLDAGELMLAPGSS
                     VVGDLTVPSVIVHGSLQGNVIARVRIDVRPRASVSGSLATPALAVAEGAELHCRVEMP
                     AVSGTRAPQAPQAALPEKTAVAV"
     gene            6864..7424
                     /locus_tag="PKK95_07995"
     CDS             6864..7424
                     /locus_tag="PKK95_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18192.1"
                     /translation="MKKHAARMAVMALVALVLGSGVALAQVETCSAVGTWIMKVPSGG
                     ELIAVVTRGNLRTSGGQIEASWVVWEPTLASRFPAVRTTDPKGAWEEVGVVPMPARTN
                     FTWIAHGMGAFGSPLYVLRGRGYVTFQGCDQATFTTAMDLFLPSHNVWLDAPLVTLGP
                     EPWSATRMPVVPLADPNAPVTTGARR"
     gene            complement(7523..8263)
                     /locus_tag="PKK95_08000"
     CDS             complement(7523..8263)
                     /locus_tag="PKK95_08000"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC18193.1"
                     /translation="MITRILVSAAAVVIGLTASSPAQSQTPNTLTGAEKKQGWVLLFN
                     GVDFDGWRQCNGTAMPANWVIEDGAMKVFTAPGRKPGQGAGGDILYGVRKFRNFELSI
                     DWKTEKAGNSGIFYNVREVPGQPIYYAAPEVQVLDNVDATDNKIPSHLAGSLYDMLAA
                     DPKTVKPAGEWNTIVIRVENGKVTHTQNGSKVVEYTLWTPEWDQLVENSKFKTFPGFT
                     EGIAREGYIGLQDHGYAIWFRNVKIREL"
     gene            8700..9467
                     /locus_tag="PKK95_08005"
     CDS             8700..9467
                     /locus_tag="PKK95_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC18194.1"
                     /translation="MSEFQRVRVVLLAHWPVVRAGLRRLLEHHEDVRVVGEFGAIDEV
                     LAAREHCDVIVVDPDCEEISLRAVSVLAEGCESRILVFTSSGDARVCTRAIELGASGV
                     VSKDASASVVVRAVLKVHAGELWLDRAKTAAVLSRAIRRRHDPETLKIASLTKREREI
                     VALVGEGLRNSAIAERLFISEATVRNHLTSILGKLELADRFDLAVYAFRHELIPPPEA
                     VRVAPAAADASARPRPVPVPIARAATQYVARPGGVNG"
BASE COUNT         1646 a         3196 c         3424 g         1309 t
ORIGIN      
        1 agccgagggc caccacgaac tgcccgagca cgagtgcggc ggccgcagcc gcgtcgaaac
       61 cgtcctgtgc cgggtgacgc cgccaccgcg acacctcacg ccaggcgagc gcgaacgcgg
      121 ccagcagaat ggcgccagcc atcgcgacga acctgcccgc cggcggggca gcgccaacca
      181 tccacgcggt cagcacgagc gccgagagga ccgtcgcccc ggcgtgcagc ctgcctccgc
      241 gtacggccag tgacgcggca gacgcggtca cctggatcgc ggccacggtg gcgagcagca
      301 accacggctg cgcgggcagc caccgctgca cggccagtgg gatcagcaac gcctggggcg
      361 cgagcgccag gagttcgaaa ccggccagcg gcaagagtgc gctgccgcgc ctgaagcgcg
      421 cggccgcgag cggcaccgcg aggaagaaca gcgccagcac ggcgtagacc gcgagcgtcg
      481 ccggccagtt cgcgggccca acgtgacggc cggaccagat cacggcccag gcgacggcga
      541 gccaggcggc caggaagtac ggaagggctt gttcctcttc gaccgagtgc acggcgacga
      601 gcgcggtcag gccgagcagc accgcgaggg gattggccgg cgaggcgaac gcgggctcgg
      661 tgagggcgag accgggaaac aggacgagca gtgccggcgc cgcgagcgct gcgcgttcgg
      721 ccaggcccgt gaacgggcgg ccgccgtgcc gcgcctggcg gtgcgcccac aggtagaaca
      781 cgacgaagag ggcgacgaac cccagcaccg ccggccagcg cccggcgggg acgccgttga
      841 gaccccacca ctccagcagt gcgagcaccg tcgtcgcggc gcccagcgcg tgcagctcct
      901 gcggccctcg cgtccgggcg atgacgagca gggcggcgtc gatcacgaac aggaacccga
      961 acaccagcgg gaagcgctcg ccgtagcccg ggaccatcac gaggtagagc acgagcagca
     1021 ggggcagggc ggcgttggcg cccgccatcg tgcggaagcg ttcctggctc tccggccctt
     1081 cgtcttcgcg tcgagcgctc accccgagca ggccccacgc gagcagcggg aagacagtga
     1141 agatggcgag ggccagcccc aggcggtcgg gcgacaggaa cgagagtacc cagccccact
     1201 ggtagagcgt cgtgaagacg aggctcaggg ccaccagcgg cgcccagcgg cggcggtagg
     1261 cgacgaggcc cagaccggcg ttgagcagca gcaggtagcc gaagagcgtg aagggacggt
     1321 cctcgcccga cgacagcagg gcaggcgccg cgaacccgcc cagcaggccg agcagcgcaa
     1381 tgaagagcga gtcgtgccgc agcgacagcc acaccgccac cgcggccact gcgacgagcg
     1441 acgcgaaggc cggcaacggc ccgatcagcg accagagcgc gtgtgccgcg tagaacgacg
     1501 agaagaggat cacgagcccg gcagcggcaa gggcgccggc ggtgaccgcg tatcgcccgg
     1561 cacggcgcgt ctcgcaggcg atgaggagca cgatccccgc gacgatcccc atcccgaggc
     1621 gaacgggcgg ctgcagccac ccgctctgga tcgagtagcg gaggaagaag atgccgccaa
     1681 gcgccagggc aatccccgcg atccaggaga acagcttcac cccgacgagc gcctcccact
     1741 cgatcgcggg ggaggacggc gaggctggtt gtggcgccgc gggcggggga gcagggggtg
     1801 gtggcgggtg gacgcccccc ggcgccggcg gtggagcagc cgcggcaggc ggcggtggag
     1861 gaggcggcgc ctgcgggatc ggtggaggag gcggtgtctg ctggatcggg cgaggagccg
     1921 ggggcgtctg ctgaatcggg ggagcggaag gcgcccaggg gctctgcgcc tcgaggtccg
     1981 tcacgcgacg ctgaagccgc gcaacccaga cgaacaggat cagcaccgcg acgagcagca
     2041 ggagcatgcg cggccggatc ctaacacgcc tgcagaagca gctgcgtgtc agtggcggcg
     2101 cgtcagtcgc gccggcggcc tcgcgcgggc gacacgtcga ttgcgagcgc gacgaccagg
     2161 cccacgatgg gccagagcgt ccagttcccc ccgaaccact gctgcacggc cgtgtaccac
     2221 agcagggtgg tgggcaggaa gacgaagccc aggatgggcc agagcacggt gtcgaagacg
     2281 ccccggaacc agccggtaaa cagccacagc aagaggatca ccagccgtgg ggcaatgagc
     2341 gcggccaggg cgaggagaca gggcatcggg ggaggatacc gcggaacaag gcctgccgcg
     2401 ccgtagtggc acaggcgtcc cgcctgtggg caccctcagg gcgcttgact cagcaggcgc
     2461 ggttgcctat cgttccgggt caaggaggac ctccatgcca caccggttcg tcctcgtggg
     2521 tttcttcgcg ctggtggcgc tcgcggcgct cgccgcctgc ggcggcggga cggcatccgg
     2581 cgacctcgac aagctgcagg tgaccctgtc gcagttcgcg gtcacggtga ccaacacctc
     2641 gggacaggtg ctgaccgaag tggtggcgga gatcaacccg acctcgccgg ccactccctt
     2701 caagacccgc cccgagacga tgggtgccgg ggagacgcgg acgctggccc acaccagctt
     2761 catggaccgc gactcggtgc cgttcagccc tcgcacggca aaggggagga aggtcatcgt
     2821 gaccgcgaag gacgtggacg gcacagagct caaggtggag ctgcccttca agctgtgatc
     2881 gagcggggcg aggacgatcg cgccgcgccg ggaggagaat gatgacgccc ctcgtgcccc
     2941 tcgtggtggc ctgcgccgtc gtgtcggcgc ttgcgggccc ccttcaagga caggacatgg
     3001 aacggccgcg actgacggtg aacccgaccc ccgacttcga ggtgaccggc ctcggcgacg
     3061 ccgccgcctg gcggcaggcc gagtggacgc cgatgccaag gcggcagccc gacaaccacc
     3121 cctacgacac gcgcttcaag gtgctctact cggacacggg gctgtacgtc ctcatcgagg
     3181 gcaccgaccg ctcgctcacg gccaccatga ccgaggactt catggatctg tggaacgagg
     3241 acgtgttcga ggtcttcctc tggaccgacg agcgctaccc ggtgtacttc gagtacgaga
     3301 tctcgccgct cgggcgggag ctgccaatcc tcgtgccgaa cttcggcggc gagttcctgg
     3361 gctggcggcc gtggcactac gagggcgagc ggatgatccg caaggcgacc tcgatcgtcg
     3421 gcgggccgaa ggagccgaag gcggcgatcg aggggtggcg ggccgagttc ttcatcccgt
     3481 acgcgctgtt gaagccgttg cagaacgtgc cgccgaagcc tggcacccgc tggcgtgcga
     3541 acttctaccg gatggactac gacggcggga agcgcaccac ctggcagtgg gcgccggtgc
     3601 agggcacgtt ccaccagttc cagaagtacg gcgagctggt gttcggcggg aagtagaagt
     3661 aaggcctgga ggctgacggc ccgcgccggc gggccgtcag cctcccaggg gtcgtggtgc
     3721 cgctcagcga acctgcgacg gaaccgtttc cgcccccagc ccgcgcgcct tcaggtctct
     3781 ccagatgatc caggcggccc cgacggcgag cagcagcagc agcgccgcca ccagcccgct
     3841 gccgaacagc agctcgccga ggccgaagag aaccgagtac acgacaacga tccccaggcc
     3901 ccagttgagg aacagccgcc cgaagccggt gtcgccctgg acctcgggtg cgagggccgc
     3961 cacgcgcttc cagccggcgc cgccggggta cgtgcgccgg tagaacttca ccagcgtcgc
     4021 gtcgtcggtc ggccgggtcg cgaaggtgac cgccaaccac acgatcgtgg tgccgagcac
     4081 cgtcgggtac agcgtgatcg gaggctggat gtcgaacccg taccgggcga tcggatagat
     4141 cacgagcggc gcgatcatcg ccgcgatctc cgaccacgcg ttcacccgcc accagaacca
     4201 gcggaggatg agcaccgcgc ccatcccggc gctggcgttg atgacgaact cccaggcgcc
     4261 agagatccgg gtcaggaagt agcgcgtgac gatcagcgag aacaccgtca tcaggacgac
     4321 gctcacccgg gaaaccagga cgtagtgctt ctccgaggcg tcggagcgga tgaaccgcct
     4381 gtagaagtcg tggaccaggt aggaggtgcc ccagttgagc tgtgaggcga gcgtggacat
     4441 gtacgccgcc aggaagaccg cgatcagcag cccgagcacg cctgccggca ggtagcgcac
     4501 catcagcttg ggatacatca cgccgggatc gaccgtgttc tcgtacttct cgtacatcgc
     4561 cctgaactcg ggcgtctggg cttcgagacc cccgcccctg agcgtggcgg ggttcgagta
     4621 ggcggccacc acctgctcgt agagggccgg gttctcggcg cggaccattt cggcgttctc
     4681 ggctcgcggg aggatcacca gcgccgccag cgcgacgagg atccagggcc agggacgcag
     4741 cgtgtagtgc gcgatcgtga accacagggt ggcgaagagg ctgttcttct cgtccttggc
     4801 cgacatcatg cgctgggcga cgtagccgcc gccgcccgga tccgctcccg ggtaccacgc
     4861 ggaccaccac tggacgccga tgtacgcgag gaacgccccc gccgacatcg cgaggacgcc
     4921 gcctgtgacc ccctcggcgc tcaccgttcc caccctcgga aggaagtcga gcacctgcgg
     4981 cgcgagcttg cccttcaggg cgacgagccc tccgacctcg ggcagccgca ggacgaggac
     5041 cgccaggacg atgcagccca ccatggcgaa aacgaactgg aagctgtcgg tgcgggccgt
     5101 gccggccagg ccggacgccg acgcgtacac tgccaggatg acggccgcga tcaccacgag
     5161 caggaagggg tcgacgccgg gaacggtgac cttgaagatc ttctccatcg ccttgttgac
     5221 ccacccgagg acgatggcgt tcatgaagag gccgaggtag acggcgcgga acccgcggag
     5281 ggcggcggcg gccgtgcccg agtagcggag ctcgacgaac tccacgtcgg tcatgatgtt
     5341 ggcacgccgc cagagcctgg cgaagaagaa gaccgtcagc atccccccga tggccaggtt
     5401 ccaccagagc cagttgccgg cgatgccgtt gcgcgcgacc agctcggtga cggccagggg
     5461 cgtgtcggcg gcgaaggtgg tggccaccat cgccgtgccc gcgatgtacc acggcatgtc
     5521 gcggcccgac aggaagaact cgccggtgtc cttgcccgcc ttcttcgagt agtaggccgc
     5581 gatgccgaaa atcaccagga agaacccgac gatgacgacg atgtccagcc agtggaaact
     5641 gtacatgatg aattgtccga gcgtgaaagg ttcaccaggc ggccggcctt cggctcgccc
     5701 ctggggacgg gacgctattc tataccgctc accgttttgg cgtgaaacgg cccgagactg
     5761 ggaagggaag ggcccgtgcc tgcgaaccga gaactccgct ggcctggcgc ttgaggggct
     5821 tcgaatcgat ggcgaacaag atccagcccg cttcgcgtgt cagctggctt gccagccgta
     5881 gctcaccgag gaggaaggca gtcggaagtg agcggaggca tggcgtcccc agcgggagtc
     5941 gaacccgcgt ttcggccttg aaagggccgc gtcctgacca ctggacgatg gggacgggcg
     6001 agaacatttc atcctagcac agcatgggcc ccggcaactc ggcgtgtaga ccgtgagggc
     6061 tcgagtaggt ggggcgacgt ctgcagagcg tccgcgcagg gcgcggaagg gcagggcagc
     6121 gacgactggg atttcgatcc gtcgatcgga atcccgcgat ccagggcggg actggagctg
     6181 ggatcggatt cgggcggcaa gcaccggggg ggcgtgacgg ggacgcgcgg tgcgggaacg
     6241 aaagcgtcag acggcgacgg ccgtcttctc cgggagcgcg gcctgcggtg cctgcggcgc
     6301 gcgcgtgccg ctgaccgccg gcatctcgac gcggcagtgc agttcggcgc cctccgccac
     6361 ggccagcgcg ggggtggcga ggctgcccga gaccgaggcg cgcggccgca cgtcgatgcg
     6421 cacccgggcg atgacgttgc cctgcaggct cccgtggacg atgaccgacg gaacggtgag
     6481 atcgccgaca accgacgacc cgggcgcgag catcagctct cccgcgtcca ggtggacgtc
     6541 gccctcgacc cgtccggcaa tggtcaggtc ctcgcccgac gacaccactc ccttgatgac
     6601 gaccgatgga ccgatgtagt tcatcgcacc tccggcgggg cgctgggcaa ggaccatacc
     6661 gcacgaaatg tgcccgttgt tcgcgccccc ggtttcgatc gggccgggca ttccgcacga
     6721 catcgtgatc gaatcaccga tttgtcggaa ggccgcaggt gggcgagggt tgacaaaaac
     6781 agcggttccc ccttgacagc acggcaggct gaggcagact gtaccggtcc aggttgtagc
     6841 ccccttcggc gggaggagca ctcatgaaga agcacgcggc caggatggca gtgatggcgc
     6901 tggtcgccct ggtcctgggg agcggggtgg ccctggccca ggtcgaaacc tgctcggccg
     6961 tcggcacctg gatcatgaag gtgccgagcg ggggcgagct gatcgcggtg gtgacccgcg
     7021 gaaacctgag aacgtcaggc ggccagatcg aggcgagctg ggtggtctgg gaaccgaccc
     7081 tcgccagccg gttcccagcc gtgcgaacca ccgatcccaa gggcgcgtgg gaggaggtcg
     7141 gggtggtgcc gatgcccgcc cgcaccaact tcacctggat tgcgcacggg atgggcgcct
     7201 tcggctcgcc gctgtacgtg ctgcgcgggc gcggctacgt caccttccag ggatgcgacc
     7261 aggccacctt caccacggcg atggacctgt tcctgccctc ccacaacgtg tggctggatg
     7321 cgccgctggt gacgctcggc ccggaaccgt ggagcgccac gcggatgccg gtcgtgccgc
     7381 tcgccgaccc gaacgcgccg gtcacgaccg gcgcgcggcg ctagcctctc gagcgcgatt
     7441 cgatgcgcgg gcggtcagcc gcccgcgccc cttctccgcg cgcccacggc gcggccgccc
     7501 ctgccggccc cgatcccgct gctcacagct cccggatctt gacgttgcgg aaccagatgg
     7561 cgtagccgtg gtcctggagg ccgatgtacc cctccctggc gatgccctcg gtgaagccgg
     7621 ggaacgtctt gaacttgctg ttctcgacca gctggtccca ctcgggcgtc cagagcgtgt
     7681 actcgaccac cttcgagccg ttctgcgtgt gggtgacctt gccgttctcc acccggatga
     7741 cgatcgtgtt ccactcgccc gcgggcttca cggtcttggg gtcggccgcc agcatgtcgt
     7801 acagcgaacc ggcgaggtgg ctcgggatct tgttgtcggt ggcatccacg ttgtcgagca
     7861 cctgcacttc gggcgccgcg tagtagatcg gctggcccgg cacctcacgg acgttgtaga
     7921 agatccctga gttgccggcc ttctcggtct tccagtcgat cgacagctcg aagttccgga
     7981 acttccgcac gccgtagagg atgtcgccgc cggcgccctg gccgggcttc cggcccgggg
     8041 ccgtgaacac cttcatcgcc ccgtcctcga tcacccagtt ggcgggcatg gcggtgccgt
     8101 tgcactgccg ccacccgtcg aagtcgaccc cgttgaacag cagcacccac ccctgcttct
     8161 tctccgcgcc ggtgagcgtg ttgggcgtct gcgattgggc ggggctgctg gcggtgaggc
     8221 cgatcacgac ggccgccgcc gaaaccagga tgcgcgtgat catcgatccg ttcctccgtg
     8281 gcgaagtccg aaggccatag cttacaggat cctcctggga gccgggcgga gagcaggtag
     8341 gaccaggatc ctgcttcccg ttcgagtgca ttgatagcgg actagtcgag tcttgatttc
     8401 cgtcagcccc gaacccggca gaatccctgc cgcggatgat ccgaacgtgc gctctcgctg
     8461 ccgccggccg cgcgttgcga gcggccaacg gtgcgaatct tgccgcaatc tggccggaaa
     8521 cggtgctccc agccctccca ctcgattccg cctgccgctg ttgggacgca cgtcctacgg
     8581 agtgtggcgt gaatggtgca gagagggcgc gcgagatatc acaagctgaa cctatttccg
     8641 cgagacgtag cggccggacg gccgcgacgg acgtgcaagc cgacaggggg caagagtcga
     8701 tgtctgaatt tcagagggtg cgggtcgtgc tgctggcaca ttggcccgtg gtgcgtgccg
     8761 gtctccggag gctgctcgaa catcacgagg acgtgagggt ggttggcgag ttcggggcga
     8821 tcgacgaggt gctggcggca cgagagcact gtgacgtcat cgtcgtcgat ccagactgcg
     8881 aggagatctc gctgcgggcc gtgtcggtgc ttgccgaggg ctgcgagagc cggatcctgg
     8941 tgttcacctc gtcgggcgac gcgcgagtct gcacgcgggc catcgagctg ggcgcctcgg
     9001 gcgtggtctc gaaggacgcc tcggcttcgg tcgtcgtccg ggccgtgttg aaggtgcacg
     9061 ccggtgagct gtggctcgac cgggcgaaga cggcggccgt cctctctcgc gccatccgcc
     9121 ggcgccacga cccggagacg ctgaagattg ccagcctgac caagcgggag cgcgagattg
     9181 tcgccctggt gggggagggg ctgcgcaaca gcgcgatcgc cgagcggctg ttcatcagcg
     9241 aagcgaccgt gcgcaatcac ctgacgtcga tcctgggcaa gctcgaactc gccgaccgct
     9301 tcgacctggc ggtgtacgcg ttccgccacg agctgatccc gccgcccgag gcggtgcgcg
     9361 ttgcgccggc ggccgccgac gcttcggccc ggccgcggcc cgttccggtt ccaatcgccc
     9421 gcgcggcgac ccagtacgtg gcgcgtccgg gcggcgtcaa cgggtgaggc ggacatgtac
     9481 gagcagttct acggcttgct cgagcgcccc ttcgatctga cgccgaaccc gcggtacctc
     9541 gtcctcacac cgtcccatga ggaggccctc gggaa
//
LOCUS       DAOPQX010000116         9558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8077_length_9558_cov_4.775018, whole genome shotgun sequence.
ACCESSION   DAOPQX010000116 DAOPQX010000000
VERSION     DAOPQX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9558
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8077_length_9558_cov_4.775018"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1109
                     /locus_tag="PKK95_08010"
     CDS             276..1109
                     /locus_tag="PKK95_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOC18195.1"
                     /translation="MKRLTSRHHPLVAACRAIARGGDTGGRMLLDGPHLVEEALGSAV
                     EIEAAVLDAAFAASEEGAAILHGLAGAGIDALTAARPVLEAMSPVTHPSGIVAIARRP
                     PTEMARALGGALPLAVVGVDVQDPGNVGAIVRAAEAGGATGAIFCGASADPLGWKALR
                     GSMGSALRLPLAVASSAGEAIAAARRAGLRVVAAVVRGGDLLHESDFTPPTALLVGGE
                     GGGLGPEVIAAADAAVVIPMTPPVESLNVSVAAALLVYEARRQRVLAAARPKGTGRQA
                     T"
     gene            1106..2482
                     /locus_tag="PKK95_08015"
     CDS             1106..2482
                     /locus_tag="PKK95_08015"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOC18196.1"
                     /translation="MSTGRLFEDEPREEGGGRSLRPPLADRVRPRGFDELVGQEELLA
                     PGRPLRVAIERDLLQSIILWGPPGTGKTTLARLVAQVTRAHFISFSAVLSGIKEIKAV
                     MAEAEQARRQLGRRTIVFVDEIHRFNKAQQDAFLPRVEAGDIVLIGATTENPSFEVNS
                     ALLSRSQVFVLKPLDQPAVLGILRRALGDAERGLGAEEVDVDEDALEAMARLANGDAR
                     AGLNLLEATVTAAPADPASGRRRVSVAFVEQAVQRKALLYDKAGEEHFNLISALHKSM
                     RNSDPDAAVYWLARMLEAGEDPLYIARRVVRFASEDIGIADPQALPVAVAAKDAVHFI
                     GMPEGNTALAQAVIYMATAPKSNAVYVAYQRASDAALKDEAEPVPLHLRNAPTRLMKS
                     LDYGKGYRYAHDEADAVTGMDCLPERLQGRRFYRPTDRGFEKEIHRRLEAWAAIKAKR
                     KGEGSPET"
     gene            complement(2511..3461)
                     /locus_tag="PKK95_08020"
     CDS             complement(2511..3461)
                     /locus_tag="PKK95_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOC18197.1"
                     /translation="MSVRRNLFDIVGAVVAAMLLFSLGSYTARQGAEQQLASRDRQIA
                     SLEREMGEISRQLKQEQTTPSGTAGLAEQQPAVTQATVVEEVKRQLQAEMGLLPVQLL
                     RDRRKSFVELYAYDNNGRSNYGTAGYLGNGYFVTVKHAVVALHPGEDDGRRIVSVKLR
                     INRKDVLATVVDAGDARTEVDPGDWAILRVPRPVNLPPLRVNLGFSYDFADPIFRLGN
                     DYSKGIILSTGYIGQRTANDLVTCLTDGHPGVSGGGVLDQRGELVGIPIGRMQGDYRF
                     SFILPLRKEIFRKVPTLHVSVPPVEAPTVAVTVPPTAAPQ"
     gene            complement(3741..5516)
                     /gene="mutL"
                     /locus_tag="PKK95_08025"
     CDS             complement(3741..5516)
                     /gene="mutL"
                     /locus_tag="PKK95_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOC18198.1"
                     /translation="MGRIHRLSAELANQIAAGEVVERPSSVVKELVENAIDAGASRLA
                     ITVEQGGKRLIRVEDDGEGMSPEDAELALARHATSKIASAEDLAAILTHGFRGEALPA
                     IASVSHFVLRTRAADAIAGTEIRVNAGTLASVREVAAPPGTSVEVADLFYNLPARRKF
                     LKADGAETAQVSRTATQLALAYPEVGFTLTSGGRMLFQCPPVRSRRDRFFQLYGDRPD
                     LVEVAKEAAGISVVGLVASLTDQGPKRGPQNVFVNRRIVKDRTIAHAIVDAYSVATVK
                     ERSPEVHLFLEMPPEEVDVNVHPTKAEVRFRNQSLVHEVVKRAISAALGRTSAPELQL
                     QPPPAFEPAPSAQAFPGLIAGVFPSRWVPPARADEGPFPGLPQAPQVPVASPSGAAAP
                     SPDAPWGLRPLIPLGQFRDTFIIAVDDEGISIIDQHVAHERVLFERVMERLTAGRLES
                     QRLLEPLVLDLPSAAHDVLLARQAELDRLGFELASFGGRSVRVAAVPALLSPDESVAA
                     LREMAEDLEGLDRGGKVGEGLQRIAALTACHAAVKANCPLTHEKMVHILDELRKTAYS
                     SICPHGRPVMLRLTRREVERNFQRI"
     gene            complement(5638..8469)
                     /gene="ppdK"
                     /locus_tag="PKK95_08030"
     CDS             complement(5638..8469)
                     /gene="ppdK"
                     /locus_tag="PKK95_08030"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOC18199.1"
                     /translation="MAKKMVKAKKQAAGRRTAKTTARAARPAARGVRTQGKAAKKATK
                     YVYFFGGGKADGNRTMKDLLGGKGSGLAEMTNAGLPVPPGFTITTAACNLYYERGRRV
                     PPEVDREMEANLAKLEKVAGQQFGSVKNPLLVSVRSGAKFSMPGMMDTILNLGLNDET
                     VEGLKARTGNGRFAYDSYRRFMQMFGNVVLEIPKEEFEKEFDAVKHERGAKVDTDLDE
                     DALREVVKRYKGVVREKTGRSFPIDPMEQLRGARDAVFRSWNNPRAKEYRRIYEIPDH
                     IGTAVNVQLMVFGNSGERSATGVGFTRNPATGKKEFYGEFLINAQGEDVVAGIRTPMP
                     IAQLEEVMPQAYRELRRHTTLLEKHYKDVQDFEFTIENEKLYMLQTRSGKRTGYAAVV
                     IATDMVAEKLLKPEEALLLVDPSSLSQLLAPVFDPKEWKGIPVSTKGLPASPGAASGQ
                     IVFTADEAVKWTEQGKKVVLVRKETVPDDIHGMFVSQGVLTATGGMTSHAAVVGRQMG
                     KPAVVGAHELRVDEEGKKLAIGGRTLREGDYIAFDGLSGEVKVEKVATKPSEILQVIN
                     GTMKPEESEIYQRFERLLGWADKKRTMGIRANADLPDQANIAYAFGARGIGLCRTEHM
                     FFEGDRLPIVRRMILADNEADRRAALAELLPLQRGDFYGVFKAMNGTPVTVRTIDPPL
                     HEFLPKREELMVDIAVLEAKNGNARELEEKRALLARVEELHEFNPMLGHRGCRLGIAY
                     PEITEMQARAIIEAACQLNKEGLKVVPEIMIPLVGHVKEFQDQKAVVDRVAQDVMKEQ
                     GVKIKYLVGTMIEVPRGAVTAGDIAREAQFFSFGTNDLTQMTFGFSRDDAGKFLKIYQ
                     EKRILDGDPFASIDTTGVGLLVSMGATAGRATRSDLKLGVCGEHGGDPASIHFFQKVG
                     LNYVSCSPFRVPVARLAAAQAAMAVKVGD"
     gene            complement(8506..>9558)
                     /locus_tag="PKK95_08035"
     CDS             complement(8506..>9558)
                     /locus_tag="PKK95_08035"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOC18200.1"
                     /translation="GWVAREVFGEGARAEEAALAGRLAKADLVTDMVRELTELQGTMG
                     GIYAREEGLPEAVWRAIYNQYLPASVESDAPPSRADLGPAALPWAALALADKLDTIVG
                     LFAAGERPTGTRDPMGLRRQAHGVMKILVDLRELTGLDAAVGLEAMLERARAEYAEGC
                     GGGPALPEENQAERRDRAVFLEERLRFLFQQRGFGYDEVNAVAGARRSFLDAPFDARL
                     RLEALQRVRALPEFEALAVAFKRVKNLARELPGEPSPSTGRLTEPAERGLLQEYETRG
                     AAVRAAASRREYFEAFRLAAGFRERLDRFFTEVFVMVEDEGLRTERLSLVWRLHDLML
                     DLADISEIVPRSEPNR"
BASE COUNT         1376 a         3403 c         3277 g         1502 t
ORIGIN      
        1 gcgcctcgac cagccgctgg cgctccttgg ccgcctggta ctccgcgttc tcgctgagag
       61 cgccgagctc gcgcgcccgc ttgatctcct tcggcagctc catccgaagc tcgcgctcga
      121 gcccctgtat ttcatcctcg aaccgcttga ccaggctgtc cttgatcgtc atcgcccgtg
      181 cacgcgctcc cttccacccg aaccgcgaga aaactctaga gtttcctggc gatcttagca
      241 cagtctgcta tgctggtcgg cgcccgcgag agttcatgaa gcgcctgacc agccgccatc
      301 acccgctcgt cgccgcctgc cgcgcgatcg cgcgcggggg cgacacgggc ggccgcatgc
      361 tgctggacgg accccacctg gttgaggaag cgctcggcag cgccgtcgag atcgaagccg
      421 cagtgctcga cgccgccttc gcggcgtcgg aggaaggcgc ggccatcctc cacggcctgg
      481 cgggcgccgg catcgacgcc ctgaccgcgg cgcggcccgt cctcgaggcc atgagtcccg
      541 tgacccaccc gtccggcatc gtcgccattg ccaggcgtcc gccgaccgag atggcccgtg
      601 cccttggcgg cgccctcccc ctggccgtcg tcggcgtgga cgtgcaggac ccgggcaacg
      661 tcggggcgat cgtgcgggcc gccgaggcgg gcggcgcgac cggcgcaatc ttctgcggcg
      721 cgagcgccga cccgctgggc tggaaggcgc ttcgcgggtc gatgggaagc gccctgcggc
      781 tgccgctggc cgtggcctcg tcggccggcg aggcgattgc ggcagccagg agggcgggct
      841 tgcgcgtggt cgctgccgtc gtgcgcggag gcgacctgtt gcacgagagc gacttcacgc
      901 ccccgaccgc cctgctggtc ggcggcgagg gcggcggact cgggcccgag gtgatcgccg
      961 cagccgacgc cgccgtcgtc atcccgatga ctcccccggt cgagtcgctg aacgtgtcgg
     1021 tggccgcggc gctgctcgtg tacgaggcgc gccgccagcg cgtcctggcc gcggcgcggc
     1081 cgaagggcac cgggaggcag gcgacgtgag caccggacgg ctgttcgagg acgaaccccg
     1141 cgaggagggg ggcgggcgga gcctgaggcc gccgctggcc gaccgcgtgc ggccgcgcgg
     1201 cttcgacgaa ctggtcggcc aggaggagct gctcgccccc ggccggccgc tccgcgtggc
     1261 gatcgagcgc gacctgctgc agtcgatcat cctgtggggg ccgcccggga cgggcaagac
     1321 gacgcttgcc cggctggtgg cacaggtgac gcgcgcgcat ttcatctcct tcagcgccgt
     1381 cctctcgggc atcaaggaga tcaaggcggt gatggccgag gccgagcagg cgcgccgcca
     1441 gctcgggcgg cgcacgatcg tcttcgtgga cgagatccac cgcttcaaca aggcccagca
     1501 ggacgcgttc ctgccgcgcg tcgaagctgg cgacatcgtg ctcatcgggg ccaccaccga
     1561 gaacccttcc ttcgaggtga actccgcact cctctcgcgc tcgcaggtat tcgtgctcaa
     1621 gccgctggac cagccggccg tcctcggcat cctcagacgg gccttgggcg acgccgagcg
     1681 gggactcgga gccgaagagg tggacgtgga cgaggacgcg ctcgaggcga tggcgcgcct
     1741 cgcgaacggc gacgcgcggg cgggactcaa cctgctcgag gcaaccgtga ccgccgcccc
     1801 ggccgacccc gcgtcggggc gccggcgagt gagcgtggcg ttcgtcgagc aggccgtgca
     1861 gcgcaaggcg ctcctctacg acaaggcggg cgaggagcac ttcaacctga tctcggcgct
     1921 gcacaagtcg atgcgcaaca gcgaccccga cgccgccgtg tactggctgg cgcggatgct
     1981 cgaggccggc gaggaccccc tctacatcgc gcgccgcgtc gtgcgcttcg cctccgagga
     2041 catcgggatc gccgacccgc aggcgctgcc cgtcgccgtc gcggccaagg atgccgtcca
     2101 cttcattggg atgcccgagg ggaacaccgc gctggcgcaa gcggtgatct acatggccac
     2161 ggcgccgaag agcaacgccg tgtacgtggc ctaccagcgc gcttccgacg ccgccctcaa
     2221 ggacgaggcc gagccggtgc cgctgcacct gaggaacgca cccacccgct tgatgaagtc
     2281 gctcgactac gggaagggat accgctacgc gcacgacgag gccgacgcgg tgaccggcat
     2341 ggactgcctg ccggagcggt tgcagggccg ccgcttctac cgcccgaccg accgcggctt
     2401 cgagaaggag atacaccgcc gcctcgaggc ctgggcggcc atcaaggcga agcggaaggg
     2461 agagggaagt cccgaaacct gagttctgag ccctgggtct cgcgccttcc tcactgcggt
     2521 gccgcagtcg gcgggaccgt caccgcgacc gtgggcgcct ccacgggcgg caccgacacg
     2581 tgcagcgtgg gtaccttgcg gaagatctcc ttccgcagcg gcaggatgaa cgagaaacgg
     2641 tagtccccct gcattcgccc gatcgggatc cccacgagct caccgcgctg atccagcact
     2701 ccgccgcccg aaacgcccgg gtgcccgtcg gtgaggcagg tgaccaggtc gttcgccgtc
     2761 cgctggccga tgtagccggt gctgaggatg atgcccttgg agtagtcgtt gcccagcctg
     2821 aagatgggat ccgcgaagtc gtacgagaaa ccgaggttca cccgcagcgg cggaaggttc
     2881 accggccttg gcacccgcag gatcgcccag tcgccggggt ccacctcggt gcgcgcgtcg
     2941 cccgcgtcca ccaccgtcgc cagcacgtcc ttccggttga tgcgcagctt caccgaaacg
     3001 atccgccggc cgtcgtcctc gcccgggtgg agcgccacga cggcatgctt cacggtgacg
     3061 aagtagccgt tcccgaggta gccggccgtc ccgtagttgc tccggccgtt gttgtcgtat
     3121 gcgtacagct cgacgaagct cttgcgccgg tcgcgcagga gctgcaccgg cagcagcccc
     3181 atctcggcct gcagctgacg cttgacctcc tcgaccaccg tcgcctgggt gaccgccggc
     3241 tgctgctcgg caagccccgc cgtgcccgac ggggtggtct gctcctgctt gagctggcgc
     3301 gagatctcgc ccatctcgcg ctcgaggctg gcaatctggc ggtcgcggct ggcaagctgc
     3361 tgttcggcgc cctggcgggc cgtgtagctg ccgagagaga acaggagcat ggcagcgacc
     3421 actgccccca caatgtcgaa gaggttacgt cgaacggaca tccggctctc cggtccgcgg
     3481 ccgttgactc aggcgggttc gagcggcccg cgggccgccg gttcctccaa gttcggcagc
     3541 taaatcaccc cgtgcggggc ttacagcttt gccgcaccgg tgcggggcac cgggcaaccc
     3601 tttcgcttgg aatggatgag gtaagtctca gactgcaggc gacttacaca cctgacgact
     3661 tacgtatcat cactgtaagc gggccgcggc aggggtgtca aactgagcag tattcagatt
     3721 cgggattcgg gatttgggat tcagatcctc tgaaagttcc tctccacttc ccgtctcgtc
     3781 aggcgcagca tcacgggccg gccgtgcggg cagatcgacg agtaggccgt ctttcgaagc
     3841 tcgtccagga tgtgaaccat cttctcgtgg gtcagggggc agttggcctt gaccgccgca
     3901 tggcatgcgg tgagtgcggc aatccgctgg agcccctccc ccaccttgcc gccccgatcg
     3961 agcccttcga ggtcttcggc catttcgcgc agcgcggcga cgctctcgtc ggggctgagc
     4021 agcgcgggca ccgccgccac ccgcacgctg cggccgccaa aggacgccag ctcgaacccc
     4081 aaccggtcga gttcggcctg tcgcgccagg agcacgtcgt gggcagccga cggcagatcg
     4141 agcaccaggg gctccaacag gcgctggctc tccagccgcc ccgcggtcaa ccgctccatc
     4201 acgcgctcga acagcacgcg ttcgtgcgcg acgtgctggt cgatgatcga gatcccctcg
     4261 tcatcgactg cgatgatgaa ggtgtcgcgg aactgtccga gcgggatgag cggccggagt
     4321 ccccagggcg cgtccgggga aggggcggcg gcgccactcg ggctcgcgac cggcacctgc
     4381 ggcgcctgcg gcaggccggg gaacgggccc tcgtcggcgc gcgcgggcgg cacccagcgg
     4441 ctcgggaaca cgccggcaat caagcccgga aaggcctggg cggagggcgc gggctcgaag
     4501 gcgggcggcg gctgcagctg cagttcgggg gcggaggttc tgcccagcgc cgctgagatc
     4561 gcacgcttga cgacctcgtg cacgagagac tggttgcgaa agcgcacctc ggccttggtc
     4621 gggtgaacgt tgacgtccac ctcctcgggc ggcatctcga ggaacaggtg cacctcgggg
     4681 ctgcgctcct tcaccgtcgc cacgctgtag gcgtccacga tcgcgtgcgc gatggtccgg
     4741 tccttgacga tgcgccggtt cacgaagacg ttctgggggc cgcgcttggg gccctggtcg
     4801 gtgagcgacg ccaccaggcc aacgaccgag atcccggcag cctccttggc gacctcgacc
     4861 aggtcggggc gatcgccgta gagctggaag aagcggtcgc gccgcgagcg gacgggcggg
     4921 cactggaaga gcatgcggcc gccgctcgtc aacgtgaacc cgacctccgg ataggccagg
     4981 gcgagctgtg tcgccgtgcg ggaaacctgc gccgtctcgg cgccgtcggc cttgaggaac
     5041 ttgcggcggg ccgggaggtt gtagaaaagg tcggcgacct ccaccgaggt gccggggggg
     5101 gccgccacct cgcgcaccga cgcgagcgtc cccgcgttga cgcggatttc cgtgccggca
     5161 atcgcatcgg ccgccctggt ccgcaagacg aagtgcgaga ccgaggcgat ggccggcagc
     5221 gcctcgccgc ggaaaccgtg cgtgaggatc gccgccaggt cttcggcgct ggcgatcttg
     5281 ctggtggcgt ggcgcgcgag ggccagctcg gcgtcctcgg gcgacatccc ctcaccgtcg
     5341 tcctccaccc ggatcagccg cttgccgccc tgttcgacgg tgatcgcgag ccggctggcc
     5401 ccggcatcaa tcgcgttctc gaccagctct ttcaccaccg aggagggacg ctcgaccacc
     5461 tcgccggcgg cgatctggtt ggcgagctcg gcggagagac ggtgaatcct gcccatggaa
     5521 tgccgctgca gagacagagg gaaaagaaaa cgggcacggg cgagacgtct cgcccgtgcc
     5581 cgcaatcata cccgcacagg cgtgacgcct gtgccactgg gacccggttg cttcagccta
     5641 gtcccccacc ttcacggcca tcgccgcctg cgccgccgcg aggcgggcga cgggcacgcg
     5701 gaagggcgag cagctgacgt agttgaggcc gaccttctgg aagaagtgga tcgaggccgg
     5761 gtcgcccccg tgctcgccgc agacgccgag cttgaggtcg gacctcgtcg cgcgccccgc
     5821 ggtcgcgccc atcgagacca gcaggccgac gccggtcgtg tcgatgctgg cgaacgggtc
     5881 gccgtcgagg atccgcttct cctggtagat cttcaggaac ttcccggcgt cgtcgcgcga
     5941 gaagccgaag gtcatctgcg tcaggtcgtt ggtgccgaac gagaagaact gcgcctcgcg
     6001 cgcgatgtcg ccggccgtca ccgcgccgcg cggcacctcg atcatggtcc cgacgaggta
     6061 cttgatcttg acgccctgct ccttcatcac gtcctgcgcc acgcggtcga ccaccgcctt
     6121 ctggtcctgg aactccttca cgtggccgac gagcgggatc atgatctcgg gaacgacctt
     6181 cagcccttcc ttgttgagct ggcaggccgc ctcgatgatc gcgcgcgcct gcatctcggt
     6241 gatctcgggg tacgcgatcc cgaggcggca gccgcggtgg ccgagcatcg ggttgaactc
     6301 gtgcagttcc tcgacccgcg ccagcagggc gcgcttctcc tcgagctccc gggcgttgcc
     6361 gttcttcgcc tcgaggaccg cgatgtcgac catgagctcc tcgcgcttgg ggaggaactc
     6421 gtgcagcggc gggtcgatcg tcctgacggt gaccggcgtc ccgttcatcg ccttgaagac
     6481 gccgtagaag tcgccgcgct gcagcggcag cagctcggcc agcgcggcgc gccggtcggc
     6541 ctcgttgtcg gcaaggatca tgcggcggac gatcggcagc cggtcccctt cgaagaacat
     6601 gtgctcggtc cggcaaagcc cgatgccgcg ggcgccgaag gcgtaggcga tgttcgcctg
     6661 gtcgggcagg tcggcgttgg cgcggatccc catcgtgcgc ttcttgtcgg cccagccgag
     6721 caggcgctcg aagcgctggt agatctccga ctcctccggc ttcatcgtgc cgttgatgac
     6781 ctgcaaaatc tcgctcggct tggtcgccac cttctcgacc ttcacctcgc cgctgaggcc
     6841 gtcgaacgcg atgtagtcac cctcgcgcag cgtccggccg ccgatcgcga gcttcttgcc
     6901 ctcctcgtcc acgcgcagct cgtgggcgcc gacgaccgcc ggcttgccca tctggcgacc
     6961 gacgaccgcc gcgtgcgagg tcatgccgcc ggtggcagtc aggacgccct gcgacacgaa
     7021 catgccgtgg atgtcgtcgg gcacggtctc cttgcggacg agcaccacct tcttcccctg
     7081 ctcggtccac ttcaccgcct cgtcggccgt gaagacgatc tgccccgacg cggcgcccgg
     7141 cgaggcggga agccccttgg tcgacaccgg gatgcccttc cactccttgg ggtcgaagac
     7201 cggggcgagc agctgcgaga gcgacgacgg gtcgaccagc agcagcgcct cctcgggctt
     7261 caacagcttc tcggccacca tgtcggtcgc gatcacgacc gcggcgtagc cggtgcgctt
     7321 gcccgagcgg gtctggagca tgtagagctt ctcgttctcg atcgtgaact cgaagtcctg
     7381 cacgtccttg tagtgcttct cgagcagcgt ggtgtggcgc cgcagctcgc ggtacgcctg
     7441 cggcatcact tcctcgagct gggcgatcgg catcggggtc cggatgcccg cgaccacgtc
     7501 ctcgccctgg gcgttgatca ggaactcgcc gtagaactcc ttcttgccgg tcgcggggtt
     7561 gcgggtgaag ccgacgcccg tcgccgagcg ctcgccgctg ttgccgaaca ccatcagctg
     7621 cacgttcacc gcggtgccga tgtgatcggg gatctcgtag atgcggcggt actccttggc
     7681 gcgcgggttg ttccacgagc ggaacacggc gtcgcgggcg ccgcgcaact gctccatcgg
     7741 gtcgatcggg aaggagcggc cggtcttctc ccgcacgacg cccttgtagc gcttcaccac
     7801 ctcgcgcagc gcgtcttcgt cgagatcggt gtcgaccttg gcgccgcgct cgtgcttgac
     7861 ggcgtcgaac tccttctcga actcctcctt cgggatctcg aggacgacgt tgccgaacat
     7921 ctgcatgaag cggcggtagc tgtcgtacgc gaagcggccg ttgcccgtgc gcgccttcag
     7981 cccctccacc gtctcgtcgt tcagcccgag gttgaggatc gtgtccatca tgccgggcat
     8041 cgagaacttg gcgcccgacc gcaccgacac caggagcggg ttcttcaccg agccgaactg
     8101 ctgcccggcg accttctcga gcttcgcgag gttggcctcc atctcgcggt cgacctcggg
     8161 cggaaccctg cggccccgct cgtagtagag gttgcaggcc gcggtcgtga tggtgaagcc
     8221 gggcgggacc ggcagcccgg cgttggtcat ctcggccagg cccgagccct tccccccgag
     8281 caggtccttc atcgtgcggt tgccgtcggc cttgccgccg ccgaagaagt acacgtactt
     8341 cgtcgccttc ttcgccgcct tgccctgcgt gcgaacgccc ctggcggccg gccgggccgc
     8401 ccgggcggtg gtcttcgccg tgcgcctgcc tgccgcctgc ttcttcgcct tcaccatctt
     8461 cttggccatc gcgtgtgact ccagactggg aatgaatgag ccggatcagc gattcggctc
     8521 gctacgggga acaatctccg agatgtcggc caggtcgagc atcaggtcgt gcaggcgcca
     8581 gacgagcgac aaccgctcgg tgcggagccc ctcgtcctcc accatgacga acacctcggt
     8641 gaaaaagcgg tccagcctct cccggaaccc ggcggccagg cggaacgcct cgaagtactc
     8701 gcgtcgcgac gcggcggcgc gcacggccgc gccccgcgtc tcgtattcct gcagcagccc
     8761 ccgctcggcc ggctccgtca gccgtccggt cgagggcgac ggctcgccgg gcagctcgcg
     8821 ggcgaggttc ttcacccgct tgaacgccac ggccagcgcc tcgaactcgg ggagggcgcg
     8881 cacccgctgc agcgcctcga gcctcaggcg ggcgtcgaag ggcgcgtcca ggaaactccg
     8941 ccgcgcgccg gccacggcgt tcacctcgtc gtagccgaag ccgcgctgct ggaagaggaa
     9001 gcgcaggcgc tcctcgagga agaccgcgcg atcgcgccgc tccgcctggt tctcctcggg
     9061 cagcgccggg ccgccgccgc acccctcggc gtactcggcc cgcgcccgtt cgagcatcgc
     9121 ctcgaggccg accgcggcgt cgagtcctgt cagctcgcgc aggtccacga ggatcttcat
     9181 gacgccgtgc gcctggcgcc ggagccccat cgggtcgcgc gtcccggtcg gacgctcgcc
     9241 ggccgcgaag aggccgacga tcgtgtcgag cttgtcggct agcgccagcg cggcccacgg
     9301 cagggcggcc ggcccgaggt cggccctcga cggcggggcg tcggattcca ccgaggccgg
     9361 gaggtactgg ttgtagatcg cgcgccagac cgcctcgggc agcccctcct cgcgggcgta
     9421 gatccccccc atggtgccct gcagctcggt cagttcgcgc accatgtcgg tcaccaggtc
     9481 ggccttggcc agccggccgg ccagcgcggc ttcttcggcc cgggcgccct cgccgaagac
     9541 ctcgcgcgcc acccaccc
//
LOCUS       DAOPQX010000117         9554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8083_length_9554_cov_4.210864, whole genome shotgun sequence.
ACCESSION   DAOPQX010000117 DAOPQX010000000
VERSION     DAOPQX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8083_length_9554_cov_4.210864"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1121)
                     /locus_tag="PKK95_08040"
     CDS             complement(111..1121)
                     /locus_tag="PKK95_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18201.1"
                     /translation="MQRVLKVLAVLVPMVLVGCAPPADEPSAGPATAAPASEAAGGPM
                     IDLPKQVGPWERADSPKRITAATIFDYMNGGGELYLAYRFNHLDVLEYTAADASLGTI
                     LVEIYQMGSSDDAFGLLSTDWGGEPVALDPAGHPQTPFPEALYGAGLLRLRAGNLYAR
                     VLASRESPRSREQVLALGRAIANGRQGGAPPELLSQVPVPAGWSLRSDRLVFFRSHLV
                     LNSAYFLASGDILGLGSDVEGVTTECRAGRADGRAARLIVVKYPSADRAAAALRTFRD
                     AYLPETAKAAGDEGEGTAPIEEGWAAYRRNGAMLTLALDVATRDAARTLVEARGRGRA
                     GL"
     gene            complement(1149..3173)
                     /locus_tag="PKK95_08045"
     CDS             complement(1149..3173)
                     /locus_tag="PKK95_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOC18202.1"
                     /translation="MSDLNRREFMGTVAGGVAVAAAPIAGQAQGARSAARDAAPGGLA
                     DPAFRPLPLGAIRPSGWLLRQLRIQADGLSGHLDEFWPDVANSKWFGGDAEGWERAPY
                     WLDGAIPLAWLLDDGPMKARITKHVEHIVSSQRPDGWYSPYPEDAVAKRYDMWAILLA
                     NKALVQYHELTGDARVLAAVTKSLRAMHAGLDRTPLYDWGRFRWYEGLVPTFYVYECT
                     REPWLLELARKLRAQGVDFEALYKTEDITLPTPRRGLWKWTKHVVNTGMAAKAAALSW
                     RLDRRPGDRAFSRAMIDLLDRHHGQVTGMFTGDECLAGRNPLQGTELCAVVEFMYSLE
                     QLFSVFGDPWFADRLERVAFNALPATFSPDMWAHQYDQQVNQVQCTINPEHQFTTNGP
                     ESNLYGLEPNFGCCTANMHQGWPKLAAHLWMKTPDEGIVAAAYAPSRAEFRSGNTPVT
                     VSLDTDYPFRETLTITVAPTRPARFPLVLRVPAWAEGATVRVAGAAEAPMKAGTLHRI
                     ERAWQGEVQVRVRFPMRPAVVRGYNELVAVERGPLVYSLQIGEEWTRVNADKPHRELP
                     HADFEVRPTTAWNYGLILDEQHPERSLRFEERPVGERPFSPEGAGMAASAKGRKLPSW
                     KLQRGWAAEISPADVAWTNRPPATPEPAEDITLVPYGCTNIRITEFPKVE"
     gene            complement(3228..4760)
                     /locus_tag="PKK95_08050"
     CDS             complement(3228..4760)
                     /locus_tag="PKK95_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC18203.1"
                     /translation="MSLLDLFVRTAKQHPSKLAIVDRATGARVTYKNALLRSLILSRE
                     IDEFEQGFIGVMIPNSAGAILTVVATVMSGRTPVMINYSTGAEQNCRMAQRRLGFRTI
                     ITSRALLAKLKCPEVAGMVFIEDLAADIGRVAKVRGLLRSLMPAGRVIASVSRCDPDD
                     PIVILFTSGSEADPKAVPLSHRNLLANVEGMHEVLEYGPKDTILGNLPLFHVFGLNTD
                     CWLPLINAMTIVTVPNPLEFRAVCSAVREEKVTMLVGTPVFLSGYLQKSEPGDFDTVR
                     LLVTGADKCPETLRRGFWDRHRKELLEGYGTTETSPVIAVNSPDHNRPGSVGRVLPNI
                     QVRMEHYETGDTCGVGQIGKILVKGESVIKSYYDDFEATANRVRQGWYDTGDMGYMDQ
                     DGYLWHVGRLGRFLKVGGEMVSLVQVEDVLERSLPPGVLGAAVEVPDASRGSRVVAAV
                     TESVDERAVLAAMAGSLPRIALPKAFVVVAELPRMPSGKTDYRALTASVREVLAQPAA
                     VR"
     gene            5087..6331
                     /locus_tag="PKK95_08055"
     CDS             5087..6331
                     /locus_tag="PKK95_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOC18204.1"
                     /translation="MDRPLDEGFRRARTIKRTAAWGGGAAAALAVFLLAPGWISPSIS
                     HSRIRTARVDTGPIEAVVEASGIVVPEVEEVVSSPVDARVVRILARAGAELTPGQALV
                     ELDARESALAVDRLSQNLALKANAQEKTRLELQRSLNDLDSQARLKDLQLQSYRSQLA
                     RNRALSKEGLISEEVLKQSELAEAQAVVELKKIQAERENAERMTRATLDGLGLEMRTL
                     AKEVEEARRQLTLASPKATRRGVLTWSLSEEGIAIRKGDVIARIADLSSFRVDATLSD
                     VHAKRVSGGLPVIVRVGEEALEGVVSTVLPTIRNGVMTLQIALTQKSSPLLRSNLRVD
                     AFIVTGRRAKALRIRRGPFAGGEGPADVFVVRDGRGVRTRVELGIAGLDDYEVVSGLA
                     EGDEVIVSDMKDYLHLREIRIK"
     gene            6355..7032
                     /locus_tag="PKK95_08060"
     CDS             6355..7032
                     /locus_tag="PKK95_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18205.1"
                     /translation="MTNTMIRLENVEKVYRTERIETVALSGISVEVREGEFVSVMGPS
                     GCGKSTLLNVMGLLDQPTSGAVHLDGASIDSYQDRRLARIRNRQIGFVFQTFHLIADL
                     SVLDNVEIPLLYRRTPAGERRQQAMEALERVGLGSRVHHFPSQLSGGQQQRVAIARAI
                     VGRPRILLADEPTGNLDSQMGEEIMAILQVLNQQEKTTIVMVTHDQRLACQTSRILRL
                     FDGRMVN"
     gene            7042..8286
                     /locus_tag="PKK95_08065"
     CDS             7042..8286
                     /locus_tag="PKK95_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18206.1"
                     /translation="MLLHYLKTAWKVFLRRKFFTLISLFGISFTLVVLTVAVALLDHN
                     FGPHPPEVHHDRTRAVLRATMLGEHNTSNSGAGYKLLDRYARNLPGVEIVSIASQPAA
                     AQSFLGGQKITLQVRRTDAEFWRILAFDFLEGGPFDARDVAEARFVAVINRATRDRLF
                     AGRPALGLTFEADGQRFRVVGVVGDVPTMRRMSSADAWVPLTTAKTDAYKNDLLGPFM
                     GIALLERGASARQVNAELSARLKRAALPDPKNYDRLIARFETPLENLASELFGARGEE
                     EPHLERLWAAIAIAGLLFMLLPTVNLVNLNVSRMMERASEIGVRKAFGASSRTLVGQF
                     VVENVALSLIGAVAGFVLTEAVLRAVTASGILPYARLGINFRVFFSGVAIAVFFGVLS
                     GVYPAWRMSRMNPVQALKGASR"
     gene            8283..9503
                     /locus_tag="PKK95_08070"
     CDS             8283..9503
                     /locus_tag="PKK95_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18207.1"
                     /translation="MIRHYLKLTWNRRRSNLLIAVEILLSFVVTFIVVTLAAHGADNY
                     RRPLGFEWRDAWNVIAYAQLGHPGEHGEEMPPEVLETARQVFAAIREFPEVESVGGTT
                     CPPYGFSEWTSSSDIDGWHLRYGVNLATDGLASALGLQLTRGRWFDARDDGAAWVPVV
                     VNERLAAEVARGGDPIGMSIPRKKEQDATEEPELRIVGVVGDFRQNGEFATPQNYLFR
                     RHRLDDATRRPPENLIVRLRPGTTAAFEEKLVNRLQAVAPDWSFEVTPLEQMRETRFR
                     MVLGPLIGAAVIAGFLLLMVAMGLTGVLWQTVTQRTREIGLRRAKGATSGDIRKQVVG
                     ELLVLTLIAVAAGTALALQFPLLELTGPISGRVFAVGLLASAACICALTMACAWYPSL
                     IATRVTPAEALHYE"
BASE COUNT         1437 a         3266 c         3387 g         1464 t
ORIGIN      
        1 caagggttcc ccggacgaag tcgcgacgag tgatgttgcg catgcgccct cccgtaccgt
       61 cggtcctcgc agatgttcca cagagccgaa cccgcttcct cagcggcctg ctagaggccc
      121 gctctgccgc gaccgcgtgc ctcgaccagc gttcgtgcag cgtcgcgggt cgccacgtcg
      181 agcgcgagcg tcagcatcgc gccgtttcgc cggtatgccg cccaaccctc ctcgatcggc
      241 gcggtcccct cgccctcgtc gcctgcggcc ttcgccgtct cgggaaggta ggcatcccgg
      301 aaggtgcgaa gggcggcggc cgcccggtcg gcagaggggt acttcacgac gatgagacga
      361 gcagcccggc cgtcggcgcg gcccgcccgg cactccgtgg tcacgccctc gacgtcggag
      421 ccgaggccga ggatgtcgcc cgaggccagg aagtaggcgg agttcagcac caggtgcgag
      481 cggaagaaca cgaggcgatc ggaccgcagc gaccagcccg cgggaaccgg cacctggctc
      541 aacagctctg gcggcgcgcc accctgacgc ccgtttgcga tggcccggcc cagggcgagc
      601 acctgctcgc gtgacctcgg tgactcgcga gaagcgagga cgcgggcgta gaggtttccc
      661 gcccggaggc gaagcaggcc ggccccgtag agcgcctccg gaaagggggt ctgagggtgg
      721 ccggccgggt cgagcgccac cggttcgcct ccccagtcgg tggacagcag cccgaaggcg
      781 tcgtcggagg accccatctg gtagatctcg accaggatgg tgccgagcga cgcgtcggca
      841 gctgtgtatt cgagaacgtc caggtggttg aagcggtacg cgaggtacag ctcgccgccg
      901 ccgttcatgt agtcgaagat cgtcgcggcg gtgattcgtt tcgggctgtc ggccctctcc
      961 cacggcccca cctgcttcgg cagatcgatc atcggccctc cagcggcctc gctcgccggt
     1021 gccgccgtcg ccgggccggc ggacggttcg tcagcgggcg gcgcgcaccc gaccagcacc
     1081 atcggcacca gcaccgccag caccttcagc accctctgca cctcccgcat ctccgtacga
     1141 cttcccggct actcgacctt cggaaattcc gtgatccgga tgttcgtgca gccgtagggg
     1201 acgagcgtga tgtcctcggc cggctcgggc gtcgccgggg gccggttggt ccacgccacg
     1261 tccgccggcg agatctccgc cgcccagccg cgctgcagct tccagctcgg cagcttgcgc
     1321 cccttcgccg acgccgccat gccggcgcct tcgggcgaga acggccgctc gccgaccggg
     1381 cgctcctcga agcgcaggct gcgctccggg tgctgctcgt ccaggatcag gccgtagttc
     1441 caggcggtcg tgggccgcac ctcgaagtcg gcgtgcggca gctcgcggtg cggcttgtcg
     1501 gcgttgacgc gcgtccactc ctcgccgatc tgcagcgagt agacgagggg cccgcgctcg
     1561 acggccacca gctcgttgta gccgcgcacg acggccgggc gcatcgggaa gcggacgcgc
     1621 acctgcacct cgccctgcca ggcgcgctcg atgcggtgca gcgtcccggc cttcatgggc
     1681 gcctcggcgg cgccggccac acgcacggtc gctccctccg cccacgcggg aactcgcagc
     1741 acgagcggga agcgcgccgg gcgcgtcggg gccaccgtga tcgtcagcgt ttcccggaac
     1801 gggtagtcgg tgtcgagcga gacggtgacc ggcgtgttgc ccgagcggaa ctcggcccgg
     1861 ctcggcgcat aggcggccgc gacgatcccc tcgtccggcg tcttcatcca aaggtgcgcg
     1921 gccagcttgg gccagccctg gtgcatgttg gccgtgcagc agccgaagtt cggctcgagc
     1981 ccgtacaggt tcgactcggg gccgttggtg gtgaactggt gctcggggtt gatcgtgcac
     2041 tgcacctggt tgacctgctg gtcgtactgg tgcgcccaca tgtcggggct gaaggtggcg
     2101 ggcagcgcgt tgaacgccac gcgctcgagc cggtcggcga accaggggtc gccgaagacc
     2161 gagaacagct gctcgagcga gtacatgaac tcgaccaccg cgcacagctc ggtcccctgg
     2221 agggggttgc ggccggcgag gcactcgtcg ccggtgaaca tgccggtgac ctggccgtgg
     2281 tgccggtcga gcaggtcgat catcgcccgc gagaacgcgc ggtcgccggg gcgccggtcg
     2341 agccgccacg aaagggccgc ggcctttgcc gccatccccg tgttgaccac gtgcttggtc
     2401 cacttccaca ggccgcgccg gggcgtcggc agcgtgatgt cctcggtctt gtagagggcc
     2461 tcgaagtcca ccccctgcgc gcgcagcttc cgcgccagct cgaggagcca cggttcacgg
     2521 gtgcactcgt acacgtagaa cgtagggacg agaccttcgt accaccggaa gcggccccag
     2581 tcgtagagcg gcgtccggtc gagacccgcg tgcatggcgc ggaggctctt ggtcaccgcg
     2641 gcgagcacgc gcgcgtcgcc ggtcagctcg tggtactgca ccagcgcctt gttggcgagg
     2701 aggatcgccc acatgtcgta gcgcttcgcg accgcgtcct cggggtaggg gctgtaccac
     2761 ccgtcggggc gctggctcga gacgatgtgc tcgacgtgct tcgtgatgcg cgccttcatc
     2821 ggcccgtcgt ccagcagcca ggcgagcggg atggcgccgt caagccaata gggcgcccgc
     2881 tcccagccct cggcgtcacc gccgaaccac ttgctgttcg ccacgtcggg ccagaactcg
     2941 tcgaggtggc cgctgaggcc gtcggcctgg atccgcagct ggcgcagcag ccagccggac
     3001 ggccggattg caccgagcgg gagcgggcga aacgcggggt cggcgaggcc gcccggggcg
     3061 gcgtcgcggg ccgccgacct cgcgccctgc gcctggccgg ctatcggcgc cgcggccacg
     3121 gccacaccgc ccgcgacggt tcccatgaac tccctccggt tcaggtcaga catggtctct
     3181 cccactccca atcgacacgt cgccgatccc tgatccctgt tccctactca ccgtactgcg
     3241 gccggctgcg cgagcacctc gcggacgctg gcggtgaggg cgcggtagtc ggtcttgccg
     3301 ctcggcatcc gcggcagctc ggcgaccacc acgaacgcct tcggcagggc gatgcggggg
     3361 agcgagccgg ccatggccgc gagcaccgcg cgctcgtcca ccgactcggt gacagcggcc
     3421 accacgcgcg agccgcggct ggcgtcgggc acctcgacgg cggcgccgag gacgcccggc
     3481 ggcagcgacc gttcgagcac gtcctcgacc tggacgagcg acaccatctc gccgccgacc
     3541 ttgaggaagc gcccgagccg gccgacgtgc cacaggtagc cgtcctgatc catgtacccc
     3601 atgtcgccgg tgtcgtacca gccctggcgc acgcggttgg cggtcgcctc gaagtcgtcg
     3661 tagtacgact tgatgacgct ctcgcccttc acgaggatct tgccgatctg gccgacgccg
     3721 cacgtgtcgc ccgtctcgta gtgctccatc ctgacctgga tgttgggcag cacgcggccg
     3781 acgcttcccg ggcggttgtg gtcgggggag ttgaccgcga tgaccgggct ggtctcggtg
     3841 gtgccgtacc cctcgagaag ctccttgcgg tgccggtccc agaagccgcg ccgcagcgtc
     3901 tcggggcact tgtcggcgcc ggtgacgagc agccgcacgg tgtcgaagtc gcccggctcc
     3961 gacttctgca ggtatcccga caggaacacc ggcgtcccca ccagcatcgt caccttctcc
     4021 tcgcggaccg ccgagcagac ggcgcggaac tcgagggggt tggggacggt gacgatcgtc
     4081 atcgcgttga tgagcgggag ccagcagtcg gtgttgaggc cgaagacgtg gaagagcggc
     4141 aggttgccga gaatggtgtc cttcgggccg tactcgagga cctcgtgcat gccctcgacg
     4201 ttggcgagca ggttgcggtg gctgagcggg acggccttcg ggtcggcctc gctgccgctg
     4261 gtgaagagga tcacgatcgg gtcgtcagga tcgcagcggg agaccgaggc gatgacacgt
     4321 cccgccggca tcagcgaccg cagcaggccc cggaccttgg cgacgcggcc gatgtcggca
     4381 gccaggtcct cgatgaagac catgccggcc acctcggggc acttgagctt cgccagcagg
     4441 gcgcgcgacg tgatgatggt gcggaacccg aggcggcgct gggccatccg gcagttctgc
     4501 tcggcgccgg tcgagtagtt gatcatcacg ggggtccgtc cgctcatcac ggttgcgacc
     4561 accgtcagga tggccccggc cgagttcggg atcatcacgc caatgaaccc ctgctcgaac
     4621 tcgtcgatct cacgcgacag gatgagcgat cggagcagtg cgttcttgta ggtcacccgg
     4681 gcgcctgtgg cgcggtcgac gatggccagc ttggaggggt gctgcttggc ggtccggacg
     4741 aacaggtcca gaagggacac ggtggcctcc aggggcgggg tgaacggggg ccggcaagag
     4801 gggattatcc tactccagca gacgggatca gggatcaggg atcggggatc agggatcggg
     4861 gatcagggac ggggaccggc cgacggcctc actcccgtga cagtgccagg tagagggcca
     4921 gcggggcgag caccagggcg gcggccgcat cgcggctcac ttgtccggta ccggacaggg
     4981 ccgtacgcta ccggacgacc gcgaggcgcc aaaccgcgca tttccgccca aatccaagcg
     5041 ccgtcctggt acctcgcttg cggccccccg gggccggagg cgagaggtgg accgacccct
     5101 tgacgaggga ttccgtcgcg cccggacgat caagcgcacc gccgcgtggg gcgggggggc
     5161 cgccgcggcc ctggcggtgt tcctgttggc gcccggctgg atcagcccct cgatctccca
     5221 ctcgcgaatc cggaccgccc gcgtggacac cggcccgatc gaggcggtcg tcgaagcctc
     5281 cggcatcgtc gtccccgagg tcgaggaagt ggtgtcgagc cccgtcgatg cccgggtggt
     5341 tcgcatcctg gcgcgggcgg gggccgagct gactccgggc caggcgctgg tggagctcga
     5401 tgcccgcgag tcggcactcg ccgtggaccg cctgtcgcag aacctcgcgc tcaaggccaa
     5461 cgcccaggag aagacgcgcc tcgagctgca gcgaagcctc aacgatctcg acagccaggc
     5521 gcgcctcaag gacctgcagc ttcagtccta ccgctcgcag ctcgcgcgca accgcgcgct
     5581 ctcgaaggaa ggcttgatct ccgaggaggt attgaagcag tccgagctgg ccgaggccca
     5641 ggccgtcgtc gagctgaaga agatccaggc cgagcgcgag aacgccgagc ggatgacgag
     5701 ggcgacgctc gacggcctcg gcctcgagat gcgcacgctg gccaaggagg tcgaagaggc
     5761 gcggcgccag ctgacgctgg cctcgccgaa ggccacccgc cgcggcgtgc tcacctggtc
     5821 gctctccgag gaggggatcg ccatccgcaa gggcgacgtg atcgcccgca tcgcggatct
     5881 gtcgtcgttc cgggtggacg ccaccctgtc ggacgtccac gcgaagcggg tgagcggcgg
     5941 cctgccggtg atcgtgcggg tgggcgagga agcgctcgag ggggtcgtct cgaccgtcct
     6001 gccgacgatc cgcaacggcg tcatgacgct gcagatcgcg ctgacgcaga agtccagccc
     6061 gctgctgcga tcgaacctcc gcgtggatgc cttcatcgtg acgggacggc gggcgaaggc
     6121 ccttcgcatc cgccgcggcc ccttcgcggg gggcgagggg cccgccgacg tgttcgtggt
     6181 gcgggacggg cgcggcgtga ggacgcgcgt cgagctgggg atcgccgggc tcgacgacta
     6241 cgaggtcgtc agcggcctcg ccgagggcga cgaggtgatc gtctccgaca tgaaggacta
     6301 cctgcacctg agggagatca ggatcaagta gcggtgcccg acgaaagtgg agccatgacc
     6361 aacaccatga tccggctgga gaacgtcgag aaggtctacc gcaccgagcg gatcgagacc
     6421 gtcgcgctga gcggcatcag cgtggaggtg cgcgaggggg agttcgtgtc ggtgatgggc
     6481 ccgtcgggct gcggcaagag caccttgctg aacgtgatgg ggctgctcga ccagccgact
     6541 tccggcgcgg tccacctcga cggcgcctcc atcgactcgt accaggaccg gaggctcgcc
     6601 aggatccgga accggcagat cggcttcgtc ttccagacct tccacctgat cgccgacctg
     6661 agcgtcctcg acaacgtgga aatcccgctc ctctatcgcc ggacgccagc cggcgaacgg
     6721 cggcagcagg cgatggaggc gctcgagcgg gtcggcctgg gttcgcgcgt gcaccacttc
     6781 ccgtcgcagc tctcgggtgg gcagcagcag agggtcgcca ttgcccgcgc catcgtgggc
     6841 aggccgcgga tcctgttggc cgacgagccg acagggaacc tcgacagcca gatgggtgag
     6901 gagatcatgg ccatcctgca ggtcctcaac cagcaggaga agacgacgat cgtgatggtg
     6961 acgcacgacc agcggctcgc gtgccagaca agccgcatcc tccgcctgtt cgacgggcgg
     7021 atggtgaact aggggcgtcc catgctgctg cactacctga agaccgcgtg gaaggtgttc
     7081 ctgcggcgga agttcttcac gctgatcagc ctgttcggca tcagcttcac gctggtcgtc
     7141 ctcaccgtcg cggttgcgct gctcgaccac aacttcgggc cgcatcctcc ggaggtgcac
     7201 cacgaccgca cccgtgcggt gctgcgggcg accatgctgg gcgagcacaa cacctcgaac
     7261 tcgggcgcgg gatacaagct gctggaccgg tacgccagga acctgccagg ggtcgaaatc
     7321 gtctcgatcg cgtcccagcc cgccgccgcc cagtcgttcc tgggcgggca gaagatcacg
     7381 ctgcaggtgc gccgcaccga cgccgagttc tggcgcattc tcgccttcga cttcctcgag
     7441 ggagggccat tcgacgcgcg tgacgtggcc gaggcccgct tcgtggccgt catcaaccgt
     7501 gccacgcgcg atcggctctt cgccggccgg cccgcgctcg gcctgacctt cgaggccgac
     7561 ggccagcgct tccgcgtcgt gggcgtcgtc ggcgacgtgc caaccatgcg gcggatgtcc
     7621 tccgccgacg cctgggtgcc gctgaccacg gcgaagaccg acgcgtacaa gaacgacctg
     7681 ctcgggccgt tcatggggat cgcgctgctc gagcgggggg cttcggcccg ccaggtcaac
     7741 gcagaactct ccgctcgact gaagcgcgcc gcgcttcccg atcccaagaa ctacgaccgg
     7801 ctcatcgccc gcttcgagac gccgctcgaa aacctcgcca gcgagctttt cggggcccgg
     7861 ggcgaggagg agccgcacct ggagcggctc tgggctgcca tcgcgattgc aggcctcttg
     7921 ttcatgctgc tgccgaccgt gaacctcgtg aacctgaacg tcagccggat gatggagcgg
     7981 gcatcggaga tcggcgtccg aaaggcgttc ggggcctcgt cgcgcacgct ggtcggccag
     8041 ttcgtcgtcg agaacgtcgc cctctcgctg atcggggccg tggcgggatt cgtcctcacc
     8101 gaggccgtgc tccgcgcggt gacggcgagc ggtatccttc cctatgcgag gctcggcatc
     8161 aatttccggg tcttcttctc cggcgtcgct atcgcggttt tcttcggggt gctctcgggc
     8221 gtgtacccgg cgtggcgaat gtcgcggatg aacccggtgc aggcgctgaa gggagcgtcg
     8281 cgatgatccg ccactacctg aagctgacgt ggaaccggcg gcgctcgaac ctgctgatcg
     8341 cggtcgagat cctcctctcg ttcgtggtga ccttcatcgt ggtgacgctc gccgcccacg
     8401 gcgccgacaa ttaccgccgc ccgctcgggt tcgagtggcg ggacgcgtgg aacgtgatcg
     8461 cgtacgcgca acttgggcac cccggcgagc acggcgagga gatgccgccc gaggtgctcg
     8521 agacggcacg acaggtcttc gccgcgatcc gcgagtttcc cgaagtcgag tctgttgggg
     8581 ggacgacgtg ccccccttac ggcttctcgg agtggacctc ctcgtcagac atcgacggat
     8641 ggcacctgcg ctatggggtg aacctggcaa ctgacggcct ggccagcgcc ctcggcctcc
     8701 agctcacccg cgggcgctgg ttcgatgcca gggacgacgg ggccgcctgg gtgcccgtgg
     8761 tcgtcaacga acgcctcgcc gcggaggtgg ccaggggggg cgacccgatc ggcatgagca
     8821 tcccgcggaa gaaggagcag gacgcaaccg aggagccgga acttcgcatc gtgggcgtgg
     8881 tcggcgactt ccgccagaac ggcgagttcg ccacgccgca gaactacctc ttcaggcggc
     8941 accgcctcga cgatgccacg cgcaggccgc ccgagaacct gatcgtccgc ctgaggccgg
     9001 ggaccaccgc ggccttcgag gaaaagctgg tgaatcggct gcaggccgtc gcgccggact
     9061 ggtcgttcga ggtgacaccg ctcgagcaga tgcgcgagac acgtttccgg atggtgctcg
     9121 gcccgctcat cggggccgcg gtaattgcag gcttcctgct gctgatggtc gcgatggggc
     9181 tgacaggcgt gctctggcag acggtgacgc agcggacgcg cgagatcggg ctgcgccgcg
     9241 ccaagggggc tacctcgggc gatatccgga agcaagtggt gggtgagctg ctggtcctga
     9301 ccctgattgc cgtcgcggcg ggaaccgcgc tcgcgctcca gttcccgctg ctcgagctga
     9361 ccggaccgat cagcggccgc gtgttcgccg tgggtctcct cgcgtcggct gcctgcatct
     9421 gcgctctcac gatggcctgc gcttggtacc cgagcctgat cgccacccgc gtgacaccgg
     9481 ccgaggcgct gcactacgaa taggggctgg gagctagggg ctgggggcga gcctagcccg
     9541 cgatgatctt gaac
//
LOCUS       DAOPQX010000118         9443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8217_length_9443_cov_4.362804, whole genome shotgun sequence.
ACCESSION   DAOPQX010000118 DAOPQX010000000
VERSION     DAOPQX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9443
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8217_length_9443_cov_4.362804"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKK95_08075"
     CDS             <1..380
                     /locus_tag="PKK95_08075"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18208.1"
                     /translation="DALRAHFPPEFLNRIDEVIVFHPLGRQHLEQIVDIQLRGLLQRL
                     QARKIDVELTDRARQHIVAEGYDPVYGARPLKRTLQRLLLDPLAMQVLAGAFREGDTV
                     LVDGANGGLTFEKAAAPTSGVAS"
     gene            426..2324
                     /gene="ftsH"
                     /locus_tag="PKK95_08080"
     CDS             426..2324
                     /gene="ftsH"
                     /locus_tag="PKK95_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOC18209.1"
                     /translation="MPPTQKPRLTPPRGGRPGERRPAGPRPVSSVWYVLGFLLLLAVV
                     QAWLFIPAGRTIPYSEFKQLLAAGRVAEVTVSQDTIRGRLKDGQTDKERAFTTTRVED
                     PKLVEELAQQKVQYTGEVTSRWLPELLGWMIPLLLIIGLSGFFFRRMSSGEGGVMSFA
                     RSRGKIYAEDDVKVSFGDVAGVDEAEDELREIVEFLKTPKKYTQLGGRIPKGVLLVGP
                     PGTGKTLLARAVAGEARVPFFSLSGSEFVEMFVGVGAARVRDLFQQAEAKAPCIVFID
                     ELDALGKVRVQSPIGSHEEREQTLNQLLAEMDGFDSRKGVIIMGATNRPEVLDPALLR
                     PGRFDRQVLVDKPDVRGREAILRIHAKGVKTAPEVDLQVVAARTAGFAGADLANLTNE
                     AALLAARKNKPHVEMRDFDEAIDRIVAGLEKKRVMSDRERQIIAYHESGHALVATLVP
                     GLDPVHKISIVQRGFGALGYTMQLPLEDRYLMTRSDLYNQLAVLLAGRSAEEIALGEI
                     STGAQNDLQRASDIARAMVTEFGMSDALGSVSFDTRGAPRFLDVPVGGERGAYGEETA
                     RQIDAEVRRIVTEAHQRARALVASRRETLEQIARRLLEKEVIEGEELRAIVNGPPPEE
                     INTEEGRM"
     gene            complement(2396..3751)
                     /gene="rho"
                     /locus_tag="PKK95_08085"
     CDS             complement(2396..3751)
                     /gene="rho"
                     /locus_tag="PKK95_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOC18210.1"
                     /translation="MQARGSNGQTVPAEPPAAEPQDPAKAQPRRQGDGLNIGELKDMS
                     IQKLTQIAKDLNVAGATGMRKQELIFQILKAQTEQSGYIFSEGVLEVLPDGFGFLRAP
                     DYNYLPGPDDIYVSPSQIRKFDLQTGDTVSGQIRPPKEGERYFALIKVEAVNFEPPEQ
                     ARDKLFFENLTPLYPQEMCKLETQPDNLSGRVMDLMTPIGKGQRGLIVAPPRTGKTML
                     LQSIAQSIAVNHPEVYLIVLLIDERPEEVTDMQRSVDGEVISSTFDEPAQRHVQVAEM
                     VIEKAKRLVEHKKDVLILLDSITRLARAYNTVIPPSGKVLSGGLDSNALQKPKRFFGA
                     ARNIEEGGSLTIIATALVDTGSRMDDVIFEEFKGTGNMEIHLDRKLVDKRVFPAIDIQ
                     KSGTRKEELLLAREDLNRVWVLRKVLNPLSPVEAMELLLDKMSKTKSNAEFLSSMQKM
                     G"
     gene            complement(4015..4386)
                     /locus_tag="PKK95_08090"
     CDS             complement(4015..4386)
                     /locus_tag="PKK95_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOC18211.1"
                     /translation="MAKVVAVIGASSNRRRYGNKAVRAFSHKGFTVVPVTPSEAEVEG
                     LKTYPTVLDVPGAIDMATFYVRPAIGLQVIDQVARKGIGEVWLNPGAGSEELERKAAS
                     LGIRTVAACSILGIGEDPSRY"
     gene            4472..6121
                     /locus_tag="PKK95_08095"
     CDS             4472..6121
                     /locus_tag="PKK95_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC18212.1"
                     /translation="MRAAAILLVLLSGLTFGLGLGTPAITDSDEAYYAEASREMVERG
                     DYLTPRFNYRERFQKPVLYYWAAAGFYQLFGIREWTARAAAALSGLGLVLLAFAAGRR
                     WFDAATGLLAGAVLATSFGAVFMARMALPDLPLAFFVNATVYTALVALFERPARVARW
                     WAACGAVAGLGFLTKGPVAILLPALVIAPLFVFERGWRRLRARHVALALAAFALTALP
                     WYAAMTAEHGTRYLYGFFLGDNLERFATTAYNPHRSPFFYVPILLGGLLPWSPFLFLG
                     IRPLLAAWRARRLSRRSVRLLCWSVLPLLFFSVSVGKQPRYILPMLPPLTLAIAWWIR
                     TATLGRQGAAGEQPALARWLGFASGGLVFTLAVLLWRAQPLLQNVSPARHLAGVAALA
                     GTGVAVMAIAASRRWRLTPAAVAVAGAAIVLALNQAVLSERGAYPVERMARAVTAERG
                     AGEPVGTYRVFVRNLIFYTGVEQEDLYSEARLHDFLDSPDRVLCVIREHDLLALEQSR
                     ARPYRRLRTVTYFDPATAKLRALLWPDPERELRTVVLISNK"
     gene            complement(6152..7534)
                     /locus_tag="PKK95_08100"
     CDS             complement(6152..7534)
                     /locus_tag="PKK95_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOC18213.1"
                     /translation="MLPPAFVEEMRQIVGRDRVRDDPAALDAYGQDALARVHPPDLVV
                     IPGTTLEVSAIAEACSTRAIPLTVRGGGTGYTGGSVPLRGGVVVSMERFNRILEIDQD
                     NLLAVVQPNVITGALQAAVERVGLFYPPDPASLEKSVIGGNIAECAGGPRAFKYGTTR
                     RYVLGLEAVLPGGEVIRTGSKAVKNVVGYDLTQLLVGSEGTLAIITQAVLRLVPLPPS
                     RATLRATFPSAGAAVEAVTGLIRSRVVPATLELVDAASLAAVARYLGEELAPAGTGAL
                     LLVEVDGLAEAVAEEARRVAAACGAAGATEVLRASTSEERDRLWRVRRELSPALKTIA
                     PTKLNHDVVVPRGRIADLFELLQELRDGFDLTIACFGHAGDGNIHVNFMVHADDPAEM
                     ARARRAERLLFEGVVAREGSISGEHGVGFTKAPYLGLELSPDTIALMKRVKHAFDPKG
                     ILNPGKIWTE"
     gene            complement(7579..8811)
                     /locus_tag="PKK95_08105"
     CDS             complement(7579..8811)
                     /locus_tag="PKK95_08105"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOC18214.1"
                     /translation="MGVSQFARSIAESPTLALNDQARLLREQGEPVVHLGVGEPQNRA
                     PQAAIEKGTAALHGGKIKYSPTTGLASFKQAIIRYTEETYGRTVAPRNVIVTSGAKQA
                     IFNALFAILDAGDEVILLAPYWVSYPEMVKMCRAVPVVVSSDPGSLEPDVARVEAAVT
                     SRTRAIVVNSPNNPSGMVYSPDFVGSIVRMCEERGIHAIMDDIYHKLVFDGVTAPSCY
                     DYTRLETDGSHLIVVNGIAKTYGMTGFRIGWAIASREIVGAMSKIQSQTTSCVSSLCQ
                     VAAEGALLGPQDEVETLRRAMEHNRDVIVDELGKLPGVRVVKPQGTFYCLPDFSHYLK
                     PGIAADSFELSMFLLNKARVVTVPGRDFGLEGHLRLSYAGSTVDVVEGMRRIRWALDA
                     GEPREIEIGGRTLVREWA"
     gene            complement(8827..>9443)
                     /locus_tag="PKK95_08110"
     CDS             complement(8827..>9443)
                     /locus_tag="PKK95_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18215.1"
                     /translation="GHYSKERIMFVFVLNCGSSSFKYQLLDMSNEHRVAVGLVERIGM
                     KDSILAYEPANGEKVKEVVDIANHEIAIKMVLDKLVAPKTGVIKSLADIAAVGHRVVH
                     GAEKFSGSVLICDRVMEALRENIPLAPLHNPANITGIEAMQKAMPGVPNVGVFDTAFH
                     STMPAEAYTYISSRLIREVFALGGPIAGLVPEAVEARMHARRAR"
BASE COUNT         1376 a         3254 c         3278 g         1535 t
ORIGIN      
        1 tggacgcgct gcgcgcccac ttcccgccgg agttcctcaa ccggatcgac gaggtgatcg
       61 tcttccaccc gctcgggcgc cagcacctgg agcagatcgt cgacatccag ctgcgcggcc
      121 tgctgcagcg cctccaggcc cggaagatcg acgtcgagct gaccgaccgg gcacgccagc
      181 acatcgtcgc cgaggggtac gacccggtgt atggcgcgcg gccgctgaag cggaccctgc
      241 agcggctgct gctcgacccc ctggccatgc aggtactcgc gggcgcgttc cgcgagggcg
      301 acacggtgct cgtggacgga gccaacggcg gcctgacctt cgagaaggcc gccgcgccga
      361 cgagcggggt cgcatcgtga accgtgcggc cggtgaatcg atcgtctgac ccccgaggag
      421 ccagcgtgcc acccactcag aagccccgcc tcaccccacc gcgcgggggg cgtcccggcg
      481 aacgccggcc cgcggggccg cgcccggtct cgtcggtctg gtacgtcctc gggttcctgc
      541 tgctgctggc cgtcgtgcag gcctggctgt tcatcccggc cggccgcacc atcccgtaca
      601 gcgagttcaa gcagctgctc gccgccggcc gggtggccga ggtcaccgtc agccaggaca
      661 ccattcgcgg gcgcctgaag gacggccaga ccgacaagga acgggccttt accacgacgc
      721 gggtcgaaga tccgaagctc gtcgaggagc tggcgcagca gaaggtgcag tacaccggcg
      781 aggtgacgtc acggtggctg cccgagctgc tggggtggat gatcccgctg ctgctcatca
      841 tcggcttgtc cggcttcttc ttccggcgca tgagcagcgg cgaggggggc gtcatgtcgt
      901 tcgcccgcag ccgcgggaag atctacgccg aggacgacgt gaaggtgagc ttcggcgacg
      961 tcgcgggggt ggacgaggcc gaggacgagc tgcgcgagat cgtcgagttc ctcaagacgc
     1021 cgaagaagta cacccagctc ggcgggcgga tcccgaaggg cgtgctcctg gtcgggccgc
     1081 ccggcaccgg caagacgctg ctggcgcggg cggtcgcggg cgaggcgcgg gttcccttct
     1141 tcagcctgag cggctcggag ttcgtcgaga tgttcgtggg cgtcggggcg gcgagggtgc
     1201 gcgacctgtt tcagcaggcc gaggcgaagg cgccctgcat cgtcttcatc gacgagctgg
     1261 acgcgctcgg aaaggtgcgg gtgcagagcc cgatcggcag ccacgaggag cgcgagcaga
     1321 cgctcaacca gctgctggcc gagatggacg gcttcgactc gcgcaagggc gtgatcatca
     1381 tgggggccac caaccggccc gaggtgctcg acccggccct gctgcggccc gggcggttcg
     1441 accggcaggt gctggtggac aagcccgacg tgcgggggcg cgaggcgatc ctccgcatcc
     1501 acgcgaaggg agtgaagacg gcgcccgagg tcgacctgca ggtggtggcg gcgaggacgg
     1561 cggggttcgc cggggccgac ctggccaacc tgaccaacga ggcggcgctg ctggcggcgc
     1621 gcaagaacaa gccgcacgtc gagatgcgcg acttcgacga ggcgatcgac cggatcgtcg
     1681 cggggctcga gaagaagcgg gtgatgagcg accgcgagcg gcagatcatc gcctaccacg
     1741 agtcggggca cgcgctggtg gcgacgctgg tccccgggct cgacccggtg cacaagatct
     1801 cgatcgtgca gcgcgggttc ggggccctcg gctacacgat gcagctgccg ctcgaagacc
     1861 ggtacctgat gacgcgctcg gatctctaca accagctcgc ggtgctgctg gcgggccggt
     1921 cggccgagga gatcgcgctc ggcgagatct ccaccggggc gcagaacgac ctgcagcggg
     1981 ccagcgacat cgcgcgggcg atggtgaccg agttcgggat gagcgacgcc ctcggctcgg
     2041 tgagcttcga cacgcggggc gccccccgct tcctcgacgt gccggtcggc ggcgagcggg
     2101 gcgcctacgg cgaggagacc gcgcggcaga tcgacgccga ggtgcggcgg atcgtcaccg
     2161 aggcgcacca gcgcgcccgc gcgctggtcg cctcgcgccg ggaaacgctc gagcagatcg
     2221 cccggcggct gctggagaag gaagtgatcg agggcgagga gctgcgggcg atcgtgaacg
     2281 gaccgccccc ggaagagatc aacactgagg aaggcagaat gtaaggaagg ccggaacgcg
     2341 tgacgcgcgc gctccggcct tctccattct acatttcggg cgtcggtccg gtcccctacc
     2401 ccatcttctg catcgaggag aggaactcgg cgttcgactt ggtcttcgac atcttgtcga
     2461 gcagcaactc catcgcctcg accggggaga gggggttgag gaccttgcgg agcacccaca
     2521 cgcggttgag gtcctcgcgc gcgagcagca gctcctcctt ccgggtaccg ctcttctgga
     2581 tgtcgatcgc cgggaagacc cgcttgtcca ccagcttgcg atccaggtgg atctccatgt
     2641 tgccggtgcc cttgaactcc tcgaagatga cgtcgtccat ccgcgagccg gtgtccacca
     2701 gggccgtggc gatgatcgtc agcgagccgc cctcctcgat gttgcgcgcc gcgccgaaga
     2761 agcgcttcgg cttctgcagc gcgttcgagt cgaggccgcc cgagagcacc ttacccgagg
     2821 gagggatcac ggtgttgtag gcccgcgcga ggcgcgtgat cgagtcgagc aggatgagga
     2881 cgtccttctt gtgctcgacc agccgcttcg ccttctcgat gaccatctcg gccacctgga
     2941 cgtggcgctg cgccggctcg tcgaaggtcg aggagatgac ctcgccgtcc accgaccgct
     3001 gcatgtcggt gacctcctcg ggccgctcgt cgatgagcag gacgatcagg tacacctcgg
     3061 ggtggttgac cgcgatcgac tgggcgatgc tctggaggag catcgtcttg ccggtgcgcg
     3121 gcggcgcgac gatcaggccg cgctgcccct tcccgatcgg ggtcatgagg tccatgacgc
     3181 ggccggacag gttgtcgggc tgcgtctcga gcttgcacat ctcctgcggg tagagcggcg
     3241 tcaggttctc gaagaacagc ttgtcgcgcg cctgctcggg cggctcgaag ttgaccgcct
     3301 ccaccttgat cagcgcgaag taccgctccc cttccttcgg ggggcggatc tggcccgaga
     3361 ccgtgtcgcc ggtctgcagg tcgaacttcc ggatctggga cggggagacg tagatgtcgt
     3421 cgggccccgg gaggtagttg tagtccggcg cgcggaggaa cccgaagccg tcgggcagga
     3481 cttcgagcac gccctcggag aagatgtagc cgctctgctc ggtctgcgcc ttcaggatct
     3541 ggaagatcag ctcctgcttc cgcatcccgg tggcaccggc gacgttcagg tccttggcga
     3601 tctgggtgag cttctggatg ctcatgtcct tcagctcgcc gatgttgagc ccgtcgccct
     3661 ggcgccgtgg ctgcgccttg gcagggtcct ggggttcggc cgccgggggc tctgccggca
     3721 cggtctggcc gttggatccg cgcgcctgca tgggttggcg cttgtggttg cttggcatag
     3781 tgtgtgtggc ctgactgact tcttacggta ggtgaatgtg ggttctggaa cagcggggcg
     3841 gaggcccccc gcggggccat cgtgacggga ggaggctaga gacgaacagt ataggaactt
     3901 gcgggcagcc tgtcaaccac cggcccagtg ggatccgcct tctacgttct cattctacct
     3961 tctaccttgg catcccacgt cttgcgttcc gccaccgccg ggccgctcgg gccgtcagta
     4021 ccggctgggg tcttcgccga tgcccaggat gctgcaggcc gccacggtgc gaatcccgag
     4081 cgacgcggcc ttgcgctcga gttcctcgct gcccgcgccc gggttcagcc acacctcccc
     4141 gatgcccttc cgcgccacct ggtcgatcac ctggaggccg atcgccgggc gcacgtagaa
     4201 ggtggccatg tcgatcgcgc cggggacgtc gaggacggtc gggtacgtct tcaacccctc
     4261 gacttccgcc tccgacggcg tgacgggcac gaccgtgaac cccttgtggc tgaacgcgcg
     4321 gacagccttg ttgccgtagc gccgacggtt cgaggacgcg ccgatcaccg cgaccacctt
     4381 ggccatggga tcagtatagc cgaccggcac atcccgctgg gggaggcgcc gtctcccgag
     4441 agcggcgacc gccgatggta caatccccgc cgtgcgcgcc gccgcgatcc tcctcgtcct
     4501 gctctccggc ctcaccttcg gcctgggcct cggcaccccg gcgatcaccg attccgacga
     4561 ggcgtactac gccgaggcga gccgcgagat ggtcgagcgg ggcgactacc tgacaccccg
     4621 gttcaactac cgcgagcgct tccagaagcc ggtgctctac tactgggcgg cggcgggctt
     4681 ctaccaattg ttcggcatcc gcgagtggac ggcccgcgcg gccgcggcgc tctcgggcct
     4741 gggcctggtg ctcctggcct tcgcggccgg gcggcgctgg ttcgacgccg cgacgggact
     4801 gctggccggg gccgttctcg ccaccagctt cggcgccgtg ttcatggcga ggatggcgct
     4861 gcccgacctc ccgctggcct tcttcgtcaa cgcgaccgtc tacaccgccc tggtcgcact
     4921 gttcgaacgc cccgcccgcg tggctcggtg gtgggcggcc tgcggcgccg tcgcgggact
     4981 cggcttcctg accaagggcc cggtcgccat cttgctgccc gccctcgtga tcgccccgct
     5041 cttcgtgttc gagcgcgggt ggcggcgcct gcgcgcccgg cacgtcgcgc tcgcgctggc
     5101 cgccttcgcg ctgaccgccc ttccctggta cgccgcgatg accgccgagc acggcacccg
     5161 gtacctctac ggcttcttcc tgggcgacaa cctggagcgc ttcgcaacca cggcgtataa
     5221 cccgcaccga tcgccgttct tctacgtgcc gatcctcctt ggcggcctgt tgccctggtc
     5281 gccgttcctc ttcctcggca tccgcccgct gctggcggcg tggcgcgccc gccggctgtc
     5341 ccggcgctcg gtcaggctgc tctgctggtc ggtgctcccg ctgctcttct tctcggtctc
     5401 cgtcggcaag cagccgcgct acatcctgcc gatgcttccg ccgctgaccc tggccattgc
     5461 gtggtggatt cgcaccgcca ccctcggccg ccagggggcg gcaggcgagc agcccgcgct
     5521 ggcgcggtgg cttggcttcg cgtcgggcgg gctggtcttc accctcgccg tgctgctgtg
     5581 gagagcccag ccgctgctgc agaacgtctc ccccgcccgc cacctggcgg gcgtcgcggc
     5641 cctggccggc accggcgtcg ccgtgatggc catcgcggcc agccggcgct ggcggctgac
     5701 gcccgctgcc gtggccgtcg ccggggccgc catcgtcctc gccctcaacc aggcggtgct
     5761 ctccgagcgc ggcgcttacc ccgtggagcg gatggcccgc gcggtcacgg ccgagcgcgg
     5821 cgcgggggaa ccggtaggaa cctatcgcgt cttcgtacgg aacctgatct tctacaccgg
     5881 cgtcgagcag gaggacctgt acagcgaagc gcggctgcac gacttcctcg acagccccga
     5941 ccgggtgttg tgcgtgatcc gcgaacacga cctgctggcg ctcgagcagt cgcgggcccg
     6001 cccttaccgc cgtctgcgca ccgtcaccta cttcgaccct gcgacggcga agctccgcgc
     6061 gttgctgtgg ccggatcccg agagggagct gaggacggtg gtgctgatca gcaacaagta
     6121 ggggcgaccc ttgtggtcgc cctcccgcgg atcactcggt ccagatcttt cccgggttga
     6181 ggatcccctt cgggtcgaag gcgtgcttga cccgcttcat gagcgcaatc gtgtcgggcg
     6241 agagttccag gccaaggtag ggcgccttgg tgaagccgac gccgtgctcg ccgctgatcg
     6301 aaccctcgcg ggccacgacc ccttcgaaca gcagcctctc ggcccggcgg gcccgagcca
     6361 tctcggcggg gtcgtcagcg tggaccatga agttgacgtg gatgttgccg tcgcccgcat
     6421 ggccgaagca ggcgatcgtc aggtcgaacc cgtcgcgcag ttcctgcagt aactcgaaga
     6481 gatcggcgat ccggccgcgc gggaccacga cgtcgtggtt caacttggtc ggcgcgatcg
     6541 tcttgagcgc gggggacagc tcgcggcgca ctcgccacag cctgtcgcgc tcctcggatg
     6601 tggacgcccg cagcacctcg gtcgcgccag ccgcaccgca cgcggcggcg acccgcctcg
     6661 cctcctcggc cacggcctcg gcgaggccgt ccacctcgac gagcagcagg gccccggtgc
     6721 cggcgggagc cagctcctcg ccaaggtagc gagcgacggc cgcgagcgac gccgcgtcca
     6781 ccagctccag ggtcgccggg acgacgcgcg agcggatgag ccctgtcacc gcttcgaccg
     6841 ccgctcccgc cgaggggaac gtcgcccgca gcgtggcgcg cgagggcggc agcggcacca
     6901 ggcgcagcac ggcctgcgtg atgatggcca gggtcccctc cgacccgacg agaagctgcg
     6961 tcaggtcgta gccgaccacg ttcttcaccg ccttcgagcc ggtcctgatc acctcgccgc
     7021 cggggagaac cgcctcgagg ccgagcacgt agcggcgcgt cgtgccgtac ttgaacgcgc
     7081 gcgggccgcc ggcgcactcg gcgatgttcc ccccgattac cgatttctcg aggctcgcgg
     7141 ggtcgggcgg gtagaacagg cccacccgct cgacggccgc ctggagcgcg ccggtgatca
     7201 cgttgggctg gacgacggcc agcaggttgt cctgatcgat ctcgaggatg cgattgaacc
     7261 gctccatcga gaccactacg ccgccgcgca ggggcaccga cccgcccgtg tagccggtgc
     7321 cgcccccccg caccgtcagc gggatggccc gggtcgagca ggcctccgcg atcgcagaca
     7381 cctcgagggt cgtgccggga atgacaacga ggtcgggcgg gtggacgcgc gccagggcat
     7441 cctggccgta ggcgtcgagc gcggccgggt cgtcgcgaac ccggtcgcgc cccacgatct
     7501 gacgcatctc ctctacgaag gcgggcggga gcatgtcacg cgaccgcgat ggcacctgcc
     7561 gaagttgccg gaaaggcgct aggcccactc acgcacgagc gtccggccgc cgatctcgat
     7621 ctcgcgcggc tcgcccgcat cgagggccca gcggatgcgg cgcatcccct cgaccacgtc
     7681 gaccgtgctc cccgcgtagc tcaggcgcag gtgcccctcg aggccgaagt cgcggcccgg
     7741 cacggtgacg accctcgcct tgttgagcag gaacatcgac agctcgaacg agtcggccgc
     7801 aatgccgggc ttcaggtagt gcgagaagtc gggcaggcag tagaaggtgc cctgcggctt
     7861 caccacccgc acgcccggca gcttccccag ttcgtcgacg atcacgtcgc ggttgtgctc
     7921 catcgcgcgc cgcagggttt ccacctcgtc ctgcggaccg agcagcgccc cctcggccgc
     7981 cacctggcac aggctcgaca cgcacgaggt ggtctgcgac tggatcttcg acatcgcccc
     8041 gacgatctcg cggctcgcga tggcccagcc gatgcggaag ccggtcatcc cgtacgtctt
     8101 ggcgatcccg ttcacgacga tgaggtggct gccgtcggtc tcgagccggg tgtagtcgta
     8161 gcaggagggc gcggtgacgc cgtcgaacac cagcttgtgg tagatgtcgt ccatgatcgc
     8221 gtggatgccg cgctcctcgc acatccgcac gatactgccc acgaagtcgg gcgagtacac
     8281 cattcccgac gggttgttgg ggctgttgac gacgatggcg cgggtgcggg aggtcacggc
     8341 cgcctccacc cgggcgacgt cgggctccag gctgccgggg tcggacgaca ccacgaccgg
     8401 caccgcgcgg cacatcttca ccatctccgg gtacgagacc cagtaggggg ccaggaggat
     8461 cacctcgtcg cccgcgtcga ggatggcgaa cagcgcgttg aagattgcct gcttggcgcc
     8521 cgaggtgacg atgacgttcc gcggcgcgac ggtccggccg taggtctcct cggtgtagcg
     8581 gatgatcgcc tgcttgaacg acgccaggcc ggtcgtgggc gagtacttga tcttgccgcc
     8641 gtggagcgcg gcggttccct tctcgatcgc cgcctgcggc gcccggttct gcggctcgcc
     8701 gacgccgagg tggacaaccg gctcgccctg ctcgcgcagc aggcgagcct ggtcgttgag
     8761 ggcgagggtc ggggactccg cgatgctccg agcgaactgg ctcacgccca tgtcttcctc
     8821 ctgcgcctac cgggcccggc gcgcgtgcat ccgggcctcg acggcctccg ggaccagccc
     8881 ggcgatcggg ccgccgagcg cgaacacctc ccggatcagc cgggagctga tgtaggtata
     8941 ggcttccgcc ggcatggtgg aatggaaggc cgtgtcaaag acgccgacat tgggcacgcc
     9001 cggcatggcc ttttgcatgg cctcgatgcc ggtgatgttg gccgggttgt gcagcggcgc
     9061 cagggggatg ttctcgcgca gcgcctccat aacgcggtcg cagatgagca cagaaccgga
     9121 aaacttctcg gcaccatgca ccacacggtg gcccaccgcc gcaatgtcgg ccagggactt
     9181 gatgacaccg gttttggggg caaccagctt gtcgagcacc attttgatgg caatttcgtg
     9241 attggcgata tcgacgactt ccttcacctt ctcgccgttg gccggctcat aggcgaggat
     9301 ggagtccttc atgccgatac gttcgaccag accgacagca acacggtgtt cgttggacat
     9361 gtcgagcagt tggtacttga atgaggagct gccgcaattt agtacaaaaa caaacatgat
     9421 tctttccttt gagtaatggc caa
//
LOCUS       DAOPQX010000119         9383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8284_length_9383_cov_4.859670, whole genome shotgun sequence.
ACCESSION   DAOPQX010000119 DAOPQX010000000
VERSION     DAOPQX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8284_length_9383_cov_4.859670"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PKK95_08115"
     CDS             complement(<1..886)
                     /locus_tag="PKK95_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18216.1"
                     /translation="MGADFDYAEEFKTLDLDALKKDIEAVMTTSQDWWPADFGHYGPL
                     FIRMAWHSAGTYRIHDGRGGAGSGEQRFAPLNSWPDNANLDKARRLLWPVKQKYGRKI
                     SWADLMVFAGNCALESMGFKTFGFGGGREDVWEPSEVNWGTEDTWLGDARYSGDRELA
                     NPLAAVQMGLIYVNPEGPGGNPDPVAAAKDIRETFRRMAMSDEETVALIAGGHTFGKT
                     HGAGPATHVGPEPEAAPLEAQGLGWQSSYGTGKGGDTITSGLEVIWTTTPTRWTNNFL
                     WNLFGYEWELTESPAGAHQ"
     gene            complement(928..1515)
                     /locus_tag="PKK95_08120"
     CDS             complement(928..1515)
                     /locus_tag="PKK95_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOC18217.1"
                     /translation="MGSDPAELLRQRGIHVTAQRLAVLRAVACQPHIAAEGVLEVVRS
                     EIGAISLQSVYDALAVLTGAGLIRRIQPAGSPARFEDRVGDNHHHLICRACGRMTDVA
                     CAVGAGPCLEAVDSMGYEVEEAEVIYWGRCPECLARDRASKPGPAAQQRPRSVSQTVT
                     SHHERTSSTWTRKKKLPSRAEPPEDDRTGTGGRTS"
     gene            complement(1579..2571)
                     /locus_tag="PKK95_08125"
     CDS             complement(1579..2571)
                     /locus_tag="PKK95_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC18218.1"
                     /translation="MPRVRWGVLSTAKIGLLKVIPGMQRGELCEVVGIASRDLGAAEK
                     AAGRLGLAKAYGSYQALLDDPAIDAVYIPLPNDLHVPWSLKAIEAGKHVLCEKPIGLT
                     AAEAESFLQASRRAPSIKVMEAFMYRFHPQWQRARQIVREGGIGSLRTIHSFFSYYLD
                     DPANIRNDPSKGGGGLMDIGCYCISLSRFLFGREPARVCGSVEYDPAFGVDRLASGIL
                     DFGDGTSTFTCATQLAPYQRVNIYGTGGRVEIEIPFNAPPDRPCRLWHQHGSEVEEIL
                     LPVADQYTIQGDLFSRAVLENTPVPTPLEDAVANMRAIEAIVESARQGTWLGSV"
     gene            complement(2775..3548)
                     /locus_tag="PKK95_08130"
     CDS             complement(2775..3548)
                     /locus_tag="PKK95_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18219.1"
                     /translation="MRSIALVLMASALTMAPQGHAGEAVAVRYPEGPLNERLVLRAPG
                     GSLLAEGEMSQAVEGALVTTRVVFHFNDGSLHDETVVFSQDRELRFVRDRLVQRGPAF
                     PRSIDMSVDGASGQVTVAYSEGSGQAKHVEREYEIPANLANGMVPVVLKNLGPDRPWP
                     ELSLIVATPSPRLVRLEVKSATADRVAPVQNGEERPATHYVLKAEIGGVAGFFAPLVG
                     RQPPDSHVWIAGGNPPIYLRSDQPFYVGGPLWRMEVAGD"
     gene            complement(3610..6000)
                     /locus_tag="PKK95_08135"
     CDS             complement(3610..6000)
                     /locus_tag="PKK95_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOC18220.1"
                     /translation="MIVRRNAGTAPPRDAAAGRGRYDLLARHSRDILLFIRRADGRLL
                     EVNEAAVKAYGYTRDELLRMAICDLRAPDTRDETAHLMEAADNQGILFETRHVRKDGS
                     EFPVEVSSQGASIEGTATLLSIVRDITGRKQAEEALRFSEEKFAKIFESNPMAICLTR
                     VSDGRLLDANPAFTEIAGYSREEFEGRTASELGLWPKPEDREGAIAELRRHGFFRNRA
                     QSIRVKSGEIREFLASGELLSIAGEPVMLSTWLDITERRRTEKALEESESFYRQTLES
                     IPGMVFTTRPDGYCDYQSQQWVDFTGVPMPEHLGEGWALLLHPDDRPRALAAWRAAVE
                     GRAPYDLEYRVRRHDGHYEWFKVRGVPIHDAAGHVVRWFGVAANIDAMKRVEEELAAA
                     SRAKDQFIAQLSHELRTPLTPAVAAMSLLRTDVRLPADVRGTLAMVSRNLDLEVRLIA
                     DLLDVSRVVSGKLQMEKRPVDVAASLREAAAVVERDLEEKEQALAIEMPAAPYPVMGD
                     AARLQQVFWNLLRNAMKFSPEGSHIVVRARVEPVERCPVEPVTCGGGVKECPLPAPAE
                     GHHGGNLVVEVIDRGSGIHPDILPRLFNAFEQDERARGLGGLGLGLSICKAIVEMHGG
                     SIAARSAGPGLGSTFSVRLPLARCALARPAPSQGENELPAPGPGAPRRLWVLVVEDHA
                     DSAEMMALLLESMGHDAVVAPTAGEALAAVERQRFDLLISDIGLPDASGYDLMRQLAA
                     NGQHIPAIALSGYGTAADLRKSREAGFAEHLVKPLPGPGPLEAAIARLDIGGGRRS"
     gene            6330..6671
                     /locus_tag="PKK95_08140"
     CDS             6330..6671
                     /locus_tag="PKK95_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOC18221.1"
                     /translation="MLRLRSVSAGLALALVAGVAGLLAQSPAPPQAGQASMPKLEFEK
                     YTLPNGLQVILHVDRKLPIVHVNQWFHVGSKNEKAGRTGFAHLFEHMMFQGSKNVPGE
                     YFTHVERAGPT"
     gene            6671..9139
                     /locus_tag="PKK95_08145"
     CDS             6671..9139
                     /locus_tag="PKK95_08145"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC18222.1"
                     /translation="MEGGVNGTTNEDRTNYFATVPSANLEMLMWLESDRLATLLDVTS
                     QDKLDNQRDVVKNERRQGLENQPYGRAFPLLAEAVFPKGHPYSWPVIGSQEDLSAASL
                     DDVKEFFRQYYTPNNLSLAIAGDFDPAEAKRLVEKYFGGLPPGPALDRPARWIPRLDS
                     PRVVEVNDRVSLERVYFGWPAPEYFAEGEAELTLAARVLADGLSSRLNKTLVYDRQLA
                     TGVNAFNATNEIASLFIVMATARPGASLAEIEEVIGQEIARLAKEGPTPAELERAKTK
                     YESEFISGLERIGGFGGKADVLNQYNVFLGDPGKVEEDLQRFRAVTADSLRRAVADWL
                     DTPNRATVRFHPEGSQRAAAPDTLDRAKVPALGEDRPFTAPSVQAGKLPNGLELFVVE
                     RRDLPKVNVSIVTRAGAVADPPGKGGAANLVVRTIDMGTPARSALEIEDALGALGTTL
                     GGFSGRESARLGFDVLTRNLSPALAIVAEVVQQPTFPENEIAREKKRQLDAIAQQDRS
                     PNALAARIAPMLAFGRDHPYGRPVSGLKATVEALTRADLAAFHAARWKPGSTALVFVG
                     DVTLAQATELARQHFGGWSGGAAPAVQIPAPAPAAGARLFVVDRPDSAQTVVAQWLQA
                     PERRSPDYDALSLVDAVWGGGGFGTRLNLNLREDKGYSYGVFSNFDPMSRAGQWIASG
                     GVQTDKTAAAVAEFDKELKDLVSGRPIGAEELEHAKTRRVRGYAQQFESLGRIGQQVG
                     DLWTLGLPMSELQREYEATRQVSLDAVLAAVKKYVRPEASGLLLVGDRAKIEQGLRDL
                     QLGEIAMLDIEGRPAATTNQAAGR"
BASE COUNT         1412 a         3353 c         3206 g         1412 t
ORIGIN      
        1 actggtgggc gcccgccggg ctctcggtca gctcccactc gtacccgaac aggttccaga
       61 ggaagttgtt ggtccacctg gtgggcgtgg tcgtccagat cacctcgaga ccgctggtga
      121 tcgtgtcgcc ccctttgccc gtgccgtagc tgctctgcca gcccaggccc tgcgcctcga
      181 gcggagccgc ctcgggctcg gggccgacgt gcgtcgccgg gcccgcgccg tgcgtcttgc
      241 cgaaggtgtg gccgcccgcg atcagcgcca ccgtctcctc gtcgctcatc gccatgcggc
      301 ggaacgtctc gcgaatgtcc ttcgcggccg caaccggatc ggggtttccg ccaggccctt
      361 ccgggttgac gtagatcagg cccatctgga ccgccgcgag cgggttcgcc agctcacggt
      421 cgccgctgta gcgcgcgtcg ccgagccagg tgtcctcggt cccccaattc acctcggagg
      481 gctcccacac gtcctcgcgc ccgccgccga acccgaacgt cttgaagccc atcgactcga
      541 gcgcgcagtt gccggcgaac accatcaggt cggcccacga gatcttccgg ccgtacttct
      601 gcttgaccgg ccagagcagg cgccgcgcct tgtcgaggtt cgcgttgtcg ggccagctgt
      661 tgagcggcgc gaagcgctgc tcccccgacc cggcgccgcc gcgaccgtcg tggatgcgat
      721 aagtgccggc gctgtgccag gccatccgga tgaataacgg cccgtagtgg ccgaagtcgg
      781 ccggccacca gtcctgcgac gtggtcatca ccgcctcgat gtccttcttc agggcgtcga
      841 ggtcgagcgt cttgaactcc tccgcgtagt cgaagtccgc acccatcggg ttgcgggccg
      901 gagggttctg gtgcagagcc tggaggttca gctggttcgg ccaccagtcc ctgttcgatc
      961 gtcctctggg ggctcggccc gcgaggggag cttctttttc cttgtccatg tagatgatgt
     1021 cctctcgtgg tgtgaagtaa cggtctggct gacactgcgg gggcgttgtt gcgccgcggg
     1081 gcccggcttc gacgctcggt cgcgcgcgag gcactcggga cagcggcccc agtagatcac
     1141 ctcggcctcc tcgacctcgt agcccatgct gtccacggcc tcgagacagg gacccgcgcc
     1201 gaccgcgcat gccacgtccg tcatgcggcc gcacgcccgg cagatcaggt gatggtggtt
     1261 gtcgccgacg cggtcctcga agcgcgcggg cgacccggcg ggctgaatcc gccggatgag
     1321 gcccgcgccc gtcaggacgg caagggcgtc gtacaccgac tggagcgaga tggcgccgat
     1381 ctcggagcgg acgacctcca gcacgccttc ggcggcaatg tgcggttgac aggcgacggc
     1441 ccgcaggacg gccagccgct gcgccgtcac gtggatgccg cgctgccgga gaagctcagc
     1501 ggggtcggaa cccatcggga gagtctaccc caggaatttg gaatcactca agagtttgat
     1561 taggccagaa cactccgctt acacgctccc gagccaggtc ccctgccggg cgctctcgac
     1621 gatcgcctcg attgcgcgca tgttggcgac ggcatcctcg agcggagtcg gcaccggcgt
     1681 gttctcgagc accgcgcgcg agaagagatc cccctggatc gtgtactggt cggcgaccgg
     1741 gagaagaatc tcctccacct ccgagccgtg ctggtgccac aggcggcacg ggcgatcggg
     1801 cggcgcgttg aacggaatct cgatctccac ccggccgccg gtgccgtaga tgttcacgcg
     1861 ctgatacggt gccagttgcg tcgcgcaggt gaaggtggac gttccgtccc cgaagtcgag
     1921 gatgcccgag gccagtcgat ccacgccgaa cgccgggtcg tactcgacgc tgccgcagac
     1981 gcgtgccggc tcgcgcccga acaggaagcg cgagagcgag atgcagtagc agccgatgtc
     2041 catcaggccg ccgccgccct tgctcgggtc gttgcggatg ttcgccgggt cgtcgaggta
     2101 gtacgagaag aacgagtgaa tcgtccgcag cgacccgatc ccgccctcgc gcacgatctg
     2161 ccgcgcgcgc tgccactgcg ggtggaagcg gtacatgaac gcttccatca ccttgatcga
     2221 cggggcgcgc cgcgatgcct gcaggaacga ttcggcctcg gccgcggtca acccgatcgg
     2281 cttctcgcac agcacgtgct tccccgcctc gatcgccttc agcgaccagg gcacgtggag
     2341 gtcgttcggc agcgggatgt agacggcgtc gatcgcggga tcgtccagca gcgcctggta
     2401 gctgccgtac gccttcgcga ggcccaggcg gccggcagcc ttctcggcgg ccccgaggtc
     2461 gcgggacgcg atccccacga cctcgcacag ctccccccgc tgcatgccgg ggatcacctt
     2521 cagcaggccg atcttcgcgg tgctcagcac tccccaccgg actcgcggca tgcgcggctc
     2581 ccttccctac accgctcctc ggttccgctg gcaaggacct gccccttcgc gggtcctgcc
     2641 gcggggctcc ccccgctcgg cttcgcgcga aaagcgcttg tcacgcgtgt cgcgctccag
     2701 gcgagcgggg gccctccgcc ccgcgtcacc cgctcacggc cttgacagta tgggagccac
     2761 tataccccag ccgttcagtc gccggcgacc tccattcgcc acagcggtcc gcccacgtag
     2821 aacggctggt cggagcgaag gtagataggt gggttgcccc ccgcaatcca gacgtgcgag
     2881 tcgggaggct gccggccgac cagcggtgcg aagaagccgg ccacgccgcc gatctcggcc
     2941 ttcaggacgt agtgcgtcgc cggccgttcc tctccattct ggactggggc cacgcgatcg
     3001 gcagtggcgg acttgacctc gagccgcacc agccgaggcg acggggtggc gacgatcagc
     3061 gagagttccg gccacgggcg gtccggcccg aggttcttca gcacgacggg caccatgccg
     3121 ttcgccaggt tcgcggggat ctcgtactcc cgctcgacgt gcttcgcctg gccgctccct
     3181 tccgagtagg cgacggtcac ctgcccgctg gcgccgtcta ccgacatgtc gatcgatcgc
     3241 ggaaacgccg gcccgcgctg caccaggcgg tcgcggacga atcggagttc ccggtcctgt
     3301 gagaagacga ccgtctcgtc gtggagcgag ccgtcgttga aatggaagac gacgcgggtg
     3361 gtgacgagcg ccccctccac cgcctgtgac atctcgccct cagcgagaag ggagccgccc
     3421 ggcgcgcgca ggacgagacg ttcgttcaga gggccttccg ggtagcgcac cgccaccgcc
     3481 tcgccagcgt ggccctgcgg agccatcgtc aacgccgacg ccatcagcac aagagcgatg
     3541 ctccgcatcc tgcctcctgc cgaggagcag gaactgcagg tctcgtaccg aagaacgcgc
     3601 aagggaaggc tagctccggc gcccgccccc gatgtccagc cgggcaatcg ccgcctccag
     3661 cggcccgggc cccggcagcg gcttgacgag gtgctcggcg aacccggcct cgcggctctt
     3721 gcggagatcg gcggccgtgc cgtaaccaga cagggcgatc gcggggatgt gctggccgtt
     3781 cgccgcgagc tgccgcatca ggtcgtagcc gctggcgtcg ggcagcccga tatccgagat
     3841 caggaggtcg aagcgctggc gctcgaccgc ggcgagggcc tcgccggcgg tgggagcgac
     3901 gaccgcgtcg tggcccatgc tctcgagcag cagcgccatc atctcggccg agtcggcgtg
     3961 atcctcgacc accaggaccc aaaggcggcg tggcgcaccg gggccgggtg ccgggagctc
     4021 gttctcccct tgggaaggcg caggcctcgc cagcgcgcaa cgcgccagcg gcaggcgcac
     4081 cgagaaggtc gagccgaggc cgggccccgc gctccgcgcc gcgatgctgc cgccgtgcat
     4141 ttccacgatc gccttgcaga tgctcaggcc gagcccgagg ccgcccagtc cccgcgcccg
     4201 ttcatcctgc tcgaacgcgt tgaagagcct gggcaggatg tcggggtgga tgccgctccc
     4261 ccggtcgatc acctccacca ccaggttgcc gccgtggtgc ccttcggccg gcgcaggcag
     4321 cgggcactcc ttcaccccgc ccccgcacgt cactggttcc accgggcagc gctcgaccgg
     4381 ctccacccgc gcccgcacca cgatgtgact gccctcggga ctgaacttca tcgcgttgcg
     4441 aagcaggttc cagaacacct gctgaagccg cgcggcatcg cccatgacgg gatacggggc
     4501 ggcgggcatc tcgatggcga gcgcctgttc cttctcttcc aggtcgcgct ccacgacagc
     4561 agccgcctcg cgaagggagg cagcgacgtc caccgggcgc ttctccatct gcagcttgcc
     4621 cgacaccacc cggctcacgt cgagcaggtc ggcgatgagg cggacttcga gatcgaggtt
     4681 gcggctgacc atggcgagcg tgccccgcac gtcggctggc agccgcacgt cggtccgcag
     4741 cagcgacatg gccgccacgg ccggcgtcag cggcgtgcgc agctcgtggg agagctgcgc
     4801 gatgaactgg tccttcgcgc ggctcgccgc cgccagctcc tcctcgaccc gcttcatcgc
     4861 gtcgatgttc gccgcgacgc cgaaccagcg gacgacgtgc ccggcggcat cgtggatcgg
     4921 gacgccccgc accttgaacc actcgtagtg accgtcgtgg cggcgcaccc ggtactccag
     4981 gtcgtacggc gccctcccct cgaccgccgc ccgccaggcc gccaacgcgc gcgggcggtc
     5041 gtcggggtgc agcagcagcg cccacccctc gccgaggtgc tcgggcatcg gcacgcccgt
     5101 gaagtccacc cactgctggc tctggtagtc gcagtacccg tcgggccgcg tcgtgaagac
     5161 catgccgggg atcgactcca gcgtctggcg gtagaacgac tcgctctcct ccagcgcctt
     5221 ctcggtccgg cggcgttcgg tgatgtcgag ccaggtggag agcatcactg gctcccccgc
     5281 gatcgacagc agctcgcccg aggccaggaa ctcgcgaatc tcgcccgact tcacgcgtat
     5341 gctctgggcc cggttgcgga agaagccgtg ccgccgcagc tcggcaatcg ccccctcgcg
     5401 gtcctccggc ttcggccaca ggcccagctc tgaggcggtg cggccctcga actcctcgcg
     5461 gctgtagccc gcaatttcgg tgaaggcggg gttcgcgtcg aggagccggc catcggagac
     5521 gcgcgtgagg cagatggcca tcgggttcga ctcgaagatc ttggcgaact tttcctccga
     5581 aaaccgcagc gcctcttcgg cctgcttgcg cccggtgatg tcgcggacga tgctgagcag
     5641 cgtggcggtt ccctcgatcg aggctccctg cgagctcact tcgaccggga actcgctgcc
     5701 gtccttccgg acgtgccggg tctcgaacag gatgccctgg ttgtcggcgg cctccatcag
     5761 gtgcgctgtc tcgtcccgcg tgtcgggcgc gcgaaggtcg cagatcgcca tccgcagcag
     5821 ctcgtcgcgc gtgtagccgt aagccttcac cgcggcctcg ttgacctcga ggagccgtcc
     5881 gtctgcgcgg cgaatgaaca ggaggatgtc gcggctgtgt cgggcgagca ggtcgtaacg
     5941 gcccctgccc gccgcggcgt cgcgaggagg ggcggttccg gcgttccggc gcacaatcat
     6001 gttggcctcg aagacgcggg gatctacctg gaattctccg cgcgtcgagg caacgggaac
     6061 ccggggtcac tgtcggctga gatgatggcg aagcgtcgac cggcggaacg cacattctag
     6121 ggggacgagg cccctttcgc aacctcgacc ggcgacctcg acgggcggcc tcgcccgcag
     6181 gtcaccgccg caggacgggc ggtgggtcat gggattacca tgggatttcc accgcatcac
     6241 caggaccgcg cgtaaggcca cggccaccat cgggaaggcc accaggacag gggcccgtca
     6301 ccgctcggag gaattcagcc ggaggaagta tgttgcgact cagaagcgtc tcggcagggc
     6361 tcgccctcgc tctcgtcgcc ggcgtcgcag ggctcctcgc gcagtcgccg gcgccgccgc
     6421 aggccggcca ggcgtcgatg ccgaagctcg agttcgagaa gtacaccctg cccaacggcc
     6481 tgcaggtgat cctccacgtg gatcgcaagc tgccgatcgt gcacgtgaac cagtggttcc
     6541 acgtcggctc gaagaacgag aaggcgggcc gcaccggctt cgcccacctc ttcgagcaca
     6601 tgatgttcca gggatcgaag aacgtccccg gcgagtactt cacgcacgtg gagcgcgccg
     6661 gcccaacgtg atggaaggcg gcgtcaacgg gacgaccaac gaagaccgga cgaactactt
     6721 cgcgaccgtg ccgtcggcaa acctcgagat gctgatgtgg ctcgagtcag accggctcgc
     6781 aacgctgctc gacgtcacct cgcaggacaa gctcgacaac cagcgcgacg tggtcaagaa
     6841 cgaacgccgc caggggctcg agaaccagcc ctacggccgc gccttcccgc tgctggcaga
     6901 ggcggtcttc cccaagggcc acccctactc gtggccagtc atcggcagcc aggaagacct
     6961 ctccgccgcc tcgctcgacg acgtgaagga gttcttccgg cagtactaca cgcccaacaa
     7021 cctgtcgctg gcgatcgcgg gcgacttcga tcccgccgag gccaagcggc tcgtcgagaa
     7081 gtacttcggg gggctccccc cggggcccgc gctcgatcgg ccggcgcgct ggatcccccg
     7141 cctcgacagc ccgcgggtgg tcgaggtgaa cgaccgcgtg tcgctcgagc gcgtgtactt
     7201 cggctggccg gcgcccgagt acttcgccga gggcgaggcc gagctgacgc tggcggcgcg
     7261 ggtgctcgcc gacggcctgt cctcgcggct caacaagacc ctcgtctacg accggcagct
     7321 cgcgaccggc gtgaacgcct tcaacgcgac caacgagatc gccagcctgt tcatcgtgat
     7381 ggcgacggcg aggcccgggg cttccctcgc ggagatcgag gaggtgatcg ggcaggagat
     7441 cgcgcggctc gcgaaggaag gcccgacgcc ggccgagctc gagcgggcga agacaaagta
     7501 cgagtcggag ttcatctcgg gcctcgagcg catcggcggc ttcggcggca aggccgacgt
     7561 actaaaccag tacaacgtct tcctcggcga ccccgggaag gtggaggagg acctccagcg
     7621 gttccgcgcc gtcacggccg attcgctgcg gcgggccgtc gccgactggc tcgatacgcc
     7681 gaaccgcgcc accgtccggt tccaccccga ggggtcgcag cgcgcggcgg cgcccgacac
     7741 cctcgatcgg gcgaaggtgc cggcgctcgg cgaggaccgg cccttcacgg cgccgtcggt
     7801 ccaggccggc aagctcccga acggcctcga gctgttcgtc gtcgagcgcc gcgacctgcc
     7861 gaaggtgaac gtgtcgatcg tcactcgcgc gggggcagtc gccgatccac ccgggaaggg
     7921 gggcgcggcc aacctggtgg tccgcacgat cgacatgggc acgcccgcgc ggagcgcgct
     7981 cgaaatcgag gacgcgcttg gcgcgctggg gacgaccctc gggggattct ccggtcgcga
     8041 aagcgcgcgc ctcgggttcg acgtcctcac ccgcaacctc tcgcccgcgc tcgccatcgt
     8101 cgccgaggtc gtgcagcagc cgaccttccc cgagaacgag atcgcgcgcg agaagaagcg
     8161 gcagctcgac gccatcgcgc agcaggaccg aagcccgaat gccctcgcgg cgcgcatcgc
     8221 cccgatgctg gcgttcggcc gcgaccaccc gtacggccgc ccggtttcgg gcttgaaggc
     8281 cacggtcgag gcgctgacgc gcgccgacct cgcggccttc cacgcggcgc gctggaagcc
     8341 gggatcgacg gcgctggtct tcgtgggcga cgtgacgctg gcgcaggcaa ccgagcttgc
     8401 caggcagcac ttcggcggct ggagcggcgg cgcggcgccc gccgtgcaga tccccgcgcc
     8461 cgcgccggcg gccggcgccc ggctcttcgt ggtcgatcgc cccgactcgg cgcagaccgt
     8521 cgtggcccag tggcttcagg cccccgaacg ccggtcgccc gactacgacg ccctctcgct
     8581 ggtggacgcg gtgtggggcg gcggcgggtt cgggacgcgg ctcaacctga acctccgcga
     8641 ggacaagggc tactcgtacg gcgtcttctc caatttcgac ccgatgtcgc gcgcgggaca
     8701 gtggatcgca agcggcggcg tgcagaccga caagaccgcc gcggcggtgg ccgagttcga
     8761 caaggagctg aaggacctgg tgagcggccg ccccatcgga gccgaggagc tcgagcacgc
     8821 gaagacgcgc cgcgtgcgcg gctacgcgca gcagttcgag tcgctcggcc ggatcggcca
     8881 gcaggtcggc gacctctgga cgctcggcct gccgatgagc gaactgcagc gcgagtacga
     8941 ggccacccgg caggtgtcgc tcgacgcggt gctcgccgcc gtgaagaagt acgtacgccc
     9001 cgaggcctcg gggctgctgc tggttggaga ccgcgcgaag atcgaacagg ggctgcgcga
     9061 tctgcagctc ggcgagatcg cgatgctgga tatcgagggg cggcccgccg ccaccacgaa
     9121 ccaggcagcc ggccgctgat cgctcggacg gaggggccgc ctccacagga agccggggcg
     9181 gctcctcgac tccggcgctc ttgcgttgag caaaggcgtg catgttcgcg ggtcctgccg
     9241 cggggctccc ccgctcggct tcgcgcgaag agcgcttgtc acgcgtgtcg cgctccaccc
     9301 ttcgacttcg ctcagggtgc ggtgaggttt gtcgaaccgc aggcgagcgg gggccctccg
     9361 ccccgcgtca cccgctcacg gcc
//
LOCUS       DAOPQX010000120         9342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8332_length_9342_cov_4.142673, whole genome shotgun sequence.
ACCESSION   DAOPQX010000120 DAOPQX010000000
VERSION     DAOPQX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8332_length_9342_cov_4.142673"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="PKK95_08150"
     CDS             <1..1825
                     /locus_tag="PKK95_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18223.1"
                     /translation="GPLNKRTEISPRFNAKLTLQPTSSDTIILGGQYDQYNVDGRVGW
                     WSSAQTIYDATVKEDAPEWVWNAQYRRVFGSSSLLEAKFTGYSGYYYLDPVNPDPPSF
                     DGGTGEYGGGGGGIYYADRGRNQVNVSFTRYAQKFGSHSLKFGAEIERSRARSQSQPY
                     GPAGFYIYSYYGVPYGQYSYSYDLPARNKRMSAFAQDQWSVGRLTMNLGVRLDHIRGS
                     GDNIDYDVYKPKASWGPRLGAAYDLTGKGNTVLKGFWGRYFEGGSAGFFEYSLPPGVS
                     DFSVRPYNEDGTLSDTVEVLTPGFLYGMASNVRHPSTDEFNLSFEQQITRTLRFTVTG
                     IYRTWGNFINNVVPGAIWSPVTFRNDLTGQDFTGYYWVNREDTATSVMTQNIKGYKFM
                     AADGSTIATADPERNYKGLMFVLSRSLRSNFGFQASYVLSKSEGNMDNSGWGDWLGGT
                     RWMSPNQAIVNNRGELSNSRTHEIKIHTSYKVPYVDVMLGLSYTGMSGYPYTPYAQFG
                     EGDLNVIGSSRRRIYLKERGSDRNDFYHVLDLRAEKVFNVSGQRFGVYADFNNLTNRA
                     GVISRNTRYPYTTIGGEQVLYGWPTEIQTPRQVTLGARWSF"
     gene            complement(1948..4347)
                     /locus_tag="PKK95_08155"
     CDS             complement(1948..4347)
                     /locus_tag="PKK95_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC18224.1"
                     /translation="MPERSSLGRLVRRLPRRAFAAVALLLAIAIAAAWLAWPRPTRSP
                     GGIELGGLPAGVSRDRLNLVLVTLDTTRADRIGAYGARDVETPAIDALAREGVLFEQA
                     VAVAPLTLPVHSTLFTGKFPPEHGVRDNGGFFLGAEQVTLAEVLKEKGYRTGGFVAAY
                     VLDSKWGVDQGFDTYFDDFDLSKTKVMSIASIQRPGNEVVDKVLPWLDGAKEAPFFAW
                     VHLYDPHTPYRPPEPYATRYEQHPYNGEVAFADSQVGRIVERLKTLGLYDRTIVAVMG
                     DHGESLGDHGEATHGFFIYNPVTHVPFVIRAPFGLTASRRVADPVRSVDLMPTLLDLL
                     GVETPAGVSGASLVPLMTGARREMGLDAYSESMYPLHHYGWSDIRALRAGRYKVIDAP
                     RPELYDLERDPGERTNLFGERRAMADTMLAQLRRLESRFSKTEAALPASDADPEARAR
                     LAALGYVGSFVASAADSRTGRADPKDKIGLFNQLGRAMDLANDNDDEAAPPSQKMIAL
                     LEDVIEQDPQVIDAWFTLGTHYLRHGEPRKSLEYFQRALSLKPDYDLAVINLARAYRA
                     LGDDEAALAGFERYLQIDPRDAYVYYQVGELWLDRGDAARAEAAFRRALEIDPMVAGA
                     KNALGVIALERGDLAGAERLVREAIAAKPDVRLAHFNLALLAEKRGDLAGAEREYLEE
                     LKQHPESYKAAFNLSLLYEQVGDREGQIEALEQSIVSNPRFAEGHFYLAKAYLDAGSN
                     LDEAIRVARKGLELGPQSEYAALGHYVLADLLNRKGLAREAAVQVSLGRAAEAKGRGG
                     K"
     gene            4466..5335
                     /locus_tag="PKK95_08160"
     CDS             4466..5335
                     /locus_tag="PKK95_08160"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOC18225.1"
                     /translation="MPQTRPRPSRAEPREVVELRQLKERQPELAPAVDLQVALIELQR
                     RVQARVPVPWIEFAPDWLKAQQAAGRPLLRFEEIPLDWTDVRLVFRQTADVLRRFEAL
                     EPADYRALQALGRDGHSLEPFVADWFNRAADPARAGSPPDLPVAVSPEALEQVLALTL
                     RPFLERCAEAVRQRADFSAWHLASCPLCGGEPELATLDPQGERCLVCGRCTTEWRFER
                     GVCPFCRNDDRSLLPSFGSRDGLYRLEACDVCRRYLKTFDVRAGRRHVLLAVDSVATL
                     PLDAAAIQRGYRG"
     gene            complement(5449..6519)
                     /locus_tag="PKK95_08165"
     CDS             complement(5449..6519)
                     /locus_tag="PKK95_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOC18226.1"
                     /translation="MSLRSHPTWAALAAALCVSFGLSVSLPASVAAAAQSRGTSVQKS
                     TTKKTSTVRRAKSRRTARSKAAAARRARLARARAAAIREAQLPRYKTDATGTLVPDLH
                     AAAAIIYNPETKQVLWEEHSQDKRSIASITKVMTAVIISETDPDPARMVTIERADVYA
                     ASTTHLRANYVVSVDDLMHLLLISSDNAAARALARVSPWGADGFVRRMNEKAVELGLQ
                     STAYADPSGLDAANVSSAYDMARLIAYAAGDEWISEIMRKKEHQLVLGKRARTIHSTN
                     RLLANSELQPNVLGGKTGFINRAGYCLVTLLKLPEVNQPVAVVVLGARSNAGRFTEAR
                     NLLGWVSGKAKELLAGKDEKPE"
     gene            complement(6777..7010)
                     /locus_tag="PKK95_08170"
     CDS             complement(6777..7010)
                     /locus_tag="PKK95_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOC18227.1"
                     /translation="MPHVTLYAVPGCPRCEEVRQFLRGRGIAFDEIDVQTNPLAFHRT
                     MIFSGTPAVPALDVDGRVVVGVDPEGLEELLRD"
     gene            complement(7104..8378)
                     /locus_tag="PKK95_08175"
     CDS             complement(7104..8378)
                     /locus_tag="PKK95_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC18228.1"
                     /translation="MWRRVAILCAIAMALAAAGAVTGGPGGAKGAPEDAVAALMRDPS
                     LQAALAGVEAAEPALLDDQVAFTEIPAPPFAERVRAARLKQVFEALKLRNVRIDAAGN
                     VIGERPGLAARPNLVVAAHLDTVFPEGTRVKVTREGPILRGPGIADDGRGLAVLAGVA
                     RSLDRGGVRTPGTITFVANVGEEGLGDLRGVRHLIGHELKGRVDAFVSIDGCGHGITN
                     VAVGSRRYRVTFKGPGGHSFGAFGTVNPVHALGRAVARVADFEVPRSPRVTFNVGRIG
                     GGTSVNSISEEAWFEIDMRSSDGAALASIDATFKGVVEQAVRQENERWETGRVGVQVE
                     PVGNRPAGRTPEDSSIVKSALAVNRALGLPARLGEGSTDANIPIALGIPAITVGGGGR
                     STGAHTLAESFDSTDSWKGTSRVTALVFALAR"
     gene            8619..8825
                     /locus_tag="PKK95_08180"
     CDS             8619..8825
                     /locus_tag="PKK95_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18229.1"
                     /translation="MSLVRLLGFATLQCGCVVGRYLELSSSREVVYVEEKGVECAMHH
                     HRRNHALPRRFSGAVPVVVVAKAS"
     gene            8837..>9342
                     /locus_tag="PKK95_08185"
     CDS             8837..>9342
                     /locus_tag="PKK95_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOC18230.1"
                     /translation="MTARPTPYASLRHPNGSSLDFFLGDCLELLPCLASERVDVVVTS
                     PPYNLGVKYRSYQDTVPRDRYLEWTGEWVQAVRAIMGPGASLFLNVGAKPKDPWVAMD
                     IAQAVRPHLRLQNTVLWVKSIVIERAAAGEDAMPLVPTGLPAGRLDGAVRAALLCRLP
                     TVPFLRPAG"
BASE COUNT         1442 a         3263 c         3174 g         1463 t
ORIGIN      
        1 cgggcccctc aacaagcgca ccgagatcag cccgcgcttc aacgccaagc tgacgctgca
       61 gcccacctcg tcggacacca tcatcctcgg tggccagtac gaccagtaca acgtggacgg
      121 ccgcgtgggc tggtggtcgt cggcccagac gatctacgac gcgacggtca aggaggacgc
      181 cccggagtgg gtgtggaacg cgcagtaccg cagggtgttc ggctccagct ccctgctcga
      241 ggcgaagttc accgggtaca gcggctacta ctacctcgac cccgtcaacc ccgatccgcc
      301 cagcttcgac ggcggaaccg gcgagtacgg cggcggaggc ggcgggatct actacgccga
      361 ccgcggccgc aaccaggtca acgtgtcctt cacgcgctac gcccagaagt tcggcagcca
      421 ctcgctcaag ttcggcgccg agatcgagcg cagccgtgcg cgcagccagt cgcagcccta
      481 cgggccggcg ggtttctaca tctacagcta ctacggggtg ccgtacggcc agtacagcta
      541 ttcgtacgac ctccccgccc ggaacaagcg gatgtcggcc ttcgcccagg accagtggag
      601 cgtcgggcgc ctgacgatga acctcggcgt gcgcctcgat cacatccggg gcagcggcga
      661 caacatcgac tacgacgtct acaagccgaa ggcgtcgtgg ggacctcgcc tcggcgcggc
      721 ctacgacctg acgggcaagg gcaacaccgt gctgaagggg ttctggggcc ggtacttcga
      781 gggcgggtcg gccgggttct tcgagtactc gctcccgccc ggggtctccg acttcagcgt
      841 ccggccgtac aacgaggacg gcacactcag cgacaccgtc gaggtcctca cgcccggctt
      901 cctgtacggg atggcgagca acgtgaggca cccgagcacc gacgagttca acctctcgtt
      961 cgagcagcag atcacgagga cgctgcgctt caccgtgacc ggcatctatc ggacgtgggg
     1021 gaacttcatc aacaacgtgg tcccgggcgc gatctggagc ccggtcacgt tccgcaacga
     1081 cctcaccggg caggacttca cgggatacta ctgggtgaac cgtgaggaca ccgcgacgag
     1141 cgtgatgacc cagaacatca agggctacaa gttcatggcg gccgacggca gcaccatcgc
     1201 cacggccgac cccgagcgga actacaaggg cctgatgttc gtcctcagca ggagcctgcg
     1261 cagcaacttc gggttccagg cctcgtacgt cctctcgaaa tccgagggga acatggacaa
     1321 cagcgggtgg ggcgactggc tgggcggtac acgatggatg tcgcccaacc aggcgatcgt
     1381 caacaacagg ggggagctca gcaattcacg cacacacgag atcaagatcc acacgtcgta
     1441 caaggtgccg tacgtggacg tgatgctggg tctctcgtac accggcatga gcggctaccc
     1501 gtacaccccc tacgcccagt tcggcgaggg cgacctgaac gtcatcggat cgagccggcg
     1561 ccggatctac ctgaaggagc gaggcagcga ccgcaacgac ttctatcacg tgctcgatct
     1621 cagggccgag aaggtgttca acgtctcggg ccagcgtttc ggggtctacg ccgacttcaa
     1681 caacctgacg aacagggccg gcgtgatctc gaggaacacg aggtacccgt acacgaccat
     1741 cgggggcgag caggtcttgt acggctggcc gaccgagatc cagaccccgc gccaggtcac
     1801 gctgggcgcg aggtggtcgt tctagaagag gcttagggct caaggctcaa ggctcaaggc
     1861 tcacggctca cggcgctgca gagcgggccg ggcgaacgag attcgcccgg cccgcctttt
     1921 ttcctgctga tccccgatcc ctgatcctta cttccctccc ctccccttcg cctctgccgc
     1981 ccgcccgagc gacacctgca ccgccgcctc gcgcgccagc cccttgcggt tcagcaggtc
     2041 ggcgagcacg tagtggccga gcgccgcgta ctcggattgc ggccccagct ccagcccctt
     2101 gcgcgccaca cggatcgcct cgtcgaggtt cgagccggcg tcgaggtacg ccttcgccag
     2161 gtagaagtgc ccctcggcga agcggggatt gctgacgatc gactgctcca gcgcctcgat
     2221 ctgcccctcg cggtccccca cctgctcgta gagcagcgag aggttgaagg cagccttgta
     2281 gctctcgggg tgctgcttca gctcctcgag gtactcgcgc tcggcgccgg cgaggtcgcc
     2341 gcgcttctcg gcgagcagcg cgaggttgaa gtgcgcgagc cggacgtctg gcttggcggc
     2401 aatcgcctcg cgcaccaggc gctcggcacc ggccaggtcg ccgcgctcga gcgcgatgac
     2461 gccgagcgcg ttcttcgcgc ccgccaccat cgggtcgatc tcgagcgctc tcctgaaggc
     2521 cgcctccgcg cgcgcggcgt caccgcgatc gagccagagc tcgccgacct ggtagtacac
     2581 gtacgcgtcc cgcggatcga tctggagata ccgctcgaac ccggccagcg ccgcctcgtc
     2641 gtcgccgagc gcccggtacg cgcgcgccag gttgatcacc gccaggtcgt aatcgggctt
     2701 gaggctgagc gccctctgga agtactccag cgacttgcgc ggttcgccgt gccgcaggta
     2761 gtgcgtgccc agcgtgaacc aggcgtcgat gacctgcggg tcctgctcga tgacgtcctc
     2821 gagcagcgcg atcatcttct gcgacggcgg tgcggcctcg tcgtcgttgt cgttggcgag
     2881 gtccatcgcg cggccgagct ggttgaacaa cccgatcttg tccttcgggt cggcacgccc
     2941 cgtccgggag tcggcggcgg aggcgacgaa cgagccgacg tagccgagcg cggcgagacg
     3001 ggcccgcgcc tcggggtccg cgtccgaggc cgggagtgcc gcctcggtct tcgagaatcg
     3061 cgactcgagc cgccgcagct gcgcgagcat cgtgtcggcc atcgcccggc gctcgccgaa
     3121 gagattggtg cgctcgcccg ggtcgcgctc gaggtcgtac agctcgggcc tgggggcgtc
     3181 gatcaccttg tagcggccgg cccgaagggc gcggatgtcg gaccagccgt agtggtgcag
     3241 cgggtacatc gactccgagt acgcgtcgag gcccatctcg cgccgcgccc ccgtcatcag
     3301 cggcaccagg ctcgcacccg agacgccggc cggcgtctcg acgccgagca ggtcgagcag
     3361 tgtcggcatc aggtccaccg agcgcaccgg gtcggccacc cggcgcgagg cagtcaggcc
     3421 gaagggcgcc cggatgacga acgggacgtg cgtgaccggg ttgtagatga agaacccgtg
     3481 cgtcgcctcg ccgtgatcgc caaggctctc accgtggtcg cccatcaccg ccacgatcgt
     3541 ccggtcgtac agtccgagcg tcttcaggcg ttcgacgatg cgcccgacct ggctgtcggc
     3601 gaacgccacc tcgccgttgt aggggtgctg ctcataccgg gtggcgtagg gctcgggagg
     3661 ccggtagggc gtgtgcgggt cgtagagatg cacccaggcg aagaagggcg cctcctttgc
     3721 cccatccagc cagggcagca ccttgtccac gacctcgttg cccggccgct ggatcgacgc
     3781 gatcgacatg accttcgtct tgctcaggtc gaagtcgtcg aaataggtgt cgaacccctg
     3841 gtcgacgccc cacttcgagt cgagcacgta ggcggcgacg aacccgccgg tgcggtagcc
     3901 cttctccttc aggacctccg ccagcgtcac ctgctccgcc ccgaggaaga agccgccgtt
     3961 gtcgcgtacc ccgtgctcgg gcgggaactt gccagtgaaa agcgtggaat ggaccggcag
     4021 ggtgagaggg gccacggcca ccgcctgctc gaacagcacc ccttcccggg cgagcgcgtc
     4081 gatcgccggg gtttcgacgt cgcgggcccc gtacgccccg atccggtctg cgcgcgtggt
     4141 gtcgagcgtg acgaggacga ggttcaggcg gtcgcgggag acgccggccg gcagaccgcc
     4201 cagctcgatg ccgcccggac tacgggtcgg ccgaggccag gcgagccagg cggccgcgat
     4261 ggcgatcgcg aggagcagcg cgacggccgc gaaggcccgt cgagggagcc gccggacgag
     4321 ccgcccgagg gaggaccgct ctggcatggg gccatgatag gggacaagcc gctgcggctg
     4381 tcaggtacat ttcaccgcag aggcgcagag gcgcagaggt cttctcgaaa tggtccgagt
     4441 tctctgggag gagtacaatc ggcccgtgcc ccagaccaga ccccggccgt cgcgtgccga
     4501 gcctcgcgaa gtcgtcgaac tgcgccagct gaaggaacgc cagcccgagc tggcgccggc
     4561 cgtcgacctg caggtcgcgc tgatcgagct gcagcggcgg gtgcaggcgc gcgtgccggt
     4621 gccctggatc gaattcgctc ccgactggct gaaggcgcag caggcggcgg ggcgcccgct
     4681 gctccggttc gaggagatcc cgctcgactg gaccgacgtc cggctcgtgt tccgccagac
     4741 ggccgacgtg ctgcgccgct tcgaggcgct cgagccggcc gactaccggg cgttgcaggc
     4801 gctcgggcgg gacggccact cgctcgagcc ctttgtggcg gactggttca atagggccgc
     4861 cgaccccgct cgcgccggtt cgcctccgga cctcccggtt gcggtgagcc ccgaggcgct
     4921 cgagcaggtg ctggcgctga cgctgcgccc gttcctcgag cggtgcgccg aggccgtgcg
     4981 ccagcgggcc gacttcagcg cctggcacct ggcctcgtgc ccgctgtgcg ggggcgagcc
     5041 ggagttggcc acgctcgacc cgcagggcga gcgctgcctc gtgtgcggcc gctgcaccac
     5101 cgaatggcgg ttcgaacgcg gcgtctgccc cttctgccgc aacgacgacc gcagcctgct
     5161 gccctcgttc gggagccggg acggcctgta tcgcctggag gcctgcgacg tgtgccgccg
     5221 ctacctcaag accttcgacg tccgcgccgg ccggcgccac gtcctgctgg cggtcgattc
     5281 ggttgcgacc ctgcccctcg acgccgcggc gatccaacgg ggttacagag gttagaggcg
     5341 ttggctggct gggcggtggg gtgaggctcg aaacacgaag ggcgcgattc ggccgctgcc
     5401 gctcgcgccc cggattccga atcctgaatc ccgaatcctg aatcctgatt actccggctt
     5461 ctcgtccttc ccggccagga gttccttggc cttgccggac acccacccga gcaggttgcg
     5521 cgcctcggtg aagcgcccgg cgttcgagcg cgctcccagc acgaccaccg ccacgggctg
     5581 gttcacctcg ggaagtttca ggagggtgac caggcagtag cccgcgcggt tgatgaagcc
     5641 ggtcttgccg ccgagcacgt tcggctgcag ctcgctgttg gcaagcagcc ggttcgtgct
     5701 gtgaatggtc cgcgcacgct tgccgagcac cagctggtgc tccttcttcc gcatgatctc
     5761 cgagatccac tcgtcgccgg cggcgtaggc gatgagccgc gccatgtcgt aggccgacga
     5821 gacgttcgcc gcatccagcc ccgaggggtc ggcgtaggcg gtgctctgca gccccagctc
     5881 gaccgccttc tcgttcatcc ggcgcacgaa gccgtccgcc ccccacggcg acacgcgggc
     5941 aagtgcccgg gccgcggcgt tgtcgctgga gatcagcagc aggtgcatca ggtcgtccac
     6001 cgagacgacg tagttggcgc gcaggtgcgt ggtcgaggcg gcgtagacgt cggcgcgctc
     6061 gatggtgacc atccgggcgg ggtcggggtc ggtctccgag atgatgaccg ctgtcatcac
     6121 cttggtgatg ctcgcgatcg accgcttgtc ctgggagtgc tcctcccaca ggacctgctt
     6181 ggtctccggg ttgtagatga tggcggccgc ggcgtgcagg tcggggacga gagtcccggt
     6241 cgcatcggtc ttgtaacggg gaagctgcgc ttctcggatg gcggcagcgc gggcgcgcgc
     6301 cagcctggcg cggcgggcgg cggcagcctt cgatcgggca gttcttcggc tcttcgcacg
     6361 gcggaccgta gaagtcttct tggtggtcga cttttgtacg gacgtcccgc gactctgcgc
     6421 ggccgccgcc actgacgcgg gcaggctgac cgagagcccg aaagacacgc acaaggcggc
     6481 tgccagggca gcccacgtgg gatgactacg cagtgacatc ggttgcttca acgaccgggg
     6541 caggacaaga caccggcatt gtagcagaat gctcagccgg ggcaagccct ttctcattca
     6601 ctccatggga cttgcgggct agtccgcctg cgcggcgcct tcgtgctcca tcatggagac
     6661 atgcaaggga cggtccagct gagcttgcgc gccgaagccg catgcgccga atgcaggtga
     6721 aatcggcgca tttcagaagc gtaagcggct gacgaatccg aaccctcccc gtcgtctcag
     6781 tctcgcagca gctcctcgag gccttccgga tcgacgccaa ccactaccct cccatcaacg
     6841 tccagggccg ggacggccgg ggttcccgag aagatcatcg tgcggtggaa cgccagcgga
     6901 ttggtttgaa cgtcgatctc gtcgaaggcg atcccccggc cgcgcaggaa ctggcgaacc
     6961 tcctcgcagc ggggacaacc cgggacggcg tagagcgtca cgtggggcac ggggcctcct
     7021 tggaaaggat ggatgccgga ctggcggcga cgcctgtccc actgcccact agccactgcc
     7081 cactggccgc tgcccgccgg cggtcatctg gccagcgcga acacgagcgc ggtcacccgc
     7141 gaggtcccct tccaggaatc ggtgctgtcg aagctttcgg cgagtgtgtg ggcgccggtg
     7201 gagcggccgc cgccgccaac cgtgatggcc gggatcccca gggcgatcgg gatgttcgcg
     7261 tcggtcgagc cctcgccaag tcgggccggc aggcccagcg cccggttcac cgccagggcg
     7321 gacttgacga tcgaagaatc ctcgggggtc cggcccgccg ggcgatttcc gaccggctcg
     7381 acctgcaccc cgacgcggcc cgtctcccac cgctcgtttt cctggcgaac cgcctgctcc
     7441 acgacgccct tgaaggtcgc gtcgatcgag gcgagcgccg cgccgtcgga agagcgcatg
     7501 tcgatctcga accaggcctc ctcggagatg gagttgaccg aggtgccgcc gccgatgcgg
     7561 ccgacgttga aggtcaccct cggcgaccgc ggcacctcga agtcggccac ccgcgccacc
     7621 gcccgcccca gcgcgtgcac cgggttgacg gtgccgaacg cgccgaagct gtgccccccg
     7681 ggccccttga aggtgacgcg ataccggcgg ctgccaaccg ccacgttcgt gatcccatga
     7741 ccgcaaccgt cgatcgagac gaaggcgtcc acgcgtccct tcagctcgtg gccgatgagg
     7801 tgtctcactc ctcgcaggtc gcccagcccc tcctcgccga cgttggcgac gaaggtgatc
     7861 gtccccgggg tccgcacccc gccgcggtcg agagaccgcg cgacccccgc cagtaccgcc
     7921 agcccccggc catcgtcggc aatgccgggc ccgcgcagga tgggaccttc cctggtcacc
     7981 ttcacgcggg ttccctcggg gaagaccgtg tcgaggtgtg cggccaccac gaggttcggc
     8041 cgcgcggcca ggccgggacg ctcgccgatc acgttgccgg ccgcgtcaat ccggacgttc
     8101 cgcagcttca gcgcttcgaa cacctgtttc aggcgggcag cgcggacgcg ctcggcgaag
     8161 ggcggcgccg ggatctcggt gaacgcaacc tggtcgtcga gcagcgccgg ttcggctgcc
     8221 tccacgccgg ccagcgccgc ctgcagggac ggatcccgca tcaaggccgc gaccgcgtcc
     8281 tcgggggctc ccttcgcccc gccgggtccg ccggtgaccg cgcccgcggc cgcgagcgcc
     8341 atcgcgatgg cgcagagaat ggcaacccgg cgccacatcc tgtcacttcc tgggcaggcg
     8401 ttcatgcggc caccaaggta tacggaatgc ccggccacct caagcgccga tccaaccggt
     8461 tggccggcgg atccagcggg ctgaagcatt ccggatccac cgtggcagcg gggcgaggaa
     8521 cgctcgtgcc gaattccggc ttctgcgacg ctggaatcgt cgccactgca accccagccg
     8581 cggcacgccg attgcaccca gctcggctgg agcttgatat gagcctcgtt cgcctcctcg
     8641 gcttcgccac cctccagtgc ggctgcgtcg tcggccggta tctcgagctc tcgagttccc
     8701 gcgaggtggt gtacgtggaa gagaaggggg tggagtgcgc gatgcaccac caccgccgga
     8761 atcatgccct tccccgccgc ttctccggtg ccgtcccggt tgtggtcgtc gccaaagctt
     8821 cgtagaatcc ggggtggtga ctgcccggcc cacgccgtac gcctcgctgc gccaccccaa
     8881 cggcagctct ctcgacttct ttctcggtga ctgcctcgag ctgctgccct gtcttgcctc
     8941 tgaacgggtc gacgtggtcg tcacctcgcc gccctacaac ctgggcgtga agtatcggtc
     9001 ctaccaggac accgtaccgc gcgaccggta cctcgagtgg acgggcgagt gggtgcaggc
     9061 cgtgcgggcg atcatgggcc ctggcgcctc gctcttcctg aacgtgggcg ccaagccgaa
     9121 ggacccgtgg gtggccatgg atatcgccca ggccgtgcgg cctcacctgc ggctgcagaa
     9181 cacggtgctc tgggtgaagt cgatcgtgat cgagcgggcc gcggcggggg aggacgcgat
     9241 gcccctggtc ccgaccggtc tgcccgccgg gcggttggac ggcgccgtcc gggcggcgct
     9301 gctgtgtcgg ctcccgaccg tgccgttcct ccgaccggcc gg
//
LOCUS       DAOPQX010000121         9203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8475_length_9203_cov_4.586139, whole genome shotgun sequence.
ACCESSION   DAOPQX010000121 DAOPQX010000000
VERSION     DAOPQX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8475_length_9203_cov_4.586139"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKK95_08190"
     CDS             complement(<1..489)
                     /locus_tag="PKK95_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HOC18231.1"
                     /translation="MGHDLLTIYFLKGIEYLLAVAYLPLFVLFWRFATPKAPAVAVAA
                     VGDKAPGWADQLADYFRLPGDLFHHPGHAWARVEGDTVVVGLNDFAQRLVGRIDRLRL
                     PAAGTELAQSRPAFSIVSGGRSVAVLAPVGGTVVEVNHEAASDPGAVKQSPYGRGWLL
                     RVR"
     gene            complement(516..1745)
                     /gene="nrfD"
                     /locus_tag="PKK95_08195"
     CDS             complement(516..1745)
                     /gene="nrfD"
                     /locus_tag="PKK95_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOC18232.1"
                     /translation="MTDTTFDRGLGPREWVIEKVFLGMPLGAYLRSLVTPGNLLVALI
                     LIPGIPAVVYRFVYGIGAVSNLTQANPWGLWIGLDVMSGVALAAGGFTIATLVHVLGL
                     RDYQPIVRPAILTGFLGYLFVVIGLMADLGRPWNLPVPMVYSFGTGSVMFEVGWCVAL
                     YLTVLALEFSPAVLEWLGLRKMREWALKLTLALTVLGLCLSTLHQSSLGALFLMMPHR
                     VHPLWYSGFVPIFFFVSAIAAGIAMVIVESALSHRAFGSRVAPGSHERLDRITLGLAR
                     AGALVLFAYFFLRLQGLVDSGRFDLLNTGYGYWFLFELLGFVLVPAILFGLASRQRRV
                     VLARWVAGWTVIGIVVNRLNLSVITFNWNSPERYVPSAMEVLISVTIITLGVMTFRWI
                     VNRMPVLRDDPAFPPAH"
     gene            complement(1738..2637)
                     /locus_tag="PKK95_08200"
     CDS             complement(1738..2637)
                     /locus_tag="PKK95_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC18233.1"
                     /translation="MDVSRRTFFKIAGVAGAAGIAGQPAKAEAASLAAGHSPAVLVDA
                     TRCIGCRGCEAACAEANGLPGPADAGGEAVFEARRKTSPGAYTVVNRYAHAQAEGGYR
                     FVKTQCMHCADPACASACLAKALEKTAAGPVVYHPDRCLGCRYCMVACQFDVPKFEFE
                     KAAPFVSKCSFCAERQAQGQPPACASVCPTGALQFGQRGELLEEARTRIYQNPGTYVR
                     HIYGEHEAGGTNWLYISDVPFDQLGFRTDLGPTSPPELTRTALAAVPVVLTLWPPLLM
                     GLYTWSQRLKGASRQGGQEASHD"
     gene            complement(2735..2929)
                     /locus_tag="PKK95_08205"
     CDS             complement(2735..2929)
                     /locus_tag="PKK95_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18234.1"
                     /translation="MLFCAAYPVKKMVPLDRLASANPCLGGDFQGCPLFRELSERLKA
                     CPEQECAVIPPPEPSKGGRS"
     gene            complement(3239..4624)
                     /locus_tag="PKK95_08210"
     CDS             complement(3239..4624)
                     /locus_tag="PKK95_08210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC18235.1"
                     /translation="MSRERTRILIVDDEEIVRESLSAWLEKDGYTLATAPDGETALER
                     IRGERWSILLVDLKMPGIDGLQVLEQARTLQPEAASVIMTAYATVDTAVEAMKLGAYD
                     YLVKPFDPEELSLMIQKIVAQQALVRENVLLRKVLKREYRFRDLISKSPAMQAVFDLA
                     RTAARSSSTILILGESGTGKELLARAVHAESPRSQGPFVAVSCAALTETLLESELFGH
                     EKGAFTGAVARRKGKFEMAQGGTLFLDEIGDISPKLQLDLLRVLEDRRFFRVGGSESV
                     GVDARIIAATNKDLQKAVAEGTFREDLFYRLNVIPVHLPPLRDRIVDLPLLVEHFLEQ
                     LGAEMNRHVDEVSSEAMAALMAHSWPGNVRELRNVLERAIVVASGRVIQVADLGLRRP
                     SAEGAGVGPLASLDEVEKHHIAVVLADCGGNISQAARTLGIDRATLYNKMKKYQLRKD
                     GEPEPEEHLTT"
     gene            complement(4617..5174)
                     /locus_tag="PKK95_08215"
     CDS             complement(4617..5174)
                     /locus_tag="PKK95_08215"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HOC18236.1"
                     /translation="MVDVFLWWIGAGVADEDLLTTLRAEVTRVFEVGARCWHGHERPL
                     DSFDARRQQHSSTRILRWLLEARPRHSGKIVGLTDVDLFIPILTFVYGEAQLGGRAAV
                     VSTARLASDNGFRLDRALLRERTMKEAIHEIGHTFGLIHCERRTCVMSRSVNLVQVDA
                     KDHGLCPDCRRRYAELREQGHHEHE"
     gene            complement(5185..6828)
                     /locus_tag="PKK95_08220"
     CDS             complement(5185..6828)
                     /locus_tag="PKK95_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC18237.1"
                     /translation="MDSQEPRVLPAAAPPDVRLRWWQRLGVKVVLVSAVVTFGLVALF
                     VGFTLRQQQRYMTQEVLRGAAQFSDTLKAATYHFMLADERESAYRAMRTVGGLDGIHR
                     VRMLNKEGRVTFSTDDRETGRMVDKGAEACYACHQQGQPLVRLNTPSRSRIFEQDGQR
                     VLAMITPIYNEASCSTASCHAHSPDQQVLGVMDVSMSLREEDAAVASLRRSTLLLAGL
                     AVLALAAFGALFLRWYVTRPLADLVAATGRIAEGDLDLSVAVRRTDEIGVLAGSFNTM
                     TSSLREARAELQRLNEGLERQVAERTSALRDAQAQLVQSEKMSSLGKLAASVAHEINN
                     PLSGILTYAKLLIRLHEEGELTAQAREACTRNLRLVQRETERCSAIVRNLLDFARQRP
                     PALKDLDLSAVAEEALSLLAHRLQMQNVTLVKDLASLPTVRADFGQMRQAFVNIALNA
                     AEAMAEGGTLTVRSRVEGGMAAVEFADSGPGIPPEHLSRILDPFFTTKEKGTGLGLSV
                     VYGLIERHSGKLDISSRVGQGTTVVIRLPIAGAGTEAAV"
     gene            7137..7514
                     /locus_tag="PKK95_08225"
     CDS             7137..7514
                     /locus_tag="PKK95_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18238.1"
                     /translation="MTKRMGGTAAKGGFYWNLGKWELTMIPKQGGILPGTVSDRYVRV
                     PVVGLLVLAPMMGAVYAFFLPFIGFAMLFGFMAKKAFGVTRAGAAGVAATIAPSWRPG
                     EAYLAGKKKDKGEGQDAETGGRQ"
     gene            complement(7518..8177)
                     /locus_tag="PKK95_08230"
     CDS             complement(7518..8177)
                     /locus_tag="PKK95_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC18239.1"
                     /translation="MARIRVLIADDHAIVREGVRALLTLSDDIEVAGEAANGQEAVEL
                     ARRLRPDVILMDIAMPGLGGLEATLAIRRENPDAKILVLTQYEDREYIRRFLKAGVSG
                     YVLKKAAGSELTAAIRAVQRGGLVLDPEVAREAWRDQAAAAAPVEVADPYETLTDREK
                     QVLKLVAEGRSNKEVAELLEISVKTAMSHRERIMRKLDLHSRTELIRFALQKGVIRVD
                     G"
     gene            complement(8146..>9203)
                     /locus_tag="PKK95_08235"
     CDS             complement(8146..>9203)
                     /locus_tag="PKK95_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC18240.1"
                     /translation="RQDQAETFAEARRLRRAMLWLGPTLLALALVFAWGAARSVRRPL
                     GLLTLAAERIASGDLEQPIPRLPEDEVGRLGRSLEGMRVALKGSLDEIASANLALEGR
                     VEARTRELQGLYRELQERERWREELLRKVISAQEDERKRLARELHDETSQTLSALAMK
                     IETALAASPPGAPRERLVEARALTVRTLDELHRLIFDLRPSVLDDLGLLSAIRWYAER
                     HLEPLGIIVQFEASGFERRLRPELETALFRVAQEAITNIAKHAGAETVLIQCLERDGR
                     VTIEIEDDGKGFAPESLPPPAARERGLGLMGMRERVELFGGTIELDSAPGQGTRVKVS
                     VKVREVVDGQDSGADRR"
BASE COUNT         1419 a         3245 c         3196 g         1343 t
ORIGIN      
        1 gcgtaccctc agcagccagc cccggccgta gggcgactgc ttcaccgcgc ccggatcgga
       61 tgccgcctcg tggttgacct cgaccacggt gccgcccacc ggcgcgagca ccgcgaccga
      121 gcgcccgccc gacacgatcg aaaacgccgg gcggctctgc gccagctcgg tgcccgcggc
      181 gggcaggcgc aggcggtcga ttcgcccgac caggcgctgc gcgaaatcat tgaggccgac
      241 caccaccgta tcgccctcca cccgcgccca ggcgtgcccg ggatggtgga agaggtcacc
      301 gggcagacga aagtagtccg cgagctggtc ggcccacccg ggcgccttat cgccaaccgc
      361 cgccacggcc acggccggcg ccttcggcgt cgcgaatcgc cagaagagga cgaagagcgg
      421 caggtacgcc accgccagca ggtattcgat ccccttgagg aaatagatgg tcagcaggtc
      481 gtgtcccatg gtccgtgtgc actccgtacc cgccgctaat gcgccggcgg aaacgccggg
      541 tcgtcgcgga gcaccggcat ccggttgacg atccagcgga aggtcatcac cccgagggtg
      601 atgatcgtga ccgagatgag gacttccatc gcgctcggga cgtagcgctc gggcgagttc
      661 cagttgaacg tgatcaccga gaggttcaac cgattcacca cgatcccgat caccgtccag
      721 ccggccaccc agcgagccag caccacccgc cgctgccggg aagccagccc gaacaggatg
      781 gcggggacga gaacgaaccc gagcagctcg aacaggaacc agtagccgta gcctgtgttc
      841 aacaggtcga accggccgct gtccaccagc ccctggagcc gcaggaagaa gtaggcgaag
      901 agcaccagcg cccccgcccg cgcgagaccg agcgtgatgc gatcgagacg ctcgtgcgag
      961 cccggcgcaa cccggcttcc gaacgcccgg tgcgacaggg cgctctcgac gatcaccatc
     1021 gcgatcccgg cggcgatggc cgagacgaag aagaagatcg gtacgaaccc cgagtaccac
     1081 agcgggtgca cccggtgcgg catcatcagg aagagggcgc cgagcgacga ctggtgcagc
     1141 gtggagagac agaggcccag gacggtcagc gcgagcgtga gcttcaacgc ccactcgcgc
     1201 atcttccgca ggccgagcca ctcgaggacg gccgggctga actcgagcgc gagcaccgtc
     1261 aggtacagcg ccacgcacca gcccacctcg aacatcaccg acccggtgcc gaacgagtag
     1321 accatcggga ccggcaggtt ccacggccgg ccgagatcgg ccatcaggcc gatgacgacg
     1381 aacaggtagc ccaggaagcc ggtgaggatc gcgggccgga cgatcggctg gtagtcgcgc
     1441 aggccgagca cgtgcacgag cgtcgcgatg gtgaagccgc cggcggccag cgcgaccccg
     1501 ctcatcacgt cgaggccgat ccagagcccc caggggttgg cctgcgtcag gttgctcacg
     1561 gcgccgatgc cgtagacgaa gcggtagacg acggccggga tgccggggat caggatcagc
     1621 gccaccagca ggttgccggg cgtgaccagc gaccgcaggt aggcgccgag gggcatgccg
     1681 aggaagacct tctcgatgac ccactcgcgg ggcccgaggc cgcggtcgaa ggtggtgtca
     1741 gtcatggctg gcctcctggc caccctgccg ggaagcccct ttgagacgct ggctccaggt
     1801 gtagagcccc atcagcagcg gcggccagag cgtcagcacg acgggtacag ccgccagggc
     1861 ggtgcgcgtc agctcgggcg gagaggtcgg cccgaggtcg gtgcggaagc ccaactgatc
     1921 gaacggcacg tcgctgatgt agagccagtt ggtcccgccc gcctcgtgct cgccgtagat
     1981 gtgacgcacg taggtgcccg ggttctggta gatgcgcgtc cgcgcctcct cgagcagttc
     2041 gccgcgctgc ccgaactgca gcgcgccggt ggggcagacc gacgcgcacg ccggcggctg
     2101 cccctgggcc tgccgctcgg cgcagaacga gcacttgctc acgaagggcg ccgccttttc
     2161 gaactcgaac ttcggcacgt cgaactggca cgccaccatg cagtagcggc acccgaggca
     2221 gcggtccggg tggtagacca cggggccggc cgccgtcttc tcgagcgcct tagcgaggca
     2281 ggccgaggcg cacgcgggat cggcgcagtg catgcactgc gtcttcacga accggtagcc
     2341 tccctccgcc tgcgcgtggg catagcggtt gacgaccgtg taggcgccgg ggctggtctt
     2401 gcgccgcgcc tcgaacaccg cctcgccgcc ggcgtcggcc ggcccgggca ggccgttcgc
     2461 ctcggcacag gcagcctcgc agccgcggca gccgatgcac cgcgtggcgt ccacgagcac
     2521 ggcgggggag tggccggcgg ccagcgaggc tgcctcggcc ttcgccggct gaccggcgat
     2581 tcccgccgcg ccggccacgc cggcgatctt gaagaacgtt ctccgactta cgtccattgg
     2641 gtctctcccc tccgtcgggc tcaccggagg tgagcggcca gcgaggaaaa gggcaaaatg
     2701 cacataccga gcagcgccag ggcgagggcg aacatcacga cctgcctccc ttcgatggtt
     2761 ccgggggcgg gatgaccgcg cactcctgct cggggcacgc cttcagccgc tcggacagtt
     2821 cccggaacag cgggcaaccc tggaagtcgc ccccgaggca cgggttggcg gacgcgagcc
     2881 ggtcgagcgg caccatcttc ttgaccgggt acgcggcgca gaacagcatc acggcttctt
     2941 tgaggtgtgg gcaggacatc ggtatcaggt ctcaccccac gtcatcgcaa gcgtgatgcc
     3001 acctcggtcg tcgtcctgta acgcttgtga aatcaatcac ttattcgatg tgcggacgcg
     3061 cacggcccaa tttgaagccg aaggcctgtt gaaatcgtcc acaccgggcg aagccgcgtt
     3121 gggcggatcg gcccgtgggc cgctcgtttt gcttggattt tccgcgaaag aggggaggtg
     3181 ttgaaaacga cagcggcctg ttgcgggatt caacagaccg ctgtcgccga gagcggaact
     3241 acgtggtcag gtgctcctcg ggctccggct cgccgtcctt gcgcagctgg tacttcttca
     3301 tcttgttgta cagcgtcgcg cggtcgatgc cgagcgtgcg ggcggcctgg ctgatgttgc
     3361 cgccgcagtc ggccagcacc acggcgatgt ggtgcttctc gacctcgtcg agcgaggcga
     3421 gcgggccgac gccggcccct tcggccgatg ggcggcgcag gccgaggtcg gcaacctgga
     3481 tcacgcggcc cgacgcgacg acgatggcgc gctcgagcac gttgcgaagc tcgcggacgt
     3541 tgccgggcca cgagtgggcc atcagcgccg ccatcgcctc ggacgagacc tcgtcgacgt
     3601 ggcggttcat ctcggcgccg agctgctcga ggaagtgctc gacgagcagc gggaggtcca
     3661 cgattcgatc gcgcaagggc ggcaggtgca ccgggatgac gttgaggcgg tagaagaggt
     3721 cctcgcggaa ggttccctcg gcgaccgcct tctgcaggtc cttgttggtg gcggcgatga
     3781 tgcgggcgtc gacgccgacc gactcggagc cgccgacccg gaagaagcgg cggtcctcga
     3841 gcacgcgcag caggtcgagc tgcagcttcg ggctgatgtc gccgatctcg tcgaggaaca
     3901 gggtgccgcc ctgggccatc tcgaacttcc ccttgcggcg ggcgacggcg ccggtgaagg
     3961 cgcccttctc gtggccgaag agctcggact cgagcagcgt ctcggtgagc gcggcgcacg
     4021 agaccgcgac gaagggcccc tggctgcgag ggctctcggc gtggacggcg cgggcgagca
     4081 gttccttgcc ggtcccgctc tcgccgagga tcaggatggt ggaggagctg cgcgcggcgg
     4141 tgcgcgccag gtcgaacacc gcctgcatgg cggggctctt gctgatgagg tcgcggaacc
     4201 ggtactcgcg cttgagcacc ttgcgcagca gcacgttctc ccgcacgagg gcctgctggg
     4261 cgacgatctt ctggatcatc aggctcagct cctcgggatc gaacggcttc accaggtagt
     4321 cgtaggcgcc cagcttcatc gcctcgaccg cggtgtcgac cgtcgcgtac gcggtcatga
     4381 tcaccgaggc ggcctcgggc tgcagcgtcc gcgcctgctc gagcacctgc agcccgtcga
     4441 tgccgggcat cttcaggtcg accagcagga tcgaccagcg ctcgccgcgg atccgctcga
     4501 gggcggtctc gccgtcgggg gccgtcgcca gcgtgtagcc gtccttctcg agccaggccg
     4561 acagcgactc gcgcacgatc tcctcgtcgt cgacgatcag gatgcgcgtc cgttctctac
     4621 tcatgctcgt ggtgtccttg ttcccgcaat tccgcgtagc gccggcggca gtcggggcag
     4681 aggccgtggt ccttggcgtc gacctgcacg aggttcaccg accgcgacat gacgcaggtt
     4741 cgccgctcgc agtggatgag cccgaaggtg tggccgatct cgtggatcgc ctccttcatg
     4801 gtccgctcgc gcagcagggc gcgatcgagg cggaagccgt tgtcggaggc cagccgcgcg
     4861 gtcgagacga cggccgcacg cccgccgagc tgcgcctcgc cgtagacgaa ggtcaggatc
     4921 gggatgaaca ggtcgacgtc ggtgaggccg acgatcttgc ccgagtggcg tgggcgcgcc
     4981 tcgagcagcc accgcaggat gcgcgtcgag gagtgctgct gccgccgggc gtcgaacgag
     5041 tcgagcggcc gctcgtggcc gtgccagcag cgcgcgccca cctcgaagac gcgcgtcacc
     5101 tcggcccgca gcgtcgtcag caggtcctcg tccgccaccc ccgcaccgat ccaccagaga
     5161 aagacgtcga ccacgcgccc tcgtctagac ggccgcctcc gtacccgcac cagcgatcgg
     5221 cagccggatc accaccgtcg tcccctggcc cacccggctc gagatatcca gcttccccga
     5281 gtgccgctcg atcaggccgt acaccaccga cagccccagg cccgtgccct tctccttggt
     5341 ggtgaagaac ggatcgagga tgcgcgagag gtgctcgggg gggatgcccg gcccgctgtc
     5401 ggcgaactcg acggccgcca tgccgccctc gacgcgcgac cgcaccgtca gcgtgccccc
     5461 ttcggccatc gcctcggccg cgttgagcgc gatgttgacg aacgcctgcc gcatctggcc
     5521 gaagtcggcg cgcacggtgg gcagggacgc caggtccttc accagcgtca cgttctgcat
     5581 ctgaagccgg tgcgcgagca gcgagagcgc ctcctcggcg accgcgctca ggtcgaggtc
     5641 cttgagcgcc ggcgggcgct ggcgggcgaa gtcgagcagg ttgcggacga tcgcgctgca
     5701 gcgctcggtc tcgcgctgca ccaggcgcag gttccgcgtg cacgcctcgc gcgcctgcgc
     5761 ggtcagctct ccctcctcgt gcagccggat cagcagcttc gcgtaggtga ggatgcccga
     5821 cagcgggttg ttgatctcgt gcgcgaccga ggccgccagc ttgccgagcg acgacatctt
     5881 ctccgactgc accagctgcg cctgcgcgtc gcgcagcgcc gaggtccgct cggccacctg
     5941 gcgctcgaga ccctcgttca gccgctgcag ctcggcgcgc gcctcccgga gcgacgaggt
     6001 catggtgttg aacgagccgg ccaggacccc gatctcgtcg gtgcgccgca ccgcgacgct
     6061 caggtccagg tccccctcgg cgatccgccc cgtcgccgcc accaggtcgg cgagcggacg
     6121 cgtcacgtac caacgcagga acagcgcgcc gaaagccgcc agcgccagca cggccagccc
     6181 cgccagcagc agcgtcgacc ggcgcagcga cgccaccgcc gcgtcctcct cgcggagcga
     6241 catcgacacg tccatcaccc cgagcacctg ctggtcgggg gagtgcgcgt ggcacgaggc
     6301 ggtcgagcag ctcgcctcgt tgtagatggg cgtgatcatc gccagcacgc gctggccgtc
     6361 ctgctcgaag atgcggctgc gcgagggcgt gttgaggcga accagcggct gcccctgctg
     6421 gtggcacgcg tagcaggcct cggccccctt gtccaccatc cgcccggtct cgcgatcgtc
     6481 ggtggagaag gtgacgcgcc cctccttgtt cagcatccgc accctgtgga tgccgtcgag
     6541 gccccccacc gtccgcatcg cccggtacgc gctctcccgc tcgtcggcga gcatgaagtg
     6601 gtaggtagcg gccttgagcg tgtcgctgaa ctgcgccgcg ccgcgcagca cctcctgggt
     6661 catgtaccgc tgctgctgcc ggagggtgaa gccgacgaac aacgccacca ggccgaaggt
     6721 gaccacggcg ctgacgagca ccaccttcac ccccagcctc tgccaccacc ggaggcggac
     6781 gtcgggcggc gccgcggcgg gcaagacgcg cggttcctgg gaatccatgg cgaaagccga
     6841 ccgccccgtg agcggggcat cgaggggggc cacgcgcccg gcgacgcgca gtcctattct
     6901 acaggtcctt ttcggcttct ggaagtccgg gtgttgagaa agtcagcggc cggcgtttcc
     6961 gttcaggctg tcgtgaacga tgccggctag ggtatttttc cccccgtccc tcgggtgtat
     7021 tcccgcgccg tggccgtcgg tcgcctgatt gtggctcccc aggggcggcg ctacagtagt
     7081 cccaggatga aacgaacgcc ggcgcgcggt gagggccggt tcagcgaggt ggcatcatga
     7141 cgaagagaat gggcggcacg gcggcgaagg gcggcttcta ctggaacctc ggcaagtggg
     7201 agctgacgat gatcccgaag caggggggaa tcctgccggg caccgtgtcc gatcggtacg
     7261 tcagggtgcc ggttgtcggc ctcctcgtgc tcgccccgat gatgggggcg gtgtacgcct
     7321 tcttcctccc gttcatcggc ttcgcgatgc tcttcggctt catggcgaag aaggcgttcg
     7381 gcgtcacccg ggcgggcgcg gccggcgtgg cggccacgat cgccccgagc tggcgccctg
     7441 gcgaggcgta cctggccggg aagaagaagg acaagggtga gggccaggac gcggagaccg
     7501 ggggacggca gtaagcgtca gccgtccacc cggatcacgc ccttctgcag cgcgaagcgg
     7561 atcaactcgg tgcggctgtg gagatcgagc ttccgcatga tccgctcgcg gtgcgacatg
     7621 gcggtcttca cgctgatctc gagcagctcg gccacctcct tgttgctgcg cccctcggcg
     7681 accagcttga gcacctgctt ctcgcggtcg gtcagcgtct cgtaggggtc ggccacttcc
     7741 actggcgcgg ccgcggctgc ctggtcgcgc cacgcctcgc gcgccacctc ggggtcgagg
     7801 acgaggccgc cacgctgcac ggcgcggatg gcagcggtca gctccgagcc cgcggccttc
     7861 ttcagcacgt agcccgacac gcccgccttc aggaagcgcc ggatgtactc ccggtcctcg
     7921 tactgcgtga gcacgaggat cttcgcgtcg gggttctcgc gccggatggc gagcgtcgcc
     7981 tcgaggccgc cgaggccggg catggcgatg tccatcagga tgacgtcggg gcgcaggcgg
     8041 cgcgccagct ccacggcctc ctgtccgttg gcggcctcgc cggccacctc gatgtcgtcc
     8101 gacagggtga ggagcgccct cacaccctcg cgcacgatcg cgtgatcatc ggcgatcagc
     8161 acccgaatcc tggccatcga cgacctcccg caccttcacg ctgaccttca cccgcgtccc
     8221 ctggccgggc gcggaatcga gctcgatcgt tcctccgaac agctcgacgc gctcgcgcat
     8281 ccccatcagc cccagccccc gctcgcgcgc cgccgggggc ggcaggctct cgggcgcgaa
     8341 gcccttcccg tcgtcctcga tctcgatggt cacccggccg tcgcgctcga ggcactggat
     8401 gagcaccgtc tcggcgccgg cgtgcttcgc gatgttggtg atggcctcct gcgcgacgcg
     8461 gaacagggcg gtctcgagct cggggcggag gcggcgctcg aagccgctcg cctcgaactg
     8521 gacgatgatc cccagcggct cgaggtggcg ctcggcgtac cagcggatgg ccgacagcag
     8581 gccgaggtcg tcgagcaccg aggggcgcag gtcgaagatc agccggtgca gctcgtccag
     8641 cgtgcgcacc gtcagcgcgc gcgcctcgac caggcgctcg cgcggggcgc ccggcggcga
     8701 ggcggccagc gcggtctcga tcttcatcgc gagcgcgctg agcgtctggc tggtctcgtc
     8761 gtgcagctcg cgcgccaggc gcttgcgctc gtcctcctgc gccgagatca ccttccgcag
     8821 cagctcctcg cgccagcgct cgcgctcctg cagctcgcgg tagaggccct gcagctcccg
     8881 cgtgcgcgcc tcgacccgcc cctcgagggc gaggttggcc gaggcgatct cgtcgagcga
     8941 gcccttgagc gccacgcgca tcccctcgag cgagcgaccg agccggccga cctcgtcctc
     9001 cggaagccgc ggaatcggct gctcgagatc gcctgaggcg atccgctcgg ccgcgagcgt
     9061 cagcaggccg aggggccggc ggacgctccg cgccgcgccc cacgcgaaga ccagcgccag
     9121 cgcgagcagc gtcgggccca gccacagcat cgcccggcga agccgcctcg cctcggcgaa
     9181 ggtctcggcc tggtcctgcc gca
//
LOCUS       DAOPQX010000122         9155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8514_length_9155_cov_4.382308, whole genome shotgun sequence.
ACCESSION   DAOPQX010000122 DAOPQX010000000
VERSION     DAOPQX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8514_length_9155_cov_4.382308"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2436)
                     /locus_tag="PKK95_08240"
     CDS             complement(<1..2436)
                     /locus_tag="PKK95_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOC18241.1"
                     /translation="MAFTRPDIRRIRRGLRRTYDNLGPQRGLISAAFVLVVLLVGAAS
                     VQAFRFAAGVLTGLPGRSELQQMTRMAQATTIFDRAGEPAFSLSKEQRIEVRLDDVSP
                     LVLQALIAVEDQRFYRHRGYDPWRLAAAMWANVRARRAAQGASTITQQLARQWFLTPD
                     KTLRRKLREIVLANRIEQLFSKREILELYLNKVYFGDGLYGIEAAARGYFGRRASELT
                     LSQAALLAGLVQSPSAYAPTVNLQRALARRNVVLQAMLDTGAIDRDEHARARAETVAL
                     NDGLRREEPWGRYFKEQVRRELVSRFGLEQVYEGGLRVYTTIDPSMQQHAEAAVKETL
                     DLLEARRRKESRRRPDAAGPEEEPLEAALVAIDPATGEVRAMVGGRDFELSRFNRAMQ
                     ARRQPGSAFKPLVYAAALESGYTPASLLDGLNDPVLTPEGEWVPEDEHLESSTMTIRT
                     ALRTSSNRAAVRMLQTVGIGRTVDYAERMGLGTVPSVPSLALGSGEVTLLSLSSSYVP
                     FANGGLARPPLLVTKVEDRDGKVLYEAARLGTRVVSEQTAFLMSTMLADVINGGTAYR
                     ARQMGFRLPAAGKTGTTNDYQDAWFVGYTPSLLTGVWVGFDRPRPIVRDGYAGDVAVP
                     LWARFMGAATKDDKPRWYSPPSGLTALRVCRISGQLATPGCDHVPVLNDEGQSEERSY
                     SYTEYFVRGTAPSGYCPLHEQRFAFGEATDLPDGARTVAGGAPAPSAWPSGRAEEAGD
                     DRPAEAKATGASEEPKKKKKGFWGRLLGVFKGGDDGKKDDEKGNGDSKKKQDEKKKQD
                     EKKKQDEKKKQEEA"
     gene            2670..3026
                     /locus_tag="PKK95_08245"
     CDS             2670..3026
                     /locus_tag="PKK95_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18242.1"
                     /translation="MRGQLMLRAFTISGGALAIIALFVLGVSGGVQLEPRARTVVPAP
                     SLSEPRIPPPPGASDSAVPMFPAPPRLLEGKTRTTLDGDDINDAIATYGIDDAGNLYE
                     VHSPHTEVPSLGGPRT"
     gene            complement(3143..3706)
                     /locus_tag="PKK95_08250"
     CDS             complement(3143..3706)
                     /locus_tag="PKK95_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOC18243.1"
                     /translation="MAQTQIRIGGFGGQGVILSGIIIGRAAAIHAGLNATLVQSFGPE
                     ARGSSSSAELIVSNEPIWYPYVTNLDMLVVLSQDAYNKFGRALRPGGLLVTEADLVKP
                     EPPLSAARHFVVPATRLAEEIGRKIVLNIITVGFFGAVSQLMPFEALEQAVKDSVPPG
                     MLDLNLEAFRRGWEAGERALNQVPAVV"
     gene            complement(3706..4554)
                     /locus_tag="PKK95_08255"
     CDS             complement(3706..4554)
                     /locus_tag="PKK95_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOC18244.1"
                     /translation="MSEQANPVERYLRSERIPHIWCPGCGIGTTVNCFTRAIDESGIP
                     MDRLAVVSGIGCTGRVAGYMHVDSFHTTHGRAIPFATGLKLAAPGMKVVVYSGDGDLF
                     AIGGNHFIHAARRNLDMTVVCVNNLTYGMTGGQLTPTTPEEAIASTSPYGNFEQPFNL
                     PYLAESCGAVYVARWTTYHVTHLKKAMREALLKKGFSFVEVISPCPTLYGRRNRIGDG
                     LDEVKTYKQRSVIRHGAPTNEVSLGMSGPIVVGKFVDRERPTYQDAMDQFYSRKLGDR
                     YARASQ"
     gene            complement(4551..5705)
                     /locus_tag="PKK95_08260"
     CDS             complement(4551..5705)
                     /locus_tag="PKK95_08260"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOC18245.1"
                     /translation="MHADPAGVLTGAHFLDGDHAACEGALAAECRFVAGYPITPSTEV
                     VERIAQRFPQVGGVFIQMEDELASSIAIAGAVWGGKRAMTVTSGPGFSLMMEHVGFAV
                     MTETPCVFINVQRGGPSTGLPTLPAQADMMQARWGSHGDYEIVALSPNSPQECFDLTF
                     KAFNLAEQYRIPVLVMMDECVGHMMERVVVPRPEELEIYPRKWTKAAPGEYLPYAPGE
                     DLVPAMARAGQGYRFHMTGLTHNERGYPDMSVAAQARLVTRLTDKIKKNADALQDVVE
                     DGVEGADAVVVCYGITSRVALPAIEACRASGLKVGWLRLRTVWPFPERLIRDLASRPH
                     AFVVPELNMGQMVREVERCAGAARVVAVPHAGGTVHDPAVIERAIREAVR"
     gene            complement(5787..6017)
                     /locus_tag="PKK95_08265"
     CDS             complement(5787..6017)
                     /locus_tag="PKK95_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC18246.1"
                     /translation="MAEPTRRVRGVVVIHAERCKGCGLCVEFCPTDVLALSPAFTTKG
                     YHPPEVVRGEACSGCDLCGMYCPDFAIYGYRP"
     gene            complement(6244..7236)
                     /locus_tag="PKK95_08270"
     CDS             complement(6244..7236)
                     /locus_tag="PKK95_08270"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC18247.1"
                     /translation="MQPEVVVVTGASAGVGRATARLFGRRGARVGLLARGRDGLEAAR
                     REVEEAGGTALVLPTDVADASRVERAAERIEGELGPIDVWVNNAMVSVFSPVKEMTPE
                     EYRRVTEVTYLGVVHGTLAALRRMLPRDRGTIVQVGSALAYRGIPLQSAYCAAKHAVQ
                     GFCDSLWSELLHDGSRVHLTMVQLPALNTPQFSWVKSRLPRRPQPVPPIYQPELAAEA
                     ISYASRAGRREIYFGWPTIQAIVGNKLAAWYADRYLARNGYESQQYDGEIDPDRPHNL
                     WDPLPGDHGAHGDFDDRSTGSSPQLWLNMRRDWVVAAAASLALFLAAGRAARAL"
     gene            complement(7242..7505)
                     /locus_tag="PKK95_08275"
     CDS             complement(7242..7505)
                     /locus_tag="PKK95_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18248.1"
                     /translation="MATTNPIRAAEQQTEGPIARAIEEQTAKLPSDTFMWAALGSMAT
                     SLSFELMGKREMALFVGQWAPTLLIFGLYNKLVKQLGHDRLNP"
     gene            complement(7693..>9155)
                     /locus_tag="PKK95_08280"
     CDS             complement(7693..>9155)
                     /locus_tag="PKK95_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC18249.1"
                     /translation="LVAEAGSRATGRVSGFAGVARCGWSAIGAIEAARAGRAPIVAAR
                     ARGPIDVEAGVRHASALGAVTWTSGPAFTGTARVNPFGEDRDNGTPAQRNDTRSVQLA
                     LAASGVAGAGGWEVRAWGQRARFGQTFSTVAPGRGSETPTITQHVDSDAVGGRASWNQ
                     PLGLLAVVAGFEARQVHADNVETPTLGGLSRTAGGRQRGPAVFARMAAPLGRRLSIEG
                     GARWETWANQAAAGGESRRASSLSPRISIAWHASPGVVVNASAYRAFRAPTLNELYRG
                     FRVGSIVTAPNDSLGPERSSGTEAGIMMTRRRLSVRATGWWSRLDDPVANVTIGPGER
                     QRQNVGEIRAYGLETELVVRPAAAIEWTSSLALTRSTFRDPLRPTLDGRRVPQVPGRQ
                     FSSRLLASGTRVWASAELKHVGRQYEDDLNELPLSPASVIDVVAGWRVHRRVDVRVGV
                     ENVTGREVEVGRTPVSTIGLPRTLHVSVRIASRRAP"
BASE COUNT         1312 a         3303 c         3132 g         1408 t
ORIGIN      
        1 cgcttcttcc tgcttcttct tctcgtcctg ctttttcttt tcgtcctgct tcttcttctc
       61 gtcttgcttc ttcttgctgt cgccgttccc cttctcgtcg tccttcttcc cgtcgtcgcc
      121 gcccttgaag acgccgagca ggcggcccca gaagcccttc ttcttcttct tcggctcctc
      181 gctggccccc gtcgccttcg cctccgccgg gcggtcgtcg ccggcctcct cggcgcggcc
      241 gctcggccag gctgacggcg ccggggcgcc gccagccacc gtccgcgccc catccggcag
      301 gtcggtcgcc tcgccgaacg cgaaacgctg ctcgtgcagc gggcagtaac ccgagggcgc
      361 cgtgccccgg acgaagtact cggtgtagct gtacgaccgc tcctcggact gtccctcgtc
      421 gttcagcacc ggcacgtggt cgcatcccgg tgtcgccagc tgccccgaga tccggcagac
      481 gcggagggcc gtgagacccg agggcggcga gtaccacctg ggcttgtcat ccttggtcgc
      541 cgcgcccatg aaccgcgccc acagcggcac cgccacgtcg cccgcgtagc cgtcgcggac
      601 gatgggcctc ggcctgtcga accccaccca gacgccggtg agcaggctcg gtgtgtagcc
      661 gacgaaccac gcgtcctggt agtcgttggt cgtccccgtc ttgccggctg ccgggaggcg
      721 gaagcccatc tgccgtgccc ggtaggcggt gccgccgttg atcacgtcgg ccagcatcgt
      781 ggacatgaga aaggccgtct gctcgctgac cacccgggtg cccaggcgcg ccgcctcgta
      841 cagcaccttg ccgtcgcggt cttccacctt ggtgacgagc agcggcgggc gtgcgaggcc
      901 gccgtttgcg aacgggacgt acgacgaaga gagcgagagc agcgtcacct cgcccgaccc
      961 gagggccagc gatggcacgc tgggcacggt gccgagcccc atgcgctccg cgtaatccac
     1021 cgtccgcccg atgccgacgg tttgcagcat gcggacggcc gcgcggttgc tcgaggtccg
     1081 cagcgccgtg cggatcgtca tggtggacga ctcgaggtgc tcgtcctcgg gcacccactc
     1141 tccctcgggg gtgagcacgg ggtcgttgag gccgtcgagc agtgaggcgg gcgtgtagcc
     1201 cgactcgagc gcggccgcgt agaccaacgg cttgaacgcc gaccccggct gccgcctggc
     1261 ctgcatcgcg cggttgaagc ggctcagctc gaagtcgcgc ccgccgacca ttgcccgcac
     1321 ctccccggtg gcggggtcga ttgccaccag cgcggcctcg agcggctctt cttcgggtcc
     1381 ggccgcgtcg ggccgccggc ggctctcttt gcggcgccgc gcctcgagca gatcgagcgt
     1441 ctccttcacc gccgcctccg cgtgctgctg catcgacggg tcgatcgtcg tgtagacccg
     1501 gaggcctccc tcgtacacct gctcgaggcc gaagcggctc accagctcgc gccgcacctg
     1561 ctctttgaaa taccgccccc acggctcctc gcggcgcaag ccgtcgttga gggcgaccgt
     1621 ctcggcccgc gcccgcgcgt gctcgtcgcg gtcgatcgcg cccgtgtcca gcatcgcctg
     1681 cagaacgacg ttccggcgcg cgagcgcgcg ctgcaagttc acggtcggcg cgtaggccga
     1741 cggcgactgg acgaggcccg cgagcagcgc cgcctgcgac agcgtcagct ccgaggcgcg
     1801 ccggccgaag tacccgcggg cggccgcctc gatcccgtag aggccgtcgc cgaagtacac
     1861 cttgttcaag tacaactcga ggatctcgcg cttgctgaac agttgctcga tccggttcgc
     1921 gaggacgatc tcgcgcagct tcctccgcag cgtcttgtcc ggggtgagga accactggcg
     1981 cgccagctgc tgggtgatcg tgctcgcgcc ctgcgcggcc cggcgcgccc gcacgttggc
     2041 ccacatcgcg gcagccaggc gccacgggtc gtacccccgg tgcctgtaga accgctggtc
     2101 ctcgacggcg atgagcgcct ggagcacgag cggcgacaca tcgtcgagcc gcacctcgat
     2161 ccgctgttcc ttgctcaggc tgaaggcggg ctccccggcg cgatcgaaga tcgtcgtcgc
     2221 ctgcgccatc cttgtcatct gctgcagctc ggagcgcccg ggaaggccgg tgaggacgcc
     2281 ggctgcgaac cggaaggcct gcacgctcgc ggcgcccacg aggagcacga ccaggacgaa
     2341 agcagcggag atgagacccc gttgagggcc aaggttgtcg tacgtgcggc gaaggccgcg
     2401 gcggatgcgc cggatatcgg gacgggtgaa cgccataggt ctccacccac tcaatagcaa
     2461 ctcgcgcgcc acgggcgacc ccaccgcgcc gttgtggcac ctcagttgca aaaggaccgt
     2521 gcgggtcatc aagacggatc gggttcgggc ggcgccgccg agggcgggtg gcgtggcgag
     2581 cgaccccctc caccggcgct gccggaatgt gaatcggcat cgcgaggagg tcgggatcgg
     2641 cggccgaatc ggtccggggg agagccgcgg tgaggggtca gttgatgctt cgggcgttca
     2701 cgatctcggg cggggcgctg gcgatcatcg cgctcttcgt ccttggcgtc tccggcggtg
     2761 tgcagctcga gccccgggcc cggacggtcg tgccggcgcc gagcttgtcc gaaccgagaa
     2821 ttcctccgcc gcccggagcg tccgactcag ccgtccccat gttcccggcg ccgccgaggt
     2881 tgctggaggg caagacccgg acgacgctcg acggcgacga catcaacgac gccatcgcga
     2941 cctacggaat cgacgacgcc gggaacttgt acgaggtgca ctcgccgcac accgaggtgc
     3001 cgtcgctcgg cggtcctagg acctgacagg tcgtctgttt cattacccga cacgagcggg
     3061 taatgacgtt acggctgcct gaaaacggaa ctcgtccttt tgggcgcccg gccctcacgg
     3121 ggaccacagg gcggtagccc ggtcatacca ccgccgggac ctggttcagc gcgcgctccc
     3181 cggcttccca gcccctccgg aaagcctcca agttgaggtc gagcatgccg gggggaacag
     3241 aatccttcac agcttgttcg agtgcctcga aaggcatcag ctggctcacc gccccaaaga
     3301 acccgaccgt gatgatgttg aggacgatct tgcgcccgat ctcttccgcc agccgggtcg
     3361 cgggcaccac gaagtgcctc gcagcggaga gcggcggctc gggcttgacg aggtcggcct
     3421 cggtgaccag gaggcctccc gggcgcagcg cccggccgaa cttgttgtag gcgtcctggg
     3481 agagcacgac cagcatgtcg aggttcgtga cgtagggata ccagatcggc tcgttcgaca
     3541 cgatcagctc ggcgctcgac gagctgccgc gcgcctcggg cccgaagctc tggaccagcg
     3601 tggcgttgag gcccgcgtgg atggccgccg cgcggccgat gatgatgccg ctcaggatga
     3661 caccctgccc gccgaagccg ccgatgcgaa tctgggtctg cgccattact gactcgcccg
     3721 cgcgtaccgg tcgcccagct tgcgcgaata gaattggtcc atcgcgtcct ggtaggtcgg
     3781 acgctcgcgg tcgacgaact tccccaccac gatcggcccg gacatcccga gcgacacctc
     3841 gttggtggga gcgccgtgcc tgatgacgct ccgctgcttg taggtcttca cctcgtcgag
     3901 gccgtcgccg atccggttgc gccgcccgta gagcgtcggg cagggggaga tcacctcgac
     3961 gaacgagaac cccttcttga gcagcgcctc gcgcatcgcc ttcttcaggt gcgtcacgtg
     4021 gtaggtcgtc caccgcgcca cgtagaccgc gccgcacgac tcggccaggt acggcaggtt
     4081 gaacggctgc tcgaagttgc cgtacggcga cgtcgaggcg atggcctcct cgggcgtcgt
     4141 gggggtgagc tgaccgccgg tcatgccgta ggtcaggttg ttgacgcaga cgaccgtcat
     4201 gtcgaggttg cggcgcgcgg cgtggatgaa gtggttgccg ccgatggcga acaggtcgcc
     4261 gtcgcccgag tagacgacca ccttcatccc gggggcggcc agcttgaggc ccgtggcgaa
     4321 tgggatcgcg cggccgtgcg tcgtgtggaa cgagtccacg tgcatgtagc ccgcgacgcg
     4381 cccggtgcac ccgatgcccg acaccacggc caggcggtcc atcgggatgc cgctctcgtc
     4441 gatggcccgc gtgaagcagt tcacggtcgt cccgatgccg cagcccgggc accagatgtg
     4501 cgggatgcgc tcggagcgga ggtaccgttc gaccgggttc gcctgctcgc tcatcgcact
     4561 gcctccctga tcgcccgctc gatgacggcg gggtcgtgga ccgtgccgcc ggcgtgcgga
     4621 acggcgacca ctcgcgcggc gccggcgcag cgctcgacct cgcgcaccat ctgccccatg
     4681 ttcagctcgg gcaccacgaa cgcatgaggg cgggacgcca ggtcccggat aaggcgctcg
     4741 gggaacggcc acaccgtgcg caggcgcagc cagcccacct tcaacccgct cgcgcggcag
     4801 gcctcgatgg ccggcagggc gacgcgcgag gtgatcccgt agcacacgac cacggcgtcg
     4861 gcgccctcca cgccgtcctc gacgacatcc tgcagcgcgt ccgcgttctt cttgatcttg
     4921 tcggtgagcc gcgtgacgag ccgcgcctgc gccgcgaccg acatgtcggg atagccgcgc
     4981 tcgttgtgcg tcaggcccgt catgtggaag cggtagccct gcccggcgcg cgccatcgcc
     5041 ggcacgaggt cctcgccagg ggcgtacggg aggtactctc cgggcgccgc cttcgtccac
     5101 ttgcgcgggt agatctccag ctcctcgggc ctcggcacca caacccgctc catcatgtgg
     5161 cccacgcact cgtccatcat cacgagcacg ggaatgcggt actgctcggc gaggttgaag
     5221 gccttgaagg tgaggtcgaa gcactcctgc ggcgagttgg gcgacagcgc gacgatctcg
     5281 tagtcgccgt gcgagcccca ccgcgcctgc atcatgtcgg cctgcgccgg gagagtcggc
     5341 agcccggtcg aagggccgcc ccgctgcacg ttgatgaaga cgcagggcgt ctcggtcatc
     5401 accgcgaagc ccacgtgctc catcatcagc gagaagcccg ggcccgaggt gaccgtcatc
     5461 gcccgctttc cgccccagac ggcgcccgcg atggcgatcg acgaggccag ctcgtcctcc
     5521 atctggatga acacgcctcc cacctgcggg aagcgctggg cgatgcgctc gacgacctcg
     5581 gtggagggcg tgatcgggta gccggcgacg aatcggcact cggccgccag cgcgccttcg
     5641 caggccgcgt ggtcgccgtc gaggaagtgg gcgcccgtca ggacgccggc cggatcggca
     5701 tgcatcggaa acctctgggt gaaatgcgta cgtcggcccg ccggcaggcc gctcgacccg
     5761 cacggcctgc cagcgcctct ctcacgctag gggcggtagc cgtagatcgc gaagtccggg
     5821 cagtacatgc cgcacaggtc gcaccccgag caggcctcgc cccggacgac ctctggcggg
     5881 tggtagccct tggtggtgaa ggccggcgag agcgccagga cgtcggtcgg gcagaactcg
     5941 acgcagagcc cgcagccctt gcagcgctcg gcgtggatca cgacgacccc gcggacgcga
     6001 cgggtgggtt cagccatgcg gtgacctcgg gcgcaggccc gccccaggag cggaccccgg
     6061 gtgaaggcga tccatccgtc gatcgacctt ctcgcttctc tcagctgtcg tgcgctggaa
     6121 cccgcaggtg cgaggcctcg attggggaag ccaggcccac tgccgggggc gggacagtag
     6181 gggcgaccct gatggtcgtc ctactgcagg ctgccacaag gatagtgcca ggacggggat
     6241 ttatcaaagt gcccgcgccg cccggccggc cgccaggaac agggccagcg aggcggccgc
     6301 ggcgacgacc cagtcgcgcc gcatgtttaa ccagagctgc gggctggaac cggttgaccg
     6361 atcgtcgaag tcgccgtgcg cgccgtggtc gcccgggagc ggatcccaga ggttgtgagg
     6421 acggtccgga tcgatctcgc cgtcgtactg ctgggattcg tagccgttcc gcgcgaggta
     6481 gcggtcggcg taccaggccg cgagcttgtt gcccacgatc gcctggatcg tcggccaccc
     6541 gaagtagatc tcgcggcggc cggcgcgaga cgcgtaggag atcgcctcgg ccgccagctc
     6601 cggctggtag atcggcggca ccggctgcgg tctgcgcggc aggcgcgact tcacccacga
     6661 gaactgcggc gtgttcagcg ccggcagctg caccatcgtc aggtgcacgc ggctgccgtc
     6721 gtgcagcagc tcgctccaga gcgaatcgca gaagccctgg acggcgtgct tcgcggcgca
     6781 gtaggccgac tggagcggga tgccgcgata cgcgagcgcc gagcccacct gcacgatcgt
     6841 cccgcggtcg cgcggcagca tccggcggag cgcggcgagc gtaccgtgca cgacgccgag
     6901 gtaggtgacc tcggtcaccc gccggtactc ctccggggtc atctccttga caggggagaa
     6961 caccgaaacc atcgcgttgt tcacccagac gtcgatcggc cccagctcgc cctcgatccg
     7021 ttcggccgcc cgctccaccc ggctggcgtc ggccacgtcg gtgggcagaa cgagcgccgt
     7081 cccgcccgcc tcctcgacct cgcgccgcgc ggcctcgagg ccgtcgcgtc cgcgcgccag
     7141 cagcccgacg cgggccccgc gccgcccgaa cagccgcgcg gtggcccgtc ccacgccggc
     7201 cgaggcgccg gtgacgacga cgacctctgg ttgcatgccg gctacgggtt cagccggtcg
     7261 tggcccagct gtttcaccag cttgttgtac agcccgaaga tcagcaacgt cggcgcccac
     7321 tgccccacga agagcgccat ctcgcgcttc cccatgagct cgaagctgag agaggtcgcc
     7381 atcgatccga gagccgccca catgaaggtg tcggagggaa gcttcgccgt ctgctcttcg
     7441 atcgcacggg cgatcgggcc ttccgtctgc tgctcggccg ctctgattgg gttcgtcgtt
     7501 gccatgccgt ctccttggat gacgtccgcg tgtcctggtt tcccccggca gcccccggga
     7561 ctgcccggca gttcgggagg cagcaagcga ggtgccacga gttgtgacgc ttgagcgaga
     7621 cgagggccgc ttgcggttcg atccgctggg ccgcacggcc ggctaaggcg ctagaccggc
     7681 gcgtcgtggt tgtcaaggtg cgcgtcggct cgcgatgcgc acgctcacgt gaagggttcg
     7741 cggcaggccg atggtcgaga cgggcgtgcg ccccacttcc acctcgcgac cggtgacatt
     7801 ctccacgcca acccgcacat ctacgcggcg atgcacgcgc cacccggcga ccacgtcaat
     7861 caccgacgcg ggagagagcg gcagttcgtt caggtcgtcc tcgtactgcc ggcccacgtg
     7921 cttcagctcg gcactcgccc acacgcgtgt gccggaggcg agaagacgtg aggagaactg
     7981 ccggccgggt acctgcggca cgcgccggcc gtcgagcgtc gggcgcagcg ggtcgcggaa
     8041 cgtcgaacgt gtgagcgcga gcgacgaggt ccactcgatg gccgcggccg gccggacgac
     8101 gagctcggtt tcgagaccat acgcccgaat ctcgcccacg ttctggcgct gccgttcgcc
     8161 cggcccgatc gtcacgttcg cgaccgggtc gtcgaggcgg ctccaccagc ctgtcgcccg
     8221 caccgacagg cgccgcctgg tcatcatgat ccccgcctcg gtgccgctcg agcgctcggg
     8281 gcccaggctg tcgttcggtg ccgtgacaat cgatccgacc ctgaatccgc ggtacagctc
     8341 gttgagcgtc ggcgcacgga acgcccggta ggccgacgcg ttgaccacca cgcctggcga
     8401 cgcgtgccag gcgattgaga tccgcgggct caggctgctc gcccgtctcg actcgccgcc
     8461 agcggccgcc tggttcgccc acgtttccca tcgggctcct ccctcgatcg agaggcgccg
     8521 gccgagcggg gccgccatgc gcgcgaacac ggccgggccg cgctgccgtc cgcccgccgt
     8581 gcgggaaagg ccgccaaggg tcggagtctc cacgttgtcg gcgtgcacct gccgagcctc
     8641 gaacccggca accaccgcaa gcagcccgag gggctggttc cagctggcac gtccgccgac
     8701 cgcgtcgctg tccacatgct gcgtgatggt cggcgtctcc gagccgcgcc cgggcgcgac
     8761 cgtcgagaag gtctggccga agcgtgccct ctgaccccag gcgcgcacct cccacccgcc
     8821 ggcgccggca acgcccgacg ccgcgagcgc gagctgcacg ctgcgcgtgt cgttccgctg
     8881 cgcgggcgta ccgttgtcgc gatcctcgcc gaacgggttc acccgggcgg taccggtgaa
     8941 ggcgggcccg ctcgtccacg tgacggcgcc cagcgcggag gcgtggcgaa cgccggcctc
     9001 gacgtcgatc ggcccccggg cccgggccgc cacgattggt gcgcggccag cgcgcgccgc
     9061 ctcgatcgcc ccgatcgcgc tccacccgca tcgcgccacg cctgcgaacc ccgagacgcg
     9121 gcccgtcgcc cgcgacccgg cctcggctac gagcc
//
LOCUS       DAOPQX010000123         9144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8527_length_9144_cov_4.658708, whole genome shotgun sequence.
ACCESSION   DAOPQX010000123 DAOPQX010000000
VERSION     DAOPQX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8527_length_9144_cov_4.658708"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2695)
                     /locus_tag="PKK95_08285"
     CDS             complement(<1..2695)
                     /locus_tag="PKK95_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOC18250.1"
                     /translation="MLRPITGSTTKDTKGCARALIAAALALLAPAALLAQAPAAPVMQ
                     PATPLLGERIVEVRLIVEGRPVREPELRRALDTVEGAPLSASDVRESILHLMALRRFQ
                     DVQVSAEPAAGGVALTYAMQPREVVTGMDFEGDLGLSARRLRSTIIERWAGTPPLERA
                     DEAAAGLEAYLRERGYRGAKVSARTRSEGNYTAVLVFDVAAGPRVRVTSLRVDGPEDE
                     RERALAALGLSVGSPFDPGVALDRIDQYADRLRSRGFFDARAELVTAYSADGRGVDLV
                     VEVERGPHVTLVFRGDPIPREKREEVLPLDREGGLDEDRLEDASLEFERFLRGQGFRE
                     ATAPHSREQGPGGETLLVFTIRRGPQYKIADVVLEGVQQLPAATVRPMLATAPGQWFV
                     QSRVDADAARLAEEYRRRGFREVKVSAEVAPRAGSQSPLLDVRFTVAEGPRTTVGTFT
                     FEGNESVPATTLRGVVKSQPGAPYYQPQVNADRDAILSLYLDRGFQLASVEAQAQTAP
                     DSSRVDLLFQIQEGPRMVIDRVLVVGNQRVDALTIERETGLTRGMPVSPQVLADARRR
                     LASMGLFRRAQISELQLGSGTERDVLVEVEEGPVNTIGYGGGLEGLLRLKRDSGTGGT
                     VERFDVAPRGFFEFGRRNLWGGNRTINLFMRGAIRSSDLVNASRDDPSPPVTAPLDDE
                     GSGFREYRVLGTYREPGFMGLPIDLAVTGVVDQAIRSSYDFNRRQAFLEGSHRFSRFS
                     AAGRYSLGRTRVFNDRIAPEDRLNIDRLFNPGLRLSTFSVSAAFDTRDDPVSPTRGAL
                     VLFDTGLAARRFGSDMGFVRSYAQSFVFREIPKARGTVVAAGVRVGLARGFPRIIGQL
                     DDSGDPVLGPDGQPILVEVRDVPASERFFAGGDTTVRGFAQD"
     gene            complement(2695..6882)
                     /locus_tag="PKK95_08290"
     CDS             complement(2695..6882)
                     /locus_tag="PKK95_08290"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOC18251.1"
                     /translation="MTRLSSILRRWPEAALALVLAAVVVWAGFLPRSIALVLLGGVIV
                     FAVRRRVRDVVRAGVALAVILLLTAAATLVVDLGPALRRLAEVQGGRQIERPLHIARL
                     GIHLGSGRFVADGIRIEGLKPGDQPFFTAGRVSVGVPWWRVFRTREILIESIELADWV
                     MLIEDFEEGGSNFIHIPTPKRKRDDPNRIRTTLKYVHAGRGRLTYNDYGTWRTTAANL
                     DVVVERSGGRYLGRGSASNGSVLVEDYLPMRADIRFTFEIKGSIVEFDRIELHTDGTH
                     SMLTGRVDVGHWPEQTYQIRSRLDLWKMREVFFANDTWRSRGEATFDGTFHLFKGGHR
                     LAGDFACPLAFVNRLPFPRLRGSLEWEPDHFEVTRASALFQGGPMRFTYSMAPLGRPT
                     PALARFDVEYEGVDLGQLSDLFAFRGMRLEGRASGHNLLEWPLGGGWPQHRGGGVIRI
                     APPEGVVVMGREPADWEEEIHAEPPPIGPEPHPARTRRPLPVGGRFEYAFGPEWVDVA
                     PSHLATPRTYVEFEGRSAYSRRARYPFYARSADWLESDRVLASILTAFGSPTGVVAVG
                     GWGDFRGVMLGDFNDPRVEGVFTGEGMRAWDVVWGGGRAHLVIEDGYVDVTDGRVVRD
                     GGEIVANGRFSLGYPRADGGEEINARIAVAGRPLAEFRHAFQLDDWPVDGKVDGEYHL
                     YGRYQGPYGFGHMTVTEGTAWDEPFETATTPLRFEGTGVRLEGARVTKGTGVITAAAN
                     VIWEGRYSFNADGRNIPMESVAAVRNERAPLSGVLHFRANGAGEFLLPRYEVQVGIDD
                     LFVGDEGIGQAKAHLSFRDRLLTIAELEIASPRLAVSGTGRVEMNDTGDAELTFRFSD
                     TSLDPYVRAFEPRLSPFTRAVASGSIRVIGQLYDPGQLVVQADVESLDLRLFDYRLRN
                     DQALRISFDRNVARLEQVALVGEDTELRLSGEVDVGQDRIDVKAGGATNLAVLQGFFR
                     DIRSSGRAELSAAIRGPIRNPVFSGSATIRDGRVRYFGLPHSIQAINGEVTFGSNGIQ
                     MDGLTAQIANGSVRFGGSIEMAGYAPGRLGLSATAENMELRYPEGVRSVVDADLQLVG
                     TVSAPTVRGTINVKSAVMRRRFDLTPGLLELIAGRPAAPAGEERPAGTLPLRFDLRVF
                     APSALRIESNLMQILASGDLSLRGDYNHPVLLGRTEIERGQAVFEGKRYSLSGRIEFL
                     NPQRIEPTLEIEARTRVRAPGQTYDVDLRLLGTLQRLEPQFSSDPPLPPVEILSLLFG
                     GEPGRSQVADPELGSLQRAETQGRLGYSRLEQAVVGTLSSDVTRAVEQVFGLDSFQIT
                     PSFFDPYQRVQPTARLTVGKRISDRVYLTFSRSLNAPESDQVILLEYDQSDRLSWIFS
                     RNEDKTFALDVRVRHVF"
     gene            complement(6905..7432)
                     /locus_tag="PKK95_08295"
     CDS             complement(6905..7432)
                     /locus_tag="PKK95_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18252.1"
                     /translation="MTVAAMLFDIEIGERVHRVSVEREGARYRIVVDGRADVVDAARV
                     DSATLSLIVVGERHESHEVALLERGEPGALEVHLRAGVVAARVRPAGSRRGGAQAPGG
                     PGGPVKLTAPMPGKIVKVLVSPGDEVGPRQGLVVVEAMKMENELRAPRQGHVASVHVQ
                     EGALVEAGRLLLVIE"
     gene            complement(7473..8996)
                     /locus_tag="PKK95_08300"
     CDS             complement(7473..8996)
                     /locus_tag="PKK95_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOC18253.1"
                     /translation="MKVLIANRGEIAVRVIRACREMGLPSVAVYSDCDRASLHVRYAD
                     EAYPLGGNAPSESYLRIDALIEIARRSGADAVHPGYGFLAENEAFARACAAAGLVFVG
                     PPAEAIERMGSKTSARQAAIEAGVPVVPGTTGPLPPNATEAEISSVAGQVGYPIFVKA
                     VAGGGGKGMRLVTEPAELPSAVRAARSEARSSFGDPAIYIERQLQRPRHIEIQLLADA
                     SGTVVPFTERECSIQRRHQKVLEESPSVAVTPAKRRELADAAVAVARAVGYVNAGTIE
                     FLLDEDGRFYFLEMNTRLQVEHPITELVSGVDLVRWQLRIARGERLDLSPGLTLSPRG
                     HAVECRIYAEDPDNNFLPSAGLVLGRRVPGGPGIRRDDGGVTPGLEVPIYYDPLIAKL
                     TAWGGSRDEAVERLGRALGEYEVRGIKTSIPFFRWLFQNDDFRAGRFDTTFLDRELAA
                     RNGVPFVTIAAGAEHIALLAAAIHAFERAAGNAGAAPRGRAASAWTLRARSDGLRNG"
BASE COUNT         1310 a         3391 c         3221 g         1222 t
ORIGIN      
        1 ggtcctgggc gaagccgcgc accgtcgtgt ctccgccggc gaagaagcgc tcgctggcgg
       61 gcacgtcgcg cacctccacc aggatcggct gcccgtccgg cccgagcacc gggtcaccgc
      121 tgtcgtcgag ctgcccgatg atcctcggga acccgcgcgc caggccgaca cggacgccgg
      181 cggcgacgac cgtgccgcgc gccttcggga tctcgcggaa gacgaacgac tgcgcgtagc
      241 ttcgcacgaa gcccatgtcc gatccgaagc gccgggcagc caggccggtg tcgaacagca
      301 ccagtgctcc gcgcgtcgga gacaccgggt cgtcgcgggt gtcgaacgcg gcagagacgg
      361 agaaggtgga aaggcgaagc cccgggttga acagccggtc gatgttcagg cgatcctcgg
      421 gagcgatccg gtcgttgaac acacgcgtcc ggcccagcga gtaccgtccc gccgcgctga
      481 agcggctgaa gcggtggctg ccctccagga acgcctggcg gcggttgaaa tcgtagctcg
      541 acctgatggc ctggtccacc acgccggtga cggcgaggtc gatcggcagc cccatgaacc
      601 cgggctcccg gtacgtgccg agcacccggt actcccggaa ccccgatccc tcgtcgtcga
      661 ggggggcggt gaccggcggc gaggggtcgt cgcgcgaggc gttcaccagg tcgctcgagc
      721 ggatcgcgcc ccgcatgaac aggttgatcg tccggttgcc tccccagagg ttgcgccggc
      781 cgaactcgaa gaagccgcgc ggcgccacgt cgaagcgctc gacggtgccg ccggtgccgc
      841 tgtcgcgctt gaggcgcagc agcccctcga ggccgccgcc gtacccgatc gtgttgaccg
      901 gcccctcctc cacctccacc agcacgtcgc gctcggtgcc cgacccgagc tgcagctccg
      961 agatctgggc gcgccggaag agccccatcg aggccaggcg gcggcgcgcg tcggcgagga
     1021 cctgggggga aaccggcatg ccccgggtga ggccggtctc gcgctcgatg gtcagggcgt
     1081 ccacccgctg gttgcccacc acgagcacgc gatcgatcac catgcgcggc ccctcctgga
     1141 tctggaagag caggtccacg cggctgctgt cgggcgccgt ctgggcctgg gcctcgaccg
     1201 acgccagctg gaagccgcgg tcgagataca gcgagaggat cgcgtcgcgg tcggcgttga
     1261 cctgcggctg gtagtacggc gcacccggct gcgacttcac cacgccgcga agcgtggtgg
     1321 cgggcaccga ctcgttgccc tcgaaggtga aggtgccgac ggtcgtgcgc ggcccctcgg
     1381 ccaccgtgaa gcggacgtcg agcaggggcg actgcgaccc ggcgcgcggc gccacctcgg
     1441 ccgagacctt cacctcgcgg aagccgcggc gtcggtactc ctcggcgagg cgggcggcgt
     1501 cggcgtccac gcgggactgc acgaaccact gccccggggc cgtcgcgagc atcgggcgca
     1561 ccgtcgccgc cggcagctgc tgcacgccct cgaggacgac gtccgcaatc ttgtactgcg
     1621 ggccgcggcg gatcgtgaac acgagcagcg tctcccctcc cgggccctgc tcgcgcgagt
     1681 gcggcgcggt cgcctcacgg aagccctggc cgcgcaggaa gcgctcgaac tcgaggctgg
     1741 cgtcctcgag gcggtcctcg tcgagtcccc cctcgcgatc gagcggcagc acctcttcgc
     1801 gcttctcgcg cgggatcggg tccccgcgga acaccagggt gacgtggggg ccgcgctcga
     1861 cctccacgac gagatcgacg ccgcgcccgt cggcggagta ggccgtcacc agctcggccc
     1921 tcgcgtcgaa gaaaccgcgg ctcctgagcc ggtcggcgta ctgatcgatc cgatcgaggg
     1981 cgacgccggg gtcgaaggga gagccgaccg acaggccgag ggccgcgagc gcgcgctcgc
     2041 gttcgtcctc gggcccgtcc acccggagcg acgtcacgcg cacgcgggga ccggcggcga
     2101 cgtcgaagac gaggacggcg gtgtagttcc cctccgaacg ggtacgggcc gacaccttcg
     2161 cgccgcggta tccccgctcg cgcaggtagg cctcgaggcc cgcggccgct tcatcggcac
     2221 gctcgagcgg cggcgtcccg gcccaccgct cgatgatcgt gctgcgcagg cggcgtgcgc
     2281 tcaggccgag gtcaccctcg aagtccatcc cggtgacgac ctcgcgcggc tgcatcgcgt
     2341 aggtcagggc aaccccgccg gcggcgggct cggccgagac ctgcacgtcc tggaagcgcc
     2401 gcagcgccat caggtgcagg atgctctcgc gcacgtccga ggccgagagc ggcgccccct
     2461 cgaccgtgtc gagcgcgcgc cgcagctcgg gctccctgac gggacgcccc tcgacgatga
     2521 gacggacctc gacgatgcgc tcgccgagga gtggcgttgc cggctgcatc acgggcgccg
     2581 cgggggcctg cgccagcagc gcggccggcg cgagcagggc taaggccgca gcaatgaggg
     2641 cgcgcgcgca cccctttgtg tcctttgtgg tcgaacccgt gatcggtcgc aacatcagaa
     2701 gacgtgcctc acgcggacat cgagggcaaa cgtcttgtcc tcgttgcgcg agaagatcca
     2761 cgagagccgg tcgctctggt cgtactcgag caggatgacc tggtcgctct cgggggcgtt
     2821 caggctgcgc gagaacgtca ggtacacccg gtcggagatc cgcttgccga ccgtcagcct
     2881 cgcggtgggc tgcacgcgct ggtaggggtc gaagaaggac ggcgtgatct ggaacgagtc
     2941 gaggccgaac acctgctcga ccgcgcgcgt cacgtcgctc gagagcgtgc cgacgaccgc
     3001 ctgctccagg cgcgagtagc cgagccgccc ctgcgtctcg gcccgctgca gcgagcccag
     3061 ctccgggtcg gcgacctggc tgcgccccgg ctcgcccccg aagagcagcg acaggatctc
     3121 gacgggcggc agcggcggat cggaggagaa ctgcggctcg agcctctgca gcgtcccgag
     3181 caggcgcagg tccacgtcgt aggtctgccc cggggcgcgc acgcgcgtcc gcgcctcgat
     3241 ctcgagcgtc ggctcgatcc gctgcgggtt caggaactcg atgcggccgg agaggctgta
     3301 ccgcttcccc tcgaacaccg cctgaccccg ctcgatctcg gtccggccga gcagcaccgg
     3361 gtggttgtag tcgccgcgca gcgacaggtc gccgctcgcg agaatctgca tcaggttgct
     3421 ctcgatgcgc agcgccgacg gcgcgaacac ccggaggtcg aaccgcagcg gcagcgtccc
     3481 agcgggccgc tcctcgccgg cgggggccgc cggccggccg gcgatcagct cgagcaggcc
     3541 cggcgtcagg tcgaaccgcc tccgcatcac cgcgctcttc acgttgatgg tcccgcgcac
     3601 ggtcggggcc gagacggtgc caaccagctg caggtcggcg tccaccaccg agcgcacgcc
     3661 ctcggggtag cgcagctcca tgttctcggc ggtcgcgctg agccccagcc gcccgggcgc
     3721 gtagccggcc atctcgatgc tgcccccgaa gcgcaccgac ccgttcgcga tctgcgccgt
     3781 caggccgtcc atctggatgc cgttgctccc gaaggtgacc tccccgttga tcgcctggat
     3841 cgagtgcggc aggccgaagt agcggacgcg gccgtcgcgg atcgtcgcgc tgcccgagaa
     3901 gacggggttg cggatgggtc ctcggatcgc ggcgctcagc tcggcgcgcc ccgagctgcg
     3961 gatgtcgcgg aaaaacccct gcaggaccgc gaggttggtg gcgccgcccg ccttcacgtc
     4021 gatccggtcc tgcccgacgt ccacctcgcc cgacagccgc agctcggtgt cctcccccac
     4081 cagggccacc tgctccaggc gcgccacgtt ccgatcgaag gagatccgca gcgcctggtc
     4141 gttccgcagc cggtagtcga acagccgcag gtcgagggac tcgacgtcgg cctgcaccac
     4201 cagctgcccg ggatcgtaca gctgcccgat cacccggatc gagccgctcg ccacggcgcg
     4261 ggtgaacggg gacagccgcg gctcgaacgc gcggacgtac gggtcgagcg acgtgtcgct
     4321 gaaccggaac gtcagctcgg cgtcgccggt gtcgttcatc tcgacgcggc ccgttcccga
     4381 cacggcaagg cgcggcgagg cgatctccag ctcggcgatc gtcagcagcc ggtcgcggaa
     4441 cgacaggtgc gccttcgcct ggccgattcc ctcgtcgccg acgaacaggt cgtcgatccc
     4501 gacctgcacc tcgtagcgcg gcagcaggaa ctcccccgcc ccgttcgccc ggaagtgcag
     4561 cactcccgag agcggcgccc gctcgttccg cacggcggcc accgactcca tcgggatgtt
     4621 gcggccgtct gcgttgaacg agtaccgccc ctcccagatg acgttcgcgg cggcggtgat
     4681 gacgccggtg cccttggtca cccgcgctcc ctcgaggcgc acgccggtcc cctcgaagcg
     4741 cagcggcgtg gtggccgtct cgaacggctc gtcccacgcg gtcccctcgg tcaccgtcat
     4801 gtggccgaac ccgtagggcc cctggtaccg gccgtagagg tggtactcac cgtccacctt
     4861 gccgtccacg ggccagtcgt ccagctggaa ggcgtggcgg aactcggcga gcgggcggcc
     4921 ggcgaccgcg atccgcgcgt tgatctcctc cccgccgtcc gctcgcgggt agccgagcga
     4981 gaaccggccg ttcgccacga tctcgccgcc gtcacggacg acgcggccgt cggtcacgtc
     5041 cacgtagccg tcctcgatca ccaggtgggc gcgcccgccg ccccagacga cgtcccacgc
     5101 gcgcatcccc tcgccggtga agaccccctc gacccgcggg tcgttgaagt cgccgagcat
     5161 caccccgcgg aagtcgcccc agccgcccac ggccacgacg ccggtcggcg atccgaacgc
     5221 ggtgaggatg gaggcgagca cgcggtcgct ctccagccag tcggcgctgc gcgcgtagaa
     5281 gggatagcgt gcgcgcctgg agtaggccga ccggccctcg aactcgacgt aggtgcgcgg
     5341 cgtggcgagg tggctcggcg cgacgtccac ccactcgggc ccgaaggcgt actcgaaccg
     5401 gccgccgacc ggcagcggcc tccgcgtgcg ggcggggtgc ggctcgggcc cgatcggcgg
     5461 cggctcggcg tgaatctcct cctcccaatc ggccggctcg cgccccatca ccacgacgcc
     5521 ttcgggcggc gcgatccgga tgacgccgcc gccgcggtgc tgcggccacc cgcccccgag
     5581 cggccactcg agcaggttgt gcccgctggc ccgcccctcg agccgcatgc cccggaaggc
     5641 gaagaggtcg gacagctggc cgagatccac cccctcgtac tcgacgtcga aacgcgcgag
     5701 cgccggcgtc ggccggccga gcggcgccat cgagtaggtg aagcgcatcg ggccgccctg
     5761 gaagagcgcc gacgcccggg tcacctcgaa gtgatccggc tcccactcga gcgacccgcg
     5821 caggcggggg aacggcagcc ggttgacgaa cgcgagggga caggcgaagt cgcccgcgag
     5881 gcggtgcccc cccttgaaca ggtggaaggt cccgtcgaag gtcgcctcgc cgcggctgcg
     5941 ccaggtgtcg ttggcgaaga acacctcgcg catcttccag aggtccagcc gcgagcggat
     6001 ctggtaggtc tgctcgggcc agtgccccac gtccacccgg ccggtgagca tcgagtgcgt
     6061 gccgtcggtg tgcagctcga tccggtcgaa ctcgacgatg ctgcccttga tctcgaaggt
     6121 gaagcggatg tccgcccgca tcggcaggta gtcttccacc agcacgctgc cgttggaggc
     6181 cgacccgcgg cccaggtacc gcccgcccga gcgctccacg acgacgtcga ggttcgccgc
     6241 ggtggtgcgc cacgtgccgt agtcgttgta ggtgagccgg ccgcgccccg cgtgcacgta
     6301 cttcagggtc gtcctgatgc ggttgggatc gtcgcgcttc ctcttcggcg tcgggatgtg
     6361 gatgaagttg ctcccgccct cctcgaagtc ctcgatcagc atgacccagt cggccagctc
     6421 gatcgactcg atgaggatct cccgcgtgcg gaagacgcgc caccagggaa cgccgaccga
     6481 cacgcggccc gcggtgaaga acggctggtc gcctggcttc agcccctcga tgcggatgcc
     6541 gtcggccacg aagcggccgc tgccgaggtg gatgccgagc cgggcgatgt gcagcggacg
     6601 ctcgatctgg cggccgccct ggacctcggc cagccggcgc agcgccggcc ccaggtcgac
     6661 gacgagggtg gcggccgccg tcaacaagag gatgacggcc agggcgacgc ccgcccgcac
     6721 gacgtcgcgc acgcgccggc ggaccgcgaa cacgatcacg ccgcccagca ggacgagggc
     6781 aatcgacctg ggcaggaagc ccgcccacac gacgacggcc gcgagcacca gggccagtgc
     6841 cgcctcgggc cagcgacgga ggatggacga cagtcgggtc acctcgaaag cagcctcgaa
     6901 cgcttcactc gatcacgagg agcaggcggc ccgcctcgac cagcgcgccc tcctgcacgt
     6961 gcaccgacgc cacgtggccc tgccgcgggg cgcgcagctc gttctccatc ttcatcgcct
     7021 cgacgacgac taggccctgg cgcgggccca cctcgtcgcc cggcgacacg agcaccttga
     7081 cgatcttccc gggcatcggc gccgtgagct tgaccggtcc gcccgggccg cctggcgcct
     7141 gcgccccgcc gcgccggctg ccggccggcc tcacgcgcgc ggcgacgacc ccggcgcgca
     7201 ggtgcacctc cagcgcgccc ggctcgccgc gctcgagcag ggccacctcg tggctctcgt
     7261 gccgctcgcc cacgacgatc agcgacaggg ttgcgctgtc cacgcgggca gcgtccacga
     7321 cgtcggcgcg gccgtccacc acgatgcggt agcgggcgcc ttcccgctcg accgacacgc
     7381 ggtgcacgcg ctcaccgatc tcgatgtcga acagcatcgc ggcaaccgtc acgctgtcac
     7441 gtgtccacgt tcaccacacc ctgatccctg ccctacccgt ttctcagccc gtcgctccgc
     7501 gccctcagcg tccacgcgct cgcggcccgt ccgcgcggcg ccgcgccggc gttcccggcg
     7561 gcccgctcga aggcgtggat cgccgcggcc aggagcgcga tgtgctcggc cccggccgcg
     7621 atggtcacga acgggacgcc gtttcgcgcg gccagctcgc ggtcgaggaa cgtcgtgtcg
     7681 aagcgcccgg cccggaagtc gtcgttctgg aacagccacc ggaagaacgg gatgctcgtc
     7741 ttgatcccgc gcacctcgta ctcgccgagc gcgcgcccga gccgctccac cgcctcgtcg
     7801 cggctgcccc cccacgcggt cagcttggcg atgagcgggt cgtagtagat cggcacctcg
     7861 aggccgggcg tcacgccccc gtcgtcgcgc cggatgccgg ggccgccggg aacgcggcgc
     7921 ccgaggacga ggccggccga cgggaggaag ttgttgtcgg gatcctccgc gtagatccgg
     7981 cactcgaccg cgtggccgcg gggcgagagc gtcagcccgg gcgagagatc gaggcgctcg
     8041 ccgcgtgcga tccgaagctg ccagcgcacc aggtcgacgc ccgacaccag ctcggtgatc
     8101 gggtgctcga cctggaggcg ggtgttcatc tcgaggaagt agaaccggcc gtcctcgtcc
     8161 agcaggaact cgatcgtccc ggcgttcacg tagcctactg cccgcgcgac cgccaccgcg
     8221 gcgtcggcca gctcgcgccg cttggcgggc gtgaccgcca ccgagggcga ctcctcgagc
     8281 accttctgat ggcggcgctg gatggagcac tcgcgctcgg tgaacggcac gacggtgccc
     8341 gacgcgtcgg cgagcagctg aatctcgatg tgccgtggcc gctgcagctg gcgctcgatg
     8401 tagatggccg ggtcgccgaa cgaggagcgc gcctcggagc gggcggcgcg cacggccgac
     8461 ggcagctcgg ctggctcggt caccaggcgc atccccttcc cgccgccgcc ggcgaccgcc
     8521 ttcacgaaga tcgggtagcc cacctgcccg gccaccgacg agatctcggc ctcggtcgcg
     8581 ttaggaggca ggggcccggt cgtgccgggc acgaccggca cgcccgcctc gatggccgcc
     8641 tgccgcgccg aggtcttgct gcccatgcgc tcgatcgcct cggccggcgg cccgacgaag
     8701 accagccccg ccgcggcaca ggcgcgcgca aatgcctcgt tctcggccag gaagccgtag
     8761 cccgggtgca cggcgtcggc cccggaccgg cgcgcgatct cgatgagcgc gtcgattcgc
     8821 aggtagctct cggaaggggc gttgccgccc agcgggtacg cctcgtccgc gtagcggacg
     8881 tggaggctgg cgcggtcgca gtccgagtag acggcgacac tcggcaggcc catctcgcga
     8941 cacgcgcgga tgacccgcac ggcgatctcg ccacggttgg cgatcagcac cttcatgagg
     9001 atggcgatgc tgccgataac gtcctagcat tctagcattt ggaacgtgga ggatggctgc
     9061 ggcgggcccg gatctgggcg ccatctgcgc ccgagtggca caggcgtccc gcctgtggat
     9121 ggtggtcgca gtggccacag gcgg
//
LOCUS       DAOPQX010000124         9093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8589_length_9093_cov_4.475326, whole genome shotgun sequence.
ACCESSION   DAOPQX010000124 DAOPQX010000000
VERSION     DAOPQX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9093
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8589_length_9093_cov_4.475326"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1812)
                     /locus_tag="PKK95_08305"
     CDS             complement(43..1812)
                     /locus_tag="PKK95_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18254.1"
                     /translation="MRPRPARGRRALLWAPAAAVAVLLGIGLLLPPKALVLRQPEWRP
                     AGTTWRGAIHVHTNRSDGTGTIDEVAKAASRAGLDFVVLTDHGDGTRPPEPPAYRSGV
                     LVLDGVEIGTSAGHYLAIGMTRAPYPLGGDARDVVEDVARLAGFGVIAHPDSAKDGQG
                     WQHWDLPADGIEWLNTDSQWRDEGGLRLARAVLTYPFRPAETLASQLNQGQLLARWDR
                     AASSRPLVALAGADAHARIGLSGGGDPYEGRPFLAVPSYEASFRAFSLQLVTPGPRSG
                     RAEEDARAVLASLRAGRLHTVIDGWATPAIFEFSARGGGIEVAEGGEVRAADPMVMHV
                     RSNAPLYAAVVLLRDGVEVHRVDRQSMVYATDRPGRYRVEIWLGRPGKRSPIPWLVSN
                     AITLRKTSAVAGSVPGGAEVDGRAVIPEEGQAGGETWAIAPRWHVEHDPRSTGELIPA
                     TERPGESSMRFRLGPGTPAGQFAALVTPVRLDAGARAIVFSGQALEPMRVSVQLRAPS
                     GLEGERWRRSVYLDNNPREVRVALDEMTPAGPTRTHTVSLPLADNLLFVVDTVNTAPG
                     TAGEFTVSGLRVEGESTDRPASQ"
     gene            complement(1809..2960)
                     /gene="metK"
                     /locus_tag="PKK95_08310"
     CDS             complement(1809..2960)
                     /gene="metK"
                     /locus_tag="PKK95_08310"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOC18255.1"
                     /translation="MSRDGRHLFTSESVTEGHPDKIADQISDAILDAILAQDPVGRVA
                     CETLVTTGLAMIAGEITTSCYVDFPKIVRETIREIGYTRAKYGFDSDTCAVLSSIHEQ
                     SPDIAMGVDPGGAGDQGLMFGFACTETDELMPMPIMLAHKLCMRLSAVRRSGELDYLR
                     PDGKSQVTIEYDNGRPVRVDAVVVSAQHSALVDRETMQEEVLERVIDPVVPANMRDER
                     TRIFINPTGRFVIGGPQGDAGVTGRKIIVDTYGGAAPHGGGAFSGKDPTKVDRSACYM
                     ARYIAKNCVAAGLADRLLVQLAYAIGVADPVSVMVDTYGTGRVDDQCITELVRSHFKL
                     TPRGIMEELDLRRPIFRKTAAFGHFGRNDPDFTWERTDRAAALRADAGL"
     gene            3061..3966
                     /gene="xerD"
                     /locus_tag="PKK95_08315"
     CDS             3061..3966
                     /gene="xerD"
                     /locus_tag="PKK95_08315"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOC18256.1"
                     /translation="MTKGDDRSAALADAYLDHLRVERRLSPLTIESYARDLAALGRFL
                     ESEGLSELACTRQDLEAFVRSLMASGLSPRSTARAVACVRGFFRFLVVDRRAAANPAD
                     ELRAPRAWPALPRFLTLDQVDRLIAQPDVDTPRGLRDRTMIEVLYATGVRVSELIGLR
                     PPDLHLDGGYLTCVGKGGKERLVPLGDEAASWVARYLRESRPRLLKGRTSPRLFVNGR
                     GGPLSRVGFWKILKGHALAAGLPSEVSPHVLRHSFATHLLDRGADLRAIQLMLGHADL
                     STTQIYTHVLETRMRAVYERFHPRR"
     gene            4054..5040
                     /gene="murQ"
                     /locus_tag="PKK95_08320"
     CDS             4054..5040
                     /gene="murQ"
                     /locus_tag="PKK95_08320"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOC18257.1"
                     /translation="MALQSKWQQLPTEAINPATLGIDKLSPAGIIDLMLDEDRRIISA
                     VAREKERIALGVEMLAQAVRRGGRIVFVGAGTSGRLGVLEAAEMPPSFSTSPSLVQAV
                     IAGGKKAITRDVEGAEDDYEAGARALARLRPGAKDVVIGVSASGMTPFVRGALSRARK
                     AGARIVFVTCFPGTELQTFVDLTIAPAVGPEVIAGSTRLKAGTATKIVLNMLTTGAMV
                     RTGKTYGNLMVDLKTGSEKLKDRARRILTFVSGIDYDTADRLLKRAHWNVKAAIVMQK
                     TGLPFRQALSKLRQADSSVRAAIDEDAEPRLRQLLGSRAGDPDKEKEREKEK"
     gene            5065..6303
                     /locus_tag="PKK95_08325"
     CDS             5065..6303
                     /locus_tag="PKK95_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOC18258.1"
                     /translation="MPLRPSVAVLVACLAGLTAAAPLAQAPAPRATRAEIRALWVART
                     SLTSPRAVEEMVSSARAAGFNTLLVQVRGRGDAYFADGMEPRAATLASQPESFDPLAR
                     TLALARPLGLHVHAWVNVNLVSSAADLPSSREHVVYRHPEWLMVPREIARDMSLLNPK
                     SLLYLDKLARWARSQPGEIEGLYLSPIPAGAADRTVQVIADLVTRYPVDGLHLDYVRY
                     PSGAFDYSREALEAFRDSLLEGLDARERQRRGQAIGADIVRWAERHPAEWEAFRRARL
                     TALVSRIRESLARRRPGALLSAAVYPDAARAASERLQDWSGWIDRRLLDVVCPMAYAT
                     DADAFTEQLTAAKRAARDLPVWAGIGAYRLSTEQTIENIRIARRLGASGVVLFSYDSL
                     VASPKGPGYLSQVSRAVFGG"
     gene            6334..7389
                     /locus_tag="PKK95_08330"
     CDS             6334..7389
                     /locus_tag="PKK95_08330"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC18259.1"
                     /translation="MPRVAGAAGFSRAKGVLDEAVSRRVFPGAAAEAGGPGGPAWQYA
                     TGTLSYDASSPPATIDTVYDLASLTKVLCTTTLVMRQAHSGRLPLETPLSALLPAWRG
                     GGRDVVTLADLLAHASGLPAWLPLYETCAGRAQFERAIAAVPLAGPPRSVSVYSDLGF
                     MLLGFVLEDVGGTSLARQFEALEADGLGFGVAPGDRARTAPTQQDEWRGRLLRGEVDD
                     RNAHALGGTAGHAGLFGTIGGVGMLARRLMQGLEGEEAALGVTPDTLRAFVTRVAIPG
                     SSRALGWDTMLPTSSCGSRMSPRAFGHTGFTGTSLWIDPEKRFYAVLLSNRIHPKPGD
                     PAEILAVRQAFHDALAG"
     gene            complement(7409..8200)
                     /locus_tag="PKK95_08335"
     CDS             complement(7409..8200)
                     /locus_tag="PKK95_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18260.1"
                     /translation="MQGQAPRSESGYAMAAMLVALGVMSIMLMVAMPAWRQMVQREKE
                     EELIFRGQQYARSIGLFQRKYAAAYPPDIDTLVQQKFLRKKYKDPMVPDGEFQVLYQA
                     SMGQPMVPGRQPGFGQQGRGEGLSGVRREQGSQLGVARDQARRDDQAQAPRTSAFANR
                     PAGGLSGTVGPRGGVIGVTSKSTEKSIRLYNGRDRYSEWQFVYTPSLFGPGQPRPGMR
                     PGQPRPGARPGQMPGRPGFGTPGTPGAPGNRPGGVRPPTGGNPQQ"
     gene            8232..>9093
                     /locus_tag="PKK95_08340"
     CDS             8232..>9093
                     /locus_tag="PKK95_08340"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC18261.1"
                     /translation="MIPDERPHRVPRSAGRRVCRAAVLIVLSSVLAIQACDKVALVAP
                     TGTTITLYTNSQFLAVNGTADITASVIEANGYPVQNGTTVTFTTSLGTVEPSDAKTHD
                     GKATVKLLIGTAAVGQVLVSATPPTVPATGGTVQIVATVQDTGGNNLLGVPVNFTTDF
                     GTLVPDTAVSDSNGNARTVLTTSQSATVTATAGAKNATAKVTALAVPTITITPPSTTP
                     SVGVPASFTINVTAGANSSPITDVTVDFGEGRPVSLGAVNGSITVSHTYDVADDYTVT
                     VTARDATGVTA"
BASE COUNT         1270 a         3334 c         3203 g         1286 t
ORIGIN      
        1 ctaatcgcga atgggtcatt cgaccaattg tgtagccgca catcactgcg aagctggcct
       61 gtcggtgctc tccccttcca cgcgcaaccc gctgacggtg aactcccctg ccgtccccgg
      121 ggcggtgttc acggtgtcga ccacgaagag gaggttgtcg gcgagaggca gcgacaccgt
      181 gtgcgtccgg gtgggtccgg ccggtgtcat ctcgtcgagg gcgactctga cctcgcgcgg
      241 gttgttgtcg aggtagaccg agcggcgcca ccgctccccc tccagacccg acggcgcccg
      301 cagctgcacc gagacccgca tcggctcgag ggcctgcccg ctgaagacga tcgcccgcgc
      361 accggcgtcg agccggaccg gcgtcaccag cgccgcgaac tgccccgcag gcgtgcccgg
      421 cccgaggcgg aagcgcatcg acgattcccc ggggcgctcg gtcgcgggaa tcagctcgcc
      481 ggtcgagcgc gggtcgtgct cgacgtgcca ccgcggcgcg atcgcccatg tctctccgcc
      541 cgcctggccc tcctcgggga tgacagcccg cccgtccacc tcggcacccc ctggaacgga
      601 gccagcgacg gcagacgtct tcctgagcgt gatcgcgttg ctcacgagcc acgggatcgg
      661 ggagcgcttg cccggccggc ccagccagat ctccacccgg tagcggcccg gcctgtcggt
      721 cgcgtacacc atggactggc gatcgacgcg atgcacctcg acgccgtcgc gcagcagcac
      781 gacggcggcg tacagcggcg cgttgctgcg aacgtgcatc accatcgggt cggccgccct
      841 cacctccccg ccctcggcca cctcgatccc gccgccgcgc gcgctgaact cgaagatcgc
      901 cggggtggcc cacccgtcga tcaccgtgtg cagccgtccg gctcgcagcg aggccaacac
      961 ggcccgcgca tcctcttcgg cacgcccgct gcggggcccc ggcgtgacca actgcagcga
     1021 gaaagccctg aagctggcct cgtacgacgg cacggcgaga aacggccggc cctcgtacgg
     1081 atcgcccccg cccgagaggc cgatgcgggc gtgcgcgtcg gcacccgcga gggcgaccag
     1141 cggccgcgac gaggcggcac ggtcccaccg cgcgagcagt tgtccctggt tcagctgcga
     1201 ggcgagcgtc tcggccggcc tgaacgggta ggtcagcacg gcgcgggcga gccgaagccc
     1261 gccctcgtcg cgccactggc tgtcggtgtt cagccactcg atcccgtcgg ccgggaggtc
     1321 ccagtgctgc cagccctggc cgtccttggc cgagtcgggg tgcgcgatca cgccgaaccc
     1381 cgcgaggcgc gcgacgtcct cgaccacgtc gcgggcgtcg ccgccgagag gatacggcgc
     1441 gcgggtcatg ccgatggcca ggtagtgccc cgcgctggtg ccgatctcga cgccgtcgag
     1501 cacgaggacg cccgaccggt aggcgggcgg ctcgggcggc cgggtgccgt cgccgtgatc
     1561 ggtgaggacg acgaagtcga ggccggcgcg cgaggcggcc ttggccacct cgtcgatcgt
     1621 gccggtgccg tcggaacggt tggtgtggac gtggatggcg ccgcgccacg tggtgccggc
     1681 ggggcgccat tcgggctgcc ggaggacgag cgccttcggc gggagcagca acccgatgcc
     1741 gagcagcacg gcgaccgccg cggccggggc ccagaggagg gcccgcctgc cgcgcgcggg
     1801 gcgcggcctc acagacccgc gtcggcccgc agcgcggccg cgcggtcggt gcgctcccag
     1861 gtgaagtcgg gatcgttccg gccgaagtgc ccgaaggcgg cggtcttgcg gaagatcggc
     1921 cggcgcaggt cgagctcttc catgatcccg cgcggcgtga gcttgaagtg cgaccgcacc
     1981 agctcggtga tgcactggtc gtcgacgcgg ccggtgccgt aggtgtcgac catgaccgac
     2041 accgggtcgg cgacgccgat cgcgtaggcg agctgcacga gcaggcggtc ggccagcccc
     2101 gcggcgacgc agttcttggc gatgtaccgc gccatgtagc aggcggagcg atcgaccttt
     2161 gtgggatcct tgcccgagaa ggcgccgccg ccgtgcggcg cggccccgcc gtaggtgtcg
     2221 acgatgatct tgcggcccgt gacgccggcg tcgccctgtg ggccgccgat gacgaagcgg
     2281 ccggtcgggt tgatgaagat ccgggtgcgc tcgtcccgca tgttggcggg caccaccggg
     2341 tcgatgaccc gctcgagcac ctcctcctgc atcgtctcgc gatcgaccag ggcgctgtgc
     2401 tgagccgaca cgaccacggc gtccacgcgc acggggcggc cgttgtcgta ctcgatggtc
     2461 acctgcgact tcccgtcggg gcgcaggtag tcgagctcgc ccgagcggcg gacggccgac
     2521 agccgcatgc acagcttgtg ggcgagcatg atcggcatcg gcatcagctc gtcggtctcg
     2581 gtgcaggcga agccgaacat gagcccctgg tctccggcgc cgcccgggtc gacgcccatc
     2641 gcgatgtctg gcgactgctc gtggatcgac gagaggacgg cgcaggtgtc ggagtcgaag
     2701 ccgtacttcg cgcgggtgta gccgatctcg cggatcgtct cccggacgat cttcgggaag
     2761 tcgacgtagc agctggtggt gatctcgccg gcgatcatcg cgaggccggt cgtcaccagc
     2821 gtttcgcagg cgacgcgccc gaccgggtcc tgcgcgagaa tcgcgtcgag gatcgcgtcg
     2881 gagatctggt cggcgatctt gtcggggtgc ccctcggtca ccgactcgga cgtgaacagg
     2941 tggcggccgt cacggctcat cgcgatcctc ccggaaatgc cgaacgctcg acgggtccac
     3001 ctaggctact gcggggtgcc gcttgacgca agtgcgcacg cggccccaag ctgagggcgc
     3061 atgacgaagg gcgacgaccg ctccgcggcg ctcgccgacg cctacctgga tcacctccgg
     3121 gtggagcgcc ggctctcccc cctcaccatc gagagctacg cgcgcgacct cgccgcgctc
     3181 ggccgcttcc tcgagagcga ggggctctcg gagcttgcct gcacgcgcca agacctggag
     3241 gcgttcgtcc gcagcctgat ggcctcgggc ctctcgcccc ggtccaccgc gcgcgcggtc
     3301 gcctgcgtcc ggggcttctt ccgctttctc gtcgtcgacc ggcgggcggc ggcaaacccg
     3361 gccgacgagc tgcgcgctcc gcgcgcgtgg cccgcactcc cccggttcct gacgctcgac
     3421 caggtggacc ggttgatcgc gcagcccgac gtggacacgc cgcgcggcct gcgcgaccgc
     3481 acgatgatcg aggtgctcta cgcgacgggc gtgcgcgtct cggagctgat cggactgcgg
     3541 ccgcccgacc tccacctgga cgggggttac ctgacctgcg tgggcaaggg cggcaaggag
     3601 cggctggtgc cgctcggcga cgaggcggcc agctgggtcg cgcgttacct gcgcgagtcg
     3661 cgcccgcgcc tgctcaaggg acgaacctcg ccgcggctct tcgtgaacgg gcgcggcggc
     3721 cccctctcgc gcgtgggctt ctggaagatc ctcaagggcc atgccctcgc ggccggcctg
     3781 ccctccgagg tcagcccgca cgtgctccgc cactcgttcg ccacgcacct gctcgaccgc
     3841 ggcgccgacc tccgcgcgat ccagctcatg ctgggccacg ccgacctctc gaccacgcag
     3901 atctacacgc acgtgctcga gacccggatg cgcgccgtgt acgaacgctt ccacccgcgg
     3961 cgctgacgcg cgccgacgag ggctgcgctt cgcggggcgc ggtgtttgac accattcggc
     4021 agggggacta gtatcgctct ttcgacagtc accatggcgc tccagtccaa gtggcagcag
     4081 ctgcccaccg aggcgatcaa ccccgcaacc ctcggcatcg acaagctctc tcccgccggc
     4141 atcatcgacc tgatgctcga cgaggatcgc cgcatcatca gcgccgtcgc ccgcgaaaag
     4201 gaacggatcg ccctcggcgt cgagatgctg gcgcaggccg tgcggcgcgg cgggcggatc
     4261 gtcttcgttg gcgccggcac cagcggccgg ctcggggtgc tcgaggccgc ggagatgccg
     4321 ccctccttca gcacgagccc gtcgctcgtg caggcggtca tcgccggcgg gaagaaggcg
     4381 attacccgcg acgtcgaggg ggccgaggac gactacgagg ccggcgcgcg cgccctcgcg
     4441 cggctgcgcc cgggggcgaa ggacgtcgtg atcggcgtct cggcgagcgg catgaccccc
     4501 ttcgtgcggg gggcgctcag ccgcgcccgg aaggcggggg cgcgcatcgt cttcgtcacc
     4561 tgcttccccg gcaccgagct gcaaaccttc gtggacctga ccatcgcccc cgcggtgggc
     4621 cccgaggtga tcgccggctc gacgcggctg aaggcgggta ccgccaccaa gatcgtcctg
     4681 aacatgctca ccacgggcgc catggtccgc acgggcaaga cctacggcaa cctgatggtg
     4741 gacctgaaga ccgggtcgga gaagctcaag gaccgcgccc gccggatcct caccttcgtc
     4801 agcggcatcg actacgacac ggccgaccgc ctcttgaagc gcgcgcactg gaacgtgaag
     4861 gccgccatcg tgatgcagaa aaccggcctt cccttccgcc aggcgctctc gaaactgcgt
     4921 caggccgaca gcagcgtgcg cgcggcgatc gacgaggatg ccgagccccg gctgcgccag
     4981 ctcctcgggt cgcgcgcggg cgacccggac aaggagaagg agcgggagaa ggaaaagtag
     5041 cccgcccgcg cgatccctat gcacttgccg cttcgaccct cggttgccgt gctcgtcgcc
     5101 tgcctcgcgg ggctgacggc cgccgccccc ctggcgcagg cgcccgcgcc gcgggccacg
     5161 cgggccgaga tccgcgcgct gtgggtcgcc cgcacgtcgc tcacctcccc gcgcgccgtc
     5221 gaagagatgg tgtcgtcggc gcgggcggca ggcttcaaca ccttattagt acaggtgcgc
     5281 gggcgcggtg acgcgtactt cgccgacggc atggagcctc gggccgcgac gctcgcctcg
     5341 cagcccgagt cgttcgaccc cctggcgagg acgctcgcgc tcgcccgccc cctgggcctg
     5401 cacgtccacg catgggtgaa cgtcaaccta gtgtcgagcg ccgccgacct gccgtcttcc
     5461 cgcgagcacg tcgtctaccg gcacccggag tggctgatgg tcccgcggga gatcgcccgc
     5521 gacatgagcc tcctgaaccc gaagagcctg ctctacctgg acaagctggc gcgctgggcg
     5581 cgctcgcagc ccggcgagat cgaaggcctg tacctctcgc ccatccccgc gggggcggcc
     5641 gaccgcaccg tgcaggtgat cgcggacctg gtgacgcgct atcccgtgga cggcctgcac
     5701 ctcgactacg tccgctaccc gagcggcgcc ttcgactaca gccgggaggc cctcgaggcg
     5761 ttccgcgact cgctgctcga ggggctcgac gcgcgggagc gccagcggcg cggacaggcg
     5821 atcggcgccg acatcgtccg ctgggccgag cggcacccgg ccgagtggga ggcgttcagg
     5881 cgcgcgcggc tcaccgcgct ggtgtcgcgc atccgcgaaa gcctcgcgag gcgacgtccc
     5941 ggggcgctgc tgtcggcggc cgtctacccc gatgcggcgc gggccgcctc ggagcggctc
     6001 caggactgga gcggctggat cgatcgccgg ctgctcgacg tcgtgtgccc gatggcctac
     6061 gccaccgacg cggacgcctt caccgagcag ctcacggcgg cgaagcgggc ggcgcgcgac
     6121 ctgccggtgt gggccggcat cggcgcctac cgcctgtcca ccgagcagac catcgaaaac
     6181 atccgcatcg cgcggaggct cggcgcctcc ggcgtcgtcc tcttctcgta cgacagcctc
     6241 gtcgcctcgc ccaagggacc gggctacctc tcgcaggtga gccgcgcggt cttcgggggg
     6301 tagcgccgcg agggccatca cgggcgcacg cacatgccgc gcgtagccgg ggcagcagga
     6361 ttcagccggg cgaagggcgt gctggacgag gccgtcagcc gccgggtgtt cccgggtgcg
     6421 gccgcggagg ctggcgggcc cggcggcccg gcctggcagt acgccaccgg gacgctgtcc
     6481 tacgacgcct cgtcgccccc cgccaccatc gacaccgtct acgacctcgc gtcgctgacg
     6541 aaggtcctgt gcaccacgac cctcgtaatg cgccaggctc acagcggccg cctccccctc
     6601 gagacgccgc tctccgcgct gctccccgcc tggcgcggcg gcggccgcga cgtggtgacg
     6661 ctcgccgatc tcctcgcgca cgcctccggg ctgccggcgt ggcttccgct ctacgaaacg
     6721 tgtgccggcc gggcacagtt cgagcgggca attgccgcgg tgccgctcgc cggcccgccc
     6781 cggtcggtct ccgtgtacag cgatctcggc ttcatgctcc tgggcttcgt cctcgaagac
     6841 gtcggcggca cgagcctggc caggcagttc gaggcactgg aggccgatgg gcttggcttc
     6901 ggcgtcgcgc ccggcgaccg cgcgcgaact gcgccgaccc agcaggacga gtggcgggga
     6961 cggctgctgc ggggcgaggt ggacgatcgg aacgcgcacg cactcggcgg cacggcgggc
     7021 cacgcggggc tgttcgggac gattggcggc gtggggatgc tcgcgcgccg cctgatgcag
     7081 ggcctggagg gcgaggaagc ggccctgggc gtgacgcccg acacgctacg ggctttcgtc
     7141 acgcgcgtcg cgatcccagg cagctcgcgc gccctcggct gggacacgat gcttcccacc
     7201 tcgtcgtgcg ggtcacgaat gtcgccgcgc gcgttcgggc acaccgggtt caccggcacg
     7261 tcgctgtgga tcgaccccga gaagcggttc tacgcggtgc tgctctcgaa ccggattcat
     7321 ccaaagccgg gcgatccggc tgagatcctc gccgtgcgac aggccttcca cgacgcgctg
     7381 gcaggctagc gccggcagac tcccgatttc actgctgagg gttgccgccg gttggcggcc
     7441 ggacgccgcc cgggcggttg ccgggggcgc cgggggtgcc gggggtgccg aacccggggc
     7501 ggcccggcat ctgcccgggc ctggcccctg ggcggggctg gccgggcctc atgcccgggc
     7561 ggggctgtcc gggaccgaac agcgacggcg tgtagacgaa ctgccactcg ctgtagcggt
     7621 cgcgaccgtt gtacaagcgg atggacttct cggtgctctt gctggtgacc ccgatgacgc
     7681 cgccgcgcgg ccccacggtc cccgagagcc ccccggcggg cctgttggcg aacgccgagg
     7741 tccggggcgc ctgggcctgg tcgtcgcggc gggcctgatc gcgcgccacg ccgagctgcg
     7801 agccctgctc ccgccgaacg cccgagagcc cttcgccgcg cccctgctgc ccgaagcccg
     7861 gttgccggcc gggcaccatc ggctgcccca tcgacgcctg gtagagcacc tggaactcgc
     7921 cgtcggggac catcgggtcc ttgtatttct tgcgcaggaa cttctgctgg acgagcgtgt
     7981 cgatatctgg cgggtacgca gcggcgtact tccgctggaa gagcccgatg ctgcgggcgt
     8041 actgctgccc gcggaagatc agctcctcct ccttctcgcg ctgcaccatc tgccgccacg
     8101 ccggcatcgc gaccatcagc atgatggaca tcacgccgag cgcgacgagc atcgcggcca
     8161 tcgcgtagcc cgactcggac cgcggcgctt gaccctgcaa ccccatgtga ttacactgct
     8221 tgtcgactcc aatgatacca gacgagcgcc cgcaccgtgt ccctcggtcg gccggccggc
     8281 gcgtgtgccg ggcagccgtt ctgatcgtcc tgtcgtcggt gctggcgatc caggcctgcg
     8341 acaaggtcgc gctggtggca cccaccggca cgacgatcac gctgtacacc aactcgcagt
     8401 tcctcgccgt gaacgggacg gccgacatca ccgccagcgt gatcgaggcg aacggctacc
     8461 ccgtgcagaa cggcacgacc gtgacgttca cgacctcgct cggcaccgtc gagcccagtg
     8521 acgcgaagac gcacgacggc aaggccaccg taaagctgct gatcggcacg gccgccgtgg
     8581 gccaggtgct cgtgtcggcg actccgccca cggttccggc caccggcggc acggtgcaga
     8641 tcgtcgcaac ggtccaggat accggcggga acaacctgct gggggtgccg gtcaacttca
     8701 ccaccgactt cgggacgctc gtccccgaca ccgcggtcag cgactcgaac ggcaatgcgc
     8761 gcaccgtgct caccacttcg cagagcgcga cggtgaccgc gacggccggg gcgaagaacg
     8821 ccacggcgaa ggtcaccgca ctcgcggtgc cgacgatcac aatcacgccg ccgagtacga
     8881 cgccgagcgt cggggtgccg gcctcgttca caatcaacgt caccgcgggc gccaattcga
     8941 gcccgatcac cgacgtcacc gttgacttcg gcgaagggcg cccggtttcg ctcggtgccg
     9001 tcaatggcag tatcacggtc tctcacacgt acgacgtggc ggacgactac acggtgaccg
     9061 tcacggcgcg ggacgccacg ggcgtcacgg cca
//
LOCUS       DAOPQX010000125         8957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8760_length_8957_cov_5.140418, whole genome shotgun sequence.
ACCESSION   DAOPQX010000125 DAOPQX010000000
VERSION     DAOPQX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8760_length_8957_cov_5.140418"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKK95_08345"
     CDS             <1..291
                     /locus_tag="PKK95_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18262.1"
                     /translation="RDTIWPACSSVEVTYQHGYASLAESPYGDLVKGAVMTLAAEHAK
                     PSTIPARATSISTDLGNYRISQADATGKTGIPDVDAIIGLVGAAKPATGAGS"
     gene            288..719
                     /locus_tag="PKK95_08350"
     CDS             288..719
                     /locus_tag="PKK95_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18263.1"
                     /translation="MIEQLNLWAAQDALLDALKDQAAFDVDGAGEVALGLGFPAEIQP
                     EHVWIGGEAEGDLEAELTGPRPSAEIFRMPLFIFVQAADYEEARSRVKALAAACESAL
                     ETATFAAVVPSWRIPRYRLDAGTDGSNIQLCLELPVECRCW"
     gene            741..953
                     /locus_tag="PKK95_08355"
     CDS             741..953
                     /locus_tag="PKK95_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18264.1"
                     /translation="MAKRVRKYEILAPVSGSFTGRSGRVHYRFEPGIVRESDVDPDVL
                     SVLIATGKAVPVSTRAAKAAGSEESR"
     gene            950..2266
                     /locus_tag="PKK95_08360"
     CDS             950..2266
                     /locus_tag="PKK95_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18265.1"
                     /translation="MNLKLSAAGFAIQSAKGAAKAQPAWWGPVGGGGLVSFELEQTED
                     ELTSAQVGAIGEYRAAVAIAADYESRMWPASIGALLYAVLGDIQTSEGTPAVKAVLTT
                     ALTGVNNDLTFLAKTAGAAGNDITVALVDPSGNDQDLAVTVSDNAISVSLATGSGGLI
                     TSTALDVRNAIRAKAEAAALVEASVAPGNDGSGVVTALAAANLAGGADASGTGASTHV
                     ITPDASLPWCTVFGAKDTERKAASDCKLDSLKIEWEGNNPVKVTPTWAGMGVAWSDAA
                     YVPVLDESLLDYLKGIHLTAVLDLDGSGYDGGAEIKSGSVEIKRNIAGDTKSGQLEPS
                     SLDEGSFECDVELVVRVPDLLPVRLLLTGAVDGDSVSADVPYGEFSLTFADSPDSVAL
                     AATRVAWKTSEPEASPAGGPAELTLQGRCYGNPAITATVINAVASY"
     gene            2299..2697
                     /locus_tag="PKK95_08365"
     CDS             2299..2697
                     /locus_tag="PKK95_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18266.1"
                     /translation="MSKGARLLEVIYVDTEEPVNVLIGLGDSIRAAEWAEAEYPYPAK
                     PDLPADLSPAEVDFRIAEYRRERLDVDAKREWRSGLYAVFLAAQRAKLRGSELGWLEW
                     LSLVTIPQDDEPAPAEEPSAGESDAPQSAS"
     gene            2703..2882
                     /locus_tag="PKK95_08370"
     CDS             2703..2882
                     /locus_tag="PKK95_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18267.1"
                     /translation="MALDTLQPIGHLCDLLGISRCRPGVFDAMVDLLYGDESRGDAAR
                     EARLADLGRRLTGRR"
     gene            2884..3396
                     /locus_tag="PKK95_08375"
     CDS             2884..3396
                     /locus_tag="PKK95_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18268.1"
                     /translation="MPAPVYSTDVDVAGVSATRNLLRQMEPDLLRRLDRRLNVVANRV
                     KSVAQAEFERTGATGTAYRVRTRTRRGSFVKSIATAPGSVSRGQKWSSEPGALASIFE
                     LANAVRDAQPENVRRTQSLIETLNAKYYSPGRFLWSAWDAEGDRAQADVEDAVREAEI
                     EYTRRMEAVR"
     gene            3455..5767
                     /locus_tag="PKK95_08380"
     CDS             3455..5767
                     /locus_tag="PKK95_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HOC18269.1"
                     /translation="MRDLGRLQKQAELAGAGTAAGLLGASRSITSFGAGAAAAGMTLT
                     KGFTLPVAAIGGLAINESMKFSSAFAGVRKTIDATEPEFAKLRQGIRDMAKEIPASRE
                     AIAGVAEAAGQLGIENENVLSFTRTMIDMGESTNLASDEAATALARFANITGMEQTNF
                     DRLGSSIVALGNNMATTEAEVVNFGMRIAGAGAQVGMSEADIMGLSAAFSSVGISAEA
                     GGTALSKTMINIEASVQKGGEQLRLWAETAGMSAAEFKQAWEKDSAGALTAVVKGLGE
                     MEKQGGSTLGQLEKLGITEVRQRDALLRAAGAGDLLSDALKISGDAWQENGALAEEAG
                     RRYETPESRMAIMKNRASDMGITIGDELTPSFLRLMEDATRLAERFGNLTDGQQDFII
                     KAALVGAAIGPVLVIVGRLAQGVGAAVGVAGKLSLAFGKGGKAAPGWARGIAGATKGL
                     VSFVKQGALAIASLARQAASWVAETAAKAAATAATVAHTAVTKASAAAQWLLNAALSA
                     NPIGLVVIAIAGLVAALVVAYKKSETFRNIVNAAWGAIKTAAVAVFGFVANYIKTWVG
                     IIRAVLGKVKAVADSVVRFFGSMRDGIVSAFNRVTGFLRTVAGKMKSAIGNPGKILYE
                     VGKDIVAGLRNGISAAWHWVTDKLKGLIGGLSKAAKKVLGIGSPSRVFAAIGKDVAAG
                     LAIGIDGGKDAVRRAALGLAGATLPGFGAAGGYAVAAGAGGRSVVLHVAPGAVQINFG
                     SSPSGSVSQGTVDAAVDRAFRRLAAELSRR"
     gene            5772..7379
                     /locus_tag="PKK95_08385"
     CDS             5772..7379
                     /locus_tag="PKK95_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18270.1"
                     /translation="MPKVTVRPTKQQPLYKRIAQVGSTGAAAAVADQSDATYIRRSVN
                     GAPMARFLLAAPAIPADNDIATVVPGARLRQPTSNPPKLVSLAMSVPGTGKPKNKLLP
                     IVNGPSVRAGSGTSPYTFETPAAQGRMAGPRGPWADWLTRLAVRVNDGHKASDSNRAY
                     IYEVFADVYYAARPTVALATDPASPVTTTSYPELGATFSALVESWQDNEGEPARTEVA
                     YELRVFSQAQYEAAGFDPDLSTPLWETQGLTAPLDYIDGVTLSSEEAAETPDVPLPNG
                     AYRVYARGRRYFEAAQFGAWTYLALMVNVEPCPAPTLTATLDDANQRVVLEVTPNAKA
                     GATDPLTTIERSADAGATWEPVRGATSLAGTFAVKATLYDYEAPRGVALTYRAATEAT
                     ISDQQLVSDYATAAVVGTLSSSDWNLKCPLDPSLNLLDANVNADPEWTKEEEAATFRP
                     VGRTYPVVVSMALGGADGSLTVSTNTDAAWTALEALRDYAGTLLLESPYGWARYVRIL
                     SRSWAEMGAPEAARRRVTFSFLEVEAP"
     gene            7379..8482
                     /locus_tag="PKK95_08390"
     CDS             7379..8482
                     /locus_tag="PKK95_08390"
                     /inference="COORDINATES: protein motif:HMM:NF027781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5047 domain-containing protein"
                     /protein_id="HOC18271.1"
                     /translation="MYPVSDAFREAIVSCDQRAVVRAVVELDGSELGVLPLTGGAVDC
                     DGTRDGALRSLSLTVSPHPDAFDWLATAGAEVVVARGLRLPDGTEELVPLGVFVMDAD
                     LEEAEDGTVTVSAGDRSRRISRARWTDPYVVPAGTAVGAALADLLRACWPGCPVGSTL
                     QAADKTTGAKLAYLDGADSDPWKDARDLAASAGLDLYFDGDGVAQVRDTPDPESDPVC
                     WTYRSGDEAVVLGRTRKAMLTELYNGVVVTAEGSGVATPRRGEAWDEDPNSPTYAYGP
                     MGRVPRFYSSPLLTTQAEVDSAAQTMLARVKRPIEQASFSLLPNPAHEAFDVVEFVDA
                     DGVSSRFMFDVVSVPLDSSGALTATARETMVIT"
     gene            8479..>8957
                     /locus_tag="PKK95_08395"
     CDS             8479..>8957
                     /locus_tag="PKK95_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18272.1"
                     /translation="MKELAEALKAKAGLRMRQGVVSAVDGSTVSVLIGGSTVAVDGVH
                     HLNSVAPQVGEVVWIASDGADLWILGTHGDPPAIDPSRLPDYATYFASAAPEGAPATP
                     TNVLGTALLNGLLVKWDRTAYDPWLTWEIQLSDDDWVSWDTFTTATQPVVVPLPPGGP
                     "
BASE COUNT         1317 a         3219 c         3112 g         1309 t
ORIGIN      
        1 cgggacacga tctggccggc gtgttcctct gtcgaggtta cctaccagca cggctacgcg
       61 agcctcgccg agtcgcccta cggggatctc gtgaagggtg ccgtcatgac gctggccgcc
      121 gagcacgcga agccgtccac catcccggcg cgggccacgt cgatctcgac cgacctgggc
      181 aactatcgga tctcgcaggc cgacgcgacc ggcaagaccg gcatcccgga cgtcgacgcc
      241 atcattggcc tggtcggcgc cgcgaagccc gccacggggg ccggctcgtg atcgagcagc
      301 tcaacctgtg ggcggctcag gacgcgctcc tggatgcgct caaagaccag gccgccttcg
      361 acgtggacgg cgccggcgag gtcgccctgg ggctcggctt cccggccgag atccagcccg
      421 agcacgtctg gatcggcggc gaagccgagg gcgatctcga ggccgagctc accgggccgc
      481 ggccgtcggc ggagatcttc cgcatgcccc tgttcatatt cgtgcaggcg gccgactacg
      541 aggaggcgcg ctcacgcgtc aaggccctgg cggctgcgtg cgagtcggcg ctcgagacgg
      601 cgaccttcgc tgccgtcgtt ccctcgtggc gcatcccgcg gtaccggctc gacgccggca
      661 ccgacggctc caatattcaa ctctgcctcg agctcccggt cgagtgccgg tgttggtgac
      721 gctgaaagga caatgaggct atggccaagc gcgttcgcaa gtacgagatc ctcgcgcccg
      781 tcagcggctc gttcaccggc cgctcgggtc gcgttcacta ccgcttcgag cccggcatcg
      841 tgcgcgagtc ggacgtggac ccggacgtgc tctcggtcct catcgccacg ggcaaggccg
      901 tgccggtgtc caccagggcg gcgaaggccg caggaagtga ggaatcccga tgaacctgaa
      961 actgtccgct gccggcttcg cgatccagtc ggcgaagggt gccgccaaag cccagccggc
     1021 ctggtggggt ccggtcggcg gtggtgggct cgtgtcgttc gagctcgagc agaccgagga
     1081 cgagctcacg tcggcgcagg tcggcgccat cggcgagtac cgcgcggcgg tcgccatcgc
     1141 cgcagactac gagagccgca tgtggccggc gtcgatcggc gctctgctgt acgccgtgct
     1201 cggcgacatc cagacgagtg agggcactcc tgccgtcaag gccgtgctca ccacggcgct
     1261 caccggcgtc aacaacgact tgacgttcct ggccaagacc gccggcgctg ccggcaacga
     1321 catcaccgtg gcgctggtag atccttcggg caacgaccag gatctcgcgg tcaccgtcag
     1381 cgacaacgcc atttctgtga gcctggcgac cggctcgggt ggcctcatca cgtcgaccgc
     1441 gctcgacgtg cgcaacgcca tccgggcgaa ggccgaggcc gccgctctcg tggaggcgtc
     1501 tgtcgctccc ggcaacgacg gcagtggcgt ggtgacggcg ctcgccgccg ccaacctggc
     1561 cggcggcgcc gacgcttccg ggacgggcgc gtcgacgcac gtcatcactc ccgatgcctc
     1621 gctcccgtgg tgcaccgtct tcggcgccaa ggacaccgag cgcaaggccg cgtcggactg
     1681 caagctcgat tcgctcaaga tcgagtggga gggcaacaac ccggtgaagg tcacgccgac
     1741 ctgggccggc atgggcgtcg cgtggtcgga cgccgcctac gtcccggtgc tcgatgagtc
     1801 cctgctcgac tacctcaagg gtatccatct caccgcggtg ctcgacctgg acggctcggg
     1861 ctacgacggc ggcgccgaga tcaagtctgg ctcggtcgag atcaagcgca atatcgcggg
     1921 cgacaccaag tccgggcagc tcgagccgag cagcctcgat gagggctcgt tcgagtgcga
     1981 cgtcgagctc gtcgtgcgcg tgcccgacct gctgcccgtg cggctgctcc tgaccggcgc
     2041 cgtcgacggc gactccgtgt cggccgatgt gccctacggc gagttctctc tgacgttcgc
     2101 cgattctccc gacagcgtgg cgctcgccgc cacgcgcgtc gcctggaaga cgtcggagcc
     2161 cgaggccagt cccgccggtg gtcccgccga gctcactctg cagggccgct gctacggcaa
     2221 ccccgccatc accgcaaccg tcatcaacgc tgtcgcctcg tactgagcct ggcgacggcc
     2281 gacccgaggg ggtaatccat gagcaagggc gcgaggctgc tcgaggtcat ctacgtcgac
     2341 accgaggagc cggtgaacgt cctcatcggg ctcggcgact ccatccgtgc tgccgagtgg
     2401 gcggaggccg agtacccgta cccggcgaag cccgatctcc cggcggatct gtcgcccgcg
     2461 gaggtcgact tccgcatcgc cgagtaccgc cgcgagcggc tcgacgtcga cgccaagcgc
     2521 gagtggcgct cgggcctgta cgccgtgttc ctggccgccc agcgcgcgaa gctccgcggt
     2581 tcggagctcg gctggctcga gtggctgagt ctcgtgacca tcccgcagga cgacgagccc
     2641 gcgccggccg aggagccgtc cgcgggggaa tccgacgcgc cacagagcgc cagttagctc
     2701 gtgtggcgct ggacaccctg cagccgatcg gccacctgtg cgacctgctc ggcatctccc
     2761 ggtgccggcc gggcgtcttc gacgccatgg tcgatctcct ctatggcgat gagtcgcgcg
     2821 gcgacgcagc ccgcgaagct cgcctcgccg acctgggccg gcgactgacc gggaggcgct
     2881 aaggtgcccg cacccgtcta ctcgaccgac gtcgacgtcg ccggcgtctc cgccacgcgc
     2941 aacctcttgc gccagatgga gccggatctg ctcaggcggc tcgaccggcg gctgaacgtc
     3001 gtggcgaacc gcgtcaagtc ggtcgcccag gccgagttcg agcggacggg cgcgacgggc
     3061 accgcctacc gggtccgcac gcgcacgcgc cgcggatcct tcgtcaagtc gatcgccacg
     3121 gcgcccggct cggtttcgcg gggccagaag tggtcgagcg agccgggcgc gctggcgtcc
     3181 atcttcgagc tcgctaacgc cgtgcgcgat gcccagccgg agaacgtgcg gcgcacgcag
     3241 agcctcatcg agacgctcaa cgccaagtat tactcgccgg gccggttctt gtggtccgcc
     3301 tgggatgccg agggcgaccg ggctcaggcc gacgtcgagg acgccgttcg cgaggccgag
     3361 atcgagtaca cgcgccgcat ggaggcggtg aggtaacgtg gccgttgtca tccccatcgt
     3421 taccagctac aggggctccg gcgccaatgc cgccatgcgc gaccttggtc gcctgcagaa
     3481 gcaggccgag ctcgccggcg ccggcaccgc cgccggtctg ctcggcgctt cgcgctccat
     3541 caccagcttc ggcgccggcg cggccgccgc cggcatgacc ttgaccaagg gcttcacgct
     3601 gccggtcgcc gcgatcggcg gcctggctat caacgagtcg atgaagttct cgagcgcgtt
     3661 cgccggcgtg cgcaagacca tcgacgcgac cgagcccgag ttcgccaagc tgcgccaagg
     3721 catccgcgac atggccaagg agatcccggc gagccgcgag gccatcgccg gcgtcgccga
     3781 ggccgccggc cagcttggta tcgagaacga gaacgtcctc agcttcacgc gcacgatgat
     3841 cgacatgggc gagtccacga acctcgcgtc cgacgaggcc gcgacggcgc tcgcccgctt
     3901 cgccaacatc accggcatgg agcagacgaa cttcgaccgc ctgggctcct cgatcgtggc
     3961 gctgggcaac aacatggcga cgaccgaggc cgaggtcgtg aacttcggca tgaggatcgc
     4021 cggcgccggt gcccaggtcg ggatgagcga ggcggacatc atgggcctgt ctgctgcgtt
     4081 ctcctctgtc ggcatcagcg ccgaggccgg cggcacggcg ctctcgaaga ccatgatcaa
     4141 catcgaggcg tccgtgcaga agggcggcga gcagctccgg ctgtgggccg agaccgccgg
     4201 catgagcgcg gccgagttca agcaagcctg ggagaaggat tcggccggcg cgctcacggc
     4261 cgtcgtcaag ggtcttggcg agatggagaa gcagggcggc tcgacgctcg ggcagctcga
     4321 gaagctcggc atcaccgagg tccgccagcg cgacgcgctc ctgcgcgccg ccggcgccgg
     4381 cgacctgctc tccgacgctc tgaagatctc gggcgacgcc tggcaggaga acggcgcgct
     4441 cgccgaggag gccggccggc gctacgagac gcctgagtcg cgcatggcca tcatgaagaa
     4501 ccgcgccagc gatatgggca tcacgatcgg cgacgagctc acgccgtcgt tcctgcggct
     4561 catggaggac gcgacccggc tggcggagcg gttcggaaac ctcacggacg gccagcagga
     4621 cttcatcatc aaggccgccc ttgtgggcgc cgccatcggg ccggtgctcg tcatcgtcgg
     4681 gcggctcgcg cagggcgtcg gtgccgcggt gggcgtcgcc ggcaagctgt cgctcgcctt
     4741 cggcaagggc gggaaggcgg cgcccggctg ggcgcgcggc atcgcggggg ctaccaaggg
     4801 actcgtctcg ttcgtcaagc agggcgcgct cgccatcgca tcgctcgcgc ggcaggcagc
     4861 ctcgtgggtc gcagagacgg ccgcgaaggc cgccgcgacc gccgccacgg tggcgcacac
     4921 ggccgtcacc aaggcgtccg ccgcggcgca gtggctgctc aacgcggcgc tctccgccaa
     4981 cccgatcggg ctcgtcgtca tcgccatcgc cggcctggtg gccgcgctcg tggtcgccta
     5041 caagaagtcg gagacgttcc gcaatatcgt gaacgccgcc tggggagcca tcaagacggc
     5101 cgccgtcgcc gtcttcggct tcgtcgccaa ttacatcaag acgtgggttg gcatcatccg
     5161 ggcggtgctc gggaaggtca aggccgtggc cgactccgtc gtgcgcttct tcgggagcat
     5221 gcgcgacggc atcgtcagcg ccttcaaccg cgtgaccggc ttcctgcgca ccgtcgccgg
     5281 caagatgaag tcggccatcg gcaacccggg gaagatcctc tacgaggtcg gcaaggacat
     5341 cgtggccggg ctgcgcaacg gcatctccgc cgcctggcac tgggtcaccg acaaactcaa
     5401 ggggctcatc ggcgggctct ccaaggccgc gaagaaggtg ctcggcatcg gctcgccctc
     5461 gcgcgtcttc gccgccatcg gcaaggacgt cgccgccggg ctcgcgatcg gcatcgacgg
     5521 cggcaaggat gccgtgcgtc gggccgcgct agggctcgca ggagccactt tgccgggctt
     5581 tggcgccgca ggagggtacg cggtcgccgc gggggccggc gggcgctctg tcgtgctcca
     5641 cgtggctcct ggcgcagtgc agatcaactt cggctcgagc ccgtccggga gcgtgtcgca
     5701 ggggaccgtg gacgccgcgg tcgaccgcgc cttccgccgc ctggccgcag agctctcgag
     5761 gaggtgacgt cgtgcccaag gtgacggtgc ggccgaccaa gcagcagccg ctctacaagc
     5821 gcatcgccca ggtggggtcg acgggcgccg ccgctgccgt ggcggaccag agcgacgcca
     5881 cctacatccg ccgctcggtg aacggcgcgc cgatggcgcg cttcctgctg gcggcgccgg
     5941 cgatcccggc ggacaacgac atcgccacgg tcgtgccggg cgcgcgcctg cgccagccga
     6001 cgtccaaccc gccgaagctc gtgtcgctcg ccatgagcgt cccgggcacg ggcaagccga
     6061 agaacaagct gctgccgatc gtgaacgggc cgagcgtgcg cgccggctcg ggcacgagtc
     6121 catacacctt cgagacgccg gccgcccagg gccgcatggc gggtccgcgc gggccgtggg
     6181 cggattggct cacgcggctc gcggtgcggg tcaacgacgg gcacaaggcg agcgactcaa
     6241 accgtgcata tatatacgag gtgttcgcgg atgtgtacta cgccgcgcgg ccgaccgtcg
     6301 cgctggccac agacccggcg tcgccggtca cgaccacgtc ctacccggag ctcggcgcga
     6361 cgttctccgc cctcgtggag tcctggcagg acaacgaggg cgagcccgcg cgcaccgagg
     6421 tcgcttacga gctccgcgtc ttctcgcagg cgcagtacga ggcggccggc ttcgacccgg
     6481 atctctcgac gccgctgtgg gagacgcagg ggctcacggc tccgctcgac tacatcgacg
     6541 gcgtcacctt gtcctctgag gaggccgccg agactcccga cgtgccgctg cccaacgggg
     6601 cgtatcgcgt ctacgctcgc ggccggcgct acttcgaggc ggcgcagttc ggcgcctgga
     6661 cctacctcgc cttgatggtc aacgtcgagc cctgcccggc gccgaccctg accgccacgc
     6721 tcgacgacgc caaccagcgg gtcgtgctcg aggtcacgcc gaacgcgaag gccggcgcta
     6781 cggatccgct caccacgatc gagcgcagcg ccgacgccgg cgccacctgg gagccggtgc
     6841 gcggcgctac ctcgctcgcc ggcaccttcg ctgtcaaggc gacgctctac gactacgagg
     6901 cgccgcgcgg ggtggcgctc acctaccgcg ccgcgaccga ggcgacgatc tccgaccagc
     6961 agcttgtctc tgactacgcc acggctgccg tggtgggcac gctctcgagc tcggactgga
     7021 acctcaagtg cccgctcgac ccgtcgctca acctgctcga cgcgaacgtg aacgccgacc
     7081 cggagtggac caaggaggag gaggccgcga ccttccgccc ggtggggcgc acgtacccag
     7141 tggtcgtctc catggcgctc ggcggcgccg acggctccct gaccgtcagc accaacaccg
     7201 acgccgcatg gacggcgctc gaggcgctcc gcgactacgc cggcacgctc ctactcgagt
     7261 cgccttacgg ctgggcacgc tacgtgcgga tcctctcgcg gtcttgggct gagatgggcg
     7321 ctcccgaggc ggcgcgccgg cgcgtgacgt tctcatttct cgaggtcgag gctccctgat
     7381 gtacccggtg tctgacgcct tccgcgaagc gatcgtctcc tgcgaccagc gggccgtggt
     7441 ccgcgccgtg gtcgagctcg acggttccga gctcggcgtg ctgccgctca ccggcggggc
     7501 ggtcgactgc gacggcacgc gcgacggcgc gctccgctcc ctgtcgctca ccgtctcgcc
     7561 gcatcccgac gccttcgatt ggctggcgac cgccggcgcc gaggtcgtcg tcgcccgcgg
     7621 gcttcggctg cccgacggca ccgaggagct cgtgccgctc ggcgtgttcg tcatggacgc
     7681 ggatctcgag gaggccgagg acggcacggt cacggtgtct gccggcgacc gctcccggcg
     7741 catcagccgc gcccgctgga ccgaccccta cgtggtgccg gccggcaccg ccgtcggcgc
     7801 agcgttggca gatctcctgc gcgcctgctg gcctggctgc ccggtcggct cgaccctgca
     7861 ggcggccgac aagacgacgg gcgcgaagct cgcctacctc gacggcgccg actcggatcc
     7921 gtggaaggac gcgcgcgacc tggccgcgtc cgccggcctg gacctgtact tcgacggcga
     7981 cggcgtcgcc caggtgcgcg acactcccga cccggagtcc gacccggtgt gctggaccta
     8041 ccgctcgggc gatgaggccg tggtcctggg ccgcacgcgc aaggccatgc tgaccgagct
     8101 ctacaacggc gtcgtggtca cggccgaggg ctccggcgtt gctactcctc gccggggtga
     8161 ggcgtgggac gaggatccga actcaccgac ctatgcctac gggccgatgg gccgcgtgcc
     8221 gcgcttctac tcctcgccgc tgctcaccac gcaggccgag gtcgactcgg ccgctcagac
     8281 catgctggcg cgcgtcaagc gcccgatcga gcaggcgtcg ttctccctgc tacccaaccc
     8341 tgcgcacgag gcgttcgacg tcgtggagtt cgtggacgcc gacggcgtct cgagccgctt
     8401 catgttcgac gtcgtgtcgg tgccgctcga ctcctcgggc gcgctcacag ccacggcgcg
     8461 agaaacgatg gtgatcacgt gaaggaactc gccgaagctt tgaaggccaa ggccggcctg
     8521 cgcatgaggc agggcgtcgt gagcgcggtc gacggctcga ccgtgagcgt cctcatcggc
     8581 ggatccacag tcgcggtcga cggcgtgcac cacctgaaca gcgtggcgcc gcaggtcggc
     8641 gaggtcgtgt ggatcgcctc ggacggcgcc gacctgtgga tcctgggcac gcacggcgac
     8701 ccaccggcga tcgacccgag ccgcctgccc gattacgcga cctacttcgc ctcggccgct
     8761 cccgagggcg cgccggcgac tccgacgaac gtcctcggca cggcgctcct gaacgggctc
     8821 ctggtgaagt gggaccgcac cgcctacgat ccctggctca cctgggagat ccagctatcc
     8881 gacgacgatt gggtgtcgtg ggacaccttc acgaccgcca cgcaaccggt cgtcgtcccg
     8941 ctcccgcctg gcgggcc
//
LOCUS       DAOPQX010000126         8898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8830_length_8898_cov_4.416714, whole genome shotgun sequence.
ACCESSION   DAOPQX010000126 DAOPQX010000000
VERSION     DAOPQX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8830_length_8898_cov_4.416714"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..2426
                     /locus_tag="PKK95_08400"
     CDS             120..2426
                     /locus_tag="PKK95_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC18273.1"
                     /translation="MEFLNQLNPEQREAVLRVEGPLLILAGAGSGKTRVIAYRIAYLI
                     GGGHASPYEVVALTFTNKAAEEMRGRVATLLGDACEGAWVMTFHAMCARLLRREAPHV
                     GLSRDFVIYDSSDQVSAVKQVMRALNIDDGVLQPRSVLSRISHAKNQMKGPEDFEESY
                     NPRDAELGKIFRGYQSALAASNAVDFDDLLLKSLQLFEEHEDLRLKYARRFKFLLVDE
                     YQDTNRPQYLLLRRMAEAHRNLCVVGDPDQSIYKWRGADIHNILDFERDYADAFVVRL
                     ERNYRSTQVILDAASAVISRNVERKEKRLYTEEAGGDKLLYHQAADELEEADFIARTA
                     RRLAHSPAQSPMAVLYRTNAQSRVIEDAMRREGIPYRIIGSVRFYERKEIKDALSYLK
                     LLLNPDDDVSLRRVINVPARGIGKGVMDALAAFVPDTYPGDRAQASLPLGGARAANSL
                     WAKLRAGLDAGAFTGRAASSLRAFRGLIEGLSEVARTEPVSIVLGKVLDRTGYLNELR
                     EEKTPEAEGRVDNLMELVSAAKEYEARNANGNGDGGGGASLGGFVDQLSLLSDADESE
                     GAADARVLMMTLHSAKGLEFPVVVLAGMEENLFPHSRSSDDQAELEEERRLCYVGMTR
                     ARRRLLLTGAGRRRWYGEYRAQAPSRFLNEIPPELVDLDVAGVSGGHYQPSFSSAFER
                     RPNPYRRGPHATPTRVREEAPLYAYEDEDQSRPGHIEVGARVRHAQFGVGTVVSVQAA
                     GGDTKLVVRFASVGTKTLIARFANLEVV"
     gene            complement(2494..3213)
                     /locus_tag="PKK95_08405"
     CDS             complement(2494..3213)
                     /locus_tag="PKK95_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18274.1"
                     /translation="MHRPRYRWLFIIPILAALVLSSACGGNPEDPLIRKFFQASRMGD
                     NMTLANIATVSFDPSRDGMAQNVKVVSVTPEQTRVLQFKELEKARQEAVAAEAEFSKK
                     MKAYQDDNLDAINRVLKLEQTNGKPRGRDAEVQAAWTKWREEQKEYNRKVSEARTKLN
                     AERRVADLSVTDHDPTGYDGTEYTKEATVTANVRMPDGQTAEKTYTLTLQRVVLKDPQ
                     GGADIEGKWMITAIKEAGPAS"
     gene            complement(3225..5702)
                     /gene="gyrA"
                     /locus_tag="PKK95_08410"
     CDS             complement(3225..5702)
                     /gene="gyrA"
                     /locus_tag="PKK95_08410"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOC18275.1"
                     /translation="MDALTKLPVNIEDEMKRSYMDYAMSVIIGRALPDVRDGLKPAHR
                     RVLYGMRTMGLASNRAYRKCAKIVGEVMGNFHPHGDAAIYDTLVRLAQDFNMRAPLVD
                     GQGNFGSMDGDPPAAMRYTEARLKPLAEEMMADLDKETVDFVPNYDETTEEPTVLPAP
                     FPNLLVNGSVGIAVGMATNIPPHNLREVVDGAIWLVEQRQREQAGGEPPMSADAKLKA
                     LLKIVPGPDFPTAGLIVGRSGIVQAYRTGRGAITVRARATVEESAKGDRSSIVITEIP
                     YQVNKAKLVEKIAELARDKTIEGISDLRDESDRQGLRIVIELKRGEVADVVLNNLYKH
                     TPLQSTFGIIMLAIVGGRPVVLPLVEVLEQFVEFRREVVRRRTEYLLRKAEARAHILE
                     GLKIALDHLDEVIALIRAAKSPLEARDGLMTRFGLSQVQSQAILDMQLQRLTGLERQK
                     ILDELAEVLRQIERYRAILGSEALLMEIVVHELREVRDKYGDGRRTEIVEEEGEFRVE
                     DLIVDEDVAITVSNTGYIKRTAVTEYRTQRRGGLGRRGMSVREEDFVAHLFIASTHSY
                     ILIFSDKGRVYWLKVYEIPDVGPDGKGKAIANLVAMLPEEKIAALLPVREFEAGRHVV
                     MGTRRGVIKKTELTSFRNPRAAGIIAMGVEENDAVIAVQTSDGSGHIFIGTRDGMAIR
                     FHEQDVRPTGRGTYGVRGITLREGDEVVAMEVTNGGGTLLTVSENGYGKRTNLDEYRV
                     QSRGGLGIINIHTTDRNGRVVGIAYVRDEDELMLITQQGKVLRMGTRDVRTIGRATQG
                     VRLIGVEEDDRVVSIARLAERESDLNE"
     gene            complement(5830..8505)
                     /gene="gyrB"
                     /locus_tag="PKK95_08415"
     CDS             complement(5830..8505)
                     /gene="gyrB"
                     /locus_tag="PKK95_08415"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOC18276.1"
                     /translation="MSDTNASGHGFEETVSPQATENSAGTPEVAPSNGSGAPDEEQLH
                     DLGVDEYGADKIKVLEGLEAVRKRPAMYIGSTGPLGLHHLVYEVVDNSIDEALAGFCD
                     TINVTIHLDNSVTVVDNGRGIPVDMHESGRSAAEVVMTVLHAGGKFDNNSYKVSGGLH
                     GVGVSVVNALSETLDLEIWRNGLVYHQVYERGQPQADLEITGKTKKRGTKIRFLPDRG
                     VFETIEFSFDTLAQRLRELAFLNPGVAITLEDDRTDGKGHRFQYDGGIREFVTHLNKN
                     KSGVTDKPIYMRGERDGIDAEIALQWNDGYTETIYTFANNINTHEGGTHLSGFRAALT
                     RTVNAYAAKNNLTKDLKESIGGDDIREGLTAVISVKIPRPQFEGQTKTKLGNTEVKGI
                     VEAIVNDKLGAFLEENPAVARRIVMKSVDAARAREAARKARDLVRRKGALENSSLPGK
                     LADCQERDPALSELYIVEGESAGGSAKQGRDRKFQAILPLRGKILNVEKARFDKMLSS
                     EEIKVMISALGCGVGPEDFDVEKLRYHRIIIMTDADVDGSHIRTLLLTFFYRQLPELI
                     ERGYVYIAQPPLFRAKRGKAEHYIKDERELEHWLMRRAIESRVLRLKDGRELAGDALE
                     KLLHDLIAFRRYLQVVERRGYQFDVLKAVMDRDAHDKSFFADAEGLQQLAEELSSATR
                     TVTVQPDEEHNLSSLHVEDRSNGYPRHYQLDIDFLTGVDYRTLASSYKEIQGLELPVV
                     VAATSAPAVEVDEHDPGLPTASANDTRIGGGAIDPATKLAAEPAPEPRESAGRSSKDP
                     ETVTLTTLDQVVEFFVTAGKRGVAVNRYKGLGEMNPEQLWSTTMRPESRTLLQVRAED
                     HTEADLMFTTLMGDQVEPRRKFIEDNALDVRNLDI"
     gene            complement(8529..>8898)
                     /locus_tag="PKK95_08420"
     CDS             complement(8529..>8898)
                     /locus_tag="PKK95_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18277.1"
                     /translation="VPKGNDKVIEFERERLAAALKRVGQLSPERSRAVKLAIEPGKVE
                     VSSQSPEYGEAKEILGVDDYAGEALSIGFNGQYVQQFLEVVETDRVALELKDEVSQAV
                     MKPVGQEGFQYTYVLMPMRF"
BASE COUNT         1249 a         3172 c         2905 g         1572 t
ORIGIN      
        1 gcgacgtgga ccagccgcgc aacctggcga agtcggtcac ggtagaatag ggggaacccc
       61 cgcgcgtttg cgtgaagact tcggccgggc ggccacaagg gccgcccctg cgctacctca
      121 tggaattcct gaaccagctc aaccccgagc agcgcgaggc ggtgctgcgg gtggagggac
      181 ccctgctcat cctggccggc gccgggtcgg gcaagacgcg cgtcatcgcg taccgcatcg
      241 cgtacctcat cggcggcggc cacgccagcc cctacgaggt ggtcgcgctg accttcacca
      301 acaaggccgc cgaggagatg cgggggcgcg tcgccacgct gctcggcgac gcgtgcgagg
      361 gcgcgtgggt gatgaccttc cacgcgatgt gcgcccgcct gcttcgccgc gaggcgccgc
      421 acgtcggcct ctcgcgcgac ttcgtgatct acgactcgtc cgaccaggtc tcggccgtca
      481 agcaggtgat gcgcgcgctc aacatcgacg acggggtgct gcagccgcgg tcggtgctca
      541 gtcgcatcag ccacgccaag aaccagatga aggggccgga ggacttcgag gagagctata
      601 acccgcgcga cgccgagctg gggaagatct tccgcggcta ccagtcggcg ctcgccgcga
      661 gcaacgccgt cgatttcgac gacctgctcc tcaagtcgct gcagctgttc gaggagcacg
      721 aggacctgcg gctcaagtac gcccggcgct tcaagttcct gctggtggac gagtaccagg
      781 acaccaaccg cccgcagtac ctgctgctgc gccgcatggc cgaggcccac cgcaacctgt
      841 gcgtcgtcgg cgaccccgac cagtcgatct acaagtggcg cggcgccgac atccacaaca
      901 tcctcgactt cgagcgcgac tacgccgacg cgttcgtggt gaggctcgag cgcaactacc
      961 gctcgaccca ggtgatcctc gacgccgcct ccgccgtcat cagccggaac gtcgagcgca
     1021 aggagaagcg gctctacacc gaggaggccg gcggcgacaa gctgctgtac caccaggccg
     1081 ccgacgagct cgaggaggcc gacttcatcg cccgcaccgc ccgccgcctg gcccactcgc
     1141 cggcgcagtc gccgatggcg gtgctctacc gcaccaacgc gcagtcgcgg gtcatcgaag
     1201 acgcgatgcg ccgcgagggg atcccctacc ggatcatcgg cagcgtccgg ttctacgagc
     1261 gcaaggagat caaggacgcc ctctcgtatc tgaagctcct cctcaatccc gacgacgacg
     1321 tgagtttgcg gcgggtgatc aacgtccccg cgcgggggat tggcaagggg gtgatggacg
     1381 cgctcgcggc gttcgtcccc gacacctacc ccggcgaccg cgcgcaggcc tcgctcccgc
     1441 tcgggggggc gcgcgcggcg aactcgctgt gggcgaagct gcgcgcgggc ctcgacgccg
     1501 gcgccttcac cgggcgcgcg gcctcgtcgc tgcgcgcctt ccgcggcctg atcgaggggc
     1561 tgtcggaggt ggcgcgcacc gagccggtgt cgatcgtgct cggcaaggtg ctcgatcgca
     1621 ccggctacct gaacgagctg cgcgaggaga agacgcccga ggccgagggg cgcgtcgaca
     1681 acctgatgga gctggtgtcg gcggccaagg agtacgaggc gcgaaacgcg aacggcaacg
     1741 gggacggcgg cggcggggcg tcgctcggcg gcttcgtgga ccagctctcg ctgctgtcgg
     1801 acgccgacga gtcggaggga gcggccgacg cacgcgtgct gatgatgacg ctccacagcg
     1861 ccaagggcct cgagttcccg gtggtggtcc tcgcgggcat ggaggaaaac ctcttccccc
     1921 actcgcgctc ctccgacgac caggccgagc tcgaggagga gaggcgcctc tgctacgtgg
     1981 ggatgacgcg ggcccggagg cggctgctgc tgacaggcgc gggccggcgg cgctggtacg
     2041 gcgagtaccg cgcacaggcc ccgtcgcggt tcctgaatga gatcccgccg gagctggtgg
     2101 atctcgacgt ggctggcgtc tcgggcggcc actaccagcc gtcgttctcg tcggcgttcg
     2161 agcggcggcc caacccgtac cgacggggcc cgcacgccac ccccacccgc gtgcgcgaag
     2221 aggcgcccct ctacgcctac gaggacgagg accagtcgcg cccgggccac atcgaggtgg
     2281 gtgcccgcgt ccgccacgcc cagttcggcg tggggaccgt ggtgagcgtc caggcggccg
     2341 ggggcgacac caagctggtg gtccgcttcg cgtcggtggg gacgaagacg ctgatcgcga
     2401 ggttcgcgaa tctggaggta gtgtagtcaa aaaaaagggc gcggcttgta caccgcgccc
     2461 ttttcgtcgt acccgaatcc cgattcctga atcctaggag gccggacccg cctccttgat
     2521 cgccgtgatc atccacttgc cctcgatgtc ggcgccgccc tgcggatcct tgaggaccac
     2581 gcgctgcagc gtcagcgtgt aggtcttctc ggcggtctgg ccgtccggca tccggacgtt
     2641 cgcggtcacg gtcgcttcct tcgtgtactc cgtgccgtcg tacccggtgg ggtcgtggtc
     2701 ggtcacgctc aggtcggcca cccgccgctc ggcgttcagc ttcgtccggg cctccgagac
     2761 cttccggttg tactccttct gctcctcgcg ccacttcgtc caggcggcct gcacctcggc
     2821 gtccctgccc cgcggcttcc cgttggtctg ctcgagcttc agcacccggt tgatggcgtc
     2881 caggttgtcg tcctggtagg ccttcatctt cttcgagaac tcggcctcgg ccgccaccgc
     2941 ctcctgccgc gccttctcga gctccttgaa ctggagcacc cgggtctgct ccggggtcac
     3001 cgagaccacc ttgacgttct gggccatccc gtcgcgcgac gggtcgaacg acacggtggc
     3061 gatgttcgcc agcgtcatgt tgtcgcccat ccgcgaggcc tggaagaact tgcggatcag
     3121 cggatcctcg gggttccccc cgcacgcgct cgacaggacc agggccgcca ggatggggat
     3181 gatgaataac cagcgataac gcggccgatg catgccagcc tcctttactc gttcaggtcc
     3241 gattctcttt cggcgagtcg ggcgattgac acgacccggt cgtcctcttc caccccgatc
     3301 aagcgcacgc cctgggtagc ccgtccaatc gtccggacgt cgcgggtgcc catgcgcaga
     3361 accttcccct gctgcgtgat gagcatcagc tcgtcctcgt cccggacgta cgcaattccg
     3421 acaactcggc cgttccggtc ggtcgtgtgg atgttgatga tacccaaacc gcccctggac
     3481 tgcacgcgat attcgtccag gttggttcgc ttgccgtagc cgttctcgga aaccgtcagc
     3541 agggtccccc cgccgttggt cacctccatg gccaccacct cgtcgccctc gcgcagggtg
     3601 atgccccgaa cgccgtaagt gcctctcccc gttggcctca cgtcctgctc gtggaaccgg
     3661 atggccatgc cgtcccgggt gccgatgaag atgtggccgc tgccgtcgct ggtctgcacg
     3721 gcgatgaccg cgtcgttctc ctcgaccccc atcgcgatga tcccggccgc ccgcgggttc
     3781 cggaacgagg tcagctcggt cttcttgatc accccgcggc gggtgcccat caccacgtgg
     3841 cggcccgcct cgaactcccg caccggcagc agcgcggcaa tcttctcctc gggcagcatc
     3901 gcgaccaggt tggcaatcgc cttccccttg ccgtcgggac cgacgtcggg gatctcgtac
     3961 accttcagcc agtagacgcg ccccttgtcg ctgaagatca ggatgtacga gtgggtcgag
     4021 gcgatgaaga ggtgcgcgac gaaatcctcc tcgcgcacgc tcatcccccg ccgcccgagg
     4081 ccgccccgcc gctgcgtccg gtactcggtc accgcggtcc gcttgatgta cccggtgttg
     4141 ctcaccgtga tcgcgacgtc ctcgtccacg atcaggtcct cgacccggaa ctccccctcc
     4201 tcctcgacga tctcggtgcg ccggccgtcg ccgtacttgt cgcgcacctc gcgcagctca
     4261 tggacgacga tctccatcag cagcgcctcg ctgccgagga tggcccggta gcgctcgatc
     4321 tgccgcagca cctcggccag ctcgtcgagg atcttctgcc gctcgaggcc cgtcagccgc
     4381 tgcagctgca tgtcgaggat ggcctgcgac tgcacctggc tcaggccgaa ccgcgtcatc
     4441 aggccgtcgc gcgcctcgag cggcgacttc gccgcccgga tgagcgcgat cacctcgtcg
     4501 aggtggtcga gcgcgatctt cagcccctcg aggatgtggg cccgcgcctc ggccttccgc
     4561 agcaggtact cggtccgccg ccgcaccacc tcgcgccgga actcgacgaa ctgctcgagc
     4621 acctccacca gcggcagcac caccggccgg ccgccgacaa tcgccagcat gatgatgccg
     4681 aaggtgctct gcagcggggt gtgcttgtac aggttgttga ggacgacgtc ggccacctcg
     4741 ccgcgcttca gctcgatcac gatgcgcagc ccctggcggt cggactcgtc gcgcaggtcg
     4801 gagatcccct cgatggtctt gtcgcgcgcc agctcggcga tcttctcgac gagcttcgcc
     4861 ttgttcacct ggtacgggat ctcggtgatg acgatcgacg agcggtcgcc cttcgcgctc
     4921 tcctcgacgg tcgcccgcgc ccgcaccgtg atggcgccgc gccccgtccg gtaggcctgc
     4981 acgatgcccg agcgcccgac gatcagcccc gcggtcggga agtcggggcc cggcacgatc
     5041 ttcaggagcg ccttcagctt cgcgtcggcc gacatcggcg gctcgccgcc cgcctgctcg
     5101 cgctgccgct gctcgacgag ccagatggcc ccgtccacca cctcgcgcag gttgtgcggc
     5161 gggatgttgg tggccatgcc caccgcgatg cccaccgacc cgttcaccag caggttcggg
     5221 aagggcgccg gcaggacggt cggctcctcg gtggtctcgt cgtagttggg gacgaagtcc
     5281 accgtctcct tgtcgaggtc ggccatcatc tcctcggcca gcggcttcag ccgggcctcg
     5341 gtgtaccgca tggccgccgg gggatcgccg tccatcgacc cgaagttccc ctggccgtcc
     5401 accagggggg cgcgcatgtt gaagtcctgc gcgaggcgca cgagcgtgtc gtagatcgcc
     5461 gcgtcgccgt ggggatggaa gttccccatg acctcgccga cgatcttcgc gcacttgcgg
     5521 taggcgcggt tgctcgccag ccccatcgtg cgcatgccgt agagcacgcg ccggtgggcc
     5581 ggcttgagac cgtcgcgcac gtcgggaagc gcccgcccga tgattacgct catcgcgtaa
     5641 tccatgtacg agcgcttcat ctcgtcttcg atgttgaccg gcagcttggt gagtgcgtcc
     5701 atgtcttcag gattcaggag tcaggattca ggaatcgggc ggccgtcgcg cagcgcccgg
     5761 gtggtcagga cacgtgattc gcggcggatt gcacccccga atcccgaatc ctgaatcccg
     5821 attcccgcct cagatgtcca ggttcctgac gtccagcgcg ttgtcctcga tgaacttccg
     5881 gcgcggctcc acctggtcgc ccatcagggt ggtgaacatc aggtcggcct cggtgtggtc
     5941 ttcggcccgc acctgcagca gcgtccggct ctcgggcctc atcgtcgtcg accagagctg
     6001 ctcggggttc atctcgccca ggcccttgta gcggttcacc gcgacgcccc gcttgccggc
     6061 cgtcacgaag aactccacca cctggtcgag cgtggtcagc gtgaccgtct cgggatcctt
     6121 cgacgaccgc cccgccgact cgcgcggctc gggcgccggc tcggcggcca gcttggtggc
     6181 cgggtcgatc gccccgcccc cgatccgcgt gtcgttggcc gacgcggtgg gcagccccgg
     6241 gtcgtgctcg tccacctcga cggcgggcgc cgaagtggcg gccaccacga ccggcagctc
     6301 gagcccctgg atctccttgt agctgctggc cagggtccgg tagtccacgc ccgtcaggaa
     6361 gtcgatgtcc agctggtagt gccgcgggta gccgttcgac cggtcctcga cgtgcagcga
     6421 cgagaggttg tgctcctcgt cgggctgcac ggtgaccgtg cgggtggcgc tgctcagctc
     6481 ctcggcgagc tgctgcagcc cctcggcgtc ggcgaagaac gacttgtcgt gcgcgtcgcg
     6541 gtccatcacc gccttgagca cgtcgaactg gtagccgcgc cgctcgacca cctgcaggta
     6601 gcgccggaag gcgatcaggt cgtggagcag cttctcgagg gcgtcgcccg ccagctcgcg
     6661 cccgtccttg aggcgcagca cgcggctctc gatcgcccgc cgcatcagcc agtgctccag
     6721 ctcgcgctcg tccttgatgt agtgctcggc cttcccgcgc ttggcccgga acagcggcgg
     6781 ctgcgcgatg tagacgtagc cgcgctcgat cagctcgggc agctgccggt agaagaaggt
     6841 cagcagcagc gtgcggatgt gcgacccgtc cacgtccgcg tcggtcatga tgatgatgcg
     6901 gtggtaccgg agcttctcga cgtcgaagtc ctcggggccc acgccgcacc cgagcgccga
     6961 gatcatcacc ttgatctcct cgctcgagag catcttgtcg aagcgcgcct tctcgacgtt
     7021 gaggatcttc ccgcgcagcg ggaggatcgc ctggaacttc cggtcgcgcc cctgcttggc
     7081 cgacccgccg gccgactcgc cctcgacgat gtacagctcg gagagggcgg ggtcgcgctc
     7141 ctggcagtcg gccagcttgc ccgggaggct gctgttctcg agcgccccct tgcgccgcac
     7201 caggtcgcgc gccttgcgcg ccgcctcgcg cgcccgcgcc gcgtccaccg acttcatcac
     7261 gatgcgccgc gcgaccgccg ggttctcctc gaggaacgcc cccagcttgt cgttgacgat
     7321 cgcctcgacg atccccttga cctcggtgtt ccccagcttc gtcttggtct gcccctcgaa
     7381 ctgcggccgc gggatcttca cgctgatgac cgccgtcagc ccctcgcgga tgtcgtcgcc
     7441 gccgatgctc tccttgaggt ccttcgtcag gttgttcttg gcggcgtacg cgttcaccgt
     7501 gcgggtgagc gccgcgcgga accccgagag gtgggtgccc ccctcgtgcg tgttgatgtt
     7561 gttggcgaag gtgtagatcg tctcggtgta gccgtcgttc cactggagcg cgatctcggc
     7621 gtcgatcccg tcgcgctcgc cccgcatgta gatcggcttg tcggtgaccc ccgacttgtt
     7681 cttgttcagg tgcgtcacga actcgcggat gccgccgtcg tactggaagc ggtgcccctt
     7741 gccgtcggtc ctgtcgtcct cgagcgtgat ggccacgccc gggttgagga acgccagctc
     7801 gcgcagccgc tgggcgagcg tgtcgaagct gaactcgatg gtttcgaaga cgccgcggtc
     7861 gggcaggaag cggatcttgg tgccgcgctt cttcgtcttg ccggtgatct ccaggtcggc
     7921 ctggggctgg ccgcgctcgt acacctggtg gtacacgagg ccgttgcgcc agatctcgag
     7981 gtcgagcgtc tcggagagcg cgttgaccac cgacacgccc acgccgtgca ggccgcccga
     8041 caccttgtag ctgttgttgt cgaacttgcc cccggcgtgg agcaccgtca tcacgacctc
     8101 ggccgccgac cgcccgctct cgtgcatgtc gacggggatg ccgcggccgt tgtcgacgac
     8161 cgtcaccgag ttgtcgaggt gaatcgtgac gttgatcgtg tcgcagaagc cggccagcgc
     8221 ctcgtctatc gagttgtcga cgacctcgta cacgaggtgg tgaagcccca gcggccccgt
     8281 cgagccgatg tacatggcgg ggcgcttgcg gaccgcctcg aggccctcga gcaccttgat
     8341 cttgtcggcg ccgtactcgt ccacgccgag atcgtgcagc tgctcctcgt ctggtgcgcc
     8401 cgaaccgttc gacggcgcga cttcaggtgt cccggcgctg ttctctgttg cctgcggcga
     8461 gaccgtttcc tcgaacccgt gaccgcttgc gttggtatct gacatgaatc cttgacagct
     8521 ctttgctgct agaagcgcat cggcatcagc acgtaggtgt actggaagcc ctcctgcccc
     8581 accggcttca tcacggcttg gctgacctcg tccttcagct cgagcgccac ccggtcggtc
     8641 tcgaccacct cgaggaactg ctggacgtac tggccgttga agccgatcga cagcgcctcg
     8701 cccgcgtagt cgtccacgcc gaggatctcc ttcgcctcgc cgtactcggg gctctgggac
     8761 gagacctcga cctttcccgg ctcgatggcc agcttgactg cgcgcgaccg ctcgggcgac
     8821 agctgcccga cgcgcttgag cgccgcggcc agccgctcgc gctcgaactc gatcaccttg
     8881 tcgttgccct tggggacc
//
LOCUS       DAOPQX010000127         8872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8860_length_8872_cov_4.466258, whole genome shotgun sequence.
ACCESSION   DAOPQX010000127 DAOPQX010000000
VERSION     DAOPQX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8860_length_8872_cov_4.466258"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKK95_08425"
     CDS             <1..711
                     /locus_tag="PKK95_08425"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC18278.1"
                     /translation="RWEQVVAMFPYTTLLFPPGSKYSYSNPGVIFLGRTIEALSGDDY
                     EMYVTKNILAPLGMSHTFFDRAPYHLLPRRSHSYYRNDEGLQEGRFDFDTGITVSNGG
                     LNAPLGDMAKYLAFLMGGPGQPEAHEGVLARASLEEMFRPVARASDGEGGTGQEVQIG
                     LSFFLERHQGLDLVAHSGSQGGFLSHFYLHLPTRTGFVVAYNTEVTTKKGANPEVTRQ
                     VDAELRDEILKRVFSRAR"
     gene            complement(775..1266)
                     /locus_tag="PKK95_08430"
     CDS             complement(775..1266)
                     /locus_tag="PKK95_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOC18279.1"
                     /translation="MANGTRELDGRDRQILHLVQRDATLAQSEIARQVGLSTAAVHER
                     LKKLEAAGVIRRWTAVVDSAAVGAEVTAFVEVFVEHPRFEPAFLEKLLGLDEVLECHH
                     VTGDFSLLLKVRVQDIAALQRLLHDHVASQEGVRQTRTVVVLSTAKEETYVSPEAKER
                     TQP"
     gene            1469..2704
                     /gene="hydF"
                     /locus_tag="PKK95_08435"
     CDS             1469..2704
                     /gene="hydF"
                     /locus_tag="PKK95_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOC18280.1"
                     /translation="MQSTPKSLRLHIGIFGRRNVGKSSLLNAITRQQVSIVSAFAGTT
                     TDPVEKPMELLPFGPVQFVDTAGIDDEGALGELRMARTRAVLDRVDLGVLVTEAGGWG
                     AFEESLLAELATRRVKTLVVFNKVDERKPSADDLARVEAAGLPHVAVSATGGDGLLDL
                     REALLRLAPSEFLETRFLIADLVPPGEVAVLVIPIDKEAPKGRIILPQVMAIRDLLDG
                     GSMAVVAQAHELRRALEALTRPPALVVTDSQAFAEVAAEVPPGVPMTSFSILMARFQG
                     DLVEQVRGTLAIEQLREGDRILVAETCTHHPIGDDIGRVKIPMWLQKYVGASLAFEYV
                     QGRDFPSTAALRQYQLIVHCGNCTGNRREMASRILRARTAGVPITNYGLTIAYSLGIF
                     ERALVPFPAALEAYHAAAV"
     gene            2688..3761
                     /gene="hydE"
                     /locus_tag="PKK95_08440"
     CDS             2688..3761
                     /gene="hydE"
                     /locus_tag="PKK95_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOC18281.1"
                     /translation="MPLLCEAGTLDVAGIEAWLREADEDCLARLWEAADRVRTERVGA
                     AVHLRGLVEVSNYCVRSCAYCGIAACAGPLPRYRLSREEILACAREARRFGFGTVVLQ
                     SGEDPGLTRRFIGEVVAEIHRDLGLAITLSLGERDDEDLMAWREAGADRYLLRFETTD
                     PALYAAIHPGLPGKVSDRIAQLVRMREMGYEVGTGVMVGIPGQTWATLARDIDTFRQF
                     DMDMIGIGPYLPDPRTPLAGPLGGQLQAPPGEQVPNDEVTTLKTVALTRLVCPDTNIP
                     STTALSILDPGAGRRNGLTRGANVVMPNLTPPHYRQLYQIYPGKAGVHESVETLTENV
                     FRLIAALGRTVGEGPGGREKRVG"
     gene            complement(3843..5288)
                     /locus_tag="PKK95_08445"
     CDS             complement(3843..5288)
                     /locus_tag="PKK95_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18282.1"
                     /translation="MPDLSPRDYRLGLCAVLLLAALAGLWRLGTPPLWLDEASSWYNV
                     SGSWLHLWERAVQAEDSGGLLYSALLKPWSAFAGTGEAALRVPGVVLWLGTIAVIARA
                     TRLAWGRDAALLAASAALAHPSLLAAARQARSYILLFLLAALALLALAEQMAGRRRRG
                     LWLLAAGSLAAAATHVMGVFIALGAATCQLLLGSRERAAALRAALAAAPAFVFAAVWA
                     ILMRGRVGRNLESFWIPGTLLTNYLAIGLLLVAPLAIACAWLLWRDPSTRSRAAVAGL
                     AAFALPVALGPAILSAFAAEGAHLVQLRYALALAPLGAAAAGGALATLPRSLAATTAA
                     LAIAASIGYGVGKQVYAPVTRAGQDVRGAAAFLASTVHPGDLALIEPPNNWMPLAYYG
                     VWTVPLEGQGGGWRVVTAEAKRGGQSAEGRGENDDRGKCGERREGKETGGRTIWLVRF
                     SRQTAAPPDLLQRATLAGRFGTLTILEVTPH"
     gene            complement(5417..6607)
                     /locus_tag="PKK95_08450"
     CDS             complement(5417..6607)
                     /locus_tag="PKK95_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC18283.1"
                     /translation="MMQTLRVLVVDDELGMRLAVARVLGTFVVHLNEEVGGEIHFEVQ
                     TAESGEQAIELMTSQPAPDLLLLDLKMPGISGLEVLAQMRERNLHPLTVMITAYATLE
                     TAIDATKRGAHDFLPKPFTPDELRVAVRRAVKHLMVQRQAQRLREEKRQVRFQFISVL
                     GHELKAPLAAIEGYLQILRDGTAGQSPDTIEQVVQRALVRAHGMRKLISDLLDLTRIE
                     SGQKRREFGPVDFREIAQNALDGVAQAAEAREIALELHAPERVPAVADRGEIEIIFNN
                     LVSNAVKYNRDGGRVDVTIEPVEGGLVRIAVADTGIGLTREDASRLFQEFVRIRNDQT
                     RAIAGTGLGLSIVRKLAQLYQGDATVESEPGVGSTFTVTLNIAAPPPAPMSEPLSGAV
                     PIAR"
     gene            complement(6604..7023)
                     /locus_tag="PKK95_08455"
     CDS             complement(6604..7023)
                     /locus_tag="PKK95_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC18284.1"
                     /translation="MTTTERTRILLVDDDEDFLFQHRIQLENAGFEVSAASSRAQAEQ
                     VLASFTPDLAILDLMMEHHDDGFVLSHHLKRRFPSLPVIMVTAVTSETGLVFDPASPE
                     ERRWVGADALLAKPIRFEQLKREIERLLKAASAAQRG"
     gene            complement(7020..>8872)
                     /locus_tag="PKK95_08460"
     CDS             complement(7020..>8872)
                     /locus_tag="PKK95_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOC18285.1"
                     /translation="RSAIENVEELLADTVPVAAIIAPSFPAEFIDLPYSSVVGTLRAL
                     GFASVHEVAFGAELVALEYNKLVAADTDKLWVATTCPAVVGYIEKYYPDLTDSLAPIV
                     SPMIATARLLRRRHGDSIRIVFVGPCIAKKREAALPELDGEVDEALTFAELRAMVAAR
                     RLDPRTVPPSEFDPPHSRGGGLFPISGGILQVARIQEDLVTGHVMVAEGPEEFAQAIR
                     EAESGLMDTRLLEVLFCKGCTMGPGMSSDLPHFGRRARVSQYVRQRIAQSDKAAWEQR
                     IADHATIDLSRKYRRDDTRMPVPSPEELTRILAEMGKTDPHDELNCGACGYPSCRETA
                     AAIYNGLAEPEMCLPHSVEHLRETVRELAESNEELADAQVALVRSEKLASMGQLAAGI
                     AHEVNNPLGVVIMYSHFLMEQLAGKAEYEGDLAMVVEQANRCKKIVAGLLDFARQSKV
                     EHEQVDVGQLVQRGLRGVAHPIGVEIQVEHAGGDPIIEVDPDQLTQVLVNIFSNAFAA
                     MPDGGRLTIRTDGDDERVSIAVTDTGTGIVPEHLPKLFEPFFTTKKSGKGTGLGLAVT
                     YGIVKMHRGDIKVTSNADPAKGPTGTTFTVVLPRRRQEPASSTEGSTSAA"
BASE COUNT         1315 a         3045 c         3168 g         1344 t
ORIGIN      
        1 cggtgggagc aggtggtggc gatgttcccg tacaccaccc tgctgttccc gccgggatcg
       61 aagtacagct actccaaccc cggcgtgatc ttcctcgggc gcaccatcga ggcgctctcg
      121 ggcgacgact acgagatgta cgtcaccaag aacatcctgg cgccgctcgg gatgtcgcac
      181 accttcttcg accgcgcccc gtaccacctg ctgcccaggc gatcccacag ctactaccgc
      241 aatgacgagg gtctccagga gggacggttc gacttcgaca ccgggatcac ggtctcgaac
      301 ggcggcctga acgctcccct cggcgacatg gcgaagtacc tggccttcct gatgggcggc
      361 ccggggcagc cggaggcgca cgagggcgtg ctggcccgcg cgtcgctcga ggagatgttc
      421 cggccggtgg cccgtgcgtc ggacggcgag ggagggacgg gccaggaggt ccagatcggc
      481 ctttcgttct tcctcgagcg ccaccagggc ctcgacctgg tggcccacag cggcagccag
      541 ggcggcttcc tctcccactt ctacctgcac ctgccgaccc gcacggggtt cgtcgtggcc
      601 tacaacacgg aagtgacgac gaagaagggc gcaaacccgg aggtgacgcg gcaggtggac
      661 gcggagctga gggacgaaat cctgaagaga gtcttcagcc gggcacggtg aaccgttccc
      721 gcctcgatcc tctcctggaa cggatgcggt ttgtcgtcct tcgacgggtg gccgtcaggg
      781 ctgagtccgc tccttggcct cgggcgagac gtaggtttct tccttcgcgg tcgagaggac
      841 cactaccgtc cgcgtctgcc gcaccccttc ctgcgacgcg acgtggtcgt gcagcaggcg
      901 ctggagagcg gcgatgtcct gcaccctcac cttcagcagg agcgagaagt cgccggtcac
      961 gtggtggcac tcgagcacct cgtcgaggcc gagcagcttc tcgaggaacg cgggctcgaa
     1021 ccgcgggtgc tccacgaaca cctcgacgaa cgcggtcacc tcggctccga cggccgcgga
     1081 gtcgacgacc gcagtccagc ggcggatgac gccggcggcc tcgagcttct tgagccgctc
     1141 gtgcacggcg gccgtcgaca ggccgacctg ccgcgcgatc tccgactggg cgagcgtcgc
     1201 gtcgcgctgg accaggtgga ggatctgccg atcgcgcccg tccagctcgc gtgtgccgtt
     1261 ggccatagcg tctgaaatcg tagcttaccc gccacggatg gggcaagaaa tggttcactc
     1321 tataccccct tgaaccgaac aattgtcgga ctcaggcaag ccgtcacgcg gagggatggc
     1381 cgtgagcgcg attgggattg cgattcgggt gggatgggat ggggattggg gatgggatgg
     1441 cgattgggat tgatatgatc gatcggctat gcagtccacc ccaaagtcgc tccgccttca
     1501 catcgggatc ttcggccgcc ggaacgtcgg aaagtcgtcg ctcctgaacg cgatcacccg
     1561 gcagcaggtt tccatcgtgt cggcgtttgc cggcacgacc accgacccgg tggagaagcc
     1621 catggaactg ctgccgttcg gcccggtcca gttcgtcgac acggccggga tcgacgacga
     1681 gggggcgctc ggcgagcttc gcatggcgcg cacccgggcc gtgctcgacc gcgtggacct
     1741 gggggtcctg gtgaccgagg ccggagggtg gggggccttc gaagagtcgc tgctggccga
     1801 gctggccacg cgccgcgtga agacgctggt ggtcttcaac aaggtggacg agcggaagcc
     1861 gtcggccgac gatctcgcca gggtggaggc ggccgggctg cctcacgtgg cggtgtcggc
     1921 caccggtggc gacggtctgc tcgacctgcg cgaggcgctc ctgcggctcg cgccgtccga
     1981 gttcctcgaa acccggttcc tgattgccga cctcgtcccc cccggcgagg tggccgtcct
     2041 cgtgatcccg atcgacaagg aggcgccgaa ggggcggatc atccttcccc aggtcatggc
     2101 catccgcgac ctgctcgatg gcggctcgat ggcggtggtg gcgcaggcgc acgagctgcg
     2161 ccgcgccctc gaggcgttga cgcggccgcc cgccctggtg gtgaccgact cgcaggcgtt
     2221 tgccgaggtg gccgccgaag tcccgcccgg ggtgccgatg acctccttct cgatcctcat
     2281 ggcgcgattc cagggcgacc tggtcgagca ggtgcggggg acgctcgcga tcgagcagct
     2341 gcgcgagggc gaccgcatcc tggtggccga gacctgcacg caccacccga ttggcgacga
     2401 catcggccgc gtcaagatcc cgatgtggct gcagaagtac gtcggggcgt cactggcgtt
     2461 cgaatacgtg cagggccgcg acttcccgtc cacagccgcg ctgcgccagt accagctgat
     2521 cgtccactgc ggcaactgca ccggcaaccg gcgggagatg gcgtcgcgga tcctgcgcgc
     2581 gagaacggcc ggcgtgccaa tcaccaacta cggcttgacg attgcctact cgctggggat
     2641 cttcgagcgg gccctggtcc cgttcccggc tgccctcgag gcctaccatg ccgctgctgt
     2701 gtgaagcggg cacgctcgac gttgccggca tcgaggcctg gctgagggag gccgacgagg
     2761 attgcctcgc gcgcctctgg gaggcggccg accgggttcg caccgagcgg gtaggagcgg
     2821 cggtccacct tcgcgggctc gtggaggtga gcaactactg cgtgcgcagc tgcgcgtact
     2881 gcggcatcgc ggcctgcgcc ggcccgctcc cgcggtaccg gctgtcgcgc gaggagattc
     2941 tcgcctgcgc ccgcgaagcg aggcgtttcg gcttcggcac cgtcgtcctc cagtcgggcg
     3001 aggaccccgg cctgacgcgc cgcttcatcg gcgaggtggt cgccgaaatc catcgcgacc
     3061 tggggctcgc gatcacgctg agcctgggcg agcgagacga cgaggacctc atggcgtggc
     3121 gggaggcggg tgcggaccgc tacctgctgc ggttcgagac caccgacccc gcactctacg
     3181 ctgccatcca ccccggcctg ccgggcaagg tgtcggaccg catcgcgcag ctggttcgga
     3241 tgcgcgagat gggatacgag gttggcaccg gcgtgatggt cggcatcccg gggcagacct
     3301 gggcgacgct ggcgcgcgac atcgacacgt tccggcagtt cgacatggac atgatcggca
     3361 tcgggcccta cctgccggat ccgcgcacgc cgctggccgg gccgctcggc gggcagctgc
     3421 aggcgccgcc gggcgagcag gtcccgaacg acgaggtgac gacgctcaag acggtcgcgc
     3481 tgacacgcct ggtctgtccc gacaccaaca tcccgagcac gacggcgctg tcgatcctcg
     3541 acccgggcgc gggacggcgc aacggcctca cgcgcggtgc caacgtcgtg atgccgaacc
     3601 tgacgccgcc gcactaccgg cagctgtacc agatctaccc gggaaaggcc ggcgtccacg
     3661 agtcggtcga gacgctgacc gagaacgtct tccggctgat tgccgcactc gggcggacgg
     3721 ttggggaggg gcccgggggg cgcgaaaaga gggtcggata gaagcgagac gacgatttca
     3781 ccgcagaggc gcagagcacg cagagacgaa ccgggtaatc caggcgtctc cgcgttgaaa
     3841 gctcagtgcg gcgtgacctc gaggatggtc agcgtgccga agcggccggc gagggttgcc
     3901 cgttggagca aatctggcgg cgccgccgtc tgccgcgaga acctcacgag ccagatcgtc
     3961 cgtcccccgg tctccttccc ttccctccgc tctccgcact tgccgcggtc atcgttctcc
     4021 ccgcggccct cggcgctctg gcctccgcgc ttcgcctccg ccgtaaccac ccgccaccct
     4081 ccgccctgtc cttcgagcgg cacggtccac acgccgtagt acgccagcgg catccagttg
     4141 ttgggtggct cgatcagcgc gaggtcgccg gggtgcacgg tggaggcaag gaacgcggcc
     4201 gcgccgcgaa cgtcctgccc ggcgcgcgtc accggcgcgt agacctgctt gccaaccccg
     4261 taaccgatgg aggcggcaat cgccagcgcg gccgtcgtgg cggccaggga acggggcagt
     4321 gtggccagtg cgccgccggc tgccgcggcg ccgagcggag cgagtgccag cgcgtagcgc
     4381 aactgcacga ggtgcgcgcc ctccgccgcg aaggccgaca ggatcgccgg gccgagggcg
     4441 acgggcaggg cgaaggcggc caggccggcg acggcggcgc gcgagcgagt cgaggggtca
     4501 cgccacagga gccacgcgca ggcgatcgcg agcggcgcga cgagcaggag accgatcgcc
     4561 aggtagttcg tgaggagcgt tccggggatc cagaacgact cgaggttgcg gcccacgcgg
     4621 ccgcgcatga ggatggccca gacggcggcg aacacgaagg ccggcgcggc tgcgagtgcc
     4681 gcgcgcaggg ctgccgcccg ttcgcggctt ccaaggagga gttggcaggt cgcggcgcca
     4741 agggcgatga agacccccat cacgtgggtg gcggccgcgg cgagcgagcc ggcggcaagc
     4801 agccagagtc cgcgccggcg gcgtcccgcc atctgttcgg ccagggcgag tagcgccagc
     4861 gcggccagga ggaacagcag gatgtaggag cgggcttgcc gggcggcggc cagcaggctg
     4921 ggatgggcga gcgccgccga cgcggcaagc agcgccgcgt cgcggcccca cgccagacgg
     4981 gtggctcgcg cgatgacggc gatggtgccg agccacagca cgacgcctgg cacgcggagc
     5041 gccgcctcgc cggttccggc gaacgcgctc cagggtttca gcagcgccga gtagagcagc
     5101 ccgcccgagt cttcggcctg cacggcgcgc tcccacaggt ggagccagct tcccgagacg
     5161 ttgtaccagg acgaggcctc gtccagccag aggggcgggg tgcccaggcg ccacaggccg
     5221 gccagtgccg ccagcagcag cacggcacaa aggcccaggc ggtagtcgcg gggtgagagg
     5281 tcgggcacgg gcgaaaggcg aagcgtagga tatcagagac agaaacgggc cggccccggc
     5341 gcggcggtcc cccccaggtc cgaccacgcc gaaaccggcc ctcacgaaac acgatccgca
     5401 gggctaaagc cctgcgctac ctggcgatcg gcaccgcgcc cgagagaggc tcgctcatcg
     5461 gggcgggcgg cggtgcggcg atgttcagcg tcaccgtgaa cgtgctgccg acgccaggct
     5521 cgctctccac cgtggcgtcg ccctggtaca gctgcgccag cttccggacg atcgacagcc
     5581 cgagccccgt gcccgcgatc gcccgcgtct ggtcgttccg gatgcggacg aactcctgga
     5641 acagccgcga cgcgtcctcc cgcgtcagcc cgatcccggt gtcggcgacg gcaatgcgaa
     5701 cgaggcctcc ctcgacgggt tcgatggtca cgtccacccg tccgccgtcg cggttgtact
     5761 tcaccgcgtt gctgacgagg ttgttgaaga tgatctcgat ctccccccgg tcggccaccg
     5821 ccggcacgcg ttcgggcgcg tgcagctcga gcgcgatctc cctggcctcg gcggcctggg
     5881 cgacgccgtc gagcgcgttc tgcgcgatct cgcggaagtc gaccggcccg aactcgcggc
     5941 gcttctggcc cgactcgatc cgggtcaggt cgagcaggtc gctgatcagc ttgcgcatcc
     6001 cgtgcgcccg caccagtgcg cgctggacga cctgttcgat ggtgtcgggg ctctggccgg
     6061 ccgtcccgtc gcggaggatc tggaggtagc cctcgatcgc ggcgagcggc gccttcagct
     6121 cgtggccgag caccgagatg aactggaagc ggacctgccg cttctcctcg cgcaggcgct
     6181 gcgcctgccg ctgcaccatc aggtgcttga cggcgcggcg cacggcgacg cgcagctcgt
     6241 cgggggtgaa cggcttcggg aggaagtcgt gcgccccgcg cttggtggcg tcgatcgcgg
     6301 tctcgagggt ggcgtaggcc gtgatcatca ccgtcagcgg gtgcaggttc cgctcgcgca
     6361 tctgcgccag cacttcgagc cccgagatgc cgggcatctt gaggtcgagc agcagcaggt
     6421 cgggcgccgg ctgcgaggtc atcagctcga tggcctgctc gcccgactcg gccgtctgca
     6481 cctcgaagtg gatctcgccg ccgacctcct cgttcaggtg cacgacgaag gtgccgagca
     6541 cgcgagcgac ggccaggcgc atccccagct cgtcgtcgac gaccaggacg cggagcgtct
     6601 gcatcatccc ctctgcgcgg cggaggcggc cttgagcagc cgctcgatct cgcgcttcaa
     6661 ctgctcgaag cggattggct tggccagcag ggcgtcggcc ccgacccagc ggcgttcctc
     6721 gggcgaggcc gggtcgaaga cgagccccgt ctcgctggtg accgccgtca ccatgatgac
     6781 cgggaggctc gggaaccggc gcttcaggtg gtgcgagagc acgaacccat cgtcgtggtg
     6841 ctccatcatc aggtcgagga tcgccaggtc gggggtgaac gaggcgagca cctgctcggc
     6901 ctgggcccgg ctcgaggccg cagacacctc gaacccggcg ttctcgagct ggatccggtg
     6961 ctggaacagg aaatcctcgt cgtcgtccac gaggaggatt cgggtgcgtt ctgtggtcgt
     7021 catgccgctg acgtactccc ttcggtgctg ctggccggct cctggcgccg cctcgggagc
     7081 acgacggtga aggtcgtgcc cgtgggcccc ttggccgggt ccgcgttcga tgtgaccttg
     7141 atgtcgcccc ggtgcatctt cacgatgccg taggtgacgg cgaggccgag ccccgtgccc
     7201 ttgccgctct tcttggtcgt gaagaacggc tcgaacagct tggggaggtg ctcgggcacg
     7261 atgccggtcc cggtgtcggt cacggcgatc gacacgcgct cgtcgtcgcc gtcggtccgg
     7321 atcgtgagac ggcccccgtc gggcatcgcg gcgaaggcgt tgctgaagat gttcacgagc
     7381 acctgggtca gctggtccgg gtcgacctcg atgatcgggt cgccgccggc gtgctcgacc
     7441 tggatctcga cgccgatcgg gtgcgccacg ccgcggaggc cccgctgcac gagctggccg
     7501 acgtccacct gctcgtgctc caccttgctc tggcgggcga agtcgagcag ccccgccacg
     7561 atcttcttgc agcggttcgc ctgctcgacc accatcgcga ggtcgccctc gtactccgcc
     7621 ttcccggcga gctgctccat caggaagtgc gagtacatga tgaccacgcc gagggggttg
     7681 ttgacctcgt gggcgatccc cgccgccagc tgccccatgc tggccagctt ctccgagcgg
     7741 acgagcgcca cctgggcgtc ggccagttcc tcgttcgact cggccagctc gcgcaccgtc
     7801 tcgcgaaggt gctcgacgct gtgcggcagg cacatctccg gctcggccag gccgttgtag
     7861 atggcggccg ccgtctcgcg gcagctcggg tacccgcacg cgccgcagtt cagctcgtcg
     7921 tgcgggtcgg tcttgcccat ctcggccagg atgcgcgtca gctcctcggg cgaggggacc
     7981 ggcatccgcg tgtcgtcccg gcggtatttc cgggagaggt cgatcgtcgc atggtccgcg
     8041 atgcgctgct cccaggccgc cttgtcgctc tgcgcaatcc gctggcggac gtactggctg
     8101 acgcgggcgc ggcggccgaa gtgcgggagg tccgagctca tgcccgggcc catcgtgcac
     8161 cccttgcaga agaggacctc gagcagccgc gtgtccatca ggccgctctc ggcctccctg
     8221 atcgcctggg cgaactcctc ggggccctcg gccaccatca cgtgcccggt caccaggtct
     8281 tcctggatgc gtgcgacctg caggatgccg ccgctgatcg ggaacaggcc gccgccgcgg
     8341 ctgtgcggcg ggtcgaactc cgaggggggc acggtgcgcg ggtcgaggcg ccgggccgcg
     8401 accatcgcac gcagctcggc gaaggtcagg gcctcgtcca cctcgccgtc cagctcgggg
     8461 agtgccgcct cgcgcttctt ggcgatgcac ggaccgacga agacgatccg gatgctgtcg
     8521 ccgtggcggc ggcggagcag ccgcgcggtg gcgatcatcg gcgacacgat gggggcgagc
     8581 gagtcggtca ggtcggggta gtacttctcg atgtacccga ccaccgccgg gcaggtggtg
     8641 gccacccaga gcttgtcggt gtcggctgcc accagcttgt tgtactcgag agcgaccagc
     8701 tcggcgccga acgccacctc gtggaccgac gcgaagccga gcgcccgcag cgtgccgacc
     8761 accgacgagt aggggaggtc gatgaactcc gccgggaagc tcggggcgat gatggcggcc
     8821 accggcaccg tgtcggccag cagctcctcc acgttctcga tcgccgatcg ca
//
LOCUS       DAOPQX010000128         8836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8897_length_8836_cov_4.934290, whole genome shotgun sequence.
ACCESSION   DAOPQX010000128 DAOPQX010000000
VERSION     DAOPQX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8897_length_8836_cov_4.934290"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKK95_08465"
     CDS             complement(<1..711)
                     /locus_tag="PKK95_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC18286.1"
                     /translation="MATIAAVAVGLTALASCEQTPGDPTNSRTASQAAATGGPTVVSL
                     VPAVTEMLFAVGAGPQVVGVGSFDNWPPEVRRLPRLGALLDPDVERILRLRPGVLVLH
                     VSQEDLRKQMERAGIQVFAHATGGLDDVPRNMREIGALVGRAAEAEEAAAAFEQGLAE
                     IRAHAAKRTQPRTLIVFGHEPGTLRAIDASGGIGFLHDVVTLAGGENVFAPVKRESVR
                     VSTEAIIASIFSTSDRIAA"
     gene            complement(801..1802)
                     /locus_tag="PKK95_08470"
     CDS             complement(801..1802)
                     /locus_tag="PKK95_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOC18287.1"
                     /translation="MPMRRRLAFTVLTFGALAGAACLLAPLIGSTPLSLRRVFDGSVP
                     FADNPDAQIFFVARLPRTLAAALVGSSLAAAGVVFQALLRNPLATPFTLGVSAGAALG
                     AMLAIIFPVGLAFAGVSSVPIASFAGALGAVAIVYALATVRRQGLSTTVLLLAGITLN
                     AFFSSVILFIQYLADFSQTFRTVRWLMGDLDVGSYTPILAALPFMLVAFAAFAVLPRA
                     LNLLTLGADAAAARGVDVLRTQRLSFFSASLATGAAVSLGGPVGFIGIIVPHLVRLLV
                     GSDHRIVLPAATLFGAAFLIACDTASRTVMAPMELPVGILTALIGGPFFLWLLLRRR"
     gene            complement(1802..2605)
                     /locus_tag="PKK95_08475"
     CDS             complement(1802..2605)
                     /locus_tag="PKK95_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18288.1"
                     /translation="MLLADDLTYEHPPGPGQQTPGGVRHVSMEIPRGGIVGILGPNGS
                     GKTTLLRLMAGLIKPASGRVLLNGRPLASRSRREVAREIAVVPQETRLAFDFTVLEVV
                     LMGRFPHLGAFQLEGDEDARLARQALDETGTAHLAGRMFPTLSGGEKQRVIIAAALAQ
                     AAPVMLLDEPTASLDPGSQIEIGALLRRLNGRRQVTLAVATHDLNFAASLCTGLVLLR
                     QGRVAAAGPTSEVLTREHIRGVYGVDADVSFNERAGHLTVVPLAQVGAS"
     gene            complement(2589..4916)
                     /locus_tag="PKK95_08480"
     CDS             complement(2589..4916)
                     /locus_tag="PKK95_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC18289.1"
                     /translation="MAALRFPFLTLVFVVCLAGLWPAPVLTQSPAEQDRGDPRTQNGE
                     VGAVRGVALDPAGDVVPHAGIVLATPVGLAASTEADESGRFTFTGVRPGRYELRAASP
                     GLAAGPVAVEVGADRVTELRLTLRLTAISESVVVSAAQVELPLSRAATPVTVISREEM
                     EARQAESIADALRGVQGLSVARNGGRGSVTSVFPRGGDSDFTLVLVDGIKVNAFGGGF
                     DFSTLAAADVERVEVVRGPESALFGSDAIGGTVQVVTRRGGAPRASVAVEHGSLATSR
                     ASLSASGSRGAWSWGGSAGRTSSDGYTGIAPATGERVGNDDFLLLEAGGSLGWRATPS
                     SDLRVTARWTSHERGYPGPFGSNPIGAYTTVDLLSRGETDTRLAGARWRQVLGGDGGR
                     ATLSADASHLEMRNDFTSSYGLSASRTRRSQAQLAVNVDLSEAAAITVGGTWQGERAT
                     SSFIAGDGEALLPIERRNIAAFAEGRLQPHASLSLTAGIRAEGVRRAAMPAYSDPLSP
                     RPAFDARTDTAVNPRVAIAWSLPGRTGSPLSWSRVRAAAGTGMRAPDALEIAFTDNPA
                     LRPERARSVELGIEQAWAGDRLVAGLTAFYNRYDDLIVSVGPVLRNASRYRTDNISNA
                     RARGLEASFAARTGWGLEVRAAYTRLSTAILAVDRLGFAPPPFEPGDRLLRRPRDEGS
                     LDLLFARGPLTSFARIGARGRVLDVEPSWGSYGGLFWNEGYAVVDAGASFRLHRRLTV
                     LVRGGNLLDRRYEESFGFPALGRTATIGVRLAAGR"
     regulatory      complement(5007..5207)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5330..5929
                     /locus_tag="PKK95_08485"
     CDS             5330..5929
                     /locus_tag="PKK95_08485"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOC18290.1"
                     /translation="MAHELPPLPYAYDALEPHVDEQTMRIHHDKHHGTYVTNLNAALE
                     PHADLQKLTAEELLQQIAKVPEAIRTAVRNNGGGHVNHTMFWEIMKPGGGAFSGRIAD
                     AVKGTFGSFDAFRDQFNKAALGRFGSGWAWLVQGADGKLSIESTANQDSPLIEGKWPV
                     FGLDVWEHAYYLKYQNRRADYVNAWWNVVNWEVISGRLK"
     gene            complement(5989..6309)
                     /locus_tag="PKK95_08490"
                     /pseudo
     CDS             complement(5989..6309)
                     /locus_tag="PKK95_08490"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(6328..6465)
                     /locus_tag="PKK95_08495"
     CDS             complement(6328..6465)
                     /locus_tag="PKK95_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18291.1"
                     /translation="MRESLLELGAKAPEFTLPTHEGMPRSLGEFLAQGPLLLAFHRGT
                     W"
     gene            complement(6536..7744)
                     /locus_tag="PKK95_08500"
     CDS             complement(6536..7744)
                     /locus_tag="PKK95_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOC18292.1"
                     /translation="MRPIRETIPLDEARALMAGAARPIERVETVPLEQAHQRVLARAI
                     VAAQDVPPFARAAMDGFAVVAADTFGAGQFDPKTARVIETVHTGEMPTRTVSRGECVQ
                     IATGAPMPGGADAVVMVEETERAEDGTVHIFSPVYPGQNVGRQGADIRAGQEVLGAGD
                     FLTSSRIGAIAALGVAEVAVFGRPRVALLSTGNEIVEPGRPLPPGHIYDINRFTLSAI
                     VSEHGGIPVPRATSADRLDDLSRALDECLDTDLVVFSGGSSVGERDLVLDLIASRGEV
                     TFHGIAVKPGKPTLFGVIGGTPVLGMPGYPTSCLSNGYMLLVPLLRRLARLPEAATRT
                     LHLPLGQRVVSTTGRHQFYTVRIVDGVAMPAFKASGDITSMSLADGYIEIPAQTDIVE
                     KGEVVEVKLF"
     gene            complement(7753..8256)
                     /locus_tag="PKK95_08505"
     CDS             complement(7753..8256)
                     /locus_tag="PKK95_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOC18293.1"
                     /translation="MGHLEHKAQSPAVANCYILTISDTRTEATDTSGRAIFDLLFAGG
                     HQVVGRKLLRDEPDQVRAAILAELENPAVQVIITTGGTGITSRDTTFEAIDSLLEKRL
                     DGFGELFRMLSYDEIGSAAMLSRACAGLARNKVIISLPGSEHAVRLAMTRLILPELGH
                     LVREASR"
     gene            complement(8389..8772)
                     /locus_tag="PKK95_08510"
     CDS             complement(8389..8772)
                     /locus_tag="PKK95_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOC18294.1"
                     /translation="MSRPVLSPSTFSLTVERSKFHALLTPLSSAAEMETLLKARRRDY
                     RKANHHCWALRLADPSGTVVEQSKDDGEVGHPGRVLLELLRKHDLEGALLVSRIFGGV
                     KLGVGGVSRAFRDAGQGAINAHLGQ"
BASE COUNT         1339 a         3128 c         3177 g         1192 t
ORIGIN      
        1 ggccgcgatc ctgtccgatg tcgagaaaat ggaggcgatg atcgcctcgg tgctcacccg
       61 cacggattcg cgcttcaccg gcgcgaacac gttctctccc ccggccaggg tcacgacgtc
      121 gtgcaggaac ccgatcccgc cgctcgcgtc gattgcccgc agcgtgcccg gttcgtgccc
      181 gaagacaatc agggtacgcg gttgcgtgcg cttggccgcg tgcgcgcgga tctcggcgag
      241 accctgctcg aacgccgccg ccgcttcctc agcttccgcc gctcgcccga ccagcgcgcc
      301 aatctctcgc atgttcctcg gcacatcgtc caggccgccg gtggcgtggg cgaacacctg
      361 gatgcccgcc cgctccatct gcttccgcag gtcctcctgc gatacgtgca gcacgaggac
      421 gcccggcctg aggcggagga tgcgctcgac gtcgggatcg agcagcgcgc cgagccttgg
      481 cagccgtcga acctcgggcg gccagttgtc gaagctgccg acgccgacga cctgcggccc
      541 cgcgccgacg gcgaagagca tctcggtcac cgccggcacg agcgacacga ccgtcggtcc
      601 acctgtcgca gccgcctgcg atgccgtcct ggagttggtg ggatctcccg gtgtctgctc
      661 gcagctcgcg agcgccgtga gccccactgc gacggccgct atcgtcgcca cggcccggaa
      721 cacttgttcc cgaaccgtcc gtcgtcttcg cgtctccgcg tctctgcgtt gaacgtgtgc
      781 catgggttcg gcgcccgctc ctacctccgc ctcagcagca gccacaggaa gaacggtccg
      841 ccgatgagcg ccgtgaggat gccgaccggc agttccatgg gcgccatcac cgtgcgcgac
      901 gcggtgtcgc acgcgatcag gaacgctgcc ccgaagagcg tggccgccgg cagaacgatc
      961 cggtggtccg accccaccag caggcgcacc aggtgcggca cgatgatccc gatgaacccc
     1021 accgggccgc cgagcgagac tgcggcgccg gtggcgagcg aggcgctgaa gaaggagagc
     1081 cgctgcgtcc gcaggacgtc cacccctcgc gccgcggcgg cgtcggcccc gagcgtgagc
     1141 aggttcagcg cacgcggcaa caccgcgaac gccgcgaagg cgaccagcat gaatggcagc
     1201 gcggccagga tcggcgtgta gctccccacg tcgaggtcgc ccatcagcca ccgcaccgtc
     1261 cggaacgtct gcgagaagtc ggccaggtac tggatgaaga ggatcacgga cgagaagaag
     1321 gcgttcaggg tgatcccggc gagcagcagc accgtggtgg acaggccctg ccgccggacc
     1381 gtggccagcg cgtagacgat ggcgactgcc ccgagggcgc cggcaaagct ggcgatcggc
     1441 accgacgaga cgccggcgaa ggccaggccc accgggaaga tgatcgcgag catggcgccg
     1501 agcgcggcgc cggctgagac ccccagggtg aacggggtcg cgagcgggtt ccgcagcagc
     1561 gcctggaaca ccacccccgc ggcggcgagc gaggagccga ccagggctgc cgcgagcgtc
     1621 cggggcaggc gcgcgacgaa gaagatctgc gcgtcggggt tgtcggcgaa ggggacgctc
     1681 ccgtcgaaga cgcggcgcag cgagaggggc gtggagccga tcagcggggc gagcaggcac
     1741 gcggcccccg ccagcgcccc gaaggtcagc acggtgaagg ccaggcgccg ccgcatcggc
     1801 atcagctcgc cccgacctgc gccagcggga cgaccgtcag gtgcccggcg cgctcgttga
     1861 aggagacgtc ggcgtccacg ccgtacacgc cgcggatgtg ctcgcgggtc aggacctcgg
     1921 aggtcggccc ggcggcggcc acgcggccct gccgcagcag caccaggccg gtgcacaggc
     1981 tggcggcgaa gttcaggtcg tgcgtcgcga cggcgagcgt cacctgccgg cgcccgttca
     2041 gccgccggag cagcgccccg atctcgatct gcgagccggg atcgagcgac gcggtcggct
     2101 cgtcgagcag catcaccggc gccgcctgcg cgagcgcggc ggcgatgatg acgcgctgct
     2161 tctcgccgcc gctcaaggtg gggaacatgc ggccggcgag gtgggccgtc cccgtttcgt
     2221 cgagcgcctg gcgggcgagc cgggcgtcct cgtcgccctc caactggaag gcgccgaggt
     2281 ggggaaagcg gcccatcagc acaacctcga gcacggtgaa gtcgaaggcc agccgcgtct
     2341 cctgaggcac caccgcaatc tcgcgggcca cctcgcggcg cgaccgcgag gccagcggcc
     2401 ggccgttcag cagcacgcgc cccgacgccg gcttgatcag ccccgccatc agccgcagca
     2461 gcgtcgtctt gcccgagccg ttgggcccga ggatgccgac gatgccgccc cgggggattt
     2521 ccatcgagac gtggcgcacc ccgcccggcg tctgctggcc cgggccgggc gggtgctcgt
     2581 aggtgaggtc atcggccagc agcaaggcgt accccgatcg tcgcggtgcg gccgagcgcg
     2641 gggaagccga agctttcctc gtaccgccgg tcgagcaggt tgccgcccct cacgagcacg
     2701 gtgaggcggc ggtgcaggcg gaaggaggcc cccgcgtcca cgaccgcgta gccttcgttc
     2761 cagaacaggc cgccgtagct gccccacgag ggctcgacgt ccagaacgcg tccgcgggcg
     2821 ccgatgcgcg cgaacgaggt cagcggcccg cgcgcgaaga gcaggtcgag cgatccctcg
     2881 tcgcggggac ggcgcagcag ccggtcgccc ggctcgaagg ggggcggcgc gaagcccagg
     2941 cgatcgaccg ccaggattgc ggtggacaac cgcgtgtagg ctgcgcgcac ctcgaggccc
     3001 cagcccgtgc gcgcggcaaa ggacgcctcg agcccccgtg cgcgcgcgtt cgagatgttg
     3061 tcggtgcggt accggctcgc gttgcgcagc accggcccga ccgagacgat caggtcgtcg
     3121 tatcggttgt agaaggcggt gagcccggcg acgaggcggt cgcccgccca cgcctgctcg
     3181 atgcccagct cgacgctccg cgcccgctcg ggtctcaacg cggggttgtc ggtgaacgcg
     3241 atctcgagcg cgtccggggc gcgcatcccg gtgcccgcgg cggcccgcac tcgcgaccag
     3301 gagagaggcg atccggtgcg gcccggcagc gaccaggcga tggcgacccg ggggttgacc
     3361 gccgtgtcgg tgcgggcgtc gaaggccggc cgcggcgaga gcgggtcgga gtaggccggc
     3421 atggcggcgc ggcggacgcc ctcggccctg atccccgccg tcagggagag gctggcgtgc
     3481 ggctgcaggc gtccttcggc aaaggcggcg atgttccgcc gctcgatcgg cagcagcgcc
     3541 tcgccgtcgc ccgcgatgaa ggagctggtg gcccgctcgc cctgccaggt ccctcccacg
     3601 gtgatcgcag ccgcctccga caggtcgacg ttgacggcaa gctgcgcctg ggaacgcctc
     3661 gtgcgcgagg ccgacagccc gtacgagctc gtgaagtcgt tgcgcatctc gaggtggctg
     3721 gcatcggccg agagcgtcgc gcgcccgccg tcgcccccga gcacctggcg ccagcgcgcg
     3781 cccgccagcc gggtgtcggt ctcgccccgc gagagcaggt caaccgtggt gtacgcgccg
     3841 atggggttgg aaccgaaggg gccgggatag ccgcgctcgt gcgacgtcca cctcgccgtg
     3901 acccgcaggt cggacgaggg cgtcgctcgc cagccaagcg aaccgccggc ctcgagcagg
     3961 aggaagtcgt cgttgccgac gcgttcgccg gtggccggcg cgatgcccgt gtagccgtcg
     4021 ctcgacgtcc ggcccgccga cccgccccag ctccacgccc cgcgcgagcc cgaggccgag
     4081 agcgacgcgc gcgacgtggc gaggctgccg tgctcgacgg caacgctggc ccgcggcgcg
     4141 ccgccgcggc gcgtcaccac ctgcaccgtc ccgccgatcg cgtcgctgcc gaacagcgcg
     4201 ctctcgggac cgcggaccac ctcgacccgc tcgacgtcgg ccgccgcgag cgtggagaag
     4261 tcgaagccgc cgccgaacgc gttcaccttg atcccgtcca ccagcaccag cgtgaagtcg
     4321 gagtcgccgc cgcgcgggaa gaccgacgtc acgcttcccc ggccgccgtt gcgcgcgacc
     4381 gagagcccct gcaccccgcg cagcgcgtcg gcgatcgact ccgcctggcg cgcctccatc
     4441 tcctcgcgcg agatcaccgt cacgggggtg gctgctcgcg acagcggcag ctcgacctgg
     4501 gcagccgaca cgaccaccga ctcggagatc gcggtcaacc gcagcgtcag ccgcagctcg
     4561 gtcacccggt ccgccccgac ctcgaccgcc accgggccgg ccgcaagccc cggcgaggcg
     4621 gcgcgcagtt cgtaccgccc tggcctgacg ccggtgaaag tgaaccgccc cgactcgtcg
     4681 gcctcggtcg aggcggccag gccgaccggc gtcgccagca cgatgcccgc atgcggcacg
     4741 acgtcgccgg cagggtcgag cgcgacgccc cttacggcgc caacctcccc attctgggta
     4801 cgtgggtcgc cacgatcctg ttcggccggg ctctgggtca gaacaggggc cggccacagg
     4861 ccagcgagac agacgacgaa aacgagcgtg aggaatggga aacgcagtgc agccatgggc
     4921 cggtccttcg gcgtctcacg cgccgatccc cgtgcggtcc cgcctgggag gcgggcgcac
     4981 cgggtcaagg gacgtgccgg gcggccaggt ttcctgactc gcggatcacc gcggcgctgc
     5041 tgccttccca tgcctcgtga ggcaaccctc cagggttgcc ggctcggcac agtggctggg
     5101 tctcttcgtg aggcggccca tcaggcctgc cagtcgatcg accccgtgcg gcgctcgctc
     5161 cccgcctaca gtggcgtgac cgtgtgggct ttccacccac ttcgcttggt cgccggcgaa
     5221 gcgtcggttc gggcagaagt atagcatgtg gcataatgga gactcatctg gtccgcatta
     5281 gtgtgacgag gggcagccct tcggttgccc cgcaatcagg aggacgacct tggcccacga
     5341 gctgcccccg ctcccctatg cctacgacgc cctggagccg cacgtcgacg agcagacgat
     5401 gcggatccac cacgacaagc atcacggcac gtacgtcacg aacctcaacg ccgccctcga
     5461 gccgcacgcc gacctgcaga agctcacggc cgaggagctg ctgcagcaga tcgcgaaggt
     5521 gcccgaggcg atccgcaccg ccgttcgcaa caacggcggc ggccacgtca accacacgat
     5581 gttctgggag atcatgaagc cgggcggcgg cgccttttcg ggacgaatcg ccgacgccgt
     5641 caaggggacc ttcggcagct tcgacgcctt ccgcgaccag ttcaacaagg cggcgctcgg
     5701 gcgcttcggc agcggctggg cctggctcgt gcagggggcc gacggcaagc tgtcgatcga
     5761 gagcacggcc aaccaggaca gccccctgat cgagggcaag tggccggtct tcggcctcga
     5821 cgtctgggag cacgcctact acctgaaata ccagaaccgc cgggccgact acgtgaacgc
     5881 ctggtggaac gtggtgaact gggaagtgat cagcgggcgg ctgaagtagg ggcgaccctt
     5941 gtggtcgccc tgtctgtttg cggccgacgg cggccgcgag ggccgcccct acagcgccgt
     6001 gacctgttcg aggatctgct cgtgggtggg gaactcgaac tgcgacgagg cgacgaagtg
     6061 gtagcggatg gttccggcgg cgtcgatcag gaacaacgcg ggccgggcga tgttccacgc
     6121 gtcgaggccc acgcggtgcc acaccccgta ggctttcacc gtgtcgcgcg agtcgtctat
     6181 caggatcgtg aacggcaggt tgcggtcggc gtggtagcgg cgaaccttct cggagcgctg
     6241 cgcgacgatg gccgccacct gcaccccgtg gcgcaggtac tcctcggcgt tgtgggccag
     6301 ttcaccgaac tggcgtctgc agttgggtca ccaggtgccc cggtgaaagg cgagcaacag
     6361 cggcccctgc gcgaggaact cgccgagcga cctcggcatc ccctcgtgcg tcggcagcgt
     6421 gaactccggc gccttcgccc ccagctccag cagtgattcc ctcatctcgt cgatcctgac
     6481 cgtgagacct cgtgctccag gacttgactt cggacttcgg gacttcgtgg actagtcaga
     6541 agagcttcac ctccaccacc tcccccttct cgacgatgtc cgtctgcgcc gggatttcga
     6601 tgtagccgtc ggccagcgac atactcgtga tgtcgcctga agccttgaag gccggcatcg
     6661 ccacgccgtc cacgatccgc accgtgtaga actggtgccg ccccgtcgtc gagacgaccc
     6721 tctggccgag cgggaggtgc agcgtgcggg tggcagcctc cggcaggcgt gcgaggcggc
     6781 ggaggagcgg caccagcagc atgtagccgt tcgacaggca cgaggtgggg tagcccggca
     6841 tgccgagcac gggcgtgccg ccgatgacgc cgaacagcgt cggctttccc ggcttgaccg
     6901 cgatgccgtg gaacgtcacc tcgccccggc tggcgatcag gtcgagcacc aggtcgcgct
     6961 cgccgaccga acttccgccg gagaacacga ccaggtcggt gtcgaggcac tcgtcgagcg
     7021 cgcgcgagag gtcgtcgagg cggtcggcgg aagtggcgcg cggcacgggg atcccgccgt
     7081 gctcggagac gatggccgag agggtgaagc ggttgatgtc gtagatgtgg ccgggcggga
     7141 gcggccgccc gggctcgacg atttcgttcc cggtcgagag cagggcgacg cgcggccgcc
     7201 cgaagaccgc gacctcggcg acgccgaggg cggcaatcgc gccgatccgg ctcgaggtga
     7261 ggaagtcgcc cgcgccgagg acctcctgcc cggcccggat atcggccccc tggcggccga
     7321 cgttctgccc ggggtacacc gggctgaaga tgtggacggt cccgtcctcc gcccgctcgg
     7381 tttcctcgac catcaccacc gcgtccgcgc cgccgggcat cggggcgccg gtggcgatct
     7441 gcacgcactc gccgcgcgac accgtccggg tgggcatctc gccggtgtgg accgtctcga
     7501 tcacgcgcgc cgtcttcgga tcgaactggc cggcgccgaa ggtgtcggca gccaccacgg
     7561 cgaagccgtc catggcggcg cgggcgaagg gggggacgtc ctgcgcggcg acgatggcgc
     7621 gggcgaggac gcgctggtgc gcctgctcga gcggcacggt ttcgacccgc tcgattggcc
     7681 tggcggcgcc agccatcagg gcgcgcgcct cgtcgagcgg gatcgtctcc cgtatcggac
     7741 gcatcacgcc tctcaacgcg acgcttccct caccaggtgc cccagttccg gcaggatcag
     7801 gcgcgtcatg gcgaggcgga cggcgtgttc ggagccgggc agcgagatga tcaccttgtt
     7861 cctcgcaagg ccggcgcagg cgcggctgag catcgcggcc gacccgatct cgtcgtagct
     7921 cagcatccgg aacagctcgc cgaacccgtc gagccgcttc tcgagcagcg agtcgatggc
     7981 ctcgaaggtg gtgtcgcgcg aggtgatgcc ggtgccgccc gtggtgatga tcacctggac
     8041 ggccgggttc tcgagctcgg cgaggatcgc ggcgcgcacc tggtcgggct cgtcgcgcag
     8101 cagcttgcgc cccacgacct ggtgtccccc ggcgaacagc aggtcgaaga tcgcccggcc
     8161 gctcgtgtcg gtggcctcgg tgcgggtgtc gctgatcgtg aggatgtagc agttggccac
     8221 ggcgggcgac tgcgccttgt gctcgaggtg tcccatgggg ggatcttact aggggctagg
     8281 ggccaggggc caggcgctgg caggcgagga cgccttcgga caggcgagga cggacgccgt
     8341 tcggacaggc gaggacggac gccgttcgga caggcgagga cgcctgtcct actggccgag
     8401 gtgtgcgttg atcgcgcctt ggcctgcgtc gcggaaagcg cgggagacgc cgccgacccc
     8461 gagcttcacg ccgccgaaga tgcgcgagac gaggagggcc ccctccaggt cgtgcttgcg
     8521 cagcagctcc agcagcacgc gccccgggtg ccccacctcg ccgtcgtctt tcgactgctc
     8581 gacgacggtg ccggagggat cggcgaggcg cagcgcccag cagtggtggt tggccttgcg
     8641 gtagtcgcgg cggcgcgcct tgagcagcgt ctccatctcg gcggccgacg acagcggcgt
     8701 cagcagcgcg tggaacttag agcgctcgac ggtcaaagag aaggtggagg gtgacagcac
     8761 gggacggctc atggctcaag gctcatggct cagggcagaa acacggctca cggcttgccg
     8821 cgccgaagcc gcgaac
//
LOCUS       DAOPQX010000129         8813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8922_length_8813_cov_4.819365, whole genome shotgun sequence.
ACCESSION   DAOPQX010000129 DAOPQX010000000
VERSION     DAOPQX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8813
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8922_length_8813_cov_4.819365"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKK95_08515"
     CDS             complement(<1..1076)
                     /locus_tag="PKK95_08515"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOC18295.1"
                     /translation="MKATARTVEKPAGLYWSIASEREPHPVPLEGVKVHGEILGHASR
                     VTTTQRFRNAEKVPIEAVYVFPLPEAAAVCGLAVQVGDRRIEGEVDRRDKAFDRYDDA
                     MEAGHGAVLLDEERPNVFTLSVGNILPGQEVVVELTWVAELAAEGEAIRFTLPTAVAP
                     RYSPDEDRRGVSPTPADRVSPPIASGVPYGLTLDVDVSLASPIRSVESPSHPARVEMD
                     GARARVTLSHEHAAMDRDFVLLVTPQEGGRPQVLVEKNDDGLVTAAVSFVPRLESAPA
                     PAEVVFLVDRSGSMGGSSIEQVRAALQLCLRSLRPGDCFNIVGFGSTFEALFPESVEY
                     NQATLEAAAAHVDRMDADLGGTEIL"
     gene            complement(1073..1555)
                     /locus_tag="PKK95_08520"
     CDS             complement(1073..1555)
                     /locus_tag="PKK95_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC18296.1"
                     /translation="MSVGTTYSIGELAVAAGVSRRTVRFYVQRGLLPAPEGLGRGARY
                     TDAHLARLLQIKGWQEQGVPLEEVRTRLSGSGRRAGKRAPQAPLSLERRDAGEPAQGQ
                     AWEAAATPEAPGTAWFRQPLVAGYELHVAAGRRPLTAHQLARLARALGDIVDKGGEGE
                     "
     gene            complement(1705..3558)
                     /locus_tag="PKK95_08525"
     CDS             complement(1705..3558)
                     /locus_tag="PKK95_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC18297.1"
                     /translation="MPLVVLDGVSIAYGHLPLLAGASLQVERKERIGVIGRNGTGKST
                     LLKILGGEVPPDSGTLWREPALRVARLEQDVPLSDTRPVFDVVAEGHTRHSTEDEDWV
                     REHQVDLVLSRLELDGSAVVDTLSGGWRRRVLLARALVGQPDLLLLDEPTNHLDIDTI
                     DWLEAFLAGYAGSVVFVTHDRAFLKRLATRIVEIDRGQLTSWPGDYATYLRRKEEALA
                     AEAAGQARFDRKLAEEEAWLRQGIKARRTRNEGRVRALETMRAERAARRELPGSVRLQ
                     GVVSDAATDLPGKVVFEIERVSKSYEGRPIVSGFSARVMRGDRIGLIGPNGIGKTTLL
                     RMLLGEVEPDEGEIRRGANVQIAYYDQQREQLDPERTVFETIGEGNDTVTVNGRTRHV
                     NAFLRDFLFPPERAASLVKTLSGGERNRLLLARLFTRPASVLVMDEPTNDLDIETLEL
                     VESELAEWPGTLLLVSHDRVFLDNVVTSTYVFEGGGRVQEYVGGYHDWVRQREAAAAA
                     AAAAAAAAHTRSPAGKGSSDKASRKGSPVVSTPAKPKKLSFNERRELEGLPARIEALE
                     AEQRELQARVAGPEFYKEGGAAMSEAVARLESLERELDGAYARWHELDERA"
     gene            complement(3648..4979)
                     /locus_tag="PKK95_08530"
     CDS             complement(3648..4979)
                     /locus_tag="PKK95_08530"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOC18298.1"
                     /translation="MQPENGGVGVFTHEFGHDLGLPDLYDTSGGENGTGFWTLMSSGS
                     WMDDGKDTIGNKGSHMGAWEKFQLGWLNYEVARAGEKSVHRLGPMEFNTKQAQGLFVI
                     LPPKEVVTNVGAPYAGTQYYYSGSGHNLDRLMYRSFTLGAGATLAAKVRYNIELDWDY
                     AYLVYSVDGGATWNSVQTNLSSTTNPNGQNFGDGITGVSNGWVDLTATLPAGDVLLGF
                     RYWTDPNTGGFGFQVDDLAVADFPVDGAEADAGWTYQPTPGFHVTTGVERSLANHYYV
                     AEFRQYKGYDSTLAVGPYHFGYLDSPALGNFVDHFRYQDGLLINYWDTSQTNNNTRTH
                     PGRGLLLPIDAHYAAQRRVDGAIWRNRVQSYDSTFTLEPTDGIPDIHQNSVLSPVPAL
                     PAVPVFDDRTTYYDPANPQGSVINPNTGTQIRILGVSSLGSFMQIEVRPAK"
     gene            complement(4862..5854)
                     /locus_tag="PKK95_08535"
     CDS             complement(4862..5854)
                     /locus_tag="PKK95_08535"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOC18299.1"
                     /translation="MRPCRQLWIAAAITFVGVANSAPAQPPARGDEPARIGRSDNHPD
                     PLTTRKLELKARALEAKIHGKARGRTFQVARGQYVELAREGEGAIWTVLGEFEDFAHN
                     NIAQPDRTVNNTTIWQPDFSRDYFLDLLFNDSPGANSMRNFYIEQSSGRYAVYGDVTD
                     WVRVGNHAVYDDNPDSNVWLFLRDTVNAWYAAQIAAGRTVAEINDYLSRFDVWDRYDY
                     DGDGNFDEPDGYIDTFQSVHAGEGEEAGGGALGAEAIWSHSWYAYFQGIGTGRRSTST
                     AASGSGRATTGWASTPSSRRTAAWGCSPTSSGTTWGSRISTTRPAGRTARASGR"
     gene            6307..8475
                     /locus_tag="PKK95_08540"
     CDS             6307..8475
                     /locus_tag="PKK95_08540"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOC18300.1"
                     /translation="MTRIDRLRAPLLLILAGVTAAAGLVAAPDPAGPRAVAFPPRQAA
                     AARHEARPAMGPDTILYGVAYYHEYMPSERLEKDIELMKRAGITVVRVGESTWTSWEP
                     REGEFEFAWMDRVVNAMHEAGIQVVMGTPTYSIPPWLYAKHPEIMVVPLGQVRGPQQF
                     YGPRQNMDITHPVYRQYAERVIRKIAERYAKHPGVVGWQIDNETTAYGTAGPNVQAGF
                     KEWLKRKFGTVDALNKAWGLVYWGQLLSSWDDLPPRDGILNPGWKLEWERYQRSLVTD
                     FLGWQAALVREYAPARQWVMQDFHGGARTAVDSWAISKFLDVAGMNPYHETQDALDGW
                     WIAFSGDFTRSMKRAPFLVTETNAQTIGWDAKGQFPPYDGQLRLQAWAHVAGGAHMVE
                     YWHWHSLHYGQETYWKGLLGHDLEPNRVYEEATRIGAEFKRLGPRLAGLMPRNRVAIL
                     HSADSHFGLQFMPIADRVSYLTIEDQLHKALYRLNVGADFVFAEEADFAGYDVLVVPP
                     LYVASDALLERIAAFARSGGHVLLTLKSGFTNEFDTVRATRAPGPLREACGLSYQEFS
                     SLREPLRLKGDPFGVGDEQNKASTWIDMLLPEAAKPLAFYDHPFFGRFPAITRNAFGN
                     GTVTYEGTVLTDALQSKVVASVLKGAGIEPPAGLPPRVRLREAVARDGRTLRFYLNFS
                     GEPQSFQYAHPAGTELISGRAVAPGERITLGAWDLALIEQ"
     gene            8481..>8813
                     /locus_tag="PKK95_08545"
     CDS             8481..>8813
                     /locus_tag="PKK95_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18301.1"
                     /translation="MGRAPSAARARRLGSLAVAAFLAAWTGGCASAPPEVRTPSAPSP
                     LRVTLPAAEGSVRFAVIGDGGTGKRHQVEVAGRRHRLARRAPQVAAGGAVRMVLRPER
                     IALVPPAAD"
BASE COUNT         1273 a         3087 c         3038 g         1415 t
ORIGIN      
        1 aggatctcgg tgccgcccag gtcggcatcc atccggtcca cgtgcgcggc ggccgcctcg
       61 agcgtcgcct ggttgtattc cacgctctcg gggaagaggg cctcgaaggt ggagccaaag
      121 ccgacgatgt tgaagcagtc gccggggcgc aggctgcgaa ggcagagctg gagcgccgcg
      181 cgaacctgct cgatcgacga gccgcccatc gacccggacc gatcgacgag gaacacgacc
      241 tccgcggggg cgggggccga ttcgaggcgc ggcacgaacg acaccgcggc cgtcaccagc
      301 ccgtcgtcgt tcttctccac gagcacctgc ggccggccgc cctcttgcgg cgtgaccagc
      361 aaaacgaagt cgcgatccat cgcggcgtgc tcgtgcgaga gcgtgacgcg ggcgcgcgcg
      421 ccgtccatct cgacacgcgc cgggtgcgac ggtgactcga cgctgcggat gggggaggcg
      481 agcgacacgt ccacgtcgag cgtgaggccg tacgggacgc cggacgcgat cggcgggctc
      541 acgcggtcgg ccggtgtcgg gctgacgccg cggcggtcct cgtcgggcga gtaccgcggc
      601 gcgacagcgg tgggcagcgt gaaccggatg gcctccccct cggcggccag ctcggccacc
      661 cacgtcagct cgacgaccac ctcctgcccc ggcaggatgt tgccgaccga cagggtgaag
      721 acgttcggcc gctcctcgtc cagcagcacc gcgccgtgcc ccgcctccat cgcgtcgtcg
      781 taccgatcga aggccttgtc gcgccgatcg acctcgccct cgatgcgacg gtcgcccacc
      841 tggaccgcca ggccgcacac cgctgcagcc tcgggtagcg ggaaaacgta caccgcctcg
      901 atgggaacct tctcggcgtt gcggaagcgc tgagtggtcg tgacgcggct cgcgtgaccc
      961 aggatctcgc cgtggacctt caccccctcg agggggacgg gatgcggctc gcgctccgac
     1021 gcgatgctcc agtacaagcc tgccggcttc tcgaccgtcc gggcggtggc cttcattccc
     1081 cttcgcctcc cttgtcgacg atgtctccga gggctctggc gagcctggcg agttgatgcg
     1141 cggtgagcgg gcgcctgccg gctgcgacgt gcagctcgta gccggcaacc agcggctggc
     1201 ggaaccaggc ggtcccgggg gcctccggcg tcgcggcggc ctcccatgcc tgcccctgcg
     1261 cgggttcgcc ggcgtcgcgc ctctcgaggg agagtggcgc ctgcggtgcg cgcttgccgg
     1321 cgcggcggcc gctgcccgaa agccgcgtgc ggacctcctc gagcggaacc ccctgctcct
     1381 gccacccctt gatctgcagc aggcgggcga ggtgtgcgtc ggtgtagcgg gccccgcgcc
     1441 cgaggccttc gggggccggc agcagcccgc gctgaacgta gaaccttacc gtccgccgcg
     1501 aaacgcccgc cgccactgcc agctcgccga tcgagtaggt cgtcccgacg ctcacggatg
     1561 taatataaca cttactgtcg tattaagtgt caagtttctt gtcgaggaat ggcgggcagg
     1621 caattgccgg cgccgccgcc tatcctcgcc cggctggacg agtgcgagcg gtggccgcaa
     1681 aacgtggccg gcccagggga atcgtcaggc tcgctcgtcc agctcgtgcc agcgggcgta
     1741 cgccccgtcc agctcgcgct cgagcgactc caggcgtgcg acggcctccg acatggcagc
     1801 gccgccctcc ttgtagaact cggggcctgc gacgcgcgcc tgcagctcgc gctgctcggc
     1861 ctcgagcgcc tcgatccggg cgggcaggcc ctcgagctct cgacgctcgt tgaacgagag
     1921 cttcttcggc ttggcgggcg tggagacgac ggggcttccc ttcctcgacg ccttgtcgct
     1981 gctgcccttc ccggccggcg agcgcgtatg tgctgcggcc gctgccgctg ccgcggccgc
     2041 cgctgccgcc tcgcgctggc gcacccagtc gtggtagccg cctacatatt cctgcacgcg
     2101 cccgccgccc tcgaagacgt aggtgctcgt caccacgttg tcgaggaata cgcggtcgtg
     2161 gctcacgagc agcagcgtgc ccggccactc ggccagctcg ctctctacca gctccagcgt
     2221 ctcgatgtcg agatcgttgg tcggctcgtc catcacgagg accgacgccg ggcgcgtgaa
     2281 cagccgggcg agcagcaggc ggttgcgctc gccgcccgag agcgtcttca ccagcgaggc
     2341 cgcgcgctcg ggcggaaaca ggaagtcgcg aaggaaggcg ttcacgtgcc gcgtgcgccc
     2401 gttgaccgtc accgtgtcgt tgccttcgcc gattgtctcg aagacggtgc gctcggggtc
     2461 gagctgctcg cgctgctggt cgtagtacgc gatctgcacg ttggcgccgc ggcggatctc
     2521 accctcgtct ggttcgacct ctccaagcag catccgcaac agcgtcgtct tgccgatccc
     2581 gttcggcccg atgaggccga tgcgatcacc gcgcatcacg cgcgccgaga agccgctgac
     2641 gatcgggcgt ccctcgtacg acttgctcac gcgctcgatc tcgaacacca ccttgcctgg
     2701 cagatcggtg gccgcgtccg agaccacgcc ctgcaaacgc acgctgccgg gcagttcgcg
     2761 gcgcgcggcc cgctcggcgc gcatcgtttc gagcgcccgc acccgcccct cgttcctggt
     2821 ccgccgcgcc ttgatcccct ggcgcagcca ggcctcctcc tcggccagct tcctgtcgaa
     2881 acgcgcctgc ccggccgcct cggcggcgag cgcttcctcc ttccgccgca ggtacgtcgc
     2941 gtagtcgccg ggccaggacg tcagctgccc gcggtcgatc tcgacgatgc gggtcgccag
     3001 gcgcttgagg aaggcgcgat catgcgtcac gaagacgacg gagccggcgt agccggcgag
     3061 gaacgcctcg agccagtcga tcgtgtcgat gtcgagatgg ttggtgggct cgtcgagcag
     3121 cagcagatcg ggctgcccga ccagcgcgcg cgccagcagc acgcggcgcc gccacccgcc
     3181 cgagagggtg tcgaccaccg cgctgccgtc cagctcgagc cgggacaaga cgaggtccac
     3241 ctggtgctcg cgcacccagt cctcgtcctc ggtgctgtgg cgcgtgtgcc cctcggccac
     3301 gacgtcgaag acggggcggg tgtcggagag ggggacgtcc tgctcgaggc gggcgacgcg
     3361 cagggcgggc tcccgccaga gggtgccgga gtctggcggc acttcgccgc cgagaatctt
     3421 cagcagcgtc gacttcccgg tgccgttgcg gccgatgacg ccgatccgct ctttccgttc
     3481 gacctgcagg ctcgccccgg caagcagcgg caggtggccg tacgcgatcg acaccccatc
     3541 gaggacgacg agaggcatct cagaacggta ccacggggtg cggagagggc gaataccgaa
     3601 gagcgctgca ggggcggccc tcgtgaccgc ccccgcatcg acgatttcta cttcgccggg
     3661 cgcacctcga tctgcatgaa gctgcccagc gagctgactc ccaggatgcg gatctgcgtg
     3721 cccgtgttcg ggttgatcac gctgccctgc ggattcgccg ggtcgtagta cgtggtgcga
     3781 tcgtcgaaga ccggcaccgc tggcagggcg ggcacgggcg acagcacgct gttctggtgg
     3841 atgtcgggga tcccgtcggt gggctcgagg gtgaacgtgg agtcgtagct ctggacccgg
     3901 ttccgccaga tggcgccatc gacccggcgc tgcgccgcgt agtgtgcgtc gatcggcagc
     3961 agcagaccgc ggccggggtg cgtgcgcgtg ttgttgttcg tctgcgaggt gtcccagtag
     4021 ttgatcagca gtccgtcctg gtagcggaag tggtccacga agttccccag tgccggcgaa
     4081 tccaggtatc cgaagtggta ggggcccacc gcgagcgtcg agtcgtaccc cttgtactgc
     4141 cggaactcgg cgacgtagta gtgattggcc agtgacctct ccacgcccgt ggtgacgtgg
     4201 aagccgggtg tcggctggta cgtccagccc gcgtccgcct cggccccgtc taccgggaag
     4261 tcagcgacgg ccaggtcgtc cacctggaac ccgaacccgc cggtgttcgg gtcagtccag
     4321 tagcggaagc cgagcagcac gtccccggcc ggcagggtgg cggtcaggtc cacccagccg
     4381 ttcgaaacgc ccgtgatccc gtcgccgaag ttctgcccgt tcgggttcgt cgtgctcgac
     4441 aggttcgtct ggaccgagtt ccacgtcgca ccgccgtcga cggagtagac gaggtaggcg
     4501 tagtcccagt cgagctcgat gttgtaccgc acctttgctg ccagcgtggc cccggcgccc
     4561 agcgtgaacg accggtacat caggcgatcc aggttgtgcc ccgagccgct gtagtagtac
     4621 tgggtgccgg cgtacggcgc acccacgttc gtcaccacct ccttcggcgg caggatcacg
     4681 aagagcccct gcgcctgctt ggtgttgaac tccatcggcc caagcctgtg aacgctcttc
     4741 tcgccggcgc gggccacctc gtagttcagc cacccgagct ggaacttctc ccacgctccc
     4801 atgtggctcc ccttgttccc aatcgtgtcc ttcccgtcgt ccatccagga ccccgacgac
     4861 atcagcgtcc agaagcccgt gccgttctcc ccgccggacg tgtcgtagag atccgggagc
     4921 cccaggtcgt gcccgaactc gtgggtgaac acccccacgc cgccgttctc cggctggatg
     4981 gtgtacttgc ccacccagta gtcgctctcc ccgatccgga tgccgccgta cttgttgaac
     5041 gacggcccgt gccgatgccc tggaagtagg cgtaccagct gtggctccag atggcttcgg
     5101 cgcccaacgc tccaccgccc gcctcctcgc cctcgccggc gtgcaccgac tggaacgtat
     5161 cgatgtagcc gtccggctcg tcgaagttgc cgtcgccgtc atagtcgtag cggtcccaca
     5221 cgtcgaaccg gctcaggtag tcgttgatct ccgcgaccgt cctgcccgcg gcaatctgcg
     5281 ccgcgtacca ggcgttcacg gtgtcgcgga ggaacagcca gacattggag tcggggttgt
     5341 cgtcgtagac ggcgtgattg ccgaccctca cccagtcggt cacgtcgccg tagacggcgt
     5401 agcgcccgga cgactgctcg atgtagaagt tgcgcatcga gttggcgccg ggactgtcgt
     5461 tgaacagcag gtccaggaaa tagtcgcggc tgaagtcagg ctgccagatt gtcgtgttgt
     5521 tcacggtgcg gtcaggctgc gcgatgttgt tgtgggcgaa gtcctcgaac tcgccgagca
     5581 ccgtccatat cgcgccctcc ccttcccgcg ccagttcgac gtactggcct cgcgccacct
     5641 ggaacgtccg gccccgggcc ttgccgtgga tcttggcctc cagagccctt gccttcagct
     5701 cgagcttccg ggtcgtcaag gggtctgggt ggttgtcgct gcgcccgatg cgggcgggct
     5761 cgtcgccgcg cgcgggtggc tgggccgggg cgctgttcgc caccccgacg aaagtgattg
     5821 ctgccgctat ccacagctgt cgacagggcc tcatcgtccg cctcctgctt catcgcactg
     5881 agttattggg tcgaacgttt cacaccgacg cctggaccga cgactacgag aacgaatgca
     5941 atcgcgacgg aaacaggagc gaggccccgg ggggccgcga ggattaccaa cgtccgtgcc
     6001 aaggacgcgg gcatggaaga gctgcgtggt ctaacaggcg atcggagagg gtgttggcgc
     6061 gacccgccgg cgtgagtggc gtgaaagggc gtgtagccgt tatggacagt gacgcccttg
     6121 cctgacaccg tgttctcgtc aggatcctca cgtgatgtct cgtggaatgc gaacgccgat
     6181 tgaggcccgc gagcaatggc cgcgcggcga gcccctctca ggcgtcgagc gcgttcggca
     6241 acccggcgcg gactgtatac tctccgagcc ggagggccgg cctcctggac gcacggagga
     6301 tctgccatga cccgcatcga ccgccttcgg gctcccctgc ttctgattct cgccggcgtg
     6361 acggccgcgg ccggcctcgt cgcggcgccg gaccccgccg ggccgcgcgc cgtagccttt
     6421 cctccgcgcc aggctgccgc cgcccggcac gaggcccggc cggccatggg gcccgacacc
     6481 atcctttacg gggtggcgta ctaccacgag tacatgccgt ccgagcggct cgaaaaggac
     6541 atcgagctga tgaagcgggc gggcatcacg gtggtgcgcg tgggcgagtc gacctggacc
     6601 agctgggagc cgcgcgaggg cgagttcgag ttcgcgtgga tggaccgggt cgtcaacgcg
     6661 atgcacgagg ccggcatcca ggtggtgatg gggacgccca cctactcgat cccgccgtgg
     6721 ctctacgcga agcaccccga gatcatggtc gtgccgctcg ggcaggtgcg cgggccgcag
     6781 cagttctacg ggccccggca gaacatggac atcacgcacc cggtttaccg ccagtacgcc
     6841 gagcgcgtga ttcggaagat cgccgagcgg tacgcgaagc accccggggt cgtcggctgg
     6901 cagatcgaca acgagaccac ggcgtacggc accgccgggc cgaacgtgca ggccgggttc
     6961 aaggagtggc tgaagcgcaa gttcggcacg gtcgacgcgc tcaacaaggc ctgggggctg
     7021 gtctactggg gacagctgct ctcgagctgg gacgacctgc cgccgcgcga cgggatcctc
     7081 aaccccggct ggaagctgga gtgggaacgc taccagcgct cgctcgtgac cgacttcctc
     7141 ggctggcagg cggcgctggt gcgcgagtac gcgccggcgc gccagtgggt gatgcaggac
     7201 ttccacggcg gcgcgcggac cgcggtcgat tcctgggcca tctcgaagtt cctcgacgtc
     7261 gccgggatga acccgtacca cgagacgcag gacgcgctcg acggctggtg gattgccttc
     7321 agcggcgact tcacccggtc gatgaagcgc gcgccgttcc tggtcaccga gacgaacgcg
     7381 cagacgattg gctgggacgc caagggacag ttcccgccct acgacgggca gctgcgcctc
     7441 caggcgtggg cgcacgtggc gggcggcgcc cacatggtcg agtactggca ctggcactcc
     7501 ctgcactacg gtcaggagac gtactggaag gggctgctcg gccacgacct cgagccgaac
     7561 cgggtgtacg aggaggcgac gcgcatcggc gccgagttca agcggctggg cccgcgcctc
     7621 gcggggctca tgccgcgcaa ccgggtggcg atcctgcaca gcgccgattc gcacttcggc
     7681 ctgcagttca tgccgattgc cgaccgcgtg tcgtacctga cgatcgagga ccagctgcac
     7741 aaggcgctgt accggctgaa cgtcggcgcc gacttcgtct tcgccgagga ggccgatttc
     7801 gccggctacg acgtcctcgt ggtgccgccg ctctacgtcg ccagcgacgc gctgctcgag
     7861 cggatcgccg ctttcgcccg aagcggcggc cacgtgctgc tgacgctcaa gagcggcttc
     7921 accaacgagt tcgacaccgt gcgcgccacg cgcgcgcccg ggccgctgcg cgaggcctgc
     7981 ggcctgtcgt accaggagtt ctccagcctc agggagcccc tgcggctcaa gggcgacccc
     8041 ttcggcgtcg gcgacgagca gaacaaggcc tcgacctgga tcgacatgct gctcccggag
     8101 gccgcaaagc cgctggcctt ctacgatcac ccgttcttcg gccggttccc cgccatcacg
     8161 cgcaacgcgt tcggcaacgg gaccgtgacc tacgagggaa cggtgctcac cgacgcgctc
     8221 cagtcgaagg tggtggcgtc ggtgttgaag ggggccggga tcgagccgcc ggcggggctg
     8281 ccgccgcgcg tcaggctgcg cgaggcggtg gcgcgtgacg gccggacgct gcgcttctac
     8341 ctcaacttct cgggcgagcc gcagtccttc cagtacgcgc atccggctgg gaccgagctg
     8401 atctcggggc gtgcagtggc gccgggcgag cgcattacac tgggagcatg ggatctcgcc
     8461 ctcatcgaac agtaggtggc gtggggcgcg cccccagcgc tgcccgcgcg cgccggctcg
     8521 gctcgctcgc cgttgccgcg ttcctcgccg cctggacggg cggctgcgct tcggcgccgc
     8581 ccgaggtgcg cacgccctcg gcacccagcc cgcttcgcgt cacgctgccg gccgcggaag
     8641 gctcggtgcg gttcgccgtc atcggcgacg gcggcaccgg gaagcgacac caggtcgagg
     8701 tggccggccg gcggcatcgc ctggctcggc gggccccgca ggtggcggcc gggggggccg
     8761 tccggatggt cctccggccg gagcgcatcg ccctggtccc gcccgccgcg gac
//
LOCUS       DAOPQX010000130         8783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_8962_length_8783_cov_4.252635, whole genome shotgun sequence.
ACCESSION   DAOPQX010000130 DAOPQX010000000
VERSION     DAOPQX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8962_length_8783_cov_4.252635"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /locus_tag="PKK95_08550"
     CDS             complement(<1..2148)
                     /locus_tag="PKK95_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOC18302.1"
                     /translation="MVVLSGGADPLAQGPSGRPEWDDPAVLHVGTEPPHVTMMVYPST
                     ELALSNDPARSPWFRSLNGTWKLRWSPNPAARPTDFWREDVGDEGWSDVKVPGSLETQ
                     GFGMPVYVNIGYVFPFDRQAPRPPSDDNPVASYRTRFDLPSDWVGRRVLLHFDGVDSA
                     AYVWVNGRKIGYSEGSRTPMEFDVTGAVRPGSNLLAVEVYRYSDGSFLEDQDMFRLSG
                     IFRDVYLWSPAAQHVRDFEVKTDLDARYRDATLGVRAMVVNTGKAAAAVRLDLELRDA
                     SGARVASASRRLRPAPGGGDTPADFRLQVRNPLKWTAETPSLYTLLLTLRSSGGQVLE
                     VVPSRVGFREVSIAGGKILVNGYPVLFKGVNRHEHHPVTGHHVDRETMVRDIELMKQH
                     NVNAVRTSHYPNTPLWYDLTDQYGLYVMDEANIESHGFGSNPKNRLANDPAWTPAHLD
                     RMARMVERDKNHPSVVIWSMGNEAGDGLNFAETYKWTKRRDPSRPVHYEGSSSVGGNS
                     SDINSFMYPTPKQTEELAAKRPEMPLLLCEYTHAMGNSNGGLQHYWDLFYSGRNMRGA
                     FVWDWVNQGIRQAVPAPYQMAGRPDTFLAYGGFWEDPAGQHNDWNFSQNGLISADRVP
                     YPGLRALKYVYRYLHASVIDAASGQVAVRNWFDFLNARDVASLAWSISANGRTLHAGS
                     VDLPDIPPGEQREMTLPMPAIAPEPGAECFLNLS"
     gene            complement(2224..2748)
                     /locus_tag="PKK95_08555"
     CDS             complement(2224..2748)
                     /locus_tag="PKK95_08555"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOC18303.1"
                     /translation="MRKWVAGAVLASAMLMIAPGGASGWPEGELRVADLKALEAAIAG
                     YKGQALLVNFWASWCEPCVDELPELIEVAREHRGKGVVVLTVSYDLVTSEAPRDETLK
                     EVGAFVTEQKVDLPVFLFDGPDDAGINERFGLPGAVPVTIAIDRGGKVVERHLGRATK
                     ERFGALIKRALRTR"
     gene            complement(2811..3707)
                     /locus_tag="PKK95_08560"
     CDS             complement(2811..3707)
                     /locus_tag="PKK95_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18304.1"
                     /translation="MAIGTATHMNALPPRRHGRRAGERPGFPWAAAAGLAAFGTALGV
                     LLLVLVARRTPAGTPRELLFEELQPSRLDTCALKRYGEAHDGGYLMCGNLLSAVGVGY
                     SYGINGYDGWGCDISRELGIVVHQYDCFNTTVPACPGGATTFHAECVGGRPRFDEAGR
                     RFDSVLNQLARNGDAGKRVVLKIDVEGAEWETFLSAPDAVLERIDQLAVEFHGVDDTR
                     ALPVVRRLKEHFHVAYLHFNNYSCAPDLDPFPASVYEVLFVSKRLARIDRAKTALAAL
                     TFSGRPSSPLATPSGPNRPDCQ"
     gene            complement(3868..6699)
                     /locus_tag="PKK95_08565"
     CDS             complement(3868..6699)
                     /locus_tag="PKK95_08565"
                     /inference="COORDINATES: protein motif:HMM:NF023945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOC18305.1"
                     /translation="MTPPRFLVGIDLGTTNSVVAAIDLATREAHLLPRVVPIPQVTVP
                     GEVHADAALPSFLYLPTPDERASGRFGTPWDRHPALVAGAFARDQGALQPARQVSSAK
                     SWLSHPTADRTARILPWGADEPSLSPVEASARILRHLRDAWNHEVAKGDAAAALERQS
                     VVLTVPASFDQEARELTVEAARDAGLTALTLIEEPLAAFYAFLAGAGPLRVRGAAASG
                     RQAIGESFQDGEEVLVCDVGGGTTDFTTIRVSMEEDEIRFERTAVGEHLLLGGDNLDL
                     ALARRIEARLGDRALSLVQKLALRRTVAASKERLLSHPSLDRLPVTILGSGRAIVGAS
                     VTSELTRDEVVSTLAEGFLPMAALDERPHAERRTGLLEMGLPFAADPAITRHLAAFLA
                     NAARSAGEDEPRRPDAVLFNGGFFAPALARDRIVDALARWFAPDGSWRPRVLGNPNPA
                     SAVAIGAAHYALVRERGGLRVRAGSARAYYLGLSGGGPTAESGAAICVLPRGAEEGST
                     FEVAREGLTVTTNRPVSFPLSSSALRNDRPGDLLTLPEDEIHRHAPLATVLRYGKRSR
                     QVELEVALSASYTETGTLEVSCTSRTSEHRWRLRFQLRGERDWEAPPEEGPEAESVVA
                     PGSLEAARELLRAVFAPRPAESGEVTPTARVTPETLPARLEAALGYGRLAWETPLARA
                     LADTLIELADGRRLGPRYEARWLNLLGFCLRPGFGAPADDWRIGRVRGVYVEGLLFPN
                     DPQCQSEWAVLWQRVAGGLKAGQQRELHLRYATLVGLRERKTRRVNPQVQREAWRLLA
                     SLEHLPARDRAVLGDALVGRLDRDRDNASYLWAIGRLGARVPAYGPLSAVVPPERAAP
                     WLERLMAAPVLTPERAAAIVQIGARTDDPLRDLSDDLRELAARRLAEAGFSEEAEPLL
                     TPQPAAARSALQIFGESLPPGLKVLTPPA"
     gene            complement(6696..8570)
                     /locus_tag="PKK95_08570"
     CDS             complement(6696..8570)
                     /locus_tag="PKK95_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOC18306.1"
                     /translation="MTPPGTGHGELIVGIDLGTTNCALAQVGAPGDDDRDASELRPEV
                     QPVAQVVNPGEVAERHLLPSFLYLPGDLDFAKGALALPWDPSPSRLVGELARARGAEV
                     PGRVVTSSKSWLSHAGVDRTAAILPWQAPEDVPKVSPVQASAALLEHLRHAWEQRSDG
                     RRLADQDVVLTVPASFDEDARELTRRAAAQAGLERLTLLEEPQAAFYAWIESQGDAWR
                     KRVRVGDLVLVCDIGGGTSDFTLITVAEEEGELRLERVAVGDHILLGGDNMDLALARL
                     LQQRLEAEGHRIDTWQLHALWHQARVGKERLFTDPNLPAHEVVVRGRGTRLVGGTIKT
                     MLAREDLDRVVVDGFFPPVPRDAEPARQRRVGFQELGLAYAADAAVTRHLARFLNQPA
                     GHAAGAGLVRRGPSGMACPTKILFNGGVMKAVPLRQRLVSVVNGWLEAEGFAPLAEAE
                     VLDAADLDLAVARGAAYYGRARRGRGVRIRAGVPRTYYIGIESAMPSVPGLPAPLKAL
                     CVVPFGMEEGTEAPVPGREFGLVVGEPAEFRFLSSVVRKDDPIGSLIEDWGDEVEELS
                     PLEVELDLGGREQTVVPVRLLSRITEVGTLELWCVARDGSERWKLELNVRSPRLEAGA
                     "
     gene            complement(8567..>8783)
                     /locus_tag="PKK95_08575"
     CDS             complement(8567..>8783)
                     /locus_tag="PKK95_08575"
                     /inference="COORDINATES: protein motif:HMM:NF022273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HOC18307.1"
                     /translation="EPVMAGEEGQLAVVNAGFDPGAVKLVGMVTGQPPFRGVLRHRGW
                     RVARVQLPTLPEGEGRLVIAPAEVEIG"
BASE COUNT         1264 a         3152 c         3126 g         1241 t
ORIGIN      
        1 cgagagattc aggaagcact cggctcccgg ctccggcgcg atggccggca tcggcagggt
       61 catctcccgc tgctcgcccg gcgggatgtc gggcaaatcc acgctcccgg cgtgcagcgt
      121 cctgccgttg gcgctgatgc tccacgccag ggacgccacg tcgcgcgcgt tcaggaagtc
      181 gaaccagttg cgcaccgcca cctgccccga cgcggcatcg atcaccgagg cgtggaggta
      241 gcggtagacg tacttgagcg cacgcaggcc gggatagggg acgcgatcgg cggagatcag
      301 tccgttctgg ctgaagttcc agtcgttgtg ctggccggcg gggtcctccc agaagccgcc
      361 gtacgcgagg aaggtgtcgg gcctgcccgc catctgataa ggcgccggta ctgcctgccg
      421 gatgccctgg ttcacccagt cccacacgaa ggcgccgcgc atgttgcgcc ccgagtagaa
      481 caaatcccag tagtgctgca ggcctccgtt gctgttgccc atcgcgtggg tgtactcgca
      541 gagcagcagc ggcatctcgg gccgcttggc cgccagctcc tcggtctgtt tcggtgtggg
      601 gtacatgaac gagttgatgt ccgagctgtt cccgccgacg ctgctgcttc cctcgtagtg
      661 caccgggcgc gacgggtcgc gccgcttggt ccacttgtag gtttcggcga agttgagccc
      721 gtcgcccgcc tcgttgccca tcgaccagat gacgaccgac gggtggttct tgtcgcgctc
      781 gaccatccgg gccatccggt cgaggtgcgc cggcgtccag gccggatcgt tggccaggcg
      841 gttcttcggg ttcgacccga agccgtgcga ctcgatgttc gcttcgtcca tcacgtacag
      901 gccgtactgg tcggtgaggt cgtaccagag gggggtgttc gggtagtgcg aggtgcgcac
      961 ggcgttgacg ttgtgctgct tcatcagctc gatgtcgcgc accatcgtct cgcggtcgac
     1021 gtggtggccg gtcacggggt gatgctcgtg gcggttgacc cccttgaaga gaaccgggta
     1081 gccgttgacg aggatcttcc cgccggcgat cgacacctcc cggaagccga cgcgggaggg
     1141 aacgacctcg agcacctggc cgccggacga ccgcagcgtg agcagcagcg tgtagagcga
     1201 cggggtctcg gcggtccact tgagcgggtt ccgcacctgc aggcggaagt cggccggcgt
     1261 atccccgccg ccgggcgccg gacggaggcg ccgcgacgcc gacgccacgc gcgctcccga
     1321 ggcatcgcgc agctcgaggt cgagccgcac ggcggcagct gccttgccgg tgttcaccac
     1381 catcgcgcgg acaccgaggg tcgcgtcgcg gtagcgcgcg tcgaggtccg tcttcacctc
     1441 gaagtcacgg acgtgctgcg cggcggggct ccagaggtag acgtcgcgga agatcccgct
     1501 caggcggaac atgtcctggt cctccaggaa cgacccatcg ctgtagcgat agacctcgac
     1561 ggccaggagg ttcgaaccgg gccggaccgc gcccgtcacg tcgaactcca tcggcgtccg
     1621 cgagccctcg ctgtacccga tctttctgcc gttgacccat acgtaggcgg ccgagtcgac
     1681 gccgtcgaag tgcagcagca cacgccgccc cacccagtcg gaagggagat cgaagcgggt
     1741 gcggtaggag gccacggggt tgtcgtcgct cgggggccgc ggcgcctggc ggtcgaaggg
     1801 gaagacgtag ccgatgttga cgtacaccgg catgccgaag ccctgcgtct cgaggctgcc
     1861 cggaaccttg acgtcgctcc acccctcgtc gccgacgtcc tccctccaga aatccgttgg
     1921 gcgggcggcg gggttgggcg accaccgcag cttccaggtt ccgttgagcg agcggaacca
     1981 gggcgagcgg gccgggtcgt tcgaaagcgc cagctccgtc gaggggtaca ccatcatcgt
     2041 gacgtgcggc ggctcggtgc cgacgtgcag gacggcggga tcgtcccact cggggcgccc
     2101 cgagggcccc tgcgccagcg ggtcggcacc gcccgacaga acgaccatgc agaggataac
     2161 gaccaacgat ggcagacgca tggcgtgacc tcgaaagagg ggcgagcggg ccgcaacgcc
     2221 gttctatcgc gtccgaagag cgcgcttgat cagcgccccg aaacgctcct tcgtggcgcg
     2281 gccgagatgc cgctcgacga ccttcccgcc acggtcgatg gcgatggtca ccgggacggc
     2341 gcccggcagg ccgaagcgct cgttgatgcc ggcgtcgtcg gggccgtcga agaggaagac
     2401 gggaaggtcc accttctgct cggtgacgaa cgcgccaacc tctttcagcg tctcgtcgcg
     2461 gggcgcctcg ctggtcacca ggtcgtacga cacggtcagc acgacgacgc ccttgccgcg
     2521 gtgctcgcgg gccacctcga tcagctcggg cagctcgtcc acgcacggct cgcaccacga
     2581 ggcccagaag ttcacgagca gggcctgacc cttgtagccc gcgatcgcag cctcgagcgc
     2641 cttcagatcg gcgacgcgca actcgccctc gggccaccct gacgccccgc cgggcgcgat
     2701 catcagcatg gccgacgcga gtacggcccc cgccacccac ttcctcatcg cccctccccg
     2761 cccgccgccc gcatgggtcc cgccattata ccggcctgac ttcgcgcccc ctactggcag
     2821 tcggggcgat tcggtccgct gggcgtggcc agggggctgg atggacggcc ggagaaggtg
     2881 agcgccgcga gtgcggtctt tgcgcggtcg attcgtgcca ggcgcttgct gacgaagagc
     2941 acctcgtaga ccgatgcggg gaagggatcg aggtccggtg cgcagctgta gttgttgaag
     3001 tgcaggtacg cgacgtggaa gtgctccttg aggcggcgca ccacgggcag cgcccgcgtg
     3061 tcgtccacgc catggaactc gacggccagc tggtcgatgc gctcgaggac ggcgtcgggg
     3121 gcggagagga acgtttccca ctcggcgccc tcgacgtcga tcttgagcac cacgcgcttg
     3181 ccggcgtcgc cgttgcgcgc cagttggttc agcacgctgt cgaaccggcg gcctgcttcg
     3241 tcgaagcggg gccggccgcc cacgcactcc gcgtggaagg tcgtggcgcc gcccgggcag
     3301 gccgggacgg tggtgttgaa gcagtcgtac tggtggacga cgatgcccag ctcgcgcgag
     3361 atgtcgcagc cccagccgtc gtacccgttg atgccgtatg agtagccgac gcccaccgcc
     3421 gagagcaggt tcccgcacat caggtagccg ccatcgtgcg cctcgccgta ccgcttcaag
     3481 gcacaggtgt cgagccggga cggctgcagt tcctcgaaca gcagctctcg cggcgtcccg
     3541 gccggcgtcc ggcgggcgac gaggacgagg aggaggacgc cgagggcagt gccgaacgcc
     3601 gccagccctg cggcggccgc ccacgggaag cctggccgtt cgccggcgcg acgcccgtgc
     3661 cgccggggag gaagcgcgtt catgtgtgtt gccgtcccga tggccatgct accattcgcc
     3721 gccccgatcc ccacgcgggg atcgccctcc acgacctgcc cgtctccccg ttgtcggtgt
     3781 cttggccgga aggagcgacg acatggtgat caagagcgtc ggtgcgggct cgcggtgcag
     3841 ccgctggaac tgactgagac ggagtggtca ggcagggggt gtgagcacct tgaggccggg
     3901 cggcagcgac tccccgaaaa tctgcagcgc gctgcgcgcg gccgcgggct gcggcgtcag
     3961 cagcggctcg gcttcctctg agaacccggc ctcggccagc cgccgggccg cgagttcgcg
     4021 caggtcgtcg ctgagatcgc gcagcgggtc gtcggtccgg gcgccgatct ggacaatcgc
     4081 cgcggcccgc tcgggcgtca ggacgggcgc ggccatcagg cgctcgagcc agggcgcggc
     4141 gcgttcgggc ggtacgacgg ccgacagcgg gccgtacgcc gggacccgcg cgcccagccg
     4201 gccgatcgcc cacaggtagc tcgcgttatc gcggtcacgg tcgaggcgac cgacgagcgc
     4261 atcgccgagc accgctcgat cgcgcgcggg aaggtgttcg aggcttgcca gcagccgcca
     4321 ggcctcgcgc tgcacctggg gattcacccg cctggtcttg cgttcccgca ggccgacgag
     4381 cgtcgcgtag cgaaggtgca gctcgcgctg ctggcccgcc ttgaggccgc cggccacgcg
     4441 ctgccacagc acggcccact ccgactggca ctgcgggtcg ttcgggaaca gcagcccctc
     4501 gacgtacacg cctcgcacgc ggccgatgcg ccagtcgtcg gccggcgcgc cgaacccggg
     4561 ccgcaggcag aagccgagca ggttgagcca tcgcgcctcg tagcgcgggc caaggcgtcg
     4621 gccgtcggcc agctcgatca gcgtgtcggc gagggcgcgg gccagcgggg tctcccacgc
     4681 caggcgtccg tacccgagcg ccgcctcgag ccgggcgggc agcgtctcgg gagtgacgcg
     4741 ggcggtgggt gtcacttcgc cagattcggc aggccggggc gcgaagacgg ctctcaggag
     4801 ctcgcgcgcg gcttccaggc tcccgggcgc gaccaccgac tcggcttcgg gcccctcctc
     4861 cgggggcgct tcccagtcgc gctcgcctct cagctgaaag cggagccgcc accggtgctc
     4921 gctggtcctc gacgtgcacg acacctcgag cgtccccgtc tccgtgtagc tcgccgagag
     4981 cgcaacctcc agctcgactt gccgcgaccg ctttccgtac cggagcacgg tggcgagcgg
     5041 ggcgtgccga tggatctcgt cttccggaag cgtgaggagg tcgcccggcc gatcgttccg
     5101 caacgcgctg ctcgagagcg ggaacgagac gggccggttg gtcgtgaccg tgagcccctc
     5161 gcgcgcgacc tcgaaggtgg aaccctcctc ggcgccgcgc ggcagcacgc agatggcggc
     5221 accgctctcc gccgtcggcc ctccgcccga caagcccagg tagtacgccc gcgcgctgcc
     5281 cgcacgaacc ctcaggccgc cgcgctcgcg cacgagcgcg tagtgggcag cgccaatcgc
     5341 gacggccgac gccgggttcg ggttcccaag cacccgcggc cgccacgatc cgtccggcgc
     5401 gaaccagcgg gccagcgcgt ccacgatccg gtcgcgggcg agggccggag cgaagaagcc
     5461 gccgttgaac aggacggcgt cgggcctgcg cggctcatcc tcgcccgcag atcgcgccgc
     5521 gttcgcgagg aacgcggcca ggtggcgcgt gatcgccggg tcggccgcga acggaaggcc
     5581 catctccagc aggcccgtcc gccgctcggc gtgcggccgc tcgtcgagcg ccgccatcgg
     5641 caggaagcct tcggcgagcg tcgagaccac ttcgtcgcgg gtcagctccg aggtgacgga
     5701 ggccccgaca atcgcgcggc cgctcccgag gatcgtgacc ggcagccgat cgagtgacgg
     5761 gtgggagaga aggcgctcct tcgacgcggc gaccgtgcgc cggagcgcaa gcttctggac
     5821 cagcgagagg gcgcggtcgc cgagccgggc ctcgattcgc cgcgcgaggg cgaggtcgag
     5881 gttgtcgccg ccgagcagca ggtgctcacc cacggccgtc cgctcgaagc ggatctcgtc
     5941 ttcttccatc gacacgcgga tggtggtgaa gtcggtcgtg cccccgccga cgtcgcagac
     6001 cagtacttct tcgccatcct ggaacgactc accgattgcc tgtcttcccg aagcggccgc
     6061 gccgcgaacg cggaggggac cggcgccggc caggaaggcg tagaacgcgg ccagcggctc
     6121 ctcgatcagc gtcagcgccg tcagccccgc gtcgcgagcc gcctctacgg tcagctcgcg
     6181 cgcctcctgg tcgaatgacg ccggcacggt gagcacgacg ctctggcgct cgagcgcggc
     6241 ggcggcgtcc cccttcgcca cttcgtggtt ccacgcgtcg cgcaggtgcc gcaggatgcg
     6301 ggccgaggcc tccaccggtg agagcgacgg ctcgtcggcc ccccatggca ggatgcgagc
     6361 cgtccggtcg gccgtcggat gcgacagcca cgacttcgcc gacgagactt gccgtgcggg
     6421 ctgcagcgcc ccctggtcgc gcgcgaacgc gccggccacc agggcgggat ggcgatccca
     6481 tggcgtgccg aatcggccgc tggcgcgctc gtcgggtgtt ggcaggtaga ggaaggaggg
     6541 aagcgcggcg tcggcatgca cctcgcccgg cacggtgacc tgcggtatcg gcacgacccg
     6601 gggcaggagg tgcgcctcgc gggtggccag gtcgattgcg gcgacaaccg agttggtggt
     6661 gccgagatcg atgccgacga ggaagcgggg cggcgtcatg cccccgcttc gagacgcggc
     6721 gagcgcacgt tcagctcgag cttccagcgc tcggagccgt cgcgtgcgac gcaccacagc
     6781 tccagcgtcc cgacctccgt gatgcggctc agcagccgca ccggcacgac cgtttgttcg
     6841 cggcccccca ggtcgagctc cacctcgagc gggctcagtt cctcgacctc gtcgccccag
     6901 tcctcgatca ggctgccgat cgggtcgtcc ttccgcacca ccgacgacag gaaccggaac
     6961 tccgccggct ctcccaccac gagcccgaac tcgcgcccgg gcaccggggc ctcggttccc
     7021 tcctccatgc cgaaggggac gacgcagagc gccttcagcg gggcagggag gcccggcacc
     7081 gagggcatcg cgctctcgat gccgatgtag taggtccgcg ggacccccgc gcggatgcgg
     7141 acgccccgtc cgcgccgcgc ccgcccgtag tacgcggcgc ctcgcgccac ggccaggtcg
     7201 aggtcggccg cgtcgagcac ctcggcctcg gcgagcggcg caaacccctc ggcctcgagc
     7261 cagccgttga ccaccgaaac cagccgctgc cgcagcggca cagccttcat cacgccgccg
     7321 ttgaacagga ttttcgtcgg gcacgccatc ccgctcgggc cgcggcgcac caggccggcg
     7381 cccgccgcgt gcccggccgg ctggttgagg aaccgggcga ggtggcgggt gacggcggcg
     7441 tcagcggcat acgcaaggcc cagttcctgg aagcccacgc gccgctgccg ggccggttcg
     7501 gcgtcccggg gtaccggggg gaagaacccg tccaccacca cccggtcgag atcctcgcgc
     7561 gccagcatcg tcttgatcgt cccgcccacc aggcgcgtcc cgcgcccgcg gacgacaacc
     7621 tcgtgcgcgg gcagattggg gtcggtgaac agacgttcct tgcccacgcg ggcctggtgc
     7681 cagagcgcgt gcagctgcca ggtgtcgatc cggtggccct cggcctcgag gcgctgctgc
     7741 agcagccgcg cgagcgccag gtccatgttg tcgccgccga gcaggatgtg atcgcccacc
     7801 gcgacgcgct cgaggcgcag ttcgccctct tcctccgcga ccgtgatcag cgtgaagtcg
     7861 ctggtgccgc cgccgatgtc gcacaccagc acgaggtcgc ccacgcgtac ccgctttcgc
     7921 cacgcgtcgc cctggctctc gatccaggcg tagaaggcgg cctgcggctc ctcgagcagg
     7981 gtgagccgct cgagccccgc ctgcgccgcg gcgcggcgcg tcagctcgcg cgcgtcctcg
     8041 tcgaaggagg cggggaccgt caggacgacg tcctggtcgg cgaggcggcg gccatccgac
     8101 cgctgctccc aggcgtgccg caggtgctcg aggagggcgg ccgacgcctg caccggcgag
     8161 accttgggca cgtcctcggg cgcctgccac gggaggatcg cggccgtgcg atccacgccg
     8221 gcgtgcgaga gccacgattt cgacgaggtc acgacgcgcc cgggcacctc ggctccccgt
     8281 gcccgcgcca gttcgccaac caggcgcgag ggcgacgggt cccacggcag cgcgagagcg
     8341 cccttcgcga aatcgagatc gccggggaga tagaggaagg aaggcagcag gtgacgctcg
     8401 gccacctcgc ccgggttcac tacctgcgcc accggctgca cctccgggcg cagttcgctg
     8461 gcatcgcggt cgtcgtcgcc gggcgcaccg acctgcgcga gggcgcagtt ggtcgtgccg
     8521 aggtcgatgc cgacgatcag ttcgccgtgt ccggtgccgg ggggcgtcat ccgatctcca
     8581 cctctgcggg tgcgatgacc agccgcccct cgccctcagg gagggtgggc agctgcacgc
     8641 gggccacgcg ccagccgcgg tggcgcaaca ccccgcggaa gggaggctgg ccggtgacca
     8701 tccccaccag cttcacggcg ccaggatcga aaccggcgtt caccacggca agctgcccct
     8761 cctcgcccgc catcaccggt tcc
//
LOCUS       DAOPQX010000131         8731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9017_length_8731_cov_4.574228, whole genome shotgun sequence.
ACCESSION   DAOPQX010000131 DAOPQX010000000
VERSION     DAOPQX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9017_length_8731_cov_4.574228"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKK95_08580"
     CDS             complement(<1..411)
                     /locus_tag="PKK95_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     N-terminal domain-containing protein"
                     /protein_id="HOC18308.1"
                     /translation="MAIKVGINGFGRIGRNIMRAALGNSEFDFVAVNDLTSAQTLAHL
                     LKYDSVLGNITAKVEAKDGAITVDGDEFKVLSAKDPAELPWKELGVDIVFEATGKFTK
                     RDDCAKHLAAGAKKVIVTAPAKGPDATLVMGVNEG"
     gene            595..1932
                     /locus_tag="PKK95_08585"
     CDS             595..1932
                     /locus_tag="PKK95_08585"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOC18309.1"
                     /translation="MYFLYTCLSLVAFVLLSPYFAWQALRYHKYVANLRQRLGYLPVS
                     FNLDADESIWIHAVSVGEALSVRAFAGELKSRYPGLRLFVSTTTIAGQQVAARDITEA
                     DATFYFPIDLGFIVRRTLRLVKPRLLVVMETELWPNLLRECKRRGVRTMLVNGRISNR
                     SYPRYRLVRPFMRRVLADIDRFCVQSFEAARRLTDLGADPGLVEVTGNLKFDSLKAGP
                     APDERGVHRVLRYFQVPEGRPVVVAGSTMRGEETMVLQAYERLRTPERRPLLVIAPRH
                     PERFDEVERLAREEGLAVERRTRLDIDVEVRADVVVLDTIGELAHLYRIAWVVFVGGS
                     LVPTGGHNILEPAVFGKPIVFGPYMHNFREIADSFLAADAALQVASPSELADVLEDLL
                     DDEKRRARLGAAARGIIESSRGARDRTLAAIAAVLPPGTGARVRPFRPPRARS"
     gene            1932..2972
                     /gene="lpxK"
                     /locus_tag="PKK95_08590"
     CDS             1932..2972
                     /gene="lpxK"
                     /locus_tag="PKK95_08590"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOC18310.1"
                     /translation="MLDHLYAAAALARRRWYERRPERRRRLDRPVVSVGNVSVGGSGK
                     TPVTACLARLLLDAGHVPSILTRGYARRDPAPGVTVVSDGKQLRAGIDQAGDEPLMLA
                     RALPAAPVLVSSDRYLAGQLAERRFGCTVHLLDDGFQHFGLHRDVDLVLVGQEELESP
                     VALPRGRLREPIDVLRSADALVFEMVDAALPVALALAERVGVTRCFRLRRALGRPRLA
                     GGDAATAPAEGTRVLAVAGLARPQGFFSALAVEGWTLAGQLAFPDHHRFGEADVRRIL
                     GAMREARAGMVVTTEKDWVRLQPLEPLPFTVALAPLEVTVEPPDTFREWLLASVGQAV
                     GQASRLSRNREP"
     gene            3042..4004
                     /locus_tag="PKK95_08595"
     CDS             3042..4004
                     /locus_tag="PKK95_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC18311.1"
                     /translation="MSPEPVFRHRLEYAVVAAVALVIRVLPWGAVRLAGGFLGTLFYL
                     FDRQHRRVATDNLARAFPTRTASEIRALAHGVFRHFGGMLFELLKFSTLRHDRMLERV
                     EFEGTDRVREAYAGRRGVFFLTGHFGCWEINGIVHALHVEPIGVMARRLDNPYLNALL
                     ERVRQCTGNWVIYRKGGLRRMLRALEAGRGIAILIDQHMHGPEAVPVDFFNRPAATTT
                     VLGSLALRTGAPVIPVFTVPLAGGRYRVIYEHAVEPPSEASPEALRAFTQRCTDVLEM
                     YVRRYPTQWLWMHRRWRDRPADDVVKGMFPQARSEGDIEGRVPE"
     gene            4004..5053
                     /gene="waaF"
                     /locus_tag="PKK95_08600"
     CDS             4004..5053
                     /gene="waaF"
                     /locus_tag="PKK95_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOC18312.1"
                     /translation="MARRTLVLAPNWLGDAVMALPALAGLRRQHPGDHLIAAARPSIA
                     PLFEMVGGIDQVVRLPAGRAWHPADLRREAAAIASATADLAILLTNSFRTAWIVRRAG
                     VAERWGVRADLRGRLLTRAVPRPRRRGAHHADYYRDIVLAFGIEPARESPRVTPPPAA
                     FETARVLLERESVPAGAPLVGMAPGAAYGKAKQWPPRRFAELAAELHDRFGAVSVLVG
                     SGGDREAGEEIRRCLDGAAARASLVNLIGKTDLVVLAGVIARMRAFVSNDSGAMHLAA
                     AVGLPVAAVFGPTDERATAPLPAPAHGVPAHAILTAPAWCRPCLLRECPIDHRCMTRI
                     SAAAVTGAMEGWFER"
     gene            5050..5640
                     /locus_tag="PKK95_08605"
     CDS             5050..5640
                     /locus_tag="PKK95_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC18313.1"
                     /translation="MSAAVFLDRDGTIIEEYGYLNRLDRVTFFPWSIDAIRALNAAGL
                     LVFVVTNQAGVARGYYDEAFVKATHALIDKRVRAGRARIDAFYYCPHHPDGSVAAYRT
                     VCECRKPAPGMIRRAALEYGVDAARSFVVGDRWLDVEFGRAAGARSILVRTGYGLNEE
                     SRPANAPRADLVADNLAEAVAWILGVASASVPANRP"
     gene            5637..6644
                     /locus_tag="PKK95_08610"
     CDS             5637..6644
                     /locus_tag="PKK95_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOC18314.1"
                     /translation="MTSDRLLSLVDRFPSCRVVVAGDLVADEFLSGQPSRVSREAPVL
                     ILSYEDTRVVPGGGANAANNVATLGGRVQLFGLTGRDEAGRRLLECFPRSVNTRNVLR
                     PAGYRTPTKTRVLAGGIHSAKQQIVRIDREPGAPPPPELAARIADALLEAAGAADAVL
                     LSDYGYGLVTPALASAITARLEAARRDARPPVLIDSRYNLGAYRGLAACTPNQPEVEQ
                     LFDRKIGDDGAALEKAGRTLLRRLQMQAVLVTRGSRGMALFQRSVPTVHIPIFGTDEV
                     ADVTGAGDTVMAVMSLALAAGASFHEAALLANYAGGIVVMKRGTATVSPDELREAIRS
                     Q"
     gene            6774..7292
                     /locus_tag="PKK95_08615"
     CDS             6774..7292
                     /locus_tag="PKK95_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOC18315.1"
                     /translation="MGQILSEPDLQAQIAADRREGRTVAFANGCFDVLHVGHVRYIQG
                     AAAEADRLIVAVNDDGSVRRMKGANRPVLPAADRAELVAALRGVDYVVVFPDATVARL
                     LELLKPDVHCKGTDYTVDTVPERETVRAYGGRIAIVGDAKEHSTSELLGRIKAEGGPP
                     SRQAPRRGRPEP"
     gene            7323..8417
                     /locus_tag="PKK95_08620"
     CDS             7323..8417
                     /locus_tag="PKK95_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC18316.1"
                     /translation="MRVLLVRLGAMGDIVHALPVAAALRQRFPFARIDWVVDARYAAL
                     LDLVPAIDGRIVVAARDQAPAGAGRAPGSPGRDGVADGPTGDRARRTFSAGLGWLRLV
                     RALRRERYEVALDLQGLVKSAVVARASGARRVIGFGRPWLREPLAARFYTERTAVARG
                     GHVVDKNLALLSALGIGTAARVFPLAAGDMRPVEEMRRLHPEAARGFVLLNPGAAWPN
                     KRWPPERFGALAAIVRRRHGLPSAVLWGPGEEELAALVVDESGGAAFAAPATRLPGLL
                     GLARSARLIVSGDTGPLHLAAAAGTPAVAVFGPTNPERNGPWHPADLTVSRYDTCDCH
                     YRRQCRRGRPCIAEIEVDEVARAVDLRLAT"
     gene            8463..>8731
                     /locus_tag="PKK95_08625"
     CDS             8463..>8731
                     /locus_tag="PKK95_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18317.1"
                     /translation="MTSSTSWTSWTARGARLLARTRVPLGFLGGVVALWLAHPTWRSL
                     AAGAAVAALGEAIRVWAAGHLEKGREVTRSGPYRWTAHPLYLGSA"
BASE COUNT         1090 a         3129 c         3178 g         1334 t
ORIGIN      
        1 cccctcgttc acacccatca cgagcgtcgc gtccgggccc ttggccggcg cggtcacgat
       61 gaccttcttc gccccggcgg ccaggtgctt ggcacagtcg tcgcgcttgg tgaacttgcc
      121 ggtcgcctcg aagacgatgt cgacccccag ctccttccac ggcagctcgg ccgggtcctt
      181 ggcggagagc accttgaact cgtcgccgtc gaccgtgatg gccccgtcct tcgcctcgac
      241 cttggctgtg atgttcccga gcacggagtc gtacttgagg aggtgcgcga gcgtctgggc
      301 gctcgtcagg tcgttcaccg ccacgaagtc gaactcacta ttgccgaggg ccgcgcgcat
      361 gatgttgcgg ccgatccgac cgaacccgtt gatcccgacc ttgatggcca tcgattcctc
      421 cagcgagttg attgcggcga gctaggcgcc cgttctttcc gtcagtaaaa ccaaaccatt
      481 ctaatgacgg cttgcgggag ggtcaaacac ggactatact gccgcgtttt ccaggaattc
      541 cggccgcccg ccccagcggc cggccagccg cccgacaccc gtcgccccgg cccgatgtac
      601 ttcctgtaca cgtgtctcag cctcgtggcc ttcgtcctgc tgtcgccgta cttcgcgtgg
      661 caggcgctcc gctaccacaa gtacgtcgcc aacctccgtc agcgcctcgg ctacctcccg
      721 gtctccttca acctcgacgc cgacgagtcg atctggatcc acgccgtgtc ggtgggcgag
      781 gcgctctcgg tgcgcgcgtt cgccggcgag ctgaagtccc gctatccagg cctccggctg
      841 ttcgtctcca ccaccacgat tgccggccag caggtggccg cccgcgacat caccgaggcc
      901 gacgccacct tctacttccc gatcgacctc ggtttcatcg tccgccgcac gctgaggctg
      961 gtgaagcccc ggctgctcgt cgtgatggag accgagttgt ggccgaacct cctgcgcgag
     1021 tgcaaacgcc gcggcgttcg gaccatgctc gtcaacgggc gcatctcgaa ccgctcgtac
     1081 ccccgctacc gcctcgttcg cccgttcatg cgccgcgtgc tcgccgacat cgaccggttc
     1141 tgcgtccagt cgttcgaggc ggcgcgccgg ttgaccgacc tcggcgccga ccccggcctc
     1201 gtcgaggtga ccggcaacct gaagttcgac tcgctcaagg caggcccggc gcccgacgag
     1261 cgcggcgttc accgcgtgct ccgctacttc caggtgcccg aggggcgtcc ggtcgtcgtg
     1321 gccggctcga cgatgagggg cgaggagacg atggtgctgc aggcgtacga gcgcctgcgg
     1381 acgcccgagc gccggccgct gctcgtgatc gcgccccgcc accccgagcg gttcgacgag
     1441 gtcgagcggc tggcgcgcga ggaagggctg gcggtcgaac ggcggacgcg cctcgacatc
     1501 gacgtcgagg tgcgggccga cgtggtcgtg ctcgacacca tcggcgagct ggcgcacctc
     1561 taccgcatcg cctgggtggt gttcgtcggg gggagcctgg tgccgacggg gggccacaac
     1621 atcctcgagc cggcggtgtt cggcaagccg atcgtgttcg gcccctacat gcacaacttt
     1681 cgcgagatcg ccgacagctt cctcgccgcc gacgccgcgc tccaggtggc ctcgcccagc
     1741 gagctggccg acgtgctgga agacctgctg gacgacgaga agcggagggc gcgcctgggg
     1801 gccgccgccc gtggcatcat cgagtcgagc cgcggcgcgc gcgatcgcac cctggccgca
     1861 atcgccgccg tcctgccccc cggcaccggc gcccgggtgc ggccgttccg cccgccccgg
     1921 gcccgatcgt gatgctcgac cacctctacg ccgccgccgc cctcgcccgg cgccgctggt
     1981 acgagcggcg gccggaacgc cgccgccggc tcgaccggcc cgtggtgagc gtcgggaacg
     2041 tcagcgtcgg cggtagcggg aagacgccgg tgaccgcctg cctggcgcgc ctgctgctcg
     2101 acgccggcca cgtcccctcg atcctcacgc gcggctacgc gcggcgcgac ccggcgcccg
     2161 gggtcaccgt ggtcagcgac ggcaagcagc tgcgggccgg catcgaccag gccggcgacg
     2221 agccgctgat gctggcccgc gcgctgccgg cggcccccgt gctggtgtcg agcgaccgct
     2281 accttgccgg acagctggcc gagcgccggt tcggctgcac ggtccacttg ctggacgacg
     2341 ggttccagca cttcgggctg caccgcgacg tggacctggt gctggtcgga caggaggagc
     2401 tggagagccc cgtggcgctg ccgcgcgggc ggctgcgcga gccgatcgac gtgctccgct
     2461 cggccgacgc gctggtcttc gagatggtgg acgccgcgct gcccgtcgca cttgccttgg
     2521 ccgagcgggt cggcgtgacg cggtgtttcc gcctgcgtcg cgcgctgggg cggccgcggc
     2581 tggccggcgg cgacgcggcg accgcgccgg ccgagggcac gcgggtgctg gcggtggcgg
     2641 gcctggcaag gccccagggg ttcttctcgg cgctggccgt cgaggggtgg acgcttgccg
     2701 gccagctggc gtttcccgat caccaccgct tcggcgaggc cgacgtccgc cgcatcctcg
     2761 gcgccatgcg cgaggcccgg gccggcatgg tggtgacgac cgagaaggat tgggtgcgcc
     2821 tgcagccgct cgagccgctg cccttcaccg tcgccctcgc gccgctcgag gtgaccgtgg
     2881 agccccccga cacgttccgc gagtggctgc tcgcttcagt gggacaggca gtgggacagg
     2941 cgtctcgcct gtccaggaac cgtgagccgt gagccttgag tcatgagcgg tgttcagtgg
     3001 gacaggcgtc ccgcctgtcc aggaaccgtg agccgtgagc cgtgagccct gagcccgtct
     3061 tccgtcatcg cctcgagtac gcggtcgtgg cagccgtcgc gctggtgatc cgcgtgctgc
     3121 cctggggggc ggtgcgcctg gccggcgggt ttctcgggac gttgttctac ctgttcgacc
     3181 ggcagcaccg ccgggtggcg accgacaacc tcgcgcgcgc cttcccgacc cgcaccgcct
     3241 cggagatccg ggcgctggcg cacggcgtgt tccgccactt cggggggatg ctcttcgagc
     3301 tgctgaagtt cagcaccctg cggcacgatc ggatgctcga gcgggtcgag ttcgagggga
     3361 ccgaccgggt gcgcgaggcc tacgcgggcc ggcgcggcgt cttcttcctg actggccact
     3421 tcggctgctg ggagatcaac ggcatcgtcc acgcgctgca cgtcgagccg atcggcgtga
     3481 tggcgcggcg tctcgacaac ccctatctga acgccctgct cgagcgcgtg cggcagtgca
     3541 ccggcaactg ggtgatctac cggaagggcg ggctgcggcg gatgctgcgg gccctcgagg
     3601 cgggccgggg gatcgcgatc ctgatcgacc agcacatgca cggccccgag gcggtgccgg
     3661 tggacttctt caaccggccc gccgccacca ccaccgtgct cggatcgctc gcgctgcgga
     3721 cgggggcgcc ggtgatcccg gtgttcacgg tgcccctcgc cgggggccgc taccgcgtga
     3781 tctacgagca tgccgtcgag ccgccgtccg aggcgtcgcc cgaggccctg cgcgccttca
     3841 cccagcgctg caccgacgtc ctggagatgt acgtgcggcg ctaccccacg cagtggctct
     3901 ggatgcaccg ccgctggcgt gaccgccctg cagacgacgt ggtgaagggg atgttcccgc
     3961 aggcccggtc ggaaggcgac atcgagggcc gggtgccgga gtgatggccc gccgcacgct
     4021 ggtcctcgcg ccgaactggc tcggcgacgc cgtgatggcg ctgccggccc tggcggggct
     4081 ccggcggcag caccccggcg accacctgat cgccgcggca aggccttcaa tcgcgcccct
     4141 cttcgagatg gtcggcggga tcgaccaggt ggtgcggctg ccggcaggac gcgcctggca
     4201 tccggccgac ctgcggcgcg aggcggcggc gattgcctcg gcgacggccg acctcgccat
     4261 cctcctgacc aactccttcc gaaccgcttg gatcgtgcgc cgggccggcg tcgcggagcg
     4321 gtggggcgtg cgggccgacc tgcgtggccg gctgctcacg cgcgcggtgc caaggccgcg
     4381 acggcggggc gcgcaccacg ccgactacta ccgcgacatc gtcttggcgt tcggcatcga
     4441 gccggcgcga gagtcccctc gtgtcactcc tccgccggcg gcgttcgaga ccgcgagggt
     4501 gctgctcgag agggagagcg tcccggccgg ggcgccgctg gtgggcatgg cgccgggggc
     4561 ggcttacggg aaggcgaagc agtggccgcc ccgccgcttc gccgagctgg ccgcggaact
     4621 gcacgatcgg ttcggcgcgg tctcggtgct ggtgggcagc ggcggggatc gcgaggctgg
     4681 ggaagaaatt cgccggtgcc tcgacggcgc ggcggcgcgc gcgagcctcg tcaacctgat
     4741 tggaaagacc gacctggtgg tgctggccgg ggtcatcgcc cggatgcggg cgttcgtgtc
     4801 gaacgattcg ggggcgatgc acctggctgc ggcggtcggc ttgccggtgg ccgcggtgtt
     4861 cgggccgacc gacgagcggg ccaccgcacc gctgcccgcg ccggcgcacg gcgtgccggc
     4921 gcatgcgata ctgacggcgc ccgcctggtg ccgcccctgc ctgctgcggg agtgccccat
     4981 cgaccaccgc tgcatgacgc gaatctccgc ggcggcggtg accggggcga tggaagggtg
     5041 gttcgagaga tgagcgcggc ggtgttcctg gatcgcgacg ggacgatcat cgaggagtac
     5101 gggtacctga accggctcga tcgggtgact ttcttcccct ggagcatcga cgcgattcgt
     5161 gcgctgaacg ccgcgggcct gctcgtcttc gtcgtcacca accaggccgg cgtcgcgcgc
     5221 ggctactacg acgaggcatt cgtgaaggcg acgcacgccc tcatcgacaa gcgggtccga
     5281 gcggggcggg cgcgcatcga cgccttctac tactgcccgc accacccgga cggaagcgtg
     5341 gcggcctacc gcaccgtctg cgagtgccgg aagcccgcac ccggcatgat ccgccgcgct
     5401 gcgctcgagt acggcgtcga cgcggcacgc tcgttcgtgg tcggagaccg ctggctcgac
     5461 gtcgagttcg ggcgcgcggc cggcgcgcgg agcatcctcg tgcggaccgg gtacggcttg
     5521 aacgaggagt cgcgccccgc caacgcgccg cgcgccgacc tggtggccga caacctggcg
     5581 gaagcggtgg cgtggatact gggagtagcg tcggcctccg tgccggcgaa ccgaccatga
     5641 catcagatcg tctgctctct ctcgtcgatc gcttcccctc ctgccgggtg gtggtggcgg
     5701 gcgacctcgt ggccgacgag ttcctgtcgg ggcagccgtc gcgcgtgtcg cgcgaggcgc
     5761 ccgtgctgat cctcagctac gaggacacgc gggtcgtgcc cggcggcggc gccaacgcgg
     5821 cgaacaacgt ggccacgctc ggcggccgcg tccagttgtt cggcctcacc ggccgcgacg
     5881 aggccggccg ccggctgctc gagtgcttcc cgcgctcggt caacacgcga aacgtcctgc
     5941 gcccggcggg ctaccgcacg cccaccaaga cgcgcgtgct tgccggcggc atccactcgg
     6001 cgaagcagca gatcgtgcgg atcgaccggg agcccggcgc gccgccgcct cccgagcttg
     6061 ccgcgcggat tgccgacgcc cttctcgaag cggccggcgc cgccgacgcg gtgctgctct
     6121 ccgactacgg ctacggcctg gtgacgccgg cgctcgcctc ggccatcacc gcgcggctcg
     6181 aggctgcccg ccgcgacgcg cggcctccgg tgctgatcga ttcgcggtac aacctcggcg
     6241 cctaccgcgg ccttgccgcg tgcactccaa accagccgga agtggagcag ctgttcgaca
     6301 ggaagattgg cgacgacggc gcggcgctcg agaaggcggg gcggacactg ctgcggcggc
     6361 tgcagatgca ggcggtgctg gtgacgcgcg gctcgcgggg catggcgctc ttccagcggt
     6421 cggtgccgac cgtccacatc ccgatcttcg gaaccgacga ggtggccgac gtcacgggcg
     6481 ccggcgacac ggtgatggcg gtcatgtcgc tcgcccttgc cgccggcgcg tcgttccacg
     6541 aggcggcgct gctggccaac tacgccggcg gcatcgtggt gatgaagcgc ggcaccgcca
     6601 ccgtctcccc cgacgagctg cgcgaggcga ttcggagcca gtagccaggc gtctcgcctg
     6661 tgagtggcag ccagtagcac aggcgtctcg cctgtgagtg gcagccagta gcacaggcgt
     6721 ctcgcctgtg tgtacaacag cagtggcaca ggcgtcccgc ctgtgagtga cagatgggac
     6781 agatcctctc cgaacccgat ctccaggcgc agatcgccgc cgatcgccgc gagggccgca
     6841 ccgtcgcgtt cgccaacggc tgcttcgacg tgctgcacgt cggccacgtg cggtacatcc
     6901 agggagccgc cgccgaggcc gaccgcctga tcgtggccgt caatgacgac ggttcggtga
     6961 ggaggatgaa gggggcgaac cgccccgtgc tgccggcggc tgaccgtgcc gagctggtgg
     7021 ccgcgctgcg cggcgtcgat tacgtcgtgg tcttccccga cgccaccgtg gcgcgcctgc
     7081 tcgagctgct gaagcccgac gtccactgca aggggaccga ttacacggtg gacaccgtgc
     7141 ccgagcgcga gaccgttcgt gcgtacggcg gccgcatcgc catcgtcggc gacgcgaagg
     7201 agcactcgac gagcgagctg cttggaagaa tcaaggcgga gggtggcccg ccttcgcgcc
     7261 aggcgccgcg gcgcggcagg cctgagcctt gagccgtgag ccgtgagcct tgagccgtta
     7321 ctatgcgtgt cctcctcgtc cgcctcggcg ccatgggcga catcgtgcac gcgctgccag
     7381 tggcggcggc gttgcggcag cggtttccct tcgcccggat cgactgggtg gtggacgcgc
     7441 ggtacgcggc gctgctcgat ctcgtccccg cgatcgacgg ccggatcgtc gttgccgccc
     7501 gggatcaggc gccggccggc gcgggccgcg cgccgggaag ccccggccgc gacggcgttg
     7561 cggacgggcc caccggcgat cgcgcgcggc ggacgttttc ggcgggcctt ggctggctca
     7621 ggctggtgcg ggcgctgcgg cgcgagcggt acgaggtcgc gctcgacctg caggggctgg
     7681 tgaagtcggc cgtggtcgcg cgggcctcgg gggcccggcg cgtgatcggg ttcggccggc
     7741 cctggctgcg cgagccgctc gccgcgcgct tctacaccga gcgcacggcc gtggcgcgcg
     7801 gcggccacgt ggtggacaag aatctcgcgc tgctgtcggc gctcggcatc ggcaccgcgg
     7861 cgcgggtgtt cccgctggcg gccggtgaca tgaggcccgt ggaggagatg cgccggctcc
     7921 accccgaggc cgcccgcggc ttcgtcctgc tgaaccccgg cgccgcgtgg ccgaacaagc
     7981 gctggccgcc cgagcggttc ggtgcgctcg ccgccatcgt ccgcaggcgg cacggccttc
     8041 cctcggccgt gctctggggg cccggcgagg aggagctggc ggcgctggtg gtggacgagt
     8101 caggcggggc ggccttcgcc gcgccggcga cgcgcctgcc aggactcctc ggcttggcgc
     8161 ggtcggcgcg cctgatcgtc tcgggcgaca ccggcccgct gcacctcgcc gccgcggcgg
     8221 ggactccggc cgtcgccgtg ttcgggccga ccaaccccga gcgcaacggt ccgtggcacc
     8281 cggccgacct caccgtgtcg cgctacgaca cgtgcgattg ccactacagg cggcagtgcc
     8341 gccgcggccg accctgcatc gcggagatcg aggtggacga ggtggcacgg gcggtggacc
     8401 tgcggctggc gacgtagact ccggccttcg accttgactt cgcgacgcga cttcgtgact
     8461 cgatgacttc gtcaacttcg tggacttcgt ggactgcgcg cggcgcccgt cttctggcgc
     8521 gtaccagggt tcctctgggc ttcctcggcg gcgtcgtcgc gctgtggctg gctcacccca
     8581 cgtggcgcag cctggccgcg ggtgctgcgg tagcggccct cggtgaggcg attcgcgtct
     8641 gggcggccgg ccacctcgag aagggcaggg aagtgacccg ctcgggcccc taccgctgga
     8701 cggctcaccc gctctacctg ggttcggcga t
//
LOCUS       DAOPQX010000132         8725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9027_length_8725_cov_4.515110, whole genome shotgun sequence.
ACCESSION   DAOPQX010000132 DAOPQX010000000
VERSION     DAOPQX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9027_length_8725_cov_4.515110"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="PKK95_08630"
     CDS             <1..1959
                     /locus_tag="PKK95_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOC18318.1"
                     /translation="RRLARGPVARLVASGARAGNDWTAEKQALLDEYDRAGLTSVARQ
                     ARPDSGAAASSKNLAIALAAFELAWVDAGAATTALAQALSLAPLELFGTDEQRQQFLP
                     GAEGPARRGAFVLTEPLPYVGVDTGVLSGKLRVGEWRDGEEPILEVEKRGRFITNMAF
                     ADFVTAAVEGADGRVKGTCLVLLQQDDPGVFDRGTPTRKLVHQLASTTDPSFRLRIPA
                     SRIVGGYSVEEGVIVPRYSHREVIEAVFRRTRVTVGLLTAAKLLSAVEPLLRYHRTRF
                     RGGETTTPGTPRYELGLQQREDALLRLVDVWAAGEASASLGFAAARLFDELDADGNGA
                     GARAGDATDAARAEALAAVLSPACKLWNTSHGARMMREAVSLMGGYGITEDCPGFLGH
                     KWMDAQLEATYEGPEAVQRQLLSVTMTDQLFLSQFHQWAVEMRQLADGRPGTGACTVA
                     SAMELWLWTLRHLLTATDADGKRLYQVSRHGVTFPLADALCWLLAVRYQILDVLELEA
                     RKHEAGGAESDGVVSLLLDLCHVQAAAAAGEVGRICAELVHGYQRHPAWNASGCAACY
                     SAADLEALEQLMPGFESTAGGYADVIGHDGSHQAKAGPCVRFPGLDDFTRLRGKLDGC
                     LTGTRLAKDRAASALSKVPVAEGPDYPL"
     gene            2174..2365
                     /locus_tag="PKK95_08635"
     CDS             2174..2365
                     /locus_tag="PKK95_08635"
                     /inference="COORDINATES: protein motif:HMM:NF033683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HOC18319.1"
                     /translation="MAMMIKDDCISCGACEPECPNEAISQGDTIYVIAADKCTECEGH
                     YDSPKCVEVCPVDECIVKA"
     gene            2517..3962
                     /locus_tag="PKK95_08640"
     CDS             2517..3962
                     /locus_tag="PKK95_08640"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA Peptidase M64"
                     /protein_id="HOC18320.1"
                     /translation="MLTTFRLACLVLFGVAAIAVCAAAQPSSPSPFAPFLDKTMRLDF
                     YHTGAGGHEVLSLDRVVSDGLWAGSRTRLIDDTNLGPYLFEVIDRATNRPIYSRGFAS
                     IYGEWETTDEARQGVARTFHESLRFPWPKAPVQVVVKKRDARNAFREVWSTLVDPESR
                     FVTAAEIAPQGRVWTVFENGPAAAKVDLLILGDGYSEAQLPKFHKDVERLVKVLFEHE
                     PFKSRRSDFNVRAIDLPTPKSGVNRPRTGDFRRTPLGVSYNIFDSERYALTLDNRSVR
                     DVAAAAPYDFLEILINEEHYGGGGIFNDHATAAVDTAFAEYIFVHEFGHHFAALGDEY
                     YTSDVAYETGVADRPEPWEPNVTALKDPANLKWKDLVESGTPVPTPWEKAAYEAKSRE
                     VQEKRRQLRAANAPESEMNTLLTSEREWSTAFFGKHAYAGKVGAFEGAAYEAKGLYRP
                     EADCIMFSRDQVGFCRVCQRAISRIIDMYSR"
     gene            complement(4019..4855)
                     /locus_tag="PKK95_08645"
     CDS             complement(4019..4855)
                     /locus_tag="PKK95_08645"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC18321.1"
                     /translation="MVFKRQRTGSVVCPSCGNLVGVNDERCYNCGRWNPGLWGFTPLL
                     RRLGNDLGFTNLVTVTCAALFLAMLLTSGRGLRLVAGPMSFLVPSDVSMIIFGASGAY
                     PVRVLGWWWTILSAGWLHASALHILFNMLWIRQLGPAAGELFGPSRAVIIYVAAGAVG
                     FAVSTIAGSALTLGASAGIMGLLGALVRYGQRTGSSHIGSQAWTWALILFVFGFAMRG
                     VDNWAHGGGFVGGYLAASVLDPLQREKPVHLVLALGSLGLTALSIAASLLLGLSSLLG
                     SR"
     gene            4901..7900
                     /locus_tag="PKK95_08650"
     CDS             4901..7900
                     /locus_tag="PKK95_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC18322.1"
                     /translation="MKTTRAVLVLFVVAWLAPAPPPLSAAQAPPAKAPLAMADILAWK
                     SIGAAVVSDDGKWLAYRVAPVEGDGEVVVRETRGDREWRFGSGDASEGAGELAFSDDS
                     KWLAFAVAPTRTAAAQLKKQRKPLHNSVSLVDLATGKATEFPKIKRFAFSGEQAGWLA
                     LHRYAPEGQAPEKDRPRGSDLILRDLATGAELNVGNVADFAFDKAGRRLAWTIDALDQ
                     AGNGLAVRDLETGAVRPLDSAKASYARPTWTEKGDGLAALRGVDDKTYSEKLYALVAF
                     SGFGDAAPVKVVYDPSADPSFPAGMTISGDRAPEWLEDLSGVLFGIREAKKKADAPAA
                     KDDKAADGDAPAPRGGQDAPKAEGKADSEEKPDLVLWHYQDRRLQSQQQVEEDRDKRF
                     SYLATYRPGEKRFVRLATDEVREVVASPRSKWAIGLDASAYELASSMDGRRYRDIYAI
                     DLATGSRTLAARRVRWFAGPSPDSSRFLYFEDGHYHVHDIASGRFTNLTRALPVSFVD
                     QRDDHNVIKPPTPSLGWSREGDAVLLSDGWDLWRVTVTAGGAAANLTGNGRRDQVRYE
                     RRLVLDRDERGIDFGRPLLVTMHGDWTKKNGIARIDPGGSAATALLWDDAAFARVMKA
                     QKADVYVYSRETADQPPDLYAAGPGLAGGRKLTTLDAQMAKVAWSAGRRLIEFEPAQK
                     LPTARRLQAALYLPANYEAGKRYPTIVYIYERLTQGLNRFAAPTANGFSPSVYTSNGY
                     AVLMPDIAYKLNDPGRSAVWCVLPALEAAVAAGVVDRTKVGLQGHSWGGYQTSFLVTQ
                     TTAFAAAVAGAPLTNMVSMYSLIYKNSGNTNQGIFESSQGRFLGGYWDNWEAYYRNSP
                     VFFARNVKTPLMILHNDKDGAVDFTQGVEYFNTLRRLGKPVWMLEYVGENHGLRKPAN
                     RRDYTVRMREFFDHYLKGAPMAGWMKDGVPRLAMDAHLEERAPLLKGTPAGEPARAGD
                     DAKPAEGEKTGTPKAAPDKKEPGQGPV"
     gene            8017..>8725
                     /locus_tag="PKK95_08655"
     CDS             8017..>8725
                     /locus_tag="PKK95_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC18323.1"
                     /translation="MRKPVVLITGAGGEIGHGLIERLAGDGTRPIITLDLNPLAPGIA
                     KMVHREIQGSILDANLLDRIVAEYEVDLVFHLAALLSTRSEFTPIAAHEVNVGGTLNM
                     LEFAQKQGESHGRPVVFLYPSSIAVYGMPDLETKGRAGEVKEDQYNHPITMYGCNKLY
                     CEQLGHYYSRHYKQLAKEKLSGRVDFRCIRFPGLISAVTVPSGGTSDFAPEMIHAAAR
                     GEAYGCFVRPDTRIPFMA"
BASE COUNT         1420 a         2926 c         3141 g         1238 t
ORIGIN      
        1 cgccggttgg cgcgcgggcc ggtggcgcga ctggtggcct cgggcgcgcg cgccgggaac
       61 gactggacgg ccgaaaagca ggcattgctc gacgagtacg accgggcggg cctcacctcg
      121 gttgcacggc aggcgaggcc cgactcggga gccgcggcca gctcgaagaa cctggccatt
      181 gccctcgccg cgttcgagct ggcgtgggtg gacgctgggg ccgcgaccac tgccctggca
      241 caggcactgt cgctcgcgcc gctcgagctg ttcggcaccg acgagcagcg gcagcaattc
      301 ctgccaggcg cggaggggcc ggcgcggcgc ggcgcgttcg tgctcaccga gccgctgccg
      361 tacgtcggcg ttgacacggg cgtcctgagc ggcaagctcc gggtgggcga gtggcgcgac
      421 ggcgaggagc cgatcctgga ggtggagaag cgcggccgct tcatcaccaa catggcgttc
      481 gccgacttcg tcacggcggc cgtcgaggga gccgacgggc gcgtcaaggg gacgtgcctg
      541 gtgctgctcc agcaggacga ccccggagtg ttcgaccgcg gcacgcccac gcgcaagctc
      601 gttcaccagc tggcgtcgac caccgacccg tcgttccgcc taaggatccc ggccagccgg
      661 atcgtcggcg gctactccgt ggaggagggc gtcatcgttc cacgctactc ccaccgggag
      721 gtcatcgagg ccgtgttccg gcggacgcgc gtgacggtcg gcctgctgac cgcggcgaag
      781 ctgctctcgg cggtggagcc gctgttgcgg taccaccgca cccgcttccg cggcggcgag
      841 acgaccacgc ccggaactcc gcgctacgag ctggggctgc agcagcgcga ggacgcactg
      901 ctgcgcctcg tggacgtgtg ggcggccggc gaggcaagcg cctcgctcgg gttcgcggcg
      961 gcacgattgt tcgacgagct ggacgcggat gggaacgggg ccggggcgcg tgcgggcgat
     1021 gcgaccgacg ccgcccgggc cgaggcgctg gcggcggtcc tgtcgccggc gtgcaagctg
     1081 tggaacacca gccacggggc gcgcatgatg cgcgaggcgg tcagcttgat gggcgggtac
     1141 ggcatcaccg aggattgccc cggcttcctc gggcacaagt ggatggacgc ccagctcgag
     1201 gccacctacg aggggcccga ggcggtccag cggcagctgc tgtcggtgac gatgaccgac
     1261 cagctcttcc tgtcgcagtt ccatcagtgg gccgtggaga tgcggcagct ggccgatggg
     1321 cggcccggga cgggggcgtg caccgtggcc agcgcgatgg aactgtggct gtggacgctg
     1381 cggcacctgc tgaccgccac cgacgccgac ggaaagcggc tgtaccaagt gtcgcggcac
     1441 ggcgtgacgt tcccgctggc cgatgcgctg tgctggctgc tggccgtgcg gtaccagatt
     1501 ctcgacgtgc tcgagctcga ggcgcggaag cacgaggctg gcggtgcgga gtcggacggt
     1561 gtggtgtcgc tgctgctcga tctctgccac gtccaggcgg ccgcggcggc cggagaggtg
     1621 gggcgcatct gcgccgagct ggtgcacggc taccagcgcc atcctgcgtg gaacgcttcg
     1681 gggtgcgccg cgtgttacag cgccgccgac ctcgaggcgc tcgagcagct gatgcccggc
     1741 ttcgagtcca ccgcgggggg ctacgccgac gtgatcggcc acgacgggtc gcaccaggcc
     1801 aaggccgggc cgtgcgtccg ctttccgggg ctggacgact tcacgcggct gaggggcaag
     1861 ctggacgggt gcctcaccgg cacgcgcctg gccaaggacc gggcagcgtc cgccctgtcg
     1921 aaggtgccgg ttgccgaggg tcctgactac cccttatgat ggccggcacg gttcgggcag
     1981 gcgagtgctg tctcgcgccg gatgaccggc agcccgggct gacggctttt cggcagcccc
     2041 gccgggaact tgccgcgaac ggcgcgcggc gcgtaaaatg ggcctgtaat cggtcggttc
     2101 caatcggacc accttttgca cttctcttat tctgccggcc gattttggcc cctgaacagt
     2161 taggagcgga acgatggcga tgatgatcaa ggatgattgc atcagctgcg gtgcttgcga
     2221 gcccgagtgc cccaacgagg caatcagcca gggcgatacg atttacgtga tcgcggccga
     2281 caagtgcacc gagtgcgagg gccactacga ctcaccgaag tgcgtcgagg tctgcccggt
     2341 cgacgagtgc atcgtcaagg cctaggctgc gggatcgacg tacgtcgcgc agcccttcag
     2401 ggctgcggga tcgggctgcg gctcgtccct gcaataatcc aagtcgcaat cacgagctct
     2461 cgctgccggt cggccggtgc gagggctcgt gctttgtact gtgagaaaat cccgccatgc
     2521 tgacgacctt ccgccttgcg tgcctggtgc tctttggcgt ggcggcgatc gcggtgtgcg
     2581 cggccgccca gccctcttct ccctcaccgt tcgcgccgtt cctcgacaag acgatgaggc
     2641 tcgacttcta ccacaccggc gccggcgggc acgaggtcct gagcctcgac cgcgtggtgt
     2701 cggacggcct ctgggccggc agccggacgc ggctcatcga cgacacgaat ctcggcccct
     2761 acctgttcga ggtgatcgat cgggcgacga accgcccgat ctactcgcgc ggcttcgcgt
     2821 cgatctacgg ggagtgggag accaccgacg aggctcggca gggggtggcg cggacctttc
     2881 acgagtcgct gcgctttcca tggccgaagg cgcctgtgca ggtcgtggtg aagaagcgcg
     2941 atgcccggaa cgcctttcgc gaggtctggt cgacgctggt ggatcccgag tcgcggttcg
     3001 tcaccgcagc cgagattgcg ccgcaggggc gggtgtggac ggtgttcgag aacgggccgg
     3061 cggccgccaa ggtcgacctg ctcatcctgg gggacgggta cagcgaggcg cagctgccga
     3121 agttccacaa ggacgtcgag cggctggtca aggtgctgtt cgagcacgag ccgttcaaga
     3181 gccggcggtc cgacttcaac gtgcgggcca tcgacctgcc cacgccgaag agcggcgtga
     3241 accggccgag gaccggcgac ttccggagga cgccgctcgg cgtgtcgtac aacatcttcg
     3301 attcggagcg gtacgcactg acgctcgaca accggtcggt gcgcgacgtg gcggccgcgg
     3361 cgccgtacga cttcctcgag atcctgatca acgaggagca ctacggcggc ggaggcatct
     3421 tcaacgacca cgccacggcc gccgtggaca ccgcgtttgc ggagtacatc ttcgtccacg
     3481 agttcggtca ccacttcgcg gcgctgggtg acgagtacta cacgtccgac gtggcgtacg
     3541 agaccggcgt cgccgatcgg cccgagccgt gggagccgaa cgtcaccgcg ctcaaggacc
     3601 cggccaacct caagtggaag gacctggtgg aatcggggac gccggttccg acgccgtggg
     3661 agaaggcggc ctacgaggcg aagagtcgcg aggtgcagga gaagcggcgg cagctgcgtg
     3721 cggccaacgc gcccgagagc gagatgaaca cgctgttgac ctccgagcgg gagtggtcga
     3781 ccgcgttctt cgggaagcac gcgtacgccg ggaaggtggg agcgttcgag ggggcggcct
     3841 acgaggcgaa ggggttgtac cggcccgagg cggactgcat catgttcagc cgcgaccagg
     3901 tggggttctg ccgggtctgc cagcgggcca tcagccgcat catcgacatg tactctcgct
     3961 gatccctgtt gagaccgacg tgacaaatgg gccaggccct caaggcccgg ccctggcgtc
     4021 agcgggagcc gagcagggaa gacaggccga gcagcagcga ggccgcgatc gacagggccg
     4081 tcagccccag ggaaccgagc gccagcacca ggtgcaccgg cttctcgcgc tgcagcgggt
     4141 cgagcaccga ggccgccagg tagccgccga cgaagccgcc gccgtgcgcc cagttgtcca
     4201 cgccgcgcat ggcgaacccg aagacgaaca ggatcagcgc ccacgtccag gcctggctgc
     4261 cgatgtggct gctgccggtc cgctgaccgt agcgcaccag ggcgcccagc aggcccatga
     4321 tgcccgccga cgcccccagc gtcagcgccg agccggcaat cgtgctcacg gcgaagccga
     4381 cggccccggc cgccacgtag atgatcaccg cgcggctcgg cccgaacagc tccccggctg
     4441 cggggcccag ctggcggatc cacaacatgt tgaagaggat gtgcagggcg ctggcgtgca
     4501 gccagccggc gctgaggatc gtccaccacc agcccagcac gcgcacggga tacgccccgc
     4561 tcgccccgaa gatgatcatc gagacgtcgc tcggcacaag gaaggacatc ggcccggcca
     4621 ccaggcgcag cccgcggccc gaggtgagca gcatcgcgag gaagagcgcg gcgcaggtca
     4681 ccgtcaccag gttggtgaac ccaaggtcgt tcccaagccg gcgaagcagc ggcgtgaacc
     4741 cccagagccc cgggttccac cggccgcagt tgtagcagcg ttcgtcgttg acgccgacaa
     4801 ggttgccgca ggagggacag acgaccgagc cggtgcgttg gcgcttgaac accatgtaat
     4861 catagcagcg ccccgcgttg gcccctccag ggagaactcg atgaagacca cgcgtgccgt
     4921 cctcgttctg ttcgtcgtcg cctggctcgc tccggcgccg cctccgctga gcgccgcgca
     4981 ggcgcccccg gccaaggcgc cgctcgccat ggccgacatc ctcgcctgga agagcatcgg
     5041 cgccgctgtc gtctccgacg acgggaagtg gctggcgtac cgggtggctc ctgtggaggg
     5101 cgacggcgag gtggtggtgc gcgagacccg gggcgaccgc gagtggcgct tcggcagcgg
     5161 cgacgcgagc gaaggtgccg gcgagctggc gttttccgac gactcgaaat ggctcgcctt
     5221 cgccgtggcg cccacgcgca cggcggctgc gcagctgaag aagcagcgca agccgctcca
     5281 caacagcgtc tcactcgtgg acctcgccac cgggaaggca acggagttcc cgaagatcaa
     5341 gcgcttcgcg ttctcgggcg agcaagcggg ctggctggcc ctccaccgct acgcccccga
     5401 gggccaggcg cctgagaagg accggccgcg cggctccgac ctgatcctcc gcgacctggc
     5461 cacgggagcc gagctgaatg tcggcaacgt ggccgacttc gccttcgaca aggcgggccg
     5521 ccgccttgcc tggacgatcg acgcgctcga tcaggcgggc aacggcctcg cggtgcgcga
     5581 cctcgagacg ggggcggtgc ggccgctcga cagcgcgaag gcttcgtacg cgcgcccgac
     5641 ctggacggag aagggggacg gcctggcggc cctccggggc gtcgatgaca agacctactc
     5701 cgagaagctg tacgcgctgg tggccttcag cgggttcggc gacgcggctc cggtcaaggt
     5761 cgtgtacgac ccgtcggccg acccgtcgtt cccggcgggc atgacgatca gcggcgaccg
     5821 ggcccccgag tggctggaag acctctcggg cgtgctgttc gggatccgcg aggcgaagaa
     5881 gaaggccgac gcgccggccg ccaaggacga caaggcagcc gacggcgacg cgcccgcgcc
     5941 gcggggcggg caggacgccc cgaaggccga aggcaaggcc gacagcgagg agaagccgga
     6001 cctcgtcctg tggcactacc aggacaggcg gctgcagtcg cagcagcagg tcgaggagga
     6061 ccgcgacaag cggttctcgt acctggccac ctaccgcccc ggcgagaagc ggttcgtccg
     6121 cctggccacc gacgaggttc gagaggtcgt cgcctcgccg cgctcgaagt gggcgatcgg
     6181 tctcgacgcg tcggcgtacg agctggcgag cagcatggac ggccggcggt accgcgacat
     6241 ctacgcgatc gatctggcca ccggctcgcg cacgctggcg gccaggcgcg ttcgctggtt
     6301 cgcggggccg tcgcccgact cgagccgctt cctctacttc gaggacggtc actaccacgt
     6361 gcacgacatc gcctccggcc gtttcaccaa cctgacgcgc gcgctgcccg tgtcgttcgt
     6421 ggatcagcgc gacgaccaca acgtgatcaa gcccccgacg ccgtcgctcg ggtggtcgcg
     6481 ggaaggggat gcggtgctgc tgtcggacgg ctgggacctg tggcgcgtca cggtcacggc
     6541 aggcggcgcc gcggcgaacc tgacgggaaa cggccgccgc gaccaggtcc gctacgagcg
     6601 ccgcctggtg ctggaccgcg acgagcgggg cattgacttc ggccgcccgc tgctggtgac
     6661 catgcacggc gactggacga agaagaacgg catcgcccgc atcgacccgg gcgggtcggc
     6721 agcgacggcg ctgctctggg acgacgccgc gttcgccagg gtgatgaagg cgcagaaggc
     6781 cgacgtgtac gtgtactcgc gggaaaccgc cgaccagccg cccgaccttt atgccgccgg
     6841 tcccggcctg gcggggggcc ggaagctgac caccctcgac gcacagatgg cgaaggtggc
     6901 ctggtcggcc gggcgccgcc tgatcgagtt cgagccggcg cagaagctgc cgacggcccg
     6961 tcggctgcaa gcggcactgt acctgccggc caactacgag gccggcaagc gctacccgac
     7021 gatcgtctac atctacgagc ggctcacgca gggcctgaac cgcttcgcgg cgcccaccgc
     7081 gaacggcttc agcccttccg tgtacacgag caacggctac gcggtgctga tgccggacat
     7141 cgcctacaag ctgaacgacc cggggcggtc ggccgtctgg tgcgtcctgc ccgccctcga
     7201 ggctgccgtg gccgccggag tcgtcgaccg gaccaaggtg ggtctgcagg ggcactcgtg
     7261 gggcggctat cagacctcgt tcctggtaac gcagaccacc gcgttcgcgg ccgcggtcgc
     7321 cggcgccccc ctgacgaaca tggtcagcat gtactcgctg atctacaaga actcggggaa
     7381 caccaaccag ggaatcttcg agagcagcca ggggcgcttc ctcggcgggt actgggacaa
     7441 ctgggaggcg tactaccgca actcgccggt cttcttcgcg cgcaacgtca agacgccgct
     7501 catgatcctc cacaacgaca aggacggggc ggtcgacttc acccagggcg tcgagtactt
     7561 caacacgctg cgccggctgg gcaagccggt ctggatgctc gagtacgtcg gcgagaacca
     7621 cggcctgcgc aagccggcga acaggcgtga ctacacggtg cggatgcgcg agttcttcga
     7681 tcactatctc aagggcgcgc cgatggccgg gtggatgaag gacggcgtgc cgcgcctggc
     7741 gatggacgcg cacctggagg agcgggcgcc gctgttgaag gggacgccgg ccggagagcc
     7801 cgcgcgagct ggcgacgacg ccaagccggc cgagggggag aagacgggga ccccgaaggc
     7861 ggccccggac aagaaggaac cggggcaagg gccggtgtaa aatcgtctgg tcccacgcac
     7921 gggccggcgg ccgccggccc gtgccaccgg cacgaggttc acccccacat cacaaatgcg
     7981 tcgcaagctt gcgagaaaga cgagggaggt ctgagcgtgc ggaagcctgt tgttttgatc
     8041 acgggtgccg gcggcgagat cggccacggc ctgatcgagc gcctcgccgg cgacggaacc
     8101 cgtcccatca tcaccctcga cctcaacccc ctcgcgccgg gcatcgcgaa gatggtgcac
     8161 cgggagatcc aaggctcgat cctcgacgcc aacctcctcg accgcatcgt ggctgagtac
     8221 gaggtggacc tcgtcttcca tttggccgcg ctcctctcga cacgctctga gttcacgccg
     8281 atcgcggcgc acgaggtgaa cgtcggcggc accttgaaca tgctcgagtt cgcccagaag
     8341 cagggcgagt cgcacgggcg gccggtcgtg ttcctgtacc cgtcgtcgat cgcggtgtac
     8401 gggatgcccg atctcgagac caagggacgg gccggcgagg tcaaggagga tcagtacaac
     8461 cacccgatca cgatgtacgg gtgcaacaag ctgtactgcg agcagctggg ccactactac
     8521 agccgccact acaagcagct ggcgaaggag aagctgagcg gccgggtgga cttccggtgc
     8581 atccggttcc ctggcctgat ctcggcggtg acggtcccct cggggggtac ctcggacttc
     8641 gcccccgaga tgattcacgc ggcggcccgg ggcgaggcgt acggctgctt cgtgcgcccc
     8701 gacacgcgca tccccttcat ggcga
//
LOCUS       DAOPQX010000133         8724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9029_length_8724_cov_4.417118, whole genome shotgun sequence.
ACCESSION   DAOPQX010000133 DAOPQX010000000
VERSION     DAOPQX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9029_length_8724_cov_4.417118"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKK95_08660"
     CDS             <1..760
                     /locus_tag="PKK95_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOC18324.1"
                     /translation="AGTTEGITALQMDIKVAGVTSAVMTEALEQARRGRLHILGIMEQ
                     TLQAPRTNISMFAPRIVTIQIPVDKIRDVIGPGGKVIRSIIERTGVKIDVEDDGRVNV
                     ASADGDSAQRAIAIIQELTATPELNKSYLGKVQRITDFGAFVEIMPGVDGLLHVSEIA
                     KHRVKDVRDELKEGEQLMVKVINIDPTGKIRLSRKALLEEAEPGAAAQAPAHAGHAPS
                     HEGHGPSHEGHAAAGRRPERAEHGDRPRGPRRRH"
     gene            852..1256
                     /locus_tag="PKK95_08665"
     CDS             852..1256
                     /locus_tag="PKK95_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOC18325.1"
                     /translation="MCSRRWTVAALVVFVALAGMAGWRVAAAKAAPAPALEQSKGPVN
                     VHARKYSYTPSRIEVEEGDLVAIRFEADDIPHSFTIDDDAYRISKRAAPGHPVVFEFR
                     AEKVGNFPYYCNLAADPECKKMRGELVVRRRR"
     gene            1284..2420
                     /locus_tag="PKK95_08670"
     CDS             1284..2420
                     /locus_tag="PKK95_08670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOC18326.1"
                     /translation="MNEDRAARYHQRRRRAAVAGVAWSATLLLAMVLTPASVALRDIA
                     ERGASSLPVPPWALPSATVALYAALLGLLHETGALAIAAYGWFLDRRYDLALDRPGGW
                     MLDQVKGLAVAAALALPAFCGLYLAIERWPSGWWLPAAAAFALLVTVMARLAPVVLLP
                     LFFQFHPLERQDLRQRLMDLCRRAGIEAIDACVWQVSDRTRRANAALAGLGRTRRILI
                     SDTLLANARDEEVEAVLAHEIAHHVRADIWRGIALEAAVALAGFYLASVVLEAAWRPF
                     GLRGVADVAGLPVLLLAAGVLSALLLPLANAYSRHVERTADRFAIRLTGNPEAFASAM
                     QRLGVLNLAEIEPSRLALWLFHSHPPVASRIAAAKKAGRDAGPD"
     gene            complement(2429..3232)
                     /locus_tag="PKK95_08675"
     CDS             complement(2429..3232)
                     /locus_tag="PKK95_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOC18327.1"
                     /translation="MQAKRISAPGSLLSLMVVAAAVALAGCDVVVSGLNAKGRAEQPW
                     SRNYTLSPTGTVEVVNGNGKIAVRGGEGNQVEVKAQVVARAATDEQAQEYLKQIEIRE
                     VVSGDHIRLETTSPRLTGSRAEVQYEIVVPQTASLRLTNTNGQIQVTAVSGEVRAETT
                     NGGVKGTELSGRVEASTTNGGVDLDLIEVAEGGVRAETTNGGVSLRMPASVKANLRAS
                     CVNGGISVTGFQLEGGESTRRRVEGRLNGGGPTVEAETTNGGVRVAARQ"
     gene            complement(3312..7034)
                     /gene="smc"
                     /locus_tag="PKK95_08680"
     CDS             complement(3312..7034)
                     /gene="smc"
                     /locus_tag="PKK95_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOC18328.1"
                     /translation="MRLQKLQISGFKSFSDRSELSFDRGVTAIVGPNGCGKSNVADAI
                     VWVLGEQSAKSLRGDQMMDVIFNGSEARKPTAAAEVRLMLSEVTAANRLLAEDEGDRE
                     ALARNVEVTRRLYRSGDSEYLINGEQCRLKDVHELLMDTGLGAKAYAIIEQGKIGQIL
                     SSRPTDRRQLIEEAAGVTKYKARRRAAELKLEASQQNLTRIDDIIFEVEKQRAALKRQ
                     AAKARRYKRLREELRRWEKVQFARKYRVLAEAIETARARLAGRRAEESAAAARLAASE
                     DALSRIRLELAQAEADAARQREAAHARELEIDRRQNQISLNQQQLEHLVARRADVAEE
                     LVAIASRHDPARQALEERREAAARAAVERDEAAEVLTRANAEHAEAARAAHACEAEVD
                     ERRQALFLLQSSVASLQHAVENARTSRERVVDGLSRLQAEQADVRTEETRLAEERASV
                     RARLAEAESAIEQLQQQADARRQELAAAREDLERERRETRTREQELATVEARLASLEE
                     LEAARAQYGEAARLVLSGSVSIPHRGSLADCLEVERGYERAVEGVLGDRLQYVLVDDH
                     AAAERGLAVVREHRAGRCGFLVAGEAGGGAVEQEVAPPVEGLVPLTSIARVASGPRDA
                     VAGAIGRAWVAPSFAAAAAASAQTTDPIATREGDVFIGPHLVSGGLREEARGILSTKR
                     EIKELRDRAGQVRSQLEALNERTGAGEAAIETLTAAIALLEADGHRQEKVIVGLEAQA
                     AKVEENAERLSRREDLLATEMARAEEQRRALDEREREASVEIERRLAERLTADSLLAE
                     GQRQLQGAREAAESVAGRVADARAQHATLVERAAALVGEVARLEEALAELASREAARR
                     QEAEQNEARQAELRLAIEEARRRLDEDGAELERLRETVRQADEALAERHAESDRQEAE
                     IRAARSALDDLRAALTELDVARATAEGDLAHLSAACLESVQLPLDEVVAEVEQAEREG
                     RAAPPSPEPEAVEADEAEEEGQTPEATEGQPFAAAGELTLQVEAAGPTVPSRAEPETP
                     EEAIAALKERIEKLGPVNMMAIEQFDELEGRHTFLTTQRQDLRESIAATGEAIRRIDE
                     TSKQRFREAFVVINENFQGTFATLFGGGRAGLALVNEADVLESGIEIVAQPPGKRLQN
                     VQLLSGGEKALTAIALMFAIFRYKPSPFCVLDEIDAPLDDANIGRFVELLKQMQEQTQ
                     FIIITHSRKTMEIADRLYGVTMEEPGVSKLISVRLN"
     gene            complement(7116..>8724)
                     /locus_tag="PKK95_08685"
     CDS             complement(7116..>8724)
                     /locus_tag="PKK95_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOC18329.1"
                     /translation="AVVVSALVGGLLGRSAMATDTRQQDYRTFTEALAAVEANYIDKV
                     DTARLVYGAVEGMLQTLDPHSSFMDPDLYRRMRERQEGRYYGLGITIVPVNGDITVVS
                     LFEGSPAYRKGIRRGDIIARIEGEDAKGWSSDDAVKRLRGPKGTTVQISLKRPGYDQL
                     IEVEVPRDEINIPSIPAYFMADAETGYVRLQDFAENTDRDLDAALKDLSGQGMKRLLL
                     DLRQNPGGPLDQAIRVTNNFLPRGSLIVSTRGRAHNSDKDYVATEAGEYPSMPIVVLV
                     NRNSASASEIVSGALQDHDRALIVGETTFGKALVQSVYTIAEQAGLALTTARYYTPSK
                     RMIQRPWDGTFDEYLNYTLKRQEPNRPHKPSELRYTAGGRKVYSGGGIEPDRRLDGPI
                     EGFDPTPFGRLLYNRQLFATFAERFAAEGDERIAPRPDQRRVSKGFVVDDRMLDDFKA
                     FVREQKVKVDEAAFAADEAFLRAMIHYDIDLALFGVSEARRNLVATDPQTRFALQLFS
                     EAAQLAELRKAKPASSRGGMTGGFPNN"
BASE COUNT         1165 a         3157 c         2990 g         1412 t
ORIGIN      
        1 ggccggcacg accgagggga tcactgccct ccagatggac atcaaggtgg ccggggtgac
       61 ctcggcggtg atgaccgagg cgctcgagca ggcgcgccgc ggccgcctgc acatcctcgg
      121 catcatggag cagacgctgc aggcgccgcg caccaacatc tcgatgttcg cgccccgcat
      181 cgtcaccatc cagatcccgg tggacaagat ccgcgacgtc atcgggccgg gcggcaaggt
      241 gatccgcagc atcatcgagc gcaccggggt gaagatcgac gtcgaggacg acggccgggt
      301 gaacgtcgcg tcggccgatg gcgactcggc ccagcgcgcg atcgcgatca tccaggagtt
      361 gacggcgacg cccgagctga acaagtcgta cctcgggaag gttcagcgca tcaccgactt
      421 cggcgcgttc gtcgagatca tgccgggcgt ggacggcctg ctgcacgtct cggagatcgc
      481 gaagcaccgc gtcaaggacg tgcgcgacga gctgaaggag ggcgagcagt tgatggtgaa
      541 ggtcatcaac atcgacccga ccggcaagat caggctcagt cgcaaggcgc tgctcgagga
      601 ggccgagcct ggcgccgcgg cgcaggcccc ggcgcacgcc ggccacgcgc cttcccacga
      661 gggccacgga ccttcgcacg agggacacgc cgcggcgggc cggcgccccg aacgcgccga
      721 gcacggcgac cgcccgcgag ggccacgccg ccggcattag cccggagggg ttgacgtatt
      781 cgctccggct cgagggtggg ctgaagctct gccctcgagc cgccgataaa ggagatcgga
      841 aggggagagg tatgtgctca cgccgctgga cggtggccgc actcgtcgtc ttcgtcgccc
      901 tcgccggcat ggccgggtgg cgagtcgcgg ccgcgaaggc ggctcctgcc ccggccctgg
      961 agcagagcaa gggtccggtc aacgtgcacg cccggaagta ctcctacacg ccctcccgga
     1021 tcgaggtcga agagggtgac ctcgtcgcca tccgtttcga ggccgacgac atcccgcaca
     1081 gcttcaccat cgacgacgac gcctaccgga tctcgaaacg ggcggcaccc ggccaccccg
     1141 tcgtgttcga gttccgtgcc gagaaggtgg gcaacttccc gtactactgc aacctggcgg
     1201 cagaccccga gtgcaagaag atgcgcggcg agctggtcgt tcgcaggcgg cgctgacgct
     1261 gcgctcgagg tagcggagcc ggcgtgaacg aggacagggc ggcccgctat caccagcgca
     1321 ggcgcagggc cgccgtggcg ggggtggctt ggagcgccac gctgctgctg gccatggtcc
     1381 tgacgccagc ctccgtcgcc ctgcgcgaca tcgcggagcg gggcgcgtcc tcgctgccgg
     1441 ttcccccgtg ggcgctccca tcggcgaccg tcgccctcta cgccgcgcta cttggccttc
     1501 tccacgaaac gggcgccctg gcgattgccg cctacgggtg gttcctcgac cggcgctacg
     1561 accttgccct ggaccgcccg ggcggctgga tgctcgacca ggtcaagggg cttgccgttg
     1621 ccgcggccct tgccctgccg gccttctgcg ggctgtacct cgccatcgaa cggtggccgt
     1681 cgggctggtg gctgccggca gcggccgcct tcgcgctgct cgtcaccgtg atggcccgtc
     1741 tcgccccggt cgtcctgctg ccgctcttct ttcaattcca cccgctcgag cgccaggatc
     1801 ttcgacagcg gctcatggac ctgtgccgcc gtgccggcat cgaggccatc gacgcctgcg
     1861 tgtggcaggt gagcgaccgg acccgacgcg cgaacgccgc gctcgcgggg ctggggcgca
     1921 cgcgccggat cctcatttcg gacaccctgc tcgccaatgc ccgggacgag gaggtggagg
     1981 cggtgctggc gcacgagatc gcgcatcacg tgcgggcgga catctggaga ggcatcgcgc
     2041 tcgaggcggc cgtggccctg gccggcttct acctggcctc ggtcgtcctg gaggcggcct
     2101 ggcgcccgtt cgggctgcgg ggagtggccg acgtggcagg cctgccagtg cttctactcg
     2161 cggcgggagt cttgtcggcc ctgctgctgc cgctcgccaa cgcctattcg cggcacgtgg
     2221 agcggacggc agaccgcttc gccatccgcc tgaccggaaa ccccgaggcg ttcgcatcag
     2281 cgatgcagcg cctcggggtg ttgaacctgg ccgagatcga accgtcccgc ctggcgctgt
     2341 ggctcttcca cagccacccg cccgtggcct cgcggatcgc cgcggccaaa aaggcaggcc
     2401 gggacgccgg ccccgactga cacacgccct actggcgggc cgcgacccgg acgccgccgt
     2461 tggtcgtctc ggcctcgacg gtcgggccgc cgccgttcag ccgcccctca acccgcctgc
     2521 gggtcgattc gccgccctcg agctggaagc cggtcaccga gatcccgccg ttcacgcagc
     2581 tcgccctgag gttggccttg acactggccg gcatgcggag tgacacgccg ccgttggtcg
     2641 tctcggcgcg cacgccgccc tccgccacct cgatcaggtc gaggtcgacg ccgccgttgg
     2701 tcgtggacgc ctcgacgcgc cccgagagct cagtgccctt gacgccgcca ttggtcgtct
     2761 cggcgcgaac ctcgcccgac acggcggtga cctgtatctg cccgttggtg ttggtcaggc
     2821 ggagtgaagc cgtctgcggc acgacgatct cgtactggac ttccgcccgc gagccagtca
     2881 ggcgcgggga cgtcgtctcg aggcggatgt ggtcgccgct gacgacctcg cgaatctcga
     2941 tctgcttcag gtactcctgc gcctgctcgt cggtggcggc ccgggcgacg acctgagcct
     3001 tcacctcgac ctggttcccc tctccccccc gcacggcaat cttgccgttg ccgttcacga
     3061 cctcgacggt cccggtcgga gagagcgtgt agttccggct ccagggctgc tcggccctcc
     3121 ccttggcgtt caggcccgac accaccacat cgcacccggc caaggcgacg gcggcggcga
     3181 cgaccatcag cgagagaaga gaacccggcg cggagatgcg cttcgcttgc atgaatccag
     3241 cctcctgcct gagatatacg gtgggcgggc aggctatgtt ctgaaaggat ggaaaatggc
     3301 gcccctgact cctagttgag ccgcacggag atcagcttcg aaacgcctgg ctcctccatc
     3361 gtcaccccgt agagccggtc ggcaatctcc atggtcttcc ggctgtgggt gatgatgatg
     3421 aactgcgtct gctcctgcat ctgcttgagc agctcgacga accggccgat gttggcgtcg
     3481 tcgagcgggg cgtcgatctc gtccagcacg cagaacgggc tgggcttgta gcggaagatc
     3541 gcgaacatca gggcgatcgc cgtcagcgcc ttttcgccgc ccgacagcag ctgcacattt
     3601 tggaggcgct tgccgggagg ctgggccacg atctcgatcc cgctctccag cacgtcggcc
     3661 tcgttcacca gcgccagccc ggcgcggccg cccccgaaca gcgtcgcgaa ggttccctgg
     3721 aagttctcgt tgatgacgac gaacgcctcg cggaagcgct gcttgctggt ctcgtcgatc
     3781 cggcgaatgg cctcgcctgt cgcggcaatc gactcgcgca ggtcctgccg ctgggtcgtg
     3841 aggaaggtgt ggcggccctc gagctcgtcg aactgctcga ttgccatcat gttcaccggc
     3901 cccagctttt cgattcgctc cttgagcgcc gcgatcgcct cctcgggcgt ctcgggctcg
     3961 gcgcggctcg gcacggtcgg gccggcggcc tcgacctgga gggtcagctc ccctgccgcc
     4021 gcgaacggct gcccttccgt tgcctccggc gtttgccctt cctcctcggc ttcgtcggcc
     4081 tcgacggcct ccggctcggg tgaggggggc gccgcccggc cctcgcgctc ggcctgctcg
     4141 acctcggcca ccacctcgtc caggggaagc tggaccgatt caaggcaggc cgcggaaagg
     4201 tgcgccaggt cgccctcggc agtcgcgcgg gcgacatcca gctcggtcag cgcggcacgg
     4261 aggtcgtcga gggcggaccg cgccgcccgg atctccgctt cctgcctgtc ggactccgcg
     4321 tgacgctcgg ccagcgcctc gtcggcctgc cgcacggtct cgcgcaaccg ctcgagctcg
     4381 gccccgtcct cgtcgaggcg tcggcgtgct tcctcgatgg ccaggcgcag ttcggcctgc
     4441 ctggcctcgt tctgctcggc ttcctggcgt cgcgccgcct ctcgggaggc cagttcggca
     4501 agcgcctctt cgagcctcgc cacctcgccc accagcgcgg ccgcgcgctc gaccagcgtg
     4561 gcgtgctgag cacgcgcgtc ggccacccgc ccggcaaccg actcggccgc ctcgcgcgcg
     4621 ccctgcagct ggcgctgccc ctcggcgagg agggagtcgg cggtcagccg ctcggccagc
     4681 ctgcgctcga tctcgaccga ggcctcgcgc tcgcgctcgt cgagggcccg gcgctgctct
     4741 tctgcgcggg ccatctcagt ggccagaagg tcttcacgcc tcgagagccg ctccgcgttc
     4801 tcctcgacct tcgccgcctg ggcctcgagg cccacgatga ccttctcctg ccggtgccca
     4861 tcggcctcga gcagcgcgat tgctgccgtc aaggtctcga tggccgcctc cccggcaccc
     4921 gtcctctcgt tgagggcctc gagctgcgag cgaacctgcc cggcacggtc gcgaagctcc
     4981 ttgatctccc gcttggtcga aaggatgccg cgagcctctt cgcgcaggcc gccggacacg
     5041 aggtgcggcc cgatgaagac atccccctcg cgggtggcga tcggatcggt ggtctgggcc
     5101 gacgcggccg ctgccgcggc aaaggagggc gccacccagg cacgcccgat cgccccggca
     5161 actgcgtcac gggggcccga ggcgacccgc gcaatcgagg tcagcggcac cagtccctcg
     5221 accggcggcg ccacctcctg ctccaccgct ccgccaccag cctcgccggc caccaggaac
     5281 ccgcagcgcc ctgcgcggtg ctcgcgcacg accgccagcc cccgttcggc ggcggcatgg
     5341 tcgtccacca ggacgtactg gagccggtcg cccagcactc cctcgaccgc gcgctcgtat
     5401 ccccgctcga cctcgaggca gtcggcgagc gagccgcgat gcggtatcga cacgctgccc
     5461 gagaggacca gccgcgccgc ctcgccgtac tgtgcccgcg cggcctcgag ctcctcgagc
     5521 gaggcgagcc gggcctcgac ggtggccaac tcctgctccc gggtgcgcgt ctcgcggcgt
     5581 tcgcgctcga ggtcttcgcg cgcggccgcc aattcctgcc ggcgcgcgtc ggcctgctgc
     5641 tgcagctgct cgatcgcgct ctcggcctcg gcgagcctcg cacggaccga ggcccgttcc
     5701 tcggccagcc gcgtctcctc ggtccgcacg tcggcctgtt cggcctggag gcgcgacagg
     5761 ccgtcgacga cccgctcgcg cgacgtccgg gcgttctcga ccgcgtgctg gagcgacgca
     5821 accgacgatt gcaggaggaa cagcgcctgg cggcgctcgt ccacctccgc ctcgcaggcg
     5881 tgcgccgcgc gggccgcctc ggcgtgctcg gcgttggcgc gggtcagaac ctcggcagcc
     5941 tcgtcacgct cgacggcggc gcgcgcggcg gcctcccgcc gctcctcgag ggcctggcgg
     6001 gccgggtcgt gccgggacgc gatggcgacc agctcctcgg cgacgtcggc cctgcgggcc
     6061 accaggtgct cgagttgctg ctggttcaga ctgatctggt tctggcgccg gtcgatctcc
     6121 agctcgcgcg cgtgcgcggc ctcgcgctgc cgggcggcat ccgcctcggc ctgggccagc
     6181 tcgaggcgta tgcgcgacag ggcgtcctcg gaggcggcca ggcgcgcggc ggcggccgac
     6241 tcttcggcac gcctgccggc caggcgcgcg cgggccgtct cgatcgcctc tgccagcacc
     6301 cggtacttcc gtgcgaactg cactttctcc cagcgccgga gctcttcccg gaggcgcttg
     6361 tagcgccgcg ccttcgccgc ctggcgcttg agcgccgccc gctgcttctc gacctcgaag
     6421 atgatgtcgt cgatccgggt gaggttctgc tgcgacgcct cgagcttcag ctcggccgcc
     6481 cggcgccgcg ccttgtactt ggtgacgccg gcagcctcct cgatcagctg ccgccggtcg
     6541 gtgggacgcg agctgaggat ctggccgatc ttcccctgct cgatgatggc gtaggccttg
     6601 gcgccgagcc ccgtgtccat caacagctcg tgcacgtcct tgagccggca ctgctcgccg
     6661 ttgatcaggt actcgctgtc gccggaccga tagagtcgcc tcgtgacctc gacgttgcgc
     6721 gccagggcct cccggtcccc ctcgtcttcg gccagcagcc ggttcgcggc ggtgacctcg
     6781 gacagcatca gcctcacttc agccgccgcg gtcggcttgc gggcctcgct gccgttgaaa
     6841 atcacgtcca tcatctggtc gccgcgaagg ctcttcgcgc tctgctcgcc cagcacccag
     6901 acgatggcat cggcgacgtt gctcttcccg cagccgtttg gcccgacgat ggccgtgacg
     6961 ccgcggtcga acgagagctc cgaccgatcc gaaaacgact tgaacccgga aatctgcagc
     7021 ttctgaagac gcatctggac ccctgcccgg cgggacgccg ggccgcggtt cagtctagca
     7081 acgacgaggt attgtggcaa gcacccgaaa gggcactagt tgttggggaa accgcccgtc
     7141 atcccgcccc gggaagatgc cggcttggcc tttcggagct cggccagctg cgcggcctcg
     7201 gaaaagagct gcagggcgaa ccgggtctgg ggatcggtgg cgaccaggtt gcgccgtgcc
     7261 tcggagacgc cgaacagcgc caggtcgatg tcgtagtgga tcatcgcccg caggaacgcc
     7321 tcgtccgccg cgaaggcggc ctcgtcgacc ttcaccttct gctcacggac gaacgccttg
     7381 aagtcgtcga gcatgcggtc gtcgacgacg aagcccttcg agacgcgccg ctggtcgggc
     7441 cggggtgcga tccgctcgtc gccttccgca gcgaagcgct cggcgaaagt cgcgaacagt
     7501 tggcggttgt agagcaggcg cccgaacggc gtggggtcga agccctcgat ggggccgtcc
     7561 aggcggcggt cgggctcgat gccgccgccg ctgtaaacct tccggccgcc ggccgtgtag
     7621 cggagttcgg acggcttgtg cggccggttc ggctcctgcc gcttcagcgt gtagttcagg
     7681 tactcgtcga aggtcccatc ccaggggcgc tggatcatcc gcttgctcgg ggtgtagtag
     7741 cgcgctgtcg tcagggccag ccccgcctgc tcggcgatgg tgtagaccga ctggacgagc
     7801 gccttcccga aggtggtctc accgacgatg agggcccgat cgtggtcctg cagcgcgccg
     7861 ctgacgatct cggaggcgct cgcgctgttg cggttcacca gcacgacgat cggcatcgag
     7921 gggtactcgc cggcctcggt cgccacgtag tccttgtcgg agttgtgggc gcggccgcgg
     7981 gtcgagacga tcagcgaccc ccgcgggagg aagttgttgg tgacccggat cgcctggtcg
     8041 agcggcccgc cggggttctg ccgcaggtcg aggaggagcc gcttcatccc ctggccggag
     8101 aggtccttca gggcggcgtc gaggtcgcgg tcggtgttct ccgcaaagtc ctgcaggcgg
     8161 acgtagccgg tttcggcatc cgccatgaag taggccggga tcgatggaat gttgatctcg
     8221 tcgcggggaa cctcgacctc gatcagctgg tcgtagcccg ggcgcttgag cgagatctgg
     8281 acggtggtgc ccttcggccc gcgcagccgc ttcacggcgt cgtcgctgct ccagcccttg
     8341 gcatcctcgc cctcgatgcg cgcgatgatg tcgccgcggc ggatgccctt ccggtacgcg
     8401 ggcgacccct cgaacagcga cacgaccgtg atgtcgccgt tgacggggac gatcgtgatg
     8461 ccgaggccgt agtagcggcc ctcctggcgc tcgcgcatcc ggcggtacag gtcggggtcc
     8521 atgaagctcg aatggggatc gagcgtctgc agcatgccct cgacggcgcc gtaaaccagg
     8581 cgcgccgtgt ccaccttgtc gatgtagttg gcttcgacgg cggcaagcgc ctcggtaaac
     8641 gtgcggtagt cctgctgccg cgtgtcggtg gccatggcgc tgcggcccag gaggccgccc
     8701 accagggccg aaacgaccac ggcg
//
LOCUS       DAOPQX010000134         8691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9068_length_8691_cov_4.468504, whole genome shotgun sequence.
ACCESSION   DAOPQX010000134 DAOPQX010000000
VERSION     DAOPQX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9068_length_8691_cov_4.468504"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="recA"
                     /locus_tag="PKK95_08690"
     CDS             complement(<1..645)
                     /gene="recA"
                     /locus_tag="PKK95_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOC18330.1"
                     /translation="MPADDRNERTKALDVAVSQIEKQFGKGSIMRLGSKGAITPVDAI
                     PTGSISLDFALGIGGVPRGRVVEIYGPESSGKTTLALQVIAQAQKLGGMAAFVDAEHA
                     LDATYAKKLGVDLENLLVSQPDNGEQALEIVEVLVRSGGVDVVVVDSVAALVPRAEIE
                     GEMGEAQMGLQARLMSQALRKLTGVVSKSKTCLVFINQLREKIGVMFGSPETTTG"
     gene            complement(839..1447)
                     /locus_tag="PKK95_08695"
     CDS             complement(839..1447)
                     /locus_tag="PKK95_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOC18331.1"
                     /translation="MPSPIGHALGALAAGWAVAAPEPKAGTSGRAAGAWRPALRRAGL
                     ILGVALLPDLDLLVGVHSQWTHSIGAVVLVFFAAWLAGRGRHPRLAATLALAYATHPF
                     LDWLGEDGTPPYGVMAGWPFTDRYFHSGADLFTGISRRYWLPDFLAHNLAAVAREVIL
                     LGPFALAGWWWQRLRTRHAAERRFLQPGAASADRGESRGNCK"
     gene            complement(1447..1746)
                     /locus_tag="PKK95_08700"
     CDS             complement(1447..1746)
                     /locus_tag="PKK95_08700"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOC18332.1"
                     /translation="MEPLHSVLPRAIRQLLRAGPMDQEKLAFAWTAAVGPAISRATAV
                     RWCDGRIVEVTASDEAWAREISRDLPEIARRLEALVGAGVVAEVRLASPPRRGRA"
     gene            1818..2399
                     /locus_tag="PKK95_08705"
     CDS             1818..2399
                     /locus_tag="PKK95_08705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOC18333.1"
                     /translation="MRLILASSSPRRSELLTAARIPFEVRPVEVDERPLDGEDPAAYV
                     VRAARDKAGAFGAVPPDTVVLSADTTVVVEDEILGKPADAADAARMLRLLSGRWHEVL
                     SGVVLRHASGETTEVDVSRVRFLPLSEDEVRWYIGTGEPFGKAGAYAIQGLASRFVDR
                     IEGSYSNVVGLPVSKVYEELRRLGFFPLPPAAN"
     gene            2451..2879
                     /locus_tag="PKK95_08710"
     CDS             2451..2879
                     /locus_tag="PKK95_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOC18334.1"
                     /translation="MPKKAVKVGLTIVVLALALGGLLRATLREGTEYYKHVGEVVGAE
                     AEWQGKRLQLHGYVVRDSILRKRDSLEYRFKVTHKVGDSARVVPAFYTGVVPDTFADD
                     AEVVLKGTLQADGFHVAPNGVMAKCPSKYEARKSPASPGA"
     gene            3010..5484
                     /locus_tag="PKK95_08715"
     CDS             3010..5484
                     /locus_tag="PKK95_08715"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOC18335.1"
                     /translation="MASLGTFTLLAAFVVSAYAAAAAVAGARRHSRGLVDSAIGAFYL
                     VAGLMTVASAIIVHAFVTEDYSIKYVQHYSDALQPFVYKLTSYWGGLDGSIMFWVFLL
                     AIFGAVAVWVNRERHRELMPYVVATISLVQMFFLFLMVIHRNPFATFLIETPADGRGL
                     NPLLQNPYMVIHPPALYVGFVGMTIPFAFGMAALITGHLDDSWLRAVRRWTMVSWLFL
                     SFGLTLGMIWAYEVLGWGGYWGWDPVENAGLLPWLTATAFLHSVMVQERRGMLKVWNV
                     TLVIVTFFLTIFGTFMTRSGVVQSVHAFGEDRQLAWMFTVFMAAILAVSFGYLIYRLP
                     ALRARHELDSWVSREAAFLVNNWVLLFAALFVLFATMFPTISEALTGERLTVGPPFFN
                     KWMLPIGLILLFLTGVGPLLAWRKSTLANLRDQFLWAALGFAAAGAGLAALGIPFWPA
                     GLCFALCAFVGVTIVQEFVRGAEVRRQATGTDYLTAMIGLVARSKRRYGGYIVHLGIV
                     LMFVGFAGEAFKVDEQVLLKPGEQSQVGKYVVRNDGVRVTDDGQKQMITAHVAVLRDG
                     KEIDKAYPGRWYYRKHEQEPVSQVAIRRGAAEDLYIVLPGFDLETQSATLQVVVNPLV
                     NWIWVGFGILAFGTMIALLPEQVFAFAAARAPAAAGTSLLLVALVAAPGPAAAQHVED
                     PAFRPVVPKTALEKDLQRSLICMCGTCGRQLVGECTCGYAAEMRAEIANLVKMGLTRE
                     QVLDYYIKKHGSQEALAMPIDSGFNRLAWLLPYVLGAAGLSLVGVAAAKWSRRVSPAA
                     PQVPRAAAPADPQLEQRLDDELRDLD"
     gene            5462..7183
                     /locus_tag="PKK95_08720"
     CDS             5462..7183
                     /locus_tag="PKK95_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18336.1"
                     /translation="MNSETSTDRAGDDRIHGFQPWHLFLVGSLLAASAAALAVRGTAP
                     ANVVFICLTVFGAGWSAYLLYRAFRPLVEGQADEAPEMLGGRTRAALEREKTLTLRAI
                     KELDFDRAMGKVSEADYQEMTARLRGRAVRLMRQLDEGQNVYRQLIEKELASRPPAAE
                     APRKKAGSAGTLVLAVAFALAGGPSNALAQMAGAGGSGMPDPRAMSGIPLPSSDAPAG
                     TVTVRLVRASLTNPIPRHPVQFVLGGGRSQTVDTDENGRATLSGASPGSLVRAVAVVD
                     GERLESQDFEIPSNAGVILLLTASEKGAGGQAAADAVAGTVVLSGQSRIVLEFQDDAL
                     QVFYLFDIVNRAAAPVKVDAPLVFEMPEGAQTTTVLEGSSPQAAASGRRVVVQGPFAP
                     GTTSVHMACTMAPGADARIRLHLPAALDSLLVIAEKAGAMVLHSAQLPEVQESMDAGR
                     RLLVARGPGLQAGDVLALDLSGLPHHPTWPRNVALVLALGVLAAGAWSATRASAGAAA
                     ARARQQLEARRARLLDQVARLDEGHRRGKVTDDEYGERREALLAELERVYGELDFETA
                     PGQEATA"
     gene            7180..7893
                     /locus_tag="PKK95_08725"
     CDS             7180..7893
                     /locus_tag="PKK95_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18337.1"
                     /translation="MKVDFEEVAVRELSRHYGRRRALWRVSLTCRAGEVLGLLGPNGA
                     GKSTLLAILATLLAPSAGEVRYGDIGLRDAGPALRARLGFLSHDLHLYPELTARENLR
                     FFARLYGLDRVAERVGEALAHAGLSERGDDLVSGFSRGMRQRLALERALLHEPRLLLL
                     DEPFTGLDDASVGALVARLRALREAGRIVVVVTHDLDVAEALLDRLAVLKDGRLVALE
                     DASGRIREHYRASIVSRTP"
     gene            7910..8587
                     /locus_tag="PKK95_08730"
     CDS             7910..8587
                     /locus_tag="PKK95_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOC18338.1"
                     /translation="MFFRVAWLVMRKDLTVEVRSREILYTTAFFAVACVLVFSFALVK
                     EGRPLEDAAAGILWIAVAFAGTLALGRTFERERHSETLRALMLAPSPRAAIYVGKLLG
                     IVLLLLVVEAVLVPLIGLLFNVPILARPLLLTGLLAAGTVGFAAVGTLFAAMLVRARS
                     RDVLLPVLLYPITIPVVIGGVRGTAALFELPPSGEVAGMWMALLLFFDVVFVTLSLWT
                     FEPLMTE"
BASE COUNT         1161 a         2994 c         3115 g         1421 t
ORIGIN      
        1 gccggtggtg gtttcgggcg agccgaacat cacgccgatc ttctcgcgca gctggttgat
       61 gaagaccagg cacgtcttcg acttcgagac caccccggtg agcttgcgca gcgcctgcga
      121 catgaggcgc gcctgcaggc ccatctgcgc ctcgcccatc tcgccttcga tctcggcccg
      181 cggcaccagg gcggctaccg agtcgaccac tacgacgtcc acgccgcccg agcgcaccag
      241 cacctcgacg atttcgagcg cctgctcccc gttgtcgggc tgcgagacca gcaggttctc
      301 gaggtccacg ccaagcttct tggcgtaggt cgcgtcgagc gcgtgctcgg cgtccacgaa
      361 ggcggccatc ccgcccagct tctgcgcctg cgcgatcacc tgcagcgcaa gcgtggtttt
      421 gcccgacgac tcggggccgt agatctccac cacccgcccg cgcggcaccc ccccgatgcc
      481 gagggcgaaa tcgaggctga tcgagccggt cgggatcgcg tccaccggcg tgatcgctcc
      541 tttcgacccg aggcgcatga tcgacccctt gccgaactgc ttttcgatct ggctgacggc
      601 cacgtccagg gccttggtgc gttcgttgcg gtcgtcggca ggcatgcgcg gctccttcag
      661 gaaagaggct cagggtcagg gctcacgact cagggctcag ggcggtgagg cggcttcaag
      721 atccgccggc ccagggctcc ggcggcgaac ggattgaagc tccagcattc tagaggcgtg
      781 ccgcccggct gtcaagcgaa aggattggct gaccgtggca caaatcgtct gacatcaact
      841 atttgcagtt gccgcgactt tcgcctcggt ccgcggaggc cgcaccgggt tgcagaaacc
      901 gccgctcggc ggcgtggcgt gtgcgcaggc gttgccacca ccatcccgcc agggcgaacg
      961 gtcccagcag aatcacttcg cgcgcgacgg ccgccaggtt gtgggccagg aaatccggca
     1021 gccagtagcg gcgcgagatg cccgtgaaca ggtcggcacc cgagtggaaa tagcggtccg
     1081 tgaagggcca gcccgccatc accccgtacg gcggcgttcc gtcctctccc agccagtcga
     1141 ggaaggggtg ggtggcatag gcgagcgcga gggtcgcggc gagccggggg tggcggccgc
     1201 ggccggccag ccaggccgcg aagaacacca agaccaccgc gccgatgctg tgcgtccact
     1261 ggctgtgcac gccgaccagg aggtcgaggt cggggagcag cgcgacgccg aggatcagcc
     1321 ccgcccgccg cagggccggc cgccaggccc cggctgcccg gccgctcgtt cccgccttcg
     1381 gttcgggcgc agccaccgcc cagcccgccg cgagcgcccc gagcgcgtga ccgatcgggc
     1441 tcggcatcag gcgcgacccc gtcgaggtgg agacgccagg cgtacctctg cgacgacccc
     1501 cgcgcccacc aaagcctcga gccgccgggc gatctctgga aggtcccggg agatctcgcg
     1561 ggcccaggcc tcgtcggagg cggtcacctc gacgatccgg ccgtcgcacc accgaacggc
     1621 ggtggccctc gagatggccg gccccacggc cgcggtccag gcgaaggcca gcttctcctg
     1681 gtccatcggc ccggctcgca ggagctggcg gatcgcgcgg ggcagcacgg aatgcagcgg
     1741 ttccatcaac gagcattgtg acaggggcca gggctggggg gctagcaggc cgctgaacaa
     1801 cgcggcatga tccccccatg cgcctcatcc tcgcctcgtc ctcgccccgc cgctcggaac
     1861 tgctgacggc cgcccgcatt cccttcgagg tgcggccggt cgaggtggac gaacgcccac
     1921 tcgacggcga agatccggcg gcctacgtcg ttcgcgccgc ccgcgacaag gccggtgcct
     1981 tcggcgcggt ccccccggac acggtcgtgc tgtccgccga cacgacggtc gtggtggagg
     2041 atgagatcct cggcaagccg gccgacgcgg ccgacgcggc gcgcatgctg cgcctgctct
     2101 cgggccgctg gcacgaggtg ctgagtgggg tggttctccg ccacgcgagc ggtgagacga
     2161 cggaggtgga cgttagccgg gtgcggttcc tcccgctctc cgaggatgag gtgcgctggt
     2221 acatcggaac cggggagccg ttcggcaagg ccggggctta cgcgatccag gggctggcct
     2281 ctcgattcgt cgaccgcatc gaggggtcgt actcgaacgt ggtcgggctg ccggtctcga
     2341 aggtctacga ggagctgagg cggctggggt tcttcccgtt gccccccgct gcaaattgac
     2401 ctgccgggtt ttcggcgata ttcttgggag aattcccgcg aaaggtgcgc atgcccaaga
     2461 aggccgtcaa ggttggactg accatcgtcg tgctcgcgct ggcgctcggc gggctgctgc
     2521 gggcgacgct tcgcgagggc accgagtact acaagcacgt tggcgaggtc gtgggggcgg
     2581 aggccgagtg gcagggcaag cgcctccagc ttcacggcta cgtcgtccgc gactccatcc
     2641 tgcgcaagcg cgattcgctc gagtaccggt tcaaggtgac gcacaaggtg ggcgacagcg
     2701 cccgcgtcgt tccggccttc tacaccggcg tcgtgcccga caccttcgcc gacgacgcgg
     2761 aggtggtgtt gaagggaacg cttcaggccg acggtttcca cgtcgctccg aacggcgtca
     2821 tggcgaagtg cccctcgaag tacgaggccc ggaagtcacc ggcctctccc ggtgcgtagc
     2881 cagttgcgac cggggccagg ccggtcgagc gcgatcaacc ccaatccgca tccagtcccc
     2941 ggccgcagcc gtccgccgtc tcccgcgccg cgcgaggcgc gcgatcgggc tgcggtccac
     3001 gagtagacca tggcttcgct tggcaccttc acgctcctcg cggccttcgt ggtctcggcc
     3061 tacgcggcgg cagccgcggt cgccggggcg cgccgccact cgcgcggcct cgtcgacagc
     3121 gcgatcggcg cgttctacct ggtggccggc ctgatgacgg ttgcttcggc gatcatcgtc
     3181 cacgccttcg tcaccgagga ttactcgatc aagtacgtcc agcactactc ggacgcgctc
     3241 cagccgttcg tctacaagct cacctcctac tggggcgggc tggacggctc gatcatgttc
     3301 tgggtgttcc tgctggcgat cttcggcgcc gtcgcggtct gggtgaaccg ggagcggcac
     3361 cgcgagctga tgccgtacgt ggtggccacc atctcgctcg tgcagatgtt cttcctgttc
     3421 ctgatggtga tccaccggaa ccccttcgcg acctttctga tcgagacgcc ggcagacggg
     3481 cgcggcctca acccgctgct ccagaacccc tacatggtca tccacccgcc ggcgctctac
     3541 gtcggcttcg tggggatgac cattcccttc gccttcggca tggccgccct catcaccggc
     3601 cacctcgacg attcgtggct ccgggccgtc cgccgctgga cgatggtcag ctggctgttc
     3661 ctgtcgttcg gcctcacgct cggcatgatc tgggcgtacg aggtgctcgg ctggggcggc
     3721 tactgggggt gggacccggt cgagaacgcg ggcctgctgc cctggctgac ggccaccgcg
     3781 ttcctgcact cggtgatggt gcaggagcgg cgcgggatgc tgaaggtctg gaacgtgacg
     3841 ctggtgatcg tcaccttctt cctgacgatc ttcggcacct tcatgacccg ctcgggcgtc
     3901 gtgcagtcgg tgcacgcctt cggcgaggac cgccagctgg cctggatgtt cacggtgttc
     3961 atggccgcca tcctcgccgt cagcttcggc tacctcatct accgcctgcc ggccctgcgt
     4021 gcgcggcacg agctcgactc ctgggtgtcg cgcgaggcgg ccttcctggt gaacaactgg
     4081 gtgctgctgt tcgcggcgct gttcgtgctg ttcgccacga tgttcccgac gatcagcgag
     4141 gccctcaccg gcgagcggct gacggtcggt ccgccgttct tcaacaagtg gatgctgccg
     4201 atcgggctga tcctcctctt cctgaccggg gtggggccgc tgctggcgtg gaggaagtcg
     4261 acgctcgcca acctgcgcga ccagttcctg tgggcggccc tcggcttcgc ggccgcgggg
     4321 gccgggctcg cggcgcttgg cattccgttc tggccggccg gcttgtgctt cgcgctctgc
     4381 gcgttcgtcg gggtcaccat cgtgcaggag ttcgtgcgcg gcgcggaggt caggcggcag
     4441 gcgaccggca ccgactacct gacggcgatg atcggcctgg tggcccgatc gaaacggcga
     4501 tacggcggct acatcgtcca cctgggcatc gtgctgatgt tcgtcgggtt cgccggcgag
     4561 gcgttcaagg tggacgagca agtcctgctg aagccgggcg agcagtcgca ggtgggcaag
     4621 tacgtcgtgc gcaacgacgg cgtgcgggtg accgacgacg ggcagaagca gatgatcacg
     4681 gcgcacgtcg cggtgctgcg cgacggcaag gagatcgaca aggcataccc ggggcgttgg
     4741 tactaccgga agcacgagca ggagccggtc agccaggtgg cgatccgccg cggggccgcc
     4801 gaggatctct acatcgtcct gccaggcttc gacctcgaga cgcagtcggc gacgctgcag
     4861 gtggtcgtca acccgctggt gaactggatc tgggtcggct tcggcattct ggcctttggg
     4921 acgatgatcg ccctgctgcc cgagcaggtc ttcgcgttcg cggccgcgag ggcgcccgcg
     4981 gcggccggca cgagcctgct gctggttgcg ctcgtcgccg cgcccggccc ggcggcggcg
     5041 cagcacgtcg aggacccggc gttccggccg gtggtgccga agaccgcact cgagaaggat
     5101 ttgcagcggt cgttgatctg catgtgcggc acctgcggcc ggcagctggt gggggagtgc
     5161 acctgcgggt acgcggccga gatgcgtgcc gagatcgcga acctggtgaa gatggggctg
     5221 acgcgcgaac aggtgctgga ctactacatc aagaagcacg gcagccagga agcgctggcg
     5281 atgccgatcg acagcggctt caaccgcctg gcctggctgc tgccgtacgt gctcggtgcg
     5341 gccggcctga gcctggtcgg ggtggcggcg gcgaaatggt cgcgccgcgt gtcgcccgca
     5401 gccccgcagg ttccgcgcgc cgcggcgccg gccgatcccc aactggagca gcgcctggac
     5461 gatgaactcc gagacctcga ctgatcgcgc gggcgacgac cgcatccacg gcttccagcc
     5521 gtggcacctg ttcctcgtgg ggtcgctcct ggcagcgtcg gcggcggcgc tcgcggtgcg
     5581 gggtaccgcc ccggcgaacg tcgtcttcat ctgcctgacg gtgttcggcg ccgggtggtc
     5641 ggcgtacctg ctctacaggg cgttccgtcc cctcgtggaa ggacaggccg acgaggcgcc
     5701 cgagatgctg ggggggcgca cgcgcgccgc cctggagcga gagaagacgc tgaccctgcg
     5761 cgcgatcaag gagctcgact tcgaccgggc catgggcaag gtctcggagg ccgactacca
     5821 ggagatgacc gcgaggctcc gcgggcgcgc cgtccgcctg atgcggcagc tcgacgaggg
     5881 ccagaacgtc taccgccagc tgatcgagaa ggagctggca tcgcgcccac ccgctgccga
     5941 ggcgccgcgc aagaaggccg gctcggccgg gacgctggtg cttgccgtcg cgttcgccct
     6001 ggctggcgga ccgtccaacg ccctcgccca gatggccggt gccggcgggt cggggatgcc
     6061 cgatccgcga gccatgtccg gcatcccgct cccgtccagc gacgcgccgg cggggaccgt
     6121 cacggtgcgg ctggtccgcg ccagcctcac caacccgatt ccccgccacc ccgtccagtt
     6181 cgtcctcgga gggggccgct cccagaccgt cgacaccgac gagaacggac gcgcgacgct
     6241 ctccggcgca tcgcccggct cgctggtgcg ggccgtcgcg gtggtggacg gcgagcggct
     6301 cgagtcgcag gacttcgaga tcccctcgaa cgccggcgtc atcctgctgc tgaccgcgag
     6361 cgagaagggc gctggaggcc aggcggcggc cgatgccgtc gccggtaccg tcgtgctgtc
     6421 gggccagtcg cggatcgtcc tcgaattcca ggacgatgcg ctgcaggtgt tctacctttt
     6481 cgacatcgtg aaccgcgcgg cggccccggt aaaggtggac gcgccgctcg tgttcgagat
     6541 gcccgagggg gcgcagacca ccaccgtgct cgaggggtcg tcgccgcagg cggcggccag
     6601 cggccgccgg gtcgtggtcc aggggccctt cgcacccgga acgacgtcgg tgcacatggc
     6661 gtgcacgatg gcgcctggcg ccgacgcccg gatccggctc cacctgccgg ccgccctcga
     6721 cagcctcctg gtgattgccg agaaggccgg cgcaatggtg ctgcattcgg cgcagctgcc
     6781 ggaggtgcag gagtcgatgg acgcggggcg gcgcctgctc gtcgcgcggg gccccgggct
     6841 gcaggctggt gatgtcctcg cgctcgacct ctcggggctg ccgcaccacc cgacctggcc
     6901 gcgcaacgtg gcgctcgtac tggcactcgg cgtgctggcg gccggcgcct ggtcggcgac
     6961 gcgggcctcg gccggcgctg cggcggcgcg cgcgcgccag cagctcgagg cccgccgggc
     7021 gaggctgctc gaccaggtgg cacggctcga cgagggccac cggcgcggga aggtgacgga
     7081 cgacgagtac ggcgagcgcc gcgaggcgct gctggcggag ttggagcggg tgtacggcga
     7141 gctggacttc gagaccgccc ccggccagga agccaccgcg tgaaggtcga cttcgaggag
     7201 gttgcggttc gcgagctgtc gcggcactac ggcaggcgac gtgctctgtg gcgcgtgtcg
     7261 ctcacctgcc gcgccggcga ggtgctgggg ctgctcgggc cgaacggggc cggcaagtcg
     7321 acgctgctcg cgatcctcgc gacgctcctg gccccttccg cgggtgaagt ccgctacggt
     7381 gacatcggtc tccgcgacgc cggtccggcc cttcgcgccc gcctcgggtt cctgtcgcac
     7441 gacctgcacc tctaccccga gctgaccgcc cgcgagaacc tgcgcttctt cgcccggctc
     7501 tacggcctcg accgggtcgc ggagcgtgtt ggtgaagcgc tggcacatgc cggcctgtcg
     7561 gagcggggcg acgacctggt gtcggggttc tcccggggaa tgcgccagcg cctcgccctg
     7621 gagcgcgcgc tgttgcacga gccgcggttg ctgctgctcg acgagccgtt caccggcctc
     7681 gacgatgcct cagtcggtgc gctggtggcg cggctcaggg cgctgcgcga ggcaggacgg
     7741 atcgtcgtcg tggtgacgca cgacctcgac gtggccgagg cgctgctcga ccggctggcg
     7801 gtcctgaagg acggccggct tgttgccctc gaggacgcga gcgggcggat cagggagcat
     7861 taccgcgcgt ccatcgtgag ccgcacgccg taagccttga gcctggtcta tgttctttcg
     7921 cgttgcctgg ctcgtgatgc gtaaagacct caccgtcgag gtgaggagcc gggagatcct
     7981 ttacacgacg gcgttcttcg cggtcgcgtg cgtgctcgtg ttctcgttcg cgctggtgaa
     8041 ggaggggcgc cccctcgagg acgccgcggc cgggatcctc tggatcgccg tcgcgttcgc
     8101 cggcacgctg gcgctcgggc gcaccttcga gcgcgagcgt cactccgaga cgctgcgcgc
     8161 gctgatgctg gcgccctcgc cgcgggcagc catctacgtc ggcaagctgc tcggcatcgt
     8221 gctgctgctg ctggtcgtcg aggccgtgct cgtgccgctg atcgggctgc tgttcaacgt
     8281 gccgatcctc gctcggccgc tgctgctgac cggactgctg gccgccggga cggtggggtt
     8341 cgccgcggtg ggcacgttgt tcgccgcgat gctcgtgcgg gcgcggagcc gcgacgtgct
     8401 cctgcccgtg ctgctgtacc cgattaccat cccggtcgtg atcggcggcg tgcggggcac
     8461 cgccgcgctg ttcgagctgc ctccctcggg cgaggtggcg ggcatgtgga tggcgctgct
     8521 gctgttcttc gacgtggtgt tcgtgacgct gtcgctctgg accttcgagc cgctgatgac
     8581 cgagtgatcc cgatgaagcg ctcctacccg ttcctcgtgc tcgctgcggt cgtcctcttc
     8641 gccgcggcgc cgatcctcgt cgaccgggcg ccgtacgagt cgacgatggg g
//
LOCUS       DAOPQX010000135         8677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9084_length_8677_cov_4.384134, whole genome shotgun sequence.
ACCESSION   DAOPQX010000135 DAOPQX010000000
VERSION     DAOPQX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9084_length_8677_cov_4.384134"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..445
                     /locus_tag="PKK95_08735"
     CDS             29..445
                     /locus_tag="PKK95_08735"
                     /inference="COORDINATES: protein motif:HMM:NF021676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18339.1"
                     /translation="MQTEREACALYPVGLGDERAMSVIRGNLAKDFGRHRWWLAVNTL
                     AGIGSLALMPLPGPNVIGFYFAFRIVGHFLSVRGARQGLDVVCWRLRESAHLAELGSS
                     LALPPPERERVVAEVSRQLGLRRLPRFFKRMAPNPA"
     gene            461..1495
                     /gene="lpxD"
                     /locus_tag="PKK95_08740"
     CDS             461..1495
                     /gene="lpxD"
                     /locus_tag="PKK95_08740"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOC18340.1"
                     /translation="MKLREIAERLGCRLDGDGEVEIAGVAGIADAGPGDLTFLANPRY
                     THALQTTRASAVILADAGPPAPCAALRSEIPYLAFGRAVRLLNPGPRIEPGVHRLAEV
                     DPTATLGEGVSIGPFAWIGPGAAIGARTVILSHVAVGRGATIGDDCLLHSHVSIRERV
                     VLGRRVIVQDGAVVGADGFGFAKRPDGSYEKIPQPGAVVVEDDVEIGANTTIDRPAVG
                     ETRVCAGAKIDNLVQIAHGVRVGRDTLLCAQVGIAGSSTVGDRVVLAGQVGVAGHLEV
                     GDGVVATAQTGIPNSVEANTLVSGYPAIPNRDWLKASAVFKRLPELKKAVSDLEARLE
                     ALERQLASAK"
     gene            1505..2851
                     /locus_tag="PKK95_08745"
     CDS             1505..2851
                     /locus_tag="PKK95_08745"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC18341.1"
                     /translation="MRLRPTPATSRLPAVLALAALAAAAAGAAPRPPRLNARQVTFEN
                     GLQVVVLEDHSTPIVHAQLWYHVGSKDERPGRTGFAHLFEHMMFKGSKNVRPELHASL
                     ISSVGGESNAYTTEDVTVFWATLPAQYLPLVLWLEADRMASLKVDEATFRREREVVKE
                     ERRMRVENQPYGRLSELIYAHAFTVHPYRHTTVGSMKDLEAASVDDVREFYRTYYVPN
                     NATLALVGDFDADEAIALARRYLGQVPRSPRPVPRERPVEPPQTTLRRVTLHENWPLP
                     VIVVAHHIPPDRHADSFPLRLAAKVLSDGQSSRIYRSLVYEKGHALSAFGTANIAEDP
                     NLFFAVAVVQPGRDLDEAERALVAELERLADEAISERELARARNQLARDHVVGRASNQ
                     EKATHLAHAVVIHGDAASADREFDSLMEATAADVQRAARAYFTPANRLILRVLPPS"
     assembly_gap    2910..3009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3227..4507
                     /locus_tag="PKK95_08750"
     CDS             3227..4507
                     /locus_tag="PKK95_08750"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC18342.1"
                     /translation="MHREQPAVSLRAIVRAGSAHDPPGRPGVAAMVSTLLDQGAAGQS
                     AQQLAEAIDGAGGLLTTGLGRDLSFANAVVMKGDLALGMRLLAGVLRSPSFAPEELER
                     QRQQVLSGLKVALEDPTYLADLVFDRLVYGDHPYGLPGHGTVESVQALTRDDLLAFHG
                     AHYAPNNTLLAVVGDVEVEEAMTQVASAFGDWARRELPPFSPARPPGPARRLVVIDRP
                     DAAQTEIRVGQLGVPRKVDDHVAVDLLIKILGGAGANRLHRVLRIERGLTYSASADME
                     SLKQGGQFVGQTSTRAGTTGEALRLMVDEFARLKRERVGDRELADAKAYLTGHFPLTI
                     ETPEQIATQVLNALFYELPLRDLETYRQRVNVVTLEDVARAALRLQPDRLTMVLVGRA
                     EEIVPQLGRLGFSRFEVIPPGALDVLSADLRRAR"
     gene            4507..5667
                     /gene="lpxB"
                     /locus_tag="PKK95_08755"
     CDS             4507..5667
                     /gene="lpxB"
                     /locus_tag="PKK95_08755"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOC18343.1"
                     /translation="MPATVLISCGEPSGDLYAGALAASLRSLDPGVRLLGLGGDRFEA
                     AGGELVGDYRGLAVTGIAEAVRVLPQSLLMYRRLVGVAREVRPDVAVVIDFPDFNFRL
                     AASLRRLGIPLVYYIPPQLWAWRSGRMRRLQAIANRILVIFPFEPEIYRRAGTEATFV
                     GHPLLELARPTEARETFLSGAGLDPGAPVIALLPGSRPNEVRAILPTLIEATAAISAA
                     EPRAQYVVARAPGLDSGLFAIGERAPLRHVSIVEGRADDVLAACDLVITASGTATVQA
                     AIHERPMVVVYRVSPLTYAVGRRFVHLDTFAMVNLVAGERVVPELIQEAFTPLAVADE
                     ALRLLRDGEARDRALRGVRAVKEKLGGPGASRRAAEAVLELARRPRNVECRM"
     gene            complement(5729..8218)
                     /locus_tag="PKK95_08760"
     CDS             complement(5729..8218)
                     /locus_tag="PKK95_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HOC18344.1"
                     /translation="MKRAAWLVAGIVFFTGIVVLVPRAQRGAPPPARPGVLGAGVTLL
                     PNGWKIAPAGEHLAVGDLPLNMVMAPGGRYLIVTNNGYAKPTLTVVDLENRFVKARVP
                     VDHAWLGLAWHPDGRRLYSSGAGQNTVNEFTWADDRLTLTGSHVLGRPERYAGSDYRE
                     NAGFIGGVTVAADGKTLYAVHVMGTALSAVDLERNRVRQTIPLPAEPYTCLLSPDGQT
                     LFVSLWGGGKVLLFDSATLQQTGEIEVGQHPNAMQLSADGRRLFVACANTNAVWVVDL
                     DDRTAREQISVSLYPKAPVGSTPNALALSPDGKTLLVANADNNTVAVVDVAESGRSEV
                     EGFIPTGWYPTGVQFTPDGKRIVVLSGKGLTSMANPRGPTGDFQYSGSMLQGSLSIVP
                     APDAQTLAAMTKKVYELVRYTEANRLAPAEAPKASPIPARVGQPSPIKYVFYVIRENR
                     TYDQVLGDMPRGNGDPNITLFGKAITPNAHAMAEEFVLFDNFYVDAEVSMDGHAYSTA
                     AYATDVVEKIWPTNYGGRGAIYLSEGGWTMRGPFGNFAAPPEGYIWDFAKRAGVTVRS
                     YGEFADWKEKWGPVEATVPGLQGLVHPTYPPYDTFIPDNKRVDIWLEEFRKFEADGQL
                     PRLNIIRLGNDHTNGTRPDSPTPRAMVAENDYAVGRVVEAISKSRFWKESAIFIIEDD
                     AQNGPDHVDAHRSVLLMISPFARRGAVDSTLYTTSGVLRTMELILGIPPMSQYDAAAS
                     PMYNAFQATPVLTPFTLRPAQYPFDEMNAPNAPGAQASLAMNLEEADMAPELELNEII
                     WKSVHGAAARMPPPVRTGFVRAIAEEEEDEK"
     gene            8364..>8677
                     /locus_tag="PKK95_08765"
     CDS             8364..>8677
                     /locus_tag="PKK95_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18345.1"
                     /translation="MRKALIAAWVAPLSLLVAAPLPAAVLVPAELGELAREAAVIAHG
                     RVIAVQGVWTAGRRSIETVVTLEVATVLKGQPGREVSLRVPGGELGRYRSVMVGAPVF
                     REG"
BASE COUNT         1182 a         2960 c         3090 g         1345 t
ORIGIN      
        1 gataaatcag cgcctcttag atgatgctgt gcagacggag cgcgaggcgt gcgccctcta
       61 cccggttggg ttgggcgacg agcgggcgat gtcggtcatc cggggcaacc tcgcgaaaga
      121 cttcgggcgg caccgctggt ggctggcggt caacacgctg gccgggatcg ggtcgctcgc
      181 gctgatgccg ctgccgggcc cgaacgtgat cggcttctac ttcgcgttcc ggatcgtcgg
      241 ccacttcctc tcggtgcgcg gcgcgcggca ggggctcgac gtggtgtgct ggcgcctgcg
      301 cgaaagcgct cacctggccg agctgggatc atcgctggcc ctgccgcctc ccgagcgcga
      361 gcgagtggtg gccgaggtgt cgcgacagct cgggctgcgg cgcctgccgc ggttcttcaa
      421 gcgcatggcc ccaaaccccg cgtgatatac tcacccggcc ttgaaactca gggaaatcgc
      481 cgagcgcctc ggctgccgcc tcgacggcga cggggaggtg gagatcgcgg gcgtggccgg
      541 catcgctgat gcggggcccg gcgacctgac cttcctcgcc aacccgcgct acacccacgc
      601 cctgcagacg acgcgcgcct cggcggtgat cctcgccgac gccggcccgc ccgccccgtg
      661 cgcggcgctg cggagcgaaa tcccctatct cgcgttcgga cgcgccgtga ggctgctgaa
      721 ccccggcccg cggattgagc ccggcgtgca ccgcctggcg gaggtggacc caacggccac
      781 tctcggcgag ggcgtctcga tcgggccgtt cgcctggatc gggcccgggg ctgcgatcgg
      841 cgcccgcacc gtcatcctgt cgcacgttgc ggtcgggcgc ggtgccacca tcggcgacga
      901 ctgcctcctg cactcccacg tctcgatccg cgagcgcgtc gtgctcggcc ggcgcgtcat
      961 cgtgcaggac ggggccgtgg tcggggccga cgggttcggg ttcgccaagc ggcccgacgg
     1021 gtcgtacgag aagatcccgc aacccggcgc cgtggtcgtc gaggacgacg tggagatcgg
     1081 cgccaacacg accatcgacc gcccggcggt gggcgagacg cgcgtgtgtg ccggggcgaa
     1141 gatcgacaac ctcgtgcaga tcgcgcacgg cgtccgcgtc gggcgcgaca cgctcctctg
     1201 cgcgcaggtc ggcatcgcgg gcagctcgac ggtgggagac cgcgtcgtgc tcgccgggca
     1261 ggtcggcgtg gcggggcacc tcgaggtcgg cgacggcgtc gtcgcgaccg cccagacggg
     1321 gatcccgaat tcggtcgagg ccaataccct ggtgtcgggg tacccggcga tcccgaaccg
     1381 cgactggctc aaggcgtcgg ccgtcttcaa gcgcctgccg gagctgaaga aggcggtctc
     1441 cgacctcgag gcccgcctcg aggcgctcga gcgacagctc gcctccgcca agtagcagga
     1501 cctcatgcgg ttgcgcccga cgccggccac ctcgcggctg ccagcggtgc tcgcgctcgc
     1561 cgcgctggcg gcggccgcag ccggcgcggc ccctcggcct cctcggctca acgccaggca
     1621 ggtcacgttc gagaacggcc tccaggtggt cgtgctcgag gaccactcca cgccgatcgt
     1681 ccacgcgcag ctctggtatc acgtgggatc gaaggacgag cgtccgggcc ggaccggttt
     1741 tgcgcacctg ttcgagcaca tgatgttcaa gggctcgaag aacgtgcgcc ccgagctgca
     1801 cgcgtcgctc atctcgtcgg tggggggcga gagtaacgcc tacacgaccg aggacgtgac
     1861 ggtcttctgg gcgacgctgc ccgcccagta cctgccgctg gtactgtggc tcgaggccga
     1921 ccggatggcc tcgctcaagg tggacgaggc gacgttccgg cgcgagcgcg aggtggtgaa
     1981 ggaagagcgg cggatgcgcg tcgagaacca gccgtacggc cggctgtcgg agttgatcta
     2041 cgcgcacgcg ttcacggtgc acccgtaccg gcacaccacg gtcggcagca tgaaggatct
     2101 cgaggccgcc tcggttgacg acgtccgcga gttctaccgg acgtactacg tgccgaacaa
     2161 tgccacgctc gcgctggtcg gcgacttcga cgccgacgag gcgatcgcgc tggcgcgccg
     2221 gtacctcggg caggtgccgc gctcgccgcg cccggttccc cgcgagcgtc cggtcgagcc
     2281 gcctcagacc acgcttcgcc gggtgacgct gcacgagaac tggccgctgc cggtcattgt
     2341 cgtggcgcac cacatcccgc ccgaccgaca cgccgattcg ttcccgctgc ggctggcggc
     2401 gaaggtcctc tcggacgggc agagctcgcg catctaccgg agcctggtgt acgagaaggg
     2461 gcacgccctc tccgccttcg ggaccgcgaa catcgccgag gacccgaacc tgttcttcgc
     2521 cgtcgccgtg gtgcagcccg gccgcgacct ggacgaggcc gagcgcgcgc tggttgccga
     2581 gttggagcgg ctcgcggacg aggcgatctc cgagcgggag ctggcgcggg ccaggaacca
     2641 gctggctcgc gaccacgtgg tcgggcgcgc gtcgaaccag gagaaggcga cgcacctggc
     2701 gcacgccgtc gtgatccacg gcgacgccgc cagcgccgac cgggagttcg actcgctgat
     2761 ggaggcgacg gctgccgacg tgcagcgcgc cgccagggcc tacttcacgc ccgccaaccg
     2821 cctgatcctg cgcgtgctgc cgccgtcatg atcgcgctgc ccgccgtttg cgcgctcgcc
     2881 gtgctcgccc tgccagtggc acaggcgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnc ccgcctgtgt cagtagcaca ggcgtccccg cctgtgtcag tagcacaggc
     3061 gtccccgcct gtgtcagtag cacaggcgtc ctcgcctgtg tcagcagtgc tccaggcttc
     3121 aaggtggccg tcgagcaccc cgccccgtcc gctggccccc agggacattc gctttccggc
     3181 gtacgaggtc aggacgctcc cgaacgggct gcaggtggtg gtggtcgtgc atcgtgagca
     3241 gcctgccgtg agtctccgcg ccatcgtgcg ggcgggcagc gcgcacgacc cgccgggacg
     3301 gcccggcgtg gccgcgatgg tttccacgct gctcgaccag ggcgcagccg ggcagagcgc
     3361 gcagcagctg gccgaggcga tcgacggggc gggcggcctg ctgaccaccg gcctcgggcg
     3421 cgacctctcg ttcgcgaacg cggtcgtcat gaagggcgat ctcgcgctcg gcatgcgact
     3481 gctggccggc gtgcttcgga gcccgtcctt tgcaccggag gaactggagc ggcagcggca
     3541 gcaggtgctg tcgggactca aggtggccct cgaggatccg acgtacttgg ccgacctggt
     3601 gttcgatcgc ctcgtctacg gcgaccaccc gtacggcctc cccggccacg ggaccgtcga
     3661 gtcggtgcag gcgttgacgc gcgacgacct gctggcgttc cacggcgcgc actacgcgcc
     3721 caacaacacg ctgctcgcgg tcgtcggtga cgtggaggtc gaggaggcga tgacgcaggt
     3781 cgcctcggcc ttcggagact gggcccggcg cgagctgccg cctttctcac ccgcccggcc
     3841 gcccggcccg gcgcgccggc tggtggtgat cgatcgcccg gacgcggcgc agaccgagat
     3901 ccgcgtgggc cagctcggcg ttccgcggaa ggtggacgac cacgtcgcgg ttgacctgct
     3961 gatcaagatc ctcggtgggg ctggcgccaa ccggctgcac cgcgtgctgc gcatcgagcg
     4021 gggcctcacc tactcggcgt cggccgacat ggagagcctg aagcagggcg gccagttcgt
     4081 cggccagacc agcacgcgcg ccgggacgac cggcgaagca ctgcggctga tggtggacga
     4141 gttcgcgcgg ctgaagcgcg agcgcgtcgg cgaccgcgag ctcgccgacg cgaaggcgta
     4201 tctgaccggg cacttcccgc tgacgatcga gacgcccgag cagatcgcga cgcaggtgtt
     4261 gaacgcgctg ttctacgagc tgcctctccg cgacctcgag acctaccggc agcgggtgaa
     4321 cgtcgtgacc ctcgaagacg tggcccgcgc ggcgctccgc ctccagccgg accgtcttac
     4381 catggtcctg gtgggccggg cggaagagat cgttccccag ctcggacggc tcggattctc
     4441 ccggttcgag gtgattccgc caggcgcgct ggacgtcctg tcggccgacc tccggagggc
     4501 tcgctagtgc cggccactgt tctcatctcc tgcggggagc cgtcgggaga cctgtacgcg
     4561 ggcgctctgg cggcgtcgct tcgctcgctc gacccgggcg tgcgcctctt gggactcggg
     4621 ggcgaccggt tcgaggcggc aggcggcgag ctggtgggcg actaccgcgg gctggcggtg
     4681 acggggattg ccgaagcggt cagggtgctg ccgcagtcgc tgttgatgta ccggcgcctg
     4741 gtcggcgtgg cccgagaggt tcgccccgac gtggcggtgg tgatcgactt ccccgacttc
     4801 aacttcaggc tggcggcgtc actccggcgg ctgggcatcc cgctcgtcta ctacatcccc
     4861 ccgcagctgt gggcctggcg aagcggccgc atgcgccggc ttcaagcgat cgccaatcgc
     4921 atcctcgtga tcttcccctt cgagccggaa atctaccgga gggcgggaac ggaggccacc
     4981 ttcgtcggcc acccgctgct ggaactcgcg cgtccgaccg aagcacgcga gaccttcctg
     5041 agcggcgcgg gcctcgaccc cggtgcgccg gtgatcgcgc tgctgccggg cagccgcccc
     5101 aacgaggtgc gcgccatcct gccgacgctg atcgaggcga ccgccgcgat ctcggcagcc
     5161 gagccgcgcg cccagtacgt cgtggcacgg gcgcctggcc tcgactcggg cctgttcgcc
     5221 atcggggagc gggcccccct ccgccacgtc tccatcgtcg agggccgggc cgacgacgtg
     5281 ctggccgcgt gcgacctggt gatcacggcg tcggggaccg ccacggtgca ggcggccatc
     5341 cacgagcggc cgatggtggt ggtgtaccgg gtctcgccgt tgacgtacgc ggtgggccgc
     5401 cgcttcgtgc acctcgatac cttcgcgatg gtgaacctgg tggcggggga gcgtgtcgtg
     5461 cccgagctga tccaggaagc gttcacgccg ctggcggtgg cagacgaggc gcttcgcctg
     5521 cttcgcgacg gcgaggcgag agaccgcgcg ctccgcggcg tgcgcgcggt caaggagaag
     5581 ctcggggggc caggggcgag caggcgagcc gcggaggcgg tgctggaact ggcccgccga
     5641 ccgcgaaatg tagaatgcag gatgtagatg gagaggggcg gttcgtgccg ccccttttct
     5701 acatcttcct tctacacttc atcttcttct atttctcatc ttcctcttct tccgcgattg
     5761 ccctcacgaa gccggtgcgg acgggtggcg gcatgcgtgc ggccgcaccg tgcaccgact
     5821 tccagataat ctcgttcagc tcgagttcgg gagccatatc ggcttcctcg aggttcatcg
     5881 cgagcgacgc ctgcgcgcct ggtgcgttcg gcgcgttcat ctcgtcgaac gggtactgcg
     5941 ccgggcgcag cgtgaacggc gtgagcaccg gggtcgcctg gaaggcgttg tacatcggcg
     6001 aggccgcggc gtcgtactgg ctcatcggcg ggatcccgag gatcagctcc atcgtgcgga
     6061 gcacgcccga ggtcgtgtag agggtcgagt ccactgcgcc gcgccgcgcg aacgggctga
     6121 tcatcagcag caccgagcgg tgcgcgtcca cgtgatcggg cccgttctgc gcatcgtcct
     6181 cgatgatgaa gatggccgac tccttccaga aacggctctt cgagatggcc tccacgacac
     6241 gccccaccgc gtagtcgttc tcggcgacca tggccctcgg cgtgggcgag tcgggccgcg
     6301 tgccgttggt gtggtcgttc cccagccgga tgatgttcag gcgcggcagc tggccgtcgg
     6361 cctcgaactt ccggaactcc tcgagccaga tgtccacccg cttgttgtcg gggatgaagg
     6421 tgtcgtaggg cgggtaggtc gggtggacca gcccctgcag cccggggacg gttgcctcga
     6481 ccggtcccca cttctccttc cagtcggcga actcgccgta gctgcgaacc gtgacgccgg
     6541 cgcgcttggc gaagtcccag atgtacccct caggcggcgc ggcgaagttg ccgaacgggc
     6601 cgcgcatcgt ccagccgccc tcgctcaggt agatggcgcc gcggcctccg tagttggtgg
     6661 gccagatctt ctcgaccacg tcggtcgcgt acgccgcggt ggagtaggcg tggccatcca
     6721 tgctgacctc ggcgtccacg tagaagttgt cgaagaggac gaactcctcg gccatggcgt
     6781 gggcgttggg ggtgatcgcc ttgccgaaca gcgtgatgtt cgggtcgccg ttgccgcgcg
     6841 gcatgtcgcc gagcacctga tcgtaggtgc ggttctcgcg gatcacgtag aagacgtact
     6901 tgatggggga gggctggccg acgcgcgccg ggatcggcga cgccttcggc gcctcggccg
     6961 gggccagccg gttcgcctcg gtgtaccgca cgagttcgta gaccttcttg gtcatcgcgg
     7021 ccagggtctg cgcatcaggg gccggcacga tcgagagcga cccctgcagc atcgatcccg
     7081 agtactggaa gtcgccggtc gggccgcgcg ggttggccat actcgtcagc cccttgccgc
     7141 tgagcacgac gatccgcttc ccatcggggg tgaactggac gcccgtcggg taccaaccgg
     7201 tcgggatgaa gccctccacc tcgctccggc cgctctcggc gacgtctacc acggcgacgg
     7261 tgttgttgtc ggcgttcgcg accagcagcg tctttccgtc gggcgagagc gcgagcgcgt
     7321 tcggcgtgct gcccaccggc gccttggggt agagggacac ggagatctgc tcgcgcgccg
     7381 tccgatcgtc gaggtccacg acccagacgg cgttcgtgtt cgcgcacgcc acgaacaggc
     7441 ggcggccgtc ggccgagagc tgcatcgcgt tcgggtgctg gcccacctcg atctcaccgg
     7501 tctgttgaag ggtcgcggag tcgaacagca ggaccttccc gccgccccac agcgagacga
     7561 acagcgtctg cccgtcgggg gagaggagac aggtgtaggg ctcggccggc agcgggatcg
     7621 tctgccgcac ccggttgcgc tcgagatcga cggccgagag cgcggtgccc atcacgtgca
     7681 ccgcgtagag cgtcttgccg tccgcggcca ccgtcacgcc gccgatgaag ccggcgttct
     7741 cgcggtagtc ggagccggca taccgctcgg gccgcccgag cacgtgcgag ccggtgagcg
     7801 tcagccggtc gtcggcccag gtgaactcgt tgaccgtgtt ctgcccggcg cccgaggagt
     7861 acagccggcg gccgtcggga tgccacgcga ggccgagcca ggcgtggtcc accggcacgc
     7921 gcgccttcac gaaccggttc tccagatcga cgacggtcag ggtcggcttg gcgtacccgt
     7981 tgttggtgac gatcaagtac cgcccgccgg gagccatcac catgttgagg ggcaggtcgc
     8041 caaccgccag gtgctcgccg gccggggcga tcttccagcc gttcggcagc agggtgacgc
     8101 cggcgcccaa aacccccggc cgcgcgggcg gcggcgcgcc gcgctgcgcc ctgggaacca
     8161 gcaccacgat gcccgtgaag aagacaattc ccgcaaccag ccacgccgct cgcttcatgt
     8221 gcccccctcc gccggccgat cctactggac aaccggcggg cggcgcaaag gagctcccgt
     8281 caccttggcg gtgcccgcgg ccgataaggg agggtgtgac acgttttcgc gccacctttt
     8341 cgtgctgatg gtagagtgct gacatgcgca aagccctgat cgccgcctgg gtggcgccgc
     8401 tgtcgctgct cgtcgcggcg ccgctgcccg ccgccgtgct cgtgccggcg gagcttggcg
     8461 agctggcgcg cgaggcggcg gtgatcgcgc acggccgggt gatcgcggtc cagggagtgt
     8521 ggacggccgg gcgccgaagc atcgaaacgg tggtcaccct ggaggttgcg accgtgctca
     8581 aggggcagcc cgggcgcgag gtgagcctgc gggttcccgg cggcgagctg gggcggtacc
     8641 gaagcgtgat ggttggcgcg ccggtgttcc gcgaggg
//
LOCUS       DAOPQX010000136         8670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9094_length_8670_cov_4.777365, whole genome shotgun sequence.
ACCESSION   DAOPQX010000136 DAOPQX010000000
VERSION     DAOPQX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9094_length_8670_cov_4.777365"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2087
                     /locus_tag="PKK95_08770"
     CDS             <1..2087
                     /locus_tag="PKK95_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOC18346.1"
                     /translation="RSGDLLRPSWSPDSKWIAYTKRLQNYMGAVFLYSIDGAKSTQMT
                     DGMSDARAPVFDAGGKYLYFAASTDAGASLQPDIHSFSRPMSGSLYLVVLSKDEPSPF
                     APESDEEPLDAAKKEEPARSADAKPEGESETKPEEAKEAKAESGPARRSGEATKAEGK
                     GTKPPEPVKIELDGILQRVLSMPMPARRYVSLQAGKPGVLLAVEAPAPFGGGGQTGLT
                     VHRYDLKARRSDVAIGGVNSFEVTRNGEKMLYRQEDRWYIAALRPMPPPGAPASPPPP
                     APPPSRALATDAIEVRVDPRAEWRQMYREAWRIQREFFYDPGFHGLDLEAAGKRYEPF
                     LERVASRSDLNYLFAEMLGDITVSHLGVGGGALPDVRRVQTGLLGADFSTDAGRYRFA
                     RVYSGENWNPQLQAPLTRPGVNVTAGEYLLAVNGRPVTTAQSVYSFFEGTVGKSVRLR
                     VGPNPTEAGSREVIVVPVASEQPLRNYAWIEDNRRYVDKVSGGRIAYIYMPDTAGGGY
                     TNFNRYFYAQVGKEGLIVDERFNGGGNLATDIVEMLGRPVMSMVATRDAENEVQPQGA
                     IFGPKVMLVNEMAGSGGDAMPWYFRKAGLGPLVGKRTWGGLVGRAAGVPLIDGGYVGA
                     PSSAVWDPATSQWIAENVGVAPDVEVEQDPALVRQGKDPQLDKAIEMVMAELAKKTPF
                     APKRPAYPVYTR"
     gene            2182..3348
                     /locus_tag="PKK95_08775"
     CDS             2182..3348
                     /locus_tag="PKK95_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC18347.1"
                     /translation="MMKNLFAIGLFAAAAVSAGAQAPPAAQQQPAPPEKMRVEVRPGS
                     ARQQIPMDPERARVLYVPTDPALQSPGRNYQADIEGRLATEARYAAACKGVIAYQKVS
                     YRSRIGDMDIPAYLFQPLQKRGPRGHAAMIWVHGGVHGNWGITMFPFVKEAIERGYVV
                     IAPEYRGSIGYGEAHHQAIDYGGYEVDDTMSAYDYLKSLPHVDPDRVGMMGWSHGGYI
                     TLLSVFRDEHPLKAGAAIVPVTNLLYRLSRYGPRYQWDFSTQKRIQGLPFEKPELYIE
                     RSPMYHVDRLKVPLLVHVATNDRDVYYDEDQMIVDALRSRKPDLAETKTYVDPDPWGP
                     SVGHSFSRRVDAQTLERVDSPAQIDSWNRTWVFFEWILRPYEDRSKPAPPVRTR"
     gene            3464..4138
                     /locus_tag="PKK95_08780"
     CDS             3464..4138
                     /locus_tag="PKK95_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18348.1"
                     /translation="MHSRSTPVAGRAHHPGRVLAATLTVALASAATALAGPPLLCHPF
                     DIGSARSLPWDGRSSWFDVDASYALNSLVADTEALLAPSTPVIVRMETLRRAAIYAGR
                     DAAVARQLLDRLAARAVGSAAKEPDPLALFDAGYLVEALRQLTMVPQFKAHRAALEPL
                     VAGKDGYVMVKKSLALRPNDASLEFGAALVVADKDQRAYRSHADRARAGANGDALLAK
                     NIRQLG"
     gene            4191..4733
                     /locus_tag="PKK95_08785"
     CDS             4191..4733
                     /locus_tag="PKK95_08785"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC18349.1"
                     /translation="MGQAPAIRAVDDSDLVRAAQGGDRAAFGLLYERYGAMVHGVLLA
                     RVRWEEVDDLLQDVFLSALERLSSLRDAGAFGGWLAAIARNRATDHHRRKVATDSGGA
                     EEVEARGNGPSAEALAALEAIQRLPAAYRETLTLRLVEGMTGPEIAARTGLTPRSVRV
                     NLHRGMKKLREELEVKRGTR"
     gene            4730..5758
                     /locus_tag="PKK95_08790"
     CDS             4730..5758
                     /locus_tag="PKK95_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18350.1"
                     /translation="MSGDYLWDRSGRPDPDVAALESVLSELRHRGGPLALVPPAPSRQ
                     APARTRNWRSLAVPLALAASIAVLVAGTWFARPQRGAPAAAVSAGSWKAAPVSGSARV
                     ASEPIEAEVHVPPGRWLETGPGASARLSAGEIGTLRVGPLSRLRVISDAPGEHRLALA
                     RGSLDAFIWASPRQFFIETPSAVAVDLGCAYRLEVDEDGTGRLRVTLGWVGFELDGRE
                     SLVPRGAMCTTRPGRGPGTPHFEDASAGFRAALASLDDGGAPAAGDVLDRLLREARPR
                     DALSLWHLLSRLDRLSAAKVYDRLATLAPPPPQASKDLVLAGDRPALDAWWDSLGLAP
                     VSRYRELR"
     gene            5835..6776
                     /gene="thyX"
                     /locus_tag="PKK95_08795"
     CDS             5835..6776
                     /gene="thyX"
                     /locus_tag="PKK95_08795"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOC18351.1"
                     /translation="MTANQEQIVRASVPALDAILGQHFPVLDDGFVRAVDYMGDDGAI
                     VQAARVSYGKGTRRVREDRHLIRYLMRHAHTTPFEMCELKLHVRVPMDAWRQWIRHRT
                     ASVNEYSTRYSEAIDAAQRTPAGGWRAQSSSNRQGSADLLPNETGARLSAEEEELHRR
                     AREIYQGRIAAGVAREQARKDLPLSTYTEAYWKIDLHNLLHFLHLRMDLHAQQEIRAY
                     ATVIGEQIVARWVPIAWEAFLDYRRSALTLSGSETEIVAAIGAGDRAKATELAAAAGW
                     LERTEEGALKASRERRELEEKLLRLGMTAPWKERAVR"
     gene            6888..8210
                     /locus_tag="PKK95_08800"
     CDS             6888..8210
                     /locus_tag="PKK95_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18352.1"
                     /translation="MTRFAILMLVLHLAAAPAAAQDSAFSGPAGPAAISSASSLDALK
                     VFLDCKDHTPCYHDYVRVEIDYVEYVNVREAADVHVLVTSLGTGAGGREFALKFIGQG
                     RFRGVDDEVLYASLPTDTEEGVRQGFVRTLKLGLVRYVLHTAVRDRLSVLHEDAAGGP
                     GQQARSRVPNDDPWDHWVLRARFNAYYESEASASSSNLNGSVSANRVTDAWKFSLSSY
                     RDTRRSEYTFSDGTFYVSTRRGYDVTGQAIRSLGGHWAAGGKAFIAASTYENKDRVLN
                     ATGAIEYNVFPYAESTRRQLTLQYLVGITAFDYNEETVLGKLSETVPRHTFRTAFDMK
                     QTWGSLSADLDVSQYLHDTSMNRRTVSAEMDVRLFRGFALNLAGRASSIHDQLYLPVG
                     EASAEEVIARQRQLATSFRRSFRVGVSYTFGSIFSSVVNTRLSNFVGG"
     gene            8334..>8670
                     /locus_tag="PKK95_08805"
     CDS             8334..>8670
                     /locus_tag="PKK95_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18353.1"
                     /translation="MSQLARVLPLPVVLFTLLLGLDGVALLQAQGPVTSPEQFFGFRM
                     GADRKIARWDRLIEYYELLEKQGAGKVKVVDMGPTEMGNRFLLVIVTSAANQKNLEQL
                     RLNTLRLSDP"
BASE COUNT         1345 a         3152 c         2958 g         1215 t
ORIGIN      
        1 gccgatcggg cgacctgctg cgcccctcct ggtcccccga ctcgaagtgg atcgcctaca
       61 ccaagcggct gcagaactac atgggcgccg tcttcctcta ctcgatcgac ggcgcgaaga
      121 gcacgcagat gaccgacggg atgagcgacg cccgcgcccc ggtcttcgac gcgggcggca
      181 agtacctcta cttcgccgcc agcaccgatg ccggggcctc gctgcagccc gacatccaca
      241 gcttcagccg tcccatgtcg ggcagcctgt acctggtggt gctgtcgaag gacgagccct
      301 cgcccttcgc ccccgagagc gacgaggagc ccctcgacgc ggcgaagaag gaggagcccg
      361 cccggagcgc tgacgcaaag cccgaagggg agagcgaaac gaagccggaa gaggcgaagg
      421 aagcgaaggc cgagagcgga ccggcccgcc gcagcggcga ggccacgaag gcggagggta
      481 agggaaccaa gccgcccgag cccgtgaaga tcgagctcga cgggatcctg cagcgcgtgc
      541 tctcgatgcc gatgcccgcc cgccgctacg tctccctcca ggctgggaag cccggcgtcc
      601 tgctggcggt cgaggccccg gccccgttcg gcggcggcgg ccaaaccggc ctcaccgtcc
      661 accgctacga cctcaaggcg cggcgctccg acgtggcgat tggcggcgtc aacagcttcg
      721 aggtgacccg caacggcgag aagatgctct accgccagga agaccggtgg tacatcgccg
      781 ccctgcgccc gatgccgccg ccgggggccc ccgcttcacc gccaccgccc gcgccgccgc
      841 cttcccgcgc gctcgccacc gacgcgatcg aggtccgggt cgatccacgc gccgagtggc
      901 ggcagatgta ccgcgaggcc tggcgcatcc agcgcgagtt cttctacgat cccggtttcc
      961 acggcctcga tctcgaggcc gccgggaagc gatacgagcc gttcctcgag cgtgtcgcct
     1021 cgcgcagcga cctcaactac ctgttcgccg agatgctcgg cgacatcacg gtcagccacc
     1081 tcggcgtggg aggcggcgcg ctgcccgacg tccggagggt tcagaccggc ctgctcgggg
     1141 ccgacttctc gaccgacgcc ggccgctacc ggttcgcccg cgtctacagc ggcgagaact
     1201 ggaacccgca gctccaggct ccgctcacgc ggcccggcgt caacgtcacc gccggtgagt
     1261 acctgctggc cgtgaacggc cggccggtca cgaccgccca gagcgtctac tcgttcttcg
     1321 aagggacggt cggcaagagc gtgaggctgc gcgtcggtcc caatcccacc gaagcaggct
     1381 cgcgcgaggt gatcgtggtg cccgtggcga gcgagcagcc gctccgcaac tacgcctgga
     1441 tcgaggacaa ccgccgctac gtcgacaagg tcagcggggg ccggatcgcc tacatctaca
     1501 tgcccgacac ggccggcggc ggctacacga acttcaaccg ctacttctac gcgcaggtcg
     1561 gcaaggaggg cctgatcgtg gacgagcggt tcaacggcgg cggcaacctg gccaccgaca
     1621 tcgtcgagat gctggggcgg ccggtgatga gcatggtcgc cacgcgcgac gccgagaacg
     1681 aagtgcagcc gcagggggcg atcttcggcc cgaaggtgat gctcgtcaac gagatggccg
     1741 ggtcgggcgg cgacgcgatg ccgtggtact tccgaaaggc gggcctcggg ccccttgtcg
     1801 gcaagcgcac gtggggcggc cttgtgggcc gcgccgccgg cgtgccgttg atcgacggcg
     1861 gctacgtcgg ggcgccctcg tctgcggtct gggacccggc gaccagccag tggatcgccg
     1921 agaacgtcgg cgtcgccccc gacgtcgagg tcgagcagga tccggccctc gtccgccagg
     1981 ggaaagaccc ccagctggac aaggccatcg agatggtgat ggccgagctc gcgaagaaga
     2041 cgcccttcgc accgaagcgc ccggcctacc cggtttacac gcgataggga agcgggtcgc
     2101 ggccctgaag ctcaccgccg gctgtcacgc tgaggttggt atagtgtgcg gcgtgcggtt
     2161 cgtgcgcctc agaggacgac catgatgaag aacctgttcg ccatcggctt gttcgccgcc
     2221 gccgccgtct cggccggcgc gcaggccccg cctgccgccc agcaacagcc cgccccgccc
     2281 gagaagatga gggtcgaggt ccggcccggt agcgcgcgcc agcagatccc catggatcct
     2341 gagcgggcgc gggtgttgta cgtcccgacc gaccccgcgc tccagtcgcc cgggcggaac
     2401 taccaggccg acatcgaggg gcgtctcgcg accgaggccc gctacgcggc cgcctgcaag
     2461 ggcgtcatcg cctaccagaa ggtgagctac cgcagccgca tcggcgacat ggacatcccg
     2521 gcctacctgt tccagccgct ccagaaacgg gggccgaggg ggcacgccgc gatgatctgg
     2581 gtgcacggcg gcgttcacgg gaactggggc atcaccatgt tccccttcgt gaaggaggcg
     2641 atcgagaggg gctacgtcgt catcgcgccc gagtaccgcg gcagcatcgg ctacggcgag
     2701 gcgcaccacc aggcgatcga ctacggcggc tacgaggtgg acgacacgat gtcggcgtac
     2761 gactacctga agtcgctgcc gcacgtcgat cccgatcggg tcgggatgat ggggtggagc
     2821 cacggcggct acatcacgct gctctcggtg ttccgcgacg agcacccctt gaaggccggc
     2881 gccgcgatcg tgcccgtcac caacctcctg taccgcctgt cgcgctacgg cccgcgctac
     2941 cagtgggact tctcgacgca gaagcgcatc caggggctgc cgttcgagaa gcccgagctg
     3001 tacatcgagc gttcgccgat gtaccacgtc gacaggctga aggtgcccct cctggtgcac
     3061 gtggcgacca acgaccgcga cgtctactac gacgaggacc agatgatcgt cgacgcgctg
     3121 cggtcgcgga agcccgacct ggccgagacg aagacgtacg tggaccccga cccgtggggc
     3181 ccgagcgtgg gccactcctt cagccggcgc gtggacgccc agacgctcga gcgcgtggac
     3241 tcgcccgcgc agatcgactc gtggaaccgc acctgggtct tcttcgagtg gatcctccgc
     3301 ccctacgagg atcgctcgaa gccggccccg cccgtccgga cgagataagg gcccaatcag
     3361 gaaatcggca aacgtcgtta acacctgacg gcgtgggggc gtcctcttca cagcgaggca
     3421 catgtctcgc cgcgaaacgc gccacgggcg cgaggaggcc cccatgcaca gccgttccac
     3481 ccccgtcgcc ggccgcgcgc accaccccgg ccgcgtgctc gccgccacgt tgaccgtcgc
     3541 cctggccagc gcggcgaccg cgctcgccgg cccgccgctg ctctgtcacc ccttcgacat
     3601 cggttcggcg cgatccctgc cctgggacgg cagatcgtcc tggttcgacg tcgacgcgtc
     3661 ctacgcgctg aactcgctcg tcgccgacac cgaggcgctg ctcgcgccgt ccaccccggt
     3721 gatcgtgcgg atggagacgc tccgccgcgc ggccatctac gccgggcgcg acgccgccgt
     3781 cgcccggcag ctgctcgatc ggctcgccgc ccgcgccgtg ggctcggcag ccaaggagcc
     3841 cgacccgctc gcactcttcg acgccggcta cctggtcgaa gcgctgcgtc agctgacgat
     3901 ggtgccgcag ttcaaggcgc accgcgcggc actcgagccg ctcgtcgccg gcaaggacgg
     3961 ctacgtgatg gtgaagaaga gcctcgcgct gcggcccaac gacgcgtcgc tcgagttcgg
     4021 cgcggcgctg gtggttgccg acaaggacca gcgcgcgtac cgctcgcacg ccgaccgggc
     4081 ccgcgcgggt gccaacgggg acgcgctgct ggcgaagaac atcaggcagc tgggatagtc
     4141 cggacctcgt gagattgccg gggagcgtcg tgttgggtca tgctctggtc ttgggccagg
     4201 cgccagccat ccgtgccgtg gacgacagcg acctcgtgag agccgcccag ggcggcgacc
     4261 gcgccgcctt cggcctgctc tacgagcggt acggcgcgat ggtgcacggc gtgctgctcg
     4321 cgcgtgtcag gtgggaggag gtggacgacc tgctgcagga cgtcttcctc tctgccctcg
     4381 agcggctgtc atcgctccgc gacgccggcg ccttcggcgg ctggctggcg gcgatcgccc
     4441 gcaaccgcgc caccgaccat caccgccgca aggtggcaac cgattccggc ggcgcggagg
     4501 aggtcgaggc gcgaggcaac ggaccgagcg ccgaggcgct ggccgcgctc gaggccatcc
     4561 agcggctgcc ggcggcctat cgggaaacgc tgacgctcag gctggtcgag ggaatgaccg
     4621 ggcccgagat cgcggcccgc acgggtctca cacctcggtc ggtgcgcgtc aacctgcaca
     4681 ggggaatgaa gaagttgcgc gaggagctcg aggtgaagcg gggaacgcga tgagcggcga
     4741 ttacctctgg gatcgctcgg gcaggcccga cccggacgtg gcggcgctgg agagcgtgct
     4801 gtcggagctt cgccaccgcg gcgggccgct ggcactcgtc ccgccggccc cgtcgcgcca
     4861 ggcaccggcg cgaacacgaa actggcgctc gctggccgtt ccgctggcgc tcgcggcatc
     4921 aatcgcggtg ctggtggccg gcacctggtt cgccaggcct cagcgggggg cgccggccgc
     4981 cgccgtctct gccggctcgt ggaaggccgc cccggtcagc ggcagcgcgc gggtcgccag
     5041 cgagccgatc gaggccgagg tgcacgttcc acccggccgc tggctcgaaa ccggccccgg
     5101 cgcgtccgcc cggctgagcg ccggcgagat cggcaccctt cgcgtcggtc cgctctcgcg
     5161 cctccgcgtg atcagcgacg cccccggaga acaccgcctg gcgctggccc ggggcagcct
     5221 cgacgcgttc atctgggcgt cgccgcggca gttcttcatc gagacgccct cggcggttgc
     5281 ggtcgatctc ggctgcgcct accgcctcga ggtcgacgag gacggcaccg gccgcctacg
     5341 ggtgaccctc ggctgggtgg gcttcgagct ggacggccgc gaatcgctgg tgccgagggg
     5401 tgccatgtgc acgacgcgtc ccggccgcgg accggggaca ccccacttcg aggacgcgag
     5461 cgcgggcttc cgtgcagcgc tggcctccct cgacgacggt ggagcgccgg ccgccgggga
     5521 cgtcctcgac cgtctgctgc gcgaggcgcg cccccgcgac gcgctttccc tctggcacct
     5581 cctgtcgcgc cttgaccggc tttcggccgc caaggtctac gaccgcctcg cgacgctcgc
     5641 cccgccgccc ccgcaggcat ccaaggacct ggtactggcg ggcgatcggc cggctctcga
     5701 cgcctggtgg gacagcctcg gcctcgcccc ggtgtcgcgc tatcgcgagc tgcgataggg
     5761 ccgccgcaag ggtgccgcga gagaaggcgc ccccggtctc caccgggtgc ggcaaagcac
     5821 gtacaatgtc ctgcgtgact gccaaccagg agcagatcgt ccgcgcgtcg gtcccggcgc
     5881 tcgacgccat cctcggccag catttcccgg tcctcgacga cgggttcgtt cgcgccgttg
     5941 actacatggg cgacgacggc gcgatcgtgc aggcggcgcg cgtgtcgtac ggcaagggca
     6001 cccggagagt gcgcgaggat cggcacctca tccgctacct gatgcgccac gcgcacacca
     6061 cgcccttcga gatgtgcgag ctgaagctcc acgtccgcgt cccgatggac gcctggcggc
     6121 agtggatccg ccaccggaca gcgagcgtca acgagtactc gacccgctac tccgaggcga
     6181 tcgacgctgc ccagcgcacg ccggcggggg gctggcgggc gcagtcctcg tccaaccggc
     6241 agggcagcgc cgaccttctg ccgaacgaga ccggggcgcg cctctcggcg gaggaggagg
     6301 aactgcaccg ccgcgcgcgc gaaatctacc aggggcgaat cgcggccggc gtggcgcggg
     6361 aacaggcgcg caaggacctg ccgctctcca cctacaccga ggcctactgg aagatcgacc
     6421 tccacaacct cctgcacttc ctgcacctgc ggatggacct gcacgcgcag caggagatcc
     6481 gcgcctacgc gaccgtgatc ggcgagcaga tcgtcgcccg gtgggtgccg atcgcgtggg
     6541 aggccttcct cgactaccgc cgcagcgcgc tgaccctctc ggggtcggag accgagatcg
     6601 ttgccgcgat cggtgcgggc gaccgcgcga aggcgaccga gctggcggct gcggccggat
     6661 ggctcgagcg aaccgaagag ggcgccctca aggcgagccg cgagcggagg gagctcgagg
     6721 agaagctcct gcggctcggc atgacggcgc cgtggaagga aagagcggta agataggggc
     6781 cgccccaccc cgggcccggc gcgtgcccct ggggcatgcc cttccgtctc cgacaacagc
     6841 cgacccggcg agccgcacgc ccccgcgctc tgcccgcgcg aggtgtgatg acgcgtttcg
     6901 ccatcctgat gctggtgctg cacctcgcgg ctgcacccgc cgccgctcag gactccgcat
     6961 tctcgggtcc cgcgggcccc gcggcgatct cgtccgcctc gtcgctcgac gccctcaagg
     7021 tgttcctcga ctgcaaggac cacacgccct gctaccacga ctacgtcagg gtggaaatcg
     7081 actacgtcga gtacgtcaac gtgcgcgaag cggccgacgt tcacgtgctg gtcacctcgc
     7141 tcggcaccgg cgccggcggg cgcgagttcg ccctcaagtt catcggccag gggcggtttc
     7201 ggggtgtgga cgacgaggtg ctctacgcgt cgctgccgac cgacaccgaa gagggcgttc
     7261 gccaggggtt cgtccggacg ctgaagctgg gcctcgtccg ctacgtgctg cacaccgccg
     7321 tccgcgaccg cctcagcgtg ctgcacgagg acgcggccgg cggtcccggc cagcaggccc
     7381 gttcgcgcgt ccccaacgac gacccctggg accactgggt cctgcgcgcc cgcttcaacg
     7441 cctactacga gagcgaggcg tcggcctcgt cgagcaacct gaacggatcg gtctcggcga
     7501 accgggtgac cgacgcctgg aagttcagcc tgtcgtcgta ccgggacacg cggcgcagcg
     7561 agtacacctt cagcgacggg accttctacg tcagcacgcg caggggctac gacgtgaccg
     7621 gccaggccat caggagcctg gggggccact gggcggcggg cggcaaggcc ttcatcgccg
     7681 cctccaccta cgagaacaag gaccgcgtgc tcaacgccac cggcgcgatc gagtacaacg
     7741 tcttccccta cgccgagtcc acgcggcggc agctcacgct gcagtacctg gtcggcatta
     7801 cggccttcga ctacaacgag gagaccgtcc tcggcaagct gtccgagacc gtgccgcgcc
     7861 acacctttcg caccgccttc gacatgaagc agacgtgggg atcgctgagc gccgacctcg
     7921 acgtctccca gtacctgcac gacacctcga tgaaccgccg cactgtttcc gccgagatgg
     7981 acgtccggct gttccgcggc ttcgcgctca acctggcggg ccgcgcctcg agcatccacg
     8041 accagctgta tcttcccgtg ggcgaggcga gcgccgaaga ggtgatcgcg cgtcagcgcc
     8101 agctcgccac ctcgttccgg cgatcgttcc gcgtgggcgt gagctacacc ttcggctcga
     8161 tcttcagctc ggtggtcaac accaggctct cgaacttcgt cggcggatag cttccaggac
     8221 cagcaccctc ggcaccttca gcaccgcacc ttcagcacct ccggcaccct cagcaccccc
     8281 agcacgacac cccgtggggt atgatccccc cgcgtccccc cggagccacc ttcatgagcc
     8341 agcttgcccg cgtcctcccg ctgccggtcg tccttttcac gctcttgctc ggtctcgacg
     8401 gtgtcgcact gctgcaggcc caggggccgg tgacctcgcc cgagcagttc ttcggctttc
     8461 gcatgggagc cgaccgcaag atcgcccgct gggacaggct gatcgagtac tacgagttgc
     8521 tcgagaagca gggcgccggc aaggtgaagg tcgtggacat ggggcccacg gagatgggca
     8581 accggttcct gctggtcatc gtcacttccg ccgccaacca gaagaacctc gagcagctgc
     8641 gcctgaacac gctcaggctc agcgacccgc
//
LOCUS       DAOPQX010000137         8631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9141_length_8631_cov_4.624650, whole genome shotgun sequence.
ACCESSION   DAOPQX010000137 DAOPQX010000000
VERSION     DAOPQX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9141_length_8631_cov_4.624650"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1074)
                     /locus_tag="PKK95_08810"
     CDS             complement(118..1074)
                     /locus_tag="PKK95_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOC18354.1"
                     /translation="MTRHITRGVVLVALVLAAVPAAALAQQQVVRVTAREAEIRTRPD
                     ARSPMIGRAVTGTVLQVVATERNWYLVVIPPHVRLVPTAPDQGYVDAKLVALLPPGDP
                     RATVKPPATGAAQAGEPGAPAARGAAAGPGLRWRIFGTFAYERFQASRSFEAVYDSAN
                     APSFGGGLELLAGRHLFVQGEFAYLQRTGERVFVFEDEVFPLGIGQKLKLSTIAVNGG
                     YRFGGPASALTPYLGAGAVVAFYNERATGTTAGGEDFSESGFGGQALGGVEFRISRWV
                     AAAVEGRYRYIPGILGDDGVSKEFSEDNLGGGAVAVKLVFGR"
     gene            complement(1116..2549)
                     /locus_tag="PKK95_08815"
     CDS             complement(1116..2549)
                     /locus_tag="PKK95_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC18355.1"
                     /translation="MTKGSRPTAVLASLGLVLAAALALAGGAGQSASAPQGLKQSWLR
                     QASRPATASEFIRPARPRPAAEVRYVPGRVVVRFDADLRAREFRSLAREAGAIAVRRP
                     SWADFHYVEIPPDADPVAAAERLRAQPGIARAEPDPIVRPLWRPNDQYFNLQWHLQRV
                     GMEEAWELNQGGSSRITVAVIDSGVAFVNQGAFAQAPDLAGTRFVPGYDFIWDDDVPT
                     DTDGHGTHVAGTIAQTTNNSVGLAGIAFNVGIMPIKVLTSDWDEENNAPNPPSMSVLA
                     RGIRFAVDHGARVINLSLGADAESFTVESALRYAVGQGAFVAAAAGNSGDEGNAPEWP
                     GAYAKDIDGMMAVGAVDYNLERAPYSTHRDYVEIAAPGGDNDADQNDDDYGDGILQQT
                     LDPDYQERGIFNRFAYMFYQGTSMATAHVSALAALLMDQGITNPAAVEAAIKRFASHK
                     PPGGRSDDLGYGVIDPRASLLGLGLAK"
     gene            2602..3168
                     /locus_tag="PKK95_08820"
     CDS             2602..3168
                     /locus_tag="PKK95_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOC18356.1"
                     /translation="MSPLKDVTSVAPALLLSMPQLDDPNFRHTVVLLCQHSAEGAWGL
                     ILNRPTGTPAVQAVKMEPPVKAANSLDLWVGGPVEPERGCILMGDDPEDEEAVEVCRG
                     IYISGSASLLRRLMEDEQAPARTRLLMGYAGWGPGQLDAELRDSAWLISDVDLGLVFD
                     VDAPLMWEKAIRRLGADPGSLQMSQGVH"
     gene            complement(3314..4360)
                     /locus_tag="PKK95_08825"
     CDS             complement(3314..4360)
                     /locus_tag="PKK95_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18357.1"
                     /translation="MTDRGGAFHPVIELTLARLREFLREPEALFWVVFFPVLLAFALG
                     LAFSRSGEPEVHVGLVPGPGDARVREALLSAPGLVVHDLSREAADRALRDGEVALVVR
                     PGSPPGYRFDAAREESRLARLAVDAALQRAAGREDRFEPRIERVEIPGSRYVDWVIPG
                     LLGMNIMGTGMWALGYAVVYARSRRLLKRLAATPMSRAHYLAAQMLARLVFLGLEVGA
                     LLLFARFVLDVPIAGSLVLVAAVSLLGALSFAGLGMLVASRARTIEAVSGWMNFTMVP
                     MWVLSGVFFSSAHFPNVTQPLINALPLTALIDALRAVMLDGAGFGGVAGEAAILAAWT
                     VGCFALALRLFRWQ"
     gene            complement(4353..5111)
                     /locus_tag="PKK95_08830"
     CDS             complement(4353..5111)
                     /locus_tag="PKK95_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18358.1"
                     /translation="MIDAAWRETPPPAVVCRGLHKRYGEVVAVDGLDLEVRRGECFGL
                     LGPNGAGKTTTIEILEGLMPPDAGEVEVLGLRWGRHARALRQRLGIQLQETQLSEKLT
                     VEETIRLFRSFYHRGPTVDEVVKLVELESKRGSRVGKLSGGQRQRLSVACALVNQPDV
                     LFLDEPTTGLDPQSRRHLWAILLEFRSRGGTVLLTTHYMDEAQAVCDRVGIVDQGRLI
                     AIDTPEALVRGLGEPGKTLEDVFVALTGRHLRDD"
     gene            5149..5859
                     /locus_tag="PKK95_08835"
     CDS             5149..5859
                     /locus_tag="PKK95_08835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOC18359.1"
                     /translation="MAQARTILIAAPELIPALRERIGGDEEMLTFSDAEPMRALQAIA
                     RQCPPLIVLERLFAASPRGAALITRLKADPALDQSQIVIMAHDSDYRRVSPRRKRGAS
                     GAVRVAPAAVRAASGAVRAASGAVRAAKPRLDTGTRRAPRWKMRMGVTVAVDDEVAGL
                     VDLSVLGAQVLASEALKPGQRVEVRFEPGRTGLVVPAEIVWVKVEIAGRRPRYRAGVE
                     FRRAAAQALTAFAETHKA"
     gene            complement(5876..7168)
                     /locus_tag="PKK95_08840"
     CDS             complement(5876..7168)
                     /locus_tag="PKK95_08840"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOC18360.1"
                     /translation="MNVAVIGAQWGDEGKGKIVDLLTPHFQVVARYQGGHNAGHTVLA
                     GGTRFVLHLIPSGILHPGIRCVIGNGVVVDPDALFQEIETLAEYGIEVGDRLVISDKA
                     HVILPYHRDLDLLNEARRGDRKIGTTSRGIGPAYEDKVGRRGIRVGDLFEATSLAEQV
                     RENVHARNRLVGDSALEWRPVVEHLLAHGERMRPWVTDVSVFLAEAMKRGERVMFEGA
                     QGTLLDIDHGTYPYVTSSNSTVGGACTGLGVPPRAVTGVLGVAKAYTTRVGGGPLPTE
                     LTGPMAERLRETGQEYGASTGRPRRCGWYDAVTVRYSVRVNGLDSLALTKLDVLDGLP
                     QILVCTSYKCGDQVLTEFPGDVRVLATCRPIYETMPGWSSPTRGVRRFDELPAEARAY
                     VRRLEELSGVPVGIVSTGSEREDTVFREGSIAASWLAG"
     gene            7277..>8631
                     /gene="hutH"
                     /locus_tag="PKK95_08845"
     CDS             7277..>8631
                     /gene="hutH"
                     /locus_tag="PKK95_08845"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOC18361.1"
                     /translation="MILLDGRSLSLEQLLAVADRGEKVGVSPEAAGRVRAARAVVDAL
                     AGGAAPVYGINTGFGSFAEVKIEPASLDRLQLNLLRSHAAGVGGPLPVRTVRAVMTLR
                     ANVLARGFSGIRLQTLETLVEAINRRVHPVVPARGSVGASGDLAPLAHLALVLVGEGE
                     AWVDGRRAPGAEALRAARLDPVRLTAKEGLALINGTQASTGVLGLALVGAERLARAAD
                     AVAAMTTDALFGSARPFDPRIHAERPFAGQAASAAAIARLLEGSGINASHAGCGRVQD
                     AYSERCAAQVHGSAREAMRFVRELVTVEANAATDNPMVFAADGEILSGGNFHGAPIAM
                     AADLLAIAIAQLATISERRTERLVNPALSELPAFLTREGGLQSGFMLAQVTAAALTSE
                     LKGLAHPASVDTIPTSANKEDHVSMSMGAALKADRALDLARHVLAVELLCACQAIEMR
                     AP"
BASE COUNT         1235 a         3153 c         3063 g         1180 t
ORIGIN      
        1 gcgccacccg gcgtgcggct tccaccagcc gctgcaggtc gggcctgtcg gcgaagcgcc
       61 acatcacgcg gcgcacggcg tcgcccggca gggcattcgg ttccatcgga tcccccgtca
      121 ccgtccaaac accagcttca cggccactgc cccgcccccg aggttgtcct cggagaactc
      181 cttcgacacg ccatcgtcgc cgaggatccc cgggatgtat cgatagcgtc cctcgacggc
      241 cgcggcgacc catcgcgaga tccggaattc caccccgccc agggcctgac cgccgaagcc
      301 gctctcgctg aagtcctcgc cgcccgccgt ggtgccggtt gcccgctcgt tgtagaaggc
      361 gacgacggca cccgcgccaa ggtacggggt gagggcgctg gccggcccgc cgaaccggta
      421 gccgccgttc acggcaatcg tggacagctt cagcttctgg ccgatgccca gcggaaacac
      481 ctcgtcctcg aagacgaaca cccgctcgcc ggtgcgctgc aggtaggcga actcgccctg
      541 cacgaagagg tggcgcccgg ccagcagctc gagcccgccg ccgaacgacg gcgcgttggc
      601 gctgtcgtag accgcctcga acgagcgcga cgcctggaag cgctcgtacg cgaacgtgcc
      661 gaagattcgc caccgcagcc ccggcccggc cgccgcgccc ctggccgcgg gcgctcccgg
      721 ctcgccggcc tgcgcggcgc ccgtcgcggg gggcttgacg gtcgccctcg gatcgccggg
      781 cggcagcagc gccaccagct ttgcatcgac atagccctgg tcgggggccg tcggcaccag
      841 gcggacgtgc ggcgggatga cgacgaggta ccagttcctc tcggtcgcaa cgacctgcag
      901 caccgtgccg gtgaccgccc gcccgatcat cgggctgcgc gcgtcggggc gcgtgcggat
      961 ctccgcctcg cgggcagtga cccggaccac ttgctgctgc gcgagcgccg cggcgggaac
     1021 ggccgccagc accagggcca ccaggacgac gccgcgagtg atgtgtctgg tcatctgctt
     1081 cgatgctccg tgtcggccga ggtcgtgcgc ggcacctact tcgccagccc cagccccagg
     1141 agggacgccc gcggatcgat cacgccgtat ccaaggtcgt cgctgcgtcc gccgggcggc
     1201 ttgtgcgagg cgaaccgctt gatcgccgcc tcgacggccg ccgggttggt gatcccctga
     1261 tccatcagca gggccgcgag cgcgctgacg tgcgccgtgg ccatcgaggt accctggtag
     1321 aacatgtagg cgaagcggtt gaagatgccc cgctcctggt agtcgggatc gagcgtctgc
     1381 tgcaggatcc cgtcgccgta gtcgtcgtcg ttctggtcgg cgtcgttgtc cccgccgggt
     1441 gccgcgattt cgacgtagtc gcggtgcgtc gaatacgggg cgcgctccag gttgtagtcc
     1501 accgcgccca cggccatcat cccgtcgatg tccttcgcat aggcgcccgg ccactcgggg
     1561 gcgttcccct cgtcgccgct gttgccagcc gcggcggcga cgaaggcccc ctggccgacc
     1621 gcgtagcgga gcgcgctctc cacggtgaag ctctccgcgt cggcgcccag gctcaggttg
     1681 atgacccggg caccgtgatc gacggcgaag cggatgccgc gcgcgagcac cgacatgctc
     1741 ggcgggttcg gcgcgttgtt ctcctcgtcc cagtcgctgg tcaacacctt gatcggcatg
     1801 atcccgacgt tgaaggcgat gcccgccagc ccgacgctgt tgttggtcgt ctgggcgatc
     1861 gtcccggcga cgtgggtccc gtgcccgtcg gtatcggtgg gcacgtcgtc gtcccagatg
     1921 aagtcgtagc cgggcacgaa gcgggtgccc gccaggtcgg gcgcctgggc gaacgccccc
     1981 tggttgacga atgcgacgcc gctgtcgatc acggcgacgg tgatgcgcga ggagccgccc
     2041 tggttcaact cccaggcctc ctccatcccg acgcgctgaa ggtgccactg caggttgaag
     2101 tactggtcgt tcggccgcca gagcggacga acgatcgggt cgggctcggc gcgcgcgatg
     2161 cccggctgag cgcgcagccg ctcggcggcc gcgaccgggt cggcgtcggg cgggatctcc
     2221 acgtagtgga agtcggccca cgaggggcgc cgcacggcaa tcgcacccgc ctcgcgcgcc
     2281 aggcttcgga actcgcgcgc gcgcaggtcg gcgtcgaacc gcaccacgac gcggccgggc
     2341 acatagcgca cttccgcggc cggccgcggc cgcgccggcc ggatgaactc ggaagcggtg
     2401 gcgggccggc tggcctgccg cagccacgac tgcttcagcc cctggggcgc gctcgccgac
     2461 tgccctgccc cgcccgcgag ggcgagcgcg gcggccagga cgagcccgag cgaagcgagc
     2521 acggcggtcg gtcgcgaacc cttggtcata ctgtctcccc gcgcgagtgt atcacttcgc
     2581 cacccgcgct atgatgaggg catgagcccg ctgaaggacg tcacctcggt cgcgccggcc
     2641 ctgctgctgt cgatgccgca gctcgacgat ccgaacttcc gccacaccgt cgtcctgctc
     2701 tgccagcact ccgcggaagg cgcctggggc ctgatcctca accggccgac cgggacgccc
     2761 gccgtccagg ccgtcaagat ggagccgccg gtgaaggcgg ccaactcgct cgacctgtgg
     2821 gtgggcggcc cggtggagcc ggaacggggc tgcatcctga tgggcgacga ccccgaggac
     2881 gaggaggcgg tcgaggtgtg ccgcggcatc tacatctcgg gctccgcgtc gcttctacgg
     2941 cggctcatgg aggacgagca ggcgcccgcc cgcacccgcc tcctcatggg atacgcgggt
     3001 tggggacccg gccaactgga cgcggaactg cgcgattcgg cctggctgat cagcgacgtc
     3061 gacctgggac tcgtgttcga cgtggacgcg ccgttgatgt gggagaaggc catccgccgc
     3121 ctcggcgccg accccggctc gctccagatg agccagggag tgcactaaag gctcaaggct
     3181 caaggctcaa gggcttgccc cgccggagcc gcagcctgcc tcgccggagc cgcagattgc
     3241 ctcgccggag ccgcgaacga agtgagcgct tggcggaggc gggaacgaag tgagcgcttg
     3301 gcgaaggcgg gccttactgc cagcggaaca ggcgcagcgc cagcgcgaag cagccgacgg
     3361 tccaggccgc gaggatcgcc gcctcccccg cgacgccccc gaacccggcc ccgtccagca
     3421 tcacggcacg cagcgcgtcg atcagcgccg tgagcggcag cgcgttgatc agcggctgcg
     3481 tcacgttggg gaagtgcgcc gacgagaaga acactcccga cagcacccac atcggcacca
     3541 tcgtgaagtt catccagccc gagacggcct cgatcgtcct cgcgcggctg gcgaccagca
     3601 tcccgaggcc ggcgaacgag agggccccga gcagcgagac ggccgcgacc agcacgagcg
     3661 acccggcgat cggcacgtcg aggacgaagc gcgcgaacag caggagcgcg ccaacctcga
     3721 ggccgaggaa gacgagccgc gccagcatct gcgccgccag gtagtgggcc cgcgacatcg
     3781 gcgtcgccgc caggcgcttc agcaggcggc ggctgcgggc gtagaccacc gcgtagccga
     3841 gcgcccacat ccccgtgccc atgatgttca tgccgagcag gccggggatc acccagtcca
     3901 cgtagcgcga gcccgggatc tccactcgct cgatccgggg ctcgaagcgg tcttcccgcc
     3961 ccgcggcccg ctgcagggcg gcgtccacgg ccaaccgtgc aaggcggctc tcttcccgcg
     4021 cggcgtcgaa tcggtacccg ggcggcgaac cgggccgcac gacgagggcg acctcgccgt
     4081 cacgcagggc gcggtcggca gcctcgcgtg acaggtcgtg gacgacgagg cccggcgccg
     4141 acaggagggc ctcgcgcacc ctcgcgtcgc cgggtcccgg caccaggccc acgtgaacct
     4201 cgggctctcc cgaccggctg aacgcgaggc cgagcgcgaa ggcgagcagc accgggaaga
     4261 acacgaccca gaagagcgcc tcgggttcgc gcagaaactc ccgcagccgg gcgagcgtca
     4321 gctcgatcac ggggtggaag gcgccgccgc ggtcagtcat cgcgcaggtg ccggcccgtg
     4381 agggcgacga agacgtcctc cagggtcttg cccggctcgc ccagcccccg gaccagcgcc
     4441 tcaggggtgt cgatcgcgat gagccgtccc tggtcgacaa tgccgacgcg gtcgcagacc
     4501 gcctgcgcct cgtccatgta gtgcgtcgtc agcagcaccg ttccgccccg cgaccggaac
     4561 tcgagcagga tggcccagag gtggcggcgc gactgcgggt cgaggccggt ggtgggctcg
     4621 tcgaggaaga gcacatcggg ctggttgacc agcgcgcagg ctaccgacaa ccgctggcgc
     4681 tggccgcccg acagcttccc gacccggctg ccgcgcttcg actcgagttc gaccagcttc
     4741 acgacctcat cgaccgtcgg cccgcggtgg tagaaggagc ggaacaggcg gatggtctcc
     4801 tcgacggtga gcttctcact gagctgggtt tcctggagct ggatgcccag gcgctggcgg
     4861 agcgcgcggg cgtgccggcc ccaccggagc cccagcacct ccacctcgcc cgcgtctggc
     4921 ggcatcaaac cctcgaggat ctcgatcgta gtggtcttcc ccgccccgtt cgggccgagc
     4981 agcccgaagc actcaccacg gcgcacctcg aggtcgaggc cgtccaccgc cacgacctcg
     5041 ccgtaccgct tgtggaggcc gcgacagacg acggcggggg gaggggtttc ccgccaggcc
     5101 gcatcgatca cctcgtgatt gtgacacaag gtgtacactg atgagccgat ggcgcaggca
     5161 cggaccatcc tgatcgctgc cccggaactg attcccgccc tccgcgagcg catcggcggc
     5221 gacgaggaga tgctgacctt ctcggacgcg gagccgatgc gggcgctgca ggccattgcg
     5281 cggcagtgcc ctcctttgat tgtgctggag cggctgttcg cggcaagccc gcgcggggcc
     5341 gccttgatca cgcgcctgaa ggccgacccc gcgctcgatc agagccagat cgtgatcatg
     5401 gcgcacgaca gcgactaccg ccgggtctcg ccgcgccgca agcgcggggc atcgggggcc
     5461 gtgcgcgtcg cgccggcagc tgtgcgcgcg gcgtcgggag ccgtgcgcgc cgcgtcggga
     5521 gccgtgcgcg ccgcgaaacc ccgcctcgac accgggacga ggcgcgcgcc ccggtggaag
     5581 atgcggatgg gcgtgaccgt ggccgtggac gacgaggtgg cggggctggt ggacctgtcg
     5641 gtgctgggcg cgcaggtgct ggccagcgag gcgctgaagc ctggccagcg cgtggaggtg
     5701 cgcttcgagc ccggccggac cggcctcgtg gtgccggccg agatcgtctg ggtgaaggtg
     5761 gagattgccg gccgccggcc gcgctaccgg gcgggcgtgg agttccggcg ggcggcagcc
     5821 caggcgctca ccgcgtttgc cgaaacgcac aaggcctgaa ggccaggacc cgttctcagc
     5881 ctgccagcca gcttgccgcg atcgacccct cgcggaacac ggtgtcctcg cgctccgacc
     5941 cggtcgacac gatccccacc ggaacgccgc tcaactcctc gagccggcgc acgtaagccc
     6001 gcgcctcggc gggcagctcg tcgaagcgcc ggacgccgcg tgtggggctg ctccagcctg
     6061 gcatcgtctc gtagatcggg cggcaggtgg cgagcacccg gacgtcgccg gggaactcgg
     6121 tcagcacctg gtcgccgcac ttgtacgagg tgcagacgag gatctgcggg aggccgtcga
     6181 gcacgtcgag cttggtgagg gcgagcgaat cgaggccgtt cacgcgcacc gagtagcgca
     6241 ccgtcaccgc gtcgtaccac ccgcagcgcc gcggacgccc ggtcgaggcg ccgtattcct
     6301 gcccggtctc gcgcaggcgc tcggccatcg ggccggtcag ctcggtcggc agcggcccgc
     6361 cgccgacgcg cgtcgtgtag gccttcgcca cgccgaggac gccggtcacg gcccgcggcg
     6421 gcacgccgag gcccgtgcag gccccgccca cggtggagtt cgacgaggtc acgtagggat
     6481 aggtgccgtg gtcgatgtcg agcagcgtgc cctgggcgcc ctcgaacatc acccgctcgc
     6541 cgcgcttcat cgcctcggcc aggaacaccg agacgtcggt cacccacggc cgcatccgct
     6601 cgccgtgcgc cagcaggtgc tccaccaccg ggcgccactc gagcgccgag tcgccgacga
     6661 ggcggttgcg cgcgtggacg ttctcgcgca cctgctcggc cagcgaggtc gcctcgaaca
     6721 ggtcgcccac gcggatgccc cggcgcccga ccttgtcctc gtacgcgggg ccgatgccgc
     6781 gcgaggtcgt cccgatcttc cggtcgcccc gcctggcctc gttgagcagg tcgaggtcgc
     6841 ggtggtacgg caggatcacg tgggccttgt cgctgatcac cagccggtcg cccacctcga
     6901 tgccgtactc cgccagcgtc tcgatttcct ggaacagtgc gtcgggatcg acgacgacgc
     6961 cgttgccgat gacgcagcgg atcccggggt gcaggatgcc cgacgggatg aggtgcagca
     7021 cgaagcgcgt gccgcccgcc agcaccgtgt gcccggcgtt gtggccgccc tggtaccgcg
     7081 cgacgacctg gaagtgcggc gtgagcaggt ccacgatctt gcccttgccc tcgtcgcccc
     7141 actgcgcacc gatcacggca acgttcatgc gccccttcgc tccttctgaa accggtcaat
     7201 cctagcacat cggatcccgc gtcagacggg acgagggccc ggattagaat ggagtgtttg
     7261 ccccccgagg tggtcgatga tcctgctgga tggtcgctcg ttgtcgctcg aacagttgct
     7321 cgccgtcgcc gaccgcggcg agaaggtggg cgtttcgccc gaggccgccg gccgagtcag
     7381 ggcggcgcgc gcggtggtgg acgccctggc cggcggcgcc gccccggttt acggcatcaa
     7441 caccggcttc gggtcgttcg cggaggtcaa gatcgagccg gcctcgctcg accgcctgca
     7501 gctgaacttg ctgcgaagcc atgccgccgg cgtgggcggc ccgctgccgg tccgcacggt
     7561 gcgggcggtg atgacgctgc gggccaacgt cctcgcccgc gggttctccg gcatccgcct
     7621 ccagacgctc gagacgctcg tcgaggcgat caaccggcgc gtccatcccg tcgtccccgc
     7681 ccgcggatcg gtgggcgcga gcggcgacct cgccccgctg gcccacctcg cgctcgtgct
     7741 ggttggcgag ggcgaggcgt gggtggacgg ccggcgcgcg ccgggcgccg aggcgctgcg
     7801 ggcggcacgc ctggaccccg tccgactgac cgcgaaggag gggctggcgc tcatcaacgg
     7861 gacgcaggcc tcgaccgggg tgctcggcct cgccctcgtg ggcgccgagc ggctcgcccg
     7921 cgccgccgac gcggtggccg cgatgaccac cgacgcactg ttcggttcgg cccgcccgtt
     7981 cgacccgcgc atccatgccg agcgcccctt cgcgggccag gcggcgtcgg cggccgccat
     8041 cgcgcgcctg ctcgagggca gcgggatcaa cgcctcgcac gccgggtgcg ggcgggtgca
     8101 ggacgcctac tccgagcgat gcgcggcgca ggtgcacggc tcggcccgcg aggcgatgcg
     8161 cttcgtgcgc gagctcgtga ccgtcgaggc caacgccgcc accgacaacc cgatggtctt
     8221 cgccgccgac ggcgagatcc tctccggggg gaacttccac ggcgcgccga tcgccatggc
     8281 ggccgacctg ctcgccatcg cgatcgcgca gctcgcgacg atcagcgagc ggagaaccga
     8341 gcgcctcgtc aacccggcgc tgagcgagtt gccggccttc ctcacccgcg aaggggggct
     8401 tcagtcgggc ttcatgctgg cgcaggtgac cgccgccgcg ctcacctcgg agctgaaggg
     8461 gctcgcgcat ccggccagcg tcgacaccat cccgacctct gccaacaagg aagatcacgt
     8521 gagcatgagc atgggggcgg cgctcaaggc cgaccgcgcg ctcgacctcg cgcgccacgt
     8581 cctcgccgtg gagctgctct gcgcctgcca ggcgatcgag atgcgcgcgc c
//
LOCUS       DAOPQX010000138         8628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9146_length_8628_cov_4.910766, whole genome shotgun sequence.
ACCESSION   DAOPQX010000138 DAOPQX010000000
VERSION     DAOPQX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9146_length_8628_cov_4.910766"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..999
                     /locus_tag="PKK95_08850"
     CDS             49..999
                     /locus_tag="PKK95_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18362.1"
                     /translation="MSEVALSFENVVKRFGTTAAVSGVSLEVRRGEMFGLIGPDGAGK
                     TTTIRLACGLLAPAEGRVRLLGRDPVREHDEVTRSTGYLSQRFSLYGDLTIDENIAFF
                     AEIHGLTRYRERAERLLEMTQLTPFRSRLADRLSGGMKQKLALACTLVHEPELILLDE
                     PTTGVDPVSRREFWRLLSEFLAQGITIVMATPYLDEAERCARVALLSEGRVLVADSPD
                     TLREQLPGLVIEVLASPQKPAMEALAGVAGVIDVQNFGERIHARLGSWVGDQGSGIGD
                     HESVRVIESALRAAGVEVVSVRPVPASLEDVFITRVSDSG"
     gene            1204..2541
                     /locus_tag="PKK95_08855"
     CDS             1204..2541
                     /locus_tag="PKK95_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC18363.1"
                     /translation="MRGSRLVAALAVAATCGAPAIAAAQVQPQPEAGLTLEQAIERGL
                     AASHRLAEIEARRAGAEAVVAGSRAADRPQASLQAGYTRTNHVDEFGVQVPGQPPRII
                     YPDIPDNFRTRLDLQWPIFTFGRTDALERAARAEVEAAGFDLEAARSDLKLEITRAFW
                     AVVTAEESVRVVGESLKRMDAALADVRHRLEVGLVPPSDVLSVEAQRSRQQLLLVRAR
                     NHLDQARADLRRLTGLPPEAPLRALAPFEPPAPVEADPATLVREAEGARPDRRALAAR
                     VAGAGERQEAARANRRPTVALAGGVDYARPNPRIFPRMREWNESWDASVQFNWTFWDA
                     GRTKAAVAEASAAGRAAAERLREFDRLLALEVRQRQLDLASAAAAIAAANDGVRSAAE
                     ARRVVGDRYASGVATSTEVLDAHVALLQAELDRTEAIAGARLAEARLARALGR"
     gene            2620..3549
                     /locus_tag="PKK95_08860"
     CDS             2620..3549
                     /locus_tag="PKK95_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18364.1"
                     /translation="MSAIVVRDLTRRFGKFVAVDHVSFDVREGEIFGFLGSNGAGKST
                     TIRMLCGLLRPTSGTATVGGADVGTDPEGVKTRIGYMSQRFSLYEKLTVDQNIRFYAG
                     LYGISGERFDQRRAFVLEMAGLRGREDMFARDLPGGWRQRLALGCAILHEPPILFLDE
                     PTGGVDPVSRREFWRLIDRLSGSGVTVLVTTHYLDEAEYCHRIAIIHAGRLAALGSSR
                     ELKQLFASRPIVEIHTSRAVEAMRALEGMPEVEKSSLFGTAVHAVLSGPEVGPETVSA
                     WLGRHGHEVSAIDRVQPSLEDVFLDVVEKAGRA"
     gene            3546..4664
                     /locus_tag="PKK95_08865"
     CDS             3546..4664
                     /locus_tag="PKK95_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18365.1"
                     /translation="MRKAIAVGRKEFRQIARDRRTLAIMLLVPVGFLLLFGYALNFDV
                     KNVSMAVQDRDNSSESRALVSAFLNSGYFRFAGTVHGDQELHRLMDRGRAILVLVIPE
                     NTGRAIRRGEVAPVQVILDGLDSTTGVAVLGYSLSVVRSVSNRLLLEQGAPVPGVPPV
                     AVEPRIWYNPELRSALFLVPGLIGFIGMITAAVSTALSVVREKERGTWEQVRMAPINT
                     ISYIVGKTIPYLGLSIVSSMGIVLAAMVLFDLPMRGSWLALLLATTLFLLAALGMGLL
                     VSTIAESQLVAFQIVLILAFLPTFLLSGFIFPIQNMPVVIQAITYAVPARYFLVALRG
                     IVLKGVGLGAFWVQLAALVAFAVVVLGLASARLARERG"
     gene            4666..5787
                     /locus_tag="PKK95_08870"
     CDS             4666..5787
                     /locus_tag="PKK95_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18366.1"
                     /translation="MQRLLVLIWKELLELRQDPRMLPIVFIAPILQLTVLGYAISTDV
                     RNVPTVVVDWDRSTESRRLVEAFDASPYFTIKYTADSVEEASRWLETGAAWMVLTVPA
                     GYGRDVRTGHPAVVQVVADGTDANSASLALSYTSNLVAAYAQEVAAARAPSGVRAPGG
                     LRADVRIWFNPELSSRHFLIPGVLALLLIVMTVVLASMGIVREKELGTLEQLNVTPLR
                     RWELIVGKLLPYGLIGVIDIFLVVAVATLWFEVPLRGSFVLLFLMSLLFLVNTLGLGL
                     FVSTISNNQQQAMMSAVFFFVVPLMYLSGFVFPIDNMPAVIQPIAYAIPLTYYLVILR
                     GIFLKGVGIATLWPQALGLAVWGATILALATWRSRKTAG"
     gene            complement(5872..6315)
                     /gene="mscL"
                     /locus_tag="PKK95_08875"
     CDS             complement(5872..6315)
                     /gene="mscL"
                     /locus_tag="PKK95_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOC18367.1"
                     /translation="MLKEFKEFAMKGNVLDMAVGIIIGAAFGRIVSSFVSDVLTPPLG
                     LLLGGFDFSNLFINLSSGGYATIEAAKAAGAPTLNYGLFLQATIDFLIVAFAIFLLVK
                     WVNRMRPAAPAAAPATKECPMCTTAIPIKARRCPACTADLQGIGA"
     gene            complement(6424..7767)
                     /locus_tag="PKK95_08880"
     CDS             complement(6424..7767)
                     /locus_tag="PKK95_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC18368.1"
                     /translation="MKRNLLRIALAAGAAAALTLPGAADAPRTYAIVGGRIVTAAGPV
                     IENGTVVLRNGIVEAVGASVSVPPEAATIDGKGLSVYPGLIDMGTSAGLSAPATEAPR
                     NARTRMEVERARRRMLLRAHLDAASLLKADAPEFDRFAAAGITSVLLTPSGSGIRGRS
                     ALVNVAAGDDPPQIGQVANERAGMFVVKAPVALHLSYFGREGFDAYPTSLMGAIAFVR
                     QAFHDAAAYREETARQGKPGAGPPPAYDPALEALQPALAGSMPVAIEAASAVQIRRAL
                     ALAREFKLSPIVVGALGASEVVPDLKAAGAAVIYSLDYPVRPRSLAPGADEPVRVLRE
                     RANAPKVPAALDRAGVRFAFASSGLREPADFVKNASKAVAAGLPADAAVRALTIEAAR
                     IAGVAESLGSIEKGKRANLIVTEGDLFGAEAKVRHVFVDGRPLALRRSAGVPAAQ"
     gene            complement(7808..>8628)
                     /locus_tag="PKK95_08885"
     CDS             complement(7808..>8628)
                     /locus_tag="PKK95_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC18369.1"
                     /translation="WEESTLKPIAGISFQFPGVGARRGSYGAPPAPERTYEELRKERD
                     AKLDDLLRLFDQARAYAAAGEGRERDWVLEALVPVVQRRIPLYTSALGEPAIREAVAF
                     AERANVRIVIASGPEAALAAPLLKEKGIPVVLGPVLQLPPREDQYHAFSYQAAGELAR
                     AGVRFAFATNDNADVRQLPWHAAISVAWGLPREQALRALTIDAAEILGVSDQVGSLEP
                     GKVANLFVAKGDPLEARTEITHVVINGREVDLDNKHLSLYERYLKRQSPLQVGH"
BASE COUNT         1184 a         3011 c         3092 g         1341 t
ORIGIN      
        1 gggggggggg gttgggggcg gggggctagg ggccaggggc caggaagcat gtccgaggtt
       61 gcgctgtcgt tcgagaacgt cgtgaagcgg ttcgggacga cggccgcggt gagcggagtg
      121 tcgctcgagg tgcgccgcgg cgagatgttc gggctgatcg gcccggacgg cgcgggcaag
      181 accacgacga tccgcctcgc ctgcgggttg ctcgcgccgg cggaagggcg ggtgcggctg
      241 ctcggccgcg acccggtgcg cgagcacgac gaggtcaccc ggtccacggg ctacctgtcg
      301 cagcgcttca gcctgtatgg cgacctcacc atcgacgaga acatcgcgtt cttcgctgag
      361 attcacggcc tcacgcgcta tcgcgagcgc gccgagcggc tgctcgagat gacgcagttg
      421 acgcccttcc gcagccggct cgccgaccgg ctctcggggg gaatgaagca gaagctggcg
      481 ctggcctgca cgctcgtgca cgagccggag ctgatcctcc tcgacgagcc gacgaccgga
      541 gtcgatccgg tgtcgcggcg cgagttctgg aggcttctgt cggagttcct cgcacagggc
      601 atcacgatcg tcatggccac gccgtacctg gacgaggcgg agcgctgcgc gcgggtggcg
      661 ctgctctcgg agggccgcgt cctggtggcc gactcgcccg acaccctgcg cgagcagctt
      721 ccaggcctcg tcatcgaggt attggcctcg ccccagaaac cggccatgga ggcactggcg
      781 ggcgttgccg gcgtgatcga cgtccagaac ttcggcgagc ggattcacgc gcgcctcggg
      841 tcatgggtcg gggatcaggg atcggggatc ggggaccatg aatcggtgcg cgtgatcgag
      901 agcgcactgc gcgcggcggg cgtcgaggtg gtgagcgtgc ggcccgtgcc cgcctcgctc
      961 gaggacgtgt tcatcacgcg cgtaagcgac tcaggctgaa cgggagttca cggttctgaa
     1021 ccacaaagga cagaaaggac acgaagaggt gcccgtgtga ggaggagcag cacgagtcgc
     1081 tcgtcgatgc gccggccctc ggcggccgcg cagcggcccg ccacgcgggc ggctacaggg
     1141 aagcttcttt gtgttctttg tgatccttgt ggtgaatccg taatcgtgct tcgggagatg
     1201 agcatgagag gttcgagact tgtcgcggcc ctcgcggtgg ctgctacgtg cggcgccccg
     1261 gcgattgcgg cggcgcaggt gcaaccgcag cccgaggccg ggttgacgct cgagcaggcc
     1321 atcgagcgcg ggcttgccgc cagccaccgc ctcgcggaga tcgaggcccg ccgcgcgggc
     1381 gccgaggcgg tcgtggccgg cagccgcgct gccgaccgcc cccaggcctc cctgcaggcc
     1441 ggctacaccc gcaccaacca cgtcgacgag ttcggcgtcc aggtaccggg ccagccgccg
     1501 cgcatcatct acccggacat ccccgacaac ttccgcacgc gcctcgacct gcagtggccg
     1561 atcttcacgt tcggccgcac cgacgcgctc gagcgggccg cgcgggccga ggtggaggcg
     1621 gcggggttcg acctcgaggc ggcacgaagc gacctgaagc tcgagatcac gcgcgccttc
     1681 tgggcggtgg tcacggcgga ggagtcggtg cgcgtggtgg gcgaatcgct gaagcgcatg
     1741 gatgctgcgc tcgccgacgt ccgccatcgc ctcgaggtgg gcctggtccc gccaagcgac
     1801 gtgctgtcgg tggaggcgca gcggtcgcgc cagcagctcc tgctcgtgcg ggcgcggaac
     1861 cacctcgacc aggcgcgcgc cgacctgcgc cgcctgaccg gcctgccccc cgaggcgccg
     1921 ctgcgagcgc tggcgccgtt cgagccgccc gcgcccgtcg aggccgaccc cgcgaccctc
     1981 gtccgcgagg cggagggcgc ccgccccgat cgccgcgcgc ttgccgcgcg cgtggccggc
     2041 gccggggagc gccaggaggc cgcgcgcgcc aaccggcgcc cgaccgtggc gctggccggc
     2101 ggcgtcgatt acgcgcgccc gaacccgagg atcttccccc gcatgcgcga gtggaacgag
     2161 tcctgggacg cgagcgtgca gttcaactgg accttctggg acgcgggccg cacgaaggcg
     2221 gccgtggcgg aggcgagcgc cgccgggcgt gcggccgcgg aacggctgcg ggagttcgac
     2281 cgcctcctcg ccctcgaggt gcgtcagcgc cagctggacc tcgcgtcggc cgcggccgcg
     2341 atcgccgccg ccaacgacgg cgtccgcagc gccgccgagg cccgccgcgt cgtcggcgac
     2401 cgctacgcct cgggcgtggc gaccagcacc gaggtgctcg acgcgcacgt tgcgctgctc
     2461 caggccgagc tcgaccgcac cgaggcgatc gccggcgccc ggctcgccga agcccgcctc
     2521 gcgcgggcgc tcgggcgatg actgaggaca gagggctggg gaccgggagg cggccgtcga
     2581 gtggtgtgcg attgaccggg attgggattg ggattcgtga tgagcgcgat cgtcgtccgt
     2641 gacctcacgc gccggttcgg gaaattcgtg gccgtggacc acgtgtcgtt cgacgtccgc
     2701 gagggggaga tcttcggctt cctcggcagc aacggcgccg gcaagtcgac cacgatccgg
     2761 atgctgtgcg gcctgctgcg cccgacgtcg ggcaccgcga ccgtcggcgg ggccgacgtc
     2821 gggaccgatc ccgaaggcgt caagaccagg atcggctaca tgtcgcagcg cttctccctg
     2881 tacgagaagc tgacggtgga tcagaacatc cgcttctacg ccggcctcta cggcatctcg
     2941 ggcgagcgct tcgatcagcg gcgcgccttc gtgctcgaga tggccggcct gcgcggccgc
     3001 gaggacatgt tcgcgcgcga cctccccggc gggtggcggc agcggctcgc cctcggctgc
     3061 gcgatcctcc acgagccgcc gatcctcttc ctcgacgagc cgaccggcgg cgtggacccg
     3121 gtgtcgcggc gcgagttctg gcgcctgatc gatcggctct ccggctcggg cgtcaccgtc
     3181 ctggtgacaa cccactacct ggacgaggcc gagtactgcc accggatcgc gatcatccac
     3241 gcgggcagac tggcggcgct cggctcttcg agggagttga agcagctgtt cgcgtcgcgc
     3301 ccgatcgtgg aaatccacac ttcgcgggcg gtcgaggcca tgcgggcgct ggagggcatg
     3361 ccggaggtcg agaagagcag cctgttcggg acggcggttc acgccgtgct gagcgggccg
     3421 gaggtcggcc cggagaccgt ctccgcgtgg ctcgggcggc acggtcacga ggtgtcggcg
     3481 atcgaccgtg tccaaccctc gctcgaggac gtgttcctcg acgtggtgga gaaggcgggg
     3541 cgcgcatgag gaaggcgata gcggtcggac ggaaggagtt ccggcagatc gcgcgcgacc
     3601 ggcgcacgct cgccatcatg ctgctcgtgc cggtcggctt cctcctgctg ttcggctacg
     3661 cactcaactt cgacgtgaag aacgtgagca tggccgtgca ggaccgggac aacagctcgg
     3721 agagccgggc gctcgtctcg gcgttcctga actcgggcta cttcaggttc gccggcacgg
     3781 tgcatgggga ccaggagctg caccgcctga tggaccgggg ccgcgccatt ttggtgctcg
     3841 tcatccccga gaacacaggg cgtgcaatcc gccgaggcga ggtggcgccc gtacaggtca
     3901 tcctcgacgg cctcgactcg acgacgggcg ttgccgtcct gggctattcg ctctcggtcg
     3961 ttcggagcgt ctcgaaccgg ctgctgctcg agcagggcgc cccggttccc ggggtcccgc
     4021 ccgtggccgt cgagccgcgc atctggtaca accccgagct gcgcagcgcc ctgttcctgg
     4081 tgcccgggct gattggcttc atcgggatga tcacggccgc cgtctcgacc gcgctgtcgg
     4141 tggtgcggga gaaggagcgg ggcacctggg agcaggtgcg catggccccg atcaacacca
     4201 tctcctacat cgtcggcaag acgattccgt acctcgggct gtcgatcgtc tcgtcgatgg
     4261 gcatcgtcct ggccgcgatg gtgctgttcg acctgccgat gcgggggtcg tggctggcgc
     4321 tcctgcttgc gacgacgctg ttcctgctgg ccgcgctcgg gatggggctg ctcgtctcga
     4381 ccatcgccga gtcgcagctg gtggcgttcc agatcgtgct gatcctcgcg ttcctgccga
     4441 ccttcctgct gtcgggcttc atcttcccga tccagaacat gccggtggtc atccaggcga
     4501 tcacctacgc cgtgcccgcg cggtacttcc tggtggcctt gcgggggatc gtgctgaagg
     4561 gcgtcggcct cggggcgttc tgggtgcagc tggcggcgct ggtggcgttc gcggtggtgg
     4621 tcctgggact ggcctcggcg cgcctggcgc gggagcgagg gtgagatgca gcggctgctg
     4681 gtgctgatct ggaaggaact gctcgagctg cggcaggacc cgcgcatgct gccgatcgtg
     4741 ttcatcgcgc cgatcctgca gctcaccgtg ctcggctacg cgatcagcac cgacgtcagg
     4801 aacgtgccga ccgtcgtggt ggactgggac cggagcaccg agtcgcgccg cctggtcgag
     4861 gcgttcgacg cctcgccgta ctttacgatc aagtacacgg cggacagcgt ggaggaggcg
     4921 tcgcggtggc tcgagaccgg cgccgcctgg atggtgctca cggttccggc cgggtacggg
     4981 cgcgacgtca ggacggggca cccggccgtc gtccaggtgg ttgccgacgg gaccgacgcg
     5041 aactcggcct cgctcgccct cagctacacg agcaacctgg tcgcggccta cgcccaggag
     5101 gtcgcggcgg ctcgggcccc ttcaggcgtc cgggcgccgg gcggcctgcg cgccgacgtg
     5161 cggatctggt tcaaccccga gctgtcgagc aggcacttcc tgatcccggg cgtcctcgcc
     5221 ctgctgctga tcgtgatgac ggtagtgctt gcctcgatgg gaatcgtccg cgagaaggag
     5281 ctggggacgc tcgagcagct caacgtgacc ccgctcaggc gctgggagct gatcgtcggg
     5341 aagctgctgc cctacggcct catcggcgtc atcgacatct tcctggtggt cgcggtggca
     5401 accctgtggt tcgaggtgcc gctgcgtggc agcttcgtgc tgctgttcct gatgtcgctg
     5461 ctgttcctgg tgaacacgct gggcctcggc ctgttcgtct cgacgatctc gaacaaccag
     5521 cagcaggcga tgatgtcggc cgtcttcttc ttcgtcgtgc cgctgatgta cctctcgggc
     5581 ttcgtcttcc cgatcgacaa catgcccgcg gtgatccagc cgatcgccta cgccatcccc
     5641 ctgacctact acctggtgat cctgaggggc atcttcctca agggagtggg gatcgccacg
     5701 ctgtggccgc aggcgctcgg cctggccgtc tggggcgcga caatcctcgc gctggccacg
     5761 tggcggtcgc gaaagaccgc ggggtagggt tgccgcgccc gcgcctcggc aacccggccc
     5821 ttgtccgcct tcagccccgg ccctccgcct tcagccccgg ccctccgcct tctacgcccc
     5881 gatcccctgc aggtcggcag tgcacgccgg gcagcgccgg gccttgatcg ggatggcggt
     5941 cgtgcacatc gggcactcct tggtggcagg cgcggcggca ggcgcggccg gccgcatcct
     6001 gttcacccac ttcacgagca ggaagatcgc gaatgcaacg atcaggaagt cgatcgtcgc
     6061 ctgcaggaac aggccgtagt tcagggtcgg cgcgcctgcc gccttggccg cctcgatggt
     6121 ggcgtagccg ccgctcgaca ggttgatgaa caggttgctg aagtcgaagc cgccgagcag
     6181 aaggcccagc ggaggcgtca ggacgtcgga aacgaacgag gagacgatcc tgccgaacgc
     6241 ggcgccgatg atgatgccca cggccatgtc gagcacgttg cccttcatgg cgaattcctt
     6301 gaactccttg agcatggatt ctcctcccgt caacgggtga ccggcagcgt gtggttcgag
     6361 gatgccgcag tatacgcccg ttccaggcgg gcggcgcccc cgccgccggc agagacgccg
     6421 gcgctactgc gccgcaggga cgccggcgct gcggcgcagc gccagcggcc tgccgtccac
     6481 gaacacgtgg cggaccttcg cctcagcgcc gaacaggtcg ccttccgtca cgatcaggtt
     6541 cgcccgcttg cccttctcga tcgacccgag gctttcggcc acgcccgcga tccgcgcggc
     6601 ctcgatcgtc agcgcccgca ccgcggcgtc ggctggcagg ccggcggcca ccgccttcga
     6661 cgcgttcttg acgaagtcgg cgggctcgcg cagcccgctc gaggcgaacg cgaacctcac
     6721 gccggcccgg tcgagcgcgg cgggaacctt gggggcgttg gcccgctcgc gcagcacgcg
     6781 caccggctcg tcggccccgg gtgcgaggct gcgcggccgc accgggtagt cgaggctgta
     6841 gatcaccgcc gcacccgcgg ccttcaggtc gggaaccacc tcggaagccc cgagcgcccc
     6901 gacgacgatg ggagacagct tgaactcgcg ggccagggcc agggcgcggc ggatttgaac
     6961 ggcgctcgct gcctcgatcg cgaccggcat cgatccggcc agcgcgggct gcagcgcttc
     7021 cagcgcgggg tcgtacgcgg ggggcggacc cgcgcccggc ttcccctgcc gtgccgtctc
     7081 ctcccgatac gcggctgcat cgtggaatgc ctggcggacg aaggcgattg cccccatcag
     7141 ggaggtgggg taggcatcga acccctcgcg gccgaagtac gacaggtgca gggcgacagg
     7201 cgccttcacc acgaacatcc cggcccgctc gttggcaacc tggccgatct gcggcgggtc
     7261 gtcaccggcg gcgacgttca ccagggcgct gcgtccgcgg atcccggaac ccgagggcgt
     7321 caggagcacc gaggtgatgc cggcggccgc aaagcgatcg aactcgggag cgtcggcctt
     7381 caggagcgag gccgcgtcca ggtgcgcccg cagcagcatg cggcgccggg cgcgctcgac
     7441 ctccatccgc gtccgggcgt tgcgcggcgc ttcggtagcc ggcgccgaca gccccgccga
     7501 ggttcccatg tcgatgaggc cggggtacac gctcagcccc ttgccgtcga tggtggccgc
     7561 ctcgggcggc acgctcaccg aggcgccaac cgcttcgacg atgccgttgc gaagcaccac
     7621 cgtcccgttc tcgattaccg ggcccgcggc ggtgacgatg cgcccgccga cgatggcata
     7681 ggtgcgcggc gcgtcggcgg cgccaggcag cgtgagggcg gccgcagcac cggccgccaa
     7741 ggcgattcgc aggaggttcc gtttcatcgc aagtccccgg ggccaggccc cacttctccc
     7801 gtcagtctca atggccaacc tgcaggggcg actgccgctt caggtaccgc tcgtacagcg
     7861 acaggtgctt gttgtcgaga tccacctcgc ggccgttgat caccacgtgc gtgatctcgg
     7921 tgcgcgcctc cagcgggtcc cccttggcga caaacaggtt cgccaccttc cccggctcca
     7981 ggctcccgac ctggtccgac acgccgagga tctcagctgc atcgatcgtc agcgcccgca
     8041 gcgcctgctc gcgcggcagc ccccacgcca ccgagattgc cgcatgccac ggcagctgcc
     8101 gcacgtctgc gttgtcgttc gtggcaaacg cgaaccggac gcccgctcgc gccagctccc
     8161 ccgccgcctg gtagctgaac gcgtggtact ggtcttcgcg cgggggcagc tgcagcaccg
     8221 ggcccaggac gaccgggatg cccttctcct tcagcagcgg cgccgccagc gcggcctcgg
     8281 gtcccgacgc gatcacgatc cgcacgttcg cgcgctccgc gaacgccacg gcctcgcgaa
     8341 tggccggttc gcccagggcg ctggtgtaca gcgggatccg gcgctgcacc accggcacca
     8401 gcgcctcgag cacccagtcg cgctcgcgcc cctcccccgc cgcggcgtac gcgcgcgcct
     8461 ggtcgaagag gcgcagcagg tcgtcgagct tcgcgtcccg ctccttgcgc aactcctcgt
     8521 aggtgcgctc cggggcgggc ggcgcgccgt acgacccgcg ccttgctccg acgccaggaa
     8581 actggaagct gatgcccgcg atgggcttca gtgtgctctc ctcccagg
//
LOCUS       DAOPQX010000139         8620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9161_length_8620_cov_4.444600, whole genome shotgun sequence.
ACCESSION   DAOPQX010000139 DAOPQX010000000
VERSION     DAOPQX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9161_length_8620_cov_4.444600"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1082)
                     /locus_tag="PKK95_08890"
     CDS             complement(24..1082)
                     /locus_tag="PKK95_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18370.1"
                     /translation="MRRLFDRIRWSLSGDRDLDEEIRAHLEIEASHLEAEGLTPADAR
                     AAARRRFGSPTLARENSREVWTFAPLETWLQDVRFALRLLARGPLFTLTAVVSLALGI
                     GAAVAAFSLLDTVLLRPLPVTDPQQLVLLRWVSGARGDLLAESINGWLGLDGDGQRTS
                     TSFSYSAYKDLAEHQSSLTGLVAFAEIPDLLVGTGAEADVSTGQYVSGSYFQALGLRP
                     AAGRLLDPADDRRDVQAVAVISYAGWRSRFGGSPSAIGARVLLNRVPVTIVGVMPRAF
                     RGCLQIGSDPEFTNGRGGGLGPGHGPRPDGRGEPPGESRVVRGEADRSLDADCWNVRP
                     RRPGAGRGLPAGAPRIEG"
     gene            complement(1154..1504)
                     /locus_tag="PKK95_08895"
     CDS             complement(1154..1504)
                     /locus_tag="PKK95_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC18371.1"
                     /translation="MSDEERLTLLQGTLDMLILRTLRWGPAHGQAIARFIQDTSGDAL
                     LVEHGSLYPALQRLQQKRWIVAEWGASDNNRRAKYYRLTPAGRRQLAREHSNWERLVE
                     AIGRIMHPAAQGKP"
     gene            complement(1667..2575)
                     /gene="nhaR"
                     /locus_tag="PKK95_08900"
     CDS             complement(1667..2575)
                     /gene="nhaR"
                     /locus_tag="PKK95_08900"
                     /inference="COORDINATES: protein motif:HMM:NF008284.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HOC18372.1"
                     /translation="MSADFPSFWLNYHHLLYFWTVVREGGVVRAAERLRLSQPTVSSQ
                     VRALEKALGERLFIRTGRRLVPTDVGQVVYRYADEIFSLGRELVDTVHGRGEGRPARL
                     VVGVADALSKLIAYRLVAPVLALPGPIRVVCREGSTEKLLAQLALHEVDVVLSDTPVP
                     PTVRVRAYNHLLGECGLTFFAAPAAARRLGRGFPGSLAGQPFLLPGEGSVLRRQLEDW
                     LQQAGIRPAVVGEFDDLALMKVFGENGAGAFAAASAIEPEIRRQYRVVPIGRIESVRQ
                     QFYAISAERRLKHPAVSVIVERGREL"
     gene            2737..3732
                     /locus_tag="PKK95_08905"
     CDS             2737..3732
                     /locus_tag="PKK95_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOC18373.1"
                     /translation="MDLLFPFAQYWWFYAAFTAGVLVLLAIDLGVFHRHAHVVSFKES
                     LTWSAVWVALALAFNYGLFLYAQSRFPGEVATRVALEFLTGYLVEKSLAVDNVFVFVL
                     VFSYFAVPLKYQHRVLFYGIIGALVFRAGFIALGSVLMQHKAVVIFFGVVLIVSGIKM
                     MGGPRKSIEPGKNPLVRLFRRLVRVTEEAHGPSFFVRLAGRWHATPLLVALLFLEASD
                     IVFAVDSVPAIFAITREPMLVFTSNVFAILGLRAMYFLLAGAIERFHLLNYGLALILV
                     FVGLKMVWLNEAFGGKFPIEWSLGIIAALLAGAIVASLARPRPAEEAPGLEAQDS"
     gene            complement(3846..5012)
                     /locus_tag="PKK95_08910"
     CDS             complement(3846..5012)
                     /locus_tag="PKK95_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC18374.1"
                     /translation="MRNGLLLLVPLVLAATAPRAGFEPSPAVPTTLSSARSAAAAVQD
                     ATRLTADQVMRMPKIDAHAHLGAVDSADRDAFVRFLERIDLRWLTIATGGMNAARLDH
                     QIESMEGYHRAAPARIGWATSFHLSGWGGAGWADAARKTLSDGFAKGAVAVKIWKDVG
                     MELKDAGGQFVMADDRRLDPVYQAAAAAKKPLVAHLGEPRNCWLPVEEMTVQSDRNYF
                     SRNPQYHGFLRPDIPSYEKQVAARDAVLERHPGLRVIGCHLASLEYDVDEVAKRLERF
                     PNLAVDLAARIVHFQVQPREKVRAFILRYQDRILYGTDFTFGRRPGAPLDVEQALASL
                     EANYRLDAEYFATDAEVEVPRVRPGYKTRGLALPAPVLRKIYFENARAWYPGIQ"
     gene            complement(5046..6071)
                     /locus_tag="PKK95_08915"
     CDS             complement(5046..6071)
                     /locus_tag="PKK95_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18375.1"
                     /translation="MHRRDFLLASGTALASLFGRTATAFSLPPVSGRLMTVLGPIEPE
                     AFGSALCHEHVLVDFIGADTITPERYDANEVYQVALPYLRRAHELGCRGLVECTPAYL
                     GRDVRLLQRLSRESGLHLLTNTGYYGAAKDQYVPRHAYDESADDLASRWAAEWENGIE
                     GTGIRPGFIKTGVDAGPLSAIDRKLIQAAARTHARTGLAIASHTGDTRAALEQIEVLA
                     SERVRHDAFIWVHAQNDWQAESRIEAARKGVWIEIDNVAPPSVAECVARAVELKARGL
                     LGRLLVSHDAGWYSPGEPHGGQFRGFDAIYTALVPALRRAGFANDEVRRLLVSNPAEA
                     FAVRPVR"
     gene            complement(6176..6679)
                     /locus_tag="PKK95_08920"
     CDS             complement(6176..6679)
                     /locus_tag="PKK95_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18376.1"
                     /translation="MMKVLGAVALAAVLALPVAAQQSQPAQQSQPTQQAPHSQKQVGT
                     AGAMSTVEAIVDNPEQFYNQTVTIAGEVNEVHGKRAFDIREKGVLDVDDKLLILSKKD
                     LANVDKDSMVEVRGKVRPFVKDDIAREHNITDWSDYGLDADFFSSYENRPVLIAESVQ
                     VRAPGQK"
     gene            complement(6905..7675)
                     /locus_tag="PKK95_08925"
     CDS             complement(6905..7675)
                     /locus_tag="PKK95_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18377.1"
                     /translation="MTSSAERASRSAIHALPLAALAAGLMWFAPAPVVQAQEFENRTI
                     FTFDSPVQVPGAVLPPGRYVFQLIDPGQPARSVEIRKPDGGPALATTVTLPIRRPESE
                     DASGVKVSFVGTRGDQPPAIKSWFYPGEETGHLFVYPEKQARQIAEATEQYVLASEEA
                     EASGTVFLVGADGMRQQYSGEAQRAYLAGSDDRRAAGRADAEERLDRIARTVQTLLEG
                     REVGTSGSPSEAVSISRDDLQAIQRHVEQLRRQLEQKR"
     gene            complement(7842..>8620)
                     /locus_tag="PKK95_08930"
     CDS             complement(7842..>8620)
                     /locus_tag="PKK95_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18378.1"
                     /translation="SWPSPEFCVRAIEWMEIQAIRTRSRGADDTLIDRILARASGEAA
                     AEEQAGRAVEAYRRYSALAAMMAGLRDVAPFERKARELRALPEVRSAVKEEADSMPRE
                     KAAKERFARVVIDALEGEDRTLAMQELLRTVEEMRAQAERPRRDAGRMAARRVLVSSW
                     SWMNEENARDIEAGRPARAAERLSLMARIRPADAAVDYQRARALALDGKKGAAIDALR
                     SAVKKGFDDGAAIDAEPAFAPLRQEREFQEIVERLRRHGA"
BASE COUNT         1296 a         2973 c         2971 g         1380 t
ORIGIN      
        1 cgcgccgcag cgcgaccatg ggatcaaccc tcgatgcgcg gcgcgccggc aggtagcccg
       61 cggccagcgc cagggcggcg agggcgaacg ttccagcaat cagcgtcgag ggatcggtcg
      121 gcttcacccc gaacaactcg gctctcacca ggcggctcgc cgcgaccgtc agggcgaggc
      181 ccgtgcccag gccccaggcc gccaccacgg ccgttggtga actcgggatc gctgccgatc
      241 tgcaggcagc cgcgaaaagc ccggggcatc acgccgacga tggtcacggg gacccggttc
      301 agcaggaccc gggcgccaat cgcggacggc gagccgccga accgcgaccg ccacccggcg
      361 tagctgatca cggccaccgc ctggacatcg cgccgatcgt cggcggggtc cagcagccga
      421 cctgcggccg gccggaggcc cagtgcctgg aagtaggacc ccgacacgta ctgcccggtg
      481 ctgacgtctg cttcggcacc ggttccgacg aggaggtcgg ggatctcggc gaaggcgacc
      541 aggccggtca gcgatgactg gtgctcggcg aggtccttgt acgctgagta cgagaacgag
      601 gtggaggttc gctggccgtc gccgtccagg ccgagccagc cgttgatcga ttcggccaac
      661 aggtcgccgc gcgctcccga cacccagcgg agcaggacga gctgctgcgg gtcggtcacc
      721 gggaggggcc gcagcagcac cgtgtcgagc aggctgaatg cggcgaccgc ggccccgatg
      781 cccagcgcaa gcgagacgac agcggtcagg gtgaaaagcg ggccccgggc gagaagacgc
      841 agggcaaagc ggacgtcctg gagccaggtc tcgagcggcg cgaaggtcca gacctcccgg
      901 ctgttctccc tggcgagggt ggggctgccg aagcggcggc gggccgcggc cctggcgtcg
      961 gccggcgtca gtccctcggc ctcgagatgc gacgcctcta tctcgaggtg cgcccggatc
     1021 tcctcgtcga ggtcgcggtc gcccgagagg ctccagcgga tccgatcgaa caggcgtcgc
     1081 atggcatcgg ccccttcagc gaaaacggca ggcggaggcg cgcgtgagtc gggcgaagtc
     1141 cggggcgtcg gcgctacggc ttgccctgcg cggcagggtg catgatccgg ccgatggctt
     1201 cgaccagccg ctcccagttc gagtgctcgc gggcgagctg acggcgaccg gcgggtgtga
     1261 gcctgtagta cttcgcgcgg cggttgttgt ccgaggcgcc ccactcggcc acgatccagc
     1321 gcttctgctg caggcgctgc aacgcggggt acagcgagcc gtgctcgacc aggagcgcgt
     1381 cgccggaggt gtcctggatg aagcgcgcga tcgcctggcc gtgcgccggt ccccagcgca
     1441 gcgtgcgcag gatcagcatg tcgagcgtgc cctgcaggag cgtgaggcgt tcctcgtccg
     1501 acatgggtag acactctacc ctaagtcggg tcgaatgtct accagaagat ggctcacggc
     1561 tcacggctca aggctcacgg ctcaaggcac agggctcaag gctcacggaa ggctcacggc
     1621 tcagggctca aggctcaagg ttcacgactc gaggctcagg gcgcgctcag agctcccgtc
     1681 cccgttccac gatcaccgac acggcggggt gcttgagccg ccgctcggcc gagatggcgt
     1741 agaactgctg gcggaccgat tcgatccgcc cgattggcac cacccggtac tgccgccgga
     1801 tttccggctc gattgccgac gcggccgcga acgccccggc accgttctcg ccgaacacct
     1861 tcatcagcgc gagatcgtcg aactcgccca cgaccgccgg cctgatgccc gcctgctgca
     1921 gccagtcctc gagctggcgc ctcaggacgc tgccctcgcc gggcaggaga aacggctgcc
     1981 ctgccagcga gccggggaag ccgcgtccga gccggcgcgc cgccgcgggt gccgcgaaaa
     2041 aggtcagccc gcactcgccg agcaggtggt tgtaggcgcg aacccggacg gtgggcggga
     2101 cgggcgtgtc cgacaggacg acgtccactt cgtggagggc gagctgggcc agcagcttct
     2161 cggttgaacc ctcccggcac accacgcgga tgggcccggg aagggccagc accggggcga
     2221 ccagccgata ggcgatgagc ttgctgagcg cgtcggccac gccgacgacc aggcgcgcgg
     2281 ggcggccttc gccccggccg tgcacggtgt ccaccagctc gcggcccagc gagaagatct
     2341 cgtcggcgta gcggtacacc acctggccga catcggtggg caccaggcgg cggcccgtgc
     2401 ggatgaacag ccgctcgccg agggccttct cgagggcgcg cacctggctg ctgacggtcg
     2461 gctgcgagag gcgcagccgc tcggcggccc ggacgacgcc cccctcgcgg acgacggtcc
     2521 agaagtagag caggtggtga tagttgagcc agaacgaagg gaagtcggcg ctcatggcag
     2581 gtgaaggctc ccgggtcgcg ccgcgcccgt ctcgcattcg cgcgcggcag agctcggcac
     2641 ggccgggcgg caggcccggc atacatagac aagagctatg atacagcaac aatctttgta
     2701 tttgtctatg ataccgccgc ccggtatcct cggcggatgg atctgctgtt ccccttcgcg
     2761 cagtactggt ggttctacgc cgcgttcacc gccggagtgc tcgtgctgct ggcgatcgac
     2821 ctcggcgtct tccaccggca cgcgcacgtc gtctccttca aggagtcgct gacctggtcg
     2881 gccgtgtggg tcgccctcgc gctcgccttc aactacgggc tcttcctcta cgcgcagtcg
     2941 agattccccg gcgaggtggc cacgcgcgtg gcgctcgagt tcctgacggg gtacctggtc
     3001 gagaagtcgc tcgccgtgga caacgtcttc gtcttcgtcc tcgtgttcag ctacttcgcg
     3061 gtgcccctga agtaccagca ccgggtcctg ttctacggga tcatcggcgc gctggtcttc
     3121 cgggcagggt tcatcgcgct cggctcggtg ctgatgcagc acaaggccgt cgtcatcttc
     3181 ttcggggtcg tgctcatcgt ctcgggcatc aagatgatgg gggggcccag gaagtcgatc
     3241 gagccgggga agaacccgct ggtccgcctg ttccggcgcc tcgtgagggt gaccgaggag
     3301 gcgcacggcc cgtcgttctt cgtccggctg gccggccggt ggcacgccac gccgctcctt
     3361 gtcgccctcc tgttcctcga ggcctccgac atcgtcttcg cggtcgactc ggtgccggcg
     3421 atcttcgcga ttacccgcga gccgatgctg gtgttcacct cgaacgtgtt cgccatcctc
     3481 ggcctgcggg cgatgtactt cctgctggca ggagccatcg agcgattcca cctgctcaac
     3541 tacggcctcg cgctgatcct cgtgttcgtc gggctgaaga tggtgtggct gaacgaggcg
     3601 ttcggcggga agttcccgat cgagtggtcg ctggggatca tcgcggcgct gctcgccggg
     3661 gcgatcgtcg cctcgctggc ccgcccgcga ccggcggagg aggcgcctgg actcgaggcg
     3721 caagactcgt agtcgtgacg cgaggcaggg cgggggcgag gggacaggcg atgacgccca
     3781 gaggacaggc gaggacgccc agaggacagg cgaggacgcc cagaggacag gcgaggacgc
     3841 ctgtcctact ggatgccggg gtaccaggcc cgggcgttct cgaagtagat cttccgcagc
     3901 accggtgcgg gcagcgcgag gccgcgggtc ttgtagccgg ggcgcacgcg aggcacctcg
     3961 acctcggcgt cggtggcgaa gtactcggcg tcgaggcggt agttggcctc gaggctggcc
     4021 agcgcctgct cgacgtccag cggcgctccc gggcggcgcc cgaaggtgaa gtcggtcccg
     4081 tagaggatcc ggtcctggta gcgaaggatg aacgcccgga ctttctcccg cggctgcacc
     4141 tggaagtgga cgattcgcgc ggccaggtcc accgcgaggt tcgggaacct ctcgagccgc
     4201 ttcgccacct cgtccacgtc gtactcgagg ctcgccaggt ggcagccgat gacccgcagc
     4261 cccgggtgcc gctcgaggac ggcgtcgcgg gccgccacct gcttctcgta ggacgggatg
     4321 tcggggcgca ggaagccgtg gtactgcggg ttgcgggaga agtagttgcg atcgctctgg
     4381 accgtcatct cctcgaccgg cagccagcag ttgcgaggct cgccgagatg cgcgacgagc
     4441 ggcttcttcg ccgcggcagc ggcctggtac acggggtcga ggcggcggtc gtcggccatg
     4501 acgaactgcc caccggcgtc cttcagctcc atgccgacgt ccttccagat cttcacggcc
     4561 acggcgccct ttgcgaaccc gtcggagagg gtcttgcgcg cggcgtcggc ccaccccgca
     4621 ccgccccacc cggacaggtg gaaggaagtt gcccagccga ttcgcgcggg cgccgcccga
     4681 tggtatccct ccatcgattc gatctggtga tcgagccgcg cagcgttcat gccgccggtc
     4741 gcgatggtca gccagcgcaa gtcgatccgc tcgaggaagc gcacgaaggc gtcgcgatcg
     4801 gcggaatcga cggcgccgag gtgcgcgtgc gcgtcgatct tcggcatcct catcacctga
     4861 tcggccgtga ggcgggttgc gtcctggacg gccgcggcgg ccgaccgcgc gctggaaagc
     4921 gtggtcggga cagcgggcga aggttcgaac cccgcgcgcg gggcagttgc cgcgagcacg
     4981 agcggtacga gcagcagcag gccgtttctc atggggcgga ttgttgctcc agaaggagcg
     5041 gcaggtcaac ggacgggccg caccgcgaag gcctccgccg gattggagac gagcagccgt
     5101 cgcacctcgt cgttcgcgaa gccggcgcgg cggagggcgg gcaccagcgc ggtgtagatc
     5161 gcgtcgaacc cgcgaaactg gccgccgtgc ggttccccgg ggctgtacca gcctgcgtcg
     5221 tgcgagacca ggagccggcc gagcaggccg cgcgccttca gctcgaccgc gcgcgccacg
     5281 cactcggcga ccgacggggg agcgacgttg tcgatctcga tccacactcc cttgcgcgcg
     5341 gcctcgatgc ggctctctgc ctgccagtcg ttctgcgcgt gaacccagat gaaggcgtcg
     5401 tggcgcaccc gctcggatgc caggacctcg atctgctcga gcgctgcgcg cgtgtcgccg
     5461 gtgtgagagg cgattgcgag gcccgtgcgg gcatgcgtgc gggctgccgc ctggatcaac
     5521 ttccggtcga tcgccgagag cgggccggcg tcgacgcccg tcttgatgaa cccggggcgg
     5581 atccccgtcc cctcgatccc gttctcccac tccgccgccc agcggctggc caggtcgtcg
     5641 gcgctctcgt cgtacgcatg acgcggcacg tactgatcct tggcggcgcc gtagtacccg
     5701 gtgttggtca ggaggtggag cccggactcc ctcgacaagc gctgcagcag ccggacgtcc
     5761 cgccccaggt acgcgggcgt gcactcgacc aggccgcggc atcccagctc gtgggcgcgc
     5821 cgaaggtagg gaagcgcgac ctggtagacc tcgttcgcgt cgtaccgttc aggggtgatc
     5881 gtgtcggcgc cgatgaagtc caccagcacg tgctcgtgac agagcgcgct gccgaacgcc
     5941 tcaggctcga tcggcccgag gacggtcatc agcctcccgg agaccggcgg cagcgagaac
     6001 gcggttgcgg ttcgaccgaa caggctggcg agggccgtgc cggaggcgag caggaaatcg
     6061 cggcgatgca tggagagatc atacccggcg tgccgccgct cacggccgga cgaacgaaag
     6121 cgaggcgcgc tccaagtctg cgcgcctcgc gcgagagccg gtcgtcccgt ccgatctact
     6181 tctgcccagg cgctctcacc tggacgctct ccgcgatcag aacgggacga ttctcgtagg
     6241 acgagaagaa gtcggcgtcg agcccgtagt cgctccagtc ggtgatgttg tgctcgcgcg
     6301 cgatgtcgtc cttcacgaac ggccggacct ttccgcgaac ctcgaccatc gagtccttgt
     6361 ccacgttcgc caggtccttc ttgctcagga tgagcagctt gtcgtccacg tcgaggacgc
     6421 ccttctcccg gatgtcgaac gcccgcttgc cgtgaacctc gttgacctcg cccgcgatgg
     6481 tgaccgtctg gttgtagaac tgctcggggt tgtcaacgat ggcctccacg gtggacatgg
     6541 cgccagccgt gccaacctgc ttctggctgt gaggagcctg ctgggtcggc tgggactgct
     6601 gggccggctg agactgctgg gctgcaaccg gcagcgccaa taccgccgcc agcgcgaccg
     6661 ctccaagtac cttcatcatg ttcctctcct tttctctctg attgcgtttc acgcctcaac
     6721 ggaccggttc acttcaatcg acgtaccagg ggctcaatgc cacggagaac aagggcatgg
     6781 actgatgaac cagagccaaa cacaatccga tgggcgaaag gacgaggttc gtgcacgagt
     6841 tgcaaccgcc gacaccggtc cccgctggtt cactgtttcg cggggaccgg tgggcgcggc
     6901 gttgctacct cttctgttcg agctggcgcc gcagctgctc gacgtggcgc tgaatcgcct
     6961 gcaggtcgtc gcgggaaatc gaaaccgcct cggacgggct gcccgaggtt ccgacctcac
     7021 gtccctcgag cagtgtctgc accgttcggg ctatgcggtc gagacgctct tcagcatcgg
     7081 ccctgccggc ggcccggcgg tcatcgctcc cggcgaggta cgcacgctgc gcctccccgc
     7141 tgtactgctg ccgcatgcca tccgcgccga cgaggaacac cgtgccggat gcctccgcct
     7201 cctctgatgc cagcacgtac tgctcggtcg cctcggcgat ctgcctcgcc tgcttctccg
     7261 gatacacgaa gaggtggccg gtctcctcgc ccggatagaa ccacgacttg atggccgggg
     7321 gctggtcacc gcgtgttccg acgaagctca ccttcacgcc gctggcgtcc tcggattcgg
     7381 gacggcggat gggcagcgtc accgtggtcg cgagtgccgg cccgccgtca ggcttgcgga
     7441 tctcgaccga ccgcgccggc tgcccggggt cgatcagctg aaagacgtac cggcctggcg
     7501 gcaacaccgc tcccggaacc tggaccgggc tgtcgaacgt gaagatggtg cggttctcga
     7561 actcctgagc ctgaacgacc ggcgccggtg caaaccacat cagccccgcg gccagcgccg
     7621 ccagcggcag ggcatgaatt gcgctccggc ttgctctctc tgcactactc gtcatcacac
     7681 ctccctgtgg tggattgcct tctctctctc ttctggatcg ttagcaaaac cctttccggc
     7741 aggaatctcg gggggaaggc agactgggca ggatgtgtgg ggcgctcgcg gggtgccgcg
     7801 cgcgatgcat gccgattccg ggctgattcg acctgatcgt cttacgcgcc gtgacgcctg
     7861 agcctctcga cgatctcctg gaactccctc tcctgtcgca gcggcgcgaa ggcgggctcg
     7921 gcgtcgatcg cggccccgtc gtcgaagccc ttcttgacgg cgctccgaag cgcgtcgatc
     7981 gctgcaccct tcttcccgtc gagagcgaga gcacgcgcac gttgatagtc aaccgccgca
     8041 tcggcgggcc ggatgcgcgc catcagcgaa aggcgttcgg cggcccgcgc cggccgcccg
     8101 gcctcgatgt cgcgcgcgtt ctcctcgttc atccagctcc acgacgatac caagacacgc
     8161 ctggccgcca tccgccctgc gtcacgtcgg ggccgctcgg cctgcgcccg catctcctcc
     8221 accgtccgca gaagctcctg catcgccagc gtccggtcct cgccctcgag cgcgtcgatc
     8281 acgacgcgcg caaagcgctc cttcgccgcc ttctcacgcg gcatcgagtc ggcttcctcc
     8341 ttcaccgcgg atcgcacctc gggcagcgcc cgcagctcgc gggccttgcg ctcgaacggc
     8401 gccacgtcgc gcaggccggc catcatcgcc gccagggcag agtagcggcg gtacgcttcg
     8461 accgcgcgtc ccgcttgctc ttcggcagcc gcctcgccgg acgctcgcgc gagaatccgg
     8521 tcgatcaggg tgtcgtccgc cccgcggctt ctcgtgcgga tcgcctggat ctccatccac
     8581 tcgatcgcgc ggacgcagaa ttccggcgag ggccaggaat
//
LOCUS       DAOPQX010000140         8593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9194_length_8593_cov_4.538299, whole genome shotgun sequence.
ACCESSION   DAOPQX010000140 DAOPQX010000000
VERSION     DAOPQX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9194_length_8593_cov_4.538299"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1101
                     /locus_tag="PKK95_08935"
     CDS             238..1101
                     /locus_tag="PKK95_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18379.1"
                     /translation="MPSPTAAVPRPRERLVIQPPGRVGSGLAGHLRSLPRYAELLYVL
                     TAHRVRVRYKQSALGLLWAVLQPLLLMLIFTMIYALIASRMSSGGLPYSLFVYAAILP
                     WTFFSTSVGNATGSLVGHSNLVTKVWFPRELLPISYVTAAVFDLAVASILLFGLMAWH
                     GVALAPLAWLALPIVALIALLALAIGLLAAAIQVRFRDIGLAIPLLLQVWMFLSPVIY
                     PFEQVPDRFKGLYSLNPMAGLIDGFRRVTVQGQAPDWALLSTSAVIVALLLPSSYMFF
                     KWVDATLADRM"
     gene            1104..2333
                     /locus_tag="PKK95_08940"
     CDS             1104..2333
                     /locus_tag="PKK95_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18380.1"
                     /translation="MADLVVSHLSKRYRIAGERRAPGGWLDRARAVLRGTAHDFWALH
                     DVSFEVARGETFGIIGRNGAGKSTLLKILSRVTAPTEGEVRILGRLAALIELGSGFHP
                     ELTGRENVFLNGAILGMRRREVAAKLDQIVEFAGVREFIDVPVKWYSSGMYVRLGFAI
                     AAHLEMDILLVDEVLAVGDAAFQARCFSRIAELKREGATIVIISHDLAAIERLCGRAL
                     LLDRGRVRRLGLARDVVAAYQRAVEGQPLEEAEAPPSRDVVIAAIEILDGGGTVTHRA
                     TTGEPVRIRIPYATAREVRAPRFELSFYSFDDGLLQSQCLGTAGGGMGDLAPGDGWAE
                     FEIGALGLQPGVYTLGATITERDAPRPCAWLYGRSTLYVEDGPGVAGRFFMPSAFTLR
                     APAARASDAGEAGSHAR"
     gene            2323..3258
                     /locus_tag="PKK95_08945"
     CDS             2323..3258
                     /locus_tag="PKK95_08945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC18381.1"
                     /translation="MRGEAESPVSVVIPCYNQARYLPEAIESALTQTAGRPEVIVVND
                     GSTEPVDLVVARYPGVHLIVQPNSGVAAARNTGLCMASRPLVIFLDADDRLTPEAVAT
                     GLAVLESRPEAAGAIGLCRVIDPEGRPRPFRQQPPIAGEPYAALLRSNFVWMPAEGIY
                     RREALLRIGGFDPSNPAAADYDLYLRLAREYALAVHRNVVAEYRVHDANMSANGLLML
                     RSTLCVLERQWPYARQRPEHRAAYAEGNRFWRDFYGDRVVEEIRASLKTAGRRRRAVR
                     WALALLRHHPRAVAVQLFRKLRNSVRRLVNPVSAG"
     gene            complement(3245..4543)
                     /locus_tag="PKK95_08950"
     CDS             complement(3245..4543)
                     /locus_tag="PKK95_08950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC18382.1"
                     /translation="MKGFPPAVTIVIPTRDRPVALARALHSLTRQDVPAADFEVVVVN
                     DGSGELPVAPADVERKLALRVVSNGGRGASAARNTGAAAARAPLLLFLDDDIEASPAL
                     VRAHLEAHRRGASLTVGYLPALPAAATGFFAATLRNWWETMFAEMRRPGHRFTFRDVL
                     TGNCAIDAALFRRAGAFDVSLACREDYELGARAIGAGAQLAFCEAAQGIHHEVTTLER
                     ALERKRAEGRADVAIIRKHPELLQALPLGATVGSHGPVPRGFRKLPFTRRRAASALAT
                     GLRRSLDLFEAARMRGRWHVRLYQLLELAYWQGIASEVGTLGALAALVHGTARADGRA
                     WLWLRVDLCKGLSPAERCVESVRPDAIAVFHRDETVGMISPWPGAEPLRGVHLRAALA
                     QTLAPPLLASLGRAGEIGVEQHGGRLVTSGPLPRGPFNPR"
     gene            complement(4540..5721)
                     /locus_tag="PKK95_08955"
     CDS             complement(4540..5721)
                     /locus_tag="PKK95_08955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC18383.1"
                     /translation="MATAVIDLELKHRSPGLRVPGGQPRALVLLRWAGRPVGQLTLPV
                     ENGAIEAERLERTLAQWAGPGFWTVWLRDGLGWDEAEPAPGPRAATVAVCTRERPEDL
                     ARCLNAVSALPQQPLDVVVVDNRPSTPRTRETVERFPGVRYVRQDEGGLNAARNRAVR
                     EARHDLVAFTDDDAIVDREWLAALVRNFDDPRVLCVTGLTMPVELETEAQELFEAYSP
                     FGRGFERRVFDAVRSSPLAVGRVGAGANMALRRSALDLVGEFDEALDGGTPTRSGGDH
                     EMFSRILAAGFRIVYDPAALSWHRHRRSMEELRETLRGYGTGVYAMWTRALVEGREYG
                     VFKQAAAWLRYGQVPELARALAGRPRRMPLSLLAAEWRGCLAGPFAYLASRRQAALRR
                     R"
     gene            5905..6054
                     /locus_tag="PKK95_08960"
     CDS             5905..6054
                     /locus_tag="PKK95_08960"
                     /inference="COORDINATES: protein motif:HMM:NF033488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HOC18384.1"
                     /translation="MLFTIAAILLVLWLLGFATGSVGSIIHIVLVIALVLFLVGLVSG
                     RRTVV"
     gene            complement(6174..7802)
                     /locus_tag="PKK95_08965"
     CDS             complement(6174..7802)
                     /locus_tag="PKK95_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18385.1"
                     /translation="MHRSMLITSLAFLLAGTPLLLGQAPTRPPRPASDLFRTSDGCQS
                     CHNQLTGPGGEDVSIGTEWRASIMANASRDPYWHGAVRREILDHPDAQAQIEHECSIC
                     HMPMTNYAARVAGGRGSVFAHLPIGSGQTPDNLLAADGVSCTVCHQVMPEKLGTRDSF
                     TGGYVVDAAVPYGQRAVYGPFDVKPGMAALMRSSAEFTPTRADHLNTSEMCATCHTLY
                     THALGKNGEVIGELPEQVPYLEWRHSAFAGQRTCQSCHMPTVKGPLSISSTLGEMRPS
                     LSRHEFRGANFFTPRLLNRYAAYLGVAALPQELDAASRRAADYLVTEAATLAVGPIEI
                     ADGRLKTEVVVENLGGHKLPTAYPSRRAWLHLTVRDEGGRMVFESGALETSGAIRGND
                     NDADPLRYEPHYREITAGGEVQIYEAIMADQAGAVTTGLLKGVRYLKDNRILPRGFDK
                     ATAVPDIAVRGPASADEDFQGGSDRVRLAVDLGGAQGPFTVEAELWYQPVGFRWARNL
                     ETYQAPEPQRFVRMYDGTAGASAVMLARVRVSGR"
     gene            complement(7817..>8593)
                     /locus_tag="PKK95_08970"
     CDS             complement(7817..>8593)
                     /locus_tag="PKK95_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOC18386.1"
                     /translation="AALTHVRGFCADEAWPRGELNLPRPIVTEKAFPEDEAVITTAIT
                     PRGGTSVANKLVYEKRIGPRSQVEIVLPYGFKDSGAGWVNGIGDVAFGFKRSLFHDHR
                     KGTIFSVTGEVVLPTGDEKDGFGKGYTVFEPFVTFGQILPGDGFVQFQGGIEVPSNSG
                     KAAKEAFWRTTLGKSISQNVFGRTWSPMVEIIGFRELEEGRSTHWDLVPQMQVTLSTR
                     QHIMANVGVRVPVNHREGRRTQLLFYLLWDWFDGPLFGGW"
BASE COUNT         1228 a         3010 c         3023 g         1332 t
ORIGIN      
        1 ggtcttgcgc cggtgcgact ccagccaggc acacgagagc ggcggcgacg ccaagcgccg
       61 tggccagtgg gcgaacgagt gtcgggttcc gcattcgttg atctccagtg acgacaaggg
      121 cggcagtcgc ggcgcgaggg ggccggaccg aaacttgtac tcgttttcgg cggggcgccg
      181 caaccaccgt ccgcgcaccg ctggcgccga agttgcacag cctagaggat cgccaagatg
      241 ccctcgccga ccgccgccgt gccgcggcct cgagaacggc tcgtgatcca gccgcccggc
      301 cgcgtcggct cgggtctggc cggccacctg cgctctctgc cccggtacgc ggaactgctc
      361 tacgtgctga cggcgcacag ggttcgggtc aggtacaagc agtcggcgct cggcctcctc
      421 tgggcggtcc tgcagccgct gctgctgatg ctcatcttca ccatgatcta cgcgctgatc
      481 gcgtcccgga tgtcgagcgg agggctgccg tactccctct tcgtctacgc ggccatcctt
      541 ccctggacct tcttctcgac ctcggtgggc aatgccaccg gcagcctggt cggccactcg
      601 aacctggtga cgaaggtctg gtttccgcgc gaattgctcc ccatctccta cgtcacggcg
      661 gctgtcttcg acctcgcggt cgcctcgatc cttctcttcg ggctgatggc gtggcacggc
      721 gtggcgctcg cgccactcgc gtggctggcg ttgccgattg tcgcgctcat cgcactgctg
      781 gcgctggcga tcggactgct cgcagccgcg atccaggtac gattccgcga catcggcctg
      841 gcgattcctc tcctgctcca ggtctggatg ttcctgagcc cggtcatcta ccccttcgag
      901 caggttccgg accggttcaa gggcctctac tcgctgaacc cgatggcggg gctgatcgac
      961 gggttccggc gagtcaccgt gcagggccag gcgcccgact gggccctgct ctcgacctcg
     1021 gcggtgatcg tcgccctgct gctgccctcg tcctacatgt tcttcaagtg ggtggacgcc
     1081 acgcttgccg accggatgtg acgatggccg acctcgtcgt ctcgcacctg tcgaagcggt
     1141 atcggatcgc cggcgagcgg cgcgccccgg gtggctggct cgaccgggcg cgcgccgtcc
     1201 tgcgagggac ggctcacgat ttctgggccc tgcacgacgt gtcgttcgag gtcgcgcgcg
     1261 gcgagacctt cggcatcatc ggccggaacg gcgccggcaa gagcacgctg ctcaagatcc
     1321 tgtcgcgcgt cacggcgccg accgagggcg aggtgcggat tctcggacgg ctggcggcgc
     1381 tgatcgagct cggatcgggc ttccaccccg agctgacggg gcgcgagaac gtcttcctga
     1441 acggcgcgat cctcggcatg cggcggcgcg aggtggccgc caagctcgac cagatcgtcg
     1501 agttcgcggg tgtccgcgag ttcatcgacg tgccggtcaa gtggtactcg tcggggatgt
     1561 acgtccgcct ggggttcgcg atcgcggctc acctcgaaat ggacatcctg ctcgtggacg
     1621 aggttctcgc ggtcggcgac gcggcgttcc aggcccggtg cttctcgcgc atcgccgagc
     1681 tgaagcggga gggagccacg atcgtgatca tctcgcacga cctggcggcg atcgagcggc
     1741 tgtgcggccg ggcactgctg ctcgatcggg gccgcgtgcg ccgcctcgga ctggcgcgag
     1801 acgtggtagc ggcctaccag cgtgcggtcg agggacagcc gctcgaggag gccgaggccc
     1861 cgccctcccg tgacgtcgtg atcgcggcca tcgagatcct ggacggagga ggcacggtca
     1921 cgcaccgcgc caccaccggc gagccggttc gcatccggat cccctacgcc accgcgcgcg
     1981 aggtgcgtgc gccgcgattc gagctgtcct tctattcctt cgacgacggg ctgctgcaga
     2041 gccagtgcct gggtaccgct ggaggaggga tgggcgacct cgcgccggga gacggctggg
     2101 ccgagttcga aatcggcgct ctcggcctcc agcccggcgt gtacacgctc ggcgcgacga
     2161 tcaccgagcg cgacgcgccg cgtccttgtg cctggctgta cgggcgctcg acgctctacg
     2221 tggaggacgg cccgggggtg gcgggcaggt tcttcatgcc ctcggccttc acgttgcgcg
     2281 cgcccgccgc tcgggcgagc gatgccggcg aagcgggatc ccatgcgcgg tgaggccgag
     2341 tcgcccgtct cggtcgtcat cccgtgctac aaccaggcgc ggtatctccc cgaggcgatc
     2401 gagagcgcgc taacccagac ggccggccgg cccgaggtca tcgtcgtgaa cgacggctcg
     2461 accgagccgg tcgacctcgt cgtggcccgg tacccgggcg tgcacctgat cgtgcagccg
     2521 aacagcggcg tggcggcggc ccgcaacacc gggctgtgca tggcctctcg gccgctcgtg
     2581 atcttcctgg acgccgacga ccggctcacg cccgaagcgg tcgccacggg cctggcggtg
     2641 ctcgaatcac gccccgaggc cgccggggcc atcgggctct gccgcgtgat cgacccagag
     2701 ggccggcccc gcccgtttcg ccaacagccg ccgatcgcgg gggagcccta cgcggcgctg
     2761 ctccgttcga acttcgtctg gatgcctgcc gaggggatct accggcgcga ggcgctcttg
     2821 cgaatcggtg ggttcgaccc gtcgaacccg gccgcagccg actacgatct gtatctgcgc
     2881 ctcgcccggg agtacgcgct ggccgtccac cggaacgtgg tggccgagta ccgcgtccac
     2941 gacgccaaca tgtcggcaaa cggcctgctc atgctgaggt ccacgctgtg cgtgctcgag
     3001 cgccagtggc cgtacgcccg gcagcggccc gagcaccggg cggcgtatgc cgagggcaac
     3061 cgcttctggc gcgatttcta cggcgaccgg gtcgtggagg agatccgcgc gtcgctgaag
     3121 acggcggggc ggcggcggcg ggccgtcagg tgggcgctcg cgctgctgcg gcatcacccg
     3181 cgagcggtcg ctgtccagct gttccggaag ctccggaaca gcgtccgccg cctggtgaac
     3241 cccgtcagcg cgggttgaag ggaccgcgcg gcaaagggcc cgaggtgacg agccggccac
     3301 cgtgctgctc gacgccaatc tccccggccc ggcccaggga cgcgagcaac ggcggtgcga
     3361 gcgtctgggc gagggccgcc cgcaggtgaa ctccgcgcaa cggctcggcg ccgggccacg
     3421 gcgaaatcat gccgaccgtc tcgtcgcgat gaaacacggc aatcgcgtcg gggcgcacgg
     3481 actccacgca ccgctcggca ggactcagcc ccttgcagag atccactcgc agccaaagcc
     3541 aggcgcggcc gtcggcccga gccgtcccgt gcacgagcgc ggcaagcgcg cccagggtgc
     3601 caacctcgga tgcgatccct tgccagtagg caagctcgag cagctggtag agccgcacgt
     3661 gccaccgccc ccgcatccgt gccgcctcga agagatcgag cgatcgtcga agccccgtcg
     3721 ccagggcgga ggcggcgcgc cgccgcgtaa agggcagttt ccgaaagccc cgaggcaccg
     3781 gtccgtgcga gccgacggtg gccccgagtg gaagcgcctg cagcagctcc ggatgcttcc
     3841 ggatgatggc cacgtcggcc cggccttcgg cccgcttccg ttcgagcgcc cgctcgagcg
     3901 tcgtcacctc gtggtggatc ccctgggccg cctcgcagaa cgccagttgg gcgcctgccc
     3961 cgattgcgcg cgcccccagc tcgtagtcct cacggcaggc gagcgagacg tcgaaagcgc
     4021 cggcgcggcg gaagagtgcg gcgtcgattg cgcagttgcc cgtcagcacg tcgcggaagg
     4081 tgaaacgatg gccgggccgc cgcatctccg cgaacatcgt ctcccaccag ttgcggaggg
     4141 ttgccgcgaa gaaaccggtc gccgccgccg ggagagcggg cagatagccc accgtcaggg
     4201 aagcgccgcg gcggtgcgct tccaggtgcg cgcgcacgag cgccggcgac gcttcgatgt
     4261 cgtcgtcgag aaacagcagc agcggcgcac gggccgcggc ggcccccgtg tttcgagcgg
     4321 cagacgcgcc tcgtccgccg ttcgaaacga cgcgcagggc gagcttcctc tcgacgtccg
     4381 ccggcgcgac cggcagttcg ccgctcccgt cgttcacgac cacgacctcg aaatcggcgg
     4441 ccggcacgtc ctgccgggtg agcgagtgaa gggcgcgagc gagcgccacg gggcgatcgc
     4501 gcgtggggat cacgatcgtg acagcgggag ggaaaccctt catcggcgtc gcagcgccgc
     4561 ctggcggcgt gacgcgaggt aggcgaatgg cccggccagg cagccgcgcc attcagcggc
     4621 gaggaggctg agcggcatcc gccgggggcg gcccgcgagc gcgcgagcca gctcggggac
     4681 ctggccgtag cgaagccacg ccgccgcctg tttgaaaacg ccgtactctc gcccttccac
     4741 gagcgccctg gtccacatcg cgtagacgcc cgtgccgtag ccgcgcagcg tctcgcgcag
     4801 ctcttccatg ctccgccggt ggcggtgcca gctgagtgcc gccgggtcgt agacgatccg
     4861 aaagcctgcg gcgagaatgc gcgagaacat ctcgtggtcg ccgcccgatc gcgtcggagt
     4921 gcccccgtcg agcgcctcgt cgaactcgcc gaccaggtcg agagccgagc gccgcagcgc
     4981 catgttagcg ccggcaccca cccgcccgac ggcgagcggg ctcgaccgta cggcgtcgaa
     5041 cacgcgccgt tcgaagcccc ggccgaatgg ggagtaggcc tcgaacagct cctgggcctc
     5101 ggtctccagc tcgaccggca tcgtgaggcc agtcacgcag aggacccgcg ggtcgtcgaa
     5161 attgcgcacc agcgccgcga gccactcgcg atcgacgatc gcgtcgtcgt cggtgaacgc
     5221 cacgagatcg tggcgcgcct cccggaccgc ccggttgcgg gccgcgttga ggccgccctc
     5281 gtcctggcga acgtagcgga caccggggaa gcgctcgacc gtttcccggg tccggggggt
     5341 tgacggacgg ttgtccacca cgacgacgtc gagaggttgc tggggaaggg cggacaccgc
     5401 gttcaggcag cgcgcgaggt cctcgggacg ttcgcgcgtg cacacggcca cggtcgccgc
     5461 gcggggccct ggcgcaggct ccgcctcgtc ccagcccagg ccgtcgcgca gccacaccgt
     5521 ccagaaaccc ggcccggccc attgggcgag tgtgcgctcg agccgttccg cctcgatcgc
     5581 cccgttctcg acgggcagcg tcaactggcc caccggccgc cccgcccatc gaagcagcac
     5641 cagggcgcgc ggctggccgc cgggcacccg aaggccggga gatcgatgtt tcagctccag
     5701 gtcgatgaca gccgtggcca tctgcgcaga attcgccacg ttcaatgcaa gagctctgcc
     5761 gccactgtgt gaggagtggc acaggcgtct cgcctgtgtg agcagtaccc cgcctgtgtg
     5821 aggagtggga cggccctccc ggccggcgtt caggccatcc ccggcggtac gtctcctgca
     5881 ggagaggcgg gaggaggagg tgacatgctg tttacgatcg ccgcgatcct gttagtgctg
     5941 tggcttctcg gtttcgcgac cggatcggtc gggagcatca tccacatcgt gctggtgatt
     6001 gcgctcgtgc tgttcctggt cgggttggtc agtggccgcc gcacggttgt ctgagcgagg
     6061 cggggtcgtc cgcagcgacg ttccgcgcgc gcgccccccg ctgcggaccg gggtaaggtc
     6121 cctggccctc gccctcccga gtgggaagcg aggttccagg gaccggtcga gccttacctg
     6181 cccgatactc tcaccctcgc cagcatgacg gccgacgcgc cggccgtccc gtcgtacatc
     6241 cgcacgaagc gttgcggctc cggtgcctgg taggtttcga ggttccgcgc ccagcggaag
     6301 cccaccggct ggtaccacag ttcggcctcg acggtgaaag gtccctgcgc tccgcccagg
     6361 tccacggcca gccgcacccg gtcggatccg ccctggaagt cctcgtctgc tgatgccggc
     6421 ccacgtacgg cgatgtccgg gaccgcggtc gccttgtcga agccgcgggg gaggatccgg
     6481 ttgtccttca ggtaccgcac ccccttcagc agcccggtcg tgacggcacc ggcttggtcg
     6541 gccatgatcg cctcgtagat ctgcacctcg ccgccggcgg tgatctcacg gtagtggggc
     6601 tcgtagcgca agggatcggc gtcgttgtcg ttgccgcgga tggcgccgct cgtctcgagc
     6661 gcacccgact cgaacaccat gcggccaccc tcgtcgcgga ccgtgaggtg cagccacgcc
     6721 cgtcgcgagg gatacgcggt cggcagcttg tgcccgccga ggttctcgac gaccacctcg
     6781 gtcttcagcc ggccgtcggc gatttcgatc gggccgacgg caagagtggc ggcctcggtc
     6841 accaggtagt cggccgctcg acggctggcc gcgtcgagtt cctggggcag ggctgcgacg
     6901 cccaggtacg ccgcgtagcg gttcaggaga cggggcgtga agaagttggc accgcggaac
     6961 tcgtgccgcg agaggctggg gcgcatctcg cccagggtgg aactgatgga caacggcccc
     7021 ttgacggtgg gcatgtggca cgactggcag gtgcgctgcc cggcgaacgc gctgtgccgc
     7081 cattcgaggt acgggacctg ctcgggaagc tcgccaatca cctcgccgtt cttgcccagc
     7141 gcgtgcgtgt acagcgtgtg gcacgtggcg cacatctccg acgtgttcag gtgatcggca
     7201 cgggtgggcg tgaactccgc tgaggaacgc atcagcgccg ccatgcccgg cttgacgtcg
     7261 aagggtccgt agacggcccg ctgcccgtac ggcacggcgg catcgacgac gtagccgccc
     7321 gtgaagctgt cgcgggtccc cagcttctcc ggcatcacct ggtggcagac ggtgcacgag
     7381 acgccgtcgg ccgcgagcag gttgtcgggc gtctgcccgc tcccgatcgg caggtgcgcg
     7441 aacaccgacc cccggccgcc cgcgacgcgg gcggcgtagt tggtcatcgg catgtggcag
     7501 atcgaacact cgtgttcgat ctgcgcctgg gcgtctggat ggtcgaggat ctcccgccgc
     7561 acggcgccgt gccagtaggg atcgcgcgac gcgttcgcca tgatcgacgc ccgccactcc
     7621 gtgccgatgg agacgtcctc gccgcctggt cccgtcagct ggttgtggca cgactggcag
     7681 ccgtcggagg tgcggaacag gtcggatgcc ggacggggag ggcgggtggg ggcctgcccc
     7741 aagaggagcg gcgtgccggc caggaggaag gcgaggctgg tgatgagcat cgagcggtgc
     7801 ataggtctcc tccctcctac cacccaccga acaaggggcc gtcgaaccag tcccagagca
     7861 ggtagaagag cagctgcgtg cggcggccct cgcggtggtt gaccggcacg cgcacaccga
     7921 cgttggccat gatgtgctga cgcgtgctga gcgtgacctg catctgcggc acgaggtccc
     7981 agtgcgtgga ccggccctcc tccagctcgc ggaacccgat gatctcgacc atcggcgacc
     8041 aggtccggcc gaacacgttc tggctgatgc tcttgcccag cgtggtgcgc cagaaggctt
     8101 ccttcgccgc cttccccgag ttgctcggga cctcgatgcc gccctggaac tgcacgaacc
     8161 cgtcgcccgg gaggatctgc ccgaaggtga cgaagggctc gaagacggtg tagcccttgc
     8221 caaagccgtc cttctcgtcg cccgtcggaa gcacgacctc cccggtgacg ctgaagatcg
     8281 tgcccttgcg gtggtcgtgg aagagcgagc gcttgaagcc gaacgccacg tcgccgatgc
     8341 cgttcaccca gcccgccccc gagtccttga agccgtacgg caggacgatc tcgacctggc
     8401 tgcgcgggcc gatgcgcttc tcgtagacca gcttgttggc gaccgaggtc ccgcccctcg
     8461 gagtgatggc cgtggtgatc accgcctcgt cctcggggaa cgccttctcg gtgacgatcg
     8521 ggcgcgggag gttgagctcg ccccgaggcc acgcttcgtc ggcacagaat ccgcgcacgt
     8581 gcgtcaacgc cgc
//
LOCUS       DAOPQX010000141         8579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9210_length_8579_cov_4.488972, whole genome shotgun sequence.
ACCESSION   DAOPQX010000141 DAOPQX010000000
VERSION     DAOPQX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9210_length_8579_cov_4.488972"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /gene="priA"
                     /locus_tag="PKK95_08975"
     CDS             <1..1907
                     /gene="priA"
                     /locus_tag="PKK95_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOC18387.1"
                     /translation="ATVAGLSALEAIGAGGEEAIALGPRQLGALRALAAAPDGLAVPA
                     LREHGAGADVVQRLAARGLVSIREERLDRDPFAASAWTHGPNAGTPRGSVALTGEQSD
                     ALARLGSLARQRAFAVALLHGVTGSGKTEIYLRLASEVVAAGRSALVLVPEIGLTPAL
                     AGVFAAAFGGRVAIQHSGLSDGERHDQWHRIQRGEVDVVVGTRSAVFAPLPSPGLIVV
                     DEEHDASYKQEEAPRYHGRDVAVMRGKREGALVVLGSATPSLESYHNARAGRYELITL
                     ERRVLDRPLAAVSLVNMREEYALRGPEAILSQRLVEAIGDRLQRREQVLVLLNRRGYA
                     TAILCRQCAGTIDCPNCSVSLTIHVGARGARRGRCHYCNYSAAIPAACPSCAAPYLEQ
                     AGFGTERVEREVLAAFPSSRVARFDRDTIRRRGAAVAMLERFGAGEIDVLVGTQMIAK
                     GHDFPRVTLVGVVSADVGLGLADFRAPERTFQLLTQVVGRAGRGEVGGEAIIQTLHPE
                     HYGIRHACRQDYPAFFEEELAFRRAMRYPPFVALTNGIVRGPSLAAAMHDAGRLVRGL
                     REQPGGSSFQVLGPAPAPFVKLRGEHRAQFFIKGANRRAMREAVRAVVEAHPDLRRRL
                     SIDVDPATVL"
     gene            complement(1989..2600)
                     /locus_tag="PKK95_08980"
     CDS             complement(1989..2600)
                     /locus_tag="PKK95_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18388.1"
                     /translation="MKAWTGALIAVLTIGLMVFGLGMRSPSSGVAGQAESLARMPQAN
                     GLAQPGAVAAVAATGLQAAEATQAPMLVNCGPGQQALVRQVTLANGQPASQVDCVGVA
                     GSAAAYAAAQPAYAAPQVRMAADDDIVTAPRVVRTAETYRASAPTRQVEKPRRSWKKT
                     ALIIGGSTAAGAGVGGIVAGKKGALIGAAIGGGASTIYEAVKR"
     gene            complement(2787..5045)
                     /locus_tag="PKK95_08985"
     CDS             complement(2787..5045)
                     /locus_tag="PKK95_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC18389.1"
                     /translation="MIGMRRVRIAILLLSCGVGAAAATGAAGQERAGGKKVWFTYDQV
                     FSVPARPAEGAKPASSVLAPLPEIMGWADADHFLEAREDPSDRQRKVYAVGAEDSSAR
                     LYRDYSEVQKALPAGFEAQAAAATMPDLSAFVFLQGGDLYHYDAAAKRFRRLTATLAD
                     ERNPRFSPDGRWVAYTRTNDLFAYDLVNAVEHQYTNDGSDTVYNGWASWVYMEEILGR
                     ATNSAAFWWSPDSTRLAFMRFDDSPVPVFPIYRADGQHGELERQRYPKAGDPNPWVRM
                     GVVPVADGGVVWMDFEEKADHYIAWPFWTKDGKTLTVQWMNRGQDVIRFYNCDPATGK
                     KVQIFEEKQPAWVEFFEDLYHFQDGSGFLLRSTVDGWDHLYYHRTDGTLRQRLTSGEW
                     RVQGIAAVDEAGGWVYFVARYGKPWDTHLMRVRLDGSGLQQLTKEPGAHSVRVAPGGK
                     YFVDTFSSISTAPAMALYRGDGTLVRKLGDAWTPEAARYAWGKAELFTISSEDGAFQL
                     PAYWVLPPDFDPRKQYPVVFSIYGGPDAGTVRNAWLGLRSHYWAQRGVITIGVDHRGG
                     GAFGKKGVALMHRNLGKWEMIDLVTAAKWLRAKPFVAKDRIGIEGGSYGGYTTMMALF
                     HGAGHFNFGQAGSSVSDWRLYDTVYTERYMDTPEENPEGYRTGAVLTYGDRYKGGLLI
                     THGTIDDNVHLQNSMQVVDYLTTANKPFELMLYPDSRHGIQAAQRPHYSRVAHDYWVK
                     TLLGGRLPAGPR"
     gene            complement(5067..5717)
                     /locus_tag="PKK95_08990"
     CDS             complement(5067..5717)
                     /locus_tag="PKK95_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOC18390.1"
                     /translation="MGAVSSCSLTPAACRRAVRHLGGADPVMRVIIERHGPCRLGVAG
                     KGPVLQALTRALVSQQLSVKAADTIYTRFLGLFPGDGFPQPAAILAVPAEQLREVGLS
                     RPKACYLRDLCGRVLSGELPLDRLDAMSDEEVLATLTAVKGIGRWTAEMILIFQLRRP
                     DILPVDDVGILRGIQRAYGLRTRPSPARVLRLGEKWRPYRSVASWYIWQSLDSTPG"
     gene            5893..8025
                     /locus_tag="PKK95_08995"
     CDS             5893..8025
                     /locus_tag="PKK95_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC18391.1"
                     /translation="MSDHRIREHPILPVPEARAVPFSWQGQPFEAREGETIASALFAN
                     GVRTFGHHHKDGAPQGIFCANGQCAQCTVIADALPVKACMVPVRAGMAVEPLDGLPVV
                     PDVEGHPRTHPIETVEVECLVVGGGPAGLTAAIELGKAGVRTLIVDDKHRLGGKLVLQ
                     THKFFGSIEACHAGTRGIDIATRLEKEVRGFEHVRIWLNSTVLAVFSDRRVGILREGQ
                     YFVVRPDVLLVAAGAREKSLVFPGNTLPGVYGAGAFQTLVNRDLVRPTGRLFVIGGGN
                     VGLIAAYHALQAGIAVVGLCEALPECGGYKVHRDKLARMGVPIYTSHTIVRAEGDREV
                     SGAVIAQVDRNWKVVPGTEKRFECDTILVAVGLDPVNEFLEKAREFGLPAVAAGDSEE
                     IAEASAAMFTGRIRGLEVARRLGRDVGEVPPEWHRTAEVLKSRPGMTITEYLPEKREG
                     VFPIFHCSQEIPCNPCTSICPNHSIVIAGDAVMGLPEFVGDACDGCEKCVAICPGLAV
                     TLVDYRKSADRPTVVIPYEFSSRSIKKGDVVTVLDSEGAVLGNLEVTRVRAPRDADRA
                     LLVRVAAPADIAPRIAGIRVQEPWEPARPETASRPIGDDEIVCRCERVTARQLRELIR
                     SGIRDMNHLKASTRCGMGACGGKTCPNLIKRLFREEGVPLEEVADLTRRPLFMEVPLG
                     AFAGVVEGEAPIKDVPRRHATDAHEGGM"
     gene            8025..>8579
                     /locus_tag="PKK95_09000"
     CDS             8025..>8579
                     /locus_tag="PKK95_09000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC18392.1"
                     /translation="MGAQVYDVIVVGAGSVGLPTAVSLAEAGLTTLVVDQFAGPGQGS
                     NKAAIGGIRATHSAPGKIHLCLESLRLFSNWQALHGDDIEWFQGGYVFVAYREQEERA
                     LKDLLQVQQRHGLNIRWLDRESLVELVPAIAREGLRGGTYSPEDGSASPLMSSAAFYR
                     RAVELGVQFRKAKMLYMQEQTLRKV"
BASE COUNT         1222 a         3067 c         3002 g         1288 t
ORIGIN      
        1 ccgcaaccgt cgccgggttg tcagccctcg aggcgatcgg cgccggcggg gaagaggcga
       61 tcgccctcgg gccacgccag ctgggcgcgc ttcgcgccct ggccgccgcg ccggacgggc
      121 tggcggtgcc ggcgctgcgt gagcacggcg cgggcgccga cgtggtgcag cggctggcgg
      181 cgcgcgggct cgtctccatc cgagaagagc ggctcgaccg cgacccgttc gccgcgtcgg
      241 cgtggacgca cggccccaat gcagggacgc cgcgggggag cgtggccctc accggcgagc
      301 agtctgacgc gctcgcgcgg ctcggctcgc tcgcgcggca gcgcgccttc gcggtagcgc
      361 tgctgcacgg cgtgacgggc agcggcaaga cggagatcta cctgaggctg gcctcggaag
      421 tggtggccgc cgggcgctcg gcgctggtac tggtgccgga aattggcctc accccggcgc
      481 tggccggcgt gttcgccgcg gcgttcggcg ggcgggtggc gatccagcac agcgggctgt
      541 cggacggcga gcgtcacgat cagtggcacc ggatccagag gggcgaggtg gacgtggtgg
      601 tgggcacccg gtcggcggtc ttcgcgccgc tgccgtcgcc cggcctgatc gtggtggacg
      661 aggagcacga cgcctcctac aagcaggagg aagcgccgcg gtaccacggc cgcgacgtcg
      721 ccgtgatgcg cggcaagcgc gagggggcgc tcgtggtgct cggctcggcg acgccgtcgc
      781 tcgagagcta ccacaacgcc cgcgccgggc ggtacgagct gatcacgctc gagcgccgcg
      841 tgctcgaccg gccgctcgcg gcggtgtcgc tggtgaacat gcgcgaggag tacgcgctgc
      901 gcggccccga ggcgatcctg agccaacggc tcgtcgaggc catcggcgac cggctgcagc
      961 gccgcgagca ggtgctcgtg ctgctgaacc gccgcgggta cgccaccgcc atcctctgcc
     1021 gccagtgcgc cggcaccatc gactgcccga actgcagcgt ctcgctgacg atccacgtcg
     1081 gtgcacgcgg cgcccggcgc ggccgctgcc actactgcaa ctactcggcg gcgatcccgg
     1141 ctgcctgccc gtcgtgcgcc gccccctacc tcgagcaggc cgggttcggc accgagcggg
     1201 tggagcgcga ggtgctggcc gccttcccgt cctcacgcgt ggcacgcttc gatcgcgaca
     1261 cgatccggag gcgcggcgcc gccgtcgcga tgctcgaacg cttcggcgcg ggcgagatcg
     1321 acgtgctcgt gggcacgcag atgatcgcca agggacacga cttcccgcgc gtgacgctgg
     1381 tgggcgtggt gtcggccgac gtgggcctcg gcctcgccga cttccgggcg cccgagcgga
     1441 ccttccagct gctgacgcag gtggtgggac gggccggccg gggcgaggtc ggcggcgagg
     1501 cgatcatcca gacgctccac ccggagcact acggcatccg gcacgcctgc cggcaggact
     1561 acccggcgtt cttcgaggag gagctggcct ttcggcgggc gatgcgctac ccgccgttcg
     1621 tcgccctcac caacggcatc gtgcgcggcc ccagccttgc ggcagcgatg cacgacgcgg
     1681 gcaggctggt gcgaggcctg cgcgagcagc ccggcggctc gagcttccag gtgctcggcc
     1741 cggcgccggc gcccttcgtg aaactgcgcg gcgagcaccg ggcgcagttc ttcatcaagg
     1801 gcgccaaccg gcgtgcgatg cgcgaggcgg tccgcgccgt cgtcgaagcc caccccgacc
     1861 tccgccgccg gctgtcgatc gatgtcgatc cggccacggt gctctgaccg ccccctagct
     1921 cctagcccct agcccctagc ccctagcccc tagctcctag cccctagccc ctagccccta
     1981 gcccctagct aacgcttcac cgcctcgtag atggtgctgg cgccgccgcc gatggccgcc
     2041 ccgatgagcg cgcccttctt gccggcgacg attcccccga cgccggcgcc cgcggcggtc
     2101 gatccaccga tgatgagcgc cgtcttcttc cagctgcgcc gtggcttctc cacctgccgg
     2161 gtgggcgcgg acgcccggta ggtctcggca gtcctcacga cgcggggcgc ggtcacgatg
     2221 tcatcgtcgg cggccatccg cacctgcgga gcggcgtagg caggctgggc ggccgcgtac
     2281 gccgccgcgc ttcccgccac gccgacgcag tcgacctgcg acgccggctg cccgttggca
     2341 agcgtcacct gtcgcacgag cgcctgctgg ccggggccgc agttgacgag catcggtgcc
     2401 tgcgttgcct cggcagcctg caggccggtc gcggcgacgg ccgccaccgc tccaggctgc
     2461 gccagcccgt tcgcctgcgg catgcgcgcc aggctctcgg cctgccccgc cacgccgctc
     2521 gacggggaac gcatcccgag gccgaacacc atcaggccga tcgtcagaac cgcaatcagg
     2581 gctccggtcc acgccttcat gacatccccc cttggtctgg cgccgccgcc ccgatggccg
     2641 cgacgcgtta ccccccaggg aacaaggacg atgccagcgg caaatcaccc tgattcgagc
     2701 gattttcctc cagacttcgc ccgccggcgc cccgcggcac cgacaggccg tcacctgcag
     2761 gggtcagccg tccccgctcg ctgtccctac ctcggccccg cgggcaacct tcccccgagc
     2821 agcgtcttca cccagtaatc gtgcgcgacg cgcgagtagt gcgggcgttg cgcggcctgg
     2881 atgccgtgcc ggctgtcggg gtagagcatc agctcgaacg gtttgttcgc agtggtcagg
     2941 tagtcgacca cctgcatcga gttctgcagg tgcacgttgt cgtcgatcgt gccgtgcgtg
     3001 atcagcaggc cgcccttgta gcgatcgccg taggtcagga cggcgcccgt ccgatagccc
     3061 tcggggttct cctcgggcgt gtccatgtag cgctcggtgt agaccgtgtc gtagagccgc
     3121 cagtcgctga ccgacgaacc cgcctggccg aagttgaagt gaccggcgcc gtggaacagc
     3181 gccatcatcg tcgtgtaccc gccgtagctc ccgccctcga tcccgatccg gtcctttgcg
     3241 acgaacggct tcgcgcgcag ccacttcgcc gccgtgacca ggtcgatcat ctcccacttc
     3301 cccaggttcc ggtgcatcag ggcgacgccc ttcttgccga aggcgccgcc gccgcggtga
     3361 tcgacgccga tcgtgatgac gcctcgctgc gcccagtagt ggctgcgcag cccgagccag
     3421 gcgttccgga cggtgccggc gtcggggccg ccgtagattg aaaagaccac ggggtactgc
     3481 ttcctcggat cgaagtcggg cggcagcacc cagtaggcgg gcagctggaa cgccccgtcc
     3541 tcggacgaaa tcgtgaacag ctcggccttc ccccaggcgt aacgcgccgc ctcgggggtc
     3601 caggcgtcgc cgagcttgcg caccagcgtc ccgtcgccgc gatagagggc catcgccggg
     3661 gcggtcgaga tcgagctgaa cgtgtcgacg aagtacttgc cgcctggcgc gacgcgcacc
     3721 gagtgcgcgc caggctcctt ggtgagctgc tgaagcccgc tgccgtcgag ccggactcgc
     3781 atgaggtgcg tgtcccacgg cttgccgtac cgtgcgacga agtacaccca gccacccgcc
     3841 tcgtccaccg cggcgatgcc ctggacgcgc cactcgccgc tggtgagccg ctgcctgagg
     3901 gtgccgtcgg tccggtggta gtaaaggtgg tcccacccgt ccaccgtgct ccgcagcagg
     3961 aagcccgagc cgtcctggaa gtggtacaag tcctcgaaga actccaccca cgccggctgc
     4021 ttctcctcga agatctgcac cttcttcccg gtcgccgggt cgcagttgta gaacctgatc
     4081 acgtcctggc cgcggttcat ccactgcacc gtcagggtct tgccgtcctt cgtccagaac
     4141 ggccacgcga tgtagtggtc ggccttctcc tcgaagtcca tccagacgac gccgccgtcg
     4201 gctaccggga cgacgcccat gcgcacccag gggttcggat cgccggcctt ggggtagcgc
     4261 tgccgctcga gctcgccgtg ctggccgtcg gcgcggtaga tcgggaacac gggcaccggc
     4321 gagtcgtcga agcgcatgaa cgcgagcctc gtgctgtcgg gcgaccacca gaatgccgcg
     4381 gaattggtgg cgcggccgag gatctcctcc atgtagaccc acgacgccca cccgttgtag
     4441 acggtgtcgc tgccgtcgtt cgtgtactgg tgctcgaccg cgttcaccag gtcgtacgcg
     4501 aagaggtcgt tggttcgcgt gtaggcgacc catctcccgt cgggcgagaa gcgggggttc
     4561 ctctcgtcgg ccagcgtcgc ggtcagccgg cggaagcgct tcgccgccgc gtcgtagtga
     4621 tagaggtcgc cgccctgcag gaacacgaag gccgacaggt cgggcatcgt cgccgcggcc
     4681 gcctgcgctt cgaaccccgc aggcagggcc ttctgaacct cgctgtagtc gcgatacagc
     4741 cgggctgagc tgtcctcggc tccaacggcg tagaccttgc gctgcctgtc ggacgggtcc
     4801 tctcgcgcct cgaggaagtg gtcggcgtcg gcccagccca tgatctccgg cagcggggcg
     4861 agcaccgacg aggccggctt cgcgccttcg gcgggccgcg ccggaacgct gaacacctgg
     4921 tcgtaggtga accagacctt cttcccgccg gcgcgctcct ggccggccgc accggttgcc
     4981 gccgccgcgc cgacaccgca agaaagtagc aggatggcaa tccgcacgcg tcgcattccg
     5041 atcatcgaca cctccgaaat caccctctat ccaggggtgc tgtccaggct ctgccagatg
     5101 taccagctcg ccaccgacct gtaaggacgc cacttctccc cgaggcgcag cacgcgcgcg
     5161 ggcgagggac gcgtgcgcag gccgtaggcc ctctggatgc cgcggagaat ccccacgtcg
     5221 tccaccggca ggatgtcggg ccgccggagc tggaagatga ggatcatctc ggccgtccag
     5281 cggccgatcc ccttcaccgc ggtgagcgtg gccagcacct cttcgtcgga catcgcgtcg
     5341 aggcggtcga gcgggagctc gccgctcagc acgcggccgc agagatcgcg caggtagcag
     5401 gccttcggcc ggctgaggcc gacctccctc agctgttcgg ccggaacggc gaggatggcc
     5461 gccggctggg ggaagccgtc gccggggaac aaccccagga agcgcgtgta gatcgtgtcc
     5521 gcggccttga cggagagttg ttgcgagacc agcgccctgg tcagcgcctg cagaaccggc
     5581 cctttgccgg cgacaccaag gcggcacgga ccgtgccgct cgatgatgac gcgcatcacg
     5641 ggatcggcgc cgcccaggtg acgaacggcg cgccggcagg cagcgggcgt cagcgagcag
     5701 gaggataccg cacccatccg gctattctgc ctcatctcga cgcctgactt cgtcgacttc
     5761 gcgactcgcg acctcgtgac ttcgcgactt cgtggagtga tttcctgtga tatcctgccc
     5821 tcgtcggtgt tcccccccgc cgccggtctt cagcaggcac cagccctcac atcgttctgc
     5881 gggagtatcc ccgtgtccga ccaccgcatc cgtgagcatc cgatccttcc tgtgcccgag
     5941 gcgcgcgccg tgccgttcag ctggcagggg cagccgttcg aggcgcgcga gggtgaaacg
     6001 atcgcctccg cgctcttcgc gaacggcgtg cgcaccttcg gccaccacca caaggacggc
     6061 gccccgcagg gcatcttctg cgccaacggc cagtgcgcgc agtgcacggt gatcgccgac
     6121 gccttgccgg tgaaggcgtg catggtgcct gtccgcgccg gcatggcggt cgagccgctc
     6181 gacggcctcc ccgtcgtgcc cgacgtcgag gggcatccgc gcacgcaccc gatcgagacg
     6241 gtggaggtcg agtgcctcgt cgtcggcggc gggccggcgg gcctcacggc ggccatcgag
     6301 ctgggcaagg cgggggtccg cacgctcatc gtggacgaca agcaccggct cggcggcaag
     6361 ctcgtcctcc agacccacaa gttcttcggg tcgatcgaag cgtgccacgc gggcacgcgc
     6421 ggcatcgaca tcgccacgcg gctcgagaag gaggtgcgcg gcttcgagca cgtccgcatc
     6481 tggctgaact cgacggtgct ggcggtcttc tccgatcggc gcgtcggcat cctccgagaa
     6541 ggccagtact tcgtcgtccg gcccgacgtc ctgttggtgg cggccggcgc ccgcgagaag
     6601 agcctcgtct tccccggcaa cacgctgccc ggcgtctacg gggcgggtgc gttccagacg
     6661 ctcgtgaacc gcgacctcgt caggccgacc gggcggctgt tcgtcatcgg cggcggaaac
     6721 gtggggctga ttgccgcgta ccacgcgctg caggccggca tcgcggtcgt cgggttgtgc
     6781 gaggcgctgc ccgagtgcgg cggctacaag gttcaccggg acaagctcgc gcggatgggc
     6841 gtccccatct acacctcgca caccatcgtc cgcgccgagg gcgaccgcga ggtgagcggc
     6901 gcggtcatcg cgcaggtcga ccggaactgg aaggtcgtcc cgggcaccga gaagcgcttc
     6961 gagtgcgaca cgatcctcgt cgcggtcggc ctcgacccgg tgaacgagtt cctcgagaag
     7021 gcgcgggagt tcgggctgcc cgcggtggcg gccggcgact cggaggagat tgccgaggcg
     7081 tcggcggcga tgttcaccgg gcgcatccgc gggctcgagg tggcgcgcag gctgggccgc
     7141 gacgtgggcg aggtgccgcc ggagtggcac cgcacggccg aggtgctcaa gtcgcgcccc
     7201 gggatgacga tcaccgagta cctgccggaa aagcgggagg gggtcttccc catcttccac
     7261 tgctcccagg agatcccctg caacccctgc acctcgatct gccccaacca cagcatcgtg
     7321 atcgcgggcg acgcggtgat gggactgccc gagttcgtcg gcgacgcctg cgacgggtgc
     7381 gagaagtgcg tcgcgatctg cccgggtctt gcggtgacgc tggtggacta ccggaagagc
     7441 gccgaccggc ccaccgtcgt catcccctac gagttctcgt cccgctcgat caagaagggg
     7501 gacgtggtca cggtactcga cagcgagggg gccgtcctcg gaaacctgga ggtgacgcgg
     7561 gtgcgcgcgc cgcgcgacgc cgaccgcgcg ttgctggtca gggtggcggc ccctgccgac
     7621 attgcgccgc gcatcgcggg catccgcgtg caggagccct gggagccggc ccggcccgaa
     7681 acggccagcc gcccgatcgg cgacgacgag atcgtgtgcc ggtgcgagcg ggtgaccgcg
     7741 cggcagctgc gcgaactgat ccgctcgggc atccgggaca tgaaccacct gaaggcgagc
     7801 acccgctgcg ggatgggagc ctgcggcggc aagacctgcc ccaacctgat caagcgcctg
     7861 ttccgcgagg aaggcgtgcc cctggaggaa gtggccgacc tgacgagacg gccgctgttc
     7921 atggaggtgc cgctcggcgc cttcgcggga gtggtcgagg gggaggcgcc gatcaaggac
     7981 gtgccgcggc gacacgcgac cgacgcgcac gaggggggca tgtaatgggc gcgcaggtct
     8041 acgacgtgat cgtggtcggc gcaggcagcg tcggccttcc caccgccgtg tcgctggccg
     8101 aggcgggcct cacgacgctc gtggtggatc agttcgcggg gccgggccag gggagcaaca
     8161 aggcggccat cggcggcatc cgcgccaccc attccgcgcc gggcaagatt cacctgtgcc
     8221 tcgaatcgct gcgcctcttc tcgaactggc aggccctgca cggggacgac atcgagtggt
     8281 tccagggcgg ctacgtcttc gtcgcgtatc gcgagcagga agaaagggcc ctcaaggacc
     8341 tcctgcaggt ccagcagcgg cacggcctca acatccggtg gctcgaccgc gagtcgctcg
     8401 tcgagctggt gccggccatc gcgcgcgagg ggctgcgagg cggcacctac tcacccgagg
     8461 acggctcggc ctctccgctg atgtcgtcgg ccgccttcta ccgccgcgcg gtcgagctgg
     8521 gcgtccagtt ccgcaaggcc aagatgctct acatgcagga acagacactt cgcaaggtc
//
LOCUS       DAOPQX010000142         8569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9224_length_8569_cov_4.844022, whole genome shotgun sequence.
ACCESSION   DAOPQX010000142 DAOPQX010000000
VERSION     DAOPQX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9224_length_8569_cov_4.844022"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2310
                     /locus_tag="PKK95_09005"
     CDS             <1..2310
                     /locus_tag="PKK95_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOC18393.1"
                     /translation="TEAEARKIAAESKPVVCMTMSMHATEIGGAQMSPELVYDLVTRQ
                     DAETRRILDNVVFLLVPSFNPDGQIMVTDYYNKYLGTEYEAGSLPWLYHKYVGHDNNR
                     DAMTMNMKESQYVGRLLFTEWKPQAYFDFHHMGSYGARMYFPPYAEPIRPLADPLVWT
                     ESAWYGGQMANKASEAGLTGVLNNGQYSGWGHFGFHWITPFHNIAGMLSEAASARLAT
                     PLYIDPSQLTGRSRNQQDPREPQMNFPDPWPGGWWRLRDIVDRQKVGAWALLDCMARN
                     AETIVWDTYVKAMRQTERGAKGKPAAYAISADQHDPLTLDKLVNALLAQGIEVLRSAK
                     GFAAPGGVVYPAGSFYVTLAQPKMGLVRYLLGETHYPENEWTRQQDGTPIRPYDMGQD
                     VLAEFMGIRVDPLDEAVEADFTRVAAPILRAGTVEKGPAGYVIDGRLNDAFRAVNLLF
                     DRDVTVRRADAAGDAVRAGDFLLPASAPAAVLAEVAKTTGVDLRALKAEPKAAHELKR
                     LRIGMYQRYRGGNMDEGWTRWIFEQFGFQYKSVLDGEIKKGNLRASYDVFVFADDSTS
                     AITGEPAPSRPGGFSRGARFEGVPDTTPPEYKSGIGADGVAAIKEFVRAGGTLVTLGG
                     ATDFAISKLGLPVRNVVEGLSTKEFFCPGSTLRVTIDNAHPLAYGMPAEGLVLFHYSP
                     AFDVVATDFNHRNDVVVRYAERDVERSGWLNGEEHIAGKTAMASIGYGEGRVVLIGFR
                     TQNRAQTLGTYKFLFNALVTPPPAARNQP"
     gene            2415..3230
                     /locus_tag="PKK95_09010"
     CDS             2415..3230
                     /locus_tag="PKK95_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC18394.1"
                     /translation="MATGIERKTCQAPDGVTIGYSVVGKGEPALLFVHGAMADRSFYD
                     SQLQALSDRWRVIAVDLAGHGESGVNRTTWGIREFGADVKAVADAEGLKRLVVFGNSL
                     GGPVAVEAALLLPGRAVGVVGIDTFQDLGQAASLEYRRAADEYYHKRAESFRADYPVA
                     LREMVRMLFHPDADPDLVAEVERRMLRTPAPVAAATLDSLPGYDQEAAVRKLNVPLRS
                     ITGDLFPTDLQKARAVTPDFDVIVMKHIGHYPMLERPGEFNRHVAEMVARLAR"
     gene            complement(3437..3598)
                     /locus_tag="PKK95_09015"
     CDS             complement(3437..3598)
                     /locus_tag="PKK95_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18395.1"
                     /translation="MLLVRAAILVLFAGFAYLVTRSFSPFAGVLTVAAAGGLLIAFLM
                     IFLRRGGRS"
     gene            complement(3614..4633)
                     /locus_tag="PKK95_09020"
     CDS             complement(3614..4633)
                     /locus_tag="PKK95_09020"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOC18396.1"
                     /translation="MKKLKVLALVHRHLVPPEDTTGLDVINAEWKMEFDVKTTLEERG
                     HTVQVVGVHDDLGAIRRAVDEFQPAIIFNLLEAFADVTTFDQNVVSYLELLRRPYTGC
                     NPRGLTLARDKSLSKKLLAYHRVPVPEFTVVRRGRKPVLSKRMQFPLIVKSLVYESST
                     GISQASVVDGPAQLERRVQFVHESLGTAAIVEEFIDGRELYVGVIGNERLQSFPVWEM
                     FFSNMPDNARRIATERVKWNTKYQQRHGITTDMAVLDNGLGERIQHLARRAYRALDLS
                     GYARIDVRLDAGGRPYVIEANPNPQLAYGEDFAESAEKVGVSYEALLERIMALGLQWQ
                     PERTG"
     gene            complement(4630..5718)
                     /locus_tag="PKK95_09025"
     CDS             complement(4630..5718)
                     /locus_tag="PKK95_09025"
                     /inference="COORDINATES: protein motif:HMM:NF027219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HOC18397.1"
                     /translation="MQPDPPSDAPQEPDGVEAAAQQEPPVEWWALPDERLLDVRMCDL
                     PVSIEGSWIESRVDELRRELDGRGLQFPLTFYLSDEWFTPDGVSAIAIPFYVAHPRLH
                     KLEQSQMLEVEGGEHNWCMRILRHEAGHALDNAYGLRRRRLRRQIFGSPSEPYPEFYA
                     PRPYSRSFVLHLDSWYAQSHPDEDFAETFAVWLTPDNAWRSRYRGWPALKKLEYVDEL
                     MTALRGKPPSEVVTDPVDPLWKMRKTLRQHYRRKRRHYGLDRHRPAFYDRDLRRIFSD
                     APEFAHGISGAQFLARMRRPLRTLVASWTGIYQYTIDQVLEEMIQRCRELKLRLAMPE
                     DRARQELAVLLTVQSMKYLHSGGHRLAL"
     gene            5748..>8569
                     /gene="purL"
                     /locus_tag="PKK95_09030"
                     /gene_synonym="purI"
     CDS             5748..>8569
                     /gene="purL"
                     /locus_tag="PKK95_09030"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOC18398.1"
                     /translation="MQQILPIRGGSALSSFRLDKLNRQIEERGSRLRVAFATYWHFVE
                     TTRDPSPAERTVLERLLTYGWAGTEVRTHLEAVTAREGMRDVLVTPRPGTISPWSSKA
                     TDIARLCNLDFVRRIERGTVFHLEGDGDPADVIPVLHDRMTEAVLGSLDEAAQLFRHV
                     APKPLTEVDVLSRGPDAIVEANRRFGLALAPDEIDYLVDYFRRAGRNPTDVELTMFAQ
                     ANSEHCRHKIFNASWVVDGEPQADSLFRMIRRSHEANPQGTVSAYSDNAAVMEGAPIR
                     RFFADPDTGRYLFEQDLTHTLMKVETHNHPTAISPFPGAATGSGGEIRDEGATGTGSK
                     PKAGLTGFSVSNLRIPGYERPWEGPERRPDRIASPLSIMIEGPIGGASFNNEFGRPNL
                     AGYFRAYELEAGGRRRGYHKPIMLAGGVGNIRARHAHKQDVPAGSLLIQMGGPGMLIG
                     MGGGSASSMTTGSNTADLDFDSVQRGNAEIQRRAQEVIDRCWALGDRNPILSIHDVGA
                     GGLSNALPEIAHGAGRGARIDLRAAPNEEPGMTPREVWCNETQERYVLAIAPASLDLF
                     RRLCERERCPFAVVGEVTEERHLEVRDPLFGNKPVDMDLPALLGKPPRMTRDVRRLQP
                     ALPPLSLDGVTLAEALHRVLRAPSVADKTFLVTIGDRTVGGLCARDQMVGPWQVPVAD
                     CATTLLSFEGYAGEAFAIGERAPIAVIDAVASGRMAVGEALTNLAAAPVTDLSLVKLS
                     ANWMAAAGAPGEDAALFDTVRSVATDLCPALGVSIPVGKDSMSMRTAWEQDGRPEEVV
                     SPLSLIVSAFAPCADVRGTWTPQLRTDRGPTSLVLVDLAGGRTRLGGSVLAQVYCQAG
                     NEAPNLDDPAAIKAFYAALAELRAAGLVLAYHDRSDGGLLATLCEMAFAGHTGVTVEL
                     ARPAAGALAQLFAEELGAVLQVRDADL"
BASE COUNT         1353 a         3001 c         2936 g         1279 t
ORIGIN      
        1 accgaggccg aggcgaggaa gatcgccgcc gagagcaagc ccgtggtgtg catgaccatg
       61 agcatgcacg ccaccgagat cggcggggcg cagatgtcgc ccgagctggt ctacgacctc
      121 gtgacgcgcc aggacgccga gacgcgccgc atcctcgaca acgtggtgtt cctgctggtg
      181 ccgtcgttca accccgacgg ccagatcatg gtgaccgact actacaacaa gtacctcggc
      241 accgagtacg aagccggcag cctgccgtgg ctctaccaca agtacgtcgg ccacgacaac
      301 aaccgcgacg cgatgacgat gaacatgaag gagtcgcagt acgtcggccg gctcctgttc
      361 accgagtgga agccgcaggc ctatttcgac ttccaccaca tgggcagcta cggcgcgcgg
      421 atgtactttc cgccctacgc ggagccgatc cgcccgcttg ccgacccgct ggtctggacc
      481 gagtcggcgt ggtacggggg ccagatggcc aacaaggcct ccgaggcggg gctcacgggc
      541 gtgctgaaca acgggcagta ctccgggtgg gggcacttcg gcttccactg gatcacgccg
      601 ttccacaaca tcgccggcat gctcagcgaa gccgccagcg ccaggctggc gacgccgctg
      661 tacatcgacc cgagccagct gaccggacga tcgcggaacc agcaggaccc ccgcgagccc
      721 cagatgaact ttcccgatcc gtggcccggc ggctggtgga ggctgcgcga tatcgtggac
      781 cggcagaagg tgggcgcctg ggccctgctc gactgcatgg cgcgcaacgc cgagacgatt
      841 gtctgggaca cctacgtcaa ggcgatgcgc cagacagagc gaggcgcgaa gggaaagccc
      901 gccgcctacg cgatctcggc cgaccagcac gatccgctca cgctcgacaa gctcgtcaac
      961 gccctgcttg cccagggcat cgaggtcctg cgctcggcga agggtttcgc ggcgccgggc
     1021 ggcgtcgtct atccggccgg ctccttctac gtcacgctcg cgcagccgaa gatgggactc
     1081 gttcgctacc tgctcggcga aacgcactac cccgagaacg agtggacgcg gcagcaggac
     1141 ggcacaccca tccggcccta cgacatgggg caggacgtcc tcgccgaatt catgggaatc
     1201 agggtcgatc ctctcgacga ggcggtcgag gccgacttca ccagggtggc ggccccgatc
     1261 ctccgtgcgg gcaccgtcga gaaggggccg gcggggtacg tcatcgacgg ccggctgaac
     1321 gacgcgttca gggcggtgaa cctgttgttc gaccgtgacg tcacggtgcg ccgcgccgac
     1381 gccgccggcg acgccgtgcg cgcgggcgac ttcctgttgc ccgcctcggc ccccgccgcc
     1441 gtgctcgccg aggtggcgaa gacgaccggc gtggaccttc gcgccctgaa ggccgaacca
     1501 aaggccgccc acgagctgaa gcgtcttcgc atcggcatgt accagcgcta ccgcggcggc
     1561 aacatggacg aggggtggac gcgctggatc ttcgagcagt tcgggttcca gtacaagagc
     1621 gtgctggacg gcgagatcaa gaagggcaac cttcgggcaa gctacgacgt gttcgtcttc
     1681 gcagacgact ccacctcggc gattaccggc gagcctgcgc cgtcccggcc gggcggcttc
     1741 tcccgcggcg cgcggttcga gggagtgccc gatacgacgc cgcccgagta caagagcggc
     1801 atcggcgccg acggtgtggc ggcgatcaag gagttcgtgc gcgccggtgg gacgctggtc
     1861 accctcggag gcgcgacaga cttcgcgatc tcgaagctgg gactgccggt gaggaacgtc
     1921 gtcgagggcc tgagcaccaa ggagttcttc tgtccgggtt cgacgctgcg ggtgacgatc
     1981 gacaacgccc accccctggc gtacggcatg ccggcggaag ggttggtgct gttccactac
     2041 agcccggcgt tcgacgtggt cgccaccgac ttcaaccacc gcaacgacgt cgtcgtccgc
     2101 tacgccgagc gcgacgtcga gcggagtggc tggctgaacg gcgaagagca catcgccggg
     2161 aagaccgcga tggcgtcgat cggctacggg gagggccggg tggttctaat tggcttccgc
     2221 acgcagaacc gcgcgcagac gctcggcacc tacaagttcc tgttcaacgc gctggtcacg
     2281 ccgccgcccg cagcgcgcaa tcagccgtag cgcagggctc gtagcgcagc gtcgtaacgc
     2341 agggcttcag ccctgcggaa gcaccacccg tagcgcaggg cttcagccct gcggaacact
     2401 ccgaggagca cggtatggca accggcatcg agcgcaagac ctgccaggcg cctgacggcg
     2461 tcaccatcgg gtattcggtt gtcgggaagg gcgagccggc cctgctgttc gttcacggcg
     2521 ccatggccga ccgctcgttc tacgactcgc agctgcaggc cctgtcggat cgttggcgcg
     2581 tcatcgccgt agacctggcg ggccacggcg aatcgggtgt gaaccgcacg acctggggaa
     2641 ttcgcgagtt cggggccgac gtcaaggcgg tggcggacgc cgagggcctg aagcgcctcg
     2701 tcgtcttcgg caactctctg gggggaccgg tggccgtcga ggcggcgctg ctcctgcccg
     2761 gccgcgcggt gggcgtggtg ggcatcgaca cgttccagga ccttggccag gcagcctcgc
     2821 tcgaataccg gcgcgcggcg gacgagtact atcacaagcg cgccgagtcg ttccgagccg
     2881 actacccggt ggccttgcgc gagatggtga ggatgctgtt ccacccggat gccgatcccg
     2941 acctcgtcgc ggaggtcgag cggcgcatgc tgaggacgcc cgcgcccgtg gccgctgcca
     3001 cgctcgacag cctgccgggt tacgaccagg aagccgccgt ccgcaagttg aacgttccgc
     3061 tgcgctcgat caccggcgac ctgttcccca cagacctgca gaaggcacgc gccgtgacgc
     3121 ccgatttcga cgtcatcgtc atgaagcaca ttggccacta cccgatgctc gagcggcccg
     3181 gtgagttcaa ccggcacgtc gcggagatgg tcgcccggct ggcacggtag cgtcggccac
     3241 cgcgcgagcc caacccatcg cgaccgcttc aggtatcccc cttgctttcg ctcagcctgc
     3301 gccaggccgt gagcgggccg gccccagcga aagggacggg cgtgcctgct cgacctggtc
     3361 gccgcgcagg tcgggcgggc gcccggcgcc gtggcgatcg agtcgagcct cggccgcgtc
     3421 acctgcggag gccgcctcac gaacggccgc ctcgcctcag gaaaatcatc aggaacgcga
     3481 tgaggagccc ccccgcggcc gccaccgtca ggacgccggc gaacggcgag aagcttcgcg
     3541 tgaccaggta cgcgaagccc gcgaagagaa cgaggatggc ggcccgaacg agaagcatcg
     3601 cagtctcccc cctctatccg gtccgctcag gctgccactg caggccgagg gccatgatcc
     3661 gctcgagcag cgcctcgtac gaaaccccga ccttctcggc cgactcggcg aagtcctccc
     3721 cgtaggcgag ttgcgggttc gggtttgcct cgatgacgta gggccgcccc ccggcatcca
     3781 gtcgcacgtc gatccgcgcg tagccgctga gatcgagcgc ccggtacgcg cggcgcgcca
     3841 ggtgctggat ccgctcgccc agcccgttgt cgagcaccgc catgtcggtg gtaatcccgt
     3901 ggcgctgctg gtacttcgtg ttccacttca cccgctcggt cgcgatccgc cgcgcgttgt
     3961 cgggcatgtt cgagaagaac atctcccaca ccggaaacga ctgcagccgc tcgttgccga
     4021 tcaccccgac gtacagctcg cgcccgtcga tgaactcctc gacgatggcg gcggtcccga
     4081 ggctctcgtg cacgaactgg acccgccgct cgagctgcgc cgggccgtcc accaccgagg
     4141 cctgcgagat gccggtggac gactcgtaca cgagcgactt gacgatgagc gggaactgca
     4201 tccgcttcga cagcacgggc ttccgtcccc gccgcacgac ggtgaactcg gggaccggca
     4261 cgcggtggta cgcgagcagc ttcttcgaaa gcgacttgtc gcgcgccagg gtcagccctc
     4321 tcggattgca gccggtgtag ggccgccgca gcagctccag gtagctgacg acgttctggt
     4381 cgaaggtggt gacgtcggcg aaggcctcga gcaggttgaa gatgatggcg ggctggaact
     4441 cgtccaccgc gcgccggatc gcgccgaggt cgtcgtgcac tccgaccacc tgcacggtgt
     4501 gcccgcgctc ctcgagcgtc gtcttgacgt cgaactccat cttccactcg gcgttgatca
     4561 cgtcgaggcc ggtcgtatcc tccggcggga cgaggtgccg gtggacgagc gcgaggacct
     4621 tcagcttctt catagggcaa gccggtggcc gccgctgtgg aggtacttca tcgattgcac
     4681 cgtgaggagg accgccagct cctgccgggc ccggtcctcg ggcatcgcca ggcgcagctt
     4741 cagctcgcgg cagcgctgga tcatctcctc gagcacctgg tcgatggtgt attgatagat
     4801 gccggtccag ctggcgacca gcgtccgcaa cggccgccgc atccgggcca ggaactgcgc
     4861 cccgctgatc ccgtgggcga actcgggggc gtccgagaag atgcggcgga ggtcgcgatc
     4921 gtagaacgcc gggcggtgcc tgtcgaggcc gtagtgccgc cgcttgcgcc ggtagtgctg
     4981 gcgcagcgtc ttcctcatct tccagagcgg gtccaccgga tcggtcacca cctcggaagg
     5041 gggcttcccc ctcagcgccg tcatcagctc gtcgacgtac tccagcttct tgagcgcggg
     5101 ccagccccgg taccgcgacc gccacgcgtt gtcgggcgtc agccagacgg cgaaggtctc
     5161 ggcgaagtcc tcgtcagggt ggctctgcgc gtaccacgaa tcgaggtgca ggacgaagct
     5221 ccggctgtag ggccgcggcg cgtagaactc ggggtaaggc tcggagggcg agccgaagat
     5281 ctggcggcgc aggcgtcgcc gccgaagccc gtaggcgttg tcgagggcgt gcccggcctc
     5341 gtggcgcagg atgcgcatgc accagttgtg ctctcccccc tcgacttcga gcatctgcga
     5401 ctgctcgagc ttgtgaagcc tcgggtgcgc gacgtagaag gggatcgcaa tcgccgacac
     5461 gccgtcgggc gtgaaccact cgtctgacag gtagaaggtc aggggaaatt gcaggccgcg
     5521 cccgtccagc tcgcgccgaa gctcgtccac ccgcgactcg atccaggacc cctcgatgct
     5581 gacgggaaga tcgcacatcc tcacatcgag caggcgctcg tcgggcagcg cccaccattc
     5641 gacgggcggc tcctgttggg ctgctgcctc gacgccgtcc ggttcttgcg gcgcgtcgga
     5701 aggggggtcg ggctgcatgc gcacatgata ccgggtacaa ttcacgcatg cagcagatcc
     5761 tcccgattcg cgggggttcc gcgctctcat ccttcagact cgacaagctg aaccggcaga
     5821 tcgaagagcg cggcagccgt cttcgcgtgg ccttcgccac ctattggcat ttcgtcgaaa
     5881 ccactcggga tccctcgccg gccgagcgca ccgtcctcga gcgcctcctg acctacgggt
     5941 gggcgggcac ggaagtccgc acgcacctag aggccgtgac cgcgcgggaa ggcatgcgcg
     6001 acgtgcttgt aaccccgcgt ccgggcacga tttcgccctg gtcgtcgaaa gccaccgaca
     6061 tcgcccggct ctgcaacctc gatttcgttc gccgcatcga gcgcggcacc gtcttccacc
     6121 tggagggcga cggcgacccg gccgacgtga tcccggtgct gcacgaccgg atgaccgagg
     6181 cggtcctcgg ctcgctcgac gaggcggcgc agctcttccg ccacgtcgcc ccgaagccgc
     6241 tgaccgaggt ggacgtcctc tcgcgcggcc ccgacgcgat cgtcgaggcg aatcggcgct
     6301 tcggcctcgc gctggcgccc gacgagattg actacctcgt ggactacttc cgccgcgccg
     6361 ggcgcaaccc caccgacgtc gagctgacga tgttcgcgca ggcgaactcg gagcactgcc
     6421 gccacaagat cttcaacgcg tcgtgggtgg tggacggcga gccgcaggcc gactcgctgt
     6481 tccggatgat ccgccgctcg cacgaggcga acccgcaggg gaccgtgtcg gcctactcgg
     6541 acaacgcggc ggtgatggag ggggcaccga tccgccgttt cttcgccgac cccgacaccg
     6601 ggcgctacct cttcgagcaa gacctcaccc acacgctgat gaaggtcgaa acgcacaacc
     6661 acccgaccgc gatctcgccc ttccccggcg cggccaccgg gtcgggcggc gagattcgcg
     6721 acgagggagc caccggcacc ggctcgaagc ccaaggccgg cctcaccggc ttctcggtgt
     6781 ccaacctgcg gatcccggga tacgagcgcc cgtgggaagg ccccgagcgg cggcccgacc
     6841 gcatcgcctc gccgctctcg atcatgatcg agggacccat tggcggcgcc tcgttcaaca
     6901 acgagttcgg gcggcccaac ctggccgggt acttccgggc gtacgagctg gaggccggcg
     6961 ggcggcggcg cggctaccac aagccgatca tgctggcggg aggggtcggg aacatccggg
     7021 cgcggcacgc gcacaagcag gacgtgccgg ccggctcgct gctgatccag atgggcgggc
     7081 cggggatgct gatcgggatg ggcggcggtt cggcctcgtc gatgacgacc ggcagcaaca
     7141 ccgccgacct cgacttcgac tcggtccagc gcggcaacgc cgagatccag cggcgtgccc
     7201 aggaggtgat cgaccgctgc tgggcgctcg gcgaccgcaa ccccatcctc tcgatccacg
     7261 acgtcggtgc cggcggcctc tccaacgccc ttcccgagat cgcgcacggc gcggggcgcg
     7321 gcgcccggat cgacctgcgc gcggccccga acgaggagcc gggcatgacg ccgcgcgagg
     7381 tgtggtgcaa cgagacccag gaacgctacg ttctcgccat cgcccccgcg tcactcgacc
     7441 tgttccggcg gctctgcgaa cgcgagcgct gccccttcgc ggtagtcggc gaggtaaccg
     7501 aggagcgtca cctcgaggtc cgcgacccgc tgttcggcaa caagcccgtg gacatggacc
     7561 tgccggcgct gctcggcaag ccgccgcgca tgacgcgcga cgtgcggcgc ctgcagccgg
     7621 cgctgccgcc cctgtcgctc gacggcgtga ccctcgccga ggcgcttcac cgcgtgctgc
     7681 gggcgccctc ggtcgccgac aagaccttcc tcgtcaccat cggcgaccgg accgtcggcg
     7741 gcctctgcgc ccgcgaccag atggtgggac cctggcaggt cccggtggcg gactgcgcga
     7801 cgaccctcct gtcgttcgag gggtacgcgg gcgaggcgtt cgccattggc gagcgggcac
     7861 cgatcgccgt gattgacgcc gtggcgtcag ggcggatggc cgtcggcgag gcgctcacca
     7921 acctggccgc cgcgccggtg accgacctgt cgctggtgaa gctgtcggcc aactggatgg
     7981 cggccgccgg ggcgcccggc gaggacgcgg ccctgttcga cacggtcagg agcgtggcga
     8041 ccgacctctg tcccgcgctg ggcgtcagca tcccggtcgg caaggactcg atgtcgatgc
     8101 ggaccgcgtg ggagcaggac ggccgccccg aggaggtggt ctcgccgctc tcgctgatcg
     8161 tctcggcctt cgccccctgc gccgacgtgc gcgggacctg gacgccgcaa ctgcggaccg
     8221 accggggccc gacctcgctc gtcctggtcg acctggcggg cggccggaca cggctgggcg
     8281 gctcggtgct cgcacaggtc tactgccagg caggcaacga agccccgaac ctggacgatc
     8341 ccgccgcgat caaggccttc tacgccgcgc tggctgagct tcgcgccgcc ggcctcgtgc
     8401 tcgcctacca cgaccggtcg gacggcggcc tgctggcgac gctctgcgag atggcgtttg
     8461 ccggccacac cggggtcacc gtcgagctgg ccaggccggc ggccggcgcg ctcgcccagc
     8521 tcttcgccga ggaattgggg gcggtgctgc aggtgcggga cgccgacct
//
LOCUS       DAOPQX010000143         8563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9234_length_8563_cov_5.154678, whole genome shotgun sequence.
ACCESSION   DAOPQX010000143 DAOPQX010000000
VERSION     DAOPQX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9234_length_8563_cov_5.154678"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1158)
                     /locus_tag="PKK95_09035"
     CDS             complement(268..1158)
                     /locus_tag="PKK95_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18399.1"
                     /translation="MSLQTLLLVALQASVFLTVLTIGMGTSGADLRYVLGNPRRLIRS
                     LLALNVLGPIVAVVVCQLFSLHPAVIVALVTLAIAPVSNMFPKAMLPLVGPGRAAAYA
                     HGLFFASTVLSVILTPLAVEVINAIFGGREHVSPLAVAEVVVASVLLPLGIGVAIGTR
                     WQAAKAWIPAIQKVSGLVLLVCAVVIVAAVWSLMGSLIRQGTLSAIAIIALIGLVAGH
                     WLGGPDEDDRTVLAEATVSRHPGVAIAVASLTDQPLAPVGVLLAVLVSAIAVVPYTQW
                     RKRLRGAGPARAGHAPSRAH"
     gene            complement(1237..2931)
                     /locus_tag="PKK95_09040"
     CDS             complement(1237..2931)
                     /locus_tag="PKK95_09040"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOC18400.1"
                     /translation="MERHAVTGGRDDRQSAAPTLRIAWAWAVVLLVALAGPAGAAGQT
                     GPEPSRTPPPPEEAGDPLGRDTPRGTIAGFNLAVHREDFQSAARFLQLSAGGRRDTER
                     LARDLADLIDRYYIQPLAALSDDPQGTATDGLALDRERVILTMAGKPVDLELVRVKDR
                     EAGLVWLVSAQTLARVPALRRSAGVTWLEGLMPAALVDARLFGLSIAQWVAWAATLLV
                     PAAALWLLSAIVIASARAVSGPKRRALIDTWYGGLRWPVILVVTLAVHVAAVPLLGFS
                     LAFRFGYSRLELAVVVLAVAWFLWRLMALSFTHARIMALRRGQASVRSLLLLAERVSK
                     TIVALAAVFALLTIAGVDTTTALAGVGIGGVAIALGAQRSVENLLGGVFLLTDRALAV
                     GDTCSIADRLGVVEDITMRSVRLRTLEQTLLSVPAGVLAQSSVENFATRDKILVLSTL
                     RLRYDTTAAQLRQVLGRIRGLLEERPEIEKETARIRLVDFGVYAIELELFAYLLTPDF
                     SKFLALREELLLQVAAIVESSGSGFARPEIDYGISAAEATSARFGGPNRGTANRSV"
     gene            complement(2959..5145)
                     /locus_tag="PKK95_09045"
     CDS             complement(2959..5145)
                     /locus_tag="PKK95_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOC18401.1"
                     /translation="MAHGQQTGTATTTVVERDAALDARAREVEQQMTDDERFSLVVSV
                     FGHVPGGLAGPRDARIPQAVKNMSAGYTPGVPRLGIPAIQSSDASMGITNPGYRPDDA
                     GATAFPSLIALGASFNPGLVREGGEAIGREARIRGFNVMLAGGCNLARDPRNGRNFEY
                     FSEDPYLSAVLAAAQVNGIQSQGVVSTLKHYTLNCNETNRHWLDAIVDPDAHRESDLL
                     TFQVAIEQSQPGSIMTGYNKINGDYAGGNHRLLNEVLKDAWGYKGWVMSDWGATPEWE
                     FALRGLDQESGVQADRVMWGKEPFTDDLRKAYAAGKLPKERLSDMVRRILRSLFAVGA
                     ESWGPGPGPEVDMARHDEIALKGARQGIVLLKNAGALPLPADKPLKVAVIGGFAQQGV
                     ISGTGSGAVAPVGGFAGVVRIGGAGVMGKHRNLFLFAPSPLEELKKAMPKAQFDFDPG
                     YTPAEAALTAKRADVVIAFGIRVEGEGFDSVDLSLPWGQDAVIEAVAAANPNTVVVLE
                     TGNPVSMPWQNKVKAIVQAWFPGQAGARAIADVLTGKVNPSGRLPISFPESLAQTPRP
                     ELPGLGTPWGTAVTIRYDEGAEVGYRWYAQKNHKPLYPFGHGLSYTTFGYSDLKVEGG
                     DTVTATFTVTNTGKVAGADVPQLYLTNAAGDKRVRLLGFERVELKPGESRRVTITADP
                     RLLARFDGSIGQWRLNEGAYAVALSRSATEPVARDETHVTGRVFGH"
     gene            complement(5181..6386)
                     /locus_tag="PKK95_09050"
     CDS             complement(5181..6386)
                     /locus_tag="PKK95_09050"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOC18402.1"
                     /translation="MALSLAWAVVPANAQEPPASRDGRAGTQQAAGQSRTTWQASWTQ
                     FLGAAVAGIGGDDAKFGGKLTGSVAIDGHDVRLWDGFSINAGAEFVFGGSVNGSGTGT
                     ILPLNIAMMFPASGKEDIDFSLNFTQRFGGSSLTVGRINMLDTVVRTPLISGGGLEGF
                     QHAALAAPPSELTPPWIFGAIWSRPSPRAVVTVGAWDVRSALNRALVDSLFADGIAGM
                     TSVTFPIRAGGQPGFHGLTVMATSKRGLNLEDIGDLLLPPESERVLGQKRGGWFVKYA
                     FQQFLWHDAANPSRGWGLFGHIGTWDANPTPMQWSMTLGLGGSPPLDSRPRDRFGVGF
                     FRTSLSETLRDGLDPILDLDDEQGLEAFYTLEAIPHLRITGTVQWVDPAVRGIRNAVV
                     AGLRTRVAF"
     gene            6918..7376
                     /locus_tag="PKK95_09055"
     CDS             6918..7376
                     /locus_tag="PKK95_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18403.1"
                     /translation="MICEDPESAHRVVADYARLLERGDEQPLPAPVRLLPHPKPIIKA
                     AILTCASALGRDGRLTAGMRDFLEQAYVALADYVDDDLVRVMTEYRQSLDEIAGLPGA
                     REKQQTPAWRRIVETGRLAGEIARSIADEGASLRLEFGARVASPSKENPG"
     gene            7373..>8563
                     /locus_tag="PKK95_09060"
     CDS             7373..>8563
                     /locus_tag="PKK95_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18404.1"
                     /translation="MTPSVPGHVEQEVEQETVRLTLKSRREIEEEIARRVAGERERLI
                     GLAAPRAGVAHFRRPAERPFTAAERDAVTILIGGLTWKHEQLIRAVFQSAGYQVEILP
                     TPDVQSFQLGKEYGNNGQCNPTYFMVGHLIKFLQGLEAAGMTRQEIIDRYVFFTAGSC
                     GPCRFGMYESEYRLGVQNAGFDGFRILLFQQNDGIKAASGEPGLKFTVDFGLGALSAL
                     NLGDIVNDVTYQIRPYEMVPGETDRALAEVMEDLSARMRDRRMFEAMDRLPRWLASRV
                     ASRRALKITVSVVGKIADHWYGRQTREALAAAREQLSRIEVDRLRVKPLVKVTGEFFA
                     QTTEGDGNFRMFSFLEREGAQVQVEPIGNWIMYLLYQARERYLQQRRVSTGKLPAWQL
                     RRRAA"
BASE COUNT         1347 a         3014 c         2936 g         1266 t
ORIGIN      
        1 aacggcagct ggcttctgct gctgctgctg cggcggcggc ggcggcggct gctgctgcgg
       61 aatcgccagt tgcgggacga ggaagagcag cagggaacca gtggcgatgc gaatcgccgc
      121 cgtcgtgcgc gcggatgcct tccttttctt cataccgttg cactcctggg gcgcggtcga
      181 tcaggcgccc cggctgaggc cgccggcgtg gcgtcggcct ggcggccgtc ccctcgtcga
      241 acgtgcagga gtcggcggca cgtgccttca gtgggcgcgt gagggcgcgt gcccggcgcg
      301 cgccggcccc gctccgcgca accgctttcg ccactgcgtg tacggcacga cggcgatggc
      361 gctcaccagc accgcgagaa gcacgccgac cggcgcgagc ggctgatccg tgaggctcgc
      421 gaccgcgatc gcgacccccg gatgacgtga gacggtcgcc tccgcgagca cggtgcggtc
      481 gtcctcgtcg ggaccgccga gccagtgccc cgcgacaagg ccgatcaggg cgatgatcgc
      541 gatcgcgctc agcgtcccct ggcgaatcaa cgaacccatg agcgaccaga ctgccgcgac
      601 gatgaccacg gcgcacacca ggagcaccag gccgctcacc ttctggatcg ccggaatcca
      661 tgccttggcg gcttgccacc gcgtgccgat ggcgacgccg atgccgagcg gcaggagcac
      721 gctggcgacg acgacctccg ccacggcgag cgggctcacg tgctccctcc cgccgaagat
      781 cgcgttgatc acctcgacgg cgagcggcgt gaggatcacc gagagcacgg tactcgcgaa
      841 gaacagcccg tgcgcgtagg cggcggcgcg gccgggcccg acgagcggca gcatggcctt
      901 gggaaacatg ttgctcacgg gggcgatggc cagcgtcacg agcgccacga tcacggccgg
      961 gtgcagcgag aacagctggc acaccaccac ggcgacgatc ggcccgagga cgttcagcgc
     1021 gagcagcgac cggatcagcc gcctcgggtt gccgaggacg tatcggaggt cggcgcccga
     1081 cgtccccatg ccgatcgtca gcacggtgag gaagacgctc gcctggagcg ccacgaggag
     1141 aagtgtctgg aggctcatcc cgtttctccc gcttcacgcg cgaagcgctg gctgcgtccc
     1201 ggccccggtc gaatgggatc tcccgcttgc cgtctctcac accgaccggt tcgcggtgcc
     1261 ccggttcggc ccgccgaacc gggcactcgt cgcctcggca gccgaaatcc cgtagtcgat
     1321 ctcgggccgc gcgaagccgc tgcccgacga ttcgacgatg gcagccacct ggagcagcaa
     1381 ttcctcgcgc agggcgagga acttcgagaa gtccggcgtc aggaggtagg cgaagagttc
     1441 cagctcgatc gcgtacacgc cgaagtccac gagccggatc cgggccgtct ccttttcgat
     1501 ctccgggcgc tcctccagga gccctcgaat gcggcccagc acctgcctga gttgagcggc
     1561 ggtcgtgtcg taccggagcc gcagggtgct cagcacgagg atcttgtcgc gcgtcgcgaa
     1621 gttctccacg ctcgactgcg ccaggacgcc cgccggcacc gacagcagcg tctgctcgag
     1681 ggtcctcagg cgcaccgagc gcatcgtgat gtcctccacg acgcccagcc ggtcggcaat
     1741 gctgcaggtg tcgccgaccg ccagcgcgcg gtcggtgagc aggaacacgc ccccgaggag
     1801 gttctccacg ctccgctgcg cgccgagcgc gatggccacg ccgccgatgc cgacgccggc
     1861 cagggcggtg gtggtgtcca cgcccgcgat cgtgaggagc gcgaagaccg ccgcgagggc
     1921 gacgatcgtc ttcgagacgc gctcggccaa caggagcaac gagcgcacac tcgcctgccc
     1981 cctgcgcagc gccatgattc gggcgtgcgt gaacgagagc gccatgagcc gccacaggaa
     2041 ccacgctacg gcgagcacca ccacggccag ctcgaggcgg ctgtatccga aacggaacgc
     2101 cagcgagaac cccaggagcg ggaccgccgc gacgtgaacg gccagcgtga cgacgaggat
     2161 caccggccac cgcaggcccc cgtaccacgt gtcgatcagc gcgcgccgct tcggcccgct
     2221 gacggcccgg gccgaggcga tcacgatcgc cgacagcagc cacaacgccg ccgccgggac
     2281 caggagcgtc gccgcccatg ccacccactg cgcgatcgag aggccgaaca gcctggcatc
     2341 gacgagcgcc gccggcatca ggccctcgag ccacgtcacg cccgccgatc gccgcagcgc
     2401 cggcacgcgc gcgagggtct gcgcggacac gagccagacg agcccggcct ctcgatcctt
     2461 gacgcgcacc agctcgagat cgaccggctt gccggccatc gtgaggatca cgcgttcgcg
     2521 gtccagcgcg agaccgtcgg tggcggtgcc ctgcgggtcg tcactgagcg ccgcgagcgg
     2581 ctggatgtag taccgatcga tcaggtcggc gaggtcgcgc gcgagccgtt ccgtgtcgcg
     2641 gcggccgccc gccgaaagtt ggaggaaccg ggccgcggat tggaagtcct cgcgatgcac
     2701 ggcgaggttg aagccggcga tcgtgcctct cggggtgtcg cggcccagcg gatcgccggc
     2761 ttcctcgggc gggggtgggg tgcgggaggg ctcgggaccg gtctgccccg ccgctccggc
     2821 gggccccgcc agggcgacga ggagcacgac ggcccacgcc cacgcaatcc gcagcgtcgg
     2881 cgcggcactc tgccgatcgt cccggccgcc ggtgacggcg tgacgttcca tgcggatctc
     2941 ccgaaccccg atcgtcgttc aatggccgaa cacgcggccg gtcacgtgcg tttcgtctct
     3001 ggcaaccggc tcggtggccg aacgtgacag cgcgacggca tacgcgccct cattgagacg
     3061 ccactgcccg atcgacccgt cgaaccgcgc gagcagtctc gggtcggcgg tgatggtgac
     3121 gcgccgcgac tcgcccggct tcaactcgac gcgctcgaac ccgagcagcc gcacgcgctt
     3181 gtcgccggcc gcgttcgtca ggtacagctg cggcacgtcg gcgccggcga ccttgcccgt
     3241 gttggtgacg gtgaaggtcg cggtgaccgt gtcgccgccc tcgaccttca ggtcgctgta
     3301 cccgaaggtc gtgtagctca ggccgtgccc gaacgggtag agcggcttgt ggttcttctg
     3361 cgcgtaccag cgatagccga cctccgcgcc ttcgtcgtag cggatggtca ccgccgtccc
     3421 ccacggggtg cccaagccgg ggagttcggg acggggtgtt tgcgcgaggc tttcagggaa
     3481 cgagatcggc agccgccccg acgggttcac cttgccggtg agcacgtccg cgatcgcccg
     3541 ggcgccggcc tggccgggga accacgcctg cacgatcgcc ttgaccttgt tctgccacgg
     3601 catcgacacc gggttgccgg tctccagcac cacgaccgtg ttggggttgg cggcggcgac
     3661 ggcctcgatc acggcgtcct gcccccacgg cagcgacagg tccacgctgt cgaacccctc
     3721 gccttcgacg cgaatcccga aggcgatgac cacgtcggcg cgcttggccg tcaacgccgc
     3781 ctccgccggc gtgtacccgg ggtcgaagtc gaactgcgcc ttcggcatcg ccttcttcag
     3841 ctcctcgagc ggagagggcg cgaagaggaa gaggttccgg tgcttcccca tgacgcccgc
     3901 accaccgatc ctgacgacgc cggcgaagcc acccacgggg gccaccgcgc ccgagccggt
     3961 gccgctgatc accccttgct gggcaaaacc tccgatgacc gcgaccttga gcggtttgtc
     4021 ggccgggagc ggcagcgcgc cggcgttctt caggagcacg atcccctgcc gggcgccttt
     4081 cagggcgatc tcgtcgtgcc tcgccatgtc cacctcaggc cctggtcctg gtccccagct
     4141 ttccgcacca accgcaaaca aggagcgcag gatgcggcgg accatgtcgg agagccgctc
     4201 cttcggcagc ttgccggccg cgtacgcctt ccggaggtcg tcggtgaagg gctccttgcc
     4261 ccacatcacc ctgtcggcct gcacacccga ctcctggtcg agccccctga gcgcgaactc
     4321 ccattccggc gtcgcgcccc agtcggacat cacccagccc ttgtagcccc acgcgtcctt
     4381 gagcacctcg ttcaggaggc ggtggttccc gccggcgtag tcgccgttga tcttgttgta
     4441 gccggtcatg atcgagcccg gctgcgattg ttcgatggcg acctggaagg tgagcaggtc
     4501 agactcgcgg tgggcgtcgg ggtcgacgat cgcgtcgagc cagtggcggt tcgtctcgtt
     4561 gcagttgagc gtgtagtgct tgagggtgga gacgacaccc tggctctgga tcccgttcac
     4621 ctgcgcggcg gcgagcacgg cgctgaggta gggatcctcg gagaagtact cgaagttccg
     4681 gccgttgcgc gggtcgcgcg ccaggttgca cccgccggcc agcatgacgt tgaagccccg
     4741 gatccgcgcc tcgcggccga tcgcctcgcc cccttctcgc acgagcccgg ggttgaagct
     4801 cgctccaagg gcaatcagcg aagggaacgc ggtcgcgccc gcgtcgtccg gccggtagcc
     4861 gggattggtg atccccatgc tggcgtcgct gctctggatg gccgggatgc cgagccgggg
     4921 aaccccggga gtgtagcccg cgctcatgtt cttgacggct tgggggatgc gcgcgtcgcg
     4981 cggcccggca aggcctcccg ggacatggcc gaacacactg acgacgagcg agaaccgctc
     5041 gtcgtcggtc atctgctgct cgacctctcg cgcgcgggca tcgagcgccg cgtcacgctc
     5101 gacgactgtc gtggtggccg tgccggtttg ctgcccgtga gccactggta acctcctcat
     5161 ttctttctgg tcgcgggacg tcagaacgcg acgcgcgtcc ggagtcccgc caccaccgcg
     5221 ttgcggatgc cgcgaacggc gggatccacc cactggaccg tgccggtgat ccgcaggtgg
     5281 ggaatcgcct cgagggtgta gaacgcctcg agcccctgct cgtcgtcgag atcgaggatc
     5341 ggatcgaggc cgtcgcgcag ggtctcgctc aaggaggtcc ggaagaaccc caccccgaaa
     5401 cgatcacggg gccggctgtc gagcggcggc gagcccccaa ggcccagggt catgctccac
     5461 tgcatgggcg tggggttcgc gtcccacgtc ccgatatgac caaacaggcc ccagccccgc
     5521 gaggggttcg ccgcgtcgtg ccagaggaac tgctggaagg catacttcac gaaccagccg
     5581 ccgcgcttct gtccaaggac ccgctcgctc tcgggcggca gcagcaggtc gccgatgtcc
     5641 tcgaggttca ggccgcgctt gctggtcgcc atcaccgtga ggccgtggaa tcccggctgg
     5701 ccgcctgccc ggatcgggaa ggtgacgctg gtcatcccgg cgatcccgtc ggcgaacagc
     5761 gagtcgacca gcgcgcggtt gagcgcgctc ctgacgtccc acgctccaac ggtgacgacc
     5821 gctcgcggcg acggacgcga ccagatcgcc ccgaagatcc acggcggggt gagttcactt
     5881 ggaggggcgg caagggcggc gtgctggaag ccctcgagcc cgccgccgct gatgagcggc
     5941 gttcggacaa cggtgtcgag catgttgatc cgcccgaccg tcagcgacga gccgccgaac
     6001 cgctgcgtga agttgagcga gaagtcgatg tcttccttgc cgctggcggg gaacatcatc
     6061 gcgatgttga gtgggaggat cgtcccggtg ccgctcccgt tcacgctgcc gccgaacacg
     6121 aactccgcgc cggcgttgat tgagaagccg tcccacagcc ggacgtcgtg cccgtcgatt
     6181 gccaccgaac cggtcaactt gccgccgaac ttcgcgtcgt ccccgccgat ccccgcgacg
     6241 gcggcgccga ggaactgcgt ccaggacgct tgccacgtgg tcctcgactg accggcggcc
     6301 tgctgcgtcc cggcccggcc gtcccttgac gctgggggct cctgggcgtt cgcgggcacc
     6361 actgcccagg ccaacgacag ggccatccca agcatcgcgg acctgcacgt agcggccggc
     6421 ctcttatgac aattcggcac cgctcctctc ctgtgctgca ttcgcggtcc acagtcaccg
     6481 cggagacacg aggaccaagc ggaggcctct gcggtgaacc cgtcgagtcg tcagtgagcg
     6541 ccgcgattgg agatacaaga agagcgccgc tgagagtcgc tcgaatctgc gggaatgcgc
     6601 ggacgaatct gaagggaatc tgcagggatg cgcggaggac ccagtcaagg acagcagtac
     6661 tagcaggagt gttcgtgccg cgaagctcgg taggcggaca tgactgtgta acctgagacg
     6721 acacatcagc cggcccagcg gctccagccc tgccgtgcct catcggcttc agacggcctc
     6781 tgcaactgcg aacgttcgag cgggaattcg cgcagaccgc cctgaacccc gcccgacggc
     6841 cgatagtccg cggccggttc gccccttgca cgtcgcagcg cggcccggcc gtggatcggc
     6901 cggcccttca attgacgatg atttgcgagg accccgagtc ggcccaccgc gtggtcgccg
     6961 actacgcccg tcttctcgag cggggcgacg agcagccgct gcccgcgcca gtgaggctgc
     7021 ttccccatcc caagccgatc atcaaggcgg ccatcctgac gtgcgcctcg gcgctcggcc
     7081 gtgacggccg cctcaccgcc ggcatgcgcg atttcctcga acaggcgtac gtcgcgctcg
     7141 ccgactacgt ggacgacgac ctggttcgcg tgatgaccga gtaccggcag tcgctcgacg
     7201 agattgccgg cctgccaggc gcgcgcgaaa agcagcagac gccggcgtgg cggcgcatcg
     7261 tcgagacggg ccgcctggcg ggcgagatcg ccaggagcat cgcagacgag ggcgcctcgc
     7321 tgaggctcga gttcggggcc cgcgtcgcca gtccctcgaa ggagaaccca ggatgacccc
     7381 ctccgtaccc gggcacgtcg agcaagaggt cgagcaagaa accgtccggc tgaccctgaa
     7441 gtcgcgccgc gagatcgagg aggaaatcgc tcggcgggtc gccggggagc gcgagcgcct
     7501 gatcgggctc gccgcccctc gggccggggt cgcccacttc cgccggcctg cggagcggcc
     7561 gttcacggcc gccgagcgtg acgccgtcac gatcctgatt ggcggcttga cctggaagca
     7621 cgagcagctc atcagggccg tcttccagtc ggccggctac caggtggaga tcctgccgac
     7681 gcccgacgtg cagtcgttcc agctcgggaa ggagtacggc aacaacggcc agtgcaaccc
     7741 gacctacttc atggtcggcc acctgatcaa gttcctgcag ggcctcgaag cggccgggat
     7801 gacgcgccag gagatcatcg accgctacgt gttcttcacc gcggggtcgt gcggcccctg
     7861 ccgcttcggc atgtacgagt cggagtatcg gctcggcgtg cagaacgcgg ggttcgacgg
     7921 cttccgcatt ctgctcttcc agcagaacga cggcatcaag gccgcctcgg gcgaaccggg
     7981 gctgaagttc accgtcgatt tcgggctcgg cgccctgagc gcgctcaacc tcggcgacat
     8041 cgtcaacgac gtgacgtacc agatccggcc gtacgagatg gtaccgggcg aaaccgatcg
     8101 cgccctggcg gaggtgatgg aagacctgtc cgctcgcatg cgcgaccgcc ggatgttcga
     8161 ggcgatggac cgcctccccc gctggctggc ctcgcgcgtc gcgagccgga gggcgctgaa
     8221 gatcaccgtc agcgtcgtcg gcaagatcgc cgaccactgg tacggccgcc agacgcgcga
     8281 ggcactggcc gccgccagag agcagttgtc gcgcatcgag gtcgatcgcc tgcgcgtcaa
     8341 gcccctcgtg aaggtgaccg gggagttctt cgcgcagacc accgagggcg acgggaactt
     8401 cagaatgttc tccttcctcg agcgcgaagg ggcgcaggtg caggtcgagc cgatcggcaa
     8461 ctggatcatg tacctgctgt accaggcgcg cgagcgctac ctccagcaga ggcgcgtctc
     8521 caccgggaag ctgccggcgt ggcagctgcg ccgccgggcg gcc
//
LOCUS       DAOPQX010000144         8548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9254_length_8548_cov_4.541034, whole genome shotgun sequence.
ACCESSION   DAOPQX010000144 DAOPQX010000000
VERSION     DAOPQX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9254_length_8548_cov_4.541034"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /gene="acs"
                     /locus_tag="PKK95_09065"
     CDS             <1..1740
                     /gene="acs"
                     /locus_tag="PKK95_09065"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOC18405.1"
                     /translation="FNCVDRHLATLGDRTAIIWAQDEPGQYTHISYRELKHQVCRLAN
                     VLLSHGVRKGDRVCIYLTMMPQLVYAMLACARIGAVHSVVFGGFSSESLRDRIVDARC
                     KVVITANEGLRGGRHVPLKKTVDRAIEGMSMVETVLVAHRTETPVDMRPGRDWVLEEE
                     CHRHRSTATNEWMGAEDPLFILYTSGSTGKPKGLLHTTGGYLVYVGFTHKVIFDYHPG
                     DIFFCAADVGWVTGHSYVVYGPLLNGATTVLFESTPLYPDAGRYWRIVDDLGVNIFYT
                     APTALRALAQHGNEPVRRYRRSSLRVIGSVGEPINPEVWRWYHDVIGDGRCAVVDTWW
                     QTETGGILISPLPGATPAKPGAATQPFFGVKPVIVDPETGEPKNDGPAAGALCLASPW
                     PGQARTVWGDHQRFRETYFTKYPGYYFTGDGARRDEDGDYWITGRIDDVLNVSGHRLG
                     TAEVESALVAHEAVAEAAVVGYPHPLKGQGIYAYVLLNGDFPGGDPEQLAGALKQQVR
                     FMIGAFAAPDVIHIAAGLPKTRSGKIMRRILRKVAASEYTDLGDLTTLAEPDVVERLI
                     AEHKTLTARTSAA"
     gene            2118..4001
                     /locus_tag="PKK95_09070"
     CDS             2118..4001
                     /locus_tag="PKK95_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOC18406.1"
                     /translation="MTRRLVSVYSIALLLTVSPALASATEIIIRASDASTIRGNWHIA
                     YDDSAAGGQYLASNNNGWSKTDTPAASPADYFEASFSAPAGSYHVWLRLRATGNSKYN
                     ESVWVQYSDAVNSSGSAIYRIGTSSGLLVNLERCNNCGVSGWGWQDGAYWLSQSSTVR
                     FTTTGTHRIRVQTREDGVQIDQIVISSDNYLYESPGQLTNDRTIVSETSADSPSSSSV
                     GPYLGSPVRLPGRVEAENFDNGADGVSYHDVTSGNAGGAYRQTNVDIERCAEGGYNVG
                     WIQGGEWANYSVSVATAGSYTVRLRVASLSGGYLHVGFNGPSSVWSQVRVPATGGWQT
                     WRTVELPVKLGAGTQLLTLVYDSGSFNLNYVEVVAPTTTTTAPSTSGGTNVTVVTWNI
                     HYNANTSYAQRAMDLLMALSPRPQVVIIQEALRSQYNTYVNQLKARTGQTWSGHFANH
                     CPKGALSGGSCTSSEEEGVAVLTSLPVTSKSSRLLPYADAWHSARALVRVAVTVGGVV
                     VQVFGTHLQPDNPTARYNSMSVVKSYAAGYSKPQILGGDFNADADQICSSSGMSPAFI
                     DTWKLAGSGSGFTSRTPSPTMKLDYLFTDAGKRANPTSTRVATNTGTASDHYPVVTTF
                     SVR"
     gene            4243..6819
                     /locus_tag="PKK95_09075"
     CDS             4243..6819
                     /locus_tag="PKK95_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC18407.1"
                     /translation="MALDDALVRLPRPRPAVAWTAALAVVTVWGILRLVVFQSFNFPL
                     TYALPLLLCVWTRSGRMLWAMAGTFVANHTLRQFVFLSPAVADAPGAFWSYLATLANI
                     LVAALVVEAIIALRRRLEAAVHNAEEKRAEVFAQAARVREQAEELAAQNEELSRQAEE
                     LARIGEELNAQSEELAAQNEELRTQSEKIQRLNAELARRGDLLQTLLEATRTLRAEAA
                     VANHLCSAALDLIGPPSVVAAVREQRDGTIAVVGAAGVPQMVALPGPVPAGASFAGVV
                     MREDRTASLSDCSLRPDLALLEVTGERPFQSVLASPIHVAGRPVAVLEVYGHAPHEWT
                     AEQYRLVRWLALQGSQIIETLRLQGAIRRQAALIELSPDATIVQDGEGRVSFWSRGAE
                     ALYGWKREEALGRTTVELLHTQPDPAEIRRRVLEQGRVTCELTQATREGSVVTVESRW
                     LAQRGRDGEIEFLESNTDITERKRVEEALRRADEHKNRFLAILSHELRNPLAPIRYAL
                     QVLARNSKDSPSPADGDAAHAIEVIERQLGHLVRLVDDLLDVTRIASNKIKLQKRVVT
                     VARIVKQAVEAAMPVIRQARHELVVSPPPDDLCVDGDAERLAQVLINLLNNAARYTPD
                     GGVITLRSESENGEVVLSVSDTGIGLRAEDVPSLFQMFSQVGDSGRGGGLGIGLALVK
                     GVVELHGGHVEARSAGVGLGSTFLVRLPAAREAPAPRPEAPQITVASARRVLVVDDNP
                     DTALMMKALLELDGHEVFVAHDGTSALRMAHELLPQAVLLDIGLPSMDGYEVARGLRL
                     DPRTSQAFLVAVTGWGQEEDRRRAREHGFDAHLTKPADPEHIRRLVASAGGAAETKMD
                     AG"
     gene            complement(6836..8212)
                     /locus_tag="PKK95_09080"
     CDS             complement(6836..8212)
                     /locus_tag="PKK95_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18408.1"
                     /translation="MMLAREQMRFLRYVLATVVGLVVVLAVVPDRPLIVSRPVERLAS
                     VVVRGRDGAAPRLAVVAPTAGGAAGAVDEAIGVFGDDLAFEAAFELAALDEVDGLAAP
                     AREGDAGLRQWGERGVDGVLVVQPAVEGDRLRMAVRAFDVASGQVAFAREYLGPADAP
                     RTIGHVMANELHAAQANLHGVATTSLAFVSDRSGAKREPTGYMRRVKEIFVADYDGAN
                     QRRVTFDGDIVLTPSWSPDGAGLAYTSFQSGFQAVAFAKPGAGRDGRLRVGGKNWLPA
                     WAPDGRRIAFTSNRDGNEEIYVANRDGSGLTRLTRHWAIDTSPVWRPGGNRIAFTSGR
                     SGRPQIWIMDADGANQRQLTDERYCDRPTWSPPPFDEIAYVSRTKTGFDIKVIEVATG
                     VARQLTYGEGFNESPAWAPSGRHLAFTSTRVGGQQIWTMTRNGERLRQVTRVGNNSMP
                     AWGPLPGL"
     gene            8259..>8548
                     /locus_tag="PKK95_09085"
     CDS             8259..>8548
                     /locus_tag="PKK95_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18409.1"
                     /translation="MRQLVNGSIAGLAATVPMSAVMAALHRKLPSHEQYPLPPREVTV
                     EIARRAGAEAAVDSGTKREASTWLSHFGYGAAAGTAYGGLARRIPLGPMTGGV"
BASE COUNT         1317 a         2991 c         2949 g         1291 t
ORIGIN      
        1 ttcaactgcg tcgaccggca cctcgcgacg ctcggcgacc gcaccgcgat catctgggcc
       61 caggacgagc cgggccagta cacccacatc tcgtaccgcg aactgaagca ccaggtctgc
      121 cgcctggcca acgtcctcct ctcgcacggg gtgcgcaagg gcgaccgcgt ctgcatctac
      181 ctcacgatga tgccgcagct ggtgtacgcg atgctggcgt gcgcccgcat cggcgcggtc
      241 cactcggtgg tcttcggcgg cttcagctcc gagtcgctgc gcgaccgcat cgtcgacgcc
      301 cgctgcaagg tggtcatcac cgccaacgag gggcttcgag gcggacgcca cgtgccgctg
      361 aagaagacgg tggaccgggc catcgagggg atgtcgatgg tcgagacggt gctggtggcc
      421 caccgcaccg agacccccgt ggacatgcgg ccggggcgcg actgggtgct cgaggaggaa
      481 tgccaccgcc accgctcgac ggcgaccaac gagtggatgg gcgccgaaga cccgctcttc
      541 atcctctaca cctccggcag cacgggaaag ccgaaggggc tgctccacac caccggcggc
      601 tacctcgtgt acgtcggctt cacccacaag gtgatcttcg attatcaccc cggcgacatc
      661 ttcttctgcg ccgccgacgt gggctgggtc accggtcaca gctacgtcgt ctacggcccg
      721 ctgctgaacg gcgcgacgac cgtgctgttc gagtcgacgc cgctctatcc cgacgcgggg
      781 cgctactggc gaatcgtcga cgacctcggc gtcaacatct tctacacggc gcccaccgcg
      841 ttgcgcgcgc tcgcccagca cgggaacgag ccggtccggc gctaccggcg ctcgtcgctg
      901 cgggtgatcg gatcggtggg ggagccgatc aaccctgaag tctggaggtg gtaccacgac
      961 gtgatcggcg acgggcgctg cgcggtggtg gacacctggt ggcagaccga gaccggcggc
     1021 atcctgatct cgccgctccc cggtgcgact ccggcaaagc ccggggccgc cacgcagccc
     1081 ttcttcggcg tgaagccggt gatcgtggac cccgagaccg gcgagccgaa gaacgacggg
     1141 ccggccgcgg gcgccctctg cctggcgtcg ccgtggccgg gccaggcgcg cacggtctgg
     1201 ggcgaccacc agcgtttccg cgagacctac ttcaccaagt accccggcta ctacttcacg
     1261 ggcgacgggg cgcgccgcga cgaggacggc gactactgga tcacgggccg catcgacgac
     1321 gtcctcaacg tgtcggggca caggctgggg acggccgagg tggagagcgc gctggtggcc
     1381 cacgaggcgg ttgccgaagc cgcggtcgtc gggtacccgc acccgttgaa ggggcagggg
     1441 atctacgcct acgtgctgct gaacggcgac ttcccggggg gtgatcccga gcagttggcg
     1501 ggcgcgctga agcagcaggt ccggttcatg atcggggcgt tcgccgcgcc cgacgtcatc
     1561 cacatcgccg cggggctgcc caagacgcgc agcggcaaga ttatgcgccg catcctgcgc
     1621 aaggtggccg cctccgagta caccgacctc ggcgacctca cgacgctggc cgagcccgac
     1681 gtcgtcgagc ggctgatcgc cgagcacaag acgctcacgg cgaggacctc ggcggcgtag
     1741 cgaaacgctt catttccagc ccggcagggg gcggcgccgc ccgccagtgg cgcgccgccg
     1801 tcttttcata caactcagcc gtattattgt tcttggtcca atacggcctg ttttacacat
     1861 gaatcaagct catccggatt cgaccgtgta acttcttcca tatggatgag ttgcctcaag
     1921 ggacaacgga tgagacggcc acgtccgccg ctctcctaag gccctagccg cgagcgagtt
     1981 acacggtctg gccgcctcct tgccctcggg ggtccccagt cgctgccccc catagttgcg
     2041 accttccttt gatgattccc gggcgtgcga cggcaacagt gtgccgcgtg cgccgccccg
     2101 cgtttcaagg aggcccgatg acgcgacgac tcgtgagtgt gtattcgatc gccctgctcc
     2161 tgacggtcag ccccgcgctg gcatccgcaa ccgaaatcat catccgggca tccgacgcca
     2221 gcaccatccg cggcaactgg cacatcgcct acgacgactc ggcggccggc ggtcagtacc
     2281 tggccagcaa caacaacggc tggtcgaaga ccgacacgcc agccgcgtcg cccgccgact
     2341 acttcgaggc gtcgttcagc gcccccgcgg gctcgtatca cgtctggctg cgcctcaggg
     2401 cgaccggcaa cagcaagtac aacgagtcgg tctgggtgca gtactccgac gcggtgaaca
     2461 gctcggggag cgcgatctac cgcatcggca cgtcgagcgg cctgctggtg aatctcgagc
     2521 ggtgcaacaa ctgcggcgtc tcggggtggg gctggcagga cggggcctac tggctctcgc
     2581 agtcctcgac ggtgcgcttc acgacgacgg ggacgcaccg gatccgggtg cagacccgcg
     2641 aagacggcgt gcagatcgat cagatcgtca tcagctcgga caactacctg tacgagtcgc
     2701 ccggccagct gaccaacgac cggacgatcg tctccgagac cagcgccgac tcgccttcgt
     2761 cgtcctcggt aggcccgtac ctcgggtcgc cggtgcgcct gcccggccgc gtcgaggccg
     2821 agaacttcga caacggggcc gacggcgtgt cctaccacga cgtgacgagt ggcaacgccg
     2881 gcggcgccta ccggcagacg aacgtcgaca tcgagcggtg tgccgagggc ggctacaacg
     2941 tcgggtggat ccagggcggc gagtgggcca actactcggt gagcgtggcc acggcggggt
     3001 cgtacaccgt gcgcttgcgt gtggcgtcgc tgagcggcgg gtatttgcac gtgggcttca
     3061 acgggccgag cagcgtgtgg tcgcaggtgc gggtgccggc gaccggcggc tggcagacgt
     3121 ggcggacggt ggagctgccg gtgaagctgg gtgccggaac gcagctgctg acgctcgtct
     3181 acgacagtgg cagcttcaac ctgaactacg tggaggtcgt cgcgccgacc accaccacca
     3241 cggcgccctc gacgagcggc ggcaccaacg tcaccgtcgt cacctggaac atccactaca
     3301 acgccaacac ctcgtacgcg cagcgggcca tggacctgct gatggcgctc agcccgcggc
     3361 cccaggtggt gatcatccag gaggcgctgc ggtcgcagta caacacctac gtcaaccagc
     3421 tcaaggcgcg caccgggcag acctggtcgg gccacttcgc caaccactgc ccgaaggggg
     3481 ccttgagcgg cggcagctgc acgtcgagcg aagaggaggg ggtcgcggtc ctgacctcgc
     3541 tgccggtcac cagcaagagc tcgcgcctgc tgccgtacgc cgacgcgtgg cactccgccc
     3601 gcgcgctcgt gcgggtggcg gtgaccgtcg gcggcgtcgt ggtacaggtg ttcggcaccc
     3661 acctccagcc cgacaacccg acggcccgct acaactcgat gtcggtcgtc aagtcctacg
     3721 cggcgggcta ctccaagccg cagatcctgg gcggcgactt caacgccgac gccgaccaga
     3781 tctgctcgtc ctccgggatg tcgccggcct tcatcgacac ctggaagctg gcgggctcgg
     3841 gcagcgggtt cacgtcgcgg accccgtcgc ccaccatgaa gctcgactac ctgttcaccg
     3901 acgcggggaa gcgggcgaac ccgacctcga cccgtgtcgc gaccaacacc ggcacggcgt
     3961 ccgaccatta cccggtggtg acgaccttct ccgtgcgctg atttcccctc cctctcccgt
     4021 cccctcgagg acgcactccg atgcgagtgc gtcctcctct ttttgtcttg tgggggcgtc
     4081 tcgcgttcaa atccgcgccg aacgcctgta ttctgatttg accggaacat ctggcgcggc
     4141 gggccccggg tgctgctgca ggcctgcctc gacccgcacg cccatcgcgc gcgggtcttc
     4201 aagcggcgga ttccgccgtg ctcgaggccc gacggaggcg gaatggcact tgacgatgcg
     4261 ctggttcgcc tgccgaggcc gcggccggcc gtcgcatgga cggcggcgct ggccgtcgtc
     4321 accgtgtggg ggatcctccg ccttgtcgtc ttccagtcct tcaactttcc gctgacctac
     4381 gccctgccgc tgctgctatg cgtctggacg cggagcgggc ggatgctgtg ggcgatggcg
     4441 gggaccttcg tcgccaacca cacgctcagg cagttcgtat tcctgtcccc ggctgtcgcc
     4501 gacgcgcctg gtgcgttctg gagctacctc gccaccctgg cgaacatcct cgttgccgcg
     4561 ctggtggtcg aggcgatcat cgcgctgcgg cggcgcctcg aggctgcggt ccacaacgcc
     4621 gaggagaagc gtgccgaggt gttcgcgcag gccgccaggg ttcgcgagca ggcggaggag
     4681 ctggccgcac aaaacgagga gctttcgcgc caggccgagg aactggcccg gattggggaa
     4741 gagctgaacg cccagtccga ggagctggcg gcgcagaacg aggagttgag gacgcagtcc
     4801 gagaagatcc agcggctcaa cgccgagctc gcccgccggg gggatctcct gcagacgctg
     4861 ctcgaggcga cccgcacgct gcgtgccgag gccgccgtgg ccaaccacct gtgctcggcg
     4921 gcgctcgacc tgatcggccc tccctcggtg gtggcggccg ttcgcgagca gcgcgacggg
     4981 acgatcgcgg tcgtgggggc ggcgggcgtt ccccagatgg tcgcgcttcc cgggccggtg
     5041 cccgccgggg cttcgttcgc cggcgtggtc atgcgcgagg accggaccgc gtccctttcg
     5101 gattgctcgc ttcgccccga ccttgccctg ctggaagtca ccggggagcg ccccttccag
     5161 tcggtcctcg cgtcgccgat acacgtggcg ggccgtccag tggcggtgct ggaggtctac
     5221 gggcacgccc ctcacgagtg gacggcggag cagtaccgcc tcgttcgctg gctcgcgctc
     5281 cagggatcgc agatcatcga gacgctgcgc ctccagggcg ccatccggcg gcaggcggcg
     5341 ctcatcgagc tgagccccga tgcaaccatc gtccaggacg gcgaaggaag agtctccttc
     5401 tggagccggg gagccgaagc gctctatggc tggaagcgcg aggaggcgct cgggcggacc
     5461 accgtcgagt tgctgcacac gcagcccgac cccgccgaga tccggcggcg ggtgctggag
     5521 cagggtcgcg tcacctgcga gctgacgcaa gcgacgcggg agggcagcgt cgtcacagtc
     5581 gagagccggt ggctggcgca gcggggccgg gacggtgaga tcgagttcct cgagtcgaat
     5641 accgacatca ccgagcgcaa gcgggtcgag gaagcccttc gccgggcgga cgagcacaag
     5701 aaccgctttc tggccattct ctcgcacgag ctgcggaacc cgctcgcccc gatccgctac
     5761 gccctccagg tgctggcgcg caactcgaag gactcgcctt cacccgccga tggcgacgcc
     5821 gcccacgcga tcgaggtgat cgagcggcag ctgggccacc tcgtgaggct cgtggacgac
     5881 ctgctcgacg tcacccgcat cgcgagcaac aagatcaagc tgcagaagcg ggtggtcacg
     5941 gtggcccgca tcgtcaagca ggcggtggag gcggcgatgc cggtcatccg gcaggcgcgg
     6001 cacgagctgg tcgtgtcgcc gccgcccgac gacctctgcg tggacggcga cgccgagcgg
     6061 ctcgcccagg tgttgatcaa cctgctgaac aacgccgccc gctacacccc cgatggcggc
     6121 gtgatcaccc tccggtccga gtccgagaac ggggaggtgg tgctgtcggt cagcgacacc
     6181 gggatcgggc tgcgggccga ggatgtgccg tcgctctttc agatgttcag ccaggtcggc
     6241 gactccggcc gcggcggcgg tctcggcatc gggctggcgt tggtaaaggg cgtcgtcgag
     6301 ctgcacggcg ggcacgtcga ggcgcgaagc gccggggttg gcctaggtag caccttcctc
     6361 gtccgcctgc cggctgccag ggaggccccc gcgccgaggc ccgaggctcc gcagatcacg
     6421 gtcgcatccg cgcggcgcgt tctcgtggtg gacgacaacc ccgacacggc cctgatgatg
     6481 aaggccttgc tcgagctgga cggccacgag gtgttcgtcg cccacgacgg aacgtcggcc
     6541 ctgcgcatgg cgcacgagct gcttccccag gccgtgctgc tcgacatcgg cctgccctcg
     6601 atggacggtt acgaggtcgc gcgcggcctg cgcctcgatc cgaggaccag ccaagccttc
     6661 ctggttgccg tgaccgggtg gggacaggaa gaggaccgca ggcgggcccg ggagcacggc
     6721 ttcgacgctc acctcaccaa gccggccgac cccgagcaca tcaggcgcct cgtggcgtcg
     6781 gcgggcgggg cggcggagac gaagatggac gcgggataga cgaaggttca gggggtcaga
     6841 ggccgggcag cggcccccac gcgggcatcg agttgttacc gacccgcgtc acttgccgga
     6901 gccgttcgcc gtttcgggtc atggtccaga tctgctgccc gcccacccgc gtcgaggtga
     6961 aggcgaggtg gcggcccgag ggcgcccacg ccgggctctc gttgaagccc tccccgtaag
     7021 tcagttgccg cgccacgccg gtggccacct cgatcacctt gatgtcgaag ccggtcttgg
     7081 ttcgcgagac gtaggcgatc tcgtcgaacg ggggcggcga ccaggtgggc cggtcgcagt
     7141 agcgctcgtc ggtgagctgc cgctggttgg cgccgtcggc gtccatgatc cagatctgcg
     7201 gccgccccga gcgccccgag gtgaaggcga tccggttccc gcctggtcgc cagacgggag
     7261 aggtgtcgat ggcccagtgg cgcgtgaggc gcgtcaggcc ggagccgtct cggttggcga
     7321 cgtagatctc ctcgttcccg tcgcgattcg aggtgaaggc gatgcggcgc ccgtcaggcg
     7381 cccaggccgg cagccagttc ttcccgccga ctctcagcct gccgtcgcgg cccgcgccgg
     7441 gcttcgcgaa ggccaccgcc tggaacccgc tctggaacga ggtgtaggcg aggcccgccc
     7501 cgtcgggcga ccagctcggt gtcaacacga tgtcgccgtc gaaggtcacg cggcgctggt
     7561 tggcgccgtc gtagtcggcg acgaagatct ccttcacccg ccgcatgtag ccggtcggct
     7621 cgcgcttggc gcccgatcgg tccgacacga acgcgagact cgtcgtagcc acgccgtgca
     7681 ggttcgcctg ggccgcgtgc agctcgttgg ccatcacgtg gccgatcgtc cgcggggcgt
     7741 cggccggccc caagtactcc cttgcaaagg ccacctggcc gctggccacg tcgaaggcgc
     7801 ggacggccat tcgcagccga tcgccttcga ccgccggctg aaccacgagg acgccgtcca
     7861 ctccgcgctc gccccactgg cgcagcccgg cgtctccttc gcgagcgggc gccgccaggc
     7921 catccacctc gtccagagcg gccagttcga aggctgcctc gaaggcgagg tcgtccccga
     7981 acacgccgat ggcctcgtcg acggccccgg cagccccgcc ggcagtcggc gccaccacgg
     8041 cgagccgcgg cgcggcacca tcccgtcccc gcacgaccac cgacgcgagc cgctcgaccg
     8101 gccgcgacac gatcagcgga cggtcgggca cgacggcgag gacgacgacg aggcccacaa
     8161 cggtggcgag cacgtagcgg aggaagcgca tctgttctct ggccagcatc attatcgccg
     8221 ctctccagcc tggcactctc ttggcagcag aaaccgccat gcgccagctc gtgaacggat
     8281 cgattgcagg cctcgcggcc accgtcccga tgagcgccgt catggcggcg ctccaccgga
     8341 agcttccttc ccatgaacag tatccgctgc cgccgcggga ggtcacggtt gagatcgccc
     8401 gccgtgccgg cgcggaagcg gcggtggact cgggaacaaa gcgcgaagcc tccacctggc
     8461 tttcccactt cgggtacggc gcggcggccg ggacggccta cggcggactc gcgcggcgca
     8521 tccccctggg gccgatgact ggaggggt
//
LOCUS       DAOPQX010000145         8517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9292_length_8517_cov_4.070905, whole genome shotgun sequence.
ACCESSION   DAOPQX010000145 DAOPQX010000000
VERSION     DAOPQX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8517
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9292_length_8517_cov_4.070905"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKK95_09090"
     CDS             <1..826
                     /locus_tag="PKK95_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC18410.1"
                     /translation="PLAPSLLAACPRLRMIAHCGGEVKARFARPLFDRLTITNAADPM
                     ARQVAELAAAFLLYEARNVDRYRAQIRCPGTGIFEARHQRGVEGETLAGATVGLVGFG
                     RIGRALVELLVPFGARFVVYDPYARRRVPRGARVRFAPLDRVLGASRLLVLACALTED
                     TRNLLDRERLARLPDGASIVNVARGGLVDLEALTAEVVAGRLRCALDVTDPLEPLPDD
                     HPLRVAPGAILTPHVGAGADRVRRAMANVVLDELERYFSGRRVRHRVSRAMLDRMT"
     gene            836..2536
                     /locus_tag="PKK95_09095"
     CDS             836..2536
                     /locus_tag="PKK95_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOC18411.1"
                     /translation="MLKSVTAVDYAVIAAYLLAMLAIGASFMRFNKGASDYFRGGSRI
                     PWFVAGLSSFMSGFSAWTFTGAAGVAYQQGLVVVLLYVGNGLSFLLGYWLFASRWRRA
                     RISTTMEYLTERFDERTRQTFSWTAVIFHLFTGASVLFGLGVFVSSTCGVPIGWTILA
                     CGAIILVYCVVGGLWAVVVTDFLQAVILLPFTLVLCAVSLSRIGGVKAFLEGLPAPMT
                     SLALPNEFGWGYVAAWTVMVSFGYNTSVMAQRYFAVDDERSARKVSLLCCGLFLLGSL
                     LWFVPPLAVRVLYPDIAALLPGRANPHEASYAVATLALLPNGLIGVMLAAMFSSTMSN
                     LSGTFHLHAAIIVRDIYLWIFPRRDGERHLLAVGWVATFFVGASIIALAFVMAESGRS
                     VFSVMLTVNTIMSLAYGPPALLGLLISRTPRWSGLASFLVGLALGCVGTFGFGWGLIA
                     NVVGVLPASIAVFLLSIPFERRDAAGAAGRRRLAERLATPVDVARELGASEDRTTEVF
                     RFLSRATAGVGLVSLLLMAWAAPGERGTVVLYSGITLLVAVLLALVKGAPAPARAERT
                     "
     gene            2547..4325
                     /locus_tag="PKK95_09100"
     CDS             2547..4325
                     /locus_tag="PKK95_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOC18412.1"
                     /translation="MTTRLARLGCLVSLILAVTPVASPAGVKRVWAVNDTEKIARENL
                     SSPLAAGNSAWDGRSVKLFGARNEVLAVQVIVEADERGIASLSLRLPELKQRGGTARF
                     AYVPPGADPTDSVGRPIQLFALNYMHVPGPATAHWIYRAGTESAARGMGWQPVQIVPE
                     NARSGRGGFPLGVPPSSNQAFWIEIYTGKERPAGIYEGAIEIVADGRTIRLPIELELF
                     GFALPDENSLNLMVYFEGSQPERYMGRNFDAEFHRFAHRHRVELVHAYDEAALRQAFA
                     RFTGAAFTPAERYEGPGETVGHRFAPATFYGPGRAFESRESARSRSDAWMTFLEKTLP
                     GAITFLYLPDEPGPAQYPRLRELADYVHSNPGPGRRLKTFATKEYVPELDGAVDIWCA
                     PPQTYDIARARSERAKGREYWTYNGSRPFLGVPSIEAPAADPRSMAWAAFKENIPTFF
                     YWHGVHWFHNSQKQGDRRQNVWANPVTFDNRGQPGKEDTGFAYGDGVLIYPGLEVVHP
                     EEDRGIAGPVSTVQLANYRRGLQDHLYLTLARKAGLGDLVDEALRAIVPRVFSEADAK
                     SKVGWAEAGDAYEAMRLKIGRALAAK"
     assembly_gap    4397..4496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4504..6714)
                     /locus_tag="PKK95_09105"
     CDS             complement(4504..6714)
                     /locus_tag="PKK95_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC18413.1"
                     /translation="MLLVAAAVAALAVTAWLVSRGNNPESGGAASGPRAGAPNILLVT
                     LDTVRADHLGCYGNQAARTPVIDALAARGARFATGVAHSPITGPSHASILTGLTPLGH
                     GVRNNSDYVLPVSVPTLPDALAKAGWRTAAFVSGFPLERRFGYDRGFETYDAAFPRGN
                     DPRRAPYVERPADMTTRAALKWLDEGASSSPAPWFLWVHYYDPHSPYEPPAEYADAFA
                     GRPYDGEIAFVDAQLGRLLQRIDERGLTGNTLIVVTADHGESLGEHGEATHGMFVYDS
                     TLLVPWIVAGPGIPAGRVPPMVARLIDVAPTLLDYAGQRPAGAIEGRSLRPAIDGQPM
                     SDEPAYAESLFASIHLRWAPLYAWRTSRWKLVDAPRPELYDLQADPREQHDRAAAEAA
                     RVEALRRPLRAALRTPTPDATATVDAESAERLRSLGYLGGGPFSPETASSKRDPKDAL
                     PLVARLERGMELARIDPATAVSELTAVLREAPEAVLARRHRAVALGVLGRANEAVADM
                     HAIEKAGPMTAEDLVVLAESLRLAGRADEAIAALDRASALQPRLVPAWLARADVLVNA
                     QRTEEASRAFEQVLAIAPEHSEALRGLGDLALVRGDLAAAGGFYGRVLAAAPDDGPAS
                     VKLGVVRMRTGQREEALALFRKAIERNPKDGEALLYAAGALVATGRPQEALAFFDRAI
                     AAGQRTAIAYAGLGMTRLQLGDERGAAAALGESLALDPNQPQVADTLKKLRTRR"
     gene            6943..>8517
                     /locus_tag="PKK95_09110"
     CDS             6943..>8517
                     /locus_tag="PKK95_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOC18414.1"
                     /translation="MRVQVGAGRVWRRLAWVAVTTLVLALVAPATGAAQSVLGSIRGT
                     VTDTGGGVIPKAPVLITDETTGLPRTLDTDSRGVFEVVNLQPGTYRVEVIVPGFRKFE
                     RPGIIVRTGQAALVNVELEIGPIGESVTVTGSAPANVITLDSPSVTKGLDEQQLRDLP
                     RASRDIQSFLYLNPNVAGTAEGDMQFLGGRTYGVSYVQDGQASTNAIFGTVGNSAPGL
                     DAIQEMQVLSNSYSAEYGGLAGVVVTTKRGGQKFHGSAFWDFNSNELNQLTYGQKLTG
                     VSRDDPNADTSVHRYGGALGGPLFKRTFFYANYEGLRDKTIQGGGTITVPGAALRAGD
                     FSASTITVRDPETGEPFPDNQIPAERISPIAQNIMNMFWPMPNRSVVASGMGTFQQYV
                     PVKRKRERFDLRLDHEAGTGNQFFARGSYQYRNPTRMQWEAGAALTQLPLVKSAMNTY
                     ALVGGWTSILSSRLVNEVRVGYNFDGSERKSTWQAGDIAAKMGIELPPSVSPDTPGFP
                     SINFVAGSNRPGNIADG"
BASE COUNT         1163 a         2915 c         3064 g         1275 t
ORIGIN      
        1 gccgctcgcc ccgtcgctgt tggcggcctg tccgcggttg cgaatgatcg cgcactgcgg
       61 cggggaggtg aaggcgcggt tcgcccggcc gctcttcgac aggctgacga tcaccaacgc
      121 cgccgatccc atggcccgcc aggtggccga gctggcagct gcctttctgc tctacgaggc
      181 acggaacgtc gatcggtacc gggcgcagat ccggtgcccg gggaccggca tcttcgaggc
      241 gcgccaccag cggggggtgg agggcgagac gctggccggc gccacggtgg ggctcgtcgg
      301 cttcggccgc atcggccgcg cgctggtgga actgctcgtg ccgtttggcg cccggttcgt
      361 ggtctacgac ccctacgcgc gccgccgcgt tccccgcggg gcgcgcgtaa gattcgcacc
      421 gctcgaccgc gtcctcgggg catcgcgcct gctcgtgctc gcgtgcgcgc tcaccgagga
      481 cacgcggaac ctgctcgacc gcgagcgtct ggcgcgcctg cccgacggcg cctcgatcgt
      541 caacgtggcg cgcggaggcc tggtggatct cgaggcgctg accgccgagg tcgtggccgg
      601 ccggctgcgc tgcgcgctcg acgtgacgga cccgctggag ccgctgcccg acgatcaccc
      661 ccttcgggtc gcgccggggg ccatcctgac gccccacgtc ggggcggggg ccgaccgcgt
      721 gcgccgggcg atggcgaacg tcgtgttgga cgagctcgaa cggtacttct ccggccggcg
      781 cgtgcggcat cgcgtgagcc gggccatgct ggaccggatg acctgagcca ttcgcatgct
      841 gaagagcgta accgccgtcg actatgcggt catcgccgcc tacctgctgg cgatgctcgc
      901 catcggcgcc tccttcatgc gcttcaacaa gggggcgtcc gactacttcc ggggcggcag
      961 ccggattccg tggttcgtgg ccggcctcag ctccttcatg agcggcttca gcgcctggac
     1021 gttcacgggc gcggccggcg tggcgtacca gcaggggctg gtcgtcgtcc tgctgtacgt
     1081 cgggaacggc ctcagcttcc tgctcgggta ctggctcttc gcgtcgcgct ggcggcgggc
     1141 gcgcatctcg accacgatgg agtacctgac ggagcggttc gacgagcgga cgcgccagac
     1201 cttctcgtgg accgccgtga tcttccacct gttcacgggc gccagcgtgc tcttcggcct
     1261 cggcgttttc gtcagctcga cgtgcggggt gccgatcggg tggaccatcc tggcctgcgg
     1321 cgccatcatc ctcgtctact gcgtggtggg cggcttgtgg gcggtcgtgg tcaccgactt
     1381 cctgcaggcg gtgatcctcc tgccgttcac gctggttctc tgcgccgtct cgctctcgcg
     1441 aatcggcggc gtgaaagcat tccttgaggg gctgccggcg ccgatgacgt cgctcgcgct
     1501 gccgaacgag ttcgggtggg gatacgtggc ggcgtggacg gtaatggtga gcttcggcta
     1561 caacacgtcg gtcatggcgc agcgctactt cgccgtggac gacgagcggt cggcacggaa
     1621 ggtgtcgctg ctgtgctgcg gcctcttcct gctggggtcg ctgctctggt tcgtgccgcc
     1681 gctggccgtc agggtcctgt accccgacat cgcggcgctc ctgccggggc gcgcgaaccc
     1741 gcacgaggcg tcgtacgcgg tcgccacgct ggccctcctg ccgaacggcc tgatcggggt
     1801 gatgctggcg gcgatgttca gctcgacgat gtcgaacctg tcggggacgt tccacctcca
     1861 cgcggcaatc atcgtgcgcg acatctacct gtggatcttc ccccggcgcg acggcgagcg
     1921 gcacctgctg gcggtcggct gggtggccac cttcttcgtg ggcgcgagca tcatcgcgct
     1981 ggccttcgtg atggccgaga gcgggcgcag cgtgttcagc gtgatgctga cggtgaacac
     2041 gatcatgtcg ctcgcatacg gcccgcccgc gctgctcggc ctgctcatct cgcgcacgcc
     2101 gcgctggtcg gggctcgcgt cgttcctggt cggcctggcg ctcgggtgcg tggggacctt
     2161 cgggttcggc tgggggctga tcgcgaacgt ggttggcgtg ctgcccgcct cgatcgcggt
     2221 gttcctcctg tcgattccct tcgaacgtcg cgacgcggcg ggggcggcgg ggcggcgccg
     2281 cctggccgag cggctggcca cgccggtgga cgtcgcgcgg gagctgggcg cgagcgagga
     2341 ccgtaccacc gaggtcttcc gcttcctgtc gcgggcgacg gcgggcgtgg ggctcgtcag
     2401 tctgctcctg atggcctggg ccgcaccggg cgagcgcggc accgtcgttc tctattcggg
     2461 catcacgctg ctcgtcgccg tgctgctggc gctcgtcaag ggcgcccccg cgcctgcccg
     2521 cgcggagcgc acgtagggag agatccatga ccacccgact tgcccgcctg ggatgcttgg
     2581 tttccctgat cctggcggtg acgcccgttg cgtcgccggc cggcgtgaag cgcgtctggg
     2641 ccgtcaacga caccgagaag attgcgcgcg aaaatctctc gagcccgctg gcggcgggga
     2701 actcggcctg ggacgggaga agcgtgaagc tgttcggcgc acgcaacgag gtgctggccg
     2761 tgcaggtgat cgtcgaggcc gacgagcggg gcatcgcgtc gctctcgctg cggctccccg
     2821 aactgaagca gcgtggagga acagcacgct tcgcctacgt tccgccgggc gccgatccga
     2881 ccgactcggt tggccggccg atccagctgt tcgcgctgaa ctacatgcac gttcccggtc
     2941 ccgcgaccgc gcattggatc tacagggccg gcaccgagtc ggccgcgcgc ggcatggggt
     3001 ggcagccggt gcagatcgtc cccgagaacg cccgcagcgg gcgcggcggc ttcccgctcg
     3061 gcgtgccgcc gtcgagcaac caggcgttct ggatcgagat ctacaccggg aaggagcgcc
     3121 cggccggcat ctacgagggc gccatcgaga tcgtggccga cggccggacg atccgcctgc
     3181 cgatcgagct ggagctgttc gggttcgccc tgcccgacga gaacagcctg aacctgatgg
     3241 tgtacttcga gggcagccaa cccgaacggt acatgggccg gaacttcgac gccgagttcc
     3301 accgtttcgc gcatcgccac cgtgtcgagc tggtgcacgc gtacgacgag gcggcgctcc
     3361 ggcaggcgtt cgcgcgcttc accggcgccg cgttcacgcc ggcggaacgg tacgaaggcc
     3421 ctggcgagac ggtgggccac cgcttcgcgc ctgcgacctt ctacggcccc ggccgcgcct
     3481 tcgaatcgcg cgagtcggcc cggtcgcggt cggacgcctg gatgacgttc ctcgagaaga
     3541 cgctgccggg cgccatcacg ttcctgtacc tgccggacga gccggggccc gcccagtatc
     3601 cccggcttcg cgagctggcc gactacgtgc actcgaaccc gggccccggg cggcgcctga
     3661 agacgttcgc gaccaaggag tacgtgcccg agctcgacgg ggcggtggac atctggtgcg
     3721 cccccccgca gacgtacgac atcgcgcgtg ccaggtcgga gcgggcgaag gggcgcgagt
     3781 actggaccta caacggcagc cggccgttcc tcggggtccc gtcgatcgag gcgccggcag
     3841 ccgacccgcg gtcgatggcg tgggcggcct tcaaggagaa catcccgacg ttcttctact
     3901 ggcacggcgt gcactggttc cacaacagcc agaagcaggg cgaccggagg cagaacgtgt
     3961 gggcgaaccc ggtcaccttc gacaaccgcg gacagcccgg caaggaggac accggatttg
     4021 cctatgggga cggcgtgctg atctacccgg ggctcgaggt cgtgcacccc gaagaggacc
     4081 gcggcatcgc gggccccgtg tccaccgtgc agctggccaa ctaccggcgc ggcctgcagg
     4141 atcacctgta cctcaccctg gcgaggaagg cggggctcgg cgacctggtg gacgaggcgc
     4201 tgcgggcgat cgtgccgcgc gtgttctccg aggcggacgc gaagtcgaag gtgggctggg
     4261 cggaggcggg cgacgcctac gaggcgatgc ggctgaagat cggccgcgcc ctcgccgcga
     4321 agtagacgcg ggtccacgtt aaccacagag gcacagagaa cacagagatt agatgcttgt
     4381 ccacgttcaa cgcagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggtg
     4501 ccgtcagcgg cgcgtgcgga gcttcttcag cgtgtcggcg acctgtggct ggttcggatc
     4561 gagggcgagc gactcgccga gcgcggcggc cgcgccccgc tcgtcgccca gctgcagcct
     4621 cgtcatgccg aggcccgcgt aggcgatcgc ggttcgctgg ccggccgcga tggcgcggtc
     4681 gaagaaggcg agggcctcct gcggccggcc ggttgcgacc agggcacccg cggcgtagag
     4741 cagcgcctcc ccgtccttcg ggttccgctc gatcgccttc ctgaacagcg cgagcgcctc
     4801 ctcgcgctgc ccggtccgca tccggacgac ccccagcttc accgacgccg ggccgtcgtc
     4861 cggcgcggcg gccagcacgc ggccgtagaa gccgcccgcc gccgccaggt cgccccggac
     4921 gagcgccagg tcgccgaggc cgcgcagggc ctccgagtgc tcgggcgcaa tcgccagcac
     4981 ctgctcgaac gcgcgggacg cttcctcggt ccgctgcgcg ttcaccagga cgtcggcgcg
     5041 cgccagccag gcgggaacca ggcgcggctg cagggcggag gcccggtcca gcgccgcgat
     5101 cgcctcgtct gcgcgccctg ccaggcggag gctctcggcg agaacgacga ggtcttcggc
     5161 ggtcatggga cccgccttct cgatcgcgtg catgtcggcc accgcctcgt tcgcgcgccc
     5221 cagcacgccc agcgccacgg cgcggtgccg ccgggcgagc acggcctcgg gggcctcgcg
     5281 cagcaccgcc gtcagctccg acaccgccgt ggcgggatcg atccgcgcca gctccatgcc
     5341 ccgctcgagg cgcgccacca gcggcagcgc gtccttggga tcgcgcttcg acgaggcggt
     5401 ctcgggcgag aaggggccgc cgccgaggta cccgagcgaa cgcagccgct ccgccgattc
     5461 cgcgtccacc gtggcggtgg cgtcgggcgt cggggtgcgg agcgccgcgc gcaggggcct
     5521 gcgcagcgcc tccacgcgcg ccgcctcggc ggcggcgcgg tcgtgctgct cccgcggatc
     5581 ggcctggagg tcgtacagct cgggccgggg agcgtcgacg agcttccagc gcgacgtccg
     5641 ccaggcgtac agcggcgccc accgcaggtg gatgctcgcg aagagcgact cggcgtacgc
     5701 cggctcgtcg ctcatcggct ggccgtcgat cgccgggcgc agtgaacggc cctcgatcgc
     5761 gccggccggc cgctggcccg cgtagtcgag gagggtcggc gccacgtcga tcaggcgggc
     5821 caccatcggc ggcacccgtc cggccggaat gcccggccct gccacgatcc agggcaccag
     5881 cagcgtcgag tcgtagacga acatgccgtg cgtggcttcg ccgtgctcgc cgaggctctc
     5941 gccgtggtcg gccgtcacca cgatgagcgt gttgccggtg aggccgcgct cgtcgatgcg
     6001 ctgcagcagg cggcccagct gcgcgtccac gaacgcgatc tccccgtcgt acgggcggcc
     6061 ggcgaaggcg tccgcgtact cggcgggcgg ctcgtacggg gagtgcgggt cgtagtagtg
     6121 cacccagagg aaccacggcg ccggtgaaga cgaggcaccc tcgtccagcc acttcagggc
     6181 ggcgcgcgtg gtcatgtccg cggggcgttc gacgtacggc gcgcgccttg gatcgttccc
     6241 gcgcgggaac gccgcgtcgt aggtctcgaa gccccggtcg tacccgaaac gccgctccag
     6301 cgggaagccc gaaacgaatg ccgcggtgcg ccagcccgct ttcgcgagcg cgtcgggcag
     6361 cgtgggcacg ctgaccggca gcacgtagtc ggagttgttc cggacgccgt gcccgagcgg
     6421 ggtcagcccc gtcaggatcg aggcgtgcga ggggccggtg atgggggagt gcgccacccc
     6481 ggtcgcgaac cgggcgccgc gcgcggcgag cgcgtcgatg accggcgtgc gcgccgcctg
     6541 gttgccgtag cagcccaggt gatcggcgcg aaccgtgtcc agcgtgacga ggaggatgtt
     6601 cggggcgccc gcccgcggcc ctgacgccgc cccgcccgac tccgggttgt tccctcgcga
     6661 caccagccac gccgtcaccg cgagcgcggc cacggcggca gccaccagca acacggggag
     6721 ccatccccgg cgccgtggag gcgcgccggc ggcgggacgg actggggttg ccatgcgacg
     6781 gggcgggcgt ctttcgcggg aaggcacgcc gaatcgtacc ggcggccttg cctggcggtc
     6841 aacaatctct tgacttacga ttcgtaaagt aatagaaagc gtgcatcgta tcttttgctt
     6901 gcgtttgcca ccttttggag gggttccggc gaggaggatg ccatgcgagt gcaagtcggc
     6961 gctgggcggg tgtggcggcg gctggcgtgg gtggctgtga caacccttgt cctggcgctc
     7021 gtggcgccgg cgacgggcgc ggcgcagtcc gtgctcgggt cgattcgcgg gacggtgacc
     7081 gatacgggcg gaggcgtcat tcccaaggcg cccgtgttga tcaccgatga aacgaccggg
     7141 ctgccccgga cgctcgacac cgacagccgg ggagtgttcg aggtggtcaa cctccagccc
     7201 ggcacctacc gggtcgaggt gatcgtcccg gggttcagga agttcgaacg ccccggcatc
     7261 atcgtccgaa ccggccaggc ggcgctggtg aacgtggagc tggagatcgg gccgattggc
     7321 gagagcgtga cggtgacggg ctcggcgccc gccaacgtca tcacgctcga cagcccgtcg
     7381 gtgaccaagg ggctcgacga gcagcagctg cgcgacctgc cgcgcgcctc ccgcgacatc
     7441 cagtcgttcc tctacctgaa cccgaacgtg gccggcaccg ctgaaggcga catgcagttc
     7501 ctgggcgggc gcacctacgg cgtgtcgtac gtgcaggacg gccaggcgtc caccaacgcc
     7561 atcttcggca cggtaggcaa ctcggcgccc ggcctcgacg cgatccagga gatgcaggtg
     7621 ctctcgaact cgtacagcgc cgagtacggg ggcctggccg gggtggtcgt caccaccaag
     7681 cgcgggggcc agaagttcca cgggtcggcg ttctgggact tcaactcgaa cgagctgaac
     7741 cagctgacct acggccagaa gctgaccggc gtgagcaggg acgaccccaa cgccgacacc
     7801 tcggttcacc gttacggcgg ggcgctcggc ggcccgctgt tcaagcggac gttcttctac
     7861 gcgaactacg aggggttgcg cgacaagacg atccagggtg gcggcaccat caccgtgccg
     7921 ggcgcggcgc tccgcgccgg cgacttctcg gcgtccacga tcacggtgag ggatcctgaa
     7981 accggggagc cgttcccgga caaccagatt cctgccgagc gcatcagccc gatcgcccag
     8041 aacatcatga acatgttctg gccgatgccg aaccggtcgg tcgtggcgag cgggatgggc
     8101 accttccagc agtacgtgcc cgtcaagcgg aagcgcgagc ggttcgacct ccggctcgat
     8161 cacgaggcgg gaaccggcaa ccagttcttc gcgcggggca gttaccagta ccggaacccg
     8221 acccggatgc agtgggaggc cggggctgcc ctgacgcagc tgccgctggt caagagcgcg
     8281 atgaacacgt acgcgctggt cggcgggtgg acctcgattc tcagcagccg gctcgtgaac
     8341 gaagtgcggg tcgggtacaa cttcgacggg agcgagcgga agagcacctg gcaggcgggc
     8401 gacatcgccg cgaagatggg catcgagctg ccgccgagcg tctcccccga cacccccggc
     8461 ttcccctcga tcaacttcgt tgccggttcg aaccgtcccg ggaacatcgc cgacggc
//
LOCUS       DAOPQX010000146         8476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9345_length_8476_cov_4.973875, whole genome shotgun sequence.
ACCESSION   DAOPQX010000146 DAOPQX010000000
VERSION     DAOPQX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9345_length_8476_cov_4.973875"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PKK95_09115"
     CDS             <1..1042
                     /locus_tag="PKK95_09115"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOC18415.1"
                     /translation="QEFIQNLKIDLYPEEVYTFTPKGQVKPLPRGATPVDFAYSIHTD
                     VGNQCVGARVNGRMVPLRTPLKNGDIVEIVTQAKHRPSRDWLNFAVTNRARNKIKHYL
                     EGEEKARAVELGRRVLEKEVRRFDLNAKALLDEAALEKAAAASGVQKVDDMLAAVGFG
                     RLQARQVLDKLVPAEQLQEPSPSTGVVSAVRRALSRGGDRIKVRGADDVLVVRARCCN
                     PIQGEKIVGYITRGKGVSVHSAACPNVANLLYASDRRIDVEWDLGGEPGRYGVDLKLT
                     VEDRKGLLAEISAKIADINTNIISVEARSEDGRLDHIAMTVEIHDVKHLQKVLKSLRR
                     VNGVLDVERAAG"
     gene            complement(1078..1464)
                     /locus_tag="PKK95_09120"
     CDS             complement(1078..1464)
                     /locus_tag="PKK95_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOC18416.1"
                     /translation="MAQAVSTALAPAAIGPYSQAVRAGDFLFVSGQIPLDPSSGAVVA
                     GDVSAQTRRVLENMGEILKAAGATFAGVVKTTVYLADLNDFEGMNQVYAGFFGSPAPA
                     RAAVQVSRLPRDVRVEIEAIVYLGAN"
     gene            complement(1467..1658)
                     /gene="rpmB"
                     /locus_tag="PKK95_09125"
     CDS             complement(1467..1658)
                     /gene="rpmB"
                     /locus_tag="PKK95_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOC18417.1"
                     /translation="MAKRCEICGKGPVVGRNISHAHNVTSRRFEPNLQTVRASVNGGV
                     KRLRVCTRCLRSGKVIKAA"
     gene            complement(1768..2226)
                     /locus_tag="PKK95_09130"
     CDS             complement(1768..2226)
                     /locus_tag="PKK95_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18418.1"
                     /translation="MAAMLEGLDERQQQLCWLVLQPVLAFADPFLTPTDADVAEAAAA
                     LAATYETAGKGLIYEHRAGSLVGQRLGADIRRVLAEAGIDSSRTLERDAAVVLRRVEA
                     AARAAGRQGSAGPASLLEAVRRVSREAEREARTAGLQAGPAGAGPSLIVP"
     gene            2859..3317
                     /locus_tag="PKK95_09135"
     CDS             2859..3317
                     /locus_tag="PKK95_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOC18419.1"
                     /translation="MEATKKKKRVEAGKARYEELKEMLEERRRQILSQVHEKIRDVRT
                     ENVSGKVNTVLDPGESSEAGIQEDIEFALIQMKAETLHKINEALQRLEEGAYGNCFEC
                     GEEISRQRLRALPFAVRCIECEEARETAQKRERMMSQRSANSALFFDMSS"
     gene            3412..5874
                     /gene="lon"
                     /locus_tag="PKK95_09140"
     CDS             3412..5874
                     /gene="lon"
                     /locus_tag="PKK95_09140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOC18420.1"
                     /translation="MSEDPETPIFDEAGGERALSIPPELPVLPLRDTVLFPNSFMPLA
                     VARESSIRLIDEAISGGKLIAVFTQRDASVEEPGQDDLHRIGTATHVHKMFKLPDGSL
                     RLIVQGLARLRMVKLVSLKPYLRAEVSAAGEDLREEDRLEIDALQRNIKTNFQQVVSL
                     SPLLSDDLQTLAINITDPGKLADFIASSLTTIATSAKQEVLETLDVRARMDLLNRILI
                     KELEVLELGSKIQSQVQSEVGKNQRDYFLREQLKAIQKELGEGDDQAKEIEELRGKIE
                     SAGMPEPVKKEALRELDRLSKMPVAAAEYTVSRTYLDWLVALPWNRRTDEVIDLKRTK
                     DVLDADHSDLVRAKDRILEYLAVRKLNPEVKGPILCFVGPPGVGKTSLAKSIATSLDR
                     KFVRISLGGMRDEAEIRGHRRTYIGALPGQIIQGLRRAESKNPVFILDEIDKLGADFR
                     GDPASALLEVLDPEQNNTFRDHYLDVPFDLSEVLFITTANILDPVPPALRDRMEVLEL
                     PGYTEEEKLKIAQEHLVGKQVQNHGLKPEQIEFTEGALRAIIRGYTREAGVRNLEREI
                     AAVCRKAARRRAEGLEEPLTVTPDVVVEMLGAPKVLEEEMEERTKNPGVAIGLAWTPQ
                     GGEVLFVEASRMPGTGSLTLTGHLGDVMKESARAALSWLRANATAFGIDPNFYRAAEI
                     HLHVPSGAIPKDGPSAGVTMVTALCSELTGRPVRGDLAMTGEITLSGRVLPVGGIKEK
                     VLAARRVGIREVIVPARNEKNIVEDLNEELRREMKVHLVNTIQEVLDLALLSAVEAPR
                     PIAEPVAGAPAPEAGVVHTVDG"
     gene            complement(5883..6425)
                     /locus_tag="PKK95_09145"
     CDS             complement(5883..6425)
                     /locus_tag="PKK95_09145"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOC18421.1"
                     /translation="MTKTGRQLSGETVYDGKIFRVERDRVRLPNGREATLEIVRHRGS
                     VVLLPIPEPGRVILVRQYRYAVGEHLWELAAGSLEPGEDPAEGAARECEEETGWVPAD
                     AELVGRFYPTPGYCDELMNFYRLTGLARRTEGTAAIPDDDEDLEVRSFTLEELTQMVR
                     AGEIRDLKTAVGLGLLDRTL"
     gene            complement(6556..7509)
                     /locus_tag="PKK95_09150"
     CDS             complement(6556..7509)
                     /locus_tag="PKK95_09150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOC18422.1"
                     /translation="MERHEFTVGAEDAGLRLDVYLAGLLPTHSRSQLQRLIKEGLVRV
                     GGGAAKPNQPVKTGQAVVVEIPPPVAATPRPQDLALPILFQDEDVLVIDKPAGMVVHP
                     GAGHAEGTLVNALLFHADDLSGVGGEERPGIVHRLDRGTSGLMVVAKNDLAHRELARQ
                     FQEREVEKEYVALVWGEVHAGRRIDLPIGRDPVHREKMSTRARRARTAVTRVTAAHDL
                     RGVTLVHVAIHTGRTHQIRVHLSAIGHPVVGDRTYGGVRRHMAASLRPVERLERPFLH
                     AGRLVFAHPRDGRRLEFEAPLPEDLQDVLDELLERAAGHHP"
     gene            complement(7515..8060)
                     /gene="lspA"
                     /locus_tag="PKK95_09155"
     CDS             complement(7515..8060)
                     /gene="lspA"
                     /locus_tag="PKK95_09155"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOC18423.1"
                     /translation="MTPEDSHPAGSTGVPTQRGGGLSPLTFWIAGAIVVVDQATKALV
                     RSRLPLHASETVIPGFLDLTHVRNTGAAFGILGAVDFPFKPAVIALLATAALVAVAVF
                     AAQLSPHQKFARIGLALILGGAVGNLIDRVTAGYVLDFVDVYWRDWHFWAFNVADAAI
                     SVGVAGMLLDLVLPGRAHAAP"
     gene            complement(8057..>8476)
                     /locus_tag="PKK95_09160"
     CDS             complement(8057..>8476)
                     /locus_tag="PKK95_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18424.1"
                     /translation="TFPDPSALADPELASRWERLLEVREAVNVEIEKLRKAKVVGKSL
                     EASVTLKARGPLLELLRRHLDDLPTLFIVSEVTLDEAAEGDDATLPVVTVRRVGGERC
                     ERCWRYVPEVGGPAVGGHEGREGLCGRCLQALNEAGL"
BASE COUNT         1413 a         2949 c         2931 g         1183 t
ORIGIN      
        1 gcaggagttc atccagaacc tgaagatcga cctctacccc gaggaggtct acaccttcac
       61 gccgaagggg caggtgaagc cgctgccgcg cggcgcgacg ccggtcgact tcgcgtactc
      121 catccacacc gacgtcggca accagtgcgt cggcgcccgg gtcaacggcc ggatggtccc
      181 gctccgcacg ccgctcaaga acggcgacat cgtcgagatc gtcacgcagg cgaagcacag
      241 gcccagccgc gactggctga acttcgccgt caccaaccgc gcccggaaca agatcaagca
      301 ctacctggag ggcgaggaga aggcccgcgc cgtcgagctg ggccggcgcg tgctcgagaa
      361 agaggtgcgc cgcttcgacc tgaacgccaa ggcgctgctc gacgaggcgg cactcgagaa
      421 ggccgcggcg gcctcgggcg tccagaaggt cgacgacatg ctcgccgccg tcgggttcgg
      481 acgcctgcag gcccggcagg ttctcgacaa gctggtgccc gccgagcagc tgcaggagcc
      541 ctcgccctcc accggcgtgg tgtcggccgt ccgccgcgcg ctctcgaggg gcggcgaccg
      601 catcaaggtc cggggcgctg acgacgtcct ggtggtccgc gcgcggtgct gcaacccgat
      661 ccagggcgag aagatcgtcg gctacatcac gcgcggcaag ggagtgtcgg tgcactcggc
      721 ggcctgcccc aacgtcgcga acctgctcta cgcctccgac cgccggatcg acgtcgagtg
      781 ggacctgggc ggcgagccgg gccggtacgg cgtcgacctg aagctcacgg tcgaagaccg
      841 gaagggactg ctggccgaga tcagcgcgaa gatcgccgac atcaacacca acatcatcag
      901 cgtcgaggcg cgcagcgagg acggccgtct cgaccacatc gcgatgactg tcgagatcca
      961 cgacgtcaag cacctgcaga aggtgctcaa gtcgctgcgc cgggtgaacg gggtgctgga
     1021 cgtggagcgc gccgccggct gacgccgggg gccggagcag gaagggaatt ttcgggttca
     1081 gttcgccccg aggtagacga tcgcctcgat ctccacccgg acatcacgcg gcaagcgcga
     1141 gacctggacc gccgcgcgcg cgggcgcggg cgagccgaag aaaccggcgt acacctggtt
     1201 catcccctcg aagtcgttca ggtcggcgag gtacacggtc gtcttgacca cgcccgcgaa
     1261 ggtggccccg gccgccttca ggatctcccc catgttctcg agcacccggc gagtctgagc
     1321 cgagacgtcg cccgccacca ccgccccgct cgatgggtcg aggggaatct ggccagagac
     1381 gaacaggaag tcgccggccc tgacggcctg ggagtacggc ccgatggccg ccggggcgag
     1441 agcggtcgag acggcctgcg ccacggctag gccgccttga tcaccttgcc cgagcggagg
     1501 cagcgcgtgc agacgcgcag ccgcttcacg ccgccgttca ccgacgcgcg caccgtctgg
     1561 aggttcggct cgaaccgccg ggaggtcacg ttgtgcgcgt ggctgatgtt gcgcccgacg
     1621 accgggccct tgccgcaaat ctcacaccgc ttcgccatgg ctacccgccc gagaaggagt
     1681 cgacaggttg gggttccacc cggaacacga aaccatgagt ataccacgcc ccagccaggt
     1741 ttccgctgcc cggcaccggt cgcccgatca gggaacgatg aggctcgggc cggcgccggc
     1801 agggccagcc tgaagtccgg ccgtccgcgc ttcgcgctcc gcctcgcgcg acactcgccg
     1861 gacggcctcg agcaggctgg ccgggcccgc cgagccctgg cgcccggcgg cccgggcggc
     1921 cgcctcgacg cgccgaagca cgacggcggc gtcgcgctcc agcgtccgcg acgagtcgat
     1981 gccggcctcg gccaacaccc gcctgatgtc ggctcccagc cgctgcccga cgagcgagcc
     2041 ggcgcggtgc tcgtagatga gccccttgcc cgccgtctcg taggtggccg ccagcgcggc
     2101 ggccgcctcg gccacgtcgg cgtccgtggg agtgaggaac gggtcggcga acgccagcac
     2161 gggttgcagg accagccagc agagctgctg ctgccgctcg tcgagcccct ccagcatcgc
     2221 ggccatcgag gcggtgtcgc gctcgtgctg ccggcggacg gaagcgggcg gatgggcacg
     2281 ggaagaggcg aggaacgggc aatccggggg gcaggcaatc tcggtcaggc gcttggtccc
     2341 gcagcacacg gggcagatcc gagccccctt ggcggggcag aagcggcgcg gcgcgcgctc
     2401 gcggcacaga ggacatgacg acatggcgtc agcttcgcat acgccggggt gccgggcaag
     2461 gcaggaaggc aaacttgcca caccggaggg tcagacttgt tgcagagaga ggacaggctg
     2521 aacgcggccg gcggcttgga agccgtcttg cgaccggcgg cgtctaaaaa ggcgacgggg
     2581 tcgaagccga agcgtcagcg gaccggcaga ggacggtaat tagctgaacc gtcagccttt
     2641 cgcggtccgc cggatgggat gagctgttcg cgagcgtgtg aagacgctac acgcccttcc
     2701 gggagtcgct gtaaggtttt cggcaaaccg gcggcgtggg cggagataaa tcgagaaaac
     2761 gacgatctgc acgatatggc cgtggatttg catggtgcgt cgtgctacta tgtgccctcc
     2821 tgcgaaggca gggccgacgc ggaaggagcg tcagcgccat ggaagcgacc aagaagaaga
     2881 agagagttga agccggcaag gcgcggtatg aagagctgaa ggagatgctg gaggagcgcc
     2941 gccgtcagat cctcagccag gtccatgaga agatccgcga cgtccggacc gagaacgtgt
     3001 ccggcaaggt caatacggtc ctggatcccg gcgagagttc cgaggccggg attcaagagg
     3061 acatcgagtt cgccctcatc cagatgaagg ccgagacgct gcacaagatc aacgaggcgc
     3121 ttcagcggct cgaggagggc gcgtacggca actgcttcga gtgcggcgag gagatctccc
     3181 gccagcgcct gcgggcgctg ccgttcgcgg tccggtgcat cgagtgcgag gaggcgcgcg
     3241 aaacggcgca gaagcgcgag cgcatgatgt cgcagaggag cgcgaactcg gcgctcttct
     3301 tcgacatgtc gagctgacga tcgccgtcaa ccgggcgggc cttccggccc gcccgtcccg
     3361 accgccttcc tcccgatatt ctccaacccg tatcccaaac gagagcgtgg catgagcgag
     3421 gatcccgaaa ccccgatatt cgacgaggcc gggggggagc gggccctctc gattcccccg
     3481 gaactgccgg ttctgccgct gcgggacacc gtcctctttc cgaattcgtt catgccgctg
     3541 gctgtcgccc gcgagagctc gattcgcctc atcgacgaag cgatcagcgg cggcaagctg
     3601 attgccgtct tcacgcagcg cgacgccagc gtcgaggagc cggggcagga cgacctgcac
     3661 cgcatcggca ccgcgacgca cgtccacaag atgttcaagc tgcccgacgg cagcctgcgg
     3721 ctgatcgtgc aggggctcgc gcggctccgg atggtcaagc tcgtctcgct caagccctac
     3781 ctgcgcgccg aggtgtcggc cgccggcgag gacctgcgcg aggaggaccg gctcgagatc
     3841 gacgccctcc agcggaacat caagacgaac ttccagcagg tcgtctcgct gtccccgctg
     3901 ctctccgacg acctccagac gctggccatc aacatcaccg acccgggcaa gctcgccgac
     3961 ttcatcgcct cgagtctgac caccatcgcc acctcggcca agcaggaggt gctcgagacg
     4021 ctagacgtcc gcgcgcgcat ggacctgctc aaccggatcc tgatcaagga gctcgaggtg
     4081 ctcgagctgg ggtcgaagat ccagtcgcag gtgcagtcgg aggtgggcaa gaaccagcgc
     4141 gactacttcc tccgcgagca gctcaaggcg atccagaagg agctgggcga gggcgacgac
     4201 caggccaagg agatcgagga gctgcggggc aagatcgaat cggccggcat gcccgaaccg
     4261 gtgaagaagg aggcgctgcg cgagctcgac cgcctctcga agatgccggt ggcggccgct
     4321 gagtacacgg tctcgcgcac ctacctcgac tggctcgtcg cgcttccctg gaaccgcagg
     4381 accgacgagg tgatcgacct caagcgcacg aaggacgtcc tcgacgccga tcactccgac
     4441 ctcgtgcgcg cgaaggaccg catcctcgag tacctcgcgg tccgcaagct gaaccccgag
     4501 gtgaaaggcc cgatcctctg cttcgtcggc ccccccggcg tcggcaagac ctcgctcgcc
     4561 aagtcgatcg ccacctcgct cgaccggaag ttcgtccgca tctcgctcgg cggcatgcgc
     4621 gacgaggccg agatccgcgg gcaccgccgc acctacatcg gcgccctccc ggggcagatc
     4681 atccaggggc tgcgccgggc cgagtcgaag aacccggtgt tcatcctcga cgagatcgac
     4741 aagctcggcg ccgacttccg gggcgacccc gcctcggcgc tgctcgaggt gctcgatccc
     4801 gagcagaaca acacgttccg cgaccactac ctcgacgtcc ccttcgatct ctccgaggtc
     4861 ctgttcatca ccaccgcgaa catcctcgac ccggtgcccc cggcgctccg cgaccgcatg
     4921 gaggtcctcg aactccccgg ctacacggag gaggagaagc tcaagatcgc gcaggagcac
     4981 ctggtcggca agcaggtcca gaaccacggg ctgaagccgg agcagatcga gttcaccgag
     5041 ggggcgcttc gcgccatcat ccgcggctac acccgcgagg ccggcgtccg gaacctggag
     5101 cgcgagatcg ccgcggtctg ccgcaaggcc gcccggcgcc gtgccgaggg gctggaggaa
     5161 ccgctcacgg ttaccccgga cgtggtggtc gagatgctcg gcgcgccgaa ggtcctcgag
     5221 gaggagatgg aggagcgcac caagaacccc ggggtcgcca tcggcctcgc gtggaccccg
     5281 caggggggcg aggtgctctt cgtcgaggcc tcgcgcatgc cgggaaccgg cagcctgacg
     5341 ctgaccgggc acctcggcga cgtcatgaag gagtcggcca gggcggcgct gtcgtggctg
     5401 cgcgccaacg ccaccgcctt cggcatcgac cccaacttct accgggccgc cgagatccac
     5461 ctgcacgtgc cgtcgggcgc gatcccgaag gacggcccct cggccggcgt cacgatggtg
     5521 accgccctct gctccgagct gacggggcgc ccggtgcggg gcgatctcgc gatgaccggc
     5581 gaaatcaccc tgtcgggccg ggtgctgccc gtcggcggca tcaaggagaa ggtgctcgcg
     5641 gctcgccgcg tcgggattcg agaggtgatc gttcccgcgc gcaacgagaa gaacatcgtc
     5701 gaggacctga acgaggagct gcggcgcgag atgaaggtgc acctggtcaa cacgatccag
     5761 gaggtgctcg acctcgcgct gctgtcggcc gtcgaagcgc cccgcccgat cgcagagccg
     5821 gtggccggcg ccccggcccc cgaggcgggg gtggtgcaca ccgtcgatgg atagcgacgc
     5881 gattacaggg ttctgtcgag cagccccagc cccaccgccg tcttcaggtc ccggatctcg
     5941 ccggcgcgga ccatctgcgt cagctcctcg agcgtgaagc tgcgcacctc caggtcctcg
     6001 tcgtcgtcgg ggatcgcggc cgtgccctcg gtccgccggg cgagaccggt gaggcggtag
     6061 aagttcatca gctcgtcgca gtaccccggc gtggggtaga agcgccccac cagctccgcg
     6121 tcagcgggca cccagccggt ttcctcctcg cactcgcgtg ctgccccctc cgccgggtct
     6181 tcgcccggct cgaggctgcc cgccgccagc tcccagaggt gctcgccgac cgcgtagcgg
     6241 tactgccgca ccaggattac gcggccgggc tcgggaatcg gcagcagcac gaccgaaccg
     6301 cgatggcgca cgatctcgag cgtcgcctcc cggccgttcg gcagccgcac ccggtcgcgt
     6361 tccacccgga agatcttccc gtcatacacc gtctcgccgc tcaattggcg gccggtcttc
     6421 gtcacgatgc cctcccgttc gtcgtagagc ggcccttcgg gtttcgtcgt agagcagccc
     6481 ttcagggctg cctggtcctt gcctgccggt gatgagcagg gctaaagccc cgctctacgg
     6541 gtcatgagcc ctgctctacg gatgatgacc ggccgcccgt tccaggagct cgtccagcac
     6601 gtcctgcagg tcttccggca gcggcgcctc gaactcgagc cggcggccat cgcgcggatg
     6661 cgcgaaaacc aggcggccgg cgtggaggaa cgggcgctcg aggcgctcga ccgggcgcag
     6721 ggaggctgcc atgtggcggc ggacgccccc gtaggttcga tcgcccacga cagggtgccc
     6781 gatggcgctc aggtggacgc gaatctgatg ggtgcgcccg gtgtggatgg cgacgtggac
     6841 gagcgtcacc ccgcgaaggt cgtgcgccgc ggtgacgcgc gtgaccgcag tgcgtgcgcg
     6901 ccgggcgcgc gtggacatct tctcgcggtg gacggggtct cgcccgatcg gcagatcgat
     6961 ccgccgcccc gcgtgcacct cgccccacac gagcgccacg tactccttct ctacctcacg
     7021 ttcctggaac tgccgtgcca gctcgcggtg ggcgaggtcg ttcttggcga cgaccatcag
     7081 ccccgacgtt ccccgatcca gccggtggac gatgccgggg cgctcttccc ctccgacgcc
     7141 gctcaagtcg tcggcgtgga agaggagggc gttcaccagc gttccctcgg cgtgcccggc
     7201 acccggatga accaccatgc cggcgggctt gtcgatcacg agaacgtcct cgtcctggaa
     7261 gaggatcggc agcgccaggt cctggggccg gggcgtcgcg gccacgggcg gagggatctc
     7321 gaccacgact gcctgcccgg ttttcacagg ctggttcggc ttcgcggcgc ccccgccgac
     7381 cctgaccaat ccctccttga tgagacgctg gagctgggac cgcgagtggg tgggaaggag
     7441 ccccgcgagg tacacgtcga gccggaggcc ggcgtcctcc gcacccacgg tgaactcgtg
     7501 gcgttccatg acgctcaggg cgcggcgtgc gctcgccccg gcaggaccag gtcgagcagc
     7561 atcccggcga cgccgaccga gatggccgcg tcggcgacgt tgaaggccca gaagtgccag
     7621 tcgcgccagt agacgtccac gaagtcgagg acgtagcccg ctgtcacccg atcgatcagg
     7681 ttgccgaccg ccccgccgag gatgagggcg aggccgatcc gggcgaactt ctggtgcggg
     7741 gagagctggg ccgcgaacac ggcgacggcg accagcgccg ccgtcgcgag cagcgcgatg
     7801 actgccggct tgaacgggaa gtcgacggcg cccaggatcc cgaaggcggc ccctgtgttc
     7861 cgaacgtgcg tgaggtcgag gaatccgggg atgaccgtct cgctggcgtg aagcggcagc
     7921 cggctgcgca cgagtgcctt ggtcgcctgg tccacgacga cgatcgcgcc ggcgatccag
     7981 aaggtgagcg gcgagaggcc gcctccgcgc tgggtcggca cgccggtgga accggcgggg
     8041 tgtgagtcct cgggcgtcac agtcccgcct cgttcagggc ctggaggcaa cggccgcaca
     8101 ggccctcgcg cccctcgtgc ccgccaaccg cgggcccgcc gacctcgggc acgtaccgcc
     8161 agcaccgctc gcagcgctcg ccgccgaccc ggcgcacggt gacgacgggc agcgttgcgt
     8221 cgtcgccctc cgcggcctcg tcgagcgtca cttccgacac gatgaacagc gtcggcagat
     8281 cgtcgaggtg gcggcgcagc agctcgagca ggggcccgcg cgccttcagt gtcaccgagg
     8341 cttcgagtga cttgccgacc accttcgcct ttcgcagctt ttcgatctcg acgttcaccg
     8401 cctcgcgcac ctccagcagc cgctcccacc gcgaagccag ctccgggtct gcgagcgcgg
     8461 acgggtctgg gaaggt
//
LOCUS       DAOPQX010000147         8447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9382_length_8447_cov_5.020377, whole genome shotgun sequence.
ACCESSION   DAOPQX010000147 DAOPQX010000000
VERSION     DAOPQX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9382_length_8447_cov_5.020377"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PKK95_09165"
     CDS             complement(<1..688)
                     /locus_tag="PKK95_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC18425.1"
                     /translation="MIRIGRQVLVLAIIGAAAAAVAGCGSASESAASAAPAAGKPPVA
                     VGVATVAATDLQDSVEVVGSLEPKFSAEVKSEVSGIVNAVYVTEWVPVRRGARLARLD
                     TTETEAGIKALEAMEAQARVNEARARREHERALQLKQYGLITPQNLDDAKTAVDAAEA
                     GTAAARAQVQAAQARLAKSFITAPIDGVIALRAVNVGDRVENMGGGGLPLFRIVDNRL
                     LDLTVSVPSSR"
     gene            complement(685..1326)
                     /locus_tag="PKK95_09170"
     CDS             complement(685..1326)
                     /locus_tag="PKK95_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOC18426.1"
                     /translation="MPPPQAAVPPGERDEHTRSRLLRAAVQVFNKKGYSAASVREIVE
                     LANVTKPALYYHFGSKEGVLQAILREGLEEFRATAAKAIEMPGTTRERLTRLCEDLFG
                     LFMENVPVIQVVHRAFHDPAGNPPDFDLVEVERALGAALQAIVEAGVASGELRSVRSD
                     DVALAVIGVIGSAASRHLHPSLEPVGPERLRRVLDLVFDGVFKERREQGDERP"
     gene            1585..4086
                     /locus_tag="PKK95_09175"
     CDS             1585..4086
                     /locus_tag="PKK95_09175"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOC18427.1"
                     /translation="MPQQDVELMDRGQQASPHAVDEAVLDAFRRWGYLEANLDPLGFL
                     QPRPHPELRGDSDAARQARAWYCGPIGAEFMHIPDPERRRWVQERMEAPAPAIDQQAV
                     FERLLRAEVFEVVLHGRYPGSKRFSLEGVTALIPLLDEVLETAAAQGLREALIGMSHR
                     GRLNVMVHIVGRPVRDVYAGFEDPDPKSVLGSGDVKYHMGATGTYRTRAGTELRVKLV
                     SNPSHLEAVYPVALGRARARQTREGEGARRRVMPIVLHGDAAFAGQGITAEALNIADL
                     PGYTVGGAVHVVVNNLLGFTTPDRELHASPFATDVARRLMVPIFHVNGENLEAVVRAA
                     RLATEFRYTFGTDAVIDMIGYRRHGHSEVDDPTITQPLMYKAIKAHPALWEIHARQAG
                     LDVGDAPARVRDEYSRAQEEASQLDSRVPLAQLPDYWSPYHGGCHDRKDELDTGVPLE
                     TLREVTAKLTSWPDDFHVHPKVRKLLEQRAAMVEGKHRLDYGMAESLAFATLLAEGHP
                     VRLTGQDSERGTFSHRHAVLIDVETERKYMPLGHLAPNQGACEIYNSTLSEAAILGFE
                     YGYSRDYPEALVLWEAQFGDFANNAQVVIDQFLSAGEDKWGLLSGLVLLLPHGYEGQG
                     PEHSSARIERFLQLAAEDNLQICQPSTAAQYFHLLRRQALRKWRKPLVVFTPKSMLRH
                     PSSSSPVEELTRGRFRTVLDDEQAPAGARRVLVASGKILHELRAERQRRGDTATAIIG
                     LEQLYPFPATPLSAALGRHPEAREVVWVQEEPKNNGAHFYVVPRLRAIVRHPGVRSVK
                     RRASASPATGSGKAHLLEQQALLALAFTSSGETEE"
     gene            4182..4742
                     /locus_tag="PKK95_09180"
     CDS             4182..4742
                     /locus_tag="PKK95_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOC18428.1"
                     /translation="MLGWLQVSQFTVFLALAAAGFTVLLISLVFGELFEHIGFDHDAD
                     HDLSHGGPGFFSTRVMSVFVTTFGGAGALATYYGLSPLPASLIGFLTGIVFASLIYAF
                     ARFLYGQQATSEVRAAELVGRHCRVVVAIPARGVGQVRCQVGEELVDKIARSSDGLAI
                     PENTAVTISEVLGEMVVVKAASAEQG"
     gene            4817..6370
                     /locus_tag="PKK95_09185"
     CDS             4817..6370
                     /locus_tag="PKK95_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOC18429.1"
                     /translation="MLGEILLIPIVVVVVALALLIAGLLFARNYIKVSPNVVAVLSGR
                     KRKLPDGRVVGYRMVKGGAALRIPLLEKVEYFWLNVMTIPLEIRRAYTLKGVPVSVKA
                     VANVKIRSDDTSLQAAAERFLGMTRDQIQQVIFQTLEGHLRSILGTLTVEEINNDRQS
                     FAQKLTSEAAVDLEKMGLGVDVLTIQEISDEEDYLNALGKRRTAEVKRDATIGEAEAT
                     RDAKIKSALALQEGEKARFSAEAEIALAQRDFMIRQAQYQAEVETQKATAAQAGPLSD
                     AKARQGVVAEEVKVERQRTQEMISVQEQEVLRRQKELEATVIKPAEADRQAAIVRAEA
                     AKQASVLEAEGRRAALIALAEAEQEKLRKEGVGRAAAVEAEGHAEAAKIEAIGLAQAK
                     AIEAQGVAEATAILRKAEAWQQFNEAAKLQTILEKLPAIIQASSGVFGAVAAPLGNID
                     KLVVIDQGGAANGTSGGVERLAKVSPALVFNLLQQLQALGLDVPTVLQQLGVQSAATA
                     QSSTVLPPR"
     gene            complement(6400..8133)
                     /locus_tag="PKK95_09190"
     CDS             complement(6400..8133)
                     /locus_tag="PKK95_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOC18430.1"
                     /translation="MKRILTPEREALLKDERRVLGDLRAALARHGTDPEAERTLERSI
                     AQLEELFLLVVVGEFNAGKSAFVNALLGREVLQEGVTPTTTEVEVLRYGESVSRVAIE
                     EHLYAVTAPVEMLRDIQIVDTPGTNAIMREHEVITSRFVPRADLVLFVTSADRPFTET
                     ERAFLERIRDWGKKVVVAINKVDILEREEQVNEVRDFVERHAAALLGTTPEVFAVGSR
                     AALRAKLGEPRFWDGSRFEALERYVTETLDEESRLRLKLLSPLGVASHLLEIQRSLAA
                     SRLALLKDDLEALGEVDRQLEVYRGDMARDVAFRMADIEKTLVDMEQRGHHYFDETLR
                     IGRVLDLLNRSRIQQEFEREVVADAPQLVERKVGELIDWLVDADFRQWQAVTDRLAER
                     RRQHRDRILGDQSSSAFHADRSRLIDSVGREAQRVVEGYDKAREAEALADGARNAVAA
                     SAAVGAGALGLGTVVSLAATSAAADVTGMLMAGVIAALGFFILPARRRRAKEEMRAKV
                     TALRAKLVGALRTEFEREMTRGRQRIEEAIAPYSRFVRAEQAKLTAMQGDLEAIADDI
                     RALRVRIEAAS"
     gene            complement(8150..>8447)
                     /locus_tag="PKK95_09195"
     CDS             complement(8150..>8447)
                     /locus_tag="PKK95_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18431.1"
                     /translation="LVEARRGVPPSRFGEIATACSRAIVERTSLVPDEVVVVAAGSLP
                     RTSSGKLRRGEALRQYNAGELRAPAPVTAPRLAGAAARSALSFLRARLFPRREP"
BASE COUNT         1310 a         3013 c         2924 g         1200 t
ORIGIN      
        1 ggcgcgacga gggcaccgag acggtcaggt cgagcagccg gttgtccacg atccggaaca
       61 gcggaaggcc gccgccgccc atgttctcga cccggtcgcc gacgttgacg gcgcgcaggg
      121 cgatgacgcc gtcgatcggc gcggtgatga acgacttggc cagcctggcc tgcgccgcct
      181 gcacctgcgc ccgcgccgcc gccgtaccgg cctccgccgc gtcgacggcc gtcttcgcat
      241 cgtcgaggtt ctgcggcgtg atcaacccgt actgtttcag ctgcagcgcg cgctcgtgct
      301 cccggcgcgc ccgcgcctcg ttgacgcgcg cctgcgcctc catcgcctcg agcgccttga
      361 tccccgcctc tgtctcggtc gtgtcgagcc gcgcgagccg cgcgccccgc cgcaccggca
      421 cccactcggt gacgtagacc gcgttgacga tgcccgagac ctccgatttc acttcggccg
      481 agaacttggg ctccagcgag ccgaccacct ccaccgagtc ctggagatcg gtcgctgcga
      541 cggtcgcgac tcccaccgcc acgggaggct tgccggccgc cggggcggcc gacgctgccg
      601 actctgatgc cgaaccgcag cccgccaccg cggccgccgc ggcaccgatg atcgccagga
      661 cgaggacttg acgcccgatc cgtatcatgg tctttcgtct ccttgctcgc gccgttcctt
      721 gaacacgccg tcgaacacca gatcgagaac ccggcgaagc cgctcggggc ccaccggctc
      781 gaggctcggg tggagatgcc gcgaggcggc cgacccgatc acgccaatga ccgcgagggc
      841 cacgtcgtcg gagcggaccg atcgcagctc ccctgacgcc acccccgctt cgacgattgc
      901 ctgcagggcc gcgcccaggg cacgttcgac ctcgacgagg tcgaagtcgg gggggttgcc
      961 cgcagggtcg tgaaacgccc ggtgtacgac ctggatcacc ggcacgtttt ccatgaagag
     1021 gccgaacagg tcttcgcaca ggcgcgtcag ccgctcccgc gtcgtcccgg gcatctcgat
     1081 ggcctttgcg gccgtcgctc ggaactcctc gagaccctcg cgcagaatgg cctggagaac
     1141 tccctccttg ctcccgaagt ggtagtagag ggccggcttg gtgacgttcg ccaactcgac
     1201 gatttcgcgg accgatgccg cggagtaacc cttcttgttg aagacctgga ccgcggcccg
     1261 gagcaggcgg cttcgcgtgt gctcgtcccg ctctcccggg ggcacggcag cctgcggcgg
     1321 cggcaatcca ttgggaagcg tattcaaggt gacaactccg ggggctgtga ctacctaccg
     1381 gtcggtatgc attacctacc ggtcggtaac tataacagac ggagctcgac cctaacaagt
     1441 tcccggaagg aatcaggaat cgggattcgg gattcagggt attcgggttt ccgattcctg
     1501 aatcctgtgc cccggggtgg acaacccctg cacgatgcgt aagactgtcg atcgggattc
     1561 ctctctcaca ggaccaggtg agcgatgccg cagcaggacg tcgagctgat ggaccgtggc
     1621 cagcaggcca gcccccacgc ggtggacgag gccgtgctcg acgcgttcag gcggtggggg
     1681 tacctcgagg ccaacctcga cccgctcggg tttctccagc cgcggccgca ccccgagctg
     1741 cggggcgaca gtgacgccgc ccggcaggcc cgcgcgtggt actgcgggcc gatcggcgcc
     1801 gagttcatgc acatccccga ccccgagcgc cggcgttggg tgcaggagcg gatggaggcg
     1861 ccggcaccgg cgatcgacca gcaggcggtc ttcgagcgcc tgctgcgcgc cgaggtcttc
     1921 gaggtggtgc tgcacggccg gtatcccggc tcgaagcggt tctccctgga aggggtgacg
     1981 gcgctgatcc cgctgctcga cgaggtgctc gagacggcgg cggcccaggg gctgcgcgag
     2041 gcgctcatcg gcatgagcca ccgcggccgc ctcaacgtga tggtgcacat cgtcggccgg
     2101 ccagtgcgcg acgtctacgc cggtttcgag gatcccgacc cgaagagcgt gctgggaagc
     2161 ggcgacgtga agtaccacat gggggcgacc ggcacctacc ggacgcgcgc ggggaccgag
     2221 ctgcgggtga agctggtgtc gaacccgagc cacctcgagg cggtctaccc ggtcgcgctc
     2281 ggccgggcac gggccaggca gacccgcgag ggagagggcg cgcggcggcg cgtgatgccg
     2341 atcgtgctgc acggcgacgc ggcgttcgcc ggccagggga tcacggccga ggcgttgaac
     2401 atcgccgatc tcccgggcta cacggtgggc ggggcggtcc acgtggtggt gaacaacctg
     2461 ctcggcttca ccacgcccga ccgcgagctg cacgcctcgc ccttcgccac cgacgtcgcg
     2521 cgccggctga tggtgccgat cttccacgtg aacggcgaaa acctggaggc ggtggtccgg
     2581 gcggcgcggc tcgccaccga gttccgttac accttcggca ccgacgccgt gatcgacatg
     2641 atcggctaca ggcggcacgg ccacagcgag gtggacgacc ccaccatcac ccagccgctg
     2701 atgtacaagg cgatcaaggc gcatcccgcg ctgtgggaaa tccacgcccg gcaggcgggc
     2761 ctcgatgtgg gcgacgcccc ggcgcgggtg cgggacgagt acagccgcgc gcaggaggag
     2821 gccagccagc tcgactcgcg ggtcccgctg gcgcagctcc ccgactactg gtccccctac
     2881 cacggcggct gccacgacag gaaggacgag ctcgacaccg gcgtcccgct cgagaccctg
     2941 cgcgaggtga cggcgaaact gacctcgtgg ccggacgact tccacgtgca cccgaaggtt
     3001 cggaaactcc tcgagcagcg cgccgcgatg gtcgaaggga agcaccgcct cgactacggc
     3061 atggccgagt cgctggcgtt cgccaccctg ctggccgagg gccaccccgt ccgcctcacg
     3121 ggacaggaca gcgagcgcgg gactttcagc caccggcacg ccgtgctgat cgacgtcgag
     3181 accgagcgga agtacatgcc gctcgggcac ctggcgccga accagggggc ctgcgagatc
     3241 tacaactcga ccctctcgga agccgcgatc ctgggcttcg agtacggcta cagccgcgac
     3301 taccccgagg cgctcgtcct ctgggaggca cagttcggcg acttcgccaa caacgcgcag
     3361 gtggtcatcg accagttcct cagcgcgggc gaggacaagt ggggcctgct ctcggggctg
     3421 gtcctgctgc tgccgcacgg ctacgagggc caggggcccg agcactcgag cgcgcgcatc
     3481 gagcggttcc tgcagctggc cgccgaggac aacctgcaga tctgccagcc ctcgaccgcg
     3541 gcccagtact tccacctgct gcgccgccag gcgctgcgga agtggcgcaa gccgctggtc
     3601 gtcttcaccc ccaagagcat gctgcgccac ccctcgtcct cctcgcccgt cgaggagctg
     3661 acgcgcgggc gcttccggac ggtgctcgac gacgagcagg ccccggccgg ggcgcggcgg
     3721 gtgctcgtcg cctccgggaa gatccttcac gagctgcgcg ccgagcgcca gcggcgcggc
     3781 gacacggcga cggccatcat cggcctcgag cagctctacc ccttcccggc gacgccgctg
     3841 tcggccgccc tgggccgcca ccccgaggcc cgcgaggtcg tgtgggtgca ggaggaaccg
     3901 aagaacaacg gggcccactt ctatgtcgtg ccgcggctgc gggcgatcgt gcgccatccg
     3961 ggtgtccgct cggtgaagcg ccgcgcctcg gccagcccgg ccaccggctc gggcaaggcg
     4021 cacctgctcg agcagcaggc gctgctggca ctcgccttca cctcgagcgg ggagacggag
     4081 gaataggaat cgggagtcgg gagtcaggaa tcaggggagc ccccgaatcc cgaatcctga
     4141 atcccgaatc ctgataaagt gacgccaacc gaggagggcg aatgctcggc tggctgcagg
     4201 tttcgcagtt cacggtgttc ctcgcgctgg cggccgccgg cttcaccgtg ctgctgattt
     4261 cgctggtctt cggcgaactg ttcgagcaca tcgggttcga ccacgacgcc gatcacgacc
     4321 tctcccacgg cggcccgggc ttcttcagca cccgggtgat gagcgtcttc gtgacgacct
     4381 tcggcggggc cggcgccctg gccacctact acgggctctc cccgctgccc gcctcgctga
     4441 tcgggtttct gaccggcatc gtcttcgcct cgctcatcta cgcgttcgcc cggttcctct
     4501 acgggcagca ggccacctcc gaggtccgcg ccgccgagct cgtcgggcgc cactgccggg
     4561 tcgtggtcgc catccctgcc cggggcgtcg gacaagtgcg ctgccaggtc ggcgaggaac
     4621 tggtggacaa gatcgcccgc tcgtccgacg gtctcgcgat ccccgagaac accgccgtca
     4681 cgatttcgga ggtgctgggc gagatggtgg tggtgaaggc ggcgtcggcc gagcagggct
     4741 gacggcgggc tgccgggccg ccgaggaaac gcggcccggg cgcacaaggc aacaggaaga
     4801 atgtaagggg ctttccatgc tgggcgagat cctcctgatc ccgatcgtcg tggtggtcgt
     4861 cgcgctcgcg ttgctgatcg ccgggctgct cttcgcccgc aactacatca aggtctcccc
     4921 gaacgtcgtg gccgtgctca gcgggcgcaa gcgcaagctg cccgacgggc gggtggtggg
     4981 ctaccggatg gtgaaggggg gcgccgccct tcgcatcccg ctgctcgaga aggtggagta
     5041 cttctggctg aacgtgatga cgatcccgct ggagatccgg cgggcgtaca cgctcaaggg
     5101 cgtgcccgtg tcggtgaagg cggtggccaa cgtgaagatc cgcagcgacg acacctcgct
     5161 gcaggcggcc gccgaacggt tcctcgggat gacccgcgac cagatccagc aggtgatctt
     5221 ccagacgctc gaggggcacc tgcgctcgat cctgggcacg ctgaccgtcg aggagatcaa
     5281 caacgaccgc cagagcttcg cccagaagct gacgagcgag gcggcggtcg acctcgagaa
     5341 gatgggcctc ggcgtggacg tcctgacgat ccaggagatc agcgacgagg aggactacct
     5401 caacgcgctc ggcaagcggc gcacggccga ggtgaagcgc gacgcgacga tcggcgaggc
     5461 cgaggccacg cgcgacgcca agatcaagtc ggcgctggcg ctgcaggaag gcgagaaggc
     5521 acgcttctcg gccgaggcgg agatcgcgct ggcgcagcgc gacttcatga tccgccaggc
     5581 gcagtaccag gccgaggtcg agacccagaa ggccaccgcc gcgcaggccg gccccctctc
     5641 cgacgcgaag gcccgccagg gggtcgtcgc cgaggaggtg aaggtcgagc gccagcgcac
     5701 gcaggagatg atctcggtcc aggagcagga ggtcctccgg aggcagaagg aactcgaggc
     5761 gacggtcatc aagccggccg aggccgaccg ccaggcggcc atcgtgcgcg ccgaggccgc
     5821 caagcaggcc tcggtgctcg aggccgaggg acgccgggcg gcgctcatcg cgctggccga
     5881 ggccgagcag gagaagctgc gaaaggaagg cgtcggccgg gccgccgcgg tcgaggccga
     5941 ggggcacgcc gaggcggcga agatcgaggc gatcggcctg gcgcaagcca aggccatcga
     6001 ggcgcagggc gtcgccgagg cgaccgccat cctccgcaag gccgaggcgt ggcaacagtt
     6061 caacgaggcc gcgaagctcc agacgatcct cgagaagctc cccgcgatca tccaggcctc
     6121 gagcggcgtg ttcggcgcgg tggcggcgcc gctcggcaac atcgacaagc tggtggtgat
     6181 cgaccagggt ggggccgcca acgggacgtc gggcggtgtg gagcggctgg cgaaggtgag
     6241 cccggcgctc gtcttcaacc tgctgcagca actgcaggcg ctcggcctcg acgtgccgac
     6301 cgtcctgcag cagctcggcg tccagtccgc cgcgacggca cagtcatcga ccgtgctgcc
     6361 gccccgctaa ggacggtttg aacgtggcgt gcctcggcct cagctcgcgg cctcgatgcg
     6421 cacgcggagg gcgcggatgt cgtccgcaat cgcttccagg tcgccctgca tggcggtgag
     6481 cttcgcctgt tcggcgcgca cgaagcggga gtagggggcg atcgcctcct cgatccgctg
     6541 gcgcccccgc gtcatctcgc gctcgaactc ggtgcggagc gcgccgacca gcttcgcgcg
     6601 aagcgccgtc accttggcgc gcatctcctc tttcgcgcgc ctccgcctcg ccggcaggat
     6661 gaagaagccg agcgcggcaa tcacgccggc catcagcatt cccgtgacgt cggcggccgc
     6721 gctggtcgcg gcgagcgaga ccaccgtccc cagtcccagc gctcccgccc cgacagccgc
     6781 cgaggcggcc accgcgttgc gcgcgccgtc ggccagcgcc tcggcctcgc gggccttgtc
     6841 gtacccctcc accacgcgct gcgcctcgcg gcccaccgag tcgatcaggc gcgagcgatc
     6901 ggcgtggaac gctgacgacg actggtcgcc caggatgcga tcgcggtgct ggcgccgccg
     6961 ctcggccagc cggtcggtca ccgcctgcca ctgccggaaa tcggcgtcca cgagccagtc
     7021 gatcagctcg ccgaccttgc gctcgaccag ctgcggcgcg tcggcgacga cctcgcgctc
     7081 gaactcctgc tggatccggc tgcggttcaa caggtcgagg acacgcccga tccgcagcgt
     7141 ctcgtcgaag tagtggtgcc cgcgctgctc catgtccacc agcgtcttct cgatgtcggc
     7201 catccggaat gcgacgtcgc gcgccatgtc gccgcggtac acctcgagct ggcggtccac
     7261 ctcgccgagc gcctcgaggt cgtccttcag cagcgcgagc cgcgaggcgg ccagcgagcg
     7321 ctggatctcg agcaggtgcg acgccacgcc gagcgggctc agcagcttca gccgcagccg
     7381 gctctcctcg tcgagcgtct cggtcacgta gcgttcgagc gcctcgaagc ggctgccgtc
     7441 ccagaaccgc ggctcgccca gcttcgcgcg cagcgccgcc cgcgagccga ccgcgaagac
     7501 ttcgggcgtc gtgccgagca gcgccgccgc gtgccgctcc acgaagtcgc gcacctcgtt
     7561 gacctgctcc tcccgctcca ggatgtccac cttgttgatg gccaccacca ccttcttgcc
     7621 ccagtcgcgg atccgctcga ggaaggcccg ctcggtctcg gtgaacgggc ggtcggccga
     7681 ggtgacgaac agcaccaggt cggcccgcgg cacgaagcgc gaggtgatca cctcgtgctc
     7741 gcgcatgatg gcgttggtgc cgggggtgtc cacgatctgg atgtcgcgga gcatctcgac
     7801 cggcgccgtc accgcgtaga ggtgctcctc gatcgcgacg cgcgaaacgc tctcgccgta
     7861 cctgagcacc tcgacctcgg tcgtcgtcgg cgtgacgccc tcctgcagga cctcccgtcc
     7921 gagcagcgcg ttgacgaagg cgctcttgcc cgcgttgaac tcgcccacca ccacgagcag
     7981 gaacagctcc tcgagctgcg cgatggagcg ctcgagggtg cgctcggcct cggggtcggt
     8041 cccgtgccgt gccagcgcgg cacgcagatc ccccaggacg cgacgctcgt ccttcaacaa
     8101 cgcctcgcgc tcgggcgtca ggattcgctt cataagcacc tgggcgggct cacggctcac
     8161 gtcgagggaa cagacgcgct cgaaggaacg aaagcgccga gcgggcggcc gcacccgcca
     8221 gccgcggggc cgtcaccggg gccggcgccc gcagctctcc cgcgttgtac tgccgcagag
     8281 cttcaccccg ccgcagttta cccgaagacg tgcgcggcag cgagccggcc gccaccacca
     8341 cgacctcgtc gggaacgagc gacgtccgct cgacgatcgc gcgcgagcag gccgtggcga
     8401 tctcgccgaa ccgcgagggc ggaacgccgc gccgcgcctc gaccagc
//
LOCUS       DAOPQX010000148         8398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9463_length_8398_cov_4.010907, whole genome shotgun sequence.
ACCESSION   DAOPQX010000148 DAOPQX010000000
VERSION     DAOPQX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9463_length_8398_cov_4.010907"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PKK95_09200"
     CDS             complement(<1..1096)
                     /locus_tag="PKK95_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18432.1"
                     /translation="MSDLRQFRVTLTTPDGLVHDLTDRTDLDSFGQITEELEEDLGLL
                     THSDMDVEFFDEDGVVSGLLEGAVRGQVFELVVERETGLAEPQWKRVFAGVLDVPGSI
                     HQTEGAGTVSLQVFAYSKLLELHSAEAVRRTVEDRTGTVSAGSRTVTVSDTTDLAAGD
                     EITLDGGGESETQTIRTVDSSTQVTTLETWTNAFTAAPLTLETPYYRSQSVTTLAGRL
                     AAEAGLTDVHVDIAQQLAELPFPTDMNDTGLPSGSPAGLLERSSDIAVYAGGNRYDAA
                     DQQSGFTGAGADTVKCDWRPYALTEPATLRAASGSDTGGRAWDYAGGDYYELALVGSD
                     LKLRKNGVAIATVVTLNTYDQLYAYGLDWCP"
     gene            complement(1093..3417)
                     /locus_tag="PKK95_09205"
     CDS             complement(1093..3417)
                     /locus_tag="PKK95_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18433.1"
                     /translation="MAEKSVTLAGIVKMVLQDNGVLELKSRLDKLDKKLDETKKKMGG
                     LGDAGKQVGQYLAGAFALGTIAAFVKSSITEFAKLERGFNAIGFQMQRLGLDAKGSLP
                     EIKAGLQAIQDSGGPLVSETIPIFQKFIGITKDTDAALYATKLAADISEAGFMEAGAA
                     GAALAAIMQGKAKQAAQALGIELVKSNGQTKTNAELMDEAIKTYFGLGDAIDDTQNQA
                     DTLNGVWASTKATVGEALAPALTWVLNAVQWLTKAVKSLAVGFGGAVDLIVTQTMDMA
                     AVWGEVFNLKRLFTEGPSAYLSRISGAVDDWKRHFVTTLAGIGEQATEIWADTGEKSA
                     IVEEDARKKIIDQARLAAKAADDKEAAEAAKREAERARKAAELRLRSEIEVLERRLGL
                     ERKFSDNALAIEKQLIAKRAELERMGAEGNALALASIQEAERLDTEAKEKTYAAARKE
                     RALNLEIAMLEGQLAVTKNNTLERLQLELDLLAKKHEAAIAAAEASGDDTAVIDEKFR
                     AEWLERWLNGLERLGEHWRQTMAAINEWDLELLGENSREGLRIREQQIQDEKKRRIKA
                     GWDTAKAEQWATRETTQLKRQAMAAEAARIAELGNIAIGSLTTIFGEHKAFAIAQAII
                     NTWEGATKALSQGGIFGPALAAIVIAAGLKQVAEIRKTNPGGGGKSGKRGFDNPEHDR
                     LAFLAGEEGNRRWAGDMVRNWQLGAVAGWGKGLREAIASGVGNAATYDQRQTYNRGGD
                     VYNVYAYQAGDRQLRQLMRRARLVERRDQARRIG"
     gene            complement(3419..4042)
                     /locus_tag="PKK95_09210"
     CDS             complement(3419..4042)
                     /locus_tag="PKK95_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18434.1"
                     /translation="MRVGERRFVCRPPTLEALVRLLMLYPLEVQAIQGARLEHPELPA
                     TLDDLLPLFLADRARAATVLEACVALHGGVPGEARELLTADAGALEAIARAVVALCDV
                     EALARDLPPPHAKDSSPPELPADEQDSTWDIALCGVGRAFGVPPHELYAWPAEAVLAA
                     IRIVRLQELRGPAHEASAPRPAADIHASAEQLAVMGWPVQRAVKPEA"
     gene            complement(4078..4635)
                     /locus_tag="PKK95_09215"
     CDS             complement(4078..4635)
                     /locus_tag="PKK95_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18435.1"
                     /translation="MAVDPKKLAIGGCVVYVADWAANGGATGWTDVGLTEAGSELTDK
                     SENALIGDGDQVPCAQRAYPSTDEVEIKIVMKEADLAKLVWALQQPAANLTGTAPNQT
                     LLRGARTEQYKAIKLETAGNQGPTLVAATRTITAHRCYIKSRDGFQYGLKDKSQMYGV
                     TLGVLWDATVTTEDKLYKIVDSGGA"
     gene            complement(4668..4976)
                     /locus_tag="PKK95_09220"
     CDS             complement(4668..4976)
                     /locus_tag="PKK95_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18436.1"
                     /translation="MTKKVLLRFGGPNFPAGVGRVEFFQDLAQPLGPAGPYVEIRVDP
                     GDEFPLERVKDAQRFIDLGLAVLVEAVDGTLEETEGPPVPGEQLDAGDPPAELYQGRK
                     "
     gene            complement(4985..5950)
                     /locus_tag="PKK95_09225"
     CDS             complement(4985..5950)
                     /locus_tag="PKK95_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18437.1"
                     /translation="MAAAAEWAAWSLSGPAAIVDFLGYHSPTHAAQLKYEQSPGYPQP
                     DRYGAVSRIYDVTAGGLLTLTAWVRRYSGACGVAVQVQNPSGAGEWETVRAIAHDAPA
                     PDWTVLAVQVVAAGRLLGVRVITTLGFSDGTFLADDIAVTPDVRGGPMTLLQGYTALI
                     QRLKSLGGAGYWHDLRGENRVICNYFIPGQTGAPEPPYVCVPLFDDGDYPEGAQQGVT
                     EAELLIPITVWVAPADPPMLQSDVDLSCAAECLRWHDDLIKALWGTDGVRCWDLGLSR
                     VRVDLAAKSMRSEVTVEKDVQIGPHVRILVKLTTGFALAELGPSA"
     gene            complement(5950..6555)
                     /locus_tag="PKK95_09230"
     CDS             complement(5950..6555)
                     /locus_tag="PKK95_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18438.1"
                     /translation="MRLSGSIAIEGMGDLKKRLDNLGARKLKHTLIVGAVAGLQIVNV
                     EAARLAEAEEPEYMLWRTKAYSDTLRKRPRGRILSSSFVSTTVQAKVRLKTYGAAMET
                     GGTYRTRVRTHERRAPRPRRFAREPGLHGEPAGRELAMRTARITIRSYRRRQVQQARH
                     YLERGFERGRPKVQGVMERALAIALREDRVPRVAELMPGMA"
     gene            complement(6555..7214)
                     /locus_tag="PKK95_09235"
     CDS             complement(6555..7214)
                     /locus_tag="PKK95_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18439.1"
                     /translation="MPGRAIVDLGQLTGWLPATDGARNRQDDLTRCLDAACDRIERKC
                     CQRFLSNAYSAWHSGDRAVGKSRELLYLADPETGWATRRVTAITELTEDGAELPIILL
                     PDATTFADGEAAVVYAAQGKILRALVDAGKIVPKPWAYGVANIHVAYTAGYELADVPD
                     DLRQLAIELALLYFREGARQGQTSLNVEGVSLGYERLLRPEMRDVLRSYWQPVPQTAE
                     R"
     gene            complement(7219..7683)
                     /locus_tag="PKK95_09240"
     CDS             complement(7219..7683)
                     /locus_tag="PKK95_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18440.1"
                     /translation="MLRRWITFLVATAIALAPVQGAATRFALLASQAQTASANSSAVQ
                     VATITQAVVGIDITAGSGTVSDFDLWLEVSDDGGTSWYPLAPDVVIVDSTRAATWNVD
                     ADHAYIVDSKASTTAEKYLAVFKQVPADYVRLRWTLTGTTPSLTFSADMVGK"
     gene            complement(7731..8126)
                     /locus_tag="PKK95_09245"
     CDS             complement(7731..8126)
                     /locus_tag="PKK95_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18441.1"
                     /translation="MLEETKLVTSLQPQAFSGASAGDAVDTAGYDEAVVIFACGVIAA
                     NGTLDVKVQECDTSDGTFTDVEDAVFSQKTPTTDQAMYAGYIRCEGVRKRWLKAHATQ
                     ATAAATGAVAFLLGGKNFTGAPQSFAFNV"
     gene            complement(8126..>8398)
                     /locus_tag="PKK95_09250"
     CDS             complement(8126..>8398)
                     /locus_tag="PKK95_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18442.1"
                     /translation="KQDTPIVAPGVDPADQVGVVFHEGANIEHRKPGLVYVIDRALAH
                     HYVEVAKVAEYSDKAPPPPPREPNPPRPNDRAMQSPPRGRKGPEER"
BASE COUNT         1172 a         3028 c         2716 g         1482 t
ORIGIN      
        1 ccgggcacca gtccagcccg tacgcgtaga gctggtcgta ggtgttgagc gtcaccaccg
       61 tcgcgatcgc gacgccgttc ttccgcagct tcaggtcgct ccccacgagc gccagctcgt
      121 agtagtcgcc gcccgcgtaa tcccaggccc gcccgccggt gtcggacccg ctggccgcgc
      181 gcagcgtcgc cggctcggtc agcgcatacg ggcgccagtc gcacttcacg gtgtcggcgc
      241 cggcaccggt gaacccggac tgctggtcgg ccgcgtcgta gcggttgccg cccgcgtaga
      301 ccgcgatgtc cgagctgcgc tcgagcagtc ccgccggcga ccccgagggc aggcccgtgt
      361 cgttcatgtc ggtcggaaac ggcagctcgg cgagctgctg cgcgatgtcc acgtggacgt
      421 cggtgagccc ggcctcggcc gcgagacgcc cggccagggt ggtcacgctc tggctgcggt
      481 agtacggggt ctccagcgtc agcggcgccg ccgtgaacgc gttggtccac gtctcgagcg
      541 tcgtgacctg ggtcgagctg tccaccgtac ggatcgtctg cgtctcgctc tcgccaccgc
      601 cgtccagcgt gatctcgtcg ccggcggcca ggtccgtcgt gtcgctgacc gtcaccgtgc
      661 gactgccggc gcttaccgtg ccggtccggt cctcgactgt ccgccggacg gcctcggcgc
      721 tgtgcagctc gagcagcttc gagtacgcga acacctggag cgacacggtc cccgcgccct
      781 cggtctggtg aatcgagccg gggacgtcga gcacgccggc gaagacccgc ttccactgcg
      841 gctcggccag cccggtctcg cgctcgacga ccagctcgaa gacctggccc cgcaccgcgc
      901 cctcgagcag gccgctgacg accccgtcct cgtcgaagaa ctcgacgtcc atgtcggagt
      961 gcgtgagcag gccgaggtcc tcctccagct cctcggtgat ctgcccgaac gagtcgaggt
     1021 ccgtgcggtc ggtcaggtcg tgcaccaggc cgtccggcgt ggtcagcgtc acgcgaaact
     1081 ggcgcaggtc gctcatccaa tccgccgggc ctggtcgcgg cgctcgacca ggcgcgcccg
     1141 gcgcatcagc tgtcgcagct ggcgatcgcc ggcctggtac gcgtagacgt tgtagacgtc
     1201 gccgccgcgg ttgtaggtct gacgctgatc gtacgtcgcc gcgttcccga cgccgctggc
     1261 aatcgcctca cgcagtccct tgccccaacc agcgaccgcg ccgagttgcc agttgcgcac
     1321 catgtcgccc gcccagcgtc ggttcccttc ttcgccggcg agaaacgcca ggcgatcgtg
     1381 ctccgggttg tcgaagccgc gcttgccgct cttgcccccg ccgcctgggt tcgtcttccg
     1441 aatctcggcg acttgtttca gacctgccgc gatcacgatc gcagccagcg ccggtccgaa
     1501 gatgccgccc tgagaaagcg ctttggtcgc accctcccac gtgttgatga tcgcctgggc
     1561 gattgcgaac gccttgtgct cgccgaaaat cgtagtgagc gacccgatcg cgatgttgcc
     1621 gagttcggcg atccgcgcgg cctcggcagc catggcctga cgcttcagct gcgtcgtctc
     1681 tctggttgcc cactgctcgg ctttcgccgt atcccacccg gccttgatcc gccgcttctt
     1741 ctcgtcctgg atctgctgct cgcgaatcct caggccctcg cggctgttct cgccgagcag
     1801 ctccagatcc cattcgttga tcgcggccat ggtctgccgc caatgttccc cgagccgttc
     1861 aagcccgttc agccatcgct cgagccattc ggctcgaaat ttttcgtcga taaccgcggt
     1921 gtcgtcgccc gatgcttcgg ccgcagcgat cgccgcttcg tgcttcttcg ccagcagatc
     1981 cagttcgagc tgtagccgct caagcgtgtt gttcttcgtt accgcaagct gtccctcgag
     2041 catcgcgatt tcgaggttga gcgcccgctc ctttcgggcg gccgcgtacg tcttctcctt
     2101 ggcctcggtg tcgaggcgct cagcctcctg aatagatgcc agggcgagtg cgttgccctc
     2161 ggcgcccatg cgctccagtt ccgcgcgctt ggcgatcagc tgcttttcga tcgcgagtgc
     2221 gttgtcgctg aacttgcgtt cgagccccag ccggcgctcc agaacctcga tttccgagcg
     2281 caaccgcagt tcagccgcct tgcgcgcgcg ctcggcttca cgcttcgccg cctcggctgc
     2341 ctccttgtcg tccgcggctt tggcagccag tcgcgcctga tcgatgatct tctttcgcgc
     2401 gtcttcctcg acgatggcgc ttttctcacc ggtgtcggcc cagatctccg tcgcctgctc
     2461 accaatgccg gcgagcgttg tgacgaagtg gcgcttccag tcatcgaccg cgccggagat
     2521 gcgcgagaga taagcgctcg gcccctccgt gaacaggcgc ttcaggttga agacctcgcc
     2581 ccagacggcg gccatgtcca tggtctgtgt gacgatcaga tcgacggcgc ccccgaagcc
     2641 gaccgcgagc gacttcaccg ccttcgtgag ccactgaacg gcgttgagga cccaggtcag
     2701 cgccggcgcg agcgcttcgc cgacggtggc cttggtgctg gcccagacgc cattgagcgt
     2761 gtccgcctgg ttctgcgtgt cgtcgatcgc gtcgccgagg ccgaagtagg tcttgatcgc
     2821 ctcgtccatc agctctgcgt tggtctttgt ctggccattc gacttgacca gctcgatgcc
     2881 gagcgcctgg gcggcctgct tggccttgcc ctgcatgatc gccgccagcg cggccccggc
     2941 cgccccggcc tccatgaatc cggcctcgga gatgtcggcc gcgagcttcg tcgcgtacag
     3001 cgcggcatcg gtgtccttcg tgatcccgat gaacttctgg aagatcggga tcgtctcgga
     3061 aacgagcgga ccgccgctgt cctggatcgc ctgcaggccg gccttgatct cgggcaggct
     3121 gcccttcgcg tccaggccca ggcgctgcat ctggaagccg atggcgttga agccccgctc
     3181 cagcttcgcg aattcggtga tgctgctctt cacgaacgcg gcgatggtgc caagagcgaa
     3241 cgcgccggcg aggtactggc cgacctgttt cccagcgtcg cccaagccgc ccatcttctt
     3301 cttcgtctcg tcgagcttct tgtccagctt gtcgagacgg gatttcagct ccagaacacc
     3361 gttgtcctgg agcaccatct tcacgatccc ggcgagcgtg acgctcttct cggccatgtc
     3421 aggcctccgg tttcaccgcg cgctgcaccg gccagcccat caccgcgagc tgctcggccg
     3481 aggcgtggat gtccgcggcc ggcctgggtg ccgatgcctc gtgggccggc ccgcgaagct
     3541 cctgcaggcg aacgatccgg atcgccgcca ggaccgcttc cgctggccag gcgtacaact
     3601 cgtgaggcgg aacgccgaac gcgcggccga ccccgcagag cgcgatgtcc cacgtcgaat
     3661 cctgctcgtc ggcgggcagc tcgggcggtg acgagtcctt tgcgtgcggc ggcggtaggt
     3721 cgcgcgccag agcctcgacg tcacagagcg ccaccaccgc gcgcgcgatc gcctcgagcg
     3781 cgcctgcgtc tgccgtcagc agctcacgcg cctcccccgg aacgccaccg tgtagtgcga
     3841 cgcaggcctc cagcaccgtg gccgcgcgtg cacgatccgc gaggaacagc ggcagcaggt
     3901 cgtcgagcgt ggccggcaat tccgggtgct cgagccgggc cccctggatt gcctgaactt
     3961 cgagcgggta cagcatcagc aatcgaacca gcgcctcgag cgtgggcggg cggcacacga
     4021 agcgccgctc gcccactctc actacgcgct caacgcgccg gaaggggctg ccgtacatca
     4081 cgcgccgccg ctgtcgacga tcttgtacag cttgtcctcc gtggtcaccg tggcgtccca
     4141 gagcaccccg agggtcacgc cgtacatctg cgacttgtcc ttcagaccgt actggaagcc
     4201 atcgcgcgac ttgatgtagc agcggtgcgc cgtgatcgtc cgcgtggcgg ccaccagcgt
     4261 cgggccctgg ttgccggcgg tctccagctt gatcgccttg tactgctccg tgcgggcccc
     4321 gcgcagcagc gtctggttcg gcgccgtgcc ggtcaggttc gcggccggct gctgcagcgc
     4381 ccacaccagc ttcgcgaggt cggcttcctt catcacgatc ttgatctcga cctcgtccgt
     4441 ggaagggtac gcacgctgcg cacacggcac ctggtccccg tcgccgatca gggcgttctc
     4501 gctcttgtcg gtcagctcgg acccggcctc ggtcagaccg acgtcggtcc acccggtggc
     4561 cccgccgttc gcagcccagt cggcgacgta cacgacgcag ccgccgatgg ccagtttttt
     4621 cggatccacg gccatgcatc acctcctgcg gcaggcgccg cggtgcatta cttccgtccc
     4681 tggtagagct ccgccggcgg atccccggcg tcgagctgct cgcccgggac cggtggcccc
     4741 tccgtttcct ccagcgttcc atcgacggct tcaaccagga cggccaagcc gagatcgatg
     4801 aagcgctgcg catccttgac acgctccagc gggaactcgt ccccggggtc cacgcgaatc
     4861 tcgacgtagg gtcctgcggg gccgagcggc tgcgcgaggt cctggaagaa ctcgacccgc
     4921 ccaacgccgg ccgggaagtt gggaccgccg aagcgcagca gcactttctt cgtcatgaga
     4981 gtcctcacgc gctcggcccg agttcggcca gcgcgaagcc cgtcgtgagc ttgaccagga
     5041 tccggacatg cggtccgatc tggacgtctt tctcgaccgt gacctccgag cgcatcgact
     5101 ttgctgccag gtcgaccctc acgcgcgaga gaccgagatc ccagcaccgc acgccgtcgg
     5161 tgccccacag cgccttgatc aggtcgtcgt gccatcgcag gcattccgcc gcgcacgaca
     5221 ggtcgacatc cgactgcaac atcggaggat ccgcgggggc gacccagacc gtgatcggga
     5281 tcagcagctc ggcctcggta acgccctgct gcgcgccctc cggataatcg ccgtcatcga
     5341 acagcggtac gcaaacgtac ggaggctccg gcgcgcctgt ctgcccgggg atgaagtagt
     5401 tgcagatcac ccggttctcg cctctcagat cgtgccagta gccggcacca ccgagcgact
     5461 tgaggcgctg gatgagtgcg gtgtagccct gcagcagcgt catcggacct ccccttacgt
     5521 ccggggtaac cgcgatgtcg tcagccagga acgtcccgtc agagaaaccg agagtggtga
     5581 tcacgcgtac gccgagcagc ctcccggcgg cgacgacctg gacggccagg accgtccagt
     5641 ccggagccgg cgcgtcgtgc gcaatcgccc ggacggtctc ccactcgccg gcaccggacg
     5701 ggttctgcac ttggacggca acgccgcaag cgccgctgta gcgccgcacc caggcggtga
     5761 gcgtcaaaag cccgccggcc gtcacgtcgt agatgcgcga gacggcgccg taacggtccg
     5821 gctgcggata gcccgggctt tgctcgtact tgagctgcgc ggcatgcgtg gggctgtgat
     5881 agcccaggaa gtccacaatc gcggccggcc cactcaggct ccacgccgcc cactcggccg
     5941 ccgcggccat caggccatcc ccggcatcag ctcggccacg cgcgggacgc gatcctcgcg
     6001 gagcgcgatc gccagcgcgc gctccatcac accttgcact ttcggcctcc cgcgctcgaa
     6061 accgcgctcc aggtagtgcc gcgcctgctg cacctggcgg cgccgatagc tgcggatcgt
     6121 gatccgagct gtgcgcatgg ccaactcgcg cccggccggc tcgccgtgca gccccggctc
     6181 acgcgcgaac cgccttggcc gcggcgcccg acgctcgtgg gtgcgcaccc gcgtgcggta
     6241 ggtcccgccg gtttccatcg cggcgccgta cgtcttgagc cggaccttcg cctgcacggt
     6301 cgtgctcacg aacgagctgg agaggatccg cccgcgcggt cgcttccgca gcgtgtcgga
     6361 gtaggccttg gtccgccaga gcatgtactc gggctcctcg gcctcggcca gccgcgcggc
     6421 ctcgacgttc acgatctgta ggccggcgac ggcgccgacg atgagcgtgt gtttcagctt
     6481 gcgcgcgccg aggttgtcca ggcgcttctt caggtcgccc ataccctcga tcgcgatgga
     6541 gcccgagagc cgcatcaccg ttccgccgtc tgtggaacgg gctgccaata cgaccgcaac
     6601 acatccctca tttccggccg cagcagacgc tcgtacccca ggctgacacc ttcgacattg
     6661 agactggtct ggccctggcg cgccccctcg cggaagtaga gcagcgccag ctcgatcgcc
     6721 agctggcgga ggtcgtccgg aacatcggcg agttcgtagc cggccgtgta cgcgacgtgg
     6781 atgttcgcga cgccgtacgc ccagggcttc ggcacgatct tcccggcatc gaccagcgcg
     6841 cgcaggatct tcccctgcgc ggcgtagacc accgcagcct cgccgtcggc gaaggtcgtc
     6901 gcatccggca ggaggatgat cgggagttct gcgccgtcct cggtcagctc cgtgatcgcc
     6961 gtcacccgcc gcgtggccca gcccgtctcg ggatcggcca ggtacagcag ctcgcggctc
     7021 ttgccgacgg cgcgatcacc gctgtgccag gccgagtacg cgttggacaa gaagcgctgg
     7081 cagcacttgc gctcgattcg gtcgcaggcg gcgtcgaggc agcgcgtgag atcgtcctgc
     7141 cgattgcggg cgccgtccgt cgccggcaac caaccggtga gctggcccaa gtccacgatc
     7201 gcgcggccgg gcatcgcgtt acttcccaac catgtccgcg ctgaacgtca gcgatggcgt
     7261 tgtcccggtc agcgtccatc gcagtcgcac gtagtcggcc ggcacctgct tgaagaccgc
     7321 gaggtacttc tcagccgtcg tcgacgcttt cgagtccacg atgtacgcgt ggtcggcatc
     7381 gacgttccac gtggccgccc gtgtcgagtc cacgataacg acgtcgggtg cgagcgggta
     7441 ccagctggtt ccgccgtcgt ccgagacctc gagccacagg tcgaagtcgc tgacggtccc
     7501 tgagcccgcg gtgatgtcga tcccgacgac cgcctgcgtg atcgtggcga cctggacggc
     7561 cgagctgttg gcgctggccg tctgcgcctg ggaggcgagc agcgcgaacc tcgtcgcggc
     7621 gccctggaca ggggccagcg cgatcgccgt cgcgaccaag aaagtgatcc agcgtcggag
     7681 catcggtcct ccccgcgggg ccggcgccgc gacgccgacc ccccgactga tcacacgttg
     7741 aaggcgaacg actggggcgc gccggtgaag ttcttgccgc cgagcaggaa cgcgaccgcg
     7801 cctgtcgccg ccgcggtggc ctgcgtcgcg tgcgccttca gccagcgctt gcgcacgccc
     7861 tcgcagcgga tgtacccggc atacatcgcc tggtcggtgg tcggggtctt ctggctgaac
     7921 accgcgtcct cgacgtcggt gaacgtcccg tctgacgtgt cgcactcctg gaccttgacg
     7981 tcgagcgtgc cgttggccgc gatcacgccg caggcgaaga tcacaaccgc ttcgtcgtag
     8041 ccggccgtgt cgacggcatc gccggcgctg gcgccggaga acgcctgcgg ctgcagggac
     8101 gtgacgagtt tggtttcttc gagcatcagc gttcctccgg tcccttccga ccgcgggggg
     8161 gagactgcat ggcgcggtcg ttcggccgcg gcgggttggg ctcgcgcggc ggtggcggcg
     8221 gcgccttgtc cgagtactcg gccactttcg cgacctccac gtaatggtgg gcgagcgcgc
     8281 gatcgatgac gtagacgagc cccggcttgc ggtgctcgat gttcgcgccc tcgtggaaca
     8341 cgacgccgac ctggtccgcc gggtccactc ccggcgcgac gatcggcgtg tcctgttt
//
LOCUS       DAOPQX010000149         8394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9471_length_8394_cov_4.851061, whole genome shotgun sequence.
ACCESSION   DAOPQX010000149 DAOPQX010000000
VERSION     DAOPQX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9471_length_8394_cov_4.851061"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..888)
                     /locus_tag="PKK95_09255"
     CDS             complement(112..888)
                     /locus_tag="PKK95_09255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC18443.1"
                     /translation="MPNRPRSTANDEVRQYWNTRIHDLEISKHPPGSPGFFADLDEYH
                     FDKLHHLLRLVNFDGYRGRRVLDVGCGAGVDLVRFLRGGALGTGVDLAPAAIDLAWTN
                     LEQQGLQAELLVADGESLPFPDGRFDLVFAHGVVQYTGDDRRLVEECRRVLKPGGLAI
                     FQVYNRISWLNLLSRVMKVGLEHEDAPVLRKYSPAEFRRLLAGFADVRVVPERFPVKS
                     RLHGGWKGTLFNTFFVGTFNALPRAWVRRFGWHLLAFCTR"
     gene            940..2556
                     /locus_tag="PKK95_09260"
     CDS             940..2556
                     /locus_tag="PKK95_09260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOC18444.1"
                     /translation="MQRDLSRLARETFDVLVVGGGIHGLAAAYDAAQRGFSVALVERG
                     DFGSGASFNHQKTIHGGLRYLQSGDLKRMRESILERRTLARIAPHLLTPLPFLMPTYL
                     KLTRSRLAMWVAFRTDALVGSDRNDGVTPRLHLPEGRLLSKAECLEAFPDVRQKGLTG
                     GALWHDYQVRNTDRLNLAFALAADAAGACLANHVEAIAPALEKGRAVGARVRDALTAC
                     EFDVRARMTLNAAGAATGRLMASLGRAEAHPLIKAMNLVTRRPFSGPALASSTDEGRM
                     LFIVPWEGRAVAGTSHSPEPCGPEETAVSERELTWFIGQVNEAFPSLRLTVEDVTLVH
                     RGIVPAARSRKGEWGLEGHFRLRDHAADGVEGAVSLVAVKYTTGRGVAEAAVDLVARK
                     LGRRVPSRTAQVPLPGGEVADLETLEEMALRDAAGLVDNEVAAQLALTYGASYPALLQ
                     LVRESPPLAARLAPAVPVIAAQVVHAVRDEMTFTLTDLVTRRVPLGSMGHPGATAASA
                     CATLMAKELGWDAARTERELQELRDFYRPV"
     gene            complement(2541..2768)
                     /locus_tag="PKK95_09265"
     CDS             complement(2541..2768)
                     /locus_tag="PKK95_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18445.1"
                     /translation="MNEIVLVVQEVPEVLKVLVPRVLVLKVLVPRVLVPSVLVPKVLV
                     PSVLVPKVLVPKVLVPKVLGACPREQPQTGR"
     gene            complement(2770..3633)
                     /locus_tag="PKK95_09270"
     CDS             complement(2770..3633)
                     /locus_tag="PKK95_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18446.1"
                     /translation="MLLRTRLAPFKLIAVLLFVVVTSAPVFAQQRPLQTQDPEAIGAG
                     NVLIEAGVDYGRSIFFPASGLEGHLWRAPALGVVIGVGSIAEIQLTGSPYKRLTITER
                     NDAPLADLVEPGDTTSTVEDLVIGTKLRLLAESETTPAIAVRIATRLPNASESSGIAL
                     DTTEFYASILAGKTIESVRVALNAGLGILPDPILASRQNDVLTYGLSLARALDERLDV
                     VGELNGRINTRGDEAPLGTESLSQFRAGLRYTIGSARLDLGGIVGLTSRDPGIGFTAG
                     FTYMFKAFDVK"
     gene            complement(3666..4346)
                     /locus_tag="PKK95_09275"
     CDS             complement(3666..4346)
                     /locus_tag="PKK95_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC18447.1"
                     /translation="MRRVLLVEDEPGLVLTLTDRLKSEGFEVASAQDGRSGLARAAGE
                     AWDVILLDVMLPGMSGFDLCRDLRQKGVRTPIIMLTARGQVIDKVLGLKLGADDYLTK
                     PFDMLELLARIEVQLRRAAPGPSGAHEFRFGDIVVDTRKAEVWRTGAPLDLSAREFLL
                     LKYFLEHRGDTLTREQLLNEVWGYNSVPSTRTVDVHVAWLRQKIEPNPRHPQYILTVH
                     GLGYKFAG"
     gene            complement(4343..6283)
                     /locus_tag="PKK95_09280"
     CDS             complement(4343..6283)
                     /locus_tag="PKK95_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOC18448.1"
                     /translation="MVRLRRLPLGVLVPAALVVLVATLAGLQYRWLGKVSEAEREQLR
                     RSLDQRARELVDEFDGEITRAYLALHLSEAEMAAGDWERFAVAVERWRNAARFPGMVR
                     DIVLAQSKQGEWIFRRYDPQVRAFRPPIAGWPPRLEGLRSYLAGEKSTGKVEREGRPV
                     EFRLTLPPVAPALPALLVPVGASLPLLPRRPSLPEPRSGPGEDAARQVFEARFWLRAP
                     VAFVVADLDQDVLRTSVLPALVERHLPEGADSYRVAVLSASGQPLFARGLEPGASLDP
                     EQADSVSPFFNLRLDVLRGIDAGAGQLTLRTRVAGADPAPEGADAAGGPRMAIVVERR
                     DDVGPIPHATGAVRGLQSWRLVLQHPAGSLDAAVAQARRRNLWLGGGILAVLLAGVAL
                     VVRNGRRASQLAARQMEFVTTVSHELRTPLAVIRSAAQNLSAGVVSEPAQARRYGELI
                     EDEGRRLTEMVEQILEYSRVHGDGPVRDPQPLDVASLLADLRASCEALCAQAGIALEV
                     ECPPEGTPPILGDEPAVRRALHNLVGNAVKHAAEGKWIGVTASVGAVRGRPQVAIAVR
                     DRGPGLVPADLAHVFEPFYRGRQAIERQVHGNGLGLSLVKRVAEAHGGSVAVWSEPAK
                     GSTFTLTLPAVPEAAAPAGERP"
     gene            6516..7505
                     /locus_tag="PKK95_09285"
     CDS             6516..7505
                     /locus_tag="PKK95_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18449.1"
                     /translation="MKRSLTALFVLGSFALLSAQGKDAAEVLADARQALGGTQLESLK
                     SLSGKGRTLRSTPRGDTVETEFEVAFELPDKYRLRTALMPMGNMTIYREIGFNAGRLI
                     EQVDRPPNLSGGMVHIRVAGPGGPVDPEKMTPEQKAEFERQRVLANKKEYARLALGLF
                     AASPGFPLEFAYAGEAEAPDGKADVIDVKGPGDFAARLFVDQATHLPLMLSWMDKEPL
                     TIRMGPGGPSWTAGGGGGGTVVQRLEQRSGPMSKEEREKLEKEMEEARAKAEADRRTV
                     EFRVYYADYREVGGVRLPHRIQRSIDGKAAEEMEFDSLKVNAKIDARTFEPTK"
     gene            7669..>8394
                     /locus_tag="PKK95_09290"
     CDS             7669..>8394
                     /locus_tag="PKK95_09290"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOC18450.1"
                     /translation="MLKVPLVLCAALIALAPSPLLAGEQASSARVVVTVLDQTGAVIL
                     QAPVSLTPEDGGAEAAVPREALSNGLGVAAFDNLPEGRYTVRAQFPGFQQATVTGVRA
                     RRGREARVRVVLQLEKLDENLTVGRDRQTSALDPRGSAFSSVLTREQIDALPDDPDEM
                     EAVLKALAPPGSVIRVDGFSGGRLPPKSQIRSIRLPRLDTFAAQNHGGMMGAHFIDIM
                     TMPGNGPLRGNVDFNFQDEALNAR"
BASE COUNT         1286 a         2961 c         2947 g         1200 t
ORIGIN      
        1 gtgcgcgcca acgcggcggg gtcctgcccc gcggcatcgt cctcggtcac gtacaggaaa
       61 tcgttgccgt aggcgtgcgc cttgaggaaa ggcagcatgg acggttctcc ctcaccgggt
      121 gcagaacgcg aggaggtgcc agccgaagcg cctgacccag gcgcgcggga gcgcgttgaa
      181 ggtgccgacg aagaacgtgt tgaacagggt gcccttccag ccgccgtgca ggcgcgactt
      241 caccgggaac cgctcgggaa ccacccgcac gtcggcaaag cccgccagca ggcgccggaa
      301 ctcggccggg ctgtacttcc ggaggaccgg ggcgtcctcg tgctcgaggc cgaccttcat
      361 cacgcgcgag agcaggttca gccacgaaat ccggttgtag acctggaaga tggcgaggcc
      421 tccgggcttc agcacgcgcc ggcattcctc gacgagccgg cggtcgtcgc ccgtgtactg
      481 cacgacgccg tgggcgaaca cgaggtcgaa gcggccatcc gggaacggca ggctctcgcc
      541 gtcggccacc aacagctcgg cctgcagccc ctgctgctcg aggttcgtcc aggccaggtc
      601 gatcgccgcc ggcgcgagat cgacgccggt gcccagcgcc ccgccccgga ggaagcggac
      661 gaggtccacc ccggcgccgc agccgacgtc gagcacgcgc cgcccgcgat agccgtcgaa
      721 gttcacgagc cgcagcaggt ggtgcagctt gtcgaagtgg tactcgtcga ggtcggcgaa
      781 gaagccgggc gacccgggcg ggtgcttgct gatctcgaga tcgtggatcc gcgtgttcca
      841 gtactggcgg acctcgtcgt tggcggtcga tcgcggtcgg ttgggcacgg ccgcgtacta
      901 tagaataggc ggctgcgctg gagcaacgac ggggaccctg tgcaacgcga cctctctcgc
      961 ctcgctcgtg agaccttcga cgtcctggtc gtcgggggcg gcatccacgg cctcgccgcg
     1021 gcctacgacg ccgcccagcg cggcttctcg gtggcgctgg tcgagcgcgg cgatttcggg
     1081 tcgggcgcct cgttcaacca tcagaagacg atccacggcg gtcttcgcta cctgcagtcg
     1141 ggcgacttga agcggatgcg cgagtcgatc ctcgagcgcc gcacgctcgc ccggatcgcc
     1201 ccccacctgc tcacgccgct gccgttcctg atgcccacct acctcaagct gacgcgcagc
     1261 cggctggcga tgtgggtggc gttccggacg gacgccttgg tgggcagcga tcggaacgac
     1321 ggcgtcacgc cgcggctcca cctgccggag ggccgcctgc tctcgaaggc cgagtgcctc
     1381 gaggcgtttc ccgacgtccg gcagaagggg ctgaccggcg gcgccctgtg gcacgactac
     1441 caggtccgca acaccgaccg cctcaatctc gccttcgcgc tggcggccga cgcggccggc
     1501 gcctgcctgg ccaaccacgt cgaggcgatc gcccccgcgc tcgagaaggg gcgggccgtt
     1561 ggcgcccgcg tccgtgacgc gttgaccgcc tgcgagttcg acgtgcgggc gcgcatgacg
     1621 ctcaacgccg ccggggccgc cacgggacgg ctgatggcct cgctcggccg cgccgaggcg
     1681 cacccgctca tcaaggcgat gaacctcgtg acccggcgcc ccttctcggg gccggcgctc
     1741 gcctcctcga ccgacgaggg ccggatgctg ttcatcgtgc cgtgggaagg gcgggcggtc
     1801 gccggcacgt cgcactcgcc cgaaccgtgc ggccccgagg agaccgcggt ctccgagcgc
     1861 gagctcacgt ggttcatcgg ccaggtgaac gaggcgttcc cctcgctgcg gctcacggtg
     1921 gaggacgtca cgctggttca ccgcggcatc gtgccggccg cccggagcag gaaaggcgaa
     1981 tggggcctcg aggggcactt ccggcttcgc gaccacgcgg ccgacggcgt ggaaggagcg
     2041 gtgtcgttgg tggcggtgaa gtacacgacc ggccggggcg tggccgaagc ggccgtggat
     2101 ctcgtggccc gcaagctcgg acgccgcgtc ccctcgcgca ccgcccaggt cccgctgccg
     2161 ggaggcgagg tggccgacct cgagacgctc gaggagatgg cgctccgcga cgcggccgga
     2221 ctggtcgaca acgaggtggc ggcgcagctg gccctgacct acggggcgag ctaccctgcc
     2281 ctgcttcagc tcgtccgcga gtcgccgccg ctggcagcgc gcctggcgcc cgccgtgcca
     2341 gtcatcgccg cgcaggtggt gcacgccgtg cgcgacgaga tgaccttcac gctcaccgac
     2401 ctcgtgacca ggcgtgttcc gctcggctcg atgggccacc ctggcgcgac cgctgccagc
     2461 gcctgtgcaa ccctgatggc gaaggaactc ggctgggacg cggcgcggac ggagcgggag
     2521 ctgcaggaac tcagggattt ctatcgcccg gtctgaggct gctcccgcgg gcaggccccc
     2581 agcaccttcg gcaccagcac cttcggcacc agcaccttcg gcaccagcac gctcggcacc
     2641 agcaccttcg gcaccagcac gctcggcacc agcaccctcg gcaccagcac cttcagcacc
     2701 agcaccctcg gcaccagcac cttcagcacc tccggcacct cctgcaccac cagcacgatc
     2761 tcgttcactt cacttcacgt caaacgcctt gaacatgtag gtgaaccccg cggtgaaccc
     2821 gatgccgggg tcgcgcgagg tcaaacccac gatgccgccg aggtcgaggc gggccgagcc
     2881 gatcgtgtaa cgcaggccgg cgcgaaactg gctgagcgac tcggtcccga gcggcgcctc
     2941 gtcgccgcgc gtgttgatcc ggccgttcag ctcgccgacc acgtccagcc gctcgtcgag
     3001 cgcgcgcgcg agcgacaggc cgtaagtgag cacgtcgttc tggcggctgg ccaggatcgg
     3061 gtcgggcagg atccccaggc ccgcgttcag cgcgacgcga accgactcga tggtcttccc
     3121 cgccagaatc gacgcgtaga actcggtcgt atcgagcgcg atgccgctcg actcgctcgc
     3181 gttcggcagg cgcgtcgcga tccgaacggc gatggccggc gtcgtctcgc tctcggccag
     3241 caggcgcagc ttggttccga tcaccaggtc ctcgaccgtg ctggtcgtgt cgcccggctc
     3301 caccaggtcg gcgagcggcg cgtcgttgcg ctcggtgatg gtcaggcgct tgtacggcga
     3361 gccggtcagc tggatctcgg cgatcgaccc cacgccgatc acgacgccga gcgccggcgc
     3421 gcgccacagg tggccttcga ggcccgacgc cgggaagaag atgctgcgac catagtcgac
     3481 gccggcctcg atgaggacgt tccctgcccc gatcgcctcg ggatcctggg tctggagtgg
     3541 acgctgctgc gcgaaaacgg gggccgaagt gacgacgacg aagaggagga cggcaatcaa
     3601 cttgaatggg gcgagccgcg tgcggagaag catgccctat tgtacaaccc gctcgcggcc
     3661 tcgattcacc ccgcgaactt gtacccgaga ccgtgcaccg tgaggatgta ttgcgggtgg
     3721 cgcgggttgg gttcgatctt ctggcgcagc caggcgacgt ggacgtccac cgtccgcgtg
     3781 gacggcaccg agttgtagcc ccagacctcg ttcagcagct gctcgcgcgt gagcgtgtca
     3841 ccgcggtgct cgagaaagta cttgaggagc aggaactcgc gcgccgagag atcgagcggc
     3901 gccccggtgc gccacacctc ggccttccgg gtgtccacga cgatgtcgcc aaagcggaac
     3961 tcgtgcgcgc cggaagggcc gggggcggcg cggcgcagct gcacctcgat gcgtgcgagc
     4021 agttcgagca tgtcgaaggg tttcgtcagg tagtcgtcgg cccccagctt caggccgagc
     4081 accttgtcga tcacctggcc gcgggcggtc agcatgatga tgggggtgcg aacgcccttc
     4141 tgccgaaggt cgcggcacag gtcgaagccg ctcatcccgg gcagcatcac gtccagcagg
     4201 atcacgtccc acgcctcgcc ggccgctcgc gcgaggccag accggccgtc ctgggcggaa
     4261 gcgacctcga agccttcgct cttgaggcga tcggtgaggg tcagcaccag gccgggctcg
     4321 tcctcgacga gaaggacacg cctcacggcc gttccccggc aggcgcggcg gcttctggca
     4381 cggcgggcag cgtcagcgtg aaggtgctgc ccttcgccgg ctccgaccac accgcgacgg
     4441 agccgccgtg ggcttccgcg acgcgcttca cgaggctgag gccgagaccg ttgccgtgaa
     4501 cctgccgctc gatcgcctgc cgtccccggt agaacggttc gaagacgtgc gccaggtcgg
     4561 cggggaccag gcccggtccc cggtcgcgca ccgcaatcgc cacctgggga cggccgcgaa
     4621 cggcgccaac gctggccgtg acgccgatcc acttcccctc ggccgcgtgc ttcacggcgt
     4681 tgcccaccag gttgtgcagg gcccgccgca cggccggctc gtccccgagg atcgggggcg
     4741 tgccctccgg cggacactcc acctcgaggg cgatgcccgc ctgcgcacac agcgcctcgc
     4801 acgaggcccg gaggtcggcg agcagcgagg cgacgtcgag cggttgcggg tcgcggactg
     4861 gtccgtcgcc gtgcacgcgg gagtactcga ggatctgctc gaccatctcg gtcaggcggc
     4921 ggccctcgtc ctcgatcagc tcgccgtagc gcctggcctg cgccggctca gagacgaccc
     4981 cggcggagag gttctgcgcg gcggaccgga tcaccgcgag cggcgtacgc agctcgtgcg
     5041 agacggtcgt cacgaactcc atctgccgtg ccgcgagctg actcgcgcgc cgcccgttgc
     5101 ggacgacgag cgcgacgccg gcgagcagca cggccaggat gccgcccccg agccagaggt
     5161 tccggcggcg ggcctgcgcg acggcggcat cgagcgatcc ggccggatgc tggaggacga
     5221 ggcgccacga ctgaagccct cgcaccgcgc cggtggcgtg cgggattggc cccacatcgt
     5281 cccggcgctc cacgacgatg gccatgcgcg ggcccccggc ggcgtccgca ccctcggggg
     5341 ccgggtcggc gccggccacc cgtgtgcgca gcgtcagctg gccggcaccg gcgtcgatcc
     5401 cgcgcagtac gtccagccgg agattgaaga aggggctgac gctgtctgcc tgctccgggt
     5461 cgagcgaggc tcccggttcc agaccccgtg cgaagagcgg ctgtccggaa gcggagagaa
     5521 cggcgaccct gtagctgtca gctccctcgg gcaggtgccg ctcgacaagg gccggaagca
     5581 cgctggtccg aagcacgtcc tggtcgaggt cggccacgac gaaggcgacc ggcgcgcgaa
     5641 gccagaagcg agcctcgaac acctggcgcg cggcgtcctc ccccggtccg gatcgcggtt
     5701 ccggcaacga aggccggcgc ggaagcagcg gcagcgacgc gccgaccgga acgaggagtg
     5761 ccggcagggc cggtgcgacc gggggcaggg tgaggcggaa ctcaaccgga cgtccctcgc
     5821 gctcaacctt accggtcgac ttctccccag ccaggtagct gcgaagcccc tccaggcgcg
     5881 gaggccatcc tgcgatcggg gggcggaagg cgcgaacctg cgggtcgtag cgcctgaaga
     5941 tccactcgcc ctgtttcgac tgcgcaagga cgatatcccg gaccatcccc ggaaagcgcg
     6001 ccgcgttgcg ccacctctcc accgccacgg cgaagcgttc ccagtcgccc gcggccatct
     6061 cggcctcgga caggtgcagg gcgaggtaag cgcgggtgat ctcgccgtcg aactcgtcca
     6121 ccaactcgcg cgcccgctgg tcgagcgagc gccgcagctg ctcgcgctcg gcctccgaca
     6181 ccttgccgag ccagcggtac tgcagccccg caagcgtcgc caccaggacg acgagcgctg
     6241 ccggcacgag cacgccgagc gggagccgcc gaagcctcac cacgacgcga gtgtactatc
     6301 agccctcttc cccggggccc tcgcttccag ccgctcggcc cggtttttac cttcttaacg
     6361 atctctttgc cccggcttaa ccattcgggt ccgtccaacc ggcatcatcg gttcgtcata
     6421 tgtgctgaca gcggacagac agtcgctgga gggcgaccac aagggtcgcc cctacgctcc
     6481 ggtggccgct acccggttcc atggaggcac caaccgtgaa acgttccttg accgccctgt
     6541 tcgttctcgg ctccttcgcc ctgctctccg cgcagggcaa ggatgccgcg gaggtgctcg
     6601 ccgacgcgcg tcaagcgctc ggcggcacgc agctcgaatc gctgaagtcc ctctcgggca
     6661 aggggcgcac gctgcgctcc acgccgcgag gcgacaccgt cgagaccgag ttcgaggtgg
     6721 cgttcgagct gcccgacaag taccgcctcc gtacggcgct gatgccgatg ggcaacatga
     6781 ccatctaccg cgaaatcggc ttcaacgccg gccggctgat cgagcaggtg gaccgtccgc
     6841 cgaacctctc gggcgggatg gtgcacatcc gggtcgcggg gccgggcggc ccggtcgatc
     6901 ccgagaagat gacgcccgag cagaaggccg agttcgagcg gcagcgggtg ctggcgaaca
     6961 agaaggagta cgcgcggctt gccctggggc tctttgccgc ctcgccgggc ttcccgctgg
     7021 agttcgccta cgccggcgag gccgaggcgc ccgacggcaa ggcggatgtc atcgacgtca
     7081 aggggccggg cgacttcgcg gcccgcctct tcgtggacca ggcaacgcac ctgcccctca
     7141 tgctgagctg gatggacaag gagccgctga cgatccggat gggcccgggt ggcccgagct
     7201 ggaccgcagg gggtggcggc ggcgggaccg tggtgcagcg gctcgagcag cgctcggggc
     7261 cgatgtcgaa agaggagcgc gagaagctcg agaaggagat ggaagaggcg cgtgcgaagg
     7321 cagaggccga ccgccgcacc gtcgagttcc gcgtctacta cgccgactac cgcgaggtgg
     7381 ggggcgtgcg gctgccccac cgcatccagc ggtcgatcga cggcaaggcg gccgaggaga
     7441 tggagttcga ctcgctgaag gtgaacgcga agatcgatgc ccgcaccttc gagccaacga
     7501 agtagcgagg gaggtgacgc aggcgtcccg cttctcgagg gtagtgggac aggcgtctcg
     7561 cttttcgacg tagtgggaca ggcgtctcgc ttttcgacgt agtggcacag gcgtctcgct
     7621 tttcgaagta gtggcacagg cgtctcgcct gtggtcgcag gagatatcgt gctcaaggtt
     7681 cctctcgttc tctgtgcggc cctgatcgcg ctcgccccct ccccgttgct ggccggcgag
     7741 caggcctcct cggcccgcgt cgtggtcacc gtcctcgacc agaccggcgc cgtgatcttg
     7801 caggcgccgg tctcgctgac ccccgaggac gggggcgcgg aagcagccgt gccccgagag
     7861 gcgctctcga acggactcgg ggttgcggcc ttcgacaatc ttccggaggg gcgctatacc
     7921 gttcgcgccc agttccccgg gttccagcag gcgaccgtga cgggggtgcg cgcccgccgc
     7981 ggccgcgaag cgcgcgtgcg ggtcgtgctg cagctcgaga agctcgacga gaacctcacg
     8041 gtcggccgcg accgccagac ctccgcgctc gatccgcgcg ggtcggcatt cagctcggtg
     8101 ctgacccgcg agcagatcga cgcgctgccc gacgatcccg acgagatgga agcggtgctg
     8161 aaggcgctcg ccccgcccgg ctcggtgatc cgggtggacg gcttcagcgg cgggaggctg
     8221 ccgccgaagt cgcagattcg ttcgatccgc ctgcctcgac tggacacctt cgccgcccag
     8281 aaccacggcg gcatgatggg tgcgcacttc atcgacatca tgacgatgcc cggaaacggg
     8341 ccgctgcggg ggaacgtcga cttcaacttc caggacgagg cgctcaacgc gcgc
//
LOCUS       DAOPQX010000150         8368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9503_length_8368_cov_4.210514, whole genome shotgun sequence.
ACCESSION   DAOPQX010000150 DAOPQX010000000
VERSION     DAOPQX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9503_length_8368_cov_4.210514"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PKK95_09295"
     CDS             complement(<1..873)
                     /locus_tag="PKK95_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOC18451.1"
                     /translation="MRALTGLLVLAFTVGLTGQGYQPTWESIDRRPTPSWFTDAKFGI
                     FIHWGVYSVPAYAPVVPGKLAYAEWYWNAMTRGRDDPKASEIQTGTWAYHQKMYGADF
                     PYQEFAKQFRAELFDPDHWADVFARSGARYVVLTSKHHEGFALWPSKEASAIWGRPWN
                     AVEIGPRRDLLGDLTEAVRRKGLRMGYYYSLYEWYNPVWLADKPRYVREHVFPQFKDL
                     VTRYRPSIIFSDGEWDLPSAGWRSPELLAWLKFPGQEAIVRLMRKIPPESVRPFEMRL
                     FRDLCRDGGPWLTAL"
     gene            complement(1229..2377)
                     /locus_tag="PKK95_09300"
     CDS             complement(1229..2377)
                     /locus_tag="PKK95_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18452.1"
                     /translation="MLTTYRVVTHVHSAGSNRLANRSYPLISSFLAREVGQAAQGLPW
                     FECETTLADLEEVLAGRRTSRPVDLLFLTDHVSARRHVVDEEALALARRCHRFGVGAE
                     IQTVLSDRCGGWIDAPEVLVYGPAGPHDGPLGRHYGITQALLDDLYDCCTPLGAPAPD
                     TVAVHGFCEARRLACALAHPLDGHDLRLEQLLALIGLFDVVEVVNGGFSQRSARLLER
                     LLAARRRENERRRSPAHEAGAGLPAALALGGSDAHVDDFDRVVTLFAATHRPTAGDFI
                     AAMLAARRDPSVRERFAIEGRGIATLTLYREVMTLVVRNIRRLSPCLPGLGLQARVLI
                     RGVLAASSELRRLDRSAARLQAELCDWLDREEGGLDPAPWHLAADLLR"
     gene            complement(2381..3121)
                     /locus_tag="PKK95_09305"
     CDS             complement(2381..3121)
                     /locus_tag="PKK95_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOC18453.1"
                     /translation="MPPPDYRLMFKLKDSVTTLSLLVSVASIVLAFDGRIALASFLVF
                     VAWGFDALDGLVARLTNTRNQFGAQFDDLADHVAYTLAPGFLAFAALRPYQFWAAAAA
                     GFATILLGTVRLARANTLALRYPGYWIGIPRPASGFVIVLLLNARLLAGEQWVWLQAA
                     AVILVAAGGLTRWPYKNHKGRLRSLERAFPIVGVASSLASYPAGWMWDVGLFWCAAYL
                     LAPWVAVSPAERRAIALALEHGTAGGAP"
     gene            complement(3129..4748)
                     /locus_tag="PKK95_09310"
     CDS             complement(3129..4748)
                     /locus_tag="PKK95_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18454.1"
                     /translation="MPTRSLARGLCAVPGFVLLACLLRLAFAPFGLVLAEDGFGYLVK
                     AREIAGGDLLPMSTHAIGWPLALAPFVGMAGGTHEAGTMAIARLVSIAAGTAILLPLA
                     RLARELVDPASMRLALLFTAVSAVLITSAWSAMAEPLFTLLLVGAVASMVWARGAART
                     PWWAGALAGLAWWVRPHGLLAAFALVLAAATERREGERLAAPLRRAAAVGLVACAVAA
                     PAAIQRHAAFGSPLTFGANDKILLAAEKEHVWSPNVPVVSLRDFLGSMTPGRAFDRFV
                     VDGAGRVAVQFTLYVATPLLVPFVLAAAWRRRHDPAFRPVLVVTGVWLAGVAGLAGVG
                     TRHLYPIAPLALLMAADGVRAAAARSRVRPAVVAAFVVVIVAQSLVTAAWHRHRETAA
                     EVADAVEWGRWAAGHVRGRLAIVEGSAFVMMHVPDTKVAGVGLMDLYAPATGLSVVRP
                     GTFDTLAQAMAWMRSAGVTHLAVDDLNIERRPYLKAVRDPAARPGCLRLVYSNEATAI
                     AWRQRIFEIRWRECDSCEVSAQSFRRSSDPR"
     gene            4831..6162
                     /locus_tag="PKK95_09315"
     CDS             4831..6162
                     /locus_tag="PKK95_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC18455.1"
                     /translation="MTCALAILGGGPAGLAAGCAAASLNVPFAVFEGSPHAGGNCRTI
                     SRDGFRFDTGAHRLHDRDAGVTAWVRSLLDGELTEVAAPSRIAFDGRFVNFPLSPINA
                     ARALGARRSARALIEIANQRLHPRTPAASFEDFALRSYGRTLAEAFLLGYSSKLWGRP
                     CRELAPEIAGARLNGLGVRAVLAEMAGGGRRTRHLDGRFLYPRRGIGRLTDRLAQACG
                     EALRLRSRITRVLHDGRRIAAVVVNDGERVDVEGAVSTLPLGAFASMLDPAPPPAVLD
                     AAEGLRFRHVVLVAIFLRRQSVTPCATVYFPDSAVPFTRVYEPRNRSEAMAPAGHTSL
                     VAEIPCGEADAPWRMDDLAVAALARGALEGIGWVDPAEIAGIDVHRLADAYPVLDVDA
                     RGRALAVEQFLGRFANLRLAGRPGRFAYSHLHDQLRQGRESVAAILGGANP"
     assembly_gap    6192..6201
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6202..7141
                     /locus_tag="PKK95_09320"
     CDS             <6202..7141
                     /locus_tag="PKK95_09320"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOC18456.1"
                     /translation="RPETVVLAAFVAVLAGLRLAYGGPLTADKLTAKVPLAGMGFIMA
                     AGVLADRLAWARGHAGSLPGRAARPWPAAARLASDWLPVVLCILVYENLHDVVKLIHH
                     DTLDPQLARIDAWMFGVQPTLWLQRLASPWLTDGLALAYSSYFFTPTILGVLLYAAGR
                     TREFRAFMLAVIAAMYLGFLGYVLVPAIGPLYYLEGQYDAPARLAGLWFYDAAHAFMN
                     DYRSINRDCFPSLHTAISALTLAWAWRARALHPLGRTVAWVYLPLTLALWFSTVYLRY
                     HWVIDLIAGWALAAIVMVTVPRIARWWHDAAEGGTS"
     gene            7138..>8368
                     /locus_tag="PKK95_09325"
     CDS             7138..>8368
                     /locus_tag="PKK95_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18457.1"
                     /translation="MNAAWALALALAALVLRLPVFLRTFASGDEATYSALAGALLDGR
                     WLYSGAVDHKPPLIASTYALVYAGAGRGAIHAVHALSILTLVATAFLIGVLARRLGLG
                     RAAARAAAVVYVIWASIGPGRDVLAANGELLMALPAVSALVLAASPSVLEGAGRFLVR
                     DSWRWFAAGSLAAVAALFKYQGAAIAAPLVLLAVSTEGARPGQRAARILARLSAAGLG
                     ALAVVAAFVVPYSLRGEIDALGFWAWDYPLRYAGSLDPASALFAGVGMTVWWLAMSAG
                     PLVLVAAGGTSFPARRLVIAWIAGAALGVASGGRFFLHYYLQLLPPLALAAGLGVARL
                     LERHSPASRRRLAAAATLTSAAVAISWGVNAAEFRLHPDRARHDAAYRAVGAYVTSRT
                     VSGEGIFVWGNSPEIYHY"
BASE COUNT         1142 a         3088 c         2984 g         1144 t
ORIGIN      
        1 cagggccgtc agccagggtc cgccgtcccg gcacaggtcc cggaacagcc gcatctcgaa
       61 cgggcgcacc gattccggcg ggatcttccg catcagccgg acgatggcct cctgccccgg
      121 gaacttgagc cacgcgagca gctcggggct tcgccacccg gccgagggca ggtcccactc
      181 gccgtcgctg aagatgatcg acggccggta gcgcgtcacc aggtccttga actgcgggaa
      241 gacgtgctcg cgcacgtatc gcggcttgtc cgccagccac accgggttgt accactcgta
      301 gagcgagtag tagtacccca tccgcagccc cttgcgacgg accgcctcgg tcaggtcgcc
      361 cagcaggtcg cgtctcgggc cgatctcgac ggcgttccac ggccggcccc agatcgccga
      421 cgcctccttg ctgggccaca gcgcgaaccc ctcgtggtgc ttggaggtga ggacgacgta
      481 gcgggcgccc gagcgcgcga agacgtcggc ccagtgatcc gggtcgaaca gctcggcgcg
      541 aaactgcttc gcgaactcct ggtaggggaa gtcggcgccg tacatcttct ggtgataggc
      601 ccacgtgccg gtctggatct cgctggcctt cgggtcgtcg cggccgcgcg tcatcgcgtt
      661 ccagtaccac tcggcgtagg cgagcttgcc cggcaccacc ggagcgtagg ccggcaccga
      721 gtacacgccc cagtggatga agatgccgaa cttggcgtcg gtgaaccacg aaggcgtcgg
      781 ccgcctgtcg atcgactccc acgtgggctg gtagccctgc cccgtcaggc ccacggtgaa
      841 cgcgaggacg aggagccctg tcagcgctct catgccacac cccttccagg gtcgggtgcc
      901 gcgacgcgtc acgctctcac gacccgcccg ccagccggca ttctatccgt ttcgcggtcc
      961 accccgaagg gatgcacggc ccgtcacgct gcgatcgact cccacgatcg gaccacaaag
     1021 gacacaaagg gcacaaagag gcgaaggctc gcgttcgatg gctctcaagg cgacaagaga
     1081 cccgtggcgc aacgctgccg gccatcggac ggtcgtcccg gagcatgtcg agggacgacc
     1141 gcgtcggggc cgccgacggc cggcagccgt ccccacgcgc cctgcgcagt ctttgtgatc
     1201 tgtgtggtcg atcggtcgtg atcgtggttc agcgcaacag gtccgcggcc aggtgccagg
     1261 gggccgggtc gaggccgcct tcctcgcggt cgagccagtc gcacagctcg gcctgcaggc
     1321 gtgccgcgga ccggtccagc cggcgcagct cgctcgaggc ggccagcacg ccgcggatca
     1381 gcacgcgggc ctggaggccg agccccggga ggcaggggct caggcgccgg atgttcctca
     1441 cgaccagcgt catcacctcg cggtacagcg tgagggtggc gatgccgcgg ccctcgatcg
     1501 cgaaccgttc gcgcaccgag gggtcgcgcc tggccgcgag catcgcggca atgaagtcgc
     1561 cggccgttgg gcggtgcgtc gcagcgaaga gcgtgacgac ccgatcgaag tcgtcgacgt
     1621 gtgcgtccga gccgcccagc gccagtgccg ccggcagccc cgcgccagcc tcgtgcgccg
     1681 gcgagcgcct ccgctcgttc tcgcgccgcc tggccgccag caggcgctcc agcagccgcg
     1741 cgctccgctg ggagaagccg ccgttcacga cctcgaccac gtcgaacagg ccgatcaggg
     1801 cgagcagctg ctcgagccgc aggtcgtgcc cgtcgagcgg gtgtgccagc gcgcaggcga
     1861 ggcgccgcgc ctcgcagaag ccgtggaccg cgacggtgtc cggcgccggc gctcccagtg
     1921 gcgtgcagca gtcgtagaga tcgtcgagca gcgcctgcgt gatcccgtag tgacgcccga
     1981 gcgggccgtc gtgcggccct gcgggcccgt acaccagcac ctcgggggca tcgatccacc
     2041 cgccgcatcg gtcggacagg acggtctgga tttcggcgcc gacgccgaag cggtggcacc
     2101 gccgtgcgag ggccagcgcc tcctcgtcca ccacgtgccg tcgcgcggag acgtggtccg
     2161 tcagaaagag caggtcgacg ggcctgctcg tgcggcgccc cgccagcacc tcctcgaggt
     2221 cggccagggt cgtctcgcac tcgaaccagg gaaggccttg ggccgcctga cccacctcgc
     2281 gcgccaggaa cgacgagata agcgggtagg accggttcgc gaggcggttc gatccggccg
     2341 agtgaacgtg cgtgacgacg cgataggtgg tgagcatcgg ctagggcgcg ccgcccgcgg
     2401 tgccgtgctc cagcgcgagg gcgatggccc ggcgctcggc gggcgagacg gccacccacg
     2461 gggcgagcag gtaggcggcg caccagaaca ggccgacgtc ccacatccag cctgccgggt
     2521 aggaggcgag gctcgaggcg accccgacga tcggaaaggc acgctcgagc gagcggaggc
     2581 ggcccttgtg gttcttgtag ggccagcgcg tcagcccgcc ggccgcgacc aggatcacgg
     2641 cggccgcctg caaccacacc cactgctccc cggccagcag cctcgcgttg agcagcagga
     2701 cgatgacgaa gcccgacgct ggccgcggga tgccgatcca gtacccggga tagcgaagcg
     2761 ccagcgtgtt cgcccgagcc aggcggacgg tccccagcag gatcgtggcg aatccggcgg
     2821 cggcagcggc ccagaactgg tacgggcgca gcgccgcgaa ggcgagaaag cccggcgcga
     2881 gcgtgtaggc cacgtggtcg gcgaggtcgt cgaactgggc gccgaactgg ttccgcgtgt
     2941 tcgtcaggcg cgccaccagc ccgtcgagcg cgtcgaaccc ccacgcgacg aacacgagaa
     3001 acgacgccag ggcgatgcgg ccatcgaacg cgagcacgat cgaggcgacc gacaccagca
     3061 gcgagagagt cgtgaccgaa tccttgagct tgaacatgag gcggtagtcg gggggcggca
     3121 cgcgaatctt agcgtggatc cgacgatcga cggaaggact gcgccgaaac ctcacacgaa
     3181 tcgcactccc gccagcggat ctcgaagatc cgctggcgcc aggcgatcgc ggtcgcctcg
     3241 ttcgagtaga ccagccgcag gcaccccggc cgggcggccg ggtcgcggac cgccttcagg
     3301 tacggccgcc gctcgatgtt gaggtcgtcc accgccaggt gtgtgacccc cgccgagcgc
     3361 atccacgcca tcgcctgtgc cagcgtgtcg aaggtccccg ggcgaacgac gctgaggccg
     3421 gtcgccggcg cgtagaggtc catcagtccg accccggcca ccttggtgtc gggcacgtgc
     3481 atcatcacga aggcgctgcc ctccacgatc gcgagccggc cgcggacgtg cccggctgcc
     3541 cagcggcccc actccacggc gtccgcgacc tcggccgccg tctcgcggtg acggtgccac
     3601 gcggcggtga cgagcgactg ggcgacgatc accaccacga acgccgcgac cacggccggc
     3661 cgaacccggc tgcgcgcggc ggcggcgcgc acaccgtcgg ccgccatcag cagcgcgagc
     3721 ggcgcgatcg ggtacaggtg gcgcgtgccg acccctgcca gccccgccac gccggccagc
     3781 cacacgccag tcaccacgag caccggcctg aaggcgggat cgtggcgccg tctccatgcc
     3841 gctgcgagca cgaacggcac gagcagcggc gtcgcgacgt acagcgtgaa ctgcaccgcc
     3901 acgcgtcccg ccccgtccac gacgaaccgg tcgaaggcgc ggccgggagt catcgacccc
     3961 aggaagtccc ggagcgagac caccgggacg ttcgggctcc agacgtgctc cttctcggcg
     4021 gcgaggagga tcttgtcgtt cgcgccgaag gtcaacggcg aaccgaaggc cgcgtgccgc
     4081 tggatcgccg cgggcgcggc caccgcgcac gcgaccaggc cgacagccgc cgcgcgccgc
     4141 agcggcgccg ccaggcgctc cccctcgcgc cgttcggttg ctgccgccag cacgagggcg
     4201 aacgccgcca gcagcccgtg cggcctgacc caccaggcca gcccggcgag cgcgccggcc
     4261 caccacggcg tgcgggcggc gcccctcgcc cacaccatcg acgccaccgc cccgaccagc
     4321 agcagcgtga agagcggctc ggccatcgcc gaccacgccg aggtgatgag gaccgccgag
     4381 acagccgtga acaggagcgc caggcgcatg ctcgcgggat cgaccagctc gcgcgccagg
     4441 cgggccagcg gaaggaggat cgcggtgccg gcggcaatcg acacgaggcg cgcgatcgcc
     4501 atcgtcccgg cctcgtgcgt gccgccggcc atccccacga acggggccag cgccagcggc
     4561 cagccgatgg cgtgggtcga catcggcagc aggtcgccgc cggcgatctc gcgcgccttc
     4621 acgaggtagc cgaacccgtc ctccgcgagc acgaggccga agggcgcgaa cgccaggcgc
     4681 agcaggcagg cgaggaggac gaacccgggg acggcgcaga ggccgcgtgc gagagagcgg
     4741 gtgggcatcg ggctcgttga ggtcgtggtc gatcgaggct accatcttac gcacccgcta
     4801 cgcccccctc aagcggcggc cttcatcgaa atgacctgcg cactcgcaat cctcggcggc
     4861 gggcccgccg gcctggcggc cggctgcgcc gcggcctcgc tgaacgtccc gttcgccgtg
     4921 ttcgagggct ccccgcacgc gggaggcaac tgccggacca tcagccgcga cgggttccgc
     4981 ttcgacaccg gggcgcatcg cctccacgac cgcgacgccg gcgtcaccgc gtgggtccgg
     5041 tcgctgctcg acggcgagct gaccgaggtc gcggcgccaa gccggatcgc gttcgacggg
     5101 cgcttcgtga acttccccct ctcaccaatc aacgcggcgc gggcgctggg ggcccgccgc
     5161 tcggcccggg cgctcatcga gatcgccaat cagcgcctgc atccgcgaac gcccgcggcg
     5221 tcgttcgagg acttcgccct gcggtcgtac gggcggacgc tggccgaggc attcctgttg
     5281 ggctactcgt cgaagctctg ggggcggccc tgccgggaac tggcccccga gatcgcggga
     5341 gcccgcctga acgggctcgg agtgcgggcg gtcctcgccg agatggcagg cggcggccgg
     5401 cgcacccgcc acctcgacgg gcgatttctc tacccccggc gcggcatcgg gcgcctcacc
     5461 gatcggctgg cccaggcctg cggggaggcc ctgcggctcc gctcgcggat cacccgcgtg
     5521 cttcacgacg ggcggcggat cgccgccgtg gtcgtcaacg acggcgagcg cgtggacgtc
     5581 gagggggccg tcagcaccct cccgctcggc gcgttcgcgt cgatgctcga cccggccccg
     5641 ccaccggcgg tgctcgacgc ggcggagggg cttcgcttcc gccacgtcgt gctggtcgcg
     5701 atcttcctgc gtcgccaatc ggtgacaccc tgcgccacgg tctatttccc cgactctgcg
     5761 gtgccgttca cgcgcgtgta cgagccccgc aaccgaagcg aggcgatggc gccggccgga
     5821 cacacctcgc tggtggcgga gatcccgtgc ggcgaggcgg acgcgccctg gcggatggac
     5881 gacctcgccg tcgccgcgct cgcgcgcggg gcgctcgagg ggatcgggtg ggtcgatccc
     5941 gccgagattg cgggcatcga cgtgcatcgc ctggccgacg cgtacccggt gctcgacgtc
     6001 gacgctcgcg gccgcgcgct ggccgtcgag cagttcctcg gccgcttcgc caaccttcgg
     6061 ctcgccgggc gtcccggacg attcgcctac agccacctgc acgaccagct gcgccagggc
     6121 cgcgagagcg ttgccgcgat cctcgggggg gccaacccgt gaaggcccgg gccgtggcgg
     6181 cgctcgtgcg gnnnnnnnnn nccgtcccga gacggtcgtg ctcgcggcct tcgtggcggt
     6241 cctggctggc ctgcggctgg cttacggcgg gccgctgacg gccgacaagc tgacggccaa
     6301 ggtgccgctc gcgggaatgg ggttcatcat ggccgccggc gtcctcgccg acaggctggc
     6361 gtgggcgcgc gggcacgccg gatcgctgcc ggggcgcgcg gcgcggccgt ggccggccgc
     6421 cgcacgcctg gcctccgact ggctgccggt ggtgctctgc atcctggtct acgagaacct
     6481 gcacgacgtc gtgaagctga tccaccacga cacgctcgat ccgcagctcg cgcggatcga
     6541 tgcctggatg ttcggggtgc agcccacgct ctggctgcag cgcctggcga gcccgtggct
     6601 caccgacggg ctcgccctgg cgtactcctc ctacttcttc acgcccacga ttctcggggt
     6661 gctgctgtac gcggcgggcc gcacgcggga gtttcgggcg ttcatgcttg ccgtgatcgc
     6721 ggcgatgtac ctgggattcc tgggatacgt gctcgtgccg gcgatcgggc ccctgtatta
     6781 cctggagggg cagtacgacg ccccggcgcg gctggccggg ctgtggttct acgacgcggc
     6841 gcacgcgttc atgaacgact accggtcgat caaccgcgac tgcttcccga gcctgcacac
     6901 cgcgatcagc gcgctcacgc tcgcgtgggc gtggcgggcg cgggctctcc atccgctggg
     6961 gcggacggtc gcctgggtgt acctgccgct cacgctggcg ctctggttct cgacggtcta
     7021 cctccgctac cactgggtca tcgacctcat cgccggctgg gccctcgcgg ccatcgtcat
     7081 ggtgacggtc ccgcgcatcg cacgctggtg gcacgacgcg gccgagggcg ggacgtcgtg
     7141 aacgccgcgt gggcgctggc cctggccctc gcggcgctcg tgctgcggct gccggtcttc
     7201 ctccggacct tcgccagcgg cgacgaggcc acctactcgg cgctggccgg cgcgttgctg
     7261 gacgggcggt ggctgtactc gggcgcggtg gatcacaagc ctccgctcat cgcctcgacc
     7321 tacgccctgg tctacgcggg ggctggccgc ggcgccatcc acgccgtcca cgcgctctcg
     7381 attctcacgc tcgtcgcgac cgcgttcctc atcggcgtgc tcgcccggcg actcgggctc
     7441 ggacgggcgg cggccagggc ggccgcggtt gtctacgtga tctgggcctc gatcggcccg
     7501 ggcagagacg tgctggcggc caacggcgag ctgctgatgg cgctgccggc cgtgtcggcc
     7561 cttgtcctgg ccgcatcccc gtcggtgctc gaaggggcgg gcaggttcct cgtgcgcgac
     7621 tcctggcgct ggttcgcagc agggagcctt gcggccgtcg ccgcgctgtt caagtaccag
     7681 ggagcggcga tcgcggcgcc cctcgtgctg ctcgccgtct cgaccgaggg cgcacggccg
     7741 gggcagcgtg ccgccaggat cctcgcccgt ctctcggccg ccgggctcgg cgccctcgcc
     7801 gtcgtcgccg cgttcgtcgt cccctactct ctccgcggcg agatcgatgc gctcggcttc
     7861 tgggcgtggg actacccgct gcggtacgcc gggtcgctcg accccgcgtc agccctgttc
     7921 gccggcgtgg gcatgaccgt ttggtggctg gccatgtcgg ccgggccgct ggtgctcgtc
     7981 gccgccggag ggacgtcgtt tcccgcgcgc cggctcgtca tcgcgtggat tgcgggcgcg
     8041 gccctcggcg tcgcctcggg cggacggttc ttcctgcact actacctcca gctgctgccg
     8101 ccgctggccc tggcggccgg cctgggcgtg gcgcgcctgc tcgagcggca ctcccccgct
     8161 tcgcggcgcc ggctcgccgc ggccgccacg ctgacctccg ccgcggtggc catctcgtgg
     8221 ggcgtcaacg ccgccgagtt ccggctgcac cccgaccgcg cccgccacga tgccgcctac
     8281 cgcgcggtgg gcgcctacgt cacgtcgcgc accgtttcgg gcgagggcat cttcgtctgg
     8341 ggcaactcgc ccgagatcta ccactacg
//
LOCUS       DAOPQX010000151         8367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9506_length_8367_cov_4.443816, whole genome shotgun sequence.
ACCESSION   DAOPQX010000151 DAOPQX010000000
VERSION     DAOPQX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9506_length_8367_cov_4.443816"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKK95_09330"
     CDS             complement(<1..428)
                     /locus_tag="PKK95_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOC18458.1"
                     /translation="MKRRDFLRAGTLAAGAVVAGPAGAAGPAAPQASGSRLAIPSFEL
                     EEATIADLQRAMETGRETARSLVEKYLSRIEALDRQGPALRQVLEVNPDALDLASRLD
                     EERKGGRTRGPLHGIPILVKDNVGTSDRMTTTAGSLALEGS"
     gene            484..1722
                     /locus_tag="PKK95_09335"
     CDS             484..1722
                     /locus_tag="PKK95_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC18459.1"
                     /translation="MLFRGLVNGIPVTGSLKENSLSLAEDERLVVSWDLGGRLYSVWR
                     FKHTWRRGLNGEVLYKWYEEGAGGPASRGYDERRRRTLTREEAAPVVDEAARFASRAL
                     AAMQEMPGSWSGRENPGVWSLFLQRLEACARFNSGVADADAARFAELYRPVGILPPDQ
                     YLSVVLQATEGCSFGSCTFCDLYHDGYRVKAPGEFAAHVRGVVEYLGASIALRRRSIF
                     LGAANALAVPVPRLVALLETLRATFPAPLPPVRAFVDAFTGVRKNTEDYRVLAGLGLG
                     RVYIGLESGHDPLLAFVRKPGTAGEAVETVRAMKAAGLQVGVIVMIGLGGEAFSSPHV
                     EDTARTVNAMGLGEGDLLYFSDLVEMPGTAYPAIARDAQVRPLDLAARLAQLSALRSR
                     LAFTGRPPQYARYDIREFVY"
     gene            2091..2669
                     /locus_tag="PKK95_09340"
     CDS             2091..2669
                     /locus_tag="PKK95_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOC18460.1"
                     /translation="MAYAAKDYSALIGMPGFSEALLKNHFTLYQGYVANTNKVIDAVE
                     ALAKEGKGASPEYAEQKRRLGWEFDGMRLHEYYFENLGGQGPLAPDGRLAQRIAADFG
                     SYEQWETDFKATGAMRGIGWAVLYQDSNTGRLLNFWINEHDTGHPAGCTPILVMDVFE
                     HAFMLDYGTRRADYIAAFFGNVNWAAAESRLR"
     gene            2677..3327
                     /locus_tag="PKK95_09345"
     CDS             2677..3327
                     /locus_tag="PKK95_09345"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOC18461.1"
                     /translation="MNYPIWSLPAPGLLIAAVAILHVFISHFAVGGGLFLVLAERRAR
                     RTADEAFLGYLKRHSRFFVLLTLVLGAITGVGIWFTIGLVHPQATSALINTFVWAWAI
                     EWTFFVTEIAAALVYYYGWERLSPRTHVAVGWIYFGAAWLSLAVINGILSFMLTPGAW
                     LETGSFWAGLANPTYWPSTFARTFVAIGLAGLYALLTAARLEDQALKARVARYAGT"
     gene            3395..4015
                     /locus_tag="PKK95_09350"
     CDS             3395..4015
                     /locus_tag="PKK95_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18462.1"
                     /translation="MTSLRETGVLRAARWTCLPEPATDPVARMEAEGREVFRLLCRSC
                     HTTGGYLAIRPLVARVSWAAAATILNFLPGFWWLVALPREALLALLGHEPLAAFCFAA
                     GVLASLGALALLVPAAFAPEPRRLLYGADGSLGLALVLMVLVRDSARRIALAPTGFGT
                     TAWVVPQWGPIAVFGALLVAAAVAVVWMVVKLARGTPARSPHDAGV"
     gene            complement(4039..4740)
                     /locus_tag="PKK95_09355"
     CDS             complement(4039..4740)
                     /locus_tag="PKK95_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOC18463.1"
                     /translation="MRIQTVFLDAGGVLVVPNWRRIADTLAAHGVETTPEALARADPH
                     ARFDLDVTKGRNASDQQRGWLYFNLVLEKAGVALTHRTEAALAELHAYHEQVNLWEQV
                     PDGVVEALDRMRRAGLRLVVVSNSNGRLRVLFDRLGLSRKVDAMLDSQEEGVEKPDRR
                     LFDLALERTAAARESTLHAGDLYYVDVEGARAAGLRAVLIDPLGLYRDFDCERVASLG
                     ELADRLLEAGLTPES"
     gene            complement(4811..5284)
                     /gene="trxA"
                     /locus_tag="PKK95_09360"
     CDS             complement(4811..5284)
                     /gene="trxA"
                     /locus_tag="PKK95_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOC18464.1"
                     /translation="MSAPSPSLDARGVIVACPSCGQRNRIPYAHLHSEVRCGKCGTGV
                     RAPGQAIEVPDVETFDAVVSRASLPVVVDYWAEWCGPCRMVTPELAKVAQQNAGRLLV
                     VKVNTEELPEVAARFRVQSIPMLGVFRNGEVAGETVGARPASDIQAFIDQALAGP"
     gene            complement(5281..6039)
                     /locus_tag="PKK95_09365"
     CDS             complement(5281..6039)
                     /locus_tag="PKK95_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOC18465.1"
                     /translation="MKIATWNVNGIRARQEQVHSWIALEQPDIVCLQELKASSDQLPA
                     FLCELEGYWCYWHGAKGYSGVGLHLRKAAFPERPLFTHPPFDFENRIVVTRLGGTVVG
                     SVYVPNGGKDFPAKMKFLASLEDYAREAAGQGLQLVLCGDLNVARTDMDVHPKERKVN
                     AIGQLPEERALLERIISRGLVDVGRALDPENDQLFTWWAPWRNLRQRNIGWRLDYVLA
                     SGSLAQRARSCPVFREVGTSDHAPVLADFDTEQL"
     gene            6083..>8367
                     /locus_tag="PKK95_09370"
     CDS             6083..>8367
                     /locus_tag="PKK95_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18466.1"
                     /translation="MPAGVSVACRRLLTLAALLAVPAAAWAAGQSRTELAQWTIQSSA
                     RVPERGDRLSRPGVDVQSWHAVTVPTTVVGALVEAGVYKDPYFGMNLRAIPGTTYPIG
                     ERFTLLPTPADSPFKPAWWYRTEFTVPREKAGRRLALHFDGINYRANVWLNGRQVAAS
                     SDVVGTFRRFEFDVTGIARAGVNALAVEVFGPEPEDLAIMWVDWNPTPADKNMGLWGK
                     VYLTDSGPVTLRHPYVETALELPSLKAARLTVSAELANQTDSPVAATLRGQIGAIAFS
                     KPVSLAPRSTATVVFTPEEVPGLTMTDPRVWWPYRYGDQPLYTLTISVESGGTTSDRA
                     ETRFGIHEYGSELTPEGHRRFTVNGKPILVRGAGWSSDMLLRPPPPGRLEAELRYVRE
                     MGLNTIRLEGKLESDEFYDLADRMGILLMPGWCCCDQWEQWKKWDAEDHRVGPASLES
                     QLLRIRNHPSVLVWLNGSDFPPPADVERNYLDVARRCRWRKPVLSNATDAPGPISGPS
                     GVKMRGPYDYVPPSYWLLDTKNGGAFGFATEVGPGAAVPPIESLRRMLPPEHLWPIDE
                     VWRYHAGGDEFKDLSLFTGALEGRYGKATSAEDYARKAQALAYEGQRAMFEAFGRNKY
                     LATGVIQWMLNNAWPSMIWHLYDWYLRPGGGYFGTKAACEPLHVQFSYDDRSVAVVND
                     TLQPRTGLTVSAEVFDPNLRELFAREAAVEVAADAVTRAFALPAPSAGWSKTYFVRLA
                     LRDNAGREVSRNFYWLSTEEDR"
BASE COUNT         1191 a         2944 c         2900 g         1332 t
ORIGIN      
        1 gagccctcga gcgccagcga tcccgccgtc gtcgtcattc ggtcggaggt gccgacgttg
       61 tccttgacga ggatcgggat gccgtggagc gggccccgcg tgcggccgcc cttgcgctcc
      121 tcgtcgaggc gcgaggcgag atcgagcgcg tcggggttga cctcgagcac ctggcgcagc
      181 gcgggtccct gccggtcgag cgcctcgatg cgggacaggt atttctcgac cagcgagcgc
      241 gcggtctcgc gcccggtttc catcgccctt tgcagatcgg cgatggtggc ttcctccagc
      301 tcgaaggatg gaatggcgag ccggcttccc gacgcttgcg gcgctgctgg cccggcggcg
      361 cccgccggcc ccgccaccac cgcacccgcc gccagcgtcc ccgcccggag gaagtctcgt
      421 cgtttcatga cccgaatagt aacctgtacc gggcacaatg cctgccccta caggtgacag
      481 gtcatgcttt ttcgtggtct cgtcaacggc atccccgtga ccggcagcct gaaggagaac
      541 tcgctgtcgc tggctgagga cgagcgtctc gtcgtctcct gggacctggg cggccgtctg
      601 tacagcgtgt ggcgcttcaa gcacacgtgg cggcgcgggc tgaacggcga ggtgctctac
      661 aagtggtacg aggagggcgc gggcgggccg gccagccggg gctacgacga gcgccggcgg
      721 cggacgctga cccgcgaaga agcggcgccg gtagtagacg aggccgcgcg gttcgcctcc
      781 cgggcgctgg cggcgatgca ggagatgccc ggctcctgga gcgggcggga gaacccgggt
      841 gtctggagcc tcttcctgca gcgcctcgag gcgtgcgcac ggttcaactc gggggtggcg
      901 gacgccgacg cggcgcgctt cgccgaactc taccggccgg tcgggatcct gccgcccgat
      961 cagtacctgt ccgtggtgct gcaggccacc gaggggtgct cgttcggcag ctgcacgttc
     1021 tgcgacttgt accacgacgg ctaccgggtg aaggcacccg gcgagttcgc cgcccacgtg
     1081 cgcggcgtcg tggagtacct cggggcgtcg atcgcgctgc ggcggcgctc catcttcctg
     1141 ggggcggcca acgccctggc cgtcccggtg cctcgcctcg tcgcgctgct cgagacgctg
     1201 cgcgcgacgt ttccggcgcc gctgccgccg gtgcgcgcct tcgtggatgc ctttaccggc
     1261 gtgcgcaaga acaccgaaga ctaccgggtg ctggcgggcc tgggcctcgg ccgggtctac
     1321 atcggcctcg agtcgggtca cgacccgctg ctcgccttcg tccgcaagcc gggcaccgcg
     1381 ggtgaggcgg tcgagacggt ccgcgcgatg aaggccgcgg ggctccaggt cggcgtgatc
     1441 gtgatgatcg gcctcggcgg agaggcgttc tcctccccgc acgtcgagga caccgcaaga
     1501 accgtgaacg cgatggggct cggagagggc gacctgttgt acttcagcga tctggtggag
     1561 atgcccggca cagcctaccc tgccatcgcg cgtgacgcgc aggtgcggcc gctcgacctg
     1621 gccgcgcgtc tcgcccagct ctctgccctt cgctcgcgcc tggcgttcac cggccgcccg
     1681 ccgcagtacg cccgctacga catcagggag ttcgtgtact gacggagatc gcatcgtgca
     1741 ggaggtgcag gtggtgccga aggtgccgaa ggcctgccgc gccgtagcgc gcaccccgcg
     1801 gcgaaggcgg gtgcagtgct gatggtgccg gaagtgctgg tgccgagagt gccgcgctcc
     1861 ctctgcgccg ttcagtgcgc cagcctgtca cgtacggttc accgcagacg aaacctccgc
     1921 gtgcgtctcc gtgcctctgt gcctctgtgg tgaacgtgga caagccgcga cgtctgtcgc
     1981 gtacgatgca acgccgcgta tatggtagag ttgcgccgtt ccaccttgat ccgtcgaggc
     2041 acaagccgtc cgccgggact gtcccgtggg acgtttcagg aggcactgcg atggcttacg
     2101 cggcgaaaga ctactcggcc ctcatcggca tgccgggctt cagcgaggcc ctgctgaaga
     2161 accacttcac gctctaccag ggctacgtgg ccaacacgaa caaggtgatc gacgcggtcg
     2221 aggcgctcgc gaaggaaggg aagggagcgt cgcccgagta cgccgagcag aagcggcggc
     2281 tgggatggga gttcgacggc atgcggctgc acgagtacta cttcgagaac ctgggcgggc
     2341 aggggccgct cgcgcccgac ggcaggctgg cgcagcggat cgcggccgac ttcggcagct
     2401 acgagcagtg ggagaccgac ttcaaggcca ccggcgcgat gcggggcatc ggctgggcgg
     2461 tcctgtacca ggactcgaac acggggcggc tgctcaactt ctggatcaac gagcacgaca
     2521 ccgggcaccc cgccgggtgc acgccgatcc tcgtgatgga cgtgttcgag cacgccttca
     2581 tgctcgacta cggcaccagg cgcgccgact acatcgccgc cttcttcggc aacgtgaact
     2641 gggccgccgc cgagtcgcgc ctgcggtagc ggccgcatga actatcccat ctggtcgctg
     2701 ccggcgccgg ggctgctcat cgccgccgtg gcgatcctgc acgtgttcat ctcgcacttc
     2761 gcggtcggcg ggggcctctt cctggtcctc gccgaacgca gggcgcggcg cactgcagac
     2821 gaggcgttcc tgggctacct gaagcggcac agccgcttct tcgtcctcct cacgctggtg
     2881 ctcggagcga tcaccggggt cggaatctgg ttcacgattg gcctggtcca cccgcaggcc
     2941 acctcggcgc tcatcaacac cttcgtctgg gcctgggcga tcgagtggac gttcttcgtc
     3001 accgagatcg ctgccgccct cgtctactac tacggctggg agcgcctgtc gccgcgaacc
     3061 cacgtggcgg tcggctggat ctacttcggc gccgcctggc tcagcctggc agtcatcaac
     3121 ggcatcctca gcttcatgct gacgccgggc gcctggctcg aaaccggcag cttctgggcg
     3181 ggcctggcca accccaccta ctggccctcg acctttgcga gaaccttcgt ggcgatcggc
     3241 ctcgccgggc tctacgcgct gctgaccgca gcgcgtctcg aggaccaggc tttgaaggcg
     3301 cgcgtggcgc gctacgccgg aacctgacgg cgtgcgcgtg ccgaggccgg cggctgcgcc
     3361 ggccccgccc gatgccgaac cctatccgtt cacggtgacc tcgctgcgcg agaccggcgt
     3421 gcttcgcgcg gcgcgctgga cctgtctgcc cgagccggcc accgaccccg tcgccaggat
     3481 ggaggcggag ggacgcgagg tgttccgcct gctgtgcagg tcgtgccaca cgaccggcgg
     3541 ctacctggcg atccgcccgc tcgtggcgcg ggtctcctgg gcggccgcgg cgaccatcct
     3601 gaacttcctc cccggcttct ggtggctggt cgcgcttccc cgcgaggcac tgctcgcgct
     3661 gctcgggcac gagccgctcg ccgccttctg ctttgcggcc ggcgtcctgg cgtcgctggg
     3721 cgcgctcgcg ctgctcgtgc cggcggcctt cgctcccgag ccgcggcgcc tgctctacgg
     3781 cgctgacggc tcgctcggcc tcgcactcgt gctgatggtg ctggtgcgcg actcggcccg
     3841 ccggatcgcg ctggccccga ccgggttcgg gacgaccgcc tgggtggtgc cgcagtgggg
     3901 gccaatcgcg gtcttcggcg ccctgctggt ggccgcggct gtcgcggtgg tctggatggt
     3961 agtcaagctc gcgcgcggaa cgcccgcccg ctcgccgcac gacgcgggcg tgtaggggga
     4021 ggattccgga gtcaggattc aggattcggg agtcaggcca gcttccagca gcctgtcggc
     4081 cagctcccca agcgacgcaa cccgctcgca gtcgaagtcg cggtacaggc caaggggatc
     4141 gatgagcacg gcgcgcagcc ccgccgcgcg cgctccctcc acgtccacgt agtacaggtc
     4201 gcccgcgtgc agcgtcgact cgcgcgcggc ggccgtgcgc tcgagcgcca ggtcgaacag
     4261 ccggcggtcg ggcttctcca ctccctcctc ctgggaatcg agcatcgcgt ccaccttgcg
     4321 actgaggccc aggcgatcga acaggacgcg gagcctgccg ttggagttcg acacgaccac
     4381 cagccgcagc ccggcccggc gcatccggtc gagcgcctcg accacgccgt cggggacctg
     4441 ctcccagagg ttgacttgct cgtgataggc atggagctcg gcaagcgcgg cctcggtccg
     4501 gtgcgtgagg gcgacaccgg ccttctcgag gaccaggttg aagtacagcc agcctcgctg
     4561 ctggtcgctg gcgttcctcc ccttggtcac gtccagatcg aaccgggcgt gggggtctgc
     4621 ccgcgccagc gcctcgggcg tcgtctccac gccgtgcgcc gccagggtgt cggcgatccg
     4681 ccgccaattg ggaaccacca gcacgccgcc agcatcgagg aagaccgttt ggattcgcat
     4741 ccctgtcgtc ctagcagacc gctgtgtttc tacattcttc cttttgcatt ttgcattcag
     4801 gccgcgcttc tcaggggcct gccagcgcct ggtcgatgaa cgcctggatg tcgctggccg
     4861 ggcgcgcgcc caccgtctcg ccggccacct cgccgttcct gaacaccccc agcatcggga
     4921 tcgactgcac ccggaagcga gcggccacct cgggcagctc ctccgtgttc actttgacca
     4981 ccagcaggcg cccggcgttc tgctgcgcca ccttggccaa ttcgggcgtc accatccggc
     5041 aggggccgca ccactcggcc cagtagtcca ccacgaccgg gagcgaggcg cgtgacacga
     5101 cggcgtcgaa cgtctcgacg tccggcacct cgatcgcctg tcctggggcc cgcaccccgg
     5161 tgccgcactt gccgcaccgt acctcgctgt gaaggtgagc gtagggaatc cggtttcgct
     5221 gcccgcagct cgggcacgcg acgatcaccc cgcgggcgtc gagcgagggg gaaggggcgc
     5281 tcatagctgc tccgtgtcga agtcggccag caccggggcg tggtcggacg tccccacctc
     5341 gcggaacacg gggcaggacc gtgcccgctg ggcaaggctc ccgctggcca gcacgtagtc
     5401 gaggcgccac ccgatgttgc gctggcggag gttccgccac ggcgcccacc aggtgaacaa
     5461 ctggtcgttc tccgggtcga gcgcgcggcc gacgtccacg aggccgcgcg agatgatccg
     5521 ctcgagcagc gcgcgctcct ccgggagctg gccgatcgca ttcaccttcc gctccttcgg
     5581 gtgcacgtcc atgtcggtcc gcgccacgtt cagatcgccg cacagcacga gctgcagccc
     5641 ctgccccgcg gcctcgcgcg cgtagtcctc gagcgacgcc agaaacttca tcttcgccgg
     5701 gaagtccttg ccgccgttgg ggacgtacac cgatcccacc accgtgccgc ccaggcgggt
     5761 gacgacgatc cggttctcga agtcgaaggg cgggtgggtg aacagcggcc gctcggggaa
     5821 ggccgccttc cgcaggtgca gcccgacgcc cgagtacccc ttggcgccgt gccagtagca
     5881 ccagtagcct tccagctcgc acaggaacgc cggcagctgg tcggacgagg ccttcagctc
     5941 ctgcaggcag acgatgtcag gctgctccag ggcgatccag ctgtggacct gctcctggcg
     6001 ggcgcggatg ccgttgacgt tccaggtcgc gatcttcatc ggtttcagtg tcatcatagc
     6061 gcgtcgatcg aaggaggaac ccatgcctgc aggggtgagc gtcgcctgcc gccgtctgct
     6121 cacgctcgcc gcgctcctgg ccgtgccggc ggcggcgtgg gccgcaggac agtcgcggac
     6181 cgaactggcg cagtggacca tccagtcgag cgcgcgcgtg cccgagaggg gggatcgcct
     6241 gtcgcgcccc ggggtggacg tccagtcgtg gcacgccgtc acggtgccca ccaccgtcgt
     6301 cggcgcgctg gtggaggccg gcgtctacaa ggatccgtac ttcggcatga acctgcgcgc
     6361 catcccgggg accacctacc cgattggcga gcggttcacg ctgctgccga cccccgccga
     6421 cagccccttc aagccggcgt ggtggtaccg caccgagttc accgtccccc gggagaaggc
     6481 cgggcggcgc ctggcccttc acttcgacgg gatcaactac cgggcgaacg tgtggctgaa
     6541 cggccggcag gttgccgcct cctccgacgt ggtggggaca ttccggcggt tcgagttcga
     6601 cgtgacgggg atcgcccggg cgggggtcaa cgcgcttgcg gtggaggtct tcgggcccga
     6661 gcccgaagac ctcgccatca tgtgggtgga ctggaacccc acgccggcgg acaagaacat
     6721 gggcctctgg ggcaaggtct acctgaccga cagtggcccc gtgaccctgc gccatcccta
     6781 cgtcgagacc gctctcgagc tgccctcgct gaaagctgcg cggctcacgg tcagcgccga
     6841 gctggccaac cagacggaca gcccggtggc ggcgacactc cggggccaga tcggggccat
     6901 cgcgttcagc aagccggtgt cgctcgcccc ccggtccacc gcgacggtcg tcttcacgcc
     6961 cgaggaagtg cccggcctca ccatgaccga cccgcgggta tggtggccgt accgctacgg
     7021 cgatcagccg ctctacacgc tgacgatcag cgtggagagt ggcggcacga cgtccgatcg
     7081 ggctgagacc cgcttcggca ttcacgagta cggctcggag ctgacgcccg aagggcaccg
     7141 gcgtttcacg gtcaacggga agccgatcct cgtccgcggc gcggggtggt cgtccgacat
     7201 gctgctccgg ccgccgccgc ccgggcggct ggaggcggag ctgcgctacg ttcgcgaaat
     7261 ggggctcaac accatccggc tcgaggggaa gctcgagagc gacgagttct acgacctggc
     7321 cgaccggatg ggcatcctgc tgatgcccgg ctggtgctgc tgcgaccagt gggagcagtg
     7381 gaagaagtgg gatgccgagg accaccgcgt gggccccgcg tcgctcgaga gccagctcct
     7441 gcgcatccgc aaccacccga gcgtcctggt ctggctgaac ggcagcgact tcccgccccc
     7501 cgccgacgtc gagcgcaact acctcgatgt cgctcgacgg tgccggtggc gaaagccggt
     7561 gctctcgaac gccaccgatg cccccggccc gatcagcggc cccagcggcg tgaagatgcg
     7621 cggcccgtac gactacgtcc ctccttcgta ctggctcctc gacacgaaga acgggggggc
     7681 cttcggcttt gccaccgagg tgggccccgg cgccgccgtc ccgccgatcg aaagcctgcg
     7741 caggatgctg ccgcccgagc acctctggcc gatcgacgag gtgtggcgct accacgcggg
     7801 gggcgacgag ttcaaggacc tgtcgctctt cactggcgcg ctggaggggc gctacggcaa
     7861 ggcgacgagc gccgaggact acgcccgcaa ggcgcaggcg ctcgcctacg agggacagcg
     7921 cgcgatgttc gaggcgttcg gacggaacaa gtacctggcg accggcgtga tccagtggat
     7981 gctgaacaac gcctggccgt cgatgatctg gcacctgtac gactggtact tgcggcccgg
     8041 cggcggctat ttcggcacca aggcagcctg cgagccgctc cacgtccagt tctcgtacga
     8101 cgaccgctcg gtggccgtcg tgaacgacac gctccagccg cgaacgggac tgacggtctc
     8161 ggccgaggtg ttcgatccga acctgaggga gctgttcgcg cgtgaggcag cggtggaggt
     8221 tgccgccgac gcggtaacac gggccttcgc gctgcccgcg ccgtctgccg ggtggagcaa
     8281 gacctacttc gtgcgcctcg ccctgcgcga caacgcgggg cgggaagtga gccgcaactt
     8341 ctactggctc tccaccgagg aggatcg
//
LOCUS       DAOPQX010000152         8358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9518_length_8358_cov_4.863062, whole genome shotgun sequence.
ACCESSION   DAOPQX010000152 DAOPQX010000000
VERSION     DAOPQX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9518_length_8358_cov_4.863062"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKK95_09375"
     CDS             complement(<1..763)
                     /locus_tag="PKK95_09375"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOC18467.1"
                     /translation="MTHARHTDALPLSILPLFALLALIALPSPASSQTVVDPRIVEFS
                     PSADHDTTLPDGRPAVERYLLKVFQAGASQPFHTVDMGKPDADADGTVRFDFWSTVAA
                     WPLPGGNYVSRVDAVGPGGNGLSAPSNPFTFGSSCSYGLVAPTSASFPAAGGGGSVSV
                     DAGDECDWTATASAGWIRLAAGGGTGSGSVIFNVDPNPNTTPRTGTIDVAGSTFTVTQ
                     AGVTCTYTVSPASASVAASAGSGSCSTTGSVGAVDA"
     assembly_gap    1166..1265
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1266..1528)
                     /locus_tag="PKK95_09380"
     CDS             complement(<1266..1528)
                     /locus_tag="PKK95_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18468.1"
                     /translation="MATSSPCFVRPLPFTMAASSSSDFPVRFWMFFNWVASALSRSGM
                     RTLLSAGRQPPVQLRYGGPRSLHHTAEVGMGSGLKAEDRLKAQG"
     gene            1559..1822
                     /locus_tag="PKK95_09385"
     CDS             1559..1822
                     /locus_tag="PKK95_09385"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HOC18469.1"
                     /translation="MRAFGDCEAAPKKTYVSYRRKKQFAMIGPATNTRVEVGLNAKAL
                     PASDRLAQLPAGQMCNYKVRIGDPSEVDAELIGWMKTAYDAAG"
     gene            2444..2578
                     /locus_tag="PKK95_09390"
     CDS             2444..2578
                     /locus_tag="PKK95_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18470.1"
                     /translation="MIRSRSSGTSGLRRTGGTGWRLRMASIVSVALCPRKGRAPVAIS
                     "
     gene            complement(2839..4083)
                     /locus_tag="PKK95_09395"
     CDS             complement(2839..4083)
                     /locus_tag="PKK95_09395"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC18471.1"
                     /translation="MSDSSFRLAFDECRGLLQRRLAEPAPGRVQLLTGPRQVGKTTLL
                     LELADRVGPGAVYAAADAPEAALPGFWERTVARVEQAAAEHRGAVLLLDEAHLLADWA
                     GRLKGAWDSFRRRRIPVHVVATGSSALRLSSGSRESLAGRFERLTLAHWSAASLARAF
                     GFAPRKAAEQLVVRGSYPGAVPLLGDPARWTAYVRDAIVEPAIGRDLLALTAIRRPAL
                     LRQVFAVATASPARIVSLQKIQGQLQDPGALETLAHYLALLEEAFLVAGLQKYSARGA
                     RRRAAPPKLIALNNALLAVMPSAGPADPRADAAVFGAWVENACLAHAWNAGQAVGYWR
                     EEPLEVDGVLEGSWGNWAIEVKTGELRTGELAGLLEFTRRHPKFRPLVVCGPADVGRA
                     RSWGISALAWQDFLLLEGPPRA"
     gene            4231..5085
                     /locus_tag="PKK95_09400"
     CDS             4231..5085
                     /locus_tag="PKK95_09400"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC18472.1"
                     /translation="MKCARFLLAVLLVSFLAVPAAQSPDDTKIWQEFVAWLNAQPDVT
                     QIGPDRYKAKLLADGLTPAQADERIAALGRLTPQHRDEIGAIYFNKLYATPSQTRFTL
                     QPNAFLVEMTKGLKPGAALDAGIGQGRNAVYLAAQGWDVTGYDVAEDGLKIAEQNAVK
                     AGTSIKTVKARFEDFDYGTDRWDLIYFVYVDAPITDPAFIAKVSAGLKKGGVLLIDRP
                     FHPIDKPEPGWPPPTEQDKVNALPRAWSNLQLVRYDDTFGIADWQQTREAREVKQLRL
                     VKMLARKW"
     gene            complement(5245..6138)
                     /locus_tag="PKK95_09405"
     CDS             complement(5245..6138)
                     /locus_tag="PKK95_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOC18473.1"
                     /translation="MPTTTLPPGAEAYQAKDYKSELKPIWCPGCGDFGVVQAIYRALA
                     AIGRPPHEIAFISGIGCSSRIPGYTTAYGFNTVHGRALPIAQGIKLANPQLLVLAAGG
                     DGDGFSIGGGHIAHAVRRNIDLTYIIMDNQIYGLTKGQLSPTSPRGLRTASSSYGSME
                     DPVNPLLYVLGYGCRFVAQGTPADMAQLGSLIEEGIRFPGFAFINVQSPCVTYGEPDS
                     QLKVQKQRMRTLESLGHDPTDRIKAMDLAQHYATDLYTGVFYRDPNPPPHFGEYVAER
                     QANIGAARERERVLDLFQPAS"
     gene            complement(6268..8055)
                     /locus_tag="PKK95_09410"
     CDS             complement(6268..8055)
                     /locus_tag="PKK95_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOC18474.1"
                     /translation="MTTDLILGMAGSGGDGIVSAGDSLMTALALSGYHAMLTKSFGPQ
                     IRGGESSFRLRVSTGPVLGPGGALDVAVALNWDDFLKFGAELSLDGRSIVIYDTNTKV
                     APDQIPVAGVKPAEVVAVGIEAMARETAGTDKAKNTVVLGLMAGWFGLAPETVRAGLR
                     KRFAKKGEEVVARNEKAFEAGFAWAQANPLRTGQALAPMPAGTSAKLVADGNDLCAAA
                     AIVAGCEFFGGYPITPSSEIMHFLNREIWKYGGVMLQCEDEMAGIGACVGASFAGKKA
                     LTATSGPGMSLKTEILGLASIAELPLVVVNVQRGGPSTGIPTKSEQSDLFQAAFSAHG
                     DAVRPVLAPIDVADTFPITVEAFNIAERYQTPVIILSDQEIAQRKEAIERPATHGLKI
                     ETRRTPTEAELEHYVRFRFTESGVSPISHPGMKGGNYLASGIEHTETGAPTATGSVHA
                     KMNEKRLHKLAPLKSRDDLFVAYGPSNAPLALVAWGSVSGIAREALDLSREAGLKVKL
                     LVPRLLYPVAEEVYRDFFASVQGGFVVEQSHQGQLYRVIRMFVDVPRGVEPFARSGSN
                     PFSPGEIVDRLRAKARALVEERQTVNQAE"
BASE COUNT         1249 a         2922 c         2850 g         1237 t
ORIGIN      
        1 cggcatcgac ggcgccgacg ctgccggtgg tcgagcacga gccgctgccc gccgatgccg
       61 cgaccgacgc cgaagccggc gacaccgtgt aggtgcaggt cactccggcc tgcgtgacgg
      121 tgaaggtcga tccggcgaca tcgatcgtgc cggtgcgcgg cgtggtgttc gggttcggat
      181 cgacgttgaa gatgaccgat ccgctgccgg ttcccccgcc ggcggcgagg cggatccatc
      241 ccgccgacgc cgtcgccgtc cagtcgcact cgtcgcccgc gtccaccgaa accgatccgc
      301 cgccgcccgc tgccgggaac gacgccgagg tcggcgccac caggccgtaa ctgcacgagg
      361 agccgaacgt gaacgggttc gacggggcgc tcaggccgtt gccgccgggg ccgaccgcgt
      421 ccacgcgcga cacgtagttg ccgccgggaa gcggccacgc ggccaccgtg gaccagaagt
      481 cgaacctgac ggtcccgtcc gcgtcggcgt cgggcttccc catgtcgacg gtgtgaaacg
      541 gctgcgaggc tcccgcctgg aacaccttca acaggtagcg ctcgacggcg ggacgcccgt
      601 ccgggagcgt cgtgtcgtgg tcggccgagg ggctgaactc gacgatccgc ggatcgacaa
      661 cggtctgcga agaagcagga gacggcagcg cgatgagtgc gagaagcgcg aacaggggca
      721 gtatcgacag gggaagcgcg tcggtgtgtc gcgcgtgggt catgttccct tccttcttca
      781 cggactctca ggccacacgc atggcggccc gcgcgcacgc ctgacgcgag gagagcgctg
      841 gctatgggga gaagcgtgtc gggtcgtcat tgggggggcg attggcaggc gcggcgcacg
      901 caccgggact gccgtccgtg tctcgggccc tgcacgatca ggacccgcgc gggcggccgt
      961 gaagatggag tattttgcca ggaaggcgcc ggatgagctt ggaggttccc cgccggctga
     1021 gatcacgatc ctggcgcccg gatttcgatc tggagcgccc gcctccgcca agcgctcgct
     1081 acgttcccgc cttcgccaag cgcgcggcta cggcgggcag gctacggctt cggcgggcag
     1141 gcccttgagc cgtgagccgt gagccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnccttg agccttgagc cgatcttcag ccttgagccc tgagcccatt cccacctcgg
     1321 cggtatgatg gagtgacctg ggtccaccat accgaagctg cacgggcggc tgccgcccgg
     1381 ccgagaggag tgtccgcatg cccgatctcg acaaggcgct cgccacccag ttgaagaaca
     1441 tccagaagcg gacggggaag tcgctcgacg agctggcggc catcgtgaac gggagcggcc
     1501 tgacgaagca cggcgagctg gtggccatgc tcaagaccac gctcggctgc tcgaggcgat
     1561 gcgcgcgttc ggcgactgcg aggccgcgcc gaagaagacc tacgtcagct atcgccgcaa
     1621 gaagcagttc gcgatgattg gccctgccac caacacgcgc gtcgaggtcg gcctcaacgc
     1681 gaaggcgctg cccgcctccg accggcttgc gcagctgccc gccgggcaga tgtgcaacta
     1741 caaggtgcgg atcggcgacc cttcggaggt ggacgccgag ctgatcggct ggatgaagac
     1801 ggcgtacgac gccgcggggt gaggggggtc gtaccgaaag ggtgagattc ttcacccccg
     1861 gggtcgtacc caaaccgtgg cggccttcac accccggggg gtcgtaccca aaaggtgaag
     1921 tccttcacgc gtctggtacg acccccgccg gcctgtcatg gaaagcccag ggcgccgata
     1981 gaccgggcac ccgaccctcg gagctcgccg tgatcagacg caccgtcttc gcgctcgccg
     2041 ttcttgccct cgttaaattc gccctcgcgg tccttgtccc cgccgtcgtc gctcaccccg
     2101 ttcttgctca cgccgtcgct gcccccgccg ccggaaccgc attcgccggc gatctgcccg
     2161 gctcgggccg tccgccgcag cgccctttct cgggcgtcgt ctcgcacgtg gcggacggcg
     2221 acacgctcga cgtgtcagtt gggggccgca tccccgccgg gggccgcacc gtgaccgtgc
     2281 gcctcgaggg gatcgacgcg cccgaggggg gggccgccgt tcagcgtcga ggcccggcgc
     2341 cacctccgcg tcctcgcatt cggtcagcgg gtgaccgtcg tccccagcga caccgaccgc
     2401 cgactggtag cggacccacg ggtccttctc gagcgccttc gcgatgatcc gctcgaggtc
     2461 gtcggggacg tcggggttga ggcggaccgg gggcacgggc tggcggttga ggatggcgtc
     2521 gatcgtctcg gtggcgctct gcccgcggaa ggggagggcg ccggtcgcca tctcgtagag
     2581 gacgacgccg aaggagaaga ggtcgctgcg cgcgtccacc gtcccgccga gcgcctgctc
     2641 cggcgccatg tacgacaccg tgccgagcgt cgttcccacg gcggtgacct cgtccacgtg
     2701 cgtcatcgtc gggcggtcgg cgtcgtcttc ttcgtccgcc gggccggcga ggcgggtcgg
     2761 ggcactgtcg cctgcggaag cggcgccggc cgtcctcttg gcgagtcacg ttttcggtac
     2821 ggccccctct cgggaacgct acgcgcgcgg cggcccctcc agcagcagga aatcctgcca
     2881 cgcgagggcc gagattcccc agctccgcgc ccgccccacg tcggccggcc cgcacaccac
     2941 gagaggtcgg aacttcgggt ggcggcgggt gaactcgagc aggccagcca gttcgccggt
     3001 tcgcagctcg cccgtcttca cctcaatcgc ccagtttccc cagctcccct cgagtactcc
     3061 gtccacctcg agcggttcct cccgccagta gcccaccgcc tgccccgcgt tccacgcgtg
     3121 cgcgaggcag gcgttctcga cccaggcccc gaacaccgcc gcgtccgcgc ggggatcggc
     3181 ggggccggcg gacggcatca ccgccaggag cgcgttgttc agtgcgatca gcttcggggg
     3241 cgcagcccgg cgccgcgcgc cgcgcgcgga gtacttctgc aggccggcaa cgaggaaggc
     3301 ttcctcgagc agcgccaggt agtgcgccag ggtctccagc gcaccgggat cctgcagctg
     3361 tccctggatc ttctgcaacg agacgatgcg cgcgggagat gccgtcgcca cggcaaacac
     3421 ctggcggagc agggcggggc gccggatcgc ggtcagcgca agcaggtcac ggccaatcgc
     3481 cggctcgacg atcgcgtccc tgacgtacgc cgtccaccgg gcgggatcgc cgagcagcgg
     3541 cacggctccg gggtacgacc cgcgcaccac gagctgctcg gctgccttcc gaggggcgaa
     3601 accaaaggcg cgggccaggg aggcggccga ccagtgggcc agcgtgagac gctcgaagcg
     3661 gcctgcgaga ctttcgcgcg agccgctcga caagcggagg gcggacgacc ccgtggcaac
     3721 gacgtggacc gggatgcgcc gacgccggaa cgaatcccac gcgcccttca accgtcctgc
     3781 ccagtcggcg aggaggtggg cctcgtccag gaggagcacg gcaccgcggt gctccgcggc
     3841 cgcctgctcc acgcgcgcaa cggttcgctc ccagaagccg ggcagggcgg cttccggggc
     3901 gtcggccgcc gcgtacacag cgcccggccc cactcgatcc gccagctcga gcagcagggt
     3961 cgtcttgccg acctgccggg gcccggtcag cagctgcacg cgcccggggg cgggctcggc
     4021 cagccggcgc tggaggaggc cgcggcactc gtcgaaggcg agcctgaacg aactgtcaga
     4081 catactcgaa taattattcg actatgatcg atagtagtca aggggccgcg gccgggccgc
     4141 gagccgtctt tccgtcctgg gccgtgagcc cctccgccct tccccggaac aatcgccggc
     4201 gcccagcgtc aaacgccctg gaggttccga atgaagtgtg cccgtttcct cctggccgtc
     4261 ctcctggtct ccttcctcgc cgtccccgcc gcccagtccc ccgatgacac gaagatctgg
     4321 caggagttcg ttgcctggct gaacgcccag cccgacgtca cccagatcgg ccccgaccgc
     4381 tacaaggcga agctgctggc cgacggcctg acgcccgcgc aggcggacga gcggatcgcg
     4441 gcgctcggcc ggctcacccc gcagcaccgg gacgagatcg gcgccatcta cttcaacaag
     4501 ctgtatgcga cgccgagcca gacgcgcttc accctgcagc cgaacgcctt cctcgtggag
     4561 atgacgaagg ggctgaagcc cggggccgcc ctcgacgccg gcatcgggca ggggcggaac
     4621 gcggtctacc tcgccgcgca gggctgggac gtcaccggct acgacgtcgc cgaggacggc
     4681 ctgaagatcg ccgagcagaa cgcggtgaag gcgggaacct cgatcaagac ggtgaaggcg
     4741 cgcttcgagg acttcgacta cgggacggat cgctgggacc tgatctactt cgtctacgtg
     4801 gacgccccga tcaccgaccc cgcgttcatc gcgaaggtgt cggccggcct caagaaggga
     4861 ggcgtgctgc tcatcgaccg gccgttccac ccgatcgaca agcccgagcc ggggtggccg
     4921 ccgccgaccg agcaggacaa ggtgaacgcg ctgccgaggg cctggtcgaa cctgcagctc
     4981 gttcgctacg acgacacgtt cggcatcgcc gactggcagc agacgcgcga agcccgcgag
     5041 gtcaagcagc tccgcctggt caagatgctc gcgcgcaagt ggtgagggca tcgcggcgct
     5101 ataatggcgc cggccgtgac gtcccccgct cctcttccgg ccgtccgtgt ctccgcgcga
     5161 gcgccgtggt gggtggtgaa aagccgcacc gcctcggtac gaccccgccg gcccttcgac
     5221 tcggggcggg cgactccgcc tcgctcacga cgccggctgg aacaggtcga ggacgcgctc
     5281 gcgctcgcgg gcggcgccga tgttcgcctg gcgctccgcg acgtactcgc cgaagtgggg
     5341 cggcgggttg gggtcgcggt agaagacgcc ggtgtagagg tcggtcgcgt agtgctgcgc
     5401 gaggtccatc gccttgatgc gatcggtggg gtcgtggccg agcgactcga gcgttcgcat
     5461 ccgctgcttc tgcaccttca gctgcgaatc gggctcgccg taggtgacgc acggcgactg
     5521 cacgttgatg aacgcgaagc cggggaagcg gatgccctcc tcgatgaggg agccgagctg
     5581 cgccatgtcg gccggcgtcc cctgcgcgac gaagcggcag ccgtagccca gcacgtagag
     5641 cagcgggttg accggatcct ccatcgaccc gtacgagctg gacgcggtgc gcagaccccg
     5701 cggcgacgtc ggggagagct gccccttggt caggccgtag atctggttgt ccatgatgat
     5761 gtaggtcagg tcgatgttcc ggcgcacggc gtgggcgatg tggccgccgc cgatggagaa
     5821 gccgtcgccg tcgcccccgg ccgcgaggac gagcagttgg gggttggcga gcttgatccc
     5881 ctgcgcgatc ggcagcgcgc gcccgtgcac cgtgttgaag ccgtaggcgg tagtgtagcc
     5941 ggggatgcgc gacgaacagc cgatgcccga gatgaacgcg atctcgtgcg gcggccggcc
     6001 gatcgccgcg agcgcccggt agatcgcctg caccacgccg aagtcgccgc agccggggca
     6061 ccagatcggc ttcagctcgc tcttgtagtc cttcgcctgg tacgcctcgg cgcccggcgg
     6121 cagcgtggtc gttggcatag ggtcccttcg cagtaatgcg tctgtcacgt acgattcacc
     6181 gcagaggcgc agagggcgca gagattcttc gaatgatcca agatctccgc atccgtctcc
     6241 gtgtctctgt gcctctgtgg tgaacgttta ttccgcctgg ttcacggtct ggcgctcctc
     6301 gacgagtgcc cgcgccttgg cgcgcagcct gtcgacgatc tcgcccggcg agaacgggtt
     6361 cgacccgctt cgcgcgaacg gctcgacgcc gcgcggcacg tccacgaaca tccggatgac
     6421 gcggtagagc tgcccctggt gcgactgctc caccacgaag ccgccctgca ccgaggcgaa
     6481 gaagtcgcgg tacacctcct cggccaccgg gtagagcagc cggggcacca gcagcttcac
     6541 cttcaaaccc gcctcgcggg agaggtcgag cgcctcgcgc gcgatccccg agaccgaccc
     6601 ccacgccacc agcgccagcg gcgcgttcga cgggccgtag gcgacgaaca ggtcgtcgcg
     6661 cgacttcagc ggcgccagct tgtgcagccg cttctcgttc atcttcgcgt gcaccgaccc
     6721 ggtggcggtg ggcgcccccg tctccgtgtg ctcgatgccc gacgcgaggt agttgccgcc
     6781 cttcatcccc gggtggctga tcgggctgac gcccgactcg gtgaagcgga agcggacgta
     6841 gtgctccagc tcggcctcgg tcggcgtccg ccgcgtctcg atcttcagcc cgtgcgtcgc
     6901 cggccgctca atcgcttcct tgcgctgagc gatctcctgg tccgacagga tgatgacggg
     6961 cgtctggtac cgctcggcga tgttgaaggc ctcgaccgtg atcgggaacg tgtcggccac
     7021 gtcgatcggc gcgagcaccg ggcgcaccgc gtcgccgtgc gccgagaagg cggcctggaa
     7081 caggtctgac tgctcgctct tggtggggat gccggtcgag gggccgccgc gctgcacgtt
     7141 caccaccacg agcggcagct cggcaatcga cgccaggcca aggatctcgg tcttgagcga
     7201 catgcccggg ccgctggtgg cggtgagcgc cttcttgccg gcgaagctcg cccccacgca
     7261 cgccccgatc cccgccatct cgtcctcgca ctggagcatc accccgccgt acttccagat
     7321 ctcccggttg aggaagtgca tgatctccga cgagggggtg atcgggtagc cgccgaagaa
     7381 ctcgcacccg gcgacaatcg ccgcggcggc gcagaggtcg ttgccgtcgg ccaccagctt
     7441 cgcggaggtg ccggcgggca tcggcgccag cgcctgcccg gtgcggagcg ggttggcctg
     7501 cgcccaggcg aagcccgcct cgaacgcctt ctcgtttcgc gcgacgacct cctcgccctt
     7561 cttcgcgaac cgcttgcgca gcccggcccg caccgtctct ggcgccaggc cgaaccaccc
     7621 ggccatcagc ccgagcacga ccgtgttctt ggccttgtcg gtgcccgcgg tctcgcgcgc
     7681 catcgcctcg atgccgactg ccaccacctc ggccggcttc acccctgcca ccgggatctg
     7741 gtcgggcgcg accttcgtgt tcgtgtcgta gatgacgatg gagcggccgt cgagcgagag
     7801 ctcggcgccg aacttgagga agtcgtccca gttgagcgcg accgccacgt cgagcgcccc
     7861 gccggggccg agcacgggcc cggtcgagac gcgcagcctg aaagacgact cgccgccgcg
     7921 gatctgcggc ccgaagctct tggtgagcat cgcgtggtag ccgctcagcg cgagcgcggt
     7981 catcagcgaa tcgccggcgg agacgatgcc gtcccccccg gacccggcca tgccgaggat
     8041 gaggtcggtg gtcatggatg tttactgaac atactccgga ctagtccacg aagtccacga
     8101 agtcgcgaag tcccgccttc gccgaacgct cgctgcactc gcggcttcgg cgggcaagcc
     8161 ccgaagtcag tccacgaagt cggggggtct acgagtcacg gagtcgactg accgcgcgcc
     8221 ccgccgcgca gcccacaaat cagctattct tgggctcgca caaatcaggt atcgggattc
     8281 aggaatcggg aatctcccac cgagaggccg ccatgagact ccgccacgcg ttcctgctcg
     8341 cctcggctct tgccgtcc
//
LOCUS       DAOPQX010000153         8341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9545_length_8341_cov_4.507000, whole genome shotgun sequence.
ACCESSION   DAOPQX010000153 DAOPQX010000000
VERSION     DAOPQX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9545_length_8341_cov_4.507000"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="PKK95_09415"
     CDS             <1..1124
                     /locus_tag="PKK95_09415"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOC18475.1"
                     /translation="QIEGRTIYLSSPEYVASYARRFIANGVRLVGGCCGTTPEHIRQI
                     KLAVGAAMLKPARPVSGAARAEVADAGVEPVPRDEKSRLAHAMARGTFVHLAQLDPPR
                     GHEAATVIEEARALKIQGVDAINVPDGPLSAARMSALSLAVLIEQQAGIETLMHYACR
                     ERSLLGMQADLLGAHAMGVRNVLLVTGDPPRRGDYAFPAGLHEVDSIGLTNVVSRLNR
                     GLDLGGERLERQTAYHIGVAVNPTALDLDEELRRLAYKVEAGAEFAVTAPVFDLRAFD
                     HFMDRAERFGIPIVAGIWPFDSALNAEFMANEVPGVAVPEALLRRMARADTRAAAMDE
                     GVAIAREIAVAVRPRVQGLQIGNASGRVARVLQVFDAVT"
     gene            1225..1932
                     /locus_tag="PKK95_09420"
     CDS             1225..1932
                     /locus_tag="PKK95_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC18476.1"
                     /translation="MALFEPGDRRPPDEYISSSDAATRALSVTAVENDFVARVYEKLS
                     SVYDWIFGPTLHPGRLQALQRMGIREGDRVLEVGVGTGINAALYPRTCSVTGVDLSES
                     MLEKARERVARKGLGNVRLLQMDAASLTFPDDAFDIVYAPYLVSVVPDPVQVAREMRR
                     VCRPGGRVVILNHFRSPNFLLSRAERLISPFTVHIGFKADLDLPAFLAQADLQPVSIE
                     KVNLPRIWSLVTCIKGS"
     gene            complement(2191..3306)
                     /locus_tag="PKK95_09425"
     CDS             complement(2191..3306)
                     /locus_tag="PKK95_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18477.1"
                     /translation="MRPVVWYISGHGFGHASRQIEIINAVRARRPGLPIVVRTGASPW
                     IFDLTLRSPVEVQHVECDTGVAQIDSLRLDEAETLRRAGEFYANFGRRVADEAVFLRD
                     AGASLVVADIPPLAIAAAREAGVPSVAIGNFTWDWIYEAYEPERAGLPHLVPAIREAC
                     IGASQYVRLPMWGGFEMAPDDRTVDLPLVARRSSRPRGETRASLGLPADRRLVLLSFG
                     GHGLENLDAAALRGLAPEYLVIVTGHLQLARLARAAGDAVAVFDERALYDAGWRYEDL
                     VAASDVVLTKPGYGIIAECAANGTAILYTSRGHFIEYEVLVQEMPLYVRSQFIPQEAL
                     FAGDWRPYLEELLAQDHPEPPDVSGADAAADLILQRL"
     gene            complement(3483..5900)
                     /gene="lon"
                     /locus_tag="PKK95_09430"
     CDS             complement(3483..5900)
                     /gene="lon"
                     /locus_tag="PKK95_09430"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOC18478.1"
                     /translation="MATPTTDALAQVPAELPVLPLRRSVLFPLTLQPLAVDRPVSIES
                     INRALAADRMLFLTMQQTDTDDPQPADLNRVGAVGVIRQMVKAPSGGVHIIVEGLSRA
                     AGDPITRQGLQMTARLSAMPEEREQSIEVDAYVRRLRELMDRAMNIASGLSQELRGIV
                     MSIDDPLRLAYLLGSLLDMKADDKQRLLEEDRLLVKLQAVAGVLNREIALLELKGKIE
                     SQAQQEMTDAQRQYYLRQQLKAIQEELGEKEGAEVSELRERIEKAGLPEHVAAVAERE
                     VDRLERMTPASPEYQMIRTYLDWILEVPWTATTPDRLDPVEARRVLDEDHYDLDKVKD
                     RIVEYLAVRKLKGDMKGPILCFVGAPGVGKTSLGQSIARAMNRKFVRISLGGVRDEAE
                     IRGHRRTYIGALPGRIIQALKQAGSANPVFMLDEIDKMSVGFQGDPAAALLEVLDPAQ
                     NNTFRDHYLEVNFDLSRVLFIATANQLGPVHPALLDRMEVISLAGYTEEEKVHIARRY
                     LIPRQLAEHGLPAGALDLPDETLRAVISQYTREAGVRNVERQIGTLARKVAARVATAK
                     SADAPGAEEQAEGPGAEGGTTNVEITPTRIEPGDLPEYLGPPRIHGEVAFRTSRPGVA
                     TGVAWTETGGDVLFVEATLLPGGKGNLTLTGQLGNVMQESARAAVSHIRANARELGVD
                     PAFLDGHDLHVHVPAGAIPKDGPSAGVTIATAILSAIRNQPVLQDVAMTGEITLSGLV
                     LPVGGIREKALAARRHGIRTFLLPERNQPDLSELPEEVRNTMTFVPASTIEDVLRVAL
                     PAPIPTS"
     gene            complement(5984..6262)
                     /locus_tag="PKK95_09435"
     CDS             complement(5984..6262)
                     /locus_tag="PKK95_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18479.1"
                     /translation="MPERRSRAWNPRATLAVAAVVVVTWNVVFDGRVKSAVWDYVDRQ
                     QRYVAGEAPPADIDAAMAAGVRRGLLEASGAAALAWALAAAVARRASR"
     gene            6291..6566
                     /locus_tag="PKK95_09440"
     CDS             6291..6566
                     /locus_tag="PKK95_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC18480.1"
                     /translation="MICKQCGTEIADKALVCYRCGAATFEPRVRPPQKRRRPSRLLLV
                     AAMVVLILAALFMATAAAGQAPPLLSWVIGALAVIVVVWFLFTRLRR"
     gene            6591..7304
                     /locus_tag="PKK95_09445"
     CDS             6591..7304
                     /locus_tag="PKK95_09445"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOC18481.1"
                     /translation="MRSLAVAIYAWGGGAAFAAALGYFAYTFGATMGRAVPAGTPGTG
                     VGAILIDVALFAAFAAHHSAYARERVKRGVARAVSPALERSTYVWVSSLLFILVCAAW
                     QPVGGTLYVHRGLLAWLHVPVAAAGVALTAMGAFRLRAFDLAGIAQARGEEGEAGRSL
                     VVAWPYTMVRHPIYLAWLLLVFSPPSMTATRFVFACVSSAYLVLAVPLEERLLIKRFG
                     EAYRGYRRQVRWRMVPGVY"
     gene            complement(7311..8255)
                     /locus_tag="PKK95_09450"
     CDS             complement(7311..8255)
                     /locus_tag="PKK95_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOC18482.1"
                     /translation="MTTRSRKTERSGAWMAAAALAVGAALGGTVCASSHQAPAASAGF
                     DGSKAYAHVREVVGFGPRPAGSAALEAARGYIKKQLAAAGVEVREQAFTADTPIGPVR
                     MVNLIATIPGRSPGKIVFGGHYDTKLFRAFRFVGANDAGSSTAFLIEWARVLKNRKNA
                     LTSEVVFFDGEEAFVDWYTDHDNTYGSRHYVEAARRDGSLKQIRAMVLVDMIGDRDLH
                     IRRELSSTRWLTDIVWAAARRLKHTDVFLDQTTQIEDDHVPFLRAGIPAVNIIDGPED
                     YPPWHTAGDTLDKVSARSLQVVGDVLLEALGGIEKKLQ"
BASE COUNT         1160 a         2903 c         2955 g         1323 t
ORIGIN      
        1 gacagatcga ggggcgcacg atctacctgt catcgcccga gtacgtcgcg tcgtacgcgc
       61 ggcggttcat cgcgaacggc gtccgcctcg tgggcggctg ctgcggcacc acgcccgagc
      121 acatccggca gatcaagctc gcggtcgggg cggccatgct gaagcccgcc cggccggtct
      181 cgggcgccgc ccgtgccgag gttgccgacg ccggtgtcga gccggtgccc cgcgacgaga
      241 agtcgcgcct ggcgcacgcg atggcgcgcg ggaccttcgt gcacctggcg cagctcgatc
      301 cgccgcgggg ccacgaggcg gccacggtga tcgaggaggc gcgcgccctg aagatccagg
      361 gcgtcgacgc gatcaacgtc cccgacggcc cgctgtcggc cgcccggatg agcgcgctgt
      421 cgctggccgt gctgatcgag cagcaggcgg ggatcgagac gctgatgcac tacgcctgcc
      481 gcgagcgcag cctcctcggc atgcaggccg acctgctcgg cgcccacgcg atgggggtgc
      541 ggaacgtgct gctggtcacg ggcgaccccc cgcgccgcgg cgactacgcc ttcccggccg
      601 gcctgcacga ggtggactcg atcggcctga ccaacgtggt gagccggctc aaccgcggcc
      661 tcgaccttgg cggcgagcgg ctcgagcggc agacggcgta tcacatcggc gtcgcggtga
      721 atccgaccgc gctcgacctc gacgaagagc tgcggcgcct ggcctacaag gtcgaggcgg
      781 gtgccgagtt cgccgtcacg gccccggtgt tcgacctgcg ggccttcgac cacttcatgg
      841 accgcgccga gcgcttcggg atcccaatcg tcgccggtat ctggcccttc gacagcgccc
      901 tgaacgccga gttcatggcg aacgaggttc ccggggtggc ggtgcccgag gcgctgctgc
      961 gccggatggc ccgcgccgac acccgggcgg ccgccatgga cgagggcgtg gcgattgcca
     1021 gggaaatcgc cgtggcggtc aggccccggg tgcaggggct gcagatcgga aatgcgtcgg
     1081 ggcgcgttgc ccgggtgctc caggtgttcg acgcggtcac ctaggatgag gaggctcacg
     1141 ctccgggcaa ggctggtata gtagtaggct tggccgtatg gcgcggaccg ccccgggggc
     1201 gggcgccggc ccatggagaa cggaatggcc ttattcgaac cgggtgacag gcggccgccc
     1261 gacgagtaca tctcctcgtc cgacgcggcc actcgggcgc tgtcggtcac agcggtcgag
     1321 aacgatttcg tcgcccgggt ctacgagaag ctgtcgtcgg tgtacgactg gatcttcggg
     1381 cccacgctcc acccgggccg gctgcaggcg ctgcagcgca tgggcatccg cgagggcgac
     1441 cgggtgctcg aggtcggcgt agggaccggc atcaacgcgg ccctgtaccc gcggacctgc
     1501 tcggtgaccg gcgtcgacct gtccgagtcg atgctcgaga aggcgcggga gcgggtggcg
     1561 cgcaaggggt tgggcaacgt acggctgctg cagatggacg ccgcgagcct gaccttcccc
     1621 gacgacgcct tcgacatcgt ctacgcgccg tacctcgtga gcgtcgtgcc cgacccagtg
     1681 caggtcgccc gcgagatgcg ccgcgtgtgc cgccccggcg gccgcgtcgt gatcctcaac
     1741 cacttccgca gcccgaactt cctgctttcg cgggccgagc ggctcatctc gcccttcacc
     1801 gtgcacatcg gcttcaaggc cgacctcgac ctgcccgcgt tcctcgcgca ggccgacctg
     1861 cagccggtgt cgatcgagaa ggtgaacctg ccccggatct ggtcgctggt gacctgtatc
     1921 aaagggagct aggaaccagg gctcggggcc aggacgccgg aaaccgtagg ggcggccctt
     1981 gtggccgccc tttttcgttg aacgatcacg ttaaccacag agacacagag acgcggagac
     2041 gtacgcagag atctcggatc ttctggtgac gcggagacgc ggagacgtac gcagagatct
     2101 cggatcttct ggtgattctc tgcgccctct gcgtctgcgg ttaacgtgat cgtgggacca
     2161 tgaatgcagc gtgacccgcg tgacaggcgt tcacaggcgc tgcaggatga gatcggccgc
     2221 cgcgtcggcc ccgctcacgt cgggaggctc gggatggtcc tgcgccagca attcctccag
     2281 gtacggccgc cagtctccgg cgaagagcgc ttcctggggg atgaactgcg accggacgta
     2341 gaggggcatc tcctggacca gcacctcgta ctcgatgaag tgtccccgtg aggtgtagag
     2401 gatggccgtg ccgttggcgg cgcactcggc gatgatgccg tatccgggct tggtcagcac
     2461 gacgtccgag gccgccacca ggtcctcgta gcgccagccg gcgtcgtaga gcgcgcgctc
     2521 gtcgaacacc gccacggcgt cgccggctgc gcgcgccagc cgggcgagct gcaggtgccc
     2581 ggtgacgatc accaggtact ccggggccag gccccggagc gctgcggcgt ccaggttctc
     2641 gagcccgtgg ccgccgaacg agagcagcac gagccggcgg tcggccggca gccccagcga
     2701 cgcccgggtc tcgccgcgcg ggcgggacga gcgcctcgcc acgagcggca ggtccacggt
     2761 gcgatcgtcg ggcgccatct cgaaaccgcc ccacatcggc aggcggacgt actgcgacgc
     2821 cccgatgcac gcctcccgga ttgccggcac gaggtgcggc aggcccgctc gctcgggctc
     2881 gtaagcctcg tagatccagt cccaggtgaa gttgccgatc gccaccgatg gcacgcccgc
     2941 ctcgcgcgct gcggcgatgg cgagcggcgg gatgtcggcg acgacgaggg aggcgccggc
     3001 gtcgcggagg aacaccgcct cgtcggccac ccggcgcccg aagttggcgt agaactcgcc
     3061 cgcccggcgc agcgtctcgg cttcgtcgag ccgcaggctg tcgatctgtg ccacgccggt
     3121 gtcgcactcc acgtgctgga cctccaccgg cgagcggagc gtcaagtcga agatccaggg
     3181 gctcgcgccg gttcggacga cgatcggcag gcccggacgc cgggcgcgga ccgcgttgat
     3241 gatctcgatc tggcgggagg catggccgaa cccgtgcccg ctgatgtacc acacgacagg
     3301 acgcatgcgg ggattctaat gcagagcacc gctgcggctg cgtacgcgac agacgcagcg
     3361 acttgtccac gttgaccaca gaggcacaga ggcacggaga cgcacgcaga gatctcggat
     3421 cattcgggga atctctgcgt cctctgcgtc tctgcggtgc acgtgatcaa gggtcgtcgc
     3481 ccctacgacg tggggatcgg cgccgggagc gcgacccgca gcacgtcctc gatcgtcgag
     3541 gcgggcacga acgtcatcgt gttccgcacc tcttcgggca gctcggagag atccggctgg
     3601 ttccgctcgg gcagcaggaa ggtgcggatc ccgtggcgcc tggccgccag cgccttctcg
     3661 cggatgccgc ccaccggcag caccaggccg ctcagcgtaa tctcgccggt catcgccacg
     3721 tcctgcagca ccggctggtt gcggatcgcc gacaggatcg cggtggcgat cgtgacgccg
     3781 gccgaggggc cgtccttcgg gatggcgccg gccggcacgt gaacgtggag gtcgtggccg
     3841 tcgaggaacg ccgggtcgac gcccagctcg cgcgcgttgg cccggatgtg gctcaccgcg
     3901 gccctcgccg attcctgcat cacgttcccg agctgccccg tcagcgtgag gttccccttg
     3961 cccccgggca gcagcgtcgc ctcgacgaag agcacgtcgc cgccggtctc ggtccaggcc
     4021 acgccggtcg cgaccccggg ccgcgaggtt cggaacgcca cctcgccgtg gatgcgcggc
     4081 gggcccaggt actcgggcag atcgccgggc tcgatgcgcg tcggcgtgat ctccacgttc
     4141 gtcgttccgc cctccgcccc cgggccctcg gcttgctcct cggcgcccgg cgcatcggcc
     4201 gacttcgccg tggccacccg tgcagccacc ttcctcgcga gcgtaccgat ctggcgctcg
     4261 acgttccgta cgccggcttc gcgggtgtac tgactgatga cggcgcgcag cgtctcgtcg
     4321 gggaggtcga gggccccggc agggaggccg tgctcggcca gctggcgggg gatgagatag
     4381 cgccgcgcga tgtggacctt ctcttcctcc gtgtacccgg cgagcgagat cacctccatg
     4441 cggtcgagga gcgccgggtg cacgggaccg agctggttcg cggtcgcgat gaacaggacg
     4501 cgggagagat cgaagttcac ctcgaggtag tggtcgcgga acgtgttgtt ctgcgccggg
     4561 tcgagcacct cgagcagcgc cgcggccggg tcgccctgga agccgacgct catcttgtcg
     4621 atctcgtcga gcatgaacac ggggttggcc gagcccgcct gcttcagcgc ctggatgatt
     4681 cggcccggca gcgccccgat gtaggtgcgc cggtggccgc ggatctcggc ctcgtcgcgg
     4741 acgcccccga gggagatgcg cacgaacttg cggttcatcg ctcgcgcgat cgactggccg
     4801 agcgaggtct tgccaacgcc gggcgccccg acgaagcaga ggatcggccc cttcatgtcc
     4861 cccttcagct tccggacggc caggtactcg acgatccgat ccttcacctt gtcgaggtcg
     4921 tagtggtcct cgtcgagcac gcgccgcgcc tcgacagggt cgaggcggtc tggcgtggtg
     4981 gccgtccacg gcacctcgag gatccagtcg aggtaggtgc ggatcatctg gtactcgggc
     5041 gaggccggcg tcatccgctc caggcggtcc acctcgcgct cggccaccgc cgccacgtgc
     5101 tcgggcagcc cggccttctc gatccgctcg cggagctcgc tgacctcggc cccttccttc
     5161 tcgcccagct cctcctggat ggccttgagc tgctgccgca ggtagtactg gcgctgcgcg
     5221 tcggtcatct cctgctgcgc ctgcgactcg atcttcccct tcagctcgag cagcgcgatc
     5281 tcgcggttca gcacgcccgc caccgcctgc agcttgacga gcaggcggtc ttcctcgagg
     5341 agccgctgct tgtcatcggc cttcatgtcg agcaggctgc cgaggaggta ggccagcctc
     5401 agcggatcgt cgatgctcat gacgatgccg cgcagctcct gcgagaggcc cgaggcgatg
     5461 ttcatcgcgc ggtccatcag ctcgcgcagg cggcggacgt aggcatccac ctcgattgac
     5521 tgctcccgtt cctcgggcat cgcgctcagc cgcgcggtca tctgcaggcc ttgacgcgtg
     5581 atgggatcgc cggcggcgcg cgacagcccc tccacgatga tgtgaacgcc gcccgagggg
     5641 gccttcacca tctgccggat gaccccgacg gccccgacgc ggttcaggtc ggcaggctgc
     5701 gggtcgtcgg tgtcggtctg ctgcatcgtg aggaagagca tccggtcggc ggccagcgcg
     5761 cggttgatcg attcgattga aacagggcga tcgacggcca gcggctgaag cgtcagcggg
     5821 aacagcaccg atcgccggag cggcagaacc gggagttcgg ccggcacctg cgcgagagca
     5881 tccgtcgtgg gggtagccat aggcctattg tcgcaccgac caagcttccg tgcggaaccg
     5941 cccggccatc ccggcggctg tgagcaggtg ccgcttggag ggctcagcgg ctggcgcggc
     6001 gggcaacggc ggcggccagc gcccaggcca gggcagccgc cccggacgcc tcgagcaggc
     6061 cgcgccggac gccggcggcc atcgcggcgt cgatgtcggc aggtggcgct tcgccggcca
     6121 cgtagcgctg ctggcggtcc acgtagtccc agacggccga cttcacccgg ccgtcgaaga
     6181 cgacgttcca ggtcaccacc accaccgccg ccaccgccag ggtggcgcgg ggattccacg
     6241 cgcgcgagcg cctctcgggc atcgcctgct agcgtatact caaccactgc atgatctgca
     6301 aacagtgcgg cacggaaatc gcggacaagg cgctggtctg ctaccggtgc ggggcggcca
     6361 ctttcgagcc gcgagtgcgc ccgccccaga agagacggcg acccagccgg ctgctgctcg
     6421 tggccgcaat ggtcgtgctc atcctggccg cgctgttcat ggcgaccgcc gcggcggggc
     6481 aggcaccgcc cttgctctcg tgggtgattg gggcgctggc ggtcatcgtc gtggtgtggt
     6541 tcctcttcac gcgcctccgt cgatagtcga gggccgctgc gagcggtgac atgcgcagtc
     6601 tggcggttgc gatctatgcc tgggggggcg gcgcggcctt cgccgccgcg ctcggctact
     6661 tcgcttacac cttcggcgcc acaatggggc gggcggtgcc ggcgggaacg ccgggcaccg
     6721 gcgtgggcgc aatcctgatc gacgtcgccc tcttcgccgc cttcgccgcg catcacagtg
     6781 cctacgcgcg cgagcgggtc aagcgcggcg tcgcgcgtgc cgtctctccc gcgctcgagc
     6841 gctcgaccta tgtctgggtc tcgagcctgc tgttcattct cgtctgcgcg gcgtggcagc
     6901 cggtcggggg cacgctttac gtccaccggg gactgctcgc gtggctgcac gtgcctgtcg
     6961 ccgcggccgg cgtggccctg acggccatgg gggcgttccg gctgcgcgcc ttcgatctcg
     7021 cggggatcgc ccaggctcgc ggcgaggaag gagaggccgg aaggtcgctc gtcgtggcct
     7081 ggccctacac gatggtccgg cacccgattt acctggcctg gctgcttctg gtcttcagcc
     7141 cgccgtcaat gaccgccacc cggttcgtgt tcgcgtgcgt cagctccgcc tacctggtgc
     7201 tggccgtccc cctcgaggag cgcctgctca tcaagcggtt cggcgaggcc taccgcggct
     7261 accggcggca ggtgaggtgg aggatggtgc cgggggtgta ctgaggcgcc tcactgcagc
     7321 ttcttctcga ttcctccgag cgcttcgagc agcacgtcgc cgacgacctg caggctgcgg
     7381 gcgctgacct tgtcgagggt gtcgcccgcc gtgtgccagg gggggtagtc ctcgggaccg
     7441 tcgatgatgt tcaccgccgg gatgcccgcc cgcaggaagg gcacgtgatc gtcctcgatc
     7501 tgggtggtct ggtcgaggaa cacgtcggtg tgcttcaggc gcctggctgc cgcccagacg
     7561 atgtcggtga gccagcgggt cgatgagagc tcgcggcgga tgtgcaggtc gcggtcgccg
     7621 atcatgtcca ccagcaccat cgcgcgaatc tgcttcagcg acccgtcgcg gcgggcggcc
     7681 tcgacgtagt gccggctgcc gtaggtgttg tcgtggtccg tgtaccagtc gacgaacgcc
     7741 tcctcaccgt cgaagaacac gacctccgag gtgagggcgt tcttgcggtt cttcagcacc
     7801 cgcgcccact cgatcaggaa cgccgtgctc gaccccgcgt cgttggcgcc gacgaaccgg
     7861 aacgcgcgga agagcttcgt gtcgtagtgg ccgccgaaca cgatcttgcc gggcgaccgg
     7921 ccgggtatgg tggcgatcag gttcaccatt cgaaccgggc cgatgggcgt gtcggcggtg
     7981 aaggcctgct cgcgcacctc gacgccggcc gcggccagct gcttcttgat gtagccgcgc
     8041 gccgcctcga gcgccgccga accggccggc cggggcccga agccgacgac ctcgcggacg
     8101 tgggcgtacg ccttcgatcc gtcgaagccg gcggaagctg ccggcgcctg gtggctggag
     8161 gcgcacacgg tccctcccag ggcggcaccg accgcgagcg cggccgccgc catccacgca
     8221 ccgctacgct cagtcttccg gcttctcgtc gtcatcaggg cgctcctcga ccctctcacc
     8281 cgggatgcgg tacttgccgt ccgcccagtc ggccaggtcg gacagcttgc agcgctcgct
     8341 g
//
LOCUS       DAOPQX010000154         8312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9589_length_8312_cov_5.094344, whole genome shotgun sequence.
ACCESSION   DAOPQX010000154 DAOPQX010000000
VERSION     DAOPQX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9589_length_8312_cov_5.094344"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKK95_09455"
     CDS             complement(<1..654)
                     /locus_tag="PKK95_09455"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18483.1"
                     /translation="MADRPELRVSRWPAGRLPARVAVAALVALALGILVSPGLSRRVP
                     HYAAGDYATRSIRAPYDFSVVDVSATERRRRDAAAAAPVVAALDSGAAAKAASRLAGA
                     FAPMARLFEEADAQRVPSESEVRGLSSRARAALEKRRAVEADAWLAGALAKALPAFEA
                     ATGIRLADDERGALERERYSGRVVRLLASVLEAVHAEPIVADRAAFEPLPGAGGGPRP
                     "
     gene            768..2672
                     /gene="speA"
                     /locus_tag="PKK95_09460"
     CDS             768..2672
                     /gene="speA"
                     /locus_tag="PKK95_09460"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOC18484.1"
                     /translation="MQTELRAWTIKDSLELYNVSGWGRDFFSINEAGNVTVTPGGPGS
                     VKIDLKELVDDLRSRGLNLPVLIRFSDILRTRVAQLFGAFQQAIVENEYKGSFRGVYP
                     LKVNQQRQVVEELMQHGRPFNLGVEAGSKPELLVALAVQENPDALILCNGYKDRAFIE
                     TALLAQKLGRRVVITMDRMGELETILAAASELDLRPVIGVRARLATKGAGKWVESTGD
                     RSKFGLTTAEIVETVERLRGVGMLDCLQLLHFHIGSQITNIRAVKDALRESSQIFVEL
                     HRLGANMKYFDCGGGLGVDYDGSQTNFHSSVNYTLGEYAADIVGQIAEVCNARGVPHP
                     DIVTESGRALVAHHSVLVYNVLATNEILGGHPPAAPGPDDHPILQQLWESFSLVSRKN
                     FQETYHDVLQFKEEAITAFNLGVLDLQARARVEQMFWATCEKLLKIVRDLEYVPDELE
                     GLEKQLSDTYYCNFSLFQSMPDHWAVKQLFPTIPIHRLNQEPVRRGILADLTCDSDGK
                     IDEFIDLRDVKGFLELHPPNGGEYLIATFLVGAYQEILGDLHNLFGDTDAVHVKLEGD
                     DYTVQHVVEGDSVTEVLSYVQYNKEDMVARVRRSVEAALREKRLTIAESGRFMKRYEE
                     ALEGYTYLSE"
     gene            2833..4242
                     /locus_tag="PKK95_09465"
     CDS             2833..4242
                     /locus_tag="PKK95_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18485.1"
                     /translation="MSHEFILPEPRIAPQLDPGFRPPALANRAFAREVRESGGGVPLV
                     IAMERSDGAVSRFETRVFSESHPRFQAGLPYAERLFKFLLWQWGGWRAWIGGPRAIGA
                     HIRDTYSPGGARAFDHQFMGEDVYEHTFTVIPCDPAEVPAARESGQPLGRHLDGCRIG
                     FDLGASDRKVSAVIDGQAVYSEEVVWTPSTQSDPEYHYREIRTALETAAAHLPRLDAI
                     GGSSAGVYVNDTVRVASLFRAVPRERYGEVRSLFHRLRQEFGVPLSIVNDGDVTALAG
                     SMSLEDHSVLGIALGSSEAAGYVNAEGNITGWLNELAFAPVDYQPGAPEDEWSGDRGV
                     GARYFSQQCVFRLAPAAGIELPDAVAAEKLKAVQALLESGHEGAESIWRTMGVYLGYT
                     VAHYADFYPLRHVLILGRCTSGRGGTLLLDEARRVLAAEFPEVAASVNIQLPDEKSRR
                     VGQSIAAASLPALDGAEKK"
     gene            4239..5087
                     /locus_tag="PKK95_09470"
     CDS             4239..5087
                     /locus_tag="PKK95_09470"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOC18486.1"
                     /translation="MKPRLETAEIFVPDNLPVDQALARTTHLAVSAHQDDLEIMAFDG
                     VLKAFQQPDKWFTGVVVTDGAGSPRDELYKDYTDEAMRIIRRKEQKKAAYVGDYAAQV
                     LLDYPSSVVKNAADGRPKDDIADLLRATRPEVVYTHNLADKHDTHVATALRVIEAIRS
                     LPEAERPRTLYGCEVWRDLDWMVDADKVGFDLTAHENLQAALLGVFDSQIAGGKRYDL
                     ATQGRRRAHATYYASHGTDTVELLNYAMDLTPLVKDATIDPAAFAEGFIERLRADVLN
                     RVRKFK"
     gene            complement(5155..6396)
                     /locus_tag="PKK95_09475"
     CDS             complement(5155..6396)
                     /locus_tag="PKK95_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HOC18487.1"
                     /translation="MPNLDLNVVDLNVAAVVAIFAGTYLVLALGQTPVFRIDRTGAAL
                     IGAILMVVVGRLSLDEAWRAIDHRTIILLFGMMVLVASLRLGWFFRILGRRAVSSIGH
                     PGALLVAVVFVSGALSALFVNDTICLIFTPLLIEVAAARRLPPLPFLLALATSSNIGS
                     TAAITGNPQNMLIGSLSGISYASFLGALGPVALVGLAIDSAILLALFRRDLRPTPWTA
                     GPEAIRPIHRALTRKGILIAAGVLVAFLSGIDTALVAAVAAAAVLVTRWVKPEKIYRQ
                     VDGGLLVLFMGLFVVIRGVEAAGIDRWLFDLLRPIGVDTVWGLSTVAAVLSNLTSNVP
                     AVMLFSRVVPHLSDAREAWLTLAMASTLAGNLTILGSIANLIVVEGARRHGIRVTFLE
                     YFRVGLPVTLATLAFGVWWLS"
     gene            complement(6445..6945)
                     /locus_tag="PKK95_09480"
     CDS             complement(6445..6945)
                     /locus_tag="PKK95_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC18488.1"
                     /translation="MAKAKKAVPDGYQTVTPSLTLDNAAQAIDWYKRALGAEETERMT
                     GPDGKIMHAELQLGSSRLMVNDAMMGYKGALAMGGSPASFYVYVEDCDSVFNRAVGAG
                     ATVLPGMGALADQFWGDRSGSFTDPFGYRWTIATRKEDLTREEIDQRMTEWMKKFAEQ
                     KKFAHQ"
     gene            complement(7221..7640)
                     /locus_tag="PKK95_09485"
     CDS             complement(7221..7640)
                     /locus_tag="PKK95_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOC18489.1"
                     /translation="MTNDSIREHCLSLEHATEVVQWGDHLLFKVGGKMFAMIDLDGHR
                     CSMRCSPERYAELVEMEDIVPSGHNMWKYHWVTLEALDALPDRELRDALTAAYQLVRA
                     KLPARVRAALDGGASPGRARPKRRGRAGDAKRCRPGR"
     gene            7820..8248
                     /locus_tag="PKK95_09490"
     CDS             7820..8248
                     /locus_tag="PKK95_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18490.1"
                     /translation="MTYEENAIRSSEGGGARLAAGDEMPHFRITTVAGRPFKYGDVWQ
                     RKNVVLITLPDDDAEAARRLPDWTDAAARTSGDDTEWVITSDEVPGVGRPGIVVADKW
                     GEVQFAQSAPTAAGLASGDELAEWLEFVRYQCPECQGEAK"
BASE COUNT         1344 a         2871 c         2909 g         1188 t
ORIGIN      
        1 cggccttggg cccccgccgg cgccgggcag cggttcgaac gccgcgcggt ccgcgacgat
       61 cggctcggcg tgaaccgcct ccagcaccga cgccagcagg cggacgacgc gcccggagta
      121 ccgctcgcgc tcgagcgcgc cgcgctcgtc gtccgccagg cgtatgccag ttgcggcctc
      181 gaaggcggga agcgccttgg cgagcgcgcc ggccagccag gcgtcggcct cgacggcccg
      241 gcgtttctcc agggcggccc gggcgcgcga cgacaggccc cgcacctcgc tctcggaggg
      301 cacgcgttgt gcgtccgcct cctcgaacag ccgggccatc ggcgcaaagg cgccggcaag
      361 gcgcgaggcc gcctttgccg ccgcgcccga gtccagggcc gcgacgacgg gggccgccgc
      421 ggcagcgtcg cggcggcggc gctcggtggc ggacacgtcc acgaccgaga agtcgtaggg
      481 cgcacggatc gagcgggtcg cgtagtcgcc ggcggcgtag tgggggacgc gccgggagag
      541 gccgggcgag acgaggatgc cgagcgcgag ggcgactagc gccgccaccg ccacgcgcgc
      601 gggcagccgg ccagccggcc acctcgatac ccggagctcg ggacgatcgg ccacgctcac
      661 ctccgccggg gaccataccg gccgccaccc gctcgcgtca aggcgaaggg tgctatattc
      721 aatggctcgc gcggcccagc gcggcacgat gaaggggaga aggaaggttg cagactgaac
      781 tgagagcgtg gaccatcaag gactcgctcg agctgtacaa cgtcagcggg tggggccgcg
      841 atttcttctc gatcaatgag gccggcaacg tcaccgtgac gccgggcggc cctgggtcgg
      901 tgaagatcga cctcaaggaa ctggtggacg acctccggag ccgcggcctc aacctcccgg
      961 tcctcatccg gttctccgac atcctgcgca cccgcgtcgc ccagctgttc ggtgcgttcc
     1021 agcaggccat cgtcgagaac gagtacaagg ggagcttccg cggcgtctac ccgctcaagg
     1081 tgaaccagca gcggcaggtg gtcgaggagc tgatgcagca cggccgcccc ttcaacctgg
     1141 gagtcgaggc cggctcgaag cccgagctgc tggtggcgct cgcggtccag gagaaccccg
     1201 acgccctcat cctgtgcaac gggtacaagg accgggcctt catcgagacg gcgctgctcg
     1261 cccagaagct cgggcggcgc gtggtcatca cgatggaccg gatgggcgag ctggagacca
     1321 tcctggccgc cgcctccgag ctcgatctgc ggcccgtgat cggcgtccgg gcgcgactgg
     1381 cgaccaaggg cgccggcaag tgggtcgagt ccacgggcga ccgctcgaag tttgggctga
     1441 ccaccgccga gatcgtcgag acggtggagc ggctgcgcgg ggtcggcatg ctcgactgcc
     1501 tgcagctgct tcacttccac atcgggtcgc agatcaccaa catccgcgcc gtcaaggacg
     1561 ccctgcgcga gtcgagccag atcttcgtcg agctgcaccg cctcggcgcc aacatgaagt
     1621 acttcgactg cggcggcggc ctgggcgtcg actacgacgg cagccagacg aacttccact
     1681 cctcggtgaa ctacacgctc ggggagtacg cggccgacat cgtcggccag atcgccgagg
     1741 tctgcaacgc gcgcggcgtg ccgcaccccg acatcgtgac cgagtcgggg cgggcgctgg
     1801 tggcgcacca ctcggtgctg gtctacaacg tgctggcgac caacgagatt ctcggcgggc
     1861 acccgccggc ggcgccgggt cccgacgacc acccgatcct gcagcagctg tgggagtcgt
     1921 tcagcctcgt ctcgcggaag aacttccagg agacctacca cgacgtcctg cagttcaagg
     1981 aggaggcgat caccgccttc aacctgggcg tgctggacct gcaggcgcgg gcgcgggtcg
     2041 agcagatgtt ctgggccacc tgcgagaagc tgctgaagat cgtccgcgac ctggaatacg
     2101 tgcccgacga gctggagggg ctggagaagc agctgagcga cacctactac tgcaacttct
     2161 ccctgttcca gtcgatgccc gaccactggg cggtgaagca gctgttcccg accatcccga
     2221 tccacaggct caaccaggag ccggtgcgcc gggggatcct cgccgacctg acgtgcgaca
     2281 gcgacggcaa gatcgacgag ttcatcgacc tgcgcgacgt gaagggattt ctcgagctgc
     2341 acccgcccaa cggcggcgaa tacctgatcg cgaccttcct cgtgggcgcc taccaggaga
     2401 tcctcggcga cctccacaac ctgttcggcg acaccgacgc ggtgcacgtg aagctggagg
     2461 gggacgacta caccgtgcag cacgtcgtcg agggcgactc ggtgaccgag gtcctttcct
     2521 acgtgcagta caacaaggag gacatggtgg cccgggtgcg ccgctcggtc gaggcggcgc
     2581 tgcgcgagaa gcggctgacg atcgccgaat cggggcgctt catgaagcgc tacgaggaag
     2641 cgctcgaggg gtacacctac ctgtcagagt aggggcgacc cttgtggtcg tccctacggc
     2701 gacccttgtg gtcgcccgcg ctgcaacggt tcgacgctcg ctagccttct ccattctgcg
     2761 tttttcgtct gcccgcggcc cgacctcctc cgttttccgt tttcccctcc ttgtggatat
     2821 gatcgtggat gcatgagcca cgaattcatc cttcctgagc cccgtatcgc cccccagttg
     2881 gatcccggct tccggccacc cgctctcgcc aaccgcgcct tcgcgcggga ggtgcgcgag
     2941 tcagggggcg gggtgcctct cgtcattgcg atggagcgct ctgacggcgc ggtctccagg
     3001 ttcgagacgc gcgtgttctc cgagtcccac ccgcggttcc aggccggcct gccctacgcg
     3061 gagcggctgt tcaagttcct gctctggcag tggggcgggt ggcgcgcctg gattggcggg
     3121 ccgcgggcaa tcggcgccca cattcgcgac acctattccc cgggcggcgc ccgggcgttc
     3181 gatcaccagt tcatgggcga ggacgtttac gagcacacct tcacggtgat cccgtgcgac
     3241 ccggcggagg tgccggcggc gcgcgagagc gggcagccgc tcgggcgtca cctcgacggg
     3301 tgccggatcg gcttcgacct cggcgcctcc gaccggaagg tgtcggcggt gatcgacggc
     3361 caggccgtct actccgagga ggtcgtgtgg acgccctcca cgcagtccga cccggagtac
     3421 cactaccgcg agatccggac ggccctcgag acggcggcgg cgcacctgcc tcgcctcgac
     3481 gcaatcggcg gcagctccgc cggcgtgtac gtgaacgaca cggtgcgggt ggcctccctg
     3541 ttccgcgcgg tgccgcgcga gcggtacggc gaggtgcggt cgctcttcca ccgcctgcgg
     3601 caggagttcg gggtgccgct gtcgatcgtc aacgacggcg acgtgacggc gctggccggc
     3661 tccatgtcgc tcgaggacca cagcgtgctc ggcatcgcgc tcggctccag cgaggctgcg
     3721 ggctacgtga acgccgaggg gaacatcacc ggctggctga acgagctggc gttcgccccg
     3781 gtcgactacc agcccggcgc gcccgaggac gagtggtcgg gagaccgcgg cgtcggcgcg
     3841 cggtacttct cgcagcagtg cgtgttccgg ctggcgccgg ccgccggcat cgagctgccc
     3901 gacgcggtcg cggccgagaa gctgaaggcg gtgcaggccc tgctcgaatc ggggcacgag
     3961 ggagccgagt cgatctggcg gacgatgggc gtgtacctcg gctacacggt tgcccactac
     4021 gccgacttct acccgctgcg gcacgtcctg atcctcggcc gctgcacctc gggccgcggc
     4081 ggcacgctgc tgctcgacga ggcgcggcgc gtcctggccg ccgagttccc cgaggtggcc
     4141 gccagcgtca acatccagct tcccgacgag aagagccggc gcgtcggcca gtcgattgcg
     4201 gccgccagcc tgccggcgct cgatggagca gagaagaaat gaaaccgaga cttgaaaccg
     4261 cggagatctt cgttcccgac aacctgccgg tcgaccaggc gcttgcccgc accacccacc
     4321 tggcggtgtc ggcacaccag gacgacctcg agatcatggc gttcgacggc gtcctgaagg
     4381 cattccagca gcccgacaag tggttcaccg gcgtggtcgt caccgacggc gccggctcgc
     4441 cgcgcgacga actgtacaag gactacaccg acgaggcgat gcggatcatc cggcgcaagg
     4501 agcagaagaa ggcggcctac gtcggcgact acgcggcgca ggtgctgctc gactacccga
     4561 gcagcgtggt caagaacgcc gccgacggcc ggccgaagga cgacattgcc gacctgctcc
     4621 gcgcgacccg ccccgaggtg gtgtacacgc acaacctcgc cgacaagcac gacacccacg
     4681 tggcgacggc cctgcgcgtg atcgaggcga ttcgcagcct gcccgaggcc gagcgcccca
     4741 ggacgctcta cggctgcgag gtgtggcgcg atctcgactg gatggtggac gccgacaagg
     4801 tggggttcga cctgacggcg cacgaaaacc tgcaggcggc gctgctcggg gtcttcgact
     4861 cgcagatcgc cggcgggaag cgctacgacc tggccaccca ggggcggcgc cgggcgcacg
     4921 cgacctacta cgcctcgcac ggcaccgaca cggtcgagct gctcaactac gcgatggacc
     4981 tgacgccgct cgtcaaggac gcgacgatcg atcccgcggc cttcgccgag ggcttcatcg
     5041 agcgcctgcg ggccgacgtc ctcaaccggg tccgcaagtt caagtagggc aacccttcag
     5101 ggttgccgaa ccgcaaccag ccaatggggc agggcttcgg tcctgccccg ccctctacga
     5161 cagccaccac acgccaaagg cgagcgtcgc cagcgtcacc ggcagcccca cgcggaagta
     5221 ctcgaggaac gtcacgcgga tgccgtgccg cctggcccct tcgacgacga tcaggttcgc
     5281 aatcgagccg aggatcgtga ggttgcccgc cagcgtgctc gccatggcca gcgtcagcca
     5341 ggcctcccgg gcgtccgaca ggtgcggcac gacccggctg aagagcatca ccgccgggac
     5401 gttgctcgtc aggttcgaga ggaccgcggc gacggtgctc agcccccaca cggtgtccac
     5461 cccgatcggc cggagcagat cgaagagcca gcggtcgatc ccggccgcct cgacgccgcg
     5521 gatcacgacg aacaggccca tgaacagcac gagcaggccg ccgtccacct gccggtagat
     5581 cttctccggc ttcacccagc gggtgaccag caccgccgcg gcggccaccg cggccaccag
     5641 cgccgtgtcg atgcccgaga ggaaggcgac cagcaccccg gccgcgatga gaattccctt
     5701 gcgggtcagc gcccggtgga tggggcggat ggcctccggg ccggccgtcc agggcgtggg
     5761 ccggaggtcg cggcggaaca gcgccagcag gatggccgag tcgatcgcca ggcccacgag
     5821 ggcgaccggg ccgagcgcgc ccaggaagga cgcgtacgaa atccccgaca ggctcccgat
     5881 cagcatgttc tgggggttgc cggtgatggc ggccgtgctg ccgatgttcg aggaggtggc
     5941 cagggcgagc aggaacggga ggggaggcag gcgccgcgcc gcggcaacct cgatcagcag
     6001 cggcgtgaag atcaggcaga tggtgtcgtt gacgaacagg gccgagaggg cgccgctgac
     6061 gaacaccacg gccaccagca gcgcgccggg atgcccgatg ctcgagaccg cgcggcgccc
     6121 caggatgcga aagaaccatc ccaggcgcag gctggcgacc agcaccatca tcccgaacag
     6181 caggatgatc gtccggtggt cgatcgcccg ccacgcctcg tccagcgaga gccgaccgac
     6241 caccaccatc aggatcgccc cgatgagcgc ggcgccggtg cgatcgatgc ggaagacggg
     6301 cgtctgcccc agtgcgagca ccaggtaggt gcccgcgaag atcgcgacga ctgccgcgac
     6361 gttgagatcg acgacgttca ggtcgaggtt gggcatgcgg gcggggggcg gcctccatca
     6421 tcgcacgcgc cgccccctgc cgtcctactg gtgggcgaac ttcttctgtt cggcgaactt
     6481 cttcatccac tcggtcatcc gctggtcgat ctcctcgcgc gtgaggtcct ccttgcgggt
     6541 ggctatcgtc cagcggtagc cgaacgggtc ggtgaagctg ccggagcggt cgccccagaa
     6601 ctggtcggcc agcgcgccca tgccgggcag gaccgtggct cccgcgccga cggcgcggtt
     6661 gaacacggag tcgcagtcct cgacgtagac gtagaacgag gcgggggagc cgcccatcgc
     6721 cagcgcgccc ttgtacccca tcatcgcgtc gttcaccatg aggcgcgaac ttccgagctg
     6781 cagctcggcg tgcatgatct tgccgtcggg tcccgtcatc cgctcggtct cctcagcgcc
     6841 taacgcgcgc ttgtaccaat cgatcgcctg cgcggcgttg tccagcgtga gggacggggt
     6901 gacggtctgg tagccatcgg gaacggcctt cttcgctttt gccatctcga cctcccggcc
     6961 ccggcgagcg gggccgattt ccacaggggc gttccgccgc cggcccacga gccggctcct
     7021 cgaggacgcc agttgaaggt agttagccgc cgcctttcgt gcaagccgac ggccggatgg
     7081 caaaggacgg gggcggcagc cgcaaaccgc tgcgtgacag cctgttcaga ggacggctcg
     7141 ggcacgggca tgcagaggtc ccgtgtagcg ggaggcgaca tcggcggcgc ggttgcgtag
     7201 cgggacccgc gccggtcgcc tcaacggccc ggccggcacc gcttcgcgtc gccagcccgt
     7261 ccccgacgct tcggccgggc gcggccgggt gaggcgcccc catcgagcgc ggcccggacc
     7321 ctggcaggca gtttcgcacg cacgagctgg tacgcggcgg tgagcgcgtc ccgcaactcg
     7381 cgatcgggca gcgcgtccag cgcctcgagc gtgacccagt ggtacttcca catgttgtgg
     7441 ccggagggga cgatgtcctc catctcaacc agctcggcgt aacgctcggg ggagcacctc
     7501 atgctgcagc gatggccgtc caggtcgatc atcgcgaaca tctttccccc gaccttgaag
     7561 agcaggtggt ctccccactg gaccacctcg gttgcgtgct cgagggagag gcagtgctcg
     7621 cgaatggagt cgttggtcat ggttcccagt ggcacaggcg tcccgcctgt gagagccgtc
     7681 gtatggtctt acgacgcgcg ctgcggccta tcttgcacca tacacaggcg agatgcctgt
     7741 gccactctgc cacaggcgag accctctgcc agggtgccac aggcgagacg cctgtgccac
     7801 tgtgccactg gagcgcgaga tgacgtacga agagaacgca atcaggtcga gtgagggagg
     7861 gggcgcgcgg ctggcggcgg gcgacgagat gccgcacttc cggatcacga cggttgcggg
     7921 ccggcccttc aagtacggcg acgtctggca gcggaagaac gtggtgctga tcaccctgcc
     7981 ggacgacgac gccgaggccg cccggcgcct ccccgactgg accgacgcag ccgcccggac
     8041 ctctggcgac gacaccgagt gggtcatcac atcggacgaa gtgcccggcg tcggaaggcc
     8101 gggcattgtc gtggcggaca agtggggcga ggtccagttc gcgcagtcgg cgcccacggc
     8161 ggcaggcctc gccagcggcg acgagttggc ggagtggctc gagttcgtcc gctaccagtg
     8221 ccccgagtgc cagggcgagg cgaagtagcg ccgggtcgcg ttccgcctgc tggggcgcgg
     8281 cggctgcacg agagcgccgg ctgacggtca gt
//
LOCUS       DAOPQX010000155         8222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9733_length_8222_cov_5.686788, whole genome shotgun sequence.
ACCESSION   DAOPQX010000155 DAOPQX010000000
VERSION     DAOPQX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9733_length_8222_cov_5.686788"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKK95_09495"
     CDS             complement(<1..458)
                     /locus_tag="PKK95_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18491.1"
                     /translation="MPDANQDRSIGPALLGWLEAPRHGERVGRFVHLAGWVFAREGQV
                     LSLTAAVGRARHAVPHGLPRPDVAEAYGNAAGARYSGFDTYLPVEPGVAGTRLTVWAA
                     LPNGRRVPCFARCVIGTPKLDQPWWRALADKVGLGSPVGPATADALGPAGP"
     gene            745..1389
                     /locus_tag="PKK95_09500"
     CDS             745..1389
                     /locus_tag="PKK95_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOC18492.1"
                     /translation="MRRGTADTVVFEEVFFQGQCTFGIECEGAGLIVDAGAHVGLTTV
                     FFALQYPRATIVALEPERSNFHLLRANTAAFRNVRAVNAALWHHPAKLCLANPGSDTW
                     TFRFVPPGPTAGAGSEAGATFVEAITMDALLAPYGTRRVSLLKMNIEGAEAEVLRASA
                     SWLHRVDSLAVELHDWLRPGCADALDQACPPGRYLRSRSGEFTVLTRAPDQGRP"
     gene            complement(1358..2113)
                     /locus_tag="PKK95_09505"
     CDS             complement(1358..2113)
                     /locus_tag="PKK95_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC18493.1"
                     /translation="MAVIEARALAKSFRIPSDRRETVREHLFNAFRPRSFERLDVLRS
                     VSFDVRKGETLGIMGRNGSGKSTLLKILCGIYRADRGDLLVGAPLTPILELGLGWNPE
                     LDGIDNILLLGTAMGLRLREAKNAVDEILAFAGLDRFANVPLKHYSSGMASRLAYSVA
                     FKTVREVLVLDEIFAVGDAEFRERCEARYRELSAKGHTVVLVSHDPRVISAFCSRALL
                     LDEGRVAFEGRGGEVVDEYSRRVAMASPGPALA"
     gene            complement(2113..2898)
                     /locus_tag="PKK95_09510"
     CDS             complement(2113..2898)
                     /locus_tag="PKK95_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18494.1"
                     /translation="MRGSVTAAGTRVDVAGLLWTLARTDFKTRYHGTFGGFLWALLKP
                     ALMFVVLMSVFSFIFATSPNYRLNLIVGLFLWDFFAEATKTGLTSLHAKGYLLAKTRF
                     PSWILVVASASNALLTLAVFLAIFLAYLAFAGRAPGAAAAALTLFYLVLFFAITVGIS
                     LAGSVLFLRYRDLNQVWDVAVQAGFFVAPIIYPLEILPERFHFWLYLWPPTPVIQFCR
                     AVLVDGVLPTARANLLLAAMAAVILAVGVLVFSSQVADAAERL"
     gene            complement(2900..3982)
                     /locus_tag="PKK95_09515"
     CDS             complement(2900..3982)
                     /locus_tag="PKK95_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC18495.1"
                     /translation="MTSPPRIIALVSAYNEADIIAASVDHLSGQGIGVYLLDDGSTDE
                     TLSIARARMGRGIVGVEELPSPGPAGMPRRFSLSRILERKEELARTLDAEWFVNQDAD
                     EFRESPWPHLTLAAAIGLVDRLGWNAIDFEVLNFVPGEEEYEPGSDLARVFRRCVPGG
                     AFDRLQVRCWKKPAGGVDLVSSGGHDVGFEGRRVFPIRFPMRHYPIRGEAHGRRKVFQ
                     ERLDRFDPAERSAGWHVQYDGVVPGGPLTAGWGPAVEYDRDALAVGLQLRHRLVERLI
                     EAGGRLASQRWRLEEETARLAEALRRSEERAATAEASARRAEGLAAGLAAGAEELERR
                     LGEVFASKSWRVTAPLRAVWRWLGGR"
     gene            complement(3993..5720)
                     /locus_tag="PKK95_09520"
     CDS             complement(3993..5720)
                     /locus_tag="PKK95_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC18496.1"
                     /translation="MRQKRWLPIVLCAGAATLAVVVYLNALDNPFVWDDRRTVVENAS
                     IRPPLDLGALVHQDLFRPLVNFTYAANYAVGGLSVTGYHLFSVLLHALNVVLLFVFTR
                     RTFAAWRVKRVAQASSPVRAGSANTAAFAAASIFAVHPLMTQAVGYVSGRAEVLAAVF
                     FLTACLAAQAWVRREGLAALVLMGVAWLFALLSKEVAIALPAVVAAYAIFASDLDGAG
                     RRRLAYLVAVMLGLMAGVGGYRLMTLVTVENAGQSQVIGGNVLVGLDMVRQYTRLMAI
                     PAGQSIFHSSSYAEVTSPGKVVLNLLWLAGLCLLAWRSRRWEPVAGFGIAWFLVLLLP
                     SSFLLLLDIGEPMAEQRVYLAACGFTMACGAVIGRLAAWPRTRRLTLAGLAAALVPLA
                     VLTVERNEVWGDPVALWSEAVAREPRVWVPYVGLGDVLRERGDYAAAAEAYRDAARLH
                     PESRTFLPLATCLLMTGRVEEAGEYFARADRLSPGGVDAPVGLALVARLEGRRDEARD
                     RFEAVVRAHPEAVLPRRFLAEMYEREYADPESALRVCREIQAMAPATPGVDECVRRNE
                     PGAQNGNGR"
     gene            5751..8162
                     /locus_tag="PKK95_09525"
     CDS             5751..8162
                     /locus_tag="PKK95_09525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC18497.1"
                     /translation="MPPLRILHVAPYSERAWAYGGIPRVLASLAHAQARGGHRVTIAT
                     TDVRDATSRLAPPGAARPPRLRLRSWNERLDDGIEAFVFPNLSNRIANRWQFYQPLGL
                     GGFLRRHAGDFDVAHLHACHNLATALAARVLAGAGVPYVVQPNGTARRIERRRAAKWL
                     FDSLFARRLLEGAAAVIAVSEWERRQLEAGLVAPDRCHLVPNPLAPAPEPLPGGAAFR
                     RRLGLSADPLILFLGVLSPRKHPEVLVEAASSLRRPVQLLFAGADGGALAATRQAAER
                     LGLLDRVRFAGVLEGAARFAALASADVAVYASHDEAFGLSALEALQAGTPVVVGDDAG
                     CAEVIASVEGGLLATPGDASALAVALDRMLARLPVWKAVARQAGAIASKRFHPDAVWS
                     SLEPVYRSVSAEPGVLALATSVPAPVEDPAPAAAEVPAPGDIEVPAPVAVEVPAAVPE
                     PERAETPVRPHSGVTFIVAVHNGERWLEQVLEAIDAQRDGRPFEVVAVDDGSTDSSPA
                     VLEKWSQRKGIRVIRLEDGGHPAAVNAGVRAASHPIVCLVDQDVVLQPGWLQAIGAAL
                     DEPGVAAVQGTFAIEQGASLVARVMGLDLGQRYTAMAGPATSHVCTGNSAYRVDALRQ
                     VGLFFDESFGYGADNDMSYRLAGAGYELRFCAEAKAVHHWRESLWSYLRQQYGFGYGR
                     LDLVAKHPRRAGGDSVSPAAMMAHGPLMLAALGLALVWLASLAWGRMVALAGWLALVI
                     VAGLAIERLAAGIRAASRHKDAAGLWFAPFHIARDVAWAFAIVAWAFRRLLGRTPRPE
                     HSMRR"
BASE COUNT         1244 a         2957 c         2911 g         1110 t
ORIGIN      
        1 gggcccgctg gcccaagcgc atccgccgtc gccgggccga cagggctgcc gagtccgacc
       61 ttgtcggcca gcgcgcgcca ccagggctgg tccagcttcg gcgtgccgat cacgcaccgg
      121 gcgaaacacg ggacacgtct gccattgggg agtgccgccc agacggtgag cctggtgccg
      181 gccacccccg gttcgacggg aaggtacgta tcgaaccccg aataccgcgc cccggcggca
      241 ttgccgtatg cctccgcgac gtccggccgc ggaagcccgt ggggaacggc gtgccgcgct
      301 cgtcccacgg cggctgtcag cgagaggacc tgcccctcgc gggcaaagac ccatccagcc
      361 agatgcacga accggccgac gcgctcgccg tgacgcggcg cctccaacca gcccagcagg
      421 gccggtccga tggagcgatc ctggtttgcg tcaggcatgt ccgcagtgcc gcgacatcgt
      481 agcccgaaca ttcgggaggg cccagaacaa tccgcgcttc gccctgctgt agactggccc
      541 gaccgcgaag cctgcggccg gaggactcgg cacgtgcaga caaggtcgct ccgaagattc
      601 gtttctcagc aggaccgcga gttcctgcgc tgggccatcc ggcgattcgg cgttcttcgc
      661 gccggacgcg agtaccttcg ttctctgggc aagccaaccc atctcgtccg gctccctgtt
      721 ccgggcgcgc ggcacgaggt tctcgtgcgg cgggggacgg cggacacggt ggtgttcgag
      781 gaggtgttct tccaggggca gtgcacgttc ggcatcgaat gcgaaggggc cggcttgatc
      841 gtggatgcgg gcgcccacgt cgggctgacc acggtcttct tcgcactcca gtatccccgc
      901 gccacgatcg tcgccctcga acccgagcgc tcgaacttcc acctccttcg agcgaacacc
      961 gcggcgttcc ggaacgtcag ggccgtcaac gccgccctgt ggcaccatcc cgcgaagctc
     1021 tgcctcgcca accccgggtc cgacacctgg acgtttcgct tcgtgcctcc cggcccaacc
     1081 gccggtgcag gcagtgaagc aggcgcgacc ttcgtcgagg ccatcaccat ggacgcactg
     1141 ctcgctccct atggaacgcg gcgcgtgagc ctgctgaaga tgaacatcga gggcgcggag
     1201 gcggaggtgc tgcgggcctc ggcgagctgg cttcatcggg ttgacagcct ggcggtggag
     1261 cttcacgact ggcttcggcc gggctgcgcc gacgccctcg accaggcctg ccctccgggg
     1321 aggtacttgc ggtcgcggtc gggcgagttc accgtgctca cgcgagcgcc ggaccagggg
     1381 aggccatagc cacgcggcgg ctgtactcgt ccacgacctc gccgccgcgg ccctcgaacg
     1441 cgacgcgtcc ctcgtcgagc agcagggcgc ggctgcagaa ggccgagatc acccgcgggt
     1501 cgtgggacac gagcacgacg gtgtggccct tcgcgctcag ctcgcggtac cgcgcctcgc
     1561 accgctcgcg gaactcggcg tccccgaccg cgaagatctc gtcgagcacg aggacctcgc
     1621 gcacggtctt gaaggcgacc gagtaggcca gcctcgacgc catgccgctc gagtagtgct
     1681 tcagcgggac gttggcgaag cggtcgagcc ccgcgaaggc gaggatctcg tccaccgcgt
     1741 tcttcgcctc gcgaaggcgg agccccatcg cggtgccgag gagcaggatg ttgtcgatgc
     1801 cgtccagctc ggggttccag ccgaggccca gctcgaggat cggggtcagc ggggcgccga
     1861 cgaggaggtc gccccgatcc gcccggtaga tgccgcacag gatcttgagc agcgtgctct
     1921 tgcccgagcc gttgcggccc atgattccca gcgtctcgcc cttccgcacg tcgaagctca
     1981 ccgagcgcag gacgtcgagg cgctcgaagg atcgcggacg gaacgcgttg aacaggtgct
     2041 cgcgcaccgt ctcgcgccga tcgctcggga tccggaacga cttcgcgagg gctcgtgcct
     2101 cgatgaccgc cactagagcc tctccgcggc gtcggcaacc tggctcgaga acaccaggac
     2161 cccgaccgcc aggatgaccg ccgccatcgc cgcgagcagc aggttcgccc tcgcggtggg
     2221 aagcacgccg tccaccagca cggcgcggca gaactggatt accggggtcg gcggccacag
     2281 gtagagccag aagtggaagc gctcggggag gatctcgagc gggtagatga tcggggccac
     2341 gaagaacccc gcctggaccg ccacgtccca gacctggttg aggtcgcggt agcgcaggaa
     2401 cagcacgctt ccggcaagtg agatcccgac ggtgatcgcg aagaacaaca cgaggtagaa
     2461 cagggtgagg gcggccgccg cggcgccagg ggcccgcccc gcgaacgcca ggtaggcgag
     2521 gaagatcgcc aggaagacgg cgagcgtcaa tagcgcgttc gaggccgaag cgacgaccag
     2581 gatccacgaa gggaaccgcg tcttggccag caggtagccc ttcgcgtgca gcgaggtgag
     2641 gccggtcttg gtggcttcgg caaagaagtc ccagaggaag aggccgacga tgaggttgag
     2701 ccggtagttc gggctggtgg cgaagatgaa cgagaagacc gacatcagga ccacgaacat
     2761 gagcgcgggc ttcagcagcg cccagaggaa gccgccgaag gtcccgtgat accgggtctt
     2821 gaagtcggtg cgggcgagcg tccagagcaa gcccgccacg tccacgcgcg tgccggctgc
     2881 cgtgacgctc cccctcatct caacgtcccc caagccatcg ccagaccgcg cgcagcgggg
     2941 cggttacccg ccagctcttc gacgcgaaca cctcgccgag gcgccgctcg agttcctcgg
     3001 cgccggccgc cagcccggcc gccagccctt ccgcccgccg tgcagacgcc tcggcggtcg
     3061 ctgcccgctc ctcgcttctg cgcaacgctt cggcgagccg ggccgtctcc tcctccagcc
     3121 gccagcgctg gctcgccagc cgaccgcccg cctcaatcag ccgctcgacg agccggtgcc
     3181 gcaactgaag gccgaccgcg agcgcgtccc gatcgtattc gaccgccggc ccccagcctg
     3241 cggtcagcgg cccgcccggc acgacaccgt cgtactgcac gtgccacccc gccgagcgct
     3301 ccgccgggtc gaaccggtcg agccgctcct ggaacacctt ccggcggccg tgcgcctcgc
     3361 cgcggatggg gtagtggcgc atggggaaac gaatcgggaa gacacggcgg ccttcgaacc
     3421 cgacgtcgtg acccccgctc gacaccaggt ccacgccgcc ggccggcttc ttccagcacc
     3481 gcacctgcaa ccggtcgaaa gcgccccccg ggacgcagcg gcggaagacc cgggcgagat
     3541 ccgagccggg ttcgtactcc tcctccccgg gcacaaagtt caggacctcg aagtcgatcg
     3601 cgttccaccc gaggcgatcg acgagcccga tggcggcagc gagggtcagg tgtggccagg
     3661 ggctctcgcg gaactcgtcg gcgtcctggt tgacgaacca ctccgcgtcc agggtgcgag
     3721 ccagctcctc cttcctctcg aggatcctcg acaggctgaa tcgccgcggc atcccagccg
     3781 ggccgggcga gggcagttct tcgacgccca cgattccccg gcccatccgg gcgcgcgcaa
     3841 tcgagagcgt ctcgtcggtc gagccgtcgt cgagcaggta gaccccgatg ccctgcccgg
     3901 agaggtgatc gacggaagcg gcgatgatgt ccgcctcgtt gtaggcggac accagggcga
     3961 tgatccgagg gggtgatgtc atctgggccc gcctaccgcc cgttgccgtt ctgcgcgccc
     4021 ggttcgtttc gcctcacgca ctcgtcgacg cccggcgtcg ccggcgccat cgcctggatc
     4081 tcgcggcaga cgcgcagcgc cgattccggg tcggcgtact cgcgctcgta catctcggcg
     4141 aggaagcggc gaggcagcac cgcctcgggg tgtgcgcgca cgaccgcctc gaagcggtcg
     4201 cgcgcctcgt cgcgacggcc ctcgaggcgg gcgaccagcg cgagacccac cggcgcgtcc
     4261 acgccgcccg gcgacaggcg atcggcgcgc gcgaagtact cgccggcctc ctcgacgcgt
     4321 cccgtcatca ggaggcaggt cgcgagcggc aggaacgtgc gcgactccgg gtgcaggcgc
     4381 gctgcgtcgc gataggcctc ggcggccgcc gcgtagtcgc cgcgctcgcg caggacgtcg
     4441 ccgaggccga cgtacggcac ccacacgcgc ggctcgcggg cgacggcctc cgaccacaga
     4501 gcgaccgggt cgccccacac ctcgttgcgc tccaccgtga ggaccgcgag cggcacgagc
     4561 gcggccgcga gccccgcgag cgtgaggcgg cgcgtgcgag gccaggcggc caggcggccg
     4621 atgacggcgc cgcacgccat cgtgaacccg cacgccgcga ggtagacgcg ctgctcggcc
     4681 atcggctcgc cgatgtcgag cagcagcagg aacgaggacg ggagcagcag gacgaggaac
     4741 caggcaatcc cgaagcccgc caccggctcc cagcgccgcg accgccaggc cagcaggcac
     4801 agccccgcca gccacagcag gttgagcacg accttccccg gggaggtcac ctccgcgtag
     4861 ctgctcgagt ggaagatcga ctggccggcg gggatcgcca tcaggcgcgt gtactgccgc
     4921 accatgtcga ggcccacgag cacgttgccg ccgatgacct gggattggcc ggcgttctcg
     4981 accgtcacca gcgtcatgag gcggtagccg ccaacgcccg ccatcagccc cagcatcacc
     5041 gcgacgaggt aagcgagccg ccggcgcccg gcaccgtcga ggtcggaagc gaagatcgcg
     5101 tacgccgcca cgaccgccgg gagcgcgatc gccacctcct tcgagagcag cgcgaagagc
     5161 cacgcgacgc ccatcaggac gagcgccgcc aggccctcgc gcctcaccca tgcctgcgca
     5221 gccaggcagg ccgtgaggaa gaagaccgcg gcgaggacct cggcgcgccc gctgacgtac
     5281 cccacggcct gcgtcatcag cgggtggacg gcaaagatcg acgccgcggc gaaggcggcg
     5341 gtgttggcgc tgccagctct cacgggcgag gacgcctgtg ccactcgctt cacccgccag
     5401 gcggcgaagg tgcgcctcgt gaacacgaac agcagcacga cgttcagcgc gtggaggaga
     5461 acgctgaaca ggtggtaacc ggtgaccgac aggccgccca cggcgtagtt ggcggcgtag
     5521 gtgaagttga ccagcggccg gaagaggtcc tggtggacga gggcgcccag gtcgagcggg
     5581 ggccgaatcg acgcgttctc gaccaccgtc cgccggtcgt cccagacgaa cgggttgtcg
     5641 agcgcgttga ggtagacgac gaccgccagg gtcgcggccc cggcgcagag cacgatcgga
     5701 agccagcgtt tctgtcgcat ctcggccggg gcgcgcttag tataacggcg atgccgccgc
     5761 tgaggatcct gcacgtcgcc ccctactccg agcgcgcatg ggcgtacggc ggcattcccc
     5821 gcgtgctcgc ctcgctcgcg cacgcgcagg ctcgcggcgg ccatcgcgtc acgatcgcca
     5881 cgaccgacgt gcgcgacgcg acgtctcgcc tggcgccgcc cggcgccgcc cgcccgccgc
     5941 gcctgcgcct gcgttcctgg aacgagcggc tcgacgacgg gatcgaggcg ttcgtctttc
     6001 cgaacctctc caaccggatc gccaaccggt ggcagttcta ccagccgctg gggctcggag
     6061 gcttcctgcg ccgccacgcg ggcgacttcg acgtggctca cctccacgcg tgccacaacc
     6121 tggcaactgc cctcgcggcc cgcgtgctcg ccggcgcggg cgtgccctac gtcgtgcagc
     6181 cgaacgggac ggcgcggcgg atcgagcggc gacgcgcggc caagtggctg ttcgacagcc
     6241 tgttcgcccg ccgcctgctc gaaggggcgg cagcagtcat cgcggtgtcg gaatgggagc
     6301 ggcggcagct cgaggccggc ctcgtcgcgc ccgaccgctg ccacctggtg cccaacccgc
     6361 tggccccggc gccggagccc ctgccgggcg gggccgcctt ccgccggcgt ctcggcctct
     6421 cggccgaccc cctcatcctg tttctcggcg tcctgagccc ccgaaagcac cccgaggtgc
     6481 tggtcgaggc ggcctcgtcg ctccgccgcc cggttcaact cctgttcgcc ggcgccgacg
     6541 ggggagcgtt ggccgccacc cgccaggcgg ccgagcggct ggggctgctc gaccgcgtcc
     6601 ggttcgccgg cgtcctcgag ggtgccgcgc gcttcgccgc gctcgcgtct gccgacgtgg
     6661 cggtctacgc ctcgcacgac gaggcgttcg gcctgtcggc cctcgaggcg ctgcaggccg
     6721 ggacgcccgt ggtcgtgggc gacgacgccg gctgcgccga ggtgatcgcg tcggtggaag
     6781 gggggctgct ggcgacgccg ggcgacgcga gcgccctcgc cgtggctctc gaccgcatgc
     6841 tcgccaggct tccggtgtgg aaggcggttg cccgccaggc gggggcgatc gcatcgaagc
     6901 gctttcaccc ggacgcggtc tggtcgagcc tcgagccggt ctaccgatcg gtgtccgccg
     6961 agcccggcgt acttgcccta gcgacgagcg ttcctgcccc agtcgaggat cctgcccctg
     7021 cggcggcaga ggttcccgcc ccgggggaca tcgaggttcc tgcgcccgtg gcggtcgagg
     7081 ttcccgcggc cgtgccggag cccgaacggg ccgagacgcc ggttcgccca cactccgggg
     7141 tgaccttcat cgtggccgtg cacaacggcg agcggtggct cgagcaggtg ctcgaggcga
     7201 tcgacgccca gcgcgacggc cggcccttcg aggtggtcgc cgtggatgac gggagcaccg
     7261 actcgtcgcc ggcggtcctc gagaagtggt cgcagcgaaa aggcatccgg gtgatccggc
     7321 tggaagacgg aggccacccg gcagcggtga atgccggcgt gagggccgca agccacccga
     7381 tcgtctgcct cgtggaccag gacgtggtgc tgcagccggg gtggctgcag gcaattgggg
     7441 ccgcgctcga cgagcctggc gtggcggcgg tgcaggggac attcgccatc gagcagggcg
     7501 cgtcgctggt ggcgcgggtg atggggctcg acctggggca gcgctacacg gcgatggcgg
     7561 gcccggcgac gagccacgtc tgcaccggga actcggcgta ccgggtggac gcgctgcgcc
     7621 aggtggggct cttcttcgac gagtcgttcg ggtacggggc cgacaacgac atgagctacc
     7681 gcctcgccgg cgccggctac gagcttcgct tctgcgcgga ggcgaaggcg gttcaccatt
     7741 ggagggagtc tctctggagc tacctgcggc agcagtacgg cttcggctac gggaggctcg
     7801 acctggtggc caagcacccg cgccgtgccg gcggcgacag cgtctcgccg gccgcgatga
     7861 tggcgcacgg gccgctgatg ctcgcggcgc tcggcctcgc cctcgtctgg ctggcctcgc
     7921 tcgcctgggg gcggatggtc gcgctcgcgg gatggctggc gctggtcatc gtcgcggggc
     7981 ttgcgatcga gcggctcgcg gcggggatcc gagcggcctc caggcacaag gacgccgccg
     8041 gtctctggtt cgcgccgttc cacatcgcgc gcgacgtcgc ctgggctttc gcgatcgtgg
     8101 cctgggcatt ccggcggctg ctcggcagga ccccacgccc cgaacacagc atgaggcggt
     8161 agctgacagc tgacagcaga cagcagacag ctgacagctc acagctgaca gcggagagca
     8221 ga
//
LOCUS       DAOPQX010000156         8209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9751_length_8209_cov_5.156856, whole genome shotgun sequence.
ACCESSION   DAOPQX010000156 DAOPQX010000000
VERSION     DAOPQX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9751_length_8209_cov_5.156856"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1151
                     /locus_tag="PKK95_09530"
     CDS             156..1151
                     /locus_tag="PKK95_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOC18498.1"
                     /translation="MLERIRVYARAVARFLEARSPEERRKRANHAFALLLVAGVAVIK
                     HVTGLTDERAPFTLYVLAVAAAAARGGFESGLVATLASVLAAGFGAALLGPSRLLFAA
                     EGLLAALVVSTVRSSVRMREAQLRGAEETIADLRARDRHAQRLDAARRQEWEEYREAV
                     ARAQAALQEAADGARRQLGALESLTDPSLNPLGGDDMVSELLARLRGAVGADAAALVQ
                     PGTQGTAVVTVGALEPAAGPIDTAGQRLAQGRVAVVHNDPARVAQLSALRWPDSVTSL
                     LVVPVLHDGQVWSTIEVVSERSRQVSDWDVALARVAADRLAAVVVQGRALASRAS"
     gene            complement(1179..1493)
                     /gene="sugE"
                     /locus_tag="PKK95_09535"
     CDS             complement(1179..1493)
                     /gene="sugE"
                     /locus_tag="PKK95_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HOC18499.1"
                     /translation="MAWLILLVAALFEVGWAVGLKYTNGFTRLWPTAWTLAALAASMA
                     LLAAAARTLPIGTAYAVWTGIGAAGTALLGIWLFQEPASAARLSCIALIVAGVLGLKL
                     AS"
     gene            complement(1514..2941)
                     /locus_tag="PKK95_09540"
     CDS             complement(1514..2941)
                     /locus_tag="PKK95_09540"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOC18500.1"
                     /translation="MRATTVQGIDIDLEPAAFDRLKANLRGLLLLPADPGYEASRTLW
                     NAMIDRKPAAIACCRGASDVVACVRFAREHDILLCIKGGGHNIAGLAAADGALMLDMS
                     LMRGVWTDPRGQVARAQAGCLLGDVDRETQLHGLAAVLGFVSLTGVAGLTLGGGFGYL
                     TRRWGWTADNVKGFELVTAESRIVRADARENPDLFWALRGGGGNFGVVTGIEYSLYPV
                     GPEVVGGLVAWPASEASRVLELYRTLAEQAPRELTLVALMRPAPPAPWLPPEMHGKPI
                     VALLACHSGNPEEGERAVAPIKAFGRPVGDTLVRRPYVQIQALLDATQPKGRRYYWKS
                     EYLPRIEPALCDKVAEHAARVRSPHSAVILFQIGGALNDLAEDHSPAANRDARYVLNV
                     TASWEQPADDGDNAGWARTAWGDMKRFSTGGNYINFLTADEGPDRVEAALGASLGRLA
                     EIKAAWDPQNVFRTNRNIEPASRRP"
     gene            3055..3675
                     /locus_tag="PKK95_09545"
     CDS             3055..3675
                     /locus_tag="PKK95_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18501.1"
                     /translation="MEQHVAAEHLQVIRTLMERSALYRRTLAPIMLYAGSVGCVASVG
                     GILLGIDALPAFSIWWLIAALVAIGGSFAIARRQAVEDGEPFWSPPALRVTQALAPPL
                     ASGLLLSLGLIAFGGDDLRWVFVLANALFYGCAVHAAGFFMPRGMKLFGWLIIAAACA
                     GVLGRPALGGVATSRPDHALMGGLFGLAHLGYGVYLYATEKRKTSS"
     gene            3672..3980
                     /locus_tag="PKK95_09550"
     CDS             3672..3980
                     /locus_tag="PKK95_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOC18502.1"
                     /translation="MNPEALLQLDRVIHEKGRLAIMSMLAASPELSFTELRETLQMTD
                     GNLTTHIRTLQEAGYVAVAKSYRNKRRLTTVSMTEAGRAAFAAYLALLERIVREAKPR
                     "
     gene            4164..5027
                     /gene="ispH"
                     /locus_tag="PKK95_09555"
     CDS             4164..5027
                     /gene="ispH"
                     /locus_tag="PKK95_09555"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOC18503.1"
                     /translation="MRASHLGMCFGVRGAIARVFRIAHREPVTVLGELVHNAAVLDRL
                     AASGVLFQREAEAVETPVVAITAHGASRRRLAHVRSLGLKVVDATCPLVRTAHQAVSQ
                     FVEEGFHPVIVGVRDHVEVRGLTGDLDAYDVVASEADVARVAPRPRFGVAAQTTQPIE
                     RVRHLASVLQARFPGSEVRLADTVCLPTKLRQEAAVALSTECDVVIVVGGANSNNTRE
                     LCETCRRHCERVFQVQGPADLRREWLDGARVAGLTAGTSTPDDVIDAVEARLREMAGG
                     GERSCWPGAGR"
     gene            complement(5068..6051)
                     /locus_tag="PKK95_09560"
     CDS             complement(5068..6051)
                     /locus_tag="PKK95_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18504.1"
                     /translation="MRRGLAALACFFVALGVLDRGLSWTMLALESRYYSGGNQFERQL
                     AGYLSGRNFDTLVLGTSRTSEGIIPLLLEAEGRPRISSVFKEAYQGKGPRYQLEFYRL
                     FRKYAGRPRLLVYGVDYFLYSIQSDRRWMSRFSSAESWKPPAPRRAPLLLLQRKHQHD
                     VFFNDLIDDINDRAGRKVAPATDTFEQSRRYLGVPADRSRLVIEKPLSYKRSRMPRPP
                     GPEGAAFERLLAETGADGVPVVLVSLPDYVGTYRTNVELATFHEHLRELSRRYPHVTV
                     LIYNDPRRFDLDDPSLFIDGGWGRTNSHLSTAGAAELSRMLARDLARLPAR"
     gene            complement(6053..7432)
                     /locus_tag="PKK95_09565"
     CDS             complement(6053..7432)
                     /locus_tag="PKK95_09565"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOC18505.1"
                     /translation="MRWAPLLAASLFFVGFARPVDLVPLALATAVTYGIALRLASLPA
                     WSTAARRRWLLLGTVALVGGLAAAKYLNFLEEMVSQAAALFGARLSLQPVHLLLPLGI
                     SFYTFRLLGYLLDVYHRRVEAERHAGYFGLFAAFFPQVSAGPIERARDFLPRLKSPER
                     FDIDRLASGATQVAWGLFKKAVIANRLAVFVDQVFAAPPGQGLNLVVAAWLYAVQIYC
                     DFSGYTDIAIGLSRLLGYQSAPNFDAPYASRSVAEFWTRWHMSLSFWFRDYLFLPLSY
                     AATRRERRDTFLGLRVETLAYVQATAVTMVLCGLWHGASWSFVTWGALHALYLIGGQL
                     SRRVRRRVARRTGLASRPSLHRALQVVVTFNLVSFAWIFFRAPTLAKALEYLHYMQIK
                     PSATGMGHMIVGVGLAAAFLLAEWICRRAAPFRPLPLPVRAGLLAATACAIVLFGVDA
                     GNEFIYVRF"
     gene            complement(7574..8044)
                     /locus_tag="PKK95_09570"
     CDS             complement(7574..8044)
                     /locus_tag="PKK95_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOC18506.1"
                     /translation="MPFPIGAHVRVTTIGKAGRVMSVSRGGRYRVLVGAVEVACRERD
                     LEAPSPAGGKRKPSAPAPPPAPRPAAAERPSDHERTRLGSIDLHGATVEEALRAVEAR
                     ADEALRAGLAHLDIVHGLGTGKVRHAVHNLLRAIGAVRYFEVTPGNPGVTRAYF"
BASE COUNT         1266 a         2819 c         2919 g         1205 t
ORIGIN      
        1 caggcctccc gcctgtgtgg ctgtggccag cagattctac tgcaccgaat cctgaccgcc
       61 gagtcgcgaa ctctgaaccc cgaatcccga ttcccgaatc ctgattcctg aatcctgatt
      121 cctgaactgg cgcgcctctt gcctgtgcct cgtgcatgct ggagaggatt cgtgtgtacg
      181 cgcgggcggt ggcgcgcttc ctcgaggcgc gatcgcctga agagcgccgg aaacgcgcca
      241 accacgcctt cgcgctgctg ctggtggccg gcgtggccgt catcaagcac gtcaccgggc
      301 tgacggacga gcgtgcacca ttcacgctct acgtgctggc cgtggcggcc gctgccgcgc
      361 gcggcggttt cgagtcgggc ctggtggcca cgctcgcctc ggttctcgcc gccgggttcg
      421 gcgcggctct cctcgggccc tcccgcctgc tgttcgcggc cgagggactg ctggccgccc
      481 tcgtcgtctc caccgtgcgt tcatcggtca ggatgaggga ggcgcagctg cggggggccg
      541 aggaaacgat cgccgacctg cgggcgcgcg accgccacgc gcagcggctc gacgccgcgc
      601 gccggcagga gtgggaggag taccgcgagg cggtggcacg ggcccaggcc gctctccagg
      661 aggcggccga cggcgcccgc aggcagcttg gcgccctgga aagcctcacc gatccgtcgc
      721 tgaacccgct cggcggcgac gacatggtgt cggagctgct cgcgcggctt cgcggggccg
      781 ttggtgcgga cgccgcggcg ctggtgcagc ctggcaccca ggggacggcc gtcgtgactg
      841 tcggtgcgct ggaaccggcc gccgggccga tcgacaccgc tgggcagcgg ctcgcccagg
      901 gccgggtggc ggtggtgcac aacgacccgg cgcgcgtcgc ccagctcagc gccctgcgct
      961 ggcccgactc ggtcacctcg ctgctggtgg tgccggtgct tcacgacggg caggtctggt
     1021 ccaccatcga ggtggtgagc gagcgatcgc gccaggtcag cgactgggac gtggcgctcg
     1081 cgcgtgtcgc ggccgaccgc ctggccgccg tcgtcgtgca ggggcgagcg ctcgcctcgc
     1141 gcgcgtcgtg acggctgggg cgagctggga agcttccctc aggaggccag tttcaggccg
     1201 agcactccgg ccacgatgag cgcgatgcag gagagccgcg ccgccgaggc aggttcctgg
     1261 aacagccaga tcccgagcag cgcggttccg gccgcgccga tgcccgtcca gactgcgtaa
     1321 gccgtgccga ttggcagcgt gcgcgccgcc gccgcgagca gcgccatgct cgcggccagc
     1381 gccgccagcg tccacgccgt cggccagagc cgcgtgaagc cgttcgtgta cttcagcccc
     1441 accgcccacc cgacctcgaa caacgccgcc acgagcagga tcagccacgc catcagaaga
     1501 gtctagggca cgatcacggc cggcgggagg ccggctcgat gttccggttg gtgcgaaaca
     1561 cgttctgcgg gtcccacgcg gccttgatct cggcgagccg gcccaggctc gcgccgagcg
     1621 ccgcctccac ccggtcgggt ccctcgtctg ctgtgaggaa gttgatgtag ttccctccgg
     1681 tggagaagcg cttcatgtcg ccccaggcgg ttcgcgccca cccggcgttg tcgccgtcgt
     1741 cggcgggctg ctcccacgag gccgtcacgt tcaagacgta gcgggcatcc cggttcgccg
     1801 ccggcgagtg atcctcggcg aggtcgttca gcgcgccgcc gatctggaac aggatcacgg
     1861 cggagtgcgg tgaccggacg cgcgcggcgt gctcggcgac cttgtcgcac aaggccggct
     1921 cgatgcgggg caggtactcg ctcttccagt aatacctccg ccccttgggc tgcgtggcgt
     1981 cgagcagcgc ctggatctgc acgtacgggc ggcgcacgag ggtgtcaccg acaggcctgc
     2041 cgaaggcctt gatgggggcg accgcccgct cgccctcctc cggattcccg ctgtggcagg
     2101 cgaggagggc gacgatcggt ttcccgtgca tctcgggcgg aagccatggc gcgggaggcg
     2161 ccgggcgcat cagtgcgacg agcgtcagct cgcgcggcgc ctgctcggcc agcgtgcggt
     2221 acagctccag cacgcgggac gcctcgctcg caggccacgc caccagcccg ccaacgacct
     2281 ctgggccgac agggtagagt gagtactcga tgccggtgac gacgccgaag ttgccaccac
     2341 cgccgcggag ggcccagaag agatccgggt tctcgcgagc gtccgcccgt acgatccggc
     2401 tctcggcggt gaccagctcg aagcccttga cgttgtcggc cgtccacccc cagcgccgtg
     2461 tcaggtagcc gaagccgccg ccgagggtga gccccgcgac cccggtgagc gagacgaagc
     2521 cgagcacggc ggccagcccg tgcagctgcg tctcgcgatc cacgtcgccg agcaggcagc
     2581 ccgcctgcgc ccgggcgact tgcccgcgcg ggtcggtcca gaccccgcgc atcagcgaca
     2641 tgtcgagcat gagcgcgcca tcggccgcgg ccagccctgc gatgttgtgc ccgcctccct
     2701 tgatgcacag caggatgtcg tgctcgcggg cgaaacgcac gcaggcgacg acgtcggagg
     2761 cgccgcggca gcacgcgatc gctgccggct tccgatcgat catggcgttc cagagtgtcc
     2821 gcgacgcctc gtatccgggg tcggcgggca ggagcaggag gcctcgcagg ttcgccttca
     2881 ggcggtcgaa cgcggccggc tccaggtcga tgtcgatgcc ctgcacagtc gttgctctca
     2941 tgacggggcg gcgggcagca agagtcgagc cagcatcccg atcacgttca ccgcagaggc
     3001 gcagaggtct tttggcttgc ggtgtcatag ctttgtgtgg caaagtaatc cgccatggag
     3061 cagcacgtcg cggccgaaca cctgcaggtc attcggacac tgatggagcg atccgcgctc
     3121 tatcgccgga ctctcgctcc gatcatgctg tatgcggggt cggtgggttg cgtggcctcg
     3181 gtcgggggaa tcctgctggg aatcgacgcc ctcccggcct tctcgatctg gtggctgatc
     3241 gcggccctcg tcgccattgg cggttcgttc gccatcgcca ggcgtcaggc cgtcgaggac
     3301 ggcgagccgt tctggtcgcc gccggcgctc cgcgtgacgc aggcgctcgc ccccccgctc
     3361 gcctccggcc tgctcctcag cctgggcttg atcgccttcg gcggcgacga cctccgctgg
     3421 gtgttcgtcc tggcgaacgc cttgttctac gggtgcgcgg ttcacgcggc cggcttcttc
     3481 atgccgcgcg ggatgaagct gttcggctgg ttgatcatcg cggccgcgtg cgcgggggtg
     3541 ctcgggcggc ccgccctcgg cggggtggcc acctcccgcc ccgaccacgc cctgatgggc
     3601 ggcctcttcg ggctcgcgca ccttggctac ggcgtctatc tctacgcgac ggagaaacga
     3661 aagacctcgt cgtgaacccc gaagccctcc tgcagctcga ccgcgtcatt cacgagaagg
     3721 ggcggctggc catcatgtcg atgctcgccg cgtcgcccga gttgtccttc accgagctgc
     3781 gtgagacgct ccagatgacc gacggcaacc tcacgaccca catccgcacg ctgcaggagg
     3841 cgggctacgt cgcggtggcc aaaagctatc gcaacaagcg ccgcctgacg accgtctcga
     3901 tgaccgaggc agggcgcgcg gccttcgccg cctacctcgc cctgctcgag cggatcgtgc
     3961 gggaggccaa accccgatag cacgttcgaa cgcaggagga aggcactgtg ctgccacatt
     4021 actttgtcac acaaagacga gaggagcatc cgatcccgct cgacgccgcc ccggcgcccg
     4081 actgtgtccc ggcgcccgac tgcgtcccgc cgtccgattc cgccccggcc gccgccgcag
     4141 cgcggcctga accggttcgg gtcgtgcggg cgagccacct gggcatgtgc tttggcgtcc
     4201 gcggtgccat cgccagggtc tttcgcatcg cgcatcgcga gccggtgacc gtgctcggcg
     4261 agctggtgca caacgccgcc gtcctcgatc gcctggcggc gagcggtgtc ctgttccagc
     4321 gcgaggcaga ggcggtcgag acgccggtcg tcgcgatcac cgcgcacggt gcctcgcgcc
     4381 gccgcctcgc ccacgtgcgc agcctgggct tgaaggtggt ggacgcaacg tgccccctgg
     4441 tgcgcaccgc gcaccaggcc gtgtcgcagt tcgtggagga ggggttccac ccggtcatcg
     4501 tcggcgtgcg cgaccacgtc gaggtgcgcg gcctgaccgg ggatctcgac gcctacgacg
     4561 tcgtggcgag cgaagcggac gtcgcccgcg tggccccacg gccgcgcttc ggcgtcgcgg
     4621 cgcagaccac gcaaccgatc gagcgggtgc ggcacctggc atccgtgctc caggctcgct
     4681 ttcccgggtc cgaggttcgc ctggcagaca ccgtgtgcct ccccacgaag ctgcgacagg
     4741 aggcggcagt agccctctcc accgagtgcg acgtggttat cgtggtcggc ggcgcgaaca
     4801 gcaacaacac gcgcgagcta tgcgagacct gccgccgcca ctgcgagcgc gttttccagg
     4861 tgcagggccc cgccgatctg aggcgggagt ggctcgatgg ggcgcgagtg gcgggcctca
     4921 ccgcgggcac ctccaccccg gacgacgtca tcgacgcggt cgaggccagg ttgagggaga
     4981 tggccggcgg aggggagcgc tcgtgctggc cgggagcggg ccggtagcag gttgccgaaa
     5041 caggaagcgt cagcggcctg caagccgcta cctggctggc aggcgcgcga gatcccgcgc
     5101 cagcatcctg ctcagctcag cagcgccagc cgtggacagg tgagagttgg tgcggcccca
     5161 gcctccatcg atgaacaggc tcgggtcgtc gaggtcgaat cgacgcgggt cgttgtagat
     5221 caacaccgtg acgtgtgggt accggcgcga caactccctc aggtgctcat ggaacgtcgc
     5281 aagctcgacg ttcgtccgat aggtcccgac gtaatcggga agggagacca gcacgacagg
     5341 tacgccgtcg gcgcccgttt cggccagcag tctctcgaaa gcggcgccct cgggcccggg
     5401 gggacgaggc atcctcgaac gcttgtagga cagtggcttc tcgatgacca gccggctgcg
     5461 gtccgccggc acccccaggt acctgcggct ctgttcgaag gtgtcggtcg cgggcgccac
     5521 tttccgtccg gcccggtcgt tgatgtcgtc gatcaggtcg ttgaagaaca cgtcgtgctg
     5581 gtgcttgcgc tgcaggagca gcagcggagc ccgccgcggc gcaggcggct tccaggattc
     5641 cgccgacgag aaccgcgaca tccagcgccg gtccgactgg atcgagtaga ggaagtagtc
     5701 cacgccgtag acgaggaggc gcggccgtcc ggcatacttt ctgaagaggc ggtagaactc
     5761 cagctggtag cgcggcccct tcccttggta cgcctccttg aagacgctgg agattcgggg
     5821 ccggccctcg gcctcgagca gcagcgggat gatgccttcg ctggtgcgcg acgtgccgag
     5881 gacgagcgtg tcgaagttcc ggccgctcag gtagccggca agctgccgct cgaactggtt
     5941 gccccccgag tagtagcgcg attcgagcgc cagcatcgtc cagctcaggc cccggtcgag
     6001 gaccccgagg gcgacgaaga agcaggcgag ggcggcgagg ccccggcgca cttcagaacc
     6061 ggacgtagat gaactcattg ccggcatcca cgccgaacag gacgatggca caggcggtgg
     6121 ccgcgagcag ccccgcgcgg acgggaaggg gcagcgggcg gaagggggct gcccttcggc
     6181 atatccactc ggcgagcagg aaggcggccg ccaggccgac gccgacgatc atgtggccca
     6241 ttccggtggc cgatggcttg atctgcatgt agtgcaggta ctcgagcgcc ttcgcaagcg
     6301 tcggcgcgcg gaagaagatc caggcgaacg agacgaggtt gaacgtcacg acgacctgga
     6361 gcgcccggtg aagcgacggg cgcgaggcca ggcccgttcg gcgggccacc cggcggcgaa
     6421 ctcgccggct cagctggccc ccgatcaagt agagggcgtg cagcgctccc caggtcacga
     6481 acgaccagct ggcgccgtgc cagagcccgc acagcaccat cgtcaccgcg gtcgcctgca
     6541 cgtaggccag cgtttcgacg cggaggccga ggaaggtgtc gcggcgctcg cggcgcgtgg
     6601 cggcgtagga cagcggcagg aacaggtagt cgcggaacca gaaggagagc gacatgtgcc
     6661 accgcgtcca gaactccgcc accgagcggc ttgcgtaggg ggcatcgaag ttcggcgccg
     6721 actggtatcc gaggagccgc gaaagcccga tggcgatgtc ggtgtagccc gagaagtcgc
     6781 agtagatctg gacggcgtac agccaggccg cgaccaccag gttgagtccc tgcccgggcg
     6841 gcgcggcgaa gacctggtcg acgaagacgg caagccggtt cgcgatcacc gccttcttga
     6901 acaagcccca cgccacctgg gtcgcccccg aagcgaggcg atcgatgtcg aagcgctcgg
     6961 gcgacttcag gcgcggcagg aaatcgcggg cccgctcgat ggggccggcg gatacctgtg
     7021 ggaagaaggc ggcgaagaga ccgaagtagc cggcatggcg ctcggcttcg accctgcggt
     7081 ggtagacgtc gaggagatac ccgagcaggc ggaacgtgta gaaggaaatg ccgagcggca
     7141 gcaacaggtg caccggctgc agggacagcc tggcgccgaa gagcgccgcg gcctgcgaaa
     7201 ccatctcctc caagaagttc aggtacttcg cggcggcgag cccgccgacg agcgcgacgg
     7261 tgccgagcag cagccagcgc ctgcgcgcag ccgtcgacca ggctggcagc gacgccagtc
     7321 tgagcgcgat cccgtaggtg acagccgtcg ccagcgccag cgggacgaga tcaacggggc
     7381 gcgcgaaccc aacgaagaac aggctcgctg ccagaagtgg agcccagcgc atccgcgcag
     7441 gcaaggacca ggcgaggcag aacgtcagga cgaagaaaag caggaaaccg gtcgattgga
     7501 ttgccacgag cagcaggcac tatacgaaac caggcgcctt ccgttcaatg gccaccccag
     7561 gcatttcgcc tgctcagaaa tacgcccgtg tgaccccggg gttgcctggc gtgacttcga
     7621 agtaacggac ggcgccgatg gcccggagga ggttgtggac cgcgtgccgc accttgccgg
     7681 tgccgaggcc gtggacgatg tcgaggtgag cgaggccggc acgcagcgcc tcgtctgctc
     7741 tggcctcgac cgcgcgcagg gcctcttcca cagtggcgcc gtggagatcg atcgacccga
     7801 ggcgggtgcg ctcgtggtct gacggtcgtt cggcggccgc gggccgtggc gccggcggcg
     7861 gcgcgggcgc gctgggtttc cgcttgccgc ctgccgggga tggcgcctcc aggtcgcgct
     7921 cccggcacgc gacctccacc gcgcccacga gtacgcggta gcgcccgccg cgggaaaccg
     7981 acatcacgcg ccccgccttg ccgattgtcg tcacgcgcac atgggcgccg atggggaagg
     8041 gcatggccag ttcgatctta cgccgcctct ggacgtgttc gaatgcgccg cgacgcatcg
     8101 gccgccattg ctgtccggat acctccgact ctgcggtgaa cgtgccggta caaacctcgc
     8161 atcgaagggc ccagcccgcg ggcacagatt ggctgcgccc ctacggggc
//
LOCUS       DAOPQX010000157         8155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_9832_length_8155_cov_4.027778, whole genome shotgun sequence.
ACCESSION   DAOPQX010000157 DAOPQX010000000
VERSION     DAOPQX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9832_length_8155_cov_4.027778"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKK95_09575"
     CDS             complement(<1..266)
                     /locus_tag="PKK95_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18507.1"
                     /translation="MLSFVQTRGRAAGLAVVAAVWLALGGLAMAAPQAPTGQADPQGY
                     AGEQTCLTCHEAQTKGYHDSAHGQAFIARAPINTLGCESCHGPG"
     gene            complement(642..2012)
                     /locus_tag="PKK95_09580"
     CDS             complement(642..2012)
                     /locus_tag="PKK95_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOC18508.1"
                     /translation="MSLRLRLRYLRHPVSLFGIWLTTVSAVMFLVVFAAELLGMHTNP
                     YIGIVFFLVLPGLFVLGLLLIPLGVWLMRRRERRGRPEGIEWPVVDMNVPHTRFLVMA
                     LLGATLVNIVVLSLAAYRGVEYMDSVSFCGQVCHTVMEPEYTAYQHGPHARVACVHCH
                     IGPGAPWFVKSKLSGTRQLFAVALHTYPTPVPSPVENLRPARETCEQCHWPEKFTGDR
                     IRQVFEYADDEPNTETVTTLRLHVGSGQQRLGATGIHWHMAPSTRVTYITTDPKRQVI
                     PYVRVEDARGRVVEYRAEGVTDEELAKGEERLMDCMDCHNRPSHRFDPTAATAVAEAM
                     SHGAIPKDLPFVHREAVEALQVKEPSPEAAAARIAKRLTTFYQANYPTVWNERRGEVE
                     AAVRAVQGIYARNVFPRMNVTWGTYPNNVGHVHAPGCFRCHDDTHRSADGQVIGQDCA
                     TCHTMD"
     gene            complement(2026..2838)
                     /locus_tag="PKK95_09585"
     CDS             complement(2026..2838)
                     /locus_tag="PKK95_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOC18509.1"
                     /translation="MTRSYLRFDRYHRILHGLLMISFIGLAFTGMPLLFSEASWAPYL
                     SAAFGGPWSAGIVHRVFAVVLIATFVLHVFRLLHRLFVKEDLEILWGPASMTPQPRDV
                     AEMIAHLRWFLRLGPRPEFDHFTYWEKFDYWAVFWGMGVIGTSGLMLWFPEFFSRFVP
                     GSWFNVALLVHGEEGLLAAGFIFTIHFFNSHLRPEKFPVDPVIFGGRVTEHELAEERP
                     AEYARLRAEGRLADHETTPALPWTFTLARVVAAVAVTLGLVMVVLILSSTWF"
     gene            complement(2835..4235)
                     /locus_tag="PKK95_09590"
     CDS             complement(2835..4235)
                     /locus_tag="PKK95_09590"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOC18510.1"
                     /translation="MRFRLALALAACCATAPPILAQQPAPPLTNDDCLACHGDPAAQG
                     SSGRSVAVDTSKFERSIHGQVGASCVDCHTDLAAMTELPHPEKLAPATCGACHEPAVQ
                     AYDLGIHAKARAGGKNPVAARCADCHGAHDILPSKDPDSRTYHLNLPGTCGRCHQNDE
                     IVQRGRIAGSDVSRFQDSIHGQALSRSGLLSAPNCADCHGYHDIRPPRDPASRVARVR
                     VPETCGSCHEGILNQYTGGVHGTALAGGNPLAPNCASCHTAHGVIRTESESWQLQVLA
                     ECGSCHAEAIRTYRDTFHGQVTRLGFVRVATCASCHGAHDILPAENPRSRVSPARLVE
                     TCGTCHPGATESFVQYDPHADRHNRERNPLLYYASRFMQTLLAGVFLFFGVHTLLWFS
                     RTAPLRRARKRAAARLRSDEEKRAAAAPAGGGERPGGAEEGGGGEGAAAGGETPGESG
                     EEGQGGAGTRGEGRDT"
     gene            complement(5024..6316)
                     /locus_tag="PKK95_09595"
     CDS             complement(5024..6316)
                     /locus_tag="PKK95_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18511.1"
                     /translation="MTMLNACELEIDLFCKGIRVPADVRLDGSRSISRTRAGLGSGLE
                     IVIPTGSRLKDRIWVNVPVEERFARQSPYRLEGSPEGGYAVRDERSQTSYTVEIPSEP
                     AWYTRETSRGIPMNRIGVLQGTYLGIYVNMVCTFWNYSPAVNCRFCTTGQNVGEHEVA
                     DKSVADVVEVARAAKAESGVTFVHLNAGFQGTRGVQFAEPYIRALKEEVGVMVGAQLA
                     PEKDFSRYDRLIALGVDHVSFCLEFVDPEWFARICPGKEKQLGQQLFFDSIQYCAARM
                     PRGAVSGEIIAGVEPIEKTLEGIDWIASQGAFPTVCIFRPTVGADMEDWPSPPYEDMR
                     RVMVHMYDACRRNWIPIGAAPNIEVSIVVNPDDTALLAPRTAGFYVFEGYRRAARLLA
                     APVFARRMRPRGRRESGATGENSPAGGAAPGGGGQVQA"
     gene            complement(6366..>8155)
                     /locus_tag="PKK95_09600"
     CDS             complement(6366..>8155)
                     /locus_tag="PKK95_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOC18512.1"
                     /translation="AVPEIEAVRKDGGRFPAEVSLRLSADGSVQAIVRDVTARYAAEA
                     ALRASEERFRRLYQYLPLAYQSLSPEGVILEVNEEWVRLMGLPREHAVGRRFDEMLPA
                     SGRARFGRLLTGLSGPAELRDIELEVVRATRPAAVVRLSGRLGHGAGGAHVLHCVLQD
                     VTAARAAAARVRESEARYRSLFDDSSASLWEEDFSGIKRHLDRLRALGVTDFEAHFDA
                     HPEELADCVGTVRVVDVNRATLAMYGAETRAQMLGGLDQIIGPDGYEVFRDGILALAR
                     GERSWQSEAVNYTLGGDQIVVALRWSLAPGAEESWSRVLVSAMDVTEQRRAESALRET
                     QALMEQAFRLAPGLMTLSTRFDGRYVDVNDAFLRELGYSRSEVVGRTASQLGLWVEPG
                     ARERLIAQFEEAGHLQNAEVRLRRRSGAVLDGLCSIAPLVVGGEPCLLIHIVDVTAWK
                     RAERAGRESERMLSTLMSNLPGMVYRCSDDPEWTMSFVSEGCRALTGYEPHELVGNRR
                     MSYGRVVLEEDRDAVINAVTEGVHAGRSFEIIYRIRRRDGAVRWVWEQGRAVGSGGGG
                     YALEGFIADISSFTEGAVPQPFVSGAVRR"
BASE COUNT         1277 a         2889 c         2789 g         1200 t
ORIGIN      
        1 ttgcccggcc cgtggcagct ctcacaccca agcgtgttga tcggggcccg cgcgatgaac
       61 gcctgcccgt gcgcgctgtc gtgatacccc ttggtctggg cctcgtgaca ggtgagacat
      121 gtctgctctc ccgcataacc ctgcgggtcc gcctggcccg tcggcgcttg cggggcggcc
      181 atcgccaggc ccccgagcgc cagccaaacg gccgcgacga cggcaagccc tgccgcacgc
      241 ccgcgggtct ggacgaatga aagcatggcc tcgctccttg tagtgaaaca caccaagcgg
      301 tccgtggacc tggaatgccg ggctgggctg tgcaacgaga gtgccgtgga gttccacatc
      361 acgaatgttg gctaattcac caaatgctcg cggaacgacc aactccaatt gttggatttc
      421 ggcacgttcc tgacgaaagc ccggcggttt ggctgcccga ccttccggct ttccggcaca
      481 tccgtatcca aaaccttgga gaagaggtct caaaacttcc agtttgtcga agaaaaccga
      541 ctcttcaggt cggcgcaagg caaggcggca gtgtcgagaa ttgagacagc tgtcgcatcg
      601 aacgatgctg gtcaacggcg acacgtgctc ctcgtgtcag ctcaatccat ggtgtggcag
      661 gtcgcgcagt cctgcccgat gacctggcca tcggcgctgc ggtgggtgtc gtcgtggcag
      721 cggaagcagc cgggcgcgtg cacgtgcccg acattgttcg gataggtccc ccaggtcacg
      781 ttcatgcgcg gaaacacgtt gcgcgcgtag atcccctgga cggcccggac ggcggcctcg
      841 acctcgccac ggcgctcgtt ccacaccgtg gggtagttgg cctggtagaa ggtcgtcagg
      901 cgtttcgcaa tccgggccgc cgccgcctcc ggcgagggct ccttcacctg gagcgcctcg
      961 actgcctccc ggtggacgaa cggcagatcc ttcggaatcg ccccgtgcga catcgcctcg
     1021 gcgaccgccg tcgcggcggt cgggtcgaac cggtggctcg ggcggttgtg gcagtccatg
     1081 cagtccatca gccgctcctc gcccttcgcc agctcctcgt cggtcactcc ctcggcccgg
     1141 tattcgacca cccgtccccg cgcgtcctcc acccggacgt aaggaatcac ctggcgcttc
     1201 gggtcggtcg tgatgtaggt cacccgggtc gagggcgcca tgtgccagtg gatgccggtc
     1261 gcgccgagcc gctgctgccc gcttcccacg tgcagccgca gcgtcgtcac cgtctccgtg
     1321 ttcggctcgt cgtcggcgta ctcgaagacc tggcggatgc ggtcgcccgt gaacttctcg
     1381 ggccagtggc actgctcgca ggtctcccgg gcggggcgca ggttttccac cggcgacggg
     1441 accggggtgg ggtaggtgtg cagcgcgacg gcgaagagct ggcgggtgcc cgacagcttc
     1501 gacttcacga accagggagc gccgggcccg atgtggcagt ggacgcacgc cacgcgggcg
     1561 tgcgggccgt gctggtacgc ggtgtactcg ggctccatca cggtgtggca cacctggccg
     1621 cagaaggaga ccgagtccat gtactcgacg ccccggtacg cggcgagcga gaggacgacg
     1681 atgttgacca gggtggcgcc gaggagggcc atgacgagaa accgcgtgtg ggggacgttc
     1741 atgtccacca cgggccactc gatcccctcg gggcggcctc gccgctcccg ccgccgcatc
     1801 agccacacgc cgaggggaat cagcagcagg ccgagcacga agaggccggg caggacgagg
     1861 aagaacacga tcccgatgta gggattcgtg tgcatcccca gcagctcggc ggcgaagacc
     1921 accaggaaca tcacggcgct gacggtggtc agccagatgc cgaacagcga gaccgggtgg
     1981 cggaggtaac gcaagcgcag gcgcagtgac atcgcggtct ccggctcaga accaggtcga
     2041 ggacaggatc agcaccacca tcaccaggcc gagcgtgacc gcgacggccg ccacgacgcg
     2101 cgcgagcgtg aacgtccagg ggagggccgg cgtggtctcg tggtcggcga gccgcccctc
     2161 ggcccggagc cgcgcgtatt cggccggccg ctcctcggcc agctcgtgct cggtgacgcg
     2221 gccgccgaag atcaccgggt ccacggggaa cttctccggc cgcaggtggc tgttgaagaa
     2281 gtggatcgtg aagatgaagc cggctgccag cagcccctcc tcgccgtgca cgagcagcgc
     2341 cacgttgaac cacgagccgg gcacgaaccg cgagaagaac tccgggaacc acagcatgag
     2401 cccggaggtc ccgatcactc ccatccccca gaacaccgcc cagtagtcga acttctccca
     2461 gtaggtgaag tggtcgaact cgggccgggg cccgaggcgg aggaaccacc gcaggtgggc
     2521 gatcatctcg gcgacgtcgc ggggctgcgg cgtcatcgag gccggtcccc acaggatctc
     2581 cagatcctct ttcacgaaga gccggtgcag gagccggaag acgtgcagca cgaaggtcgc
     2641 gatcaggacg acggcgaaca cgcggtggac gatgccggcc gaccagggcc ccccgaacgc
     2701 cgccgagagg tacggcgccc agctcgcttc gctgaagagc agcggcatcc cggtgaaggc
     2761 caggccgatg aagctgatca tcagcaggcc gtgcaggatc ctgtgatagc gatcgaaccg
     2821 caggtagctc ctcgtcatgt gtcccgcccc tcgccgcgcg tgccggcgcc gccctggcct
     2881 tcctcgccgc tctcgccggg ggtctcacct cctgcggccg ccccttctcc gccaccgcct
     2941 tcttcggccc cgccgggccg ttcgccgccc ccggccggcg cggccgccgc ccgcttctcc
     3001 tcgtcggacc gcaggcgtgc ggccgcacgc ttcctggccc ggcgcagcgg cgccgtgcgt
     3061 gagaaccaga gcagcgtgtg cacgccgaag aagaggaaga cgccggcgag cagcgtctgc
     3121 atgaaccggg aggcgtagta gagcagcggg ttccgctcgc ggttgtggcg atcggcgtgg
     3181 gggtcgtact ggacgaagct ctcggtggcg cccggatgac aggtgccgca cgtctcgacc
     3241 aggcgagccg gcgagacgcg cgaccgcggg ttctcggccg gcaggatgtc gtgcgcgcca
     3301 tggcagctcg cgcaggtcgc cacccgcacg aaccccagcc gcgtgacctg cccgtggaag
     3361 gtgtcgcggt aggtcctgat cgcttcggcg tggcacgagc cgcactcggc cagcacctgc
     3421 agctgccacg actcgctctc ggtccggatc acgccgtggg ccgtgtggca cgacgcgcag
     3481 ttcggggcga gcgggttccc gccagcgagc gcggtgccgt gcacgccgcc ggtgtactgg
     3541 ttcaggatcc cctcgtggca gctgccgcag gtctcgggca cccggacacg cgccacgcgg
     3601 ctggcggggt cccggggcgg ccggatgtca tggtagccgt ggcagtcggc acagttgggg
     3661 gccgacagca ggccgctgcg cgacagcgcc tggccgtgaa tgctgtcctg gaagcgcgac
     3721 acgtcgctgc cggcaatccg gccccgctgc acgatctcgt cgttctggtg gcatctcccg
     3781 caggtgccgg gcaagttcag atggtaggtc cgcgaatcgg gatccttcga gggcaggatg
     3841 tcgtgggcgc cgtggcaatc ggcgcatcgg gccgccactg ggttcttccc gcctgcccgc
     3901 gccttcgcgt ggatgccaag gtcgtaggcc tgcacggcgg gctcgtggca cgcgccgcag
     3961 gtggcgggcg ccagcttctc ggggtgcggc agctcggtca tggccgccag gtcggtgtgg
     4021 cagtccacgc acgacgcgcc gacctgaccg tggatcgacc gctcgaactt cgacgtgtcg
     4081 acggccaccg accggccgct gctcccctgg gcggcgggat cgccgtggca cgccaggcaa
     4141 tcgtcgttgg tcaggggagg cgccggctgc tgcgccagga tgggtggcgc cgtcgcgcag
     4201 caggcggcga gcgccagggc cagacggaat cgcatcgggt ctcgcgtcct cggccgggga
     4261 ggagcgcctc ccgccagcca tccgagcgcg ctgcggcgcg cgcgtgcgtt acgcggttgg
     4321 gatgagaacc accgcattgt aatcgtgacc gcagacgccg ccaagcccgt tggcggccgt
     4381 caaggtgcac tgcgggcgtc ggcggaaaca aaaagggcgg cctcggtggc cgccctcgaa
     4441 tttgcgtgcc cggtggggcc gccctgtgga actagggtct ggagggttta atgatagcgg
     4501 ttcgacacga ccggccgttt catgggctga ccggccgcgg cgggccaaaa gaggatacga
     4561 acgcgcgacc tcaccgggca ctgcattgtc atcgaagttg gttggagcgt tgttctgccc
     4621 tttccactct tttggacgca aacgaagtgc caccccccaa cccgcagaat ctcaaggaat
     4681 tcaaggctcc caagaacggg tgtaacggac cggcggcaat ttcaggtgac ggcctgacgg
     4741 gatgtcggca ggcctcacgg ctcagggctc aggggcctgc tcgccaacgc cgcagcctgc
     4801 gcccgccgaa gccgcgaggg gagcgagcgt ccggccgaag gcgggggcga agcgggcgcc
     4861 tggcgaaggc ggatggaagg cccggacaaa atagaatcgg cgccgtgaac cggacgcaca
     4921 catgcgggcc cccatccgca tgcgccgtgc ctgctcgcgg cgccgacacc cttctcggaa
     4981 cgcaaggcag gtgccactgc cctctcccga gaatccctcg aaattacgcc tggacctggc
     5041 ccccgccgcc cggcgcggca ccgccggctg gcgagttctc gccagtcgcg ccggattctc
     5101 gtcgcccccg cggcctcatc cggcgcgcga acaccggcgc cgccagcagg cgcgccgctc
     5161 tccggtatcc ctcgaacacg tagaagccgg ccgtccgcgg cgccagcagc gcggtgtcgt
     5221 ccgggttgac gacgatgctg acctcgatgt tcggggcggc gccgatcggg atccagttgc
     5281 ggcggcaggc gtcgtacatg tgtaccatca cgcgccgcat gtcctcgtac gggggcgacg
     5341 gccagtcctc catgtcggcg ccgacggtcg ggcggaagat gcagaccgtc gggaacgcgc
     5401 cctgcgacgc aatccaatcg atcccctcga gcgtcttctc gatcggctcc accccggcaa
     5461 tgatctcccc cgacacggcg ccgcgcggca tcctcgcggc acagtactgg atcgagtcga
     5521 agaacagctg ctggcccagc tgcttctcct tcccggggca gatgcgggcg aaccactcgg
     5581 gatccacgaa ctccaggcag aacgagacgt ggtccacgcc cagggcgatc agccggtcgt
     5641 agcgggagaa gtccttctcg ggcgcgagct gcgccccgac catcacaccg acctcttcct
     5701 tgagcgcccg gatgtacggc tcggcgaact gaacgccgcg ggttccttga aaccccgcgt
     5761 tgagatggac gaaggtcacg cccgactccg ctttcgcggc ccgcgcgacc tcgaccacgt
     5821 cggcaaccga cttgtcggcg acctcgtgct cccccacgtt ctggcccgtg gtgcagaacc
     5881 ggcagttcac ggccgggctg taattccaga acgtgcagac catgttgacg tagatgccga
     5941 ggtaggtccc ctgcaggacg ccgatgcggt tcatcgggat gccgcgcgac gtctcgcggg
     6001 tgtaccaggc gggctcggat gggatctcga cggtgtagga cgtctggctg cgctcgtcgc
     6061 gcaccgcgta gccgccctcg ggcgagcctt cgagccggta gggagactgg cgggcgaagc
     6121 gctcctcgac cggcacgttc acccagatcc ggtccttcag ccgggagccg gtggggatca
     6181 ctatctcgag gcccgacccc aggcccgcgc gggtgcgcga gatgctgcgc gagccatcga
     6241 gccggacgtc ggccggcacc cggatgccct tgcagaacag gtcgatctcc agctcgcacg
     6301 cgttgagcat cgtcacccct cttcccgccc ggccgtggaa catgcggggc cggccgcccc
     6361 ccggctcacc gccgcaccgc cccggagacg aagggttgcg gcaccgcgcc ctcggtgaag
     6421 ctgctgatgt cggcgatgaa cccctcgagc gcgtaccccc caccgcccga cccaacggcg
     6481 cgcccctgct cccagaccca gcgcaccgcg ccgtcgcgtc gccggatccg gtagatgatc
     6541 tcgaaagaac gccccgcgtg caccccctcg gtgaccgcgt tgatgacggc gtcgcggtct
     6601 tcctcgagca ccacccgccc gtaggacatc cggcggttgc ccaccagctc gtgcggttcg
     6661 tagccggtca gcgcgcggca cccttcgctg acgaatgaca tcgtccactc cgggtcgtcc
     6721 gagcagcggt acaccatgcc ggggaggttg ctcatcagcg tggagagcat gcgctcgctc
     6781 tcgcggccgg cccgctcggc ccgcttccag gcggtcacgt cgacgatgtg aatgagcagg
     6841 cagggctcgc cgccgaccac cagcggcgcg atcgagcaga ggccgtcgag gacggcgccc
     6901 gagcggcgcc gcagccgaac ctcggcgttc tgcagatgcc ccgcctcctc gaactgggcg
     6961 atcaggcgct ctcgtgcgcc gggctcgacc cacagcccca gctggctggc ggtgcggccc
     7021 acgacctcgc tgcgcgagta cccgagttcg cgcaggaagg cgtcgttgac gtcgacgtac
     7081 cggccgtcga aacgggtgct gagcgtcatc aagcccggcg ccagacggaa cgcctgctcc
     7141 atcagcgcct gggtttcacg cagcgccgac tcggcgcgcc gctgctcggt gacgtccatg
     7201 gccgagacca gcacgcgcga ccacgactct tccgcgcccg gggccagcga ccagcggagg
     7261 gccaccacga tctggtcccc gccgagggtg tagttcaccg cctcgctctg ccaggaccgc
     7321 tcgccccggg cgagggccag gatcccgtcc ctgaagacct cgtagccgtc cgggccgatg
     7381 atctgatcga ggcccccgag catctgcgcc cgcgtttcgg cgccgtacat ggccagcgtc
     7441 gcacgattca cgtccacgac gcggaccgtc ccgacacagt cggccagttc ctcggggtgc
     7501 gcgtcgaagt gggcctcgaa gtcggtcacc ccgagcgccc gcaggcgatc gagatgccgt
     7561 ttgatcccgg agaagtcctc ctcccagagc gatgccgagc tgtcgtcgaa cagcgagcgg
     7621 tatcgtgcct cgctctcgcg gacccgcgcc gccgccgcgc gcgccgcagt gacgtcctgc
     7681 agcacgcagt gcagcacgtg cgcgccgccg gctccgtgcc ccaggcggcc gctcaggcgc
     7741 acgacggcgg ccgggcgcgt cgcgcgcacc acctcgagct cgatgtcgcg cagctcggcc
     7801 ggcccgctca ggccagtcag cagacgcccg aaccgggcgc gccccgaggc aggcagcatc
     7861 tcgtcgaagc ggcggcccac ggcgtgctcg cgcggcagcc ccatcagccg cacccattcc
     7921 tcgttgacct cgaggatcac cccctcgggc gagagcgact ggtaggcgag cggaaggtat
     7981 tggtagaggc ggcgaaaacg ctcctcgctc gctcgaagcg ccgcctcggc cgcgtagcgg
     8041 gcggtcacgt cgcggacgat ggcttgcacc gatccatcgg ccgacaaccg caggctgacc
     8101 tcggccggga agcggcctcc gtccttccgc accgcctcga tctcgggaac cgcgc
//
LOCUS       DAOPQX010000158         8046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10015_length_8046_cov_4.683519, whole genome shotgun sequence.
ACCESSION   DAOPQX010000158 DAOPQX010000000
VERSION     DAOPQX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10015_length_8046_cov_4.683519"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="trmFO"
                     /locus_tag="PKK95_09605"
     CDS             complement(<1..1048)
                     /gene="trmFO"
                     /locus_tag="PKK95_09605"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOC18513.1"
                     /translation="MSEGRLTVVGGGLAGCEAAWQAASRGVRVTIHEMRPARPTEVHR
                     TADLAELVCSNSLRSDKLDNAVGLLKEEMRRLDSLVMRVADEVRVPAGAALAVDRERF
                     AARITEEVTRHPLIDVVRGEVTELPDGASPTTPTIVATGPLTSETLAARIVPLVGEAS
                     LYFYDAVSPIVLADTIDLDRVFRASRWGRGRRQPAASNQPAASPAGALREVAGDEVAE
                     SDGDYLNCAFDEAEYLRFYEALMAAERAPLHDFEEGRFFEGCLPIEVMASRGPDTLRF
                     GPMKPAGLADPRTGRRPYAVVQLRQDNLAADHYSLVGFQTQLKWGEQARVFRLIPGLE
                     NAEFVRFGLVHRNTY"
     gene            complement(1045..3744)
                     /gene="topA"
                     /locus_tag="PKK95_09610"
     CDS             complement(1045..3744)
                     /gene="topA"
                     /locus_tag="PKK95_09610"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOC18514.1"
                     /translation="MIVESPAKVKALSRFLGPDYRVEASFGHVRDLPESAEEVPAEAK
                     KLLGPDSNLGVDVKNDFRPFYVIPSAKKRHVATLRSALKEASQVLLATDPDREGESIS
                     WHLKEVLKPRVPARRITFHEITREAVRRAVQEAHDIDENLVRAQESRRILDRLFGYTL
                     SPVLWRKVRTGLSAGRVQSVAVRLLVEREEARRAFRVSTYWDIEAKLAAEGREFTAVL
                     VRVGDERVATGRDFDPATGALASRGVRHLDEASAARISEALRANLPWQVTNVEVRPGT
                     ERPAPPFTTSTLTQEASRKLGFSTSRTMRIAQRLKDGVELPDGTIEGIITYHRTDSTT
                     LSEKALQESGRAIREMFGGEYYKGPRRYQTQVRNAQEAHEAIRPTDFTLTPQQLANVL
                     EPDELRLYDLIWKRTMASQMPDARVLKTTVEFTGRGPGGEPCALTVSGKAIEFAGFRR
                     AYVEGSDDPDSELEAQETILPAFKEGDLVAAPGWTGGQAVANVRDIDPKRHETTPPPR
                     YTEASLIKKLEEEGIGRPSTYEPTIETILKRGYVFRQGRALVPSFTAFAVTRLLRDHF
                     GDFVDVGFTAEMEKDLDEISNGERAWVDFVREFYRGDGKKRKGLEGLVREGETQIDYP
                     VIDVGDDPDTGQPIKVRIGRFGPFLQVGEGGPGNTASLPDDLPPADLTVDKARELVRV
                     KAAGPRELGTDPATGQKVYLASGRFGPYVQLGETPEKPAKGAKAEKPRRASLPRGESE
                     PDVTLEKALRWLSLPRELGRHPETGAQVQASIGRFGPYVKHGDEFRSLAPTDDVYTIT
                     LERAVALLAEPKQPRRRQAAAKTVLRELGARDDGRAVKLFDGRYGPYVSDGTTNASVP
                     KGTDPMALTLSEATALLDARAGAPRRRPAARRTTGRKRAAGAA"
     gene            complement(3797..>4872)
                     /gene="dprA"
                     /locus_tag="PKK95_09615"
     CDS             complement(3797..>4872)
                     /gene="dprA"
                     /locus_tag="PKK95_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOC18515.1"
                     /translation="DTVALSLWSLAARRRQGPPFHPLLATSAPRAGDDPPDSLPAMLE
                     CAGACASESLARDLREQAQAALERARAAGIDPVPWGDARYPPLLAAIPDPPVVLWTRG
                     DAGCLARPAVAVVGSRAASEYGLEAASRLAGELAGAGMVIVSGLARGVDSAAHRGALG
                     ADGLTVAVLGSGVDVIYPAEHASLATAIALHGAVVSEYPPGTPPLPQYFPLRNRIISG
                     LCLGVVVIEAAERSGSLITANCASDQGREVMAVPGNILSGRFRGSHALLRDGAAVVDE
                     ASDVLAELGRSSLQALSGKGAGGEPPPDPVLAAMAAGEAYDLDALALGTGLAPPALLP
                     RLLDLELRGWVRRVGGGRFMRAR"
     assembly_gap    4873..4972
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5031..5384
                     /locus_tag="PKK95_09620"
     CDS             5031..5384
                     /locus_tag="PKK95_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC18516.1"
                     /translation="MRTALATLFIVGLALTPALRADSRSEARAQVEFGIAVAQKGLWK
                     EALFRWEKAVQLDPTYAAAYNNLAIAYEHEGQFEKARQAYEKAAELDPKSTFIRQNYE
                     FFKEINDRTGARAAR"
     gene            5353..6123
                     /locus_tag="PKK95_09625"
     CDS             5353..6123
                     /locus_tag="PKK95_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18517.1"
                     /translation="MTARALALLASLAVSGAGCTSYYEIAIETPIQPKLDVTPFQRVL
                     VAGFIAGGTDEVDANTETVRLLRSQLRTKSSLRVIDAEVVPLTEVASEQPSADEGTPA
                     NGVEPAKAPKSIDDEKDLEAYDHVFANAAFWKKLGEEYQNPLIVTGTILFTPHQRAGF
                     VQREREVVDAYGRRSVSPTRTYMERKGFILRPKFIFIDGRTGTPLYSETFREEVLYNP
                     QQDTPPLSSYFELMDRLIPGFLGTLSSQKIKGSRVLLK"
     gene            6423..6734
                     /gene="rplU"
                     /locus_tag="PKK95_09630"
     CDS             6423..6734
                     /gene="rplU"
                     /locus_tag="PKK95_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOC18518.1"
                     /translation="MYAIVQCGGHQVKMVPGESVVVDRLDGEAGSEVSFDQVLVVERD
                     GGELVAGAPFVAGARVVAVVDGEARGPKIRVFKKKRRKGMRRTKGHRSTYTRVTVKEI
                     V"
     gene            6734..6991
                     /gene="rpmA"
                     /locus_tag="PKK95_09635"
     CDS             6734..6991
                     /gene="rpmA"
                     /locus_tag="PKK95_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOC18519.1"
                     /translation="MAHKKGQGSSRNGRDSNAQRLGVKRFDGNVVTGGSILVRQRGRR
                     FQPGLNVGLGKDDTLFAKITGRVQFSDHGAHGRKISVLPIE"
     gene            7076..>8046
                     /gene="obgE"
                     /locus_tag="PKK95_09640"
     CDS             7076..>8046
                     /gene="obgE"
                     /locus_tag="PKK95_09640"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOC18520.1"
                     /translation="MFVDEVDIRVSAGAGGRGAVSFRREKFVPRGGPNGGDGGNGGSV
                     YLAASPHLNTLVSFRFHPEFHGRRGAHGEGSNRTGRDGGDLVIEVPIGTVVFRSPIEG
                     GEPEMVADLDHEGARVLVARGGRGGRGNARFATSTNRAPRRAEPGEPGEAWQLRLQLK
                     LLADVGLVGFPNAGKSTLISRISAARPKIADYPFTTLTPNLGVVRLSDDRSFVVADVP
                     GLIEGAHRGAGLGYQFLRHVERTKVLVHVVDVSSASGREPARDLAVIRRELDLFHAPL
                     LDRPQVVAATKVDVLDEPERLESLRAAAASLDLPVFPVSGVTGQGVG"
BASE COUNT         1142 a         2858 c         2765 g         1181 t
ORIGIN      
        1 tgtaggtgtt gcggtgcacc aggccgaacc ggacgaactc ggcgttctcg agcccgggta
       61 tgagccggaa cacgcgcgcc tgctcgcccc acttcagctg cgtctggaag ccgacgaggc
      121 tgtagtgatc ggccgcgagg ttgtcctgcc gcagctgcac cacggcgtac gggcggcggc
      181 cggtgcgtgg atccgcgagg ccggccggct tcatggggcc gaaccgcagc gtgtcggggc
      241 cgcgcgacgc catcacctcg atcggcaaac acccctcgaa gaagcggccc tcctcgaagt
      301 cgtgcagcgg cgcacgctcg gccgccatca aggcctcgta gaagcgcagg tactcggcct
      361 cgtcgaaggc gcaattcagg tagtcgccgt cactctccgc cacctcgtcg cccgccacct
      421 cacgaagcgc gcctgcaggg gatgcggccg gctggttcga ggctgccggc tgccggcgcc
      481 cccgtcccca ccgcgacgcc cggaacacgc gatcgaggtc gatcgtgtcg gccaggacga
      541 tcgggctcac cgcgtcgtag aagtacagcg acgcctcgcc gacgagcggg acgattcgtg
      601 ccgcgagcgt ctcggacgtc agcgggccgg tggcgacaat cgtgggagtg gtgggtgaag
      661 cgccgtcggg cagctcggtc acctcgccgc ggaccacgtc aatcagggga tgccgcgtca
      721 cttcctcggt gatccgcgcg gcgaaccgct cgcggtccac cgcgagcgcg gccccggcgg
      781 gcacgcgcac ctcgtctgcc actcgcatga cgagcgagtc gaggcggcgc atctcctcct
      841 tgagaaggcc cacggcgttg tcgagcttgt cgcttcgaag ggagttgctg cacaccagct
      901 cggcgagatc cgccgtacgg tgcacctcgg tcggccgcgc agggcgcatc tcgtggatcg
      961 tcaccctcac gccgcgcgat gcggcctgcc acgcggcctc gcagccggca aggccgccgc
     1021 cgaccacggt cagccgcccc tcgctcacgc cgccccggcg gcgcgcttgc gtcccgtggt
     1081 cctgcgcgcg gccggcctcc gccgtggcgc ccccgcccgc gcgtcgagca gggcggtcgc
     1141 ctcgctcagt gtcagtgcca tcgggtcggt acctttgggc accgacgcgt tggtcgtgcc
     1201 gtccgaaacg tacgggccgt accgaccgtc gaacagcttc accgcacggc cgtcgtcccg
     1261 cgcccccagc tcgcgcagca ccgtcttggc ggccgcctgc cgccgccggg gctgcttagg
     1321 ttcggcgagc aacgccaccg cccgttcgag cgtgatggta tagacgtcgt cggtgggggc
     1381 cagcgagcgg aactcgtcgc cgtgcttcac gtacggcccg aagcggccga tgctggcctg
     1441 gacctgcgcg ccggtttcgg gatgccgccc cagttcgcgc ggcaggctga gccagcgcag
     1501 cgccttctcc aacgtgacgt ccggctcgct ctcgccgcgc ggcagcgagg cccgccgcgg
     1561 cttctcggcc ttcgcaccct tggccggctt ctccggcgtc tcgccaagct gcacgtaggg
     1621 gccgaagcgg ccgctggcca ggtagacctt ctgcccggtg gccgggtcgg tgcccagctc
     1681 gcgcgggccg gcggccttga cgcgcaccag ttcgcgcgcc ttgtccacgg tgagatcggc
     1741 gggcggcagg tcgtcgggca gcgaggcggt gttgcccggc cctccctcgc ccacttgcag
     1801 gaacggtccg aaccggccga tccgcacctt gatcggctgc cccgtgtcgg ggtcgtcgcc
     1861 gacgtcgatc accgggtagt cgatctgcgt ttcgccctcg cgcacgagcc cctcgagccc
     1921 cttgcgcttc ttcccgtcgc cgcggtagaa ctcccggacg aaatcgaccc acgcccgctc
     1981 gccgttcgag atctcgtcga ggtccttctc catctcggcg gtgaagccga cgtcgacgaa
     2041 gtcgccgaag tggtcgcgga gcagcctggt caccgcgaag gcggtgaagc tgggcacgag
     2101 cgcgcgcccc tgcctgaaga cgtagccgcg cttgaggatc gtctcgatgg tcggctcgta
     2161 ggtggacggc cggccgatcc cctcctcctc gagcttcttg atcagcgagg cttccgtgta
     2221 gcgcggcggc ggtgtggttt cgtggcgctt cgggtcgatg tcgcggacgt tcgccaccgc
     2281 ttgcccgccc gtccatccgg gcgccgccac caggtcgcct tccttgaagg cgggcaggat
     2341 ggtctcctgc gcctcgagct cgctgtccgg gtcgtcgctt ccctccacgt acgcgcggcg
     2401 gaacccggca aactcgatcg ccttgccgct gaccgtgagc gcgcacggct cgccgccggg
     2461 cccgcgcccg gtgaactcca cggtcgtctt cagcacccgg gcgtcgggca tctgcgacgc
     2521 catcgtccgc ttccagatca ggtcgtagag ccgcagttcg tcgggctcga ggacgttcgc
     2581 aagctgctgc ggcgtgaggg tgaagtcggt gggcctgatc gcctcgtgcg cctcctgcgc
     2641 gttgcggacc tgcgtctggt accgccgcgg acccttgtag tactcgccgc cgaacatctc
     2701 ccggatcgcg cgccccgact cctgcagcgc cttctcgctg agggtggtcg agtcggtgcg
     2761 gtggtaggtg atgatcccct cgatcgtgcc gtcgggcagc tcgacgccgt ccttcagcct
     2821 ctgcgcgatc cgcatggtgc ggctggtcga gaacccgagc ttgcggctgg cctcctgggt
     2881 cagcgtcgag gtggtgaacg ggggggccgg ccgctcggtg ccgggccgga cctccacgtt
     2941 ggtcacctgc cagggcaggt tcgcccgcag cgcttccgaa atccgcgcgg cggacgcctc
     3001 gtcgaggtga cgcacgccgc gcgaggcgag ggcgccggtg gccggatcga agtcgcgccc
     3061 ggtggccacc cgctcgtccc cgacgcgcac gagcacggcg gtgaactcgc gcccctcggc
     3121 ggcgagcttt gcctcgatgt cccagtaggt gctgacgcgg aaggcacgcc gcgcctcctc
     3181 gcgctcgacc agcaaccgca ccgccacact ctgcacgcgc cccgcgctca gcccggtgcg
     3241 caccttcctc cagaggaccg gcgacagcgt gtagccgaag aggcggtcga ggatgcgccg
     3301 gctctcctgc gcacggacga ggttctcgtc gatgtcgtgc gcctcctgga ccgcgcgtcg
     3361 gaccgcctcg cgcgtgatct cgtgaaacgt gatccgccgc gccgggacgc gcggcttcaa
     3421 gacctccttg aggtgccagc tgatcgactc gccctcgcgg tcggggtcgg tcgcaagcag
     3481 tacctgcgac gcctccttga gggcagacct gagcgtcgcc acgtgccgct tcttcgccga
     3541 gggaatcacg tagaacggcc tgaagtcgtt cttcacgtcc acgccgaggt tgctgtcggg
     3601 cccgagcagc ttcttcgcct ccgcgggcac ttcctcggcg ctctcgggca ggtcgcggac
     3661 gtggccgaag ctcgcctcga cccggtagtc ggggccgagg aaccgcgaca acgccttcac
     3721 cttcgccgga gactcgacta tcaccaaaga cttcgccatg ccacctcttg cgacgttggc
     3781 aactacgtta gcacgttcac cgagcacgca tgaaccggcc gccgccgacg cgccggaccc
     3841 acccccgcag ctcgagatcg agcaagcgcg gcaggagcgc cggcggtgcg aggccggttc
     3901 cgagcgcgag ggcgtcgaga tcgtacgcct cgccagccgc catcgcggcc agcaccgggt
     3961 cgggcggcgg ctccccgccc gcacccttgc ccgacagcgc ctgcaggctg ctccggccca
     4021 gttcggccag cacgtccgat gcctcgtcga cgacggccgc accgtcgcgc agcagcgcgt
     4081 gcgagcccct gaagcgcccg gagaggatgt tccccgggac cgccatcacc tcgcgcccct
     4141 ggtcggacgc gcagttcgcc gtgatcagcg atccgctgcg ctcggccgcc tcgatcacca
     4201 cgacgccgag acagaggccg ctgatgatgc ggttgcgcag ggggaagtac tgcggcaacg
     4261 gcggcgtgcc cggcgggtac tcggacacga cggccccgtg cagcgcgatg gccgtcgcca
     4321 ggctcgcgtg ctcggccggg tagatgacgt cgacccccga ccccagcacg gcgacggtca
     4381 ggccgtcggc gccgagggca ccccggtgag ctgcggaatc gacgccccgc gcaaggccgc
     4441 tgacgatgac catcccggcg ccggcaagct caccggccag ccgcgacgcg gcctcgagcc
     4501 cgtactcgga cgcggcgcgc gagccaacga ccgccaccgc cggccgggcg aggcagccgg
     4561 cgtcgcccct ggtccacagc accacgggcg ggtcgggaat ggcggcaagc agcggcgggt
     4621 agcgggcgtc gccccacggc accgggtcga tgccggccgc cctggcgcgc tcgagcgccg
     4681 cctgcgcctg ttcgcgcagg tcacgggcca gcgactcgga tgcgcacgcc cccgcgcact
     4741 cgagcatggc cggaagcgag tcgggaggat cgtccccggc tcggggcgcg ctggtggcga
     4801 gcagcggatg gaagggtgga ccctggcggc gccgtgcagc cagcgaccag agggaaagcg
     4861 cgacggtatc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacgcgtgg
     4981 tttgcgcggc cccgggccga cgggctatag ttgaatcggt cccgactctc atgcgaacag
     5041 ccctggcgac cctgttcatc gtcggcctcg cgctgacgcc ggctcttcga gccgactcga
     5101 ggagcgaggc gcgcgcgcag gtcgagttcg gcattgccgt ggcgcagaag ggcctctgga
     5161 aggaagcgct cttccgctgg gagaaggcgg tccagctcga tcccacctac gcggcggcgt
     5221 acaacaacct ggccatcgcc tacgagcacg aggggcagtt cgagaaggcg cgccaggcct
     5281 acgaaaaggc ggcggagctc gaccccaaga gcacgttcat ccgtcagaat tacgagttct
     5341 tcaaggaaat caatgaccgc acgggcgctc gcgctgctcg ctagcctcgc cgtctcgggt
     5401 gccggctgca ccagctacta cgagatcgcg atcgagacgc cgatccagcc gaagctcgac
     5461 gtgacgccct tccagcgtgt cctggtggcc ggcttcatcg ccggtggcac cgacgaggtg
     5521 gacgcgaaca ccgagaccgt gcgcctgctt cggagccagc tgcggaccaa gtcgagcctg
     5581 cgcgtgatcg acgccgaggt cgtgccgctg accgaggtgg cctccgaaca gcccagcgcc
     5641 gacgagggga cgccggccaa cggcgtggag ccggcgaagg cccccaagag catcgacgac
     5701 gagaaggacc tcgaggccta cgaccacgtg ttcgcgaacg cggccttctg gaagaagctt
     5761 ggcgaggagt accagaaccc gctgatcgtc accgggacga tcctcttcac gccgcaccag
     5821 cgcgccgggt tcgtgcagcg cgagcgcgag gtggtggacg cctacggacg gcgctcggtg
     5881 tcgccgacgc gcacctacat ggagcggaag gggttcatcc tgcggccgaa gttcatcttc
     5941 atcgacggcc gcaccggcac gcccctctac tcggagacct tccgcgagga ggttctctac
     6001 aacccgcagc aggacacccc tccactgtcg tcgtacttcg agctgatgga ccgcctcatc
     6061 cccggcttcc tgggcacgct cagcagccag aagatcaagg ggagtcgagt tctcctgaag
     6121 tagatcgtgc agtgtgctat aggtgctgcc agcacccttc ggcacccgca ccttcggcac
     6181 ccttggcacc ttcagcacca gcaccttcag cacccctgca ccctttgcac ccccagcacg
     6241 accaggtgct ggattgtcgt gcggatcgtc tcccgcggtt ccaatcctct ggatcgtccc
     6301 ctccctccca tgctaattta gatgtttccc ggtgccccgg gtgccgcggc aggggtttgc
     6361 ccggccgggc ggggcgccgc gagggcggcg acccggccgc gaaaccgaca ggagatacag
     6421 gcgtgtacgc gattgttcaa tgcggcgggc accaggtgaa gatggtgccg ggcgagtcgg
     6481 tggtggtcga tcgcctcgac ggtgaggccg gttccgaggt cagcttcgac caggtgctgg
     6541 tggtcgagcg cgacggcggc gagctggtgg ccggggcgcc gttcgtggcc ggcgcccgcg
     6601 tcgtggcggt ggtggacggg gaggcgcggg gcccgaagat ccgggtcttc aagaagaagc
     6661 gcaggaaggg catgcgccgg accaagggac accgcagcac ctacacccgc gtcaccgtga
     6721 aggaaatagt gtaatggcac acaagaaggg acagggcagc tcgcgcaacg ggcgcgacag
     6781 caacgcgcag cgcctgggcg tcaagcggtt cgacggcaac gtcgtgaccg gcggatcgat
     6841 cctcgtccgg cagcgcggcc gccgcttcca gccgggtctg aacgtgggcc tcgggaagga
     6901 cgacacgctg tttgcgaaga tcaccggccg cgtgcagttc tcggatcacg gcgcgcacgg
     6961 ccgcaagatc agcgtcctgc cgatcgagta gacggccgga aggtgacacg atccgtgtcc
     7021 gcgaagctcg cgcggacgcg gagggtgtca ccttgttccc tcccgcccct gcaccatgtt
     7081 cgtcgacgag gttgacatcc gggtttccgc cggcgccggg gggcgcggcg cggtcagctt
     7141 ccgccgcgag aagttcgtcc cacgaggcgg cccgaacggc ggagacggcg gaaacggcgg
     7201 ctcggtgtac ctcgcggcca gccctcacct caacacgctc gtcagcttcc gcttccaccc
     7261 cgagttccat gggcgccgcg gcgcgcacgg cgagggctcg aacaggaccg gtcgtgacgg
     7321 cggcgacctg gtgatcgagg tcccgatcgg aacggtcgtg ttccgatcgc cgatcgaggg
     7381 cggcgagccc gagatggtgg ccgatctcga ccacgagggg gcgcgggtgc tcgtggcgcg
     7441 gggcggccgc ggcggacgcg gcaacgcccg gttcgccacc tcgaccaacc gggcgcccag
     7501 gcgcgccgag ccgggcgagc ccggcgaagc ctggcagctg cgcctgcagc tgaagctgct
     7561 tgccgatgtc gggctggtcg ggttccccaa cgcgggcaag tccacgctca tctcgcgcat
     7621 ctcggccgcg cgcccgaaga tcgccgacta ccccttcacg accctgacgc cgaacctcgg
     7681 cgtcgtacgg ctgtcggacg accgcagctt cgtggtggcc gacgtcccgg ggctgatcga
     7741 aggggcccac cgcggcgccg gcctcggcta tcagttcctc cgtcacgtcg agcggacgaa
     7801 ggtgctcgtg cacgtggtgg acgtctcgtc cgcgtccggc cgcgagccgg cgcgcgacct
     7861 ggccgtcatc cggcgcgagc tcgacctgtt ccacgcgccg ctcctcgatc ggccccaggt
     7921 cgtcgcggcg accaaggtcg acgtgctcga cgagcccgag cggctcgagt cgctccgggc
     7981 ggcggccgcg tcgctcgacc tgccggtgtt tcccgtgtcg ggcgtgacgg gccagggcgt
     8041 cggtga
//
LOCUS       DAOPQX010000159         8041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10022_length_8041_cov_4.365765, whole genome shotgun sequence.
ACCESSION   DAOPQX010000159 DAOPQX010000000
VERSION     DAOPQX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10022_length_8041_cov_4.365765"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKK95_09645"
     CDS             complement(<1..371)
                     /locus_tag="PKK95_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18521.1"
                     /translation="MGFFDKVTKAVGDAVDKTKREADQFMRIQKIKGEIGEVERKIAG
                     FTAEIAQVKADAGAKAIAMLRSGGLNAADLQPLADRVATLEQQIEAEQAVIAGKRADI
                     EKIKAEDEQPAADAAPPAPPAV"
     gene            complement(510..3128)
                     /locus_tag="PKK95_09650"
     CDS             complement(510..3128)
                     /locus_tag="PKK95_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18522.1"
                     /translation="MRRVLVVSLVLLGLAGSVSVAQQAAPKPGAPPPRGAAAAQQSAV
                     PAFDGDVAALAWGGRLESLSGAKDLNHAQGILAGDWSHAVEGKGPVEALVSFFERETV
                     LVRAVRLESLPHDLGMRDVEIWTTNAAAPEGFTKVSAATVAREPNAFKHAEVTLTFEP
                     VEARFVQVRFLNNHRDPKVAGNPYAFRIARIQVFEGQAPGYVPLLTRHPEIAEPVFMA
                     EGAKAAAAQRAPVAGCSPDADPDIDPGDGESRNVLLLMTNYLGGPSSFVPLGIESGRF
                     PKTYPSKWKELDIFGRIKTRLVALNHVQPWMLAGVDTVVMQQACNLKPLSPRFRKALT
                     AWVAAGHKLIIHDSDKCGSTPDYSWLPYRFKSDNPGAFGKPGSVLRIVENNWMAHTYR
                     TRPGFVDTAEWVSWPPPGNELGDSNAIMEWDPGWCGHMVVKNADGIFGVVQAYAHHGR
                     GLIIWDGLDVDMVGTRWMDVIHARQLSQGFNTDNLPCSIRVGNFIVTTEPRLGWRGVQ
                     PGKTYEYPLSLVSNLGYKGVVSLTASATPAIPGLKTAFEPASLEVSSLQESKLSLTMP
                     AGGEVKPFALEVKGAAADGKTNSLCLQVGPLKSGELAVVSQLAPPTKTRRNLEIILDA
                     SGSMKAVMAGKKTRWDVALETLDQVLNTLPDDFNVGLRMYGHREASTSPKTCTDTQLL
                     VPVRKLNRAAIMKSASAFKPKGETPLVYSSLQAPGDLKQLGGGTVILITDGEESCKGD
                     PVKAAEELKASGLDIRLNIVGFALKNPKTQQELAAFAQATGGLYYAANTGAALADAVT
                     VAAIEKFPYTVYDMAGKVVLASEAGSGSDPLPPGTYKVAVKAGARELVAPKVSVALGQ
                     SVTLTIAMKNGQLTLQ"
     gene            3208..4863
                     /locus_tag="PKK95_09655"
     CDS             3208..4863
                     /locus_tag="PKK95_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOC18523.1"
                     /translation="MSTSFSVSPAGERFPLPTPEDDARELARIEKLVAAARAEGQEIV
                     VVMGVGFVGAVMAAIVADSTGPDGTPGKFVIGCQRPSTRSYWKIPLLSRGESPVKAED
                     PEVEPMIRRTVLEKKTLVATHNSDCLRLADCVVVDVQCDFSKRDLGNMRSGAAEMSAL
                     EATMRTIGERIPASCLVLIETTVAPGTTEFVAWPIMKRAFAARGIAADPLLAHSFERV
                     MPGREYVRSIRDFWRVCSGCTAEAKARAAKFLGEILNTRDFPLTVMDRPIESETTKIV
                     ENSYRATILAFLNEWSLFAERNGVDLVKVIQAIKVRPTHDNIIFPGPGIGGYCLPKDG
                     GLGYWAYKHILGFEDQDALFKITPAAIDTNDTRALHVAELTRDALRNMGRYIAGADVL
                     LAGASYREDVGDTRYSGSELVVRKLTEMGAEMRVHDPYVDHWYELESQDTYPAPGHSW
                     ARFFRNQQGLTSLRVQHDLPKALAGAEALILAVRHAPYLVLEPDEVVRWAGGPLAVID
                     CFGILTDDRIRRYFELGCEVKALGRGHIQRIKEEVRGQRRVIV"
     gene            4870..4992
                     /locus_tag="PKK95_09660"
     CDS             4870..4992
                     /locus_tag="PKK95_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18524.1"
                     /translation="MLGVLEVPEVPEVLVLEVPEVLVPPESYRGSPFVTRCAFV"
     gene            complement(4950..6182)
                     /locus_tag="PKK95_09665"
     CDS             complement(4950..6182)
                     /locus_tag="PKK95_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC18525.1"
                     /translation="MGERGKVSLPGKLSLPGRVSRRLKWSAAGAAIAALLVAGGALAQ
                     RPGTVPPDEQGECPPGTTEIRPGRCAAPGLPPPSILDYRPRSTLVIAEHKVPRAKFPV
                     VDIHSHTGPTPETIDRLIAEMDALNVRVLVNLSGGGAAAVKQKVDYIRSSKHPDRFRV
                     FANVNFQGAGSPGWAEKQVADLEQAVRNGAIGLKVYKNLGLRVRKADGTRLKVDDPAL
                     KPVWEAAARLDIPVLIHTGEPQEFFSPLDYHNERWLELALFPDRRHNMPGEPSFEELM
                     KERDAMFAANPKTRFINAHFGWYGNDLARAAKLLDAAPNVVMDLSAVLYDFGRQPRAA
                     REFFIKYQDRVLFGKDSYAPSEFPYYWRVLETADEYFDYYRDYHAFWKLYGMDLPDEV
                     LRKVYHTNAQRVTKGLPR"
     gene            6393..7913
                     /gene="zwf"
                     /locus_tag="PKK95_09670"
     CDS             6393..7913
                     /gene="zwf"
                     /locus_tag="PKK95_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOC18526.1"
                     /translation="MAMAFEVREPGLRRGRPCEPCVMVIFGAAGDLARGDLVPSLYEL
                     HRKGLLPERFAAIGFSRSRWDSARYRDEMCRAVRESCGSSREWDGFASRLSFVSGDAS
                     APGDYAALARAIETVRSELGIPGNVFFHLAVPPNLFPAIVENLGASGLARADDGWRRL
                     VVEKPFGRDRESARDLDRRLREVFGEDQIYRIDHFLGKETVQNMLVFRFANPSFEPVW
                     NRNFIDHVQITVAEDIGIGTRANFYEQTGIVRDMVQNHVLHLLCLAALEPPVRFDADS
                     LRNETAKVLDAIVPPPLEDGATPVRGQYGEGVVDGGKVAGYRAEDGVPPGSTTPTYAA
                     MRLTIDNWRWADVPFYLRTGKRMPRKLTEVAIQFKPTPHAMFGQPQGRPRYRSVLAFE
                     LQPDEGIVQDVAAKQPGPDLEVQPVRMVFRYAEAFGVEEPPRAYAWLLLDVMQGDQTL
                     FARADWIDKAWQIVDPIVQRWESEPPARFPNYAAGTAGPAEATELLAREGRRWREL"
BASE COUNT         1147 a         2815 c         2796 g         1283 t
ORIGIN      
        1 acggccggcg gggccggcgg agcggcgtcg gccgcgggct gctcgtcttc ggccttgatc
       61 ttctcgatgt cggcgcgctt gccggcaatc acggcctgct cggcctcgat ctgctgctcg
      121 agcgtcgcga cccggtcggc cagcggctgc aggtcggcgg cgttcagccc acccgagcgg
      181 agcatcgcga tcgccttcgc gcccgcgtcg gccttgacct gggcgatctc ggcggtgaag
      241 ccggcgatct tccgctcgac ctccccgatc tcgcccttga tcttctgaat ccgcatgaac
      301 tggtcggcct cgcgcttggt cttgtccacc gcgtcgccga cggccttggt caccttgtcg
      361 aagaaaccca tgaacggtcc tcctgtccag cagggctgaa gccctgctct aacgctgctc
      421 acaggcgaga cgcctgtgcc actgtgccac tcacaggcga gacgcctgtg ctactggctg
      481 ctcacaggcg agacgcctgt gccactgtgc tactgcaggg tgagctggcc gttcttcatc
      541 gcgatcgtca gcgtcaccga ctggccgagc gcgacgctca ccttgggggc caccagctcg
      601 cgggcgcccg ccttcaccgc caccttgtag gtgccgggcg gcagggggtc gctgccgctc
      661 cccgcctcgc tcgcgagcac caccttcccg gccatgtcgt acacggtgta ggggaacttc
      721 tcgatcgccg cgaccgtcac cgcgtcggca agcgcggccc cggtgttggc cgcgtagtac
      781 aaccccccgg tcgcctgtgc gaaggcggcc agctcctgct gtgtcttcgg gttcttcagc
      841 gcgaagccga cgatgttgag gcggatgtcg aggcccgagg ccttcagctc ctccgcggcc
      901 ttcacgggat cgcccttgca gctctcctcg ccgtcggtga tgaggatcac cgtgccgccg
      961 cccagctgct tcaggtcgcc gggcgcctgc agcgacgagt agaccagcgg cgtctcgccc
     1021 ttgggcttga acgcgctggc gctcttcatg atcgcggcgc gattgagctt tcgcaccggg
     1081 acgagcagct gggtgtcggt gcaggtcttc ggcgaggtgg aggcctcgcg gtggccgtac
     1141 atcctcaggc cgacgttgaa gtcgtcgggc agcgtgttga gcacctggtc gagcgtctcg
     1201 agggcgacgt cccagcgcgt cttctttccc gccatcaccg ccttcatcga gcccgacgcg
     1261 tcgaggatga tctcgaggtt cctgcgcgtc ttggtgggcg gcgcgagctg gctgaccacc
     1321 gccagctcgc ccgacttcag aggtccaacc tggaggcaca gcgagttggt cttgccgtcg
     1381 gccgccgctc ctttgacctc cagcgcgaac ggtttcacct cgcccccggc aggcatcgtc
     1441 aaggagagct tcgattcctg gaggctcgac acctcgaggc ttgccggctc gaaggccgtc
     1501 ttcaggccgg ggattgcggg cgtggccgac gccgtgagcg acaccacccc cttgtagccg
     1561 aggttcgaga ccagcgagag cgggtactcg taggtcttcc cgggctgcac gccccgccag
     1621 ccgaggcgcg gctcggtggt gacgatgaag ttgccgaccc tgatcgagca cggcaggttg
     1681 tcggtgttga acccctgcga cagctggcgg gcgtggatga cgtccatcca ccgcgtgccg
     1741 accatgtcca cgtcgaggcc gtcccagatg atgaggccgc ggccgtggtg cgcgtacgcc
     1801 tggacgacgc cgaagatgcc gtcggcgttc ttcaccacca tgtggccgca ccagcccgga
     1861 tcccactcca tgatcgcgtt cgagtcgccc agctcgttgc ccggcggcgg ccagctcacc
     1921 cactcggcgg tgtccacgaa gccgggacgc gtcctgtagg tgtgcgccat ccagttgttc
     1981 tcgacgatgc gcagcacgga gcccggcttg ccgaaggctc ccgggttgtc cgacttgaag
     2041 cggtagggca gccagctgta gtcaggcgtg ctcccgcact tgtccgagtc gtggatgatc
     2101 agcttgtggc ccgcggcgac ccacgccgtc agcgccttgc ggaatcgcgg gctgagcggc
     2161 ttgaggttgc acgcctgctg catcaccacc gtgtccacgc cggcgagcat ccacggctgc
     2221 acgtggttga gtgcgacgag cctcgtcttg attcgcccga agatatccag ttccttccac
     2281 ttggacgggt acgtcttcgg gaagcggccg ctttcgatcc ccagcggcac gaacgagctg
     2341 ggcccgccca ggtagttggt catcagcagc agaacgttgc ggctctcgcc gtcgcccggg
     2401 tcgatgtcgg gatcggcatc gggcgagcag cccgccacgg gcgcgcgctg ggcggcggcc
     2461 gccttggctc cttcggccat gaagacgggc tcggcgatct cggggtggcg tgtgagaagc
     2521 ggcacgtagc ccggcgcctg gccctcgaac acctggatcc gcgcgatgcg gaaggcgtac
     2581 gggtttcccg cgaccttcgg gtcgcggtgg ttgttcagga agcggacctg gacgaagcgc
     2641 gcctcgaccg gctcgaaggt gagggttact tccgcgtgct tgaaggcgtt cggctcacgg
     2701 gcgaccgtcg cagcactcac cttcgtgaag ccctcgggcg cggccgcgtt cgtcgtccag
     2761 atctcgacgt cgcgcatccc gaggtcatgg ggcaggctct cgagcctgac ggccctgacg
     2821 agcaccgtct cgcgctcgaa gaacgagacg agggcctcga ccggcccttt gccctcaaca
     2881 gcgtggctcc agtcgccggc gaggatgccc tgcgcgtggt tgaggtcttt cgcgccgctc
     2941 aatgactcga gccggccgcc ccaggcgagg gcggcgacgt cgccatcgaa ggcggggacg
     3001 gcggactgct gcgccgccgc ggcgccgcgg gggggagggg cgcccggctt tggcgccgcc
     3061 tgttgcgcga ccgatacgga acccgccaga ccgagaagga ccaggctaac gaccagcact
     3121 ctcctcatgg gccgacgctc cccgggggcg ggtatcacgc gccaaggatt gtagctgagc
     3181 cagctccctg atctacactg tcccgccatg tccacctcct tttccgtttc ccccgcgggc
     3241 gagcgcttcc cgctccccac tcccgaggac gatgcgcgcg agctggcgcg gatcgagaag
     3301 ctggttgccg cggcccgcgc cgagggccag gagatcgtcg tcgtcatggg cgtcggcttc
     3361 gtgggcgccg tgatggccgc gatcgtggcc gactccaccg gtcccgacgg cacgccgggc
     3421 aagttcgtca tcgggtgcca gcgcccgagc acgcgcagct actggaagat cccgctgctg
     3481 tcgcggggcg agtcgccggt gaaggccgag gatcccgagg tcgagccgat gatccggcgc
     3541 acggtgctcg agaagaagac gctggtggcc acgcacaaca gcgactgcct gcggctggcc
     3601 gactgcgtgg tggtggacgt ccagtgcgac ttctcgaagc gcgacctcgg gaacatgcgg
     3661 agcggcgccg ccgagatgag cgcgctcgag gcgacgatga ggacgatcgg ggagcggatc
     3721 ccggcgtcgt gcctcgtgct gatcgagacc accgtggcgc cgggaaccac cgagttcgtg
     3781 gcgtggccga tcatgaagcg ggcgttcgcc gcccgcggca tcgccgccga cccgctgctg
     3841 gcgcacagct tcgagcgggt gatgcctggg cgcgagtacg tccgcagcat ccgcgacttc
     3901 tggcgggtct gctcgggctg cacggccgag gcaaaggcgc gcgcggcgaa gttcctcggc
     3961 gagatcctca acacccgcga cttcccgctc acggtgatgg accggccgat cgagtcggag
     4021 acgaccaaga tcgtcgagaa ctcgtaccgg gcgacgatcc tggcgttcct caacgagtgg
     4081 agcctgttcg ccgagcgcaa cggggtcgac ctggtgaagg tgatccaggc gatcaaggtg
     4141 cggccgaccc acgacaacat catcttcccg gggcccggca tcggcggcta ctgcctgccg
     4201 aaggacggcg gcctcggcta ctgggcctac aagcacatcc tcggcttcga ggaccaggac
     4261 gccctcttca agatcacgcc ggcggcgatc gacaccaacg acacccgcgc gctgcacgtc
     4321 gccgagctga cgcgcgacgc gctgcggaac atggggcgct acatcgccgg ggccgacgtg
     4381 ctgctggcgg gcgccagcta ccgcgaggac gtcggcgaca cgcgctacag cgggtcggag
     4441 ctggtcgtgc ggaagctgac cgagatgggc gccgagatgc gggtgcacga cccgtacgtg
     4501 gaccattggt acgagctgga gagccaggac acctacccgg cgccgggcca ctcgtgggcg
     4561 cgcttcttcc gcaaccagca ggggctcacc tcgctgcgcg tgcagcacga cctgccgaag
     4621 gcgctcgccg gggccgaggc gctgatcctc gcggtgcgcc acgcgccgta cctggtgctc
     4681 gagcccgacg aggtggtgcg ctgggccggc gggccgctgg cggtgatcga ctgcttcggc
     4741 atcctgaccg acgaccgcat ccgccgctac ttcgagctgg ggtgcgaggt gaaggcgctc
     4801 ggccgcggcc acatccagcg gatcaaggag gaggtgaggg ggcagaggag agtgatcgtg
     4861 tagcgtgtcg tgctgggggt gctggaggtg ccggaggtgc cggaggtgct ggtgctggag
     4921 gtgccggagg tgctggtgcc ccccgaatcc taccgcggca gccccttcgt cacgcgctgc
     4981 gcgttcgtgt gatacacctt ccgcagcacc tcgtcgggga ggtccatccc gtacagcttc
     5041 cagaacgcgt ggtagtcgcg gtagtagtcg aagtactcgt ccgcggtctc gagcacgcgc
     5101 cagtaatacg ggaactcgga cggcgcgtac gagtccttcc cgaacagcac gcggtcctgg
     5161 tacttgatga agaactcgcg ggcggcgcgc ggctggcggc cgaagtcgta cagcacggcc
     5221 gagagatcca tcaccacgtt cggggcggcg tcgagcagct tcgcggcccg cgcgaggtcg
     5281 ttgccgtacc agccgaagtg cgcgttgatg aaccgcgtct tcgggttcgc ggcgaacatc
     5341 gcgtcgcgct ccttcatcag ctcctcgaag ctgggctcgc cgggcatgtt gtggcgccga
     5401 tcggggaaga gcgccagttc gagccaccgc tcgttgtggt agtcgagcgg cgagaagaac
     5461 tcctgcggct cgccggtgtg gatcaggacg gggatgtcga ggcgcgcggc cgcctcccag
     5521 accggcttca gcgccggatc gtccaccttc aggcgtgtgc cgtcggcctt gcgcacgcgc
     5581 agcccgaggt tcttgtacac cttcaggccg atcgccccgt tgcgcaccgc ctgctcgaga
     5641 tcggccacct gcttctcggc ccagcccggg ctgccggcgc cctggaagtt cacgttggcg
     5701 aagacgcgga agcggtcggg gtgcttgctc gatcggatgt agtcgacctt ctgcttcacg
     5761 gcggcggcgc cgccgccgct caggttcacc agcacgcgca cgttcagcgc gtccatctcg
     5821 gcaatcagcc ggtcgatcgt ttccggcgtc gggccggtgt ggctgtggat gtcgacgacg
     5881 gggaacttcg cgcgcgggac cttgtgctcg gcgatcacca gcgtggagcg ggggcggtag
     5941 tcgaggatgc tggggggcgg cagcccgggc gccgcgcacc gcccggggcg gatctcggtc
     6001 gtccccggcg ggcactctcc ctgctcgtcg ggcggtacgg tgccgggccg ctgcgcaagc
     6061 gcgccgcctg cgaccagcag cgctgcgatt gccgccccgg cggccgacca cttcagcctg
     6121 cgcgacaccc ggcccggcag cgacagcttc cccggcagcg acaccttccc ccgctcgccc
     6181 atgccctctc ctctgttgac agccctgctc ccatgatagc cgctcggggc cagccgggcc
     6241 ccgcggagtt cccgcgccgc cgccgccgac cacccaagcg agccgcccat cgtcgcggat
     6301 gcatgtggta gccccgacag cgagaccggt cggcccggcg ggcggacgcg gtaagatcga
     6361 gccggggcaa tccaagcgag gcggaggaag agatggcgat ggctttcgag gtgcgcgagc
     6421 ccgggctccg ccgcggccgg ccgtgcgagc cgtgcgtgat ggtgatcttc ggcgccgccg
     6481 gcgacctcgc ccgcggcgac ctcgttccct cgctctacga gctgcaccgc aagggcctgc
     6541 tccccgagcg cttcgccgcc atcggcttct cgcgcagccg ctgggactcg gcgcgctacc
     6601 gcgacgagat gtgccgcgcg gtccgcgaga gctgcggcag ctcccgcgag tgggatggct
     6661 tcgcctcgcg cctgagcttc gtcagcggcg acgcctccgc gcccggcgac tacgcggcgc
     6721 tcgcccgcgc catcgagacg gtgcggtccg agctgggcat ccccggcaac gtcttcttcc
     6781 acctcgcggt gccgccaaac ctcttccccg ccatcgtgga gaacctcggc gcctcgggcc
     6841 tggcccgcgc cgatgacggc tggcggcgcc tggtcgtcga gaagccgttc gggcgcgacc
     6901 gcgagagcgc ccgcgacctc gaccgccggc tgcgcgaggt gttcggcgag gaccagatct
     6961 accggatcga tcacttcctg gggaaggaga cggtgcagaa catgctcgtc ttccgcttcg
     7021 ccaaccccag cttcgagccg gtctggaacc gaaacttcat cgaccacgtc cagatcaccg
     7081 tcgccgagga catcggcatc ggcacccgcg ccaacttcta cgagcagacg ggcatcgttc
     7141 gcgacatggt gcagaaccac gtgctgcacc tgctctgcct cgccgccctc gagccgccgg
     7201 tccgcttcga cgccgactcg ctgcgcaacg agaccgccaa ggtgctcgac gcgatcgtcc
     7261 cgcccccgct cgaggacggc gcgacgccgg tgaggggcca gtacggcgag ggggtggtgg
     7321 acggcgggaa ggtggcgggc taccgcgcgg aggacggcgt gccgcccggc tcgaccaccc
     7381 ccacctacgc cgccatgcgt ctcaccatcg acaactggcg ctgggccgac gtcccgttct
     7441 acctgcgcac cggcaagcgc atgccgcgca agctgaccga ggtcgccatc cagttcaagc
     7501 cgacgccgca cgcgatgttc gggcagccgc aggggcggcc gcgctaccgc agcgtgctcg
     7561 cgttcgagct gcagccggac gaggggatcg tgcaggacgt cgccgcgaag cagccggggc
     7621 ccgacctcga ggtgcagccc gtgcgcatgg tcttccgcta cgccgaggcg ttcggcgtcg
     7681 aggagccgcc ccgcgcctac gcgtggctcc tcctcgacgt gatgcagggc gaccagacgc
     7741 tcttcgcccg cgccgactgg atcgacaagg cctggcagat cgtcgacccg atcgtccagc
     7801 gctgggagtc ggagccccct gcccgctttc cgaactacgc ggccggcacc gccggccccg
     7861 ccgaggccac cgagcttttg gcgcgcgaag ggcgaaggtg gagggagctg tagcgcgccg
     7921 ctacggtttc ccgcccagcg agtggttgtt tgcgacgatg gcgaggtagg cgccgttgag
     7981 gacggccatc agcaccaccg ccgccgcccg gttcctcttc cgcaaccgct ccgccaggta
     8041 g
//
LOCUS       DAOPQX010000160         8001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10090_length_8001_cov_4.769192, whole genome shotgun sequence.
ACCESSION   DAOPQX010000160 DAOPQX010000000
VERSION     DAOPQX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10090_length_8001_cov_4.769192"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKK95_09675"
     CDS             <1..416
                     /locus_tag="PKK95_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18527.1"
                     /translation="AHRRLAAACPTFLGNVEARDVYAGEADVIVCDGFTGNIVLKISE
                     GLVEIVGEVFRERLLAEGRATSTADGDALPRRLDWSEYGGVPLLGVAGLALVGHGRSS
                     ARAVRNGIAMAYRHAAAGILSRIQLAIAPTGATSS"
     gene            413..1339
                     /gene="fabD"
                     /locus_tag="PKK95_09680"
     CDS             413..1339
                     /gene="fabD"
                     /locus_tag="PKK95_09680"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOC18528.1"
                     /translation="MIAFIFPGQGSQAVGMGRALAESFDVCRETFAEADAALGESLST
                     LCFEGPEAALRLTENTQPAILTVSVAAYRLLESKGYRPDLVAGHSLGEYSAHVAAGTL
                     AFADAVRIVRRRGRYMQEAVPVGDGAMAAVIGLDAGAVERACLEAAQGEVVSPANLNM
                     PGQVVIAGGRDAVARASEIAKGMGAKRVVPLAVSAPFHCALMKPAELRLEPELRALPA
                     RDPRVPVVANVDAEPKRDAASAIDALVRQVSSPVRWEDVVRRLASEGVRKYVEVGPGT
                     VLTGLGRKIHRDAAYASVEGPEGLEQVAALFT"
     gene            1480..2223
                     /gene="fabG"
                     /locus_tag="PKK95_09685"
     CDS             1480..2223
                     /gene="fabG"
                     /locus_tag="PKK95_09685"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOC18529.1"
                     /translation="MFELTGKVAIVTGGSRGIGLAIGETLARQGAHVVLADVLGEEAA
                     RAAAAIGERGGLASAVALDVTRPEMVQAAVDQVLERQGRIDVLVNNAGIARDQLLMRM
                     KREEWDAVVAVNLTGAFTCAQAVLRPMMRQRSGRIISISSVVGQMGNAGQSNYAASKA
                     GLIGFTKALAREVAARSVTVNAVAPGLIDTDMTRALSEGVRSEWAAKVPLGRLGSPDD
                     VAWTVAFLASDEASYVTGQVIAVNGGMYT"
     gene            2232..2477
                     /locus_tag="PKK95_09690"
     CDS             2232..2477
                     /locus_tag="PKK95_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOC18530.1"
                     /translation="MAAVADKVKTIIVDQLGVDEEEVTPDASFVDDLGADSLDTVELV
                     MAFEEEFGIEIPDEDAEKITRVKEAVEYIEGHAKNKK"
     gene            2561..3799
                     /gene="fabF"
                     /locus_tag="PKK95_09695"
     CDS             2561..3799
                     /gene="fabF"
                     /locus_tag="PKK95_09695"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOC18531.1"
                     /translation="MSRRVVVTGIGLVSPLGIGTEANWQGLQAGRSGVARITRFDTSN
                     FAAQIAGEVKGFDPLQFFEKKEVKKVDVFIQYAVAAAQFAVDDSGLKIGGIGDRVGVA
                     IASGIGGFATIEREHQALLEGGPRRISPFFIPAAIINLAAGHVSIRFGAKGPNMASCT
                     ACSASAHAVGDAYEIIRRGDADAMIAGGSEAAITPMGVGGFAAMRALSTRNDAPERAS
                     RPFDKDRDGFVIGEGSGVMVLEEREAALARGARIYAEVVGYGASADAFHITAPSEDGD
                     GAVRSMRAALRSAGVDGSVVAYINAHGTSTPLNDRLETIAIKRCFGEHAHKLAVSSTK
                     SMTGHLLGAAGGLEGGITALAVYRQEMPPTINLENPDPDCDLDYVPGTARKAEIPYAL
                     SNSFGFGGTNASLLFKRADL"
     gene            3878..4639
                     /locus_tag="PKK95_09700"
     CDS             3878..4639
                     /locus_tag="PKK95_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOC18532.1"
                     /translation="MKIAVCLKQVPARDAQPRVNESKTWLREQDATFELNEPDAYALE
                     EGFRLRERHGGEVVVCSAGPARVAQALREALARGADRAIHVEDDRLASADAFAVAEAL
                     AGAIEGERFDLVLTGLQSDDQGFAQTGVVLAERLGIPHATIIMEVAVQGDRLRVKREL
                     EGGWFQWVEMPLPALLTIQSGINQLRYATLKGIIAAKKKEIRKVAPAGVASRLRIVRL
                     RAPEKTKQTQIIPGPAADAAKELVRRLRDEARAIE"
     gene            4647..5630
                     /locus_tag="PKK95_09705"
     CDS             4647..5630
                     /locus_tag="PKK95_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOC18533.1"
                     /translation="MILAVAEQREGTLNRASWEAIAAAQRLAAQTGLGPVRIAIAGGE
                     VAASAASLAEAECAEVVALEAAALGAYTADGFTAALARLLEAEAPAFALFAHTYQARD
                     FVPKLAARLGWPLVTDVTGYGEPFEGRRTFTRLVFQGKLAADVVAEGDGPCLVTVQAG
                     AFPADGVARGASPAPVRRVEATADSSAIRQKPEPPFQEAKQAVDLSQASRIVAVGRGI
                     KEQSKLALVEELARALGAEIAASRPICDAGWLPLERQVGSSGQTVSPKLYLALGISGA
                     IQHAVGMKGSRTIVAINKDAEAPIFELADYGIVGDLFEVVPELVKELGKSK"
     gene            5725..7533
                     /locus_tag="PKK95_09710"
     CDS             5725..7533
                     /locus_tag="PKK95_09710"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOC18534.1"
                     /translation="METLILWVLVLVFGGLFAWQVATRVRLIRRAPGRLGVADAGAAA
                     GRLLREVVFQSKVIAERPLVGLAHALVFWGFVAFAGYTSTEFLAGLGLVDLTGTAAFR
                     FYSLALVPFAIAVLAGILVLLVRRVVFRPEALGKLSGESVVIGLFIAALMVTFLLDFR
                     LAGESLGGRVNWWVHALVILVFLVLIPGSKHFHLLLSPITIALESPVLGEVRNLDFEK
                     EEIGYETVAHLERKMVLDAFTCVECGRCQMNCPAYRTGKLLNPKKLILDNEDALLAGK
                     LDAPLKSIYDENVLWQCTTCGACEAQCPVGIEHLPLIINARRGLVSNGEAPDALGAMY
                     NNLERRGNIWGLPQDQRQKFVAASGLETFDASRHEYLMWLGCAGSYDADFQKSLRSLF
                     DLLRRLEVTFGVLEKERCTGDPAKRTGNEYVYQELANANIEEFRAAGVRKILTSCPHC
                     VKTLGPDYRLLGFEAEVVHSSVLLSRTLPGQLPASDTVVLHDPCYLARYAGHVDEPRD
                     LLRRVGATVREPVRNRHNPFCCGAGGGLLFEEREEGQRISQTRFEQLQATGAPTIVTA
                     CPFCSIMLKGAQASSNATTQVVDITSYVDGRLKSTS"
     gene            7715..>8001
                     /locus_tag="PKK95_09715"
     CDS             7715..>8001
                     /locus_tag="PKK95_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18535.1"
                     /translation="MTTGTGPRVAGKAPGVPARDGPPDWRASRIKRVEAAAIGALGYP
                     LAAALGRTWRWEARGTEHLDAVRASGRQPILALWHGRILPAVLYFRGRGIVA"
BASE COUNT         1134 a         2788 c         2935 g         1144 t
ORIGIN      
        1 aggcgcaccg ccgcctggcc gccgcctgcc cgaccttcct cggtaacgtc gaggcgcgcg
       61 acgtctacgc cggcgaggcc gacgtcatcg tctgcgacgg cttcacgggc aacatcgtgc
      121 tcaagatcag cgaggggctc gtggagatcg tcggcgaggt gttccgcgag cgcctgctcg
      181 ctgaggggcg cgcgacctcc accgccgacg gcgacgccct tccacgccgc ctcgactggt
      241 ccgagtacgg gggcgtcccg ctgctcggcg tggcggggct cgcgctcgtg ggccacggcc
      301 gctcgtcggc ccgcgccgtc cgcaacggga ttgccatggc ctaccggcac gccgcggccg
      361 gcatcctctc gcgcatccaa cttgccatcg cccccactgg agcgacatcg tcgtgattgc
      421 cttcatcttc cccgggcagg gttcccaggc ggtcggcatg ggccgggcgc tggccgagtc
      481 gttcgacgtg tgccgcgaga ccttcgccga ggcggacgcg gcgctgggcg agagcctcag
      541 caccctctgc ttcgaagggc ccgaggcggc cctgcgcctg accgagaaca cgcagcccgc
      601 gatcctcacc gtgagcgtgg cggcgtaccg gctgctcgag tcgaaggggt accggcccga
      661 cctggtggcg ggtcacagcc tgggggagta ctcggcgcac gtggcggctg gcacgctggc
      721 gttcgccgac gcggtccgga tcgtgcggcg ccgcgggcgc tacatgcagg aggcggtgcc
      781 ggtgggggac ggcgcgatgg ccgcggtgat cggcctcgac gccggcgcgg tcgagcgggc
      841 gtgcctcgag gcggcgcagg gagaggtcgt gagcccggcg aacctgaaca tgccggggca
      901 ggtggtgatt gccggcggcc gcgacgcggt ggcgcgtgcg tcggagatcg ccaaggggat
      961 gggcgccaag cgggtcgtgc cgctcgcggt gagcgccccc ttccactgcg cgctgatgaa
     1021 gccggcggag ctgcgcctcg agcccgagct gcgcgcgctt cccgcgcgcg acccgcgggt
     1081 gccggtggtc gccaacgtgg acgccgagcc gaagcgcgac gccgcctcgg cgatcgacgc
     1141 gctggtgcgc caggtttcct ccccggtccg ctgggaggac gtggtgcgcc ggcttgcatc
     1201 ggagggcgtc cgcaagtatg ttgaagtggg cccgggcacc gtgctgaccg ggctgggacg
     1261 caagatccac cgcgacgcgg cgtacgcgag cgtcgagggc cccgagggcc tcgagcaggt
     1321 tgcggcgttg ttcacgtagg ccacccgttc ttcgccctgc cccgccatcg cgccgagagc
     1381 tgaagatgcg cgcgcccggc cagccgcttc cgccgcgcgt gatcgaaacg gccgggaagc
     1441 gtgtatgatg tgtcgccgcg ccgggagggg actgctgcga tgttcgaact gacggggaag
     1501 gtcgcaatcg tgacgggcgg gtcgcggggc atcggcctcg cgatcgggga gacgctcgcg
     1561 cgccaggggg cgcacgtcgt gctggcggac gttctcggcg aagaggccgc gcgcgccgcg
     1621 gcggcaatcg gcgagcgcgg ggggctggcg agcgcggtgg cgctcgacgt gacgcggccc
     1681 gagatggtcc aggcggcggt cgaccaggtg ctcgagcggc agggccggat cgacgtcctc
     1741 gtcaacaacg cgggaatcgc ccgcgaccag ctcctcatgc ggatgaagcg cgaggagtgg
     1801 gacgcggtcg tagccgtcaa cctgaccggc gcgttcacct gcgcgcaggc ggtgcttcgg
     1861 ccgatgatgc gccagcggtc ggggcggatc atcagcatca gctcggtcgt cggccagatg
     1921 ggcaacgccg ggcagtcgaa ctacgcggcg tcgaaggcgg ggctgatcgg gttcaccaag
     1981 gcgctcgcgc gcgaggtggc ggcccgcagc gtcaccgtga acgcggtggc ccccggcctc
     2041 atcgacaccg acatgacgcg cgcgctcagc gagggcgtgc gcagcgagtg ggccgccaag
     2101 gtgccgctgg gccggctcgg gagccccgac gacgtggcgt ggacggtcgc cttcctggcg
     2161 tccgacgaag cgtcctatgt caccgggcag gtgatcgccg tcaacggcgg gatgtacacg
     2221 taggaggacg aatggccgcg gtagcagaca aggtcaagac gatcatcgtg gatcagctcg
     2281 gtgtggacga ggaggaggtc acccccgacg cctcgttcgt cgacgacctc ggcgccgact
     2341 cgctcgacac cgtggagctg gtgatggcgt tcgaggagga gttcggcatc gagatccccg
     2401 acgaggacgc cgagaagatc acccgcgtga aggaagccgt cgagtacatc gaagggcacg
     2461 ccaagaacaa gaagtagcgg cccggcagcc gggcgccacg cgcggagcgc cggcagcgcc
     2521 gcgcccgtgg tgtgcgcccg ggggcgcaac gggagggagc ttgagcaggc gagtcgtcgt
     2581 caccggcatc ggcctcgtgt cgcccctggg gatcggcacc gaggccaact ggcagggcct
     2641 ccaggcgggt cgtagcggcg tcgcgcgcat cacgcgcttc gacacctcga acttcgcggc
     2701 gcagattgcc ggggaagtga aggggttcga tccgctgcag ttcttcgaaa agaaggaagt
     2761 caagaaggtc gacgtcttca tccagtacgc cgtggcggcc gcgcagttcg ccgtcgacga
     2821 ctcggggctg aagatcggcg gcatcggcga ccgcgtcggg gtggcgatcg cctccggcat
     2881 cggcgggttc gccacgatcg agcgcgagca ccaggcgctg ctcgagggcg ggccccgcag
     2941 gatctccccc ttcttcatcc ccgccgccat catcaacctg gccgcggggc acgtgtccat
     3001 ccgcttcggg gccaagggcc ccaacatggc gagctgcacg gcctgctcgg cctcggcgca
     3061 cgcggtcggc gacgcctacg agatcatccg ccgcggcgac gccgacgcga tgatcgcggg
     3121 cggctcggag gcggcgatca ccccgatggg ggtcggcggc ttcgcggcca tgcgcgccct
     3181 ctcgacccgc aacgacgccc ccgagcgcgc cagccggccg ttcgacaagg accgcgacgg
     3241 cttcgtgatc ggcgaggggt cgggcgtgat ggtgctcgag gagcgcgagg cggcgctcgc
     3301 acgcggcgcc cgcatctacg ccgaggtggt cggctacggc gcctcggccg acgcgttcca
     3361 catcacggcc ccgtcggagg acggcgacgg cgccgtccgg tcgatgcggg ccgccttgag
     3421 gagcgccggc gtggacggat cggtcgtcgc ctacatcaac gcgcacggca cctcgacgcc
     3481 gctcaacgac cgcctcgaga cgatcgccat caagcggtgc ttcggcgagc acgcccacaa
     3541 gctggccgtc tcgtccacca agtcgatgac cgggcacctg ctcggcgcgg caggcggcct
     3601 cgaggggggc atcacggcgc tggcggtcta ccggcaggaa atgccgccga ccatcaacct
     3661 ggagaacccc gaccccgact gcgacctgga ctacgtgccg gggaccgccc gcaaggcgga
     3721 gatcccctac gcgctgtcca actcgttcgg cttcggcggc accaacgcct ccctcctctt
     3781 caagcgcgcc gacctgtagc ggcaaggaac cgtccgggac gaatccgcgg aaattcgtcc
     3841 cggacccctg atccgctatg ctagtcggga tacctctatg aagatcgctg tctgcctcaa
     3901 gcaggtccct gcccgcgacg cccagccgcg ggtcaacgag tcgaagacct ggctccgcga
     3961 gcaggacgcc accttcgagc tgaacgagcc cgacgcctac gccctcgagg agggcttccg
     4021 gctgcgcgag cggcacggcg gcgaggtggt ggtctgctcg gccggccccg cccgcgtggc
     4081 gcaggcgctg cgcgaggcgc tcgcccgcgg cgccgaccgt gcgatccacg tcgaggacga
     4141 tcgcctggcc tcggccgacg ccttcgcggt ggctgaggcg ctcgccggcg ccatcgaggg
     4201 cgagcggttc gacctggtgc tgaccggcct ccagtccgac gaccagggct tcgcccagac
     4261 cggcgtcgtg ctggccgagc gcctcggcat cccgcacgcg accatcatca tggaggtcgc
     4321 cgtgcagggg gaccgcctcc gcgtcaagcg cgagctcgag ggcgggtggt tccagtgggt
     4381 cgagatgccg ctcccggcgc tgctcaccat ccagagcggc atcaaccagc tgcgctacgc
     4441 gaccctgaag ggaatcatcg ccgcgaagaa gaaggagatc cggaaggttg cccctgccgg
     4501 ggtggcctcg cggctgcgga tcgtccgtct tcgcgcgccc gagaagacca agcagaccca
     4561 gatcattccc ggcccggccg ccgacgccgc caaggaactg gtgcggcgcc tgcgcgacga
     4621 ggcccgcgcg atcgagtagg tgcagcatga tcctcgccgt tgccgaacag cgtgagggaa
     4681 cgctcaaccg ggcctcgtgg gaggccatcg ccgccgcgca gcgtctggcg gcccagacgg
     4741 gcctcgggcc cgtgcggatc gcgatcgcgg gcggcgaggt ggccgcctcg gccgcgagcc
     4801 tggccgaggc cgagtgcgcg gaggtggtgg ccctcgaggc ggccgccctt ggcgcctaca
     4861 ccgccgacgg gttcacggcg gcgctcgccc ggctgctcga ggccgaggct cccgccttcg
     4921 ccctgttcgc gcacacctac caggcgcgcg acttcgtccc gaagctggcc gcccgcctcg
     4981 gctggccgct cgtcaccgac gtgaccggct acggcgaacc gttcgagggg cgtcggacct
     5041 tcacccgcct cgtgttccag ggcaagctgg ccgccgacgt cgtggccgag ggcgacggcc
     5101 cctgcctggt gacggtgcag gccggggcgt tcccggccga cggcgtcgcg cgaggggcgt
     5161 cgcccgcccc ggtgcgccgg gtcgaggcga ccgcagattc gtcggcgatc cggcagaagc
     5221 ccgagccccc gttccaggag gcgaagcagg cggtcgacct ctcgcaggct tcacgcatcg
     5281 ttgccgtcgg gcgcggcatc aaggagcagt cgaagctggc gctggtcgag gagctggccc
     5341 gcgcgctcgg ggccgagatc gcggcctcgc ggcccatttg cgacgcgggg tggctcccgc
     5401 tcgagcggca ggtcggcagc tcgggccaga cggtgtcgcc gaagctgtac ctcgccctcg
     5461 ggatctcggg cgccatccag cacgccgtcg ggatgaaggg atcgcggacg atcgtcgcga
     5521 tcaacaagga cgccgaggcg ccgatcttcg agctggccga ctacggcatc gtcggcgacc
     5581 tgttcgaggt cgtgccggag ctggtcaagg agctggggaa gagcaagtag ccggcggcta
     5641 gtggatagtg gctggtggct ggcgggggtg acagacagta ccggccggcc gtgaagcgtc
     5701 tcgcgccccc gtgaggaccc tctgttggaa accctgatcc tctgggtgct cgtcctcgta
     5761 ttcggcggcc tgttcgcgtg gcaggtggcg actcgcgtcc gcctgatccg ccgggcgccg
     5821 ggacgcctcg gcgtggcgga tgccggcgcg gcggccgggc ggctgctgcg cgaggtggtg
     5881 ttccagtcga aggtgatcgc ggagcggccg ctggtggggc tggcgcacgc gctcgtcttc
     5941 tggggcttcg tggccttcgc cggctacacc agcacggagt tcctcgccgg gctgggcctg
     6001 gtcgacctca ccggcaccgc cgccttccgc ttctactcgc tcgcgctggt tcccttcgcc
     6061 atcgccgtgc tcgccgggat cctcgtcctg ctggtgcgcc gggtggtctt ccgtcccgag
     6121 gcgcttggca agctgtcggg cgagtcggtg gtgatcggcc tgttcatcgc ggcgctgatg
     6181 gtcaccttcc tgctcgactt ccgcctggca ggggagagcc tcggcgggcg ggtgaactgg
     6241 tgggtgcacg cgctcgtcat cctcgtcttc ctggtgctca tccccggctc gaagcacttc
     6301 cacctgctgc tctcgccgat cacgatcgcg ctcgaatcgc cggtgctcgg cgaggtgcgg
     6361 aacctcgact tcgagaagga ggagatcggc tacgagacgg tggcgcacct cgagcgcaag
     6421 atggtgctcg acgccttcac gtgcgtcgag tgcgggcgct gccagatgaa ctgccccgcc
     6481 tatcgcacgg gcaagctgct gaacccgaag aagctgatcc tcgacaacga ggacgcgctg
     6541 ctggcgggca agctggacgc gccgctgaag agcatctacg acgagaacgt gctgtggcag
     6601 tgcaccacgt gcggcgcgtg cgaggcgcag tgcccggtgg ggatcgagca cctgccgctc
     6661 atcatcaacg cgcgccgcgg cctggtgtcg aacggggagg cgcccgacgc gctgggcgcg
     6721 atgtacaaca acctggagcg ccgcgggaac atctgggggc tgccgcagga ccagcgccag
     6781 aagttcgtgg ccgcctcggg cctcgagacc ttcgacgcct cgcgccacga gtacctgatg
     6841 tggctcggct gcgcggggtc ctacgacgcc gatttccaga agtcgctgcg gtcgctcttc
     6901 gacctgctgc gccggctgga ggtgaccttc ggcgtgctcg agaaggagcg ctgcacgggt
     6961 gatcccgcca agcgcaccgg caacgagtac gtgtaccagg agctggccaa cgcgaacatc
     7021 gaggagttcc gggcggcagg cgtcaggaag atcctgacct cgtgcccgca ctgcgtgaag
     7081 acgctcggcc ccgactaccg gctgctcggc ttcgaggccg aggtcgtgca ctcctcggtg
     7141 ctgctgtcgc gcacccttcc gggacagctg cccgcgtccg acaccgtggt gctgcacgac
     7201 ccctgctacc tggcccggta cgccgggcac gtcgacgagc cgcgcgacct gctgcggcgc
     7261 gtgggggcga ccgtgcgcga gcccgtgcgc aaccgccaca accccttctg ctgcggagcc
     7321 ggcggcggcc tgctgttcga ggagcgcgag gagggacagc ggatcagcca gacgcggttc
     7381 gagcagctgc aggccaccgg cgccccgacg atcgtcaccg cctgcccctt ctgctcgatc
     7441 atgctcaagg gggcccaggc gagcagcaac gccaccacgc aggtggtgga catcacctcg
     7501 tacgtggacg gccgcttgaa gagcacctcg tagcggcttg tccacgtacc gaaccacaaa
     7561 gatcacaaag atcacaaaga ctgcgaagct cgctgcgcgg cggccttcgg cgaggcgcat
     7621 cgcgcctcgc cgaaggcggc cgcgttccgt cgggccgccc cgccgcacgt ccctttgtgt
     7681 cctttgcgtc ctttgtggtt aacttccgtg cggtgtgacc actggcacag gcccgcgagt
     7741 ggcgggaaag gctcccggcg ttccggcccg tgacggcccg cccgactggc gggcctcgcg
     7801 tatcaagcgc gtcgaggcgg ccgcgatcgg cgcccttgga tacccgctcg ccgcggccct
     7861 cggccggacc tggcgctggg aggcgcgggg caccgaacac ctcgacgcgg tgcgcgcctc
     7921 ggggcgccag ccgatcctcg ccctctggca cggccgcatc ctgccggccg tcctctactt
     7981 ccgcggccgc ggcatcgtcg c
//
LOCUS       DAOPQX010000161         8001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10091_length_8001_cov_4.434432, whole genome shotgun sequence.
ACCESSION   DAOPQX010000161 DAOPQX010000000
VERSION     DAOPQX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10091_length_8001_cov_4.434432"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="PKK95_09720"
     CDS             <1..1147
                     /locus_tag="PKK95_09720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOC18536.1"
                     /translation="LEVVLRESRLREDLVPKVVELAASVFDPRRLRADNPFKLERTLE
                     GLLRSFEYEIDTDSFLRIVGLSDEQPAELRAEIVPYKKERVVTTASGLINRDATSLFA
                     AMELAGEGPQLSIELADVFGGEIDFNSELQPGDTFRVAFEKVYREGTFSGYGGVLAAE
                     FVNVGHSFKALRYTVPGGKPAYYDEQGRSLKRFFLKSPLKFEASISSRFTHSRRHPIL
                     RIVRPHLGVDYRAPIGASVVAVANGTVVSAGWSGGAGRMVTLRHPGGWETLYMHLSSI
                     AVRRGQHVSQGQLIGRVGSSGLSTGPHLDYRVRQNGRYLNPLVVHRSLPPGEPIPAAH
                     LAAFREEFERSWTLLSATEATPVEGAPGAVKAASEPAADPPLPKNVR"
     gene            1234..>2130
                     /locus_tag="PKK95_09725"
     CDS             1234..>2130
                     /locus_tag="PKK95_09725"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOC18537.1"
                     /translation="MSMIRPFTALRPQPDNVDRVSAVPYDVVNTEEARAMAGDNALSF
                     LHVSRAEIDLPPSTDPYSDAVYAKALENFQRLRAEAPLVVEEAPSLYFYRLRMGTHEQ
                     TGLAACYSVDEYDAGTIKKHEKTRRDKEDDRTRHIGTLRAQTGPVFLTYRASAAVDAI
                     ASRVTAGAPLFDFESADRVRHTVWRASGADVEALVQAFAELPCLYIADGHHRAASAAR
                     TRKHLAGLEPGVAHPEADWMLAVAFPDNQTAIMPYNRVVKDLGTYTPETLLAGLREKL
                     RVVEGAASPAAKGEVSMYLAGRW"
     assembly_gap    2131..2140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2141..2466
                     /locus_tag="PKK95_09730"
     CDS             <2141..2466
                     /locus_tag="PKK95_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18538.1"
                     /translation="TIALQPPEGTGDVGSTLDVSLLQAQVLTPFLGIGDPRTDKRIDF
                     VGGIRGTGELERLVREGKAAVAFSMFPVGVDDLMRIADAGEIMPPKSTWFEPKLRDGL
                     LSHLI"
     gene            2506..3777
                     /locus_tag="PKK95_09735"
     CDS             2506..3777
                     /locus_tag="PKK95_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC18539.1"
                     /translation="MKVLVADKFEKSGIEGLKAAGCEVIFEPDLKDAALAEAIGRTGA
                     DVLVVRSTVVTEPMMNAGRLALIVRAGAGYNTIDVKAASRRGIYVSNCPGKNSVAVAE
                     LAFGLMIALDRRIPDNVAELQRGKWNKKEYSKARGLAGSTLGLLGFGKIGQEMAKRAM
                     AFHMNLIIWSELGVRQDRDAIPVDLPLLLEMRRGHDASGLIQERISVVNSPAEVAEKC
                     DVLSIHLASSPLTKNIVNAEVIGKLKPGAYVINTARADVVDYDALARAVVEKGVRVGL
                     DVYPGEPASPTADYAHPLLGLPGVYGTHHIGASTNQAQEAIAEETVRIVTAYKETGRV
                     PNVVNLAKKTPATHSLIVRHRDQPGVLACLFAKLAGAGINAHEAENIVFETAEAAIAR
                     INVDREPSAALIESFRNDCPAVYEVKIVALA"
     gene            3896..5002
                     /gene="serC"
                     /locus_tag="PKK95_09740"
     CDS             3896..5002
                     /gene="serC"
                     /locus_tag="PKK95_09740"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOC18540.1"
                     /translation="MVTTTRVFNFAAGPAVLPLPALEEAQRDLVALPNVGMSVLEISH
                     RSKTFEAIIQECEANVRKLANVPENYHVLFLQGGATTQFSMVPMNLLPPGAWADYIVT
                     GSWSSKAVKEAKKVGAVNVAFDGKGENFVRVPKAGELKLSKDPAYVHVTTNETIHGVE
                     FHELPDVGERVLVADTSSHMFSRPIDVTKYGLIYAGVQKNLGPAGATMVIVRDDLVKK
                     AAEYKGYANLPVMLQYGTHVPEKSLYNTPPVFTIYMVGLVMKWLVGLGGLEAMERINI
                     RKAEKLYAEIDRSGFYRGHAAKDSRSRMNVTFRLPSEELEAKFVKESKAAGLDGLKGH
                     RSVGGLRASIYNAFPEEGVDALVAFMQEFEKKNG"
     gene            complement(5077..6408)
                     /locus_tag="PKK95_09745"
     CDS             complement(5077..6408)
                     /locus_tag="PKK95_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOC18541.1"
                     /translation="MQRIKTVIMGAAGRDFHNFNTIYRDNEQYEVIAFTATQIPNIEG
                     RKYPASLAGRLYPDGIPILAECELEGLIRERGVQEVVFAYSDVSYNYVMSRGALVNAA
                     GAHFKLLGARPTMIASTKPVISICAVRTGSGKSQTSRRVAHLLQRHGLKVAAVRHPMP
                     YGDLEKQKVQRFGSLDDLKKHDCTIEEMEEYEPHIVEGIVIYAGVDYGAILEQAQAEA
                     DVVIWDGGNNDMPFYRPDLAIVVADPLRVGNELSYYPAEANLRMADVVVINKIDSAEP
                     AAVKALRDNIRKVNRRAVIVDAASPLTVQHPERVTGKRALVVEDGPTLTHGEMKIGAG
                     TVAAEKLGASELVDPRPYTVGSITATFEKYPGIGVLLPAMGYGEQQVKDLEETIAKVP
                     CDVVVIGTPIDLNRVVRIKQPTVRVTYQLQEIGKPDLEDVVGRFVQEYVRK"
     gene            complement(6453..7643)
                     /gene="rocD"
                     /locus_tag="PKK95_09750"
     CDS             complement(6453..7643)
                     /gene="rocD"
                     /locus_tag="PKK95_09750"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOC18542.1"
                     /translation="MQTNDYIELENKYGAHNYHPLDVVISRAEGVWVYDVEGRRYLDC
                     LAAYSAVNQGHCHPKVLQALLDQAHKVTLTSRAFRNDQLALLYKDLHDLTGYDMALPM
                     NSGAEAVETAVKAARKWGYTAKGIPDGQAEIIVCANNFHGRTVTIVSFSTEAQYREGF
                     APFTPGFRVVPYGDAAAVAGAITPNTCAFLVEPIQGEAGIVVPPEGFLRETFEACRKN
                     RVLFVADEIQSGLGRTGKLFAFEHEGIWPDMVIVGKALSGGFYPVSAVLASKDVLGVF
                     KPGDHGSTFGGNPLACAVARAALGALVDEKMVERSAELGAYFLDRLRGLSSPAIREVR
                     GRGLWIGLEVEGPARPYCERLKEEGVLCKETHERVIRFAPPLVITRDEIDWAVERVER
                     VVCG"
     gene            complement(7688..>8001)
                     /locus_tag="PKK95_09755"
     CDS             complement(7688..>8001)
                     /locus_tag="PKK95_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18543.1"
                     /translation="LDAHDVWYALERDASKVLPEVDVVYQTRILPERMEDRAAHNRYT
                     IDARVLETMKRDALILHPLPRTVEIDKAVDADPRALYFRQANNGLYVRMALLTMVLDR
                     E"
BASE COUNT         1251 a         2722 c         2737 g         1281 t
ORIGIN      
        1 gctcgaggtc gtcctgcgcg agagccgcct gcgggaggac ctggttccga aggtcgtgga
       61 gctggcggca tcggtattcg acccgaggcg gctgcgcgcc gacaacccgt tcaagctcga
      121 acgcacgctc gaggggctcc ttcgcagctt cgagtacgaa atcgacaccg acagctttct
      181 ccggatcgtc ggcttgagcg acgagcagcc cgcggagctg cgcgccgaga tcgtcccgta
      241 caagaaggaa agggtcgtta ccaccgcgtc ggggctgatc aaccgcgacg cgacgtcgct
      301 gttcgccgcc atggagctgg cgggggaagg gccgcagctg tccatcgaac tggccgacgt
      361 cttcggcggc gagatcgact tcaactccga actgcagccg ggcgatacct tccgggtggc
      421 cttcgagaag gtgtatcgcg aggggacctt ttccgggtat ggcggcgtgc tggccgccga
      481 gttcgtgaac gtggggcact ccttcaaggc gctgcgctac acggtgcccg ggggcaagcc
      541 ggcgtactac gacgagcagg ggcgctcgct caagcgcttc ttcctcaagt cgccgctcaa
      601 gttcgaggct tcgatctcgt cgcggttcac gcattcgcgc cgccacccga tcctgcggat
      661 cgtgcggccg cacctcggcg tggactaccg tgcgccgatc ggcgccagcg tggtggcggt
      721 ggccaacggc accgtcgtct cggcggggtg gagcggcggc gcgggccgga tggtgacgct
      781 gcgccacccc gggggatggg agacgctcta catgcatctc tcgtcgatcg cggtgcgccg
      841 cgggcagcac gtgtcgcagg ggcagttgat cgggcgcgtg ggcagctcgg ggttgtccac
      901 cgggccgcac ctcgactacc gcgtccgcca gaacggccgc tacctgaacc ccctggtcgt
      961 gcaccgttcc ctgccgcccg gcgagccaat cccggccgcg cacctggcgg cgttccggga
     1021 ggagttcgaa cgctcttgga cgttgctctc ggcgacggag gccacgccgg tggaaggcgc
     1081 gccgggcgcg gtgaaggccg ccagcgagcc ggccgcagat ccgcccttgc ctaagaacgt
     1141 tcggtaatct accctatctg gcggtagatt gttgcctcag cctgccctcc tgtaactttt
     1201 acgcggctgc acgccgctgg cgagtcaaaa gcgatgtcca tgatccgtcc cttcacggcc
     1261 ttgaggccgc agccggacaa cgtcgatcgt gtgtcggcgg ttccctacga cgtggtgaac
     1321 accgaggaag cgcgcgcgat ggcgggtgac aacgccctca gcttcctgca cgtctcccgc
     1381 gccgagatcg acctgccgcc gtcgaccgac ccgtactccg acgccgtcta cgcgaaggcg
     1441 ctcgagaact tccagaggct gcgtgcggag gcgccgctgg tcgtggaaga ggcgccctcg
     1501 ttgtacttct accggctgag gatggggacc cacgagcaga cggggctggc cgcctgctac
     1561 tcggtggacg agtacgatgc cgggacgatc aagaagcacg agaagacgcg gcgcgacaag
     1621 gaggacgacc gcacccggca catcggcacg ctgcgggcgc agacgggtcc ggtcttcctg
     1681 acctaccgcg cgtcggccgc ggtggacgcg atcgcctcgc gggtgactgc cggggcgccg
     1741 ctgttcgact tcgagtcggc cgaccgggtg cgccacaccg tctggcgggc gtcgggggcc
     1801 gacgtggagg cgctggtgca ggcgttcgcc gagttgccct gcctgtacat cgccgacggg
     1861 caccaccggg cggccagcgc ggcgcggacg cggaagcacc tggccgggct ggagccgggc
     1921 gttgcccacc ccgaggccga ctggatgctg gcggtggcgt ttcccgacaa ccagacggcg
     1981 atcatgccgt acaaccgcgt cgtgaaggac ctcggcacct acacgcccga gacgctgctg
     2041 gcggggctgc gcgagaagct gcgcgtggtc gagggggcgg cgagcccggc ggcgaaggga
     2101 gaggtctcga tgtacctcgc cggccgttgg nnnnnnnnnn acacgatcgc gctgcagccg
     2161 cccgagggaa ccggcgacgt ggggagcacg ctcgacgtca gcctcctgca ggcgcaggtg
     2221 ctgacgccgt tcctggggat cggcgacccg cgaaccgaca agcggatcga cttcgtgggc
     2281 ggcatccggg gaacgggcga gctggagcgg ctggtgcggg aggggaaggc ggcggtggcc
     2341 ttctcgatgt tcccggtcgg ggtggacgac ttgatgcgga ttgcggacgc gggcgaaatc
     2401 atgcccccga agtcgacctg gttcgagccg aagctgcgtg acggactcct ttcccatctc
     2461 atctagccgg gcccgcgagg cgcggcccaa caaggacggg cagacatgaa agtcctggtg
     2521 gcggacaagt tcgagaagag tggcatcgag ggtctgaagg cggcgggctg cgaggtcatc
     2581 ttcgaacccg acctcaagga cgccgcgctg gccgaggcga tcggccggac gggcgccgac
     2641 gtcctggtgg tgcgctcgac ggtggtgacc gagccgatga tgaacgcggg gcggctggcg
     2701 ttgatcgtgc gggcgggagc cggctacaac acgatcgacg tgaaggccgc gagccgccgc
     2761 ggcatctacg tgtcgaactg ccccggcaag aactcggtgg cggtggccga gctggcgttc
     2821 ggcctgatga tcgcgctcga ccggcggatc cccgacaacg tcgccgagct gcagcgcggc
     2881 aagtggaaca agaaggagta ctcgaaggcg agggggctgg cgggctcgac gctcggcctg
     2941 ctcggtttcg gcaagatcgg ccaggagatg gccaagcggg ccatggcgtt ccacatgaac
     3001 ctgatcatct ggagcgagct gggcgtccgc caggaccgcg acgcgatccc ggtggacctc
     3061 ccgctgctgc tcgagatgcg gcgcggccac gacgcctcgg ggctcatcca ggagcggatc
     3121 tcggtggtca acagcccggc cgaggttgcc gagaagtgcg acgtgctgag catccacctg
     3181 gcctcgagcc cgctcacgaa gaacatcgtg aacgccgagg tgatcggcaa gctgaagccg
     3241 ggcgcgtacg tcatcaacac tgcccgcgcc gacgtcgtcg actacgacgc gctggccagg
     3301 gcggtggtgg agaagggggt ccgggtcggc ctcgacgtgt accccggcga acccgcgtcg
     3361 cccaccgccg actacgcgca cccgctgctc ggtctgcccg gcgtgtacgg gacgcaccac
     3421 atcggcgcct ccaccaacca ggcgcaggag gcgatcgccg aggagaccgt tcggatcgtc
     3481 accgcctaca aggagaccgg gcgggtgccg aacgtggtca acctcgccaa gaagacgccg
     3541 gccacccact cgctgatcgt gcggcaccgc gaccagccgg gggtgctggc ctgcctgttc
     3601 gccaagctcg ccggggcggg gatcaacgcg cacgaggccg agaacatcgt cttcgagacc
     3661 gccgaggcgg caatcgcgcg gatcaacgtc gaccgcgagc cctcggccgc cctgatcgag
     3721 tcgttccgca acgactgccc ggcggtgtac gaggtgaaga tcgtcgcgct ggcgtagcgg
     3781 agcctcgacc ttgtggcttc gacctcgtga cctcgcgact tcgacctcgt gacttcgcga
     3841 cttcgtgact ttggtgactt cgtgacttcg tgacttcgtg acgctggaga ctgccatggt
     3901 taccaccact cgcgtgttca acttcgcggc cggccccgcc gtcctgccgc tgcccgccct
     3961 cgaggaggcg cagcgcgacc tggtcgcgct ccccaatgtc gggatgtcgg tgctcgagat
     4021 cagccaccgc tcgaagacct tcgaggccat catccaggag tgcgaggcga acgtcaggaa
     4081 gctcgccaac gtccccgaga actaccacgt gctgttcctg caggggggcg ccaccacgca
     4141 gttctcgatg gtgccgatga acctccttcc ccccggcgcc tgggccgact acatcgtcac
     4201 cggctcgtgg tcgtcgaagg cggtgaagga ggcgaagaag gtcggcgccg tcaacgtcgc
     4261 cttcgacggc aagggcgaga acttcgtccg cgtcccgaag gccggcgagc tgaagctctc
     4321 gaaggacccg gcctacgtgc acgtcaccac caacgagacg atccacgggg tcgagttcca
     4381 cgagctgcca gacgtcggcg agcgcgtgct ggtggccgac acctcgtcgc acatgttcag
     4441 ccggccgatc gacgtgacga agtacggcct catctacgcc ggcgtgcaga agaacctcgg
     4501 cccggccggc gcgaccatgg tgatcgtccg cgacgacctg gtgaagaagg cggccgagta
     4561 caaggggtac gcgaacctgc ccgtcatgct gcagtacggg acccacgtcc ccgagaagtc
     4621 gctctacaac accccgccgg tgttcaccat ctacatggtg ggcctggtga tgaagtggct
     4681 ggtggggctc ggcggcctcg aggccatgga gcggatcaac atccgcaagg ccgagaagct
     4741 gtacgccgag atcgaccgct ccggcttcta ccgcggccac gcggccaagg acagccggtc
     4801 gcggatgaac gtcaccttcc ggctgccgag cgaggagctg gaggcgaagt tcgtcaagga
     4861 gtcgaaggcg gcggggctcg acgggctgaa gggccaccgc tcggtgggcg gcctgcgcgc
     4921 ctcgatctac aacgccttcc ccgaggaagg ggtggacgcg ctggtggcgt tcatgcagga
     4981 gttcgagaag aagaacggct gagggcagct gacagctgac ggcttgcgcc ggaaagcgca
     5041 gggggcgacc ctggcggtcg ccccctgcgc cgcctgctac tttctcacgt actcctgcac
     5101 gaacctcccc accacgtcct cgagatcggg cttgccgatc tcctgcagct ggtaggtgac
     5161 ccgcacggtc ggctgcttga ttctcaccac gcggttcagg tcgatcgggg tcccgatcac
     5221 caccacgtcg cacggcacct tcgcgatcgt ctcctcgaga tccttcacct gctgctctcc
     5281 gtaccccatc gcgggcagca ggacgccgat gcccgggtac ttctcgaagg tcgctgtgat
     5341 cgagccgacg gtgtaaggcc gcggatcgac cagctcggag gcgccgagct tctcggccgc
     5401 taccgtgccc gccccgatct tcatctcgcc gtgggtgagc gtcgggccgt cctcgacgac
     5461 cagcgcgcgc ttgccggtca cccgctccgg gtgctgcacg gtgagcggtg acgcggcgtc
     5521 gacgatgacc gcccggcggt tcaccttgcg gatgttgtcg cggagcgcct tcacggccgc
     5581 cggctcggcg ctgtcgatct tgttgatgac gacgacgtcg gccatgcgca ggttggcctc
     5641 ggcggggtag tagctcagct cgttgcccac cctcagcggg tcggccacga cgatggccag
     5701 gtcgggccgg tagaacggca tgtcgttgtt tccgccgtcc cagatgacca cgtcggcctc
     5761 ggcctgcgcc tgctcgagga tggcgccgta gtccactccg gcgtagatga cgatcccctc
     5821 cacgatgtgg ggctcgtact cctccatctc ctcgatggtg cagtcgtgct tcttcaggtc
     5881 gtccaggctg ccgaaccgct ggaccttctg cttctcgagg tcgccgtagg gcatcgggtg
     5941 ccgcacggcc gccaccttca aaccgtgccg ctgcagcagg tgcgccaccc ggcggctggt
     6001 ctggctcttg ccggagccgg tgcggacggc gcagatcgag atcaccggct tggtgctcgc
     6061 gatcatcgtc gggcgggccc cgagcagctt gaagtgcgcg ccggcggcgt tcaccagcgc
     6121 gccgcggctc atcacgtagt tgtaggagac gtcgctgtag gcgaagacca cctcctgcac
     6181 gccccgctcg cggatcagtc cctcgagctc gcactcggcg aggatcggga tcccgtcggg
     6241 atagagccgg cccgcgagcg acgcggggta cttccgcccc tcgatattcg ggatctgggt
     6301 ggccgtgaag gcgatgacct cgtactgctc gttgtcgcgg tagatggtgt tgaagttgtg
     6361 gaagtcgcgc cccgcggccc ccatgatcac ggtcttgatg cgctgcatga atgaatgcct
     6421 ttcaatcagg atcccgggtc ctgaatcctt catcacccac aaaccacccg ttccacccgc
     6481 tcgaccgccc agtcgatctc gtcgcgcgtg atgaccagcg gcggcgcgaa ccggatcacc
     6541 cgctcgtggg tctccttgca gagcacgcct tcctctttca gccgctcgca gtagggccgc
     6601 gccggcccct ccacctcgag gccgatccag aggccgcgcc cgcggacctc acgaatcgcc
     6661 gggctcgaga ggccccgcag ccggtccagg aagtacgcgc ccagctcggc cgagcgctcc
     6721 accatcttct cgtccacgag cgcgccgagc gccgccctcg ccacggcgca ggcgagcggg
     6781 ttgccgccga acgtgctgcc gtgatcgccg ggcttgaaga cgcccagcac gtccttcgag
     6841 gcgagcaccg ccgacaccgg gtagaagccg cccgagagcg ccttcccgac gatcaccatg
     6901 tcgggccaga tcccctcgtg ctcgaaggcg aacagcttcc cggtgcgccc gaggcccgac
     6961 tggatctcgt cggcgacaaa cagcacccgg ttcttccggc acgcctcgaa ggtctcgcgc
     7021 aggaacccct cgggcggcac cacgatcccc gcctcgccct ggatcggctc gaccaggaac
     7081 gcgcaggtgt tcggcgtgat cgcccccgcc acggcggcgg cgtcgccgta cggcaccacc
     7141 cggaagcccg gcgtgaaggg ggcgaacccc tcgcggtact gcgcctcggt cgagaagctc
     7201 acgatggtga cggtccgccc gtggaagttg ttggcgcaga cgatgatctc cgcctgcccg
     7261 tcggggatcc ccttggccgt gtacccccac ttcctggccg ccttgaccgc cgtctccacc
     7321 gcctcggccc ccgagttcat cgggagcgcc atgtcgtacc ccgtcaggtc gtgcaggtcc
     7381 ttgtagagca gggcgagctg gtcgttgcgg aacgcccgcg acgtgagcgt gaccttgtgg
     7441 gcctggtcga gcagcgcctg caggaccttc gggtggcagt gcccctggtt gacggccgag
     7501 taggcggcca ggcagtcgag gtaccggcgc ccctcgacgt cgtacaccca caccccctcc
     7561 gcccgggaga tcaccacgtc gagcgggtga tagttgtgcg ctccgtactt gttctcgagc
     7621 tcgatgtaat cgttggtctg catgtcgtgt ctctccagcc gctgcctcag ggctccccga
     7681 ctccgtccta ctccctgtcc agcaccatcg tcagcagcgc catccggacg tagaggccgt
     7741 tgttcgcctg gcggaagtag agggcgcgcg gatcggcgtc caccgccttg tcgatctcca
     7801 ccgtgcgggg cagcgggtgc aggatcaagg catcccgctt catcgtctcc agcacccgcg
     7861 catcgatcgt gtagcggttg tgcgcggccc ggtcctccat ccgctccggc aggatgcgcg
     7921 tctggtacac gacatccacc tcgggcagca ctttcgaggc gtcgcgctcg agcgcgtacc
     7981 agacgtcgtg cgcgtcgagg t
//
LOCUS       DAOPQX010000162         8000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10093_length_8000_cov_4.429075, whole genome shotgun sequence.
ACCESSION   DAOPQX010000162 DAOPQX010000000
VERSION     DAOPQX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10093_length_8000_cov_4.429075"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PKK95_09760"
     CDS             complement(<1..894)
                     /locus_tag="PKK95_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOC18544.1"
                     /translation="MTFLDLETHPGGGRVQLIATLGPRSLAIAAELASGGATAFRLNA
                     SHLTSAALEESLAALRHACPTAPIVVDLQGAKMRLGWIRPLAVTEGDRLRFSLDAGEG
                     TSLPHPEIYAQVTPGDVLSVDDGRVRLRVVRAGETWLEARALGGGTLAPRKGVNVEQH
                     PVRLAGLTPADRDSIDVASRGGVRAFAFSFMETGEEAEWVRSQAPGALVVGKIEREES
                     LRALPAIARRTDAVWVCRGDLGAQLGPARLAREVAAISPGACGAPLLMAGQVLQHLVD
                     PDEPTRSGVCHLPDLPARGYAG"
     gene            complement(1032..1853)
                     /locus_tag="PKK95_09765"
     CDS             complement(1032..1853)
                     /locus_tag="PKK95_09765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOC18545.1"
                     /translation="MNVSFLRRSLVIVIALPVLGLCSPLGAWSQTPAADTIDVVNPPA
                     DAVKSSTGLASKVLIAGSGTKKPGPSDLVNIVYVVRTKDGRTLSSGPAAKMQLSSIQV
                     PGLAEGLQEMVAGEKRRLWMSQDLAFKGQSGRPAGPVVFDVTLIEVIPPPEVPADVAA
                     PPADAEKTKSGLASKVIKPGSGDDHPSKKSKVTVHYSGWTTDGQMFDSSIVRGKPASF
                     PLDQVIAGWTEGVQLMVPGETRRFWIPEKLAYQGQDGKPKGMLVFDVELISIDGK"
     gene            complement(1970..4348)
                     /locus_tag="PKK95_09770"
     CDS             complement(1970..4348)
                     /locus_tag="PKK95_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18546.1"
                     /translation="MRTRRWVSVLVPLAFLLSAGPGRLGARAPQAPGATDGAFRLKAF
                     QQHQALAEASPFKTLEWQFLGPRNISGRCTDVAVLTPRGRSYAIFVGTASGGLWKSVN
                     EATTWQPVLENAASTAIGDVAVAPSNPSVVWVGTGEANIYRSSQSGIGVYKSVDGGRS
                     FTHKGLADTGTIARIVIHPKNPDVVWVAASGREWTTNPGRGVFQTADGGNTWRHVLKV
                     DEQTGAIDLVMDPSDPDTLYASTWQRTRLKWNDPRTLPGYRGSGIHKTTDGGRTWRAI
                     DKGLPAPDKRGRIGLDVCLTSPNVLYALVDNYEIAREPTDEEKNDPYGLPSSGFVKGA
                     TVYRSADRGESWTQVSGLTPEQKALMERHSGTFGWVFGQVRADPSDANTVYTLGVRLH
                     VSSDGGRTFKTLDTPGVDHHGMWIDPENSDYLVIAYDQGFAISYDRGLSWKDSRLTLP
                     VAQFFNVSYDMDTPFRVYGSMQDHGSFRAAVDLSAGRDRIPVAEFEEAPGGEGSTHAI
                     DPEDPQTVYSSGFYGTLTRARVGRSGTARTIKGLLPSRYPEEGPLRGQWLAPTILSPS
                     NPDIVYHGMQQLLMSRDRGNTWEAISPDLTYGPEAAQQKGDIRFHTIFTISESPLRFG
                     LIYVGTDDGRVHVTKDGGKAWTEIVNGIAPRRWISRLAASAFDLGTVYMTQNGKRDDD
                     FTAYVWKSTDYGRTWTSIAAGIPMGPVNVIREDPVNRQVLYVGTDTGVYVSADGGSSW
                     TVLGAGLPSAYVHDLVIHPRDNVLVAATHGRGMWAIDVNAVNEKEERRRRRE"
     gene            4549..6174
                     /locus_tag="PKK95_09775"
     CDS             4549..6174
                     /locus_tag="PKK95_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC18547.1"
                     /translation="MYQPIPERTLAALLDYSGRLAAALTVGGVAAVVVEEGRGAVGAC
                     RAEVALFDPLGCALRVVASSGGAMEARAETSQDAMAEAARTRMPIYGAGSPVSASLPL
                     VAGEQVIGAARFVFADDGDLSERDRALVALVARQAADALQRARLYAAEREAREEAESA
                     NQMKDEFLATLSHELRTPLNAIMGWAYMLRHGSLDEATSARALDAILRNAHAQNQLVA
                     DVLDVSRIITGRLRLHVRPVDLRGVVQQALDAVAPAVAARGVNVELVTGSGDMVIAGD
                     PDRLQQVVWNLLSNAVKFTPAGGRVEVKLDRAECGVDLVVIDTGAGIAPEFLPRVFDR
                     FSQADASTRRTHGGLGLGLSIVRHLVELHGGSVEAWSEGVGLGARFTVRLPASAAADQ
                     ARQPARRPQSRGAAGTGHVNLQGLRVLVVDDEPDGREVVVSILRQRGAHVVAAASAAE
                     GLERLAETLPHLLIADIGMPGMDGFELIRRVRALPLPAGNVPAMALTAYGRAEDRARC
                     LLMGFQVHVPKPFVPDDLVAAVDRLLSGVTSSQ"
     gene            complement(6345..7844)
                     /locus_tag="PKK95_09780"
     CDS             complement(6345..7844)
                     /locus_tag="PKK95_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOC18548.1"
                     /translation="MKRVALALLIAPVILIAGPGGWEPWPSGTPALQARAIEVRDSVA
                     IATAFRVPGNYQILPGTYRGNFVVSADDVTVEATGVRFEPEDRGRAALTILGSRVRIT
                     GLVVANGAPDRDTINVGANGIATLEELPRDVTLVRVTAEAGPNGGHRGFGLHGIDIKL
                     LDCRATGFWENGRDSQAIWINNGPGPYLVEGGYFEASGENIMVGGALFGIRNPAVIPS
                     DITIRHAHLKKPLEWQGGEGRPKRGTVKNLFELKAGQRVLVEDCLLENNWKDGQAGSA
                     ILIKADNQEGVTPWVVTRDVTFRRNVIRNSPDSYVVNIRGIHPASPTRRTANVTFEHN
                     LFVGVTNGIQAGDGVGGLRVVRNTFLTITGTFLNFYGTGVDQGGEDEERARAVERPPA
                     AHDPQRAPIDPPHRLPDRPRPSGRFRTPLTFVGNVLHSGAYGVKGEGKASGIDTLEYY
                     AQVLDFSRNVIERGSRAIPYPPGNTIVGPGALLGLLDPSSLKYPGDAGY"
BASE COUNT         1134 a         2741 c         2830 g         1295 t
ORIGIN      
        1 tcccgcgtag ccgcgcgcgg gcaggtcggg gaggtggcac acccccgagc gcgtcggctc
       61 gtcgggatcc accaggtgct gcagcacctg gcccgccatc aggaggggag ccccgcaggc
      121 gccaggcgag atcgcggcga cctcgcgcgc gagccgggca ggacccagct gggcccccaa
      181 atcgccccgg cacacccaca cggcgtcggt gcgtcgcgcg atcgccggca gcgcccgaag
      241 cgactcctcg cgctcgatct tcccaaccac cagcgcgccg ggcgcctgcg agcgcaccca
      301 ctccgcttcc tccccggttt ccatgaacga gaaggcgaag gctcgcaccc ccccgcggga
      361 ggcgacgtcg atcgagtcgc ggtcggctgg ggtgaggccg gcgagacgca cggggtgctg
      421 ctcgacgttg acgcccttgc gcggcgccag cgtgccgccg ccaagtgccc gcgcctcgag
      481 ccacgtctcg cccgcgcgga cgacccgcag ccgcaccctc ccgtcgtcca ccgacaggac
      541 gtcgccgggc gtcacctgcg cgtaaatctc cgggtgcggg agggaggtgc cctcgccggc
      601 gtcgagtgag aagcgcagac ggtcgccctc ggtgaccgcg agcggccgga tccacccaag
      661 gcgcatcttc gctccctgca ggtcgacgac gattggggcc gtcgggcagg cgtgccgcag
      721 ggcggccagg gactcttcga gggccgcgga cgtgaggtgc gaggcgttca ggcggaaggc
      781 cgtcgctccc ccggacgcca gctccgccgc tatcgccagc gaccgcggcc caagcgtggc
      841 gatcagctgc acgcggccgc cgccggggtg cgtctcgaga tcgaggaacg tcatgtggtc
      901 acgacgagga tcgggagccg cggaggaagg gggcggaccg gcgcgtgctc cacgtgcgcc
      961 ggtgcgcccc ggtccgggaa ggggagggcc ggccgccctg cgggaagacc ggcccgatgc
     1021 ggccgagcgg gctacttgcc gtcgatcgag atcagctcga cgtcgaacac gagcattccc
     1081 ttcggcttgc cgtcctgtcc ctggtaggcc agcttctcgg ggatccagaa ccggcgcgtc
     1141 tcgcccggga ccatgagctg cacgccctcg gtccacccgg caatgacctg gtcgagcggg
     1201 aagctggcgg gcttgccgcg cacgatcgag ctgtcgaaca tctgcccgtc ggtcgtccac
     1261 ccgctgtagt gcacggtgac cttgctcttc ttcgacgggt ggtcgtctcc cgagcccggc
     1321 ttgatcacct tcgacgccag gccggacttg gtcttctcgg cgtccgccgg cggggcggcc
     1381 acgtcggcag gcacctccgg cggcgggatg acctcgatca aggtgacgtc gaacaccacc
     1441 ggcccggccg gacggcccga ctggcccttg aacgccaggt cctgcgacat ccagagacgg
     1501 cgcttctcgc cggcgaccat ctcctggaga ccttcggcca gcccgggcac ctggatgctg
     1561 ctgagctgca tcttggcggc gggccccgac gacagcgtgc ggccatcctt ggtgcgcacc
     1621 acgtacacga tattgaccag gtcgctcggc cccggcttct tggtgccgct gccggcgatc
     1681 aagaccttcg aggcgaggcc agtggagctc tttaccgcgt cggcaggcgg gttgacgacg
     1741 tcgatggtgt cggcggccgg ggtctgcgac catgcgccaa gaggactgca cagcccgaga
     1801 accgggagag cgatgacgat gactagtgaa cgcctgagga atgacacatt cataaccagc
     1861 gatcgtgtca gtttcgccgg ggtggcgcaa cactttactg cggcaggggt ggcacccgac
     1921 ccgccccgcg cctgccacgc gcctgcgctt cgcgcggtgc cccgcaccgt cattcccgcc
     1981 gcctgcggcg ctcttctttc tcgttcaccg cgttcacgtc gatcgcccac atcccgcgcc
     2041 cgtgcgtggc ggctaccaac acgttgtcgc gcgggtggat cacgaggtca tggacgtagg
     2101 cggacggcag gccggccccg agcaccgtcc agctcgagcc gccatccgcg ctgacgtaca
     2161 cccccgtgtc ggtgcccaca tagagcacct gtcgattcac cgggtcctcg cggatgacgt
     2221 tcaccggtcc catcgggatc ccggcggcga tgctggtcca ggtgcggccg tagtcggtgg
     2281 acttccagac gtaggccgtg aagtcgtcgt cgcgcttgcc gttctgcgtc atgtagacgg
     2341 tgcccaggtc gaaggccgac gcggccagcc gcgagatcca gcggcgcggg gcgatgccgt
     2401 tcacgatctc ggtccacgct ttcccgccgt ccttcgtcac gtggaccctg ccgtcgtcgg
     2461 tcccgacgta gatcaggccg aagcggagcg gcgattcgga gatcgtgaag atggtgtgga
     2521 agcggatgtc gcccttctgc tgcgcggcct ccggcccgta ggtcagatcg ggactgatcg
     2581 cctcccaggt gttgccccgg tcgcgcgaca tcagcagctg ctgcatgccg tggtacacga
     2641 tatcggggtt gctgggggag aggatggtcg gcgcgagcca ctggccgcgc agcgggccct
     2701 cctccgggta gcgcgacggg agcagcccct tgatcgtgcg ggctgttccg gagcggccga
     2761 cgcgggcgcg ggtgagcgtg ccgtagaagc cagaggagta gacggtctgc gggtcctcgg
     2821 gatcgatggc gtgcgtcgag ccctcgcccc ctggcgcctc ctcgaactcg gcgacaggga
     2881 tgcggtcgcg gcccgcggac agatccaccg ccgcgcggaa gctgccgtga tcctgcatcg
     2941 atccgtagac gcggaacggc gtgtccatgt cgtacgacac gttgaagaac tgcgcaaccg
     3001 ggagcgtgag gcgcgagtcc ttccagctga gcccccggtc gtaggagatc gcgaagccct
     3061 ggtcgtaggc gatgacgagg tagtcgctgt tctccggatc gatccacatc ccgtggtgat
     3121 ccacgcccgg cgtatcgagc gtcttgaacg tccggccgcc gtccgacgag acgtggaggc
     3181 gcacaccgag cgtgtagaca gtgttggcgt cgctcgggtc ggcgcgcacc tgtccgaaga
     3241 cccagccgaa cgtccccgag tgacgctcca tcagcgcctt ctgctcgggc gtcaggccgc
     3301 tcacctgcgt ccagctctcg ccccggtcgg cggagcggta cacggtcgcg cccttgacga
     3361 agccgctcga cggcagcccg tacggatcgt tcttctcctc gtcggtcggc tcgcgagcga
     3421 tctcgtagtt gtccacgagc gcgtagagca cgttcgggct cgtgaggcag acgtcgaggc
     3481 cgatgcggcc ccgcttgtcg ggcgccggca gccccttgtc gatcgcccgc caggtgcgcc
     3541 cgccgtcggt cgtcttgtgg atgccgctgc cgcggtagcc gggaagggtg cgcggatcgt
     3601 tccacttgag gcgcgtgcgc tgccaggtgg aggcgtagag ggtgtccgga tcggacgggt
     3661 ccatcaccag gtcgatggcc ccggtctgtt cgtccacctt caggacgtgg cgccacgtgt
     3721 ttccgccgtc ggccgtctgg aacacgcctc gcccggggtt ggtcgtccac tcgcgccccg
     3781 aggccgccac ccatacgacg tccgggttct tcggatggat gacgatgcgg gcgatggtgc
     3841 ctgtgtcggc aagccccttg tgtgtgaagg accgccctcc atcaacggac ttgtagactc
     3901 caatgcccga ctgcgagctg cggtagatgt tcgcctcgcc ggttccgacc cagaccacgc
     3961 tgggattcga cggcgccacc gcgacatcac caatcgccgt cgatgcggcg ttctcgagaa
     4021 cgggctgcca cgtggtggcc tcgttcacgc tcttccacag gccgcccgag gccgtgccga
     4081 cgaagatcgc gtaactgcgg ccgcgcgggg tcaacaccgc gacgtcggtg cagcggccgc
     4141 tgatgtttct cgggccgagg aattgccact cgagtgtctt gaacggcgac gcctcggcca
     4201 gcgcctggtg ctgctggaac gctttcagcc ggaaggcgcc gtcggtggcg ccgggcgcct
     4261 ggggcgcgcg ggcgccaagc cggcccggac cggctgagag caggaaggcc aagggaacga
     4321 ggaccgacac ccagcgtcgc gtgcgcatgc cacctccgcc tcgggggttc gcgggcggcc
     4381 cttgaagccg ccccgacttt gcgcggcgga ttgtagcacc cgaaggtgcg gatcttgctc
     4441 ttttgccacg aaggccccct caacgacgtt ctggtgtcgg cgtgacggtc agtgcccgtg
     4501 cacgctgtcg ccttttccta caggggggcg tcccccccag cctgccgaat gtaccaaccg
     4561 atcccggaac ggacgcttgc ggccctgctc gactacagcg ggcgcctggc cgccgcgctc
     4621 accgtgggcg gcgtggcggc cgtcgtcgtc gaggaaggcc gtggcgcggt tggagcgtgc
     4681 cgcgccgaag tggcgctctt cgatccgctg ggctgcgctc tacgcgtcgt ggcgtcgagc
     4741 gggggggcga tggaagcccg ggcggagact tcgcaggatg cgatggccga ggccgcccgc
     4801 acgagaatgc ccatctatgg ggccggcagc ccggtgtcgg catcgctccc gctggtggcg
     4861 ggggagcagg tcatcggcgc ggcgcgcttc gtgttcgcgg atgacggcga cctctccgag
     4921 cgcgatcggg ccctggtggc gctcgtcgcc cgccaggccg ccgatgcgct gcagcgggcg
     4981 cggctgtacg cggccgagcg ggaggcgcgg gaggaggcgg agtcggccaa ccagatgaag
     5041 gacgagtttc ttgcgacgct gtcccatgag ctgcgcacgc cgctcaacgc gatcatgggg
     5101 tgggcgtaca tgctgcggca cgggtcgctc gacgaagcga cctccgcgcg ggcgctcgac
     5161 gcaatccttc gcaacgcgca cgctcagaac cagctggtgg ccgatgttct ggatgtctcg
     5221 cgcatcatca cgggccgcct gcgcctccac gtccggccgg tggatctgcg cggcgtcgtt
     5281 cagcaggcgc tcgacgcggt ggcccccgct gtcgcggctc gcggcgtcaa cgtggagttg
     5341 gtcaccgggt ccggcgatat ggtgatcgcc ggggaccccg accggctgca gcaggtggtt
     5401 tggaacctgt tgtcgaacgc cgtgaagttc acccccgccg gcggccgtgt cgaggtcaag
     5461 ctcgatcgcg ccgagtgcgg cgtcgacctg gtcgtgatcg acacgggcgc cggcatcgcg
     5521 cccgagttcc tgccgagggt gttcgatcgg ttctcgcagg ccgatgcctc gacgcggcgc
     5581 acgcacggcg ggctgggcct gggactctcg atcgtgcgac acctggtgga actgcacggt
     5641 ggcagtgtcg aggcgtggag cgaaggggtc gggctgggcg cgcgcttcac ggtgcggttg
     5701 cccgccagcg cggccgcgga ccaggcaagg cagcccgcgc gtcggccgca gtcgcgaggc
     5761 gccgcgggca cgggccacgt caacttgcag ggcctgcggg tcctggtggt cgacgacgag
     5821 cccgacgggc gagaggtggt ggtctcgatc cttcgtcagc gcggcgcgca cgtggtggcg
     5881 gcggcctcgg cggccgaggg cctcgagcgc ctcgccgaga cgttgccaca cctgttgatc
     5941 gcggacatcg ggatgcctgg catggacggg ttcgagctca tccgccgggt gcgcgcgctg
     6001 ccgctgccgg ccgggaatgt tcccgcgatg gcgctcactg catacgggag ggcggaggat
     6061 cgcgcgcggt gcctgctcat gggcttccaa gtgcacgtgc ccaagccttt cgtccccgac
     6121 gacctggtcg ccgcggtcga tcgtcttctc tccggcgtga cctcctcaca gtagcgccgg
     6181 cgtctcgccc gggaagggcc gccgcgttga acgtggacag gccgccgcga cagccacgta
     6241 cgtttcaccg caaagacgca gaagacgcag agtcttgaat gatagagatc tccgcgtacg
     6301 cctccgcgcc tccgcgtctg cgttgacgtg gacgagccgg cacgtcagta ccccgcgtcg
     6361 cccgggtatt tcaaactgct cgggtcgagc aacccgagca gcgcgcccgg cccgacgatc
     6421 gtgttgcccg gcggataggg gatggctctc gaaccgcgct cgatgacgtt gcgggagaag
     6481 tccaggacct gcgcgtagta ctcgagcgtg tcgatgccgg acgccttgcc ctctcccttg
     6541 acgccgtacg cgcccgagtg caggacgttc ccgacgaagg tcagcggcgt tcgaaagcgt
     6601 ccgctcgggc gcggccggtc tggcaaccgg tgtgggggat cgattggggc acgctgcgga
     6661 tcgtgggcgg ccggcggccg ctcgaccgcc cgcgcacgct cctcgtcttc gccgccctgg
     6721 tcgacacccg tcccgtagaa gttgaggaag gtgccggtaa tcgtcaggaa cgtattccgg
     6781 accacgcgca ggccgcccac cccgtcgccc gcctggatcc cgttggtgac gccgacgaag
     6841 aggttgtgct cgaaagtgac gttcgcggtg cgccgtgtcg gagacgccgg gtggatgcct
     6901 cggatgttca ccacgtagga gtcgggggag ttcctgatca cgttccgccg gaacgtgacg
     6961 tcgcgcgtga cgacccacgg ggtgacgcct tcctggttgt cggccttgat caggatggcg
     7021 cttcccgcct ggccgtcctt ccagttgttc tcgagcaggc agtcctcgac caacacccgc
     7081 tgaccggcct tcagctcgaa gaggttcttc acggtgccgc gcttcggcct tccctcgccg
     7141 ccctgccact cgagcggctt cttcagatgg gcatggcgaa tcgtgatgtc gctggggatg
     7201 acggcggggt tgcggatgcc gaagagcgcg ccgcccacca tgatgttctc gccggaggcc
     7261 tcgaagtatc cgccctcgac caggtacggg ccgggaccgt tgttgatcca gatcgcctgg
     7321 ctgtcgcggc cgttctccca gaatccggtg gcgcggcagt cgagcagctt gatgtcgatg
     7381 ccatgcaggc cgaagccgcg gtgaccgccg ttgggcccag cttcggcggt gacccgcacc
     7441 agcgtgacgt cgcgcggcag ctcctcgagc gtggcgatgc cattggcgcc gacgttgatc
     7501 gtgtcgcggt ccggggcgcc gttcgcgacc accaggccgg tgatgcgaac ccggcttccg
     7561 aggatggtga gggcggcccg tcctcgatcc tccggttcga accgcactcc ggtagcctcc
     7621 acggtcacgt cgtctgccga gacgacgaag ttgccgcggt aggtgccggg aaggatctgg
     7681 tagttacccg gcacccggaa agccgtcgcg atcgccacgc tgtcgcgcac ctcgatcgca
     7741 cgcgcctgca gtgccggcgt ccccgacggc cagggttccc agccgccggg gccggcgatg
     7801 agaattaccg gcgcgatcag cagcgcgaga gcaacacgct tcatggttgg gctgacacct
     7861 cgtcgatcga acatccgctg caatctcgct gccggcactt ccgcacgtcc ggcggccagc
     7921 actcacgaac gcttgtcttg gaaggtttag gtggtggcca ggcccagagt cgaactgggg
     7981 acaccgtgat tttcagtcac
//
LOCUS       DAOPQX010000163         7987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10112_length_7987_cov_4.402547, whole genome shotgun sequence.
ACCESSION   DAOPQX010000163 DAOPQX010000000
VERSION     DAOPQX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10112_length_7987_cov_4.402547"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1328)
                     /locus_tag="PKK95_09785"
     CDS             complement(165..1328)
                     /locus_tag="PKK95_09785"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOC18549.1"
                     /translation="MTVRLGSERLLASHLLDNRRVGIVANPASIDGSFRHVVDRLASH
                     PSAKLAGIFGPQHGFRADVQDNMIESPHGHDPARRVPVYSLYSETREPTAEMLDGLDL
                     LVIDLQDVGARIYTFIYTMANCMKAAARHGVRVVVCDRPNPIGGEAVEGPVLERGFES
                     FVGLFPIPMRHGMTVGELARLFNEEYGLGADLEVVPMEGWQRGMYWDDTGLPWVIPSP
                     NMPTLDTAIVYPGAVLFEGTQLSEGRGTTRPFEILGAPWIDAEPFAAGLNAQAIEGAH
                     FRPVVFEPTFHKHAHQACGGVQIHVTDRRTFRPVLASVAVLGAFHDANPERFAWRQPP
                     YEYEHRLMPIDILAGSEALRKGIEAGRSFRDIAAEWEGPTAEFEQTRQKYLLY"
     gene            complement(1390..3678)
                     /locus_tag="PKK95_09790"
     CDS             complement(1390..3678)
                     /locus_tag="PKK95_09790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOC18550.1"
                     /translation="MPSAGPVRSMCLVAALLVVGSLAVSPPARGQGTPAKRPMTFLDV
                     QNLEQVGAPRSSPDGRWLLYTLNKPDWKEARRQSDIYVVSIEKGIDSTRQLTFSGDKN
                     EAQPTWARDGSFFVYASNRDAPTSKGEQNQLYLMRIDGGEGRRITDAKEGVSTFAFSR
                     DGAWLVYRSGKAGEEQLYRLSVKGIEDAKAEQLTRHEGAVGAWRWSPDGRRIYFVAPE
                     RRDADEKQRREKKFTVEVRNAETPIESLWALDLESKKVERLTADASYSVAGFTVSDDG
                     RWIAFSGVAAERYKRNVTEQGIYADPYLLEVATGQIERLVANEEVGEGLPSFSPDGRY
                     MAFSAPDDLTQYSMKNRRVYLRPVGDRGGKWRKLGSSFDGDVGIGFWSKDGGTIYFNQ
                     GIKATTQLFALDVVKDTVRQITYEKAALAVDRDEDSGVLLIDYSDQATPRSVYTVASV
                     EQAGTRAAWRPLIESNPQVRDLALGEQEEITWKSTDGRMVGGVLVKPVGYEKGKRYPL
                     IVAIHGGPAGADVLRFNGGYGSQVYAGAGYAVLMPNYRGSTNYGEAHKTAIVGNYFPP
                     GFDDIMTGVDHLIAQGIVDGSRMGALGWSAGGHWSNWILTHTDRFKAISSGAGTSNWI
                     SMYAQSDVQRNRQYYLGNRLPYDDFEPYWAQSPIRYVRNAKTPTMIHVVEGDPRVPSP
                     QSVELHMALKRLGVPTELFVYPGKSHGIPDQRNQLVKAVSEMAWMDYYVRGVGEKFSW
                     RMVLDTLPGEEAGKKREAEGEK"
     gene            3900..4118
                     /gene="infA"
                     /locus_tag="PKK95_09795"
     CDS             3900..4118
                     /gene="infA"
                     /locus_tag="PKK95_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOC18551.1"
                     /translation="MSKEDLLDIPGTVTSIHGGGLYRVQADAGQEILAQLSGRMRRFR
                     IKVVTGDRVTVSVSPYDMVRGIITFRFR"
     gene            4398..6239
                     /gene="ftsH"
                     /locus_tag="PKK95_09800"
     CDS             4398..6239
                     /gene="ftsH"
                     /locus_tag="PKK95_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOC18552.1"
                     /translation="MSPRHWLARKPVIIGSAAACLFLVVSIALLRGTAADGAAAVAFS
                     DFLKQVESGRVTKVVIEDQSIRASLGDGREVVTLAPAGYAAAPSFMNELVSHRVQVVV
                     EPAAARRGVGYGALVMGLAVFGFIGFALYRLTAGRVPTLEKAHSSSSPETVVTFADVA
                     GVDEAKDEVREIVDFLRDPGRFTALGGRMPKGVLLVGPPGTGKTLLARSIAGEAGVPF
                     LFASGADFVEMYAGVGAARVRRLFKDAARHEKCIVFIDELDAVGRARGSNSLSHEERE
                     QTLNQLLVEMDGFATHRGIVVIAATNRPDILDQALLRPGRFDRQVTVGSPDLKGRIEI
                     LRVHTRKVPMAASVDLKAIARGTPGFSGAELANLVNEAALLAARSGRSEVTDADLDEA
                     RDKVLMGVERRSLVMTEKERINCAYHEAGHALVAAMLPNADPLHKVTIVPRGRALGVT
                     MQLPESDRHTHTAEDLETQVAILLGGRAAEELLMGHSTSGAANDIERATEIARKMVCD
                     LGMSPLGPLAFRMATGGWDQEAPSVHSEEMARRVDEEVRAIVMRGYDLARRIVNEHRA
                     AATAMAERLLVEESLDADAIREVLAGSVLPAARPEGLPQAVAGGAGL"
     gene            complement(6300..7841)
                     /locus_tag="PKK95_09805"
     CDS             complement(6300..7841)
                     /locus_tag="PKK95_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18553.1"
                     /translation="MKPLAPLLALGCVAAALAAGAAGQAPRPERLGVFSAHGDVGAVK
                     IAGSAAYDEAAGDYTLTASGANVWGTSDELHFAWREIEGDFILQARVNFVSVGELPSP
                     DAASKPAHNPHRKVGLMARSSLDPASPHVTVTAHGDGLTALQFRRVQKGETEEIRSDR
                     AGSDVLQLERRGNTWTALVARFGEPYAVSRVDDVKLGARAHVGIFLCSHEAEVVETAV
                     LHNVRLIRPAPEGFTPYRDYLGSLVEVLDVETGRRRVVHTVADSMQAPNWTNDGRRLV
                     MNRNGRIYSFELETRAVAEIDTAPMTRNNNDHALSFDGTMLGLSGGQPSTVWTVPAGG
                     GTARQITPAGPSYLHGWSADGKLLAFTGQREGDYDIYVVPAGGGPERRLTTAPGLDDG
                     PEFTPDGKWIYFNSTRSGRMQVWRMRPDGSGQEQLTFDDFNNWFPHVSPDGTRVVFIT
                     YGPEVAPDDHPWYKRVYLRTMPLDGGAPRVVAYVYGGQGSMNVNSWAPDSRRLAFVSN
                     SGELP"
BASE COUNT         1153 a         2795 c         2749 g         1290 t
ORIGIN      
        1 gtgaggcctt ccagcaggct ctggcttgtc gcagcgaaca cgccccggcc aggcctctcc
       61 gcacacatcg caccagccag aacctcgacg atctggaagc cgactacgag ctgggccacg
      121 agttggggaa ggcggcggtt cttcagctga ggggctgaca agcgtcagta gagaaggtac
      181 ttctgcctcg tctgttcgaa ctccgcggtc ggcccctccc actccgcggc aatgtccctg
      241 aaggagcggc ccgcctcgat cccctttcgc agtgcctccg acccggcgag gatgtcgatg
      301 ggcatcagcc ggtgctcgta ctcgtaggga ggctggcgcc acgcgaagcg ttccgggttc
      361 gcgtcgtgga aggcgccgag cacggcgacc gaggccagca cggggcggaa ggtccggcgg
      421 tcggtcacgt ggatctgcac gccgccgcac gcctggtgtg cgtgcttgtg gaacgtcggc
      481 tcgaagacga cggggcggaa gtgggccccc tcgatggcct gcgcgttcag cccggccgcg
      541 aagggctcgg cgtcgatcca cggggcgccg agaatctcga acggccgcgt ggtgcctcgc
      601 ccctccgaga gctgcgtgcc ctcgaacagc accgcacccg ggtagacgat ggccgtgtcg
      661 agcgtcggca tgttcggcga ggggatcacc cacggcagcc ccgtgtcgtc ccaatacatc
      721 ccgcgctgcc acccctccat cggcacgacc tccagatccg cgccgaggcc gtattcctcg
      781 ttgaagagac gggccagctc gccgacggtc atcccgtgcc gcatcgggat cgggaagagg
      841 ccgacgaacg actcgaaccc gcgctcgagc acgggccctt ccaccgcctc gcccccgatc
      901 gggttcggcc ggtcgcacac gacgacccgc acgccgtgcc gcgccgcggc cttcatgcag
      961 ttcgccatgg tgtagatgaa cgtgtagatc cgggccccca cgtcctggag gtcgatgacc
     1021 agcaggtcga ggccgtcgag catctcggcg gtcggctccc tcgtctcgct gtagagcgag
     1081 tacaccggta cgcggcgggc cgggtcgtgc ccgtgcggcg actcgatcat gttgtcctgc
     1141 acgtcggcgc ggaaaccgtg ctgcgggccg aagatcccgg caagctttgc gctggggtgc
     1201 gaggcgaggc ggtcgacgac gtggcggaac gagccgtcga tcgaggctgg gttcgcgacg
     1261 atgccaaccc ggcggttgtc gaggaggtgc gaggcgagaa ggcgctccga gccaaggcgg
     1321 acggtcatgc ccgacattat aggtccggcg cggccgcccc atcgctgaag cggccgcgtc
     1381 accgaaccct tacttctccc cctcggcctc ccgcttcttc ccggcttctt ccccgggcag
     1441 cgtatcgagc accatgcgcc aggagaactt ctcgccgaca ccgcgcacgt agtagtccat
     1501 ccacgccatc tcgctcaccg ccttcaccag ctggttccgc tggtcgggga tgccgtggct
     1561 cttgccgggg tagacgaaca gctcggtcgg cacgccgagg cgcttgagcg ccatgtgcag
     1621 ctccaccgac tgcgggctcg gcacgcgcgg gtcgccctcc accacgtgga tcatcgtcgg
     1681 cgtcttcgcg ttcctgacgt agcggatcgg cgactgcgcc cagtacggct cgaagtcgtc
     1741 gtagggcagc cggttgccca ggtagtactg ccggttgcgc tgcacgtcgc tctgggcgta
     1801 catcgagatc cagttcgatg tgccggcgcc cgagctgatg gccttgaaac ggtcggtgtg
     1861 ggtgagaatc cagttcgacc agtggccgcc ggcgctccag cccagggccc ccatcctcga
     1921 cccgtccacg attccctgcg cgatgaggtg atccacgccg gtcatgatgt cgtcgaaccc
     1981 gggcgggaag tagttgccga cgatcgcggt cttgtgcgcc tcgccgtagt tggtggatcc
     2041 ccggtagttc ggcatgagca cggcgtagcc ggcgccggcg tagacctggg agccgtagcc
     2101 gccgttgaag cggaggacgt cggccccggc cggcccgccg tggatggcga caatgagggg
     2161 gtagcgcttg cctttctcgt agcccacggg cttcacgagc accccgccga ccatccggcc
     2221 gtccgtcgac ttccaggtga tctcctcctg ctcaccgagc gcgagatcgc gcacctgcgg
     2281 gttcgactcg atgagcggcc gccaggcagc ccgggttccc gcctgctcca ccgacgcgac
     2341 ggtgtagacg ctcctcggcg tcgcctgatc ggagtagtcg atgagcagca cgcccgagtc
     2401 ctcgtcccgg tccacggcca atgccgcctt ctcgtacgtg atctgccgaa ccgtatcctt
     2461 gacgacgtcg agcgcgaaga gctgcgtggt ggccttgatc ccctggttga agtagatcgt
     2521 gccgccgtcc ttcgaccaga agccgatccc cacgtcgccg tcgaacgacg agccgagctt
     2581 gcgccacttg cccccgcggt cgccgaccgg gcgcaggtag acgcgccggt tcttcatgct
     2641 gtactgcgtg agatcgtccg gggccgagaa cgccatgtag cgcccgtcgg gtgagaagct
     2701 gggcagcccc tcgccgacct cttcgttggc gacgagccgc tcgatctgac ctgtcgcgac
     2761 ctcgagcagg tacgggtcgg cgtagatccc ctgctcggtg acgttgcgct tgtagcgctc
     2821 ggccgcgacc ccgctgaacg cgatccagcg cccgtcgtcc gacaccgtga agccggcgac
     2881 gctgtacgac gcgtcggccg tcaggcgttc gaccttcttc gactcgaggt ccagggccca
     2941 gaggctttcg atcggcgtct cggcgttgcg cacctcgacg gtgaacttct tctcgcgccg
     3001 ctgcttctcg tcggcgtcgc ggcgctccgg ggcgacgaag tagatgcggc ggccgtcggg
     3061 cgaccagcgc cacgcgccga cggccccttc gtgcctggtc agctgctccg ccttcgcgtc
     3121 ctcgatgccc ttcacgctca gccggtagag ctgctcctcg cccgcctttc cgctgcggta
     3181 gacgagccag gcgccgtcgc ggctgaaggc aaaggtcgag accccttcct tcgcgtccgt
     3241 gatcctccgt ccctcgccgc cgtcgatccg catcaggtag agctggttct gttccccctt
     3301 cgaggtgggg gcgtcgcggt tcgaggcgta gacgaagaac gagccgtcgc gcgcccaggt
     3361 gggctgggcc tcgttcttgt cgccgctgaa ggtgagctgg cgggtggagt cgatcccctt
     3421 ctcaatcgac accacgtaga tgtccgactg ccgccgcgct tccttccagt cgggcttgtt
     3481 cagcgtgtag agcagccagc gcccgtcagg gctcgagcgc ggcgcgccga cctgctccag
     3541 gttttgcacg tcgaggaagg tcatcgggcg tttcgccggc gttccctgtc ctctggccgg
     3601 gggcgagacg gcaagtgagc cgactacgag cagggcggcg accaggcaca tcgaacggac
     3661 gggaccggcg ctgggcatgc gtgctccttc tgcgaggcga gtgctgaaac ttgccgggcc
     3721 tgtcaggtga gacgggccgg cgaggtgagt ttgccccggc gggaccgccc gggtccagcg
     3781 tgtctggaac ggtccctgga acggggtttg cggtggcgcc tgctcgtgct agcatgagtg
     3841 gttgaccacc ccccgcgctc cacgagcccg gggagaccta gccaaaagga cagagccatt
     3901 tgagcaaaga agacctcctc gacattcccg gaaccgtcac ctccatccac ggcggcggcc
     3961 tctaccgcgt gcaggcggac gcgggccagg agatcctggc gcaattgagc ggccgcatgc
     4021 gtcgcttccg catcaaggtg gtcaccggcg accgcgtgac cgtcagcgtc tccccgtacg
     4081 acatggtgcg gggcatcatc accttccgct tccgttgaga cgacgagccg gcgacgggcg
     4141 ccccgctacc cgcgtgttcg ggcgccccgg cctgccccgc agaaggggtc gccttgcgac
     4201 ctgaacctca gctgtatcaa gcatttacag gaccggcccc ggtcgtgtag gatggggctt
     4261 gcttcggagc ggccgaacgc cggctcctcc ccgcactcat actctcaaac acacgcgcac
     4321 ggccggccgg cggccgtgtt tgtctgctgc cggcgacccc gtccgccgcg cgaaggggcc
     4381 cggctcacgg agtgaccgtg tcgccacgcc actggctggc ccgcaagccc gtcatcatcg
     4441 gttcggctgc cgcttgtctc ttccttgtcg tttcaatcgc cctgctccgc gggaccgccg
     4501 cggacggggc ggccgccgtc gccttctcgg atttcctcaa acaggtcgag tcggggcgcg
     4561 tcaccaaggt cgtcatcgag gaccagtcga tacgggcctc gctcggcgac gggcgcgagg
     4621 tcgtcaccct cgcgccggcc ggctacgccg ccgcgccctc cttcatgaac gagctggtct
     4681 cgcaccgcgt ccaggtagtc gtcgaaccgg ccgccgcgcg gcgcggggtt ggctacggcg
     4741 cgctcgtcat ggggctcgcc gtcttcggct tcatcggctt cgccctgtat cgcctcacgg
     4801 ccggccgcgt gccgacgctc gagaaggcgc actcctcgtc gtcgcctgaa acggtggtga
     4861 ccttcgccga cgtggccggg gtggacgagg cgaaggacga ggtccgggag atcgtggact
     4921 tcctccgcga ccccgggcgg ttcaccgcgc ttggcggccg catgccgaag ggggtgctgc
     4981 tggtggggcc gcccgggacc ggcaagacgc tcctggcgcg gtcgattgcc ggcgaggcgg
     5041 gcgtgccgtt cctcttcgcg agcggcgccg acttcgtgga gatgtacgcg ggggtgggcg
     5101 ccgcccgcgt ccgccggctg ttcaaggacg ccgcccgcca cgagaagtgc atcgtcttca
     5161 tcgacgagct cgacgcggtc ggccgcgcgc gcggcagcaa ctcgctcagc cacgaggagc
     5221 gcgagcagac cctcaaccag ctgctggtcg agatggacgg cttcgcgacg caccggggga
     5281 tcgtcgtgat tgccgcgacc aaccgtcccg acatcctcga ccaggcgctg ctgcgtcccg
     5341 gacgcttcga ccgccaggtg accgtcgggt cgcccgattt gaaggggcgg atcgagatcc
     5401 tgcgggtgca cacgcgaaag gtgccgatgg ccgcgtcggt cgatctcaag gcgattgccc
     5461 gcggcacgcc ggggttctct ggggccgagc tggcgaacct ggtgaacgag gcggccctgc
     5521 tggcggcgcg gtcggggcgg tcggaagtga ccgacgccga cctcgacgag gcgcgcgaca
     5581 aggtgctgat gggggtggag cggcgctcgc tcgtgatgac cgagaaggag cgcatcaact
     5641 gcgcctatca cgaggcgggg cacgcgctgg tggccgcgat gctgccgaac gccgacccgc
     5701 tgcacaaggt gacgatcgtt ccccgcgggc gcgcgctcgg cgtcacgatg caactgcccg
     5761 agtcagaccg ccacacgcac accgccgagg acctcgagac gcaggtggcg atcctgctgg
     5821 gcggccgcgc ggccgaggag ctgctgatgg ggcactccac cagcggcgcc gccaacgaca
     5881 tcgagcgcgc caccgagatc gcgcgcaaga tggtctgcga cctcggcatg tcgccgctcg
     5941 ggccgctcgc gttccgcatg gcgaccgggg gctgggatca ggaggcgccg tcggtgcaca
     6001 gcgaagagat ggcgcgccgc gtggacgagg aggtgcgggc gatcgtgatg cgcggatacg
     6061 atctcgcccg ccgcattgtc aacgagcacc gggcagccgc caccgcgatg gccgagcgcc
     6121 tgctcgtcga agagtcgctc gacgccgacg ccatccgcga ggtgctcgcc gggagcgtgc
     6181 tgccggcagc caggcccgaa ggcctgcccc aggccgtcgc cggcggggca ggactctaga
     6241 ggttgacggg tagcgcaggg ctcaagccct gcgctacggg ggcagggccg aagcctgccc
     6301 tacgggagct ctccgctgtt gctgacgaag gccagccgcc ggctgtcggg ggcccaagag
     6361 ttcacgttca tcgacccctg cccgccgtac acgtacgcca ccacccgggg cgcgccgcca
     6421 tcgagcggca tggttctcag gtagacgcgc ttgtaccagg ggtggtcgtc gggtgctacc
     6481 tcgggaccgt acgtgatgaa gacgacccgc gtcccgtcgg gcgacacgtg cgggaaccag
     6541 ttgttgaagt cgtcgaaggt cagctgctcc tgcccgctgc cgtccggccg catgcgccac
     6601 acctgcatcc ggccgctcct cgtcgagttg aagtagatcc acttcccgtc cggcgtgaac
     6661 tcggggccgt cgtccaggcc gggggccgtc gtcaggcgcc gttcggggcc gcccccggcc
     6721 ggcacgacgt agatgtcgta gtcgccctcg cgctggccgg tgaaggccag cagtttcccg
     6781 tccgccgacc atccatgcag ataggagggc ccggccgggg tgatctgccg ggccgtcccc
     6841 ccgcccgccg gcacggtcca caccgtcgag ggctggcccc cgctcagccc cagcatcgtg
     6901 ccgtcgaacg acagcgcatg atcgttgttg ttgcgcgtca tcggcgccgt gtcgatctcg
     6961 gccaccgcgc gcgtttccag ctcgaacgag tagatccggc cgttccggtt catcaccagc
     7021 cgccgcccgt cgttcgtcca gttcggcgcc tgcatcgagt ctgcgaccgt gtgaaccacg
     7081 cgacggcggc ctgtctcgac gtcgagcacc tccaccaggc tcccgaggta atcgcggtac
     7141 ggcgtgaatc cttccggcgc cggcctgatg agccggacgt tgtgcaggac agccgtctcc
     7201 accacctccg cctcgtgcga gcagaggaag attcccacgt gcgcccgggc cccgagcttc
     7261 acgtcgtcaa cccggctgac cgcgtacggc tcgccgaagc gggcgaccag agccgtccag
     7321 gtgttgccgc gccgttcgag ctgcagcacg tccgaccccg cgcggtcgga cctgatctcc
     7381 tcggtctctc ccttctggac ccgccggaac tgcagggcgg tgaggccgtc gccgtgggcc
     7441 gtcaccgtaa cgtgcggcga ggccgggtcg aggctcgagc gcgccatcaa tccgaccttg
     7501 cggtgcgggt tgtgggcggg cttggatgcc gcgtcaggcg aggggagctc tcccacagag
     7561 acgaaattca cgcgggcctg aaggatgaag tcgccctcga tctccctcca cgcgaagtgc
     7621 agttcgtcac tggtgcccca gacgttggcc cccgaggccg tcagcgtgta gtccccggcg
     7681 gcttcgtcgt aggcggcgct gccggcaatc ttcaccgcgc cgacgtcccc gtgagcgctg
     7741 aacacgccaa ggcgttccgg gcgaggggcc tggcccgccg cgccggcggc gagggccgcc
     7801 gcgacgcagc cgagggcgag tagcggggcg agaggcttca tggtcgtctc cggggaagaa
     7861 accgccttcg tttcgcggca gaccgctcac agcgagaacc agtagctggc cttgacgagc
     7921 aggacgttgt cagcccggtc gcgccacagc tggttccagt ggccgccgaa cgggcgggtc
     7981 gggtctg
//
LOCUS       DAOPQX010000164         7983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10118_length_7983_cov_4.028633, whole genome shotgun sequence.
ACCESSION   DAOPQX010000164 DAOPQX010000000
VERSION     DAOPQX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10118_length_7983_cov_4.028633"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PKK95_09810"
     CDS             complement(<1..608)
                     /locus_tag="PKK95_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18554.1"
                     /translation="MDHPAPAPSEAASTTSAVMVGVACVSSSLLMTELALTRIFSVTM
                     YYHFAFLAISIALFGLSASGVYVFLARKRLARVPARTLLAWHALVYAVVTTVSLASLV
                     RIRVGLNYSPRNLALMLAIYALAALPFLAGGSVITLAISRLAGRINLVYAVDLIGASL
                     GGVLLLPLLDRIGAPGVVLATASLASLAAACFAPPAPRRRVVL"
     gene            687..1652
                     /locus_tag="PKK95_09815"
     CDS             687..1652
                     /locus_tag="PKK95_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC18555.1"
                     /translation="MTVLVPVLAAGMLALLGAQGPPAAPSATGQGVFRAATELVTLGV
                     TVTDAADRQVTGLGPDDFVVLENGKPQSIKFFSAGEAPLDVVLLVDTSSSMADKLEPS
                     RQAAKRFLATLRPSDRASIVEFNTQVRVLHGFTSDRDLLERAIDATHAGGGTALYTGL
                     YVALDHFARLDRAGDGVRKSAIVVLSDGQDTASLLGEEEVTERARRAGVPLYFISLLS
                     AQEEQALAVARTRRRSTPHDFVLNALARETGARAYFPGRLEELEGVYETVANELAQQY
                     MLAYAPGDVAADGSFRRILVRVPGHPGARPRTRAGYYAPGEHARR"
     gene            1715..2203
                     /locus_tag="PKK95_09820"
     CDS             1715..2203
                     /locus_tag="PKK95_09820"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOC18556.1"
                     /translation="MRSSHLLAVIRAALVSAALLWSGLILAAPWIATSPDRIPSHLAA
                     GTYVVGAAVCHQRPERSFHISSVRLPVCARCTGLYLSAALGAIAGLAWAGSREKHPRR
                     NQRDWRSWLAAAAVPTALTLVLEWLGLWAPSNAARALAAAPLGVVGGLLVAVGLSFRS
                     KL"
     gene            2206..2598
                     /locus_tag="PKK95_09825"
     CDS             2206..2598
                     /locus_tag="PKK95_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18557.1"
                     /translation="MRAEATTRAPSTSVVLVGLAAWLVPGGGHLWMGRRLKGLVFLGA
                     LVLMFALGLSLQGRLFPFEFAQPLVGLAAFANLAAGLPYFVAWAAGLGDGQVIAATYE
                     YGNAFLIAAGLLNTLVVLDACDIAAGRK"
     gene            2616..2789
                     /locus_tag="PKK95_09830"
     CDS             2616..2789
                     /locus_tag="PKK95_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18558.1"
                     /translation="MTSHLLYMTLFAFLVSLVFAVLLKDEPRQQLRFGVLTFAGFMLG
                     ALALGWLMYPFPL"
     gene            2803..3231
                     /locus_tag="PKK95_09835"
     CDS             2803..3231
                     /locus_tag="PKK95_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOC18559.1"
                     /translation="MTLPAPPVPDRRPPRPARWLRLWGPVVLYGILIFSLSSASNLPD
                     LPKGFTDKVAHLLLYCGLGFLLARALAGGLGQRVPAWVPPLVVLLSVAYGVSDEVHQF
                     FVPNRQFDLLDLAADAAGAALGAGALWLWGIIARPGDALR"
     gene            3218..4000
                     /locus_tag="PKK95_09840"
     CDS             3218..4000
                     /locus_tag="PKK95_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOC18560.1"
                     /translation="MPSDSLSIGTDGPIAIVTLNRPQVRNALDAATLAALEEVLLRLK
                     QDEGARSVIVTGAGDRAFVAGADIGELSRLSGTEARAYALAGQRVFDLIEHLGKPVIA
                     AVNGRALGGGCELALACHLRVASDTAAFGQPEVKLGLIPGFGGTQRLPRLIGKSRALE
                     LLLTGREVGADEALRVGLVHRVVPAAELMPACRAWALELSALPPLALANVLDAVARGM
                     EMSFPEAAFLEAALFGAVAGSEDAREGTRAFLEKRRPAFKGR"
     gene            4002..4457
                     /gene="nusB"
                     /locus_tag="PKK95_09845"
     CDS             4002..4457
                     /gene="nusB"
                     /locus_tag="PKK95_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOC18561.1"
                     /translation="MSGERAEWRRERRRAREAALQMLYQCEVGGMAPPEAEAAYRQID
                     GLGPVLPAESAAFAAALVEGTVANLARIDPLIEESAEHWRLSRMAVVDRLVLRLAVFQ
                     LLHLPEVPGAVVIDEAVDIAALYGGPESGRFVNGVLDAIHRRLKTTPEG"
     gene            4483..5985
                     /gene="lysS"
                     /locus_tag="PKK95_09850"
     CDS             4483..5985
                     /gene="lysS"
                     /locus_tag="PKK95_09850"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOC18562.1"
                     /translation="MPQEPELLAQRRENMEALARLGVDLYPHEFARTHTVHELVSAFG
                     ARSRDQLESERIEATTSGRILAIRSFGKANFLVISDGRSRLQVYLRQDSLPARDFQVA
                     RLLDLGDFVGVSGHLFRTRTDEFTLWASSLTFLAKCLVPLPEKWHGLQDIEIRYRQRY
                     LDLIVNPDSRRVFEARSATLAALRAFLDARGYLEVETPMMQVLAGGALARPFVTHHFA
                     LDMKLYLRIAPELYLKRLLVGGLERVYEINRNFRNEGISTQHNPEFTMLEFYQSYSHY
                     LELMDMTEQMLSEVALKVTGGDEVPFGDHRLSFRPPYRRLSMRPAVCEEASRRLGRPV
                     EERECRDLEAARALARALGVPIEPAMGPGKITAEVFEALWEPQLIQPTFVHDFPTEVS
                     PLSKQRPDDPDTVERFELYAGGFELANAFSELNDPAEQRRRFEEQLRQREGGDEEAHQ
                     MDEDYVLALEHGMPPAGGEGVGVDRLVMLLTDSRSIRDVILFPLMRPRKE"
     gene            5982..7214
                     /locus_tag="PKK95_09855"
     CDS             5982..7214
                     /locus_tag="PKK95_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18563.1"
                     /translation="MTLPFELQIALRYLLARRKQAFISVISAISTLGVIVGVMALVIA
                     LALMTGLQRELRDRILGSTAHVYVFGVGGGIRDYRADVERLRQVPHVTGAAPAVLGQA
                     LMTTEQAQAFVTIKGIDFELEPTVTDIGRSMRSGSLAALEADGDRVPGVLLGVDLAKK
                     LGVGVGDFVQVLTPEGTLTPFMGMMPRTRRLAVAGTFSLGLFEFDSAYGFVALSVAQR
                     LTGRDHPELIELRVDDIYRAPEVAEAISRQYPTRYVTEDWTSMNRSLFSALWLEKMAI
                     SITIGLIVMVAALNIVASLILLVMEKHRDIAILKTMGASGRSLMAVFMAQGLVIGLVG
                     TLLGSAAGLGVSFVLDHYKLIRIPMDVYQVSYVPFVVLWWDFALVVVSAIVICFVATI
                     YPSRQASKLDPAEALRYG"
     gene            7250..7960
                     /locus_tag="PKK95_09860"
     CDS             7250..7960
                     /locus_tag="PKK95_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18564.1"
                     /translation="MESGERREGGGEAMPFIEVRGLDKRYRSATGELQVLRGLDLEVP
                     RGQMVAIVGASGVGKSTLLHVLGGLDRFDAGSIRIGDADLGAMADADVVAFRNRNVGF
                     VFQFHHLLPEFNALENAAMPLRIARLPIAEAYPRAAALLERVGLGDRLTHRPGTLSGG
                     EQQRVAIARALVMPPSLLLADEPTGDLDEHTADSLHDLLRQMHREQGLTAVIATHNLR
                     LAAICDRVLRLEDGRLHG"
BASE COUNT         1075 a         2764 c         2904 g         1240 t
ORIGIN      
        1 aggaccacgc gccgcctcgg cgcgggcggc gcgaaacagg cggccgccag cgaggcgagc
       61 gacgcggtgg cgagtaccac gcccggggcg ccgatccggt cgagcagcgg cagcagcagc
      121 acgccgccca gcgaggcgcc gatcaggtcc accgcgtaga ccaggttgat gcggccggcc
      181 agccgggaga tcgccagcgt gatgaccgag ccgcccgcca ggaagggcag ggccgccagc
      241 gcgtaaatcg ccagcatcag cgccaggttt cgcggcgaat agttgagccc gacgcggatg
      301 cggacgagcg acgccagcga cacggttgtc accaccgcat acacgagcgc gtgccacgcg
      361 agcagcgtgc gcgccggcac ccgggcgagc cgcttccggg ccaggaacac gtacacgccg
      421 ctggcgctga ggccgaacag cgcgatcgaa atcgccagga acgcgaagtg gtagtacatc
      481 gtgaccgaga agatgcgcgt gagcgccagc tcggtcatca gcagggagct cgacacgcac
      541 gccaccccca ccattacggc cgacgtggtg gaggcggcct cggaaggggc gggcgccggg
      601 tgatccatcc gggcagtcta ccaccggaaa ggaacgtcgt ttgctaacgc ggtcacgaca
      661 gcggcagtga ctggagggct ttcgaagtga ccgtgctggt cccggtgctt gcggcgggca
      721 tgttggcgtt gctgggcgcg caggggccgc ccgcggcgcc gtcagccacc ggtcagggtg
      781 tgtttcgggc ggccaccgag ctggtgacgc tgggcgtcac ggtgacggac gcggcggacc
      841 gccaggtgac agggctcgga ccggacgact tcgtcgtgtt ggagaacggc aagccgcaga
      901 gcatcaagtt cttctctgcc ggtgaggccc cgctcgacgt cgtcctgctc gtcgacacca
      961 gctcgagcat ggccgacaag ctcgagccgt cgcggcaggc cgccaagcgg tttctcgcga
     1021 ctctgcgccc gtccgaccgc gcctccatcg tcgagttcaa cacgcaggtc cgggtcctcc
     1081 acgggttcac cagcgaccgc gacctgctcg aacgggccat cgacgccacg catgccggag
     1141 gcggcaccgc cttgtatacg ggcctttacg tcgccctcga tcacttcgcc cggctcgacc
     1201 gggcggggga cggcgtgcgc aagtcggcca tcgtggttct cagcgacggc caggacaccg
     1261 ccagcctgct cggcgaggaa gaggtgaccg agcgggcgcg ccgcgccggc gtgcccctct
     1321 acttcatctc cctgctgtcg gcccaggagg agcaggcgct ggccgtggcg cgcacccggc
     1381 ggcgttcgac gccccacgac ttcgtgctca acgcgctcgc cagggagacc ggggcgcgcg
     1441 cctacttccc cggccgcctc gaagagctgg agggggtgta cgagaccgtg gccaacgagc
     1501 tggcccagca gtacatgctc gcgtacgcac ccggggacgt ggccgccgac gggagcttca
     1561 ggcggatcct ggtgcgggtg ccgggtcatc ccggggcacg gccgcggacg cgtgccggct
     1621 actacgcgcc cggggaacac gcgcgacggt gagagcaggg cggaacatga cggtcgagtg
     1681 acgaatggag gatggacacg tagaatgaag gtctttgagg tcctcccacc tgcttgccgt
     1741 cattcgggcc gcgctcgtgt cggcagcgtt gctgtggagc gggctgattc tggccgcccc
     1801 gtggatcgcg acgtctcctg accggatccc gtcccatctt gccgcgggca cctacgtggt
     1861 cggcgccgcg gtgtgccacc agcggcccga gcgcagcttc cacatttcct cggttcgcct
     1921 gcccgtctgc gcccgctgca cggggctcta cctttcggcg gcgctgggag cgatcgcggg
     1981 gctggcctgg gccggttccc gagagaagca cccgcggcgc aatcagcgcg actggcgctc
     2041 ctggctcgcc gccgcggcgg tgcccacggc cctgacgctg gttctggagt ggctgggtct
     2101 ctgggcgccg tcgaatgccg cgcgcgccct cgccgccgcg ccgctggggg tggtgggggg
     2161 cctgctggtg gcggtcgggc tgagctttcg gagtaaactg taacgatgcg tgcggaagcc
     2221 accacccgcg ccccctcgac ctcggttgtg ctcgtcggcc tggcggcctg gctggtcccg
     2281 ggcgggggcc acctgtggat ggggcggcgg ttaaaggggc tggtgttcct gggcgcgctc
     2341 gtcctgatgt tcgccctcgg gttgtcgctg caggggcggc tgtttccctt cgagttcgcc
     2401 cagccgctgg tcgggctggc ggcgttcgcg aacctggctg cggggcttcc ctacttcgtc
     2461 gcgtgggcgg ccggcctcgg cgatgggcag gtgatcgcgg ccacttacga gtacggaaat
     2521 gcctttctca ttgcggcggg cctgctgaat accctcgtgg tgctcgacgc ctgcgacatc
     2581 gccgcggggc gcaagtagtt ctggggagcc gtgacgtgac gagccacctc ctctacatga
     2641 cgttgttcgc gttcctggtg tcgctcgtgt tcgcggtcct cctcaaggac gagccgcggc
     2701 agcagctccg cttcggcgtg ctgaccttcg cgggcttcat gctcggcgcg ctcgcgctcg
     2761 gctggctgat gtaccccttc ccgctgtgat cgcgaccgac cggtgaccct tccagctccc
     2821 cccgtccccg atcgccggcc tccgcgcccc gcccgctggc tgcggctctg ggggcccgtg
     2881 gtgctgtacg ggatcctgat cttcagcctc tcgtcggcgt cgaacctgcc cgacctgccg
     2941 aaggggttca ccgacaaggt ggctcacctg ctgctctact gcggtctggg cttcctcctg
     3001 gcgcgcgcgc tggcgggcgg cctcgggcag agggtccccg cctgggtgcc gccgctcgtc
     3061 gtgctgctgt ccgtggcgta cggcgtgagc gacgaggtgc accagttctt cgtgccgaac
     3121 cggcagttcg acctgctcga cctcgcggcc gacgcggcgg gtgccgcgct cggggcgggc
     3181 gcgttgtggc tgtggggtat aatcgcgagg cccggcgatg ccctcagata gcctctccat
     3241 cggcacggac ggcccgatcg cgatcgtcac cctcaatcgc ccccaggtcc ggaacgccct
     3301 cgacgccgcg acgctcgccg ccctcgagga ggtcctgctg cggctgaagc aggacgaggg
     3361 ggcgcgatcg gtgatcgtga ccggcgcggg cgatcgcgcg ttcgtggcgg gcgccgacat
     3421 cggcgagttg tcgcggctct cgggcaccga ggcccgggcg tacgcgctgg cgggccagcg
     3481 cgtgttcgac ctgatcgagc acctcggcaa gccggtgatt gccgccgtca atgggcgcgc
     3541 gctgggcgga ggctgcgagc tggcgctcgc gtgccacctg cgggtagcct ccgacacggc
     3601 cgcgttcggg cagcccgagg tgaagctggg gctcatcccg ggcttcggcg gcacgcagcg
     3661 gctgccccgg ctgatcggga agagccgggc actcgagctg ctgctcacgg gccgcgaggt
     3721 gggcgccgac gaggcgctcc gcgtcgggct ggtgcaccgc gtcgtccccg cggcggagct
     3781 gatgcccgcg tgccgggcgt gggcgctcga gctctcggcg cttccgccgc tggccctggc
     3841 caacgtactg gacgcggtcg cgcgcgggat ggagatgtcg tttcccgagg cggccttcct
     3901 cgaggcggcg ctcttcggcg cggtcgccgg ttccgaagac gcgcgcgagg ggacgcgcgc
     3961 gtttctcgag aagcgccgcc cggcgttcaa ggggcggtga ggtgagcggc gaacgggcgg
     4021 agtggcgccg cgaacggcgg cgcgcgcgcg aggcggcgct gcagatgctc taccagtgcg
     4081 aggtcggcgg gatggcgccc ccggaggccg aggccgctta ccggcagatc gacgggctgg
     4141 ggccggtgct cccggccgaa tcggccgcct ttgccgccgc gctggtcgag gggacggtgg
     4201 cgaacctcgc ccggatcgac ccgctcatcg aagagagcgc cgagcactgg cggctgtcgc
     4261 gcatggcggt ggtcgatcgc ctggtgctgc ggctcgcggt cttccagctg ctgcacctgc
     4321 cggaggtgcc gggcgcggtc gtcatcgacg aggcggtgga catcgcggcc ctctacgggg
     4381 gccccgagtc gggccggttc gtcaacggcg tcctggacgc gattcaccgg cgcctcaaga
     4441 cgacccccga aggttgatcc ccgttgcccc cgtgctgacc tgatgccgca agaaccagaa
     4501 ctgctggccc agcgccgcga gaacatggag gcgctcgcgc gcctcggcgt cgatctctac
     4561 ccgcacgagt tcgcccggac gcacacggtg cacgagctgg tgagcgcctt cggcgcgcgc
     4621 tcgcgcgacc agctcgagtc ggagcggatc gaggcgacca ccagcggccg catcctcgcc
     4681 atccgcagct tcgggaaggc gaacttcctg gtcatctccg acgggcgctc gcgcctgcag
     4741 gtgtacctgc ggcaggactc gctgccggcg cgcgacttcc aggtggcgcg cctgctcgac
     4801 ctgggcgact tcgtcggcgt ttccgggcac ctgttccgca cgcggaccga cgagttcacc
     4861 ctgtgggcgt cgagcctcac ctttctcgcc aagtgcctcg tgccgctgcc cgagaagtgg
     4921 cacggcctcc aggacatcga gatccgctac cggcagcgct acctcgacct gatcgtcaac
     4981 cccgactcgc ggcgggtgtt cgaggcgcgc agcgcgaccc tggcggccct gcgcgcgttc
     5041 ctcgacgccc gcgggtacct cgaggtcgag accccgatga tgcaggtgct cgccggcggc
     5101 gcgctggcgc gcccgttcgt cacgcaccac ttcgcgctcg acatgaagct gtacctccgc
     5161 atcgcgccgg agctctacct gaagcggctg ctggtcggcg gcctcgagcg cgtctacgag
     5221 atcaaccgca acttccggaa cgaggggatc tccacccagc acaaccccga gttcacgatg
     5281 ctcgagttct accagtccta cagccactac ctcgagctga tggacatgac cgagcagatg
     5341 ctctccgagg tggccctcaa ggtgaccggc ggcgacgagg tccccttcgg cgaccatcgc
     5401 ctctcgttcc gcccgccgta ccggcggctg tcgatgcgcc cggcggtgtg cgaggaggcg
     5461 tcgcggcggc tgggccggcc cgtcgaggaa cgcgagtgcc gcgacctcga ggcggcacgc
     5521 gcgcttgcgc gggcgctcgg cgtgcccatc gagccggcga tggggcccgg caagatcacc
     5581 gcggaggtct tcgaggcgct ctgggagccg cagctcatcc agccgacctt cgtccacgac
     5641 ttcccgaccg aggtctcgcc cctctcgaag cagcggccgg acgatcccga cacggtggag
     5701 cggttcgagc tgtacgcggg gggcttcgag ctggccaacg ccttcagcga gctgaacgac
     5761 ccggccgagc agcggcgccg gttcgaggag cagctgcgcc agcgcgaggg cggcgacgag
     5821 gaggcccacc agatggacga ggactacgtc ctcgccctcg agcacggcat gcccccggcg
     5881 ggcggcgagg gggtcggtgt ggacaggctg gtcatgctgc tcaccgacag ccgctcgatc
     5941 cgcgacgtga tcctgtttcc gctgatgcgg ccgagaaagg aatgaccctc cccttcgagc
     6001 tgcagatcgc cctgcgctac ctcctggccc ggcggaagca ggcgttcatc tccgtcatct
     6061 cggccatctc gacgctcggc gtcatcgtgg gcgtgatggc gctggtgatc gcgctggccc
     6121 tgatgaccgg cctgcagcgc gagctgcgcg atcgcatcct cggatcgacg gcccacgtgt
     6181 acgtcttcgg cgtcggcggc ggcatccgcg actaccgcgc cgacgtcgag cgcctgcggc
     6241 aggtgccgca cgtgaccggc gccgcaccgg cggtgctcgg ccaggcgttg atgacgaccg
     6301 agcaggcgca ggccttcgtg accatcaagg gcatcgactt cgagctcgag ccgacggtca
     6361 ccgacatcgg ccggtcgatg cggtcgggca gcctggccgc cctcgaggcc gacggcgaca
     6421 gggttcccgg cgtgctgctc ggcgtggacc tggcgaagaa gctgggggtg ggggtcggcg
     6481 acttcgtcca ggtgctgacg ccggagggga cgctgacgcc cttcatgggg atgatgccgc
     6541 ggacgcgccg gctcgcggtt gccggcacct tctcgctcgg gctcttcgag ttcgactcgg
     6601 cgtacggctt cgtcgcgctg agcgtggcgc agcggctcac gggccgcgac caccccgagc
     6661 tgatcgagct gcgcgtggac gacatctacc gggcgcccga ggtggccgag gcgatctcgc
     6721 ggcagtaccc gacccgctac gtcaccgagg actggacctc gatgaaccgg tcgctcttct
     6781 cggcgctctg gctcgagaag atggccatct ccatcaccat cggcctgatc gtcatggtgg
     6841 cggcgctcaa catcgtggcg tcgctgatcc tgctggtgat ggagaagcat cgcgacatcg
     6901 ccatcctcaa gaccatgggg gcgtcgggcc ggagcctgat ggcggtcttc atggcccagg
     6961 ggctggtgat cggcctggtc gggacgctgc tcggctcggc ggcgggcctg ggcgtgtcgt
     7021 tcgtcctcga ccactacaag ctgatcagga tcccgatgga cgtctaccag gtctcctacg
     7081 tgccgttcgt cgtgctgtgg tgggacttcg ccctggtggt cgtctcggcc atcgtcatct
     7141 gcttcgtcgc gacgatctat ccctcgcggc aggcgtcgaa gctcgacccg gccgaggcgc
     7201 tgcggtacgg ataggcgcgg gcggaggacg gcggggggag gtctgcagag tggagagcgg
     7261 agagcggagg gagggcggcg gggaggcgat gccgttcatc gaggtgcgcg ggctcgacaa
     7321 gcgctaccgg tcggccaccg gcgagctgca ggtgctgcgg gggctggacc tcgaggtgcc
     7381 tcgcggccag atggtggcca tcgtcggcgc gtcgggcgtg ggcaagagca cgctgctgca
     7441 cgtcctcggc gggctcgacc gcttcgacgc ggggagcatc cgcatcggcg acgccgatct
     7501 cggcgcgatg gccgacgccg acgtggtggc gttccggaac cgcaacgtgg ggttcgtttt
     7561 ccagttccac cacctgcttc ccgagttcaa cgccctcgag aacgccgcga tgccgctgcg
     7621 gatcgcgcgg cttccaatcg cggaggcgta cccgagggcc gcggcgctgc tcgagcgcgt
     7681 cggcctcggc gaccgcctca cccaccggcc cggcacgctg tcggggggcg aacagcagcg
     7741 ggtggcgatt gcccgcgcgc tggtcatgcc tccctcgctg ctgctggcgg acgagccgac
     7801 cggcgacctc gacgagcaca cggcggactc cctccacgac ctgctccgcc agatgcaccg
     7861 cgagcagggg ctgaccgccg tgatcgccac ccacaacctg cgcctggccg ccatctgcga
     7921 ccgtgtcctc cgtttggagg acgggcgtct ccacgggtga caggagaagg ctcagggctc
     7981 atg
//
LOCUS       DAOPQX010000165         7962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10159_length_7962_cov_3.963703, whole genome shotgun sequence.
ACCESSION   DAOPQX010000165 DAOPQX010000000
VERSION     DAOPQX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_7962_cov_3.963703"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKK95_09865"
     CDS             <1..197
                     /locus_tag="PKK95_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18565.1"
                     /translation="VFAALSGIPLHLVSRAPGSRHIAFVLADADLPTATARLHHAFLS
                     APVWHHSPAALRVAAAGGAR"
     gene            257..1579
                     /locus_tag="PKK95_09870"
     CDS             257..1579
                     /locus_tag="PKK95_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOC18566.1"
                     /translation="MSRHGSFGPATTALHGGHTPDPATGACAVPIYQTASYAFESVDQ
                     AAALFSLEKPGYIYTRIGNPTTDVLERRLAEMEGGVGALALASGQAAITAAVLTVARA
                     GDNIVSTASLYGGTVNLFHHTLARMGIEARFVDTSDPARVAAAIDDRTRLVYTESIGN
                     PRNNVDDLEALAGTAHAAGVPFVVDNTVTPHLLRPFDHGADLIVYSLTKFVGGHGTSI
                     GGALVDSGRFDWANGRFPEFTSPDPSYHGLVFWDAFGLHDRAVLRGAAFITKARVQVL
                     RDLGACLSPFNAFLFLQGLETLPYRMAAHASNARRVAEWLSVHPDVAWVNYPLLPEHP
                     DHERARRYFPAGAGAIVGFGIRGGREAAERFISGVRLFSHLANIGDAKSLVIHPASTT
                     HQQLTPEQRKAAGVTDDFIRLSVGIEDAGDLIADLEQAIARARKVTAA"
     gene            1576..2793
                     /locus_tag="PKK95_09875"
     CDS             1576..2793
                     /locus_tag="PKK95_09875"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOC18567.1"
                     /translation="MNDEGEGSVGQVETRIVEVCTGGLTLESGRRLESVEVAYEQYGE
                     IDAERDNVVLVCHALSGGAHAAGWHPGARKPGWWDNLIGPGKAFDTRKYCVICSNVLG
                     SCYGTTGPSSAEPATGRPYGSRFPVVTVGDMVTVQKALLDRLGIRRLLAVVGGSMGGM
                     QALQWAVACPASVRSVIAIASSARHSPQQIAFNEIARRAVTADPAWSGGDYYGSERPD
                     RGLAVARMIGHVTYLSAGGMERKFGRRAAAPAPSWTLEPEFEVERYLSHQGREFADRF
                     DANSLVYLTRAIDYFDLGAGWPSLTEAFRAARASFLLLTFSSDWLYPPSQLAEVGRAA
                     SDAGRAVEYHEIPSGYGHDAFLLESEAQTPIIRSWLERVAEASRQPRVSMPALARSRS
                     SSCIPAGAGGRGG"
     gene            2786..3358
                     /locus_tag="PKK95_09880"
     CDS             2786..3358
                     /locus_tag="PKK95_09880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HOC18568.1"
                     /translation="MVERPPATEREKMLAGQLYDPFDPDLVAGRERARDLCQALNSSG
                     ERRQEERRRILRELFAKGGESVWMQPPFFCDYGVNIELGERVFFNFNCVVLDVCRVRI
                     GDCTLFGPAVQIYTPMHPWNAGQRRVSEFGKPVEIGSDVWVGGGAIILPGVKIGSRTV
                     IGAGSVVTRDLPGDVFAAGNPCRVIREIRE"
     gene            complement(3446..3919)
                     /locus_tag="PKK95_09885"
     CDS             complement(3446..3919)
                     /locus_tag="PKK95_09885"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC18569.1"
                     /translation="MAKNTICLWYDKDAEAAARFYTATFPDSRMGAILRAPADFPGGK
                     KGDVLTVEFVVAGVACVGLNGGPVFQHNEAFSFQIATEDQEETDRYWNAIVGNGGQES
                     ACGWCKDRWGVSWQITPRVLTEALAAGGDEARRAFEAMMPMRKIDVATIERARRG"
     gene            complement(4006..5100)
                     /locus_tag="PKK95_09890"
     CDS             complement(4006..5100)
                     /locus_tag="PKK95_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOC18570.1"
                     /translation="MTSYLRIFTSAGWRLALTTIALAAAAAACGSKPADKPASANAWA
                     VVNAHEITADDVDKALRRSEQGGQPLGEDEMFAAKLALLDDLISQEIFLAKARDLAID
                     VPQPELDTAIAEARKNIPEETFKQELARRNLTEADIREGLHREMLIRKLFEREVSSKV
                     SVTDQEISAFYEANKPQFNRTEDAYRVAQIVVTPVKEEQVNNRTGSDATTALESVQKY
                     RMIMEKLKSGAQFGEVAADYSEDPQSALRGGDLGFIPLSVIQKYPPRLRDAVLKAKPG
                     AAQAIGDGNGFLIVLVLGKDSAGQKDLETPGVKDAIRESLKSRREQLLRAAYIDSLRN
                     GARVENLIARRVVESQGKVPGPAPAAPAAR"
     gene            5353..5712
                     /locus_tag="PKK95_09895"
     CDS             5353..5712
                     /locus_tag="PKK95_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18571.1"
                     /translation="MRIRVLSLLFVLVLTLGTAAHDLDITGKWRGTAATGTEIALDLA
                     TDGSTVTGTVSLNRADPRPISDGRFEDNHLSFTMPSMFGNDTVTVSGTLEENELALVL
                     ESERGTTTARLKREPAS"
     gene            5886..6773
                     /locus_tag="PKK95_09900"
     CDS             5886..6773
                     /locus_tag="PKK95_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOC18572.1"
                     /translation="MTRRDFVAAGLALAGVPPSRLAAADTPAGPVPAPKTGVRVGMAQ
                     ILVKASAADENLARAEAAVARAKALDCDFVVLPECLDLGWTNPAARQLATAIPGPFSD
                     RLAEAARKHAIHVVAGLHERDGERLFNASVLFDDTGRLLHKHHKINELDIAWDLYTRG
                     DSLAVAPTRFGKVAISICADNAPASVSLGHAVGHLGARMLLSPCAWAVPADYDPVKEP
                     YGWNVWDDSYPDIARRHRMPVIGVSNVGAMEAGPWKGRLCIGSSLAVSGHGTVLARGP
                     YGVDAEALVTVDLPLAAAG"
     gene            complement(6807..>7368)
                     /locus_tag="PKK95_09905"
     CDS             complement(6807..>7368)
                     /locus_tag="PKK95_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOC18573.1"
                     /translation="RDDGTPAAGNPLAAKGFHPAVWSYGHRNQLGIAFDESGNLWEQE
                     MGPRGGDEVNLILPGRNYGYPIVSDGDHYDGRPIPDHSTRPEFEKPKVSWNPVISPAG
                     LMVYSGKLWPQWKGDLFIGGLSSRALIRVDIDGTNARKGDQWPMGERIREPEEGPDGA
                     IWLLEDGRQGGSGRLLKLTPTGSGSH"
     assembly_gap    7369..7378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7379..>7962)
                     /locus_tag="PKK95_09910"
     CDS             complement(<7379..>7962)
                     /locus_tag="PKK95_09910"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOC18574.1"
                     /translation="ASLTGAVLVLLAAAWAVNAERRSSGVAGQAGSLPFQVQVMADFE
                     SPWAMTFLPDGRMLITEKSGVLYLVSADAQQRTRVDGIPEVADEGQGGLMDVVLHPAF
                     PQNRFVYFSYSEAGQGGKGVVLARGVLADGERSALQKVETLFRAEPYVDGDGHFAGRI
                     AFSRDGHLFFTNGERQKFVPAQDPKATLGKILRL"
BASE COUNT         1145 a         2829 c         2685 g         1293 t
ORIGIN      
        1 acgtgttcgc cgctctgtcg gggattcccc ttcacctcgt ctcgcgcgca ccgggcagcc
       61 gccacatcgc gttcgtgctt gcggacgccg acctcccgac tgcgacggcc cgcctgcacc
      121 acgccttcct gtcggctccg gtttggcacc acagccccgc cgccctcagg gtcgccgcgg
      181 cgggaggtgc gagatgagtc tttgaggccc catacgtcgg gcggccggcc gaggccgccc
      241 cgaggaggat ggatcgatgt ctcgacacgg atcgttcggc cccgccacga ccgcgctgca
      301 cggcggccac acgccagacc cggccaccgg cgcctgtgcc gttccgatct accagaccgc
      361 gtcctacgcg ttcgagtcgg tggaccaggc cgccgcgctc ttctcgctcg agaagccggg
      421 ctacatctac acccgcatcg gcaaccccac caccgacgtt ctcgagcgga ggcttgccga
      481 gatggaggga ggggtgggcg cgctggcact ggcgtcgggc caggcggcca tcaccgccgc
      541 ggtgctcacg gtcgcgcgcg ccggcgacaa catcgtctcc accgcgtcgc tctacggcgg
      601 cacggtcaac ctcttccacc acacgctcgc gcgcatgggc atcgaggccc gtttcgtgga
      661 cacctccgac ccggcccggg ttgcggcggc catcgacgac aggacccggc tcgtctacac
      721 cgagtcgatc ggcaacccgc gcaacaacgt ggacgatctc gaggccctcg ccggcacggc
      781 ccacgcggcc ggcgtcccct tcgtggtgga caacaccgtg acgcctcacc tgctcaggcc
      841 tttcgaccac ggcgccgacc tcatcgtcta ctcgttgacg aagttcgtcg ggggccacgg
      901 cacctccatc ggcggcgcgc tggtcgactc gggccggttc gactgggcga acggccgatt
      961 ccccgagttc acgagccccg acccgtcgta ccacggcctg gtcttctggg acgccttcgg
     1021 cctccacgac cgtgccgtgc ttcgaggcgc cgcgttcatc accaaggcca gggtgcaggt
     1081 gctgcgggac ctcggcgcct gcctctcgcc cttcaacgcg ttcctgttcc tgcaggggct
     1141 cgagacactt ccctaccgga tggcggcgca cgcatcgaac gcgaggcgcg tggcggagtg
     1201 gttgagcgtc caccccgacg tcgcctgggt gaactacccg ctgctgcccg agcaccccga
     1261 ccacgagcgc gcccggcgct acttccccgc cggcgccggc gccatcgtcg gcttcggcat
     1321 ccgcggcggc cgcgaggcgg ccgagcggtt catctccggc gtccggctgt tcagccacct
     1381 ggcgaacatc ggcgacgcga agagcctcgt cattcacccg gcgtccacca cgcatcagca
     1441 gctgacgccg gagcagcgga aggccgcggg cgtgaccgac gacttcatcc ggctctcggt
     1501 ggggatcgag gacgccggtg acctgatcgc cgatctcgag caggccatcg ccagggcacg
     1561 aaaggtcacg gcggcatgaa cgacgaggga gaaggcagtg tcggccaggt cgagacgagg
     1621 attgtcgagg tgtgcacggg cggtctgacg ctcgagagcg ggcggcgcct cgaatcggtc
     1681 gaggtcgcct acgagcaata cggcgagatc gacgcggagc gcgacaacgt ggtgctcgtg
     1741 tgccacgcgc tgtcaggagg cgcgcacgcg gccggatggc atccgggcgc gcggaagccc
     1801 gggtggtggg acaacctgat cggccccggc aaggcgttcg acacccggaa atactgcgtg
     1861 atctgctcca acgtgctcgg cagctgctac ggcaccaccg gcccctcgtc tgctgagccg
     1921 gcaaccggcc ggccctacgg cagccgcttc ccggtggtga cggtcggcga catggtgacc
     1981 gtgcagaagg ccctcctcga tcggctcggg atccgccggc tgctcgccgt cgttggcgga
     2041 tcgatgggcg ggatgcaggc gctgcagtgg gccgtcgcct gccccgcgtc ggtccgctcg
     2101 gtgatcgcaa tcgcttcctc cgcccggcac tcgccccagc agattgcctt caacgagatc
     2161 gccagacgcg cggtgacggc cgacccggcc tggtcgggcg gcgactacta tgggagcgag
     2221 cggccggacc gcggcctggc ggtcgcccgg atgatcgggc atgtcaccta cctctccgcg
     2281 ggcggcatgg agcggaagtt cggccgcagg gcggcggcgc ccgccccgtc ctggacgctc
     2341 gagccggagt tcgaggtcga gcgctacctc tcgcaccagg gccgggagtt cgcggaccgc
     2401 ttcgacgcca actcgctcgt ctacctcacg cgggccatcg actacttcga tctgggcgcg
     2461 gggtggccgt cgctgaccga ggcgttccgc gcggcgcgcg catccttcct gctgctgacc
     2521 ttctcatccg actggctgta cccgccctct cagctggccg aggtcggccg ggctgccagc
     2581 gacgcgggcc gcgctgtgga gtaccacgag atcccgagcg gctacgggca cgacgcgttc
     2641 ctgctggaga gcgaggcgca gactccgatc atccgctcgt ggctggagcg ggtggccgag
     2701 gcttcccggc agccgcgcgt gtcgatgccg gcactggcga ggtcgaggag ttcttcttgc
     2761 atcccagccg gggcaggagg gcgaggtggt tgagcggcct cccgcgaccg agcgggagaa
     2821 gatgctggcg ggtcagttgt acgacccctt cgaccccgat ctggtggccg gccgcgaacg
     2881 cgcgcgcgat ctctgccagg cgctgaactc ctccggcgag cggcggcagg aggaacggcg
     2941 ccgcatcctg cgcgagctgt tcgccaaggg tggagagtcg gtgtggatgc agcccccgtt
     3001 cttctgcgac tacggggtga acatcgagct gggcgagcgt gtcttcttca acttcaactg
     3061 cgtcgttctg gacgtgtgcc gcgtgcgaat cggcgactgc acgctgtttg ggccggccgt
     3121 gcaaatctac acgccgatgc acccctggaa cgccgggcag cgccgggtga gcgagttcgg
     3181 caagcccgtc gagatcgggt ctgacgtctg ggtggggggc ggcgccatca tcctgcccgg
     3241 agtgaagatc ggctcgagga cggtgatcgg agcgggcagc gtggtcacgc gtgatcttcc
     3301 cggcgacgtg tttgcggcgg ggaatccgtg ccgggtgatt cgggagatcc gcgagtgatg
     3361 ccggccgggc cggcaccggc tggccccggc actggaagcc gggagcgccg cccaagaagc
     3421 cggcgctcga agccgacccg cccgctcacc cgcggcgcgc ccgttcgatc gtggcgacgt
     3481 cgatcttcct catcggcatc atcgcctcga acgcgcgcct cgcttcgtcg ccgccggccg
     3541 cgagcgcctc ggtgaggacc ctcggcgtga tctgccagga cacgccccac ctgtccttgc
     3601 accagccgca ggcgctctcc tggccgccgt tccccacgat cgcgttccag tagcgatcgg
     3661 tctcttcctg gtcctcggtg gcgatctgga acgagaacgc ctcgttgtgc tggaacacgg
     3721 ggccgccgtt caagcccacg caggcgacgc cggcgacgac gaactcgacc gtcagcacgt
     3781 cgcccttctt cccgccggga aagtcggcgg gcgcgcggag aatcgcaccc atcctgctgt
     3841 cgggaaaggt tgcggtgtag aagcgggccg cggcctcggc gtccttgtcg taccagaggc
     3901 aaatcgtgtt cttcgccatc tgctcgtcct ttccgcaccg gggggagcga tgcggacgct
     3961 gggtgcagat tccgtgccgc tggctgatgc gtccctcccc tgcttctacc tggcggcggg
     4021 cgccgccggc gcgggcccgg gaaccttacc ctgcgactcc accacgcgcc tggcgatgag
     4081 gttctcgacc ctggcgccgt tgcgcaggct gtcgatgtag gccgcccgca gcagctgctc
     4141 gcggcgcgac ttgagcgact ccctgatggc gtccttgacg cccggcgtct cgaggtcctt
     4201 ctggcccgcg gaatccttcc cgagaacgag gacgatgagg aacccgttgc cgtcgccgat
     4261 cgcctgagcc gcgccgggct tggccttgag caccgcgtca cgcaaccgcg gcgggtactt
     4321 ctggatgacc gagagcggga tgaacccgag gtcgccgccg cgcagtgcgc tctgcgggtc
     4381 ctccgagtag tcggccgcca cctcgccgaa ctgggcgccg ctcttcagct tctccatgat
     4441 catccggtac ttctgcaccg actcgagggc cgtcgtcgca tcgctcccgg ttcggttgtt
     4501 gacctgctcc tccttcaccg gcgtcacgac gatctgggcg acgcggtacg cgtcctccgt
     4561 ccggttgaac tgcggcttgt tcgcctcgta gaaggccgag atctcctggt cggtcaccga
     4621 gaccttcgac gacacctcgc gctcgaagag cttgcggatg agcatctcgc ggtgcagtcc
     4681 ctccctgatg tcggcctctg tcaggttccg cctcgccagt tcctgcttga aggtctcctc
     4741 cgggatgttc ttccgcgcct cggctatcgc cgtgtcgagc tcgggctgcg gcacgtcgat
     4801 cgcgaggtcc cgggcctttg ccaggaagat ctcctgcgag atcaggtcgt cgagaagggc
     4861 cagcttggcc gcgaacatct cgtcctcgcc gagcggctgg ccgccctgtt cgcttcgccg
     4921 aagggccttg tccacatcgt cggccgtgat ctcgtgagcg ttcaccacgg cccaggcgtt
     4981 ggccgaggcg ggcttgtccg ccggcttcga gccgcaggcc gctgccgcgg cggccagcgc
     5041 gatggtcgtg agtgcgagcc gccatccggc ggacgtgaag atgcgaaggt aactggtcat
     5101 cgttgacatt ccatgagctg gacgctgctt ctacctgctc cccgattgtt tcacctgcca
     5161 gccaaccgtg tctcttcggc gcggccgccg caggcctgcc cgaacctcgc ggtgcccgag
     5221 gattgtagca ccgcggcacg ggcctaccct ggcattggcc ggccgtccgc ttgccgggcc
     5281 gggctgctcg cgtcgccacc gaaccctgaa gacgtcccct tcgtttcttc agaacaggga
     5341 tggagggccc cgatgcgaat ccgcgttctc tcgcttctgt tcgtgttggt cttgaccctg
     5401 gggacggccg cccacgacct tgacatcacc ggcaagtggc gcggcacggc ggcaaccgga
     5461 accgagattg cccttgacct ggcgacggac ggctcgacgg tgacgggcac cgtgagcctg
     5521 aaccgcgccg atccccggcc gatttcggac gggcggttcg aggacaacca cctctccttc
     5581 accatgccct cgatgttcgg gaacgacacg gtgacggtca gcggaacgct ggaggaaaac
     5641 gaactggcgc tggtgctcga gagcgagcgg ggcacgacca ccgcccggct gaagcgcgaa
     5701 cccgcgtcct gacccggcaa atccgcaaac cgaggcggcc tcccatcgcg agcgtgacta
     5761 ccagccacgc gaccccacaa gtacgttgcg cggccggctg gtgacaggtc accattcatg
     5821 gcgttggcga cggtatacct gtcgctgccg gccaccgaag acccccagca tgcggaggaa
     5881 gacacatgac gaggcgagac ttcgtggccg cgggcctggc gttggccggg gttccgccgt
     5941 cgcgcctggc cgcggccgat acgccggctg gccccgtgcc ggcaccgaag accggcgtcc
     6001 gggttggcat ggcccagatc ctggtgaagg ccagcgccgc ggacgagaac ctggccaggg
     6061 ccgaggcggc cgtggccagg gccaaggctc tcgactgcga tttcgtggtg ctgcccgaat
     6121 gcctcgatct cggctggacc aaccccgcgg cccggcagct ggccaccgcg atccccgggc
     6181 ccttcagcga ccggctggcc gaggcggcgc gcaagcacgc catccacgtc gtggcgggcc
     6241 ttcacgagcg tgacggggaa cggctcttca acgcctccgt gctcttcgac gacaccgggc
     6301 ggctcttgca caagcaccac aagatcaacg agctcgacat tgcctgggac ctgtacacgc
     6361 gcggcgactc gctcgccgtc gcgccgaccc gtttcggcaa ggtggccatc tcgatctgcg
     6421 ccgacaacgc ccccgcctcg gtctccctcg gccatgcggt cggccatctt ggggcgcgga
     6481 tgctcctctc cccctgcgcg tgggcggtac ccgctgacta cgacccggtg aaggagccct
     6541 acggctggaa cgtgtgggac gattcgtacc ccgacatcgc gcggcgccac cggatgcccg
     6601 tgatcggggt ctccaacgtc ggggcgatgg aggccggccc ctggaagggc cggttgtgca
     6661 tcgggagttc gctggctgtc tcagggcacg gcacggtgct ggcccggggt ccttacgggg
     6721 tggatgccga ggccctcgtc accgtcgatc tgccactcgc ggctgccggc tgacaccccg
     6781 agcggcggag gagaaacgac gctccctcag tgcgagcccg agccggtggg tgtcagcttc
     6841 agaaggcggc cgctgccccc ctggcgcccg tcctcgagca gccagatggc gccgtccggt
     6901 ccctcctcgg gctcgcggat gcgctccccc atcggccact gatcgccctt cctggcattg
     6961 gtgccgtcga tgtcgacgcg gatcagcgcc cggctggaaa ggccgccgat gaacaggtcg
     7021 cccttccact gcggccagag cttgcccgag tagaccatca gcccggccgg cgagatgacg
     7081 ggattccagg acaccttggg cttctcgaac tcggggcgcg tgctgtggtc ggggatgggc
     7141 cggccgtcgt agtgatcgcc gtctgacacg atcgggtagc cgtagttgcg gccgggcagg
     7201 atcaggttca cctcgtcacc gccccgcggt cccatctcct gttcccacag gttgccgctc
     7261 tcgtcgaagg cgatgccgag ctggttgcga tgaccgtagg accagacggc cggatggaag
     7321 cccttggcgg caagcgggtt ccccgccgcg ggcgtgccgt cgtcgcggnn nnnnnnnngg
     7381 aggcggagga tcttccccag ggtcgccttc ggatcctgag ccggcacgaa tttctggcgc
     7441 tcgccgttcg tgaagaagag gtgcccgtcg cgcgaaaacg cgattcggcc ggcgaagtgg
     7501 ccgtctccat cgacgtaggg ctccgcgcgg aacagggttt cgaccttctg caacgccgac
     7561 cgctccccgt cagcgagaac accgcgggcc aggaccactc ccttcccgcc ctgcccggcc
     7621 tcggaataac tgaagtacac gaaacggttc tgcgggaagg cggggtgcag caccacgtcc
     7681 atcaggccgc cctgtccctc gtctgccacc tccggaatcc cgtccacgcg cgtccgttgc
     7741 tgcgcgtctg cggacacgag gtagagaacg cccgacttct cggtgatcag cattcggccg
     7801 tccggcagga aggtcatcgc ccacggcgat tcgaagtcgg ccatcacctg cacctggaac
     7861 ggcagactcc ctgcctgccc ggccacacct gaagaccggc gctctgcatt gacggcccag
     7921 gcggcggcca gcagcacgag aacggcaccc gtcaggctcg ct
//
LOCUS       DAOPQX010000166         7950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10182_length_7950_cov_4.342115, whole genome shotgun sequence.
ACCESSION   DAOPQX010000166 DAOPQX010000000
VERSION     DAOPQX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10182_length_7950_cov_4.342115"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1213
                     /locus_tag="PKK95_09915"
     CDS             32..1213
                     /locus_tag="PKK95_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC18575.1"
                     /translation="METKSMGFETKMVHAGASPDPYGSAVVPIYQTSTFAFESAQNGA
                     DRFAGRADGYIYTRLGNPTIRALERNVAELEEGADAVATSSGMGAVNTVYMTLLGQGA
                     HLVSTASVYGPSRLLVEKHFSRFGVEATYLDTSDVCQVRAAIRPNTRMVYVESPSNPA
                     MLVSDIRAIADLAHERGALLVVDNTFASPFLQRPLTLGADVSLHSVTKFLNGHADVVG
                     GIVVARDEALCQRLRALMIATGCNMDPHQAFLVSRGIRTLSLRIERAERNAMQVARWL
                     ESHPKVAWVRYVGLESHPQHELVKRQMSGFGSMISFELKGGFEAGRRLMDNVRLALLA
                     VSLGGVETLIEHPASMTHAGVPSADRADAGITEGLVRYSVGIESVDDLIADLAQALER
                     I"
     gene            complement(1250..2056)
                     /locus_tag="PKK95_09920"
     CDS             complement(1250..2056)
                     /locus_tag="PKK95_09920"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HOC18576.1"
                     /translation="MRRCSSLAALLLAAALSPAAAQPPSGHLLVVGGNGTTDDILQKA
                     VATAGGASGTVVIFPQASELAETGRESAEMWTKAGMGRAIVANPADVPAALRAIGEAS
                     FVWFPGGDQNRLVQALEGTPIPEAIRARYRAGALVGGTSAGAAVMSQWMITGEADLKA
                     IAAGKTELKPGLGLWPEVIVDQHFLKRQRGNRLISAVLDRPSLVGVGIDETTAVFVTG
                     HSFEVLGQNSVLVIDARTAQIDPAAAGELATGRGLIVSVLKAGMQYDLSR"
     gene            2079..2702
                     /locus_tag="PKK95_09925"
     CDS             2079..2702
                     /locus_tag="PKK95_09925"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOC18577.1"
                     /translation="MTFREAVQKLERGLREPLPGLAAQARFAPRPRPGWLPQAAAGLR
                     PAAGLLLLFPIAAEAHIVLTVRSSRLPSHAGQVSLPGGAVEEGESIEEAALREAHEEI
                     GLAPGGVRVLGRLTPLHIPVSGFLLHPVVGVAETLPLLHPADAEVERILLLPLAHLLD
                     PSRVRREQRTRDGLFLDIPYLEVEGAQLWGATAMVCAELLSLLQNGE"
     gene            complement(2711..3256)
                     /locus_tag="PKK95_09930"
     CDS             complement(2711..3256)
                     /locus_tag="PKK95_09930"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(3041..3043),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOC18578.1"
                     /translation="MSGLFTLCATLGIVAVSCALASAQPEPELQPGDPAPAFTLPGSD
                     GRTYSLSDFKGKKTVVLAWFPKAFTGGUTAECKSLRESGDEIRKFDVAFFAASVDTPE
                     ENKKFAEHLELDYPLLSDPGKQVARAYGVVGGDRQVAQRWTFFISPEGKILYVERKVS
                     PSTAGPAVAERLKALGVPPAR"
     gene            3361..5814
                     /locus_tag="PKK95_09935"
     CDS             3361..5814
                     /locus_tag="PKK95_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC18579.1"
                     /translation="MVSASDGAPEVPGFVDHLGDRFIVPQPSGSLLELLYFRPDLAAA
                     PFFEPGVKERLARLANFRHSSYTRVRRFQRGEERGGVALVATHVPGRRLAEILQISAR
                     AGLRPQAPAALALARQLMTAVALMHDYGPDIFHGAITPERLILPGDGRIVVCEYVLGG
                     VFEQAVSAWGVAAVWRDWRVPMLADTGLGRYGRRVDLLQIGIVVLTFLLGRPLSPADY
                     PEGLARLLDEGCEAAAQAGWPAPEARAWLSSMLSPERPDAFRTLVEAQKGFAAACAAA
                     ASAGEVSPAALQAFVDQCEGAAWKQPGDLARPSTPTPATLMSAAAMLDAVSAQTDAPA
                     PDPVASLSADVLASAEPPSGEVSSPDTDWFTPKVDYQQYEQPESCEAVPAFILEPEAP
                     ALPDLGSEVPGPEPAEVEEPGAPETAPEEVAGRAAANVAPPEPPPATPVAPVSVDPPV
                     LEAPAASPSPRWHAAEHTAGDPGAPPRATASARSIPSPRPAPVAGKSGLSGRRHPRWR
                     TVAIAASVLLLVALAAVVAPIAWTRVMGGGLPTGQLAVESAPPGAEVMLDGKAVGRSP
                     VALEAAAGQHHVEVRMGGSSRSEWVSVPEDGRLVHKVSLPEASVQGSLEITTDPPGGE
                     VWLDGSKRGKSPARIDGLTPGPHRIAASGPFGQVEDQEVVVVGGAVTPVTVPTVGWLR
                     IRSSYTLEVSERGRVFGQTSGQALMVPAGRHHFDLVNTSLALKVRQFVEVPAGKVVTV
                     PFEAPMGMMNLTSDEPAEVWLDGRLIGQTPLTTLPATLGSHEVVFRHPRLGDVSYTVN
                     VTLAAPVRLTVTFNKKQLP"
     gene            5828..6931
                     /locus_tag="PKK95_09940"
     CDS             5828..6931
                     /locus_tag="PKK95_09940"
                     /inference="COORDINATES: protein motif:HMM:NF022646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HOC18580.1"
                     /translation="MKPAILAGWLLAAGALAAAAQGTAGPAAPLSEAAAARFAALALA
                     CVHQEYPNKIAHVLSSDADVRAPRDLTPAFFGCYDWHSSVHGHWLLARLARTYPEAPF
                     AGPSRAALARSLTPGHVAAEVRYLNGPGRASFERPYGLAWLLQLAAELREWDSAQARE
                     WSATLRPLEEAAVSRISEWLPKLSKPIRIGEHDQTAFSFGLMLDWARAAAHKEFESLL
                     VSRARAFYLGDRGCPLAWEPSGQDFLSPCLAEADLMRRVLPGDRFAAWLGAFLPGLPE
                     RDDPAWLEVAVVTDPSDPKLAHLDGLNLSRAWMLEGIAGALPPADRRLPGLRGAAARH
                     REAGLRAVTGEHYEGGHWLGSFAVYLTTERGLR"
     gene            complement(6978..>7950)
                     /locus_tag="PKK95_09945"
     CDS             complement(6978..>7950)
                     /locus_tag="PKK95_09945"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOC18581.1"
                     /translation="LRPYEEARARRARPGWDTEPPAPSFVGRAIIEDQPLAALRPYID
                     WTFFFAAWQVKGRFPDVLDHPRHGAAARELYEHAQAMLDEIVAGRSIRARAAYGFWPA
                     EAAADADDIVLFEDASRSRVAARFPMLRQQEDHGDERPHRSLADFIAPHGARACDYIG
                     AFAVTAGIGVDELVARYQADRDDYRAIMVKALADRLAEAFAEYLHQRVRREWGYGRDE
                     ALTNEDLIAEKYRGIRPAFGYPACPDHSEKRRLFALLDATPLGLTLTSSCAMLPAASV
                     SGLYFSHPEARYFNVGRIGRDQVESYADRKGMSVEEVERWLGPNLGY"
BASE COUNT         1095 a         2804 c         2880 g         1171 t
ORIGIN      
        1 gccttcaacc ctcagcgata ccggagccgt gatggaaacc aagagcatgg ggttcgagac
       61 gaagatggtg cacgccggcg cctcacccga cccgtacggc agcgccgtcg tgccgatcta
      121 tcagacctcg accttcgcct tcgagagcgc ccagaacggc gccgaccggt tcgcgggacg
      181 cgcggacggc tacatctaca cgcggctggg gaacccgacg atccgggcgc tcgagcgcaa
      241 cgtggccgag ctcgaggagg gcgccgacgc ggtggcgacc agctcgggga tgggggcggt
      301 caacacggtc tacatgacgc tgctcggcca gggggcgcac ctggtgagca cggcgtcggt
      361 atacgggccg tcgcgcctgc tggtggagaa gcacttcagc cgcttcggtg tcgaggccac
      421 ctacctcgac acatccgacg tctgccaggt tcgcgcggcg atccggccga acacgcgaat
      481 ggtctacgtg gagtcgccct cgaaccccgc gatgctggtg agcgacatcc gggcgattgc
      541 ggacctcgcc cacgagcgcg gcgcgctgct cgtggtggac aacaccttcg caagcccgtt
      601 cctgcagcgg ccgctcacgc tcggcgccga cgtctcgctg cactcggtga cgaagttcct
      661 gaacggccac gccgacgtgg tcggcggcat cgtggtggcg cgcgacgagg cgctctgcca
      721 gcggcttcgg gcgctgatga tcgcgaccgg ctgcaacatg gacccgcacc aggccttcct
      781 cgtgtcgcgg gggatcagga cgctgtcgct ccggatcgag cgcgccgagc ggaacgcgat
      841 gcaggtggcg cgctggctcg agtcgcaccc caaggtcgcc tgggtgcgct acgtgggcct
      901 cgagtcgcac ccgcagcacg agctggtcaa gcggcagatg agcgggttcg gctcgatgat
      961 cagcttcgag ctgaaagggg ggttcgaggc ggggcggcgc ctgatggaca acgtccggct
     1021 cgcgctgctc gccgtgtcgc tcggcggggt cgagacgctg atcgagcacc ccgcgtcgat
     1081 gacgcacgcc ggggtgccga gcgccgaccg ggccgatgcg gggattaccg aggggctggt
     1141 gcgctactcg gtcggcatcg agagcgtgga cgacctgatc gccgacctgg cgcaggccct
     1201 cgagcggatc tgagggggcc gggcctggca ggccaggctt gctccagggc taccgggaca
     1261 ggtcgtactg catcccggcc ttcagcaccg acacgatgag gccgcggccg gtggccagct
     1321 ctccggcggc ggccgggtcg atctgcgccg tccgcgcgtc aatcaccagc acgctgttct
     1381 gcccgagcac ctcgaacgag tgccccgtga cgaacacggc cgtcgtctcg tcgatcccga
     1441 cgcccaccag cgacgggcga tcgagcaccg cgctgatgag gcggttgccg cgctggcgct
     1501 tgaggaagtg ctggtcgacg atcacctcgg gccagaggcc gaggcccggc ttcaactcgg
     1561 tcttgccggc ggcaatcgcc ttcaggtcgg cctcgccggt gatcatccac tgcgacatca
     1621 cggcggcgcc ggcgctggtg ccgccgacca gggcgccggc ccgataacgg gcgcggatcg
     1681 cctccgggat gggcgtcccc tcgagcgcct gcaccaggcg attctggtcg ccgccgggga
     1741 accagacgaa gctggcctcg ccgatcgccc gcagcgccgc cggcacgtcg gccgggttgg
     1801 cgacgatggc gcggcccatt ccggccttcg tccacatctc agccgactcg cggcccgtct
     1861 cggcaagctc cgaggcctgc ggaaagatga ccacggtgcc cgaggcgccc ccggcggtgg
     1921 cgaccgcctt ctgcaggatg tcgtcggtgg tgccgttgcc ccccacgacg agcaggtggc
     1981 ccgaaggagg ctgcgcggcg gcgggtgaaa gagcggcggc gagcaggagg gcggccaggc
     2041 tggagcagcg gcgcattgta ggatagtaat cgagagagat gacgtttcgc gaggcggtgc
     2101 agaagctgga acggggactg cgcgagccgc tgcccggcct tgccgcgcag gcgaggttcg
     2161 ctccccgtcc gcgaccgggg tggcttccgc aggccgccgc cggtctccgc ccggccgccg
     2221 gcctgctgct cctgttcccg atcgccgccg aggcccacat cgtgctcacg gtccgctcgt
     2281 cccggcttcc cagtcacgcg ggccaggtgt cgttgcccgg cggggccgtc gaggaggggg
     2341 agtcgatcga ggaggcggcg ctgcgcgagg cgcacgagga gatcggcctg gccccgggag
     2401 gggtgagggt actcggccgg ctgacgccgc tgcacatccc ggtcagcgga ttcctgctgc
     2461 acccggtggt cggcgtggcc gagacgctcc cgttgctgca ccccgcggac gcggaggtgg
     2521 agcgcatcct gctgctgccc ctcgcgcacc tgctcgaccc ctcgcgcgtc cgccgcgagc
     2581 agcggacgcg cgacgggctc ttcctcgaca tcccgtacct cgaggtcgag ggggcacagc
     2641 tgtggggcgc cacggccatg gtgtgcgccg agctgttgtc gctgctgcaa aacggggagt
     2701 agtccccgcg ctacctggcc ggaggaacgc cgagcgcctt cagccgttcc gccacggccg
     2761 ggcccgccgt cgaggggctg accttccgtt ccacgtacag gatctttccc tcgggggaga
     2821 tgaagaacgt ccagcgctgc gcgacctggc gatcgccacc gaccacgccg taggccctgg
     2881 ccacctgctt gccggggtcg ctcagcaagg gatagtcgag ctcgaggtgc tcggcaaact
     2941 tcttgttctc ttccggggtg tccacgctcg ccgcgaagaa cgccacgtcg aacttccgga
     3001 tctcgtcgcc gctctcacgg agcgacttgc actcggccgt tcaacccccg gtgaacgcct
     3061 tggggaacca ggcgagaacc accgtcttct ttcccttgaa gtccgagagg ctgtaggtcc
     3121 ggccgtccga cccgggcagg gtgaaggccg gagccggatc ccccggctgc agctcgggct
     3181 cgggctgggc cgacgccaag gcacacgaaa cggccacgat cccgagcgtc gcacagaggg
     3241 tgaaaaggcc gctcattcga ctcctccagt ccggattgtc gcccaggacc tcattgtatg
     3301 ccagccccgg ccgcccgaga cccaaagctg cgggcggttc gtgccgaaac tatggacagc
     3361 atggtgtccg cgtccgacgg cgcgcccgag gtccctggtt tcgtcgatca cctgggcgat
     3421 cgcttcatcg tgccccaacc gtccggcagc ctgctggaac tgctctactt ccggcccgat
     3481 ctggcggcag cgccgttctt cgagcccggg gtgaaagagc gcctggccag gctggcgaac
     3541 ttccgtcact cgtcctacac gcgcgtccgc cggttccagc ggggcgagga gcggggcggc
     3601 gtcgccctgg tcgcgacgca cgttcccggc cgccggctgg ccgagatcct ccagatcagc
     3661 gcccgcgcgg gtctccgtcc ccaggccccg gccgccctcg cgctcgcgcg gcagctgatg
     3721 accgcggtcg cgctgatgca cgactacggc cccgacatct tccacggcgc gatcactccc
     3781 gagcgcctga tcctgcctgg cgacgggcgc atcgtcgtgt gcgagtacgt gttgggcggg
     3841 gtcttcgagc aggcggtttc cgcctggggg gtggcggcgg tctggcgcga ctggcgggtg
     3901 ccgatgcttg ccgacaccgg cctcggccgc tacggccgcc gcgtggatct cctgcagatc
     3961 ggcatcgtcg tgctgacgtt cctgctcggg cggcccctgt cgccggccga ctacccggag
     4021 gggctcgcgc ggctgctcga cgagggctgc gaggcggccg cgcaagcggg ttggcccgcc
     4081 cccgaggccc gcgcctggct gtcgtccatg ctgtcgcccg agcggcccga cgcgttccgg
     4141 acactcgtcg aggcccagaa ggggtttgcc gcggcctgcg cggcagccgc ctcggccggc
     4201 gaggtgtcgc cggccgcgct gcaagccttc gttgaccagt gcgaaggcgc ggcctggaag
     4261 cagcccggag acctggcgag gccctccacg cccacgcccg cgacgctgat gagcgccgcg
     4321 gcgatgctgg acgccgtttc ggcacagacc gatgcaccgg ccccggatcc cgtcgcgtcg
     4381 ctctcggccg acgtgcttgc gtcagcggag ccgccttccg gggaggtttc cagccccgac
     4441 acggactggt tcaccccgaa ggtcgactac cagcagtacg agcagcccga gtcgtgcgag
     4501 gcggtgccgg ccttcatcct ggagccagag gcgcctgcgc ttcccgacct cgggtctgag
     4561 gtgccgggcc ccgagccggc ggaagtcgaa gagccgggag caccggaaac ggcgcctgag
     4621 gaagtggccg gccgagccgc ggccaacgtc gcgccccccg aaccgccgcc cgccacaccg
     4681 gtggcaccgg tcagcgtcga tccgcccgtc ctggaggcgc ccgcggcaag cccctcgcct
     4741 cgatggcacg cggcggagca caccgcgggc gacccggggg cgccgccgcg agccaccgcg
     4801 tccgcccgca gtattccctc gccgcgaccc gcccccgttg cgggcaagag tggcctgtcg
     4861 ggacgccgtc atccccggtg gcgaacggtg gcgatcgcgg catccgtgct cctgctcgtc
     4921 gccctggccg ccgtggtcgc cccgatcgcg tggacgcggg tcatgggcgg cgggctgccg
     4981 accgggcagc tcgcggtgga atcggccccc ccgggcgccg aggtgatgct cgacgggaag
     5041 gccgttggtc gaagcccggt cgccctcgag gccgcggcgg gccaacacca cgtcgaggtc
     5101 aggatgggag gctcgtcacg ctcggagtgg gtgagcgtcc cggaggacgg gcgcctcgta
     5161 cacaaagtat cgttgcccga ggcatcggtg cagggctctc tcgagatcac caccgacccg
     5221 ccgggtggcg aggtctggct ggatggcagc aagcggggga agtcgcccgc gcgaattgac
     5281 ggcttgaccc ccggtccgca ccgcattgcg gccagtggcc cattcggaca ggtggaagac
     5341 caggaggtgg ttgtcgtcgg cggcgccgtg acgcctgtca cggttccgac cgtcggctgg
     5401 ctgcgaatcc gctcctcgta cactctggag gtgagcgaga gagggcgggt gttcgggcag
     5461 acgagcggcc aggcgctgat ggtgccggcg ggacgccacc acttcgacct cgtcaacacc
     5521 tcgctggccc tgaaggtgcg gcagttcgtg gaggtgccgg ccggaaaggt ggtcaccgtg
     5581 ccgttcgagg cgccgatggg gatgatgaac ctcacctccg acgagcccgc cgaagtctgg
     5641 ctggacggtc gcctgatcgg gcagacgccg ctgaccacgc tgccggcgac gctggggtcg
     5701 cacgaggtgg tcttccggca cccacggctt ggagacgtca gctacacggt gaacgtgacg
     5761 ctggccgccc ctgtccgcct gaccgtgacc ttcaacaaga agcagcttcc gtgagatgct
     5821 gacggggatg aagcccgcca ttctcgcagg ctggctgctg gccgccggcg cgcttgccgc
     5881 ggcagcgcag gggacggccg ggcctgccgc gccgctctcg gaggcggcgg ccgcgcggtt
     5941 cgccgcgctc gcgctggcct gcgtgcacca ggagtacccg aacaagatcg cccacgttct
     6001 cagcagcgac gccgacgtgc gggcgccgcg cgacctgacg cccgccttct tcgggtgcta
     6061 cgactggcac tcgtcggtac acggccactg gctgctggcc cgcctggcgc ggacctaccc
     6121 cgaggcgccc ttcgccggtc cgtctcgcgc cgcgctggcc cgcagcctga cacccggcca
     6181 cgtggcggcc gaggtgcgct acctgaacgg cccgggacgc gcctcgttcg agcggccgta
     6241 cgggctcgcc tggctgctgc agctggctgc cgagttgcgc gagtgggaca gcgcgcaggc
     6301 gcgggagtgg tcagcgacgc tccgcccgct ggaggaagcc gctgtctcgc ggatctctga
     6361 gtggctgcca aagctgtcga agccgattcg gatcggcgag cacgaccaga cggcgttttc
     6421 gttcgggctg atgctcgact gggcgcgcgc ggcggcgcac aaggagttcg agtcgctgct
     6481 cgtttcgcgc gcccgcgcct tctacctggg ggaccgcggc tgtccgctgg cctgggagcc
     6541 ctcgggccag gatttcctgt cgccctgcct tgcggaggcc gacctgatgc gccgagtcct
     6601 gccgggcgac aggttcgcgg cgtggctcgg cgccttcctg ccaggcctcc cagaacggga
     6661 cgatcccgcc tggctcgagg tcgcggtggt caccgacccg tccgatccca agctcgcgca
     6721 cctcgacggt ctcaacctga gtcgcgcgtg gatgctggaa gggattgccg gcgcgcttcc
     6781 gccagcagat cgccggctgc ccgggcttcg cggggccgcg gcccggcatc gcgaggctgg
     6841 ccttcgggcc gtcacgggcg agcactacga gggaggccac tggctcggca gcttcgccgt
     6901 gtacctcacg acggagagag gcctgaggta gaggcgagcc cgacccctga atcccgaatc
     6961 ctgaatcccg aatccggtca gtatcccagg ttcggtccca gccagcgctc gacctcctcg
     7021 acgctcatgc cctttcggtc tgcgtacgac tccacctggt cgcggccgat ccgtcccacg
     7081 ttgaagtacc gcgcctcggg gtggctgaag taaaggccgc tcacactggc ggccggcagc
     7141 atcgcacagg aggaggtcag ggtgaggccg aggggagtcg cgtcgagcag cgcgaagagc
     7201 cggcgtttct cgctgtggtc ggggcaggcg gggtagccga acgcgggccg gatgccgcgg
     7261 tacttctcgg caatcaggtc ctcgttggtc agcgcctcgt cgcgcccgta gccccactcc
     7321 cggcgcacgc gctggtgcag gtactcggcg aacgcctctg cgagccggtc ggcgagggcc
     7381 ttcaccatga tggcgcggta gtcgtcgcgg tcggcctggt agcgggcgac cagttcgtcc
     7441 acgccaattc ctgcggtgac cgcgaaggcc ccgatgtagt cgcacgcccg tgcgccgtgc
     7501 ggggcgatga aatcggcgag cgagcggtgc gggcgctcgt ctccgtggtc ctcctgctgg
     7561 cgcagcatgg ggaagcgcgc ggccacgcgc gacctcgagg cgtcctcgaa gagcacgatg
     7621 tcgtcggcgt cggccgcggc ctcggccggc cagaacccgt acgcggcccg cgcgcggatc
     7681 gatcggccgg ccacgatctc gtcgagcatc gcctgggcgt gttcgtacag ctcgcgggcc
     7741 gccgcgccgt gccgggggtg atcgaggacg tcggggaagc gtcccttcac ctgccacgcg
     7801 gcgaagaaga acgtccagtc gatgtagggc cgcagggcgg ccaggggctg gtcctcgatg
     7861 atcgcgcgcc ccacgaacga cggcgccggt ggctcggtgt cccagccggg gcgggcgcgg
     7921 cgggcgcgcg cctcctcgta agggcgcagc
//
LOCUS       DAOPQX010000167         7925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10224_length_7925_cov_4.418933, whole genome shotgun sequence.
ACCESSION   DAOPQX010000167 DAOPQX010000000
VERSION     DAOPQX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10224_length_7925_cov_4.418933"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PKK95_09950"
     CDS             complement(<1..413)
                     /locus_tag="PKK95_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18582.1"
                     /translation="MIGIFLGYLALTVILGLWQARRTRSQADFVLGSSRLPGWMLALS
                     ERATGESAWLLLGLTGFVYANGLSAIWIAVGCLTGISTAWVVLARRFRSEAERYRALT
                     MPDYFSAKFPEKAYAIRLLSTLIIVFFFVFYVGAQ"
     gene            528..1616
                     /locus_tag="PKK95_09955"
     CDS             528..1616
                     /locus_tag="PKK95_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC18583.1"
                     /translation="MLLLLALLVPGVSIAAVARAQEPTAANVIVVTLDGLRWQEYLGG
                     ADRELLKDVGKPDPPTIVKRFWRETAGARREALMPFMWGVVATRGQVFGDPALGSLAR
                     VANGVWFSYPGYAELLSGFADPRVDSNDKVPNPNVTVLEWLNRRPGFEGRVFAFGAWD
                     VLGSILAVERSGLPAGDGYPPVPDPKTDRERAINDFADDLPPLWEGAPLDAPIMQAAL
                     ECLRVRQPRVLYVMLGETDEWGHEGRYDLYLDAAWRGDRFIRRLWETAQSMPAYAGKT
                     ALVVAVDHGRGATKEDWTDHGRKVPAAERVVMAVMGPDTPALGLRKGVTVTAGQLAAT
                     AAALVGEDFRAAAPKAAPVLPDVLAKTP"
     gene            complement(1629..2771)
                     /locus_tag="PKK95_09960"
     CDS             complement(1629..2771)
                     /locus_tag="PKK95_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOC18584.1"
                     /translation="MAVERGKEIVRQWSILRQIANARDWTVPKLAREHGVCTRTIYRD
                     LAALQAAGFPLYDEEREGRKYWRLESWPFPRLHDVGFTFPELCAFYIHRGRLSGPLPL
                     AEDLSSAMEKVLGTLPPALKDYLDRLAAVVCWKSDVPVPAAGPIDQEALVRAAIEHRR
                     VVMSYRSFSSGRVKEYTVEPYRLSAGNGSLYLYAYVPVYGEMRTFAVQRIQQLRVLDD
                     HFDPTGLPAGEPFGNSIGIGSGKPELVEVWFAPKLTAYIEERTWHPSQRLTRQPDGSL
                     VMALRVAIDAPLRSWILGWGHLAQVLAPSKLATEILEELREASEHYAPRIPFELQPAV
                     NDAAPEAVLPFMAPRGEGARSGRAGRGDGRPQGPPLRRVSRGARQG"
     gene            complement(2932..4077)
                     /locus_tag="PKK95_09965"
     CDS             complement(2932..4077)
                     /locus_tag="PKK95_09965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC18585.1"
                     /translation="MAHGLETIDADQAAIPFSSLKLDRRIELGLADRRYVETTPIQSV
                     TIPAVLEGVDLIGCAETGTGKTAAFLLPIMQRLLAGDRDGQGGTRVLILAPTRELAVQ
                     IEDEFQGLAYHTSLSVAAVYGGVPGGPQERALTAGADVVVATPGRLLDHMSSGSTRFE
                     ALDVLVLDEADRLLDMGFWPDVRRIVSALPSSRQTLFFSATMSEDVFKSAKEIMREPK
                     LVQVGQTTMPDTITHVGAEVPSGEKAGWLARFLRETGGPAIVFVRTKRGADRLASRLA
                     SAGIRCAALHADRTQSERTRAVEGFKSGRYTTLVATDIAARGLDIEGVAHVVNFEVPF
                     SADTYVHRVGRTGRAHATGTAVTLVDAAERATLEALERSLDLSLKRS"
     gene            4370..5281
                     /locus_tag="PKK95_09970"
     CDS             4370..5281
                     /locus_tag="PKK95_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18586.1"
                     /translation="MKLNTLSLALHVGDPIAVSNLTVFPLLAGEESPAGYATLDEALA
                     AGFVRVTEVSDAGHVPELTIVNDGAVPVLVLDGEELVGAKQNRIVNLSIMVPPRSKLP
                     LPVSCVEAGRWAHRSRTFAAAGRAHYASGRAMKLRQVSYFMHASGARHADQGAIWNDI
                     DAKAERMAACSPTHAAAAMYDQAREALETFQSRLQAAPGQVGAIFAINNRIVGLEAFD
                     SAATWRKSMRKIVESYGLDALDRRGEPGSRGRRDPGRFLSSVASGEAERFKAVGMGED
                     LRLRGRGVEGAALAVDDEVVHVLAYAE"
     gene            complement(5302..5889)
                     /locus_tag="PKK95_09975"
     CDS             complement(5302..5889)
                     /locus_tag="PKK95_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOC18587.1"
                     /translation="MTVRATTLIADGNSLTPKQYLEQVARLDPDDYVALLSVLDQPYW
                     QRAEYRVPDNWWHDKSYLNVPLDTFMAAFKDAVRKGYSVVVAADMSEPGYSIGPPGIA
                     IVPSWDVPASHIDENARHFRFATGNTTDDHALHVVGWVQRNGRDWYLVKDSWSSAWNN
                     EHPGYYFFHEDYVKLKVLSFTVHKDALTDVLARFE"
     gene            5935..6399
                     /locus_tag="PKK95_09980"
     CDS             5935..6399
                     /locus_tag="PKK95_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOC18588.1"
                     /translation="MHLLHFGIYGTCFALAVLSSIFPLVNAEVVLLGYVVAAKSDWRE
                     ALLFAAIMAVGQMVGKAGMYWFGRGAARVPSERTRRSIERWEARLRRSPRSVMLFVLL
                     SASTGFPPFYVLSILVGTLRINFWAFLGVGLVGRLLRFGLIAMFPSGISRLR"
     gene            complement(6440..7309)
                     /locus_tag="PKK95_09985"
     CDS             complement(6440..7309)
                     /locus_tag="PKK95_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HOC18589.1"
                     /translation="MRIAALVLLFGCLGCAHATVPAPAGPASTVLPASHPECQPATAL
                     VNAVLWMQTAAEYRASARQVYTAARQALDAALADPEWVGATEERQGEAGQPPAIILDL
                     DETVFDNSPFEARMIRRGIRFNQAAWDEWVEEAAAAAILGAADFLLYARGRGVTPFYV
                     TNRAAGEEAATRRNLQKLGLPLSTEPDTVLVLGERPEWKTGDKSPRREWVAQSYRVLL
                     LVGDDLNDFANAGDLDLAARNEIVEEHAAWWGRRWFVVPNPAYGSFERAVTGPDGTPC
                     EQLQRKVNALRER"
     gene            complement(7369..>7925)
                     /locus_tag="PKK95_09990"
     CDS             complement(7369..>7925)
                     /locus_tag="PKK95_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HOC18590.1"
                     /translation="ARRTAWAAGLAGLALLAGSGVGFARTVSLYVERHRSMPTLFALD
                     GSWWERELIEEEGSRLTPSAGLARLDLAPGKYPGLDFEEPYPDWRGYRTLALTIVSDL
                     DAPLTMTVRIHDARHNRRLEDRFNRRLVVQPGENTFRIPIDEIRRAPRGREMDLARIR
                     GIMIFSGRLDHPTHVYLGPLRLEQ"
BASE COUNT         1135 a         2685 c         2905 g         1200 t
ORIGIN      
        1 aactgggcgc ccacgtagaa cacgaagaag aagacgatga tgagggtgga gagcagccgg
       61 atggcgtacg ccttctccgg gaacttcgcg gagaagtagt cgggcatcgt cagcgcccgg
      121 tagcgctccg cctcggaacg gaagcgccgg gcgaggacga cccaggcggt cgagatgccg
      181 gtgaggcagc cgaccgcaat ccagatcgcg gagaggccgt tggcgtagac gaacccggtc
      241 aggccgagca gcagccaggc cgactcgccg gtcgcccgct ccgagagggc cagcatccat
      301 cccggcagcc gcgaactgcc gaggacgaag tcggcctgcg accgcgtccg ccgcgcctgc
      361 cagaggccca ggatcactgt cagggccagg tagccgagga agatgccgat catcgcacgc
      421 tcctgccagg ggcgacgggg cattctctca cagggatcag ggatcgggcg tgtagaatgc
      481 agagccagct ggagacttgt atgaagtgtg tgtgctgcag gttccgcgtg ttgctcctgc
      541 ttgccctcct ggtgccggga gtgtccattg cggcggtggc gcgggcccag gagcctacgg
      601 ccgccaacgt gatcgtcgtt acccttgacg gcctgcggtg gcaggagtac ttggggggcg
      661 ccgaccggga gctgctgaag gatgtgggga aacccgaccc gccgaccatc gtcaagcggt
      721 tctggcgcga gacggccggg gcaaggcgcg aggcgctgat gcccttcatg tggggtgtcg
      781 tggccacccg ggggcaggtg ttcggcgacc cggcgcttgg gagcctcgcc cgggtcgcca
      841 acggggtctg gttctcgtac cccggctacg cggagctgct ctcgggtttc gccgatcccc
      901 gcgtggacag caacgacaag gtgcccaacc cgaacgtcac cgtcctcgag tggttgaacc
      961 gccgcccggg cttcgagggc cgcgttttcg ctttcggcgc gtgggacgtg ctcggatcga
     1021 tcctggccgt tgagcgcagc ggcctgccgg ctggcgacgg ctacccgccg gtgcctgacc
     1081 cgaagaccga ccgcgaacgg gccatcaacg acttcgccga cgacctcccg ccgctgtggg
     1141 agggggctcc gctcgacgcg cccatcatgc aggcggcgct cgagtgcctg cgcgtgcggc
     1201 agccgcgcgt gctctacgtg atgctgggcg agaccgacga gtggggccac gaggggcgct
     1261 acgacctgta cctcgatgcg gcgtggcgcg gcgaccggtt cattcgaagg ctctgggaga
     1321 cggctcagtc gatgccggcc tacgcgggga agaccgccct ggtggtggcg gtggatcacg
     1381 gccgtggcgc gacgaaggag gactggaccg accacggccg gaaggtgccg gccgccgagc
     1441 gggtcgtgat ggcggtgatg gggcccgaca ccccggcgct tgggctgcgc aagggcgtca
     1501 cggtgacagc ggggcagctc gcggcaaccg ccgcggcgct ggtgggcgag gacttccgcg
     1561 ccgccgcccc gaaggccgcc cctgtgctgc ccgacgtcct ggccaagacg ccgtaggggg
     1621 gccgcccctc acccttgcct ggcgccccgg gagactcggc gtaggggcgg cccttgtggc
     1681 cgcccgtcac cccggccggc cctgcccgat cgtgcaccct ccccgcgcgg cgccatgaac
     1741 ggcagcaccg cctcgggcgc cgcgtcgttg accgccggct gcagctcgaa cgggatgcgg
     1801 ggcgcgtagt gctcgctggc ctcccgcagc tcctcgagga tctcggtggc cagcttcgag
     1861 ggggccagca cctgcgcgag gtgcccccag ccgaggatcc acgaccggag cggcgcgtcg
     1921 atcgccacgc gcagggccat caccagagag ccgtcgggct ggcgggtgag gcgctgcgac
     1981 gggtgccagg tccgctcttc gatgtaggcc gtcagcttcg gcgcaaacca gacttccacc
     2041 aactccggtt ttccgctgcc aatcccgatc gagttgccga acggctcgcc cgcgggcagg
     2101 ccggtggggt cgaagtggtc gtcgagcacg cgcagctgct ggattcgctg gacggcgaac
     2161 gtccgcatct cgccataaac cggcacgtac gcgtagagat agagggagcc gtttccggcg
     2221 ctcagccggt acggttcgac cgtgtactcc ttcacgcgcc cgctcgaaaa cgagcggtag
     2281 gacatgacca cccggcgatg ctcgatcgcg gccctgacca gggcctcctg gtcgatgggc
     2341 ccggccgcgg gcacggggac gtcgctcttc cagcagacga cggccgccag cctgtcgagg
     2401 tagtccttga gcgccggcgg cagcgtcccg agcacctttt ccatcgcgga ggaaaggtcc
     2461 tcggccagtg gcaaggggcc ggacaggcgc ccgcggtgga tgtagaaggc gcatagctcc
     2521 gggaacgtga agccgacgtc gtgcagccgg gggaacggcc agctctcgag ccgccagtac
     2581 ttcctgccct cgcgctcctc gtcgtagagg gggaagccgg cggcctggag cgcggccagg
     2641 tcacggtaga tggtgcgcgt gcagacgccg tgctcgcggg ccagcttggg cacggtccag
     2701 tcccgggcgt tcgcgatctg ccgcaggatg ctccactggc ggacgatctc ttttccgcgc
     2761 tcgacggcca tgtccacctc ccggccgcat taccttacca ccgccgggac tctccacgtg
     2821 tgttgagcga gggctggacg ttcgcaggtc cggcggacgt gccggtctcg gggagggagg
     2881 tgttggggcc gcgcgtgcga gcgaacgcgc ggcttccgtg agtgatgcgg gtcagcttct
     2941 tttcaggctg agatccagcg accgctcgag cgcctcgagc gtcgcccgct cggcggcgtc
     3001 caccagcgtg acggcggtgc cggtggcgtg ggcgcggccg gttcgcccga cgcggtggac
     3061 gtaggtgtcg gcgctgaagg gaacctcgaa gttcacgacg tgcgccaccc cctcgatgtc
     3121 gaggccccgg gccgcgatgt cggtggccac cagcgtcgtg tagcgccccg acttgaaccc
     3181 ttcgaccgcg cgcgtccgct ccgactgcgt ccggtcggcg tgcagcgccg cgcagcggat
     3241 gccggcgctt gccagccgcg acgccaggcg gtcggcaccc ctcttcgtcc tcacgaagac
     3301 gatcgccggc ccccccgtct cgcggaggaa gcgcgccagc cagccagcct tctcgccgga
     3361 cggcacctcg gcccccacgt gggtgatcgt gtccggcatc gtcgtctggc caacctgcac
     3421 gagcttcggc tcgcgcatga tctccttcgc cgacttgaac acgtcttccg acatggtggc
     3481 cgagaagaac agggtctggc gcgaggaggg gagcgccgac acgatccgcc ggacgtcggg
     3541 ccagaagccc atgtcgagca gccggtcggc ctcgtccagc accagcacgt cgagcgcctc
     3601 gaacctcgtg ctgccgctcg acatgtggtc gagcaggcgg ccgggtgtcg caacgacgac
     3661 atcggcgccc gcggtgaggg cacgctcttg cgggccgccg gggacgccgc cgtagacggc
     3721 ggccacgctc agactcgtgt ggtaggcgag tccctggaac tcgtcctcga tctgaacggc
     3781 cagttcgcgc gtgggcgcga ggatgagtac gcgggtgccc ccctggccgt cccggtcacc
     3841 ggccaggagg cgctgcatga tgggcagcag gaacgccgcg gtcttgccgg tgccggtctc
     3901 ggcgcagcca atcaggtcga cgccctcgag gacggccggg atggtcacgc tctgaatggg
     3961 cgtcgtctcg acgtagcggc ggtcggcgag gcccagctcg atgcgccggt ccagcttcag
     4021 cgaagagaag gggatggctg cctggtcggc gtcgatcgtc tcgagcccgt gcgccaccgg
     4081 gcgcgggttc gacgcgacga acgtcggacg gcgagggttc gatgacgggc gcggcgaacg
     4141 gcggcgacgg ggacgcgccg cggggtgggt ggaggcggtt gtagtggatt ccaaacgttc
     4201 aggccctacc gaggcgggag tcaacggccg ggaattcggc ggtgagatac ctcgtaagct
     4261 ccgtcgatta tggcacacaa gggcggaatc gacgaaaaac cggctcatcc gcagaatggg
     4321 ggcgggcgcc gggacccgcc ctgtcacggc ggggtgcgac agtgtcgcca tgaagctgaa
     4381 cacactctcg ctcgccctcc acgtcggaga ccccatcgcg gtctcgaatc tcaccgtgtt
     4441 cccgctcctg gcgggagaag agtcgccggc cggctacgcc acgctcgacg aggcgctcgc
     4501 agccggcttc gtccgcgtga ccgaggtgtc ggatgccggc cacgtccccg agctgaccat
     4561 cgtcaacgac ggcgccgtgc cggtgctggt gttggacggc gaggaactgg tcggcgccaa
     4621 gcagaaccgg atcgtgaacc tgagcatcat ggtgccgccg cgatcgaagc tgccgctgcc
     4681 ggtgtcgtgc gtcgaggcgg ggcgctgggc gcaccgctcg cggaccttcg ccgccgccgg
     4741 ccgggcgcac tacgcgtcgg ggcgggcgat gaagctccgg caggtgagct acttcatgca
     4801 cgcctcgggg gcgcgccacg ccgaccaggg ggcgatctgg aacgacatcg acgccaaggc
     4861 ggaacggatg gccgcctgct cgccgaccca cgccgcggcg gcgatgtacg accaggcccg
     4921 cgaggcgctg gagacgttcc agtcgcgcct ccaggccgcg cccggacaag tgggcgccat
     4981 cttcgccatc aacaaccgca tcgtcggcct cgaggcgttc gacagcgccg ccacttggcg
     5041 gaagtcgatg cggaagatcg tcgagagcta cggcctcgac gccctcgacc ggcggggcga
     5101 gccgggctcg cgcggccgcc gcgatcccgg gcgcttcctg tcgtcggtgg cctcgggcga
     5161 ggcagaacgg ttcaaggcgg tgggaatggg cgaagacctc cgcctccgag ggcggggcgt
     5221 cgagggcgcc gcccttgccg tggatgacga ggtggtgcac gtgctggcct acgcagagta
     5281 gggcaggcgc agtgcctgcc cctactcgaa gcgcgcgagc acgtccgtca gcgcgtcctt
     5341 gtgcaccgtg aagctgagca ccttgagctt cacgtagtcc tcgtggaaga agtagtagcc
     5401 cgggtgttcg ttgttccagg cgctgctcca cgagtccttc acgaggtacc agtccctgcc
     5461 gttccgctgc acccagccga ccacgtggag ggcgtggtcg tcggtcgtgt ttcccgttgc
     5521 gaaccggaag tgccgggcgt tctcgtcgat gtgcgaggcc gggacgtccc agctcggcac
     5581 gatggcgatt ccaggcgggc caatcgagta gccgggctcc gacatgtcgg ccgcgacgac
     5641 aaccgagtag cccttgcgga cggcgtcctt gaaggcggcc atgaaggtgt cgaggggcac
     5701 gttcaggtag ctcttgtcgt gccaccagtt gtcggggacc cggtactcgg cgcgctgcca
     5761 gtacggctgg tcgagcaccg agaggagcgc gacgtagtcg tccgggtcga ggcgcgcgac
     5821 ctgctcgaga tactgcttcg gggtgaggct gttgccgtcc gcgatcaacg ttgtggctcg
     5881 aaccgtcacc tccggggctt cgtggtacag tgcgagcgcg gactcttctg gcccatgcac
     5941 ctgctccact tcgggatcta cgggacctgc ttcgccctcg ccgtcctcag ctccatcttt
     6001 ccgttggtca acgcggaggt cgtgctcctc ggctacgtgg tggccgccaa gtcggattgg
     6061 cgggaggcgc tgctgttcgc ggcgatcatg gccgtcgggc agatggtggg gaaggcgggg
     6121 atgtactggt tcggccgggg ggcggcgcgc gtcccgtccg agcgaacccg ccggtcgatc
     6181 gagcggtggg aggcccgcct ccggcgctcg ccgcggtcgg tcatgctgtt cgtgctgctg
     6241 agcgcctcga cggggttccc gcccttctac gtcctcagca tcctggtcgg cacgctccgg
     6301 atcaacttct gggcgttcct cggcgtcggg ctcgtggggc gcctgcttcg cttcggcctc
     6361 atcgcgatgt tcccctccgg gatctcccgg ctccgctgac gggatcggcc gccttccgcg
     6421 ggcccgcgcg cggcacggct caccgctcgc gcagcgcgtt caccttccgc tggagctgct
     6481 cgcagggagt accgtcgggg ccggtcaccg cccgctcgaa cgatccgtag gcggggttcg
     6541 ggacgacgaa ccagcggcgg ccccaccagg ccgcgtgctc ctccacgatc tcattccgcg
     6601 ccgccaggtc gaggtcgccc gcgttggcga agtcgttgag gtcgtcgccg acgaggagga
     6661 gcacgcgata ggactgcgca acccactccc ggcgcggcga cttgtcgccg gtcttccact
     6721 cgggacgctc gccgagcacc aggacggtgt cgggctcggt gctcagcggc agccccagct
     6781 tctggagatt gcggcgagtg gcggcttcct ccccggcggc gcggttggtg acgtagaacg
     6841 gcgtgacgcc gcgcccgcgc gcgtacagca ggaagtcggc cgcgccgagg atcgcggcgg
     6901 ccgcggcctc ctcgacccac tcgtcccacg cggcctggtt gaagcggatc ccccgcctga
     6961 tcatccgcgc ctcgaagggc gagttgtcga aaacggtctc gtcgagatca aggatgatgg
     7021 cgggcggctg cccggcctcc ccctggcgct cctcggtcgc ccccacccac tcggggtcgg
     7081 cgagcgccgc gtcgagcgcc tggcgcgcgg ccgtgtagac ctggcgggcg gaggcccggt
     7141 actcggccgc cgtctgcatc cacagcacgg cgttgaccag ggctgtggcc ggctggcact
     7201 cggggtggct cgccggcagc acggtggaag ccgggccggc cggcgccggc accgtggcgt
     7261 gcgcgcagcc caggcagccg aacagcagca cgagagccgc gattctcatg gcaacagcgt
     7321 agcgtaccag cgggaacgcc tgtcacgctg cgatcggatc cgtccgcgct actgttcgag
     7381 gcgcagcggg ccgaggtaga cgtgggtggg gtggtccaag cggcccgaga agatcatgat
     7441 gccgcggatg cgcgcgaggt ccatctcccg cccgcggggg gcgcggcgta tctcgtcgat
     7501 ggggatgcgg aaggtgttct cgccgggctg aacgaccagg cggcggttga accgatcctc
     7561 caggcgccgg ttgtgcctgg cgtcgtggat ccggaccgtc atcgtgaggg gggcgtcgag
     7621 gtcggagacg atggtcagcg ccagggtccg gtagcctcgc cagtccgggt acggctcttc
     7681 gaagtccagc cccgggtact tgccgggcgc caggtcgagg cgcgcgaggc cggccgatgg
     7741 cgtgagccgg ctgccttcct cctcgatcaa ctcgcgctcc caccacgagc cgtcgagggc
     7801 gaagagcgtg ggcatcgacc ggtggcgctc gacgtagagc gagaccgtcc gcgcgaatcc
     7861 cacccccgag cccgccagta gcgcgaggcc cgccagtccc gccgcccagg cggtgcgcct
     7921 ggcgg
//
LOCUS       DAOPQX010000168         7816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10414_length_7816_cov_5.235794, whole genome shotgun sequence.
ACCESSION   DAOPQX010000168 DAOPQX010000000
VERSION     DAOPQX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10414_length_7816_cov_5.235794"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKK95_09995"
     CDS             complement(<1..205)
                     /locus_tag="PKK95_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18591.1"
                     /translation="MSQSKARRSIVRASLAAVLAGWAGLAASALQQPQPGPKASAPPL
                     VYSAIVDALIHPVSAEFMVEAMDR"
     gene            complement(451..2058)
                     /locus_tag="PKK95_10000"
     CDS             complement(451..2058)
                     /locus_tag="PKK95_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18592.1"
                     /translation="MTKPIAAALVLFALAPLAGAQPVRPGEPVLVVPFENASRDASLQ
                     WLAEGSALLLTRDLGRFGASAIPREDRLKAFERLGLPPSALLSDATTIRVGQLIGAAH
                     AAVGTFHAEGGRLELRVRTIRLDTGSAAEEIVERGPVDRLFALVEALARRMVAGTAGP
                     PPVPAGERPLHAFELFVKGLAAETPGAQVRLLQSALALAPGDEEILMALWQAHTTQGE
                     HERALQAVAKVPPGARLARRARFLAALSELSLQRYDAAFEALDGLVAEAPAATAFNNL
                     GVVQLRRAGTRQVARATYYFTKAADLAPEDPDYAFNLGYAYWFERDPQGAIYWLREAV
                     RRNPADPDAHFVLGTALKSAGATIEGERERELARQLSSTYAELHRKRPAGEEVPRGLE
                     RVSLDLEAPRLSLVERTAAPGEQRELSDLAAFHRDRGRRLFDAQRDVEAISELKRSLY
                     LQPYQADVHLLVGRIHQRAGRLHEATESFRISIWCQATPDAHVALGEVLLSLDDALGA
                     QTEANRALALDLSHAAARALLEKATTK"
     gene            complement(2073..2642)
                     /locus_tag="PKK95_10005"
     CDS             complement(2073..2642)
                     /locus_tag="PKK95_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18593.1"
                     /translation="MTEAGIGRVLVASLHQAIADLLPGRLEFYENWLNPTGLRNGTIG
                     LAPLHAVLSFLRQDDHVYPRVVELAGRYAAEWTMAAQGGRRQWLVRFSPRPFRQRAAI
                     ALIRRTVRQSYAGSRAVVRLRRGRGTVDLRASLFCNVREAAPAPLCGFYAALVSRLLE
                     LHRVDGRVEVRACRAVGDRTCVLDVHPGV"
     gene            2666..3268
                     /locus_tag="PKK95_10010"
     CDS             2666..3268
                     /locus_tag="PKK95_10010"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOC18594.1"
                     /translation="MDAAILAGGQARRLAGRDKSALLVGGLTILQRQLSVLHGVAERV
                     VLIGGRAPRDPAPGLAIEAVPDLVAGGGALGGLHTALSAARSPHVLVLACDLPFVSRA
                     FLRYLAALTGPAYDAIVPRAPDGWQPLCAIYRRDLAAAFGERVASGRLKVVDALAELR
                     VREVGPAEIASVEPDERLFLNVNTPEDLARAIELLHKKSR"
     gene            3265..5277
                     /locus_tag="PKK95_10015"
     CDS             3265..5277
                     /locus_tag="PKK95_10015"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002447.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOC18595.1"
                     /translation="MILPVHDALRRRITAALQALYGLDAEEVPAFPINYPPSRELGDL
                     AIPVAFELARRLRRPPKAIAEELAGSIGGVPGVARVEATRGYLNVFLDRGTFLRGRLE
                     PSSPEPPPAAGPKTIVEHTAINPNKAAHIGHLRNAALGDTLVRALRFRGERVEVQNYI
                     DDTGVQVADVVVGFRELEGKGLEEVRSVAGQPRFDYYCWDLYARVSEWYEEDRARLEA
                     RGRALHDIEHGGNESAEIAALVADRIVRCHLQTMARMNVDYHLLAWEGDILRLQFWAR
                     AFEVLKQAGAVYLQSQGRLAGCWVMAIDDGDAPEAVAEAGGLDEEREGEREKVIVRSN
                     GTVTYVGKDMAYQFWKFGLLGRDFHYRPFERDADGEVIWATSSVEVAGGEAHPPFGGA
                     AAVYNVIDVRQSYLQKLLKQALVAIGHPREAERSSHFSYEMVALTHNTARQLGYELSA
                     EDAARPFVEVSGRRGLGVKADDLLDVLVAKAGEEVGRRNPDLDEAAAGKIARAIAIAA
                     VRYFMIRFSRGKVIAFDIDEALNFEGESGPYLQYAVVRANNIFQKLRDREGIGEAEFL
                     SPLGGTPEQELAGEVHGHDLWALVLEAARLDEVVEQAVRTLEFSVLAKYAFGLAQMFN
                     AFYHRYPILNEERDDARRWRAAAVSYCRNQLTAALGLMGIPVPPRM"
     gene            5308..5994
                     /locus_tag="PKK95_10020"
     CDS             5308..5994
                     /locus_tag="PKK95_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOC18596.1"
                     /translation="MSPIVGITPCRSLPDYLESVRRAGGEPRVLDPLKDRPEAVISEL
                     GGLLLTGGVDVDPARYGEERQPTVTCVEPDRDAFEFGLFDAARAANLPVFGICRGIQV
                     MNVAMGGTLVQDIPTQMAGSLEHTIPAPAFAVAHEIWVSKGSRLWTLMQEKMADGDGC
                     SVNSRHHQAVKKVAPGFEVTATAPDGVVEAIECAHSPFCLGVQWHPENFWRTGEFRPL
                     FEGFIEACRG"
     gene            6051..7046
                     /locus_tag="PKK95_10025"
     CDS             6051..7046
                     /locus_tag="PKK95_10025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC18597.1"
                     /translation="METRARTVSLVRRGVTNLLAKRPFCISFEVTYNCNAQCGHCHLG
                     GAANNPREVRAPAERFGELARRFKPVVAQVSGGEPLLRADIDDIVSAIRVPNRAPFVV
                     LTTNGFLLTRERYERLRKAGVDEFSLSFDFPDERHDHFRGIPGLFRRIASLMEGFRPD
                     EAKAITLSAVVHRDNFRDLEALAEQARAWGVRMNFSTYTWLRTGKKELMIPASEMDEF
                     KAVIERLIAHKRRHQTIYTSDYVFRNMIRFFEHGAVPGCQAGVKFFVVNPAGSVSPCG
                     LIIKNYESPAQIREEFLAQNDCTACYTSIRGGSERPLRYLIGDNLNRLRLPATSM"
     gene            7043..7714
                     /locus_tag="PKK95_10030"
     CDS             7043..7714
                     /locus_tag="PKK95_10030"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOC18598.1"
                     /translation="MNEAVEPQVDRARRRRTRILLAAIPAAFLVGLVGLWIAAPDIRQ
                     SKSLWVLFLYCFPSEFVIAVVPHEPALLYFAKFHDPWTIAGVSAVGTVLTEWVNYLVV
                     AWIADVSFFRKLIRGKAVTRLIGWFHRAPFATLLVAGITPVPFYPFRFLAVIGRYPLP
                     KYLLAVLMSRTPRFWIICVAGAALGFSDAVIVAFALVLAAAGLAPAARALWRKKLRPA
                     ALKND"
BASE COUNT         1141 a         2710 c         2813 g         1152 t
ORIGIN      
        1 cgcgatccat cgcctcgacc atgaactcgg ccgagacggg gtggatgagc gcgtccacga
       61 tggccgagta gaccagcggc ggggcggacg ccttcgggcc cggctgcggc tgctgcagcg
      121 ccgacgcggc caggccggcc cagcccgcca ggaccgcggc cagcgaagcc cgcacgatgg
      181 agcggcgcgc tttggactgg gacaagggga tcctcgagcc ctcgcaacgg atgggcgcac
      241 gttgattata cctactcctg ccaatcggca aggccgtgcg acagaccact gtcacgagaa
      301 caaccacaaa ggacacgaag gacacgaaga acaccgcgag acggtcagcg ccggccttcg
      361 gcggccgccg cctcgtccgc gcagggccct gacccggcct tcctttgtga tctttgtcgt
      421 tcaagccgtc cgtggacagg tcgccgcgac ctacttggtg gtcgcctttt cgagcagagc
      481 gcgcgcggcg gcgtgcgaca ggtcgagcgc gagcgcgcgg ttggcctcgg tctgtgctcc
      541 cagcgcgtcg tcgagcgaga gcagcacttc gccgagtgcg acgtgcgcgt cgggggtggc
      601 ttgacaccag atcgagatgc ggaacgactc ggtggcctcg tgcaggcggc cggcgcgctg
      661 gtggatgcgg cccacgagca ggtgcacgtc ggcctggtag ggctgcaggt agagcgagcg
      721 tttcagctcg gagatcgcct cgacgtcgcg ctgcgcgtcg aacagccggc gcccgcgatc
      781 gcggtggaag gccgcgaggt cgctgagctc gcgctgctcg cccggcgcag ccgtgcgctc
      841 gaccagcgac aggcgcgggg cctcgaggtc caggctcacc cgctcgaggc cacgcggcac
      901 ttcctcgcct gccgggcgct tgcggtgcag ctcggcgtag gtcgacgaca gctggcgcgc
      961 cagctcgcgc tcgcgctccc cttcgatggt ggccccggcc gacttcagcg cggtgccgag
     1021 gacgaagtgg gcatcggggt cggcggggtt ccgtcgcacg gcttcgcgca gccagtagat
     1081 ggccccctgc ggatcgcgct cgaaccagta ggcgtagccg aggttgaagg cgtagtccgg
     1141 gtcctcgggc gcgaggtcgg ccgccttggt gaagtagtag gtcgcgcgcg ccacctgccg
     1201 cgtgccggcg cgccgcagct gcacgacgcc caggttgttg aaggcggtcg cggcgggtgc
     1261 ctcggcgacg agcccgtcca gcgcctcgaa cgcggcgtcg taacgctgga gcgacagctc
     1321 cgacaacgcc gccaggaagc gcgcccggcg cgccaggcgg gcgccgggcg gcaccttggc
     1381 aaccgcctgc agggcccgct cgtgctcccc ctgtgtcgtg tgggcctgcc acagcgccat
     1441 gaggatctcc tcgtcgcccg gtgcgagcgc cagcgccgac tgcagcagcc gcacctgggc
     1501 gccgggcgtc tcggccgcga ggcccttcac gaacagctcg aacgcgtgga gcggccgctc
     1561 ccccgctggc acggggggcg gccccgccgt gccggccacc atccgcctgg ccagcgcctc
     1621 gaccagggcg aagaggcgat cgaccggccc acgctcgacg atctcctcgg ccgcgctccc
     1681 cgtgtcgagc ctgatcgtcc ggacccgcaa ctcgagccgg ccgccctcgg cgtggaacgt
     1741 gcccaccgcg gcgtgcgcgg ctccaatcag ctgcccgacg cggatggtcg tggcgtcgct
     1801 caggagggcc gagggcggca gcccgaggcg ctcgaacgcc ttcagccggt cctcgcgcgg
     1861 gatggcggag gccccgaagc ggcccaggtc gcgtgtcagc aggagtgccg agccctctgc
     1921 cagccactgc agggacgcgt cgcgcgacgc gttctcgaag ggaacgacga ggacgggctc
     1981 gcccgggcgg accggctgcg cgccggcgag cggcgcgagc gcgaacaaca cgagggcggc
     2041 cgcgatcggc ttggtcatac acctgggtgg ggtcataccc ctgggtggac gtcgagcacg
     2101 cacgttcggt cccccacggc ccggcaggcc cggacctcca cgcggccgtc gacccggtgc
     2161 agctccagca gccgcgacac cagcgcggca tagaacccgc acaggggtgc cggcgccgcc
     2221 tcccggacgt tgcagaaaag cgatgcgcga agatcgacgg tgccgcggcc ccgccgcagc
     2281 cggacgacgg cccggctgcc tgcgtacgac tgccggacgg tccggcggat cagcgcgatc
     2341 gcggcccgct gccggaacgg ccggggcgag aagcgaacga gccactgccg cctgccgccc
     2401 tgcgccgcca tggtccactc ggccgcgtag cgcccggcga gctccaccac gcgggggtag
     2461 acgtggtcgt cctggcgcag gaagctgagc accgcgtgga gcggggcgag accgatggtg
     2521 ccgttgcgca gtcccgtggg attgagccag ttctcgtaga attcgagccg ccccggtagg
     2581 aggtcggcga tggcctggtg gaggctggcg accagcaccc ggccgattcc cgcttcggtc
     2641 attttctgtt aaggtagaaa cgcccgtgga tgcggcaatc cttgccggag gccaggcgcg
     2701 ccggctcgcc ggccgcgaca agagcgcgtt gctcgtcggc ggcctcacga tactccaacg
     2761 gcagctctcg gtgctgcacg gggttgccga acgggtggtg ctgatcgggg gccgcgcccc
     2821 ccgtgacccg gcgcccggcc tcgcgatcga ggccgtgccc gacctggtcg ccggaggcgg
     2881 cgcccttggc ggcctgcaca ctgcgctgtc ggccgcccgc agcccgcacg tcctcgtgct
     2941 cgcctgcgat ctcccgttcg tcagccgtgc cttcctgcgg tacctcgccg cgctgaccgg
     3001 cccggcgtac gacgcgatcg ttccccgcgc gccggacggc tggcagcccc tgtgcgcgat
     3061 ctaccgccgc gacctcgcgg cggccttcgg cgagcgggtc gcctcgggac ggctgaaggt
     3121 cgtcgacgcg ctggcggaac tgcgcgtgcg ggaagtcggg cccgccgaga tcgcgtcggt
     3181 ggagccggac gagcgcctgt tcctgaacgt caacacgccc gaagacctcg cgcgcgcgat
     3241 cgaattattg cacaagaaga gccgatgatc cttcctgtcc acgacgccct ccgccgccgg
     3301 atcaccgccg cgctccaggc cctgtacggc ctggacgcgg aggaggtccc tgcatttccc
     3361 atcaactacc cgccgtcacg cgagctgggg gatctcgcca tcccggtggc cttcgagctg
     3421 gcgcgccggc tgcggcggcc gcccaaggcg atcgccgagg agctggccgg ctcgattggc
     3481 ggggtgccgg gagtggcgcg ggtggaggcc acgcgggggt acctgaacgt cttcctggat
     3541 cgcgggactt tcctgcgcgg ccggctggag ccgtcctcac cggagcctcc gcccgccgcc
     3601 ggtccgaaga cgatcgtcga gcacaccgcg atcaacccga acaaggcggc tcacatcggg
     3661 cacctgcgga acgcggcgct cggcgacacg ctggtgcgcg ccctgcggtt ccgcggcgag
     3721 cgggtcgagg tgcagaacta catcgacgac accggcgtcc aggttgccga cgtggtggtc
     3781 ggcttccgtg aactggaggg caaggggctc gaggaggtcc ggtcggtcgc cgggcagccg
     3841 cggttcgact actactgctg ggacctctac gcccgggttt ccgagtggta cgaggaagac
     3901 cgcgcccggc tcgaggctcg cggccgcgcg ctgcacgaca tcgagcacgg cggaaacgag
     3961 tcggccgaga ttgccgccct cgtcgccgac cggatcgtcc gctgccacct gcagacgatg
     4021 gcgcggatga acgtggacta ccacctgctc gcctgggagg gcgacatcct ccgcctgcag
     4081 ttctgggcgc gcgccttcga ggtgctgaag caggcggggg cggtctacct ccagtcacag
     4141 gggcggctgg cgggctgctg ggtcatggcg atcgacgacg gggacgcgcc cgaggcggtg
     4201 gccgaggccg gcggcctgga cgaggagcgc gagggggagc gcgagaaggt gatcgtgcgc
     4261 tccaacggaa ccgtgaccta cgtcggcaag gacatggcgt accagttctg gaagttcggc
     4321 ctcctcggcc gggacttcca ttaccgcccc ttcgagcggg acgccgatgg cgaggtgatc
     4381 tgggcgacct cgagcgtcga ggtggccggg ggcgaggcgc acccgccctt cggcggggcc
     4441 gcggccgtct acaacgtcat cgacgtccgc cagtcctacc tgcagaagct gctgaagcag
     4501 gccctggtcg cgatcggcca cccgcgcgag gcggaacggt cgtcgcactt ctcctacgag
     4561 atggtggcgc tgacccacaa caccgcccgg cagctggggt acgagctgtc ggccgaggac
     4621 gcggcccgtc cgttcgtcga ggtgtcgggg cgccgcggcc tcggggtgaa ggccgacgac
     4681 ctgctcgacg tgctggtcgc caaggccggc gaggaggtgg ggcggcgcaa ccccgacctg
     4741 gacgaggccg cggccgggaa gattgccagg gccatcgcga tcgccgcggt ccgctatttc
     4801 atgatccggt tctcgcgcgg gaaggtgatt gccttcgaca tcgacgaggc gctgaacttc
     4861 gagggtgaaa gcggcccgta cttgcagtat gctgtggtcc gcgccaacaa catcttccag
     4921 aagctccgcg accgcgaagg cataggcgaa gcggagttcc tgagccccct cggcggaacg
     4981 cccgagcagg aactggccgg cgaggtacac gggcacgatc tttgggcact ggtgctcgag
     5041 gccgcccggc tcgacgaggt ggtcgagcag gcggttcgga cgttggagtt ctcggtgctg
     5101 gccaagtacg cgtttggcct ggcccagatg ttcaacgcct tctaccaccg gtaccccatc
     5161 ctgaacgagg agcgtgacga tgcgcggcgg tggcgggcag ccgccgtcag ctactgccgg
     5221 aaccagctca ctgctgcgct cggactgatg gggatcccgg tcccgccaag gatgtagaag
     5281 ggcggccgtc ccgattcgag ggttgatatg tcgccgatcg tcggcatcac gccgtgccgg
     5341 agcctccccg actacctgga atcggtccgc cgggccggcg gagagccgcg cgtgctcgat
     5401 cccttgaagg atcggcccga ggcggtgatc tcggaactcg gcggcctgct gctgaccggc
     5461 ggcgtggacg tggatcccgc gcggtacggg gaggagcgcc agccgacggt gacctgtgtc
     5521 gagccggatc gggacgcgtt cgagttcggc ctgttcgacg cggcccgcgc cgccaacctg
     5581 cccgtgttcg ggatctgccg gggcatccag gtgatgaacg tggcgatggg cgggacgctg
     5641 gtgcaggaca tccccacgca gatggccggc agcctcgagc acaccatccc cgccccggcc
     5701 ttcgccgtgg cccacgagat ctgggtgtcg aagggcagcc ggctgtggac gctgatgcag
     5761 gagaagatgg ccgacggcga cggctgctcg gtgaacagcc gccaccacca ggccgtcaag
     5821 aaggtcgccc cgggcttcga ggtgacggcc accgcccccg acggggtcgt ggaggcgatc
     5881 gagtgcgcgc actccccctt ctgtctcggc gtgcagtggc acccggagaa cttctggcgg
     5941 accggagagt tccggccgtt gttcgagggc ttcatcgagg cgtgccgcgg ctgacgcggt
     6001 cgcgggacgc cgggcgggcc atcggaccgt gccgcccgga acgaggcggc atggaaacca
     6061 gagcgcggac ggtgtcactg gtgcgtcgag gcgtgaccaa cctgctggcg aagcggccct
     6121 tctgtatctc gttcgaggtc acctacaact gcaacgccca gtgcggccac tgccacctcg
     6181 ggggcgccgc caacaacccc cgggaagtcc gggcccccgc ggagcggttc ggcgagctgg
     6241 cccggcgctt caaaccggtg gtggcgcagg tatcgggcgg cgagcccctg ctccgggccg
     6301 acatcgacga catcgtctcc gcgatccgcg tccccaaccg cgccccgttc gtcgtgctga
     6361 cgaccaacgg cttcctgctg acgcgggagc ggtacgagcg gctgcggaag gccggcgtgg
     6421 acgagttctc gctgtcgttc gacttccccg acgaacgcca cgaccacttc cgtggcatcc
     6481 cggggctgtt ccggcgaatc gcgtcgctga tggaggggtt ccggccggat gaggccaagg
     6541 ccatcacgct gtcggcggtg gtgcaccgcg acaacttccg cgacctcgag gcgctggccg
     6601 agcaggcccg ggcgtggggc gtgcggatga acttctccac ctacacctgg ctgaggaccg
     6661 gcaagaagga gctgatgatc cccgccagcg agatggacga gttcaaggcg gtcatcgagc
     6721 ggctcatcgc gcacaagcgg cggcaccaga cgatttacac gtccgactac gtgttccgga
     6781 acatgatccg attcttcgag cacggggcgg tgccgggctg ccaggcgggc gtcaagttct
     6841 tcgtggtcaa cccggcgggg tcggtctcgc cctgtgggct catcatcaag aactacgagt
     6901 cgccggcaca gatccgcgaa gagttcctgg cacagaacga ctgcacggcg tgctacacct
     6961 ccatccgggg cggatccgaa cgccctctcc gctacctgat cggggataac ctcaaccgcc
     7021 ttcgccttcc cgcgacatcc atgtgaacga agcagtcgag ccgcaggtcg accgcgctcg
     7081 gcgccgccgc acgcgaatcc tgctcgccgc catcccggcc gcgttcctcg tcgggctcgt
     7141 cgggctgtgg attgccgcgc ccgacatccg ccagagcaag agcctgtggg tgctcttcct
     7201 ctactgcttc ccgtccgagt tcgtgatcgc ggtcgttccc cacgagcccg cgctgctgta
     7261 tttcgcgaag ttccacgacc cgtggacgat cgccggggta tcggcggtgg gaaccgtcct
     7321 gaccgagtgg gtgaactacc tggtggtcgc gtggattgcc gacgtgtcgt tcttccggaa
     7381 gctgattcgc ggcaaagcgg tgaccaggct gatcggctgg ttccaccgcg ccccctttgc
     7441 gacgctcctg gtggcgggga tcacgccggt cccgttctac ccgttccggt tcctcgccgt
     7501 catcggccgg tatccccttc ccaagtacct gctcgccgtc ctgatgtcgc ggacgccccg
     7561 cttctggatc atctgcgtgg cgggcgctgc gctggggttc tccgacgcgg tgatcgtagc
     7621 gtttgcgctg gtcctggccg ccgccggcct cgcgccggcg gcccgcgccc tgtggaggaa
     7681 gaagctccgg cccgcggcgc tcaaaaacga ctgaagaccc cgaccgcctc gacgtgagcc
     7741 gtgttgggga acagatcgaa cgcctcgaga tgctcgaggg cgtagccggc gtcgagcagg
     7801 cgccgcgcgt cgcggg
//
LOCUS       DAOPQX010000169         7802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10438_length_7802_cov_4.072931, whole genome shotgun sequence.
ACCESSION   DAOPQX010000169 DAOPQX010000000
VERSION     DAOPQX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_7802_cov_4.072931"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..845
                     /locus_tag="PKK95_10035"
     CDS             258..845
                     /locus_tag="PKK95_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18599.1"
                     /translation="MLKTLFASPRVTEPASVLLVRQPSSPVDPGAARDDVEAAGARVR
                     QIVEQLYGGRSLRIRAVDAGSTNAEEIELTALGNAYYDIERFGLSFVASPRHADILMV
                     SGPVTRNLERALRTTYEAAPHPCIVVAVGDGACDGGIWRGSYAVVGGVDAVIPVDIRI
                     PGDPPTPSEILRGLAEGLGRLPVAAGDGRREARGD"
     gene            838..2853
                     /locus_tag="PKK95_10040"
     CDS             838..2853
                     /locus_tag="PKK95_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC18600.1"
                     /translation="MTETLVQAGILAGLGGALLSGLVALVAGSARARAAAHALLAIAS
                     AAGAAAAFVFLAAGLAPLELGASPLFFGAPLAVDRLSSFFLLLVHLVVLLASWFGWRY
                     TELERHMYPARAVLPLTAIFVAGMQGVLLSTGLAAFLLFWEAMSVASFFLVMSDGRDE
                     SRRAALLYLAIAQFGAGALLVGMGLLSGGDVLATFATLGAATPSLGGSRAALATLLVV
                     FAFASKAGLVPLHAWLPEAHPRAPSHVSALMSGAMLKVAVYGLLRFTSACWPDLPAVW
                     GIGLLVLGLGGAAKAAIHANLDRDVKRVLAWSSVENVGLIFAMFGFSLVLRGRGLQAA
                     ADVVQAAMFLHILAHALFKSGLFLAAGATLTAVGSLKIEALGSLANRMPRLSAGALAL
                     SLAAASLPPFGAFAAEWLLLQGALVALAAGDPAAAVASAGVLLGLGTVAGLAVFAMVR
                     LFGFVFLAEPRSNAARAASEPGTALRGPVLAAAGLVLALGVASPWASPLLPRGIDGRV
                     LTVIPFPSENGMATLVSHADLLPVAGLALALAAAAAAATLAWRRLGRAGGTRRYHTWD
                     CGQPIDASMEYTATAFSAPVRHFLRDIVRAEKHVAFRPVVESNPWIRTGEMEFRRSAG
                     VLERVYYPIAGLIEAVGARLKQVQNGVIQFYIGLILATLLLALWVAL"
     gene            2850..3794
                     /locus_tag="PKK95_10045"
     CDS             2850..3794
                     /locus_tag="PKK95_10045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOC18601.1"
                     /translation="MNRILLTTVSGVTVVALAPLAAGLVRRFKAILQGRRGAPVLLPY
                     WTIATLLRKETVVSSSTTWVSRVVPVVVLACALVAAFALPLASRGGAAAPLSHLAAVA
                     GVWMLGAVFLVLGGIDSASAFGGMGASREMTIAAFLEPAIIVTLAAFGLAAGASTVDG
                     MLSVAGTGLVERPWLLPAVAALALVALGENSRYPVDNPATHLELTMVHEAMVLEYSGP
                     PLALLELAGMIKLATFTLLVANFVAPFGLATPAGGLLAILVAPVAAAAKLAAGMAGLA
                     LLESSMAKLRFYGLPEYFFGALFLGLTSLALGLIVGWL"
     gene            3791..4465
                     /locus_tag="PKK95_10050"
     CDS             3791..4465
                     /locus_tag="PKK95_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18602.1"
                     /translation="MNVDSLPVQDVLVGAMLLASFFSVGRKRLPTFLRYYAVSSACLA
                     GVIAASAIRTGHPAEYAGAMLTLIVKVTAIPAGLLLIARRSGELRLHPITRPVLSYAL
                     ALVALVAAYLMSRRVPLKVAAQAGAGLAQVWGLLFVALALILIGFLILIIRRDLYSQI
                     IGFLTLENGISAFAVVAIGGAPFLMEMGIFAVIASGVLLMAILSQQVHEIYRSHDTAV
                     LRDLTD"
     gene            4467..5942
                     /locus_tag="PKK95_10055"
     CDS             4467..5942
                     /locus_tag="PKK95_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC18603.1"
                     /translation="MALLGSFIVLLAAAAHAVAARDVRAALRGGVVAAVVAAAGLGGL
                     AVSVFARGTARPSPAWTIDRFGALMLLLVAAVYLGTALSSRRYIGAEHEAGILSSGRV
                     RLYCALIPLFVMAMVAAVATDHLGILWIALEATTLATTPLVALYRKDGAVEAAWKYLL
                     LCSVGISVSLLGLLLVAYAGVGAGLPVESALSLGALATRADALDPEVMRWAFVFLFVG
                     IGTKVGFVPMHPWLPDAHSRTPSPVSAALSGVLLNVALYGLLRAKALVDLALGTAGWT
                     SAFFWAFGLGSVLFAAFVLLHQQNYKRMLAYHSVEHMGLIAFGVAMGPAGAAGAVMHM
                     VGHTLAKSALFLSAGEILLHRHTTKIANIGQLWRTLPRTSLGFLLGFLGLIGAPTSVI
                     FASELTFLMAAARQRPYSAVALVVALAIVAVGVLYHVFAMLFSEGGRPVPEVRSAEPF
                     NLTHAVVAAELALLFAGGLFFLTSPGYGLAASIASTFTPLP"
     gene            5939..7510
                     /locus_tag="PKK95_10060"
     CDS             5939..7510
                     /locus_tag="PKK95_10060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC18604.1"
                     /translation="MNAETLLRSLTPVRAIRWSADRRPVGSVEPGDFASVATALHVDA
                     KLPLDLFFATDDRPHGLGFSLHAVFALDAEHEWLEIEADLPANRPHYPSLTRTLMAAN
                     WYERYAQDMFGVVAEGHPDPRRLVHHENVPGGTHPLRKDFAWDTRLERAEGRFPMNHV
                     DGEGVFEIPVGPIHAGIIEPGHFRFNVAGERIITLEGRLFFTHKGVEKLLEGRTPEAA
                     MPLVERVSGDSAASHALAFCTAVERAAGGRVPEAARLLRVLAVELERFTMHLHDVANI
                     CGMGTGFTVMAANGFRVKERLQRLSARLFGNRFFRGFIVPGGVARRPGDDELQDASRT
                     VEEAWQEVQALARLALDSDTLRDRLETTGVLGRDAARAYGAKGVAARASGLPRDARRD
                     HPNAAYADLPFGVVRTRNEGDVFARVRVRLEEMDDSRALVREVVNRCRTTSGGHRVPC
                     APTQGEGLGWCEGWRGQILDWVRLSGGAIDRAVIRDPSFCNWPLFAEIGPGNIVPDFP
                     LCNKSLNLSYSGTDL"
     gene            complement(7537..>7802)
                     /locus_tag="PKK95_10065"
     CDS             complement(7537..>7802)
                     /locus_tag="PKK95_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18605.1"
                     /translation="ADTAAFNRSLRRAVKPGGRVAVIDFPPRSFEHLDRPPAGTDGER
                     NGHGVSAQAVSREMERAGFVTERIDEDWGGRTYLVLLRAPGGP"
BASE COUNT          898 a         2856 c         2719 g         1329 t
ORIGIN      
        1 agcacctcca gcaccttcgg caccagcacc tccagcacct tcggcaccag cacctccagc
       61 accttcggca ccagcacctt cagcaccttc ggcaccagca ccttcagcac cttcggcacc
      121 agcacctcca gcaccccagc acctcctgca cctccagcac gacacggctc cctctcgccc
      181 cttgtgctat atttcgcttc ctcgcgcttg cgacgcctgc cggacccgtg ccggtcgaat
      241 ccatgtcctt tgtccgcttg ctcaagacgc tgttcgcctc ccctcgcgtg acggagcctg
      301 cctccgttct cctcgtgcgc caaccctcgt ctcccgtcga tccgggcgcc gcccgcgacg
      361 acgtcgaggc cgccggcgcg cgggtgcgac agatcgtcga gcagctctac ggcggacgct
      421 cgctccggat ccgcgccgtg gacgcgggct cgaccaacgc cgaggagatc gagctgacgg
      481 cgctcggcaa cgcgtactac gacatcgagc gcttcggcct cagcttcgtc gcgtcgccgc
      541 ggcacgccga catcctcatg gtgagcgggc cggtgacgcg caacctcgaa cgcgccctgc
      601 gcacgaccta cgaggcagcc cctcatccct gcatcgtcgt ggcggtgggg gacggcgcgt
      661 gcgacggcgg catctggcgc ggatcgtacg cggtggtggg gggagtggac gcggtgatcc
      721 cggtggacat ccggatcccg ggcgaccctc cgacgccatc cgagatcctg cgcggcctgg
      781 ccgaaggcct cggccgcctg ccggttgcgg cgggggacgg gcgcagggag gcacggggtg
      841 actgagactc tggtccaggc gggcatcctc gcgggcctcg gaggcgccct cctgtcggga
      901 ttggtcgccc tggtcgccgg ctctgcccgg gctcgcgccg ccgcgcacgc cctgctggct
      961 atcgcgtcag ccgccggcgc cgcagcggcc ttcgtgttcc tggcggccgg cctggcgccg
     1021 ctcgagctcg gcgcgtcgcc gctcttcttc ggcgccccgc tcgccgtcga tcggctctcg
     1081 tcgttcttcc tgctgctggt ccacctggtc gtgctgctcg cgtcctggtt cggctggcgc
     1141 tacacggagc tcgagcggca catgtacccc gccagggcgg tgctgccgct gacggccatc
     1201 ttcgtggccg gcatgcaggg ggtgctgctc tccaccggcc tcgcggcgtt cctcctcttc
     1261 tgggaggcga tgtcggtggc gtctttcttc ctcgtgatgt cggacgggcg cgacgagtcg
     1321 cgccgggcgg cgctgctcta cctcgccatc gcgcagttcg gcgcgggcgc cctgctggtc
     1381 ggcatgggcc tcctgtcggg cggcgacgtg ctggccacgt tcgcgacgct gggcgccgcc
     1441 acgccgtcac tcggcggcag ccgggccgcg ctcgccacgc tgctggtcgt gttcgcgttc
     1501 gcctccaagg cggggctggt gcccctgcac gcctggctgc ccgaggcgca cccgcgcgcg
     1561 cccagccatg tctccgcgct gatgtcgggc gccatgctga aggtggccgt ctacggcctg
     1621 ctgcggttca cgtcggcctg ctggcccgac ctgcccgcgg tgtgggggat cggcctgctc
     1681 gtgctcggcc tcggtggcgc ggcaaaggcg gcaatccacg cgaacctcga tcgcgacgtg
     1741 aagcgggtgc tggcctggag cagcgtcgag aacgtcggcc tgatcttcgc gatgttcggc
     1801 ttctcccttg tcttgcgggg acgtggcctg caggcggccg ccgacgtcgt ccaggccgcg
     1861 atgttcctgc acatcctcgc gcacgcgctc ttcaagtcgg gcctgtttct cgcggccggc
     1921 gccacgctga cggcggtggg ttcgctgaag atcgaggcgc tgggcagcct ggcgaaccgg
     1981 atgccgcgcc tctcggcggg cgcgctcgcc ctctcgctcg ccgcggcgtc gttgcctccc
     2041 ttcggggcgt tcgcggccga gtggctgctg ctccagggtg cgctggtggc gctcgcggcc
     2101 ggcgatccgg cagcggcggt cgcgtcggcc ggcgtcctgc tcgggctcgg caccgtcgcg
     2161 gggctcgcgg tgttcgccat ggtgcgcctg ttcggcttcg tctttctcgc cgagccgcgg
     2221 tcgaacgcgg cgcgcgccgc ctcggagccc ggcacggcgc tccggggacc ggtgctcgcg
     2281 gcggccggcc tggtcctggc cctaggcgtc gcgtcgccct gggcctcccc gctgctcccc
     2341 cgcgggatcg acggccgggt cctgacggtg atcccgttcc cgtcggagaa cggcatggcg
     2401 acgctcgtct cgcacgccga cctccttccc gtggccggcc tggcgctcgc gctggcggcc
     2461 gcggccgccg ccgcgacgct cgcctggcgg cgcctcgggc gcgcgggagg cacccgccgg
     2521 taccacacgt gggactgcgg gcagccgatc gacgcctcca tggagtacac cgccacggcg
     2581 ttctcagccc ccgtgcggca cttcctccgc gacatcgtgc gggccgagaa gcacgtcgcc
     2641 ttccggccgg tcgtcgagag caatccgtgg attcgcacgg gcgagatgga gttccggcgt
     2701 tcggcgggcg tgctcgagcg ggtctactac cccatcgcgg ggttgatcga ggcggtcggt
     2761 gcgcggctca agcaggtgca gaacggcgtc atccagttct acatcggcct catcctggcc
     2821 acgctgctcc tcgcgctctg ggtggcgctg tgaaccgcat tctcctgacc acggtctccg
     2881 gcgtgacggt ggtggccctg gcgccgctcg ccgcagggct ggtgcgccgc ttcaaggcca
     2941 tcctgcaggg ccggcgcggc gctcccgtgc tgctgcccta ctggaccatc gccacgctgt
     3001 tgcgcaagga gacggtcgtc tcgtcgagca ccacgtgggt gtcgcgggtc gtgcccgtcg
     3061 tcgtgctcgc gtgcgccctc gtcgcggcgt tcgcacttcc gctggcctcg cgcggcggag
     3121 cggccgcgcc gctttcgcac ctggcggcgg tcgcaggagt ctggatgctc ggcgccgtgt
     3181 tcctggtgct cggcgggatc gactcggcga gcgcgtttgg cggcatgggc gccagccgcg
     3241 agatgacgat cgcggcgttc ctcgaaccgg cgatcatcgt cacgctggcg gcgttcgggc
     3301 tcgccgccgg cgcgtcgacg gtggacggca tgctgtcggt ggcgggcacc ggcctggtcg
     3361 agcgcccgtg gctgctcccg gcggtggcgg cgctcgcgct cgtcgcgctc ggcgagaact
     3421 cgcgctaccc cgtggacaac ccggccaccc acctcgagct gacgatggtg cacgaggcga
     3481 tggtgctgga gtactccggc ccgccgctgg cgctgctgga gctggccggc atgatcaagc
     3541 tggcgacgtt caccctgctc gtcgccaact tcgtggcacc cttcggcctg gcgacgcccg
     3601 cgggcggcct gcttgcgatc ctggtggccc cagtcgcggc cgcggcgaag ctggctgccg
     3661 ggatggccgg tctcgccctg ctcgagtcga gcatggccaa gctgcgcttc tacgggctgc
     3721 ccgagtactt cttcggcgcg ctcttcctcg ggctcaccag cctcgcgctg ggcctgatcg
     3781 tggggtggct gtgaacgtcg actccctccc cgtgcaggac gtgctcgtcg gggcgatgct
     3841 gctggcgtcg ttcttctcgg tcggccggaa gcgcctcccc accttcctgc gctactacgc
     3901 cgtgtcttcg gcctgcctgg ccggggtgat cgcggcgagc gcgatccgga ccgggcaccc
     3961 ggcggagtac gccggcgcca tgctgaccct gattgtgaag gtgaccgcca ttcccgccgg
     4021 gctgctgctc atcgcgcgcc ggtcgggcga gctgcgactg cacccgatca cgcggcccgt
     4081 gctctcctac gccctggcgc tggtggcgct cgtggccgcg tacctgatgt cgcggcgggt
     4141 gccgctgaag gtcgcggcgc aggcgggcgc cgggctcgcc caggtctggg gcctgctctt
     4201 cgtcgcgctt gccctgatcc tgatcggctt cctgatcctg atcatccgcc gcgacctcta
     4261 ctcgcagatc atcgggttcc tgacgctcga gaacgggatc tcggccttcg ccgtggtcgc
     4321 gatcgggggc gcgccgttcc tgatggagat gggcatcttc gccgtcatcg ccagcggcgt
     4381 gctgctgatg gcgatcctca gccagcaggt gcacgagatc taccgctcgc acgacacggc
     4441 cgtgctccgc gacctgaccg actgacatgg cgctcctcgg atccttcatc gtgctcctgg
     4501 ccgccgcggc ccacgccgtc gctgcccgtg acgttcgcgc ggcgcttcga gggggcgtgg
     4561 tcgcggcggt cgtcgccgcg gccggcctcg gaggccttgc cgtctccgtc ttcgcccggg
     4621 gaaccgcccg gccgagcccg gcctggacca tcgatcgctt cggcgccctg atgctcctgc
     4681 tggtggcggc cgtctacctc ggcacggccc tcagcagccg gcgttacatc ggcgccgagc
     4741 acgaggcggg cattctctcg agcggccggg tacgcttgta ctgcgccctg ataccgctgt
     4801 tcgtgatggc gatggtcgcc gcggtggcca ccgaccacct cggcatcctc tggatcgcgc
     4861 tcgaggccac gacgctggcc acgacgccgc tggtggcgct ctacaggaaa gacggcgcgg
     4921 tcgaggcggc gtggaagtac ctgctcctgt gctcggtggg gatcagcgtc agcctgctgg
     4981 gcctgctgct ggtcgcctac gcgggcgtgg gcgcggggct cccggtcgag tcggcgctct
     5041 cgcttggcgc gctcgccacg cgggccgacg cgctggaccc cgaggtgatg cgttgggcgt
     5101 tcgtcttcct gttcgtgggc atcgggacga aggtgggctt cgtgccgatg cacccctggc
     5161 tgcccgacgc gcacagccgc acgccatcgc ccgtctcggc ggccctgtcg ggcgtgctgc
     5221 tcaacgtcgc gctctacggc ctgctgcggg cgaaggccct ggtggatctc gcgctcggaa
     5281 cggccggctg gacctccgcc ttcttctggg cgttcggcct cgggtcggtg ctcttcgccg
     5341 ccttcgtcct gctgcaccag cagaactaca agcggatgct ggcgtaccac agcgtcgagc
     5401 acatggggct catcgccttc ggcgtggcca tgggaccggc cggcgccgcc ggggccgtga
     5461 tgcacatggt cggccacacc ctggcgaaga gcgcgctgtt cctctcggcc ggcgagatcc
     5521 tcctccaccg gcacacgacg aagatcgcga acatcggaca gctctggaga acgctgccgc
     5581 ggacctcgct cgggttcctg ctcgggttcc tcgggctcat cggcgccccg acgtcggtca
     5641 tcttcgcgag cgagctgacc ttcctgatgg cggccgctcg gcagcgtccc tactcggccg
     5701 ttgccctggt cgtcgcgctc gccatcgtcg ccgtcggcgt cctttaccac gtgttcgcga
     5761 tgctgttctc ggaaggaggg cgcccggttc ccgaggtgcg gagcgccgag ccgttcaacc
     5821 tgacgcacgc ggtggtcgcc gccgagctgg cgctgctctt cgccggcggc ctgttcttcc
     5881 tcacctcgcc gggctacggc ctggcggcga gcatcgccag tacattcacg ccgctcccat
     5941 gaacgccgag accctgctcc gttcgctcac ccccgtgcgc gccatccgct ggtcggccga
     6001 ccggcgcccg gtcggcagcg tcgagccagg cgacttcgcg tcggtggcca ccgccctgca
     6061 cgtggatgcg aagctgcccc tggacctgtt cttcgcgacc gacgaccggc cgcatggcct
     6121 cgggttcagc ctccacgccg tgttcgccct cgacgccgaa cacgagtggc tcgagatcga
     6181 agcggacctg cccgccaatc gcccgcacta cccttcgctc acccggacgc tgatggcggc
     6241 gaactggtac gagcggtacg cccaggacat gttcggcgtg gttgccgagg gacaccccga
     6301 tcccaggcgg ctcgtgcacc acgagaacgt tcccggcggg acgcacccgc tcaggaagga
     6361 cttcgcctgg gacacgcggc tggaacgggc cgagggccgc ttcccgatga atcacgtgga
     6421 cggggaaggc gtcttcgaaa tcccggtcgg cccgatccac gcgggcatca tcgagccggg
     6481 ccacttccgc ttcaacgtcg ccggcgaacg gatcatcacg ctcgagggga gactcttctt
     6541 cacccacaag ggggtcgaga agctgctcga aggccggacg cctgaggcgg cgatgccgct
     6601 ggtggaacgg gtctcgggcg actcggccgc gtctcacgcg ctcgccttct gcaccgccgt
     6661 cgagcgggcc gccggcggcc gcgtgcccga ggccgcacga ctgctgcgcg tgcttgccgt
     6721 cgagctcgag cgcttcacga tgcacctgca cgacgtcgcg aacatctgcg ggatgggcac
     6781 cggcttcacc gtcatggccg cgaacggctt ccgcgtgaag gaacggctcc agcgcctgtc
     6841 ggcgcggctc ttcggaaacc ggttcttccg cggcttcatc gttccgggcg gcgttgcgag
     6901 gcggcctgga gacgacgaac tgcaggacgc gtcgaggacc gtggaggaag cctggcagga
     6961 agtgcaggcg cttgcccgcc tggcgctcga ctctgacacc cttcgagacc gcctcgagac
     7021 aaccggcgtg ttgggccgcg acgcggcgcg ggcctacggg gcgaagggag tagctgcgag
     7081 ggcatctggc ctgccccgcg acgcgcggcg cgatcacccg aacgccgcct atgcggactt
     7141 gccgtttggc gtggtgcgga cccgaaacga aggggacgtg ttcgctcgcg tcagggtgcg
     7201 gctcgaggag atggacgact cgcgcgcgct cgtgcgggaa gtggtgaacc ggtgccgaac
     7261 cacctccggc gggcaccgcg tcccctgcgc accaacgcag ggcgagggtc tcggctggtg
     7321 cgaggggtgg aggggtcaga tcctcgactg ggttcgattg agcggaggcg cgatcgaccg
     7381 ggccgtgatc cgcgatccct cgttttgcaa ctggccgctc tttgccgaga tcggtcccgg
     7441 caacatcgtt cccgacttcc cgctctgcaa caagtcactc aacctgagct actcgggcac
     7501 ggacctctag aaggctcacg gctcaaggct cagggctcac ggcccgccgg gtgcccgcag
     7561 caagaccagg tacgtccgcc caccccagtc ctcgtcgatc cgttcggtga cgaacccggc
     7621 gcgttccatc tcgcgcgaca ccgcctgcgc gctgacgccg tggccgttgc gctcaccgtc
     7681 ggtccccgcc ggcggccggt cgaggtgctc gaacgaccgc gggggaaagt cgatcaccgc
     7741 aacccggccg cccggcttga cggcccggcg aagcgaacgg ttgaaggcgg ccgtgtcggc
     7801 ga
//
LOCUS       DAOPQX010000170         7740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10524_length_7740_cov_3.907872, whole genome shotgun sequence.
ACCESSION   DAOPQX010000170 DAOPQX010000000
VERSION     DAOPQX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10524_length_7740_cov_3.907872"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PKK95_10070"
     CDS             <1..182
                     /locus_tag="PKK95_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18606.1"
                     /translation="AAGIIEAFADPTRGRALGARARALAERRYSDEAYIDRTRRALEA
                     FDPASALGAAAGSVS"
     gene            179..943
                     /locus_tag="PKK95_10075"
     CDS             179..943
                     /locus_tag="PKK95_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOC18607.1"
                     /translation="MTGERPADHYSYAVYADPATAECFDRRRFGGPIGTLVAATQEQL
                     LLSALPGVEGQAVLDVGTGTGRAALVLARRGARVIGLDASEQMLAVARSRAGAEGLGV
                     EFVVGDAHRLSYGDRAFDAAISLRLLMHAPDWRQCLGELCRVTRDRVVFDYPAWFSGA
                     ALQAMGRRVAAALGSRTEAYRVLRHRAVDRELARHGFRVIARHRQFVLPIALHKAIGS
                     RAFTLGTEKLMARGGLLRLLGSPVTVVAARDRDGAR"
     gene            940..1938
                     /locus_tag="PKK95_10080"
     CDS             940..1938
                     /locus_tag="PKK95_10080"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC18608.1"
                     /translation="MRPGTGDPVLVTGATGFTGGHLARGLAARNFRVRVLARDPEKAR
                     DLTTAGIEIAAGDLTSPASLECAVQGIATVYNVAAIYREAGVPASAYRAVNAEAVGAL
                     IRAAARAGVRRVVHCSTVGVHGDVENPPANEDAPLRPGDIYQETKLEGERVAREAAAA
                     SGIELVIARPSGIYGPGDRRLLKLFRGVARRRFVILGRGDIYYHLTYVDDLVEGFRLC
                     GTVPQAAGRTYILAGGEVGTLNELVHLIARESGVKPLPVRLPVWPFWMAGAACEAVCV
                     PLRVEPPIYRRRVDFFTKSRAFDISRARAELGYAPKVTLREGVRRSLEWYRLKGWI"
     gene            1980..2714
                     /locus_tag="PKK95_10085"
     CDS             1980..2714
                     /locus_tag="PKK95_10085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOC18609.1"
                     /translation="MTIPRVQDQLSDRRLTARQKYASLVVGRPGLGALLKYEATLLLS
                     QAVPGALGLALRKLLYPRLLGACGRGVVFGQNVVLRHPHKIRIADDVVIDDNCLLDAK
                     GEDNQGITIGPGVFIGRNTILSCKNGDIELGEAVNIGFNCEVFSASRVVIGRNALFAA
                     YCYVIGGDHDFSDPARAVAEQERHSLGVTVGEGVWMGAGAKVMDGVTIGAHAIVGAGA
                     VVKMDVPDRAVAVGRPAKIVGTRDLE"
     gene            2711..3754
                     /locus_tag="PKK95_10090"
     CDS             2711..3754
                     /locus_tag="PKK95_10090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC18610.1"
                     /translation="MSRILVVTSGLSFGAGGHLVIARSLARALREAGHQAEVRVTPQN
                     PFGHQASAYLSTWLTDVRTAQDGGPVDQVVSFRYPSYAVRHPRHVCWLNHRMREYYDL
                     WDGFHATLTPAQQAKETVRRRLIHGADRFLLTRNVKKLFAQSKTIQARLQRWGGIPSE
                     VLYPPPPQRAYRCEEYGDFIFAVSRLTKLKRFDMLVRAMARAEAGQTRAIIAGDGEAR
                     GDLEALARELGIDHRVRLVGEVSPDQLVDYLARCRAVCFPAFNEDFGFVTVEAFASGK
                     PVVTCTDSGGPAELVRDGVEGYVTAPNEEALGNALARLADDELLARELGANARQAVSS
                     MTWEAVVSKLVIV"
     gene            3760..4521
                     /gene="ttcA"
                     /locus_tag="PKK95_10095"
     CDS             3760..4521
                     /gene="ttcA"
                     /locus_tag="PKK95_10095"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOC18611.1"
                     /translation="MPYQSPLETRIAKRTTQAISDFQMLEEGDRVLVGLSGGKDSWAL
                     MQILDVLRRRAKMNFSLVAANVDAGFEGYDHELITRTCRERGWECIVRHTTINETMEN
                     VLDVDDTPCSLCGRLRRGVLYKMAGEIGATKIALGHHADDCIETLLLNLFFEGNLKAM
                     PAKLTSDNGQHVVIRPLVYVLEVEARAYAKECGLPIVPCSCPACGDLSLQRQRVKRLL
                     LDLEREHPGVKNSMLSALKNVTGSHLLDVRLQREP"
     gene            4523..4966
                     /gene="dtd"
                     /locus_tag="PKK95_10100"
     CDS             4523..4966
                     /gene="dtd"
                     /locus_tag="PKK95_10100"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOC18612.1"
                     /translation="MRLVLQRVSSARVTVDGEVVGEIGPGLLVLLGVTHGDTGAQAQH
                     LAGKIAGLRIFEDGQGKMNRSVAEAGGSVLVVSQFTLYGDCRKGRRPSFDAAAPADQA
                     RALYERFVEEVRAFGLPTATGVFQAHMKVELINDGPVTLMLEAGS"
     gene            5078..5935
                     /locus_tag="PKK95_10105"
     CDS             5078..5935
                     /locus_tag="PKK95_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC18613.1"
                     /translation="MATGHAATAETFTIGGDMTVRRLGFGAMRITGKGVWGPPADREE
                     AIRVLRRAVALGVNLIDTAESYGPYVSEELIAEALYPYPPGLVIATKVGYDRPGPNRW
                     SENCRPERIVEGLEGSLRRLRVERIDLYQLHRVDASVPEDDQFGTFQRLQREGKVRHI
                     GLSEVSVEQIERARRYFPVVTVQNRYNLVDREWEAVVDYCEREGIGFMPWRPVQAGRV
                     AQPGGRLQAIASNHGATRAQVAIAWLLHRSPVMLPIPGTSRVKHLEENLAAAGLRLTQ
                     EEVDRLTQG"
     gene            complement(5963..>7740)
                     /locus_tag="PKK95_10110"
     CDS             complement(5963..>7740)
                     /locus_tag="PKK95_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC18614.1"
                     /translation="TATDPLRQPRDTFDLSVRLDHAINRDHALRASYDRRSSTSRNLG
                     VGGYNLPDRAFESESATNTLRFSENGPLGRRMFTDTRLQVRWGDTTSRSSIEAPAIRV
                     QDAFTSGGAQQRGGASTTELEFATDLDYVRGAHSWRTGLLVEAGSYRADDVTNYLGTY
                     TFASLDDFRAGRPSAFTRRVGDPNVRYTTLQAAAYLQDDWRVARTLLLSAGIRYGVEQ
                     HVGDRWNLSPRATIAWSPFRSGRLTLRANYGYFYDWIPADLYKETVLVDAIRQRELNI
                     VNPGYPLEALGDLDDTVGTALLPSNRYLWPGGLALPGGHRLALGVERTLSENSRLIVS
                     YNRGWGLGLLRGRNLNSPVDGGRPDPRWANVIELAGDARSSSQQLSMVYSLVRMDLRR
                     LFFVANYSLSQARTNTAGAFAVAPGGDDLDAEWGPAAFDVRHRFGASLNLAPFRNVTM
                     GLNVRGQSGAPYTITTGRDDNGDGLFNDRPAGVGRNTVRGRAQWDLGGRIAYAIGFGT
                     PRQSGGGDGPRVVIRRGEGELGPGFGGGAEDKRYRVEFYLAGQNLLNRANYTAYSFVL
                     TSPFYGRPVAAAQPRKLQAGIRFGF"
BASE COUNT         1119 a         2625 c         2852 g         1144 t
ORIGIN      
        1 tggcggcggg catcatcgag gcgttcgccg acccgacccg cggccgcgcg ttgggcgccc
       61 gcgctcgtgc cctggccgag cgcaggtaca gcgacgaggc gtacatcgat cgcacgcggc
      121 gggcgctcga ggcgttcgat cccgcgtcgg ccctcggcgc cgcggccggg agcgtgtcgt
      181 gaccggcgag cgccctgccg accactacag ctacgcggtc tacgccgacc cggccaccgc
      241 ggagtgcttc gatcgtcggc gcttcggcgg tccgatcggc acgctggtcg ccgcgacgca
      301 ggagcagctg ctgctgtcgg cgctgccggg agtcgaggga caggccgtgc tcgacgtggg
      361 gacgggcacg ggacgtgccg cgctcgtgct cgccaggcgc ggggcgcgcg tcatcggcct
      421 ggatgcctcg gagcagatgc tcgcggtggc acggagccgc gcgggcgccg aggggctggg
      481 cgtggagttc gtggtgggcg acgcccaccg cctctcctac ggtgaccggg ccttcgacgc
      541 ggcgatcagc ctgcggctgc tgatgcacgc gcccgactgg cggcagtgcc tcggggagtt
      601 gtgccgggtg acgcgcgacc gggtggtgtt cgactacccg gcgtggttca gcggcgcggc
      661 cctgcaggcg atggggcgcc gtgtcgcagc ggcgctggga tcgcgaaccg aggcgtaccg
      721 ggtgctgcgc cacagggcgg tcgaccgcga actggcgcgc cacggtttcc gcgtgatcgc
      781 gcgccaccgc cagttcgtgc tgccgattgc cctgcacaag gcgatcggat cgcgcgcctt
      841 caccctgggt accgagaagc tgatggcacg gggcggcctc cttcgcctgc tcggctcgcc
      901 ggtgaccgtg gttgcggccc gcgaccggga cggggcgcga tgaggcccgg gaccggcgac
      961 ccggtgctcg tcaccggcgc caccggcttc acaggcgggc acctggcgcg cggcctcgcg
     1021 gcgcggaact tccgcgtccg ggttctcgcc cgcgatcccg agaaggcgcg cgacctcacg
     1081 accgccggca tcgagatcgc ggcgggcgat ctcaccagcc ccgcgtcgct cgagtgcgcg
     1141 gtgcagggga tcgcgaccgt ctacaacgtc gcggccatct acagggaggc cggcgtgccg
     1201 gcgtcggcct accgcgccgt gaacgcggag gccgtcggcg cgctcatccg cgcggcggca
     1261 cgggctggcg tgcgccgggt ggtgcactgc agcacggtgg gcgtgcacgg cgacgtggag
     1321 aatccgccgg cgaacgagga cgcgccgctg cggccgggcg acatctacca ggagacgaag
     1381 ctcgaagggg agcgcgtggc ccgcgaggcg gccgccgcgt cggggatcga gctggtgatc
     1441 gcgcgcccga gcgggatcta cgggccgggc gacaggcggc tgctcaagct gttccgcggc
     1501 gtcgcgcgcc ggcggttcgt catcctcggg cgtggcgaca tctactatca cctcacgtac
     1561 gtggacgacc tggtggaagg gttccggctg tgcgggacgg tgccgcaggc ggccgggcgc
     1621 acctacatcc tggccggcgg ggaggtgggg accctgaacg aactggtgca cctcatcgca
     1681 cgcgagtcgg gggtgaagcc gctgccggtc cgcctgccgg tgtggccctt ctggatggcg
     1741 ggggcggcgt gcgaggccgt ctgcgtgccg ctgcgggtgg agccgcccat ctaccggcgg
     1801 cgggtggact tcttcaccaa gagccgcgcc ttcgacattt cccgtgcgcg tgccgagctg
     1861 ggctacgcgc ccaaggtcac gctgcgcgag ggggtgcggc ggtcgttgga atggtatcgg
     1921 ttgaagggct ggatctgaga ttgggattct agattgagat tgggaatggg attgggacta
     1981 tgacaatccc ccgcgtccag gatcagctct ccgatcgccg cctgacggcg cggcagaagt
     2041 acgcctcgct ggtggtcggc cgccccggcc tcggcgccct gctgaagtac gaggcgacgc
     2101 tgctgctctc gcaggccgtg cccggcgcgc tcggtctcgc cctgcgaaag ctcctgtatc
     2161 cgcggctgct cggcgcttgc ggtcgcggcg tcgtcttcgg ccagaacgtc gtcctgcgcc
     2221 acccgcacaa gatccggatc gccgacgacg tggtgatcga cgacaactgc ctgctcgacg
     2281 caaaggggga ggacaaccag ggcatcacga tcgggcccgg cgtcttcatc gggcgcaaca
     2341 cgatcctctc gtgcaagaac ggcgacatcg agctgggcga agcggtgaac atcgggttca
     2401 actgcgaggt gttctcggcc agccgcgtcg tcatcgggcg caacgcgctg tttgccgcct
     2461 actgctacgt gatcggcggt gaccacgact tcagcgaccc ggcgcgcgcg gtggccgagc
     2521 aggagcggca ttcgctgggc gtcaccgtgg gcgagggggt ttggatgggc gccggcgcca
     2581 aggtgatgga cggcgtcacc atcggcgcgc acgcgatcgt tggcgcgggg gcggtcgtca
     2641 agatggacgt acccgaccgg gcggtggcgg tcggccgccc ggcgaagatc gtggggaccc
     2701 gggacctgga atgagccgca tcctcgtcgt cacctcgggc ctgtccttcg gggcgggagg
     2761 ccacctggtc atcgcccgct cgctggcgcg ggcgctgcgc gaggcgggcc accaagccga
     2821 ggtgcgggtc acgccgcaga acccgttcgg gcaccaggcc tccgcgtact tgtccacctg
     2881 gctcaccgac gtccggaccg cgcaggacgg cgggccggtg gaccaggtgg tgagcttccg
     2941 gtatcccagc tacgccgtgc gccacccgcg ccacgtctgc tggctgaacc accggatgcg
     3001 cgagtactac gacctgtggg acggcttcca cgccacgctg acgccggcgc agcaggcgaa
     3061 ggaaaccgtg cgccgccgtc tgattcacgg ggccgaccgc tttctgctga cccggaacgt
     3121 caagaagctg ttcgcgcagt cgaagacgat ccaggcgagg ctgcagcgct ggggcggcat
     3181 tccctccgag gtgctgtacc cgccgccgcc ccagcgcgcc tatcgctgcg aggagtacgg
     3241 cgacttcatc ttcgcggtct cgcgcctcac gaagctgaag cggttcgaca tgctcgtgcg
     3301 ggcgatggcc agggccgagg cgggacagac gcgggcgatc atcgcgggcg acggcgaagc
     3361 gcgcggcgac ctcgaggcgc tcgcccgcga gctgggcatc gaccaccgcg ttcgcctggt
     3421 cggcgaagtg agccccgacc agctcgtgga ctacctggcg cgctgccgcg ccgtctgctt
     3481 cccggccttc aacgaggact tcggattcgt gacggtggag gcgtttgcct cgggcaagcc
     3541 ggtcgtgacc tgcacggaca gcggcgggcc ggccgagctc gtgcgtgacg gcgtggaagg
     3601 gtacgtgacg gccccgaacg aggaggcgct cggcaacgct ctcgcccgcc tcgccgacga
     3661 cgagttgctg gcacgcgagc tgggcgccaa cgcccggcag gccgtgtctt cgatgacgtg
     3721 ggaggccgtc gtctccaagc tcgtgatcgt gtgaacgtga tgccctacca gtccccgctc
     3781 gagacgcgaa tcgccaagcg cacgacccag gccatctccg acttccagat gctcgaggag
     3841 ggcgatcggg ttctcgtcgg cctgtcgggc gggaaggact cctgggcgct gatgcagatt
     3901 ctcgacgtgc tgcggcgccg ggcgaagatg aacttctcgc tcgtcgccgc caacgtcgac
     3961 gccggcttcg agggctacga ccacgagctg atcactcgca cctgccgcga acgcggctgg
     4021 gagtgcatcg tccgccacac caccatcaac gagacgatgg agaacgtgct cgacgtggac
     4081 gacacgccgt gctccttgtg cggccggctg cggcgcggcg tgctctacaa gatggcgggg
     4141 gagatcggcg cgacgaagat cgccctcggg caccacgccg acgactgcat cgagacgctg
     4201 ctgctgaacc tgttcttcga ggggaacttg aaggcgatgc cggcgaagct gacctccgac
     4261 aacgggcagc acgtcgtcat ccggccgctg gtctacgtgc tcgaggtcga ggcgcgggcg
     4321 tacgcgaagg agtgcggcct gccgatcgtg ccgtgcagct gcccggcgtg cggcgacctg
     4381 agcctgcagc ggcagcgggt gaagcgcctg ctgctcgacc tcgagcgcga gcacccgggc
     4441 gtgaagaact cgatgctcag cgcgctcaag aacgtcaccg gttcgcacct gctggacgtc
     4501 cggctgcagc gggaaccgtg acatgcgtct tgtccttcag cgtgtttcat cggctcgcgt
     4561 caccgttgac ggggaagtcg tgggcgagat cggccccggc ctcctggtgc tgcttggcgt
     4621 gacgcacggc gacaccgggg cgcaggcaca acacctcgcc ggcaagatcg ccggcctgcg
     4681 catcttcgag gacggccagg gcaagatgaa ccgctcggtc gccgaggctg gcggcagcgt
     4741 gctcgtcgtc tcccagttca ccctctacgg cgactgccgc aaggggcgcc gcccttcctt
     4801 cgacgcggcc gccccggccg accaggcgcg cgccctctac gagcggttcg tcgaggaggt
     4861 ccgcgcgttc ggccttccca ccgccaccgg cgtcttccag gctcacatga aggtggagct
     4921 gatcaacgac ggaccggtga cgctgatgct cgaagcgggc agctgacagc aggcggctgc
     4981 cacccggcgg gcgcgatcct cgccttgcct caccctccga ccccggcgta ccctgaacaa
     5041 cgatccagtg tgacggtgta gcagccttgg aggcgacatg gcgaccggac acgcggcgac
     5101 agccgagacg ttcacgatcg gcggtgacat gaccgtccgg cgcctcggct ttggtgcgat
     5161 gcggatcacc ggcaaaggag tgtgggggcc gcccgcggac cgggaggagg cgatccgggt
     5221 gctgcggcgg gcggtggcgc tcggcgtgaa cctgatagac accgccgagt cgtacgggcc
     5281 ctacgtcagc gaggagctga tcgcggaggc gctctacccg tacccgccag ggctcgtgat
     5341 cgcaaccaag gtcggatacg accggcccgg tcccaacagg tggtcggaga actgccgtcc
     5401 cgagcggatc gtcgaggggc tggagggcag cctgcgccgc ctgcgggtcg agcggatcga
     5461 tctctaccag ctgcaccggg tggacgcgag tgtgccggag gacgaccagt tcggcacctt
     5521 ccagcggctt cagcgcgagg gcaaggtccg ccacatcggc ctctccgagg tgagcgtcga
     5581 gcagatcgag cgggcgcggc gctacttccc ggtggtcacc gtgcagaacc gctacaacct
     5641 ggtcgatcgc gagtgggagg cggtggtcga ttactgcgag cgcgagggga tcggcttcat
     5701 gccgtggcgt ccggtgcagg cggggagggt ggcgcagccg ggcggccgcc tccaggcgat
     5761 cgcgtccaac cacggcgcca cccgcgcgca ggtggcgatt gcctggctgc tgcaccgctc
     5821 accggtcatg ctgccgatcc ccggcacgag ccgcgtcaag cacctggaag agaaccttgc
     5881 ggctgccggc ctgcggctga cgcaagaaga ggtcgatcgc ctcacgcagg gctgagccga
     5941 ggacacgcgc cccggccttc cttcagaacc cgaaccggat ccccgcctgc agcttgcgtg
     6001 gctgggcggc ggccaccggc ctcccgtaga acggcgaggt caggacgaag ctgtaggccg
     6061 tgtagttcgc ccggttcagg aggttctggc ccgccaggta gaactccacc cggtaacgct
     6121 tgtcctcggc cccaccgcca aaccccggcc ccaactctcc ttcgccccgg cggatcacga
     6181 cacgcggccc gtcgccgccg ccgctctgtc tcggggtgcc gaagccaatc gcgtacgcga
     6241 tcctcccgcc caggtcccac tgcgcgcgcc cgcgaaccgt gttccgcccg acgcccgccg
     6301 gccggtcgtt gaacaggccg tcgccgttgt cgtcacggcc ggtcgtgatg gtgtacggcg
     6361 cgcccgactg tccccgcacg ttcagcccca tcgtcacgtt cctgaagggc gccaggttga
     6421 gcgacgcgcc gaagcggtgg cgcacgtcga aggccgccgg cccccactcg gcgtcgaggt
     6481 cgtcgccgcc aggcgccacg gcgaacgcgc cggcggtgtt ggtcctcgcc tggctgagcg
     6541 agtagttggc aacgaagaag agccggcgaa ggtccatcct caccaggctg tagaccatgc
     6601 tgagctgctg cgagctcgac ctggcgtcgc ccgccagctc gatcacgttc gcccaccgcg
     6661 gatccggccg cccgccgtct accggcgaat tcaggttgcg gccgcgcagc agcccgagcc
     6721 cccaaccccg gttgtagctg acgatgaggc ggctgttctc cgagagcgtg cgttcgacgc
     6781 cgagcgcaag ccggtgtcct ccggggagcg ccaggccgcc gggccagagg tagcggttcg
     6841 aggggagcag cgcggtgccc accgtgtcgt cgagatcccc gagggcctcg agcgggtatc
     6901 cggggttgac gatgttcagc tcgcgctgcc ggatggcgtc tacgagcacc gtctccttgt
     6961 agaggtcggc gggaatccag tcgtagaagt agccgtagtt cgcgcgaagc gtcaggcggc
     7021 cgctacggaa cggagaccag gcgatcgtgg cgcgcggcga caggttccag cgatcgccga
     7081 cgtgctgctc gaccccgtag cggatgcccg ccgagaggag cagggtgcgc gcgacgcgcc
     7141 agtcgtcctg gaggtaggcg gcggcctgca gggtggtgta gcggacgttc ggatcgccga
     7201 cgcggcgcgt gaaggcggag gggcggccag cccggaaatc gtccaggctg gcaaaggtgt
     7261 aggtgccgag gtagttcgtg acgtcgtcgg cgcgatacga gccggcctcg acgagcagcc
     7321 cggtgcgcca cgagtgcgcg ccgcgcacgt agtcgaggtc ggtggcgaac tccagctccg
     7381 tcgtcgaggc gccgccgcgc tgctgcgccc cgccgctcgt gaaggcgtcc tgaacgcgaa
     7441 tcgcgggcgc ctcgatcgac gagcgcgacg tggtgtcgcc ccagcggact tgcagccgcg
     7501 tatcggtgaa catccggcgc ccgaggggac cgttctcgga aaaccgaagg gtgtttgtgg
     7561 ccgactcgct ctcgaaggcc cggtcgggca ggttgtagcc gcccaccccc aggttgcgcg
     7621 aggtggagga ccggcggtcg tagctcgcgc gcagcgcgtg atccctgttg atcgcgtgat
     7681 cgagccgcac gctcaggtcg aaggtgtcgc ggggctggcg gagggggtcg gtcgccgtac
//
LOCUS       DAOPQX010000171         7731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10552_length_7731_cov_5.161412, whole genome shotgun sequence.
ACCESSION   DAOPQX010000171 DAOPQX010000000
VERSION     DAOPQX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10552_length_7731_cov_5.161412"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..677)
                     /gene="pyrE"
                     /locus_tag="PKK95_10115"
     CDS             complement(96..677)
                     /gene="pyrE"
                     /locus_tag="PKK95_10115"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOC18615.1"
                     /translation="MNRDDLLDLFRRCSALLEGHFRLSSGLHSPGYLQCALVLQHPRH
                     AATLGEELAAPLRELRPATVLSPALGGLIIGHEVARALGARAIFAEREDGVLRLRRGF
                     TLTEAERVVVVEDVVTTGGSTRETMQVARAAGAQVLAAGAVVDRSGGVTSLDVPLHAL
                     VSLSLPTYEPAACPLCAQGVPLTKPGSRQVAVR"
     gene            771..1928
                     /locus_tag="PKK95_10120"
     CDS             771..1928
                     /locus_tag="PKK95_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC18616.1"
                     /translation="MKERPADLAVNGGTPAFAETLHVGRPNIGDRARFLERAAAILDR
                     RWLTNDGDMVRAFEREVAAACGVPHVVAVCNGTMALALACRALGLTGEVIVPSFTFVA
                     TAHVLEWHGLVPVFCDVDPATHTLDPDRVEELLSDRTSAILGVHLWGRACDVERLRTL
                     ADRRRLALLFDAAHAFACHRDGVPVGSFGDAAALSFHATKIVNAFEGGAVATRDGELA
                     ARLRLLRNSGFAGIDRVAAVGLNAKMTEVSAAMGLTSLESLAVFLEANRANYAAYVSN
                     LDGLPGIRLVDTGTSRAHVVIEVDQEVCGVSRDRLVEILWAENVRARRYFHPGCHRQA
                     PYAERYPAAGARLPVTERLAEQVLVLPTGTAVTPGDVAEVCSIVRVATGER"
     gene            complement(1972..4410)
                     /locus_tag="PKK95_10125"
     CDS             complement(1972..4410)
                     /locus_tag="PKK95_10125"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC18617.1"
                     /translation="MSRRSIPGLFALAVLVALLAGRPSAAPAGFGGQPAAPRPAEAPA
                     RFALTVESIMRGPALVGYPPSGLRWAGDSSELYFEWRRPGDDEAATYAVRREGGEPRK
                     LGEDERKLVPPPSGGTWDDARRRVLFVESGDIVLLDTATRSRLRITRTAGAESNPRWA
                     RGETAVTFVRDGSLFLAPLGSGPVEQIVEVRPRKRETRETESQTYLRAEEEKLIGFTR
                     EAIRKRKAAEEKREGEAPPVFEIQEGQSVADAAVTPGGHVFLLVNERPSGAKTADTPN
                     YVTESAYTELIPGRAKVGDAQGRRRLAILNLATRRAVWAESSFAGKRQVDGAKGGEGR
                     NVDREVGWAMPLFSPDGALAVAWVRAADFKDRWIVAVDPESGAARVVDAHHDDAWVSE
                     GGFGPLGVRNAGFLPDGRRIYFTSEKDGWMHLHVVDASTPGARAVSLTRGKWEVSAVT
                     LSPDRTRFYFVSSERHPGERHLCVMPIEGGEWMQLTSAPGSQAGEVSPDLRTIALVSS
                     YSNKPPEVYLMPNEKGAVPRQVTTTPTGEWRAFKWADPRVITFKARDGAEVHARLYTP
                     EMLGARRDPAHPGVVFVHGAGYTQNAHRWWSSYYREYMFHNLLASLGYVVLDVDYRAS
                     AGYGRDWRTAIYRHMGGKDLDDIVDGARYLVASERVDPRRLGVYGGSYGGFITLMALF
                     TSPDTFAAGAALRPVTDWAHYNHGYTGSILTLPQDDPEAYRKSSPIYFAEGLKSALLI
                     CHGMVDTNVHFQDSVRLVQRLIELGKDNWELAVYPVENHGFEQQSSWVDEYRRILALF
                     ERNLRGRGGPCNRP"
     gene            4544..6229
                     /locus_tag="PKK95_10130"
     CDS             4544..6229
                     /locus_tag="PKK95_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC18618.1"
                     /translation="MGLTKFRIFAASSAVTLALAVPVSAQSLQQAAPAQAEAAQAPGP
                     VKRLSVADAVQLALEQNLNLQVQRLDPQVQDLAISQVRSAWTPLFTTTLVTSNSKSPV
                     NSFLSGATDKLTNDSFQAIVSAQQQLPWGGGSFNIDWNNSRTENNSAFSSPNPSLRGE
                     ISLTYTQPLLRNFRIDSVRQQLLVSRKNREISDVQLQQAVLTTTRNVKSAYWDLAYAI
                     NSLSVQRQSLELAQESLRNNRSRVEIGTMAPIDIVEAEAEVAQREEAVLLAEAAVTRA
                     EDALRALVFDPTTPDFWSMRLELTDEPTFQDQAIDLTAAVSNAMANRTDLLQARKNIE
                     ASDISIRYFRNQILPDVNLQAGYGVTGQGGTQYNFGDGFPPVVMGRIDEGYGKVLSRM
                     FGRDFPNWSVALQVGYPLGRSNAEAGLARARIQYQQSQIQLRNLELQVATQVREVARQ
                     VNTNRKRVDSTRAARQLAERRLEAEQKKFAAGMSSSFFVIQAQRDLAQARNAELQAIL
                     DYNRSLVDFETVQQAPVGGTGAMVSVVSAGGGGSGSSISSGSQQNSGRGPGGF"
     gene            6257..7006
                     /locus_tag="PKK95_10135"
     CDS             6257..7006
                     /locus_tag="PKK95_10135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC18619.1"
                     /translation="MAGLTVTIITRDEVDRLEEAVASAAFADEIVIVDSGSSDGTLEL
                     ARRLTPRVIVRDWPGFAAQKNFAASQASHDWIFSLDADERVSPALAAEIADLVAAGPR
                     FSAYRVPRVSSYLGRWIRSTDWYPDYQVRLYDRRAASWGGEYVHESVRAAGEVGRLRG
                     ELLHLPYRDISHHLRTIDCYTELSARQMRDEGRRVTLAGLAFRPAGAFLRNYVLRRGF
                     RQGGVGLIVSMLNSYYVFLKFARLWTATRER"
     gene            7003..>7731
                     /locus_tag="PKK95_10140"
     CDS             7003..>7731
                     /locus_tag="PKK95_10140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC18620.1"
                     /translation="MITLQIDTARGWRGGQNQVLLTARGLRARGHRVVVMAHPEGELR
                     RRVEGEFEVLALAPRGEVDLKAAWRLAGALRALEPDIVHAHDPHGVAVAAAALPISGL
                     ARRPPLVAARRVDFHLKANSFSRWKYRQVDAFLTSSDAIRAMLVEDGIAPERVTTVYE
                     GVDVDRIAAIDPIDLHGEYWFPAQAPIIGNVAALVAHKGQRYLLDAAAIVVREVPHAR
                     FLILGEGELRQELEHRLRHLKLDQH"
BASE COUNT         1131 a         2814 c         2651 g         1135 t
ORIGIN      
        1 gagcaggccc tggatggacg ttccctcggc ctgccgctgc cagccgacga accgggtgcc
       61 gtcgtaggcg aggacgatct tcagcgtgcg cccgctcacc tgacggcgac ctgccgcgat
      121 ccgggcttcg tcaggggcac gccctgcgcg cacagcgggc acgcggccgg ctcgtaggtc
      181 ggcagtgaga gcgacaccag cgcgtgcagc gggacgtcga gggaggtgac tccgccgctc
      241 cggtccacga cggcccccgc cgccagcacc tgcgcccccg ccgcgcgcgc cacctgcatc
      301 gtctcgcgag tggagccgcc cgtcgtgacc acgtcttcga ccacgacgac gcgctcggcc
      361 tcggtgagcg tgaacccccg ccgcaggcga agcacgccat cctcgcgctc ggcgaagatt
      421 gcccgcgccc caagcgcccg cgccacctcg tggccgatga tcaggccccc gagggcaggc
      481 gagagcacgg tggcggggcg gagctcgcgc agcggcgcgg ccagctcctc cccgagcgtg
      541 gcggcgtgac gcgggtgctg cagcacgagc gcgcactgca ggtacccggg gctgtgcagc
      601 ccggacgaaa ggcggaagtg gccctcgagc agcgccgagc agcgacgaaa caggtcgagc
      661 aggtcgtcac ggttcatgcg gtcatcctag tccaatcccg attccaatcc caatcccgat
      721 ccgaatctcc cctgttgcgc ccgcctgacc cgttccggta tacgaagagc gtgaaggagc
      781 gacctgcaga ccttgccgtc aacggcggca ccccggcgtt tgccgaaacc ctgcacgtgg
      841 gccggcccaa catcggcgac cgggcacggt tcctcgagcg cgccgcggca atcctcgacc
      901 gccggtggct caccaacgac ggcgacatgg tgcgcgcgtt cgagcgcgag gtggccgccg
      961 cgtgcggggt gccgcacgtc gtggctgtct gcaacggcac catggcgctg gcactggcct
     1021 gccgcgccct cgggctgacc ggcgaggtga tcgtcccgtc gttcaccttc gtcgcaacgg
     1081 cccacgtcct cgagtggcat ggcctcgttc ctgtcttctg cgatgtcgac cccgcaaccc
     1141 acaccctcga tcccgatcgg gtggaggaac tcctctcgga caggacctcc gccatcctcg
     1201 gcgtgcacct ctggggccgg gcgtgcgacg tcgagcgatt gcggacactg gccgatcgcc
     1261 gtcgcctggc cctgctcttc gacgccgcgc acgccttcgc ctgccatcgc gacggcgtgc
     1321 cggtgggatc gttcggcgac gcggcagccc tgagcttcca cgcgacgaag atcgtcaacg
     1381 ccttcgaggg gggcgccgtg gccacccgcg acggcgagct cgcggcccgc ctgcgactcc
     1441 tcaggaacag cgggttcgcg ggaatcgatc gcgtggccgc ggtcggcctc aacgcgaaga
     1501 tgaccgaggt gtcggcggcg atggggctca ccagcctcga gtcgctcgcc gtcttcctcg
     1561 aggccaatcg cgccaactac gccgcgtacg tctcgaacct cgacggcctc cccggcatcc
     1621 gtctcgtgga caccggcacc agccgcgcgc acgtcgtcat cgaggtcgac caggaggttt
     1681 gcggcgtctc ccgcgaccgg ctcgtggaga tcctgtgggc cgagaacgtg cgggccaggc
     1741 ggtatttcca ccctggctgc catcgccagg cgccgtacgc cgagcgctac ccggcggccg
     1801 gcgcgcgcct gccggtcacc gagcgcctgg cggagcaggt gctcgtgctg ccgaccggaa
     1861 ccgccgtcac cccgggcgac gtggcggagg tctgcagcat cgttcgcgtg gcgactggcg
     1921 aacggtaggt gcggccgaga gcgcctcgtc gcaggcggtc actcgggcgc gtcaaggccg
     1981 gttgcagggg ccgcccctcc cgcgcaggtt ccgttcgaac agggccagga tccggcggta
     2041 ctcgtccacc cagctgctct gctgctcgaa cccgtggttc tcgaccgggt agacggccag
     2101 ctcccagttg tccttgccga gctcgatcag acgctgaacc aggcgcacgg agtcctggaa
     2161 gtggacgttg gtgtccacca tcccgtggca gatgagcagg gcgctcttga gtccttccgc
     2221 gaagtagatg ggagagctct tgcgatacgc ctcgggatcg tcctggggca gggtgagaat
     2281 gcttcccgtg tacccatggt tgtagtgagc ccagtcggtg acgggcctga gcgcggcgcc
     2341 ggcggcgaac gtgtcgggcg aggtgaagag cgccatcagg gtgatgaagc cgccgtaact
     2401 gccgccgtac acgccgagcc gcctggggtc cacgcgctcg gacgcgacga ggtaccgggc
     2461 gccgtccacg atgtcgtcca aatccttgcc gcccatgtgc ctgtagatcg ccgtccgcca
     2521 gtcgcgcccg tagccggcgc tcgcacggta gtccacgtcg aggacgacgt agccaaggct
     2581 cgcgaggagg ttgtggaaca tgtactcgcg gtaataactc gaccaccagc ggtgcgcgtt
     2641 ctgcgtgtag cccgcgccgt ggacgaagac gacgccgggg tgagcggggt cgcggcgggc
     2701 gccgagcatc tccggcgtgt agagccgtgc gtgcacctcc gcgccgtcgc gcgccttgaa
     2761 ggtgatcacc cgggggtccg cccacttgaa cgcgcgccac tccccggtcg gcgtggtggt
     2821 cacctgccgc ggcaccgccc ccttctcgtt cggcatcagg tacacctcgg gcggcttgtt
     2881 gctgtaggag gagaccaggg cgatcgtgcg caggtcgggt gagacctcgc ccgcctgcga
     2941 tccgggcgcg gaagtcaact gcatccactc cccgccctcg atcggcatga cgcacaggtg
     3001 gcgctcgccc gggtggcgct cgctcgacac gaagtagaag cgggtgcgat cgggagacag
     3061 cgtcacggct gacacctccc acttgccccg cgtcagcgac accgcccggg cgccgggcgt
     3121 ggacgcatcc accacgtgaa ggtgcatcca gccgtccttc tcggaagtga agtagatgcg
     3181 ccggccgtcg gggaggaacc ccgcgttgcg cacgccgagg gggccgaagc ctccttccga
     3241 aacccacgcg tcgtcgtggt gggcgtcgac cacccgcgcc gcgccgctct cgggatcgac
     3301 cgcgacgatc catcggtcct tgaagtcggc ggcacgcacc caggcgacgg ccagcgcgcc
     3361 gtccggcgag aagagcggca tggcccatcc cacctcgcgg tccacattcc gcccctcgcc
     3421 tcccttcgcg ccatccacct gccgcttgcc ggcgaacgac gactcggccc agacggctcg
     3481 ccgggttgcc aggttgagga tcgcgagccg ccggcggccc tgggcgtcgc caaccttcgc
     3541 ccggcccggg atcagctcgg tgtaagccga ctcggtcacg tagttgggcg tgtcggcagt
     3601 cttcgctccg ctcggccgct cgttgacgag caggaagacg tgcccgcctg gcgtcaccgc
     3661 ggcgtcggcc acgctctggc cctcctggat ctcgaagacg ggcggggcct ccccttcccg
     3721 cttctcctcg gccgccttgc gcttccgaat cgcctcgcgc gtgaacccga tgagcttctc
     3781 ctcttccgcc ctgaggtatg tctggctctc ggtctcgcgg gtctcgcgct tccgcggccg
     3841 gacttcgacg atctgctcga cgggccccga gcccagcggc gcgaggaaca ggctgccgtc
     3901 gcgcacgaag gtgacggcgg tctcgccgcg cgcccaccgg gggttcgact cggcccccgc
     3961 ggtccgggtg attcgcagcc tgcttcgtgt cgcggtgtcc agcaggacga tgtcgcccga
     4021 ctcgacgaag aggacgcggc gccgcgcgtc gtcccacgtg ccgcccgacg gcggcggcac
     4081 gagcttccgt tcgtcctcgc cgagcttcct cggctcgccg ccctcgcgcc gcaccgcgta
     4141 ggtggccgcc tcgtcgtccc ccggccgccg ccactcgaag tacagctcgc tcgagtcgcc
     4201 ggcccaccga agcccgctcg gggggtagcc gacgagcgcg ggaccgcgca tgatgctctc
     4261 gacggtcagc gcgaagcggg caggggcctc ggccggccgg ggagcggccg gctggccccc
     4321 gaagcccgcc ggagcggcgg atgggcggcc ggccagcagg gcgaccagga ccgcgagcgc
     4381 gaacaggccg gggatggaac gtcgggacat gggtagaacc tcggagcgct ggtgggaggc
     4441 ggaggggggc cggcttggag cggtagtata caattccgcg gccggcagaa ccggcggcgc
     4501 ctaccgttcg tctactactg agaccagcac tggagtcatc tagatgggtc tgaccaagtt
     4561 ccgcatcttc gccgcttctt ccgccgtcac gctggccctg gccgtcccgg tatccgccca
     4621 gtcgctgcag caggcagccc cggcgcaggc cgaggccgcg caggcccccg ggcccgtcaa
     4681 gcggctgtct gtggccgacg ccgtgcagct ggcgctcgag cagaacctga acctccaggt
     4741 ccagcggctc gatccccagg tccaggacct ggcgatcagc caggtgcggt ccgcctggac
     4801 gccgctcttc acgacgaccc tcgtcaccag caacagcaag agcccggtca acagcttcct
     4861 ctccggcgcc accgacaagc tgaccaacga cagcttccag gccatcgtct cggcgcagca
     4921 gcagctgcca tggggcggcg gcagcttcaa catcgactgg aacaacagcc ggacggagaa
     4981 caactccgcc ttctcgagcc cgaacccctc gctgcgcggg gagatcagcc tcacctacac
     5041 gcagcccctg ctccgcaact tccggatcga cagcgtccgc cagcagctgc tggtgagccg
     5101 gaagaaccgc gagatctccg acgtgcagct gcagcaggcg gtgctcacca cgacgcgcaa
     5161 cgtgaagagc gcctactggg acctggcgta cgcgatcaac tcgctcagcg tccagcgcca
     5221 gtcgctcgag ctggcgcagg agtcgctgcg aaacaaccgg tcgcgcgtcg agatcggcac
     5281 catggccccg atcgacatcg tcgaggcgga ggccgaggtg gcgcagcgcg aggaggccgt
     5341 gctgctggcg gaggccgccg tcacccgtgc cgaagacgcc ctgcgcgccc tcgtcttcga
     5401 ccccacgacc ccggatttct ggtccatgcg cctcgagctc accgacgagc cgaccttcca
     5461 ggaccaggcc atcgacctca cggccgccgt ctccaacgcg atggcgaacc gcaccgacct
     5521 gctgcaggcg cggaagaaca tcgaggcgag tgacatcagc atccgctact tccgcaacca
     5581 gatccttccc gacgtcaacc ttcaggcggg ctacggtgtg acgggccagg gcggcacgca
     5641 gtacaacttc ggcgacgggt tccctcccgt cgtgatgggg cggatcgacg agggctacgg
     5701 caaggtgctc tcgcggatgt tcgggcgcga tttcccgaac tggtcggtgg cgcttcaggt
     5761 gggctacccg ctcggccgca gcaacgccga ggcggggctg gcgcgggccc gcatccagta
     5821 ccagcagtcg cagatccagc tgcggaacct ggagctgcag gtggcgacgc aggtccgcga
     5881 ggtcgcccgg caggtgaaca ccaaccgcaa gcgtgtcgac tccacgcgcg cggcgcggca
     5941 gctggccgag cgccggctcg aggccgagca gaagaagttc gcggcgggca tgtcgtcgag
     6001 cttcttcgtc atccaggcgc agcgcgatct cgcccaggcg cgcaacgccg agctgcaggc
     6061 catcctcgac tacaaccggt cgctcgtcga cttcgagacg gtgcagcagg cgcccgtggg
     6121 cggcacgggt gcgatggtct cggtggtgtc ggcgggcggc gggggctccg gctcgtccat
     6181 ctcctcgggc tcgcagcaga actcgggccg gggtcctgga gggttttagg gccggatctg
     6241 ggctacactc cccagcgtgg caggcctgac ggtcacgatt atcacgcgcg acgaggtcga
     6301 ccgcctcgaa gaggcggtcg cctcggccgc cttcgccgac gagatcgtca tcgtcgactc
     6361 gggcagttcg gacgggacgc tcgagctcgc tcgccgcctc accccgcgtg tcatcgtccg
     6421 cgactggccc ggcttcgccg cgcagaagaa cttcgccgcg agccaggcct cgcacgactg
     6481 gatcttctcg ctcgacgccg acgagcgggt ctcgccagcc ctcgcggccg agatcgcgga
     6541 cctcgtcgca gcggggcccc gcttctcggc ctaccgcgtg cctcgcgtga gctcctacct
     6601 ggggcgctgg atccgctcga cggactggta ccccgactac caggtccgcc tctacgaccg
     6661 gcgcgcggcg tcgtggggtg gagagtacgt gcacgaaagc gtgcgcgccg cgggcgaggt
     6721 gggacgcctg cgtggcgagc tgctccacct cccgtaccgg gacatctcgc accacctgcg
     6781 aaccatcgat tgctacaccg agctgtcggc ccggcagatg cgcgacgagg ggcgtcgggt
     6841 gacgctggcc ggcctcgcgt tccggccggc gggcgcgttc ctgcgcaact acgtcctgcg
     6901 ccgcggcttc cggcagggag gggttggcct gatcgtctcg atgctcaaca gctactacgt
     6961 gttcctgaag ttcgcccgct tgtggacggc aacgcgcgag cgatgattac ccttcagatc
     7021 gacacggccc gcggctggcg gggcggccag aaccaggtgc tgctcaccgc gcgcggcctc
     7081 cgcgcgcgcg gccaccgcgt cgtcgtgatg gcccaccccg agggcgagct gcgccgccgc
     7141 gtcgagggcg agttcgaggt gctggcgctg gcgccgcgcg gcgaggtgga cctgaaggca
     7201 gcctggcgcc tggccggcgc cctgcgcgcg ctcgaacccg acatcgtcca cgcgcacgat
     7261 ccccacggcg tcgccgtggc cgccgccgcg ctgccgatca gcggcctcgc ccgccggccg
     7321 ccgctcgtcg cggcgcgccg ggtcgacttc cacttgaagg cgaactcgtt ttcccggtgg
     7381 aagtaccggc aggtggacgc cttcctcacc tcgtcggacg cgatccgcgc gatgctcgtc
     7441 gaggacggca tcgcccccga gcgcgtgacg acggtgtacg aaggggtgga cgtcgatcgg
     7501 atcgcggcga tcgacccgat cgacctgcac ggcgagtact ggttcccggc gcaagccccg
     7561 attatcggca acgtcgccgc cctggtcgcc cacaagggcc agaggtacct gctcgacgct
     7621 gcggcgatcg tcgtgcgcga ggtgccccac gcccgcttcc tgatcctcgg cgagggcgag
     7681 ctgcgccagg agctcgaaca ccgccttcgc cacctcaagc tcgatcagca c
//
LOCUS       DAOPQX010000172         7714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10578_length_7714_cov_3.768899, whole genome shotgun sequence.
ACCESSION   DAOPQX010000172 DAOPQX010000000
VERSION     DAOPQX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10578_length_7714_cov_3.768899"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKK95_10145"
     CDS             <1..326
                     /locus_tag="PKK95_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18621.1"
                     /translation="GTRFPFCNYQSGKIWGTPADEPGAAPDPRQVVEASWPMLLSDLE
                     LRAPAFVLDAAAAGLDRWAGHEIARYPRLASAVQERYRLVAVVSGVAIYQRRESVETS
                     SVRHP"
     gene            323..1777
                     /locus_tag="PKK95_10150"
     CDS             323..1777
                     /locus_tag="PKK95_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC18622.1"
                     /translation="MIRRVSQAVVVLADGVGASFLQPLIGTGEYPALDRLAAVGGPGG
                     PRAASSVFPTTTGPAHLPFITGRFPGPCNIPGIRWFDPGRYGRHAFGSGRFRSYMGPG
                     SYLASRDLAREVATLFELVADHACIGGHARRGVGPGRNLTRWSKAATSVLSFVSEDWT
                     RSDRIAARRLVEAVDRGTRLVFASFYGGDAAAHKWGKGPQVREGCRRIDSAIGEVLSR
                     LESNGRADQTLLLVVSDHGFSDTTAHLDLHRLVQDAIGPTLAHPQVWRGYFGGAAAAV
                     MVSGNAMAHVYLRGAGWERSPALDPPGPEASRLLDALLREPAVDQVILRGSPTAAIVA
                     SARGRARICRHEGRILYAPEAGRDAFGYDAGLAGCRDEDGWLTATWDTDYPDAPVQIL
                     QLLASPRAGHLLVTARPGFDLRARFEKPPHRGSHGALHRLHVMTPLLSTHPLRSGPVR
                     TADVMPTVLDALGEPVPPGLDGRSLWPDSRGDGA"
     gene            1774..4587
                     /locus_tag="PKK95_10155"
     CDS             1774..4587
                     /locus_tag="PKK95_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOC18623.1"
                     /translation="MTRRIRHARSARVLAAACTVSGIVCLAAALVAAAGGASGDTAPR
                     HAADEWIDPAYNVVMPPGGIVALDASADRFAVEVLRRERSSTVTSVHLALTIRGRGAV
                     VGGGEHRAAIADGRHALVSVPAPMSPWTILANGRLAAFVRAWEYGGFQIDAEVREGGP
                     GGRLVYRLHDPAALVRISRRGFFDRYGLTLCLAGLAAALLASYASARLLGGTGWRVLF
                     AFDVAALATIAALAAAFTRYLLVSWWPAVFVLPFTPSAMKRRAMGTAARAWPWNPAAP
                     EPLGRTSGWTPALAAAALTAWLTAWHHVLIAAMGFRPADFREHLSLLAALYLALSLAL
                     VMPLHHSLARRAARGRRLWAPVAVAGLAAALVVAARALDWGTFFFSAGHVDEDFWAHA
                     FYGQVLGLGADPTVRLLAGAALLSTALAGLLLWRAVAFARRSRAWSLGPGGQTVVGAV
                     LRANAAWAAAGVLAASAAWPVIQAPSGRDLSDSVREAFAGVPEYKVAASLLRTLAPRL
                     PARMPAIDEAFAAKLERAGIRLGSIDPAWPLMKPSIYLDPASASAVGRPRVPAGTNLI
                     VILGESLSAALVDEAVHGIPGLTPAIDDFRRSAFTFRSLYSADFPTIKGELATLASFA
                     FDHRGLPITADSGNPLKSRYLFLSDVLGEHGYSSYHLQSDFASFAGTASILGRHGYQR
                     IYAAEDPELRRRARHPIQKTWGLYDEDLFTAVADLIDEGQFRQPFLLTVATTDLHFPY
                     AALRRHPGTGGSALLDAVHSGDAAFGSFWERFKRSSRARDTLVLLTADHALVRRHFTR
                     GGAVPTRSQFDYVLASLYVPGDPAWAGGGTDTTCTQLDLAPTLLDVLGLDTPNPFLGL
                     SIFSERPRFPLALAREIPWERWTAAERAAVQAIGWTDADHRRWLDLLRHLAVTDRVMP
                     PGRPAAARPRSRRSRAPSARASR"
     gene            complement(4496..5476)
                     /locus_tag="PKK95_10160"
     CDS             complement(4496..5476)
                     /locus_tag="PKK95_10160"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOC18624.1"
                     /translation="MRHRRLVGSLGLAVGVALLVVMIVEAGPARLLAGALRVGPSFLL
                     VLAVSFSWFALNTWGWRLAFAAQDPEPPPGAWPLFGAYLAAEAVNNVTPLMALGGEPL
                     KVALLSPRVAAERSVAAVVGDNVVHAITAPLFMLGGLALGWAAFDLERGLWLRLLAAT
                     AAIGLLALAAWLAACRGAVGGLVRLVARRFREGAGATLGAKADRVDALTSSFLGGGWR
                     FWASIALHLAGRTMGAVEAWIIVTALGVPFSLAGAVFVIAVAHVAVNLAFSIVPSQLG
                     VQEAAAYLLFAAVGLDPASGVTLMLVRRVRGFVWIAVGLLLAGRGASPGR"
     gene            complement(5473..7035)
                     /locus_tag="PKK95_10165"
     CDS             complement(5473..7035)
                     /locus_tag="PKK95_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18625.1"
                     /translation="MRIIPRFWTLALALLIVLRAPALAIDVLDPDEAGHAVNAATWLE
                     GGVPYVDFADNKQPFVYGAYALAFAFCGRSLAAVHALTLPWLLATGWLVGGLAAATWG
                     TTVARRWGVLLFVLGGSAYLEKDMLATNTEVLMNLPLAAAFWVVFAPSRLPAGVVAAL
                     SGLAAGAALLFNLKAALALPGLLLALASFGGAARWARAAIWTASAGVLPAVVVTYLWH
                     RGGLADAWFWNVALNFSYSGAGLPLGSLGVAGGTLYGFPRLLLFGLATLPLWIGAGVA
                     ARRAWPDRERRASAAAAFVWLAGSLAGACIGGRFYGHYFVALMPPLAWLASGPLASVL
                     ARPAGSQRAPRAWLRAAAVLGLVVPVAGLTIAGWVRISNRHLDALRPEVFEVADAVRQ
                     RTSPGDRVFVWGYWPQLYYYAGRPPATRFVFPQTLAGYVPGRPDVLASRPRDEQYMRG
                     DHWELWAADMARHPAELLIDTAPAAIHYWEHFPMSAYPPLADLVARRYRHEATVAGVS
                     IYRLRRDAGDSR"
     gene            7094..>7714
                     /locus_tag="PKK95_10170"
     CDS             7094..>7714
                     /locus_tag="PKK95_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18626.1"
                     /translation="MIRVAAIVLPSCSPAPSDWFLARLAGLSHRDRLIATLRRAGIDD
                     VRIVEAGHVAGDVPRACRPLLVVDGRRLYAPSWIEAAVGGAGPGDFAAGGRPSGLRVA
                     PEGAALDGAWTDFERVVPGAAQLSVPAGAWSVEVQTPQGRHRAREQLRLSLAKPTDGW
                     FARRFNRPISTRLSIRLASLGVHPDLATAAALALAVAAAWSSALGTW"
BASE COUNT         1043 a         2879 c         2772 g         1020 t
ORIGIN      
        1 tggggacgcg cttcccgttc tgcaactacc agtcgggcaa gatctggggt acgcccgccg
       61 acgagcccgg cgccgcacct gacccccgcc aggtggttga agcgtcgtgg cccatgctgc
      121 tctcggatct cgagctccgc gcgccggcgt tcgtgctcga cgcggcggcc gccgggctcg
      181 accggtgggc ggggcacgag atcgcccgct acccgcgtct cgcctccgcc gtccaggaac
      241 gttaccggct cgtcgccgtc gtctccggcg tggcgatcta ccagcgccgc gagtccgtcg
      301 agacttcctc ggtgaggcac ccgtgatccg ccgcgtctcg caggcggtcg tggtgctggc
      361 cgacggggtg ggtgcctcgt tcctccagcc gctgatcggg acgggcgagt acccggcgct
      421 cgaccgtctc gcggcggtag gcggacccgg cggcccgcgc gccgcctcca gcgtcttccc
      481 caccaccacg gggcccgcgc acctgccgtt catcaccggc cggtttcccg gcccgtgcaa
      541 catcccgggc atccgctggt tcgacccggg acgctacggc cggcacgcct tcggctcggg
      601 ccgtttccgc agctacatgg gcccgggcag ctacctggcc tcccgcgatc tcgcgcgcga
      661 ggtcgccacg ctgttcgagc tggtcgccga ccacgcctgc atcggcggcc acgcgcgccg
      721 gggcgtcggt cccggccgca acctgacgcg ctggagcaag gccgccacct cggtgctctc
      781 gttcgtgagc gaggactgga cgaggtcgga ccggatcgcg gcccgcaggc tggtggaggc
      841 cgtagaccgc ggcacacggc tcgtgttcgc gagcttctac ggcggcgacg cggcggcgca
      901 caagtggggg aaggggccgc aggtacggga agggtgccgg cgtatcgatt cggcgatcgg
      961 cgaggtgctc tcgcgcctcg agtcgaacgg gcgcgcggac cagaccctgc tgctggtggt
     1021 ctccgaccac ggcttctccg acaccaccgc ccacctcgac ctgcaccgcc tcgtgcagga
     1081 cgcgatcggc ccgaccctcg ctcaccccca ggtctggcgc ggctacttcg gcggcgccgc
     1141 cgcggcggtg atggtgtcgg gcaacgcgat ggcgcacgtc tacctgcgcg gcgccggctg
     1201 ggagcgttcg cccgcgctcg atccgcccgg accggaagcg tcgcggctgc tcgacgcgct
     1261 gctgcgggag ccggcggtgg accaggtgat cctgcgggga tcgcccactg cggcgatcgt
     1321 cgcgtcggcc cgtggccggg cccgcatctg ccgacacgag ggcaggatcc tgtacgctcc
     1381 ggaggcaggc cgagacgcgt tcggctacga cgcggggctt gccgggtgcc gcgacgagga
     1441 cggctggctg accgcgacgt gggacaccga ctatcccgac gcgcccgtcc agatcctcca
     1501 gctgctcgcg tccccgcggg cggggcacct gctcgtcacg gccaggcctg gcttcgacct
     1561 gcgagcgcgg ttcgagaagc cgccacaccg tggctcgcac ggggcgctgc accgactgca
     1621 cgtgatgacg ccgctgctct cgacccaccc actccgaagc ggcccggtgc gcacggcgga
     1681 cgtgatgccc accgtgctgg acgcgcttgg agagcccgtg ccgccggggc tcgacgggcg
     1741 atcgctgtgg cccgacagcc ggggagacgg cgcgtgaccc ggcgcatccg ccacgcgcgc
     1801 tcggcacggg tgctcgcggc cgcctgcacg gtgtcgggca tcgtgtgcct ggccgccgcg
     1861 ctggtcgcgg cggctggcgg ggcgagcggg gacacggcgc ccaggcatgc cgccgacgag
     1921 tggatcgacc ccgcctacaa cgtggtgatg cctccgggcg gcatcgtcgc cctcgacgcg
     1981 tcggccgatc ggttcgccgt cgaggtgttg aggcgcgagc gctcgagcac tgtgacgtcg
     2041 gtccatctcg cgctcacgat ccgggggcgc ggtgcagtcg tcggtggcgg cgagcaccgc
     2101 gcagcgatcg ccgatggccg gcatgcgctc gtatccgtgc ccgcgccaat gtcgccctgg
     2161 acgatcctcg cgaacggccg cctggccgcg ttcgtccggg cgtgggagta cggcggcttc
     2221 cagatcgacg ccgaggtgcg cgagggtggg cccggcggac gtctcgtgta ccggctgcac
     2281 gatccggccg cgctggttcg gatctcgcgg cgcggcttct tcgaccgcta cggcctcacc
     2341 ctctgcctgg cgggcctcgc cgccgcgttg ctcgcctcgt acgcctcggc gcgtctgctc
     2401 gggggaaccg ggtggcgcgt actcttcgcc ttcgacgtcg cggcgctcgc caccatcgcg
     2461 gccctggcag cggcgttcac ccggtatctc ctcgtgtcgt ggtggccggc cgtcttcgtg
     2521 ctgccgttca cgccgtcggc catgaagcgg cgtgcgatgg gcacggcggc gcgagcctgg
     2581 ccgtggaacc cggcggcgcc cgagcccctc ggcaggacgt cgggatggac gcccgcgctc
     2641 gccgccgcgg ccctgacagc atggctcacc gcctggcatc acgtcctgat cgcggcgatg
     2701 gggtttcggc ccgccgactt ccgcgagcac ctgagcctgc tggcggcgct gtacctcgcg
     2761 ctctcgctgg cgctcgtcat gccgctgcac cactcgctgg cccggcgggc ggcgcgcggc
     2821 cgccggctgt gggcgccggt cgccgtggcc ggtcttgccg cggcgctggt cgtggcggcc
     2881 agggccctcg attgggggac cttcttcttc tcggcgggcc acgtggacga ggacttctgg
     2941 gcgcacgcct tctacgggca ggtgctcggc ctgggcgcgg acccgacggt caggctgctg
     3001 gccggcgcgg cgctgctctc gaccgcgctc gcggggctgc tgctgtggag ggcggttgcg
     3061 ttcgcgcgga ggtcgcgcgc ctggtcgctc ggccccggcg gccagacggt ggtcggcgcc
     3121 gtgttgcgcg ccaacgcggc ctgggcggca gccggcgtcc tcgccgcgtc ggcggcgtgg
     3181 ccggtgatcc aggccccgtc cgggcgcgac ctcagcgaca gcgtccgcga ggcgtttgcc
     3241 ggcgtccccg agtacaaggt ggccgcctcg ctgctccgca cgctggcgcc ccggctgcct
     3301 gcgcggatgc cggcgatcga cgaggcgttc gcggcgaagc tggagcgggc cgggatcagg
     3361 ctcggctcga tcgatcccgc ctggcccctg atgaagccga gcatctacct cgatcccgcg
     3421 agcgcctcag ctgtgggccg gccgcgagtg ccggccggga ccaacctgat cgtgatcctg
     3481 ggcgagtcgc tctcggcggc gctggtggac gaggcggtgc acggcatccc gggcctcaca
     3541 cccgccatcg acgactttcg gcgatcggca ttcaccttcc gcagcctgta cagcgccgac
     3601 ttcccgacga tcaaggggga gctggccacc ctggcctcgt tcgccttcga tcacaggggg
     3661 ctgccgatca ccgccgacag cggcaacccg ttgaagtcgc gctacctgtt cctctcggac
     3721 gtgctcggcg agcacggcta ctcgtcatac cacctgcagt ccgacttcgc gagctttgcc
     3781 ggtaccgcga gcatcctcgg ccgccacggc taccagcgaa tctacgcggc cgaggacccc
     3841 gagctgcggc ggcgcgcccg gcatccgatc cagaagacct gggggctgta cgacgaggac
     3901 cttttcaccg ccgtcgcgga cctcatcgac gaagggcagt ttcgccagcc gttcctgctg
     3961 accgtggcga cgaccgacct gcactttccg tacgccgccc tccgccggca cccgggcacg
     4021 ggcggcagcg cactgctcga cgcggtgcac tccggggacg ccgcgttcgg atcgttctgg
     4081 gaacggttca agcgatccag ccgggcgcgc gacacgctcg tcctcctcac ggccgatcac
     4141 gccctggtgc gacggcactt cacgaggggc ggcgccgtgc cgacgcgctc ccagttcgac
     4201 tacgtcctcg cttcgctgta cgtccccgga gatccggcgt gggcgggagg cggcaccgac
     4261 accacctgca cgcagctcga cctggctccg accctgctcg acgtgctcgg cctcgacacg
     4321 ccgaacccgt tcctcggcct gtcgatcttc tcggagcggc cccgctttcc gctggccctg
     4381 gcgcgagaga tcccctggga gcgctggacg gccgccgagc gcgcggccgt gcaggccatc
     4441 ggctggaccg atgccgacca ccggcgctgg ctcgatctgc tgcggcacct cgcggtcacc
     4501 gaccgggtga tgccccccgg ccggccagca gcagcccgac cgcgatccag acgaagccgc
     4561 gcaccctccg cacgagcatc aaggtgacgc ccgacgcggg gtcgagcccc accgcggcga
     4621 acagcaggta ggcggcggcc tcctgcacgc cgagctggct ggggacgatg ctgaaggcga
     4681 gattgacggc gacgtgcgcg accgcgatga cgaagacggc gccggccagg gagaacggca
     4741 caccgagagc cgtgacgatg atccaggcct cgacggcgcc catggtccgg cccgcgaggt
     4801 gcagcgcgat cgaggcccag aagcgccagc cgccgccgag gaacgacgag gtcagcgcgt
     4861 ccacgcggtc tgccttcgcg ccaagcgtcg cgccggcccc ttcccggaac cttcgggcga
     4921 cgagccggac gaggcccccc accgcacccc ggcaggcggc cagccacgcc gccagggcga
     4981 gcagcccgat cgccgcggtg gcggcgagca gccgcagcca caaccctctc tcgaggtcga
     5041 aggccgccca tcccaaggcg aggcccccga gcatgaacag cggcgcggtg atcgcgtgca
     5101 cgacgttgtc gccgacgacc gcggccacgc tgcgctcggc cgcgacgcgg ggcgagagca
     5161 gcgccacctt cagcggctcc ccgcccagcg ccatcagcgg cgtgacgttg ttcaccgcct
     5221 cggcggccag gtaggcgccg aacagcggcc aggctccagg cgggggctcg gggtcttgcg
     5281 cggcgaatgc cagccgccat ccccatgtgt tcaacgcgaa ccaggagaac gagacggcga
     5341 gcaccagcag gaaggacggg ccgacgcgaa gcgctcccgc cagcagtctc gcggggccgg
     5401 cctcgacgat catcaccacc aggagcgcga cgccaaccgc aagcccgagc gacccgacga
     5461 gtcggcggtg cctcaccgcg agtccccggc gtcccggcgc agccggtaga tcgacacgcc
     5521 ggccaccgtg gcctcgtgcc ggtagcgacg ggccaccagg tcggccagcg gcgggtaggc
     5581 cgacatcggg aagtgctccc agtagtgaat ggcggctggc gccgtgtcga tcagcaactc
     5641 tgccggatgg cgcgccatgt ccgccgccca cagctcccag tgatcgcccc gcatgtactg
     5701 ttcgtcgcgg ggacgagagg cgaggacgtc ggggcggccc gggacgtaac cggccagcgt
     5761 ctgcggaaag acgaagcgcg ttgccggcgg ccgcccggcg tagtagtaca gctgcggcca
     5821 gtagccccac acgaacaccc ggtcgccggg cgaggtccgc tgccgcacgg cgtcagccac
     5881 ctcgaacacc tccggccgca gggcgtcgag gtgcctgttg gagatgcgaa cccaccccgc
     5941 gatcgtcagc cccgccaccg ggacgaccag cccgagcacc gccgccgcgc gaagccatgc
     6001 ccgcggggcg cgctggctgc cggccggcct cgcgagcacg gaagccagcg ggccggaggc
     6061 aagccacgcc agtgggggca tcagcgcaac gaagtagtga ccgtagaagc ggcccccgat
     6121 acaggcaccg gccaggctgc cagccagcca gacgaacgcg gcggcggccg acgcccggcg
     6181 ctcgcggtcg ggccacgcgc ggcgcgcagc cacgcccgcg ccaatccaga gcggcagcgt
     6241 cgccagaccg aagagcaaca gccgcgggaa cccgtagagc gtcccgcccg ccaccccgag
     6301 cgacccaagc ggcaggcccg cgccggaata ggagaagttg agcgcgacgt tccagaacca
     6361 ggcgtcggcc agcccacccc tgtgccagag ataggtgacg accactgccg gcaacacacc
     6421 cgccgacgcg gtccagatcg cggcgcgggc ccaacgcgcc gcgccgccga acgaggcgag
     6481 cgcgagcagc agccctggaa gtgccagggc agccttcagg ttgaacagca gcgcggcgcc
     6541 cgcggccagg cccgacagag ccgcgacgac gccggccggc agccggctgg gggcgaagac
     6601 gacccagaag gcggccgcca gcgggaggtt catcagcacc tccgtgttgg tggccagcat
     6661 gtccttctcg agatacgcag agccgccgag gacgaagagc agcactcccc accgccgcgc
     6721 cacggtcgtc ccccacgtgg ctgccgcaag cccgccgacg agccacccgg tggccagcag
     6781 ccacggcagc gtgagcgcgt gaacggcggc gagcgaacga ccgcagaagg cgaaggcgag
     6841 cgcgtaagcg ccgtagacga acggctgctt gttgtcggcg aaatcgacgt agggaacgcc
     6901 gccctcgagc cacgtggccg cgttgacggc gtgcccggcc tcgtcggggt cgaggacgtc
     6961 gatcgcgaga gccggggccc gcaacacgat gagaagcgcg agcgccaggg tccagaaacg
     7021 aggtatgata cgcactctca ccgctgggtt tcgacccacg cgattattcc ataagcgaac
     7081 tggattcacg gccttgatcc gcgtcgccgc gatcgtcctt ccctcgtgct cccccgcgcc
     7141 gtccgattgg ttcctcgcgc gcctggcggg gctttcgcac cgcgaccggt tgattgcgac
     7201 gctgcggcgg gcgggaatcg acgacgtgcg catcgtcgag gcgggccatg tcgcggggga
     7261 cgtcccccgg gcgtgtcggc ccctgctcgt cgtcgatggc cgacgccttt acgcgccctc
     7321 ctggatcgag gcggccgtcg gtggagcggg gcccggcgac ttcgctgcgg gcggccggcc
     7381 atcaggactg cgggtggcgc cggaaggtgc agcgctggac ggggcgtgga ccgacttcga
     7441 gcgggtggtg ccgggagcgg cgcagctgtc cgtccccgcc ggagcgtggt cggtggaggt
     7501 gcagacgccc cagggccgtc accgagcgcg cgagcagctg cggctctcgc tggccaagcc
     7561 gaccgacggc tggttcgccc gccgtttcaa ccgcccaatc tcgacccgcc tctccatccg
     7621 cctcgcgtcg ctcggcgtgc atcccgacct tgcgacggcg gccgccctcg cgctcgcggt
     7681 cgccgcggcg tggagctcgg ccctcgggac gtgg
//
LOCUS       DAOPQX010000173         7672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10647_length_7672_cov_4.496390, whole genome shotgun sequence.
ACCESSION   DAOPQX010000173 DAOPQX010000000
VERSION     DAOPQX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10647_length_7672_cov_4.496390"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKK95_10175"
     CDS             <1..361
                     /locus_tag="PKK95_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18627.1"
                     /translation="IDGKEADARSYLSALGVILYEAATGRRPFHGDSAASLAAAILEH
                     DPEPLGTLQPLAPPALEHVVSTCLAKDPEARWQSASDVARELRWIETAATRPSSTAIE
                     RPPHDRRTGREGYVVGE"
     gene            438..1031
                     /locus_tag="PKK95_10180"
     CDS             438..1031
                     /locus_tag="PKK95_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18628.1"
                     /translation="MTGAAAPEKLTESPGTFEYPAGWSPDGEWFVFTRLDNGTQYDLW
                     LLPARGDRKPVRFLETPFDENIASISPDGRWVAYDSDESGTPEIFVTSFPVPGEKHRV
                     STSGGYRAQWSPGGRQLFVWSSAGPFGSSLGPAYVVDVQTAPTFKAGTPRRLFTPPRN
                     VFGLSATRDLQRFLTAVPVEGWTPALRVTLNFQPAIE"
     gene            1217..2803
                     /locus_tag="PKK95_10185"
     CDS             1217..2803
                     /locus_tag="PKK95_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC18629.1"
                     /translation="MSKRAAFDSSVAVLTALAFGLGFTLVVPCGAAADGVGATPPVLV
                     TLWFDTEDYVLPQSDDAAKRLAEILTKLGVRATFKIVGEKARVLEQRGRIDVIQALRA
                     HEIGYHTDMHSRPPTPATYLQRAGWEDGIAEFLRREAAGAKDVERILGRPLTAYGQPG
                     ASWAPQAYPALRRMGIPMYLDEADHVGLDDQPFHFGGMLNVFRMRSTLARMELSGGTS
                     LEEGKAEFAAVADRVRAKGGGTVSVYYHPCEWVHERFWDAVNFSDGANPPREAWRKPG
                     VRPAAETEQAFADFERFIAFVKAQPGVRFVTASELAQLYADRALDREFTRDDLVSVAR
                     AVQGEQTFQRRDGYSLSVADMFSLLVQALATHAREGTLPASLRLRVLDGPAEAFEPAI
                     GSGQGQGDAAGGAAFAWEAFARATAEVARFTEDEKRVPAEVWVGARNVSPSEYLAMLG
                     AVVESLAATGRPPAEIARRSAAFTADRHVAAHAPGLWSWPIFPKGFHAPRIMQLARLQ
                     AWTLKPAVLAPGGGSRPQTS"
     gene            complement(2800..4026)
                     /locus_tag="PKK95_10190"
     CDS             complement(2800..4026)
                     /locus_tag="PKK95_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC18630.1"
                     /translation="MNTSQAQRYHAAATSFLALFSVVGLALYGLPLYYDHMVREFGWS
                     RTMVTSGNAISKLIVGPLFGFLAGWLIDRFGPRRFMLGGILMAGAAVAGLGSMTAAWM
                     FYAFYVLNALGNVCAGPLPNQVLLSRWFREGRGKAMGFAYLGIGIGGAIVPWLSYWLV
                     EAWGWRGALQALGAIIVLAAGPMIYFTKDPPAPERPEGEKKGSLPPLGPVLRRPAFYL
                     LLAGSMCSIAAVGGTNQHLKLFLSLDQGYAQAEAARAISLVLAFSIAGRLLMGWLADR
                     APKKHVMILIYLLIAVSIPLLFFASSRAVTQTFAVVFGLGLGGEYLIIPLIAAELFGV
                     GVLGRVMGIIVTADGVAEAVSPMLIGYLRDGTGSYQVGFIALIAVALVGTTAIALLPR
                     PGARPQEEPLAPAPSS"
     gene            complement(4079..4855)
                     /locus_tag="PKK95_10195"
     CDS             complement(4079..4855)
                     /locus_tag="PKK95_10195"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOC18631.1"
                     /translation="MNSRRVALVTGGTRGIGLGIARRLAQEGFDLAVCGLRAAELVKQ
                     PVAALRALGADVLYVACDVGEREQRERLFSGIRERFGRLHVVVNNAGVAPAERRDILD
                     ATEESFERVLAVNLRGPYFLTQAAARWMIEQKRHDRAFAGCIVNVSSVSATFASTNRG
                     EYCVAKAGLAMATTLWAARLGEFDIPVYEVRPGVIRTDMTAGVTAKYDRLIANGLLVQ
                     PRWGEPDDVGRAVAMLARGDLAYSTGQVIAVDGGMGVQRL"
     gene            complement(4879..6030)
                     /locus_tag="PKK95_10200"
     CDS             complement(4879..6030)
                     /locus_tag="PKK95_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC18632.1"
                     /translation="MVTHRIGIVMNGVTGRMGTNQHLLRSIVAIREQGGVRLADGGAI
                     VPEPLLVGRNRAKLEAIAARAGGVPWTTDLDQALGDDRYTIYFDAQSTDRRAAAVRRA
                     IAAGRHVYCEKPSAATVAEALELYELARDAGVCHGVVQDKLWLPGIVALRELMESGYF
                     GRLLSVRGEFGYWVFEGDEVAPQRPSWNYRAEEGGGIIADMFAHWRYVLDEVFGRVVA
                     VSCLGATHIPQRWDEQGRPYACTADDAAYATFKLEGGVIAQFNSSWAVRVRRDDLLTI
                     QVDGTKGSAVAGLRRCWAQPAAATPRPVWNPDEDSAHDYRNDWREVPTARAPENAFKA
                     EWELFLRHVVCGDPFPWDLLAGARGVQLAELGYESWKAGRWVEVEELGA"
     gene            complement(6112..6546)
                     /locus_tag="PKK95_10205"
     CDS             complement(6112..6546)
                     /locus_tag="PKK95_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOC18633.1"
                     /translation="MNILDLDTVQGRRYPAGRVTKNVVGGTSPIQAEHFCAGVVVLDA
                     EGGQVPWHNQDQEEVYLVLEGAGEMCLGDERAILTAGQAVSIPPGVFHQITNVGLGPL
                     RLLYVYGPAGDVAHWRQELDGSLPRAGVDAPRLPEGARPQKA"
     gene            complement(6610..>7672)
                     /locus_tag="PKK95_10210"
     CDS             complement(6610..>7672)
                     /locus_tag="PKK95_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18634.1"
                     /translation="IVSTYGTLRRLMLEGRIPRNDWERRCYETALRCSAAVQAHRWTT
                     LGEREGFIHSFNGPHSLFVDTLRTLRALAVGHRLGQVLLEEGGARVSLLARLVRHARA
                     TSSYLVAEGKGRDVYDVRGRVTHEGIFNTRTGAFRCLSTQQGYSPFTTWSRGLAWAML
                     GFSEQLEFLATVPPRELADEGGKAAVEAFMRRAACDACDRYIEDAAADGIPYWDFGAP
                     GLRHLGDDRAKPADPFNDHEPVDSSAAAIAAQGLLRLGHYLRRREPGRARRYWQAGLT
                     VMRALTGAPYLSTSPRHEGLLLHSVYHRPNGWDAVPPGRRVPCGESSMWGDYHLREAA
                     LYVQRVIRRDPYPVFWGGS"
BASE COUNT         1172 a         2772 c         2638 g         1090 t
ORIGIN      
        1 aatcgatggg aaggaggccg acgcccgcag ctacctgtcc gcgctcgggg tgatcctcta
       61 cgaggcggcc accgggcgcc ggccgttcca cggcgacagc gcggccagcc tcgcggccgc
      121 aatcctcgag cacgaccccg aaccgctcgg cacgctgcag ccgctcgctc cgcccgcact
      181 cgagcacgtc gtctcgacct gcctggccaa ggaccccgag gcgcggtggc aatcggcaag
      241 cgatgtcgcg cgcgagctgc gctggatcga gaccgcagcg acaaggccgt cttcgaccgc
      301 gatcgaacgg ccgcctcacg accgacggac gggccgcgaa ggttacgtcg tgggcgagtg
      361 acgtcaacct ggtctggtcg gcggacggaa accgcattgc ctacgtctgt ggaagcgacg
      421 acctctgtca gctccggttg accggggcgg ccgctccgga gaagctgacg gagtctcccg
      481 gcacgttcga gtacccggcc ggctggtcgc cggacggcga atggtttgtg ttcacgcgcc
      541 tcgacaacgg cacccagtac gacctgtggc tgctgccggc gcggggcgac cgcaaaccgg
      601 tgcggttcct ggagacgccg ttcgacgaga acatcgcctc catctctccg gacgggcgtt
      661 gggtcgccta tgactccgac gagtcgggca cgccggagat cttcgtgacc tcgttcccgg
      721 tgccgggcga gaagcaccgg gtctccactt cgggcgggta ccgcgcgcag tggtcacccg
      781 gcggcaggca gctgttcgtc tggtcgtcgg cagggccgtt cggctcgtcg ctcggcccgg
      841 cctacgtcgt tgacgtgcag acggccccga ctttcaaggc gggcacgccc cgtcgtcttt
      901 tcactccgcc gcggaacgtc ttcggccttt ctgccacccg cgacctgcag cgcttcctga
      961 ctgccgttcc cgtggagggg tggacgccag cgctccgagt cacgctgaac ttccagcccg
     1021 cgatcgaatg atcggtcggc taccgggaag ctggttcgcg ggtcgcgccg cgggacttgg
     1081 gtcgcgcagc ggggcacccc cgcgctgcac ttgcgtttgc cttaccagcc ggtgtctcat
     1141 tgcagcgatg acgtcacctc aggcctgctc gcgcgatgcc cagctcgcgc tgtgagcggg
     1201 tgacccggga gcgaccatgt ccaaacgtgc ggcctttgac agtagcgtcg cggtgctgac
     1261 cgcgctcgcg ttcggcctcg gtttcaccct cgttgtcccc tgtggagcgg cagccgacgg
     1321 ggtcggggcg acgcccccgg tcctggtcac gctctggttc gacaccgagg actacgtgct
     1381 cccgcagtcg gacgatgcgg cgaagaggct ggcggagatc ctgacgaagc tgggagttcg
     1441 ggccacgttc aagatcgtgg gcgagaaggc gcgcgtgctc gagcagcgcg ggcgaatcga
     1501 cgtgatccag gcgctgcgcg cccacgagat cggctaccac accgacatgc acagccggcc
     1561 cccgacgccg gcaacctacc tgcagcgcgc gggatgggag gacggcatcg cggagttcct
     1621 gcggcgcgag gcagcggggg cgaaggacgt cgagcgaatc ctcggccggc cgctgaccgc
     1681 gtacgggcag ccgggcgcct cgtgggcccc gcaggcgtat cccgcgctcc gcaggatggg
     1741 aatcccgatg tacctggatg aggccgacca cgtcggcctc gacgaccagc cgttccattt
     1801 cggcgggatg ctgaacgtgt tccgcatgcg atcgacgctg gcgcggatgg agctgagcgg
     1861 aggtacgagc ctcgaggagg gcaaggccga gttcgccgcg gtggccgatc gcgtgcgcgc
     1921 aaagggcggc ggaaccgtca gcgtctacta ccacccttgc gagtgggtgc acgagaggtt
     1981 ctgggacgcg gtgaacttca gtgacggagc caacccgccc cgtgaggcgt ggaggaaacc
     2041 cggcgtgcgg ccggccgccg agaccgagca ggcgttcgcc gacttcgagc ggttcatcgc
     2101 gttcgtgaag gcgcagccgg gcgtgcgatt cgtcaccgcg tccgagctgg cgcagctgta
     2161 cgcggaccgg gcgctcgacc gcgagttcac gcgcgacgac ctcgtgagcg tggcgcgtgc
     2221 cgtccagggc gagcagacgt tccagcggcg tgacgggtac tcgctgtcgg ttgccgacat
     2281 gttctctctg ctggtgcagg cgctcgcgac gcacgccagg gaggggacgc ttcccgcgtc
     2341 gctccggctg cgcgtgttgg acgggccggc ggaagcgttc gaaccggcca tcggaagcgg
     2401 ccaggggcaa ggcgacgccg caggcggcgc ggcgttcgcc tgggaggcat tcgctcgcgc
     2461 cacggcggag gtggcccggt tcacggagga cgagaagcgc gtgccggccg aggtgtgggt
     2521 cggcgcccgg aacgtatccc cctccgagta cctcgcaatg cttggcgccg tggtggagag
     2581 ccttgccgcc acgggacggc cgccggcaga gatcgcgcgc cgctcggccg ccttcaccgc
     2641 cgaccggcac gtcgccgccc acgctccggg tctctggagc tggccgatct tccccaaggg
     2701 cttccacgcc ccgcgtatca tgcagctcgc ccgtctgcag gcgtggacgc tgaagccggc
     2761 tgtgctggcg ccagggggag gtagcaggcc gcagaccagc tagctgctgg gggctggggc
     2821 cagcggctct tcttgcggcc tggcccctgg gcgcggaagc agggcgatgg cggtcgtgcc
     2881 gacgagcgcc acggcgatga gcgcgatgaa gcccacctgg tagctgccgg tcccgtcgcg
     2941 caggtagccg atcagcatcg gcgacaccgc ctcggccacg ccgtcggcgg tgacgatgat
     3001 ccccatgacc cgccccagca cgcccacccc gaacagctcg gccgcgatga gcgggatgat
     3061 caagtactcg ccgccgaggc caaggccgaa gaccaccgcg aaggtctgcg tcacagcccg
     3121 cgacgaggcg aagaacagga gcgggatcga cacggcgatc agcaggtaga tcaggatcat
     3181 cacgtgcttc ttcggcgcgc ggtcggccag ccaccccatc agcaggcggc ccgcgatgct
     3241 gaaggcgagc acgagcgaga tggcccgcgc ggcctcggcc tgcgcgtaac cctggtcgag
     3301 gctcaggaac agcttcaggt gctggttggt cccgccaacg gcggcaatcg agcacatgct
     3361 cccggcaagc agcaggtaga acgccgggcg ccgcagcacc ggcccgagcg gcggcaggct
     3421 ccccttcttc tctccttccg gccgctccgg cgcgggcgga tctttcgtga agtagatcat
     3481 cgggccggcc gccagcacga tgattgcgcc cagcgcctgc agcgccccgc gccagcccca
     3541 cgcctcgacc agccagtacg acagccacgg cacgatcgcg ccgccgatgc cgatgccgag
     3601 gtacgcgaag cccatcgcct tgccgcgtcc ctcgcggaac caccgggaga gcagcacctg
     3661 gttcggcagg ggccccgcgc acacgttccc gagcgcgttg agcacgtaga aggcatagaa
     3721 catccaggcg gccgtcatgc tccccaggcc cgcgaccgcg gcgccggcca tcaagatgcc
     3781 gccgagcatg aaacgcctgg gcccgaagcg gtcgatgagc caccccgcaa ggaacccgaa
     3841 gagcggcccc acaatcagct tgctgatcgc gttgccggag gtgaccatcg tgcgcgacca
     3901 cccgaactcg cgcaccatgt ggtcgtagta gagcggcagg ccgtacagcg ccaggcccac
     3961 gaccgagaac aacgcgagaa aggaggtggc ggccgcgtgg tagcgttgag cctggctggt
     4021 gttcatggga ttgtcagacg tctatctccg cgtccccttc gcgcgccgct cacagagcct
     4081 aaagccgctg gactcccatc ccgccgtcca ccgcgatcac ctgccctgtc gagtacgcga
     4141 ggtcgccgcg cgccagcatc gccaccgccc ttcccacgtc gtcgggctcg ccccaccgcg
     4201 gctgcaccag cagaccgttc gcaatcagcc ggtcgtattt cgcggtgacg ccggccgtca
     4261 tgtcggtccg gatgacgccc ggccgcacct cgtagaccgg gatgtcgaac tcgccgagcc
     4321 tggccgccca gagggtcgtg gccatcgcaa ggcccgcctt cgccacgcag tactcgcccc
     4381 ggttggtcga agcgaaggtt gccgacaccg acgacacgtt gacgatgcag ccggcgaagg
     4441 cgcggtcgtg ccgtttctgc tcgatcatcc agcgggcggc ggcctgcgtc aggaagtacg
     4501 gcccgcgcag gttgacggcg agcacgcgtt cgaagctctc ctcggttgca tcgaggatgt
     4561 cgcggcgctc ggccggggca acgcccgcgt tgttgaccac gacgtgcagg cggccgaagc
     4621 gctcgcggat gccagagaac aagcgctcgc gctgctcgcg ctcgcccacg tcgcacgcga
     4681 cgtacaggac gtcggcgccg agcgcgcgca gggcggccac gggctgcttc accaactcgg
     4741 ccgcccgcaa gccgcacacc gccaggtcga agccttcctg cgccagccgc cgcgcgatgc
     4801 ccagaccgat cccccgcgtc ccccccgtca ccagcgccac gcgcctcgag ttcatcaccg
     4861 tatcgccctt ctactctctt acgcccccag ctcctccacc tccacccacc tccccgcctt
     4921 ccacgactcg tagcccaact cggcgagctg cacgccgcgg gccccggcga gcaggtccca
     4981 cgggaacggg tcgccgcaga cgacgtggcg gaggaacagc tcccactcgg ccttgaaggc
     5041 gttctcgggc gcgcgggccg tcggcacctc gcgccagtcg ttgcggtagt cgtgcgccga
     5101 atcctcgtcc gggttccaga ccgggcgagg cgttgccgct gccggctgcg cccagcagcg
     5161 ccggaggccg gccaccgccg agcccttcgt cccgtccacc tggatcgtga ggaggtcgtc
     5221 gcgccgcacg cgaacggccc acgacgagtt gaactgcgcg atgacgccgc cctccagctt
     5281 gaacgtcgcg taggccgcat cgtcggcggt gcaggcgtac gggcggccct gctcgtccca
     5341 acgctgcggg atgtgcgtcg cgcccaggca cgagacggcg acgacacgcc cgaacacctc
     5401 gtcgagcacg taccgccagt gggcgaacat gtcggcgatg atgccgccgc cctcctcggc
     5461 ccggtagttc caggacgggc gctgcggggc cacctcgtcg ccttcgaaca cccagtagcc
     5521 gaactccccg cgaaccgaca gcagccggcc gaaatacccg ctctccatca gctcgcgcag
     5581 cgccacgatg ccgggcagcc agagcttgtc ctgcacgacg ccgtggcaca ctcccgcgtc
     5641 gcgtgccagc tcgtacagct ccagggcctc ggccaccgtc gcggcgctcg gcttctcaca
     5701 gtagacgtgc cggccggcgg caatcgcgcg gcggaccgcg gcggcgcggc ggtcggtgga
     5761 ctgcgcgtcg aagtagatgg tgtagcggtc gtcgccgagc gcctggtcga ggtccgtcgt
     5821 ccagggaacg ccgcccgcgc gcgcggcgat ggcctcgagc ttcgcccggt tgcggccgac
     5881 cagcagcggc tcgggcacga ttgcgccgcc atcggcgagc cggacgccgc cctgctcgcg
     5941 gatggcgacg atcgagcgca gcaggtgctg gttggtcccc atgcgcccgg tcacgccgtt
     6001 catcacgatg ccgattcgat gtgtaaccac agttcgattc cctgagcaca gggcgaccac
     6061 aagggtcgcc ccctacgccg ccgtgtcata gggcgaccac aagggtcgcc cctacgcctt
     6121 ctgcggccgg gcgccctcgg gcagccgcgg cgcgtccacg ccggcgcgcg ggagcgaccc
     6181 gtccagctcc tggcgccagt gggcgacgtc tcccgccgga ccgtacacat acagcaggcg
     6241 cagcggcccc aggccaacgt tcgtgatctg gtggaagacg ccgggcggga ttgacaccgc
     6301 ctggccggcg gtgaggatcg cgcgctcgtc acccaggcac atctcgccgg cgccctccag
     6361 caccaggtag acctcctcct ggtcctggtt gtgccacggc acctggccac cctcggcgtc
     6421 gagcaccacc acgcccgcgc agaagtgctc ggcttggatc ggcgaggtac cgccaacgac
     6481 gttcttggtc acgcggccgg ccggatagcg ccgtccctgc acggtgtcga gatcgagaat
     6541 gttcatggca acctcacacc agccggctgc ggcaattgaa gccgtgatgt tccttgcttc
     6601 agcaacctgc tagctccctc cccagaacac cgggtacgga tcgcgccgga tgacgcgctg
     6661 cacgtacagg gcggcctcgc gcaggtggta gtcgccccac atgctcgact cgccgcaagg
     6721 cacgcggcgg cccggcggca ccgcgtccca cccgttcggc cgatggtaga cggagtgcag
     6781 caggagcccc tcgtggcgcg gcgaggtgga aaggtacggc gcgccggtga gcgcgcgcat
     6841 cacggtgagg cctgcctgcc agtaacggcg agcgcgcccc ggttcgcggc ggcggaggta
     6901 atgtcccaga cgcagcaatc cctgggcggc aatcgcggcg gccgaactgt ctacgggctc
     6961 gtggtcgttg aagggatcgg ccggcttcgc tcgatcgtcg cccaggtgcc gcaggccggg
     7021 cgcgccgaaa tcccagtacg ggatgccgtc ggcagcagcg tcctcgatgt agcggtcgca
     7081 cgcgtcgcac gccgcgcggc gcatgaacgc ctccaccgct gccttgccgc cctcgtctgc
     7141 cagctcgcgc gggggcacgg tggcgaggaa ctcgagctgt tccgagaagc cgagcatcgc
     7201 ccaggccagg ccgcgggacc aggtggtgaa cggcgagtag ccctgctgtg tgctgaggca
     7261 ccggaaggcg ccggtcctcg tgttgaagat cccctcgtgg gtcacccgcc cccgcacgtc
     7321 gtagacgtcg cgaccctttc cctcggcgac gaggtacgac gaggtcgcgc gggcgtgccg
     7381 caccaggcgt gccagcagcg agacgcgcgc cccgccctcc tccagcagca cctggcccag
     7441 gcgatgcccg acggccaacg cccgcagcgt gcgcagggtg tccacgaaca gcgaatgcgg
     7501 cccgttgaac gagtggatga agccttcgcg ctcgccgagc gtcgtccagc ggtgcgcctg
     7561 cacggccgcc gagcacctga gcgccgtctc gtagcagcgc cgctcccagt cgttgcgcgg
     7621 gatccgcccc tcgagcatca gccgccgcag cgtgccgtag gtgctgacga tg
//
LOCUS       DAOPQX010000174         7662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10668_length_7662_cov_3.788090, whole genome shotgun sequence.
ACCESSION   DAOPQX010000174 DAOPQX010000000
VERSION     DAOPQX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10668_length_7662_cov_3.788090"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKK95_10215"
     CDS             complement(<1..335)
                     /locus_tag="PKK95_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18635.1"
                     /translation="MPPLARRPAAHAALALTLLLAAVEPARAELVFFTSGRTLSVKAH
                     RIEGESIVLLLRSGGEIACSLSLVERIAPDELPYPEPEPDVIPPVPYRELVAAAAARE
                     GVDARLVDAV"
     gene            complement(351..1229)
                     /locus_tag="PKK95_10220"
     CDS             complement(351..1229)
                     /locus_tag="PKK95_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18636.1"
                     /translation="MKSFLPVALLCAALAATAGACRRSNDSNEPAVATPRVTLNHGRV
                     PLGSPLEITYEFKVAENARFDQDYLVFSHFLDADGELMWTDDHHPPTPTTQWKPGETI
                     KYQRLMFVPVYPYVGEAALDVGLYSRDGRRLRLEAADQGQRAYRVATLQVLPQTENIY
                     LTFKDGWHQGELANDNAGNEWHWTKKQASLAFKNPGRDVVLFLDVDGRSRLLATPQVV
                     TVAAGTETVATFQLGEASEVKQIPITAAQLGTDDTVLLTISVDQTFVPAVVTAGAQKD
                     TRELGIRVFHAFVEAR"
     gene            1528..3279
                     /locus_tag="PKK95_10225"
     CDS             1528..3279
                     /locus_tag="PKK95_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOC18637.1"
                     /translation="MISRSMRLASATAIAAGVLAGCGSGLQTKPPAVAPAVPAEASAA
                     APVAQLPAEDPVLELIAVSQRHFETGQQELSIGHLDRARGEFNKALDVLLRSPYGART
                     EPRLREHFDRLVDRISTYEVTALAQGDGFSEQASEPASIDALLELATQVPDAAPSAAL
                     SRLVASDLVATAHDIEIPLNARVLAYVELFQGKLRDWIASGLERGARYLPMIQDVFRA
                     EGLPLDLAYVPLIESAFKPNAVSRAKARGVWQFMAGTALAHGLKRDWYVDERSDPEKS
                     TRAAAKYLRSLVRTFDGDWHLALASYNGGPGRVQRALKRFNKDDFWSLAANPRALPRE
                     TREYVPMILAAIVIARNPIEYGFAGVTPPPDTLHETVRVSRPVDLRRVAEWAGTSAET
                     IQELNPELRRWTTPVRSPEYELKVPSGTADLLKARLDAADSAEMAALQWYTVRRGETL
                     QSIARKLGVSRANLAEANYLSVRARVATGQKLIIPAAPTLLLASNADRPVPVAASRSV
                     VPADAVPATAPASPEGSDRIRVVYRVKRGDTLSSVARLFRTSVGAIKRWNGLRSDHLQ
                     TGAQLTVFAAARPQGKP"
     gene            3430..4971
                     /locus_tag="PKK95_10230"
     CDS             3430..4971
                     /locus_tag="PKK95_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOC18638.1"
                     /translation="MLASLRTAAVFGLDVFPVQVEVEVSFGLPSFKMVGLPDPSVRES
                     RDRVRSAIRNSGFDFPPMRVTVNLAPADVRKAGASFDLPIALGVLAATGVLARRQVHD
                     VVLVGELSLDGAIQATRGVLPVAVAARRDGVGSILLPAANAAEASVVEPLDVIPVSSL
                     AEAVRAINEPGSVSRPPRAVLRPPSPAWAVDLAEVRGQSLARRALEVAAAGGHNLLMV
                     GPPGAGKTMLARRIPGILPPLTFDESLESTAIHSVCGLLPPGCGLLADRPFRAPHHTI
                     SEVALVGGGSLPRPGEISLAHNGVLFLDEMPEFDRRVLEVLRQPLEEGYVTIARAART
                     ARFPARFMLVAAMNPCPCGYLGDLVRACRCTPVEVERYRGRLSGPLRDRIDLVLEVPA
                     VPAALLADDGSGEKSAAVRERVLTARERQAERRAETGGVRVNAALQGRAVWRHCRPDA
                     PGRRLLERAVERLGLSARAYHRVLKVARTIADLHGLEMPGEPEIAEALQYRVEDARVL
                     NPPGR"
     gene            5157..6068
                     /locus_tag="PKK95_10235"
     CDS             5157..6068
                     /locus_tag="PKK95_10235"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOC18639.1"
                     /translation="MPRRYTLLIVDPVTGVEHRFTIYARRLMVVAGLVLLLPILAGLA
                     IRWSTHAETAALRATAATLQAENESFRAMTRALTDQLDSVQSAVAQLSVESKLDPDSA
                     RALARLPAAVRSGAMGGTATSTPIARSVLAPTLASPDDTFGMLREVLGRLGSRLDLVR
                     HDVQRRAALAASTPSIWPAQGYLSAGFGRRLDPFHGELAHHSGIDISAPKGQPVYATA
                     AGTVTAAGWNGDYGNMVTIDHGFGLVTRYGHLQDYSVKPGDKVERMQPIGRIGATGRA
                     TGPHLHYELLVNGQMTNPLRLLAGSRR"
     gene            6177..>7662
                     /locus_tag="PKK95_10240"
     CDS             6177..>7662
                     /locus_tag="PKK95_10240"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18640.1"
                     /translation="MLGALLAKVIGTQNERELKRIAPLVAAVNALEDQTRALSDEQLR
                     AKTVEFRGRLAAGESLDDLLPEAFAVVREAGYRTLKMRHFDVQLIGGIVLHMGRIAEM
                     KTGEGKTLVATLPAYLNALEGKGVHVVTVNDYLARRDSEWMGRIYRFLGLTVGVVQHE
                     LTDAERQVAYGCDITYGTNNEFGFDYLRDNMKFDLAHYVQRGHHFAIVDEVDSILIDE
                     ARTPLIISGPAEESTELYYEVDRIIPRLKPGAVTRGDTKAEEREALEATGDYLVDEKH
                     KTVTLTESGMAKAEKMLASRLQPGGLYDPANMPLLHHVNQALRAHTLFHRDVDYMVKD
                     GQVVIVDEFTGRLMPGRRWSDGLHQAVEAKEKVKIERENQTLATITFQNYFRKYAKLS
                     GMTGTAETEAEEFAKIYKLDVMVIPPNKPLRRLEEPDLIYRTVPEKLDAIVADVVEKQ
                     ASGRPVLVGTISIEKSEKLSTMLKRRGIKHVVLNAKYHAQEAEIV"
BASE COUNT         1114 a         2735 c         2681 g         1132 t
ORIGIN      
        1 accgcgtcga ccagccgcgc atccacccct tcccgcgcgg cggcggcagc gaccagctcc
       61 cggtagggaa ccggcgggat gacgtcaggc tcgggctcgg ggtacggcag ctcgtcgggg
      121 gcgattcgct cgacgagcga gagggagcag gcgatttccc cccccgatcg cagcagcagc
      181 acgatcgact cgccctcgat ccggtgcgcc ttgaccgaca gcgtgcggcc ggaggtgaag
      241 aacacgagct cggcgcgtgc cggctcaacg gcggccagca gaagcgtcag cgcgagggcg
      301 gcgtgcgcgg cggggcgccg cgccagcggc ggcatggagc caactccggg ctagcgcgcc
      361 tccacgaagg cgtgaaacac gcggatcccc agttccctgg tgtctttctg cgcgccggcc
      421 gtcaccacgg ccggcacgaa ggtctggtcc accgaaatgg tcagcagcac cgtgtcgtcg
      481 gtgcccagct gcgcggcggt gatcgggatc tgcttcacct ccgaggcctc gccgagctgg
      541 aaggtggcga ccgtctcggt ccccgcggcc acggtcacca cctgcggggt ggcgagcagc
      601 cgcgaccggc cgtccacgtc gaggaagagc accacgtcgc ggccggggtt cttgaaggcg
      661 aggctggcct gcttcttggt ccagtgccac tcgttcccgg cgttgtcgtt tgccaactcc
      721 ccctggtgcc agccgtcctt gaaggtgagg tagatgttct cggtctgcgg cagcacctgc
      781 agggtggcaa cgcggtaggc tcgctggccc tggtcggcgg cttcgaggcg gagccggcgg
      841 ccgtcgcgcg agtagagccc cacgtccagc gccgcctcgc ccacgtaggg gtagacgggc
      901 acgaacatca ggcgttggta cttgatcgtc tcacccggct tccactgggt ggtcggggtg
      961 ggcgggtggt ggtcgtcggt ccacatcagc tcgccgtcag cgtccaggaa gtgggagaag
     1021 acgaggtagt cctgatcgaa ccgcgcgttc tcggccacct tgaactcgta ggtgatctcg
     1081 agcgggctgc cgagcgggac gcggccgtga ttcagggtga cgcggggggt ggcgacggcc
     1141 ggctcgttgc tatcgttcga gcggcggcac gcgccggccg tggcggccag agccgcacac
     1201 agcagggcca caggaaggaa gctcttcata gaacaaagaa acccgggatg gcgccggtgc
     1261 tggaggttga gtatagcgta ctgctggggg cggcgccata ttgttgaggt tgtggcactt
     1321 acaagccggg ccattgaccg tcgcttcttg gccgtgctat cgtccgagct gagcggctct
     1381 ttctcgggcc gccccggcgg cgccgtgcgg cgtcgcgccg cccggccccg cggtccgggc
     1441 ggtcggaggc gtgctcccgc acgcccggcg aaactctcgt ccagccggct ggccccccag
     1501 gcccggctgg acctgcaggg agtgcgaatg atttcacgat cgatgcgcct cgcttccgca
     1561 acggcgatcg ccgcgggcgt cctcgccggc tgcgggtcgg ggctgcagac gaagcctccc
     1621 gccgtcgccc ccgccgttcc cgccgaggcc tccgccgcgg ccccagtggc gcagctgccc
     1681 gccgaagacc ccgtcctcga gctgatcgcc gtgtcgcagc ggcacttcga gacgggtcag
     1741 caggagctga gcatcgggca cctcgaccgg gcccgaggag agttcaacaa ggcgctcgac
     1801 gttctgctgc gttcccccta cggcgcccgg accgagccac gcctccggga gcatttcgac
     1861 cgcctggtcg accggatcag cacctacgag gtgaccgcgc tcgcccaggg cgatgggttc
     1921 agcgagcagg cctccgagcc ggcctcgatt gacgccctgc tggagctggc gacgcaggtt
     1981 cccgacgccg ccccgtcggc ggccctcagc cgcctggtcg cctccgacct cgtggcgacc
     2041 gcgcacgaca tcgagatccc gctgaacgcc cgcgtgctcg cgtatgtcga gttgttccag
     2101 gggaagctgc gcgactggat cgccagcggc ctggagcgcg gggcgcgcta cctcccgatg
     2161 atccaggatg tcttccgcgc cgaggggctg cccctcgacc tcgcctacgt ccccctcatc
     2221 gaaagcgcct tcaagcccaa cgccgtctcg agggcgaagg cgcggggcgt gtggcagttc
     2281 atggccggaa cggcgctggc ccacggcctg aagcgcgact ggtacgtgga cgaacgctcg
     2341 gaccccgaga agtcgacgcg agccgccgcc aagtacctcc ggtcgctcgt ccgcaccttc
     2401 gacggcgact ggcacctggc gctggcgtcg tacaacggcg ggccgggccg ggtgcagcgg
     2461 gcgctgaagc ggttcaacaa ggacgatttc tggtcgctcg ccgcaaatcc cagggcgctg
     2521 ccgcgcgaga cgcgggaata cgtcccgatg atcctcgcgg cgatcgtcat cgcacgcaac
     2581 ccgatcgagt acggctttgc cggcgtgacg ccgccccctg acaccctgca cgagacggtg
     2641 cgcgtctcgc gtcccgtcga tcttcgccgc gtcgccgagt gggcgggaac ctccgccgag
     2701 accatccagg agctgaaccc cgaactgcgg cgctggacga cgccggtcag gagcccggaa
     2761 tacgagctga aggtgccgtc agggaccgcg gatctcctca aggcgcggct cgatgccgcc
     2821 gattccgccg agatggccgc gctgcagtgg tacaccgtcc ggcggggcga gacgctgcag
     2881 agcatcgccc gcaagctcgg cgtcagccgc gccaacctgg ccgaggccaa ctacctctcg
     2941 gtgcgggccc gggtcgcgac cggccagaag ctcatcatcc ctgcggcccc gacgctgctg
     3001 ctggcctcca atgccgaccg cccggtgccg gtggcggcgt cccgctcggt cgtgccggcc
     3061 gacgccgttc cggcgacggc acccgcctcg cccgagggtt cggatcggat tcgcgtcgtc
     3121 taccgggtga agcgcgggga caccctgtcg tcggtcgcgc gcctcttccg cacgagcgtc
     3181 ggggcgatca agcgctggaa cggccttcgc tcggaccatc tgcagacggg ggcgcagctg
     3241 acggtcttcg ccgccgcccg cccgcagggc aagccgtaga gtttgcgagg aggtcgcaga
     3301 tgttgcggcc tcctgcccca ccgccttcgc accttctgcc ttccctgccc gccttcgccc
     3361 cagatcccgc ttgaattctg cgcttccagg cggccgcgaa ccgggccgcg ccttgcacta
     3421 ccgggcagcg tgctcgccag cctccgcacc gccgcggtgt tcgggctcga cgtgttcccg
     3481 gtgcaggtgg aggtggaggt gtcgttcggc ctgccgtcct tcaagatggt cggcctcccc
     3541 gacccgagcg tccgcgagag ccgcgacagg gtccgcagcg cgatccgcaa ctccgggttc
     3601 gactttcctc ccatgcgcgt cacggtgaac ctggcgccgg ccgacgtgcg gaaggccggg
     3661 gcatccttcg acctgccgat tgcgctcggc gtgctggcgg caaccggcgt gctggctcgg
     3721 cggcaggtgc acgacgtcgt gctggtgggc gaactgtcgc tggacggcgc gatccaggcc
     3781 acgcgcggag tgcttccggt ggcggttgcg gcgcgccgcg acggggtggg cagcatcctc
     3841 ctgccggcgg ccaacgccgc cgaggcgtcg gtggtcgagc cgctcgacgt catcccggtc
     3901 tcgtcgctgg ccgaagccgt gcgggcgatc aacgagccgg gctcggtctc acggccgccc
     3961 cgcgccgttc tgcggccacc ctcgccggcg tgggcggtcg atctggccga ggtgcggggc
     4021 cagtcgctcg cccgccgggc cctcgaggtg gccgccgccg gcggccacaa cctcctcatg
     4081 gtggggccgc ccggcgccgg caagacgatg ctggcccggc gcatccccgg catcctgccg
     4141 ccgctcactt tcgacgagtc gctcgagagc actgccatcc actcggtctg cggcctgctg
     4201 ccgcccggct gcggcttgct ggccgaccgc cccttccgcg cgccgcacca caccatctcg
     4261 gaggtggcgc tggtcggcgg cggctcgctg ccgaggccgg gcgagatctc cctcgcgcac
     4321 aacggcgtgc tgttcctcga cgagatgccc gagttcgacc gccgcgtgct ggaggtactg
     4381 cgacagcccc tggaagaggg ctacgtcacc atcgcccgcg cagcgcggac ggcacgcttc
     4441 cccgcccgct tcatgctggt cgcggccatg aacccctgcc cctgcgggta tcttggcgac
     4501 ctggtccggg cctgccggtg cacgccggtc gaggtcgagc gctaccgggg gcgattgtct
     4561 gggccgctgc gcgaccgcat cgacctcgtg ctcgaggtcc ccgcggtgcc cgcggccttg
     4621 ctcgccgacg atggaagcgg cgagaagtcg gcggccgtgc gcgagcgggt gctgaccgcg
     4681 cgcgagcggc aggcggagcg gcgcgcggaa accggggggg tgcgcgtcaa cgctgccctg
     4741 cagggacgcg cggtgtggcg ccactgccgt ccggatgcgc ccggccgccg cctgctcgag
     4801 cgcgcggtcg agcggttggg gctgagcgca cgcgcgtacc accgcgtcct caaggtggcg
     4861 cgcaccatcg ccgacctgca cggcctggag atgccgggtg agcccgaaat cgccgaggcg
     4921 ctgcagtatc gcgtggagga tgcccgggtg ctcaacccgc ccggccgatg acccgtcccg
     4981 tgaccttgtc ggtttgcctg cccgccgggg cgttcgtctt ttcttcgcca ggccagattt
     5041 ttcccgttcc cggccgaggt ttccctgtga tagtgttcag cgttacgtct catgggcggc
     5101 tggaccggct gatccagccg cgccgccagg accccgagca aggagtgcga cagccggtgc
     5161 ctcgacgcta caccctgctg atcgtggacc ccgtgacggg cgtggagcat cgcttcacga
     5221 tctacgcccg gcgcctgatg gtcgtcgcgg gcctcgtcct cctcctgccc atcctcgcgg
     5281 ggctcgcaat ccgctggagc actcacgcgg aaaccgccgc gctgcgggcg acggcggcca
     5341 cgctccaggc cgagaacgag agcttccggg caatgacccg cgccctcacc gatcagctcg
     5401 acagcgtgca gtcggcggtg gcgcagctca gcgtcgagtc gaagctcgat cccgactcgg
     5461 cacgcgcgct ggcacggctg ccggcggcgg tgcggtcggg ggcgatgggc ggcaccgcca
     5521 cgagcacccc aattgcgcga tcggtcctgg cgccgacact cgcctcgccc gacgacacgt
     5581 tcggcatgct gcgcgaggtg ctgggacgcc tcggcagccg gctcgacctg gtacgccacg
     5641 acgtgcagcg gagggccgca cttgccgcct cgaccccgtc catctggccc gcgcaggggt
     5701 atctctcggc cggcttcggc cgccgcctcg acccgttcca cggcgagctg gcccaccact
     5761 ccggcatcga catctcggcg ccaaaggggc agccggtgta cgcgacggcg gcgggcacgg
     5821 tgacggcggc gggctggaac ggcgactacg gcaacatggt gacgatcgac cacggcttcg
     5881 gcctcgtcac ccggtatggc cacctgcagg actactcggt gaagccgggc gacaaggtcg
     5941 aacggatgca gccgatcggc cgcatcggcg ccaccggccg cgccaccggc ccgcacctgc
     6001 actacgagct gctggtgaac ggccagatga ccaacccgct ccgcctgctc gccggctccc
     6061 ggcggtaggc gcgcccgcgg catctttccc gcgggcgcgc ggagcgtcag ttgacgctcc
     6121 gccccccctc ccctacaatc ttgtgatacc cccgtctgtt tcgcagctct ccttacatgc
     6181 ttggcgctct cctcgctaag gtcatcggca cccagaacga acgcgaactg aagcggatcg
     6241 cgccgctggt ggccgccgtc aacgccctgg aggatcagac gcgcgccctg agcgacgagc
     6301 agctccgggc gaagaccgtc gagttccgcg ggcgcctcgc cgccggggag tcgctcgacg
     6361 acctcctgcc cgaggccttc gccgtcgtgc gcgaggcggg ctaccgcacg ctgaagatgc
     6421 ggcacttcga cgtccagctc atcggcggca tcgtgctgca catgggccgg atcgcggaga
     6481 tgaagaccgg cgagggcaag acgctggtcg ccaccctgcc cgcctacctg aacgccctcg
     6541 agggcaaggg cgtgcacgtg gtgacggtca acgactacct ggcccgccgc gactcggagt
     6601 ggatggggcg catctaccgc ttcctgggcc tcacggtcgg cgtcgtccag cacgagctga
     6661 cggatgccga gcggcaggtg gcgtacggct gcgacatcac ctacggcacg aacaacgagt
     6721 tcggcttcga ctacctccgc gacaacatga agttcgacct cgcgcactac gtgcagcggg
     6781 ggcaccactt cgcgattgtc gacgaggtgg acagcatcct catcgacgag gcccggacgc
     6841 cgctgatcat ctcggggccg gccgaggagt cgaccgagct gtattacgag gtcgatcgca
     6901 tcattccccg cctcaagccg ggagcggtga cgcggggcga caccaaggcg gaggagcgcg
     6961 aggcgctgga ggccaccggc gactacctgg tcgacgagaa gcacaagacc gtgacgctca
     7021 ccgagagcgg catggcgaag gccgagaaga tgctcgcctc gcggctccag ccgggcgggc
     7081 tctacgaccc ggcgaacatg ccgctgctcc accacgtgaa ccaggcgctg cgggcccaca
     7141 ccctgttcca ccgcgacgtg gactacatgg tgaaggacgg ccaggtggtg atcgtggacg
     7201 agttcacggg ccgcctgatg ccgggccggc ggtggagcga cgggctgcac caggcggtcg
     7261 aggccaagga gaaggtgaag atcgagcgcg agaaccagac gctcgccacc atcaccttcc
     7321 agaactactt ccggaagtac gcgaagctct cggggatgac gggcacggcc gagaccgagg
     7381 ccgaggagtt cgcgaagatc tacaagctcg acgtgatggt gatcccgccc aacaagccgc
     7441 tgcggcggct cgaggagccg gatctcatct accggaccgt gcccgagaag ctcgatgcga
     7501 tcgtcgccga cgtcgtcgag aagcaggcgt cgggccggcc ggtgctggtc ggcacgatct
     7561 cgatcgagaa gtccgagaag ctctcgacga tgctgaagcg ccgcggcatc aagcacgtgg
     7621 tgctcaatgc gaagtaccac gcgcaggaag ccgagatcgt cg
//
LOCUS       DAOPQX010000175         7604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10751_length_7604_cov_4.822493, whole genome shotgun sequence.
ACCESSION   DAOPQX010000175 DAOPQX010000000
VERSION     DAOPQX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10751_length_7604_cov_4.822493"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            524..931
                     /locus_tag="PKK95_10245"
     CDS             524..931
                     /locus_tag="PKK95_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOC18641.1"
                     /translation="MTWLFKEEPAHYGYDALERDGRTVWSGVKNPLAQKHLREVKKGD
                     RILYYHTGGEKAIVGDAAAATDAYPDPDDGSGKAFVVDVVPKRRWRRPVTLAEIKATP
                     AFAQFPLVRMPRLSVMPVGEKELRELEKLAAGG"
     gene            complement(943..3963)
                     /locus_tag="PKK95_10250"
     CDS             complement(943..3963)
                     /locus_tag="PKK95_10250"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC18642.1"
                     /translation="MGSDGRRLKGTRAAEFVLAAAGPVSLDALVSWDIVRPTELWGWL
                     HGGRDQGWIGEDAAAGPGHFVLLDVERQARVLDAAGPGDWAWLFGLADLIPTVLANGR
                     RAADQRRQDVASALFHHMVLAASPESFPGGSHAWAGIVVECVRRFRFGSRLTPEILDR
                     AIDASVACGNLEWQAILCGARGFESVRTARDMNRGGEYLERALEAARALDSRAVSFEV
                     HLQVAFGQILVGQALAAVATFERLLGDLPQEILPLPPELQPLLDPVPETGLAILASTY
                     GQVGQYGRALDLLHRLRSAGERVGRPHLVGLADCFLSGLHAGRREFEPARLYAESAVR
                     YWTGDPTGPYFLWHASISLAWVRMIEGQLAAAADVLETGQRARVASGLMFFGGSALFE
                     VLDRMDVEGVKPPAGLDVETELVRMLGWPDGYMRAVAHRIRARRLARGPLDEEVAARI
                     SAHHEQAVALLRESRACPSELARALADAAQWAECRGRQPEAERLHDESRRVFASIDLA
                     PASGNVLELATALLELGRLGVNAAAGDTGWGDLAARLCAVLGVERCAIVERGAEAPLV
                     VASRGGGPAWVEAVRSLLAARPPEEVETGAPLLDDGPRGTGQLLVVPFGSTRLPKSGW
                     AVFENRYSRPLVSASDRRLLDVLGAQLGVLAENLSIWRDLIEARRRLEQENRYYRQAP
                     SLPSAGGRIVGESPALRQTLELVGRVAPTVTSVLIEGETGVGKELIAREIHRLSPRRP
                     GPFIAVHIASFAPGLVASSLFGHERGAFTGATEQARGRFELADGGTLFLDEVGELTLE
                     DQVRLLRVLQEGTFERVGGTRPLHSDFRLVAATNRDLRSSVARGEFREDLFFRLSAFP
                     IVVPPLRERREEIPTLALYFMERANRTLARAFQGIGEADMARLVAYRWPGNVRELEHL
                     IERAAILSEPPRLKIPPLQDETGDGGAPEASLGTLEEAERQYFRRLIARTAGRVSGPS
                     GAAAIAGLRPSTFTWHVEKLGLRADLQRARAARG"
     gene            4201..4467
                     /locus_tag="PKK95_10255"
     CDS             4201..4467
                     /locus_tag="PKK95_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18643.1"
                     /translation="MTYRIETNQAASEVSLSFQGLLDSAALDAVRSSVTAARAQGAKA
                     VVLTLAPGTTVDRECLPGLRSIEGLAVKAASRFLATWLAQCGVN"
     gene            4505..5167
                     /locus_tag="PKK95_10260"
     CDS             4505..5167
                     /locus_tag="PKK95_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18644.1"
                     /translation="MKSKMIVVVTAFLGLAIVSSAQAQEVRLNVGVRGSFGVPFGEAR
                     GGTDLKDVTTRAIPVQVDVDYRIDEHWSAGVYAIYGPVAVADATRRQLAASGLRSIGG
                     HRQQRVGVQLTRTFRPAARVSPWVGMAAGYDWTRYAWAKLATGEETEIGVSGFETTLQ
                     GGASFKLTPRLAIGPFAAFDLGRYGQDIVWVQRAGNSSTGIPDKGLHQWVRFGVKMSV
                     GF"
     gene            5357..6388
                     /locus_tag="PKK95_10265"
     CDS             5357..6388
                     /locus_tag="PKK95_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC18645.1"
                     /translation="MPLNRRQFLTTASAALAATTLDPRSLLAQAAGQAPPETAFKLLR
                     RGVGIFTGQGGTIGWFVDREGALVIDSQFPATAKICLEGLQQRMQASGTGVSPVKQAQ
                     GGTGVSPVKQAQGGTGVSPASGRLIDFLVNTHHHGDHVAGNGVFQPAVRKILAHVNVP
                     KLQREASERRGPGAPGPSTAPVVANATFDKAWHEEIGREEMEARYYGPAHTSGDAVVI
                     LEKANVVHMGDLVFNRMHPFIDRPAGASIASWITLLDRVPADMQRDTTYIFGHGNPKF
                     GVTGTSADLKYMRDYLAALLDFVQAEMKRGQPRDAIVRITDPLKGFADHGPLVERTLG
                     PAYDELAGS"
     gene            6440..>7604
                     /locus_tag="PKK95_10270"
     CDS             6440..>7604
                     /locus_tag="PKK95_10270"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HOC18646.1"
                     /translation="MPSRIRLAIGLAIAIVTLPNLARLDGQEQYDRTTLIPAKTLAAI
                     ANEVSGAQALNHVIDIAGYERDRRASEYGEGLPREAEVVASIAREAGLSAVEILRPAG
                     EPRLQWDGEEGELWIEEPIASRRLLTRYRDVAATLAPNSLTTEVTADLVFVGRGDREA
                     DYEGKDVKGKIVLGWGSLGAIHGLAVTRYGAAGAISYGVSSGKPIDRPDQIAWGSIGT
                     FPPDGEKTGLSRASWFGFNLSHRMGLQLVDLLDKGTVRLRAKVRASQYPVQLPTVTAT
                     IPGDGSLAATGKDEVVFVAHLFEGATKQGANDNTSGVAVQLEIARAWIALVRSGALPA
                     PRRTIRFLWVPEISGTMAYLREHREEALRMLAAINMDMVGANQTLHRNSANLAY"
BASE COUNT         1229 a         2614 c         2701 g         1060 t
ORIGIN      
        1 gagccggcgg gcaagcgtga ggtcggagaa catgggatca gttaaacaca ggacggaagg
       61 cagcaatcgg gattcgggaa tcaggattca ggaatcagga ttcaggaatc aggattcggg
      121 aatcgagatt caggaatcga gagccggtaa tcaggaacag gagcggaagt gcggagcagg
      181 gagcggaact cagaaagcgc gggcgggggt gaggagcgcg ggggcgggac gctccgaggc
      241 ttacttcgcc ttcttcggcg ccgccttctt cacggtcttg gccttcgcga cgcccttctt
      301 cggtccgcag cacgccatga gaatcctcct ccgatcggcg cggcgccatg ccgcgcggac
      361 gcccgaattc taacctgaat cctgattccc gcatcccgta tcccgattcc tccactgacc
      421 ctgaggccgt accaaagacg ttgcgttctc cacgctttcg gtatgggtcc cccctctgct
      481 acgctggacc taggatttcc gggcaaaatc gagcggaggg gcgatgacct ggctcttcaa
      541 ggaagaaccg gcccactacg gatacgacgc gctcgagcgc gacggccgga cggtctggtc
      601 gggggtgaag aacccgctcg cgcagaagca cctgcgcgag gtgaagaagg gtgaccgcat
      661 cctctactac cacaccggcg gcgagaaggc gatcgtcggc gacgcggcgg cggccaccga
      721 cgcctacccc gaccctgacg atggttcggg caaggcgttc gtggtggacg tcgtcccgaa
      781 gcggcgatgg cggcgcccgg tgacgctggc cgagatcaag gcgacgcccg ccttcgccca
      841 gttccctctg gtgcggatgc cgcggctctc ggtgatgccg gtcggcgaga aggagttgcg
      901 ggagctggag aagctggcgg ctgggggctg aacggttagc agtcaccccc gcgcggcgcg
      961 ggcgcgctgc agatcggcgc gcaggcccag tttctcgacg tgccaggtga aggttgacgg
     1021 cctgagtccg gcgatcgccg cggcgcccga cggtcccgag acccgaccgg cggtcctcgc
     1081 gatgagccgc cggaagtact ggcgctccgc ctcttcgagc gtgccgaggg acgcctcggg
     1141 tgcaccgccg tcgcccgttt cgtcctgcag agggggaatc ttgagtcgcg gcggctccga
     1201 caggatcgcc gcccgctcga tgaggtgttc cagctcgcga acgttgccgg gccagcggta
     1261 cgcgaccagc ctcgccatgt ccgcctcgcc aatgccctgg aaggcacgcg ccagggtgcg
     1321 gttcgcccgc tccatgaaat agagcgcgag cgtggggatc tcctcgcgcc gctcgcgcag
     1381 cggcggaacg acgatgggaa atgccgacag gcggaagaag agatcctcgc ggaactcccc
     1441 gcgcgcgacc gaggacctca ggtcgcggtt ggtggccgca acgagccgga agtcggagtg
     1501 caacggcctg gtgcccccga cgcgctcgaa ggtcccctcc tgcaacacgc gcagcagccg
     1561 tacctggtcc tcgagcgtca gctcgccgac ctcgtcgagg aagagggtgc cgccgtcggc
     1621 cagctcgaat cggccgcgcg cctgctcggt ggcgccggtg aacgccccgc gctcgtggcc
     1681 gaagaggctg cttgcgacca ggcccggcgc gaacgaggcg atgtgcacgg cgatgaaagg
     1741 gcccgggcgt cgcggcgaca ggcggtggat ttcgcgcgcg atcagttcct tgccgacacc
     1801 ggtctcccct tcgatcagga cgctggtcac ggtcggcgcc actcggccga cgagttcgag
     1861 cgtttgccgc agcgccggcg actccccgac gatccggccg ccggccgacg gcagcgaggg
     1921 cgcctgccgg tagtagcggt tctcctgctc gaggcgccgc cgcgcctcta tcaggtcgcg
     1981 ccagatcgag aggttctcag cgaggactcc gagctgggct cccaggacgt cgagcagccg
     2041 ccggtccgac gctgacacca gcggccggct gtagcggttc tcgaacacgg cccagccgct
     2101 cttcggcagg cgcgtcgagc cgaacggcac gacgagcagc tgccccgtcc cacgcggtcc
     2161 atcgtcgagg aggggtgcgc cggtctccac ctcctccggc ggacgcgctg caagcagcga
     2221 ccgaacggcc tctacccagg cgggaccacc gccgcggctg gccacgacga gcggcgcctc
     2281 cgcgccccgc tcgacgatgg cacagcgctc gacgccgagc acggcgcaga gccgggcggc
     2341 gagatctccc cagccggtat ctccggcggc agcgttgaca ccgagccgcc ccagctcgag
     2401 gagcgccgtc gcaagctcga gcacgttacc cgaggcgggc gccaggtcga tcgaggcgaa
     2461 gacgcggcgc gactcgtcgt gcagccgttc cgcctccggc tgccggcccc ggcactcggc
     2521 ccactgcgcg gcgtcggcca gcgcgcgcgc cagttccgag gggcacgccc tcgactcgcg
     2581 cagcagcgcg acggcctgct cgtggtgggc cgagatccgg gccgcgactt cctcgtccaa
     2641 cgggcctcgt gcgaggcggc gagcccgaat ccggtgcgcg acggcccgca tgtagccgtc
     2701 gggccagccg agcatgcgca cgagttcggt ctcgacgtcg agcccggccg ggggcttcac
     2761 cccctcgacg tccatgcggt ccagcacctc gaacagggcg cttccgccga agaacatcag
     2821 ccccgaggcg accctcgcgc gctgcccggt ttcgagtacg tcggcggcgg ccgccagctg
     2881 cccttcgatc atccgtaccc aggcgagcga gatcgacgcg tgccagagga agtagggacc
     2941 cgtggggtcg ccggtccagt agcgcaccgc cgactcggcg tagagccgtg cgggctcgaa
     3001 ctcacgtcga ccggcgtgca ggcctgacag gaagcagtcg gccaggccga ccaggtgagg
     3061 gcggcccacg cgctcgccgg ccgaccggag ccggtggagc aggtcgagcg ctcggccgta
     3121 ctggcccacc tgcccgtagg tgctggcaag gatggcgagg ccggtttcgg ggaccgggtc
     3181 gagcagcggc tgcagttcgg gcggcagcgg aaggatctcc tggggcaggt cgccgagaag
     3241 ccgctcgaaa gtggccacgg cggccagcgc ctggccgacg aggatctgcc cgaaggcaac
     3301 ctgcaggtgg acctcgaagg agaccgcccg gctatcgagc gctcgcgccg cctcgagcgc
     3361 ccgttcgagg tactcgcctc cccggttcat gtcgcgtgcg gtgcggaccg actcgaagcc
     3421 gcgggcgccg caaaggatgg cctgccactc caggtttccg caggcgacgg acgcatcgat
     3481 ggctcggtcg aggatctccg gagtgagccg ggagccgaat cggaagcgtc gcacgcactc
     3541 gaccacgatc cccgcccagg cgtgggaacc gccgggaaac gactcggggg acgcagccag
     3601 gaccatgtga tggaacagtg ccgaagccac gtcctggcgg cgctgatcgg ctgcgcggcg
     3661 cccgttcgcc aggacggttg ggatcaggtc ggcgaggccg aacagccagg cccaatcgcc
     3721 cgggcctgcc gcgtcgagga ccctcgcctg gcgctcgacg tcgagcagca caaagtgacc
     3781 gggccccgcg gccgcgtcct cgccaatcca gccctggtcc cgccccccgt gaagccagcc
     3841 ccacaactcg gtcggccgaa cgatgtccca actgacgagg gcgtccagcg acaccgggcc
     3901 ggcggcggcc agcacgaatt ccgcagcccg tgtccccttc agccgccgtc cgtcggagcc
     3961 catcctccct cctcgcagtc ggcagtgttg tgcacccgca gcccgctgtc aactctcaac
     4021 gggttgtgag cacgtgcccc tcgagccgtt ggtctgcggg aacgcgcaag ctcgtttcca
     4081 gtgtggcgcg accacggcac gtcattctga cgcaagcact tgcatccagg ggaaccggtc
     4141 cgtcgcgtgg cggccccggg ccgggcgaca cgcggcacgt ctcctgccta tggtcgaggc
     4201 atgacgtacc gcattgaaac caaccaggcg gccagcgaag taagcctgtc cttccaaggc
     4261 ctgctggact ccgcggcgct cgacgcggtg aggtcgagcg tgacggccgc acgggcccag
     4321 ggggcgaagg cagtcgtgct cacgctcgcg ccggggacca cggtggaccg cgagtgcctt
     4381 ccggggctcc ggtcaatcga ggggctcgcc gtgaaggcgg cgtcccggtt tttggcaacc
     4441 tggctggccc agtgcggcgt gaactgactc gcactcgtgc tttttcggaa agggagcagc
     4501 aatcatgaag agcaagatga tcgtcgtcgt caccgcgttc ctcggtctcg cgatcgtgtc
     4561 gtccgcacag gcgcaggagg tacgcctgaa cgtcggggtc cgggggagct tcggggtgcc
     4621 gttcggggag gcacggggcg ggaccgacct gaaggacgtc accacccgcg ccatcccggt
     4681 gcaggtggac gtggactacc gcatcgacga gcactggagc gccggcgtct acgcgatcta
     4741 cgggccggtt gcggtggcgg acgcgacccg gcggcagctg gcggccagcg gccttcgcag
     4801 catcggcggt caccggcagc agcgcgtcgg cgtgcagctg acgcgcacgt tccgccccgc
     4861 ggcgcgggtg tcgccgtggg tcggcatggc cgccggctac gactggacgc gttacgcgtg
     4921 ggcgaagctg gcgacgggtg aggagaccga gatcggcgtg tcggggttcg agacgacgct
     4981 tcaggggggc gccagcttca agctgacgcc gcgcctcgcg atcggtccgt tcgccgcgtt
     5041 cgacctgggg cgctacgggc aggacatcgt ctgggtgcag cgcgccggca actcctcgac
     5101 gggcattccc gacaaggggc tgcaccagtg ggttcggttc ggcgtgaaga tgtcggtcgg
     5161 cttctagccg ccacgcgggc gggtgcgggg ggcggaggca cgggcctccg cccttccata
     5221 tgaaggcagt agaccgtccg gtcggaattg gcaccggagg gcctgtccag tcatcttggg
     5281 tacaatcgcc agagtgcctg ccggcagccc gggccgtttg ccttcggtcg atccgccagc
     5341 cagacaggag tgtcagatgc cactgaaccg acgacagttc ctcacgaccg cctcggcggc
     5401 acttgccgcc accacgctcg acccgcgcag cctgctggcc caggccgccg gacaggcgcc
     5461 gcccgagacg gccttcaagc tgcttcgccg cggtgtcggc atcttcaccg ggcagggagg
     5521 cacgatcggg tggttcgtgg accgcgaagg ggcgctcgtg atcgactcgc agttccccgc
     5581 caccgcgaag atctgcctgg agggcctgca gcagcggatg caggccagtg gcacaggcgt
     5641 ctcgcctgtg aagcaggcgc agggcggcac aggcgtctcg cctgtgaagc aggcgcaggg
     5701 cggcacaggc gtctcgcccg cgagcggcag gctgatcgac ttcctggtga acacccacca
     5761 ccacggcgat cacgtcgctg ggaacggcgt gttccagccg gccgtccgca aaatcctggc
     5821 gcacgtcaac gtgccgaaac tccagcggga ggcgtccgag cggcgcgggc ctggggcgcc
     5881 aggcccgtcg accgcgccgg tggttgcgaa cgcgaccttc gacaaggcat ggcacgagga
     5941 gatcgggcgc gaggagatgg aggcgcgtta ctacggcccg gcacacacca gcggcgacgc
     6001 cgtggtgatc ctcgagaagg cgaacgtggt gcacatgggc gacctcgtgt tcaaccggat
     6061 gcatccgttc atcgaccgcc ccgcgggagc ctcgatcgcc agctggatca cgctgctcga
     6121 ccgggtaccc gccgacatgc agcgcgacac gacctacatc ttcgggcacg gcaacccgaa
     6181 gttcggcgtg accggcacga gcgccgatct gaagtacatg cgcgactacc tggccgcact
     6241 gctcgacttc gtgcaggccg agatgaagcg gggccagccg cgcgatgcga tcgtcaggat
     6301 caccgacccg ctgaaaggtt tcgccgatca cggcccgctg gtcgagcgca cgctcgggcc
     6361 ggcgtacgac gagctggcgg ggtcgtagac acgagcggcg cagtacaatc cccccttctg
     6421 cccccctcca ggagcccgca tgccttcacg aatccgcctg gcgatcgggc ttgcgatcgc
     6481 catcgtcact ctcccgaacc tcgcacggct ggacggccag gaacagtacg accgaaccac
     6541 cctcatcccc gcgaagacgc tggccgcgat cgccaacgag gtgtctggcg cgcaggcgct
     6601 caaccacgtg atcgacatcg ccggctacga gcgcgatcgc agggcgtcgg agtacggcga
     6661 gggcctgccg cgcgaggccg aggtcgtcgc ctccattgcc agggaagccg ggctctcggc
     6721 ggtcgagatc ctgcggccgg ccggcgagcc gcggctgcag tgggacggtg aggaagggga
     6781 actgtggatc gaggagccga tcgcttcgcg ccgccttctc acccgctacc gcgacgtggc
     6841 cgcgacgctc gcccccaatt cgctcaccac cgaggtgacc gccgacctcg tgttcgtcgg
     6901 ccgcggcgac cgcgaggccg actacgaggg caaggacgtc aaggggaaga tcgtgctcgg
     6961 ctggggcagc ctgggggcga ttcacggctt ggcggtcacg cgctacggcg cggccggcgc
     7021 catcagctac ggcgtgagca gcggaaagcc gatcgaccgg cccgatcaga tcgcctgggg
     7081 cagcatcggg accttcccgc cggacggcga gaagaccggg ctgtcgcggg cgtcgtggtt
     7141 cggcttcaac ctgtcgcacc ggatgggcct gcagctcgtc gacctcctcg acaaggggac
     7201 ggtgcggctg cgtgcgaagg tgcgggcctc gcagtacccc gtgcagctgc cgactgtcac
     7261 ggcgaccatc ccgggcgacg gttccctggc cgccaccggc aaggacgagg ttgtcttcgt
     7321 cgcacacctg ttcgaggggg ccacaaagca gggggcaaac gacaacacca gcggcgtggc
     7381 ggtgcagctc gagatcgcgc gcgcctggat cgctctcgtg agaagcggcg ccctgcccgc
     7441 ccccaggcgg acgatccgct tcctctgggt gcccgagatc tcggggacga tggcgtacct
     7501 gcgagagcac cgggaggagg cgctccggat gctcgcggcc atcaacatgg acatggtcgg
     7561 ggcgaaccag acgctgcaca ggaacagcgc gaacctggcc taca
//
LOCUS       DAOPQX010000176         7581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10788_length_7581_cov_4.335504, whole genome shotgun sequence.
ACCESSION   DAOPQX010000176 DAOPQX010000000
VERSION     DAOPQX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_7581_cov_4.335504"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKK95_10275"
     CDS             <1..272
                     /locus_tag="PKK95_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18647.1"
                     /translation="QPLAEVLERLTGKAGHGIEIVSHVNDIPKGSRLAVSTSLLACLI
                     AVCMRATGQIHAIEGGLQENERRLVAARGGPLAAAHQAAVEGDEP"
     gene            269..952
                     /gene="nadD"
                     /locus_tag="PKK95_10280"
     CDS             269..952
                     /gene="nadD"
                     /locus_tag="PKK95_10280"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOC18648.1"
                     /translation="MSGSVRTGVMGGTFDPVHLGHLAAASAAADALSLPRVLFVPSHH
                     PPHRHEAPRVSAYHRFAMVALAVAEDARFEACDLELGRPGPSFTADTLRRLNALGYDP
                     SQLFFILGSDAVAEIATWHEYPAVLDLACFVVIARPGHAIGLIGSQVPDLAPRLVAPG
                     GAAAGPLKAGQIVLVPAATPDVSSTLVRDHLARGLDIEGLVPRGVEAYIGRHGLYRPE
                     PTPPGRQLA"
     gene            949..1323
                     /gene="rsfS"
                     /locus_tag="PKK95_10285"
     CDS             949..1323
                     /gene="rsfS"
                     /locus_tag="PKK95_10285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOC18649.1"
                     /translation="MTTRENRSATTADGLPSPVERAIEAARDKKAIDVALLDLRPLAS
                     FTDFFVICSGSNPRQVQAIAEAVEERLRDEGLRPSHVEGFDRAEWILLDYFDFIVHVF
                     SSARREFYALERLWGSAARVEV"
     gene            1565..2113
                     /locus_tag="PKK95_10290"
     CDS             1565..2113
                     /locus_tag="PKK95_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOC18650.1"
                     /translation="MLCKRCRRTPAGPVAVARAAGEYAGALRQLLHALKYEGRESLAP
                     PLAALMRERAPEVFEGASVVVPVPLHWQRRWQRGFNQAEALAAGLGVPVCRALVRRRS
                     TRAQFGLPAARRHRNVRGAFRPAWRGARRARSLLKGAIVVLVDDVATTGATLGACAEV
                     LKGMGAREVRAITAARAHARQS"
     gene            complement(2079..2663)
                     /gene="tmk"
                     /locus_tag="PKK95_10295"
     CDS             complement(2079..2663)
                     /gene="tmk"
                     /locus_tag="PKK95_10295"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOC18651.1"
                     /translation="MLIAFEGIDQSGKETQARLLGGRLSSAGRTVLTVSFPDYATPIG
                     TELRRALHGERDYGPDVIQLLMIANRYEWKPAMTAALDRGDVVIADRYLASSVAYGEA
                     QGLDPDWLFEAQRHLPQPDLTVMIDIAPATAVARKQAGRDRFEQDLPMLERVRASYRR
                     QAAAPGWAEVGGERAVTEVAAEVATIVERALARR"
     gene            complement(2701..3960)
                     /gene="lysA"
                     /locus_tag="PKK95_10300"
     CDS             complement(2701..3960)
                     /gene="lysA"
                     /locus_tag="PKK95_10300"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOC18652.1"
                     /translation="MTIATAGFIRDDTGTLSCSGVPLETIIARVGTPAYVYSGALVRQ
                     AWRRLDRVFAPVPHALHYALKANSTLALLRLLHAEGARADANSGGEIEVALRAGFTPD
                     QIVFTGVGKSDSELRRAVSLGLHAINAESPGEIGRIDAIARDAGTRARVAVRVNPDID
                     AGTHSHISTGRRHDKFGVPIELAPALYRDIARKPGLVPVGVHVHVGSQITDLAPLRRA
                     AESVVALAQALRADGIAVEHVDLGGGLGIPYDDSPVVTIEQYAHALVPIARSSGLALL
                     LEPGRVIVGAAGVLVATVVDIKGSGGGKTFAVLDAGMTDLLRPALYGAYHRIEAVRPH
                     AGGRRRYEVVGPLCESSDVVGRDRELPPLEVGDLVAILDAGAYGSAMSFTYNRRPLAA
                     EVLVDDDGWRLIRRRSTVDDSLALEME"
     gene            4044..4715
                     /locus_tag="PKK95_10305"
     CDS             4044..4715
                     /locus_tag="PKK95_10305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC18653.1"
                     /translation="MTANLLAAEPPSIDTLIERCLLGDQAAWETVVRQHWRKVFNVAY
                     KFVGSHEQAEDLTQDIFLKIFRSLETFDRRANFQTWLISVSRNLCIDHYRSVRRERQL
                     IDRELRSEDAIAVSRTASPLKTLENRDLAAELRQALQGLPAALRTAVLLRDIHELSYQ
                     EIAERLHLPEGTVKSRINRGRRELARRIQRLRAESSRPARPKGNGRTGAEPGHESELF
                     HEPDD"
     gene            4699..5040
                     /locus_tag="PKK95_10310"
     CDS             4699..5040
                     /locus_tag="PKK95_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOC18654.1"
                     /translation="MNLTTSRTGDVAVVRVGETRLMYPILSDFSQAVASLFAAGERKV
                     VIDLADVTYIDSASIGCLMDLYRQATHAGGRLKLSGVQKRVVTMLTMTGAHNFLEIHP
                     DEAAAVQSFGV"
     gene            5043..5327
                     /locus_tag="PKK95_10315"
     CDS             5043..5327
                     /locus_tag="PKK95_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18655.1"
                     /translation="MRTITTTTGAEIPLDGDLLAIIETLYEEVTVKRELNRSYEDMMG
                     EIRHLIAQMTTEERADYLAESLFHNTVRFENERLAAYLQKIAGGKKKVRR"
     gene            complement(5352..6266)
                     /gene="lpxC"
                     /locus_tag="PKK95_10320"
     CDS             complement(5352..6266)
                     /gene="lpxC"
                     /locus_tag="PKK95_10320"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOC18656.1"
                     /translation="MDSQRTLRRQVSCAGIGLHSGNKVTLSLKPAPANSGIRFRRSDL
                     GGLEIPATVDYLAGIHYATGLASKGGGVETVEHLLAALVGLGVDNAVVELNHPEVPIM
                     DGSAAPFVYLVQEAGMRVLPAPRQVLRVTRPVELARGDKRIAVYPCDHFRVSYSISFD
                     HPLLRHQARTVSLLGDAFTEQIAPARTFGFLKEVEMLRQNGLALGGSLENAIVIGETG
                     VLNNPLRFEDEFVRHKILDAMGDLALVGHPILGHVVAHRAGHALHTAFAAKLLESRDA
                     WELVELAAPLPAVAVPATASVKSAIAGR"
     gene            complement(6445..>7581)
                     /locus_tag="PKK95_10325"
     CDS             complement(6445..>7581)
                     /locus_tag="PKK95_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC18657.1"
                     /translation="EAGSPPRDSVAGSLTGAYLRGERSIETPAARRPADRGALVIRGA
                     RANNLHGIDVAFPLGVFTAVTGVSGSGKSTLVHEILYRALARQLYRAADEPGPHDAIE
                     GIELVDKVIEIDQSPIGRTPRSNPATYTGLFTFIRELFAVLPEAKARGFKPGRFSFNV
                     KGGRCETCQGDGVIAVEMHFLPNVYVTCEECKGRRYNRETLEIKYRGKSIADVLDLTV
                     AQALELLENFPAIANKLRTLAEVGLGYITLGQSATTLSGGEAQRVKLARELSRRGTGK
                     TLYILDEPTTGLHFEDTRKLLDVLGRLVDQGNTVVVIEHNLDVIKCADHIVDLGPEGG
                     ADGGRVVAVGTPEAVAGVRQSHTGRFLRELFDRRPAARARRWAG"
BASE COUNT         1111 a         2725 c         2620 g         1125 t
ORIGIN      
        1 ggcagccgct ggcggaagtg ctggaacgcc tcaccggaaa agccgggcac ggcatcgaga
       61 tcgtcagcca cgtgaatgac attcccaagg gatcgcggct ggccgtttcg accagcctgc
      121 tggcatgcct gatcgcggtg tgcatgcggg cgacgggcca gatccacgcg atagaaggcg
      181 gtttgcagga gaatgagcgg cggctggtgg cggcgcgcgg cggaccgctg gcagccgcgc
      241 accaggcggc cgtcgagggt gacgagccat gagcggcagc gtgcgcacgg gagtcatggg
      301 cgggaccttc gaccccgtcc acctggggca cctcgccgcg gcctctgctg ccgccgacgc
      361 gctgagcctc ccccgcgtgc tcttcgttcc gtcgcaccac ccccctcacc ggcacgaggc
      421 gccccgcgtc tcggcgtatc atcggttcgc gatggtcgcg ctcgccgtgg ccgaagacgc
      481 gcggttcgag gcctgcgacc tcgagctcgg acggcccggc ccgtccttca cggccgacac
      541 gctgaggcgg ctgaacgccc tcgggtacga cccctcgcag ttgttcttca ttctcggcag
      601 cgatgccgtt gctgaaattg cgacctggca cgaatacccg gcggtgctcg acctggcgtg
      661 cttcgtcgtc atcgcgcgtc cgggccacgc gatcggtctg atcggctcgc aggtgccgga
      721 cctggcgccc cgcctggtgg cgccgggcgg cgccgcggcg ggcccgctga aagccggcca
      781 aatcgtgctg gttccagcgg cgacccctga cgtctcgtcc accttggtgc gcgaccacct
      841 ggcgcgcggc ctcgatatcg aggggctcgt cccccgcggg gtcgaggcgt acatcggccg
      901 gcacgggctg tatcgacccg aacccacgcc ccctggacga cagcttgcat gacgacacgc
      961 gagaacagga gcgccaccac ggcggacggg ctgccttcac cggttgaacg ggcgatcgag
     1021 gccgcgcgcg acaagaaggc catcgacgtc gcgctgctcg atctgcgccc gctggcgagc
     1081 tttaccgact tcttcgtgat ctgctcgggc tcgaacccca ggcaggtgca agcgatcgcg
     1141 gaggcggtgg aagagcggct gcgggacgag gggcttcgcc cgtcgcacgt cgaggggttc
     1201 gatcgcgccg agtggatcct gctcgactac ttcgacttca tcgtccacgt cttcagcagc
     1261 gcgcggcgcg agttctatgc gctcgaacgc ctctggggca gcgcggctcg cgtggaggtc
     1321 tagccgccgg gctgccgacg ggctcgtcgc agccgtgctg gccccccggt gcctcgcgtg
     1381 cggtctcccg ctcgacgcgc ccacgcgcgg ggccgtctgc cagccctgct ggcaatcgat
     1441 cctccctctc gccccgccgt tctgctgccg ctgcggcgac tcgctggcaa accgctggcg
     1501 gccggcttca gttcctgcaa cacccgatcg cgagtcgcag gcgacgctcg tcgcggccga
     1561 cagcttgctg tgcaagcgat gccggcgcac gcctgccggc ccggtggccg tggcgcgggc
     1621 ggcgggggag tacgcgggcg cgctgcggca gctgcttcac gcgctgaagt acgagggccg
     1681 ggaatcgctc gcgccccctc tggcggccct gatgcgcgaa cgggcgcccg aggtgttcga
     1741 gggggcctcg gtcgtcgtgc cagtgccgct gcactggcag cggcgctggc agcgcggctt
     1801 caaccaggcc gaggccctgg cggccggcct cggcgtcccc gtgtgccgcg cgctcgttcg
     1861 gcggcggtcg acgcgggcgc agttcggcct tcccgccgcc cggcgtcacc gcaacgtccg
     1921 gggcgcgttc cgcccggcct ggcgcggagc gcggcgcgcc cgttccctgc tgaagggggc
     1981 aatcgtggtc ctggtcgacg acgtggcgac gacgggggcc acgctgggag cctgcgcgga
     2041 ggtcttgaag gggatggggg cgagagaggt tcgagcaatc accgccgcgc gagcgcacgc
     2101 tcgacaatcg tagcgacctc ggccgccacc tccgtcacgg cgcgctcgcc gccgacctcc
     2161 gcccagccgg gcgcggccgc ctggcggcgg tagctcgcgc gcacccgctc gagcatcggc
     2221 aggtcctgct cgaaacggtc gcggcccgcc tgcttgcggg cgactgccgt cgccggcgcg
     2281 atgtcgatca tcacggtgag atcgggctgc ggcaggtgcc gctgggcctc gaacagccag
     2341 tcagggtcga gcccctgcgc ctcgccgtac gccacgctcg acgcgaggta gcgatcggca
     2401 atcaccacgt cgccgcgatc gagggcagcc gtcatggccg gcttccactc gtagcggttg
     2461 gcgatcatca gcagctggat gacgtcgggg ccgtagtcgc gctcgccgtg cagcgcacgc
     2521 cgcagctcgg tgccgatcgg ggtggcgtaa tcgggaaacg agacggtcag caccgttcgc
     2581 ccggccgacg acaaccgccc gccgagcagc cgcgcctgcg tctccttgcc gctctggtcg
     2641 atcccctcga acgcgatcag catctccccc gccttccgcc ttcgctcctc cgccttccgc
     2701 tcactccatc tccagcgcca ggctgtcgtc caccgtcgag cgccggcgga tcaaccgcca
     2761 gccgtcgtca tcgaccagca cctcggcggc gagcggccgg cggttgtagg tgaacgacat
     2821 ggccgacccg taggcgccgg catcgaggat ggcaaccagg tcgcccacct cgaggggcgg
     2881 cagctcacgg tcgcggccca ccacgtcgct cgactcgcac aaggggccga cgacctcgta
     2941 gcgccggcgg ccgccggcgt gcggccgcac ggcctcgatc cggtgatacg cgccgtagag
     3001 ggcgggtcgc agcaggtcgg tcatcccggc gtcgagcacc gcgaacgtct tgccgccgcc
     3061 agaccccttg atgtccacga cggtggccac cagcaccccc gccgctccga cgatcacccg
     3121 gccgggctcg agaagcaggg ccaggccgct cgatcgggcg atcggcacga gggcgtgggc
     3181 atactgctcg atcgtcacga cgggcgagtc gtcgtacggg atgccgaggc cgccgccgag
     3241 atccacgtgt tcgacggcga tcccgtcggc acggagcgcc tgcgccaggg cgaccaccga
     3301 ctcggcggcg cggcgcagcg gggcgaggtc ggtgatctgc gaccccacgt ggacgtgcac
     3361 gcccaccgga acgagccccg gcttccgggc gatgtcgcgg tacagggccg gcgccagctc
     3421 gatgggcacg ccgaacttgt cgtggcggcg gccggtggag atgtggctgt gcgtcccggc
     3481 gtcgatgtcc gggttcacgc gcacggcaac gcgcgccctc gtcccggcgt cgcgggcgat
     3541 cgcgtcgatg cggccgatct cgccgggcga ctcggcgttg atcgcgtgaa ggccgagcga
     3601 caccgcccgc cgcagctcgg aatcggactt gccgacaccg gtgaagacga tctgatcggg
     3661 ggtgaacccg gcccgcaggg cgacctcgat ctcgccgccc gagttggcgt cggcgcgcgc
     3721 cccctcggcg tgcagcaggc gcagcagcgc gagcgtcgag ttcgccttca gcgcgtagtg
     3781 cagcgcgtgg ggaaccgggg cgaaaacccg gtcgaggcgc cgccatgcct ggcggaccag
     3841 cgcgccgctg tagacgtagg caggggtgcc cacccgcgcg ataatcgttt cgaggggaac
     3901 gccggagcag gagagggttc cggtgtcgtc gcggatgaac ccggcggtcg cgatggtcac
     3961 gtccgcgcat tgtatccgag cggatctcgg tttgacatgc cgaaaaacgc tttgctatca
     4021 tccgctttcc cgcccctcgc cacatgacgg ccaacctcct cgcagccgaa ccgccgtcga
     4081 tcgacacgct gatcgagcgc tgcctgctcg gcgaccaggc cgcatgggag actgtcgtcc
     4141 gccagcactg gcggaaggtg ttcaacgtcg cctacaagtt cgtcggcagc cacgagcagg
     4201 ccgaggacct gacccaggac atcttcctca agatcttccg ctcgctcgag accttcgacc
     4261 ggcgcgcgaa cttccagact tggctcatca gcgtcagccg caacctctgc atcgatcact
     4321 accgcagcgt ccggcgcgag cggcagctca tcgaccgcga gctgcgctcc gaggatgcga
     4381 ttgccgtctc gcggaccgcg agcccgctca agacgctcga gaaccgcgat ctcgccgcgg
     4441 agctgcggca ggcgctgcaa gggttgcccg ccgcactgcg gaccgccgtg ctgctgcgcg
     4501 acatccacga gctgtcgtac caggagattg ccgaacggct gcacctcccg gaggggaccg
     4561 tgaagtcgcg gatcaaccgc ggccgccggg agctggcgcg gcgcatccag cgcctgcgcg
     4621 ccgagtcgag ccggccggcc cggcccaagg gcaacgggcg caccggcgcc gagccggggc
     4681 acgaatcgga gttgttccat gaacctgacg actagtcgca cgggagacgt ggcggtggta
     4741 cgcgtcggtg aaacccgctt gatgtacccg atcctctccg atttctccca ggcggtcgcg
     4801 tcgctgttcg ccgccggaga acggaaggtc gtcatcgacc tcgccgatgt cacctacatc
     4861 gacagcgcga gcatcgggtg cctgatggac ctgtaccgcc aggccacgca cgcgggaggg
     4921 cggctcaagc tctcgggcgt ccagaagcgc gtggtgacga tgctcaccat gaccggcgcc
     4981 cacaacttcc tcgagatcca tcccgacgag gcggctgccg tccagagctt cggggtgtag
     5041 acatgcggac catcaccacg accacgggcg ccgagatccc gctcgacggc gacctgcttg
     5101 ccatcatcga gacgctgtac gaggaagtca ccgtcaagcg cgagctgaac cggtcctacg
     5161 aggacatgat gggcgagatc cgccacctga tcgcccagat gacgaccgag gagcgcgccg
     5221 actaccttgc ggagagcctc ttccacaaca cggtgcgctt cgagaacgag cgcctcgcgg
     5281 cctacctgca gaagattgcc ggcgggaaga agaaggtgcg gcggtgaggg gcggcgggaa
     5341 cccgccgcct gctacctccc cgcgattgcg ctcttgaccg atgcggtcgc cggaaccgcc
     5401 accgccggca gcggcgccgc cagctccacc agctcccacg cgtcgcgcga ctcgagcagc
     5461 ttcgccgcga aggccgtgtg cagggcgtgg cccgcacggt gcgccaccac gtgccccagg
     5521 atcgggtgcc ccaccagcgc caggtcgccc atcgcgtcca ggatcttgtg gcgcacgaac
     5581 tcgtcctcga accgcagcgg gttgttgaga acgccggtct cgccgatgac gatcgcgttc
     5641 tcgagcgacc cgcccagcgc gaggccgttc tggcgcagca tctcgacctc cttcaggaag
     5701 ccgaacgtcc gcgccggcgc gatctgctcg gtgaacgcgt cgcccagcag gctcacggtg
     5761 cgtgcctggt ggcgcagcag cgggtgatcg aagctgatcg agtagctcac ccggaagtgg
     5821 tcgcagggat agacggcaat ccgcttgtca ccccgcgcca gctcgaccgg gcgggtcacc
     5881 cgcagcacct gccgaggcgc gggcagcacc cgcatccctg cctcctgcac gaggtacacg
     5941 aacggcgccg cgctgccgtc catgatcggc acttcggggt ggttcagctc gacgaccgcg
     6001 ttgtcgaccc ccaggcctac gagcgccgcc agcaggtgct ccaccgtctc gaccccgccc
     6061 cccttggagg ccagcccggt cgcgtagtgg atgccggcaa ggtagtcgac cgtggccggg
     6121 atctcgagcc ccccgaggtc ggagcgccgg aaccggatgc cggaattggc cggagcgggt
     6181 ttgagggaaa gagtgacctt gttgccggag tggaggccga tcccggcgca ggaaacctgc
     6241 cgtcgaagcg tccgttgtga gtccattagc gtctcatgcg cgccaacacg ccgccgccgt
     6301 ggccggccat acctcagcaa tcaccatacc ggtgagccat ccgtgtggag tttctatgga
     6361 aatgctgaca gataaggact tgccgcctca cacccgactg ccacaccgtc ccgctgacga
     6421 actgtggctg tttgaccaca tgactcagcc cgcccaccgc cgcgccctcg cggccggccg
     6481 gcggtcgaac agctcccgga ggaaccgtcc agtgtgcgat tgtcgcacac ccgcgacggc
     6541 ctcgggggtt cccacggcta ccacccgccc tccgtcggcc cccccctcgg gcccgaggtc
     6601 cacgatgtgg tcggcgcact ttatcacgtc caggttgtgc tcgatcacga ctacggtgtt
     6661 cccctggtcc accagccggc cgagcacgtc gagcagcttc cgggtgtctt cgaagtgcaa
     6721 ccccgtggtc ggctcgtcga ggatgtagag cgtcttcccg gtgccccggc gcgacagctc
     6781 ccgtgccagc ttgacgcgct gtgcctcgcc gccgctgagc gtcgtcgctg actggcccag
     6841 ggtgatgtag ccgagcccca cctcggcgag cgtgcgcagc ttgttggcga tggcggggaa
     6901 gttctcgagc agctcgagcg cctgggcgac ggtcaggtcg agcacgtccg cgatcgactt
     6961 gccgcggtac ttgatctcga gcgtctcccg gttgtagcgc cgccccttgc actcctcgca
     7021 cgtcacgtag acgttgggca ggaagtgcat ctccaccgcg atgacgccgt cgccctggca
     7081 ggtctcgcag cgccccccct tgacgttgaa cgagaagcgg ccgggcttga agccgcgcgc
     7141 cttcgcctcg ggcagcaccg cgaacagctc gcggatgaac gtgaagagtc ccgtgtaggt
     7201 ggcggggttc gagcggggcg tgcgtccgat gggcgactgg tcgatctcga tcaccttgtc
     7261 caccagctcg atcccctcga tcgcgtcgtg cggccccggc tcgtcggcgg cgcggtacag
     7321 ctgtcgcgcg agcgcgcggt acaggatctc gtggaccagg gtcgacttcc ccgacccgct
     7381 cacgccggtg acggccgtga acaccccgag cgggaacgcg acgtcgatgc cgtgcaggtt
     7441 gttggcgcgg gcgccgcgga tgacgagggc gccgcgatcg gccggccggc gggcggcggg
     7501 cgtctcgatg ctgcgctcgc cgcgcaggta ggcccccgtc agcgagccgg ccacgctgtc
     7561 gcgcgggggg ctcccggcct c
//
LOCUS       DAOPQX010000177         7552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10842_length_7552_cov_4.324263, whole genome shotgun sequence.
ACCESSION   DAOPQX010000177 DAOPQX010000000
VERSION     DAOPQX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10842_length_7552_cov_4.324263"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PKK95_10330"
     CDS             <1..186
                     /locus_tag="PKK95_10330"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18658.1"
                     /translation="VDELAARFGRPSELSLERHMGCGMGGCYSCVVRVRTPDGGSRYA
                     RTCIEGPVLRGDEVLWE"
     gene            177..1100
                     /locus_tag="PKK95_10335"
     CDS             177..1100
                     /locus_tag="PKK95_10335"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOC18659.1"
                     /translation="MGVDLSVQLGPLRLRTPLMSASGCFGYGVEYAGAVDLASLGAVV
                     VKGLFLNERDGHPPPRIVETPAGMLNAIGLQGIGVHRFVREKMPELRRIGATVIVNVC
                     GTTVEEYVEVSKVLSDTEGVAALELNISCPNIKEGGISFGCNLAGTHRVVSAVRRATS
                     LPIIPKLTPNVTDVASFAAAAEEAGADAVSLVNTFLAMAIDVETRRPKLSNGMGGLSG
                     PAIRPIAVRMVYECARIVKIPVIGIGGIASAADALEFLIAGATAVQVGTANFVDPFIW
                     GKLEAGLRSYLERHGVSRVADLVGSLQVGSS"
     gene            1100..1819
                     /gene="pyrF"
                     /locus_tag="PKK95_10340"
     CDS             1100..1819
                     /gene="pyrF"
                     /locus_tag="PKK95_10340"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOC18660.1"
                     /translation="MQELIVALDVDTADAARALAAKLAGTVGGFKVGSRLFTSEGPDF
                     VRALVGEGHRVFLDLKYHDIPNTVAGAVGAAARLGVWMLNVHASGGRAMMRAAVKAAD
                     EAAAAAGGARPLVIAVTVLTSLDRPALEEVGVSIPVDDQVEHLAALAEQAGLDGVVAS
                     ARETAMLRANRRDGFLIVTPGIRGPASPGASADDQARTMGAVAALAAGASYLVVGRPI
                     IEASDPRGAAERIAEEVAGAR"
     gene            complement(1908..2477)
                     /gene="pal"
                     /locus_tag="PKK95_10345"
     CDS             complement(1908..2477)
                     /gene="pal"
                     /locus_tag="PKK95_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOC18661.1"
                     /translation="MNRFARYLVVAGTVAVVGLGATGCKKQAPETAPPPPPPAAAPAP
                     PPPPPPPPPPPPPPAPLTEEEIFAKKTLDELNAERPLADVFFDYDKSDLKDEARSSLQ
                     KNADWLKKWTSTKITVEGHCDSRGTAEYNLALGERRATAVKDYLVTLGVPADRVLVIT
                     KGKEQPFCTQETDECWAQNRRGHFIITAK"
     gene            complement(2668..3528)
                     /gene="larE"
                     /locus_tag="PKK95_10350"
     CDS             complement(2668..3528)
                     /gene="larE"
                     /locus_tag="PKK95_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOC18662.1"
                     /translation="MSTAPARSEARRAADSLADRERRLRAILVELGSVVVAFSGGVDS
                     AYLAVTAADTLGDRALAVTADSPSYPSSHREVALDLVSRFGLRHEILRTEEIERPEYR
                     ANPANRCYYCKDELFGRLTALARERGFAAVADGNNADDRGDYRPGRQAAREHGVRSPL
                     DEAGLSKADIRELSRKRGLPTWDQPASACLSSRIPYHSEVTVEKLRMIERAEDVLHSF
                     GFRQCRVRHHGELARIELSPDEMPRALESEVREGLIGELKAIGFRYVSLDLQGYRQGS
                     LNEALILRPA"
     gene            complement(3528..3752)
                     /locus_tag="PKK95_10355"
     CDS             complement(3528..3752)
                     /locus_tag="PKK95_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18663.1"
                     /translation="MATCPECDADVEVDEYDVNKGDLVTCPECGSDLEVTSVSPIELE
                     AVSDEDEDELDEEDEDELDDEDEDEEEDWE"
     gene            3919..4632
                     /locus_tag="PKK95_10360"
     CDS             3919..4632
                     /locus_tag="PKK95_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HOC18664.1"
                     /translation="MTAGTAAKTVLIADDTAFVRERFQTALHEAGHRALAVKSAAELL
                     ARVRADLEAIDLLVVDLRLPHAAGVELIRSVRKLDQGKLPILVFSGTISSAEEVRELA
                     ALGVAGYVNEYSAVQHILPSLAPHLFPDNFNRRSSPRVVLGIPVAYRSGDTITAVLTL
                     NLSKGGIGIRTMNPLAEGIRVRLRFRLPGARREIEAEARVAWSDRRIGMGLQFEKVQA
                     EDQAAIDEFVDTHFFNTRQ"
     gene            complement(4745..6889)
                     /locus_tag="PKK95_10365"
     CDS             complement(4745..6889)
                     /locus_tag="PKK95_10365"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOC18665.1"
                     /translation="MSRANVAECPVCEVHTESVYRVDGMDCHEEVALLERHLRHLPGL
                     ERFSADVVSGRLRVAHDAARLSASQVAEAVAATGMRAWLEHEQPVAASIGTGRRRVFV
                     VAASGAAFLAGLAAELAGGPRPLAYLLYAVSIALAASLTARRALAAARSASFDINVLM
                     LVAVAGALVLGEWSEAAAVIFLFALAQLLETRSMERARRAIRSLMELTPADALVRRPE
                     GERRLPLDEVEVGDTLVVRPGERIALDGVVAAGASEVNQAPITGESMPVDKAQGDGVF
                     AGSVNGHGALEVRVTRLSGDTTMARIIALVEVAQAQRAPSQAFVERFARYYTPAVLAT
                     AAAIAVLPSLAFGQPFGPWFYRALVLLVISCPCALVISTPVSFVSALAAAARRGVLVK
                     GGVHLERAAALGAMAFDKTGTLTRGRPEVVGVQPLNGTSPERLLGLAAALEARSEHPI
                     ARAIIERAAAGAVAPPPGEAFRALPGLGAEASIEGAPVLVGNHRLFEARGLCSPAIDA
                     RLEELGRQGTTAVLVARGNLPIGIIGVADKTREAGRSAILELRRAGLAHLVMLTGDHE
                     RTARAIADGLGVDEVRAELLPGDKVEAVKELRRRFGSVAMVGDGVNDAPALAAADLGI
                     AMGAAGSGAALETADVALMSDDLLKLPYLVRLGRRTLFNVKTNVAFSLGVKALFLALA
                     VTGAATLWMAVAADMGASLLVVANGLRLLRTE"
     gene            complement(6886..7314)
                     /locus_tag="PKK95_10370"
     CDS             complement(6886..7314)
                     /locus_tag="PKK95_10370"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC18666.1"
                     /translation="MVTTATGAASPVRRAVSNSDAADACEVFVADAGRVAAVRAALVG
                     TGSVELLAETFRVLGDPTRVRILDALSHAELCVCDVATLLGLSQSAVSHQLRVLRGLR
                     LVRSRREGRMVFYALDDDHITGLFREGLRHVEEASARERP"
     gene            7349..>7552
                     /locus_tag="PKK95_10375"
     CDS             7349..>7552
                     /locus_tag="PKK95_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC18667.1"
                     /translation="MLRFQLKSKALNDRSTPPLGPREHGVIEVQHLTKRYGRVSAVED
                     ISFRVERGEILGFLGPNGAGKTTT"
BASE COUNT         1099 a         2690 c         2665 g         1098 t
ORIGIN      
        1 gtcgacgaac tggccgcccg cttcgggcgc ccctcggagc tgtccctgga gcggcacatg
       61 ggatgcggca tgggcggctg ctacagctgc gtcgtccgcg tgaggacgcc ggacggcggc
      121 agccgctacg cgcgcacctg catcgagggg ccggttctca ggggagacga ggtgctgtgg
      181 gagtagacct ttcggtccag ctggggccgc tgcgcctgcg gacgccgctg atgtcggcga
      241 gcgggtgctt cggctacggc gtcgagtacg ccggcgccgt ggacctcgcc agcctcggcg
      301 cggttgtcgt caagggcctg ttcctcaacg agcgagacgg acaccctccg ccgcgcatcg
      361 tcgagacccc ggccggcatg ctgaacgcga tcggccttca ggggatcggc gtgcaccgct
      421 tcgtgcggga gaagatgccc gagctgcggc ggatcggggc gaccgtgatc gtgaacgtgt
      481 gcgggaccac ggtcgaagag tacgtggagg tctccaaggt cctctccgat accgagggag
      541 tcgccgcgct cgagctgaac atctcctgcc cgaacatcaa ggaggggggc atctcgttcg
      601 gctgcaacct ggcgggcacc caccgggtgg tgtcggcggt gcgccgcgcg acgagcctgc
      661 cgatcatccc caagctgacg cccaacgtca ccgacgtggc gtcgttcgcc gccgcggccg
      721 aagaggccgg ggccgacgcg gtctcgctcg tcaatacctt tctcgcgatg gcgatcgacg
      781 tcgagaccag gcggccgaag ctctcgaacg ggatgggggg gctgagcggt cccgccatcc
      841 ggccgatcgc cgtgcggatg gtgtacgagt gcgcgcggat cgtgaagatc ccggtgatcg
      901 ggatcggcgg cattgcgtcg gcggcggacg ccctcgagtt cctgatcgct ggcgcgaccg
      961 ccgtccaggt gggaaccgcg aacttcgtgg atccgttcat ctgggggaag ctcgaggcgg
     1021 ggctgcgctc gtacctggag cggcacggcg tttcccgcgt ggccgacctg gtcggctcgc
     1081 tgcaggtggg gagctcgtga tgcaggaact gatcgtcgcc ctcgacgtgg acacggcgga
     1141 cgcggcgcgg gcgctggccg cgaagctcgc ggggacggtg ggcggcttca aggtcggcag
     1201 ccgcctcttc acctcggagg ggcccgactt cgtccgcgcg ctcgtcggcg agggacaccg
     1261 ggtgttcctc gatctgaagt accacgacat cccgaacacc gtggccggtg cggtgggcgc
     1321 cgcggcccgg ctcggcgtct ggatgctcaa cgtccacgcc tccggggggc gcgcaatgat
     1381 gcgggccgcg gtgaaggcgg ccgacgaggc cgctgccgcc gcggggggcg cgcgcccgct
     1441 ggtgatcgcc gtgacggtgc tcaccagcct cgatcgcccc gcgctcgaag aggtcggcgt
     1501 ctcgatcccc gtggacgacc aggtggagca cctggcggcg ctcgccgaac aggcgggact
     1561 cgacggggtg gtcgcctcgg cgcgcgagac ggcgatgctg cgggccaacc gacgtgacgg
     1621 gttcctgatc gtgacgcccg gcatccgcgg gccggcgtcg cccggcgcaa gcgccgacga
     1681 ccaggcgcgg acgatgggtg ccgtggccgc actcgcagcc ggggcgagct acctggtggt
     1741 gggccggccg atcatcgagg cgtcagaccc gcgcggggcg gcggagcgca tcgccgaaga
     1801 ggtggccggc gcccgctgaa cgcctcccgc cctccgcctt cggtgtacac gaaaaggggc
     1861 gcaagccgga gagcttgcgc ccctttcgtc gtttgcgatg aagccgacta cttcgccgtg
     1921 atgatgaagt ggccgcggcg gttctgcgcc cagcactcgt cggtttcctg cgtgcagaag
     1981 ggctgttcct tccccttcgt aatcaccagg acgcggtcgg ccggcacgcc gagcgtgacg
     2041 aggtagtcct tcacggcggt tgcgcggcgc tcgccgaggg cgaggttgta ctccgcggtg
     2101 ccgcgcgagt cgcagtgccc ctcgacggtg atcttcgtcg aggtccactt cttcagccag
     2161 tcggcattct tctgcagcga ggaccgggcc tcgtccttca ggtccgactt gtcgtagtcg
     2221 aagaacacgt cggccagcgg cctctcggcg ttcagctcgt cgagggtctt cttggcgaag
     2281 atctcctcct cggtcagcgg tgcgggcggc ggcggcggtg gcggcggcgg tggcggtggc
     2341 ggtggcggcg cgggtgccgc ggctggcggc ggcggcggag gcgccgtctc gggcgcctgc
     2401 ttcttgcagc cggtggcgcc gaggccgacc acggcaacgg tcccggccac gaccaggtac
     2461 cgggcgaaac ggttcatctc cacatctcct ggacggccaa ccgcgcgcgg cgtgtgtttt
     2521 cagtatcggg gggtcaccga cgaagctcgg cgccggtggc gggttggacg gagttcagct
     2581 tatgttgaac ccagctctag tgtcaaaagg aaaccgcacg gcccgcgaac gcgcgtgcct
     2641 cgtggggccg ggagttgggc actccgctca cgcggggcgg aggatgagcg cctcgttcag
     2701 gctgccctgg cggtagccct ggagatcgag gctgacgtag cggaacccga tcgccttcag
     2761 ctcgccgatc agcccctccc gcacctcgga ctccagcgcc cgcggcatct cgtccgggga
     2821 cagctcgatg cgggccagct cgccgtgatg gcgcacccgg cactgccgga acccgaacga
     2881 gtggagcacg tcctcggcgc gctcgatcat ccgcagcttc tcgaccgtga cctcggagtg
     2941 ataggggatt cgcgacgaga ggcaggctga ggcaggctgg tcccaggtcg gcagccccct
     3001 tttccgcgac agctcgcgga tgtcggcctt cgagaggccg gcctcgtcga gcgggctgcg
     3061 gacgccgtgc tcgcgcgcgg cctgccggcc cggacggtag tcgccgcggt cgtcggcgtt
     3121 gttcccgtcg gccacggccg caaacccgcg ttcgcgggcg agcgcggtga ggcgcccgaa
     3181 cagctcgtcc ttgcagtagt agcaccggtt ggccgggttg gcgcggtact cgggccgctc
     3241 gatctcttcg gtccggagta tctcgtgccg caggccgaac cggctgacca ggtccagcgc
     3301 cacctcccga tggctcgagg ggtagctggg gctgtcggcg gtgaccgcga gcgcgcggtc
     3361 accgagcgtg tcggccgccg tcaccgcgag gtaggcgctg tccacgccgc cgctgaaggc
     3421 gacgaccacc gacccgagct caacgaggat cgcgcggaga cgccgttcgc ggtcggccag
     3481 cgagtcggcc gcgcggcgcg cttcacttct ggcgggggct gtagacacta ttcccaatcc
     3541 tcttcctcgt cttcgtcctc gtcgtccagc tcgtcctcgt cttcctcgtc cagctcgtcc
     3601 tcgtcctcgt cggaaacggc ctccagctcg atcggcgaga cgctggtcac ctcgaggtcg
     3661 gatccacact cggggcaggt gaccaggtca cccttgttca cgtcgtactc gtccacctcg
     3721 acgtccgcgt cgcactcggg gcacgtagcc attcccgatc ctcctggcgg cttgctccgc
     3781 cgatcgacgc ccggcccgcg gcaccacgcc ccgcccccgg gcaaaggcag ggattatagc
     3841 gacaccctcc ggactcccgc aaaccgatta ccgtcgcggg gctaacgtcc caccgggcag
     3901 gtcccgatac accaagcgat gaccgcgggg accgctgcca aaaccgttct catcgccgac
     3961 gacacggcct tcgtccggga gcggttccag accgcgctgc acgaagcggg tcaccgcgca
     4021 ctggctgtca agagcgcggc ggagctgctg gcccgagttc gcgccgacct ggaggccatc
     4081 gacctgctgg tcgtcgacct tcgtctgccc cacgccgccg gcgtcgagct gatccgctcg
     4141 gtccgcaagc tggaccaggg caagctgccg atcctcgtct tcagcgggac gatctcgagc
     4201 gccgaagagg tccgggaact ggccgccctg ggtgtggccg gctatgtcaa cgagtatagc
     4261 gccgtccagc acatcctgcc ctcactggcg ccccacctgt tccccgacaa cttcaaccgg
     4321 cggtcgagtc cccgggtggt cctgggaatc ccggtggcgt accggtcggg cgacaccatc
     4381 acggcggtcc tgacgctgaa cttgagcaag gggggcatcg gcatccgcac gatgaacccg
     4441 ctggcggagg ggatccgcgt ccgcctgcgg ttccggctgc ccggcgccag gcgcgagatc
     4501 gaggccgagg cccgggtggc gtggagcgac cggcgcatcg gcatgggcct gcagttcgag
     4561 aaggtgcagg ccgaagacca ggccgcgatc gacgagttcg tcgacaccca cttcttcaac
     4621 acccgccaat agcgccggtt tccccagtag cgccggtgtc cgtgccggcc tggccttccc
     4681 ccggtatccc cggcctccgt gccggcgggt gtagggtgta cattggcctc tcctccaggg
     4741 gaagtcactc agtgcgcagc agacgcaggc cgttggcgac gacgagcagc gaggcgccca
     4801 tgtcggcggc gacggccatc cagagggtcg cggcgccggt gacggcgagc gcgaggaaga
     4861 gggccttgac gccgagcgag aaggcgacgt tggtcttgac gttgaacagc gtgcggcggc
     4921 cgaggcgcac gagatagggc agcttcaaca ggtcgtccga catcagcgcg acgtcggcgg
     4981 tctcgagcgc ggcacccgat cccgccgccc ccatcgcgat tcccaggtcg gcggcggcga
     5041 gcgcgggggc gtcgttcacg ccgtcgccca ccatggcgac cgagccgaag cggcggcgca
     5101 gctccttcac cgcctcgacc ttgtcgcccg gcagcagctc ggcccgcacc tcgtcgacac
     5161 cgagcccatc ggcaatcgcc cgcgccgtgc gctcgtggtc gccggtcagc atcaccaggt
     5221 gcgcgaggcc ggcgcggcgc agctcgagga tcgccgagcg gcccgcctcc cgggtcttgt
     5281 cggccactcc gatgatcccg atcggcaagt tccctcttgc gacgagaacg gccgttgtcc
     5341 cctgccgccc gagctcctcg agccgcgcgt cgatggccgg cgaacacagc ccgcgcgcct
     5401 cgaacagccg gtggttcccc accagcaccg gcgccccttc gatgctcgcc tcggcgccca
     5461 gcccgggaag ggcgcggaag gcctcgccgg gcggcggggc gaccgccccg gcggctgctc
     5521 tctcgatgat tgcgcgggcg atcgggtgct ctgacctcgc ctcgagggcg gcagccagtc
     5581 ccagcagccg ctccggagac gtcccgttca gcggctgcac gccgaccacc tcgggccgtc
     5641 cgcgcgtcag cgtgccggtc ttgtcgaagg ccatggcccc gagcgcggcc gcgcgctcca
     5701 ggtgcacgcc ccccttgacg aggacaccgc ggcgcgccgc ggcggccagc gccgacacga
     5761 aggacaccgg cgtcgagatg acgagcgcgc acgggcagga gatgacgagc agcaccagcg
     5821 cccggtagaa ccagggcccg aacggctgcc cgaacgcgag ggacggcagg acggcaatcg
     5881 ccgcagccgt cgcgagcacc gcgggcgtgt agtaacgcgc gaaccgctcc acgaaagcct
     5941 gcgagggcgc gcgctgcgcc tgcgcgactt ccacgagcgc gatgatgcgc gccatcgtcg
     6001 tgtcgcccga aagccgcgtc accctcacct cgagggcgcc gtgaccgttc acgctgcccg
     6061 cgaacacccc gtcgccctgc gccttgtcca ccggcatcga ctcgcccgtg atcggcgcct
     6121 ggttcacctc gctcgccccc gccgcgacca ccccgtcgag cgcgatccgt tcccccggcc
     6181 gtaccacgag cgtgtctccg acctccacct cgtcgagcgg cagccggcgc tcgccttcgg
     6241 gccggcgcac cagcgcgtcg gcgggagtca gttccatcag cgagcggatg gcgcggcggg
     6301 cgcgctccat gctgcgggtc tcgagcagct gggcgagcgc gaacaggaag atgacggccg
     6361 cggcctccga ccactcgccg aggacgagcg cgccggccac cgccaccaac atcaggacgt
     6421 tgatgtcgaa cgacgcgctt cgggcagccg cgagggcgcg tcgcgcggtc agcgacgccg
     6481 ccaacgcaat cgacacggcg tacaggagat aggcgagcgg ccgcggcccg ccggccagct
     6541 cggcagccag ccctgcgagg aaggctgccc ccgacgccgc gacgacgaag acgcggcggc
     6601 gcccggtgcc gatgctggcc gccaccggct gctcgtgctc gagccaggcc cgcatcccgg
     6661 tggccgccac cgcctcggca acctgcgacg ccgagagccg ggccgcgtcg tgggcgacgc
     6721 gcaggcgccc gctcaccacg tcggctgaga agcgctcgag gccaggcagg tgccgcaggt
     6781 gccgctccag cagcgccacc tcctcgtggc agtccatccc gtcaacgcgg tagaccgact
     6841 cggtgtggac ctcgcagacc gggcactcgg cgacgttcgc ccggctcacg gccgctccct
     6901 cgccgatgcc tcttccacgt ggcgcaggcc ctcgcggaac agcccggtga tgtgatcgtc
     6961 gtcgagggcg tagaacacca tcctgccctc gcgccgcgag cgcaccagcc tcaagccgcg
     7021 gagaacccgc agctggtgcg acacggccga ctgcgacaag cccagcagcg tggccacgtc
     7081 gcacacgcac aactcggcgt gcgacagcgc gtcgaggatc cgcacgcgcg tcgggtcgcc
     7141 cagcacgcgg aaggtttcgg ccaacagctc gaccgagccg gtcccgacca gcgccgcccg
     7201 cacggcggca acgcgcccgg cgtcggccac gaagacctcg caggcgtcag cggcgtctga
     7261 gttcgaaacg gcccgcctga cgggcgaagc cgcgccggtg gcggttgtga ccatggagtc
     7321 attatatgaa catgtgttca tatattcaat gttgcgattt cagttaaaat cgaaggcttt
     7381 gaacgaccgg tcgactccac cacttggtcc cagggagcac ggtgtgattg aagttcaaca
     7441 cctcaccaaa cgctacggcc gcgtctcggc ggtcgaggac atcagtttcc gggtcgagcg
     7501 cggggagatc ctggggtttc tcggtcccaa cggcgccggc aagaccacga cg
//
LOCUS       DAOPQX010000178         7526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10887_length_7526_cov_5.985678, whole genome shotgun sequence.
ACCESSION   DAOPQX010000178 DAOPQX010000000
VERSION     DAOPQX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10887_length_7526_cov_5.985678"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKK95_10380"
     CDS             <1..581
                     /locus_tag="PKK95_10380"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOC18668.1"
                     /translation="RVSWRGVPVIVAASTETGARGFDFFAPVEAAGALADALSREGAA
                     RLDPAAAEVLRVEAGRPAFGVDMDQDTIPLEAGIEARGISFTKGCYPGQEVVVRILHR
                     GHGRVARRLAGLLIDGDEAPGPGDLLRAGDRDAGRVTSGVFSPALGRPIAMAMLQRDF
                     LEPGTQLTVLHDGTPFPATVTGLPFVQGVGSN"
     gene            complement(661..2079)
                     /gene="uxaC"
                     /locus_tag="PKK95_10385"
     CDS             complement(661..2079)
                     /gene="uxaC"
                     /locus_tag="PKK95_10385"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOC18669.1"
                     /translation="MAPFIHDDFLLGSARARDLFHRVAEPLPIIDYHCHLPAARIATD
                     RPFRSITELWLDGDHYKWRAMRANGIPERLITGEATDWEKFEAWSATVPWTLRNPLYH
                     WTHMELRRPFGIVTLLSPESARVTFDRCNECLRHPGFSPRGLLRQFKVVLVVTTDDPA
                     DSLEHHRALAAHEDTRVYPGWRPDAALGVGDPAAFSAWVDRLEAAANRSIATFGQFLE
                     ALDQRHAAFHDAGCRSSDHGLERIEAEPWSDAEVAATFEAARAGRLVDAGAAARYRSA
                     LLHRFALMDHERGWMQQYHLGALRNTSSRLRQLLGADCGADSIGDFEMARPLARFLDG
                     LDSQGRLARTVLYNLNPRDNELMAAMTGNFQDGSVPGKLQFGSGWWFLDQKDGMEAQI
                     RALSNLGLFSRFIGMLTDSRSFVSYSRHEYFRRLVCDILGRDVEQGLVPDDDVMLERL
                     VRGVCFENARDWLRMPLGVGSN"
     gene            2218..3597
                     /locus_tag="PKK95_10390"
     CDS             2218..3597
                     /locus_tag="PKK95_10390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC18670.1"
                     /translation="MPTPTAPGPSATAAPVKSGGYFRWVICGLLFLATTINYIDRQVI
                     GILKPTLQEEFAWTESDYADIVFAFQLAYAIGMVLAGRLMDRLGTKRGFALALTIWSV
                     AAIAHAEAPVFGQWVAPVLGMFGLGYTASVAGFMAARFALGLGESGNFPASVKTVAEW
                     FPRRERALATGIFNAGTNVGALVTPLVVPWITLSFGWYWAFVATGAVGLLWLAIWLPL
                     YATPDAHRRLKPAELAYIQSDPPDPAKRVPWVGLLPLRQTWAVALAKGLTDPIWWVYL
                     FWIPDFLHRNHGLDLTTIGPPIVVIYLVTDVGSVGGGWLSSALIKRGWTVNAARKTAM
                     LTFALAVVPIVFASQVKSMWLAVALISIAAASHQGWSANVYTLVSDMFPRHAVGSVVG
                     FAGMAGAVGGMFISKVVGKILDATGSYVPIFLMAGFAYLIALAVVQLLVPRLKPVEMG
                     DGGTMGKAQ"
     gene            3633..4889
                     /locus_tag="PKK95_10395"
     CDS             3633..4889
                     /locus_tag="PKK95_10395"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOC18671.1"
                     /translation="MVTGFGSAERAITRDEAHAIVAEAFGSLSIERRRVLAVIPDGTR
                     TAPMPMMFDLLERVVGSRAAELDFLVALGTHQPMSDSQLARLVGRPVSGGRAGRSRIM
                     NHRWDDPSALVTAGVIPAAEVSALTGGLMADPVPVTLNRLLFEYDHLILCGPVFPHEV
                     VGFSGGTKYLVPGVAGPAVIDLTHWIGALLTSSRVIGSGYTPVRAVVDRAAGMVNRPM
                     TCAAMVVEGATLRGLFVGPPREAWENASALSSQVHIVRVPKPLARVLSVMPPMYDDLW
                     TAAKGMYKLEPAVADGGEVVIYAPGVTEVSYTHGALIDEVGYHCRDYFLGQWDRFKHY
                     PGAILAHSTHVKGLGTYDAATGEECPRITVTLATGIPRERCERINLGYLDPAAIDVNE
                     WKGREAEGILVVPRAGEMLYRLTGEA"
     gene            4889..6385
                     /locus_tag="PKK95_10400"
     CDS             4889..6385
                     /locus_tag="PKK95_10400"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOC18672.1"
                     /translation="MNILSANVPGLVARSVVAAAGGEWALAREGAKRRLVALLPRESR
                     PLAELEGDRAERGGRTLVIGPASPRNASALRTFLPWLCPAPIGLRTSAGFGDRLGLAT
                     PGHVRALRAAAPDLAPIFAQQSIREMTRTGRTPVQVLDDATWGAFEEGWTGGVGSDAD
                     HLKAEGEIDACLEAGFTLFTFDPGAFVDAAAASSGREELARALVRVPWDRLETSLQAL
                     AARYVGQPFGIEGVDVSFDEDAVARAAIKYGAAVAHVAALYRHLCERAGSRPFEVEVS
                     VDETEAPTSHAEHVFVATELRRLGVKWVSLAPRFVGRFEKGVDYIGDLEEFGRHLAGH
                     AAIARALGPYKLSLHSGSDKFSLYGAVARETRGLVHLKTAGTSYLEALRVVAAVQPEL
                     FREIFWFACGRYEEDRASYHVSAAAARAAAALEARGEPRAALDDFHVRQVLHVTFGSV
                     LTAADPRGRPRFAHRIAAVLDESPEAYATGLESHFARHLGPFAGCASA"
     gene            6530..7342
                     /locus_tag="PKK95_10405"
     CDS             6530..7342
                     /locus_tag="PKK95_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC18673.1"
                     /translation="MFDLSGRTIAITGALGVLGCAISRGLAASGANLALLDHRIASGQ
                     KFCASLGEEGSSRTVVIEADVLNPESLARAADAVVHRFGQVDGLINAAGGNRPEATTG
                     PGRTFFDLPADALLKTLDLNLIGTILPCQAFGRHMAARGEGVILNVSSMNAFRPLTRI
                     PAYSAAKAAVSNFTQWLAVHMAQELSPRIRVNAIAPGFFLTEQNRFLLTDAETGALTA
                     RGQSILSHTPMARFGDPDDLLGAVIWLMSPASAFVTGIVVPIDGGFSAFSGV"
BASE COUNT         1032 a         2590 c         2738 g         1166 t
ORIGIN      
        1 cccgggtttc ctggcgaggc gtgccggtca tcgtggcggc atccaccgag acgggcgccc
       61 gcgggttcga tttcttcgcg ccggtcgagg cggccggcgc gctggccgac gctttgtctc
      121 gggagggcgc ggcccggctc gacccggccg ccgccgaggt gctgcgggtc gaggccggcc
      181 ggccggcgtt cggcgtggac atggaccagg acacgatccc gctcgaagcg gggatcgagg
      241 cgagggggat cagcttcacg aaggggtgct acccggggca ggaggtggtc gtccggatcc
      301 tccaccgcgg ccacggccgc gtggcccgca ggctggcggg gctgctgatc gatggcgacg
      361 aggcgcccgg gcccggcgac ctgctgcggg cgggggaccg cgacgccggc cgcgtgacca
      421 gcggggtgtt ttcccccgcg ctgggccggc cgatcgcgat ggcgatgctg cagcgcgatt
      481 tcctcgagcc cggcacgcag ctgaccgtcc ttcacgacgg gacgccgttc ccggcgacgg
      541 tgaccggcct gcccttcgtc caaggggtcg ggagtaatta gaagtcgtgc cggactcccg
      601 gcggagaggt cgagagtact tagtcgagag cacctagatg acgaaaatcc ggcaggactt
      661 ctaattactc ccgaccccga gcggcatccg aagccagtcg cgagcgttct cgaagcacac
      721 gccccgcacc agccgctcga gcatcacgtc gtcgtcgggc acgaggccct gctcgacgtc
      781 gcgcccgagg atgtcgcaga ccagccgcct gaagtactcg tgacgcgagt acgacacgaa
      841 gctccgcgag tcggtcagca tcccgatgaa gcgcgagaag aggccgaggt tcgagagcgc
      901 gcggatctgg gcctccatcc cgtccttctg atccaggaac caccagcccg agccgaactg
      961 cagcttcccc ggcaccgatc cgtcctggaa gttgccggtc atcgccgcca tcagctcgtt
     1021 gtcgcggggg ttgaggttgt agaggaccgt gcgggccagc cgcccctggc tgtcgagacc
     1081 gtcgaggaag cgggccaacg gtctggccat ctcgaagtcg ccgatggagt cggcgccgca
     1141 gtcggcgccg agcagctgcc gcaggcggga cgaggtgttg cgcagcgcgc cgaggtggta
     1201 ctgctgcatc cacccgcgct cgtgatccat gagagcgaag cggtggagca gcgccgaacg
     1261 gtagcgcgcc gccgccccgg catcgacgag gcgtccggcc cgcgccgcct cgaaggtggc
     1321 ggcgacctcg gcgtccgacc acggctcggc ctcgatccgc tcgagcccgt gatccgacga
     1381 acggcacccc gcgtcgtgga aggcggcgtg gcgctggtcg agcgcctcga ggaactggcc
     1441 gaacgtcgcg atcgagcggt tcgccgccgc ctcgagccgg tccacccacg cgctgaaggc
     1501 ggccgggtcg ccgaccccga gcgcggcgtc gggccgccag ccggggtaca cgcgggtatc
     1561 ctcgtgggcg gcgagcgcgc ggtgatgttc gagcgagtcg gccgggtcgt cggtggtgac
     1621 gaccagcacc accttgaact ggcggagcag gccgcggggc gagaagccgg ggtggcgcag
     1681 gcactcgttg cagcggtcga aggtgacgcg cgcgctctcg ggcgagagca gcgtcacgat
     1741 gccgaacggg cgccgcagct ccatgtgggt ccagtggtag agcgggttgc gcagcgtcca
     1801 ggggacggtg gcggaccacg cctcgaactt ctcccagtcg gtggcttcgc cggtgatcag
     1861 gcgctcggga atgccgttgg cccgcatcgc ccgccacttg tagtggtcac cgtcgagcca
     1921 cagctcggtg atcgaacgga aggggcgatc cgtggcgatc cgggccgcgg gcaggtggca
     1981 gtggtagtcg atgatcggca gcggttcggc gacccggtgg aagaggtcgc gggccctggc
     2041 cgagccgagc aggaagtcgt cgtggatgaa gggcgccatc ggtgtcccga tcgtcgaaat
     2101 tgaccccgcg gtgggaattc gttataccac ttccgcgccc gggtctccac gcggcccggc
     2161 tgaggaggcc ttcgatgtcg ctcggggcgc cccgagcgca tcgaggaagg agtgcatgtg
     2221 cccacgccga ccgcgcccgg gccgtccgcg acggccgcac ccgtcaagtc cggcggctac
     2281 ttccgctggg tgatctgcgg gctgctcttc ctggcgacca cgatcaacta catcgaccgg
     2341 caggtcatcg gcatcctgaa gccgaccctg caggaggagt tcgcctggac cgagtccgac
     2401 tacgccgaca tcgtcttcgc cttccagctc gcgtacgcga tcgggatggt gctggcggga
     2461 cggctgatgg accgcctcgg caccaagcgg ggcttcgccc tcgcgctcac catctggagc
     2521 gtggccgcca tcgcgcacgc cgaggcgccg gtcttcggcc agtgggtggc gcccgttctc
     2581 ggcatgttcg gcctcggcta cacggcctcg gtggccggct tcatggccgc ccgtttcgcg
     2641 ctcggcctcg gcgagtcggg caacttcccc gcgtcggtga agacggtggc ggagtggttc
     2701 ccgcggcggg agcgggcgct ggccaccggc atcttcaacg cgggcaccaa cgtgggggcc
     2761 ctcgtcacgc cgctggtggt gccctggatc accctctcct ttggatggta ctgggcgttt
     2821 gtcgcgaccg gcgccgtcgg ccttctctgg ctcgccatct ggctgccgct ctacgcgacg
     2881 cccgatgccc accggcgcct gaagcccgcc gagctggcct acatccagag tgacccgccc
     2941 gacccggcca agcgggtgcc gtgggtcggg ctgctgccgc tgcgccagac ctgggcggtg
     3001 gccctcgcca aggggctgac cgatccgatc tggtgggtgt accttttctg gatacccgac
     3061 ttcctccacc gcaaccacgg gctcgacctc accacgatcg gccctcccat cgtcgtgatc
     3121 tacctcgtga ccgacgtcgg gagcgtcggg ggcgggtggc tgtcgtcggc gctcatcaag
     3181 cgaggctgga cggtgaacgc cgcccgcaag acggcgatgc tgaccttcgc gctcgcggtc
     3241 gtgccgatcg tcttcgcctc gcaggtgaag agcatgtggc tcgcggtcgc gctgatcagc
     3301 attgccgccg cgtcgcacca ggggtggtcg gccaacgtct acacgctggt ctccgacatg
     3361 ttcccccgcc atgccgtcgg atcggtggtc ggcttcgcgg gaatggccgg ggcggtcggg
     3421 ggcatgttca tctcgaaggt cgtggggaag atcctcgacg cgaccggcag ctacgttccg
     3481 atcttcctga tggcggggtt cgcctacctg atcgcgctcg cggtggttca gctgctggtg
     3541 ccgcggctga agcccgtgga gatgggagac ggggggacga tgggaaaggc acaatgaaga
     3601 atgcagaatg tagaaaggcg gaggctttct gaatggtgac cggattcggg tcggctgagc
     3661 gggcgatcac gcgcgacgag gcgcacgcaa tcgtggccga ggcgttcggc tcgctgtcga
     3721 tcgagcggcg gcgcgtgctc gcggtgatcc ccgacgggac acggaccgcg ccgatgccga
     3781 tgatgttcga cctgctcgag cgggtcgtcg gctcgcgggc ggcggagctc gacttcctgg
     3841 ttgcgctcgg cacgcaccag ccgatgtccg attcgcagct ggccaggctg gtcgggcggc
     3901 cggtctcggg cgggcgggcc gggcgcagcc ggatcatgaa ccaccggtgg gacgacccgt
     3961 cggcgctcgt cactgccggc gtcatccccg ccgccgaggt gtccgcactc acgggcggct
     4021 tgatggccga ccccgtcccg gtgacgctga accggctgct cttcgagtac gaccacctga
     4081 tcctgtgcgg cccggtgttc ccgcacgagg tcgtcggttt ctcgggcggg accaagtacc
     4141 tggtgccggg cgttgccggg cccgccgtca tcgatctcac gcactggatc ggcgcgctgc
     4201 tcacctcgag ccgcgtgatc ggctcaggct acactccggt ccgcgccgtc gtcgatcgtg
     4261 ccgcggggat ggtgaaccgg ccgatgacct gcgcggccat ggtggtggag ggggccacgc
     4321 tgagggggct cttcgtgggg ccgcctcgag aggcgtggga gaatgcctcg gccctctcct
     4381 cgcaggtcca catcgtccgc gtgccgaagc ccctcgcgcg cgtgctgtcg gtcatgccgc
     4441 cgatgtacga cgacctgtgg acggcggcca aggggatgta caagctcgag ccggccgtcg
     4501 ccgacggcgg cgaggtggtg atctacgcgc cgggcgtgac cgaggtgagc tacacccacg
     4561 gcgcgctgat cgacgaggtc ggctatcact gccgcgacta cttcctcggc cagtgggacc
     4621 gattcaagca ctaccccggt gccatcctcg cccactcgac gcacgtcaag gggcttggca
     4681 cgtacgacgc cgccaccggg gaggagtgcc cgcggatcac cgtgacgctc gcgacgggca
     4741 tcccgaggga gcggtgcgag cgcatcaacc tgggctacct cgacccggcg gcaatcgacg
     4801 tgaacgagtg gaaagggcgc gaggccgaag gcatcctggt ggtgccgcgc gccggtgaga
     4861 tgctctaccg gctgaccgga gaggcgtgat gaacatcctg tcggcgaacg tgcccgggct
     4921 cgtcgcccgg tcggtcgttg cggcggccgg gggcgagtgg gccctggcca gggagggcgc
     4981 gaagcgccgc ctcgtcgcgc tgctcccgcg cgagtcgcgg ccgctcgcgg agctggaggg
     5041 cgaccgcgcc gagcgcgggg ggcggacgct ggtgatcggc ccggcgagcc ctcgcaacgc
     5101 gagcgcgctc cgcacgttcc ttccctggct ttgtcccgcg ccgatcggcc tgcgcacctc
     5161 ggcgggtttc ggagaccggc tgggcctcgc caccccgggg cacgtccggg cgctgcgagc
     5221 cgcggccccc gacctggcgc cgatcttcgc gcagcagtcg atccgcgaga tgacccggac
     5281 gggccgcacg ccggtgcagg tgctggacga tgcgacgtgg ggcgcgttcg aggagggctg
     5341 gacgggcggc gtcgggtcgg atgccgatca cctgaaggcc gagggggaga tcgacgcctg
     5401 cctcgaggcc gggttcaccc tcttcacgtt cgatcccggc gcgttcgtgg acgccgcggc
     5461 ggcatcgtcc ggccgcgagg agctggcgcg ggcactcgtc cgggtgccgt gggatcggct
     5521 cgaaacgtcg ctccaggcgc tggcggccag gtacgtcggg cagccgttcg gcatcgaggg
     5581 tgttgacgtc tccttcgacg aggacgcggt tgcgcgcgcg gcgatcaagt acggggcggc
     5641 cgtggcacac gtggcggccc tctaccgcca cctgtgcgag cgcgccggca gccggccgtt
     5701 cgaggtggag gtgtcggtcg acgagaccga ggccccgacc tcgcacgccg agcacgtgtt
     5761 cgtggcgacc gagctgcgcc ggctcggggt gaagtgggtg agccttgccc cccggttcgt
     5821 cggccggttc gaaaagggcg tggactacat cggcgacctg gaggagttcg gccggcacct
     5881 cgccggccac gccgccatcg cgcgcgccct cggaccctac aagctgagcc tccattcggg
     5941 ctccgacaag ttcagcctct acggcgcggt cgcacgcgag acgcgcggcc tcgttcacct
     6001 gaagaccgcg ggcaccagct acctcgaggc gctgcgggtc gtcgccgcgg tgcagcctga
     6061 gctgttccgc gagattttct ggttcgcctg cggccgctac gaggaggacc gcgccagcta
     6121 ccacgtgtcg gccgccgcgg cgcgcgccgc cgcggcgctc gaggcccgcg gcgagccgag
     6181 ggcggcgctc gacgacttcc acgtgcggca ggtgctgcac gtcacgttcg gctcggtgct
     6241 gacggcggcc gatccgcgcg gccggccccg attcgcgcac cggatcgccg cggtgctgga
     6301 cgagtctccc gaggcctacg ccaccggcct cgagagccac ttcgcgcggc acctcggccc
     6361 gttcgccggc tgcgccagcg cgtagccggc cccccgcccc cgcgggcacc ccgatccgat
     6421 cccctgctcc ctgccccccg atccctgctc cctgttccct gatccctgat ccctgatagc
     6481 atctgcccag gtgataccta cagagatgac ccaaccgtca agagccagcg tgttcgacct
     6541 gtccggacgc accatcgcca tcaccggcgc cctcggcgtg ctcgggtgcg caatctcgag
     6601 gggccttgcg gccagcgggg ccaacctcgc cctgctcgac caccgcatcg cgagcggcca
     6661 gaagttctgc gcgtcgctcg gcgaggaggg gtcctcgcgc acggtggtca tcgaggccga
     6721 cgtgctcaat cccgagagcc tggcccgcgc cgcagacgcg gtcgtccatc gcttcggcca
     6781 ggtggacggg ctgatcaacg cggccggcgg gaatcggccc gaggcgacga cggggcccgg
     6841 gcgcaccttc ttcgacctgc cggccgacgc cttgttgaag acgctggatc tcaacctgat
     6901 cggcaccatc cttccgtgcc aggcgttcgg ccgccacatg gcggcgcggg gcgagggggt
     6961 catcctgaac gtctcgtcga tgaacgcgtt ccggccgctg acgcgcatcc ccgcctactc
     7021 ggccgcgaag gcggcggtca gcaacttcac ccagtggctg gccgtgcaca tggcgcagga
     7081 gttgtcgccc cgcatccggg tgaacgcgat tgcgccgggg ttcttcctca ccgagcagaa
     7141 ccggttcctg ttgaccgacg cggagaccgg cgcgttgacc gcgcgcggcc agagcatcct
     7201 ctcgcacacg ccgatggcgc ggttcggcga tccggatgac ctgctagggg ccgtgatctg
     7261 gctgatgtct ccggcctcgg cgtttgtgac cggcatcgtg gtgccgatcg acggcggctt
     7321 ctccgcgttc agcggcgttt gacctggagt tcacgacatg gagttccaca ttggagtcgt
     7381 cggcctgggc gtgatggggg cgaacctggc cctcaacatc gagcggaacg ggttcccggt
     7441 ggtcggctac gacgtctcgg cggcccagac gaaggcgttc ctcgacggcc cggtcgagca
     7501 gctctgcgcg atgctcgacg actggc
//
LOCUS       DAOPQX010000179         7493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_10955_length_7493_cov_4.858430, whole genome shotgun sequence.
ACCESSION   DAOPQX010000179 DAOPQX010000000
VERSION     DAOPQX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10955_length_7493_cov_4.858430"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2754)
                     /locus_tag="PKK95_10410"
     CDS             complement(<1..2754)
                     /locus_tag="PKK95_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC18674.1"
                     /translation="MSIPRTAIHRPVMMFMICAVIVLLGAISLTRLPIDLLPEISYPS
                     ITIRVGYEGVGPLEMEELVTRPIEQAVAAVSGLERINATSSEGNSTVRLNFAWGTDLN
                     EAADDVRTRIDRVKGRLPEDADVPTIFKFDSSTMPIMSIGVEGDYDRVTLREIAEREL
                     SPRMERVAGVAAVTAGGGLRRQIHVELSKEKIAALNLSVNRVISQLRTENQNTPLGLI
                     DEGDMTYLLRSQGQFENLEQIRNIVVFTRDNVPVYLRDIAEVKDSTEDPRSLMRINGR
                     PGVRMQVTKQSGKNTVAVAAAMRQEIERINASMPGIRLLILDDSSTYIEDSIASVRES
                     ALLGALFVVVVIFAFLRNVRSTLIIGISIPISIIGTFALLYFSGYTLNTMTFGGLALG
                     VGMIVDASIVVLENAFRHMEEKKKDRFTAAIEGSEEVWSAILASILTHVAVFVPLLFL
                     SGVSNIMFKQLAVVVMFSLLMSLFVAVTIVPVLCSRLLVLPKPLDQRRGVTGRMFTAT
                     ERALAWLDNLYRGTLDKALHHRPTVIAGGAALFLVALLLLPTIPFELMPETDEGQVRV
                     DVELPVGTRIERTEAIVLRLEQMIEKLVPEKIDLMSSAGGGGWMGGSTHRGDLTIRLV
                     PKDERKRSSDQIAMALRRELSGLPGVIIRTRASGGNRQMTRMLSGGGGDSRLSFEIRG
                     HDLEDARALGVRAKAVLDRTPGVADSRIGREEGRPELAVRVDRQKAALLGLGLAEVAN
                     TIQTNIQGTTAAYYRERGFEYPIVVRLRAEDRERSVSVDDVRVTNAAGQVVQAKNLID
                     IGTQLGPVQIERKNQERILYVNAELETALSEAMERVNAQVPQMNVPRGFSVGFGAEAE
                     EQAKAFNQLQMVLILAIVLVYAVMASQYESLRDPFIILFSIPLAAIGVVAALKLTSTP
                     FSMQ"
     gene            complement(3035..4291)
                     /locus_tag="PKK95_10415"
     CDS             complement(3035..4291)
                     /locus_tag="PKK95_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC18675.1"
                     /translation="MRKTLCTIGLVAALAGGLTFYGCGGDQAAGQPGARPGGGPPGMG
                     GGAGMNMPMTVELAATSRAPLNETIVVVGNLIGAATVDVVPKVSGRLQSVQVRLGDPV
                     RKGQALAKVEDSELREQVRQAEASYKVSEATIRQREADLNLSKTNLERSRSLFERNLV
                     PRQTLDDTEAQHQAALAQLDLARAQFEQSKARLDELRINLSNTVIASPVDGFVGRRNV
                     DAGAFVSQNVPVASVVDIHLVRLVVNLVERDLSRVSAGMPTEVGVDAFPGEVFKGKVA
                     RVAPVLDPATRTAQMEVEVMNPDFRLKPGMYARVQITVDRREKALVVPRNAIVDVEGK
                     RGVFLAADSRAQFRPVQTGLQDAELVEIVAGLSDGEKVITTGAAGLKDGDVIVLAGQA
                     PAGRPAQGQGGPRGAGAAPAANQAAR"
     gene            complement(4357..6111)
                     /locus_tag="PKK95_10420"
     CDS             complement(4357..6111)
                     /locus_tag="PKK95_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC18676.1"
                     /translation="MKKSFIQFSTLAAALALVAASPALGQEVSDEKVRSLLAEARVQV
                     QAQPAPAQPAAQAAAGDTRPVLNLTMDEAVARALDHNIDLSVARLNPQLQDLSLQQTR
                     AAYTPTLANTTLGLRGSTSRSTNTLFGGNLVDQKVMTYNTGITQVLPWTGGSFSLGFN
                     NTRQNTTNKNQTVNPLYNTGLTLNFSQPLLRNLTIDNTRQSISTGIINRRLADVTLRG
                     TTISTSASTRNAYWDLVYAYQAVEAAQRSLALAEKLVQDNQARVEIGTMAPIDVVQAQ
                     SEVATRQLALVQAEATLRTSELALKRLLVDSTADPLWTSRIVPTDRPAADTGERVDVE
                     GALKRALGERTDIIQAREQLAINDISLRYLKNQKLPDVTLNAQYGAAGIGGTQIVRSD
                     RIDGDILETFPGGYNDALSELRRWRLPSWNVNLTVSYPLGTSAQDVAHARAQVQLKQT
                     QAQLRSLELRVATEITSAALTVQNNLQQVQAAAASRELAQRRLEAEQSKFEVGMSTNY
                     NVVLAQRDFADAQDAELRALLNYRKSLVEFERLQQTGGSSGVTSVSTSGASGGSGGSS
                     GGAGGGFSGGGGGGFSDR"
     gene            complement(6152..6595)
                     /locus_tag="PKK95_10425"
     CDS             complement(6152..6595)
                     /locus_tag="PKK95_10425"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOC18677.1"
                     /translation="MSKRVVAILALLGWVAGAAGAASADPQSRAPWWKDESSKAELRL
                     TDDQATRIDAVFRESWERMKSSYEELGRREKALSALVSGPDTTEADVVRQVEQVEAVR
                     GELNKIRTVMLYRMHRILTPEQRVKLDEVRRARERDRERSRREPR"
     gene            complement(6651..>7493)
                     /locus_tag="PKK95_10430"
     CDS             complement(6651..>7493)
                     /locus_tag="PKK95_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18678.1"
                     /translation="PGDVVILPAFGVTIAMLEQLQRQGCTLVDTTCGSVLNVWKNVRR
                     YAQDGFTSIIHGKYKHEETRATASQALQFPNGRYLVVLDRAQTRTVCDYIRDGGDREA
                     FRGQFAPAASDGFDPDHDLGRVGLANQTTMLMSESVEIAGMLRQAIADRYGESELPAR
                     FRAFETICSATQDRQDAVIELLDRQALDLMIVIGGYNSSNTCNLARICAARLPTYHIA
                     GVDALLSAGAVRHRRVDGVEVVDEGWLPPDRPLAIGITAGASTPNAIVGQVIERLGAL
                     ANAG"
BASE COUNT         1086 a         2663 c         2538 g         1206 t
ORIGIN      
        1 ctgcatgctg aacggcgttg acgtcagctt gagcgcggcc accacgccga tggcggccag
       61 cgggatcgag aagaggatga tgaacgggtc gcgcagcgat tcgtactgcg aggccatgac
      121 ggcgtagacg aggacgatcg ccaggatcag gaccatctgc agctggttga aggccttcgc
      181 ctgctcctcg gcctcggcgc cgaagcccac cgagaagccg cgcggcacgt tcatctgcgg
      241 cacctgcgcg ttgacccgct ccatcgcctc gctcagcgcg gtctccagct cggcgttgac
      301 gtacaggatc cgctcctggt tcttgcgctc gatctggacg gggccgagct gggtgccgat
      361 gtcgatcagg ttcttcgcct gcacgacctg cccggcggcg ttggttaccc ggacgtcgtc
      421 caccgacacc gagcgctcgc ggtcctcggc ccgcaggcgg acgacgatcg ggtactcgaa
      481 cccgcgctcg cggtagtagg ccgcggtcgt cccctggatg ttcgtctgga tcgtgttggc
      541 gacctcggcg agcccgaggc ccagcagcgc cgccttctgg cggtcgacgc ggacggccag
      601 ctcggggcgc ccctcctcgc ggccgatccg cgagtcggca acgcctggcg tcctgtccag
      661 caccgccttg gcgcgaacgc cgagcgcgcg cgcgtcctcg aggtcgtggc cgcggatctc
      721 gaacgagagg cgcgagtcgc cgccgccgcc gctcagcatc cgggtcatct ggcggttgcc
      781 gcccgaggcc cgggtccgga tgatcacacc gggcagcccc gagagctcgc gccgcagcgc
      841 catggcgatc tggtcgctcg accgcttgcg ctcgtccttg ggaacgagcc ggatggtgag
      901 atcgccccgg tgggtcgagc cgcccatcca cccgccgccg cccgccgagc tcatcagatc
      961 gatcttctcg ggcacgagct tctcgatcat ctgctcgagc cggaggacga tcgcctcggt
     1021 gcgctcgatc cgggtgccga ccggcagctc cacgtccacg cggacctggc cctcgtcggt
     1081 ctcgggcatc agctcgaagg ggatcgtggg cagcagcagg agggcgacca ggaacagggc
     1141 cgcgccgccc gcgatgacgg tggggcggtg gtgcagcgcc ttgtccagcg tcccgcggta
     1201 gaggttgtcg agccaggcca gcgcgcgctc ggtcgcggtg aacattcggc cggtcacccc
     1261 gcgccgctgg tcgagcggct tcggcagcac caggaggcgc gagcagagga ccggcacgat
     1321 ggtcaccgcg acgaacagcg acatcagcag cgagaacatc acgacgacgg ccagctgctt
     1381 gaacatgatg ttcgagacgc cgctgaggaa cagcagcggc acgaacaccg cgacgtgcgt
     1441 caggatcgac gcgaggatgg ccgaccacac ctcctcgctc ccctcgatcg cggccgtgaa
     1501 gcgatccttc ttcttctcct ccatgtggcg gaacgcgttc tcgaggacga cgatcgaggc
     1561 gtccacgatc atcccgactc ccagcgcgag gccgccgaag gtcatcgtgt tgagcgtgta
     1621 cccggagaag taaaggagcg cgaaggtgcc gatgatcgag atcgggatcg agatcccgat
     1681 gatcaacgtc gacctcacgt tgcgcaggaa cgcgaagatg accaccacga cgaagagcgc
     1741 accgagcagc gccgactcgc gcaccgaggc gatcgagtcc tcgatgtagg tcgagctgtc
     1801 atcgaggatc agcagccgga tgccaggcat cgacgcgttg atccgctcga tctcctggcg
     1861 catcgccgcc gcgaccgcca ccgtgttctt gcccgactgc ttggtgacct gcatccgcac
     1921 gcccggccgc ccgttgatcc gcatcagcga gcgcgggtcc tcggtcgagt ccttgacctc
     1981 ggcgatgtcg cgcaggtaga ccggcacgtt gtcccgggtg aagaccacga tgttgcggat
     2041 ctgctccaga ttctcgaact gcccctggct gcggagcagg taggtcatgt cgccctcgtc
     2101 gatcagcccg agcggcgtgt tctggttctc ggtccgcagc tggctgatga cgcggttcac
     2161 cgacaggttg agggccgcga tcttctcctt cgacagctcg acgtggatct gccggcgcag
     2221 cccgccgccc gccgtgacgg cggcgactcc cgccacgcgc tccatccgcg gcgacagctc
     2281 gcgctcggcg atctcgcgca gcgtcacccg gtcgtagtcg ccctcgacgc cgatgctcat
     2341 aatcggcatg gtcgacgagt cgaacttgaa gatcgtcggg acatccgcgt cctcggggag
     2401 ccgccccttg acccggtcga tgcgcgtccg gacgtcgtcg gccgcctcgt tcaggtcggt
     2461 gccccaggcg aagttcaggc ggaccgtgct gttgccttcc gaggaggtgg cgttgatccg
     2521 ctcgaggccg gaaacggcgg cgaccgcctg ctcgatcggc cgcgtgacca gctcctccat
     2581 ctccagcggg ccgacaccct cgtacccgac ccggatggtg atgctcgggt aggagatctc
     2641 gggcagcagg tcgatcggca gccgggtgag cgagatggcg ccgagaagga cgatcaccgc
     2701 gcagatcatg aacatcatga ccgggcggtg gatggcggtg cgggggatgc tcatagacgg
     2761 aatcccaatc ccaatcccaa tcccaatccc aatcccaatc ccaatcccaa tcggcgcgtc
     2821 ccgggcgacg ggccgatgcc tcttacagcg tgcaccccga ggtgccgtcc agcagggtgc
     2881 tcacgaggag ctcgtgtaac ggagaacatc gaacggagaa cggagaatgc atggccattc
     2941 tccgttttcc gtttgtcgtt ttccgtggtt cgacgagctc accacgcccc gagcgcagtc
     3001 gaggggcgtt caggccgcct tcctcagcgg cctcctagcg cgcggcctgg ttggcggccg
     3061 gggccgcccc cgcgccgcgc gggccgccct gtccctgggc gggccggccg gctggagcct
     3121 ggcccgcgag cacgatcacg tcgccgtcct tgaggccggc cgcgccggtc gtgatgacct
     3181 tctccccgtc cgagagcccc gccacgatct ccaccagctc ggcatcctgc aggccggtct
     3241 ggacggggcg gaactgggcg cgggagtcgg cggcgaggaa caccccccgc ttgccctcga
     3301 cgtccacgat ggcgttgcgc ggcaccacca gcgccttctc gcgacggtcc acggtgatct
     3361 gcacgcgcgc gtacatcccg ggcttcagcc ggaagtcggg gttcatcacc tcgacctcca
     3421 tctgcgccgt ccgcgtcgcg gggtcgagga cgggcgccac gcgcgccacc ttgcccttga
     3481 agacctcccc cgggaacgcg tcgacgccca cctcggtcgg catgccggcg ctcacccggc
     3541 tcaggtctct ctcgaccagg ttcacgacca gccgcacgag gtggatgtcc accaccgacg
     3601 ccaccggcac gttctgcgac acgaacgccc ccgcgtccac gttgcgccgg ccgacgaagc
     3661 cgtccaccgg cgaggcaatc acggtgttcg acaggttgat gcgcagctcg tcgaggcgcg
     3721 ccttcgactg ctcgaactgc gcccgggcga gatcgagctg ggcgagcgcg gcctggtgct
     3781 gcgcctccgt gtcgtcgagc gtctggcgcg ggaccaggtt gcgctcgaac aggctgcggg
     3841 aacgctcgag gttggtcttc gacaggttga ggtcggcctc gcgctgccgg atggtcgcct
     3901 cggacacctt gtaggaggcc tcggcctggc ggacctgctc ccgcagctca gagtcttcga
     3961 ccttggcgag ggcctggccc ttgcgcaccg ggtcgcccag gcgcacctgc accgactgca
     4021 ggcggccgct gaccttcggc acgacatcca cggtggccgc cccgatcagg ttgcccacca
     4081 cgacgatcgt ctcgttgagc ggcgcgcgcg aggtggccgc cagctcgacg gtcatcggca
     4141 tgttcatccc ggccccgccg cccatccccg gcgggccgcc gcccggcctc gcgcccggct
     4201 gacccgcggc ctggtcgccg ccgcagccgt agaacgtcag gccgcccgcc agcgcggcga
     4261 ccagccctat cgtgcaaagt gttttacgca taactcgacg ctgcctaccc gacagaaaac
     4321 tggaaaagaa ggctgccggc accggcggcc cgcccgctac cgatcgctga aaccgcctcc
     4381 gccgccgccg ctgaagcccc cgcccgcgcc gccggagctg ccgccgctgc cgccggacgc
     4441 gcccgaggtg ctcaccgagg tcacgccgct gctgccgccc gtctgctgca gccgctcgaa
     4501 ctcgaccagc gacttgcggt agttgagcag cgcgcgcagc tcggcgtcct gcgcgtcggc
     4561 gaaatcgcgc tgcgccagaa cgacgttgta gttggtggac atgcccacct cgaacttgct
     4621 ctgctcggcc tcgaggcgcc gctgcgccag ctcgcgcgag gccgccgccg cctgcacctg
     4681 ctgcaggttg ttctgcacgg tcagcgcggc gctggtgatc tcggtcgcca cgcgcagctc
     4741 gagcgaccgg agctgcgcct gcgtctgctt cagctgcacc tgcgcccgcg cgtgcgccac
     4801 gtcctgcgcg ctggtgccga gcgggtagct gacggtgagg ttcacgttcc aggaggggag
     4861 cctccaccgc cgcagctccg agagcgcgtc gttgtagccg cccgggaacg tctcgaggat
     4921 gtcgccgtcg atcctgtcgg agcgcacgat ctgggtgccg ccaatgccgg cggcgccgta
     4981 ctgcgcgttg agcgtgacgt ccggcagctt ctggttcttc aggtagcgga gcgagatgtc
     5041 gttgatcgcc aactgctcgc gcgcctggat gatgtcggtc cgctcgccga gcgcgcgctt
     5101 gagcgccccc tcgacgtcca cccgctcacc cgtgtcggcc gcgggacggt cggtcggaac
     5161 gattcgggag gtccacagcg ggtcggccgt gctgtccacc agcagccgct tcagcgccag
     5221 ctcgctcgtc cgcagcgtcg cctccgcctg gaccagcgcc agctgccgcg tggccacctc
     5281 cgactgcgcc tgcaccacgt cgatcggcgc catcgtgccg atctcaaccc gcgcctggtt
     5341 gtcctgcacg agcttctcgg ccagcgccag cgaccgctgc gccgcctcga ctgcttggta
     5401 cgcgtaaacc aggtcccagt aggcgttgcg cgtgctcgcc gacgtgctga tggtcgtccc
     5461 ccgcagcgtg acgtcggcca ggcggcggtt gatgatgccg gtggagatgg actgacgggt
     5521 gttgtcgatg gtgaggttcc gcagcaaggg ctgggagaag ttcagcgtca accccgtgtt
     5581 gtacaggggg ttgacggtct ggttcttgtt ggtcgtgttc tgccgcgtgt tgttgaagcc
     5641 caggctgaaa ctcccgcccg tccagggaag gacctgggtg atacccgtgt tgtacgtcat
     5701 caccttctgg tccacgaggt tgccgccgaa cagggtgttg gtcgagcggc tcgtgctgcc
     5761 gcgcaggccg agcgtcgtgt tggccagcgt gggcgtgtag gccgcccgcg tctgctgcag
     5821 tgacaggtcc tgcagctggg ggttgagcct ggcgacgctc aaatcgatgt tgtggtcgag
     5881 ggcgcgcgcc accgcctcgt ccatcgtcag gttgaggacg gggcgcgtgt cgccggccgc
     5941 ggcctgcgcc gccggctggg cgggcgccgg ctgggcctgc acctggaccc tggcctcggc
     6001 cagcaggctg cgcaccttct cgtccgaaac ctcctggccg agggcgggcg aggccgcgac
     6061 gagcgccagg gcggccgcga gcgtgctgaa ctggatgaac gacttcttca cgttgcctcc
     6121 acgtacacga aaatccggaa cggcccggcg gctaccgcgg ctcgcggcgt gagcgctcgc
     6181 gatcgcgctc gcgggcgcgg cgcacctcgt ccagcttgac ccgctgctcg ggcgtgagga
     6241 tccggtgcat ccggtagagc atcacggtgc ggatcttgtt cagctccccg cgcacggcct
     6301 cgacctgctc cacctggcgg accacgtcgg cctcggtggt gtcgggcccg ctgaccagcg
     6361 ccgagagggc cttctcgcgc cggcccagct cctcgtagct cgacttcatc cgctcccacg
     6421 actccctgaa gaccgcgtcg atgcgcgtcg cctggtcgtc ggtcagccga agctcggcct
     6481 tcgacgactc gtccttccac cagggagccc gggactgggg gtcggccgag gccgcgccgg
     6541 cggcgcccgc cacccacccg agcagggcca ggattgccac gacgcgtttg gacacagtaa
     6601 gagacactct atgacctcgg aacggattac gacctggggg aaagggacgt ttacccggcg
     6661 ttggcgaggg cgcccagccg ctcgatcacc tggccgacga tcgcgttggg ggtcgaggcc
     6721 cccgccgtga tcccgatcgc gagcgggcga tctggcggca gccagccctc gtccaccacc
     6781 tcgacaccat ccacgcgccg gtggcgcacc gcgccggccg acaggagggc gtccacgccc
     6841 gcgatgtggt aggtgggcag gcgcgcggcg cagatgcgcg ccaggttgca ggtattgctg
     6901 ctgttgtagc ccccgatcac gatcatcagg tcgagcgcct gccggtccag cagctcgatg
     6961 acggcgtcct gccggtcctg cgtggcgctg cagatcgtct cgaaggcgcg gaagcgcgcc
     7021 ggcagctccg actcgccgta tcgatcggcg atcgcctggc gcagcatccc ggcgatctcc
     7081 accgactcgg acatcagcat cgtcgtctgg ttggcgaggc cgacccggcc caggtcgtgg
     7141 tcggggtcga atccatccga tgcagccggc gcgaactgcc cccggaacgc ctcgcggtcg
     7201 cccccgtcac ggatgtagtc gcagacggtg cgcgtctggg cgcgatcgag caccaccagg
     7261 tagcggccgt tcgggaactg gagcgcctgc gacgcggtcg cccgcgtctc ctcgtgcttg
     7321 tacttcccgt ggatgatcga ggtgaagccg tcctgggcgt accgcctgac gttcttccag
     7381 acgttcaaca ccgacccgca ggtggtgtcc accagcgtgc acccctggcg ctgcagctgc
     7441 tcgagcatcg cgatggtgac gccgaaggcc ggcaggatga cgacgtcgcc cgg
//
LOCUS       DAOPQX010000180         7397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11128_length_7397_cov_4.617815, whole genome shotgun sequence.
ACCESSION   DAOPQX010000180 DAOPQX010000000
VERSION     DAOPQX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11128_length_7397_cov_4.617815"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3034
                     /locus_tag="PKK95_10435"
     CDS             <1..3034
                     /locus_tag="PKK95_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HOC18679.1"
                     /translation="LFAGSVGGDGSGGEVTVTQAGSVTVAGDGANGIFAQSAGGRSTG
                     GRVSVALNSGSILATGAGSNGIFAQSIGGGGSGDVSVTIASAASLVVGGSNGGSAVRF
                     AHGNRNTLTNYGTLTTVGGISGLAVVGGTGNETVDNHGTITGSVNLGGGVNTLTNRAE
                     GLLNAGSSIDVGAGGEFANAGTLSLGGLGRAQTTAVTGNFTQSGTPTWLVDIRHPGTS
                     DTMGIAGRANLAGSVTTVNINELAVPAGTGSYTLATAQGGLAGARFKFGTLFGAMPIG
                     RTFDFRLSDTALRLTLRDSAGTFHWKGGVGGSWATPFANGVSNWTRTAGGRDYVYGTP
                     GGGVDVVFSGSSATAMGADFAVNSLRFAGGRHVLSDSNVLTLRAGGGRGVTVDSGSSA
                     VLGVNIVLAGNQSWLNDGALTISGSTIGGLGRNLTIDGFGTTIIAAGIQTGNGALRKR
                     GDGVLFLTGDSGLFTGSTTVEAGRLFVDGRLGGASLEVQGGALLGGNGLVPSLTIREG
                     GILAPGHSIGTMSVAGDVRFDPGAIYRVEVAAPTAGLLLGPSDCTVASGSLSAQGANV
                     EVAVDGTGYRPINQYLILATGGGIGGQFSGATSNAGYLRPSLDYAGNDVYLMLRRTDV
                     DFTSAGTRGNQTAVASALNGLVATATGGLATVINNLYDLPDDRALQAMTSMTGLLHHY
                     VARSAFDASRTFTKASAARLGMVASGDDRLVADSGLTAGLLKDPTPGSALPAYRGWVS
                     GLGADTAYAGVGVDAGASGDMRGLLFGFDASVARGVTLGVSGGRSWPRIGLEGLTDRA
                     TGSMTHVGAYGRYQRKRTRLDFVLGVGRFDNQTSREVTDGVTTLVARSEYGGSSVSGQ
                     IEYGYTVPLGRGFSVEPGIGFLFGRQHLDAFSEDGADVLGLSGPARREWSARVLIGSR
                     ASKSLGRAAGLRLMIEGRAAFSRDLEPLGNVFVRLRGDRSAGFRLSPPDFSRDAGVFG
                     LGLGAVTHRGIRLFADLDTETGGAITIFRGNVGLTKTW"
     gene            complement(3156..4424)
                     /locus_tag="PKK95_10440"
     CDS             complement(3156..4424)
                     /locus_tag="PKK95_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC18680.1"
                     /translation="MSMSARSRRDFLKAVAATGTASLVAPRGLDARTIRRSIGANDRI
                     RIGIIGCGSRGIGVEMASVRDHAKAENLEVVAVYDPWRVAAEEAAGKAKEWFGTTARQ
                     CRSVDDLLGLPEVDAVFISSPDHWHAAHLEAAARAGKHVYVEKPMANEIGELNRAYKA
                     AKESGVVIQAGTQLRSTPIAMGCRRLLESGTLGKLSRIEQVRNGDKPYWYEYLKPDVR
                     AADLDWKTFTKGRTSRPFDPIMYSGWYGYWEFSQGPVPQWGVHFLDLVHFIAKLGIPS
                     SCVCLGGVYTWKDEHRFTAPDQVQALWDYPEGLMISYSTNFGNSAGSLTRLCGDKGTL
                     NLNWSNAIYTADGGINRDGSIRGENKVEPIEQVDHWVNWYRCMRDKKTPNASLDAGYQ
                     HAVASIMATMSYETGRRARYDAAKRTITLE"
     gene            4417..4647
                     /locus_tag="PKK95_10445"
     CDS             4417..4647
                     /locus_tag="PKK95_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18681.1"
                     /translation="MLIVQLLAAVIGGAPGIKGGLPTGECNTSSRRSEGGRQGWAAQE
                     FEDPAVAARIRHIVEPANEQNLDRLVAALAGG"
     gene            complement(4695..6479)
                     /locus_tag="PKK95_10450"
     CDS             complement(4695..6479)
                     /locus_tag="PKK95_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18682.1"
                     /translation="MLAFALRLVRPYWLWVAAVLAAMLLETAMTLASPWPLKIVLDSV
                     LDSQPLPSVLGWVFASAPARLSVLKLAVAATVLIALLQAATAYANAYFTVSVGQWIAH
                     DLRLSIYAHLQRLSLSYYDRRQIGPLVSTITDDVNAVQDFVSTSLLDILIDSLTIAGM
                     LAVMLALNWRFTLIALAITPLVVIFAYRLRATFKQATRDLRRRQSELVSIVQEGLGAI
                     RVVKAFAQGRYERDRLGAKSLESVQAALHARRVRSLLGPFMTVVVALGTAGVLWFGAR
                     LVLTGAMTAGALVVFITYLGRLFRPIQNLARASSNVAQATVGLERIRAVLDADERLPR
                     SPGARPAGRLEGRVEFRRVTVGYNPGRLVLTDVSLVAQPGQLIGLVGPSGSGKSTLAS
                     LIPRFYDPLSGTVLIDGHDVREFTIRSLRSQIAIVLQDTQLFYATVLQNIAYGRPDAS
                     RDDIVEAARLAQAHDFIEALPDGYDTVVGQGGFTLSGGQRQRIGIARALVRDARILLL
                     DEPSSGLDAESERLVFEGLRNLLEGRTTFVIAHRLATVRHASCILVLDEGRIVERGTH
                     EELLASNGLYARLLAEQQPSDGASLRTP"
     gene            complement(6696..7046)
                     /locus_tag="PKK95_10455"
     CDS             complement(6696..7046)
                     /locus_tag="PKK95_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18683.1"
                     /translation="MESRARHFNVRAFAALVMTASGIGLPLTGYMHHVYGLEAMSVAR
                     HAWMSAHNLLGVLFTVSAVCHAWMNRRALLGHVRGARARLGAVNREAISAAALVLAGL
                     ALFVGHAFRAGGFR"
     gene            7313..7386
                     /locus_tag="PKK95_10460"
     tRNA            7313..7386
                     /locus_tag="PKK95_10460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7345..7347,aa:Gln,seq:ctg)
BASE COUNT         1111 a         2510 c         2686 g         1090 t
ORIGIN      
        1 gctcttcgcg ggcagcgtgg gtggcgacgg gtctggcggc gaggtgactg tgacccaggc
       61 cggcagcgtc acggtggccg gcgacggggc caacggcatc ttcgcacaga gtgccggggg
      121 acgatcgacg ggcgggcgcg tcagcgtcgc gctgaacagc ggcagcatcc tggcgaccgg
      181 cgccgggtcg aacggcatct tcgcacagag catcggcgga gggggcagcg gcgacgtatc
      241 ggtgaccatc gcgagcgcgg ccagcctggt ggtgggcggc tcgaacggag gctcggcggt
      301 ccgcttcgcc cacggaaacc gcaacacgct caccaactac ggcaccctca cgaccgtggg
      361 ggggatttcc ggccttgccg tcgttggcgg cacgggcaac gagaccgtgg acaaccacgg
      421 cacgatcacc ggttcggtga acctcggcgg cggggtgaac acgctgacca accgtgcaga
      481 agggctgctc aacgccgggt cgtcgatcga cgtgggcgcg ggcggcgagt tcgccaacgc
      541 cggcacgctc tcgcttggcg ggttgggccg cgcacagacg accgcggtga cgggcaactt
      601 cacgcagtcg ggcacgccga cgtggctggt ggacatccgc catccgggga cgtccgacac
      661 gatgggcatc gcgggccgcg cgaacctggc cggcagcgtg acgaccgtga acatcaacga
      721 gctggcggtg cccgcgggca cgggcagcta cacgctcgcc accgctcagg gcggcctcgc
      781 gggggcgcgc ttcaagttcg gcacgctttt cggcgccatg ccgatcggga ggacgttcga
      841 tttccgcctc tccgacaccg cgttgagatt gaccctgcgc gactcagccg ggacgtttca
      901 ctggaagggc ggagtgggcg gcagctgggc gaccccgttc gcgaacggcg tgtcgaactg
      961 gacccgcacg gccgggggac gcgactacgt ctacgggacg ccgggcggcg gcgtggacgt
     1021 ggtcttctcc ggatccagcg cgacggcgat gggcgccgac ttcgccgtga acagcctccg
     1081 cttcgccggc ggcaggcacg tgctctccga ctccaacgtg ctgaccctcc gggcgggcgg
     1141 tgggcgcggc gtcaccgtcg acagcgggtc gtctgctgtg ctcggcgtca acatcgtgct
     1201 cgctggaaac cagtcgtggt tgaacgacgg ggcgttgacg atcagcggat ccacgatcgg
     1261 cggccttgga aggaacctga cgatcgacgg gttcgggacg acgatcatcg ccgccgggat
     1321 ccagacgggt aacggggcgc tgcggaagcg gggtgacgga gtgctgttcc tgaccgggga
     1381 cagcggtttg ttcacgggca gcacgaccgt cgaggcgggc cggctgttcg ttgacgggcg
     1441 gctcggaggc gcctcgctcg aggtgcaggg cggcgcgctt ctcggcggga acggcctcgt
     1501 cccgtcgctc acgattcggg aaggcgggat cctggcgccg ggccactcga tcggcacgat
     1561 gagcgtcgcc ggcgacgttc gcttcgatcc cggcgccatc tacagggtgg aggtcgccgc
     1621 gccaaccgcc gggcttctgc tgggtccgag cgactgcacc gtggcgtccg gttcgctgtc
     1681 ggcgcagggc gcgaacgtcg aggtggccgt ggacggaaca ggctaccggc cgatcaacca
     1741 gtacctcatt ctcgcgaccg gcggcggcat cggcgggcag ttctccggcg cgacctcgaa
     1801 cgccgggtac ctgcgcccgt cgctcgacta tgccggcaac gacgtctacc tgatgcttcg
     1861 gcgcacagac gtggacttca cgtcggccgg cacgaggggg aaccagacgg ccgtggcgtc
     1921 tgccctcaac ggcctcgtgg cgacggcgac cggcggcctg gccaccgtga tcaacaacct
     1981 ctacgacctg cctgacgatc gcgccctcca ggccatgacg tccatgaccg ggctgctcca
     2041 ccactacgtc gccaggagcg ccttcgacgc ctcgcggacc ttcacgaagg cgagcgcggc
     2101 acgcctgggc atggttgcga gcggcgacga ccgcctcgtc gccgactcgg ggctcacggc
     2161 cggcctgctg aaggacccga cccctggttc cgccctgccc gcctaccgcg ggtgggtcag
     2221 cgggctcggg gccgacaccg cgtatgccgg cgtcggggtg gacgccggag ccagcggcga
     2281 catgcgcggc ctgctcttcg gcttcgacgc ctcggtcgcc cgcggcgtga cgctcggcgt
     2341 cagcggcggg cgcagctggc cgcgaatcgg tctcgaaggc ctcaccgacc gcgccaccgg
     2401 gagcatgacg cacgtcggcg cctacgggcg ctaccagcgg aagcgcaccc ggctcgactt
     2461 cgtgctcggc gtcggcaggt tcgacaacca gacctcgcgt gaggtcaccg atggcgtcac
     2521 gacgctggtc gcgcggtcgg agtacggggg aagcagcgtt tccgggcaga tcgagtacgg
     2581 ctacaccgtg cccctcggcc gcggcttcag cgtggaaccg ggcatcggct tcctgttcgg
     2641 ccggcagcac ctcgacgcgt tcagcgagga cggggccgac gtgctcgggc tgagcgggcc
     2701 cgcgcgccgc gagtggtccg cccgggtgct gatcggatcg cgcgcttcga agtcgctggg
     2761 gcgggcggcg gggctgcgcc tgatgatcga ggggcgggcg gcgttcagcc gcgacctcga
     2821 gccgctcggt aacgtgttcg tgcggcttcg cggcgaccgg tcggccggct tcaggctatc
     2881 acccccggac ttctcgcgcg acgccggcgt cttcggcctc ggcctcggcg cggtgactca
     2941 tcgaggcatc aggctgttcg ccgacctcga cacggagacg ggcggagcca tcacgatctt
     3001 ccgtggaaac gtcgggctga cgaagacctg gtagaggcgc gttgccgtgt accggcgact
     3061 gtccgggccg gtgtctcgga tgccgtacgg ggaggtggaa tggggcactc ctgccggccc
     3121 ccggggggcc gacaggagtg tcagagtgcg cggggctact cgagggtgat ggtccgcttc
     3181 gccgcgtcgt acctcgcgcg gcgtccggtc tcgtacgaca tcgtggccat gatgctcgcc
     3241 accgcgtgct ggtacccggc gtcgagcgag gcgttgggtg tcttcttgtc acgcatgcag
     3301 cggtaccagt tcacccagtg gtccacctgc tcgatcggct cgaccttgtt ctcgccgcgg
     3361 atcgagccgt cgcggttgat gccgccgtcc gcggtgtaaa tggcgttcga ccagttgagg
     3421 ttgagcgtcc ccttgtcccc gcaaagccgg gtgaggctgc cggcgctgtt gccgaagttg
     3481 gtcgagtagc tgatcatcag gccttcggga tagtcccaca gtgcctgcac ctggtcgggc
     3541 gcggtgaagc ggtgctcgtc cttccacgtg tagacgccgc ccaggcacac gcagctgctc
     3601 gggatgccca gcttggcgat gaagtgcacc aggtcgagga agtgcacgcc ccactggggg
     3661 accggaccct gcgagaactc ccagtacccg taccagcccg aatacatgat cgggtcgaag
     3721 ggcctgctcg tgcgcccctt ggtgaaggtc ttccagtcga ggtcggcagc gcggacgtcg
     3781 ggcttgaggt actcgtacca atacggcttg tccccgttgc gcacctgctc gatccgcgaa
     3841 agcttgccga gggtgcccga ctccagcaac cggcggcagc ccatcgcgat cggcgtgctg
     3901 cgcagctgcg tgccggcctg gatgacgacg cccgactcct tggcggcctt gtacgcccgg
     3961 ttcagctcgc caatctcgtt ggccatcggt ttctcgacgt agacgtgctt gccggccctg
     4021 gcggccgcct cgaggtgggc ggcgtgccag tggtcgggcg acgagatgaa caccgcgtcg
     4081 acctccggaa ggccgagcag gtcgtccacg ctccggcact gccgcgccgt ggtgccgaac
     4141 cactccttgg ccttgcccgc ggcctcctcc gccgcgaccc gccacggatc gtagacggcg
     4201 accacttcca ggttctcggc cttggcgtga tccctcacgc tggccatctc gacgccgatg
     4261 ccgcggctgc cgcagccgat gatgccgatg cggatgcgat cgttggcgcc gatgctgcgc
     4321 cgaatcgtcc gcgcatcgag gccgcgcggc gcgaccaggg atgccgttcc cgttgcagca
     4381 actgccttca gaaaatcacg ccgtgaccgt gcggacatgc tcatcgtgca actcctcgcc
     4441 gccgtaatcg ggggtgcccc ggggatcaaa gggggcttgc cgacggggga gtgtaacacc
     4501 tcgagccggc ggagcgaagg cggccggcag ggctgggcgg cgcaggagtt cgaggacccc
     4561 gcggtggcgg cccgcatccg ccacatcgtc gagccggcga acgagcagaa cctcgaccgg
     4621 ctcgtggcgg ctcttgctgg cggctgaggt cttgcggccc gcatccggcc ccttcgcggg
     4681 cgacgccggg ccagctatgg cgtccggagc gatgcgccgt cggatggctg ctgctcggcg
     4741 agcagccgcg cgtagagccc gttcgacgcg aggagctcct cgtgcgtgcc gcgctccacg
     4801 atgcgaccct cgtcgagcac gaggatgcag ctggcgtggc ggacggttgc gaggcggtga
     4861 gcgatgacga aggtcgtccg gccctcgagc aggttgcgca gcccctcgaa gacgagccgc
     4921 tccgactcgg cgtcgagccc cgagctgggc tcgtcgagca gcaggattcg cgcgtcgcgc
     4981 accagcgccc gcgcgatgcc aatccgctgg cgctggccgc ctgacagggt gaacccgccc
     5041 tgcccgacca cggtgtcgta gccatccggc agcgcctcga tgaagtcgtg tgcctgcgcg
     5101 agccgtgccg cctcgacgat gtcgtcgcgg ctggcatcgg gcctcccgta agcgatgttc
     5161 tgcaggaccg tcgcgtagaa gagctgcgtg tcctggagga cgatggcgat ctgagagcgc
     5221 agggagcgga tggtgaactc gcgaacgtcg tggccgtcga tgaggacggt gcccgagagc
     5281 gggtcgtaga agcgagggat caggcttgcc agcgtcgact tgccgctccc gctcgggccg
     5341 accagcccga tgagctggcc gggctgggcg accagcgaca cgtcggtgag aacgagccgc
     5401 ccggggttgt acccgacggt gacgcgacgg aactcgaccc gcccctccag gcggcccgcg
     5461 ggccgcgcac cgggcgaacg cgggagccgt tcgtcggcgt cgagcacggc ccggattcgc
     5521 tcgaggccga cggtggcctg ggcgacgttg gaagaggccc gcgcgaggtt ctggatcggc
     5581 ctgaacagcc tccccaggta ggtgatgaag acgacgagcg cgccggccgt catggcaccc
     5641 gtcagcacga ggcgagcccc gaaccagagc acgccggcgg tgccgagcgc gacgacgacg
     5701 gtcatgaagg ggccgagcag cgaccggacg cgccgcgcgt gaagggcggc ctgcacgctc
     5761 tcgaggctct tcgcgccgag ccgatcgcgc tcgtagcggc cttgcgcgaa cgccttcacc
     5821 acgcggatgg ccccgaggcc ctcctgcacg atgctcacga gctcgctctg gcggcgcctg
     5881 aggtcccggg tggcctgttt gaaggtggcc cggaggcggt acgcgaagat cacgacgagc
     5941 ggggtgatcg ccagcgcgat gagcgtgaag cgccagttca gcgccagcat caccgccagc
     6001 atcccggcga tggtgaggga gtcgatcagg atgtcgagca gcgaggtcga cacgaagtcc
     6061 tggaccgcgt tcacgtcgtc ggtgatcgtg ctgaccagcg ggccgatctg ccggcggtcg
     6121 tagtacgaga gggacagccg ctgcaggtgc gcgtagatgc tcagccgcag gtcgtgggcg
     6181 atccactggc cgacgctcac cgtgaaatag gcgttcgcgt aggcggtggc cgcctggagc
     6241 agggcgatca gcacggtggc cgcgacggcc agcttcagga ccgagagccg cgcgggggcg
     6301 gaggcgaaca cccacccgag caccgagggc agcggctgcg agtcgagcac cgagtcgagg
     6361 acgatcttga gcggccacgg cgaggcgagc gtcatggccg tttccagcag catggccgcc
     6421 agcacggctg cgacccagag ccagtacggc cgaaccaggc gcagggcaaa ggccagcacg
     6481 ggcgtcgatt gtactcggtc cgcaacggct gagatgccga tcacgtcctg cgccgtacgg
     6541 catgccccat gtacgagacg ctcaggtcgt cgctctcgtg gaagcccagc cgacggccca
     6601 gctcctcgaa gccgatcttc ccccgcacgc gacgtccgca gctcgcagca cgcgctcgaa
     6661 gtagccggcc gtgcggcggc cgaaacgaat ggcgctcatc tgaagccccc ggcgcggaac
     6721 gcgtggccga cgaagagcgc gagccccgcc aggacgagcg ccgcagcgga gatggcctcg
     6781 cggttcacgg cacccagccg cgcccgcgcg cctcgaacgt ggccgaggag ggcgcgacgg
     6841 ttcatccagg cgtggcagac ggccgacacg gtgaagagga cgccaagcag gttgtgcgca
     6901 gacatccacg cgtgccgcgc gaccgacatg gcctcgaggc cgtagacgtg gtgcatgtac
     6961 ccggtgagcg ggagcccgat gccgcttgcc gtcatgacga gcgcggcgaa cgcccgcacg
     7021 ttgaagtgcc tggcccggct ctccacgagt tctgcccttc tcctcagcga tacccctcta
     7081 cgaacgcccg cacctttccg agcaccgacg ctcaggcgcc caccggagtg acgtccgccc
     7141 cggcggggtt gcctccccga ggcggcgggg ctcctcagga accaggagcc gcgacggccg
     7201 ggcaaggaaa ggttccgcgc gcggccaccc cggcggccac cccggcggct tgcgcctggg
     7261 cggcccgtcc gctagaatga gagttcgttc gttcctgcct cgagactcct gctgggaggt
     7321 cgttcaacgg taggacacgt ggctctggac cacgttatcg gggttcgaat ccctgcctcc
     7381 cagccagcct tcgccaa
//
LOCUS       DAOPQX010000181         7341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11230_length_7341_cov_4.755147, whole genome shotgun sequence.
ACCESSION   DAOPQX010000181 DAOPQX010000000
VERSION     DAOPQX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11230_length_7341_cov_4.755147"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PKK95_10465"
     CDS             complement(<1..270)
                     /locus_tag="PKK95_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC18684.1"
                     /translation="MALDLFDLRGKVALVTGTSRGLGAGMAAALACAGADVAVHDRKA
                     PCSTEERLRALGVRTACLGGDLSDRGSADRIIAQTIERMGRLDILV"
     gene            complement(272..652)
                     /locus_tag="PKK95_10470"
     CDS             complement(272..652)
                     /locus_tag="PKK95_10470"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOC18685.1"
                     /translation="MSTTPTTAVSWLQRWPDVPAEEPADGIERRMVWGRRLMICRLRF
                     APHVVAAVHTHPHEQFTLVERGRVRFEVDGEQGIASAGAVLHFPSNVPHGATMLDEEV
                     VLVDIFSPVRDDFLEDGSGGRGEV"
     gene            complement(882..3134)
                     /locus_tag="PKK95_10475"
     CDS             complement(882..3134)
                     /locus_tag="PKK95_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOC18686.1"
                     /translation="MRPSRLLWALVALSLTAPASADDRQSVLVRAKKPYTSVVKSIQK
                     LGGTVTHQYKFVDGLAAEVPRSALPALEALVGAKNIGKDELVTIPESREQLTGYAVEA
                     QTVTPASAVPADYWTSHLYSGASQLFSAGHTGEGVIIAIIDSGFRPLFSHVSAARVLD
                     GASFVPGEPNAKDNANYPHGTQVAGIAAANISFCFADTARFAIEARTLGAGIPGSEGG
                     CPANTINIPMVGTAPLATIYPVKVFPRSGAGSPSSRTLAAMEHVLQQRLDYDAGRGGV
                     NIKVLNMSLGGPTSFAGRTLNDEMVTRLLDADIVTVVSMGNNGSAGITGGSPGTAMAA
                     LTVGAASLPQSEWIYRAQFSSPCSTAPADPAILHACATLWRPDTHVQVADYSSRGPTH
                     DGRMKPEIVASGSYSFTQGSGSSTTVNFVLGTSFSSPEVAGVAAALRGAVSGATARQV
                     RNALILTANPSLAPAAEAFDQGHGYLDAVAALAMLRAGGASDTVNLDSKYTRNLQANL
                     DRAGMPVYSGSVSAVETGVLPAERREFAFQVPKNAAKLYVTFRDIVAGASQNTFFGDD
                     LLVMVQGNTVHDDDYRELEEDVTYAVIPAGAQKTFEFDAPSEGIWRITPQGDWTNASP
                     ISFGIDIWTTEAALPRHTAKANIGQGQQHAYTLKVPAGTQSLAVRASWLNMNANVPIN
                     DLDVYLLPPGGSLVVDCATARTPEACTISEPAAGDWRVIVEGFAVYPESPGGRETYTL
                     RVAADDVVLK"
     gene            3262..3771
                     /locus_tag="PKK95_10480"
     CDS             3262..3771
                     /locus_tag="PKK95_10480"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOC18687.1"
                     /translation="MFGLISLSAILVLVPAAAAGPRAADRAVPASLRAASPTVLRQQN
                     TSTVDLVQVQLVLPKKVRVGKTFSVLDEVENQGESFAANSVTGFYLSLDDQFDEKDLL
                     VGARRVPQLASQQAHNTVTPVTIKAPVPAGDYYLIALADARHDLEERYRSNNTRAAKV
                     TVEPAKEKE"
     gene            3783..4568
                     /locus_tag="PKK95_10485"
     CDS             3783..4568
                     /locus_tag="PKK95_10485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC18688.1"
                     /translation="MNGYEISAGLYDHVGLYRERLDDVRYYVAQAAAAGGPVLELGCG
                     TGRVMLPCARAGARITGLDRSAPMLAVARAKLEREDAGVRGRAQLVEGDMRSFDLGER
                     FALVTIPFRGFQHVLAVEDQIACLENVHRHLEPGGLVALDVFNPMLQALVDRSRLAEW
                     GDEPEFTTPERARVVRRFRVERVDLPRQVQYLEMIYYVTEPDGRSRRLVEQFSMRHFF
                     RYEVEHLLARCGFTDIRIDGDFAGTPFDAAGDPAEIVALARRR"
     gene            4709..5188
                     /locus_tag="PKK95_10490"
     CDS             4709..5188
                     /locus_tag="PKK95_10490"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC18689.1"
                     /translation="MDHKALFMKYWEREAPATRKVLSRIPEGSDYRPDPKSRTAREIA
                     WLIVQEERALAEGLEKARLEWADVPAPATMKEVLEAYDRQHDDLTRRMNAVDAAVWEG
                     KIPFMFGGQEVMSAPGYDNAWGFLLDMIHHRGQLSTYLRPMGSTVPQIYGPSADEPM"
     gene            5307..6044
                     /locus_tag="PKK95_10495"
     CDS             5307..6044
                     /locus_tag="PKK95_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOC18690.1"
                     /translation="MRATHLAAAVLFGAALVSTIPLHAAAQEPHQPPPSIQVTGEATV
                     TVQPDQVQIEIGVVTQAEKSEAAASRNAQQLDKVLADLRGVVGPEGKVQTIGYSLRPD
                     YRYPGQGGGEPTISGYTATNVVRVTLNDLTRVARAIDAATGSGANRIQALRFELKDES
                     PVLAQALREAASRARAKADALAAALNLQIVRVLSVSESGGGPVPVVPVRDVMFARAEA
                     AAPPTPIEPGTVEVRATVNLVVEVRGS"
     gene            complement(6140..6865)
                     /locus_tag="PKK95_10500"
     CDS             complement(6140..6865)
                     /locus_tag="PKK95_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18691.1"
                     /translation="MVKYGCALLVILTVGFACALPVSAQPDGFRVEVAGGLGLIGIRP
                     VVRACCELRTPPAFTEVGSHEGAVEPAFGVRVRESGRTSTAVQAGWARTGEVVLSYPP
                     VEPQPPLSAYTSERRLRARTFTVSASQALDFPLGDRTRGYLAAGLVLSRQSWNQRDTS
                     IRVDDPAVHRVEEAGWSFTRPGGALAGGLKLYTGKRFFAAAETGAVFLAAKEGEDENR
                     ARWAVGRETRFFLRLQIGAGFLR"
     gene            complement(6950..>7341)
                     /locus_tag="PKK95_10505"
     CDS             complement(6950..>7341)
                     /locus_tag="PKK95_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18692.1"
                     /translation="SDLSAKRGIADEAEATGEAIKAGASSSSEIVRTLAARFTDGQRP
                     TLPRIPNQPSEAQPALIDGCKAAWELVKAKAPGEADAYARFLLDAAKGASSGAREGGF
                     IGIGIAKVSEKEQAALAALALALGLPF"
BASE COUNT         1146 a         2521 c         2567 g         1107 t
ORIGIN      
        1 caccaggatg tcgagccggc ccatccgctc gatggtctgg gcgatgatcc ggtcggccga
       61 accccggtcg gacaagtcgc cgccgaggca cgcggtgcgg acgcccagcg cccgcaggcg
      121 ttcctcggtg gaacagggag ccttgcggtc gtgcaccgcg acgtcggcgc cggcgcaggc
      181 gagcgccgcg gccatgcccg cacccaaccc ccgcgacgtg cccgtgacca gcgctacttt
      241 gccccgaaga tcgaaaagat cgagagccat ctcacacctc tccccgcccg ccggagccat
      301 cctcgagaaa gtcgtcgcgg accggcgaga agatgtccac cagcaccacc tcttcgtcga
      361 gcatggtggc gccgtgcggg acgttcgacg ggaagtggag cacggcgcct gccgaggcga
      421 tcccctgttc cccgtccacc tcgaagcgca cccggccgcg ctcgaccagg gtgaactgct
      481 cgtgcgggtg cgtgtggacg gcggccacca cgtgcggcgc gaagcgcagg cggcagatca
      541 tcagccgccg gccccacacc atcctgcgct cgatgccgtc ggccggctct tccgccggga
      601 cgtccggcca acgctgcagc caggacacgg cagtcgtggg agtggtgctc atcggcgttc
      661 cgctttgccc gtgtcgagcc ccaacccgag cgcggagtac tgatccggaa ccgcgcgggg
      721 tcgatgcgtc gcatgggcga gaaagcctcc gtggcggagg gcagccggag ctactttgtc
      781 agaaccgcaa acaaattacc aggaagcggg ccgggcggcg cgggcctcgc aacccgcgcc
      841 gccggctgag gcgggcttac ttgagcacga ttgaggcggg cttacttgag cacgacgtcg
      901 tcggccgcga cgcgcagcgt gtacgtctcg cggcctccgg ggctctcggg gtagaccgca
      961 aagccttcga cgatcactct ccagtcgcct gcggcgggct cgctgatcgt gcacgcctcg
     1021 ggcgtcctcg ccgtggcgca gtcgaccacc agggagccgc ccgggggaag caggtacacg
     1081 tccaggtcgt tgatcgggac gttggcgttc atgttgagcc agctcgccct caccgccagc
     1141 gactgcgtcc ctgccggcac cttcagcgtg taggcgtgct gctggccctg gccgatgttc
     1201 gcctttgccg tgtgccgcgg aagcgcggcc tcggtcgtcc agatatcgat gccgaacgag
     1261 atcgggctcg cgttggtcca gtcgccttgg ggagtgatcc gccagatgcc ttccgacggg
     1321 gcgtcgaact cgaacgtctt ctgcgccccg gccgggatga cggcgtacgt gacatcctcc
     1381 tccagttcgc ggtagtcgtc atcgtggacc gtgttcccct ggaccatcac gagcagatcg
     1441 tctccgaaga acgtgttctg gcttgcgccg gcgacgatgt cacggaacgt gacgtacagc
     1501 ttggcggcgt tcttcgggac ctggaacgcg aactcgcgcc gctcggcggg cagcacgccg
     1561 gtctcgacgg cggacacgct ccccgagtag acgggcattc ccgcgcggtc caggttcgcc
     1621 tggaggttgc gcgtgtactt ggaatcgaga ttcacggtat ccgaggcacc gccggcccgg
     1681 agcatggcca gggccgccac ggcgtcgagg tagccgtgcc cttgatcgaa ggcctccgcg
     1741 gccggggcca gcgaagggtt ggccgtgagg atcagggcgt tacgcacctg gcgcgccgtc
     1801 gcgccggaaa ccgctccccg cagggccgcc gccactcccg ccacctcggg cgacgagaac
     1861 gaggtgccca agacgaaatt caccgtcgtg gacgaccccg aaccctgcgt gaaggagtag
     1921 ctgccgctgg cgacgatctc cggcttcatc cggccatcgt gcgtggggcc cctcgagctg
     1981 tagtcggcaa cctggacgtg agtgtcggga cgccacaaag tggcgcaggc gtgcagaatc
     2041 gccgggtcgg caggcgccgt gctgcaggga gacgagaact gcgcacggta aatccactcg
     2101 ctttgcggca gcgatgcggc cccgaccgtc agcgccgcca tcgccgtgcc cggcgacccg
     2161 ccggtgatac ccgcgctgcc gttgttgccc atcgagacga cggtcacgat gtcggcgtcc
     2221 aggagcctgg tgaccatctc gtcgttgagc gtccggccag cgaacgacgt cggcccgccc
     2281 aggctcatgt tgagcacctt gatgttcacg ccgccccggc ccgcgtcgta gtcgaggcgc
     2341 tgctgcagca cgtgctccat cgcggccagc gtgcgcgacg agggcgatcc ggcgccggag
     2401 cgggggaaga ccttgaccgg gtagatcgtc gccaacggcg cggtgcccac catcggaatg
     2461 ttgatcgtat tggcggggca cccgccctcc gagcccggga ttcccgcccc cagggtgcgt
     2521 gcctcgatgg cgaacctcgc cgtgtcggcg aagcagaaag agatattggc tgccgcaatg
     2581 cccgccacct gcgtgccgtg cgggtagttc gcgttgtcct tcgcgttcgg ctcgccgggc
     2641 acgaagctcg cgccgtccag cacccgggcc gcgctgacgt gcgagaagag ggggcggaag
     2701 ccgctgtcga tgatcgcgat gatgacgccc tcgccggtat gtcccgcgct gaacaactgc
     2761 gaggccccgc tatagaggtg ggacgtccag tagtcggcgg gaacggcgga ggccggcgtg
     2821 acggtttgag cttcgacggc gtagccggtc agttgctcgc gcgactcggg aatggtcacc
     2881 agttcgtctt tgccgatgtt cttcgcgccc acgagcgcct cgagggccgg aagggccgac
     2941 cgcggcactt ccgcggccag gccgtcgacg aacttgtact ggtgggtgac ggtgccaccg
     3001 agcttctgaa tgctcttgac cacgctggtg tagggcttct tggcccggac gagaacgctc
     3061 tgcctgtcgt cggccgaagc cggggccgtc agcgacaggg cgaccagggc ccagagcaga
     3121 cgcgagggac gcatacctgc ctcctgatag gggtgtatgg ctgagattgt ttcatcatat
     3181 cctcgtgcga ttcggatagg aagggcgttt gggctatcat tcgcctgccc gctcgtaaac
     3241 agccgcaggg agggtcacga catgttcggc ctcatctccc tctcggcaat cctggtgctc
     3301 gttccggcgg cggccgccgg gccccgcgct gcggatcgcg ccgtgcccgc ttctcttcga
     3361 gccgcatccc ccaccgtctt gcggcagcag aacacgagca ccgtggatct cgtccaggtg
     3421 cagctcgtgc tcccgaagaa ggtgagggtc ggcaagacgt tctcggtgct cgacgaggtc
     3481 gagaaccagg gcgagtcgtt cgccgcgaac tcggtgaccg ggttctacct gtcgctcgac
     3541 gaccagttcg acgagaagga cctgctggtc ggcgcgcggc gcgtcccgca gctcgcgagc
     3601 cagcaggcgc acaacaccgt gacgcccgtg accatcaagg cgcccgtccc ggccggcgac
     3661 tactacctca tcgcgctcgc cgacgcccgg cacgacctcg aggagcgcta tcgcagcaac
     3721 aacacccggg cagccaaggt caccgtggag ccggcgaagg agaaggagta aggcgacttc
     3781 agatgaacgg ctacgagatc tcggccggcc tgtacgacca cgtcggactg taccgggagc
     3841 ggctggacga cgtgcggtat tacgtcgcgc aggcggcagc ggcgggcggg ccggtgctcg
     3901 agctggggtg cggcaccggc cgcgtgatgc tgccctgcgc gcgcgcgggc gcgcggatca
     3961 ccggcctcga ccgctcggcc ccgatgctgg ccgtcgcccg cgcgaagctg gagcgcgagg
     4021 acgccggcgt ccgcgggcgg gcgcaactcg tcgagggcga catgcgctcg ttcgaccttg
     4081 gcgagcgctt cgcgctggtg acgatcccgt tccgcgggtt ccagcacgtg ctggcggtcg
     4141 aggatcagat cgcttgcctc gagaacgtcc accgccacct ggagccgggc ggcctcgtcg
     4201 ccctcgacgt cttcaatccc atgctccagg cgctggtgga tcgctcgcgg ctcgccgagt
     4261 ggggcgacga gccggagttc accacccccg agcgcgcccg cgtcgtccgt cgcttccggg
     4321 tggagcgcgt ggacctgccg cgccaggtgc agtacctcga gatgatctac tacgtgacgg
     4381 agccggacgg ccgctcgcgc cggctcgtcg agcagttctc catgcgccac ttcttccgct
     4441 acgaggtgga gcacctgctc gcccgttgcg gtttcaccga catccgcatc gacggggatt
     4501 tcgcgggaac gcccttcgac gccgccggcg acccggccga gatcgtggcg ctggcgcggc
     4561 ggcggtaggt gatgtcgctg ccagtggaag gcgggcggcc cttgaccgtc gttatactgg
     4621 cggtagccgc cgcgggctcg gcggacccgc cgcgaattcg tggggagtgc gtgctgccct
     4681 gcccgtcagc gagaaggagg agggaaccat ggaccacaag gcgctgttca tgaagtactg
     4741 ggagagggaa gctccggcca ccaggaaggt gctgtcgcgc atccctgaag gatccgacta
     4801 ccgccccgac ccgaagtcgc ggacggcgcg cgaaatcgcc tggctgatcg tgcaggagga
     4861 gcgtgccctg gccgaagggc tggagaaggc caggctcgag tgggccgacg tccccgcacc
     4921 ggcgacgatg aaggaggtgc tcgaggcgta cgaccgccag cacgacgacc tgacgcgccg
     4981 gatgaacgcg gtggatgccg cggtgtggga ggggaagata ccgttcatgt tcggcggcca
     5041 ggaggtgatg agcgcaccgg gctacgacaa cgcgtggggc ttcctgctcg acatgatcca
     5101 ccaccgcggc cagctgtcca cctaccttcg ccccatggga tcgacggtgc cgcagatcta
     5161 cggacccagc gccgacgaac cgatgtgacg cggaacgagg gtcgagcgag ccgccccggg
     5221 cctgcaagaa accccgggcc gaacgttcag accatgaagg ctgactcctg ccgaacgcgg
     5281 gagagcccgg tctccgggag gacacgatga gagccacgca cctggccgcc gccgtgctgt
     5341 tcggcgcggc cctcgtttcg acgatcccgc tacacgctgc cgcgcaagag ccgcaccagc
     5401 ccccgccctc gatccaggtc accggcgagg cgacagtcac ggtgcagccc gaccaggtcc
     5461 agatcgagat tggcgtggtg acgcaggccg agaagtcgga ggccgcagcc tcgcggaacg
     5521 ctcagcagct cgacaaggtc ctggccgacc ttcgcggcgt cgtcgggccg gagggcaagg
     5581 tgcagacgat tggatactcg ctgcggccgg attaccggta tccggggcag ggcggcggcg
     5641 agcccaccat ctcgggctac accgccacca acgtggtccg cgtgaccctg aacgacctga
     5701 cccgcgtggc ccgcgcgatc gacgcggcga ccggctcggg ggccaaccgg atccaggccc
     5761 tccggttcga gctgaaggac gagtcgccgg tcctcgcgca ggcgctgcgc gaggcggcat
     5821 cgcgcgcgcg tgcgaaggcc gacgccctgg cggccgcgct caacctgcag atcgtacggg
     5881 tgctctcggt gtcggagtcg ggcggcggtc ccgtgccggt ggttccggtg agggacgtga
     5941 tgttcgcgcg tgccgaggcc gccgcgccgc cgacgccaat cgagccgggc accgtggagg
     6001 tgcgcgccac ggtcaacctt gtcgtcgagg tgcgaggctc ctgatcgact cccccgatcg
     6061 cggcggtacg atcgcggctc gcggcgaagg ccgcgggcgt gagcttagcg gcccctgagc
     6121 ggttgtggtg cgcaaaggtt caacgcagga acccggctcc gatctgaaga cgcaggaaga
     6181 agcgggtttc gcggccgacc gcccaccgtg cgcggttctc gtcttcgcct tccttcgccg
     6241 cgagaaagac ggcaccagtc tcggcagcgg cgaagaaccg tttgcccgtg tagagcttca
     6301 atccgccggc gagcgccccg cctggcctcg tgaacgacca cccggcctcc tccacgcggt
     6361 gcacggcagg atcgtccacg cggatcgacg tgtcgcgttg attccaggat tgacgagaga
     6421 ggacgagacc ggcggccagg tatccacgcg tgcgatctcc aagcgggaag tcgagtgctt
     6481 gcgaggccga caccgtgaag gtccgcgccc gaagccggcg ctcggacgtg tacgcggaca
     6541 gcggcggctg cggctcgacc ggggggtagc tcagcacgac ttcaccggtg cgtgcccagc
     6601 cggcctgcac ggcagtgcta gtgcgccccg actcgcggac ccgaacgccg aatgccggct
     6661 cgacggcgcc ctcgtgcgac ccgacctccg tgaaggcggg aggggttcga agctcgcagc
     6721 aggctcgaac gacgggccga atgccgatca gcccgaggcc ccccgccacc tcgacccgaa
     6781 agccgtccgg ttgggcggag acaggcaggg cgcaagcgaa cccgacggtg aggatcacca
     6841 gcagcgcaca cccgtacttg accacgtcga cctccggatg ttccgagcgg attctcggcc
     6901 ctcgcgccga gcgggtcaag cgactcttcg agtgtcgaaa cgagcgggtt cagaagggca
     6961 gccccaaggc gagggccagg gcggcgagcg ccgcctgctc cttctcactc accttggcga
     7021 tgccgatgcc gatgaagccg ccctcccgcg cgccgctgct cgcccccttt gccgcgtcca
     7081 gcaggaacct ggcgtatgcg tcggcctccc cgggtgcctt ggccttgacc agctcccacg
     7141 cggccttgca gccgtcgatg agcgccggct gggcctcgga gggttggttg gggatccgcg
     7201 gcagcgtcgg ccgctgtccg tcggtgaagc gcgccgcgag cgtccgcacg atctcgctcg
     7261 acgaactcgc gcccgccttg atcgcctcac cggtggcctc ggcctcgtcg gcgatgccac
     7321 gcttcgcact caggtcgctg t
//
LOCUS       DAOPQX010000182         7197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11523_length_7197_cov_4.288155, whole genome shotgun sequence.
ACCESSION   DAOPQX010000182 DAOPQX010000000
VERSION     DAOPQX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11523_length_7197_cov_4.288155"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1159)
                     /gene="menC"
                     /locus_tag="PKK95_10510"
     CDS             complement(53..1159)
                     /gene="menC"
                     /locus_tag="PKK95_10510"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HOC18693.1"
                     /translation="MRIERIELRHVRLVLVAPFVTSMGVEVDEEHIIVRVDAEGVTGW
                     GESVAEGTPFYSYETTQTAWHILRDFLVPGILGRDLASVDQAIALNAKVRGHMMAKAG
                     LESALWDAFAKAAGLSLSKMLGGVRERIEVGVSIGIQPTPAELLKKIAGYLEEGYRRI
                     KIKIAPGCDLEFVKAVRLEHPDIPFQVDANSAYELDDVGVFRAMDEERLLLIEQPLSH
                     DDIYEHSKLQRELKTPICLDESIHSLADARAALELGSCRVINIKPGRVGGFTESKRIH
                     DLCASRGVPVWHGGMLESGIGRAGNVALASLPNFTLPGDISASRRYYREDIVTPEFEV
                     ARDGTMAVPTGPGIGVEVVADRLDKVTLRREVFQ"
     gene            complement(1230..3449)
                     /locus_tag="PKK95_10515"
     CDS             complement(1230..3449)
                     /locus_tag="PKK95_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC18694.1"
                     /translation="MLPRPLPAAGPACLVAALAAAALVISLGSASAQETCTLVVRVQD
                     TTGVLPGASVVIARRADRATWRAAADGEGRARFERLPPGVYDIKSSLQGFADAERRGV
                     EVAPGEERAAEVTMTVVHFATSVTVTTASRREELLLEVADPTTLIDRTQIEDSGARSA
                     KDLLVEQSGSGVQVQAGGGQGHVSLNGIPNSGVLVLVDGRRFLGKDANGNLNLEELQL
                     AGIDRVEIVKGAASALYGSDALGGVINLITRKVLQPGVRNTMSVTGGSYGDLRVSDTI
                     GVRHGPGGFTATGQYRSYDGFDLDASNPQTIGQPESRFSNAGGTADLRLADRLDLRFF
                     GDYSRRKISNYFFSGPTQLPSTVYDSRRDLTRYTVSPELSFLPRPGTTLTASFTYGRY
                     DRDETQVFPDRQVVAVPWREWNREFKATGRHDWTLFGRTHALQAGYEFRDETLDRASL
                     RTDSGAARASRDINIGWLQQEFHAAARLKLTGGFRFDSYSDFGSSWSPKLSAVYAASA
                     RHSLRATYGHGFRAPFFGELFLFTPTFVGNPDLEPERSRTLSVGYTFGGTTAQLSADY
                     FRAQIEDGITFDLRQLPFTYQNLREYTSQGVNLAVSVQLPGGFVPSASYAYVHREDQS
                     GAEVGGLPTHTAFVKLLWANPRLGLRANLRAQILNEARFEDGTSQPPYQVWYAHVSKR
                     LLTGRPAALSVFVQADNLFDRRNIFLRSATGEPIPGDYQVWLSPRTFLGGVTIELGR"
     gene            3917..6853
                     /locus_tag="PKK95_10520"
     CDS             3917..6853
                     /locus_tag="PKK95_10520"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOC18695.1"
                     /translation="MTRAPFALALLLAALALTPAGAAGQESTTGTISGQVVDPQGLPL
                     PGATVVVISPQGSRTYVTDSQGRFHAPFLTPGRHNVRVELASFRPVTIENIEVQLGQR
                     VTMPPITMNLAQVAEQVEVRATPPAVDTTSSGMGANLNSDFLARLPVQRQMASVVYLS
                     PGVSTSGGAGEANPSISGASGLENQYVVDGINVSNTGYGGVGSYSIVFGSLGTGVPFD
                     FIDEVQVKVGGYEAEFGQATGGVVQAVTKSGANTFSGGVFGYWQPEEFEADYRETVLP
                     NATRANEAVNTRETFFSDVGATIGGPIVRDRVFFFGAVDRSWERTGLIAPEGFPLRDQ
                     GPVDRKRNITSYSAKGTFQVADAHRFDVSAFGDPSEGKSGPQRRSALLGENLTSRFSE
                     LDFGGHSQTVRYEGIITPSFLVTGSFSRATNAIEELPSLNEYSIIDTTTTPNLRFGGI
                     GFFENNEGENLQYQAKATYLVGDHELRGGVLYEDISYDNIVGRTGPTFVLPNGEPTAT
                     GASVSVLPDPNFGRIYRVTRANLTNVRATEQRYTSFFLQDTWKVHPTLTIKPGIRYEQ
                     QKLVGNLADHQWDGNWAPRIGAVWDPTGQGRLKIFGNWGRYYAKVPNDLAARALSADA
                     GATRADYFDESLTQPVPDGVEAAGTTQHLLLAGLSPADFDPDSKSTYSDEWLVGGEVE
                     VIPQLSVGVNYQHRNFGRILEDIGQAPMVAYFLEPGDQLASVEYFITNPDANTPVAFQ
                     EKYGASFEEAIHDYDAVTFTLEKRFANRWGLQSSYRLAWLEGTFEGFFRNDNGQSDPA
                     ITSLFDFPTNDPSYTLLGGPLGFRGDIRFLGAAGQGPLPLDRRHSIKVYGNYLFDFGL
                     NFGVGMTALSGTPLTALASNPVYDNNGEIPETPRGAGFETVDGFLDRTPWDTRLDLHA
                     DYSIPLGRQRLTLIAQVFNVFNRREPLDYDNYTEVSFGVPNPDFGRVIAYQAPRSLRV
                     GARFVF"
     gene            complement(6917..>7197)
                     /locus_tag="PKK95_10525"
     CDS             complement(6917..>7197)
                     /locus_tag="PKK95_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18696.1"
                     /translation="ELERAVELGSLEAPRPHHARAWVALGDAHYWRLSDLGKAREIWT
                     EGARRFPADAALRERLSKEGMPLRDAIRKTMNPDARVDTSLAELRAAQ"
BASE COUNT         1137 a         2501 c         2477 g         1082 t
ORIGIN      
        1 tcacacaggc gggacgcctg tgccactcgg tcacacaggc gggacgcctg tgctactgga
       61 atacttcgcg gcgcagggtc accttgtcga gacggtcggc caccacctcg acgccgatcc
      121 cgggaccggt gggtaccgcc atcgtcccgt cccgcgccac ctcgaactcg ggcgtgacga
      181 tatcctcccg gtagtagcgg cggctggcgg agatgtcgcc gggaagcgtg aagttgggga
      241 gcgaggccag cgcgacgttc cccgcccggc cgatgcccga ttccagcatg ccgccgtgcc
      301 agaccggcac cccgcgcgag gcgcagaggt cgtggatccg cttcgactcg gtgaagccgc
      361 cgacacgccc cggcttgatg ttgatcacgc ggcagctccc cagctcgagc gcggcgcggg
      421 cgtcggccag cgagtggatg ctctcgtcga ggcagatcgg agtcttcagc tcgcgctgca
      481 gcttcgagtg ctcgtagatg tcgtcgtgcg agagcggctg ctcgatcagt agcaggcgct
      541 cctcgtccat ggcgcggaag actcccacgt cgtccagctc gtacgccgag ttggcgtcca
      601 cctggaaggg gatgtcggga tgctcgaggc gcaccgcttt cacgaattcg aggtcgcatc
      661 ccggcgcaat cttgatcttg atccgccggt agccctcttc gaggtagccg gcgatcttct
      721 tcagcagctc cgcgggcgtc ggctggatgc cgatgctgac gccgacctcg atccgctcac
      781 gcacgccgcc cagcatcttc gagagcgaca ggccggccgc cttggcgaag gcgtcccaca
      841 gggccgactc gaggccggcc ttcgccatca tgtggccgcg aaccttcgcg ttgagggcga
      901 tcgcctgatc taccgaggcc aggtcgcggc cgaggatgcc cgggacgagg aagtcgcgaa
      961 ggatgtgcca ggccgtctgg gtggtctcgt acgagtagaa cggcgtcccc tcggcgaccg
     1021 attcgcccca gcccgtcact ccctcggcgt ccacccggac gatgatgtgc tcctcgtcca
     1081 cctcgacgcc catcgaggtg acgaacggcg ccaccagcac gagcctgacg tgcctcaact
     1141 cgatccgctc gatgcgcatg caaatacccg cggcccacgg ccgagggcat tctattacgt
     1201 cccgaatcgc gattcccgaa tcctgatccc taccgcccca gttcgatggt cacgccgccc
     1261 aggaaggtcc gcggcgagag ccacacctgg taatcgcccg ggatcggctc gcccgtggcc
     1321 gaccggagga agatgttccg gcggtcgaac aggttgtcgg cctgcacgaa caccgacaac
     1381 gccgcggggc ggcccgtgag gagccgcttg ctcacgtgcg cataccagac ctggtaaggt
     1441 ggctgcgacg tgccgtcctc gaagcgcgcc tcgttgagga tctgcgcccg caggttcgcc
     1501 ctcaacccga ggcgcgggtt ggcccagagc agcttcacga acgccgtgtg cgtcggcaac
     1561 ccgccgacct ctgccccgga ctgatcctcg cggtgaacgt aggcgtagga ggccgacggc
     1621 acgaagcccc ccggcagctg caccgacacc gcgaggttca ccccctgcga ggtgtactcg
     1681 cggaggttct ggtacgtgaa cggcagctgc cgcaggtcga aggtgatgcc gtcctcgatc
     1741 tgcgcccgga agtagtcggc cgagagctgc gccgtggtcc cgccgaacgt gtagcccacg
     1801 ctcagcgtgc gagaccgctc gggctcgaga tcggggtttc cgacgaaggt cggtgtgaag
     1861 aggaacagct cgccgaagaa cggcgcccgg aacccgtgac cgtaggtagc gcggaggctg
     1921 tggcgggccg acgcggcgta gacggcgctc agcttggggc tccagctgct gccgaagtcg
     1981 ctgtagctgt cgaaccggaa cccgccggtc agcttcagac gggcggcggc gtggaactcc
     2041 tgctgcagcc acccgatgtt gatgtcgcgc gacgcgcggg cggccccgct gtcggtgcga
     2101 aggctggcgc ggtcgagcgt ctcgtcacgg aactcgtagc ccgcctgcag ggcgtgggtc
     2161 cggccgaaga gcgtccagtc gtggcggccg gtcgccttga actcccggtt ccactcccgc
     2221 cagggcaccg cgaccacctg gcggtccggg aacacctgcg tctcgtcgcg gtcgtaccgg
     2281 ccgtaggtga acgacgcggt gagcgtggtg ccgggccgcg gcaggaacga cagctcgggc
     2341 gacacggtat agcgggtgag gtcgcggcgc gagtcgtaga cggtcgacgg cagctgcgtc
     2401 gggccggaga agaagtagtt ggagatcttc cggcgggagt agtcgccgaa gaaccgcagg
     2461 tcgagccggt ccgccaggcg cagatcggcc gtccctcccg cgttcgagaa gcggctctcg
     2521 ggctgcccga tcgtctgcgg gttggaggcg tcgaggtcga agccgtcgta cgagcggtac
     2581 tgccccgtgg ccgtgaagcc gcccgggccg tgccggaccc cgatcgtgtc ggacactcgc
     2641 aggtcgccgt acgaaccgcc ggtgaccgac atcgtgttac ggacgcctgg ctggagcacc
     2701 ttccgcgtga tcaggttgat gaccccgccg agcgcgtccg atccgtacag cgccgacgcc
     2761 gcgcccttca cgatctccac gcgatcgatc ccggccagct gcagttcctc gaggttgagg
     2821 ttcccgttgg cgtccttccc gaggaagcgg cggccgtcca ccagcaccag cacgccgctg
     2881 ttggggatcc cgttcagcga gacgtggccc tggccgccgc ccgcctgcac ctgcaccccg
     2941 ctgcccgact gctccaccag caggtccttg gcgctgcggg cgcccgagtc ctcgatctgc
     3001 gtcctgtcga tcagcgtggt ggggtcggcc acctcgagca gcagctcctc ccgccggctt
     3061 gccgtcgtca cggtgaccga ggtggcgaag tgcaccacgg tcatggtcac ctcggcggcg
     3121 cgctcctccc ccggcgccac ttccacgccg cgccgctcgg cgtcggcgaa accctggagg
     3181 ctggacttga tgtcgtagac gccggggggg aggcgctcga agcgcgcccg tccctcgccg
     3241 tcggccgcgg cacgccaggt ggcgcggtca gcgcgcctcg cgatcacgac cgaggcaccg
     3301 gggagcacgc ccgtggtgtc ctgcacgcgg acgacgaggg tgcaggtttc ctgggccgag
     3361 gcgctgccga gggagatgac gagcgccgcc gccgcaaggg cggcgacgag acaagccgga
     3421 ccggcggccg gcagggggcg cgggagcatt ggcacctctt cggatgttcg agatcgggtg
     3481 gattccctag tcagcagact agcagagggg ctgcacgggg acaacaacgg ggccaccagt
     3541 cgctcgtggc gtggatgtgc cggccggtgc ccgcaccgcc gcgtctgcct cgcgaactgc
     3601 cgagtcccgg ccctcgaggt cgcgcctctg atccggtaat ccagatcggg cgatccaagg
     3661 ccgcgaatcg gccgggtcgc gccggcccat ccgctggcgg cgcaaacaaa gcgggtgggg
     3721 ccctaagccc gaccaagatg gtcccatatt ttcaagcccg ctcgggggcg agcgacagct
     3781 gtcgcgtgac caacgtgccc ggggccggga cgaataccga cgaggtgcgg attcttgcac
     3841 aaggaacgtg tgcggaccgc gcccctccac gaccagccgc tggagcgagg gcgttcggtt
     3901 tccgcaggag gaaaagatga ctagagcccc ctttgcgctg gcgctgctcc tggccgcgct
     3961 ggcgctcacc cccgccggtg ccgccgggca ggagtccacc acggggacga tttccggcca
     4021 ggttgtcgac ccgcaggggc tgcctcttcc gggcgcgacc gtggtcgtga tctccccgca
     4081 ggggtcgcgg acctacgtta ccgactcgca gggccgcttt cacgcaccgt tcctgacgcc
     4141 cgggcgccac aacgtgcgcg tggagttggc gagcttcagg ccggtgacca tcgagaacat
     4201 cgaggtgcag ctgggtcagc gggtcaccat gccgccaatc acgatgaacc tggcgcaggt
     4261 ggccgagcag gtcgaggtgc gcgccactcc tcccgccgtg gacacgacga gctcggggat
     4321 gggcgccaac ttgaacagcg acttcctggc gcggctgccg gtccagaggc agatggccag
     4381 cgtcgtgtac ctgtcgccgg gcgtcagcac gagcggcggg gccggcgagg cgaacccgtc
     4441 gatctcgggc gccagcggcc tcgagaacca gtacgtcgtc gacggcatca acgtcagcaa
     4501 caccgggtac ggcggcgtcg ggtcgtactc gatcgtgttc ggatcgctcg gcacgggcgt
     4561 gccgttcgac ttcatcgacg aagtacaggt gaaggttggc ggctacgagg ccgagttcgg
     4621 ccaggccacc ggcggcgtcg tgcaggccgt caccaagagc ggcgcgaaca ccttctcggg
     4681 cggcgtcttc ggctactggc agcccgagga gttcgaggcc gactaccgcg agacggtgct
     4741 gccgaacgcc acgcgggcga acgaggcggt caacacgcgc gaaacgttct tcagcgacgt
     4801 cggcgcgacg atcggcggcc cgatcgtccg cgaccgggtg ttcttcttcg gcgcggtcga
     4861 ccgctcgtgg gaacggaccg ggctgatcgc gcccgagggg ttcccgctgc gcgaccaggg
     4921 ccccgtggat cggaagcgga acatcacgtc gtactcggcc aagggaacct tccaggtggc
     4981 cgacgcgcac cgcttcgacg tttcggcctt cggcgatccc tccgagggca agagcgggcc
     5041 gcaacgccgc agcgcgctgc tcggcgagaa cctcacgtcc cgcttcagcg agctggactt
     5101 cggcggccac agccagacgg tgaggtacga ggggatcatc accccgtcgt tcctggtgac
     5161 ggggtccttc tcgcgggcga ccaacgccat cgaggagctg ccgtcgctca acgagtactc
     5221 catcatcgac acgacaacga ctccgaacct ccgcttcggc gggatcgggt tcttcgagaa
     5281 caacgagggc gagaacctgc agtaccaggc gaaggcgacc tatctcgtcg gcgaccacga
     5341 gttgcgcggc ggggtcctgt acgaggacat ctcgtacgac aacatcgtgg gacggaccgg
     5401 gcccaccttc gtcctgccga acggcgagcc gacggcgacc ggcgccagcg tcagcgtgtt
     5461 gcccgacccc aacttcggac ggatctaccg ggtcacgcgg gcgaacctga ccaacgtccg
     5521 ggcgaccgag cagcggtaca ccagtttctt cctgcaggac acgtggaagg tacatcccac
     5581 gctgacgatc aagccgggca tccgctacga gcagcagaag ctggtcggca acctggccga
     5641 tcaccagtgg gacggcaact gggccccgcg catcggagcg gtctgggacc cgacgggaca
     5701 gggccgcctg aagatcttcg gcaactgggg acgctactac gccaaggtgc cgaacgacct
     5761 cgccgcgagg gcgctctcgg ccgacgcggg cgcaacccgt gccgactact tcgacgagtc
     5821 gttgacgcag ccggtgcctg acggcgtcga ggcggccggt acaacccagc acctcctgct
     5881 ggcgggcctg agccccgcgg acttcgaccc ggactcgaag tccacctact ccgacgagtg
     5941 gctggtcggt ggcgaggtcg aggtcatccc ccagctcagc gttggcgtca actaccagca
     6001 ccggaacttc gggcggatcc tggaggacat cggccaggcg ccgatggtgg cgtacttcct
     6061 cgaaccgggg gaccagctgg cgagcgtgga gtacttcatc accaacccgg atgccaacac
     6121 gccggtggcg ttccaggaga agtacggcgc gtcgttcgag gaggcgatcc acgactacga
     6181 cgcggtgacc ttcaccctcg agaagcggtt cgccaaccgc tggggcctcc agtcgtcgta
     6241 ccggctggcc tggctcgagg gaaccttcga ggggttcttc cgcaacgaca acgggcagtc
     6301 cgacccggcg atcacctcgc tcttcgactt cccgaccaac gacccgagct acacgctgct
     6361 cggcgggccg ctcgggttcc ggggcgacat ccggttcctc ggcgcggcgg gccaggggcc
     6421 gctgccgctc gaccgccggc actcgatcaa ggtgtacggc aactacctct tcgacttcgg
     6481 gttgaacttc ggtgtcggga tgaccgcgct gtcgggcacg ccgctgacgg cgctcgcgtc
     6541 gaacccggtc tacgacaaca acggcgagat ccccgaaacg ccgcgcggtg ccggcttcga
     6601 gacggtggac ggcttcctcg accggacgcc gtgggacacc cggctcgacc tgcacgccga
     6661 ctactcgatc ccgctcggcc ggcagcggct gacgctgatc gcccaggtgt tcaacgtgtt
     6721 caaccggcgc gagccgttgg actacgacaa ctacaccgag gtcagcttcg gcgtgccgaa
     6781 ccccgacttc ggccgcgtca tcgcttacca ggcgccccgc agcctgcgcg tgggcgcccg
     6841 cttcgtgttc taggccgttc cagtgggggc gggccctccg gcccgccccg ctggaccgcc
     6901 ggcacggagg ccggcgctac tgggctgctc tcagctcggc aaggctcgtg tccacgcgcg
     6961 cgtcagggtt catcgtcttg cggatggcgt cccggagcgg catcccctcc ttcgacaggc
     7021 gctcgcgaag cgccgcgtcc gccgggaacc ggcgggcgcc ctcagtccag atctcgcgcg
     7081 ccttccccag atccgacagc cgccagtagt gcgcgtcgcc gagggccacc cacgcgcgcg
     7141 cgtgatgcgg ccgcggcgcc tcgagcgacc cgagctcgac ggcacgctcg agctcgg
//
LOCUS       DAOPQX010000183         7172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11562_length_7172_cov_4.190670, whole genome shotgun sequence.
ACCESSION   DAOPQX010000183 DAOPQX010000000
VERSION     DAOPQX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11562_length_7172_cov_4.190670"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKK95_10530"
     CDS             <1..742
                     /locus_tag="PKK95_10530"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOC18697.1"
                     /translation="ELGGIRRFVARGDTVVVKPNIGWDRSAEQGANTNPELVAALVTL
                     CREAGARKVIVTDVSCNEARVCFEHSGIAAAARAAGAEVMLPEERRFREANLGGDVLT
                     TWPVLEPLLTADKLINVPIAKHHSLTGCTLGMKNLYGIIGGQRSRLHQRIHESLADLL
                     AFARPTLTVLDAYRVMMRGGPTGGSLGDVELRRTLIAGTDPVAIDAYAAKAFWDLDHY
                     RLPFLRIAQARGLGKTSFEEVRSRVVQV"
     gene            745..2409
                     /locus_tag="PKK95_10535"
     CDS             745..2409
                     /locus_tag="PKK95_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC18698.1"
                     /translation="MKTHSRLPLLRRISQVAFFAAFVFLLLQTEFTGSFRGATGDTRL
                     NWPVSIFLEADPLAALTTALSTGTLYRGLLWSLVILIPTFLLGRFFCGWICPLGTLNH
                     FFGHLKSEKKRGRSLLESNRYKRWQAAKYYVLAVVLVAAAFGSLVAGFVDPIPLTVRS
                     LALSVLPGINYATGSLIDALYDSPWGPVRFAGDALQFLLQGSLAAFEQPHFRQGIFLG
                     LIFAGLLALNLRVTRFWCRALCPLGALLGLSSRWSLLHLHKDDKRCDQCNRCLTRCQG
                     GDDPIPGAPWRKAECHLCFNCLGQCPTSGLAFRFGKPPAAEGMRELPQLQRRKLLTGV
                     AAGAALVPLMRSTTGLAVEADSKRIRPPGALDERHFLERCIRCGACMKVCPNNALHPT
                     FMEGGLEAMWTPIVVPRVGYCEPNCVLCSTVCPTGAIWELTLAEKLGKTGNPEAPTPV
                     KIGTAFYDQGRCLPWAMATDCIVCEEWCPTSPKAIYLVDAEVVDAESNTKTVRRPHVD
                     AKRCIGCGACEYACPVKDRPAVYVTSVGETRSKTNQFILPAAPPRA"
     gene            2483..3664
                     /locus_tag="PKK95_10540"
     CDS             2483..3664
                     /locus_tag="PKK95_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOC18699.1"
                     /translation="MQVPAGFSRREFVKSLGAASAVTAGGLVLAGPGFGQAPPAAVET
                     NVADFMKVPRTPRSLPGLCPGRVVKVTDKRSLVNERFDGKVIDEMFRKGLTALTGKNP
                     KESFRLLIDPKDVVGIKVNPVGPPLINTRPEVVDAVIAWLVENGIPKSNIVIWDRFDY
                     MLTDAGFTRERFPGIAIEGLQTMDETGNKWRGPDGKHVSIDQFDQDVYYYVKGVVGKG
                     VKGYPDDEFYLNQHVFNGEYSYFGKLVTRKLTRIINVAAYKNTGASISMATKNLGYGA
                     LCNVGRLHAPLSLKASVEVLAAPVLRDKLALNITDGLRGQYDGGPDKNAQFVYDNHSL
                     YFATDPFALDMLCHKEINAKRKEMGAAVNESPRITEYLRYAESLGLGVVADEKMQVVN
                     A"
     gene            3703..4614
                     /locus_tag="PKK95_10545"
     CDS             3703..4614
                     /locus_tag="PKK95_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18700.1"
                     /translation="MRVSVAFFLLSLLGAGFPATAGARDFNAVQAKAAGDQALLPGEA
                     AVPGWKKDGAPRVFTQADLYGYIDGGAELFFEFGFDQLTLQKYKKGAHEVAVEIYRMT
                     DPVAAAGTYFMKRGKEARDPGFRERHTVSRHQLLFQRHRYYVTINNLGGADGLTGDLV
                     RFASAVASRLPADVPAAELKRLPTAGRVAGSERLFRGPVALQSLFTLGDGDILQQKGR
                     ITGVAADYRAEPGAKAPTTLLVVHYPSAAAAAQAMAFLQKNLDKYLERVSASPDRLVF
                     RDYEKKFGVAIVKGAAIEVRLHLPKAS"
     gene            5022..5510
                     /locus_tag="PKK95_10550"
     CDS             5022..5510
                     /locus_tag="PKK95_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOC18701.1"
                     /translation="MFDLLIVAATLLCALQAIRSPRLLMSALWLASASALTALLMYLL
                     GAPEVAVIELSVGAGLVTVLFVFAINIAGEEVTDLRALVPRPLAWLAALGAMGLGAWM
                     AIPGLRAPAGPFPEPAFAAVLWEQRGLDVLLQVVLIIGGVLGVLGLLAEGRVHARKEK
                     QS"
     gene            5507..5818
                     /locus_tag="PKK95_10555"
     CDS             5507..5818
                     /locus_tag="PKK95_10555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOC18702.1"
                     /translation="MTLSLPALAAAAVLGLLGIGVAGLLVTRNLIKVVVGIQVMTKGG
                     MLAMVLAGYLSGRVQTGAALALTVIVADTIVSVVALAFAVQVRRRFGTLDLQALSTLR
                     R"
     gene            5823..>7172
                     /locus_tag="PKK95_10560"
     CDS             5823..>7172
                     /locus_tag="PKK95_10560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOC18703.1"
                     /translation="MPSIETLAILSIAIPWLGALVVWLVRDHRPALQHGLSVAFALAG
                     GVAAVAMIPQRTEANVLGVALGSAFGELTFLPDGLGVFLAVVATVVGSLAVIFSVDYM
                     RGERQLGRYYALVLFFIGAMVGLVLSSNLLLVFVFWEITAFCSYALISFYNDDPKAVA
                     AGIKALIMTQIGGVGLLGGALLLYVYQGTYDVPTFLAHANALPAGVLAVMAFGFLVAA
                     AAKSAQFPFQTWLPDAMEAPTPISALIHAATMVNAGVYLLARFYPAFEGVPNWRLAVI
                     VVGAVTALLAALMAIVSNDLKRVLAHSTVSQLGYMVYAIGAGGVFASQFHLLSHSVFK
                     ALLFLSAGAVIHAVHTRDMREMGGLGREMPLVRAVMIVGALALAGIPVVNGFWSKELV
                     LEVGHEGGPAWAWAVMLMGAGLTALYTLRFVTMVFYGEPRGTRHVYDAGPAMKVALVP
                     "
BASE COUNT         1040 a         2461 c         2463 g         1208 t
ORIGIN      
        1 cgagctgggc ggcatccgcc gtttcgtggc caggggcgac accgtggtgg tgaaaccgaa
       61 catcgggtgg gaccggtccg ccgagcaggg ggccaacacg aacccggagc tggtggccgc
      121 cctggtgacg ctctgccgcg aggcgggcgc gcgaaaggtc atcgtcaccg acgtgagctg
      181 caacgaggcg cgcgtctgct tcgaacacag cggcatcgcg gcagccgcgc gggcggccgg
      241 cgccgaggtc atgctgcccg aggagcggcg ctttcgcgaa gcgaacctcg gcggagacgt
      301 gctgaccacc tggccggtac tggagccgct gttgaccgcc gacaagctca tcaacgtccc
      361 gattgccaag caccacagcc tcaccgggtg cacgctcggg atgaagaacc tctacggcat
      421 catcgggggg cagcgaagcc gcctgcacca gcgaatccac gagagcctgg ccgacctgct
      481 ggccttcgcc aggccgacgc tcaccgtgct cgacgcctac cgggtgatga tgcggggcgg
      541 cccgaccggc gggagcctgg gcgacgtcga gcttcgccgg acgctcatcg ccggcaccga
      601 tccggtggcc atcgacgcgt acgccgccaa ggccttttgg gacctcgatc actaccgcct
      661 ccccttcctg cggatcgcgc aggcccgcgg gctcggcaag acgagcttcg aggaggtcag
      721 gagccgggtg gtgcaggtgt gaccgtgaag actcattcca ggctgccgct gcttcgtcgc
      781 atctcgcagg tggcgttctt cgccgcgttc gtgttcctgc tgcttcagac cgagttcacg
      841 ggctcgttca ggggcgcgac gggcgacacg cgcttgaact ggcccgtctc gatcttcctc
      901 gaggccgacc cgctcgccgc cctgacgacg gcgctgtcga ccgggacgct gtatcgcggg
      961 ctcctgtgga gcctcgtcat cctcatcccg accttcctgc tggggcggtt cttctgcggc
     1021 tggatctgcc cgctcggaac cttgaaccac ttcttcggcc acctgaagtc ggagaagaag
     1081 cgcggcaggt cgctgctcga atcgaaccgc tacaagcggt ggcaggccgc gaagtactac
     1141 gtgctcgcgg tcgtgctcgt ggcggccgcg ttcggctcgc tcgtcgccgg cttcgtggac
     1201 ccgatcccgc tcacggtgcg ctcgctggcg ctttcggtgc tgcccgggat caactacgcg
     1261 accggcagcc tgatcgacgc gctctacgac agcccgtggg gtcccgtgcg tttcgccggc
     1321 gacgcgctcc agttcctcct gcaggggtcg ctggccgcct tcgagcagcc gcacttccgc
     1381 cagggcatct tcctgggcct gatcttcgcc gggctgcttg ccttgaacct gcgggtgacc
     1441 cggttctggt gtcgtgccct ctgccccctc ggcgcgctgc tcggcctctc ttcgcgctgg
     1501 tcgctgctcc acctgcacaa ggacgacaag cggtgcgacc agtgcaaccg gtgcctcacg
     1561 cgctgccagg gaggcgacga cccgatcccc ggcgcgccgt ggcgcaaggc cgagtgccat
     1621 ctctgcttca actgcctcgg ccagtgcccg acctcgggcc tggccttccg cttcggcaag
     1681 ccgcccgccg ccgagggcat gcgcgagttg ccgcagctcc agcgccgcaa gctgctgacc
     1741 ggcgtcgcgg ccggcgccgc gctggtgccc ctgatgcgct cgaccaccgg cctggcggtc
     1801 gaggccgact cgaagcgcat tcgtccgccg ggggcgctcg acgagcggca cttcctggag
     1861 cggtgcatcc gctgcggcgc ctgcatgaag gtctgcccga acaacgccct gcacccgact
     1921 ttcatggagg gcggcctcga ggcgatgtgg acgccaatcg tggtgccgcg cgtcggctac
     1981 tgcgagccga actgcgtgct gtgcagcacc gtctgcccca ccggggcgat ctgggagctg
     2041 acgctcgcgg agaagctggg aaagacgggg aaccccgagg cgcccactcc cgtcaagatc
     2101 gggacggcct tctacgacca gggccgctgc ctcccctggg cgatggccac cgactgcatc
     2161 gtctgcgagg agtggtgccc gacctccccc aaggccatct acctggtcga tgccgaggtg
     2221 gtggacgccg agtcgaatac gaagaccgtc aggcgccccc acgtggatgc caagcggtgc
     2281 atcggctgcg gcgcgtgcga gtacgcgtgc cccgtcaagg accgccccgc cgtctacgtg
     2341 accagcgtgg gcgagacgcg atcgaagacg aaccagttca tcctgcccgc ggcgccccca
     2401 cgggcgtagg gatcagggat cagggatcag gggtcgggaa gagagctgaa aggcggtgac
     2461 cgtgcgcaag ggctaccgtt ccatgcaagt tcccgccggc ttcagccggc gcgagttcgt
     2521 caagtcgctg ggcgcggcct cggccgttac ggcgggcggg ctggtgctcg cgggacccgg
     2581 tttcggccag gcgcccccgg cggcggtcga gaccaacgtc gccgacttca tgaaggtgcc
     2641 caggacgccg cgatcgttgc cgggcttgtg ccccggcagg gtcgtgaagg tcaccgacaa
     2701 gcggtcgctc gtcaacgagc ggttcgacgg caaggtcatc gacgagatgt tccgcaaggg
     2761 gctgacagct ctgaccggca agaatccgaa ggagagcttc cgccttctca tcgacccgaa
     2821 ggacgtcgtc ggcatcaagg tgaacccggt cggcccgccc ctcatcaaca cccgccccga
     2881 ggtcgtcgac gccgtcatcg cctggctggt ggagaacggg atcccgaagt cgaacatcgt
     2941 catctgggac cgcttcgact acatgctcac cgacgccggc ttcacccgcg agcgcttccc
     3001 gggcatcgcc atcgaagggc tgcagacgat ggacgagacg gggaacaagt ggaggggccc
     3061 ggacggcaag cacgtgtcga ttgaccaatt cgaccaggac gtctactact acgtgaaggg
     3121 cgtggtcggc aaaggcgtca agggctaccc cgacgacgag ttctacctga accagcacgt
     3181 gttcaacggg gagtactcgt actttggaaa gctcgtgacc aggaaactga cgcggatcat
     3241 caacgtcgcc gcctacaaga acacgggggc gtcgatctcg atggccacca agaacctggg
     3301 ctacggcgcc ctctgcaacg tcggccgcct gcacgcgccg ctgtcactca aggcaagcgt
     3361 cgaggtgctg gcggcgccgg tgctgcgcga caagctcgcg ctcaacatca ccgacggcct
     3421 gcgggggcag tacgacggcg ggcccgacaa gaacgcgcag ttcgtgtacg acaaccactc
     3481 gctgtacttc gccaccgacc cgttcgccct ggacatgctc tgccacaagg agatcaacgc
     3541 gaagcgcaag gagatggggg cggcggtcaa cgagagcccg cgcatcaccg agtacttgcg
     3601 ctacgccgaa tcgctgggcc tgggcgtggt ggcggacgag aagatgcagg tggtgaacgc
     3661 atagcgcatg gctgaagcca tgcggttgct ggaggtcacg gcatgcgtgt gtccgtcgcg
     3721 ttcttcctgt tgtcccttct tggcgccggg ttcccggcga cggcgggcgc ccgggatttc
     3781 aacgcggtcc aggcgaaagc ggccggcgac caggcccttc tgcccggcga ggcggccgtg
     3841 cccgggtgga agaaggacgg cgccccacgt gtcttcacgc aggccgacct ctacggctac
     3901 atcgacggcg gcgccgagct cttcttcgag ttcggcttcg accagctgac gctgcagaag
     3961 tacaagaagg gcgcgcatga agtggccgtc gagatctacc ggatgaccga cccggtggcc
     4021 gccgcgggca cctatttcat gaagcggggc aaggaggcac gtgacccggg cttccgcgag
     4081 cgccacaccg tcagccgcca ccagctgctc ttccaacgcc atcgctacta cgtcaccatc
     4141 aacaacctcg gcggcgccga cggcctgacc ggcgacctcg tgcggttcgc gtcggctgtg
     4201 gcgtcgcggc ttcccgccga cgtgccggcg gccgagctga agcgcctccc gacagccggc
     4261 cgcgtggccg gctcggagcg gctcttccgc ggccccgtcg ccctccagtc gctcttcacc
     4321 ctgggcgacg gcgacatcct ccagcagaag ggacgtatca ccggggtcgc agccgactac
     4381 cgggccgagc ctggagccaa ggcgccaacc accctgctcg tggtccacta cccctctgcg
     4441 gctgccgcgg cacaggcgat ggctttcctc cagaagaacc tcgacaagta tctcgagcgg
     4501 gtctcggcgt cccccgaccg actggtcttc agggactacg agaagaagtt cggcgtggcc
     4561 atcgtcaagg gcgcggcgat cgaggtgcgg ctccacctcc cgaaggcgtc ctgagcgcgg
     4621 ccggcgggaa cgcggctggc gctgcgttcc ccaacattcg tgcgcccgtc acccatcgcg
     4681 aacatgccgt gaaacgctgc acctgaactt tgttcactct tgtgaatcgt ttcacaaaag
     4741 gacttgtcct ttaccgttca gatcggtaag aatatccgcc ggacggacgc gggtcgggta
     4801 cttcggccac cattcatttt cgcggtttcg aaccggcttc atcgtctcgg tcggcttgtc
     4861 gtggctgtcg tcgcgggaag gctgctcggg atcctcctgc cgtctgccgc cgtccggtcc
     4921 ccgtcagtcg ccccgctgcg tccacaggcg cggcggcggc gctcgtcaac tggccccctc
     4981 gggatgcgca ggttctgcct cattctcctc aagggatctc catgttcgat ctgctgatcg
     5041 tagcagccac cttgctgtgt gcactgcagg cgatccggag cccgcggctg ctgatgtcgg
     5101 ccctctggct ggcgtcggcg agcgcgctga cggcgctcct gatgtacctg ctcggcgcac
     5161 ccgaggtggc ggtcatcgag ctgagcgtcg gcgccgggct cgtcacggtg ctgttcgtct
     5221 tcgcgatcaa catcgcgggc gaagaggtga ccgacctgcg ggcgctcgtg ccgcggccgc
     5281 tcgcgtggct ggcggcgctg ggggcgatgg gcctgggcgc ctggatggcg atccccggac
     5341 tgcgtgcacc ggccggcccg ttccccgagc cggcgttcgc cgcggtgctc tgggaacaac
     5401 gcgggctcga cgtgctcctg caggtggtgc tgatcatcgg cggcgtcctc ggcgtgctcg
     5461 ggctgctggc cgaagggcgg gtgcacgcgc gcaaggagaa gcagtcatga cgctctcgct
     5521 tccggcgctg gccgcggcgg ccgtcctggg gctgctgggg atcggcgtgg cgggcctgct
     5581 ggtcacgcgc aacctcatca aggtcgtcgt cggcatccag gtgatgacca agggaggcat
     5641 gctggccatg gtgctggcgg gctacctgtc gggccgcgtg cagaccggcg cggcactggc
     5701 gctcacggtg atcgtggccg acaccatcgt ctcggtcgtg gccctcgcgt ttgcggtaca
     5761 ggtgcggcgg cggttcggca ccctggacct ccaggcgctg tccacgctgc ggaggtaggg
     5821 cgatgccatc gattgagacg ctcgcgattc tctcgattgc catcccgtgg cttggcgcgc
     5881 tggttgtctg gctggtgcgc gaccaccggc cggcgctgca gcacggcctc tcggtcgcct
     5941 tcgccctggc cggcggcgta gcggccgtgg cgatgatccc gcagcggacc gaggccaacg
     6001 tcctcggcgt cgcgctcggc tcggcgttcg gggaactgac gttcctgccc gacggcctcg
     6061 gggtcttcct ggcggtcgtg gcgacggtcg tcggcagcct ggcggtgatc ttctcggtcg
     6121 actatatgcg gggcgagcgg cagctgggcc gctactacgc gctggtgctg ttcttcatcg
     6181 gcgcgatggt gggcctcgtc ctctcgagca acctgctgct ggtcttcgtg ttctgggaga
     6241 tcacggcgtt ctgctcctac gcgctcatct ccttctacaa cgacgacccg aaggccgtgg
     6301 cggcgggcat caaggcgctg atcatgacgc agatcggcgg cgtgggcctg ctcggcggcg
     6361 cgcttctgct ctacgtctac cagggcacgt acgacgtgcc gaccttcctc gcccacgcga
     6421 acgccctgcc ggccggcgtg ctggccgtga tggccttcgg cttcctcgtg gccgccgccg
     6481 cgaagtcggc gcagttcccc ttccagacct ggctgcccga cgccatggag gcgccgacgc
     6541 cgatcagcgc gctgatccac gcggcgacga tggtcaacgc cggcgtgtac ctgctggcgc
     6601 ggttctaccc cgccttcgag ggagtcccga actggcgcct ggcggtcatc gtggtcggcg
     6661 ccgtgacggc gctcctggcc gccctgatgg ccatcgtgtc gaacgacctc aagcgcgtgc
     6721 tcgcgcactc caccgtgagc cagctcggct acatggtgta cgcgatcggc gcgggcgggg
     6781 tgttcgccag ccagttccac ctgctgagcc actccgtctt caaggcgctc ctcttcctgt
     6841 cggccggcgc ggtgatccac gccgtccaca ccagggacat gcgcgagatg ggcgggctgg
     6901 gccgcgagat gccgctcgtg cgcgccgtga tgatcgtggg ggcgctcgcg ctggcgggta
     6961 tcccggtcgt caacggcttc tggagcaagg agctggtgct cgaggtcggc cacgagggcg
     7021 gcccggcgtg ggcctgggcg gtgatgctca tgggcgccgg cctgacggcc ctctacacgc
     7081 tgcggttcgt gacgatggtg ttctacggcg agccgcgcgg gacccggcac gtttacgacg
     7141 ccgggccggc catgaaggtc gcgctggtgc cg
//
LOCUS       DAOPQX010000184         7126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11649_length_7126_cov_4.809221, whole genome shotgun sequence.
ACCESSION   DAOPQX010000184 DAOPQX010000000
VERSION     DAOPQX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11649_length_7126_cov_4.809221"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3427)
                     /gene="dnaE"
                     /locus_tag="PKK95_10565"
     CDS             complement(<1..3427)
                     /gene="dnaE"
                     /locus_tag="PKK95_10565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOC18704.1"
                     /translation="MPEFIHLHLHTEYSLLDGACRVDELLDQAARQKVPALAVTEHGN
                     MFSSVVFHDKARKKGIKPILGCEAYVARGSRLSRGGTMQESNNHLVLLAETREGYENL
                     IKLVSAAYLEGFYYRPRIDKELLAQHAKGLIGLSSCLKGEIPWRLRGQQDREAIEAAA
                     TYRDILGPGNFFLELQDHGIEDQKAVNDGLLRVASALDLPLVCTNDVHYLHREDFRPH
                     DVLLCIGTGKTVSEAERMRYHGDQFYLKTPEEMAQVFGSLPGAMLNTVRIAERCDVDL
                     GPTRHQLPNFEVPGDTTLDEYFERMVWQGFERRLPRLERLAATGQLRHTIEEYRQRVA
                     YEIAMIQRMKYSGYFLIVWDFIRYAREHQIPVGPGRGSAAGSVVAYCLNITDVDPLHF
                     DLIFERFLNPERVSLPDIDIDFCERRRGEVIEYVTRKYGRENVAQIITFGTMKARAVV
                     RDVGRALSMPYAEVDKVAKQIPPALDMTLEKALAENPALKQLEASDPGVKELLQAARR
                     LEGMTRHASVHAAGVVIAPKAITEFAPLYKGARDEITTQWSMKEIERIGLLKMDFLGL
                     STLTLLDDAVKEIERTTGKRLDLAALPLDDAKTYELFSNGLTLGVFQFESSGMREILR
                     KARPQRFEDLIALNALYRPGPLRGGMVEDFINRKHGRVEVTYDLPQLEPVLRETYGVI
                     AYQEQVMRIANEVAGFTLGEADLLRKAMGKKDASVMQAQRQKFIDGAKQRGTSAKKAA
                     ALFDLMEAFAGYGFNKSHSTTYALLAYHTAYLKANYPAHFMAALLTIESQNTAKVTMY
                     LGECRELGVKVLPPDVNSSELAFTVVPEGVRFGLGAVKNVGEAAVLALLDARREEGGR
                     IRSLYKLCERVDSRHLNRRVLEALVKAGALDSLVAGPGGAPASPAIARARLFAAVEKA
                     LEHGNRSRRDRDQGQTQLFEIPGLSPEEEGSELPDAEPWPEARQLAAEKESLGLYWSG
                     HPIERYAGELRAIGARSLAELLASGGDGDEAGEPAEGTPRGGVEVAVGGIVAAVRPLK
                     TKKGDRMCAFTLDDPHGSIEVVVFPDAFGKHGAAVQTDAMVLVKGRFERDDETSRILA
                     AEVVPIEAVRERAARGVRIRLAMPPHDRGTVERLTEILMKHKGDRRVSLEIELR"
     gene            3502..4524
                     /locus_tag="PKK95_10570"
                     /pseudo
     CDS             3502..4524
                     /locus_tag="PKK95_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
     gene            complement(4746..5987)
                     /locus_tag="PKK95_10575"
     CDS             complement(4746..5987)
                     /locus_tag="PKK95_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOC18705.1"
                     /translation="MIEYDRFLSQSALKSQGSAIRKMGVMAIRPDMISFAPGYPDPVT
                     YAWPEFREIANELLAGGQSSVLQYGPTRGHKPFIEALVELLASRGIAATPDQVLVTTG
                     SQQGLDLAGRMLLDPGDVVLVELPAYTGAISAFRNTEARLAGVRQSPDGIDLDDLQAV
                     VARERAAGRRVRFLYLVPNFQNPTGLLLSREKRRLLLEWASRDDLLIVEDDPYGALYF
                     EDTASAEDTRPIKADDTEGRVIYLSSFSKTLAPGFRVAWLTAPEPLIAKLEVLKQTED
                     LLTPSLDQHIVYEAYRRGILTERLPMLRKFYQDKRTTMQRTLREELGDQVAWPEPKGG
                     FFLWVSLPVRCATDEMLPRAIEARVIYVAGSAFFVDGTGRNTMRLSFSLPSHDKIVEG
                     VRRLAKVVKAEIAGHPMPVEI"
     gene            complement(5996..6991)
                     /locus_tag="PKK95_10580"
     CDS             complement(5996..6991)
                     /locus_tag="PKK95_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18706.1"
                     /translation="MTHPGRARTALKRGALLVAANWQVVAIQSVADTTFKLLAAAPIL
                     GGALLATLLLDRDVGEILAGNLRDTLFRVAATLAAHPFALASYLVGFFTVVAGGVVLM
                     FLVKGGTVSVLVRADRVAGPIERQPLRMAGFRRGMQFSIEGFTRGASYLFRRYLVLGV
                     LLTGVYTVAAALCLVLAWGLYRLGAGDRPVLVGSVAALGFALFFVITTLVNVLYLLAQ
                     IALAVRDCGVARAIAEVPRFIRGETWKVMRVFLVTVLLLILATLLSLVAAWGFYLVAY
                     VPLAGIIVLPLQLGAWMLRSLLFQYLGLTALGAYLSLYRSFAGDRYSEGPSPADL"
BASE COUNT         1017 a         2530 c         2508 g         1071 t
ORIGIN      
        1 ggcgcagctc gatctcgagc gacacccggc ggtctccctt gtgcttcatg aggatctcgg
       61 tcagccgctc gacggtgccg cggtcgtggg gcggcatggc gaggcggatg cgcacgccgc
      121 gcgccgcccg ctcgcgcacc gcctcgatcg ggacgacctc ggcggcgagg atgcgcgacg
      181 tctcgtcgtc acgctcgaag cgtcccttca cgagcaccat cgcgtcggtc tgcacggcgg
      241 ccccgtgctt tccgaacgcg tcagggaaaa cgacgacctc gatgcttccg tgggggtcgt
      301 cgagcgtgaa ggcgcacatc cggtcgccct tcttcgtctt gagcggccga acggcggcga
      361 cgatcccgcc gacggcgacc tccaccccgc ctcggggtgt tccctcggcc ggctcccccg
      421 cctcgtcccc gtcgcccccg ctggccagca gctcggccag gctgcgggcg ccgattgccc
      481 tcagctcgcc ggcgtagcgc tcgatcgggt ggccgctcca gtagaggccg agcgactctt
      541 tctcggcggc cagctggcgc gcctcgggcc acggctcggc gtcgggcagt tcgctgccct
      601 cctcctccgg ggagaggccc gggatctcga acagctgcgt ctggccctgg tcgcggtcgc
      661 gccggctgcg gttcccgtgc tcgagcgcct tctcgacggc cgcgaacagg cgggcccggg
      721 cgatggccgg cgaggccggc gcgccgccgg ggccggcgac cagcgagtcg agtgcgccgg
      781 ccttgaccag cgcctcgagg acccggcggt tcaggtgccg tgagtccacg cgctcgcaca
      841 gcttgtacag cgaacggatc cgcccgcctt cctcgcgccg ggcgtcgagg agggcaagga
      901 cggccgcctc gccgacgttc ttcacggcgc cgaggccgaa gcgcaccccc tcgggcacca
      961 cggtgaaggc cagctcgctg ctgttcacgt cgggcggcag caccttcacg cccagctcgc
     1021 ggcactcccc gaggtacatc gtgaccttcg cggtgttctg cgactcgatc gtgagcaggg
     1081 cggccatgaa gtgcgccggg tagttcgcct tcaggtacgc ggtgtggtag gccagcagcg
     1141 cgtaggtggt ggagtgcgac ttgttgaagc cgtagccggc gaacgcctcc atcaggtcga
     1201 acagcgcggc cgccttcttc gcgctggtgc cccgctgctt cgcgccgtcg atgaacttct
     1261 ggcgctgcgc ctgcatgacc gaggcgtcct tcttgcccat cgccttgcgc agcaggtcgg
     1321 cctcgccgag cgtgaagccg gcgacctcgt tggcgatgcg catcacctgt tcctggtagg
     1381 cgatgacgcc gtaggtctcg cgcagcaccg gctcgagctg cggcaggtcg taggtgacct
     1441 cgacccggcc gtgcttgcgg ttgatgaagt cctcaaccat gccgccgcgg agcgggccgg
     1501 ggcggtagag ggcgttgagg gcgatgaggt cctcgaagcg ttgcgggcgg gccttgcgca
     1561 ggatctcccg catgcccgag ctctcgaact ggaacacccc gagggtcagg ccgttcgaga
     1621 acagctcgta ggtcttcgcg tcgtcgagcg gcagcgcggc caggtcgagg cgcttccccg
     1681 tcgtccgctc gatctccttc accgcgtcgt cgagcagggt gagggtgctg aggcccagga
     1741 agtccatctt gaggagaccg atccgctcga tctccttcat gctccactgg gtcgtgatct
     1801 cgtcgcgggc ccccttgtag agcggggcga actcggtgat cgccttgggg gcgatgacga
     1861 cgccggcggc gtggaccgag gcgtgccgcg tcatgccctc gaggcgcctg gccgcctgca
     1921 gcagctcctt cacgccgggg tctgacgcct cgagctgctt cagcgcgggg ttctcggcca
     1981 gcgccttctc gagcgtcatg tcgagggccg gcgggatctg cttggcgacc ttgtccacct
     2041 cggcgtaggg catgctgagc gcgcggccca cgtcgcgcac cacggcgcgc gccttcatcg
     2101 tcccgaaggt gatgatctgg gcgacgttct cgcggccgta cttccgggtg acgtactcga
     2161 tgacctcgcc gcggcggcgc tcgcagaaat cgatgtcgat gtcggggagc gacacccgct
     2221 cggggttgag gaaccgctcg aagatcaagt cgaagtgcag cgggtcgacg tcggtgatgt
     2281 tcaggcagta ggcgaccacg ctgccggccg ccgagccgcg cccgggaccg accgggatct
     2341 ggtgctcgcg ggcgtaccgg atgaagtccc agacgatcag gaagtacccc gagtacttca
     2401 tccgctggat catcgcgatc tcgtacgcga cgcgctgccg gtactcctcg atcgtgtggc
     2461 gcagctgtcc cgtggccgcc agccgctcga gccgcggcag ccggcgctcg aacccctgcc
     2521 acaccatccg ctcgaagtac tcgtcgagcg tggtgtcgcc gggcacctcg aagttgggca
     2581 gctggtggcg ggtcgggccg aggtccacgt cgcagcgctc ggcgatgcgt accgtgttga
     2641 gcatcgcccc gggcaggctg ccgaacacct gcgccatctc ctccggcgtc ttcaggtaga
     2701 actggtcgcc gtggtagcgc atccgctcgg cttcgctgac cgtcttgccg gtcccgatgc
     2761 acagcagcac gtcgtgcggg cggaagtcct cgcggtggag gtagtgcacg tcgttggtgc
     2821 agaccagcgg caggtcgagc gccgacgcga cgcggagcag cccgtcgttc accgccttct
     2881 ggtcctcgat gccgtggtcc tgcagctcga ggaagaagtt gccggggccg aggatgtcgc
     2941 ggtaggtcgc ggcggcctcg atcgcctccc gatcctgctg cccccgcagc ctccagggga
     3001 tctcgccctt gaggcagctg ctcaggccga tcagcccctt cgcgtgctgc gccagcagct
     3061 ccttgtcgat gcgcgggcgg tagtagaacc cctccaggta ggcggccgag acgagcttga
     3121 tgagattctc gtagccctcg cgggtctcgg cgagcagcac caggtggttg ttgctctcct
     3181 gcatcgtccc gccccgcgag aggcggctgc cccgcgccac gtaggcctcg cagccgagga
     3241 tcggcttgat tcccttcttc cgggccttgt cgtggaagac gaccgacgag aacatgttcc
     3301 cgtgctcggt caccgcgagg gccggcacct tctggcgggc cgcctggtcg agcagctcgt
     3361 ccacgcggca ggccccgtcg aggagcgagt actcggtgtg caggtggagg tggatgaatt
     3421 caggcatcaa cacggaaccg ggcgtggcgg tgattgccgg gacggcgcgg cggccggggg
     3481 ttcggaaggt attataagcg gatgccgacg cggcccgccg gcctcgcccc gctcgtcctg
     3541 ctgtcgctgg cgaccctggc ggccgcgcaa ggcgccccgc ccgctcgccc cgctcccgca
     3601 gccacccagc ggcctcccgc ggcggggacg ccgctctcgc ctcctgcaaa gggaacggcg
     3661 ctcgcgcctc cggcggcggc cgcgcccgcg ctcgcgctga ggagcgcgtg gaccaccgag
     3721 ctgccggcct ccccgtctgg cgcgcccgcc ttcgacgcgc gccacgcgta cgtgccgctg
     3781 cgcgacggcc ggttcacggc tgtcgggctc gagcgcggca gcgtgacctg gacggcagag
     3841 gcgcccgctg ccgcggtggc tgccggcgac gggctggtct tcctggccac gggggacgcg
     3901 gtcgaggcgc ggcgcgccgc cacaggtgag agggagtgga tctacgagcc cggagaggaa
     3961 ctcgcgccgg cgctcagctg ggacggcgga tggctgctgg cgtcaaccgt ggacggcgat
     4021 gcgctgatgc tgggcgcgcg cgacgggaag ctgctctggc gccaggcgct cgccgcgcgg
     4081 cccgcaacgc gtcccgcgat gaccgccgac cgcctctacc tcgcgctcga ggacggccgg
     4141 gtcgtcgcgc ggcagattgc cgacggctcg ggcatctggg agcggcgcct cggcggccgg
     4201 cccgccgctc tcagcgcgct tgacgaccgg ctcttcgtcg gcgccgacga caagtacctc
     4261 tactgcctcg ataccaagga cggcgaccag aaatggcgtt ggcgtaccgg cggggcggtg
     4321 acgggcacgc cggccgtggg tggcgatcgc gtctacttcg tggcgttcga caacgtcctg
     4381 cgagcgctcg atcgcggcaa cggccaccaa cggtggatgc gcccactcgg cttccgcccc
     4441 accggcggcc ccctcctcgt gaacggcctg ctgctggtcc cttccctctc cccggaactg
     4501 gcagtattca gggccgacac tggagcccgc gccggaacgg ttcccgccgg cgccgagccg
     4561 gtggcgacgc cccacgtgct gtatcccgcg tcggcagggc ccggcgaggt gacgctggtc
     4621 gtggtcgggc gcacggggag gatcgagcgt ctcggccccg ccccgccggt gcttcaggcg
     4681 aagcccatcc ccggggcgcc aatgtacctg atgcccgaca tgccgaacgt ggaaagtcga
     4741 ccccttcaga tctccaccgg catcgggtgg ccggcgatct ccgccttcac cactttcgcg
     4801 aggcggcgga ctccctcgac gatcttgtcg tgtgacggca gcgagaacga cagccgcatg
     4861 gtgttgcgcc cggtgccgtc cacgaagaag gcgctgcccg caacgtagat cacgcgcgcc
     4921 tcgatcgccc gcggcagcat ctcgtcggtc gcgcacctca ccggcagcga cacccagagg
     4981 aagaagccgc ccttgggctc gggccaggcg acctggtcgc ccagttcctc gcgcagcgtg
     5041 cgctgcatgg tcgtccgctt gtcctggtag aacttcctga gcatcggcag ccgctcggtc
     5101 aggatgccgc ggcggtaggc ctcgtagacg atgtgctggt cgagggacgg ggtcagcagg
     5161 tcctcggtct gcttgagcac ctcgagcttc gcgatcagcg gctcgggtgc cgtcagccag
     5221 gccacgcgga agccgggcgc gagcgtcttc gaaaagctgc tcaggtagat cacgcgcccc
     5281 tcggtatcgt cggccttgat cggccgcgtg tcctcggccg acgccgtgtc ctcgaagtac
     5341 agcgcgccgt acgggtcgtc ctccacgatg agcaggtcgt cgcggctcgc ccactcgagc
     5401 agcagccgcc gcttctcgcg cgagagcagc agcccggtgg gattctgaaa gttgggcacc
     5461 aggtacagga accgcacccg gcggccagcg gcccgctcgc gcgccaccac cgcctgcagg
     5521 tcgtcgaggt cgatcccgtc gggcgactgc ctcacgccgg ccagccgggc ctcggtgttg
     5581 cggaaggcgc tgatcgcccc ggtgtaggcg ggcagctcca ccagcaccac gtcgcccggg
     5641 tcgagcagca tccgcccggc gaggtcgagc ccctgctgcg agccggtggt gaccagcacc
     5701 tggtcgggcg tcgccgcgat gccgcgcgac gccagcagct cgaccagtgc ctcgatgaac
     5761 ggcttgtgcc cgcgcgtcgg cccgtactgg aggacgctgc tctgcccgcc ggccagcagc
     5821 tcgttggcga tctcgcggaa ctcgggccag gcgtaggtca ccgggtcggg gtaccccggc
     5881 gcgaacgaga tcatgtcggg ccggatcgcc atgaccccca tcttccggat ggccgatccc
     5941 tgcgacttca aggctgactg cgagaggaaa cggtcgtatt cgatcatggc ggcgctcaca
     6001 ggtcggcggg agacggccct tcggaatacc gatctcccgc gaacgatcgg tacagggaca
     6061 ggtaggcgcc gagcgccgtc agcccgaggt actggaacag caagctgcgc agcatccagg
     6121 caccgagctg gaggggcagt acgatgatac ctgccagcgg gacgtacgcg accaggtaga
     6181 agccccacgc agccaccagc gacagcagcg tcgcgaggat cagcaggagc acggtgacca
     6241 ggaagacgcg catcaccttc cacgtctccc cgcggatgaa ccggggcacc tcggcgatcg
     6301 cgcgggccac cccgcagtcg cgcaccgcca gcgcgatctg cgccagcagg tagagcacgt
     6361 tcaccaacgt cgtgatgacg aagaagagcg cgaagccgag cgccgccacc gacccgacca
     6421 gcacgggccg atcgcctgcc ccgaggcggt agagccccca ggccagcacc aggcagaggg
     6481 ccgccgctac ggtgtagacg ccggtcagca gcacgccgag caccaggtac cgccggaaca
     6541 ggtacgacgc cccgcgcgtg aatccctcga tcgagaactg catgccccgg cggaagccgg
     6601 ccatccggag cggctggcgc tcgattggcc cggccacccg gtcggcccgc accagtaccg
     6661 agacggtgcc ccccttgacc aggaacatca ggacgactcc cccggccacg acggtgaaga
     6721 agcccacgag gtacgaggcg agggcgaacg ggtgcgcagc gagcgtggcg gccacgcgga
     6781 acagcgtgtc gcgcaggttt cccgcgagga tctcgccgac gtcgcggtcg agcagcagcg
     6841 tggccagcag ggcgccgccg aggatcgggg ccgccgcgag aagcttgaag gtggtgtcgg
     6901 ccaccgactg gatggcgacg acctgccagt tggcggcgac gaggagtgcg ccgcgcttga
     6961 gggcggtgcg agccctgccg ggatgcgtca tgaggcgccg ggaccgaagg ccaactatac
     7021 agggctcagg gctcgcggct caaggcaccc gccttcgcca ggctccagca cccgcaccgc
     7081 cggcatccgc cttcgccaag cgctcgcttc gctcccgcct tcgcgc
//
LOCUS       DAOPQX010000185         7103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11698_length_7103_cov_4.182605, whole genome shotgun sequence.
ACCESSION   DAOPQX010000185 DAOPQX010000000
VERSION     DAOPQX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11698_length_7103_cov_4.182605"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKK95_10585"
     CDS             <1..444
                     /locus_tag="PKK95_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18707.1"
                     /translation="LSVEDPASQVIDTDLREVKVPDLTRPEVSLSTPAVFRAANAREF
                     RTLAADPAALPAASREFRRTERLLVRFEAYLPGEKPPEVTARVLNRSGQGMSDIPVKA
                     PESPGTPYQLDIPLAGFPAGDYLIEVKARGADGEATELVPMRVAG"
     gene            complement(450..3530)
                     /locus_tag="PKK95_10590"
     CDS             complement(450..3530)
                     /locus_tag="PKK95_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18708.1"
                     /translation="MLVASAVVLASTAAPRSVPAQTPFVPYFGKNLVHYANFNWHIYK
                     TDHFDIYYYPEIESHLERVAAYAESAYQHVSAELKHDLASKVPLIIFKTHVDFQQQNV
                     IPGAAQEGVAAFAESSQNRMLLPLDEPPDLLYRTIAHELVHIFEYDIIPQSLIRRTAP
                     LWVYEGLSDFLAGHWAPLDLATVRDAAVADIVPRMSEMDDYGGFTNPRLVYNLGHAAF
                     EFMESRWGREGIRQFLFALRKSVIGGGENAYEEAFKLKADEFDQQFEKYLKDRFKPFR
                     DKERPADYGRDVAPDPEKGPFMSALTAEPSPSGELMAIVTGNRSDREYDIVLVSAKDG
                     QVVRNLTSGFDQGMGFDYLSIPGTRWNTVPWLSWAPRGDRLAYVVRMGRNKGIILQNV
                     VTRKVEQRLELKDIHEPESPAISPDGRTVAFSGLRNARSDIFGYDLQTGAITNLTDDE
                     FFDYGPVYSPDGTFIVYLARVSGNEKLFRLDLATRKKTQLTFGTHDDSAARFLNDHTL
                     VFSSTAVDPAVSIDPEEARNGNIYNIWTLDLRTGELNQYTDALGANLSPVPVVDAKNN
                     RVAFVTYYKGDYGLYQMELAKPIKSAATADFGAPGPIIDFQAPLSHTLIAENKEKKGS
                     WQRLFLEGRPPINVGVTNNGDIFGGTAISFSDVLGDKRVSITAASIAQYRTFGASYLN
                     LSRRLQWALQGFSQTLFFYGQYSGLFYDPAYAPYVDRDLAVATRSIHGGSAFAIYPLD
                     SYRRIEFTGGLMRYSEKYNDETLEGIAGDYQEAIYGRRLFNRGTIAPLGVAFVQETTV
                     FREFGPLAGNTMRLAYEYAPKLGNTLSRQTFDVDLRYYKRLAGSALVALRARGFRSFG
                     DYPDFLFFGGQGEMRGYNYLEFLGQNAAFANVELRFPLIEAMLTPLGVMGGVRGVFFF
                     NVGGGWYDNTGYRFATSKAETFTPLIDVRANEDGTLTPIYGDPVSINGFRLRDGRASY
                     GIGLETFALGFPIHFDWAWRTTFNRSWEDLVFARQGGSSAFRKAKFSVWIGYDF"
     gene            3735..4313
                     /locus_tag="PKK95_10595"
     CDS             3735..4313
                     /locus_tag="PKK95_10595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOC18709.1"
                     /translation="MKKSYWLLLAFGVGLIVLSFTPLPDLVGLIGSGGARDGGAAAQG
                     SGGSGACPANAPEARLDFTMKDMNGADVKFADHKGKVVLLNFWATWCPPCKMEIPWFV
                     EFQEQYGGKGFQVLGVSVDDPPEKLPPFAREYGTNYPLLVGADREDVQEAFGPIFGIP
                     VTVIIGRDGRVCKRHLGPVGKEQFESEIKALL"
     gene            4370..4594
                     /locus_tag="PKK95_10600"
     CDS             4370..4594
                     /locus_tag="PKK95_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18710.1"
                     /translation="MLKGFTHAHLACGCRIAFREGVEGSPVTVIVEMKAPGCVSPLHV
                     RDLPLYDHREALRAPTRLLPAEEGEFEEEG"
     gene            complement(4768..4953)
                     /locus_tag="PKK95_10605"
     CDS             complement(4768..4953)
                     /locus_tag="PKK95_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18711.1"
                     /translation="MERKAFLLRLDPALLEALQRWANDEVRSLNGQIEFLLTRALRDA
                     GRAKVPDRLPKDSRGKE"
     gene            complement(5078..5956)
                     /locus_tag="PKK95_10610"
     CDS             complement(5078..5956)
                     /locus_tag="PKK95_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOC18712.1"
                     /translation="MIREKEMTASSGWVALWILLSLLVLLGVLIVGEARQGPDASPWV
                     VVLYSLVLSFVGFLLAGLFIVNPNEAKVLQLFGRYVGTVKVQGFRWANPLYTKRRISL
                     RIRNFESGRLKVNDIEGNPIEIAAVIVWRVVETAEASFEVDNYENFVHVQSEAALRNL
                     ATSYPYDAHDDDQTSLRGQTAKVAEHLKTEIQDRLAKAGVEVIEARISHLAYAPEIAA
                     AMLQRQQAGAIIAARQRIVEGAVGMVEMALARLSEKELLVLDEERRATMVSNLLIVLC
                     GERAAQPVINTGTLYT"
     gene            6447..6812
                     /locus_tag="PKK95_10615"
     CDS             6447..6812
                     /locus_tag="PKK95_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18713.1"
                     /translation="MRFSRLVLVGVLLVVALWTLLSGRCGRDTRRDIAVEEGRVVVTN
                     LTGQRWSDIDVWLNTWYRAQAPALEPKQRLEIPLRVFVNGHGQHFEAHLRGPVGIEVT
                     ALAADGSAITLTWGEGRRR"
BASE COUNT         1095 a         2333 c         2486 g         1189 t
ORIGIN      
        1 ctgtcggtgg aagatccggc gtcgcaggtg atcgacaccg acctgcgcga ggtcaaggtg
       61 cccgacctga cccgccctga ggtgtcgctc agcacgccgg ccgtcttcag ggccgccaac
      121 gcccgcgagt tccggacgct ggcggccgac ccggcggcct tgcccgccgc gtctcgcgag
      181 ttcagacgca ccgagcgcct gctggtgcgg ttcgaggcct accttccggg tgagaagccg
      241 cccgaggtca cggcccgcgt cctcaaccgc tcggggcagg gcatgtcaga catcccggtg
      301 aaggcccccg agtcgccggg gaccccttac cagctggaca tcccgctcgc cgggttcccg
      361 gccggcgact acctgatcga ggtgaaggcc cgcggcgccg acggcgaggc caccgagctg
      421 gtgccgatgc gggtggcggg gtaagcgcac tagaagtcgt acccgatcca caccgagaac
      481 ttcgccttgc ggaaggcgct gctgccgccc tggcgggcga agacgagatc ctcccagctg
      541 cggttgaacg tggtccgcca cgcccagtcg aagtggatgg ggaagccgag ggcgaaggtt
      601 tcgaggccga tcccgtacga cgcgcggccg tcccgcaggc ggaagccgtt gatgcttacc
      661 gggtcgccgt agatcggggt cagcgtcccg tcctcgttcg cacggacgtc gatgagcggc
      721 gtgaaggtct cggccttgga ggtggcgaac cggtagccgg tgttgtcgta ccagccgccc
      781 cccacgttga agaagaagac ccccctgacc ccgcccatca cgccgagcgg cgtgagcatc
      841 gcctcgatca gcgggaagcg cagctcgacg ttggcgaacg cggcgttctg cccgaggaac
      901 tcgaggtagt tgtagccgcg catctcgccc tgaccgccga agaacaggaa atcggggtag
      961 tcgccgaagc tcctgaagcc gcgggcccgc agcgccacca gcgcgctccc cgccagccgc
     1021 ttgtagtagc gcaggtccac gtcgaaggtc tggcgcgaca gcgtgttccc gagcttcggc
     1081 gcgtactcgt acgccagccg catggtgttc ccggcgagcg gcccgaactc gcgaaagacg
     1141 gtcgtctcct gcacgaaggc gacgccgagc ggcgcgatcg tgccgcggtt gaacagccgg
     1201 cggccgtaga tggcctcctg gtagtcgccg gcgatgccct cgagcgtctc gtcgttgtac
     1261 ttctcgctgt accgcatcag cccgccggtg aactcgatgc ggcggtagct gtcgagcggg
     1321 tagatggcga aggccgaccc gccgtggatg ctgcgggtcg ccacggcgag gtcgcggtcc
     1381 acgtagggcg cgtacgccgg gtcgtagaag aggcccgagt actgcccgta gaagaacagc
     1441 gtctgcgaga agccctgcag cgcccactgg aggcgccgcg agaggttcag gtacgacgcc
     1501 ccgaatgtgc ggtactgcgc gatcgaggcg gcggtgatgg agacgcgctt gtcgccgagg
     1561 acgtcgctga acgagatagc cgttccgccg aagatgtcgc cgttgttggt gacgccgacg
     1621 ttgatcggcg ggcgcccctc gaggaacagc cgctgccagc tgcccttctt ctccttgttc
     1681 tccgcgatga gcgtgtggct cagcggcgcc tggaagtcga tgatcggccc gggcgcgccg
     1741 aaatcggcgg tcgcggccga cttgatcggc ttggccagct ccatctggta gagcccgtag
     1801 tcgcccttgt aataggtgac gaaggcgacc cggttgttct tggcgtccac caccggcacc
     1861 ggcgagaggt tcgcgccgag ggcgtcggtg tactggttca gctcgccggt ccgcaggtcg
     1921 agcgtccaga tgttgtagat gttgccgttc cgggcctcct cggggtcgat cgacacggcc
     1981 ggatcgaccg cggtggagga gaacaccagc gtgtggtcgt tcaggaaccg cgcggcgctg
     2041 tcgtcgtggg tgccgaaggt gagctgcgtc ttcttcctgg tggcgaggtc gaggcggaac
     2101 agcttctcgt tcccgctcac ccgcgccagg tacacgatga acgtgccgtc gggcgagtac
     2161 accggcccgt agtcgaagaa ctcgtcgtcg gtcaggttgg tgatcgcgcc ggtctggagg
     2221 tcgtatccga agatgtcgct gcgcgcgttg cgcaaccccg agaacgccac cgtccggccg
     2281 tcgggcgaga tggcgggcga ctcgggctcg tggatgtcct tcagctcgag ccgctgctcg
     2341 accttccggg tcaccacgtt ctgcaggatg atccccttgt tgcgccccat ccggacgacg
     2401 taggcgaggc ggtcgccccg cggcgcccac gagagccacg gcaccgtgtt ccagcgcgtg
     2461 cccgggatcg acaggtagtc gaaacccatg ccctggtcga agcccgaggt gagattgcgc
     2521 accacctggc cgtccttggc cgacaccagg acgatgtcgt actcgcggtc gctgcggttc
     2581 cccgtgacga tcgccatcag ctcgcccgag ggggacggct cggcggtgag cgcgctcatg
     2641 aaggggccct tctcggggtc gggcgccacg tcgcggccgt agtcggccgg acgctccttg
     2701 tcgcggaacg gcttgaagcg gtccttcagg tacttctcga actgctggtc gaattcgtcc
     2761 gccttcagct tgaacgcctc ctcgtaggcg ttctcgccgc ccccgatgac gctcttgcgc
     2821 agcgcgaaca ggaactgccg gatgccctcc cggccccagc gcgactccat gaactcgaag
     2881 gcggcgtggc cgaggttgta gacgagccgc gggttggtga agccgccgta gtcgtccatc
     2941 tcgctcatcc gggggacgat gtcggcgacc gccgcgtcgc gcacggtggc caggtcgagt
     3001 ggtgcccagt gcccggccag gaagtcggag agcccctcgt acacccacag cggcgccgtc
     3061 cggcggatga gcgactgcgg gatgatgtcg tactcgaaga tgtgcaccag ctcgtgggcg
     3121 atcgtccggt acagcaggtc gggcggctcg tcgagcggca gcagcatccg gttctgcgac
     3181 gactcggcga aggcagccac accctcctgg gcggccccgg ggatcacgtt ctgctgctgg
     3241 aagtcgacgt gggtcttgaa gatgatcagc ggcaccttcg acgccaggtc gtgtttcagc
     3301 tcggcgctga cgtgctggta ggcgctctcg gcgtaggcgg ccacccgctc gaggtgcgac
     3361 tcgatctcgg ggtagtagta gatgtcgaag tggtcggtct tgtagatgtg ccagttgaag
     3421 ttggcgtagt gaacgaggtt cttgccgaag tacggcacga acggcgtctg cgccggcacg
     3481 gaccgcggcg cggcggtcga ggcgaggacg acggcggagg cgacgagcaa cggggatagg
     3541 gctcgcctcg tgcgcatagg gtgtacctct ctcaaggggt caggccatcg atcttaccgc
     3601 cgtcacggcc gggaagccat cccgcgcctc agcaaatggg gtgccgtggg gcgcgagccg
     3661 gtgccaggcc tcgcgtcgcc ggcctgttac cttcgcgctc tgtagtaatc tctgtagatg
     3721 ggggtttcga gtcgatgaag aagtcgtact ggctgctgct tgccttcgga gtgggcctga
     3781 tcgtcctgtc cttcactccg ctgcccgacc tggtgggcct gatcggatcg ggcggtgcgc
     3841 gcgacggcgg ggccgccgcg caggggagcg gcggcagcgg ggcctgcccg gcgaacgcgc
     3901 ccgaggccag gctggatttc acgatgaagg acatgaacgg cgccgacgtg aagttcgccg
     3961 atcacaaggg gaaggtggtg ctcctgaact tctgggccac ttggtgcccg ccctgcaaga
     4021 tggagattcc ctggttcgtg gagttccagg agcagtacgg cgggaagggg ttccaggtgc
     4081 tcggcgtgtc ggtggacgat ccccccgaga agctgccgcc cttcgcccgc gagtacggga
     4141 cgaactaccc gctgctcgtg ggagcggacc gggaggacgt gcaggaggcg ttcggcccga
     4201 tcttcgggat cccggtgacg gtgatcatcg gccgcgacgg ccgggtctgc aagcgccacc
     4261 tcggcccggt gggcaaggag cagttcgaga gcgagatcaa ggcgctgctg tagtatgatc
     4321 gtttcgcggt gcgcggcggc tgtcgccgcg cgcgctgagg ttggagagga tgttgaaggg
     4381 gttcactcac gcgcatctcg cctgcggttg ccgcatcgcc ttccgcgagg gcgtcgaggg
     4441 cagccctgtc accgtcatcg tcgagatgaa ggcgcccgga tgcgtgtccc cgctgcacgt
     4501 gcgcgatctg ccgctgtacg accaccgcga ggcgctccgt gcgccgaccc gcctgctgcc
     4561 cgccgaggaa ggcgagttcg aggaggaagg ctagccgctc gcggcggaat gaggcggaac
     4621 gtagaattca aaaggtagaa atgcagaaag ggaggaggtg gcgcggagac cgcgtgacct
     4681 cctccctttt tcgttttcca ttccgcgtct tacgtttcac gcctactctt tccccctcga
     4741 gcctttcgga agttcgtccg gcaccgctta ctctttcccc ctcgagtctt tcggaagtct
     4801 gtccggcacc ttcgcccgcc cggcgtcgcg cagcgccctg gtgagcagga actcgatctg
     4861 cccgttgagg ctgcggacct cgtcgttcgc ccagcgctgc agcgcctcca gcagcgcggg
     4921 atccaggcga agaaggaaag ctttccgttc cacgacggcc tcaggggtcg ggagtaatta
     4981 gaagtcgtgc cgggtttccg acttcggcct caggggtcgg gagtaattag aagtcgtggc
     5041 gggtttccga cttctaatta ctcccgaccc ctctctgcta ggtgtacagc gtccccgtgt
     5101 tgatcaccgg ctgggccgcg cgctcgccgc acagcacgat cagcaggttg ctgaccatcg
     5161 tcgcccggcg ctcttcgtcg agcaccaaca gctctttctc cgacagccgc gccagcgcca
     5221 tctccaccat ccccaccgcg ccctcgacga tgcgctgccg cgccgcgatg atggccccgg
     5281 cctgctgccg ctgcagcatc gcggcggcga tctcgggggc gtaggcgagg tggctgatcc
     5341 gcgcctcgat gacctcgacg ccggcctttg ccagccggtc ctggatctcg gtcttcaggt
     5401 gctcggcgac cttggcggtc tggccccgca gcgacgtctg gtcgtcgtcg tgcgcgtcgt
     5461 aggggtagct ggtggcgagg ttgcgcaggg cggcctcgct ctggacgtgg acgaagttct
     5521 cgtagttgtc cacctcgaag ctggcctccg cggtctcgac gacgcgccag acgatcaccg
     5581 cggcgatctc gatcgggttg ccctcgatgt cgttcacctt caggcgcccg ctttcgaagt
     5641 tccggatccg gagcgagatg cgccgcttgg tgtagagcgg gttggcccac cggaagccct
     5701 gcaccttgac ggtgcccacg tagcggccga agagctgcag gaccttggcc tcgttcgggt
     5761 tgacgatgaa caggccggcg agcaggaagc cgacgaagga caggacgagc gagtacagga
     5821 cgaccaccca cggggaagcg tccggcccct ggcgggcctc ccccacgatg agaaccccca
     5881 ggagcacgag cagcgacagc aggatccaga gcgcgaccca gcccgacgac gccgtcatct
     5941 ctttctcgcg gatcatcgac gcacctcctc gccagtgcta gcagagtgat atcacattgg
     6001 tatcggactc gtcaatactg ccagtggcac agtggcacag gcgtcccgcc tgtgaggctg
     6061 ccagtggcac agacgtcccg cctgtgaggc tgccagtggc acaggcgtcc cgcctgtctg
     6121 gcggcagctc gcgggggcgc cgcggggccc ggctggcccc cggctcggac gcccgcccgc
     6181 cgcgagcgac gttgaggggc gggaaaccgc gccttccggg acctgtcggc ggaagcgcag
     6241 cttcgtaagc tgcgcggatt tcggccctca cggcgcggtt tcccatctga cgcccgcgac
     6301 gggcgtctcg cagcgggccg cgccggaccc cgctgcgccc cgcgatgccc tgtcactgcg
     6361 ccgggccgcg agcgccgctt gcccgcaacc gtggtcgtat tgggtggggc cgacgggtgc
     6421 ctcggcgcaa ctggtacact gaggcgatgc gcttctcccg gctggtcctc gtcggcgtcc
     6481 tgttggtcgt cgccctctgg acgctgctct cggggcggtg cggccgcgac acccggcgcg
     6541 acatcgcggt cgaggagggc cgggtcgtgg tgaccaacct gaccggccag cggtggtcgg
     6601 acatcgacgt ctggctcaac acgtggtacc gtgcgcaggc gcccgcgctc gagccgaagc
     6661 agcgcctgga gatcccgctc cgggtcttcg tcaacggcca cgggcagcac ttcgaagcgc
     6721 acctgcgcgg gccggttggc atcgaggtga ccgcgctggc ggccgacggg tcggccatta
     6781 cgctgacctg gggagaaggc cggcgacgct gacacaggcg agacgcctgt gcccctgaca
     6841 caggaacgag acgcctgtgc cactgacaca ggcgagacgc ctgtgcccct gacacaggcg
     6901 agacgcctgt gccactgaca caggaacgag acgcctgtgc cactgacaca ggcgagacgc
     6961 ctgtgccact gacacaggaa cgagacgcct gtgccgctga cacaggcgag acgcctgtgc
     7021 ccctgacaca ggcgagacgc ctgtgcccct ggccgggtcc gcgtggccga agggagacac
     7081 gaaatggcga agctgaagtc gga
//
LOCUS       DAOPQX010000186         7090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11731_length_7090_cov_4.405828, whole genome shotgun sequence.
ACCESSION   DAOPQX010000186 DAOPQX010000000
VERSION     DAOPQX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11731_length_7090_cov_4.405828"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="PKK95_10620"
     CDS             <1..564
                     /locus_tag="PKK95_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18714.1"
                     /translation="LAAGIDLVCFSGDKLLGGPQAGIVVGRRPLVDRVRRHPLMRALR
                     VDKLTYAALEATLGEYVAGRAGRTVPLLAMLRAEEGAVGARAERLAESLVPARRPPGT
                     AIEVIDGQSAIGGGTTPGLGIATRLLAIGLPQGPDWLDERLRRHDPPVIARIERDRLL
                     LDLRSVNPEDDAVVLAALNAALGSALP"
     gene            complement(686..2125)
                     /locus_tag="PKK95_10625"
     CDS             complement(686..2125)
                     /locus_tag="PKK95_10625"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOC18715.1"
                     /translation="MMFASDFAAIVPILCVIAAGIACLLAEALRQPGEQMPIGGLAAI
                     GLAGAGTTAALLWGRNAIGFGVITADSFALFVVLVLVAIGLLTVLFSPALIARDELPA
                     GEYYALVLFGLAGMMLMAMATDLLVIFLALEVLSLAVYVLTAIRRQAIGAEAGFKYFL
                     LGAFSSAFFLYGIAFAFGVSGSTKLDRLGSVVAAEAMNQSPLALVALGLLLVGFAFKV
                     SAVPFHMWTPDAYEGAPTLVAGFMSTAVKAAAFAAFTRVFLSALEPLRPSWAPALWAI
                     AAATMILGTVVGVAQTNLKRMLAYSSIAHAGYLLVALVAANDVGKSAVLFYLLVYGVG
                     NLGAFATIALLGTRDHANDDLDSFAGLSARHPAIAAAMAVFLLSLGGLPPTAGFVGKW
                     YVFSAAVQSGYYGLAVIGVLTSIVSIFFYLRVIVMMYMAPPAEVPAPAAPSRIAVAAL
                     AASVLITFYLGVLPGRVVELASRSVATIF"
     gene            complement(2122..3690)
                     /locus_tag="PKK95_10630"
     CDS             complement(2122..3690)
                     /locus_tag="PKK95_10630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOC18716.1"
                     /translation="MLTTLIFLPLAGAILLLLLKGREGRRDGLLRWLSLGVSLLVFAL
                     TLVVWARFDPSSADYQFVERREWIPAFGISYLVGVDGISLFLLVLTGFLTPIALLSSW
                     ASVHRRFAEFAAFLLALESAMLGVFVSLDLFLFYLFWDAMLIPMYFLIGMWGYERRIY
                     AAVKFILYTAAGSILMLVAIIGLGSLHYSATGEYSFELQALQEIPIPPNLEFWFFLAF
                     TLAFAIKVPLFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGLVRFSFPLFPDAALF
                     FAPYLAVLAVVGIVYGALVSMVQPDLKKLVAYSSVSHLGFVVLGITALNVQGVQGAVY
                     QMLAHGVSTGGLFLLVGMLSDRRHTRLIAEFGGLKAVMPRLVAAFMIITLASIGLPGL
                     NGFVGEFLIVLGAFRWDPRYAAVAVLGVILSAVYMLWMFQRVNYGTITEDENAALPDL
                     TRREAWVIGPTVAAAVLMGVLPGLFLRPMQPSVERLLQRVSREAVVTAPASSGAGLQA
                     GPVPHAPIRETAGR"
     gene            complement(3693..5669)
                     /gene="nuoL"
                     /locus_tag="PKK95_10635"
     CDS             complement(3693..5669)
                     /gene="nuoL"
                     /locus_tag="PKK95_10635"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOC18717.1"
                     /translation="MQISDPHMLLLIPLLPLAGFLVNAGIGRRLSKAASGGIAVGAMF
                     LSFAGALAASRALLRLPEDARVIDQALYTWISSGNLDVTLGLRLDPLAMVMILVVTGI
                     GALIHLYSTAYMHEETDAEYARYFSYLNLFAAFMLILVLAPNFAVMFVGWEGVGLCSY
                     LLIGFWYKKQSAADAGKKAFIVNRIGDAGFLLGMALLFSRFGTLDFAQLAREIAPLAP
                     ETAAFGALSAAALLLFVGATGKSAQLPLYVWLPDAMEGPTPVSALIHAATMVTAGVYM
                     IGRNAVLYTHAPGVLEIVAGVGVATALFAATIGLVQTDIKRVLAYSTVSQLGLMFLAM
                     GVGAFGAGIFHLYTHAFFKALLFLGAGAVIHALAGEQDLRRMGGLRRDLPVTYWTFLI
                     GALAIAGVPGFAGFFSKDEVLYRTFAAGYPLLWAAGAAASLLTAIYMFRLVFLAFHGE
                     ASDGRPQHGPHQHLHDAPPRMAVVLVALAAGAVLAGYVGVPAALGGSNRIEAFLEPSF
                     HPAAEASVEGAVEGLSHEAHDTGLELGLMGVSTAVALGGIALAALFFLRRRDLADAWA
                     ARYRGMHRLLLHKYYVDELYDAAIVQPVKTGSEQVLWKGVDAGLIDGAVNGAGLTIRG
                     ASASLRRLQTGSLRSYAASLFVGVLLVLGYLLWP"
     gene            complement(5698..6000)
                     /gene="nuoK"
                     /locus_tag="PKK95_10640"
     CDS             complement(5698..6000)
                     /gene="nuoK"
                     /locus_tag="PKK95_10640"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOC18718.1"
                     /translation="MVSLTHYLVLSAVLFAIGTAGVFLRRNLITVLLSIEVMLNAANL
                     SLVAFGRELGTVDGQVIVFFVVTVAAAEAAVGLALVIALFRLRESLDPDAYTSLKW"
     gene            complement(6070..6600)
                     /locus_tag="PKK95_10645"
     CDS             complement(6070..6600)
                     /locus_tag="PKK95_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOC18719.1"
                     /translation="MAPETLLFYALGAVAVAASLLVVGQRNPMYSVMLLIVSFLALAG
                     LYILLDAPFLAVIQIIIYAGAILVLFLFVVMLLNAEKEQEARAALGTVIGPRRAGAAL
                     GVILAAELGWAFWQMARGEGAGVETAARAPGADISTVRAIGQVLFTDYALAFEITSLL
                     ILVAMVGAVVLAKRHV"
     gene            complement(6590..>7090)
                     /locus_tag="PKK95_10650"
     CDS             complement(6590..>7090)
                     /locus_tag="PKK95_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOC18720.1"
                     /translation="LGNSLSLTEIVNAQAGTWFGVVPRWFVVLQPVGFLVYGISGIAE
                     TNRAPFDFPEAEQELVAGYHTEYSGMTFGMFFLAEYINMVTVSAVATSLFLGGWHGPF
                     LPESLGWIWFLLKVAALMFIYIWIRWTLPRYRYDQLMQFGWKILLPVAVLNLLATAAG
                     VIYFGA"
BASE COUNT         1323 a         2451 c         2488 g          828 t
ORIGIN      
        1 ctggccgccg ggatcgatct cgtgtgcttc agcggcgaca agctgcttgg cgggccgcaa
       61 gcggggatcg tcgtgggcag gcgtccgctc gtcgatcgcg tacgccgcca ccccctcatg
      121 cgcgcactcc gcgtggacaa gctcacatac gcggccctcg aggcaaccct gggcgaatac
      181 gtcgcggggc gcgcggggag gacggtgccg ctcctcgcga tgctgcgagc cgaagaaggg
      241 gcggtcgggg cacgggccga aaggctggcc gagagtctcg tcccagcgcg ccggcccccg
      301 ggcaccgcca tcgaggtaat cgacggccag tcggccatcg ggggcgggac gacgccgggg
      361 ctgggaatcg cgacgcgcct gctcgcgatc ggcctgccgc aaggccccga ctggctggac
      421 gagcggctcc gccgccacga cccgcctgtc atcgcccgca tcgagcgtga ccggctgttg
      481 ctcgacctga gaagcgtgaa ccctgaagac gacgcggtgg tgctcgcggc gctgaacgcc
      541 gctctgggct cggccctccc ctgaagcggc ggccgcgcag gtaggcgccc gtagcgcaac
      601 tgcgcacccg tagcgcaggg cttcagccct gcggcattgc gttacggccc tgcgcgacga
      661 ggccggcagc ctcgagacct ggaagctaga agatcgtcgc caccgaccgg ctggccagct
      721 cgacgacccg cccgggcagc actcccaggt agaaggtgat cagtaccgat gcagcgaggg
      781 ccgcgaccgc gatccgcgaa ggagcggccg gcgcgggaac ctcggcgggc ggcgccatgt
      841 acatcatcac gatcacgcgg aggtagaaga agatcgagac gatgctggtg aggacgccaa
      901 tcacggccag cccgtagtag cccgactgca ccgccgcgct gaagacgtac cactttccca
      961 cgaagcccgc cgtgggcggc aggccgccga gcgagagcag gaacaccgcc atcgcggcgg
     1021 cgatcgcggg gtgtcgcgcc gagaggcccg cgaagctgtc caggtcgtcg ttggcgtgat
     1081 cgcgggtacc gagcagcgcg atggtggcga acgcccccag gttgccgacg ccgtagacga
     1141 gcaggtagaa gagcacggcg ctcttgccca cgtcgttggc ggcgacgagc gccaccagca
     1201 ggtagccggc atgggcgatg ctcgagtagg ccagcatgcg cttcaagttg gtctgcgcga
     1261 cgccgaccac cgtccccagg atcatcgtcg ccgccgcgat ggcccagagc gcgggggccc
     1321 agctgggacg cagcggctcg agcgccgaca ggaacacgcg cgtgaaggcg gcgaaggccg
     1381 cggctttcac ggctgtggac atgaagccgg ccaccagggt gggcgccccc tcgtaggcat
     1441 cgggcgtcca catgtggaac ggcaccgccg acaccttgaa cgcgaagccc accagcagca
     1501 ggcccagcgc gaccagcgcg agcggcgact ggttcatcgc ctcggcggcg acgaccgagc
     1561 ccagacggtc gagcttcgtg ctgcccgaga cgccgaacgc aaaggcgatc ccgtacagga
     1621 agaaggcgct ggagaaggcg ccgagcagga agtacttgaa gccggcctcg gcgccgatcg
     1681 cctggcgccg gatggcggtc agcacgtaga cggcgagcga cagcacctcg agcgcgagga
     1741 agatgaccag gaggtcggtg gccatcgcca tcagcatcat gccggccagg ccgaacagca
     1801 ccagggcgta gtactccccg gccggaagct cgtcgcgggc gatcagcgcc ggcgagaaca
     1861 gcaccgtgag cagcccgatg gcgacgagca ccagcacgac gaaaagggcg aagctgtccg
     1921 cggtgatgac gccgaagcca atcgcgttgc gcccccacag gagggccgcc gtggtgcccg
     1981 cgccggccag gccgatcgcc gccaggcccc cgatcggcat ctgctcgccc ggctggcgca
     2041 gcgcctcggc gagcaggcag gcgatgccgg cagcgatgac gcagaggatg gggacgatcg
     2101 cggcaaagtc gctggcgaac atcatcggcc tgccgtttcc cggatcggcg cgtgagggac
     2161 ggggcccgcc tgcaggccgg cgccggacga ggccggcgcg gtcaccacgg cctcgcggct
     2221 cacccgctgc aggaggcgct cgaccgacgg ctgcatcggg cgcaggaaga ggccgggcag
     2281 cacgcccatc aggaccgcgg ccgccacggt cggcccgatc acccacgcct cgcggcgggt
     2341 gaggtcgggc agcgccgcgt tctcgtcctc ggtgatcgtc ccgtagttga cgcgctggaa
     2401 catccagagc atgtagaccg ccgagaggat gacgccgagc accgccaccg cggcgtagcg
     2461 cgggtcccac cggaaggcgc cgagcacgat caggaactcc ccgacgaagc cgttgaggcc
     2521 gggcagcccg atggaggcca gcgtgatgat catgaaagcc gcgaccaggc ggggcatgac
     2581 cgccttgagc ccgccgaact cggcgatcag gcgcgtgtgc cggcggtcgg agagcatccc
     2641 caccagcagg aacagcccgc cggtgctgac gccgtgggcc agcatctggt acaccgcccc
     2701 ctgcactccc tgcacgttga gggcggtgat cccgagcacc acgaacccca ggtgactgac
     2761 gctcgagtac gcgaccagct tcttgagatc gggctggacc atcgacacca gcgcgccgta
     2821 gacgatgccg acgacggcga gcacggcgag gtacggggcg aagaagagcg ccgcgtccgg
     2881 gaacaacggg aaggagaagc gcaccaggcc gtaagtgccc atcttcagca ggacgccggc
     2941 gaggatgacc gacccggccg tcggcgcctc gacgtgcgcg tcgggcagcc aggtgtggaa
     3001 cgggaagagg ggcaccttga tggcgaaggc cagcgtgaag gcgaggaaga accagaactc
     3061 gaggttcggc gggattggga tctcctgcag cgcctgcagc tcgaagctgt actccccggt
     3121 cgccgagtag tggagcgagc ccaggccgat gatcgcgacg agcatcagga tgctgccggc
     3181 cgcggtgtac aggatgaact tcacggccgc gtagatgcgc cgttcgtagc cccacatccc
     3241 gatcaggaag tacatcggga tgagcatcgc gtcccagaac aggtagaaga ggaagagatc
     3301 gagcgagacg aacacgccga gcatcgcgct ctcgagcgcc agcaggaagg cggcgaactc
     3361 ggcgaaccgc cggtgcaccg aggcccagga cgagaggagc gcgatcggcg tcaggaaccc
     3421 ggtcagcacc agcaggaaca ggctgatgcc gtcgacgccg acgaggtagc tgatgccgaa
     3481 ggccgggatc cactcgcggc gctcgacgaa ctggtagtcg gcgctcgacg ggtcgaagcg
     3541 cgcccagacg accagggtga gggcgaacac cagcagcgac accccgagcg agagccagcg
     3601 gaggagcccg tcgcggcgcc cctcgcgccc cttcaggagc agcagcagga tcgcaccggc
     3661 gagcggcagg aagatcaggg tcgtcagcat acctacggcc acagcaagta cccgagaacg
     3721 agcaggacgc cgacgaagag cgaggcggcg tacgaccgca gcgagccggt ctgcagccgg
     3781 cggagagagg cgctggcccc tcggatggtc agccccgcgc cgttgacggc cccgtcgatc
     3841 agcccggcgt cgaccccctt ccagagcacc tgctcggaac ccgtcttcac cggctgcacg
     3901 atcgcggcgt cgtacagttc gtccacgtag tacttgtgga gcagcagccg gtgcatcccg
     3961 cggtagcggg cggcccacgc gtcggccagg tcgcggcgcc gcaggaagaa cagcgcagcc
     4021 agggcgatgc cgccgagggc aacggcggtc gagaccccca tcaggcccag ctcgaggccc
     4081 gtgtcgtgcg cctcgtgaga cagcccttcc accgcccctt cgacgctcgc ttcggccgcc
     4141 gggtggaagc tcggctcgag gaacgcctcg atgcggttgc tgccgccgag cgcggccggg
     4201 accccgacgt acccggccag caccgccccg gcggccagcg cgacgagtac gacggccatg
     4261 cgcggcggcg cgtcgtgcag gtgctggtgc ggcccgtgct gcggccggcc atcggaggcc
     4321 tcgccatgga aggcgagaaa caccaggcgg aacatgtaga tcgccgtcag cagcgacgcg
     4381 gccgcgcccg ccgcccagag cagcgggtag ccggccgcga acgtccggta gagcacctcg
     4441 tccttcgaga agaaacccgc gaacccgggt acgcccgcga tcgcgagcgc gccgatcagg
     4501 aaggtccagt acgtgacggg aaggtcacgg cgcagccccc ccatccgccg caggtcctgt
     4561 tcgccggcaa gggcgtggat caccgcgccg gccccgagga acagcagcgc cttgaagaag
     4621 gcgtgcgtgt agaggtgaaa gatcccggcg ccgaaggcgc cgacgcccat cgcgaggaac
     4681 atcagcccga gctgcgacac ggtcgagtac gccagcaccc gcttgatgtc ggtctggacg
     4741 aggccgatgg tcgcggcgaa cagcgcggtg gccaccccga cgccggcgac gatctcgagc
     4801 acgccggggg cgtgcgtgta gagcacggcg ttgcgcccga tcatgtacac gccggccgtg
     4861 accatcgtgg cggcgtggat cagcgccgag accggcgtcg gaccctccat cgcgtcgggc
     4921 agccagacgt agagcggcag ctgcgccgac ttgccggtgg ccccgacgaa cagcagcagg
     4981 gcggctgccg agagcgcgcc gaatgcggcc gtctcggggg cgagcggggc gatctcgcgc
     5041 gcgagctgcg cgaagtcgag ggtgccgaac cgcgagaaca gcagcgccat gccgagcagg
     5101 aacccggcat cgccgatccg gttgacgatg aacgccttct tgcccgcgtc ggcggccgac
     5161 tgcttcttgt accagaaccc gatgagcagg tacgagcaca gcccgacgcc ctcccacccc
     5221 acgaacatca ccgcgaagtt cggggcgagc accaggatca gcatgaaggc ggcgaacagg
     5281 ttcaggtacg agaaatagcg cgcgtactcg gcgtcggtct cctcgtgcat gtaggcggtc
     5341 gagtacaggt ggatcagcgc gccgatcccg gtgaccacca ggatcatcac catcgcgagc
     5401 gggtcgaggc gcaggcccag cgtcacgtcg aggttgccgg aggagatcca ggtgtagagc
     5461 gcctggtcga tcacccgcgc gtcctcgggc agccgcagca gcgcgcgcga ggcggccagg
     5521 gcccccgcga aggacaggaa catcgccccg acggcgatgc cgcccgaggc ggccttcgac
     5581 agccgccggc cgatcccggc gttcaccagg aaccccgcga gcggaaggag cggaatcagc
     5641 agcagcatgt gcgggtcact gatttgcatg tccccttcac ggtccactcg tctgcgatca
     5701 ccatttcagg ctggtatacg cgtcggggtc gagcgactcg cgcaggcgga acagcgcgat
     5761 gaccagcgcc aggcccaccg ccgcctccgc cgccgccacc gtcacgacga agaacacgat
     5821 cacctggccg tccacggtgc ccagctcgcg cccgaaggcg acgagcgaca ggttcgcggc
     5881 gttgagcatc acctcgatcg acagcagcac ggtgatgagg ttccgccgca ggaacacgcc
     5941 cgccgtcccg atggcgaaca gcaccgccga gagcacgagg taatgggtca gagaaaccat
     6001 gattgtctgt cggggcaggc ctggtgcctg ccctcgtgca cctctgcgag cgggcggcgg
     6061 ggccgccccc tacacgtgtc tcttcgccag caccaccgcc cccaccatcg ccaccaggat
     6121 cagcagcgag gtgatctcga acgcgagggc gtagtcggtg aacaacacct gcccgatggc
     6181 ccgcaccgtg ctgatgtcgg cgcccggagc ccgggccgcc gtttcgaccc cggcaccctc
     6241 tccgcgcgcc atctgccaga acgcccaccc cagctccgcg gccaggatca cgccgagcgc
     6301 cgcaccggct cgccgcggcc cgatcaccgt gccgagcgcc gcccgcgcct cctgctcctt
     6361 ctccgcgttc agcagcatca cgacgaacag gaagaggacg aggatggcgc cggcgtagat
     6421 gatgatctgg atgaccgcga ggaacggggc gtccagcagg atgtagagcc cggccagcgc
     6481 caggaacgac acgatgagca gcatgacgct gtacatcggg ttgcgctgtc cgaccaccag
     6541 cagcgaggcc gccaccgcga ccgcgccgag cgcgtagaag aggagcgtct caggcgccaa
     6601 agtagatcac ccccgcggcc gtcgccagga ggttcagcac ggccaccggc aacaggatct
     6661 tccacccgaa ctgcatcagc tggtcgtagc ggtaccgcgg caacgtccac cgtatccaga
     6721 tgtagatgaa catcagcgcc gccaccttga gcaggaacca gatccacccg agcgactcgg
     6781 gcaggaacgg gccgtgccag ccgccgagga acaggctggt ggcaaccgcc gagacggtga
     6841 ccatgttgat gtactcggcc aggaagaaca tgccgaacgt catccccgag tactcggtgt
     6901 gatagcccgc gaccagctcc tgctccgcct ccgggaagtc gaacggcgcc cggttggtct
     6961 cggcgatgcc cgagatcccg tagaccagga acccgaccgg ctgcaggacg acgaaccacc
     7021 tcgggacgac gccgaaccag gtgccggcct gcgcgttcac gatctcggtg agcgacagcg
     7081 agttgccgag
//
LOCUS       DAOPQX010000187         7073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11770_length_7073_cov_5.420775, whole genome shotgun sequence.
ACCESSION   DAOPQX010000187 DAOPQX010000000
VERSION     DAOPQX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11770_length_7073_cov_5.420775"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..452)
                     /gene="citD"
                     /locus_tag="PKK95_10655"
     CDS             complement(186..452)
                     /gene="citD"
                     /locus_tag="PKK95_10655"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HOC18721.1"
                     /translation="MEMAITRKASAGTMQSSDLVVEIEPSPTIEIEIESTVKKQFEHL
                     IRRRIETVLARHGVTGARVRVSDRGALDYAIEARVETALRRATQ"
     gene            complement(452..1489)
                     /locus_tag="PKK95_10660"
     CDS             complement(452..1489)
                     /locus_tag="PKK95_10660"
                     /inference="COORDINATES: protein motif:HMM:NF019824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18722.1"
                     /translation="MIPPALPLLTSRERDEARRLVEAQGLSYEACDDVVGIYEQGRLV
                     ATAGRAGEVLKLFAIDEAHQGGQTLGRLLDALTALARADGRGVLLVFTRPGQASSFAA
                     YNFRLLASGSEAALLEHGGGLEAYLARHAGLRRPGANGAVVVNGNPFTLGHLHLVETA
                     ASRVSTLYLFVVREDRSVFPFEARLAMAKAATSHLPNVVVLDTSRYAVSAATFPSYFL
                     RRTDRAARAQMEVDATLFAAHVAPAFSIERRLVGEEPHCATTAAYNAVLADVLPSHGI
                     TLEVVPRLADAAGAISASRVRSALQRADLEGASRLVPEATAAFLRSSGGEAVARRLAQ
                     GAEPGCESQRG"
     gene            1728..2864
                     /locus_tag="PKK95_10665"
     CDS             1728..2864
                     /locus_tag="PKK95_10665"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOC18723.1"
                     /translation="MAALLAAWLSQACGEDRTPSPPPGDGSPGAVVGTFTAADGTRFS
                     VEVVATGLEVPWSLAFAPDGRIFVAERPGRVRIVSGGQVEARPALTLTDVYAQGEAGL
                     LGLTLHPGFESNGLVFLVYTARRADGIPVNRVVRFRELNGALGEPAVLLDDIGAASIH
                     NGSRIKFGPDGLLYVTMGDAADASLAQDLASLNGKVLRLNEDGTTPRDNPFSSPVYSL
                     GHRNPQGIDWDPVTGALWETEHGQSALDEVNRVEAGRNYGWPQIEGAETRPGMEPPVL
                     FFEQTVAPSGAAFYRGERIPSFSGNLFFGALRGTHLHRIVLQAPGRTEVASHERLLDG
                     VFGRLRGVAMGNDGALYFSTSNRDGRGTPAPDDDRILRVVPAPQ"
     gene            complement(3191..6025)
                     /locus_tag="PKK95_10670"
     CDS             complement(3191..6025)
                     /locus_tag="PKK95_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOC18724.1"
                     /translation="MKRMVRRSSVGLIAVAVVFTTAAAVSADPPSRVARLNYIKGDVS
                     FRPATLEEWGPATLNHPLTTGDHLWTDANAWTEMHVGSTAIRMAPQTAFAFLNLDDRL
                     VQMRVSEGAVDVRVRHLTGDVEINTPSAAIVVRRPGTYRVEVAPDEGATRVTVREGEA
                     EVYADEAPFSVFEGQTASLYGTDQVSHEIYRAEPADEWERWCRARDNREDRSLSLRYV
                     SYEMTGYEDLDEYGTWTSLPDYGWAWRPRYVAAGWAPYRDGNWRWVDPWGWTWIDYAP
                     WGFAPFHYGRWAHHHGGWYWVPGAYIARPIYAPALVAFIGGRNWGLSFGFGFHNALAW
                     FPLGPREFWYPHYHASHTYIRNLNITHVNVRNVNITNYNVSRGTYVNRAVVGAVTAVP
                     RDAFIGGRAVPRSAFAVDRNAVMRSEVVATSAPVAPRVESVLPRGGGRVPQPTASALR
                     RTVVARNEPAGAPVPFRARERALQANDGRPLDDNALSALRATSRASSAAGPVRLAGAR
                     ASRRGEVVNAGAGGNPAASRPASQGDRGGALAVPRGGERTRSSGGGRADRPGTASPSS
                     STSGREVNDRPAWARPRGGGAGTEATTPRSYGSGRNEAAPGGARSRGEAGGAVTGSPG
                     GGANDRPGAVVGGARSRDQAGGARNAPARGGDNDRPPSAAGSRGGAGSPGPAVGGARP
                     RGGGGESRPSGGAGSAPQSRPSPSSRPPGDVGGARPRSPGGQTMQMRSAPSGRATSPW
                     DGNDRPAASSSYRSTPQYRADTNRSLQYRSSAPERQYRSTPQYRSSAPSSAPRATPQY
                     RSSAPERQYRSTPQYRSSAPSSAPRATPQYRSSAPERQYRSTPQYRSSAPSSAPRATP
                     QYRSSAPSSAPRVRSQPQGRSSAPSVGRPSGGSRMPSFGGGARPSGSGGGSVSRPSGG
                     SRGGGSSAGTRSSGGTRSSGGGGARPRNRG"
     gene            6281..>7073
                     /locus_tag="PKK95_10675"
     CDS             6281..>7073
                     /locus_tag="PKK95_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOC18725.1"
                     /translation="MTIRRLLPALMLVPAVGIGSAVQVLALPQAPPAQTAQPAPKAAP
                     PAAKPATQKPRASQAARGTITFFITSPEGKPIPEATISMSGPATREGSTNKDGVVRLQ
                     AVRTGNYRVRAEAEGFISLERDVTMRNGLEVEMTLSPAPEPPEPPAPAAPPQAAEAPR
                     PAVAPDPNATLQLSSVVEFFSKNRLGRNEPRRESVVGRSGQATSSLLQVRDTVEGRTN
                     PSADEVLYVINGKAQVNSKGRIYEIETGSLILVPRGVTYSIANRGR"
BASE COUNT         1040 a         2594 c         2485 g          954 t
ORIGIN      
        1 caggcgagac gccatcacgg acaggcgaga cgccatcacg gacaggcgag acgccgtcac
       61 ggacaggcga gacgccatca cggacaggcg agacgccatc acggacaggc gagacgccgt
      121 cacggacagg cgagacgcca tcacggacag gcgagacgcc atcacggaca ggcgagacgc
      181 ctgtcctact gggttgcgcg ccgaagcgcc gtttccaccc gcgcctcgat cgcgtagtcg
      241 agcgcaccgc ggtcgctgac gcgcacccgg gctcccgtca cgccgtgcct ggccagcacg
      301 gtctcgatcc ggcgccggat caggtgctcg aactgcttct tcaccgtgga ttcgatctcg
      361 atctcgatgg tcggggacgg ctcgatttca accaccaggt cgctcgactg catggtgccg
      421 gcgctggcct tccgcgtgat cgccatctcc atcaccctcg ttgactctcg cagcccggct
      481 ccgcgccctg cgccagccgc cgcgcgaccg cctccccgcc ggacgagcgg agaaaggccg
      541 ccgtggcctc gggcacgagc cgcgacgccc cctcgagatc tgcccgctga agcgccgagc
      601 gcacccgcga cgcgctgatc gcgccggccg cgtcggccag gcgggggacg acctccagcg
      661 tgatcccgtg agagggcagc acgtcggcca gcacggcatt gtacgccgcg gtcgtcgcgc
      721 agtggggctc ttcgcccacc aggcgccttt cgatcgagaa cgcgggcgcc acgtgcgccg
      781 cgaagagtgt cgcgtccact tccatctgcg cccgggccgc ccgatccgtc cgccggagaa
      841 agtacgacgg gaaggtggcg gcgctgacgg cgtaccgcga cgtgtcgagc acgacgacgt
      901 tcggaaggtg ggaagttgca gccttggcca tcgccaggcg agcctcgaac gggaagaccg
      961 accggtcctc gcggaccacg aagaggtaca gcgtgctcac ccgcgatgcc gcggtttcca
     1021 ccaggtgcag gtgccccagc gtgaacgggt tgccgttgac gaccacggcg ccgttcgccc
     1081 caggccgccg cagtcccgcg tgccgggcga ggtacgcctc gaggccgccg ccgtgctcga
     1141 gcagcgcggc ctcggaaccg gaagccagca ggcggaagtt gtacgcggcg aagctggatg
     1201 cctggccggg ccgcgtgaag acgagcagca ccccgcggcc gtcggcgcgc gccagggccg
     1261 tgagcgcgtc gagcaggcgc ccgagcgtct ggccgccctg gtgcgcctcg tcgatcgcga
     1321 acagcttcag tacctcgcct gcccgcccgg ccgtcgccac cagccgtccc tgctcgtaga
     1381 tcccgaccac gtcgtcgcac gcctcgtacg agaggccctg cgcctcgacc aggcgccgcg
     1441 cctcgtcgcg ttcgcgggat gtcagaaggg gaagggcggg aggaatcatc gagccccgat
     1501 cgcggctgac gagtattacc gtgggaagca tgcaagtcaa accggtctcg ctggcgctcg
     1561 tgaaccggct catctacgac aagcgtcagt acgagctgcg ggcgcgagtg atggccctgc
     1621 tgttctgacc tacaatccgg tgagaggtgt cgatgccccc agacacgtcc accaggtgtg
     1681 cggccctccg cgcccgtcgc ttttcgagat catcatcctc cgcgatcttg gccgcgctgt
     1741 tggcggcgtg gctgtcccag gcctgcggcg aagaccgcac gccatcaccc ccgcccggcg
     1801 acggcagccc cggcgccgtg gtcggaacct tcacggccgc cgacggtacc cgcttctcgg
     1861 tcgaggtcgt cgccaccggc ctcgaggtgc cgtggagtct cgctttcgcg cctgacggcc
     1921 ggatcttcgt cgccgagcgc cccgggcgcg tgcggatcgt cagcggcggc caggtcgagg
     1981 cccgcccggc actgacgctc accgacgtgt acgcgcaggg tgaggcaggg cttctcgggc
     2041 tcacgcttca cccgggtttc gagagcaacg gcctggtctt cctcgtctac acggcgcgac
     2101 gcgccgacgg catcccggtc aaccgcgtgg tgcggttccg ggaactgaac ggggcgctcg
     2161 gcgaaccggc cgtgctgctc gacgacattg gcgccgcctc gatccacaac ggctcgcgca
     2221 tcaagttcgg ccccgacggg ctgctctacg tgacgatggg cgacgcggcc gacgcctcgc
     2281 ttgcccagga cctcgcctcg ctgaacggca aggtgcttcg actgaacgag gacggcacca
     2341 cgccgcgcga caacccgttc tcgtctccgg tgtactcgct cgggcaccgg aacccccagg
     2401 gcatcgactg ggacccggtc acgggcgcgc tgtgggaaac cgagcacggg cagtcggcgc
     2461 tggacgaggt caatcgcgtg gaggcgggac gcaattacgg ctggccccag atcgaagggg
     2521 ccgagacgcg gcccgggatg gagccgcccg tcctgttctt cgagcagacg gtcgccccgt
     2581 ccggcgccgc cttctaccgg ggtgagcgga tcccgtcgtt ctcggggaat ctcttcttcg
     2641 gcgcgctgcg ggggacgcac ctgcaccgca tcgtgctgca ggcgccggga cgcacggagg
     2701 ttgcctcgca cgaacgcctt ctcgacggcg tgttcggccg cttgcgcggg gtcgccatgg
     2761 ggaacgacgg tgccctctac ttctcgactt cgaaccgcga cgggcgcggg acgccggccc
     2821 cggacgacga ccggattctg cgagtcgtgc cggcgccgca gtgatcaatg gcacaaagac
     2881 cagtggcaca ggcgtctcgc ctgtgaggga gcaggcagtg gcagcggggc aaggaccagt
     2941 ggcacaggcg tctcgcctgt gagggagcag gcagtggcag caggacaaag atcagtggca
     3001 caggcgtctc gcctgtgagg gagcaggcag tggcagcagg acaaagatca gtggcacagg
     3061 cgtctcgcct gtgagggagc aggcagtggc agcggggcaa ggaccagtgg cacaggcgtc
     3121 tcgcctgtga ggcaggagga ggctcgccgt tcagctcaca ggcgggacgc ctgtgttgcc
     3181 actggctcgg tcagccccgg tttctcgggc gggcgccacc gccgcccgac gaccgcgttc
     3241 cgcccgacga ccgcgttccg gccgacgatc ccccgccccg gctgccgccg ctcggccgtg
     3301 aaacggaacc gccgcccgag cccgacggcc gggcgccgcc accgaacgat ggcatccggc
     3361 tgcctccgct cggacgcccc accgagggtg ccgacgaacg tccctgcggc tgcgaccgga
     3421 cccgcggtgc ggacgagggc gccgaggagc ggtactgcgg ggtggcccga ggtgcggacg
     3481 agggcgcgga cgaacggtac tgcggcgtcg agcggtactg ccgctcgggc gccgaggagc
     3541 ggtactgcgg ggtggcccga ggtgcggacg agggcgcgga cgaacggtac tgcggcgtcg
     3601 agcggtactg ccgctcgggc gccgaggagc ggtactgcgg ggtggcccgc ggtgcggacg
     3661 agggcgccga agagcggtat tgcggcgtcg agcggtactg ccgctcgggc gcggaagatc
     3721 ggtattgcag cgagcgattg gtgtcggctc gatactgcgg tgtcgaccgg tacgacgacg
     3781 acgccgcggg acggtcgttg ccgtcccacg gcgaagtcgc acggccggag ggagccgatc
     3841 gcatctgcat cgtctggccg cccggcgaac gcgggcgcgc gccgcccacg tcgcccggcg
     3901 gtctgctgga gggcgacggc cgcgactgcg gtgccgatcc cgcgccgccc gaggggcgtg
     3961 actcgccgcc tcccccccgg gggcgggcac ctcccaccgc tgggcctggc gagcctgcgc
     4021 ctccacggga tcccgccgcc gaaggcggcc ggtcgttgtc tcctccccgc gcgggggcgt
     4081 tccgcgcgcc gcccgcctgg tcacgcgacc gcgctccacc gacgacggct cccggccggt
     4141 cgttggcgcc gcctcccggt gagccggtca cggccccacc ggcctcgccc cgcgagcggg
     4201 cgccgcccgg cgcggcctcg ttccggccgc tcccgtagga tctcggcgta gtggcctcgg
     4261 tacccgcacc gccgccgcgt gggcgagccc acgccggccg gtcgttcacc tcgcggccgg
     4321 atgtcgacga gctgggggac gcggttccgg gacgatcggc ccgcccgccg ccgctcgacc
     4381 tcgtgcgctc gcctccccgg ggcacggcga gcgcgcctcc acgatcgccc tgcgaggccg
     4441 gccgggaagc ggccgggttg ccacccgcgc ccgcgttcac gacctcaccg cgccgcgagg
     4501 cccgcgcccc cgccagccgc accgggcccg ccgcgctcga ggcgcggctg gtcgcgcgca
     4561 gtgccgacag cgcgttgtcg tccagcggcc ggccgtcatt cgcctggagc gcgcgctcgc
     4621 gggcgcggaa gggcaccggc gcccctgccg gctcgttgcg cgcgacaacg gttcggcgca
     4681 aggcgctcgc cgtgggctgc ggcacgcgcc cgccgccgcg cggcagcacg ctctcgaccc
     4741 gcggcgccac gggcgcgctg gtcgcgacca cttccgaccg catcaccgcg ttccgatcga
     4801 ccgcgaacgc cgaccgcggg acggcccgcc ccccgatgaa cgcgtcgcgc ggcaccgcgg
     4861 tgaccgcccc caccaccgcg cggttgacgt aggtaccgcg cgacacgttg tagttcgtga
     4921 tgttgacgtt ccggacgttg acgtgcgtga tgttcaggtt ccggatgtac gtatggctcg
     4981 cgtgatagtg cgggtaccag aactcccgcg gccccagcgg gaaccaggcg agcgcgttgt
     5041 ggaagccgaa cccgaacgag agcccccagt tccgtccgcc gatgaaggcc accagcgccg
     5101 gcgcgtagat cggccgggcg atgtaggcgc cgggcaccca gtaccaaccg ccgtggtggt
     5161 gcgcccagcg gccgtaatgg aagggcgcga aaccccacgg ggcatagtcg atccaggtcc
     5221 agccccaggg atccacccag cgccagttgc cgtcacgata cggggcccag cccgcggcca
     5281 cgtaacgggg cctccacgcc catccgtagt cgggcaggga ggtccacgtg ccgtactcgt
     5341 ccaggtcttc gtaccccgtc atctcgtagg agacgtagcg cagcgacagc gaccgatcct
     5401 cgcggttgtc gcgcgcgcgg caccagcgct cccactcgtc ggccggctcg gcccggtaga
     5461 tctcatggga gacctggtcg gtgccgtaca gcgacgccgt ctgcccttcg aagaccgaga
     5521 agggcgcctc atcggcgtaa acctcggctt cgccttcacg aacggtcacg cgggtcgctc
     5581 cctcgtccgg ggccacctcg acccggtagg tccccggccg ccggaccacg atcgcggccg
     5641 agggcgtgtt gatctcgacg tcgcccgtca ggtggcgcac gcggacgtcc accgctcctt
     5701 ccgacacccg catctgcacg agacggtcgt cgaggttgag aaacgcgaag gcagtctgcg
     5761 gcgccatccg gatcgccgtc gagccgacgt gcatctcggt ccaggcgttg gcgtcggtcc
     5821 acaggtggtc gccggtggtg aggggatggt tcagcgtcgc gggcccccac tcttcgagcg
     5881 tggccggcct gaacgatacg tcccccttga tgtagttcag ccgggcaacc cgcgaaggtg
     5941 ggtcggccga cacagcggcg gccgtcgtga agaccacggc caccgcgatg agaccaaccg
     6001 aggagcgcct caccatccgc ttcatgatga cctccgctgc ctggccccct cacccggacg
     6061 gctgggagtg gctacctggg caagagcaag accagttcca ccgtgtctcg cacgctttgc
     6121 ccggaaaact ggcgcatttc cggcgccgcg atgctgcctg cgccgggtcg ccgccaatcc
     6181 gctgtcccct ccgccggacg cctgtcctgt cctcgtggcg taccccaagg cgtgccaagg
     6241 gcctacaatc agggggaatg tcccgagaga gggagacacg atgaccatca gacgattgct
     6301 gccagccctg atgctcgtgc cggccgtcgg cattggctcc gccgtgcagg tgctcgccct
     6361 tccgcaggcg ccgcccgccc aaaccgccca gcctgccccg aaggcggctc cgcccgcggc
     6421 caagccggcg acccagaagc cgcgggcgtc acaggcggcc cgcgggacca tcaccttctt
     6481 catcaccagc cccgagggaa agccgatccc cgaggcgacg atctcgatga gcggcccggc
     6541 cacccgtgag ggctcgacca acaaggacgg ggtcgtgcgg ctccaggcgg tgcggacggg
     6601 caactaccgg gtgcgtgccg aggccgaggg cttcatctcg ctcgaacgcg atgtcaccat
     6661 gcgcaacggc ctcgaggtcg agatgacgct cagccccgct cccgagcctc ccgagccgcc
     6721 ggctcccgcc gccccgcccc aggcagccga ggccccgcgg cccgccgtcg cgcccgaccc
     6781 gaacgccacg ctccagctct cgtcggtcgt ggagttcttc tcgaagaaca ggctggggcg
     6841 gaacgagccg cgacgcgagt cggtggtggg ccggtccggc caggccacct cctcgctgct
     6901 gcaggtgcgc gacacggtcg aggggcgcac caacccgtca gcagacgagg tgctctacgt
     6961 gatcaacggc aaggcccagg tgaacagcaa gggccgcatc tacgagatcg agaccggctc
     7021 gctcatcctg gtgccgcgag gggtgaccta ctcgattgcc aaccgcggcc gcg
//
LOCUS       DAOPQX010000188         7027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11870_length_7027_cov_4.492972, whole genome shotgun sequence.
ACCESSION   DAOPQX010000188 DAOPQX010000000
VERSION     DAOPQX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11870_length_7027_cov_4.492972"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..2390)
                     /locus_tag="PKK95_10680"
     CDS             complement(42..2390)
                     /locus_tag="PKK95_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOC18726.1"
                     /translation="MRDTKKKPSPRTRRGRRRVPKWAWWASLVLGIPAIAAAVVVGYY
                     YASFARLVDERLRGERERALPRVYARPLELRKGLTLTERQLVDRLNDLGYAYRATASK
                     PGEFAIDGLAVTIVPRGGDASGQLVRAVFAQPAVTKAGGSPRQPDRIDRIEAGGRAAD
                     AIALDPPLLTSMITGDREKRRLVRLARIPPHVVQAVLAVEDRRFYDHPGVDLIRIAGA
                     AFTNIFEDKPYLEGGSTLTQQLVKNVFLASVITNPKAKSYKRKLLEQFMAVVLERRAS
                     KDEILELYLNEVYLGQRGSFAIHGVAEGAKLFFGKDVANLTLPEAATLAGVIHSPYYL
                     SPFTAPERARERRNVVLRLMADAGFVAREAAEVASREELGIVARALEAEAPYFVDMIG
                     QQLAEQYPEFLQTSGRLDVYTSLDLHMQRVAQDAVREGLLAVDALLARRKRSGVPQAA
                     LLAVDARSGEILAWVGGRSYNQSQYNRVTTARRQPGSVFKPFVYLAAFEQAAEEGRAD
                     LTPATVVLDEPTTFLFGDEPYEPGNYQDEYDGPITLRRALAHSRNCATVRLAEVAGYD
                     TVAKFWKRFGTATSPKPYPSIALGAFEATPFEIATAYTVFPNLGEVRKLHAIRQISAD
                     GMEARPSPPPPQRVARATTSYLVENMLRSVINEGTGAAVRGSGFALDAGGKTGTTNDL
                     RDAWFVGFTAEILTVVWVGFDDNQALGLSGAQAALPIWTSFMTRALAGRPNTPLPEAS
                     DVVYVEIDRDNGKLAVPGCPRTFREAFAAGTEPLEPCELHRF"
     gene            complement(2466..4082)
                     /gene="bshC"
                     /locus_tag="PKK95_10685"
     CDS             complement(2466..4082)
                     /gene="bshC"
                     /locus_tag="PKK95_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HOC18727.1"
                     /translation="MPIDIRRLPWVRPLAADYAYDFERLAPFFAGNPADLAAWREAAK
                     RAGQHPRDRERLAAVIAAQQDRRQAPPEARRAAATLSDARAVAVVTGQQAGLFGGPLY
                     TLLKAVTAIKVAARVAGEVGVPAVPVFWIEAEDHDWNEVNACRVLDGEMRLQTVSLGT
                     PLGAGEGPVASVRLDPSIFAAVDILRSTLPATEFTDETLERLSAAYQPGFGMSEAFGR
                     WLESLLGRLGLVVYDASDPEAKPLAAGVFARELESPGESTRLALETGRALVERGYHAQ
                     VVPHADNVALFRLDGIRRTIHYRDGQFVIGDLETVPPGTLRGEAVSEPASFSPNVLLR
                     PIVQDALFPTVCYVAGPNELAYLAQLKPIYERFGVPEPLFVPRATATLLDSAGARFLA
                     RYDLPLEALQADDEAALNRLLEAQLPASVEEALRDASAAIDERMGRLVEAVPAVDPTL
                     ERTARSTLGKMQHDLRTLHDKIIHAAKRRDETLRRQFARTRGLAFPEGEPQERVVAQV
                     YFLNRYGDALVDRLREDLPIDMGTHWALTI"
     gene            4291..6492
                     /locus_tag="PKK95_10690"
     CDS             4291..6492
                     /locus_tag="PKK95_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC18728.1"
                     /translation="MVHQLGAGVLGPVFLGVDPEGDRQVAIKAFPLDITPERAATLAA
                     ALDRLVATPLGHPSLVALIAAGAEGSTAYLVQEYVLAESVDAAVREYGPAPVPDALRL
                     VGQLAGALDFAAVAGVHHGALHPRDVLVAPHEVRLTGLGIAGAFEEVGLRVTPRRPYA
                     APERRERADWGTPADVFSLAAVGFELLTGRRPGSVEALAADARSIPSPDPSALAEVFA
                     RALSPRPGDRYQTALAFAAALRQALTGEPIERPEAAPPAVASAALVAAAGAAAAGEEG
                     EGTDEAAEGEETEGAGEAAAGEEDEGTDEAAEGEEAEGADDVGAGLEPAAGEEAAAQA
                     EALSAVPPELEVFQSEPARDAVETRSLEFEAGQGGEVAAAVAPPQEDLAAGEPEIRNE
                     PALEAPAALPDAPVEAPIDELRSAEEISPQVAVPEGPSELGGETEPAAMAAPLLPLEV
                     EPERPSLPLEKRGAAEAWESPPIEKRSATEAWEPPPIEERTAEAWQVTPPPAPPFVPG
                     HPVAPPGVPRREAVMMHRSRRSGFPLSSLLAMLLLGLAAGFAGGYLVGRGEPGAGGSP
                     PATATQPASDAGSSEAPPTVVDRPELATPGAAGDRPAGNGAEAAPRDEARADEPPPPA
                     VPRPAEAAPRAAASRPVRTPAQSAGGRDEPEGPFEAPLLVISRPEGALVRLDGRPVGK
                     TPLRLTAIRAGAHVVRLELSGYLPWTAAIQVVSGAQNRVTASLERRPGGIE"
     gene            6494..>7027
                     /locus_tag="PKK95_10695"
     CDS             6494..>7027
                     /locus_tag="PKK95_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HOC18729.1"
                     /translation="MDSIDGSCATLALEDGTCYRGVACGAEGLAGGEVVFNTSMTGYQ
                     EILTDPSYSGQIVTMTCPQIGNYGTAEADHESEAPKAAGFIVRELSPIASNWRSTATL
                     GDYLARHGIVAIAGVDTRALTRKLRSSGVMRGVIATGRHETEELVARARALPSMEGAD
                     LVREVSCAAPFDFEPSGG"
BASE COUNT          965 a         2544 c         2595 g          923 t
ORIGIN      
        1 cccagcccct agcccctagc ccccaggccc tagcccctcg ctcagaaccg gtgcagctcg
       61 cacggctcga gcggctcggt gccggcggcg aaggcctcgc ggaaggtgcg cgggcacccg
      121 ggcacggcca gcttgccgtt gtcgcggtcg atctcgacgt acaccacgtc gctcgcctcg
      181 gggagcggcg tgttggggcg gccggccagg gcgcgcgtca tgaacgacgt ccagatcgga
      241 agcgcggcct gggcgcccga gagccccagc gcctggttgt cgtcgaagcc cacccacacg
      301 acggtgagga tctccgcggt gaagccgacg aaccaggcgt cgcgcaggtc gttggtggtg
      361 ccggtcttgc cgccggcgtc tagggcgaag cccgacccgc ggacggccgc gccggtgccc
      421 tcgttgatca cgctgcgcag catgttctcg acgaggtacg acgtggtggc ccgcgccacg
      481 cgctggggcg ggggcggcga gggcctcgcc tccattccgt ccgcgctgat ctgccggatt
      541 gcgtggagct tcctgacctc gccaaggttc gggaacacgg tgtaggcggt cgcgatctcg
      601 aacggggtcg cctcgaaggc gccgagcgcg atcgacgggt acggcttggg ggaggtcgcc
      661 gtgccgaagc gcttccagaa cttcgccacc gtgtcgtagc cggccacctc ggccagccgg
      721 acggtggcgc agttgcgcga gtgcgcgagc gcgcgccgca aggtgatcgg cccgtcgtac
      781 tcgtcctggt agttgccggg ttcgtacggc tcgtcgccga agaggaaggt ggtcggctcg
      841 tcgagcacca ccgtcgcggg cgtgaggtcg gcgcgccctt cctcggccgc ctgctcgaag
      901 gcggcgaggt agacgaacgg cttgaagacc gagcccggct gccgccgggc ggtcgtgacc
      961 cggttgtact gcgactggtt gtacgaccgc ccgccgaccc aggccaggat ctcgcccgac
     1021 cgcgcgtcca cggcaagcag cgccgcctgc ggaacccccg atcgcttgcg cctggccagc
     1081 agcgcgtcca ccgcgagcag cccctcgcgc acggcgtcct gcgcgacccg ctgcatgtgc
     1141 aggtcgagcg acgtgtagac gtcgagccgg ccgctggtct gcaggaactc cgggtactgc
     1201 tcggcgagct gctggccgat catgtccacg aagtacggcg cctcggcttc gagcgcccgc
     1261 gccacgattc ccagctcctc gcggctcgcc acctcggccg cctcgcgcgc gacgaacccg
     1321 gcgtcggcca tcagcctcag caccacgttg cgccgctcgc gcgcccgctc gggcgcggtg
     1381 aagggcgaca ggtagtaggg cgagtgaatc accccggcca gggttgccgc ttcgggcagc
     1441 gtcaggttcg ccacgtcctt tccgaagaac agcttcgcgc cctcggccac gccgtggatc
     1501 gcgaaggagc cgcgctgccc gaggtacacc tcgttcaggt acagctcgag gatttcgtcc
     1561 ttcgacgcgc ggcgctcgag gacgaccgcc atgaactgct cgagcagctt gcgcttgtac
     1621 gacttcgcct tcgggttggt gatgaccgac gccaggaaga cgttcttcac cagctgctgg
     1681 gtgagcgtgc tgccgccctc gaggtagggc ttgtcctcga agatgttggt gaaagcggcg
     1741 ccggcgatgc ggatgaggtc aacccccggg tggtcgtaga accggcggtc ctcgaccgcg
     1801 agcaccgcct gcaccacgtg cgggggaatt cgggccagtc gcacgaggcg gcgcttctcg
     1861 cggtcgcccg tgatcatcga ggtcagcagc ggcggatcga gcgcgatcgc gtcggccgcc
     1921 cggccccccg cctcgatccg gtcgatccga tccggctgtc gcggcgagcc gccggccttg
     1981 gtgacggcgg gctgcgcgaa gaccgcccgc accagctggc ccgacgcgtc gccgccccgc
     2041 ggcacgatcg tcaccgccag gccgtcgatc gcgaactcgc ccggcttcga ggccgtcgcg
     2101 cggtacgcgt accccaggtc gttcaggcga tcgaccagct ggcgctcggt gagcgtcaag
     2161 cccttccgca gctcgagcgg tctcgcgtag acgcgcggca gggcccgctc gcgctcgcca
     2221 cgcagccgct cgtccaccag gcgcgcgaag gaggcgtaat agtagccgac caccacggcg
     2281 gcggcgatcg cggggatgcc cagcaccagc gacgcccacc aggcccactt cgggacccgg
     2341 cgccggccgc ggcgggtgcg tggcgagggc ttcttcttcg tgtccctcac ggagctccga
     2401 ccagacgggg gcgagacggg gagtattcta cccgccttcg cccctcgggc tccggcgggc
     2461 acgcgtcaga tcgtcagcgc ccagtgcgtg cccatgtcga tgggcaagtc ctcgcgcagg
     2521 cggtcgacca gggcgtcgcc gtagcggttc aggaagtaca cctgggcgac gacccgctcc
     2581 tggggctcgc cctcggggaa cgccaggccc cgcgtccgcg cgaactgccg gcgcagcgtt
     2641 tcgtcgcgcc gcttggccgc gtggatgatc ttgtcgtgca gcgtccgcag gtcgtgctgc
     2701 atcttgccga gcgtcgagcg cgcggtgcgc tcgagcgtcg ggtcgacggc cggcaccgcc
     2761 tcgaccagcc gccccatccg ctcgtcgatc gcggccgacg cgtcgcgcag cgcctcctcg
     2821 accgaggccg gcagctgcgc ctcgagcagc cggttgagcg ccgcttcgtc gtcggcctgc
     2881 agcgcttcga gcggcaggtc gtagcgcgcg aggaagcggg cccccgccga gtcgaggagc
     2941 gtcgccgtcg cgcgcggcac gaacaacggc tcgggcacgc cgaagcgctc gtagatcggc
     3001 ttgagctgcg cgaggtacgc cagctcgttc ggccccgcga cgtagcagac cgtggggaag
     3061 agggcgtcct gcacgatcgg gcgcagcagg acgttcgggc tgaacgacgc cggctccgac
     3121 accgcctccc cgcgaagcgt cccgggcggc acggtctcga gatcgccgat tacgaactgc
     3181 ccgtcacggt agtggatcgt gcggcggatg ccgtccaggc ggaagagcgc cacgttgtcg
     3241 gcgtggggca ccacctgcgc gtggtagccg cgctcgacaa gcgcacggcc ggtctcgagc
     3301 gccagccgag tcgactcgcc gggcgactcc agttcgcggg cgaagacgcc ggccgccagc
     3361 ggcttggcct cggggtcgga ggcgtcgtag acgaccaggc ccaggcgccc gagcagcgac
     3421 tcgagccagc ggccgaacgc ctccgacatc ccgaacccgg gctgataggc ggccgacagc
     3481 cgctcgagcg tctcgtcggt gaactcggtg gccggaaggg tggaccggag gatgtcgacc
     3541 gcggcgaaga tcgaggggtc gagccgcacc gaggccaccg gcccctcgcc cgcaccgagc
     3601 ggcgtgccga gcgacacggt ctgcagccgc atctcgccgt ccagcacgcg gcacgcgttg
     3661 acctcgttcc agtcgtgatc ctccgcctcg atccagaaga ccgggacggc cggaacgccc
     3721 acctcgccgg ccacgcgggc cgccaccttg atggcggtga ccgccttcag cagcgtgtag
     3781 agcggcccgc cgaacaaccc cgcctgctgg cccgtcacca ccgccacggc ccgggcatcc
     3841 gacagcgtcg cggccgcccg gcgcgcctcg ggcggcgcct ggcggcggtc ctgctgcgcc
     3901 gcaatcaccg ccgccaaccg ctcgcggtcg cggggatgct gcccggcgcg cttggctgcc
     3961 tcccgccacg ccgccaggtc ggcggggttg cccgcgaaga acggggcgag ccgttcgaag
     4021 tcgtacgcgt agtcggccgc gagcggccgg acccacggca ggcgcctgat gtcgatcggc
     4081 acgcgacgcg acgatgtgga gggacgatcg gggtcgggtt gcaccggtag cctactctct
     4141 caggatgctc gcgcgcatgt caactgccgc cggcccttgg tcgcggcgcc cttcaatgct
     4201 agcatcaggt ggggcttcga ccgggagccg gcgctgacca gggacacgac cgtctcatcg
     4261 acgccgcccg ctggcttcgg gcgttttcgc gtggttcacc agttgggagc cggcgtgctc
     4321 ggcccggtgt tccttggcgt cgatcccgag ggcgaccgcc aggttgccat caaggcattc
     4381 cccctcgaca tcacccccga gcgggccgcg acgcttgccg ccgcgctcga ccgcctcgtc
     4441 gccacgcccc tcggccatcc ctcgctcgtc gcgctgatcg ccgcgggcgc cgagggcagc
     4501 accgcgtacc tcgtgcagga gtacgtcctc gccgagtcgg tggatgcggc ggtgcgcgag
     4561 tacggtcccg ctccggtacc cgacgcgctc cgcctggtcg ggcagctggc cggcgcactc
     4621 gacttcgccg cggtcgccgg cgtgcatcac ggcgcgctgc acccgcgcga cgtgctcgtc
     4681 gcgccccacg aggtgcgcct gacggggctt ggcatcgccg gcgccttcga agaggttggg
     4741 ctccgggtga cgccccggcg cccttacgcg gcgcccgagc gccgcgagcg cgccgactgg
     4801 ggaaccccgg ccgacgtctt ctccctggcg gcggtcggct tcgagctgct caccggccgg
     4861 cggccgggca gcgtcgaggc gctcgccgca gacgcgcgat cgatcccgtc gcccgacccc
     4921 tccgccctcg ccgaggtgtt cgcgcgcgcg ctgagcccgc gacccggcga ccggtaccag
     4981 acggcgctcg ccttcgcagc agcgctgagg caggcgctca cgggcgagcc gatcgagcgg
     5041 cccgaggcgg cgccgcctgc cgtcgcgtcc gccgcgctgg ttgcggcagc aggcgcggca
     5101 gccgcgggcg aagagggcga aggaacagac gaggcagcgg agggggaaga gacagaagga
     5161 gcaggcgagg cagccgcggg cgaagaggac gaaggaacag acgaggcagc ggagggggaa
     5221 gaggcagaag gagcagacga cgtaggggca ggactcgagc cagccgcagg agaggaggca
     5281 gccgcgcagg cggaggcctt gtccgcggta ccgccggagc tggaggtgtt ccagtccgag
     5341 ccggcgcggg acgccgtcga gacccgctcg ctggagttcg aggctgggca ggggggcgag
     5401 gttgcggccg cggtcgcgcc gccgcaagaa gacctcgcag cgggagagcc ggagatccgc
     5461 aacgagccgg cactcgaggc cccagcggcc ttgcccgacg cgccagtcga ggcgccaatc
     5521 gacgagttgc gctccgcgga ggagatttcc ccgcaggtgg ccgtccccga aggcccgagc
     5581 gagctgggag gggaaaccga gccagcggcg atggccgctc cgctgctgcc gctcgaggtc
     5641 gaacccgaga ggccgtcgct cccgctcgag aaacgcggcg cagccgaagc ctgggaatcg
     5701 ccgccgatcg agaagcgcag cgcaaccgaa gcctgggagc cgccgccgat cgaggaacgc
     5761 accgccgagg cctggcaggt gacgccgccg cccgcgcctc cgttcgtgcc gggccatccc
     5821 gtggcgccgc cgggtgtgcc ccggcgggag gccgtgatga tgcaccgttc gcggcggtcc
     5881 gggttcccgc tctcgtcgtt gctcgcgatg ctgctcctgg ggctggcggc cgggtttgcc
     5941 ggcggctacc tggtggggcg gggcgaaccg ggtgccggcg ggtcgccgcc ggcaaccgcg
     6001 acccagcctg ccagcgatgc ggggtcctcc gaggcgccgc cgaccgtcgt ggatcgtccc
     6061 gagctggcga cgcccggtgc cgccggggac cgtccggccg ggaacggtgc ggaagcggcg
     6121 ccgcgcgatg aggcgcgggc cgacgagccg ccgccgcccg ccgtgccgcg tccagcggag
     6181 gccgctccgc gggctgccgc gtcgcgcccg gtgcgcacgc cggcgcagtc cgccggcgga
     6241 cgcgacgagc cggagggccc cttcgaggcg ccccttttgg tgatctcgcg ccccgagggg
     6301 gcgctggtga ggctggacgg ccggccggtc ggaaagacgc cgttgcggct caccgcgatt
     6361 cgcgccggcg cgcacgtcgt ccgcctcgaa ctgagcgggt acctgccctg gaccgcggcg
     6421 atccaggtgg tgtcgggtgc ccagaaccgc gtgaccgcct ccctcgagcg gaggcccggg
     6481 ggcattgaat agattggaca gcatcgacgg gtcctgcgcg acactcgcgc tcgaggacgg
     6541 aacctgctac cgcggcgtcg cgtgcggcgc cgaaggcctg gccggcggcg aggtcgtgtt
     6601 caacacgagc atgaccggct accaggaaat cctcaccgat ccctcctaca gcggccagat
     6661 cgtcacgatg acgtgcccac agatcggcaa ctacggcacc gcggaggcgg accacgagtc
     6721 cgaggcgccg aaggcggccg ggttcatcgt gcgcgaactg tcgcccatcg cgagcaactg
     6781 gcgctcgacc gccacgctcg gcgactacct ggcccgccac gggatcgtcg cgatcgcggg
     6841 cgtggacacg cgggccctca cgcggaagct gcgatcgtcg ggcgtgatgc ggggggtgat
     6901 cgcgaccggg cggcacgaga ccgaggaact ggtcgcccgg gcccgcgcgc ttccctcgat
     6961 ggagggcgcc gacctggtgc gggaggtcag ctgcgcggcg ccgttcgact tcgagccgtc
     7021 ggggggc
//
LOCUS       DAOPQX010000189         7020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11882_length_7020_cov_3.982340, whole genome shotgun sequence.
ACCESSION   DAOPQX010000189 DAOPQX010000000
VERSION     DAOPQX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11882_length_7020_cov_3.982340"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PKK95_10700"
     CDS             complement(<1..1279)
                     /locus_tag="PKK95_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOC18730.1"
                     /translation="MTPPLPDRREFLKATGLFVFFLAEAPEALQEPARLPTGRQGYPT
                     DLNAYLHIKADGRVTCFSGKIEMGQGPATSLAQLLAEELDVPLASVDVVLGDTDLCPW
                     DMGTFGSLTIRQFGPLLREAAAEARAVLVQMASERLQVPAERLVVADGVVSDRDNRAA
                     RVTYAQLTEGRRIERRLGAKPALEPVERFTIVGRPARRRDAVEKVTGAARFAGDIVPP
                     GGALHARLLRPPAHGARLASVDVSAAEKRPGVTVVQDDDLVAVLHPHWDEADRALGDV
                     KSEFVRQDPPVDDRTIFDHLVKAAPAPRTAAEGGSLAEGEAQAAQMVERTWLDSYVAH
                     APIEPHAAVAAVEGGKVTVWAGTQTPFPLKSQIVQALKLPPEKVRVITPYVGGGFGGK
                     SASMQGLEAARLAVLTGKPVRVQWSREEEFFYDT"
     gene            complement(1276..1752)
                     /locus_tag="PKK95_10705"
     CDS             complement(1276..1752)
                     /locus_tag="PKK95_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOC18731.1"
                     /translation="MPVVSFTLNGKPARVEVDPDRALLWVLRDDLGLTGTKYGCGQAV
                     CGSCTVLLDDEPVRSCATRVSEVAGRRVLTIEGLADGDRLNPVQQAFVDHHAFQCGYC
                     TPGMILGAYALLKKNPRASRDEIVQALDPHLCRCGAHVRILQAVEQAGAAMRGGAR"
     gene            1867..3714
                     /locus_tag="PKK95_10710"
     CDS             1867..3714
                     /locus_tag="PKK95_10710"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC18732.1"
                     /translation="MRTRTVFLVALGLACSSIAPLAQMPPQQRLTPAEVQKALCLVEK
                     PQPVPQKYKVGFDAITPRATMAMLAYVASDGMEGRDTGSRGYALASEYAASLFKMWGV
                     APAGDLPPAVPPGRAMFGAAAPQAPPERSYFQDITFKETSDVRSALTVETRSGEVVKS
                     RTFTAGLDYQAGTGAAETISAPVVFAGFGISEASIGFDELKGLDLAGKVVLVISDAPG
                     RNDPKSPFQVKELKEKYFPPPDAARARMIRRMMGQGGGFDKLAEIAKLKPAAILVVSS
                     DRADSQIYNELSAVRTPGDDRPIIDRPRTRLSLAGDAGGPMAAMMGAGATSVSITREM
                     ADAILAGTGKTIDELQRAIETSMKPSSTGLPGVTVTLDTTSKTKLVRCRNVLGVIEGS
                     DPSLKNEYFVIGAHFDHTGRAGDYIFNGADDNGSGSVGVMAIARAMALNPVKPKRSIV
                     FALWTGEEKGLLGSRYYVRHPAFPIEKTVGYLNYDMISRAFDGPTLDRSTRQYEVDGT
                     AELVKRIRGDHFATVNLTAGTDFIPLTREMNKYVGLDLALRENPPGVGMGGSDHASFA
                     SVKVPYVYYMTAMTADYHQPSDSVEKVSPELFTKVVRMGYLTAFAYADR"
     gene            complement(3757..4185)
                     /locus_tag="PKK95_10715"
     CDS             complement(3757..4185)
                     /locus_tag="PKK95_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18733.1"
                     /translation="MIAARSETNDRSVTLPTAREARMWLVTTFGFYSVVEKAWDRERG
                     SLTVRARARKDLETLRQRYLPELGAIAEDESADYRFRAQAPREQVVEAFSRAVRDIDY
                     DNFKEAVLQRQGYARERIYHDAWHAFLAIQHPARRVGRGV"
     gene            complement(4294..4986)
                     /locus_tag="PKK95_10720"
     CDS             complement(4294..4986)
                     /locus_tag="PKK95_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOC18734.1"
                     /translation="MRRFHPGSWLLLTIALLTLAIPAAAQSNRPTVALLDFDFASIQH
                     WWSGNQDIGKGIADMIVDGLVEDGTYRVIERKRISDVLAEQDFSNSDRADPSQGQVVK
                     IGKALGVKYLIVGSVTKFGMEDKNTRFGGSVLGRRNFGVGDVGTKKGKASVAVTARMI
                     DTTTGEIMASAKGDATSSRSGLLLGGGANRVSGHIEFGSSNFRDTVLGEATEAAILQV
                     VEKLIAARNRLE"
     gene            complement(5156..5443)
                     /locus_tag="PKK95_10725"
     CDS             complement(5156..5443)
                     /locus_tag="PKK95_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18735.1"
                     /translation="MTLRPYWRRNGADAIALMRTALSEGPAMVERARRYDEELMRDLE
                     QAGGPKYAALAALAHRRAPGAQHGRRRVHQDARRPPELARKRQARKPAARR"
     gene            5611..6027
                     /locus_tag="PKK95_10730"
     CDS             5611..6027
                     /locus_tag="PKK95_10730"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HOC18736.1"
                     /translation="MSAMQQTARRFFEACETGKGWAECQQYCVPGATFAAQAEALAGV
                     STLEAYTEWMKGLFTPLPDGRYDLRSFAVDEERHHVVAYGVFHGTHSGEGGPVPPAGK
                     QVASDYVYIMEFDGDRIRHMTKVWNDAAALKQLAWM"
     gene            complement(6049..6567)
                     /locus_tag="PKK95_10735"
     CDS             complement(6049..6567)
                     /locus_tag="PKK95_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18737.1"
                     /translation="MRTAILAVLLAAFAAAPAHAQSIAEHARGVALDVLKDPTTYAPA
                     AASEIGKTLDWVSSQRFFAAGYVEANPHYTISGLPHDRPVSRATGHRLNLEVSLRVLG
                     YSAANNAINHAATRALSARFPQHKRLIRIASIVERAAFAVGTGYLNSAKNFRQWRKNV
                     NAPIQPVGALAW"
     assembly_gap    6680..6689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1051 a         2363 c         2517 g         1079 t
ORIGIN      
        1 aggtgtcgta gaagaactcc tcctcgcggc tccactgcac gcgcaccggc ttcccggtca
       61 ggaccgcgag gcgcgccgcc tcgagcccct gcatcgacgc cgactttcca ccgaagccgc
      121 ccccgacgta cggcgtgatg acgcgcacct tctcgggtgg gagcttcagg gcctgcacga
      181 tctggctctt gagcgggaac ggggtctgcg tgccggccca gaccgtgacc ttgccccctt
      241 cgacagcggc gaccgccgcg tgcggctcga tcggtgcgtg cgccacgtag ctgtcgagcc
      301 aggtgcgctc caccatctgc gccgcctgtg cctctccctc ggcgagcgac ccgccctcgg
      361 ccgcggttcg cggcgccggc gcggccttga ccaggtggtc gaagatcgtc cggtcgtcca
      421 ccggcgggtc ttgtcggacg aactccgact tcacgtctcc cagcgcccga tcggcctcgt
      481 cccaatgggg gtgcagcacc gcgacgaggt cgtcgtcttg cacgacggtc actccggggc
      541 gcttctccgc cgccgagacg tccacgctgg cgagccgggc cccgtgagcg ggcgggcgga
      601 ggaggcgcgc gtgcagcgca ccgccgggcg ggacgatgtc gccggcgaag cgggcggcac
      661 cggtgacctt ctccacggcg tcgcgccgcc tggccggccg tcccacgatc gtgaaccgct
      721 cgaccggctc gagcgccggc ttggcgccca gccgccgctc gatgcgccgc ccctccgtga
      781 gctgcgcgta ggtgacgcgc gccgcgcggt tgtcgcgatc gctgacgaca ccgtcggcca
      841 ccacgaggcg ctcggcggga acctggaggc gctcggaggc catctgcacg agcacggcgc
      901 gcgcctcggc cgcggcctcg cgcaacaacg ggccgaactg acgaatggtc agcgacccga
      961 aggtgcccat gtcccacggg cagaggtcgg tgtcgcccag caccacgtcc accgaggcga
     1021 gcggtacgtc gagctcctcg gccagcagct gcgccagcga ggtggcgggc ccctggccca
     1081 tctcgatctt cccggagaag caggtgacgc ggccgtcggc cttgatgtgg aggtaggcgt
     1141 tgagatcggt ggggtagccc tgcctgcccg tggggagacg ggccggctcc tgcagcgcct
     1201 cgggggcttc ggcgaggaag aagacgaaca ggccggtggc cttgaggaac tcccggcgat
     1261 cgggcagagg gggagtcatc gcgcgccgcc tctcatcgcg gcgccggcct gctcgaccgc
     1321 ctgcaggatc ctgacgtggg cgccgcagcg gcacaggtgc gggtcgaggg cctgcacgat
     1381 ctcgtcgcgg ctcgcgcgcg ggttcttctt cagcagtgcg tacgcgccca ggatcatccc
     1441 gggcgtgcag tagccgcact ggaacgcgtg gtgatccacg aaggcctgct gcaccgggtt
     1501 gaggcgatcg ccgtcggcga gcccctcgat ggtcaggacg cggcggccgg ccacctcgct
     1561 gacgcgcgtg gcgcacgacc gcacgggctc gtcgtccagc agcaccgtgc acgagccgca
     1621 caccgcctgg ccgcagccgt acttcgtgcc ggtcaggccg aggtcgtcgc gcagcaccca
     1681 gagcagggcg cggtcgggat cgacctccac ccgtgccggc ttgccattga gcgtgaagga
     1741 caccacgggc atgtgtgcgc tccaggtggg ggctgggaat cggtgggcag cagtataccg
     1801 ccctccgccc tcccttctgc tacacttcgc cgaccccccc gtcgcccccg gagagatccc
     1861 atccctatgc gaacgcgaac cgtcttcctc gtggcccttg gattggcctg ctcgtcgatc
     1921 gcgccgctcg cgcagatgcc tccgcagcag cggctcacgc cggccgaggt gcagaaggcg
     1981 ctctgcctgg tggagaagcc ccagcccgtg ccgcagaagt acaaggtggg cttcgacgcc
     2041 atcacgccgc gcgcgacgat ggcgatgctg gcctacgtcg cctccgacgg catggaaggc
     2101 cgcgacacgg gcagccgcgg gtacgccctg gcttccgagt acgccgcctc gctcttcaag
     2161 atgtggggcg tcgccccggc gggcgacctg ccgcccgcgg tgccgcccgg ccgcgcgatg
     2221 ttcggcgcgg ccgcgccgca ggccccgccc gagcggtcct acttccagga catcaccttc
     2281 aaggaaacga gcgacgtccg cagcgccctg acggtggaga cccgcagcgg cgaagtggtg
     2341 aagtcgcgca ccttcacggc aggtctcgac taccaggcag gtaccggcgc ggccgagacg
     2401 atctcggcgc cggtggtctt cgccggcttc gggatcagcg aggcgtcgat cggcttcgac
     2461 gagttgaagg ggctcgacct tgccggcaag gtcgtcctgg tgatctccga tgcgccgggc
     2521 aggaacgacc cgaagtcgcc cttccaggtc aaggagctga aggagaagta cttcccgccg
     2581 cctgacgcgg cgcgcgccag gatgatccgg cggatgatgg ggcagggagg cgggttcgac
     2641 aagctcgccg aaatcgccaa gctgaagccg gctgcgatcc tggtcgtctc cagcgaccgg
     2701 gccgactcgc agatctacaa cgagttgtcg gcggtcagga cgccaggcga cgaccggccc
     2761 atcatcgatc ggccgcgcac gcggctgtcg ctggccggcg acgcgggcgg gccgatggcc
     2821 gcgatgatgg gagccggcgc gacctcggtc tcgatcaccc gcgagatggc cgacgcgatc
     2881 ctcgccggca ccggcaagac catcgacgaa ctgcagcgcg cgatcgagac gtcgatgaag
     2941 ccctcctcga ccggcctgcc cggcgtcacg gtgacgctcg acacgacgtc gaagacgaag
     3001 ctcgtgcggt gccgcaacgt gctcggggtg atcgaaggat ccgacccgtc gctcaagaac
     3061 gagtatttcg tcatcggcgc ccacttcgac cacaccggcc gcgccggcga ctacatcttc
     3121 aacggcgccg acgacaacgg ctcgggctcg gtcggcgtga tggcgatcgc gcgggccatg
     3181 gcgctcaacc ccgtcaagcc gaagcgcagc atcgtcttcg ccctctggac gggcgaggag
     3241 aaggggctgc tcgggtcgcg gtactacgtg cgccacccgg cctttccgat cgagaagacc
     3301 gtggggtacc tgaactacga catgatcagc cgtgccttcg acggcccgac gctcgaccgt
     3361 tcgacacggc agtacgaggt ggacggcacc gccgagctgg tgaagcgcat ccgcggcgac
     3421 cacttcgcga ccgtgaacct gaccgcgggc accgacttca tccccctcac gcgggagatg
     3481 aacaagtacg tcggcctcga cctggcgctc cgcgagaacc cgcccggcgt cgggatgggc
     3541 gggtcggacc acgcgtcgtt cgcgtcggtg aaggtgccgt acgtctacta catgacggcg
     3601 atgacggccg actaccacca gccgagcgac agcgtcgaga aggtgagccc ggagctgttc
     3661 accaaggtcg tgcggatggg atacctgacc gcgttcgcgt acgcggaccg gtaacggctg
     3721 actgggggct ggcaggccgc gttcctcagc ggcctgctac acccctcgcc ccacgcgccg
     3781 ggcggggtgc tggatcgcga ggaacgcgtg ccaggcgtcg tggtagatgc gctcgcgggc
     3841 gtacccctgg cgctgcagga ccgcctcctt gaagttgtcg tagtcgatgt cgcggacggc
     3901 ccgcgagaag gcctcgacca cctgctctcg cggcgcctgc gcgcggaacc ggtagtcggc
     3961 cgattcgtcc tcggcgatcg cgcccagctc gggcaggtat ctctggcgga gcgtttccag
     4021 gtctttccga gcccgcgctc tcaccgtgag ggacccgcgt tcccggtccc acgccttctc
     4081 gaccacgctg tagaagccga acgtcgtcac gagccacatg cgcgcctccc gggccgtagg
     4141 cagggtgacc gagcggtcgt tcgtctcgct gcgcgccgct atcatagacc gacttcatct
     4201 gaagcgtggc ggagacagcc tggtgcaagt ggagggggca ccttgcgccc gcgcaggcgg
     4261 agaacggcaa ggtgcccttg actgggccga gggctactcg aggcggttgc gggcggcgat
     4321 cagcttttcc acgacctgga ggatggcggc ctcggtggcc tcgccgagga ccgtgtcacg
     4381 gaagttggac gagccgaact cgatgtgccc gctcacccgg ttggccccgc cgccgagcag
     4441 caggccgctg cggctcgagg tggcgtcgcc cttcgcgctg gccatgatct cgccggtcgt
     4501 cgtatcgatc atccgggcgg tgacggcgac actcgccttg cccttcttcg tcccgacgtc
     4561 gccgacgccg aagttgcggc gtccgaggac gctgccgcca aaccgggtgt tcttgtcttc
     4621 catcccgaac ttggtgaccg accccacgat gaggtacttc acccccaacg ccttgccgat
     4681 cttcacgacc tggccctggg agggatcggc ccggtcgctg ttcgagaagt cctgctcggc
     4741 cagcacgtcg ctgatgcgct tgcgctcgat gacacggtag gtcccgtcct cgacgaggcc
     4801 gtccacgatc atgtcggcga tccccttgcc aatgtcctgg ttgccgctcc accagtgctg
     4861 gatcgaggcg aagtcgaagt cgagcagcgc caccgtcggg cgattcgact gggcggcagc
     4921 cgggatggcg agcgtcagga gtgcgatcgt caggagcaac cacgaacccg gatggaacct
     4981 gcgcatgctt tcctcccgct gaaggtcacc gaaagtcgtt actagctcag ccgtggcagc
     5041 ttagccgggg ccctcggggg ccgtcaaggc ggccggcgcg gccggcaggg ccggcggcag
     5101 ggccgatggg gcctgacgac ttatcgtcgg gaacgggctg gccggatagg ccgtctcagc
     5161 gcctggccgc cggcttcctc gcctgccgct tcctggccag ttccgggggt cggcgagcat
     5221 cttgatgaac acgccgccga ccatgctgcg cgcctggagc ccggcgatgg gcgagggcgg
     5281 cgagcgccgc gtacttgggg ccgcccgcct gctcgagatc ccgcatgagt tcctcgtcat
     5341 accggcgggc gcgctcgacc atcgcgggcc cctcggagag ggctgtcctc atgagggcga
     5401 tggcgtcggc gccgttgcgg cgccagtagg gccgcagcgt cacgccttgc ccctttttcg
     5461 cgttgcccgg gcccgcgtgg gggcgataat cacctgggct gttccacaac aacaaagttc
     5521 gggcgggcga tcgcgtcgag gagcgtggcc gagtcctcga tggccgaacg ccccagctgc
     5581 cgtggcctgc ccacggccag gaggagtgtc atgagcgcga tgcagcagac ggcccggcgg
     5641 ttcttcgagg cgtgcgagac ggggaagggc tgggcggagt gccagcagta ctgcgtcccg
     5701 ggtgccacgt tcgcggcgca ggccgaggcc ctggccggcg tgagcacgct ggaggcgtac
     5761 acggaatgga tgaagggact cttcacgccg ctgccggacg ggcgctacga cctgcggtcg
     5821 tttgcggtgg acgaggagcg gcaccacgtg gtcgcgtacg gcgtctttca cggcacgcac
     5881 tcgggcgaag gcggaccggt tccgccggcg gggaagcagg tggcgtccga ctatgtctac
     5941 atcatggagt tcgatggcga ccggattcgc cacatgacaa aggtgtggaa cgacgcggcc
     6001 gcgctgaagc agttggcgtg gatgtgaggg gcgggcgagg cgccctcgtt accaggcgag
     6061 ggcgccgacg ggctggatcg gcgcgttgac gttcttccgc cactggcgga agttcttcgc
     6121 cgagttcagg tagccggtgc cgacggcgaa ggcggcgcgc tcgacgatcg aggcgatgcg
     6181 gatcagccgc ttgtgctggg gaaagcgggc gctcaaggcc cgcgtggcgg cgtggttgat
     6241 cgcgttgttg gcggcggagt agccgaggac gcgcagcgag acctcgaggt tgagccggtg
     6301 cccggtggcg cgggagacgg ggcggtcgtg aggcaggccg gagatcgtgt agtgcgggtt
     6361 ggcttcgacg tagccggcgg cgaagaagcg ctgcgaggaa acccagtcga gcgtcttgcc
     6421 gatttccgag gcggcggccg gggcgtaggt ggtcgggtct ttgaggacgt cgagagcgac
     6481 gccgcgggcg tgttcggcga tggactgggc gtgggcaggg gcggcggcga aggcggcgag
     6541 gaggacagca aggatggcgg ttctcatgtg cggtagcccc cgggccgcgc atcccatttg
     6601 gcggccctgc aacacctagc tcaagtgcaa aggtgtgccc gtcggcaaat atgcgaattt
     6661 cggcctgatt cccgcggatn nnnnnnnnna ccgtagactt tggtcgcgtt agtcagggaa
     6721 gtccgattgg aggcgaagat agtctaccgc agttcgtccg cgccggagac tgttcccgag
     6781 tcgcatgccg acccagcggt tcggcgatgc ggtgcaaaga ggcggcgctg gccgccggcg
     6841 acagttccgc gagggcctgg ccgaggcgag cggcgccgta gctcgcctat ctcacggtgg
     6901 tccgtgccgc tccctccgag gtcatcgaaa gaacggtccg tgccgtggct gcgcatagac
     6961 cgctcactca gcggcgttga cagacagagg gttgcgccgc agagttatgc gtgccgttgt
//
LOCUS       DAOPQX010000190         7000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11922_length_7000_cov_3.784737, whole genome shotgun sequence.
ACCESSION   DAOPQX010000190 DAOPQX010000000
VERSION     DAOPQX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11922_length_7000_cov_3.784737"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2042)
                     /locus_tag="PKK95_10740"
     CDS             complement(<1..2042)
                     /locus_tag="PKK95_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18738.1"
                     /translation="MSPTVSRPGLVRLALAAFTAWFMLVVGVYYRRAWALALEGPPAW
                     IPDGGWYELVPALGAWGLAISVASVVLTLLQRARPGLPVHSRPWRVTPLAALGALGAA
                     ALLAVAWPRPVAGRILPWLGEAVTRNLRGVAAAAVVVLAAYLAGALLLGLLKWRGGHW
                     ADRLLFRTAVGFGALSYLWLALCALGWYRPRAVVATLAAVLLGGAAWLARGTIRRRHA
                     SPHSAIKPSSPAPAGTVALAWIVVTAIAMVLALVPALAPEVEYDALWYHLQLPAVYLQ
                     HGTLVDVPHEFVSLYPMTWELLFGAGLVLGGAVGAKLLHFVCLPLVALAVWRFAGRFL
                     PRSSPWLAVAIAVTVPTVLWEATTAYIDLALALYTTLGLYSLLVYLESGDRRSLAVAI
                     ACFGIGAALKHLGLVAVAIVGLGLFVQLWRTTSFGRAALVTAALAAGSLLFAVPWHLR
                     SWLLSGNPFFPEMFGLFGASPERWDAAAERGLAGFKARLGFPRTPLNLLLLPWNMTMY
                     QARCAGALGPIFLAALPLMAWRARATAASGWLAYLVVSYVAIWASPISSYQIRFLVPI
                     VPALALLSSEGIGALLAPVPRNVRAVATAALVVLLLANLPPFIPLHEGNREGWDGWLT
                     HVVRGVPLRVVAGREPADEYLRSRVPTYGAWVHAARLPRTTRVLEFGGGDNYYATR"
     gene            complement(2044..2868)
                     /locus_tag="PKK95_10745"
     CDS             complement(2044..2868)
                     /locus_tag="PKK95_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18739.1"
                     /translation="MTTSRDPLDTAAYLVFALAVLFAAASVGNIVLPAAYARETASWA
                     AQGLGQDWVDVLVVVPVLLLSALLARRGSRRAALVMGGATAYTTYSLVLYAFAVHFNQ
                     LFLVYGVALGAGFYALVNLVVSYSRGDRRSWLSPGRLASRAAGAFAVFLGLAFYTLWL
                     AEIIPAIAAGRAPETLAEVGLITNPVQVLDVGIVLPAFVVGGVALLRGRRLGYWLVPV
                     MLTFAVLMDLALIGMDVSMATRNVPGGGQRILMFAVMAAVSLGLCWGMLRSGAREN"
     gene            3021..6671
                     /locus_tag="PKK95_10750"
     CDS             3021..6671
                     /locus_tag="PKK95_10750"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOC18740.1"
                     /translation="MNLAGPRRTLAVVLFVVGWATHAQAALVVRNLHVEYRTTPLGID
                     VREPRFGWQVHATSGERGVTQAAYRLEVRGAGGALVWDSKRVGSADSLGVTYGGAPLQ
                     ASTRYTWTVTVWTGAGEELTANSWFETGLMDSKPGAPAWGGAEWIGGGDEDLVLYSPY
                     LAIFHVSYLVAIAPGSTSASFVYGANDSRLMDRYKNVSQLENARDHSYIKLELDVSRV
                     DGSRDGKARLHVYRAGYAPADDPARPFKTFEIDTALVNQANKHAEHAVAFRSAFGQIW
                     LSVDGSAAFAGAPAPATQNTAGWTGRNTAPNAVNLNPMGQGGDYLPFGLLCDIGFSAG
                     PGQGATFREVIVRNDRAPANVLFREELAGPVYDGIFADAPARSGFSVSGGKYVLSGAD
                     EGVFLVRDPSRNSMPMLRTTFTTPRKAVASARLYVTSRGIYEMFLNGQRVGDDYYNPG
                     LTQYNVTHLYQTYDVTRLIVPGNNAMGAMLGEGWWSGLLSFGSIRNHFGDRQSLLARL
                     VVTYQDGTSDTVTTNDRTWKYYGRGPLVYGSLDFGEVYDASREAVVSGWTSAGYDDYA
                     WKPAVVVPLEGTTFSGVEAGRGGVPGTSFSFDRLSLVGQIGETAGVFRVLTARSVKEV
                     RPGVFVYDLGQNLVGVPRITIDDGRAGAKLTLRYSEMLYPDLQESGKNVGMIMTENYR
                     AALSQDVYTMKAGRQVFQPRFTSHGFQYVEITGIDRALPLSAVEGVAISSIRSLTAEY
                     RTSSEKVNRLWSNLVWSNAGNFLTIPTDCPQRNERMGWSGDINVFSRTATYVSNADQF
                     LTRHMQAMRDVQAPNGRFTDIAPVGGGFGGVLWGSAGIVVPWETYLQYKDLRLLETHY
                     PAMAAYVDYLETTIDPKTGLSRDAQLGDWLGPQNNALGTAFLVTAYHAYDLGIMARVA
                     ELLGKPGDAARYRSLYEKRKVFFNGTFVNAEKTLATGGAFGGFGGGVGPAKPPEFRVA
                     DTQTSYAVGLGMHLFDAAIVPDMIANLADAVARENKDDDGVVRPKYSLMTGFIGTAWI
                     SKALSANGLSELAYRLLLNDRYPSWLYAIDQGATTIWERLNGYTVENGFGGNNSMNSF
                     NHYSFGAVGQWMMAHSLGIERGEPGFGEFVLQPEPDPTGTMTFAEGYYDSMPGRIVSA
                     WKVAGPVLTYRATVPPNTAATLRLPTSSADSVREGGVSAQSARGVAFVKYEDGKAVFR
                     LESGTYEFTASR"
     gene            6678..>7000
                     /locus_tag="PKK95_10755"
     CDS             6678..>7000
                     /locus_tag="PKK95_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOC18741.1"
                     /translation="MLTYVLFGISYGFAAAAQPGQLQAYLISRAVSHGWRRTLPAVFA
                     PLLSDLPVIIVVLLILTRVPPAMVSALRLAGGVFLLYLAGEAIRRWRAREGGDLHPDV
                     PAHRT"
BASE COUNT         1181 a         2374 c         2520 g          925 t
ORIGIN      
        1 tccctggtgg cgtagtagtt gtcgcccccg ccgaactcca gcaccctcgt ggtgcgcggc
       61 agccgcgccg catgcaccca agccccgtag gtcggcaccc gcgaccggag gtactcgtct
      121 gcgggctcgc gccccgcgac gacgcggagc ggcaccccgc gaaccacgtg cgtgagccag
      181 ccatcccacc cttcgcggtt gccttcgtgc agcgggatga acggggggag gttggccagc
      241 agcagcacga ccagggccgc cgtggcgacg gcgcggacgt tcctcggaac cggcgcgagc
      301 agggcgccga tcccctcgga ggacagcagc gccagcgcgg gaacgatcgg caccaggaag
      361 cggatctggt agctcgagat tggcgaggcc cagatcgcaa cgtaggacac gacgaggtac
      421 gcgagccacc ccgacgccgc ggtggcgcgc gcccgccagg ccatcagcgg cagcgcggcc
      481 aggaagattg ggcccagagc gcccgcgcac cgcgcctggt acatcgtcat gttccacgga
      541 aggagcagca ggttcagcgg tgtccgcggg aatcccaggc gggccttgaa ccccgcaagg
      601 ccccgctcgg cagccgcgtc ccatcgttcg ggcgacgcgc cgaacaggcc gaacatttcc
      661 ggaaagaacg ggtttcccga gaggagccag ctccggagat gccacgggac cgcgaagagc
      721 aggctgcccg ccgcgagggc cgcggtgacc agcgcggcgc gcccgaacga cgtcgtgcgc
      781 cacagctgaa caaacagccc gagcccgacg atggccacgg cgacgaggcc gaggtgcttg
      841 agggcggcgc cgattccgaa acaggcaatc gcgacggcga gcgatcgccg gtcgcccgac
      901 tcgaggtaga ccagcagcga gtacaggccg agcgtcgtgt agagcgccag cgcgaggtcg
      961 atgtatgccg tggtcgcctc ccacagcacg gtcggcacgg tgacggcaat tgcgactgcg
     1021 agccacggcg acgagcgggg gaggaagcgc cccgcgaacc tccagacggc gagcgcgacg
     1081 agcggcaggc agacgaagtg gagcagcttg gcgccgaccg cgcccccgag cacgagtcct
     1141 gccccgaaca gcagttccca cgtcatcggg tagagggaga cgaactcgtg gggaacgtcc
     1201 accagtgttc cgtgctgcag gtacaccgcg ggaagctgca ggtggtacca gagcgcgtcg
     1261 tactccacct cgggcgcgag cgccggcacc agggccagga ccatcgcgat cgccgtcacg
     1321 acgatccacg cgagcgcgac cgtgccggct ggcgctggtg aagagggctt gatcgcggaa
     1381 tgcggcgagg catgccgtcg ccggattgtg ccgcgcgcca gccaggcggc gccgccgagc
     1441 aggacggccg ccagcgtggc gacgaccgcg cgtggacggt accagccgag cgcgcagagg
     1501 gcgagccaca ggtacgacag cgcgccgaat ccaacggcgg tgcgaaacag caaccggtcg
     1561 gcccagtggc ctccgcgcca cttcagcagg ccgagcagca gggcgcccgc gaggtaggcg
     1621 gccaggacga cgacggcagc ggcggcgacg ccccggaggt tccgggtcac agcctcgcca
     1681 agccacggga ggatccggcc ggcaacggga cggggccatg cgactgccaa cagggcggcg
     1741 gcgcccagcg ctcccagcgc cgcaagcggc gtcacgcgcc aaggtcgcga gtggaccggc
     1801 agcccgggtc tcgcccgctg cagcagcgtc agcacgacgg aggcgacgct gatcgcgagg
     1861 ccccaggcgc cgagcgccgg aaccagctcg taccacccgc cgtccgggat ccaggccggg
     1921 ggcccttcga gcgccagcgc ccacgcccgg cggtagtaga ctccgacgac gagcatgaac
     1981 caggcggtga aagccgccag cgcgagacgg acgaggcccg gccggctgac ggttggtgac
     2041 acctcagttc tcccgggcgc cgctcctcag catcccccag cagagcccca gcgacactgc
     2101 cgccatcacc gcgaacatca ggattcgctg gccgcctccc ggtacgttcc gcgtggccat
     2161 cgacacgtcc atcccgatga gcgcgaggtc catcagcacg gcgaaggtga gcatgacggg
     2221 taccagccag tagcccagcc tcctgccgcg cagcaaggcc accccgccga ccacgaaggc
     2281 gggcagcacg atgccgacgt cgagcacctg cactgggttc gtgatgaggc cgacttcggc
     2341 gagggtctct ggcgcccggc cggcggcgat ggccgggatg atctcggcga gccagagcgt
     2401 gtagaacgcc aggccgagga acacggcgaa ggcgcccgcg gcgcggctgg ccaggcggcc
     2461 cggcgacagc caggacctcc ggtcgccgcg gctatacgag acgacgaggt tcacgagcgc
     2521 gtagaacccg gcgccgagcg cgacgccgta gacgagaaac agctggttga agtgaacggc
     2581 gaacgcgtag aggacgagcg agtacgtggt gtaggcggtt gccccgccca tgacgagtgc
     2641 cgcgcggcgc gacccgcggc gggcgagcag cgccgaaagg agcagcacgg gcacgaccac
     2701 gagaacgtcc acccagtcct ggccgaggcc ctgcgcggcc catgaggccg tctcgcgcgc
     2761 gtacgcggcc ggcagcacga tgttgcccac cgaggcggcg gcgaagagaa cggccagcgc
     2821 gaacaccagg taagcagccg tgtcgagggg gtcgcgcgag gtcgtcatgc cgggcgaatt
     2881 gtaccatcgg ccgccggtgc agcctgtgcg cgtgcgggcg tcgagcgttc cagcaggctc
     2941 gggctggtga tacgctacct cggcctcgcg gctggccgaa gggccaggtg ggccgctcgt
     3001 gggcaagaga gggggacacg gtgaatctgg ccgggccgag acgcacgctt gcggtggtgc
     3061 tcttcgtggt gggttgggcg acccacgcgc aggcagccct ggtcgtccgg aaccttcacg
     3121 tcgagtacag gaccacgccg ctcgggatcg acgtccgcga gccgcggttc gggtggcagg
     3181 tgcacgccac ctcgggcgag cgcggcgtca cgcaggcggc gtaccggctc gaggtgcgcg
     3241 gagcgggcgg cgccctcgtc tgggactcga agagggttgg gagcgcggac tcgctcggcg
     3301 tcacctacgg cggcgcgccc ctgcaggcct cgacccgtta cacctggacc gtgaccgtgt
     3361 ggaccggtgc gggggaggag ctgaccgcca actcctggtt cgaaacgggg ctgatggaca
     3421 gcaagcccgg cgctcccgcg tggggcgggg cggaatggat tgggggcggc gacgaggacc
     3481 tggtgctcta ctcgccctac ctggcaatct tccacgtgag ctacctggtg gccatcgcgc
     3541 cgggcagcac gtccgcgagc ttcgtgtacg gggcgaacga ctcgcgcctg atggaccggt
     3601 acaagaacgt ctcgcaactc gagaacgccc gcgaccacag ctacatcaag ctcgagctcg
     3661 acgtctcgcg ggtggacggc tcgcgcgacg gcaaggcgag gcttcacgtc tatcgcgctg
     3721 gctatgcccc cgccgacgat ccggcgcggc ccttcaagac cttcgagatc gacacggccc
     3781 tcgtcaacca ggcgaacaag cacgccgagc acgcggtcgc gttccgcagc gccttcggcc
     3841 agatctggct gagcgtggac ggcagcgcgg cgtttgccgg cgcccccgcg ccggcgaccc
     3901 agaatacggc gggatggacg gggcgcaaca ccgcgccgaa tgccgtgaac ctgaacccga
     3961 tggggcaggg cggcgactac ctgccgttcg gcctgctgtg cgacatcggg ttctcggcgg
     4021 gcccgggaca gggcgccacc ttccgcgagg tgatcgttcg caacgatcgg gcgccggcga
     4081 acgtcctgtt ccgcgaggag ctggccgggc ctgtctacga cggcatcttc gcggacgctc
     4141 cggcccggtc gggcttctcg gtctccggcg ggaagtacgt gctgtctggt gcggacgagg
     4201 gtgtcttcct ggtgcgcgac ccgagccgga actcgatgcc gatgctcagg accaccttca
     4261 cgacgccgcg caaggcggtc gcgtcggcgc ggctctacgt cacctcgcgc ggcatctacg
     4321 agatgttcct gaacggtcag cgcgtgggcg acgactacta caaccccggc ctcacgcagt
     4381 acaacgtcac ccacctgtac cagacctacg acgtgaccag gctgatcgtg ccgggcaaca
     4441 acgcgatggg cgcgatgctg ggagaggggt ggtggagcgg gctcctcagc ttcggcagca
     4501 tccggaacca cttcggggac cggcagtcgc tgctcgccag gctggtggtc acctaccagg
     4561 acggcacgtc cgacaccgtc acgaccaacg atcgcacctg gaagtactac gggcgcggtc
     4621 ccctcgtcta cggcagcctc gacttcggcg aggtgtacga cgcctcccgc gaggcggtgg
     4681 tatcggggtg gacgagtgcc ggctacgacg actacgcgtg gaagcccgcg gtggtcgtcc
     4741 cgctcgaggg aaccaccttc tcgggagtcg aagccgggcg cggcggcgtg ccgggaacca
     4801 gcttcagctt cgaccggctg tcgctcgtgg ggcagatcgg ggagacggcc ggcgtgttcc
     4861 gcgtgctgac cgccaggagc gtgaaggagg tcaggcccgg ggtgttcgtc tacgacctgg
     4921 ggcagaacct cgtcggcgtg ccgaggatca cgatcgacga cggccgtgcg ggagcgaagc
     4981 tgacgcttcg ctattccgag atgctctacc ccgacctgca ggagtcaggc aagaacgtcg
     5041 ggatgatcat gaccgagaac taccgggcgg cgctcagcca ggacgtgtac acgatgaagg
     5101 cgggccgcca ggtgttccag ccgcgcttca cgtcgcacgg cttccagtac gtcgagatca
     5161 cgggcatcga tcgcgcgctt ccgctctcgg ccgtcgaggg ggtggcgatc agctcgatcc
     5221 gcagcctcac cgccgagtac cggacgtcga gcgagaaggt gaaccggctg tggtcgaacc
     5281 tggtctggtc gaacgcgggc aacttcctca cgatcccgac cgactgcccg cagcgcaacg
     5341 agcgcatggg gtggtcgggc gacatcaacg tgttctcgcg gaccgcgacc tacgtctcga
     5401 acgccgatca gttcctcacg aggcacatgc aggcgatgcg cgacgtccag gcaccgaacg
     5461 ggcggttcac cgacatcgcc ccggttggcg gcggcttcgg cggcgtcctc tggggcagcg
     5521 ccggcatcgt cgttccctgg gagacgtacc tccagtacaa ggacctgcgc ctgctcgaga
     5581 cgcactaccc ggcgatggcg gcgtacgtcg actacctcga gacaacgatc gatccgaaga
     5641 ccggcctgag ccgggacgcg cagctgggcg actggctggg gccgcagaac aacgcgcttg
     5701 gaacggcgtt cctcgtcacc gcctaccacg cctacgatct cgggatcatg gccagggtgg
     5761 cggaactcct gggcaagccg ggcgacgcgg ccaggtaccg cagcctgtac gagaagcgga
     5821 aggtgttctt caacggcacc ttcgtcaacg cggagaagac gctggccact ggcggcgcgt
     5881 tcggcgggtt cggcggcggg gtcggccctg cgaagccgcc cgagttccgg gtggccgaca
     5941 cccagacctc gtacgcggtg ggtctcggca tgcacctctt cgacgctgcc atcgtgccgg
     6001 acatgatcgc gaacctcgcc gatgccgtgg cgcgcgagaa caaggacgac gacggggtgg
     6061 tgcggccgaa gtactcgctg atgaccgggt tcatcggcac cgcctggatc agcaaggcgc
     6121 tctccgcgaa cggcctgagc gagctggcct accggctgct cctgaacgac cggtacccgt
     6181 cgtggctcta cgcgatcgac cagggcgcga cgacgatctg ggagcgtctc aacgggtaca
     6241 ccgtcgagaa cgggttcggc gggaacaaca gcatgaactc gttcaaccac tactccttcg
     6301 gggccgtggg gcagtggatg atggcccact cgctgggcat cgagcgcggc gagcccggct
     6361 tcggagagtt cgtcctgcag cccgagcccg acccgaccgg caccatgacg tttgccgagg
     6421 gctactacga ctcgatgccc ggccggatcg tcagcgcctg gaaggtggcc gggccggttc
     6481 tgacctaccg ggccaccgtg cctccgaaca ctgcggccac gcttcgcctg cccacttcgt
     6541 cggccgactc ggtgagggag ggtggggtca gcgcgcaaag tgcgaggggg gtggccttcg
     6601 taaagtacga ggacgggaag gcagtcttca ggctggagtc gggaacctac gagttcacgg
     6661 cgagccgctg agcggcgatg ttgacctacg tgctcttcgg catcagttac gggttcgcgg
     6721 cggcagccca gcccgggcag ctgcaggcgt atctcatctc gcgcgcggtg tcgcacggct
     6781 ggcggcgcac gctgcccgcc gtcttcgcgc cgctcctcag cgacctcccg gtgatcatcg
     6841 tcgtgctgct gatcctgacg cgcgtgccgc cggcgatggt gagcgcgctg cggttggccg
     6901 gcggcgtctt ccttctctac ctggccgggg aggccatccg gcgctggcgg gcgcgcgagg
     6961 ggggcgatct ccatcccgac gtgccggcgc accggacgtt
//
LOCUS       DAOPQX010000191         6978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_11956_length_6978_cov_3.960277, whole genome shotgun sequence.
ACCESSION   DAOPQX010000191 DAOPQX010000000
VERSION     DAOPQX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11956_length_6978_cov_3.960277"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKK95_10760"
     CDS             complement(<1..679)
                     /locus_tag="PKK95_10760"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOC18742.1"
                     /translation="MARIGVTALVLLLLAAATPSRAQESLPELTRPVNDFAGVVDAAS
                     ADAMDRVIRSLQAATGDAVVVATVRSVEPYGDIREYAVRLFENAGRGIGEKGKDNGLL
                     VLLATEDRRVWIEVGYGLEPFITDGFAGETSRQYMVPAFRRGEYGAGLLAGVERLVGR
                     IADGRGVTLEGVRPVRAERRAPGGVRPGVIIGLIILFLILRMISGGGGRGRRIGPERG
                     GAPVPATV"
     gene            complement(691..1392)
                     /locus_tag="PKK95_10765"
     CDS             complement(691..1392)
                     /locus_tag="PKK95_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18743.1"
                     /translation="MGKRLNRDEAVSRLRGDLSQVLGERLHSLVVYEAAAGDDHVVHA
                     LAVIEAFTPDDLARLAPAAAGWAAAGLAVPLVFGRAELVRSLDAFPLEFSQILARHEV
                     VAGEPLLEGLSVRREDLRRACEAQARSHLVHLREGYLEAGGSPKEVAALVEGSLASLR
                     ALLVSVARLHGANPRTPDEIAAFLAEGLPASAAGLRPLVTHPAGQPLPLSEAEHVFPG
                     YVVAIEQLARTLDRL"
     gene            1551..2519
                     /locus_tag="PKK95_10770"
     CDS             1551..2519
                     /locus_tag="PKK95_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOC18744.1"
                     /translation="MQLRELKVGCFGGGTGLPSVLGGLKTNPWLHVNAIVTMFDSGGS
                     SGVLRDQLGVLPPGDILKCTLALARNESEARRVLLSRLPTLEQHERLAGHTGGNLLLS
                     MMERHSGDFLAAVDGLRALLGCSGRVWPASVEQASVCARYGDGTTTSGEVEVDELQIE
                     GRLVREVWLEPVPHIHPAVAQAIPGFDAAIIGPGSFFTSLLPPLMVAGMREALAEMKG
                     PILLVANLLTEGRGMNGFTAGDAVRWISRVIGRDVDVLLVNTGRPEPETLARYAAEHK
                     HPLPLGDVPSCCEVVEGEFWDGEIARHSRRRLSYAIWGVLARRLLR"
     gene            2594..3373
                     /locus_tag="PKK95_10775"
     CDS             2594..3373
                     /locus_tag="PKK95_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HOC18745.1"
                     /translation="MAKRPLAEYRAKRDFSKSPEPSGEAKPSRKKARRRFFCVQKHLA
                     SHLHYDFRLEHDGVLLSWAVPKGPSLDPAEKRLAMHVEDHPLDYGEFEGVIPEGYGAG
                     IVMLWDKGTWEPEDADVGAALERGDLKMRLDGYKLKGSWVLVRTRRDEGRSWLLIKHR
                     DEWSSPELDILEFAPLSVKSEGDFADILAQDRPDIWISNRPARGGEAGALFQKIVERA
                     LAIKQAREGKGARPVDSARTRLAQGAAGKKTTAAKAGTKRR"
     gene            complement(3525..5348)
                     /locus_tag="PKK95_10780"
     CDS             complement(3525..5348)
                     /locus_tag="PKK95_10780"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOC18746.1"
                     /translation="MEMAGGPLHVTDLTFRDGHQSLFATRVRTADLAALAPAWDGIGF
                     WSMEVWGGATFDVMTRFLNEDPWERIRLLKQRLPHVRLQMLLRGQNLVGYRNYADDVV
                     RAFVAHAAECGIDVFRVFDALNDERNFVTAFEAIKAAGRHVQGALSYSITERSLGGPV
                     YTVEYYLAKARAIERMGADSLCIKDMAGLLSPGDADTLVRALKREIAIPLVVHTHFTS
                     GMAYMTLLKAIEAGADGIDACVAPFALRTSHAAVEPIVAALAGTARDTGLDLDALVRA
                     GDDLERIAPKYREFLDDTKLSIIDPGVLSHQIPGGMFSNMVAQLRQAGALDRLPEVYE
                     ELPRTRRELGFPPLVTPTSQIVGTQAVLNVLFGRYKMIAREVRDYVFGLYGKPPAVID
                     ADVRRLALAGYERGEEPITVRAADLLDPEMEKAAEATRGLARDVGDVLCCALYPTTGL
                     RFLKWKYGVEAPPPETRPRTLEDVAREQALVERAKKGELSAAPSTSGPRRFSVRVGED
                     LFSVEVEEGPATERGAVSAQGRPTVPSLDASVVTAPMPGLLVRYLVRPGDVVKRGQGV
                     VVVEAMKMQNTLPAARDGRVASLDAREGDNVGKNQVLLTLE"
     gene            5389..6645
                     /locus_tag="PKK95_10785"
     CDS             5389..6645
                     /locus_tag="PKK95_10785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC18747.1"
                     /translation="MTTPLFSWLREAPAASKRALLAACLGWALDSFDVNLYALVLASL
                     LADLEMTKATAGLIGSLTLVSAAAGGIAFGLIADRYGRTRALMGSILIYSLFTGACGL
                     AQTWWHLLVFRVLLGFGMGGEWASGAALVSETWPAAHRGKALGLVQSSWAIGFAAAAL
                     MTHVVLPAWGWRAVFFVGVLPALATAWVRRSVEEPEIWRHQREARAREGVIAPEAVRP
                     GLASIFRRPLLRLTVFVTLMNACTLFAYWGFTQWVPAYLELSPESGGLGLAGQKTTLI
                     VAMNVGMWFGYVTFGLVSDALGRKRIYVAYVLAAACFVMAYAMVRAPVALLLLGPFVG
                     FFGAGYFSGFGAVTAEIYPTRIRATAQGFTYNLGRVASALAPFTVGSLAQSRGFTAAF
                     ATVALAFVLAAVAWIWIPETKGRELA"
     gene            6780..>6978
                     /locus_tag="PKK95_10790"
     CDS             6780..>6978
                     /locus_tag="PKK95_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18748.1"
                     /translation="MKRLFLFSCLLGLLASPVASSSPAAVPQEPVSTRSATPAPAPRP
                     SLVVVLVVDQMRADYIDQYGDT"
BASE COUNT          959 a         2480 c         2495 g         1044 t
ORIGIN      
        1 gaactgtcgc cggaacagga gcgcctcctc gctcagggcc gattcggcgt ccgcgtccgc
       61 cgccgcccga gatcatccgg aggatcagga acaggatgat caggccgatg atgacaccgg
      121 gccggacccc gccgggggcg cgccgttcgg ctcgcaccgg gcgcacaccc tcgagcgtca
      181 cgccccggcc gtcggcgatc cgtcccacca ggcgttcgac gcctgcaagc aggccggcgc
      241 cgtactcgcc gcggcggaac gcgggcacca tgtactggcg gctggtctct cccgcgaaac
      301 catcggtgat gaacggctcg agcccgtagc ccacctcgat ccagacgcgc cggtcctcgg
      361 tggccagcag gacgagcagg ccgttgtcct ttcccttctc gccgatgccg cgaccggcgt
      421 tctcgaagag gcggaccgcg tactcccgaa tgtcgccgta gggctcgacg ctgcgaacgg
      481 tcgccaccac gaccgcgtcg ccggtggccg cctgcagcga gcggatcacc cggtccatcg
      541 cgtcggcgct ggccgcgtcg acgactcccg cgaagtcgtt caccggccgg gtcagttcgg
      601 gcagcgattc ctgggcacgc gacggcgtag ctgccgcgag caggagcagg acgagggccg
      661 tgacaccgat ccgcgccatg cagcgggaca tcagagccga tccagtgttc gagcgagctg
      721 ctcgatggcc acgacgtagc ccgggaagac gtgctccgcc tcggacagcg ggagcggctg
      781 gccggccggg tgcgtgacga gcgggcgaag ccccgccgcc gacgccggca ggccctcggc
      841 gaggaaggcg gcaatctcgt ccggagtgcg cgggttggca ccgtgcaggc gcgccactga
      901 aacgaggagc gctctcagcg acgcgagcga tccttccacg agcgccgcca cctccttggg
      961 tgaaccaccc gcctcgaggt agccctcgcg caggtggacg aggtggcttc gcgcctgggc
     1021 ttcgcaggcg cggcggaggt cctcgcggcg aacgctcaag ccctcgagca gtggctcgcc
     1081 ggcgaccacc tcgtggcggg cgaggatctg gctgaactcc aacgggaagg cgtcgagcga
     1141 gcggaccagt tcggcccggc cgaacacgag cggcacggcc agccccgccg cggcccaccc
     1201 ggccgctgcc ggcgccagcc gggcgagatc gtccggcgtg aacgcctcga tgacggcgag
     1261 ggcgtgaacc acgtggtcgt cgccggcggc cgcctcgtag acgaccagcg aatgcaggcg
     1321 ctcgcccagc acctgcgaca ggtcgccccg cagccggctg accgcctcgt cccgattgag
     1381 ccgcttgccc atgttcctag tctacccgcc ctccgccctc cgctcccgcc ttccgcctcc
     1441 cgtccttgcc gcggccatca ctccctcggc ggcaccctga gcaaggcgcg tgcgcgccgg
     1501 gtcgaagggc cgtcctccgc ctgacgtccg tcctgtaaaa tgggggcgcc atgcaactgc
     1561 gcgagctgaa ggtcggctgc ttcggcggcg gcacggggct gccgagcgtc ctcggcggct
     1621 tgaagacgaa cccctggctt cacgtcaacg cgatcgtcac catgttcgac agcggcggca
     1681 gctcgggcgt gctccgcgac cagcttggcg tgctgccgcc gggcgacatc ctgaagtgca
     1741 cgctggcgct cgcgcgcaac gagtcggagg cgcggcgggt gctgctgtcg cggctgccga
     1801 cgctcgagca gcacgagcgg ctcgcggggc acaccggtgg caacctcctg ctctcgatga
     1861 tggagcgcca cagcggcgac ttcctggccg cggtggacgg cctgcgcgcg ctgctgggct
     1921 gcagcggccg cgtctggccc gcgagtgtcg agcaggcctc ggtctgcgcg cggtacggcg
     1981 acgggacgac gacgagcggg gaggtggagg tcgacgagct gcagatcgag gggcggctgg
     2041 tgcgcgaggt gtggctcgag ccggtcccgc acatccaccc cgcggtcgcg caggcgattc
     2101 ccgggttcga cgccgccatc atcggcccgg gcagcttctt cacgagcctg ctgccgcccc
     2161 tgatggtggc ggggatgcgc gaggcgctgg ccgagatgaa gggcccgatc ctgctggtcg
     2221 cgaacctgct gaccgagggg cgcgggatga acggctttac ggccggtgac gcggtgcggt
     2281 ggatcagccg ggtcatcggg cgcgacgtgg acgtgctgct ggtgaacacg ggccggcccg
     2341 agccggagac cctggcgcgc tacgccgccg aacacaagca cccgctcccg cttggcgatg
     2401 tgccctcgtg ctgcgaggtg gtcgaggggg agttctggga cggcgagatc gcccgccact
     2461 cgcgccgccg gctctcctac gcgatctggg gcgtgctggc ccgccgcctg ctgcgctgag
     2521 ctgcggcaga gggtcggtga gcgggtgacc ccgaacacca cgtgctcggc ggggttgcgg
     2581 taagctgcag gtgatggcca agcgccccct tgccgagtac cgcgccaagc gcgacttctc
     2641 gaaatcaccc gagccgtccg gcgaggcgaa gccttcgagg aagaaggccc gccgccgctt
     2701 cttctgcgtc cagaagcacc tcgccagcca cctgcactac gacttccgcc tcgagcacga
     2761 cggcgtgctg ctctcgtggg ccgtgccgaa ggggccgtcg ctcgatccgg ccgagaagcg
     2821 gctggcgatg cacgtcgagg atcatccgct cgactacggc gagttcgagg gggtcattcc
     2881 cgaggggtac ggcgccggca tcgtgatgct gtgggacaag ggcacctggg agccggagga
     2941 cgccgacgtg ggtgccgccc tcgagcgtgg cgatctcaag atgcgcctgg acggctacaa
     3001 gctgaaggga tcgtgggtgc tcgtgcggac gcggcgcgac gagggccgga gctggctgct
     3061 gatcaagcat cgcgacgagt ggtcgtcacc cgagctcgac atcctcgagt tcgctcccct
     3121 cagcgtgaag agcgagggcg acttcgccga catcctcgcg caggaccgcc ccgacatctg
     3181 gatctcgaac cgccccgccc gcggcggcga ggcgggggcc ttgttccaga agatcgtcga
     3241 gcgcgcgctg gcgatcaagc aggcccggga gggaaaggga gcgcggcccg tcgactcggc
     3301 acgcacgcgc ctcgctcagg gtgccgcggg gaaaaagacg accgcggcaa aggcggggac
     3361 gaaacggcgc tgaaggcacg gggcggtccg cgtctcccaa gggcggcgcc agccggctcc
     3421 acgactcggt cgccgacgcc gacttcgtgg acctgacttc gcgacctccc gacttcgtgg
     3481 acttcgcgac ttcgtggact ggcgaaacgc cgtgtccgct gtcctcactc cagcgtgagc
     3541 agcacctggt tcttgccgac gttgtcgccc tcgcgggcgt cgagcgaggc gacgcggccg
     3601 tcgcgcgccg ccggcagggt gttctgcatc ttcatcgcct cgacgacgac aactccctgc
     3661 ccgcgcttca cgacgtcgcc ggggcgcacc aggtagcgca cgaggaggcc gggcatcggc
     3721 gccgtcacga ccgaggcgtc gagactcggg accgtgggcc gtccctgggc ggacaccgct
     3781 ccccgctcgg tcgccggtcc ttcctccacc tccacgctga agagatcctc gccaacccgt
     3841 acgctgaacc gccgcggccc ggacgtggac ggcgccgcgg acagctcgcc cttcttcgcc
     3901 cgctcgacga gcgcctgttc gcgcgccacg tcctcgagcg tgcgcgggcg ggtctcgggc
     3961 ggcggcgcct cgacaccgta cttccacttg aggaacctca ggcccgtggt ggggtagagc
     4021 gcgcagcaga ggacgtcgcc gacgtctcgc gccagcccgc gcgtggcctc ggccgccttc
     4081 tccatctccg ggtcgagcag gtcggccgcc cgaaccgtga tcggctcttc gccgcgctcg
     4141 tacccggcca gcgccagccg ccggacgtcg gcgtcgatca ccgccggcgg cttcccgtag
     4201 agaccgaaga cgtagtctct gacctcgcgg gcgatcatct tgtaacggcc gaacaacacg
     4261 ttcagcaccg cctgcgtgcc cacgatctgg ctggtcggcg tcaccagcgg cgggaagccc
     4321 agctcgcgcc gcgtgcgagg cagttcctcg tacacctccg gcaggcggtc gagggcgccg
     4381 gcctgccgca gctgggccac catgttcgag aacatccctc ccgggatctg gtgcgagagc
     4441 acgcccgggt cgatgatcga cagcttcgtg tcgtcgagga actcccggta cttcggcgcg
     4501 atccgctcga ggtcgtcgcc ggcccgcacg agggcgtcga ggtcgaggcc ggtgtcgcgg
     4561 gcggtgcccg ccagcgcggc gacgatcggc tcgacggcgg cgtgcgaggt gcgcagggcg
     4621 aacggcgcca cgcaggcgtc gatgccgtcg gcgccggcct cgatggcctt caggagcgtc
     4681 atgtaggcca tgccgctcgt gaagtgcgtg tgcacgacga gcggaatcgc gatctcccgc
     4741 ttgagcgccc ggacgagcgt gtcggcgtcg ccgggcgaga gcagcccggc catgtccttg
     4801 atgcagaggc tgtcggcccc catccgctcg atggcgcggg ccttggccag gtagtactcg
     4861 accgtgtaga ccggccctcc gagcgaccgc tccgtgatcg agtagctgag cgcgccctgg
     4921 acgtgcctgc cggccgcctt gatggcctcg aaggcggtga cgaagttccg ctcgtcgttg
     4981 agggcgtcga agacgcggaa cacgtcgatc ccgcactcgg ccgcgtgcgc cacgaacgcc
     5041 cgcaccacgt cgtcggcata gttccggtag cccacgaggt tctggccgcg cagcagcatc
     5101 tgcaggcgga cgtgcggcag gcgctgcttc aggaggcgga tgcgctccca cgggtcctcg
     5161 ttgaggaagc gcgtcatcac gtcgaaggtg gcgccgcccc agacctccat cgaccagaag
     5221 ccgatcccgt cccaggccgg cgccagggcg gccaggtcgg cggtccgcac gcgggtggcg
     5281 aagagcgact ggtgcccgtc gcggaaggtg aggtcggtga cgtgcagcgg cccgccggcc
     5341 atttccatga ggcctccgtc aaatgctcgg gatactatag ccgacggctt gaccacccca
     5401 ctcttctcgt ggctgcgtga ggccccggcg gcgtcgaagc gggcgctgct ggcggcgtgc
     5461 ctcgggtggg cgctcgactc gttcgacgtc aacctgtacg cgctggtgct cgcctcgctg
     5521 ctcgccgatc ttgagatgac caaggcgacg gccgggctga tcgggtcgct gacgctggtg
     5581 tcggcggccg ccggcggcat cgccttcggc ctgatcgccg accgctacgg ccgcacgcgg
     5641 gcgctgatgg ggagcatcct catctattcg ctgttcaccg gcgcgtgcgg cctggcccag
     5701 acctggtggc accttctggt gttccgcgtg ctcctcggtt tcggcatggg aggcgagtgg
     5761 gcgagcgggg ccgcgctcgt ctcggagacg tggccggcgg cgcaccgcgg aaaggcgctg
     5821 gggctcgtcc agagctcttg ggcgatcggt ttcgcggcag ccgcgctgat gacccacgtc
     5881 gtgctgccgg cctgggggtg gcgggcggtg ttcttcgtcg gcgtgctgcc cgcgctggcg
     5941 accgcctggg tgcggcgcag cgtcgaggag ccggagatct ggcggcacca gcgcgaggcg
     6001 cgcgcccggg aaggcgtcat cgcgccagag gccgttcgtc ccgggctcgc aagcatcttc
     6061 aggcggccgc tgctgcgcct cacggtgttc gtcacgctga tgaacgcgtg cacgctcttc
     6121 gcctactggg gcttcacgca gtgggtgccg gcctacctcg aactgtcgcc ggagagcggc
     6181 ggcctcggtc tcgccgggca gaagaccacg ctcatcgtcg ccatgaacgt cgggatgtgg
     6241 ttcggctacg tgaccttcgg cctggtcagc gacgcgctgg gccggaagcg gatctacgtc
     6301 gcctacgtgc tggcggctgc ctgcttcgtc atggcctacg ccatggtccg cgcaccggtc
     6361 gcgctgctgc tgctcgggcc gttcgttgga ttcttcggcg ccggctactt cagcggcttc
     6421 ggcgcggtga ccgccgagat ctacccgacc cggatccgcg ccaccgcgca aggcttcacc
     6481 tacaacctgg gtcgcgtcgc cagcgccctg gcgcccttca cggtgggctc gctcgcgcag
     6541 tcgcgtggct tcaccgccgc gttcgcgacc gtcgcgctcg ccttcgtgct cgccgccgtc
     6601 gcctggatct ggatccccga gaccaagggc cgcgagctgg cgtaggcggc ggggcctgcc
     6661 gggccccgcg aagtcccgcg agccacggcc caagcgagcc cgcccagcgc cagaggaggg
     6721 gaggagggga ggcggacggc cttcggcccc cagcgggctt tgtgacagaa ttggatgcca
     6781 tgaagcgtct tttcctgttc tcgtgcctgc ttggtctcct cgcttcgccg gttgcctcgt
     6841 cgtcgccggc agctgttccc caggagccgg tctcaacccg ttccgccacg ccggcacccg
     6901 ccccgcggcc gtcgctcgtc gtcgtcctcg tcgtggacca gatgcgcgcc gactacatcg
     6961 accagtacgg cgacacct
//
LOCUS       DAOPQX010000192         6859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12209_length_6859_cov_4.130218, whole genome shotgun sequence.
ACCESSION   DAOPQX010000192 DAOPQX010000000
VERSION     DAOPQX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12209_length_6859_cov_4.130218"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PKK95_10795"
     CDS             complement(<1..1635)
                     /locus_tag="PKK95_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC18749.1"
                     /translation="MSEMVLLGDEAVALAGAHAGLTAAYAYPGTPSTEILEYLIRRAR
                     THGGPRAAWCANEKTAYEAALGVSFAGRRAMTSMKHVGLNVAADPFMNSAIVSINGGL
                     VVVSADDPGMHSSQNEQDSRCYAEFARIVCLEPANQQEAYEMTREAFDLSERFRIPVL
                     LRLVTRLAHSRAVVQVSEPRPENEVRKADTPASWILLPGNARVQWRALLARQGEFVEW
                     SNTARWNRLELNPSRRDLGVITTGIARNYYVENLDDLGWRPSHLHVGAYPAPVALVRQ
                     LAAHVNRLLVLEEGYPFLERQLRGLLPPALPIAGKESGELPPDGELTPDLVRTALGLP
                     DRPSLAAGGAPLPNRPPQLCAGCPHGDSFDAIKKAVAEFAGAASAITSDIGCYTLGAL
                     PPYNAVESCVCMGASVGMAKGAAEAGLKPAIALIGDSTFLHSGVTPLMDAVKADTDMT
                     LIILDNETTAMTGGQPTILPSSRLEPLVLGLGVDPAHVHVLEAHHRHAERNREVIHRE
                     LAHPGLSVIIAVRECIETLKAKKRSKGLGAGGWGLGA"
     gene            complement(1753..2607)
                     /locus_tag="PKK95_10800"
     CDS             complement(1753..2607)
                     /locus_tag="PKK95_10800"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOC18750.1"
                     /translation="MRHAAIALLLVTLGPLAGCSPEPPGREYRITGHVLAVKADLTEI
                     TMRHDDIPGFMPAMTMPFKVKDPALLKGRQVGDLVEGTLVVTGEDAWLSRLEKTGWAP
                     FEEPAETARPPVDPVDPGEMVPDETFIDQDGRALRLSELRGSNVLLTFIYTRCPLPDF
                     CPRMDRQFASVQRAVEAGETKHPVRLLSVSFDPDFDTPAVLREHAARVGANPRLWKYV
                     TAPRDRVDAFGARLGLDIIRNPKNASDITHNLRTAVIDPEGRLVTVFGSDWTPELALR
                     ALAPAAPR"
     gene            2808..3143
                     /locus_tag="PKK95_10805"
     CDS             2808..3143
                     /locus_tag="PKK95_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18751.1"
                     /translation="MGRRLYVGNLPYKTTDEDLRALFSQAGGVDNVQVMRDNITGRAR
                     GFGFVEMVTDEDAQKAVAQFHQYVLDGRALVVNEARPKTPGGGRSFAGAGRSDYGDDF
                     GRGSREPRW"
     gene            3422..5044
                     /locus_tag="PKK95_10810"
     CDS             3422..5044
                     /locus_tag="PKK95_10810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOC18752.1"
                     /translation="MRTRSIWLTLTLLALLTAAPSASPQQKQKPGRPGARSAADVRLD
                     GLKAEVAREIDGLQVLTQQMVDSLFSFGEPGFQEFETKRYCTAILREHGFAVEEDVAG
                     MPTGWLASWGSGKPVIALGSDVDGLLETSQKPGVLEHAPLVEGAPGHGEGHNSGIPLS
                     ITAAIALKRVMEREKIGGTIKVWPGVAEELIAGKAQFVKAGVFDGVDAVLFSHVSSGF
                     GVSWGAASGTGLVSVEYTFTGTSAHAGGAPWGGRSALDAVELMDVAWNFRREHLRPSH
                     RSHSVITNGGGQPNVVPPTASVWYYFREIDYPHIKELHELGDTMAKAAAMMTGTTVSS
                     RLLGSAWPQHFNKPVAEATYANIERVGMPSWSDDDQAFAKAVQRLVKAREQGLSTEVG
                     KLGQPVKPEDNRGGGSDDIGDVSWTVPTITLRYPANIPGLPGHHWASAIAMATPVAHK
                     GVTAGAKVEAMTALDLLLKPELVKQAWEYFRNEQTKETTYQPLIGPGDKPAVWLNEKL
                     MRELRPTMQKFYYDPARYKTYLEQLGIPYPPK"
     gene            complement(5048..6529)
                     /locus_tag="PKK95_10815"
     CDS             complement(5048..6529)
                     /locus_tag="PKK95_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18753.1"
                     /translation="MPRSRFTSPACAGWVVAFAVLALLNAGGYRYGVTDQAFYIPAIA
                     RHADPSLFPRDWAMLGAQDQLNAFTPIAGWLVASGLPLPALFLALYAATLALLGAGAL
                     ALGARLGQSRWTAVAILAALTLRHAVALGAVNTLEGYTHPRMLAFGAGVLALAALLAG
                     RRLAVVALVAAAGVVHPTTGIWFGVVAGMGLAVADHGWRPWLLAAAGSAFVLGAWALI
                     AGPLSPRLVRMDPGWLSVLASKRYLFPDRWPATEWLLVAAYASVIALVYWMRRRRGLA
                     STYETGLVCGIGALAAIFAASLPLNAARVAFFVQLQVPRVLWIVDLVAVAYLVWWLSE
                     AGRRARAVALVILALSLGRGTYVTFVEHPERALVQFDLAATEWNRSLQWIDANTPRDA
                     LVAADPGHAWRHGTSVRVGGRRDVLLEESKDTAMAMYSRESALRVLDRIRALDDFGGL
                     TSDRARHLAARYGASVLAIDRDLDLPLLHRQGPFRIYALIPDP"
     gene            6577..>6859
                     /locus_tag="PKK95_10820"
     CDS             6577..>6859
                     /locus_tag="PKK95_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18754.1"
                     /translation="MLDTGGGAGQITHVMNEAPELRAPAAPSAAASRRRTLSDQELVQ
                     SLDWLIQMRWLAGSGVLVATFFAGVVLGLSVPALPLYVIGFAILAYNAAL"
BASE COUNT         1079 a         2320 c         2510 g          950 t
ORIGIN      
        1 agcccctagc ccccagcccc ccgcccccag ccccttgctc ctcttcttcg cctttagcgt
       61 ctcgatgcac tcgcggaccg cgatgatcac cgacagcccg gggtgcgcca gctcgcggtg
      121 gatgacctcc cggttccgct ccgcgtggcg atggtgcgcc tcgagcacgt ggacgtgggc
      181 cgggtccacg ccgaggccga ggaccagcgg ctccagccgg gaggacggca ggatggtcgg
      241 ctgtcctccc gtcattgccg tcgtctcgtt gtcgaggatg atgagcgtca tgtcggtgtc
      301 ggcctttacc gcgtccatca gcggcgtgac gccggagtgc aggaacgtgc tgtcgccaat
      361 cagcgcgatg gccggcttga ggccggcctc ggcggccccc ttcgccattc ccaccgaggc
      421 tcccatgcag acgcacgact ccacggcgtt gtagggcggc agcgccccca gcgtgtagca
      481 gccgatgtcg gaggtgatgg ccgaggccgc gccggcgaac tcggccaccg ccttcttgat
      541 ggcgtcgaac gagtcgccgt gggggcagcc ggcgcagagt tgcggcgggc ggttgggcag
      601 cggcgccccg ccggcggcca gcgacgggcg gtcgggcagg ccgagggcgg tccgcaccag
      661 gtcgggcgtc agctcgccgt cgggcggcag ctcgcccgac tccttgccgg cgatcggcag
      721 ggcgggcggc agcagcccgc gcaactggcg ctcgaggaac gggtacccct cctcgagcac
      781 gagcagccga ttcacgtgcg ccgccagctg ccgcaccagc gccaccggcg ccgggtaggc
      841 gccgacgtgc aggtgcgagg ggcgccagcc aaggtcgtcg aggttctcga cgtagtagtt
      901 gcgggcgatg ccggtggtga tcaccccgag gtcgcgccgc gacgggttca gctcgaggcg
      961 gttccagcgg gcggtgttcg accactcgac gaactcgccc tgccgggcga gcagcgccct
     1021 ccactgcacg cgcgcgttgc cgggcagcag gatccaggag gccggcgtgt cggccttgcg
     1081 cacctcgttc tcgggccgcg gctcgctcac ctggacgacc gcccggctgt gggccagccg
     1141 ggtgaccagc cgcagcagca ccgggatgcg gaagcgctcg gacaagtcga acgcttcgcg
     1201 cgtcatctcg taggcttcct gctggttggc cggctcgagg cagacgatgc gggcgaactc
     1261 ggcgtagcag cggctgtcct gctcgttctg cgagctgtgc atccccgggt cgtcggccga
     1321 aacgaccacc aggccgccgt tgatcgagac gatcgccgag ttcatgaagg ggtcggcggc
     1381 cacgttcagc ccgacgtgct tcatggaggt catcgcgcgg cggccggcga acgacacgcc
     1441 gagcgctgcc tcgtaggcgg tcttctcgtt ggcgcaccag gccgcgcgcg gccctccatg
     1501 cgtgcgggcg cgacggatca ggtattcgag gatctcggtg gacggcgtcc ccgggtaggc
     1561 gtaggccgcc gtgaggccgg cgtgcgcgcc cgcgagggcg acggcttcgt caccgagcag
     1621 gaccatctcg ctcatctgtc agtctcccga gggggccgta ccaaaagcgt gaagaacgtc
     1681 acagggaggg ggccgtacca agaacgtgaa agacttcacg cttttggtac ggcccccggg
     1741 gtgtgagcgt ggtcagcggg gagcggccgg ggccagggcc cgcagcgcca gctcgggtgt
     1801 ccagtcgctg ccgaacacgg tgaccagccg gccttcgggg tcgatgacgg ccgtgcgcag
     1861 attgtgggtg atgtcggacg cgttcttggg attgcggatg atgtcgaggc cgagccgggc
     1921 gccaaaggcg tccacacggt cgcgcggggc ggtgacgtac ttccagagcc gggggttcgc
     1981 gcccacgcgg gcggcgtgct cgcgcagcac cgcgggcgtg tcgaagtcgg ggtcgaagct
     2041 caccgacagc aggcgtaccg ggtgcttcgt ctcgccggcc tcgacggcgc gctgcaccga
     2101 ggcgaactgc cggtccatcc gggggcagaa gtcgggcagg ggacaccgcg tgtagatgaa
     2161 ggtgaggaga acgttcgacc cgcgcagctc cgacaggcga agcgcgcggc cgtcctggtc
     2221 gatgaaggtc tcgtcgggca ccatctcgcc cggatcgacg ggatcgaccg gggggcgggc
     2281 ggtctcggcc ggctcctcga agggcgccca tcccgtcttc tcgaggcgcg acagccaggc
     2341 gtcctcgccg gtcaccacga gcgtcccctc gacgaggtcg ccaacctggc ggcccttcag
     2401 cagcgccggg tccttcacct tgaagggcat cgtcatcgcg ggcatgaagc cggggatgtc
     2461 gtcgtgccgc atcgtgatct cggtgaggtc ggccttcacg gcgaggacat ggccggtgat
     2521 gcggtactcg cgccccggcg gttcggggct gcaccccgcg agcgggccca gggtcaccag
     2581 gagcagggcg atggcggcat gtcgcatggg gcgtccgggc cgggaggggc gaaacgttac
     2641 tatgctatca ggattttggg atatactctc gcagccaagt ggaagcccgc ccgtttgcct
     2701 gcgcatccgt gttcgggccg agtgagcttt cccgagcgtg tcccgagcgc gcgggcccct
     2761 cgtcgtaccg tcagtgtcgc gcagattacg caggagtgag tggaagcatg ggtcggagac
     2821 tgtacgtcgg caatcttcct tacaagacca cggacgaaga cctgcgggcg ctgttcagcc
     2881 aggcgggagg cgtggacaac gtccaggtga tgcgtgacaa catcaccggc cgagcgcggg
     2941 gcttcggctt cgtggagatg gtgaccgacg aggacgccca gaaggcggtg gcgcagttcc
     3001 accagtacgt gctggatggc cgggcgctgg tcgtcaacga ggcgcggccg aagaccccgg
     3061 gcggcggccg ctccttcgcg ggcgccggac ggtcggacta cggcgacgac ttcgggcgcg
     3121 gcagccgcga gccgcgctgg tagccttccg gcgaggggcc gatccccacg gcccctgctc
     3181 cccagccccc aaccgcccgc tttgaaagag gcgactcagc cgagtcgccc tacgtgtgtc
     3241 cgcgacgaac cacgaaggac accacagaga acacaaagaa cacaaagaaa tgcggccgcc
     3301 gaaggccggt gaccgctggc acctcttctg tgatcttcgt ggttgatccg ttggggcacg
     3361 cggtgcggcg tgactggctc cgtggtatac atggcccggc ccgccctccg aggagatgtc
     3421 catgcgcaca cggtcgatct ggctgaccct cacgctgctg gcgctgctca ccgcggctcc
     3481 ctcggcatcc ccgcaacaga agcagaagcc cggccgccca ggggcccgct cggccgccga
     3541 cgtgcgcctc gacggcctga aggccgaggt cgcaagggag atcgacggcc tgcaggtgct
     3601 cacgcagcag atggtggaca gcctcttctc cttcggcgag cccggcttcc aggagttcga
     3661 gaccaagcgc tactgcaccg cgatcctgcg cgagcacggc tttgccgtgg aggaggacgt
     3721 ggcgggcatg ccgaccggct ggctggcgtc gtgggggagc gggaagccgg tcatcgcgct
     3781 cgggtcggac gtggacgggc tgctcgagac ctcgcagaag ccgggcgtcc tcgagcacgc
     3841 gccgctggtc gagggggcgc cggggcacgg cgagggccac aactcgggga tcccgctcag
     3901 catcaccgcc gccatcgcgc tcaagcgcgt gatggagcgg gagaagatcg ggggcaccat
     3961 caaggtctgg ccgggcgtcg ccgaggagct gatcgccggg aaggcgcagt tcgtcaaggc
     4021 tggcgtcttc gacggcgtgg acgcggtgct cttctcgcac gtgtcgtccg gcttcggcgt
     4081 cagctggggc gcggcgagcg ggaccggcct ggtctcggtc gaatacacgt tcaccgggac
     4141 cagcgcccac gccggcggcg cgccgtgggg cggccgcagc gcgctcgacg cggtcgagct
     4201 gatggacgtc gcgtggaact tccgccgcga gcacctgcgg ccctcgcacc gctcgcacag
     4261 cgtgatcacc aacggcggcg gccagccgaa cgtggtgccg cccacggcga gcgtctggta
     4321 ctacttccgg gagatcgact acccccacat caaggaactg cacgagctgg gcgacacgat
     4381 ggcgaaggcg gccgcgatga tgaccgggac gacggtttcg tcgcgactac tggggtcggc
     4441 ctggccgcag cacttcaaca agcccgtggc cgaggccacc tacgcgaaca tcgagcgggt
     4501 cggcatgccc tcgtggagcg acgacgacca ggcgttcgcc aaggcggtcc agcggctggt
     4561 gaaggcgcgc gagcaggggc tgtccaccga ggtggggaag ctgggccagc cggtcaagcc
     4621 cgaggacaac cgcggcggcg gctccgacga catcggcgac gtctcgtgga cggtgccgac
     4681 catcaccctt cgctacccgg cgaacattcc cgggctgccc ggccaccact gggcctcggc
     4741 catcgcgatg gccactccgg tcgcgcacaa gggcgtgacc gccggcgcca aggtggaggc
     4801 gatgacggcg ctcgacctgc tgttgaagcc ggagctggtg aagcaggcgt gggagtactt
     4861 ccgcaacgag cagacgaaag agaccaccta ccagccgctc atcgggcccg gcgacaagcc
     4921 ggcggtctgg ctgaacgaga aactgatgcg cgagctgcgc cccacgatgc agaagttcta
     4981 ctacgacccg gcgaggtaca agacgtatct ggagcagttg gggattccgt atccgccgaa
     5041 gtagggatca gggatcgggg atcagggcgt agatccggaa gggcccctgc cggtggagga
     5101 ggggcaggtc gaggtcgcgg tcgatggcca gcaccgaggc gccgtagcgg gcggcgaggt
     5161 ggcgcgcgcg gtcggaggtc agcccgccga agtcgtcgag cgcgcggatt cgatcgagaa
     5221 ccctcagcgc gctttcccgt gagtacatcg ccatcgcggt gtccttgctt tcctcgagca
     5281 ggacgtcgcg ccggcccccc acccgcacgc tggtgccgtg gcgccaggca tgcccgggat
     5341 cggccgccac cagcgcgtcg cggggagtgt tggcgtcaat ccactgcagc gagcggttcc
     5401 actccgtggc tgcgaggtcg aactgcacca gcgcccgctc ggggtgctcg acgaaggtga
     5461 cgtacgtccc tcgcccgagc gacagggcca ggatcaccag cgccacggcc cgagcccggc
     5521 ggccggcttc cgagagccac cacaccaggt acgccaccgc gaccaggtcc acaatccaca
     5581 gcactcgcgg cacctgcagc tggacgaaga acgcgacgcg cgcggcattc agcggcaggc
     5641 tggccgcgaa gattgccgcg agcgcgccga tgccgcacac gagtccggtc tcatacgtgc
     5701 tcgccaaacc tctgcggcgc cgcatccagt agacgagagc gatcaccgag gcgtaggcgg
     5761 ccactaggag ccactcggtg gcgggccagc gatcggggaa gagatagcgc ttcgaggcga
     5821 gcaccgacag ccagccgggg tccatccgca cgaggcgcgg cgagaggggg ccggcaatca
     5881 gggcccacgc cccgaggacg aaggccgatc ccgcggcggc cagcagccag ggacgccagc
     5941 cgtggtcggc aaccgcgagg cccattcccg cgacaacccc aaaccagatg ccggtcgtcg
     6001 ggtgcacgac gccggccgcg gcaaccagcg cgacgaccgc gagccgccgg cccgcgagca
     6061 gggcggcgag cgccagcaca ccggcgccga aggccagcat ccgggggtgc gtgtaccctt
     6121 cgagcgtatt gacggcgccg agcgccacgg catgcctgag cgtgagggca gccagaatgg
     6181 cgacggccgt ccagcgcgac tggccgaggc gggcgccgag ggcgagcgcg ccggcgccga
     6241 gcagtgcgag cgtggccgcg tagagcgcga ggaagagggc cgggagcggc aggcccgacg
     6301 ccaccagcca gccggcgatt ggcgtgaacg cgttcagctg gtcctgcgcg ccgagcatcg
     6361 cccagtcgcg ggggaagagc gacggatcgg cgtggcgggc aatcgcgggg atgtagaagg
     6421 cctggtcggt gacgccgtag cggtagccgc ccgcgttcag cagcgcgagg acggcgaacg
     6481 ccaccaccca tcccgcgcaa gccggcgacg tgaagcgaga gcgcggcacg ccggtgactc
     6541 tagcacggct ctccgttcgt tgaatccgtt cgttcaatgc ttgacacggg cggcggtgcg
     6601 ggccagatta cccacgtcat gaacgaggcg cccgagcttc gtgctcccgc cgctccgtcc
     6661 gccgcggcat cccgcagacg gacgctgtcg gaccaggaac tggtccagag cctcgactgg
     6721 ctcatccaga tgcggtggct ggcgggctcg ggcgtcctcg tggccacctt cttcgccggc
     6781 gtcgtgctcg ggctctcggt tcccgccctc ccgctgtacg tgatcggctt tgcgatcctc
     6841 gcgtacaacg ccgccctcg
//
LOCUS       DAOPQX010000193         6817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12299_length_6817_cov_4.072907, whole genome shotgun sequence.
ACCESSION   DAOPQX010000193 DAOPQX010000000
VERSION     DAOPQX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12299_length_6817_cov_4.072907"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PKK95_10825"
     CDS             complement(<1..253)
                     /locus_tag="PKK95_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18755.1"
                     /translation="MTEIILHEVGLRDGLQIEKATVPTSQKIAWVQALDAAGVDMVQV
                     GSFVHQQKVPQMADTDDLFRHFTDPAHRPAKARLTALVLN"
     gene            complement(262..1380)
                     /locus_tag="PKK95_10830"
     CDS             complement(262..1380)
                     /locus_tag="PKK95_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOC18756.1"
                     /translation="MAKYRIAWLPGDGIGVEVLEAAKIVLDRLSLDAEYIHGDIGWDF
                     WCREGDAFPARTIELLRNVDAAMFGAITSKPARAAEAELAPDLRGKGLSYRSPIVRMR
                     QLFDLYICLRPCKAYPGNPLNYKEGLDLVVFRENTEDLYSGVEFSPVPEALAATLASL
                     AKPFGTFKGLAPDEYAISCKINTRKGSERIVRAAFDFARKFGRKKVTLVHKANVVRAS
                     DGLFLEMARQVAKGYPEIATDEANIDAMTMWLLKNPFNYDVLVAPNLYGDIVSDLCAQ
                     MVGGLGFGCSGNIGDKLAVFEPTHGSAPKHAGFYKANPIATILAAKMMLDWLGETTKA
                     CALEQATAAVIAEGKVRTYDMGGSSKTLEMAQAIAGKL"
     assembly_gap    1825..1924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1947..2399
                     /locus_tag="PKK95_10835"
     CDS             1947..2399
                     /locus_tag="PKK95_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOC18757.1"
                     /translation="MALVRWDPFRELSDMQDRMGRLLGEFYGRGGADDVMRRGAWVPP
                     VDIYDSGKGELVIKAELPDMKREDIHLTVENNTLTISGEKRMDEELRDEQCHRVERTY
                     GRFTRSFSLPPTVDPGKVSADYKAGVLTVRLPMREEAKPRQIQVQVEE"
     gene            complement(2439..2672)
                     /locus_tag="PKK95_10840"
     CDS             complement(2439..2672)
                     /locus_tag="PKK95_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18758.1"
                     /translation="MSEAWTVILGALLGITVLAVFLLAVLIGFSLFGDSTKFRSRKNS
                     RTIRDLDEGLGGTSAYLPPDAPRGTVDQLAGSK"
     gene            complement(2669..2959)
                     /locus_tag="PKK95_10845"
     CDS             complement(2669..2959)
                     /locus_tag="PKK95_10845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOC18759.1"
                     /translation="MVDENTINEETPETTPAAPEPPAPPIESRFLFVDVAALRAKQLR
                     RGARPRLAALANPETAPDVPHKAERIAMEEVRQGLVHYELPEAKPQDEEQPA"
     gene            3084..3419
                     /locus_tag="PKK95_10850"
     CDS             3084..3419
                     /locus_tag="PKK95_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18760.1"
                     /translation="MLELLSVLVMLALVGVVLAVALALLKLVVMLVLLPVKLALGLVW
                     IPFKLAGWALKLLLLPVALVAGLVLLVLTVVGGLVALAVPLIPLAFGAVVIWAIVRAI
                     SRPEPIRTV"
     gene            complement(3454..4914)
                     /gene="gatA"
                     /locus_tag="PKK95_10855"
     CDS             complement(3454..4914)
                     /gene="gatA"
                     /locus_tag="PKK95_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOC18761.1"
                     /translation="MPPDLRRPLTALAIRNAVRAGRLTAARACRAALDAIEADEPRLH
                     AYITVCADRAMEAARRLDADPARLARLPLAGVPVAIKDNLCTAGLPTTAGSRMLEAFA
                     PPYDATAVRRLEEAGAIVVGKTNCDEFGMGASTEYSAFGPTRNPIDPSRTPGGSSGGS
                     AATVASGAVPIALGSDTGGSVRQPAGFCGVVGLKPTYGRVSRYGLIAFASSLDQVGIL
                     SGTARDAALALQAIAGADAADATAARQPVPDYAAAVEGPATGVRVGVPRALIGEDVDD
                     EVRVRFDEACDALRALGLEVREVSLPHARHAVPVYHLVASAEASANLARYDGVRYGLR
                     APHDGSITGMYAATRGAGFGREVKRRIMLGTYALSAGYYEAYYAKGQQVRALIRAGFE
                     AAFREVDLVALPTAPTPAFRLGERLDDPVRMYLADLFTAGPSLAGLPAVSVPCGATGE
                     GLPVGLQLVGRMFDEAGLLRVANAFQQDAAARNGVA"
     gene            complement(4918..5217)
                     /gene="gatC"
                     /locus_tag="PKK95_10860"
     CDS             complement(4918..5217)
                     /gene="gatC"
                     /locus_tag="PKK95_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOC18762.1"
                     /translation="MSQALPEREVERIARLARLAPTPVERRRLAEELGQILAFAAAVA
                     SVDTSGVEPGVDWLVQEPLERPDQPRPSLAADAALANAPEHADGLVLVPRVLPRD"
     gene            complement(5231..5833)
                     /gene="amrA"
                     /locus_tag="PKK95_10865"
     CDS             complement(5231..5833)
                     /gene="amrA"
                     /locus_tag="PKK95_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOC18763.1"
                     /translation="MLSPRERRLLLQYARDTLAAYFQGEPPPPPPSLENPTRHSGAFV
                     TLEVGGDLRGCIGYPGSSQPLDRVVAECAVAAASEDPRFTPLRRREMDALEIEISVLT
                     PIVPVEDLNDIVVGRDGLVVSDGRRRGLLLPQVAVEHGWDRDTFLAHTCLKAGLRADA
                     WKTGARVLRFQAEVFSERGEHATASGQEPGGEASGRPPGS"
     gene            complement(5852..6694)
                     /gene="amrB"
                     /locus_tag="PKK95_10870"
     CDS             complement(5852..6694)
                     /gene="amrB"
                     /locus_tag="PKK95_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOC18764.1"
                     /translation="MPKPEGPVRRAAVAGSWYPGSADALASEVDRHLEAASPGLPRGT
                     IRGLVAPHAGLMYSGPVAAWAYRAVQDDPFEVAVLVGPSHRLGFEGVALYPRGAWDTP
                     LGPAPIAEDAAAAILAAGSPAVHASEAAHAREHSLEMQLPFLRRVLPATPIVPLLMGL
                     QTPETIAALGSALAVALEGRRALLVASSDLSHYMDSRAAARMDRQVLDLVAAFDDEGL
                     VRLLRTRPDHACGGGPLASVMRAARALGARAARVLRYGDSGDTSGDKSAVVGYMAAAF
                     GAFA"
BASE COUNT          980 a         2293 c         2416 g         1028 t
ORIGIN      
        1 cgttcagcac gagcgcggtg agccgggcct tcgccgggcg gtgggcagga tcggtgaagt
       61 gcctgaacag gtcgtcggtg tcggccatct gcggcacctt ctgctggtgc acgaacgagc
      121 cgacctgcac catgtcgacg ccggccgcgt cgagcgcctg cacccaggcg atcttctgcg
      181 aggtcggcac ggtggccttc tcaatctgca ggccgtcccg aaggccgacc tcgtggagga
      241 tgatctcggt catcccgtct cctagagttt cccggcgatc gcctgcgcca tctcgagcgt
      301 cttgctcgag ccgcccatgt cgtaggtgcg gaccttgccc tcggcgatga cggcagcggt
      361 cgcctgctcg agcgcgcaag ccttcgtggt ctcgccgagc cagtcgagca tcatcttcgc
      421 ggcaaggatc gtggcgatcg ggttcgcctt gtagaaccct gcgtgcttcg gcgccgagcc
      481 gtgtgtcggc tcgaacaccg cgagcttgtc gccgatgttc cccgagcagc cgaagccgag
      541 gccgccgacc atctgcgcgc acaggtccga cacgatgtcg ccgtacagat tcggcgccac
      601 cagcacgtcg tagttgaacg ggttcttgag cagccacatc gtcatggcgt cgatgttggc
      661 ttcgtcggtg gcgatctccg ggtagccctt ggccacctgg cgggccatct cgaggaacag
      721 cccgtcactg gcgcggacga cgttcgcctt gtgcacgagg gtgaccttct tgcggccgaa
      781 cttacgggcg aagtcgaagg ccgcgcggac gatgcgctcg gatcccttgc gggtgttgat
      841 cttgcacgag atggcgtact cgtcgggcgc gagacccttg aacgtgccaa agggcttggc
      901 gagcgaggcg agcgtggctg cgagcgcctc ggggaccgga gagaactcca cgccggaata
      961 caggtcctcg gtgttctcgc ggaagacgac gagatcgagg ccttccttgt agttgagcgg
     1021 gttgcccggg taggccttgc agggccggag gcagatgtag agatcgaaca gctgccgcat
     1081 gcgcacgatc ggcgagcggt agctcaggcc cttgcccctc aagtcgggcg ccagttccgc
     1141 ctcggcggcc ctggccggct tcgaggtgat cgcgccgaac atcgccgcgt cgacgttccg
     1201 gagcaactcg atggtgcgag ccgggaaggc atcgccctcg cgacaccaga agtcccaccc
     1261 gatgtcgccg tggatgtact cggcgtcgag gctgaggcgg tcgaggacga tcttcgcggc
     1321 ctcgagaact tccacgccaa tcccgtcgcc cggcagccag gcaatcctgt acttcgccat
     1381 tgccaacacc tcgtcgtgtt cggggaagag cctatcgcca gtcctggttc cgagaaggcg
     1441 ggggcgcgaa ccttcacagt ctatatcggg tgccacctcg aggggtaggg agaagaacgg
     1501 ccgctacgcc aaatttcctt tggcttccag tcgtattgtc aaacttctct acggtatctg
     1561 gtgtcatcga cggccgtttt actggatgac gagatccggc tccggaagct gggtccatcc
     1621 cgccaagacc cgcaggaggc cgtacggtgc gttctggtga gtgttgataa ctcatatatg
     1681 atgcttgaca tgcactcaaa tcacacctac actcgtctca ggttctgttc ggggcccgct
     1741 ccaaccacgg cccgaagtca ggcccgggcc gttcaagctc aggagggacg tatggcactg
     1801 gtacgttggg atccgttccg ggaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnncgttca agctcaggag ggacgtatgg cactggtacg ttgggatccg ttccgggaac
     1981 tgtcggacat gcaggaccgc atgggccgcc tcctcgggga gttctacggg cgtggcgggg
     2041 ccgacgacgt gatgcgccgc ggcgcctggg tgccgccggt cgacatctac gacagcggca
     2101 agggcgagct ggtgatcaag gccgagctgc ccgacatgaa gcgcgaggac atccacctga
     2161 cggtcgagaa caacacgctc accatcagcg gcgagaagcg gatggacgag gaactgcgcg
     2221 acgagcagtg ccaccgcgtc gagcggacgt acggccgctt cacgcgctcg ttctcgcttc
     2281 cgcccacggt cgatcccggg aaggtgtcgg ctgactacaa ggccggcgtg ctgacggtgc
     2341 ggctgcccat gcgcgaggag gcgaagcccc gtcagatcca ggtgcaggtg gaggagtagg
     2401 ggctagggcc agggcctggg gccaggagct acggcggcct acttcgatcc ggcgagctgg
     2461 tccacggtgc cgcgcggcgc gtcgggcggc aggtaggcgg aggtgccgcc caggccctcg
     2521 tcgaggtcgc gaatcgtgcg cgagttcttc cgcgacctga acttggtgct gtcgccgaac
     2581 agcgagaagc cgatcagcac cgcgagcagg aagaccgcga ggaccgtgat gcccaggagg
     2641 gcgccgagga tgacggtcca ggcctcgctc acgccggctg ctcctcgtcc tgcggcttgg
     2701 cctcgggcag ctcgtagtga acgaggccct ggcgcacctc ctccatcgcg atccgttcgg
     2761 ccttgtgcgg cacgtcgggc gcggtttcgg ggttggccag ggcggccagc cgcggccgcg
     2821 ccccccgtcg cagctgcttg gcgcgcaggg cggccacgtc cacgaagagg aaccggctct
     2881 cgatgggcgg ggccggcggc tcgggcgcgg cgggggtggt ttcgggtgtt tcttcgttga
     2941 tcgtgttttc gtctaccaca atccctcaga gtagcggggc tggcgccgcg ccgcaagcaa
     3001 aatccgtccc tgaaacttca cggcccccgt ttccgtagga tccggtaggc cgggggcgca
     3061 ccccgcccgg ccaggagacg gccatgctgg agctgttgtc ggtgctcgtg atgctggcgc
     3121 tcgtcggggt ggtcctcgcg gtggcgctgg ccctgctgaa gctcgtcgtg atgcttgttc
     3181 tgctcccggt caagctcgcg ctcgggctcg tctggatccc gttcaagctg gcaggctggg
     3241 cgctgaagct tttgctgctc cccgtggcgc tcgtcgcggg cctcgtcctg ctggtgctga
     3301 ccgtggtggg cgggctcgtc gcgcttgccg tgccgctgat cccgctcgcc ttcggcgcgg
     3361 tcgtgatctg ggccatcgtt cgcgcgatca gccgtccgga gccgatccgg acggtgtagt
     3421 agcacccccc gcacgcccgc agccggccct ccttcaggcc acgccgttcc gcgccgcggc
     3481 gtcctgctgg aacgcgttcg ccacgcgcag cagccctgcc tcgtcgaaca tccgcccgac
     3541 cagttgcagt ccgaccggta gcccctcgcc agtagcaccg caggggaccg agaccgccgg
     3601 aaggcctgcc agcgacggcc ccgccgtgaa gaggtcggcg aggtacatcc gcaccgggtc
     3661 gtcgaggcgc tcgcccaggc ggaacgccgg ggtgggcgcg gtgggcagcg cgacgagatc
     3721 cacctcgcgg aacgctgcct cgaagccggc ccgtatcaac gcccgcacct gctgtccctt
     3781 cgcgtagtac gcctcgtagt agcccgcgct cagcgcgtag gtgccgagca tgatgcggcg
     3841 cttgacctcg cgcccgaagc ctgcgccgcg cgtggcggcg tacatgccgg tgatcgagcc
     3901 gtcgtgcggc gcccgcaagc cataccgcac cccgtcgtag cgcgccaggt tcgcgctcgc
     3961 ctctgcgctg gccaccaggt ggtagaccgg gacggcgtgt cgcgcatgcg gaagcgacac
     4021 ctcccgcacc tcgaggccaa gggcgcgaag ggcgtcgcac gcttcgtcga accggacgcg
     4081 cacctcgtcg tccacgtcct cgccgatgag cgcgcgcggc acgccgacgc gcacgccggt
     4141 tgcggggccc tcgaccgcgg cggcgtaatc gggaacgggc tgccgtgcgg cggttgcgtc
     4201 ggcggcgtcg gcgccggcga tcgcctggag cgccagggcg gcgtcgcgcg cggtgcccga
     4261 aaggatgccc acctggtcga gcgacgaggc gaacgcgatc agtccgtagc gcgacacgcg
     4321 gccgtaagtc ggcttcagcc ccaccacgcc gcagaagccg gccggctggc ggaccgatcc
     4381 cccggtgtcc gatccgagcg cgatcggcac ggcgccgctg gcgaccgtgg ccgccgatcc
     4441 cccgctcgac ccgccgggcg ttcgcgacgg atcgatcggg tttctggtgg gtccgaacgc
     4501 cgagtactcg gttgacgccc ccatcccgaa ctcgtcgcag ttggtcttcc cgacgacgat
     4561 ggcgccggcc tcctcgaggc ggcgaacggc ggtggcgtcg tagggcgggg caaacgcctc
     4621 cagcatgcgc gagcctgccg tcgtcggcag gccggccgtg cacaggttgt ccttgatcgc
     4681 caccggcacg ccggccagcg ggaggcgcgc gaggcgcgcc gggtcggcgt cgagccggcg
     4741 ggccgcctcc atcgcgcgat cggcgcagac ggtgatgtag gcgtggagcc gcggctcgtc
     4801 tgcttcgatg gcgtcgagcg ccgcgcggca cgctcgggcg gcggtcagcc gcccggctcg
     4861 aacggcgttg cggatggcga gggccgtgag agggcgccga aggtccggag gcatcgatca
     4921 gtcccgcggg aggacgcgcg gcaccaggac gaggccgtcc gcgtgctcgg gcgcgttggc
     4981 gagggcggcg tcggccgcca gcgacggccg cggttggtcg gggcgctcga ggggttcctg
     5041 gaccagccag tccacccccg gctcgacacc gctcgtgtcc accgaggcaa ccgcggcggc
     5101 gaaggcaagg atctgcccca gttcctcggc aagccggcgc cgctcgacag gggtgggcgc
     5161 caggcgcgca agcctggcaa tccgctccac ttcgcgctcg ggcagcgcct ggctcatgac
     5221 gcggatggta tcacgagccc ggagggcgcc cgctcgcctc gccgccgggc tcctgcccgg
     5281 acgcggtcgc gtgctcgccg cgctcggaga acacttccgc ctggaaacgc aggacgcgtg
     5341 cgccggtctt ccaggcgtcg gcgcgcagcc cggccttcag gcacgtgtgg gcgagaaacg
     5401 tgtcgcggtc ccacccgtgc tcaacggcca cctggggcag cagcaggccc ctgcgccttc
     5461 catcggagac caccaggccg tcgcggccca ccacgatgtc gttcagatcc tcgacgggca
     5521 cgatgggcgt cagcaccgag atttcgatct cgagcgcgtc catctcgcgc cggcggagcg
     5581 gcgtgaagcg gggatcctcg ctggcggcgg cgaccgcgca ctccgctacc acccggtcga
     5641 ggggctgcga cgagcccggg tagccgatgc agccgcgaag gtctccaccg acctcgagcg
     5701 tgacgaacgc gcccgagtgc cgggtgggat tctcgagcga cggcggtggg ggcggcggtt
     5761 cgccctggaa ataggccgcg agggtgtccc tggcgtactg caacaaaagg cgtcgctccc
     5821 tggggctgag catgaatggc tccacgatgt ctcacgcgaa cgcgccgaac gcggcagcca
     5881 tgtaccccac gacggcgctc ttgtcgcccg aggtgtcgcc cgagtcgccg tagcggagca
     5941 cgcgagccgc gcgcgccccg agtgcccgcg cggcgcgcat gaccgacgcc agcggcccgc
     6001 cgccgcaggc gtggtcgggc cgtgtccgca gcaggcgcac cagcccttcg tcgtcgaacg
     6061 ccgccaccag gtcgagcacc tggcgatcca tccgggcggc ggcgcggctg tccatgtagt
     6121 gcgagaggtc gctgctcgcc accagcagcg cgcgccggcc ctccagcgcg accgccagag
     6181 ccgagccgag ggcggcgatc gtctcgggtg tctgcaggcc catcagcagc ggcacgattg
     6241 gcgtcgccgg cagcacgcgg cgcaggaagg gcagctgcat ctcgagcgag tgctcgcggg
     6301 cgtgcgcggc ctccgacgcg tgcaccgccg gcgagccggc ggcgaggatc gccgccgcgg
     6361 catcctcggc gatcggggcc ggcccgagcg gcgtgtccca ggcaccgcgc gggtagagcg
     6421 ccaccccctc gaacccgagg cggtgcgacg gccccaccag cacggccacc tcgaaggggt
     6481 cgtcctgcac cgcgcgatag gcccacgcgg caaccggccc cgagtacatc agccccgcgt
     6541 gcggagcgac caggccgcga atggtaccgc gcggcagccc gggagacgcg gcctcgaggt
     6601 gtcgatcgac ctcggacgcc aacgcgtcgg ccgagcccgg gtaccacgat ccggcgacgg
     6661 cggcccgccg gacggggcct tccggtttgg gcataaaagg atcctactac ttacgagtcg
     6721 tgctgggggt gctggaggtg cagggggtgc tggaggtgct ggtgctggag gtgctggagg
     6781 tgctggaggt gctggtgcag aaggtgctgg tgcagaa
//
LOCUS       DAOPQX010000194         6794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12351_length_6794_cov_5.137558, whole genome shotgun sequence.
ACCESSION   DAOPQX010000194 DAOPQX010000000
VERSION     DAOPQX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12351_length_6794_cov_5.137558"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKK95_10875"
     CDS             complement(<1..532)
                     /locus_tag="PKK95_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOC18765.1"
                     /translation="MAEDPEDPSRQATGVSRRGFIATVGTGAVAAATVAKVGAQAGSA
                     TLPDGAEMVRVALDVNGRTHRLLVEPRWSLLYVLRERLGLTGTKLGCERGECGVCTVL
                     VDDKPQYSCMMLAVEAEGRRITTVEGLMQGEELGPVQQAFVEEDAFQCGYCTPGQVMA
                     VEGLLRATPEPTTEQIR"
     gene            708..2162
                     /locus_tag="PKK95_10880"
     CDS             708..2162
                     /locus_tag="PKK95_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOC18766.1"
                     /translation="MAKAIEGLTPALLWKYFAEISAIPRCSKNETAIARYLLDTAARL
                     GLEAEQDGVGNVLVRKPASPGREGVPMTCLQGHMDMVCEKNKDTVHDFSTDPLELVRR
                     GSFLMANGTTLGADNGIAVATCLAIMEDQALEHGPLEFLFTVDEETGLTGANNLQAGW
                     LRSRTLLNLDSEEEGALYVGCSGGRDSTATWPGGYGHVPEGAVPLLVKVGGLKGGHSG
                     LEIHQGRGNSLKILTRALLALEGLGGRVARMEGGNKRNAIPREAEAVVYVPETVEPEA
                     MASVAALQEAVASELASIDPGVSVTAERAADAPAEVLNAELQHRVLLALAAVPHGVLK
                     MSPDIPGLVETSTNLAILKTDDQAVTVQTSQRSSVQSEIEEAAQMVRAAFELSGAQVT
                     GSDGYPGWKPDLASPILGVARRVYQELYGREPEVKAVHAGLECGIIGEKYPGMDMVSL
                     GPTLEGVHSPDEKIHLDTVGKFWDYVLAILRARS"
     gene            2258..3058
                     /locus_tag="PKK95_10885"
     CDS             2258..3058
                     /locus_tag="PKK95_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18767.1"
                     /translation="MRRPAIQWALAIVITLTSAVWQRMSGPTYPVRGTVQVAGESIGL
                     RLERSHSVSARQPVVVKAPDGALTGEVSWRRYPTQEPWQTLPLERRGDQLRAELPPEP
                     LPPMPPAGKLEYQVRLRRGGDEAAFPAVPAVTRFKGDVRAAVLTPHILAMFVGMLLST
                     RAGLAAVFGGRLRLVTYLTLALLVAGGLVLGPIVQKAAFGAYWTGVPWGWDLTDNKTL
                     IAALAWTFAAWRVGRGGLGARSAVGLAAVVTLVVFAIPHSVWGSEVKW"
     gene            complement(3110..6190)
                     /locus_tag="PKK95_10890"
     CDS             complement(3110..6190)
                     /locus_tag="PKK95_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18768.1"
                     /translation="MNARTTWRQAWIALALTLAVASPASAQYFGQNKVQYKSFDFEVL
                     KTQHFDIYFYPAEREAVRQAAVMAERWYARLSRVLNHELRGAQPLILYASHPEFEQTN
                     AIPGEMGEGTGGVTEMLKRRIVLPLGASLAESDHVIGHELVHAFQFDITSPAGGQSMA
                     SGALRLPLWFIEGMAEYLSIGPVDPHTAMWMRDAAVEEKIPTIRELDQPRYFPYRWGQ
                     AFWSYVTGRWGDRVIGEMLRIGAVTGDPEIAINKVLGLPSDDLSAQWHESLRQLSAAV
                     RQQTRPPSDFGRALQKAKGSGDDDRAMNISPALSPDGRRVMFLSQRDLLSVGLYMADA
                     ETGKVVRKVVDTTFDPEFSSIQFIASAGDWAPDNRRFVFAAVKTGAPQLVIYDADADR
                     IEKEVRFKELGEIFNPSWSPDGRLIVFTALHGGHTDLFLHDLQAGTTRQLTSDAFADL
                     HPSWGPDNRRLAFVTDRFSTNLPRLIPGDYRIALLDIADGSIKEVAGTDRGKNINPQW
                     GPDGGLYFVSDRNGISNVYRTTVEGGTLSQLTNLATGVSGITDLSPAISFASGARRLA
                     MSVYENGSYNLYTMDSAEALAGKPPVALVSVNAAQLPPATRVASQYAELRANPEVGVP
                     AAADPQEEEYRGGLSLDFIGQPYLAAGTDPYSTYVGGGISLFWSDMLGNHNLGTAIQV
                     NSAFTNSVGDVFKNTGVQVAYQNLKHRWNWGVSGGQMPYLTGAFASGLAQVDGQIVGV
                     EEQLIFRQIERGVTGVAAYPFNRVHRVEFTGGYTNIAFDRQIRTTLFDPITGDVLARD
                     TQELESIGGLHLADVSAALVYDSSIFGATSPIAGQSYRLQVTPTVGSLQMNTVLADYR
                     RYLMPVSFYTFATRVVHYGRYGRDSEDDRLMPLFLGYPNLVRGYDYSSFNAQECTPSA
                     TSSCQEFDRLLGSRMLVGNAEFRFPLLRPFGVGSGMYGPVPLELAFFGDAGVAWNKGE
                     SPDGLGGSREWVSSAGVALRANLFGYMIAQIDFVKPFQRPQKGWIFQFSLTPGW"
     gene            complement(6315..6515)
                     /locus_tag="PKK95_10895"
     CDS             complement(6315..6515)
                     /locus_tag="PKK95_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18769.1"
                     /translation="MKAEDAVHDQPAGWMAKNRWKLAFALAAAAGGFWWGRSRRPRFE
                     VDRVSEQWLAEHTFEAGRHPPE"
     gene            complement(6600..>6794)
                     /locus_tag="PKK95_10900"
     CDS             complement(6600..>6794)
                     /locus_tag="PKK95_10900"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOC18770.1"
                     /translation="QVKALWHDGYRPWFPKGPEDESISLIRVRVEEAGYWDAAAGRMV
                     PLAGFVEGTAFHDLQPGIGI"
BASE COUNT         1025 a         2408 c         2304 g         1057 t
ORIGIN      
        1 cacggatctg ctcggtggtc ggctcgggag tcgcgcgcag cagcccctcg acggccatca
       61 cctggccggg cgtgcagtag ccgcactgga aggcgtcttc ctcgacgaac gcctgctgca
      121 ccgggccgag ctcctcgcct tgcatcagcc cctcgaccgt ggtgatgcgg cggccctcgg
      181 cctccaccgc gagcatcatg caggagtact gcggcttgtc gtccaccagg acggtgcaca
      241 cgccgcactc tccgcgctcg catccgagct tggtgccggt gaggccgagc cgctcgcgca
      301 gcacgtacag cagcgaccag cgcggctcca ccagcaggcg gtgggtgcgg ccgttcacgt
      361 cgagggcaac gcgcaccatc tccgccccgt cgggaagcgt cgcagaccct gcctgggccc
      421 caaccttggc gaccgtcgcg gcggcaaccg cgccggtccc gaccgtggcg atgaatccgc
      481 gccggctgac accggtcgcc tgtctggacg gatcttcggg gtcttcagcc atggaaacct
      541 cgtgctcgag accgggccgt cgggaaccgg ccggtggacg gaatcgggaa gatgaccgcc
      601 cggattatag ctcatcacga cttgcccttc cccccgtctt cccgtacgct gacgaggtcc
      661 cggcccgcct cgcgccgagg cgcgcccggc gaaaaggagc gttcgacatg gccaaggcaa
      721 tcgagggact gacgcctgcc ctgctgtgga agtacttcgc cgagatcagc gccatcccgc
      781 gctgctcgaa gaacgagacg gccatcgccc gctacctgct cgacacggcc gcccgcctcg
      841 ggctcgaggc cgagcaggac ggcgtgggca acgtgctggt ccgcaagccc gcgtcgccgg
      901 gccgcgaggg cgtgccgatg acgtgcctgc aggggcacat ggacatggtg tgcgagaaga
      961 acaaggacac ggtccacgac ttctccaccg atcccctcga gctcgtccgc cgcggcagct
     1021 tcctcatggc caacggcacc acgcttggag ccgacaacgg catcgccgtc gccacctgcc
     1081 tcgccatcat ggaggaccag gccctcgagc acggaccgct cgagttcctc ttcacggtgg
     1141 acgaggagac ggggctcacc ggcgcgaaca atctccaggc aggctggctt cgcagccgga
     1201 cgctgctgaa cctcgactcc gaagaagagg gagccctcta cgtcggctgc tccggcgggc
     1261 gcgactccac ggccacctgg cccgggggat acgggcacgt tcccgagggt gccgtcccgt
     1321 tgctcgtgaa ggtgggcggc ctcaagggcg ggcactcggg cctcgagatc caccaggggc
     1381 gcgggaactc gctgaagatc ctcacccgcg cgctgcttgc cctcgagggg ctcggcggac
     1441 gcgtggcccg gatggagggg ggcaacaagc gcaacgcgat cccgcgcgag gccgaggccg
     1501 tcgtgtacgt cccggagacg gtcgaacccg aggcgatggc gtcggtcgcg gcgctgcagg
     1561 aggccgtcgc gtccgagctg gcgtccatcg atccgggagt cagcgtgacg gccgagcggg
     1621 cggcggacgc gccggccgag gtcctcaacg ccgaactgca gcaccgcgtg ctgctcgcgc
     1681 tggcggccgt cccgcacggc gtgctcaaga tgagccccga catccccggc ctcgtggaaa
     1741 cctccaccaa cctcgcgatc ctgaagaccg acgaccaggc cgtgacggtg cagaccagcc
     1801 agcgcagctc cgtgcagtcg gagatcgagg aagccgcgca gatggttcgc gcggcgttcg
     1861 agttgagcgg ggcgcaggtg accgggtcgg acggctaccc gggatggaag cccgacctgg
     1921 cctcgccgat ccttggcgtc gccaggcgcg tctaccagga gctgtacggc cgcgagcccg
     1981 aggtgaaggc ggtgcatgcg gggctcgagt gcgggatcat cggcgagaag taccccggca
     2041 tggacatggt gtcgctcggg cccacgctcg agggcgtgca ctcccccgac gagaagatcc
     2101 acctcgacac ggtcggcaag ttctgggact acgtgctggc catcctgcgg gccaggagct
     2161 agggaccctg gcgcgtagcg cagggcttca accctgcggc gctggcgccg aagctgttac
     2221 gactttcgtt tccgttttcc gttctcgagg actctcgatg cgacgaccgg cgattcagtg
     2281 ggccctggcg atcgtgatca cgctcacctc cgcggtctgg cagcggatga gcggtcctac
     2341 ctacccggtt cgcggcaccg tccaggtggc gggcgagtcg atcggcctgc ggctcgagcg
     2401 gtcgcactcg gtctcggccc gccagcctgt cgtcgtgaag gcgcccgacg gggcgctgac
     2461 gggcgaggtg tcgtggcgcc ggtatcccac ccaggagccg tggcaaacgc ttccgctcga
     2521 gcgccgcggc gaccaactgc gcgccgagct gccgcccgaa ccgctgccgc ccatgcctcc
     2581 tgccggcaag ctcgaatacc aggtgcggct ccggcgcggg ggcgacgagg cggcctttcc
     2641 cgccgtgccg gcggtgaccc gcttcaaggg cgacgtccgg gcggcggttc tcacccctca
     2701 catcctcgcg atgttcgtcg ggatgctgct ctcgacccga gccgggctgg ccgccgtctt
     2761 cggcggccgc ctccggctcg tcacctacct cacgctcgcc ctgctggtcg ctggaggcct
     2821 ggtcctcggc cccatcgtgc agaaggccgc attcggggcg tactggaccg gagtgccctg
     2881 ggggtgggac ctgaccgaca acaagacgct gatcgcggcc ctggcctgga ccttcgcggc
     2941 gtggcgggtc ggaaggggcg ggctgggtgc gcgttccgcc gtgggactgg cggccgtggt
     3001 cactctggtc gtcttcgcga tcccgcactc ggtgtgggga tcagaagtga agtggtgacc
     3061 ttcacggcca ccactccact tcgcactgtc gaaggcagga cttccggccc taccaccccg
     3121 gcgtcaggct gaactggaag atccagccct tctgcgggcg ctggaacggc ttcacgaagt
     3181 cgatctgcgc gatcatgtag ccgaagaggt ttgcgcgcag cgcgacccct gcgctcgaca
     3241 cccactcgcg cgagccgccg aggccgtccg ggctctctcc cttgttccat gccacgccgg
     3301 cgtcgccgaa gaaggccagc tcgagcggca ccggcccgta catcccgctg cccacgccga
     3361 agggccgcag cagcgggaag cggaactcgg cgttgccgac cagcatgcgg gaccccagca
     3421 gccggtcgaa ctcctggcac gaggaggttg cggagggcgt gcactcctgg gcgttgaagg
     3481 agctgtagtc gtagccccgc acgaggttgg ggtatccgag gaacagcggc atcagccggt
     3541 cgtcctccga gtctcggccg tagcggccgt agtgcaccac acgggtggcg aacgtgtaga
     3601 acgacaccgg catgaggtag cgccggtagt cggcgagcac ggtgttcatc tgcagcgacc
     3661 cgaccgtggg cgtgacctgc aggcggtacg actggccggc gatcgggctg gtcgccccga
     3721 agatcgagct gtcgtagacc agggccgcgc tcacgtcggc caggtgcagg ccgccgatgc
     3781 tctcgagttc ctgcgtatcg cgggcgagca cgtccccagt gatcgggtcg aacagcgtcg
     3841 tccggatctg ccggtcgaac gcgatgttcg tgtatccgcc ggtgaactcg acgcgatgca
     3901 cccggttgaa ggggtacgcg gccacgcccg tgacgccgcg ctcgatctgc cggaagatca
     3961 gctgctcctc gaccccgacg atctgtccgt ccacctgggc gaggccgctc gcgaaggccc
     4021 cggtgaggta cggcatctgc ccgcccgaca ccccccagtt ccagcggtgc ttcaggttct
     4081 ggtaggcgac ctgcacgccc gtgttcttga agacgtcgcc gacactgttg gtgaaggcgc
     4141 tgttcacctg gatggcggtg ccgaggttgt ggttccccag catgtcgctc cagaagagcg
     4201 agataccgcc gccgacgtag gtgctgtacg ggtcggttcc cgcggcgagg tacggctgcc
     4261 cgatgaaatc gagcgagagc ccgcccctgt actcctcctc ctgcggatcg gccgccgccg
     4321 gcacgccgac ctcggggttc gcccgcagct cggcgtactg gctcgccacc cgcgtcgccg
     4381 ggggcagctg cgccgcgttc acgctgacca gcgccaccgg cggcttcccg gcgagggcct
     4441 ccgccgagtc catcgtgtac aggttgtagc tgccgttctc gtagaccgac atcgcgaggc
     4501 ggcgggcgcc cgacgcgaag gagatcgcag ggctcaggtc ggtgatcccg ctgacgccgg
     4561 tcgccaggtt cgtcagctgc gacaacgtcc cgccctccac cgtggtccgg tagacgttgc
     4621 tgatgccgtt ccgatccgaa acgaagtaca gcccgccgtc gggcccccac tggggattga
     4681 tgttcttgcc gcggtcggtc cccgcgactt ccttgatcga cccgtcggcg atgtcgagca
     4741 gcgcgatgcg atagtcaccc ggtatgagcc gcgggaggtt cgtcgagaac cggtccgtga
     4801 cgaacgccag gcgccggttg tcggggcccc acgaggggtg caggtccgcg aacgcgtcgc
     4861 tcgtgagctg tcgcgtggtg ccggcctgca ggtcgtgcag gaacaggtcg gtgtggccgc
     4921 cgtgcagcgc ggtgaacacg atgagccttc cgtcgggcga ccacgaggga ttgaagatct
     4981 cccccagttc cttgaagcgg acctccttct cgatgcggtc cgcgtcggcg tcgtagatca
     5041 ccagctgcgg cgcaccggtc ttgacggccg cgaacacgaa gcgccggttg tcgggcgccc
     5101 agtcgcccgc cgaggcgatg aactggatgc tgctgaattc ggggtcgaag gtcgtgtcca
     5161 cgaccttgcg cacgaccttg ccggtctcgg cgtcggccat gtacaggccg accgacagca
     5221 ggtcgcgctg cgacaggaac atcacgcgcc gcccgtcggg gctcagcgcg ggcgagatgt
     5281 tcatcgcccg gtcgtcgtcg ccgctccctt tggccttctg cagcgcgcgg ccgaagtcgg
     5341 acggcgggcg cgtctgctgc cgcacggcgg ccgacagctg gcgaagcgac tcgtgccact
     5401 gcgccgagag gtcgtcggag gggagtccca gcaccttgtt gatcgcgatc tcgggatcgc
     5461 ccgtcaccgc gccgatccgc aacatctcgc cgatgacccg gtcgccccag cggccggtca
     5521 cgtacgacca gaaggcctgc ccccagcggt acgggaagta gcgcggctga tcgagctcgc
     5581 ggatcgtcgg gatcttctcc tcgaccgccg cgtcgcgcat ccacatcgcc gtgtgcggat
     5641 cgaccggccc aatcgacagg tactccgcca tcccctcgat gaaccacagc ggcagccgca
     5701 gcgctcccga cgccatgctc tgcccgcccg cgggcgaggt gatgtcgaac tggaacgcgt
     5761 ggaccagctc gtggccgatc acgtgatcgc tctcggccag cgacgcgccg agcggcagca
     5821 cgatgcggcg cttcaacatc tcggtcacgc cgccggtgcc ctcgcccatc tcgcccggta
     5881 tcgcgttggt ctgctcgaac tcggggtggc tcgcgtagag gatcagcggc tgcgcgcccc
     5941 gcagctcgtg gttcagcacc ctcgagagtc gcgcgtacca gcgctcggcc atcaccgcgg
     6001 cctgccgcac cgcctcgcgc tcggccgggt agaagtagat gtcgaagtgc tgcgtcttca
     6061 gcacctcgaa atcgaagctc ttgtactgga ccttgttctg gccgaagtac tgggcgcttg
     6121 cggggctcgc caccgcgagc gtcagcgcca acgcgatcca ggcttgccgc cacgtcgttc
     6181 gggcgttcat cgggacccct ccgggaggcg cgatcgcatc aatcgtacca gcgaatggag
     6241 agcgattcag cagacaaaca tcagacaaac gaggtgcggc gggaaggcag cgtcgaggcg
     6301 gcccgcgatc gcgcctactc cggcggatgc cgcccggcct cgaaggtgtg ctcagcgagc
     6361 cactgctcgc tgacgcgatc gacctcgaac cgcggccgcc gcgatcggcc ccaccagaac
     6421 ccgcccgcgg ccgccgccag ggcgaacgcc agcttccagc ggttcttcgc catccacccc
     6481 gccggctggt cgtgaaccgc gtcctcggcc ttcatcggcg atcccctctt caagggtcct
     6541 gccagccgcg tcccgtcctt ttcctgtcct ctgccggtcc cacctgtcca cttctagggt
     6601 cagatgccaa tccccggctg gaggtcgtga aatgcggtgc cctcgacgaa gcccgcgagc
     6661 ggcaccatcc ggccggctgc cgcgtcccag taccccgcct cctccacccg aacccggatc
     6721 agcgagatcg actcgtcctc gggccccttc gggaaccacg gccgataccc gtcgtgccag
     6781 agcgccttga cctg
//
LOCUS       DAOPQX010000195         6729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12504_length_6729_cov_4.258016, whole genome shotgun sequence.
ACCESSION   DAOPQX010000195 DAOPQX010000000
VERSION     DAOPQX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12504_length_6729_cov_4.258016"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKK95_10905"
     CDS             <1..371
                     /locus_tag="PKK95_10905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC18771.1"
                     /translation="RLKAFDIFVMSSVTEGLGTSLLDAMAAARPIVATRAGGIPEVVE
                     DGVSGLLVPPADGPALAAALLALLADQPRRRRMGEAGLARARQRFSADRMVSETVAAY
                     ERVLASRSHAADSASLPRGG"
     gene            complement(268..1212)
                     /locus_tag="PKK95_10910"
     CDS             complement(268..1212)
                     /locus_tag="PKK95_10910"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOC18772.1"
                     /translation="MRHPRQDVSATVRRAPGAAEGLGAALERLYRSYDRVDSATDPIQ
                     VVRRFAAPADREVVGFVASALAFGRVASVMQSIERVLEVLGPSPAAFVQGFRPARDGV
                     ALARVVHRWTRGPDLVALLLVLRGILTRSGSIEAFFGEGDSPSSPDVGPGLESFSRRA
                     LEVDLAPAYGRVPPRPGVCYFFPRPSSGSACKRLNLFLRWMVRRDGIDLGVWRSPAPS
                     RLIVPLDVHLVRMGRCLGLTRYRTPGWRMAAEITAALRRIDPDDPVRFDFALCHLSMT
                     GGCGLNRPSGDSHCPLRGFCSPGPARTRRPSRIPSDRR"
     gene            complement(1218..2552)
                     /locus_tag="PKK95_10915"
     CDS             complement(1218..2552)
                     /locus_tag="PKK95_10915"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOC18773.1"
                     /translation="MKRLLRGGRVVDPTQGLDAVADVLIEDGRIAGVGPMRAGAGVEV
                     VEVPAGCVVCPGFIDMHVHLREPGQEHKETIATGTAAAAAGGFTAVACMPNTEPANDC
                     ASLTEAILKAAAATAHVRVYPIGAVTKGMKGEQLAEIADLKAAGCVAVSDDGRPVPSA
                     IIMRRALEYTLMLGLPVIDHCEEPTLKGEGTAHEGYMASVLGLRGIPAAAESIMVERD
                     VALAEMTGGRVHIAHLSTRQALRSVRAGRERGVRVTCEVTPHHFTLTDARLADPIPYD
                     TNLKMNPPLREAADVDALLDGLRDGTVDAIATDHAPHQPDEKFVEFDRAPFGIIGLET
                     AVPLALDRLVRPGVIALPRLVELMSSNPARILGVAGGSLKAGSPADVTIIAPEAAFTV
                     DLARSRSKSRNSPFDGWRLAGAVAATIVAGRTVYVNAEVDGAAGLGPAAARA"
     gene            complement(2536..3519)
                     /locus_tag="PKK95_10920"
     CDS             complement(2536..3519)
                     /locus_tag="PKK95_10920"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOC18774.1"
                     /translation="MNPETPDIAAQPAGAPRLRRKDLVGIAELSSEEITLILDTAVAM
                     KEVSSRAIKKVPALRGKTVVNLFFEPSTRTRTSFEIAEKRLSADTLNIAASTSSVVKG
                     ETLADTVANIEAMAPDLLVVRHSSSGTCHFLSRICRAGVINAGDGMHEHPTQALLDAF
                     TMRERKGRLAGLKVAIVGDLLHSRVVRSNVQLLTKMGAEVWVCGPPTLVPAGFDRTGA
                     RVTYSVEEAVRDADVVMMLRIQLERMLGGFFPSLREYFEVFGMTVERVALARPDVLIM
                     HPGPMNRGVEIASDVADGPYSVILDQVSNGVAVRMAVLYLLAGGAEHETAA"
     gene            complement(3516..4067)
                     /gene="pyrR"
                     /locus_tag="PKK95_10925"
     CDS             complement(3516..4067)
                     /gene="pyrR"
                     /locus_tag="PKK95_10925"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOC18775.1"
                     /translation="MPVVMDADRMSRTLTRIAHEIVERNRGVEPVALVGVRTRGVPLA
                     RRLADRLQEITGQEVPTGALDITLYRDDLMHNPVGPQPLVRSTEIPFSIDNRTIILVD
                     DVLYTGRTVRAALDELIDFGRPRSIQLVVIVDRGHRELPIKADYVGKNLPTSRSESVS
                     VRLQEIDGRDEVVIEKIPEEQAL"
     gene            complement(4386..6140)
                     /locus_tag="PKK95_10930"
     CDS             complement(4386..6140)
                     /locus_tag="PKK95_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC18776.1"
                     /translation="MRYLRMFSNAAIAAALGAVYLTVLTLQLNPMVPLYPMNLAGLLL
                     TLGLYYGVHLAALFYGVIVFRQILSAEVFSPGWISLRVLSWLLVLDAGGAAALMWFNL
                     LAYAPMQSLDTARRMAAGAAVLTICALLFLGVALAHYTFGRRRGRVGGTVVALALLAS
                     LAVPLAARGPGSPLPLFSRPLDVDIGIERPAHGGRVVMVLLDGGSLDFVSTAALAGRL
                     PNFGRILDRGAAMHLNTHRPTQPEPVWTSVATGKLPWKSGVRSAGRYHVRLATDHLEL
                     LPENCFAQGLITYGLIGPEAHDAGSLRARPLWSILATAGLTTGVVNWPLTYPAQPVRG
                     YLVSDRFYRPGDAWLEPDDPAAIYPVDLLPVARLAGDRARRLFDASAVVEARAGAESG
                     LGGDEMLTDRMHEEIARGLQQQFRPHLSAVRFRQLDPAGHEYLRYAMPAAFGDVTPEE
                     RRRYGQVLELAYARVDAMVGRVMATLGAEDLLLVVSGYGMEPLPMWKRAVERAIGNPQ
                     LSGTHEYAPDGFLLAYGPSIASGRKRRGSVVDVAPTVLYFLGLPVGRDMDGYARTDIF
                     VRDFVETRPIAFIPSYER"
     gene            6211..>6729
                     /locus_tag="PKK95_10935"
     CDS             6211..>6729
                     /locus_tag="PKK95_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18777.1"
                     /translation="MPVDVEAVVLSNTRLPGGYSVLELRAPELASLAEPGQFVMVRAS
                     HCDAPLLRRPYSIFDLVRDADGRASGFTLLNKRVGVGSGLLYEAAPGARFCCLGPLGR
                     PFTLAAPPVEAWMVAGGVGLAPFATLAPALAGRGVAATLFYGGRTSADIFCLDRFEPH
                     GVVTGHPNIKFAT"
BASE COUNT          976 a         2375 c         2464 g          914 t
ORIGIN      
        1 cgcgcctgaa ggcgttcgac atcttcgtga tgagctcggt gaccgagggg ctcggcacct
       61 cgctgctcga tgcgatggcc gccgcgcggc cgatcgtcgc cacgcgcgcg ggcggaatcc
      121 ccgaggtggt cgaggacggg gtgagcggcc tcctcgtacc gcccgcggac gggcccgccc
      181 tcgccgccgc gctcctggcg ctgctggccg accaaccgcg gcggcgccgc atgggagagg
      241 cgggcctggc ccgcgcacgg cagcgcttca gcgccgatcg gatggtatcc gagacggtcg
      301 ccgcgtacga gcgggtcctg gcgagcagaa gccacgcagc ggacagtgcg agtctcccga
      361 ggggcggttg aggccgcagc ctccggtcat gctcaggtgg cagagggcga agtcgaaccg
      421 gaccggatcg tccgggtcga tgcggcgcag cgcggcggtg atttccgccg ccatccgcca
      481 ccccggggtg cggtagcgtg tcagccccag gcaccgcccc atccgcacga ggtgcacgtc
      541 gagcggaacg atcagtcgcg aaggggccgg cgaccgccag accccgaggt cgatcccgtc
      601 gcggcgcacc atccaccgca ggaacaagtt caaccgcttg caggcgctgc cgctcgaagg
      661 gcgcgggaag aaatagcaga cacccgggcg cggcgggacg cgcccgtatg ccggggccag
      721 gtccacctcg agcgcccggc gcgagaacga ctcgaggccc gggccgacgt cgggggagga
      781 cggggaatcg ccctcgccga agaacgcctc gatcgaccct gaccgcgtga ggatgccgcg
      841 caaaacgagg aggagggcga ccaggtcggg accgcgcgtc caccggtgaa cgacgcgggc
      901 gagcgccacg ccgtcccgtg cggggcggaa gccttgaacg aacgcggccg gcgaggggcc
      961 gagcacctcg aggacgcgct cgatcgactg catcacgctg gcgacgcggc cgaacgccaa
     1021 ggccgaggcc acgaacccga ccacctcgcg gtccgcaggc gccgcgaagc ggcggaccac
     1081 ctggatcgga tcggtcgccg agtcgacgcg gtcgtacgac ctgtagagcc gctcgagggc
     1141 ggcgccgagt ccctcggccg cccccggcgc ccgccggacc gtcgccgaca cgtcctggcg
     1201 cgggtggcgc accgcttcta ggcccgtgcc gcggccggtc ccagccccgc cgcgccgtcc
     1261 acctccgcgt tcacgtacac ggtccggccc gcgacgatcg tcgcggccac ggccccggcg
     1321 agtctccacc cgtcgaatgg ggagttgcgc gacttggatc gcgaccgggc gaggtcgacg
     1381 gtgaaggccg cttccggcgc gatgatcgtc acgtcggccg gcgaacccgc tttcaggctg
     1441 cccccggcca ccccgaggat ccgggccggg ttcgacgaca tcagctccac gagccgcggg
     1501 agggcgatga cgcccgggcg cacgagccga tcgagggcga gcgggacggc ggtctcgagg
     1561 ccgatgatcc cgaacggcgc gcggtcgaac tcgacgaact tctcgtcggg ctggtgcggc
     1621 gcgtgatcgg tggcaatcgc gtccaccgtc ccgtcgcgca gcccgtcgag gagggcgtcc
     1681 acgtcggccg cctcgcgcag cggggggttc atcttgaggt tggtgtcgta ggggatggga
     1741 tcggccagcc gggcgtcggt gagcgtgaag tggtgcggcg tcacctcaca ggtgacccgc
     1801 acgccgcgct cgcggccggc gcgcacggat cgaagggcct gccgggtgct gaggtgcgcg
     1861 atgtgcacgc ggccgccggt catctcggcc agcgcgacgt cgcgctccac catgatcgac
     1921 tcagccgcgg ccgggatgcc gcgcaggccc agcaccgagg ccatgtaccc ctcgtgtgcc
     1981 gtcccctcgc ccttgagcgt cggttcctcg cagtggtcga tcaccggcag gccgagcatc
     2041 aaggtgtact cgagggcccg cctcatgatg atcgcgctcg gcaccggccg gccgtcgtcg
     2101 gagacggcga cgcagccagc ggccttgagg tcggcgatct cggcgagctg ctcgcccttc
     2161 atccctttgg tcaccgcgcc gatcgggtac acgcgcacgt gtgcggttgc cgcggccgcc
     2221 ttgaggatcg cctcggtgag gctggcgcag tcgttcgccg gctcggtgtt cggcatgcag
     2281 gcgaccgcgg tgaagccgcc cgcggcggct gcggcggtcc cggtggcgat cgtctccttg
     2341 tgctcctggc cgggctcgcg caggtggacg tgcatgtcga tgaagcccgg gcagacgacg
     2401 cagccggccg ggacctctac cacctcgacg ccggcgccag cccgcatcgg cccgaccccg
     2461 gcgatccgcc cgtcttcgat caggacgtcg gcaaccgcgt ctagcccctg cgtgggatcg
     2521 acgacgcgcc cgcccctaag cagccgtttc atgttcagct ccgccagcga ggaggtacag
     2581 caccgccatc cggacggcga cgccgttgga tacctggtcg aggatcaccg agtagggccc
     2641 atcggcgacg tcggacgcga tctcgacgcc gcggttcatc gggccggggt gcatgatgag
     2701 cacgtcgggc ctggcgagcg ccacgcgctc gacggtcatc ccgaagacct cgaagtactc
     2761 gcgaagggac gggaagaagc cgccgagcat ccgctcgagc tggatgcgaa gcatcatcac
     2821 gacgtccgcg tcgcgcaccg cctcctcgac cgagtaggtg acgcgcgcgc cggtacggtc
     2881 gaagccggcg ggcaccagcg tcggcgggcc gcacacccag acctcggcgc ccatcttggt
     2941 cagcagctgc acgttcgagc ggacgacccg gctgtggagc aggtcgccca cgatggcgac
     3001 cttcagcccg gcgaggcggc ccttgcgctc gcgcatcgtg aaggcgtcga gcagcgcctg
     3061 cgtcgggtgc tcgtgcatgc cgtcgccggc gttgatgacg cccgcgcggc agatccggga
     3121 gaggaagtgg caggtgcccg aggaggagtg gcgcaccacc agcaggtcgg gcgccatcgc
     3181 ctcgatgttc gccaccgtgt cggccagcgt ctcccccttg acgacgctcg aggtactcgc
     3241 cgcgatgttc agcgtgtcgg cgctcaggcg cttctcggcg atctcgaacg aggtgcgggt
     3301 gcgggtgctc ggctcgaaga acaggttgac gaccgtcttg ccccgcagcg ccggcacctt
     3361 cttgatggcg cgcgaggaca cctccttcat cgccaccgcc gtgtcgagga tcagggtgat
     3421 ctcctcggac gacagctcgg cgatgccgac caggtccttg cgccgcaggc ggggcgctcc
     3481 ggcgggctgg gccgcgatgt ccggggtctc tgggttcata gcgcttgttc ttccgggatc
     3541 ttctcgatga ccacctcgtc gcgcccgtcg atctcctgca gccggacgga cacgctctcg
     3601 cttctcgagg tcggcaggtt cttccccacg tagtcggcct tgatcggcag ctcgcggtgt
     3661 ccgcggtcga cgatcaccac cagctgaatc gagcgcgggc gcccgaaatc gatcagctcg
     3721 tcgagcgccg cccgcaccgt ccgccccgtg tagagcacgt cgtccacgag gatgatcgtg
     3781 cggttgtcga tcgagaacgg gatctcggtg ctgcgaacca gcggctgcgg gccgaccggg
     3841 ttgtgcatca ggtcgtcgcg gtacagggtg atgtcgagcg cgccggtcgg cacttcctgg
     3901 cctgtgattt cctggaggcg gtcggccagg cgccgcgcca gcggtactcc ccgcgtccgg
     3961 acgcccacga gcgccaccgg ctccacgccc cggttgcgct ccacgatctc gtgcgcgatc
     4021 cgggtgaggg tgcggctcat ccggtccgcg tccatcacga caggcatggc aatcctcggg
     4081 ttgaaggccg acgggcgccc ggccgggcgg cagacacggc gggccgggtt ggcgcggacg
     4141 gctgaagatg aagaggctgg atgcggccca tgggtcttgc cgacctcacc gggccggctt
     4201 aaagaacgcc gaagtatacc acgcggggtg aggccggccg gcccggcggg gaccccggtt
     4261 tgaagcatag agatcacgaa gatcacgagg aaatgcccgt gaacgcgccg gccttcggcg
     4321 gcggcgaggc ggccgggtac gagggccgga agcgccgtga ggggcgaccg cccgaagggc
     4381 ggccgtcagc gctcgtagct cgggatgaag gcgatcgggc gggtctcgac gaagtcgcgc
     4441 acgaagatgt cggtgcgcgc gtagccgtcc atgtcgcggc caaccggcag gccgaggaag
     4501 tacaggaccg tcggcgcgac gtccaccacc gagccgcgcc gcttccgtcc cgacgcgatc
     4561 gacgggccgt acgcgaggag gaagccgtcg ggcgcgtact catgggtgcc cgacagctgg
     4621 gggttgccga tcgcccgctc gacggcccgc ttccacatcg ggagcggttc catgccgtag
     4681 cccgatacca cgagcagcag atcctcggcg ccgagcgttg ccattacccg gcccaccatc
     4741 gcgtccaccc gcgcgtacgc cagctcgagc acctggccgt accggcgccg ctcctcgggt
     4801 gtcacgtcgc cgaaggcggc cggcatcgcg tagcgcaggt actcgtggcc ggccggatcg
     4861 agctggcgga agcgcaccgc cgacaggtgc gggcggaact gctgctgcag gccccgcgcg
     4921 atctcctcgt gcatccggtc ggtcagcatc tcgtcgccgc ccagcccgct ctcggcgcca
     4981 gcccgcgcct cgacgaccgc ggaggcgtcg aacaggcgcc gggctcgatc accggccaga
     5041 cgcgccaccg gcaggaggtc caccgggtag atggccgccg gatcgtcggg ctcgagccag
     5101 gcatcgccgg gccgatagaa ccggtcgctc accaggtatc cgcggaccgg ttgcgcgggg
     5161 taggtgagcg gccagttgac gacgccggtg gtgaggcccg cggtggcgag gatgctccag
     5221 agggggcgcg cgcggagcga gcccgcgtcg tgcgcctcgg gcccgatcag cccgtaggtg
     5281 atcaggcctt gcgcgaagca gttctcgggg agcagctcga ggtggtcggt ggccagcctg
     5341 acgtggtagc gcccggccga gcgcacgccg ctcttccacg gcagcttgcc cgtggccacc
     5401 gacgtccaca ccggctcggg ctgtgtcggc cggtgcgtgt tgaggtgcat ggccgcgccc
     5461 cggtcgagga tccgcccgaa attgggcagg cgcccggcga gggcggcggt ggagacgaag
     5521 tcgagcgagc cgccgtcgag cagcaccatc accacgcggc cgccgtgggc ggggcgctcg
     5581 atcccgatgt cgacgtcgag gggccgcgag aagagcggga gagggctgcc cggcccgcgc
     5641 gccgccagcg ggacggcgag ggacgccagc agcgcgaggg ccacgacggt cccgccgacg
     5701 cgaccccggc ggcggccgaa cgtgtagtgc gcgagcgcga cgccgaggaa caggagcgcg
     5761 cagattgtca ggaccgccgc acccgccgcc atccggcgcg cggtgtcgag gctctgcatc
     5821 ggcgcgtagg ccagcaggtt gaaccacatc agggccgcgg cccctcccgc gtcgagcacg
     5881 agcagccatg agagcacgcg caggctgatc caccccggtg agaagacctc cgccgacagg
     5941 atctggcgga acacgatcac cccgtagaag agcgccgcga gatgcacccc gtagtacagg
     6001 ccgagcgtga ggaggagccc cgccaggttc atcgggtaga gcgggaccat cgggttgagc
     6061 tgcagggtga gcaccgtgag gtacacggca cccagcgccg cggcaatcgc cgcgttgctg
     6121 aacatccgga ggtaacgcat ccgcgcagga tgttagcgca tcgggcgccc tcccgggcgg
     6181 gtcgtgaagc tccggggtat actcggggcc atgcccgtcg acgtcgaggc cgtggtcctc
     6241 tccaacactc gcctgccggg cgggtactcg gtgctcgagc tgcgcgcgcc ggagctggcg
     6301 tccctcgccg agcccggcca gttcgtcatg gtgcgcgcct cgcactgcga cgctcccctg
     6361 ctgcggcgcc cctactcgat cttcgacctc gtgcgggacg cggacgggcg cgcttcgggc
     6421 ttcaccctgt tgaacaagcg agtgggggtc gggtcgggtc tcctctacga ggcggcgccc
     6481 ggcgcacgct tctgctgcct cggtccgctc gggcggccgt tcacgctggc ggccccgcca
     6541 gtcgaggcgt ggatggtggc cggcggcgtc ggcctcgccc cctttgccac gctcgccccg
     6601 gcgctcgccg gccgtggggt ggcggcgacg ctgttctacg gcgggcgaac ctctgccgac
     6661 atcttctgcc tcgaccgctt cgagccgcac ggcgtggtca ccgggcaccc gaacatcaag
     6721 ttcgcgacg
//
LOCUS       DAOPQX010000196         6723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12511_length_6723_cov_6.653419, whole genome shotgun sequence.
ACCESSION   DAOPQX010000196 DAOPQX010000000
VERSION     DAOPQX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12511_length_6723_cov_6.653419"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PKK95_10940"
     CDS             <1..779
                     /locus_tag="PKK95_10940"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOC18778.1"
                     /translation="PDEFVGTWAIQGMWDESISPSELGWGTHERELPPHALLPAEGPR
                     NQIILPRMGLNTQVRSWVPGQDIVGMVVTHGESFGVSHALTVHENGVPVYRPTVHYAY
                     LPSNDSQVSLHELRCRNYELHPRQRIMGDEITAGMDLFGALLMGHPFDSWWTGSILSI
                     AQARRCVPHVNATALQVAAGVLAAVLWVERNPRRGFHLPENLPHEEILRDARPYLGRV
                     VSTRSDWTPLKRHRVYFDENPEARADHEDPWQFRNFLFTP"
     gene            complement(1057..1350)
                     /locus_tag="PKK95_10945"
     CDS             complement(1057..1350)
                     /locus_tag="PKK95_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HOC18779.1"
                     /translation="MRQARREGKETPYHFIEVMACPGGCIGGGGQPYGVDNERRRQRI
                     AGLYQDDRDQAVRCSHENPEVVRLYRDFLGQPNGTKAHELLHTRYTARPLYKR"
     gene            complement(1415..3229)
                     /locus_tag="PKK95_10950"
     CDS             complement(1415..3229)
                     /locus_tag="PKK95_10950"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOC18780.1"
                     /translation="MFLRRKDKHVRGVGYSYWNLCVTYRTARGPRQRVVASLGKLEER
                     EVPGLRGGWDDLPALLRGEPAAKVPRQVPLPGFGPAAEAPAQWEEVDVRGLRIERARD
                     FGEPYLALALWHRLGLDRLMAELLPAGRESVEWSQVAALLTVARFCAQRSELGVAEHW
                     YDTTALDDLLGVGSGVINDARLYRALDHLGAHKDRLCSHLMERYRQWFGVRFEFLLYD
                     VTSTYFEGQAERNPQAQRGYSRDHRSDCKQVCIGLVCTPEGLPLSFEVFAGNRTDVTT
                     VEHIVRTMEQKYGQAARIWVMDRGMVSEANLEFLRARKARYLVGTPKRWLRAHEAELL
                     EHSEWTEVREDLEVRLVEHPGGVPGERFVLCRSSARALKERAMLARQTERLTTELAKI
                     DTALRRTPQSDLEAVGRRIGRHLGRYPAAAAILHAQVEHDGQGRACALHVSSDLAAGQ
                     KAHREKGAYLLRTNCEETDPAQLWRWYVQLTQAESAFRTAKSDLGLRPVFHHKEDRVQ
                     AHILVCFLSLALWRSLEQWMSAQGLGTCARQLVKQLATIKSMDVVLPVRRTGVVTQLR
                     LRVVATPEPATAQLLAHLGLRLPKGTRTISDVVPKSAA"
     gene            complement(3282..4856)
                     /locus_tag="PKK95_10955"
     CDS             complement(3282..4856)
                     /locus_tag="PKK95_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOC18781.1"
                     /translation="MSAVATLPAVKATINGLMVEVPAGTSILDAARRAAVKIPTLCKH
                     KDLLPTAACGLCVVRNKGGPRLLRACCTPLEDGMEIVTHDNEIVEVRRSTLELILSNH
                     PAECLTCGRNGECELQAIAADFNIRTDCIKRYVKDLPADRSTGSIILEFDKCIKCGRC
                     LQVCQEMQDVWALSFLERGINTRMAPAGDIALAESPCVMCGQCSAHCPTGAIVENDET
                     AAVWAALNDHDKVCVVQIAPSVRVTVGEAFGLPPGTNLTKKLYSALRRLGFHAVFDTN
                     FSADVTIVEEATEFVQRFVHQRGPLPLITSCCPAWTDFMEKKHWDFIDNFSTAKSPQQ
                     MLGVLAKTYYAQQAGIDPAKLVVVSIMPCTAKKFELSRTEEMFASGFKDVDICLTTRE
                     LARMLKQSGIDFLALDDGAADSILGQYSGAGTIFGTTGGVMEAALRTAHHYATGQKLG
                     QVEFAAVRGLDGVRTAVVDVGGAKVRVAVAHGLGHVSTVLARVRQFGVRRRKVSEESG
                     FGRSRRPEGARRREGL"
     gene            complement(4859..>6723)
                     /locus_tag="PKK95_10960"
     CDS             complement(4859..>6723)
                     /locus_tag="PKK95_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOC18782.1"
                     /translation="AGTLTATVDVVQIQQGSGNTIINESTGTISSALSVAAAGLPPVL
                     YGGVKADVAPQIVAAHLQSGRLIDDHVIAPAGGAAELASPVTHLLVLDTGVGEAKTKF
                     FQFSFVEELKRRGFADQVQVVRALDLGVYDEGVVVTLLPAGVTYTNVLAPDVARIVEQ
                     SVQGRQVVDDLRWTKPDPQVRIVLRNCGSIDPESLQDYLERAHGYQGLRAALSRPPEQ
                     VIEELKRSGLRGRGGAGFPTWQKWSFTRQQKAEPRCVICNGDEGDPGAFMDRSVLESD
                     PHNVLEGLMIAGYAIGAAHGYFYIRAEYPKAVERVERALVQAREAGLLGRNILGTDFS
                     FDAKIRLGAGAFVCGEETALIASIEGKRGSPSPRPPYPSVRGLWGRPTAINNVETLAN
                     VPAILLRGGAWYAGYGTETTKGTKVFAVTGKVKTAQLVEVPMGTTLRSIVFDVCGGVL
                     EDRDIKAVQTGGPSGGVIPDKHLDTPVAYETLQQLGSIMGSGGMIVMDTTDSMVDVAK
                     FFLKFCVDESCGKCAPCRIGGFQMLQILDRISRGRGAAEDIAALRRICQAMQKASLCG
                     LGQTAPNPVLSTLRYFDEEYKAYIEGGPSYARKMARAAGGAEPARAVATAAEGA"
BASE COUNT         1023 a         2349 c         2254 g         1097 t
ORIGIN      
        1 gccccgacga gttcgtcggc acctgggcga tccagggcat gtgggacgag tcgatctcgc
       61 cgagcgagct cggctggggc acacacgagc gcgaactgcc accccacgcc ctgctgcccg
      121 ccgagggccc gcgtaaccag atcatcctgc cgcgcatggg cctcaacacc caggtccgct
      181 cctgggtccc cgggcaggac atcgtcggaa tggtcgtcac ccacggcgaa tcgttcgggg
      241 tgtcgcacgc gctcaccgtg catgagaacg gcgtgcccgt ctaccgcccg acggtccact
      301 atgcgtacct gccgtccaac gactcacagg tctccctcca cgaactgcgg tgccgcaact
      361 acgagctgca cccccgccag cggatcatgg gcgacgagat caccgccggg atggacttgt
      421 tcggcgcgct cctcatgggg cacccgttcg actcctggtg gacgggcagc attctctcca
      481 tcgcgcaggc ccgccgctgc gtcccgcatg tgaacgccac cgcgttacag gtcgccgccg
      541 gcgtcctcgc ggccgtgctc tgggtggaac gtaatccccg ccgcggcttc caccttcccg
      601 agaacctgcc gcacgaggag atcctgcgcg acgcccggcc gtacctcggc cgcgtcgtct
      661 ccacccgttc ggattggacg ccgctcaagc gccaccgcgt ctacttcgac gagaaccccg
      721 aggcgcgcgc cgaccacgag gatccgtggc agttccgcaa cttcctgttc acaccctgag
      781 gtgtggcgcg caggagcggc agccacgtgg cggggtgctt cagcccccgc cgctcgactc
      841 cgcccaccgc aagcgtcgcc tctccgcagg cgtcgcctct ccgcaggcat cgtccgccgc
      901 aggcgcccct tctccgcccc acgcttcgcc aacgccagca tcgccctctc gtatccgccg
      961 gcccgggcta aagcaccggg ccacgtggcg gggtgcttca gcccccgccg ttcgactccg
     1021 cccaccgcag gcgcctcacc gccgccggct acagcctcac cgcttgtaga gcgggcgggc
     1081 ggtgtagcgg gtgtggagca actcgtgcgc ctttgtgccg ttgggctggc caaggaagtc
     1141 ccgatacagg cgcaccacct ccgggttctc atgcgagcag cggacggcct ggtcgcggtc
     1201 gtcctggtag aggccggcga tgcgttgccg ccgcctctcg ttgtcgacgc cgtagggctg
     1261 gccgccgccg ccgatgcagc cgccggggca cgccatcacc tcgatgaagt ggtacggcgt
     1321 ctccttgccc tcgcggcgcg cctgtcgcac ctgttctaac ccaagttcgt cagttcaggg
     1381 aacgactttt cgagtcatca acgagttgcg tgattcaagc cgcggatttc ggcactacat
     1441 cgctgatggt gcgggtgcct ttgggaaggc gtaggccgag atgggcgagc agttgggcgg
     1501 tggccggctc gggggtggcg acgacacgca gccgcagttg cgtcaccaca ccggtgcggc
     1561 ggaccggcag caccacgtcc atgcttttga tggtggcgag ctgtttgacc agttgacggg
     1621 cgcaagtacc caggccttgg gcactcatcc attgctcgag gctgcgccac agcgccaacg
     1681 agaggaagca gaccagtata tgcgcctgca cgcggtcttc cttgtggtgg aagaccgggc
     1741 gcagtcccag gtcgctcttg gcggtgcgga acgcgctttc ggcctgggtg agctgcacgt
     1801 accaacgcca cagttgcgcg gggtcggtct cctcgcagtt ggtacgcaac aggtaggcgc
     1861 ctttctcgcg gtgggctttc tggccggcgg ccagatcgct gctcacgtgc aacgcgcagg
     1921 cgcggccctg cccgtcgtgt tcgacctgcg cgtgcaggat cgccgcggcg gcgggatagc
     1981 ggccgagatg acgcccgatg cgtcggccca ccgcctccag atccgactgc ggcgtgcgtc
     2041 gcagcgccgt gtcgatcttg gccagctcgg tggtgagccg ctcggtctgc cgggcgagca
     2101 tcgcgcgctc tttcagggcg cgggcactgc tgcgacacaa cacgaagcgt tcgcccggta
     2161 cgccgccggg atgctccacc agacgcactt cgaggtcctc gcgcacctcg gtccattcgg
     2221 agtgttcgag cagttcggcc tcgtgtgcgc gtaaccaacg tttcggtgtg ccgactagat
     2281 agcgggcctt gcgcgcgcgc aggaactcca ggttggcctc gctgaccatg ccgcggtcca
     2341 tcacccagat gcgcgcggcc tgcccgtact tttgttccat cgtgcgcacg atgtgctcga
     2401 cggttgtcac atccgtgcgg ttgcccgcga agacctcgaa ggacaatggc aacccctcgg
     2461 gcgtacacac cagtccgatg cacacctgct tgcagtcgct gcgatgatcg cgcgagtagc
     2521 cgcgctgcgc ctgcgggttg cgctcggcct gcccctcgaa gtaggtgctc gtcacgtcgt
     2581 agagcaggaa ctcgaagcgc acgccgaacc actgccggta gcgctccatc aggtgcgagc
     2641 acagccggtc cttgtgggca ccgagatgat cgagcgcgcg gtacagccgc gcgtcgttga
     2701 tcacccccga gcccacgccg agcagatcgt cgagcgcggt cgtgtcgtac caatgctcgg
     2761 ccacgcccag ctcggagcgt tgcgcgcaaa accgcgccac cgtcagcaac gccgccacct
     2821 gcgaccattc gacggattcg cgaccggcgg gcaacagctc cgccatcaac cggtccagcc
     2881 cgagccggtg ccacaacgcc aacgccaagt acggctcgcc gaagtcgcgc gcccgctcga
     2941 tgcgcaaccc ccgcacgtcg acctcctccc attgcgccgg cgcctcggct gccggcccga
     3001 agccgggcag cggcacctgc cgcggcacct tcgccgccgg ctccccacgc aacaaggccg
     3061 gcagatcatc ccagccgccc cgcagtcccg gaacctcgcg ctcctccagt ttgcccagcg
     3121 acgccaccac ccgctgccgc ggtccccgcg ccgtgcggta ggttacgcac aggttccagt
     3181 agctgtaacc gacaccccgc acgtgcttgt cctttcgtcg caggaacatg gtggcgggat
     3241 tttcgcagat cccgcgcacc ccgccaaggg ggtgttcggc actacaagcc ctccctgcgc
     3301 ctcgctccct ctgggcgcct cgaccgacca aacccgctct cctcggaaac ttttctccgc
     3361 cttaccccaa actgacgaac tcgggctaac acggtcgaca catggccgag gccgtgcgcc
     3421 acagcgacac ggaccttggc tccgccgacg tcgaccactg cggtccgcac gccgtcgagt
     3481 ccgcggaccg cggcgaactc cacctgcccg agcttctgcc cggtagcgta gtggtgggcg
     3541 gtgcgcaacg cggcctccat cacgccgccg gtggtgccga agatcgtgcc ggcgccactg
     3601 tactgcccga ggatcgagtc ggccgcaccg tcgtcgagcg cgaggaaatc gatgcccgac
     3661 tgcttgagca tgcgggcgag ttcgcgcgtg gtcaggcaga tgtccacgtc cttgaagccg
     3721 gaggcgaaca tctcctcggt gcgcgacagc tcgaacttct tcgcggtgca gggcatgatc
     3781 gagaccacga cgagcttcgc gggatcgatc ccggcctgtt gcgcgtagta ggtcttggcg
     3841 agcacgccga gcatctgctg gggggacttg gcggtggaga agttgtcgat gaagtcccag
     3901 tgcttcttct ccatgaagtc ggtccaggcg gggcagcacg aggtgatgag cggcagcggc
     3961 ccgcgctggt ggacgaagcg ttggacgaac tcggtggcct cctcgacgat ggtgacgtcg
     4021 gccgagaagt tcgtgtcgaa cacggcgtgg aacccgagcc ggcgcagggc ggagtagagc
     4081 ttcttcgtga ggttcgtgcc cggcggcagg ccgaacgcct cgcccaccgt cacacgcacg
     4141 ctcggcgcga tctgaaccac gcagaccttg tcatggtcgt tcaacgccgc ccagaccgcg
     4201 gcggtctcgt cgttctcgac gatggcaccg gtcgggcagt gggcggagca ctggccgcac
     4261 atcacgcagg gcgactcggc cagcgcgatg tcgccggcgg gggccatgcg ggtgttgatg
     4321 ccgcgttcga ggaacgagag cgcccacacg tcctgcatct cctggcacac ctggaggcag
     4381 cggccgcatt tgatgcactt gtcgaactcg aggatgatcg agccggtgga gcggtcggca
     4441 ggcaggtcct tgacgtagcg tttgatgcag tcggtgcgga tgttgaagtc ggcggcgatc
     4501 gcctggagtt cgcactcgcc gttgcggccg caggtgaggc actcggccgg gtggttggag
     4561 aggataagct cgagcgtgga gcggcggact tcgacgatct cgttgtcgtg ggtgacgatc
     4621 tccatgccgt cctccagcgg ggtgcagcag gcgcgcagga ggcgcgggcc gcccttgttg
     4681 cgcacgacgc acaggccgca ggccgcggtg ggcaggaggt ccttgtgctt gcacagcgtg
     4741 gggatcttca cggcggcgcg ccgggcggcg tcgaggatcg aggtaccggc cggcacctcg
     4801 accatgagcc cgttgatggt ggccttgacg gcgggaagtg tggcgacggc gctcatggtc
     4861 aggctccttc ggcggcggtg gcgacggccc gggcgggttc ggcgccgccg gcggcgcggg
     4921 ccatcttgcg agcgtaactg ggaccgccct cgatgtacgc tttgtactcc tcgtcgaagt
     4981 agcgcagcgt cgagagcacg gggttcggcg cggtctggcc gaggccgcaa agcgaggcct
     5041 tctgcatcgc ctggcagatg cggcgcagcg ccgcgatgtc ctcggcggcg ccccggccgc
     5101 gggagatgcg gtcgaggatc tgcaacatct ggaagccgcc gatccggcac ggcgcgcact
     5161 taccgcacga ctcgtcgacg cagaacttca ggaagaactt cgcgacgtcg accatcgagt
     5221 ccgtcgtatc catcacgatc atgccgcccg agcccatgat ggagccgagc tgctggaggg
     5281 tctcgtaggc cacgggcgtg tcgaggtgct tgtcggggat gacgccgccc gacgggccgc
     5341 cggtctgcac ggccttgatg tccctgtcct cgagcacgcc gccgcacacg tcgaacacga
     5401 tggagcgcag cgtggtgccc atcggcacct cgacgagctg ggcggtcttc accttgccgg
     5461 tgacggcgaa gaccttggtg cccttcgtcg tctcggtgcc gtagccggcg taccaggcgc
     5521 cgccgcgcaa caggatggcc gggacgttgg cgagggtctc gacgttgttg atcgcggtgg
     5581 gccggcccca caggccgcgg accgagggat acggcgggcg cgggctgggg ctgccgcgtt
     5641 tgccctcgat ggaggcgatg agggccgtct cctcgccgca aacaaacgcg ccggcgccga
     5701 gccggatctt ggcgtcgaac gagaagtccg tgccgaggat gttgcgcccg agcagtccgg
     5761 cctcgcgggc ctgaaccagc gcccgctcga cacgctcgac cgccttcggg tactcggccc
     5821 ggatgtagaa atagccgtgg gccgcgccga tcgcgtagcc ggcgatcatc agcccctcga
     5881 gcacgttgtg cggatcgctc tcgagcacgc tgcggtccat gaatgcgccg gggtcgccct
     5941 cgtcgccgtt gcagatcacg caccgcggct ccgctttttg ctgacgcgtg aagctccact
     6001 tctgccaagt ggggaacccg gcgccgccac gcccgcgcag cccgctgcgc ttcagctcct
     6061 cgatcacctg ctcgggcggt cgcgacaacg ccgcccggag cccctggtat ccgtgcgcgc
     6121 gttccagata gtcctgcaac gactcggggt cgatcgaccc gcagttgcgc aggacgatcc
     6181 ggacctgcgg atcgggcttc gtccagcgca ggtcgtcgac cacctgccgg ccctggaccg
     6241 actgctcgac gatgcgcgcc acgtcgggtg cgagcacatt ggtgtaggtc acgcctgcgg
     6301 gcaggagggt caccaccacg ccctcgtcgt acacgccgag atcgagggca cgcaccacct
     6361 gcacctgatc ggcgaacccg cggcgcttca actcctcgac gaaggagaac tggaaaaact
     6421 tcgtcttcgc ctcgcccacg ccggtgtcga gcacgagcag gtgcgtgacc ggcgacgcga
     6481 gttccgccgc cccgccggcg ggagcgatca catggtcgtc gatgaggcgg ccggactgaa
     6541 ggtgcgcggc gacgatctgc ggcgccacgt ccgccttcac gccgccgtag agcactggcg
     6601 gcagaccggc ggcggcgacc gacagggccg acgagatcgt gcccgtgctc tcgttgatga
     6661 tagtattacc gctgccctgt tggatctgga ccacgtcgac ggtcgccgtg agcgtgccgg
     6721 cat
//
LOCUS       DAOPQX010000197         6700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12574_length_6700_cov_4.964485, whole genome shotgun sequence.
ACCESSION   DAOPQX010000197 DAOPQX010000000
VERSION     DAOPQX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12574_length_6700_cov_4.964485"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..2804)
                     /locus_tag="PKK95_10965"
     CDS             complement(312..2804)
                     /locus_tag="PKK95_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18783.1"
                     /translation="MSFLVTDLKHALRMAGKTPGVTLVAVLTLGLGIGAVTSIFSLAN
                     GVLLRPLPFREPDRLVAISEGLPRLDYPVVPFSPPDFDAYSRWNRTMEGVAAYDNRQV
                     ELSGVAQPERLVAARVTPNLFPLLGIEPALGRVFASGEAGEGRHVVILSDALWRRTFA
                     GDRRLLGRTIVIDRTPHEVVGVLGPRLEFPPRGEGATWAAFNNQPADLFLPKWFTPDD
                     LQGYGNQFNSSVIGRLRPGVTIEQARAEAEGLVPRLEEAYPAGVRTEPKFKLQLSVSP
                     LKQAIVGGVRTLILVLFGGVVLLLLVGCANVANLLLARGATRQREFALRAAMGAGRRR
                     LVAQLLTEGALLGLAGGVVGVLLAMWGTDVLVAALPVNIPRVEAVQVDTSVLAFALAA
                     SLATTLIFGLAPAIPFARSNRGEALREGGRGATTGPRTSRLLGGLVAVQFALALVLAI
                     GGGLLARSFERLLATDPGFSPTQVLSLSTALPASSYPEARQVRTFYDELVSRLARLPG
                     VEAAGGGTSLPMNTTETRMFSVERPAKGANRVSGSNTLVYVNGNYFPALGIRFVTGRA
                     FTEAEMKPDAPPVVIVNETLARLFFDGGRAVGERIKWGGAQSDAAWMTIVGVVADVKQ
                     AGLDSAATPSVYRPFAQASDAEIAFFRFMDVALKVRGIDPLSLVPAVRREVRAMDASL
                     PIANVTTMERVVTGTVRSQRFYTVLIAAFAGSALLLAAIGLFGVLATAVAQRTQEIGV
                     RVALGASPRSVFRLVLQNGLVYAGLGLIGGVAAAAAATRVLSGFLYGVSASDPLTFAA
                     GTLVLALVALGAGYLPARRATKIDPIAALRAE"
     gene            2978..4288
                     /locus_tag="PKK95_10970"
     CDS             2978..4288
                     /locus_tag="PKK95_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18784.1"
                     /translation="MPNDWLVEERCPQCGGPVSMHEADRILTCAYCKVRLYVLAGGAL
                     AKYCLPVKPAPIGTIVMVPYWRLRAHRYRLLPSGVVPGAADCTALAADVPGLASSLGV
                     RAQAVPLRFATPAAEGRFLPATRPPDLVFQQLERLRRVEQGLASGPRASEEREAAPFE
                     ATITTASSLVYTPVRVSARSVFDAVLNRRVAGIDADRWNDAVPGADDRPAPVRFLATL
                     CPECGWQLEGATGTLILLCVNCGRGWRAGKSQLVPADFDVLPADGWTPDAHLPFWRAE
                     ARAAATWADFVRLVNLPRVVQAAWEREPLGFWFPAFAAHPDQFLRAAQTLTCARPGRR
                     EAAATEQASTPAEQAPASLQPRKCRAVTLPEAALDEALFVLLASLGHARRGMREILAG
                     IDLHVHERRLVYVPARFNGRELVNPELGLTVQRSLRSSSFSSQL"
     gene            complement(4272..5279)
                     /locus_tag="PKK95_10975"
     CDS             complement(4272..5279)
                     /locus_tag="PKK95_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC18785.1"
                     /translation="MQKKRLGRTGLEITRVGFGAWAVGGGGYAFGWGPQDDNESIAAI
                     EKAADLGVNWIDTAPVYGLGRSEEVVGRALERIPAGMRPYVFTKCSLVWPEGGREVTH
                     SLDASSIRRECEASLRRLGVEAIDLYQVHWPRWTNAPDAAADRLEEGWGALAELKREG
                     KVRHIGVSNFGVDDLERANRVAPVESLQPPYSLLRRKIEAEVLPYCLAHDIGVIVYSP
                     MQAGLLSGKMTRERIAALPEDDWRKRTREYQEPNLSANLAFVEVLRTVAARHGRTPGE
                     AAIAWTLRHPAVTAAIAGFRRPAQVAEIVKAADFQLPDTDAAEIERAIPGADVSLKAG
                     S"
     gene            complement(5406..5642)
                     /locus_tag="PKK95_10980"
     CDS             complement(5406..5642)
                     /locus_tag="PKK95_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18786.1"
                     /translation="MQGVLKVQVQEVLKVLMVPEELVPRVPRPHPLHQHLWHHQHEHP
                     PSPRGAATARQARCTFCTSCTSGTTGSGEITRMI"
     gene            5862..>6700
                     /locus_tag="PKK95_10985"
     CDS             5862..>6700
                     /locus_tag="PKK95_10985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC18787.1"
                     /translation="MSTPSPALRSAWQRHERRWADHLYVYAVVSRRSRGVSVGINLNP
                     GKECNFDCIYCQVDRGVPGRTRRVDLKVLESELDAILAAARDGSLYQSPPFDALAPDQ
                     RAVRDIAFSGDGEPTTFRGFGKAVEIAASARRRHGLDGAKLVLITNAAHLAKPQVREA
                     LALLDANNGEIWAKLDGGTAGSFQRVNRSNVTLEQVLDNILDAARVRPVVIQSLWMRL
                     DGEPPSDAEVVAFCARLEGLRASGGQVTSIQLYTVARKPAEPSVLPLNDAELDRVAGL
                     VRDR"
BASE COUNT         1027 a         2402 c         2318 g          953 t
ORIGIN      
        1 tggttctgag gtgccggagg tgctggtgct tgaggtgccg ggggtgctgg tgctgaggtg
       61 ccggggtgct gggggcctgc cgcaccgtgg ctacccgcac cgcgcgtcga aggcggtgca
      121 gtgctgaagg tgctgcgaac cagcgcggga gcttgcgcga tgcgcgcggc tgacgccggt
      181 gctggtgctg acagcaccgt cagcaccccc ggcacggcac ctccggcacc agcacctccg
      241 gcacttccag caccagcacc gtctgcacct ctgcaccacc tgcacctcag cacgacacgc
      301 atcagtggtg cttattccgc gcgcagcgcc gcgatggggt cgatcttcgt ggcacggcgg
      361 gccggcagat acccggcccc gagcgcgacg agggcgagca ccagcgtgcc ggcggcaaag
      421 gtcagcgggt cggaggccga gacgccgtag aggaagccgg agagcacccg cgtcgcggcc
      481 gcggccgcgg ccacgccgcc aatgagcccg agacccgcat agacgagacc gttttgcagc
      541 acgagccgga agaccgagcg cggcgacgcg ccgagcgcga cacgcacgcc aatctcctgc
      601 gttctctggg cgacagccgt ggcgagcacg ccgaagaggc cgatcgccgc gagcagaagc
      661 gccgaccccg cgaaggcggc aatcagcacg gtatagaacc gctgcgagcg caccgtcccc
      721 gtcaccaccc gctccatcgt cgtcacgttg gcgatcggaa gcgacgcatc catcgcgcgc
      781 acctcgcggc gcacggccgg cacgagcgac agcgggtcga tcccgcgcac cttgagcgcg
      841 acgtccatga agcggaagaa cgcaatctcc gcgtccgacg cctgggcgaa ggggcggtag
      901 actgacggcg tggccgcgct gtcgagaccg gcctgcttca cgtcggccac gacgccgacg
      961 atcgtcatcc acgccgcatc cgactgcgcg cctccccact tgatccgctc tccgaccgcg
     1021 cgcccgccgt cgaagaacag ccgggcgagc gtctcgttca cgatcaccac cggcggggcg
     1081 tcgggcttca tctcggcctc ggtgaaggcg cggcccgtga cgaagcggat cccgagggcc
     1141 gggaagtagt tgccgttgac gtacaccagg gtgttggagc ccgacacgcg attggccccc
     1201 ttggccgggc gctcgaccga gaacatccgg gtctcggtcg tgttcatcgg caggctcgtc
     1261 ccgccgccgg ccgcctcgac gccgggcagc cgcgccagcc ggctcaccag ctcgtcgtag
     1321 aacgtccgca cctgccgagc ctccgggtac gagctggccg gcagcgcggt cgagagcgag
     1381 agaacctgcg tgggcgagaa gcccggatcg gtggcgagca ggcgctcgaa gcttcgggcc
     1441 agcaggcctc cgccaatggc gaggaccagc gccagtgcga actgaactgc gaccaacccg
     1501 cccagcagcc gcgaggtgcg gggcccggtg gttgcgccgc gccccccctc gcgcagcgcc
     1561 tcgccccggt tgctgcgggc gaacgggatg gccggggcga gcccgaagat cagcgtcgtt
     1621 gcgagcgagg cggcaagcgc gaacgccagg acgctcgtgt ccacctgcac ggcctcgacc
     1681 cgcgggatgt tgacgggcag cgccgccacc aggacgtctg tgccccacat tgcgagcagc
     1741 acgcccacca cgccgcccgc aaggccgagc agcgccccct cggtcaggag ctgcgcgacg
     1801 aggcggcgcc gtccagcgcc catggcggca cgcagcgcga actcgcgctg gcgcgtggcg
     1861 ccgcgggcca gcagcaggtt ggcgacgttg gcgcagccga ccaggagcag cagcacgacc
     1921 ccgccgaaca gcacgaggat cagcgtgcgc acgccgccga cgatcgcctg cttgagcggc
     1981 gagaccgaca gctggagctt gaacttcggc tcggtgcgga ccccggcagg ataggcctcc
     2041 tcgagccgcg gaacgagccc ctcggcctcg gcccgggcct gctcgatggt gacgccaggc
     2101 cgcagccggc cgatcaccga cgagttgaac tggttgccgt agccctgcag gtcgtcgggc
     2161 gtgaaccact tgggaaggaa caggtcggcc ggctggttgt tgaaggcagc ccaagtcgct
     2221 ccctcgcccc ggggcgggaa ctccagccgc ggcccgagca cgccgacgac ctcgtgcggc
     2281 gtgcggtcga tgacgatcgt tcgcccgagg aggcggcgat cgccggcgaa ggtgcgccgc
     2341 cagagcgcgt cgctgagaat cacgacatgg cgcccctcgc cggcctcgcc gctcgcgaac
     2401 acccggccga gcgccggctc gatgccgagc agcgggaaca ggttcggcgt gacacgggca
     2461 gccacgaggc gctcgggctg cgcgacaccc gacagctcca cctgcctgtt gtcgtacgcc
     2521 gcgacgccct ccatggtccg gttccagcgc gagtaggcat cgaagtcggg cggcgagaag
     2581 gggacgacgg ggtagtcgag gcgcgggagt ccctcgctga tcgccaccag gcggtcgggc
     2641 tcgcgaaacg gcaacgggcg cagcagcacc ccgttggcca ggctgaagat gctggtcacg
     2701 gcgccgatgc cgaggccgag cgtgagcacc gcaaccaggg tcaccccggg cgtcttgccc
     2761 gccatccgaa gcgcgtgctt gaggtccgtc acgagaaagg acatgactgc atctcccggc
     2821 tggacgtgcc gacagtacct caggaaggcg aagaccgtac cgaaggccga acctcggcaa
     2881 atacttgagg aaacgggatt agagcgcagc gaccggcctc ccggccgttc ggaggcgcgc
     2941 acggacgtcc cggaatcgga cagctggaac gcgcgagatg cccaacgact ggcttgtcga
     3001 ggagcggtgc cctcagtgcg gcggcccggt gtcgatgcac gaggccgatc gcatcctcac
     3061 gtgcgcctac tgcaaggtgc gcctgtacgt gctcgcgggg ggcgccctcg cgaagtactg
     3121 cctgccggtg aagccggcgc caatcggcac catcgtgatg gtgccgtact ggcgcctgcg
     3181 ggcccaccgc taccggctgc tgccgtccgg cgtcgtgccc ggcgcagccg actgcacggc
     3241 cctcgccgcc gatgtgccgg gcctcgcctc gtcgctcggt gtgcgcgcac aggccgttcc
     3301 gcttcgtttc gcgacgccgg ctgccgaagg acggtttctg ccggcgacgc gcccgcccga
     3361 cctggtgttt cagcagctcg agcggctcag gcgcgtcgag caaggtcttg cgagtggccc
     3421 gcgcgcgagc gaggagcgcg aggccgcgcc gttcgaggcc accatcacca cggcctcctc
     3481 gctggtctac acgcccgtgc gagtgtcggc gcgctcggtg ttcgacgcgg tgctgaaccg
     3541 gagggttgcg gggatcgacg ccgacaggtg gaacgacgcg gtgccgggag ccgacgatcg
     3601 cccggcgccg gtgcggttcc tcgccacgct gtgcccggag tgcggctggc aactcgaagg
     3661 ggccaccggc acgctgatcc tgctctgcgt caactgcggc cgcgggtggc gagccggcaa
     3721 gtcgcagctc gtgccggctg acttcgacgt cctgccggcg gacggctgga cacccgacgc
     3781 gcacctgccg ttctggcgcg ccgaggcccg cgcggcggcc acctgggccg acttcgtgcg
     3841 cctggtcaac ctgccccgcg tcgtgcaggc ggcctgggag cgcgagccgc tgggcttctg
     3901 gttccccgcc ttcgccgcac accccgacca gttcctgcgc gccgcgcaga cactcacctg
     3961 cgcgaggccg gggcgacgcg aggcggcagc gacggaacag gcatcaacgc ccgcagagca
     4021 ggcgccggcg tctttgcagc cgaggaagtg ccgggcggtg acgctgccgg aagctgcact
     4081 ggacgaggcg ctgttcgtgc tgctggcaag cctcgggcac gcccgccgcg gcatgcggga
     4141 gatcctcgcc gggatcgatc tccacgtcca cgagcgccgc ctggtttacg tgcccgcccg
     4201 cttcaacggc cgcgaactgg tgaaccccga actggggctc acggtgcaga gaagtcttcg
     4261 gtcttcgagc ttcagctccc agctttgagg ctcacgtcgg ctccgggaat cgcccgctcg
     4321 atctcggcgg cgtcggtgtc gggaagctgg aagtcggcgg ccttgacgat ctcggccacc
     4381 tgggcaggcc ggcggaagcc ggcaatcgcg gccgtgacgg ccgggtggcg gagggtccag
     4441 gcgatcgcgg cttccccggg cgtccggcca tgccgggccg caaccgtgcg gagtacctcg
     4501 acgaaggcca ggttggcgct gaggttcggc tcctggtact ccctcgtccg cttcctccag
     4561 tcgtcctccg gcagcgcggc gatgcgctcg cgggtcatct tgccgctcag gaggccggcc
     4621 tgcatcgggg aatagacgat gacgccgatg tcgtgcgcga gacagtacgg cagcacctcg
     4681 gcctcgatct tccggcgcag cagcgagtag ggcggctgca gcgactcgac cggtgccacg
     4741 cggttggcgc gctcgaggtc gtccacgccg aagttcgaca cgccgatgtg cctgaccttg
     4801 ccctcccgct tcagctcggc cagggctccc cacccctcct cgaggcgatc cgcggcggcg
     4861 tcgggagcat tcgtccaccg cggccagtga acctggtaaa ggtcgatggc ctcgacgccc
     4921 agccggcgca ggctggcctc gcactcgcgc ctgatggacg aggcgtcgag cgagtgcgtg
     4981 acctcgcgcc caccctcggg ccagacgagg ctgcacttgg tgaagacgta gggccgcatt
     5041 ccggcgggga tgcgctcgag ggcgcgaccg accacctctt ccgaccggcc gaggccgtac
     5101 accggagcgg tatcgatcca gttgacaccc aggtcggccg ccttctcgat tgcggcaatc
     5161 gactcgttgt cgtcctgcgg cccccagccg aacgcgtagc cgccgccgcc aacggcccag
     5221 gccccgaacc cgacgcgcgt gatctcgagt cccgtcctgc caagtcgctt cttctgcatc
     5281 ggtcgcctcc tgatcccgga gatctccggc gcccgtgagt ttctgcggtg aggtacgtca
     5341 cagacggaac ggctcgtcca cgttcaccac agaggcacag aggcacggag atgcacgtag
     5401 aggtttcaga tcattctagt aatctcgccc gatcctgtcg tgccggaggt gcaggaggtg
     5461 cagaaggtgc agcgggcctg ccgcgccgta gccgcgccgc gcggcgaagg cgggtgctcg
     5521 tgctggtggt gccacaggtg ctggtgcagc gggtgtgggc gcggcactct cggcaccagc
     5581 tcttccggca ccatcagcac cttcagcacc tcctgcactt gcaccttcag caccccctgc
     5641 actgcacctt cagcacctcc tgcacttgca ccttcagcac ctcctgcact gcaccttcag
     5701 caccccctgc actgcacctt cagcacccat gcactgcacc ttcagcacct cctgcactgc
     5761 accttcagca cctcctgcac ttgcaccttc agcacctcct gcaccttctg cacctcctgc
     5821 acgatctgat ctccaacccc ttcgttgtta tcatcgtgaa gatgtcgacc ccttcgccag
     5881 ccctgcggtc ggcgtggcag cggcacgagc gccgctgggc cgatcacctc tacgtgtacg
     5941 cggtcgtctc caggcgaagc cgcggggtct cggtggggat caacctcaat cccgggaagg
     6001 aatgcaactt cgactgcatc tactgccagg tcgatcgcgg agtgccggga aggacgcggc
     6061 gggtggacct caaggtgctc gagagcgagc tcgacgccat cctggccgcg gctcgtgacg
     6121 gctcgctgta ccagtccccg ccgttcgacg cgctggcgcc cgaccaacgt gccgtccgcg
     6181 acattgcctt ctccggcgac ggagagccga ccaccttccg cggcttcggc aaggcggtgg
     6241 agattgcagc gtccgcgagg cgccggcacg gcctcgacgg ggcaaagctg gtgctgatca
     6301 ccaacgcggc gcacctcgcg aaaccgcagg tccgcgaggc gctggcgctc ctcgacgcga
     6361 acaacggcga gatctgggcc aagctggacg gcggaaccgc ggggtcgttc cagcgggtga
     6421 accggtcgaa cgttacgctc gagcaggtgc tggacaacat cctcgatgcg gcgcgcgtgc
     6481 gccccgtggt gatccagtcg ctctggatgc gcctcgacgg cgagccgccc tccgacgccg
     6541 aggtcgtcgc gttctgcgcc aggctcgaag ggcttcgcgc gtcgggcggg caagtcacgt
     6601 cgatccagct ctacacggtc gcgcgcaagc cggccgagcc gtcggtcctg ccgctgaacg
     6661 acgccgagct cgaccgggtt gcgggcctgg tgcgcgaccg
//
LOCUS       DAOPQX010000198         6681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12619_length_6681_cov_5.183369, whole genome shotgun sequence.
ACCESSION   DAOPQX010000198 DAOPQX010000000
VERSION     DAOPQX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12619_length_6681_cov_5.183369"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="PKK95_10990"
     CDS             complement(<1..1715)
                     /locus_tag="PKK95_10990"
                     /inference="COORDINATES: protein motif:HMM:NF023375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3466 family protein"
                     /protein_id="HOC18788.1"
                     /translation="MWRSTRVRAVLAGIVLACSLFAAGSPVATQSPTGTWTAIDLGTL
                     GGATASANGVGDLGEFAAGSSVTASGARHAFVWRALNGMRDLGTLGGRESEAVFVTTG
                     WRIVGRAQVASGRFHAFAAEFNRPLTDLGTLGGSESAAYGANDWGAVVGSSKTAGDAH
                     TTAFVYQDGAMTALDLPWSDDHAALAINDEGQIVGYVDTADRGRQAFLYEDGAATLLA
                     QALGSSSVATAINRAGQIVGFFSNGDTSGGAFIHADGVSAALPGLGGAFAFARAINDE
                     GDIVGEADTSSGARHAVLWRDRVATDLNSLLAPGSPWVLHAATGIDQYGNIVGNATLD
                     GEPRAFLLVPPIDIAISLSRHENEIDTNIPRPHEAGRLLTLGVSVYHDQPHPLTGVVI
                     SDELSGPVEYVDWDTTEWDCTRSGQRLTCRLRQTISPGGRGRDLMLTVRATAAGEITH
                     RATVRADAFDPDLSDNSAVETNVAVSLASLTLNSPIVGGKPSLARATITTGNVPGGAR
                     VTLASSNPAIATVPSAFDVLEGTWREFYVTTQPVSQVTTVLISATYGLVTITQPLTII
                     PVGDPS"
     gene            1988..2995
                     /locus_tag="PKK95_10995"
     CDS             1988..2995
                     /locus_tag="PKK95_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC18789.1"
                     /translation="MVFAADPYVAPPALRNGHRMTLYAWARRRRFPRLPEPEARLFDV
                     APQTRVLAKCHWQDRRAEHPTFLLLHGLEGSADAHYMRGLADKGWAAGFNVVRLNQRN
                     CGGTDHLAEGVYHSGLTADPMAVIRELIDHDRLRSIVVAGYSLGGNLSVRMAGEYGDA
                     VPAEVKGFAAVSPTIDLMSCIEALERRSNWIYQWNFMRDLKGRIRRKVRLYPESYSVA
                     GLRRLRTVRQFDDAYTAPMSGYRDAADYYHRASALRVIANARVPTLILSAEDDPFVPA
                     EQFRHPDVAGNPHVKVVLTRHGGHCGFIEASRDGYDGYWAERTVVKWGQQADKTRGGG
                     S"
     gene            2992..3588
                     /locus_tag="PKK95_11000"
     CDS             2992..3588
                     /locus_tag="PKK95_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18790.1"
                     /translation="MTEATLPAVGQGPAWVTAEMPPGYQNRLFEIQRLTADLREMDQI
                     ARVLWETGQPLEQAVAAILGAVECEVDAERGTAGALVATIGSSRRLLLVVSGGAGPIE
                     KTSEELTRAFQAVQFASPGDRVVLVGGNDPNTPPADRPAPVLPEALDIVERMGVNVTT
                     TATVFRLWRLSLEDKPKARKALDQLHAQDGGVFVIPAR"
     gene            complement(3607..6114)
                     /locus_tag="PKK95_11005"
     CDS             complement(3607..6114)
                     /locus_tag="PKK95_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18791.1"
                     /translation="MATLIHDLRLALRGLRRSPLFTAVAVLSLALGIGANAAIFRLID
                     QVLLRKLAVREPGELVMLHQEGPHMGSNMGSRAHSYPLYRDFQQKAEPLSEVICRRLV
                     PVSVTVGQQTERVESELVSGNYFTMLGVRPAVGRVFSSREDDQVYLGHPVVVLSHGYW
                     VSHFNRDRGVVGRKIRVNDYPMTIVGVSEEGFTGLDPSRSPHLRVPILMQPVIMPTVT
                     WLQMENRRSRWVQVFGRLKPGYTLESARAPLQGLFTQIRAYEMTLAAAKDWSAYHRAE
                     FMKGRLVVTPAAVGYSPLRNDFSKALVVLMCMVGLVLLIACANVANLLIARGFSRQKE
                     IAVRLSLGSSRARLVSQLLAESLVLSTAGGVAGLALAVGLTRGLLALLPSQGQPLLIS
                     PYPDWRLLGFTLALATATGLVFGLLPALRASRWDPWTTLKDTAGAVAGTRGSGFLRKS
                     LVVAQVALSFLLLAGAGLFVRSLQNLKATETGVAVDNLVAFELSPALSGYDDERATRF
                     YRELLDRLRSAPGVHSAAFAAVPILAGDEWDSSMAVEGHTAKDGEDMQMWMNMISPGY
                     FKTMKVPILEGRDFRPDDAVPFEQIKTAIVNKRFADHFFPGRSAVGKRIGWEDGPDTK
                     LTIEIVGVAGNALYEGPREGLRRQVYIPNWGKESAVFYLRTLTPAGSVHSLVRNEVRR
                     LDAGIPVFGMKTLEAQLDETLLTDRLIAMLSAGFGLLATLLASIGLYGVMAFMVARRT
                     KELGIRMALGAQARSVTWIVMREVLLLLAAGLAIGVPVSLGLGRLVSAQLFGVRPHDP
                     AIAMLTVVLLAAVATAAGMIPARRASRIDPILALREE"
     gene            6187..>6681
                     /locus_tag="PKK95_11010"
     CDS             6187..>6681
                     /locus_tag="PKK95_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC18792.1"
                     /translation="MRHDFDRIVDRRNTLSLKWDHCEQCFGIEDVIPMWVADMDFEAP
                     PAVAEALRSRAAHPIYGYASTPDSFWQAAIDWLDRRHGWKVERKWLARSPGVVPALSL
                     CVNAFTEPGDGVVVQPPVYYPFFWAVENNGRRLVRNPLAAGAGGRYVMDLAGLESKLD
                     ERTRL"
BASE COUNT         1002 a         2305 c         2399 g          975 t
ORIGIN      
        1 gacggatccc cgaccgggat gatcgtgagc ggctgggtga tggtgaccag gccgtaggtg
       61 gcgctgatga gcaccgtcgt cacctggctg accggctggg ttgtcacgta gaactcgcgc
      121 cacgtcccct cgagcacgtc gaaggccgac ggcacggtgg caatcgcggg gttgctgctc
      181 gccagcgtca ccctggcgcc gcccggcacg ttgcccgtgg tgatcgtcgc cctcgcgagc
      241 gagggtttgc cgccgacgat gggcgagttc agggtgagcg acgcgaggct cacggcaacg
      301 ttcgtctcga ccgcggagtt gtcggagagg tcgggatcga aggcgtcggc gcgaacggtg
      361 gcgcggtgcg tgatctcacc ggccgcggtg gcgcgcacgg tcagcatgag gtcgcgtccc
      421 cgcccgccgg gggagatcgt ctgccggagc ctgcaggtga gccgctggcc cgaccgggtg
      481 cagtcccatt cggtcgtgtc ccaatcgacg tactcgaccg gccccgacag ctcgtcgctg
      541 atcaccacgc cagtcagggg gtgcggctga tcgtgataga cgctgacgcc gagcgtcagc
      601 agccgccccg cctcgtgggg cctggggatg ttggtgtcga tctcgttctc gtgccggctc
      661 aggctgatgg cgatgtcgat cggcggcacc agcaggaacg cccggggctc gccgtcgagc
      721 gtcgcgttgc cgacgatgtt gccgtactgg tcgatgccgg tggccgcgtg cagcacccag
      781 gggctgccgg gcgcgagcag cgagttgaga tcggtcgcca cccggtcgcg ccacagcacg
      841 gcgtgccgcg cgccgctgct cgtgtcggcc tcgccaacga tgtcgccctc gtcgttgatg
      901 gcgcgggcaa aggcgaaggc gccgcccagg ccggggaggg ccgccgacac cccatcggca
      961 tggatgaacg cgccgcccga ggtgtcgccg ttcgagaaga agccgacaat ctgccccgca
     1021 cggttgatgg cggtcgcaac gctcgacgag ccgagcgcct gcgccagcag ggtggccgcg
     1081 ccgtcctcgt agaggaaggc ctggcggccg cggtcggcgg tatccacgta gccgacgatc
     1141 tggccctcgt cgttgatggc gagggcggcg tggtcgtcac tccaggggag gtcgagcgcg
     1201 gtcatcgcgc cgtcctggta gacgaaggcc gtggtgtgcg cgtcgccggc ggtcttcgac
     1261 gacccgacga ccgcgcccca gtcgttggcg ccgtacgcgg cgctctcgga gccgccgagg
     1321 gttccgaggt cggtgagcgg gcggttgaac tcggccgcaa aggcgtggaa ccggcccgag
     1381 gccacctggg cgcggcccac gatccgccac ccggtggtga cgaagacggc ctcgctctcg
     1441 cggcccccga gggttcccag atcgcgcatg ccgttgaggg cgcgccacac gaaggcatgc
     1501 cgcgcccccg aggcggtgac gctgctgccg gcggcgaact caccgaggtc gccgacgccg
     1561 ttggcggacg ctgttgcgcc gccaagggtc ccgaggtcga tggcggtcca cgttccggtc
     1621 ggcgactggg tggcgacggg cgagccggcg gcgaacaggc tgcacgccag cacgatcccg
     1681 gcgagcaccg cgcggacgcg tgtcgagcgc cacatgggtc cctcctgcag gtggtgttgt
     1741 gggggcgagc gcgtacggtt gtgcaggtag cgtgcccggc gggccgcgca ggattgtgaa
     1801 ggatcgcgca agtgccggtg cggggcgcgg gccgtcgtgt cgtgttccca atcagtcgag
     1861 cttcacgcct gccgataacg gacgggctcg cgccagccgt cagggcgacc acccgccttc
     1921 gcctccacga ggcttcggcg cgccgccgga ctggcgcggg aaccggaatt gggattgaat
     1981 cgaggcgatg gttttcgccg ccgatcctta cgtggccccg cccgccttgc gcaacggcca
     2041 ccgcatgacc ctctacgcct gggcccggcg gcggcgcttc ccgcggctgc ccgagcccga
     2101 ggcccggctg ttcgacgtgg cgccgcagac gcgcgtgctg gccaagtgcc actggcagga
     2161 ccggcgggcc gagcacccca ccttcctgct gcttcacggc ctcgagggat cggccgacgc
     2221 ccactacatg cgcggcctgg ccgacaaggg ctgggccgcc ggcttcaacg tcgtcaggct
     2281 gaaccagcgc aactgcggcg gcaccgacca cctggccgag ggcgtgtatc actcgggcct
     2341 caccgccgac cccatggccg tgatccgcga gttgatcgac catgaccgcc tccggtcgat
     2401 cgtcgtcgcc ggctactcgc tcggcgggaa cctgtcggtg cgcatggccg gagagtacgg
     2461 cgacgcggtt ccagcagagg tgaaggggtt cgcggcggtg tcgccgacga tcgacctgat
     2521 gtcgtgcatc gaggcgctcg agcgccggtc gaactggatc taccagtgga atttcatgcg
     2581 cgacctgaag gggcgcatcc ggcggaaggt gcgtctctac ccggagagct actcggtggc
     2641 cggcctccga cgcctccgca ccgtccgcca gttcgacgat gcctacacgg cgccgatgag
     2701 cggctatcgc gacgcggccg actactacca ccgcgcgagc gcgctgcgag tgatcgccaa
     2761 cgcccgcgtc cccacgctga tcctgtcggc cgaggatgat ccgttcgtcc ccgcggagca
     2821 gttccgccac cccgatgtcg ccggcaaccc gcacgtgaag gtcgtcctca cgcgccacgg
     2881 cggccactgc ggcttcatcg aagcgtcgag ggacggctac gatggctact gggccgagcg
     2941 gacggtggtc aagtgggggc agcaggccga taagacccgg ggaggtggtt cgtgaccgaa
     3001 gcgacgctgc cggcggttgg acagggaccc gcctgggtga cggcggagat gccgcccggc
     3061 tatcagaatc gactcttcga gatccagcgc ctgacggccg atctccgcga gatggaccag
     3121 atcgcgcggg tgttgtggga gaccggccag ccgctcgagc aggctgtcgc cgcgatcctc
     3181 ggcgcggtcg agtgcgaggt ggacgccgag cgcggcaccg ccggggcgct ggttgccacg
     3241 atcggttcct cgcggcgttt gctgctcgtg gtctcgggcg gagcgggacc gatcgagaag
     3301 acgagcgaag agctgacgcg ggcgttccag gccgtgcagt tcgcctcgcc cggcgaccgt
     3361 gtcgtgctgg tgggtgggaa cgatcccaac acgcctcccg ccgatcggcc cgccccggtc
     3421 ctgcccgagg cgctcgacat tgtcgagcgg atgggcgtga acgtgacgac gaccgccacc
     3481 gtgttcaggc tgtggcgcct ctcgctcgag gacaagccca aggcgcggaa ggccctcgat
     3541 cagctgcacg cgcaggacgg gggagtcttc gtcatcccgg cgcgctaggg aatttccacc
     3601 ctcggctcat tcctcgcgca acgccaggat cggatcgatc cggctcgcgc ggcgcgcggg
     3661 gatcatcccg gccgccgtgg cgaccgccgc gagcagcacc acggtgagca tggcgattgc
     3721 cgggtcgtgc ggcctgacgc cgaagagctg ggccgagacc aaccggccga gcccgagcga
     3781 aacgggaacg ccgatggcga ggccggcagc gagcagcagc agcacctcgc gcatcacgat
     3841 ccaggtgacg ctccgggcct gcgcgccgag cgccatgcgg atccccagct ccttggtccg
     3901 ccgggcgacc atgaaggcca tcaccccgta caggccgatc gaggcgagga gtgtggcgag
     3961 caggccgaac cccgccgaca gcatcgcgat cagccggtcg gtgagcagcg tctcgtcgag
     4021 ctgggcctcg agggtcttca tgccgaacac cgggatcccg gcgtcgaggc gccgcacctc
     4081 gttgcggacc aggctgtgca cgctgccggc cggcgtcagc gtgcgcaggt agaagaccgc
     4141 gctctccttc ccccagttcg ggatgtacac ctggcggcgc agcccctcgc gcggcccctc
     4201 gtacaacgcg ttgccggcaa cgccgacgat ctcgatcgtc agcttcgtgt cggggccgtc
     4261 ttcccagccg atgcgcttgc cgacggcgct gcgtcccggg aagaagtggt cggcgaagcg
     4321 cttgttgacg atcgccgtct tgatctgctc gaagggcacc gcatcgtctg gcctgaagtc
     4381 gcgcccctcg aggatcggca ccttcatcgt cttgaagtag ccgggcgaga tcatgttcat
     4441 ccacatctgc atgtcctcgc cgtccttggc cgtgtgtccc tcgaccgcca tcgagctgtc
     4501 ccactcgtcg ccggcgagga tcggcaccgc cgcgaaggcc gccgagtgca ccccgggcgc
     4561 cgatcgcagc cggtcgagga gctcgcggta gaaccgcgtg gcccgctcgt cgtcgtagcc
     4621 gctcagcgcc ggcgacagct cgaacgcgac caggttgtcg acggccacgc ccgtctcggt
     4681 ggccttcagg ttctgcaggc tgcgaacgaa caggcccgcg ccggccagca gcaggaagct
     4741 gagggcgacc tgcgccacga cgaggctctt ccggaggaac cccgatcccc gcgtgcctgc
     4801 gacagcgccc gccgtgtcct tcagcgtggt ccaggggtcc cagcggctgg cccggagcgc
     4861 cggcaggagg ccgaacacca ggcccgtggc cgtggccagg gccagcgtga acccgagcag
     4921 gcgccagtcc gggtacgggg aaatcaacag cggctgtcct tgcgacggca acagggcgag
     4981 gaggccgcgc gtcaggccga ctgcgagggc gaggcccgcg acgccgcccg cggtcgacag
     5041 caccaggctc tccgcgagca gctggctgac gaggcgcgcg cgcgacgaac cgagcgacag
     5101 ccgcacggca atctccttct ggcgcgagaa gccgcgggcg atcagcaggt tcgccacgtt
     5161 ggcgcaggca atcagcagca ccaggccgac catgcacatc aggacgacca gcgccttgga
     5221 gaagtcgttc cgcagcggcg agtagccgac ggccgccggc gtcacgacca gccgcccctt
     5281 catgaactcg gcgcggtggt acgccgacca gtccttcgca gcggcgagcg tcatctcgta
     5341 ggcgcggatc tgcgtgaaga gcccctggag cggcgcccgc gccgactcca gcgtgtagcc
     5401 gggcttcagc cggccgaaca cctgaaccca cctcgagcgg cggttttcca tctgcagcca
     5461 ggtcaccgtc ggcatgatca ccggctgcat caggatcggc acccgcaggt gcggcgagcg
     5521 cgatgggtcg aggccggtga acccttcctc ggacacgccg acgatcgtca tcgggtagtc
     5581 gttcacccgg atcttgcgcc ccacgacgcc ccggtcgcgg ttgaagtggc tcacccagta
     5641 cccgtggctc agcacgacca cggggtgtcc caggtagacc tggtcgtcct cccgtgagct
     5701 gaacacccgc ccgaccgcgg gcctgacgcc cagcatggtg aagtagttgc ccgagaccag
     5761 ctccgactcc acccgctcgg tctgctggcc cacggtgacc gagaccggca ccagccggcg
     5821 gcagatcacc tcggagagcg gctcggcctt ctgctggaaa tcacggtaga gcgggtacga
     5881 gtgtgcccgc gaacccatgt tgctgcccat gtggggccct tcctggtgca gcatcaccag
     5941 ctcgccgggc tcccggacgg ccagcttcct gagcaggacc tggtcgatca gccggaagat
     6001 cgcggcgttg gcgccgattc ccagggccag ggagaggaca gccacggcgg tgaagagcgg
     6061 gctgcggcgc aggccgcgga gcgccaggcg caggtcgtgg atgagggtgg ccatggacga
     6121 ttggacgggc cagggacctg gagggttcgc acggtcgaga ggctgccgtg atgtaacctc
     6181 tttcgcatgc gccacgactt cgaccgcatc gtcgatcgcc ggaacaccct gtcgctcaag
     6241 tgggatcact gcgagcaatg cttcgggatc gaggacgtca tcccgatgtg ggtggccgac
     6301 atggacttcg aggcgccgcc cgcggtggcc gaggcgctgc ggtcgcgcgc cgcccacccc
     6361 atctacggct acgcgtccac gcccgattcc ttctggcagg ccgcgatcga ctggctcgac
     6421 aggcggcacg ggtggaaggt cgagcggaag tggctcgccc ggtcgcccgg cgtggtgcct
     6481 gcactcagcc tgtgcgtcaa cgcgttcacc gagccgggcg acggcgtggt cgtccagccg
     6541 ccggtgtatt acccgttctt ctgggcggtc gagaacaacg ggcggcggct ggtgcgaaac
     6601 ccgctggcgg ccggggccgg cgggcggtac gtcatggacc tcgccggcct cgagtcgaag
     6661 ctcgacgagc ggacgcgcct c
//
LOCUS       DAOPQX010000199         6653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12676_length_6653_cov_4.985905, whole genome shotgun sequence.
ACCESSION   DAOPQX010000199 DAOPQX010000000
VERSION     DAOPQX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12676_length_6653_cov_4.985905"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PKK95_11015"
     CDS             complement(<1..829)
                     /locus_tag="PKK95_11015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC18793.1"
                     /translation="MQPGTIVSHYRIAEQLGEGGMGVVFKAEDLALGRPVALKFLPAD
                     LAEEEPNVARFEGEARYLSAASHPNICTIYEIGSHEGRPFIAMELLEGEPLHHVIDRG
                     PAPLNLILEIGIAVADALEAAHQARLVHRDVKPGNIFITTRGQVKVLDFGLAKPMQAA
                     PAGEDALPALTQTGLTMGTVSYMSPEQARGEPLDGRSDIFSLGLVLHEMATGQQTYGG
                     ATAAVVFDALLNRPPAAIGPINPSLPPALEAIIGRALEKDPGARYQRAAELRDALDEL
                     "
     gene            complement(945..1874)
                     /locus_tag="PKK95_11020"
     CDS             complement(945..1874)
                     /locus_tag="PKK95_11020"
                     /inference="COORDINATES: protein motif:HMM:NF018731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 domain-containing protein"
                     /protein_id="HOC18794.1"
                     /translation="MPTDRLAALSAVLDGLMSRYRAQVPDVQEIVREMVREGIIPAEE
                     ALENDHIAFRTLGVPNLGIASLERVFLHYGYQRRDRYHFAEKKLDAHWYAPPAERFPR
                     IFISQLRVGELSPEAQRIVHSYTDLVREDPVGRLDLDDAPAVVAFLHAPLWRTPTWAD
                     YVRLRDESEYAAWVIYNRYYLNHFTIAVHNLPGAYGSLEEFNRFLELHGFTLNDSGGK
                     VKTSPDGKLLQSSTVAAMVDAEFDDGRGGVERKRIAGSYVEFAERRVLDEFTHLPPHE
                     VRREHRREGFESGNADRIFESTYTAQTGKRDGA"
     gene            complement(1920..2327)
                     /locus_tag="PKK95_11025"
     CDS             complement(1920..2327)
                     /locus_tag="PKK95_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18795.1"
                     /translation="MALRELLLPVPPRRLAHARAWNVVFRTAHIAVVGTLLGGHVFGL
                     PHDRLIGWLWASIATGAVLMAIEMYATLDWLMQVSGLLVVLKLLLLLLILLAWDARVP
                     ILYTVVAIAGIGSHMPGKYRHYSLYYRRNTRGK"
     gene            2418..2879
                     /locus_tag="PKK95_11030"
     CDS             2418..2879
                     /locus_tag="PKK95_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18796.1"
                     /translation="MYETVLALHSVLRWLVVLAGIWAVAAAFAAARRREWTPADNRPG
                     LLFVLGLDVQLLLGLALFLFLSPITRAAMLDMGTAMRSSASRFWIVEHPTLMIAAVVL
                     GHVGRVAARRAGSYRAGNRAQFWFALALVAVLLATPWPFVGHARPWFRMPF"
     gene            complement(2896..3381)
                     /locus_tag="PKK95_11035"
     CDS             complement(2896..3381)
                     /locus_tag="PKK95_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOC18797.1"
                     /translation="MLISKELNAAINEEIGREFFASHQYTNIACYFESLALRKLAGMF
                     HKQADEERMHALKFVKYLNDVGATVEIPAIPAPQATFKSVEEAVQKSLDWEREVTRNC
                     NALMAMAVDQKDFAAQDFLRWFVTEQVEEEATMGTLLQVVKAAGERNLIMVEAYLVHQ
                     D"
     gene            3522..4961
                     /locus_tag="PKK95_11040"
     CDS             3522..4961
                     /locus_tag="PKK95_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOC18798.1"
                     /translation="METVALPSPNAADQAAFLAAHPEYEATRGIDELRAREFARLDAH
                     GDVYLDYTGGGLYAASQVRAHAEQLLAHVFGNPHSSNPTSLRSSEHVERCRADVLRYF
                     NAPRDEYTVIFTNNASQALKLVGESYPFEPGSTLLLTFDNHNSVNGIREFARSRGART
                     VYLPVFPPDMRADPALVAGRLDALASPAPNLFAYPAQSNFSGVQHPLEWVARAQERGW
                     DVLLDAAAFVPANRLDLSAVQPEYLVLSFYKMFGYPTGVGALLARRRALEKLHRPWFA
                     GGTITVASVQADRHYLAPGSAAFEEGSPSYLTIPAVEFGLSLVESVGIDTIHTRVRCL
                     AGWLIDALLSLLHDNGEPLVRLYGPANTTARGATLALNFYDRIGRALDHRLIERQANR
                     ERISLRTGCFCNPGAGETALGLTKGELDSCFLHSSRRMTFDQFRSCIDGRSSGAVRVS
                     LGLASNFDDVLAMRNFARQFLDWENGPED"
     gene            complement(4993..5802)
                     /locus_tag="PKK95_11045"
     CDS             complement(4993..5802)
                     /locus_tag="PKK95_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOC18799.1"
                     /translation="MLIQVDEAADPRLADYRGVAEPARLRRDGLLVAEGRQVVRVLLA
                     VPRFRVRSLLVNEAALDALADQVVPRLKDLDVYLAPREIFAALTGYDINRGCLAIAER
                     PASADVGEWLGERPDARLLVGAEGVANPDNLGGLFRNALAFGADALLVGPSCADPLYR
                     KTIRVSMGAALRVPFVEATGWPQCLEMLAGRGFAVIALTTDPSAVPIEDVARRPPERF
                     VLVAGSEGAGLTPAALAAATLQVRIPMAPGVDSLNVATATGIALHRLRPEP"
     gene            complement(5807..>6653)
                     /locus_tag="PKK95_11050"
     CDS             complement(5807..>6653)
                     /locus_tag="PKK95_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18800.1"
                     /translation="PPLPGAPPSGETGVPVQKKTGDIELVAESVLQHTLLVTWYGNPH
                     SKRMGILGEHTGDELASGLRRQADAYAALTDKRVQPAYELVAVVAQNNPGRDGTWRRR
                     ESRAVIERMLAEARAHGFALVLDVQVGHSTVEAELEYLRRYLEEPDVHLALDPEFDMP
                     NGEPPGTRIGTTPARDVNYAIDLLERIVIEKKLPPKVLIAHQFTMNMLPDKRNVQDSP
                     VVDVALVMDGWGPRQLKHAIYRMVTRTPLEHSGIKLFYRKEPDLLSPSHVLELKPTPS
                     IVIYQ"
BASE COUNT          950 a         2335 c         2317 g         1051 t
ORIGIN      
        1 gcagctcgtc gagcgcgtcg cgcagctccg cggcccgctg gtaacgcgca ccgggatcct
       61 tctcgagcgc gcgcccgatg atcgcctcga gggccggggg gagcgacggg ttgatggggc
      121 cgatcgcggc aggcggccgg ttcagcagcg cgtcgaacac cacggcggcc gtggcgccgc
      181 cgtaggtctg ctggccggtc gccatctcgt ggaggacgag gccgagcgag aagatgtcgc
      241 tgcggccgtc gagcggctcg ccccgggcct gctccggcga catgtacgac acggtcccca
      301 tcgtgaggcc ggtctgcgtg agggcgggca gcgcgtcctc ccctgccggc gcggcctgca
      361 tcggcttggc gaggccgaag tcgagcacct tcacctggcc ccgggtggtg atgaagatgt
      421 tccccggctt cacgtcccgg tggaccagcc tcgcctggtg cgcggcctcg agggcgtcgg
      481 ccacggcaat cccgatctcg aggatcaggt tcagcggcgc cggcccccga tcgatcacgt
      541 ggtggagcgg ctcgccctcg agcagctcca tcgcgatgaa ggggcgcccc tcgtgcgaac
      601 cgatctcgta gatcgtgcag atgttcgggt ggctcgccgc cgagaggtag cgcgcttccc
      661 cctcgaagcg cgcgacgttc ggctcctcct ccgccaggtc ggcgggaagg aacttgaggg
      721 caaccggccg gcccagcgcg aggtcctcgg ccttgaagac gacgcccatg ccgccttcgc
      781 cgagttgctc ggcaatcctg tagtgcgaaa cgatggtgcc cggctgcatg ctctcgcacg
      841 cagtctatgg acgcgcgccg gccgaagtca accatccggc cggcactcct gccgggcgga
      901 cggcggcccg cggccagccc gggctggcgg ggagcctgcc gccgctacgc gccgtctcgc
      961 ttcccggtct gcgcggtgta cgtgctctcg aaaatccggt cggcgttgcc cgactcgaac
     1021 ccctcgcgcc ggtgctcgcg gcgcacctcg tgcggcggca ggtgggtgaa ctcgtcgaga
     1081 acgcggcgct ctgcgaactc gacgtacgac ccggcaatcc gcttccgctc gacgcccccc
     1141 cggccgtcgt cgaactcggc gtccaccatc gccgccaccg tggagctttg cagcagcttg
     1201 ccgtccgggc tcgtcttcac cttgccgccc gagtcgttga gggtgaaccc gtgaagctcg
     1261 aggaagcggt tgaactcctc gagcgagccg taggcgccgg gcaggttgtg caccgcgatc
     1321 gtgaagtggt tcaggtagta ccggttgtag atcacccagg cggcgtactc gctctcgtcc
     1381 cgcagccgga cgtagtcggc ccacgtgggc gtccgccaga gcggcgcgtg caggaatgcc
     1441 actaccgccg gggcatcgtc caggtcgagc cgcccgaccg ggtcttcgcg gacgaggtcg
     1501 gtgtaggagt ggacgatccg ctgcgcctcg ggtgacagct cccccacgcg cagctgggag
     1561 atgaagatgc gcgggaagcg ctcggcgggc ggggcgtacc agtgcgcgtc gagcttcttc
     1621 tcggcgaagt ggtaccggtc gcgccgctgg tagccgtagt ggaggaacac ccgctcgagc
     1681 gaggcgatcc ccaggttggg cacgcccagg gtgcggaagg cgatgtggtc gttctccagc
     1741 gcctcctcgg cggggatgat cccctcgcga accatctcgc ggacgatttc ctggacgtcg
     1801 ggcacctgcg cccggtagcg ggacatgagg ccgtcgagca ccgctgacag cgctgcgagc
     1861 cgatcggtgg gcatgggtgg ttgcgctccc aatcctcagc ctaccacatc ggccggcggt
     1921 cacttcccgc gggtgttcct gcggtagtag agcgagtagt gccggtactt gcccggcatg
     1981 tgcgagccga tcccggcgat cgcgaccacc gtgtaaagga tcggaacgcg ggcgtcccag
     2041 gcgaggagaa tcaggagcag cagcagcagc ttcagcacga cgagcagccc gctgacctgc
     2101 atgagccagt cgagcgtcgc gtacatctcg atcgccatca gcacggcgcc ggtcgcgatc
     2161 gacgcccaca gccacccgat cagccggtcg tgcggcaggc cgaagacatg accgccgagg
     2221 agggtgccga cgacggcgat gtgcgcggtg cggaagacga cgttccaggc gcgggcgtgg
     2281 gcgaggcgcc gcggcggcac cggcagcagg agttcacgca aggccatgaa ggaggtagtg
     2341 taacgaagaa cggccgcctc gcggcggccg accccgttca tttgcccgcc gaggggctcc
     2401 cgaactagga tgagcccatg tacgagacag tactcgccct gcactcggtg ctgcggtggc
     2461 tcgtggtcct cgccggcatc tgggccgtgg ctgccgcctt cgccgcggcg cgccggaggg
     2521 agtggacgcc ggccgacaac cggccggggt tgctgttcgt gctggggctc gacgtgcagc
     2581 tgctgttggg gctggcgctc ttcctcttcc tgagcccgat cacgcgcgct gcgatgctcg
     2641 acatgggcac ggcgatgcgc agcagcgcgt cgcgcttctg gatcgtcgag cacccgacgc
     2701 tgatgattgc cgccgtggtg ctggggcacg tgggccgggt cgcggcgcgc cgggcgggca
     2761 gctaccgcgc cgggaaccgc gcccagttct ggttcgcgct cgccctggtc gcggtgctgc
     2821 tcgccacgcc gtggccgttc gttggccacg cccgcccgtg gttccggatg ccgttctgac
     2881 gcgtcgagcg gcctgctagt cctggtgcac caggtacgcc tcgaccatga tcaggttccg
     2941 ctcgccggcc gccttcacca cctgcagcag ggtccccatc gtcgcctctt cctccacctg
     3001 ctcggtgacg aaccagcgga ggaagtcctg ggcggcgaag tccttctgat ccaccgccat
     3061 cgccatcagg gcgttgcagt tgcgcgtgac ctcgcgctcc cagtcgagcg acttctgcac
     3121 cgcctcttcg accgacttga aggtggcctg cggggccggg atcgcgggga tctccacggt
     3181 cgcgccgacg tcgttcaggt acttgacgaa cttgagcgcg tgcatgcgct cttcgtccgc
     3241 ctgcttgtga aacatgccgg ccagcttgcg gagcgccagc gactcgaagt agcaggcgat
     3301 gttcgtgtac tggtgcgagg cgaagaactc gcgcccgatc tcttcgttga tggccgcgtt
     3361 cagttccttg ctgatgagca tagattctcc tggattggga gagctgtcgt tctcgttccc
     3421 cggatgttcg tccatcatac cgcagacctc gggcagcccg tccgttctcg gacggcccgc
     3481 gcgccttgtc ggggccgctg cccttgcggc aagctgtgac gatggaaacg gtcgccctgc
     3541 cttccccgaa cgccgcagac caggcagcct tcctggccgc tcaccccgag tacgaggcga
     3601 cccgggggat cgacgagctg cgcgcgcgcg agttcgcccg gctcgacgcc cacggcgacg
     3661 tctacctgga ttacaccggg ggcgggctct acgccgcctc gcaggtgcgc gcgcacgccg
     3721 agcagctgct ggcgcacgtg ttcggcaacc cgcactcgtc gaaccccacg tccctgagat
     3781 cgtccgagca cgtggagcgc tgccgcgcgg acgtcctccg gtacttcaac gcgccgcggg
     3841 acgagtacac cgtcatcttc accaacaacg ccagccaggc cctgaaactg gtcggcgagt
     3901 cgtacccctt cgaaccgggc agcacccttc tcctcacctt cgacaaccac aactcggtga
     3961 acggcatccg ggagttcgcc cgaagccggg gcgcgcggac ggtgtacctg ccggtgttcc
     4021 cgcccgacat gcgggccgac cccgcccttg tcgccgggcg gctcgacgcg ctggcctccc
     4081 cggcgccgaa cctgttcgct taccccgccc agtcgaactt ctccggcgtg caacacccgc
     4141 tcgagtgggt ggcgcgggcg caggagcggg gctgggacgt gctgctcgac gccgccgcgt
     4201 tcgtcccggc caaccggctc gacctctccg ccgtgcagcc ggagtacctc gtcctgtcgt
     4261 tctacaagat gttcggctac ccgaccgggg tcggagcgct gctggcccgc cggcgcgcgc
     4321 tcgagaagct ccatcgcccg tggttcgccg gtggcacgat cacggtggca tcggtccagg
     4381 ccgaccgcca ctacctggca cccgggagcg cggcgttcga agaaggcagc ccgtcgtacc
     4441 tcaccattcc cgccgtggag ttcggcctgt cgctcgtcga gtcggtgggg atcgacacca
     4501 tccacacgcg cgtccgctgc ctggcgggct ggctcatcga cgccctgctc tcgctgctcc
     4561 acgacaacgg cgagccgctg gtgcgcttgt atggcccggc aaacacgacg gcgcggggcg
     4621 caaccctcgc gctgaatttc tacgaccgaa tcgggcgcgc cctggaccac cgcctcatcg
     4681 agcggcaggc gaaccgcgag cggatctcgc tgcgcacggg ctgcttctgc aatcccgggg
     4741 cgggcgaaac ggcgctcggc ttgaccaagg gcgagctgga ctcgtgcttc ctgcactcgt
     4801 cgcgccgcat gacgttcgat cagttccgca gctgcatcga cggcaggagc agcggggcgg
     4861 tgcgcgtttc gctggggctg gccagcaact tcgacgacgt cctcgccatg cgcaacttcg
     4921 cgcgccagtt cctcgactgg gagaacgggc cggaggacta gataggctca gggcgtttgg
     4981 cgaaggcggg gctcacggct ctgggcggag gcggtggagg gcgatgccgg tggcggtggc
     5041 gacgttgagc gaatcgacac cgggcgccat cgggatgcga acctgcaggg tggccgccgc
     5101 gagggccgcg ggggtcaggc ctgccccctc cgatcccgcc accagcacga agcgttcggg
     5161 cgggcgcctg gcgacgtcct cgataggcac cgcggacggg tcggtcgtca gcgcgatcac
     5221 ggcaaacccg cggcccgcga gcatctcgag acactgcggc caccccgtcg cctccacgaa
     5281 gggcacgcgc agcgccgcac ccatcgaaac cctgatcgtc ttgcggtaga gggggtcggc
     5341 gcacgacggc ccgaccagca gcgcgtcggc cccgaaggcg agcgcgttgc ggaacaggcc
     5401 gccgaggttg tcggggttgg cgacgccctc ggcgccaacg agcagccggg cgtcgggccg
     5461 ctccccgagc cactcgccga catcggccga agcggggcgc tcagcaatcg ccaggcaccc
     5521 gcggttgatg tcgtacccgg tcagcgccgc gaagatctcc cgcggggcca ggtagacgtc
     5581 gagatccttt agccggggga ccacctggtc ggcaagcgcg tcgagggcgg cctcgttcac
     5641 cagcagcgaa cgcacgcgga agcgcggcac cgcgagaagg acccgcacca cctgccgccc
     5701 ctcggcgacc agcagcccgt ctcgccgcag cctggccggc tccgcgacac cgcggtagtc
     5761 cgccagcctc gggtcggcgg cctcgtcaac ctggatcagc attcacctac tggtagatga
     5821 cgatcgaagg cgtgggcttc agctccagca cgtgcgacgg cgagagcagg tcgggttcct
     5881 tccggtagaa gagcttgatc cccgagtgct cgaggggggt ccgcgtgacc atccggtaga
     5941 tggcgtgctt cagctggcgc gggccccacc cgtccatcac cagggcaacg tccaccacgg
     6001 ggctgtcttg cacgttccgc ttgtcgggca gcatgttcat cgtgaactgg tgggcgatca
     6061 gcaccttggg cggcagcttc ttctcgatca cgattcgttc gagcaggtcg atggcgtagt
     6121 tcacgtcccg cgccggcgtc gtcccgatgc gcgtgccggg gggctcgccg ttgggcatgt
     6181 cgaactcggg gtcgagggcc aggtggacgt cgggctcttc caggtagcgg cgcaggtact
     6241 ccagctcggc ctcgacggtc gagtggccca cctgcacgtc gagcacgagc gcgaagccgt
     6301 gcgcccgcgc ctcggcgagc atccgctcga tcaccgcgcg cgactcgcgg cgccgccacg
     6361 tcccgtcgcg ccccgggttg ttctgcgcga cgacggcgac cagctcgtag gcgggctgga
     6421 cgcgcttgtc ggtcagcgcc gcgtaggcgt cggcctggcg ccgcaggccg ctcgccagct
     6481 cgtcaccggt gtgctcgccg agaatcccca tccgcttcga gtgcgggttg ccgtaccagg
     6541 tgacgagcag cgtgtgctgc agcacggact cggcaaccag ctcgatgtcg cccgtcttct
     6601 tctgcaccgg cacacctgtc tcgccgctcg ggggggcacc cggcagcggc ggg
//
LOCUS       DAOPQX010000200         6547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_12912_length_6547_cov_3.632009, whole genome shotgun sequence.
ACCESSION   DAOPQX010000200 DAOPQX010000000
VERSION     DAOPQX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12912_length_6547_cov_3.632009"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /gene="tsaD"
                     /locus_tag="PKK95_11055"
     CDS             <1..997
                     /gene="tsaD"
                     /locus_tag="PKK95_11055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOC18801.1"
                     /translation="IHREWGGVVPELASRQHVRDICGVVERALDEAGIALSGLDAVAV
                     TQGPGLVGSLLVGLSFAKSLAASIDRPLVPVHHLAGHIESLFLHNGPLPLPAAILVVS
                     GGHTSLYLVPEPGRYHLIGRTRDDAAGEAYDKVAKLLGLGYPGGPIIDRLARTGDDRA
                     VGFPSTRITHRDRNAPNLPGRFDFSFSGIKTAVLRHVRERTGTADSRQRPADSAPGSS
                     AAASVSTAFSEREIADICASFQRTVVEVLVDRLFEAAWRHRAKSVGIAGGVSANSRLR
                     QDAVRRGEQLGLPVYLPTLALSTDNAAMIAAAGLRRYALGVRAGLDLNADASLAL"
     gene            1063..1479
                     /locus_tag="PKK95_11060"
     CDS             1063..1479
                     /locus_tag="PKK95_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOC18802.1"
                     /translation="MKTYTDYLFFNTSQRREMVRITDEIAEIVRKSGVADGMVLVSAM
                     HITAGVYVNDWEEGLIEDFGEWLEKLAPSGRDYKHHRTGEDNGDAHLKRTIMGHQVIL
                     PITNGRLDLGPWEQVFYAEFDGRRRKRVVVKVMGAA"
     gene            complement(1512..3005)
                     /locus_tag="PKK95_11065"
     CDS             complement(1512..3005)
                     /locus_tag="PKK95_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC18803.1"
                     /translation="MASSPQSSSATTLDTEFKRGLGLYDSTMIVVGAMIGSGIFIVSA
                     DMARRLGSPGWLLVAWVVTGVLTITAALSYGELAAMMPRAGGQYVYLREAYSPLMGFL
                     YGWTLFMVIQTGTIAAVAVAFAKYLGVLVPWVSETRYIVPLVHLSEGYALSFSTAQLV
                     AVLLIVLLTWTNTRGLAYGRVIQNVFTSAKTVGLAFLIVVGLLLGWHVDAIAANFGSW
                     SAFWSPRGLQAVAPGLDATTVFGLFVAICVVQTGSLFSSDAWHIVTFTAGEVKNPRRN
                     LPLSLAIGTILVIGLYLLTNVAYLVMLPLEAIQTVPGDRVATAAFETVFPGLGVVLMA
                     VIIMVSTFGCDNGLILAGARTYYAMARDRLFFKAVGRLNEARVPAAALVLQAAWASLL
                     VLPRTYNPVTKQYGSLYSDLLDYVISAAVLFYILSISAIFRLRATRPDAERPYKALGY
                     PVVPALYILGASTMLAVLFVYRTSTTWPGLIIIALGIPVYFLWARKQ"
     gene            3071..3643
                     /locus_tag="PKK95_11070"
     CDS             3071..3643
                     /locus_tag="PKK95_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOC18804.1"
                     /translation="MSELSREAAWALLAEYTQNENLRKHALAVEAAVRFYARKFGEDE
                     EDWGAVALLHDFDYERYPTAEDHPFRGAEILRGLGYPEWAVRAILSHADYTGVTRESL
                     LERTLFACDEMAGFVTAAALVRPSKSVLDLEASSVVKRMKDKAFARAVSRDDLRKGAE
                     ALGLPLAEHIGNVIAGMREKAAELGLKGSL"
     gene            3722..4642
                     /locus_tag="PKK95_11075"
     CDS             3722..4642
                     /locus_tag="PKK95_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18805.1"
                     /translation="MPDPVVLLDNLSVSYGKNAALRDVSAAFLPGAVGLLGPNGAGKS
                     TLLKTLLGFLAPDRGSMRVLGLDVAKSALEIRARIGYMPETDSHIPGMNAVSFVGYCG
                     QLAGLPASDAVQRAHEVLYYVGLGEARYRNLETYSTGMKQRIKLAQALVHDPDLLFLD
                     EPTNGMDPRGREEMLELVRDLAHEKQVNLILSSHLLPDVEFTCDRVVVLDKGRVVAEG
                     PIDDLKGRRRQVFELRVKGDRHAFAAVLQREGFECHETDEDVMRVFVPGEGDGRSLFA
                     LAAREQVQVRHLRPSVPTLEDVFAKAVGEE"
     gene            4659..5498
                     /locus_tag="PKK95_11080"
     CDS             4659..5498
                     /locus_tag="PKK95_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18806.1"
                     /translation="MPIHDQSYRRYGGGREPIGKAWAVIARHGVQTIVRKRLFLGLLL
                     LAWIPFAIGAVSVYLAANFPQMSLLSMTAQRFRDFLAAQDFFVFVITVWVGAGLIAND
                     KRANALQIYLSKPLGRVEYVAGKLTILAVFLLGVTLLPALLLLILQVLFTGSFTFLRQ
                     NFYLFPAVIVFSFLQVLLASFTMLALSSISKSSRYVAILYAAVIFFSDAVYGLVYLIT
                     GSSRMSWLSLPANLRQIGDVVFRLEPRYATHPALSLLAVLAAMGLAAWILERQVRGVE
                     VVS"
     gene            5495..6430
                     /locus_tag="PKK95_11085"
     CDS             5495..6430
                     /locus_tag="PKK95_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18807.1"
                     /translation="MTQPAAVPPVVEAATLSKWYGQVIGLNDVTVTVPRGITGLLGPN
                     GAGKSTFMKLMTGQLKPSKGSIRLLGQPTWGNPSVYFKVGFCPDQDAFYDRMTGLEWV
                     TALVRLNGYGDRQAADAARRALGTVDLLDAADRKIGSYSKGMRQRVKLAQAIVHDPDV
                     LILDEPLSGMDPLGRRRTIRLIKEWARQGRSVIVSSHILHEIESMTSNILLINNGRIL
                     AEGNVHQIRDLIDTHPHTVFIRSADPRALARRFLAHDDVISLRFEEGAVVVQTASPDG
                     FYSRLTELAASGEAEIGEVTSPDDNLQAVFQYLVK"
BASE COUNT         1047 a         2218 c         2325 g          957 t
ORIGIN      
        1 gatccaccgc gagtggggcg gggtggtgcc cgagctggcc tcgcgccagc acgtgcgcga
       61 catctgcggg gtggtcgagc gcgcgctcga cgaggcgggc atcgccctgt ccggcctcga
      121 cgcggtggcc gtgacgcagg gccccggcct cgtcggctcg ctgctggtcg gcctctcgtt
      181 cgccaagtcg ctcgcggcct cgatcgaccg cccgctcgtg ccggtgcacc acctggcggg
      241 ccacatcgag tcgctcttcc tgcacaacgg gccgctgccg ctgcccgcgg ccatcctggt
      301 cgtgtcgggt gggcacacca gcctctacct ggtgccagag ccgggccgct accacctgat
      361 tggccgcaca cgggacgatg cggcgggcga ggcgtacgac aaggtcgcca agctgctggg
      421 cctcggctac cccggcgggc cgatcatcga caggctggcg cggacgggcg acgaccgggc
      481 ggtgggcttt ccctccacgc ggatcaccca tcgcgaccgc aacgcgccca atctaccggg
      541 ccggttcgac ttcagcttca gcgggatcaa gaccgcggtg ctgcggcacg tcagggagag
      601 gactgggaca gcggacagca gacagcggcc agcggacagc gcgccgggca gctcggcggc
      661 cgcttcggtg tccacggcct tttcggagcg ggagattgcc gacatctgcg cgagcttcca
      721 gcggacggtc gtcgaggtgc tggtggaccg cctgttcgag gcggcgtgga ggcaccgggc
      781 gaagagcgtg gggatcgcgg gcggcgtctc ggcgaacagc cggctccgcc aggacgcggt
      841 gcggcgcggc gagcagctcg gcctgccggt ctacctcccg acgctggcgc tctcgaccga
      901 caacgcggcg atgattgccg ccgcgggcct acggcgctat gcgctcggcg tgcgcgccgg
      961 cctcgacctc aacgccgacg cctcgctcgc gctctgatcc cagtggcaca ggcgtcccgc
     1021 ctgtgaggca gtcgccaccg ggaactggcc tacgatggat gcatgaagac ctacaccgat
     1081 tacctctttt tcaacaccag ccagcggcgc gagatggtgc ggatcaccga cgagatcgcc
     1141 gagatcgtcc gcaagagcgg cgtcgccgat ggcatggtgc tggtctcggc catgcacatc
     1201 accgccggcg tgtacgtcaa cgactgggag gaggggctga tcgaggactt cggcgagtgg
     1261 ctcgagaagc tggcaccgtc ggggcgcgac tacaagcacc accggaccgg cgaggacaac
     1321 ggcgacgccc acctgaagcg caccatcatg gggcaccagg tgatcctgcc catcaccaac
     1381 ggccggctcg acctgggccc ttgggagcag gtcttctacg ccgagttcga cggacggagg
     1441 aggaaaaggg tggtggtgaa ggtgatgggg gcggcctagc gtggcgggcg gccacaggcg
     1501 ggacgcctgt gctactgctt cctggcccag aggaagtaca ccgggatccc gagggcgatg
     1561 atgatgaggc cgggccaggt ggtggaggtg cggtagacga agaggacggc cagcatggtg
     1621 gaggcgccga ggatgtagag cgccgggacg acggggtacc cgagggcctt gtaagggcgt
     1681 tcggcgtcgg ggcgcgtggc gcgcaggcgg aagatggccg agatcgacag gatgtagaag
     1741 agcaccgccg ccgagatgac gtagtcgagc aggtcgctgt agaggctgcc gtactgcttc
     1801 gtcaccgggt tgtaggtgcg gggcagcacc aggagcgacg cccacgcggc ctgcagcacc
     1861 agcgcggcgg ccggcaccct ggcctcgttc agccgcccga ccgccttgaa gaacagccgg
     1921 tcgcgcgcca tcgcgtagta ggtgcgggcg ccggccagga tgaggccgtt gtcgcagccg
     1981 aaggtggaga ccatgatgat cacggccatc aacaccacgc cgaggcccgg gaacacggtc
     2041 tcgaaggcgg cggtcgccac ccggtcgccg ggcaccgtct ggatcgcctc gaggggcagc
     2101 atcaccaggt aggcgacgtt ggtcagcagg tacaggccga tgaccaggat ggtgccgatc
     2161 gccagggaaa gggggaggtt cctccgcggg ttcttcacct cgccggccgt gaaggtgacg
     2221 atgtgccagg cgtccgacga gaagagcgag ccggtctgca ccacgcagat ggcgacgaac
     2281 agcccgaaca cggtggtcgc gtcgaggccg ggcgccacgg cctgcagccc gcgcgggctc
     2341 cagaacgccg accagctgcc gaagttcgcg gcgatggcat cgacgtgcca cccgagcagc
     2401 agccccacca cgatcaggaa ggccaggccg accgtcttgg ccgaggtgaa cacgttctgg
     2461 atgacgcgcc cgtaggcgag gccgcgggtg ttcgtccacg tcagaaggac gatcagcagc
     2521 acggccacca gctgcgcggt ggagaacgac agcgcgtacc cctcggagag gtgcaccagc
     2581 ggcacgatgt agcgcgtctc cgagacccac gggaccagca cgcccaggta cttcgcgaaa
     2641 gcgacggcaa cggccgcgat ggttccggtc tggatcacca tgaacagcgt ccagccgtac
     2701 aggaagccca tgagcggcga gtaggcctcg cgcaggtaga cgtactggcc gccggcgcgc
     2761 ggcatcattg cggccagctc gccgtacgac agggccgcgg tgatggtcag cacgccggtc
     2821 accacccagg cgacgagcag ccagcccggg ctgcccaggc gcctcgccat gtcggccgag
     2881 acgatgaaga tgcccgagcc gatcatcgcc ccgacgacga tcatggtgga gtcgtagagg
     2941 ccgaggccgc gcttgaactc ggtgtcgagg gtggtggccg acgaggactg cggcgaactg
     3001 gccatgcgcg gctccctggg gcgggtacgg cgcggttgtg tcgcgcgtgg cgcgaatata
     3061 tcatgcgccc atgtcagagc tttcccgaga ggccgcctgg gccctgctgg cggaatacac
     3121 gcagaacgag aacctgcgga agcacgccct cgcggtcgag gcggccgtcc gcttctacgc
     3181 ccggaagttc ggcgaggacg aggaggactg gggcgcggtc gccctcctcc acgacttcga
     3241 ctacgagcgc tacccgacgg cggaggatca ccccttccgc ggcgccgaga tcctgcgggg
     3301 cctcggctac cccgagtggg ccgtgcgggc gatcctgtcg cacgccgatt acaccggcgt
     3361 cacccgcgag tcgctgctcg agcgcacgct gttcgcctgc gacgagatgg ccggcttcgt
     3421 gaccgcggcg gccctggttc gcccgagcaa gagcgtgctc gacctcgagg cgtcctcggt
     3481 ggtcaagcgg atgaaggaca aggcgttcgc gcgggccgtc agccgcgacg acctccggaa
     3541 gggggccgag gcgctgggcc tgccgctcgc cgagcacatc gggaacgtca tcgccggcat
     3601 gcgcgagaag gccgcggaac tggggctgaa ggggagcctg taacgcaacg gccggcgccg
     3661 ggcagaaccg gcggcctcgg ccgctcgtat ctaggtggca ggcgggttcc gagccgttct
     3721 tatgcccgat ccagtggtcc tgctcgacaa tctcagcgtc tcctacggga agaacgcggc
     3781 gcttcgcgac gtgagcgcgg ccttcctgcc cggggccgtc gggctgctgg ggccgaacgg
     3841 ggccggcaag agcaccctcc tcaagacgct gctcggcttc ctggcgcccg accggggcag
     3901 catgcgggtg ctcggcctcg acgtcgcgaa atcggcgctc gagatccgcg cccgcatcgg
     3961 gtacatgccc gagaccgaca gtcacatccc cggcatgaac gccgtctcgt ttgtcggcta
     4021 ctgcggccag ctggccggcc ttcccgccag cgacgcggtg cagcgggcgc acgaggtgct
     4081 ctactacgtc gggctgggcg aggcgcgcta ccgcaacctc gagacgtact cgacggggat
     4141 gaagcagcgc atcaagctgg cgcaggcgct cgtgcacgac cccgacctgc tcttcctcga
     4201 cgagcccacc aacggcatgg acccgcgcgg gcgcgaggag atgctggagc tggtgcgcga
     4261 cctcgcccac gagaagcagg tcaacctgat cctctcgtcg cacctgctgc ccgacgtcga
     4321 gttcacctgc gaccgggtcg tagtgctcga caaggggcgc gtcgtggccg aggggccgat
     4381 cgacgacctg aaggggcggc ggcgccaggt gttcgagctg cgggtgaagg gcgaccgcca
     4441 cgcgttcgcc gccgtgctgc agcgcgaggg gttcgagtgc catgaaaccg acgaggacgt
     4501 gatgcgggtg ttcgtcccgg gcgagggcga cggccgctcg ctgttcgccc tcgccgctcg
     4561 cgagcaggtg caggtgcgcc acctccgccc gagcgtcccg accctcgagg acgtgttcgc
     4621 caaggcggta ggggaggagt gaaggggcgt cagaacccat gcccatccac gatcagtcct
     4681 accggcggta cggggggggt cgcgagccca tcggcaaggc gtgggcggtc atcgcacgcc
     4741 acggcgtgca gacgatcgtc cgcaagcgcc tgttcctcgg cctgctgctg ctcgcctgga
     4801 tcccgttcgc gatcggggcg gtgtcggtct acctggcggc caacttcccc cagatgtcgc
     4861 tgctgtcgat gacggcccag cgcttccgcg atttcctcgc ggcgcaggac ttcttcgtct
     4921 tcgtcatcac ggtctgggtg ggggccgggc tgatcgcgaa cgacaagcgg gccaacgccc
     4981 tgcagatcta cctgtcgaag ccgctcggcc gggtggagta cgtcgccggc aagctcacga
     5041 tcctcgcggt gttcctgctc ggcgtgacgc tgcttcccgc gctcctcctg ctcatcctgc
     5101 aggtcctgtt caccgggagc ttcacgttcc tccgccagaa cttctacctg ttcccggcgg
     5161 tgatcgtctt ctcgttcctg caggtgctgc tcgcctcgtt cacgatgctc gccctctcgt
     5221 cgatcagcaa gagcagccgc tacgtggcca tcctctacgc ggcggtcatc ttcttcagcg
     5281 acgcggtcta cggcctggtc tacctgatca ccgggagcag ccggatgtcg tggctgtcgc
     5341 tccccgcgaa cctgcggcag atcggcgacg tggtcttccg cctggagccc cgctacgcca
     5401 cccacccggc cctctcgctg ctggcagtcc tcgccgcgat ggggctggcc gcctggatcc
     5461 tcgaacgcca ggtgagggga gtcgaggtgg tgtcgtgacg cagcctgctg ccgttccgcc
     5521 ggtcgtggag gccgccaccc tctcgaagtg gtacggccag gtcatcggcc tcaacgacgt
     5581 gaccgtcacc gttccccgcg ggatcacggg gctgctcggg ccgaacggcg ccggcaagtc
     5641 gaccttcatg aagctgatga cgggccagct gaagccgagc aaggggagca tccggctgct
     5701 cggccagccc acctggggaa acccgtcggt ctacttcaag gtcggcttct gccccgacca
     5761 ggacgccttc tacgaccgga tgaccggcct cgagtgggtg acggcgctgg tgcgcctcaa
     5821 cgggtacggc gatcgccagg ccgccgacgc cgcccggcgc gccctcggca cggtcgacct
     5881 gctcgacgcc gccgaccgga agatcggcag ctactcgaag ggcatgcgcc agcgcgtgaa
     5941 gctggcgcag gcgatcgtcc acgaccccga cgtgctgatc ctcgacgagc ccctgtcggg
     6001 catggacccg ctcgggcgcc gccgcacgat ccgcctgatc aaggaatggg cccgccaggg
     6061 gcgcagcgtc atcgtctcga gccacatcct gcacgagatc gagtcgatga cctcgaatat
     6121 cctcctgatc aacaacgggc ggatcctcgc cgaggggaac gtccaccaga ttcgcgacct
     6181 gatcgacacg catccccaca ccgtcttcat ccggtccgcc gacccccgcg cgctggccag
     6241 gcggttcctg gcgcacgacg acgtgatcag cctgcggttc gaggaggggg cggtggtggt
     6301 gcagacggcg tcgcccgacg gcttctactc caggctgacg gaactggcgg cgagcggcga
     6361 ggccgagatc ggcgaggtca cctcgcccga cgacaacctg caggcagtct tccagtacct
     6421 ggtgaagtaa ggtggacgac gatctcgacg ccggacggcg aagcgtggac ctgcccgttg
     6481 aagcgccagg cgcggacttg tccgccgaag cgcgaagcgc gcagccggac ttgtccgccg
     6541 aagcgcg
//
LOCUS       DAOPQX010000201         6492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13046_length_6492_cov_5.356377, whole genome shotgun sequence.
ACCESSION   DAOPQX010000201 DAOPQX010000000
VERSION     DAOPQX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13046_length_6492_cov_5.356377"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKK95_11090"
     CDS             <1..993
                     /locus_tag="PKK95_11090"
                     /inference="COORDINATES: protein motif:HMM:NF015387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOC18808.1"
                     /translation="KYRVLADSLSSAFGGAPRPIPAIIQAEDFDDGGEGGAYHDTTTG
                     NSGGAYRTTGVDIEKTADAAGGYNVGWISAGEWLKYSVNAASAGTYRFDARVASPGVG
                     GTFHLEANGVDVTGPMTIPDTGGWQSWRTVSANVVLAAGPQALRVVFDRNGASGAAGN
                     LNFIRLVLLSASTPYGGSARAVPGVVQAEDFDEGGEGVAYHDGSAGNSGGAYRATGVD
                     LQRTTDSGGGYNVGWMAAGEWLQYTVKAEAAATYTLRARVAADGPGGTFHVEAGGVDV
                     SGPIAIPDTGGWQTWTTVEARVRLRAGVQVLRLVLDANGPTGVFGNVNYLEVVR"
     gene            complement(1023..1874)
                     /locus_tag="PKK95_11095"
     CDS             complement(1023..1874)
                     /locus_tag="PKK95_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC18809.1"
                     /translation="MSPGAKGRILVVDDDRALRHALATLLADAGYTVDQANDGSGALA
                     SLARASPDLVLLDLGLPDMGGLEVLDQLRGLASPPRVVVVTADDTPGTVLKAVRGQVD
                     GYVTKPFAPGGIVDVIEDVMAKPPAAALPIEIVSARPEWVELLAPCSLDVANRILGFM
                     MTLEADLPEKVRESVGQAFQELLFNAIEWGGKLDPARKVRISCIRTRRMLLYRIADPG
                     EGFDISRLSHAAINNPEDNPLQHALVREEKGLRPGGLGLAITRMLVDELVYNEKHNEV
                     VFVKYLD"
     gene            1967..4939
                     /locus_tag="PKK95_11100"
     CDS             1967..4939
                     /locus_tag="PKK95_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC18810.1"
                     /translation="MGAQIERRAANRAGAQQVRLVYETARALAESSTLAEAAPRMLES
                     VCQALAWEHGAFWSVDPLANVLRCVATWHQAAAGLDDFAAASEASVFAPGIGLPGRVW
                     ATEQAAWIPDVGQDSNFPRAGLADRAGLHAALGFPILADGKVIGVVEFFSRAIREPDE
                     ALLGTLKAVGSQVGLFVDRKRVEEELDRFFNLSLDLFCIASLDGMFLRLNPVWERVLG
                     FPRRELLAKPWLEFVHPDDLEATRGAGSRLLADMEVLDFENRYRCADGSYRWLQWTSA
                     PVRHLGLIYAVARDVTDRKRDEEALRRYAREMAAARREHEENAERLTQLVKELEVAKA
                     RAEEATVAKGEFLANMSHEIRTPMNAIIGMTDLALGTNLTDEQRDYLRTVKDSGEALL
                     ALVNDILDFSKIEARRMALDRVAFDLRDTVEDAVRLLAPRGHEKGLEVGCRIQPDVPH
                     QVVGDPGRLRQVVVNLVGNAIKFTDRGEVVVDVAAERQDADEAVLKFTVSDTGIGIPA
                     GKQWQIFGPFVQADASTTRRYGGTGLGLAISSQLVELMGGRIWIESEVGRGSRFHFLA
                     RFGMVEGTSVPSPPAGSAGLERMRVLVVDDNATNRRILEEMLASWRLEPETADGAEAA
                     LTRLRAAHEAGRPFRLVLLDALMPGTDGFMLAQAIKADARFSGISLIMLTSAALSDVQ
                     RRARRAGVAACLSKPVKQSDLFDAILATIAPAPRVQRGEAAAARGGAARRRGGGRVLV
                     AEDNPTNQKLVVLLLEQQGYQVVLAANGEEAVARSAEQPFDLILMDVQMPRLSGIEAT
                     VAIRERERGTGVHVPIVALTAHAMSGDRERCLAAGMDGYVAKPLRSNELFAAIDALLG
                     PSSSPGTPATPEAAAPEAGPLDAASLLAGFGGNRTLLGEVIDMFLADTPRLLSEARGA
                     LERGDAAALAASAHALKGSLGLFAQTGGAYEAARSVELAARAGDLPGSAGRFPELEEA
                     ARQLSEEVQAIRRSLQEP"
     gene            5038..5394
                     /locus_tag="PKK95_11105"
     CDS             5038..5394
                     /locus_tag="PKK95_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC18811.1"
                     /translation="MQRQPSRLELLQGTLELIILRTLLAGPAHGHAIAKHIQRTSEDL
                     LQVETGSLYPALHRLEAKGWVAASWEKSDKGKRARYYRLTALGRRQLAAEHSKWEAFS
                     RAMGLILKPAAQEEGR"
     gene            5391..>6492
                     /locus_tag="PKK95_11110"
     CDS             5391..>6492
                     /locus_tag="PKK95_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18812.1"
                     /translation="MRPFLRKLGWLIRRRDKEAELSEELQFHLDEEADERRAEGLTDD
                     EARRAARRALGNLTLVREDTRAAWGWTILEQLGQDVRYALRTMAANRLFTVLIVLSLG
                     LGIGANTAIYSFVDAILLRSLPVTDPESLVVLNWHAKGERVPVMRSMSGSTWGDSSAG
                     TTAGVFPFPVFELLRTKETVFSSVFAHFRYWQTRSVNVTIGGEADLAGGWHVSGGYFG
                     GLGVRPAAGRLIGDSDDKPGAPAVAVVSYAFSQRRFGGPAGAAGASILIDNLPFTVVG
                     VTPPGFFGADPAANPDVYLPLHALEYLGAGKQFGFDPSAYGAEHYYWIQIMGRLRPGV
                     TRAQAQAALAPAFHQWVAGTATNDPERANLPAL"
BASE COUNT          966 a         2219 c         2370 g          937 t
ORIGIN      
        1 aagtaccgcg tgctggccga ttcgctgtcg tcggccttcg gcggcgcgcc gcgcccgatc
       61 cccgccatca tccaggccga ggattttgac gatggggggg aagggggcgc gtatcacgac
      121 acgacgacgg gcaatagcgg cggcgcctac cggacgaccg gcgtggacat cgagaagacg
      181 gccgacgcgg cgggcggtta caacgtgggg tggatttcgg ccggcgagtg gctcaagtac
      241 tcggtgaacg cggcaagcgc cggcacgtac cggttcgatg cgagggttgc gtcgcccggt
      301 gtcggcggca ccttccacct cgaggcgaac ggcgtcgacg tgaccgggcc gatgacgatc
      361 cccgacaccg gcggctggca atcgtggcgc acggtctcgg cgaacgtcgt gctcgccgcc
      421 ggaccgcagg cgctgcgcgt ggtgttcgat cggaacggcg cgagcggcgc ggccgggaac
      481 ctcaacttca tccgcctggt cctcctgtcg gcatcgaccc cttacggcgg cagcgcgcgg
      541 gccgtgcccg gggtcgtcca ggccgaggac ttcgacgagg ggggcgaggg cgtcgcctac
      601 cacgacggat cggcgggcaa cagcggaggc gcctaccggg cgacgggcgt cgatcttcag
      661 aggactaccg acagtggggg cggctacaac gtgggctgga tggcggccgg cgagtggctg
      721 cagtacacgg tgaaggccga ggcggctgcc acctacacgc tgcgggcccg cgtcgcggct
      781 gacggtccgg gcgggacgtt ccacgtggag gcgggcggcg tggacgtttc cgggccgatc
      841 gcgatccccg acacgggagg ctggcagacc tggaccaccg tggaggcgcg ggttcgcctg
      901 cgcgccgggg tccaggtgct gcgcctcgtg ctcgacgcca acggcccgac gggcgtgttc
      961 ggcaacgtca actacctgga ggtcgtgagg tagaagcggc cgctcgcgcg gggcggccgc
     1021 aatcagtcga gatacttcac gaacacgacc tcgttgtgct tctcgttgta gacgagctcg
     1081 tcgaccagca ttcgcgtgat cgccaggccg agcccgcccg gccgcagccc cttttcctcg
     1141 cgcaccagcg cgtgctgcag cggattgtcc tcggggttgt tgatcgccgc gtgtgacagc
     1201 cggctgatgt cgaagccctc gccggggtcg gcgatccggt acaacagcat gcggcgcgtg
     1261 cggatgcacg agatgcgcac cttgcgcgcc ggatcgagct tcccgcccca ctcgatggcg
     1321 ttgaagagca attcctggaa cgcctgcccc accgactcgc gcaccttctc cggcaggtcg
     1381 gcctcgagcg tcatcatgaa ccccaggatg cggttcgcga cgtcgagcga acagggcgcc
     1441 agcagctcca cccactcggg gcgcgccgag acgatctcga tcggcagcgc cgcggccggg
     1501 ggcttcgcca tgacgtcctc gatcacgtcc acgatgccgc ccggggcaaa cggcttggtt
     1561 acgtagccgt ccacctggcc cctgacggcc ttgagcaccg tgccgggcgt gtcgtccgcg
     1621 gtcaccacga ccacgcgggg cggcgacgcg agcccgcgca gctgatcgag cacttcgagg
     1681 ccgcccatgt cgggcagccc gaggtcgagc agcaccaggt cgggggaggc gcgggcgagg
     1741 ctggccagcg cccccgaacc atcgttcgcc tggtcgacgg tgtaccccgc atcggcgagg
     1801 agggtcgcca gcgcgtgccg aagcgcccgg tcgtcgtcta cgacgagaat gcggcctttc
     1861 gcgccaggac tcatcggatc acctccagcc gaaggcggcg cggcgatcga agcaggcggg
     1921 attcgttgta gtgtactact catcccgcca ggaggaggtg cgaacgatgg gcgcgcagat
     1981 cgagcggcgc gcggccaacc gcgccggggc gcagcaggtc cgcctcgtct acgagaccgc
     2041 ccgcgcgctg gccgagtcgt ccacactggc cgaggcggcg ccgcgcatgc tcgagtcggt
     2101 gtgccaggcc ctggcgtggg aacacggggc gttctggagc gttgacccgt tggcgaacgt
     2161 gctgcgctgc gtggcgacct ggcaccaggc cgcggccggg ctggacgact tcgcggcggc
     2221 cagtgaggcc agcgtgttcg cccccggcat cggccttccg ggacgcgtct gggccaccga
     2281 gcaggcggcg tggatcccgg acgtcggcca ggattccaac ttccccaggg cgggcttggc
     2341 cgatcgcgcc ggcctccacg ccgcgctggg gttccccatc ctcgccgacg gcaaggtgat
     2401 cggcgtggtg gagttcttca gccgggcgat ccgcgagccc gacgaggcgc tgctgggcac
     2461 gctgaaggcg gtcggcagcc aggtcggcct tttcgtcgac cgcaagcgcg tcgaggaaga
     2521 gctggaccgc ttcttcaatc tctcgctcga cctcttctgc attgcgagcc tcgatggcat
     2581 gttcctccgc ctgaacccgg tctgggagcg ggtgctcggc ttcccgcggc gcgaactgct
     2641 ggccaagccc tggctcgagt tcgtgcaccc cgacgacctc gaggccacgc gcggggcggg
     2701 atccaggctg ctcgcggaca tggaggtgct cgacttcgag aaccgctacc ggtgcgccga
     2761 cggctcctac cggtggctcc agtggacgtc cgccccggtc aggcacctgg ggctcatcta
     2821 cgccgtggcg cgcgacgtga ccgatcgcaa gcgcgacgag gaagcgctgc ggcgctacgc
     2881 gcgcgagatg gcggcggccc ggcgcgagca cgaggagaat gcggagcggc tcacccagct
     2941 ggtcaaggaa ctcgaggtgg ccaaggcgcg cgccgaggag gcgaccgtcg ccaagggaga
     3001 gttcctggcg aacatgagcc acgagatccg cacgccgatg aacgcgatca tcgggatgac
     3061 cgaccttgcc ctcgggacga atctgaccga cgagcagcgc gactacctgc ggacggtgaa
     3121 ggactcgggc gaggcgctgc tggcgctcgt caacgacatc ctcgatttct cgaagatcga
     3181 agccaggcgg atggcgctcg atcgcgtggc gttcgacctg cgcgacacgg tcgaggacgc
     3241 cgtgcggctg ctcgccccga ggggccacga gaaggggctg gaagtcggct gccgcatcca
     3301 gcccgacgtc ccgcaccagg tcgtcggcga tccgggccgc ctccgccagg tcgtcgtgaa
     3361 cctcgtggga aacgcgatca agttcaccga tcgcggcgag gtggtcgtgg acgtggcggc
     3421 ggagcggcag gacgccgacg aggccgtgct gaagttcacg gtgtcggaca ccggcatcgg
     3481 catccctgcg gggaagcagt ggcagatctt cggccccttc gtgcaggccg atgcctcgac
     3541 gacgcgccgg tacgggggaa ccgggcttgg cctggccatc tcgtctcagc tcgtggagct
     3601 gatgggcgga cgcatctgga tcgagagcga ggtcgggcgc ggcagccggt tccacttcct
     3661 cgcacggttc gggatggtgg aaggaacgtc ggtgccctcc ccgccggcgg ggtccgccgg
     3721 cctcgagcgc atgcgggtgc tcgtcgtcga cgacaacgcg accaaccgcc gcatcctcga
     3781 ggagatgctc gcgagctggc ggctggaacc cgagaccgcc gatggagccg aggccgcgct
     3841 gacccggctg cgggccgcgc acgaggccgg ccgtcccttc cgcctcgtgc tgctggatgc
     3901 gctcatgccc ggcaccgacg ggttcatgct ggcccaggcg atcaaggccg acgcgcggtt
     3961 ctccggaatc agcctcatca tgctgacgtc ggccgcgctt tccgacgtgc agcggcgcgc
     4021 gcggcgggcc ggcgtggccg cctgcctcag caagcccgtc aagcagtcgg atctcttcga
     4081 cgcgatcctc gcgacgatcg ccccggcccc cagagtgcag cgcggggagg cggcagcggc
     4141 tcggggcggg gcggcgcggc gccgcggagg gggccgcgtg ctcgtggcgg aggacaatcc
     4201 caccaatcag aagctcgtcg tcctgctgct ggagcagcag ggataccagg tggtgctggc
     4261 cgccaacggg gaagaggcgg tggcccgttc ggccgaacag ccgttcgacc tgatcctgat
     4321 ggacgtccag atgccccggt tgagcggcat cgaggcaacc gtcgcgattc gcgagcggga
     4381 gcgcggcacc ggcgtccacg tgccgatcgt cgccctgacg gcgcacgcca tgtcgggcga
     4441 tcgggagcgg tgcctggcgg ccggcatgga cggctacgtc gccaagccgc tgcgatcgaa
     4501 cgagctgttc gcggccatcg acgcgttgct cgggccgtcg agcagccccg ggacgcccgc
     4561 tactccggag gcggccgcac ccgaggccgg gcccctcgat gccgcctcgc tgctggcggg
     4621 gttcggcggc aaccgcaccc tgctcggcga ggtgatcgac atgttcctcg ccgacacccc
     4681 gaggctcctg tccgaggcgc ggggcgcgct cgaacggggc gacgccgcgg cactggcggc
     4741 ctcggcccac gcgctgaaag gatcgctggg gctcttcgcg cagaccggcg gcgcctacga
     4801 ggcggcccgc tcggtcgagt tggccgcgcg cgccggcgac ctgccgggaa gcgccggccg
     4861 cttcccggaa ctggaagagg cggcgaggca gctgagcgag gaggtccagg ccattcgccg
     4921 gtcgttgcag gagccgtgag gcgcccacct ctgcacagcc tcgggcgacc acaagggtcg
     4981 cccctacggc cggtttgtgt agactgtcta ttcaatattg agtagactgc ctacactatg
     5041 cagcggcagc cgtcacgcct ggagctgctc cagggcacgc tcgagctcat catccttcgc
     5101 accctcctgg cgggcccggc gcacggtcac gccatcgcga aacacatcca gcggacctcg
     5161 gaagacctgc tccaggtcga gacgggttcc ctgtacccgg cgctccaccg cctggaagcg
     5221 aagggctggg ttgcagcgtc ctgggagaag tccgacaagg gcaagcgcgc aaggtactac
     5281 cgcctgacgg cgctcgggcg gcgtcagctg gcggccgagc actcgaagtg ggaggcgttc
     5341 agccgcgcca tgggcctgat cctgaaaccg gccgcgcagg aggaagggcg gtgagaccgt
     5401 tcttgcggaa gctgggctgg ctcatccgcc ggcgcgacaa ggaagccgag ctgagcgagg
     5461 agctgcagtt ccacctcgat gaggaagcgg acgagcgccg cgccgagggc ctgaccgacg
     5521 acgaggcgcg gcgggcggca cggcgtgcgc tgggcaacct cacgctcgtg cgcgaagaca
     5581 cccgcgccgc ctggggatgg acgatcctgg agcagctcgg ccaggacgtg cgctacgcgc
     5641 tgcggacgat ggccgccaac cggctgttca cggtgctgat cgtcctgtcg ctcgggctcg
     5701 gcatcggggc caacacggcg atctacagct tcgtggacgc catcctgttg cgctcgctcc
     5761 ccgtcacgga tcccgagtcc ctcgtcgttt tgaactggca cgcgaagggc gagcgagtcc
     5821 ccgtcatgcg ctcgatgagc ggcagcacgt ggggcgactc gtcggcgggt acgacggccg
     5881 gtgtcttccc gttcccggtc ttcgaactcc tccgcacgaa ggagactgtc ttctcgagcg
     5941 tgttcgcgca cttccggtac tggcagacca ggagcgtgaa cgtcacgatc ggcggagaag
     6001 ccgacctggc gggcggctgg cacgtgtcgg gcgggtactt cggcgggctc ggcgtccgcc
     6061 ccgcggccgg acggctgatt ggggacagcg acgacaagcc cggtgcgccc gcggtcgcgg
     6121 tcgtgagcta cgccttctcc cagcgccgct tcgggggccc ggcgggtgcc gccggcgcgt
     6181 cgatcctgat cgacaacctc ccgttcacgg tcgtcggtgt cacgccgccc gggttcttcg
     6241 gggcggatcc cgcggccaac cccgacgtct acctgccgtt gcacgcgctg gagtacctgg
     6301 gcgccggcaa gcagttcggc ttcgatccgt cggcctacgg ggccgagcac tactactgga
     6361 tccagatcat gggccgcctc cgcccgggag tgacgcgcgc ccaggcacag gcggcgctgg
     6421 ccccggcgtt tcatcaatgg gtcgccggca ccgcaacgaa cgatcccgaa cgcgcgaacc
     6481 tgcccgcgct cg
//
LOCUS       DAOPQX010000202         6421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13225_length_6421_cov_5.100534, whole genome shotgun sequence.
ACCESSION   DAOPQX010000202 DAOPQX010000000
VERSION     DAOPQX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13225_length_6421_cov_5.100534"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="PKK95_11115"
     CDS             <1..1936
                     /locus_tag="PKK95_11115"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOC18813.1"
                     /translation="ETSSWPLIAKSKAGFISQMGELSGLALAGAGKKLELVPYAVTQA
                     RSEPVDAGDPFRQSPDGSGTFGLDLKYALTPGLTLTATLNPDFGQVEADPAVVNLSAF
                     ETFYQERRPFFVEGSGNLQFNLDCNDGRCTGLFYSRRIGRQPHGGPDAPEGGYVATPT
                     LTTILGAAKLTGRIGSFAVGALNAVTGEESARLASPASQFRETVEPLTNFTVVQAKRQ
                     WSDQSSLGFAFTNTARRLDGDVGYLPSAATTGGVNWDWRLGSRYSFTGYWAGSAVRGS
                     ARAIDELQQNAVHYFQRPDAGHLEYDPMRTSLFGHAGSVAFQKIGGSKVRFSFNGAYK
                     TPGFEVNDVGYQRRADDIMQSGWVQVRWDTPTRLYRNVRLNFNQWAAWNFDGDSRFKG
                     GNVNAHILLHSNWSAGGGFNVEAAGIDDRATRGGPGLRSKNGANLWYYVGSDDRKAVS
                     GTWMSFMYRDEARNWVWGFDPEIRYRPTSFLTLSGGIHFDRVNDEGQWVENVGEGPST
                     AYVFGKIRQTTVGLTARCNYTITPNLTLQLYAQPFVSAGAYSDFKHVVKPRAPRYADQ
                     FAPYPYQGEPDFNYRSFRTTNVLRWEYRPGSALYVVWQQQREDSLSTGAMRFRRDLRE
                     LFGLPGTNVFLVKFSYWLNL"
     gene            2208..2435
                     /locus_tag="PKK95_11120"
     CDS             2208..2435
                     /locus_tag="PKK95_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18814.1"
                     /translation="MSGKEDVQVEQDEKGWAVKQGAKRLKRIAEGRERDGLQRACKWA
                     WEYASEHGVTAWLRLQGGNRRLRINFSKTRD"
     gene            2541..2948
                     /locus_tag="PKK95_11125"
     CDS             2541..2948
                     /locus_tag="PKK95_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC18815.1"
                     /translation="MATAQAAVQIDAIVPTLTVDDLQKSIAFYEAIGFTIDDRWEHEG
                     TLLGVMMRAGRNQIGLNQDDWKKGRDRKKGTGVRLSMSVSTPENVDAIARRAKSAGLP
                     LKSEPQDTEWDSRAFELVDPTGYLLTVYSEAPA"
     gene            3031..3804
                     /locus_tag="PKK95_11130"
     CDS             3031..3804
                     /locus_tag="PKK95_11130"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC18816.1"
                     /translation="MEVQSHAHGTFCFPELNTQDIARDKRFYSELLGWSAFDVPSAAG
                     GYSLMQLRGKDVAGVHLARNGPFGWLSYISVDSADRTASLARTLGGAVIADPFDVPGI
                     GRMSMLQDPAGARFALWQAAGHSGVRLADEPGAMYWNELFAHDVAAARTFYSGLFGWT
                     TRETEVPSGPYTIFDAGERSAAGMIQIQKDWGPVPPHWEVYFAVGDCDGAMRRAVELG
                     GTVLYGPLDVPKAGRLAGLQDLGRASFTVMQPAHAHEEA"
     gene            3935..5599
                     /locus_tag="PKK95_11135"
     CDS             3935..5599
                     /locus_tag="PKK95_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOC18817.1"
                     /translation="MRRLATRVSLHRVLVAAAAAAVAAATLLAAVAAAARQEAGPDSP
                     GSVALLAGKTDAASIEALARAVRSGAPLVRAVGARAAGVVAAASLGDAVAAAFDREGD
                     PTAAVEQARALLFLRGEAATGSIERRLASVPDVAIAYGEWLVRMQPERFPDLLERLPA
                     QAVPSLEPAVKGSLARRPEFAERVLRAWLAARPAAPWIRVLDGVDLASPGITAAIAHA
                     LGVQDEPLREDTVWMLVDRLAEGLVVPGPLLEAAAAPLPGAAPADSGTVTWERFGREL
                     VARRSGVKTPDRAALIKSEGRGRQRQLQVLWLLDLLSKGERAEVKRLLDNPPPGRAAS
                     GPPASADETGVWMRTIPAAWPGVVGAVMEAAGCSPGPGANAAVLSVTYRPDGRTARLT
                     LQDTRLPAPCGAAASSLAALTLHDLEHGPPAGQVEMLVLPLDPDYIACASASESVRSR
                     HVPSQAGSDASSSSGKVKAPKKTRHVNPVYPAAMQELKVQGTVWVEATITPAGCVAAG
                     RLVKSPHLLLGLSSLWAVSGWRFEPTLLAGKPVPVIISVAVDYKLR"
     gene            complement(5824..6195)
                     /locus_tag="PKK95_11140"
     CDS             complement(5824..6195)
                     /locus_tag="PKK95_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18818.1"
                     /translation="MAARILFHGDIMMRALLEAYWERDPEMRLYQRMVERFGAEPRAG
                     ASTDSRDCLRLVRSMTREQWRHTQQRLAEEWRRQLEERRKNAAPPTPEPTGRYSWGRR
                     YKAELEARQREAEREKREGSK"
BASE COUNT          970 a         2191 c         2266 g          994 t
ORIGIN      
        1 cgagacctcg tcgtggccgc tcatcgccaa gagcaaggcg gggttcatct cgcagatggg
       61 cgagctgtcc ggtctcgcgc tcgcgggtgc cgggaagaag ctcgagctgg tgccgtacgc
      121 cgtgacgcag gcccgctcgg agccggtcga cgcgggggac ccgttccggc agtcgcccga
      181 cgggtccggt accttcggcc tcgacctgaa gtacgcgctc acgccgggcc tcacgctcac
      241 cgccacgctg aaccccgact tcggccaggt cgaggccgac ccggccgtcg tcaacctcag
      301 cgcgttcgag accttctacc aggagcgccg gcccttcttc gtcgagggat cgggcaacct
      361 gcagttcaat ctcgactgca acgacggccg ctgcaccggg ctcttctact cgcggcgcat
      421 cggccgccag ccgcacgggg ggcccgacgc gcccgagggc gggtacgtgg ccacccccac
      481 gctcaccacg atcctcggtg ccgccaaact gacggggcgc atcggcagct tcgccgtcgg
      541 cgccctcaac gccgtcaccg gagaggagtc ggcgcgcctg gccagccctg cctcgcagtt
      601 ccgcgaaacc gtggagccgc tgaccaactt caccgtcgtg caggcgaagc ggcagtggag
      661 cgatcagtcc tctttgggct ttgcgttcac gaacaccgcc cgccggctcg acggcgacgt
      721 cggctacctg ccgtcggcgg ccacgacggg cggggtgaac tgggactggc ggctgggatc
      781 gcggtactcg ttcacggggt actgggcggg gagcgccgtg cgggggagtg cgcgggcgat
      841 cgacgagctg cagcagaacg ccgtccacta cttccagagg cccgacgccg ggcacctgga
      901 gtacgacccg atgcggacct cgctgttcgg gcacgccggc tcggtggcgt tccagaagat
      961 cggcgggtcg aaggtgcgct tcagcttcaa cggcgcctac aagacgcccg ggttcgaggt
     1021 gaacgacgtc ggctaccagc gccgggccga cgacatcatg cagtcgggct gggtgcaggt
     1081 tcgctgggac acccccacca ggctctaccg gaacgtccgg ctgaacttca accagtgggc
     1141 tgcgtggaac ttcgacggcg actctcgctt caagggcggc aacgtcaacg cgcacatcct
     1201 gctgcactcg aactggtcgg ccggcggcgg gttcaacgtc gaggccgcgg ggatcgacga
     1261 ccgggccacc cgcggcgggc cgggcctgcg ctcgaagaac ggcgcaaacc tctggtatta
     1321 cgtcggcagc gacgaccgga aggcggtgtc gggcacctgg atgtcgttca tgtaccggga
     1381 cgaggcacgc aactgggtgt ggggcttcga ccccgagatc aggtaccggc cgacctcgtt
     1441 cctcaccctg tcgggcggaa tccacttcga tcgggtgaac gacgaagggc agtgggtgga
     1501 gaacgtgggg gaggggccgt caaccgcgta cgtgttcggc aagatcaggc agacgaccgt
     1561 cgggctgacg gcgcgctgca actacaccat cacgcccaac ctcaccctgc agctgtacgc
     1621 gcagccgttc gtgtcggccg gcgcctactc cgacttcaag cacgtggtga agccccgcgc
     1681 gccgcggtac gccgaccagt tcgcgccgta cccctaccag ggtgagcccg acttcaacta
     1741 ccgctcgttc cgcaccacca acgtgctgcg ctgggaatac cgtcccggct cggcgctcta
     1801 cgtggtgtgg cagcagcagc gcgaggattc gctctccacg ggggcgatgc gttttcggcg
     1861 cgacctgcgc gagctgttcg gccttcccgg gaccaacgtc ttcctcgtga agttctccta
     1921 ctggctgaac ctgtagctca cggtctggct caccgtcgtc gagcgggcgc ggcgccttgc
     1981 acaagacctc gagcgggtgc ggcgcttacc aaagacgtca ggcctcacga attgcgtcgg
     2041 ccggcaccgg agcggcgccg gacgccgggc ttgcccgccc gcccaccgcg ctcccgcgcg
     2101 cacgtctttt cgcaaggcga acgcgtccgc atggccggga cccgcggtcg aacgcggccg
     2161 gagcgctgga tggaacccga tttggacgcc tgctcgccga ggaccgagtg agcggaaaag
     2221 aagacgtcca ggtggagcag gacgaaaagg gctgggccgt gaaacagggc gcgaaacgac
     2281 tcaagcggat cgccgagggg cgcgagagag acggcctgca gcgggcgtgc aagtgggcgt
     2341 gggagtacgc ctccgagcac ggcgtcaccg cctggcttcg gctgcagggc ggcaatcgcc
     2401 ggttgaggat caacttcagc aagacacgcg actgacggtc gccgggccgc tgcatggcgc
     2461 ggccgagccg cgcggccgcg cttgcagatc cgggcgccgg cggctagcct gcaagtgaac
     2521 cccaccacct ggaggccctc atggcgacag cgcaggcagc cgtgcagatc gacgcgatcg
     2581 ttcccaccct caccgtcgac gacctgcaga agagcatcgc gttctacgag gcgattggtt
     2641 tcacgatcga cgaccgctgg gagcacgagg ggacgttgct cggcgtgatg atgcgcgccg
     2701 gcaggaatca gatcggcttg aaccaggacg actggaagaa ggggcgcgac cgcaagaagg
     2761 ggaccggcgt gcggctctcc atgtcggtct cgacgccgga gaacgtcgat gcgatcgcga
     2821 ggcgcgcgaa gagcgccggc ctcccgctga agtcggagcc gcaggacacc gagtgggaca
     2881 gccgggcgtt cgagctcgtc gatccgaccg gctacctgct gacggtgtat tcggaggccc
     2941 cggcgtaggc gcgcggattc ctgcggaaga acggcagtag atcccgaggt tcgcgagcga
     3001 accggctacc atggagccga ggaggccccc atggaagtcc agtcgcacgc gcacggaacc
     3061 ttctgctttc cagagctgaa cacgcaggac atcgctcggg acaagcgctt ctactcggaa
     3121 ctgctcgggt ggagcgcctt cgacgtgccg agcgcagccg gcggctattc cttgatgcag
     3181 ttgcgcggca aggacgtcgc cggggtgcat ctggcccgga acggcccctt cggttggctc
     3241 tcctacatct ccgtcgacag tgccgaccgg accgcatcgc tggcgcggac gctcggtgga
     3301 gccgtcatcg ccgacccatt cgatgtgccg ggtatagggc gaatgtcgat gctccaggat
     3361 ccggcgggtg ccaggttcgc tctctggcag gcggcgggac actcgggggt gcggctggcg
     3421 gacgagcccg gagcgatgta ctggaacgag ctgttcgccc acgatgtcgc ggccgcaagg
     3481 accttctatt cgggcctctt tggatggacg acacgcgaga cggaggttcc atccggtccg
     3541 tacacaatct tcgacgccgg cgagcgttcg gctgccggca tgattcagat tcagaaggac
     3601 tggggtcccg taccgccgca ttgggaggtc tatttcgcgg tcggggactg cgacggcgcc
     3661 atgaggcgcg ccgtcgagtt gggtggcacg gtcctgtacg gtccgctcga cgtgccaaag
     3721 gccggacggc ttgcgggact ccaggacctc ggccgagcct cgttcacggt gatgcagccg
     3781 gcgcatgccc acgaggaggc gtgacgcgga agaacatata gacctcccgg gagcgtgcga
     3841 cctcgcggtc cggttctcct gggaggaggt gagggtttcg caggaaaagg ttacggtcgc
     3901 acgcgttggc gcacgtctgc taagctccgc cgttgtgaga cgtctcgcca cccgtgtttc
     3961 cctgcaccgt gtcctggttg ccgcggctgc cgcagccgtc gcggcggcca ctctccttgc
     4021 tgcagtcgcg gctgcggcgc ggcaggaggc cggacctgat tcacctggtt ccgtcgcgct
     4081 tctcgccggc aagacggacg ccgcgagcat cgaggcgctc gcgcgtgccg tcagatcggg
     4141 cgcaccgctc gtccgcgcgg tcggcgcgcg cgcggcaggc gtggtcgccg cggcgtcgct
     4201 gggggacgcc gtcgccgccg ccttcgatcg ggaaggggat cccacggcgg cggtcgagca
     4261 ggcgcgggcc ctcctgttcc tgcgcggcga ggccgcgacc gggtcgatcg agcggcgcct
     4321 tgcctccgtg cccgatgtcg cgatcgccta cggcgagtgg ctcgtgcgca tgcagcccga
     4381 gcggttcccc gacctgctcg agaggctgcc tgcgcaagcc gtcccctcgc tcgagccggc
     4441 cgtgaagggg tcgctggccc gccggccgga attcgccgag cgcgtgctgc gggcctggct
     4501 ggctgcgcgc cccgcagcgc cttggatccg ggtcctggat ggagtggatc tcgcttcacc
     4561 cggcatcacg gccgcgatcg cgcatgcgct gggcgtgcag gacgagcccc tccgcgagga
     4621 cacggtctgg atgctggtcg accggctcgc ggagggcctt gtcgttccgg gcccgctgct
     4681 ggaggccgcc gccgccccgc tccccggcgc cgcgccagcc gattccggca cggtgacgtg
     4741 ggaacgcttc gggcgcgagc tcgttgcccg gcggagcggg gtgaagacgc cggaccgtgc
     4801 cgcgctcatc aagtcggagg ggcgcggccg ccagcggcag cttcaggtgc tgtggctcct
     4861 ggacttgctg tcgaagggcg agcgcgccga ggtgaagcgc cttctcgaca atccgccgcc
     4921 gggacgtgca gcgtccggcc ccccggcaag cgcagacgag accggcgtgt ggatgcggac
     4981 gattcccgcc gcctggcccg gtgttgtcgg ggcagtgatg gaggcggccg gctgctcgcc
     5041 cgggccgggc gcgaatgctg ccgtcctgtc ggtcacctac cggcccgacg ggcgcacggc
     5101 acgtctcacg ctccaggaca cgcggctgcc ggcgccgtgc ggcgccgcgg ccagctccct
     5161 tgctgcgctc acgctgcacg acctcgagca cggtcctccc gccggccagg tcgagatgct
     5221 cgtgctgccg ctcgatcccg actacatcgc gtgtgcgtcc gcgagcgagt cggtccggtc
     5281 acgacacgtc ccgtcgcaag cgggttcgga cgcctccagc tccagcggga aggtcaaggc
     5341 gccgaagaag accaggcatg tcaacccggt ctacccggcg gccatgcagg agctgaaggt
     5401 gcaggggacc gtgtgggtcg aggccacgat cacgccggcg ggatgcgtgg ctgccggccg
     5461 gctcgtaaag agcccgcacc tgctgcttgg cctgtcctcg ctgtgggccg tctccggctg
     5521 gcggttcgag ccgacgctgc tcgccggcaa accggtccca gtcatcattt ccgtggcggt
     5581 ggactacaag ctgcgctgac ccgtgacgcc tgggcaaagg gaaatgacgc ggcgactcaa
     5641 ggccggaacc aagcgggtga ccccggtggc cagagcgggc ggccgttggc tgcgcgacaa
     5701 gcggcgcgtc ttgacgccga atcgaaggcg gcattgcctg acttagaccg gctgcgtgac
     5761 gttcaatccg gccttccgcc tggctcgatg cggccgccag cctcgttctg tcgtcgtcgg
     5821 cgctcacttc gaaccttccc gcttctcccg ctccgcttcc cgctgcctgg cctcgagctc
     5881 ggccttgtag cgccgacccc aggagtagcg gcccgtgggt tcgggcgtcg ggggagcggc
     5941 gttcttccgc ctctcctcga gttggcgacg ccactcctcc gcgaggcgct gctgggtgtg
     6001 ccgccactgc tcgcgcgtca tcgagcggac cagtctcagg cagtcgcggc tgtcagtcga
     6061 ggcaccagcg cgaggctcgg cgccgaagcg ctcgaccatc ctttgataca ggcgcatctc
     6121 cgggtccctc tcccagtagg cctccaggag ggcgcgcatc atgatgtcgc cgtggaacag
     6181 gattcgagcg gccatcgcac gaattcactc gccgggtaac tggctccacc cagcatcggc
     6241 cgggcgtgtg acaggacagg tcacaccgcc agatcttgca cctgtcgtac cgcggaacca
     6301 ccttggcacg taggcctgca ccatcggcgg gaggaggccg tgtcgcacgg cgccacaggt
     6361 gcctagcgcg ggcaggattc tcgccgggat gtcctcatcc tgcccttcga gaggctcgcc
     6421 a
//
LOCUS       DAOPQX010000203         6415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13238_length_6415_cov_3.766352, whole genome shotgun sequence.
ACCESSION   DAOPQX010000203 DAOPQX010000000
VERSION     DAOPQX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13238_length_6415_cov_3.766352"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1154)
                     /gene="moaA"
                     /locus_tag="PKK95_11145"
     CDS             complement(138..1154)
                     /gene="moaA"
                     /locus_tag="PKK95_11145"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOC18819.1"
                     /translation="MPLVDLLGRPLRGLRVSVTDRCNLRCQYCMPEEEYTWLPREDLL
                     TFDEIVVLVRQFVRLGVNKIRLTGGEPLLRRGLPSLVAALASLPGLDDLALTTNGVLL
                     GGQAQALRDAGLRRITVSLDTLRPDRFTALTRLHTHDRVLEAIEVAARVFGSLKIDTV
                     VMRGINDDEMVSLVEFGRRLNAEVRFIEYMDVAGATRWLPEQVVSRVEMLERLSAHYG
                     PLEPIEEGTWAPADRFRLRDGATVGVIASTTTPFCGTCDRARLTADGLWFRCLYATEG
                     MDLRGPLRAGVPLEELGARIERAWRARADQGAELRLAERERVALPAVSLKGRPHLEMH
                     TKGG"
     gene            complement(1190..2245)
                     /gene="amrS"
                     /locus_tag="PKK95_11150"
     CDS             complement(1190..2245)
                     /gene="amrS"
                     /locus_tag="PKK95_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOC18820.1"
                     /translation="MASTTLGQELERQTREGDLYERYNGDWTRCFACGHRCPIPNGAA
                     GVCKVRFNRGGRLMVPWGYVAGAACDPIEKKPFFHAFPGALAYSFGMLGCDLHCSYCQ
                     NWVTSQALRDPAAMLISRDSSPAELVAEAVRRGARAVVSTYNEPLITAEWAVEIFKLA
                     RKAGLATGFVSNGNGTPEVIEYLRPWVDLYKIDLKGFDDKRYRQLGGRIEPILDTIRR
                     VHAAGLWTEIVTLLVPGFNDDDDELRQLTAFVAGVSPDIPWHVTAYHEDYRMSGTGNT
                     TVGMLERAARVGREAGLRYVYAGNVPGAVGDLENTRCPSCRRLLVERTGYRVRRFEVN
                     GQGACPGCGTVIPGRWH"
     gene            2312..3463
                     /locus_tag="PKK95_11155"
     CDS             2312..3463
                     /locus_tag="PKK95_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOC18821.1"
                     /translation="MSTAPATAAARVPATRLVSLDVFRGATMAAMVLVNNPGDWGQVY
                     APLLHAEWHGWTPTDLIFPFFLFIVGVAMTLSRATLGPWGRILRRGATIVGLGALLAG
                     FPYYPLATWRIPGVLVRIGIGYLAATALFRWTLPSGARDDRKHAVRLAWVAGLLVIGY
                     WAAMVLAPYPGHLPGDLSKEGNLAAFIDRAVIGQAHMYRQRGWDPEGLASTLPAIGTI
                     LAGVITGFWLRAGVEARAKAAVLAAAGLALLALGLLWDLAFPINKNLWTSSFVAFTGG
                     AAAFSLAACYYLVDVLGWRWWTRPFVILGVNAITLYVLAGLSARLLGLIMVTGPEGTP
                     ISLQRFVYTSWFAPLADPKNASLLYACAYLAVLFVVLYGMYRKGIYIRA"
     gene            complement(3493..4230)
                     /locus_tag="PKK95_11160"
     CDS             complement(3493..4230)
                     /locus_tag="PKK95_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18822.1"
                     /translation="MLSPLLVSAGLTAVLAIPPVAAPQAVDVREPYPALTAAGRDWLR
                     DREPILFAGERYFPAGPAVHFEPDLMVPTGSYDGVPLYADTSLEPYSRVLVPIGRGLL
                     QPYERLREGGLAGTTGSRTPSFPVPLRPDVRIQSNRWQDDSPGQRPPQTPRASIPAPS
                     PPEERQPGIVSAREPQGNRGIWIQYDGRRWESAGDAVAHDAEFKKVGEYRGFPVYARD
                     SAPAGGAAQIYIPSRDDMLAPYRAATD"
     gene            4414..4566
                     /locus_tag="PKK95_11165"
     CDS             4414..4566
                     /locus_tag="PKK95_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18823.1"
                     /translation="MNYLAFALIVLAAAGAAALARRHHRRAGRSIDAGVVSQQWLAES
                     RREEEA"
     gene            4563..5282
                     /locus_tag="PKK95_11170"
     CDS             4563..5282
                     /locus_tag="PKK95_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC18824.1"
                     /translation="MIDRSSFGPELRRAREARGLTLEEIAEATKVNVALYRGLENNDL
                     SRWPSGLFRRAFVRSYAETVGLDPEDTCRRFVRFFPEEGDVVPEMHAVPPPSASIAPP
                     ASSEDPPRLVLAAAPPQGPSGPPAWRRVTAPLVDVGLAGLLGWGAGTLSTLLAGPDWF
                     WTAAGATVATAYVAVVVMLGTTFGARWLLPGQERPSPVTETPQLSRRTGGERPVLVAS
                     RHHAPRAAASGRAAARRQGRR"
     gene            5403..6164
                     /gene="surE"
                     /locus_tag="PKK95_11175"
     CDS             5403..6164
                     /gene="surE"
                     /locus_tag="PKK95_11175"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOC18825.1"
                     /translation="MARILITNDDGVRSPGIGALAEALDGLGEVYVLAPTREASAIGH
                     ALTLHHPVRLEHLKDRVYAVDGTPTDCVNIAVAAVLHGLPDLVVSGINKGLNVGDDVT
                     YSGTVSGALEASLLGVRAIAVSLQEAAGEWHWDHAARVARRVAEAVLRDGLPPRTFLN
                     VNVPRGEPKGVRVTVQGKRNHATTVSERHDPRGRPYYWIGEGQDEWLPNGESDYEAIR
                     AGFVSVTPLQPDLTAHASLEAIRQLAGSAQLTADS"
     gene            6339..6415
                     /locus_tag="PKK95_11180"
     tRNA            6339..6415
                     /locus_tag="PKK95_11180"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6373..6375,aa:Pro,seq:cgg)
BASE COUNT          907 a         2232 c         2314 g          962 t
ORIGIN      
        1 ccgccctggc gctcgagaac gccgggcgcg gcggcgagct gtcgtccgcc cccgcgctgc
       61 tcgacacgct gcgaaccgag ctggccgccg tgcgccaggc gctgagcaat ctcgcggcgg
      121 gccgctgaga aggattgcta cccgcccttc gtgtgcatct cgaggtgcgg gcgccccttc
      181 agcgacactg cgggcagcgc gacccgttcg cgctcggcca gccgcaactc ggcgccctgg
      241 tcggcgcggg ctctccaggc gcgctcgatt cgggcgccca gttcctccag cggcaccccg
      301 gcccgcagcg gcccccgcag gtccattccc tccgtggcat agagacagcg gaaccacagg
      361 ccgtcggcgg tgaggcgcgc ccggtcgcag gtgccgcaga agggagtcgt cgtggacgcg
      421 atgacgccca ccgtggcgcc gtcgcgcagg cggaaccgat ccgcaggggc ccacgtgccc
      481 tcctcgatcg gctcgagcgg cccgtagtgc gccgacaggc gctcgagcat ctcgacgcgt
      541 gacaccacct gctcgggcag ccaccgggtg gcgccagcga cgtccatgta ctcgatgaag
      601 cgcacctcgg cgttcaatcg gcggccgaac tccacgagcg acaccatctc gtcgtcgttg
      661 atgccgcgca tcaccacggt gtcgatcttc aggctgccga agacgcgggc cgcaacctcg
      721 atcgcctcga gtacccggtc gtgagtgtgg agacgcgtga gcgccgtgaa ccggtcgggc
      781 cgcagggtgt cgaggctgac ggtgatgcgg cgaaggccgg cgtcgcgcag cgcctgcgcc
      841 tgcccgccga gcagcacgcc gttggtggtg agagcgaggt cgtcgagccc gggcaggcta
      901 gccagcgccg cgacgagcga ggggagcccg cgccgcagca gcggctcgcc gccggtgaga
      961 cggatcttgt tcacgccgag ccggacgaac tgacgcacga ggacgacgat ctcgtcgaag
     1021 gtgagcaggt cttcgcgggg cagccaggtg tactcctctt cgggcatgca gtactggcag
     1081 cgcaggttgc agcggtcggt gaccgacacc cgcagtcccc gcaacggccg tccgaggagg
     1141 tccaccaggg gcataccaga gatcttaccg gagaaaccgg cggcccccgt caatgccagc
     1201 ggccggggat gacggtgccg cagccgggac acgcgccctg tccgttcacc tcgaaccgtc
     1261 gaacgcggta ccccgtgcgc tcgaccagga ggcggcggca ggaggggcag cgcgtgttct
     1321 cgaggtcgcc gaccgccccg ggcacgttgc cggcgtacac gtagcgcaat cccgcctcgc
     1381 ggccaacccg ggcggcccgt tcgagcatgc cgaccgtcgt gttgccggtc cccgacatcc
     1441 ggtagtcctc gtggtaggcg gtcacgtgcc acgggatgtc aggcgacacg cctgcgacga
     1501 acgcggtcag ctgccgcaac tcgtcgtcgt catcgttgaa gccgggcacg agcagggtca
     1561 cgatctcggt ccacagcccg gcggcgtgaa cgcgccggat cgtgtcgagg atcggctcga
     1621 tgcggccgcc cagctggcgg tagcgcttgt cgtcgaaacc cttgaggtcg atcttgtaga
     1681 gatccaccca cgggcgcagg tactcgatca cctcgggcgt gccgttcccg ttggagacga
     1741 agccggtggc caggcccgcc ttgcgcgcca gtttgaagat ctcgacggcc cactcggccg
     1801 tgatcagcgg ttcgttgtag gtgctgacca ccgcccgggc gccgcggcga accgcctcgg
     1861 ccaccagctc ggcgggcgac gagtcgcgcg agatgagcat cgccgccggg tcgcgcagcg
     1921 cctgcgaggt gacccagttc tggcagtaac tgcagtgcag gtcgcagccg agcatgccga
     1981 agctgtaggc gagcgccccc gggaaggcgt ggaagaacgg cttcttctcg atcggatcgc
     2041 acgccgcgcc ggcgacgtac ccccacggaa ccatcagccg gccgccgcgg ttgaagcgga
     2101 ccttgcacac gccggccgcg ccgttcggga tggggcagcg gtgcccgcac gcgaagcagc
     2161 gggtccagtc gccgttgtat cgctcgtaca ggtcgccctc gcgggtctgc cgctcgagct
     2221 cctggccgag ggtggtgctt gccatgttcc agatctacca ccacaccgcc ccggatgcga
     2281 cctgaacggc tttgagctat cctgtcgcgg catgagcacc gcgccagcca cagccgccgc
     2341 ccgcgtcccc gccacccgcc tggtttccct cgacgtcttc cggggcgcga cgatggccgc
     2401 gatggtcctg gtgaacaacc cgggcgactg gggccaggtg tacgcccccc tgctgcacgc
     2461 cgaatggcac ggctggacgc cgaccgacct catcttcccc ttctttctct tcatcgtcgg
     2521 ggtggcgatg acgctgtcgc gcgccacgct cgggccctgg ggacgcatcc ttcgccgcgg
     2581 ggcgaccatc gtcggcctgg gagcgctgct cgcggggttc ccgtactatc cgctggcgac
     2641 gtggcgcatc cctggcgtgc tggtcagaat cgggatcggc tacctggcgg cgaccgcgct
     2701 gttccggtgg acgctgccct cgggcgcgcg cgacgatcgc aagcacgccg tgcggctggc
     2761 gtgggtggcg ggcctgctcg ttatcggcta ctgggcggcg atggtgctgg cgccgtaccc
     2821 gggccacctg ccgggcgatc tttcgaagga aggaaacctg gccgccttca tcgaccgcgc
     2881 ggtgatcggc caggctcaca tgtaccggca gcgcggatgg gacccggagg ggcttgcgag
     2941 cacgctgccg gcgattggca ccatcctcgc cggggtgatc accggcttct ggctgcgcgc
     3001 cggggtcgag gcgcgcgcga aggcggcggt gctggccgcg gccggcctcg cgctgcttgc
     3061 cctggggctg ctgtgggacc tggccttccc catcaacaaa aacctttgga ccagctcgtt
     3121 cgtggccttc acgggcgggg cggccgcgtt ctcgctcgcc gcctgctact acctggtgga
     3181 cgtgctgggc tggcggtggt ggaccaggcc cttcgtgatt ctcggggtca acgcgatcac
     3241 gttgtacgtg ctcgccgggc tcagcgccag gctgctcgga ctcatcatgg tcaccgggcc
     3301 agagggcacc ccgatctcgc tgcagcggtt cgtctacacg tcgtggttcg cgccgctcgc
     3361 cgacccgaag aacgcgtcgc tgctgtacgc ctgcgcgtac ctcgccgtgc tgttcgtcgt
     3421 cctttacggg atgtaccgga aggggatcta catcagggcg tagatcccct ccggcgttcc
     3481 gctgacggct cgctagtcgg tggctgccct gtagggcgcg agcatgtcgt cacggctcgg
     3541 aatgtagatc tgcgccgccc cgccggcggg ggccgagtcg cgcgcgtaga cggggaagcc
     3601 gcggtactca cccaccttct tgaattcggc atcgtgagcc accgcgtcgc cggccgactc
     3661 ccagcgacgg ccgtcgtact gaatccagat gccgcggtta ccctggggct cgcgcgccga
     3721 gacgatcccc ggctgccgct cctcgggagg ggaaggggct ggaatggacg cgcgcggcgt
     3781 ctgcggcggc ctctgacccg gcgagtcgtc ctgccaccgg ttgctttgga tgcgaacgtc
     3841 gggccgcaac gggaccggga acgacggggt gcggctcccg gtggtgccgg cgaggccacc
     3901 ctcgcgcagg cgctcgtagg gctgcagcag ccctcgtcct atcgggacca gtacccggct
     3961 gtacggctcg agcgatgtgt cggcgtacaa ggggacgccg tcgtacgagc ccgtcggcac
     4021 catcaggtca ggctcgaaat ggacggcggg accggcgggg aagtagcgct cgccggcgaa
     4081 gaggatgggc tcgcggtcgc gcagccagtc acggccggcg gcggtgagcg cgggatacgg
     4141 ctcccgaacg tccacggcct gcggggccgc gaccggcggg atcgccagca cggccgtcag
     4201 gccggcggag acgagcaggg ggctgagcat gggaacctcc gcggagcgag gtgccaagcg
     4261 tgtgccagcc aaccgcgcga ctggccgggc gcctccgctt ggtggcaatt tgccgttcct
     4321 gacggatcag cttgcaagca cgggcatact cgtcccatac ttcgttgccg cgttcacggg
     4381 gcccctgtcc ctcctcttcg cggagtccgt tccttgaact acctcgcgtt cgccctcatc
     4441 gtgttggcgg ccgccggcgc ggccgctctc gcgcggcggc atcaccgccg ggcggggcgc
     4501 tcgatcgacg cgggagtcgt cagccagcag tggctggctg agagccggcg ggaggaagag
     4561 gcatgatcga ccgctcctcg ttcggccccg aactgcggcg tgcccgggag gcgcgcggcc
     4621 tcaccctgga agagattgcc gaggcgacga aggtcaacgt ggcgctctac cgggggctgg
     4681 agaacaacga cctctcgcgc tggccgtcag ggctgttccg gcgggcgttc gtgcgcagct
     4741 acgccgagac ggtcgggctg gatcccgagg acacctgccg ccggttcgtg cgcttcttcc
     4801 ccgaggaagg cgacgtcgtg cccgaaatgc acgccgtgcc gcctccgtcg gcctcgatcg
     4861 ccccgcccgc ttcctccgag gatccgcctc ggctcgtcct ggcggctgca cccccccagg
     4921 gcccctccgg cccgccggcc tggaggcgcg tcacggcccc gctggttgac gtcggcctgg
     4981 ccggcctcct cggctggggg gcgggcacgc tgtcaacgct cctggcgggg cccgactggt
     5041 tctggacggc cgctggggca acggtcgcga cagcgtacgt ggcggtggtc gtgatgctcg
     5101 gcaccacgtt cggagcccga tggctcctgc cgggccagga acggcccagt cccgtgaccg
     5161 aaacaccaca gcttagccga aggacgggcg gcgagaggcc agtgctggtg gcgagccggc
     5221 accacgcgcc gcgcgcggcc gcgtctggcc gcgcggcggc aaggcgtcag ggccgccgct
     5281 agcctggagt gcgggaccgg cgcgccactc tcgatgggcg ccgcgcccgc cgtgctggcg
     5341 accgtgctct cttcgccctg gtccgcgagc ctgcgaggac cgtgggatac tgatcgttcc
     5401 ctatggctcg catcctcatc accaacgacg atggcgtgcg gtcgccgggc atcggggcgc
     5461 tggccgaggc gctcgacggc ctcggcgagg tctacgtgct cgcaccgacc cgcgaggcca
     5521 gcgcgatcgg ccacgccctg acgctccacc acccggtgcg gctcgagcac ctgaaggacc
     5581 gggtctacgc cgtggacggg acgcccaccg actgcgtgaa catcgccgtc gcggcggtgc
     5641 tgcacggcct gcccgatctc gtggtgtcgg gcatcaacaa ggggctgaac gttggcgacg
     5701 acgtgacgta ctcgggaacc gtgtcaggcg ccctcgaggc ctcgctgctg ggcgtgcggg
     5761 caatcgcggt gtcgctgcag gaggcggcgg gcgagtggca ctgggaccac gcggcgcgcg
     5821 tggcgcgccg ggtggcggag gccgtgctgc gcgacggcct gccgccgcgc acgttcctga
     5881 acgtcaacgt gccgcggggc gagccgaaag gcgttcgcgt caccgtgcag gggaagcgga
     5941 accacgcgac gacggtcagc gagcggcacg atccgcgggg ccggccgtac tactggatcg
     6001 gggaagggca ggacgagtgg cttccgaacg gcgagtcgga ctacgaggcg atccgcgctg
     6061 gcttcgtctc ggtgacgccg ctgcaacccg acctgacggc gcacgcgtcg cttgaagcga
     6121 tcaggcagct ggcaggcagc gcacagctga cagcggacag ctgagcggat cacgggtgtc
     6181 acgctgcatt cgtggtccca cgttcaacgc ggagacgcgg aggcgcggag acgacagaac
     6241 ggcgggcggc aaacaccgga catttcacgt actaccaata ccggatgcgg atgccgcttg
     6301 tgtcgcccgg tatgccgagg tagaatgacg agtccggtcg gggcgtggct cagcctggta
     6361 gagcactcgg ttcgggaccg aggggtcgga ggttcaaatc ctctcgcccc gacca
//
LOCUS       DAOPQX010000204         6379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13346_length_6379_cov_4.086022, whole genome shotgun sequence.
ACCESSION   DAOPQX010000204 DAOPQX010000000
VERSION     DAOPQX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13346_length_6379_cov_4.086022"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKK95_11185"
     CDS             <1..430
                     /locus_tag="PKK95_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOC18826.1"
                     /translation="MRTMLQAIGATVAQPRFRTMLVALFALVALLLAAIGVYGVMAYG
                     VTQRAHELGVRMALGAADRHVVRLVLVESLALTALGVCLGLAGGAAATRLLAGLLFET
                     RPLDPPTFLAVPLVLAAIALAASYVPARRALRVEPAVVLR"
     gene            complement(699..2384)
                     /locus_tag="PKK95_11190"
     CDS             complement(699..2384)
                     /locus_tag="PKK95_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18827.1"
                     /translation="MGPHTISAYGLAIALAAFPAGSFAQSHQHYTHAEEGNKPAPSGA
                     LAPRLLNLGDHTFTVTTRSPEAARFMSQGLNLAYGFNHAEAARSFREAARLDPDCAMA
                     YWGQALVIGPNINAPMDPADEPRALALVKEAMARKGNASPREQAYIDALAERYSGRPE
                     DRKERDQAYAAAMKRVAERFPDDLDAAALYAEAVMDLRPWGYWMRDGQPYEGTLEIQR
                     LLESVMQRNPRHPGALHFYIHLMEPTSEATKAEAAADTLLTLAPAAGHLVHMPSHIYQ
                     RVGRYADAVRSNELAVLADEDYITQCRAQGLYPMAYYPHNIHFLWFAAQMDGQSARAI
                     EAARNVASKIDDKALAEMPMLAGFRIVPYFALTRFHRWDEMLREKEPPAGQTFMRGIW
                     HYARGLAFLGRGEVAEAERELESVRQALASKDEVLDQPLFSPNTARAILEIAPEVLAG
                     EIAAARKQYDIAIAHLDRAVRLEDGLVYTEPAEWHYPPRHALGAVLLAAGRPAEAETV
                     YWEDLRRNPENGYALTGLLQALRAQSKDEDAALVEKRLAAAWARADVRLASSR"
     gene            complement(2431..2865)
                     /locus_tag="PKK95_11195"
     CDS             complement(2431..2865)
                     /locus_tag="PKK95_11195"
                     /inference="COORDINATES: protein motif:HMM:NF016211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOC18828.1"
                     /translation="MEPPAGMSRVRRVLFTATGFAATGLGVLGIILPLMPATPFLLLA
                     AYFFSRSSPRFHRWLLTNRVFGSYIENYRSGRGLSLTEKVVTIATLWIALAITAATAV
                     GVWWGRALLLAVAIGVTTHLLRLPTCRPAPVDERLPTGQVRR"
     gene            complement(2871..5156)
                     /locus_tag="PKK95_11200"
     CDS             complement(2871..5156)
                     /locus_tag="PKK95_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC18829.1"
                     /translation="MRQLGTLLLSFFVGIFLPPLAAADAVSVKGRVLRADGAPIAGAS
                     VNVAGAAVAPETTTGDDGGFALELPAGEHVLRVRAEGFRPLVVRVRVGDEPAAPHDLR
                     LEPAVLRHEEHLVVTAARVEQPAAGSPRAIAVLDREQLDERMPRSTPEALADLPGVLL
                     QKTNHGSGSPYVRGLLGNQVLVLVDGVRLNNSTFRYGPNQYLATIDPSSLEKVEVMRG
                     SGSVLYGSDAIGGVINLVTRRARLSSGPMRVSGGVSGKWMTGGMEQGGRADAEVSSSS
                     AAVRGGVSVRRFGDLVAGGSLGVESPSGYDEVSGDASGVLRLGRTSLLSVSYQHLHQG
                     DVPRWDQVAQRGFARYAFDPQVRQLASGAWEWFPGSGPFARVDAGLSFHRSRERRERQ
                     TRGSSVLIVEQDTVRTLGATLTAETRPVHGWTVLAGADIYNDWIGSWRRDRNTTGGSE
                     VLKRGLYPDGSRASSAAAFVTGSWNRGALHLDVGGRHSRYNVSADDSAFGSLDLSPSA
                     TVGSVAASYRLAPGLQLVGSVSQSFRAPNVDDVSTLGLFDNGIEVPSPNLSPERGIGY
                     EAGFRARAGRVAFSAAAFRTSLRDLIDRVPSSYAGSEYLEGQRVYQKANVARAFVRGV
                     EAEAEASLTGSLHAAAHVSYTYGHQPSTGQPMRRIPPLNGLLSLRWSGPDRLFVEAQW
                     RVAAAQRRLAPGDISDHRIGPDGTGGWHVLDVYLGRPLGGGVSLNAGLVNLFDEAYRV
                     HGSGIDGMGRALWVGFGFVRQ"
     gene            complement(5604..>6379)
                     /locus_tag="PKK95_11205"
     CDS             complement(5604..>6379)
                     /locus_tag="PKK95_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18830.1"
                     /translation="YVERRRALFAICREARLRNEPPPIIDYTGEEQRIWREVTPRLDE
                     LHNRHASSIYLKAKRDLRISEDAIPQLRTLSEQLKGVSGMHLVPAEGPIPYRTFYTYI
                     AERGFPVTQFIRHGSKPEFTPEPDMIHDCLGHVPPLVNHDYAALLTQIGRAAVAVPTG
                     DKVLALKRLSWFSIEFGLIEEAGETKVFGAGILSSMGEIPNSLSSNVERRPFVTGEVI
                     ETDYDPSRMQDLLFVIPSFAFLRNEVEQLVQRFGVPITT"
BASE COUNT          906 a         2268 c         2272 g          933 t
ORIGIN      
        1 gatgcggacg atgttgcagg ccattggggc gacggtggcg cagccgcgat tccgcaccat
       61 gctggtggcg ctcttcgcgc tcgtggcgct gctgctggcc gcgatcgggg tgtacggcgt
      121 gatggcttac ggcgtcacgc agcgcgccca cgagctgggc gtgcgcatgg cgctcggcgc
      181 ggccgaccgc catgtcgtgc gcctggtgct cgtcgagtcg ctcgccctga cggcactggg
      241 cgtctgcctg gggctggccg gcggcgcggc ggccactcgc ctgctcgcgg ggctgctgtt
      301 cgagacgcgg ccgctggatc cgccgacctt cctcgcggtg ccgctcgtgc tggcggcgat
      361 cgccctcgcg gcgagctacg tccccgcgcg ccgcgcgctc cgcgtggagc ccgcggtcgt
      421 actgcggtag cgaaggttcc gggggggccg gcggcggtgc atcccctgcc cgccatggcc
      481 agtggcccgc aaccatgatc gggatccggg tcctgtcggg gcagcgacac cgttttgtcc
      541 gccacccgct ccggttgcgg gctgacggca ctcgcgccgc cgcactcccc gagtcgctac
      601 gcttcgccgt cagagacgtg cgacatccga cttcgtggac ttgacttcgc cacctcgcga
      661 cttcgtggac ctcgcgactt cgtggactgg cgtccctgct accgtgacga ggccagcctg
      721 acgtcggcgc gggcccaggc cgccgcgaga cgcttctcca ccagggcggc gtcctcgtcc
      781 ttcgactgcg cccgcagcgc ctgcagcagt cccgtcagcg cgtagccgtt ctccgggttg
      841 cgccgcaggt cctcccagta gaccgtctcg gcttcggccg gacggccggc cgcgagcagc
      901 accgcgccca gcgcgtggcg cggcgggtag tgccactcgg caggctcggt gtagacgagg
      961 ccgtcctcga ggcgaaccgc ccggtcgagg tgggcgatgg caatgtcgta ctgcttgcgg
     1021 gccgccgcga tctcgcccgc gagcacctcc ggcgcgatct ccaggatggc gcgcgcggtg
     1081 ttcggcgaga acagcggctg atcgagcacc tcatccttcg aggcgagcgc ctgccgcacc
     1141 gattccagct ctcgctcggc ttcagcaacc tcgcccctgc cgaggaacgc cagcccgcgc
     1201 gcgtagtgcc agatcccgcg catgaaggtc tgcccggccg gcggctcctt ctcgcgaagc
     1261 atctcgtccc accggtggaa gcgcgtgagc gcgaagtacg ggacgatgcg gaagcccgcc
     1321 agcatcggca tctcggcgag cgccttgtcg tcgatcttcg acgccacgtt ccgcgccgcc
     1381 tcgatcgccc gcgcgctctg cccgtccatc tgcgccgcga accagaggaa gtggatgttg
     1441 tgcggatagt acgccatcgg gtagaggccc tgcgcgcggc actgggtaat gtagtcctcg
     1501 tctgccaaca ccgccagctc gttgctgcgc accgcgtcgg cgtagcggcc gacgcgctgg
     1561 tagatgtgcg acggcatgtg gacgaggtgg ccggcggccg gggcgagcgt gagcagcgtg
     1621 tcggcagcgg cctcggcctt cgtggcttcc gacgtcggct ccatcaggtg gatgtagaag
     1681 tggagcgccc ccgggtgccg cgggttccgc tgcatcaccg attcgagcag ccgctggatc
     1741 tcgagcgtgc cctcgtaggg ctggccgtcc cgcatccagt aaccccaggg cctgaggtcc
     1801 atcacggcct cggcgtacag cgcggccgca tcgaggtcgt cggggaatcg ctcggccacc
     1861 cgcttcatcg ccgcggcata ggcctggtcg cgctccttgc ggtcttcggg ccgcccggag
     1921 tagcgctccg cgagcgcgtc gatgtatgcc tgctcgcgcg gcgacgcgtt ccctttacgg
     1981 gccattgcct ccttcacgag ggcgagggcc cgcggctcgt cggccggatc catgggcgcg
     2041 ttgatgttgg gtccgatcac gagggcctgg ccccagtagg ccatcgcgca gtcgggatcg
     2101 agccgcgccg cttcgcggaa cgatcgtgcc gcctcggcgt ggttgaagcc gtaggcgagg
     2161 ttgagcccct ggctcatgaa gcgcgccgcc tcgggcgagc gcgtcgtgac cgtgaaggta
     2221 tggtcgccga ggttcaacag gcgcggcgcc agcgcgccgc tcggcgccgg cttgttgcct
     2281 tcctcggcgt gggtgtagtg ctggtgcgat tgtgcgaagc ttccggccgg aaacgctgcg
     2341 agcgcaatgg caaggccgta ggcggagatg gtgtgggggc ccatcgtctc ctcctctgct
     2401 gactgatgat agcgcgagcc ggacgagccc tcagcggcgg acctgcccgg tcggcagccg
     2461 ctcgtccacc ggggccgggc ggcacgtcgg cagcctcagc aggtgcgtgg tgacgccgat
     2521 ggccaccgcg agcagcagcg cgcggcccca ccacacgccc acggccgtgg cggcggtgat
     2581 cgcgagcgcg atccagagcg tggcgatcgt cacgaccttc tcggtgagcg agaggccgcg
     2641 gccgctccgg tagttctcga tgtacgaccc gaagacgcgg ttggtgagca gccagcggtg
     2701 gaagcgcggc gagctgcgcg agaagaagta tgcggccagc agcaggaacg gggtggccgg
     2761 catcagcggc aggatgatgc cgagcacgcc gaggccggtg gcggcgaacc cggttgcggt
     2821 gaagagaacg cgccgcacgc gcgacatccc ggctggtggt tccactgcgc tcattgtcgc
     2881 acgaacccga accccaccca cagagcccgg cccatcccgt cgatgcccga gccgtgcacg
     2941 cggtaggcct cgtcgaagag gttcaccagg cccgcgttca gcgagacgcc cccgccgagc
     3001 ggccgcccca ggtagacgtc gagcacgtgc catccgccgg tcccgtcggg gccgatgcgg
     3061 tgatcggaga tgtcgcccgg cgccaggcgc cgctgcgcgg ccgccacccg ccactgcgct
     3121 tccacgaaca gccggtcggg gcccgaccac cgcagcgaca gcaggccgtt gagcgggggg
     3181 atgcggcgca tcggctgccc ggtcgacggc tggtgcccgt aggtgtagct cacgtgcgcg
     3241 gcggcgtgca gcgagccggt caggctggcc tcggcctcgg cctcgactcc acggacgaac
     3301 gcgcgcgcca cgttggcctt ctggtagacg cgttgccctt ccaggtactc gctgccggcg
     3361 taggacgatg gcacccggtc gatcaggtcg cggaggctcg tacgaaatgc cgcggcggag
     3421 aaggcgactc gcccggcgcg ggcccggaaa cccgcttcgt acccgatccc gcgctcgggc
     3481 gagaggttcg gcgacgggac ctcgatcccg ttgtcgaaga gccccagcgt gctgacatcg
     3541 tccacgttcg gcgcccggaa cgactgcgag accgacccga ccagctggag gccgggggcg
     3601 aggcggtagc tcgccgccac cgatcccacc gtggccgacg gcgacaggtc gagcgacccg
     3661 aacgccgagt cgtctgcaga cacgttgtag cggctgtggc gcccgccgac gtccaggtgc
     3721 agtgccccgc ggttccacga accggtgacg aacgccgccg ccgacgaggc ccgcgagccg
     3781 tcgggataaa gcccgcgctt caggacctcc gagccgcccg tcgtgttgcg gtcgcgccgc
     3841 cagctgccga tccagtcgtt gtagatgtcg gcccctgcca gcaccgtcca cccgtgaacg
     3901 ggacgcgtct cggcggtgag ggtcgccccc agcgtccgca ccgtgtcctg ttcgacgatc
     3961 aggacggagg agccgcgggt ctggcgttcg cggcgctcgc gcgagcgatg aaacgagagc
     4021 cctgcgtcta cccgcgcgaa cggccccgac ccggggaacc actcccaggc gccgcttgcc
     4081 agctggcgca cctgcgggtc gaacgcgtag cgcgcgaagc cgcgctgcgc gacctggtcc
     4141 cagcgcggca cgtcgccctg gtgcaggtgc tggtaggaca ccgacagcag cgacgtacgg
     4201 cccagcctga gaacgcccga cgcatccccc gaaacctcgt cgtagcccga gggcgactcg
     4261 accccgagcg agccgccggc gacgaggtcg ccgaagcgcc gcaccgacac tccgccgcgc
     4321 accgcggccg aagacgacga gacctccgcg tcggcgcggc cgccctgctc catcccgccc
     4381 gtcatccatt tgcccgagac gccgcccgac acccgcatcg ggccgctgct cagccgcgcc
     4441 ctccgggtca ccaggttgat gacgccaccg atcgcgtcgc tgccgtacag caccgacccc
     4501 gatccgcgca tcacctccac cttttccagg gacgaggggt cgatggtcgc gaggtattga
     4561 ttcggcccgt agcggaacgt gctgttgttg aggcggacgc cgtccacgag caccagcacc
     4621 tggttgccga gcaggccgcg aacgtaggga gagcccgagc cgtggttcgt cttctgcagc
     4681 aggacgcccg gcaggtcggc gagcgcctcg ggcgtggacc gcggcatccg ctcgtcgagc
     4741 tgctcgcggt cgagcacggc gatcgcccgc ggcgagcccg cggccggctg ctccactcgc
     4801 gccgctgtca cgacgaggtg ttcctcgtgg cggagcacgg ccggctcgag gcgcaggtcg
     4861 tgcggcgcgg ccggctcgtc gccgacgcga acgcggacga ccagcggccg aaagccttcc
     4921 gcccgcacgc ggaggacgtg ctcgccggca ggcagttcga gcgcgaaccc gccgtcgtcg
     4981 ccggtcgtgg tctccggggc gacggccgcg cccgccacgt tgacgctggc gccggcgatg
     5041 ggcgcgccat cggcccgcag cacgcggcct ttcaccgaga cggcatcggc cgcggccagc
     5101 ggcggcagga agattccgac aaagaaggac aggagaaggg ttccgagctg tctcacgttt
     5161 cgcatcctgt ggggctgacg accagaacga gagaaggtac cacagaccgg cgcccggccg
     5221 cgaccgggac aggagacagc gcggacagcg ggcaccggct cttgagccag atcactgacg
     5281 ccgcccttgg agccgcgggc cgttcgcgga gaaaaccgtg tggagcaggc cgatcaggtg
     5341 cggacaaaac gcaccgcagg cctgcggaca ggcctgggga taggggtccc caggcgcgtt
     5401 cggacggacg gtaagccgag gtgcgttttg ccggcctgcg gaacccggtt ttcggagcga
     5461 atggcccgcg gctccagaag cgcgcagaac cgctcggcgg agcccggctc ggcccaacca
     5521 aaaggtgtct ggctgccccg ccacccgctc cggttgcggc aaatggcact cgcggagtcc
     5581 ccgcgagccc gtcagctgcc gccttacgtc gtgatcggga ccccgaagcg ctgcacgagc
     5641 tgctcgacct cgttccgcag gaacgcgaac gacgggatca cgaacagcag gtcctgcatc
     5701 cgcgaggggt cgtagtcggt ctcgatcacc tcgccggtga cgaaggggcg ccgctcgacg
     5761 ttcgacgaca gcgagttcgg gatctcgccc atcgaggaga ggatgcctgc cccgaacacc
     5821 ttcgtctcgc ccgcctcctc gatcagcccg aactcgatcg agaaccagct caggcgcttg
     5881 agcgccagca ccttgtcgcc ggtggggacc gccaccgccg cccggccgat ctgcgtgagc
     5941 agcgccgcgt agtcgtggtt cacgagcggc ggcacgtggc ccaggcagtc gtggatcatg
     6001 tcgggctcgg gcgtgaattc cggcttcgac ccgtgccgga tgaactgcgt caccgggaac
     6061 ccgcgctccg cgatgtaggt gtagaaggtc ctgtagggga tcggcccctc ggcgggcacc
     6121 aggtgcatgc cgctcactcc cttcagctgt tcgctgagcg tgcgcagctg cgggatcgcg
     6181 tcctcgctga tgcgcaggtc tcgcttggcc ttcaggtaga tggagctggc gtggcggttg
     6241 tgcagctcgt cgaggcgcgg cgtgacctcg cgccagatcc gctgctcctc gccggtgtag
     6301 tcgatgatcg gcggcggctc gttccggagc cgcgcctcgc gacagatcgc gaagagggca
     6361 cggcgccgct cgacgtact
//
LOCUS       DAOPQX010000205         6341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13434_length_6341_cov_4.550748, whole genome shotgun sequence.
ACCESSION   DAOPQX010000205 DAOPQX010000000
VERSION     DAOPQX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13434_length_6341_cov_4.550748"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKK95_11210"
     CDS             <1..357
                     /locus_tag="PKK95_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18831.1"
                     /translation="ATGVTANAAVPVTVFPAVPFTLEVAASSGRVNQPITITATAPPG
                     SPQINKFVWSLGGPLDPAIDPDGDGIVETSRPTVSIRYVGMPCNATTCVVQIQVTAEA
                     EQNRFGFGSTTTTITQ"
     gene            complement(608..1021)
                     /locus_tag="PKK95_11215"
     CDS             complement(608..1021)
                     /locus_tag="PKK95_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOC18832.1"
                     /translation="MVFTRLHARQRAAGFTLIELMVVMALITVLAGMAVVLYRNSIIT
                     SREAVLKTDLFRMRDAIDQYYADKAKYPADLQALVTDGYLREVPVDPFTNSRETWQTV
                     PAEIDPANPTTEVGIYDVKSGSENTALDGSKYSEW"
     gene            complement(1028..1528)
                     /locus_tag="PKK95_11220"
     CDS             complement(1028..1528)
                     /locus_tag="PKK95_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOC18833.1"
                     /translation="MSTKHDRPWITLRRQEGFSFVELLVVSALLLIISSAVLPLAKVT
                     IQRQREVELRRALREMRTAIDRYKDAVDQGMIGSVDVKAGSEGYPADLDALVNGVAVA
                     NDASGRKLKFLRRIPIDPMTKSTDWGKRSYQDSPESTSWGGQNVFDVYTKSGGTGLDG
                     TKYSDW"
     gene            complement(1546..4026)
                     /locus_tag="PKK95_11225"
     CDS             complement(1546..4026)
                     /locus_tag="PKK95_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOC18834.1"
                     /translation="MRVNLLRAAAVAVALVTLAGCGASRAYSRGERAARAGDWDTAVS
                     YYTQAFQANPRRTEYRIALERAQLAASLDHLDRARAFDEKGELEAAIREYRRAAEFDS
                     TNARAAARAIELERVLRDKLEAARPVPQIEQLREQVRQRQENVLNPASREPIRAKFVS
                     GIQLSEVLNFIGQASGINILYERDFQDRRLTNPIELDGVTLEQALHLVLTSNGFFYKV
                     QNPRTIMVIPDQPQKRAIYEEQVIQVFHVSNADVTELQTLLTGLFQGAGITARPLVTA
                     NKTSNTITMRGSAAMVAIAEKVIQANDKPRAEVVIDIEILEVNRSRAKAYGLNLSQYS
                     ITGVFSPEAPPGGTGTGTGTGTGTGTGTSNASGLFNLNSISQGINTADFYVGVPSWII
                     RFLESDSSSKLIAKPSLRGQEGKTLTANLGDEIPVPSTTFTPLIGGGTAVNPLTSFQY
                     RPVGVNVEVTPRVTYTNDIILDLTVESSTKGSDVNIAGQSLPSFGSRRVKTTMRLRDG
                     ESNLLAGLLRDDERRSLSGFPGAIHVPILKQLFSANDNQIAQTDIVMLLTPRIIRTHG
                     LTAEDVAPIYIGTTQNPSLGGGPPPLIEPGGAAEQPAIQPAPQAGAGGTPMGRPTPQG
                     TPVLPPGSSPIPGTVMQPPPQPVPPVAAPPAAPPPAAAEPAPAAQPPVAEPTPPAAAA
                     QPGAAQAGGAQVVVTPPSGEFRAGGGPYTVPLLVNNINRVSTVSLTLTFDPAVLRVRG
                     VQEGSFMRQGNANVAFAQQVDSASGRVDVTLSRSGDTVGATGSGLLAAVLFETVAPGS
                     TALTPAGVASGPGGPIRLDFVPAAVTVK"
     gene            complement(4178..4717)
                     /locus_tag="PKK95_11230"
     CDS             complement(4178..4717)
                     /locus_tag="PKK95_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18835.1"
                     /translation="MAAEVPRRQAVLLASLLAVLAAILAYQFWWSGDGRGPRGANARP
                     AARGAASGPMSEVPDVRLEALEAERPAPVDGGRNLFRDQPEPSPAPPPPDVPARRAAV
                     RPVPTGPPPPPPINLKFIGVVQAGGRSVAVLSDGRDILYGREGEVVDGQYLIVKINVE
                     TIEVSYPDGTGRRVIRLTG"
     gene            complement(4707..5288)
                     /locus_tag="PKK95_11235"
     CDS             complement(4707..5288)
                     /locus_tag="PKK95_11235"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HOC18836.1"
                     /translation="MAATGLLNRVLREERRPIVALAVAFALNLALYGLAVRPMSQRVT
                     DADARAAAAEQSRRAAAAEFSSVRAVAAGKDQAERELKVFYEQVLPASLSDAQRATYV
                     SLAQLARETNLRLSNRLASAEPAARASLDRLAISIALEGDYQDIRQFIYRVETGAPFL
                     VIDDLRLDGGASGETLRLSLNLSTYYQAAGNGS"
     gene            complement(5288..5875)
                     /locus_tag="PKK95_11240"
     CDS             complement(5288..5875)
                     /locus_tag="PKK95_11240"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOC18837.1"
                     /translation="MLRTNLATRPFYDVRRVHAVLAAAAVLVAAFTVFNAWQLFSLTR
                     RDASLAASIRQSDATAATLRQDAARARASIDRERLAAVLEASREANALIDRRVFSWTA
                     LLNQLETTLPAGVRIRSIRPVTGEVMRIELVARARRAEDVEAFVENLQGTGAFQDVFT
                     RSENTQEDGMIEVLLEGRYASPAPPAPDGGQSGGR"
     gene            complement(5883..>6341)
                     /locus_tag="PKK95_11245"
     CDS             complement(5883..>6341)
                     /locus_tag="PKK95_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18838.1"
                     /translation="GTRLKLMSLAVLAVLAAGLTEQGDWLLVHVTPGYGSIGILRGRD
                     LIFFRNRAEGTDETLADLVHQTAMYYEDRLGGSAKFARVLLAASGPCRDDAPSSLDVV
                     RRQVEERLRVKTQTFDPSAVARFTDRIAPDRDLLQAVTPLLGVLARGARE"
BASE COUNT          888 a         2215 c         2241 g          997 t
ORIGIN      
        1 gccacgggcg tcacggccaa cgctgctgtc ccggtgaccg tcttccccgc cgttccattc
       61 accttggagg tcgcagcgtc ctccggccgt gtgaaccagc ccatcacgat caccgccacg
      121 gctccgcccg gttcgccgca gatcaacaag ttcgtgtgga gcctcggtgg tccgctggat
      181 ccggcgatcg acccggatgg cgacggtatc gtggagacaa gccggcccac cgtgtcgatc
      241 cggtatgtcg gcatgccgtg caacgccacc acttgcgtag tccagattca ggtgactgcg
      301 gaggcggagc aaaaccgctt tgggttcggc agcacgacga cgacgatcac gcaatagcag
      361 gctgccgtga aagcgcgcct gtgcgggagt tcaccacagg ctgacaggcg gaccacgaag
      421 ttgggacgcg aaagggccgg cgcgatgcgc cggcccttcg tctattgccg tacggttcac
      481 cgcagaagcg cagagatctt tgcgatgatt ccgtgatctc tgcatacgtc tccgcgtctc
      541 ggtgtctccg cggtgaacgt ggacaagccg ccacgtctgt cacgcaggct tcaccgcagg
      601 ggccgtctca ccactcgctg tacttgctgc cgtccagcgc ggtgttctcc gatccgctct
      661 tgacgtcgta gatgccgacc tccgtggtgg gattggcggg atcgatctcg gcgggaacgg
      721 tctgccaggt ttcgcgcgag ttggtgaagg gatcgaccgg gacctcgcgc aggtagccgt
      781 cggtgaccag ggcctgcagg tcagccgggt acttcgcctt gtccgcgtag tactggtcga
      841 tcgcgtcgcg catccggaag agatcggtct tgagcaccgc ctccctggag gtgatgatcg
      901 aattgcggta gaggacgacg gccatcccgg cgaggacggt gatgagggcc atcaccacca
      961 tcagctcgat cagcgtgaat ccggccgcgc gttgccgggc gtgcaggcgc gtgaacacca
     1021 tgtcggccta ccagtcgctg tacttcgtcc cgtcgaggcc cgtcccgccg ctcttcgtgt
     1081 agacgtcgaa cacgttctgg ccgccccagg aggtcgactc gggcgagtcc tggtaggacc
     1141 gcttgcccca gtcggtgctc ttggtcatcg ggtcgatcgg gattcgcctc aggaacttca
     1201 gcttgcgccc cgacgcgtcg ttggcgacgg cgacgccgtt gaccagcgcg tccaggtcgg
     1261 ccgggtaccc ttctgaaccg gccttgacgt ccaccgagcc gatcatcccc tggtccaccg
     1321 cgtccttgta gcggtcgatg gcggtccgca tctcgcgcag cgcgcgccgc agttcgacct
     1381 cgcgctgccg ctggatcgtc accttcgcca gcggcagcac cgccgacgag atgatcagga
     1441 ggagcgcgct gacgacgagc agctcgacga agctgaagcc ctcttgccgt ctcagcgtga
     1501 tccacggcct gtcgtgcttc gtgctcaacc tctcgaaccc cttccctact tcaccgtgac
     1561 cgccgccggc acgaagtcga ggcggatcgg gccgcccggg ccgctcgcca cgccagccgg
     1621 cgtgagcgcc gtggatccgg gcgcgaccgt ctcgaacagg acggccgcca gcagccccga
     1681 gccggtcgcc cccaccgtgt cgcccgagcg gctgagcgtc acgtccaccc gccccgacgc
     1741 cgaatccacc tgctgggcga acgccacgtt ggcgttgccc tggcgcatga agctcccctc
     1801 ctgcacgccg cgcacgcgca agaccgcagg atcaaaggtc agcgtcaggc tcaccgtgga
     1861 cacgcggttg atgttgttca ccagcaacgg caccgtgtag ggcccgccac ccgcccggaa
     1921 ttccccgctt ggcggcgtca ccaccacctg cgccccgccg gcctgcgccg cgcctggttg
     1981 tgcggccgcg gccggcggtg tgggctcggc cactggcggc tgcgccgcgg gggcgggctc
     2041 ggcggccgcg ggcggcgggg ccgccggcgg agcggccacc gggggaacgg gctgcggcgg
     2101 cggctgcatg accgtccccg ggatcggcga cgagcctggc ggaagcaccg gggtcccctg
     2161 cggcgtcggc ctgcccatcg gcgtgccacc cgcacccgcc tggggcgccg gctggatggc
     2221 cggctgctcg gcggccccgc cgggctcgat gaggggcggg ggcccgccgc cgaggctcgg
     2281 gttctgggtg gtaccgatgt agatcggcgc cacgtcctcg gcggtcagcc cgtgcgtccg
     2341 gatgatgcgc ggcgtcagca gcatgacgat gtcggtctgc gcgatctggt tgtcgttcgc
     2401 cgagaagagc tgcttgagga tcggcacgtg gatcgcgccc gggaacccgc tgagcgaacg
     2461 ccgctcgtcg tcgcgcagca gcccggccag caggttcgac tcgccgtcgc gcagccgcat
     2521 ggtcgtcttg acgcggcgcg agccgaagga gggcaggctc tgccccgcga tgttgacgtc
     2581 gctccccttc gtgctgctct cgacggtgag gtcgaggatg atgtcgttgg tgtaggtgac
     2641 gcggggcgtc acctcgacgt tcacgccgac cgggcggtac tggaacgagg tcagcgggtt
     2701 gaccgccgtc ccgccgccga tcagcggcgt aaaggtcgtc gagggcaccg ggatctcgtc
     2761 gccgaggttc gcggtgagcg tcttgccctc ctggcctcgc agcgacggct tggcgatcag
     2821 cttgctcgac gagtcgctct cgaggaaccg gatgatccac gacgggacgc cgacgtagaa
     2881 gtcggcggtg ttgatgccct gcgagatgct gttcaggttg aacaggccgc tggcgttgga
     2941 cgtgcccgtg ccggtcccgg tccccgtacc ggtcccggtt cccgtgcccc cgggaggggc
     3001 ttcgggcgag aagacgcccg tgatcgagta ctgcgagagg ttcaggccgt aggccttcgc
     3061 ccggctccgg ttcacctcga ggatctcgat gtcgatgacc acctcggccc gcggcttgtc
     3121 gttggcctgg atcactttct cggcgatggc caccatcgcg gcgctgccgc gcatggtgat
     3181 ggtgttcgag gtcttgttcg ccgtcaccag ggggcgcgcc gtgatcccgg ctccctggaa
     3241 aagcccggtg agcagcgtct gcagctcggt cacgtcggcg ttcgagacgt ggaagacctg
     3301 gatcacctgc tcctcgtaga tcgcgcgctt ctgcggctgg tcggggatga ccatgatggt
     3361 gcgcgggttc tggaccttgt agaagaagcc gttcgaggtc agcaccaggt ggagggcctg
     3421 ctcgagggtg acgccgtcca gctcgatcgg gttcgtcagc cggcggtcct ggaagtcgcg
     3481 ctcgtagagg atgttgatcc ccgacgcctg gccgatgaag ttgagcacct ccgacagctg
     3541 gatccccgag acgaacttgg cccggatcgg ctcgcgggac gcggggttca gcacgttctc
     3601 ctgccgctgc cggacctgct cgcggagctg ctcgatctgc ggcacgggcc gggcggcctc
     3661 gagcttgtcg cgcagcacgc gctcgagctc gatggcgcgg gccgcggcgc gcgcgttggt
     3721 cgagtcgaac tcggcggccc ggcggtactc gcggatcgcg gcctcgagct cgcccttctc
     3781 gtcgaaggcg cgggcgcgat ccaggtggtc cagcgaggcg gcgagctgcg cccgctcgag
     3841 agcgatgcgg tactcggttc ggcgggggtt cgcctggaac gcctgcgtgt agtagctcac
     3901 cgcagtgtcc cagtcgcccg cccgtgccgc ccgctcgccg cggctgtacg ccctcgaagc
     3961 gccgcagccg gcgagcgtca ccaacgccac cgccaccgcc gcagctctca agaggttgac
     4021 tctcacgcga ggcctccaat cggttcgccg ccgcacgacc ctactcctgc cgaatcactg
     4081 ttccctgttc ctcgacccca gatccccccg actcctgact ctcgacccct gaatcctgaa
     4141 tcccgaaccc tgattcccga atcccgaatc ctacccctta ccccgtcagc cggatcaccc
     4201 gtcggccggt tccgtccggg tacgacacct cgatcgtctc gacgttgatc ttcacgatca
     4261 ggtactgccc gtccacgacc tcgccctcgc ggccgtacag gatgtcgcgc ccgtcgctga
     4321 gaacagcgac cgaccgcccg cccgcctgca ccacgccgat gaacttcagg ttgatcggcg
     4381 gcggaggtgg cggcccggtc ggaacgggac gaacggcggc ccgcctggcg ggcacgtcgg
     4441 gcggcggggg cgcgggcgac ggctcgggct ggtcgcgaaa gaggttgcgc ccgccatcca
     4501 ccggggctgg acgctcggct tcgagggcct cgagacggac gtcggggacc tcgctcatcg
     4561 gccctgaagc cgcaccccgc gcggccggcc tcgcgttggc cccgcgcggg ccgcggccat
     4621 ccccgctcca ccagaactgg taggcgagga tggccgcgag cacagccagc aggctcgcca
     4681 gcagcacggc ctggcggcgc gggacctcag ctgccattgc cggccgcctg gtagtaggta
     4741 gagaggttga gcgacaggcg cagcgtctct cccgacgcgc cgccgtcgag ccgaaggtcg
     4801 tcgatcacca ggaacggcgc cccggtctcg acgcggtaga tgaactgtcg gatgtcctgg
     4861 tagtcgccct cgagcgcgat cgagatggcg aggcggtcga gggaggcgcg ggccgcgggc
     4921 tcggccgagg ccaggcggtt cgacaggcgc aggttggtct cgcgcgcgag ctgggccagg
     4981 ctcacgtagg tcgcgcgctg ggcgtcggag aggctggccg gcagcacctg ctcgtagaac
     5041 accttcagct cgcgctcggc ctggtccttg ccggcagcga cggcgcggac cgaggagaac
     5101 tcggcggcgg ccgcgcggcg cgactgctcg gcggcggcgg cgcgggcgtc ggcgtcggtc
     5161 acccgctgcg acatggggcg gaccgccagc ccgtagagcg cgaggttcag cgcgaaggcc
     5221 accgccagcg cgacgatcgg ccggcgctcc tctctcagca cgcggttgag caggcctgtg
     5281 gccgccatca gcgcccgcct gactgcccgc cgtcgggggc gggcggggcg gggctggcgt
     5341 accgcccctc cagcagcacc tcgatcatcc cgtcctcctg cgtgttctcc gatcgcgtga
     5401 acacgtcctg gaacgctccg gtgccctgca ggttctcgac gaaggcctcg acgtcctcgg
     5461 cgcggcgggc gcgcgccacc agctcgatcc gcatgacctc gccggtcacg ggccggatgg
     5521 atcggatccg gaccccggcg ggcagcgtgg tctcgagctg gttgagcagc gccgtccagg
     5581 agaagacgcg gcggtcgatc agcgcgttcg cctcgcgcga ggcctcgagc accgccgcca
     5641 ggcgctcgcg gtcgatgctg gcccgcgcgc gtgccgcgtc ctgccgcaag gtggccgcgg
     5701 tggcgtccga ctgccggatg gaggcggcca gcgacgcgtc gcggcgcgtg agcgagaaga
     5761 gctgccacgc gttgaacacc gtgaaggcag ccaccaggac ggcggcggcg gcgaggacgg
     5821 cgtgcacgcg ccgcacgtcg tagaagggac gggtcgcgag gttggtcctc agcatggcgg
     5881 cgtcactcgc gggccccgcg ggccagcacg ccgagcaggg gcgtcacggc ctggagcagg
     5941 tcgcggtcgg gcgcgatccg atcggtgaac cgcgcgacgg ccgaggggtc gaaggtctgg
     6001 gtcttcaccc gcagccgctc ctcgacctgg cggcggacga cgtcgagcga cgaaggggcg
     6061 tcgtcccggc acgggccgct cgcggcgagc aggacacggg cgaacttcgc gctcccgcca
     6121 aggcggtcct cgtagtacat cgccgtctgg tgcacgaggt cggccagcgt ctcgtcggtg
     6181 ccttcggctc ggttccggaa gaatatcaaa tcccggccgc gcaggatccc gatgctgccg
     6241 taacccgggg tcacgtgcac cagcagccag tcgccctgct cggtcaggcc ggcggcgagc
     6301 acggccagca cggcaaggct catcagcttg aggcgcgtgc c
//
LOCUS       DAOPQX010000206         6338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13445_length_6338_cov_4.807258, whole genome shotgun sequence.
ACCESSION   DAOPQX010000206 DAOPQX010000000
VERSION     DAOPQX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13445_length_6338_cov_4.807258"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PKK95_11250"
     CDS             complement(<1..368)
                     /locus_tag="PKK95_11250"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOC18839.1"
                     /translation="MNRLITLVGTAVVAGAVWSSPAEAQLRSAAVGGDDRAPRVARAA
                     SGQIEGAVADEQGRPLAGVMVSALGQTTALAVTDRAGAFKLQSLPPGGYLVRAHLAGF
                     APSRRELVQVGAGRPARFSVT"
     gene            complement(418..1488)
                     /locus_tag="PKK95_11255"
     CDS             complement(418..1488)
                     /locus_tag="PKK95_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18840.1"
                     /translation="MLIRARAVAVSGALLAAFALAVPAGTAAQDKKKEQKLDKDQQRE
                     VQTVVKIVDEVVAGQPAPTDFPITFQYHFQKARDQRTFMPVTMVFPQDRITNTSVVYY
                     VRVVPRGGAATAPEGNGEKDAKQDEKKGRPEYPFEDVRFFDLKAPENGQYRVTRAISV
                     PPGEFDLYVLMGERTPKDKKAEAPKRSLVKQAVTVPDFWANELMTSSIILSNKAEQLS
                     APPSEADMAANPYVFGLTRLTPSLDHKFSKKDELSIIFLIYNTAAAQGGKPDVKVEYN
                     FHQKQAGGGEKFFNRTEPQNFNAQTLPPQFDAAAGHQLVGGLSIPLASFPEGDYRLEI
                     KITDVAGNNKTKTENVDLSIAP"
     gene            1664..2989
                     /gene="glgC"
                     /locus_tag="PKK95_11260"
     CDS             1664..2989
                     /gene="glgC"
                     /locus_tag="PKK95_11260"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOC18841.1"
                     /translation="MVDDALVIVLAGGAGERLYPLTQDRAKPAVYFGGPYRIIDFVLS
                     NCINSGLRRIFIATQYKSLSLNRHIRMGWNIVSEELGEFIEILPPQKRVGEHWYLGTA
                     DAVFQNFYSILREHPRQVIVLAGDHVYKMDYSKMLRFHQEQGADVTLATIEVPIEESK
                     RFGIVRVDEHDRVVGFREKPHDPIPAPGTTDVALASMGIYVFEVDALREALEADAAQE
                     TTHDFGRDIIPALIPRARVCSYRFYDENKKAAKYWRDIGTLDAYFDASMDLVQVNPEF
                     NMYDPEWPLRTYQPQSPPAKFVFAEPGGRCGQALDSIISPGCIVSGSRVSGSVLCPNV
                     RVHSFCVIDQAILMPGVRVGRHARLRRVIVDRDVFIPRGARIGFDEEEDRRRHTVTKR
                     GVVVVTRDDEPLIGDIDPEALRLESEADSRGQEQQQGGVSREDSARARS"
     gene            2961..4292
                     /gene="eno"
                     /locus_tag="PKK95_11265"
     CDS             2961..4292
                     /gene="eno"
                     /locus_tag="PKK95_11265"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOC18842.1"
                     /translation="MKIARVHGREILDSRGNPTVEVEVTLEGGVQGRAAVPSGASTGE
                     REALELRDGDSKRFLGKGVLKAVSHVNGELASAVTGAEFDQRALDRRMIELDGTPTKS
                     RLGANAVLGISMAAAHAESRRQGIPLYRYLAGLAGQPADAGLLPVPMMNILNGGAHAD
                     SNVDVQEFMVVPLGLPSFREALRAGAETFHALRSILKQRGLSTGVGDEGGFAPNLQAN
                     HQAIELVIEAIGKAGYQAGKDVCIALDVAASELWEDEGSREGAPEARRGRYVFHKSGG
                     GAKTSAEMVEMYGEWIRQYPIVSIEDGLAEGDWSGWELLTRELGGRVQLVGDDVFVTN
                     PGILKRGIERKIANSILIKLNQIGTVTETLDAIEMARAAGYTSVISHRSGETEDSTIA
                     DLAVGTAAGQIKTGSASRSDRVAKYNQLLRIEEALGASARYAGREAFKQLA"
     gene            complement(4296..4589)
                     /locus_tag="PKK95_11270"
     CDS             complement(4296..4589)
                     /locus_tag="PKK95_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18843.1"
                     /translation="MNEEMIDRIGRELMAAIEMALAADPRVQACLDQARSAGIGFRVA
                     LNRARAQSGVPVEGAPAQAAPAGAARGLSAPAYSVTESDRRFLRSLRIAADDE"
     gene            4809..5789
                     /locus_tag="PKK95_11275"
     CDS             4809..5789
                     /locus_tag="PKK95_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18844.1"
                     /translation="MADPPPLLDVSHLTTVFDGPSGALVAVDDVSLKVGAGETVALVG
                     ESGSGKSVTALSILRLIEPPGRIAAGEILFRGRDLRSLGEEEMRQVRGREIGLVFQEP
                     ARALNPVFTIGAQVAETFEVHRLAAGREARARAIEMLAAAGIPDAADRARDYPHQLSG
                     GLLQRAAIAAALACRPALAIADEPTSSLDLGLQAQVLDLLARLRASFGLSLLLITHDL
                     DVASRVADRVAVMYSGCIVEEGPSGVVLHAPAHPYTAGLIAALPNGAPGRLRALDGSV
                     PPLGPRSPGCAFAPRCGRATPECQARRPETARLDTGHTVACYHPQGAVRA"
     gene            5782..>6338
                     /locus_tag="PKK95_11280"
     CDS             5782..>6338
                     /locus_tag="PKK95_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOC18845.1"
                     /translation="MPDDAPLLRVRHLVKEYTRRRGMLLRASTVRAVDGVSFEVGRGE
                     TLGLVGESGSGKTTTARMVLRLIEPTSGEVWFEGRDVLALPAGELRRLRRQMQMVFQD
                     PWSSLNPRMRVGAIVEEPLVIHRVGDRAARRERVVELLRLVGLDPSQARRYPSELSGG
                     QRQRIGLARALALEPSFLVADEPVSA"
BASE COUNT          965 a         2230 c         2195 g          948 t
ORIGIN      
        1 gtgacggaga agcgggcagg gcggcccgca ccgacctgca ccagctcgcg gcgagagggg
       61 gcgaagcccg ccaggtgggc ccgcacgaga tacccaccag gcggaagcga ctgcagcttg
      121 aacgcgccgg cccggtcggt caccgccagg gccgtcgtct ggccgagggc ggacaccatc
      181 acgccggcaa gcggccggcc ctgctcgtcg gcgaccgcgc cttcgatctg gccggacgcc
      241 gccctcgcca cgcggggcgc acggtcgtcc ccgccgacgg cagcgctccg gagctgcgcc
      301 tcggccggcg aggaccacac tgcgccggcc acgaccgccg tcccgacgag cgtaatgaga
      361 cggttcatga cgaggccttc agttgtgaag cacgagcacg ccacccggcg gccccggcta
      421 cggggcgatc gacaggtcga cgttctcggt cttcgtcttg ttgttacccg cgacgtcggt
      481 aatcttgatc tccagccggt agtcgccctc ggggaagctc gccagcggga tcgagaggcc
      541 gcccaccagc tggtggccgg ccgcggcgtc gaactgcggc ggcagcgtct gcgcgttgaa
      601 gttctgcggc tcggtgcggt tgaagaactt ctcgccgcca ccggcctgct tctggtggaa
      661 gttgtactcc accttcacgt cgggcttccc gccctgcgcg gccgccgtgt tgtagatcag
      721 aaagatgatc gacagctcgt ccttcttcga gaacttgtgg tcgagcgacg gggtcaggcg
      781 cgtcagcccg aagacgtacg ggttggccgc catgtcggcc tcggagggcg gcgcgctcag
      841 ctgctcggcc ttgttcgaca ggatgatcga gctggtcatc agctcgttcg cccagaagtc
      901 gggcacggtc acggcctgct tgacgaggga gcgcttcggc gcctcggcct tcttgtcctt
      961 cggcgtgcgc tcacccatga gcacgtagag gtcgaactcg ccgggcggca ccgagatcgc
     1021 gcgggtcacc cggtactgac cgttctcggg agccttcagg tcgaagaacc ggacgtcctc
     1081 gaacgggtac tccgggcgcc ccttcttttc gtcctgcttc gcgtccttct ccccattgcc
     1141 ctcgggagcg gttgccgcac cgccccgcgg cacgacccgg acgtagtaca ccaccgaggt
     1201 gttggtgatc cgatcctgcg ggaagaccat cgtcaccggc atgaaggtgc gctgatcgcg
     1261 ggctttctgg aagtggtact ggaacgtgat cggaaaatcg gtcggggccg gctgcccagc
     1321 caccacctcg tcgacgatct tcacgactgt ctggacctcg cgctgctggt ccttatccag
     1381 tttctgttcc ttcttcttgt cctgcgcggc ggttccggcg ggaacggcaa gggcgaacgc
     1441 ggcgagcagc gcaccggaaa cggctaccgc gcgggcacgg atcagcatga cgactctacc
     1501 cccaaatata gacgaaccat caatctacgg gatcatagac accttgcccg gccaaagtca
     1561 aaagagcgcg cggggccaag cccggccgct cggccgccgc gtgctatgat cggcagtccg
     1621 agcggcaaag caggcgtccg gggcggtcgc ccgggagagt ccgatggtcg acgacgcgct
     1681 cgttatcgtg ctcgcggggg gggccgggga gcgcctctac ccgctgaccc aggaccgggc
     1741 caagccggcc gtctacttcg gcgggcccta ccgcatcatc gacttcgtcc tcagcaactg
     1801 catcaactcg ggcctgaggc gcatcttcat cgccacccag tacaagtcgc tctcgctcaa
     1861 ccgccacatc cggatgggct ggaacatcgt ctcggaggag ctcggggagt tcatcgagat
     1921 cctccccccg cagaagcggg tcggggagca ctggtacctc ggcaccgccg acgccgtctt
     1981 ccagaacttc tactcgatcc tccgcgagca cccccgccag gtcatcgtcc tcgccggcga
     2041 ccacgtctac aagatggact actccaagat gctccggttc caccaggagc agggggccga
     2101 cgtgacgctg gcgaccatcg aggtcccgat cgaggagtcg aagcggttcg gcatcgtgcg
     2161 cgtggacgag cacgaccgcg tcgtcggctt cagggaaaag ccgcacgacc cgatccccgc
     2221 gcccggcacg accgacgtcg cgctcgcgtc gatgggcatc tacgtcttcg aggtggatgc
     2281 cctgcgagag gcgctcgagg ccgacgccgc ccaggagacg acgcacgact tcgggcgcga
     2341 catcatcccg gcgctcattc cccgcgcgag ggtctgctcc taccggttct acgacgagaa
     2401 caagaaggcc gcgaagtact ggcgcgacat cgggacgctc gatgcctact tcgacgcgag
     2461 catggacctc gtgcaggtga accccgagtt caacatgtac gaccccgagt ggccgctgcg
     2521 cacctaccag ccgcagtcgc cgccggccaa gttcgtcttc gccgagcccg gggggcgctg
     2581 cggccaggcg ctcgactcga tcatctctcc cggctgcatc gtctcgggca gccgggtatc
     2641 gggcagcgtg ctgtgcccca acgtccgcgt ccacagcttc tgcgtcatcg accaggcgat
     2701 cctgatgccc ggcgtccgcg tcgggcgtca cgcccgcctg cggcgcgtca tcgtggaccg
     2761 cgacgtcttc atcccccgcg gcgcccgcat cgggttcgac gaggaggaag accggcgccg
     2821 gcacaccgtc accaagcgcg gcgtggtcgt cgtgactcgc gacgacgagc cgctcatcgg
     2881 ggacatcgac cctgaggcgc tgcggctcga gtccgaggcg gacagccgcg gccaggaaca
     2941 gcagcaagga ggagtgagtc gtgaagatag cgcgcgtgca cggtcgtgaa atcctggatt
     3001 cgcggggcaa cccgacggtc gaagtcgagg tgacgctcga gggcggggtg caggggcggg
     3061 cggccgtccc ctcgggggca tccaccggcg agcgcgaggc gctcgagctc cgcgacggcg
     3121 acagcaagcg gttcctcggc aagggcgtcc tcaaggcggt gtcgcacgtc aacggcgagc
     3181 tggcctcggc ggtgaccggc gctgagttcg accagcgggc gctcgaccgg cggatgatcg
     3241 agctcgacgg aacccccacc aagagccggc tcggggccaa cgccgtgctc ggcatctcca
     3301 tggccgccgc gcacgccgag tcccggcgac aggggatccc cctctaccgc tacctggccg
     3361 gcctggccgg gcagcccgcg gacgccgggc tgctccccgt gccgatgatg aacatcctca
     3421 acggcggcgc ccacgccgac agcaacgtcg acgtccagga gttcatggtc gtcccgctcg
     3481 ggctgccgtc gttccgcgag gcgctccgcg ccggggccga gacgtttcat gccctgcgct
     3541 cgatcctgaa gcagcggggg ctctcgaccg gcgtcggcga cgaaggaggc ttcgcgccga
     3601 acctccaggc caaccaccag gccatcgagc tggtgatcga ggccatcggc aaggccggct
     3661 accaggccgg caaggacgtg tgcatcgcgc tcgacgtggc cgccagcgag ctgtgggagg
     3721 acgaggggag ccgcgagggg gcgcccgagg cgcgccgcgg ccgctacgtc ttccacaagt
     3781 cgggcggggg cgccaagacc tcggccgaga tggtcgagat gtacggcgag tggatccggc
     3841 aatacccgat cgtgtcgatc gaggacggcc tggccgaggg tgactggtcg ggctgggaac
     3901 tgctcacgcg cgagctcggc gggcgcgttc agctcgtcgg cgacgacgtc ttcgtcacca
     3961 accccggcat cctgaagcgc ggcatcgagc gcaagatcgc caacagcatc ctcatcaagc
     4021 tcaaccagat cgggaccgtc accgagacgc tcgacgccat cgagatggcg cgcgcggccg
     4081 gctacacctc ggtcatctcg caccgctcgg gcgaaaccga ggacagcacg atcgccgacc
     4141 tcgccgtcgg caccgcggcc ggccagatca agaccggatc ggccagccgg tccgacaggg
     4201 tggcgaagta caaccagttg ctgcggatcg aggaagcgct gggagcgtcg gcgcggtacg
     4261 ccgggcgcga ggcgttcaag cagctcgcgt agggactact cgtcgtccgc cgcaatcctg
     4321 agcgacctga ggaagcgccg gtcggactcg gtcaccgagt aggccggcgc ggacaacccg
     4381 cgggcggcgc cggccggcgc cgcctgcgcg ggcgcaccct ccacgggcac gccgctctgg
     4441 gcccgcgccc ggttcagcgc gactcgaaac ccgattcccg ccgatctggc ctggtcgagg
     4501 cacgcctgca cacgcgggtc tgcagcgagc gccatctcga tggccgccat cagctcccgc
     4561 ccgatcctgt cgatcatctc ttcgttcatg tccacttggt agggaaagac ggcgatcccg
     4621 gccggtcgcc aggatgcagc ccggccccac tcctgtcggg gctttgcctt ccgcttgtag
     4681 tagagggaat cgcgaagtgc ggttctgagc gcatcttacg ggcacggccc gcaggtgtca
     4741 aggaaatgac ggcctgggcg catccgcagg cgacccggac acgcccggcg gactacaatg
     4801 ggcggccaat ggccgaccct ccgccgctgc tcgacgtcag ccacctcacc acggtgttcg
     4861 acggcccgtc cggggcgctc gtcgcggtcg atgacgtcag cctgaaggtc ggcgccggag
     4921 aaaccgtcgc gctcgtggga gagtcgggca gcgggaagtc ggtgacggcg ctctccattt
     4981 tgcgcctcat cgagccgccg ggacggatcg cggcggggga aatcctcttt cgcggccgcg
     5041 acctccggtc tctcggcgaa gaggagatgc gccaggtccg tggccgtgag atcggcctgg
     5101 tcttccagga gcctgcgcgg gcgttgaacc cggtcttcac catcggggcc caggtggccg
     5161 agacgttcga ggtgcatcgc ctggcagcgg ggcgggaggc tcgggcgagg gcgatcgaga
     5221 tgctggcggc cgccggcatc ccggatgccg ccgaccgtgc gcgggactac ccgcaccagt
     5281 tgtcgggggg gctgctgcag cgggccgcca tcgccgccgc gctggcgtgc cggcctgcgc
     5341 tggccatcgc cgacgagcca acctcgtcgc tcgacctggg ccttcaggca caggtgctcg
     5401 acctgctcgc ccggctgcgc gcgtcgttcg gcctctcgct gctcctcatc acccacgacc
     5461 tcgacgtggc ctcgcgcgta gccgaccgcg tcgcggtcat gtactcggga tgcattgtcg
     5521 aggagggccc ctccggggtc gtgcttcacg cccccgcgca cccgtacacg gcagggctga
     5581 tcgcggcctt gccaaacggg gcgccggggc gcctccgcgc cctcgacggc tcggttccgc
     5641 cgctcggccc acgcagcccg ggatgcgcct tcgccccccg ctgcggcagg gcgacgccgg
     5701 agtgccaggc gcgacggccc gaaaccgcgc ggctcgacac cggccacaca gtggcctgtt
     5761 accaccccca gggggcggtc cgtgcctgac gacgccccgc tgctgcgcgt gcgccacctc
     5821 gtcaaggagt acacgcgccg gcgagggatg ctgctgcggg cctcgacggt tcgcgcggtg
     5881 gacggcgtga gcttcgaggt gggccggggt gaaacgctgg gcctggtcgg ggagtcgggc
     5941 agcggcaaga cgacgaccgc ccggatggtg ctccgcttga tagagccgac ttcgggcgag
     6001 gtctggttcg aaggccggga cgtgctggcc cttcccgccg gcgagctgcg tcggttgcgc
     6061 cggcagatgc agatggtctt ccaggatccc tggtcgtcgc tcaacccgcg gatgcgtgtg
     6121 ggggcaatcg tcgaagagcc gctggtcatc caccgcgtcg gcgaccgcgc ggcgcggcgg
     6181 gagcgcgtgg tggaactgct gcgcctcgtt ggcctcgatc cttcccaggc tcgccgctac
     6241 ccctccgagc tgagcggcgg ccagcgccag cgcatcggcc tggcccgcgc gctcgcgctg
     6301 gagccctcgt tcctcgtcgc cgacgaaccg gtgtcggc
//
LOCUS       DAOPQX010000207         6307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13546_length_6307_cov_4.832054, whole genome shotgun sequence.
ACCESSION   DAOPQX010000207 DAOPQX010000000
VERSION     DAOPQX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13546_length_6307_cov_4.832054"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKK95_11285"
     CDS             <1..416
                     /locus_tag="PKK95_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18846.1"
                     /translation="SIGPLRGGPAGVRLIEPTRGDALYVGDVPPPFEPPRRPGDDFYL
                     VAAGQVFGSDELSQRADPIAGRMHAPFVALGAEDAGAAKWEPGVEVDVELGARVLRLP
                     VAIWPGLPRGVAALPVGLGALRGLGLPAWGRLRLR"
     gene            392..1354
                     /gene="nuoH"
                     /locus_tag="PKK95_11290"
     CDS             392..1354
                     /gene="nuoH"
                     /locus_tag="PKK95_11290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOC18847.1"
                     /translation="MGAPTSSVIALLIVFGVLGSALTAAAVLIWAERRLLALFQDRYG
                     PNRVGPFGLLQVVADMIKIFTKEDWIPPFADRVVFVLAPAVIVGSVLASFAVLPIVPG
                     VVVADLNVGVLFFLAMSSLAVYGVVLAGWASNNKYSLLGGLRAASQMISYEVFLGLSL
                     MGVVAIAGSFSLVDIVEAQRGTWFVVPQFLGFVLFVVAASAESRRAPFDLPEAESELI
                     AGFHVEYSGMKFGMFYVAEYMSLWLLSAFVTVLFFGGWLGPVLPPAAWFLLKTIFFVV
                     FFVLVRASLPRPRFDQLMGFAWKVMLPLALANLVVTGAIVLARG"
     gene            1405..1959
                     /gene="nuoI"
                     /locus_tag="PKK95_11295"
     CDS             1405..1959
                     /gene="nuoI"
                     /locus_tag="PKK95_11295"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOC18848.1"
                     /translation="MRLDGREYIGRIPGVGVLQTLWNVFLHAFKPRFTVQYPEEKVPL
                     PPRWRGRIVLTRDQAGEERCVACYLCSAACPVDCIALQATEDESGRRYPAWFRINFSR
                     CIFCGFCEEACPTYAIQLTPDMEMSEYDRQNMVYEKEDLLISGPGKYPDYDYWKVAGR
                     EIGGKPKGAAEREQPPADLHDLRP"
     gene            1967..2566
                     /gene="nuoJ"
                     /locus_tag="PKK95_11300"
     CDS             1967..2566
                     /gene="nuoJ"
                     /locus_tag="PKK95_11300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOC18849.1"
                     /translation="MQAAFYLAGAVAVASTVLMLTRLNVVHALLFLVLSLIAVAVAFF
                     ALGAPLVGALEIIVYAGAIVVLFVFVVMMLNLGDEASEQERRWLAPGIWRLPLALGAV
                     LLAGAVALVLGGSGAGVVQPVAPKEVAVSLFGPYAIGVELASMLLLAGLVGAYHLGWQ
                     RRAQRPEEEVRDGLDTGGARVAAGRDTVRPGPRRPPRAA"
     gene            2508..2789
                     /gene="nuoK"
                     /locus_tag="PKK95_11305"
     CDS             2508..2789
                     /gene="nuoK"
                     /locus_tag="PKK95_11305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004881318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOC18850.1"
                     /translation="MLLAATLFALGLVGLLVRRDVVFMLMSLEVMLNAAGLAFVAAGG
                     GWGQPDGQVMFLFVLSVAAAEVAVGLALVLRLYKRTLSLDADAASRLRG"
     gene            2791..4683
                     /gene="nuoL"
                     /locus_tag="PKK95_11310"
     CDS             2791..4683
                     /gene="nuoL"
                     /locus_tag="PKK95_11310"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOC18851.1"
                     /translation="MYDLLWLVPALPALGFLALALAGPRLPRAAVAVIGAGSVGASAA
                     VTLGLAGAFLASPPPGHAFTQRLWTWMAAGGFQADFTLRLDPLSLLMLVVITFVGFLI
                     HVYSVEFMWDDDGFARFFAYMNLFVASMATLVLGANLLLLYLGWEGVGLCSYLLIGFW
                     YKDPANGRAGRKAFVVTRVGDTAMAIGLLLLFRELGTLDIAELLRRAAATWEPGSTMA
                     IAAAALLLGGALGKSAQLPLQVWLPDAMAGPTPTSALIHAATMVTAGVYLIARTHVLF
                     ELAPAVQLAVACVGAATLLLAGCSALAQRDIKRILAYSTVSQIGYMFLALGVGAWTAA
                     MFHFMTHAFFKALLFLAAGIVIEALHHEHDIFRMGGLRRRLPIAFWAFAAGGAALAGL
                     PLVSSGFYSKDAIVWAAYAGPLGNGVFWALAALGVLVTSLYTFRLIFVVFFGEPRGEI
                     GHRPGWAMSVPVAILAVLSVVAGFLNVPPTLGGIHLFDDFLAPVLPAMAHHEGGGFTE
                     AASQVLTALAFGLGLAAAWVLFLRRRDAAARIAASPPGHAVGRLWREGWGFDWLYERV
                     LVRPITALASAGRGDFVDGPFEAAGRAAFALHVAASATQTGRLRWYAAGLAGGSLAAV
                     AIALFL"
     gene            4680..6224
                     /locus_tag="PKK95_11315"
     CDS             4680..6224
                     /locus_tag="PKK95_11315"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOC18852.1"
                     /translation="MILLLLVLVPLAGAVLAWLVERLGAAWPRVVSLAALGGCFGLTA
                     AAWLGQASRASLPAVAGPGPWLLEFDAAWLPSIGARFHLAMDGLSLPLVGLTFFLGLA
                     GVIASWTEIRERVGFFHFNLLAVLAGVTVVFLAADLFLFYFGWELMLVPMYFLIAIWG
                     HEGRVHAAIKFFLFTQISGLLMLVALLALYLAHGAQTGLYTFEYRELLGTVLPERTEW
                     WLMLGFFIAFAVKLPVFPLHTWLPDAHTQAPTAGSVILAGLLLKTGGYGLIRFVVPLF
                     PGAAADFAPVAMTLAVAGILYGAILALGQTDLKRLVAYTSVSHLGFVLLGVFAWNTLA
                     LQGALLQMVAHGISTGALFVVAGLLQERMHTRDIDRMGGLWSVAPRLGGVALFFALAS
                     LGLPGLGDFAAEFLVLLGAFQASAVAASVAALGMVASALYSLRLVQRAFQGTNTNGWR
                     IPDLVPREALLFAPMVAVLVWLGLYPQPVIDTFRPVAERLQQATTLPVNAEGRPEAVL
                     GAEVGQ"
BASE COUNT          648 a         2201 c         2282 g         1176 t
ORIGIN      
        1 gctcgatcgg cccgcttcgc ggcggccccg ccggcgtccg gctgatcgag ccgacgcgcg
       61 gggacgccct ctacgtcggg gacgtgccgc cgccgttcga gccgccccgc cgcccggggg
      121 acgacttcta cctggtggcg gcgggccagg tgttcggctc cgacgagctc agccagcggg
      181 ccgacccgat cgccgggcga atgcacgcac cgttcgtggc gctcggcgcg gaggatgcag
      241 gcgcggccaa gtgggagccg ggcgtggagg tggacgtcga gttgggcgcg cgcgtgttgc
      301 gcctcccggt tgcgatttgg ccgggccttc cgcgcggcgt ggcggcgctg ccggtgggcc
      361 tcggcgcgct gcgcggcctc gggctgccgg catgggggcg cctacgtctt cggtgatcgc
      421 cctcctcatc gtcttcggcg tgctcgggag cgcgctcacg gccgcggcgg tgctgatctg
      481 ggccgagcgg cggctgctcg cactgttcca ggatcgctac ggccccaacc gggtcggccc
      541 gttcggcctg ctccaggtcg tcgccgacat gatcaagatc ttcaccaagg aggactggat
      601 cccccccttc gccgaccggg tggtgttcgt cctggcgccg gcggtgatcg tgggaagcgt
      661 gctcgcgtcg tttgccgtgc tgccgatcgt gcccggcgtg gtagtggccg acctcaacgt
      721 cggcgtgctc ttcttcctcg cgatgtcgtc gctggccgtg tacggggtgg tgctcgcggg
      781 gtgggcctcc aacaacaagt actcgctgct cggcgggctt cgcgcggcgt cgcagatgat
      841 cagctacgag gtcttcctcg gcctgtcgct gatgggcgtg gtcgccatcg ccggctcctt
      901 cagcctggtg gacatcgtgg aggcgcagcg cggcacctgg ttcgtcgtcc cgcagttcct
      961 gggcttcgta ctgttcgtcg tcgcggcgtc ggccgaatcg cggcgggcgc ccttcgatct
     1021 gcccgaggcc gagagcgagc tgattgccgg gttccacgtc gagtactcgg ggatgaagtt
     1081 cgggatgttc tacgtggccg agtacatgag cctctggctg ctgtcggcgt tcgtcaccgt
     1141 gctgttcttc ggcgggtggc tggggcccgt cctgccgccc gccgcctggt tcctgctgaa
     1201 gacgatcttc ttcgtcgtgt tcttcgtcct cgtgcgggcc tcgctcccga ggccgcggtt
     1261 cgatcagctg atgggattcg cgtggaaggt gatgctgccg ctcgccctgg ccaacctcgt
     1321 cgtgaccggg gcgatcgtgc tggcacgggg ttgaggcagc ggtgggggct gcagggtggc
     1381 cgcccggcgg aggggagaac ggggatgcga ttggacggtc gtgagtacat cgggcggatc
     1441 ccgggcgtcg gggtgctgca gacgctctgg aacgtgttcc tccacgcctt caagccccgc
     1501 ttcaccgtcc agtacccgga ggagaaggtg ccgctgccgc cccgctggcg gggtcggatc
     1561 gtcctgacgc gcgaccaggc gggcgaggag cgctgcgtcg cgtgctacct ctgctcggcc
     1621 gcctgcccgg tggactgcat cgcgctccag gccaccgagg acgagtcggg gcgacggtac
     1681 ccggcgtggt tccggatcaa cttctctcgc tgcatcttct gcggcttctg cgaggaggcg
     1741 tgcccgacct acgcgattca gctcacgccc gacatggaga tgagcgagta cgaccggcag
     1801 aacatggtct acgagaagga ggacctcctc atctcgggcc cgggcaagta tcccgactac
     1861 gactactgga aggtcgccgg gcgggagatc ggcggcaagc ccaagggcgc cgccgagcgc
     1921 gagcagccgc cggccgatct ccacgacctg cggccctgag gaaccgatgc aggcagcctt
     1981 ctacctggcc ggtgccgtcg cggtggcctc cacggtgctg atgctgacgc gcctgaacgt
     2041 cgtgcacgcg ctgctcttcc tcgtgctgtc gctcatcgcg gtcgccgtgg cgttcttcgc
     2101 gctcggcgcg ccgctggtcg gcgcgctcga gatcatcgtg tacgcgggcg ccatcgtggt
     2161 gctcttcgtc ttcgtcgtga tgatgctgaa cctgggggac gaggcgtccg agcaggagcg
     2221 gcgatggctg gccccgggga tctggcgcct cccgctcgcg ctcggcgcgg tgctgctggc
     2281 gggggcggtc gccctcgtgc tcggcgggag cggggccggc gtggtgcagc ctgtcgcgcc
     2341 gaaggaggtg gccgtgtccc tgttcgggcc gtacgcgatc ggggtcgagc tggcctcgat
     2401 gctgctgctg gccggcctgg tcggcgccta ccacctcggg tggcagcgcc gcgcgcagcg
     2461 gccggaggaa gaggtgcggg atgggctcga taccggtgga gcacgggttg ctgctggccg
     2521 cgacactgtt cgccctgggc ctcgtcggcc tcctcgtgcg gcgtgacgtg gtcttcatgc
     2581 tgatgtcgct cgaggtgatg ctcaacgcgg ccggcctggc cttcgtcgcg gccggcggag
     2641 gctgggggca gcccgacggc caggtgatgt tcctcttcgt gctgtcggtc gccgccgcgg
     2701 aggtggcggt cgggctggcg ctggtgctgc gcctctacaa gcggaccctg agcctggacg
     2761 ccgacgcggc gagccggctg cgaggctgac gtgtacgacc tgctctggct cgttccggcg
     2821 ctgcccgccc ttggcttcct ggcgctggcg cttgccggcc cgcgccttcc gcgcgcggcc
     2881 gtcgcggtca tcggcgcggg ctcggtgggc gcgtcggccg ccgtcacgct cggcctggcc
     2941 ggcgcctttc tcgcctcgcc gccgcctggc cacgcgttca cccagcggct gtggacctgg
     3001 atggccgccg ggggcttcca ggccgacttc acgctgcgcc tcgacccgct gtcgctgctg
     3061 atgctggtgg tcatcacctt cgtcggcttc ctcatccacg tctactcggt ggaattcatg
     3121 tgggacgacg acgggttcgc gcggttcttc gcgtacatga acctgttcgt ggcgtcgatg
     3181 gcgacgctcg tgctcggcgc caacctgctt ctcctgtacc tcgggtggga gggcgttgga
     3241 ctctgcagct acctgctcat tggcttttgg tacaaggatc cggcaaacgg ccgtgcgggg
     3301 cggaaggcgt tcgtggtcac ccgcgtgggc gacaccgcga tggcaatcgg cctgctgctc
     3361 ctcttccgcg agcttgggac gctggacatc gcggagctgc tgcgccgtgc cgctgcgacc
     3421 tgggagcctg gcagcacgat ggccattgcg gcggccgcgc tcctgctcgg gggcgccctc
     3481 ggcaagtcgg cgcagctgcc gctgcaggtc tggctgcccg acgcgatggc cggcccaacg
     3541 ccgacgagcg cgctgatcca cgcggccacc atggtgaccg ccggggtgta cctgatcgcg
     3601 aggacgcacg tcctgttcga gctggcgccg gcggtgcagc tggctgtcgc ctgcgtcggc
     3661 gccgcgacgc tgctcctggc cggctgcagc gcgctggccc agcgggacat caagcggatc
     3721 ctcgcctact cgaccgtcag ccagattggc tacatgttcc tcgcgctcgg cgtcggcgcg
     3781 tggacggccg cgatgttcca cttcatgacg cacgccttct tcaaggcgct gctgttcctc
     3841 gccgctggca tcgtgatcga ggcgctgcac cacgagcacg acatcttcag gatgggcggc
     3901 ctgaggcgga ggctgccgat cgcgttctgg gccttcgcgg ccggcggggc ggcgctggcc
     3961 gggcttccgc tggtcagttc gggcttctac agcaaggacg ccatcgtgtg ggcggcctac
     4021 gcgggcccgc tcggcaacgg cgtcttctgg gcgctggcgg ccctcggcgt cctcgtcacc
     4081 tcgctctaca ccttccggct gatcttcgtc gtgttcttcg gcgagccgcg cggtgagatc
     4141 gggcaccgcc cgggctgggc gatgagcgtg ccggtggcca ttctcgcggt cctctcggtg
     4201 gtggccggct tcctcaacgt tccgccgacg ctcggcggga tccacctgtt cgacgacttc
     4261 ctcgcgccgg tgctgcccgc gatggcgcat cacgagggcg gcgggttcac cgaggcggcc
     4321 tcccaggtgc tgacggccct ggcattcggc ctcggtctcg ccgccgcctg ggtgctcttc
     4381 ctccgccgcc gtgacgcggc ggcgcgaatc gcggcttcgc ccccggggca tgcggtgggc
     4441 cggctgtggc gggaaggctg ggggttcgac tggctgtacg aacgggtctt ggtccggccg
     4501 atcaccgcgc tcgcgtcggc cggccgcggc gacttcgttg acggtccctt cgaggccgcc
     4561 gggcgtgcgg cgttcgcgct gcacgtggcg gccagcgcga cacagacggg acgcctgcgg
     4621 tggtacgccg cgggcctggc gggcggctcg ctggccgccg tggcaatcgc gctgttccta
     4681 tgatcctcct gctcctcgtc ctcgtgcccc tcgcgggcgc cgtgctggcc tggctggttg
     4741 agcgtctcgg cgcggcctgg ccgcgggtcg tgtcgctggc cgcgctcggc ggttgtttcg
     4801 gcctcaccgc cgccgcgtgg ctcggccagg cctcgcgcgc ctcgcttcca gcggtggctg
     4861 gtcccggccc ctggctgctg gagttcgacg cggcctggct gccgtcgatc ggcgcgcgct
     4921 tccacctcgc gatggacggc ctgagcctcc cgctggtggg cctcaccttt ttcctgggcc
     4981 tcgccggcgt gatcgcgtcg tggaccgaga tccgcgaacg cgtcggcttc ttccacttca
     5041 acctgctggc cgtgctcgcc ggggtgacgg tcgtcttcct ggcggcggat ctgttcctct
     5101 tctacttcgg ctgggagctg atgctcgtgc cgatgtactt cctgatcgcc atctgggggc
     5161 acgaggggcg tgtccacgcg gcgatcaagt tcttcctctt cacccagatc agcgggctgc
     5221 tgatgctcgt tgccctgctg gcgctctacc tggcgcacgg cgcccagacc ggcctctaca
     5281 ccttcgagta ccgggaactg ctcggcaccg tcctcccgga gcgaaccgag tggtggctga
     5341 tgctcgggtt cttcatcgcg ttcgcggtga agctgccggt cttcccgctc cacacctggc
     5401 tgccggacgc gcacacgcag gcgccgacgg cggggagcgt gatcctggcg gggctgctgc
     5461 tgaagaccgg cggctacggc ctgatccgct tcgtggtgcc gctcttcccg ggagcggccg
     5521 ccgacttcgc cccggtggcc atgacgctcg cggtcgcggg catcctctac ggcgccatcc
     5581 tggctttggg ccagaccgat ctgaagcggc tggtcgccta caccagcgtc agccacctcg
     5641 gcttcgtcct gctcggcgtg ttcgcctgga acacgctggc gctgcagggg gcgctcctcc
     5701 agatggtcgc gcacggcatc agcaccggcg ccttgttcgt cgtcgccggc ttgcttcagg
     5761 agcggatgca cacgcgcgac atcgatcgca tgggcgggct ctggtcggtc gcgccgcgcc
     5821 tcggcggcgt ggcgctcttc ttcgccctgg cctcgctcgg gcttccgggg ctcggcgact
     5881 tcgccgccga gttcctcgtg ctgctcggcg ccttccaggc cagcgccgtc gccgcctcgg
     5941 tggcggcact cggcatggtc gcctcggcgc tgtactcgct ccgcctcgtg cagcgcgcgt
     6001 tccagggcac gaacacgaac gggtggcgca tccccgacct ggtgccgcgc gaggcgctcc
     6061 tgttcgcgcc aatggtggcc gtgctggtct ggctcggcct ctacccgcag ccggtcatcg
     6121 acaccttccg cccagtcgca gagcgcctcc agcaggccac caccctgccc gtcaacgccg
     6181 aaggccgccc ggaagccgtt ctcggggccg aggtgggcca gtgaacgccg aggcgctgcg
     6241 cgcgctgctt ccgctgctcg tcctcgcggc cacccctctg gccgtgatga ttgccatcgc
     6301 cgtccgg
//
LOCUS       DAOPQX010000208         6239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13739_length_6239_cov_4.770375, whole genome shotgun sequence.
ACCESSION   DAOPQX010000208 DAOPQX010000000
VERSION     DAOPQX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13739_length_6239_cov_4.770375"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1435
                     /locus_tag="PKK95_11320"
     CDS             38..1435
                     /locus_tag="PKK95_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18853.1"
                     /translation="MRKTLYVAGREFAATVMTKGFVFGVLITPAIIALVVYFMPRMMN
                     RTPPRIEGRVAIVDATGQVAGRFAEHLTPKAFAEAREEDRRRIAEGTESVRKSVSGTP
                     GAAEAIEQSVDAALGELPQIELEVLPADADPEKAKGLLALPPGERQPDPRQRVALVLV
                     HPDAVEPREQGRYGSYDLFVKAKLDDRLVDHLHDGLRDAIVAARLQAAGMDPSRADGL
                     TDVHRPTSRTVTAAGEAATNEMLNVLLPAAFMGLLLVSVITSGQYLLTSTVEEKSTRV
                     VEVLLSAVSARELMTGKIIGQMAVGLVVLGLYAGLGILALFSFAAMGLLDPVLIGFLF
                     IFFLLAYFSYAAMMAAIGASVSEMREAQSLMTPVMLVIMIPWIMWMPISRDPNSLLAI
                     VLSYIPPVSNFVILLRLTSSSPPPLWQAWLAVAVGAAGVSVLLWFAAKVFRVGLLMFG
                     KPPTLGTLIRWARMS"
     gene            complement(1478..2389)
                     /locus_tag="PKK95_11325"
     CDS             complement(1478..2389)
                     /locus_tag="PKK95_11325"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOC18854.1"
                     /translation="MQYRFTLPLRYVIVVALLASACSSGSTQLVGPAAPKCQATVSGV
                     PTSVPAEGAQVKVSVSAARECGWTASSEASWAVVSPSAGQGAGTLTVRVSPNGAAAPR
                     SGAIVVNDARVGLKQEPAPCRHSLSSTSVTVPHTGGRVSVSVEAPSGCAWTASSAAWI
                     EVATGSGSGAGTAEFVVAPNFGAARSATLTVAGRPWRVDQAGAPAAPPPPDPAPPPAP
                     PPPAPPPPPPAPEEIRLSGRVDDVRGSCPNLEFRLERRSVETDAATSFRSGNCRHVND
                     GRYVRVTGELRGRRIYATIVEIGRDDD"
     gene            complement(2457..4844)
                     /locus_tag="PKK95_11330"
     CDS             complement(2457..4844)
                     /locus_tag="PKK95_11330"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOC18855.1"
                     /translation="MARLLTILAAAAMLFGCEVSTQAPPHPVPPVVAQKARPLPLSDV
                     RLTGGPLRRAQDLDAQYLLSLEPDRMMAFLRKSAGLEPKAEGYNGWDGPGRQLTGHIA
                     GHYLSAVSLMYAATGDARFKERADYLVRELKAVQDKQGDGYIGAQADRDGVDGKIRMM
                     ELSKGIIRSGGFDLNGLWSPWYVLHKIYAGLRDAYRLAGNREALEVEVRFAVWAERLL
                     TTLDAGQTQRMLNTEFGGMNEIFVDLYADTGDKRWLTLADRFHHEAIVGPLARREDIL
                     AGKHGNTLVPKLIGSLARYAYTGRQADLTAAAFFWDQVALHHSFATGGHGRNEYFGEP
                     GKLNDMVEGRTAETCNVYNMIKMARMLFALDPQVRYADFHERALFNHILGSIDPADGA
                     TCYMVPVGRGVAREYQNMHEDFTCCVGSGMESHALHADGLYFEAGDRLWVNLYVPSTA
                     EWRREGVRLVTETDFPDGQHASLTLTMASPKTLTMALRRPWWAGEGFRVLVNGKAEPH
                     LPPPGSYVELRREWASGDRIELTLPKALHLEPLPDNPARVAVMNGPLVLAGDLGPLPE
                     RMTGEDANFALRPSAPVFLAAGQPPDRWLEPIASKPGAFRTRGVGRDRDVDLVPFHQL
                     HRRVYASYWDLYTPAQWKQHEAEVRAAEAKQRQLEAATVGFAQPGQMQAERDANAVIG
                     RSSPVVLAGRHGRRASDYFSVDLPVDPSRPMVLVVTYHPEERANRSTAVLVDGTKVGE
                     QAIPRISPQRQGEFFDVQYPIPNGLVAGKRKVTVRFEATGGNETPAVYGVRMVRR"
     gene            4872..>6239
                     /locus_tag="PKK95_11335"
     CDS             4872..>6239
                     /locus_tag="PKK95_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOC18856.1"
                     /translation="MKTIVRVALWVVLAAALAAGSSGQGPGHTFALGADDFLLDGRPF
                     QIISGELHPARIPAEYWRHRIRMAKAMGCNTVAAYIFWNYHEPAEGRFDFSTGNRDIA
                     RFVRIAQEEGMFVLLRPGPYVCAEWDFGGLPPYLLRDPELKIRCLNPRYMEAAERYMA
                     ALARVVQPLLVTRGGPILMVQLENEYGSYGNDRSYMAHLKDVWVKLGIDVPFFTADGP
                     TPPMLEAGHVEGAAVGLDSGSEEKHWELARSIVPGVPIFSSETYPGWLTHWGEQWARP
                     SLDDLEKEVAFLLGAKKSFNFYVLHGGTNFGFTAGANSGGKGYEPDVTSYDYDAPVSE
                     QGLPTPKYHALRQMIGKAIARPLPPIPEPIPAIDVAEFPMKAHASLWNHLPEPIASVQ
                     PRPFEMYGQNQGLVLYRTRLVGRKSGRLQITDLHDYAMVFVDGALAGTIDRRLGEKAI
                     ELPATA"
BASE COUNT          916 a         2125 c         2223 g          975 t
ORIGIN      
        1 agtcgctgcg cgagcaggac ctggaggagg tggggcggtg agaaagacgc tctacgtggc
       61 cggccgcgag ttcgcggcga ccgtgatgac caaggggttc gtcttcggcg tgctgatcac
      121 ccccgccatc atcgcgctcg tcgtctactt catgccgcgg atgatgaacc ggacgccgcc
      181 gcggatcgag gggcgcgtcg cgatcgtcga cgcgacgggc caggtggccg ggcggttcgc
      241 ggagcacctg acgcccaagg cgttcgccga ggcgcgcgag gaggacaggc gccgcatcgc
      301 cgagggcacc gagagcgtgc gcaagagcgt gtcgggcacg ccgggcgccg ccgaagcgat
      361 cgagcagtcg gtagacgcgg ccctcggcga gctgccgcag atcgagctgg aggtgctgcc
      421 ggctgatgcc gacccggaga aggcaaaggg tctgctcgcg ctgccgccgg gcgagcggca
      481 gccggacccg cgccagcggg tggcgctcgt gctcgtgcat cccgacgccg tggagccgcg
      541 cgaacagggg cgctacggga gttacgacct gttcgtgaag gcgaagctcg acgatcgcct
      601 ggtggaccac ctgcacgacg gcctgcgcga cgcgatcgtc gccgcgcgac tgcaggcggc
      661 cggcatggac ccctcgcgcg ccgatgggct gaccgatgtc cacaggccga cctcgcgcac
      721 ggtgacggcc gcgggcgagg ccgcgaccaa cgagatgctc aacgtcctgc tgccggccgc
      781 gttcatggga ctgctgctcg tgtcggtcat cacgagcggc cagtacctgc tcacttcgac
      841 ggtcgaggag aagtcgaccc gcgtcgtcga ggtgctgctc tcggcggtct cggcccgcga
      901 gctgatgacg ggcaagatca tcgggcagat ggccgtcggc ctcgtcgtgc tcgggctcta
      961 cgcgggactc gggatcctgg ccctgttctc gttcgccgcc atgggcctgc tcgacccggt
     1021 gctcatcggc ttcctcttca tcttcttcct gctcgcctac ttctcctacg cggcgatgat
     1081 ggccgcgatc ggagcgtcgg tgagcgagat gcgcgaggcc cagagcctga tgaccccggt
     1141 gatgctcgtc atcatgatcc cctggatcat gtggatgccg atcagccggg atccgaattc
     1201 gctgctggcg atcgtcctga gctacatccc gccggtcagc aacttcgtca tcctgttgcg
     1261 cctcacctcg agcagtccgc cgccgctgtg gcaggcgtgg ctggcggttg ccgtcggggc
     1321 cgcgggcgtc tccgtgctgc tgtggttcgc cgccaaggtg ttccgcgtcg ggctgctgat
     1381 gttcggcaag ccgcccacgc tcggcacgct aatccggtgg gcgaggatgt catgacgagg
     1441 ccgcgtcgtg ctggaggtgt tgccagcacg acgcgcttca gtcatcatct ctgccgattt
     1501 ccacgatggt cgcgtagatc cgccttccgc gaagctcgcc cgtgacacgg acgtagcggc
     1561 cgtcgttcac gtggcggcag ttgccggaac ggaagctggt tgccgcgtcg gtctcgaccg
     1621 agcgtctctc gagccggaac tccaggttcg ggcaggagcc ccggacgtcg tccacgcggc
     1681 ccgacagcct gatctcctcg ggcgccgggg gaggaggcgg cggcgcgggc ggcggcggcg
     1741 cgggcggtgg ggcggggtcg ggtggtggcg gcgccgccgg tgcgcccgcc tggtcgaccc
     1801 tccacggccg gccggcaacg gtcaaggtgg ccgacctggc ggccccgaag ttcggcgcga
     1861 cgacgaactc ggccgttccg gcgcccgagc cggatcccgt cgccacctcg atccaggccg
     1921 ctgagctcgc cgtccacgcg caaccagaag gtgcttccac cgacacgctg acacggccac
     1981 ccgtgtgggg aaccgtgacc gaagtgctgg agaggctgtg ccggcacggc gccggctctt
     2041 gcttcagccc gacccgagcg tcgttgacga cgatggcgcc cgaacggggc gcggcggcgc
     2101 cattgggtga aacacggacg gtgagggtgc cggccccctg gcccgcggac gggctgacca
     2161 ccgcccagct cgcctcgctg ctggcggtcc agccgcactc gcgggcggcg ctgaccgaca
     2221 ccttgacctg cgccccctct gcggggacgg acgtgggaac gcccgacacg gtggcctggc
     2281 acttcggggc cgccgggccg accagctgcg tcgatccgct gctgcacgcg gatgccagga
     2341 gcgccacaac gatgacgtaa cgaagtggca gggtgaaccg gtactgcaca gctgcacctc
     2401 gactgggagc ggtgcagact ccgtaccggc cccttcctgt gactcgaccg acgggactat
     2461 cgcctgacca tgcgcacccc gtagacggct ggcgtctcgt tgccccccgt ggcctcgaag
     2521 cgaacggtga ccttgcgctt gccggccacc aggccgttcg ggatggggta ttggacgtcg
     2581 aagaactcgc cctggcgctg cgggctgatg cgagggatgg cctgctcgcc caccttcgtc
     2641 ccgtccacca ggacggcggt gctgcggttg gcgcgttcct cggggtggta ggtcacgacc
     2701 agcaccatcg ggcgagacgg gtcgaccggc aggtcgacgg agaagtagtc gctcgcgcgg
     2761 cggccatggc gtccggcgag cacgaccggt gaagagcggc cgatcacggc gttcgcgtcg
     2821 cgttcggcct gcatctgccc gggctgcgcg aagccgacgg tggccgcctc gagctgacgc
     2881 tgcttcgcct cggcggcccg gacctctgcc tcgtgctgct tccactgggc cggggtgtag
     2941 aggtcccagt aggaggcgta gacccgccgg tggagctggt ggaacgggac gagatcgacg
     3001 tcgcggtcgc ggccgactcc cctggtgcgg aaggcgcccg gtttcgaggc gatcggctcg
     3061 agccaccgat cgggcggctg gccggccgcg aggaagaccg gggccgacgg ccgcagggcg
     3121 aagttcgcgt cctcgccagt catgcgctcg ggcaggggac cgaggtcgcc ggccagcacg
     3181 agcgggccgt tcatcacggc gacgcgcgcg gggttgtcgg gcagcggctc gaggtgaagc
     3241 gccttcggca gcgtcagctc gatccgatcg cccgatgccc actcgcgccg cagctccacg
     3301 taggagccgg gcggcggcag gtgtggctcg gccttcccat tcaccagcac gcgaaagcct
     3361 tcgcccgccc accacgggcg ccgcagcgcc atcgtcagcg tcttcggcga agccatcgtc
     3421 aacgtgagcg acgcgtgctg cccgtctggg aaatcggtct ccgtgaccag gcgcacgccc
     3481 tcgcggcgcc actcggcggt ggacggcacg tacaggttca cccacagccg gtcgcctgcc
     3541 tcgaaataca ggccatcggc gtgcagcgcg tggctctcca tgcctgagcc gacacagcag
     3601 gtgaagtcct cgtgcatgtt ctggtattcg cgcgccacgc cgcgcccgac cggcaccatg
     3661 tagcaggtcg ccccatcggc cgggtcgatc gatccgagga tgtggttgaa gagggcgcgt
     3721 tcgtggaagt cggcgtagcg gacctggggg tcgagggcga acagcatccg cgccatcttg
     3781 atcatgttgt acacgttgca cgtctcggcg gtccgcccct ccaccatgtc gttcagcttc
     3841 ccgggctcgc cgaagtactc gttgcggccg tggccgccgg tggcgaagct gtggtggagc
     3901 gccacctggt cccagaagaa ggcggcggcg gtgaggtcgg cctgcctgcc cgtgtaggcg
     3961 tagcgcgcga gcgagccgat cagcttcggc acgagcgtgt tgccgtgctt cccggccagg
     4021 atgtcctcgc gccgcgcgag cgggccgacg atggcctcgt ggtggaagcg gtcggcgagc
     4081 gtcagccacc gcttgtcgcc ggtgtcggcg tacaggtcca cgaagatctc gttcatcccg
     4141 ccgaactcgg tgttcagcat ccgctgcgtc tggccggcgt cgagcgtggt cagcagccgc
     4201 tcggcccaca cggcgaacct cacctcgacc tcgagcgcct cgcggttgcc ggcgaggcgg
     4261 taggcgtcgc gcagccccgc gtagatcttg tgcaggacgt accagggcga ccagaggcca
     4321 ttcaggtcga agccgccgga gcggatgatc cccttggaaa gctccatcat ccggatcttg
     4381 ccgtccactc cgtcgcggtc ggcctgcgcc ccgatgtagc cgtcgccctg cttgtcctga
     4441 acggccttca gttcgcgcac gaggtagtcg gcgcgctcct tgaagcgggc gtcgcccgtc
     4501 gccgcataca tcaggctcac cgcggagagg tagtgccctg cgatgtgacc ggtcagctgg
     4561 cgtcccgggc catcccaccc gttgtagccc tcggccttcg gctcgaggcc ggcgctcttg
     4621 cggaggaagg ccatcatgcg gtcgggctcg agcgacagca ggtactgcgc gtcgaggtcc
     4681 tgcgcacgcc tgagggggcc gccggtgagg cggacgtcgg agagcgggag gggccgcgcc
     4741 ttctgagcga caacgggcgg gaccgggtgc ggcggggcct gcgtggacac ctcacatccg
     4801 aagagcatcg cggcggcggc caggatcgtc aggagtcgtg ccatggaccc aaatggtagt
     4861 agattccgga tatgaagacg atcgttcgcg ttgcgctgtg ggttgtcctc gcggccgcac
     4921 tggcagccgg ttcctcgggg caggggcctg ggcacacgtt cgcgcttggt gccgacgact
     4981 tcctcctcga cggccggccg ttccagatca tctcgggcga gctgcacccg gctcgaatcc
     5041 ccgcggaata ctggcgccac cgcatccgca tggcgaaggc gatggggtgc aacaccgtcg
     5101 cggcctacat cttctggaac taccacgagc cggccgaggg ccgcttcgac ttttccaccg
     5161 gcaaccgcga catcgcccgc ttcgtccgga tcgcccagga agaagggatg ttcgtcctcc
     5221 tgcggcccgg cccctacgtc tgcgccgaat gggatttcgg cggcctgccg ccatacctgc
     5281 tgcgcgaccc cgagctgaag atccgctgcc tgaacccgcg ctacatggag gcggccgaac
     5341 gatacatggc cgcgctggcg agggtggttc agccgctgct ggtgacccgc ggcggcccca
     5401 tcctgatggt gcagctcgag aacgagtacg ggagctacgg gaacgaccgg tcctacatgg
     5461 cgcatctcaa ggacgtctgg gtgaagctgg ggatcgacgt gcccttcttc accgcggacg
     5521 ggccgacccc gcccatgctc gaggccgggc acgtcgaggg cgcggccgtt ggcctcgact
     5581 cgggctcaga ggagaagcac tgggagctgg cgcggtcgat cgtcccgggc gttcccatct
     5641 tctcgtccga aacctaccca ggctggctga cgcactgggg cgaacagtgg gcgcggccct
     5701 cgctcgacga cctcgagaaa gaggtggctt tcctgctcgg cgcgaagaag tcgttcaact
     5761 tctacgtgct gcacggcggc accaacttcg gcttcaccgc cggcgcgaac tcgggcggca
     5821 aggggtacga gcccgacgtc accagctacg actacgacgc gccggtgagc gaacaggggc
     5881 tgccgacgcc gaagtaccac gcgcttcgcc agatgatcgg aaaggcgatc gcgaggccgc
     5941 tgccgccgat ccccgagccg atcccggcga tcgacgtcgc cgagttcccg atgaaggcac
     6001 acgcctctct ctggaaccac ctgccggaac ccatcgcctc ggtgcagccc aggccgttcg
     6061 agatgtacgg ccagaaccag gggctcgtgc tgtaccggac caggctcgtc ggcaggaaga
     6121 gcgggcggct gcagattacc gacctccacg actacgcgat ggtgttcgtg gacggggcgc
     6181 tcgccggcac gatcgaccgg cggcttggcg agaaggcgat cgagctgccg gccaccgcc
//
LOCUS       DAOPQX010000209         6179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13919_length_6179_cov_4.590464, whole genome shotgun sequence.
ACCESSION   DAOPQX010000209 DAOPQX010000000
VERSION     DAOPQX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13919_length_6179_cov_4.590464"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..985)
                     /locus_tag="PKK95_11340"
     CDS             complement(86..985)
                     /locus_tag="PKK95_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOC18857.1"
                     /translation="MTRREVMTLGGAAAVAAARGIRTQAAGPDRALGIQLYTVAADFE
                     KDARATLRALSKIGYRFVESAGFDKIPAREFRKALDEAGLSCPSCHLPFSSADPRPLF
                     EDAQTVGARYATASVLMPPAAAVATPADLFKALATLTLDDFKKIAVLANEIGAKARDA
                     GLQFVYHNHNFEFRDHGGKTGWHVLLAETDANLVKLELDCGWATAAGQDVLALLRQHA
                     GRFPMLHVKDFVLKGKPSHALFGPDRPAGTELGRGQIDYKAIFAAARAAGVEYYFSEQ
                     EPPIVNMTPLEAAKVNFEYMRAL"
     gene            complement(1116..2516)
                     /locus_tag="PKK95_11345"
     CDS             complement(1116..2516)
                     /locus_tag="PKK95_11345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOC18858.1"
                     /translation="MRPPERFLAAIAALTLAGGCSLAPKYARPAAPVPPELPAPVVAG
                     PAGEVPPIAPWREFVADERLRAVVEMTLANNRDLRTAALNAERVGAFYRIQRAELYPS
                     IGASAGVQVSHVPDSVAGEGEGYTSHPYSLSVGFTSWELDLFGRIRSLKEQALNEYLA
                     AQQVAAATQTSLIAAAASTYLALAADAEEKRLAEATLAAQRQTLEMIQKSREVGVASD
                     LDVRQVQTQVESARALVARYGGLVETDRHALELLAGRPIPPDLLPGSLAEVAPLQPVA
                     AGVSSEVLLRRPDVIAAEYQLKGLNASIGAARAALFPRISLTAAIGLVSPDLSNLFKG
                     SSGAWSFAPQAAAPIFNAGALKANVEATRLARDAAVAQYEKAIQSAFREASDALVLRT
                     SLVEQRDAEAALVKALEETYRLSEARYKAGIDGYLGVLVAQRALFSAQQALVGVRLAE
                     QQNLVNLYKVLGGGVS"
     gene            complement(2518..5694)
                     /locus_tag="PKK95_11350"
     CDS             complement(2518..5694)
                     /locus_tag="PKK95_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC18859.1"
                     /translation="MSHFFLKRPVFAWVIAIGLMLGGGLAIYNLPISQYPPIAPPSIA
                     IRTSYPGASAETVENTIIQMIETVMIGLDRMLYMRSTADSAGSGSVELTFAPGTDPDI
                     AWSKVQNKLQLALPSLPDVVKSRGVTVAKSTRNYLLIIGLTTEDGSLSQEDLNDYSVS
                     QLQPAIGRVEGVGEVDVFGSGYSMRIWLDPDKLAQYGMTIDDVVAAVRTYNVEVSAGQ
                     LGGAPALPGQRLNASILVQSLLVTPQEFAAVPLRMNADGSVVRVSDVGRTELGNEVPD
                     IQGRFNGHPMSGMAVRMEAGANALDTANRIKAKLEELSRFFPPGMKVVYPYDTTPFIE
                     VAIWEVVKTLFEAIVLVFLVMYLFLGNLRATLIPTIAVPVVILGTFGVLAVLGFSINM
                     LTMFAMVLAIGLLVDDAIVVVENVERVMTEEGLPPWEATRTSMGEISSALVGIGLVLS
                     AVFAPMIFFPGSTGVIYRQFSATIIASMLLSVAVALILTPVLCAQLLKPVEKGHEAAE
                     TGSRLLRPFFLGFDRAFYRARDGYAWFVARILGRKKRYLAAFAAVVAVMAVLYARMPT
                     AYLPDEDQGTVMTMVQLPVGSTLEQTRSTMDVIQNYYRSEHPDAVEAVMAMSGIGFAG
                     RGQNQGMMYVKLKDWDLRDRADLRAPAVAGRAMAALSKLRATIYPIIPPPMPELGTAT
                     GFDLMLQDRGGLGHQKLIEAQNQLLMMAAQDPRLARVRPLGMPDAPMYKVDIDWHKAG
                     ALGVPVNQVQSYLAGALGGAYIGNFVQGGRVKRVYAQAEARFRMLPGDLSHLWMKNRS
                     GELVPLSAVASGRWVHGSSRLERYNSFPAVNIQGEAAPGHSTGEAMRAVEEIVQKLPA
                     GIGHEWTGVSYQQRTAESQTGLLYAFSVLAIFLVLAALYESWTVPITVLFALPLGVIG
                     GVIASSLRGMPNDVYFQIGLLTVLGLTTKNAILIVQFAKARIEEEGMGLIEATVEAAK
                     LRLRPIVMTSLAFGFGVLPLALARGAGAGAMKAIGTSVLGGMVTGTFLAIFFIPLFFV
                     IIVKVFGSKRRRVEAAHLEPALDGVEGK"
     gene            complement(5704..>6179)
                     /locus_tag="PKK95_11355"
     CDS             complement(5704..>6179)
                     /locus_tag="PKK95_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC18860.1"
                     /translation="KVKLLLEDGTPYPEEGTLQFRDVSVDPTTGSVILRMVFPNPRRT
                     LLPGMYVRAIVEEGVNEQAILAPQQGVTRNPKGEAIALVVNASGKVEQRIVEVDRAVG
                     AEWVVTGGLAPGDRVVVEGGDQVRAGAEVRAVEVALAPGAAPASVVPASAVPAGK"
BASE COUNT          938 a         2212 c         2103 g          926 t
ORIGIN      
        1 gagggggggc gggccgttgg ctgaagctgg tgcgccgcgg cagcaccttc acggcctacg
       61 cgtcaccgcc ccgcgtcgta tgcccctaca gggcccgcat gtactcgaag ttgaccttcg
      121 cggcctcgag cggcgtcatg ttgacgatgg gcggctcctg ctccgagaag tagtattcga
      181 cgcccgcggc ccgggcggcc gcgaagatcg ccttgtagtc gatctgcccg cggccaagct
      241 cggtcccggc gggacggtcg ggcccgaaca gggcgtgcga cggctttccc ttcagcacga
      301 agtccttgac gtgcagcatc gggaagcggc ccgcgtgctg gcgcaacagg gcgaggacgt
      361 cctggcccgc ggcggtcgcc cagccgcagt cgagctccag cttgacgagg ttggcgtcgg
      421 tttccgcgag cagcacgtgc cagccggtct tcccgccgtg gtcccggaac tcgaagttgt
      481 ggttgtggta gacgaactgg aggcccgcgt cacgcgcctt ggctccgatc tcgttcgcca
      541 ggacggcaat cttcttgaag tcgtcgagtg tcagcgtggc cagcgccttg aacaggtcag
      601 cgggcgtggc cactgccgcc gcgggaggca tcagcaccga ggccgtggcg taccgagcgc
      661 cgacggtctg cgcatcctcg aacagcgggc ggggatcggc gctcgagaag ggcagatggc
      721 agctcgggca cgagaggccg gcttcgtcca gtgccttgcg gaactcccgt gccgggatct
      781 tgtcgaagcc ggccgattcg acgaacctgt agccgatctt cgacagcgcc cgcagcgtcg
      841 cccgcgcgtc cttctcgaaa tcggcggcga cggtgtacag ctggatgccc agcgcccggt
      901 cagggcctgc cgcctgcgtt ctgatcccgc gcgccgccgc gacggccgcc gctcccccca
      961 gggtcatcac ctcgcgtctc gtcatcactg gcatgagcgc ctccgtgtgg cgctactgta
     1021 tcaccagccc ggcttgatgc ggtggcgcga cgccggccgg gccttgaccc tgggcatcag
     1081 ctggccgttc ggcacctgac cgttcgccac ccgacctacg acacgccgcc cccgagcacc
     1141 ttgtacaggt tcaccaggtt ctgctgctcg gctaggcgga cccccacgag cgcctgctgc
     1201 gcggaaaaca gcgcccgctg cgcgacgagg acgcccaggt agccgtcgat gcccgccttg
     1261 tagcgcgcct ccgacagcct gtaggtttcc tcgagcgcct tgacgagggc cgcctcggcg
     1321 tcgcgctgct cgacgagcga cgtgcgcagc accagcgcgt cgctcgcctc gcggaacgcg
     1381 gactggatcg ccttctcgta ctgggcgacc gccgcgtcgc gcgccaggcg cgtcgcctct
     1441 acgttcgcct tcagcgcccc cgcgttgaag atcggtgctg ctgcctgcgg ggcgaacgac
     1501 caggccccgg acgacccctt gaacaggttg gagaggtcgg ggctcaccag gccgatggcc
     1561 gccgtcagcg agattcgcgg gaacagcgcg gcccgcgccg cgccgatcga ggcattcaag
     1621 cctttcagct ggtactcggc ggcgatgaca tcggggcgcc ggagcaggac ctctgacgag
     1681 acccctgcgg caaccggctg caagggggca acctcggcaa ggctgcccgg cagcagatcg
     1741 ggcgggattg ggcgtcccgc cagcagctcg agcgcgtggc gatcggtctc gaccaggccg
     1801 ccgtagcgcg cgacgagcgc gcgggcgctc tcgacctgcg tctgcacctg gcggacgtcg
     1861 aggtcggagg ccacgcccac ctcccggctc ttctggatca tctccagcgt ctgccgctgc
     1921 gccgcgaggg tcgcctcggc gagtcgcttc tcctcggcat cggccgccag cgccaggtag
     1981 gtgctcgcag cggctgcgat cagcgaggtc tgcgtggccg cggccacctg ctgcgcggcc
     2041 aggtactcgt tcagcgcctg ctccttgagg ctgcggatgc gcccgaacag gtccagctcc
     2101 cacgaggtga agccgacgct cagcgagtag gggtgcgacg tgtagccctc gccctctccc
     2161 gccacgctgt cgggcacgtg agagacctgc acgcccgcgg acgcaccgat cgacgggtag
     2221 agctcggctc gctggatccg gtagaaggcg cccacgcgct cggcgttcag cgccgccgtc
     2281 cggaggtcgc ggttgttcgc cagggtcatc tcgaccaccg ctcgcagccg ctcgtcggcg
     2341 acgaactcgc gccacggcgc gatcggcggc acttccccgg cgggaccggc cacgacgggg
     2401 gccggcagct cggggggaac gggcgccgcg gggcgggcgt acttcggggc cagcgagcag
     2461 ccgcccgcca gcgtcagcgc ggcaatggcc gcgaggaacc gttctggtgg ccgcatgtca
     2521 cttcccctcc acgccgtcga gggccggctc caggtgcgcc gcttccaccc gcctgcgctt
     2581 gcttccgaat accttgacga tgatcacgaa gaagagcggg atgaagaaga tcgccaggaa
     2641 agtaccggtc accatcccgc ccagcacgct ggtgccgatt gccttcatcg cgccggcgcc
     2701 cgcgccgcgc gcgagcgcga gcggcagcac gccgaatcca aacgcgagcg aggtcatcac
     2761 gatcgggcgc agccggagct tggcggcctc gacggtcgcc tcgatcaggc ccatgccctc
     2821 ctcctctatc cgcgccttgg cgaactgcac gatcaggatg gcgttcttgg tcgtgaggcc
     2881 cagcacggtc agcaagccga tctggaagta cacgtcgttc ggcatcccgc gcagcgacga
     2941 ggcgatgacc ccgccgatga cgccgagcgg cagcgcgaag aggacggtga tcggcaccgt
     3001 ccagctctcg tacagggcgg ccagcacgag gaagatcgcc agcacggaga acgcgtagag
     3061 cagcccggtc tgtgactcgg ccgtccgctg ctggtacgac acgccggtcc actcgtgccc
     3121 gatgccggcc ggcagcttct gcacgatctc ctcgaccgcc cgcatcgcct cgccggtgga
     3181 gtgccccggc gcggcctcgc cctggatgtt caccgccggg aagctgttgt accgctcgag
     3241 cctcgacgac ccgtgcaccc agcgccccga ggcgacggcc gagagcggca ccagttcgcc
     3301 gctccggttc ttcatccaca gatgcgagag gtcgcccggc agcatccgga acctggcctc
     3361 ggcctgcgcg tagacacgct tcacccgccc gccctgcacg aagttgccga tgtacgcgcc
     3421 gcccaacgct cccgccaggt agctctgcac ctggttgacg gggacgccga gcgcgcccgc
     3481 cttgtgccag tcgatgtcca ccttgtacat cggggcgtcg ggcatcccga ggggcctcac
     3541 ccgcgccagc cgcgggtctt gcgccgccat catcagcagc tggttctgcg cctcgatcag
     3601 cttctggtgg ccgaggccgc cgcggtcctg gagcatcagg tcgaagccgg tggcggtgcc
     3661 cagctcgggc atgggcgggg gaatgatcgg gtagatggtg gcgcgaagct tcgagagggc
     3721 ggccatcgcc cgccccgcca cggccggcgc gcgcaggtcg gcgcggtcgc gcaggtccca
     3781 gtccttcagc ttcacgtaca tcattccctg gttctgtccg cgacccgcga acccgatgcc
     3841 gctcatcgcc atgacggcct ccacggcgtc ggggtgctcg gaccggtagt agttctggat
     3901 cacgtccatc gtcgaccgcg tctgctccag cgtggagccg accggcagct gcaccatcgt
     3961 catcacggtg ccctggtcct cgtcggggag gtaggccgtg ggcatccgcg cgtagagcac
     4021 cgccatcacc gccaccacgg cggcgaaggc cgcgagatag cgcttcttcc gcccgaggat
     4081 gcgggccacg aaccaggcat agccgtcgcg ggcgcggtag aacgcacggt cgaagccgag
     4141 gaagaacggg cgcagcaggc gggagccggt ctcggccgcc tcgtgcccct tctcgaccgg
     4201 cttcagcagc tgggcgcaca gcaccggggt gaggatcagg gccaccgcca ccgagagcag
     4261 catcgaggcg atgatggtcg ccgagaactg ccggtagatg acgcccgtgg accccgggaa
     4321 gaagatcatc ggcgcgaaga ccgctgagag caccaggccg atgccgacca gcgcgctgct
     4381 gatctccccc atcgacgtgc gcgtcgcttc ccacggcggc aggccttcct cggtcatcac
     4441 gcgctcgacg ttctcgacca cgacgatcgc gtcgtccacc agcagcccga tcgccagcac
     4501 catcgcgaac atcgtcagca tgttgatgga gaagccgagc acggcgagga cgccgaaggt
     4561 gccgaggatc accacgggca ccgcgatggt cgggatcagc gtggcccgca ggttgccgag
     4621 gaacaggtac atcacgagga agaccaggac gatcgcctcg aacagcgtct tgacgacctc
     4681 ccaaatcgcc acctcgatga agggcgtcgt gtcgtacggg tagacgacct tcatgcccgg
     4741 cgggaagaag cgcgacagct cctccagctt tgccttgatg cggttggcgg tgtcgagggc
     4801 gttcgcgccg gcctccatgc ggaccgccat gccgctcatc gggtggccgt tgaagcgccc
     4861 ctggatgtcg ggcacctcgt tgccgagctc ggtccgcccg acgtccgaga cgcgcaccac
     4921 cgagccgtcg gcgttcatgc gcagcggaac ggcggcgaac tcctgcggcg tcacgagcag
     4981 cgactggacg aggatcgacg cgttcaggcg ctggcccggc agcgcggggg cgccccccag
     5041 ctggcctgcc gagacctcca cgttgtaggt ccgcacggcc gccacgacgt cgtcaatggt
     5101 catcccgtac tgcgcgagct tgtcggggtc gagccagatg cgcatcgagt agccgctgcc
     5161 gaacacgtcg acctcgccca ccccctcgac ccggccgatg gccggctgca gctgcgacac
     5221 cgagtagtcg ttcaggtcct cctgcgacag gctgccgtcc tcggtcgtca gcccgatgat
     5281 gaggaggtag ttgcgggtcg acttggccac ggtgacgccg cgcgacttca ccacgtcggg
     5341 cagcgacggc agggcgagct ggagcttgtt ctggaccttc gaccaggcga tgtcggggtc
     5401 ggtgccgggc gcgaaggtca gctccaccga gcccgagccc gccgagtcgg ccgtcgaccg
     5461 catgtagagc atccggtcga gcccgatcat gaccgtctcg atcatctgga tgatcgtgtt
     5521 ctcgaccgtc tcggccgagg cccccgggta ggacgtgcga atcgcgatcg acggcggagc
     5581 gatcggcggg tactgcgaga tcgggaggtt gtagatcgcc aggccgccgc caagcatcag
     5641 gccgatggcg atcacccagg cgaagacggg ccgcttgagg aagaagtgcg acattgccgc
     5701 ctcctacttc cccgccggga cggcactggc cgggacgacg cttgcgggcg cggcgccggg
     5761 cgcaagcgcc acttcgacgg cccgcacctc ggcgcccgcc ctgacctggt ctccaccctc
     5821 gacgaccacg cggtcgccgg gagcgaggcc gccggtcacg acccactcgg cgccgaccgc
     5881 gcggtccacc tcgacgatcc gctgctccac cttgcccgag gcgttcacca ccagcgcgat
     5941 cgcctctccc ttggggttgc gggtcacgcc ctgctgcggc gcgaggatgg cctgctcgtt
     6001 cacaccctcc tcgacgatcg cccggacgta catgccgggc agcagcgtgc gccgcgggtt
     6061 cgggaagacc atgcgcagga tcaccgagcc ggtcgtcgga tccaccgaca cgtcgcggaa
     6121 ctgcagcgtg ccttcctccg ggtacggcgt gccgtcctcg agcagcagct tcacctttc
//
LOCUS       DAOPQX010000210         6154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_13994_length_6154_cov_4.303000, whole genome shotgun sequence.
ACCESSION   DAOPQX010000210 DAOPQX010000000
VERSION     DAOPQX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13994_length_6154_cov_4.303000"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKK95_11360"
     CDS             complement(<1..545)
                     /locus_tag="PKK95_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOC18861.1"
                     /translation="MSASHDHGPHRAGNASRRAGARSLSIVLFMVVGYMVAEVVGGLL
                     TNSLALLADAGHMLSDAAALGLALFALWFAGRPAPSNRTYGYYRIEILAALANGATLV
                     AVAAFILIEAFQRISSPPPVDGGPMMAIAAGGLLVNLLALRLLSEGRGESLNVRGAWL
                     HVLTDALGSVQAIVAGALIVW"
     gene            719..1987
                     /locus_tag="PKK95_11365"
     CDS             719..1987
                     /locus_tag="PKK95_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18862.1"
                     /translation="MADFYQTGVVTTLHRLGSANLERIEAELTDIARTERIALVLPAL
                     YAEFTRPAMPRIIKELRHVEYLHEIVVSLGRATAEEFEAARAMLSVLPQKVSIIWNDG
                     PGVRSLYERLAAHNLPVGEDGKGRSCWMAYGYLLATDDCDVIALHDCDITTYNRELLA
                     RLCYPVANPNLGFEFSKGFYSRVGRKMHGRVTRLFVTPLIRTLQRLVGELPLLVYLDS
                     FRYALAGEFAMRTDLARVTRIPSDWGLEVGMLAEVYRNCALKRICQTEICENYDHKHQ
                     EMKAGDSTRGLVKMCVDIAQNLLRTLAAEGAVFTEGLFRTLSVQYIRMAEDTINRYHA
                     DAAINGLSFDRNEEESTVVAFAEGLRLACQRYLEDPLGAPLIPNWNRVVSALPDFYDQ
                     LLEAVAADNAASRVPVAARKGTAMAPMEAA"
     gene            1987..2856
                     /locus_tag="PKK95_11370"
     CDS             1987..2856
                     /locus_tag="PKK95_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOC18863.1"
                     /translation="MGPRPACVVFTDVDGCLVDHTTYSFEEAAAALARLRARGVPLVL
                     CSSKTRAEVERLQQELGTTDPFVSENGGGLFVPEGYFPFDLPGARPMAGYQLIELGRP
                     YTEVVEALRAAARKSGVRVAGFNDMTVAEVAAETGLSLPAARLAKLREYDEPFRIVEG
                     DPAQRARMFRALSASGLRATEGGRYHHVGGPTDKGVAVGLLLRFYRWAHGQLVSVGLG
                     DGPNDVPFLRLVDLAFVVANPASGATERVSRQVPSARVTTAPGPAGWHEAMAGILGNG
                     DAARVRERAGLDA"
     gene            2860..4125
                     /locus_tag="PKK95_11375"
     CDS             2860..4125
                     /locus_tag="PKK95_11375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC18864.1"
                     /translation="MAKAEVLPPRAVSAVEAIDRADIVVGIPSFNNARTIGHVVEATH
                     AGLVKYFPQSRAIIVNSDGGSTDGTQEIVTGLQVQSSDVLLISHPIFPVHRLTIPYHG
                     LPGKGSAFRCIFRVAEKLGAKACAVVDSDLRSINPEWVQLLIAPLLQQGYDYVSPYYF
                     RHKYDGTITNSIVYPLTRALYGQRVRQPIGGDFGFSGRLASHFLAQPVWDTDVARYGI
                     DIWMTTTAICGGFRVCQSFLGSKLHDAKDPGADLSAMLVQVLGTVFSLMETHEDVWRR
                     VSGSEETPLMGFRFAVGVEPIAVNTQRMLDHFRNGVSNLRDVWSLVLGPADLWCLEEA
                     ARARPEAFRLDDGLWTRIVYDFAAAYHHRRIDRQHLIRSFLPLYMGRVASFVREVAES
                     DAPAVEERLEQLCRVFEENKAHLAKRWSS"
     gene            4138..4839
                     /locus_tag="PKK95_11380"
     CDS             4138..4839
                     /locus_tag="PKK95_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18865.1"
                     /translation="MLEKLQLVLQESWESAAIELTRFLPHLLGAAVLIVVGAGLGWLA
                     GRITRWLLWRARADLGAERLGIMGPARRLGIESPSAFIARVVRWGITLAAFVPALYSL
                     DARLAADLVRRFLLYLPHLVVAVGLLWAGFLVSRFVGRSVLIAAVNTGFGSARLLAAL
                     ARWGIVLVVLAVALEHLGIGRATVLTAFAILFGGITLALAIAAGFGSQDVVRQWWAGR
                     LQLPEKRDEEPFRHW"
     gene            complement(4826..>6154)
                     /locus_tag="PKK95_11385"
     CDS             complement(4826..>6154)
                     /locus_tag="PKK95_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOC18866.1"
                     /translation="ARGRAVTRRLAALGIVLLLALALARVTAPAAGPRTTALALGIAL
                     LAASLAGDLLERARLPRLTGYLLFGVVCGPYLLNIITRPMARDLQLIDNLAVALIALV
                     AGLEMNLGRLRRSLKAILALAGTTIGLMYVALLAVLWAAWPWLPIDPAASGLLRFAQA
                     AVLTTIVVSFSPAATMAVIAESRARGPLTEITLAVVIMAEIVLIVGFTLVVQLLRHAA
                     GAGPGADVGVSALLAWELIGSLALGSLLGALFALYLHHVGREVSIVLLFLCVVLTQLS
                     RALHTEMLLTALAAGFVVENLVPPEGDALKDAVERGSPPVLVVFFAAAGASLQIDALA
                     EVGVLAVALAGLRMLLLRSGVMLGTRLPSVPDPPGRLVWMGLVSQAGVVLGLAIVVAR
                     QFPGWGLRLQTVVVALVAINQFLGPAVFRLALARAGEVGGADRQVPSTSA"
BASE COUNT          946 a         2216 c         2137 g          855 t
ORIGIN      
        1 aaccagacga tgagcgcgcc ggctacaatc gcctggacgc tgccgagagc gtcggtcaga
       61 acgtgcagcc acgcgccgcg cacgttgagg ctctcgcccc gcccctcgct cagcagccgc
      121 agcgcgagga ggttgacgag caggccgcca gcggcgatgg ccatcatcgg ccccccgtcg
      181 acggggggcg ggctcgagat gcgctggaaa gcctcgatca ggatgaacgc ggcaaccgcg
      241 accagcgtgg cgccgttggc cagcgccgcc aggatctcga tccggtagta gccgtaggtg
      301 cggttggacg gggcgggccg gcccgcgaac cagagcgcga agagcgccag gccgagggcc
      361 gccgcgtccg acagcatgtg ccccgcgtcg gcgagcaggg ccagcgagtt ggtgagcagg
      421 ccgccgacga cctcggccac catgtagccc accaccatga acaggacgat cgacagcgag
      481 cgggcaccgg cgcggcggct ggcgttcccg gcccgatggg ggccgtggtc gtggctggcg
      541 gacatctttc ttcattctaa cggccccggc gggctgccga agagcgccga gcggcccttt
      601 tccctccctt ccccgcccca gaatcccgtc cggacgggat ggcctctccg gcggccgccc
      661 actagactcg gcgcatcggt tttcaagcct gagtggccca gttgaaagga gtgtcgggat
      721 ggccgatttc tatcagaccg gtgtcgtgac cacgctccac cgcctgggat cggccaacct
      781 ggaacgaatc gaggccgaac tcaccgacat cgccaggacc gagcggatcg cgctcgtcct
      841 gccggcgctg tacgcggagt tcacgcgccc cgcgatgccg cggatcatca aggaactgcg
      901 gcacgtcgag tacctgcacg agatcgtggt gtcgctgggg cgcgcgaccg ccgaggagtt
      961 cgaggcggcc cgagccatgc tctcggtcct gccccagaag gtgagcatca tctggaacga
     1021 cggccccggg gtccgttcgc tgtacgaacg cctggccgcc cacaatctcc cggtcgggga
     1081 ggacggcaag ggccggtcgt gctggatggc gtacggctac ctgctcgcga ccgacgactg
     1141 cgacgtgatc gcgctgcacg actgcgacat caccacctac aaccgcgagc tgctcgcgcg
     1201 cctctgctac cccgtggcca acccgaacct cggcttcgag ttctcgaagg ggttctacag
     1261 ccgggtgggc cggaagatgc acgggagggt gacgcgcctg ttcgtcaccc cgctgatccg
     1321 cacgctgcag cgcctggtcg gcgagctgcc gctgctcgtc tacctcgact cgttccgcta
     1381 cgcgctggcc ggggagttcg ccatgcggac cgacctcgcc cgtgtcacgc ggatcccctc
     1441 cgactggggg ctcgaggtcg gcatgctggc cgaggtgtac cgcaactgcg cgttgaagcg
     1501 gatctgccag acggaaatct gcgagaacta cgatcacaag caccaggaga tgaaggcggg
     1561 cgactcgacc cggggcctgg tgaagatgtg cgtggacatc gcgcagaacc tgctccgcac
     1621 gctggcggcc gagggggcgg tcttcaccga ggggctgttc cggacgctct cggtgcagta
     1681 catccggatg gcagaggaca ccatcaaccg ctatcacgcc gacgcggcca tcaatggcct
     1741 ctccttcgac cgcaacgagg aggagagcac cgtggtcgcc ttcgcggagg gcctccgcct
     1801 cgcgtgccag cgctacctcg aggacccgct gggagcgccg ctcatcccca actggaaccg
     1861 ggtggtgtcg gcgctgcccg atttctacga ccagctgctc gaggcggtcg ccgccgacaa
     1921 tgccgcctcc cgggttccgg tcgccgcccg caagggcacc gcgatggccc caatggaggc
     1981 cgcatagtgg gcccacgacc agcgtgcgtg gtgttcaccg acgtggacgg gtgcctcgtc
     2041 gatcacacca cctactcgtt cgaggaagcg gcggcggcac tcgcccgcct gcgcgcgcgc
     2101 ggcgtgccgc tggtcctctg ctcgagcaag acccgcgcgg aggtcgagcg cctgcagcag
     2161 gagctcggca cgaccgaccc gttcgtgagc gagaacggcg gcggcctgtt cgtgcccgag
     2221 ggctatttcc cgttcgacct gcccggtgcg cggccaatgg ccggctacca gctgatcgag
     2281 ctggggcggc cgtacaccga ggtggtcgag gcgctccgcg ccgccgcccg aaagagcggc
     2341 gtccgggtgg cggggttcaa cgacatgacg gtcgcggagg tggccgcgga gacgggcctt
     2401 tcgcttccag ccgcccgcct ggcgaagctc cgcgagtacg acgagccgtt ccggattgtc
     2461 gagggagacc cggcgcagcg cgcccggatg ttccgcgccc tctcggccag cggcctccgg
     2521 gccaccgaag gcggccgtta ccatcacgtg ggcggcccga ccgacaaggg ggtggccgtc
     2581 ggcctcctgc tgcgcttcta ccgctgggcc cacgggcagc tcgtgtcggt cgggctcggg
     2641 gacggcccga acgacgtgcc cttcctgcgc ctggtggacc tcgcgttcgt ggtggccaac
     2701 ccggccagcg gcgcgaccga gcgggtgtcg cgccaggtgc cgtccgcccg ggtcaccacc
     2761 gcgcccggcc cggccggctg gcacgaggcg atggcgggca tcctcgggaa cggcgacgcg
     2821 gccagggtgc gcgagcgcgc cgggctcgat gcgtaaccca tggcaaaggc tgaagtcctc
     2881 ccgccgcgcg cggtcagtgc cgtcgaggcc atcgaccgcg ccgacatcgt cgtcggcatc
     2941 ccgagcttca acaacgcgcg caccatcggc cacgtggtcg aggcgacgca cgccgggctc
     3001 gtgaagtact tcccgcagtc gcgggcgatc atcgtcaact ccgacggcgg ctcgaccgac
     3061 ggcacgcagg agatcgtgac ggggctgcag gtgcagtcga gcgacgtgct cctcatctcg
     3121 cacccgatct tccccgtgca ccggctgacg atcccctacc acgggctccc tggcaagggc
     3181 agcgcgttcc ggtgcatctt ccgcgtggcc gagaaactcg gagcgaaagc ctgcgccgtg
     3241 gtggactccg acctgcggag catcaacccc gagtgggtgc agctgctgat cgccccgcta
     3301 ctgcagcagg ggtacgacta cgtttcgccg tactacttcc ggcacaagta cgacgggacg
     3361 atcaccaaca gcatcgtcta cccgctgacg cgggcgctct acggacagcg cgtccggcag
     3421 ccgatcgggg gcgacttcgg cttctcgggc cgtctcgcct cgcacttcct ggcccagccg
     3481 gtgtgggaca ccgacgtcgc ccgctacggg atcgacatct ggatgaccac caccgcgatc
     3541 tgcggcggct tccgggtgtg ccagtcgttc ctcggcagca agctgcacga cgccaaggac
     3601 ccgggcgccg acctcagcgc catgctcgtc caggtgctcg gaacggtctt ctcgctgatg
     3661 gagacgcacg aggacgtgtg gcggcgggtc agcggctccg aggaaacgcc cctgatgggc
     3721 ttccgcttcg cggtcggcgt cgagccgatc gccgtcaaca cgcagcggat gctcgaccac
     3781 ttccgcaacg gcgtctcgaa cctccgcgac gtctggtcgc tcgtgctcgg gcccgccgac
     3841 ctgtggtgcc tcgaggaggc ggcgcgcgcc cgtcccgagg cgttccgcct cgacgacggg
     3901 ctgtggacgc ggatcgtgta cgacttcgcc gccgcgtacc atcaccggcg gatcgaccgc
     3961 caacacctga tccggtcgtt cctgccgctc tacatgggcc gggtggcgtc gttcgtgcgc
     4021 gaggtggccg agagcgacgc cccggccgtg gaggagcggc tcgaacaact gtgccgcgtg
     4081 ttcgaggaga acaaggcgca cctggcgaag cgctggtcct cgtgagggat gacggtcatg
     4141 ctagagaaac tgcaactggt gctgcaagag tcgtgggaga gcgcggccat cgagctgacg
     4201 cgcttcctcc cgcacctgct gggcgccgcc gtcctcatcg tagtcggcgc cggcctcgga
     4261 tggcttgccg gccgcatcac ccggtggctg ctctggcgcg ccagggccga cctcggggcc
     4321 gagcgcctcg gcatcatggg accggcccgg cgcctgggca tcgaaagccc gtcggccttc
     4381 atcgcccgcg tcgtccgctg gggcatcacg ctcgcggcgt tcgtgccggc cctctactcg
     4441 ctcgacgccc ggcttgcggc cgacctggtg cgccgcttcc tcctgtatct cccccacctc
     4501 gtggtcgccg tcggcctgct gtgggcggga ttcctggtca gccggttcgt cggccgaagc
     4561 gtgttgatcg ccgccgtcaa cacggggttc ggttcggcac gcctgctcgc cgctctcgcc
     4621 cgctggggga tcgtcctcgt ggtgcttgcg gtggccctcg agcacctggg catcgggcgc
     4681 gccaccgtgc tgaccgcctt cgccatcctc ttcgggggca tcacgctcgc cctggcgatc
     4741 gccgccggct tcggcagcca ggacgtcgtg cggcagtggt gggccgggcg cctgcagctg
     4801 cccgagaagc gggacgaaga gccgttcagg cactggtaga agggacctgg cggtccgcgc
     4861 ccccgacctc gcccgcccgc gccagcgcga ggcggaacac ggcggggccg aggaactggt
     4921 tgatcgcgac gagcgccacc accacggtct gaaggcggag gccccagccg gggaactgcc
     4981 gcgccaccac gatcgccagg cccaggacca cgcccgcctg ggacaccagc cccatccaga
     5041 ccagccggcc aggcggatcg ggcaccgagg gcagccgcgt cccgagcatc acgccgctcc
     5101 gcagcagcag catccgaaga ccggccaggg ccacggccag cacgccgacc tcggccagcg
     5161 catcgatctg gagcgacgcg ccggcagcag cgaagaacac cacgagcacc ggcggcgatc
     5221 cccgctcgac ggcatccttc aaggcgtcgc cttcgggcgg caccaggttc tcgacgacga
     5281 accccgccgc cagcgccgtg aggagcatct cggtgtgaag ggcccgcgag agctgcgtca
     5341 gcaccacgca caggaacagc aggacgatcg acacctcgcg gccgacgtgg tggaggtaca
     5401 aggcgaagag cgcgcccagg aggctgccga gcgccagcga cccgatcagt tcccacgcga
     5461 gcagtgccga cacgccgaca tcggcgcccg ggccggcccc ggccgcgtgc cgcagcagct
     5521 gcaccaccag ggtgaagccc acgatcaaca cgatctcggc catgatgacg accgcgagcg
     5581 tgatctcggt gagcgggccc cgcgcccggc tctcggcgat gaccgccatc gtcgccgccg
     5641 gggagaagct gacgacgatc gtcgtcagca cggcggcctg cgcgaaccgc agcagccccg
     5701 acgcggccgg atcgatcggc agccagggcc aggctgccca gagcaccgcc agcagcgcga
     5761 cgtacatcag gccgatggtc gtcccggcca gcgcgaggat tgccttgagg ctgcgccgca
     5821 gccgcccgag gttcatctcg aggccagcca ccagcgcgat cagcgccacc gccaggttgt
     5881 cgatcagctg gaggtcgcgc gccatcggcc gggtgatgat gttcagcaag tacgggccgc
     5941 acacgacccc gaagagcagg tagccggtca accgcggcaa ccgcgcgcgc tcgagcaggt
     6001 cgccggccag cgacgcggcg agcagcgcga tgccgagcgc aagcgccgtc gtgcggggcc
     6061 ctgccgcggg cgcggtcacc cgggccagcg ccagagccag gaggagcacg attccaaggg
     6121 ccgcgagccg ccgggtcacg gctcgccctc gtgc
//
LOCUS       DAOPQX010000211         6150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14008_length_6150_cov_4.990812, whole genome shotgun sequence.
ACCESSION   DAOPQX010000211 DAOPQX010000000
VERSION     DAOPQX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14008_length_6150_cov_4.990812"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKK95_11390"
     CDS             <1..537
                     /locus_tag="PKK95_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18867.1"
                     /translation="PPCIAIVLPSVHGVDGSAGDVSSALRELLTSYLTGPSLRTIALE
                     ARLQSQAVEEARQKGCDNILTATLTRKRSGGGALGRALGQAAGTAAWYVPGTASASGV
                     MARGAVIAGAQAVSTVASSTRAKDEMRLEYKFSTLDAAATARPATLQAKAQSDGEDIL
                     TPLAEKAAEAIAAQMIKQ"
     gene            617..2677
                     /locus_tag="PKK95_11395"
     CDS             617..2677
                     /locus_tag="PKK95_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC18868.1"
                     /translation="MQAAQLRWIVAFIALASVAAIPGERPAGAQQAPAGTTAVEALVV
                     DRDGRLVEGLGPSDFSVTVDGRTRPVLWVKRVSRGPGASADAASRQFGADSARLYAAE
                     PRRSVFIVIDEATMVRGDERNVVRAADALLERLGIDDLIAVVRIPVPAETLLTLTTER
                     PPIREALSTIVGRSAPATTPAETPGLAELSAMAEPSADPTIDRVPDLNKPPDGPVRAA
                     AGEMEKDATEEDLASARTALTTLDGLMASLQRLPGRKVVLLFSAGLFAPSQVRVEETA
                     ATAALARATVYAYGLRGVAQGPRRAPDLGPIETIARATGGSFAMLGRNPRRAVERTIA
                     ELSASYVLGIEADPSDTDGGRHAVRVEVARKGATARFAAWLPAAEPPADETPAAQEFA
                     TEAPADAASEAASPAAEKWKTAPPSTGAPAALARAGSSPSPREAELKLALARLFEYAD
                     AYERQYSMLVTEEEYLQTHRSGRTLTRADLLLVRPANRWVSFRDVFEVDGRPVRDRED
                     RLRRLFLDPTPEAQARLQSVMDESAKYNVGPVVRSINVPLFPLDILKSWNRERFDFKL
                     GRDADVDGLRAWRIDYTERERPTLVEGLEGEDIPIKGRFLVDQLTGAIVETTVALSKE
                     TVNGEIVVRYGRDPALGLWVPTQMRETYLQGGRRILMEGRAGYSKFRRFQVKTEESVT
                     LPVK"
     gene            complement(2724..3542)
                     /locus_tag="PKK95_11400"
     CDS             complement(2724..3542)
                     /locus_tag="PKK95_11400"
                     /inference="COORDINATES: protein motif:HMM:NF017006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0050113 - inositol oxygenase activity
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol oxygenase family protein"
                     /protein_id="HOC18869.1"
                     /translation="MDTSPTPAGPGTAQQKTPDQFRNYAAEARSTVKEFYRLNHANQS
                     LDFVLAKKKEYLGLNRKRMRVWEALEYLETLVDDSDPDTDLTQIEHAMQTAEAIRRAG
                     QPRWFIVTGLIHDLGKMMCLWGEPQWAVVGDTFPVGCAFSEKIVYPEFLELNPDRHHP
                     VYSTRLGIYEEGIGLDRVHLSWGHDEYIYHVVKGLLPIGALYALRYHSFYPWHKEGEY
                     EYLTDEQDRENLKWVKAFNRYDLYSKGDGKPDTKALRPFYEDLVDEFFPEPLNW"
     gene            complement(3669..4097)
                     /locus_tag="PKK95_11405"
     CDS             complement(3669..4097)
                     /locus_tag="PKK95_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18870.1"
                     /translation="MTTRHPPRFALAVLDRLLPGSSPLAGDLTEEFERGRSRAWFWWQ
                     VLAALGVAAIRRRDEIRPLRLVDLQPVEAQERSRRMLLHFDAVPLTGTNLPGVGGLTV
                     VALAVLVTLLAPIVWVAFVGSMLAGVALGLFFIARERRHS"
     gene            complement(4094..4426)
                     /locus_tag="PKK95_11410"
     CDS             complement(4094..4426)
                     /locus_tag="PKK95_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOC18871.1"
                     /translation="MGRQLLTDFELMILLAALRLEGDAYGVRIARQIEEVGRRRVLLG
                     AVYAALERLEANGLVSSTLGNPTSERGGRARRHFTVTPRGLRAVRDTRDAFVALWQGV
                     PQLEERSS"
     gene            4511..4651
                     /locus_tag="PKK95_11415"
     CDS             4511..4651
                     /locus_tag="PKK95_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOC18872.1"
                     /translation="MHDANLDYGDCFAYALAATTGESLLFKGDDFAKTDISRVRLGRE
                     QG"
     gene            4705..>6150
                     /locus_tag="PKK95_11420"
     CDS             4705..>6150
                     /locus_tag="PKK95_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOC18873.1"
                     /translation="MRKRILLAVALLAAAAAWWWSTRPEPAVRVSLIGPPETDAVPAI
                     NGHTAILISGRRVTPAGRVIRTQSYSWGMAVSPDQSRIALVRAGAIELVDLRNPGASV
                     RIPPYGADAPKELGDGTYMGVAFSPDGRRLYFGSANSGELKALDLAAVPGELAPPGAP
                     SALPGDLARHLVSIPIDGGGFRDSFLGDFVLDAAGTTIHALDQFNYRLVTIDAATRKV
                     VRSVRVGRNPFSVALAPDGRTAWVTNVGMFEYPLLPGVTEENRRTAGLTFPPYGIPSK
                     EAEEGTVAEGIVVPGLGSPNHPDAMSLFAVDLATGAVTARVKTGYLVGAERDGMKTIG
                     GSSPGGVVAGSRWVYVSNATNDTVSVIDASSGAIAAQVDLDVPGLERLRGVIPFGLAL
                     SPDEARLYVACAGLNAVAVIDTARRVVEGYIPAGWFTTSVAVARDGRTLFVSSAKGLG
                     SGPNGGAGFVAPSRGQHPGDIMQGTLQIVQVP"
BASE COUNT          957 a         2184 c         2108 g          901 t
ORIGIN      
        1 cctccctgta ttgcgatcgt gctgcccagc gtgcacggcg tcgacggcag cgcgggcgat
       61 gtgagcagtg cgctgcgcga gttgctgacg agctacctga caggcccctc tctcaggacg
      121 atcgcgctcg aagcccggct gcagtcgcag gcggtcgagg aagcgcgcca gaagggctgc
      181 gacaacatcc tcaccgccac gctcacgcgc aagagaagcg gcgggggcgc cctcggccgg
      241 gccctcggcc aggccgctgg cacggcggcg tggtacgtcc cggggaccgc gagcgcctcg
      301 ggcgtgatgg cgcgcggtgc cgtcatcgcg ggcgcgcagg ccgtttcgac ggtcgcgtcg
      361 tccaccaggg caaaggacga gatgcgcctc gagtacaaat tctcgacgct cgacgcggcg
      421 gcaacggcaa ggccagcgac cctccaggcc aaggcccaga gcgacggcga ggacatcctg
      481 accccgctgg ccgagaaggc ggccgaggcg atcgcggcgc agatgatcaa gcagtagccg
      541 gtcggaaccg tgccccggca cctgtgacgt ttctcacgct tcaggtacgg ccccaatccg
      601 tggcagactc tctgtcgtgc aggccgccca gctccggtgg atcgtcgcgt tcatcgcgct
      661 cgcctcggtc gccgccatcc cgggcgagcg gccggccggc gcccagcaag cgccggctgg
      721 taccacggcc gtcgaggcgc tcgtggtcga tcgcgacggg cgcctcgtcg aaggcctcgg
      781 gccgtccgac ttcagcgtca ccgtggacgg ccgtacccgg cccgtcctct gggtcaagcg
      841 cgtcagccgc gggcctggcg ccagcgccga cgcggcatca aggcagttcg gcgccgacag
      901 cgcaaggctc tacgccgcag agccccgccg cagcgtgttc atcgtcatcg acgaggcgac
      961 catggtgcgg ggcgacgagc ggaacgtcgt ccgcgccgcc gatgccctgc tcgaacgcct
     1021 cggcatcgac gacctgatcg cggtggtccg catccccgtt cccgctgaaa cgctgctgac
     1081 cctcaccacc gagcgccctc ccatccgcga agccctctcc accatcgtcg gccgatcggc
     1141 gccagcgacg acgccggcgg agacgccagg gctggccgag ctgtctgcga tggcggagcc
     1201 gagcgccgat ccgacgattg atcgcgtgcc cgatttgaac aagccccccg acgggccggt
     1261 gcgcgccgcc gcgggcgaga tggagaagga cgcgaccgag gaggacctgg cgagcgcgcg
     1321 tacggcgttg acgacgctgg acgggctgat ggcgtcgctg cagcggcttc cggggcgcaa
     1381 ggtggtgctg ctgttctccg ccggcctctt cgccccgtcg caggtgcggg tcgaggagac
     1441 ggccgccacc gcggcactcg cccgcgcaac ggtctacgcc tacggtctcc gcggcgtcgc
     1501 gcagggcccg cggcgcgcac ccgaccttgg ccccatcgaa accatcgccc gcgcgaccgg
     1561 tgggtcgttc gcgatgctcg gacgcaaccc gcggcgggcg gtcgagcgca ccatcgccga
     1621 gctgtcggcc agctacgtgc tgggcatcga ggccgaccct tccgacaccg acggcggccg
     1681 tcacgcggtg cgcgtcgagg ttgccaggaa gggtgccacc gcgcggttcg cagcctggct
     1741 tccggcggct gaacccccag cagacgagac gcctgcggcg caggagttcg caaccgaggc
     1801 gccagcggat gcggcatccg aggcggcctc gcccgccgcc gagaagtgga agaccgcgcc
     1861 tccctcgacc ggtgcgccag cggccctcgc ccgcgccggc tcgagccctt cgccccgcga
     1921 ggcggagctg aagctggccc tcgctcgcct gttcgaatat gccgacgcct acgagcggca
     1981 gtactccatg ctcgtgaccg aagaggagta cctgcagacg caccgcagcg ggcggacact
     2041 cacccgcgcc gacctgctgc tggtgcggcc ggccaaccgc tgggtctcgt tccgcgacgt
     2101 gttcgaggtg gacggccgcc cggtgcgcga ccgcgaggat cggctgcgtc gcctgttcct
     2161 ggacccgaca cccgaggccc aggcgcgcct gcagtcggtg atggacgaga gcgcgaagta
     2221 caacgtcggc ccagtggttc gctcgatcaa cgtcccgctc ttcccgctcg acatcctgaa
     2281 gagctggaac cgcgagcgct tcgacttcaa gctggggcgc gacgccgacg tggacggcct
     2341 gcgcgcctgg cgcatcgact acaccgagcg cgaacgcccc acgctcgtcg agggactcga
     2401 gggcgaggac atccccatca agggccgctt cctcgtcgat cagctcacgg gcgcgatcgt
     2461 cgagacgaca gtggcactgt cgaaggagac ggtgaacggc gagatcgtcg tgcgctacgg
     2521 gcgcgacccg gcgcttggcc tgtgggtccc gacccagatg cgcgaaacct acctccaggg
     2581 gggccgccgc atcctgatgg aagggcgggc cggctactcg aagttccgcc gcttccaggt
     2641 caagacggag gaaagcgtca cgctgccggt caagtgatcc cgacccttag cccccagccc
     2701 ccagccccca gcccccagcc cgctcaccag ttcagcggct cggggaagaa ttcgtcaacg
     2761 agatcctcgt agaagggacg aagcgccttc gtgtcaggct tgccatctcc cttcgagtag
     2821 agatcgtacc ggttgaacgc cttcacccac ttcaggttct cgcggtcctg ctcgtccgtc
     2881 aggtactcgt actccccttc cttgtgccag gggtagaacg agtgataccg cagcgcgtac
     2941 aaggcgccaa tcggcaggag gcccttcacc acgtggtaga tgtactcgtc gtggccccac
     3001 gagaggtgca cgcgatcgag gccgatgccc tcctcgtaga tcccgagccg cgtcgagtag
     3061 acggggtggt ggcgatccgg gttcagctcg aggaactcgg ggtagacgat cttctccgag
     3121 aaggcacagc ccacagggaa cgtgtcgccg acgaccgccc actgcggttc tccccagagg
     3181 cacatcatct ttccgagatc gtgaatcagc ccggtgacga tgaaccaccg cggctggccg
     3241 gcgcggcgga tcgcctcggc cgtctgcatg gcgtgctcga tctgcgtcaa gtcggtgtcc
     3301 gggtcggagt cgtctaccaa ggtttcgaga tactcgagcg cctcccagac gcgcatccgc
     3361 ttccggttca gcccgaggta ctccttcttc ttggcgagga cgaagtcgag cgactggttc
     3421 gcgtggttga gccggtagaa ctccttgacc gtgctgcgcg cctctgccgc gtagttcctg
     3481 aactggtcgg gggtcttctg ttgagcggtg cccggaccgg caggagttgg cgaggtgtcc
     3541 atgggggcct cctggtcagt gagcgtggtg tgcggatgga tccatgttac ccgcagtccc
     3601 ctccggctcc gtgatccttc gacatgcgcg cggcgaagcc agctgatcgg cgcggcgaat
     3661 cgtgcccgtc agctgtgcct tcgctcccgg gcgatgaaga acaggccgag ggcaacgcca
     3721 gcgagcatcg agcccacgaa cgccacccag acgatcggcg cgagcagggt caccagcacc
     3781 gcgagcgcca cgacggtgag cccgcccaca cccggaaggt tcgtcccggt cagcggaacg
     3841 gcgtcgaagt gaaggagcat ccggcgggag cgctcctgcg cctcgaccgg ctgcaggtcg
     3901 acgagacgca gaggccggat ctcgtcgcgc cgcctgatcg cggcgacacc gagcgcggcg
     3961 agaacctgcc accagaacca ggcacgcgac cgtccccgct cgaactcctc ggtgaggtcg
     4021 ccagccagcg gggagctgcc gggaagcagg cggtcgagca cggccagcgc gaaccgcgga
     4081 ggatgacgcg ttgtcatgac gacctctcct ctagctgggg cacgccctgc cacaaggcca
     4141 cgaacgcatc gcgcgtgtcc cgcacggcac gaagcccgcg cggagtcacc gtgaaatggc
     4201 gcctggcccg gccgccccgc tcggacgtcg ggttgccgag ggtcgaagac acgaggccgt
     4261 tcgcttcgag acgctcgagg gcggcataga cggcgccgag cagcacccgg cgacggccca
     4321 cctcctcgat ctgccgcgca atccgcacgc cgtaggcgtc gccttcgagg cggagcgccg
     4381 cgagcaggat catcagctcg aagtcagtga ggagttggcg gcccatttct tcataaatta
     4441 tgaactcgcc cacagaacga cgtcaagcac tgcgatacgg tgccctgcgc tacggacctg
     4501 aaggtgttcg atgcacgacg cgaacctcga ctacggcgac tgcttcgcct acgcgctggc
     4561 ggccaccacc ggcgagtctc tcctcttcaa gggcgacgac ttcgccaaga ccgacatctc
     4621 cagggtcagg ctgggacgcg agcagggctg aaaggacagc gggcgcgggt gacgcggagt
     4681 cgtatccccg cgtatgatcc gcgcgtgcgc aaacggattc tcctcgccgt cgcccttctt
     4741 gccgctgccg ccgcatggtg gtggtccacc cgtccggagc ccgccgtacg cgtttcgctg
     4801 atcgggccgc cggaaactga cgcggttccg gccatcaacg gccacacggc aatcctgatc
     4861 agcggccgcc gggtcactcc cgcgggacgc gtgattcgca cgcagtccta cagctggggg
     4921 atggcggtct cgcccgacca gtcgcgcatc gcgctcgtgc gcgccggcgc catcgagttg
     4981 gtcgacttgc ggaaccccgg cgcgagcgtt cgcatcccgc cttacggggc cgacgcccca
     5041 aaggagctgg gcgacggcac ctacatgggc gtcgcctttt cgccggacgg ccgccgtctc
     5101 tacttcgggt cggcgaactc gggcgagctg aaggcgctcg acctggcggc cgtgccggga
     5161 gagctggcgc ctcccggcgc gccatccgcc ctgcccggcg atctcgcgcg ccacctcgtc
     5221 tcgatcccga tcgacggcgg cggcttccgc gacagcttcc tcggcgactt cgtcctcgac
     5281 gccgccggca cgaccatcca cgcactcgat cagttcaact accgcctggt cacgatcgac
     5341 gcggcgaccc ggaaggtcgt gcggtcggtt cgggtgggac gcaacccgtt ctcggtcgcc
     5401 ctggccccgg acgggcggac ggcctgggtg accaacgtcg gcatgttcga gtacccgctg
     5461 ctgccgggtg tcaccgagga gaaccggcgc acggcgggcc tgacgttccc gccctacggc
     5521 atcccgtcga aggaggccga ggaagggacg gtcgcggagg ggatcgtggt gccggggctc
     5581 gggagcccga accatcccga cgcgatgtcg ctctttgcgg tggacctggc cactggcgcg
     5641 gtgaccgcgc gcgtgaagac cggttacctg gttggcgccg agcgcgacgg catgaagacc
     5701 atcggtggct ccagcccggg cggggtggtt gccgggtcgc ggtgggtcta cgtctccaac
     5761 gccaccaacg acacggtctc ggtgatcgac gcgagcagcg gcgcgatcgc cgcccaggtg
     5821 gacctcgacg tccccggcct cgagcggctg cgcggcgtga tccccttcgg cctcgcgctc
     5881 agcccagacg aggcgcgcct ctacgtggcg tgcgccggcc tgaacgcggt cgcggtcatc
     5941 gacacagcgc gccgggtggt cgaggggtac atcccggccg gctggttcac cacctcggtg
     6001 gccgtggcgc gcgacggccg cacgctcttc gtgtcgagcg cgaaggggtt gggctctggg
     6061 ccgaacggcg gcgccggctt cgtcgcaccc tcgcgcggcc aacaccctgg cgacatcatg
     6121 caaggcacct tgcagatcgt ccaggtcccg
//
LOCUS       DAOPQX010000212         6135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14047_length_6135_cov_5.512336, whole genome shotgun sequence.
ACCESSION   DAOPQX010000212 DAOPQX010000000
VERSION     DAOPQX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14047_length_6135_cov_5.512336"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PKK95_11425"
     CDS             <1..1330
                     /locus_tag="PKK95_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC18874.1"
                     /translation="PHEQERAAMAELEAQRDVALPFTVRWNRAERTATIDIQDETVHL
                     AEGQWSKWVPLEFRINFLMRLQGMAQLYLIKASDDLQLYISPVNWRPEKPVMPISAPG
                     SFSEDLYDRLGTFRTLGWAEATWPLNEDRIDEKAFMDDLYRAFDDRAQVILQQIDSRK
                     YDLVVGVIESTDRVQHMFWRFIDPTHPMYDAAQAAKWGDAIERVYRRADQLVAEVLEH
                     VEPGTDIIVLSDHGFHSFKHGVNLNTWLIEEGFMARQQQKVGDKNLDDMFGGGGQFWE
                     NVDWTRTKAYSLGLGQIYFNMRGREGQGIISEGEEYKRLSDELSARLMTMTDPKTGQR
                     IVTGVYKRDDVYSGPYMANAPDLQVGFADGYRVSWQTSLGGSPPGLLYDNMRKWSGDH
                     CSFDYRGIPGAIITTRKVTSGQVRIIDVAPTVLRYFGVPIPKEMDGKPLF"
     gene            1400..2530
                     /locus_tag="PKK95_11430"
     CDS             1400..2530
                     /locus_tag="PKK95_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOC18875.1"
                     /translation="MFRALMATAAACVAFGLAAPASQAQAPASDPAARADARIRALHQ
                     EADALARQQRSLLGDLRRLEVERDLRVEESRKLDAEAAALQAKMADTRTRIDAERAAI
                     AEIRPLLDRRLVEVYKLGRPGYGRLLLGARDVRDAGRVVRQTVALASMDQRRVDELRA
                     STDRLEKANAQLAEQAASLEGVREAARVAADKARRAADARAALIRQLDARRDLAAQMA
                     GELEMARQRLGQIVSSDPVEAALPIKPFRGSLDWPLRGRILSWFGQRRNPRVGTAANR
                     NGIEIAGAEGAPVRAVHEGRVIFADVFAGLGQLVIVDHGRLGYSLYGHLGSLGVQKGA
                     AVAHGQTVGTVGTSPAGNPALYFELRIDGRPVDPVEWLKESW"
     gene            2537..3697
                     /locus_tag="PKK95_11435"
     CDS             2537..3697
                     /locus_tag="PKK95_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOC18876.1"
                     /translation="MSARTRLFVFAVTAPVIAFALVGALLGQVWARETPYANLRVFEE
                     AVSLIANNYVEEVNLSKVMRGAMGGLAEGLDPDSAYLPPEAVREVDAGNGVGPADIGV
                     ALTRQYYLRVIAARDGSPAARAGLRAGDFLRVIGERPTREMSVWDGMRLLRGEPGSTV
                     SLLVIRGNAADPHPVEITREIPAAPIVSGRLIGTDVGYLRIPAFAAGIPAAVSARVAE
                     LRKAGASRLVVDVRDVADGPLDAGIATARLFVASGTLLHKETKGAGREAVTAGGGAAP
                     IAQPAVVLVNRGTSGAAELFAAALAGNKRAELVGERTHGRAGLQRLVRLNDGSAMLIT
                     NAWYLTPGGEPIHQKGLTPAHTVEVPDVEFGAPPPATDPILDKGLELVKDRK"
     gene            3896..4168
                     /locus_tag="PKK95_11440"
     CDS             3896..4168
                     /locus_tag="PKK95_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOC18877.1"
                     /translation="MNKQSLIEKIAKDTGVTKVSAAAALESALDGITRSLKKGEGITF
                     VGFGTFKTAVRKARTGRNPQTGAEVKIPKRRVVRFTAGKALKNAVK"
     gene            4328..4404
                     /locus_tag="PKK95_11445"
     tRNA            4328..4404
                     /locus_tag="PKK95_11445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4362..4364,aa:Val,seq:gac)
     gene            4534..>6135
                     /gene="thrS"
                     /locus_tag="PKK95_11450"
     CDS             4534..>6135
                     /gene="thrS"
                     /locus_tag="PKK95_11450"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOC18878.1"
                     /translation="MNHVTVTLPDGSRRTVPAGTTVRDLSADLPARVAKSALAAVVDD
                     KVVDLSHPLERDAAVRLVTPESPEAIDLYRHSTAHLMAAAVTSLFPGTQCGIGPATDE
                     GFFYDFVVPRPFVPEDLEAIESRMRELAKQDIPYERKMLPKDEAKRYFAERGEPLKVQ
                     LIEEKGGPVVSCYTIDDLFIDFCVGPHVPSTGRLKAFKLLSTSNAYWKGDARNQPMQR
                     VYGTAFFSDSELKAHLQRLEEAKRRDHRKLGRELGLFIGHPWAPGATFWLGKGTILYN
                     TLANYMRQVLYPSGYQEVKTPIILNKALWETSGHWEHYRENMFLVRSSDDEEMGLKAM
                     NCPAHFLVYASEMRSYKDLPLRLHEQTPLHRNEASGVLSGLTRVRQFSQDDAHCFVME
                     SQIAEEVERLLRLVQRVYGDFGLEYSVKLSTRPEQFMGEIATWDHAEASLRQALVAAG
                     QPYVVNEGDGAFYGPKLDFDITDAIGRKWQCATIQLDYQMPERFDLKYVGADNAEHRP
                     VVIHRAIFGSFERFIALLIEHYAGAF"
BASE COUNT          985 a         2169 c         2079 g          902 t
ORIGIN      
        1 gccccacgag caggagcgcg cggcgatggc cgagctcgag gcccagcgcg acgtcgccct
       61 gcccttcacc gtccgctgga accgggccga gcggacggcg accatcgaca tccaggacga
      121 gaccgtccac ctcgccgagg ggcagtggag caagtgggtg ccgctcgagt tccgcatcaa
      181 cttcctgatg cgcctccagg gcatggcgca gctctacctg atcaaggcgt cggacgacct
      241 gcagctgtac atctcgccgg tgaactggcg gcccgagaag ccggtgatgc cgatctcggc
      301 gcccggctcc ttctcagagg acctctacga ccgcctgggc accttccgca cgctcgggtg
      361 ggcggaggcc acctggcccc tcaacgagga ccggatcgac gagaaggcgt tcatggacga
      421 cctgtaccgg gccttcgacg accgggcgca ggtgatcctc cagcagatcg actcgcggaa
      481 gtacgacctg gtggtcggcg tcatcgagtc gaccgatcgc gtgcagcaca tgttctggcg
      541 cttcatcgac ccgacgcacc cgatgtacga cgcggcgcag gccgccaagt ggggcgacgc
      601 gatcgagcgg gtctaccgcc gcgccgacca gctcgtggcc gaggtgctcg agcacgtcga
      661 gccgggcacc gacatcatcg tgctgtcgga ccacggcttc cactcgttca agcacggcgt
      721 gaacctgaac acctggctga tcgaggaagg cttcatggcc cgccagcagc agaaggtggg
      781 cgacaagaac ctcgacgaca tgttcggcgg cggcggccag ttctgggaga acgtggactg
      841 gacgcgcacc aaggcgtact cgctcggcct cgggcagatc tacttcaaca tgcgcggccg
      901 cgagggccag gggatcatca gcgagggcga agagtacaag cgcctgtcgg acgagctgtc
      961 ggcgcggctg atgacgatga ccgacccgaa gaccgggcag cggatcgtga ccggcgtcta
     1021 caagcgcgac gacgtctact cggggcccta catggcgaac gcgcccgacc tgcaggtggg
     1081 gttcgccgat gggtaccgcg tgtcgtggca gacctcgctc ggcggctccc cgcccgggct
     1141 gctctacgac aacatgcgca agtggtcggg cgaccactgc tcgttcgact acaggggcat
     1201 cccgggcgcg atcatcacca cgcgcaaggt gacctccggg caggtgcgca tcatcgacgt
     1261 cgcccccacc gtcctcaggt actttggcgt gccgatcccg aaggagatgg acgggaagcc
     1321 gctgttctag acgcgagcct ggccaggccg tggtgaaggt gctagccccc agcccctcgc
     1381 gcctattaga atccccccca tgttccgagc gctgatggca accgctgcgg cgtgcgtggc
     1441 cttcggcctg gccgcgcccg cctcgcaggc ccaggctccg gcctcagacc ccgccgcccg
     1501 tgccgacgcg cggatccgcg ccctgcacca ggaggccgat gcgctcgcgc ggcagcagcg
     1561 cagcctgctc ggcgacctgc ggcgcctcga ggtcgagcgc gacctgcgcg tcgaggagag
     1621 ccggaagctc gacgccgagg ccgcggccct gcaggcaaag atggccgata ccaggacccg
     1681 gatcgacgcc gagcgggccg ccatcgccga gatcaggccg ttgctcgatc ggcgcctcgt
     1741 cgaggtctac aagctcgggc gccccggcta cggccgcctg ctgctcggcg cccgcgacgt
     1801 gcgcgatgcg ggccgcgtcg tgcgacagac ggtcgcgttg gccagcatgg accagcggcg
     1861 cgtggacgag ctgcgcgcgt ccaccgaccg cctcgagaag gccaacgccc agctcgctga
     1921 acaggcggcc agcctcgagg gcgtgcggga agcggcccgc gtggcggccg acaaggcccg
     1981 gcgggccgcc gacgcccgcg ccgccctcat ccgccagctg gacgcgcgcc gcgaccttgc
     2041 ggcgcagatg gccggcgagc tcgagatggc gcgccagcgc ctcgggcaga tcgtttcctc
     2101 cgatcccgtc gaggcggcgc ttccaatcaa gcccttccgc ggctcgctcg actggcccct
     2161 ccgggggcgc atcctctcgt ggttcggcca acgccggaac ccccgcgtcg gaacggccgc
     2221 caaccgcaac ggcatcgaga tcgccggggc cgagggggcc ccggtgcggg cggtgcacga
     2281 ggggcgcgtc atcttcgccg acgtgttcgc cgggctcggc cagctggtca tcgtcgatca
     2341 cgggcggctc ggctactcgc tctacggcca cctcggctcg ctcggagtgc agaaaggggc
     2401 cgcggtcgcc cacggacaga ccgtggggac ggtgggcacc tcgcctgccg gcaaccccgc
     2461 gttgtacttc gagttacgaa tcgacggccg gccggtcgat cccgtagaat ggctgaagga
     2521 gagctggtaa ccccccatga gtgctcggac acgcttgttc gtctttgccg tcaccgcccc
     2581 ggtgattgcg ttcgccctgg tgggcgcgct gctcggccag gtgtgggcgc gagagactcc
     2641 ttacgccaac ctgcgcgtct tcgaggaagc ggtctccctc atcgcgaaca actacgtcga
     2701 ggaagtgaac ctctcgaagg tgatgcgcgg ggcgatggga ggactggccg aaggcctcga
     2761 ccccgacagc gcgtacctgc cccccgaggc cgttcgtgaa gtcgatgccg ggaacggggt
     2821 aggaccagcc gacatcggcg tcgccctcac ccggcagtac tacctgcgcg tcatcgccgc
     2881 gcgcgacggc tcgcccgcgg cgcgcgcagg cctccgcgct ggcgatttcc tgcgggtgat
     2941 cggcgagcgg ccgacccgcg agatgtccgt ttgggatggc atgcggctgc tgcgcggcga
     3001 gccgggcagc acggtgtcgc tgctcgtcat ccgcggcaac gcggccgacc cgcacccggt
     3061 ggagatcacc cgcgagatcc cggcggcgcc aatcgtttcg gggcggctga tcgggaccga
     3121 cgtcgggtac cttcgcatcc ccgcgttcgc cgccggcatc cccgccgccg tgtcggcgcg
     3181 ggtggctgaa ctaaggaagg ctggcgcgtc ccggctggtg gtcgacgtcc gcgacgtcgc
     3241 cgacggcccg ctcgacgcgg gaatcgccac cgcgcggctc ttcgtcgcat cgggcacgct
     3301 gctgcacaag gaaaccaagg gcgccggccg cgaggcggtg accgccggcg gcggtgcggc
     3361 cccgatcgcg cagcccgcgg tcgtcctggt gaatcgcggc acatccggcg ccgccgaact
     3421 gttcgcggcc gccctcgcgg gaaacaagcg cgccgagctg gtgggcgagc gcacgcacgg
     3481 ccgcgcaggc ctgcagcgcc tcgtgcggct caacgacggt tcggcgatgc tgatcaccaa
     3541 cgcctggtac ctcacgcccg ggggcgagcc gatccaccag aagggcctga caccggcgca
     3601 caccgtcgag gtgccggacg tggagttcgg agcgccgccc ccggcgaccg atccgattct
     3661 cgacaagggc ctcgagctgg tgaaagaccg caagtagtac tccgcacgac gccgcggcga
     3721 tcactttcgt gaacgatctc gccgcggcgg ccgttcctgc gctggggcgt gaacgccacc
     3781 agcgcgctgt tcgctattga cacctgatca gcgctcgcgt ataaccccat ccggacgtgg
     3841 ctggcgtggt gagcaaaccg cgcggcattc ttcagcgaac gaggaggtgg gggtcatgaa
     3901 caagcagtcg ctgatcgaga agatcgcgaa ggacaccggg gtgacgaagg tcagcgcggc
     3961 ggcggcgctc gagtcggctc tggacgggat cacccgctcg ctgaagaagg gcgaggggat
     4021 taccttcgtt ggcttcggca cgttcaagac cgccgttcgc aaggcgcgca ccggacgcaa
     4081 cccgcagacc ggcgccgagg tgaagatccc gaagcggcgc gtggtccggt tcactgccgg
     4141 caaggcgctg aagaatgcgg tgaagtaggc gtcgtccgta ggggcgcagc ttgctgcgcc
     4201 cctaccggcc tcccgaatcc tgaacccgaa tcctgaaccc cgaatgctga atcccctcct
     4261 ccgttgaccg cccggcttgg ccgttgctat actagccact tctgtaagtg ccggcttttc
     4321 ttgatttagg cgcgtagctc agatggttag agcgcctgct tgacacgcag gaggtcggcg
     4381 gttcgagtcc gcccgtgcct accacctcgt tccgcggcag cggatccacg ccggagttcg
     4441 gccggccgga cgcgagggcg ttcagaaagc cgggtggatg cccggatgcc ccgagcgtga
     4501 tggctgagaa gacctgcctt tcctgatcac cggatgaacc acgtcactgt caccttgccc
     4561 gacggctccc gccgcaccgt ccccgccggg acgaccgtgc gcgacctgtc ggcggatctg
     4621 ccggcgcgcg tggcgaagtc cgccctcgcg gccgtcgtcg acgacaaggt cgtggacctc
     4681 tcgcatccgc tcgagcgcga cgcggcggtg cgcctggtca ccccggagag ccccgaggcg
     4741 atcgacctct accgccacag cacggcccac ctgatggccg cggcggtgac cagcctgttc
     4801 cccggcaccc agtgcgggat cggccccgcc accgacgaag gcttcttcta cgacttcgtc
     4861 gtcccgcgcc cgttcgtccc cgaggacctc gaggcgatcg agagccggat gcgcgagctg
     4921 gcgaagcagg acatccccta cgagcggaag atgctgccca aggacgaggc gaagcgctac
     4981 ttcgccgagc ggggcgagcc gctcaaggtg cagctgatcg aggagaaggg cggcccggtc
     5041 gtctcgtgct acacgatcga cgacctcttc atcgacttct gcgtcgggcc gcacgtgccg
     5101 tccaccgggc ggctcaaggc gttcaagctg ctctcgacct cgaacgccta ctggaagggc
     5161 gacgcgcgca accagccgat gcagcgcgtg tacggcaccg ccttcttctc cgactcggag
     5221 ttgaaggcgc acctgcagcg cctcgaggag gccaagcgcc gcgatcaccg gaagcttggc
     5281 cgcgagctgg gcctcttcat cgggcacccg tgggcgcccg gcgccacctt ctggctcggc
     5341 aagggcacga tcctctacaa caccctcgcc aactacatgc ggcaggtgct gtacccgtcg
     5401 ggctaccagg aagtcaagac gccgatcatc ctgaacaagg cgctctggga gacctccggg
     5461 cactgggagc actaccgcga gaacatgttc ctcgtccgct cgtccgacga cgaggagatg
     5521 ggcctgaagg cgatgaactg cccggcccac ttcctcgtct acgcgagcga gatgcgcagc
     5581 tacaaggatt tgccgctgcg cctccacgag cagactcccc tgcaccgcaa cgaggcgtcg
     5641 ggcgtcctct cgggcctcac ccgcgtgcgc cagttctcgc aggacgatgc gcactgcttc
     5701 gtgatggagt cgcagatcgc cgaggaggtc gagcggctgc tccgcctcgt ccagcgcgtc
     5761 tacggcgact tcgggctcga gtactcggtg aagctctcga cccgccccga gcagttcatg
     5821 ggcgagatcg ccacctggga ccacgccgag gcctcgctgc gccaggcgct cgtcgccgcc
     5881 ggtcagccgt acgtcgtgaa cgagggcgac ggcgccttct acggtccgaa gctcgacttc
     5941 gacatcaccg acgccatcgg gcggaagtgg cagtgcgcca ccatccagct cgattaccag
     6001 atgccggagc gcttcgacct caagtacgtg ggggccgaca acgccgagca ccgcccggtc
     6061 gtcatccacc gggccatctt cggcagcttc gagcgcttca tcgcgctgct catcgagcac
     6121 tatgccggcg ccttc
//
LOCUS       DAOPQX010000213         6050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14312_length_6050_cov_4.685405, whole genome shotgun sequence.
ACCESSION   DAOPQX010000213 DAOPQX010000000
VERSION     DAOPQX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14312_length_6050_cov_4.685405"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..619
                     /locus_tag="PKK95_11455"
     CDS             401..619
                     /locus_tag="PKK95_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18879.1"
                     /translation="MLSGTLDGASLPEMERLAGECASSGGSVVVDLSNVRLVDREAVR
                     FFSSGAGRRVKIVGCPAYLREWLRSEGR"
     gene            627..2081
                     /locus_tag="PKK95_11460"
     CDS             627..2081
                     /locus_tag="PKK95_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC18880.1"
                     /translation="MHWVAKSGGRAKGGRRRAGRLAWTLAVVGVLAVAPPVHAQQGEP
                     LSFPQALQRMISAHESLRAAGQEVGQRREEREARKGLYYPKVELSGQFTRIDEPIEID
                     LDPIRKAILTLHPQVPASRVPPFVQEVQGESFWKADIRATWPIYTGGKVAAANRAADA
                     RIADAEQQQRQTGQSLSSELVRRYFAVRLAGAAQEVRAAVLAGLDRHLFEARRLEEEG
                     LISKAERLHAEVARSEADRQVKRAGHDLAIARTALANILCEDAAGDPSSPLFIVDRLE
                     PLDAFQASASRHHPAFARLAAQQALASQAVAAERARWRPDVYLFAKRELHEDGLTLLD
                     PAWAAGVGATFTLFDGFARRHGAAAARLQLARVETLEARARRDVATLVEKRYREVERS
                     REQFDALAVALDLARENLRVRTRAFEEGLATSLEVVDARLALSRAELERLAAAYEFDV
                     ALADLLEASGQADRFEALRQQGKPVGAVLREQQR"
     gene            2087..3085
                     /locus_tag="PKK95_11465"
     CDS             2087..3085
                     /locus_tag="PKK95_11465"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC18881.1"
                     /translation="MRIVRAVLGLVAVAAVVAGLWAWASVVSRPVPEVLQGQVEGTLI
                     NVSGKLAGRLASIEVREGQRVARADVVARIESPEVHARLDQARAARDAAEAQREKAYR
                     GARAEEVRQARSMWERARHAAELAEKTFRRLERLNADGVVPAQRRDEAEAQWKTAREA
                     ENAARAALDMAEAGARAEDKAAAAALVDRAAGAISEVNAALAETTILAPSAGEVYRRN
                     AEPGEVVPAGYPVVTLLDPEDVWVVLQVREDKLAGACIGSPIAVRVPALGDRRLDLEV
                     SYVAPLGDFATWRPSSAQGGFDLKTFEVRARPRTPVAGLRPGMSAIVVSGLGVGGR"
     gene            3093..4253
                     /locus_tag="PKK95_11470"
     CDS             3093..4253
                     /locus_tag="PKK95_11470"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18882.1"
                     /translation="MLKVARRELGRIASDRYYLWLLVVLPLASYWLLASIFSAGVPRD
                     LPVAVVDQDHSTLSRQIVRAIDATPSMAVAARPGSIEAARALVLENQVYAVVVLPFGM
                     ERDARRGQAPAATGYLNAQALLPASLIRRDLRAAVATVSAGLEVRGRQARGEPPRAAL
                     ARFEPVRLDYHTLFNPRLDYVSYLLTALLPASLQIFVLLVAVYSVGGELKNGTAGSWI
                     EAAGGRAFRAAAGKLAPYALYFTALGLTMLAVLESRLGVPLGGSPALLGTATFLLVLA
                     YLAVGLALAVWFANLRLASSATAVYAAPAFAFVGITFPTMGMPVAGRLWGELLPLTHY
                     LRILVNQGIRNAPAGVSLPSLAALAAFAAAGVAASLWRLRRVASDERYWGRR"
     gene            4250..5389
                     /locus_tag="PKK95_11475"
     CDS             4250..5389
                     /locus_tag="PKK95_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18883.1"
                     /translation="MRTILAEAAAEFHRLRRDVGVLLVLVGAMFLYAALYPVPYWHEV
                     LEQVPIAIVDLDGSDLSRDLARMADAHQLLSVAARAATAEEAAGLVRRGEAGGYVVVP
                     PGFERQIRRGERAVVAAYVDAGYFLVYRQVLTGFSETVGTLSAGIEIRRLQGEGLAVR
                     QAVRARDPLPLSTRPLFNRTEGYATYVVPGVLVLILQQTLLVGIGMVGGTLRERPEPV
                     GRGTSRAAAKVLGRALVYFALYAVHALFYFGVVYALYGFPVKSQAADLASFVLPFLLA
                     AIFLALTLRPLFRTREASIHLLLWTSLPAIFLAGFAWPVEALPPWLAAAGRALPSTSA
                     IPGMIRLTQMGATLAQVRPEWWTLWALAGIYFATACAAARLRPES"
     gene            complement(5486..5692)
                     /locus_tag="PKK95_11480"
     CDS             complement(5486..5692)
                     /locus_tag="PKK95_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258313603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5663..5665),aa:Sec)
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HOC18884.1"
                     /translation="MVRIFGKDTUPYTQAAREDFRRRGVEVEYVNVKKDRAGLERMLA
                     HTAGRRDVPVIVEGEKVTIGWGGT"
     gene            complement(5711..>6050)
                     /locus_tag="PKK95_11485"
     CDS             complement(5711..>6050)
                     /locus_tag="PKK95_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOC18885.1"
                     /translation="IEDGEGHERHIRVLPVINPELTPVGRDTAEDWEGCLSLPHLRGR
                     VVRPDRVQLRALDRRGNRIELDLEGYTARVAQHEIDHLDGVLFIDRMKSLETLTFMDE
                     FAKFWAHPDD"
BASE COUNT          768 a         2124 c         2191 g          967 t
ORIGIN      
        1 tgcaccagca ccctcggcac ctcctgcacc agcaccctcg gcacctcctg caccatctgc
       61 acctcctgca cgatctaatt cctgatctcc ggcctccgcg cctccgcgcc tccgcgcctc
      121 cgcgttgaac gtggtcagga atgcagcgtg atacgcattt gagttctgtt cgtctctgtg
      181 tctctgtgcc tctgtggtga acgtgatctg aatgcagcgt gacggcgtga ctcgccgtga
      241 cgacgggtcg tgaattcacg atccgtcgtg acgcctcgag gcggtcgttg cctcaattcg
      301 cccgaaaagt gccgcgattc gacctggaac ggcgattgat gtgcatcgcg acggtatgtt
      361 caggatcgag gtcgccccaa catccggcgg agtgcggttg atgctgagcg gcacgctcga
      421 tggcgcgtcg ctccccgaga tggaaaggct cgccggggag tgcgcgtcga gcggcggttc
      481 ggtggtagtg gatctctcga acgtccgcct cgtcgatcga gaggcggtcc gcttcttctc
      541 gagcggagcg ggcaggcgcg tcaagatcgt cggctgcccg gcctacctgc gcgagtggct
      601 tcggtcggaa gggcggtagg cgcgtgatgc actgggttgc gaagagcggc gggcgggcga
      661 agggtggacg gcggcgggcg ggaagactcg cctggaccct ggcggtcgtt ggggtccttg
      721 ctgtggcgcc gcccgtgcac gcgcagcagg gcgagccgct gtcgttcccg caggcgctcc
      781 agcgaatgat ctcggcgcac gagtcgctgc gcgcggccgg ccaggaggtc gggcagcgcc
      841 gcgaggagcg cgaggcccgc aagggtctgt actaccccaa ggtagagctg tcggggcagt
      901 tcacccgcat cgacgagccg atcgagatcg acctcgaccc gattcggaag gcgatcctca
      961 cgctccaccc gcaggtgccc gcgtcgcgcg tacccccgtt cgtgcaggaa gtgcagggcg
     1021 agtcgttctg gaaggccgac atccgcgcta cctggccgat ctacacgggg gggaaggttg
     1081 ccgccgcaaa ccgggcggcc gacgcgcgga tcgccgacgc cgagcagcag cagcggcaga
     1141 cgggtcagtc gctctcgtcc gagctggtgc gccgctactt cgcggtgcgg ctggcgggcg
     1201 cggcgcagga ggtccgggcg gcggtgctgg cgggcctcga ccgccacctc ttcgaggcgc
     1261 gccgcctcga ggaggagggg ctcatctcga aggccgagcg gctgcacgcc gaggtggccc
     1321 gcagcgaggc cgaccggcag gtgaagcggg ccggccacga cctcgcgatc gcgcggaccg
     1381 ctctggccaa catcctctgc gaggacgcgg ccggcgaccc ctcgtcgccc ctcttcatcg
     1441 tcgatcggct cgagccgctc gacgcgttcc aggccagcgc ctcgcgccac catccagcct
     1501 tcgcgcgcct tgccgcgcag caggccctgg cgagccaggc ggtggccgcc gagcgggctc
     1561 gctggcgccc cgacgtctac ctgttcgcga agcgcgagct gcacgaggat ggcctgacgc
     1621 tgctcgaccc ggcatgggcg gcaggcgtcg gcgccacttt taccctgttc gacggcttcg
     1681 cccgaaggca cggcgcggcg gccgcccggc tgcagctggc gcgcgtcgag acgctcgagg
     1741 cacgtgcccg ccgcgacgtc gccacgctgg tcgaaaagcg ctaccgggag gtcgagcggt
     1801 cgcgcgagca gttcgacgcg ctggccgtag cgctggacct cgctcgcgag aacctgcgcg
     1861 tgcgcacccg ggccttcgaa gaggggctcg ccacgtcact cgaggtggtc gatgcccgcc
     1921 tggcgctctc gcgcgccgag ctcgagcgcc tggccgcggc ctacgagttc gacgtcgcgc
     1981 tggccgacct gctggaggcc agcgggcagg ccgatcggtt cgaggcgctg cggcagcagg
     2041 gcaagccggt cggcgccgtc ctgagggagc agcagcggtg aagcacatgc gaatcgttcg
     2101 cgccgtcctc ggcctggtgg ccgttgcggc cgtcgttgcc ggattgtggg cctgggcctc
     2161 ggtcgtgtcg cggcctgtgc ccgaagttct gcagggacag gtcgagggca cgctgatcaa
     2221 cgtgtcgggc aagctcgccg gccgccttgc atcgatcgaa gttcgcgaag ggcagcgcgt
     2281 tgcgcgcgcc gacgtcgtgg cgagaatcga aagccccgag gtgcacgccc gactcgacca
     2341 ggcgcgcgca gcccgcgatg ctgccgaggc gcagcgcgag aaggcgtacc gcggcgcgcg
     2401 ggcggaagag gtccgccagg cgcgcagcat gtgggagcga gcgcgccacg ccgccgagct
     2461 ggccgagaag accttccggc ggctcgagcg cctcaacgcc gacggcgttg tgcccgcgca
     2521 gcgccgcgac gaggccgagg cgcagtggaa gaccgcccgc gaggccgaga atgccgcccg
     2581 ggccgccctc gacatggccg aggccggggc gcgcgccgag gacaaggcgg cggccgcggc
     2641 actggtggac cgtgcggcgg gcgccatctc cgaggtgaac gccgccctgg ccgagacgac
     2701 gatcctggcg ccctccgccg gggaggtgta ccgcaggaac gccgagcctg gcgaggtggt
     2761 gccggcgggc taccccgtgg tgacgctgct cgacccggag gacgtctggg ttgtcctgca
     2821 ggtgcgcgag gacaagctgg ccggagcctg catcggttcg ccaatcgccg tgcgcgtgcc
     2881 ggcgctgggc gaccggcgcc tggacctcga ggtgtcgtac gtcgcgccgc tcggcgactt
     2941 cgccacctgg cggccgagca gcgcgcaggg tgggttcgat ctcaagacgt tcgaggtccg
     3001 cgcacggccg cgcacgccgg ttgccggcct tcggcccggc atgagcgcga tcgtcgtgag
     3061 cggcctcggt gtgggcggcc ggtgagggag acgtgctgaa ggttgcgcgg cgggagcttg
     3121 gccggattgc ctccgaccgc tactacctgt ggctgctcgt ggttctgccc ctcgcgtcgt
     3181 actggctcct cgcctcgatc ttctccgccg gagtgccgcg cgacctcccc gtggcggtgg
     3241 tggaccagga ccacagtacg ctgtcgcgcc agatcgtgcg ggcgatcgac gccactccgt
     3301 ccatggcggt ggcagcgcgg cccggcagca tcgaggccgc gcgcgcgctg gtcctcgaga
     3361 accaggtgta cgcggtggtc gtgctgccgt tcggcatgga gcgtgacgcc aggcgtggcc
     3421 aggcccctgc ggcaaccggt tacctcaatg cgcaggcgct gctgcctgcc agcctgatca
     3481 ggcgcgactt gcgggccgcg gtcgccaccg tctcggcggg cctcgaggtg agaggtcgcc
     3541 aggcgcgggg ggaaccgccg cgcgcggcgc tcgcccgctt cgagccggtg cgcctcgatt
     3601 accacacgct cttcaatccg cgcctcgact acgtctcgta cctgctgacg gcgctgctgc
     3661 ccgcctcact ccagatcttc gtcctgctgg ttgcggtgta ctcggtggga ggcgagctga
     3721 agaacggaac ggcgggttcg tggatcgagg cggcaggcgg gcgcgcgttc cgcgccgccg
     3781 ccggcaagct ggcgccgtac gccttgtact tcacagccct cggcctgacg atgctggcgg
     3841 tgctcgaaag ccggctgggc gtcccgctcg gcggcagccc ggccctcctc ggcacggcca
     3901 cgttcctcct cgtactcgcc tacctggccg tagggctcgc gctcgcggtc tggttcgcga
     3961 acctgcgtct cgccagcagc gcgacggcgg tctacgcggc gcccgcgttc gcgttcgtcg
     4021 ggatcacctt ccccacgatg ggcatgccgg tcgcgggccg cctgtggggg gagcttcttc
     4081 ccctcaccca ctacctgcgg atcctggtga accagggcat ccggaatgcg ccggccggcg
     4141 tctcgctgcc gtcgttggcg gccctcgcgg ccttcgccgc ggccggcgtc gcggcgtcgc
     4201 tgtggcggct ccgccgcgtg gccagcgacg agcgctactg ggggcggcgg tgagaacgat
     4261 tctcgccgag gccgcggctg agttccacag gctgagacgc gacgtcggcg tcctgctggt
     4321 gcttgtcggg gcgatgttcc tgtacgcggc gctctacccg gtcccctact ggcacgaggt
     4381 gctcgagcag gtgcccatcg cgatcgtgga cctcgacggc agcgacctca gccgcgacct
     4441 ggcgaggatg gccgacgcgc accaactgtt gtcggttgcc gcgcgtgccg ccaccgcgga
     4501 ggaggccgcc ggcctggtgc ggcgaggaga ggctggcggc tacgtggtgg tgccgccggg
     4561 attcgagcgg cagatccggc gcggcgagcg ggcggtcgtg gccgcctacg tggatgccgg
     4621 ctatttcctc gtctaccggc aggtgctgac ggggttctcc gagacggtgg gcacgctctc
     4681 ggcgggcatc gagatccggc gcctgcaggg cgaggggctc gccgtgcgtc aggccgtgcg
     4741 cgcgcgcgac ccgctgccgc tctccacccg gccgctcttc aaccggaccg agggctacgc
     4801 cacctacgtc gttcccggtg tgctggtgct catcctccag cagaccctgc tcgtcggcat
     4861 cggaatggtg ggcggaacgc tgcgggagag gccggaaccg gtcggccgcg gcacgtcccg
     4921 cgcggcggcg aaggtgctgg gccgggcgct ggtctacttt gccctgtacg ccgtccacgc
     4981 cctcttctac ttcggcgtgg tctacgccct ttacggcttc ccggtgaagt cgcaggcggc
     5041 cgacctcgcg tcgttcgtcc tgcccttcct gctcgccgcc atcttcctcg cgctcacgct
     5101 tcgcccgctc ttccgaacgc gcgaggcctc gatccacctg ctgctgtgga cctcgctgcc
     5161 ggcgatcttt cttgctggct tcgcctggcc cgtggaggcg ctgccgccgt ggctggcggc
     5221 ggcggggcgt gcgttgccca gcacgtcggc catcccgggc atgatcaggt tgacgcagat
     5281 gggggcgaca ctggcgcagg tgcgccccga gtggtggacg ctctgggcgc tggccgggat
     5341 ctacttcgcg accgcctgcg cggccgccag gctgagaccc gagtcttgag cttctggacc
     5401 tcgcccgcct cctggcaggc ggcgcgaagc cagacaaagg gggggcgttc ttccgctgcc
     5461 gccggcagcg gctccttcaa acccctcagg tcccgcccca gccgatcgtg accttctccc
     5521 cctcgacgat gaccgggacg tcgcggcggc ccgcggtgtg ggccagcatc cgctcgaggc
     5581 cggcgcggtc cttcttcacg ttgacgtact cgacctccac gcctcgccgc cggaaatcct
     5641 cacgggcggc ctgggtgtac ggtcaggtat ccttgccgaa gattctgacc atgtggtcct
     5701 cgccctcctc ctaatcgtcc gggtgtgccc agaacttcgc gaactcgtcc atgaaggtga
     5761 gggtctcgag cgacttcatc cggtcgatga acagcacgcc gtccaggtga tcgatctcgt
     5821 gctgcgcgac ccgcgccgtg tacccttcga ggtccaactc gattcggttc ccgcggcggt
     5881 cgagggcgcg caactgcacc ctgtccgggc ggacgacccg gccacgcagg tgcggcaaac
     5941 tcaggcaccc ctcccagtcc tcggccgtat cgcggccgac cggcgtcagc tcggggttga
     6001 tcacgggcag cacgcggatg tgccgttcgt ggccctcgcc gtcctcgatc
//
LOCUS       DAOPQX010000214         6002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14456_length_6002_cov_4.778207, whole genome shotgun sequence.
ACCESSION   DAOPQX010000214 DAOPQX010000000
VERSION     DAOPQX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14456_length_6002_cov_4.778207"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="PKK95_11490"
     CDS             complement(<1..1738)
                     /locus_tag="PKK95_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18886.1"
                     /translation="MRTVSTRLVPTLLGALCLAFPAHLPAQAPPAAQAPAESRYQLPP
                     KVIVDILDARPLPAVIVSPSRQQLALLDRPSMPSIAELSQPMLRLAGTRINPATNGPQ
                     RSQGITGITLKSIADGKDTPVVVPPEPRLDPIGFSPGGRRLAFTQTRPGGIELWVADT
                     ATGQAKAVTVAALNATLGPPCQWVDDNSLLCEFVVASRGAAPKPPDVPTGPNVQENEG
                     KAAPVATYEDLLKSAHDEALFEYYFTGQLAFVDAATGRRTPVGSPGTFDEVIVSPDGQ
                     HLIVARLKRPFSRLVPARGFPKDVEIWSRTGRLEKRIASLPAAESVPMLGVPTGPRAY
                     QWNQSAPATAVWVEALDEGNLRNQVPKRDRVMTVEAPFSGDPAEWLRLDHRFAGLSWT
                     EDGTALVSEFERGKRMRTTWAVPAGSSHPRRLWQVSAEDRYRDPGTPIVVQKGGARLI
                     QQSGGAVFLAGAGASPKGDFPFLDRFDLATGNTERLFQAQGESYEAIVAVLDGDAKTL
                     LTRYETAKEPPNYFVCTRATGERRALTRFTDPAPQLAGVERRFLTYPRKDGVQLSATL
                     YLPPGYKEGQRLP"
     gene            complement(2050..3870)
                     /locus_tag="PKK95_11495"
     CDS             complement(2050..3870)
                     /locus_tag="PKK95_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC18887.1"
                     /translation="MAVDGADWRVIDEHIAAGRLPAFARLRKAGTQGILKTDPPRLSP
                     IAWTTVATGRPPEEHGVLDSMVDGGDGSLVPVHAGVRRVKAAWEIWSEASRSVLVLGW
                     PVTWPADRVRGMLASDRLPTGVAAGAPGLVYPPEALAKVVRHVVAPSSLDMEALTAFG
                     DAASAGPAWVSRLRFGLAAARSWRGIAAAALEAGQPDLFAVCFELVDTVSHLAVRDRS
                     RGERAIGAAYQEVDAALAHLAARLHPATLVVVLSAHGFYTADAGIREDPLAAAFDAAA
                     AWHRPYGIFAAADAATVAGTGRPGSPATLGIVSPLDVLPTLLAHAGLPVAADMPGKVL
                     AALTPAGPQSRVPTHGPRAEPEPPRPDEAGDKAEAGRPGDLGGPPLAAGMTPLARLHL
                     AEILYRKGDYRGAIRELEAAGRKDPLDGRAWLWLGRCHAALGRIDEALRAYDALLGAK
                     RASGGARATALLEATTLALGAGRVPAALERVDRAPAAIGREPAALVARGDAAEAEGRR
                     QTAERSYEAALNVAPSDVDAATRLIDLLIADGRADTARVLAARLAQAYPSSPRHLALA
                     GRAALAVKSHTEAARWFELALVLAPDDAELLALLDSTRKR"
     gene            4145..>6002
                     /locus_tag="PKK95_11500"
     CDS             4145..>6002
                     /locus_tag="PKK95_11500"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOC18888.1"
                     /translation="MTSRALSVVRRPLLAVCVLTLFAVPAAAQQDPLLPLRVARAPVI
                     DGVLDDSAWEEAPKIAGFKTWLPDYGKEMGDGTVVWYAYDEENLYFAFRAYDREPGRI
                     KASVASRDSIRPDDWICINLDSFNDQQALYGLYVNPLGIQMDSRYAAGQEDLGFDAVW
                     YSAGRIDAEGYTVEIRLPFKSIRYSTGNPVTMSVVFERFVSRSREDGTWPPLDPKAGG
                     NFLIQGQPIVFRDIRHYRLFEVLPDATYSRRDAASGGRLRNEASDGELGVSLKYGLTP
                     QLTADLTYNPDFSQVEADAGQIDVNLRHPLFFAEKRPFFLEGQEVFNLGGPAQHGTLQ
                     SVLHTRNIVNPVAGAKITGKLAPRDTLAALYSVDEQATADGNAQFSSVRYKRALDQDA
                     YLGAFYVGREQGAIHNRLVGADGLLRFDRSTSIGFYGFGSSTRHEGDASAAAGHALGA
                     DFTRDTRAFTLYFNAFDISKAFAADSGYLTRNGVTSVTLFAAPKLYPRRGPVTRVQLS
                     VTSQQTRDAFAGIWESYNEVGVMTWLRRAASLSVEYHYASEVFNAREFGTSGIGASGS
                     IQLTKQVRLQGNLRYGDAIYYAADPFGGRATSGSLSLTYQPSEQWNQALTLNY"
BASE COUNT          872 a         2125 c         2147 g          858 t
ORIGIN      
        1 ccggcagccg ctgtccctcc ttgtatcccg gcggcaggta cagggttgcg ctcagttgca
       61 cgccgtcctt gcgggggtag gtgaggaacc gccgctcgac ccccgccagc tggggtgccg
      121 ggtcggtgaa ccgcgtcagt gcgcgccgct cgccggttgc gcgggtgcac acgaagtagt
      181 tgggcggctc cttcgcggtt tcgtaccgcg tcagcaaggt cttcgcatcg ccgtcgagca
      241 cggccacgat cgcctcgtag ctctcgccct gcgcctggaa cagcctctcc gtattcccgg
      301 tcgcgaggtc gaaccggtcg aggaagggga agtcgccctt cggggaggcg cccgcgcccg
      361 cgaggaagac ggcgccgccc gactgctgga tcagccgcgc gccgcccttc tggacgacga
      421 tgggagtgcc gggatcgcgg taccgatcct cggcgctcac ctgccagaga cggcggggat
      481 ggctcgagcc cgccgggacc gcccacgtcg tccgcatccg cttgccgcgc tcgaactcgc
      541 tcaccagcgc cgtgccgtcc tcggtccagc tgagcccggc gaaccggtgg tcgaggcgca
      601 gccactcggc gggatcgccc gagaacgggg cctccacggt catcacccgg tcgcgcttcg
      661 gcacctggtt tcgcaggttg ccctcgtcca gggcctccac ccagacagcg gttgccggag
      721 cgctttggtt ccactggtag gcgcgcggcc cggtcgggac gccgagcatg ggaaccgact
      781 cggcagccgg gagcgaggca atccgcttct ccagccggcc cgtcctcgac cagatctcga
      841 catccttcgg gaagccgcgc gcgggaacca gccgcgagaa ggggcgcttc agccgcgcga
      901 cgatgaggtg ctgcccgtcc ggcgagacga tgacctcgtc gaaggtcccc ggcgagccga
      961 ccggcgtccg gcggcccgtg gcggcgtcga cgaaggccag ctgcccggtg aagtagtact
     1021 cgaagagcgc ctcgtcgtgc gcgctcttga gcaggtcctc gtaggtggct accggcgcgg
     1081 ccttcccctc gttctcctgc acgttcggac ccgtggggac gtcgggcggc ttcggcgccg
     1141 ctccccggct cgccacgacg aactcgcaca acagtgagtt gtcgtccacc cactggcacg
     1201 gggggccgag cgtggcattc agcgcggcca cggtgacggc cttcgcctgg ccggtggcgg
     1261 tgtcggcaac ccacagctcg atgccgccag ggcgggtctg ggtgaacgcg aggcggcggc
     1321 cgcccggcga gaacccgatg gggtcgagcc tgggttcggg cggcacgacg accggcgtgt
     1381 ccttgccgtc ggcaatcgac ttcagcgtga tgccggtgat cccctggctg cgctgcggcc
     1441 cgtttgtcgc cgggttgatg cgcgtgccgg ccagccgcag catcggctgc gacagctcgg
     1501 cgatcgacgg catcgacggg cggtcgagca gcgcgagctg ctggcgcgac gggctgacga
     1561 tgaccgcggg cagcgggcgg gcatcgagga tgtcgacgat gacctttgga ggaagctggt
     1621 agcgcgactc ggccggggcc tgcgcagcgg gcggcgcctg cgcgggcagg tgggcgggga
     1681 acgcaaggca gagcgccccc agcagcgtcg gcacgagtcg ggtgctcacg gtgcgcatgc
     1741 gcagtctcct caggaggatg ccaggatccg ggtcggcggg ccgattctac cacctgctgc
     1801 gacggcacca cggaggctgc cggacgaggc aaccacaaag aacacaaaga tcacaagaac
     1861 tgcgtattcc ccggcccagc tatcgacaag cctcaggcgc cggctttcgg cggccgtccc
     1921 gcgcagagtc gagggacggc ccgaaggccg gcgcgagcga ggcagacctt ttcgcagccg
     1981 ccttcatgtt ctttgtgtcc tttgcggttc aaattcgtgg acgggtcatg ccgagaacgg
     2041 ccgccgcggt catcgcttgc gcgtgctgtc gagcagcgcg agcagctcgg catcgtccgg
     2101 cgccaggacg agcgccagct cgaaccaccg cgcggcctcg gtgtggctct tcacggcaag
     2161 cgcggcgcgg ccggcgagcg ccaagtgccg cggcgacgag gggtaggcct gggcgaggcg
     2221 ggctgcgagc acgcgcgccg tatcggcgcg gccgtcggcg atcaacaagt cgatcagtcg
     2281 ggtcgcggca tccacgtcgg acggcgcgac gttcagcgcg gcttcgtacg agcgctctgc
     2341 ggtctgtcgc cggccctcgg cctcggcggc gtcgccccgc gcgaccagtg cggccggctc
     2401 gcggccgatc gcggccggcg cgcggtccac ccgctcgagc gcggcgggca cccggccggc
     2461 gccgagcgcg agcgtggtgg cctcgagcag ggcagtggcc cgggcgccac cgctggcccg
     2521 cttcgctccc agcagcgcgt cgtacgcgcg cagcgcttcg tcgatgcggc cgagcgccgc
     2581 gtgacagcgg ccgagccaca accatgcccg accgtccagc ggatctttgc gtccagccgc
     2641 ttcgagctcg cggatggcgc cccggtaatc ccccttgcgg tagagaatct ccgccaggtg
     2701 cagtctcgcg agcggtgtca tgccggcggc gaggggcggg ccgccgagat cgcccgggcg
     2761 cccggcctcg gccttgtcgc cggcctcgtc tggccgcggc ggctcgggtt cagcgcgagg
     2821 cccgtgggtg ggcacccgcg actgcgggcc ggccggggtg agggcggcca gcaccttccc
     2881 cggcatgtcg gcggccaccg gcagacccgc gtgcgcgagc agtgtcggca gcacgtcgag
     2941 aggggacacg atgccgagcg tggccgggga gccgggccgg ccagttccgg cgaccgttgc
     3001 ggcatcagcc gccgcgaaga tgccgtacgg ccgatgccag gcggcggccg cgtcgaacgc
     3061 cgcagccaac gggtcttcgc ggatcccggc gtcggccgtg tagaagccgt gcgccgacaa
     3121 cacgacaacg agcgttgccg ggtggaggcg cgcggcaagg tgggccagcg ccgcgtccac
     3181 ctcctggtag gccgcgccaa tcgctcgctc gccccgcgaa cggtcgcgca ccgcgaggtg
     3241 cgagacggtg tccaccagtt cgaagcagac ggcaaacagg tcgggctgcc ccgcctcgag
     3301 cgccgcggcg gcgatccccc gccagctcct cgccgccgcg agcccgaacc gcagcctcga
     3361 gacccacgcg ggaccggcgg atgccgcgtc gccgaacgcc gtgagagcct ccatgtcgag
     3421 cgacgagggg gccacgacgt gccgtaccac cttcgccagc gcctccgggg gatacacgag
     3481 gccgggcgct cccgccgcta cgccggtcgg caggcggtcg ctggcaagca tgccgcgcac
     3541 gcggtcggcc ggccaggtca ccggccaccc caggacgagc accgaccggg aggcctccga
     3601 ccagatctcc cacgcggcct tgacgcgccg gactcccgcg tgcacgggaa ccagcgaccc
     3661 gtcccctccg tcaaccatgg agtcgagaac gccgtgctcc tcgggcggcc ggccggtcgc
     3721 caccgtcgtc caggcaatcg gggaaagccg gggagggtcg gtcttgagga tgccctgcgt
     3781 tcccgctttc ctgaggcggg cgaaggccgg caggcgcccg gcggcgatgt gctcgtcgat
     3841 gacgcgccag tcggcgccat cgaccgccac gatggcgatg cgactcgcgg cagcctggcc
     3901 ggttctcgcc tgatcgggat tcgtctgact ggggttctga ccggaacagg cttccggtcc
     3961 ttggctgtgc gccccgagac acacggcggc cgcgagcatc gtgagcacgg cgccgatgaa
     4021 tcggggcggc cgtgatgcca tcgcggaccg acgtcagtat cctccaagcg cggccggggg
     4081 tctacccgcc ttgcgtgccg tgctagtttc tttcctcccg cccccgagga ctggattcgc
     4141 ctgaatgacc tctcgagccc tgtcggttgt gcgacggccg ctgctggccg tgtgcgtgct
     4201 cacgctgttc gcggtgcctg cggccgcgca gcaggacccg cttctaccgt tgcgagtggc
     4261 gagggcgcct gtcatcgacg gcgtgctcga cgactcggcg tgggaggagg cgccgaagat
     4321 cgccgggttc aagacgtggt tgcccgacta cgggaaggag atgggcgacg gcacggtggt
     4381 ctggtacgcc tacgacgagg agaacctcta cttcgccttt cgcgcgtacg accgggagcc
     4441 cggccggatc aaggcgtcgg ttgccagccg cgactcgatc cggcccgacg actggatctg
     4501 catcaacctc gactccttca acgaccagca ggcgctgtac ggcctctacg tcaaccccct
     4561 gggcatccag atggacagcc gctacgcggc gggccaggag gacctcgggt tcgatgccgt
     4621 ctggtacagc gcgggccgca tcgacgccga ggggtacacc gtcgagatcc gccttccctt
     4681 caagagcatc cggtacagca ccgggaaccc cgtgaccatg agcgtagtgt tcgagcggtt
     4741 cgtcagccgg agccgggaag acggcacctg gccgccgctc gacccgaagg cggggggtaa
     4801 cttcctcatc caggggcagc cgatcgtctt ccgggacatt cgccactaca ggcttttcga
     4861 ggtcctgccc gatgccacct acagccggcg cgacgcggcg agcggcgggc ggctgcggaa
     4921 cgaggcctcg gacggcgaac tgggggtgtc gctcaagtac ggcctgacac cgcagctgac
     4981 ggccgacctc acctacaacc ccgacttcag ccaggtcgag gccgacgccg gccagatcga
     5041 cgtgaacctg cgccacccgc tgttcttcgc cgagaagcgg ccgttcttcc tcgaggggca
     5101 ggaggtgttc aacctgggcg gcccggcgca gcacggcacg ctgcagtcgg tccttcacac
     5161 caggaacatc gtcaacccgg ttgcgggcgc caagatcacc ggcaagctcg ccccgcgcga
     5221 caccctcgcg gcgctctact cggtggacga gcaggcgacg gcggacggga atgcgcagtt
     5281 ctcgtcggtc cgctacaagc gggcgctcga ccaggacgcc tacctcgggg cgttctacgt
     5341 cgggcgtgag cagggggcga tccacaaccg cctggtcggc gccgacgggt tgttgcgctt
     5401 cgaccggtcc acctccatcg ggttctacgg gtttgggtcg agcacgcgcc acgagggaga
     5461 tgcgtccgcc gcagccgggc acgcgctggg ggccgacttc acccgcgaca cccgcgcctt
     5521 caccctctac ttcaacgcgt tcgacatctc gaaggcattt gccgccgaca gcgggtacct
     5581 cacgcggaac ggcgtaacgt ccgtcaccct gttcgcggcg ccgaagcttt acccgaggag
     5641 gggaccggtg acgcgcgtcc agctgtcggt cacctcccag cagacccgcg acgccttcgc
     5701 gggcatctgg gagagctaca acgaggtggg ggtcatgacc tggctgcgcc gggcggccag
     5761 cctgtcggtc gagtaccact acgcgagcga ggtcttcaac gcacgcgagt tcggcacgag
     5821 cgggatcggg gcgtccgggt cgatccagct caccaagcag gtgcgcctgc agggcaacct
     5881 gcgctacggc gacgcgatct actacgcggc cgatcccttc ggcgggcggg ccacctcggg
     5941 ctcgctgtcg ctgacctatc agccctcgga gcagtggaac caggcgctca cgctgaacta
     6001 cg
//
LOCUS       DAOPQX010000215         5984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14512_length_5984_cov_3.678192, whole genome shotgun sequence.
ACCESSION   DAOPQX010000215 DAOPQX010000000
VERSION     DAOPQX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14512_length_5984_cov_3.678192"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..682)
                     /locus_tag="PKK95_11505"
     CDS             complement(140..682)
                     /locus_tag="PKK95_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOC18889.1"
                     /translation="MADSLIDIPDKPAFKAAEVCDLVQIPPYVLRSWEKEFPNLGVVP
                     RAGAPRVYRRGDVEQILRIKQLVFSEGLTLAGARRRLEGEPPPEPEPDFPLPEEVKAR
                     VGRVKQELKSLLQLLGPEREATPPPPSTSTWPPARASVPLRREDDEPALPLLDAEAET
                     TTPPPAAAMPGGTKRKRGRK"
     gene            complement(701..2095)
                     /gene="der"
                     /locus_tag="PKK95_11510"
     CDS             complement(701..2095)
                     /gene="der"
                     /locus_tag="PKK95_11510"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOC18890.1"
                     /translation="MGRRPSRAVVLVGRPNVGKSTLFNRITSSRRAIVTAVPGTTRDL
                     ITQTAVWRGVTFELTDTGGMYGASQDPLHEMVFAAGLRAIGSADVLVLVVDGREGRTP
                     GDDEIASALRGTGAPVVIAINKIDDRRAQQAALEFYRLGFECVVEVSAEHGQGIGDLL
                     DEIVARLPAGSPAAEPSPGGAEEPEEAGELEAGAPDEVRVAIVGRPNVGKSSLVNRLM
                     RRELVLVSEVPGTTRDAVDALLRWNRRQYRIVDTAGIRRPGRVARSGQVESLSVIIAR
                     RSVEAADVAVLVIDAVEGPAEQDGAIAGEAERAGCGVVIAANKWDLVKGRGQGFAETF
                     DAEVRRRLKFLEYAPLVHISALTGERTPRLLEVVDKVAAARRRRVTTGELNRFVERVA
                     AEHPPASPGRKAVRILYAAQVGVAPPTFVFFTNVATTFHFSYERFLVNRLREEFGFTG
                     TPIRIQVRSRRGEG"
     gene            2167..2436
                     /locus_tag="PKK95_11515"
     CDS             2167..2436
                     /locus_tag="PKK95_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOC18891.1"
                     /translation="MIKVDIVNEVSKAADITKVKAELAVDAVFDAMRVSMQRGDRIEL
                     RGFGVFQVKPRKRGIGRNPRTGKEVRIPPGRTIRFKPGKDLQNIG"
     gene            2575..3510
                     /locus_tag="PKK95_11520"
     CDS             2575..3510
                     /locus_tag="PKK95_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOC18892.1"
                     /translation="MERAAGYPPGLVPGTFPRDRYWLHAALLLLTFGTTTLVGAGHYA
                     AYLMDFKEGPLQPTWGLLVGGLWYSLTIMAILGAHEMGHYLACRYYRINATLPFFIPV
                     PPPVLTGTLGAFIKIRQPVRSKRVLFDIGVAGPLAGFVFAVPALVIGLSLSRVAALPP
                     DFSGLSLGEPLLFKGAAWIMWGTIPEGYSLNLHPMGFAAWFGLLATALNLFPIGQLDG
                     GHISYAVLGRRSSFVTLGAVSVAVLLTFVSMSWLVWTLMLVAMLFFFGPHHPPTVDEH
                     VPLDRARLALAAVALLVFVLSFTPAPIEVLDLVQR"
     gene            complement(3541..4782)
                     /locus_tag="PKK95_11525"
     CDS             complement(3541..4782)
                     /locus_tag="PKK95_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC18893.1"
                     /translation="MTRYTSVIGAALLALAIAAGLRAAQPQVTAIRGGRVVPVNGPAI
                     ENGTVIIADGKITAVGAKAAVPKGASVIDAKGGWVLPGLVDAHTSIGLAGERRDGPPD
                     ELSDANTPQLLVLDGFNPYDRGVRRLVMAGITTALVTPGRANVIAGQPAVVRLAPGSP
                     DGVALLSPAGLKFSLGEGPKKAFGEKSRLPGTRMGAAFVVRKALLDAAEYSRKWKEHE
                     AGGAKAPAPARDLALEPLARLLDGRLTAFIETYRADDIITALRLVDEFKLKAVLMGCT
                     EGSRVAGEIARRKIPVIVGPMGVGPKRVETEEVGLENAARLAQAGVVVAIDSEDEMGI
                     GAPEELPLAAALAVKGGLAPDVALRGITLTAAQLIGVGDRVGSLEPGKDADLTIFTGD
                     PLYYRSRVRTVIAAGRVVYGS"
     gene            complement(4791..>5984)
                     /locus_tag="PKK95_11530"
     CDS             complement(4791..>5984)
                     /locus_tag="PKK95_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC18894.1"
                     /translation="PPAVAQPTETIAFRSARLLTAAGPEIADGVLVIREGKILAVGPA
                     GTVQIPAGARVVDAAGKTITPGLVDAHSHIGLGASGGDTEDNEGSDPVTPQLRVIDSI
                     HPEGMGPDRNQFRSAVAEGVTAVVARPGSGNVIGGQSAALKLRGGTVDEMVLRFPADM
                     KMALGRKMGYASKGQMPTTKMGAAFLVRKALAEAADYKLALERYEKERAAKPDAAPPP
                     RDLAKEAMLKVLNREIPVHIHVGTADDIMTAVRLAREHDFVRLSLVHAEEAWKVAGQL
                     AANHVTVVVGPVMIAYDDENRAINLAQALHEKGVEVCITTDADVVQQPFLRFQAAVAV
                     KHGMDPAEALKAITINPARLAGVGDRIGSLEPGKDADLAIFDGDPFDIQTRVVEVYID
                     GRRAR"
BASE COUNT          813 a         2176 c         2015 g          980 t
ORIGIN      
        1 ggatttgaac ctccgacccc ctgaacccca ttcaggtgcg ctaccagact gcgctacgcc
       61 ccgaccgtcc gtgtattgta ccccagcccg tgcttggctg cacgcgtgca gtcaccgcct
      121 cgagccgtga gccctgagcc tacttcctcc cccgcttccg cttcgttcct cctggcatcg
      181 ccgcggccgg cgggggcgtc gtcgtctcgg cctcggcgtc gagcagcggc agcgccggtt
      241 cgtcatcctc gcgccgcaac gggaccgacg ctcttgccgg cggccaggtg ctggttgagg
      301 gaggtggcgg cgtcgcctcg cgctcgggtc ccaggagctg cagcagcgac ttcaactcct
      361 gcttcaccct gcccacgcgg gccttcacct cctcgggcag cgggaagtcg ggctcgggtt
      421 cgggtggcgg ctccccttcc aggcggcggc gggctcccgc gagcgtcagg ccctcggaga
      481 agaccagctg cttgatgcgc aggatctgct cgacgtcgcc gcgccggtac acgcgcggcg
      541 cgcccgcccg cggcaccact cccaggttcg ggaactcctt ctcccacgaa cgcagcacgt
      601 aaggcggaat ctgcacgagg tcgcacacct cggccgcctt gaaggccggc ttgtcgggga
      661 tgtcgatcag ggagtcggcc acgtcggacg cgagtatacc ttacccctcc ccgcggcgcg
      721 acctgacctg gatgcgtatg ggcgtgcccg tgaagccgaa ctcctcgcgc agccggttga
      781 ccaggaagcg ctcgtacgag aagtggaagg tggtggccac gttggtgaag aacacgaagg
      841 tcggcggcgc caccccgacc tgcgccgcgt agaggatgcg caccgccttg cggcccgggc
      901 tggccggcgg atgctcggcc gcgacgcgct cgacgaaccg gttcagctcg ccggtcgtca
      961 cccgccggcg tctcgcggcg gcaaccttgt cgaccacctc gagcagccgc ggcgtgcgct
     1021 cgcccgtcag cgccgagatg tggacgagcg gcgcgtattc caggaacttc aggcggcgtc
     1081 ggacctcggc gtcgaacgtc tcggcgaagc cctggccgcg gcccttcacg aggtcccact
     1141 tgttggcggc gatcacgacc ccgcagccgg cacgctcggc ctcgcccgca atcgcaccat
     1201 cctgctcggc cggcccctcg acggcgtcga tgacgagcac cgcgacgtcg gctgcttcca
     1261 ccgagcggcg cgcgatgatg acgctcagcg actcgacctg ccctgagcgc gctacccgcc
     1321 ctgggcggcg gatgcccgcc gtgtccacga tccggtactg ccgcctgttc cagcggagca
     1381 acgcgtccac ggcatcgcgc gtcgttcccg gcacctcgct gacgagcacc agctccctgc
     1441 gcatcaagcg gttgacgagc gacgacttcc ctacgttggg ccgcccgacg atggcgacgc
     1501 gcacctcgtc cggagcgcca gcctcgagct cgcccgcctc ctccggctcc tccgccccgc
     1561 ccggcgacgg ttcggccgcg ggcgatccgg caggcaggcg cgccacgatc tcgtcgagca
     1621 ggtcgccgat accctggccg tgctcggccg agacttccac cacgcactcg aagccgaggc
     1681 ggtagaactc gagcgcggcc tgctgcgccc ggcggtcgtc gatcttgttg atcgcgatga
     1741 cgaccggcgc ccccgtccct cgcagcgccg acgcgatctc gtcgtcgccc ggagtgcgcc
     1801 cctcgcggcc gtccacgacc aggaccagga cgtcggccga accgatggct cggaggccgg
     1861 cggcgaacac catttcgtgc agcgggtcct ggctggcgcc gtacatgccg ccggtgtcgg
     1921 tcagctcgaa ggtgacccct cgccacaccg ccgtctgggt gatcaggtcg cgggtcgtcc
     1981 ccggaacggc ggtcacgatc gcccgcctgg atgaggtaat ccggttgaac agggtcgact
     2041 tgccgacgtt gggccggccg accagcacga cggcccgcga gggccgccta cccacgtgac
     2101 acccgccctt gacaggctaa gtggctctct ctatgatagt tagcagcttc cagggagcgc
     2161 ctcagcatga tcaaggtcga catcgtcaac gaggtctcga aagccgcgga cattaccaag
     2221 gtcaaggccg agctggcggt ggacgccgtg ttcgacgcga tgcgggtctc gatgcagcgc
     2281 ggcgatcgca tcgaactgcg cgggttcggg gtgttccagg tgaagccgcg caagcggggc
     2341 atcggccgca acccacgtac cggcaaagaa gtgcggatcc cgcccgggcg gacgatcagg
     2401 ttcaagccgg gcaaggatct gcagaacatc ggctgacacg ccttggcgcc ctttcccgac
     2461 gaccgccccg cccccggacc ggacgaccac catccggcct ctccggggct ggttcccgac
     2521 gagccgccac cctttcgcct gggcagcgtc agtgtagcaa ggatcccgct gcgcgtggaa
     2581 cgagcggccg gctacccgcc cggcctggtt cccggaacgt ttccacgcga ccgctactgg
     2641 ctgcacgccg cgctgctgct gttgaccttc gggacgacca cgctggtcgg cgccgggcac
     2701 tatgcggcgt accttatgga cttcaaggag ggacccctcc agccgacttg ggggctcctg
     2761 gtgggaggcc tctggtacag cctgacgatc atggccatcc tcggggccca cgagatgggg
     2821 cactacctcg cgtgccggta ttaccggatc aacgcgaccc tccccttctt catccccgtc
     2881 ccgccgccgg tgctgaccgg gacgctgggg gcgttcatca agatccggca gccggtccgt
     2941 tcgaagcggg tgttgttcga catcggcgtc gccggtccgc tcgcgggctt cgtgttcgca
     3001 gtgccggcgc tggtgatcgg gctgtcgctc tcccgcgtgg ccgcgctgcc gcccgacttc
     3061 tcggggctgt cgctcggcga gccgctcctc ttcaagggtg cggcgtggat catgtgggga
     3121 acgatcccgg aggggtattc gctcaacctc cacccgatgg gctttgccgc gtggttcggc
     3181 ctgctggcga cggcgctgaa cctgttcccg atcggccagc tcgacggcgg gcacatctcc
     3241 tacgccgtgc tggggcggcg atcgtcgttc gtcaccctcg gcgcggtcag cgtcgccgtc
     3301 ctcctgacgt tcgtctcgat gagctggctc gtctggacgc tgatgctggt ggcgatgctc
     3361 ttcttcttcg ggccccacca ccctccgacg gtggacgagc acgtgcccct cgaccgggcg
     3421 cgcctggcgc ttgccgccgt cgccctgctg gtcttcgtgc tcagcttcac cccggcgccg
     3481 atcgaggtgc tggatctcgt gcagaggtaa cgatgcacgg ggcgaccaca agggtcgccc
     3541 ctacgaaccg taaaccacgc ggccggcggc gatcaccgtc cgaacgcgcg atctgtagta
     3601 cagcgggtcg ccggtgaaga tcgtgaggtc ggcgtccttg ccgggttcca ggctgcccac
     3661 ccgatcgccg accccgatca actgcgccgc ggtcagcgtg atgccccgaa gcgcgacgtc
     3721 gggcgcaagc ccgcccttga cggcgagggc ggcggcgagc ggcagctcct cgggggcgcc
     3781 gatccccatc tcgtcctcgc tgtctatcgc gacaaccacg cccgcctgcg cgagcctggc
     3841 ggcgttctcg aggcctacct cctcggtttc gacccgcttc ggtccaacgc ccatgggccc
     3901 gacgatgacg gggattttgc ggcgggcaat ctcgccggcg acacgcgatc cctcggtgca
     3961 gcccatgagc accgccttga gcttgaactc gtccaccagc cgtagtgccg tgatgatgtc
     4021 gtcggcgcgg taggtctcga tgaacgccgt cagccggccg tccagcagcc gggcgagcgg
     4081 ctcgagggca aggtctctcg ccggggccgg cgccttcgcg cctccggcct cgtgctcttt
     4141 ccacttcctc gagtactcgg ccgcgtcgag cagcgccttg cggacgacga aggccgcccc
     4201 catacgcgtg cccggcagcc ggctcttctc gccgaacgcc ttcttcggcc cctcgccgag
     4261 cgagaacttc aggcccgccg gtgacagcaa ggcgaccccg tcgggcgacc cgggagcgag
     4321 gcgcacgacg gcgggctgcc cggcgatgac gttcgcgcgg ccgggcgtca ccagcgcggt
     4381 ggtgatgccc gccatgacga gccggcggac gccccggtcg tagggattga agccgtcgag
     4441 caccagcagc tgcggcgtgt tggcgtccga cagctcgtcg ggcggcccgt cgcggcgctc
     4501 gcccgccaga ccgatgctgg tgtgcgcgtc gacgaggccg ggaagcaccc agcctccctt
     4561 cgcgtcgatg acggaggcgc ccttcggtac ggccgccttc gccccgacgg ccgtgatctt
     4621 cccatccgca ataatcacgg tgccgttctc gatggcgggg ccgttcacgg gtaccacccg
     4681 gccgccgcgg atggcagtca cctggggctg ggcggcgcga aggccggcag cgatcgccag
     4741 ggcaagcagg gccgctccaa tcacgctggt gtaccgtgtc atgccgtacc tcatcgcgcg
     4801 cgccggccgt cgatgtacac ctcgacgacg cgggtctgga tgtcgaaggg gtcgccatcg
     4861 aagatggcga gatcggcgtc cttgcccggt tcgaggctgc cgatccggtc cccgacgccc
     4921 gccaggcgtg ccgggttgat cgtgatcgcc ttcagcgcct cggccgggtc catgccgtgc
     4981 ttgacggcga cggcggcctg gaatcgcagg aaaggctgct gcaccacgtc ggcatcggtc
     5041 gtgatgcaca cctcgacccc cttttcgtgc agcgcctgcg ccaggttgat cgcccggttc
     5101 tcgtcgtcgt aggcgatcat caccggcccg acgacaacgg tgacgtggtt ggctgcaagc
     5161 tggccggcca ccttccatgc ttcttcggca tgcacgagcg agaggcgcac gaagtcgtgc
     5221 tcgcgggcca gccggacggc ggtcatgatg tcgtcggccg tcccgacgtg gatgtgcacc
     5281 gggatctccc ggttcaacac cttcagcatc gcctccttcg ccaggtcgcg aggaggcggc
     5341 gcggcgtcgg gcttggcggc gcgctccttc tcgtaccgct cgagggcgag cttgtagtcg
     5401 gccgcctcgg ccagcgcctt ccgcaccagg aacgcggcgc ccatcttggt cgtgggcatc
     5461 tgccccttgc tcgcgtagcc catcttgcgg ccgagcgcca tcttcatgtc ggcagggaag
     5521 cgaagcacca tctcgtccac ggtgccgccg cgcagcttca gcgctgccga ctgccccccg
     5581 atcacgttgc cgctgccggg ccgtgccacg acggccgtga caccctcggc gacggccgac
     5641 cggaactggt tgcggtccgg gcccatcccc tcggggtgga tcgagtcgat caccctgagc
     5701 tgcggggtca ccggatcgct tccctcgttg tcctcggtgt cgccgccgct cgccccgagg
     5761 ccgatgtgcg agtgggcgtc cacgaggccg ggggtgatcg tctttccggc ggcgtcgacg
     5821 actcgggctc ccgcggggat ctgaaccgtt ccggccggcc cgacggcgag gatctttcct
     5881 tccctgatga cgaggacgcc gtcggcgatt tcgggcccgg cggcggtgag aaggcgggca
     5941 ctgcgaaacg cgattgtctc tgtgggctgc gcgacggcag gggg
//
LOCUS       DAOPQX010000216         5982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14521_length_5982_cov_3.918677, whole genome shotgun sequence.
ACCESSION   DAOPQX010000216 DAOPQX010000000
VERSION     DAOPQX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14521_length_5982_cov_3.918677"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="tyrS"
                     /locus_tag="PKK95_11535"
     CDS             complement(<1..533)
                     /gene="tyrS"
                     /locus_tag="PKK95_11535"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOC18895.1"
                     /translation="MTGNVWDEFEWRGMIYEATEGAREALARPLTCYIGFDPTASSLH
                     VGSLLPLMALARLQRFGHSPIAIAGGGTGMIGDPSGKTQERQLLDLDRIEENVAGIRR
                     QLERFLDFDARPNPARIVNNADWLAKLDLMTFLRDTGKHFTVNYMLAKESVRRRLESE
                     DGISYTEFSYLMLQSYD"
     gene            complement(622..1971)
                     /locus_tag="PKK95_11540"
     CDS             complement(622..1971)
                     /locus_tag="PKK95_11540"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOC18896.1"
                     /translation="MKGVLTPGPTGILEFGPFQLFPDGRFLRDGRRVPLSPKEWGVLR
                     VLAGASGALVTKESLLSQVWPGETVSDASLVRAIYGLRRRLGLTDAGDEYIATSYGRG
                     YWLNASVHAAPGLGAGGSRSDGTHGVHTGVSPRVLEACQEARYHLRQRNVKRIYEAIR
                     LYRLATEWGPSYAPGYLGLVKCYLTLVGSSVVPRAEGLPPALAALEVAGSLDPDNPEV
                     LAVRAILLSAFEWRFAEAEPLFERARHSPREGPDVGMFHARHLLLAGDAASSASLLDQ
                     YAERDPLSPPLLALLGYSHFCAGHPGEALAAARQALALDRRSTEALVYFAVIAMHAGR
                     RQEASAAGYRAYRQTRGLAPVAASWAYLCACDGRQEEARRLMREAAATPSFLAPAVMA
                     VTAWTLADPVEAAAWTRRAVAMRCVWLPVACRDPRFTPALGHPDVAAALAGTPFVCR"
     gene            2060..2878
                     /locus_tag="PKK95_11545"
     CDS             2060..2878
                     /locus_tag="PKK95_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC18897.1"
                     /translation="MRVTRRRALQTLVAAGTGLAGLGGYGLLVEPHRLSVTRASVRVS
                     RLPPALDGLRLGLVADLHYGRPTGDGMLASITETLAAEKPDLVLLVGDFVTGSERSAV
                     APCAARLTGIDARYGVFAALGNHDPEAEVKWAFERQGIGVLRDGHTRLAIRGEPVSLG
                     GLRYWSRKAADLQRAFKGARGFSILLAHDPRRLDLAAAAGIPLVLSGHTHGGQVVLPG
                     IGAPAAARYPVVHGIGRRGPTTLFVTRGIGTVVLPVRFNCPPELAVLTLQPAVG"
     gene            complement(2894..5071)
                     /locus_tag="PKK95_11550"
     CDS             complement(2894..5071)
                     /locus_tag="PKK95_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC18898.1"
                     /translation="MKTSAIVSAVALFALFGAVVAGTRQQASTSMLQHTHARLQYPEA
                     KKVDVVDEYFGHPVADPYRWLEDPDASDTRRWIDAENALTGRYLDSIPDRARIKERLT
                     ALWNYPRYSAPSREGSYYVFTRNDGLQPQAVVYKAASPGAPPEVLLDPNTFSEDGTVA
                     LGGLSFSEDGRYVAYALASSGSDWLEWRVRDVAAGADLADRLKWSKFSGAAWRKDGSG
                     FYYGRYDEPGPGAALQAVNKNQKLFFHAVGTPQKDDRLVYERPDQPDWGFSAEVTDDG
                     RFLVIVQTEGTDNRNRVFLQDLSRPGTAVEPFLDAFDAAYDVVGNDGETFYVRTNKGA
                     PRYRLVAIDRRKTVPESWRTLIPEAANRAVLESVAMVSDQFVARWLADAHSRLAVHGL
                     DGSVAREIALPEPLGTVAELTGRRAHREAFFAFTSFLSPTSIYRYDFEASATTTLWKP
                     EVPFDASGYETVQVFYQSKDGTRVPMFLTMKKGLEKNGQHPTYLYGYGGFDIPLTPGY
                     SPAMAAWLEMGGIYAVANLRGGSEYGQEWYDAGRLANKQNVFDDFIAAAEYLIREKYT
                     STPRLAIAGASNGGLLVGACMTQRPGLFGAALPAVGVMDMLRYHKFTIGWAWKSDYGD
                     PDTREGFEMNLEYSPLHNLRSGTSYPATLVTTADHDDRVVPAHSFKFAARLQEVQSGP
                     APVLIRIETKAGHGAGKPTDKQIEERADVFAFLVRELGMELPR"
     gene            complement(5081..5692)
                     /gene="purN"
                     /locus_tag="PKK95_11555"
     CDS             complement(5081..5692)
                     /gene="purN"
                     /locus_tag="PKK95_11555"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOC18899.1"
                     /translation="MNRTLGVLISGRGSNLQAIVDAIREGRLDARIAVVISNRADAYG
                     LERARAAGIEALCISHRAFPTREAFEAALVAELRARDVGLVCLAGFMRLLGPAFVEAF
                     PNAILNIHPSLLPSFPGLDAQRQAVEHGVKVSGVTVHLVDCQLDAGPIVVQRAVPVRD
                     DDTAETLANRILAVEHQAYPEAIRIVLDRRWRIEGRRFLSEPQ"
     gene            complement(5739..>5982)
                     /locus_tag="PKK95_11560"
     CDS             complement(5739..>5982)
                     /locus_tag="PKK95_11560"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOC18900.1"
                     /translation="IDKGAWEVPPLFTFLGRAGGVPEDDMYRTFNMGIGLIVGCAAAD
                     VDRVLARLGPLPAPGPRVIGELVAGPREVRYRGTRS"
BASE COUNT          876 a         2069 c         2173 g          864 t
ORIGIN      
        1 taatcgtagg actgcagcat gaggtagctg aactcggtgt aggagatccc gtcctccgac
       61 tcgaggcgcc ggcgcaccga ctccttcgcg agcatgtagt tgaccgtgaa gtgcttgccc
      121 gtgtccctca ggaacgtcat caagtcgagc ttcgcgagcc agtcggcgtt gttgacgatg
      181 cgggccgggt tggggcgggc gtcgaagtcg aggaagcgtt cgagctgacg gcggatgccg
      241 gcgacgttct cctcgatccg atcgaggtcg agcagctgcc gctcctgcgt cttgccgctc
      301 gggtcgccga tcatcccggt gccgccgccg gcgatcgcga ttgggctgtg accgaatcgc
      361 tggaggcggg cgagcgccat caggggcagc agcgagccga cgtgcaggct cgacgcggtc
      421 gggtcgaagc cgatatagca ggtcagcggc cgcgcgagcg cctcgcgggc gccctcggtt
      481 gcttcgtaga tcatcccccg ccactcgaac tcgtcccaca cgttgccagt catcgcgatg
      541 cctccacgag aactccctac tatagcaagg cggcgacggg cgggcggggg ggagagagtt
      601 gtgtggcgag gggtccgcct gtcagcggca gacgaacggc gtgccggcca gggccgcggc
      661 cacgtcgggg tggccgaggg ccggcgtgaa acgcgggtcg cggcaggcca ccggcagcca
      721 gacgcagcgc atggcgacgg cgcgccgcgt ccaggccgcc gcctccacgg ggtcggccag
      781 cgtccaggcc gtcacggcca tgaccgcggg cgcgaggaac gagggcgtcg ccgccgcctc
      841 ccgcatcagg cgccgcgcct cctcctggcg cccatcgcac gcgcacaggt aggcccacga
      901 cgcggcgacc ggcgcaaggc ccctggtctg acggtaggcg cggtaccctg ccgcggaggc
      961 ctcctggcgg cggccggcgt gcatcgcgat caccgcgaag tagacgagcg cctcggtgct
     1021 ccggcggtcg agcgccaagg cctggcgggc cgccgccagc gcctcgccgg gatggccggc
     1081 gcagaagtgc gaatagccga gcagcgcgag cagcgggggt gacagcggat cgcgctcggc
     1141 gtactggtcg aggaggctgg cggaggacgc ggcgtcgccg gcgagcagca ggtgacgcgc
     1201 gtggaacatc ccgacatcgg ggccctcgcg cggcgagtgc cgggcacgtt cgaagagggg
     1261 ctcggcttcg gcgaacctcc actcgaacgc actcaggaga atcgcgcgga ccgccagcac
     1321 ctcggggttg tcggggtcga gggagccggc gacctcgagc gccgccaggg ccggcggcag
     1381 cccttcggcc cggggcacca cgctcgaccc gaccagcgtc aggtagcact tgaccaggcc
     1441 gaggtacccg ggcgcgtacg acgggcccca ctcggtcgcg aggcggtaga gcctgatcgc
     1501 ctcgtagatc cgcttcacgt ttcgctggcg gaggtggtag cgggcctcct ggcaggcttc
     1561 gagcacgcgc gggctcacgc cggtgtgcac tccgtgggtg ccgtcggatc tcgagccgcc
     1621 cgcaccgagg cctggcgccg cgtgaacgga tgcgttgagc cagtagcccc gcccgtacga
     1681 cgtggcgatg tactcgtcgc cggcatcggt cagcccgagg cgccgccgga ggccgtagat
     1741 cgcgcgtacg aggctcgcat ccgagaccgt ctcgcccggc cagacctgcg acagcagcga
     1801 ctccttcgtc acgagcgcac cagaggcgcc cgcgagcacc cgcagcacac cccactcctt
     1861 ggggctgagc gggacgcgcc ggccgtcgcg caggaaccgg ccatcgggaa agagctgaaa
     1921 cggtccgaac tccaggatcc cggtcggtcc aggggtcaag actcccttca cgcacaaatt
     1981 gtcacccaac tccgtccgcg agtccatgcg caagtccact tgaagcggcg gccgcgaggc
     2041 cccgcgtata atcgcctcca tgcgggtcac ccgccgtcgc gcactccaga ctctcgtcgc
     2101 ggcgggcacc ggcctcgcgg ggcttggagg atacggcctg ctggtcgaac cgcaccggct
     2161 gagcgtcacg cgtgcgagcg tgcgggtctc gcgcctgccg cccgcgctcg acggcctccg
     2221 gctgggactg gtcgccgacc tgcactacgg caggccgacc ggggatggca tgctggcgtc
     2281 gatcacggag accctggccg ccgagaagcc ggacctcgtg ctgctggtgg gggacttcgt
     2341 gaccgggtcc gagcgctcgg ccgtggcgcc gtgcgcggcc cggctgaccg ggatcgacgc
     2401 gcgatacggc gtgtttgcgg cgttggggaa ccacgatccc gaggccgagg tgaagtgggc
     2461 gttcgagcgg caggggatcg gcgtgctgcg cgacggtcac acccgcctgg ccatccgggg
     2521 tgagcccgtg tcgcttggag ggctgcgcta ctggagccgc aaggccgccg acctgcagcg
     2581 cgcgttcaag ggggcgaggg gattctcgat cctgctcgcg cacgatccga ggcgcctcga
     2641 cctggcggcc gccgccggga ttcccctggt cctctcgggg cacacgcacg gcgggcaggt
     2701 ggtgctgccc ggcatcggcg cccccgccgc ggcgcgctac ccggtggtgc acggcatcgg
     2761 gcgccgtggg ccgaccacgc tcttcgtcac gcggggcatc gggacggtcg tcctgcccgt
     2821 ccggttcaac tgcccgcccg agctggccgt cctgacgctc caaccggcgg tcggctgagc
     2881 agccgccggg ccgctatcgc ggcagctcca tccccagctc cctcacgaga aacgcgaaca
     2941 cgtcggcccg ctcctcgatc tgcttgtcgg tgggcttgcc cgccccgtgc ccggccttgg
     3001 tctcgatgcg gatcagaacg ggcgcgggcc ccgactgcac ctcctgcagc cgcgccgcga
     3061 acttgaagct gtgcgcaggc accacgcggt cgtcatggtc ggcggtggtg acgagcgtcg
     3121 ccgggtagct cgtgccggac cgcaggttgt gcagcggcga gtactcgagg ttcatctcga
     3181 acccctcgcg agtgtcgggg tcgccgtagt cggacttcca cgcccagccg atcgtgaact
     3241 tgtggtacct cagcatgtcc atgacgccga cggccggcag cgccgccccg aacaggccgg
     3301 gccgctgcgt catacaagcg cccacgagca gaccgccgtt gctggcacca gcgatggcaa
     3361 ggcggggcgt ggaggtgtac ttctcgcgga tcaggtactc ggcggcggcg atgaaatcgt
     3421 cgaagacgtt ctgcttgttc gcgagccggc cggcgtcgta ccactcctgg ccgtactcgg
     3481 atccgccccg aaggttcgcc accgcgtaga tgccgcccat ttcgagccac gccgccatcg
     3541 ccggcgagta gccgggtgtc agcgggatgt cgaagccgcc gtagccgtac aggtaggtcg
     3601 ggtgctgccc gttcttctcc agccccttct tcatcgtcag gaacatgggg acgcgagtgc
     3661 cgtccttcga ctggtagaag acctggaccg tctcgtagcc ggacgcgtcg aaaggcacct
     3721 cgggcttcca gagcgtggtc gtggcagagg cctcgaagtc gtagcggtag atcgaggtcg
     3781 gcgacaggaa cgacgtgaac gcgaagaacg cctcgcggtg ggcgcgccgg cccgtcagct
     3841 cggccacggt gcccagcggc tcgggcagcg cgatctcgcg ggcaaccgag ccgtcgagcc
     3901 catggacggc gaggcggctg tgggcgtcgg cgagccagcg ggccacgaac tggtcgctca
     3961 ccatcgcgac cgactcgagg acggcgcgat tggccgcctc ggggatgagg gtccgccacg
     4021 actcgggcac cgtcttgcgg cgatcgatcg cgaccagccg gtagcgcggg gctcccttgt
     4081 tggtgcggac gtagaacgtc tcgccgtcgt tgcccaccac gtcgtaggcg gcgtcgaacg
     4141 cgtcgaggaa cggctcgacc gcggtgccgg gacgcgacag gtcctggagg aacacccggt
     4201 tacggttgtc ggttccctcc gtctgcacga tcaccaggaa gcggccgtcg tcggtcacct
     4261 cggccgagaa gccccagtcc ggctgatcgg gacgctcgta gacgaggcga tcgtccttct
     4321 gcggcgtccc gacggcatgg aagaaaagct tctggttctt gttcaccgcc tggagggcgg
     4381 cgcccggccc gggctcgtcg tagcggccgt aatagaagcc cgacccgtcc ttcctccagg
     4441 cggcgccgct gaacttcgac cacttgagcc ggtcggccag gtcggcgccg gcggccacgt
     4501 cgcgcactcg ccactcgagc cagtccgatc cactcgacgc cagagcgtac gccacatagc
     4561 ggccgtcctc ggagaacgag agccccccga gcgcgacggt gccgtcctcc gagaaggtat
     4621 tcgggtcgag cagcacctct ggcggagcgc cgggcgaagc cgccttgtag acgaccgcct
     4681 gcggctgcag cccgtcgttc cgggtgaaga cgtagtaaga cccctcccgg gacggagcgc
     4741 tgtagcgcgg gtagttccag agggccgtga ggcgctcctt gatgcgcgcc cggtcgggga
     4801 tcgagtcgag gtaacgcccg gtcagcgcgt tctcggcgtc gatccaccgc ctggtgtcgg
     4861 acgcgtcggg gtcttcgagc cagcggtacg ggtcggctac cgggtggccg aagtactcgt
     4921 ccaccacgtc gaccttcttc gcttcggggt actgcagccg ggcgtgggtg tgctggagca
     4981 tggaggtgct ggcctgctgc cgggtgccgg cgacgaccgc gccgaacagg gcgaacaggg
     5041 cgacggcgga cacgatggcg gacgtcttca ttggtgcctg ctactggggc tcggagagga
     5101 agcggcgtcc ctcgatccgc caccggcggt cgaggacgat ccggatggcc tcgggatacg
     5161 cctggtgctc gacggccagg atgcgattgg ccagcgtctc ggcggtgtcg tcgtctcgaa
     5221 cggggacggc gcgctgcacg acgatcggcc ccgcgtcgag ctggcagtcg accaggtgca
     5281 cggtcacccc gctcaccttc acgccgtgct cgaccgcctg gcgctgcgcg tcgagaccgg
     5341 ggaaggacgg cagcagcgag gggtggatgt tgaggatcgc gttggggaac gcctcgacga
     5401 acgcggggcc gagcagccgc atgaaccccg cgaggcacac aagcccgacg tcccgcgcgc
     5461 gcagctcggc gacgagtgcc gcctcgaagg cctcccgcgt ggggaaggcg cggtgactga
     5521 tgcacagcgc ctcgatcccc gccgcccggg cgcgctccag gccgtaggcg tcggcgcggt
     5581 tcgagatgac gaccgcgatg cgcgcgtcga ggcgcccctc gcgaatggcg tcgacgatcg
     5641 cctgcaggtt gcttccgcgg ccggagatca gcacacccag cgttcggttc atcgtggtgt
     5701 tgccgcgtcg gggggctgcc tgtggccgga gcgcggtctc aagaccgggt tccccggtac
     5761 cgcacctcgc gggggccggc gaccagctcg ccgatcactc gcggcccggg cgcgggaagc
     5821 ggcccgagcc gggcgagcac gcgatcgaca tcggccgccg cgcaccccac gatcagcccg
     5881 atccccatgt tgaaggtgcg gtacatgtcg tcctcgggca ctccgccggc ccttccgagg
     5941 aaggtgaaca ggggcggcac ctcccacgcc cccttgtcga tc
//
LOCUS       DAOPQX010000217         5918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14707_length_5918_cov_3.749616, whole genome shotgun sequence.
ACCESSION   DAOPQX010000217 DAOPQX010000000
VERSION     DAOPQX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14707_length_5918_cov_3.749616"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKK95_11565"
     CDS             <1..367
                     /locus_tag="PKK95_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOC18901.1"
                     /translation="AGLSMGGMQTYHITLNHLDLFSHIGGFSGAAGGMSLGRDLDPKT
                     DYNGVFADPAAFAKKVRLLWLGVGTAEPERMREGIRNLHASLTSAGIAHVYQESEGTD
                     HEWQTWRRNLNDFAARLFR"
     gene            387..1535
                     /locus_tag="PKK95_11570"
     CDS             387..1535
                     /locus_tag="PKK95_11570"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOC18902.1"
                     /translation="MKNSSILAGVCVVVTVVTWTAGPLAAQSHTRVKTPAAPGEQAPP
                     ADPGQRNFGGPIVVPGPGQKPPAPPRFPQPKFGTVPLDQLAMSDPFVFADEKTRTYYL
                     IGSGGRLYKSPDLKMWTGPYSVIDLKGTWMDGRFVAAPEIHRIGGKYYLAGTWNDHGH
                     LIENIPRRYNVPTNQTQLLVADSPDGPFKPLVQEFDFCLGPGDWDIIDGTLYEEDGIT
                     YMVFVHEWTQLIDGTMDYMPLSKDLTRRTAEPTTIFRASEAAWSKEMNSIGEATFGRK
                     MPGWVTDGPQLFRTRTGRLGMLWSSWGENRYAQGVAYSASGSIKGPWVQEKDALMGNN
                     SGHGMLFRTFDGRLLFIVHHAEGNGPRKPQIWNADDSGDTLVLKDRYF"
     gene            complement(1532..2989)
                     /locus_tag="PKK95_11575"
     CDS             complement(1532..2989)
                     /locus_tag="PKK95_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOC18903.1"
                     /translation="MGVQLDRYHVLDNLLEGCQVLSFDWRYLYVNEAVARHARIPRET
                     LLGRTMTELYPGIDRSEVFAVLRRCMEERTTGHFENEFTYPNGSRAWFELRIEPVPEG
                     LFVLSLDITDRKHSEQSMRRQIERLRSLRAIDVAILGSTDVRVALQTVLEETRKRLEV
                     DMAAIFLPEGPTGLAVVACIGFECHRACRTRLGEGAVGCAALRRTTVVVPDAAAAAAR
                     RDVPAAILEAGIEGFCATPLIARGHLVGVLAVARRGPLRPDEDWIGFLESLAGQAAMA
                     IDAGKSYEDLERAHVELALAYDTTIEGWAAALELRDKDTANHTLRVAELTMRLCRAAG
                     MGEGELVHVRRGALLHDIGKMAIPDSILLKEGRLTDEEVSVMQRHPAYAFELLSRIEY
                     LRPALDIPYAHHERWDGSGYPRGLKGEDIPLAARLFAVVDVWDALRSPRPYHAGASEE
                     SAREQLRRLAGTHLDPGAVDLFLSVLGAGDAEGGG"
     gene            complement(3083..5572)
                     /locus_tag="PKK95_11580"
     CDS             complement(3083..5572)
                     /locus_tag="PKK95_11580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18904.1"
                     /translation="MLPIWLEHLWRDVRYGGRMLARSPGFTAIAVASLAIGIGANCAI
                     FSFADALLLRPLPVARPGEILTLGSTTSIEALGSSGLNSSYRDYVDIRDRSKSFAGVA
                     AFSYVTAGFASRPGEVPSLKIGMLASGNLFSVMGVKPALGRGFGPEEDRVPGRDTVVV
                     LGHALWQQQFGSDPSVIGRRVDFNGRPFTVIGVAPPGFTGLDQYVRADFFVPLMTSPL
                     MLNDPKAASLEARDARNLRLKGRLAPGASQASARAELTAIGADLERAYPDTNKNRQWA
                     VHTELQARVAQSPPDAMLVAMLATLAAAVLLVACANVAGLLTSRAPVRAREMAMRLAI
                     GAGRRRLVRQLVIESLLTALAGGVVGLGVGILGLSLFRQIELPTDLPIQVTFRMDERA
                     LLFSLVVSLASAVLCGLLPALKATRSDLTALIKAGDAADPRRRRRWGRTLLVGGQVAV
                     SVVLLAVALFMYRGFSAQLASGPGYRTDHLLLMSFDTSLVRYTPAQSAQFFKEVADRA
                     REVPGVVSVSMTTAVPMVGTNADFQTVVPEGFQFPAGKENATVLAARVDEHFFPTMGL
                     TLLRGRNFRAEDTAGAPMVAIVNEQFARRYWPNDDPIGKRLRVVRDDEAWVEVVGLAK
                     TSKYLFIAEPPTEFVYFPCRQHPRARMVMLARSAGDPAGLANPLREVAHRLDRDMPIY
                     DVRTMADVYRLRATKIFNVIIGAVAGMGLMGLGLAIVGLYGLVSYAASRRTREIGIRM
                     AIGADQAAVLKMVLRHGLVLAVVGLVIGLAASIGVGRLLAAAFPTGMEHQDDLAVLLV
                     IPVVLTVTFLAAYVPARRAARTSPIQALRQE"
     gene            complement(5643..>5918)
                     /locus_tag="PKK95_11585"
     CDS             complement(5643..>5918)
                     /locus_tag="PKK95_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18905.1"
                     /translation="SADVSAHTQTQALLVHHSELAADGAFARPDETIPIEDEPDHEEL
                     CHREQQFFLRAVRDDLDLSDHWQGAYDSLRIVLAADQSRKEGRTIAL"
BASE COUNT          969 a         2083 c         2017 g          849 t
ORIGIN      
        1 ggcgggcctc tcgatgggcg ggatgcagac ctaccacatc acgctcaacc acctcgacct
       61 cttctcccac atcggcgggt tcagcggcgc ggcgggcggc atgtcgctcg gccgcgacct
      121 cgacccgaag accgactaca acggcgtgtt cgccgacccc gcggccttcg ccaagaaggt
      181 gcgcctgctc tggctcggcg tcggcacggc ggagccggag cggatgcgtg aaggcatccg
      241 gaacctgcac gcgtcgctga cttcggccgg cattgcgcac gtctaccagg aatccgaggg
      301 gaccgaccac gagtggcaga cctggcggcg caacctcaac gacttcgccg cgaggctgtt
      361 ccggtaaggg tctcggagaa ttgaagatga agaactcgtc catcctggca ggcgtttgcg
      421 tcgtcgtcac ggtcgtcacc tggactgccg gacccttggc cgcgcagtcg cacacccgcg
      481 tcaagacccc cgcggccccg ggtgaacagg cgcctcccgc cgaccccggg cagaggaact
      541 tcggcgggcc gatcgtagtc cccggccctg gacagaagcc gcccgccccg ccacgctttc
      601 cccagccgaa gttcggaacg gtcccgctcg accagctcgc gatgagcgac ccgttcgtct
      661 tcgccgacga gaagacgcgg acctactacc tgatcggcag cggggggcgt ctctacaaga
      721 gccccgacct gaagatgtgg acgggcccct actcggtcat cgatctgaag ggcacgtgga
      781 tggacggccg cttcgtcgcc gcgcccgaga tccatcgcat cggcggcaag tactacctcg
      841 cgggcacgtg gaacgatcac ggccacttga tcgaaaacat cccgcgccgg tacaacgtgc
      901 cgaccaacca gacccaattg ctggtggccg actccccgga cggaccgttc aagccgctcg
      961 tgcaggagtt cgacttctgc ctcggccccg gcgactggga catcatcgac ggcaccctct
     1021 acgaggagga cgggatcacc tatatggtct tcgtccacga gtggacccag ctcatcgacg
     1081 ggaccatgga ttacatgccc ctgtcgaagg acctcacgcg ccgcaccgct gagccgacga
     1141 caatcttccg cgcgagcgag gccgcgtggt cgaaggagat gaacagcatc ggcgaagcca
     1201 ccttcggcag gaagatgccg gggtgggtca ccgacgggcc acagttgttc cggaccagaa
     1261 ccggccggct cgggatgctc tggtcgagct ggggcgagaa ccgttacgcg cagggagtcg
     1321 cctactcggc gtcgggaagc atcaaggggc cgtgggtcca ggagaaggac gcgctgatgg
     1381 gcaacaactc gggccacggc atgctgttcc ggaccttcga cggacggctg ctcttcatcg
     1441 tccatcacgc cgagggcaac gggccgcgca agccccagat ctggaacgct gacgactcgg
     1501 gggacacgct cgtgctgaag gacaggtact tctaaccccc gccttcggcg tctcccgccc
     1561 cgagaacact caggaacagg tccactgcgc cgggatccag gtgagtgccc gcgagccgcc
     1621 gcagctgctc gcgggcgctc tcctcggacg cgcccgcgtg gtacgggcgg gggctgcgca
     1681 gcgcgtccca cacgtccacc acggcgaaca ggcgtgcggc aagcgggatg tcctcgccct
     1741 tcagcccgcg ggggtagccg ctgccgtccc agcgttcgtg gtgggcgtac gggatgtcga
     1801 gcgccgggcg caggtactcg atccgcgaca gcagctcgaa ggcgtaggcc gggtgccgct
     1861 gcatcaccga gacctcttcg tcggtgagcc tgccttcctt gaggaggatg gagtcgggga
     1921 tggccatctt gccgatgtcg tgcagcaggg cgcctcgcct gacatgcacc agctcgccct
     1981 cgcccattcc cgccgcccgg cagagccgca tggtcagttc cgccacgcgc agcgtgtggt
     2041 tggccgtgtc cttgtcgcgc agctcgagcg cggccgccca cccctcgatg gtggtgtcgt
     2101 aggcgagcgc cagctccacg tgcgcccgct cgaggtcctc gtacgacttg ccggcgtcga
     2161 tcgccatcgc cgcctgcccg gccagcgact ccagaaaccc gatccagtct tcgtccggcc
     2221 gaagaggacc gcggcgcgcg acggcgagca cgccgaccag gtgcccccgg gcgatgagcg
     2281 gcgtcgcaca gaagccctcg atccccgcct cgaggatcgc cgcggggacg tcgcggcgcg
     2341 cggcggcggc cgccgcgtcg ggaaccacga cggtcgtccg gcgaagggcg gcacacccca
     2401 cggctccttc ccccagcctg gttcgacagg cccggtggca ctcgaagccg atgcacgcca
     2461 ccaccgccag gcccgtcggg ccctcgggga ggaagatggc ggccatgtcg acttcgagcc
     2521 gcttccgtgt ttcctccaac acggtctgca gcgcgacgcg gacgtcggtg gagccgagga
     2581 tcgcgacgtc gatggcacgc agcgatcgga ggcgctcgat ctgccggcgc atcgactgct
     2641 ccgagtgctt ccggtcggtg atgtcgagcg agagcacgaa caggccctcg ggcaccggct
     2701 cgatccgcag ctcgaaccag gctctcgacc cgttggggta ggtgaactcg ttctcgaaat
     2761 gcccggtcgt ccgctcctcc atgcaccgcc gcagcaccgc gaacacctcg cttcggtcga
     2821 tgccgggata gagctccgtc atcgtcctgc cgaggagtgt ctcgcgcggg atcctcgcgt
     2881 gcctcgcgac cgcctcgttc acgtacaggt atctccagtc gaaggacagc acctggcagc
     2941 cctcgagcag gttgtcgagc acgtgatacc ggtcgagctg gacgcccatc gattgattat
     3001 gggacgccgg cggcgcgccg gctagaggac tctgctcctg atgagcctgt gattgaagtc
     3061 acggcaggtt gaagacccga ggctactcct gccgcagggc ctggatcggg ctggtgcgtg
     3121 cggcacgccg agccggcacg taggccgcga ggaaggtgac cgtgagcacg accgggatca
     3181 ccagcagcac cgccaggtcg tcctggtgct ccatgcccgt cgggaacgcg gccgccagca
     3241 accgcccgac gccgatgctc gctgcgagcc cgatgaccag ccccacgacc gcgagcacga
     3301 ggccgtgccg gagcaccatc ttgaggacgg cggcctggtc ggcgccgatc gccatccgga
     3361 taccgatctc ccgcgttcgc cggctggccg cgtacgacac tagcccgtag aggccgacaa
     3421 tcgcgaggcc aagacccatg aggcccatgc ccgccaccgc cccgatgatc acgttgaaga
     3481 tcttcgtcgc gcgcagccgg tagacatctg ccatcgtgcg cacgtcgtag atcggcatgt
     3541 cccggtcgag ccggtgcgcg acctctcgca gcgggttggc caggcccgcg ggatcgccgg
     3601 ccgagcgcgc cagcatgacc atcctggccc tggggtgctg ccggcagggg aagtacacga
     3661 actcggtcgg gggctcggcg atgaagagat acttgctggt cttcgcgagc cccaccacct
     3721 ccacccaggc ctcgtcgtcg cgcaccacgc gcaggcgctt cccgatcggg tcgtcgttcg
     3781 gccagtagcg ccgcgcaaac tgctcgttca cgatcgccac catcggcgcg ccggccgtgt
     3841 cctcggcgcg gaagttgcgc ccccgaagga gcgtcagccc catcgtcggg aagaagtgct
     3901 cgtccacgcg ggccgccagc accgtcgcgt tttccttgcc cgcggggaac tggaacccct
     3961 cggggacgac cgtctggaag tcggcgttgg tgcccaccat cgggaccgcc gtcgtcatcg
     4021 agacgctgac gacgccgggc acctcgcgcg cgcggtcggc gacctccttg aagaactgcg
     4081 ccgactgggc cggcgtgtac ctcaccaggc tggtgtcgaa gctcatcaac aagaggtgat
     4141 cggtgcggta gcccggaccc gaggcgagct gggcgctgaa gccgcggtac atgaagagcg
     4201 cgacggcgag cagcaccacc gaaacggcaa cctgccctcc caccaggagc gtacgccccc
     4261 aacggcggcg gcgtcggggg tcggcggcat cgcccgcctt gatcagcgca gtgaggtcgg
     4321 agcgggttgc cttgagtgcc ggcagcaggc cgcacagcac cgcgctggcg agcgagacga
     4381 cgaggctgaa cagcagcgcg cgctcgtcca tccggaaggt cacctggatg ggcaggtcgg
     4441 tcggcagctc gatctgccgg aagaggctca gcccgaggat gcccaccccc aagccgacga
     4501 cgcctccggc aagggccgtc aacaggctct cgatcaccag ctggcggacc aggcggcgcc
     4561 ggcccgcgcc gatcgcgagc cgcatcgcca tctcgcgggc gcgaaccggg gccctgctcg
     4621 tcagcagccc cgcgacattc gcgcaggcca ccagcaggac cgcggcggca agggtggcca
     4681 gcatcgccac gagcatggca tccggagggc tctgcgcgac gcgagcctgc agttcggtgt
     4741 gcacggccca ctggcggttc ttgttggtgt cgggataggc gcgctcgaga tcggcgccga
     4801 tggcggtcag ctctgcccgg gcgctggcct gcgaggcgcc gggcgcgagg cgccccttga
     4861 gcctcaggtt ccgcgcgtcg cgggcctcga gcgatgccgc cttcgggtcg ttcagcatca
     4921 gcggcgacgt catcagcgga acgaagaaat cggcccggac gtactgatcg aggccggtga
     4981 accccggggg cgccacgcca atcactgtga aaggccggcc gttgaaatcc acgcggcggc
     5041 cgatgaccga cgggtcagac ccgaactgct gctgccacag ggcgtgcccg agcaccacga
     5101 ccgtatcgcg ccccggaacg cggtcctcct ccggcccgaa gccccgcccg agcgccggct
     5161 tgacgcccat gacggagaac aggttgccgc tggccagcat cccgatcttc agcgagggca
     5221 cctcgcccgg gcggctggcg aaacccgcgg tcacgtacga gaaggcggcc actcccgcga
     5281 agctcttgct ccggtcgcgg atgtccacgt aatcgcggta cgacgagttg aggccgctgg
     5341 agccgagcgc ctcgatcgag gtggtcgacc cgagggtcag gatctctccc ggccgcgcga
     5401 ccgggagcgg gcggagcagc aacgcgtcgg cgaagctgaa gatcgcgcag ttggcgccga
     5461 tgccgatcgc cagcgacgcg accgcaatcg cggtgaaccc gggcgaccgc gccaacatgc
     5521 ggccgccgta ccgcacgtcg cgccagaggt gctccagcca gatcggcagc atatggacgt
     5581 atacgcagac cgggggtgga gggtttcggc gggcccacgc cacctgaagc ggcaggcgtt
     5641 tctcagagcg cgatcgtccg cccctccttg cgcgactggt cggcggcgag cacgatccgg
     5701 aggctgtcgt acgccccctg ccagtggtcg gacaggtcga ggtcgtcccg gacggcgcgg
     5761 aggaagaact gctgctcgcg atggcacagc tcctcgtggt cgggttcgtc ctcgatgggg
     5821 atggtctcgt cgggccgggc gaacgccccg tcggcggcga gctccgagtg gtgcaccagc
     5881 agcgcctgcg tttgcgtgtg cgcgctgacg tcggctga
//
LOCUS       DAOPQX010000218         5890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14803_length_5890_cov_4.234619, whole genome shotgun sequence.
ACCESSION   DAOPQX010000218 DAOPQX010000000
VERSION     DAOPQX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14803_length_5890_cov_4.234619"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKK95_11590"
     CDS             <1..369
                     /locus_tag="PKK95_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18906.1"
                     /translation="REIGDHSQAVSLLFEAPEPFLDATRGITSREQLLTGRDEFVVKA
                     GKHGLLFEDIDEKGWPIAVRVGRHTSAILQVAETWSEDNPAKPLAISSVPRYAEVIEK
                     GVGAFLQDVAKAPKDRLAYE"
     gene            529..4611
                     /locus_tag="PKK95_11595"
     CDS             529..4611
                     /locus_tag="PKK95_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC18907.1"
                     /translation="MLLGTPRRPLTHLRVATALALALGFGAWRAEALDPSVGLSQYHT
                     LNWQIENGLPQNSVQALLQSSEGYIWIGTQAGLARFDGIRFVVFDRANTPAMGRENIR
                     ALAEDVDGSIWAATDSGLLRFRRGDSTRISAADGLPSNQVRSLLVDRQGVLWVGTENG
                     VCRLKDGRLLAAKVPGLEGVIPVRIRQSEDGAIFFATMRGLYRLHEGKVARFGTADGL
                     PDEAVFDVCRDRRGTLWVGTSRGAARMVNGRFARLALPSDVADDTVHAVWQDRQGSVW
                     LGLERRGIVRWHGDGGRVEVYGQAQGLAGAYVREFVEDRQGNVWVATFDAGLTCLRET
                     PFSGFGKREGLPTDDVQAILQSRDGDVWMGLNTGGVARVRNGLVTTYSTGDGLNDDTV
                     YSIAEGPGDDIWVGTPRGLNRIWGGRITSVRDSGQLLAGGARALAWMPDGTMWIGTSR
                     GGVWRLEGDRLEPLKADGDRISPLIYVLLPDGQGGAWAGGNRGLSHIKDGRVRTYTTK
                     DGLGDDVVLSLHQDADGSLWIGTFGGGLSRLTKDGIRTLTIRQGLYDDAVFAILEDEA
                     RALWMSCNKGLFRVSRADLDAFFGGRATRVHSPSYGAADGLRGAEGNGGSQPSAWKMR
                     DGRLWFPTIRGAAIVDPTSTHTAVPEVTIELVAHDSRMVAMADALELPPGDGDLEFQY
                     TALDFSAPQGIEFRYRLAGFDTAWVQPGGRRAAFYTHVPPGRYVFEVMARNKDGDWSG
                     APARLPVRLRPHYYQATWFWALCGFGVVAFAVGVYALRVRGMEARAARLAVLVDDRTR
                     ELRAEIEERERAQARLEQEIVERRQVQEELGRAKERAEAANRAKDIFLANMSHEIRTP
                     MNGIIGMTGVLLDTELTAMQRDYAETVKNCADSLLTLLNDILDLSKIEAGKLDLEALD
                     FDLRGTLEDINEVLAIRAHEKGLELTALIDREVPSMVRGDPGRLRQVITNLVGNGIKF
                     TEQGEVSLRVTVLEQDESNVTLRFAVRDTGIGIPSDKLALLFKPFSQVDASTTRRFGG
                     SGLGLSIAQRLVTLMGGTVGVESHEGRGSTFWFTARLGRRPALAAAVPPRLAPDLKGL
                     RVLVVDDNETNHEVLRIMLEAWECRHDHAYNGAAALDRLRAAAMQNDPYRVALLDMMM
                     PGMDGEELGSRVASDPVLSSTQLVMLTSIGLRGDLRRLDPEPFAAYLTKPVRQAQLYD
                     CLRTVVGRVTRPEPKVAVFTPARPDTLSPRLRILVAEDNRVNQKVASRMLEHMGHRVD
                     VVANGLEALAALRAMAYDLVLMDVQMPEMDGFEATRQIRDPAAGVTNPRVPIIAMTAH
                     AMKGDRERCLEAGMDGYVSKPVEPAALREAIEELGGVTIE"
     gene            complement(4711..5115)
                     /locus_tag="PKK95_11600"
     CDS             complement(4711..5115)
                     /locus_tag="PKK95_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOC18908.1"
                     /translation="MNRVFVVVALALAVAFGTGIAVQGQAEKAPGTVILKGAPLGGVK
                     FDHTKHVKLAADKCETCHHPSKPAMPLKAPHQKCSDCHQKAATAQVKTIYRAAFHDPM
                     AKKGTCIDCHQKNVAGGKAPTKCNGCHMKSNT"
     gene            complement(5187..>5890)
                     /locus_tag="PKK95_11605"
     CDS             complement(5187..>5890)
                     /locus_tag="PKK95_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOC18909.1"
                     /translation="FGEYSVFGAGECTFCIASPPTRRGYIECSFKAVGKVTRALRDLE
                     VGEIVGFRGPYGNAFPVEDWSGRRVLFVAGGIGLAPVRCVLWNVLDLRDRFDEVTLVY
                     GARSVADLVYKRELAGWRDRPDVRLVTTVDPGGEEPGWDGEVGFVPAVLERAAPAGDN
                     AVAVVCGPPIMIKHTLPVLARLGFPPDRIFTTLENRMKCGVGKCGRCNVGPVYVCKDG
                     PVFTAAELAALPAEY"
BASE COUNT          880 a         2044 c         2093 g          873 t
ORIGIN      
        1 cgcgagatcg gcgatcactc gcaggccgtc tcgctgctgt tcgaggcgcc cgagccgttc
       61 ctcgacgcca cccgcggcat cacctcgcgc gagcagctgc tgaccggccg ggacgagttc
      121 gtggtcaagg ctggcaaaca cggcctgctc ttcgaggaca tcgacgagaa ggggtggccg
      181 attgccgtcc gcgtggggcg ccacacctcg gcgatcctcc aggtggccga gacctggagc
      241 gaggacaacc ccgcgaagcc gctcgcgatt tccagcgtgc cccggtacgc cgaggtcatc
      301 gagaagggcg tcggggcgtt cctgcaggac gtggcaaagg ctcccaagga ccgcctcgcg
      361 tacgagtagc tcagccgggc gccggcgcgc gctgccccgg cgcgccccgt tcctgcacgc
      421 ttttccctgc cttgtcgtaa agcccgaggg tattccggcc gattctgacg agtaggggcc
      481 gggtattccc acctccgccc cgcgtttctc taccgttcgg agatttcgat gctcctcggg
      541 acgcctcggc gccctctcac ccacctgcgg gtggcgacgg cccttgccct ggcactcggc
      601 ttcggcgcct ggcgggcgga ggccctcgac ccatccgtcg gcctctccca gtaccacacc
      661 ctgaactggc agatcgagaa cgggctgccg cagaacagcg tccaggccct gctgcagtcg
      721 agcgagggct acatctggat cggcacccag gcgggcctcg cccggttcga cggcatccgg
      781 ttcgtggtct tcgaccgcgc caacaccccc gcgatggggc gcgagaacat ccgcgcgctg
      841 gccgaggacg tggacggctc gatctgggcc gccaccgact cgggcctgct ccggttccgc
      901 aggggcgact ccacccggat ctcggctgcc gacggcctgc cctcgaacca ggtccgcagc
      961 ctcctggtcg atcgccaggg ggtgctgtgg gtgggcaccg agaacggagt ctgccgcctc
     1021 aaggacggac gcctcctggc agcgaaggtg cccggcctcg agggcgtcat ccccgttcgc
     1081 atccgccagt ccgaggacgg cgccatcttc ttcgccacca tgcgcggcct ctaccggctg
     1141 catgaaggga aggtcgcccg cttcggcacc gccgacgggt tgcccgacga ggctgtcttc
     1201 gacgtttgcc gcgaccgtcg gggcaccttg tgggtgggga cgagccgcgg ggcggcccgg
     1261 atggtgaacg gccgcttcgc ccgcctcgcg cttccctccg acgtggcgga cgacacggtg
     1321 cacgccgtgt ggcaggaccg ccagggcagc gtgtggctcg ggctcgaacg ccgcggcatc
     1381 gtccgctggc acggcgacgg cggcagggtc gaggtctacg gccaggccca ggggctggcg
     1441 ggcgcatacg tccgtgagtt cgtcgaagac cgccagggga acgtctgggt ggccaccttc
     1501 gacgcgggcc tcacctgtct tcgcgagacg cccttctcgg ggttcggaaa acgcgagggg
     1561 cttcccaccg acgacgtcca ggccatcctc cagtcgcggg atggcgacgt gtggatgggg
     1621 ttgaacaccg ggggagtcgc gcgagtgcgg aacggcctcg tcaccaccta ctccaccggc
     1681 gacgggctca acgacgacac ggtctactcg atcgcggaag gtcccggcga cgacatctgg
     1741 gtggggacgc cgcggggcct caaccgcatc tggggtggcc gcatcaccag cgttcgcgac
     1801 agcgggcagc tgctggccgg cggggcccga gccctcgctt ggatgccgga cgggaccatg
     1861 tggatcggca cctcgcgcgg cggcgtgtgg cgcctcgaag gcgaccggct cgagccgctg
     1921 aaggcggacg gcgacaggat ctcccctctc atctacgtgc tgctgcccga cgggcagggg
     1981 ggcgcctggg ccggggggaa ccgcggcctc agccacatca aggatggccg cgtccgcacc
     2041 tacaccacca aggacggcct gggcgacgac gtggtgctgt ccctgcacca ggacgccgac
     2101 ggctccttgt ggatcggcac ctttggcggc ggcctcagcc gcctgaccaa ggacgggatt
     2161 cgcacgctca cgatccgcca gggcctctac gacgacgccg tcttcgccat cctcgaggac
     2221 gaggcccgcg cgctctggat gtcctgcaac aaggggctct tccgggtgag ccgggccgac
     2281 ctcgacgcct tcttcggcgg gcgtgcgacg cgcgtgcact cgccgagcta cggcgcggcc
     2341 gacgggttgc gcggagccga gggcaacggg ggctcgcagc cgtccgcatg gaagatgcgc
     2401 gacggccgcc tgtggttccc caccatccgc ggcgcggcca tcgtcgatcc gacgtccacc
     2461 cacacggcgg tgcccgaggt gaccatcgag ctggtggcgc acgactcgcg gatggtggcg
     2521 atggccgacg cgctcgagct gccgcccggc gacggcgacc tcgagttcca gtacacggcg
     2581 ctcgacttct cggcgccgca gggcatcgag ttccggtatc gcctcgccgg gttcgacacg
     2641 gcctgggttc agcccggggg gcgacgcgcg gccttctaca cgcacgtccc gccggggcgg
     2701 tacgtgttcg aggtgatggc gcgcaacaag gacggcgact ggagcggcgc accggccagg
     2761 ctgcccgtca ggctgcggcc gcactactac caggcgacct ggttctgggc gctctgcggc
     2821 ttcggcgtcg tggcgttcgc ggtaggcgtc tacgcgctgc gcgtgcgtgg catggaggcg
     2881 cgggcggcgc ggctggccgt gctggtggac gatcgcacgc gcgagcttcg ggccgagatc
     2941 gaggagcgcg agcgggcgca ggccaggctc gagcaggaga tcgtcgagcg gcggcaggtg
     3001 caggaggagc tgggccgcgc caaggagcgg gccgaggcgg ccaaccgggc gaaggacatc
     3061 ttcctggcca acatgagcca cgagatccgg acgccgatga acggcatcat cggcatgacc
     3121 ggcgtgctgc tcgacaccga gctgaccgcg atgcagcgcg actacgccga gacggtgaag
     3181 aactgcgccg actcgctgct gacgctgctg aacgacatcc tcgacctgtc gaagatcgag
     3241 gccgggaagc tcgacctcga ggccctcgac ttcgacctgc gcgggacgct cgaggacatc
     3301 aacgaggtgc tcgccatccg cgcccatgag aaggggctcg agctgacggc cctgatcgac
     3361 cgggaagtgc cctcgatggt acggggcgac ccgggccgcc tgcggcaggt gatcaccaac
     3421 ctcgtcggga acgggatcaa gttcaccgag cagggcgagg tgtcgctgcg cgtgaccgtc
     3481 ctggagcagg acgagtcgaa cgtgacgctg cgcttcgcgg tgcgcgacac cgggatcggc
     3541 attccctccg acaagctggc gctcctgttc aagccgttca gccaggtgga cgcttccacc
     3601 acccggcgct tcggcggctc gggcctgggc ctgtcgattg cgcagcggct cgtcacgctg
     3661 atgggaggga cggtgggcgt cgagagtcac gagggccgcg ggtcgacctt ctggttcacc
     3721 gcccgcctgg gccgccgtcc tgcgctcgcg gcggcagtcc ccccgcgcct cgcgcccgac
     3781 ctgaaggggc tgcgggtgtt ggtggtggac gacaacgaga ccaaccacga ggtgctgcgt
     3841 atcatgctgg aggcgtggga gtgccggcac gaccacgcct acaacggggc cgcggcactc
     3901 gaccggctgc gcgccgcggc catgcagaac gacccctacc gggtggcgct gctcgacatg
     3961 atgatgcccg gcatggacgg cgaagagctg ggcagccgcg tggcgtcaga cccggtgctg
     4021 tcgagcacgc agctggtgat gctcacgtcg attggcttga ggggcgacct gagacgcctc
     4081 gacccggagc cgttcgccgc ctacctgacc aagccggttc gccaggcgca gctgtacgac
     4141 tgcctgcgca cggtggtcgg ccgggtcacg cggcccgagc cgaaggtggc ggtcttcacc
     4201 ccggcccgtc ccgacacgct cagcccgcgc ctgcggatcc tggtcgccga ggacaaccgg
     4261 gtgaaccaga aagttgcctc gcggatgctc gagcacatgg gacaccgggt cgacgtcgtc
     4321 gcgaacggcc tcgaggcgct cgcggcgctg cgggcgatgg cctacgacct ggtgttgatg
     4381 gacgtccaga tgcccgagat ggacgggttc gaggcaaccc gccagatccg cgacccggcg
     4441 gccggggtca ccaacccccg tgtcccgatc atcgccatga cggctcacgc gatgaagggc
     4501 gaccgcgagc gatgcctcga ggcgggaatg gatggctacg tgtcgaagcc ggtcgagccg
     4561 gcggcgctgc gcgaggcgat cgaggagctg ggcggggtga cgatcgagta gggccggcct
     4621 tcacgcgtag agctgggctc tagccctgct gcgtgttcgt cctcaccggg tggagcaggg
     4681 cttcagccct gctccagcgg caacacccct ctacgtgttc gacttcatgt ggcagccgtt
     4741 gcacttggtc ggcgccttcc cgccggccac gttcttctga tggcagtcga tgcacgtgcc
     4801 cttcttcgcc atcgggtcgt ggaaggccgc ccggtagatc gtcttcacct gcgccgtcgc
     4861 ggccttctga tggcagtcgc tgcacttctg gtgcggcgcc ttcaacggca tcgcaggctt
     4921 cgacgggtga tggcacgtct cgcacttgtc ggccgcgagc ttgacgtgct tggtgtgatc
     4981 gaacttgacg ccgccgagcg gcgcgccctt caggatgaca gtgcccggag ccttctcggc
     5041 ctggccctga acggcaatgc ctgtcccgaa cgctacagcc agggccaggg cgacaaccac
     5101 gaacactcga ttcatgctgc ctcctccacg acaggatgtc gggcttggca gcatcgtagc
     5161 acagggcggg gttcccagcc ccgaggctaa tattccgcgg ggagggcggc cagctcggcg
     5221 gcggtgaaga ccggcccgtc cttgcacacg tagaccgggc cgacgttgca gcgcccgcac
     5281 ttgccgacgc cgcacttcat ccggttctcc agcgtggtga agatgcggtc gggcgggaag
     5341 ccgagccgcg cgagcaccgg gagcgtgtgc ttgatcatga tgggagggcc gcagaccacc
     5401 gcgacggcgt tgtcgcctgc aggggcggca cgttcgagca cggccgggac gaagcccacc
     5461 tcgccgtccc accccggctc ctccccgccg ggatcgacgg tcgtcacgag gcggacgtcg
     5521 ggccggtcgc gccagcccgc cagctcgcgc ttgtagacca ggtcggcgac gcttcgcgcc
     5581 ccgtagacca gcgtcacctc gtcgaaccgg tcgcgcaggt cgagcacgtt ccagaggacg
     5641 caccgcaccg gggccaggcc gatgccgccc gcgacgaaca ggacgcgccg gcccgaccag
     5701 tcctcaacgg ggaaggcgtt cccgtagggg ccgcgaaacc ccacgatctc gcccacctcc
     5761 agatcccgga gggcgcgcgt caccttgccg acggccttga agctgcactc gatgtagccg
     5821 cggcgggtcg gcgggctggc gatgcagaag gtgcactcgc cggcgccgaa aaccgagtac
     5881 tcgccgaact
//
LOCUS       DAOPQX010000219         5882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14830_length_5882_cov_5.113781, whole genome shotgun sequence.
ACCESSION   DAOPQX010000219 DAOPQX010000000
VERSION     DAOPQX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14830_length_5882_cov_5.113781"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /gene="malQ"
                     /locus_tag="PKK95_11610"
     CDS             complement(<1..1263)
                     /gene="malQ"
                     /locus_tag="PKK95_11610"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOC18910.1"
                     /translation="MTHPRPLQGRHGGLVVPLFSIPSRASWGIGEIPDIPLFASWLTS
                     AGQDLLQLLPINEVAVGQTSPYSAMTAMAIDPMYIGLRDAADFQAAGGEGAMDAAWRE
                     GLERARQCRVVDYGLVGKLKRQALRAAFETFREAEWARGTPRAEAFRRWGDEQAWWLD
                     DYALFRALHARESERAWTDWPDGLRTREAGALEEASRELAGEILYRRWLQWIADEQWQ
                     AAKRAMRPVALLGDLPFMVDGDSADVWANADAFRTDASVGAPPDAFSETGQNWGLPAY
                     RWDVMGSRDFAWLRARARRSADLYDGYRVDHLVGFYRTYVFPRGDEKPFFTPATEDEQ
                     LRLGETLLRLFAEPGAQIIAEDLGTIPTFVRASLARLGVPGYKVFRWEREWDEPGQAF
                     RDTAAYPAVSVAASGTHDTETVAEWWDSL"
     gene            complement(1275..2438)
                     /locus_tag="PKK95_11615"
     CDS             complement(1275..2438)
                     /locus_tag="PKK95_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOC18911.1"
                     /translation="MFSSRTPADLAPNALARALAALRARGEDVLDLTETNPTRAGLTA
                     PPELLVPLAGPASLAYEPHPFGLPAAREAVASTYQAAGLALPAAHVVLTSSTSEAYSF
                     LFKLLCDPGDEVLVPVPSYPLFDHLTRLDGVVPRPYPLEYHGSWSIDLEALERSVGPR
                     TRAILVVSPNNPTGSVLRQGDLDRLSALAASAGLAIVGDEVFADYPLEPAGNGCRVLA
                     QAGALTFALGGLSKSVGLPQLKLAWIAASGPPGLLEPALARLEVVADAYLSVATPVQQ
                     AAGSLLAAGQAVHQAIAGRVRRNLGHLREASAASPCSVLRVEGGWSAVLRVPATRPEE
                     TIVLDLLEQDGVLVHPGYFFDFPREAFLVLSLLPPPASFDEGVSRLIARLSLG"
     gene            2523..3926
                     /locus_tag="PKK95_11620"
     CDS             2523..3926
                     /locus_tag="PKK95_11620"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC18912.1"
                     /translation="MLPFSTGRRARAWLAAAAALALAAPAAVSAQPAAPATLTTDGPA
                     PIEVPYTQFTLPNGLNVILHEDHTVPVVSVNVWYHVGSGREKPGRTGFAHLFEHLMFM
                     GSGHVKEGEFDRLLEAAGGNNNGSTNEDRTNYWIDVPSNALELALFLESDRMGYLLDA
                     MTPASVDAQRDVVKNERRQSYENRPYGMASIVLSELLFPASHPYHWPTIGYMPDLSAA
                     SYEDVVEFFKKYYAPNNASLSIAGDIDVAATRKLVERYFGEIPRGAFVEPLAPPAAFL
                     TSVVTKTLTDKVQLPRLYLAWHTPALLTPGDAALDAVASILAGGKNSRLYRRLVYDMQ
                     IAQNVVAMQGSQALGSVFMVVVTPRPGHALDEVQKVVDEEIGRLRDEPPSARELQRVI
                     NQTEASFYARMERVGSFGGKADQLNAYYTGTGNPDYYAEDLARYRALSPSDVQAAVRQ
                     FLPADRRVELRVEPEKN"
     gene            3937..5370
                     /locus_tag="PKK95_11625"
     CDS             3937..5370
                     /locus_tag="PKK95_11625"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC18913.1"
                     /translation="MRFERLLFVPLLFLAAGAALAQAPDRTRVPVPGPAPELRLPEIQ
                     KRALSNGLQVWIVEQHEVPVVQVSLVIGAGGNADPAGKPGLASMTAAMLDEGAGQRSA
                     LEIADAIDFLGASLATSGSFDASAVRLWVPVSRLADALPVMSDVAIRPTFPPDELERL
                     RKERLTAMLQARDDPAQVASLSFPRFVFGQHRYGTSLQGTPASVGAFTPEDLRSFYEG
                     HYRPERSTLVVVGDVKTDEALRLLESAFGGWKGKGPAAAAPALPEAPRAKARQVFIVD
                     KPGAPQSQIVIGAVGVPRSTPDYFPLQVLNTLLGGSFTSRLNQNLREEHGYTYGARSA
                     FDMRLAAGPFLARAGVQTEVTAEALTEFFKELNGVLEPIPPDELSRAKNYVALGFPSE
                     FETTGDIAARLEELIVYELPKEYYSSYVERIQAVTAQDVRRVAQRYITPDRFTVVVVG
                     DRKAIEAPVRGLKLGPVTVMSADEALK"
     gene            5376..5618
                     /gene="nadA"
                     /locus_tag="PKK95_11630"
     CDS             5376..5618
                     /gene="nadA"
                     /locus_tag="PKK95_11630"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOC18914.1"
                     /translation="MISPTLDLFAEIARLKQELDAVILAHYYQEPDIQDIADHVGDSL
                     ALAQAAEKAKERTIVFCGVHFMAETAKILNPGKLIP"
     gene            5615..>5882
                     /locus_tag="PKK95_11635"
     CDS             5615..>5882
                     /locus_tag="PKK95_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOC18915.1"
                     /translation="MTIGRVTVLTNPASGHGSAPHAAERAITQLHRRGVDVVAIAGRD
                     SAHARQLVEGALERGMDALVVVGGDGIISLALQVLARTDIPLGLI"
BASE COUNT          914 a         2078 c         2080 g          810 t
ORIGIN      
        1 gagcgagtcc caccattccg cgacggtctc ggtgtcgtgc gtgcccgagg cggcgaccga
       61 caccgccggg taggccgccg tgtcgcgaaa cgcctgcccc ggctcgtccc attcgcgctc
      121 ccaccggaac accttgtacc cgggcacgcc gagccgggcc agcgaggcgc gcacgaaggt
      181 cgggatcgtt cccaggtcct cggcgatgat ctgcgcccct ggctccgcga acaggcgaag
      241 cagcgtttcg ccgagccgca gctgctcgtc ctcggtggcg ggcgtgaaga acggcttctc
      301 gtcgccgcgg gggaacacgt aggtgcggta gaacccgacg aggtgatcga cccggtagcc
      361 gtcgtagagg tcggcgctcc ggcgcgcccg ggcgcgcagc caggcgaagt cacgcgagcc
      421 catcacgtcc caccggtaag ccggcagccc ccagttctgc ccggtctcgc tgaaggcgtc
      481 cggcggcgcg cccaccgagg cgtcggtcct gaaggcgtcg gcgttggccc agacgtcagc
      541 gctgtcgccg tccaccatga acggcaggtc gccgagcagc gccaccgggc gcatcgcacg
      601 cttggcagcc tgccactgct cgtcggcaat ccactgcagc cagcgccggt acaggatctc
      661 cccggccagc tcgcgggaag cctcctcgag cgcgccggcc tcgcgggtgc gcaagccatc
      721 gggccagtcg gtccacgcgc gctctgactc gcgcgcgtgc agcgcccgga aaagcgcgta
      781 gtcgtcgagc caccaggcct gctcgtcgcc ccaccgcctg aaggcctccg cacggggggt
      841 cccgcgggcc cactcggcct cgcggaacgt ctcgaaggcg gcgcgaagcg cctgcctctt
      901 cagcttcccc acgagcccgt agtccaccac ccggcattgc cgcgcccgct cgagcccctc
      961 ccgccacgcg gcgtccatcg ccccctcgcc cccggcggcc tggaagtcgg ccgcatcgcg
     1021 caggccgatg tacatcgggt cgatggccat cgccgtcatc gccgagtagg gcgaggtctg
     1081 gcccacggca acctcgttga tcggcagcag ctgcaacagg tcctggccgg cactggtcag
     1141 ccacgaggca aagagcggga tgtcggggat ctcgccgatg ccccagctgg cccgcgaggg
     1201 gattgagaag agcggcacga cgaggccgcc gtggcggccc tggagcggtc gggggtgtgt
     1261 cacggtgcgc accgttagcc gagcgacagc cgagcgatca gccgcgacac cccttcgtcg
     1321 aaggacgcgg ggggcggcag gaggctgagc acgaggaacg cctcgcgcgg gaagtcgaag
     1381 aagtaacccg ggtggaccag cacgccgtcc tgctcgagca ggtcgagcac gatcgtttcc
     1441 tcgggccgcg tggccggcac ccgcagcacc gccgaccatc caccttcgac gcggagcacc
     1501 gagcagggcg acgcggcgga ggcttcgcgc aggtgcccga ggttgcgtcg cacgcgaccg
     1561 gcaatcgcct ggtggacggc ctgcccggct gcaaggagcg agccggcagc ctgctgcacc
     1621 ggcgtcgcga ccgacaagta cgcgtcagcg acgacctcca gtcttgccag cgccggctcg
     1681 agcagcccgg gcgggccgct ggcggcaatc caagccagct tcagctgggg caggccgacc
     1741 gacttcgaaa gcccgccgag tgcgaaggtg agcgcgccgg cctgcgcgag cacccggcag
     1801 ccgttgccgg ccggctcgag ggggtagtcg gcgaacacct cgtcgccgac gatggccagc
     1861 ccggcagagg cggcgagcgc cgacaggcgg tcgaggtcgc cctggcgcag gacgcttccg
     1921 gtcgggttgt tcgggctcac caccaggatc gcccgggtgc gcgggccgac cgagcgctcc
     1981 aaggcctcga ggtcgatgga ccacgacccg tggtactcga gcgggtaggg ccgcggaacc
     2041 accccgtcca gccgcgtcag gtggtcgaag agcgggtagc tgggcaccgg gaccagcact
     2101 tcgtcgcccg ggtcgcagag cagcttgaag aggaaggagt acgcctcgct cgtactcgag
     2161 gtgagcacga cgtgggcggc gggcaaggcc aggccggcgg cctgataggt ggaggcgacc
     2221 gcctcgcggg cggcgggcaa cccgaacggg tgcggctcgt aggcgagcga ggccggtccg
     2281 gcgagcggca ccagcagctc gggcggagcg gtgaggccgg cccgcgtcgg gttcgtctcg
     2341 gtgagatcga gcacgtcctc accgcgtgcg cgaagcgccg caagggcccg ggcgagcgcg
     2401 ttgggcgcaa ggtcggcagg ggtgcgcgag gaaaacatgg tcaggcaact acgttaaact
     2461 acggcgaggg ttgaagacac ggtttttgcc gcgcggcggt cgccgcgcga ggagctcacg
     2521 cgatgcttcc cttctcaacc gggcggcgtg cccgggcctg gctcgccgcc gcagcggccc
     2581 ttgcgctggc cgcgcccgcg gcggtttccg cccagccggc ggccccggcc acgctcacga
     2641 cggatgggcc ggcgccgatc gaggtcccct acacgcagtt cacgctgccc aacggcctca
     2701 acgtgatcct ccacgaggat cacaccgtgc cggtggtctc ggtgaacgtg tggtaccacg
     2761 tggggtcggg gcgcgagaag ccgggccgca ccgggttcgc ccacctgttc gagcacctga
     2821 tgttcatggg ctcggggcac gtgaaggaag gggagttcga ccggctgctc gaggcggcgg
     2881 gcggcaacaa caacggctcg accaacgagg accggaccaa ttactggatc gacgtcccgt
     2941 cgaacgcgct cgagctggcc ctcttcctcg agtccgaccg catgggctac ctgctcgacg
     3001 ccatgacgcc ggcctcggtc gacgcgcaac gtgacgtcgt caagaacgag cgccgccaga
     3061 gctacgagaa ccggccgtac gggatggcgt cgatcgtcct gagcgagctg ctcttccccg
     3121 cgagccaccc gtaccactgg ccgacgatcg gctacatgcc cgacctctcg gcggccagct
     3181 acgaggacgt cgtcgagttc ttcaagaagt actacgcccc gaacaacgcg agcctgtcga
     3241 tcgccggcga catcgacgtc gcggccacgc ggaagctggt cgagcggtac ttcggcgaga
     3301 tcccgcgcgg cgccttcgtc gagccgctcg ccccaccggc ggcgttcctg acgtccgtgg
     3361 tgacgaagac gctgaccgac aaggtgcagc tgccccggct ctacctcgcg tggcacaccc
     3421 cggcgctgct cacccctggc gacgccgcgc tcgacgcggt cgcgtcgatc ctggcgggcg
     3481 gcaagaactc gcgcctctac cgccgcctcg tctacgacat gcagatcgcg cagaacgtgg
     3541 tggcgatgca gggctcgcag gcgctgggca gcgtgttcat ggtcgtcgtg acgccgaggc
     3601 ccggacacgc gctggacgag gtgcagaagg tcgtcgacga ggagatcggg cggctgcgcg
     3661 acgagccgcc gtccgcccgg gaactgcagc gggtgatcaa ccagaccgag gcgtccttct
     3721 acgcgcggat ggagcgggtg ggctcgttcg gcggcaaggc cgaccagctg aacgcctact
     3781 acaccgggac tggaaacccc gactactacg ccgaggacct ggcgcgctac cgggcgctgt
     3841 cgccctcgga cgtgcaggcc gcggtccggc aattcctgcc agcggaccgc cgggtcgagc
     3901 tgcgggtgga gcccgagaag aactagggtg ttgcacatgc gcttcgagcg actgctcttc
     3961 gttcccctgc tgttcctcgc ggccggcgct gcccttgcgc aggcccctga ccgcacgcgg
     4021 gttcccgttc ccggccccgc gcccgagctt cgcctgcccg agattcagaa gcgcgcgctc
     4081 tcgaacggcc tgcaggtgtg gatcgtcgaa cagcacgagg tcccagtcgt ccaggtgagc
     4141 ctggtgatcg gcgccggcgg gaacgccgac ccggcgggca aacccggcct cgcgagcatg
     4201 acggcggcga tgctggacga gggggcgggc cagcggtcgg cactggagat cgccgacgcg
     4261 atcgacttcc tcggcgcctc tttggccacc agcggcagct tcgacgcctc cgccgtgcgg
     4321 ctctgggtcc cggtctcacg ccttgccgac gcgctgccgg tgatgtctga cgtcgctatc
     4381 cgtcccacgt ttccgcccga cgagctggag cggcttcgaa aggagcggct gaccgcgatg
     4441 ctgcaggcgc gcgacgaccc ggcgcaggtc gcaagcctgt cgttcccaag gttcgtcttc
     4501 ggccagcacc gctacggcac ctccctccag gggacgccgg ccagcgtggg cgccttcacg
     4561 ccggaggacc ttcgctcgtt ctacgagggc cactatcgcc ccgagcgttc cacgctcgtc
     4621 gtggttggcg acgtgaagac agacgaggcg ctgcgcctgc tcgaatcggc cttcggcgga
     4681 tggaagggga aggggccggc ggctgcggct ccggcgctgc cggaagcgcc gcgcgccaag
     4741 gcccggcagg tcttcatcgt cgacaagccg ggcgcgccac agtcgcagat cgtgatcggc
     4801 gcggtcgggg tgccgcgctc gacgcccgac tacttccctt tgcaggtgct gaacacgctg
     4861 ctcggcggct cgttcacctc gcggctgaac cagaacctgc gcgaggagca cggctacacc
     4921 tacggcgcga ggtcggcgtt cgacatgcgc ctcgctgccg ggccgttcct cgccagggcc
     4981 ggcgtgcaga ccgaggtgac cgcggaggcg ctcaccgagt tcttcaagga gctgaacggg
     5041 gtgctcgagc cgatcccgcc cgacgagctg tcgcgagcga agaactacgt ggcgctcggc
     5101 ttcccgtcgg agttcgagac cacgggcgac atcgcggcgc gcctcgaaga gttgatcgtg
     5161 tacgagctgc ccaaggagta ctactccagc tacgtggagc ggatccaggc cgtgacggcc
     5221 caggacgtcc gccgggtcgc ccagcgctac atcacgccag accgcttcac ggtcgtcgtg
     5281 gtgggcgacc ggaaggcgat cgaggcgccg gtgcggggcc tgaagctcgg ccccgtgacc
     5341 gtgatgtcag cagacgaggc actgaagtga cggagatgat ttcgcccacc ctggacctgt
     5401 tcgccgagat cgcgcggctg aagcaggagc tcgacgcggt catcctggcg cactactacc
     5461 aggaacccga catccaggac atcgcggacc acgtcggtga cagcctcgcg ctcgcgcagg
     5521 ccgccgagaa ggcgaaggag cgcacgatcg tcttctgcgg cgtccacttc atggccgaaa
     5581 cggcgaagat cctgaacccc ggcaagctga ttccgtgacc atcggccggg tcaccgtgct
     5641 gaccaacccg gcatcggggc acggcagcgc accgcatgcc gcggaacgcg cgatcacgca
     5701 gttgcaccgc cgtggggtgg acgtggtggc catcgcgggt cgcgactcgg cgcatgcccg
     5761 ccaactcgtc gagggcgcac tggaacgcgg catggacgcg ttggtcgtcg tcggcggcga
     5821 cggcatcatc tcgctggccc tgcaggtgct cgcccggacc gacattcccc tcggcctcat
     5881 cc
//
LOCUS       DAOPQX010000220         5854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14937_length_5854_cov_5.504052, whole genome shotgun sequence.
ACCESSION   DAOPQX010000220 DAOPQX010000000
VERSION     DAOPQX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14937_length_5854_cov_5.504052"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PKK95_11640"
     CDS             <1..500
                     /locus_tag="PKK95_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HOC18916.1"
                     /translation="HVFGGAARARTAEEVLGRWEEVKARERQAETSKNTPARSILGGV
                     PRSLPALLRAYEMGTRAAAVGFDWERSADVLDKIDEEVAELREAVERREGIEEEMGDL
                     LFALANLARKLGIEPESALRSADDKFARRFTTMERRFAARGESLKDAGLARMEEEWGR
                     IKEEE"
     gene            complement(525..1301)
                     /locus_tag="PKK95_11645"
     CDS             complement(525..1301)
                     /locus_tag="PKK95_11645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC18917.1"
                     /translation="MRASGAAAAVVLVLMLAGWWGSASGQETSHGGILRGSSHAREIA
                     LEFTGHEYAEGGQVILDQLARRRARASFFFTGDFLRRSEFAPLVRRIVSEGHYLGPHS
                     DKHLLYCGWEASRPTLVSRETFRRDLEDNLAELERFAVRRAGVRYWVPPYEWFNEEIA
                     AWSLEAGLQIVNFTPGTRANADYTGESDRNFVTSQAILDSILERERTGPNGLNGFLLL
                     MHVGAGPGRRDKMHDRLGELLDVLSSRGYRFVRVDELLGR"
     gene            complement(1377..3854)
                     /locus_tag="PKK95_11650"
     CDS             complement(1377..3854)
                     /locus_tag="PKK95_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOC18918.1"
                     /translation="MWSPALVPALALTAGCAIGLLAGGVPAFALGVLVGAAWLTALAA
                     HAVGRPRLFVGAVALTFSLCGWGLAAAADDRARRPPLLDSWRGAEAGAPFRLEGVLRE
                     DATPWGEAVGLSLAADRLGEGEAWRETRGGVRLSVAGALAAERVDGWRAGRRVRLTAR
                     LREPTRYRDPGVRDARVALARRGRILIGGVKSAALVEDVSRGPAATEAAAAVRRRTRA
                     AVARRVGSRSPRSAGIIVAILIGDRAGLDEEVERRLQEAGTYHVIAISGGNIAILAAL
                     AAIVLRSLGVGRRPACLGVIALLVSYAFVVGPEPSVSRATAMAVLYFGARLFDFKTGP
                     VNILAASAAALLAWAPCSASEAGFALTYGATLGIVVGAGVAGTGRAPRWARAPLGLLL
                     ASASAELALIPVSAWAFSRVTFAGLVLNFAAIPLMTAAQVAGMAVVPLDAAWPAAASL
                     AGLVAHLAAEGLVRSADFIQLAPWLSYRLPPPHPVAIALYYLAWAVWLAPPGAGASRV
                     SRLRRPAAMLVGCASLAWILVEPATLLWPGVSGRLRVAVLDVGHADAILVQLPDRRSL
                     LVDTGGSLLGSSFDIGGRVVAPALWALGTRRLDVLVLTHLHPDHAGGALSVIRDFRPR
                     EVWEGISVPALEQLQPLRRAAEQAGAQWHTKLAGERLVFGEVSLSVWHPPPPEWERQR
                     VRNDDSIVLELRFRDVSVVLPGDIGREVERALAPNLPPAPIRVLKVPHHGSATSSTEE
                     FVSALAPRAAVLSAGPSTTVSDAVLRRYADRGVPLFRTDRDGAVLLTTDGRSVTIETY
                     TGRNCTIDRISTTKITKNTKKEESRRR"
     gene            complement(4092..>5854)
                     /gene="carB"
                     /locus_tag="PKK95_11655"
     CDS             complement(4092..>5854)
                     /gene="carB"
                     /locus_tag="PKK95_11655"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOC18919.1"
                     /translation="SRQAGFSLEEIGRLSGVAVPAIEALGEAAGIRRVFKRIDTCAAE
                     FESFTPYLYSTFAAECEADPTPRRKVIILGSGPNRIGQGIEFDYCCCHAAFALREEGV
                     ETVMVNCNPETVSTDYDTADRLYFEPLTFENVMGIVAREREAGGEVSCLVQFGGQTPL
                     KLALPLHRAGVPIIGTSADSIDLAEDRKRFSALLDRLGIAQPASGTALSHEEARAIAG
                     SIGFPVLVRPSYVLGGRGMAIVYDEAALDGYVTRALADAPDSPVLIDRYLEDAFELDV
                     DALADATGHVVIGGIMEHIEQAGIHSGDSSCVLPSYLVPERHLDTIRDWTRRIARELN
                     VVGLMNIQFALKDDQVYVLEVNPRASRTVPFLSKATGVPLAKAAARLMLGRTLAELDL
                     TGDVPVSGTFVKTPVFPFVRFTGVDTLLGPEMKSTGEAMGCGQTFGAAFARAQEGAGA
                     ALPTSGTIFLSVNDRDKPAILQVARGLAALGFSLAATRGTAAFLAANNLPARPIYKVN
                     EGRPNVADAVANGEIALVVNTPLGRASFFDEKALRRAAMLHGVPCLTTMSAAVAAVSA
                     IAAIREGEAEVRALQDYHGA"
BASE COUNT          901 a         2096 c         2130 g          727 t
ORIGIN      
        1 cccatgtctt cggcggcgcc gcccgggccc ggacggccga agaagtgctg ggccgctggg
       61 aggaggtgaa ggcccgcgag cggcaggccg agacctcgaa gaacacaccg gccaggagca
      121 tcctgggcgg cgtcccgcgc tcgctcccgg cgctgctgcg cgcctacgag atgggcaccc
      181 gcgccgccgc ggtcggcttc gactgggaac ggtcggctga cgtcctcgac aagatcgacg
      241 aggaggtggc cgagctgcgc gaggccgtgg agcgccgcga gggcatcgag gaggagatgg
      301 gcgacctgct cttcgccctg gcgaacctgg cccgcaagct cggcatcgag cccgagtcgg
      361 cgctgaggag tgccgacgac aagttcgcgc gacggttcac caccatggag cggcggttcg
      421 cggctcgtgg cgagtcactc aaggacgcgg ggcttgcccg gatggaagag gagtggggcc
      481 ggatcaagga ggaggagtag gaggccgcgt gtttcagcgg cctgctatcg cccgagcagc
      541 tcgtccaccc ggacgaagcg gtagccgcgt gacgaaagga cgtcgagcag ctcgcccagg
      601 cgatcgtgca tcttgtcgcg gcggccaggt cccgcgccca cgtgcatcaa cagcaggaag
      661 ccgttcaggc cgttcgggcc ggtgcgctcc cgctcgagga tgctgtcgag gatggcctgc
      721 gaggtgacga agttcctgtc gctctcgccc gtgtagtcgg cgttcgcgcg cgtgccgggc
      781 gtgaagttga cgatctgcag cccggcctcg aggctccagg ccgcaatctc ctcgttgaac
      841 cactcgtagg gcgggaccca gtaccggacc cccgcgcgac gaaccgcaaa gcgttccagc
      901 tcagccaggt tgtcctcgag gtcgcggcga aacgtctcgc ggctcaccag cgtcggccgc
      961 gatgcctccc acccgcagta gagcaggtgc ttgtccgagt gggggcccag gtagtgcccc
     1021 tccgagacga tccggcgtac gagcggcgcg aactcgctgc gacgcaggaa gtcgcccgtg
     1081 aagaagaacg aggctcgcgc ccgccggcgc gcgagctggt cgaggatgac ctggcctcct
     1141 tccgcgtact cgtggccggt gaactcgagg gcgatctccc gcgcgtgtga cgaaccgcgc
     1201 aggatgcccc cgtggctcgt ctcctggccg ctcgcacttc cccaccagcc ggccagcatc
     1261 agcaccagga ccacggccgc cgcggcgccg ctagctctca tgccggtatc ttgacatgcc
     1321 cacctccttg ccagtccacg aggttcgacc acaagggaca cggtccacct gattcatcac
     1381 ctccgtcgcg attcttcctt ctttgtgttc tttgtgatct ttgtggttga aatccggtca
     1441 atggtgcagt tgcggccagt gtaggtttcg atggtgacgc tgcgcccgtc ggtcgtcagc
     1501 aggacggcgc cgtcgcggtc ggtgcggaac aagggaacgc cgcgatccgc gtagcgccgg
     1561 agcaccgcat cgctcaccgt tgtggagggg ccggcgctga gaaccgccgc gcgcggggcg
     1621 agtgccgaca cgaactcctc ggtgctcgag gtagcgctgc cgtggtgcgg gaccttgagc
     1681 acccggatcg gtgcgggcgg aaggttgggc gcaagggccc gctcgacctc gcgcccgatg
     1741 tcgccgggga gcaccaccga gacgtcgcgg aagcgaagct cgaggacgat cgagtcgtcg
     1801 ttgcgtaccc gctgacgctc ccactcgggc ggaggcgggt gccagacgga gagcgacacc
     1861 tcgccgaaga cgaggcgctc ccctgccagc ttggtgtgcc actgcgcccc ggcctgctcc
     1921 gcggctcgtc gcagcggttg cagttgctcc agggcaggca cggagattcc ctcccagacc
     1981 tcgcgcggac gaaagtcgcg aatcaccgac agggcgccgc cggcatgatc tgggtggagg
     2041 tgcgtcagca cgagcacgtc caggcgcctg gtaccgagcg cccacagcgc gggcgcgacg
     2101 acgcggccgc cgatgtcgaa ggagctgccc agcagcgagc cgccggtgtc caccagcagg
     2161 ctccggcgat cgggcagctg cacgaggatg gcgtcggcat gcccgacatc gagtaccgcg
     2221 acgcgcaggc ggccgctgac gcccggccag agaagcgtcg cgggctcgac caggatccac
     2281 gcgaggctgg cgcagccaac cagcatcgcg gcggggcggc gcaggcgcga gacgcgcgag
     2341 gcgccggcgc ccggcggcgc cagccacacc gcccatgcca ggtaatagag cgcgatagcc
     2401 actgggtgcg gcggcgggag gcggtacgag agccacggag ccagctggat gaaatcggcg
     2461 ctccgcacca acccctcggc ggccaggtgt gccacgaggc ccgccagcga cgccgccgcg
     2521 ggccacgcgg catcgagagg caccacggcc atgcccgcca cctgcgcggc ggtcatcagc
     2581 gggatggcgg cgaagttcag cacgaggccc gcgaacgtga cgcgcgagaa ggcccacgcc
     2641 gacaccggga tgagtgccag ctccgcgctg gccgacgcga gcagcaaccc cagcggcgcc
     2701 cgcgcccagc gcggcgcgcg gcccgttccc gccacgccgg ccccgacgac gatgccgagc
     2761 gtggcgccgt aggtcagcgc gaagccggcc tcgctggcgc tgcacggcgc ccaggccagc
     2821 agcgcggccg ccgacgcggc caggatgttc acggggccgg tcttgaagtc gaacaggcgc
     2881 gccccgaagt acagcaccgc catcgccgtc gcgcggctca ccgacggctc ggggccgacc
     2941 acgaacgcgt acgacaccag cagggcgatg acgcccaggc aggcgggccg ccggccgacg
     3001 ccgagcgagc gtaggacgat cgcggccagc gcggcgagga tcgcgatgtt cccgcccgag
     3061 atcgcgatca cgtgataggt gccggcctcc tgcagccgcc gctcgacctc ctcgtcgagg
     3121 cctgcacggt cgccaatcag gatcgccacg atgatgccgg ccgaccgcgg gctgcgcgac
     3181 ccgacgcggc gcgcgacggc cgcgcgcgtc ctccggcgca cggccgcggc ggcctcggtg
     3241 gccgccggcc ctcgcgacac atcctcgacc agcgcggcgc tcttgacccc cccgatgagg
     3301 atcctcccgc gcctggccag cgccacccgg gcgtcgcgca cgccggggtc tcgataccgg
     3361 gtgggctcgc gcagcctggc ggtgaggcgc acgcgccggc cggcgcgcca cccgtccacg
     3421 cgttcggccg ccagcgcccc cgcgaccgag agccggacgc cgccgcgcgt ctcgcgccag
     3481 gcctcgccct cgccgaggcg atcggccgcg agcgacaggc caaccgcctc gccccacggc
     3541 gtggcgtcct cccggaggac gccctcgagc cggaacgggg cgccggcctc cgcgccgcgc
     3601 caagaatcga gcagcggcgg acggcgtgcg cgatcgtccg ccgcggcggc aaggccccac
     3661 ccgcagagcg agaaggtgag ggcgacggcg cccacgaaga ggcgcggacg gccaaccgcg
     3721 tgagcggcga gggccgtcag ccaggccgcg ccaacgagga cgcccagcgc gaaagccggc
     3781 acgcccccgg cgagcaggcc gatggcgcag ccggcggtta gcgccagcgc gggcaccagc
     3841 gcgggtgacc acacaccggc tcacccagca agacaagcgc cggacggaag ggcgccgcta
     3901 caggcgcagc atcaccggca ggcgggaagg ggtacgcaga aggtgcgatg cgggggcgcg
     3961 agggatgttg cagctaacgc agtcacggag tcacgaagtc gcgaagtcgc gaagtcacga
     4021 ggtcgagtct ggagtcgcaa agtcgcgagg tcgagtcgcg aggtcgagtc ccgcgtcgtc
     4081 gagtctggcc gtcaggcccc gtggtagtcc tgcagggccc gcacttcggc ctcgccctcg
     4141 cggatggccg cgatggcgct gacggcggcc acggcggcgc tcatcgtggt gaggcagggc
     4201 acgccgtgca gcatcgccgc gcggcgcagc gccttctcgt cgaagaacga cgcccgcccg
     4261 agcggcgtgt tcaccaccag ggcgatctcg ccgttggcca ccgcgtcggc gacgttggga
     4321 cgcccctcgt tcaccttgta gatcggccgc gcgggcaggt tgttggccgc gaggaaggcg
     4381 gcggtgccgc gcgtggcggc cagcgagaac ccgagcgccg ccagcccgcg cgcaacctgc
     4441 aggatggccg gcttgtcgcg gtcgttcacg ctgaggaaga tggttcccga ggtcggcagc
     4501 gcggcccccg ccccttcctg cgcccgggcg aacgcggcgc cgaaggtctg cccgcacccc
     4561 atcgcctcgc cggtggactt catctcgggc ccgagcagcg tgtccacccc ggtgaagcgc
     4621 acgaagggga agaccggcgt cttcacgaag gtgcccgaca ccggcacgtc gcccgtgagg
     4681 tcgagttcgg ccagcgtgcg ccccagcatc aaccgcgcgg ccgccttggc gagcgggacg
     4741 ccggtcgcct tcgagaggaa cggcacggtg cgcgaggcgc gcgggttcac ctcgagcacg
     4801 tacacctggt cgtccttcag cgcgaactgg atgttcatca ggccgaccac gttcagctcg
     4861 cgcgcgatgc gccgcgtcca gtcgcggatc gtgtcgaggt ggcgctcggg caccaggtac
     4921 gacgggagca cgcacgagct gtcgcccgag tggatgcccg cctgctcgat gtgctccatg
     4981 atgccgccga tcaccacgtg gccggtcgcg tcggccagcg cgtccacgtc cagctcgaac
     5041 gcgtcctcga ggtagcggtc gatcagcacc gggctgtcgg gggcgtcggc cagggcgcgg
     5101 gtgacgtagc cgtcgagcgc cgcctcgtcg tagacgattg ccatgccgcg gcccccgagc
     5161 acgtacgagg ggcgcacgag cacggggaag ccgatcgacc cggcgatcgc gcgcgcctcc
     5221 tcgtgcgaga gcgcggtgcc gctggccggc tgcgcgatgc cgagccggtc gagcagggcg
     5281 gagaagcgct tgcggtcctc ggcgaggtcg atcgagtcgg ccgaggtgcc gatgatcggc
     5341 acgcccgccc ggtgcagcgg cagcgccagc ttgagcggcg tctgcccgcc gaactgcacc
     5401 aggcacgaca cctcgccgcc cgcctcgcgc tcgcgcgcga cgatccccat cacgttctcg
     5461 aaggtcaggg gctcgaagta cagccggtcg gccgtgtcgt agtcggtcga gacggtctcc
     5521 ggattgcagt tcaccatcac ggtctcgacc ccctcctcgc gcagggcgaa ggccgcgtgg
     5581 cagcagcagt agtcgaactc gattccctgg ccgatgcggt tgggccccga cccgaggatg
     5641 atcaccttgc ggcgcggcgt cgggtcggcc tcgcactcgg ccgcaaacgt gctgtagagg
     5701 tacggcgtga acgactcgaa ctccgcggcg caggtgtcga tccgcttgaa gacgcggcgg
     5761 atgccggccg cctcgccgag cgcctcgatg gccgggaccg cgacgcccga cagccggccg
     5821 atctcctcga ggctgaagcc cgcctgccgc gagg
//
LOCUS       DAOPQX010000221         5850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_14947_length_5850_cov_5.224159, whole genome shotgun sequence.
ACCESSION   DAOPQX010000221 DAOPQX010000000
VERSION     DAOPQX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14947_length_5850_cov_5.224159"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PKK95_11660"
     CDS             <1..616
                     /locus_tag="PKK95_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18920.1"
                     /translation="LVATLVEVEKRQLYRDAGCSSLYTFCTEILGLSESEAYLRMEAA
                     RAARKFPVILERLGDASLTLTAVSMLAKHLTEANHAALLAEARHKSKREIQGMLARIN
                     PRADAVAILRRLPAAPAVADRTRACAEDRTTVAPAPEESPRIFAETEPIPLQGGLGTT
                     AGRETKAKREAEEGLVSDLLAEHSRQQINVPGAPPSCVEELVLD"
     gene            complement(1723..2619)
                     /locus_tag="PKK95_11665"
     CDS             complement(1723..2619)
                     /locus_tag="PKK95_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC18921.1"
                     /translation="MRGGWGSGIQLEGGDDRMHEADFQAVSPGYFRTVGMALLGGREL
                     TAADRDGRQPVAVVNSEFARRFLPGADPLGRRLRRGSGPWLTIVGLVNDIRRQGKTGE
                     ITPQVYLSAAQTTLYPVRLSDLAVRASVEPRALLPSIQSAIWSIDKEQPVTNVRTLDE
                     IVDLSVRLRRFQMLLLTAFAGVALALSLVGISGVLTYAVSRRTPEFGIRLALGSPSGR
                     VLALVLRQAAVLIAAGLGVGVIAAWGLTRYLQSLLFEVRPHDVRIYASTAILLLVSAL
                     VASAIPARRAARVDPLTALRNE"
     gene            complement(2809..3144)
                     /locus_tag="PKK95_11670"
     CDS             complement(2809..3144)
                     /locus_tag="PKK95_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC18922.1"
                     /translation="MSRPPDLVQGTLDLLILKILALEPQNGWAISQRLRQVSGDVLQV
                     SDGSLYPALHKLEQEGWIASEWRASENNRRAKFYSLTRLGRRQLQREAANWDRLSAAI
                     SHVVKLGEA"
     gene            3378..4310
                     /locus_tag="PKK95_11675"
     CDS             3378..4310
                     /locus_tag="PKK95_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC18923.1"
                     /translation="MVRFDERHWALILGGSSGFGLASARKLAAEGLNVCVVHRDRRGA
                     MDRIEREFDAIRSHGRAFLSLNLDALSAEGMATVVASLGGRMGPEGRVRLLLHSIAFG
                     NLRPIVAAPPRADGSAALDRLAEALGLPGPDVERAVRRLAAEGEPAFQALDEVDYGEA
                     VLEDEDVARTIYAMGTSLLSWVQRLHAAGLFAPDARVIGLTSEGNEVAWRGYAAVAAA
                     KSALESVSRAIAVEFGPYGIRSNIVQPGVTDTPALRLIPGAARMKAGALRRNPLGRLT
                     TPDDVAGVICLLCSDEARWVNGAVIRVDGGEHVA"
     gene            4303..5319
                     /locus_tag="PKK95_11680"
     CDS             4303..5319
                     /locus_tag="PKK95_11680"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOC18924.1"
                     /translation="MHSRADSGDLYIHGVGHFHPENRIDNAFLSGLGIGTDDAWIMER
                     VGIRCRRTVLPLDYIASTRNARPAEAAAASLYTNAQTGAAAARLALARAGLQPSDIGL
                     VIAGGCSPQYSIPAEACLVAAELGIQAVAYDLNSACSSFAVQLHHLRGMRPEALPDYS
                     LVVNIENNTRTVDYNDRRTAVLWGDGSAAAVVSATVPAPVRVTHSMVQSDPAGWKKVV
                     IPAGGHFSQEGSAVQGFAIRKSVATLAALTPHLRHDPALVHFIGHQANLLMLNAVVER
                     AGLDPARHLKNVDEFGNCGAAGAPAVLSQHWDTLPSRCEIALVVVGSGLTWGGLVIEV
                     GRQG"
     gene            5316..>5850
                     /gene="fabA"
                     /locus_tag="PKK95_11685"
     CDS             5316..>5850
                     /gene="fabA"
                     /locus_tag="PKK95_11685"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF003509.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HOC18925.1"
                     /translation="MKYAEFLRRTAFSKEELLACAWGTLVEDPPAEGFSRLPGPPMLM
                     VDRVVEIAHRGPQGRIVAEYDVQVDAWFFQCHFRSDPVQPGCLGVDAIWQLIGFYGGV
                     RGAKGAGRALGAKEIEFFGQIRPYNKLVRYEIDIRRYTELAETGAAIAIGTGQVIVDG
                     EPIYTVKDAKVGMFKGIA"
BASE COUNT          951 a         1984 c         2070 g          845 t
ORIGIN      
        1 cctcgtcgca acgttggtcg aggtagagaa gcgccaactc tatcgcgacg ccggttgctc
       61 ctcgctctat acgttctgca ccgagatact cgggctgagt gagagcgagg cgtacctgcg
      121 gatggaggct gctcgtgcgg cgcggaagtt tccggtgatt ctcgaacgct tgggcgatgc
      181 ctcgctgacg ctgactgcgg tgagcatgct ggcgaagcac ctgaccgagg ccaaccacgc
      241 tgcgctgctc gcggaagctc gacacaagag caagcgcgag attcagggga tgctggcgag
      301 gatcaacccg agggccgacg cggtggcgat cctgcggagg ctgccggctg cgccggcagt
      361 ggcggaccgc acgagagcgt gcgccgagga tcgaaccacc gtggcccctg cccccgagga
      421 atcgccgagg atcttcgccg agactgagcc gatcccgctt cagggcggtc tcgggacaac
      481 agccggtcgc gagacaaagg ccaagcgcga ggcagaggag gggttggttt cagacctgct
      541 cgctgagcac tcgcggcaac agatcaacgt gcccggagcg ccgccgtcgt gcgtcgagga
      601 gctggtcctc gattagccac ggagccagag ccccgatgcg gtcgccagga ccggaacgcg
      661 ctcgggtccg cgctcggacg tcacacagct ctcgcctgag cgctaccggg tgcaggtcac
      721 gatcggcgcc gcgacccacg agaagctccg acgcgcgctg gacctgctca gtcatgtcgt
      781 tcccaacggc gatctcgaag aagtgttcaa ccgcgcgatc ggtctccttc tcgctgagct
      841 ggagaagacg cggctgggaa cgacctcgcg gcctcgtttg ccacgcctcg ccggcgagga
      901 tccgaaaggc aggacacgcg ccgaaggagc aagtgcgcca gacggtaacg cgcgacggga
      961 agaacccgta cgagcgaagg aaccggagcg gagaccggac gtgggtccaa ctgacgtccg
     1021 ctccagcaag cccggagcga agggccggac ggacgcgaag gcgagtagcg gacgaaggcg
     1081 ctccaggcac attccagcgg ccgtcaaacg ccaggtagtc gaacgtgacg gaatgaggtg
     1141 cacgttcgtc gggaacgacg ggcagcggtg ccgcgagacc cggatgctgg aattccatca
     1201 tcgcgatcga ttcgccgccg gcggaccgag catggtgtcc aacgtcgtgt tgacctgccg
     1261 acagcataac gccctcgagg cagagcggtt cttcggccga tcccacgttg cgaaggctcg
     1321 cgaagcgggc cgcgtagacg agacgccgca gcgggatcgc gatctgacaa cgccactatc
     1381 aactcgttcc aggacgacca gcgcggaccg aggcggatag cctttcaacg gaaccgtgat
     1441 tcgaacagtg acgagctggt tcatcaagcc gggcagcgcc caggccggcg ctgccgaagt
     1501 gccgccgccg tggacacgcg gatgggtgga gaacgcgccc tcaacgcgtc ctggaaatcg
     1561 tgcgggcaca gcccatcatc gtgttcatgc ggaagacgaa gggaccgagc gtcgcctcgc
     1621 tcggtctgca tggcacgacc gaaccggttc gcttgcgttg cccttcaacc gatcagcaag
     1681 gcaaccttct ggggcggaca gcacacagag tcagtccttg actcactcgt tgcgcaaggc
     1741 ggtcagcggg tccacgcgcg ccgcgcggcg ggctggaatc gccgacgcga ccagcgcgga
     1801 gacaaggagc aggatggccg tcgaggcata gatcctgacg tcgtgcggcc gcacttcgaa
     1861 gagcaggctc tgaaggtagc gggtcaggcc ccaggcggcg atgacgccca cgccgagccc
     1921 ggcggcgatg aggaccgccg cctgccgcag cacgagggca aggacccgcc ccgagggtga
     1981 cccgagcgcg agccggatgc cgaactcggg agtgcgccgc gacaccgcgt acgtcagcac
     2041 gccggaaatg ccgaccaggc tgagcgccag cgccacacct gcgaatgccg tcagcagcag
     2101 catctggaac cgtcgcagcc tgaccgacag gtcgacgatc tcgtcgagcg tccgcacgtt
     2161 ggtcaccggc tgctccttgt cgatcgacca gatcgcggac tggatcgagg gcagcagggc
     2221 tcgaggctcg accgatgctc gaacggccag gtccgacagc cgcaccgggt agagagtcgt
     2281 ctgtgccgcg gacaggtaca cctgcggcgt gatctctccc gtcttgccct gccgccggat
     2341 gtcgttgacg aggccgacga tcgtgagcca gggcccgctg ccccgccgca gccttcgacc
     2401 gagcggatcg gcgccgggca gaaaacgccg cgcgaactcg ctgttgacca ccgcgaccgg
     2461 ctggcggccg tcgcgatcgg cggcggtcag ctcccggccg cccagcagcg ccatcccgac
     2521 ggtgcgaaag tagcctgggc tgacggcctg gaagtcggcc tcgtgcatgc gatcgtcgcc
     2581 gccttccagt tggatgccgc tgccccagcc gccccgcatc ggcatcccat gtcgcgcagg
     2641 gccgcggccc gggcggcggc gggcgggagg ccgcggcgcc ggtaatcctc ggtcagttgc
     2701 tcgacgtgat atcgaagctc ctcttccagt tcgccctcga ccgtcccgcg gcgcaggagg
     2761 gatcgcaggc gaagcggaac cgtgtacagc cagcgttgcg gacgcatgtc atgcctcgcc
     2821 cagtttgacg acgtgggaaa tggcggcgga gagccggtcc cagttcgccg cttctctttg
     2881 cagctggcgc cggcccaggc gcgtgagcga gtagaacttc gcccggcggt tgttctcgct
     2941 ggcgcgccat tcggacgcaa tccagccttc ctgctcgagc ttgtgcagcg cgggatagag
     3001 cgaaccgtcg ctcacctgca ggacgtcgcc ggacacctgc ctgagccgct ggctgatggc
     3061 ccagccgttc tggggctcca gcgcaaggat cttcaggatg aggaggtcga gggtcccctg
     3121 cacgaggtct ggcggtcggc tcacgcgcac tcctctcggt aaccgaggac agcattatcc
     3181 gctgcttcgc ctcggtaagc gctccccaca tctccttctc cattcccggt tcgtctgcgt
     3241 taagatgtcc gctactccgg acagcggttc agccaggtcc ccggcggcac gggcgcctcc
     3301 ggccgccgcc ggtgtgccgc cgcgggcccg gcagcccggg cgtggaaggc agtgggggac
     3361 gaggagcgct gcgcgagatg gtgcggttcg acgaacgaca ttgggccctg atcctgggag
     3421 gctccagtgg tttcggcttg gcctcggcga ggaagctcgc cgccgagggt ctcaacgtgt
     3481 gcgtggtgca ccgcgaccgc cggggcgcga tggaccgcat cgagcgcgag ttcgacgcga
     3541 tccgctcgca cgggcgcgcc ttcctgagcc tcaacctcga cgccctgagc gccgagggca
     3601 tggcgacggt ggtcgcgtcg ctgggcgggc ggatgggccc ggaaggacgc gtgcggctgc
     3661 tgctgcactc gatcgcgttc ggcaacctgc gcccgatcgt cgcggcgccg ccaagggccg
     3721 acgggtctgc cgcgctcgac cggctggcgg aagccctggg cctgccaggg cccgacgtgg
     3781 agcgcgcggt gagacgactc gccgccgagg gcgagcccgc gttccaggcg ctcgacgagg
     3841 tggactacgg cgaggccgtc ctcgaggacg aggacgtggc tcgcaccatc tacgcgatgg
     3901 gcaccagcct cctctcgtgg gtgcagcgcc tgcacgcggc cggcctcttc gctcccgacg
     3961 cgcgggtgat cggcctcacc agcgagggca acgaagtggc ctggcgcggg tacgccgcgg
     4021 tggccgccgc caagtcggcg ctcgaatcgg tttcgcgcgc catcgccgtc gagttcggcc
     4081 cctacggcat ccggtcgaac atcgtccagc caggggtcac cgacacgcct gcgctccggc
     4141 tgatcccggg cgccgcccgc atgaaggcgg gcgccctgcg gcgcaacccg ctcgggcgcc
     4201 tgacgacccc cgacgacgtc gccggcgtca tctgcctgct ctgttccgac gaggcgcggt
     4261 gggtgaacgg ggcggtgatc agggtcgacg gaggggaaca cgttgcatag ccgcgccgac
     4321 tcaggcgatc tctacattca cggcgtcggc cactttcacc cggagaaccg catcgacaac
     4381 gcgttcctgt cggggctggg catcggcacc gacgacgcgt ggatcatgga gcgggtgggc
     4441 atccgctgcc ggcggacggt gctgccgctc gactacatcg cgtccacgag gaatgcgcgc
     4501 ccggccgagg ccgccgcggc ctccctctac acgaacgccc agaccggcgc cgcggccgcc
     4561 cgcctcgcgc tggcccgcgc cggcctgcag ccctcggaca tcggcctcgt gatcgccggc
     4621 ggctgctcgc cccagtactc aatcccggcc gaggcctgcc tcgtcgccgc ggaactgggc
     4681 atccaggcgg tcgcctacga tctcaactcg gcctgctcga gcttcgcggt gcagctccac
     4741 cacctgcgcg ggatgcggcc cgaggccctg cccgactact cgctggtggt gaacatcgag
     4801 aacaacacgc gaaccgtgga ctacaacgac cgccgcacgg cggtgttgtg gggtgacgga
     4861 agtgccgcgg cggtcgtgtc cgcgacggtg cccgcgccgg tgcgcgtcac gcacagcatg
     4921 gtgcagtccg acccggccgg ctggaagaag gtcgtcatcc cggccggcgg gcacttctcg
     4981 caggagggct cggccgtcca ggggttcgcc atcaggaagt cggtcgccac gctggccgcg
     5041 ctgacgcccc acctccggca cgatcccgcg ctcgtgcact tcatcgggca ccaggcgaac
     5101 ctgctgatgc tcaacgcggt cgtggagcgg gccgggctcg acccggcgcg tcacctcaag
     5161 aacgtggacg agttcggcaa ttgcggcgcc gccggcgcac cggccgtgct ctcgcagcac
     5221 tgggacacgc tgcccagccg ctgcgagatc gcgctcgtcg tcgtcggatc cggcctcacg
     5281 tggggcggcc tggtgatcga ggtggggagg cagggatgaa gtacgcggag ttcctgcggc
     5341 gaacggcatt ctccaaggag gagctgctcg cgtgcgcgtg ggggacgctg gtggaggacc
     5401 cgccggcaga aggcttctcg aggctgccgg gcccgcccat gctcatggtg gaccgcgtgg
     5461 tggagatcgc ccaccgcggg ccgcagggcc gcatcgtggc ggagtacgac gtccaggtgg
     5521 acgcctggtt cttccagtgc cacttccgct ccgaccccgt gcagcccggg tgcctgggcg
     5581 tggacgccat ctggcagctc atcgggttct acggcggcgt gcgcggcgcc aagggggcgg
     5641 gccgggcgct cggggcgaag gagatcgagt tcttcgggca gatccgcccc tacaacaagc
     5701 tggtccgcta cgagatcgac atccggcgct acaccgaact ggcagagacc ggcgcggcca
     5761 tcgcgatcgg gacgggccag gtgatcgtgg acggggaacc gatctacacg gtgaaggacg
     5821 cgaaggtcgg aatgttcaag gggatcgcgt
//
LOCUS       DAOPQX010000222         5779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15186_length_5779_cov_4.365828, whole genome shotgun sequence.
ACCESSION   DAOPQX010000222 DAOPQX010000000
VERSION     DAOPQX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15186_length_5779_cov_4.365828"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1130)
                     /locus_tag="PKK95_11690"
     CDS             complement(48..1130)
                     /locus_tag="PKK95_11690"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOC18926.1"
                     /translation="MRSKVAALRVEPATILESIERLCELGGMRAALSPAAPTILKDNI
                     SWHFPFPGANTTPWQLEGTIRALRGGGFGDLTCVQNKTVVTDAFKGEDLNKYVPILQR
                     HGIPILYNFRESDMKWVRYAPKARMHVLPRIFPDGIHVPDFFFGKNIVHLPTVKCHIY
                     TTTTGAMKNAFGGLLHTHRHYTHSWIHRTLVDLLAIQKEIHTGIFAVMDGTTAGNGPG
                     PRTMYPVVKNVVLASADQVAIDAVAAKLMGFDPLSIEYIRVAHEDGLGVGDPRDIELV
                     GDADLASESWGFQVGDNLASLGGDLFWFSPLKVFQKLLFHTPVVHLFVFASEAYHDYY
                     RWPVKDRRTFEHWLKHTAWGKLFEQY"
     gene            complement(1201..1938)
                     /locus_tag="PKK95_11695"
     CDS             complement(1201..1938)
                     /locus_tag="PKK95_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC18927.1"
                     /translation="MSVSERVRVAVVARWPVVAAGIRELLGPHEDLEVIAAFESLGEA
                     IAGCGRSDVVLLDPDSEEISLRAVPALAQAGSGRILVFTAAADAAVHTRAIELGASGV
                     VSKEEAAPALARAIVKVHAGELWLARTRAGVALSIARRRDRDPEAFKIESLTRREREI
                     VGLVGEGLRNRSIAERLFISEATVRNHLTSILSKLDLSDRFDLAVYAFRHDLVPELQV
                     VPRNGVGLFLPPAHQPMSAAARQGFAG"
     gene            complement(2406..3656)
                     /locus_tag="PKK95_11700"
     CDS             complement(2406..3656)
                     /locus_tag="PKK95_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOC18928.1"
                     /translation="MSTRWDHRFAQRTQRMTSSAIRELLKLTAQPDIISFAGGLPGPA
                     LFPIEEVREAADTVLQQHGRKALQYSTTEGYPPLREMICRHMARYGIVVDINNVLITS
                     GSQQALDLIGKVLINPGDKVLTENPTYLGALQAFTMYSADFITVPMDDDGLQVDKLEE
                     ALRSGPKFMYLLPNFQNPSGVTMSVERRREVIRLSDHYGIPIIEDDPYGQLRYEGDHL
                     ESLVVGDARWRDCHTHGKFTGNVIYLSTFSKTLAPGLRMAWVVAPEEVIHRLVQAKQG
                     TDLQCSTFDQMVTYEVGRGGFLDRHVHRIREVYGQQRLRMIEALEKAFPDPSLGVRWT
                     RPQGGLFLWVTLPEGIDTGEVLLEAVKEKVAFVPGAPFHPGGDGRNSMRLNFSNASTE
                     QIGEGIERLGRVVTRLLERKPAVA"
     gene            3767..4570
                     /locus_tag="PKK95_11705"
     CDS             3767..4570
                     /locus_tag="PKK95_11705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC18929.1"
                     /translation="MTDIPSSKRPAGAEGWQGWDDYAPFYDWENARTMGRQDVRFWQD
                     LARREGGPVLELGSGTGRLTIPVSRTGVRVVGVDRSRGMLAFARRRARRLPAAGRPRF
                     ALGDIRALPFAARTFQALMAPYGMLQSLTSDADLEATLAGARRVLVPGGVLGVDLVPD
                     LPAWDEYDRRIRFRGTSRAGARVTLIESVRQDRRRGLTVFDQEYVEHRGRQTATHRFS
                     LTFRTLPLAEMLARIETAGFGVTALLGDYRGRAWDIRADVWLILASKRR"
     gene            4701..5450
                     /locus_tag="PKK95_11710"
     CDS             4701..5450
                     /locus_tag="PKK95_11710"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOC18930.1"
                     /translation="MSGHSKWHTIKHKKGAADAKRGRIFTRIIKEITVAARAGGGDPD
                     SNPRLRTIVAEAKSVNMPAENIKRAIRRGTGEEPGVNYEEVTYEAYGPGGTAIILEGL
                     TDNKNRTVGELRHLLDKYNGKMADRNTALRKFSKRGQIVIEKGKVDEDTLMAAVLDAG
                     ADDMRDDEESWEILTAPEAFDAVREAVRALEVEPVSAAVAMVPFEYVKLTGKEAQSML
                     KLMDVLEDHDDVQHVWSNFDVDEKEIEASLS"
     gene            5447..>5779
                     /locus_tag="PKK95_11715"
     CDS             5447..>5779
                     /locus_tag="PKK95_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOC18931.1"
                     /translation="MRIFGIDPGSERTGYGCVETDGSRHRLVVCGAIAVPPRCGFPAQ
                     LHAIHQRLAALLAEHRPECVAIESVFHAVNARSALKLGQARGVAVLAAVEGGYPVFEY
                     APAEVKRAV"
BASE COUNT          903 a         1927 c         2065 g          884 t
ORIGIN      
        1 ccagaaactc gcgaatatcg gggtgatcca cggggacggc ctttccgtca atactgctcg
       61 aacagctttc cccaggcggt gtgcttcagc cagtgctcga aggtgcgccg gtccttcacg
      121 ggccagcggt agtagtcgtg gtacgcctcg gaggcgaaga cgaagaggtg cacgacgggc
      181 gtgtggaaga ggagcttctg aaagaccttc agcggcgaga accagaacag gtcgccgccc
      241 aggctcgcca ggttgtcgcc aacctggaag ccccacgact cggaggccag gtcggcgtca
      301 cccaccagct cgatgtcgcg cgggtcgccg acgccaaggc cgtcctcgtg cgcgacgcgg
      361 atgtactcga tcgacagcgg gtcgaatccc atcagcttcg ccgcgaccgc gtcgatggcg
      421 acctggtcgg ccgacgccag cacgacgttc ttcacgaccg gatacatggt gcgcgggccg
      481 gggccgttgc cggccgtcgt cccgtccatc accgcgaaga tcccggtgtg gatctccttc
      541 tggatggcga gcaggtcgac cagcgtgcgg tggatccacg agtgggtgta gtgccggtgc
      601 gtgtggagca ggccgccgaa cgcgttcttc atcgcgccgg tggtggtggt gtagatgtgg
      661 cacttcaccg tcggcaggtg gacgatgttc ttgccgaaga agaagtcggg tacgtggatg
      721 ccgtcgggga agattctggg gaggacgtgc atgcgggcct tcggcgcgta ccggacccac
      781 ttcatgtcgc tctcgcggaa gttgtagagg atggggatcc cgtggcgctg caggatcggg
      841 acgtacttgt tcaggtcctc gcccttgaag gcgtcggtga ccaccgtctt gttctgcacg
      901 caggtgaggt cgccgaaacc gccgccccgg agcgcccgga tcgttccctc gagctgccac
      961 ggcgtggtgt tcgcccccgg gaaggggaag tgccacgaga tgttgtcctt gaggatggtg
     1021 ggcgcggccg gggacagggc ggcgcgcatg ccccccaact cgcacaggcg ctcgatggac
     1081 tccaggatcg tggcgggttc gacccgcagc gcggcaacct tgctcctcaa cggcctccct
     1141 ctcggtaacc tcccattcta gcaggccgtg gggaaaggcc cacggcctgc tggctggaac
     1201 tcacccggcg aagccctggc gcgctgccgc cgacatcggc tggtgtgcgg gcggaaggaa
     1261 cagacccacg ccgtttcggg gcacgacctg gagttccggg accaggtcgt ggcggaacgc
     1321 atacaccgca aggtcgaagc ggtcggagag atcgagcttg ctgagaatgg acgtcaggtg
     1381 gttgcggacc gtcgcctcgc tgatgaagag gcgctcggcg atcgaacggt tgcgcagccc
     1441 ctcgccgacc agccccacaa tctcgcgctc gcgccgcgtc aggctctcga tcttgaaagc
     1501 ctcgggatcc ctgtcccgcc tcctcgcgat cgagagcgcg acccccgcgc gggttcgagc
     1561 cagccaaagc tctccggcgt gcaccttcac gatcgcgcgg gccagcgccg gggcggcttc
     1621 ctccttcgac accacgcccg aggcccccag ctcgatcgcc cgcgtgtgaa cggcggcgtc
     1681 ggcggccgcc gtgaagacga ggatccggcc gctgcccgcc tgcgcgaggg ccggaaccgc
     1741 ccgcagggaa atctcctccg agtcggggtc caggagaacg acgtcgctgc gcccgcagcc
     1801 ggcgatcgcc tcgcccagcg actcgaaggc agcaatcacc tccaggtcct cgtgaggccc
     1861 gagcagctcc ctgattccag cggccacgac cggccaccgg gccactaccg ccacgcgcac
     1921 ccgctccgat acggacatgg tgattccccc cgatcaggcc ccgcgcgacc ccggtgcacc
     1981 agcaccgccg gtcgcgtcga acgtcatggc cggcgaaata gcagcttccg cgccacgcgg
     2041 cgatcggtcg ggcgccgggc tcgccggagc tggttggagg cgggtccggg gggcaccccg
     2101 ggcagggagc tggaaggcct gcccgtcacc ccgcccatcg gcgagaacgg gggaggcggg
     2161 agcgatagtg gaccgattac atccaaaaac gcgacgcgtg ccgccggcac cgttcggccg
     2221 cctggtctcg tgccgggaat cgggaggccc ggccgggggg gccgggccag cgctgcgcga
     2281 ggtgcttgcc gggagctgtt ttcgagttgc cgcggcgagg acatatccga ctcatcgggt
     2341 cgccatgtcc tcgacttcag gaaaaaaagt cagcccgaag atgaacttcc gaagctcggg
     2401 tgcggtcaag ccaccgcggg cttcctctcg agcaggcggg tcaccacgcg gccgagccgc
     2461 tcgattccct cgccgatctg ctcggtgctc gcgttgctga agttgagccg catgctgttc
     2521 cgcccgtcgc cgcccgggtg gaaaggcgcc ccgggaacaa acgccacctt ctctttgacc
     2581 gcctcgagca gcacctcgcc ggtgtcgatc ccctcgggca gagtgaccca caggaacaac
     2641 ccgccctggg gccgcgtcca ccgcacgccg agcgacggat cggggaaggc cttctcgagc
     2701 gcctcgatca tccgcaggcg ctgctggccg tacacctcgc ggatgcggtg gacgtggcgg
     2761 tcgaggaacc cgccgcgccc cacctcgtag gtgaccatct ggtcgaaggt gctgcactgc
     2821 aggtcggtgc cctgcttggc ctgcacgagg cgatggatga cctcttcggg ggcgaccacc
     2881 cacgccatgc gcaggccggg cgccagggtc ttgctgaacg tgctcaggta gatcacgttg
     2941 ccggtgaact tcccgtgggt gtggcagtcg cgccagcggg cgtcgccgac gaccagcgac
     3001 tcgaggtgat cgccctcgta ccgcagctgc ccgtaagggt cgtcctcgat gatcgggatc
     3061 ccgtagtgat cggagaggcg gatcacctcc cgccgccgct cgacgctcat ggtgacgccg
     3121 gagggattct ggaagttcgg cagcaggtac atgaacttcg ggcccgaccg aagcgcctcc
     3181 tccagcttgt ccacctgcag gccgtcgtcg tccatgggga cggtgatgaa gtcggccgag
     3241 tacatcgtga acgcctgcag ggcgccgagg taggtcgggt tctcggtgag gaccttgtcg
     3301 cccgggttga tcaggacctt gccgatcagg tcgagcgcct gctgggatcc gctggtgatg
     3361 aggacgttgt tgatgtccac cacgatgccg tagcgcgcca tgtggcggca gatcatctcg
     3421 cgcagcggcg ggtatccctc ggtggtgctg tactggaggg ccttgcggcc gtgctgctgg
     3481 agaaccgtgt cggcggcttc gcgaacctct tcgatcggaa agagggcggg gcccgggagc
     3541 ccgccggcga acgagatgat gtcgggctgc gccgtcagct tgagcagctc gcggatggcc
     3601 gaactggtca tgcgctgcgt gcgctgtgcg aagcggtggt cccaccgggt gctcatggtc
     3661 acgcggctcc tcccgtgatg gcggccgggc gtccggcacc ccgatactac cccggacgtt
     3721 gggcgattgc caggaaccct gtatcatcct cgggcgcgag gcgcacatga ccgacatccc
     3781 ctcgagcaag cgcccggcgg gcgccgaagg ctggcagggc tgggacgact acgcgccgtt
     3841 ctacgactgg gagaacgccc gcaccatggg gcgccaggac gtccgcttct ggcaggatct
     3901 cgcccgccgc gagggggggc cggttctcga gttggggagc gggacgggcc ggctgacgat
     3961 ccccgtgtcg cgaaccggcg tcagggtcgt cggcgtcgac cgctcgcggg ggatgctggc
     4021 cttcgcgcgc cggcgggccc gccggttgcc ggcggccggg cggccgcgct tcgcgctggg
     4081 cgacatccgg gcacttccct tcgccgcccg caccttccag gcgttgatgg ccccgtacgg
     4141 gatgctgcag tcgttgacca gcgacgccga cctggaggcg acgctggccg gtgcgcggcg
     4201 tgtgctggtg cccggcgggg tgctgggcgt cgacctcgtg cccgacctgc cggcctggga
     4261 cgagtacgac cgtcgcatcc ggttccgggg caccagtcgg gccggcgcgc gggtcacgct
     4321 catcgaatcg gtccggcagg atcggcgccg cggcctgacg gtcttcgacc aggagtacgt
     4381 ggagcaccgg gggaggcaaa ccgcgacgca ccgcttctcc ctcacgttcc gcacgctccc
     4441 gctggccgag atgctggccc ggatcgaaac ggccgggttc ggggtgacgg ccctcctggg
     4501 agactaccgt ggaagggcct gggacatccg ggccgacgtc tggctgatcc tggcgtcgaa
     4561 gcgccgctga gggcgtcgcg cggccggcga gcgggcccgg cggggaggcc gcccgcggac
     4621 aggccgggac ggctgtccgg ccgcgcggag tctgtggtac aatcttgccg cttttccatt
     4681 gatttagctg gagtttcccg atgtctggcc attccaagtg gcacacgatc aagcacaaga
     4741 agggcgccgc cgacgccaag cgcgggcgca tcttcacccg gatcatcaaa gagattacgg
     4801 tggccgcccg cgccgggggc ggcgaccccg attcgaaccc gcggctgcgc acgatcgtgg
     4861 ccgaggcgaa gtcggtcaac atgcccgccg agaacatcaa gcgggcgatc cggcgcggga
     4921 ccggcgagga gcccggcgtc aactacgagg aggtcaccta cgaggcctac ggtcccgggg
     4981 gaacggcgat catcctcgag gggctgaccg acaacaagaa ccgcaccgtc ggcgagcttc
     5041 gacacctgct cgacaagtac aacggcaaga tggccgatcg gaacaccgcc ctgcgcaagt
     5101 tcagcaagcg gggccagatc gtgatcgaga aggggaaggt ggacgaggac acgctgatgg
     5161 ccgcggtcct cgacgccggc gccgacgaca tgcgcgacga cgaggagagc tgggagatcc
     5221 tgaccgcccc cgaggcgttc gacgcggtgc gcgaggccgt gcgggcgctc gaggtcgagc
     5281 cggtgagcgc ggcggtggcg atggtgccct tcgagtacgt caagctgacg ggcaaggaag
     5341 cgcagtcgat gctcaagctg atggacgtcc tcgaggacca cgacgacgtg cagcacgtgt
     5401 ggtcgaactt cgacgtggac gagaaggaga tcgaggcctc gctctcgtga ggatcttcgg
     5461 catcgacccc ggctccgagc gcaccggcta cgggtgcgtg gagaccgacg ggagccgcca
     5521 ccggctcgtg gtctgcgggg cgattgccgt gcccccccgt tgcgggtttc ccgcgcagct
     5581 tcacgccatc caccagcggc tggcggcgct gctggccgag caccgccccg agtgcgtcgc
     5641 gatcgagagc gtcttccacg ccgtcaacgc ccgcagcgcg ctgaagctgg ggcaggcccg
     5701 cggcgtcgcg gtgctcgcgg cggtcgaggg cggctacccc gtgttcgagt acgcccccgc
     5761 cgaagtgaag cgcgcggtc
//
LOCUS       DAOPQX010000223         5760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15256_length_5760_cov_3.785101, whole genome shotgun sequence.
ACCESSION   DAOPQX010000223 DAOPQX010000000
VERSION     DAOPQX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15256_length_5760_cov_3.785101"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PKK95_11720"
     CDS             <1..419
                     /locus_tag="PKK95_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18932.1"
                     /translation="AADRLLDELPAAGIEQVIVVSAFPEVEGPHRLHRARGDWRGRLG
                     EFLAGAETASLRGLLARDRGFRATFLIRPAYNPIGPLDLAGCYDERSDRHVAPGELVD
                     RGYEDAYRQFIDPVVGAGGERLEEVAAPAGRGAAGD"
     gene            421..1593
                     /locus_tag="PKK95_11725"
     CDS             421..1593
                     /locus_tag="PKK95_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC18933.1"
                     /translation="MTRRGVSTELIHQGEDRPPRADPVNVPIYETTTFLFESADEVRR
                     YQEGRAEKYLYSRYANPTVVVTEQKLAALDAADAALVFASGMAAASTILLGLLNPGDE
                     LLCSSALYGGTFHLINDVVRRFGVAVRFATLEELREPGRLIGDRTRVCWVESPINPTL
                     RCVDVRRVAAACRSRGVISVVDNTFASPVNQRPLALGVDLAMQSATKYLNGHSDVTGG
                     VVSGSTALVKRLDLTRRFLGGVLDPLAAYNLARGLKTLPVRMACHNESALAIARFLEG
                     HAAVRRVYYPGLPSHPDHEIARSQMSGYGGMVCFEVEGGLEGASRVFDRLGLIRRATS
                     LGSVDSLASLPVLTSHWNYSDEELERAGVTPGMVRLSVGLEDEEDLVADLEQALAG"
     gene            complement(1601..3031)
                     /locus_tag="PKK95_11730"
     CDS             complement(1601..3031)
                     /locus_tag="PKK95_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOC18934.1"
                     /translation="MKCLVVCQTEIVARMLQTALQPGFPVQYLVESRTLARRLHDGGL
                     DVIAGDPRRTDTYLKVDLDPTTCVLLEDALRPGLRRTIAAIRDAGGTLVYVLATNTAA
                     ARREDEVRAAFPEVTYLSLAELFGGPLLTQVGRSFTRARVQQYQRYFADADRVLILLH
                     NDPDPDAMASGLALRNILRRTKATAILGTLQGMTRPENLRMANMLDIQVESMTPAALA
                     EFDRVATVDVQPHYFGEMLDHVDLVIDHHPPQPGYSAVFRDIRPEYGSTSTIMTEHLR
                     AVDVNISERTATALLYAIKSDTLFFNRQATREDLEAFSFLYSLADATLIRKMEGSEIT
                     LERLRHVVKALEAGRLDRQVFCSFMGETPREDLIAYIADFFLELEDVKWTIVAGVVNQ
                     MLVMSVRNLGYTRNAGEFVRKYFADLGSAGGHRSMAKAVVPLDGFRAKFKVEDAQGIV
                     DKVHDLAVQFLREHQAPERRKEAVKG"
     gene            3173..4009
                     /locus_tag="PKK95_11735"
     CDS             3173..4009
                     /locus_tag="PKK95_11735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC18935.1"
                     /translation="MKSGLIVALAAAGAIVSAQGRQTVFRSGVDLVTFGVTVVDAKNA
                     VVTGLNAEDFELYEDGKKQSLLYFMPGDADASDLPQSALHLGVVFDISGSMQDDLTFA
                     RSAAIKFLGALHEARDYTLVEFDTEIRVARFSQAEFPRLVERIRTRRADGYTALWDAT
                     GVYLDGASGQEGRKVLILYTDGADNASNIRFNELLDLVKASDVTVHAIGFLEHLRSGD
                     RLESRLRMQQVAEQTGGQAFFPTSLKELDSVYERVAAEIAGQYTLGYISTNPRCISPP
                     HG"
     assembly_gap    4017..4026
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4208..>5760
                     /gene="uvrB"
                     /locus_tag="PKK95_11740"
     CDS             4208..>5760
                     /gene="uvrB"
                     /locus_tag="PKK95_11740"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOC18936.1"
                     /translation="MPDRFTLVSDFELRGDQAGAIAELEQGLARGDRHQVLLGVTGSG
                     KTFTMAQVMARANRPALVMVHNKTLAAQLYQEFRRFFPHNAVEYFVSYYDYYQPEAYV
                     PASDTYIEKEATINDEIDRMRLSATRSLFERRDVLIVASVSCIYGLGSPEAYYGMMLP
                     LEIGQRIEREQILRKLVDIQYERNDVEFGRGTFRVRGDIIEVYPSYEEQAVRIGLFGD
                     EVDDLVVFDPLTGRTSRRHDRIAIYPKSHFATPRERTRQAIASIKAELAERRAQLESE
                     GKLLEAQRLHQRTMFDLEMIREIGYCHGIENYARHLTGRAPGQPPPTLLDYLPPDAVV
                     FVDESHQTVPQIRGMYHGDRSRKEVLVEYGFRLPSALDNRPLNFEEWESRTHQVVFVS
                     ATPGPYELRCSGGVVVEQIIRPTGLTDPPIEVRPVKGQVDDLLGEIRRRAESRERVLV
                     TTLTKRMAEDLTAYYQELGVRVRYLHSDIDTLERVEILRDLRRGEFDVLVGINLLREG
                     LDLPEVSLVA"
BASE COUNT          875 a         1891 c         2094 g          890 t
ORIGIN      
        1 gagccgccga tcggctcctc gacgagctgc cggccgccgg catcgaacag gtgattgtgg
       61 tgtcggcctt cccagaggtg gagggaccgc accggctgca ccgcgcccgc ggcgattggc
      121 gagggcggct gggggagttc ctggccgggg ccgagacagc ttccttgcgc gggctgctcg
      181 cgcgcgaccg gggctttcgt gccaccttcc tgatccggcc cgcgtacaac ccgatcggcc
      241 ccctcgatct ggccggctgc tacgacgagc ggtccgaccg gcacgtcgcg cccggcgagc
      301 tggtggaccg cgggtacgag gacgcctacc gccagttcat cgacccggtg gtcggggccg
      361 gcggcgagcg cctcgaggag gttgccgcgc cggcgggccg cggcgccgcg ggggactgag
      421 atgacacgac gaggggtctc gaccgagctg attcaccagg gggaggaccg gcccccgcgc
      481 gccgatccgg tcaacgtccc gatctacgag acgacgacct tcctgttcga gtcggccgac
      541 gaggtgcgcc gctaccagga aggacgggcg gagaagtacc tctactcgcg ctacgccaat
      601 ccgacggtcg tggtgaccga gcagaagctg gcggcgctcg acgctgccga cgccgcgctg
      661 gtctttgcct cggggatggc ggccgcgtcg acgatcctgc tcgggcttct gaacccgggc
      721 gacgagctgc tctgcagttc cgcgctctac ggcggcacct ttcacctcat caacgacgtc
      781 gtccggcggt tcggggtggc ggtgcgcttc gcgaccctgg aagaacttcg cgagcccggg
      841 cgcctgatcg gcgatcgaac gcgcgtctgc tgggtggagt cgccgatcaa cccgaccctg
      901 cggtgcgtgg acgtgcgccg ggtggccgcg gcctgccgtt cccgcggggt gatctcggtg
      961 gtcgacaaca ccttcgcgag ccccgtgaac cagcgtccgc tggcgctcgg cgtggacctg
     1021 gcgatgcaga gcgccacgaa gtacctgaac ggccactccg acgtgacggg gggcgtggtg
     1081 agcggcagca ccgcgctcgt caagcggctc gacctgacgc ggcggttcct cggcggcgtg
     1141 ctcgacccgc tggcggccta caacctggcc cggggcctca agaccctgcc ggtgcgaatg
     1201 gcctgccaca acgagtcggc gctcgcgatc gcccgcttcc tggaaggcca cgccgcggtg
     1261 cgccgggtct attatccggg cctgccgtcg caccccgacc acgagatcgc ccggtcgcag
     1321 atgagcggct acgggggcat ggtgtgcttc gaggtggagg gcggactgga gggggcttcg
     1381 cgggtgttcg atcgcctcgg cctgattcgc cgcgccacca gcctcggcag cgtcgacagc
     1441 ctggcgagcc tgccggtcct cacctcgcac tggaactaca gcgacgagga actggagcgg
     1501 gcgggcgtca cgccggggat ggtgcgcctc tcggtcgggc tcgaggacga ggaggacctg
     1561 gtcgcggacc tcgagcaagc gctggcggga tgagccggct tcagcccttg acggcttcct
     1621 tccggcgctc gggggcctgg tgctcgcgga ggaactgcac cgcgaggtcg tgcaccttgt
     1681 cgacgatgcc ctgggcgtcc tcgaccttga acttcgcgcg gaagccgtcg agcggcacga
     1741 ccgccttggc catcgaccgg tggccgccgg cgctcccgag gtcggcgaag tacttgcgga
     1801 cgaactctcc ggcgtttctc gtgtagccga ggttgcggac cgacatcacc agcatctggt
     1861 tcacgacgcc ggcgacgatc gtccacttga cgtcctcgag ttcgaggaag aagtcggcga
     1921 tgtacgcgat cagatcctcg cggggcgtct ctcccatgaa ggagcagaag acctgccggt
     1981 cgaggcgccc ggcctcgagc gccttgacga cgtggcgaag gcgttcgagg gtgatctccg
     2041 agccctccat cttgcggatc agcgtcgcgt cggcgagcga gtagaggaac gagaaggcct
     2101 cgaggtcttc ccgcgtcgcc tggcggttga agaacagggt atccgacttg atcgcgtaga
     2161 gcagggccgt cgccgtccgc tcggagatgt tgacgtcgac ggcgcgcagg tgctcggtca
     2221 tgatcgtgga ggtggagccg tattccggcc ggatgtcgcg gaagaccgcg ctgtaacccg
     2281 gctgcggcgg gtggtggtcg atgaccaggt cgacgtgatc gagcatctcg ccgaagtagt
     2341 gcggctgcac gtccacggtc gccacgcggt cgaactcggc cagcgcggcg ggggtcatcg
     2401 actcgacctg gatgtcgagc atgttcgcca tccggaggtt ctcggggcgc gtcatcccct
     2461 gtagcgtgcc gaggatggcc gtggccttgg tgcggcggag gatgttccgg agcgccagcc
     2521 cgctggccat cgcgtcggga tccgggtcgt tgtggagcag gatcaggacg cggtccgcgt
     2581 ccgcgaagta gcgctggtac tgctgcaccc gggcgcgcgt gaacgagcgg ccgacctgtg
     2641 tgagcagcgg gccgccgaac agctcggcca gcgacaggta ggtgacctcg gggaaggcgg
     2701 cgcgcacctc gtcctcccgt cgggcggcgg ccgtgttggt ggcgaggacg tagacgagcg
     2761 tgccgccggc gtcgcggatg gcggcgatcg tccgcctcag gcccggccgg agggcgtcct
     2821 cgagcagcac acaggtcgtg gggtcgaggt ccaccttgag gtaggtgtcg gtccggcggg
     2881 gatcgccggc gatcacgtcg agcccgccgt catggaggcg ccgggcgaga gtgcggctct
     2941 cgacgagata ctggaccggg aaccccggtt gcagcgccgt ctggagcatg cgggccacga
     3001 tctcggtctg gcagacgacc aggcacttca tcggcaggcc caaggagcgc gcggtcccga
     3061 gagagggaaa tccatcgccg caacctttta ttatacgcgt gtgggtgggt tttcggggtc
     3121 gggccacgcc gccgtgaacg gctgacgtag atcaaggcac cggggggaga ccgtgaaatc
     3181 agggctgatt gtcgcgctgg cggccgccgg cgcgatcgtg tcggcgcagg gccgccagac
     3241 ggtgttccgg agtggtgtgg atctcgtcac atttggcgtc acggtcgtgg acgccaagaa
     3301 cgcggtcgtg acgggcctga acgccgagga cttcgagctg tacgaggacg gaaagaagca
     3361 gtcgctgctc tacttcatgc cgggggacgc cgacgcctcc gacctgccgc agtcggccct
     3421 gcacctcggc gtggtcttcg acatcagcgg ctcgatgcag gacgacctga cgttcgcccg
     3481 gagcgcggcc atcaagtttc tcggcgcgct ccacgaggcg cgggactaca cgctggtgga
     3541 gttcgacacc gagatccgcg tcgcccggtt cagccaggcc gagttccccc ggctcgtgga
     3601 gcgcatccgc acccggcgcg cggacggcta caccgcgctg tgggacgcca cgggcgtgta
     3661 cctcgacggg gcctcggggc aggaggggcg gaaggttctc atcctgtaca ccgacggcgc
     3721 ggacaacgcg agcaacatcc gcttcaacga gctgctcgac ctcgtgaagg cgtcggacgt
     3781 gacggtccac gcgattggct tcctcgagca tctccggtcg ggcgatcgtc tggagtcgcg
     3841 cctgcggatg cagcaggtgg ccgagcagac cgggggacag gcgttctttc ccacctcgtt
     3901 gaaggagctc gattcggtgt acgagcgcgt ggcggcggaa attgccgggc agtacactct
     3961 agggtacatc tcgaccaacc cgcggtgcat ctcgccgccg cacgggtaga aacgctnnnn
     4021 nnnnnnacca acccgcaggc cgacgggacg tggcgcaagg tcgaggtcag gctggcgggc
     4081 ggtcgagggc cgaagggggc gaaggtgcgg acccgggggg gctactacgc cccgctcaag
     4141 caggacgccc gcgactgagg gcgcgccgct tggtgttcgc cgccctttcg cgtacgatcg
     4201 tacggttgtg cccgaccgct tcaccctcgt ctcggacttc gagctgcgcg gggaccaggc
     4261 cggcgcgatt gccgagctgg agcagggcct cgcgcgcggc gaccgccacc aggttctgct
     4321 gggggtaacg gggtccggca agaccttcac catggcccag gtgatggcgc gggccaaccg
     4381 cccggccctc gtcatggtgc acaacaagac gctggccgcg cagctctacc aggagttccg
     4441 ccgcttcttc cctcacaacg cggtcgagta cttcgtcagc tactacgact actaccagcc
     4501 cgaggcctac gttccggcca gcgacaccta catcgagaag gaagcgacga tcaacgacga
     4561 gatcgaccgg atgcgcctct cggcgacccg ctcgctcttc gagcggcgcg acgtgctcat
     4621 cgtggcgagc gtctcgtgca tctacggcct gggctctccc gaggcttact acgggatgat
     4681 gctgcccctc gagatcgggc agcggatcga gcgcgagcag atcctgcgga agctcgtgga
     4741 catccagtac gagcgcaacg acgtcgagtt cgggcggggc accttccggg tgcgcggcga
     4801 catcatcgag gtctacccgt cctacgagga gcaggcggtg cgcatcggcc tgttcggcga
     4861 cgaggtggac gacctggtgg tgttcgatcc gctcaccggg cgcacctccc ggcgccacga
     4921 tcggatcgcg atctacccga agtcgcactt cgccacccca cgggagcgca cgcggcaggc
     4981 gattgcctcg atcaaggccg agctggcgga gcgccgcgcg cagctcgagt cggagggcaa
     5041 gctgctcgag gcgcagcggc tccaccagcg gacgatgttc gacctggaga tgatccgcga
     5101 gatcggctac tgccacggca tcgagaacta cgcccgccac ctgaccggcc gggccccggg
     5161 ccagccgccc cccacgctgc tcgactacct gccgcccgac gcggtcgtgt tcgtggacga
     5221 gagccatcag acggtgccgc agatccgcgg catgtaccac ggcgaccggt cgcgcaagga
     5281 ggtgctggtg gagtacggct tccgcctgcc ctccgcgctc gacaaccggc cgctcaactt
     5341 cgaggagtgg gagagccgga cgcaccaggt ggtcttcgtt tccgcgacgc cggggccgta
     5401 cgagctgcgc tgctcgggag gcgtggtcgt cgagcagatc atccggccca ccgggctgac
     5461 cgacccgccg atcgaggtga ggccggtgaa ggggcaggtg gacgacctgc tcggcgagat
     5521 ccggcggcgc gccgagtcgc gcgagcgcgt gctggtgacg acgctgacca agcggatggc
     5581 ggaagacctg acggcgtact accaggagct tggcgtgcgg gtgcgctacc tgcactcgga
     5641 catcgacacg ctcgagcggg tggagatcct gcgcgacctg cggcgcggcg agttcgacgt
     5701 cctggtgggc atcaacctgc tgcgggaggg gctcgacctg cccgaggtct cgctggtcgc
//
LOCUS       DAOPQX010000224         5714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15438_length_5714_cov_4.704718, whole genome shotgun sequence.
ACCESSION   DAOPQX010000224 DAOPQX010000000
VERSION     DAOPQX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15438_length_5714_cov_4.704718"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PKK95_11745"
     CDS             <1..272
                     /locus_tag="PKK95_11745"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOC18937.1"
                     /translation="CRGEIGERLLRPVPDVTRLLDRLEAMGLVARARGGDDRRYVTTR
                     ITKAGLGVLGELEAEIDAAHARQFAHLGDGRLKTLIGLLGQVRDR"
     gene            382..936
                     /locus_tag="PKK95_11750"
     CDS             382..936
                     /locus_tag="PKK95_11750"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOC18938.1"
                     /translation="MSTTIATTTDKTTYSIDKAHSEVSFQVRHLLTRVRGRFSDFDGT
                     IAYDEAAPEQSSVRLEIRTASIDTNEPARDKHLRSDDFFAADRFPAITFASTKMVKKG
                     EDAFEVTGNLTIRDVTKEITLPVTFLGKAKDPWGNERLAFEVEYALNRKDYGLNWNAA
                     LETGGFLVGDEVKISVAVQAVPRT"
     gene            complement(939..3098)
                     /locus_tag="PKK95_11755"
     CDS             complement(939..3098)
                     /locus_tag="PKK95_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18939.1"
                     /translation="MRTALRRPWLLPTLPLLLVPVLGGEAGRPAPSPIDRRALVSRHD
                     PVLRSMDADSPLSIGNGQFAFTADVTGLQTFAEAYEQTVPLGTLAQWGWHRAPNPGGW
                     TIDSYRFTEYESHGRKVGYADIPGDRRTPEVQWLRANPHRLHLGRIGFELRTKDGRTA
                     APADLTGIEQRLDLWSGTLESRFSLDGEPVEVITICHPAIDAVAVRVRSALVREGRLA
                     IRLAFPYGTGEIVTADWSRPEAHQTVVHLAGPRTARLTRRLDEDVYSVSAAWGPSGTL
                     VERGRHEFVVEPGGGEAFEFVSAFAPAATAGDAGPPSFDDTLAAAREHWKRFWSEGGA
                     IDLSGSRDPRWRELERRIVLSQYLTAIQCSGELPPQETGLTFNSWYGKFHLEMHWWHA
                     AHFALWNRLPMLERSLGYYQRILPRARGTAARQGYRGARWPKMTGPEGAESPSPIGPL
                     LVWQQPHPIFYAELVHRARPGRETLERFREVVFETAEFMASYAAWDEEAGRYVLGPPL
                     QCAQEIFPAAETVNCAFEVAYWRWGLQAAQRWRERLGLAREARWDEVLTKLSGLAVHD
                     GKYVFAETAPASFDDPKWARDHPSVLGALGVLPGEGVDPATMRRTFDWIWEHWSWADT
                     WGWDYPMIAMTAARLGDGRRAVDALLMDAPKNVYRPNGHNYQRPGLTIYLPGNGGLLY
                     AAAMMAAGWDGSPDRPAPGFPGDGSWTVRWEGLRRAF"
     gene            3290..5419
                     /locus_tag="PKK95_11760"
     CDS             3290..5419
                     /locus_tag="PKK95_11760"
                     /inference="COORDINATES: protein motif:HMM:NF019130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HOC18940.1"
                     /translation="MVHRSLVLAFLAVLAWGAGLPPPAAAETGYDLWLRYTPVPDAAL
                     CDSYRRSASSVVVTIDSPTGRAIADELRRGLEGMLGAPIRFSARIERDGAILVGTPAA
                     SRLIAGFGWADRLERAGHEGFVIRSAFAEGRRVTVIASRGESGALHGAFHFLRLLQTR
                     QPIEQLDIVERPRVGLRLLNHWDNLDGSIERGYAGRSLWDWARLPRVLDPRLRDYARA
                     NASIGINGTVLNNVNANPRSLTRPYLEKAAAIASVLRPYGIRIFLSANFAAPRLLGKL
                     PTADPLDPRVAAWWRAKAVEIYSLVPDFGGFLVKANSEGQPGPQDYGRTHADGANVLA
                     DALAPHGGVVMWRAFVYSEKVDPDRVKRAYMEFVPLDGRFRDNVIVQVKNGPLDFQPR
                     EPFSPLFGATPRTPLMAELQVTQEYLGHSNHLVYLGTMWEEFFRADTYASGPGSPVAR
                     VVDGTVHRYRRTGIAGVANTGSDTNWTGHHFGQANWYSYGRLAWNPDLSAAAIADEWI
                     PMTWSDAPRVRETIRSIMLGSREALVDYSMPLGLHHLIGGDHYAPMPENPDPRREDWS
                     AIYYHRADRNGIGFDRTRRGSGAVDQYRKPLSDRFDDPATCPENLLLWFHRLPWDHTL
                     RSGGTLWEGLVRHYGRGVASARSMLSAWEGLRGEVDPERFEAVLGKLRIQASDAEAWR
                     DKCLRYFQQFSGRPLPGEAPPGPRQER"
     gene            5426..>5714
                     /locus_tag="PKK95_11765"
     CDS             5426..>5714
                     /locus_tag="PKK95_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC18941.1"
                     /translation="MPDPTQKVGVREKVGYAMGDIATNFFFQSMILYQTRFYTDTVGL
                     SAVAVGTMFLVLRLADALFDPAIGALADRTRTRWGSFRPWLLWTAVPFGVLF"
BASE COUNT          838 a         2078 c         1949 g          849 t
ORIGIN      
        1 tgtgccgcgg cgagatcggc gagcggctgc tgcggcccgt tcccgacgtc acgagactgc
       61 tcgaccgcct cgaggcgatg gggctggtgg cgagggcgcg cggcggcgac gaccgccgct
      121 acgtgacgac ccggatcacg aaggccggcc ttggcgtgct cggcgagctg gaggcggaga
      181 tcgacgccgc gcacgcccgg cagttcgccc acctcggcga tggccgcctg aagaccctca
      241 tcgggctcct cgggcaggta cgcgaccgct agtgcatgcc gaacacgggc caccccgggt
      301 ggcggaacga gcggccggcg cgggctttag gtgttatcac acatgaacag gaaggacctg
      361 ggcaactgag aggagaactg catgagcacc acgatcgcga cgaccaccga caagacgacc
      421 tattccatcg acaaggctca ctcggaagtg agcttccagg tgcgccacct gctcacgaga
      481 gtgaggggcc gtttctcgga cttcgacggg acgatcgcct atgacgaggc cgcgcccgag
      541 cagtcgtcgg ttcgcctcga gatccggacg gcgagcatcg acaccaacga gcccgcccgc
      601 gacaagcacc tgcggtccga cgacttcttc gcggccgacc gcttcccggc gatcaccttc
      661 gccagcacga agatggtgaa gaagggcgag gacgccttcg aggtgaccgg aaacctgacg
      721 atccgcgacg tcaccaagga gatcacgctg cccgtgacct tcctcggaaa ggccaaggac
      781 ccctggggca acgagcggct cgccttcgag gtggagtacg cgctgaaccg caaggactac
      841 ggcctcaact ggaacgccgc cctcgagacc ggcgggttcc tggtgggtga cgaggtcaag
      901 atcagcgtgg cggtgcaggc cgttccgagg acgtaagctc aaaacgcccg gcgcagcccc
      961 tcccaccgga ccgtccacga cccgtcgccc gggaagccgg gcgccggacg gtccgggctc
     1021 ccgtcccagc cggccgccat catggccgcg gcgtagagga gcccgccgtt tccgggcagg
     1081 tagatcgtca gcccgggccg ctggtagttg tggccgttcg ggcggtacac attcttcgga
     1141 gcatccatga gcagcgcgtc cacggcgcgc cgcccgtctc cgagcctggc cgccgtcatg
     1201 gcgatcatcg ggtagtccca gccccaggtg tcggcccagc tccagtgctc ccagatccag
     1261 tcgaaggtgc ggcgcatggt cgctggatcg acgccctcgc ccggcagcac gcccagggcg
     1321 cccagaacgg acggatgatc ccgcgcccac ttcgggtcgt cgaagctggc cggcgcggtt
     1381 tcggcgaaca cgtacttgcc gtcgtgcacg gcgagtcccg agagcttcgt cagcacctcg
     1441 tcccaccgcg cctcgcgcgc cagccccagc cgctcgcgcc atcgctgcgc cgcctgcagg
     1501 ccccagcgcc aataggccac ctcgaacgcg cagttcacgg tctcggcggc ggggaagatc
     1561 tcttgcgcgc attggagcgg cgggccgagc acgtagcggc ctgcctcttc gtcccaggcc
     1621 gcgtagctcg ccatgaactc ggcggtctcg aacaccacct cgcggaagcg ctcgagcgtc
     1681 tcgcgcccgg gcctcgcccg gtgcaccagt tcggcgtaga agatcgggtg cggctgctgc
     1741 cacacgagca gcggcccgat cggtgacggc gactcggccc cctcgggacc ggtcatcttc
     1801 ggccaccgcg ccccccggta tccctggcgc gcggccgtgc cccgcgccct cggcaggatc
     1861 cgctggtaat agccaaggct ccgctccagc atcggcaggc ggttccagag cgcgaagtgg
     1921 gcggcgtgcc accagtgcat ctcgaggtgg aacttgccgt accagctgtt gaaggtcagc
     1981 ccggtctcct gcggcggcag ctcccccgag cactggatgg ccgtcaggta ctgcgacagc
     2041 acgatgcggc gttccagctc gcgccacctc gggtcgcggc tgcccgacag gtcgatcgct
     2101 ccgccctcgc tccagaaccg cttccaatgc tccctcgccg cggcgagcgt gtcgtcgaac
     2161 gagggtggcc cggcatctcc ggccgttgcg gcaggcgcga acgccgacac gaactcgaac
     2221 gcctccccac ctcccggttc gacgacgaac tcgtggcgcc cgcgctcgac cagcgtgccg
     2281 gacggtcccc acgcggccga caccgagtag acgtcttcat ccaggcgcct cgtgagccgg
     2341 gccgtccgcg gtcccgcgag atgcacgacg gtctggtgcg cctcgggccg tgaccagtcg
     2401 gccgtgacga tctcgccggt gccgtacgga aacgccagcc ggatcgcgag gcgcccttcc
     2461 cgcacgaggg ccgatcgaac gcggactgcc accgcgtcga ttgccgggtg acagatggtg
     2521 atcacctcga ccggctcgcc gtcgagcgag aaccggctct cgagggtgcc gctccagagg
     2581 tcgagcctct gctcgatgcc cgtcaggtcg gcaggcgccg cggtgcgccc atccttcgtc
     2641 cgaagctcga agccgatgcg gccgaggtga aggcgatgag ggttggcccg cagccactgc
     2701 acctcggggg ttcggcggtc gcccgggatg tcggcgtatc cgaccttccg gccgtggctc
     2761 tcgtactcgg tgaagcggta cgagtcgatt gtccagcccc ccgggttcgg cgccctgtgc
     2821 catccccact gcgcgagcgt gccgagcggc accgtctgct cgtaagcctc cgcaaaggtc
     2881 tgaagtcccg tcacgtcggc cgtgaaggcg aactgcccgt tcccgatcga caagggggag
     2941 tcggcgtcca tcgagcgcag caccgggtcg tgccgcgaca cgagcgcccg gcgatcgatt
     3001 ggcgaagggg cagggcggcc ggcctcgccg ccgagcaccg gcaccagcag cagcggcagc
     3061 gtcgggagca gccacgggcg gcggagagcg gtcctcatgg cgggccatga taccccggcg
     3121 gaccgggggg gccggcgcga cgcggcgccg cgccgtaacg gttgcacgcc ccgcgcggcc
     3181 cgaatatagt tgtcgccggc aacgattggt gcggaggggg cgagcgcgac gcggtgagcc
     3241 gcttcgtcct gattctcggc aacctcctca tttagttgtg ggcgcgaaca tggtgcatcg
     3301 ttccctggtc ctcgcgttcc tcgcggtgct cgcgtggggg gcgggcctcc cgcccccggc
     3361 tgccgccgag accggctacg atttgtggct ccgctacacg ccggttccag acgccgcgct
     3421 gtgcgactcg taccgccgct ccgcgtcctc ggtcgtggtg acgatcgatt ccccgaccgg
     3481 acgggccatc gccgacgagc tgcggcgtgg cctcgagggg atgctcggcg cgcccatccg
     3541 cttttcggcc cgcatcgagc gggacggcgc gatcctggtc gggaccccgg ccgcctctcg
     3601 cctgatcgcg ggcttcggct gggcggaccg cctcgagcgg gccggacacg aggggttcgt
     3661 gattcggtcg gcgttcgccg agggccgccg ggtcaccgtc atcgcctcgc gcggcgagtc
     3721 cggcgccctg cacggggcct ttcacttcct gcgcctcctg cagacccgcc agccgatcga
     3781 acagctcgac atcgtggagc gtccccgcgt cgggctgcgg ctgctgaacc actgggacaa
     3841 cctcgacggc agcatcgagc gcggctacgc gggccgctcg ctctgggact gggcgcggct
     3901 gccgagagtg ctcgatcccc gccttcgcga ctacgccagg gccaacgcct cgatcggcat
     3961 caacggcacc gtcctgaaca acgtcaacgc caacccgcgg tcgctcaccc gcccctacct
     4021 cgagaaggcg gcggctatcg cctcggtgct ccgcccctac ggcatccgca tcttcctctc
     4081 ggccaacttc gcggcccccc gcctgctcgg caagctgccg accgccgatc ctctcgaccc
     4141 gcgcgtggcg gcctggtggc gcgccaaggc ggtggagatc tacagcctcg tgcccgattt
     4201 cggcggcttc ctcgtgaagg cgaacagcga ggggcagccc gggccccagg attacggccg
     4261 gacgcacgcc gacggcgcca acgtgctcgc cgacgccctt gccccgcacg gcggtgtcgt
     4321 gatgtggcgg gccttcgtgt actccgagaa ggtggacccc gaccgggtga agcgcgccta
     4381 catggagttc gtcccgctcg acggccggtt tcgcgacaac gtcatcgtgc aggtgaagaa
     4441 cggccccctc gacttccagc cgcgcgagcc tttcagcccg ctgttcggcg cgacgccccg
     4501 gacgccgctg atggccgagc tgcaggtgac gcaggagtac ctcggtcact cgaatcacct
     4561 cgtgtacctc gggacgatgt gggaggagtt ctttcgggct gacacgtacg cctctggccc
     4621 gggctcgccg gtcgcgcgcg tggtggacgg cacggtgcac cgctaccgcc gcaccggcat
     4681 cgcgggcgtc gccaacacgg gcagcgacac caactggacg ggccaccact tcggccaggc
     4741 caactggtac tcgtacggcc gtctcgcctg gaaccccgac ctctcggccg ccgccatcgc
     4801 cgacgagtgg atcccgatga cctggtcgga cgcccctcgc gtgcgcgaga cgatccgctc
     4861 gatcatgctc gggtcgcgcg aggcgctcgt ggactactcg atgccgctcg gcctccacca
     4921 cctgatcggc ggcgatcact acgccccgat gcccgagaac cccgatcccc gccgcgagga
     4981 ctggtcggcg atctactacc accgggccga ccgcaacggc atcggcttcg accggacgcg
     5041 ccgggggagt ggggcggtgg accagtaccg caagccgctg agcgaccggt tcgacgaccc
     5101 ggccacctgc cccgagaacc tcctgctctg gttccaccgc ctgccctggg accacacgct
     5161 ccgctccggg ggcacgttgt gggaggggct cgtgcgccac tacggccggg gcgtggcgtc
     5221 ggcgcggtcg atgctctcgg cctgggaagg cctgcggggc gaggtcgatc ccgagcgctt
     5281 cgaggcggtg ctcggcaagc tgcgcatcca ggcttcggat gccgaggcct ggcgcgacaa
     5341 gtgcctccgg tacttccagc agttcagcgg gcgcccgctg ccgggagagg cacctcccgg
     5401 cccgcgccag gagagatgag gacccatgcc tgaccccacg cagaaggtcg gtgtgaggga
     5461 gaaggtcggc tacgcgatgg gggacatcgc gacgaacttc ttcttccagt cgatgatcct
     5521 ctaccagacc cggttttaca ccgacacggt cgggctgtcg gccgtggccg tcgggacgat
     5581 gttcctggtt ctccggctcg ccgacgcgct gttcgacccc gcgatcggcg ctctcgcgga
     5641 ccggacgcgc acgcggtggg gcagcttccg cccctggctg ctgtggacgg cggttccctt
     5701 cggcgtgctc ttct
//
LOCUS       DAOPQX010000225         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15471_length_5705_cov_4.799115, whole genome shotgun sequence.
ACCESSION   DAOPQX010000225 DAOPQX010000000
VERSION     DAOPQX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15471_length_5705_cov_4.799115"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..374
                     /locus_tag="PKK95_11770"
     CDS             111..374
                     /locus_tag="PKK95_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18942.1"
                     /translation="MLFQPTVPPNTPLTPIIVQQVDAPTEEIGVVDILVGSLGITGLL
                     LLASALLGIATAAVLFWLRRRYGEEPDAASLASAQLNLTPHKK"
     gene            complement(424..1962)
                     /locus_tag="PKK95_11775"
     CDS             complement(424..1962)
                     /locus_tag="PKK95_11775"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOC18943.1"
                     /translation="MPSPAIDPSFPISFLRLSETHAGWAAVPLFEDEDPGTLAGDPAL
                     AAGVARAVAATGFVARRFEALVVPADEKEFKADALVLLGAGKVDEFTPDVARRLASTC
                     ALLARGRGASSVALLHRSGTGPTASLPASIWAEAWAEGATLSEFDPGRYKTAEEERKA
                     MPPVTLVWPGLPEAERGGARSAASRGQLVVRCANLARDLVNEPGNLLPPRLLAERARS
                     LADGTSLRVEVLEEPALHELGMGLLLGVARGSHEPPRLIVVEHAPAGAPASPVLGFVG
                     KGVTFDAGGISIKTAGGMDRMKDDMAGGAAVLAALRAISLIDIPLRVIGVVPSVENLP
                     SGRALKPGDVLRGASGRTVEVLDTDAEGRLILGDALWYARQRGATHLVDVATLTGSCG
                     IALGKTTTGLFGRPDDWADRVRALAEERGERSWRLPLVDEYKELLKSEIADTVNIGGR
                     YGGSITAALFLMEFAGEDLPWVHLDIAATAWNDEARPYLPKGPTGVGVRTLTALAAAM
                     ARQA"
     gene            2035..2472
                     /locus_tag="PKK95_11780"
     CDS             2035..2472
                     /locus_tag="PKK95_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18944.1"
                     /translation="MKSNTFARWARLGLVLGIGVFLGAGAQAQGVGERSKGKKPELIV
                     RASPMVGFAPAKVQFLAILQGGDDDYEEYYCTGIEWDWDDDTVSQSTPDCDPYEPGES
                     RITRRYSATHVFRLDGRYEVKFKLKRKNDVLALASVTIQVQPG"
     gene            complement(2476..3894)
                     /locus_tag="PKK95_11785"
     CDS             complement(2476..3894)
                     /locus_tag="PKK95_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOC18945.1"
                     /translation="MSAASSRALVLASLLGLLVSSATTATAAVNLLPAEPVQESGSDR
                     LRDAIHGRFTVLEVTGGVVLVPRTPRAGVDSIELRDRTVAINGSEVTGAELRERLGRD
                     ADAVIELTYLEPRERRQLLLEERRAPQSGPEAPSVLPPVPETPPKRLPARRTSPERTY
                     ERRTEGRVRIGGDIRVEDDEQVNGPVVAVLGSVYVNGRVSDAVVAVGGGVTLGPRAEI
                     RGDVTSVGGAVSRDPSAAVSGQINSVQFQVPHVSVRPIRWPFSVPAVWDREPWHGFRL
                     MGTLLRIALFALLAVLMTLLLPGSVGRIRDSVRFEPWKCALAGLFAQLLFVPLLVIGT
                     LVLVISIVGIPLLVLVPFAVLAFFLALLLGFAGSASAFASAVVDRSSRSVPSAVGTVV
                     AGLALIWGLTVIGRVVSLGGGPLAWVAALFLVAGFLVEYVAWTMGLGGALLTRFGRHG
                     RGWGPVTPPPIPPPPVAASPEN"
     gene            complement(3894..4421)
                     /locus_tag="PKK95_11790"
     CDS             complement(3894..4421)
                     /locus_tag="PKK95_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18946.1"
                     /translation="MSSDSHRPGAELPGWIEAEMAGRLDEADAAFARIFAASTERLAP
                     PARFADLVVERALRAREPSAAWLGVPVRLLIGLAMVLMGLPIAFISGAGLFELLAGPG
                     PRLASAFARFVGLVGGAASAAGAAWSVASAVGEAVLQACATGPVPLILLLNVLLAVAG
                     YVGLRRLLAPAKECW"
     gene            complement(4427..5080)
                     /locus_tag="PKK95_11795"
     CDS             complement(4427..5080)
                     /locus_tag="PKK95_11795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC18947.1"
                     /translation="MCLPGARGFPKAGALTDAELVERALAGAEDGYRGLVTRYQRPVY
                     NLLVQMLRNPALAEELAQDTFLRAFSRLGTYDARYRFINWILRIAHNLAVDALRRRSV
                     SELSADDPNSPELAARVTNAPDPDADAGSEAERRQLARALDAALARLRPEYRRLVILR
                     YHEDLSHDDISAVTGLPVGTVKSYLHRARAEMARHLEAAGWGRNPAVQPPGPGGRRS"
     gene            complement(5150..>5705)
                     /locus_tag="PKK95_11800"
     CDS             complement(5150..>5705)
                     /locus_tag="PKK95_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOC18948.1"
                     /translation="ANHVPPGVHITLHSENGMLGVGPYPLESQVDPDLVNAGKETISE
                     TPGCCYFSSADSFAMIRGGHIDLTVLGALEVDQEGNLANWMIPGKMVKGMGGAMDLVV
                     GARRVIVAMEHTTRDGGHKILERCRLPLTGARVVDSIVTELAVIKVTPDGLLLQEIAE
                     GTSIEQVRAATGAPLRIEGTPQRF"
BASE COUNT          886 a         2084 c         2005 g          730 t
ORIGIN      
        1 gctaccgcca gagggtggcc gaggcgctct tcgagggaat ccgcgggtac cagaagtcgc
       61 tcaagcgcgc gctggaagtc tcgcgcgaag actagatggt acaattggcc gtgttgttcc
      121 agccgaccgt cccaccgaac acgccgctga cgccgatcat cgtgcagcag gttgacgcgc
      181 cgaccgagga gatcggtgtc gtcgacatcc tggtgggttc gctcggcatc acgggcttgc
      241 tgctgctggc ctcggcgcta ctgggaatcg ctacagcggc tgtcctcttc tggctgcgcc
      301 ggcgatacgg cgaggaaccc gacgccgcct cgctcgcctc ggcccagctc aacctcaccc
      361 cgcacaagaa gtagcgcagg ccctacgctt gcgatccgtt cacggcccgg cgccgcggcc
      421 gcctcacgcc tggcgcgcca tcgcggccgc cagcgcggtg agggtgcgca cacccacgcc
      481 ggtcggcccc ttcggcaggt aggggcgggc ctcgtcgttc cacgcggtcg ccgcgatgtc
      541 gaggtggacc cacggcaggt cctcgccggc gaactccatc aggaacaacg ctgccgtgat
      601 ggaaccgccg taccgtcccc cgatgttgac cgtgtcggcg atctcgctct tcagcaactc
      661 cttgtactcg tccaccagcg gcagccgcca gctgcgctcg ccgcgctcct cggcgagggc
      721 gcgaacgcga tcggcccagt cgtcggggcg tccgaacagc cccgtcgtcg tcttgccgag
      781 ggcgatgccg cacgaaccgg tcagcgtggc gacgtcgacc aggtgcgtgg ccccccgctg
      841 acgcgcgtac cacagcgcat caccgaggat gagccggccc tctgcgtccg tgtcgagcac
      901 ctccaccgtg cgcccgctgg cgccgcggag cacatcgccc ggcttcagcg cccgcccgga
      961 cggcaggttc tccaccgacg gcaccacgcc aatcacgcga agcgggatgt cgatcagcga
     1021 gatcgcccgc aacgcggcca gcacggcggc gccgccggcc atgtcgtctt tcatccggtc
     1081 catgccgccg gcggtcttga tggagatgcc gccggcatcg aaggtgaccc ccttcccgac
     1141 gaaccccagg accggcgacg ccggcgcgcc ggccggcgcg tgctcgacca cgatcagtcg
     1201 cggcggctcg tggctgccgc gcgcgacgcc gagcaggagc cccatgccca gctcgtggag
     1261 agctggctcc tcgagcacct cgacgcgaag cgaggtcccg tcggccagcg agcgcgcgcg
     1321 ctcggcaagc aggcgcggcg gcagcaggtt gccgggttcg ttcaccaggt cgcgcgccag
     1381 gttcgcgcac cgcaccacca gttggccgcg cgaggcagcc gagcgggcgc ccccgcgttc
     1441 ggcctctggc agccccggcc acaccagcgt gaccgggggc atcgccttgc gctcctcctc
     1501 cgccgtcttg taccggcccg ggtcgaactc ggacagcgtc gccccttcgg cccacgcctc
     1561 cgcccagatc gaggccggga ggctggccgt gggccccgta ccggaccggt gcagcagggc
     1621 gacgctcgag gcccccctgc cccgggccag gagcgcgcag gtcgacgcca ggcgccgcgc
     1681 aacgtcgggg gtgaactcat ccaccttgcc cgcgccgagc agcacgaggg catccgcctt
     1741 gaactccttc tcgtcggccg gcactacgag cgcctcgaag cggcgggcca cgaagccggt
     1801 cgccgccacc gcgcgggcca cgccggcggc gagcgccggg tcgccggcga gcgtgcccgg
     1861 gtcctcgtcc tcgaacaacg gcacggccgc ccagcccgcg tgtgtctcgc tcagccgcag
     1921 gaaggagatg ggaaaggacg ggtcgatcgc aggcgagggc atcggagaat tgtagtgccg
     1981 cttgttggag ggtcacaagc ggcggtacaa tcacttcaac tcaccgcgag gaccatgaaa
     2041 tcgaacacat tcgcgcgctg ggccaggctg ggcctggtgc tgggcatcgg cgtcttcctc
     2101 ggtgcgggcg cccaggccca gggcgtcgga gaacgatcga agggcaagaa gcccgagctg
     2161 atcgtgcgcg cctcgcccat ggtcgggttc gccccggcca aggtccagtt cctcgcgatc
     2221 ctgcagggcg gggacgacga ctacgaggag tactactgca ccggcatcga gtgggactgg
     2281 gacgacgaca ctgtctcgca gtccacgccc gactgcgatc cctacgagcc cggtgagagc
     2341 cgcatcaccc ggcgctactc ggccacccac gtctttcggc tggacgggcg ctacgaggtg
     2401 aagttcaagc tgaagcgcaa gaacgacgtg ctcgccctgg ccagcgtgac gatacaggta
     2461 cagccgggat agaggttagt tctcgggcga cgccgcaacc ggcggcggcg ggatcggcgg
     2521 cggtgtgacc ggtccccacc cgcggccgtg gcgcccgaat cgcgtcagca gcgccccgcc
     2581 gaggcccatc gtccacgcca cgtactccac caggaagccc gcgacgagga acagcgcggc
     2641 cacccacgcc aacgggcccc cgccgagcga caccacgcgg ccgatcaccg tcaggcccca
     2701 gatcagcgcg agccccgcaa ccaccgtccc cacggcgctc ggcacgctgc gtgacgagcg
     2761 gtccaccacc gcgctggcga aggccgacgc cgacccggcg aagccgagca gcagtgccag
     2821 gaagaacgcg agcaccgcga aggggacgag caccagcagc gggatcccga cgatggagat
     2881 caccagcacg agggtgccga tcaccagcag gggcacgaac aacagctgtg cgaagagccc
     2941 cgcgagcgcg cacttccacg gctcgaaccg cacggagtcg cggatgcgcc ccaccgagcc
     3001 gggcagcagc agcgtcatca ggacggcgag cagggcgaac agcgcgatcc ggaggagcgt
     3061 gcccatcagg cggaagccgt gccacggctc ccgatcccag acggccggga ccgagaacgg
     3121 ccagcggatc gggcggacgc tgacgtgagg cacctggaac tgcaccgagt tgatctggcc
     3181 cgagacggcg gcgctcggat cgcgcgacac ggcgccgccg accgacgtca cgtcgccgcg
     3241 gatctcggcg cggggcccca gggtgacgcc cccgccgacc gcgaccaccg cgtcgctcac
     3301 gcggccgttg acgtagaccg aaccgaggac cgccacgacc ggcccgttca cctgttcgtc
     3361 gtcctcgacg cggatgtcgc cgccgatgcg cacccgcccc tcggtgcggc gctcgtacgt
     3421 ccgttcggga gaggtccggc gcgctggcag ccgcttgggc ggcgtctcgg gaacaggcgg
     3481 aagcaccgac ggcgcttctg ggccgctttg aggggcgcgc cgctcctcga ggaggagctg
     3541 ccgccgctcg cgcggctcca ggtaagtcaa ctcgatgacc gcgtcggcgt cacggccgag
     3601 ccgctcgcgc aactcggcac cggtgacctc ggacccgttg atggcgacgg ttctgtcccg
     3661 cagctcgata ctgtcgacgc ctgcccgcgg cgttcgcggc acgagcacga cgcccccggt
     3721 cacctccagc acggtgaagc gcccgtgaat cgcgtcccgc agccggtcgc ttccactctc
     3781 ctggacgggt tcggccggca ggaggttcac ggcggcggtc gcggtggtcg ccgatgacac
     3841 caggagtccg agcaggctcg cgaggacgag ggctcgagaa gatgcggcgg acattaccag
     3901 cactcctttg cgggagcgag cagacggcgc aagcccacgt acccggcgac ggccagcaga
     3961 acgttgagca gcaggatgag gggcaccggg ccggtggcgc acgcctggag caccgcctcc
     4021 ccgaccgcgg aggcgaccga ccaggcggcg ccggcggcgg aggctgcccc ccccacgagg
     4081 cccacgaagc gggcgaaggc cgaggcgagc cgtggcccgg ggcccgcgag cagttcgaac
     4141 aggccggcgc ccgagatgaa cgcgatcggc agacccatca acaccatcgc caggccgatc
     4201 aggaggcgca ccggcacacc gagccacgcc gccgagggtt cgcgcgcgcg caaggcgcgt
     4261 tcgacgacca ggtcggcgaa gcgggccggc ggggcgagcc gctcggtgct ggcggcgaag
     4321 atccgggcaa aggccgcgtc ggcctcgtcg aggcggccgg ccatctcggc ctcgatccag
     4381 ccgggcagtt cggctcccgg gcgatggctg tcgctgctca tcactgtcaa ctcctacggc
     4441 cccccggtcc gggcggttgc acggccgggt tccggcccca gccggcggcc tccaggtggc
     4501 gcgccatttc ggcccgcgcc cggtgcaggt aactcttcac cgtgccgacc ggcaggccgg
     4561 taacggccga gatgtcgtcg tggctgaggt cctcgtggta ccggaggatc accaggcggc
     4621 ggtactcggg ccgcaggcgg gcgagcgcgg catcgagggc gcgcgccagc tgacgccgct
     4681 ccgcctcgct gccggcgtcg gcgtccgggt cgggggcatt ggtgacgcgg gccgccagct
     4741 cgggcgagtt cgggtcgtcg gccgacagct cggaaaccga gcggcgccgc aacgcgtcca
     4801 ccgccaggtt gtgggcgatg cgcaggatcc agttgatgaa ccggtagcgg gcatcgtagg
     4861 tgcccaggcg cgagaacgcc cggaggaagg tgtcttgcgc cagttcctcg gccagcgcgg
     4921 ggttgcgcag catctgcacg aggaggttgt agacgggacg ctggtagcgc gtcaccagtc
     4981 cgcggtagcc gtcttcggcg ccggccaggg cccgctcaac cagctccgcg tcggtcaggg
     5041 cacccgcctt tgggaagccg cgcgcaccgg gcaggcacac cgcctcccgc gtgacgcggc
     5101 cgtcaagacg acaagacgcc ggctcgggcg cgaaagttcc acgcgccggt cagaaccgct
     5161 gcggcgtccc ctcgatccgc aggggggcgc cggttgccgc ccgcacctgc tcgatcgacg
     5221 tcccctcggc gatctcctgc agtaacaggc cgtccggggt gaccttgatg accgccagct
     5281 cggtcacgat ggaatcgacg acccgcgcgc cggtgagcgg aaggcggcag cgctcgagga
     5341 tcttgtggcc cccgtcgcgg gtggtgtgct ccatcgccac gatcacgcgc cgggcgccga
     5401 ccaccaggtc catcgcccct cccatgccct tgaccatctt gccggggatc atccagttgg
     5461 cgaggttccc ctcctggtca acttcgagcg cccccagcac cgtgaggtcg atgtgcccgc
     5521 cgcggatcat ggcgaacgag tcggcgctgc tgaagtaaca gcacccgggc gtctcggaga
     5581 tcgtttcctt ccccgcgttg accaggtcgg ggtccacctg cgactcgagg gggtagggcc
     5641 ccacgccgag catgccgttc tcggagtgca gggtgatgtg cacgccgggc gggacgtggt
     5701 tggcg
//
LOCUS       DAOPQX010000226         5705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15472_length_5705_cov_4.280354, whole genome shotgun sequence.
ACCESSION   DAOPQX010000226 DAOPQX010000000
VERSION     DAOPQX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15472_length_5705_cov_4.280354"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKK95_11805"
     CDS             <1..546
                     /locus_tag="PKK95_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18949.1"
                     /translation="ALADAEMVLPTALTTLFPWWLAGILLAGAVAAMVSTAESMLIVA
                     GTSISQDVYKGLIKKGQAPDARLLAISRASTFAVGVLGLGLALTTERLIYFIVSYAWA
                     GIGCSFAPAVLLSFYWDRFTSLGVVTALVTGLVTTIVWIATGFDQRLTAMAVTFFVSM
                     GAAVLVTLAVPRNSRQQTVRS"
     gene            631..1614
                     /locus_tag="PKK95_11810"
     CDS             631..1614
                     /locus_tag="PKK95_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HOC18950.1"
                     /translation="MTMKTLALAAVIGLAAGTASADVHQFTPKAGVQTFAVRPPVLKV
                     RPGDTIVSPTFSLPGDYYERDGGAWPGEVGPFHVEGAAPGDTLVVRILKVELNRDTAV
                     SAIRPGGLSALATDGRLRLLNDPLPSRRFVWQLDRKRNVGILGLPGSASKRIEIPLAP
                     MVGRVAVAPAGEEAFGGLWPGAFGGNMDVPDVREGATVFLPVFHEGALFYFGDIHAAQ
                     GDGEVCGSGLETTGVVTMQFELVKGKRIAWPRIEDATHIMVAGSARPLIDAFRIAHVE
                     LIDWLAAEYGFDRIEAYQVVSQAGTARIGNVVDPLYTVAAKFPKALLPQRK"
     gene            1741..2616
                     /locus_tag="PKK95_11815"
     CDS             1741..2616
                     /locus_tag="PKK95_11815"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC18951.1"
                     /translation="MAASRLARVLVTMLCAAVAVPAMPAAAQQGQQPQQGQKQYEPEV
                     GQAGKDVVWVPTSDALVEKMLDLAEVTAKDYLIDLGSGDGRTVIAAAKRGARAHGIEY
                     NPDMVALSKKNAEAAGVSDRATFEKADLFEADFSKATVITMFLLPSINMKLRPTLLEL
                     KPGTRIVSNTFTMEDWEADETATASEGCTSWCNALLWIVPARVQGTWQTPQGRLRITQ
                     TFQTVSGSLNSSGTTTAITGKLRGDQITFTAGKRQYTARVNGNTLEGTVTAADGQAQP
                     WKATKVVATSGTPRP"
     gene            2621..3889
                     /locus_tag="PKK95_11820"
     CDS             2621..3889
                     /locus_tag="PKK95_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC18952.1"
                     /translation="MPSDPRTLEALRIDREAKQPGSRPGLLWGALAALLLLAIAAGWY
                     FWGAPGPVEVRAVTVEQPSQGPGAGTVLNASGYVTARRRATVSSKVTGRVVQVHVEEG
                     MDVRRGQVLARLDPTTAERALALAEAELGAARRAVAEYEVRLDQARLNAGRVQQLSAE
                     GIATKAQHDDADAEVKALQARLALAAEQVRVSERQVALRQADLSDTVIVAPFSGVAIS
                     KDAQPGEMISPVSAGGGFTRTGICTLVDMESLEIEVDVNESYIHRVAAGQRVEAVLDA
                     YPDWRIPAHVITMVPTADRQKATVLVRIGFDRAAAAGTSGPAEREAPLDPRILPDMGV
                     KVAFLGASPPPGASPSAPSPRVPAAAVRTDGGSTVVFVVKDGRAERRAVRTGSTAGEM
                     VEIMSGLTPGERIVIESPAPLKDGLAVVVR"
     gene            3955..4350
                     /locus_tag="PKK95_11825"
     CDS             3955..4350
                     /locus_tag="PKK95_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOC18953.1"
                     /translation="MTEQVIGAAIEVHRALGPGLLESAYEECLSYELTLRDVPFRRQI
                     PLPLVYKGAKLDCGYRLDLLVADALVVEIKTVEHLDPIHIAQVLTYLKMGGWPLGLIF
                     NFNLAALKNGIKRVINGSLEAAAQGLPKA"
     gene            4449..5138
                     /locus_tag="PKK95_11830"
     CDS             4449..5138
                     /locus_tag="PKK95_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC18954.1"
                     /translation="MVEPLVRTVGLHKEFRRGSERIDVLQGVDLEVQAGEFLALMGPS
                     GSGKTTLLNLLGGLDRPTRGEITVGGERIDTMSSGALARWRAAHVGFVFQLYNLLPML
                     TAERNVELPLLLTRLSRAERRRHVEAALRVVGLSDRARHYPRQLSGGQEQRVGIARAV
                     VIDPTLLLCDEPTGDLDRKSGEEVLDLLQALNRANGKTIVMVTHDPHAAARAGRVLHL
                     EKGVLTTEPAV"
     gene            5135..>5705
                     /locus_tag="PKK95_11835"
     CDS             5135..>5705
                     /locus_tag="PKK95_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18955.1"
                     /translation="MRLLPLLWSNLRRRRIRTLFTLLSIAVAFILFGYLLAIEAAFSM
                     GVELLGNERLVVIHRVSLIQLLPISYQERMRQVDGVVDVCHQTWFGGIYQDPKNFFMQ
                     IPVDAECHLRMFPEFKLPADQRAAWLADRQGAIVGRATADRFGWKVGDRIPLQATIWR
                     KKDDSSNWEFNLRGIYDADTPGTDTSQFFF"
BASE COUNT          867 a         1940 c         2008 g          890 t
ORIGIN      
        1 gcgctggccg acgccgagat ggttcttccg accgcgttga ccaccctctt cccctggtgg
       61 ttggcgggga tcctgctcgc gggcgcggtg gcggccatgg tgtcaaccgc cgagagcatg
      121 ctgatcgtgg cggggacctc gatcagccag gacgtctaca aggggctcat caagaagggc
      181 caggcccccg acgcccgcct gctcgccatc tcgagggctt cgaccttcgc ggtcggcgtg
      241 ctcggcctcg gcctcgccct caccaccgaa aggctgatct acttcatcgt ctcgtacgcg
      301 tgggccggca tcggctgctc gttcgcgccg gccgtgctgc tgtcgttcta ctgggatcgc
      361 ttcacgtcac tcggcgtggt cacggcgctc gtcacgggcc tggtcaccac gatcgtctgg
      421 atcgcaaccg gcttcgacca gcgcctgacc gcgatggcgg tgaccttctt cgtgtcgatg
      481 ggagcggccg tgctggtgac gttggccgtg ccaaggaaca gcaggcagca gacagttcgc
      541 agctgacagc gggtcacgct gcggctccgc gttgaacgtg atgatggacg tggtgcggtt
      601 tcttgatggg ggtatcacgg cgtgttgagc atgaccatga agacacttgc gctggcggcc
      661 gtgatcggcc tggccgcagg caccgcgtcg gcggacgtcc accagttcac gcccaaggcg
      721 ggcgtgcaga ccttcgccgt ccgcccgccg gtgctgaagg tcaggcccgg cgacaccatc
      781 gtctccccca ccttctcgct ccctggcgac tactacgaga gagacggcgg ggcgtggccg
      841 ggcgaggtcg gcccctttca cgtcgagggg gccgcgcccg gcgacacgct ggtggtgcgg
      901 atcctgaagg tcgagctgaa ccgcgacacg gcggtttcgg cgattcgccc gggcggcctg
      961 agcgcgctcg ccaccgacgg gcgcctgcgc ctgctgaacg accccctgcc gtcgcgccgc
     1021 ttcgtctggc agctcgatcg gaagcggaac gtgggaatcc tcggccttcc cgggtcggcg
     1081 tcgaagcgca tcgagatccc gctggccccg atggtggggc gggtcgcggt cgcgccggcc
     1141 ggcgaggagg ccttcggcgg gctgtggccg ggagcgttcg gcggcaacat ggacgtgccc
     1201 gacgtccgcg agggggcgac cgtcttcctc ccggtcttcc acgagggcgc gctcttctac
     1261 ttcggcgaca tccacgccgc ccagggcgac ggcgaggtct gcggttcggg cctcgagacg
     1321 acgggcgtgg tgacgatgca gttcgagctg gtcaagggca agcggatcgc ctggccgcgc
     1381 atcgaggatg cgacccacat catggttgcg ggcagcgcgc gcccgctcat cgacgccttc
     1441 cgcatcgcgc acgtcgagct gatcgactgg ctcgcggccg agtacggctt cgaccgcatc
     1501 gaggcctacc aggtggtctc ccaggcgggc accgcccgga tcggcaacgt cgtcgatccg
     1561 ctgtacacgg tggcggcgaa gttccccaag gcgctgttgc cgcagaggaa gtgagaggag
     1621 cggggcccgg ggggccttgc cgaagcgagg caacttgacg gcccgaggcg gcgaatgctc
     1681 tgcttgatcg gggttcagcc ctttgatccg ggcccggttc acctcgacag gaggcttacg
     1741 atggcggcgt cccgtcttgc gcgcgtgttg gtgacgatgc tctgcgcggc agtcgccgtg
     1801 ccggcgatgc ccgcggcggc ccagcagggg cagcagcccc agcaggggca gaaacagtac
     1861 gagcccgaag tcgggcaggc gggcaaggac gtggtgtggg tgccaacctc cgacgccctc
     1921 gtcgagaaga tgctcgacct tgccgaggtg acggcgaagg attacctgat cgacctcggg
     1981 tcgggtgacg ggcgcacggt gatcgcggcg gccaagcggg gcgcgcgggc gcacgggatc
     2041 gagtacaacc ccgacatggt tgcactctcg aagaagaacg ccgaggcggc gggcgtgagc
     2101 gaccgggcca ccttcgagaa ggccgacctc ttcgaggccg acttctccaa ggccaccgtc
     2161 atcaccatgt tcctgctccc ctccatcaac atgaagctcc ggccgacgct gctcgagctg
     2221 aagcccggca cccgcatcgt ctccaacacc ttcacgatgg aggactggga ggcggacgag
     2281 acggcgacgg caagcgaggg ctgcaccagc tggtgcaacg cgctgctgtg gatcgtcccc
     2341 gcccgcgtcc agggcacctg gcagacgccg cagggccggc tgcgaatcac ccagacgttc
     2401 cagacggtga gcgggtcgct caacagctcc ggcaccacca ccgcgatcac cggaaagctt
     2461 cgcggcgacc aaatcacttt caccgccggg aagcggcagt acacggcgcg ggtcaacggg
     2521 aacaccctgg aggggaccgt gaccgccgcc gacggccagg cgcagccgtg gaaggccacc
     2581 aaggtggtcg cgacgagcgg cactccgagg ccttgactcc atgccgagcg atccccgcac
     2641 cctggaggcc ctgcggatcg accgggaggc gaaacagcct ggcagccgcc ccggcctgct
     2701 ctggggggcg ctagccgccc tcctcctcct ggccattgcc gccggctggt acttctgggg
     2761 cgcaccgggc cctgtggaag tgcgggccgt gacggtcgag caaccatccc aggggcccgg
     2821 agcgggcacg gtcctgaacg cttcgggcta cgtgacggcc cggcgccggg ccacggtctc
     2881 gtcgaaggtc accgggcgcg tcgtccaggt gcacgtcgag gaaggcatgg acgtgcgccg
     2941 agggcaggtg ctcgcgcgcc tcgacccaac gaccgccgag cgcgcgctcg ccctcgccga
     3001 ggccgagctc ggggcggccc gcagagccgt cgccgagtac gaggtccggc tcgaccaggc
     3061 acggctcaat gccgggcgcg tgcagcagct ctctgccgaa gggatcgcca cgaaggccca
     3121 gcacgacgat gcggacgccg aggtcaaggc gctccaggcg cgcctggcgc tggccgccga
     3181 gcaggttcgc gtatccgagc gccaggtcgc cctccgccag gccgatctct ccgacacggt
     3241 catcgtggcg cccttctcgg gggtcgcgat ctcgaaggac gcgcagccgg gcgagatgat
     3301 ctcccccgtg tcggccggag gcggcttcac ccgcaccggc atctgcacgc tcgtggacat
     3361 ggagtcgctc gaaatcgagg tcgacgtcaa cgagagctac atccaccggg tggcagccgg
     3421 ccagcgcgtc gaggcggtgc tcgacgctta ccccgactgg cgcatcccgg cccacgtgat
     3481 cacgatggtc ccgaccgcgg accgccagaa ggcgacggtg ctggtgcgca tcggcttcga
     3541 ccgcgcggcg gccgccggga cgtccgggcc ggcagagcgg gaggcaccgc tcgacccgcg
     3601 gatcctgccg gacatggggg tgaaggtggc gttcctgggc gcctcgccgc cgccgggagc
     3661 gtcgccctct gcgccctcgc cgcgcgtccc ggctgccgcg gtgcgcaccg acggcggctc
     3721 gacggtggtc ttcgtggtca aggacgggcg cgccgagcgc cgcgccgtcc gcaccggctc
     3781 cacggcgggc gagatggtcg aaatcatgtc cggtctcacc cccggcgagc gcatcgtcat
     3841 cgaatccccg gcgcccctga aggacggcct cgccgtcgtc gtccggtaac ggtgtcgagt
     3901 tcaccacaga ggcacagaga cacagagacg acggacgcca tgggattgaa tcagatgacc
     3961 gagcaggtca ttggcgccgc aatcgaggtt caccgcgcgt tgggcccggg gcttttggag
     4021 tccgcctacg aggaatgcct ctcctacgaa ctgacccttc gtgacgttcc gtttcgtcgg
     4081 cagatccctc tgccattggt gtacaaaggt gcgaagctgg actgcggtta caggctagac
     4141 ctgctggttg cggacgcgct ggtcgtcgag atcaagaccg tcgagcatct ggatcccatt
     4201 cacattgcgc aggtgctcac gtacctgaag atggggggct ggcctctggg gctgatattc
     4261 aatttcaacc tcgccgccct caagaacggg atcaagcggg tcataaacgg atcactggag
     4321 gccgctgcgc agggccttcc gaaggcctga tggttccttg tcttgtctct gtgcctctgt
     4381 gcctctgtgg tgaacgtgat ctcaaatgca gcgggacagc gtgaccgtga tgtgaaggga
     4441 ggctgcgcgt ggtggagccg ctggtccgga ccgttggcct gcacaaggag ttccgccgcg
     4501 ggagcgagcg gatcgacgtg ctgcaggggg tcgatctcga ggtgcaggcg ggcgagttcc
     4561 tcgcgctgat ggggccctcg gggtcgggaa agacgactct cctcaacctg ctcggcggcc
     4621 tcgaccggcc cacgcggggc gagatcaccg tcggcggcga gcggatcgac acgatgtcgt
     4681 ctggggcgct cgcccgctgg cgggcggcgc acgtcggctt cgtcttccag ttgtacaacc
     4741 tcctgccgat gctcaccgcc gagcggaacg tcgagctgcc gctgctgctc acccggctct
     4801 cgcgggccga gcggcggcgg cacgtggagg cggcgctgcg cgtggtcggc ctgtccgacc
     4861 gggcccgcca ctatccccgc cagttgtccg gcggccagga acagcgcgtc ggcatcgccc
     4921 gcgcggtcgt catcgacccg acgctgctgc tgtgcgacga gccgaccggc gacctcgacc
     4981 gcaagtcggg cgaggaggtg ctcgacctgc tgcaggcgct gaaccgcgcg aacggcaaga
     5041 cgatcgtcat ggtcacccac gatccccacg ccgccgcccg cgcgggccgc gtgctccacc
     5101 tggaaaaagg cgtgctgacc acggagccgg ctgtatgagg ctgctgccgc tgctctggag
     5161 caatttgagg cggcgcagga tccggacgct gttcacgctc ctgtcgatcg ccgtcgcgtt
     5221 cattctcttc ggctacctgc tcgccatcga ggcggccttc tccatggggg tcgagctgct
     5281 gggcaacgag cggctggtcg tcatccaccg cgtgtcgctg atccagctgc ttccgatcag
     5341 ctaccaggag cggatgcggc aggtggacgg cgtggtggac gtctgccacc agacctggtt
     5401 cggcggcatc taccaggacc cgaagaactt cttcatgcag atcccggtgg acgcggagtg
     5461 ccacctccgg atgttccccg agttcaagct gcccgccgac cagcgtgccg cctggctcgc
     5521 cgaccggcag ggggccatcg tgggccgcgc gaccgccgac cggttcgggt ggaaggtggg
     5581 cgaccgcatc ccgctgcagg cgacgatctg gcggaagaag gacgactcgt cgaactggga
     5641 gttcaacctg cggggcatct acgacgccga cacgcccggc accgacacct cgcagttctt
     5701 cttcc
//
LOCUS       DAOPQX010000227         5703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15477_length_5703_cov_4.385977, whole genome shotgun sequence.
ACCESSION   DAOPQX010000227 DAOPQX010000000
VERSION     DAOPQX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15477_length_5703_cov_4.385977"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKK95_11840"
     CDS             complement(<1..180)
                     /locus_tag="PKK95_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOC18956.1"
                     /translation="MPKVQPIETNGGSGRGRSRRVASSLSEINVVPLVDVMLVLLIIF
                     MVTAPMMQRGLDVNLP"
     gene            complement(185..940)
                     /locus_tag="PKK95_11845"
     CDS             complement(185..940)
                     /locus_tag="PKK95_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOC18957.1"
                     /translation="MRTLGDLVLALQVQGPAAPEPGLGGNVIELILRSSLAAKVVLLI
                     LLVFSVVSWSIILYKLWVFRRAERHSSTFLDVFRKSSKFSEVQAVCKTLGDSPLVGIF
                     QAGYAELNAQLRQPAGAAVTGATAVRPTLHSLQAVDRALLRASAAEVNKLESRVPFLA
                     TTASITPFIGLFGTVWGVMIAFEGIGRTSSTNLAVVAPGIAEALIATAAGLFAAIPAV
                     YFYNALANRVKRLASLMDDFSLEFLNIAERNFT"
     gene            1087..2724
                     /gene="recN"
                     /locus_tag="PKK95_11850"
     CDS             1087..2724
                     /gene="recN"
                     /locus_tag="PKK95_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOC18958.1"
                     /translation="MLRHLAIQNLAVIEAAAVDFEPGLNVLTGETGAGKSILVEALGL
                     LLGARSSPDLVRTGADAASVQAVFDDPAGAELILGREVTVSGRSRAFLDGRLVPATSL
                     REAASRLVDLHGQHEHQALLDPDTHLDLLDRFAEVTAARAEVADCFAELRQARDELSR
                     ARQLEEQRAARTEIVEFQLAELGRVAPRPGEDAELASLRTLLASAERVRRLSDESYAL
                     LYDRDDAALGSLGQVWKRLAELAALDARFLPHLEQKDAIKAQLEDLAALLRGYAASLD
                     ASPARLQEVEDRLAALERLKRKHGPTLDDVLERHRALRAELKALGSAGERAAEAEQRL
                     AAATERYLRIAATLSDARRQAAGRFSRALERQLADLAMNDTRFEVRFADGHPGPERWT
                     ARGTDLAEFYASPNPGEELRPLARIASGGEISRVMLAIKNLASLDQPGKTLIFDEVDA
                     GIGGRVADAVGRKLRKLGESYQVLCITHLPQIAACGHAHYRIEKRVYRGRTTTRVERL
                     DRAGREEELARMIGGETVSAAAKANAREMLKTRSAPW"
     gene            2813..4165
                     /gene="miaB"
                     /locus_tag="PKK95_11855"
     CDS             2813..4165
                     /gene="miaB"
                     /locus_tag="PKK95_11855"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOC18959.1"
                     /translation="MKYLVETFGCQMNVHDSERIAGLLEQAGHEPADDEQDAELVILN
                     TCSVREHAAEKLFSRLGELRAVTGRHGQHPLLAVAGCVAQQEGERLLARVPYVDLVIG
                     TQAVRRLPDLVEALHRSGRQQVDVRAYEDPSFPLGIARRSDPVRAYVTIMEGCNDYCS
                     FCVVPYTRGHERMRRKAEILDEVAEAVAGGRREIHLLGQIVNHYQAPDDPACDFPALL
                     EAVDAVPGVQRIRFASPHPRHASDRLVEAIRDLPRVCKHLHLPVQSGSSRVLAAMRRR
                     HTREEYLALVGRLRDAVPSIAISTDMIVGFPGETADDFEETLSLVRQVRFQAMFSFKY
                     SQRPNTLAAKRLEDDVPEEEKGRRLWALQQEQREIQLALNRQMVGTEVEVLVDSLSRR
                     RTDEVSGRTTGNVVVNFAAPRSTIGSLVRVRITDAGANSVKGACSEPTVSWSAGGGQG
                     "
     gene            4202..4687
                     /locus_tag="PKK95_11860"
     CDS             4202..4687
                     /locus_tag="PKK95_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOC18960.1"
                     /translation="MQIEMTIKGLVVDPITNMPIIVLRDAEGQRVLPIWVGIFEANAI
                     ALRIENISTPRPMTHDLLRNVIEDLNGVVRKIVVSDLKDNTFYALIYVEVGGQTLAID
                     SRPSDAIALALRADAPIFVEETVLDNAKGIDFSTDKADSERLQKWLESLDPEELGKYK
                     M"
     gene            4801..5556
                     /locus_tag="PKK95_11865"
     CDS             4801..5556
                     /locus_tag="PKK95_11865"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOC18961.1"
                     /translation="MILAIANQKGGVGKTTTAINLGAALAFRGHKTLLIDLDPQANST
                     MSYTDLRDIHRSLYDVLAEDNCPLLDIVVPTSVPNLSIVPARIGLAKLEARLAGEVDA
                     HYRLKDRLEPAARVFDTIVVDCPPALGLLTVNALVAATHLIIPIQSSYYALEGTDDLL
                     ETVEKVRSRANPGLDLIGVLITMHDRRTSLARDIRGQIQKVFGPKVFKTVITKSVRLE
                     ESPAYRESIFTFAPNSSGATEYYSLCEEVMERA"
BASE COUNT          967 a         1945 c         1956 g          835 t
ORIGIN      
        1 cggcaggttg acgtcgagcc cgcgctgcat catcggcgcg gtgaccatga agatgatcag
       61 cagcaccagc atcacgtcca ccagcgggac cacgttgatc tcgctcagcg acgaggccac
      121 gcggcggctc cggccgcgcc cgctcccgcc gttcgtctcg atcggctgga ccttcggcat
      181 cggcctaggt gaagttcctc tccgcgatgt tgaggaactc gagcgagaag tcgtccatca
      241 gcgaggccag cctcttcacc cggttcgcca gcgcgttgta gaagtagacg gccgggatcg
      301 ccgcgaagag ccccgccgcc gtcgcgatca gcgcctcggc gatgccgggg gccaccacgg
      361 ccaggttggt cgacgaggtg cgcccgatcc cctcgaacgc gatcatcacc ccccacaccg
      421 ttccgaacaa gccgatgaac ggcgtgatgc tcgccgtcgt ggccaggaac ggcacccggc
      481 tctcgagctt gttcacctcg gccgcagagg ccctcaacag cgcccggtcc accgcctgca
      541 ggctgtgaag cgtcggccgg accgccgtcg ccccggtcac cgccgccccg gccggctgcc
      601 gaagctgggc gttcagctcc gcgtaccccg cctggaagat ccccaccagc gggctgtccc
      661 cgagcgtctt gcagaccgcc tgcacctcgg aaaacttgct gctcttgcgg aagacgtcga
      721 ggaaggtgga cgagtgccgc tccgcccgcc ggaacaccca gagcttgtag aggatgatgc
      781 tccaggacac cacggaaaag acgaggagga tcaggagaac gactttggcc gcgaggctcg
      841 accgcaggat cagttcgatg acgttgccgc ccagcccggg ttcgggcgcg gcagggccct
      901 gtacctggag ggcaaggacc aggtctccaa gcgtgcgcaa acgtgcctcc gcttcatggt
      961 ggacgcgccg ctgggacagc gcagttcgcc ctagcgtagc acgggggcga aaccctgtca
     1021 aaagcatgat acgattgcat ttcccggctt tctggcgtct tcgcccctgt ccctcgggga
     1081 gcccgcatgc tgcggcacct ggcaatccag aacctcgcgg tcatcgaggc ggccgccgtc
     1141 gatttcgagc ccggcctgaa cgtcctgacc ggcgagaccg gcgccggcaa gtcgatcctc
     1201 gtcgaggcgc tcggcctgct gctcggcgcc cgctcgtcgc ccgacctggt ccggacgggc
     1261 gccgacgccg cttccgtcca ggctgttttc gacgaccccg ccggggccga gctgatcctc
     1321 ggcagggagg tgaccgtctc gggccgcagc cgcgccttcc tcgacggccg gctggttccc
     1381 gccacctccc tccgggaggc cgcgagccgc ctggtcgacc tgcacggcca gcacgagcac
     1441 caggcgctgc tcgaccccga cacccacctc gaccttctcg accggttcgc cgaggtcacc
     1501 gcggcgcgtg ccgaggtcgc cgattgcttc gccgagctgc gccaggcgcg cgacgagctg
     1561 tcccgggcgc gccagctcga ggagcagcgg gccgcgcgca ccgagatcgt cgagttccag
     1621 ctcgccgagc ttggacgcgt ggcaccccgc cccggcgagg acgcggagct cgcgtcgctc
     1681 aggacgctgc tcgcgagtgc cgaacgggtg cgccgcctgt cggacgagag ctacgcgctc
     1741 ctctacgacc gcgacgacgc cgccctcggc tcgctgggac aggtgtggaa acggcttgcc
     1801 gaactggcag cgctcgacgc ccgtttcctg ccccacctcg aacagaagga cgcgatcaag
     1861 gcgcagctcg aggacctggc ggccctgctg cgcggttacg cggccagcct cgacgcctcg
     1921 cccgcccggc tgcaggaggt cgaggaccgg ctcgccgccc tcgagcgcct gaagcgcaag
     1981 cacggcccga cgctcgacga cgtgctcgag cggcaccgcg cgctgcgcgc ggaactgaag
     2041 gcgctcggct cggcagggga gcgtgccgcc gaggcggagc agcgcctggc cgccgccacc
     2101 gagcggtacc tgcgcatcgc cgccacgctg tccgacgcac gccgccaggc cgccgggcgc
     2161 ttctcgcgcg ccctcgagcg ccagctggcc gacctcgcga tgaacgacac gcgcttcgag
     2221 gtgcggttcg ccgacggcca ccccgggcca gagcgctgga cggcccgcgg caccgatctc
     2281 gcagagttct atgcttcacc gaaccccgga gaagagctgc ggcccctcgc gcgaatcgcc
     2341 tctggcggtg agatctcccg ggtgatgctg gcgatcaaga acctggcgtc gctcgaccag
     2401 ccggggaaaa cgctaatctt cgacgaggtc gatgccggaa tcggcggacg tgtcgccgac
     2461 gcggtgggcc ggaagctgcg gaagctgggg gagtcgtacc aggtgctgtg catcacgcac
     2521 ctgccgcaga ttgccgcgtg cggccacgcc cactaccgga tcgagaaacg cgtgtaccgc
     2581 ggccggacga caaccagggt cgaacggctc gaccgggccg gcagggaaga ggagctggcg
     2641 cggatgatcg gcggcgagac ggtttcggct gccgcgaagg ccaacgcgcg cgagatgctg
     2701 aagacccggt cggcaccgtg gtaggggcga aagcgataat aaggcgaaag gcgaaagcga
     2761 agttctggcg aaagcgaaag tagcggcggc ggggcatggc tcagggctgt acatgaagta
     2821 cttagttgag actttcggct gccagatgaa cgtccacgac tccgagcgaa tcgcggggtt
     2881 gctggagcag gcggggcatg aaccggcaga cgatgagcag gacgcggagc tggtgatcct
     2941 gaacacgtgc agcgtcaggg agcacgccgc cgagaagctg ttttcgcgcc tcggcgagct
     3001 gcgcgcggtc accggccgcc acggccaaca cccgctcctc gcggtcgcgg gctgcgtggc
     3061 ccagcaggaa ggcgagcgcc tgcttgcccg ggtcccatac gtggacctgg tgatcggcac
     3121 ccaggccgtc aggcgcctgc ccgaccttgt cgaggcgctc caccgctcgg gccggcagca
     3181 ggtcgacgtc cgggcgtacg aggatccaag cttcccgctc ggcattgccc gccgctcgga
     3241 tccggtgcgg gcctacgtca ccatcatgga ggggtgcaac gattactgct cgttctgcgt
     3301 ggtgccttac acccgcgggc acgagcggat gcggcgaaag gccgagattc tcgacgaggt
     3361 ggccgaggcg gtggccggcg ggcgccgtga gatccacctg ctcgggcaga tcgtgaacca
     3421 ctaccaggct cccgacgacc cggcctgcga tttcccggcc ctgctcgagg ccgtggacgc
     3481 cgttcccggg gttcagcgca tccgttttgc cagcccgcac ccgcggcacg ccagcgatcg
     3541 cctcgtcgag gccatccgcg acctgcctcg cgtctgcaag cacctgcacc ttccggtgca
     3601 atccggctcg tcgcgcgtgc tcgcggccat gcggcggcgg cacacgcgcg aggagtacct
     3661 ggcgctcgtc ggccggctgc gggacgccgt gccgtcgatc gcgatctcga ccgacatgat
     3721 cgtggggttt ccgggcgaga cggcagacga cttcgaggag acgctctcgc tggttcgcca
     3781 ggtgcgcttc caggcgatgt tctcgttcaa gtactcgcag cgcccgaaca cgctggccgc
     3841 caagcgcctc gaggacgatg tcccggagga ggagaaggga aggcggctct gggcgctgca
     3901 gcaggagcag cgagaaatcc agctggccct caaccgtcag atggtcggga cggaggttga
     3961 ggtcctggtg gactcactga gccggcggcg gaccgacgag gtctcgggcc gcacaacagg
     4021 gaacgtggtc gtgaacttcg cggcgccccg ttccacgatc gggagtttgg tgcgggtgcg
     4081 gatcacggat gcgggggcga acagcgtgaa aggggcgtgc agcgagccca ccgtttcctg
     4141 gtccgccgga ggcgggcagg ggtaagatgg gctcacgcag ccggcggaga ctggagcagc
     4201 catgcagatc gagatgacga tcaaggggct ggtggtggac cccatcacca acatgcccat
     4261 catcgtgctg cgagacgcgg aggggcagcg ggtgctcccg atctgggtgg gcatcttcga
     4321 ggcgaacgcg atcgccctga ggatcgagaa catctccacg ccgcggccga tgacccacga
     4381 cctgcttcgc aacgtgatcg aagacctgaa cggggtcgtc cggaagatcg tggtgtccga
     4441 cctgaaggac aacaccttct acgcgctgat ctacgtggaa gtgggcgggc agacgctggc
     4501 gattgactcg cgcccgagcg atgccattgc cctggccttg agggcggacg cgccgatctt
     4561 cgtcgaggag acggtgctcg acaacgccaa gggaatcgac ttcagcaccg acaaggccga
     4621 ctcggaacgg ctccagaagt ggctcgagag cctcgatcca gaggaactcg gcaagtacaa
     4681 gatgtgaggg ccccggccga gggctcattt tgccgcggcg ccgcccaacc gtttgacacg
     4741 cccccccagc gtctctagaa tagcgctgcc tcacccgcgc acacgctgac gctacgcctc
     4801 atgatcctcg caatcgccaa ccagaagggg ggcgtgggga agacgaccac ggcgatcaac
     4861 ctcggggccg ctctggcctt ccggggccac aagacgctgc tgatcgatct cgatccccag
     4921 gccaactcca cgatgtccta cacggacctg cgggacatcc accgcagcct gtacgacgtt
     4981 cttgccgagg acaactgccc gctcctcgac atcgtcgtgc cgacctccgt gccgaacctg
     5041 tcgatcgttc cggcgcggat cgggctggca aagctcgagg cgcggctggc gggggaggtg
     5101 gacgcgcact acaggctgaa ggaccgcctc gagccggccg cacgcgtctt cgacacgatc
     5161 gtggtggact gtccaccggc gcttgggctg ctgacggtga acgcgctggt tgcggccacg
     5221 cacctgatca tcccgatcca gtcgtcgtat tacgcgctcg agggaaccga cgacctcctc
     5281 gagactgtgg aaaaggtgcg aagccgggcc aaccctgggc tcgatctgat cggggtgctg
     5341 attaccatgc acgaccgccg gacgtcgctg gcgcgggaca tccgcggcca gattcagaag
     5401 gtcttcggcc cgaaggtgtt caagacggtc atcaccaaga gcgtgcgcct cgaggaaagc
     5461 ccggcgtacc gggaatcgat cttcacgttc gcgccgaact cgtcgggcgc gaccgagtac
     5521 tattcgctct gcgaggaggt catggaacgt gcctaggcga gggttgcccg agacgattcg
     5581 gatgcgtcac gacgagcatt acgtcgaggc cctgaccgcc ggcgtgggag cgccgatcgg
     5641 ccggatggtc ccgatcgagc aggtgagccc gaacccgtcg caaccgcggc aggtgatggg
     5701 cga
//
LOCUS       DAOPQX010000228         5687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15536_length_5687_cov_4.291016, whole genome shotgun sequence.
ACCESSION   DAOPQX010000228 DAOPQX010000000
VERSION     DAOPQX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15536_length_5687_cov_4.291016"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="PKK95_11870"
     CDS             <1..1365
                     /locus_tag="PKK95_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOC18962.1"
                     /translation="RYEIASVALEDYVAGVVAAEAPPASPEAASKALAIVARTFALAN
                     RERHGAEGFDLCDLTHCQAVTTPTASSRAAARATGGLVLRFRGELAQVFHSASCGGHS
                     ERPSAVWPGAIDPPYLVSRPEPACRLDSTWTTDVSAAQVTAALRAAGLRGEALRSLRV
                     AARTPSGRVAAVEAGGFDPGVISAESFRLSLGRTAGWHLLKSTAFEIQRRASGYRFTG
                     HGRGHGVGLCMEGAVRLARQGVGAAAILRHYFPGLDADMPSARGTVEPGTRSSIQVFV
                     PSGEEAAREPIRRMAREALAAVSAAANLPGPASIRLVFHPTVQAYARATGLPWWTAGA
                     ARGESIDLLPPAALRRRGLLERTIRHEIAHAVIGSRLAGRPLWIQEGAAMFLAGELDE
                     VERRGTPDGRANVRCPGDAEITGASSADALKQAYRRAAACFARELRAGRRWNEIGEVS
                     TSTR"
     gene            complement(1346..3475)
                     /locus_tag="PKK95_11875"
     CDS             complement(1346..3475)
                     /locus_tag="PKK95_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOC18963.1"
                     /translation="MLLRRSSRGTPDGSSGDVLVAADVRRDEIARRAAQVMADRAGYS
                     QFVLRLDRFARTVLLRNPGLSPERRASLERPSYLFLSDRQGGFPVEGFWLEEPGGTVR
                     QMPETPFVDLVVEEGDFDPSDNDGLVAIHAHELGHLIMGALAGPAPRRASTGVHFTTV
                     RTDGWLAFVEGWGEHFQPMAVDHHRKKASGEDHRRDACATDHRRNAGATDHRRDACAT
                     DHRRNAHGDETSAPPERVWYDRFAREQIEGCGICPANLRFLRWQGPGEQRLREEGVRS
                     NLFIHDLVLPPALAAGSRPPFEARMYRDVMPPSSDGPLKSGARALASEGLVATFFHRL
                     ASDERLRRSYREPAFYAPFLPDTQAGPLVSLGPSALISPEENAYLKIFHVLHHAFSWG
                     DWPLVELVKGYAATFPDEAAIVYDVFLDVTRGVTVERAAFSRHADPGYLPWLRERLLS
                     GEVRLDGNLGRPLWLVVPRVSLGMGVYRYFLVPSPFTFDLNAADAADLRSVPGVDAAL
                     AASIVKARDERGAFDRVEDLASVPGMTPELVSRFVSMRERMQARLDEREWQGSNPTWV
                     MNWLVRLLKGSYHLAAAWQLGRALVLAALLYLLTSVTIGAAFSRGVNRSGAAARGGRL
                     GSLRRLVRSLARGLAVAVWPLAGSVALYAAGILPGPANMTAVGAAVGLAIVGLRRATG
                     SAAPGRFAALAPVAGSIAAAILIGSMY"
     gene            complement(3636..4388)
                     /locus_tag="PKK95_11880"
     CDS             complement(3636..4388)
                     /locus_tag="PKK95_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18964.1"
                     /translation="MKVRIATGAGWLAGAHALILGLFWLLLRVPESNLLMLAASAVLA
                     ATIVVASGCVETVALLALRGGPFGRQLLRRGVGGVLPFVASLAVFALVWFATVRFDGW
                     WASARGEIDAWLIARLGWTDAGWLHAAVRWATAFVRWVLGLSLALALFAALVREEPAG
                     EARAEDGGMRGGLGRRWFRAAVSPRALLGLLALIVVFAWLPWQAAYWRPSWVSPSWAE
                     VLFVATKLGALYLLATIGWTLALLLANRLRLP"
     gene            complement(4385..>5687)
                     /locus_tag="PKK95_11885"
     CDS             complement(4385..>5687)
                     /locus_tag="PKK95_11885"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18965.1"
                     /translation="WWKTAEPDVFMTAYALHGLVETRRAGYSVEQWRVASAAGALRAL
                     MQRNPRALADLKAYVAHVLAAADPEGGAAAAIDEAWAARDGASPAGWGLLALTLGARR
                     DPRGDAAAQALESTVQRRGDLAWWPSDRDTLLGDVGDASVEATAFAVRALAARDPRHP
                     LIEPAVRWIVLNRAQGTWWSSTKQTAMVLYALLDVMRARREHGAPVEAEVLVNGESAG
                     RVRFDAASMTAPDPARIVVPALGGANTIVVRAKGEGTVYWSAAALSYDRAGASEPAGA
                     RKLALARRYFLLESVRRDDRVVYRERPLAGPVRPGDLVLVRLTAAGSPDWRYLLVEDP
                     IPAGTEPIQLAELYPLERRRPRFDPARREYRDDRVALFQENFLQGRYDYEYILKVTTP
                     GTFGAMPARIVPMYVPGVQASTAAERLIVEAAADRSQGAGR"
BASE COUNT          893 a         2048 c         2008 g          738 t
ORIGIN      
        1 cggtacgaga tcgcctcggt tgctctcgag gactacgtgg caggggtcgt cgcggccgag
       61 gcgcccccgg cgtctccgga ggcagcatcg aaggcgctgg cgatcgtcgc ccgcaccttc
      121 gcgctggcca accgggagcg ccacggcgcc gagggcttcg acctctgcga cctgacgcac
      181 tgccaggcgg tgacgacgcc cacggcgtcc tcgcgcgcag cggcccgggc caccggcggc
      241 ctggtgctgc gatttcgtgg tgagctggcg caggtgttcc acagcgcttc ctgcggcggg
      301 cactcggagc gtccctccgc ggtgtggcct ggcgcgatcg acccgccgta ccttgtctct
      361 cggcccgagc cggcctgccg tctcgactcc acctggacga ccgacgtgag cgccgcgcag
      421 gtgaccgcgg ccctgcgagc ggcaggcttg cggggcgagg ctctgcgatc gctgcgggtg
      481 gccgcgcgca cgccgtcagg ccgggtagcc gccgtcgagg ccggcggctt cgaccctggc
      541 gtcatctcgg ccgagtcgtt ccggctttcg ctggggcgga cggccgggtg gcacctgctg
      601 aagagcaccg cgttcgagat ccagcgacgg gccagcggct accgcttcac cgggcacggg
      661 cgggggcacg gcgtcggctt gtgcatggag ggggcggttc gcctcgcgag gcaaggcgtt
      721 ggggccgccg ccatcctgcg gcactacttc ccgggcttgg atgccgacat gccgagcgcc
      781 cgtgggaccg tcgagccagg cacgcgctcc tcgattcaag tgttcgtgcc atccggggaa
      841 gaggcggcgc gggagcccat tcgacggatg gcgcgggagg ctctcgccgc ggtgtccgcc
      901 gcggcgaacc tgccggggcc cgcctccatc cgcctcgtct tccacccaac cgtgcaggcc
      961 tatgcgcgcg cgaccggcct cccgtggtgg acggcgggcg ccgcgcgcgg cgagagcatc
     1021 gacctgctgc cacccgctgc gctgcgccgc cgcggcctgc tcgagcgaac gatccgccac
     1081 gagatcgcac acgccgtcat cggcagtcgc ctggcgggcc gacccctgtg gatccaggag
     1141 ggcgcggcca tgttcctggc cggagaactt gacgaggtag agaggcgcgg cacgccggac
     1201 ggccgcgcga acgtccgttg cccgggcgac gccgagatca ccggcgcctc gtctgcagac
     1261 gcgctgaagc aagcctaccg ccgtgccgca gcctgcttcg cccgcgagct gcgggccgga
     1321 cggcggtgga acgagatcgg cgaggtcagt acatcgaccc gatgaggatt gccgcggcaa
     1381 tcgagcccgc caccggcgcc agggcggcga acctgccggg tgccgccgaa ccggttgcgc
     1441 gtcgaaggcc cacgatggcc aacccgacgg cggcgccgac cgcggtcatg ttcgcggggc
     1501 cgggcaagat cccggccgcg tagagcgcca cgctgccggc caggggccag acggcgaccg
     1561 ccaggcccct cgcaagagag cggacgagcc ggcgcaacga gccgagccgt cctccgcgtg
     1621 cggccgcgcc ggaacgattg acccctcgag agaaggctgc gccgatcgtc acacttgtca
     1681 gcagatagag cagcgcagcc agcacgagtg cccgcccgag ctgccatgcg gccgccaggt
     1741 ggtacgagcc cttcaacagg cggacgagcc agttcatcac ccaggtcggg ttcgaccctt
     1801 gccattcccg ttcgtcgagc cgggcctgca tccgctcgcg catcgagacg aagcgcgaga
     1861 cgagctctgg cgtcatcccg ggaaccgacg ctaggtcctc gacccgatcg aacgcgcccc
     1921 gctcgtcccg cgccttcacg atcgacgccg cgagcgccgc gtccacgccg ggcaccgacc
     1981 gaaggtcggc ggcgtctgcg gcattgaggt cgaaggtgaa cggcgaggga accaggaagt
     2041 agcggtagac gcccatcccg agggagaccc gcggcacgac gagccacaac ggccggccga
     2101 ggttcccgtc gagccgcact tctcccgaca gcaggcgctc gcgaagccac ggcaggtagc
     2161 ccggatcggc gtgcctcgag aaagcggcgc gctccacggt gaccccccgc gtcacgtcga
     2221 ggaaaacgtc gtagacgatc gccgcctcgt ccgggaacgt cgccgcgtac cccttcacca
     2281 attcgacgag cggccagtcg ccccagctga acgcgtggtg caggacgtgg aagatcttca
     2341 ggtaggcgtt ctcctcgggc gagatcagcg ccgaagggcc caggctgacg agggggccgg
     2401 cctgagtgtc gggcaggaaa ggcgcgtaga acgccggctc ccgatagctt cgtcgaagcc
     2461 gctcgtccga ggcgaggcgg tggaagaagg tggcgacgag cccctcgctg gccagcgcgc
     2521 gggcgccgct cttcagcggg ccgtccgaag acggcggcat gacgtcgcga tacatccggg
     2581 cctcgaacgg aggccggctg ccggcagcga gcgcgggcgg gagcacgagg tcgtggatga
     2641 acaggttcga ccgcacaccc tcttcgcgca ggcgctgttc gccgggtccc tgccacctga
     2701 ggaaccgcag gttcgcggga cagatcccgc agccctcgat ctgctcccgg gcgaaccggt
     2761 cgtaccacac tcgttccgga ggtgccgatg tctcgtcgcc atgggcgttt cgcctgtgat
     2821 cagtggcaca ggcgtctcgc ctgtgatcag tggcaccggc gtttcgcctg tgatcagtgg
     2881 cacaggcgtc tcgcctgtga tcctcgccac ttgccttctt gcgatggtgg tccaccgcca
     2941 tcggctggaa gtgctcgccc cacccctcca cgaatgcgag ccaaccgtcg gtcctcaccg
     3001 tcgtgaagtg aacgcccgtc gaggccctgc gtggagccgg tccggccagc gctcccatga
     3061 tcaggtggcc gagctcgtgc gcgtggatcg cgacgagacc atcgttgtcg gatggatcga
     3121 agtcaccctc ttccacgacg agatccacga acggtgtttc cggcatctgc ctgaccgtgc
     3181 cgccgggttc ctcgagccag aagccctcca cgggaaagcc gccctgccga tccgacagga
     3241 acaggtagga cggccgttcg agcgaggcac gccgctcggg cgacaggccg gggttgcgca
     3301 acaggacggt tcgcgcaaag cggtcgagcc ttaggacgaa ctgggagtag ccggcgcgat
     3361 cggccatcac ctgcgcggcg cgtcgcgcga tctcgtcgcg ccgcacgtcg gcggccacca
     3421 gcacgtcgcc gctcgacccg tccggtgtgc ccctgctact cctcctcagg agcacgatgg
     3481 gaaccgaatc ccccgtgtcg ggaagctggc cggcggcagg gcatggaagt gacaggagga
     3541 cagcgaggag ggcgatgagg cgcacgacat ctaggacgca gtgaaagagg gaagagttcc
     3601 gtgcggagcg aacattgtca atagtgagcg ccgtgtcagg ggaggcgcag gcggttggcg
     3661 agcaacaggg cgagcgtcca gccgatcgtc gcaaggaggt acagggcgcc cagcttggtg
     3721 gccacgaaga gaacctcggc ccacgagggc gagacccagc ttggacgcca gtaggccgcc
     3781 tgccacggga gccaggcgaa cacgacgatc agcgcgagca ggcccagcaa agcacgcggc
     3841 gagacggctg cgcggaacca gcgacgtccc agtccgcccc tcatgccgcc atcttccgct
     3901 cgcgcttcac ccgccggctc ttcccgcacc agcgcggcga acagcgcgag cgccagcgag
     3961 agccccaaca cccagcgaac gaaggcggtg gcccacctca cggctgcgtg cagccagccg
     4021 gcgtcggtcc aacccaggcg cgcgatcagc caggcatcga tctctccgcg cgccgacgcc
     4081 caccatccat cgaagcgcac ggtcgcgaac cagacgaggg cgaacacggc gagggacgcg
     4141 acgaagggca gcacgccccc gacgccgcgc cgaagcaatt gccgcccgaa tggcccgccc
     4201 cgcagcgcga gcagggcgac ggtctcgacg cagcccgacg ccacaacgat ggtcgcggcc
     4261 aggacggccg aagccgccag catcagcagg ttcgactcgg ggacgcgaag gagcagccag
     4321 aacaacccca gaatgagcgc gtgtgctcct gccagccatc cggcgccggt cgcgatccga
     4381 accttcatcg ccctgctccc tggctgcgat ccgcggccgc ctcgacgatc agtctttccg
     4441 ccgccgtgga cgcctgcacg cctggcacgt acatcgggac gatccgcgcc ggcatcgcac
     4501 cgaacgtgcc gggcgtcgtc accttcagga tgtactcgta gtcatagcgg ccctgcagga
     4561 agttctcctg gaagagggcg acgcgatcgt cgcgatactc ccggcgcgcc gggtcgaagc
     4621 gcggccgtcg ccgctcgagc gggtacagct cggccagctg gatcggctcg gtgcccgcgg
     4681 ggatcgggtc ctcgacgagc aggtagcgcc agtcgggcga gcccgcggcg gtgagccgca
     4741 cgaggacgag atcgccgggc cggaccggtc cggcgagagg gcgctcccgg tagacgacgc
     4801 ggtcatcccg gcgcacggac tcgaggagga agtatcggcg cgcgagcgcg agctttctcg
     4861 ccccggccgg ctcagacgcg cccgctcgat cgtagctgag cgcggcggcc gaccagtaca
     4921 ccgtgccttc gcccttggcg cggacgacga tggtgttcgc gccgccgagc gccggtacga
     4981 cgatcctggc cggatcgggc gcggtcatcg acgccgcgtc gaagcggacc cgtccggcgc
     5041 tctcgccatt caccagcacc tcggcctcga ccggggcgcc gtgctcgcgt cgtgcacgca
     5101 tgacgtcgag cagcgcgtag agcaccatcg cggtttgctt cgtgctcgac caccaggtgc
     5161 cctgcgcacg gttcagcacg atccaccgga ctgccggctc gatcagcggg tgccgtggat
     5221 cgcgggcggc gagtgcgcgc accgcgaacg cggtggcctc gacgctggcg tcgccgacgt
     5281 cgccgagcag cgtgtcgcga tccgacggcc accaggccag gtccccgcgg cgctggaccg
     5341 tcgattcgag cgcctgcgcg gcagcgtcac cgcggggatc gcgccgcgcg ccgagcgtga
     5401 gcgcgagcag cccccagccg gccggcgacg ccccgtccct ggcggcccac gcctcgtcga
     5461 tcgcggcggc cgcaccgccc tcgggatccg ccgccgccag cacgtgcgcg acgtaggcct
     5521 tcaggtcggc cagcgcccgg ggattgcgct gcatcaacgc gcgaagcgct ccggcagccg
     5581 acgcgacgcg ccactgctcg accgagtagc cggcccggcg ggtttcaacg aggccgtgca
     5641 gcgcgtacgc ggtcatgaac acgtcgggct ccgccgtctt ccaccag
//
LOCUS       DAOPQX010000229         5677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15572_length_5677_cov_5.080754, whole genome shotgun sequence.
ACCESSION   DAOPQX010000229 DAOPQX010000000
VERSION     DAOPQX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15572_length_5677_cov_5.080754"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKK95_11890"
     CDS             <1..495
                     /locus_tag="PKK95_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOC18966.1"
                     /translation="EFSIGDGRRFMLHGRIDRINRLKGHDYEVADYKGRYRRDDWQGA
                     FAGGTRLQHALYGVAASALLKRIDVRARVVRGTYLFPSVRGFRARKIIPAPSREKLAA
                     VLRDLSDVIGSGCFAPANGKQACQWCEYGAACHVADPEVVQAKVEDEKDAALAAYWRL
                     RSHE"
     gene            488..3760
                     /locus_tag="PKK95_11895"
     CDS             488..3760
                     /locus_tag="PKK95_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC18967.1"
                     /translation="MSEAPTPSDQPTRDRIVSDFGRNFLVEAGAGSGKTYSLAMRMAA
                     GVERGAFEVDGMAAVTFTRKAAAELRGRFQEALEDRLRCAPGDDERRRLRGALDGIER
                     LFAGTIHAFCAHLLRERPVDAQVAPCFEELGDADNVLLRKQAWRDFLVERRAAGWRPL
                     LDLFEAGVRPWELDGGIASVCEHEDVEFDMGSGEPPEAAPVWKEVDRFWARLEALAPP
                     EFPEETTCPLQRRFDEYDGRIRLARRDRVASLAEFCKAWKRDVVQKHWAGRGKQVKTL
                     VDDFTSTVVEPFLEAWRAYVHHLAMIVLADARASYAEVRRRRNVVNYVDLLRVTARML
                     RERRAVRRALQAKYRWLFIDEFQDTDPIQAEIFLMLAAAEAASPGNGDGGTAVDPFAL
                     PLRPGSLFVVGDPKQSIFRFRRADIDIYNRVGDRIVVTGGERLLLTANFRSLPGVCAL
                     ANQVFPPLFGKLSLPWSPPFQPLHPVRPEAEGGPGPTVAKLTLPETKKAPEATRQEAA
                     AIARYIKGEMAAGRRKAGDFLVLTRFRPRLAEYVAALDALDVPVEVSGAGFFCKSPEV
                     QALAGLLAALADPLDAVSLVGVLRGPLFGISDPELFRFRQAGGRFELHAVLPEAADER
                     EAAALDQRFGPVLPAMRRLQALHRLSWSMPLGAALDAMLEETGWLALASTGPGGAPAG
                     HLLQAIDRVREVVERGGGLADAAEALQEDEQSSEADALPLEPGQRDVVRLMNLHKAKG
                     LQAPVVILADAAHDHRFSVTLRVRRDDPCGRPRGVLCISSRSNYGYGDVLVGRPRDWA
                     VHEAEEEKYLDAERLRLLYVAATRAEDLLIVCRSEKQKANKAWKEFEAFLEGVPEIVM
                     PPDPAPAKAAEGDVSPKARMAARRLREEEHSLAREASWAVTRVTGHHAVPSEAPGAEP
                     AGDLGVPSEARSAESRDEAGGVLWGTLVHGLLEHAVQYAHASRGDLERLARWLTVETP
                     ALRPLVGLAVDLVEQARKAPFWASVLEAPEAHVEVPFAVRVEATPTVVRGVIDLAYRT
                     QTGWCLLDYKTDRSPGDEAAVRARHGAQVRQYAAAWKHASGEGVAASGIVLVRTGQVV
                     ET"
     gene            complement(3742..4701)
                     /locus_tag="PKK95_11900"
     CDS             complement(3742..4701)
                     /locus_tag="PKK95_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18968.1"
                     /translation="MALLERYLDAVGRHLPPAEEEDILAELRDDIQAKYDRRAEELGR
                     ALTQDDEAELLRPYGRPIVLASRYRKPQHLVGPAWFPFYWNTLKIALAVALIVAVGLP
                     LVLALMGRSPGAIVRALFNFPETALTVFAWVTIVFAAVEAFTSSTKFRDNWDPRSLPR
                     PRPAGVARTSRFDVAIELGVSAAFLGWWTAAHRSPDLLFLPPVVALGPWWASVYLPLA
                     ALLLVSIVVKAVILVRPDWTGFRLAAGLLTTAAGTAIALFVAWSEPLLTGSGAGGEKL
                     AGILESLLRWSIGVAVVIAVTTTALEAWRYLRARRRTGQVSTT"
     gene            complement(4710..5069)
                     /locus_tag="PKK95_11905"
     CDS             complement(4710..5069)
                     /locus_tag="PKK95_11905"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOC18969.1"
                     /translation="MSRTADGTPAPPDQGEVFENLRQELRRGTLVLAVLSELRHERYG
                     YTLRSRLAQAGVELDEGTLYPLLRRLESQGLLASEWREEDNRRKRFYRLSPAGRAVLK
                     QLAAEWQSINTSLTRML"
     gene            complement(5219..>5677)
                     /locus_tag="PKK95_11910"
     CDS             complement(5219..>5677)
                     /locus_tag="PKK95_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HOC18970.1"
                     /translation="LTPPDRRRLEALQRWANLLDAAFGIPGTGIRFGIDALVGLIPGA
                     GDALAGLFSATILLQGARFGVPRLVLVRMVFNALVDVVVGAVPFLGDLFDVGWKANLR
                     NVRLLDRFVVQGQRTPTRGDYLFVTILLVILVAITALPLVALTLLVAYLT"
BASE COUNT          866 a         1898 c         2134 g          779 t
ORIGIN      
        1 gagttctcga tcggcgacgg gcggcggttc atgctccatg gccgaatcga tcgcatcaac
       61 cggctgaagg ggcacgacta cgaggttgcc gactacaagg gccgctaccg gcgcgacgac
      121 tggcagggcg cgttcgccgg aggcaccagg ctccagcacg cgctctacgg ggtggccgca
      181 agcgcgctgc tgaagcgcat cgacgtacgc gcccgggtcg tccgcggcac ctacctcttt
      241 ccgtcggtgc gcggcttccg cgcgcggaag atcatccccg caccctcacg ggagaagctg
      301 gccgcggtgc tgcgggacct ctccgacgtg atcggctccg gctgtttcgc gccggccaac
      361 ggaaaacaag cgtgccagtg gtgcgagtac ggtgcggcct gccacgtggc ggaccccgaa
      421 gtggtgcagg ccaaggtcga ggatgagaaa gacgcagccc tggcggcgta ttggaggctc
      481 aggtcccatg agtgaagcgc cgacgccctc cgaccagccg acccgcgacc ggatcgtgtc
      541 tgacttcggc cgcaacttcc tcgtcgaggc cggcgccggg tcgggcaaga cgtactcgct
      601 ggcgatgcgc atggcggccg gcgtcgagcg cggcgccttc gaggtggacg ggatggcggc
      661 cgtcacgttc acgcggaagg ctgccgccga gctgcgcggg cggttccagg aggcgctgga
      721 ggatcggctg cggtgcgcgc cgggggacga cgagcggcgg cggcttcggg gcgcgttgga
      781 cggcatcgag cgcctgttcg ccggcaccat ccacgcgttc tgcgcgcacc tgctgcgcga
      841 gcggccggtg gacgcgcagg tggctccctg cttcgaagag ctgggcgacg ccgacaacgt
      901 cctgctccgc aagcaggcgt ggcgcgactt cctcgtggag cggagggccg cgggatggag
      961 gccgctgctc gacctgttcg aggctggcgt ccggccctgg gaactggacg gcgggatcgc
     1021 cagcgtgtgc gagcacgagg acgtcgagtt cgacatgggc agcggggagc cgcccgaggc
     1081 cgccccggtc tggaaagagg tggatcgctt ctgggcccgc ctcgaggcgc tcgcgccgcc
     1141 agagttcccg gaggagacca cgtgcccgct gcagcggcgc ttcgacgagt acgacggccg
     1201 catccgcctc gccaggcgcg accgggttgc atcgctcgcg gagttctgca aggcgtggaa
     1261 gcgcgacgtg gtccagaagc actgggcagg ccgcggcaag caggtcaaga cgctggtcga
     1321 cgacttcacc tcgacggtgg tcgagccttt cctcgaggcc tggcgcgcgt acgtccacca
     1381 cctggcgatg atcgtgctgg cggacgcgcg cgcctcgtac gccgaagtcc ggcgccggcg
     1441 gaacgtcgtg aactacgtgg acctgcttcg cgtgacggca aggatgctgc gcgagcgccg
     1501 ggccgtccgg cgggcgctgc aggcgaagta ccggtggctc ttcatcgacg agttccagga
     1561 caccgatccg atccaggcgg agatcttcct gatgctcgcc gccgccgagg ccgcgtcgcc
     1621 cggcaacggc gacggcggca cggccgtcga tccgttcgcg ctcccgcttc gacccggctc
     1681 gctgttcgtc gtcggcgatc ccaagcagtc gatcttccgc ttccgccgcg ccgacatcga
     1741 catctacaac cgcgtcggcg accgcatcgt ggtgacgggg ggcgagcggt tgttgctgac
     1801 ggcaaacttc cgctcgctgc cgggcgtctg cgcgctggcc aaccaggtgt tcccgccgct
     1861 gttcggcaag ctcagcctgc cgtggtcgcc tcccttccag cctcttcacc ccgtgcgtcc
     1921 cgaggcagag ggcggcccgg ggccgaccgt cgcgaagctg acgctgcccg agacgaagaa
     1981 ggcgcccgag gcgacgcggc aggaagcggc cgcgatcgca cgctacatca agggcgagat
     2041 ggccgcgggg cggcgcaagg cgggcgactt cctcgtgctc acgcggttcc gtccgcggct
     2101 ggcggaatac gtggcggcgc tcgacgcgct cgacgttccc gtcgaggtga gcggcgccgg
     2161 gttcttctgc aagtcaccgg aggtgcaggc gctcgcgggg ctgctcgcgg cgttggccga
     2221 cccgctcgac gccgtgtcgc tggtcggcgt gctgcgaggc ccgctgttcg gcatcagcga
     2281 cccggagctg ttccgattca ggcaggccgg cgggcgcttc gagctgcacg ccgtgctgcc
     2341 ggaagccgcc gacgagcgcg aggcggccgc cctggaccag cggttcggcc ccgttctgcc
     2401 ggcgatgcgg cggctgcagg cgctccaccg gctctcgtgg tcgatgccgc tcggcgcggc
     2461 gctcgacgcg atgctcgagg agaccggctg gctcgcgctg gcctccacgg ggccgggcgg
     2521 agcgccggct ggccacctgc tgcaggcgat cgaccgcgtt cgcgaggtcg tggagcgggg
     2581 cggcgggctg gcggacgcgg ccgaggcgct gcaggaggac gaacagtcga gcgaggccga
     2641 cgccctgccg ctcgagccgg ggcagcgcga cgtcgtccgg ttgatgaacc tgcacaaggc
     2701 gaaggggctc caggcgcccg tcgtcatcct tgccgacgcg gcgcacgacc acaggttctc
     2761 cgtgacgctt cgcgtccgcc gggatgaccc ttgtggccgc ccgcgcgggg ttctctgcat
     2821 ttcttccagg tccaattatg ggtatggcga cgtgctggtg ggcaggcccc gcgactgggc
     2881 ggttcacgag gcggaggagg agaagtacct ggacgccgag cggctgcgcc tgctctacgt
     2941 ggcagcgacg cgggccgagg acctgctgat cgtctgccgg tcggagaagc agaaggccaa
     3001 caaggcgtgg aaggagttcg aggccttcct ggagggcgtt cccgagatcg tcatgcctcc
     3061 cgatcccgct cctgccaaag ccgccgaggg cgacgtctca cccaaggccc gcatggcggc
     3121 caggcgcctg cgcgaggagg agcacagcct ggcgcgcgag gcgtcctggg cggtgacacg
     3181 cgtgacgggc catcacgcag tcccgagcga ggcgccaggc gccgagccgg caggcgacct
     3241 cggagtcccg agcgaggcgc gaagcgccga gtcgagggac gaggccggcg gcgtcctctg
     3301 ggggacgctg gtgcacgggc tgctcgagca cgccgttcag tacgcgcatg ccagccgggg
     3361 cgacctggag cgccttgcgc gctggctgac ggttgagacg ccggccctgc gcccgctggt
     3421 gggcctggcg gtcgacctgg tggagcaggc ccggaaggcc ccgttctggg caagtgtgct
     3481 cgaggcgccc gaggcgcacg tcgaagtgcc gttcgccgtc cgagtcgagg caacacccac
     3541 cgtcgtccgc ggcgtgatcg atctcgcgta ccgcacccaa accggctggt gcctcctcga
     3601 ctacaagaca gaccgctcgc cgggtgacga ggccgcggtg cgcgcccggc acggagcgca
     3661 ggtgcgccag tacgccgcgg cctggaagca tgccagcggt gagggcgtcg ctgccagcgg
     3721 catcgtactc gtgcgcacgg gtcaggtggt cgaaacctga cccgtccggc gcctcgcccg
     3781 caggtagcgc cacgcctcga gcgccgtcgt tgtcacggcg atgacgacgg ccaccccaat
     3841 cgaccagcgg agcagcgact cgaggatgcc cgcgagcttc tctcccccgg ccccggaccc
     3901 cgtcaggagc ggctctgacc acgccacgaa caaggcgatc gccgttccgg cggccgtggt
     3961 caacagtccc gcggccagcc gaaaacccgt ccagtcgggc cgcaccagga tcacggcctt
     4021 gacgacgatc gacaccagca gaagggcggc aagcggaagg tagacgctcg cccaccacgg
     4081 cccgagcgcc acgaccgggg gcaggaagag caggtcgggg gagcggtgcg cggcggtcca
     4141 ccagccgagg aaggccgccg acacccccag ctcgatcgcg acgtcgaacc gggatgttct
     4201 cgcgacgcct gcgggccgcg gccgcgggag agagcgagga tcccagttgt cgcggaactt
     4261 cgtgctcgag gtgaaggcct cgaccgcggc aaagacgatg gtcacccagg cgaacaccgt
     4321 cagggcggtc tcggggaagt tgaagagcgc gcggacgatc gctcccgggg atcgtcccat
     4381 gagcgccagc accaggggaa gcccgacggc gacgatcaac gccaccgcga gcgcgatctt
     4441 cagcgtgttc cagtagaacg gaaaccaggc cggccccacc aggtgctgcg gcttccggta
     4501 gcgcgacgcc aggacgatcg ggcgcccgta cggccgcagc agctcggctt cgtcgtcttg
     4561 ggtgagagcg cggcccagtt cctccgcccg ccggtcgtac ttcgcttgga tgtcgtcgcg
     4621 cagctcggcc aggatgtcct cctcctcggc gggaggaagg tggcggccga cggcgtcgag
     4681 gtatcgttcg agcagggcca tggcgactcc tacagcatcc gggtgagcga cgtgttgatg
     4741 gactgccact cggcggcgag ctgcttgaga acggcgcggc cggcagggga gagcctgtag
     4801 aagcgcttcc gccggttgtc ctcctcgcgc cactcgctcg caagcagccc ctggctctcg
     4861 aggcggcgga gcagtgggta gagcgtccct tcgtcgagct ccacgcccgc ctgtgccagc
     4921 ctcgagcgca gcgtgtagcc gtagcgctcg tggcgaagct cggagaggac ggcgagcacg
     4981 agggtgcctc ggcggagctc ctgcctgagg ttttcgaaga cctcgccctg gtcgggcggc
     5041 gccggtgtgc catcagcagt gcgagacata gtatgtgata cacagtatac gaagcacagt
     5101 aaggtgtcaa gagctagggg ctaggggcta ggggctaggg gctgggggct gggggctagg
     5161 gcgcctgggc cggggcccgg gcacaggctg tgaacggcac tcggggcgcc ggggccggct
     5221 aggtcaggta cgcgaccagc agcgtcagcg cgaccagggg tagagcggtg atcgcgacga
     5281 gaatcaccag gaggatggtg acgaagagat agtcgccgcg cgtcggcgtg cgctggccct
     5341 gtacgacgaa gcggtcgagc agccggacgt tgcgcagatt cgccttccag ccgacgtcga
     5401 agaggtcgcc gaggaagggg acggcgccga cgaccacgtc gacgagcgcg ttgaagacca
     5461 tccggacgag gacgaggcgc gggacgccaa acctggcgcc ctgcaagagg atcgtggccg
     5521 agaagaggcc ggcgagcgcg tcgccggcgc cggggatcaa cccgaccagc gcgtcgatgc
     5581 cgaagcggat gccagtgccc gggatgccga atgcggcgtc cagcaggttg gcccagcgct
     5641 gcagcgcctc gaggcggcgg cggtcgggcg gggtgag
//
LOCUS       DAOPQX010000230         5674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15579_length_5674_cov_4.639616, whole genome shotgun sequence.
ACCESSION   DAOPQX010000230 DAOPQX010000000
VERSION     DAOPQX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15579_length_5674_cov_4.639616"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..519)
                     /locus_tag="PKK95_11915"
     CDS             complement(4..519)
                     /locus_tag="PKK95_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18971.1"
                     /translation="MDQTPRTAAVVADPSDITWTHVIYALHAFGVVLGVATSAAIVTS
                     FLFGLPSIVAVIINYVRRSRVRGTFLESHFRWQIRTFWYALGWIVLATVVSVPLMLDR
                     NQDAITAMLASFGALLNNPGPMLVWAALIVILTAIGFATFNIGLVVLMPIIGHATWHA
                     YRDLIEPLPPR"
     gene            730..1713
                     /locus_tag="PKK95_11920"
     CDS             730..1713
                     /locus_tag="PKK95_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOC18972.1"
                     /translation="MCKIVQRMLVLAIPAVAFSLAAAAPAKAQAQGSFERTLKVTGPV
                     DLEIRSGSGSIRVTPGSGDTVKISARIRSGRSWFSSSAADRIREIEKNPPIAQSGNSI
                     RVGGETDRSLFRNISISYDVTVPSRTRLDARTGSGSVDVGDLEGAVEAHSGSGSLDIG
                     RVAGPVVARTGSGSIDVRGAQSLEARAGSGSIKVTGIAGAVTAHSGSGAVRVEHVGRG
                     DLDVSSSSGHVTVTGVDGAARVSSSSGGVDVEGRPSGPWDIHSSSGSIVLRVPKDAAF
                     ELDARVSSGGIDLSHPVTLSGRVDRRRVQGQVRGGGPLVAVRSSSGGITIE"
     gene            complement(1766..3079)
                     /locus_tag="PKK95_11925"
     CDS             complement(1766..3079)
                     /locus_tag="PKK95_11925"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC18973.1"
                     /translation="MTPHQLPFAARALSHRNYRLFFTGQGLSLVGTWITRVATSWLVY
                     RLTGSAAALGIVGFVGQIPTFVLAPVAGVWVDRWGRYRVLVATQVLSMLQSFALAGLA
                     LTGVITVAHVVLLSVLQGIVNAFDTPARQAFVVDMVEDRADLPNAIALNSSMVNGARL
                     VGPSVAGVLVALAGEGWCFLIDGFSYVAVIVSLLMMRLPPAAPRAARRRVLAELNEGF
                     RYAFGFAPIRSILLLLALISLMGMPYTVLMPVMAADVLHGGPRTLGFLMGATGVGALA
                     GAIALASRRTVLGLGRVIAWAAVLFGAGLIAFSLSRNVALSMVLMVFTGAGFVVQLAS
                     SNTILQTIVREEMRGRVMAFYAMAFMGTAPFGSLLAGGLAARIGVPNTILLGGSACIA
                     GGAVFARQLPRLRQLIRPIYVERGILPEVAAGLSNTAVLRDETER"
     gene            complement(3205..4599)
                     /locus_tag="PKK95_11930"
     CDS             complement(3205..4599)
                     /locus_tag="PKK95_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18974.1"
                     /translation="MGKVNPLVVLFFLCVLFALLTFIVPGGAFDRRTIEVMGQTRSIV
                     VPGSFHYVPAVPQGFSELWTVFMRGALEGADRSFVILLSAGAMTAVIATGAIDAGIHL
                     LVRKTGRFGIAFIPIIVFALGLCGATFGMYEEAIPFILVIAPLMLTMGFDSMSAVFVM
                     YWSIAVGFACGITNPYTVAIGQALAGVPLYSGTGFRVVCFLVLMLVTSAVVIRYAVRV
                     KRDPSRSLSAGEDVENRKRLRASPDASTAAAMTPRRALALVVLLATFAILIVGLLRYE
                     WGFTEIGGLYFWMGIVVPLAGGLSVGEMIDKNIEGWRSVVIAVIMMSAAQILYFILHD
                     SLILDTILNYFASYLERVPQFVVAYVMFGISAATAALLGSASGTAATVMPILAPLADV
                     LRFSKQIVVLAFQMASGAFNFWMPWDGIAFTICSMAGVNFFRYIKASAKFAVLVYVPT
                     ALLLLFIAVEVGYR"
     gene            complement(4700..>5674)
                     /locus_tag="PKK95_11935"
     CDS             complement(4700..>5674)
                     /locus_tag="PKK95_11935"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOC18975.1"
                     /translation="GVSVANNMATMAGIPAERQFDVVPGGVIWTEFMQLVLEHATSAG
                     EGVALVGELCEERGLSCDTGTMLVVADPNEAWWVEMARDGRWAVQRVADDEVSVRANC
                     YRIPDFASRHGDPASQADRYNLDRHEALEARAACLSSVGPADLMSLLREVYEGTPLYR
                     TRPDGSPFRTGVRTVATMRTEAATVLDPDPGLPGGVAHRMWCCLSTPLTGVFVPFHAG
                     IRRIEPHYAASGGEFTEHSAYWRFTELAKLVDYRYPACANMVRATWNAFEAETFRDLA
                     AVESHCDGLDAPECATLLTDFDCRRASAAIRTLQALLREVKTRAYYEE"
BASE COUNT          957 a         1943 c         2013 g          761 t
ORIGIN      
        1 gggtcagcgc ggcggcagtg gctcgatgag gtcgcggtag gcgtgccagg tggcgtggcc
       61 gatgatcggc atcagcacca ccaggccgat gttgaaggtg gcgaagccga tggcggtgag
      121 gatgacgatc agcgccgccc acaccagcat cgggccgggg ttgttgagca gcgcgccgaa
      181 gctcgccagc atcgcggtga tcgcgtcctg gttgcggtcg agcatcagcg gcaccgagac
      241 gaccgtcgcg aggacgatcc acccgagggc gtaccagaag gtccggatct gccagcggaa
      301 gtgcgactcg aggaaggtgc cgcgcacccg gcttcgccgg acgtagttga tgatcacggc
      361 gacgatcgac ggcaggccga acaggaacga cgtcacgatg gccgccgagg tggccacgcc
      421 gagcacgacg ccgaacgcgt gcagggcgta gatgacgtgc gtccaggtga tgtcggacgg
      481 gtcggccacg accgccgccg tcctgggtgt ctggtccatt ctctgcgttc tccgttctgc
      541 cttcaggagt ccacgttctc cgtcttctgg tcttcgctcg ccccgtccct tcctacgtcg
      601 cttcctacgt cggttcgccc ggctgggttg cgcggaacca atcgcccgcg ccgtgcgttc
      661 tatccggtag cggcgcgtgc cggcccctgt cgctcagact gccggttggc agctctcctg
      721 gagacgcaca tgtgcaaaat cgtgcagcga atgctcgtcc tcgccatccc ggcggttgct
      781 ttcagcctgg cggctgccgc gccggccaaa gcccaggccc agggctcctt cgagcggact
      841 ctgaaggtga ccgggccggt cgacctggag attcggtcgg gctcgggcag catacgcgtc
      901 acccctggct ccggtgacac cgtgaagatc tccgctcgca tccggtccgg ccgctcctgg
      961 ttctccagca gcgccgccga ccgcatccgc gaaatcgaga agaacccccc gatcgcgcag
     1021 tcgggcaact ccatccgtgt cgggggcgag accgacagga gcctcttccg caacatcagc
     1081 atcagctacg acgtcacggt gccgtcccgg acgaggctcg atgcaaggac cgggtcggga
     1141 tcggtagatg ttggcgacct ggagggcgcc gtcgaggcgc acagcggctc cgggagcctc
     1201 gacatcggtc gcgtcgcggg accggtggtg gcgaggacgg ggtcgggcag catcgacgtg
     1261 cgcggggcgc agagcctcga ggcacgcgcc ggcagcgggt cgatcaaggt gaccggcatc
     1321 gccggggccg tgaccgctca cagcggatcg ggcgcggtgc gggtcgagca cgtcgggcga
     1381 ggcgacctcg acgtgtcgtc gtcttcgggg cacgtgacgg tgacgggcgt ggacggtgct
     1441 gcccgggttt cctcctccag cggcggcgtg gacgtggaag gacgcccgtc cggcccctgg
     1501 gacatccact catcctccgg gagcatcgtc ctgagggttc cgaaggatgc ggccttcgaa
     1561 ctcgacgccc gcgtcagctc gggcgggatt gacctgtcgc accctgttac gctgagcggg
     1621 cgcgtcgacc ggcgccgcgt gcaagggcag gttcgcggcg gcgggccgct ggtggcggtg
     1681 cgctcctcgt cgggcgggat caccatcgag tgacctcggg gtgcgtgtca cgcagggctg
     1741 aagccctgcg cgacgcctgt tctgctcacc gttcggtttc gtcgcgaagc accgccgtgt
     1801 tcgacaaccc ggccgccacc tcgggcagga tgcctcgctc gacgtagatg ggccggatca
     1861 gctggcgcag ccgcggcagc tgccgcgcga acacggcgcc acccgcgatg caggcggagc
     1921 cgccgagcag gatggtgttc ggcacgccga tgcgcgcggc gaggccgccg gcgagcaggc
     1981 tgccgaacgg cgcggttccc atgaacgcca tcgcgtagaa cgccatgacc cgcccccgca
     2041 tctcctcccg cacgatcgtc tgcaggatgg tgttgctcga ggcgagctgc accacgaagc
     2101 cggcgcccgt gaacaccatc aggaccatcg acagggcgac gttgcgagag agcgagaacg
     2161 cgatcaggcc ggcgccgaac aggacggcgg cccaggcgat cacccggcca aggcccagca
     2221 ccgtgcgccg cgaggcgagg gcgatcgcgc cggccagggc gccgactcct gtcgcgccca
     2281 tcaggaaccc gagcgttcgc gggccgccgt gcagcacgtc ggcggccatc accggcatca
     2341 gcacggtgta cggcatgccc atcaggctga tcagcgcgag cagcagcagg atcgagcgga
     2401 tgggtgcgaa tccaaaggcg tagcggaacc cctcgttcag ctcggcgagc acgcggcgcc
     2461 gggcggcgcg cggggcggcg ggcgggagcc gcatcatcag cagggagaca atcaccgcca
     2521 cgtacgagaa cccgtcgatc aggaagcacc agccctcgcc ggccagcgcc accagcacgc
     2581 cggcaaccga cggccccacc agccgcgcgc cgttcaccat cgacgagttc agcgcgatcg
     2641 cgttcggcag gtcggcgcgg tcctccacca tgtccacgac gaaggcctgc cgggcgggcg
     2701 tgtcgaaggc gttgacgatg ccctgcagca cgctcaacag cacgacgtgg gcgacggtga
     2761 tcacccccgt gagcgccagg ccggccaggg cgaacgactg cagcatcgac agcacctgcg
     2821 tggcgacgag cacccggtag cgcccccagc ggtccaccca gaccccggcg accggcgcga
     2881 gcacgaacgt ggggatctgt cctacgaagc cgacgatgcc gagggcggcg gccgaaccgg
     2941 tcaaccggta cacgagccag ctcgtggcaa cccgggtgat ccacgtgccg accagcgaca
     3001 ggccctggcc cgtgaagaac aggcggtagt tgcggtgcga gagggcgcgc gcggcgaagg
     3061 gcagctggtg cggagtcatg acgccccgcg cggcaggtgg ggacgcatcg tccgattgta
     3121 cggcagggcg cgcgtcgagc cgcggagcta ttcctgccgc cacacacgca tcgggtcgag
     3181 gcgggccgcg cggatggccg gcgcctaccg gtatcccacc tcgaccgcga tgaagagcag
     3241 cagcagcgcg gtcgggacgt agacgaggac cgcgaacttc gccgacgcct tgatgtagcg
     3301 gaagaagttg acgcccgcca tcgagcagat cgtgaaggcg atgccgtccc acggcatcca
     3361 gaagttgaac gccccgctcg ccatctggaa ggccagcacg acgatctgct tcgagaaacg
     3421 caggacgtcg gccagcggcg ccaggatcgg catcacggtg gccgcggtcc ccgacgcgct
     3481 ccccagcagc gccgcggtgg ccgcgctgat gccgaacatc acgtaggcga cgacgaactg
     3541 cggcacgcgc tccaggtagc tggcgaagta gttgaggatg gtgtcgagga tgagcgagtc
     3601 gtggaggatg aaatagagga tctgcgcggc gctcatcatg atcacggcga tcacgacgct
     3661 gcgccagccc tcgatgttct tgtcgatcat ctcgccgacc gagagcccgc cggccagcgg
     3721 gacgacgatc cccatccaga agtagagccc gccgatctcg gtgaagcccc actcgtagcg
     3781 cagcaggccg acgatgagga tggcgaaggt cgcgagcagc accaccagcg ccagcgcgcg
     3841 gcggggagtc atggccgccg ccgtcgaggc gtcgggcgag gcacgcagcc gcttccggtt
     3901 ctccacgtcc tccccggccg acagcgagcg ggacgggtcg cgcttgaccc gcaccgcgta
     3961 gcggatgacg acggcactcg tcaccagcat cagcacgagg aagcagacga cccggaagcc
     4021 cgtgcccgag tagagcggca cgccggcgag cgcctgcccg atggcgaccg tgtagggatt
     4081 ggtgatcccg caggcaaacc ccacggcgat gctccagtac atgacgaaca cggccgacat
     4141 cgagtcgaag cccatcgtca gcatcagcgg ggcaatcacc aggatgaagg gaatcgcctc
     4201 ctcgtacatc ccgaatgtcg cgccgcagag gccgagcgcg aacacgatga tcgggatgaa
     4261 cgcgatgccg aagcggccgg tcttgcgcac cagcaggtgg atgccggcgt cgatcgcgcc
     4321 ggtggcgatg acggccgtca tggctccggc cgagagcagg atcacgaacg agcggtcggc
     4381 cccctcgagg gccccgcgca tgaagacggt ccacagttcg gagaaaccct gcggaacggc
     4441 gggcacgtag tggaaggagc cgggcactac gatcgagcgg gtctgtccca tcacctcgat
     4501 ggtgcggcgg tcgaaagcgc cgcccgggac gatgaaggtc aggagcgcga agagcacgca
     4561 caggaagaag agaaccacca gggggttcac cttccccatc gccgccacga atcgctgtcg
     4621 ccacgccgcg cccacgcgcg gcgctctcgg ctccccgggc tcgtggggca acccttcgga
     4681 acggcccttt gcgctctcgc tattcctcgt agtaggcacg ggtcttcacc tcgcgcagga
     4741 gcgcctggag cgtgcggatc gcggccgacg cgcgccggca gtcgaagtcg gtgagcagcg
     4801 tggcgcactc gggggcgtcg agcccgtcgc agtgcgactc gacggccgcc aggtcgcgga
     4861 acgtctcggc ctcgaacgcg ttccaggtgg cgcgaaccat gttggcgcac gccgggtacc
     4921 ggtagtccac gagcttcgcc agctccgtga agcgccagta ggccgagtgc tccgtgaact
     4981 cgccgccgct ggccgcgtag tgcggctcga tccgccggat accggcgtgg aacgggacga
     5041 agactcccgt gagcggcgtc gagaggcagc accacatccg gtgggccacg ccaccgggca
     5101 ggcccgggtc gggatcgagg acggtcgcgg cctcggtccg catggtcgcc accgtgcgaa
     5161 ccccggtccg gaacggcgac ccgtccgggc gggtgcggta cagcggcgtc ccctcgtaga
     5221 cctcgcgcag cagcgacatg aggtcggcgg gcccgacgct cgacaggcag gcggcgcggg
     5281 cctcgagcgc ctcgtgccga tcgaggttgt agcgatcagc ctggctggcg gggtcgccgt
     5341 gcctcgaggc gaagtcgggg atgcgatagc agttggccct caccgagacc tcgtcgtctg
     5401 ccacccgctg caccgcccac cggccgtcgc gcgccatctc gacccaccac gcctcgttcg
     5461 ggtcggcgac gacgagcatg gtgccggtgt cgcagctcag gccgcgctcc tcgcacagct
     5521 cgccaacgag cgcgaccccc tcgcccgcag acgtcgcgtg ctcgaggacg agctgcatga
     5581 actcggtcca gatgacgccg ccgggcacga cgtcgaactg gcgctccgcg gggatcccgg
     5641 ccatggtcgc catgttgttg gccaccgaga cgcc
//
LOCUS       DAOPQX010000231         5666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15613_length_5666_cov_4.427731, whole genome shotgun sequence.
ACCESSION   DAOPQX010000231 DAOPQX010000000
VERSION     DAOPQX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15613_length_5666_cov_4.427731"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    399..498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(585..2528)
                     /locus_tag="PKK95_11940"
     CDS             complement(585..2528)
                     /locus_tag="PKK95_11940"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOC18976.1"
                     /translation="MSAEIRQDVVGDTSRAPVSQTPKLGKKATGEAAGYIGGQVSATI
                     DGHELKVPLGTTILEAAKTMGIRIPTLCHHPDLCVAGVCRVCVVEVEGQRTLQASCAY
                     PITAPIKVQTHTQKVRRARRHVIDLLLSEHCGECYACVRNNNCELQSLAKEFGVDFFR
                     FGHVEKPRFEIDSSSYSVIREPDKCVLCRRCVRTCIDVQEVGALEASGRSIGTRISTY
                     LDKPLGEVVCINCGQCINRCPTGALHAKDASDDVWRAIEDPSKHVVIQTAPSPRAGIG
                     ECFGLPPGVPMTFQMNTALRLAGFDKVFDTNFSADLTIIEEGTELLLRLYKALVAKEP
                     VAFPQFTSCSPGWVKFLEHFYPEYIPNLSSAKSPQQMFGAVIKTYYARKTGIDPKDIV
                     TVALMPCSAKKFECNRPEMGDSGFKDVDYGLTTRELAQMIKEAGIHLPDMPKSDFDDP
                     FGSRTGSGVIFGATGGVMEAALRTVLELVTGKKVENLFENANIVPVRGFDGVRYVELP
                     VTEVSPTVEPLIAHLVPNWDFLKGATLKLAVAHGTANAKKVLEDIKAGGPFSQCHFIE
                     FMACPGGCLGGGGQPIPTSPEIRAARAKAIYDEDAASEVRKSHENPAVVELYREFLTD
                     GPCGHVSHELLHTHYTPRGKYIG"
     gene            complement(2550..3755)
                     /locus_tag="PKK95_11945"
     CDS             complement(2550..3755)
                     /locus_tag="PKK95_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOC18977.1"
                     /translation="MTPVSSKKTPFTFDSIEPESGLKAALGKTRADIIAEISASNLRG
                     RGGAGFPTGTKWNLAAAAKVRPGGRKFVVCNADEGEPGTFKDRVILSEYADLVFEGMT
                     IAGLAIGAGDGILYLRGEYLYMRPHLEGVLARRRAANLLGKNVAGRDGFDFDIRIQMG
                     SGAYVCGEETALIESLEGNRGEPRNRPPFPVDTGFDGQPTVVNNVETLAWVACILARS
                     GQWFKSAGTAKSAGFKLFSVSGDCTAPGVYEFPLGVTVQELLDKVGGSGAKAVQIGGA
                     SGHCIPASEFSRRIAFEDIATGGSIIVFGPGRDMLEVAKNFMEFFVEESCGQCTPCRV
                     GNARLLEGIEMLERGECSMAYMRELLGLCETMQVASKCGLGQSSPNAFVSILNHFRDE
                     IMGRTAVAH"
     gene            complement(3774..4286)
                     /locus_tag="PKK95_11950"
     CDS             complement(3774..4286)
                     /locus_tag="PKK95_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOC18978.1"
                     /translation="MLVGQQQRLSDEIRGLVERYGRQRNALIPILQDLHASHASISDF
                     AMQVVADELGIHPSEVYGVVTFYQFLRRESQGRFVFRLCRTISCDLAGKARVARQLEN
                     DLGIGFGETSTDGRFSLEWASCLGMCDQGPALLVNEKVYTRVTPEQVHEIIEECKATF
                     GAHARQVAHA"
     gene            complement(4297..4683)
                     /locus_tag="PKK95_11955"
     CDS             complement(4297..4683)
                     /locus_tag="PKK95_11955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC18979.1"
                     /translation="MARILIVDDDPDIVFTTGLFLKKAQHEVLSASNREDGMKAIEQQ
                     NPDLIILDVMMEQPDDGIAMAQELRRQGNETPILMLTSVARVTGFAYDQDQELVPVDA
                     FHEKPIQPETLLATVEQLLVKRQQEC"
     gene            5131..5628
                     /locus_tag="PKK95_11960"
     CDS             5131..5628
                     /locus_tag="PKK95_11960"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC18980.1"
                     /translation="MPLKDALLPDLDRELGATRRLLERVPADRFDWRPHEKSRSLGQL
                     ATHLAAIPRWGVLVLQTTQSDIAGQPDGDDPAATPQEVLALFDRHAGTLRAQLAQTEG
                     DGPLLVRWTLEKDGQEVFSLPRVSALRTIAMNHLIHHRGQLTVYLRLLGVPLPPMYGP
                     TADEA"
BASE COUNT          825 a         1907 c         1910 g          924 t
ORIGIN      
        1 tgatctcgcg cgcggcgagc ttctgctcga gcatggcgcg gcaggcggca atccgttcca
       61 gcgccgcggg ggccagttcc acccgctcgc cgtggcgggc gacccgcacg agcttctcaa
      121 tggtcagcga cgagccgtcg aggaggatgg gcatgagcga ctccaggggc aggggcgaat
      181 catagcgtaa agtgtcgtgc agggggtgca gggggtgcag gaggtgcagg aggtgctggt
      241 gctggaggct tgcccgccgt agccccgccg aggagagcgt cggaccggag gcgggcggag
      301 gcgggtgcag gaggtgctgg tgctggaggc ttgcccgccg tagcccgccg aggagagcgt
      361 cggaccggag gcgggcggag gcgggtgcag gaggtgctnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnngg tgctggaggc ttgcccgccg tagccccgcc gaggggagcg
      541 tcggaccgga ggcggggcgg ccacgagggc cgccccgctg tccgctaccc gatgtacttc
      601 ccgcgcggcg tgtagtgcgt gtgcagcagc tcgtggctga cgtgcccgca cggaccgtcg
      661 gtgaggaact cgcggtacag ctccacgacc gccgggttct cgtgcgactt gcgcacctcg
      721 gacgccgcgt cctcgtcgta gatcgccttg gcccgcgccg cgcgaatctc gggcgaggtg
      781 gggatcggct ggccgccgcc gcccaggcac ccgcccgggc acgccatgaa ctcgatgaag
      841 tggcactggg agaacggccc gccggccttg atgtcctcga gcaccttctt ggcgttggcc
      901 gtgccgtgcg cgaccgccag cttgagcgtc gcccccttga ggaagtccca gttcgggacg
      961 aggtgggcga tcagcggctc cacggtgggg ctcacctcgg tcacgggcag ctcgacgtag
     1021 cgcaccccgt cgaacccgcg caccgggacg atgttggcgt tctcgaacag gttctcgacc
     1081 ttcttgccgg tcaccagctc gagcacggtc cggagggcgg cctccatgac gccgccggtg
     1141 gcgccgaaga tcacgcccga tcccgtgcgg ctgccgaacg ggtcgtcgaa gtcgctcttc
     1201 ggcatgtcgg gcaggtggat gcccgcctcc ttgatcatct gcgccagctc gcgcgtggtc
     1261 aggccgtagt cgacgtcctt gaacccgctg tcgcccatct cgggccggtt gcactcgaac
     1321 ttcttggccg agcagggcat cagcgcgacg gtgacgatgt ccttcgggtc gatcccggtc
     1381 ttccgggcgt agtaggtctt gatcaccgcc ccgaacatct gctgcgggct cttggccgac
     1441 gacaggttcg ggatgtactc cgggtagaag tgctcgagga acttgaccca cccgggcgag
     1501 cagctggtga actgcgggaa ggcgaccggc tccttcgcca cgagcgcctt gtagaggcgg
     1561 agcagcagct cggtgccctc ctcgatgatc gtgaggtcgg cgctgaagtt ggtgtcgaag
     1621 accttgtcga acccggccag gcgcagcgcc gtgttcatct ggaaggtcat cggcacgccc
     1681 ggcggcaggc cgaagcactc gccgatcccg gcccgcggac tgggcgcggt ctggatgacc
     1741 acgtgcttgg acggatcctc gatcgcgcgc cacacgtcgt cagacgcgtc cttggcgtgc
     1801 agggcgccgg tcgggcagcg gttgatgcac tggccgcagt tgatgcagac cacctcgccg
     1861 agcggcttgt cgaggtaggt cgagatccgg gtcccgatgc tgcgccccga cgcctcgagc
     1921 gccccgacct cctgcacgtc gatgcaggtg cggacgcagc ggcggcagag gacgcacttg
     1981 tccggctcgc ggatcaccga gtagctggac gaatcgatct cgaagcgggg cttctccacg
     2041 tggccgaagc ggaagaagtc gacgccgaac tccttggcca gcgactgcag ctcgcagttg
     2101 ttgttccgca cgcaggcgta gcactcgccg cagtgctcgg agagcagcag atcgatcacg
     2161 tggcggcgcg cccgccgcac cttctgggtg tgggtctgga ccttgattgg cgccgtgatc
     2221 gggtaggcgc aggacgcctg cagcgtccgc tgcccctcca cctcgacgac gcagacgcgg
     2281 cagacgccgg caacgcacag gtcggggtgg tggcagaggg tggggatgcg gatccccatg
     2341 gtcttggccg cttcgaggat cgtcgtcccc aggggcacct tcaactcgtg gccgtcgatg
     2401 gtcgccgaca cctggccgcc gatgtacccg gccgcctccc cggtggcctt cttcccgagc
     2461 ttcggggtct ggctcaccgg cgcccgcgac gtgtcgccca cgacatcctg tctgatttca
     2521 gcgctcatcg ttcacctctg acccggcgtt cagtgggcca ccgccgtgcg tcccatgatc
     2581 tcgtccctga agtggttcag gatcgacacg aaggcgttcg ggctcgactg gcccaggccg
     2641 cacttcgagg ccacctgcat cgtctcgcag aggcccagca gttcgcgcat gtaggccatc
     2701 gagcactcgc cgcgctcgag catctcgatc ccctcgagca gccgcgcgtt ccccacccgg
     2761 cagggggtgc actgcccgca cgactcctcg acgaagaact ccatgaagtt cttcgccacc
     2821 tcgagcatgt cgcggccggg gccgaacacg atgatcgacc cgccggtggc gatgtcctcg
     2881 aaggcgatcc ggcgcgagaa ctccgaggcc gggatgcagt ggcccgacgc gccgccgatc
     2941 tgcaccgcct tggcgcccga gccgcccacc ttgtcgagca gctcctgcac cgtcacgccg
     3001 agcgggaact cgtacacgcc cggggccgtg cagtcgcccg agaccgagaa gagcttgaag
     3061 ccggccgact tggcggtgcc ggcgctcttg aaccactggc cgctgcgcgc caggatgcag
     3121 gccacccagg cgagcgtctc gacgttgttg accacggtgg gctggccgtc gaagccggtg
     3181 tccaccggga agggcggccg gttgcgcggc tcgccgcggt tgccctcgag cgactcgatg
     3241 agcgccgtct cctcgccgca gacgtaggcg ccgctgccca tctggatccg gatgtcgaag
     3301 tcgaagccgt cgcggcccgc cacgttcttc ccgagcaggt tggccgcccg gcgccgggcc
     3361 agcaccccct cgaggtgcgg gcgcatgtag aggtactcgc cgcgaagata aaggatgccg
     3421 tcaccggcgc cgatggcgag acccgcgatg gtcatgccct cgaacaccag gtcggcgtat
     3481 tcggacagga tcacccggtc cttgaaggtg ccgggctcgc cctcgtcggc gttgcagacg
     3541 acgaacttgc ggccgccggg cctgaccttc gcggccgccg cgaggttcca cttggtcccg
     3601 gtcgggaagc cggcgccgcc gcgcccccgc aggttcgacg cgctgatctc ggcgatgatg
     3661 tcggctcggg tcttcccgag ggcggccttg aggccggact ccggctcgat gctgtcgaag
     3721 gtgaacggtg tcttcttgct gctgacggga gtcatcgcaa cctgcccttc gtcctaggcg
     3781 tgggccacct ggcgcgcgtg ggcgccgaag gtcgccttgc actcctcgat gatctcgtgc
     3841 acctgctcgg gggtcacccg ggtgtagacc ttctcgttga ccagcagcgc cggcccctgg
     3901 tcgcacatgc cgaggcagct cgcccactcg aggctgaagc ggccgtcggt gctcgtctcg
     3961 ccgaacccga tgccgaggtc gttctcgagc tgccgggcga cgcgcgcctt gcccgccagg
     4021 tcgcacgaga tcgtccggca gaggcggaag acgaagcgcc cctgcgattc gcgccgcagg
     4081 aactggtaga aggtgaccac gccgtagacc tccgaggggt ggatgcccag ctcgtcggcc
     4141 acgacctgca tggcgaagtc cgagatgctc gcgtgcgacg cgtgcaggtc ctgaaggatg
     4201 ggaataaggg cgttacgctg ccgcccatac cgttcgacca ggccgcggat ctcgtccgac
     4261 agccgctgct gctgccctac tagcatcgtc ccctcctcag cactcctgct gccgcttgac
     4321 cagcagctgc tcgaccgtcg ccagcaaggt ctcgggctgg atcggcttct cgtggaaggc
     4381 atcgaccggc accagctcct ggtcctggtc gtaggcgaac ccggtcaccc gcgcgacgct
     4441 cgtcagcatc aggatcggcg tctcgttccc ctgccggcga agctcctgcg ccatcgcgat
     4501 cccgtcgtcc ggctgctcca tcatcacgtc gaggatgatg aggtcggggt tctgctgctc
     4561 gatcgccttc atcccgtcct cccggttgga ggcggacagg acctcgtgct gcgccttctt
     4621 gaggaacagc ccggtggtga acacgatgtc cgggtcgtcg tccacgatga ggattctggc
     4681 catgtcgcgg tccctctttc tgctcgaaga agcgtccggg cgcgagaacg gctcccgccg
     4741 gacgcggcgc gggccggatc gtgctccggc ggcccatcac tgcgctgagg tcacaacccg
     4801 ggcgcgcaag cccggcggaa agaccacccg aggcgatcgg cgctgcgcgg gcgccgacgt
     4861 ggaggctgct ggcgggcttg tgaagggcgg cacaagcaat tgtgcgccgt ttcacaactg
     4921 gctattctat ccgttgaggc tccccttccg caacggggtc gggagtaatt agaagtcatg
     4981 ccggtcctct gacttctatc cgcaacgggg tcgggagtaa ttagaagtca tgccggtcct
     5041 ctgacttctc cgcaacgggg tcgggagtaa ttagaagtca tgccggtcct ctgacttcta
     5101 attactcccg accccttgga gagactgagt atgccgctca aggacgccct gctgcccgac
     5161 ctcgaccgcg aacttggcgc cacccggcgc ctgctcgagc gggtgccggc ggaccggttc
     5221 gactggcgcc ctcacgaaaa gtcgagatcc ctcgggcagc tggccacgca cctggccgcg
     5281 atccctcgct ggggcgtact ggtgctacag accacccagt ccgacatcgc cggccaaccc
     5341 gatggcgacg atccggcggc caccccgcag gaggttctcg cgctgttcga ccgccacgcc
     5401 ggcacgctgc gcgcgcagct ggcgcagacg gagggcgacg gccccctgct cgtgcggtgg
     5461 acgctcgaga aggacggcca ggaggtcttc agcctgcccc gggtcagtgc gctgcggacc
     5521 atcgccatga atcacctgat ccaccaccgc ggccagctca cggtctacct gcggctgctc
     5581 ggcgtcccgc tgccgccgat gtacggcccc acagcggacg aagcctgaag ctattccgtt
     5641 tgccagtgtc cgctcgcgag cgccac
//
LOCUS       DAOPQX010000232         5666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15614_length_5666_cov_4.294065, whole genome shotgun sequence.
ACCESSION   DAOPQX010000232 DAOPQX010000000
VERSION     DAOPQX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15614_length_5666_cov_4.294065"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..905)
                     /gene="kdsB"
                     /locus_tag="PKK95_11965"
     CDS             complement(117..905)
                     /gene="kdsB"
                     /locus_tag="PKK95_11965"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOC18981.1"
                     /translation="MHPGRDSAGFPILSPACRLVVIPARYQSSRLPGKPLEPIAGRPM
                     IQHVYERATRAKLVDAVVVATDDDRIRQAVERFGGRVWVTRTDHETGSDRVAEVVAGL
                     DCDLVINVQGDEPLIEPTAIDQAIEPLLAEPALQMTTLCRRIDDPSLLASRDVVKVVR
                     DLRGNALYFSRAPIPFARREGAGPLGWKHIGLYAYRRDFLLRFARLPAGPLERLESLE
                     QLRALEHGLDIRVVETTYDSVGVDTPADLERVRRLAAALPQAEH"
     gene            967..2235
                     /gene="brnQ"
                     /locus_tag="PKK95_11970"
     CDS             967..2235
                     /gene="brnQ"
                     /locus_tag="PKK95_11970"
                     /inference="COORDINATES: protein motif:HMM:NF017347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HOC18982.1"
                     /translation="MRKTSTDVMVVGVALFAMFFGAGNLIFPPFIGMAAGTAWTAALL
                     GFLATGIGMPLLGVVASARAGGSVEHLAGRVGPRFARILSVVIILAIGPLLAIPRTGA
                     TTFELGVVPLAPWFSPALFSIVYFGVTLYFAINKSAVVDRIGKVLTPFLLVTLAWIVV
                     KGVLTPLGPLVDQGLDRPFATGFREGYQTMDAMASVVFAEIVIGALVFKGYRSVTDQV
                     RLASLAGVIAAVGLGLVYGGLMYVGATGATRFPEGIERTQLVVAISQALLGETGKVAL
                     GLAVALACLTTSIGLTATVADYFSGLSRGKIGYKAICVATVVFSGVFATVGVTTIVRV
                     AVPLLVTVYPVVIVLILLTIGGVSHRGVYTGAVTGALAVGLLDAFTAAGTPIAAANAL
                     VAHIPFAAAGFPWILPALAGGALGLLLPKK"
     gene            2251..2790
                     /locus_tag="PKK95_11975"
     CDS             2251..2790
                     /locus_tag="PKK95_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18983.1"
                     /translation="MTAGRALGLATILALAATWLASSYLANRPTPLPLDEPLPISQTE
                     VGRATARLALGLKSLAAPSRHAAYKAPVRNPFRFGATSGPPNQPGPSARRTSSETLAR
                     GASDLPPERPSMRLSGMAEDPGPRGPVRRAVIVAADQLFVVGTGDLVLARFRVTRIDA
                     EAVELEDLLGGPRTVLALR"
     gene            complement(2841..3686)
                     /locus_tag="PKK95_11980"
     CDS             complement(2841..3686)
                     /locus_tag="PKK95_11980"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOC18984.1"
                     /translation="MADTTTLTEVVFRIRRFDPTSDAAPRWEDYRLPYTKGMTVLDGL
                     KAVKESQSPTLSWRSSCRMGVCGSCGMFINGRPRLACNTQITELGTSLVSVAPLPNFK
                     TVRDLVPDLTPMFDAHAALHPYIVRADQAEQERPTGEYWQSVHQLEAYLQFSYCIKCG
                     CCMAACPTYATDPVYSGPMPLAQAYRYTADSRDGGFAARKAALAAERGPWKCHFAGEC
                     SQVCPKGVDPARAIQLMKRALVLDYLRLWKQPCPAKLATVPAGIKRREGIPEAPAPTI
                     EKMVT"
     gene            complement(3717..4094)
                     /locus_tag="PKK95_11985"
     CDS             complement(3717..4094)
                     /locus_tag="PKK95_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18985.1"
                     /translation="MRDQRLWTWHVGAGAVILVLLGLHMTVMHLDAFVRVFNATAEEP
                     IAWSNVFARAQSTFFLVSYVLLLGAALFHGFYGLRTIVFELNPGDGLKKAVNGALVAA
                     GLALFLFGTWAAWASFEFARVNG"
     gene            complement(4104..4553)
                     /locus_tag="PKK95_11990"
     CDS             complement(4104..4553)
                     /locus_tag="PKK95_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18986.1"
                     /translation="MIESKGRPNRLGIVGWLGGGRWGLERYLYTLHRVTGLGLLLYFV
                     LHIVVTSSRAVGADLEARKAWWEWAMGNVSGGMFVAGEYLVFAAFAFHALNGIRLGLV
                     ELGIGIGRPIEPVYPYRTSVHEQRPLAIAVMVLAAIVALAGGYGFLH"
     gene            complement(4574..>5666)
                     /locus_tag="PKK95_11995"
     CDS             complement(4574..>5666)
                     /locus_tag="PKK95_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOC18987.1"
                     /translation="YSETVTGDGMAMAYRAGLPLEDMEFLQFHPTGLVPSGILMTEAC
                     RGEGGYLKNAAGERFMQRYAPGKMELAPRDMVSRAEQTELLEGRGVTGPDGLPCLHLD
                     LTHLGAERINSRLPLIREVCMKFIGLDPIEKPIPIRPVAHYSMGGIETDINGLTRVPG
                     IWAAGEAAAVSLHGANRLGSNSTGECLVWGGITGAGIARALPGLPPPAQPSDGLLAAR
                     RGHIRELLERQGDENLYALRRELRATMDRDAGVFRTREALCNGLSVVREIRERASRAP
                     VPDKGSLYNSNLFHALELENLLDLAEVTLVGAIAREESRGAHARRDFAARDDERWLRH
                     TLAWHTGGAPRLEYKPVTIDTWKPVERKY"
BASE COUNT          802 a         2014 c         1962 g          888 t
ORIGIN      
        1 gccgtgccct tcgagcagcg ccccgatcga ggccgccgcc agccccttgc cgagcgacga
       61 caccacgccg cctgtgacga agatgtactt gggtgagcgc ccgtcggtct gctgcatcag
      121 tgctccgcct ggggcaaggc cgccgcgagg cgccggaccc gctccagatc tgccggggtg
      181 tccaccccga ccgaatcgta cgtagtctcc acgaccctga tgtccaggcc gtgctccagc
      241 gcgcgcagct gctcgagcga ctcgagccgc tcgagcggcc cggcgggcag ccgcgcgaac
      301 ctgaggagga agtcgcgacg gtaagcgtag aggccgatgt gcttccagcc gagcgggccg
      361 gctccctcgc gccgcgcgaa cgggatcggc gcccgcgaga agtacagggc gtttccccgc
      421 aggtcgcgca cgaccttgac gacgtcgcgg ctcgcgagca gcgacgggtc gtcgatccgc
      481 cggcagagcg tcgtcatctg gagcgccggc tccgcgagga gcggctcgat cgcctggtcg
      541 atggccgttg gctcgatcag cggctcgtcc ccctggacgt tgatcaccag gtcgcagtcc
      601 aggccggcga ccacctctgc cacccggtcg ctccccgtct cgtggtccgt ccgcgtcacc
      661 cacacccggc cgccgaaccg ctccacggcc tgccggatac ggtcgtcgtc ggtcgccacg
      721 accaccgcgt ccaccagctt cgcccgggtc gcccgctcgt agacgtgctg gatcatcggg
      781 cggcccgcta tcggctccag cggcttgccg ggcaggcggc tcgactggta cctcgcgggg
      841 atgacgacga gtcggcaggc gggggagaga atcgggaagc cagcggaatc gcggccaggg
      901 tgcacggcga atcatagcac accgctgtag gcaaaacggc tggagcggtg tacccttctg
      961 cgcttgatgc ggaagacctc aaccgacgtg atggtcgtgg gcgtcgcgct tttcgcgatg
     1021 ttcttcggcg cgggcaacct catcttcccg ccgttcatcg ggatggcggc cggaaccgcg
     1081 tggacggcgg ctttgctcgg cttcctcgct accggcatcg gcatgccgct gctcggcgtc
     1141 gtcgcgtcgg cccgggcggg cggcagcgtc gagcacctgg ccggccgtgt cggcccacgc
     1201 ttcgcccgca tcctgagcgt cgtcatcatc ctcgccatcg gtccgttgct ggcgatcccg
     1261 cgcaccggcg ccaccacctt cgagctgggc gtcgtgccgc tcgcgccgtg gttcagcccg
     1321 gcgctgttct cgatcgtcta cttcggcgtc acgctctact tcgccatcaa caagtcggcg
     1381 gtcgtggacc ggatcggcaa ggtcctcacg ccgttcctgc tcgtcacgct tgcttggatc
     1441 gtcgtcaagg gcgtcctcac cccgctcggg ccgctagtgg accagggcct cgaccgaccg
     1501 ttcgcgacag gcttccgcga gggctaccag acgatggacg cgatggcgtc ggtggtcttc
     1561 gccgagatcg tgatcggggc gctggtgttc aaggggtacc gctccgtgac cgaccaggtc
     1621 aggctcgcct cgctcgccgg ggtgatcgcg gccgtcgggc tcggcctggt ctacgggggc
     1681 ctgatgtatg tcggcgccac cggcgcgacc cggttcccgg aaggcatcga acggacgcag
     1741 ctggtcgtgg cgatttcgca ggcgctgctc ggcgagaccg gcaaggtggc gctcgggctg
     1801 gcggtcgccc tggcgtgcct cacgacctcg atcggcctga ccgcgacggt ggccgactac
     1861 ttcagcggcc tgtcgcgcgg caagatcggc tacaaggcga tctgcgtggc gacggtcgtc
     1921 ttcagcggcg tgttcgccac cgtgggcgtg acgacgatcg ttcgcgtggc cgtgccgctg
     1981 ctggtcaccg tgtacccggt cgtcatcgtc ctcattcttc tgacgatcgg cggcgtgtcc
     2041 caccgaggcg tctacacggg cgccgtgacc ggcgcgctcg ccgtgggcct gctcgacgcc
     2101 ttcacggccg cgggcacgcc gatcgccgcc gccaacgctc tggtcgccca tatcccgttt
     2161 gcggcggccg gcttcccctg gatactcccg gcgctcgccg gcggcgcact tgggttgctg
     2221 ctgccgaaga agtaggaatc aggagtcggg atgactgcag gcagggcgct tgggctggcg
     2281 accatccttg cgttggccgc gacgtggctc gcgtcgagct acctggcgaa ccggcccacg
     2341 ccgcttccgc tcgacgagcc gctgccgatc agccaaaccg aggtcggccg ggcgaccgcg
     2401 cgacttgccc tcggcctcaa gtcgctggcc gcaccctccc gccacgccgc ctacaaggct
     2461 ccggtacgca acccgttccg cttcggtgcc acgagcggtc cgcccaacca gcccggccca
     2521 tcagctcgaa ggacttcctc ggagacactg gcgcgcgggg cctccgacct gccgccggaa
     2581 cgcccgtcca tgcgcctgag cgggatggcc gaagaccccg ggccgcgggg cccggtgcgc
     2641 cgcgccgtga tcgtcgccgc ggatcagctc ttcgtggttg gaactggaga ccttgtgctg
     2701 gcacggttcc gggtgacgcg catcgacgcc gaggccgtcg agctggagga ccttctgggc
     2761 ggaccgcgaa ccgtcctggc tttacggtga tcgcgactcg cgactcccga atcccgattc
     2821 aatcccggat ttctgattcc ctacgtgacc atcttctcga tcgtcggcgc cggcgcttcg
     2881 gggattccct cacggcgctt gatgccggcc ggcacggtgg ccagcttcgc cgggcagggc
     2941 tgcttccaca ggcgaaggta gtcgaggacg agggcccgct tcatcagctg gatggcccgc
     3001 gccgggtcta ctcccttcgg gcacacctgg gagcactccc cggcgaagtg gcacttccac
     3061 gggccacgtt cggcggcgag cgccgccttg cgcgccgcga accctccatc ccggctgtcg
     3121 gccgtgtagc ggtaagcctg agcgagcggc atcggccccg agtacacggg gtcggtcgcg
     3181 taggtggggc aggccgccat gcagcagccg cacttgatgc agtaggagaa ctggaggtag
     3241 gcctccagct ggtggaccga ctgccagtac tcgcccgtcg gccgctcctg ctccgcctgg
     3301 tcggcgcgaa cgatgtacgg gtggagggcc gcgtgcgcgt cgaacatcgg ggtcaggtcg
     3361 ggaaccaggt cgcgcaccgt cttgaagttg ggcagcgggg ccacggagac cagcgaggtg
     3421 cccagctcgg tgatctgggt gttgcaggcc aggcgcggcc gcccattgat gaacatcccg
     3481 cacgatccgc acacgcccat ccggcacgac gaccgccacg acaaggtcgg cgactgcgat
     3541 tccttgacgg ccttcaggcc gtcgaggacg gtcatcccct tcgtgtacgg caggcgatag
     3601 tcttcccagc gcggggccgc gtcgctcgtc gggtcgaagc ggcggatgcg gaagacgacc
     3661 tcggtcagcg tggtggtgtc agccatcgaa gttcccccgt cctgcgggca gccctgctac
     3721 ccgttgaccc gggcgaactc gaagctcgcc cacgcggccc acgtgccgaa caggaacaac
     3781 gccaggccgg cggccaccag ggccccgttc acggccttct tgagcccgtc tcccgggttc
     3841 agctcgaaca cgatggtgcg cagcccgtag aacccgtgga agagcgccgc gccgagcagc
     3901 agcacgtagg acaccaggaa gaaggtgctt tgggcgcggg cgaagacgtt cgaccaggcg
     3961 atcggctcct cggcggtcgc gttgaacacc ctgacgaagg cgtcgaggtg catcactgtc
     4021 atgtgcagcc ccaggagcac gaggatgacg gcacccgcgc cgacgtgcca cgtccacaag
     4081 cgttgatcgc gcacgtgatc ctcctagtgc aggaatccgt agccgccggc gagggcgacg
     4141 atggcggcga gaaccatgac cgcgatggcc agcggccgct gctcgtgcac cgaggtgcgg
     4201 taggggtaga ccggctcgat gggccggccg atcccgatgc ccagctcgac gagccccagc
     4261 cggatcccgt tcagggcgtg aaaggcgaat gcggcgaaca cgaggtactc gcccgcgacg
     4321 aacattccac cgctgacgtt ccccatggcc cactcccacc acgccttgcg ggcctcgagg
     4381 tcggcgccga cggcgcgcga cgaggtgacg acgatgtgca ggacgaagta cagcagcagg
     4441 ccgaggccgg tcacccggtg cagcgtgtag agatagcgct cgaggcccca gcggccccct
     4501 cccagccatc ccacgatccc gaggcgattg ggccgtccct tgctctcgat catggtgatc
     4561 ctctggagga ctctcagtac ttccgttcca ccggcttcca ggtgtcgatc gtcaccggct
     4621 tgtactcgag gcgcggcgcc ccgccggtgt gccaggcgag cgtgtggcgc agccatcgct
     4681 cgtcgtcgcg cgcggcgaag tcgcgccgcg cgtgcgcgcc ccgcgactcc tcgcgcgcga
     4741 ttgcgccgac gagggtgacc tccgcgaggt cgagcaggtt ctcgagctcg agggcgtgga
     4801 acaggttgga gttgtagagc gaccccttgt cggggaccgg ggcgcgcgag gcgcgctcgc
     4861 ggatctcgcg gacgaccgac agcccgttgc acagcgcctc gcgggtgcgg aagacgcccg
     4921 cgtcccggtc catcgtcgcc cgcagctcgc ggcggagcgc gtagaggttc tcgtcgccct
     4981 gccgctcgag cagctcgcgg atgtgccccc gccgggcggc caggagcccg tcggacggct
     5041 gcgccggggg cggcaggccc ggcagggcgc gcgcgatccc ggccccggtg atcccgcccc
     5101 acacgaggca ctcgccggtg gagttcgagc cgagccggtt ggcgccgtgc agcgagaccg
     5161 cggccgcctc gcccgccgcc cagatgcccg gcacgcgggt caggccgttg atgtcggtct
     5221 cgatgccccc catcgagtag tgggcgacgg gccggatcgg gatcggcttc tcgatcgggt
     5281 cgaggccgat gaacttcatg cagacctcgc ggatcagcgg caggcgcgaa ttgatccgct
     5341 cggccccgag gtgcgtgaga tcgaggtgca ggcaggggag gccgtccgga ccggtgacgc
     5401 cccgtccctc gagcagctcg gtctgttcgg cgcgcgacac catgtcgcgc ggcgccagct
     5461 ccatcttgcc cggcgcgtag cgctgcatga accgctcgcc ggcggcgttc ttgaggtatc
     5521 cgccctcgcc gcggcacgcc tcggtcatca aaatcccgct cggcaccagg cccgtcgggt
     5581 ggaactggag gaactccatg tcctcgagcg gcaggcccgc gcggtaggcc atcgccatcc
     5641 cgtcgccggt caccgtctct gagtac
//
LOCUS       DAOPQX010000233         5622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15787_length_5622_cov_4.919885, whole genome shotgun sequence.
ACCESSION   DAOPQX010000233 DAOPQX010000000
VERSION     DAOPQX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15787_length_5622_cov_4.919885"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..1183
                     /locus_tag="PKK95_12000"
     CDS             407..1183
                     /locus_tag="PKK95_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18988.1"
                     /translation="MPAPPAAKSPGPDPAADEAARLLTQNARVEAARARGRAESAGAE
                     RLAGETYAAAVDAERAAAEQFRSGAPERARLAFIEVVALFERAESEARAEQARQRELE
                     RRAAEPPVTSSPPATAVPTIPSSAGAAGAGGAAPPEPPPGAADAVAAREAIRSLLSRY
                     TAALESRSLARLKQVWPGLSGAQERAIADEFQHARSIAVTLADEQIDVRGPTATVSCR
                     RAYRLETRDGQRLESVTRAEFGLRQAGGGWVIEHVRHEQR"
     gene            1229..2539
                     /locus_tag="PKK95_12005"
     CDS             1229..2539
                     /locus_tag="PKK95_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18989.1"
                     /translation="MKRPLIAIALLVSLAAPAAGQSRTDQLALLFQEVFGPNGLVVNS
                     ESVLPDGSTHSAHFNSGFQSNFSQFNIAIAGQLASLPLPSPASGFTYTFDTTTGTFVR
                     STQSYGPILTDRAETIGRGRFSFGFNVQFFSFDTLEGLNLGGIPAVFTHDNFQLGGGL
                     ADIVSTVNSIEASVGQMTAAVTYGVTGRFDVSLAVPMVRTRLSVRSDASIQRVGTEDP
                     SIHFFRDPDAPEGYGTHRQFFASGTATGLGDVLVRVKGTAFREGSRGVALGLDVRLPT
                     GDERDLLGSGALGIKPFAAVSLGWGRFSPHINVGYQWNGRSVLAGNVKADTRAKMPDQ
                     LLFSVGADIGVSDRFSLAADVLGARAIDSPRLFPTSFTPQGSSQAFPDVAFRTSTFTM
                     TAGAVGFKIALARRLLANFNLRFRLSDAGLVDRASPLFGVEYGF"
     gene            2608..3771
                     /gene="iadA"
                     /locus_tag="PKK95_12010"
     CDS             2608..3771
                     /gene="iadA"
                     /locus_tag="PKK95_12010"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HOC18990.1"
                     /translation="MLLLKNAEVFDPEPRGRADILVAGGRIERVAPGLSLPADLCDVV
                     DAGPSFVVPGFIDCHVHVLGGGGEGGYATRTPELRLSDAVEGGVTTVVGCLGTDGVTR
                     SLPALLAKARGLEEEGLTTFIYSGHYAVPLQAMTGSIERDLLLVDKVIGAGEVAVSDH
                     RSTQPTFDELARLAAEARRGGILSGKAGVVNVHLGDGPRGLALLRRLVEETEIPASQF
                     LPTHMNRNPRLFEEAVAWVAGGGLADFTTSTVPAFVEEGEVKAALALRRMMEAGAPVA
                     NLTFSSDGQGSLPRFDGEGRLVGLDVGRVTSLFASVREAVLEHGVALPTALALITSNP
                     ARILKLRGKGRVAEGADADLVLLDPHALAVTGVIARGRWLMRDGEPLVRGTFE"
     gene            3801..5186
                     /locus_tag="PKK95_12015"
     CDS             3801..5186
                     /locus_tag="PKK95_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOC18991.1"
                     /translation="MPHTLVVVMSLVVLVLALSWMVPSGEYQRVQAETSAGPRTVTAP
                     GTYQQVEKIYLGPQMVLEAPLKGFVDGALLIAFLLMIGGSFAIFQETGAVDFGIRRLT
                     GALTARPHLEGLLIPALMIIFSLAGSVFGMSEEVIPFILIFIPLARRLGYDSIVGVSI
                     PFLGAAAGFAAAFFNPFTVGIAQSIAGLPLYSGLGYRLFTWVVGTAVMVAYVMWYAGR
                     VRRDPDISPVRDIDLRREAAGSHAGPREGWDARHVATLALFVASMVLLVVGVLAWKWY
                     IDQIAVLFFGMGIALGFAGGMGPSRVARTFVAGAKDMVGVVFIIACARALLVIAQDAK
                     ILDTILYWASNTLSALPRGVIAQVMFLIQCVINFFIHSGTAQAALTMPIMAPLADLVG
                     ITRQTAVYAYQLCEFINPILPTSAVTMGVLGAAGIPWERWARWFLPLMLILVVLSFLL
                     LIPPVLWFEWR"
     gene            5191..>5622
                     /locus_tag="PKK95_12020"
     CDS             5191..>5622
                     /locus_tag="PKK95_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC18992.1"
                     /translation="MRRALPIVAVVLVALAAGGQGFSPVPAGQGPNPAQDFVWPGERE
                     KLPAPAADAVASLGADRLATHVAFLSAPALGGRGLGDEGLEAAIEYVAAQLSLAGVAP
                     ALTASGAQPLEAYYQPVTLRAVSRPGGRLTISRGSGAGAVQT"
BASE COUNT          723 a         1960 c         2067 g          872 t
ORIGIN      
        1 ccccgtgagc tccgtgccag cggccgcgtc gcgcccgcca gcgagatggc acgccggacc
       61 gggtgcctgg gttgccgcgg cggtcgtcgt ggcgggaatc gccaccacgg tggcgtggct
      121 gacgtggttt cagccccaag gatccgcgcc ggcgggtggg accaccgtcc acggcgtgcc
      181 cacgtcggga acagcgggtc aagccacggg agccggtcct gctgtcgagg gaccgcgcga
      241 caaagtctcc gaggcggcgg ccgtcccggg cggagcacca cccagcccgg cggccgcggc
      301 gccagccgcc cctccaccgc catccgcgcc tgcgacggcg gagcgccgtc gcgacgaggg
      361 ggaagaggcg cggcggccgc agcggcgggg ggagcccggt gcgaacgtgc ccgcgcctcc
      421 ggcggcgaag agccccggtc ctgacccggc ggccgatgag gcggcgcggc tgctgactca
      481 gaacgcgcgg gtcgaggcgg cccgggcgag gggacgggcc gagtcggcgg gggccgagcg
      541 cctggccggc gaaacctacg ccgcggcagt cgatgccgag cgggctgccg cggagcagtt
      601 ccgatcgggc gcgccggagc gggcacggct ggcgttcatc gaggtggtgg ccctcttcga
      661 gcgcgccgaa agcgaggcgc gggcggaaca ggcgaggcag cgggagttgg agcggcgggc
      721 cgccgaaccg cccgtcacct cgagcccgcc ggcgacggcg gtgcccacca tcccaagctc
      781 ggccggggcg gctggcgcag gcggtgcggc gccccccgag ccgccgcccg gcgctgccga
      841 cgccgttgcg gcacgggaag cgatccggtc gcttctctcg cggtacaccg ccgcgctcga
      901 gtcgcgctcg ctggcgcgtc tcaagcaggt ctggcccggt ctctccgggg cccaggaacg
      961 ggccatcgcc gacgagttcc agcacgcgcg atcgatcgcg gtgaccctcg ccgacgagca
     1021 gatcgacgta cggggcccaa cggcaacggt gtcgtgccgc cgggcctacc ggctcgagac
     1081 gcgcgacggt cagcggctcg agtcggtgac gcgggccgag ttcggcctgc ggcaggcggg
     1141 cggcgggtgg gtcatcgagc acgtccgaca cgaacagcga tagtcagagt caccgttctc
     1201 ccgaacgcat cttccgacgg agcccagcat gaaacgcccc ctgattgcta tcgcgctcct
     1261 ggtttctctc gccgctcccg ccgccggaca gtcgcgcacc gaccagctgg cgctgctctt
     1321 ccaggaggtc ttcggcccga acggcctggt ggtgaacagc gagtcggtcc ttcccgacgg
     1381 ctcgacgcac tcggcgcatt tcaacagcgg gttccagtcg aacttcagcc agttcaacat
     1441 cgcgattgcc ggccagctcg cctcgctgcc gctgccgtcg ccggcctcgg gcttcactta
     1501 cacgttcgac accacgacgg gtaccttcgt ccgctcgacg cagagctatg gacccatcct
     1561 gaccgaccgc gccgagacga tcgggcgcgg acggttctcg ttcggcttca acgttcagtt
     1621 cttctccttc gacaccctcg aggggctgaa cctcggcggc atcccggccg tgttcaccca
     1681 cgacaatttc cagctcgggg gaggcctcgc cgacatcgtc agcaccgtca actcgatcga
     1741 ggcgtcggtg gggcagatga cggcggccgt gacctacggg gtgacggggc gcttcgacgt
     1801 gtcgctggcc gtgccgatgg tgcggacccg cctgagcgtg cgttccgacg ccagcattca
     1861 gcgcgtcggg accgaggacc cgtcgatcca ctttttccgc gatccggacg cgcctgaagg
     1921 ctacgggacg caccgccagt tcttcgccag cggcacggct accgggctgg gcgacgtgct
     1981 ggtgagggtg aagggcacgg cgttccgcga ggggagccgg ggcgtggcct tggggctcga
     2041 cgtgcggctg ccgaccggcg acgagcgcga tctcctgggg tcgggggcgc tcggcatcaa
     2101 gccgttcgcg gcggtctcgc tcggctgggg gcggttctcc ccgcacatca acgtgggcta
     2161 ccagtggaac ggaaggagcg tgctggccgg gaacgtgaag gccgacacgc gcgcgaagat
     2221 gcccgaccag ctgctctttt ccgtgggcgc cgacatcggc gtcagcgacc ggttctcgct
     2281 cgccgcggac gtcctcgggg cgcgcgccat cgactctccg cggctgtttc cgaccagctt
     2341 cacgccgcag ggctcgagcc aggcgtttcc cgacgtcgcg ttccgcacct cgaccttcac
     2401 gatgacggcg ggcgcggtcg gcttcaagat cgccctcgca aggcgcctgc tggccaactt
     2461 caacctgcgc ttcaggctga gcgatgccgg cctcgtcgat cgcgcgtcgc cgctcttcgg
     2521 ggtcgagtac gggttctagg ttctctcttt cgtgaccgcg tttcctgccg gtgcctgcag
     2581 cacaacagtt cgggttacta tccccgaatg cttctgctga agaacgccga ggttttcgac
     2641 ccggaaccga gggggcgcgc ggacatcctc gtggcgggcg ggcgcatcga gcgggtcgcg
     2701 cccggcctgt cgctgccggc cgacctctgc gacgtggtgg atgccggccc gtcgttcgtg
     2761 gttcccggtt tcatcgactg ccacgtacac gtgctgggcg ggggcggcga agggggctac
     2821 gccacccgca cgcccgagct gcggctctcg gacgcggtcg agggcggcgt gaccaccgtc
     2881 gtcgggtgcc tgggcaccga cggcgtgacg cgcagccttc cggcgctcct tgccaaggca
     2941 cggggcctcg aggaagaggg cctcaccacc ttcatctaca gcggtcacta cgcggtgccg
     3001 ctgcaggcga tgaccggcag catcgagcgc gacctgctgc tggtggacaa ggtcatcggg
     3061 gcgggcgagg tggccgtgtc cgaccaccgg tccacgcagc ccaccttcga cgagctggcg
     3121 cgcctcgccg ccgaggcgcg gcggggcggg atcctgtcgg ggaaggcggg cgtggtcaac
     3181 gtgcacctcg gcgacgggcc gcgcggcctg gccctgctcc ggcgcctggt cgaggagacc
     3241 gagatccccg cgtcgcagtt cctcccgacc cacatgaacc gcaacccgcg gctcttcgag
     3301 gaggcggtcg cgtgggtcgc gggcggcggg ctggccgact tcaccacctc cacggtgccg
     3361 gccttcgtcg aagagggcga ggtgaaggcc gcgctcgcgc tgcgccggat gatggaagcc
     3421 ggcgcgccgg tcgcgaacct caccttctcc tctgacgggc agggcagcct gccgagattc
     3481 gacggcgagg ggcggctcgt gggactcgac gtcggcaggg tgacgagcct gttcgcctcg
     3541 gtgcgggaag ccgtgctgga gcacggcgtg gcgctgccga cggcgctggc gctgatcacg
     3601 tcgaaccctg cgcgcatcct gaagctgcgc gggaagggcc gcgtcgccga gggagcggac
     3661 gcggacctcg tgctgctcga tccccacgcg ctggccgtca cgggcgtcat cgcccgcggc
     3721 cggtggctga tgcgcgacgg cgagccgctc gtcaggggga ccttcgaatg agcgtgcgcg
     3781 agcggcttcg gcgtctggcg atgccacaca cgctcgtggt ggtgatgtcg ctggtggtcc
     3841 tggtgctggc cctgtcgtgg atggtgccgt cgggcgagta ccagcgggtg caggcggaaa
     3901 ccagcgcggg gccgcgcacc gttaccgcgc ccggcaccta ccagcaggtc gagaagatct
     3961 atctcgggcc ccagatggtg ctcgaggcgc cgctcaaggg gttcgtggac ggcgccctgc
     4021 tcatcgcgtt cctcctgatg atcggcggga gcttcgcgat cttccaggag acgggggcgg
     4081 tcgacttcgg gattcgccgg ctcaccgggg cgctgacggc gcggccgcac ctcgaggggc
     4141 tgctgatccc ggccctgatg atcatcttct cgctcgccgg gtcggtgttc gggatgtcgg
     4201 aggaagtgat ccccttcatc ctgatcttca tcccgctggc ccgccggctg ggctacgact
     4261 cgatcgtggg cgtctcgatc ccgttcctcg gcgccgcggc gggcttcgcc gccgccttct
     4321 tcaacccgtt caccgtcggc atcgcccaaa gcatcgcggg gctcccgctc tactccggcc
     4381 tcggctaccg gctgttcacc tgggtcgtcg gcacggcggt gatggtcgcg tacgtgatgt
     4441 ggtacgcggg acgcgtccgg cgggaccctg acatcagccc cgtgcgggac atcgatctca
     4501 ggcgcgaggc ggccggctcg cacgcggggc cgcgcgaggg atgggacgcg cgccacgtgg
     4561 cgacgctcgc gctcttcgtc gcgtcgatgg tgctgctggt ggttggcgtc ctggcgtgga
     4621 agtggtacat cgaccagatc gcggtgctct tcttcgggat gggcatcgcg ctcgggttcg
     4681 ccgggggcat ggggcccagc cgcgtcgcgc gcaccttcgt cgccggcgcg aaggacatgg
     4741 tcggcgtcgt cttcatcatt gcctgcgcgc gtgccctgct cgtgatcgcg caggacgcga
     4801 agatcctcga caccatcctc tactgggcgt cgaacaccct ctcggcgctg ccccgcggcg
     4861 tgatcgcgca ggtgatgttc ctgatccagt gcgtcatcaa cttcttcatc cactcgggga
     4921 cggcgcaggc cgcgctcacc atgccgatca tggcgccgct cgccgacctc gtgggcatca
     4981 cgcggcagac cgccgtctac gcctaccagc tgtgcgagtt catcaacccg atcctgccga
     5041 ccagcgcggt gacgatggga gtgctcgggg cggcggggat cccgtgggag cggtgggcga
     5101 ggtggttcct gccgctgatg ctgatcctgg tggtgctcag cttcctgctg ctgatcccgc
     5161 cggtgctctg gttcgagtgg aggtagccgg atgcgtcgcg cgctcccgat cgtcgcggtc
     5221 gtcctggtgg cgctcgccgc gggcgggcag ggtttcagcc ccgtcccggc ggggcagggc
     5281 ccgaacccgg cccaggactt cgtctggccc ggcgaacggg agaagctgcc ggccccggcg
     5341 gccgacgcgg tggcatcgct gggagccgat cggctcgcca cccacgtcgc cttcctgtcg
     5401 gctccggccc tcggcggccg ggggctgggc gacgagggtc tcgaggccgc gatcgaatac
     5461 gtggccgcgc agctgtcgct ggcgggggtg gcgcccgcgc tcactgcaag cggcgcgcag
     5521 ccgctggagg cctactacca gccggtcacg ctgagagcgg tcagccggcc cggcggccgg
     5581 ctcacgatca gccggggcag cggggcaggt gctgtccaga cc
//
LOCUS       DAOPQX010000234         5609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15840_length_5609_cov_4.474613, whole genome shotgun sequence.
ACCESSION   DAOPQX010000234 DAOPQX010000000
VERSION     DAOPQX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15840_length_5609_cov_4.474613"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKK95_12025"
     CDS             <1..243
                     /locus_tag="PKK95_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOC18993.1"
                     /translation="HNALRAAALAVLHRGDMRVRQQAARLDALSPLAVLGRGYAVCWN
                     ADRSRILRDADAVADGEAVRVTLQKGEITATVTKRP"
     gene            255..548
                     /gene="xseB"
                     /locus_tag="PKK95_12030"
     CDS             255..548
                     /gene="xseB"
                     /locus_tag="PKK95_12030"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOC18994.1"
                     /translation="MDGTIKDFEAAIAELEAIVKKLEEGELPLEQSLSLYERGVHLSR
                     YCHARLEDAERRIEILDERGELRQAPPSLANPRNGEETGRNAASDRPDPERRR"
     gene            545..1618
                     /locus_tag="PKK95_12035"
     CDS             545..1618
                     /locus_tag="PKK95_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOC18995.1"
                     /translation="MTPRPAPPLDKYLRARRDLVDAALERFLPSPPACPAVIAEAMRY
                     SLFAGGKRLRPILALASAETIAADASLQAPATASLFAAAASPGRWDAPGAGDLEAGAA
                     ERLAIGLALPAACAIEMIHTYSLVHDDLPAMDDDTLRRGRPTSHVVYGEGIAILAGDG
                     LLTEAFALLARGPRDDGRGQRVEGGSPDPALPGLPERIQRTIAVIAEAAGACGMVGGQ
                     AIDLQAVGTARLASIEALRDMHARKTGALIKAAAVSGAVMAGAAAPVVAGIERYGAAI
                     GLAFQIVDDILDVEGSSADLGKTAGKDAAAGKPTYPALLGLDASRRLAAEAMDEARDT
                     LAAAGIQGSRLEEIARWVVERRR"
     gene            1618..2352
                     /locus_tag="PKK95_12040"
     CDS             1618..2352
                     /locus_tag="PKK95_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOC18996.1"
                     /translation="MKTRLDLVVVARGLAPSRERARALILAGQVRVNGALADKAGTMI
                     AEDAGVELVKPDHPYVGRGGLKLAHALDTFDIAVEGREALDIGASTGGFTDVLLRRGA
                     RRVVALDVGHGQLDWGLRNDPRVVVIEGLNARHLEPSHLPGPVDLVTIDVSFISLTQI
                     LPRVPALLAPAADVVALVKPQFEAGREEVGRKGIVRDPAVHERVVEKVGRAAEEVGLS
                     RAALTTSPITGSEGNVEFLLHLRARG"
     gene            2422..3306
                     /locus_tag="PKK95_12045"
     CDS             2422..3306
                     /locus_tag="PKK95_12045"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOC18997.1"
                     /translation="MAAISRVGIVAKPQLEEVATHLEQTAAWLEARRIEPVFEANTAG
                     LGRVGAGRRSASRDELPRLVDLLLVFGGDGTFLAVADRVAESGRDIPILGVNFGHLGF
                     LTEVAWTGMFASLEAAIAGEAAFDHRLVIRASVRRSGLETARRFALNDVVVTRGALSR
                     IIPLSVTVGGEFVATFHADGLIISSPTGSTAYNLSAGGPIVHPDVDALVLTPIAPHML
                     SNRPVVIPASTEVVVHPVALDAGQEAYVSIDGQSGQGLEPGDTLHVARHDHPIRLVES
                     SSHSYFEVLRKKLKWAER"
     gene            3329..4363
                     /gene="mltG"
                     /locus_tag="PKK95_12050"
     CDS             3329..4363
                     /gene="mltG"
                     /locus_tag="PKK95_12050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOC18998.1"
                     /translation="MRRLALVLFVLAAVAAGAWWALEWRLAQPYKGWEGEEVFVDILP
                     GEGTAVIGRKLIEAGVVPDRWTFRAALWRTGDARRLKAGEYRFDRPLGPREVVARLAR
                     GDVYLRAITFREGLTIREMARLWEEQGFGTASEFRRAAGRASLIHDLDPEARDLEGYL
                     FPDTYALPRRASVDTLIERMVAGFRRVFTEDLQEAARAQGFSVRQAVALAALVEKETA
                     KPEERPIVAGVYRQRLRIGMPLQADPTVIYALVAAGRYDGNLTRQGLQIDSPYNTYRY
                     PGLPPGPIAAPGRGSLEAAARPADVDYLYFVSRNDGSHVFSRTLDEHNRNVFRYQVKP
                     FRGRQGRGGR"
     gene            complement(4391..4822)
                     /gene="ruvX"
                     /locus_tag="PKK95_12055"
     CDS             complement(4391..4822)
                     /gene="ruvX"
                     /locus_tag="PKK95_12055"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOC18999.1"
                     /translation="MRVLGIDLGERRVGLAVSDPSGLIARPLAVLEARGEQRLHEVVR
                     AVRRLASEDDGLGEVVLGLPRRLDGSPHEQTMRALAFAEALRARIPQPVVLRDERLTS
                     VEAESRLALRDRDWRSRKRKLDAAAAAVILQEYLDERTAGR"
     gene            4880..>5609
                     /locus_tag="PKK95_12060"
     CDS             4880..>5609
                     /locus_tag="PKK95_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC19000.1"
                     /translation="MTKTILRAAAVLVAALVVAGACSSSPQPSSFKQKLVILGFDGMD
                     PRLVERFMDEGKLPTFKKLAAEGGFYRLETSTSPESPTSWASFAVGGNAGKHNIFDFL
                     VRDVKTYVPDLGMVRREMPSFLFDYIPTGKPKAVSLRGGTSFWVTAGQAGVRSSILTV
                     PVTFPPEEVPSGVMLSGLPLPDIRGTVGTFYYFASDLSRYEEGNTEMGGILKRLVFEG
                     DLARTELVGPSNPIVSAKMQEVRQK"
BASE COUNT          793 a         2015 c         2028 g          773 t
ORIGIN      
        1 cacaacgcgc tccgggcggc ggcgctggcg gtactccacc ggggcgacat gcgcgtgcgg
       61 cagcaggcgg cccgcctcga cgcgctcagc ccgctcgccg tgctcgggcg cgggtatgcc
      121 gtctgctgga acgccgaccg cagccggatc ctccgcgacg ccgatgcggt agccgacggc
      181 gaggcggtcc gcgtcacgct gcagaagggc gagatcaccg cgacggtcac caagcgcccc
      241 tgaggcccgc gaccatggat ggcaccatca aggacttcga ggcggccatc gccgagctcg
      301 aggccatcgt gaagaagctc gaagagggcg aactgcccct cgagcagtcg ctctcgctct
      361 acgagcgcgg cgtccacctc tcgcgctact gccacgcacg cctcgaggat gccgagcggc
      421 gcatcgagat cctcgacgag cgcggcgagc tgcgccaggc gccgccgtcg ctggccaacc
      481 cgcgcaacgg cgaggaaacc ggccgcaacg ccgccagcga tcgcccggac ccggagcgtc
      541 ggcggtgacg ccaaggccgg cgccgcccct cgacaagtac ctccgcgcgc ggcgcgacct
      601 ggtggacgcg gcgctcgagc gcttcctgcc ctcgcctccc gcctgccccg cggtcatcgc
      661 cgaggcgatg cgctacagcc tgttcgccgg cggcaagcga ctgcgcccga tcctcgcgct
      721 ggcctcggcc gagacgatcg ccgccgacgc gtcgctgcag gcgcccgcga cggcaagcct
      781 cttcgccgcc gccgcctcgc cgggacggtg ggacgcgccg ggcgccggcg acctcgaggc
      841 aggcgcagcg gagcgcctcg cgatcggcct cgcgctgccc gccgcctgcg cgatcgagat
      901 gattcacacc tactcgctgg tccacgacga tctgcccgcc atggacgacg acaccttgcg
      961 gcggggacgg cccaccagcc acgtggtcta cggggagggg atcgccattc tcgccggcga
     1021 cggcctgctg accgaggcgt tcgcgctgct cgcacgcggc ccgcgcgacg atggccgggg
     1081 acagagggtg gagggcggaa gtccggaccc ggcgctgccg gggctgccgg agcgcatcca
     1141 gcggacgatc gcggtcatcg ccgaggccgc aggggcgtgc gggatggtgg gcggccaggc
     1201 gatcgacctg caggcggtcg gcaccgcgcg cctggcctcg atcgaggcgc tgcgcgacat
     1261 gcacgcccgg aagacgggcg ccctcatcaa ggccgcggcc gtatcgggcg ccgtgatggc
     1321 cggcgcggcc gcaccggtcg tcgccgggat cgagcggtac ggcgccgcga tcggcctggc
     1381 cttccagatc gtggacgaca tcctcgacgt cgaggggtcg agcgccgacc tgggcaagac
     1441 cgccggaaag gacgcggcgg ccggcaagcc gacctacccc gccctcctcg gcctcgacgc
     1501 gtcgcggcgg cttgccgccg aggcgatgga cgaggcccgc gacacgctgg ccgcggccgg
     1561 catccagggc agccgcctcg aggagatcgc gcggtgggtg gtggaacgac gccgctgatg
     1621 aaaacccgtc tcgacctcgt cgtcgtcgca cgcggcctgg ccccgtcgcg cgagcgggcg
     1681 cgcgcgctca tcctcgcggg gcaggtgcga gtgaacggcg cgctggccga caaggccggg
     1741 acgatgatcg ccgaggacgc gggggtggag ctggtaaagc cggaccaccc ttacgtcggg
     1801 cgcggcgggt tgaagctggc gcacgcgctc gacacgttcg atatcgcggt cgaggggcgc
     1861 gaggcgctcg acatcggggc ctcgaccggc gggttcaccg acgtcctgct tcgacggggc
     1921 gcgcggcgcg tcgtcgcgct cgacgtgggc cacggtcagc tcgactgggg gctgcgcaac
     1981 gacccccggg tggtggtgat cgagggcttg aacgcgcggc acctcgaacc gtcgcacctg
     2041 cccgggccgg ttgacctggt gacgatcgac gtctctttca tttcgctgac gcagatcctg
     2101 ccgcgcgtcc cggcgctgct ggcccctgcg gccgacgtcg tggcactggt caagccgcag
     2161 ttcgaggccg ggcgcgagga ggtcgggcgc aagggtatcg tgcgcgaccc ggcggtccac
     2221 gaacgtgtcg tcgagaaggt ggggcgagcc gccgaagaag tgggcctgtc gcgcgccgct
     2281 ctgacgacct cgccgatcac cgggtcggag gggaacgtgg agttcctgct gcatctgagg
     2341 gctaggggct aggagctagg ggctaggggc taggggctag gggctagcag gcttctgtca
     2401 gcaaccggcg tggaggccgt gatggcagcc atctcgcggg tcgggatcgt cgccaagccg
     2461 cagctggaag aggtggccac acacctcgaa cagacggccg cgtggctcga ggcgcgccgc
     2521 atcgagccgg tcttcgaggc caacacggcc gggctggggc gggttggcgc ggggcgccgc
     2581 tcggcgagcc gggacgagct gccgcggctg gtggacctgc tgctcgtctt cggcggggac
     2641 gggacgttcc tggcggtcgc cgaccgcgtg gccgagtcgg ggcgcgacat cccgatcctc
     2701 ggcgtgaact tcggccacct cggcttcctc accgaggtgg cgtggacagg gatgttcgcc
     2761 tcgctcgagg cggccatcgc gggcgaggcg gccttcgacc accggctggt gatccgggcc
     2821 tcggtccggc ggtcgggcct cgagacggcc cgccgcttcg ccctcaacga cgtcgtggtg
     2881 acgcgcgggg cgctgtcgcg catcatcccg ctgtcggtca cggtgggcgg cgagttcgtg
     2941 gccaccttcc acgccgacgg cctcatcatc tcgagcccca ccgggtccac cgcctacaac
     3001 ctgtcggccg gcggcccgat cgtgcacccg gacgtggacg ccctggtgct cacgccgatc
     3061 gccccccaca tgctgagcaa ccggccggtc gtgatcccgg cctccaccga ggtggtggtg
     3121 caccccgtcg ccctggacgc cggccaggag gcatacgtct cgatcgacgg ccagagcggc
     3181 cagggcctcg aaccgggcga cacgctgcac gtggcccgcc acgatcatcc gatccgcctg
     3241 gtggagtcgt cttcgcacag ctacttcgag gtgctgcgga agaagctgaa gtgggcggag
     3301 cggtaaccgc acggtttacc attgatccat gcgacgcctc gcgctggtgc tcttcgttct
     3361 cgcggcggtg gcggccggcg cctggtgggc cctcgagtgg cggctcgctc agccctacaa
     3421 ggggtgggag ggcgaggagg tcttcgtcga catcctgcca ggcgagggga ccgcagtcat
     3481 cggccgcaag ttgatcgagg ccggcgtcgt gcccgatcgg tggacgttcc gggctgcact
     3541 ctggcgcacg ggagacgccc ggcggctcaa ggccggcgag taccggttcg accggcccct
     3601 cggcccgcgc gaggtggtcg cgcggctggc gcggggcgac gtctacctgc gggcgatcac
     3661 gtttcgcgaa gggctgacca tccgcgagat ggcgcgcctc tgggaagagc aggggttcgg
     3721 cacggcgtcg gagttccgca gggcggccgg gcgcgcatcc ctgatccacg acctcgatcc
     3781 cgaggcccgc gacctcgagg gctacctgtt ccccgacacc tacgcgctgc ccaggcgcgc
     3841 gagcgtggac acgctcatcg agcggatggt cgctggcttc cgccgcgtct tcacggagga
     3901 cctgcaggaa gcggcgcgcg cgcaggggtt cagcgtgcgc caggcggtgg cactcgccgc
     3961 gctcgtcgag aaggagaccg ccaagcccga ggagaggccg atcgtggccg gcgtctaccg
     4021 ccagcgcctc cggatcggga tgccgctgca ggccgacccg accgtcatct acgcgctggt
     4081 cgccgccggc cgctacgacg gcaacctgac ccgccagggg ctgcagatcg attcacccta
     4141 caacacctac cgctacccgg gacttccgcc gggcccgatc gccgcgccgg gacgaggatc
     4201 gctcgaggcg gcggctcggc cggcggacgt cgattacctg tacttcgtca gccgcaacga
     4261 cggctcccac gttttctccc gcacgctcga cgagcacaac cgcaacgtct tccgctacca
     4321 ggtcaagccc ttccggggcc gtcaggggcg gggcggacgc tgaaggagaa gcgtgtaatg
     4381 gccgcgttgt tcaccggcct gccgtccgct cgtccaggta ctcctggagg atcaccgcgg
     4441 cggcggccgc gtcgagcttc ctcttgcggc tgcgccagtc gcggtcgcgc aacgcgagcc
     4501 ggctctcggc ctccacgctc gtcagccgct cgtccctgag gacgaccggc tgcgggatgc
     4561 gagcccggag cgcctcggcg aacgccagcg cccgcatcgt ctgctcgtgc ggcgagccgt
     4621 cgaggcggcg cggcagcccc agcaccacct cccccaaacc atcgtcctca gacgccaggc
     4681 gtcgcacggc gcgcaccacc tcgtggagcc gctgctcgcc gcgtgcctcc agcaccgcca
     4741 ggggccgcgc gatgagaccc gaggggtcgg agacggccag tcccacgcgc cgctcgccaa
     4801 ggtcgatgcc cagcacccgc acggggggga tgatatactc cgggatggaa taccgtccac
     4861 aacggtccgc attccaacga tgaccaagac catcctccgc gcagccgccg tgctggtggc
     4921 cgcactcgtc gttgcgggcg cctgctcctc gtcgccccag ccctcgtcgt tcaagcagaa
     4981 gctggtgatc ctcgggttcg acgggatgga cccgcggctg gtggagcgct tcatggacga
     5041 gggcaagctg cccaccttca agaagctcgc ggccgagggc ggcttctacc ggctcgagac
     5101 gtccacctcg cccgagtcgc ccacctcgtg ggcctcgttc gccgtgggcg gcaacgccgg
     5161 caagcacaac atcttcgact tcctcgtccg cgacgtgaag acctacgtgc ccgacctggg
     5221 gatggtccgg cgcgagatgc cgtccttcct cttcgactac atcccgacgg gcaagccgaa
     5281 ggcggtctcg ctgcggggcg gcacgtcgtt ctgggtgacg gcgggacagg ccggcgtgcg
     5341 gtcgtcgatc ctcacggttc cggtcacctt cccgcccgag gaggtcccct ccggggtgat
     5401 gctctcgggg ctgccgctgc ccgacatccg cggcacggtg ggaaccttct actacttcgc
     5461 gagcgacctg agccgctacg aagaaggcaa caccgagatg ggcggcatcc tgaagcggct
     5521 cgtcttcgag ggcgacctcg cgcgcaccga gctggtcggc ccctcgaacc ccatcgtctc
     5581 ggcgaagatg caggaggtgc ggcagaagc
//
LOCUS       DAOPQX010000235         5597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15872_length_5597_cov_3.945327, whole genome shotgun sequence.
ACCESSION   DAOPQX010000235 DAOPQX010000000
VERSION     DAOPQX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15872_length_5597_cov_3.945327"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..705
                     /locus_tag="PKK95_12065"
     CDS             202..705
                     /locus_tag="PKK95_12065"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC19001.1"
                     /translation="MKVSELFLAELDREADRSRRMLQLLPEGKYDWKPHERSMSFGYL
                     AQLVGTMFSWVTATVKQDELDLQPKGGPQAPPRLDTSAEQVQALEKAVADARAALQST
                     TDEHLATPWRLLVAGNVVWEAPRHEVIRETFNHLAHHRGQLTVYYRLLGVKVPALYGP
                     SADERPF"
     gene            complement(809..1594)
                     /gene="mtgA"
                     /locus_tag="PKK95_12070"
     CDS             complement(809..1594)
                     /gene="mtgA"
                     /locus_tag="PKK95_12070"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOC19002.1"
                     /translation="MRSPFRGRPDLHFRRRPASRLRLAIVAIVGSAACFLATSVAITL
                     SLRWVPLSWSALMAERQLAALAQGKKYVPAYSWVGWDRIAPHAPLAVIAAEDQRFADH
                     FGFDIEAIQKAIGEQERGRRRRVRGASTISQQVAKNVFLWPGRSYVRKGLEAYFTLLI
                     ETLWPKRRILEVYLNVAEMGDGVFGVEAAARRFFGKPASRLTAGEAALLAAVLPNPHR
                     FRANRPSAYVAGRRNWILGQMAQLGGAQYLVDRGFHPGAVPKR"
     gene            complement(1756..2745)
                     /locus_tag="PKK95_12075"
     CDS             complement(1756..2745)
                     /locus_tag="PKK95_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC19003.1"
                     /translation="MKIGARELLMAWGRLLRGGSPLLSIEVTRQCPLHCRGCYAHVEG
                     HAGSSGAVAELHGEALVARVLALVARHRPQHVSIVGGEPLLRRRELDALLPRLAALGV
                     PTMVVTSGVLPIPMEWDSLGRLVVTVSVDGLAPDHARRRAPATYERILRNIEGRRVSL
                     HWTVVHGMLSRAYFEENLAFWSARPEVQHLLVSAYTPQRGERTPEMLTPEDRLTLASL
                     LPALKVRYPKLLTSNEIARAYLHPPRDPAHCLFARVSRAYAPDLATEVVPCLIGGNPD
                     CTQCGCAVPAALHGIAATRIKGPLRVGHLIATTTSIAAAVNRVGPSRRLRGAA"
     gene            complement(2771..3961)
                     /locus_tag="PKK95_12080"
     CDS             complement(2771..3961)
                     /locus_tag="PKK95_12080"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC19004.1"
                     /translation="MPPPAWPDAQPLPEPPFPRHPELLGEERSGPVVQDRHWSLPLIR
                     RKVNGWLVPYVKSRVLPGSFHPILAYLFTDWKCNVDCHYCPSWDNRTPGMTEDVARRS
                     IDWLESTTCRVLAVMGGEPLVRPAFVHKVTDYAASKGFWVYIGTNARLLKPAVVDRLG
                     DAGMAVFNVAMDTVDPKPGLPKALNPVRRQFEYLLRKQYDYGYMVFFNICICRTNMDD
                     VRDLTEIAHEYRIATDYHVCESPMYEQPHFKHLHDNGVFLRREDWPKVDELVDWLIEK
                     NKAGYAMVNSIKRLNDMKDFLRGRLEPWNCRAGLNSVVIRTDGSVAPCFPLYTAKVDW
                     GHIGSPRFDAARLEAMKVECQRNCFSTLQHNLGFCYNDRRVLHWVARQAARGFRSGAR
                     CFED"
     gene            complement(4321..>5597)
                     /locus_tag="PKK95_12085"
     CDS             complement(4321..>5597)
                     /locus_tag="PKK95_12085"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19005.1"
                     /translation="RAGLRRQVVLLTDGEVSNEAAVIELAEMHRAHTRIFTFGIGYGA
                     SEYLVRSLARVTLGQAEFIHPGERIEPKVLRQFARLGRPAVRDVKVEWKGAAARLQAP
                     HRAPTIFDGEAAVLYARLEPGAAVSVALGGDAEGQPVSWSLGVDIGEARPGTLLATLA
                     ARAAIRDLEEGTSALHDRPRGSAQRGRREKRVSEAIVALALAHHLASSETSFVAIERR
                     EGAEEEPAAELRRVPVQLTAGWGGLDRARGAVQAQLLSAVSLDQNLRYCMSVMERSPD
                     VSACLSSPASGETSGPRVDDLLRRYERSTSRRSPAPFASGSQPERPHVLVARLQQADG
                     SWALEGRLADTIGASLRALESLAQTIGHDEEARAVVATLAALVFLDLRVGDARDEWRL
                     LAVKAEAWLARVLARRPDVDRADLERLLTSLL"
BASE COUNT          865 a         1986 c         1928 g          818 t
ORIGIN      
        1 cgaactgccg ccacagggct gcggcgagcc ggccatcacc gcgatggcgg ccgttctggc
       61 caacgcgctc ttcgacgcca ccggcgtgcg tgcgatccgc ctgccgctgg tgctgccggc
      121 agcccgctag gcggaagcgg aggcggtccg ttccttgctg tccgacgggc ggctgcaccc
      181 gtcatccaca aggaggttgc cgtgaaggtc agcgaactgt tccttgcaga gctggaccgc
      241 gaagccgatc gctcgcgccg catgctgcag ctgctccccg agggcaagta cgactggaag
      301 ccgcacgagc gatccatgtc gttcggctac ctggcccagc tcgtcggcac catgttctcg
      361 tgggtaacgg cgaccgtgaa gcaggacgaa ctcgacctgc aaccgaaggg ggggccgcag
      421 gcgcccccgc ggctggacac gagcgcggaa caggtccagg cgctggagaa agcggtcgcc
      481 gatgcgcggg cggccctcca gtccaccacc gacgagcacc tggcgacccc gtggcggctg
      541 cttgtggccg gcaacgtcgt gtgggaggcg ccgcgccacg aggtaatccg cgagaccttc
      601 aaccacctgg cgcaccaccg cggccagctc acggtctact accgcctgct gggcgtgaag
      661 gtgcccgcac tctacggacc gtcggccgac gaacggccct tctgaagggg gccgtaccgg
      721 aaatgtgacg gatttcacct tttcggggcc gtaccagaaa cgtgacgttt ttcacctttt
      781 cggtacggcc ccgggggtgt gacgaacttc accgtttcgg tacggccccg gggtgaaagc
      841 cccgatccac caggtactgc gcgccgccaa gctgcgccat ctggccgagg atccagttgc
      901 ggcggccggc gacgtaggcc gacgggcggt tggcgcggaa gcggtgagga ttcggcagca
      961 cggcggcgag cagcgccgcc tcgcccgcgg tgaggcgcga ggccggcttg ccgaagaagc
     1021 gccgcgccgc cgcctccacc ccgaagacgc cgtctcccat ctcggcaacg ttcaggtaga
     1081 cctcgaggat gcggcgcttc ggccagagcg tctcgatcaa cagcgtgaag tacgcctcga
     1141 gccccttcct cacgtagctg cgccccggcc agagaaagac gttcttggcc acctgctgcg
     1201 agatggtgct cgcgccgcgt acgcggcgcc ggcgcccgcg ctcctgctcg ccgatggcct
     1261 tctggatggc ctcgatgtcg aagccgaagt gatcggcgaa gcgctggtcc tcggctgcga
     1321 tcaccgccag cggcgcgtgc ggcgcaatgc ggtcccagcc cacccaggag taggccggca
     1381 cgtacttctt cccctgcgcc agcgccgcca gctgccgctc ggccatcagg gccgaccagg
     1441 acagcggaac ccaccgaagc gacagggtga ttgcgaccga ggtggcaagg aaacaggccg
     1501 cacttcccac gatcgcgacg atggcgaggc ggagacgcga ggcggggcgg cgcctgaagt
     1561 gcaggtccgg acggccgcgg aagggagatc tcacccgacg ggcctcgtcg aaaccgaaag
     1621 gcgggccacg cggcgcgtgt gcaccatgac gcggggattg tagatcacgc gggtcggggg
     1681 ttggcgcgcg cccgcgcctt gccggcttcg tcccgggcat gtcgggcgcc gacgcgtcgt
     1741 agaacagccg tgggatcacg cggcgccgcg cagccgcctc gaaggaccca cgcggttcac
     1801 cgccgccgcg atcgacgtgg tcgtcgcaat gaggtgcccg acgcgcagcg gtcccttgat
     1861 gcgggtggcg gcgatgccgt gcagtgccgc ggggaccgcg caaccgcact gcgtgcagtc
     1921 cggattgccg ccgatcaggc acggcacgac ctcggtggcc aggtcggggg cgtaggcccg
     1981 gctcacccgc gcgaagaggc agtgcgcggg atcgcgcggg gggtgcaggt aggcccgggc
     2041 gatttcgttg gaggtcaaga gcttcgggta gcgcaccttc agggcgggga gcagtgaggc
     2101 cagcgtcaac cggtcctccg gagtcagcat ctccggcgtg cgctctcccc gctgcggcgt
     2161 gtaggcgctc accaggaggt gctggacctc gggcctggcg ctccagaacg ccaggttctc
     2221 ctcgaagtag gcacgcgaga gcatcccgtg cacgacggtc cagtgcaggc tcactctccg
     2281 gccctcgatg ttccggagaa tgcgctcgta ggtggcgggc gcccggcgcc gcgcgtgatc
     2341 cggggccagg ccgtcgaccg agacagtgac gaccagccgg ccgaggctgt cccattccat
     2401 tggaatcggc aagacgccgc tcgtgacgac catcgtcggg acgccaaggg ccgccagccg
     2461 gggcagcagc gcgtccagct cgcggcgccg cagcagcggc tcaccgccca cgatcgagac
     2521 gtgctgcggg cggtgccggg cgacgagagc gaggacgcgc gcgaccagcg cctcgccgtg
     2581 cagttccgcg acggcacccg aggagccggc gtgcccctcg acgtgggcgt agcagccgcg
     2641 gcagtgcagg gggcactgac gcgtaacctc gatcgacagc agtggcgagc cgccgcgaag
     2701 cagcctgccc caggccatca gaagctcgcg ggctcctatc ttcatggcgc tccgccctgg
     2761 aggctcatgg tcagtcttcg aagcagcggg cacccgacct gaagcctcgc gccgcctgcc
     2821 tggcaaccca gtgcagcacg cggcggtcgt tgtagcagaa tccgaggttg tgctgcagcg
     2881 tcgagaagca gttgcgctgg cactcgacct tcatcgcttc caggcgggcc gcgtcgaatc
     2941 tcggcgagcc gatgtgaccc cagtcgacct tcgccgtgta gagcggaaag cacggcgcca
     3001 cgctcccgtc ggtccggatg acgaccgagt tcagccccgc gcggcagttc cacggctcga
     3061 gccgcccacg caggaaatcc ttcatgtcgt tcagccgctt gatcgaattg accatcgcat
     3121 agccggcctt gttcttctcg atgagccagt cgaccagctc gtccaccttc ggccagtcct
     3181 cgcggcggag gaacacgccg ttgtcgtgga ggtgcttgaa gtgcggctgc tcgtacatcg
     3241 gcgactcgca gacgtgatag tcggtggcga tccggtactc gtgcgcaatc tcggtcaggt
     3301 cccgcacgtc gtccatgttg gtccggcaga tgcagatgtt gaagaacacc atgtagccgt
     3361 agtcgtactg cttgcgcagc aggtactcga actggcggcg caccgggttg agcgccttcg
     3421 ggaggccggg cttcggatcg accgtgtcca tcgcaacgtt gaacaccgcc atccccgcgt
     3481 cgccgaggcg atcgaccacc gccggcttca gcagccgggc gttggtcccg atgtagaccc
     3541 agaagccctt cgacgccgcg tagtcggtca ccttgtgcac gaacgccggc cgcacgaggg
     3601 gctccccgcc catgaccgcc agcacgcggc acgtcgtgct ctcgagccag tcgatggagc
     3661 gcctggccac gtcctcggtc atcccgggcg tgcggttgtc ccacgacggg cagtagtggc
     3721 agtcgacgtt gcacttccag tcggtgaaca ggtaggcgag gatcgggtga aagctccccg
     3781 gcagcaccct cgacttgacg tacgggacga gccagccgtt caccttcctc ctgatcagcg
     3841 gcagcgacca gtgccggtcc tgcaccaccg ggcccgagcg ctcctcgccg agcaactcgg
     3901 ggtgccgcgg gaacggcggt tctggaagcg gctgcgcgtc gggccaggcc ggtggcggca
     3961 cgctccgggg gtcagagccc tccagcccct tgatccatct gtccagccga agcggagcga
     4021 ccggttcccc tacccctttg ctctcgaggg caattgcgtc agcggatgag ctggcgggcg
     4081 tggaatccat atgcgtctcc acgggcgccg ccttcgaatg gtgaggatca ggaaggatgg
     4141 gaagcgcgaa gcggacgcgg cggacgccgg ccgaaccttt tcaagattag tgccgcggtc
     4201 gacgcggtgc gcggaggcgg ttctccaagc ctgccagggc tttgcgtcga aggacgggaa
     4261 ggctgaacgc gccgcggcgg caaatcgcta cggcgcgtcc ggggaaaatg tcgcgtagcc
     4321 ctacaggagc gaggttagca ggcgctcaag gtcggcgcga tcgacgtcgg gcctcctggc
     4381 gagcacgcgc gccagccatg cctcggcctt gacggccagc agccgccact cgtcgcgggc
     4441 gtccccaacc cgcagatcga ggaacaccag cgccgccagc gtggcgacca cggctcgcgc
     4501 ctcctcgtcg tgcccgatgg tctgggccag cgattcgagc gcgcgcaggc tcgcgccgat
     4561 cgtgtcggcg agccggcctt caagggccca cgagccgtcc gcctgctgca gccgcgccac
     4621 caggacgtga gggcgctccg gctggctccc cgacgcgaaa ggcgcgggtg agcgtcgcga
     4681 tgtgcttcgt tcgtacctgc ggagcaggtc gtcgacgcga ggccccgacg tctccccaga
     4741 agccggcgag ctcaggcatg cactcacgtc cggcgacctt tccatgacgc tcatgcagta
     4801 gcgtaggttc tggtcaagcg agaccgctga caggagttgc gcctggacgg cgccgcgcgc
     4861 cctgtccaac ccgccccacc cggccgtcag ctgcaccggc acacggcgca actccgcggc
     4921 cggctcctcc tcggccccct cgcgccgctc gatcgcgaca aacgacgtct cgctcgacgc
     4981 gaggtgatgc gcgagcgcca gcgccacgat cgcctcgctc acacgcttct ctcgccgtcc
     5041 gcgctgcgcg ctcccgcgcg gccggtcgtg cagcgcgctc gtcccctcct ccaggtcgcg
     5101 gatggcggcg cgcgccgcga gcgtcgccag cagggtgccc ggccgggcct cgccgatgtc
     5161 caccccgagg ctccagctca ccggctgccc ttccgcgtcg ccgccaagcg cgaccgacac
     5221 cgcggcgccg ggttccagcc tcgcgtacag gaccgccgcc tcgccgtcga agattgtggg
     5281 cgcgcggtgc ggcgcctgca gcctggccgc cgcgcccttc cattccacct tcacgtcgcg
     5341 cacggcaggc cggccgaggc gcgcaaactg ccgcagcacc ttcggctcga tccgctcgcc
     5401 ggggtggatg aactcggcct gcccgagcgt cacccgcgcc agcgagcgca ccaggtattc
     5461 gctcgcgccg tagccgatgc cgaacgtgaa gatgcgcgtg tgcgcccggt gcatctccgc
     5521 cagctcgatg acggccgcct cgttgctcac ttccccgtcg gtcagcagca ccacctgccg
     5581 gcgaaggccg gcccgcg
//
LOCUS       DAOPQX010000236         5595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15878_length_5595_cov_4.691697, whole genome shotgun sequence.
ACCESSION   DAOPQX010000236 DAOPQX010000000
VERSION     DAOPQX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15878_length_5595_cov_4.691697"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKK95_12090"
     CDS             complement(<1..298)
                     /locus_tag="PKK95_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOC19006.1"
                     /translation="MPGSFLRELDARVLVCDGAMGTMLYAKGVFVNRCFESLNLAQPD
                     LVAEVHLGYARAGADVLETNTFGANRVKLASFGLSDKVRAINLAGARIARHAARE"
     gene            complement(318..731)
                     /locus_tag="PKK95_12095"
     CDS             complement(318..731)
                     /locus_tag="PKK95_12095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOC19007.1"
                     /translation="MIRALVVGFSTTFRYLFRKPVTISYPEQKMPMFPKYRGKQVLMR
                     DEQGLEKCVACGLCAVACPADAIYLEPAENDGTVKAGPRYAKVYQIHKTRCIFCGYCE
                     EACPVSAIFMGKDYELAVYSKDDFVWDKADLLVSS"
     gene            794..2110
                     /locus_tag="PKK95_12100"
     CDS             794..2110
                     /locus_tag="PKK95_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC19008.1"
                     /translation="MPPTPGLLSPIQILLTTLVVKLALAALLGIVLVRYRRFRAILLS
                     DRRAWPERLIFAAALGIPVMAGVVARLLLSYKAADLSLEASLVAGLIAGPYAGGIVGF
                     LAGFPPVLAGEFAALPFAVGCGFAGGGFGALCPRDAVWKFSPFVFTSLHERFWKLARR
                     LQVDWQVVLFLVPVGFEFLRLAVANRIGTAYLFSMNPHGLGETVAVILATVLGVAIPI
                     KIWDNARIEHRLVEQEKLLMAARIQALSSQINPHFLFNALNSISSLVRSQPEAARELI
                     AKLSNLLRERLQSRDQFVTLREELDTIDQYLAIEAVRFGPQLRVAKRIEPEALDAIVP
                     SMILQPLVENSIKHGLTKKIGGGTISLRAFRQNGRSIIEVADDGLGMTPADLENALTA
                     GIGLSNVSERLRVTYGDHSALQLTSAQGQGTCARLEIPEMAVAMKS"
     gene            2191..2952
                     /locus_tag="PKK95_12105"
     CDS             2191..2952
                     /locus_tag="PKK95_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOC19009.1"
                     /translation="MRALVVDDEQLAREELCFQLARLEDVEVVGQAGNGVEALEVAGR
                     LRPDVIFLDIQMPGLGGFEVARDLLASDNPGQVVFVTAFDQYAIEAFEVNAVDYLLKP
                     VEPGRLEVAVQRVRRRLQPSAPLNEQLEKIVRMMGRAPGRRGQVAVKVGDRFLLVQAE
                     DVIYASVNGDTITIVTGQLSGTSNYRTLDELQGRLDPEVFWRVHRSHLVNINKIKEIV
                     PWFSRNYILRMKDGKGTEIPVSRSQTRRLREYLKL"
     gene            3111..3620
                     /gene="lepB"
                     /locus_tag="PKK95_12110"
     CDS             3111..3620
                     /gene="lepB"
                     /locus_tag="PKK95_12110"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOC19010.1"
                     /translation="MRDELWAWVKTVASAAVYATLIVTFGFQVARVEGMSMAPTLEDQ
                     DRLIVNKLIYRLSAPRRGDIVMLYYPVNPDKSFVKRVIAEEGDFVRIVDGRVYVNDVP
                     LPDDYVPMEYRSHDDWGPSVIQEGYYFVMGDHRNNSSDSRHWGPVPKRYIIGKVQVRW
                     WPLPEAKLF"
     gene            complement(3617..5437)
                     /locus_tag="PKK95_12115"
     CDS             complement(3617..5437)
                     /locus_tag="PKK95_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOC19011.1"
                     /translation="MTSSDQFTLATLPFHFRDRFQRPAMVGRCREGGCTTASTQEFFE
                     GIRALSLGLTALGLRRGDRVAIISDSRPEWVMTDLATLSAGAVTVPIYPTLSAGQARF
                     ILKESGCRIAVVSNAVQVTKLNEVVRDLPDLEAIVAMDGEAVTPRVRGVSLPDVTAWG
                     RAQLASDAGAAGRYEEVARQVAPSDLATIVYTSGTTGDPKGVMLSHSNIVSNILATTG
                     WITLEPEDVALSFLPLSHVFERVVFFRFLYSGVPVYFAEALTTVARDLVQVRPTVMTG
                     VPRVFEKVHAAIADVVGKMHGPRRRLADWALEAGHEAARLQLAGESIPATLGLKRRAA
                     DRLVLGKMRARLGGRLRFVVSGSAPLSKQVGEFFFAIGLPIYEAYGLTETSPGITGNP
                     PDAPRLGTVGKALPGVEVAIAPDGEILVRGPNVMQGYYERPEATAEVLVDGWLRTGDI
                     GQLSADGYLTITDRKKDLIVTSGGKKIAPQPLENQLKADPLVAEAVIIGEQRKFPAAL
                     IVPDFPRLKAWAERVGLPPLAGEQLAERPEVQRLYQDILDRLNESLAQFERVKRFAVL
                     PSEFTMERGELTPTMKVRRQVVEQRWKPLIDRLYEWPIRP"
BASE COUNT          880 a         1945 c         1886 g          884 t
ORIGIN      
        1 gttcgcgcgc ggcgtgccgc gcgatccggg cccccgccag gttgatcgcg cgcaccttgt
       61 cggacagccc gaacgaggcc agcttcaccc ggttggcgcc gaaggtgttg gtctcgagca
      121 cgtcggcgcc ggcgcgcgcg tacccgaggt gcacctcggc caccaaatcg ggctgcgcca
      181 ggttcagcga ctcgaaacac cggttgacga agacgccctt ggcgtacagc atcgtcccca
      241 tcgcgccgtc gcagacgaga acgcgggcgt cgagctcgcg caggaaggaa ccgggcatca
      301 ggaatggttg ggagatctca cgacgacacc agcaggtcgg ccttgtccca cacgaagtcg
      361 tccttgctgt agaccgccag ttcgtagtcc ttgcccatga agatcgccga gacggggcag
      421 gcctcctcgc aatacccgca gaagatgcag cgcgtcttgt ggatctggta caccttggcg
      481 tagcgggggc ccgccttcac cgtgccgtcg ttctcggccg gctccaggta gatcgcatcc
      541 gccgggcacg ccaccgcgca cagcccgcac gccacgcact tctcgagccc ctgctcgtcc
      601 cgcatcagca cctgcttccc ccggtacttg gggaacatcg gcatcttctg ctcgggatag
      661 ctgatcgtga ccggcttgcg gaacaggtac cgaaaggtcg tcgagaagcc gaccaccagc
      721 gcgcgtatca tcggctcgcc tccggggacg aagaggatcg ggtggcccga atcgttttat
      781 catagcatcg gctgtgccac cgacacccgg gctgctgtcg ccgattcaga tcctgttgac
      841 gacgctggtc gtcaagctgg cgctcgcggc gctgctggga atcgttctcg tccgctaccg
      901 ccgcttccgc gccatcctgc tgagcgaccg gcgcgcatgg cccgagcgcc tcatctttgc
      961 cgccgccctc ggcatcccgg tgatggccgg cgtcgtggcc cggctgctcc tctcctacaa
     1021 ggccgccgac ctctcgctcg aggccagcct cgtggccggc ctgattgccg gcccctacgc
     1081 cggcggcatc gtcggcttcc tcgccgggtt cccgccggtc ctcgcgggcg agttcgccgc
     1141 ccttcccttc gcggttggct gcgggttcgc cggcggcggc ttcggcgccc tgtgcccgcg
     1201 cgacgccgtg tggaagttct ctcccttcgt cttcacgagc ctgcacgagc gcttctggaa
     1261 gctggcccgc cggctgcaag tggactggca ggtcgtcctg ttccttgtcc cggtggggtt
     1321 cgagttcctg cggctcgccg tcgcgaaccg catcggcacc gcctacctgt tcagcatgaa
     1381 cccgcacggc ctcggtgaaa cggtcgcggt catcctggcg accgtgctcg gcgtcgcgat
     1441 cccgatcaag atctgggaca acgcgcggat cgagcaccgc ctcgtcgagc aggagaagct
     1501 gctgatggcc gcgcgcatcc aggccctgtc gagccagatc aacccccact tcctgttcaa
     1561 cgccctgaac tccatctcct cgctcgtccg gtcgcagccc gaggccgccc gcgagctcat
     1621 cgccaagctc tcgaacctgt tgcgcgagcg cctgcagagc cgcgaccagt tcgtcacgct
     1681 tcgcgaggag ctcgacacga tcgatcagta cctggccatc gaggcggtgc ggttcggccc
     1741 tcagctgcgc gtggcgaagc ggatcgagcc cgaggcgctc gacgcgatcg tgcccagcat
     1801 gatcctccag ccgctggtcg agaactcgat caagcacggc ctcaccaaga agatcggcgg
     1861 cggcaccatc tcgctccggg cctttcgcca gaacgggagg tccatcatcg aggtggccga
     1921 cgacggcctg gggatgacgc cggccgatct ggaaaacgcc ctgaccgcgg gcatcggcct
     1981 cagcaacgtc agcgagcgcc tcagggtcac ctacggcgac cactcggcgc tgcagctgac
     2041 ctccgcgcag gggcagggga cctgcgcgcg cctcgagatc cccgagatgg ccgtcgccat
     2101 gaagtcctga ggggcggcaa ggcccattca ggccgcctgt cgcggcggcc tgcagggagg
     2161 ccggcgtgac ggcctcccga ggaggagcag atgcgagcct tggtggtgga cgacgagcag
     2221 ctggcacgcg aggagctgtg cttccagctc gcccgcctcg aggacgtcga ggtggtcggc
     2281 caggctggca acggcgtcga ggccctcgag gtggccgggc ggctgaggcc cgacgtcatc
     2341 ttcctcgaca tccagatgcc gggcctcggc gggttcgagg tcgcccgcga cctcctcgcc
     2401 agcgacaacc ccggccaggt ggttttcgtg accgccttcg accagtacgc catcgaggcc
     2461 ttcgaggtga acgccgtcga ctacctgctc aagccggtcg agccagggcg gctggaggtg
     2521 gccgtccagc gggtgcgccg ccggctgcag ccgtccgccc cattgaacga gcagctcgag
     2581 aagatcgtca gaatgatggg cagggcgccg ggccgccggg gccaggtcgc cgtgaaggtc
     2641 ggcgaccggt tcctgctggt gcaggcagag gacgtgatct acgcgtcggt gaatggagat
     2701 acgataacta tagtgaccgg gcagctgtcg gggacgtcga actaccggac gctcgacgag
     2761 ctgcaggggc ggctcgaccc tgaggtgttt tggcgggtcc accggtcgca cctggtcaac
     2821 atcaacaaga tcaaggagat agtcccctgg ttcagcagga attacatcct caggatgaag
     2881 gacgggaagg gtacggagat cccggtgagc cggtcgcaga cccgccggct gcgggagtac
     2941 ctgaagctct agcccgtccg acgggccccg agccgtggct tccgtgggcc gcctccgacg
     3001 ccgccgcgcc ggcacccgat ccgtcgatag cgctcccggc gcccgcgccc caggcgtcgt
     3061 ccggccgcag tggtgccgtc cgcgctcaac gggcggccat ggtccagcgg gtgcgcgacg
     3121 aactctgggc ctgggtgaag acggtcgcct cggcggccgt ctacgcgacc ctgatcgtga
     3181 ccttcgggtt tcaggtcgcc cgcgtcgagg ggatgagcat ggcgccgacc ctcgaggatc
     3241 aggatcgcct gatcgtcaac aagctcatct atcgtctcag cgcgccgcgc cggggcgaca
     3301 tcgtgatgct gtactacccg gtgaacccgg acaagtcgtt cgtcaagcgg gtcatcgccg
     3361 aggaagggga cttcgtgcgc atcgtggacg ggcgcgtcta cgtgaacgac gtcccgctcc
     3421 ccgacgacta cgtgcccatg gagtaccgca gccacgacga ctggggcccg tcggtgatcc
     3481 aggagggcta ctacttcgtc atgggcgatc accggaacaa cagttccgac agccgccact
     3541 ggggcccggt gccgaagcgg tacatcatcg ggaaggtgca ggtgcggtgg tggccgctgc
     3601 ccgaggccaa gttgttctag ggcctgatcg gccattcgta caggcggtcg atcaatggct
     3661 tccagcgctg ctccaccacc tggcggcgga ccttcatggt cggcgtcagc tctccccgct
     3721 ccatggtgaa ctccgagggc agcaccgcga agcgcttcac gcgctcgaac tgggccagcg
     3781 actcgttcag ccggtccagt atgtcctggt agaggcgctg cacctccggg cgctcggcca
     3841 gctgctcgcc cgcgagcggc ggcaggccga cgcgctcggc ccaggccttg aggcgcggga
     3901 agtcgggcac gatgagcgcc gcggggaact tgcgctgctc gccgatgatc accgcctcgg
     3961 ccaccagtgg gtcggccttc agctggttct cgagcggctg cggcgcgatc ttcttccccc
     4021 ccgaggtcac gatcaggtcc ttcttccgat cggtgatggt gaggtagccg tcggctgaca
     4081 gctggccgat gtcgcccgtg cggagccagc catcaaccag cacctcggcg gtagcctcgg
     4141 ggcgctcgta gtagccctgc atcacgttcg gcccgcgaac gaggatctcc ccatcgggcg
     4201 cgatcgccac ctcgacgccc ggcagcgcct tgccgacggt gcccaggcgc ggcgcgtcgg
     4261 gcgggttgcc cgtgatgccg ggcgacgttt cggtcagccc gtaggcctcg tagatcggca
     4321 ggccgatcgc gaagaagaac tcgccgacct gcttcgagag cggggcgctg cccgacacga
     4381 cgaaccggag ccgccccccc aagcgtgcgc gcatcttgcc gagcacgaga cggtcggccg
     4441 cccggcgctt caaaccgagc gtcgcgggta tggattcgcc ggccagctgg aggcgcgccg
     4501 cctcgtgacc agcctcgagc gcccagtcgg cgagccgccg ccgcggcccg tgcatcttcc
     4561 ccacgacgtc cgcgatggcg gcgtgcactt tctcgaagac ccgcggcacc ccggtcatca
     4621 ccgtcggcct gacctgcacc aggtctcgcg cgaccgtcgt cagcgcctcc gcgaagtaaa
     4681 ccggaacccc gctgtagagg aagcggaaga agaccacccg ctcgaagacg tggctgaggg
     4741 gcaggaacga cagggcgacg tcttcgggct cgagcgtgat ccagccggtc gtggcgagga
     4801 tgttcgacac gatgttcgag tggctgagca tcacgccctt ggggtcgccc gtcgtcccgg
     4861 aggtgtagac gatggtcgca aggtcggagg gggccacctg gcgtgccact tcctcgtagc
     4921 ggcccgcggc gcccgcgtcc gaggccagct gggcgcgtcc ccacgcggtg acgtctggaa
     4981 gagacacccc gcgaacccgc ggcgtcaccg cttccccgtc catcgcgacg atggcctcga
     5041 ggtcgggcag atcgcggacg acctcgttca gcttcgtcac ctgcaccgcg ttcgacacca
     5101 ccgcgatgcg gcagccgctc tccttgagga tgaaccgggc ctggccggcc gacagcgtcg
     5161 ggtagatggg gacggtcacc gcgccggccg agagcgtggc caggtcggtc atcacccact
     5221 cgggccggct gtcgctgatg atggccaccc ggtcgccgcg gcgaaggccg agggcggtca
     5281 acccgagact gagcgcgcgg atcccctcga agaactcctg ggtggaagcc gtggtgcacc
     5341 cgccctcgcg gcagcgcccg accatcgcgg gccgctggaa gcggtcgcga aaatgaaacg
     5401 ggagggtcgc cagggtgaac tggtctgaag aagtcacagg acgcggattc tacaggaaaa
     5461 gcacgacggt ccccaagccg aggtcagtac cctgcttcct tgtccacgac gttcaccagc
     5521 ggttcgccgc gcaggaagcg ctcgaggttg tcggcgaaca ggtccacggt cacctgccag
     5581 tagtcgcgcc ggaag
//
LOCUS       DAOPQX010000237         5587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15900_length_5587_cov_3.900217, whole genome shotgun sequence.
ACCESSION   DAOPQX010000237 DAOPQX010000000
VERSION     DAOPQX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15900_length_5587_cov_3.900217"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKK95_12120"
     CDS             <1..601
                     /locus_tag="PKK95_12120"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC19012.1"
                     /translation="IAQGRNVNILVLDTEVYSNTGGQASKATPIGASAKFAMAGKSRP
                     KKDLGMIAMAYGSVYVAHVAFGAKDAQTVRAFLEADSYDGPSIVIAYSHCIAHGYDLV
                     AGLDQQKLAVDTGYWPLYRFDPRRLERGENPLTMDSAAPKGDIATYMANESRFRVVQQ
                     QDPERYKTLLAAAQKEVAFRYGIYEQLAKLAVPGKPAGE"
     gene            611..1606
                     /locus_tag="PKK95_12125"
     CDS             611..1606
                     /locus_tag="PKK95_12125"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOC19013.1"
                     /translation="MDLTTNYLGLRLPHPVMPGASPLADSLDTVRRLEDAGAAAIVLH
                     SLFEEQITREQLGMVHHMEVHDESFAEAVSYFPKPDEFVFGSEEYCRQIARIKEAVAV
                     PVVASLNGITSEGWLSYAARLQQAGADALELNVYFVATDPAETGAAVEARTVEILRGV
                     KRAVTIPVAVKLSPFYSSLPNLARSLDEAGADGLVLFNRFYQPDIDVEALEATPSLHL
                     SDSSELLLRIRWLAILTGQVRASLAASGGVHTGLDAVKAIMAGASAVQVVSRLLQDGP
                     GQLKTIVGAMRQWLADHEYESLEQARGSMSLRTSPDPSAFERGNYMKILRSWRVA"
     gene            1793..2425
                     /locus_tag="PKK95_12130"
     CDS             1793..2425
                     /locus_tag="PKK95_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19014.1"
                     /translation="MTAKASTSSTTWAVISIACLVLGLAVGYVVFGHRSAPPVVTQVA
                     PQQVPSSGSTPQAGLLDERAVQALRDILARDPKNVQAAIQLGNLYYDAGRYAEAVAPY
                     QQALALDPSNVHVSTDLGTALHYSGRPDEALAQYEKSLAIDPNHAQTLFNQGIVRLDG
                     KQDAPRAIQSWERLLAVNPEYPERARVQQLLEQTRQKMQATPVAPVRSTQ"
     gene            complement(2428..3207)
                     /locus_tag="PKK95_12135"
     CDS             complement(2428..3207)
                     /locus_tag="PKK95_12135"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOC19015.1"
                     /translation="MYPVLFRVGSLEITSFGVMVALGALLGILLLRREAHRSNLDVGR
                     VTDAALVGVLGGLAGAKLLYVLEHWAEPLSDTLLSRGGMSWFGGMTGGIVAGLVAVWW
                     QGLPLMRVLSAAAPSLAVGQAVGRIGCLLVGADYGRPTGLPWGMAFPEGLPPTTDRVH
                     PTQIYEAVVLFALTALLVRFRAKGLRDRAVFASYLIGAGTSRFLIELVRVNVPVLFGL
                     TTAQLFSIAVVAVGIWLVAMGREEPVPAAAAPSGKPRKRRR"
     gene            complement(3239..4231)
                     /locus_tag="PKK95_12140"
     CDS             complement(3239..4231)
                     /locus_tag="PKK95_12140"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOC19016.1"
                     /translation="MGRDLVERHPAGGGPARPVLKWAGGKRQLLPQLRRFYPACFRTY
                     FEPFVGSGAVFFDLHNRGHLAGRQAVLADRNPDLVGCLTAVRDHPEPVIEHLGRLAAE
                     RAADPRAHYYAVRDERFNPLRERLARQAGGLSPERYTPELAAMLIYLNRTGYNGLFRL
                     NGKGSFNVPFGRYANPTICDARNLRRVARALSGCQAGLLHRDFERVLDEAQAGDFVYL
                     DPPYAPLSRTATFTTYTAGGFGEHHQERLQQVVVELARRGCLVLLSNSTAPIISRLYD
                     GNAEAARAGLRACRVPARRAINSDAASRGPVEEYLITNIPESSEGQQASRALSA"
     gene            complement(4233..4508)
                     /locus_tag="PKK95_12145"
     CDS             complement(4233..4508)
                     /locus_tag="PKK95_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOC19017.1"
                     /translation="MTSPTASPGHCHDLLERISRYIDGELPAADRLQMERHLRDCPEC
                     EEVLHSLEHTLDLCREAGRPDLPPELRARAAERVHQLLAQAPERKKR"
     gene            complement(4505..5128)
                     /locus_tag="PKK95_12150"
     CDS             complement(4505..5128)
                     /locus_tag="PKK95_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC19018.1"
                     /translation="MTDSADPNLVHQEPRGAGSPDYAALVRAVQAGDGHAMEELLMRA
                     QEVAYRFSLLVCGRPEEADDAMQEALLKTYRYAGRIREPEAFRAWLYRTVRNACLIGR
                     RKRAGEPAHLLSLHESSGEDGFEPGVDVADPGRDPEAHAMNVSLRGRLFGALRQLPRS
                     YRAVVFLREVEGLSTREVGKVLGISEPNVKTRLHRARVMLRELLERS"
     gene            5172..>5587
                     /locus_tag="PKK95_12155"
     CDS             5172..>5587
                     /locus_tag="PKK95_12155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOC19019.1"
                     /translation="MVTRLTFLPVVLALLVFATGASAQQRLTLDEAISATLQNNPDVR
                     AARAAEREGAARVAEARAAYLPRVDLVEAWQRGNNPVYVFGSLLSQQRFTEANFAVDA
                     LNRPDAVTNHRAAIAVDQPLFDSERLTGIRAAGLGR"
BASE COUNT          870 a         2039 c         1873 g          805 t
ORIGIN      
        1 catcgcccag gggcgcaacg tcaacatcct cgtgctcgac accgaggtct actcgaacac
       61 cggcggccag gcctccaagg ccacgcccat cggggcgtcc gccaagttcg cgatggccgg
      121 caagtcgcgg cccaagaagg acctcggcat gatcgcgatg gcctacggca gcgtctacgt
      181 ggcgcacgtg gccttcggcg ccaaggacgc ccagacggtg cgggcgttcc tcgaggccga
      241 ctcctacgac ggcccgtcga tcgtcatcgc ctacagccac tgcatcgcgc acggctacga
      301 cctggtggcc ggcctcgacc agcagaagct ggcggtggac accggctact ggccgctcta
      361 ccggttcgac ccgcgccggc tcgagcgcgg cgagaatccc ctgacgatgg actcggcagc
      421 cccgaagggg gacatcgcga cctacatggc caacgagtcc cgcttccggg tcgtgcagca
      481 gcaggacccc gagcgttaca agaccctgct tgccgcggcg cagaaggaag tcgccttccg
      541 ctacggcatc tacgagcagc tcgcgaagct ggcggtgccc ggcaagccgg ccggggagta
      601 ggagcctacg atggacctga ccacgaacta tcttggcctt cgcctgccgc acccggtgat
      661 gcccggcgcg tcgccgctcg cggattccct ggacacggtg cgccggctcg aggacgccgg
      721 cgccgccgcc atcgtcctcc actcgctgtt cgaggagcag atcacccggg agcagctcgg
      781 catggtgcac cacatggagg tgcacgacga gtcgttcgcc gaggcggtct cctacttccc
      841 gaagccggac gagttcgtct tcggatcgga ggagtactgc cgccagatcg cccggatcaa
      901 ggaggcggtg gccgtccccg tcgtcgcctc cctcaacggc atcaccagcg aggggtggct
      961 ctcctacgcc gccagactgc agcaggcagg cgccgacgcc ctcgagctga acgtctactt
     1021 cgtcgccacc gacccggccg agaccggcgc ggccgtcgag gcgcggacgg tggagatcct
     1081 gcgcggcgtc aagcgggcgg tgaccatccc ggtggccgtc aagctgtccc ccttctactc
     1141 ctcgctgccg aacctggcgc gctcgctcga cgaggcgggc gccgacgggc tcgttctgtt
     1201 caaccggttc taccagcccg acatcgacgt cgaggcgctc gaggccacgc ccagcctgca
     1261 cctctccgac tcctccgagc tgctgctccg catccgctgg ctggcgatcc tcaccggcca
     1321 ggtgcgggcc tcgctggccg cctcgggcgg tgtccacacc ggcctcgacg cggtgaaggc
     1381 catcatggcg ggcgcctcgg ccgtgcaggt cgtctcgcgc ctgctgcagg acgggcccgg
     1441 gcagttgaag acgatcgtgg gcgcgatgcg tcagtggctc gcggaccacg aatacgaatc
     1501 gctcgagcag gcccgcggca gcatgagcct gcgcacgagt cccgacccgt cggccttcga
     1561 gcgcgggaac tacatgaaga tcctgcgcag ctggagggtg gcgtagggga gcggctcagg
     1621 gctcacggct cacggctcaa ggccctgggc tttccggtag actagatcca ggtcggggcg
     1681 acgcacgatg cgccgccccg acttgtctca ggacctcgcg gcctcgacct agacttcgtg
     1741 acttcgtgga ctccgacttc gtgacttcgc gacttcgcga cttcgtggac ctatgactgc
     1801 caaagcctcc acctcctcca ccacctgggc cgtcatctcg attgcctgcc tcgtcctcgg
     1861 gctcgcggtc ggctacgtcg tgttcggcca ccgctccgcc ccgccggtcg tcacccaggt
     1921 cgcaccgcag caggttccct cgtccggcag cacgccgcag gctggcctgc tcgacgaacg
     1981 agcggtgcag gcgcttcgcg acatcctcgc gcgcgacccg aagaacgttc aggcagccat
     2041 ccagctgggc aacctttact acgacgccgg ccgctatgcc gaggcggtcg caccgtacca
     2101 gcaggcgctg gcgctcgacc cctccaacgt gcacgtcagc accgacctcg gcaccgcgct
     2161 ccattactcc ggccgcccgg acgaggcgct ggcccagtac gagaagtcgc tcgccatcga
     2221 ccccaaccac gcgcagacgc tgttcaacca gggaatcgtg agactggacg gcaagcagga
     2281 cgcgccgcgc gccatccaga gttgggagag gctgctggcc gtcaacccag agtacccgga
     2341 gcgcgcccgg gtgcagcaac tgctcgagca gacccgccag aagatgcagg cgaccccggt
     2401 cgctccggtc cgatcgacac agtaaggcta ccgccggcgt ttgcggggct tgccggaagg
     2461 cgccgccgcg gccgggaccg gctcctccct ccccatcgcc accagccaga ttcccaccgc
     2521 gacgaccgcg atcgagaaca gctgcgcggt ggtgaggccg aagagcaccg gcacgttcac
     2581 tcgcaccagc tcaatcagga accggctcgt cccggccccg atcaagtacg acgcgaacac
     2641 tgcccggtcg cgaagaccct tggcgcggaa gcggaccagc agcgccgtca gcgcgaagag
     2701 gacgacggct tcgtagattt gcgtcggatg gacgcggtcg gtggtcggcg gcagcccttc
     2761 cggaaacgcc atcccccagg gaaggccggt cgggcgtccg tagtcggctc ccaccagcag
     2821 gcagccgatc cggcccacgg cctgcccgac cgccagcgac ggcgccgccg cgctgaggac
     2881 gcgcatcagc ggcagcccct gccaccagac ggccacgagg cccgccacga tgcctcctgt
     2941 catgccgccg aaccagctca tgcctccgcg gctcagcagc gtgtccgaca gcggctcggc
     3001 ccagtgctcc aggacgtaga gcagcttggc gcccgccagc ccacccagta cccccaccag
     3061 agccgcgtcg gtgacccggc cgacgtccag gttcgagcga tgcgcttccc ggcgcagcag
     3121 caggatcccg agcagcgccc ccagggcgac catcacgccg aagctggtga tctcgagcga
     3181 accgacgcgg aaaaggacgg ggtacatggg acagatgata gtccatccat cgcaccgatt
     3241 acgctgaaag ggcccggctc gcctgctgcc cttccgagct ctcggggatg ttcgtgatca
     3301 ggtactcctc gaccgggccc cgcgacgccg cgtcggagtt gatcgcccgg cgggccggca
     3361 cgcggcaggc gcggagcccc gcacgggcgg cctctgcgtt cccgtcgtag agccgcgaga
     3421 tgatcggggc cgtggagttg ctcaagagga ccaggcagcc gcggcgggcc agctcgacga
     3481 ccacctgctg cagccgctcc tggtggtgtt cgccgaaccc gccggcggtg tacgtggtga
     3541 aggtggccgt ccggctgagg ggcgcgtacg gaggatcgag gtagacgaag tcgcccgcct
     3601 gcgcctcgtc gagaacccgc tcgaagtcgc ggtgaaggag cccggcctgg cagcccgaca
     3661 gcgccctcgc cacgcgacgc aggttgcggg cgtcgcagat cgtggggttc gcgtaccggc
     3721 cgaacggcac gttgaaggag cccttgccgt tcaggcggaa cagcccgttg tatcccgtgc
     3781 ggttcaagta gatcagcatc gccgccagct cgggcgtgta ccgctcgggc gacagtccgc
     3841 ccgcctgacg ggcgagccgt tcacgcagcg ggttgaaccg ctcgtcgcgg accgcgtagt
     3901 agtgggcgcg cgggtcggcg gcccgttcgg cggccagcct gcccaggtgc tcgatgaccg
     3961 gctccgggtg gtcgcgaacc gcggtcaggc acccgaccag gtcggggttg cggtcggcga
     4021 gcaccgcctg gcggcccgcg aggtgcccgc ggttgtgcag gtcgaagaac accgcaccgc
     4081 tgccgacgaa gggctcgaag taggtacgaa aacacgccgg atagaaacgt cggagctgcg
     4141 gcaggagctg gcgcttgcca cccgcccact tcaggaccgg gcgcgcggga ccgccgccgg
     4201 cggggtgtcg ttcgaccagg tctcgaccca cgctacctct tcttccgctc cggggcctgt
     4261 gccagcagct ggtgcacgcg ctcggcggcg cgggcgcgaa gctccggggg caggtcgggg
     4321 cgccccgcct cgcggcacag gtccagggtg tgctccaggc tgtggagtac ctcttcgcat
     4381 tcggggcagt cgcgcaggtg gcgttccatc tgcaggcggt ccgccgccgg cagctcgccg
     4441 tcgatgtatc gggagatccg ctcgagcagg tcgtggcagt gtccaggcga cgcggttggg
     4501 ctcgtcatga ccgttccagt agttcgcgca gcatcacgcg cgcccggtga agccgcgtct
     4561 tgacgttcgg ctcggagatg cccaggacct tccccacctc tcgagtggag agcccctcga
     4621 cctcccgcag gaagaccacc gcgcgatacg agcggggcag ctggcgcagc gcgccgaaca
     4681 accgccccct gagcgacacg ttcatggcgt gcgcctccgg gtcgcgtccc gggtcggcca
     4741 catcgacccc cggttcgaag ccgtcctcgc cgctgctctc gtgcagcgac agcaggtggg
     4801 ccggctcgcc cgcgcgcttg cgccggccga tgaggcacgc gtttcggacg gtgcggtaca
     4861 gccacgcgcg gaaggcctcc ggctcgcgga ttcgtcccgc gtaccggtag gtcttcagca
     4921 gcgcctcctg catcgcatcg tcagcctcct ccgggcggcc gcagaccagc aggctgaagc
     4981 ggtacgccac ctcctgcgcc cgcatcagga gttcctccat cgcgtggcca tccccggcct
     5041 gcaccgcccg gacgagtgcg gcgtagtcgg gagaaccggc cccgcggggc tcctggtgaa
     5101 caagattcgg atcggctgaa tcggtcacac tgtgacctct cgacggcttc ctgaatcacc
     5161 tagcagagcc tatggtaacg cgactgacgt tcctgccggt cgtgctggcc ctgctggtct
     5221 tcgcgacggg cgcttccgcc cagcagcgcc tcacgctcga cgaggcgatc tcggccacgc
     5281 tgcagaacaa tcccgacgtg cgggccgccc gcgccgccga gcgcgagggc gcggcgcgtg
     5341 tcgccgaagc cagggcggcg tacttgccgc gggtcgacct ggtcgaggcg tggcaacgcg
     5401 gcaacaatcc cgtctacgtc ttcgggtcgc tgctgtcgca gcagcggttc accgaggcca
     5461 atttcgccgt cgacgccctc aaccggccag acgccgtcac caaccaccgg gccgcgatcg
     5521 ccgtcgatca gccgctgttc gactccgagc gcctgaccgg gatccgcgcc gcgggcctgg
     5581 ggcgcca
//
LOCUS       DAOPQX010000238         5576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15941_length_5576_cov_4.165006, whole genome shotgun sequence.
ACCESSION   DAOPQX010000238 DAOPQX010000000
VERSION     DAOPQX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15941_length_5576_cov_4.165006"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..2299)
                     /locus_tag="PKK95_12160"
     CDS             complement(62..2299)
                     /locus_tag="PKK95_12160"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOC19020.1"
                     /translation="MHHRHVRRLLAPALAAACVLSASASTPIFWQVSTRAEFMKGEVE
                     DLAIDNDGRLSLGPASALVHDAASPFVWALVAAPDGSVYAGTGNDGKVFRVAPDGKAS
                     PLFDAPELEVHAVAPAKDGTLYAATSPDGRIYKVDGTGQATPFYDPEDKYIWSLAIGP
                     DGYVYAATGERGTIYRIAPDGKGDVFYRTKATNVVSLAFDTAGNLLASTEAPGRVFRI
                     DPKGKGFVLLDSPYREVRSLRLREDGSIYAAAVAGSEPAAAPAGEAAITPPQPARSAP
                     VPTVTTEVTSITVIDVGAPETSKPARVTPRVAKGAVYRIGPDGVWDVIWESQEDTPYD
                     VAFEDKNAVLIATGAKGKIYRVTGDPPQVSLVARAGGQQVTRFLQTARGETWYATSNP
                     GKIFRFSSARAESGWFESEVRDATTVSSWGTISWRARVPEGARVELRTRSGNSATPDE
                     TWSPWSEAYAEPGGEQIRSPKARYLQWKATLAGKGESPVLTSVTTAYLQRNLRPRVTG
                     ITVYPPGIVFQKPFSTGETEIAGFEEGWPDTRPSPAALAAGTPTPSSSQGPALGRRTY
                     QKGLQAFTWKAEDDNDDNLQYDVLYRRETDVAWKTVRRAITDQLFVWDTTSVPNGTYI
                     LRIVATDAPSNPPGAALAGEADSVSFDVDNTPPSIQVTGVRRQGDANVIAFEVRDDHA
                     AVQRVDFSNDGTRWRPIYPVDGICDSRVERFELTVDGDPANVVIRAMDAMSNVSTARA
                     NGTPK"
     gene            complement(2310..4157)
                     /locus_tag="PKK95_12165"
     CDS             complement(2310..4157)
                     /locus_tag="PKK95_12165"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOC19021.1"
                     /translation="MNSRILCSVVFLGLLTAVWPSAATRYMSADEVRPGMVGIGRTVF
                     EGNRIEEFKVHLLGVLRNTTGPRRDLVLARLEGGPLANTGVIAGMSGSPVYVGDRLLG
                     AVAYSLGQFSKEPIAGITPIAEMVETADPAARRGPGEKTRISLPLTRESMAAALQRAF
                     ARFERPFAEGGPVLTLTGSQAVRPDIGPLLRPIATPLALGGFTGPVGETLAGAFRDSG
                     FVAVPGAQAQPEDKAPRAGGSSTLRPGDAVGVNLVTGDFSVGATGTVTEVDGDKVYAF
                     GHPLYNLGPTEFPMTRAYVYTLLPSLATSTKVATTGETIGTFKQDRSTAIAGTLGAGP
                     TLVPVNIALETARGLKKTFRFEVVQDQMFTPLLTYVSILNVLSSYEREYGAATFRVKG
                     KAMVRRHGTVAFEDVFTGESPSIGAAAYVVGPLNFLLSNDFEPVHLEGVDLAITTSEQ
                     PETVRLERAWLDVPRVRAGTTVPLRLQLRTYRGDEVLRTVPVDIPANAKGSLSILITD
                     GSSLAQIEQRESRGVTQVQDLAQMIRVLNDTKKNNRFYVRLLSQDPGAVVNGEALPSL
                     PPSVLSVLEGDRSGGSFSPLRNATLREWEVATDSAVSGTRILTVTLDSN"
     gene            4263..>5576
                     /locus_tag="PKK95_12170"
     CDS             4263..>5576
                     /locus_tag="PKK95_12170"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOC19022.1"
                     /translation="MPRTQVCATVAAAGIDALRRARDAASRVADLVELRIDHLDRPDV
                     RGALEGRSGRVLVTCRPAWEGGGFAGAEDERLGLLEQALDLGAEYVDVEWRAGDRARD
                     LVRRRQGRGIVLSLHDYDGIPVDLAGVWRSMAASGAEVVKLAVTPRSLADTLRLLTLG
                     REAAGLARVALVGMGPAGLCTRILASRFHSCWTYAGNAVAAGQLPLSTMLELYGFRSI
                     GDGTAVYGIVGRPIGHSVSPWIHNAAFQARGIDAVYVPFEAESIEDFRRLALALDLRG
                     ASVTAPFKEDVVPYLTSADGLTRQSGAANTIRVEGDRWMGRNTDVEGFLRPLRGRVRL
                     DNLRVAIVGAGGAARAVAAALSNTGAVVSVHARRAEAAAAVARITAGRVGERGPRAGE
                     WDLLVNATPVGTSPGVEALPVDPACLRGGIVYDLVYNPPMTALRRE"
BASE COUNT          765 a         1920 c         2030 g          861 t
ORIGIN      
        1 cgctccgcct tgcgcgccgc cggcctctcg gcctagctgg gcgcaccgct ctctcactcc
       61 ttcacttcgg cgtcccgttg gcgcgcgcgg tcgagacgtt gctcatcgcg tccatggcgc
      121 ggatgacgac gttggccggg tcgccgtcca ccgtcagctc gaatcgctcc acgcgcgagt
      181 cgcagatgcc atcgacgggg tagatggggc gccagcgtgt gccgtcgttc gagaagtcca
      241 ctcgctggac ggccgcatgg tcgtcgcgca cctcgaaggc aatcacgttc gcatccccct
      301 ggcgacgcac accggtcacc tggatcgacg gcggcgtgtt gtcgacgtcg aagctgacgc
      361 tgtcggcctc gccagccagg gccgcgccgg gagggttcga cggcgcgtcg gtggcaacga
      421 tccgcaggat gtacgtgccg ttgggcaccg aggtcgtgtc ccagacgaag agctgatcgg
      481 tgatggcgcg gcgaaccgtc ttccaggcca cgtcggtctc gcgccggtac agcacgtcgt
      541 actgcaggtt gtcgtcgttg tcgtcctccg ccttccaggt gaacgcctgc agccccttct
      601 gatacgtacg gcggccgagc gcggggccct gggaggagga aggcgtgggc gtccccgccg
      661 ccagtgcggc gggcgagggc cgcgtgtcgg gccaaccctc ctcgaagccg gcaatctcgg
      721 tctcacccgt cgagaacggc ttctggaaga cgatgcccgg ggggtagacg gtgatgcccg
      781 tgacgcgcgg gcggaggttg cgctgcaggt aggcggtggt gaccgaggtg agaaccgggc
      841 tctcgccctt gccggccagg gtggccttcc actgcaggta gcgggccttc gggcttcgaa
      901 tctgctctcc gcctggctcg gcgtacgcct cggaccaggg gctccaggtc tcgtcgggag
      961 tagcgctgtt gcccgaccgg gtgcgcagct cgacgcgcgc cccctcggga accctggccc
     1021 gccagctgat cgtcccccag ctcgagaccg tggtcgcgtc gcgcacctcc gactcgaacc
     1081 agcccgactc ggctcgcgcc gacgagaacc ggaagatctt ccccggattc gaggtggcgt
     1141 accaggtctc gccgcgggcc gtctggagaa agcgcgtgac ctgctgcccg ccggcgcgcg
     1201 ccacgagcga cacctggggc gggtccccgg tcacgcgata gatcttcccc tttgcgccgg
     1261 tcgcgatgag caccgcgttc ttgtcctcga aggcgacatc gtacggcgtg tcctcctgcg
     1321 attcccagat cacgtcccag acgccgtcgg gaccgatgcg gtacacggcg cccttggcga
     1381 cgcgcggcgt gacgcgcgcc ggcttggagg tttcaggtgc gcccacgtcg atgacggtga
     1441 tcgaggtcac ctcggtcgtc acggtcggga ccggcgccga ccgggctggc tggggaggcg
     1501 tgatcgccgc ttcgccggcc ggggcggccg ccggctcgct cccggccaca gccgcggcgt
     1561 agatcgaccc gtcctcgcgg aggcgcagcg accggacctc gcggtacggc gagtcgagga
     1621 gcacgaaacc cttgcccttg ggatcgatcc tgaacacacg accgggggcc tcggtcgacg
     1681 cgaggaggtt gccggcggtg tcgaacgcga ggctcaccac gttggtcgcc ttggtccggt
     1741 agaagacgtc gcccttgccg tctggggcga tccggtagat cgtgccgcgc tcgccggtcg
     1801 cggcgtagac gtagccgtcg gggccgatgg ccagcgacca gatgtactta tcctcggggt
     1861 cgtagaaggg cgtcgcctgg ccggtgccgt ccaccttgta gatccgcccg tccggcgagg
     1921 tggcggcgta cagcgtgccg tctttcgccg gggccacggc gtgcacctcc agttcgggcg
     1981 cgtcgaagag gggggaggcc ttcccgtcgg gcgcgaccct gaagaccttg ccgtcgttgc
     2041 cggtccctgc gtagacgctg ccatccggtg cggcgaccag cgcccacacg aacggcgagg
     2101 ccgcgtcgtg gacgagggcg ctcgccgggc cgagcgagag gcgcccgtcg ttgtcgatgg
     2161 caaggtcttc cacctcgccc ttcatgaact cggcgcgcgt ggagacctgc cagaagattg
     2221 gcgtggaggc ggacgctgag agaacgcagg cggccgcgag cgcgggcgcc agcaggcggc
     2281 ggacgtgtcg gtggtgcatg agtgagccgt cagttcgagt ccagtgtgac ggtgaggatc
     2341 cgcgtgccgg agaccgcgct gtcggtcgcg acctcccact cccgcaaggt ggcgttccgg
     2401 agcggcgaga agctgccccc gctgcggtcg ccctcgagga cagacagcac ggagggcggc
     2461 agcgagggca gcgcctcgcc gttgaccacc gcgcccgggt cctggctgag caggcggacg
     2521 tagaagcggt tgttcttctt ggtgtcgttc aggacgcgga tcatctgagc gaggtcctgc
     2581 acttgcgtca cgccccggct ctcgcgctgc tcgatctgcg cgaggctgct cccgtcggtg
     2641 atcaggatcg acagcgagcc cttcgcgttg gcgggaatgt ccaccggaac ggtgcgcagt
     2701 acctcgtcgc cccggtacgt gcgaagctgg agccgcagcg ggacggtcgt gccggcgcgc
     2761 acgcgcggga cgtcgagcca cgccctctcg agccgaaccg tctcgggctg ctcgctggtg
     2821 gtgatggcca ggtctacccc ctcgaggtga acgggctcga aatcgttcga gaggaggaag
     2881 ttcagcggcc cgacgacgta tgccgccgcg ccgatggacg gcgactctcc ggtgaagacg
     2941 tcctcgaacg ccaccgtgcc gtgccggcgc accatcgcct tgcccttgac ccggaaagtc
     3001 gccgcgccgt actcgcgctc gtacgagctc agcacgttca ggatcgacac gtaggtgagc
     3061 aggggggtga acatctggtc ctgcaccacc tcgaaccgaa aggtcttctt gaggcccctc
     3121 gccgtctcga gggcgatgtt caccgggacg agggtcgggc cggcgccgag ggttcccgcg
     3181 atcgccgtgg atcggtcctg cttgaaggtg ccgatcgtct cgcccgtggt ggcgaccttc
     3241 gtcgaggtcg cgaggctcgg caggagcgtg tacacgtagg cgcgcgtcat cgggaactcg
     3301 gtcggcccga gattgtagag ggggtgcccg aaggcgtaga ccttgtcccc gtccacctcg
     3361 gtgaccgtcc cggtcgcccc caccgagaag tcgccggtga ccaggttgac cccgaccgcg
     3421 tcgccgggac ggagcgtgct gctcccaccg gcgcgaggcg ccttgtcctc gggctgtgcc
     3481 tgggcgccgg gaacggcgac gaagccggag tcgcggaacg cgccggccag cgtctcgccg
     3541 accggaccgg tgaagccgcc gagggccagc ggggtggcga tcgggcggag gagcgggccg
     3601 atgtccggcc gcaccgcctg gctgccggtc aaggtcagca cgggcccgcc ctcggcgaac
     3661 ggccgctcga agcgggcgaa cgcgcgctgc agcgccgccg ccatcgactc gcgcgtgagc
     3721 ggtagcgaga tccgggtttt ctcgccgggg ccgcggcgcg ccgccgggtc tgccgtctcc
     3781 accatctcgg cgatcggcgt gatgcccgcg atcggttcct tggagaactg gccgagcgag
     3841 taggccaccg cgcccagcag ccggtcgcca acgtagacgg ggctgccgct catgcccgcg
     3901 atgacgcccg tgttggccag cggcccgccc tcgaggcggg ccagcaccag gtcgcggcgc
     3961 ggacccgtgg tgttccggag caccccgagc aggtgaacct tgaactcctc gatgcggttg
     4021 ccctcgaaaa cggtgcggcc gatcccgacc atgccgggac ggacctcgtc ggcggacatg
     4081 tagcgtgttg ccgccgacgg ccagacggcc gtcagcaggc cgaggaagac gaccgagcag
     4141 agaatccgag agttcatgaa cgaactgggg tccttcgcgt atggcgtgat gacgggccgg
     4201 atcgccttca ttatacgctg ccttgacgcg cggcaccggc tctgcaaccc ttgaggcggc
     4261 ccatgccacg cacgcaagtc tgcgccacgg tggccgctgc cggcatcgat gcgttgaggc
     4321 gggcccgcga tgccgcgtcg cgggtcgccg acctcgtcga gctgcgcatc gaccacctcg
     4381 accgccccga cgtccgcggc gccctggagg gccgatcggg tcgcgtcctg gtcacctgcc
     4441 gcccggcctg ggaagggggc ggcttcgccg gggccgagga cgagcgcctc ggcctgctcg
     4501 agcaggccct cgacctcggc gccgagtacg tggacgtcga gtggcgtgcc ggcgaccgtg
     4561 cgcgcgacct cgtccgccgc cggcaggggc gcgggatcgt gctctcgctt catgattacg
     4621 acggaattcc ggtcgacctg gccggtgtgt ggcggtcgat ggccgccagc ggggccgagg
     4681 tggtgaagct ggccgtgacc ccgcgcagtc tcgccgacac cctccggctg ctgacgctcg
     4741 gccgcgaggc cgcagggctg gcccgggtgg cgctggtggg aatgggcccg gccggcctgt
     4801 gcacacgcat cctggcctcg cggttccact cgtgctggac ctacgcgggc aacgccgtcg
     4861 ccgcagggca gttgcccttg tccacgatgc tcgagttgta cggcttccga tcgattggtg
     4921 acggaaccgc cgtctacggc atcgtcggcc gcccgatcgg acactcggtg tcgccgtgga
     4981 ttcacaacgc cgcctttcag gcccgcggca tcgacgccgt gtacgttccg ttcgaagccg
     5041 aaagcatcga agacttcaga cgattggcgc tcgcgctcga cctgcggggg gccagcgtca
     5101 cggccccgtt caaggaagac gtcgtgccct acctcacgag cgcggacggc ctgacgcggc
     5161 agtctggagc ggcaaacacc atccgggtcg agggcgaccg ctggatgggc cggaacaccg
     5221 acgtcgaggg cttcctgcgc ccgctccggg gacgcgttcg cctcgacaat ctccgcgtag
     5281 ccatcgtcgg cgcgggcggg gccgctcgcg ccgtggcggc cgctctctcc aacaccgggg
     5341 cggtggtctc cgtccacgcg cgccgcgccg aggcggcggc cgcggtggcc aggatcacgg
     5401 ccgggagggt gggggagcga gggccgcggg cgggcgaatg ggacctgctc gtgaacgcga
     5461 cgccggtcgg cacgagtccc ggggtcgagg ccctcccggt cgatcccgcc tgcctgcggg
     5521 gtgggattgt ctacgatctc gtctacaatc cgccgatgac cgcgctgcgc cgcgag
//
LOCUS       DAOPQX010000239         5567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15972_length_5567_cov_4.148585, whole genome shotgun sequence.
ACCESSION   DAOPQX010000239 DAOPQX010000000
VERSION     DAOPQX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15972_length_5567_cov_4.148585"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="PKK95_12175"
     CDS             <1..1502
                     /locus_tag="PKK95_12175"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOC19023.1"
                     /translation="RYHDKPSVRGTGPGPFPWPGPALDGYGPFLERRDMEAAAPRGLP
                     AAGEPREDDRAGQLEAALGHLERLFGRLERAEQQFKRLTDECEGVLHGLVAVDRRHSA
                     AIASLNERLGDWCNLEGKLLEESARRIERFERGVSHEWMVLRRLNEEPIAELREQADA
                     LRRTCLEAARIARLRLEGAEQARASHAEDIDRRLAEWTRQLLQAAQRQGSDAAALGTP
                     RPAAGVEPWSFEGVAQLHQELRGGAPRPEAAETGGNGAGRDDREPAVAAPRSSRSQAG
                     HAGTDERLALDQPQEQPGEQPLEEEPRSGSLGRRTWFVALGGVALAALLVVLVLGTGG
                     PGTPNAASPPSREAAERQAAEAPPPPPAVPEPDPRVLEAQRAAERAAVMVEILAAPDL
                     RRYALGGLRATPAAYAQVLWSRTRGLAVTASRLPAPPPNTAYQVWIRNAAGAASAGLL
                     TPDANGRASLVLAGPLKLPQPGAIRVTIEPQAGSPHPTGELCLAGGPSE"
     gene            1499..3676
                     /locus_tag="PKK95_12180"
     CDS             1499..3676
                     /locus_tag="PKK95_12180"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC19024.1"
                     /translation="MSVEPAAHRVLNRRRFTAEEVHRPPGDSAASRALFAWYAPGFSD
                     RLGDRLNLFDNTDGPPLELLRLRAELSRAADFEFSLRARVSELADFQHPRFARVFRAD
                     RLPEPGGGLALVSARTDGLRLSDVLRLADQRAVRLSANLLLPLVADATDAVAALHARG
                     PHVAHLALCADRLIVAPGGRLQVAEYVLGSALRGLRYSRAEMWRLLRVALPSGAAPNL
                     DQRVDVFQLGLVGLSLALARPLDDDEDLDRIRGLLGEADRRVPGAGWNGLWPAFREWL
                     CRALQLDARTFTDAWDAGAALAEAVPLDVRRPSPMQGAAAWDQFVSDCEAPPILTGRD
                     KGSRPALSAGGGPGGAQPVQPTRNPAPVTGLDDWERPSTPPSAESESLPAGRPEEVHG
                     PPLPPSPPRRPLRRETTRAPRLVTPAVVAALAIATMGGGVAIGARFFAPGRQAADSSV
                     HVDSSPQGALVTVDGAPAGRTPVTLSLSPGSHLLELVAGSSRRTVPVSLAKGARLSHF
                     VEMPAASTSGELRVTTDPQGLRVIVDGKLRGTSPLLVSDLAPGPHVVAVEGRGRSARH
                     EVKVDAGATAALFVPLSSGGAPSSGWLSIAGEIELQVYENGELVGTSRSPRIMLPAGL
                     HEVELVNEELGVRIGEEVRISPGGTSSLEVDLPQGRMDVNALPWAEVWLDGKRIGETP
                     LGGVPARVGRHMLTFRHPQLGERRVECLVAAGRPTRVGVDLRK"
     gene            3685..4446
                     /locus_tag="PKK95_12185"
     CDS             3685..4446
                     /locus_tag="PKK95_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC19025.1"
                     /translation="MAYQLLVMDDSVTIRRVVELTFAGGDVQVVGVGDGRAALERIAA
                     EPPDVILADVGTSQPDGYEVSAYVKGNPALAHIPVLLLSGAFQPIDEERARAAGCDGV
                     LAKPFDPGAVAARVRQVLEERGAKPEPSPPSDEMIAARSADPAAAPDEDADLLAFFEE
                     LLAQPLLEGSDDACREEAACRPEPCAPAPLPAPGGQSPFSLDDYFDLLDEILAGGLLR
                     SGPATSPSPSPGGPPRPPRSSSRACSGCPRGSTAS"
     gene            complement(4349..>5567)
                     /locus_tag="PKK95_12190"
     CDS             complement(4349..>5567)
                     /locus_tag="PKK95_12190"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOC19026.1"
                     /translation="IQGLNNGLTNTKGVLEGLVKGGSLERKLQAERALADWIAADPGR
                     RQQYGEVLPAMQALHEASLKTRERNAALAGLSGASVYLNAAQTLYRLSLQRPKRDIDR
                     DQGFQERDWSRLRAAQERLQRTIDPTVDRALLSWAMGKAASLPEGQRIEPLDTLAGLR
                     PGRSQAEVSQAIDTYLNRLYAGTRLADQDVRLALLEKSAAELKASNDPFIELAAALEP
                     LNESIREAGKDLAGAYAKLRPRYMEAMLARAGGLVAPDANSTLRVTFGQVKGVRARDG
                     LFYEPQTTLEGIVEKHTGEGEFNAPKAQLHAIEALRGGAKTPYLVPELGDVPVNFLST
                     VDTTGGNSGSPTLNARGELVGLLFDGTYESVASDYLFEPVKTRSIHVDSRYMLWTMSE
                     VDGAGHLVKEMGR"
BASE COUNT          681 a         2118 c         1988 g          780 t
ORIGIN      
        1 ggcggtacca cgacaagccg agcgtgcggg gaacggggcc tggcccgttt ccttggccgg
       61 gtcctgcact ggacgggtac ggcccgttcc tggagagacg cgacatggag gcagcggcgc
      121 cccggggact gcccgcggcc ggagagccgc gagaggacga tcgagccggg cagctcgagg
      181 cggcgctcgg acacctcgag cgccttttcg ggcgcctcga acgggccgag cagcagttca
      241 agcggctcac cgacgagtgc gagggggtgc tgcacgggct cgtggcggtg gatcgccgac
      301 actcggcagc catcgccagc ctcaacgagc ggctcggcga ctggtgcaat ctcgagggca
      361 agctgctcga ggagtcggcg cggcgcatcg agcgcttcga gcgcggcgtc agccacgagt
      421 ggatggtgct gcggcgcctg aacgaggagc cgatcgccga gttgcgggag caagccgacg
      481 ccctgcgccg cacctgcctc gaggccgccc gcatcgcccg gctgcgcctc gaaggcgcgg
      541 agcaggcgcg cgcctcgcac gccgaggaca tcgaccgtcg cctcgcggag tggacgcgtc
      601 agctcctgca ggcggcgcag cgccagggga gcgacgccgc ggcccttggc acgcctcgcc
      661 ccgctgccgg cgtcgagccc tggtcgttcg agggagtcgc gcagctgcac caggagctgc
      721 gcggtggggc gccgcgcccg gaagccgccg agaccggcgg caacggcgcc ggtcgcgacg
      781 accgggagcc ggccgtcgca gccccacgat cgagccggtc ccaagcaggt cacgccggca
      841 ccgacgagcg gctggcactc gaccagccgc aggagcagcc cggcgaacag ccgctggagg
      901 aggagccgcg ttccggcagc ctcgggcgcc gaacgtggtt tgtcgcgctt ggcggcgtgg
      961 cgctcgccgc gctcctcgtc gtcctcgtgc tggggaccgg cgggccggga acccccaacg
     1021 ccgcctctcc accctcgcgg gaagccgccg aacggcaagc cgccgaagcg ccgcccccgc
     1081 cgcccgcggt tcccgagccc gacccccggg tgctcgaggc gcagcgtgcc gcggaacggg
     1141 cggcggtgat ggtggagatc ctcgccgcgc ccgacctgcg ccgctacgcc ctgggaggcc
     1201 tgcgcgccac gcccgccgcc tacgcgcagg tgttgtggag ccgcacgcgc gggctggccg
     1261 tcactgcgtc acgcctgccc gccccgccac ccaacaccgc ctaccaggtg tggatccgga
     1321 acgccgcggg ggccgcatcg gccggcctgc tcacgcccga cgcgaacggg cgcgccagcc
     1381 tcgtgctcgc cgggcccctg aaactgccgc agccgggcgc catccgcgtc accatcgagc
     1441 cgcaggccgg ctcgccccat ccaaccggag agctgtgcct ggcgggagga ccgagcgaat
     1501 gagcgtcgag ccggccgctc accgcgtgct gaaccggcgc cgcttcaccg ccgaggaggt
     1561 gcaccggccc cccggcgact cggccgcctc gcgagccctg ttcgcgtggt atgcgccggg
     1621 cttctcggat cggctgggcg atcgcctcaa cctcttcgac aacaccgacg gtccgccgct
     1681 cgagttgctg cgcctccgcg ccgagctgtc gcgcgccgcc gacttcgagt tctctctgcg
     1741 cgcgcgggtg agcgagctcg ccgacttcca gcatccgcgc ttcgcccgcg tcttccgggc
     1801 cgatcgcctg cccgagccgg gcggcggcct tgcgctcgtc tccgcccgca ccgacggcct
     1861 caggctctcc gacgtgcttc ggctcgccga ccagcgcgcc gtgcggctct cggccaacct
     1921 cctgctcccg ctcgtcgccg acgcgaccga cgcggttgct gcgctgcatg cccgcgggcc
     1981 gcacgtcgcc catctcgccc tctgcgccga ccggctgatc gtcgcgcccg gcggacggct
     2041 tcaggtcgcg gagtacgtgc tcggctcggc gctgcggggg ctgcgttact cgcgtgcgga
     2101 gatgtggcgg ctgctgcgcg tggcactgcc ctccggggcc gccccgaatc tcgaccagcg
     2161 cgtcgacgtc ttccagctgg gcctcgtcgg cttgtcgctc gccctggccc gccctctcga
     2221 cgacgacgag gatctcgacc ggatacgggg gctcctcggc gaggccgacc gcagggtccc
     2281 gggcgcgggc tggaacggcc tgtggccggc cttccgcgag tggctctgcc gggcgctgca
     2341 gctcgacgcc cgcacgttca ccgacgcgtg ggacgctggt gcggcactcg cggaggcggt
     2401 gccgctcgac gtgcggcggc cttcgccgat gcagggagcg gccgcctggg accagttcgt
     2461 ctccgactgc gaggcgccgc cgatcctcac gggccgcgac aagggttcgc gcccggcgct
     2521 ctccgccgga ggcggtcccg gaggcgctca gcctgtccag cccacccgca accctgcccc
     2581 ggtcaccggc ctcgatgact gggagcgccc atccactccg ccctccgcgg aatcggagag
     2641 tctgccggct gggcgccccg aggaggtgca cgggccgccg cttcctccct cgcccccgcg
     2701 aaggcccttg cgccgcgaaa ccacccgcgc cccccgcctc gtcaccccgg ccgtcgtcgc
     2761 cgcgctggcg atcgcgacga tgggaggcgg cgtcgcgatc ggggcgcgct tcttcgcccc
     2821 gggccgccag gcagccgaca gctcggtcca cgtcgactcg agcccgcagg gggcgctggt
     2881 aaccgtggac ggtgcgcccg cggggcggac acccgtcacg ctgtcgctct cgcccggctc
     2941 gcacctcctc gagctggtcg cgggctcgtc gcggcgcacg gtcccggtgt cgctcgccaa
     3001 gggcgcccgc ctctcgcact tcgtcgagat gcccgccgcc agcaccagcg gcgagctgcg
     3061 cgtcaccaca gacccgcagg gcctgcgcgt gatcgtggac ggcaagctcc ggggcaccag
     3121 cccgctcctc gtctccgacc tcgccccggg gccccacgtg gtcgccgtcg agggcagggg
     3181 gcgttcggct cgccacgagg tgaaggtcga cgctggcgca accgccgccc tcttcgtgcc
     3241 gctctcctcg ggtggcgcgc cgtcctccgg ctggctgagc attgcgggcg agatcgagct
     3301 acaggtctac gagaacggcg agctggtcgg tacctcgcgc agcccccgga tcatgctgcc
     3361 cgccggtctc cacgaggtgg agctggtgaa cgaggagctg ggcgtgcgga tcggcgagga
     3421 ggtgcggatt tctccgggcg ggacctcctc gctcgaggtc gacctgccgc aaggccggat
     3481 ggacgtgaac gcgctgccct gggccgaggt ctggctggac ggcaagcgga ttggcgaaac
     3541 ccctctcggc ggcgttccgg cccgggtggg ccgtcacatg ctgacgttcc ggcacccgca
     3601 gctcggagaa cggcgcgtgg agtgcctcgt ggccgccggg cgcccgacgc gcgtcggcgt
     3661 ggacctgcgc aagtgaccga gccgatggcc taccagctcc tcgtcatgga cgacagcgtc
     3721 acgattcgcc gcgtcgtgga actgaccttc gcgggaggtg acgtgcaggt ggtgggtgtc
     3781 ggcgacggcc gggccgcgct ggagcggatc gcggccgaac cgcccgacgt catcctcgcc
     3841 gacgtcggca cctcgcagcc cgacggctac gaggtgtccg cgtacgtgaa aggaaacccg
     3901 gcgctcgcgc acatccccgt actgctgctg agcggcgcgt tccagccgat cgacgaggag
     3961 cgcgcccgcg ccgccggctg cgacggcgtg ctggcgaagc cgttcgaccc cggggcggta
     4021 gccgctcgcg tgcggcaggt gctcgaagaa cgcggggcaa agcccgagcc gtcgccgcca
     4081 tcggacgaga tgatcgccgc ccggtccgcc gatccggctg ccgcccccga cgaggacgcc
     4141 gacctgctcg ccttcttcga ggagctgctc gcgcaacccc tgctcgaggg gtctgatgac
     4201 gcgtgccggg aggaggccgc gtgccggccc gagccttgcg ccccggcccc actccccgct
     4261 cccggcggcc agtcgccgtt ctcgctcgac gactatttcg acctgctgga cgagatcctg
     4321 gccggaggcc ttctgcgttc cggccctgct acctccccat ctccttcacc aggtggcccg
     4381 ccccgtccac ctcgctcatc gtccagagca tgtagcggct gtccacgtgg atcgaccgcg
     4441 tcttgacggg ttcgaacagg tagtcggacg cgaccgactc gtaggtgcca tcgaacagca
     4501 gtcccaccag ctcgccgcgg gcgttcagcg tcggcgaccc ggagttgccg ccggtggtgt
     4561 ccaccgtgga aaggaagttc accggcacgt cgcccagttc gggcaccagg tacggcgtct
     4621 tcgctccgcc gcgcagcgcc tcgatcgcgt ggagctgggc cttcggggcg ttgaactccc
     4681 cctcgcccgt gtgcttctcg acgatcccct ccagcgtggt ctgcggctcg tagaagaggc
     4741 cgtcgcgggc ccgcacgccc ttcacctggc cgaaggtcac gcgcagggtg ctgttcgcgt
     4801 cgggcgccac gaggccgccg gcgcgcgcga gcatcgcctc catgtaacgc gggcgcagct
     4861 tcgcgtacgc gcccgcgagg tccttccccg cttcgcggat cgattcgttg agcggctcga
     4921 gcgcggcggc cagctcgatg aacggatcgt tcgacgcctt cagctcggcg gcgctcttct
     4981 cgagcagggc gaggcgcacg tcctggtcgg ccagccgggt tcccgcgtag aggcggttca
     5041 ggtacgtgtc gatcgcctgg gacacctcgg cctggctcct gcccgggcgc aggcccgcga
     5101 gcgtatcgag cggctcgatg cgctggccct ccggcagcga cgcggccttc cccatcgccc
     5161 acgacagcag cgcgcggtcc accgtggggt cgatggtccg ctgcagccgc tcctgcgcgg
     5221 cgcggaggcg gctccagtcg cgctcctgga agccctgatc gcgatcgatg tcgcgcttcg
     5281 gccgctggag cgacaggcgg tagagggtct gcgcggcgtt gaggtacacc gacgcgcccg
     5341 acaggccggc aagcgccgcg ttccgttcgc gcgtcttcag cgaggcctcg tgcagcgcct
     5401 gcattgcggg cagcacctcg ccgtactgct gccggcggcc cgggtcggcg gcaatccagt
     5461 cggccagcgc ccgctcggcc tgcagcttgc gctcgaggct gccgcccttc acgaggccct
     5521 cgagcacccc cttcgtgttg gtgaggccgt tgttcagccc ctggatg
//
LOCUS       DAOPQX010000240         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_15983_length_5563_cov_4.478032, whole genome shotgun sequence.
ACCESSION   DAOPQX010000240 DAOPQX010000000
VERSION     DAOPQX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15983_length_5563_cov_4.478032"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="PKK95_12195"
     CDS             complement(<1..1365)
                     /locus_tag="PKK95_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HOC19027.1"
                     /translation="MKGRFLPHPGSARRGREALAQAPGREGSVEARSCRCAHCGLPVP
                     ARLLREGGERQFCCSGCRQVYTLVHEWGFDQYYRIAGQQDAALGPAKVTGRSFDDFDD
                     ERVRAEATDPAGPGRLRTRLYLEGVHCAACVWLVEKLPAVLNGVDEVRLNAGTGVAEV
                     TWRPDRTRLSAIGRALDRLGYTPHVHRASRAQEARRAEDRAGLARVGVASACAMNLMF
                     LHGAIYAGEHSGMDSPYETFLRWLSFGVALPVILYSARPFYQTAVAGLRAGMVHIDLP
                     IAVALTVTFAASAVNTVRGSGALWFDSLAMLVAALLGARQLQRASQRSALERADSLRG
                     VAFLEFARRLEGGLPDAPAVEVPLAALVPGDRVEIRSGELVPVDGVVLSGRSSLDNAV
                     LTGEAAALPVKEGDAVYAGATNLGARLVVRVEAAGVTTRVGTLLAIVQEALARKPVLL
                     QTTDR"
     gene            complement(1362..1895)
                     /locus_tag="PKK95_12200"
     CDS             complement(1362..1895)
                     /locus_tag="PKK95_12200"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOC19028.1"
                     /translation="MALRVISEYRWPIYLGALLAMPVVATAVLVWVATRPDSPRPIAG
                     YYEAARAWDAGEAVAEASRQMGWTVRFELPPDIPHYPGMPRPVDVRVADRDGRPVSGL
                     AGSLFAVRPSDPHPDQSGRLTELPHQPGTYRTLVRIDEPGTWDLRLDARQQALRFVYA
                     ERLMVPADKALPAGGTR"
     gene            complement(1940..3418)
                     /gene="ccoG"
                     /locus_tag="PKK95_12205"
     CDS             complement(1940..3418)
                     /gene="ccoG"
                     /locus_tag="PKK95_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOC19029.1"
                     /translation="MEAAETTPTRAAALPLELAREAPVFDVAPEEELIYSLAADGRRK
                     FMHPVVHKGRYWKIRRAIAYALAAAFLGLPHVRVGGYPAVMLDLANRRTHVFGATFYP
                     TDTLILVSLGFGVIVTVFFVASSFGRMWCGYSCPQTVWLEFFFRPIEAFLEGGPYKQR
                     KLNDAPWSGRKIAIETAKWAAWTVLALLMSTTFVAYFAGWGPLAHGLATSPLAWTGPL
                     FAIGLLTTAIVFDFGWFRDQMCTIACPYGRLQNVLSDQDTILVAYDGKRGDPKVKLKD
                     RVEGVRAGDCIDCRACVNACPTGTDIRRGLQPECIGTAQCVDACNEVMTAQRKPIGLI
                     RYTSERQQKGGARHLWRARTLVYLALMAVAWGSLGTLVLTRADALVEIVRGGREAYRL
                     LPGGEVANQQRVRVTNQIAETQRFTIELLGPAGARLIVADSPIVAAPDAVVTVNMVTT
                     VPQSAFTDGQVSARYLVRSDRGFRKEMEFMLLGPFSSAGGKP"
     gene            complement(3420..4010)
                     /locus_tag="PKK95_12210"
     CDS             complement(3420..4010)
                     /locus_tag="PKK95_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOC19030.1"
                     /translation="MPEFEDKVLHELDGIREYDNPMPGWLMAIFWGSLVFSAAYLIFY
                     ALSFGEGTMEAEYRRETERALASVQAHFDANPLVAPSAAALLAGAADPAVLQVAEARF
                     ARSCASCHGERAEGLIGPNLTDDRWIHGGSVDQIFQSIAKGWPAKGMPPWGRALKPDE
                     LAALASYVRSLQGSNPPNARPPEGDPVAPEPIPPRS"
     gene            complement(4030..4227)
                     /locus_tag="PKK95_12215"
     CDS             complement(4030..4227)
                     /locus_tag="PKK95_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19031.1"
                     /translation="MLQELAARSDTAAWAIGSMLFFLAVFVAIVVWVVRKHPEEMARR
                     ARMPLENGDQESGTGNRDSEV"
     gene            complement(4240..>5563)
                     /gene="ccoO"
                     /locus_tag="PKK95_12220"
     CDS             complement(4240..>5563)
                     /gene="ccoO"
                     /locus_tag="PKK95_12220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HOC19032.1"
                     /translation="PILKFMVVAVTYYGMSTFEGPMLAIKSVNAVSHYTDWTIGHVHA
                     GTLGWNAFLSFGILYWVVPRLWKTELYSTRLATTHFWIATVGLILYQVSMWVAAITQW
                     GMWRAFEPDGRLVYPDFIETVIAIVPMYWVRLVGALLFFTGALLLVWNLFRTIRSAPA
                     GYAEEPEIVAPPLVRDVSAPGAVTPENTYDHAFYRLQYALRHGVHRFLESVPVPFTVL
                     VVVAVSVGSLVEAIPMFFDKGNVKEIASVKPLTPLEVAGRDIYIREGCYNCHSQMVRP
                     FRYETERYGEYSKAGEFVYDHPFQWGSKRTGPDLHRVGGKYPSLWHVRHMRQPDSTTP
                     GSVMPRYPHLLADPYDTRLAPSKLRALRTVGVPYTDGEIETAVEAMAIQARAIAAEIE
                     AQQGPQGLDGKEITALTAYLQRLGTDIRWKRSASAPEAAGASAGQQAR"
BASE COUNT          845 a         1950 c         1995 g          773 t
ORIGIN      
        1 gcggtcggtc gtctgcagca gaacgggctt cctcgcgagc gcctcctgga cgatggccag
       61 cagcgttccg actctcgtcg tcacgccggc ggcctcgacg cggacgacga gccgtgcgcc
      121 aaggttggtg gctcccgcgt agaccgcgtc gccctctttc acgggcagcg ccgccgcctc
      181 gcccgtgagg accgcgttgt cgagggagga gcggcccgag aggacgaccc cgtcaaccgg
      241 gaccagctcg cccgatcgga tctcgacccg gtcgcccggc acgagagccg cgagcgggac
      301 ctcgacggcc ggtgcgtcgg ggaggccgcc ctcgaggcgt cgcgcgaact ccaggaaggc
      361 cacgccgcgc aggctgtcgg cccgctcgag cgccgagcgc tggctggcac gctgcagctg
      421 ccgggcgccg aggagggccg ccaccagcat cgcgagcgaa tcgaaccaga gtgcaccgct
      481 gccccggacc gtgttcaccg cgcttgcggc gaacgtcacc gtcagcgcga ccgcgatggg
      541 caggtcgatg tggaccatcc ccgccctgag gccggccacg gccgtctggt agaacgggcg
      601 cgccgagtag aggatgaccg gcagggcgac gccgaacgaa agccagcgca ggaaggtctc
      661 gtaggggctg tccatgcccg agtgttcgcc ggcgtagatc gcgccgtgca ggaacatcag
      721 gttcatcgcg caggcggagg cgacgccaac ccgtgcgagg ccggcccggt cctccgcccg
      781 ccgcgcctcc tgggcgcggg aggcgcggtg gacgtgcggc gtgtagccga ggcgatcgag
      841 cgcgcggccg atggccgaga ggcgcgtgcg atccggccgc caggtgacct ctgcgacgcc
      901 ggtgccggcg ttcaggcgga cctcgtccac cccgttcagg acggcgggaa gcttctcgac
      961 gagccagacg caggcggcgc agtgcacgcc ctcgagatac agccgcgtcc tgagacgccc
     1021 gggcccagcc ggatcggtcg cctcggcccg cacccgctcg tcgtcgaagt cgtcgaacga
     1081 gcggcccgtc accttcgccg ggccgagcgc tgcgtcctgc tggcccgcga tgcggtagta
     1141 ctggtcgaac ccccactcgt gcaccagggt gtagacctgc cggcagccgc tgcagcagaa
     1201 ctgccgttcg ccgccctcgc gcagcagcct ggcgggaacg gggagaccgc agtgcgcgca
     1261 ccggcaggac cgcgcttcga cgctgccctc gcgtcccggg gcctgggcaa gggcttcacg
     1321 gccacgacgg gcgctgcctg gatgcggcag gaagcgaccc ttcaccgcgt cccccccgcg
     1381 gggagggcct tgtcggccgg caccatcagc cgctccgcgt agacgaaccg gagcgcctgc
     1441 tggcgcgcat cgaggcgcag gtcccacgtg ccgggctcgt cgatccggac gagcgtccgg
     1501 tacgtcccgg gctggtgcgg cagctcggtg aggcgtcccg attggtccgg gtgcgggtcg
     1561 gaggggcgaa cggcgaacag gctccctgcc agcccagaga ccggccttcc gtcgcgatcg
     1621 gcaacccgga cgtccaccgg tcgcggcatg cccgggtagt gggggatgtc cggcggcagc
     1681 tcgaaccgga ccgtccaccc catctgccgg cttgcctcgg cgaccgcctc gccggcgtcc
     1741 cacgccctgg cggcctcgta gtagcccgcg atggggcggg ggctgtcggg ccgagtcgca
     1801 acccacacga gcaccgcggt ggcgacgacc ggcatcgcga gcagcgcgcc aaggtagatc
     1861 ggccagcggt actcggagat gactctcagg gccaaacgga tccttccgcc ctccgccctc
     1921 cgcgctcccc ctacggccgt catggctttc ccccggcgga ggagaacggc ccgagcagca
     1981 tgaactccat ttccttccgg aagccgcgat cggagcggac gaggtaccgt gcggacacct
     2041 gcccgtccgt gaaggcggat tgcggcaccg tggtgaccat gttgaccgtc accacggcgt
     2101 cgggcgcggc cacgatcggc gagtcggcga cgatcagccg cgcgccggcc ggcccgagaa
     2161 gctcgatggt gaagcgctgc gtctcagcga tctggttggt cacgcgcacg cgctgctggt
     2221 tcgcaacctc accgcccggg agcagccggt aggcctcgcg tccgccgcgc acgatctcga
     2281 ccagcgcgtc ggcgcgtgtc aggaccaggg tgccgagaga cccccacgcc acggccatca
     2341 gtgccaggta gacgagcgtg cgagcgcgcc agaggtgcct ggcgcctccc ttctgctggc
     2401 gctcggaggt gtagcggatc aggccgatcg gcttgcgctg cgccgtcatc acctcgttgc
     2461 acgcgtcgac gcactgcgcc gtgccgatgc actcgggctg caggccgcgg cggatgtcgg
     2521 tgcccgtcgg gcacgcgttg acgcaggcac ggcagtcgat gcagtcgccg gcgcgaaccc
     2581 cttccacgcg gtccttcagc ttgaccttcg gatcgccccg cttcccgtcg tatgccacca
     2641 ggatggtgtc ctggtcggac agcacgttct gcaggcggcc gtaggggcag gcgatcgtgc
     2701 acatctgatc gcggaaccag ccgaagtcga agacgatggc ggtggtgagc aggccgatcg
     2761 cgaacagggg ccccgtccag gcgagcgggc tcgtggcgag cccgtgggcg agcgggcccc
     2821 acccggcgaa gtacgcgacg aaggtcgtgg acatcagcag ggcgaggacc gtccaggccg
     2881 cccacttcgc cgtctcgatc gcgatcttgc ggccgctcca cggcgcgtcg ttcagcttcc
     2941 gctgcttgta gggcccgcct tccaggaacg cctcgatcgg gcggaagaag aactcgagcc
     3001 agaccgtctg cgggcacgag tagccgcacc acatgcggcc gaacgacgag gccacgaaga
     3061 acacggtgac gatcacgccg aagccgagcg acaccagaat gagggtgtcg gtggggtaga
     3121 aggtcgcgcc gaacacgtgc gtccggcggt tggcgaggtc gagcatgacg gccgggtagc
     3181 cgcccaccct gacgtgcggc aggccgagga acgcggcggc gagggcgtac gcgatcgccc
     3241 ggcggatctt ccagtaccgc cccttgtgga cgaccgggtg catgaacttc cgcctgccgt
     3301 ccgcggcgag gctgtagatc agctcctcct cgggcgcgac gtcgaagacg ggcgcctccc
     3361 tcgcgagctc gaggggcagg gcggccgccc gcgtcggggt ggtctcggca gcctccacgt
     3421 cagctcctcg gcgggatcgg ctcgggtgcg acagggtcgc cctccggcgg gcgggcattc
     3481 ggcgggttcg agccctgcag gcttcgcacg tacgaggcca gcgccgcaag ctcgtcgggc
     3541 ttcaaagcgc gtccccaggg cggcatgccc ttcgccggcc agcccttcgc gatcgactgg
     3601 aagatctggt cgactgagcc gccgtgaatc cagcgatcgt cggtcaggtt cgggccgatc
     3661 agcccctccg ccctctcgcc gtggcacgag gcgcaggagc gggcgaagcg ggcctcggcg
     3721 acctggagta cggccggatc ggcggcgccg gcgagcagtg cggcggccga gggcgccacc
     3781 agcgggttgg cgtcgaagtg ggcctggacg gacgccaggg cccgctccgt ctcgcgccgg
     3841 tactccgcct ccatcgtgcc ctcgccgaag ctgagcgcgt agaagatcag gtaggccgcc
     3901 gagaagacga gcgaacccca gaagatcgcc atgagccagc cgggcatcgg gttgtcgtac
     3961 tcccggatcc cgtcgagctc gtgaaggacc ttgtcttcga actccggcat ggtgcgcctc
     4021 ggctgggagc tagacttccg aatcccgatt cccggttccc gactcctgat ccccgttctc
     4081 caacggcatc cgggcgcgcc tcgccatctc ctcggggtgc ttgcgcacga cccaaaccac
     4141 gatggcgacg aagaccgcca ggaagaacag catcgagccg atggcccacg cggccgtgtc
     4201 gctgcgggcg gccagctcct gcaacacgcc ccacctcctc taccgggctt gttgccccgc
     4261 cgaagcgccc gcggcttcgg gcgccgaggc ggaccgcttc caccggatgt cggtgccgag
     4321 acgctgcagg tacgccgtca gcgccgtgat ctccttgccg tcgagcccct gggggccctg
     4381 ctgcgcctcg atttccgccg cgatcgcccg ggcctggatc gccatcgcct cgacggccgt
     4441 ctcgatctcc ccgtcggtgt aggggacccc gaccgtgcgc agcgcccgca gcttggaggg
     4501 ggccagccgc gtgtcgtacg ggtcggcgag caggtggggg tagcgcggca tcaccgagcc
     4561 gggcgtggtc gagtcgggct ggcgcatgtg cctgacgtgc cagagcgagg ggtacttgcc
     4621 gccgacccgg tgcaggtcgg gccccgtgcg cttcgagccc cactggaacg ggtggtcgta
     4681 gacgaactct ccggccttcg agtactcgcc gtagcgctcg gtctcgtagc ggaagggccg
     4741 gaccatctgc gagtggcagt tgtagcagcc ctcgcggatg tagatgtcgc ggccggccac
     4801 ctcgagcggc gtgagcggct tgacgctggc gatttccttg acgtttccct tgtcgaagaa
     4861 catcgggatc gcctcgacca gtgagccgac cgagaccgcg accacgacca ggacggtgaa
     4921 cggcaccggg acgctctcga ggaaccggtg cacgccgtgg cgcagcgcgt actgcaggcg
     4981 gtagaacgcg tggtcgtagg tgttctcggg cgtcacggcg ccgggcgccg acacgtcgcg
     5041 cacgagcggc ggggcgacga tctccggctc ctccgcgtag ccggccggtg cgctgcggat
     5101 cgtccggaag aggttccaca ccagcaggag ggcgcccgtg aagaagagca gcgcccccac
     5161 gaggcgaacc cagtacatcg gcacgatcgc gatgacggtc tcgatgaagt cggggtagac
     5221 gaggcggccg tcgggctcga acgcgcgcca catcccccac tgcgtgatgg ccgccaccca
     5281 catcgagacc tggtagagga tcaggcccac ggtcgcgatc cagaagtggg tggtcgccag
     5341 ccgcgtcgag tacagctcgg tcttccagag ccgcgggacg acccagtaga ggatgccgaa
     5401 cgaaaggaag gcgttccagc cgagcgtccc ggcgtggacg tggccgatgg tccagtcggt
     5461 gtagtgcgag acggcgttca ccgacttgat cgccagcatc ggcccttcga aggtggacat
     5521 gccgtagtag gtgacggcga ccaccatgaa cttgaggatc ggc
//
LOCUS       DAOPQX010000241         5504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16212_length_5504_cov_4.825473, whole genome shotgun sequence.
ACCESSION   DAOPQX010000241 DAOPQX010000000
VERSION     DAOPQX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16212_length_5504_cov_4.825473"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /gene="glmS"
                     /locus_tag="PKK95_12225"
     CDS             complement(<1..1555)
                     /gene="glmS"
                     /locus_tag="PKK95_12225"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOC19033.1"
                     /translation="MCGIIGYIGRKPVVPVLIEGLRRLEYRGYDSAGVALVRNGSVAL
                     RRSAGKLSRLEDSIRLDPIDGDYGVGHTRWATHGRPTEENAHPHRDCTGRIVVVHNGI
                     IENYLELKQDLQRKGHKFVTETDTEIVAHLIEEELKRKAGTEAGAAGTTLEDATRCAL
                     ARLRGMFALVILSADEPQRIVAVRKGPPLVAGVGDGEYFVASDIPAILAHTREVVFLG
                     DDEMVVLTRAGLDFTNFAGTRLERRTQRVLWDPIQAEKAGFKHFMLKEIFEQPQSVRD
                     TVLGRVSGESGRVFLEEMTIPEAELAEVENVSIIACGTSWHAGLVGKYLIESLARLPV
                     ETDYGSEYRYRNPITGSKTLAVVITQSGETADTLAALREARQKGARSVAICNVVGSMA
                     TRESDGTVYTHAGPEIGVASTKAFTSQLVALYLLALHLAQVRGTITPEDSREHIDGLL
                     ALPTLIDQTLRVSALVDEIARRFYGRRDFLFLGRGLNYPIALEGALKLKEISYIHAEG
                     YPAGEMKHGP"
     gene            complement(1600..3012)
                     /gene="glmU"
                     /locus_tag="PKK95_12230"
     CDS             complement(1600..3012)
                     /gene="glmU"
                     /locus_tag="PKK95_12230"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF010934.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOC19034.1"
                     /translation="MPSLHILILAAGKGTRMKSALPKVLHPLAGAPIIEYVLATAAAL
                     SPASRTIVVGHLAERMREALAAHGDLRFVVQEPQLGTAHAVAQAAPLLEGATGTLLLL
                     YGDVPRLSVQTVRALLDEHERAGNAATVLTAVVPDPSGYGRIIRDPGGIARIVEHRDA
                     TPDERAVREINSGIYAFELEPLFPALSRIATDNDQGEYYLTDLVAIYRREGKRVGAVV
                     AERPDEILGINGRGELAAMAKRIWRERAEALMASGVTLEDPDTVYVDAGVVVGRDTTI
                     RPGVILEGRTTIGANCVIHPGVHIEDSTLEDNVVVFDYSVITGAQIAEHAEVGPFARL
                     RPEAEVGREAKIGNFVELKKTRLGAGSKAMHLAYLGDATIGGNVNIGAGTITCNYDGV
                     RKNPTTIEEGAFIGSDSQLVAPVKVGRGAYVAAGSSITRDVPAGALGIARGRQENHEG
                     WVERRKRKQAEAAERKPEKT"
     gene            complement(3131..3661)
                     /locus_tag="PKK95_12235"
     CDS             complement(3131..3661)
                     /locus_tag="PKK95_12235"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19035.1"
                     /translation="MAARTRPPLPPPPPPPDPSRKAVELFERGFRALQQRDYAKAAEI
                     LASLVTSYPDEKELHERARVFLAICERQAAAAPQPQTLEERVCAATLAINRGETGQAL
                     RMLADLAREHPDHDHVHYLLAIAHGAAGHTGDAVAHLRRAIELRPSNRVLAGQDADLE
                     ALRDDPGFHALLNGSR"
     gene            complement(3978..4829)
                     /gene="rsmI"
                     /locus_tag="PKK95_12240"
     CDS             complement(3978..4829)
                     /gene="rsmI"
                     /locus_tag="PKK95_12240"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOC19036.1"
                     /translation="MPGTLFVVATPIGNLEDITSRALRVLREAALIAAEDTRHTGRLL
                     AHFAIPTPTTSFYEQNEREKLPRLLERLRAGDNIALVSDAGTPAVSDPGYRLVQAAAA
                     GGIRVEAIPGPSAILAALVASGLPTNAFTFLGFPPARAAARDRWLADLGARRETTIFF
                     EAPHRVRATLEAAARILGSRPAAICRELTKLHEEVLRGPLADLVRSLPNPRGEFTIVI
                     GPAEGAEAQAGLPGPDELLTEFGQLTDEGLDRRAAISELAKRYQVSSREVYAAVEQAR
                     AAAEGLP"
     gene            4983..>5504
                     /locus_tag="PKK95_12245"
     CDS             4983..>5504
                     /locus_tag="PKK95_12245"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HOC19037.1"
                     /translation="MYSSEPLHHFVDDYLRYLHETHPTSAALDGVHTHDDLLEDMSRT
                     ALEGQARTLAGFSRRLDEIGLDGLSAGERVEHPVIASNIRARIHEIEEVRAWEKNPQV
                     YADLLATSLATQAIFPYAPEDERARRVFSKLRQTPRLIQAARENVKEPPGIYVKVALE
                     TLRGVEKFIEHDLP"
BASE COUNT          748 a         1828 c         2064 g          864 t
ORIGIN      
        1 tcggcccgtg cttcatctcg ccggccgggt agccctcggc gtggatgtag gagatctcct
       61 tgagcttgag cgccccctcg agcgcgatcg ggtagttgag cccccggccg aggaagagga
      121 aatcgcggcg cccgtagaag cggcgggcga tctcgtccac cagcgccgag acgcgcagcg
      181 tctggtcgat gagcgtcggc agcgccagca ggccgtcgat gtgctcgcgc gagtcttcgg
      241 gcgtgatcgt gccgcgcacc tgcgccaggt gcagcgccag caggtagagc gccacgagct
      301 gcgaggtgaa cgccttggtg gaggcgacgc cgatctcggg cccggcgtgc gtgtagaccg
      361 tgccgtcgct ttcgcgcgtg gccatgctgc cgaccacgtt gcagatggcg acgctgcggg
      421 cccccttctg ccgcgcctcg cgcagggccg ccagcgtgtc ggcggtctcg cccgactggg
      481 tgatcaccac cgcgagcgtc ttcgagccgg tgatcgggtt gcggtagcgg tactccgagc
      541 cgtagtcggt ttcaaccggc aggcgggcga gcgactcgat caggtacttg ccgaccagcc
      601 ccgcgtgcca cgaggtgccg cacgcgatga tcgagacgtt ctcgacctcc gccagctcgg
      661 cctcggggat cgtcatctcc tcgaggaaga cgcggccgct ctcgcccgag acccgtccga
      721 gcaccgtgtc gcggaccgac tgcggctgct cgaagatctc cttgagcatg aagtgcttga
      781 agcccgcctt ctcggcctgg atcgggtccc acagcacgcg ctgcgtgcgg cgctcgaggc
      841 gggtgccggc gaagttggtg aagtcgaggc cggcccgcgt gagcaccacc atctcgtcgt
      901 cgccgaggaa cacgacctcg cgggtgtgcg cgaggatggc ggggatgtcg gaggcgacga
      961 agtactcgcc gtcgccgacg ccggccacca gcggcggccc cttgcggacg gcgacgatcc
     1021 tctgcggctc gtcggccgag aggatcacga gggcgaacat cccgcgcagc cgcgcgagcg
     1081 cacagcgggt cgcgtcctcg agagtggtgc cagcagcgcc ggcctccgtc ccggccttcc
     1141 tcttcagctc ctcctcgatc aggtgcgcaa cgatctccgt gtcggtctcg gtgacgaact
     1201 tgtggccctt gcgctgcagg tcctgcttca gctcgaggta gttctcgatg atcccgttgt
     1261 ggacgacgac gatgcggccg gtgcagtcgc ggtgagggtg ggcgttttcc tcggtggggc
     1321 gtccgtgcgt ggcccagcgc gtgtggccga cgccgtagtc gccgtcgatc gggtcgaggc
     1381 gaatcgagtc ttcgaggcgc gacagcttgc ccgcgctgcg gcgcagggcc acgctcccgt
     1441 tccggaccag ggcgacgccg gccgagtcgt aaccccggta ctcgaggcgg cgaagcccct
     1501 cgatgaggac cgggacgacc ggcttccgcc cgatataccc gataattccg cacacttggg
     1561 actccagggg ctgggggcca ggagctaggg gtcccccgcc tacgtctttt ccggtttccg
     1621 ctccgcggcc tcggcctgct tccgtttcct ccgctccacc cagccctcgt ggttctcctg
     1681 gcggccgcgg gcgatgccga gggcgccggc cgggacgtcc ctggtgatcg acgagccggc
     1741 ggcgacgtag gcgccgcgcc ccaccttgac cggggcgacg agctggctgt cgctgccgat
     1801 gaacgccccc tcctcgatcg tcgtcgggtt cttgcgcacc ccgtcgtagt tgcaggtgat
     1861 ggtcccggcg ccgatgttga cgttgccgcc gatcgtcgcg tcgccgaggt aggccaggtg
     1921 catcgccttc gatccggcgc cgaggcgcgt cttcttcagc tccacgaagt tgccgatctt
     1981 cgcctcgcgg cccacctcgg cctcgggcct gagccgggcg aacggaccga cctccgcatg
     2041 ctctgcaatt tgcgcgccgg tgatgaccga gtaatcgaac accacgacgt tgtcttccag
     2101 ggtgctgtcc tcgatgtgaa ccccggggtg gatgacgcag ttggcgccga tcgtcgtccg
     2161 cccctccagg atcacccccg gccggatggt cgtgtcgcgc ccgacaacca ctcccgcgtc
     2221 cacgtagacg gtgtcggggt cctcgagggt gacgccggac gccatcagcg cctcggcccg
     2281 ctcgcgccag atccgcttgg ccatcgcggc cagctcgccg cggccgttga tccccaggat
     2341 ttcgtcgggc cgctcggcca ccacggcgcc cacgcgcttc ccctcgcgcc ggtagatggc
     2401 cacgaggtcg gtgaggtagt actcgccctg atcgttgtcg gtggcaatgc gagacagggc
     2461 ggggaagagc ggctcgagct cgaaggcgta gatgccggag ttgatctcgc ggacggcacg
     2521 ctcgtcgggg gtggcgtcgc ggtgctcgac gatgcgggcg attccgcccg ggtcgcggat
     2581 gatgcggccg tagccggagg ggtcggggac gaccgcggtc aggacggtgg ccgcattgcc
     2641 ggcgcgctcg tgctcgtcga ggagcgcgcg cacggtttgc accgacaggc gggggacgtc
     2701 gccgtacagg agcaggaggg tgccggtggc gccctcgagc agtggggcgg cctgggcaac
     2761 cgcgtgagcg gtgccgagct gcggctcctg gacgacgaag cggaggtcgc cgtgcgcggc
     2821 gagcgcctcg cgcatcctct ccgcgaggtg gcccaccacg atcgtgcggc tggcgggcga
     2881 cagggcagcg gcggtggcga ggacgtactc gatgatcgga gcgccggcca gggggtggag
     2941 caccttcggc agcgccgact tcatgcgggt ccccttgccg gcggccagga tcagaatgtg
     3001 gagagaaggc atagggacca tagtaaggca gtccacgaag tcgcgaagtc gcgaagtcac
     3061 gaagtcgagt ccacgggggc gacggtcggg cggtcgccct ggtcgccaac ccgagacccc
     3121 gtggactggt ctaccggctg ccgttcagca gcgcgtggaa tccggggtcg tcgcggagag
     3181 cttcgaggtc ggcgtcctga cccgccagca cgcggttcga cgggcgcagc tcgatggcgc
     3241 ggcggaggtg ggcgacggca tcgccggtgt ggccggccgc gccgtgcgcg atggcgagga
     3301 ggtagtggac gtggtcgtgg tcggggtgct cgcgagccag gtcggcgagc atccggaggg
     3361 cctgccccgt ctcgccccgg ttgatggcga gcgtcgcggc gcagacgcgc tcctcgagtg
     3421 tctgcggctg gggtgccgcc gccgcctggc gttcgcagat ggccaggaac acccgggccc
     3481 gctcgtgtag ttccttctcg tcggggtagg aagtcaccag cgaggcgagg atctcggccg
     3541 ccttggcgta gtcgcgctgc tggagcgcgc ggaagccccg ctcgaacagc tcgacggctt
     3601 tgcggctggg gtcgggtggc ggcgggggag ggggaagcgg tggcctcgtc cttgctgcca
     3661 cggcgggcgc ggcggccttg gcacgcgccg gtttcgcctt tggtgccttc ggtttggcgg
     3721 ccttggcatg gctcgccgcc cgtgatggct tcgccgtttt cttcaccggc ttccgcgggg
     3781 cggcaggtcg ggcacgcttg gccatgtcga tgcgacggat gccgcgctac cttaacaaaa
     3841 cgcaagaggg cgggtcaacc cttcgactcg gcagcccttc gcaggcctcg ctcggggcag
     3901 ggctcaaggc tcagggctca agggcttgct gagccgtagg ggcgaccttg tggtcgtcct
     3961 cgcacgcggc gacagaatca gggcaggccc tcagccgccg cccgtgcctg ctcgacggcc
     4021 gcgtaaacct cgcgcgagga gacctggtag cgcttcgcca gctcgctgat ggcggcgcga
     4081 cggtcgaggc cctcgtcggt cagttgcccg aattcggtga gaagctcgtc cgggcctggc
     4141 agtccagcct gcgcttccgc cccctcggcc gggccgatca cgatggtgaa ctcgccgcgg
     4201 gggttgggga gcgaccggac gaggtcggcg aggggccccc gcagcacctc ctcgtgcagc
     4261 ttggtcagct cgcggcagat ggcggccggg cggctgccga ggatgcgggc ggcggcctcg
     4321 agcgtggccc gcacccggtg cggcgcctcg aagaagatcg tggtttcgcg gcgggcgccg
     4381 aggtcggcca gccagcggtc gcgggcggcg gcgcgggcgg gggggaagcc gaggaaggtg
     4441 aaggcattgg tgggcaggcc ggatgcgacc agcgcggcga ggatggcgct tgggcccggt
     4501 atcgcctcga cgcggatgcc cccggcggcg gcggcctgca ccaggcggta gcccggatcg
     4561 gagacggccg gcgtgccggc gtccgagacg agggcgatgt tgtcgccggc gcgcagccgc
     4621 tcgagcaggc gggggagctt ctcccgctcg ttctgctcgt agaagctggt ggtcggcgtc
     4681 gggatggcga agtgggccag caggcggccg gtgtggcgtg tgtcttcggc ggcaatcagg
     4741 gccgcctcac ggagcactcg aagcgcgcgg gaggtgatgt cctcgaggtt tccgatgggg
     4801 gtggcgacga caaacagcgt gccaggcata cacggccgtt cgattattgt accattggtg
     4861 ggttggcctc cctgggaccg cccgggggca ggccgccagg ttcaggccgc gcgggtcggc
     4921 ggcctgctca ccgttcgggc cgcgcaaccg gaacgcggcc gtcgagacgc gggggcgcga
     4981 gcgtgtattc atccgaaccg ctgcatcact tcgtggacga ttacctgcgg tacctgcacg
     5041 agacccaccc gacctcggcg gcgctcgacg gggtgcacac ccacgacgac ctgctcgagg
     5101 acatgagccg cacggcgctc gaggggcagg cccgcacgct ggccgggttc agccggcgcc
     5161 tcgacgagat cggcctcgac gggctgtcgg ccggtgagcg cgtcgagcac ccggtgattg
     5221 cctcgaacat ccgggcgcgg atccacgaga tcgaagaggt gcgcgcctgg gagaagaacc
     5281 cccaggttta cgccgacctg ctcgcgacca gcctcgcgac gcaggcgatc ttcccgtacg
     5341 ctccggaaga cgagcgggca cgccgggtgt tctcgaagct gaggcagacg ccgcggctca
     5401 tccaggccgc ccgcgagaac gtcaaggagc cgcctggcat ctacgtcaag gtggcgctcg
     5461 agacgctgcg cggcgtggag aagttcatcg agcacgatct gccg
//
LOCUS       DAOPQX010000242         5484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16278_length_5484_cov_4.104623, whole genome shotgun sequence.
ACCESSION   DAOPQX010000242 DAOPQX010000000
VERSION     DAOPQX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16278_length_5484_cov_4.104623"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKK95_12250"
     CDS             <1..887
                     /locus_tag="PKK95_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC19038.1"
                     /translation="LDFHAGPRSLAGGLLDGPGGAVLVDPGPASALGGLRRGLAEHGR
                     SVADLQAVLLTHVHLDHAGAAGVLARENTRLRIFVHERGAPHLADPSKLVASASRIYG
                     DRMEELWGEVAPVDPARIEPLQGGERLRVAGRDIEVAYTPGHAWHHASYLDRETGTAC
                     VGDVAGVRFAPSDEVIPPTPPPDIDVERWHQSLDQVAAWSPSRLLLTHFGTFDDVGSH
                     LERVRTRLDEMAGLSRQSLEGPERDDGERMAWFRARLRESLAARLGADAAAAYEAAQP
                     LEHYWQGLARYWRRRPGR"
     gene            1030..1344
                     /locus_tag="PKK95_12255"
     CDS             1030..1344
                     /locus_tag="PKK95_12255"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOC19039.1"
                     /translation="MADERQKPGIEIAERPEQATSRPRMFQVVLHNDDYTTMDFVVEV
                     LEHVFLKSPAEAFRIMMEVHRNGRGVGGVYPHEIAETKSAMVQSLARERGFPLQTSVE
                     EA"
     gene            1366..3699
                     /locus_tag="PKK95_12260"
     CDS             1366..3699
                     /locus_tag="PKK95_12260"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC19040.1"
                     /translation="MFSAAVEMLLHIAYREAVSRRHTTLTLEHLLYVLTHDLEGERIL
                     AACGADLPRLRRELDTFLQSSVEQFPRGMEKEPEQTRAFRRVLQTAVLHVQSAGKPEV
                     NAGDIVAAMLQQTNAYSCQLLEAQGVTRLDVLNYISHGISKVPVPPTPGEEGRRRGQP
                     QPGVEGQAGSSRDPLASYAVNLTERARAGLLDPLIGRSVELQRTMEILCRRRKNNPVF
                     VGDAGVGKTALAEGLAIRLLESTVPPILEGAEIYSLDTAALLAGTRFRGDFEERFKAV
                     LAALKSRRLPILFIDEIHGTIGAGATTGGTMDLATLIKPVLTAGELRVVGSTTFEEFK
                     QIEKDRALARRLQKVVVEEPSVEETIRILQGLRSRYEEHHQVEYEDAALEAATRLAKR
                     FLREYRLPDSAIDILDEAGAMLRLQGPAAPGGPGAAEAAPRRVTPAEIERVVGRMARI
                     PDKQATSSEKERLRTLEASLRRVVFGQDEAVRIVAAAIKRSRAGLAQPDRPAGCFLFT
                     GPTGVGKTELARQLALHLGNEFLRYDMSEFMEKHAVSRLIGAPPGYVGFEQGGLLVDA
                     VRTHPYAVVLLDEIEKAHPDMFNILLQVMDHATLTDNNGRRADFRQVVLIMTSNAGSR
                     EMSAASIGFGGGGASAAQEAGLRDQASRSRARTAIERVFSPEFRNRLDATVTFAALSP
                     SVMETIVEKFILQLESQLSERRIEIALEPAARAWLARRGYDPIYGARPLARVIQSEVR
                     DPLTDEILFGRLEHGGTVRIGLEDDHLTFAFAEAAAQ"
     gene            3736..4356
                     /locus_tag="PKK95_12265"
     CDS             3736..4356
                     /locus_tag="PKK95_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOC19041.1"
                     /translation="MNDDKTNGKEREPVDGPVKVIDRRWWTHGETGAPVEPPRKPTYL
                     EELERQIAEKDQAIQAYASKYRESAAEFDQVRARLRRDLDRDIERARRAVLADFLEIA
                     DNLDRALAAAPAGTDEAFVRGVDLVRQLLLARFAAHGVTPMDAEGKRFDPAWHDAVSL
                     MEVADPGQDEVVQAVVKKGYLVGGEVLRPAAVVVGKYAGQGEAPTP"
     gene            complement(4381..4932)
                     /locus_tag="PKK95_12270"
     CDS             complement(4381..4932)
                     /locus_tag="PKK95_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOC19042.1"
                     /translation="MTYDIVLAGFGGQGILFLGKMIATAGMIEGKEVSWIPSYGPEMR
                     GGTANCSVVVSDKRIGTPVVSRPNVLVAMNRPSLEKFEPKMAEGGFLLINRTLIEIPH
                     TRTDIEVAYIGITGIAGGLGNPRLANVVALGALIARVPIVGRDSALAALRKELSGKKA
                     ALLDLNFAALDAGLQAAAQTVVS"
     gene            complement(4929..>5484)
                     /locus_tag="PKK95_12275"
     CDS             complement(4929..>5484)
                     /locus_tag="PKK95_12275"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC19043.1"
                     /translation="AHGRAPAVGTGLKRANPDSIVISYQGDGDLASSGTAEIIHAAAR
                     GENFTVVFINNAIYGMTGGQMAPTTLVGQASATSPYGRDPKFAGHPLHVSEMLATLKA
                     PRYIERTALNNVANLTRTKRAIKKALDLQQKGEGFTFVEVLATCPTNWGVDPNEAVDW
                     LESSLMPEYPLGVYRDAVPGEVKL"
BASE COUNT          828 a         1918 c         1969 g          769 t
ORIGIN      
        1 atctcgactt ccacgccggt ccccgctcgc tcgccggcgg cctgctggac ggcccgggcg
       61 gcgcggtgct cgtcgacccc ggaccggcca gcgcgctcgg cggcctgcgg cggggcctgg
      121 ccgagcacgg acgctcggtg gccgacctgc aggccgtcct gctgacccac gttcacctcg
      181 accatgccgg cgcagccggc gttctcgcgc gcgagaacac ccggcttcgc atcttcgtcc
      241 acgagcgggg cgcgccgcac ctcgccgatc cctcgaagct ggtggcgagc gcctcgcgga
      301 tctacgggga tcggatggag gagctctggg gcgaggtggc gccggtggat cccgcccgga
      361 tcgagcccct gcaggggggc gagcgtctcc gcgtggcggg ccgcgacatc gaggtggcgt
      421 acacgcccgg ccatgcctgg caccacgcgt catatctcga ccgcgagacc gggacggcat
      481 gcgtcggcga tgtcgccggc gtccggttcg ccccgtccga cgaggtcatt cccccgacgc
      541 cgccgcccga catcgacgtg gagcggtggc accagtcgct cgaccaggtt gcggcctggt
      601 cgccttcgcg gctgctgctg acccacttcg ggaccttcga tgacgtgggg tcccacctcg
      661 aacgggtgcg cactcgcctc gacgagatgg ccggcctctc ccgtcagtcg ctcgaggggc
      721 cggaacgtga cgacggcgag cggatggcgt ggttccgggc gcgcctgcgc gagtcgctgg
      781 ccgcccggct gggcgccgac gcggccgccg cctacgaggc ggcacagccg ctggagcact
      841 actggcaggg gctggcgcgc tactggcgcc ggcggcccgg ccgctgacac tccttgccca
      901 taccccgggc tgcccggaac gccgcgccag gccaccacgc ggcgccgccg gcggaggcgc
      961 actcgcgcaa gctatcccgt atactggagt gaccgatcac aaccgacaac ccccggacgg
     1021 acggagggga tggccgacga acggcagaaa ccaggcatcg agatcgcgga gaggcccgag
     1081 caggcgacct cgcggccgcg catgttccag gtcgttctgc acaacgacga ctacacgacg
     1141 atggatttcg tcgtggaagt gctcgagcac gtgttcctca agtcaccggc cgaggcgttt
     1201 cgaatcatga tggaggtgca ccggaacgga cgcggcgtcg gcggcgtgta ccctcacgag
     1261 atcgccgaga cgaagtccgc catggtgcag agcctcgccc gcgagcgggg cttcccgctg
     1321 cagacgagcg tcgaggaagc gtgatcgggc attctggagc gtgacatgtt cagcgccgcg
     1381 gtcgagatgc tcttgcacat cgcctaccgg gaagcggtct cccggcgcca cacgacactg
     1441 accctcgagc acctgctcta cgtcctgacc cacgatctgg agggcgagcg gatcctcgca
     1501 gcctgcggcg ccgacctgcc ccgcctgcgc cgcgagctgg acaccttcct ccagagttcg
     1561 gtcgagcagt tcccgcgcgg gatggagaag gagcccgagc agacccgcgc cttccggcgg
     1621 gtgctgcaga cggccgtcct gcacgtgcag agcgcgggca agcccgaggt gaacgcgggc
     1681 gacatcgttg cggcgatgct gcagcagacc aacgcctact cctgccagct gctcgaggcg
     1741 cagggggtga cccgtctcga cgtcctcaac tacatctcgc acggcatctc gaaagtcccc
     1801 gtgccgccga caccgggcga ggaagggcgc cggcgggggc agccccagcc cggggtcgag
     1861 gggcaggccg ggtcgtcgcg cgacccgttg gcgagctacg cggtgaacct gaccgagcgg
     1921 gcgcgtgccg gcctgctcga cccgctgatc gggcgttcgg tcgagctgca gcggaccatg
     1981 gagatcctct gccggcgtcg gaagaacaac ccggtcttcg tgggcgacgc cggggtgggc
     2041 aagacggcgc tggccgaggg gctcgcgatc cggctgctcg agtcgaccgt cccgccgatc
     2101 ctggaggggg ccgagatcta cagcctcgac acggcggcgc tgctggcggg tacgcggttc
     2161 cgcggcgact tcgaggagcg cttcaaggcg gtgctcgcgg cgctgaagtc gcggcggctg
     2221 ccgatcctct tcatcgacga gatccacggg acgatcggcg ccggggcgac caccgggggc
     2281 acgatggacc tggcgacgct gatcaagccg gtcctcaccg cgggcgagct gcgcgtggtc
     2341 ggttcgacca ccttcgagga gttcaagcag atcgagaagg accgggctct cgcgcggcgg
     2401 ctgcaaaagg tcgtcgtcga ggagccgagc gtcgaggaga ccatccgcat cctgcagggg
     2461 ctgcggagcc ggtacgagga gcaccaccag gtcgagtacg aggacgccgc cctcgaggcc
     2521 gcgaccaggc tcgccaagcg cttcctgagg gaataccggc tgcccgacag cgccatcgac
     2581 atcctggacg aggccggggc gatgctgcgg ctgcaggggc cggcggcccc cggcgggccg
     2641 ggggccgcgg aagcagcgcc gcggcgggtg accccggcgg agatcgagcg ggtggtgggc
     2701 cgcatggcgc ggatccccga caagcaggcg acctcctcgg agaaggagcg cctgcgcacg
     2761 ctcgaggcct cgctccgccg cgtcgtcttc ggccaggacg aggcggtccg catcgtcgcg
     2821 gcggcgatca agcgatcgcg cgccggcctc gcacagcccg accggccggc cggctgcttc
     2881 ctcttcacgg ggccgaccgg ggtgggcaag accgagctgg cgaggcagct cgcgctgcac
     2941 ctcggcaacg agttcctgcg ctacgacatg agcgagttca tggagaagca cgccgtgtcg
     3001 cggctgatcg gcgcgcctcc ggggtacgtg ggcttcgagc agggcggctt gctcgtggac
     3061 gccgtccgga cccatcccta cgccgtggtg ctgctcgacg agatcgagaa ggcgcacccc
     3121 gacatgttca acatcctgct gcaggtgatg gatcacgcga cgctgaccga caacaacggc
     3181 cgccgcgccg acttccggca ggtggtgctg atcatgacct cgaacgccgg ctcgcgcgag
     3241 atgagtgccg cctcgatcgg cttcgggggc ggcggcgcgt cggcggcgca ggaggccggg
     3301 ctgcgcgacc aggcctcgcg aagccgggcg aggaccgcca tcgagcgggt cttcagcccc
     3361 gagttccgga accggctcga cgcgacggtc accttcgcgg cgctcagccc ctcggtgatg
     3421 gagaccatcg tcgaaaagtt catcctgcag ctcgagtcgc agctctcgga gcggcggatc
     3481 gagatcgccc tcgagccggc ggcgcgggcg tggctggcgc ggcggggcta cgacccgatc
     3541 tacggcgcgc gccccctcgc gcgggtgatc caatccgagg tgcgggaccc gctgaccgac
     3601 gagatcctct tcgggcgtct cgagcacggc ggcacggtgc gaatcggcct ggaggacgat
     3661 cacctcacct tcgccttcgc cgaggccgca gcccagtaag agggctcagg gttcaaggct
     3721 catggctcaa ggccgatgaa cgacgacaag accaacggga aggaacgcga gccggttgac
     3781 ggcccggtga aggtcatcga ccggcggtgg tggacgcacg gcgaaaccgg cgccccggtc
     3841 gagccgcccc gcaagcccac ctacctcgag gaactcgaac ggcagatcgc cgagaaggac
     3901 caggccatcc aggcgtacgc ctcgaaatac cgcgagagcg cggccgagtt cgaccaggtg
     3961 cgcgcgcgcc tgcggcgcga tctcgaccgc gacatcgagc gggcgcgccg cgcggtgctc
     4021 gccgacttcc tcgagattgc cgacaacctc gatcgagcgc tggccgcggc gccggccggc
     4081 accgacgaag cgttcgtccg cggcgtggac ctggtgcgcc agctgctgct cgcgcgcttc
     4141 gccgcgcacg gcgtcacgcc gatggatgcc gaggggaaac gcttcgaccc ggcctggcac
     4201 gatgccgtgt cgctgatgga ggtggccgac cccgggcagg acgaggtcgt gcaggccgtg
     4261 gtgaagaaag gctacctggt gggcggcgag gtgcttcgcc cggcggccgt ggtggtgggc
     4321 aaatacgcgg gccagggaga ggctcctacc ccataggccc cagcccctgg cccccggccg
     4381 ctagctcaca accgtctgcg cggccgcctg cagccccgcg tcgagggcgg cgaagttcag
     4441 gtcgagcagc gccgccttct tcccggacag ctccttgcgc agcgccgcca acgcgctgtc
     4501 gcggccgacg atcggcacgc gcgcgatgag cgcaccgagc gcgacgacgt tggcgaggcg
     4561 ggggttcccc aggccgccgg cgatgcccgt gatcccgatg tacgccacct cgatgtcggt
     4621 tcgcgtgtgc gggatctcga tcagcgtccg gttgatcagc aggaagccgc cctcggccat
     4681 cttgggctcg aacttctcca gggaggggcg gttcatcgcc accaggacgt tcggccgcga
     4741 gacgaccggg gtcccgatgc gcttgtctga caccaccacc gagcagttcg cggtgcctcc
     4801 gcgcatctcg gggccgtagg aggggatcca cgacacctcc ttgccctcga tcatgccggc
     4861 ggtcgcaatc atcttgccca ggaacaggat tccctgcccg ccgaaccccg ccaggacgat
     4921 gtcgtaggtc ataacttcac ctcgccgggg acggcgtcac ggtaaacgcc gagcgggtac
     4981 tcgggcatca ggctcgactc gagccaatcc accgcttcgt tcggatcgac gccccagttg
     5041 gtggggcacg tggcgagcac ctccacgaag gtgaacccct cccccttctg ctggaggtcg
     5101 agggccttct tgatggcacg cttggtgcgc gtgaggttcg ccacgttgtt gagcgcggtc
     5161 cgctcgatgt agcgcggggc cttgagcgtc gcgagcatct cgctcacgtg cagcgggtgc
     5221 ccggcgaact tcgggtcgcg gccgtacggc gaggtggcgc ttgcctgccc caccagcgtg
     5281 gtcggcgcca tctgcccccc ggtcatcccg tagatcgcgt tgttgatgaa gaccaccgtg
     5341 aagttctcgc cccgggcggc cgcgtggatg atctcggccg tccccgacga agcgaggtct
     5401 ccgtcgccct ggtaggagat cacgatcgag tcggggttgg cccgcttcag cccggtgccg
     5461 acggctgggg cccgcccgtg cgcc
//
LOCUS       DAOPQX010000243         5480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16288_length_5480_cov_4.722581, whole genome shotgun sequence.
ACCESSION   DAOPQX010000243 DAOPQX010000000
VERSION     DAOPQX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16288_length_5480_cov_4.722581"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PKK95_12280"
     CDS             <1..829
                     /locus_tag="PKK95_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOC19044.1"
                     /translation="TIVSTLHATKPPAPDLAAPEPPRYDPAEIYGIVNADLRVPYDVR
                     EVIARLVDGSRFDEFKERYGTTLVTAFARLHGYLVGIVANNGVLFSESALKATHFIEL
                     CNLRGIPLVFLQNITGFMVGRDYERTGIARDGAKMVHAVANSVVPKFTVDIGGSFGAG
                     NYGMCGRAYDPRLLWMWPNARISVMGGEQAASVLASVKREQLAREGRTLTPEEEETIR
                     RPILEKYEHEGSPYYSTARIWDDGVLDPADTRDALGLALSAARNAPTGEPRFGVFRM"
     gene            834..1616
                     /locus_tag="PKK95_12285"
     CDS             834..1616
                     /locus_tag="PKK95_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOC19045.1"
                     /translation="MMFIRIERRGPVEYLTLNRPEVRNAFNGEMVRELRAWAEGVGAG
                     TGVRAVVLSGAGPSFCAGGDLEWMRKAAAFSREENLADAAAMAAMFGALDRLRVPLIG
                     RVHGAAMGGGVGLTAVCDIAVAAEDAQFALSEVRLGLVPSVIAPFVLAKIGRSAAREL
                     FLTGARFTARRALAIGLVHEVVPASSLDEEVARRVGAILAAGPEAVSAAKALIERVAG
                     AGPVQAAAITAEAIADRRASAEGQEGMRAFLEKRKPAWEEGR"
     gene            1617..3113
                     /locus_tag="PKK95_12290"
     CDS             1617..3113
                     /locus_tag="PKK95_12290"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOC19046.1"
                     /translation="MFRRVLVANRGEIALRVMRACRELGVETVAVYSDEDRSAPHAAA
                     ADRAVPIGSSSSYLDQAALLRAALDEGADAVHPGYGFLAENAGFAAACEQAGLAFIGP
                     PPRAIAEMGSKIASRAHAAAAGVMVVPGEAPGQEPSAIAAAAARLGYPVLVKASAGGG
                     GKGMRIVGDSSELARAVASARHEAGAAFGDATLYLERLLHRPRHVEVQVFGDTYGQVV
                     HLFERDCSIQRRHQKVVEESPSPGLPGALRERMGQAAVRLATHIGYRGAGTVEFLLEG
                     SGAAEPPFYFLEMNTRLQVEHPVTEAVVGVDLVQAQIAVAAGEPLPWSQERLTQRGHA
                     IECRIYAEDPAAGFLPQAGRLAVYREPVSPGIRIDSGVTEGTEIGVSYDPLLAKLVAC
                     AETREAARRRAVAALRQYAILGIRTNIPFLVRVLEHPRFRDATIDTGFLDEHDELATD
                     AQEPGALAAALAAAAVHEPERRPPEPGVAVAATADPWDLLSRWGRAWP"
     gene            3104..3619
                     /locus_tag="PKK95_12295"
     CDS             3104..3619
                     /locus_tag="PKK95_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOC19047.1"
                     /translation="MAVTPPGSRIGATILRLGEREFRVEVQPGLAVTIAPGEERAQVT
                     RLGADTYRVVTANRTWHVAVAASGSRLQVFADGNVFDLEIGSTPPMSRAGKRHDGDAL
                     AAPMPARVREVLVEVGQSVAAGDLLVTLEAMKMELAVRAPRDGRVVSIACRPGELVSQ
                     GVALLELEGTA"
     gene            3630..4580
                     /locus_tag="PKK95_12300"
     CDS             3630..4580
                     /locus_tag="PKK95_12300"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOC19048.1"
                     /translation="MPAQTAYPTAVRIVEVGPRDGLQNEAATLSTDEKVEFVERLAAS
                     GLPVVEAGAFVNPARVPQMADTAEVFGRVRRRAGTRYAALVPNIRGLERAMAAGADEV
                     AVFAAASETFSRRNINQGIAESLDNYASVCRTALDAGVPVRAYLSTAFGCPYEGPIDP
                     GRVAALCQQLRELGVFEVAVSDTIGIAHPGQVARVLDEVVPRIGVQAVALHFHDTRGM
                     ALANVLAALGYGVACFDSSAGGLGGCPFAPGASGNLATEDLVYMLDGLGIATAVRLGA
                     LLEASAFIASRVDHELPSRVYQAGRRTLHLQPEVTDAERG"
     gene            4564..4911
                     /locus_tag="PKK95_12305"
     CDS             4564..4911
                     /locus_tag="PKK95_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19049.1"
                     /translation="MPNADELMELARGCVDALDLSDRREVPSLADVEDQCEEQYGELL
                     DTLNEDEIFGAVDLERQQQILDTLESAAGEDKRELVRELADQHTRHVWLMQEAAFHVG
                     LALGLRMAGGRSQ"
     gene            5017..>5480
                     /locus_tag="PKK95_12310"
     CDS             5017..>5480
                     /locus_tag="PKK95_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOC19050.1"
                     /translation="MNTTAARRSPRPVRLAACLLAAVTAAWAGACGEGATTLAGPTAQ
                     KCQISVTAFTRAFGAAGGPGSVTLATARECSWSAASATQWISFPKQASGQGPASFGFA
                     VAANPAPRAREGTIEVSGQRLGISQEAAPCRFDLDSGTTRAGAPGGRASVRVS"
BASE COUNT          753 a         1926 c         2042 g          759 t
ORIGIN      
        1 gacgatcgtc tcgacgctcc acgcgacgaa accgccggcc cccgacctgg ccgccccgga
       61 accgccccgg tacgacccgg ccgagatcta cgggatcgtc aacgccgacc tgcgcgtgcc
      121 ctacgacgtg cgcgaggtga tcgcgcggct ggtggacggc tcgcgcttcg acgaattcaa
      181 ggagcgctac ggcaccacgc tcgtcaccgc cttcgcgcgc ctccacggct acctcgtcgg
      241 gatcgtggcg aacaacggcg tcctcttctc cgagtcggcg ctcaaagcca cgcacttcat
      301 cgagctgtgc aacctgcggg gcatcccgct ggtcttcctg cagaacatca ccgggttcat
      361 ggtgggccgc gactacgagc gaacggggat cgcgcgcgac ggcgcgaaga tggtgcacgc
      421 ggtcgccaac tcggtcgtcc cgaagttcac cgtcgacatc ggcggatcgt tcggcgccgg
      481 caactacggg atgtgcggcc gggcctacga cccgcggctg ctctggatgt ggcccaacgc
      541 ccgcatctcg gtgatgggcg gcgagcaggc ggcctcggtg ctggcctcgg tgaagcgcga
      601 gcagctggcg cgcgaggggc ggacgctcac acccgaggag gaggaaacga tcaggcggcc
      661 catcctcgag aagtacgaac acgaggggtc gccctactac tccaccgccc gcatctggga
      721 cgatggggtg ctcgacccgg ccgacacgcg cgacgccctc ggcctggcgc tgtcggcggc
      781 gcgcaacgcg ccaaccggcg agccgaggtt cggggtgttt cggatgtgat cgcatgatgt
      841 tcattcgaat cgagcgccgc ggccccgtcg agtacctcac cctcaaccgg cccgaggtgc
      901 gcaacgcctt caacggcgag atggttcgag agctgcgggc gtgggccgag ggcgttggcg
      961 cggggacggg cgtgcgcgcc gtcgttctgt cgggggccgg cccgtccttc tgcgccggcg
     1021 gcgacctcga gtggatgcgc aaggcggcgg ccttctcccg cgaggagaac ctggcggacg
     1081 cggccgcgat ggcggccatg ttcggcgcgc tcgaccgcct gagggtcccg ctcatcggcc
     1141 gcgtgcacgg cgccgcgatg ggcggcggcg tgggcctgac agccgtgtgc gacatcgcgg
     1201 tggcggccga ggacgcgcag ttcgccctgt cggaggtgcg gctcggcctg gtaccgtcgg
     1261 tcatcgcgcc gttcgtgctg gcgaagatcg ggaggtccgc cgcgcgagag ctgttcctca
     1321 ccggggcgcg gttcaccgcg cggcgcgcgc tcgcgatcgg cctggtgcac gaggtcgtgc
     1381 ccgcctcgtc gctcgacgaa gaggttgcgc gccgggtcgg ggcgatcctg gccgcggggc
     1441 ccgaagcggt ctcggccgcc aaggcgctga tcgagcgggt ggccggcgcc gggcccgtcc
     1501 aggccgccgc catcacggcc gaggcgatcg cagaccggcg cgcctcggcc gagggccagg
     1561 aaggcatgcg ggcgttcctc gagaagcgga agccggcctg ggaggagggg cgctaggtgt
     1621 tccgccgggt gctggtggcc aatcgcggcg agatcgcgct cagggtgatg cgggcctgcc
     1681 gcgagctggg cgtggagacg gtggccgtgt attcggacga ggaccgctcg gcgccgcacg
     1741 cggcggcggc agaccgcgcg gtcccgatcg gctcctcctc ctcgtacctc gaccaggccg
     1801 cgctcttgcg cgcggcactc gacgaggggg ccgacgcggt ccaccccggc tacggcttcc
     1861 ttgccgagaa tgcgggcttc gcggccgcgt gcgagcaggc cggcctggcc ttcatcggac
     1921 cgccgccgcg tgccatcgcg gagatgggct cgaagatcgc gtcgcgggcg cacgccgcgg
     1981 ccgccggcgt gatggtggtg cccggcgagg ctcccggcca ggagccgtcg gcgattgccg
     2041 cggccgccgc gcgcctcggc tacccggtgc tggtcaaggc atcggccgga ggcggcggca
     2101 agggcatgcg gatcgtcggc gattcgagcg agctggcgcg cgcggtcgca agcgcccggc
     2161 acgaggcggg cgcggcattc ggcgacgcaa ccctctacct ggagcggctg ctgcaccggc
     2221 cgcggcacgt cgaggtacag gtcttcggcg acacatacgg ccaggtcgtg cacctcttcg
     2281 agcgcgactg ctccatccag cggcggcacc agaaggtcgt cgaggagagc ccgtcgcccg
     2341 ggctgccggg agccttgcgc gagcggatgg ggcaagcggc cgtgcgcctc gcgacacaca
     2401 tcggctatcg tggcgccggc accgtcgagt tcctgctcga gggttccggc gcggccgagc
     2461 cccccttcta cttcctcgag atgaacaccc gcctgcaggt ggagcacccg gtgaccgagg
     2521 cggtcgtcgg ggtcgatctc gtgcaggcgc agatcgcggt cgcggccggg gagccgctcc
     2581 cgtggagcca ggagcggctg acccagcggg gtcatgcgat cgaatgccgc atctacgccg
     2641 aggatccggc cgccgggttc ctgccgcagg cgggacgcct ggcggtctac cgcgagccgg
     2701 tgtcgccggg catccggatc gacagcggcg tgaccgaggg aaccgaaatc ggcgtctcct
     2761 atgatcctct gctcgccaag ctcgtggcct gcgccgagac gcgcgaggcg gcgcgccgac
     2821 gggcggtcgc agcgctccgc cagtacgcga tcctcgggat ccggacgaac atcccgttcc
     2881 tggtgagggt gctcgagcac ccgcgcttcc gcgacgcgac gatcgacacc gggttcctcg
     2941 acgaacacga cgagctggcg acagacgccc aggagccagg cgccctcgcg gccgcgctgg
     3001 ccgcggccgc ggttcacgaa ccggagcgac gcccgcccga gcctggcgtt gcggtggcgg
     3061 ccacggcaga cccgtgggac ctcctctcgc gttggggacg cgcgtggccg tgacgccgcc
     3121 aggctcccgg atcggcgcga cgatcctgcg gctcggcgaa cgcgagttcc gcgtcgaggt
     3181 gcagcccggc ctcgccgtga cgatcgcgcc cggcgaggaa cgggcgcagg tcacccggct
     3241 cggggccgac acgtatcggg tcgtcaccgc gaaccgaacg tggcacgtgg ccgtggccgc
     3301 ctcgggcagc cggctgcagg tgttcgcgga cggcaacgtg ttcgacctcg agatcggtag
     3361 cacgccaccc atgtcacggg cgggaaagcg ccacgacggc gacgcgctgg cggcccccat
     3421 gccggcgcgg gtgcgcgagg tgctcgtgga ggtgggccaa tcggtggcgg cgggcgacct
     3481 gctcgtgacg ctcgaggcga tgaagatgga gctggccgtg cgcgcaccgc gcgacggccg
     3541 cgtggtgtcg attgcctgcc ggcccggcga gctggtctcg cagggcgtgg cgctgctgga
     3601 gctggaaggc acagcctgac ctcatcccca tgcctgcaca gactgcctat cccaccgccg
     3661 tccgcatcgt cgaggtgggg ccgagggacg gcctgcagaa cgaggcggcc accctgtcca
     3721 ccgacgagaa ggtcgagttc gtcgagcggc tcgcggcgtc cgggctgccc gtcgtcgagg
     3781 cgggggcctt cgtgaacccg gcccgcgtgc cgcagatggc cgacacggcc gaggtgttcg
     3841 gccgggtgag gcggcgagcc gggacgcggt atgcggccct cgtccccaac atcaggggcc
     3901 tcgagcgcgc gatggccgcg ggcgccgacg aggtggccgt cttcgccgcc gcgtccgaga
     3961 cgttcagccg ccgcaacatc aaccagggga tcgccgagtc gctcgacaac tacgcgtcgg
     4021 tctgccgaac cgcgctcgac gccggggtgc cggttcgcgc gtacctctcg accgcgttcg
     4081 ggtgtcccta cgagggcccg atcgaccccg gccgggtggc cgcgctctgc cagcaactgc
     4141 gcgagctggg cgtgttcgag gtggccgtca gcgacacgat cggcatcgcc caccccggcc
     4201 aggtggcgcg ggtgctcgac gaggtcgtcc cgagaatcgg cgtgcaggcg gtggcgctgc
     4261 acttccacga cacgcgcggg atggcgctgg caaacgtcct cgccgcgctc gggtacggtg
     4321 tggcctgctt cgactcctcg gccggcggcc tcggcgggtg tcccttcgcg ccgggagcga
     4381 gcggcaacct cgccaccgaa gatctcgtgt acatgctcga cgggctcggc atcgcgaccg
     4441 ctgtgcggct cggcgccctg ctcgaggctt cggccttcat cgcgtcgcgt gtggatcacg
     4501 agctgccgtc gcgcgtttac caggcgggcc ggcgcaccct tcatctccaa cccgaggtaa
     4561 ccgatgccga acgcggatga actgatggag ctggcccggg gctgcgtgga tgcgctcgac
     4621 ctctccgacc ggcgcgaagt accgtcgctg gccgacgtcg aggatcagtg cgaggagcag
     4681 tacggcgagc tgctcgacac gctgaacgag gacgagatct tcggcgcggt cgatctcgag
     4741 cgccagcagc agatcctcga cacgctcgaa tcggcagccg gtgaggacaa gcgtgagctg
     4801 gtccgggagc ttgccgacca gcacacgcgc cacgtgtggc tcatgcagga agcagcgttc
     4861 cacgtgggcc tcgccctcgg gctgcgcatg gccggcggcc gctcgcagta gcaggctccc
     4921 gctggcggtc agccgcatgt aacctgccga aacgcgaatc ggcgtgcccc gtgcatgagc
     4981 cgacggtgga gaggactgga cctttccatc ccggccatga acacgaccgc cgctcgccgc
     5041 tcgccccgac ctgttcgcct ggccgcgtgc ctcctcgctg ccgtcaccgc agcctgggcg
     5101 ggcgcgtgcg gggagggggc gactaccctg gccggtccga cggcacagaa gtgccagatc
     5161 tcggtaacgg ccttcacccg ggcgttcggc gcagccgggg gacccggcag cgtcacgctc
     5221 gcgaccgccc gcgagtgctc gtggagcgcc gcaagcgcca cacagtggat cagctttcca
     5281 aagcaggcct cgggacaggg accggcgtcg ttcggcttcg cggtggccgc caaccccgcg
     5341 ccgcgcgcgc gcgagggcac catcgaagtg tcggggcagc ggctcgggat ctcacaggag
     5401 gccgcgccct gccgcttcga tctcgattcg ggaactaccc gcgccggggc gccgggaggc
     5461 agggcatcgg tgcgcgtctc
//
LOCUS       DAOPQX010000244         5478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16296_length_5478_cov_3.975659, whole genome shotgun sequence.
ACCESSION   DAOPQX010000244 DAOPQX010000000
VERSION     DAOPQX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16296_length_5478_cov_3.975659"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PKK95_12315"
     CDS             <1..1300
                     /locus_tag="PKK95_12315"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOC19051.1"
                     /translation="VPAGHPLAAALSGDAARGPIRHVVFVVKENRTFDQVFGGRSGVD
                     GDPSLTTLGLGMNVKSKDGTRQVESAAVSPNHHALADRFAISDNFYCDADQSNTGHRW
                     VAGVYPNEWVEVNARSRIEARLFSPAPGRRSVAGSSAVVMPEDYNEAGALWEHLARHR
                     VPFFNFGFGTEMPGSIEEQAFKETGIRMAVGLPLPKPLFDRTSRKFATFNMAIPDQYR
                     MDAFEEELRDRWESGKEPFPALITLVLPNDHLTGEHPADGYPFAESYMADNDLALGRL
                     VQRLSRTRWWPNVLVIVTEDDPQGGVDHVEAHRSILMFIGPHVKRGYVSHALADFGSI
                     MRLIFTILGMPPLNQFDAAAPLPLDVFAAGVPPAEAAAPYDLRPPDRRVFDPDAALKP
                     FDRGFNWKLLAASPGLDDPEDMRRPFVDPDDALATVVTGK"
     gene            complement(1306..1770)
                     /locus_tag="PKK95_12320"
     CDS             complement(1306..1770)
                     /locus_tag="PKK95_12320"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOC19052.1"
                     /translation="MTVYLACTVRGDRSTLNIVRHVASRLEAAGHEVLTAHLLDDYVD
                     EAEGDGSNQAVFERDLRWLESCDLLVAEASGSSYGVGFEVGYILGRAATTGQRALVLY
                     EAAREPSVSRMISGNTHPACKVLAYGTTRQIDEFLTAHVAAVQYARRLALGR"
     gene            1857..2360
                     /locus_tag="PKK95_12325"
                     /pseudo
     CDS             1857..2360
                     /locus_tag="PKK95_12325"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2366..2788)
                     /locus_tag="PKK95_12330"
     CDS             complement(2366..2788)
                     /locus_tag="PKK95_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOC19053.1"
                     /translation="MKRDVRLHGLTSDHHHALVLALRIKEASKGGRIDAALVSQARER
                     YQQELAPHFAVEEEELLPALTARGRRDLVDRTLAEHEALRGHLAAAQAGEPGRLEAFG
                     LLLEAHVRFEEGELFPACEALVGDEVLARVAHRAPKGK"
     gene            complement(2799..3242)
                     /locus_tag="PKK95_12335"
     CDS             complement(2799..3242)
                     /locus_tag="PKK95_12335"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOC19054.1"
                     /translation="MKASVIRSYLEKFLREYLESDEPLEPDEDGDYCFRRGSAEILVS
                     LIEEEQSVVSILSVLLRGVKKSQKLLEALNDTNADCPFAKVYWREEGVVLRLDLLAES
                     LDDAQLRMACSLVSRLADELDTTLEKEFRGDLAFSDDSEEEAAKA"
     gene            3376..>5478
                     /locus_tag="PKK95_12340"
     CDS             3376..>5478
                     /locus_tag="PKK95_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOC19055.1"
                     /translation="MPRNTTPPQQQMKHDGALSFELDRRAFLKAAGSGLFILIPASSL
                     AALGQARGGQRSYPDDLNAYLRIGENGRVTCYSGKIEMGQANTTALAQMLAEELEVGL
                     DAIDMVMGDTRLCPWDGGTNGSRSIKYFGPALRAAGAEAREVLVQLAAERLKVPASRL
                     APRDGFVVDRRSSSTRVAYGSLVKGRRIERRLEKKPALKAPGGCKVMGTSPPPSNARD
                     KVTGKAVFAGDVQLAGMLHGRVLRPPVHGAQLKSVDVAPARKHPGARVFQEGNFIAVV
                     HPTTDGAAAALGAIAAEWSTPTATVDDTTILEHLKAHRAESRTVEERGDLVHAEGLST
                     LTFEGTYFTPYIAHAPIETHSAVAQVSAGEATLWVSTQRPFGVQVEVARAIGLPEERV
                     RVITPLVGGGFGGKSAGPQAVEAARLSKLAGAPVKVVWTREEEFFLDTFRPAAYVTIR
                     SGLNADNRIVSWDFHTRFAGDRSSEMIYDVPHVRTRATGSFGGGGPHPFGTGAWRGPG
                     SNTNIFARESHVDLMAARVGIDPVEFRLRNLADPRLVRVLKAAAGRFRWTSSRAPSGR
                     GQGVALLDYLNTCVAAMAEIAVDAKGSIRVKRVVMAQDMGQAVNPDEARFQMEGCILM
                     GISSVLSEEIHFEGGDVKERNFDGYEITRFSQVPEIETILVDNPELPPQGCGEPAITA
                     MAAVLANALFDVTGVRAIR"
BASE COUNT          837 a         1959 c         1895 g          787 t
ORIGIN      
        1 ggttcccgcc ggccacccgc tcgccgccgc cctttccggc gacgcggcgc gcggaccgat
       61 ccggcacgtc gtcttcgtgg tgaaggagaa ccggacgttc gaccaggtgt tcggcggacg
      121 ttcgggggtc gacggcgacc cgtcgctcac cacgctcggc ctcggcatga acgtgaagag
      181 caaggacggc acgcggcagg tcgagagcgc cgccgtctcc cccaaccacc acgcgctcgc
      241 cgaccggttc gcgatcagcg acaacttcta ctgtgacgcc gaccagtcga acaccggcca
      301 ccggtgggtc gccggcgtat accccaacga gtgggtcgag gtgaacgcgc ggtcgcggat
      361 cgaggcgcgc ttgttcagcc cggcgcccgg ccgccggtcg gtggccggca gcagcgccgt
      421 cgtgatgccc gaggactaca acgaggccgg cgcgctctgg gagcacctgg cccgccaccg
      481 cgtgcccttc ttcaacttcg ggttcggcac cgagatgccc ggctcgatcg aggaacaggc
      541 cttcaaggaa accggcatcc gcatggcggt cggcttgccg ctgccgaagc cgctcttcga
      601 ccgcacttcg cgcaagttcg ccaccttcaa catggccatc cccgaccagt accggatgga
      661 cgcgttcgag gaggaactgc gcgaccgctg ggagagcggg aaggagccgt tcccggccct
      721 gatcacgttg gtgctgccga acgaccacct gaccggcgag cacccggccg acggctaccc
      781 gttcgccgag tcgtacatgg ccgacaacga cctcgcgctc ggccgcctcg ttcaaaggct
      841 ctcgcgcacc cgctggtggc cgaacgtgct cgtcatcgtc accgaggacg acccgcaggg
      901 aggcgtcgac cacgtggagg cccaccggtc gatcctgatg ttcatcggcc cgcacgtgaa
      961 gcgcggctac gtctcgcacg cgctggccga cttcggctcg atcatgcggc tgatcttcac
     1021 gatcctcggg atgccgccgc tgaaccagtt cgacgcggcc gctccgctcc cgctcgacgt
     1081 cttcgccgcg ggcgtccccc cggcggaggc cgcggcgccg tacgacctgc ggccgcccga
     1141 ccgccgcgtc ttcgaccccg acgcggcgct gaagccgttc gaccgcgggt tcaactggaa
     1201 gctgctggcc gcctcgccgg gcctcgacga ccccgaggac atgcgccgcc cgttcgtaga
     1261 ccccgacgac gcgctggcga cggtggtaac gggaaagtag gacgatcagc ggccgagcgc
     1321 gaggcgccgc gcatattgga ccgccgcgac gtgagccgtc aggaactcgt cgatctgccg
     1381 ggtcgtcccg taggcgagca ccttgcacgc cgggtgcgtg ttgcccgaga tcatcctcga
     1441 cacgctcggc tcgcgcgccg cctcgtacaa caccagcgcg cgctgaccgg tggtggccgc
     1501 ccgccccagg atgtacccca cctcgaaccc caccccgtag ctcgagcccg acgcctcggc
     1561 caccagcagg tcgcacgact cgagccaccg caggtcgcgt tcgaagacgg cctggttcga
     1621 gccgtcgccc tcggcctcgt ccacgtagtc gtccaggagg tgagcggtca gcacctcgtg
     1681 ccccgcggcc tcgaggcgcg aggcgacgtg gcggacgatg ttgagcgtgg agcggtcgcc
     1741 gcgaacggtg caggccaggt agacggtcat gacgtggatt gtcgacgccc gccgggctgg
     1801 ccgtcaacgt gaccccagta gcacaggcgt cccgcctgtg aggggaggac gggttcgcga
     1861 ttgtcaccga gacggtcgtg ttccgtttcg agggtgagac cttcacccgc gtcacgcgcg
     1921 aggtgccgct gcggcgaacc gacggcatcg acatcatcga ggccggcctc gacgggatgg
     1981 tcgtcgggcg gggccgcgag gcaggcccca cgcgcctgga ggtgtcgcgc cacgagggag
     2041 acctgcgcat cgtctggcgc ttcccgccaa ccgaggggac gcaccgcttc accctgacct
     2101 acctcgtgcg cggactggtg gaacggaagc ctggcgagga cacggtcgcg tggacggcgc
     2161 ttccgcgcga ccacgagtac cgcatcgcca gcagccgcat cacgttccag tggccctcgt
     2221 ctgcgcgcct ccggcagctg gaggccggcg ggcattcggt ggccgccggc gagaacggcg
     2281 cggcgttcga cctgcagggc gagtccgggg tgggcggcgc aggcggcggg gctggcggcg
     2341 gctcgagcag cgcctcctga gtggcctact tacccttcgg cgcgcgatgg gccacgcgcg
     2401 ccagcacctc gtcgcccacg agcgcctcac aggcggggaa cagctctcct tcttcgaacc
     2461 tgacgtgcgc ctcgagaagg aggccgaacg cctcgaggcg gccgggctct cccgcttgcg
     2521 cggcggcaag atgtccgcgc agcgcctcgt gctccgcgag cgtccggtcc accaggtcgc
     2581 ggcggccgcg ggcggtgagc gccggaagca gctcctcctc ctccaccgcg aagtgcggcg
     2641 ccagctcctg ctggtagcgc tcccgggcct gtgagacgag ggcggcgtcg atgcggcctc
     2701 ccttcgaggc ctccttaatc ctcaaggcaa gcaccagcgc gtggtgatga tccgaggtca
     2761 ggccgtgaag gcggacgtcg cgcttcataa ggcaccagct acgccttggc cgcttcctcc
     2821 tcgctgtcgt cgctgaacgc caggtcgccc cggaactcct tctcgagcgt ggtgtcgagc
     2881 tcgtcggcaa ggcgcgagac caggctgcac gccatccgca gctgcgcatc gtccaggctc
     2941 tcggccagca ggtcgaggcg cagcacgacc ccctcctcgc gccagtacac cttcgcgaaa
     3001 gggcagtcgg cgttggtgtc gttgagcgcc tcgagcagct tctggctctt cttcacgccg
     3061 cgcagcagca ccgagaggat cgagacgacc gactgctctt cctcgatcag cgagacgagg
     3121 atctcggcgc tgccgcgccg gaaacagtag tcgccatcct cgtccggctc gagcggctcg
     3181 tcgctctcca ggtactcccg caggaacttc tcgaggtagg aacggatgac gctggccttc
     3241 aaaacaacct ccagacgggc gaggacggct gtcgcaccgt ggcgccccgc gtttcgccgc
     3301 gcacagtcta ccgcgcgcag tttaccttgt ttcgcgcccc cgggaggctt gggctcctgg
     3361 aggggcggtg ctaagatgcc gcggaacacc acaccgccac agcagcagat gaagcacgac
     3421 ggggcacttt ccttcgagct cgaccggcgc gccttcctga aggccgcggg ctcgggcctc
     3481 ttcatcctca tccctgcgag cagcctggcc gcgctcggcc aggcacgggg cggccagcgg
     3541 tcgtacccgg acgacctcaa cgcctacctg cgcatcggcg agaacgggcg cgtcacctgc
     3601 tactcgggca agatcgagat gggccaggcg aacaccaccg cgctggccca gatgctggcc
     3661 gaggagctgg aggtcgggct cgacgcgatc gacatggtga tgggcgacac gcggctctgc
     3721 ccgtgggacg gcggcaccaa cggctcgcgc agcatcaagt actttggccc cgcactcagg
     3781 gccgccggcg ccgaggcgcg cgaggtgctc gtccagcttg ccgccgaacg cctgaaggtg
     3841 ccggcctcgc gcctcgcgcc gcgcgacggt ttcgtggtcg accggcgatc gtcctcgacg
     3901 cgcgtcgcct acgggtcgct cgtgaagggc cggcgcatcg agcgccgcct cgagaagaag
     3961 cccgcgctca aggcgccggg cggctgcaag gtgatgggca cgtcaccgcc cccgtccaac
     4021 gcgcgcgaca aggtcaccgg caaggcggtg ttcgccggcg acgtccagct ggccggcatg
     4081 ctgcacggcc gcgttctccg cccgccggta cacggcgcgc agctgaagag cgtggacgtg
     4141 gcgccggccc gcaagcaccc gggggcgcgc gtcttccagg aaggaaactt catcgccgtc
     4201 gtccacccga ccaccgacgg cgccgccgcc gcgctcggcg cgattgcggc ggagtggtcc
     4261 acgcccaccg cgacggtaga cgacacgacg atcctcgagc acctgaaggc ccaccgcgca
     4321 gagtcgcgca ccgtcgagga gaggggcgac ctggtgcacg ccgagggcct ttcgacgctc
     4381 accttcgagg gaacgtactt caccccttac atcgcccacg cgccgatcga aacgcactcg
     4441 gccgtcgcgc aggtcagcgc cggcgaggcc accctctggg tgtcgacgca gcgcccgttc
     4501 ggggtccagg tcgaggtggc ccgcgccatc ggcctgcccg aggaacgggt gcgggtgatc
     4561 acgccgctcg tcggcggcgg gttcggcggc aagagcgccg ggccgcaggc cgtcgaggcc
     4621 gcgcggctgt cgaagctggc aggcgctccc gtaaaggttg tctggacgcg cgaggaggag
     4681 ttcttcctcg acaccttccg tccggcggcc tacgtcacga tccggtccgg cctcaacgcc
     4741 gacaaccgca tcgtcagctg ggacttccac acgcgcttcg ccggcgaccg cagctccgag
     4801 atgatctacg acgtgcccca cgtgcggacg cgcgccaccg gcagcttcgg cggcggcggg
     4861 ccgcatccct ttggcaccgg tgcctggcgc ggccctggaa gcaacacgaa catcttcgca
     4921 cgcgaatcgc acgtcgacct gatggccgcg cgggtcggca tcgaccccgt ggagttccgg
     4981 ctgaggaacc ttgcggatcc gaggctggtc agggtgctga aggcggcggc cggtcgcttc
     5041 aggtggacga gctcgcgggc gccgagcggg cgcggccagg gcgtcgcgct gctcgactac
     5101 ctgaacacgt gcgtggccgc gatggccgag atcgcggtgg acgcgaaggg gtcgatccgc
     5161 gtgaagcggg tggtgatggc ccaggatatg gggcaggccg tgaacccaga cgaggcgcgc
     5221 ttccagatgg agggatgcat cctcatgggg atctcgtcgg tgctgagcga ggagatccac
     5281 ttcgagggcg gcgacgtgaa ggagcggaac ttcgacggct acgagatcac ccgtttctcg
     5341 caggtgccgg agatagagac catcctggtg gacaatcccg aactgccgcc acagggctgc
     5401 ggcgagccgg ccatcaccgc gatggcggcc gtcctggcga atgcgctctt cgacgtcacc
     5461 ggcgtgcgtg cgatccgc
//
LOCUS       DAOPQX010000245         5471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16324_length_5471_cov_4.232275, whole genome shotgun sequence.
ACCESSION   DAOPQX010000245 DAOPQX010000000
VERSION     DAOPQX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16324_length_5471_cov_4.232275"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PKK95_12345"
     CDS             complement(<1..557)
                     /locus_tag="PKK95_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC19056.1"
                     /translation="MTLSTAHLNPRLLAAEYAVRGPIVQRAQELEAGGRKIIYCNIGN
                     PQALRQPPLTYIRQLICLLEYPELLQDDQVRARFPKDVVERARFVLSKHPHGTGAYTQ
                     SPGIPFIRQAVADFIARRDGVPADPRRVILTDGASKAAQAVLTALLRAPGDGFMIPVP
                     QYPLYSASLTLYGGERIGYFLDERNG"
     gene            complement(554..1987)
                     /locus_tag="PKK95_12350"
     CDS             complement(554..1987)
                     /locus_tag="PKK95_12350"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOC19057.1"
                     /translation="MSSWVTLLVDLSIIALLVAGIAQFRTPRGARTGNATAAVALLLA
                     ILLVLYRNAILSPGLLAAALVVGGAVGALVAMRVTMIQIPAMVAFQHGMGGVAASAIA
                     FVELTRAHTPLTVVGEVSGFLGLAIGAATFSGSMLASAKLAAKMKQTPTVLPGHNGLL
                     LVLLAAIAALCVYAAAASGLPLQLALAGVLAASVALGYVFAVRIGGADMPVLISFLNA
                     TAGLAAAFCGIVIQNRLLIACGATVAASGSILTAVMCKAMNRHFTKVLSGGKAAVAPA
                     AASAPKPAQAEKPVARTREDAMEEAKALLRESRSVIIIPGYGMALAGAQFEVAALARR
                     LEQMGKSVSFAVHPVAGRMPGHMNVLLAEADVDYDKLREMDEVNPAFPETDVVLIVGA
                     SDVVNPAAIEKPDTPISGMPILNAHEAKRALVCNLDERPGYSGVPNSLYDLPSTLLLL
                     GDAKATVTGLLQSLPAEAGVEVEEVMA"
     gene            complement(2019..2336)
                     /locus_tag="PKK95_12355"
     CDS             complement(2019..2336)
                     /locus_tag="PKK95_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOC19058.1"
                     /translation="MADLALMLLVFALSFGVGYVLISRVPPLLHTPLMSMTNAISAVT
                     ILGAILLFAVAMGPGEKVVGAIALATAAFNLVGGFVITDRMTGLFRTHHEPAGNGSRP
                     GNS"
     gene            complement(2361..3530)
                     /locus_tag="PKK95_12360"
     CDS             complement(2361..3530)
                     /locus_tag="PKK95_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOC19059.1"
                     /translation="MRVVIPKEILPAEKRVAAIPETVAKLRSKGFEVAVESGAGAGIF
                     ISDADYEKAGAVIEADVERLLAGADVILKVKQPIFNEAKKKHEVEMMPDGAILVTFLH
                     PAAPDSHDMVRMLRDRHITSFTMDGIPRTSRAQRMDALTSMSTVTGYRAVLIAAQHLP
                     RFVPMIGTAIGAVKPAHVLVVGAGVVGLQAIATAKRLGAVVKAWDIREAARTEAISLG
                     AKVVGFDVPQELAIAEGGYAKLLPAEWLEKEQKAIEDVVAESDMIILSALVPGALAPV
                     IVTRKAVGRMKPGSVIVDVSIDQGGNCELTKPGQEIVEHDVMVSGLQNIPGRMAMHAS
                     WLYAHNMFHYVDNLFKAVPGSPDLSDDIVAASLVTKGGQILFKPALEAMGEVVAV"
     gene            complement(3576..4358)
                     /locus_tag="PKK95_12365"
     CDS             complement(3576..4358)
                     /locus_tag="PKK95_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19060.1"
                     /translation="MNTRPVEAKVRSSGPDSTSLLVRARGVAIEAEFAELQPCEFPIW
                     ASAPVPVEIPAGGTKSFIVGPFSGAITQKAATGDFPNIVRFPVYRKQETVPLEAYEQF
                     GDRGPAQVLAKVPIVALATAVSGVVEDRLIQRHAVLPFWNDGSSVLVTLDWYGGQPAE
                     GTPNPEWHLNASAGLPARLGLERLREASQLPPGASHRRYQIHASPWVLAARLLQDKGQ
                     ELLHPVWQREWTFDHFDDFEHALALIRTGLVTEPAEAAMSVA"
     gene            complement(4457..4918)
                     /locus_tag="PKK95_12370"
     CDS             complement(4457..4918)
                     /locus_tag="PKK95_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19061.1"
                     /translation="MFCRIDYFSTRVGGEAGSACQLLARLAAQRVDLIAAVSAPDGPR
                     GVALHLFPADPVDLAKAARAERIALEGPRGAVLVQGSDHSGALAAVLSKLAAAGIEPS
                     SAFSMRDGRGRFGCVIPLDPRSLEAALKALRLEMPERSFEAERRMGSHVSG"
     gene            complement(5090..>5471)
                     /locus_tag="PKK95_12375"
     CDS             complement(5090..>5471)
                     /locus_tag="PKK95_12375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC19062.1"
                     /translation="AGASGYVLKDSAFTELAEAIRVVGQGGRFLSPEIVGVVVDDYLQ
                     RLTPAPGSAAARLSDREREVLQLMAEGHPTADIAERLHISRKTVETHRKSIMIKLELH
                     SVAELTKFAVREGITSLDQARHHE"
BASE COUNT          812 a         1973 c         1878 g          808 t
ORIGIN      
        1 ccgttccgct cgtcgaggaa gtagccgatg cgctcgcccc cgtagagggt gaggctggcg
       61 ctgtacagcg ggtactgcgg gacggggatc atgaagccgt cccccggggc gcgcagcagc
      121 gccgtcagca ccgcctgggc cgccttgctg gccccgtcgg tgaggatcac ccggcgcggg
      181 tcggccggga cgccgtcgcg gcgcgcgatg aagtcggcga ccgcctggcg gatgaacggg
      241 atccccgggc tctgcgtgta ggcgccggtg ccgtgcgggt gcttcgacag gacgaaccgc
      301 gcgcgctcga cgacgtcctt cgggaagcgg gcccgcacct ggtcgtcctg cagcagctcc
      361 gggtattcca gcaggcagat cagctgcctg atgtaggtga gcgggggctg cctgagcgcc
      421 tgcgggttgc cgatgttgca gtagatgatc ttccggccgc cggcctccag ctcctgggcc
      481 ctctggacga tgggcccgcg cacggcgtac tcggcagcga gcagcctcgg gttgaggtgc
      541 gcggtcgaga gcgtcatgcc atcacctcct ccacctcgac gcccgcctcg gccggcaggc
      601 tttggagcag gccggtcacg gtggccttcg catcacccag cagcagcagc gtggacggca
      661 ggtcgtagag gctgttgggc acgccggagt accccggccg ctcgtcgagg ttgcacacca
      721 gcgcccgctt cgcctcgtgg gcgttgagga tcggcatccc cgagatgggg gtgtcgggct
      781 tctcgattgc cgcggggttg acgacgtccg acgcgccgac gatcaggacg acgtcggtct
      841 cggggaacgc cgggttcacc tcgtccatct cgcgcagctt gtcgtagtcg acgtccgcct
      901 cggcgagcag gacgttcatg tgaccgggca tgcggcccgc cacggggtgg acggcgaagc
      961 tcacgctctt gcccatctgc tcgagccgcc tcgccagcgc cgcgacctcg aactgcgcgc
     1021 cggcgagcgc catgccgtac cccgggatga tgatgacgct gcgcgactcg cgcaggagcg
     1081 ccttcgcctc ctccatcgcg tcctcgcggg tcctggcgac cggcttctcc gcttgcgcgg
     1141 gcttcggggc cgacgccgcg gcgggcgcga ccgccgcctt gccgcccgag agcaccttgg
     1201 tgaagtgccg gttcatcgcc ttgcacatga ccgccgtcag gatcgagccg gacgccgcca
     1261 ccgtggcgcc gcacgcgatc aggaggcggt tctggatgac gatgccgcag aaggcggccg
     1321 ccaggccggc ggtcgcgttg aggaacgaga tcagcacggg catgtcggcg ccgccgatcc
     1381 gcacggcgaa gacgtacccg agcgccaccg acgcggcgag cacgccagcc agcgccagct
     1441 gcagcggcag gccggaagcg gcggccgcgt acacgcacag cgccgcgatc gcggcgagca
     1501 ggacgagcag cagtccgttg tgccccggca gcaccgtggg cgtctgcttc atcttggcgg
     1561 ccagcttggc gctggccagc atgctgccgc tgaaggtcgc ggcgccgatc gccaggccca
     1621 ggaaccccga cacctcgcca accacggtca ggggcgtgtg agcgcgcgtc agctccacga
     1681 acgcgatggc cgacgcggcg acgcctccca tcccgtgctg gaaggcaacc atggcgggga
     1741 tctggatcat ggtgacgcgc atcgccacca gcgccccgac ggcgccgccg acgaccaggg
     1801 ccgccgcgag caggcccggc gagaggatgg cgttgcggta gagcaccagc aggatcgcga
     1861 gcagcagcgc gacggccgcg gtcgcgttgc ccgtccgcgc gccgcgcggc gtccggaact
     1921 gcgcgatgcc ggccaccagc agcgcgatga tcgagagatc gacgagaagt gtcacccagc
     1981 tagacatgat cagctccaag agaggtggtc ctcctccgtc acgagttgcc cgggcggctg
     2041 ccgttcccgg ccggctcgtg gtgcgtcctg aacaggccgg tcatccggtc ggtgatgacg
     2101 aagccgccga ccaggttgaa ggccgccgtc gcgagcgcga tcgcgccgac gaccttctcc
     2161 cccggcccca tcgcgaccgc gaacaggagg atcgcgccga ggatcgtcac ggccgagatg
     2221 gcattggtca tcgacatcag cggcgtgtgc agcagcgggg gcacccgcga gatcaggacg
     2281 taccccacgc cgaacgacag ggcgaacacg agaagcatca atgccagatc ggccatgacg
     2341 agtcctcctc ctggctccgg ctagaccgcc acaacctcgc ccatggcctc gagcgccggc
     2401 ttgaagagaa tctgcccgcc cttggtcacc agcgatgccg cgacgatgtc gtcggaaagg
     2461 tccggggagc ccggaaccgc cttgaacagg ttgtcgacgt agtggaacat gttgtgggcg
     2521 tagagccagc tggcgtgcat cgccatccgg cccgggatgt tctgcagccc gctgaccatg
     2581 acgtcgtgct cgacgatctc ctgccccggc ttggtcagct cgcagttgcc gccctggtcg
     2641 atcgacacgt ccacgatcac cgaccccggc ttcatgcggc cgacggcctt ccgggtgacg
     2701 atgaccggcg ccagcgcccc gggcaccagg gcggacagga tgatcatgtc cgactcggcc
     2761 accacgtcct cgatcgcctt ctgctccttc tcgagccact cggcgggcag cagcttcgcg
     2821 tagccgccct cggcgatggc cagctcctgc gggacgtcga agccgacgac cttggctccc
     2881 aggctgatcg cctcggtgcg cgccgcctcg cggatgtccc acgccttgac gaccgccccg
     2941 aggcgcttcg cggtggcaat cgcctgcagc ccgaccacgc ccgcgcccac caccagcacg
     3001 tgcgccggct tcaccgcgcc gatcgcggtg ccgatcatcg gcacgaagcg cgggaggtgc
     3061 tgggcggcga tcaggaccgc gcggtacccg gtgaccgtgc tcatcgaggt cagcgcgtcc
     3121 atccgctgcg cgcgcgaggt ccgcgggatc ccgtccatcg tgaacgaggt gatgtgacgg
     3181 tcgcgcagca tgcgcaccat gtcgtggctg tcgggcgcag ccgggtggag gaaggtgacg
     3241 aggatggcgc cgtcgggcat catctccacc tcgtgcttct tcttggcctc gttgaagatc
     3301 ggctgcttga ccttcaggat gacgtcggcg cccgccagca ggcgctcgac gtcggcctcg
     3361 atcacggccc cggccttctc gtagtcggcg tccgagatga agattcccgc gccggcgccc
     3421 gactccacgg ccacctcgaa ccccttgctg cgcaacttcg ccacggtctc ggggatcgcc
     3481 gcgacgcgct tctcggcggg caggatctcc ttcgggataa ccactctcat gacgtcagct
     3541 ccttgtctgc aagatgagtc cacttccccg tgccttcacg cgacgctcat cgccgcctcg
     3601 gcgggctcgg tcaccagccc cgtcctgatg agggcgagcg cgtgctcgaa atcatcgaag
     3661 tgatcgaagg tccactcgcg ctgccagacc gggtgcagca gctcctgccc cttgtcctgc
     3721 agcaggcggg ccgccagcac ccacgggctg gcgtggatct ggtaccggcg gtggctcgcg
     3781 ccgggcggca gttgcgacgc ctcgcgcagc cgctcgagcc ccagacgcgc cgggaggccg
     3841 gccgaggcgt tcaggtgcca ctcggggttc ggcgtgccct ccgccggctg ccctccgtac
     3901 cagtcgagcg tcaccagcac cgacgacccg tcgttccaga acgggagcac ggcgtgccgc
     3961 tggatcaggc ggtcttcgac gacccccgac acggccgtcg ccagcgcgac gatcggaacc
     4021 ttggccagga cctgcgccgg gccccgatcg ccgaactgct cgtacgcctc gagcggcacc
     4081 gtctcctgct tccggtacac cgggaaccgc acgatgttcg ggaagtcgcc cgtggccgcc
     4141 ttctgcgtga tcgcccccga gaacggcccg acgatgaagc tcttcgtccc gcccgccggg
     4201 atctccaccg gcaccggcgc actggcccag atcgggaact cgcacggctg caactcggcg
     4261 aactccgcct cgatcgccac gcctcgtgcc ctgaccagga gactcgttga gtcagggccg
     4321 ctcgagcgaa ccttcgcttc gactggtctg gtgttcatag ctcctcttcc tccgatgctc
     4381 gttctctctc cgagggtcca ctctacgggg gcgcgccggc gccgggaatc gggcgaaccc
     4441 ggattccacg ggtcgctcag cctgaaacgt ggcttcccat ccggcgctcc gcctcgaacg
     4501 atcgttccgg catctccagc ctgagcgcct tcaacgcggc ctcgagcgag cgcgggtcga
     4561 gcgggatgac gcacccgaag cgcccgcggc cgtcgcgcat cgagaaggcc gacgagggct
     4621 cgatgcccgc ggcggcaagc ttcgagagca ccgccgcgag cgctcccgag tggtcgctcc
     4681 cctgcacgag gaccgccccg cggggcccct cgagcgcaat ccgctccgcc cgcgccgcct
     4741 tcgcgagatc gacgggatcg gccggaaaca ggtgcagggc cacgccgcgc gggccgtcgg
     4801 gcgccgacac ggccgcgatc aggtccaccc gctgggcggc caggcgcgcc agcagttgac
     4861 aggccgagcc cgcctcgccg ccgacacgcg tggagaagta gtcgatccgg cagaacatct
     4921 cccctccaag cgccgctacg ccggaaatgg tatggagagc ggatgcggga cgtaatcggg
     4981 cgggaccggg ttgtggcgcg cgcgttggag gattcgccgg cggcccgaaa cggccgctgg
     5041 aggagggaaa gccggagcgc cctcgggcaa tcccctaagg gcgctgcccc tactcgtggt
     5101 ggcgggcctg gtcgagcgag gtgatgccct cgcgcacggc gaacttcgtc agctcggcga
     5161 cgctgtgcag ctcgagcttg atcatgatgc tcttgcggtg ggtctcgacc gtcttccggc
     5221 tgatgtgaag gcgttcggcg atgtcggcgg tgggatgccc ctcggccatc agctgcagga
     5281 cctcgcgctc gcggtccgag agccgcgccg ccgccgaccc gggcgccggg gtcagccgct
     5341 ggaggtagtc gtccaccacg accccgacga tctccgggct caggaagcgg ccgccctgcc
     5401 ccacgactcg aatcgcctcg gccagctcgg tgaacgcgct gtccttgagc acgtagcccg
     5461 aagcgccggc c
//
LOCUS       DAOPQX010000246         5463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16354_length_5463_cov_4.589867, whole genome shotgun sequence.
ACCESSION   DAOPQX010000246 DAOPQX010000000
VERSION     DAOPQX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16354_length_5463_cov_4.589867"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKK95_12380"
     CDS             <1..396
                     /locus_tag="PKK95_12380"
                     /inference="COORDINATES: protein motif:HMM:NF023762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOC19063.1"
                     /translation="GRAARNVNGRVLMYADVVTDSMRQAIAETDRRRRAQEAYNREHG
                     ITPASIVKQIDDVLASVYERDYAPVPAADEAPRWRSQAEFDAEVERLEREMRAAAANL
                     EFERAARLRDQLKAIRRRGMGLAAAAGGS"
     gene            404..1171
                     /locus_tag="PKK95_12385"
     CDS             404..1171
                     /locus_tag="PKK95_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC19064.1"
                     /translation="MLLERWTKASLADLQGYVLLVGRTLRGAVSRPFFVHDVVEQFEQ
                     IGVGSLTVVLLTGFFTGAVLALQSGITLDQFGARSLVGRLVGASMILELGPVLTALMI
                     TGRVGSSIAAELGSMEVTEQISALRALGADPVRKLVVPRVLAGLFMVPVLTVIADFVG
                     IAGGWVIATFQMGVASGVYWSSVTEGLFWQDPWVGLTKPFFLGFVIVTIACHVGLNVS
                     GGTQGVGRATTRAVVRASIAVLAVDFFLTKLMVFLFY"
     gene            1270..2154
                     /locus_tag="PKK95_12390"
     CDS             1270..2154
                     /locus_tag="PKK95_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC19065.1"
                     /translation="MRAERKGADGVMEQVPASESTVTLEEAFGPRAPVVLFDRVRLSF
                     DETEVLSDITFTLLKGHTKLLLGASGSGKSTILKILLGLLKPDAGIVWVNGHRIESLP
                     EERMMPLRNDMGMVFQEGALFDSLTVAENVGFKLYEQTRMPLDEVRARVEEVLGFIGL
                     SEYIDRMPSELSGGQRRRVAIARAMATKPRLLLYDEPTTGLDPITATTVDDEIIKLRD
                     LEEVSSIMVTHQLRDAFYIATHSARREDGRITIVPADEAKVREAEFIMLRDGRIVFEG
                     DAEALRTSKDEYLRTFLS"
     gene            2165..3205
                     /locus_tag="PKK95_12395"
     CDS             2165..3205
                     /locus_tag="PKK95_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOC19066.1"
                     /translation="MPRTRSLAFAQLKIGILAAVALAIAATVIFVLNGQGGFFWQRYP
                     LKARFPDVAGLKTGAPVRVAGMEVGTVQNIEFAGDLVEVTFEVSKEMRSRITTQSVAA
                     LGSLSLLGQTTVDISPSTRGEPLADWAYVPSGRLPGQLTDVAADASQALAELQRLLRD
                     VRAGKGTMGRLFTDERLFSEIQGFIHAAEQLARGLDRGEGTLGALLRDPSAYRSLESS
                     LGRLNAMLRRIEAGEGSLGRLVNDEQLARSLAGAGENMATITGRLARGEGSAGRLLHD
                     EALYSRLSGTAERLEQLTTRLAEGQGTAGQLLQDRQLYENMNGAASELRELVAAIKQD
                     PRKYLTVKVSVF"
     gene            3320..3586
                     /locus_tag="PKK95_12400"
     CDS             3320..3586
                     /locus_tag="PKK95_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOC19067.1"
                     /translation="MENGGEGKVLTAFLLGALTGAAVALLFAPASGQQTREFLGEKAR
                     EGRGKAEEAARHTREALSRQRETLSAAIDRGREAYREARDKETS"
     gene            3583..4014
                     /locus_tag="PKK95_12405"
     CDS             3583..4014
                     /locus_tag="PKK95_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19068.1"
                     /translation="MTGGGALPVVLLAIIAGATLVMALVQLAVVVFAARVALRAQAAA
                     ARLEQEVRPVIANAASAAEHASRAAALAAAQVERADQALGHLASRVDETTLTLQRALL
                     TPAREGRAVLAGVQAALAALLDRRALPAHRAGHDDDPLFIG"
     gene            4125..4676
                     /locus_tag="PKK95_12410"
     CDS             4125..4676
                     /locus_tag="PKK95_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19069.1"
                     /translation="MRTAVRRLVLLAVAGAVMVSMAGPAFAQEAKSVALVKELAAAMD
                     AAKLDAIAAKDPASQDTYFAALYFAGSQLLAVSARYEVPVLLDERIEKQEFREVYTDL
                     NSACMQGTKYLVMDIGADGLKPRKEDSRFDTYDTGSRSYAFDGEWRAQKLASEAEYDK
                     AFADADAHYAKILTALIAQVKKR"
     gene            complement(4919..4994)
                     /locus_tag="PKK95_12415"
     tRNA            complement(4919..4994)
                     /locus_tag="PKK95_12415"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4959..4961),aa:Val,seq:cac)
     gene            complement(5077..>5463)
                     /locus_tag="PKK95_12420"
     CDS             complement(5077..>5463)
                     /locus_tag="PKK95_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOC19070.1"
                     /translation="AGRPIPGFGHRIHRSDPRAARLLTMAHELELDGEHVRMIRTMER
                     VVEAQPPGAHGALPLNVDGAIAAVCADMGFEPELGNAMFMMARVPGLIAHAHEEQARE
                     APMRAIDPQGWVYDGPRTRRLPETRK"
BASE COUNT          803 a         1841 c         2003 g          816 t
ORIGIN      
        1 ggccgggcgg cccgcaacgt caacggccgg gtgctgatgt acgccgacgt ggtcaccgac
       61 tcgatgcggc aggcgatcgc cgaaaccgat cggcggcgcc gcgcccagga ggcgtacaat
      121 cgggagcacg ggatcacgcc cgcctcgatc gtcaagcaga tcgacgacgt gctggcgagc
      181 gtgtacgagc gggactacgc cccggtgccg gcggccgacg aggcgccgcg ctggcgctcc
      241 caggcggagt tcgacgcgga ggtcgagcgc ctcgagcgcg agatgcgggc ggcggcggcc
      301 aacctcgagt tcgagcgcgc ggcccggctc cgcgaccagc tgaaggcgat caggcggcgc
      361 gggatggggc tggccgccgc ggccgggggc tcgtgacgtg ttgatgctgc tcgaacgctg
      421 gaccaaggcg tcgctggccg acctgcaggg gtacgtcctg ctggttgggc gaacgctccg
      481 cggcgcggtg tcccgcccct tcttcgtgca cgacgtcgtc gagcagttcg agcagatcgg
      541 cgtcggatcg ctgaccgtcg tgctgctgac gggcttcttc accggcgccg tcctggcgct
      601 gcagtccggg atcaccctcg accagttcgg ggcccggtcg ctggtcggcc gcctggtcgg
      661 cgcgtcgatg atcctggagc tggggccggt tctgaccgcg ctgatgatca ccggccgggt
      721 gggttcgagc atcgccgccg agctcgggtc gatggaggtg accgagcaga tcagcgccct
      781 tcgggcgctc ggcgccgacc cggtccgcaa gctcgtcgtg ccgcgcgtcc tcgccggcct
      841 cttcatggtg ccggtgctga ccgtcatcgc cgacttcgtc ggcatcgcgg gcggctgggt
      901 gatcgccacc ttccagatgg gtgtggcgtc gggcgtctac tggagctcgg tgaccgaggg
      961 gctgttctgg caggacccct gggtcggcct gaccaagccg ttcttccttg gcttcgtgat
     1021 tgtgaccatc gcgtgccacg tggggctgaa cgtctcgggc ggtacgcagg gtgtcggcag
     1081 ggcgacgacc agggcggtgg tgcgcgcctc gatcgccgtc ctcgccgtgg acttcttcct
     1141 gaccaagctg atggtgttcc tgttctactg accccggagc tcacatgggt tgaccatgtt
     1201 cgctccggca gaaacggggc tgaagccccg tgctacggcg ctgcgcatgg tcaactgcgg
     1261 tgagcgggca tgagggctga gcgcaagggg gcggacggtg tgatggagca ggttccagcg
     1321 agcgagagca cggtgacgct ggaggaggcg ttcgggccgc gggcgcccgt ggtgctgttc
     1381 gaccgggtgc gcctgtcgtt cgacgagacg gaggtcctct ccgacatcac cttcacgctg
     1441 ctgaaggggc acaccaagct gctgctcggc gcgagcgggt cgggcaagag caccatcctc
     1501 aagatcctcc tcggcctgct gaagccggac gccgggatcg tgtgggtgaa cggccaccgc
     1561 atcgagagcc tgccggaaga gcggatgatg ccgctcagga acgacatggg gatggtgttc
     1621 caggagggag cgctgttcga ctcgctgacg gtggcggaga acgtgggctt caagctgtac
     1681 gagcagacgc gcatgccgct cgacgaggtg cgggcgcgcg tcgaggaggt gctcgggttc
     1741 atcggcctct ccgagtacat cgaccggatg ccgtcggagc tctccggcgg ccagcgccgg
     1801 cgggtggcca tcgcgcgcgc catggcgacc aagccgcgcc tgctgctcta cgacgagccg
     1861 accacggggc tcgacccgat caccgcgacc accgtggacg acgagatcat caagctgcgc
     1921 gacctggagg aggtctcgtc gatcatggtc acgcaccaac tgcgcgacgc tttctacatc
     1981 gcgacgcaca gcgcccgccg cgaggacggc cggatcacga tcgtccccgc cgacgaggcg
     2041 aaggtgcgag aggccgagtt catcatgctg agggacggcc ggatcgtctt cgagggcgac
     2101 gccgaggcgc tgcgcacctc gaaggacgag tatctcagga cgttcctgtc gtaggacgaa
     2161 ggccatgcca cgcacccgtt cgctcgcgtt cgcccagctg aagatcggca tcctcgcggc
     2221 cgtggcgctc gccatcgccg cgacggtgat cttcgtgctc aacggccagg gcggcttctt
     2281 ctggcagcgc tacccgctca aggcccgctt cccggacgtc gccggcctga agaccggcgc
     2341 gccggtgcgt gtcgccggca tggaggtggg caccgtccag aacatcgagt tcgccggcga
     2401 cctcgtggag gtcaccttcg aggtctccaa ggagatgcga agccggatca ccacccagtc
     2461 ggtcgccgcc ctcggttcgc tgagcctgct tggccagacg accgtggaca tcagtccctc
     2521 cacccggggc gagcccctcg ccgactgggc gtacgtgccg agcggcaggc tgcccgggca
     2581 gctcaccgac gtcgccgcgg acgcaagcca ggccctggcc gagctgcagc gcctgctccg
     2641 cgacgtgcgg gccggcaagg gcaccatggg gcggttgttc accgacgagc gcctgttcag
     2701 cgaaatccag ggtttcatcc acgccgccga gcagctggcc cgcggcctgg atcgcggcga
     2761 ggggaccctc ggcgcgctgc tccgcgaccc gtcggcttac cgctcgctcg aatcgtcgct
     2821 cggccgcctg aacgccatgc tccggcggat cgaggcgggg gaggggagcc tggggcggct
     2881 ggtgaacgac gagcagctgg cgcgctcgct cgcgggcgcc ggcgagaaca tggcgacaat
     2941 caccgggcgc ctcgcgcgtg gcgaggggtc ggcggggcgg ctgctgcacg acgaggcgct
     3001 gtacagccgg ttgtcgggga cggccgagcg cctggagcag ctgacgacgc ggctggccga
     3061 ggggcaggga acggcgggac agttgctgca agatcggcag ttgtatgaga acatgaacgg
     3121 ggcggcgagc gagttgcgcg agctggtggc cgccatcaag caggatcccc gcaaatacct
     3181 gaccgtgaag gtgagcgtgt tctgaccggg cgccggtgga gcagtcctcg cggctgctcg
     3241 tctcgagcgg gccgaacccc gctccacctg ggggacgggc cgggccggtg tggcggcgcg
     3301 agccgccggg gaggcgacga tggagaacgg cggtgaaggg aaggttctga cggcgtttct
     3361 gctgggggcg ttgacggggg cggccgtcgc gctgttgttc gcccccgcct cggggcagca
     3421 gacccgcgag ttcctcggcg aaaaggcgcg cgagggccgc ggcaaggccg aggaggccgc
     3481 tcggcacacg cgcgaggccc tgtcgcggca gcgcgagacg ctcagcgccg cgatcgaccg
     3541 cgggcgcgag gcgtaccgcg aggcccggga caaggagacc tcgtgacggg agggggagcc
     3601 ctcccggtcg tcctgcttgc gatcatcgcc ggcgcgacgc tggtcatggc gctcgtgcag
     3661 ctggcggtcg tcgtcttcgc cgcgcgggtt gccttgcgcg cccaggcggc ggccgcgcgg
     3721 ctggagcagg aggtgcgacc cgtgatcgcc aacgcggcat cggcggccga gcacgcctcg
     3781 cgggcggcgg cgctcgccgc cgcacaggtc gagcgggccg accaggcgct cggccacctc
     3841 gcctcgcggg tcgacgaaac gacgctgacc ctgcagcgcg cgctcctcac ccccgcccgc
     3901 gaggggcgcg cggtgctggc cggcgttcag gccgccctgg ccgccctgct cgaccggcgg
     3961 gccctgccgg cgcaccgcgc ggggcacgac gacgacccgt tgttcatcgg gtgaccccgt
     4021 cccgacttcc tcgcaaaacg gcgcattgac gccggaaggc tgaggtggta gaacacagcg
     4081 cacggagtcg gaaacgtgcc ctattcagga tcgggggatg atctatgaga accgcggttc
     4141 gccggctcgt cctgttggcg gtggccggtg cggtgatggt ctcgatggcc ggcccggcgt
     4201 tcgcgcagga agccaaatcc gtcgccctgg tgaaggaact cgccgcggcg atggacgcgg
     4261 cgaagctcga cgcgattgcc gccaaggacc cggcgtccca ggacacctac ttcgcggccc
     4321 tctatttcgc ggggagccag ctgctcgccg tcagcgcgcg gtacgaggtc cccgtgctgc
     4381 tcgatgagcg gatcgagaag caggagtttc gcgaggtgta cacggacctc aacagcgcgt
     4441 gcatgcaggg gacgaagtat ctcgtgatgg acatcggggc cgacggcctc aagcctcgca
     4501 aggaagacag ccgcttcgac acttacgaca cgggctcgag gagctacgcc ttcgacggcg
     4561 agtggcgggc ccagaagctg gcgagcgagg ccgagtacga caaggcgttc gccgacgccg
     4621 acgcgcacta cgccaagatc ctcacggccc tgatcgccca ggtgaagaag cggtagccgg
     4681 ccgcgggccg agagaagcac ggaggtggag aaccgggggc gccccttcgt tctccgcccg
     4741 gtcgagcccc ccgggtttca gcctcgaggc tgccgccatc cccacgttcg gccgccccct
     4801 tcctcgcaga gacgttcttc gcgcctcgcc actcgcgctt cagcgccagc gtagtcgcgt
     4861 ggccaggcac ggggcgcccg tccgcccgat tatcgatgcc gaggttgtcg aaggtgggtg
     4921 gtgggcacta gtggattcga accactggcc tcctgcgtgt gaagcaggcg atctaaccgc
     4981 tgatctaagt gcccacccga gaaagacccc ttagtgtaaa ccaactcgca cgtccacgac
     5041 aatcgctcgt ccgctcgccc ggcgtcctcc tgcggcctac ttccgggtct ccggcagcct
     5101 tcgcgtccgc gggccgtcgt agacccagcc ctgcggatcg atcgcccgca ttggcgcctc
     5161 gcgcgcctgc tcttcgtggg cgtgcgcgat gagaccgggg acccgcgcca tcatgaacat
     5221 ggcgttaccc agctccggct cgaagcccat gtcggcgcag accgcggcga tggccccgtc
     5281 cacgttgagc ggaagcgccc cgtgcgctcc cggcggctgc gcctccacga cccgctccat
     5341 cgtgcggatc atccgcacgt gctcgccgtc cagctcgagc tcgtgcgcca tcgtgagcaa
     5401 ccgtgccgct cgcgggtcgg agcggtggat gcggtgcccg aacccgggga tgggacggcc
     5461 ggc
//
LOCUS       DAOPQX010000247         5463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16355_length_5463_cov_4.379253, whole genome shotgun sequence.
ACCESSION   DAOPQX010000247 DAOPQX010000000
VERSION     DAOPQX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16355_length_5463_cov_4.379253"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2190
                     /locus_tag="PKK95_12425"
     CDS             <1..2190
                     /locus_tag="PKK95_12425"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOC19071.1"
                     /translation="DNRYQLWVNGTRVVFGPARGDLNHWRFETIDLAPHLHAGRNVLA
                     AVVWNYGELAPEAQTTWRTGFLIQGDTAAEAAVNSGTTWKAAANPAYSPIPFTHAQMR
                     GYFVVGPGERVDGAKFPWGWETEGYDDSAWSRAEPAVPDGRAHGSPRPSQDAGNRWLL
                     VPRSIPLMEESLERLAAVRQAAAVTAPEAFPRSEAPFTIPPRTEARLLLDNARLTTAY
                     PVLRYSGGAGARIRMGYAESLYLPRAKGGDKGNRNEVEGKEFVGNYDEVIADGGRDRT
                     YSPLWWRTYRYLELTVATRDEALVLHDLHGLYTGYPFERRQRLEAAPPVVSQILDVGW
                     RTARLCAHESYMDCPYYEQLQYAGDTRIQALVSLYASGDSRLVKNAISHLNDSRTAEG
                     ATMSRAPTRQQQYIPPFSLWWIGMVHDYYMYVDDPAFVREMLPGVRAVLQFFAAHQRP
                     NGSLGLMPWWNYVDWTTEWDGGVPPRDANGASAPQDLQLLLALDWASRLEEALGSRAI
                     AAEYRESAARLRGSIRTLYWDAGRRLFADTPDRKQYSQHANVLAVLAGVASGEEARDL
                     VTRVIADETLTQCSYYFRHYLHSAVNLVGEGDRYLGLLGEWKSMLARGLTTFAERYER
                     PGNPSRSDCHAWSSSPNFEIFRTVLGIDSAAPGFARVQVRPFVGSLDRVSGSIPHPKG
                     EISVRLERAGRGLRVEVVLPAGVPGEFSWGGTRRELAPGSNAFTVER"
     gene            2300..3727
                     /locus_tag="PKK95_12430"
     CDS             2300..3727
                     /locus_tag="PKK95_12430"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC19072.1"
                     /translation="MSARLRAGAAVSAAALLLGLQVAAGASAGTTQEAPPSGGRLDVP
                     VDYYKLPNGLKVVLSRDTTTPTAVVTIYYNIGFRNEPKDRTGFAHLFEHMMFQGSANL
                     GKMEFIRLIESNGGLLNGSTRFDFTNYYQVVPSHVLETVLWAEADRMRGLDITGENLK
                     NQQDVVKNEVKVNVLNQPYGGFPWIDLPMAANENWYNAHNFYGDLEHLDAATLEDVQS
                     FFRTYYAPNNAALVVAGDFDPARAKEWIERYFGDIPAAKQPPAPDLREPRQEKEKRAG
                     RTDPLAARPALGIAYHVPDRLTPQWYAFGLLDQVLAQGADSLLYDELVRKQGLTGDLS
                     AGINWGLGGMFDYSGPMLWMAQVFHDAGTPSEKVIAAIDTVVERLRSAPLDEATLERA
                     RVKMRSSLYGDLESFAGFGRANVLASFALFDDDPGRINRLEAEFAKVTPALVHETARE
                     FLRPGNRTIYTIEPGKAAPAKGGAR"
     gene            3724..5148
                     /locus_tag="PKK95_12435"
     CDS             3724..5148
                     /locus_tag="PKK95_12435"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOC19073.1"
                     /translation="MSRRTTALAVTAALLLLAPGVPGAREQQAPPASPQKQAPPPTGT
                     PKDFALPKPSRFTLPNGMAVTMVPFGQVPKVTLRLVVAAGNLYEGKDEVWLADLTGRM
                     LREGTAARPAAEVAREFAGMGGELSVGVGSDNASLSAEVLAERGADAARLIAEVARQP
                     LLPAGELARVKAGLTRDLAIQRSTPQAQAQERFHESIYGDHPYGRLFPTEEMLNGYTI
                     ERVRTFHRARYTPAASRLYVAGVFDAAALEGAIRDAFGAWKGSAGASEVQPPTAKAER
                     RFALLDRPGAPQSTLYLGLRVPDPSSADWVALQVTNSLLGGSFGSRITTNIREQKGYT
                     YSPFSAVNPHRRDAYWAEVADVTSADTGAALKEIFSEIERLRRDPPPEGELRGIKNNM
                     AGSFVVRNSSRQGVISQLAFVDEHGLGDGYLAGYVKRVMAVTPQEVTRVAREYLVPDR
                     MTLVVVGDRKTVEGQLAPWSAAGQ"
     gene            5253..>5463
                     /locus_tag="PKK95_12440"
     CDS             5253..>5463
                     /locus_tag="PKK95_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19074.1"
                     /translation="MVRDLREELKQTRPFKSLAQEAYLSLQRTAAVLEHELELSLKPS
                     GITATQYNVLRILRGAGSGGLCRGEI"
BASE COUNT          842 a         1911 c         1933 g          777 t
ORIGIN      
        1 gacaaccgct accagctctg ggtgaacggc actcgcgtcg tctttggccc ggcgcgcggc
       61 gacctgaacc actggcgctt cgaaacgatc gatctcgccc cgcacctgca cgccgggcgg
      121 aacgtgctgg cggccgtcgt ctggaactac ggcgagctgg ccccagaggc gcaaaccacc
      181 tggcgcaccg ggtttctcat ccagggcgac acggccgccg aggccgcggt gaacagcggg
      241 accacgtgga aggccgctgc gaacccggcg tactcgccca ttcccttcac ccacgcacag
      301 atgcgcgggt acttcgtcgt cggtcccggc gagcgcgtgg acggcgcgaa gttcccgtgg
      361 gggtgggaga cggagggcta cgacgactcg gcctggagcc gggccgaacc cgccgttccg
      421 gacggccgcg cgcacggctc gcctcgcccg agtcaggatg ccggcaaccg gtggctgctc
      481 gtgccccgct cgatcccgct gatggaggag agcctggagc ggctggccgc ggtccggcag
      541 gcggccgccg tcactgcccc ggaggctttc ccacgcagcg aggcgccgtt cacgatcccg
      601 ccgcgcaccg aggcgcgcct gctcctcgac aacgcgcgcc tcactaccgc ctaccccgtg
      661 ctgcgctaca gcggcggcgc cggcgcccgc atccgcatgg gctacgccga gtcgctctac
      721 ctgccgcggg cgaagggggg tgacaagggg aaccggaacg aggtcgaggg gaaggagttc
      781 gtcggcaact acgacgaggt gatcgccgat ggggggcgcg accgcactta cagcccgctc
      841 tggtggcgga cctaccgcta cctcgagctg accgtcgcca cgcgcgacga ggcgctcgtg
      901 ctccacgacc tgcacggtct ctacaccggc taccccttcg aacgccggca gcgcctcgag
      961 gccgcgcctc cggtggtctc gcagatcctc gacgtcgggt ggcgcaccgc acggctctgc
     1021 gcgcacgaaa gctacatgga ctgcccgtac tacgagcagc tccagtacgc gggcgacacc
     1081 cgcatccagg cgctcgtctc gctctacgcc tcgggcgact cgcgcctggt gaagaacgcg
     1141 atctcgcatt tgaacgactc gcgcaccgcg gagggagcga cgatgagccg ggcgccgacc
     1201 cggcagcagc agtacatccc gccgttctcg ctgtggtgga tcgggatggt gcacgactac
     1261 tacatgtacg tggacgaccc ggcgttcgtc cgcgagatgc tgccgggcgt tcgcgcggtg
     1321 ctgcagttct tcgcggccca ccagcggccg aacggatcgc tggggctgat gccgtggtgg
     1381 aactacgtgg actggacgac cgagtgggac ggcggcgtgc cgccgcgcga cgccaacggc
     1441 gcgagcgccc ctcaagacct tcagctgctg ctcgcgctcg actgggcgag ccggctggag
     1501 gaggcgctcg gctcgcgcgc catcgccgcg gaataccgcg agtcggccgc gcggctgcgc
     1561 ggctccatcc ggacgctcta ctgggacgcg ggccggcggc tgttcgccga cacgcccgac
     1621 cgcaagcagt attcccagca cgcgaacgtc ctcgccgtgc tcgccggcgt cgcttcggga
     1681 gaggaggcgc gcgacctggt gacgcgggtc atcgcggacg agacgttgac ccagtgctcg
     1741 tactacttcc gccactacct gcactcggcg gtgaacctgg tgggggaggg cgatcggtac
     1801 ctcgggctgc tgggtgagtg gaagtcgatg ctggcgcgcg ggctgacgac cttcgccgag
     1861 cggtacgagc ggccgggcaa cccctcccga tccgactgcc acgcctggag ctcgagcccc
     1921 aacttcgaga tcttcaggac ggtgctcggc atcgacagcg ccgcgcccgg gttcgcgcgg
     1981 gtgcaggtgc gcccgttcgt cggaagcctc gaccgggtgt cgggatcgat cccgcatccg
     2041 aaaggggaga tctcggtccg gctcgagcgt gcggggcgcg ggctgcgcgt cgaggtggtg
     2101 ctccccgccg gcgtgccggg cgagttctcg tggggcggga cgcgccgcga actggcgccc
     2161 ggaagcaacg ccttcacggt cgagcgttga cgtctttttc ggagtcgagc cctccctttg
     2221 gaacctcacg ggccgcggac tcgtccctac ctagagccgc ggacagcggg cgtcccggcc
     2281 aatccggagg gagaaccgca tgagtgctcg actccgagcg ggtgcggccg tgtcggccgc
     2341 cgcgctgctg ctgggcttgc aggtggccgc gggcgcgtcg gccggcacca cgcaggaggc
     2401 gccgccgtcg ggcggccgcc tcgacgtgcc cgtcgactac tacaagctgc cgaacggcct
     2461 gaaggtcgtc ctctctcgcg acaccaccac gccgaccgcg gtggtgacca tctactacaa
     2521 catcgggttc cggaacgagc cgaaggaccg cacggggttc gcgcacctct tcgagcacat
     2581 gatgttccag ggctcggcga acctgggcaa gatggagttc atccggctga tcgagtcgaa
     2641 tggcgggctg ctgaacggct cgacccggtt cgacttcacc aactactacc aggtcgtccc
     2701 gtcgcacgtg ctcgagaccg tgctgtgggc cgaggccgac cgcatgcggg gcctcgacat
     2761 caccggggag aacctgaaga accagcagga cgtcgtcaag aacgaggtga aggtcaacgt
     2821 cctcaaccag ccgtacggcg gctttccgtg gatcgacctg ccgatggctg cgaacgagaa
     2881 ctggtataac gcccacaatt tctacggcga cctggagcac ctcgacgccg cgacgctcga
     2941 ggacgtgcaa tcgttcttcc gcacctacta cgcgccgaac aacgccgcgc tcgtggtggc
     3001 gggcgacttc gacccggcgc gggccaagga gtggatcgag cggtacttcg gcgacattcc
     3061 cgccgcgaag cagcctcccg ctcccgacct gcgggagccg cgccaggaga aggagaagcg
     3121 cgccgggcgc accgacccgc tcgccgcacg gcccgcgctc ggcatcgcct accacgtccc
     3181 cgatcggctc acgcctcagt ggtacgcctt cggcctgctc gaccaggtgc tcgcccaggg
     3241 tgcggattcg cttctctacg acgagctggt gaggaagcag gggctgacgg gcgacctgag
     3301 cgctgggatc aactgggggc tcggcggaat gttcgactac agcgggccga tgctgtggat
     3361 ggcccaggtc ttccacgatg cggggacgcc atcggagaag gtcatcgccg ccatcgacac
     3421 cgtcgtcgaa cgcctgcggt cggcgccgct ggacgaggcc acgctggagc gcgcccgcgt
     3481 caagatgcga tcgagcctgt acggcgacct cgagagcttt gccggcttcg ggcgcgccaa
     3541 cgtgctcgcg tccttcgcgc tgttcgacga cgacccgggg aggatcaacc ggctcgaggc
     3601 ggagttcgcg aaggtgacgc ccgcgctcgt ccacgagacg gcgcgcgaat tcctccggcc
     3661 gggcaaccgc accatctaca ccatcgagcc gggcaaggcg gcgccggcga agggaggcgc
     3721 acgatgagtc gcaggaccac tgccctggcc gtcacggccg ccctcctgct gctggcgccg
     3781 ggcgtgcccg gggcgcggga acagcaggcg ccgcccgctt caccccagaa gcaggccccg
     3841 ccgccgactg gcacgccaaa ggacttcgcg ctgcccaagc cctcccggtt cactctgccc
     3901 aacgggatgg cggtcacgat ggtgcccttc ggccaggtcc cgaaggtgac gctgcgcctg
     3961 gtcgtcgcgg ccggcaacct ctacgagggg aaggacgagg tgtggctggc cgacctgacc
     4021 gggcgcatgc tgcgcgaggg gacggcggcg cgtcccgccg ccgaggtggc ccgggagttc
     4081 gccggcatgg gcggcgagct gagcgtcggt gtcggctccg acaacgcgag cctttcggcc
     4141 gaggtcctgg ccgagcgggg cgccgatgcg gcgcggctga tcgccgaggt ggcgcggcag
     4201 ccgctcctgc ccgcgggcga gttggcccgc gtgaaggcgg gcctcacgcg cgatctcgcc
     4261 atccagagga gcacccctca ggcccaggcg caggagcgct tccacgagtc gatctacggc
     4321 gaccacccgt acggccgcct ctttccgacc gaggagatgc tgaacggcta caccatcgag
     4381 cgcgtgcgga cgttccaccg cgcccgctac acgcccgcgg cctcgcggct gtacgtggcc
     4441 ggagtcttcg acgccgccgc gctcgagggc gcgatccgcg acgccttcgg cgcctggaag
     4501 ggaagcgctg gcgcctcgga ggtgcagccg ccgaccgcca aggcggagcg ccggttcgcg
     4561 ctgctcgatc gcccgggcgc gccgcagtcc acgctctacc tcgggcttcg cgtccccgat
     4621 ccgtccagcg ccgactgggt ggcgctgcag gtcaccaact cgctgctggg cgggtcgttc
     4681 ggctcgcgca tcacgaccaa catccgcgag cagaagggct acacctactc gccgttcagc
     4741 gcggtgaatc cgcaccggcg cgatgcgtac tgggccgagg tggccgacgt cacctccgcc
     4801 gacacgggcg ccgccctgaa ggaaatcttc tcggagatcg agcggctccg gcgcgatccg
     4861 cccccggagg gcgagctgcg gggcatcaag aacaacatgg ccggcagctt cgtcgtccga
     4921 aactcgtcgc gccagggcgt gatctcgcag ctggccttcg tggacgagca cgggctgggc
     4981 gacggctacc tggccgggta cgtgaagcgc gtcatggcgg tcacgccgca ggaggtgacc
     5041 cgcgtggccc gggagtatct cgtgccagac cgaatgacgc tcgtcgtcgt cggcgaccgc
     5101 aagacggtcg aggggcagct ggccccgtgg tcagcggcgg gacagtgagg cgaatggtgc
     5161 ccccggccgg ctacccttga cgcgcggtcg agggccggcc ggggcgaccc tcgacacttg
     5221 acaatgagtg tgataacacg tattatggag acgtggtcag ggaccttcga gaggaactga
     5281 agcagacgag gccgttcaag agcctggccc aggaagccta cctgagcctg cagcggacgg
     5341 ccgccgtgct ggagcacgag ctcgagctgt cgctgaagcc ctcggggatt accgccacgc
     5401 agtacaacgt gctgcggatc ctgcgcgggg ccggttccgg cgggttgtgc cgcggcgaga
     5461 tcg
//
LOCUS       DAOPQX010000248         5457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16385_length_5457_cov_3.208812, whole genome shotgun sequence.
ACCESSION   DAOPQX010000248 DAOPQX010000000
VERSION     DAOPQX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16385_length_5457_cov_3.208812"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(424..1320)
                     /locus_tag="PKK95_12445"
     CDS             complement(424..1320)
                     /locus_tag="PKK95_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC19075.1"
                     /translation="MPLRRAAVPAAALLVLAAAAAAFWGAARGAARPADPEPFFFIQI
                     SDPQLGFGHLRGDITQDSANLQFAVAAINRLRPAFVVVTGDLVNTQGNAAQIAEYRRV
                     VAGVDSTVPVHHVAGNHDVGNAPTPESLEAYRKEFGPDRYTIRREGFTGIVINSAVAA
                     APDQVPAEAAAQRKWLEGELRRARAAKSAHLVVFSHHPPYIQDPAEPDGYLNLPREPR
                     DWLLALLARYEVSHLFAGHRHRDLVVQAPGTELVITGSLSRRSTDGPSGLRVVQVSGA
                     GITHRYCHLGELPSRVEPAARW"
     gene            complement(1383..3617)
                     /locus_tag="PKK95_12450"
     CDS             complement(1383..3617)
                     /locus_tag="PKK95_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOC19076.1"
                     /translation="MTNAFARGALCAFLALSWCAAPARAAGGEPLPPPPRPRVGLALG
                     GGSARGLAHIGVLQWLDEHRIPVDLVAGTSAGALVGGGFALGMSPAELRALMRRADWD
                     AMFQADSPFRHKLFRRKQDRRAFPTVLEFGLRHGFMLPGGLNPGQQVMLLLDRMALAY
                     ADLESFDELPTPFRCVATDLRTADSVVLGRGVLATALRATMALPGVFTPVEYGDWLLV
                     DGGTLNNVPGDVARAMGADVVIAVNLGPVRTGARADQSLIRHFGRTIDTMMAAGVRKA
                     LSGVDLVITPELPGLDGMSWRRSDELADLGYQAAARHRDILLTYSVSEREYSAYLAER
                     GARRRTALPVPVSVTVAGVPEAEAASIREQLAGHVGVPVDPDRIEADILSIAGTDRYE
                     YLTYRVTEETGRPCLAVTVRPKSYGPPFLAMGLELNNIDANNFAATVAARTTLYDLAG
                     PGSELRLDVALGTQVLAGGELYQPLGRGPLFGAARASYTRGPANYFEDQRLIAQYSRK
                     RAGAGFDLGLGLVREAELRVGFDVADVHGRLAVGSPVRADVRGWERFGSVRFTHDGQD
                     SPVVPSSGLLLHAELRRYFDAPTPAAPAAGEPVVPTRFWRGEVTGSVFRRVHRADRVF
                     AAFGTGTAFGELPLWGAFSLGGPLRLGAFANDEMRGANYVLGAAGYLRRVARLPDLVG
                     GNVYAGTWLEAGSAFDRRADAAWHGNVSTGAVLETLLGPVFVGGSVGSGWRGRFYVAI
                     GKFF"
     gene            3725..5242
                     /locus_tag="PKK95_12455"
     CDS             3725..5242
                     /locus_tag="PKK95_12455"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOC19077.1"
                     /translation="MNRRDFLKQASLAAIGTSTVIRLGAAEKRVAFGGMQIECSTYGG
                     NLSRLEDFTIRRGKAVSDVPFFSVLKGYPYPFMPTLFATAVPGPPVERKTYDAIKAEF
                     LGRVRDLLPLDGLYLPMHGAMYVEGMLDAEGDWISAVRQVVGEKCLITASYDLHGNIS
                     RRVIDNLDMLSAFRTAPHIDREETHKRACDMLVQSLETGIRPALVWVPIPVLMPGERS
                     STLYDPAKRLWAQLPALNARDGVMDVSLLAGYVWADEPRATAAAVVTGTKIETLREIA
                     RQLGQQYWDARKEFSFGVPTGTMAECIERALKLDTKPVVISDSGDNPTGGGTGDRADA
                     LAELLRMKATSAVVAGIADRPATEACYKAGVGQTLPLKIGGTLDPASSKPIDVTAKVL
                     FLAKTPRLPERQAVVQIEGVTLVLTARRRPFHLPTDFTSLGLEPTSFKIVVVKAGYLV
                     PEIAAFANPNLMALTDGSVNQDIENLESRHRVPTYPFVQDLQWEPVTIVGARSKR"
BASE COUNT          840 a         1910 c         1947 g          760 t
ORIGIN      
        1 tccgtcagtt gctcgatgac ggcctcgcac gtgctccggt ccaagcccca caaccgttgg
       61 gcctgcgaca ccgtcaactt cagccccggc atctcgagga actcgctctt gacccggcgc
      121 aaccgctgct cgcccacgat ccccggcacc atggctcgcc cccttccttc tcagcgttcg
      181 ggacgcggcc gaaccgatcc cctcgctggg caggcggggg actggcgccg gccaccgtga
      241 ccaagtggag cagcagcgat gccaggcgtc cgccgcgtcg gcgtggggcg gaaccgacgt
      301 gttgacagtt ctttagcggg tttgccgttc gaggatcggg ctgggagggt cgcgttgccg
      361 cgacttcgag ccggcagacg gttgcggatg cgcaaccgtg cggtggagaa gcggtgcgga
      421 cggctaccag cgtgccgcgg gctcgacgcg gctcggcagt tcgcccaggt ggcaatagcg
      481 atgcgtgatg cccgcgccgc tcacctgcac gacgcggagg cccgacggtc cgtcggtcga
      541 tcgcctgctc aacgaaccgg tgatgaccag ctcggtgccg ggagcctgga cgaccaggtc
      601 gcggtgccgg tgcccggcga agaggtggct cacctcgtag cgggccagca gcgcgaggag
      661 ccagtcgcgc ggctcgcgcg gcagattgag gtatccatcg ggctcggcgg gatcctggat
      721 gtatggcggg tggtgcgaga acaccaccag gtgggccgac ttggcggccc gcgcccgccg
      781 cagctccccc tcgagccatt tcctctgggc ggctgcttcg gccgggacct ggtccggggc
      841 ggcggcaacg gcggagttga tcacgatgcc cgtgaagccc tcgcgccgga tggtgtacct
      901 gtcgggcccg aactccttcc ggtaggcctc gaggctctcc ggggtcgggg cgttcccgac
      961 gtcgtggttg cccgcgacgt ggtgcacggg caccgtggaa tcgacgcccg cgacgacgcg
     1021 ccggtactcg gcgatctgcg cggcgtttcc ctgcgtgttc acgagatcgc cggtcacgac
     1081 cacgaaggcc ggtcggagcc ggttgatggc ggcgaccgcg aactggagat tcgcgctgtc
     1141 ctgggtgatg tcgccccgga ggtggccgaa tccgagctgg ggatcgctga tctggatgaa
     1201 gaagaagggc tccgggtcgg cggggcgggc ggctccgcgt gccgctcccc agaacgccgc
     1261 ggccgccgcc gcgagaacca acaacgccgc tgcgggaacg gcggctcgtc gtaggggcat
     1321 gttccgtcga ttgtatccca actcggctgt ttcaatgacc gcgcgtggcc gccggaagag
     1381 gctcagaaga acttgccgat cgccacgtag aagcggccgc gccagcccga gccgacgctg
     1441 cccccgacga acacgggccc gagcagcgtt tcgaggactg cgccggtcga cacgttgccg
     1501 tgccaggcgg cgtcggcacg gcggtcgaag gccgacccgg cctccagcca cgtgccggcg
     1561 tagacgtttc cgccaaccag gtcgggcagc cgcgcgaccc gcctcaagta ccccgcggcg
     1621 ccgagcacgt agttcgcccc gcgcatctcg tcgttggcga acgcgcccag cctgagcggg
     1681 cccccgagag agaaggctcc ccagagcggg agctcaccga aggcggtgcc cgtcccgaag
     1741 gcggcgaaga cccggtcggc acggtgcact cgacggaaca ccgaccccgt gacctcgccg
     1801 cgccagaacc gcgtcgggac gactggctcg ccagcggcag gcgcggccgg cgtgggggcg
     1861 tcgaagtagc gtcgaagctc cgcgtggagc agcaggcccg acgagggcac gacggggctg
     1921 tcctgcccgt cgtgggtgaa ccggaccgac ccgaaccgct cccagccgcg cacgtcggct
     1981 cggaccgggg agccgaccgc cagccgcccg tgcacgtcgg ccacgtcgaa gcccacgcgc
     2041 agctccgcct cgcgcactag gccgagtcca aggtcgaacc ccgcgccggc ccgcttccgc
     2101 gagtactggg cgatgagccg ctggtcctcg aagtagttcg cgggccccct ggtgtaggag
     2161 gctcgcgcgg ccccgaacag cggcccccgg ccgagcggtt ggtacagctc gccgccggcg
     2221 agcacctgcg tgccgagcgc cacgtccaac ctgagttcgg atccgggtcc ggcgaggtcg
     2281 tagagcgtgg tgcgcgcagc caccgtcgcc gcgaagttgt tcgcgtcgat gttgttcagc
     2341 tcgagcccca tcgcgaggaa cggcggcccg tacgacttgg ggcgcaccgt caccgcaagg
     2401 cacggccggc cggtctcttc ggtgacgcgg tacgtcaggt actcgtagcg atcggtgccg
     2461 gcgatgctga ggatgtctgc ctcgatccgg tcgggatcga cgggaacgcc cacgtggccg
     2521 gcgagctgct cgcggatgga cgccgcttcg gcctcgggca cgccggcaac cgtgacgctc
     2581 accggcaccg gcagcgccgt ccgccgccgc gcacctcgtt cggcaaggta cgcggagtac
     2641 tcccgctcgc tcaccgagta cgtcaacagg atgtcgcgat ggcgggcggc cgcctggtac
     2701 ccgagatccg ccagctcgtc tgatcgccgc cacgacatcc cgtccagccc gggcagctcg
     2761 ggggtgatga cgaggtccac gccgctcagt gccttgcgca cgccggccgc catcatcgtg
     2821 tcgatcgtgc gaccgaagtg cctgatcagc gactggtccg cccgtgcccc ggtccggacg
     2881 gggcccaggt tcacggcgat gaccacgtcg gcgcccatgg cgcgcgcgac gtcgccgggg
     2941 acgttgttga gcgtgccgcc gtccaccagc agccagtcgc cgtactcgac cggcgtgaag
     3001 acgcccggca gggccatcgt cgcgcgcagg gccgtcgcca gcacgccgcg gccaaggacg
     3061 actgagtcgg ccgtcctgag gtcggtcgcc acgcagcgga acggggtggg cagctcgtcg
     3121 aagctctcga ggtcggcgta ggcgagcgcc atgcggtcga gcagcagcat cacctgctgt
     3181 cccgggttga ggccgccggg gagcatgaag ccgtgcctga ggccgaactc gagcacggtc
     3241 ggaaaggccc gtcgatcctg cttccgccga aacagcttgt gccgaaaggg cgaatccgcc
     3301 tggaacatcg cgtcccagtc ggccctgcgc atcagggcgc gcaactccgc gggcgacatc
     3361 ccgagcgcga aacctccgcc gaccagcgcc ccggcgctcg tgccggcgac gagatcgacg
     3421 gggatgcggt gctcgtcgag ccactgcagc acgccgatgt gcgcgaggcc gcgggcgctt
     3481 cccccgccaa gcgcgaggcc gacgcggggc cgcggggggg gaggaagggg ctcgcccccg
     3541 gcggcgcggg ccggggcggc acaccaggag agggcgagga aggcgcacag ggcgccgcgg
     3601 gcgaacgcgt tcgtcatggc tagcgcgggc cgccgcggcg ccgcgtgacg gcggaaccgg
     3661 cagaccccaa ggggagaccc tgccatcata cgccgggcgt ggtatcgtgt ccgccggagg
     3721 catcatgaac cggcgagact tcctgaagca ggcatcgctc gcggcgatcg gcacgtccac
     3781 ggtcatcagg ctgggggccg cggagaagcg cgtcgcgttc ggcggaatgc agatcgagtg
     3841 cagcacgtac ggcggcaacc tctcgcgtct cgaggacttc accatccggc gcggcaaggc
     3901 ggtctccgac gtgcccttct tcagcgtgct gaagggctac ccgtacccct tcatgcccac
     3961 gctcttcgcg acggccgtgc ccggcccgcc cgtcgagcgc aagacctacg acgccatcaa
     4021 ggccgagttc ctcgggcgcg tccgcgacct gctgccgctg gacgggctgt acttgccgat
     4081 gcacggggcc atgtacgtcg agggcatgct cgatgccgag ggcgactgga tctcggccgt
     4141 ccgccaggtg gtgggcgaga agtgcctcat caccgccagc tacgacctcc acggcaacat
     4201 cagccggcgg gtgatcgaca acctcgacat gctgtcggcc ttccgcacgg cgccgcacat
     4261 cgaccgcgag gagacccaca agcgggcctg cgacatgctg gtgcagtcgc tggagaccgg
     4321 catccggccg gctctggtgt gggttccgat tcccgttctg atgccgggcg agcgcagcag
     4381 cacgctctac gacccggcga agcggctctg ggcgcagctg ccggcgctga acgcgcgcga
     4441 cggcgtgatg gacgtgtcgc tgctcgcggg ctacgtctgg gccgacgagc cgcgcgccac
     4501 ggcggccgcg gtcgtcaccg gcaccaagat cgagacgctg cgcgagatcg cgcggcagct
     4561 cggccagcag tactgggatg cgcgcaagga gttctccttc ggcgtgccga cggggacgat
     4621 ggccgagtgc atcgagcggg cgctgaagct cgacaccaag cccgtcgtga tttccgattc
     4681 cggcgacaac ccgaccggcg gcggcaccgg cgaccgggcc gacgcgctcg ccgagctgct
     4741 gcgcatgaag gcgacgagcg cggtggtggc gggcatcgcc gatcggcccg ccacagaggc
     4801 atgctacaag gccggggtgg ggcagacgct gcctctgaag atcgggggaa cgctcgaccc
     4861 ggcgagcagc aagccgatcg acgtgaccgc gaaggtgctc ttcctggcga agaccccgcg
     4921 gctgcccgag cgccaggcag tggtgcaaat cgagggggtg acgctggtgc tgaccgcgcg
     4981 ccggcggccc ttccaccttc cgaccgactt cacctcgctc ggcctcgagc ccacctcgtt
     5041 caagatcgtc gtcgtgaagg cgggctattt ggtgcccgag attgccgcgt tcgcgaatcc
     5101 gaacctgatg gcgctgaccg acggctcggt gaaccaggac atcgagaacc tggagagccg
     5161 ccaccgcgtg ccgacttatc ccttcgtcca ggacctgcag tgggagccgg tcacgatcgt
     5221 cggcgcgcgt tcgaaacggt gaaccagcgc ccactcctca ggcaacgtca cggtcgacgc
     5281 agagacacgg aggcgcggag acggacgcag agatctctga acatttcaga gatctctgcg
     5341 ccctctacgc ctctgcgttg aacgtccacg tccttgctca aaccaagtga gcgccggggc
     5401 ctctagatcc tgacgacgcg ctcggcgccg gtcagcgtgg ccgcgatctc ccgcatg
//
LOCUS       DAOPQX010000249         5421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16521_length_5421_cov_3.832464, whole genome shotgun sequence.
ACCESSION   DAOPQX010000249 DAOPQX010000000
VERSION     DAOPQX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16521_length_5421_cov_3.832464"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PKK95_12460"
     CDS             <1..207
                     /locus_tag="PKK95_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19078.1"
                     /translation="LALYGLADDTFEQFVPCVERVTAEEVIAAAGRLDLRRMVVAVVG
                     DREQVLPGLEALGLGEPQVLEPPD"
     gene            complement(243..407)
                     /locus_tag="PKK95_12465"
     CDS             complement(243..407)
                     /locus_tag="PKK95_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19079.1"
                     /translation="MIEQPAPSPTPPGPKPPDIVDKPPPDPRPVPPPDVPPLPRPDIP
                     GEPQQPGLAP"
     gene            462..1505
                     /locus_tag="PKK95_12470"
     CDS             462..1505
                     /locus_tag="PKK95_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC19080.1"
                     /translation="MLARFFSRWERRLYERTTGRRVRPFEWGLDWLGLDRPGDRRPAA
                     EILGEWALAAQACSTDFFAAPPTDDYRWQASEGPEAGTVTFPSAVRTPHPCNDLVSGR
                     LFRVERRGDGRQDRRRAVVVLPQWNADWNGHVGLCRLLARFGVNALRLSLPYHDRRMP
                     PELSRADYIVSSNLGQTLHVCRQAVVDARRAVAWLEREGFERIGILGTSLGSCLAMLT
                     TAHEPRIRAQALNHVSLYFADVIWEGLSTEHVRRGLDGYVTLDRLREWWMPISPAAYV
                     RRVKGTPTLLVSARYDTTFPLHLSMALVEACRREGVDFELTLLPCGHYTTGLAPFKYL
                     DGWALVKFLRKRL"
     gene            complement(1518..1706)
                     /locus_tag="PKK95_12475"
     CDS             complement(1518..1706)
                     /locus_tag="PKK95_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19081.1"
                     /translation="MSLSLFGTVAVVAALLAVATIWLMLTDPVTVANSVNEGDISPLV
                     RQLASVMYEALRGLLKYL"
     gene            complement(1875..3227)
                     /locus_tag="PKK95_12480"
     CDS             complement(1875..3227)
                     /locus_tag="PKK95_12480"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOC19082.1"
                     /translation="MPFQPSETILSAAARIAAGAVSSVALTEAALSRIAERNPALNAF
                     ITVTADLALEQAREADREIAARGPRGPLHGIPISLKDLIDLAGVATTAASRVLRDNLA
                     HRDAVVVSRLREAGAVFLGKTNLHEFAFGTTSEDSAYGPVRNPHDLARSPGGSSGGSA
                     AAVADGLGYGSIGTDTGGSIRIPAAACGCVGLKPTLGELSCEGVVPLSWSLDHVGPIT
                     RSVGDAWLLYDAMRGAPRLLPAAKHAIATRPPRLGVLGGYFTELLEPAVRTSFDAAVR
                     RLADAGAGIERREVAEAARTPATYLRIQLPEATAYHAATLEREPGWYTPAVRSRLEMG
                     RDVSAVDYVNARRDALVLRDAVDAALQGCEALALPTLPILAPPLGSGGMQLEGESVPV
                     RVLMLRLTQLFDVTGHPAISLPCGIAGPGLPVGLQLVGRRDATPALLQVASWCERALE
                     "
     gene            3290..4717
                     /gene="pyk"
                     /locus_tag="PKK95_12485"
     CDS             3290..4717
                     /gene="pyk"
                     /locus_tag="PKK95_12485"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOC19083.1"
                     /translation="MRHTKIVATIGPASDTDAALDALIAAGVDVVRLNFSHGTREGHR
                     VVFGRVRAAAERAGRHVAVLQDLSGPKIRIGELEGGQPLQLRAGDELTIATGDRAGGP
                     GVVYTSFTGLAPSVRPGDRLLLDDGRIELQVMAGDGESIRAVTVHGGLLGPHKGINAP
                     GVALAASALTAKDAEDLRLGLALGVDLVALSFVQTRDDLERTRAVMREAGRSGVPLVA
                     KIERPQAAERLPELLEACDGIMIARGDLGLEVPLERVPRIQREATLAARARGIPVILA
                     TEVLDSMRVQPRPTRAEVSDAANAVQDGVDAIMLSGETAIGVDPPHVVRTLNAIIEDA
                     EKGPPPAAVPEGVEDREGHAGALVAAALTLACRGHAEAIVAVTRSGRTARLLSALRPE
                     ARVHAIAPTPEVARRLALLWGVRPQVMDLGGGLLPLAALASRLRAEGALPPGATVVLV
                     SVNPDLGRGDANFLQLLQVPSTLTP"
     gene            4714..>5421
                     /locus_tag="PKK95_12490"
     CDS             4714..>5421
                     /locus_tag="PKK95_12490"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOC19084.1"
                     /translation="MNDSMTAVTPAILAGAAVAAFLLASLVVGLGRRFINRAFARLDV
                     SADDGRAALRARARQLVTALAVVAFGIAGVATLAFVLSLLRVEVPDWTPRQVLAWTLS
                     HGLRALIVLVLAYVALRASSVAIERFKVRLSGDPAVAGSPERLRRAATLSSIVSSLVT
                     ATIIFLAGLMVLRELSIDVVPLLTGAGIAGLAIGFGAQNLVRDVISGFFMILENQVGV
                     GDVARIQNVTGVVEEIKL"
BASE COUNT          726 a         1889 c         2004 g          802 t
ORIGIN      
        1 ctggcgctat acggcctcgc cgacgacacc ttcgagcagt tcgttccctg cgtggagcgc
       61 gtcaccgcgg aagaggtcat cgccgcggca ggccgcctcg atctccggcg gatggtcgtt
      121 gccgtcgtcg gcgaccgcga gcaggtgctc cccggcctcg aggccctcgg cctcggcgaa
      181 ccccaggtgc tcgagccgcc agactgagcg cggcaggacg tgcgagcctt ccccgtccca
      241 aatcagggcg ccaggcccgg ctgctgcggt tcgcccggga tgtcggggcg cggcagaggc
      301 gggacgtcgg gcggcggcac cggccgtgga tcgggcggcg gcttgtccac gatgtcaggc
      361 ggcttcggtc ccggtggcgt cgggctgggc gctggttgct cgatcatgat cccccgcggc
      421 tgcaacgcgg gtgccggttc ccgttggtag aatcctcgac gatgctcgca cggttcttct
      481 cccgctggga acgacggctg tacgagcgga cgacggggcg ccgggtgagg ccgttcgagt
      541 ggggcctcga ctggctcggc ctcgaccgtc ccggcgatcg ccgcccggcc gccgaaatcc
      601 tcggggaatg ggcgctggcg gctcaggcct gctctaccga cttcttcgca gcgccgccaa
      661 ccgacgacta ccgatggcag gcctcggagg gtcccgaggc cggtaccgtc acgttcccga
      721 gcgccgttcg caccccgcac ccctgcaacg atctcgtcag tggccggctg ttcagggtgg
      781 agcggcgcgg cgacggccgg caggatcgcc gccgcgccgt tgtggtgctg ccgcagtgga
      841 acgccgactg gaacggccac gtcggcctct gccggctgct ggcccggttt ggcgtcaatg
      901 ccctgcggct cagccttccc taccacgacc ggcgcatgcc gcccgagctc agccgcgccg
      961 actacatcgt cagctccaac cttggccaga cactgcacgt ctgccggcag gcggtcgtgg
     1021 acgcccggcg ggcggtcgcc tggctcgaac gcgaggggtt cgagcggatc gggatcctcg
     1081 gcaccagcct tggctcgtgc ctggccatgc tgacgacggc gcacgagccg cggatccgcg
     1141 cgcaggcgct gaaccacgtg tcgctgtact tcgccgacgt gatctgggaa gggctttcca
     1201 ccgagcacgt tcgaaggggg ctggacgggt acgtcacgct cgatcggctc cgtgagtggt
     1261 ggatgcccat ctcgcccgcc gcgtacgtgc gccgcgtgaa gggcacgccg acgctgctgg
     1321 tttcggcgcg ctacgacacg accttcccgc tccacttgtc gatggcgctc gtcgaagcgt
     1381 gccgacgcga aggcgtggac ttcgagctga cgctgctgcc ctgtgggcac tacaccacgg
     1441 gcctcgctcc gttcaagtac ctggatggat gggcgttggt gaagttcctg aggaagcggc
     1501 tctagatgta ggaggggcta cagatatttc aacaggccgc ggagggcctc gtacatgacc
     1561 gaggccagct gccgcaccag cggggagatg tcgccctcgt tcaccgagtt ggcgaccgtg
     1621 acggggtcgg tgagcatcag ccagatggtg gcgaccgcca gcagggccgc cacgacggcg
     1681 accgtgccga acaagctgag gctcatgcgg gccaggccca tctttcttga cctcgcggct
     1741 gtagctctag tctagcacca accttcgcca ccaacggggc gccgaaggac gacgcgatac
     1801 acatgtaaga cgcgccggag aagggagtgg ttcgcaagcg tcgtacgcgc tccgccggcg
     1861 tctcattcgg cgcctcattc gagcgcccgc tcgcaccacg aggccacctg gagcagcgcg
     1921 ggcgtcgcat cccgccgtcc caccagctgc agcccgaccg gaagccctgg ccccgcgatc
     1981 ccgcacggga gcgagatggc cgggtggccc gtcacgtcga agagctgcgt caggcgcagc
     2041 atcagcactc gaacggggac cgattccccc tcgagctgca tgccgccgct accgagggga
     2101 ggcgccagga taggcagcgt cggaagcgcc agcgcctcgc atccctggag cgccgcgtcc
     2161 accgcgtcgc gaagcaccag ggcatcgcgc cgtgcgttca cgtagtccac cgcgctgacg
     2221 tcgcggccca tttcgagccg gcttctgacg gcgggcgtat accagcccgg ctcgcgctcg
     2281 agcgtcgcgg cgtggtacgc ggtcgcctcc ggcagctgga tacgcaagta ggtggctggc
     2341 gtgcgagcgg cctccgccac ctcgcgccgt tcgattccag cgcccgcatc ggcgagccgc
     2401 cggacggctg cgtcgaacga cgtccggaca gccggctcga gcagttcggt gaagtagccg
     2461 ccgaggacgc caagccgcgg aggccgcgtc gcgatcgcgt gtttcgccgc cggaaggagc
     2521 cggggcgctc cccgcatggc gtcgtagagc agccacgcgt cgcccaccga tcgggtaatc
     2581 gggccaacgt ggtccagtga ccaactgagt ggcacgaccc cctcgcacga cagttcgccg
     2641 agggtgggct tcaggccgac gcacccgcac gcggccgccg ggatccgtat cgacccgccc
     2701 gtgtcggtcc caatcgagcc gtaccccagg ccgtctgcca cggccgccgc cgatccgccg
     2761 ctcgatccgc ctggcgacct cgcaaggtcg tgggggttcc ggacgggccc gtaggccgag
     2821 tcctcgctgg tggtcccgaa ggcgaactcg tgcaggttgg tcttcccgag gaacacggcg
     2881 cccgcttcgc ggagccgcga cacgacgacc gcgtcccggt gggccaggtt gtccctcagc
     2941 acgcgcgagg cggcggtggt cgcgacgccg gcgaggtcga tcaggtcctt cagggagatt
     3001 gggatgccgt ggagcggtcc cctcggtccc cgggcggcga tctcgcgatc ggcctcgcgc
     3061 gcctgttcga gggcgagatc ggccgtcacg gtgatgaacg cgttgagggc cgggttgcgc
     3121 tcggcgatcc gggagagcgc cgcctcggtc aacgccaccg acgagacggc ccccgccgca
     3181 atcctggcgg cggccgagag gatggtttcg ctgggttgga acggcatggc acttctggaa
     3241 gggcgcccgg gcttggtgcg cgacaggggg gattgtaagc tggttgcctg tgcggcacac
     3301 gaagatcgtc gccaccatcg gtcccgcctc cgacaccgac gcggcgctcg acgccctgat
     3361 cgccgccggc gtggacgtcg tcaggctcaa cttctcgcac ggcacccgcg aggggcaccg
     3421 ggtcgtgttc ggccgcgtgc gagcggccgc cgagcgggcc ggccgccacg tcgcggtgct
     3481 gcaagacctg tcggggccga agatccgcat cggggaactg gagggtgggc agcctctcca
     3541 actccgcgcg ggcgacgagc tgaccatcgc gaccggcgac cgcgcgggcg gccccggcgt
     3601 cgtctacacc tccttcacgg ggctggcacc gagcgtccgt cccggcgatc gccttctcct
     3661 ggacgacggg cggatcgagc tgcaggtgat ggccggcgac ggcgagtcga tccgggcggt
     3721 gaccgtgcac ggcgggctcc tcgggccgca caaggggatc aacgcgcccg gcgtcgccct
     3781 ggccgcctcg gcacttaccg cgaaggatgc cgaggacctg cgcctggggc tcgcgctcgg
     3841 cgtcgacctg gtggcgttga gcttcgtgca gacgcgggac gacctcgagc ggaccagggc
     3901 ggtgatgcgc gaggcgggcc ggtccggggt gccgctggtg gcgaagatcg agcggccgca
     3961 ggcggccgaa cgccttcccg agctgctcga ggcgtgcgac gggatcatga tcgcgcgcgg
     4021 cgacctcggg ctcgaggtgc cgctcgagcg ggtgccgcgc atccagcgcg aggcgacgct
     4081 ggcggcacgg gcgcgcggca tcccggtgat cctcgcgacc gaggtgctcg actcgatgcg
     4141 cgtgcagccg cgcccgaccc gtgccgaggt gagcgacgcg gccaacgcgg tgcaggacgg
     4201 cgtggacgcg atcatgctgt cgggggagac ggcgatcggg gtggacccgc cgcacgtggt
     4261 gcggacgctg aacgcgatca tcgaggacgc cgagaaggga ccgccgccgg ccgcggtgcc
     4321 cgagggggtg gaggatcgcg aagggcacgc cggcgcgctg gtcgccgcgg cgctgacgct
     4381 cgcctgccgg gggcacgccg aggcgatcgt ggcggtgacg cgcagcgggc ggacggcacg
     4441 gctgctctcg gcgctgcgcc ccgaggcgcg ggtccacgcg atcgcgccca ccccggaggt
     4501 cgcgcgccgc ctcgcgctgc tgtggggcgt gcggccgcag gtgatggacc tgggcggcgg
     4561 gctgctcccg ctggcggcgc tggcctcgcg actgcgggcg gagggggccc ttccgcccgg
     4621 cgcgacggtg gtgctggtga gcgtgaaccc cgatctcggg cgtggcgacg cgaacttcct
     4681 gcagctgcta caggtcccga gtacactgac gccgtgaacg actcgatgac cgccgtcaca
     4741 ccggccattc tcgcgggcgc cgcggtcgcc gccttcctgc tggcgtcgct ggtcgtcggc
     4801 ctggggcggc gcttcatcaa ccgggcgttc gcccgcctcg acgtgtcagc agacgacggg
     4861 cgggccgcgc tgcgggcgcg ggcgcgccag ctggtgaccg cgctggctgt cgtggcgttc
     4921 gggatcgcgg gggtagcgac gctcgcgttc gtgctgtcgc tcctgcgcgt cgaggtgccc
     4981 gactggacgc cgcgccaggt gctcgcctgg actctctcgc acggcctgcg cgcgctcatc
     5041 gtcctggtgc tcgcctacgt ggcgctgcgt gcctcgagcg tcgcgatcga acgcttcaag
     5101 gtgcggctct ccggcgatcc ggccgtcgcc ggcagccccg agcggcttcg ccgcgccgcc
     5161 accctcagca gcatcgtctc gagcctggtc accgcgacaa tcatcttcct cgcggggctg
     5221 atggtgctcc gcgaactgtc gatcgacgtc gtacccctgc tgaccggcgc ggggatcgcg
     5281 gggctggcga tcgggttcgg cgcgcagaac ctggtgcgcg acgtgatctc ggggttcttc
     5341 atgatccttg agaatcaggt gggcgtgggt gacgtggccc ggatccagaa cgtgaccggc
     5401 gtggtggagg agatcaagct g
//
LOCUS       DAOPQX010000250         5330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16902_length_5330_cov_4.682844, whole genome shotgun sequence.
ACCESSION   DAOPQX010000250 DAOPQX010000000
VERSION     DAOPQX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16902_length_5330_cov_4.682844"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="PKK95_12495"
     CDS             <1..1730
                     /locus_tag="PKK95_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19085.1"
                     /translation="FVYVALVSMVLVAVGLAAQAGRSGQGVVRVEITYGKALKDGPLD
                     GRLLVMLSTDPGAEPRFQINDSPGTQVIAGIDVDGWRPGQPAVVDGRAVAYPLQSLDE
                     IPPGTYTVQALLQVYETFHRADGKVVKLPMDHGEGRQWNRAPGNLYSTPQTIRVTPGG
                     VVRISLDQVIPPVPDPPETKYIKHVRIQSEKLTKFWGRPMFLGAHVLLPAGFDDHPGA
                     RYPLVIDHGHFPYTFEGFREEPPDPDLKPVYSERFHWEGYNRTEQEQAHQLYKDWTSP
                     GFPRFLLIQIQHANPYYDDSYAVNSANLGPYGDAINEELIPHIEQKFRGLGQGWARFM
                     YGGSTGGWEALGVQILYPDFYNGCFAACPDPIDFRQYTVVNIYEDANAFFVEGPWRRI
                     PRPGSRNYLGHVSTTIEAMNRLELVLGEKGRSGGQWDIWQAVYSPAGADGYPKPIFDK
                     RTGKIDREVAAYWKEHYDLGHILRRDWATLGPKLRGKIHIYVGEADNYFLNNAVYLVE
                     EFLKSTTDPHYDGEVDYGARAEHCWNGDHTRPNAIARLRYPQMFAPKILERILKTAPQ
                     GADVTSWRY"
     gene            complement(1754..2692)
                     /locus_tag="PKK95_12500"
     CDS             complement(1754..2692)
                     /locus_tag="PKK95_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19086.1"
                     /translation="MFFPAPCALSFQAGYRCRHSGACCTAGWPIPVDEGRAALIRAGS
                     AQRLVPAGNFLPKDSSGACGFYDRDARLCRIHARLGLDALPISCAHFPRRLLIEDDRI
                     AISLSHFCPTVAGMLFDPGATGCPAGQPGIVEAPRLLEGIEPEGLDARGAWPPLLRPG
                     VLAGVAAYRLWERAVVEMLAAAEGGADAALARAAAMTREIAGWTPGAGPLESWVVARA
                     RASSRSPVVDPPAASDWGGHDPAVGRYLAAKAFANWVAYLGDGLQAVLGSVERALAVL
                     REEAAAACARASRPLDRPLLTEAFRRADLRLMHLDR"
     gene            2855..3997
                     /locus_tag="PKK95_12505"
     CDS             2855..3997
                     /locus_tag="PKK95_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOC19087.1"
                     /translation="MPSHLRYAALLVVLPLLQSCSTPGAAGDAARGPGGSGRGGGDAV
                     PVLTARVEQKSMPVTLPAVGTVEAVSSVDVRAQVTGQLSAIHFTEGQDVKKGQPLFSL
                     DPRPFQAALKQAEAVLARDAATLRNTEAQEERIASLFQRGLIPRDQFESQQASKAAQE
                     AVVAADRAAVENARLNLEYTEIVAPISGRTGALGVHVGDLVRANDAGALVVINQLTPA
                     YVSFSVPGRHLADIRRFQASRPLAVTASPNGDASGPVERGVVSFIDNAVDPTTGTIRL
                     KATFQNAVRQLWPGAFVRVTLDLTTDPAALVVPAPAVQVSQNGQYVYVVKPDQTVEMR
                     TIELARQQGDEAVIASGLAAGEVVVTDGQLRLTPGAKVSERRGGER"
     gene            4013..>5330
                     /locus_tag="PKK95_12510"
     CDS             4013..>5330
                     /locus_tag="PKK95_12510"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC19088.1"
                     /translation="MNISEPFIRRPVATTLLVLSILIFGAMGYGLLPVNDLPTVDFPT
                     LQVNANLPGASPETMAASVATPLEKQFSTIAGISSISSTNTLGSTSITLQFDLTRSID
                     AAAQDVQSMIARAQRQLPPGMPTPPSYRKVNPADSPVLFLTLSSATLPLSQLDRYAQN
                     LLAQRLSMVSGVAQVMIFGSQKFAVRVDVDPMQLASRQIGIDQVASAIAAANSNRPTG
                     TLYGPDRNFVVQASGQLLEAAGYEPIVVAWRNGSPVRLNEVAHVYAGVEDERNAGWYN
                     GTRSVYLAVQRQPGTNTVQVVDRIKALLPRLQEQLPASVELNIRSDRAQPIRESVHDV
                     KFTLVLTVCLVVLVIFLFLRNTSATIIPSLALPFSIVGTFAVMYLLDYSLDNLSLMAL
                     TLAVGFVVDDAIVMLENVVRHMEMGKPRLQAALDGSKEVAFTIVSMT"
BASE COUNT          868 a         1855 c         1847 g          760 t
ORIGIN      
        1 cgttcgtcta cgtcgcgctc gtctcgatgg tgttggtcgc tgtggggttg gcagcccagg
       61 ccgggcgttc cggccagggc gttgtgcgcg tcgagatcac gtacggcaag gcgttgaagg
      121 atggtccgct cgatggccgg ctgctcgtca tgctctcgac cgaccccggt gccgagcccc
      181 gctttcagat caacgacagc cccggcaccc aggtgatcgc gggcatcgac gtggacggct
      241 ggcggcctgg ccagccggcg gtggtggacg gccgcgcggt ggcttacccg ctgcaaagcc
      301 tggacgagat tccgcccggc acgtacaccg tgcaggcgct gctgcaggtg tacgagacgt
      361 tccatcgcgc cgacggcaag gtggtgaagc tgccgatgga ccacggcgag ggacggcagt
      421 ggaaccgggc gccggggaac ctgtacagca ctccgcagac gatccgtgtg acgcctggcg
      481 gcgtggtgcg gatctcgctc gaccaggtca tcccgccagt ccccgacccg cccgagacca
      541 agtacatcaa gcacgtgcgg atccagagcg agaagctcac caagttctgg ggccggccca
      601 tgttcctggg ggcgcacgtg ctgctgcccg ctggcttcga cgaccacccc ggcgcccgct
      661 atccgctggt catcgaccac ggccactttc cgtacacctt tgagggcttc cgggaggagc
      721 cgcccgatcc cgatctgaag ccggtgtact ccgagcgctt ccactgggag gggtacaacc
      781 gcaccgagca ggagcaggcc caccagctct acaaggactg gacctcgccg ggcttcccgc
      841 gcttcctgct gatccagatc cagcacgcca acccgtacta cgacgactcg tacgcggtga
      901 attccgcgaa cctcgggccg tacggcgacg cgatcaacga ggagctgatc ccgcacatcg
      961 agcagaagtt ccgcggcctc ggccagggtt gggcgcggtt catgtacggc gggtccacgg
     1021 gcgggtggga agccctcggc gtgcagatcc tgtaccctga cttctacaac ggctgctttg
     1081 ccgcctgccc cgaccccatc gacttccgcc agtacaccgt cgtcaacatc tacgaggacg
     1141 cgaacgcgtt cttcgtcgag ggcccatggc ggcggatccc gcgtcccggc agccgcaact
     1201 acctcgggca cgtcagtacg accatcgagg cgatgaaccg cctcgagctg gtgctgggcg
     1261 agaaggggcg ctcgggcggg cagtgggaca tctggcaggc cgtctactcg ccggccggcg
     1321 ccgacggcta cccgaagccg atcttcgaca agcggaccgg caagatcgac cgggaggtcg
     1381 cggcgtattg gaaggagcac tacgacctcg ggcacatcct gcgccgcgac tgggcgacgc
     1441 tcggcccgaa gctgcggggg aagatccaca tctacgtggg cgaggccgac aactacttcc
     1501 tcaacaacgc ggtttatctc gtcgaggagt tcctgaagag caccacggac ccgcactacg
     1561 acggcgaggt ggactacggg gcgagggccg agcactgctg gaacggcgac cacacgcggc
     1621 cgaacgcgat cgcgcgcctt cgctacccgc agatgttcgc gccgaagatc ctggagcgga
     1681 ttctgaagac ggcgccccag ggcgccgatg tgacgagttg gaggtactga cagacttcgt
     1741 ggacgggccg gcgtcagcgg tccaagtgca tcagccgaag gtccgcgcgc cggaacgctt
     1801 cggtcagcag cgggcggtcg aggggacggc tcgcgcgggc gcacgccgcg gccgcttcct
     1861 cccgcagcac cgcgagcgcc cgctccaccg agcccagcac ggcctgcagc ccgtccccca
     1921 ggtaggcgac ccagttggcg aacgcctttg cggccaggta gcgcccgacc gccgggtcgt
     1981 ggccgcccca gtcggacgcc gcgggaggat ccacgacggg cgatcggctt gacgcacggg
     2041 cgcgggccac gacccacgat tcgagcggcc ccgctccggg cgtccatcca gcgatctcgc
     2101 gcgtcatcgc cgccgcgcgt gcaagggccg catcggcgcc accctccgcg gccgcgagca
     2161 tctcgaccac agcgcgctcc cacaggcggt acgccgccac ccctgccaga acacccggcc
     2221 tgagcaaggg gggccaggcc cctcgtgcat cgagcccttc aggctcgatg ccctcgagca
     2281 ggcgcggcgc ctcgacgatt ccaggctgac cggccgggca gccggtcgca ccggggtcga
     2341 agagcatgcc ggcgaccgtg gggcagaagt gcgagagaga gatcgcgatg cggtcgtctt
     2401 cgatcaagag ccggcgtgga aagtgggcgc acgagatcgg aagggcatcc agccccaggc
     2461 gtgcgtggat gcggcacagc cgtgcgtcgc ggtcgtagaa gccgcacgcg cccgacgagt
     2521 ccttcgggag gaaatttccc gcggggacga gccgttgggc cgacccggcc cggatgaggg
     2581 ccgcccgtcc ctcgtccacg gggatcggcc acccggccgt gcagcacgcc cccgagtggc
     2641 ggcaccggta gccggcctgg aacgagaggg cgcagggggc ggggaagaac atgaattgca
     2701 gcgaatgcag aatgcagagc gaaaagggac aaggtcaacg ggaacggcca ccggcaaaaa
     2761 gaagggaacg ccgtaagcgt ggccggcctg gcgtcgtaga tgatactgtc cccgggttcg
     2821 ggattggtgt gccttgtcgc tgctgagctc ttacatgcca tcacacctgc gttacgccgc
     2881 gttgctggtc gtcctgcccc tcttgcagtc ctgctcgacc ccgggcgccg ccggggacgc
     2941 cgcccggggg ccgggcggca gcggccgcgg aggtggagac gcggttcccg tcctcaccgc
     3001 gcgggtcgag cagaagtcga tgcccgtgac cctgccggcc gtggggacgg tcgaggccgt
     3061 gtcgagcgtg gatgttcgcg cccaggtcac cgggcaactg agcgcgatcc acttcaccga
     3121 ggggcaggac gtcaagaagg gccagccgct gttcagcctc gatccccggc cgttccaggc
     3181 ggcgctgaag caggccgagg ccgttctcgc gcgggacgcg gcgaccttgc ggaacaccga
     3241 ggcgcaggag gagcggattg ccagcctgtt ccagcgcggc ctgatccccc gcgatcagtt
     3301 cgagagccag caggccagca aggccgcgca ggaagcagtc gtggccgccg acagggcggc
     3361 ggtcgagaac gcccggctga acctggagta cacggagatc gttgcgccca tctcggggcg
     3421 caccggcgcg ctcggcgtcc acgtcggcga cctggtccgc gctaacgacg ccggagcgct
     3481 cgtggtgatc aaccagttga ccccggccta cgtcagcttc tcggtgccgg gccggcacct
     3541 ggccgacatc cgccgcttcc aggcgagcag gccgctcgcg gtgacggcga gcccgaacgg
     3601 cgacgcttcg ggccccgtcg agcgcggcgt cgtcagcttc atcgacaacg cggtcgatcc
     3661 caccaccggg acgatccggc tcaaggccac cttccagaac gccgtgcggc agctgtggcc
     3721 gggggccttc gtccgcgtga cgctggacct cacgaccgac cccgccgcgc tcgtcgtgcc
     3781 ggcgcccgcc gtgcaggtct cccagaacgg ccagtacgtc tacgtcgtca agcccgacca
     3841 gacggtggag atgcgcacca tcgagctggc acgccagcag ggcgacgagg cggtgatcgc
     3901 gtcgggcctg gccgccggcg aggtggtggt caccgacggg cagctcaggc tcacgcccgg
     3961 ggcgaaggtg tcggagcggc gcggcggcga gcggtaggga cgggagccag ccatgaacat
     4021 ctccgagcca ttcatccgcc ggcccgtcgc caccacgctg ctggtgctct cgatcctgat
     4081 cttcggggcg atgggctacg gcttgctgcc ggtcaacgac ctgccgacgg tcgattttcc
     4141 aacgcttcag gtcaacgcga acctccccgg ggccagcccc gagacgatgg ccgcgtcggt
     4201 ggcgacgccg ctcgagaagc agttctcgac catcgcgggg atcagctcga tcagctccac
     4261 caacacgctg ggcagcacca gcatcacgct gcagttcgac ctgacccgat cgatcgacgc
     4321 ggcggcgcag gacgtgcagt cgatgatcgc ccgggcgcag cgccagctcc cgcccgggat
     4381 gccgacgccg ccgtcgtacc gcaaggtgaa ccccgccgac tcgccggtgc tgttcctcac
     4441 gctcagctcg gcgacgctgc cgctgtcgca gctcgaccgc tacgcgcaga acctgctcgc
     4501 gcagcgcctg tcgatggtga gcggggtggc gcaggtgatg atcttcgggt cgcagaagtt
     4561 cgcggtgcgc gtggacgtcg acccgatgca gctcgcctcc cggcagatcg gcatcgacca
     4621 ggtggcctcg gcgatcgccg ccgcgaactc gaaccgcccc accggcacgc tctacggccc
     4681 cgaccgcaac ttcgtcgtcc aggcgtcggg ccagctgctc gaggccgccg gctacgagcc
     4741 gatcgtcgtg gcgtggcgca acgggagccc ggtccggctg aacgaggtgg cgcacgtcta
     4801 cgccggcgtc gaggacgagc ggaacgcggg atggtacaac ggcacgcgca gcgtctacct
     4861 ggccgtgcag cggcagccgg gcaccaacac ggtgcaggtg gtggaccgca tcaaggcgct
     4921 gctgccccgg ctgcaggagc agctgcccgc ctcggtggag ctgaacatcc gcagcgaccg
     4981 ggcgcagccg atccgcgagt cggtgcacga cgtgaagttc acgctcgtgc tcaccgtctg
     5041 cctggtcgtg ctggtcatct tcctgttcct gcggaacacg tcggcgacga tcatcccgag
     5101 cctcgcgctg ccgttctcga tcgtcggcac cttcgccgtc atgtacctgc tcgactacag
     5161 cctcgacaac ctgtcgctga tggcgctcac cctcgcggtc ggcttcgtcg tggacgacgc
     5221 catcgtgatg ctggagaacg tcgtccggca catggagatg gggaaaccca ggctgcaggc
     5281 ggcgctcgac gggtcgaagg aggttgcctt caccatcgtc tcgatgacgc
//
LOCUS       DAOPQX010000251         5317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16941_length_5317_cov_4.415241, whole genome shotgun sequence.
ACCESSION   DAOPQX010000251 DAOPQX010000000
VERSION     DAOPQX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16941_length_5317_cov_4.415241"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKK95_12515"
     CDS             <1..473
                     /locus_tag="PKK95_12515"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOC19089.1"
                     /translation="READVVLSPADGRVVIAGDPQPGLPPPGAWRQVSIFLSPLDVHV
                     NRMPVAGRVSRIEYRPGRYRAAYDEAAGAENERNEIWIDRDGGPVVFRQITGVLVRRV
                     VCRARVGDVVAAGDRFGVMKFGSRIDLFLPASARLLVSPGDRVRAGETVVARLA"
     gene            480..1316
                     /gene="pssA"
                     /locus_tag="PKK95_12520"
     CDS             480..1316
                     /gene="pssA"
                     /locus_tag="PKK95_12520"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOC19090.1"
                     /translation="MQLELARDHRPRPPHPLKRRTAEQRRRFRRGVYLLPSVFTLGNM
                     FCGYICIVYSIRGDFETAAPFIAFSVVLDMLDGRIARLTGSTSAFGLEFDSLADVVSF
                     GVAPAILTVSWGLTPLGRLGWAAGFIFVTAGAIRLARFNIQASTDKRHFVGMPIPAAA
                     AVPASTVYVYPAGLQDVGAALPALALMIVPAVLMVSTIRFRSFKSIDLRWRRSSRVLL
                     LLALFLMAIATHPRWTLLALAYTYLLSAFVAMAAARLRRRAAPAGAPISPPPPPEPPP
                     AG"
     gene            complement(1276..5226)
                     /locus_tag="PKK95_12525"
     CDS             complement(1276..5226)
                     /locus_tag="PKK95_12525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC19091.1"
                     /translation="MRLASRRQSLIAILVAGLAATLLVVIGGWAVERVRLGPNDRAAA
                     RRVEGSVRAQLDRVSHALRGAASDLAEAARPSLAHPADERDLRPLFGRADELTSDPDA
                     RTIAVTIYGTDGSPLAWAGRPSQVPVERLNGGPSLFVSRGPVGVRLVATAPVTGPGSG
                     GPLLGTIAAEALLSTVSGMPGHAGSTPTMPTPLAPVALRPASEIARDQPSGRHAFLLH
                     DAGGRPLVEASVAYEDLAEARARHRRGVVDLAWLAAALTVLLLAGPLLDGRQRARTRG
                     EYCVPTVAVAVLLLVARGLLWLIRGPGGSRLFSPAIYKSAALGAWFRHPADFLATGLL
                     AIALVALIAGPAERWRIGRRSRRTSLPRGASAARFVAAQAVAGLALVAAVALFERFLG
                     DTFASSGTDILYFSPHPWNAGRVALGAGLVLFHAAFAWAAVLGLRSALAFWLVGAEPR
                     AVALLALAWGAPVVLGWAWWGGTSGLPRSGALLASAGLAAAAFHAPRGLTRLRHASQG
                     YRLAAMFVALLLPSLLFYPSLVFFEDVAKRRVIEGRYAPEVVHQLENLQRRVQTAQQE
                     IDRRDDVLRSVVSVEAPPPGSPIPPDSAFLVWQGTALERHRVSSAVELYNGGQWLVSR
                     FALNLPEDASRQRWREEGCEWRTFGESSPFGAQERQLLHAGRNICGEDGILGTIVIHG
                     ILDDSTLSFLSSQDPYFELLRGGALLPREAAPGREIEYVVYGWSRSAIYLSGARAWPL
                     DDRTFERIASGREPFWARVQMEDRTDRVYFQNDRRAIYALGYPLLTPIGHLINLAELS
                     TLVGLVYAVLLVGGRLFRVLGLRRATSGLGLWREIRESFYRKLFLAFVGASIIPVVTL
                     ALVTRVYIGDQLRRDVEAAAQNTTAITRQMIEELSAVRGEADVLAALNDDALVSLSRV
                     VGQDVNLYRGPRLIASSERDLFASGLLSTRTPADVYRAILLDRLPTVITEEQAGEFRF
                     MVAGAPVRAVGPDALLTVPLALRQQEIEREIEELNRRIVLAALLFILVGAAFGYPLAE
                     RIADPVSRLTRATERLSRGDFDVRIATSASDELKRLVEAFNTMSAELRRQRVELERTH
                     RLEAWAEMARQVAHEIKNPLTPIQLSAEHLRRVHADRGRPLEPVLDGCVDSILSQVRL
                     LRQIASEFSSFASSPTARLAPTVLADLVDEVVRPYRSGGDRIEFAVEVPASLPALEID
                     RTLIGRALTNVIDNALHAMPGGGRLAIHAARVADGAVELVVQDNGVGMDEEALGRLFE
                     PYFSTKAVGTGLGLSIARRNVELNGGTIQVASERNVGTRVTITLPVAARAEEAD"
BASE COUNT          758 a         1928 c         1947 g          684 t
ORIGIN      
        1 cgcgcgaggc cgacgtggtg ctgtcgcccg ccgacggccg cgtcgtcatc gccggcgacc
       61 cgcagccggg cctgcccccg ccgggagcgt ggcgccaggt gagcatcttc ctgtcgccgc
      121 tcgacgtgca cgtcaaccgg atgccggttg cgggccgcgt ctcgcgcatc gagtaccggc
      181 cgggccgcta ccgcgcggcg tacgacgagg cggccggtgc cgagaacgag cgcaacgaga
      241 tctggatcga tcgcgacggc gggccggtcg tgttccgcca gatcaccggg gtcctggtgc
      301 gccgcgtggt gtgccgggcg cgcgtgggcg acgtggtggc cgccggcgac cggttcggcg
      361 tgatgaagtt cgggtcgcgg atcgacctct tcctgccggc ctcggcgcgg ctgctcgtgt
      421 cgcccggcga ccgggtgcga gccggtgaga ccgtcgtcgc ccggctggcc tgacagacca
      481 tgcaactcga gctggcccgc gatcaccgcc cccggcctcc gcacccgctg aagcggcgga
      541 ccgcggaaca gcggcgccgc ttccgccgcg gtgtgtacct gctcccgagc gtcttcacgc
      601 tcggcaacat gttctgcggc tacatctgca tcgtctacag catccggggc gacttcgaga
      661 cggcggcgcc gttcatcgcc ttctcggtcg tgctcgacat gctcgacggc cggatcgcgc
      721 ggctgaccgg cagcacgagc gccttcggcc tcgagttcga ctcgctggct gacgtcgtgt
      781 cgttcggcgt ggcgcctgcc atcctgacgg tgtcgtgggg attgacgccg ctcgggcggc
      841 tggggtgggc ggcggggttc atcttcgtga cggccggggc gattcggctg gcgcgcttca
      901 acatccaggc gagcaccgac aagcggcact tcgtggggat gccgatcccc gcggcggcgg
      961 cggtgccggc ctcgaccgtc tacgtgtacc cggccgggct gcaggacgtc ggcgccgcgc
     1021 tgcctgcgct ggccctgatg atcgtccccg cggtcctgat ggtgagcacc atccgcttcc
     1081 gcagcttcaa gtcgatcgac ctgcggtggc ggcgctcgtc ccgggtcttg ctgctgctgg
     1141 cgctgttcct gatggcgatc gccacgcacc cgcggtggac gctgctcgcc ctggcctaca
     1201 cctacctgct gtccgcgttc gtcgcgatgg cggcggcccg gcttcgccgc cgggcggcgc
     1261 ccgcgggcgc accgatcagt ccgcctcctc cgcccgagcc gccaccggca gggtgatcgt
     1321 cacccgcgtg ccgacgttcc gctcgctcgc gacctggatg gtcccgccgt tcagctcgac
     1381 gttcctccgc gcgatcgaaa ggccgaggcc ggtgccgacc gccttggtgg agaagtaggg
     1441 ctcgaagagg cggccgagcg cctcctcgtc catccccacg ccgttgtcct gcacgaccag
     1501 ctccaccgcc ccgtctgcaa cgcgggcggc gtgaatggcc aggcggccac cgccgggcat
     1561 cgcgtgcagc gcgttgtcga tcacgtttgt gagggcgcgc ccgatgagcg tgcggtcgat
     1621 ctcgagggcg gggagggacg cggggacttc gaccgcgaac tcgatgcgat cgccgccgct
     1681 gcggtacggc cgcacgacct cgtccaccag gtcggccagc accgtgggcg ccaggcgcgc
     1741 ggtgggggac gaggcgaagc tcgagaactc ggacgcgatc tgccgcagca gccgcacctg
     1801 cgacagaatc gaatccacgc agccgtcgag cacgggctcg agcggcctgc cccgatccgc
     1861 gtgcacgcgg cggaggtgct ctgccgagag ctggatcggg gtcagcgggt tcttgatctc
     1921 gtgcgcgacc tggcgcgcca tctcggccca ggcctcgagg cggtgcgtcc gctcgagttc
     1981 gacgcgctgc cggcgcagct cggccgacat cgtgttgaac gcctcgacca ggcgcttcag
     2041 ctcgtcggag gcgctggtgg cgatccgcac gtcgaagtcg ccccgcgaca gccgctcggt
     2101 cgccctcgtg agccggctga cggggtcggc gatccgctcg gcgagcgggt atccgaacgc
     2161 ggcgcccacc aggatgaaga gcagcgcggc cagcacgatc cgccggttca gctcctcgat
     2221 ctcgcgctcg atctcctgct ggcggagcgc gagcggcacg gtcagcagcg cgtcggggcc
     2281 gaccgcccga accggcgcgc cggccaccat gaaacggaac tcgccggcct gctcctcggt
     2341 gatcaccgtg ggcaggcgat cgagcaggat cgcccggtac acatcggcgg gagtgcgcgt
     2401 ggacagcagg cccgacgcga acaggtcccg ctcgctgctg gcgatgagcc gaggcccccg
     2461 gtacaggttg acgtcctgtc ccaccacccg gctgagcgac acgagcgcgt cgtcgttgag
     2521 cgcggccagc acgtcggcct cgccgcggac ggccgacagc tcctcgatca tctgccgcgt
     2581 gatggccgtc gtgttctgcg cggccgcctc gacatcgcgg cgcagctggt cgccgatata
     2641 gacgcgcgtc accagggcga gggtgacgac cgggatgatc gacgcgccaa cgaacgccag
     2701 gaacagcttc cggtagaagc tctcgcggat ctcgcgccac agccccagtc ccgaagtcgc
     2761 ccgccgcagg ccgagcacgc ggaacagccg gccgccgacg agcagcacgg cgtagacgag
     2821 gccgaccagc gtgctcagct cagccaggtt gatcaggtgc ccgatcggcg tgaggagcgg
     2881 gtagccgagc gcgtagatcg cgcgccggtc gttctggaag tacacgcggt cggtgcggtc
     2941 ctccatctgg acgcgcgccc agaagggctc tcggcccgag gcgatccgtt cgaaggtgcg
     3001 atcgtcgagc ggccacgcgc gggcgcccga aaggtagatc gcgcttcggc tccagccgta
     3061 gacgacgtac tcgatctcgc ggccgggcgc cgcctcgcgc ggcagcaggg cgccgccgcg
     3121 cagcagctcg aagtacgggt cctgggacga caggaacgac agcgtgctgt cgtcgaggat
     3181 cccgtggatg acgatggtgc cgagaatgcc gtcctcgccg cagatgttgc gtccggcatg
     3241 gagcagctgc cgctcctgcg cgccgaacgg gctgctctcg ccgaaggtgc gccactcgca
     3301 tccctcctcg cgccagcgct ggcggctcgc gtcctcgggg aggttgaggg cgaaccggct
     3361 caccagccac tgcccgccgt tgtacagctc gacggccgac gatacgcggt ggcgctcgag
     3421 cgccgtcccc tgccagacca ggaaggcgct gtcgggcggg atggggctcc cgggcggagg
     3481 cgcctcgacc gacaccaccg agcggaggac gtcgtcgcgc cggtcgatct cctgctgcgc
     3541 ggtctgcacg cgccgctgca ggttctcgag ctggtgcacc acctcggggg cgtaccggcc
     3601 ctcgatgacg cgccgcttcg ccacgtcctc gaagaacacg agcgacgggt agaagagcag
     3661 cgacggcagg agcagcgcga cgaacatcgc cgccaggcgg tacccctgcg aggcgtggcg
     3721 gagccgcgtg aggccgcgcg gggcatggaa cgcggcggcg gccagccccg ccgacgcgag
     3781 cagcgccccc gaccggggca gccccgaagt gccgccccac caggcccacc ccagcacgac
     3841 cggcgcgccc caggcgagcg cgagcagcgc caccgccctt ggctcggcgc ccaccagcca
     3901 gaaggcaagc gcgctgcgca ggcccagcac ggccgcccag gcgaacgccg cgtggaacag
     3961 caccaggccc gccccgagtg ccacccgccc ggcgttccac gggtggggcg agaagtagag
     4021 gatgtcggtg cccgacgagg cgaaggtgtc gcccaggaag cgctcgaaca gcgcgacggc
     4081 ggccaccagc gcgaggccgg ccacggcctg tgcggcgacg aagcgcgccg ccgacgcgcc
     4141 gcgcggcaac gacgtccggc gcgagcgccg cccgatgcgc cagcgctcgg cagggcccgc
     4201 gatcagcgcg accaacgcga tggccagcag cccggtggcc aggaagtcgg cggggtggcg
     4261 gaaccacgcg ccgagcgcgg cggacttgta gatcgccggc gagaacagcc gcgagccccc
     4321 aggcccgcgg atgagccaca gcaggccgcg cgcaacgagc aacagtaccg cgacggcaac
     4381 cgtcggaacg cagtactcgc cgcgtgtacg cgcgcgctgt cggccgtcga gcagagggcc
     4441 cgccagcagc agaacggtca gcgcggccgc cagccacgcc aggtccacca ccccgcgccg
     4501 gtggcgggcg cgggcctctg cgagatcctc gtatgcgacc gaggcttcga ccaggggccg
     4561 cccgccagcg tcgtggagca ggaaggcatg gcgcccggag ggttggtcgc gcgcaatctc
     4621 ggaggcgggc cgcagcgcga ccggcgcgag cggggtcggc atggtgggcg tcgagccggc
     4681 atgcccgggc atcccggaca cggtgctgag cagcgcctcg gcggcgattg ttccgaggag
     4741 cggccccccg ctccccggtc ccgtcaccgg cgcggtcgcc accagccgca cgcccactgg
     4801 cccgcgcgac acgaagaggc tcggcccccc gttcaggcgc tcgacgggaa cctgggaggg
     4861 gcgccccgcc cacgcgagcg gactgccatc ggtgccgtag atcgtgacgg cgatggttcg
     4921 cgcgtcgggg tcggaggtca gctcgtcggc gcggccgaac agcgggcgca ggtcgcgctc
     4981 gtcggccggg tgcgcgagcg agggtcgcgc cgcctccgcg aggtcgctcg cggctccgcg
     5041 caacgcgtgc gacacccgat cgagctgcgc gcggacgctg ccctccacgc gccgcgcggc
     5101 ggcccgatcg ttgggtccca aacggacgcg ttccacggcc cacccgccga tgacgacgag
     5161 gagggtcgcc gcaaggccgg ccacgaggat ggcgatcagt gattgtcggc gagaagcgag
     5221 ccgcatgcct tggcgtcagc atacgacaaa actgacaccc gccgtgctga aggtggagag
     5281 actgacgccc gtcgtgctga aggtggagag actgacg
//
LOCUS       DAOPQX010000252         5305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_16981_length_5305_cov_4.139048, whole genome shotgun sequence.
ACCESSION   DAOPQX010000252 DAOPQX010000000
VERSION     DAOPQX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16981_length_5305_cov_4.139048"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1774
                     /gene="nuoC"
                     /locus_tag="PKK95_12530"
     CDS             17..1774
                     /gene="nuoC"
                     /locus_tag="PKK95_12530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF008728.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HOC19092.1"
                     /translation="MPAAAPPTDVLSEVRTRFGEAGLVEEAALDNVPTVWVPPASAAE
                     VLGFLKRDVHQPYRMLFDLTAIDERVRRERNSLPASDLTVVYTLFSYERNEYVRVKAA
                     LPESRPSLPSIARLWPSANWYERETYDMFGVTFEGHPHLARILMPPTWEGYPLRKDHP
                     ARATEMGPYELPTEKRFAELEALRFDPAAWGMEERRDGVDFLFLNLGPQHPGTHGVFR
                     ILLQLDGEEIVDCVPDIGYHHRAAEKMGERQTWHTYIPYTDRVDYLGGVVNNLAYLTA
                     VERLAGIEAPPRAQVVRVMMSELFRIMSHLVWYGTFAQDVGQMSPVFYTFNDRERGYA
                     IIEAVTGFRMHPAWFRIGGLAADLPRGWERLFREFLSGFSRNLAEYNREIIRNEIFQA
                     RTRGIGGCSADEAIEWGMTGPNLRACGFEWDLRKRQPYSGYEQFEFDVPTGTTGDCFD
                     RAVVRIEEMRQSVRIIEQCVAHMPEGPIKADHPLTTPPPKDRTLHDIETLIAHFLHVS
                     WGPVMPPGEVLGVIEGTKGSYGYHLVSDGGTQSYRTRIRTASFPHMQMLPLLSRGLLV
                     PDLLAILGSLDYVLSDVDR"
     gene            1779..2243
                     /gene="nuoE"
                     /locus_tag="PKK95_12535"
     CDS             1779..2243
                     /gene="nuoE"
                     /locus_tag="PKK95_12535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOC19093.1"
                     /translation="MLSPDERREIEKELSRYPTRQAVCIDAMRIVQRHRGWVSDESLA
                     DIAELLGMSRHELDSVATFYNLIFRKKVGRHVIFVCDSVSCWVMGYERLYRALSERLG
                     IAFGETTPDGRFTLLPIVCLGACDRAPAMMVDGTLHGNLDPDRIDPILERYE"
     gene            2247..3518
                     /gene="nuoF"
                     /locus_tag="PKK95_12540"
     CDS             2247..3518
                     /gene="nuoF"
                     /locus_tag="PKK95_12540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOC19094.1"
                     /translation="MERPLTARVRADGEAVTLAEYERAGGYRGLRRALQESPADIGAT
                     VREANLRGRGGAGFNTGLKWSFVPLGPDAPHPKYVVCNADEMEPGTFKDRLLLEADPH
                     QLIEGLAIAAWAIEADVAYIFLRGEYTDAAARLERALAEAYAAGYLGPDVLKSGWRLE
                     MYLHLSAGRYICGEETALLNALEGKRANPRAKPPYPQVSGLWGKPTIVQNVETLCNLP
                     HILANGAEWFKSISRSPDGGTKLYGASGKVRRPGLWELPMGTPARELLEEHAGGMRDG
                     TSFRGLLPGGASTDFLLPEHLDVKMDFDEVQKAGSRLGTGTMIVLDDRTCPVGFVHNL
                     ERFFAQESCGWCTPCWSGLHWATEALRALEEGRGTPADLAALEHQTRFNAMGRTFCAL
                     APGAIEPLQSALAYFRDDFERHVRERRCPWK"
     gene            3525..>5305
                     /gene="nuoG"
                     /locus_tag="PKK95_12545"
     CDS             3525..>5305
                     /gene="nuoG"
                     /locus_tag="PKK95_12545"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOC19095.1"
                     /translation="MARIHVDGRAYEVDPSRNLLDACLSHGLDLPYFCWHPAMGSVGA
                     CRQCAVKQFRDEHDAKGRIVMACMTPASDGTRISIADAEAAAFRASVIEGLMESHPHD
                     CPVCDEGGECHLQDMTVMTGHTLRRYPYSKRTFRNQYLGPFIAHEMNRCIQCYRCVRF
                     YRDYAGGHDLDVFGIRNGVYFGRAEDGVLESEFSGNLVDLCPTGVFTDLTLGVRYTRK
                     WDLQTAPSICMQCSLGCNITAEERYGSLRRIVNRYHRDVNGHFICDRGRFSHGFVNSP
                     GRIREALVRRDGGLVAVPPGEALQQAAALLSGRRVIGLGSPRASLEANFALRALVGPD
                     HFYQGVPDAQGRLVGLMLDILRQGPVRTPAPAEVEEADAVLVIGEDFAATGVRMALSL
                     RQAVRRPGFELAARMKVPLWQDAAVRELQQELRGPLFILSTCPTGLDDVAEVTQRAAP
                     QDLARLAYAVGRRLDERVAGPADLPEPLAALASSMADTLARARRPLVVSGASSGSAEV
                     IEAAAAVASALQARGAAASLVFLAPEANSVGLTLLGPRPFGEAARLAGEGGVDAVVVL
                     ENDLSWRFAPGEGQALLVSARAVPRNSA"
BASE COUNT          776 a         1916 c         1846 g          767 t
ORIGIN      
        1 cccgacgaga tctgagatgc ccgccgccgc ccccccaacc gacgtgctct cggaggtgcg
       61 cacccggttc ggcgaggccg gcctggtcga ggaggccgcc ctcgacaacg tgccgaccgt
      121 ctgggtgccg ccagcatcgg ccgcggaggt gctcgggttc ctgaagcgcg acgtccacca
      181 gccctaccgt atgctcttcg acctgacggc gatcgacgag cgcgtccgcc gcgagcggaa
      241 cagtctgccg gcgagcgacc tcacggtggt ctacaccctg ttctcgtacg agcggaacga
      301 atacgtgcgc gtcaaggcgg cgctgcccga gtcgcggccg tcgctcccgt cgatcgcgcg
      361 tctctggccc tcggcaaact ggtacgagcg cgagacgtac gacatgttcg gggtcacctt
      421 cgagggacat ccccacctcg cgcgcatcct gatgccgccc acgtgggagg gctacccgct
      481 ccggaaggac cacccggctc gcgcgaccga gatgggcccc tacgagctgc cgaccgagaa
      541 acggttcgcc gagctcgagg cgcttcgctt cgatccggcc gcctggggca tggaggagag
      601 gcgcgacggg gtcgacttcc tgttcctcaa cctcggaccg cagcatcccg gcacccacgg
      661 ggtcttccgg atcctcctcc aactcgacgg cgaggagatc gtggactgcg tccccgacat
      721 cggctaccac caccgggccg ccgagaagat gggcgagcgc cagacctggc acacctacat
      781 cccctacacc gaccgggtgg actacctggg cggcgtagtg aacaacctcg cctacctcac
      841 cgcggtggag cgcctcgcgg ggatcgaggc gccgccgcgc gcgcaggtgg tgcgcgtcat
      901 gatgtcggag ctgtttcgca tcatgagcca cctcgtctgg tacggcacct tcgcccagga
      961 cgtcggccag atgtcgccgg tcttctacac cttcaacgac cgcgagcgcg gctacgcgat
     1021 catcgaggcg gtgaccggct tccgcatgca ccccgcctgg ttccgcatcg gcggtctggc
     1081 cgccgacctg ccgcggggat gggagcgcct gttccgcgag ttcctgtcgg gcttctcgcg
     1141 caacctggcc gagtacaacc gcgagatcat ccggaacgag atcttccagg cgcgtacgcg
     1201 cggcatcggc ggctgctctg cagacgaggc gatcgagtgg gggatgacgg ggccgaacct
     1261 gcgggcctgc ggcttcgagt gggacttgcg caagcggcag ccgtactccg gctacgagca
     1321 gttcgagttc gacgtcccca ccggcacgac cggcgactgc ttcgatcggg cggtcgtccg
     1381 catcgaggag atgcgccaga gcgtgcggat catcgagcag tgcgtcgcgc acatgcccga
     1441 ggggccgatc aaggccgacc acccgctcac gacgccgccg cccaaggacc gcacgctgca
     1501 cgacatcgag acgctcatcg cccacttcct gcacgtgagc tggggccccg tcatgccgcc
     1561 cggcgaggtg ctgggagtga tcgaggggac caagggcagc tacgggtacc acctggtcag
     1621 cgacggcggc acccagtcgt accggacccg gatccgcacg gcgtcgttcc cgcacatgca
     1681 gatgctcccg ctgctgtcgc ggggcctgct cgtgcccgac ctgctggcga tcctcgggag
     1741 cctggactac gtcctgtcgg acgtcgatcg atgacgagat gctgagccct gacgaacggc
     1801 gcgagatcga gaaggagctg tcccgctacc ccaccaggca ggcggtctgc atcgacgcga
     1861 tgcgcatcgt ccagcgccac cgcgggtggg tctccgacga gagcctggcc gacatcgccg
     1921 agctgctcgg gatgtcgcgc cacgagctcg actcggtggc gaccttctac aacctgatct
     1981 tccgcaagaa ggtcggccgc cacgtgatct tcgtgtgcga cagcgtcagc tgctgggtga
     2041 tgggctacga acggctgtac cgcgcgctct ccgagcgcct gggcatcgcc ttcggcgaga
     2101 cgacgccgga cggccgtttc acgctgctgc cgatcgtctg tctcggggcg tgcgaccgcg
     2161 cgccggccat gatggtggac ggcaccctgc acggcaacct cgaccccgat cggatcgacc
     2221 cgatcctcga gcgctacgag tagaccatgg agcgaccgct gaccgcgcgc gtccgggccg
     2281 acggcgaggc cgtcaccctc gccgagtacg agcgggcggg cggctaccgc ggactgcgcc
     2341 gcgcgctgca ggagtcgccg gccgacattg gagcgacggt gcgcgaggcg aacctgcgcg
     2401 gccgcggcgg ggccgggttc aacacgggcc tgaagtggag cttcgttccc ctgggccccg
     2461 acgccccgca ccccaagtac gtcgtctgca acgcggacga gatggaaccg ggaaccttca
     2521 aggaccgtct gctgctcgag gccgacccgc accagctgat cgaggggctt gccatcgcgg
     2581 cgtgggccat cgaggccgac gtggcctaca tcttcctccg cggcgagtac accgacgcgg
     2641 cggcgcgact ggagcgggcg ctggccgagg cgtacgcggc tggctacctc ggccccgacg
     2701 tgctgaagtc gggctggcgg ctcgagatgt acctgcacct cagcgccggc cgctacatct
     2761 gcggcgagga gacggcgctg ctgaacgccc tcgaggggaa gcgcgccaac ccgcgcgcca
     2821 agccgcccta cccgcaggtc tcggggctgt ggggcaagcc gaccatcgtg cagaacgtcg
     2881 agacgctctg caacctgccc cacatcctgg cgaacggggc cgagtggttc aagtcgatca
     2941 gccgcagccc ggacgggggc accaagctgt acggcgcgag cggcaaggtg cggcgcccgg
     3001 ggctgtggga gctgccgatg gggacgccgg cgcgcgagct gctcgaggag cacgcgggcg
     3061 gcatgcgcga cgggacctcg ttccgcggcc tgctgcccgg cggcgcctcc acggacttcc
     3121 tcctccccga gcacctcgac gtgaagatgg acttcgacga ggtgcagaag gccgggagcc
     3181 ggctcggcac tggcacgatg atcgtcctcg acgaccgcac ctgcccggtg ggcttcgtcc
     3241 acaacctcga gcggttcttc gcccaggaat cctgcgggtg gtgcacgccg tgctggagcg
     3301 ggctgcactg ggcgaccgaa gcgctgcgcg cgctcgagga ggggcgcggc acgccggccg
     3361 acctggccgc cctcgagcac cagacccggt tcaacgcgat gggccgcacc ttctgcgcgc
     3421 tggcgccggg tgcgatcgaa cccctccaga gcgccctcgc gtacttccgc gacgacttcg
     3481 agcggcacgt gcgcgagcgt cgctgcccgt ggaagtgagg cgcgatggcg aggattcacg
     3541 ttgacggacg ggcctacgag gtcgacccct cccgcaacct gctcgacgcg tgcctctcgc
     3601 acggcctcga cctgccgtac ttctgctggc acccggcgat gggatcggtc ggcgcgtgcc
     3661 gccagtgcgc ggtgaagcag ttccgcgacg agcacgacgc gaagggccgg atcgtcatgg
     3721 cctgcatgac gccggcgagc gacggcacgc gcatctcgat cgccgacgcc gaggcggcgg
     3781 cgttccgggc gtcggtcatc gaggggctga tggagagcca cccgcacgac tgcccggtct
     3841 gcgacgaagg gggcgagtgc cacctgcagg acatgacggt gatgaccggg cacacgctcc
     3901 gccgctaccc gtactcgaag cgaaccttcc gcaaccagta cctgggcccg ttcatcgcgc
     3961 acgagatgaa ccgctgcatc cagtgctacc ggtgcgtccg gttctaccgc gactacgcgg
     4021 gcgggcacga cctcgacgtc ttcggcatcc gcaacggcgt gtacttcggc cgggcggagg
     4081 acggcgtcct cgagagcgag ttcagcggga acctggtgga cctctgcccc accggcgtgt
     4141 tcaccgacct cacgctcggg gtccgctaca cgcgcaagtg ggatctccag accgcgccgt
     4201 ccatctgcat gcagtgcagc ctcggctgca acatcacggc cgaggagcgc tacggcagcc
     4261 ttcgccgcat cgtcaaccgc taccaccgcg acgtcaacgg ccacttcatc tgcgatcgcg
     4321 gccgcttctc acacggcttc gtcaactcgc ccgggcgcat ccgcgaggcg ctggttcgcc
     4381 gggacggcgg cctggttgcc gttccgccgg gcgaggcgct gcagcaggcc gccgcacttc
     4441 tctccggccg gcgcgtcatc ggcctgggct cgccgcgcgc ctcgctcgag gccaacttcg
     4501 cgctgcgcgc gctggtcggc cccgaccact tctaccaggg ggtgcccgat gcgcagggtc
     4561 gactggtcgg cctgatgctc gacattctcc gccaggggcc tgtgcgaacc ccggcgcccg
     4621 ccgaggtcga ggaggccgac gcggtgctgg tgatcggcga ggacttcgcc gcgaccggcg
     4681 tgcggatggc cctgtcgctg cggcaggcgg ttcgtcggcc gggattcgag ctggccgcgc
     4741 gcatgaaggt cccgctgtgg caggacgccg ccgttcgcga gctgcagcag gagctccgcg
     4801 gcccgctctt catcctcagc acctgcccga cgggcctcga cgacgtggcg gaagtcaccc
     4861 aacgggccgc cccgcaggac ctggcgcggc tggcctacgc cgtcggccgc cgcctggacg
     4921 agcgcgtcgc cggcccggcc gatttgcccg aacccctggc cgccctcgcg agctcgatgg
     4981 ccgacacgct cgcccgcgcc cgccggcccc tggtcgtgtc gggcgccagc tcggggagcg
     5041 ccgaggtgat cgaggcggcc gccgccgtcg cctccgccct gcaggctcgc ggcgccgcgg
     5101 cgtcgcttgt ctttctcgcg cccgaggcca acagcgtcgg cctgacgctg ctcggcccgc
     5161 ggcccttcgg cgaggcggca cgcctggccg gtgaaggggg ggtggatgcg gtcgtcgtgc
     5221 tggagaacga cctgtcctgg cgctttgcgc cgggcgaagg gcaggcgctg ctcgtcagcg
     5281 cccgcgcggt gccgcgcaac tcggc
//
LOCUS       DAOPQX010000253         5251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17184_length_5251_cov_4.677829, whole genome shotgun sequence.
ACCESSION   DAOPQX010000253 DAOPQX010000000
VERSION     DAOPQX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17184_length_5251_cov_4.677829"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKK95_12550"
     CDS             <1..314
                     /locus_tag="PKK95_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19096.1"
                     /translation="RLLVFDSPDHSRMTGYQVGFFLPGAAAAAGIVNLGPDAFLERLA
                     FDLPPWEIEAMGRTLVAPIASAGLSTPPGRAFTYRVRGVWAGGTTAWSGPSDPFVRCP
                     Q"
     gene            complement(449..3148)
                     /locus_tag="PKK95_12555"
     CDS             complement(449..3148)
                     /locus_tag="PKK95_12555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC19097.1"
                     /translation="MPVPAPGPPPDRPLISRAVASPPAEIPFPATLAPSATWPLTEQR
                     VLVLALAAIVLLAATFRVQGLDYGGFSEDEVNKVAALRAYAAGNFTANAEHPMLMKLA
                     MLGATVAADAWNYLATRTGWHAVSPEAALRLPNALVGAATVIPLFLLARSFFGPLVAL
                     WASLLLALDVTVTGLNRIGKEDTFLVFFLLLGTWLYEEARARHAPGGTVPCRWYAASG
                     AAFGLMLASKYMIYYLGLWALFRLAAGAEERKLAGPRPAPAGPPRRAPRAFYAAAFVA
                     FVIANPAILSPATWAYCLHYASGGTMIHHGAYFAGQIYPNAITDTPWGLPWHFYLLYL
                     AVKIPLPILAAIGVGVAELVRLRRQRGAVFARVFLLFFLLPSSLAAAKFARYLLPALV
                     VLQLVAALGVVRALDLVVRWRGLRLRVAAAGAIVALVVVTALNAQVTAAPFASLHLNA
                     IGHRLSAPGGLFPNDELYDIGVREAVLWIAGRAAPGASVASDAPGVVGEYLRRAGRTD
                     IEARPLSTEGLPAPPAEVWLLAQSSHACFESQPTVDLVRRRERPVFVYRVHGTAAVEV
                     FHLDWRSPAGLAPNASRALTSRGQQHGARLRPAAWAIDGTPARGPAVLRAARYSLAGR
                     VRPLLFWVGKDNVGEARIAWLAGSGEAPGGYELLIGSDPARAPRKINRWGYLAETEGE
                     GEVQVVGLMTEVDHETLEQAKASVDGPAKGSRAYKVIHGLVSPSDAGTTVRGVMLPES
                     FTFRDLAAAMEWLPTAGMPERQIAVPPGAEPGFLHAMASLLREHVETFRQRGVAAAGG
                     SRTYVYGGKVYDVTIRSSKALARAAYQGVEYRDLIESEFQAKARSGGGGSKFKLAYGT
                     EGPLREVPIRIVYRPKWWFEAEMVLVEGPGLEVPFLARRTTSR"
     gene            complement(3263..4396)
                     /locus_tag="PKK95_12560"
     CDS             complement(3263..4396)
                     /locus_tag="PKK95_12560"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC19098.1"
                     /translation="MLRAAFAAALATAAVIAQSPAPPPPRGLRTLDLSADTARQVIVD
                     REPGQYLGHPTTVLLEDTRTMLVVYPKGHGRGPIVMKRSEDGGRTWSGRLEVPASWAT
                     SQETPTLHRVVDARGAKRLILFSGLYPIRMSVSEDDGRTWSELKPIGDFGGIVAMASV
                     VPVRTGPGHYLAFFHDDGRFFTAKGAPTTPPTFTVYQSRSIDGGLTWGAPAVVWSGQD
                     LHLCEPGAIRSPDGARLALLLRENSRRRNSHAVFSGDEGKTWSRPVELPASLTGDRHV
                     GRYAPDGRLFVSFRDMAANSPTQGDWVAWIGTWDDIASGREGQYRVRLADNHNRWDST
                     YPGVEVLPDGTIVTTTYGHWTPGEQPYILSVRLKLPELDAMTR"
     gene            complement(4398..4964)
                     /locus_tag="PKK95_12565"
     CDS             complement(4398..4964)
                     /locus_tag="PKK95_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOC19099.1"
                     /translation="MKRFAVLLLFAAIGLAPATAHAQKYFGGFTGYSYGGAAGECPSL
                     WDDCPNRRTGYGIVLGGLGGGFGIEQEIAWTPDFFGEEGEVADSNVLTVMSNLVFSIP
                     VGPLRPYGTAGLGFIKAKAEFTTESLTDFSDTAFGWNYGAGVMVMLPAHLGVRLDYRR
                     IRSGPEVSFLGGDDKVEFSRVTLGVVLH"
     gene            5047..>5251
                     /locus_tag="PKK95_12570"
     CDS             5047..>5251
                     /locus_tag="PKK95_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19100.1"
                     /translation="MMSFRPRLARETRLLLVTVVLSAGTLLVLARFRFPDAPPPALPR
                     EQPLQRLAAEALFDELAATVALAR"
BASE COUNT          809 a         1865 c         1828 g          749 t
ORIGIN      
        1 ctcggctgct ggtcttcgac tcgcccgatc actcgcgcat gacgggctac caggtggggt
       61 tcttccttcc cggcgcagcg gccgccgcgg gcatcgtgaa cctgggtccc gatgccttcc
      121 tcgagcggct cgcgttcgac ctccccccgt gggagatcga ggcgatggga cgcacgctcg
      181 tcgcgccgat cgcctccgcc ggcctctcga cacctcccgg ccgcgccttc acctaccggg
      241 tgcgcggcgt gtgggcgggg ggaacgaccg cctggtccgg cccgagcgac cccttcgtgc
      301 gctgcccaca gtaaggtcct ccgcgacgac gaaggaacca caaaggacac aaaggaggag
      361 cggctgcctt gcggcggccg ccgaagaccg gccccagcgg acgctctttt tgatctgtgg
      421 ttcagccgtc ctggggcctg ccgcctcctt agcggctggt ggtgcgtcgc gcgaggaacg
      481 gcacctcgag acccgggccc tcgaccagca ccatctccgc ctcgaaccac cacttcggcc
      541 ggtacacgat tcgaatgggc acctcccgca aggggccctc ggtgccgtac gcgagcttga
      601 acttcgaccc gccgcccccc gaacgggcct tggcctggaa ctcgctctcg atcagatcgc
      661 gatactccac gccttggtac gccgcacgcg cgagcgcctt cgacgatcga atcgtcacgt
      721 cgtacacctt gccgccgtag acgtacgtgc ggctgccgcc cgccgccgca acgccgcgct
      781 gccggaacgt ctccacgtgc tcgcgcagca gcgaggccat cgcgtgcagg aagccgggct
      841 cggcgccggg cgggacggca atctgccgtt ccggcatccc ggcggtgggc agccactcca
      901 tcgccgccgc gagatccctg aaggtgaagc tctcgggcag catgaccccc cggaccgtcg
      961 ttcccgcatc ggacggcgag acgagcccgt gaatcacctt gtacgcgcgc gatcccttgg
     1021 ccggcccgtc gacgctggcc ttggcctgtt cgagcgtctc gtgatccacc tctgtcatca
     1081 ggccgacaac ctggacctcg ccctcgccct ctgtctcggc gaggtacccc cacctgttga
     1141 tctttcgcgg ggcgcgcgcg ggatccgacc cgatgagcag ctcgtatccg cccggtgcct
     1201 cgcccgaacc cgcaagccac gcgattcgcg cctcgccgac gttgtccttc cccacccaga
     1261 agagtagcgg tcgaacgcgg cctgccagcg aatagcgagc cgcccggagc accgcgggcc
     1321 cgcgcgccgg cgtcccgtcg atcgcccacg ctgccgggcg aaggcgggcg ccgtgctgct
     1381 gaccgcgaga ggtcaaggcc cgcgaggcgt tgggcgccag gcccgccggg gagcgccagt
     1441 ccagatggaa cacttccacc gccgccgtcc cgtggacgcg gtacacgaag accggccgct
     1501 cgcggcgacg gacgagatcc accgtcggct ggctctcgaa gcaggcgtgg ctgctctgcg
     1561 cgagcagcca cacctcggcc gggggcgccg gcaggccctc ggtcgaaagg ggtctcgcct
     1621 cgatgtcggt ccgtcccgcc cgccgcaggt actcgccgac gacgccgggc gcgtcgctcg
     1681 cgaccgaggc gcccggtgct gcccgcccgg caatccagag cacagcctcg cggacgccga
     1741 tgtcgtacag ctcgtcgttg gggaagagcc cgccgggcgc cgaaagacga tggccgatcg
     1801 cgttcaagtg cagcgacgcg aaaggagccg cggtcacctg cgcgttcagc gcagtcacca
     1861 cgaccagcgc cacgatggcg ccggccgctg ctacccgcag gcgcagcccc cgccaacgga
     1921 ccaccaagtc gagcgcccga accaccccga gtgcggcgac cagctgcagc acgacgaggg
     1981 ccggcagcag gtagcgggcg aacttggccg ccgccagcga agagggcagc aggaagaaca
     2041 ggaggaacac gcgtgcgaag acggcgcctc gctgccggcg gaggcgcacc agctcggcca
     2101 ccccgacgcc gatggcggcc aggatcggca gcgggatctt gacggcgagg tacaggaggt
     2161 agaagtgcca gggcaggccc cacggcgtgt cggtgatcgc gttcgggtag atctgtccgg
     2221 cgaagtacgc gccgtggtgg atcatcgtcc cgccgctggc gtagtgcagg cagtaggccc
     2281 aggtggcggg cgacaggatc gccgggttgg cgatgacgaa cgccacgaag gcggcggcat
     2341 agaacgcgcg cggggcgcgc cgcggcgggc cggccggtgc cggccggggc cccgcgagct
     2401 tccgctcctc ggcgccggcc gcgaggcgga acagcgccca caggcccagg tagtaaatca
     2461 tgtacttcga cgccagcatc aggccaaagg cggcgccgct tgccgcgtac cagcggcacg
     2521 gaaccgttcc ccccggcgcg tggcgcgcgc gggcttcctc gtagagccag gtgccgagca
     2581 gcaggaagaa caccaggaag gtgtcctcct tcccgatccg gttcaatccc gtcaccgtca
     2641 cgtcgagggc cagcagcagc gaggcccaga gcgccacgag cggcccgaag aaggagcgcg
     2701 cgagcaggaa gagcgggata accgtcgccg ccccaaccag cgcgttcggc aggcggagcg
     2761 ctgcctcggg cgaaacggcg tgccagcccg tccgagtggc caggtagttc caggcgtcgg
     2821 cggccacggt cgcgccgagc atcgcgagct tcatcagcat cgggtgctcc gcgttggccg
     2881 tgaagttgcc agcggcgtag gcacgcagcg cggcaacctt gttgacctcg tcctcgctga
     2941 acccgccgta gtcgagtccc tggacgcgga aggtcgccgc cagcagcacg atcgccgcga
     3001 gcgcgagcac gagcacccgt tgttcggtca gcggccacgt agcgcttggg gcaagagtgg
     3061 cggggaacgg gatctcagcc ggcggcgacg cgacagctcg gctgatcagg ggacggtcgg
     3121 gcggcggacc gggggccgga acgggcatcg gggggcctcg aacgaagcga agcggtcgcg
     3181 gacctcctcg tcttactggc ggttggggtg agcccaaccg ccattctacc ttgaagttcc
     3241 atgccaggaa ggctgggtct ggctaccggg tcatcgcgtc gagttccggc aacttcaacc
     3301 gtacggagag gatgtagggc tgctcaccgg gggtccagtg gccgtaggtc gtcgtgacga
     3361 tcgttccgtc cggcaacacc tcgacgcccg gataggtcga gtcccagcgg ttgtggttgt
     3421 ctgcgagccg tacgcggtac tgcccttcac ggcccgaggc tatgtcgtcc caggtgccaa
     3481 tccacgccac ccagtcgccc tgcgtcggac tgttcgcggc catgtcccgg aacgagacga
     3541 aaaggcgtcc gtcgggagcg taccggccga cgtgccggtc gccggtgagc gaggcgggca
     3601 gctcgacggg ccggctccac gtcttcccct cgtcgccgct gaagacggcg tgcgagttac
     3661 ggcgacgcga attctcccgc agcagcagcg ccaggcgcgc cccgtcaggc gaccggattg
     3721 ccccgggctc gcacaggtga aggtcctgcc cgctccagac gaccgccggc gcgccccacg
     3781 tcagcccgcc atcgatcgag cgcgactggt agacggtgaa ggtgggcggc gtggtcggtg
     3841 ccccttttgc cgtgaagaac cggccgtcgt cgtggaagaa ggccaggtag tgccccgggc
     3901 cggttcgtac cgggaccacg ctggccatcg cgacgatgcc gccgaagtcg ccgatcggct
     3961 tcagctcgct ccaggtgcgg ccgtcatcct cggagaccga catgcggatg ggatagagcc
     4021 ccgagaagag gatcaggcgc ttggccccgc gcgcgtccac cacgcggtgg agggtcggcg
     4081 tctcctggct ggtcgcccag ctcgccggga cctccagccg cccgctccag gtgcggccgc
     4141 cgtcctcgct gcgcttcatg acgatcgggc cgcggccgtg gcccttcggg tagacgacga
     4201 gcatcgtccg ggtgtcctcg agcagcaccg tggtcgggtg gccgaggtac tgtcccggtt
     4261 cgcggtcgac gatcacctgg cgcgcggtgt cggccgagag gtcgagggtc cgcaacccgc
     4321 gtggcggcgg cggggcaggt gactgcgcga tgacggcagc cgttgcgagg gccgcagcga
     4381 atgcagctcg cagcatgtca gtgcaggacc acgcccagcg tgacgcgcga gaactccacc
     4441 ttgtcgtcgc cgccgaggaa cgagacctcg gggcccgagc ggatccgccg gtagtcgaga
     4501 cgcacgccga gatgcgccgg gagcatgacc atcacgccgg cgccgtagtt ccagccgaac
     4561 gcggtgtcgc tgaagtcggt gaggctctcg gtggtgaact cggccttcgc cttgatgaac
     4621 ccgaggcccg ccgttccgta cggccgcagg gggcccaccg ggatgctgaa caccaggttc
     4681 gacatcacgg tgaggacgtt gctgtctgcg acctctcctt cctcgccgaa gaagtcgggc
     4741 gtccaggcga tctcctgctc gatcccgaag ccgcctccca ggccgccgag cacgatgccg
     4801 tagccggtgc gccggttcgg acagtcgtcc cagagcgacg ggcattcccc ggcagcgcct
     4861 ccgtacgaat agccggtgaa tccgccgaag tacttctgtg cgtgcgcggt ggcgggcgcg
     4921 aggccgattg ccgcgaaaag gagcaggact gcgaagcgct tcatgttccc cccgtgccca
     4981 gaagtataga tcccttgcca gctgcggggc cggccgtgaa tgtgagaaga tcgcggccat
     5041 ccgcgcatga tgtcctttcg accccgtctc gcgcgcgaaa cgcggctcct gctggtcacc
     5101 gtggtgctct ccgcgggaac gctgctggtg ctggcgcgct tccgctttcc tgacgcgccg
     5161 ccgcctgcac tgccgcgcga gcagccgttg cagcgccttg ccgccgaggc cttgttcgac
     5221 gaactggccg cgacggttgc tctcgcccgg t
//
LOCUS       DAOPQX010000254         5243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17214_length_5243_cov_3.557826, whole genome shotgun sequence.
ACCESSION   DAOPQX010000254 DAOPQX010000000
VERSION     DAOPQX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17214_length_5243_cov_3.557826"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKK95_12575"
     CDS             complement(<1..406)
                     /locus_tag="PKK95_12575"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOC19101.1"
                     /translation="MKPLLLALTLAASLAAGLAADQGMWTFDNFPRKAVAEKYGVQIT
                     DEWLGRVQKAIVRLETGCTGSFASPDGLILTNNHCVRSCLAENSTAKRDLVGGGFSAD
                     SRAAEIRCQGEQASVLVETVNVTDQMTRALAGI"
     gene            523..1122
                     /locus_tag="PKK95_12580"
     CDS             523..1122
                     /locus_tag="PKK95_12580"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOC19102.1"
                     /translation="MNIVWLLLIGAAVITAVFKGTMAEVSNSVFSSATTAIELAIGLV
                     GGMTLFLGLMRVAQDSGLVQVLARALRPVFRFLFPDVPKDHPALGAIAMNFGANMLGL
                     GDAATPFGLKAMQDLQTLNPDKESATDAMAMFVTLHSSSLQLIPVMVISLRAAAGSKN
                     PSEIIVATIFATAASMAVAIATSKLFARWYARRWQGGAA"
     gene            1119..1655
                     /locus_tag="PKK95_12585"
     CDS             1119..1655
                     /locus_tag="PKK95_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOC19103.1"
                     /translation="MIRDLVNAASSWIVALLILGVPLYAYAAKRIRVYESFIEGAKEG
                     FEIGVRIMPYLVAILVAIGMFRASGAMDWLVWILQPMVAWAGFPVEALPSSLMRSLSG
                     SAAFAMSSEIFKIYGPDSFVGRLVSVIQGSTETTFYIMAVYYGCVAVRKTRYTLAASL
                     ITDVSGIVAAFIICSLLF"
     gene            1686..2600
                     /locus_tag="PKK95_12590"
     CDS             1686..2600
                     /locus_tag="PKK95_12590"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOC19104.1"
                     /translation="MKPGDRVAMVAPASPPSGRRLLLAGKKRLEQLGLEVVAGRHVMS
                     EYGYLAGPDRERRQDLESAFRDPRIKAIFCARGGYGVSRLLSGFDAGLARRYPKALVG
                     FSDITVLHLALQKAGVVSFWGPMPCTGLGWTPFSVRGLQRVLMSAGPAGRLPFSAHRR
                     PRTLRRGVGEGPLTGGTLSLMAASLGTPYEIETRGRIVFMEDVDEEPYRVDRLLAQLI
                     AAGKLADAAGLALGIFTGTDVRNSPGKRSLTMTQVFADHLLPLKVPVLVNLAVGHVPD
                     QVTLPYGARARLDAGAKTLELLEPGVGD"
     gene            2660..5140
                     /locus_tag="PKK95_12595"
     CDS             2660..5140
                     /locus_tag="PKK95_12595"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOC19105.1"
                     /translation="MKTRCQIALSIALVLLASRAFGAPAVGFRAAKPVWPEGRATEMN
                     LLVGFRAVIPAPSAGEKVVLRVAASTLYRASVNGTFVGHGPARAAHGFYRVDEWDVTA
                     ALGRGPAVVSLEVAGYNANSYYLLDQPSFLLAEVTAGDTVLAATRAGATKSGSRKTGN
                     AERPVPFDAAILDHRVQKVQRYSFQRPFIEVYRLAPGFDRWLREPAAPFRVIEVEEVS
                     PKTLLPRRVPYPDFAVTRPVAHVAEGRVEKRAQVERLWKDRALTNIGPKLKGFPQGEL
                     ERVVSTEMQHLGSTQPASLNRKWREGDEAALAAGHYRILDLGQNLTGFIGMTVEVAAR
                     SELRLAFDEVLTGGDVDFKRLGTVSVVSYSLEPGTYRLETLEPYTLRYLKAINLEGEC
                     RLRDVYLRELSHPAVAGARFAASDERLNRLFEAGVSTFRQNTLDVFMDCPSRERAGWL
                     CDSFFTARSAFSLTGLPAVEDAFFENYLRAARFEHLPDGMLPMCYPADHYDGVFIPNW
                     AMWFVVQLEEYVSRGGDREVVEGLRARVLRLLDFLATYENADGLLEKLPSWVFVEWSE
                     ANKFVQDVNYPSNMLYAQALDVAGRLYGMPGLSAKAERVRATVRRQSFDGEFFVDNAV
                     RRPDGTLQVTRNRTEVCQYFAFFFGVADTTGDAALWEKLRGRFGPDRKETRAFPEIHP
                     ANSFIGNMLRMELLSRAGRSQQILEESIGYLLYMVERTGTLWENVGAEASLNHGFASH
                     IVRTLQRDVLGVHAVDPLRKVITLRFGDAKLAWCEGTVPTPDGPVRLRWWRDGGALDY
                     RVEVPAGYRVEVGGRDPFIGSCSTGAAG"
BASE COUNT          761 a         1750 c         1882 g          850 t
ORIGIN      
        1 cgatgcccgc cagtgccctg gtcatctggt cggtgacgtt cacggtttcg accagcaccg
       61 aggcctgctc gccctggcag cggatctccg cggcgcgcga gtcggcgctg aagccgccgc
      121 cgaccaggtc gcgcttggcc gtcgagttct cggccaggca cgaccggacg cagtggttgt
      181 tggtgaggat caggccgtcg ggcgaggcga acgagccggt gcacccggtc tcgagccgga
      241 cgatcgcctt ctgcacgcga ccgagccact cgtcggtgat ctggacgccg tacttctcgg
      301 cgaccgcctt ccgggggaag ttgtcgaagg tccacatgcc ttggtcggcg gccaggcccg
      361 ccgccaacga cgcggcgagg gtgagggcga ggaggagggg cttcatgaaa acaggatata
      421 ggggccaggg gccggggggc taggggctgg ggtcaagggc ccgaggtgag gggcgaagac
      481 gggcaacggc gggccgggtt gccgaggtag aatctggcgc ctatgaacat cgtctggctg
      541 ctgctcatcg gcgcggccgt catcaccgcc gtcttcaagg gaaccatggc cgaggtgtcg
      601 aactcggtct tcagctcggc cacgaccgcc atcgagctgg ccatcggcct ggtggggggc
      661 atgacgctct tcctggggct gatgcgggta gcgcaggaca gcggcctggt gcaggtgctg
      721 gcccgcgcgc tgcgcccggt gttccggttc ctcttccccg acgttccgaa ggaccacccg
      781 gcgctcggcg cgatcgcgat gaacttcggc gcgaacatgc tcggcctcgg cgacgccgcc
      841 accccgttcg ggctgaaggc catgcaggac ctgcagacgc tcaaccccga caaggagagc
      901 gcgaccgacg cgatggcgat gttcgtcacc ctgcactcca gctcgctgca gctcatcccg
      961 gtgatggtca tcagcctgcg tgcggcggcc ggctcgaaga acccgtccga gatcatcgtg
     1021 gcgacgatct tcgcgaccgc ggcgtcgatg gccgtcgcga tcgcgacgtc gaagctgttc
     1081 gcccgctggt acgcccgccg gtggcaggga ggcgccgcat gatccgcgac ctggtgaacg
     1141 cggcgtcgag ctggatcgtc gcgctgctca ttttgggcgt gccgctctac gcctacgcgg
     1201 caaagcggat ccgcgtctac gagagcttca tcgagggggc gaaggagggg ttcgagatcg
     1261 gcgtccgcat catgccgtac ctcgtcgcca tcctcgtggc catcgggatg ttccgggcga
     1321 gcggcgcgat ggactggctt gtgtggattc tccagccgat ggtggcgtgg gccggcttcc
     1381 cggtggaggc gctgccttcg tcgctgatgc gctcgctgtc cggatcggcg gccttcgcca
     1441 tgagctcgga gatcttcaag atctacggcc ccgactcgtt cgtcggccgg ctggtgagcg
     1501 tcatccaggg gagcaccgag acgaccttct acatcatggc cgtctactac gggtgcgtcg
     1561 cggtgcgcaa gacgcgctat acgctggcgg ccagcctgat caccgacgtg tcggggatcg
     1621 tggcggcgtt catcatctgc agcctgctgt tctgaaaggt gatgcgcttg atacttcctc
     1681 cgaggttgaa gcccggcgac cgcgtggcca tggtggcgcc ggcgtcgccg ccctcaggcc
     1741 ggaggctgct cctggccggg aagaagcggc tcgaacagct cgggctcgag gtggtggccg
     1801 gccggcacgt gatgtccgag tacggctatc ttgccggccc ggaccgcgag cggcggcagg
     1861 acctcgaatc ggctttccgc gaccctcgca tcaaggccat cttctgcgcg cgcggcggct
     1921 acggcgtgtc gcggctgctc tcggggttcg acgccggcct cgcgcgccgc tatcccaagg
     1981 cactcgtcgg cttctccgac atcacggtgc tgcacctcgc gctgcagaag gccggggtgg
     2041 tgtcgttctg ggggccgatg ccctgcacgg ggctcgggtg gacgcccttc tcggttcgcg
     2101 ggctgcagcg ggtgctgatg tcggccgggc cggcggggcg gctgcctttc tcggcgcacc
     2161 gccggccaag gacgctgcgg cgcggggtgg gggaggggcc gctcaccgga ggcaccctga
     2221 gcctgatggc cgccagcctc ggcacgccct acgagatcga gacgcgcggc cgcatcgtct
     2281 tcatggagga cgtggacgag gagccctacc gggtggaccg cctgctcgcg cagttgattg
     2341 ccgcggggaa gctcgccgac gccgccggcc tcgcgctcgg catcttcacg gggaccgacg
     2401 tccgcaactc gccggggaag cgcagcctga ccatgacgca ggtgttcgcc gatcacctgc
     2461 tgccgctgaa ggtgccggtg ctggtgaacc tcgcggtggg gcacgtcccc gaccaggtga
     2521 cgctcccgta cggcgccagg gcgcgcctgg atgcaggggc gaagacgctg gaactgcttg
     2581 agcccggagt cggagattga aaaaggctca gggccagtgg cacaggcgtc ctcgcctgtg
     2641 agcagcctga actacgacca tgaaaactcg ttgccagatt gctctatcca tcgcgctggt
     2701 cttgctggcc agccgtgcgt ttggcgcgcc ggcggtcggg ttccgagccg cgaagccggt
     2761 gtggcccgaa ggacgcgcca ccgagatgaa cctgctcgtc gggttccgcg cggtgatccc
     2821 cgcgccgtct gccggagaaa aggtcgtgct gcgcgtcgcg gcctcgacgc tgtaccgcgc
     2881 gtcggtcaac ggcacgttcg tcggccacgg gcctgcgcgt gcggcccacg ggttctaccg
     2941 ggtggacgag tgggacgtca cggccgcgct cggccgcggg cctgccgtcg tgagcctcga
     3001 ggtggcaggc tacaacgcca acagttacta cctgctcgac cagccgtcct ttctgctggc
     3061 agaggtcacg gccggcgata cggttcttgc ggccacgagg gcaggggcga cgaagagcgg
     3121 aagtcggaag acggggaatg cggagcggcc ggttccgttc gacgctgcga tcctcgacca
     3181 ccgcgtccag aaggtacagc gttacagctt ccagcggccg ttcatcgagg tctaccgcct
     3241 ggcgcccggc ttcgaccggt ggctgcgcga gccggccgcg ccgttccggg tgatcgaggt
     3301 ggaagaggtt tcgccgaaga cgctgctgcc gcgccgcgtg ccctaccccg acttcgccgt
     3361 cacgcgcccc gtggcacacg tggccgaggg gcgcgtggag aagagggcgc aggtcgagcg
     3421 cctctggaag gaccgggcgc tcaccaacat cggcccgaag ctcaaggggt tcccgcaagg
     3481 cgagctggaa cgggtggtgt ccaccgagat gcagcacctc gggtccacgc agccggcctc
     3541 actcaaccgg aagtggcggg agggcgacga ggccgcgctc gccgcggggc actaccgcat
     3601 cctcgacctg gggcagaacc tgaccggctt catcggcatg acggtcgaag tcgccgcgcg
     3661 aagcgagctg cgcctggcat tcgacgaggt cctgacgggc ggcgacgtcg acttcaagcg
     3721 gctcggcacg gtgagcgtgg tgagctactc gctcgagccc ggcacctacc gcctcgagac
     3781 gctggagccg tacacccttc gctacctgaa ggcgatcaac ctcgaggggg agtgccggct
     3841 ccgcgatgtc tacctgcgcg agctgtcgca cccggccgtt gccggcgccc ggttcgcggc
     3901 cagcgacgag cggctgaacc gcctgttcga ggccggcgtc tcgaccttcc ggcagaacac
     3961 gctcgacgtc ttcatggact gcccgtcgcg cgagcgcgcc ggctggctgt gcgacagctt
     4021 cttcaccgcg cggtcggcct tctcgctgac ggggctgccg gcggtcgagg acgccttctt
     4081 cgagaactac ctgcgggccg cgcgcttcga gcacctgccc gacggcatgc tgccgatgtg
     4141 ctacccggcc gaccactacg acggcgtctt catccccaac tgggcgatgt ggttcgtcgt
     4201 gcagctggag gagtacgtct cgcgcggcgg cgaccgcgag gtggtggagg ggcttcgggc
     4261 gcgcgtgctg cgcctgctcg acttcctcgc gacctacgag aacgccgacg ggctgctcga
     4321 gaagcttccg agctgggtgt tcgtcgagtg gtccgaggcg aacaagttcg tgcaggacgt
     4381 gaactacccg agcaacatgc tctacgcgca ggcgctcgac gtggcgggcc gcctctacgg
     4441 gatgccaggg ctttcggcca aggcggaacg ggtgcgggca accgtcaggc ggcagtcgtt
     4501 cgacggcgag ttcttcgtgg acaacgcggt gcggcgcccc gacggtaccc tgcaggtgac
     4561 gcgcaaccgc accgaggtct gccagtactt cgccttcttc ttcggcgtcg ccgacacgac
     4621 aggtgacgcc gcgctgtggg agaagcttcg cggccgcttc gggcccgatc ggaaggagac
     4681 cagggcgttc cccgagatcc accccgcgaa ctccttcatc ggcaacatgc tgcgcatgga
     4741 gctgctgtca cgcgccgggc gcagccagca gatcctcgag gaatcgatcg gctacctgct
     4801 ctacatggtg gagcgcaccg gcacgctctg ggagaacgtc ggcgccgagg cctcgctcaa
     4861 ccacggcttc gcctcgcaca tcgtccgcac gctgcagcgc gacgtgcttg gcgtgcacgc
     4921 ggtcgatccg ctgcgcaagg tgatcaccct gcggttcggc gacgccaagc tggcatggtg
     4981 cgagggcacc gtgcccacgc cggacgggcc cgtgcgcctg cgctggtggc gcgacggggg
     5041 cgccctcgac taccgggtcg aggtgccggc ggggtacagg gtggaggtcg ggggcaggga
     5101 ccccttcatc ggctcctgct ctaccggcgc ggcaggctga cgttcagcgg cgccatctcg
     5161 ccctcccgca cctggacccg ggtcctgaac gtctcgtaac cgtcgaggcg cacctcgatc
     5221 tcgtgcgggc cctcagcaag cag
//
LOCUS       DAOPQX010000255         5238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17242_length_5238_cov_4.628401, whole genome shotgun sequence.
ACCESSION   DAOPQX010000255 DAOPQX010000000
VERSION     DAOPQX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17242_length_5238_cov_4.628401"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..2264)
                     /gene="buk"
                     /locus_tag="PKK95_12600"
     CDS             complement(36..2264)
                     /gene="buk"
                     /locus_tag="PKK95_12600"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOC19106.1"
                     /translation="MFNIEASFDAQLLAAQGNRPVVVLTEPLDGRILEAVCFLTRYIK
                     PVLLASEADVRAAAARHLGHLDPSRVEYSLSECAFVDIAERPDLVGEFAADCLAHHPH
                     GCEAHDPAAARLAVSRPGIFGIYAVRQGHADVVVGGAVHSPRTFFRPMLSLLANRPVV
                     CQAGLFVLPDDFPLGHFPHNIVVFGDVGVNAVMTPEILAEVAVGTCAVARDLIPEEVL
                     PEIQGALVSYSHKGSDEGPSPETVRRALELVPALLERRIEAGSRYRSIRIIGEVKFSA
                     AISRRSAELYGAQGLGGATNVIICPNLETGNLLYHLYAARFPEAKKFPVIFGMAFRGV
                     DLAMDSTPEDIRLAIKASVLRLQRDGRWTRTPRDTFFRRYRVLVLNPGSTSTKIAVYE
                     GDQEVRSAEIKHPPDALAPFAGRRIAEQHEMRKEAVLGVLAEWGLKVEAFDAVCGRGG
                     LLRPIPHGTYRVGEAILEDLRAGTNGDHASNLGALIAHELASPAGKPAFIVDPVVVDE
                     VAERVKVTGLKEIRRRVISHALNQVASARRFAQESETFYEKVNVIVCHLGGGISIGAH
                     RQGRYIDVNNALDGEGPFSAERSGSLPQGQFAELCFSGKHTHAEIKRLIKGAGGLVSL
                     LGTSDLREVERRVAEGDEEASLVFDALAYQVAKTAAGLLPAFEGERLDRVILTGGMAR
                     STALVERLTRYLSGLPCGITVYPGENELVALAKGALRVLYGKEEAREYEPSPEEQGVG
                     SN"
     gene            complement(2297..2836)
                     /locus_tag="PKK95_12605"
     CDS             complement(2297..2836)
                     /locus_tag="PKK95_12605"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOC19107.1"
                     /translation="MHPWHDCYLDEHSVQRSFPVVIEVPKGSKNKYELDKETGLLRLD
                     RVLFSAVHYPANYGFIPRSYCDDGDPLDALVLGQEPVHPLTLVDARAVGVMRMRDEKG
                     IDDKIVAVSIHDPAFADYTDKAQLPNHVLREMRRFFEDYKRLEQKEVVVEDLLGPADA
                     LRIVHDALELYRRLRRGEL"
     gene            complement(2839..3234)
                     /locus_tag="PKK95_12610"
     CDS             complement(2839..3234)
                     /locus_tag="PKK95_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC19108.1"
                     /translation="MPEGTVLLVDDDRDTLALYGLILETFGFRVVPAENGRTALEAAA
                     GDPPDIVVTDLAMPVMDGVELCVRLRSDPSTRAIPILAVSGQAWNGTDDQARRAGCDE
                     VLLKPCLPDRLVDAVGRLLGRSGRLAVRG"
     gene            3597..3968
                     /locus_tag="PKK95_12615"
     CDS             3597..3968
                     /locus_tag="PKK95_12615"
                     /inference="COORDINATES: protein motif:HMM:NF023184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOC19109.1"
                     /translation="MKLSFPLRGTIDREKRTVRAMVEIYCAGHHGGRRGRPCDACREL
                     LDYSHDRLDRCPYGDEKPSCKSCPVHCYRPDPRERMREVMRYAGPRMLWRHPWLALAH
                     LWQERSRGTPGRPRRRRGGSG"
     gene            3993..>5238
                     /locus_tag="PKK95_12620"
     CDS             3993..>5238
                     /locus_tag="PKK95_12620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOC19110.1"
                     /translation="MKTAIAAALMVPALSVPQPAAGNGPAGGPLHDRIDRAASEVMSK
                     VIGWRRDLHRHPELGNREVRTAGIVAEHLRQLGLEVRTGIARTGVVGVLRGGKPGKVV
                     ALRADMDALPVTEETGLPFASTERAEYDGREVGVMHACGHDAHTSMLMGAAEVLAGLR
                     DELPGTVVFIFQPAEEGAPSGEDGGAAVMIAEGVLDDPKVDAIFGLHVFPFEAGAIQV
                     RPGGFMAGGDTLDIRVKGRQTHGALPWQGVDPIVVASQVVLGLQTIISRQSDLTSAPA
                     VITLGIFQAGNRSNIIPDEARLVGTIRTFDQEMRREIHERVKRTASGIAEAAGTTAEV
                     TIMPGNPITYNDPTLTAQIAPSLARVTTSFLPSPPVTTTSEDFSEYQQRVHGVFFFLG
                     VAPKGLEPAKVEPNHSPRFMVEE"
BASE COUNT          791 a         1840 c         1807 g          800 t
ORIGIN      
        1 ggaagtaatc agaagtcgga aactcggcac gacttctaat tactcccgac cccttgttcc
       61 tccggcgacg gttcgtactc ccgcgcctct tccttcccgt agagcacgcg cagcgccccc
      121 ttggccaggg ctaccagttc gttctcgccg ggatagaccg tgatcccgca cggcaggccc
      181 gagaggtagc gcgtgagccg ctcgacgagc gcggtcgatc gggccatccc gcccgtcagg
      241 atgacccggt cgagacgttc gccttcgaag gccggcagca gccccgcggc cgtcttcgcc
      301 acctggtagg ccagcgcgtc gaaaacgagc gaggcctcct cgtccccctc ggcgacccgg
      361 cgctcgacct cccgcaggtc cgaggtaccg agcaggctga ccagcccgcc ggctcccttg
      421 atgagccgct tgatctccgc gtgggtgtgc ttcccggaga aacacagctc ggcgaactgt
      481 ccttgcggca ggctgcccga tcgctcggcg ctgaaggggc cctcgccgtc gagggcgttg
      541 ttgacgtcga tgtagcgtcc ctgccggtgc gccccgatcg agatgccgcc cccgaggtga
      601 cacacgatca cgttgacctt ctcgtagaag gtctcgctct cctgcgcgaa gcggcgcgcc
      661 gaggccacct ggttcaacgc gtggctgatg acgcggcgcc ggatctcttt cagcccggtg
      721 accttcacgc gctcggccac ctcgtccacc acgacggggt ccacgatgaa ggccggcttg
      781 cccgcggggg aggccagctc gtgggcgatg agtgctccga ggttcgaggc gtggtcgccg
      841 ttagtccctg cgcgcaggtc ctcgaggatc gcctccccca cccggtaggt gccgtggggg
      901 atgggacgca gcagcccgcc ccggccgcac accgcgtcga aggcctcgac cttcagcccc
      961 cactcggcga gcaccccgag caccgcctcc ttccgcatct cgtgctgctc ggcgatgcga
     1021 cggcccgcga agggcgccag cgcgtccggg gggtgcttga tttcggccga ccgcacctcc
     1081 tgatccccct cgtacaccgc gatcttggtc gaggtggacc cggggttcag gacgagcacc
     1141 cggtagcggc ggaagaaagt gtcgcgcggc gtccgcgtcc accgcccgtc gcgctgcagg
     1201 cgcagcaccg acgccttgat ggcgagcctg atgtcctcgg gcgtcgagtc catggcgagg
     1261 tccacgccgc ggaacgccat cccgaagatc accgggaact tcttcgcctc cgggaagcgg
     1321 gccgcgtaga ggtgatagag gaggttcccc gtctcgaggt tcgggcagat gatcacgttg
     1381 gtcgcgccgc ccagtccttg cgcgccgtac agctccgcgg atcgtctcga gatcgcggcg
     1441 ctgaacttca cctcgccgat gatgcggatg ctgcgatacc ggctgccggc ctcgatgcgg
     1501 cgctcgagca gcgccggcac cagctcgagc gcccggcgca cggtctcggg cgacggccct
     1561 tcgtcggacc ccttgtgcga gtacgacacg agcgcaccct ggatctcggg cagcacctcc
     1621 tcggggatga ggtcgcgcgc caccgcgcag gtgcccacgg cgacctccgc gaggatctcg
     1681 ggcgtcatca ccgcgttcac gcccacgtcg ccgaacacga cgatgttgtg cgggaagtgg
     1741 cccagtggga agtcgtcggg cagcacgaag aggcctgcct ggcagacgac cggccggttg
     1801 gccagcaggc tcagcatcgg ccggaagaaa gtgcgcgggc tgtggaccgc cccgcccacc
     1861 accacgtcgg cgtgcccctg cctcaccgcg tagatgccga agatgcccgg ccgcgacacc
     1921 gccagccgcg ctgcggccgg gtcgtgcgcc tcgcagccgt gcgggtggtg cgcgaggcag
     1981 tcggcggcga actcgccgac caggtcggga cgctcggcaa tgtccacgaa cgcgcactcg
     2041 ctcaggctgt actccacccg cgaagggtcg aggtgaccca ggtggcgcgc cgccgccgct
     2101 cgaacgtcgg cctcgctggc cagcagcacc ggcttgatgt agcgcgtcag gaagcagacg
     2161 gcctcgagga tccggccgtc gaggggctcg gtgagcacca cgaccgggcg gttcccctgc
     2221 gccgcgagca actgcgcgtc gaacgaggct tcgatgttga acatgctcgg atcgtggggg
     2281 ggcgatggag atggtactac agctccccgc ggcggagccg gcgatacagt tcgagcgcgt
     2341 cgtggacgat cctgagcgcg tcggccgggc cgagcaggtc ctcgacgacc acctccttct
     2401 gctcgagccg cttgtagtct tcgaagaagc gccgcatctc gcgcagcacg tggttgggca
     2461 gctgcgcctt gtcggtgtag tcggcgaagg ccgggtcgtg gatgctgacc gcgacgatct
     2521 tgtcgtcgat ccccttctcg tcgcgcatcc gcatcacgcc caccgcccgg gcgtccacca
     2581 gcgtgagcgg gtgcaccggc tcctgcccca gcacgagcgc gtcgagcggg tcgccatcgt
     2641 cgcagtagct gcgggggatg aagccgtagt tggccgggta gtgcacggcg ctgaagagca
     2701 cgcggtcgag ccgcaggagc cccgtctcct tgtcgagctc gtacttgttc ttgctgccct
     2761 tgggtacctc gatcacgacc gggaacgagc gctgcaccga gtgctcgtcc aggtagcagt
     2821 cgtgccaggg gtgcatggtc agccgcgcac ggcaaggcgc ccgctgcggc cgagcaggcg
     2881 cccgacggca tcgacgagcc gatcgggcag gcagggtttg agcagcactt cgtcgcagcc
     2941 cgcgcggcgc gcctggtcgt cggtgccgtt ccaggcctgg ccgctcaccg cgaggatcgg
     3001 gatcgcgcgg gtggacgggt cggagcgcag ccgcacgcac agctcgaccc catccatgac
     3061 gggcatcgcc agatcggtga ccacgatgtc gggtggatcc ccggcggccg cctcgagcgc
     3121 ggtacgcccg ttctcggcag gcacgacccg gaacccgaac gtctcgagga tcaagccgta
     3181 gagcgcgagc gtgtcgcggt cgtcgtcgac gagcaggacc gtaccttcag gcatgccccc
     3241 atggctccgg gacgcgcggc aatcgaatgg aaacgaagcc cgcattgtag ctcgtgaagc
     3301 gcgcggcgcg caggtcaaat cacgaaatta gcatcatgca cgattgagtc cggaccttta
     3361 cagtctgcga tctccgtcgc gcccgtcgcc gggtccgggc cgtcccactt aatctcgatc
     3421 ggctatgatg ccgtgaggaa ccgccgtccg gacgggccgg cccggcgcgc tgctccgctc
     3481 gtgacgcggc gcgcccttgt gctgttcagg aacatgacga gccggcgctt ccctgtcgcc
     3541 tttgcgttcg gcctgatggc cacggccgag acgcgccaat cccggcgagc gcagccttga
     3601 agctctcttt tccactgcgc ggcacgatcg atcgcgagaa gcgcaccgtg cgcgcgatgg
     3661 tcgagatcta ctgtgccggc catcacggcg ggcgccgggg gcgtccgtgc gacgcttgcc
     3721 gcgagctgct cgactactcg cacgatcgcc tcgaccgctg tccgtacggc gacgagaagc
     3781 cctcctgcaa gtcctgcccg gtccactgct acaggcctga tccccgcgag cggatgcgcg
     3841 aggtgatgcg ctatgcgggc ccccgaatgc tgtggcgcca cccgtggctg gcgctcgcgc
     3901 acttgtggca ggagcggtcc cgtggaacgc cagggcgacc gcgccggcgc cgcggcggat
     3961 caggttagac tgtgaacctg gaggatctcc ccgtgaagac cgccatcgcc gccgcgctca
     4021 tggttcccgc cctctccgtt ccgcagcctg ccgccggcaa cggcccggcg ggggggccgc
     4081 tgcacgaccg gatcgaccgc gccgcttccg aggtgatgtc gaaggtcatc gggtggcgcc
     4141 gcgacctgca tcggcacccg gaactgggca accgcgaggt gcggaccgcg ggcatcgtgg
     4201 ccgagcacct gcggcagctc ggcctggagg tgcgaaccgg catcgcccgc accggcgtcg
     4261 tgggcgtgct gcgcgggggg aagccgggga aggtcgtcgc gctgcgagcc gacatggacg
     4321 ccctgccggt gaccgaggag accggcctgc cgttcgcctc gacggaacgg gccgagtacg
     4381 acgggcgcga ggtcggggtg atgcacgcct gcggccacga cgcgcacacc tcgatgctga
     4441 tgggtgcggc cgaggtgctg gcaggccttc gggacgagct gccgggtacc gtggtcttca
     4501 tcttccagcc ggcagaggaa ggggcgccct ccggtgagga cggtggcgcg gcggtcatga
     4561 ttgccgaggg cgtgctcgac gatccgaagg tggacgccat cttcgggctt cacgtctttc
     4621 cgttcgaggc gggcgccatc caggtgcggc cgggcgggtt catggcgggc ggcgacacgc
     4681 tcgacatccg cgtcaaggga cgccagacgc acggcgcgct cccctggcag ggcgtcgatc
     4741 cgatcgtcgt ggcctcgcag gtcgttctcg ggctgcagac gatcatcagc cgccagtccg
     4801 acctcacctc ggcgccagcc gtcattacgc tcgggatctt ccaggccggc aaccgcagca
     4861 acatcatccc ggacgaagcg cggctcgtgg ggaccattcg caccttcgac caggagatgc
     4921 gacgcgagat ccacgagcgg gtgaagcgaa ccgcgtcggg catcgcggag gcggccggga
     4981 cgaccgccga ggtcaccatc atgccgggca acccgatcac ctacaacgat cccacgctga
     5041 ccgcgcagat cgcgccgagc ctggcacgcg tgacaaccag cttcctcccg agcccgccgg
     5101 tcacgacgac ctccgaggac ttctcggagt accagcagcg cgtgcacggc gtgttcttct
     5161 tcctcggcgt ggcgccgaag ggcctcgaac ccgcgaaggt cgagccgaac cactctccgc
     5221 ggttcatggt cgaggaag
//
LOCUS       DAOPQX010000256         5219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17322_length_5219_cov_4.677188, whole genome shotgun sequence.
ACCESSION   DAOPQX010000256 DAOPQX010000000
VERSION     DAOPQX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17322_length_5219_cov_4.677188"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3384
                     /locus_tag="PKK95_12625"
     CDS             <1..3384
                     /locus_tag="PKK95_12625"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19111.1"
                     /translation="RLAHGEVEGEGAIGKVIAIDQSPIGRTPRSNPATYTGLSDVVRD
                     LFASTEAAVARGFGRGRFSFNVAGGRCEACQGAGVQQVGMHFLGSVDVVCQTCGGRRF
                     NDETLEVRYRGKTIHDVLEMTLEEASSFLAADRRATRILRVLQELGLGYLRLGQPSTT
                     LSGGEAQRVKLAAELSRPARGHTLYILDEPTTGLHAADVAVLIAAINRLVDQGHTVIV
                     IEHHLDLVRSADRVIDLGPGGGAQGGRLVAMGTPEEVAAHPDSATGAALGAYRDVREA
                     LSQADARSILLQHDTLPAGSGRLSAVPDLVVTGVTTHNLKSIDVTIPANTITVVSGVS
                     GSGKSSLAFDTIFSEGRNRFVEGFSAHARRFLEKRGEAGFDAVSGLTPTVAIRQRTAS
                     RNPRSTVGTMTEIADYWRLLFARIGKDTGRPDGRQPPAPLLSSFFSPNSEQGACPACK
                     GLGTRTECDPDRLVTDPSKSVMGGALDGTRTGRFYGDPFGQHAAILRAAGAALGFDVE
                     RPWCELSDEARRVAMHGAGEREFDVEWHYKRGTRTGVHHFRRRWLGLAGYVNEEYERK
                     HADRRGEAMEGLMRRVVCGACGGTRLKPERLAVQVGGRHIAGWMAMSAAECLEALHAI
                     ETDPSAAGLTPRDVLVSRDLRAEIIRRLERLQHAGLGHLTLDRPASTLSAGEAQRVRL
                     AAALGSGLTGMTYVLDEPTAGLHPRDTGRLLDLVRGLRDAGNTVIIVEHDPGVIRAAD
                     HLIDLGPGAGEQGGRIVAAGTPEEVSRVHASPTGRYLRDGSCRRAIEAKGPRRLEPGI
                     RVTGARVHNLQGLDVELPARGLVAVTGVSGSGKSTLVFDVIAPAVAAHLNHDGGAAAL
                     QGCSVELLHRFSRLVCADGAASPGSGSSLVASQAGVFDRLCTLFAATPGARERGLTKR
                     DFSAAVKGGRCEACEGQGRIRISMDFLPDVFVPCEACGGRRYGPEALACRLDGRSIAD
                     VLDLTIAQAREIIQDEHLVRHLAPFYELGLGYLRLGQASATLSGGERQRLRLAAELSG
                     AMGKDEQTHPALFLFDEPTTGLHVDDVARLLEVFDRLVSAGHTIVCVEHNLDVIRAAD
                     WVIDLGPEGGPGGGRLVATGTPAQIAAVESSWTGRALNA"
     gene            3414..3746
                     /locus_tag="PKK95_12630"
     CDS             3414..3746
                     /locus_tag="PKK95_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19112.1"
                     /translation="MAKRTWPGSSSVGKGKRRGHHSVYVVYLRNPKGDRRAGYYVGMT
                     GLTPEQRFANHKAGAKSAGIVRRCGERLVPRLYAHLNPMTYAEALETESRLAKELEAK
                     GYQVFGGH"
     gene            3762..4391
                     /locus_tag="PKK95_12635"
     CDS             3762..4391
                     /locus_tag="PKK95_12635"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOC19113.1"
                     /translation="MNCRNCGAAMDFHESRRYFRCAHCGSIHFPEPAKGDVRVLGVEA
                     TAPPCPVCGVALASALLSERAVHYCERCRGLLLDRGTFVEVVQTKRAWAAAEPREPRP
                     VDPREAEREALCPRCRARMATHPYYGPGRIIIDTCEACDLIWLDPGELDRVVDAPGRD
                     RGSSLRGGEGAWRDREERGVRHDDDDDERRRSGWRSSGRINLLDLLFDG"
     gene            4866..>5219
                     /locus_tag="PKK95_12640"
     CDS             4866..>5219
                     /locus_tag="PKK95_12640"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOC19114.1"
                     /translation="MIAALALAAIVSAPALTSAQVLYGSITGTVSDPQGAALPGVTVT
                     AVNTGTGLKLEQVTDGTGDFTFRNMVPGVYDLSASLEGFKELRQTGVNVTAGNPIRMN
                     LRLELGGITETVNVSA"
BASE COUNT          783 a         1775 c         1833 g          828 t
ORIGIN      
        1 cgtctcgcgc acggcgaggt cgagggcgaa ggggcgatcg ggaaagtgat tgccatcgac
       61 cagtcgccaa tcggccgaac accccgctcg aaccccgcta cctacacggg cctttccgac
      121 gttgtccgcg acctcttcgc ctcgacggag gccgctgtcg cgaggggctt cggtcgggga
      181 cgcttctcgt tcaacgtggc gggcggacgc tgcgaggcct gccagggtgc cggcgtgcag
      241 caggtcggca tgcacttcct cggctccgtg gacgtcgtct gccaaacgtg cggcgggcgg
      301 cggttcaacg acgagacgct ggaggtgagg taccgcggga agacgatcca cgacgtgctc
      361 gagatgacgc tcgaggaagc ttcgtcgttc ctcgcggcag accggcgggc cacacgcatc
      421 ctgcgcgtac tgcaggagct gggactcggc tacctgcgcc tcgggcagcc ctcgacgacc
      481 ttgtcaggag gcgaggcgca gcgggtgaag ctggcggccg agctgagccg gcccgcgcgc
      541 gggcatacgc tctacatcct cgacgagccc accaccggcc tccacgccgc cgacgtcgcc
      601 gtcctcatcg cggcgatcaa ccgcctggtg gaccaggggc acaccgtcat cgtcatcgag
      661 caccacctcg acctcgtgcg gtccgccgac cgcgtgatcg acctcggccc ggggggtgga
      721 gcacagggcg gccgcctggt ggcgatgggc acgccggaag aggtggccgc tcaccccgat
      781 tccgccaccg gcgctgcgct cggtgcgtat cgtgacgtcc gtgaggctct gtcacaggct
      841 gatgcccggt ccatcctcct tcagcacgac acgctgccag ctggcagcgg ccggctgtcc
      901 gctgttcccg atctcgtcgt gaccggtgtc accacccaca acctgaagtc gatcgacgtc
      961 acgattcccg ccaacacgat cacggtcgtg agcggcgtgt cgggcagcgg gaagtcgtcg
     1021 ctcgccttcg acacgatctt ctcggaggga cgcaaccgct tcgtcgaggg gttctcggcc
     1081 cacgcgcggc gcttcctcga gaagcgtggc gaagccgggt tcgacgccgt ctcaggcctc
     1141 acgccaaccg tggcgatccg ccaacgcacg gcctcgcgca atccccgttc gaccgtgggg
     1201 acgatgaccg agattgccga ctattggagg ctgctgttcg cacgcatcgg caaggacacg
     1261 gggcggccag acgggcgaca gccgccagct cctctcttgt cttcgttctt ctccccgaac
     1321 tccgagcaag gtgcctgccc ggcgtgcaag gggctcggga cgcgcaccga gtgcgatccc
     1381 gatcgcctgg tcaccgaccc gtcgaagtcc gttatgggtg gagcgctgga cggcacccgt
     1441 accgggcggt tctacggaga cccgttcggc cagcacgccg ccatcctccg tgcggccggc
     1501 gcggccctcg ggttcgacgt cgagcggccc tggtgcgagc tgtccgacga ggcgcgccga
     1561 gtggccatgc acggggccgg cgagcgcgag ttcgatgtcg agtggcacta caagcgcggc
     1621 acgcgcacag gggtgcatca tttccgccga cgctggctcg gcctggccgg ctacgtcaac
     1681 gaggagtacg agcggaagca cgccgatcgc cggggcgagg cgatggaggg cctgatgcgc
     1741 cgggtcgtgt gcggtgcgtg cggcggcaca cgcctgaagc ccgagcggct cgccgtgcag
     1801 gtgggggggc gtcacatcgc cggctggatg gcaatgagcg ccgccgagtg cctcgaggcg
     1861 ctgcacgcga ttgaaacgga tccctcggcc gcaggcctca cgccgcgaga cgtcctggtc
     1921 agccgcgatc tgcgcgcgga gatcatccgc cgcctcgagc gcctccagca tgcaggcctt
     1981 ggtcatctca cgctcgatcg acctgcgtcc acgctgtcgg ccggcgaggc gcagcgcgtg
     2041 cgcctggccg ccgctcttgg cagcggcctg accggcatga cctacgtgct cgacgagccg
     2101 acggccggct tgcatccccg cgataccggg cgcctgctcg acctggtccg cggcctccgc
     2161 gacgcgggca acacggtcat catcgtcgag cacgaccccg gcgtgattcg ggccgccgac
     2221 cacctgattg atcttggccc cggcgccggc gaacagggtg ggcgcatcgt cgcggctggc
     2281 acacccgagg aggtgtcgcg cgtccacgcc tcgccgaccg ggcgatacct gcgcgacggc
     2341 tcatgtcgaa gggcgatcga ggcgaagggg ccgcggcgtc tcgaacccgg catcagggtc
     2401 accggagcgc gggtgcacaa cctgcaggga ctcgacgtgg aattgccggc ccggggcctc
     2461 gtcgctgtca ccggcgtgtc gggcagcggc aagtccaccc tggttttcga cgtcatcgct
     2521 ccggccgtgg ccgcgcacct gaaccacgac ggaggtgccg ccgcccttca gggctgctca
     2581 gtggaactcc tccatcggtt ctcccggctg gtctgcgcgg acggcgccgc ttctcccggg
     2641 agtggctcga gccttgtcgc cagccaggcc ggcgttttcg atcgcctgtg cacgctcttc
     2701 gcggcgacgc caggggcgcg ggagcgcggc ctgacgaagc gcgacttctc ggcggccgtg
     2761 aagggtgggc gctgcgaggc gtgcgagggg cagggccgca tccgcatcag catggacttc
     2821 ctgcccgacg tcttcgtgcc ttgcgaggcg tgcggcgggc gccgctacgg tcccgaggcg
     2881 cttgcgtgcc ggctcgacgg gcgctcgatc gcggacgtcc tcgacctgac gatcgcccag
     2941 gcgcgcgaga tcatccagga cgaacacctc gtccggcacc tggcgccctt ctacgaactc
     3001 ggccttgggt atctgcgcct gggccaggcc agcgccacgt tgtcgggcgg cgagcgtcaa
     3061 cggctgcgcc tggcggcgga actgtccggc gcgatgggga aggacgagca aactcaccct
     3121 gccttgttcc tgttcgacga gcccactacc ggcctgcacg tggacgatgt ggcgcggctg
     3181 ctggaggtgt tcgaccgcct cgtctccgcc ggtcacacca tcgtctgcgt cgagcacaac
     3241 ctcgacgtga tccgtgcggc cgactgggtc atcgacctcg gtccggaggg cggcccgggc
     3301 ggcggccggc ttgtcgccac cggcacgccc gcccagatcg ccgccgtgga aagctcgtgg
     3361 accggcaggg cgctgaacgc ttgagccttg acggctgctg cacaatccct ggcatggcca
     3421 agcggacgtg gccgggttcc tcgagcgtcg ggaaggggaa gcggcgcggc caccactcgg
     3481 tatacgtcgt ctacctgcgc aatcccaagg gcgacaggcg cgccggctac tatgtcggca
     3541 tgaccggtct cacccccgag caacggttcg cgaaccacaa ggccggtgcg aagtcggccg
     3601 gcatcgtccg gcgctgcggc gagaggctgg ttccacggct ctacgcccac ctcaacccga
     3661 tgacgtacgc cgaggcgctc gagacggagt cgcgactggc gaaggagctc gaggcgaagg
     3721 gttaccaggt gttcggggga cactgaggaa gggccgaagc gatgaactgc aggaactgcg
     3781 gcgccgcgat ggacttccac gaatcgcgcc gctacttccg gtgcgcgcac tgcgggagca
     3841 tccactttcc ggagccggcc aaaggggacg tccgggtgct gggtgtcgag gccacggcgc
     3901 caccgtgtcc cgtgtgcgga gtcgcgctcg cctcggctct tctctcggaa cgcgcggttc
     3961 attattgtga gcggtgccgg gggcttctcc tggaccgcgg tacgttcgtc gaggtcgtcc
     4021 agaccaagcg ggcctgggcg gccgcggaac cgcgcgagcc gcgaccggtc gatccacgcg
     4081 aggccgagcg cgaggcgctg tgcccgcgct gccgcgcgcg gatggcgacc cacccgtact
     4141 acggacctgg ccgcatcatc atcgacacgt gcgaggcgtg cgatctgatc tggctcgacc
     4201 ccggcgagct ggatcgggtc gtggacgccc ccggccgtga ccggggaagc agcctgcgcg
     4261 gcggggaagg ggcctggcga gaccgggaag aacgcggcgt tcggcacgat gacgatgatg
     4321 acgagcgccg gcgctcgggc tggcgttctt ccggccggat caacctgctc gatctgctgt
     4381 tcgacggttg acgcagcagt ccattgtcca cgcctgtgag tcactgtggc ccagtggcac
     4441 aggcgtcttg ccgctcaccg gagcacggcg atccatttca ttggatccca aaaaccatgg
     4501 agatggattc caaggggcct cgccggccgc gtttcccacg gcccgtgttc aggcttgacc
     4561 tcggccggct gaaataggag tattctcagg cgtttggaca ttcctgcctc ggcccggtgg
     4621 ggggcggtgg cagctgggct atggcttgcc tattgcagag ttgatcgagc tggctttcac
     4681 tcgtccgtaa atcgttcgta atcgttccca aacagcacac gtttccggcg gcgttcgggc
     4741 ccatcgtcgt tctttggcgt cgttccgttc ctggcgacag cgaggccgta agcaagcctc
     4801 tttttaacca gcggaaggag ggttcggtca tggccattcg gaacaggcgt ggcagggtgg
     4861 cgctgatgat cgcagcgtta gccctggccg ccatcgtgag cgcaccggcg ctcacgagtg
     4921 cgcaggtcct gtacggctcg ataaccggca cggtgagcga cccgcagggc gccgcgctcc
     4981 caggcgtcac ggtgactgcg gtcaacaccg gcacggggct caagctggag caggtgacag
     5041 acggcaccgg cgatttcacg ttccgcaaca tggtgccggg cgtctacgac ctgtccgcat
     5101 cgctcgaggg cttcaaggag ctgcggcaga cgggcgtgaa cgtgacggcg ggcaacccga
     5161 tccgcatgaa cctgcggctc gagctgggcg ggatcaccga gacggtgaac gtcagcgcc
//
LOCUS       DAOPQX010000257         5183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17480_length_5183_cov_5.258385, whole genome shotgun sequence.
ACCESSION   DAOPQX010000257 DAOPQX010000000
VERSION     DAOPQX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17480_length_5183_cov_5.258385"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKK95_12645"
     CDS             <1..216
                     /locus_tag="PKK95_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19115.1"
                     /translation="DPVSAATAATAVGACSVEAPDATSGVRRWTETAARLSAGWARLP
                     VHAGLAAHSWRRDPRGTFFDPYTQETT"
     gene            213..1187
                     /locus_tag="PKK95_12650"
     CDS             213..1187
                     /locus_tag="PKK95_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19116.1"
                     /translation="MRTLDAKLQAIHADPAGAPDFILADARDADMAFGLASPGVDPAT
                     GRMRSLAEYRDQIREIVRQGLVDVMLMSASTNDVLAREGLFAGSAVTPAVRANDATDI
                     HVLAGAPYPKQPSRPFRTPRIDELMLGGADLALYSITPAGDAERDATALEAYREFRAE
                     AEAAGLRHFLELFAPNVPVPMPDIGRFLNDFVARTLAGIPARGRPIFLKMPYSGPQAM
                     EELVAYDPHLVPGVLGGSAGTTHDAFRLLEEARRHGARVALFGRKIKNAEHQLSFVRW
                     LRAIADGEIGAAEACRAYHGDLERLGIRPHRSLEADLQLTEAVLGGRE"
     gene            complement(1281..2552)
                     /gene="larA"
                     /locus_tag="PKK95_12655"
     CDS             complement(1281..2552)
                     /gene="larA"
                     /locus_tag="PKK95_12655"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOC19117.1"
                     /translation="MMVSLRYGRGELRVEVPSTRASVVEPRFVPGLPDEAAAFREAVR
                     HPIGARPLAECVGAGERLAVAIPDLTRPLPSHRLLPWVFDELSHVPADRVTIVNGTGS
                     HRVNTEEELRSMVGAGVLERYRVVNHSAHEPAGLALAGHTPDGHPVHMNKEWVQADRR
                     VVLGFIEPHFMAGFSGGYKGVFPALADIDAIMHYHRAAVIGDPRSTWGRLEDNPTQEQ
                     VRANGALCPVDFCVNVTLNRDGAITGFYCGDPIEAHREGCRFAKATAMAPQATPFPIV
                     VTTNAGYPLDQNLYQAVKGMSAAAQIVEPGGFILTAARCNDGFPEHGNFRRLMTGSDG
                     PQAILDMINAPGFSVFDQWEAQLLAMIQVKARVALFSELPPDEVRAAHLEPVADIGER
                     LCEELARRGNVPVAILPEGPMTIPYLTRGRE"
     gene            complement(2549..4735)
                     /locus_tag="PKK95_12660"
     CDS             complement(2549..4735)
                     /locus_tag="PKK95_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOC19118.1"
                     /translation="MRTTLLSVCLLAAIAGLSASPQGGYPFQDPNLAAEQRIADLIGR
                     MTLDEKIDCLANRAAVPRLGVKGSPHIEGYHGVAQGGPSNWGRRNPAATTQFPQAYGL
                     GSTWDAELVRRVAAQEALEARYLYQSPRYARSGIIVRAPNADLARDPRWGRTEEVYGE
                     DPFHVGTLATAFTRGLQGDDPRYWTTASLLKHFLANSNEDLRTSSSSDFDERLWREYY
                     AKPFEMAVREGGSRALMAAYNAVNGTPAHVHPMLRDIVMKEWGVDGIICTDGSGLRLL
                     VTDHKAFPDLATAAAASIKAGINHFLDQHKEPVTEAVKRGLLTEAEIDEALRGLFRVS
                     IRLGLLDPPERVPAASVGAPDDPEPWAQPETHALVREVTRKSIVLLKNSAGLLPLDRS
                     KVRSLAIVGPLVNQVLLDWYSGTPPYTVTPRDGIERAAGSPQPGGSRVGTNWVGDMSD
                     GAVELARRQDAAVVCVGSHPEGNAGWALVSSTSEGKEAVDRKAITLPPGQEEFIRRVY
                     EANPRTVVVLITSFPVALPWAAENASTILHVTHNSQELGNALGDVLFGEADPGGRLVQ
                     TWPRSLEQLPPMMDYDIRRGRTYMYFRGEPQYPFGYGLSYTTFAWERLRTSAASMGRD
                     GAIDVSVDVRNTGTREGDEVVQLYVRYPGSKVERPLKQLRGFARATLRPGETRTVTLR
                     LPASDLAYWDPARHAWVVEPGQVELMVGRSSADADLTLKQMVTVTP"
     gene            complement(4791..>5183)
                     /locus_tag="PKK95_12665"
     CDS             complement(4791..>5183)
                     /locus_tag="PKK95_12665"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC19119.1"
                     /translation="GGGLVSTAADYLRFATMLAHGGELDGIRVLGPRTVALMSSNQLP
                     PGVSLPSWGGKNDGNGYGFTMSVTTDVARSTGYGSVGDFGWDGAASTFFRVDPREELV
                     VLLMTQRMPCDLEIQVKLKSLVYQALVD"
BASE COUNT          748 a         1911 c         1763 g          761 t
ORIGIN      
        1 gaccctgtgt ccgccgccac cgccgccacc gccgtcggcg cctgcagcgt ggaagcgccc
       61 gacgccacga gcggcgtgcg ccgctggacg gaaaccgcgg cgcgcctctc ggccggctgg
      121 gcgcggctgc ccgttcacgc cggcctggcc gcccactcct ggcggcgcga cccgcgcggc
      181 accttcttcg acccgtacac ccaggagacg acatgaggac gctcgacgca aagctgcagg
      241 cgatccacgc cgacccggca ggggcacccg atttcatcct cgcagacgcc agggacgccg
      301 acatggcgtt cggactggcc tcgccagggg tggacccggc aaccgggagg atgcgatcgc
      361 tcgcggagta ccgcgaccag atccgcgaga tcgtccgcca ggggctcgtg gacgtcatgc
      421 tgatgagcgc gagcacgaac gacgtcctgg ctcgcgaggg gctcttcgcc gggtcggccg
      481 tgacgcccgc cgtgcgcgcc aacgacgcca ccgacatcca cgtgctggcc ggcgcccctt
      541 acccgaagca gccctcgcgg ccattccgga ccccccgcat cgacgagctg atgctcggcg
      601 gagccgacct ggcgctctac tcgatcacgc cggcaggcga cgccgagcgc gacgccaccg
      661 cgctcgaggc gtaccgcgag ttccgcgccg aggccgaggc ggccggcctt cggcacttcc
      721 tcgagctgtt cgccccgaac gtgccggtgc cgatgcccga catcggccgg ttcctcaacg
      781 acttcgtggc gcgcaccctg gccggcatcc ccgcgcgcgg ccggccgatc ttcctcaaga
      841 tgccgtacag cgggccgcag gcgatggagg agctggtggc ctacgacccg cacctcgtgc
      901 cgggggtcct cggcggctcg gccggcacga cccacgacgc cttccggctt ctcgaggaag
      961 cgcgccgcca cggcgcgcgc gtcgccctgt tcggcaggaa gatcaagaac gccgagcacc
     1021 agctgtcgtt cgtgaggtgg ctgcgggcga ttgccgacgg cgagatcggc gcggccgagg
     1081 cctgccgggc gtaccacggg gacctcgagc ggctcggcat ccggcctcac cgctcgctcg
     1141 aggccgacct gcagctgacc gaggcagtac ttgggggtcg ggagtaatta gaagtcgtgc
     1201 tggaaacccg gcacgactcc taacgggcac gggggggagg agtagtcaga gtcgtgccgg
     1261 aaacccggca cggcttctaa ttactcccga cccctggtca ggtacggaat cgtcatcggc
     1321 ccctcgggca ggatggcgac cggcacgttc ccccgccgcg ccagttcctc gcacagccgc
     1381 tcgccgatgt cggcaacagg ctcgaggtgc gcggcgcgca cctcgtccgg gggtagctcg
     1441 ctgaacagcg ccacgcgcgc cttcacctgg atcatcgcca gcagctgagc ctcccactga
     1501 tcgaacacgc tgaaccccgg cgcgttgatc atgtcgagga tcgcctgcgg accgtccgac
     1561 ccagtcatca gccgccggaa gttgccgtgc tcggggaacc cgtcgttgca gcgcgcggcc
     1621 gtgaggatga agccgcccgg ctcgacaatc tgcgcggccg ccgacatccc cttcaccgcc
     1681 tggtagaggt tctggtcgag cgggtagccg gcgttggtcg tgaccacgat gggaaacggg
     1741 gttgcctgcg gcgccatcgc cgtggccttg gcgaagcggc acccctcgcg gtgcgcttcg
     1801 atcggatcgc cacagtagaa gccggtaatc gccccgtcgc ggttgagggt gacgttgacg
     1861 cagaagtcca cggggcagag cgccccgttc gcgcgcacct gctcctgcgt cgggttgtcc
     1921 tccaggcgtc cccaggtgct gcgcgggtcg ccaatcaccg cggcccggtg gtagtgcatg
     1981 atcgcgtcga tgtcggcgag cgccgggaac acccccttgt agccgcccga aaagcccgcc
     2041 atgaaatggg gctcgatgaa gcccagcacc acccgccggt cggcctgcac ccactccttg
     2101 ttcatgtgca ccgggtggcc gtccggcgtg tgaccggcca gcgcgaggcc ggccggctcg
     2161 tgggccgagt ggttcaccac ccggtagcgc tccagcacgc cggcgccgac catcgagcgc
     2221 agttcctcct cggtgttcac ccggtgggac cccgtgccgt tgacgatggt cacccggtcg
     2281 gccggcacgt gcgacagctc gtcgaacacc cacggcagca gccggtgcga cggcagcggg
     2341 cgcgtgaggt cgggaatcgc caccgccagc cgctctccgg cgccgacgca ctcggccagc
     2401 gggcgcgccc caatcgggtg gcgcaccgct tcccggaacg ccgccgcctc gtcgggcagg
     2461 cccggcacga acctcggctc gaccacgctc gcgcgcgtcg agggcacctc gacgcgcagc
     2521 tcgccgcgcc cgtatcggag gctcaccatc atggcgtcac cgtcaccatt tgcttgagcg
     2581 tgaggtcggc gtcggccgac gagcggccca ccatcagctc cacctgcccg ggctcgacga
     2641 cccatgcgtg gcgcgcggga tcccagtacg ccaggtccga ggcgggcagg cgaagcgtca
     2701 cggtccgcgt ctcccccggg cgaagcgtgg cccgcgcgaa gccccgcagc tgcttgagcg
     2761 ggcgctcgac cttcgagccg ggatagcgca cgtacagctg cacgacctcg tcgccctcgc
     2821 gtgtccccgt gttcctgacg tccaccgaca cgtcgatggc cccgtcgcgc cccatcgacg
     2881 cggcgctggt gcggaggcgc tcccacgcga aggtcgtgta gctcaggccg tagccgaacg
     2941 ggtactgcgg ctcgccgcgg aagtacatgt aggtgcggcc gcggcggatg tcgtagtcca
     3001 tcatcggcgg gagctgctcg agcgagcgcg gccaggtctg caccagccgt ccgccgggat
     3061 cggcctcgcc gaacagcacg tcgccgagcg cgttgcccag ctcctggctg ttgtgcgtca
     3121 cgtggaggat cgtcgacgcg ttttcggccg cccacggcag cgccacgggg aagctggtga
     3181 tcaggacgac caccgtccgc gggttcgcct cgtagacccg gcggatgaac tcttcctgcc
     3241 cgggcggcag cgtgatcgcc ttgcggtcca ccgcctcctt gccctcgctc gtggacgaga
     3301 ccagcgccca gccggcattc ccctcggggt ggctgcccac gcagacgaca gcggcatcct
     3361 ggcgccgcgc cagttccacg gcgccgtcgc tcatgtcgcc gacccagttg gttcccacgc
     3421 ggctgccccc cggctgcggc gaaccggccg cgcgctcgat cccgtcgcgc ggggtcaccg
     3481 tgtagggcgg tgtgcccgag taccagtcga gcagcacctg gttcaccagc gggccgacga
     3541 tggccagcga tctcaccttg gagcggtcca gcggcagcag gccggccgag ttcttcagga
     3601 ggacgatcga cttgcgcgtc acctcgcgca ccagggcgtg cgtctcgggc tgcgcccacg
     3661 gctcggggtc gtccggcgcc ccgaccgacg cggccggcac ccgctcgggc ggatccagca
     3721 gcccgagccg gatcgacacg cggaacagcc cccgcagcgc ctcgtcgatc tcggcctcgg
     3781 tgagcaggcc tcgcttcacg gcctcggtca ccggctcctt gtgctggtcg aggaagtggt
     3841 tgatgccggc cttgatcgac gccgccgcgg cggtggcgag gtcggggaac gccttgtggt
     3901 cggtcaccag cagccgcagg cccgagccgt ccgtgcagat gatcccgtcc accccccatt
     3961 ccttcatcac gatgtcgcgc agcatggggt ggacgtgcgc gggcgtgccg ttcaccgcgt
     4021 tgtaggccgc catcagcgcc cgcgatcccc cctcgcgcac ggccatctcg aacggcttcg
     4081 cgtagtactc gcgccacagc cgctcgtcga aatcggacga ggaactggtg cgcagatcct
     4141 cgttgctgtt ggccaggaaa tgcttcagca gcgatgccgt cgtccagtag cgcgggtcgt
     4201 cgccctgcag gccgcgcgtg aaggccgtgg ccagggtgcc gacgtggaac ggatcttcgc
     4261 cgtacacctc ctcggtgcga ccccagcgcg ggtcgcgcgc caggtcggcg ttgggggcgc
     4321 ggacgatgat ccccgagcgc gcgtaccgcg ggctctggta gaggtatcgc gcctcgagag
     4381 cctcttgtgc cgccacccgc cggaccagct cggcatccca ggtcgagccc agcccgtagg
     4441 cctgcggaaa ctgcgtcgtc gccgccgggt tgcggcggcc ccagttgctc ggccctccct
     4501 gcgcgactcc gtggtagccc tcgatgtgcg gcgacccctt cacgccaagc cgcgggaccg
     4561 cggcccggtt ggcgaggcag tcgatcttct cgtcgagcgt catccggccg atcaggtcgg
     4621 cgatccgctg ctcggccgcg aggtttggat cctggaacgg gtagcctccc tgcggcgacg
     4681 cggagaggcc ggcgattgca gccagcaggc acaccgacag cagcgtggtt cgcatgtcct
     4741 tctcccaagg caggccgccg aagacaacgg cccgaagtca gagcagcctg ctagtccacc
     4801 agcgcctggt agaccaacga cttcagcttc acctggatct cgaggtcgca cggcatccgc
     4861 tgtgtcatca gcagcacgac cagctcttcc ctcggatcga cgcggaagaa ggtggacgcg
     4921 gcgccgtccc agccgaaatc cccgaccgat ccgtaccccg tcgaccgcgc gacgtcggtt
     4981 gtgaccgaca tggtgaaccc gtatccgttc ccgtcgttct tgcctcccca ggacgggagc
     5041 gagacgccgg gggggagctg gttgctgctc atcagtgcca cggttcgcgg cccgaggacc
     5101 cggataccgt ccagttcgcc gccgtgtgcg agcatcgtcg cgaagcggag gtaatcggcc
     5161 gccgtggata ccagccctcc gcc
//
LOCUS       DAOPQX010000258         5183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17482_length_5183_cov_4.063768, whole genome shotgun sequence.
ACCESSION   DAOPQX010000258 DAOPQX010000000
VERSION     DAOPQX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17482_length_5183_cov_4.063768"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKK95_12670"
     CDS             <1..231
                     /locus_tag="PKK95_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19120.1"
                     /translation="RYRDCRHDTEPGCAVKEAVESGALDADRFEHYRKLEREAQANEL
                     RHDEHRRRQAERVWGQLSDEVAQLRRWKGGKP"
     gene            363..809
                     /locus_tag="PKK95_12675"
     CDS             363..809
                     /locus_tag="PKK95_12675"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOC19121.1"
                     /translation="MITPLSLLSLLPLLPLVASGPPSDVAATIIAMERAALDRSDKLD
                     PDGFLEISDPGVVYIDPSLEQPIVGLENLRKYYRSFGEGGEPTSGEMLNSRVQVTGDA
                     AVLTFNYRRKDGKEPRWNATEVYHKTERGWRIVHTHWSYIKPQPPK"
     gene            806..1261
                     /locus_tag="PKK95_12680"
     CDS             806..1261
                     /locus_tag="PKK95_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOC19122.1"
                     /translation="MKIHENDILEVMDGADAVYLATVSGSAPRIRAMVNHRRRDIYPG
                     PSEFARAGGFTVYFCTSAASGKVRELRANPAVAAYFALPRESRGVMLTGDAEVLEDME
                     SKEALWDERWRVYWPGGPSDPDCIVVRLTPREATGWWGSEPFHLDLRAP"
     gene            1258..1668
                     /locus_tag="PKK95_12685"
     CDS             1258..1668
                     /locus_tag="PKK95_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOC19123.1"
                     /translation="MSEQRQGGFLIAKIHRIGGRIFARMLRDRGLEIDPAHGRVLFLL
                     WQEGSMAINELAKRVSLGRSTLTNTIDRLEAAGQIVRVRSVEDRRKILIELTPKNQAL
                     HRLYEDVSAEMTRLFYRASRTKRPRDSTRTWRAC"
     gene            1807..3090
                     /locus_tag="PKK95_12690"
     CDS             1807..3090
                     /locus_tag="PKK95_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC19124.1"
                     /translation="MDTGLATSSRKGEDAVREAIPVSWAVQARPDPGRWLVVGVLVLS
                     TALSFLDRQVLAALAPLLKQEFNLSNEGYGYVVSAFSLAYAIVALPAGAFVDRVGLTR
                     GLAVVVAVWSLAAMATGLTGGLAGLVACRAWLGAAEAGGIPATGKGFATYLQPGERAV
                     GTALNQVGITIGMVAAPLFAGWLAAAYGWRAAFIATGATGLLWLPLWVFVARRRRGPE
                     PPPAGAPRERGPGRDWRLWALAAANILMMTVYSLWMNWTTVFLVSAHGLTQEQANARL
                     AWIPPVFASVGGLFGGWLSLRLARGGGPLYDARFKASALAAVVLTSTALVPLAPGAGV
                     ATALISLSFFMAVAMSANIYAMPLDLFGPSRAAFAVSVLTASYGLMQTVVAPLFGRLI
                     DRVGFAPVCALSGVLPLASLAVLALVGRHSRDDAL"
     gene            3267..4340
                     /locus_tag="PKK95_12695"
     CDS             3267..4340
                     /locus_tag="PKK95_12695"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC19125.1"
                     /translation="MLLDLPSSAVVPRDLRVIAGEQLFDLVGDSEAFRYMRTRLEQVA
                     ATDATVLLLGETGTGKGIAARALHRLSARRDAPLVHVDCASLPATLIESELFGREKGA
                     FTDARSTQPGRFELAHGGTIFLDEIGDLPLEVQAKLLRIIQEGDFERLGSPRTIHVDV
                     RVMAATNHSLIDDVKAGRFRRDLYYRLNVFPITMPPLRDRRQDIPPLVYHLVRRFAER
                     HRRRIDVVPRPVLDSLVAYDWPGNVRELENVLERAIISSDHSTLQLLEPLTPDTIDDP
                     WHPPGRLADVERSHILRVLHACEWRIEGARGAASVLGLKPSTLRSRMRKLGVKRSLPV
                     RLPGGRLLKGPTTAPESQAAGCR"
     gene            4484..5005
                     /locus_tag="PKK95_12700"
     CDS             4484..5005
                     /locus_tag="PKK95_12700"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HOC19126.1"
                     /translation="MSTLVAVVFNDETTAFEMRTALVKMQKEHLISLEDSVVVIRDHT
                     GRTRLDQAVNLTTAGAWGGGFWGMLIGMLFLNPLLGAALGAGAGALSGRFNDIGLDDK
                     MMKDLAQSFKPGTSALFVLIRKATADKVLDALKDFAGRGKVFKTSLNKDDEQALREAL
                     EKPVPAAARAASS"
BASE COUNT          783 a         1792 c         1784 g          824 t
ORIGIN      
        1 cgctatcggg actgccgcca cgacaccgag cctggctgcg cggtgaagga ggcggtggag
       61 tcgggagcgt tggatgccga tcgcttcgag cactaccgca agctggagcg tgaggcccag
      121 gccaacgagc tgcgccacga cgagcacagg cggcggcagg ccgagcgggt gtgggggcag
      181 ctgtccgacg aggtggcgca gttgcggagg tggaagggag gcaagccgta ggaccgcggc
      241 ggtcggccgc gctcgagggc cagacgcctc cggtcaacgc gccgcccggc cgaccgtcat
      301 ctttcctctt gctattggtt cgatatcgca ctatccttgg tcgaaccatt aaggaggttc
      361 gcatgatcac gccgctttcc ttgctgagcc tgctacccct gcttcccctc gtggcatccg
      421 ggccgccctc cgacgtcgcg gccaccatca tcgccatgga gcgtgccgcc ctggaccggt
      481 ccgacaagtt ggaccccgac ggcttcctgg agatctccga tcccggtgtc gtctacatcg
      541 acccctcact ggaacagccg atcgtggggc tcgagaacct gcgcaagtac tacaggtcgt
      601 ttggcgaagg cggcgagccg acatccggag agatgttgaa ttccagggtg caggtgacgg
      661 gcgacgctgc cgtgctgacc ttcaactacc gccgcaagga cggcaaggag ccgcgctgga
      721 acgccaccga ggtctatcac aagaccgagc gcggctggcg catcgttcac acgcactggt
      781 cgtacatcaa gccgcagccg ccaaaatgaa gatccacgag aacgacatcc tcgaggtgat
      841 ggacggggcc gacgccgtct acctggccac cgtgagcggc tcggccccgc gcattcgcgc
      901 catggtcaac caccggcgcc gggacatcta ccccggtcct tcggagttcg cccgcgcggg
      961 cggattcacc gtctacttct gcacgtcggc tgcctcgggg aaggtgcgcg agctgcgcgc
     1021 caatcccgcc gtcgcggcct acttcgccct gccgcgcgaa tccaggggcg tgatgctcac
     1081 gggcgacgcg gaggttctcg aagacatgga gtccaaggag gcgctctggg acgagcgctg
     1141 gcgggtctac tggcccggcg gaccctccga ccccgattgc atcgtcgtcc gcctcacgcc
     1201 cagggaggcc accgggtggt ggggcagcga accgttccac ctggacctgc gcgcgccatg
     1261 agcgaacaga ggcagggcgg cttcctcatc gcgaagatcc accggatcgg agggcgcatc
     1321 ttcgcgcgca tgctgcgcga ccgcggcctc gagatcgacc ctgcccacgg ccgggtgctc
     1381 ttcttgctgt ggcaggaagg gtcgatggcc atcaacgagc tggccaagcg cgtctcgctc
     1441 ggcaggtcca ccctcacgaa caccatcgac cgcctggaag ccgccgggca gatcgtccgc
     1501 gtgcgcagcg tcgaggatcg gcgcaagatc ctgatcgagc tcacgccaaa gaaccaggcc
     1561 ctgcaccggc tctacgagga tgtctctgcc gagatgacgc gcctcttcta ccgggcttct
     1621 cgaacgaaga ggccgcgcga ttcgacgcgt acctggcgcg cgtgctagcc aatctcgaac
     1681 acggttccat cgcgccggac gttccgcgcc gccgggtgaa agcctcggtt ccccgccgtc
     1741 gccggaagtg attgcgtccg ccgtccgcct gctcttcagg cgcggccggc acgggacgag
     1801 gcgcgaatgg acaccggcct cgcgacctcg agccggaagg gcgaagacgc ggtcagggag
     1861 gcgatcccgg tatcatgggc cgtgcaggca agacccgacc cggggcgctg gctggtcgtc
     1921 ggtgtcctcg tcctttccac cgccctcagc ttcctcgacc gccaggtcct cgccgcgctg
     1981 gcgccgctcc tcaagcagga gttcaacctc tccaacgagg gctacggcta cgtcgtctcg
     2041 gccttctcgc tcgcctacgc gatcgtcgcg ctgccggccg gcgcgttcgt ggaccgcgtc
     2101 ggcctcacgc gcggcctggc ggtggtggtg gcggtctggt cgctggccgc gatggcgacc
     2161 ggtctcaccg ggggcctggc gggcctcgtc gcgtgccgcg cgtggctggg ggcggcggag
     2221 gcgggcggca taccggcgac cggcaagggc ttcgcaacct acctccagcc gggcgagcgg
     2281 gcggtgggga cggccctgaa ccaggtcggg atcaccatcg gcatggtggc ggcgcccctg
     2341 tttgccggct ggctggcggc cgcgtacggg tggcgagcgg cgttcatcgc caccggcgcc
     2401 accggccttc tctggctccc gctctgggtg ttcgtcgccc ggcggcggcg cgggccggag
     2461 ccgccccctg cgggtgcccc gcgcgaacgc gggccggggc gcgactggcg cctgtgggcg
     2521 ctggccgcgg cgaacatcct gatgatgacc gtctacagtc tgtggatgaa ctggacgacg
     2581 gtcttcctgg tgtcggctca cgggctgacg caggagcagg ccaacgcgcg gctggcctgg
     2641 attcccccgg ttttcgcgtc ggtcggcggc ctgttcggcg ggtggctgtc gctgaggctg
     2701 gcgcgcggag gcgggccgct ctacgacgcg cgcttcaagg cgtcggccct ggcggcggtc
     2761 gtcctgacct ccacggcgct cgtgccgctg gcgccgggcg ccggcgtggc gaccgcgctc
     2821 atctcgctga gcttcttcat ggcggtcgcg atgagcgcga acatctacgc catgccgctg
     2881 gatctgttcg gccccagccg cgcggcgttc gccgtctcgg ttctgaccgc gtcgtacggc
     2941 ctgatgcaga ccgtcgtggc ccccttgttc ggccggctca tcgaccgcgt cggcttcgcg
     3001 ccggtgtgtg ccttgtccgg cgtcctcccg ctcgcgtcgc tggcggtgct ggcgctggtc
     3061 ggccgtcatt cgcgcgacga cgctctgtag gacgacggcg tctctgccct atcctctctc
     3121 ctgctctgca gaccgtcacc gcgaagcgac atggtccgat cttggcaggc atcggcgaag
     3181 cggccctgag ccgccgcgct gaaatttctg acggccgcgc tgaccgtgca cgccaaaccg
     3241 cggcccagca ctcgaggacc gatcgtatgc tcctggactt gccttcatcg gcggtcgtcc
     3301 ctcgcgatct gcgagtgatc gccggcgagc agctgttcga cctcgtcggc gacagtgaag
     3361 ccttccgata catgcgcacc aggctggagc aggtggcggc gacggacgcg accgtgctcc
     3421 tgctgggcga aacggggacc ggaaagggga tcgcggcgcg ggcgcttcac cgcttgagtg
     3481 cgcggcgcga cgcgccgctc gtccacgtgg actgcgcgtc gctgcccgcg accctgatcg
     3541 aaagcgagct gttcgggcgg gagaagggcg ccttcaccga cgcgcgctcg acccagcccg
     3601 ggcgcttcga actggcgcac ggcggcacga tctttctcga cgagatcggt gacctgccgc
     3661 tcgaggttca ggcgaagctc ctgcgcatca tccaggaggg tgatttcgag cgcctcgggt
     3721 cgccccgcac gatccacgtg gacgtgcggg tgatggcggc aaccaaccac agcctgatcg
     3781 atgacgtgaa ggccggccgt ttccggcgcg acctgtacta ccgcctgaac gtgttcccca
     3841 tcacgatgcc cccgctccgc gaccgcaggc aggacatccc gccgctggtg taccacctcg
     3901 tcaggcggtt cgcggaacgc caccggcgcc ggatcgacgt cgttccccgc cccgtgctcg
     3961 acagcctcgt cgcctacgac tggcccggga acgtgcgcga gctcgagaac gtgctcgagc
     4021 gagccatcat cagctccgat cactcgacgc ttcagctgct ggagccgctc acgcccgaca
     4081 cgatcgacga cccatggcac ccgcccggaa ggcttgcgga tgtcgagcgt tcgcacatcc
     4141 tccgggtgct gcacgcctgc gaatggcgca tcgagggagc acgcggcgca gcgtcggtcc
     4201 tgggcctgaa gccgagcacg ctccggagcc ggatgcggaa gctcggcgtg aagcgatccc
     4261 ttcccgttcg ccttccgggc ggccgcctgc tcaagggccc gacaacggcg cccgagtcgc
     4321 aagccgccgg atgccgctga cagaccaccg gatgtcgtgg gtgctggtcc gatgttccgc
     4381 ccaagccgcc gtgcggccgg atcgcggcgg tggaacgggt cttggacggg gatggccggg
     4441 cgcgcagaga acctcactca gcactcagcc aaggagcagg cagatgagca cgcttgtggc
     4501 ggttgtcttc aacgacgaga cgacggcttt cgagatgcgg acggcgctcg tcaagatgca
     4561 gaaggagcat ctcatctcgc tcgaagactc ggtcgtcgtg atcagggatc acacggggag
     4621 gacgagactg gaccaggcgg tcaacctgac gacggcaggc gcctggggcg gcggcttctg
     4681 gggcatgctg atcggcatgc tgttcctgaa cccgctgctg ggggcagccc tcggggcggg
     4741 cgccggcgcc ctgtccggac ggttcaacga catcggcctc gacgacaaga tgatgaagga
     4801 cctcgcgcag tccttcaagc cgggcacctc ggcgctcttc gtcctcatcc ggaaggcgac
     4861 ggccgacaag gtgctcgacg cgctgaagga cttcgcgggc cggggcaagg tcttcaagac
     4921 gtcgctcaac aaggacgacg aacaggccct ccgcgaggcg ctcgagaagc ccgttcccgc
     4981 ggcggcccga gcggcttcga gttagtaccc tacgagcccg tggtactcgt gtcccgctga
     5041 gcagcaccga agagggtgac tcccatggcc aacgcgcgcg ccgacgcgat gaacctgacc
     5101 cacacctacc cgcacgagaa gaccgggctc gaacggtttc tggacgtcgt cgaacgggtg
     5161 gggaaccaag ttcctcaccc ggt
//
LOCUS       DAOPQX010000259         5155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17594_length_5155_cov_4.481373, whole genome shotgun sequence.
ACCESSION   DAOPQX010000259 DAOPQX010000000
VERSION     DAOPQX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17594_length_5155_cov_4.481373"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKK95_12705"
     CDS             complement(<1..237)
                     /locus_tag="PKK95_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19127.1"
                     /translation="MFSLRALAPAFIGVAVLAPVAAGGTVVWQGSLEALVRAAETFKT
                     GPSAPKPDYWITIDPEATADGLLGVYDRHAEEFWR"
     gene            481..951
                     /locus_tag="PKK95_12710"
     CDS             481..951
                     /locus_tag="PKK95_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19128.1"
                     /translation="MDFLQAALEHGTYPTLAVVSFVSAIFPVVNAELAMVGLVAALPN
                     PNLLPLVAVATAAQMAGKSMMYWLGRRGGEAVHGRYALAAERWGHRFRGSTWGVGGLV
                     FISSASGLPPFYAISTLAGFFRTSFAAFLLIGSAGRFVHFTALGLFPAALKSLG"
     gene            1032..2393
                     /locus_tag="PKK95_12715"
     CDS             1032..2393
                     /locus_tag="PKK95_12715"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOC19129.1"
                     /translation="MVNWDEARYRGDAQDVDELVRVYRVNDYLATVEANRRQVDRGIR
                     EKLIKHGIRLSERLSPRIYRLFGETCARFGVESEAEVFCIPSAEINAFATLDVREACT
                     YSLIGVTATALERLDDAELRFILGHELGHILFGNNRLDGLLSQNPENADITVLPPFGE
                     SLFLRWRRKAEISADRAGLLAAGSFRDSATALLKATFGLSEKNLNLDVAALVAQVDEI
                     KGHPELMAEAFASHPLVPIRLKAIELFGRSSKAARNGLESSPADLGDEELEDQVDELV
                     RLTRRYPFRPVDRAAMQVVALGGALLLGADAEVSDEEVKILIQILHRFFTDEPETEIV
                     TDPAVVRERLPAALAVVNAEGDATAKNFILSRLTDIALADGALMDAESSVILDLANQL
                     GLPSRVAYGVLVGAAQAIGFRSDVKLNRMSEEIRRGLQSGFARGTVSLPVLLQPDAAP
                     AAI"
     gene            2402..3589
                     /locus_tag="PKK95_12720"
     CDS             2402..3589
                     /locus_tag="PKK95_12720"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOC19130.1"
                     /translation="MNAMDATKTHVIDGTPVVLPGDEAYRVGREDELLTPALLVFPEI
                     VDANIRAIVGLLGGDAGRWRAHVKSAKIASVMRQLVEAGVSNLKCATPLELRVACDAG
                     ASDVLVAYPVVGPAVARVAEIARAHRATRVSALVEAEPAVAAWRGTGVGLFVDVNPGM
                     DRTGIADTAVEAIASVARAIARAGIRFGGLHYYDGQVGTGPGWEARAHAGYDRLIEVV
                     AALEKAGLAVPEVITAGTPAFAASLSYPRFAGRRFLHRVSPGTVVYCDATSAAQLPPE
                     WGLRPAAVVASRVISHPAPGRVTLDAGHKSVSADAGVPTCAIAGVPWLTPLKPSEEHL
                     PVAVPEGRDAPPVGRVLYLVPRHVCPTVNNFDHAAIVREGRIEAVEAVAARGRERPLT
                     AAR"
     gene            complement(3593..4375)
                     /locus_tag="PKK95_12725"
     CDS             complement(3593..4375)
                     /locus_tag="PKK95_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19131.1"
                     /translation="MRPVEQGFTLLAPFLYILEVLTMRVRFVLAATLLAALSVPAAAQ
                     APQPHETASEVPALSAMHEVIMPLWHEAWPAKDTKRMAELTPGIEKYLAEVTGAKLPG
                     ILRDRQGAWDAGLKQLQASVAAYRAAVDKQDDAALLKAAEALHTDYEKLVRVVRPATP
                     EIDAFHQALYVLYHYDLEKFSLPVVKDHVAGLKAKMDALAAAALPARHASRKAAYEPA
                     RDRLAASVNALSTAAAGGDEAKIKAAIEEVHAGYEALDSVFK"
     gene            complement(4405..>5155)
                     /locus_tag="PKK95_12730"
     CDS             complement(4405..>5155)
                     /locus_tag="PKK95_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC19132.1"
                     /translation="EEFFFSAGLLVCQGYGLTETSPMVTCNRPGDFRFGTVGKPIPGC
                     EVKVAADGEILVRGANVTQGYFGRERETRETFDEGWLKTGDIGEIDADGFISITDRKK
                     DLIITSGGKNVAPALVESVIGRDYYIDQVAAVGEGRNYLAALVVPQFEALEAWARERG
                     LTYESLRALVLDARVVAFIAERIEQQQAVLARHEQVKRFALLSERFTQGGGEITPTLK
                     NVRAAIAAKYAAVINAMYSAEWGGGGAAPAG"
BASE COUNT          736 a         1807 c         1821 g          791 t
ORIGIN      
        1 gcgccagaac tcttcggcgt ggcggtcgta aacgcccaac aggccgtcgg cggtcgcctc
       61 ggggtcaatc gtgatccagt agtcgggctt gggcgccgag gggccggtct tgaaggtctc
      121 ggcggcgcga acgagcgcct ccaggctgcc ctgccacacc acggtgccgc ccgcggcaac
      181 cggcgcgagg acggcgacgc cgatgaaggc aggcgccagg gcgcggaggc tgaacatcgc
      241 agggatccgg cactactctt caccctctat cggcgccgtg tcaacaggcg atgaacgata
      301 tctccgcttg cgccggtctt cggcggccgc ctccttctcc ttcgtgtcct ttgtggtttc
      361 cccgtccggg gaggcggcgc ctggcgtcga agttgcactt gggggaggca tccttgccag
      421 ccgggtgacg gcgggccgat ggcggccgcg tggccgtgca aagttcacgg tgtgttgagt
      481 atggatttcc tgcaggcggc gctcgagcac ggcacgtatc cgacgctggc agtcgtgtcg
      541 ttcgtgagcg ccatctttcc cgtagtcaac gcggaactcg cgatggttgg cctggtggcc
      601 gcgctcccga acccgaacct cctgccgctg gttgccgtgg caaccgccgc gcagatggct
      661 ggcaagtcga tgatgtactg gctcggacgc cgcggcggag aggcggtgca cggccggtac
      721 gcgctggcgg cggagcgttg gggccaccgc ttccgcgggt cgacgtgggg agtgggcggg
      781 ctcgtcttca tcagttcggc ctcgggtctg ccgcccttct acgcgatcag tacgttggcg
      841 gggttctttc gcaccagctt tgccgcgttc ctgctgatcg gctcggcggg ccgcttcgtc
      901 cacttcaccg cgctcggcct ctttcccgca gccctcaagt ccctcggttg aaggacgcga
      961 aggcggagcc tgcccgccgt agctcgaggg tccttcccga gagcgaaggc ggggtggtag
     1021 catcccccgc tatggtgaac tgggatgagg cccgctaccg cggcgacgcg caggacgtgg
     1081 acgagctggt tcgcgtttat cgcgtcaacg actacctcgc caccgtcgag gccaaccggc
     1141 gccaggtcga ccgtgggatc cgcgagaagc tgatcaagca cggcatccgg ttgagcgagc
     1201 ggctctcccc ccgcatctac cgcctcttcg gcgagacgtg cgcgcggttc ggcgtcgagt
     1261 cggaggccga ggtgttctgc atcccgtccg ccgagatcaa cgcgttcgcg acgctcgacg
     1321 tccgagaggc ctgtacctac tcgctgatcg gcgtgacggc caccgcgctc gagcggctcg
     1381 acgacgccga gctgcggttc atcctcgggc acgagctggg gcacatcctc ttcggcaaca
     1441 accgcctgga cggcctgttg tcgcagaacc ccgagaacgc cgacatcacg gtgctgccgc
     1501 ccttcggcga gagcctcttc ctgcgctggc ggcgcaaggc cgagatcagc gccgaccgcg
     1561 cggggctgct ggcggccgga agcttccgcg actcggccac ggcgctgctc aaggccactt
     1621 tcggcctgag cgagaagaac ctcaacctcg acgtcgcggc gctggtggcg caggtcgacg
     1681 agatcaaggg acaccccgag ctgatggccg aggcgttcgc ctcgcacccg ctcgtaccaa
     1741 tccgcctgaa ggcaatcgag ctgttcggcc gatcgtcgaa ggccgcgcgc aacggcttgg
     1801 agtcgtcgcc ggcagacctc ggcgacgaag aactggaaga ccaggtggac gagctggtgc
     1861 ggctgacccg gcggtacccg ttccggccgg tggaccgcgc cgccatgcag gtcgtggccc
     1921 tcggcggggc gttgctgctc ggcgccgacg ccgaggtcag cgacgaagaa gtcaagatcc
     1981 tgatccagat cctgcaccgc ttcttcaccg acgagcccga aaccgagatc gtgaccgacc
     2041 cggcggtggt gcgggagcgg ctgccggcgg cgctcgcggt cgtgaacgcg gagggggatg
     2101 cgacggcaaa gaacttcatc ctctcgcggc tcaccgacat cgcgctggcc gacggcgcgc
     2161 tgatggacgc cgagagctcg gtcatcctcg acctggcgaa ccagctggga ctccccagcc
     2221 gcgtggcgta cggcgtgctc gtgggtgctg cacaggcgat cggcttccgc agcgacgtga
     2281 agctgaaccg gatgtccgag gagatccggc gggggctgca gagcggcttc gcgcgcggca
     2341 ccgtctcgct gccggtgctg ctgcagcccg acgccgcgcc ggccgccatc tgagcggggc
     2401 catgaacgca atggacgcca ccaagaccca cgtcatcgac gggacgccgg tggtccttcc
     2461 aggcgacgag gcctaccggg tcgggcggga ggacgaactg ctcaccccgg cactcctggt
     2521 cttccccgag atcgtcgacg ccaacatccg ggcgatcgtc ggcctgctcg ggggcgacgc
     2581 cggacgctgg cgcgcgcacg tgaagagcgc caagatcgcg agcgtcatgc gccagctcgt
     2641 cgaggcaggc gtgtccaacc tgaagtgcgc cacgcctctc gagctgcggg tggcctgcga
     2701 cgccggcgca agcgacgtcc tggtggccta ccccgtcgtc ggacccgcgg tggctcgcgt
     2761 ggccgagatc gctcgcgccc accgggccac gcgcgtgtcg gcgctggtcg aggccgagcc
     2821 ggccgtcgcc gcgtggcgcg ggacgggggt ggggctcttc gtggacgtca accccggcat
     2881 ggaccggacg ggaatcgcgg acaccgccgt ggaggcgatc gcaagcgtgg cgcgggcaat
     2941 cgcgcgcgcg ggaatccgct tcggcgggct ccactactac gacggtcagg tgggaaccgg
     3001 gcccgggtgg gaagcgcgtg cgcacgcggg ctacgaccgc ctgatcgagg tcgtcgcggc
     3061 gctggaaaag gccggcctcg ccgtgcccga ggtgatcacg gccggcacac ctgccttcgc
     3121 ggcatccctg tcgtacccgc gcttcgccgg ccggcgcttc ctccaccgcg tctcgccggg
     3181 gacggtggtg tactgcgatg cgacgagcgc ggcacagctc cccccggagt gggggctccg
     3241 cccggcggcc gtcgtggcca gccgcgtcat cagccaccct gcccccgggc gagtgacgct
     3301 cgatgcaggc cacaagagcg tttcggcgga cgcgggcgtc ccgacctgcg ccatcgccgg
     3361 agtcccgtgg ctgacgcccc tcaagccaag cgaggagcac ctgcccgtcg cggtgccgga
     3421 agggcgcgac gcgccgccgg tcgggcgcgt cctctacctg gtgccgcggc acgtctgccc
     3481 gacggtcaac aacttcgacc acgccgcgat cgtgcgcgag gggcgcatcg aggccgtgga
     3541 agcggtcgcc gccaggggca gggagcggcc gctgaccgcg gcaaggtaga acctatttga
     3601 agacggaatc gagcgcttcg tagcccgcgt ggacctcctc gatcgccgcc ttgatcttcg
     3661 cctcgtcgcc gccagccgcg gcggttgaca gcgcgttcac tgaggccgcc agccggtcgc
     3721 gggcgggctc gtaggcggcc ttcctggagg cgtgccgggc cggcagggcg gccgcggcga
     3781 gcgcgtccat cttcgccttc aaccccgcca cgtgatcctt cacgaccgga agcgaaaact
     3841 tctcgaggtc gtagtgatag agcacgtaga gcgcctggtg gaaggcgtcg atctcggggg
     3901 tcgccggccg gaccacccgc accagcttct cgtagtcggt gtgcagcgcc tcggccgcct
     3961 tgagcagcgc cgcgtcgtcc tgcttgtcca ccgcggcccg gtaggccgca accgacgcct
     4021 gcagctgctt caggccggcg tcccaggccc cctgccggtc gcgcaggatc cccggcagct
     4081 tcgcgccggt cacctcggcc aggtacttct cgatgcccgg cgtcagctcc gccatccgct
     4141 tggtgtcctt cgccggccac gcctcgtgcc agaggggcat gatgacctcg tgcatcgccg
     4201 acagtgccgg cacctcggac gcggtctcgt ggggctgcgg cgcctgtgca gcggccggga
     4261 cggagagggc ggccagcagc gtggccgcga gcacgaatcg cacgcgcatc gtcaggacct
     4321 ccaggatgta gaggaaggga gcgagcaggg tgaagccctg ctccacaggt ctcacgcgtc
     4381 ggcgccgccg gcggtgccgc ggcatcagcc cgcgggcgcc gcgccgccgc cgccccactc
     4441 ggccgagtac atggcgttga tgacggcggc gtacttggcg gcaatggcgg cacggacgtt
     4501 cttcagcgtg ggggtgatct cgccgccgcc ctgcgtgaag cgctcactca ggagcgcgaa
     4561 ccgcttcacc tgctcgtgcc gcgccagcac cgcctgctgc tgctcgatcc gctcggcgat
     4621 gaacgccacg acgcgggcgt cgagcaccag cgccctcagc gactcgtagg tgaggccgcg
     4681 ctcacgggcc caggcttcga gcgcctcgaa ctgcggcacc acgagggccg ccaggtagtt
     4741 gcgcccctcg ccgacggccg cgacctggtc gatgtagtag tccctgccga tcaccgactc
     4801 gaccagggct ggcgcgacgt tcttgccgcc cgaggtgatg atcaggtcct tcttccggtc
     4861 ggtgatgctg atgaagccgt cggcgtcgat ctcgccgatg tcgccggtct tcagccagcc
     4921 ctcgtcgaag gtctcgcgcg tctcgcgctc gcgcccgaag tagccctgtg tcacgttggc
     4981 gccgcggacg aggatctcgc cgtcggcggc caccttcacc tcgcatcccg ggatcggctt
     5041 gccaaccgtg ccgaagcgga agtcgcccgg acggttgcag gtcaccatgg gcgaggtctc
     5101 ggtgaggccg tagccctggc acaccaacag ccccgccgag aagaagaact cctcg
//
LOCUS       DAOPQX010000260         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17661_length_5138_cov_4.271887, whole genome shotgun sequence.
ACCESSION   DAOPQX010000260 DAOPQX010000000
VERSION     DAOPQX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17661_length_5138_cov_4.271887"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1462)
                     /locus_tag="PKK95_12735"
     CDS             complement(59..1462)
                     /locus_tag="PKK95_12735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC19133.1"
                     /translation="MGRILIADDHDALRRGLARALTEAGHEVDEAPNGNAAIERLHES
                     YFDVVLSDLKMGGSDGLDVLRTTKSLHPSTSVILMTAFGSVNTAVEAMKSGAFDYVQK
                     PFEIEEMEVKIEKALELRRMRNELDYLRHTQNDIYEFDRIVGSSGALARVLGVVRKVA
                     KSNTTVLIRGETGTGKELIAGAIHHNSLRASRNFVRVNCAALQENLLESELFGHEKGA
                     FTGADKQRVGRFEQADGGTMFLDEVGDMSPSTQAKILRVLQEHEFERLGGTRTLKVDV
                     RLIAATNRNLASMVATGHFREDLYYRLNVVSIDMPPLRERKDDIAALAEFFIRRFSAE
                     LKKKMEGIHVDALKLLMRYNWPGNIRELENAIERAVLLTEGTQIMAEDLRLGEAVAPV
                     GDAEGVQVVRIPPSGIALEEIERRALVEALKMSNWVQKDAAELLAISPRVINYKIKTL
                     GIELPRARRAAVETEVV"
     gene            complement(1535..2716)
                     /locus_tag="PKK95_12740"
     CDS             complement(1535..2716)
                     /locus_tag="PKK95_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC19134.1"
                     /translation="MTDPQPTPAPPKPRSGAHPATPGQPSLPPVGDRFFRQLVGSMRN
                     GVLAIARDGRVVAMNEVAYRVFNIDPTPDDIGAPYTDVLQDQPDVIRILGAAFELQNL
                     PNRAELKLRTSGKVIGYTLSHVLDDAGDVVGASLFFKDLTLVEQLEERERLRDRLAAL
                     GEMAAAIAHEVKNPLAGIEVMAGLLRRRIPDSPEAQGLINDIIAEAKVANAIVLEVLD
                     FVRPIRLQVERTSIPQVLHDAVTTAERKVSRGGVNVTLNVQEQLPLIQADHHQLVQVF
                     SNLIINALEALNGTGTIAIAAVQGEGYVEPGAEPRRPGPPTVVVDVADSGPGVSPEIL
                     DRVFNPFFTTKPQGSGLGLAIVRKIVDAHNGHIDFTSAAGQGTHFRVTLPMEAGEGWL
                     R"
     gene            complement(2976..>5138)
                     /locus_tag="PKK95_12745"
     CDS             complement(2976..>5138)
                     /locus_tag="PKK95_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOC19135.1"
                     /translation="ACPTGTGPRQARRLSYWDAAQAGRLCYWYNKGFTKAGPFEPMIQ
                     IAGLTKSFGDRVLFDNVTWQISDGERVGLTGPNGSGKTTLLKIFAGLEEPDSGQVVKP
                     ADLTVGYLPQDGIAHQGRTVREEAMLAFGPLLEVRAEMEALEERLGHPDVSDADHEQM
                     LVRYSDLQDRFRLHDGYNMDLKVGTVLQGLGFSHADLDRPTETFSGGWQMRLALGKLL
                     LARPNLLLLDEPTNHLDLEARNWLEEYLHDYPHAVILVSHDRYFLDTVINRVAEVYLR
                     QLNDYPGNYTRYLAESQARLERVREAKKQQDEEIERIKMFISRFRYKATKAAQVQSRV
                     KMLEKVVPIEVPPERKRVHFSFPSSVKSGRTVLELERIRKAYGPTVVFERANLHVERG
                     DRIALVGPNGSGKSTLMRMLAGVERPDSGARREGHQVVMQYFAQDEAVRLDPALTVYE
                     TLQSGSPVHMVPAIRNILGGFLFSGDDVYKKVEVLSGGERTRLAVARMLLRPSNTLLL
                     DEPTNHLDLDSKDVLLEALEDYGGTLIFVSHDRYFVEKLANRVVEIGGGEAHSYPGRY
                     DEFLWSKGQRKGPAPANQPSPPRPGKPGVEAIAAVAPGAAATAATAKSDGRGEPSSAE
                     REARRRQALSEARRRERHQKALRTRVTELEETIAAREAEIKALEAQMASDGFYDNREA
                     VQPVVDRHQALMWEVGDLMHKWEELQHSLERAEQDLQS"
BASE COUNT          708 a         1759 c         1780 g          891 t
ORIGIN      
        1 tggccgtgca gccaggtgaa gcgctgaggt acgagtagcg cagggctaaa gccctgcgct
       61 acacgacctc cgtctctacc gcggcccgcc gcgcgcgcgg cagctcgatg ccgagcgtct
      121 tgatcttgta gttgatgacg cgcgggctga tcgccagcag ctccgcggcg tccttctgca
      181 cccagttcga catcttcagc gcctcgacca gggcgcgccg ctcgatctcc tcgagcgcaa
      241 tccccgaggg cgggatgcgc acgacctgca ccccctcggc gtcgcccacc ggcgccaccg
      301 cctcgccgag gcgcaggtcc tcggccatga tctgcgttcc ctcggtcagc agcaccgccc
      361 gctcgatcgc gttctccagc tcgcggatgt tcccgggcca gttgtagcgc atgagcagct
      421 tgagggcatc gacgtggatc ccctccatct tcttcttcag ctcggccgag aagcgccgga
      481 tgaagaactc ggcgagggcg gcgatgtcgt ccttccgctc gcggagcggc ggcatgtcga
      541 tcgagacgac gttcagccgg tagtagaggt cttcgcggaa gtggccggtc gccaccatcg
      601 acgccaggtt cctgttggtg gcggcgatca gccgcacgtc gaccttcagc gtgcgggtgc
      661 cccccagccg ctcgaactcg tgctcctgga gcacgcgcag gatcttcgcc tgcgtgctcg
      721 ggctcatgtc gcccacctcg tccaggaaca tcgttccgcc gtcggcctgt tcgaaccgcc
      781 cgacgcgctg cttgtcggcg ccggtgaacg cccccttctc gtggccgaac agctccgact
      841 cgaggaggtt ctcctgcagc gccgcgcagt tcacccgcac gaagttgcgc gaggcgcgca
      901 gcgagttgtg gtggatggcc ccggcgatca gctccttgcc ggtgcccgtc tcgccgcgga
      961 tcaggacggt cgtgttgctc ttggccacct tgcggacgac gccgagcacg cgcgccaggg
     1021 cgccgctgct gccgacgatc cggtcgaact cgtagatgtc gttctgggtg tgccggaggt
     1081 agtcgagctc gttccgcatc cggcgcagct cgagcgcctt ctcgatcttc acctccatct
     1141 cttcgatctc gaacggcttc tgcacgtagt cgaaggcgcc gctcttcatc gcctcgaccg
     1201 ccgtgttcac cgagccgaac gcggtcatca ggatgacgga ggtcgagggg tgcagcgact
     1261 tggtggtgcg cagcacgtcg aggccgtcgc tgccccccat cttcaggtcg ctcagcacca
     1321 cgtcgaagta gctctcgtgc agccgctcga tcgcggcgtt cccgttgggc gcctcgtcca
     1381 cctcgtggcc cgcctcggtc agcgcgcgcg cgagcccgcg gcggagcgcg tcgtggtcgt
     1441 cggcaatcag gatgcggccc atcttctctc ttcctgtcaa aacggcgctc gtgtacggcc
     1501 ggcacccgcc ttcgcctcgc gcggcgaggc aggcctaccg cagccagccc tcgccggcct
     1561 ccatcggcag cgtgacgcga aagtgcgtcc cctggcctgc cgcagaggtg aagtcgatgt
     1621 gcccgttgtg ggcgtccacg atcttccgga cgatcgccag gccgaggccc gagccctgcg
     1681 gcttggtggt gaagaacggg ttgaacacgc ggtcgaggat ctcggggctc acccccgggc
     1741 cgctgtcggc cacgtccacg accacggtcg gggggcccgg ccgccggggc tcggcgccgg
     1801 gctcgacgta gccctcgccc tggaccgccg cgatcgcgat ggtcccggtg ccgttcagcg
     1861 cctcgagcgc gttgatgatc aggttgctga agacctgcac cagctggtgg tggtcggcct
     1921 ggatcagcgg cagctgctcc tgcacgttca gcgtgacgtt caccccgccg cggctcacct
     1981 tgcgctcggc cgtggtcacc gcgtcgtgca gcacctgggg gatcgacgtc cgctccacct
     2041 gcaggcggat cggccggacg aagtcgagca cctcgagcac gatcgcgttg gccaccttcg
     2101 cctcggcgat gatgtcgttg atcagcccct gggcctcagg cgagtcggga atgcggcggc
     2161 gcagcagccc ggccatcacc tcgatccccg ccagcgggtt cttcacctcg tgggcgatgg
     2221 cggccgccat ctcgccgagc gcggcgaggc ggtcgcgcag gcgctcgcgc tcctcgagct
     2281 gctcgaccag cgtcaggtcc ttgaagaaca gcgacgcccc caccacgtcg cccgcgtcgt
     2341 cgaggacgtg cgacagcgtg tagccgatca ccttgcccga cgtcctcagc ttcagctcgg
     2401 cgcggttcgg caggttctgc agctcgaagg ccgcccccag gatccggatg acgtcgggtt
     2461 ggtcctgcag gacgtccgtg tacggggcgc cgatgtcgtc gggcgtcggg tcgatgttga
     2521 agacccggta agcgacctcg ttcatcgcca ccacgcggcc gtcccgcgcg atcgcgagga
     2581 cgccgttccg catgctcccg accagctggc ggaagaagcg gtcgccgacg ggggggagcg
     2641 acggctgccc cggggtagcg gggtgcgcgc ccgagcgcgg cttgggcggc gccggcgtcg
     2701 gctgtgggtc agtcatgacg tggacggggg agccgaagaa ccgatgcgat ttcaatagtg
     2761 taaccatcct gggcccatgt gccaagcgaa ggccgcgcgc accgcagccc atgaatgggc
     2821 cgcaaacggc atgcctgagc ctcagccagg ggatgttggg ggaaactggc aggaaatcga
     2881 gcgtcgaggc acgcgccggc ggctctcctc gatacgattt tgtaattcaa ccctcaaggc
     2941 agggctggtg cggccggacc gaaccgcgcg acccgtcagg actgtaagtc ctgctcggcg
     3001 cgctcgaggg agtgttggag ctcttcccac ttgtgcatca ggtctccgac ctcccacatc
     3061 agggcctggt gccgatcgac gacgggctga acggcctcgc ggttgtcgta gaacccgtcg
     3121 ctcgccatct gggcctcgag cgccttgatc tcggcctcgc gcgcggcgat ggtctcctcc
     3181 agctcggtga cccgggttcg gagcgccttc tggtgacgct cgcggcggcg cgcctcgctc
     3241 aacgcctgtc tccggcgcgc ctcgcgctcg gcgctcgacg gctcgccgcg tccgtcagac
     3301 tttgccgtcg cggccgtcgc ggccgccccc ggcgcgacgg cggcgatcgc ctcgacgcct
     3361 ggcttgccgg gccgcggcgg cgatggctgg ttggccggcg cgggaccctt ccgctgtccc
     3421 ttgctccaga ggaactcgtc gtagcgtccc gggtagctgt gggcctcgcc tccgccgatc
     3481 tcgacgaccc ggttggccag cttctcgacg aagtagcggt cgtgcgagac gaagatgagc
     3541 gtgccgccgt agtcctcgag cgcctcgagc aaaacgtcct tcgagtcgag gtcgaggtgg
     3601 ttggtcggct cgtccagcag cagggtgttc gaggggcgca gcagcatgcg ggcgacggcc
     3661 agccgggtgc gctcgccgcc cgagagcacc tccaccttct tgtacacgtc gtcgccggag
     3721 aagaggaagc cgccaaggat gttgcggatg gcgggcacca tgtgcaccgg cgagcccgac
     3781 tgcagcgttt cgtacaccgt cagggcgggg tcgaggcgca cggcctcgtc ctgggcgaag
     3841 tactgcatca cgacctggtg gccctcgcgg cgggcgcccg agtcgggccg ctcgacgccg
     3901 gcgagcattc gcatcagggt cgacttgccg cttccgttgg ggccgacgag cgcgatccgg
     3961 tcgccgcgct ccacgtgcag gttggcgcgt tcgaagacga cggtggggcc gtacgccttc
     4021 cggatccgct cgagctcgag caccgtgcgg ccgctcttca ccgaggaggg gaagctgaag
     4081 tgcacgcgct tgcgctcggg cggcacctcg atcgggacga ccttctcgag catcttcacc
     4141 cggctctgca cctgggccgc cttggtggcc ttgtagcgga accggctgat gaacatcttg
     4201 atccgctcga tctcctcgtc ctgctgcttc tttgcctcgc gcacccgctc gaggcgcgcc
     4261 tggctctccg cgaggtaccg ggtgtagttg cccgggtagt cgttgagctg ccgcaggtag
     4321 acctcggcca cccggttgat caccgtgtcg aggaagtagc ggtcgtgcga cacgaggatg
     4381 acggcgtgcg ggtagtcgtg gaggtactcc tcgagccagt tgcgggcctc gaggtcgagg
     4441 tggttggtcg gctcgtcgag cagcagcagg ttcggccggg cgagcagcag cttgccgagc
     4501 gcgagccgca tctgccagcc gcccgagaag gtctcggtgg ggcggtcgag gtcggcatgg
     4561 ctgaagccga ggccctgcag gacggtgccg accttcaggt ccatgttgta gccgtcgtgc
     4621 aggcggaagc ggtcctggag gtcgctgtag cggacgagca tctgctcgtg atcggcgtcg
     4681 gaaacgtcgg ggtggccgag ccgctcctcg agggcctcca tctcggcgcg gacctcgagc
     4741 agggggccga aggcgagcat cgcctcctcg cggaccgtgc gcccctggtg ggcgatgccg
     4801 tcctggggga ggtagccgac ggtgaggtcg gccggtttga cgacctgccc cgagtcgggc
     4861 tcctcgaggc cggcgaagat cttgaggagg gtggtcttgc ccgatccgtt cgggccagtg
     4921 aggccgacgc gctcgccgtc ggagatttgc caggtgacgt tgtcgaagag cacccggtcg
     4981 ccgaaagact tcgtgaggcc tgcgatctgg atcatcggct cgaaaggtcc cgccttcgtg
     5041 aaccccttat tgtaccagta gcacaggcgt cccgcctgtg ctgcgtccca gtaggacagg
     5101 cgtctcgcct gtcggggtcc cgtcccagta ggacaggc
//
LOCUS       DAOPQX010000261         5121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17742_length_5121_cov_3.895973, whole genome shotgun sequence.
ACCESSION   DAOPQX010000261 DAOPQX010000000
VERSION     DAOPQX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17742_length_5121_cov_3.895973"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKK95_12750"
     CDS             <1..382
                     /locus_tag="PKK95_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOC19136.1"
                     /translation="TILTDPNFLHRGEQVPLVGPDEPMREVVREMSRKGLGMTCVGTD
                     GRLAGVITDGDLRRHMAGANFLDSPARAVMTPDPATVSRTMLAVEALALMERRKITSL
                     VVVTGEGRIEGVVHLHDLWRTGMF"
     gene            398..946
                     /locus_tag="PKK95_12755"
     CDS             398..946
                     /locus_tag="PKK95_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit delta"
                     /protein_id="HOC19137.1"
                     /translation="MPSDDTTLQRQAARVRLLLFDVDGVLTDGRILVHPDGTESKSFS
                     IRDGTGIVWAHRAGLRTGLLSGRHSPATAVRAGQLGIPIVRHQRGDKLEVFEGLLRDE
                     GTEAEAVAFMGDDLLDLPVLLRVGLSAAPADADPEVVRRVDWVSRHPGGRGAARELIE
                     LVLRAQGKWDGVLEGYLGGTRV"
     gene            943..>2161
                     /locus_tag="PKK95_12760"
     CDS             943..>2161
                     /locus_tag="PKK95_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19138.1"
                     /translation="MNEYVPMLVGLVALLAGLTVGKAWERYKLRDGRWFDRRKARQTP
                     HYMLGLNFLGANQPDLAIEELTKAAAADSDALEIDMILGNLYREKGQVGRAVHVHQGL
                     LQRPKLTRLEHAYALLCLGLDFKRGGFVDRAHEAFNEVLRLDPDNEYALANLEKLYEE
                     QHQWEEAYQVRRRLAGPKGPEPETPHAQILAFLENELGVDAVKRDDLKAAAARFRAAI
                     DHDPRTVPAYLHLGDVHHRQGQLAEAAEIWERLLEVAPDRAYLAFDRLEPVYAALGTP
                     QRFAEQCRRLIAANAQDWRARRALAQHVAARGEAAEALELLLEAVVHNPHSLSLHQTI
                     WQVLRQLGLEPSLVDRYVELTGQSVFYADPHVCVRCRYRSTELLWQCPHCHEWNSFLE
                     ERMEPAKDAEQAEG"
     assembly_gap    2162..2261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2367..2954)
                     /gene="coaE"
                     /locus_tag="PKK95_12765"
     CDS             complement(2367..2954)
                     /gene="coaE"
                     /locus_tag="PKK95_12765"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOC19139.1"
                     /translation="MRRIALTGGIATGKSYVRRRFEALGLPAIDADAVVHGLLGPGPA
                     ASRVAGRFGNAFLDAAGGIDRRALGRLVFSDQSARADLEAILHPLVYERIAAWMAERQ
                     AAGAAAALADIPLLFETGHEGDFDVVVVAACDPEEQVRRVMARDKLDEAAARDRLAAQ
                     WPIARKAAAADHVIQTGGTFEETDRQVDALYRLLA"
     gene            complement(2992..3915)
                     /locus_tag="PKK95_12770"
     CDS             complement(2992..3915)
                     /locus_tag="PKK95_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOC19140.1"
                     /translation="MPATVLDGAEVARQIRNELRAKVEAFTASRKRPPGLGIVLVGHD
                     PGSEVYVRNKLKSAGETGLRADLHRLPEDASLPEVVAAVRELNASGAHDGILVQSPLP
                     AALGPEAEQVVFDEVDPAKDVDGFHPVNVGRLVQGRPFLVPCTPLGILELLDRTGTQI
                     KGRHAVVIGRSDIVGKPVALLLLHRHATVTICHSRTVDLPKVAATADVLVVAIGRAGF
                     VTRDFVKPGATVIDVGMNRVDDREQARKLFPEGHSRLADFERRGSVLVGDVHPEVWEV
                     AGALTPVPGGVGPLTIALLLSNTLKAAEARS"
     gene            4016..>5121
                     /locus_tag="PKK95_12775"
     CDS             4016..>5121
                     /locus_tag="PKK95_12775"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOC19141.1"
                     /translation="MASRGGFSSRVGFILAGAGSAIGLGAVWRFPYMTGANGGAVFVL
                     VFLACCFLIGVPVMIAEITLGRHTERNPVGTFNLLHGGRLWKAVGALCVLSGFMIFSW
                     YSVIAGWVLGYLWEAVRGTFSTAHTADEVGRFFAAYVSDAPTVLFLHFLVIAATSGIV
                     LQGIKGGIERLSKWLMPVLFVLLILLAIRSVTLPGAGAGLEFYLRPDFSKVNARMLLA
                     AMGQALFSLSIGMGTMLTYGSYLAKREDIPAAAMWITFFEVLISIIAGFAVLPAVSAM
                     GQSFTEGPRLIFVVLPSIFAMMPGGYLFGVGFYVLVIIAVLTSTVSILEVPVAYFVDE
                     RGWSRRAATLFVSGVAFVLGIPAGLAFGASAFFT"
BASE COUNT          712 a         1773 c         1790 g          746 t
ORIGIN      
        1 caccatcctg accgatccca acttcctgca ccgcggcgag caggtgcccc tggtcggtcc
       61 cgacgagccg atgcgcgagg tggtccgcga gatgtcgcgc aaggggctcg ggatgacctg
      121 cgtgggcacg gacggccggc tggcgggtgt gatcaccgat ggcgatctgc gccggcacat
      181 ggccggcgcg aacttcctcg acagccccgc gcgcgcggtg atgacaccgg acccggccac
      241 ggtgtcccgc acgatgctcg ccgtcgaggc gctcgcgctg atggagcgcc gcaagatcac
      301 ctcgctcgtc gtcgtgaccg gggagggccg catcgagggg gtggtgcacc tccacgatct
      361 gtggcggacc gggatgttct gaggccttgg aggacacttg ccgagcgatg acacgaccct
      421 ccagcgacag gcagcccgcg tccggctgct gctgttcgac gtggacggcg tgctgaccga
      481 cggccgcatc ctcgtgcacc ccgacggtac cgagtcgaag tcgttctcca tccgcgacgg
      541 caccggcatc gtgtgggcac accgggcggg gctccgcacc gggctgctgt cgggccgcca
      601 ctcgccggcg acggccgtcc gggccgggca gctcggcatc ccgatcgtcc gccaccagcg
      661 cggggacaag ctcgaggtct tcgaggggct gctgcgcgac gaaggaacgg aggccgaggc
      721 ggtggcgttc atgggggacg acctgctcga cctgccggtc ctgctgcgcg tggggctgtc
      781 ggccgcgccg gccgacgccg accccgaggt cgtgcggcgg gtcgactggg tgagccgcca
      841 cccgggcggg aggggcgccg cccgcgagct gatcgagctg gtgctgcggg cacagggaaa
      901 gtgggacggg gtgctggaag ggtacctggg cgggacgcgg gtatgaacga gtacgtgccg
      961 atgctcgtcg gcctcgtcgc gctgctggcg ggcctcaccg tcggcaaggc gtgggagcgc
     1021 tacaagctgc gcgacggccg gtggttcgac cgtcgcaagg cgcgccagac cccgcactac
     1081 atgctggggc tgaacttcct cggggcgaac cagcccgacc tggcgatcga ggagctgacc
     1141 aaggcggcgg ccgccgacag cgacgccctc gagatcgaca tgatcctggg caacctgtac
     1201 cgggagaagg gccaggtggg ccgggccgtc cacgtgcacc aggggctgct ccagcggccg
     1261 aagctgacgc gcctcgaaca cgcctacgcc ttgctctgcc tcggcctcga cttcaagcgc
     1321 ggcgggttcg tggaccgcgc gcacgaggcg ttcaacgagg tgcttcgcct cgacccggac
     1381 aacgagtacg cgctcgccaa cctcgagaag ctctacgagg agcagcacca gtgggaggag
     1441 gcctaccagg tgcggcgccg cctcgcagga ccgaagggcc ccgagcccga gacgccgcac
     1501 gcccagatcc tcgcgttcct cgagaacgag ctgggcgtcg acgcggtaaa gcgcgacgac
     1561 ctgaaggcgg cggccgcgcg cttccgcgcc gcgatcgacc acgacccgcg gaccgtgccc
     1621 gcttacctcc acctgggcga cgtgcaccac cgccaggggc agctggccga ggccgcggaa
     1681 atctgggaac ggctcctcga ggtcgccccc gaccgcgcct acctcgcctt cgaccgcctc
     1741 gagccggtct acgccgcgct cggcacgccg cagcggttcg ccgagcagtg ccgccggctg
     1801 attgcggcca acgcgcagga ttggcgcgcg cggcgcgcgc tcgcccagca cgtcgcggcc
     1861 aggggcgaag ccgccgaggc gctcgagctg ctgctcgagg ccgtggtgca caacccccac
     1921 agcctgagcc tgcaccagac catctggcag gttcttcggc agctcgggct cgagccgtcg
     1981 ctggtcgacc ggtacgtcga gctgaccggg cagtcggtct tctacgccga cccgcacgtg
     2041 tgcgtacgct gccgctatcg cagcaccgaa ctgctgtggc agtgcccgca ctgccacgag
     2101 tggaacagct tcctggaaga gaggatggag cccgccaagg acgccgagca ggcggaaggc
     2161 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagcttcctg gaagagagga
     2281 tggagcccgc caaggacgcc gagcaggcgg aaggctgacc ccagcccacc gctaacggcc
     2341 ctgctcgccc caagcgagcg ccggggtcag gcgagcagcc ggtagagggc gtccacctgc
     2401 cggtcggttt cctcgaacgt gccgccggtc tggatcacgt ggtcggccgc cgcggccttg
     2461 cgcgcgatcg gccactgcgc cgcgagcctg tcgcgggccg cggcctcgtc cagcttgtcg
     2521 cgcgccatca cccgccgcac ctgctcctcg gggtcgcacg ccgccaccac caccacgtcg
     2581 aagtcgccct cgtgcccggt ctcgaagagc agcgggatgt cggccagcgc ggcagccgcg
     2641 cccgccgcct ggcgctcggc catccatgcc gcgatgcgct cgtaaacgag cgggtgcagg
     2701 atcgcctcga ggtcggcacg ggccgactgg tcgctgaaga ccaggcggcc gagcgcccgg
     2761 cgatcgattc cgccggccgc gtcgaggaag gcgttgccga agcgcccggc gacgcgcgaa
     2821 gcggcgggcc cggggccgag caggccgtgg acgaccgcgt ccgcgtcgat ggcgggaagg
     2881 ccgagtgcct cgaaccgcct gcgcacgtag ctcttgccgg tcgcgattcc gccagtgagg
     2941 gcgatgcgtc gcatggatta cgccgggatg ccgggtaatg ccccgccgcg atcaggagcg
     3001 ggcttcggcc gccttcaacg tgttcgacag cagcagcgcg atcgtgagcg gaccgacgcc
     3061 gccgggcacg ggggtcaggg cgccggccac ctcccacacc tccgggtgaa cgtcgccgac
     3121 cagcaccgag ccccgccgct cgaagtcggc caggcgggaa tgcccctcgg ggaacaactt
     3181 ccgcgcctgc tcccggtcgt ccacccggtt catgccgacg tcgatcaccg tcgccccggg
     3241 cttcacgaag tcgcgggtga cgaacccggc gcgcccgatg gcgacgacca ggacgtcggc
     3301 cgtcgcggcc accttcggga gatcgaccgt ccgcgagtgg cagatcgtga cggtggcgtg
     3361 gcggtgcagc agcagcagcg ccaccggctt gccgacgatg tcgctgcggc cgatcaccac
     3421 ggcgtggcgc cccttgattt gcgtcccggt gcggtcgagg agctccagga tgccgagcgg
     3481 cgtgcagggc accaaaaacg gccgcccctg cacgaggcgc ccgacgttga cggggtggaa
     3541 cccgtccacg tccttggcgg gatcgacctc gtcgaacacg acctgctcgg cctccggccc
     3601 gagcgcggcg ggcaagggcg actgaacgag gatgccgtcg tgcgcgccgc tcgcgttgag
     3661 ctcacggaca gccgcgacga cctcggggag cgaggcgtcc tcgggcaggc ggtggaggtc
     3721 ggcgcgcagg cccgtctccc ccgccgactt cagcttgttg cggacgtaga cctcggagcc
     3781 cgggtcgtga ccgaccagca cgatcccaag ccccggcggc cgctttcgcg aggcggtgaa
     3841 cgcctcgacc ttcgcgcgca actcgttcct gatctgtcgg gcgacttcgg cgccatcgag
     3901 cacggtggca ggcatacgcc tcagagcata accgatcgac gcgtggtagt atgccgcggc
     3961 gaaaggtgag gctgtcccct tccccacctt ctcaccttct tctcgaggga ggcgcatggc
     4021 gagccgggga ggtttcagct ccagggtcgg gttcatcctc gcgggggcgg gttcggcgat
     4081 cgggctcggc gccgtctggc gcttccccta catgacgggc gccaacgggg gcgccgtctt
     4141 cgtcctcgtg ttcctcgcct gctgcttcct gatcggcgtg ccggtgatga tcgccgagat
     4201 cacgctcggg cggcacaccg agcgaaaccc ggtgggcacc ttcaacctgc tgcacggcgg
     4261 gaggctgtgg aaggccgtcg gcgcgctgtg cgtgctcagc ggcttcatga tcttctcctg
     4321 gtacagcgtg attgcgggct gggtgctcgg ctacctgtgg gaggcggtcc ggggcacctt
     4381 cagcacggcg cacacggccg acgaggtggg ccggttcttc gccgcctacg tgagcgacgc
     4441 gcccacggtg ctgttcctgc acttcctcgt catcgccgcg acgtccggca tcgtgctgca
     4501 gggcatcaag gggggaatcg agcggctgtc gaagtggctg atgcccgtcc tcttcgtgct
     4561 gctgatcctg ctcgcgatcc ggtcggtgac gcttcccggc gccggggccg gcctggagtt
     4621 ctacctccgg cccgacttct cgaaggtgaa cgcgcggatg ctgctcgcgg ccatgggcca
     4681 ggcgctgttc tcgctgagca tcggcatggg gaccatgctg acctacggga gctacctggc
     4741 caagcgcgag gacatcccgg ccgcggcgat gtggatcacc ttcttcgagg tcctcatctc
     4801 gatcatcgcc gggttcgcag tgctgccggc ggtctccgcg atgggtcaga gcttcaccga
     4861 ggggccgcgc ctgatcttcg tggtcctgcc gtcgatcttc gccatgatgc cgggcggcta
     4921 cctgttcggc gtcggcttct acgtgctcgt gatcatcgcc gtgctcacct ccacggtctc
     4981 gatcctcgag gtcccagtgg cgtacttcgt cgacgagcgc ggctggtcgc gccgggcggc
     5041 gaccctcttc gtctcgggag tcgccttcgt gctcggcatc cccgccggcc tcgccttcgg
     5101 ggccagcgcg ttcttcacca g
//
LOCUS       DAOPQX010000262         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17805_length_5107_cov_3.793349, whole genome shotgun sequence.
ACCESSION   DAOPQX010000262 DAOPQX010000000
VERSION     DAOPQX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17805_length_5107_cov_3.793349"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..433
                     /gene="ndk"
                     /locus_tag="PKK95_12780"
     CDS             2..433
                     /gene="ndk"
                     /locus_tag="PKK95_12780"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOC19142.1"
                     /translation="MESTLAIIKPDAVGRRLTGRILARIEEAGFQIRAMRMVQLTRDE
                     AEGFYAVHRGKPFFPGLTEFMSSGPAVVLVLEAPGAIGRWRELMGATDPSKAAEGTLR
                     REYGTSIGENATHGSDAPETAAFEIGYFFRGVELARAGRLG"
     gene            444..638
                     /locus_tag="PKK95_12785"
     CDS             444..638
                     /locus_tag="PKK95_12785"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOC19143.1"
                     /translation="MGKLLIVIGLAIAAVGLAVSAGLPLGRLPGDFVFRRGPVTFYFP
                     LTTAVLLSVLLTVLLALLRR"
     gene            675..1952
                     /gene="hisS"
                     /locus_tag="PKK95_12790"
     CDS             675..1952
                     /gene="hisS"
                     /locus_tag="PKK95_12790"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOC19144.1"
                     /translation="MIPAVRGTRDILPGESERWAFVEETARRLFEQYGYFEIRTPIIE
                     REELFAKGTGETTDIVQKEMYSFTDKGGERITLRPEATPSLVRAFVEHALEQSMAVPK
                     VYTLGPMFRYERPQKGRYRQFHQLDVEVFGVKDPSVDGEVIDLAWALVRALGIGNVEL
                     AINSVGCSECRPAFTAALVEALGRDVEKLCADCRRRAETNPLRIFDCKVPADQPIIDR
                     LPHTVDYLCADCRAHFDAVRADLDVYGIPYHVSHRLVRGLDYYTRTTFEVLAGHLGAQ
                     NAVLGGGRYDGLVKQLGGPDRVGIGFAAGIERLVLALPAELPVTPPVPDAFIAALGEP
                     ARREALALLRDLRRAGLNAQLDYEGRTLKSQMKRADRLGAPLVLILGEDELARGEVTV
                     KRMATSTQEPVPRHEAVALVARERAARGAPATR"
     gene            1960..3735
                     /gene="aspS"
                     /locus_tag="PKK95_12795"
     CDS             1960..3735
                     /gene="aspS"
                     /locus_tag="PKK95_12795"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOC19145.1"
                     /translation="MIEPLGNWTRTHTCGALRPAHIGDTVTLLGWVHRVRDLGSLVFI
                     DVRDREGLTQVVVQEGRAFLEAAKRLRAEFVVGIQGTVRARGEVNPRIATGEVEVEAS
                     DLRVLNEAAVPPFQIGDEPSASEELRLRYRYLDLRRPRLQANLGLRHRVTMAVRRALD
                     AQGFWEIETPVLGKSTPEGARDYLVPSRVHRGSFYALPQSPQLFKQLLMVAGTDRYFQ
                     IVKCFRDEDQRADRQPEFTQVDIEMAFARPDTVFALIEPLMRDVMAVIGREVPTPFRR
                     IPYPEAIARYGSDKPDLRCGMEINDLSALFAGTAFQPFREALESGGAVRGLAVAGGAR
                     YSRKQLDRLGEQAKETGARMLVWARSGPDGLKSSALKALGEEAVRHALAAAGAGEGDL
                     LLMAAGEPDAVSKALGQLRLVVAREEGLLKPDTFAFTWVTGFPLLEWDRDENRFVAMH
                     HPFTSPVPEDERLLEQEPARVRAQAYDLVLNGSELGGGSIRIHDPGLQARVFRAIGLE
                     EAEAKRRFGFFLEALRYGTPPHGGIALGLDRMVALLAGEPSIREVMAFPKSAAAMDLM
                     TGAPSTVDESQLRDLGLRVEPGRAD"
     gene            3815..4786
                     /locus_tag="PKK95_12800"
     CDS             3815..4786
                     /locus_tag="PKK95_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOC19146.1"
                     /translation="MRKIVVPDEGIETLFGSHDENLRFLETLLNVTIRTHGHDLLVEG
                     DDANTEKVGRVVSGLASLAREGYRIGNGDVKTAAQLVSERPDVDLHDYFFKGAPARTT
                     GRRQVAPKSVNQRRYLDLIDQNDIVFGIGPAGTGKTYLAMAQAVSFLLAKKVARIILA
                     RPAVEAGEKLGFLPGDLQEKVNPYLRPLYDALYDMLDVERVGRLLERGTIEIAPLAFM
                     RGRTLNDSFVILDEAQNTTSEQMKMFLTRLGFGSKAVITGDITQIDLPAGHTSGLIEA
                     MKVVSAIEGISFVYFDERDVVRHKLVQQIVKAYEAFSNGSTSVRRIS"
     gene            4796..>5107
                     /gene="ybeY"
                     /locus_tag="PKK95_12805"
     CDS             4796..>5107
                     /gene="ybeY"
                     /locus_tag="PKK95_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOC19147.1"
                     /translation="MPDEDQPPGLQLSLTDGSGRPARAPGLSAWLKRVAPSRARGGVS
                     IALVSDAAMRRLNREFAGVDRATDVLSFPAAASPRAPGAAPLLGDVVIATGVARRQAQ
                     EA"
BASE COUNT          760 a         1749 c         1839 g          759 t
ORIGIN      
        1 tatggagagc acgctggcca tcatcaagcc cgacgcggtc gggcggcggc tgaccgggcg
       61 aatcctcgct cgaatcgaag aggcaggttt ccagatccgt gccatgcgca tggtccagct
      121 gacgcgcgac gaggcggagg gcttctacgc ggtgcaccgg ggcaagccgt tctttccggg
      181 gttgaccgaa ttcatgtcgt cgggccccgc cgtcgtgctc gtgctcgagg ccccgggcgc
      241 gatcggccgc tggcgcgagc tgatgggggc caccgatccc tccaaggcgg ccgaagggac
      301 gctgcggcgc gagtacggca catcgatcgg cgagaacgcg acgcacggct cggacgcccc
      361 cgaaacggcg gccttcgaga tcggctactt cttccgcggg gtggagctgg cccgggcggg
      421 ccgcctgggc tgacggggtg tcgatgggga agctgctgat cgtcatcggc ctggccatcg
      481 ccgcggtcgg cctggcggtc agcgccgggc tgccgctcgg ccggctcccc ggcgatttcg
      541 tgttccggcg cggccccgtg accttctact ttccgctgac gaccgccgtg ctgctgagcg
      601 tgctgctcac ggtgctgctg gccctgctcc ggcggtgact gggctcgcgg cgcggcaagc
      661 aaccaggtcc tcacatgatt ccagcggttc gcggcaccag ggacatcctg ccgggcgagt
      721 cggagcggtg ggccttcgtc gaggagaccg cgcggcgcct gttcgagcag tacgggtact
      781 tcgagatccg cacgccgatc atcgagcgcg aggaactctt cgccaagggc accggggaaa
      841 ccaccgacat cgtccagaag gagatgtact cgttcaccga caaggggggt gagcggatca
      901 ccctgcggcc ggaggcgacg ccaagcctcg tccgggcctt cgtcgagcac gcgctcgagc
      961 agtcgatggc cgtgcccaag gtgtacacgc ttggcccgat gttccggtac gagcggcccc
     1021 agaagggccg gtaccgccag ttccaccagc tcgacgtcga ggtgttcggg gtgaaggacc
     1081 cctcggtgga cggcgaggtg atcgacctgg cgtgggcgct ggtgcgggcg ctcgggatcg
     1141 ggaacgtcga gctggcgatc aactcggtcg gctgctccga gtgccggccg gcctttaccg
     1201 ccgcgctcgt cgaggcgctc ggccgcgacg tggaaaagct gtgcgctgac tgccggcggc
     1261 gcgccgaaac caatccgctg cggatcttcg actgcaaggt gcccgcggac cagccgatca
     1321 tcgaccggct cccgcacacc gtcgactacc tgtgcgccga ctgcagggcg cacttcgacg
     1381 cggtccgcgc cgacctcgac gtctacggaa ttccgtatca cgtctcgcac cgcctggtcc
     1441 ggggcctcga ctactacacg cggacgacct tcgaggtgct cgccggacac ctcggcgcgc
     1501 agaacgctgt gctcggcggc ggacggtacg acggcctggt caagcagctc ggcggccccg
     1561 accgcgtggg gattgggttc gcggccggga tcgagcgcct ggtcctggcg ctgcccgccg
     1621 aactccccgt gacaccgccg gtccccgacg cattcatcgc cgcgctcggc gagccggctc
     1681 gccgcgaggc gctggcgctg ctgcgcgacc tccgccgggc cgggctgaac gcgcagctcg
     1741 actacgaggg gcgcacgctg aagtcccaga tgaagcgcgc cgatcggctc ggcgccccgc
     1801 tcgtcctgat tctcggcgag gacgagctgg cgcgggggga ggtgaccgtc aagcggatgg
     1861 ccacctcgac ccaggaaccc gtgccgcgcc acgaggccgt cgcgctcgtg gcccgtgagc
     1921 gcgccgcccg aggggcgccc gccacccgat gaggaccaga tgatcgaacc gctcggcaac
     1981 tggacccgga cgcacacgtg cggtgcactg cggcccgccc acatcggcga cacggtgacg
     2041 ctgctcggct gggtgcaccg cgtgcgcgac ctggggtcgc tcgtcttcat cgacgtgcgc
     2101 gaccgcgagg gcctcacgca ggtcgtcgtc caggaagggc gcgcgtttct cgaggcggcc
     2161 aagcggctgc gcgccgagtt cgtcgtcggt atccagggaa ccgtccgcgc ccgtggagag
     2221 gtgaatccac gaatcgcgac cggggaggtc gaggtcgaag cctccgatct gcgcgtgctg
     2281 aacgaggccg cggtccctcc cttccagatc ggcgacgagc cgagcgcgtc ggaggagctg
     2341 cgccttcggt accgctacct cgatctgcga aggcctcgcc tgcaggccaa cctcgggctc
     2401 cgccaccggg tgaccatggc ggtgcggcgc gcgctcgacg cgcaggggtt ctgggagatc
     2461 gagacgccgg tgctggggaa gtccaccccc gagggggcgc gcgactacct ggtcccgagc
     2521 cgcgtgcacc ggggcagctt ctacgcgctg ccgcagtcgc cgcagctgtt caagcagctg
     2581 ctcatggtcg ccgggaccga tcgctacttc cagatcgtca agtgcttccg tgacgaggat
     2641 cagcgggccg accgccagcc cgagttcacg caggtggaca tcgagatggc cttcgcgcgg
     2701 cccgacacgg tgttcgccct gatcgagccg ttgatgcgcg acgtgatggc ggtcatcggg
     2761 cgggaggttc cgaccccctt ccgccggatc ccgtacccgg aggcgatcgc caggtacggc
     2821 tccgacaagc ccgatctgcg ctgcgggatg gagatcaacg atctgtcggc gcttttcgcc
     2881 ggcaccgcct tccagccgtt ccgcgaggcg ctcgagtctg ggggggcggt gcgggggctg
     2941 gccgtcgccg gcggcgcccg gtacagccgc aagcagctcg atcggctcgg cgagcaggca
     3001 aaagaaacgg gcgcacggat gctggtctgg gcgcgaagcg ggccggacgg cctcaagagc
     3061 tcggcgctca aggcgctggg cgaggaggcg gtccgccacg cgctcgcagc ggccggggcg
     3121 ggtgagggcg acctgctgct gatggcggcg ggggagcccg acgccgtgtc gaaggcactc
     3181 ggtcagctgc ggctggtggt cgcgcgcgaa gagggcctgt tgaagcccga cacctttgcg
     3241 ttcacctggg tcaccggctt tccgctgctc gagtgggatc gcgacgagaa ccggttcgtc
     3301 gccatgcacc acccgttcac ctctcccgtg cccgaggacg agaggctgct cgagcaggag
     3361 cccgcccggg tgcgggcgca ggcctacgac ctggtgctca acggcagcga actcggcggc
     3421 ggcagcatcc gcatccacga ccccgggctg caggcgcggg tcttccgggc catcggcctc
     3481 gaggaggccg aggcgaagag gcggttcggc ttcttcctcg aggcgctgcg ctacggcacc
     3541 ccgccgcacg ggggcatcgc cctgggcctc gaccggatgg tggccctgct ggccggagag
     3601 ccgtcgatcc gcgaggtgat ggccttcccg aagagcgccg cggcgatgga cctgatgacc
     3661 ggggcgcctt ccacggtgga cgagagtcag ctgcgcgatc tcgggctgcg ggtggagccc
     3721 ggccgtgcgg attgaccgcc cccgggggcg gatggtacaa tcggctgcct aactctctcg
     3781 tttcaaagga gatgcgcacc gcccagcggt cacgatgaga aagatcgtgg tccccgacga
     3841 ggggatagaa acgcttttcg gctcgcacga cgagaacctt cggtttctcg agaccctcct
     3901 caacgtcacc atccgcaccc acggccacga cctgctggtt gagggggacg acgccaacac
     3961 cgagaaagtg gggcgcgtcg tctcggggct cgcctcgctc gcccgcgagg gctaccgcat
     4021 cggcaacggc gatgtgaaga cggccgccca gctggtgtcg gagcgtcccg acgtcgatct
     4081 gcacgactac ttcttcaagg gcgcccccgc gcggacgacc gggcgccggc aggtggcgcc
     4141 caagtcggtc aaccagcgcc gctacctcga cctgatcgac cagaacgaca tcgtgttcgg
     4201 gatcggaccg gcaggaacgg gcaagacgta cctggcaatg gcgcaggcgg tgtcgttcct
     4261 cctcgcaaaa aaggtcgcgc ggatcatcct cgcccgcccg gcggtggagg ccggcgagaa
     4321 actgggcttc ctgccgggcg acctgcagga gaaggtgaac ccgtacttgc ggccgctcta
     4381 cgacgcgctg tacgacatgc tcgacgtcga gcgggtcggg cgcctgctcg agcgcgggac
     4441 catcgagatc gcgccgctcg cgttcatgcg cgggcggacg ctcaacgact cgttcgtgat
     4501 cctcgacgag gcgcagaaca ccacctccga gcagatgaag atgttcctga cgcgcctcgg
     4561 cttcggctcg aaggcggtga tcaccgggga catcacgcag atcgacctgc cggccgggca
     4621 cacctccggg ctcatcgagg cgatgaaggt cgtttcggcc atcgagggca tctccttcgt
     4681 ctacttcgac gagcgggacg tcgtccggca caagctggtg cagcagatcg tgaaggcgta
     4741 cgaggcgttc tcgaacggga gcacctccgt gcgccgcatc tcctgacgag ctgtcatgcc
     4801 cgacgaagac caacctccag gcctccagct ttccttgacg gacgggtccg gccggccggc
     4861 gagagccccg gggctgtcgg cgtggctgaa gcgggtcgcg ccgtcgcgcg cccggggcgg
     4921 cgtgtcgatc gcgctggtgt ccgacgcggc gatgcgtcgc ctcaaccgcg agttcgcagg
     4981 agtcgaccgg gctaccgacg tcctgtcgtt cccggccgcc gcctcgccgc gcgcgcccgg
     5041 ggccgcgccg ctgctgggcg acgtcgtgat cgcgaccggg gtggcgcggc gacaggccca
     5101 ggaggcg
//
LOCUS       DAOPQX010000263         5104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17819_length_5104_cov_4.498515, whole genome shotgun sequence.
ACCESSION   DAOPQX010000263 DAOPQX010000000
VERSION     DAOPQX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17819_length_5104_cov_4.498515"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKK95_12810"
     CDS             complement(<1..591)
                     /locus_tag="PKK95_12810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC19148.1"
                     /translation="MKSRPSTLLGAGGRPALRARALGIMLEGHTRMAQQGGALRTGAR
                     LGPYTVVSRLGSGGMGEVLRARDERLGREVALKVVHPRLSGPEAAARFEREARLLASL
                     AHPHVATIFGFEEADGVRFAVLELVEGQTLRELLAGGPVAVERALRLAIQIADALEAA
                     HARHIAHRDLKPENVKVTPDGRVKVLDFGIAQVIDAN"
     gene            607..>5104
                     /locus_tag="PKK95_12815"
     CDS             607..>5104
                     /locus_tag="PKK95_12815"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOC19149.1"
                     /translation="MPSDGFDGAHESGEGAELLGLERLEEHARRLAALLTPERRGRSG
                     RAEHLRRLREDSRTLRRIYTALEEDARAGEATPPAAEWLLDNFHVIAAAIRDVRSDLP
                     PSFYRRLPSLTADEFAGLPRVSAMARELIRSSAGRLDAHRIQRFMTAFQSVTPLTIGE
                     IWAWPSALKLALVEQLRARAEVIFAARAQRLEADRLVGTLDSSPPEPRAWPDPFHPAF
                     VIRVLQRSREHGAASAALRQQLGAELDARGQTIEDVIRAEARYQAASQAAMANLVESL
                     RLVSTFDWSEFFESVSLVEQVLLRDPSAVYGRMDFRSRDRYRHAVEELAEPTGEGQVR
                     VALKAVERARQVAERDREARGAHVGYYLIGGGRAKFEQGIGWTPGPRQRLRRLAFRYA
                     TPLYLGAITLVTVAILAALAAYSLRHDPGTVGLAIVMLLALVPASELAVQIVQRIITR
                     LIPPRRLPRLDLLQVPDSACTMVIVPTLLDSVDGVRELLSHLEVQALGNVDPNVHFAI
                     LSDFRDAPTESLPRDAELLEAARSGIEALNARYAPETNGRFFLFHRARQWNPQENIWM
                     GWERKRGKIEEFNRLLRGATDTSFVLHVGNLAVLPKVRYCITLDSDTRLPRGAARELI
                     GIISHPLNRPRFDPKARRVVDGYGILQPRVSVTFASAEGSLFARLFAGHTGVDPYTTA
                     VSDTYQDLFGEGIFTGKGLYDVDAFTSALEDVVPENVLLSHDLFEGLHARVALVSDVE
                     VVDDYPSTVLSHARRQHRWVRGDWQILLWLFPFVPSRRGFVRNTLPLIGRWKILDNLR
                     RSLVAPMLMALLVAGWTVLPGSHWLWSGVVFAVVAAQLLPLVARLVTGPRRAQSIPVF
                     LRNLRNDAAVALAQAGLGIAFLAYHAFLTAHAIALALVRLAFTRKRLLEWETAAAVAA
                     RTAGLTGRQALGHYHAEMASSPIAAVASAFLVAAVNPSALPAASPFLALWLLAPGIGY
                     WISLPAAARVQPLSAAERLALRRAARKTWRFFEAFVTEEDAWLPPDNFQEDGAGPRLA
                     RRTSPTNIGLGLLSTLAAHDLGYITASELADRIDRTLTTLEGLEQHEGHFLNWYDTAT
                     LAPLYPRYVSTVDSGNLAAALIALAQGLRQAADEPQTPAQRLAGLADTADLLAEASRL
                     QGNVNQLARAIAAAARAGTPPADLAQLRALAADLAAAWPAAAPADPREPESDLTFWVR
                     AVVDAVERLDGRASSTGVPPVSETGVSPVSATGVPPVPATGVPPVSATPAGPSLHPAE
                     HLHALAARAARLADDMRFDFLYDRRRRIFAIGYRLADAEGPGRLDTSFYDLLASEARL
                     ASFVAIAKGDVPQHHWFHLGRMVTNVNGRAVLVSWGGTMFEYLMPLLLMRGFRGTLLD
                     RSCRASVWRQIEYARQHGVPWGISESAYSLTDRDGTYQYKAFGVPGLGLKRGLSDELV
                     VSPYSTALASLVNAPAATANLEVLRRVGLDGRFGFYEAVDYRPRAKVDPDAPDVPAKA
                     SPVVV"
BASE COUNT          656 a         1947 c         1758 g          743 t
ORIGIN      
        1 gttggcgtcg atcacctgcg cgataccgaa gtcgagcacc ttgacgcggc cgtcgggcgt
       61 caccttgacg ttctctggct tcagatcgcg gtgggcgatg tgcctggcgt gcgccgcctc
      121 gagggcgtcg gcgatctgga tggccagccg cagggcccgc tcgacggcga cggggccacc
      181 cgccagcagc tcgcggagcg tctgtccctc taccaactcg agcaccgcga agcggacgcc
      241 gtcggcctcc tcgaacccga agatcgtggc cacgtgcggg tgggcgagcg aagcgagcag
      301 cctggcctcg cgctcgaagc gcgcggcggc ctcgggcccg ctcagccgcg ggtgaacgac
      361 cttgagcgcc acttcgcggc cgagccgctc gtcgcgcgcc cgcagcacct cgcccattcc
      421 acccgagccc aggcgcgaga cgacggtgta cggcccgagc cgcgcgcccg tgcgcagcgc
      481 tccgccctgc tgtgccatgc gtgtgtgccc ttcgagcatg ataccaagcg cgcgcgcccg
      541 tagcgccggc ctcccaccgg ccccgagcag cgtcgagggg cggctcttca tgagagaatt
      601 gtcaaaatgc catctgacgg gttcgatggc gcccacgaat cgggcgaggg cgccgaactg
      661 ctcggcctcg agcggctcga ggagcatgcg cggcggctgg cggcactgct gacgcccgag
      721 cgccgcggcc ggtcgggccg cgccgaacac ttgaggcggc tcagggaaga ttcgcggacg
      781 cttcgccgga tctacaccgc cctcgaggag gatgcgcgcg ccggcgaggc gacgccgccc
      841 gccgccgagt ggctgctcga caacttccac gtgatcgcgg ccgccatcag ggacgtccgc
      901 agcgacctcc cgccctcgtt ctaccgccgc ctgccgtcac tcaccgccga cgagttcgcc
      961 ggcctgccgc gcgtgtcggc catggcgcgc gagctgatcc gttcgagcgc cggccgcctc
     1021 gacgcgcacc gcatccagcg cttcatgacc gcgttccagt cggtcacgcc gctgacgatt
     1081 ggcgagatct gggcgtggcc gagcgcgctg aagctcgcgc tggtcgagca gctgcgcgcc
     1141 cgggccgagg tcatcttcgc ggcccgtgct cagcgcctcg aggccgaccg gctggtggga
     1201 accctcgact cctccccgcc ggagccgcgt gcctggcccg accccttcca cccggccttc
     1261 gtcatccgcg tgctgcagcg atcgcgcgag cacggggcgg cgtccgccgc gctccgccag
     1321 cagctggggg ccgagctgga cgcgcgcggg cagaccatcg aggacgtgat ccgcgccgag
     1381 gcgcggtacc aggcggccag ccaggccgcg atggccaacc tcgtcgagag cctgcgcctg
     1441 gtctccacct tcgactggag cgagttcttc gagagcgtca gcctggtcga gcaggtgctg
     1501 cttcgcgatc cgtcggccgt gtacggacgg atggacttcc gcagccgcga ccgctaccgg
     1561 cacgcggtgg aggagctggc cgagccgacc ggcgaggggc aggtgcgggt ggcgctcaag
     1621 gccgtcgaac gggcgcgcca ggtggccgag cgcgatcgcg aggcgcgcgg ggcccacgtc
     1681 ggctactacc tcatcggggg cggccgcgcg aagttcgagc aggggatcgg ctggacgcca
     1741 ggcccgcggc agcgccttcg ccgcctcgcg ttccgctacg ccaccccgct ctacctcggc
     1801 gcgatcacgc tcgtcacggt ggcgattctc gcggcgcttg ccgcttactc cctgcggcac
     1861 gacccgggca ccgtgggcct ggcgatcgtc atgctgctcg cgctcgtccc ggcgagcgag
     1921 ctggcggtgc agatcgtgca gcggatcatc acgcggctga tccccccgcg gcggctcccg
     1981 cgcctcgacc tgctgcaggt gcccgactcg gcgtgcacga tggtgatcgt gcccacgctg
     2041 ctcgacagcg tggacggcgt gcgcgagctg ctgtcgcacc tcgaggtgca ggcgctcggc
     2101 aacgtcgatc ccaacgtcca cttcgccatc ctcagcgact tccgcgacgc ccccaccgag
     2161 agcctgcccc gcgacgccga gctgctcgag gcggcgcgct cggggatcga ggcgctgaac
     2221 gcgcggtacg cccccgagac caacggccgg ttcttcctgt tccaccgggc gcgccagtgg
     2281 aacccgcaag agaacatttg gatgggctgg gagcgcaagc gggggaaaat cgaggagttc
     2341 aaccggctcc tgcggggcgc caccgacacg agcttcgtgc tgcacgtcgg caacctcgcc
     2401 gtgctgccga aggtccgcta ctgcatcacg ctcgactcgg acacgcgcct gccgcgcggc
     2461 gccgcccgcg agctgatcgg catcatcagc cacccgctga accgcccccg gttcgacccg
     2521 aaggctcgcc gggtggtgga cggctacggc atcctgcagc cgcgcgtcag cgtcactttc
     2581 gcgagcgccg aggggtcgct cttcgcccgg ctgttcgccg ggcacaccgg cgtcgatccc
     2641 tacacgacag cggtttccga cacctaccag gacctgttcg gcgaaggcat cttcaccggc
     2701 aaggggctgt acgacgtgga cgcgttcacc tcggcgctcg aggacgtcgt ccccgagaac
     2761 gtgctcctgt cgcacgacct gttcgagggg ctgcacgcgc gcgtggcgct ggtgtcggac
     2821 gtcgaggtgg tggacgacta cccgtccacc gtgctttccc acgcccggcg ccagcaccgc
     2881 tgggtgcgcg gcgactggca gatcctcctc tggctgttcc ccttcgtccc ctcccgccgc
     2941 ggcttcgtcc gcaacacgct gccgctcatc ggccgctgga agatcctcga caacctgcgg
     3001 cgcagcctcg tcgcgccgat gctgatggcg ctgctggtcg ccggctggac ggtgctgccc
     3061 ggcagccact ggctctggag cggcgtcgtc ttcgcggtgg tggcggcgca gctgctgccg
     3121 ctcgtcgccc gcctcgtcac cgggccgcgc cgcgcgcagt cgatcccggt gttcctccgc
     3181 aacctgcgta acgacgccgc cgtcgcgctg gcgcaggccg ggctcggcat cgccttcctc
     3241 gcctaccacg cgttcctcac cgcccacgcg atcgcgctgg cgctggtgcg cctggccttc
     3301 acccggaagc ggctcctcga gtgggagacc gcggcggcgg tcgccgcgcg aaccgccggc
     3361 ctcaccggca gacaggcgct cggccactac cacgccgaga tggcgtcgag cccgattgcc
     3421 gcggtggcct cggccttcct tgtggccgcc gtcaacccgt ccgcgctgcc cgcggcctcg
     3481 ccgttcctgg cgctctggct gctcgcgccg gggatcgggt actggatcag ccttccggcc
     3541 gccgcacgcg tgcagccgct ctcggccgcc gagcgcctcg cgctgcggcg cgcggcgcgc
     3601 aagacctggc ggttcttcga ggcgttcgtc accgaagagg acgcctggct gccgcccgac
     3661 aacttccagg aggacggcgc cgggccgagg ctggcgaggc gcacctcgcc caccaacatc
     3721 ggcctgggcc tgctctcgac gctcgccgcc cacgacctcg gctacatcac ggcttccgag
     3781 ctggccgacc gcatcgatcg cacgctgacc acgctcgagg ggctcgagca gcacgagggc
     3841 cacttcctca actggtacga caccgccacg ctcgcgccgc tctatccccg ctacgtctcg
     3901 acggtggaca gcgggaacct cgccgccgcg ctgatcgcgc tcgcgcaggg cctccggcag
     3961 gccgccgacg agccgcagac gccggcccag cgccttgccg gcctggccga cacggccgac
     4021 ctgctggccg aggcgtcgcg cctgcagggg aacgtgaacc agttggcccg cgcgatcgcc
     4081 gcggccgccc gagcggggac gccacccgcc gacctcgccc agctgcgcgc gttggccgcg
     4141 gacctggccg cggcatggcc tgcggccgcg ccggccgatc cccgcgagcc ggagagcgat
     4201 ctgaccttct gggtgcgcgc cgtggtcgac gccgtcgagc gcctcgacgg gagggccagt
     4261 agcacaggcg tcccgcctgt gtcagagaca ggcgtctcgc ctgtgtcggc gacaggcgtc
     4321 ccgcctgtgc cagcgacagg cgtcccgcct gtgtcagcga cgccggctgg gccttcgttg
     4381 caccccgccg aacacctgca cgccctggcc gcgcgcgccg cgcgcctggc cgacgacatg
     4441 cggttcgact tcctctacga ccgccggcgg cgcatcttcg cgatcggcta ccgcctggcc
     4501 gatgccgagg gacccggaag gctcgacacg tcgttctacg acctgctcgc ctccgaggcg
     4561 cggctcgcga gcttcgtcgc catcgccaag ggcgacgtgc cgcagcacca ctggttccac
     4621 ctcgggcgga tggtgaccaa cgtcaacggg cgcgcggtgc tcgtgtcgtg gggcggcacc
     4681 atgttcgagt acctgatgcc gctgctgctg atgcgtggct tcaggggcac gctgctcgat
     4741 cggtcctgcc gcgccagcgt gtggaggcag atcgagtacg cccggcagca cggtgtcccg
     4801 tgggggatct ccgagtcggc gtactcactc accgaccgcg acgggacgta tcagtacaag
     4861 gccttcggcg tcccgggcct cggcctcaag cgcggcctct ccgacgagct ggtggtgtcg
     4921 ccctattcca ccgcgcttgc cagcctcgtg aacgcgccgg cggccaccgc gaacctcgag
     4981 gtgctccgcc gggtcggtct cgacggccgc ttcggcttct acgaggcggt ggactaccgg
     5041 ccgcgggcga aggtcgaccc cgacgccccc gacgtgccgg ccaaggcgtc gcccgtcgtc
     5101 gtcc
//
LOCUS       DAOPQX010000264         5086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17899_length_5086_cov_4.077718, whole genome shotgun sequence.
ACCESSION   DAOPQX010000264 DAOPQX010000000
VERSION     DAOPQX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17899_length_5086_cov_4.077718"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..968
                     /locus_tag="PKK95_12820"
     CDS             303..968
                     /locus_tag="PKK95_12820"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOC19150.1"
                     /translation="MLFGSTRRRVLAWLLGHPDEAYYLRQIARQTGAAVGAVQRELKL
                     LTAAGLVRRTVQGRQVYFQADREAPIFPELRGLFAKTAGLVDILRDALAPLGDRIQVA
                     FVFGSAARNELRAASDIDLRVVGDAPFLDVVQAIAAAKEQLGRDINPTVYPPHEFEAK
                     LATHHHFLTTVLQGPRLFVVGGNDELAGLGAERLVEEASEQPKRDSRTARGRRARPRR
                     QRR"
     gene            991..1302
                     /locus_tag="PKK95_12825"
     CDS             991..1302
                     /locus_tag="PKK95_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19151.1"
                     /translation="MNIAYNAALQAATAALAAAGYRASRESHHYRVIQSLRETLGTEA
                     AVVATFDAFRKKRNIVGYERIGLVSDADADAMRALAIQLRDQVVAWLRTHHRHLLPPT
                     S"
     gene            complement(1543..1845)
                     /locus_tag="PKK95_12830"
     CDS             complement(1543..1845)
                     /locus_tag="PKK95_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19152.1"
                     /translation="MTSRVVRPPGSRCATPGANAGSIPSRVVASDQDWRIAVRGDRAH
                     RPLDGAHGIVTPGDGDVARVEQPIGARHVHEGLGPRVRRVRMEGLADQRRRFRRPA"
     gene            1826..1990
                     /locus_tag="PKK95_12835"
     CDS             1826..1990
                     /locus_tag="PKK95_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOC19153.1"
                     /translation="MTTREVITLLHGLEGPIIGADIVEFNPRQDPHGLTAWVAAKLVK
                     EIAGLVLRQG"
     gene            complement(2196..3842)
                     /locus_tag="PKK95_12840"
     CDS             complement(2196..3842)
                     /locus_tag="PKK95_12840"
                     /inference="COORDINATES: protein motif:HMM:NF016345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HOC19154.1"
                     /translation="MPRALLSLVVWALVLAAAGLAAPVRAVPQRDLCLFETPHFDVYY
                     SPAEREAARQVAVLAERSYTELSRLFERSLARRHSIVLYPTGSRYRAEVTAGGMLSAG
                     SGGVTRGEHAAIAMPIGASLAETEHVVAHELVHAFQYEAASRSNPPFPAVPPWFVEGM
                     AECLALGADHPGVLAWARDASRRARGPALAELSDPKHLPYLAGHGAWRFLFARYGRDV
                     GRRLLAAPGNSLEARLHAVTGLTLGELADSWRRSLRAEFGGGHGRIDGRVVARDAVGP
                     SLSPDGRLLAWASPGEPARLWLSDAASGVVRRRLLDPATNPRYDSLHALDSAGAWDPS
                     GRRIAFGASRGGQAFIAITNAATGNLEREVGLDEPGEIRGPAWSPDGRRLAFSGLAGG
                     RTDLYVYDLEAGTLRRLTSDAYAELQPAWSPDGATLVFASDRFTTDLDALRPGLLRLA
                     AIDVATSTIRELPGTSGGRNFSPRFGRGDELFFVADPDGSPSVYRVPLSGGEASRVTP
                     PGVEVTGPTPSGPVLSATRGGALAFSFFRDGRYQVALVRP"
     gene            4073..>5086
                     /locus_tag="PKK95_12845"
     CDS             4073..>5086
                     /locus_tag="PKK95_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC19155.1"
                     /translation="MPPRAPRRRVQPKVSRRAAALGAGAAAALTLAIVVGLAVFRPSP
                     RAAAPIVVISIDTLRADRVGAYGYPAAKTPEIDRLAREGVLFERAFSHSPQTLPSHAS
                     MLTGLLPFEHGVRDNVGFKFGTEAGAAVTLPQVLGQRGYRTAGFVSAFVLRPETGIGA
                     GFEHFDADLPPGAPGASMGSVRRDGADTVERARAWLAGQPDGRFFLFVHLYEPHRSWR
                     VPRGFDAADRYDAAISYADSLVSRLLGALAEKGFYDDGLVVVLSDHGEGLGDHGEEEH
                     GVFLYDESVRVPFVVKMPGGRHGGARVREPIGLVDLFPTLAEVAGTAVDRALEGRSLL
                     PLLA"
BASE COUNT          771 a         1794 c         1782 g          739 t
ORIGIN      
        1 acgcggggat gcaccctcgc cgaggacccc gtttccacgc cgtctcggag gttcccccgc
       61 gcacgcgggg atgcaccccg ctcgcacggg cacttgcagc aggacaacgc ggttcccccg
      121 cgcacgcggg gatgcgaagt cccgaagtca cgaagtcgcg aagtcgagtc cacgaagtcg
      181 cgagtcgtag gggcggccct tgtggccgcc gcttcccttg gcccttgacg tatgttcgcg
      241 ttgtgagtac gtttgttccc atcgtgagaa caatctcgca agcgccatcg gaccccacaa
      301 cggtgctctt cggcagcacc cgccgtcggg ttctggcttg gttgctcggt caccctgacg
      361 aggcgtacta ccttcggcag attgcccgtc agacgggtgc tgcagtcggc gccgtgcagc
      421 gggaactgaa gctgctgacg gctgcagggt tagtgcgtcg aaccgttcaa ggacgccagg
      481 tttacttcca agccgatcgc gaagcaccga ttttcccaga actgcgtgga ttgtttgcga
      541 aaactgcggg actcgtcgac atcttgcgcg acgcacttgc gccgctgggc gacaggatcc
      601 aggtcgcctt cgtgttcgga tcggcagcac gtaacgagct ccgtgcggca agcgacatcg
      661 accttcgcgt agttggagac gcgccattcc tggatgtcgt gcaggctatc gcggcggcca
      721 aggaacagct cggcagggac atcaacccaa cggtctatcc gccgcacgag tttgaagcca
      781 agctcgccac ccaccaccac ttcctgacga ccgttctgca aggcccgcgg ctgttcgtcg
      841 ttggagggaa tgatgagctt gccggactgg gagcggaacg gctggttgaa gaagcatcag
      901 agcagcccaa acgagattcg cgaactgctc gaggtcgtcg agcgcgacct cgccgacagc
      961 gccgctgaag atctgagtgc ggactgacgc atgaacatcg cgtacaacgc cgcgctgcag
     1021 gcggcgacag ccgcgctggc agccgctgga taccgtgcgt cgcgagaatc gcatcactac
     1081 cgcgtgatcc aatccctccg cgagacgctc ggtaccgagg cagccgttgt cgcgaccttc
     1141 gacgcgttca ggaagaagcg gaacatcgtc ggctacgaga ggatcggact cgtgtccgac
     1201 gcagacgcag acgcgatgcg cgcgctcgcc attcagcttc gcgaccaggt cgtcgcgtgg
     1261 ctacgtacgc accaccgcca cctgcttccg ccgaccagct aatcctcagc ttccctcccg
     1321 agcgtaccta tcgcccttgc cgccggcgcc gtctaggagc ctcgaggttg acagggccaa
     1381 gtccccggtc gcacatcggc ggccgtacca agaagtcgcg cgaagtcgca cggcgtccgc
     1441 aggtggctct ggtagcattc ccccgtgagc cggacgacat cgaccagccg acgcccgcgc
     1501 ccggtgctcc tcggcgtccc ctacgacttg gcgtcgtcgt ttctacgcgg ggcggcggaa
     1561 gcgccgccgc tggtccgcca ggccctccat tcggacgcga cgaacacgtg gtccgagtcc
     1621 ctcgtggacg tggcgggcac cgatgggctg ctcgacgcgg gcgacgtcgc catcgccggg
     1681 cgtgacgatg ccgtgcgcgc catcgagggg gcggtgggcc cgatcgccgc gaacggcgat
     1741 acgccaatcc tgatcggagg cgaccactct cgacgggatc gaccccgcgt tcgccccggg
     1801 cgtcgcacac cgcgagcccg gcggcctgac cacccgagag gtcatcaccc tccttcacgg
     1861 cttggaaggc cccatcatcg gcgccgacat cgtggagttc aacccgcgac aagatccaca
     1921 cggcttgacc gcctgggtcg cggcgaagct ggtcaaggag atcgcgggcc tggtgctgcg
     1981 acaaggctga agccgccacg acgcgcgcta cggcggaatg cggctaaagt cccgtaccac
     2041 gcctgtcggg tttcacaggc gggacgcctg tgccactgta gccaggttcc acaggcggga
     2101 cgcctgggcc actgtagcca ggtttcacag gcgggacgcc tgtgccactg tagccagatt
     2161 cacactcggg acgcctgtgc cactgaaagc ccggttcacg gacgcacgag cgcaacctgg
     2221 tagcggccgt ctctgaagaa gctgaacgcg agggcgccgc cgcgcgtggc cgagagcacg
     2281 gggcccgagg gggtgggacc ggtcacctcg acgccgggcg gcgtcacccg cgaggcttca
     2341 ccacccgaga gcggcacgcg gtagacgctc ggcgacccgt cggggtcggc cacgaagaac
     2401 agctcgtcgc cgcgcccgaa gcgcgggctg aagttgcgcc cgccggacgt gcccggtagc
     2461 tcgcggatcg tcgaggtggc cacgtcgatg gcggcgagcc gcaggaggcc cggccgcagc
     2521 gcgtcgaggt cggtggtgaa gcggtccgag gcgaagacga gggtggcgcc gtcgggcgac
     2581 cacgccggct gcagctcggc atacgcgtcg ctggtgagcc ggcgcagcgt gcccgcttcc
     2641 aggtcgtaga cgtagaggtc ggtccggccg ccggccagtc cggaaaatgc gaggcggcgg
     2701 ccgtcgggcg accaggccgg cccgcgaatc tcgcccggct cgtcgaggcc aacttcgcgc
     2761 tcgaggttcc cggtcgcagc gttggtgatc gcgatgaatg cctgcccccc gcgagacgcg
     2821 ccgaaggcaa tgcgccgtcc cgacggatcc cacgctcccg cgctgtcgag cgcgtgcagg
     2881 ctgtcgtagc gcgggttggt cgcgggatcc agcagccggc gccggaccac gcccgacgcg
     2941 gcgtcggaga gccaaagccg ggccggctcg cccggcgacg cccacgccag cagccggccg
     3001 tcggggctca gcgaggggcc gaccgcatcg cgcgcgacca cccggccgtc gatccggccg
     3061 tgcccgccgc cgaactcggc tcgaagcgat cgcctccagc tgtcagccag ctcgcccagg
     3121 gtgagtccgg tcacggcgtg cagccgcgct tcgagggaat tcccgggagc ggcgagcagc
     3181 cggcgtccga cgtcccggcc gtagcgcgcg aagaggaaac gccaggcgcc gtgcccggcg
     3241 agatacggca ggtgcttcgg atcggacagc tccgcgagcg ccgggccgcg cgcccgcctc
     3301 gacgcgtccc gcgcccacgc aagcacgccg ggatgatcgg cgccgagcgc caggcactcg
     3361 gccattccct cgacgaacca gggcggcacc gccggaaaag gcggattgga gcgagaggcg
     3421 gcctcgtact ggaacgcgtg caccagctcg tgtgccacga cgtgttccgt ctccgcaagg
     3481 ctcgcgccga tcggcatcgc gatggccgcg tgctcgccgc gggtgacgcc gccgcttccc
     3541 gcgctcaaca tcccgcccgc cgttacctcg gcgcgatagc gcgagccggt cgggtagagc
     3601 acgatcgagt ggcggcgggc gaggcttcgc tcgaagaggc gcgacagctc ggtgtaggaa
     3661 cgctcggcga gcactgccac ctggcgggcg gcctcgcgct cggcgggtga gtagtagacg
     3721 tcgaagtggg gcgtctcgaa caggcacagg tcgcgctgcg ggacggcgcg gacgggcgcc
     3781 gcgaggccgg ccgccgccag cacgagggcc cagacgacga gcgagagcaa ggctcgcggc
     3841 atgggttcag cgaggacgtc cagttgtgct gcgggggcgg gcgcggagaa ctgacaaggt
     3901 atcccgcccg gcgctcagcg gcaaggtcag ccgttgacgc gccgctcctt cgacgccgcg
     3961 tctcgtcccg gcccgcccgc cgctcgtccc agtctgtccg ccgcccgtcc ccggggagtt
     4021 gccgttcggc ccggccgcgc cgatcctgcc atgagaggct cagaaggcca ccatgccccc
     4081 acgtgcgccc cgcaggcggg tccagccgaa ggtgtcgcgc cgcgccgccg cgcttggagc
     4141 cggcgctgcc gccgcgctga cgctcgccat cgtcgtcggg ctcgcggttt tccgcccctc
     4201 gccgcgcgcc gccgccccca tcgtcgtcat ctcgatcgac acgctgcgcg ccgaccgcgt
     4261 cggcgcctat gggtaccctg ccgcgaagac gccggagatc gatcggctgg cgcgggaagg
     4321 ggtcctcttc gagcgggcct tctcacactc gccgcagacg ctcccgtcgc acgcgtcgat
     4381 gctcacaggg ctgctgccct tcgagcacgg ggtacgggac aacgttggat tcaagttcgg
     4441 tacggaggcc ggcgctgctg tgacgctgcc ccaggtgctc ggccagcgcg gataccgcac
     4501 ggccgggttc gtctcggcgt tcgtcctgcg gcccgagacc ggcatcgggg ccggctttga
     4561 gcacttcgac gccgacctgc cgcccggcgc gcccggcgcg tcgatgggaa gcgtgcgccg
     4621 cgacggcgcc gacacggtgg agcgggctcg cgcctggctg gcggggcagc ccgacggccg
     4681 cttcttcctg ttcgtccacc tctacgagcc tcaccgttcc tggcgcgtcc ctcgcggctt
     4741 cgacgcggcc gaccggtacg acgcggccat ctcgtacgcc gactcgctgg tgagccgcct
     4801 gctcggcgcg ctggccgaga aggggttcta cgacgatggg ctggtcgtcg tgctctcgga
     4861 tcacggcgag gggcttggcg accacggcga agaggagcac ggcgtctttc tctacgacga
     4921 atcggtcagg gtgcccttcg tcgtgaagat gccgggcgga cggcacggcg gtgcgagggt
     4981 gcgcgagccg atcggcctgg tggacctctt cccgacgctg gccgaggttg ccggcaccgc
     5041 cgtggatcgc gcgctcgagg ggcgctcgct gctgccgctg ctggcc
//
LOCUS       DAOPQX010000265         5071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17969_length_5071_cov_4.308812, whole genome shotgun sequence.
ACCESSION   DAOPQX010000265 DAOPQX010000000
VERSION     DAOPQX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17969_length_5071_cov_4.308812"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKK95_12850"
     CDS             complement(<1..551)
                     /locus_tag="PKK95_12850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOC19156.1"
                     /translation="MGEVRIEHLGDDRLEEAAATLARAFVTNPIHVAAFGAGQLAVNE
                     TFFRLGLPLMRGTRLVALEDGALVGLIHWIESPRCQPTDQDRRTLAPLMLKTFGIRTA
                     WRLRSWLTAWSGHDPQGPHVHLGPIGVEPSAQGRGIGRLLMRRYCEQLDASRQVGYLE
                     TDRPENVPFYEGFGFRVTGEMLVL"
     gene            672..1526
                     /locus_tag="PKK95_12855"
     CDS             672..1526
                     /locus_tag="PKK95_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC19157.1"
                     /translation="MPTDPTRRDVLKLGGALAALASVSPSLRASARAAWKQVPIGTQL
                     WCVRKQLATDIPGTLGALAAAGFDGVELENAFGKPGAEWRKHLDAAGLKPCGFHHTLE
                     ELGGDKLEATVEFNQAIGNRYLIVRSLREEIYKSEDLLKKAADAVNVAAQQVKRHGLL
                     VGYHNHALDFHRLGGEYWWNLFADRTTKDVILQLDTGNASEAIDLVGLVRRNAGRTVT
                     MHVKPFSKKDPDAYIGADELDWPALMTAAETVGGVEWYIVEYEREGVPPIEALKSNLD
                     ALKKLRAG"
     gene            1588..2556
                     /locus_tag="PKK95_12860"
     CDS             1588..2556
                     /locus_tag="PKK95_12860"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOC19158.1"
                     /translation="MPRTLGEQGGQHRPAPDGGLGVEALRAPAGLHRFSHEAMATIFE
                     VHTAHPDAQYAAQAAQAVFDLVDRLERELSRFLANSDVARINGLAAGASTRVTPTTLE
                     CLVIARHLYDLTGGAFDVSIGTGLPLLELDPDGLVVRAREGGVRIDLGGIGKGYAVDL
                     AAELLEEWDLGASLVHGGFSSVLVLEPPPALEGWALTLSDPAAPARLLARLSARQAAL
                     SASGLRKGDHIRDPRTGEAVRGRRAAWTLVPRPETAKADRAPREGPRMAAAAVADALT
                     TAFMLLGLEEIESICAASPGLEAWVLPEAGADGSADLLHFAAPVEP"
     gene            2741..4177
                     /locus_tag="PKK95_12865"
     CDS             2741..4177
                     /locus_tag="PKK95_12865"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC19159.1"
                     /translation="MAGEEDKNKVMPPIAAGGPRKLDRRDVLKGLSTVPALGLFAYAW
                     QQQRQYDQATLQATAAPAAAGDVQELNIALIGAGAQGQVLTDAMLRIPGLRFRAVCDV
                     WTEYNLRRVVNILRRFKHEPNAYEDYREMLDKEKELDAVVIATPDFWHAPHTVECLKA
                     GKHVYCEKEMSNTLEGARSMVAAARETGKLLQIGHQRRSNPRYLHCYEKLLNEAKLLG
                     RIVTINGQWNRAVAADLGAPDRYAIPEERLKQYGFKNMHQFRNWRWYKGLGGGPIVDL
                     GSHQIDIYTWFLGTNPSAVMASGGLLYYDPATHEWYDTVMVVYDYQTPQGPVKAYYQT
                     QTTNGSQGYFENFMGDQGTLLISESELNYAGKIYRDPNAPAWDEWIQKGYISAPKLQE
                     VQSQEKTGAIADVRESVSPDEHTVPVVLRDPYHQPHLQNFFDAVRGKAKLNCPPEVGY
                     ESAVSVLKVNEAIDAKSILAFKPEDFHI"
     gene            4191..4706
                     /locus_tag="PKK95_12870"
     CDS             4191..4706
                     /locus_tag="PKK95_12870"
                     /inference="COORDINATES: protein motif:HMM:NF016089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOC19160.1"
                     /translation="MEGATFSRSAMIAITVMRVLVGWHFMYEGIAKLTSSSWSAAGYL
                     RQARGPAAGFFRWLAAQPNLLAYADQITMWGLTIVGALLILGLFTRLSALAGIGFLVL
                     FYAANPPFVGYFYSLPSEGSYLIVNKQLVELGALLVIMVTGSGKYAGLDRIVHALITR
                     KPAPAVVSPAR"
     gene            4868..>5071
                     /locus_tag="PKK95_12875"
     CDS             4868..>5071
                     /locus_tag="PKK95_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19161.1"
                     /translation="MDNPFVYGEVVPDAAFVDREVELDRLVRDLLAGQKVFLISPRRY
                     GKSSLVRQALASAGRAGALAVSVT"
BASE COUNT          809 a         1764 c         1771 g          727 t
ORIGIN      
        1 agcaccagca tctctcccgt cacgcggaaa ccgaagccct cgtagaacgg aacgttctcg
       61 ggccgatccg tctcgagata tcccacctgc cgcgaggcgt cgagctgctc gcagtagcgc
      121 cgcatgagca gccggccgat accgcggccc tgcgccgatg gctccacgcc gatggggccg
      181 aggtgcacgt gcgggccctg cgggtcgtgt cccgaccagg cggtgagcca cgagcggagg
      241 cgccacgccg tccggatccc gaacgtcttc aacatcagcg gagcgagcgt ccgcctgtcc
      301 tggtcggtgg gctggcagcg cggcgactcg atccagtgga tcagcccgac gagagcgccg
      361 tcctcgaggg cgacgagcct cgtccccctc atcagcggca gcccgaggcg gaagaacgtc
      421 tcgttcacgg caagctggcc ggccccgaac gccgccacgt ggatcgggtt ggtgacgaac
      481 gcccgggcca gcgtggcggc cgcctcctcg agccggtcgt cgccgaggtg ttcgatccgg
      541 acttcaccca tcggcttctc gggacgtagt gtgacagcct tcgaggcctt tcgcacgcgt
      601 tcgatacggc cgccgcttgc cgcagcggcc gccgcgggcg taggatgcga cgcagccgaa
      661 aggagcgttc catgccgacc gatccgaccc gccgcgacgt cctgaaactg ggcggtgccc
      721 ttgccgccct tgcgtctgtc tcgccgtccc tgcgggcgtc ggcccgggcg gcctggaagc
      781 aggtgccgat cggcacgcag ctctggtgcg tgcgcaagca gctcgccacc gacattcccg
      841 gcacgctcgg cgcgctggct gccgccggat tcgacggcgt cgaactcgag aacgccttcg
      901 gaaagccggg tgcggagtgg cggaagcacc tcgacgcggc cggcctgaag ccatgcggct
      961 tccaccacac gctcgaggag ctgggcggcg acaagctcga ggccaccgtc gagttcaacc
     1021 aggcgatcgg caatcgctac ctcatcgtcc gatcgctccg cgaggagatc tacaagtcgg
     1081 aggatctgct caagaaggcg gccgacgccg tcaacgtggc cgcacagcag gtgaagcggc
     1141 acggcctgct cgtggggtat cacaaccacg cgctcgactt ccaccggctg ggcggcgagt
     1201 actggtggaa cctgttcgcc gaccggacga ccaaggacgt catcctgcag ctcgacaccg
     1261 gcaacgcgtc cgaggccatc gacctggtgg gcctcgtccg gcgcaatgcc ggccgcacgg
     1321 tgacgatgca cgtgaagccg ttttcgaaga aggaccccga cgcctacatc ggcgccgacg
     1381 agctggactg gccggccctg atgacggccg ccgagacggt cggcggcgtc gagtggtaca
     1441 tcgtcgagta cgagcgggag ggggtgccgc ccatcgaggc cctgaaatcg aatctcgatg
     1501 ccctcaagaa gctcagggcc gggtgacccg gaggtgccga agcgggatga ctggattcgg
     1561 cgccggtgat cggggacact ggcgggcgtg cctcggactc tcggcgaaca gggtggtcag
     1621 caccggccgg cgcccgacgg gggcctcggc gtcgaagccc tacgcgcgcc cgcgggtctc
     1681 caccgcttct cgcacgaggc gatggcgaca atcttcgagg tgcacaccgc ccatcccgac
     1741 gcccagtacg cggcccaggc ggcgcaggcg gtcttcgacc tcgtcgatcg gctggaacgc
     1801 gagctgagcc gcttcctcgc caacagtgac gtcgcccgca tcaacgggct cgccgcgggc
     1861 gcgagcacgc gggtcacgcc gaccacgctc gaatgcctgg tcatcgcgcg gcacctgtac
     1921 gacctgacag ggggcgcgtt cgacgtgtcg atcggcacgg ggcttccgct gctcgagctc
     1981 gacccggacg gcctggtggt ccgcgcgcga gagggcggcg tccgcatcga cctcggtggg
     2041 atcggcaagg gatacgcggt ggacctggcg gccgagctgc tggaggagtg ggacctcggc
     2101 gcgtcgctgg tgcacggcgg attcagctcg gttctcgtcc tcgaaccgcc gcccgcgctc
     2161 gagggctggg cgctgaccct cagcgacccg gcggcacccg ctcgccttct cgcgcgcctt
     2221 tccgcccggc aagccgcgct cagcgcctcg gggcttagaa aaggggacca catccgcgat
     2281 ccgcggaccg gggaggcggt gcgcgggcgg cgcgcagcgt ggacgctggt gccgcggccc
     2341 gagaccgcga aggccgaccg ggcgccgcgc gaggggccca ggatggcggc ggccgccgtt
     2401 gccgacgcgc tgaccacggc cttcatgctc ctcggcctcg aggagatcga gtcgatctgc
     2461 gcggccagcc ccggcctcga agcgtgggtc ctgcccgagg cgggcgccga cggttcggcc
     2521 gatctgctgc acttcgccgc gcccgtcgaa ccctgagcga cctgccggcc ggcgcgaggc
     2581 cgcctcgcgc acgggcgagg acgccgtccg cacgggcgag gacgccgtcc gcacaggcga
     2641 ggacgccgtc cgcacaggcg aggacgcctg tgctactggt atactgcacc gatcgcgcag
     2701 gtccttgcgc gctgccccct tccagaagca ggagaacctc atggctggcg aggaagacaa
     2761 gaacaaggtg atgccgccga tcgccgccgg cgggccgcgc aagctcgacc ggcgcgacgt
     2821 gctgaagggg ctgtccaccg tcccggcgct cggcctgttc gcgtacgcct ggcagcagca
     2881 gcgccagtac gaccaggcga cgttgcaggc cacggccgcg ccggccgccg cgggcgacgt
     2941 gcaggagctc aacatcgcgc tcatcggcgc gggagcgcag gggcaggtgc tgaccgacgc
     3001 gatgctgcgc atcccggggc tgcggttccg cgcggtctgc gacgtctgga ccgagtacaa
     3061 cctgcggcgc gtcgtcaaca tcctcaggcg cttcaagcac gagccgaacg cctacgagga
     3121 ctaccgcgag atgctcgaca aggagaagga gctcgacgcg gtggtgatcg ccacgcccga
     3181 cttctggcac gccccgcaca cggtggagtg cctgaaggcc ggcaagcacg tgtactgcga
     3241 gaaggagatg tcgaacacgc tcgagggcgc ccgcagcatg gtggcggcgg cccgcgagac
     3301 cggcaagctc ctgcagatcg gccaccagcg gcggtcgaac ccgcgctacc tgcactgcta
     3361 cgaaaagctg ctgaacgagg cgaagctgct cggccggatc gtgaccatca acgggcagtg
     3421 gaaccgcgcg gtggcggccg atctcggcgc tcccgaccgc tatgcgatcc ccgaggagcg
     3481 gctgaagcag tacggcttca agaacatgca ccagttccgg aactggcgct ggtacaaggg
     3541 gctcggcggc ggtccgatcg tcgacctcgg ctcgcaccag atcgacatct atacctggtt
     3601 cctcggtacg aacccctcgg ccgtgatggc gagcggcggc ctcctctact acgacccggc
     3661 gacacacgag tggtacgaca ccgtcatggt ggtctacgac taccagacgc cgcaggggcc
     3721 ggtgaaggcc tactaccaga cgcagacgac caacggcagc caggggtact tcgagaactt
     3781 catgggggac caggggaccc tgctcatctc cgagtcggag ctgaactacg ccgggaagat
     3841 ctaccgcgac cccaacgccc cggcctggga cgagtggatc cagaagggct acatctcggc
     3901 gcccaagctc caggaggtcc agtcgcagga gaagacgggt gcgattgccg acgtgcgcga
     3961 gtcggtgtcg cccgacgagc acaccgtgcc ggtggtgctc cgcgacccgt accaccagcc
     4021 tcacctgcag aacttcttcg acgccgtccg cggcaaggcg aagctgaact gcccgcccga
     4081 ggtgggctac gagagcgcgg tcagcgtcct gaaggtcaac gaggcgatcg acgcgaagtc
     4141 gatcctggcc ttcaaacccg aggacttcca catctagggg agggtggaac atggaaggag
     4201 caacgttctc ccgctccgcg atgatcgcga tcacggtgat gcgggtcctg gtcgggtggc
     4261 acttcatgta cgaggggatc gcgaagctga cctcgtcctc gtggtccgca gcggggtacc
     4321 tgcggcaggc gcgcgggccg gcggcgggct tcttccggtg gctggccgcc cagccgaacc
     4381 tgctcgccta cgccgaccag atcacgatgt ggggcctcac catcgtcggc gcgctgctga
     4441 tcctcggcct gttcacgcgg ctctcggcgc tggccgggat cggcttcctg gtgctgttct
     4501 acgcggcgaa cccgccgttt gtcggctact tctactcgct cccgagcgag ggcagctacc
     4561 tgatcgtcaa caagcagctg gtggagctgg gcgccctcct cgtgatcatg gtgacgggca
     4621 gcggcaagta cgccggcctc gaccggatcg tgcacgccct catcacgcgg aagccggcgc
     4681 cggccgtggt gtcgccggcc cgctaggccc cgctcgcccg gattgccggc aaggaagggc
     4741 ggtcgcaagg gccgcccttc gtctttggcc cggcattgca cccgccccgc cattacgcta
     4801 gactgcgtaa ccgttacgtc gaactgcgta atgctcccat cgccggggcg gcgctctgga
     4861 gcggctcatg gacaacccgt tcgtgtacgg cgaggtggtg cccgacgcgg cgttcgtgga
     4921 ccgcgaggtg gagctcgacc ggctcgtgcg cgacctgctc gcgggccaga aggtcttcct
     4981 gatctcgccg cgccgctatg gcaagtcgtc gctggtgcgg caggcgctcg cctcggccgg
     5041 gcgcgccggc gccctggccg tctcggtcac c
//
LOCUS       DAOPQX010000266         5069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_17980_length_5069_cov_4.443359, whole genome shotgun sequence.
ACCESSION   DAOPQX010000266 DAOPQX010000000
VERSION     DAOPQX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17980_length_5069_cov_4.443359"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..>828
                     /locus_tag="PKK95_12880"
     CDS             217..>828
                     /locus_tag="PKK95_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19162.1"
                     /translation="MGSKLRRRFARAALAKPAALFLLPLPLLIAIAASLVAGDAYRLA
                     LTGGALTSFWAAGVLATRGIVEEVRHRLGERLLPTAFPWKRLSVLPTALGSGLAATSG
                     GHDPVAALAFALLGGLGHLAFIGTDPSRRRIEVRLMDGVDAAAVERQLEQGHARIRGL
                     LGAGNTFELPEFRERVSRIAHIGEAVLVEIERNPVVAPRARRFL"
     assembly_gap    829..838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <839..1040
                     /locus_tag="PKK95_12885"
     CDS             <839..1040
                     /locus_tag="PKK95_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19163.1"
                     /translation="ARVATEYSRMRARPDNGSLEQNFRRLLVEMEDTFSEQHRLVLER
                     KELLLDADIEVLNTRMSQEGPG"
     gene            1057..2196
                     /locus_tag="PKK95_12890"
     CDS             1057..2196
                     /locus_tag="PKK95_12890"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HOC19164.1"
                     /translation="MTDEQVTTASALAPTAPLPSPEVPRYEGASPEKRAEIEQALTEL
                     HMDDSNAVLFFGTSAQEAVTAVADEMLEGVRNKDTGEAGQALNQLVTTLRGMPIAELD
                     PKKKPGLLRRLSGRARPVARMLQQYEQVRRQVDGISNRLDRHTSALMRDVVMLDRLFE
                     KTLEYFRRLEVYIAAGQEQLRRLDSEVLPALTREAAATQDMVKAQQAADLRARRDDLE
                     RRVHDLKLTRQVVMQSLPSIRLVQQNDKALVGKIASTMANTLPLWRQQLALSLTIARS
                     AEAAETVQKATDLTNELLTANAETLRSSTQAIRTQVERGIVDMAALKQANDALIATVD
                     DALRIADDGKRQRAEAERQLEACEVELRQAVLAARRRGGVEPGRP"
     gene            2284..3657
                     /locus_tag="PKK95_12895"
     CDS             2284..3657
                     /locus_tag="PKK95_12895"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC19165.1"
                     /translation="MILIVDDDPSVTASLALALKQAGRPSHAVSSPSAALSWLEREPC
                     ALVLQDMNFSRRTSGEEGLELLARIRGLRPDLPVILITAWGSIGLAVAGMKAGASDFI
                     TKPWTNEQLLHSVRSVLGVSAARPGEAGTRGPDREELDARYDFGALVGRDPRLLRILE
                     IIGRVAPTDASVLITGESGTGKELVAEAVHRNSRRQRGPFVKVNLGGISSTLFESEMF
                     GHVKGAFTDARSDRKGRFEMADGGTIFLDEIGDLDASSQVKLLRVLQDRTFEVLGSSL
                     RRAVDVRVVTATNRNLGEKVDRGEFREDLLYRINLIAVHLPPLRERRDDIPVLAASFL
                     QTVGAVYQRDGLAMSPAAVRWLQGLPWPGNVRQLRQWIERTVLVSARPVLEVEDFQAT
                     SEMEPPASRDDGLPTVGSMTLDEIEKGMIQKSLRHHGGNISRVAESLGLSRAALYRRL
                     EKYGIET"
     gene            3654..4958
                     /locus_tag="PKK95_12900"
     CDS             3654..4958
                     /locus_tag="PKK95_12900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC19166.1"
                     /translation="MTLSRWLIAYLVVLHLLVAALAFVFLRASPWWLVPIELLLVISL
                     ALGLYLVNRIFGALALVREAQQLLDDGDFMSRVCEVGQPEVDRIIRLFNRLNDRLREE
                     RVQLQEQHHFLGRMLEVSPLGALFLDFDGRVAHANPAAERLLGQGFADLAGRALAEVG
                     GPLAEAAAGLPAGQSTVVSLWGGRRVRCHHGSFLDRGFARSYVLFEELTEELRQFEKA
                     AYGKLIRTMSHEVNNSVGASNSLLHSCLNYAPELPPEHRADFESALSIAIDRTDQLNA
                     FMRSFADVIRLPPPARHPCDLRELVAGVERLVRAQCEERRVAWCWDVEEEPGTVSLDR
                     AQMEQVLLNVVKNALEAVGEDGTIAARFTRREGRPALVIEDSGPGLSADARQNLFTPF
                     FSTKEYGQGIGLTLVQEILGQHRFQYSLDSPPGGPTQFTIVF"
BASE COUNT          753 a         1738 c         1823 g          745 t
ORIGIN      
        1 ggcctgggcc aggggccgga ggccagcggt gcaaccctgc ggcggctatt gccgtacact
       61 taatcatggg tgttgtggca aggcggctgc tcggtctcct tctgctcgtc ctggcgatcg
      121 ggatcccgag ctgccaggca gtgttcccgc gcgcggccgg cgaggagccg cggccggccg
      181 ggcacgttct tccgaggtga cgaggcaaac gtgccgatgg gctcgaagct ccggcgaagg
      241 ttcgccaggg cggcgctggc caagcccgcc gcgctgttcc tgctgccgct gccgctgctg
      301 atcgcgatcg cggcgtcgct cgtggcgggc gatgcatacc gcctggcgct gaccggcggc
      361 gccctgacct ccttctgggc ggcgggcgtc cttgcgacgc gcggcatcgt ggaagaggtc
      421 cgccaccggc tcggcgagcg cctgctgccg acggcatttc cctggaaacg gctgagcgtt
      481 cttcccaccg cgctcggcag cggcctggcc gccacctccg ggggccacga cccggtggcc
      541 gctctcgcat tcgccctgct ggggggattg gggcacctgg ccttcatcgg gacggacccg
      601 agccgccgcc ggatcgaggt gcggctgatg gacggcgtcg acgcggccgc ggtggagcgg
      661 cagctcgagc aggggcacgc acggatccgt gggctcctgg gcgccggcaa cacctttgaa
      721 cttcccgagt ttcgggagcg ggtgagccgc attgcccaca ttggcgaggc ggtgctggtc
      781 gagatcgagc gcaacccggt ggtcgcgccg cgcgcccggc ggttcctcnn nnnnnnnngg
      841 cccgggtcgc gacggagtac agccggatgc gggcccgccc cgacaacggc tctctcgagc
      901 agaacttccg ccggctgctc gtcgagatgg aggacacctt ctccgagcag caccgcctgg
      961 tgctcgaacg caaggagctg ctgctcgacg ccgacatcga ggtgctgaac acccggatga
     1021 gccaggaagg accggggtag ggaagtggag actgacatga ccgatgaaca ggtgacgacg
     1081 gcttccgccc tcgcgccgac cgccccgctg ccctcgcccg aggtgccgcg ctacgagggg
     1141 gcctccccgg aaaagcgcgc cgaaatcgag caggcgctga ccgagcttca catggacgac
     1201 agcaacgccg tcctgttctt cggcaccagc gcgcaggagg cggtgaccgc cgtcgccgac
     1261 gagatgctcg agggggtgcg caacaaggac accggcgagg caggccaggc gctcaaccag
     1321 ctcgtcacca cgctgcgcgg gatgccgatc gcggagctcg atcccaagaa gaagcccggc
     1381 ctgctgcggc ggctctcggg gcgcgcccgg ccggttgcgc ggatgctgca gcagtacgag
     1441 caggtgcggc gccaggtgga cgggatcagc aaccgcctcg acaggcacac gagcgccctg
     1501 atgcgcgacg tggtcatgct cgaccgcctg ttcgagaaga cgctggagta cttccgtcgc
     1561 ctcgaggtgt acatcgcggc ggggcaggag cagcttcgcc gcctcgactc cgaggtgctg
     1621 ccggcgctga cgcgcgaggc ggccgctacg caggacatgg tcaaggcgca gcaggcggcc
     1681 gacctgcgcg cgcggcggga cgacctcgag cggcgcgtgc acgacttgaa gctgacgcgc
     1741 caggtggtca tgcagagcct gccctccatc cggctcgtcc agcagaacga caaggcgctc
     1801 gtcggcaaga tcgcctcgac gatggcgaac accctcccgc tctggcggca gcagctggcg
     1861 ctgtcgctga ccatcgcgcg ctcggccgag gcggccgaga ccgttcagaa ggcgaccgac
     1921 ctgaccaacg agctgctgac ggcaaacgcc gagacgctga ggagctcgac gcaggccatc
     1981 cgcacgcagg tcgaacgggg catcgtcgac atggctgccc tgaagcaggc gaacgacgcg
     2041 ctcatcgcga ccgtcgacga cgcgctgcgg atcgcggacg acgggaaacg ccagcgggcg
     2101 gaggcggaaa ggcagctgga ggcgtgcgag gtcgagctgc gccaggccgt gctggccgcg
     2161 cgccgccgcg gaggcgtcga acccgggcgg ccgtgacctc ggcgacgcgc tctctggcac
     2221 gcagcacgta gcgcagggct tcagccctgc ggctgttcgc tgtccgctgt ctgcttcgtc
     2281 ccaatgatcc tgatcgtcga cgacgacccc tcggtgaccg cttcgctggc gctcgcgttg
     2341 aagcaggccg gccggccgtc gcacgccgtg tcgtcgccct cggcggccct ctcgtggctc
     2401 gagcgcgagc cctgcgcgct ggtgctccag gacatgaact tctcccgccg gacgtcgggc
     2461 gaggagggac tcgagcttct cgcccgcatc aggggtctgc gtcccgatct gccggtcatc
     2521 ctgatcaccg cctgggggtc gatcggtctc gccgtcgccg gcatgaaggc cggtgcctcg
     2581 gacttcatca ccaagccctg gaccaacgag cagctgctgc acagcgtcag gtcggtgctt
     2641 ggcgtgtcgg cggcacggcc cggggaggcg gggacgcggg gcccggatcg cgaggaactg
     2701 gacgcccgct acgatttcgg cgccctggtc ggacgcgacc cgcgcctgtt gcggatcctc
     2761 gagatcatcg gccgcgtggc ccctaccgat gcgtcggtgc tcatcacggg ggagagcggc
     2821 accggcaagg agctggtggc cgaggcggtg caccgcaaca gccgccggca gcgcgggccc
     2881 ttcgtcaagg tgaacctggg aggcatctcc tcgacgctgt tcgagagcga gatgttcggt
     2941 cacgtgaagg gggccttcac cgacgcgcgc agcgaccgga agggccgctt cgagatggcc
     3001 gacggcggga cgatcttcct ggacgagatc ggcgacctcg acgcgtcgtc ccaggtgaag
     3061 ctcctgcgcg tgctgcagga ccgcaccttc gaggtgctcg gatcgagcct gcgccgcgcg
     3121 gtggacgtgc gggtggtgac cgccacgaac cggaacctcg gcgaaaaggt cgaccgcggg
     3181 gagttccgcg aggacctgct gtaccgcatc aacctaatcg cggtgcacct gccgccgctg
     3241 cgcgagcggc gcgacgacat ccccgtgctc gcggcgagct tcctgcagac ggtgggcgcc
     3301 gtctaccagc gcgacggcct tgcgatgagc ccggcagccg tgcgttggct ccaggggctg
     3361 ccgtggccgg ggaacgtccg gcagctgcgc cagtggatcg agcgaacagt gctcgtgtcc
     3421 gccaggccgg tgctcgaggt cgaggacttc caggcgactt ccgagatgga gccccccgcg
     3481 agccgcgacg acgggctgcc gacggtcggc agcatgaccc tcgacgagat cgagaagggg
     3541 atgatccaga agtcgctgcg gcaccacggt ggcaacatca gccgcgtcgc cgagtcactc
     3601 gggttgagcc gcgccgccct ctaccgccgc ctcgagaagt acgggatcga gacgtgacgc
     3661 tcagccgctg gctcatcgcg tacctggtcg tcctccacct gctggtcgcc gcccttgcct
     3721 tcgtcttcct ccgcgcgagc ccctggtggc tcgtccccat cgagctgctg ctggtcatct
     3781 cgctcgccct cggcctctac ctggtgaacc ggatcttcgg cgcgctggcc ctggtgcgcg
     3841 aggcgcagca gctgctcgac gacggcgact tcatgtcgcg ggtctgcgag gtgggacagc
     3901 ccgaggtgga ccggatcatc cggctgttca accggctcaa cgaccggctg cgcgaggagc
     3961 gcgtgcagct gcaggaacag caccacttcc tgggccgaat gctcgaggta tcgccgctcg
     4021 gggcgctctt cctcgacttc gacgggcggg tcgctcacgc aaacccggcg gcggagcggc
     4081 tcctcggcca gggcttcgcc gacctggcgg gccgcgccct cgcggaggtg ggagggccgc
     4141 tcgccgaggc ggccgccggg ctgccggccg gccagtccac ggtcgtctcg ctctggggcg
     4201 gccgccgcgt tcgctgccac cacgggtcgt tcctcgaccg cggcttcgcg agaagctacg
     4261 tgctgttcga ggagctgacc gaggagctgc ggcagttcga aaaggcggca tacgggaagc
     4321 tgatacggac catgtcgcac gaggtgaaca actcggtggg ggcctcgaac tcgctgcttc
     4381 actcgtgcct caactacgcc ccggaactgc cgcccgagca ccgcgccgat ttcgagtcgg
     4441 ccctctcgat cgccatcgac cgcaccgatc agctgaacgc gttcatgcgg agcttcgccg
     4501 acgtaatccg gctgccgccc cccgcccggc acccgtgcga cctgcgcgag ctggtggccg
     4561 gcgtcgagcg gctggtgcgc gcgcagtgcg aggagcgccg ggtggcgtgg tgctgggacg
     4621 tcgaggagga gccggggacc gtctcgctcg accgcgcgca gatggagcag gtcctgctga
     4681 acgtggtgaa gaacgccctc gaggccgtcg gcgaagacgg gacgatcgcc gcccgcttca
     4741 cccgccgcga agggcgcccg gcgctcgtca tcgaagattc cggcccgggg ctctcggccg
     4801 acgcccgtca gaacctcttc acacccttct tcagcaccaa ggagtacggc cagggcatcg
     4861 gcctgaccct cgtgcaggag atcctggggc agcaccgctt ccagtactcg ctcgacagcc
     4921 ctcccggcgg ccccacgcaa ttcacgatcg tattctgatc agatcgtgca gaaggtgcag
     4981 atggtgcagg gggtgctgaa ggtgcagtgc tgagggtgcc ggaggtgctg gtgccggagg
     5041 tgctggtgcc ggaggtgctg gtgccggag
//
LOCUS       DAOPQX010000267         5064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18004_length_5064_cov_4.939110, whole genome shotgun sequence.
ACCESSION   DAOPQX010000267 DAOPQX010000000
VERSION     DAOPQX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18004_length_5064_cov_4.939110"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /gene="gpmI"
                     /locus_tag="PKK95_12905"
     CDS             complement(<1..1400)
                     /gene="gpmI"
                     /locus_tag="PKK95_12905"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOC19167.1"
                     /translation="MTDLRLDPIPGYAAFPGPVVLLILDGVGLGRRDESDGVFLAYTP
                     VLDALLAEPLSTRLKAHGTAVGLPSDDDMGNSEVGHNALGAGRVFAQGARLVNEAISG
                     GALFQGASWRAATARGLAGGTLHFIGLLSDGNVHSHISHLFAMLGRAADEGVRRVRVH
                     PLLDGRDVGERSALTYLDALEAELQRLNARGLDYQVASGGGRMVTTMDRYNANWAVVE
                     RGWKAHVLGVGRRFGSAREAVETYYREDPAATDQYLDSFVVARDDTPVGPIEDGDAVV
                     FFNFRGDRAIEISRAFEEPAFAEFDRVRVPDVFYAGLMQYDGDALIPKHFLVEPPAID
                     QTISQYLCAAGVTSFAISETQKYGHVTYFWNGNNSGYVDERLERYEEVPSDRVVFDQR
                     PWMKAAEITDATIAAIRSGRYKFVRVNYANGDMVGHTGNVPAIRVAVEAVDLALQRLL
                     PVVRAAGGVLVVTADHG"
     gene            1490..1840
                     /locus_tag="PKK95_12910"
     CDS             1490..1840
                     /locus_tag="PKK95_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19168.1"
                     /translation="MEQSTASPDTTTWQRLDAAVRPGGALPAEPPRSTATGRIGAAVG
                     TLGGRLRSPSVTGRVGAADAVGRGLQRAGAYFDTHDLPDIRNDLERLIVRRPVASVLL
                     ALGVGYLAGRAVWR"
     gene            1845..2243
                     /locus_tag="PKK95_12915"
     CDS             1845..2243
                     /locus_tag="PKK95_12915"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOC19169.1"
                     /translation="MAEERDQASFASLLSGAINDVRELFRQEVSLARAEIRQEVTNAK
                     SAAVKLGAAAVTGLFATLLLLIALARGLSDLFAMPVWAGFTIVGGVLAIAAGVLVAVA
                     WPNIKSVSPMPERTVRTMKENLEWVKRQTS"
     gene            2219..2449
                     /locus_tag="PKK95_12920"
     CDS             2219..2449
                     /locus_tag="PKK95_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19170.1"
                     /translation="MGEAADKLSQPERIEQRIEQTRSRLDDKLSAIEQRARDAVSVRR
                     RVAGRPWTVVGGAAAVGLAAGLVKGWRGRDDR"
     gene            2449..4203
                     /locus_tag="PKK95_12925"
     CDS             2449..4203
                     /locus_tag="PKK95_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC19171.1"
                     /translation="MGSAWDRSSLIDWDNAAHEVALLDWRARSREWGLVPLVDVCLPL
                     RLAAASATTDPPSFLTPGAEGATRDTASVCRRLLEEDDPEAMKPHHQIGLQREAGEPR
                     GEGGQGGDDEEDEDLEDGDEEDLEEGEEEEASEAEGAAEAEPQEAAAEAGPGEETDLV
                     RAYFRQISRWKLLRPEQEAELGRRIEEARAGLQGALARIPPALDTLLALADRVRRGEA
                     SVAELVLFPDGGELVEERVRPVMRALAQAARLERCRQHYASRPADASRLERVEALLAR
                     LVAGLPLRPVFVDELMVRVNELAHRHGMPVDAPHSEPAGGAARADAPPQHLDLGLPVP
                     LFRERLLEAGRHHERIVAAKRELLEANLRLVVSIAKRYQNRGLSLLDLIQEGNLGLMK
                     AVDRFQYRRGFRFATYATWWVRQAVSRAVADYGRTIRLPVHVIEMLNRLARAQRELRE
                     QTGNEPTPEELGARLDMPVGKVQLLLEAVRLPYSLDMPVGEDEETGLGALLEDPSRPS
                     PEARVLERDLADRIERALDVVSEREREILRLRYGIGTGEERTLEEIGRRLSITRERVR
                     QLEARALEKIRRHADRAA"
     gene            complement(4257..>5064)
                     /locus_tag="PKK95_12930"
     CDS             complement(4257..>5064)
                     /locus_tag="PKK95_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19172.1"
                     /translation="GTTASRPEWLRLSRSGDTITSFVSNDGTTWTPVGSTTVALGSSI
                     YVGLAVTSHTISALNTAQFDQVTVTASSAPPPPPPSSAGDIVIYASDVAASSVHGAWS
                     IGSDARSPNGVALVSTDTGFAAADAPLAGPAHYVDVTFSAPAGTPYRVWLRLKALNNS
                     KFNDAVWVQFSDARVNGSPAYAINSTSGLLVNLATSGTATSLNNWGWQNGAYWLSQAT
                     AVTFASSGSHTMRIQLREDGVSLDQIVLSPSRYFNSAPGGPTNDTTIVSK"
BASE COUNT          746 a         1747 c         1864 g          707 t
ORIGIN      
        1 ccatgatcgg cggtcaccac cagcacgccc cctgccgccc gcacgaccgg aagcagccgc
       61 tggagcgcca ggtcgaccgc ctcgaccgcg acccggatgg ccggcacgtt gcccgtgtgc
      121 ccgaccatgt cgccgttcgc gtagttcacc cggacgaact tgtagcgccc cgaccggatc
      181 gcggcgatgg tcgcgtcggt gatctcggcc gccttcatcc acggccgctg gtcgaagacc
      241 acgcggtcgg aggggacttc ctcgtagcgc tcgagccgtt cgtcgacgta ccccgagttg
      301 ttcccgttcc agaagtaggt gacgtggccg tacttctgcg tctcggagat ggcgaacgag
      361 gtcacgcccg cggcgcagag gtactgggag atcgtctggt cgatcgcggg cggctcgacc
      421 aggaagtgct tcgggatgag ggcatcgccg tcgtactgca tcagtccggc gtagaagacg
      481 tcgggcaccc gcacccggtc gaactcggcg aaggccggct cctcgaaggc gcgcgagatc
      541 tcgatcgcgc ggtcgcccct gaaattgaag aagaccaccg cgtcgccgtc ctcgatgggg
      601 ccgaccggcg tgtcgtcccg ggccacgacg aaactgtcca ggtactggtc ggtcgcggcc
      661 gggtcttccc ggtagtaggt ctccaccgcc tcgcgcgcgc tgccgaagcg gcggcccacg
      721 ccgagcacgt gggccttcca cccccgctcg accaccgccc agttggcgtt gtaccggtcc
      781 atcgtcgtca ccatgcggcc gccgcccgac gccacctggt agtcgaggcc gcgcgcgttc
      841 aggcgctgca gctcggcctc cagggcgtcg aggtaggtca gcgcgctccg ctcgcccacg
      901 tcgcggccgt cgagcagcgg gtgcacgcga acgcgccgca cgccctcgtc ggcggcccgg
      961 ccgagcatcg cgaagaggtg ggagatgtgg ctgtggacgt tgccgtccga gagcaggccg
     1021 atgaagtgca gggtgccgcc ggccaggccc cgcgcggtcg ccgcgcgcca cgacgccccc
     1081 tggaagagcg ccccgcccga aatcgcctcg ttcaccaggc gggccccctg cgcgaacacc
     1141 cgcccggcgc cgagcgcgtt gtgccccacc tcggagttac ccatgtcgtc gtcggacggc
     1201 agccccaccg cggtgccgtg cgccttgagg cgcgtcgaca gcggctcggc cagcagggcg
     1261 tcgagcaccg gcgtgtaggc gaggaagacg ccgtcggact cgtcgcggcg gccgaggccg
     1321 acgccatcga ggatgagaag gaccaccggg ccgggaaacg ccgcgtaccc gggaatcgga
     1381 tcaagtcgaa ggtcggtcat gcgttctaca gtaaccgatc cggtcgggga cgtgatccgg
     1441 cagttggcat ccaggctgca agccgattct gtcgaggaga gcgcatctca tggaacagtc
     1501 gactgcttcg ccggacacga ccacgtggca gcggctcgac gccgccgttc ggcccggcgg
     1561 ggccctgccc gccgaaccgc cccgttccac ggccaccggc cggattgggg ccgccgtggg
     1621 aaccctggga ggacggctcc gatcgccgtc ggtcaccggg cgggtcggcg cggcggacgc
     1681 ggtcggccgc gggctgcagc gcgcgggggc gtacttcgac acgcacgatt tgcccgacat
     1741 ccggaacgat ctcgagcggc tcatcgtgag gcggccggtc gcctcggtct tgctggcgct
     1801 gggcgtcgga tacctggccg ggcgcgccgt gtggaggtag gggaatggcc gaggaacggg
     1861 accaggcgtc gttcgcctcg ttgctgagcg gcgcgatcaa cgacgttcga gagctcttcc
     1921 ggcaggaagt ctcgctcgcg cgggccgaga tccggcagga ggtgaccaac gccaagtcgg
     1981 cggccgtcaa gctcggcgcc gcagccgtca ccgggctgtt cgcgacgctg ctcctgctga
     2041 tcgccctggc gcgcggcctc agcgacctgt tcgcgatgcc cgtgtgggcc ggcttcacca
     2101 tcgtcggggg agttctggcg atcgcggcgg gcgtgctggt ggcggttgca tggccgaaca
     2161 tcaagtcggt gagcccgatg cccgagcgca cggtgcgcac gatgaaggag aatctcgaat
     2221 gggtgaagcg gcagacaagc tgagccagcc ggagcggatc gagcagcgca tcgagcagac
     2281 gcggtcgcgc ctcgacgaca agctcagcgc catcgagcag cgggcccgcg acgccgtgtc
     2341 ggttcgccgt cgcgtcgccg gacggccgtg gaccgtcgtc ggcggcgcgg ccgccgtggg
     2401 cctcgccgcc ggcctcgtga agggatggcg cgggagggac gaccgctagt gggttcggcc
     2461 tgggaccgct cgtcgctgat cgattgggac aacgccgcgc acgaggtggc cctgctcgac
     2521 tggcgcgccc gcagccgcga gtggggtctc gtgcccttgg ttgacgtgtg cctgcccttg
     2581 cgcttggcgg cggcctccgc caccaccgac cctccttcct tcctgacgcc tggcgctgaa
     2641 ggggcgaccc gggacactgc gagcgtctgc cgccggctcc tcgaggaaga cgatccggag
     2701 gccatgaaac cccaccacca gatcgggctt cagcgcgagg cgggcgagcc tcgcggcgag
     2761 ggagggcagg gaggcgacga cgaggaggac gaggacctcg aagacggcga cgaggaggac
     2821 ctggaggaag gcgaggagga ggaagcgagc gaagccgagg gggcggcgga ggcggaaccg
     2881 caggaggctg ccgcggaggc cggcccgggc gaggaaaccg acctcgtgcg tgcgtacttc
     2941 cggcagatct cgcgctggaa gctgctgcgg ccggagcagg aagcggagct ggggcgacga
     3001 atcgaggaag cgcgggcagg actgcaagga gcgctcgcgc ggatcccccc tgccctcgat
     3061 acgctgctcg cgctggccga ccgcgtgcgc cgcggcgagg cgtcagtggc ggagctggtg
     3121 ctgttccccg acggcggcga actggtggag gagcgcgtgc ggcccgtgat gcgggccctc
     3181 gcccaggcgg cgcgcctcga acggtgccgc cagcactacg ccagccggcc cgccgacgcg
     3241 agccgcctcg agcgtgtcga ggcgttgctc gcccgcctgg ttgcgggact cccgcttcgc
     3301 cctgtcttcg tggacgaact gatggtgcgc gtgaacgagc tggcacaccg ccacggcatg
     3361 ccggtcgacg ccccgcacag cgagccagcg ggcggggctg cccgtgccga cgccccgccg
     3421 cagcatctcg atctcgggct gccggtgccc ctgttccgcg agcggctcct cgaggcgggg
     3481 cgccaccacg agcgcatcgt ggccgccaag cgcgagctgc tcgaggcgaa tttacggctc
     3541 gtggtctcga tcgcgaagcg ctaccagaac cgcgggctct cgctgctcga cctgatccag
     3601 gagggcaacc ttgggctgat gaaggccgtg gaccgcttcc agtaccgccg cggcttccgc
     3661 ttcgccacct acgcaacctg gtgggtgcgg caggccgttt cgcgcgcggt cgccgactac
     3721 gggcggacga tccggctgcc agtccacgtc atcgagatgc tgaaccggct ggcgagggcg
     3781 cagcgcgagc tgcgcgagca aacgggcaac gagccgacgc ccgaggagct gggggcgcgg
     3841 ctcgacatgc ccgtgggcaa ggtgcagctg ctgctcgagg ccgtccggct cccctactcg
     3901 ctcgacatgc ctgtgggcga ggacgaggag acggggctcg gggcgcttct cgaggacccg
     3961 tcgcggccgt cgcccgaggc gcgcgtcctc gaacgcgatc tggccgaccg gatcgagcgg
     4021 gcgctcgacg tcgtgtcgga gcgcgagcgc gagatcctgc gcctccgcta cggcatcggc
     4081 accggcgagg agcggaccct cgaggagatt ggccgccgcc tctcgatcac gcgcgagcgc
     4141 gtgcggcaac tcgaggcgcg tgcgctcgaa aagatccggc gtcacgcgga ccgggcagcg
     4201 taacctctcg ccgggcgacc acaagggtcc tacccgggcg accacaaggg tcgcccctac
     4261 ttactgacaa tcgtcgtgtc gttggtcggc ccgcccgggg cgctgttgaa gtagcgcgac
     4321 ggactgagca cgatctggtc gaggctcacc ccgtcctccc gcaactggat ccgcatggtg
     4381 tgcgagccgc tcgaggcgaa ggtgacggcg gtggcctgcg agagccagta cgctccgttc
     4441 tgccatcccc agttgttcag gctcgtggcc gtgccgctgg tcgccaggtt caccaggagc
     4501 cccgaggtcg agttgatcgc gtaggcgggc gagccgttca cccgcgcgtc ggagaactgc
     4561 acccagaccg cgtcgttgaa cttcgagttg ttcagcgcct tcaggcgcag ccacacgcgg
     4621 tagggcgtcc cggccggggc gctgaaggtg acatccacgt agtgcgcggg gcccgccagc
     4681 ggcgcgtcgg cagccgcaaa gccggtgtcg gtgctgacca gcgccacgcc gttcggcgat
     4741 cgggcgtcgg agccgatgct ccacgcgccg tgcacggagc tggcggcaac gtcgctcgcg
     4801 tagatcacga tgtcgccggc cgaactgggc ggtggcggcg gcggcgccga actggccgtc
     4861 acggtcacct ggtcgaactg cgcggtgttg agcgccgaga tcgtatggct cgtcaccgcg
     4921 aggcccacgt agatcgacga gccaagggcg acggtggtgc tcccgaccgg cgtccacgtg
     4981 gtcccgtcgt tcgacacgaa cgacgtgatg gtgtcgcccg aacgcgagag ccgcagccac
     5041 tccggccggc tggcggtcgt cccc
//
LOCUS       DAOPQX010000268         5022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18186_length_5022_cov_4.623717, whole genome shotgun sequence.
ACCESSION   DAOPQX010000268 DAOPQX010000000
VERSION     DAOPQX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18186_length_5022_cov_4.623717"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKK95_12935"
     CDS             complement(<1..745)
                     /locus_tag="PKK95_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOC19173.1"
                     /translation="MGRSFRPIIIAAGLLAMAAAAWWLLPRPGGRATGHPATRPNVLL
                     VSIDTLRADRTGGALTPSLNALAARGTRFLNARSPVPLTLPAHASLMTGLLPPRHGVR
                     LNGAHRLAAGTPTLATVLRGAGYRTGAFVGAYVLDPRFGLAEGFDTYDAEIARRDEVA
                     GEPEAERRGEAVVDRAVAWLASQPPGAPFFLWVHLYDPHAPYDPPSGWTERAGGNEYD
                     GEVAYADAQLGRLLESLASQGQASPTVIVV"
     gene            complement(827..3754)
                     /locus_tag="PKK95_12940"
     CDS             complement(827..3754)
                     /locus_tag="PKK95_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC19174.1"
                     /translation="MRRMVLVSLILLCVAAVPALAQQTAGNIAGRVTDEQNAALPGVS
                     VTAREVATGFVRTETTDGQGLYRLMALPVGTYDIRYELASFAVLERKGVVVNVTQTVT
                     LDVGLKLASMSETVTVTGETPLIETTNSAIGGVVEVGRIESMPLNGRQFANLAVTIPG
                     VALGFHSDPTKSTQFSPQINGGNGRNVNYQIDGGDNNDDTVGGLLQLFPLEAIQEFAF
                     VTSRSKAEFGRSQGGVMNIVTKSGTNQYRGSWFTMFRDKSMNATTQTEKASDLPKQDY
                     RRYQYGGSLGGPMILDRIHYFAAFERTQQDTRQAVDTLGLFPELDGVYDTPYRETLVT
                     TKVTMNLSPKHFVSVRYGRNQNSQPYGASPNSPPSSWGDSKNTFNSVNVNHNWVLGGS
                     KLNEFIFQYATFLNGISANSTDPAQAFPNGVYVGQSVNTPQMTNQKKWQFRDDFSWHL
                     TGGGGLGHDFKVGVNFINEPRLYVTFESGKGVMYYSHMDDTIDGPLAAVSMADGEAKA
                     NIPMKQYGMYFQDDWRLTNDLTLNLGVRYDYLSGYQYDQSNNPNFVKIQAAGKAGLLK
                     GIKGLENAGLDPEEDKSNIQPRIGAVWDLRGDGRDVIRAGWGIYMDMAYTNSNGLFAA
                     FDAQGRFGDVIDISDSTGIRNPDGSFYRIGQPLSNILGQNQADPNELPLYGQWVDPRL
                     KMPRTYQTAVGWSHQLTSSTVFTADFVRADGRHLNTRSVINTYIPGTRTRRLTFLNLQ
                     PNSSSTRGAISQAKSRYVAGIFGLKRRMSRGFDLTVTYTLSSAKSHLGNGTDELNTDV
                     LQDHTKLFDDPKTFGPAGRTDARHKGTIASVIQTRGFTIAPMFTFRSRLPVATYHGQD
                     LNLDSVINDLPERAYQFTSIGEAPKDIGACETWNCSRGAWRTQFNLRVSRSFRLFQSA
                     HVEAIGEIFNLFNAKNPSGFLTRQFFGTGAPNPDFMQPTEFAGDFQNPEQRVGQIGFR
                     FSF"
     gene            complement(4059..>5022)
                     /locus_tag="PKK95_12945"
     CDS             complement(4059..>5022)
                     /locus_tag="PKK95_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19175.1"
                     /translation="VARLLGVEPIESDGIDLSPSFGKRALPVRDLYAESYAPLLDFGW
                     SPLRSIRSGRWKAIEAPRPELYDLSADPGEMQPRNEAATLARLLDRVGRFGPAALTEA
                     APADAEARSRLAALGYVQGAPAGEGPRPDPKDRKALAARIQQAVSGEVTGPALKALLE
                     AIVDEDPDNGQARQRLGYVLLEEGRPREAEPHFRAAIAARVPSADPYLGLALCLGRRG
                     DARQALAVLRAGAAVEPGNAVVLANVGIAEAALGRLPESVAALRSALAIDADLHEARF
                     NLARVLARAGQRDEAAREADQLLRRLPAGTPQRREVERLLAAVR"
BASE COUNT          745 a         1695 c         1773 g          809 t
ORIGIN      
        1 ccacgacgat cacggtgggg gacgcctgcc cttgagacgc gagcgactcc agcagccggc
       61 cgagctgagc gtcagcgtac gccacctcgc cgtcgtactc gttgccgccc gcccgctcgg
      121 tccagccaga gggcgggtcg tacggggcgt gggggtcgta caggtgaacc cacaggaaga
      181 acggcgcgcc gggcggctgc gaggcaagcc aggcgaccgc gcggtcgaca accgcctcac
      241 cccgccgctc ggcctccggc tcgcccgcca cctcgtcgcg cctcgcgatc tcggcgtcgt
      301 aggtgtcgaa gccttccgcg aggccgaagc gcgggtcgag cacgtaggcg ccgacgaagg
      361 cgcccgtgcg gtagcccgcc ccgcgaagca ccgtggcaag cgtcggcgtg ccggccgcaa
      421 ggcggtgcgc accgttcaat cgcacgccgt gcctgggcgg cagcaggcca gtcatcagcg
      481 aggcgtgggc gggaagggtg agcggcaccg ggctgcgcgc gttcaggaat cgcgtcccgc
      541 gcgcggcaag ggcgttgagc gacggcgtga gggcgccgcc ggtgcggtcg gcgcgcagcg
      601 tgtcgatgga cacgagcaga acgttcggtc gcgtggcggg atggccggtg gcgcgcccgc
      661 cgggccgcgg cagcagccac cacgcggcag cggccatcgc gaggagcccg gcagcgatga
      721 tgatcggcct gaaagagcgt cccatggcgg gtgagaggcg gcggcgtgcc gcctctcctg
      781 tggagtgtaa gggaaaacgg gcgggcacgg aggcccgccc tgtcgatcag aacgagaagc
      841 ggaacccgat ctggccgacc cgctgctcgg ggttctggaa atcgcccgcg aactcggtcg
      901 gctgcatgaa gtcggggttc ggtgcgccgg tcccgaagaa ctgcctggtg aggaagccgg
      961 acgggttctt cgcgttgaac aggttgaaga tctccccgat ggcctccacg tgcgcgctct
     1021 ggaacaggcg gaagctgcgc gacacccgca ggttgaactg cgtgcgccat gcgccgcggc
     1081 tgcagttcca ggtctcgcag gcgccgatgt ccttcggcgc ctcgccgatc gacgtgaact
     1141 ggtaggcgcg ctccggcagg tcgttgatca cgctgtccag gttgaggtcc tgcccgtggt
     1201 aggtggcaac cggcagcctc gaccggaacg tgaacatcgg cgcgatggtg aaccctctcg
     1261 tctggatgac gctggcgatg gtccccttgt gccgggcgtc ggtccggccc gccggcccga
     1321 acgttttcgg gtcgtcgaac agcttcgtgt ggtcctgcaa cacgtcggtg ttcaactcgt
     1381 cggtgccgtt gccgaggtgg ctcttcgccg acgagagcgt gtaggtcacg gtgaggtcga
     1441 acccccgcga catccggcgc ttcaacccga aaatgccggc cacgtaccgg ctcttcgcct
     1501 ggctgatcgc tcccctggtg gatgaggagt tcggctggag gttgaggaac gtcaggcgcc
     1561 gggtcctcgt cccgggaatg taggtgttga tcaccgaccg ggtgttcagg tgacggccgt
     1621 cggcccggac gaagtccgcc gtgaagacgg tggagctggt cagctggtgc gaccacccga
     1681 cggccgtctg gtaggtccgc ggcatcttca accgcgggtc cacccactgg ccgtacagcg
     1741 ggagttcgtt ggggtcagcc tggttctggc ccaggatgtt ggagagcggc tgcccgatcc
     1801 ggtagaacga gccgtcgggg ttcctgatgc cggtcgagtc ggagatgtcg atgacgtcac
     1861 cgaatcgccc ctgggcgtcg aaggcggcga acaggccgtt cgaattggtg taggccatgt
     1921 ccatgtagat gccccacccg gcccggatga cgtcacggcc gtcgccgcga aggtcccaca
     1981 ccgcgccgat ccgcggctgg atgttgctct tgtcctcttc ggggtcgagc cccgcgttct
     2041 cgaggccctt gatgcccttg aggagccccg ccttcccggc cgcctggatc ttcacgaagt
     2101 tcgggttgtt cgactggtcg tactggtacc cgctgaggta gtcgtaacga acgcccaggt
     2161 tcagcgtcag gtcgttggtg agccgccagt cgtcctggaa gtacatcccg tactgcttca
     2221 tcgggatgtt ggccttggct tccccgtcgg ccatcgagac ggccgccagc gggccatcga
     2281 tcgtgtcgtc catgtgcgag tagtacatga cgcccttgcc cgattcgaac gtcacgtaca
     2341 gccgcggctc gttgatgaag ttcacgccca ccttgaagtc gtggcccaga ccgccgcccc
     2401 ccgtgaggtg ccacgagaag tcgtcgcgga actgccactt cttctggttc gtcatctgcg
     2461 gcgtgttgac gctctgcccg acgtagacgc cgttcgggaa ggcctgggcc gggtcggtgc
     2521 tgttggcgct gatgccgttc aggaacgtgg cgtactggaa gatgaactcg ttcagcttcg
     2581 acccgccgag cacccagttg tggttcacgt tcaccgagtt gaacgtgttc ttgctgtcgc
     2641 cccacgagct ggggggcgag ttcgggctcg caccgtacgg ctgcgagttc tggttccgcc
     2701 cgtagcgcac cgacacgaag tgcttcgggc tgaggttcat ggtgaccttg gtggtcacca
     2761 gggtctcacg gtagggcgtg tcgtacacgc cgtccagctc ggggaacagc cccagcgtgt
     2821 ccaccgcctg cctggtgtcc tgctgcgtgc gctcgaacgc cgcgaagtag tggatgcggt
     2881 cgaggatcat cggcccgccg agcgagccgc cgtactgata cctgcggtag tcctgcttgg
     2941 gcagatcgct tgccttctcg gtctgcgtcg tggcgttcat cgacttgtcg cggaacatcg
     3001 tgaaccagct gccgcggtac tggttcgtcc cgctcttggt gacgatgttc atcacgccgc
     3061 cctggctgcg gccgaactcc gccttcgagc gcgaggtgac gaacgcgaac tcctggatgg
     3121 cctcgagcgg gaacaactgc agcaggccgc cgacggtgtc gtcgttgttg tcgccgccgt
     3181 cgatctggta gttgacgttc cggccgttcc ccccgttgat ctgcggcgag aactgcgtgc
     3241 tcttggtcgg gtcggagtgg aaccccagcg ccacgccggg gatcgtgacg gccaggttcg
     3301 cgaactgccg gccgttgagc ggcatcgact cgatgcgccc cacctcgacg acgcccccga
     3361 tcgccgagtt ggtagtctcg atcagcggcg tctcgccggt caccgtcacc gtctccgaca
     3421 tcgatgccag cttgagcccg acgtccagcg tgacggtctg cgtgacgttg accacgaccc
     3481 cctttcgctc gaggacagcg aaggacgcca gctcgtacct gatgtcgtag gtgccgaccg
     3541 gcagcgccat gaggcggtac agcccctggc cgtcggtggt ctcggtgcga acgaacccgg
     3601 tcgccacctc ccgcgccgtg accgagacgc cggggagggc cgcgttctgt tcgtcggtaa
     3661 cccggccggc gatgttaccg gccgtctgct gcgcgagcgc cgggaccgcg gcgacgcaca
     3721 gcaggatgag cgagacgagc accatccttc gcatgcccga cctcctgagg aaagaaccgc
     3781 tgcgacgaga atgaggcgac cgacacgctg tcgaatcgaa ggaccgccga ccggacgtgg
     3841 gaagggccgc cgccgaacgg ggagacgccg cgcgcgagta cgccgccgaa cgaaccgacg
     3901 ggcgccgccg ggagtgggtg ggtggggacg gtcaagagac taccgcgccg cggcggccgc
     3961 gtccagtgaa atgcagaccg aatccggtgt gacgaatcgc gccccgcact ggactccaac
     4021 catccaactt caaagcggtc tgagccggtc ccgctcgatc agcgaactgc ggccagcagc
     4081 cgctcgacct cgcgccgctg cggagtcccg gccggcaggc ggcgcagcag ttggtccgct
     4141 tcccgggcgg cctcgtctcg ttgtcccgcg cgggcgagca cgcgcgccag gttgaagcgc
     4201 gcctcgtgaa ggtcggcgtc gatcgcgagg gccgaccgca gggcggccac ggactcgggc
     4261 agtcgtccga gcgcggcctc ggcaatcccc acgttcgcca gcaccaccgc gttgccgggc
     4321 tccaccgccg cccctgcccg cagcacggca agggcctggc gggcgtcccc gcgccggccc
     4381 aggcagaggg ccaggcccag atagggatcg gccgatggaa cgcgcgccgc gatcgccgca
     4441 cggaagtggg gttcggcctc gcgcgggcgc ccctcctcga ggagcacgta gccgaggcgc
     4501 tggcgcgcct gcccgttgtc ggggtcctcg tccacgatcg cctcgagcag ggccttcagc
     4561 gccggcccgg tcacctcacc cgacaccgcc tgctgaatcc gcgcggcgag cgccttgcgg
     4621 tctttcggat cgggacgagg tccctcgccg gcgggagcgc cctgcacgta ccccagcgcc
     4681 gccaggcgcg atcgcgcctc cgcgtcggcg ggcgccgcct cggtgagagc ggcaggcccg
     4741 aagcgcccga cccgatccag caggcgcgcg agcgtcgctg cctcgttgcg cggctgcatc
     4801 tctcccgggt cggccgacag gtcgtacagc tcgggccggg gcgcctcgat cgccttccag
     4861 cgcccggagc ggatgctgcg gagcgggctc cagccgaagt cgagcagcgg cgcgtacgac
     4921 tcggcgtaga ggtcgcggac gggcagcgcg cgcttcccaa aggaaggcga gaggtcgatc
     4981 ccgtccgact cgatcggctc gacgcccagc agccgagcga cg
//
LOCUS       DAOPQX010000269         5006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18264_length_5006_cov_4.284589, whole genome shotgun sequence.
ACCESSION   DAOPQX010000269 DAOPQX010000000
VERSION     DAOPQX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18264_length_5006_cov_4.284589"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKK95_12950"
     CDS             complement(<1..188)
                     /locus_tag="PKK95_12950"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19176.1"
                     /translation="MPNPVNVLCIGAGYVGGPTMAVIADRCAHARVTVVDINAERIAA
                     WNSDTLPIYEPGLDEVVRR"
     gene            complement(302..859)
                     /locus_tag="PKK95_12955"
     CDS             complement(302..859)
                     /locus_tag="PKK95_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(635..637),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOC19177.1"
                     /translation="MRIAPARRASALLVLAALFCGVRTVAAQERPLQPGDPAPAFTLA
                     ASDGRTYSLADFLGVRPVVVAWFPKLFATLUTRDCEPRRERGSEVRPYDVAFFEASVY
                     SVETNRQLADMLDLPHPILSDPERKVARAYGVVTDERPIPRRWTFYIGTDGKILYIDK
                     QVTADGHGEEVPERLEELGIARRRE"
     gene            complement(956..1627)
                     /locus_tag="PKK95_12960"
     CDS             complement(956..1627)
                     /locus_tag="PKK95_12960"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOC19178.1"
                     /translation="MAVGTAAQALTDEEVVDRVLHGETALFEILVRRHNTRVYRAVRA
                     VLGDEREVEDAMQQAYLDAYAHLAQFAGRARFSTWLTRIAVNEAIGRARRQGVRMETG
                     FPDEDGREDADRRFAAADPSPEHRAFSFELRALLETAIDTLAPGYREVFMLREVEGMS
                     TAEVAEALGLTDEAVKTRLHRARGLLKDDLFSRTGTAAADAFQFHAPRCDRVVEGVMG
                     ELAGR"
     gene            1764..2213
                     /locus_tag="PKK95_12965"
     CDS             1764..2213
                     /locus_tag="PKK95_12965"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOC19179.1"
                     /translation="MRHELETAYWALRPAIGATAFLAGLDKYFNLLANWTDYVSPLAL
                     EVLPFGATTLMQVVGVVEIAVGLAILLGWTRLGAYVASAWLAAIALNLVTTGRYFDIA
                     VRDLVMAVAAYTLARLAEVRGTAEAAVPQHTRIFEGNSEHARDRLRA"
     gene            2496..3320
                     /locus_tag="PKK95_12970"
     CDS             2496..3320
                     /locus_tag="PKK95_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19180.1"
                     /translation="MPPLHRRTGIAVVVLAFAASLLVPAAGIAQQAQQTPQAQQAPQP
                     GAMSQPGASATELYHVHFVKATPGKLTELIAAYQDSPVPPGEPGRPLVFRHLQGDDWD
                     LLVLSPLGKEETLSTSASAEQQQWSERTRGLRAQHADTFTAGPAWADLQARLNDAPRA
                     TGTAGTAAEPTAGRLYIVTTYRSLPGHRDQLASVLNRVAALYPDRRTILQHVEGAPWE
                     FVMLSRYDSWNAIGTDETAPPENLRKQGFASQEAIGTELRQHVAEHRDTIVRLATK"
     gene            3451..3526
                     /locus_tag="PKK95_12975"
     tRNA            3451..3526
                     /locus_tag="PKK95_12975"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3484..3486,aa:Lys,seq:ctt)
BASE COUNT          732 a         1736 c         1719 g          819 t
ORIGIN      
        1 cggcggacga cctcgtcgag gccgggctcg tagatcggca gcgtgtcgct gttccaggcg
       61 gcgatacgct cggcgttgat gtcaacgacc gtgacccggg cgtgcgcgca ccggtcggcg
      121 atgacggcca tcgtcgggcc gccgacgtag ccggccccaa tgcagaggac gttcacgggg
      181 ttgggcataa cctagcgaga ataggcggag ctgcgcggcc ccgtcaacgg gatcagggtc
      241 ccaatcagca gcgccggctt ccgtgccggc gagtaggacc ggcgtcccgc ccgtgtcggc
      301 cctactccct ccgcctcgcg atccccagtt cctcgagccg ttcgggcacc tcttcgccgt
      361 ggccgtcggc cgtgacctgc ttgtcgatgt agaggatctt tccgtcggtg ccgatgtaga
      421 aggtccagcg gcgtgggatg gggcgctcgt ccgtcacgac gccgtaggcg cgcgccacct
      481 tcctctccgg gtcgctcagg atcgggtgcg gaaggtcgag catgtcggcg agctgccggt
      541 tcgtctccac cgagtagacg ctcgcctcga agaaggccac atcgtagggg cgcacctcgc
      601 tgccgcgctc acggcgcggc tcgcagtccc tggttcaaag ggtggcgaac agcttgggga
      661 accacgcgac gaccacgggg cggacgccga ggaagtcggc aaggctgtag gtgcggccat
      721 cgctggcggc aagcgtgaag gcgggcgccg ggtcgcccgg ctggagcggc cgttcctgag
      781 ccgcgacggt tcgcacgccg cagaacagcg ccgcaagcac gagaagcgcg gaagccctcc
      841 gcgccggtgc aatcctcatt gggtcagtat agcgaacgct ccagcccctg gcctcctggc
      901 ccctggcagg ttgcctacat ccttcctttt gcatactgca ttcaggccgc gctcttcagc
      961 ggcctgccag ctcccccatc actccctcca ccacccgatc gcagcggggg gcgtggaact
     1021 ggaaggcatc ggcggcggcg gtgccggtcc tcgagaagag gtcgtccttc agcaggccgc
     1081 gggcgcggtg caggcgtgtc ttgacggcct cgtcggtgag ccccagcgcc tcggcgacct
     1141 ccgcggtgga catcccctcg acctcgcgca gcatgaacac ctcgcggtag cccggggcca
     1201 gcgtgtcgat cgcggtctcg agcagcgcgc gcagctcgaa cgagaaggcg cggtgctcgg
     1261 gcgacgggtc tgcggcggcg aagcggcggt cggcatcctc gcgcccgtcc tcgtccggaa
     1321 agccggtttc catgcggacc ccctgccgcc tggcgcggcc gatcgcctcg ttgacggcga
     1381 tcctcgtcag ccacgtcgag aaccgcgcgc gcccggcgaa ctgcgccagg tgggcgtagg
     1441 cgtcgaggta cgcctgctgc atcgcgtcct cgacctcgcg ctcgtcgccg agcacggcgc
     1501 gcacggcccg gtagaccctc gtgttgtggc ggcgcaccag gatctcgaag agcgccgtct
     1561 cgccgtggag gacgcggtcc accacctcct cgtcggtcaa cgcctgagcg gccgtgccca
     1621 cagccatgct gccctccttc cccattagag atcgccggtg gcgaaaggtg acacggcagt
     1681 tgcggggccg ccatcgggcg gcaagtgaag ctctgccacg cgtgcgccgc cgcgcgctca
     1741 gtgctggcag gaggtgaaga gcgatgagac acgaactgga gaccgcgtac tgggccctgc
     1801 ggccggcgat cggggccacc gccttcctgg ccggattgga caagtacttc aacctgctgg
     1861 cgaactggac cgactacgtc agcccgctcg cgctggaggt cctgccgttc ggcgcgacga
     1921 ccctgatgca ggtcgtcggc gtggtggaga ttgcggtcgg gctcgcgatc ctgctgggct
     1981 ggacccgcct cggggcgtac gtggcgagcg cctggctggc cgcgatcgcg ctgaacctgg
     2041 tgaccaccgg gcggtacttc gacatcgcag tgcgcgacct ggtgatggcg gtcgccgcgt
     2101 acaccctggc ccggctggcc gaggtgcgcg gcacggcgga ggcggcggtt ccccagcaca
     2161 cgcggatctt cgaggggaat tcggagcatg cccgcgacag gctgcgcgcg tgagcgtcgc
     2221 ccgcactgtc gtgtgccgct acacccgggc ggctgtcggc ggcacgcggc gggcgaacgc
     2281 cggcggcggc gcgatcgcgc gtgcagaatc acgcgtcttc ccgtcgcgcg cccgcggacc
     2341 cgggtttgca tctcggcggc cggcgcgctc cagtggacgg gcgttcccgc ccgtcggtcg
     2401 tagcaggggc gtcctctcac agttggtagg acgggcgtcc tttgcccgtc cccgtgatct
     2461 cactgtcgag ccggagacgg ctcgaaggag tctccatgcc cccccttcat cgacggaccg
     2521 gcattgccgt ggtcgtcctc gcgttcgccg cctcgctcct cgtccccgcc gctggcatcg
     2581 ctcagcaggc tcagcaaacg ccgcaggccc agcaggctcc gcagccgggc gcgatgtcgc
     2641 agcccggggc ctcggcgacg gagctctacc acgttcactt cgtgaaggcg acgcccggca
     2701 agctcacgga actgatcgcg gcgtaccagg actcgccggt gccgccgggc gagcctggcc
     2761 gccccctcgt tttccggcac ctgcagggcg acgactggga tctcctcgtg ttgtcgccgc
     2821 tgggcaagga ggagacgctc tcgacgtccg cgagcgccga gcagcagcag tggagcgaac
     2881 gcacgcgcgg gctgcgggcg cagcacgcgg acaccttcac cgcgggtccg gcgtgggcgg
     2941 atctgcaggc acggctcaac gacgccccgc gcgcgacggg gacggcggga acggcggcag
     3001 agcccacggc cggacgcctc tacatcgtca cgacgtaccg ttcgctcccc gggcaccgcg
     3061 atcagctggc gagcgtgctg aaccgggtcg cggcgctcta ccccgatcgc cgcaccatcc
     3121 tgcagcacgt cgagggggcg ccgtgggagt tcgtgatgct gtcgcgatac gactcgtgga
     3181 acgcgatcgg caccgacgaa accgcgccgc ccgagaacct gcgcaagcag gggttcgcca
     3241 gtcaggaagc catcgggacg gagctgcggc agcacgtggc cgagcaccgc gacacgatcg
     3301 tgcggctggc gacgaaatga agtatccgtg gaacacaccg cggcgcgagc aattgatccg
     3361 cggccggggc ggttgctata ctaggtagcc gccgccgcgt gcggcggcta cgcccgtccg
     3421 ctcctggcga acgggccctc caccctgggt gggccgctag ctcaattggc agagcagcag
     3481 actcttaatc tgcgggttgt aggttcgatt cctacgcggc tcaccaaaat ttcccgagcg
     3541 ctttcgccag gcgctaagta cctgaaaagc agtgccgttg cggcggttcg attgaaccgt
     3601 tttccgtcgt acgcgatccc ttccgggaag aacagctgct gcaggcgctg cttgtagtcg
     3661 agcgacgcct gaacccacag gtccgcggcg cgcggcagga tgcgttctgc gaaggccagg
     3721 atgccctgga cgtcgagttc gtcgacggcg tcggcatgct ggtccatctg cgcgagcgtc
     3781 agctcctcgc gcagcctgtc gcgttgccgc tcgtaggtct tgccatcgat cgtctgcgca
     3841 aacaggaacg cgtcatccag gcggtcgagc ttctgttgga tggccttgac gcgcttctcc
     3901 tggtctgccg tccggcccct cgcttcggcc cgctgctgtt cccacacgtg caggatgcga
     3961 tccttcacga gccgcatgta gccaggggac ggctgcaaga gcgcgagttc gtcagcgaac
     4021 gcgccttcga gagtggcctt gccgacgttc accgctcgac actgtcgctg acagtggtag
     4081 tacgcgtagt gcccgcttct ccccttcgac cagcttcccg tcagcggtcg cccgcacaca
     4141 tcgcagcgga cgaagcctcg cagtgggaaa tcagggtggt tccgctggcg cggcccggac
     4201 acgaccacgc gtccatcgag cacggcctgt gcgcggtaga acgtcgcatc gtcgacgagc
     4261 ggctcgaagt cgccgcgccc ggacacgccg tagtcggggc tctcgaccct gccgatgtag
     4321 agtgggttcc gcatcatctg gccgaggctc tgcggagaga ttctcagccc cttcctgctg
     4381 cgaagtcccg cctcggtggc gcgcgcgatc acttcctgct tcgtgtaacg gcccgtcgcc
     4441 aggtcttcga acgctcgtcg caccagcggc ccgcgctccg ggtcgtgcac caggctcgcg
     4501 cccgaccact tcggtgcgtt cagatacccg agcggcgccg ggaacgtcca ccggccaagt
     4561 tccagcgccg cgcgcatgcc ggcacgcgtg cggtccgagc gcacgtcgtt gtcgaactgc
     4621 gcgaaggcgg ccagcacgcc ttccatcagc ttgcccgtgg aagtgtcgtc gatcggctcg
     4681 gtgaccgacc gcagcgagat gccgagtgag ttgaggtggg cgcggagcgc gaagtggtcg
     4741 aatttctccc gtgcgaagcg cgtgagattg tagacgacca cgaagtggac tttgcccttg
     4801 tttgtccggc agtacttcag tagcgcctgg agctggctgc gatccgtcgt cttggcgctc
     4861 tcgccttcct cgtgaaagcg cccgacgatc tcgtagccct gccggcggca gtactcctcg
     4921 caggcgcgga gttgcgtcgg gagactcagg ttctccgtct gctccttcgt gctgacgcgc
     4981 acgtagatca ccgcgccgac catgtc
//
LOCUS       DAOPQX010000270         5005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18267_length_5005_cov_4.122424, whole genome shotgun sequence.
ACCESSION   DAOPQX010000270 DAOPQX010000000
VERSION     DAOPQX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18267_length_5005_cov_4.122424"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1121
                     /locus_tag="PKK95_12980"
     CDS             57..1121
                     /locus_tag="PKK95_12980"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOC19181.1"
                     /translation="MRRRILALAGMSATAAIAIVAAATSVGDPPVQVRMTRDEARQQV
                     DVLVDGKPFTSFIHRPSLRKPVLFPLRTAGGNAVTRGFPLEPRPGERVDHPHQVGLWF
                     NYGDVGGFDFWNNSDAIGAADRPKMGTIVHEEMGALREGEGEGELEARMKWVGSDGKA
                     LLAEHARFVFRGGPGWRSVDRIARLRPLAGRVAMPDNKEGMLGLRVARALEMPSNQPE
                     TFTDAAGRPTSLKVLDNAGVTGRYFTSEGKTGEAVWGTRARWCALSGRLGDEVVTVAI
                     LDHPLNPGFPTYWHARGYGLFAANPLGQNALSGGRETLGFAIEANDTATFRYRILILS
                     EAATKERLEAEYKEFASAYR"
     gene            complement(1215..2573)
                     /locus_tag="PKK95_12985"
     CDS             complement(1215..2573)
                     /locus_tag="PKK95_12985"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOC19182.1"
                     /translation="MRTLGSMAALVLLAAPAQAQDGWRQLFDGRTLDGWQVCNGSAPF
                     VVEGGAIVGRSVVSSPNSFLCTKEAFGDFILEYEARIESEMNSGVMVRGIVDPAVRGG
                     RMHGYQVEIDPSDRAWTGGIYDEARKGWLHTLAGQEAARKAIRKGDWNRFRVEALGSS
                     IRTWVNGVPAANVLDDETARGVIALQVHSIGSDASKAGQAIRFRKIRILTSSPEAQKT
                     PDRGEVPQYNYVPNTLSEREARDGWKLLWDGKTTAGWRGAKLDTFPASGWEIKDGVLS
                     VMATGGAESAAGGDIVTVDRYGDFELIVEFLIAKGANSGIKYFVDPGLNKGEGSAIGC
                     EFQILDDVNHPDAKAGMSGNRKLAGLYDLIPPENVRFNGVGEWNRARIVARGQHVEHW
                     LNGFKTVEYERGTQMWRALVAHSKYTVWPRFGEAPKGHILLQDHGDRVSFRSVKIREI
                     RE"
     gene            complement(2592..3671)
                     /locus_tag="PKK95_12990"
     CDS             complement(2592..3671)
                     /locus_tag="PKK95_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOC19183.1"
                     /translation="MDNKAISSDGALDFLSLGALVHRLDPGVVPFRKANACSIHVSGG
                     EFNCAANLADCFRLKTGIASAMVDYPIGDLIAERVRAMGVRAFYKRFPHDGVRGPNMA
                     TVYSDRGFGVRAPVVFYNRSDEAAARLAPGDFDWASIFGAGVRWFHSGGIFASLSETT
                     AELIIEGMKAARAAGATTSFDLNYREKLWRVSGGAGRATSVINRIVEHVDVLVGNEED
                     LQKGLGIPGPEVAARSKLDPGAFFGMIDRVLDRHPHVKVVATTLREVHSTRRHSWSAV
                     AWIGGNNLQAPTAELDVYDRVGGGDGFASGLFYGLLAGEPPEEALRLGWAHGALLTTF
                     PGDTTMATLDQVRAFARGGSARIQR"
     gene            complement(3705..>5005)
                     /gene="gndA"
                     /locus_tag="PKK95_12995"
     CDS             complement(3705..>5005)
                     /gene="gndA"
                     /locus_tag="PKK95_12995"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HOC19184.1"
                     /translation="QTKAFLDGPGKGKRVAGAESPAALMAMLQLPRRVLMMVPAGRPV
                     DDAIAHLRPHLAPGDILIDGGNSWFLDTERRNRDLAGSDFRYIGTGVSGGEQGALWGP
                     SIMPGGQVEAWEALAPVLRAIAAKAEDGEPCVDYMGPLGAGHYVKMVHNGIEYGDMQL
                     IAEVYDLLQRGLGLPASELSAIFEEWNRGVLRSYLIEITAQVLARVDGETGRPLVDVI
                     LDEAAQKGTGKWTSQNALDLGAPIPTINAAVEGRLISALKVERVAASAVLRGPSPRYQ
                     GPAEALVDAARDALYAAKITSYAQGFGLLRLASKEYNYGLRPATIARIWRAGCIIRAN
                     LLGDIMAAYERDPGLVNLLLDERFREAVESRQAAWRTVVKAAVEGGIPSLALSASLAY
                     FDAYRTERLPANLTQGQRDFFGAHTYRRVDRPGVFHTDWE"
BASE COUNT          732 a         1806 c         1704 g          763 t
ORIGIN      
        1 caggattcag gagtcgggat tcaggattcg ggagccggaa gtcaggtggg gagaaaatgc
       61 gacgcaggat cctcgcgttg gctggcatgt cggcgacggc cgcaatcgcg atcgtggccg
      121 ctgcgacctc cgtcggcgat ccgccggtgc aggtgcgcat gactcgcgac gaggcgcggc
      181 agcaggtgga tgtcctggtg gacggcaagc cgttcacgtc gttcatccac cggccgtcat
      241 tgaggaagcc cgtgctcttt ccgctgcgga cggcgggcgg gaacgcggtc acgcgcggct
      301 tcccgctcga gccgcgcccc ggcgagcgcg tcgatcatcc gcaccaggtg ggcctctggt
      361 tcaactacgg cgacgtgggc ggcttcgact tctggaacaa ctctgacgcc atcggcgccg
      421 ccgatcggcc gaagatggga acgatcgtcc acgaggagat gggcgcgctc cgcgagggcg
      481 agggggaggg cgagctggag gcccgcatga aatgggtggg cagcgacggc aaggcgctcc
      541 tcgccgagca cgcgcggttc gtcttccggg gtgggccggg ctggcgatcc gtggatcgca
      601 tcgcgcggct gcggccgctg gccggccgcg tggcgatgcc cgacaacaag gagggcatgc
      661 tcggcctccg cgtcgcgcgg gcgctcgaga tgccgtcgaa ccagcccgag accttcaccg
      721 acgcggcggg ccgccccacc tcgctgaagg tgctcgacaa cgcgggcgtg accggccgct
      781 acttcacgag cgaggggaag acgggcgagg cggtatgggg cacacgcgcg cggtggtgcg
      841 cgctcagcgg ccgcctgggc gacgaggtgg tcaccgtggc catcctcgac cacccgctca
      901 accccggctt cccgacctac tggcacgccc gcggctacgg cttgttcgcg gcgaacccac
      961 tcggccagaa tgcgctcagc ggcggcaggg aaacactggg gttcgcgatc gaggcgaacg
     1021 acaccgccac gttccggtac cgcatcctca tcctgtccga ggccgccacc aaggagcggc
     1081 tcgaagcgga gtacaaggag tttgcctcgg cgtaccggtg atcccgtcgc agatcctcga
     1141 tcttcgaccc ctgagcttcg tcctgaatcc cgactcctga ctcctgaatc ccgactcctg
     1201 actcccgaat cccgctactc cctgatctcc ctgatcttca ccgaccggaa cgacacgcgg
     1261 tcgccgtggt cctgcagcag gatgtgcccc ttgggggcct cgccgaacct gggccacacg
     1321 gtgtacttgc tgtgcgccac cagcgcccgc cacatctgcg tgccgcgctc gtactcgacg
     1381 gtcttgaaac cgttcagcca atgctccacg tgctgcccgc gggccacgat ccgcgcccgg
     1441 ttccactcgc cgacgccgtt gaagcgcacg ttctcgggcg ggatcaggtc gtagagcccg
     1501 gccagcttgc ggttgccgct catacccgcc ttcgcgtcgg ggtggttcac gtcgtcgagg
     1561 atttggaact cgcagccgat cgccgaccct tcgcccttgt tcaggcccgg gtccacgaag
     1621 tacttgatgc cgctgttggc ccccttggcg atcaggaact cgacgatcag ctcgaagtcg
     1681 ccgtaccggt ccaccgtgac gatgtcgccg ccggcggccg actcggcgcc gccggtagcc
     1741 atcaccgata gcactccatc cttgatttcc cagccgctgg cggggaaggt gtcgagcttc
     1801 gccccgcgcc agcccgccgt cgtcttgccg tcccagagca gcttccagcc gtcgcgggcc
     1861 tcgcgctcgc tgagggtatt ggggacgtag ttgtactgcg gcacctcacc tcggtcgggg
     1921 gtcttctgcg cctcggggct cgaggtgagg atgcggatct tgcggaagcg gatggcctgc
     1981 ccggccttcg aggcgtccga gccgatcgag tgcacctgga gggcaatcac ccccctcgcg
     2041 gtctcgtcgt ccagcacgtt ggcggcgggc acgccgttca cccaggtccg gatcgacgag
     2101 cccagcgcct cgacacggaa gcggttccag tcgcccttgc ggatggcctt ccgcgccgcc
     2161 tcctggcccg cgagcgtgtg gagccagcct ttccgcgcct cgtcgtagat gccgccggtc
     2221 caggcgcggt cggacggatc gatctccacc tggtagccgt gcatccgccc gccgcgcacg
     2281 gccgggtcga cgatcccgcg caccatcacg ccgctgttca tctccgactc gatccgcgcc
     2341 tcgtactcga ggatgaagtc gccgaacgcc tccttggtgc agaggaagct gttgggcgag
     2401 ctcaccaccg accgcccgac gatcgccccg ccctcgacaa cgaagggggc cgacccgttg
     2461 cagacttgcc agccgtcgag cgtgcggccg tcgaagagct ggcgccagcc gtcctgcgcc
     2521 tgagcggggg ccgcgaggag cacgagcgcg gccatcgagc cgagggttct catcaatgac
     2581 atctccacgt cctacctctg gatcctcgcc gagccgccgc gcgcgaacgc gcgcacctgg
     2641 tcgagcgtcg ccatggtggt gtcgccgggg aaggtcgtca gcagcgcccc gtgcgcccag
     2701 ccgagccgca gcgcctcctc cggcggctcg cccgccagca gcccgtagaa caggccggag
     2761 gcgaagccgt ctccgccgcc cacccggtcg tagacgtcga gctcggcggt cggcgcctgg
     2821 aggttgttcc cgccgatcca ggcaaccgcg ctccacgagt ggcggcgcgt cgagtgcacc
     2881 tcgcgcaagg tggtcgcgac caccttgaca tgggggtggc gatcgaggac gcggtcgatc
     2941 atgccgaaga aggcgcccgg atcgagcttc gacctcgcgg ccacctcggg ccccggaatg
     3001 ccgaggccct tctggaggtc ctcctcgttg cccaccagca cgtccacgtg ctcgacgatc
     3061 cggttgatga cggaggtcgc ccggccggct ccgcccgaca cgcgccacag cttctcgcgg
     3121 tagttcaggt cgaacgaggt ggtggccccc gccgcgcggg cggccttcat gccctcgatg
     3181 atcagctcgg cggtggtttc cgagagcgac gcgaagatgc cgccgctgtg gaaccagcgc
     3241 acgccggcgc cgaagatcga cgcccagtcg aagtcgcccg gggccagccg cgccgcggcc
     3301 tcgtccgagc gattgtagaa gacgacgggg gcgcggacgc cgaagccgcg gtcgctgtag
     3361 accgtggcca tgttcgggcc ccggacgccg tcgtgaggga agcgcttgta gaaggcgcgg
     3421 acgcccatcg cgcgcacccg ctcggcgatc aggtcgccga tcgggtagtc gaccatcgcc
     3481 gaggcaatcc cggtcttgag gcggaagcag tcggccagat tggcggcgca gttgaactcg
     3541 cccccgctca cgtggatgct gcaggcgttc gccttgcgga agggaaccac gcccgggtcg
     3601 agccggtgca cgagcgcgcc gagcgacagg aaatcgagcg cgccatcgga cgaaatcgct
     3661 ttgttgtcca ctccacactc ccgtggccgg cacaaaggcc ggccctactc ccagtccgtg
     3721 tgaaacaccc cgggacggtc gacgcggcgg tacgtgtgtg ccccgaagaa gtcgcgctga
     3781 ccctgagtga ggttcgccgg gagccgctcc gtccggtacg cgtcgaagta cgccagcgac
     3841 gcgctcaacg ccagggacgg gatgccaccc tcgaccgccg ccttcaccac cgtgcgccag
     3901 gcggcctgcc gcgactcgac cgcctcgcgg aagcgctcgt cgagcagcag gttcaccagg
     3961 ccggggtcgc gttcgtacgc cgccatgatg tcgccgagca ggttcgcccg gatgatgcag
     4021 ccggcccgcc agatgcgggc gatggtggcc ggcctcaacc cgtagttgta ctccttcgag
     4081 gccagccgca gcagcccgaa cccctgcgcg tacgaggtga tcttcgccgc gtagagcgcg
     4141 tcgcgggccg cgtccacgag cgcttcggcc ggcccctggt agcgcgggga cgggccccgc
     4201 aggaccgcgc tcgcggccac ccgctcaacc ttcagcgccg agatgagccg cccttccacg
     4261 gcggcgttga tcgtggggat cggcgcgccg aggtcgaggg cgttctggct ggtccacttc
     4321 cccgtgccct tctgcgccgc ctcgtcgagg atcacgtcca ccagcgggcg cccggtctcg
     4381 ccgtccaccc tggcgagcac ctgcgcggtg atctcgatca ggtaggagcg caggacgccg
     4441 cggttccact cctcgaagat cgcgctcagc tccgaggcgg ggaggccgag gccgcgctgc
     4501 agcaggtcgt agacctcggc gatcagctgc atgtcgccgt actcgatgcc gttgtgcacc
     4561 atcttcacgt agtgccctgc gccgagcggc cccatgtagt cgacgcacgg ctcgccgtcc
     4621 tcggccttcg ccgcgatcgc gcggagaacg ggcgccagcg cctcccacgc ctcgacctgc
     4681 ccgcccggca tgatgctcgg cccccagagc gcgccctgct cgccgcccga gacgccggtg
     4741 ccgatgtagc ggaagtccga gccggccagg tcgcggttcc gccgctcggt gtcgaggaac
     4801 caggagttgc cgccgtcgat caggatgtca cccggggcga ggtgcggcct gaggtgcgcg
     4861 atcgcgtcgt ccaccgggcg cccggccggc accatcatga gcacgcgccg cggcagctgc
     4921 agcatcgcca tcagcgcggc cggcgactcg gcgcccgcta cccgcttccc tttccccggg
     4981 ccgtcgagga acgccttcgt ctggg
//
LOCUS       DAOPQX010000271         4997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18298_length_4997_cov_3.376771, whole genome shotgun sequence.
ACCESSION   DAOPQX010000271 DAOPQX010000000
VERSION     DAOPQX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18298_length_4997_cov_3.376771"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1276)
                     /locus_tag="PKK95_13000"
     CDS             complement(11..1276)
                     /locus_tag="PKK95_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOC19185.1"
                     /translation="MAKRSCKLALVFLLVLGAVPAVAAPVTVMWDPNPESDIVSYIVH
                     WGTRPGIYTNRTSVDGRTTSWLSPDLRGGRTYYFAVQAVNADGLSSPYSEEASISVPG
                     VWRAVLGDFSGSGTANFSVFRRDRGQWLIRDGTSVAFGNSTDIPVVGDYDGDGLSDIA
                     LWRPSSGVWSVLHSSDGYQFGSMLQVQWGAGRVNDVPVPADYDGDGRTDIAVWRPGPG
                     TWYILLSGSEYDTAQALVVQWGAGRVKDVPVPADYDGDGAADLAVWRAQNGTWYILPS
                     SEGFATSAARSIQWGAGRVKDVPAPGDYDGDGASDLAVWRPGTGVWYILLSSEDFSTG
                     AAMTLNWGAGSRGDVPVPADFDGDGTTDIAVWRPSNGTWYVLQSSQRFASSGALAIQW
                     GSLALHDLPVTGTVPLAATKPALAGYASR"
     gene            complement(1513..1989)
                     /gene="smpB"
                     /locus_tag="PKK95_13005"
     CDS             complement(1513..1989)
                     /gene="smpB"
                     /locus_tag="PKK95_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOC19186.1"
                     /translation="MEKSERQKAQQVIAENRKAFHDYHIVETFEAGLALLGTEVKAIR
                     EGRANLRDSFGRVEGGEVFVHNVHISPYSHRGYATHEPLRKRKLLLHRLEIRKLIGKT
                     AERGMTIVPLRLYFKNGRVKMAIGLARGKKEYDRRETIKRRETERETRAAVKERQR"
     gene            complement(2047..2946)
                     /gene="pdxA"
                     /locus_tag="PKK95_13010"
     CDS             complement(2047..2946)
                     /gene="pdxA"
                     /locus_tag="PKK95_13010"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOC19187.1"
                     /translation="MTPSSSALPRIALTMGDPCGIGPEIVEQAAADPRVRDACEPVIY
                     GPADLSPFTPGRVAAAAGRAAYEAIVRAVEDARAGRVAALATAPVSKEAFALAGLPWR
                     GHTDLLAHLTGATRYAMMFYSDRLRVVLATVHVALAEVPRLITRASIRGLIDLTAAEL
                     PRFGFPRPHLAVAALDPHAGEHGVIGGQDDEVLRPAVEEARASGIDVEGPFPADTLFV
                     RAARGDFDAIIACYHDQGLIPVKLLAFGRSVNVTIGLPIVRTSVDHGTAFDIAGKGVA
                     DPGSLIEAVLLAARLAARPNPST"
     gene            complement(3005..3736)
                     /gene="ricT"
                     /locus_tag="PKK95_13015"
     CDS             complement(3005..3736)
                     /gene="ricT"
                     /locus_tag="PKK95_13015"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOC19188.1"
                     /translation="MEQNPEHPGVRVRLCRSGRPCTFSVTPAQAATPLRSGLPVVVET
                     DLGLSFGHIVGTPVCRGAQPPAEKRVVRLATEDDVAMRRRQRQRELEAFRVALLKIRE
                     RSLGMKLTRVEQLYDGSRLIFYFTAEGRVDFRELVRELAAEFRCRIEMRQIGVRDEAR
                     MLGGYGSCGRPLCCASWLQAFEPVSIKMAKQQDLSLNPSKLSGLCGRLKCCLRYELPE
                     GKLPRRAETRDEALANGENGGNSGS"
     gene            complement(3745..4749)
                     /locus_tag="PKK95_13020"
     CDS             complement(3745..4749)
                     /locus_tag="PKK95_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOC19189.1"
                     /translation="MPFSDIIGHARPLGLLSRSIALGTLPPSLLLTGPAGVGKRYAAV
                     AVAQALNCLAPVREGRFPIDACGCCSPCRRIAKLSHPDFLVLEPGDSGNIKIDPVREA
                     LEATAYLPFEARARVVVVDEAERVLPEAQNALLKSLEEPPRATFFALVTSQPETLLPT
                     IRSRCSRVRFGRLTPAEVANLLIGREKMDSSEAHAAAAVAEGSPGRALERRTRGFRAA
                     REAALGALKASAGRSWQDRMRAAAALAPKTGSGERDELATRIRLLATLLRDAALVGQH
                     GGGTALANADLAGEIERIAPAFDAGRATRAFEAADFALAALRRNASPKIVAPWLVVHL
                     "
     gene            complement(4792..>4997)
                     /locus_tag="PKK95_13025"
     CDS             complement(4792..>4997)
                     /locus_tag="PKK95_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19190.1"
                     /translation="GRLLGVFKGGDDGKKNEKKKQDEKKRQDEKEQDQGKKQDGHQKQ
                     EEASGEADTEKDEVDRGSGGGRI"
BASE COUNT          744 a         1827 c         1679 g          747 t
ORIGIN      
        1 cagtccggct tcagcggctc gcgtagcctg caagggccgg cttggtggcc gcgagtggca
       61 ctgtgcccgt caccgggaga tcgtgcaacg ccagcgaacc ccattgaatc gccagcgcgc
      121 ccgaggatgc aaaccgctgg ctcgactgga gcacgtacca tgtgccgttg gatgggcgcc
      181 agaccgcgat gtcggtggtg ccgtccccgt cgaagtcggc cggcaccggg acgtcgcccc
      241 ggcttcccgc gccccagttc agcgtcatgg cagcacccgt gctgaaatcc tcgctcgaga
      301 gaaggatgta ccagacaccg gttcccggcc gccagaccgc caggtcgctc gcaccgtcgc
      361 cgtcgtagtc accgggagcc ggcacgtcct tcacgcggcc ggcgccccac tggatcgaac
      421 gggcggcgct ggtcgcgaag ccctcgcttg atggaaggat gtaccaggtg ccgttttgcg
      481 cccgccagac cgcaaggtcc gccgcgccgt cgccgtcgta gtcggccggc accggcacgt
      541 ccttcacgcg gccggcgccc cactgcacga cgagggcctg cgcggtgtcg tactcgcttc
      601 ccgagagcag gatgtaccac gtgcccggac ccggccgcca caccgcgatg tcggtccgcc
      661 cgtcgccatc gtagtccgcg ggcaccggca cgtcgttcac gcgcccggcg ccccattgca
      721 cttgcagcat cgaaccgaac tggtagccgt cgctcgaatg caggaccgac caaacgccgc
      781 tcgacggccg ccacagcgcg atgtcgctga ggccatcacc gtcgtagtcg ccgacaaccg
      841 ggatgtcggt cgagttcccg aaggcaaccg atgtgccgtc gcggatcagc cactgcccac
      901 ggtcgcggcg gaacacggag aaattcgccg tgccgctgcc cgagaagtcg cccaacaccg
      961 cccgccagac cccggggacg ctgatggagg cctcctccga atatgggctc gacagcccgt
     1021 ccgcgttcac agcctgcacc gcgaagtaat acgtccgccc gccgcggagg tcaggcgaca
     1081 accaggaggt cgttctccca tcgaccgagg ttctgttggt gtagatcccc gggcgggtcc
     1141 cccaatgcac gatgtacgac acgatgtccg actccgggtt ggggtcccac atcaccgtga
     1201 ctggggccgc cacggccgga accgccccga gcaccaggag gaagaccagg gcaagtttgc
     1261 agcttctttt cgccatgatg ccttcgggcg agggaatctc gcgtgatcct gggttcgcaa
     1321 gcgcggtgcc cagccgtttc cccaggcggc gcgatacgca tgagctgaaa tgctcaacaa
     1381 tttggcgact cggagctgcc acggtcgcac acggccagcg gccggattac gaatccgtaa
     1441 cgccaaattc ggacttgttc gggtgatggt cgcgccggcg gagggaagga accgcaggca
     1501 gcgcgggggc cgctatcgct gccgctcctt cacggccgcg cgggtctcgc gctcggtctc
     1561 gcgccgcttg atcgtctcgc gccggtcgta ctccttcttg ccgcgggcca ggccgatggc
     1621 catcttcacg cggccgttct tgaagtagag gcgtagcggg acgatcgtca tgccgcgctc
     1681 ggcggtcttc ccgatcagct tgcggatctc cagccggtgc agcagcagct tgcgcttccg
     1741 cagcggctcg tgggtggcgt agccacggtg gctgtagggg ctgatgtgca cgttgtgcac
     1801 gaacacctcg ccgccctcga cccgcccgaa gctgtcgcgc aggttggcgc gcccctcgcg
     1861 gatcgccttc acctcggtgc cgagcagcgc caggcccgcc tcgaaggtct cgacgatgtg
     1921 gtagtcgtgg aaggccttgc ggttttcagc gatgacctgc tgcgccttct ggcgctcgga
     1981 cttctccacc atgccaaccc caatcattca gtctacacgg gcttcgggcc gggcgcgccg
     2041 cccgtgtcag gtggagggat tgggacgcgc agcgagccgc gcggccagca gcaccgcctc
     2101 gatcaggctg cccgggtcgg ccacgccctt ccccgcgatg tcgaacgccg tgccgtgatc
     2161 gaccgacgtg cggacgatgg ggaggccgat cgtcacgttc accgagcggc cgaacgccag
     2221 cagcttcacc gggatcagcc cctggtcgtg gtagcaggcg atgatggcgt cgaagtcgcc
     2281 gcgcgccgca cgcacgaaga gcgtgtcggc ggggaatggg ccctcgacgt cgatgccgga
     2341 ggcgcgcgcc tcctccaccg cgggccgcag cacctcgtcg tcctgacccc cgatcacccc
     2401 atgctcgccg gcgtgcgggt cgagcgccgc gaccgcaagg tgcggtcgcg gaaagccaaa
     2461 gcggggcagc tccgcggcgg tgagatcgat caggccgcga atcgaggcgc gcgtgatcag
     2521 ccgaggcacc tcggccagcg cgacgtgcac ggtggccagc acgacgcgca ggcgatcgga
     2581 gtagaacatc atcgcgtagc gcgtcgcgcc ggtgaggtgc gcgagcaggt cggtgtggcc
     2641 gcgccagggc aggccggcca gcgcgaacgc ctccttgctc accggcgcgg tcgccaacgc
     2701 cgccacgcgc ccggcgcgcg cgtcctccac cgcgcggacg attgcctcgt acgcggcgcg
     2761 cccggccgcc gcggccaccc gccccggcgt gaagggggaa agatcggcgg gaccgtagat
     2821 cacgggctcg caggcgtcgc gcacgcgcgg gtcggccgcc gcctgctcga cgatctcagg
     2881 accgatcccg cacggatcgc ccatcgtcag ggcgatgcgc ggaagggcgg atgatgaggg
     2941 agtcacaatc caggcgccag gcaaccacag ggccggccca cgcgggcgac cacaagggtc
     3001 gcccctacga tccgctattc cccccattct ctccgttcgc gagcgcctcg tcgcgcgtct
     3061 ccgcgcggcg cggcagcttc ccttcgggga gctcgtagcg gagacagcac ttcagccgac
     3121 cgcacagccc cgacagcttc gacgggttca ggctgaggtc ctgctgcttc gccatcttga
     3181 tcgagaccgg ctcgaacgcc tggagccagg aggcgcagca gagcggtctc ccgcacgatc
     3241 cgtagccgcc gagcatccgg gcctcgtcgc ggacgccaat ctgccgcatc tcgatgcggc
     3301 agcggaactc ggccgccagc tcgcgcacca gctcccggaa gtccacccgc ccttccgccg
     3361 tgaagtagaa gatgaggcgc gagccgtcgt acagctgctc gacgcgcgtc agcttcatgc
     3421 cgagcgagcg ctcgcggatc ttcagcaacg ccacgcggaa cgcctccagc tcgcgctggc
     3481 gctgccggcg ccgcatcgcc acgtcgtcct cggtggcgag gcgcaccacc cgcttctcgg
     3541 ccgggggctg cgcgccgcgg cacaccggcg tgccgacgat gtggccgaag gagaggccca
     3601 gatcggtctc gaccaccacc gggagccccg agcggagggg cgtggcggcc tgcgccggcg
     3661 tcaccgagaa ggtgcaggga cgcccgctcc tgcacagcct cacgcgcacg cccgggtgtt
     3721 cggggttctg ttccatgggc ccgctcacag gtggacgacc agccacgggg cgacgatctt
     3781 cgggctcgcg ttgcggcgga gcgccgccag cgcgaagtcg gccgcctcga acgctcgcgt
     3841 cgcacgcccg gcgtcgaagg ctggagcgat ccgctcgatc tccccggcca gatcggcatt
     3901 cgccagcgcc gtgcccccgc cgtgctgccc cacgagcgcg gcatcgcgga gcaaggtggc
     3961 gagcaggcgg attctcgtcg ccagctcgtc gcgctcgccc gagcccgtct tgggcgccag
     4021 tgccgcggcc gcccgcatcc tgtcctgcca cgaccggccg gccgaggcct tcaacgcgcc
     4081 cagagccgcc tcgcgcgccg cgcggaaccc tcgggtgcgg cgctcgagcg cgcgacccgg
     4141 gctcccctcg gcgaccgccg ccgccgcatg ggcttcggat gaatccatct tctcgcgccc
     4201 gatcagcagg ttcgccacct cggccggggt caaccggccg aagcgtaccc gtgagcagcg
     4261 cgagcggatc gtgggcagca gggtctcagg ctgagaggtg accagggcaa agaaggtggc
     4321 gcgcggcggt tcctccaggc tcttcagcag cgcgttctgc gcctcgggca gcacccgctc
     4381 ggcttcgtcc accaccacca cgcgagcgcg cgcctcgaag gggaggtacg ccgtcgcctc
     4441 gagggcctcg cgaaccgggt cgatcttgat gttgcccgag tcccccggct cgagcaccag
     4501 gaaatcggga tgggagagct tggcgatgcg gcggcagggg ctgcagcagc cgcaggcgtc
     4561 gatcggaaac cggccttctc gcaccggcgc gaggcagttc aaggcctgcg ccacggccac
     4621 cgccgcgtag cgcttcccca ccccggcggg gccggtcagg aggaggctgg gcggcagggt
     4681 tccgagtgca atggacctgg agagcaggcc cagaggacga gcatgtccga tgatgtccga
     4741 gaacggcatg cgccgaccag cggatactac cacgccggcc ggccagtcat ctcagatcct
     4801 gccgccgccg ctgcccctgt ctacctcgtc cttttccgtg tcggcctccc cggacgcttc
     4861 ttcctgcttc tgatggccgt cctgtttctt cccctggtct tgctccttct cgtcctgccg
     4921 cttcttctcg tcttgcttct tcttctcgtt cttcttcccg tcgtcgccgc ccttgaagac
     4981 gccgagcagg cggcccc
//
LOCUS       DAOPQX010000272         4993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18319_length_4993_cov_4.603686, whole genome shotgun sequence.
ACCESSION   DAOPQX010000272 DAOPQX010000000
VERSION     DAOPQX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18319_length_4993_cov_4.603686"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..2605)
                     /locus_tag="PKK95_13030"
     CDS             complement(107..2605)
                     /locus_tag="PKK95_13030"
                     /inference="COORDINATES: protein motif:HMM:NF023454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HOC19191.1"
                     /translation="MSPSLGDLLPETIDPSPGELLDRFLEYTGGKGLTLYPAQEEAIL
                     ELLEGKNVILNTPTGSGKSLVASAMLFAAVARERRAVYTCPIKALVNEKWMALCRDFG
                     PERVGLSTGDATVNRDAPILCCTAEVLANISLREGEGALVDDVVMDEFHWYADRDRGV
                     AWQVPLLTLPYARFLLMSATLGDTSFFESALTRLTDRPTVSVRSEDRPVPLEFAYAEI
                     PLAHTLEKLADEGKTPVYVVHFTQAEAASSAQDFTSLKICTREEKQAIAETIADFRFT
                     SPYGSEIRKWLRHGIGLHHAGLLPKYRVLVEQLAQRGLLKIICGTDTLGVGINVPIRT
                     VLFTRLCKFDGHKTAHLSARDFHQISGRAGRRGFDTKGFVVVQAPEHVIENKRLSEKA
                     ARDGKKVVKRRPPEHNFVNWDLGTFKRLIGSAPEQLASRFQVSHGMLLNVLSRRGDGC
                     AAMRQIVRDSHETETAKQAHRKRAWQLFRALVAKGIVEIVPRTEDGAKVRVNVELQDD
                     FSMDQELSLYLLEAIPLLEAGSPTYALDVLTLVESILENPEVILRRQLDKIKDRAVAQ
                     MKADGVEYEKRMEELEKLEYPKPLRDFVYDTFNAFADRHPWVGEENIRPKSIAREMFE
                     GFRSFAEYVMDYDLERVEGLLLRHLNSVYKVLSQTVPDSAKTDEVLEMEEYFRTMVRG
                     VDSSLAEEWERMRAGEGWVPGAVPAVEAPDQRPTGVEAADAARAARAFTAAVRTKVFA
                     FLRNWERGGDAEAFRAEHGSLRFDPEGRNARHTTITPSEDGATWRVQQMLVDTEGHND
                     WVAEFDVDLAASRDAPEPALTLRWIGPLEGAPAA"
     gene            2736..3371
                     /locus_tag="PKK95_13035"
     CDS             2736..3371
                     /locus_tag="PKK95_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC19192.1"
                     /translation="MESSEPAAQAPAGRVDRRKQRTRDAVVAAAMRLFRRHGFDGVTM
                     EQIAEAADIAKGTLYSYFPVKEAIVAAYLDKESLVRNAERLVRLRALGGTRARLAASL
                     GELIEAVRAQPDIFEKYFTYRTRQMISLRRERNQVSGLRLLEDEIIRLGQDAGELRRD
                     LPFELLEALFEFCFIILAQRYYEQREAFDLSRAVGQCVELFMNGAGKERAR"
     gene            3371..4315
                     /locus_tag="PKK95_13040"
     CDS             3371..4315
                     /locus_tag="PKK95_13040"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOC19193.1"
                     /translation="MSGPSLKSRALIFAVRHRHLFQGRLRPEVITTETNTSELRRRFE
                     RGAQRMGTVPEGIDVVPVRIDGMQAEWIQPSGAAREEAVLYTHGGGYVTGSCADHRVH
                     VAKLVGASGVAALVFEYRLAPEYPAPAAVEDTLAAYRWLLATGIPAKRIAVAGESAGG
                     GLGLASLLAMRDRGLPMPAAGVASSPWLDLTCTADSYRRNARKDISILGSWEVWSRYY
                     VGAGDPRHPWISPLYGDLRGLPPLLLQVGTREIMLDDTRRFAELARAAGVEVTLRVWD
                     GMVHCFAFFAPMFPEATAGMRELGGFIRERLAGTAAGA"
     gene            4335..4928
                     /gene="pdxH"
                     /locus_tag="PKK95_13045"
     CDS             4335..4928
                     /gene="pdxH"
                     /locus_tag="PKK95_13045"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOC19194.1"
                     /translation="MPLASEAPADPIEIFRALYERASRKCVDADAMVLATVDPDGRPS
                     ARYVLLKGFDARGFVFFTNIESRKGRALAARPDAALCFYWAPLERQVRIEGRAELVSD
                     AEADAYFETRPRESQIGAWASAQSEPLDSPALLEQRAAEVRARFAGRPVPRPPFWSGY
                     RVVPRAIEFWTRGAGRLNERERYEREGGGWVRTFLYP"
BASE COUNT          715 a         1708 c         1769 g          801 t
ORIGIN      
        1 tgagcccccc ttcgcctgct cgctggggct cgcggctacg gcgggcaagc ccgagccttg
       61 cgccttgagc cctgagccct gagccgtgag ccttgagccc tgagccctac gccgccggcg
      121 ccccttcgag cgggccgatc caccgcaggg tcagcgccgg ttccggcgcg tcgcgcgagg
      181 cggccaggtc gacgtcgaac tcggcgaccc agtcgttgtg accctcggtg tccaccagca
      241 tctgctggac ccgccacgtg gcgccgtcct ccgaaggggt gatggtcgtg tggcgggcgt
      301 tgcgcccttc cgggtcgaag cgcagcgagc cgtgctcggc gcggaaggcc tccgcgtcgc
      361 ctccccgttc ccagttgcgg aggaaggcga agaccttcgt gcggacggcg gcggtgaagg
      421 cgcgtgcggc tcgtgcggcg tcggccgcct cgacgcccgt cggccgctgg tccggcgctt
      481 ccaccgccgg aacggccccg gggacccatc cctcgcccgc acgcatccgt tcccactcct
      541 cggccaggct cgagtccacg ccgcgcacca tcgtccggaa gtactcctcc atctcgagca
      601 cttcgtcggt cttggccgag tcggggacgg tctggctgag caccttgtag acgctgttca
      661 ggtgccgcag cagcagcccc tccacccgct cgaggtcgta gtccatgacg tactcggcga
      721 acgagcggaa gccctcgaac atctcgcgcg cgatcgactt cgggcggatg ttctcctcgc
      781 caacccaggg gtggcggtcg gcgaaggcgt tgaaggtgtc gtagacgaag tcgcgcagcg
      841 gcttcgggta ctccagcttc tccagttcct ccatccgctt ctcgtactcc acgccgtcgg
      901 ccttcatctg cgccaccgcg cggtccttga tcttgtcgag ctggcggcgg aggatgacct
      961 cggggttctc gaggatcgat tcgaccagcg tcaggacgtc gagcgcgtag gtgggggagc
     1021 cggcctcgag cagcgggatc gcctcgagca ggtagagcga cagctcctgg tccatcgaga
     1081 agtcgtcctg cagctcgacg ttcacccgga ccttcgcgcc gtcctcggtc ctcggcacga
     1141 tctcgacgat ccccttggcg accagcgcgc ggaacagctg ccaggcccgc ttgcggtgcg
     1201 cctgcttcgc ggtctcggtt tcgtgcgagt cgcggacgat ctgccgcatc gcggcgcacc
     1261 cgtcgccgcg ccggctcagg acgttgagca gcatgccgtg cgacacctgg aagcgcgagg
     1321 ccagctgctc gggggccgac ccgatcagcc gcttgaaggt gccgaggtcc cagttcacga
     1381 agttgtgctc gggcggccgc cgcttgacca ccttcttccc gtcgcgcgcg gccttctcgc
     1441 tcagccgctt gttctcgatc acgtgctcgg gcgcctgcac caccacgaac cccttggtgt
     1501 cgaagccgcg ccggcccgcc cgccccgaga tctggtggaa gtcgcgcgcg ctcaggtgcg
     1561 ccgtcttgtg cccgtcgaac ttgcagaggc gcgtgaagag gacggtccgg atggggacgt
     1621 tgatgccgac gcccagcgtg tcggtgccgc agatgatctt cagcagcccc cgctgcgcga
     1681 gctgctccac cagcacgcgg tacttgggga gcagccccgc gtggtgcagg ccgatcccgt
     1741 gccgcagcca cttgcggatc tccgacccgt aggggctcgt gaagcggaag tcggcgatcg
     1801 tctcggcgat cgcctgcttc tcctcccggg tgcagatctt caggctggtg aagtcctgcg
     1861 cgctcgacgc cgcctcggcc tgggtgaagt ggacgacgta gacgggcgtc ttcccctcgt
     1921 cggccagctt ctcgagcgtg tgcgcgagcg ggatctccgc gtaggcgaac tccagcggga
     1981 ccggccgatc ctccgatcgc accgagacgg tgggacgatc ggtcagccgc gtgagcgccg
     2041 actcgaaaaa cgaggtgtca ccaagggtgg ccgacatcag gaggaaccgc gcgtagggca
     2101 gggtgagcag cggcacctgc cacgccacgc cccggtcgcg gtcggcgtac cagtggaact
     2161 cgtccatcac cacgtcgtcc accagcgccc cctcgccctc gcgcagcgag atgttcgcca
     2221 gtacctcggc cgtgcagcac aggatcggcg cgtcgcggtt gaccgtagcg tcgcccgtcg
     2281 agaggccgac gcgctcgggg ccgaaatcgc ggcagagcgc catccatttc tcgttgacga
     2341 gcgccttgat cgggcaggtg tagacggccc gccgctcccg agcgaccgcg gcgaagagca
     2401 tcgccgaggc caccagcgac tttcccgagc cggtgggcgt gttgaggatg acgttcttcc
     2461 cctcgagcag ctcgaggatc gcctcctcct gggcggggta gagcgtcagc cccttgcccc
     2521 ccgtgtattc caggaagcgg tcgagcaact cgccgggaga cgggtcgatg gtttcgggaa
     2581 ggagatcgcc gagcgaaggt gacatcgtgc ccagttgtac cgcgaaaagg ggtcccggcg
     2641 cgacccgctg cgctttcccc ttgcacccgg ccagctcctc ggctactatt gaacgtcgtt
     2701 caatcgtgaa cgtcgtctag tagacggagc cgatcatgga gagcagcgag ccggcggcgc
     2761 aggcgccggc cgggcgcgtg gaccgcagga agcagagaac gcgcgatgcg gtcgtcgcgg
     2821 ccgcgatgcg cctgttcagg cgccacggct tcgacggcgt gacgatggag cagattgccg
     2881 aggcggcgga tatcgccaag ggcactctgt acagctactt cccagtcaag gaagccatcg
     2941 tcgccgccta cctcgacaag gaatcgctcg tccgcaacgc cgagcggctc gtccggcttc
     3001 gcgcgctcgg cggcacccgg gcccgtctcg cggcaagcct cggcgagttg atcgaggcgg
     3061 tgagggcgca gcccgacatc ttcgagaagt acttcaccta tcgcacccgc cagatgatct
     3121 cgctgcggcg cgaacgaaac caggtcagcg gcctgcggct gctcgaggac gagatcatcc
     3181 ggctcgggca ggacgcgggc gagctgcgcc gcgacctgcc gttcgagctg ctcgaggcgc
     3241 tcttcgagtt ctgcttcatc atcctcgcgc agcggtacta cgagcagcgg gaggcgttcg
     3301 acctctcgcg cgcggtcggg cagtgcgtcg agctgttcat gaacggcgcc ggcaaggaaa
     3361 gggcacggtg atgagcggac caagcctgaa gagccgggcg ttgatcttcg ccgtcaggca
     3421 tcgccacctg ttccagggcc gcctgaggcc cgaagtgatc acgacggaaa ccaacactag
     3481 cgagctgagg cggcgattcg agcggggcgc ccagcggatg gggacggtgc cggaggggat
     3541 tgacgtcgtt ccggtgcgga ttgacgggat gcaggccgag tggatccagc cctcaggtgc
     3601 ggcgcgcgaa gaggccgtcc tctacacgca cggcggcggc tacgtgacgg gcagctgcgc
     3661 cgaccaccgc gtgcacgtgg cgaagctggt gggggcgtca ggcgtcgccg cgctcgtgtt
     3721 cgagtaccgg ctggcccccg agtatccggc gcccgccgcg gtggaagaca cgctcgccgc
     3781 gtaccgctgg ctgctggcga ccggtatacc cgcgaagagg atcgcggtgg cgggcgagtc
     3841 ggccggggga gggctgggcc tggcgtcgct gctggcgatg cgcgaccggg ggctgccgat
     3901 gcccgcggcc ggcgtcgcct cgtcgccgtg gctcgacctg acgtgcaccg ccgattcgta
     3961 ccgccgcaac gcgcggaagg acatctcgat cctggggtcg tgggaagtat ggagccggta
     4021 ctacgtcgga gcgggcgacc cgcggcaccc gtggatctcg cccctctacg gcgacctgcg
     4081 cggcctgcct ccgctcctcc tccaggtcgg cacgcgcgag atcatgctcg acgacacgag
     4141 gcggttcgca gagctggcgc gtgcggcggg cgtcgaggtc acgctccggg tctgggacgg
     4201 gatggtgcac tgctttgcat tcttcgcccc gatgtttccc gaggcgacgg cgggaatgcg
     4261 cgagctgggc ggcttcattc gagagcggct ggcggggacg gcggccggcg cctgaggccg
     4321 catatacttc gttcatgccc ctggcaagcg aggcgcccgc cgatccgatc gagatctttc
     4381 gcgcgctcta cgagcgcgcc agccggaagt gcgtggacgc cgacgcgatg gtgctggcga
     4441 cggtcgatcc cgacggccgg ccttccgccc gttacgtgct gctgaagggg ttcgacgccc
     4501 gcggcttcgt cttcttcacg aacatcgaga gccgcaaggg gcgcgcgctg gccgcgcgtc
     4561 ccgatgcggc cctttgcttc tactgggcgc cgctcgagag gcaggtacgg attgaagggc
     4621 gcgccgagct cgtctccgac gcggaggccg acgcgtactt cgagacgcgg ccgcgcgagt
     4681 cgcagatcgg ggcgtgggcc tcggcgcaga gcgagcctct cgactcgccg gcgctgctcg
     4741 agcagcgggc cgccgaagtg cgggcccgct tcgccggccg gccggtgccg cggccgccct
     4801 tctggtcggg ctacagggtc gtgcctcgcg caatcgagtt ctggacccgc ggcgccgggc
     4861 gcctgaacga gcgcgagcgg tacgagcgcg agggcggcgg ctgggtgagg acgtttctct
     4921 acccgtgagt tctcattcgc acaggcggga cgccagtgcg tgccagtggc acaggcgtct
     4981 cgcctgtggg cgg
//
LOCUS       DAOPQX010000273         4971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18446_length_4971_cov_3.822823, whole genome shotgun sequence.
ACCESSION   DAOPQX010000273 DAOPQX010000000
VERSION     DAOPQX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18446_length_4971_cov_3.822823"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="PKK95_13050"
     CDS             complement(<1..1755)
                     /locus_tag="PKK95_13050"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOC19195.1"
                     /translation="MSRPFVSGLRGRLLLLIVLATLPAFALTFLTGWRDRQRQRAEVA
                     AGTMGLARQIAADQERIIDGTRQILFDLAQVPEVREGDPARSRTFFAILMKQYRGYAS
                     FAVVAPNGDVLASLPRSAEPVNFADRDWFREAVGERRFAVGDYQVGQLTKTAVLVAAW
                     PAITADGTLTSVITAALDVRWLNEIAAASQLPPGGLLLLVDRRGQVIARHPEGEGWIG
                     RHLPNPSLSAVMKDQAEGVVEAADPDTLERIYAFTPIRGRANTELRVVIGVPRETAYA
                     AVNQLQTRQLIMLALVIALTLAGAWVFAERWVLRRVASLLDATRKLAAGDRSVRTRLP
                     YGHTELGELARAFDEMAGALETRQAERDRAEQALRASDARFRAFMAHSPAIAFIKDEA
                     GRYEYANAAFERFLGIEPDGWAGRTDEDFFSPEAARALSLATQALGDGSGPRQTLQAV
                     RRRDGTEASWLLVTFPLGDPASPKLAGMAVDLTDSQQMREALRRSEQQYATLVEQAAD
                     GIVITDAGRRLVAVNSAVCAMSGYSPDELIGRPVDDLFAPGSLAALLDRTKGDGLAGA
                     VPEIEAVRKDGGRFPAEVS"
     gene            2096..2905
                     /locus_tag="PKK95_13055"
     CDS             2096..2905
                     /locus_tag="PKK95_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC19196.1"
                     /translation="MLPLHEGLVYGPVRSRRLGRSLGLNILPRQQKVCTFNCSYCQYG
                     WTAVTVDPGTSPGGPWPSPAAIARAAARALAALAARHEPIDRLTVAGNGEPTLHPELP
                     AVIAALREARDEVVPRLPIAILSNASTLGLPGVSAALETLDERYMKLDAGDPALLRRL
                     NAARVPLESIVSGLAGLRDVVIQAMFVKDRTGRFDNATDLAVSAWIVQLLAARPRLVH
                     VYSVDRPTAWPYLQAVPLERLEEIANCVRAAGLEATAFGTPAHGAARRAAG"
     gene            complement(2923..3387)
                     /locus_tag="PKK95_13060"
     CDS             complement(2923..3387)
                     /locus_tag="PKK95_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19197.1"
                     /translation="MSVVPTVLEAAPPLIAPPLEVGVNGPEGCALSPADLRRLFRGRR
                     LTPARRERLAASGRVVARCGSRIVGLAAFEPLGAEVRVHEFGADTGSVVPAERIADVL
                     LDALEVACLACGARRLVLLPRAVVMPGLLQSRGFAVMAEGCAGSWFEKSFPS"
     gene            complement(3522..4493)
                     /locus_tag="PKK95_13065"
     CDS             complement(3522..4493)
                     /locus_tag="PKK95_13065"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC19198.1"
                     /translation="MGRSARMRAVFDFVRVVADSDSSVLVTGETGTGKELIANLIHQS
                     SSRRHKPFVPVSCAILSETLIESELFGHERGAFTGAIKERPGRFELAQGGTIFLDDID
                     DVPLSMQVKLLRVLQNRVVERLGGTRQIPVDVRVVTGSKRDLRQMVAAGKFRDDLFYR
                     LNVIPVNLPPLRDRREDIPILVDHFVKRYFRQRGEEPRAVSPAVLQAFLRYPWPGNVR
                     ELENACERIAQTCTCDTVRLGCVPVSVLFHKYAEDHQPVVENHAHPSAVSLDDRLREV
                     ESNLITWALKVSGGNKSKAAELLSIKRSTLGDRIKKLELTHLETTEQ"
     gene            4667..4891
                     /locus_tag="PKK95_13070"
     CDS             4667..4891
                     /locus_tag="PKK95_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOC19199.1"
                     /translation="MIARERVQTVIDRIRPLIQGDGGDIELVDVIENKAKVRLTGNCV
                     GCPSAQMTLFMGVEMALKEEIPEFEELIVV"
BASE COUNT          769 a         1755 c         1722 g          725 t
ORIGIN      
        1 gctgacctcg gccgggaagc gccctccgtc cttccgcacc gcctcgatct cgggaaccgc
       61 gccggccagg ccgtccccct tcgtacggtc cagcaacgcg gccaggctgc ccggcgcgaa
      121 caaatcgtcc accgggcgtc cgatcagctc gtcgggcgag tagcccgaca tcgcgcaaac
      181 cgccgaattg acagccacca ggcgcctgcc ggcgtcggtg atgacgatcc cgtcggctgc
      241 ctgctcgacc agggtggcgt actgctgctc gctgcgccgc agcgcctcgc gcatctgctg
      301 cgagtcggtg agatcgaccg ccatgcccgc gagcttcggc gacgccgggt cgccgagcgg
      361 gaaggtgacc agcagccacg acgcctcggt tccatcacgg cggcgaacgg cctgcagcgt
      421 ttgccgcggg cccgagccgt cgcccagggc ctgggtcgcg aggctcagcg cacgggcagc
      481 ctccggggaa aagaagtcct cgtcggtgcg cccggcccac ccgtccggct cgatgccgag
      541 aaagcgctcg aaggcggcat tggcgtattc gtaccgcccg gcctcgtcct tgatgaaggc
      601 gatggcgggg ctgtgagcca tgaacgcccg gaagcgcgcg tcactcgccc ggagggcctg
      661 ctcggcgcga tcgcgctcgg cctgtcgcgt ctcgagggcg ccggccatct cgtcgaacgc
      721 gcgcgccagt tcgcccagtt cggtgtggcc gtacgggagg cgcgtccgca cagagcggtc
      781 gccggcggcg agtttgcgcg tcgcgtcgag aagggaggcc acgcggcgca ggacccagcg
      841 ctcggcgaac acccaggccc cggccagggt gagggcgatg acgagcgcca gcatgatcag
      901 ctgccgcgtc tgcagctggt tgacggcggc gtacgcggtt tcgcgcggca cgccgatgac
      961 cacgcgcagc tcggtgttcg cccggccgcg aatcggcgtg aacgcgtaga tgcgctcgag
     1021 cgtgtccggg tcggcggctt caaccacgcc ctcggcctgg tctttcatga cggccgagag
     1081 cgacgggttc ggcaggtggc ggccgatcca tccctcaccc tcggggtggc gggcgatcac
     1141 ctggccccgg cggtccacca gcagcagcaa gccacccggg ggcagctggc tggcggcggc
     1201 gatctcgttc agccaccgca cgtcgagggc cgcggtgatc accgacgtga gggtgccgtc
     1261 ggcggtgatg gccggccagg cggccaccag cacggcggtc ttggtcagct ggccgacctg
     1321 gtagtcgccc acggcgaagc ggcgctcgcc aacggcctcg cggaaccagt cacggtcggc
     1381 gaagttgacg ggctcggccg acctcggaag gctggccagc acgtcgccgt tcggggccac
     1441 cacggcgaaa ctggcgtagc cgcggtactg cttcatcagg atcgcgaaga aggtgcggct
     1501 gcgcgccggg tcgccctcgc ggacctcggg cacctgcgcg agatcgaaga ggatctggcg
     1561 cgtcccgtcg atgatgcgct cctggtcggc ggcgatctgg cgggcgaggc ccatcgtccc
     1621 ggccgccacc tcggcacgct ggcgctgccg gtcgcgccac ccggtcagga aggtgagcgc
     1681 gaaggccggc agcgtggcca ggacgatcag caacagcaac cggccgcgaa gtccggacac
     1741 gaagggacgt gacacgtgtg ctcccgggcg gaaccagcgc cctggcgggt gacctcgggt
     1801 ttcgagcgta gagagcgaaa ggcgatcgcg gtcggcgtca gagcggagcg gacgagggaa
     1861 acggagaagg cggcgcctcg ccccgtccac cgctgatccg ccctgcggtt cgccgctgat
     1921 tgtggccgcg cgcgggtgga aaatcaagcg cccgggctgg cctcgaactt gtaacccttg
     1981 ctccctgcgc gggatccggc cccgactcct tcccgatttc tgaacgggtg ccgcggggcg
     2041 ctcgcgggaa tccggcgctt cttcttccga aaacccggca tctcaggggt ttcccatgtt
     2101 gccgctccac gagggcctcg tctacggccc cgttcgttcg cgcaggctcg gccggtcgct
     2161 cggactcaac atcctccccc gccagcagaa ggtctgcacg ttcaactgct cttactgcca
     2221 atacggctgg accgccgtga ccgttgaccc gggaacctcc ccgggcggcc cctggccctc
     2281 accagcggcc atcgcccggg ccgcggcgcg cgccctcgcc gcgctcgccg ccaggcacga
     2341 gccgatcgac cgcctcacgg tggccggcaa cggcgagccg acgctgcacc cggagctgcc
     2401 ggcggtgatc gcggcgctcc gcgaggcgcg cgacgaggtg gtgccccggc tgccgatcgc
     2461 catactgtcg aacgcgagca cgctgggact gcccggcgtc agtgccgcgc tcgagaccct
     2521 cgacgagcgg tacatgaagc tcgacgcggg cgacccggcg ctgctgcgcc ggctcaacgc
     2581 cgcgcgcgtg ccgctcgagt cgatcgtctc gggcctcgcc ggcctgcgcg acgtcgtcat
     2641 ccaggccatg ttcgtgaaag atcgcaccgg ccgtttcgac aatgcgaccg acctcgcggt
     2701 ctcggcgtgg atcgtccagc tgctggcggc gcggccgcgc ctcgtgcacg tctactcggt
     2761 ggatcgcccg accgcgtggc cctacctgca ggcggtgccg ctcgagcgac tggaggagat
     2821 cgcgaactgc gtgcgggcag ccgggctcga ggccaccgcg ttcggaacgc cggcgcacgg
     2881 ggcggcgcgg cgcgccgcag ggtgagcgga agttgagcag cgtcaggagg gaaacgactt
     2941 ctcgaaccag ctgccggcgc atccttcggc catcaccgca aagccgcggc tttgcagcag
     3001 accgggcatg acgaccgccc gcggaagcag gacgagccgg cgggccccgc atgccaggca
     3061 cgcgacctcg agggcatcca gcagcacgtc cgcaatccgc tcggcaggca ccaccgaacc
     3121 ggtgtcggcc ccgaactcgt ggacgcgaac ctccgccccc agcggctcga aggccgccag
     3181 cccgacgatc cgcgaaccgc accgcgcgac gacacgcccg ctggccgcca gccgttcgcg
     3241 gcgggccggc gtcaggcggc ggccgcggaa caggcgccgc aggtcggcgg gcgacagggc
     3301 gcacccctct ggaccgttca cccccacctc cagcggcggg gcgatcagcg gcggggcggc
     3361 ttcgagcacg gtgggcacga cagacaaccg agcaactgaa gtgccaacac cgggacggcc
     3421 ggaagtgtca aaaacaggcc tgttttgacg tcatgtcgag ccgagtccgg ttttttcctc
     3481 cgaaaactcg gcgcggcgcg ccggtccctc ggcgctcctt cctactgctc cgtcgtttcg
     3541 aggtgggtga gttcgagctt cttgatgcga tcgccgaggg tggagcgctt gatgctgagc
     3601 agctcggccg ccttcgactt gttgcccccg ctcaccttca gggcccaggt gatcaggttc
     3661 gactcgacct cgcgaaggcg gtcgtcgagc gagaccgccg agggatgggc gtggttctcg
     3721 acgaccggct ggtgatcctc ggcgtacttg tggaacagca cgctgaccgg gacgcacccg
     3781 aggcgaaccg tgtcgcacgt gcaggtctgc gcgatccgtt cgcacgcgtt ctcgagctcg
     3841 cgaacgttcc cgggccacgg gtagcgcagg aaggcctgca gcaccgccgg ggacaccgca
     3901 cgcggctcct cgccccgttg ccggaagtac cgcttcacga agtggtccac caggatcgga
     3961 atgtcctcgc ggcgatcgcg cagcggcggc aggttcaccg ggatgacgtt caggcgatag
     4021 aagaggtcgt cgcggaactt ccctgcggcg accatctgcc tgagatcgcg cttggagccg
     4081 gtcaccacgc gaacgtccac cgggatctgc cgcgtcccgc ccaaccgctc caccacgcgg
     4141 ttctggagca cccgcagcag cttcacctgc atcgacaacg ggacgtcgtc gatgtcgtcg
     4201 aggaagatcg tgcctccctg cgccagctcg aaccggcccg gccgttcctt gatcgcgccg
     4261 gtgaaggcgc cgcgctcgtg cccgaacagc tccgactcga tcagcgtctc ggacaggatc
     4321 gcacagctga cggggacgaa cggcttgtgg cgccgggagc tcgattgatg gatgaggttg
     4381 gcaatcagct ccttcccggt gccggtttcg cccgtcacca ggacgctgct gtcgctgtcg
     4441 gccaccacgc gcacgaagtc gaacaccgca cgcatgcgcg cactgcggcc cacgacgacc
     4501 gagtctatgg tgaatggggc tgccatgtag gggtctggct cattctacca gatgtcgcgg
     4561 ggcggcctcc ccgctatact ggataccagc ggggttcaat cggccgcttc cctttttcca
     4621 tccaccaggt tcgacgagcg gggcgcccag cccagccgga gcgcatatga ttgcacgcga
     4681 acgtgtccag accgtgatcg atcgaatccg tcccctcata cagggcgacg gcggggacat
     4741 cgagctggtc gacgtgatcg agaacaaggc caaggtccgg ctgaccggca actgcgtggg
     4801 ctgccccagc gcacagatga ccctgttcat gggggtcgag atggcgctca aggaggaaat
     4861 ccccgagttc gaagagctca tcgtcgtgtg agacggtgag cgggaaagga caacggtcgc
     4921 ggattcgatg ctgcacgtcc tcgatctcga cttccactcc ggcccccgct c
//
LOCUS       DAOPQX010000274         4968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18459_length_4968_cov_4.304905, whole genome shotgun sequence.
ACCESSION   DAOPQX010000274 DAOPQX010000000
VERSION     DAOPQX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18459_length_4968_cov_4.304905"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKK95_13075"
     CDS             complement(<1..180)
                     /locus_tag="PKK95_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19200.1"
                     /translation="MSLYQPHLMTIEAIQDETPDTRTLRLAFRDADLAAGFSFKAGQF
                     GEYSVFGAGECTFCIA"
     gene            complement(214..1248)
                     /locus_tag="PKK95_13080"
     CDS             complement(214..1248)
                     /locus_tag="PKK95_13080"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC19201.1"
                     /translation="MTVISEADLQGLAADLLAAGSTVWGPARVPGTSRPGVEYRPLRE
                     AAELDLNAGLPLLPLKRVFLPPTEPLFWWERTAAEVRLVPPAPPSGRVVVIGARPCDA
                     AGADAMDRVMGWGCRDELWFARREAATLVTVACERPADEACFCAEVGLGPSHPRGSDL
                     LLVARPGGYAVEEITEKGRALVAAYPHRFREDERATLDELTAAEGPARIDLAAVQRWL
                     SDHFDDETWNEIALRCHGCGACASVCPACHCFDIVDEPDSLLAGTRRRNWDSCQASRF
                     TVHASGHNPREGQHARVRQRVMHKFQVFPKRFGRVLCTGCGRCTRACPAGQDLFETLT
                     LVSARALETP"
     gene            complement(1245..2114)
                     /locus_tag="PKK95_13085"
     CDS             complement(1245..2114)
                     /locus_tag="PKK95_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOC19202.1"
                     /translation="MRELRELAARLLEANDVQVVIGWEEWGRGVRPAFARTLAEADRL
                     VFDQRCVHNLATYLHPRRKHIRRLGRPAVVVKGCDARAAAGLIRDNQLRREDVVLIGV
                     RCPGMWVEGGAEGLPGDRKWADRCVSCPQRTPTLCDHLVGEDGPEPDGPGLEAQVERL
                     QALPMAERWAFWSSAFERCVRCYACREVCPLCFCDRCLADKSVPQWIESSPHQRGNVA
                     WHLARALHLAGRCVGCGECERACPAGIPLSILNARLQQSVRERFEFVASDDPSVPSPI
                     GQFRVDDGQEFIR"
     gene            complement(2102..2548)
                     /locus_tag="PKK95_13090"
     CDS             complement(2102..2548)
                     /locus_tag="PKK95_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOC19203.1"
                     /translation="MAAFQPKITAFVCNWCTYTGADLAGTSRLQMAPEVRIIRLPCTG
                     RIDPLFIIKAFERGADGVIVSGCHPADCHYNHGNYHARRRFAIFRDLLDFVGIDPRRV
                     IFSWVSASEGGKWRDVVDGATATIRELGPCRTDTGDEGQGRLACAS"
     gene            complement(2566..4590)
                     /locus_tag="PKK95_13095"
     CDS             complement(2566..4590)
                     /locus_tag="PKK95_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOC19204.1"
                     /translation="MARIGVFVCHCGENIGRTVDASAVAEALRSHPGVAHAEDYRYMC
                     SDPGQTLVREAIAANRLTGVVVAACSPHMHERTFRKVASAAGLNPFLCEMANIREQCS
                     WIHDDREQATRKATAITRTLVEKVKANAPLDTIRIPIERRALVIGGGIAGIQAALDLA
                     DGGAEVVLVEKESSIGGHMSQLSETFPTLDCSQCILTPRMVEVAQHPRIRLYAYSEVE
                     EVSGYIGNFEVKVRRKARSVDEALCNGCGACTQACPNRKVPSEFECGLGKRPAISVPF
                     PQAVPNLPVLDRARCARFMALRKGIAKAVCGKCAEACTRGAIDFGQEDRFVTEKVGAI
                     VVATGYQLYSIGKEQPEGEGLSGYGEYGYGAVPDVIDGLQLERLASASGPTGGRIVRP
                     SDGRVPKRVVFLQCVGSRDPARGIPYCSKICCMYVAKHVMLYRHKVHDGEAVVFYMDV
                     RAAGKGYDEFTRRAIEEEGAIYRRGRVARLWRDGEVVKVQGFDTLAGEPLLLDADLVV
                     LATAVRPQPGIRALAQKLSVSCDQHGFINEAHPKLRPVETNTAGIFVAGACQAPRDIP
                     ESVAMGSAAAAKVLGLFSNAELEREPTVARVDAAHCAGCFLCERVCPYGAVGRREVRD
                     RAGRVLKVVAEVNPGVCQGCGTCQATCPGKHVELQGFTDEQIYAEIAALG"
     gene            complement(4590..>4968)
                     /locus_tag="PKK95_13100"
     CDS             complement(4590..>4968)
                     /locus_tag="PKK95_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOC19205.1"
                     /translation="AMEQIARACGAAPVAWHMGLECCGGSLSVSRTGSVIRLGRAILE
                     DARRSGAEALLVACPMCHSNLDFRQRAMARDGRPPIPVLYVTELVGLALGIEAGRLGL
                     DRHFVSAAPLLAARALAPAGGRH"
BASE COUNT          720 a         1828 c         1729 g          691 t
ORIGIN      
        1 ggcgatgcag aaggtgcact cgccggcgcc gaaaaccgag tactcgccga actggccggc
       61 cttgaacgag aagccggcgg ccaggtcggc gtcgcggaag gcgagccgca gcgtccgcgt
      121 gtcgggcgtc tcgtcctgga tcgcctcgat ggtcatcagg tggggttgat acaggctcat
      181 cgcgttgtgt ccgggcggcc acaagggccg cccctacggg gtctcgagcg cgcgcgccga
      241 gacgagcgtg agagtctcga agaggtcctg gcccgcgggg cacgcgcggg tgcaccggcc
      301 acagccggtg cacaggacgc ggccgaatcg cttcgggaac acctggaact tgtgcatcac
      361 gcgctggcgg acgcgcgcgt gctgcccttc gcgcgggttg tgccccgagg cgtgcaccgt
      421 gaagcgcgaa gcctggcagc tgtcccagtt cctgcggcgc gtgcccgcga gcagcgagtc
      481 gggttcgtcc acgatgtcga agcagtggca cgcggggcac acgctcgcgc aggcgccgca
      541 gccgtgacag cggagcgcga tctcgttcca ggtttcgtcg tcgaagtggt cggacagcca
      601 gcgctgcacc gccgccagat cgatccgggc cgggccctct gccgctgtca attcgtcgag
      661 cgtcgcccgc tcgtcctcgc ggaagcggtg cgggtacgcc gccacgagag cgcgcccctt
      721 ctcggtgatc tcctccacgg cgtagccgcc ggggcgcgcg accagcagca ggtcggaacc
      781 gcgcgggtgc gacggcccca gcccgacctc ggcgcagaag cacgcctcgt ctgcaggacg
      841 ctcgcaggcc accgtcacca gcgtggcggc ctcgcggcgc gcgaaccaca actcgtcgcg
      901 gcagccccag cccatcaccc ggtccatcgc gtccgcaccg gccgcatcgc acggccgcgc
      961 gccgatcacg acgactcttc cgctcggcgg cgccggcgga acgagccgga cctccgccgc
     1021 cgtgcgctcc caccagaaca gcggctcggt ggggggaagg aagacccgct tcaagggcag
     1081 gagcgggagg ccggcgttca ggtcgagctc ggcggcctcg cgaagcggac ggtactccac
     1141 gccaggccgc gacgttcccg gcactcgcgc ggggccccat accgtggagc cggccgccag
     1201 caggtcggcc gccagtccct gcaggtcggc ctcggagatg actgtcatcg gatgaactcc
     1261 tgaccgtcgt ccacgcggaa ctggccgatc ggtgagggca cggaggggtc gtccgaggcg
     1321 acgaactcga agcgctcgcg gaccgactgc tgcagccgcg cgttgaggat cgacaacggg
     1381 atcccggccg ggcaggcgcg ctcgcactcg ccgcagccga cgcagcggcc cgccaggtgc
     1441 agcgcacgcg cgaggtgcca ggcgacgttg ccccgctggt ggggagagct ttcaatccac
     1501 tggggcaccg acttgtcggc caggcaccgg tcgcagaagc acagggggca gacctcgcga
     1561 caggcgtagc acctgacgca tcgctcgaag gccgacgacc agaaggccca gcgctcggcc
     1621 atcggcagcg cctggagccg ttccacctgc gcctcgaggc cgggtccgtc gggctcgggg
     1681 ccatcctcgc cgaccaggtg gtcgcagagc gtgggcgtcc gctgtgggca gctgacgcag
     1741 cggtcggccc attttcggtc cccgggcagg ccttcagccc cgccctccac ccacatcccc
     1801 ggacacctca ccccgatcaa caccacgtcc tcgcggcgca gctggttgtc gcggatcagc
     1861 cccgcggccg cgcgcgcgtc gcaccccttc acgacgacgg cgggccgtcc cagccggcgg
     1921 atgtgcttgc gccgcgggtg caggtaggtc gccaggttgt gcacgcagcg ctggtcgaaa
     1981 accaggcgat ccgcttcggc gagcgttcgg gcgaacgccg gccgcacgcc gcgcccccac
     2041 tcctcccagc cgatgaccac ctgcacgtcg ttggcctcga gcagccgggc tgccagctct
     2101 ctcagctcgc gcacgccagc ctcccttgcc cctcgtctcc ggtgtcagtg cggcagggtc
     2161 ccagctcgcg gatggtggcg gtggcgccgt ccaccacgtc gcgccacttg cctccttcgg
     2221 aagccgacac ccaggagaag atcacgcggc gcgggtcgat gccgacgaag tcgagcaggt
     2281 cgcggaagat cgcgaagcgc cgccgcgcgt ggtagttgcc gtggttgtag tggcagtcgg
     2341 ccgggtggca gccgctgacg atgacgccgt cggcgccgcg ctcgaacgcc ttgatgatga
     2401 acagcggatc gatgcggccg gtgcagggca gccggatgat gcggacctcg ggggccatct
     2461 gcagccggct ggtgcccgcg aggtcggcgc cggtgtaggt gcaccagttg cacacgaacg
     2521 ccgtgatctt cggctgaaac gcagccatgg aacctcccag taccctcatc ccaacgccgc
     2581 gatctccgcg tagatctgct cgtctgtgaa cccttgcagc tcgacgtgct ttccggggca
     2641 ggtggcctgg cacgtgccgc acccctggca gacgcccggg ttgacctcgg ccacgacctt
     2701 cagcacccgg ccggcacggt cgcggacctc gcggcggccg acggcgccgt acgggcagac
     2761 ccgctcgcac aggaagcagc ccgcgcagtg ggctgcgtcc acgcgggcta ccgtcggctc
     2821 gcgctcgagc tcggcgttcg agaagaggcc gagcaccttg gccgccgccg cgctgcccat
     2881 cgcgaccgac tcggggatgt cgcgcggcgc ctggcaggcg ccggccacga agatccccgc
     2941 ggtgttcgtc tcgacggggc gcagcttcgg gtgcgcctcg ttgatgaagc cgtgctggtc
     3001 gcacgagacc gagagcttct gggcgagcgc ccggattccc ggctgcgggc gcaccgccgt
     3061 cgccagcacc accaggtcgg cgtcgagcag cagcggctcg ccggcaagcg tgtcgaaccc
     3121 ctggaccttg acgacctcgc catcgcgcca caggcgtgcg acgcggccgc gccggtagat
     3181 cgcgccctcc tcttcgatcg cccgccgcgt gaactcgtcg taccccttgc ccgcggcgcg
     3241 cacgtccatg tagaagacca ccgcctcgcc gtcgtgcacc ttgtggcggt acagcatcac
     3301 gtgcttggcg acgtacatgc agcagatctt cgagcagtag gggatgcccc gtgccgggtc
     3361 gcgcgacccc acgcactgca ggaataccac gcgcttcggc acccggccgt ccgacgggcg
     3421 cacgatccgg ccgcccgtcg gccccgacgc cgaggcgagg cgctcgagct gcaggccgtc
     3481 gatcacgtcg ggcacggcgc cgtacccgta ctcgccgtat cccgagagac cctcgccctc
     3541 cggctgctcc ttcccgatcg agtagagctg gtacccggtg gcgacgacga tcgcccctac
     3601 cttctcggtc acgaaccggt cttcctggcc gaagtcgatc gcgccacgcg tgcaggcctc
     3661 ggcgcacttc ccgcagaccg ccttcgcgat ccccttgcgc aatgccatga accgtgcgca
     3721 ccgcgcgcgg tcgagcaccg gcaggttcgg gaccgcctgc gggaacggca ccgagatcgc
     3781 gggccgtttc cccaggccgc actcgaattc gctcggcacc ttgcggttcg ggcacgcctg
     3841 cgtgcaggcg ccgcacccgt tgcacagcgc ctcgtcgacc gagcgggcct tgcggcgaac
     3901 cttcacctcg aagttgccga tgtagcctga cacctcctcg acctcgctgt aggcgtagag
     3961 gcggattcgc gggtgctggg cgacctccac catccggggc gtcaggatgc actgcgagca
     4021 gtcgagcgtg gggaaggtct cggagagctg gctcatgtgc ccgccgatag acgactcctt
     4081 ctcgaccagc acgacctcgg ccccgccgtc cgccaggtcg agcgccgcct ggatgccggc
     4141 gattccgccg ccgatcacga gcgcccgccg ctcgatgggg atccggatgg tgtcgagcgg
     4201 ggcgttcgct ttcaccttct cgaccagcgt gcgcgtgatc gcggtcgcct tgcgcgtggc
     4261 ctgctcgcgg tcgtcgtgga tccacgagca ctgctcgcgg atgttggcca tctcgcagag
     4321 gaaggggttg aggccggccg ccgacgccac cttgcggaac gtccgctcgt gcatatgcgg
     4381 cgagcaggcg gcgacgacaa cgcccgtcag gcggttggcg gcgatcgcct cgcggaccag
     4441 cgtctggccc gggtccgagc acatgtagcg gtagtcctcg gcgtgggcca cgcccgggtg
     4501 gctgcgcagg gcttcggcaa ccgccgaggc atccacggtg cggccgatgt tctcgccgca
     4561 gtgacagacg aacacaccga tgcgcgccat cagtgcctcc cgcccgccgg cgcgagtgcc
     4621 cgcgccgcga gcagcggcgc cgcgctgacg aagtgacggt cgagccccag tcgccctgcc
     4681 tcgatcccca gcgcgagccc gaccagctcg gtgacgtaga ggacgggaat cggcgggcgg
     4741 ccgtcccgcg ccatcgcacg ctggcggaag tcgaggttcg agtggcacat cgggcaggcc
     4801 acgagcagcg cctcggcgcc cgacctgcgc gcgtcctcga ggatggcccg cccgagccgg
     4861 atgaccgatc cggtgcggct gaccgagagc gatccgccgc agcactcgag gcccatgtgc
     4921 cacgcgacgg gggccgcgcc gcacgcccgg gcgatctgct ccatcgcg
//
LOCUS       DAOPQX010000275         4948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18565_length_4948_cov_4.859800, whole genome shotgun sequence.
ACCESSION   DAOPQX010000275 DAOPQX010000000
VERSION     DAOPQX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18565_length_4948_cov_4.859800"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..924)
                     /locus_tag="PKK95_13105"
     CDS             complement(94..924)
                     /locus_tag="PKK95_13105"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOC19206.1"
                     /translation="MHRKLAAVAAVLLLAASCAGRQPSAPTTAPAARAARGGPVTFRA
                     MSYNIRHGEGLDGRLDLARIASVITGENADLVALQEVDRGVERTARRDLPAELAALTG
                     MTAIFSNNHPHQGGEYGNAVLTRFPVRRWANTHLRMLRPAEQRGVLQVVVDVHGRDLL
                     FLCTHIDYRRDDAERLLNVEQFGEVLAQYEGLPALFGGDFNDTPGSRTYLAMSKTFDE
                     VWAQVGEGDGFTIPSAAPAKRIDYLWVRKGSTLRPLRAWVPETEASDHRPLVAEMKLE
                     "
     gene            1011..1928
                     /locus_tag="PKK95_13110"
     CDS             1011..1928
                     /locus_tag="PKK95_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC19207.1"
                     /translation="MPGQASFGIEPPGYRLPAVARVGAVHLQVQQLARSLAFYQDVLG
                     LRVVERDQVSAALGAPGTGGAGGADAAEPPLLSLRARPGTRPARPGSLGLYHFAILLP
                     SREALGGLIAHLETLGVPFGGGDHLVSEAVYLSDPDGLGIEVYADRPREQWRVRGREL
                     VMATEPLDADDLVRAAGGRGWEGLPAGSRVGHVHLSVGDLEQAEAFYHRALGLDKVVW
                     SYPGALFFSAGGYHHHLGANTWGRGEAAGAEEARLLSWELQLPGGDAVEEAARSLEAA
                     GYSVERSGTGWSASDPWGQALHCVPGPIS"
     gene            2308..3540
                     /locus_tag="PKK95_13115"
     CDS             2308..3540
                     /locus_tag="PKK95_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19208.1"
                     /translation="MTVAQLIYPGDRYADRAARDLALAVLQQTVFGSGPGGEGWTADA
                     FLERLGAADGGASDLLDSVVSIEELQHVPLPPALTDGHGRLSTPARVEYLNDLAVAIR
                     DVAVTNLAGLKPEHTRALEGLVAARLGPPLGLNGARHLLSALAGRLRGVRLGVGLDKQ
                     EFERQRADLAPRYAAAEIEASVTGNGAGKYRQLVDEEAVLVFDIERHDAAIDLFTHLM
                     DRVAEWRSQVIALAGACDGLIEELLDEAEVARRRRPDLGPTAYEIDAPMRGAPRPDPE
                     IEGARFLAWLAAERQGGPAALLPLGRGELKDLLLRWAAGHEAVRAVRDRRIDEVLSSI
                     SADDRRRHLRQLVALAAAAGSAENGGPPAVYVFGVEDVERTSLSQAEVNQLVPPAAAA
                     RMVSTADSRRIFCIGVAG"
     gene            complement(3728..4102)
                     /locus_tag="PKK95_13120"
     CDS             complement(3728..4102)
                     /locus_tag="PKK95_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOC19209.1"
                     /translation="MARWSDRYSEVTYFLLRVVAGLLFAQHGVQKVFGLLGGKAVPLH
                     SIYGLAGVLELVGGVLIALGIQAGWVALILAGEMAVAYFWRHAPRALFPIQNGGELAA
                     LYSFIFLYISARGGGRWRLSGR"
     gene            4241..>4948
                     /locus_tag="PKK95_13125"
     CDS             4241..>4948
                     /locus_tag="PKK95_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19210.1"
                     /translation="MNGWKEIAAHFGRTVRTVQRWERELGLPVHRLSAGKGETVFALP
                     AELDGWLVQNERAPGGPGSADPNGSGSREIDPGSGPGALPARPAGRSFASLLAVFLVG
                     VAVIAIVLLVAWHPPGTVGGERRVPSSFKRDGQQLTVLDQSGQVLWTWRAPFPLSERE
                     YSDQPGVPAFPRVLFTDLEGDGSIETVFCALGGVLADSAVYVFEQDGTMRFGWEYQGQ
                     KRFGDVTYSSPWRIAGVV"
BASE COUNT          677 a         1753 c         1792 g          726 t
ORIGIN      
        1 agcaccttct gcacctccgg caccagcacc ttctgcacct ccggcaccag cacctccagc
       61 accttctgca ccccctgcac ctcctgcacc tctctattcc agtttcatct ccgccaccaa
      121 cggccggtgg tcggacgcct cggtctcggg cacccaggca cggaggggcc ggagggtcga
      181 accctttcga acccagaggt agtcgatccg tttggccggc gcggcgctgg ggatggtgaa
      241 gccgtccccc tcgccaacct gggcccacac ctcgtcgaac gtcttcgaca tcgccaggta
      301 ggtccggctg ccgggcgtgt cgttgaagtc gccgccgaag agcgcgggca gcccctcgta
      361 ctgcgccagc acctcgccga actgctccac gttcagcagc cgctcggcat cgtcgcgccg
      421 gtagtcgatg tgcgtgcaga ggaagaggag gtcgcggccg tgtacgtcca ccaccacctg
      481 cagcacgccg cgctgctcgg ccgggcgcag catccgcagg tgcgtgttgg cccagcgccg
      541 caccgggaag cgggtgagga cggcgttgcc gtactcgccg ccctggtgcg ggtggttgtt
      601 gctgaagatg gcggtcatcc cggtcagcgc cgccagctcg gcgggcaggt cgcggcgggc
      661 cgtgcgttcg acgccgcggt ccacctcctg cagggccacc aggtcggcgt tctcgcccgt
      721 gatgaccgag gcgatgcggg ccagatcgag ccggccgtcg agtccctcgc cgtggcggat
      781 gttgtagctc atggcgcgga aggtgacggg gccgccgcga gcggcacgtg cggcaggggc
      841 ggtggtgggc gcagagggct ggcgccccgc gcaggaggcg gcaaggagaa ggacggcagc
      901 gacggcggca agcttccggt gcatcgccgg atcgtacagc cgcgcttcgg gatcgcaaag
      961 aaaagtggcg gcggttgccc tctgcctgcc tcccggtcaa gatgaaggtt atgcccggcc
     1021 aagcctcctt cggcatcgag ccccctggat accggctgcc ggcggtggcg cgcgttggcg
     1081 cggtccacct gcaggtgcag cagctcgccc ggtcgctcgc cttctaccag gacgttctcg
     1141 gcctgcgggt cgtcgagcgc gaccaggtgt cggctgcgct cggcgcgccc ggcaccggcg
     1201 gtgccggcgg cgcggacgcc gcggagcccc cgctgctctc gctgagagcg aggccgggaa
     1261 cgcggccggc aagaccgggg tcgctcgggc tctaccactt cgccatcctg cttccctcgc
     1321 gcgaggcgct gggcggcctc atcgcgcacc tcgagacgct gggcgtgccg tttggcgggg
     1381 gagatcacct ggtgagcgag gcggtctacc tttcggatcc cgacggcctc ggcatcgagg
     1441 tgtacgcgga ccggccgcgc gagcagtggc gggttcgagg ccgcgagctg gtgatggcca
     1501 ccgagccgct ggacgccgac gacctggtgc gggctgccgg cggccgcggc tgggaggggc
     1561 tgccggccgg ctcgcgcgtc ggccacgtcc acctgagcgt gggcgatctc gagcaggccg
     1621 aggcgttcta ccaccgggcc ctcgggctcg acaaggtggt gtggagctac ccgggcgcgc
     1681 tgttcttctc ggccggcggc taccatcacc acctgggcgc caacacctgg gggcgcggcg
     1741 aggcggccgg cgccgaggag gcgcggctgc tgtcgtggga gctgcagctg ccgggcgggg
     1801 acgcggtcga ggaggctgcc cgcagcctcg aggcagccgg gtattccgtc gagcgctccg
     1861 gtacgggctg gagtgcgtcc gacccctggg gccaggccct gcactgcgtc ccggggccca
     1921 tctcttgagc gctctgctcc tggccgccgc cgctgtcggg cggcgcggtt gccgccggca
     1981 gtagcgccgg cctccgtgcc ggccggcctc cgggcggtcg cctcggtcac cttctccgcg
     2041 ccctccggtc agcatttcga aagaattgaa gcttgctagt cgcgccacga cgcgacaaat
     2101 cacgacatga gcccgatgtc gctccttgac gcttgcctcc aggccggcgc gggacgagac
     2161 tgctgccgtc cccatccccc atttctcgta agtcccctcg gcctgcgggc cggccgagtg
     2221 accccggctg tcgcatccac ccgggacact gctctggcag gtgctgaaag agagcctggc
     2281 aacactgtcc cttctgcgag ccgttcgatg actgtggcgc aactcatcta tcccggggat
     2341 cgctacgccg accgggccgc cagggacctg gccctggccg tcctgcagca gaccgtcttc
     2401 gggagcggtc ccgggggcga gggctggacg gccgacgcgt ttctcgagcg gctcggcgcg
     2461 gctgacggcg gggcctccga cctgctcgac tcggtggtct cgatcgagga gctgcagcac
     2521 gtcccgcttc ccccggcgtt gaccgacggc cacggccggc tgtccacgcc ggcccgggtc
     2581 gagtatctca acgacctcgc ggtggcgatt cgcgacgtgg ccgtcacgaa cctcgcgggg
     2641 ctgaagcccg aacacacgcg ggcgctcgag gggctggtcg ccgctcgtct cggccccccg
     2701 ctcgggctca acggcgcccg gcacctgctg tcggcgctcg ccggccggct gcgcggcgtg
     2761 cgccttggcg tgggcctcga caagcaggag ttcgagcggc agcgcgccga tctcgcgccc
     2821 cgctacgcgg ccgccgagat cgaggcgagc gtgacgggca acggcgcggg caagtaccgc
     2881 cagctggtgg acgaggaggc ggtgctcgtc ttcgacatcg agcggcacga cgcggccatc
     2941 gacctgttca cgcacctgat ggaccgggtg gccgagtggc gcagccaggt gatcgcgctg
     3001 gccggagcgt gcgacggcct catcgaggaa ctgctcgacg aggcggaagt ggcccgccgg
     3061 cggcgccccg acctgggtcc gaccgcgtac gagatcgacg cgccgatgcg cggggcgccc
     3121 cggcccgatc ccgagatcga gggcgcgcgc ttcctcgcct ggctcgcggc cgagcgccag
     3181 ggcggaccgg ccgcgctgct gccgctcggg cgcggcgagc tgaaggacct gctgctgcgg
     3241 tgggcggccg gccacgaggc ggtcagggcc gtgagggaca ggcgaatcga cgaggtgctg
     3301 tcgtcgattt cggccgacga ccgccgccgc cacctccggc agctggtggc gctcgcggcc
     3361 gccgccgggt cggcggaaaa cggcggacct cccgcggtct acgtcttcgg cgtcgaggac
     3421 gtggagcgca cgtcgctctc gcaggccgag gtgaaccagc tcgtgccgcc agccgcggcg
     3481 gcgcgcatgg tgtctacggc tgattcgcgg cggatcttct gcatcggcgt ggccggctag
     3541 gaacttggaa cgcgaaggtc gggctagccc gccgaagccg cagcctgccc gccgtacccg
     3601 cgaacgaagt gagcgtttgg cgaaggcggg agcgtagcga gcgtttggcg aaggtcgggt
     3661 cgccacccgg ggggcagatc gtgaacaggc cacctttcac gatctgaccc tcgcgtggtt
     3721 tcgggcgtca gcggccgctc aggcgccagc ggccgccgcc gcgcgcggag atgtagagga
     3781 agatgaaaga gtagagcgcg gccagctcgc cgccgttctg gatcgggaag agcgcgcgcg
     3841 gcgcgtgacg ccagaagtag gccaccgcca tctcgccggc cagaatgaga gccacccagc
     3901 ccgcctggat gccgagcgcg atcaacacgc cgcccaccag ctcgagcacg cccgccagcc
     3961 cgtagatcga gtggagcggc accgccttcc ccccgagcag cccgaacacc ttctgcacgc
     4021 cgtgctgcgc gaacaacaaa cccgccacca cgcggagcag gaaatacgtg acttccgagt
     4081 accggtcgga ccaacgggcc atggggcgac tcctcgttga agtttcagga agatccatcc
     4141 taccccagca ggcggccggc cggcccgctg gaaccaaggg ggcccgcgat gctatgttct
     4201 gtcgttcatg gacccggcgg ttcggcccca cacccttcgc ttgaacggct ggaaggagat
     4261 tgcggcccac ttcggccgta cggttcgcac cgtccagagg tgggagcgcg agctgggcct
     4321 gccggtccac cgcctctccg ccggcaaggg ggagacggtc ttcgcgctgc cggccgagct
     4381 ggacgggtgg ctcgtccaga acgagcgcgc ccccggcggt cctggatcgg ccgacccgaa
     4441 cggaagcgga agccgtgaga tcgacccggg cagcgggccc ggcgcccttc ccgcccgacc
     4501 cgccggccgc agctttgcca gcctcctcgc tgtgttcctg gtcggcgtcg cggtgatcgc
     4561 gatcgttctc ctcgtggcgt ggcacccgcc gggcacggtg ggcggcgagc gacgggtccc
     4621 ctcgtcattc aagcgggacg gccagcagct gaccgtgctc gatcagtcag gccaggtcct
     4681 ctggacgtgg cgcgcgccct tcccgctgag cgaacgcgag tactccgacc agccgggggt
     4741 gcccgcgttc ccgcgggtgc tgttcaccga tctcgagggc gacggctcga tcgaaacggt
     4801 gttctgcgcc ctcggcggcg tgctcgccga ctcggccgtg tacgtcttcg agcaagacgg
     4861 cacaatgcgc ttcggctggg agtaccaggg gcagaaacgg ttcggcgacg tcacctattc
     4921 ttcgccatgg aggatcgccg gggtcgtc
//
LOCUS       DAOPQX010000276         4925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18664_length_4925_cov_4.510062, whole genome shotgun sequence.
ACCESSION   DAOPQX010000276 DAOPQX010000000
VERSION     DAOPQX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18664_length_4925_cov_4.510062"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PKK95_13130"
     CDS             <1..1121
                     /locus_tag="PKK95_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19211.1"
                     /translation="TFLELAAWYGSVGRTADARRVLALAPGATEVLYWRAWLAALEND
                     AEAATLLREAEAASPEMVFPFRAESLDVFEWAAQKAPGWKAKYYLALVRWALNDLDGA
                     RELLERVGDEPRYAPFYAARAEAMAAVSRARARADLERAIDLDPRAWRFGKRLAEHLL
                     QDGEVEQARQVAARYAAAEPSNYVIGMLHARTLLRAGRYAEADALLRRLEVLPFEGAI
                     EGRLLYREAQLMLGVESLRASRFDSLLERVKAAREWPEHLGAGKPYPEQVDERLEDWL
                     QALGLERSGRTAEAEAVWKRVSADRRPGFGLLLNALALKRLGRAAEAGRALDEWASAA
                     KDSSLAAWGKSVFAGSPVPLPPAVRDLEPRVFAHVSSY"
     gene            1229..4279
                     /locus_tag="PKK95_13135"
     CDS             1229..4279
                     /locus_tag="PKK95_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19212.1"
                     /translation="MAMRGCRRAPGLVLLLLAAPGAAAAQHRTGTVEVRVEDTAGRPA
                     AGTFVTAAPEAGGESRHCTTRHDGRCVMLAVAPGTYRVFAGAASAAGDAAATVAPGEH
                     TVLRLAPRAAEGHTLRAAGVPGGPPPASTVLDRQGLLDLPTRRTLFDALRFDLTQPSP
                     GNWWLDAGGTFAPLRIFIDGVEYPASHSRGLHPGADALDALRLDTPERPGASSARGAG
                     GAISAAWRAGASRLSGEGSLVYEGWRLNGPPRPFSRYSPWDRNLAERNLTVPATPWAD
                     LTPRFNLGGPLGPPRLSFHASGSFSRRDHHREVVFLEDPDRAARRFSWFSWSAQGSAN
                     LTAARSRGGRVRLAMSFGRSRNRGSAPTLEPDQGRLPDGSPTAGLTRAPFAGPAETQA
                     RWRDTGADARRFTLSPSIDWPIGEAWSLAAGAALSFPSSWTPRAFRGTGTRRVFATSN
                     AKLPGISPDEVQAAGFADNPSSYGTVRDTTRRAVLSLEANWAPGRRRSAHLFSAGVRA
                     ERTSDEVYIGHEHPVIRLHWNRALTTTSGSSARGTYGYYTVTRPGTIGRAAGTSAAAW
                     LQDRWSPGRRLSIEAGLRAEREAAPSYVEGSGVRTPVFGFGDMLAPRLSIAWKPDDAG
                     AWRVQGWFGLYFDHLTTRLARTLFGARHDVIESWTLDTTNWRTLECDEGRRDCPGEFI
                     ERVDRRVPWNVADPLLARSSGLTTRIDPGLRPMQTGEWGAGIERRLARSTSLALGYTG
                     KWLVRAVEDVGLSLPGVGERAVLANPGFGCATTVAPGHPAFPLPKATRRYHELEIELR
                     RRGRPVALAARYRWSRLAGNYGGLAASDEGGRQSTNLTSAFDALYSSYDRFGRQVDGP
                     LPGDRPHRLGLDATLALPSGTTLSVSALLESGRPESSEVTFRGVPVWFNGGGDLGRQP
                     PFSQVDARVRQDVRVAAWVVTLEASVDNVFDQSASLGFFSNNPYRDALAVPEEAFFEF
                     PWDPAAWATMLRTEGVRVRDEQLFLEPNRFQRPRRVVLTAALRF"
     gene            4481..>4925
                     /locus_tag="PKK95_13140"
     CDS             4481..>4925
                     /locus_tag="PKK95_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19213.1"
                     /translation="MRRAVTCISAARIALDAGHLLEAEERLEEACLLDPEGLEARILL
                     EQLRSREPDEAEEPALALPEVDAQGSGAVRRWSHWVAVLIAVVALAALAWWRAPSPDE
                     SRIVPAQRPAEPRSEPGPDLPRTVDLQPGGGTEVWPEGHVDLLGIG"
BASE COUNT          625 a         1793 c         1784 g          723 t
ORIGIN      
        1 agacattcct cgagctggcc gcctggtacg ggtcggtcgg gcgcacggca gacgcgcgcc
       61 gcgtcctggc gctcgcgcct ggggcaaccg aggtgctgta ctggcgcgcg tggctcgcgg
      121 cgctcgagaa cgacgccgag gccgcgacgc tgctgcgcga agcagaggcc gcctctcccg
      181 agatggtatt cccgttccgg gcggagagcc tcgacgtgtt cgagtgggcc gcgcagaagg
      241 cgccgggctg gaaggcgaag tactacctcg cgctcgtgcg gtgggcgctc aacgacctgg
      301 acggcgcgcg cgagctgctc gaacgagtgg gcgacgagcc gcgctacgcg ccattctacg
      361 cggcgcgtgc ggaggcgatg gccgcggtgt cgagagcacg cgcccgggcc gatctcgagc
      421 gcgcgatcga tctcgacccg cgcgcgtggc gcttcgggaa gcggctggcc gaacacctgc
      481 tccaggacgg cgaggttgaa caggcccgcc aggtggcggc gcggtacgcc gcggccgagc
      541 cgtcgaacta cgtcatcggc atgctccacg cccgcacgct gctgcgggcc gggcggtacg
      601 ccgaggccga cgccctgctg cggcggctcg aggtgctgcc cttcgagggg gcgatcgagg
      661 gacgcctgct gtaccgcgaa gcgcagctca tgctcggggt cgagtcgctg cgcgcgtcgc
      721 ggttcgacag cctcctcgag cgcgtgaagg cagcccgcga gtggcccgag cacctcggtg
      781 ccggcaagcc gtacccggag caggtggacg agcgcctgga ggactggttg caggcgctcg
      841 gactcgaacg gtcggggcgg accgccgagg ccgaagcggt ctggaagcga gtgagcgctg
      901 accggcgtcc gggcttcggc ctgctgctca acgcgctggc gttgaagcgg ctcggccgcg
      961 cggccgaggc cggccgcgcg ctcgacgagt gggcgtcggc cgccaaggac tcctcgctgg
     1021 ccgcgtgggg gaaaagcgtc ttcgccggct cgcccgttcc tctgcccccg gcggtccgcg
     1081 atctcgagcc gcgggtgttc gcccacgtct cgtcgtactg aggagtgttt tcctgattcc
     1141 tgattcctga ttcctgattg ctgttgcgtg caatcgcggg cacccgccgc gagggtgagg
     1201 gcgcggcgtc ctacactggc ggggcgaggt ggccatgcga ggttgccgcc gtgcgcccgg
     1261 tctcgtcctg ctgctgctcg ccgcgccggg ggccgccgcg gcgcagcacc gcaccggaac
     1321 ggtggaggtg cgagtcgagg acaccgccgg caggccggcc gccggcacct tcgtcaccgc
     1381 agcccccgag gccgggggtg agtcacgcca ctgcacgacc cgccacgacg ggcggtgcgt
     1441 gatgctggcc gtcgccccgg gcacgtaccg cgtcttcgcg ggggccgcga gcgcggcggg
     1501 cgatgccgcg gccaccgttg cgccgggcga gcacacggtg ctgcggctcg caccccgggc
     1561 tgcagaaggg cacacgctga gggcggcggg cgttcctgga gggccgcctc ccgcgagtac
     1621 ggtgctcgac cgccagggcc tcttggacct gccaacgcgc cgaacgctgt tcgacgccct
     1681 gcgcttcgac ctcacgcagc cctcgcccgg caactggtgg ctggatgccg gcggcacgtt
     1741 cgccccgctg cgtatcttca tcgacggggt ggagtacccg gcgtctcaca gtcgcggcct
     1801 ccatcccggc gccgatgcgc tggacgctct tcgtctcgac acacccgaac gccccggggc
     1861 gagctcggcg cgcggcgccg gcggcgcgat ctccgcggcc tggcgcgcgg gagcgtcgcg
     1921 cctctcaggc gaggggtcgc tcgtctacga ggggtggcgc ctgaacggtc cgccgcgccc
     1981 gttctcccgc tactcgccgt gggaccgcaa cctcgccgag cgaaacctca ccgtgccggc
     2041 cacgccctgg gccgatctca cgcccaggtt caatcttggc ggaccgctcg ggcctccgcg
     2101 cctctccttt cacgcctccg gctcgttcag ccggcgcgac caccaccgcg aggtcgtgtt
     2161 cctggaggat cccgaccggg ccgcccgccg cttctcgtgg ttctcctggt ccgcgcaggg
     2221 gagcgccaac ctgaccgccg cccgctcgcg cggcggccgg gtccggcttg cgatgtcgtt
     2281 cgggcgcagc aggaaccgcg gaagcgcccc gacgctcgag cccgaccagg gccgcctgcc
     2341 cgacggctcg ccgaccgcgg gcttgacgcg cgcgccgttt gccgggcccg cggaaaccca
     2401 ggcccgctgg cgcgacacgg gcgccgacgc gcggcgcttc acgctctcgc cttcgatcga
     2461 ctggccgatc ggggaggcgt ggtcgctcgc cgccggagcg gcgctgtctt tcccgagctc
     2521 gtggacgccc cgcgcgtttc gcgggaccgg aacccggcgc gtcttcgcca cctcgaatgc
     2581 caagctcccc ggcatctcgc cggacgaggt gcaggcagcc ggctttgccg acaacccctc
     2641 cagctacggc accgtgaggg acacgacccg gcgcgccgtg ctgtcgctcg aggccaactg
     2701 ggcgcccggc cgccgccgat ccgcgcacct gttctccgcc ggcgtgcggg ccgagcgcac
     2761 ttccgacgag gtctacatcg gccacgagca cccggtgatc cgcctccact ggaaccgggc
     2821 gctcaccacc acgagcggga gcagcgcgag gggcacgtac ggctattaca cggtgacgcg
     2881 gcctgggacg atcggacgcg ccgcgggcac cagcgcggcc gcctggctgc aggaccggtg
     2941 gtcgcccggc cgccgcctga gcatcgaggc tggcctgcgc gccgagcgcg aggcggcgcc
     3001 ttcctacgtc gaaggctcag gcgtccgcac gcccgtcttc ggcttcggcg acatgctggc
     3061 gccccgactg tcgattgcgt ggaagccgga tgatgccggc gcatggcgcg tgcaagggtg
     3121 gttcgggctc tacttcgatc acctcacgac acggctggca cggacgctct tcggcgcccg
     3181 ccacgacgtg atcgagtcgt ggacgctcga caccacgaac tggcgaaccc tcgagtgcga
     3241 cgaggggcgg cgcgactgcc ccggggagtt catcgagcgc gtggatcgcc gggtgccgtg
     3301 gaacgtcgcc gacccgctgc tggcccgctc gtccggcctg acaacccgca tcgacccggg
     3361 gctgcggccg atgcagactg gggagtgggg ggcgggcatc gagcgccggc tcgcccgctc
     3421 cacgtcgctg gcgctgggct acacggggaa gtggctcgtc cgggcggtgg aggacgtggg
     3481 gctgtcgctt ccgggcgtgg gcgagcgtgc cgtgctcgcg aaccccggct tcgggtgcgc
     3541 gacgacggtc gcgcccggcc atcccgcatt cccgctgccg aaagccacgc gacggtatca
     3601 cgagctcgaa atcgagctgc gccgacgcgg ccggcccgtc gctctcgccg cgcgttaccg
     3661 ctggagccgg ctggcgggca attacggggg gcttgcggcg tcagacgagg gggggcgcca
     3721 gagcaccaac ctgaccagcg cgttcgacgc cctctactcg agctacgaca ggttcggccg
     3781 gcaggtcgac ggccccctgc ccggcgaccg tccccaccgg ctgggtctcg acgccacgct
     3841 cgccctgccc tccggcacca cgctctcggt ctcggcgctg ctcgagtcgg gccgccccga
     3901 gtcgtctgaa gtcaccttcc gcggtgtgcc cgtgtggttc aacggaggcg gcgacctggg
     3961 ccgccagccg cccttctcgc aagtcgacgc cagggtgcgt caggacgtcc gggtggccgc
     4021 ctgggttgta acgctcgagg ccagcgtcga caacgtcttc gaccagtccg cctctcttgg
     4081 cttcttcagc aacaacccct accgcgacgc tctggccgtg ccagaagagg cctttttcga
     4141 gttcccgtgg gatcctgccg cctgggccac catgctccgg accgaaggcg tccgagtcag
     4201 ggacgagcag ctcttcctcg agccgaaccg cttccagcgc ccgcggcgcg tggtcctgac
     4261 ggcggccctg cgcttttgac cgtggcactc ggtaaacgcc agaggggggg acgccgtatg
     4321 aggtgggtag acccgaagct acccacctcc tttggggtcc ctgcccggca gggaccccgt
     4381 ttttcccctc ctttcccgga tcccctcttg cacacggctc ccgcgtattc ccatgaggcc
     4441 accggacgac tcacgttccc agttcgagca gcggatgcgt ttgcggcggg cggtgacctg
     4501 catctcggcc gctcgaatcg ccctcgacgc tggccacctg ctcgaggcgg aggaacgact
     4561 ggaggaggcc tgtctcctcg accccgaggg cctcgaggcg cgcatcctgc tcgagcagct
     4621 gcgctcgcgc gagcctgacg aggcggagga accagcactg gcgctgcctg aggtagacgc
     4681 ccagggatcg ggagcggtga ggcgttggag ccattgggtt gccgtcctga ttgccgttgt
     4741 ggcgcttgcc gccctggcct ggtggcgcgc accgagcccc gacgagagcc gaatcgttcc
     4801 ggcccagcgt ccggcggagc ccaggagcga acctgggccc gatctgcctc ggaccgtcga
     4861 tctgcagccg ggcggcggta ctgaggtttg gcccgagggc catgtggacc tgctggggat
     4921 tgggc
//
LOCUS       DAOPQX010000277         4924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18670_length_4924_cov_5.176628, whole genome shotgun sequence.
ACCESSION   DAOPQX010000277 DAOPQX010000000
VERSION     DAOPQX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18670_length_4924_cov_5.176628"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKK95_13145"
     CDS             complement(<1..644)
                     /locus_tag="PKK95_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19214.1"
                     /translation="MIEPLPAFVGVNLPWHTYGCDFGGNAWFPEGGVGRPEVAGQLDE
                     LFTRLAAAGLRVVRWFLFCDGRAGIRFDPVGVPLGLDSYCFRDVDAALALAASHRATL
                     LFVLFDFTWFRPRRVVNGVELFGRRRVQVRETSRRALLERVVVPLLARYGREPAILGW
                     DLCNEPEWAAFGYGSVAPWRTIAAGTMSDWLREMASAAHAEAVQPVTVGLASRRG"
     gene            complement(654..1205)
                     /locus_tag="PKK95_13150"
     CDS             complement(654..1205)
                     /locus_tag="PKK95_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19215.1"
                     /translation="MTAYPFQPEAPARKDVPASPGRLWKRIPLERRMEAAAAFWEDEE
                     GAEQQAEALLAIASHFHFRLKTTRTLPLERKVRYLASLPGLSDPVAGRVLVAYHLRHR
                     RPMLAAFLDALGITHDNGVLSGEELQAPEASRLAEAATKLRAEYPKEDVELYFQTLLV
                     QDPDTWGGLAELLQDEQKPGPEA"
     gene            complement(1279..2649)
                     /gene="glmM"
                     /locus_tag="PKK95_13155"
     CDS             complement(1279..2649)
                     /gene="glmM"
                     /locus_tag="PKK95_13155"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOC19216.1"
                     /translation="MPAIPTLKISISGVRGVVGDSLTPDLLTRFSQAFGTYIGSGRVV
                     VGRDTRTSGEMVRQAVVAGLLSTGCRISDAGVCPTPTVQLLVRHLRAHGGIAITASHN
                     PPEWNALKFVGPDALFLSGGRGRELLDIYHQGDYTKARSHRLRGVEDVPDAVDLHLDA
                     VLGAVGPLPAGRRPKVVIDSCNGAGAVAGPRLLERLGAEVIGINVTPDGSFPRPAEPT
                     AENLRSLCDAVRRHGADIGFAQDMDADRLAVVSEEGTPIGEERTLLLAIEHVLSQTPG
                     PVVVNLSTTRAVEEVAARYGCRACRTAVGEANVTAGMQRFRAVIGGEGNGGVIYPAIN
                     FARDSLVGMALILHRLVSSGQRVSELAAALPPFEMVKMQFPFPSQRLRDVLRRAAREY
                     QAYPQDLRDGVKVTLPDGWFLLRGSNTEPVMRVIAEADDAGAAHALAARVREQVEAWV
                     TEPPRP"
     gene            complement(2690..4039)
                     /gene="glmM"
                     /locus_tag="PKK95_13160"
     CDS             complement(2690..4039)
                     /gene="glmM"
                     /locus_tag="PKK95_13160"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOC19217.1"
                     /translation="MKPLKIGIGGVRGVVGETFTPELVVGFAQAFGSYLGRGRILVCR
                     DTRPSGPMVAAAVTAGLLATGNEVVDLGICPTPSLQLAVGWLGAVGGVSVTAGHNPAH
                     WNALKFVRHDGIYLTASQAEELLDVYHQGRFDKAGWDAIPRRVEKAEVIGHHLEVLRA
                     QFDVARVRARRFRVAVDCCNGPCSLLSPRWLEELGCEVLAINDDPAGPFAHTPEPKPE
                     NAVQVRAVVKAGRADLGLVHDGDGERVSLVDEGGRALSEEMTLPLAADMALQERVGPV
                     VTNVCTTMAVDLVAARYGAPVVRTPVGQPFISEAMLDHRAVIGGEGNGSVAVPRVQPT
                     HDSAATIGLLLSHLASAERPLSALADALPQTVMVKEHVPIEPNLIYRALQDFRDAVQE
                     EAEGRTDLADGVKVGFPDGWVHVRASNTESMLRLIVEAHGTARARELVDWAHDRLGR"
     gene            complement(4036..>4924)
                     /locus_tag="PKK95_13165"
     CDS             complement(4036..>4924)
                     /locus_tag="PKK95_13165"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19218.1"
                     /translation="MVAVYLVLSDLNGLERLEAYRREIRSPEDLAGYLGTIENGQSFG
                     ALAGDRGVGTFGGSEDHTAILCGREGELSLYSFCPVHHERQVSFPADHGLVVAVSGIV
                     AEKTGAAMASYNRASFAARAVLEAWNRATGRSDPCLGRAVAKAGPSPIRRAIASNTPE
                     GFDAGALLDRFEQFYQESEEILPAAAQALDECRLGDFGRLVDRSQALAERLLGNQVPE
                     TIALAHSARDLGAVAASAFGAGFGGSVWALVPSAAASAFTESWQRAYRAACPGPAGAA
                     RFLFTRPGPGAFRLPVETP"
BASE COUNT          709 a         1851 c         1707 g          657 t
ORIGIN      
        1 ccgcgccgcg aagcgaggcc gacggtcacg ggctgcaccg cctcggcatg cgcggccgac
       61 gccatctcgc gcagccagtc cgacatggtg cctgccgcga tcgtccgcca cggcgcgacg
      121 ctgccgtacc cgaaggccgc ccactcgggc tcgttgcaca ggtcccagcc gaggatcgcg
      181 ggctcgcggc cgtagcgggc caggagcggc acgacgaccc gctccagcag cgcgcggcgg
      241 ctggtctccc gcacctgcac tctccggcgc ccgaacaact cgacgccgtt caccacgcgc
      301 cgaggccgga accaggtgaa atcgaagagg acgaagagaa gcgtcgcacg gtgcgaggcg
      361 gccagcgcca gcgccgcgtc gacgtcgcgg aagcagtagg agtcgaggcc gagtggaacc
      421 ccgaccgggt cgaacctgat gccggcccgc ccgtcgcaga agagaaacca tcggacgacg
      481 cgcaggccgg ccgccgcgag gcgggtgaac agctcgtcga gctgccccgc cacctcggga
      541 cggccgacac cgccctcggg gaaccaggcg ttgccgccga agtcgcaacc gtaggtgtgc
      601 caggggaggt tgacgccgac gaaggccggg aggggctcga tcacggccgg tcgtcaggcc
      661 tcggggcccg gcttctgctc gtcctggagc agctcggcca accctcccca ggtgtccggg
      721 tcctggacga ggagcgtctg gaagtacagc tccacgtcct ccttcgggta ctcggcgcgc
      781 agcttcgtcg cggcctcggc gagccgcgag gcctcgggcg cctggagttc ctcgccactg
      841 agcacgccgt tgtcgtgcgt gatgccgagc gcgtcgagga acgcggccag catcggccgc
      901 cggtgacgca ggtggtaggc caccaggaca cgccccgcga ccggatcgga gagcccgggc
      961 agcgaggcga ggtacctcac cttccgctcg agcggaagcg tccgggtggt cttcaggcgg
     1021 aagtggaagt gcgaggcgat ggcgagcagc gcctcggcct gctgctcggc cccctcctcg
     1081 tcttcccaga acgccgccgc cgcctccatg cggcgctcga gcgggatgcg cttccagagc
     1141 cggccgggac tggccggcac gtccttcctc gcgggggctt ctggctgaaa cggatacgcc
     1201 gtcatgatgt ctcccggtct tcagtcaacc cttgatggta gcacgcgccg cgcgcccact
     1261 ggggactgga ggcccgggtc aaggccgggg cggctcggtc acccaggcct cgacctgctc
     1321 gcgcacgcgg gcggcgagcg cgtgcgccgc cccggcgtca tccgcctcgg cgatcacgcg
     1381 catgaccggc tcggtattcg agccgcgaag caggaaccac ccgtcgggca gggtcacctt
     1441 cacgccgtcc ctgaggtcct gcgggtacgc ctggtactcg cgggcggccc gccgcagcac
     1501 gtcccgcagg cgctgcgacg ggaacggaaa ctgcatcttc accatctcga agggcggcag
     1561 cgccgcggcc agctccgaga cgcgctgccc cgacgacacc agccggtgca ggatgagggc
     1621 catgcccacc aggctgtcgc gcgcgaagtt gatcgccggg tagatcactc ccccatttcc
     1681 ttctcccccg atcaccgccc ggaatcgctg catcccggcg gtcacgttcg cttcgcccac
     1741 ggccgtccgg cacgcgcggc acccgtagcg ggccgctacc tcctcgacgg cccgagtggt
     1801 cgacaggttg acgaccaccg gtccgggcgt ctgcgagagc acgtgctcga tcgccagcag
     1861 gagcgtccgc tcctcgccaa tcggcgtgcc ctcctccgag accaccgcca ggcgatccgc
     1921 gtccatgtcc tgcgcgaacc cgatgtccgc gccgtgacgg cgaaccgcgt cgcacaagct
     1981 gcgcaggttc tcggcggtcg gctcggcggg ccgcgggaag ctgccgtccg gggtgacgtt
     2041 gatcccgatg acctcggcgc ccaggcgctc aagcagccgg ggcccggcca cggccccggc
     2101 gccgttgcag gaatcgatga cgaccttcgg gcggcgtccc gcgggcagcg gccccaccgc
     2161 cccgagcacc gcgtcgaggt gcaggtcgac ggcgtcgggc acgtcctcca cgccgcgaag
     2221 ccggtgcgaa cgcgccttcg tgtagtcgcc ctggtggtag atgtcgagca gttcgcgacc
     2281 gcgcccgccg ctgaggaaca gggcgtcggg accgacgaac ttcagcgcgt tccactcggg
     2341 cgggttgtgg cttgccgtga tggcgatgcc gccgtgcgcg cggaggtgcc gcaccagcag
     2401 ctgcaccgtc ggcgtcgggc aaacgccggc gtccgagatc cggcagccgg tcgacagcaa
     2461 gccggcgaca acggcctggc gcaccatctc gcccgaggtg cgggtgtctc gcccgaccac
     2521 cacacggccc gacccgatgt aggtgccgaa cgcctgggag aagcgcgtga ggagatccgg
     2581 ggtgagcgag tcgcccacga ccccgcggac gcccgagatg ctgatcttca gtgtcggtat
     2641 ggccggcatc gtccgtctcc ctggcggccc cccgggaagc gaggcctcgt taccgtccca
     2701 gccgatcgtg cgcccagtca accagctctc gggcgcgggc cgtgccgtgc gcctcgacga
     2761 tcaggcgcag catcgattcc gtgttcgagg cgcgcacgtg cacccagcca tccgggaagc
     2821 cgaccttcac cccgtccgcc aggtcggtcc gcccctcggc ctcctcctgc acggcgtcgc
     2881 ggaagtcctg cagcgcgcgg tagatcaggt tgggctcgat cggcacgtgc tccttcacca
     2941 tcaccgtctg gggcagggcg tccgccaggg ccgagagcgg ccgctcggcc gaggcgaggt
     3001 gcgagagcag cagcccgatg gtggcggcgc tgtcgtgggt cggctgcacg cgcggcaccg
     3061 cgacgctgcc gttgccctcg ccgccgatga cggcccggtg atccagcatc gcctcggaaa
     3121 tgaacggctg gccgaccggc gtgcggacga ccggggcgcc gtagcgcgcc gccaccaggt
     3181 cgacggccat cgtcgtgcac acgttggtca cgaccgggcc gacgcgttcc tgcagcgcca
     3241 tgtcggccgc cagcgggagc gtcatctcct cggagagcgc tcgtcccccc tcgtccacca
     3301 ggctcacccg ctcgccgtcg ccgtcgtgga cgaggccgag gtccgcccgc cctgccttga
     3361 cgaccgcccg cacctgcacg gcgttctcgg gcttcggctc gggtgtgtgc gcgaaggggc
     3421 ctgcgggatc gtcgttgatc gcgagcacct cgcaccccag ctcctcgagc cagcgcggcg
     3481 acagcagcga gcaggggccg ttgcagcagt ccaccgccac cctgaagcgc cgcgcccgca
     3541 cgcgcgcgac gtcgaactgc gcgcgcagca cttcgaggtg gtgcccgatc acctcggcct
     3601 tctcgaccct cctggggatc gcgtcccacc ccgccttgtc gaagcgcccc tggtgataga
     3661 cgtcgagcag ctcctcggcc tgggaggccg tcaggtagat cccgtcgtgg cgcacgaact
     3721 tgagcgcgtt ccagtgcgcg gggttgtggc cggccgtcac cgacacgccg cccacggccc
     3781 cgagccagcc gaccgccagc tgcaggctgg gtgtcgggca gatgccgaga tccaccacct
     3841 cgttgccggt cgccaggagg cccgccgtga ccgcggcagc caccatgggc cccgagggcc
     3901 gggtgtcgcg acacacgagg atgcggccgc gcccaaggta gcttccaaac gcctgcgcga
     3961 agccgacgac cagctcgggg gtgaacgtct cgcccacgac accccgcacg cctccaatcc
     4021 cgatcttcag cggtttcatg gtgtctccac cggcagccgg aacgcaccgg gtcccgggcg
     4081 cgtgaacagg aaccgggcgg cgccggccgg gccggggcac gcggcgcgat aggcgcgctg
     4141 ccagctttcg gtgaacgcgc tggccgcggc gcttgggacg agggcccaga cgcttccgcc
     4201 gaaacccgcc ccgaaggcgg aggcggccac cgcacccagg tcgcgggcgc tgtgcgcgag
     4261 cgcaatcgtc tcgggcacct ggttccccag caggcgttcc gccagcgcct gcgatcgatc
     4321 cacgaggcgg ccgaagtccc cgagccggca ctcatccagg gcctgcgccg cggcgggaag
     4381 gatctcctcg ctctcctggt agaactgctc gaagcggtcg agcagcgcgc cggcgtcgaa
     4441 gccctcgggc gtgttcgacg cgatcgcgcg acggatcggc gacggccccg ccttggcgac
     4501 tgcgcgcccg aggcagggat cgctcctccc ggtggcgcgg ttccacgcct cgagcaccgc
     4561 acgggccgcg aacgacgcac ggttgtacga ggccatcgcg gcccccgtct tctcggccac
     4621 gatcccgctc acggccacca cgagcccgtg gtcggccggg aagctcacct gccgctcgtg
     4681 gtgcacgggg cagaacgagt agaggctcag ctcgccctcg cgcccacaga ggatcgcggt
     4741 atggtcctcg ctccctccga aggtccccac gccgcggtcc cctgccagcg ccccgaagct
     4801 ctgcccgttt tcgatcgttc cgaggtaccc cgccaggtct tccggcgacc ggatctcgcg
     4861 ccggtaggcc tccaggcgct cgaggccgtt caggtcggac agcaccaggt agaccgccac
     4921 catg
//
LOCUS       DAOPQX010000278         4914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18725_length_4914_cov_5.169788, whole genome shotgun sequence.
ACCESSION   DAOPQX010000278 DAOPQX010000000
VERSION     DAOPQX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18725_length_4914_cov_5.169788"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /gene="amrB"
                     /locus_tag="PKK95_13170"
     CDS             complement(<1..1102)
                     /gene="amrB"
                     /locus_tag="PKK95_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOC19219.1"
                     /translation="MTKAGRGVFALVIAAIAGAAAAAVRGEAQQGPGPGLAAVREPAV
                     AGSFYPAEARALGAALDGYLADAVPAAGHRPLALVAPHAGYVFSGQIAADAWAQARGH
                     QYDAIVLLGTNHTGVRADRVSVFDGEGLKTPLGTVRNDASLVAALLANCRDCVADRSA
                     HVREHSIEVQLPFARRLFPDTPVVAAVVGPGNPALAERFGRTLAAALRGRKGLIVASS
                     DLSHYPPARQAAEVDRRTVEAILSMDPAQVTFALRAAERTGVPGLATCACGEAPVLAA
                     IAAAREMGAERAHAVSYANSADVAVGEPERVVGYAAVAFTAGPAGKDVSGLPQRRPSS
                     REPVSPEDRARLLVLARTSIDRYLASETLPLPR"
     gene            complement(1099..2292)
                     /gene="amrS"
                     /locus_tag="PKK95_13175"
     CDS             complement(1099..2292)
                     /gene="amrS"
                     /locus_tag="PKK95_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOC19220.1"
                     /translation="MLTRRQLLERAASLGAGAPALGLAPLAWPAAGGQGLVPPAPRAR
                     YWASGADAGKDCLRCHPAGEVLKAGHRHRAAAIKCLLCARGCTILPGGRGACRARANV
                     GGELKSLVYGRPLSITVDPIEKKPFYHFLPGSDAFSFGTSGCPLRCRFCQNWELSQSS
                     PGDFSPAYRTPADLARAASLRRSPIVAFTYNEPTVFTEYMLDVAAESRQRGIRSVMVS
                     CGLMNEAPLADLCAALSAIKIDLKGFSEPFYRDVCGAELRPVLRSIRQIAATKVHLEI
                     VNLVVPSLNDSDRALRDLVSFVAGELGPDVPLHFSRFYPDYQLLHLPPTPVSTLERAR
                     DLGMAAGLHYVYIGNIPGHAGNHTYCPACRKVVVRREGFFVTGQHLVAGRCEYCRAPV
                     KGVWA"
     gene            complement(2302..2718)
                     /locus_tag="PKK95_13180"
     CDS             complement(2302..2718)
                     /locus_tag="PKK95_13180"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOC19221.1"
                     /translation="MAVSIDIDYLGDLHCAATHGPSGSRLTTDAPTDNGGRGELFSPT
                     DLVATALGTCLTTVMGLVARRTGLDLEGTRVHVEKHMTSEPVRRIGALVAVITFPRDF
                     SPGDRVRLERAAQICPVRQSLHPEVRVELRFEYRSE"
     gene            2792..2968
                     /gene="tatA"
                     /locus_tag="PKK95_13185"
     CDS             2792..2968
                     /gene="tatA"
                     /locus_tag="PKK95_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOC19222.1"
                     /translation="MRVGLPELVIILVILLLLFGTSRLAGLGKGLGQAIRGFKNEVGP
                     RDNGDEEPEVRGRK"
     gene            complement(2990..4267)
                     /locus_tag="PKK95_13190"
     CDS             complement(2990..4267)
                     /locus_tag="PKK95_13190"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC19223.1"
                     /translation="MAHLTARGAYRSLVERLNRFPQGAPPSEVLYAILQMLFSEREAG
                     LVARLPIKPFTAAKAARAWQMPEAEAREVLDALAGRAILLDIERPDGVRHYVLPPPMA
                     GFFEFSMMRLRGDLDQALLARLFYQYLNVEEDFIKALFLTGETQLGRVFVNEGALPEE
                     LSLHVLEYERASEVVKEASCIGISLCYCRHKMQHAGKACDVPLDICMTFNTVASSLTR
                     HGAARRVEVSECLDLLQQARERGLVQFGENVRRRPSFICNCCGCCCEALLAARRFGFL
                     HPVHTTNYLPSVDKGRCNGCGACVEACPVEAMALVSANDPVRRRARTARVNEDLCLGC
                     GVCVPACKRGGLVLRERPARVITPVNSTHKVVLMAIERGKLQDLVFDNHALASHRAMA
                     AVLGVILKLPPVKRALASRQLRSRYLETLLARQ"
     gene            complement(4290..>4914)
                     /locus_tag="PKK95_13195"
     CDS             complement(4290..>4914)
                     /locus_tag="PKK95_13195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOC19224.1"
                     /translation="IKRHGGVVGVRTDKGGYATETVVNAAGAWARPIAQSAGLDTPVA
                     PDSHEAGITEPVARFFDPMLVDLRPTPGARNYYFYQHGPGGIVFCITPDPPIVGTDRR
                     ETSAYLPMIATRMVGLMPKLANVKVRRTWRGLYPMTPDGSPILGWSKQVEGYVHAEGM
                     CGQGFMLGPGVGATLSRMIRGVETETDRTILRELAPDREFGGDEALK"
BASE COUNT          723 a         1663 c         1830 g          698 t
ORIGIN      
        1 cgcgcgggag cggcagcgtc tcgctggcca ggtatcggtc gatgctggtg cgtgcgagga
       61 cgagcaggcg cgcgcggtcc tctggcgaca cgggttcgcg cgaggatggc cggcgctgcg
      121 gcaggcccga gacgtccttc ccggccggcc ccgcggtgaa cgccaccgcg gcgtatccca
      181 cgacgcgttc gggttctcca acggcgacgt cggccgagtt ggcgtagctc acggcgtggg
      241 cccgctccgc gcccatctcg cgcgcggcgg cgatcgcggc caggacaggc gcctcgccgc
      301 aggcgcacgt cgcgagaccg ggaacgccgg tgcgctcggc ggcccggagc gcgaaggtca
      361 cttgcgcggg atccattgag aggatcgcct cgaccgttcg gcggtcgacc tccgccgcct
      421 gccgtgcggg agggtagtgg gagagatccg agctggccac gatcaaaccc ttgcgcccgc
      481 gcagcgcggc ggcgagggtc cggccgaacc gctcggcgag cgccgggttg cccgggccga
      541 ccacggccgc gacgacgggc gtatcgggga agaggcgccg ggcgaacggc agctggacct
      601 cgatcgagtg ctcgcgaaca tgggcggagc ggtcggcgac gcagtcgcgg caattggcga
      661 ggagcgcagc gacgagcgag gcgtcattcc gcacggttcc cagcggcgtc ttcagccctt
      721 caccgtcgaa gacggagacc cggtctgctc gcacgccggt gtggttggtg ccgagcagca
      781 cgatggcgtc gtactggtga ccgcgggcct gcgcccacgc gtcggcggca atctgtccgg
      841 agaagacgta gccggcgtgg ggagcgacga gcgcgagcgg ccggtggccg gccgcgggaa
      901 cggcgtcggc aaggtagccg tcgagcgccg cgcccagggc gcgtgcctcg gccgggtaga
      961 agctgccggc gacggccggc tcgcgcacgg ccgccagccc cggccccggg ccctgctgag
     1021 cctcgccgcg cacggccgcg gcggcggctc ccgcgatcgc ggcgatgact agcgcgaaga
     1081 cgcctcgacc ggccttcgtc atgcccatac ccctttgacc ggtgcccggc agtactcgca
     1141 tcgtccggcc accaggtgct gcccggtcac gaagaacccc tcgcgccgaa cgacgacctt
     1201 ccggcatgcc gggcagtagg tgtgattgcc ggcgtggcct gggatgttgc cgatgtacac
     1261 gtaatggagc cccgcggcca tcccgagatc cctggcccgc tccagcgtgc tgaccggggt
     1321 cggtggcagg tgcaggagct ggtagtcggg atagaagcgg gagaagtgga gtggcacgtc
     1381 ggggcccagc tcgccggcga cgaacgacac gaggtcgcgg agggcgcgat ccgagtcgtt
     1441 cagcgacggc accacgaggt tgacgatctc gaggtgaacc ttggtggcgg caatctgccg
     1501 gatgctgcgg aggacgggcc gaagctcagc cccgcacacg tcgcggtaga acggctcgct
     1561 gaaccccttg aggtcgatct tgatcgcgga cagtgccgcg cacaagtccg cgagcggcgc
     1621 ctcgttcatc agcccgcagc tcaccatcac ggagcggatc ccgcgctggc gcgactccgc
     1681 cgcgacgtcg agcatgtact cggtgaagac cgtcggctcg ttgtaggtga acgcgacgat
     1741 cgggcttcgg cgaagcgagg ccgcgcgagc caggtcggcg ggcgttcggt aggcggggga
     1801 gaagtcgccg ggagacgact gcgacagctc ccagttctgg cagaaccggc atcgcagcgg
     1861 gcagcccgag gtgccgaagg agaaggcgtc gctgccgggc aggaagtgat agaagggctt
     1921 cttctcgatc ggatcgaccg tgatcgagag cgggcgcccg tagacgaggc tcttcaactc
     1981 gccgcccacg ttggcccgcg cgcggcaggc gccgcgcccg cccggaagga tcgtgcatcc
     2041 ccgggcgcaa aggaggcact tgatcgccgc agcgcggtga cggtgcccag ccttcaggac
     2101 ctcgccagcc gggtggcagc ggaggcagtc cttgccggcg tcggcccccg acgcccagta
     2161 gcgcgcccgc ggggcgggcg gcaccagccc ctgcccgccg gccgcgggcc aggcaagcgg
     2221 ggccaggccc agggcgggcg cgccggcccc caggctcgcg gcacgctcga gcagctggcg
     2281 gcgggtgagc atgggcggat gttactccga tcggtactcg aacctcagct ccactcgcac
     2341 ctccgggtgc aggctctgcc ggaccgggca gatctgcgcg gcgcgctcga gccgcacccg
     2401 gtcgccgggg ctgaagtcgc gtggaaaggt gatcaccgcg acgagggccc cgatgcggcg
     2461 gacgggctcg gaggtcatgt gcttctcgac gtgcacgcgc gtgccctcga ggtcgaggcc
     2521 ggtccggcgc gcgacgagcc ccatgacggt cgtcaggcag gtgccgagcg cggttgccac
     2581 gagatcggtg ggcgagaaca gctcgcctcg accgccgttg tcggtggggg catccgtggt
     2641 gaggcggctt cctgaggggc cgtgtgtcgc cgcacagtgg aggtcgccga ggtagtcgat
     2701 gtcgatgctc accgccatgg cgggattcta tcggattacg ctcccgtcgc cgcctgtgtt
     2761 atcgttgctg cgacggtttc aaggagtgtc gatgcgagtg ggtctgccag aactggtcat
     2821 catcctcgtc atcctcctgc tcctcttcgg caccagccgg ctggctgggc tggggaaggg
     2881 gcttgggcag gcgattcgcg gcttcaagaa cgaggtgggg ccgcgcgaca acggcgacga
     2941 agagcccgag gtcaggggcc ggaagtagcc tccctcattc cctcccgccc tactggcgcg
     3001 cgagcagtgt ctccaggtag cgcgatcgga gctggcggct ggccagcgcc cgcttgaccg
     3061 gcggcagctt caggatcacg ccgaggacgg ccgccatggc gcggtggctg gccagggcat
     3121 ggttgtcgaa gaccaggtcc tggagtttcc ctcgctcgat cgccatcagc accaccttgt
     3181 gcgtcgagtt gaccggggtg atgacgcgcg ccggccgctc gcgcagcacc agtccgccgc
     3241 gcttgcaggc gggaacgcac acgccgcagc cgaggcagag atcctcgttc acccgggccg
     3301 tcctggcccg ccgccgaacc gggtcgttgg ccgacaccag cgccatcgcc tcgactggac
     3361 acgcctcgac gcacgccccg cagccgttgc agcggccctt gtccaccgac ggcaggtagt
     3421 tcgtggtgtg gacggggtgc aggaagccga agcggcgcgc ggcgagcagc gcctcgcagc
     3481 agcagccgca gcagttgcag atgaagctgg ggcggcggcg cacgttctcg ccgaactgca
     3541 cgaggcccct ttcgcgggcc tgctggagca gatcgaggca ctcggacacc tcgacgcggc
     3601 gcgccgcgcc gtgccgcgtc agcgacgagg cgaccgtgtt gaacgtcatg cagatgtcga
     3661 gcgggacgtc gcacgccttt ccggcgtgct gcatcttgtg gcggcagtag cacaggctga
     3721 tgccgatgca gctcgcctcc tttaccacct cactcgcgcg ctcgtactcc agcacgtgga
     3781 gcgacagctc ctcgggcagc gccccctcgt tcacgaacac gcgcccaagc tgcgtctcgc
     3841 ccgtcaggaa cagcgccttg atgaagtctt cctcgacgtt caggtactgg tagaagaggc
     3901 gggcgagcag cgcctggtcg agatcgcccc ggagccgcat catcgagaac tcgaagaagc
     3961 cggccatcgg cggcggaagc acgtagtggc gcacgccgtc cggccgctcg atgtcgagca
     4021 ggatcgcccg gccggcgagc gcgtcgagca cctcgcgggc ctcggcctcg ggcatctgcc
     4081 aggcgcgggc tgccttggcg gcggtgaacg gcttgatcgg gaggcgtgcg acgaggccgg
     4141 cctcgcgctc gctgaagagc atctggagga tcgcgtagag gacctcggag ggcggcgccc
     4201 cctgcgggaa gcggttcagc cgctcgacga ggctccggta cgcgccacgc gcggtgaggt
     4261 gggccatggg ggactcacgg actacatttt catttcaagg cttcatcccc tccgaactcc
     4321 cggtcgggcg ccagctcgcg caggatcgtg cggtcggtct cggtctcgac gccgcggatc
     4381 atgcggctga gcgtggcccc cacgccgggg ccgagcatga agccctggcc gcacatcccc
     4441 tcggcatgga cgtacccctc gacctgcttc gaccagccga ggatcggcga cccgtcgggc
     4501 gtcatcgggt agagcccgcg ccaggtgcgc cgcaccttga cgttcgccag cttcggcatc
     4561 aggcccacca tccgggtggc gatcatcggc aggtaggccg acgtctcgcg gcggtcggtg
     4621 ccgacgatcg gcgggtcggg ggtgatgcag aagacgatgc cgcccgggcc gtgctggtag
     4681 aagtagtagt tgcgcgcgcc cggggtgggg cggaggtcca ccagcattgg gtcgaagaaa
     4741 cgggcgaccg gctcggtgat gccggcctcg tggctgtctg gcgcgacggg ggtgtcgagg
     4801 ccggccgact gggcgatggg gcgggcccag gcgcccgccg cgttgaccac cgtctcggtg
     4861 gcgtatcctc ccttgtcggt ccgcacgccg acgaccccgc cgtggcgctt gatg
//
LOCUS       DAOPQX010000279         4914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18728_length_4914_cov_3.705495, whole genome shotgun sequence.
ACCESSION   DAOPQX010000279 DAOPQX010000000
VERSION     DAOPQX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18728_length_4914_cov_3.705495"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1957
                     /gene="aspA"
                     /locus_tag="PKK95_13200"
     CDS             95..1957
                     /gene="aspA"
                     /locus_tag="PKK95_13200"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOC19225.1"
                     /translation="MDHPDIREFLARIELFGGLTAEELARIAAMVVVEDVKRGELLFE
                     EGAPRRHLHMIEKGEVELFRRGAGGESRLAFFEALDFLGEGSLLDDYPHSASARALQD
                     GRILRISRESFAGLLRDEPAVATKILSRTARVISRRLRRASARVADAGAQYISGRTRR
                     EHDLLGDREVPHEAYYGIQTLRAVENFEISGVPLHHYPVLIEALAMVKSACARANADL
                     GHLERPVEAAIVQACHEIIEGKWHGHFVVDMIQGGAGTSTNMNANEVIANRALEILGR
                     EKGDYAHCHPNNHVNLSQSTNDAYPSALRVALIRANGGLCRVLRDLADAFAAKALEFS
                     TVIKMGRTQLQDAVPMTLGQEFEAYAVTLGEEIERLEQNAALFLEVNLGGTAIGTGIN
                     ADPEFGSRAAARLSEIAGLEMRLAPHLIESTQDTGAFVMYSSAIKRLAVKLSKICNDL
                     RLLSSGPRAGLGEIQLPPMQPGSSIMPGKVNPVIPEVVNQIAYKVIGNDLTVTLAAEA
                     GQLELNVMEPVIVQSLLESIEMLKNGMDTLRLRCVTGIVANVERCREHVQRSIGVVTA
                     LNPILGYDTSTELAQEALATGRGVYDLVLQKGLLSREELDRLLSPEAMVRPRKL"
     gene            complement(2220..2522)
                     /locus_tag="PKK95_13205"
                     /pseudo
     CDS             complement(2220..2522)
                     /locus_tag="PKK95_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2597..3787)
                     /gene="corA"
                     /locus_tag="PKK95_13210"
     CDS             complement(2597..3787)
                     /gene="corA"
                     /locus_tag="PKK95_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOC19226.1"
                     /translation="MDRGEVKTGSTELESNAAPGAGNGRRRSRRRPALLRLLRMRRAK
                     KPGSVAGIELEELATLPSTTAPVGVTIFDYAPDQVQVTRVDDVDAFIGEHRPPWATVR
                     WINVDSVTNLHVIRALAEKYNLHPLAIEDVLHTTHRPKVEPYAEDGRYQPRLFIIVRM
                     LELVDGHLRSEQISIFLGHNTVLTFQESPGDVWDGVRQRLHARSRLRNGDASFLVHSL
                     IDAIVDSCFPMLEYYGDRLEEVEGEVLERARPESIKDIHDLKRELLLLRRAVWPIRDV
                     VSNLMREQHDCLSEQTRTYMRDIYDHTVQIIDIVETYREVAMGLTETYMTAMSNRLNE
                     VMKVLTMIGTIFIPLTFLAGVYGMNFDFLPELHWTWAYPAFWVISLATAAGMVAWFRR
                     KRWL"
     gene            3960..>4914
                     /locus_tag="PKK95_13215"
     CDS             3960..>4914
                     /locus_tag="PKK95_13215"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOC19227.1"
                     /translation="MTFRELVAGALVMGPMTKGSNLPYRRLCVELGARVTMSEMAVAR
                     RLRQKRRPEFALIRRAPGEPCFGVQLAGNKPEELGWAASLVEERGADFVDLNLGCPID
                     RFTRIGLGASLGRQPGRVRRLVEAMTRAVSRIPVTVKIRLGWNDEHRNYLEVACAAVD
                     GGAAGIAVHGRTRDARYRSKADWEAVAEVASAVGVPVIGNGDLLFGHDAPAAIARSGC
                     AAVMTARGALIRPWIFREAAEGYRPVGPDERLAVYRRYVELALEHWGDHERGRNQVRE
                     FLQWHLGFWCREAPRRADGSWPAMQERESRDAGRLSPLEALL"
BASE COUNT          746 a         1705 c         1729 g          734 t
ORIGIN      
        1 tggcccgtga aggaccggcg caccttcgag cactggcaga agcacaccgc ctgggggaag
       61 ctgttcgagc ggtattgacg gaaaggccgt ccccgtggat caccccgata ttcgcgagtt
      121 tctggcccgg atcgagctgt tcggcggcct gacggccgag gagctggccc gcatcgccgc
      181 catggtcgtc gtggaggacg tgaagcgcgg ggagctgctg ttcgaggagg gcgctccgcg
      241 caggcacctc cacatgatcg agaagggcga ggtcgagctg ttcaggcggg gggcgggggg
      301 tgagagccgc ctcgcgttct tcgaggcgct cgacttcctc ggcgagggca gcctgctcga
      361 cgactatccg cactcggcgt ccgcccgggc gctgcaggac ggccggatcc tgaggatcag
      421 ccgcgagtcg ttcgccgggt tgctgcgcga cgagcccgcg gtcgccacca agatcctctc
      481 gcgcaccgcc cgcgtgatct cgcggcgcct gcggcgggcg tccgcgcgcg ttgccgacgc
      541 cggcgcgcaa tacatcagcg gccgcacgcg ccgggagcac gacctgctgg gcgaccgcga
      601 ggtgccccac gaggcgtatt acggcatcca gaccctgcgc gccgtggaga acttcgagat
      661 cagcggcgtg ccgctccatc actacccggt cctgatcgag gccctggcga tggtcaagtc
      721 ggcctgcgcg agggccaacg ccgacctcgg ccacctcgag cgccccgtcg aggccgccat
      781 cgtgcaggcg tgccacgaga tcatcgaggg caagtggcac gggcatttcg tggtggacat
      841 gatccagggt ggcgccggca cctcgacgaa catgaacgcg aacgaggtga tcgccaaccg
      901 tgccctggaa atcctcggcc gcgagaaggg cgactacgcg cactgccacc ccaacaacca
      961 cgtgaacctc tcgcagtcca ccaacgatgc ctacccgtcg gcgctgcgcg tggcgctgat
     1021 acgggccaac ggcggcctct gccgcgtgct gcgcgacctg gccgacgcgt tcgccgcgaa
     1081 ggccctcgag ttctccaccg tcatcaagat gggccgcacg cagctgcagg acgccgtgcc
     1141 gatgacgctc ggccaggagt tcgaggcgta cgccgtgacg ctcggcgagg agatcgagcg
     1201 gctcgagcag aatgcggcgc tcttcctcga ggtcaacctt ggcggcaccg cgatcggcac
     1261 ggggatcaac gccgaccccg agttcggcag ccgcgccgcg gcacgcctct cggaaatcgc
     1321 cggcctggag atgcgcctgg cgccgcacct gatcgagtcc acccaggaca ccggcgcgtt
     1381 cgtcatgtac agctccgcca tcaagcggct cgcggtcaag ctctcgaaga tctgcaacga
     1441 cctgcgcctg ctctcgagcg gcccgcgagc cggcctcggc gagatccagc tcccgcccat
     1501 gcagcccggg tcgtcgatca tgccgggcaa ggtgaacccg gtgatccccg aggtcgtcaa
     1561 ccagatcgcc tacaaggtca tcggcaacga cctcaccgtg accctggcgg ccgaggccgg
     1621 gcagctcgag ctgaacgtga tggagcccgt gatcgtccag agcctgctcg agtcgatcga
     1681 gatgctcaag aacgggatgg acaccctgcg gctgcgctgc gtcacgggca tcgtggcgaa
     1741 cgtcgaacgc tgccgcgagc acgtccagcg aagcatcggc gtggtgacgg cgctcaaccc
     1801 gatcctcggg tacgacacga gcaccgagct cgcgcaagag gcgctcgcga ccggccgggg
     1861 agtgtacgac ctggtgctcc agaaagggct gttgtcgcgc gaggaactcg accggctgct
     1921 ctcccccgaa gcgatggtgc ggccgcggaa gctgtagccg ccgtgcacta ctcgtcccac
     1981 cggaaccggg ccaccgccgc cgccccgagg atggcagccg tcccgagcag cagcgcatag
     2041 gcccggctgg gccgccgcgc cgccgccggc agcggccagg tcgttccagc gcgtcacccc
     2101 gcccagcagc tcgcgcagcg cgcccttctc ggcctcggag agcccctcgg cctcgtccag
     2161 cccctcgagg gcgtcgctcg cggcggcccg cccggtcgga ccgctgaaca cctcccggct
     2221 caccgcttcc atcgcgtgcc cggccaggat gccctggacc atgcccgcct cggtcgccct
     2281 gctggcggcg aagaacagga ggttctcgcc gacagctcct cgacgatcgc cggctcctgc
     2341 ggaacgaggc cgatccgccg cttcagggga tcggtgtcgc cctgcaggcg gcgcccctcg
     2401 atcagcacct cgccctcgtc cggctccagc agccccgcca ccatcgagac ggtcgtcgtc
     2461 tttccggcac cgttcgggcc cagcagcccc accatctcgc ccggcccgac gcaaagcgag
     2521 acgccgttca cggcggcgag ggcgccgtac cgcttgacca gcccgcgcgc ttcgagcaca
     2581 ggcccctccg ttctcgctac agccagcgct ttcttctgaa ccacgccacc atcccggccg
     2641 cggtcgcgag gctgatcacc cagaacgccg ggtaggccca cgtccagtgc agctcgggca
     2701 ggaagtcgaa gttcatgccg tagacgcccg ccaggaacgt cagcgggatg aagatcgtgc
     2761 cgatcatggt cagcaccttc atgacctcgt tgagccggtt gctcatcgcg gtcatgtagg
     2821 tctcggtcag ccccatcgcg acctcgcggt aggtctcgac gatgtcgatg atctgcaccg
     2881 tgtggtcgta gatgtccctc atgtaggtgc gcgtctgctc gctgaggcag tcgtgctgct
     2941 cgcgcatcag gttgctcacc acgtcgcgga tcggccacac agctcgccgc agcagcagca
     3001 gctcgcgctt caagtcgtgg atgtccttga tcgactcggg ccgtgcgcgc tcgagcacct
     3061 cgccctcgac ctcctcgagc cgatcgccgt agtactcgag catcgggaag cacgagtcga
     3121 cgatggcgtc gatcagggaa tggaccagga agctcgcgtc cccgttgcgg agcctcgagc
     3181 gggcgtgcag ccgctggcgc accccgtccc acacgtcgcc cgggctctcc tggaaggtga
     3241 gcacggtgtt gtgcccgagg aagatgctga tctgctcgct tcgaaggtgg ccgtctacca
     3301 gctccagcat ccgcacgatg atgaagagcc gcggctggta gcgcccgtcc tccgcgtagg
     3361 gctcgacctt gggccggtgg gtggtgtgga ggacgtcctc gattgccagg ggatggaggt
     3421 tgtacttctc ggccagggcc cggatcacgt ggaggttcgt gacgctgtcc acgttgatcc
     3481 agcgcacggt cgcccacggc ggccggtgct cgccgatgaa ggcgtcgacg tcatcgacgc
     3541 gggtcacctg cacctggtcg ggcgcgtagt cgaagatggt cacgccgacc ggcgccgtgg
     3601 tcgaggggag ggtggccagc tcttccagct cgatgccggc caccgacccc ggcttcttcg
     3661 cgcgccgcat gcgcagcagc cgcaggagcg cgggccggcg ccgggagcgc ctccgcccgt
     3721 tgcccgcccc gggggccgcg ttcgactcca actcggttga cccggtcttc acttccccgc
     3781 gatccatgga acctccgaga ccccgcccga tcaccccggc gttccgagtc gggaggcctc
     3841 gagcgcgggc gaaaccggag atcatcgcac aagagtatag tgcgatgatg cgacgtggcg
     3901 gcgaaggcgg gagccggttg ccggcatccg gccggcccgc gatcggcgga ggacagggga
     3961 tgacgttccg cgagctggtg gccggggccc tggtgatggg cccgatgacg aagggctcga
     4021 acctgccgta ccggcgcctg tgcgtcgagc tgggcgcccg cgtgacgatg tccgagatgg
     4081 cggtggcgag gcggttgagg cagaaacggc gtccggagtt cgcgctgatt cgccgggcgc
     4141 cgggcgagcc gtgcttcggc gtgcagctgg ccggcaacaa gcccgaggag ctgggttggg
     4201 ccgcctcgct ggtggaggag cggggtgccg acttcgtgga cctcaacctg gggtgcccga
     4261 tcgatcggtt cacccgaatt ggcctcggcg cgtcgctcgg acggcagccg ggccgcgtgc
     4321 gccggctcgt cgaggcgatg acgcgggcgg tctcgcggat tcccgtgacg gtcaagatcc
     4381 ggcttgggtg gaacgacgag caccggaact acctcgaagt ggcgtgcgcc gcggtggacg
     4441 gcggcgcggc cgggatcgcc gtgcacggcc ggacgcgcga cgcccggtac cgttcgaagg
     4501 ccgactggga agccgtcgcg gaggtggctt ctgcagtggg ggtgccggtg attggaaacg
     4561 gggaccttct cttcgggcac gacgcgccgg cggcgatcgc gcggtcgggc tgcgcggcgg
     4621 tgatgacggc acgcggcgcg ctgatccgtc cctggatctt cagggaggcg gccgagggct
     4681 atcggcctgt gggccccgac gaacgcctgg ccgtctaccg gcgctatgtc gagctggccc
     4741 tcgagcactg gggcgatcac gagcggggaa gaaaccaggt gcgcgagttc ctgcagtggc
     4801 acctcgggtt ctggtgccgc gaggcgccgc ggcgagcgga cggctcgtgg cccgccatgc
     4861 aggaacggga atcgagggat gcgggtcggc tgtcgccgct cgaggcgctg ctcg
//
LOCUS       DAOPQX010000280         4913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18732_length_4913_cov_5.037052, whole genome shotgun sequence.
ACCESSION   DAOPQX010000280 DAOPQX010000000
VERSION     DAOPQX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18732_length_4913_cov_5.037052"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKK95_13220"
     CDS             <1..511
                     /locus_tag="PKK95_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC19228.1"
                     /translation="GREHLVRNLIYPVPDPRFPFLGVHFTRMVHGGVEAGPNAVLAFR
                     REGYTRTSFSPRDTLTLFEFPGFWRMGLKYWRMGLGEMYRSFSKRAFVAALRELVPAL
                     RDEDVRPSGAGVRAQALAPSGALLDDFHIVEAERMIHVLNAPSPAATASISIGRSIAG
                     MASRAFALK"
     gene            557..1897
                     /locus_tag="PKK95_13225"
     CDS             557..1897
                     /locus_tag="PKK95_13225"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOC19229.1"
                     /translation="MPETATLQVRGRTYTLPVVVGSEGEVAVDISKLRAESGVITLDD
                     GYANTGSCQSAITFIDGERGILRYRGIPIEQIAEHSTFPETAWLLIYGELPTADQLEC
                     WSNLLTRHEMLHEGLYKHFDGFPSGGHPMAILSAMINATSTYNPDIIRMESPETFQSA
                     AARLLSKTRTIAAAAYKTSIGQPLVYPKPQFDYASNFLHMMFSIPFKEYEPDPDVVAA
                     LSLILVLHADHEQNCSTSTVRMVASSGANLFASCAAGVCALWGPLHGGANTAVLEMLQ
                     YIVESGMSVKDYVERVKDRTSNIKLMGFGHRVYKNYDPRAKIIKQAADRVLGKLKIHD
                     PLLDVARRLEEVALDDAYFKEKKLYPNVDFYSGIIMRAIGIPLDMFTVMFAIGRMPGW
                     IANWKEVWEGQSRIYRPRQIYTGPVERSYVPVGQRRAGVAVPGSATVGGQVVCR"
     gene            complement(2014..2196)
                     /locus_tag="PKK95_13230"
     CDS             complement(2014..2196)
                     /locus_tag="PKK95_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19230.1"
                     /translation="MTYNFDPDRWRDDQLARLELERRAGRVSEAEYQAALDEIERRYE
                     SMLARLDGTYQIPRAE"
     gene            complement(2196..2708)
                     /locus_tag="PKK95_13235"
     CDS             complement(2196..2708)
                     /locus_tag="PKK95_13235"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOC19231.1"
                     /translation="MTAEEQILDILRKAYQIEVDGYTFYSMTAEQAIKPAVEELFARL
                     AQDELQHQAFLRDVLGRYQARRTAAFDLPIRIPEGLRAFSDKVFTARFREQAEGAAFE
                     MAALSVGMTLEKNAIAHFSEAARTAGIEEVRKFYQFLADWEHQHLEALQSLHEAVRSD
                     FWDRNAFTPY"
     gene            2997..3893
                     /locus_tag="PKK95_13240"
     CDS             2997..3893
                     /locus_tag="PKK95_13240"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOC19232.1"
                     /translation="MVTTIRELHDLARKGRTRTIAVPWPHDEDVLVSLDKAAEAGLIR
                     PLLAGRRTAIESVRERLGLRPLEEEIVECGSEAEAVEVAVRRVAAGDAQMLMKGLVAT
                     SLFLRGILNKETGLRQKPLLSHVALYETRAPERLVLFSDVAMNIAPDLAQKVQILENA
                     VWLAHRLGIERPRVAAIAAVETVSADMPATLDAALLAKMSDRGQIKGCVVDGPLALDN
                     ALSAEAARHKGITSPVAGAADVLLLPDIEAGNVLYKIMGLLGQRPMAAILVGASAPVV
                     LTSRADSDQTKFNSIALAAAIS"
     gene            4000..4728
                     /locus_tag="PKK95_13245"
     CDS             4000..4728
                     /locus_tag="PKK95_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19233.1"
                     /translation="MAAIPLIEGARLTVISGAFGTGKTEVSINLALALRDGGRGPVTL
                     VDLDIVNLYFRSRQRAYALEALGIRVISSVEGMENADMPALSPAIAACFDRKDGPVVF
                     DLGGSDLGGIVAAYFHRGLSAEPVNHWLVVNPYRPFNETPDAIVEMAGRIEERSRLPI
                     TGMIANPHLIADTTPAVIREGLARVREVTRYPIAALAVMEPNDTPGAFDDLGLPVFTM
                     RKQMKQPWEAGGIMMAGKRGTRCP"
     gene            4719..>4913
                     /locus_tag="PKK95_13250"
     CDS             4719..>4913
                     /locus_tag="PKK95_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC19234.1"
                     /translation="MPMITVSEDRCKGCALCVRVCPTGALREKSAAEDVAKALANPQL
                     DVIAQIAPAVPATVAAEVGLR"
BASE COUNT          777 a         1625 c         1753 g          758 t
ORIGIN      
        1 cgggcgcgag cacctggtcc ggaacctgat ctacccggtg cccgaccccc ggttcccgtt
       61 cctcggcgtc cacttcacgc gaatggtcca cgggggcgtc gaggcggggc ccaacgcggt
      121 gctcgcgttc cggcgggagg ggtacacgcg caccagcttc tcgccgcgcg acacgctgac
      181 cctgttcgag ttcccgggct tctggaggat ggggctgaag tactggcgga tgggtttggg
      241 cgagatgtac cgatcgttca gcaagagggc gttcgtggcg gcgctgcgag agctcgtgcc
      301 ggctctgcgc gacgaggacg tgcgcccgag cggggccggg gtgcgggcgc aggcgctcgc
      361 cccgagcggt gcgctgctcg acgacttcca catcgtggag gccgagcgga tgatccacgt
      421 gctgaacgcc ccgtcccccg cggcgaccgc gtcgatcagc atcggccgct cgatcgccgg
      481 aatggcctcc agggcgttcg cgctaaaatg atcggggttt tggggccagc aaccgttcaa
      541 cgcgaggaaa gggtctatgc ctgagacggc tacgctccag gtgcggggaa gaacctatac
      601 gctgcccgtc gtggtgggga gcgagggcga ggtggcggtc gacatctcga agctgcgcgc
      661 cgaatcgggg gtgatcacgc tcgacgacgg gtacgccaac acgggatcgt gccagtcggc
      721 gatcaccttc atcgacgggg agcgcggcat cctgcggtac cggggcatcc cgatcgagca
      781 aatagccgag cacagcacct tccccgagac cgcgtggctg ctcatctacg gcgagctgcc
      841 caccgccgac cagctcgaat gctggagcaa cctgctgacc cggcacgaga tgctgcacga
      901 ggggctctac aagcacttcg acgggttccc ctcgggcggt caccccatgg ccatcctgtc
      961 ggcgatgatc aacgcgacca gcacgtacaa ccccgacatc atcaggatgg agagcccgga
     1021 gacgttccag agcgcggcgg cgaggctgct gagcaagacg cggacgatcg cggccgccgc
     1081 ctacaagacc tcgatcggtc agccgctggt ctaccccaag ccgcagttcg actacgcctc
     1141 gaacttcctg cacatgatgt tctcgatccc gttcaaggag tacgaaccgg atccggacgt
     1201 cgtcgcggcg ctgtcgctga tcctggtgct ccatgccgac cacgagcaga actgctcgac
     1261 cagcaccgtg cggatggtgg cctcgagcgg cgcgaacctg ttcgcgagct gcgcggccgg
     1321 ggtgtgtgcg ctctgggggc cgctgcacgg gggcgcgaac accgccgtcc tcgagatgct
     1381 gcagtacatc gtggagagcg gcatgtcggt gaaggactac gtcgagaggg tgaaggacag
     1441 gacgtcgaac atcaagctga tgggcttcgg gcaccgggtg tacaagaact acgacccgcg
     1501 ggcgaagatc atcaagcagg cggccgaccg ggtgctgggg aagctgaaga tccacgaccc
     1561 gctgctcgac gtggcgcgcc ggctggagga ggttgccctc gacgacgcct acttcaagga
     1621 gaagaagctc tacccgaacg tggacttcta ttccggcatc atcatgcgcg cgatcggcat
     1681 cccgctggac atgttcaccg tcatgttcgc catcgggcgc atgcccgggt ggatcgccaa
     1741 ctggaaggaa gtctgggagg gacagagccg catctaccgc ccccgccaga tctacaccgg
     1801 tccggtcgag cgcagctacg ttccggtcgg ccagcggcgg gcgggtgtgg ccgttccggg
     1861 ttcggcgacg gtcggcggcc aggtcgtctg ccgctaggga tagtggcgga cagcagacag
     1921 cagacagcgt aggcggccgc ggcagtggca caggcgtccc gcctgtgagc cgcccgtagg
     1981 ggcggccctc gtggccgccc ttccccggcc gtttcactcc gcgcgcggga tctggtaggt
     2041 gccgtcgagg cgcgcgagca tcgactcgta ccggcgctcg atctcgtcga gcgcggcctg
     2101 gtactcggcc tccgacacgc ggccggcgcg gcgctccagc tccagccggg cgagctggtc
     2161 gtcgcgccag cgatccggat cgaagttgta ggtcatcagt acggcgtgaa ggcgttgcgg
     2221 tcccagaaat cgctgcgcac ggcctcgtgc agcgactgca gggcctccag gtgctggtgt
     2281 tcccagtcgg ccaggaactg gtagaacttc ctcacctcct cgatgcccgc cgtcctggcg
     2341 gcttccgaga aatgcgcgat ggcgttcttc tccagcgtca tcccgaccga gagcgcggcc
     2401 atctcgaagg cggccccttc ggcctgctcg cggaagcgcg cggtgaacac cttgtcggag
     2461 aaggcgcgca gcccctcggg gatgcggatc ggcaggtcga aggcggcggt ccgccgggcc
     2521 tggtagcggc cgaggacgtc gcgcaggaac gcctggtgct ggagctcgtc ctgcgccagc
     2581 ctggcgaaca gttcctcgac ggccggcttg atcgcctgct ccgcggtcat tgagtaaaag
     2641 gtgtagccgt ccacctcgat ctggtacgcc ttgcgcagga tgtcgaggat ctgctcttcg
     2701 gccgtcatgc tgtcacctcc cggggcaagg gccccgacgg cctcccatga tatacctggg
     2761 ccgcgtcggc gcggccgccg cgggttcctt gccgccgagc ccggcgtgag gttacactgg
     2821 gctgtctgcg gccttcctgt cctttcgcga acgccccctg ccgctgccac agtctcgagt
     2881 tcttccgtcg atgaacagcg ctgccgcgcc cacggccgtc ccgacctgcc ggtctgccga
     2941 ggggggcggg gcaccgaccc gccgggcgtc tgcgcccggt gacaacggag aggcccgtgg
     3001 tcacgacgat ccgcgagctg cacgacctgg cccgtaaggg acggacacgg acaatcgccg
     3061 tgccctggcc gcacgacgag gacgtgctgg tctcgctgga caaggcggcg gaggcggggc
     3121 tcatccgccc cctgctggcg ggccggcgaa cggccatcga gtcggtgcgc gaacggctcg
     3181 gcctgcgtcc tctcgaggag gagatcgtcg agtgcggcag cgaggccgag gcggtggagg
     3241 tggcggtccg gcgggtggcg gccggcgacg cgcagatgct gatgaagggg ctggtcgcga
     3301 cctcgctctt cctccgcggg atcctcaaca aggaaacggg cctgcgccag aagccgctgc
     3361 tcagccacgt cgccctctac gagacgcggg ccccggaacg cctggtgctc ttcagcgacg
     3421 tcgcgatgaa catcgcgccc gacctcgcgc agaaggtgca gatcctcgaa aacgcggtgt
     3481 ggctggcgca ccgcctcggg atcgagcggc cgcgcgtggc ggcgatcgcc gcggtcgaga
     3541 cggtgagcgc cgacatgccc gcgacgctcg acgcggcgtt gctggccaag atgtccgacc
     3601 gggggcagat caaggggtgc gtggtggacg gcccgctggc gctcgacaac gccctctcgg
     3661 ccgaagccgc ccgtcacaag gggatcacca gccccgtcgc gggggcggcc gacgtgctgc
     3721 tgctgcccga catcgaagcg ggcaacgtgc tctacaagat catgggcctg ctcggccagc
     3781 ggccgatggc cgcgatcctc gtgggcgcga gcgccccggt cgtgctcacc tcgcgtgccg
     3841 acagcgacca gaccaagttc aactcgatcg cgctcgccgc ggccatctca tgatcaggat
     3901 tcgggaatcg ggattcggga ttcgggaatc gggatgcggg aaccgggatt cgggggtcgg
     3961 gacgacgacc cggatttcag gccgtgcccg gggggcggtg tggcggcgat tcccctgatc
     4021 gagggcgcgc ggctgacggt catcagcggg gccttcggca ccggcaagac cgaggtgtcg
     4081 atcaacctgg cgctcgcgct ccgggacggg gggcgcggcc cggtgacgct ggtggacctc
     4141 gacatcgtca acctgtactt ccgctcccgg cagcgcgcgt acgcgctcga ggcgctcggc
     4201 attcgcgtga tctcgagcgt ggagggaatg gagaacgcgg acatgccggc gctctcgccg
     4261 gccatcgcgg cctgcttcga tcgcaaggac gggccggtgg tgttcgacct ggggggcagc
     4321 gacctcgggg ggatcgtcgc cgcctacttc caccgcgggc tctcggccga accggtgaac
     4381 cactggctgg tggtcaaccc ttaccgcccg ttcaacgaga cgcccgacgc gatcgtcgag
     4441 atggccggcc ggatcgagga gcggtcgcgc ctgccgatca cggggatgat tgccaatccc
     4501 cacctcatcg ccgacaccac cccggcggtc atccgcgagg ggctggcgcg cgtgcgcgag
     4561 gtcacccgct acccgatcgc ggcgcttgcc gtgatggagc cgaacgacac gcccggcgcc
     4621 ttcgacgacc tggggctgcc ggtgttcacg atgcggaagc agatgaaaca gccctgggag
     4681 gcaggcggta tcatgatggc cggcaagagg gggacgcgat gcccatgatc acggtcagcg
     4741 aggacaggtg caaggggtgc gcgctctgcg tgcgcgtctg cccgacgggc gcgctccgtg
     4801 aaaaaagcgc tgcggaggat gtggccaaag ctcttgcgaa tccgcaactg gatgtgattg
     4861 cccagattgc tccagccgtc cctgcgaccg ttgcggctga agtcgggctg cgg
//
LOCUS       DAOPQX010000281         4912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18736_length_4912_cov_3.694873, whole genome shotgun sequence.
ACCESSION   DAOPQX010000281 DAOPQX010000000
VERSION     DAOPQX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18736_length_4912_cov_3.694873"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKK95_13255"
     CDS             <1..306
                     /locus_tag="PKK95_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19235.1"
                     /translation="LNQVLPGTNGRLLPLGLYRFLTRGRRIDQIRLALLGVLPEARGK
                     GVYPVLLGEMFRRAVSLGFRRGEASWVLEDNGEVNRVCEQFGGKRYKTYRIYQKACA"
     gene            297..1265
                     /locus_tag="PKK95_13260"
     CDS             297..1265
                     /locus_tag="PKK95_13260"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOC19236.1"
                     /translation="MRVKAAAVTGATGFIGRHLVERLAALGWQVRVIQRPESRAAAPA
                     GIEAVRAPLRADGLAAAFEGTGVVFHLAGRTAAPSAAAFHQANAVAAGEVARACRDAG
                     ARLVHVSSQAAAGPAPANRPATEAGEARPCSAYGISKLAGERIVQDTVGLEWTIARPV
                     AVYGPGDRGFLPLFRLARAGFAVIPGSPAACYTLVHVRDLVEGLIAAAERPEAVREVF
                     FLGHPVLRTARSLAAALGRVAGRPVRTITVPRPVLYAAAVAGDLGARLGLPAALDRSK
                     YRELTSSGFACEVGKARRLLQWEAGIDLEEGFAETVTWYLENGWLR"
     gene            complement(1276..2478)
                     /locus_tag="PKK95_13265"
     CDS             complement(1276..2478)
                     /locus_tag="PKK95_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC19237.1"
                     /translation="MADLLDKLHDRLSQAAALDAGVARPLETVFQRMLGPTVAEIDGR
                     RTLVFGSNNYFGLTFHPEVIAAARDALDRYGSGTTGSRAANGTLAAHRELEEDLARAF
                     GRRHALVFTTGYQANLGMVSGLCAAGDFVLLDNDCHASLYDAARLSGATILGFRHNSA
                     ESLRQRLRRLPQDGKNRLAVVEGLYSIQGDIAPLRELVDVSREEGAYLMVDEAHSIGA
                     YGERGRGCVEAQGVADEVDFVVGTFSKALGGVGGFALSDHDALQGIPLLARPYVFTAS
                     GGAANIAGVRAALRVLMTDRTLAPKLWRAVHRLREGLSALGYRLGPSVSPIVPVTIGD
                     FALTLRAWRALLASGLYVNLILPPACPPDSCLLRFSCSAAHTDAEVDEALELLGRMGR
                     ELGILQGT"
     gene            complement(2496..4283)
                     /locus_tag="PKK95_13270"
     CDS             complement(2496..4283)
                     /locus_tag="PKK95_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HOC19238.1"
                     /translation="MAGWTLPKSLALAAESRASITYMQDDGSERSESYAALYGRAQAI
                     ARHLSENGIGRGHRVALLIPEAEGFVPSLYGVALTGAAVVPLAPPAHLAQLEAALGAW
                     QRMATTAEVTAILTTATLRPMLGSLVAACPTVQEIWAWDEAAANAGPFENQARPDENG
                     LIQFTSGSTSHPKGVVLHHENLGANVEGITGPDGLDVREGDVGVSWLPLFHDMGLIGI
                     VMGGIHARIPLVLMPSLLFLKRPNEWLRTITRHRATLSFAPNFAYELCVRRGAGHANN
                     GIDLSTWRVAGCGSEPIRPETLELFTQTFADRGFRANAYLPCYGLAESTLAATFHALG
                     TPWRFDTVCSKALREEQRAVPCEAGQENAVRLVSCGRPFPGHQVRIADSHDRPLPERS
                     VGEILLRGPSVMQGYYKDARHSAEALRGGWLHTGDLGYVAEGELYVCGRKKDLIIVHG
                     RNYHPQDLEWVASEVDGVRRGNVVAFCTADPNGGERVVLIAESRGRADAEAVAAEIRR
                     RVQEASGLHLYDVRVLPKGAIPKTTSGKVQRGRLKARYESGQPLHVATEPRTVLRHLI
                     SSQWGYTKVAARRLASRFGGRNGRTGLEA"
     gene            complement(4283..4543)
                     /locus_tag="PKK95_13275"
     CDS             complement(4283..4543)
                     /locus_tag="PKK95_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOC19239.1"
                     /translation="MTSPLPNLDERISAVVVRVSRASTPPDRSTELIADLGLDSMHLL
                     ELIAELEDEFGIYIPLNEVPAIKTFGQVVDRVGALLREQGRR"
     gene            complement(4667..>4912)
                     /locus_tag="PKK95_13280"
     CDS             complement(4667..>4912)
                     /locus_tag="PKK95_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19240.1"
                     /translation="TATLIASTLASAAAGLYPRLLPALPGSAHPDLDIYNTASAPGSQ
                     AIALGIYLVGMAIVAVYMVNVYRVWRGRLGDEQVYKI"
BASE COUNT          659 a         1724 c         1844 g          685 t
ORIGIN      
        1 ctgaaccagg tgctgccggg caccaacggc cgcctgctgc cgctcgggct gtaccgcttt
       61 ctgacgcgcg gccggcggat cgaccagatc cgcctcgcgc tgctcggcgt cctgcccgag
      121 gcgcggggga agggggtgta cccggtgctg ctcggcgaga tgttccggcg cgccgtcagc
      181 ctcggcttcc ggcgcggcga agcctcgtgg gtgctcgagg acaacggcga ggtcaaccgc
      241 gtctgcgagc agttcggcgg gaagcggtac aagacctacc ggatctacca gaaggcatgc
      301 gcgtgaaggc cgccgcggtc accggcgcca cggggttcat cggccggcac ctggtcgagc
      361 ggcttgccgc gctcggctgg caggtgcggg tgatccagcg gcccgagagc cgggcggccg
      421 cccccgccgg catcgaggcg gttcgggccc cgctgcgggc ggatgggctg gcggccgcgt
      481 tcgagggcac cggcgtcgtg ttccacctcg cgggccgcac ggcggcgccc agcgccgcgg
      541 ccttccacca ggccaacgcc gtggcggccg gcgaggtggc gcgcgcgtgc cgcgacgcgg
      601 gagcccgtct cgtccacgta tcgagtcagg cggcggcggg cccggcgccg gcaaaccgcc
      661 ccgccaccga ggcgggcgag gcgcgtccct gcagcgccta cgggatctcg aagctggccg
      721 gcgagcggat cgtccaggac actgtgggac tggagtggac catcgcgcgg ccggtggcgg
      781 tctacgggcc cggcgaccgg ggcttcctgc ccctgttccg gctggcacgt gcgggcttcg
      841 ccgtcatccc cggcagcccc gcggcctgct acacgctcgt ccacgtgcgc gatctcgtcg
      901 aagggctgat tgccgcggcc gaacgccccg aagcggtgcg agaggtcttc ttcctcgggc
      961 accccgttct tcgcaccgcg cgctcgctgg ccgccgcgct gggccgcgtg gccggccggc
     1021 cggtgcgaac gatcacggtg ccccggcccg tgctctacgc ggccgcggtc gccggcgacc
     1081 tgggcgcgcg gctcggcctg ccggccgcgc tcgatcgctc caagtaccgc gagctgacct
     1141 ccagcggttt cgcgtgcgag gtggggaagg cgcgccgcct cctgcagtgg gaagccggca
     1201 tcgacctgga ggaggggttc gcggaaacgg tgacgtggta cctggagaac ggctggcttc
     1261 gctgagccgc tgttcctacg ttccctgaag gattcccagc tctcttccca tcctgcccag
     1321 cagctcgagc gcctcgtcca cctcggcgtc ggtgtgggcg gccgagcaac tgaagcggag
     1381 caggcacgag tcgggcgggc aggcgggcgg caggatcagg ttgacgtaga ggcccgaggc
     1441 gagcagcgcg cgccacgcgc gcaaggtcag ggcgaagtcg ccgatggtca cgggtacgat
     1501 gggcgagacc gacggcccga gccggtagcc gagcgcggac agcccttcac gcaggcggtg
     1561 gacggcccgc cacagcttcg gggcgagcgt ccggtcggtc atcagcaccc ggagggccgc
     1621 gcgcacgccg gcgatgttgg cggcgccccc cgacgcggtg aacacgtagg ggcgggcaag
     1681 cagcgggatt ccctgcagcg cgtcgtggtc ggaaagcgcg aagccgccaa cccctcccag
     1741 cgccttcgag aaggtgccga cgacgaagtc cacctcgtcg gcgacgccct gggcctcgac
     1801 gcagccgcgc ccgcgctcgc cgtaggcgcc aatcgagtgc gcctcgtcca ccatcaagta
     1861 cgcgccctcc tcgcgggaca catccaccag ctcgcgcagc ggggcgatgt cgccctggat
     1921 cgagtacagc ccctcgacca ccgcgaggcg gttcttgccg tcctggggca ggcggcggag
     1981 ccgctgccgc aggctttcgg ccgagttgtg ccggaagccg aggatcgtcg cgccgctcag
     2041 gcgggcggcg tcgtacaggc tggcgtggca gtcgttgtcg agcagcacga agtcgccggc
     2101 ggcgcagagg ccgctcacca tcccgaggtt cgcctggtag ccggtcgtga agacgagggc
     2161 gtggcggcgc ccgaaggcgc gggccaggtc ttcctcgagc tcgcggtgcg cggccagcgt
     2221 gccgttggcc gcgcgcgagc cggtcgtgcc gctgccgtag cggtcgagcg cgtccctggc
     2281 cgccgcgatg acctcggggt ggaaggtgag gccgaagtag ttgttcgagc cgaagacgag
     2341 ggtgcggcgc ccgtcgatct cggccacggt ggggccgagc atccgctgga ataccgtttc
     2401 gaggggacgc gcgacgccgg cgtcgagcgc cgccgcctgc gacagccggt cgtgcagctt
     2461 gtcgagcaga tccgccatag gtgagcctgg ctctctcagg cctcgaggcc cgtccgcccg
     2521 ttccggcctc cgaagcgcga cgccagccgg cgggcggcca ctttggtgta gccccactgt
     2581 gaggagatga ggtgccgcag gacggtgcgc ggctcggtgg ccacgtgcag cggctggccc
     2641 gactcgtacc gggccttcag ccgcccgcgc tgcaccttcc cgctggtggt cttggggatg
     2701 gcccccttgg gcagcacccg cacgtcgtag aggtgcagcc cactggcctc ctggacgcgc
     2761 cgccggatct cggcggccac cgcctcggcg tcggcgcggc cgcggctctc ggcaatcagc
     2821 accacgcgct ccccgccgtt cgggtcggcc gtgcagaagg cgaccacgtt gccgcggcgg
     2881 acgccgtcca cctcggaggc cacccactcg aggtcctgcg ggtggtagtt gcggccgtgc
     2941 acgatgatca ggtccttctt ccggccgcag acgtacagct cgccctcggc cacgtagccc
     3001 aggtcgccgg tgtggagcca gccgccgcgc agcgcctcgg ccgagtgccg ggcgtccttg
     3061 tagtagccct gcatgaccga cgggccgcgc agcaggatct cgccgaccga ccgctccggc
     3121 agcggccggt cgtgcgagtc ggcgatgcgc acctggtggc cggggaaggg ccggccgcag
     3181 ctgaccagcc gcaccgcgtt ctcctggccc gcctcgcacg gcaccgcgcg ctgttcctcc
     3241 cggagcgcct tcgagcagac ggtgtcgaag cgccacggcg tgccgagcgc gtggaaggtt
     3301 gccgcgagcg tcgattccgc caggccgtag caggggaggt aggcgttggc gcggaagccc
     3361 cggtcggcga aggtctgggt gaacagctcc agcgtctcgg gacgaatcgg ctcggagccg
     3421 cagccggcca cccgccacgt ggagaggtcg ataccgttgt tggcgtggcc ggcgccgcgc
     3481 cgcacgcaca gctcgtaggc gaagttcggg gcgaagctga gggtggcgcg gtggcgcgtg
     3541 atcgtccgca gccactcgtt cggccgcttg aggaacagca gcgagggcat cagcaccagc
     3601 gggatgcggg cgtggatgcc gcccatcacg atgccgatga gccccatgtc gtggaacagc
     3661 ggcagccacg agacgccgac gtccccctcg cgcacgtcca ggccgtcggg cccggtgatc
     3721 ccctcgacgt tggccccgag gttctcgtgg tggaggacga cacccttggg gtgcgaggtc
     3781 gacccggagg tgaactggat caggccgttc tcgtcgggcc gcgcctggtt ctcgaagggg
     3841 cccgcgttgg cggccgcctc gtcccaggcc cagatctcct gcacggtcgg gcaggcggcc
     3901 accagcgagc cgagcatcgg gcggagggtg gcggtggtca ggatcgccgt cacctcggcg
     3961 gtggtggcca tccgctgcca ggcgccgagc gccgcctcga gctgcgcgag gtgcgcgggc
     4021 ggcgccagcg gcaccaccgc ggcgcccgtc agtgcgacgc cgtagagcga gggcacgaag
     4081 ccctcggcct cgggaatcag cagggccacg cggtggccgc ggccgatccc gttctccgag
     4141 aggtggcgcg cgatggcctg cgcccgcccg tagagcgccg cgtagctctc gctgcgctcg
     4201 ctcccgtcgt cctgcatgta ggtgatgctg gcgcggctct cggccgccag cgccagcgac
     4261 ttcggaaggg tccaccccgc cattaccttc gtccctgttc tcgtaggagc gcgccgacgc
     4321 gatcgacgac ctgcccgaac gtcttgatgg ccggcacctc gttgagcggg atgtagatgc
     4381 cgaactcgtc ctccagctcg gcaatcagct cgagaaggtg catcgaatcg agaccgaggt
     4441 cggcgattag ctcggtcgag cgatccgggg gggtcgaggc gcggctgacg cgcacgacga
     4501 ccgcggaaat ccgttcgtcc aggttgggga gtgggctggt catcggtccg acggagagcc
     4561 ccccggcggg gcaccggcga acaagaatat catagagggg tgcggccggt tcgcgcgtca
     4621 cccgctcacg gccttgctca ccccaagcgg gcgccggggt cggctgctag atcttgtaga
     4681 cctgttcatc ccccagccgg ccccgccaca cccgatacac gttgaccatg tagaccgcca
     4741 cgatcgccat gccgaccagg tagatcccga gggcgatggc ctggctgccg ggggccgagg
     4801 cggtgttgta gatgtcgaga tccgggtggg cgctgccggg cagcgccggc agcaggcgcg
     4861 ggtagaggcc ggcggcggcc gaggccagcg tggaggcaat gagcgtcgcg gt
//
LOCUS       DAOPQX010000282         4900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18802_length_4900_cov_4.383488, whole genome shotgun sequence.
ACCESSION   DAOPQX010000282 DAOPQX010000000
VERSION     DAOPQX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18802_length_4900_cov_4.383488"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKK95_13285"
     CDS             complement(<1..313)
                     /locus_tag="PKK95_13285"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HOC19241.1"
                     /translation="MASTPGQAFDRLVTLVARLRAPDGCPWDREQTLESLRAFVLEEA
                     YEVLEAIDRGDRPALREEIGDFIFEGVLLAQLCAEDGDFTVTDSLTSIADKLVRRHPH
                     VF"
     gene            406..696
                     /locus_tag="PKK95_13290"
     CDS             406..696
                     /locus_tag="PKK95_13290"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOC19242.1"
                     /translation="MSPEPSSSPPITFTLFVLSLAASAEIHLGLMPPPGGDKPGPPNL
                     VEASHLIELLAMLREKTRNNLDESEEKLIEGVLYDLRLRYVEAAGRKRVSEP"
     gene            693..1475
                     /locus_tag="PKK95_13295"
     CDS             693..1475
                     /locus_tag="PKK95_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOC19243.1"
                     /translation="MSGPLARVTFLGTGTSHGVPMIGCDCRTCTSPDPRDSRLRPSVL
                     VDLPSGGRLLVDAAPDLRTQALRHGLRRVDAILFTHAHADHVMGLDETRRFSAIQGGP
                     LPCYGSPDTVEEIRRVFAYAFREGQAGGGVPRLELNGVRGPFEAAGWPVTPVPILHGT
                     LPIYGYRIGRFAYLTDCSEIPEASWPLLEGVETLVVDALRERPHPTHFNVGQALAAIA
                     RVRPRRALLTHISHDLRHEEASRRLPAGVELAYDGLMLEVEA"
     gene            1485..2429
                     /locus_tag="PKK95_13300"
     CDS             1485..2429
                     /locus_tag="PKK95_13300"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOC19244.1"
                     /translation="MPADVIYYPDVQRPPAWHQPVLALGNFDGLHRGHARIVERVVRA
                     AAERNATPLLLTFEPHPTRVVRPDKAPPLLMTLDQKIEVAARAGIQGVAVVRFTPELS
                     AWDPETFVRQVLVEWLRVAEVWVGANFLFGHDRAGNFSRLRSLGARYGFRAEKIDPVR
                     YKDFVVSSTRIRRLVAEGRVDEAGALLGRHYFIDGVVVHGLQRGRLLGFPTANLDTRN
                     ELLPPNGVYATLASVGGIVYPSITNIGVRPTFHQPSATVVEAHLLDREIDLYGVPMRL
                     SFLQRVRDERAFEGVEALKAQIAADCDRARELFRQISV"
     gene            2516..3928
                     /gene="cysS"
                     /locus_tag="PKK95_13305"
     CDS             2516..3928
                     /gene="cysS"
                     /locus_tag="PKK95_13305"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOC19245.1"
                     /translation="MRLYNTLTRREDEFAPADGRTVRMYACGPTVYNRAHIGNFRTFV
                     CCDVLRRTLKHQMGWDVRQVMNFTDVDDRTIAGSQKAGVPLREYTDGWIAAFLEDADA
                     LGLEPVEERPRATDEANLRAMAEIIGSLERAGHTYRSEGSTYYKISTFEQYGRLARLD
                     HEGIKPGARVDADNYDKENARDFVLWKAARPGEPSWDLGFGPGRPGWHIECSAMALRL
                     LGEPPIDLHAGGVDLVFPHHENEIAQSEGATGRPFARFWFHVEHLLIDKEKMSKSLGN
                     QFTIPEVLAQGHRASALRYLLLSTHYRKQLMFTWDSLQQADEALRRLTDFLARLDLVK
                     RDEANPAIAGRVAAARQSFGEMLEADLNTAGAMGVTFDLVRALNTAIDAGEVGTPDVA
                     AIREAFEHFDRILGVISLRRAEEAKAGVPPEEIDRLIAERQAARRRRDFAGADRIRQD
                     LLDRGIVLEDTPAGTRWKRK"
     gene            4006..4389
                     /locus_tag="PKK95_13310"
     CDS             4006..4389
                     /locus_tag="PKK95_13310"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOC19246.1"
                     /translation="MPAPDRIYLGKSGEDAACDELRRRGYAILARGYRTRMGEIDIIA
                     RDGPTLVFVEVKARTTTHRGYPAEAVTLHKQAKVTAMAADYLARCGWHDRPCRFDVVA
                     VLLGERGEPLSVEVFQNAFDAVGWY"
     gene            4446..4520
                     /locus_tag="PKK95_13315"
     tRNA            4446..4520
                     /locus_tag="PKK95_13315"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4478..4480,aa:Gly,seq:gcc)
     gene            4593..4666
                     /locus_tag="PKK95_13320"
     tRNA            4593..4666
                     /locus_tag="PKK95_13320"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4625..4627,aa:Cys,seq:gca)
     gene            complement(4670..>4900)
                     /locus_tag="PKK95_13325"
     CDS             complement(4670..>4900)
                     /locus_tag="PKK95_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19247.1"
                     /translation="LRRTAVRNMVRRGVSERVAMQLAGHKTRSVFDRYNIVSAGDLKT
                     AGQQLSGLTGTIWGQSAGLSPGSESEPKKIAQ"
BASE COUNT          720 a         1738 c         1704 g          738 t
ORIGIN      
        1 cgaagacatg ggggtggcgt cgcacgagct tgtcggcgat cgaggtcagc gagtcggtca
       61 ccgtgaagtc gccgtcctcg gcgcagagct gggccaggag caccccctcg aagatgaagt
      121 cgccgatctc ctcgcgcagc gccggccggt cgccccggtc gatggcctcg agcacctcgt
      181 aggcctcctc gaggacgaag gcgcgcagcg attcgagggt ctgctcgcgg tcccaggggc
      241 acccgtcggg cgcccggaga cgcgccacga gcgtgaccag ccgatcgaac gcctgcccgg
      301 gggtggatgc catcccgcca tgctaccatt tcaagttgcg gccagcgggc cgagcccggg
      361 ccgtgagccg ctggcccgca gccagccagc cgtgagcgtc gagccttgag ccctgagcct
      421 tcttcttccc ctccgatcac cttcacgctg ttcgtcctgt cgctcgcggc aagcgccgag
      481 atccacctcg gcctgatgcc gccgcccgga ggcgacaagc ccgggccgcc gaacctggtc
      541 gaggcctcgc acctgatcga gctgctcgcg atgctgcgcg agaagacgcg caacaacctc
      601 gacgagagcg aggagaagct catcgagggc gtcctgtacg acctgcggct gcggtacgtc
      661 gaggcggccg gccggaagcg ggtgagcgag ccttgagcgg ccccctcgcg cgcgtgacgt
      721 tcctcgggac gggcacctcg cacggggtcc cgatgatcgg ctgcgactgc cggacgtgca
      781 cgtcgcccga cccccgcgat tcccgcctgc ggccctccgt gctggtcgac ctgccgtcgg
      841 gcggacggct gctcgtggac gcggcgcccg acctgcgaac ccaggcgctg cgccacggcc
      901 tccggcgcgt ggacgcgatc ctcttcacgc acgcgcacgc ggaccacgtg atggggctcg
      961 acgagacgcg ccgcttctcg gcgatccagg gggggccgct cccgtgctac ggcagccccg
     1021 acaccgtcga ggagatccgt cgcgtcttcg catacgcctt ccgggagggg caggccggag
     1081 gaggggttcc gcgcctcgag ctgaatggcg tgcgcgggcc gttcgaggcc gccgggtggc
     1141 cggtgacgcc ggttccgatc ctccacggca cgctgccgat ctacggctac cgcatcggcc
     1201 gcttcgccta cctcaccgac tgcagcgaga tcccggaggc gtcgtggccg ctgctcgagg
     1261 gggtcgagac gctggtggtg gacgccctgc gcgagcggcc gcaccccacg cacttcaacg
     1321 tcgggcaggc gctggccgcg attgcccgcg taaggccgcg gcgcgcgctg ctgacccaca
     1381 tcagccacga cctgcgccac gaggaggcct cgcgccgcct gccggccggc gtcgagctgg
     1441 cttatgatgg tttgatgctc gaggtcgaag cctagcccac cctcatgccg gccgacgtca
     1501 tctactaccc cgacgtgcag cggccgcccg cctggcacca gccggtgctg gccctgggga
     1561 acttcgacgg cctgcaccgc ggccacgcgc ggatcgtcga gcgcgtggtc cgcgcggccg
     1621 ccgagcgcaa cgcgacgccg ctgctgctca ccttcgagcc tcacccgacg cgggtcgtcc
     1681 ggcccgacaa ggcgccgccc ctgctgatga cgctcgatca gaagatcgag gtggccgccc
     1741 gcgccggcat ccagggggtg gccgtcgtcc gcttcacgcc ggagctgtcg gcgtgggacc
     1801 ccgagacctt cgtgcggcag gtgctggtgg agtggctgcg ggtggccgag gtgtgggtgg
     1861 gcgccaactt cctcttcggg cacgaccgcg ccggcaactt ctcgcgcctg cgctcgctcg
     1921 gggcgcgcta cggcttccgg gccgagaaga tcgacccggt gcgctacaag gacttcgtgg
     1981 tgagcagcac ccgcatccgg cggctggtgg ccgagggacg cgtggacgag gccggggcgc
     2041 tgctcggccg ccactacttc atcgacgggg tcgtcgtgca cgggctgcag cgaggccgcc
     2101 tgctgggttt cccgacggcc aacctcgaca cccgcaacga gctgctgccg cccaacgggg
     2161 tgtacgcgac gctggcgtcg gttggcggca tcgtctatcc gtcgatcacc aacatcggcg
     2221 tgcggccgac cttccaccag ccgtcggcga ccgtggtgga ggcgcacctg ctggaccgcg
     2281 agatcgacct gtacggcgtc ccgatgcgcc tgtcgttcct gcagcgtgtc cgcgacgagc
     2341 gggcattcga aggggtggag gcgctcaagg cgcagattgc cgccgactgc gaccgcgccc
     2401 gcgagctgtt ccggcagatc tcggtatagg ggcagggatc gggatcttcc gactcccgac
     2461 ttcgccgact cgacttcgcg atttcgtgac ttcgtgaccc gtggactgac agaagatgcg
     2521 gctgtacaac accctcaccc gccgtgaaga cgagttcgcg cctgccgacg ggcggacggt
     2581 gcggatgtac gcctgcggcc ccaccgtcta caaccgggcg cacatcggca acttccgcac
     2641 cttcgtctgc tgcgacgtgc tccgccgcac gctgaagcac cagatgggct gggacgtccg
     2701 ccaggtgatg aacttcaccg acgtcgacga ccggacgatt gccggctcgc agaaggccgg
     2761 tgtgccgctc cgcgagtaca ccgacggctg gattgccgcg ttcctcgagg acgccgacgc
     2821 gctggggctc gagcccgtgg aggagcgccc gcgtgccacc gacgaagcga acctgcgcgc
     2881 gatggcggag atcatcgggt cgctcgagcg ggccggccac acctaccgca gcgagggctc
     2941 cacctactac aagatctcga ccttcgagca gtacgggcgc ctggcccgcc tcgaccacga
     3001 ggggatcaag cccggggcgc gggtggacgc cgacaactac gacaaggaga acgcccgcga
     3061 cttcgtgctg tggaaggcgg cgcggccggg cgagccgagc tgggacctcg gcttcggccc
     3121 gggccgcccc gggtggcaca tcgagtgctc ggcgatggcg ctgcggctgc tgggcgagcc
     3181 gcccatcgac ctgcacgccg gcggcgtcga cctcgtcttc ccgcaccacg agaacgagat
     3241 cgcgcagagc gagggagcga ccgggcgccc gttcgcccgc ttctggttcc acgtcgagca
     3301 cctgctgatc gacaaggaga agatgtccaa gtcgctcggc aaccagttca ccatccccga
     3361 ggtgctggcg cagggccacc gggcctcggc gctgcgctac ctgctgctct cgacccacta
     3421 ccggaagcag ctgatgttca cctgggacag cctgcagcag gccgacgagg cgctgcggcg
     3481 gctgaccgac ttcctcgcgc gcctcgacct ggtgaagcgc gacgaggcga acccggcgat
     3541 cgccgggcgc gtggccgccg ccaggcagtc gttcggcgag atgctcgagg ccgacctgaa
     3601 cacggccggc gcgatgggcg tgacgttcga cctggtgcgc gccctgaaca cggcgatcga
     3661 tgccggcgag gtgggtactc cagacgtcgc ggcaatccgg gaggccttcg agcacttcga
     3721 tcggatcctc ggcgtcatct cccttcggcg cgccgaggag gcgaaggccg gcgtgccgcc
     3781 cgaagagatc gatcgcctga tcgccgagcg gcaggccgcg cgccgccgcc gcgacttcgc
     3841 cggcgccgac cgcatccggc aggacctgct cgatcgcggc atcgtcctcg aggacacccc
     3901 cgccggcacg cgctggaagc ggaagtaggg gcgacccgcc gaagcctcgt ggcgaaggcg
     3961 agtggtcgcc cgcccggctc gtcctttgca cccctccagg ggcgtatgcc cgcccctgat
     4021 cggatctacc ttggaaaatc gggtgaagat gcggcctgcg acgagctgcg ccggcgcggg
     4081 tacgcgattc ttgcccgggg ctaccgcacc cgcatgggtg aaatcgacat catcgcccgc
     4141 gacggcccga cgctggtctt cgtcgaggtg aaggcgcgca cgaccacgca ccgggggtac
     4201 ccggcggagg ccgtcacgct gcacaagcag gcgaaggtca ccgccatggc ggccgactac
     4261 ctggcgcgct gcggctggca cgatcgcccg tgccggttcg acgttgtcgc ggtgctgctc
     4321 ggcgagcgcg gcgagccgct gtcggtggag gtgttccaga acgccttcga cgcggtgggg
     4381 tggtactgac caggccccga cccttgattc ggctgggccg gtatggtact ttgagtgttc
     4441 gctgagcggg aataactcag tggtagagtg cgaccttgcc aaggtcgaag tcgcgggttc
     4501 gaatcccgtt tcccgctcca tccttcctct tgcagcgcgg gggcggtccc gccggttccc
     4561 gtgcgggtcg atcccagcga gcgcgacagg acggcgccgt agccaagtgg taaggcagag
     4621 gtctgcaaaa cctccatctc cggttcaaat ccggacggcg cctccaaatt cactgagcaa
     4681 tcttcttcgg ttcgctttcg cttcccggcg aaagtccggc cgactgtccc caaattgtcc
     4741 ccgtgagccc ggagagctgc tgtccggccg tcttcaggtc gccggcgctg acgatgttgt
     4801 agcggtcgaa cacgcttcgc gtcttgtgcc cggcgagctg catcgcgacg cgttcggaaa
     4861 cgccccgcct caccatgttg cgaaccgccg tccgtcgcag
//
LOCUS       DAOPQX010000283         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18832_length_4894_cov_2.966729, whole genome shotgun sequence.
ACCESSION   DAOPQX010000283 DAOPQX010000000
VERSION     DAOPQX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18832_length_4894_cov_2.966729"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PKK95_13330"
     CDS             complement(<1..471)
                     /locus_tag="PKK95_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOC19248.1"
                     /translation="MILAAASAAAPFEAGVARADITPREPIRMGGYAARTRPSEGVAM
                     PLWAKALALRDARGGRVVIVTLDLLRAPRILTDAVANEVEQRYGLERSQVLFNCSHNH
                     SGPALWEDDPYPSLGADDYAVCRRYMEGLTKEVTRLVGAALDDLAPATLTVSEGS"
     gene            complement(542..1294)
                     /locus_tag="PKK95_13335"
     CDS             complement(542..1294)
                     /locus_tag="PKK95_13335"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC19249.1"
                     /translation="MPRAASGGARRRSGGRDAVPAYRRIQGDVRARIESGTLAPGDRL
                     ESERQLAGRYGVSLMTARHAMNVLERDGLVERRLGSGTFVAPPRIHFNRLQSFSEQMA
                     ARGERARSRIVGIQVLSDEIEAAARLGLPPGTRLVRLERVRLGGSEPFAFETTFLPDR
                     EFPGLARRVRASGSLFETLRRDYGLEPEYAEEEVDALSADARLAHLLDIPRGSPLLRI
                     RQVLFAAAGRRLLYDVGIYRSERHSLMIRRSR"
     gene            complement(1303..2046)
                     /locus_tag="PKK95_13340"
     CDS             complement(1303..2046)
                     /locus_tag="PKK95_13340"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOC19250.1"
                     /translation="MASEGAEVAGGKAAADYRAEVDRILDRISATQAEPLEQAAVLIA
                     DALDRGGVVYAFGAGHSQSVALEFYYRAGGLAPCDVIEERTFGRAERLSGYAAVLLEP
                     YPLGENDLLVAASNSGRNPLPVEMAIEARRRGARVVGITSLAHSRTIAARPPLTHRLF
                     EVCDVVIDNCTPPGDAAVELPAAGGVRVGGLSTLAGVFIAQTLVCRASELIAARGHAV
                     PVFCSMNLDGGDERNRALLAGVKGRVRGI"
     gene            complement(2027..2974)
                     /locus_tag="PKK95_13345"
     CDS             complement(2027..2974)
                     /locus_tag="PKK95_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC19251.1"
                     /translation="MYSAGIDFGGSSTKLALVGRDGRVHAFRRVAGAPGGDLDRVFAA
                     VRETLAAMAAEVSLEFPPPGGAGIGVAGIVDVAAGRVEITGALGMEDCDLRGPAERAL
                     GCPVAVESDSNAGALADLYFGAARGADDVIYVSWGTGIGAGLVVGKRLLRSRGKAVGE
                     IGHSRVVLEDGRRCYCGCRGCLEAEAGGAAIAARGAAVMGRQVTVLDVALAAGNDPAC
                     VAILEQAAACLARGLSPAIVLLNTQAVVLGGGVSRVLERLEARRAFDGELERSLPLFA
                     RRTLTVSLSAFGDRAGAVGAALLPRHLGGEEPGHGERGC"
     gene            3201..4667
                     /locus_tag="PKK95_13350"
     CDS             3201..4667
                     /locus_tag="PKK95_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19252.1"
                     /translation="MRSTARVSIVSFVLAAAALCVPGRPAASGGPAHGPAVSAAESRA
                     VWIWGGTVRSEGPEAVAEAMKAAGVTDAILLVKGTAGTVGYPSAIAPHAATVDTLGPF
                     TDACHARGIRVHAWLNYHQDDAFGARTKNAYSIWHVRTMPAPPSSVNDGRICPLRAQE
                     EYNAYFLSIVDEILERYPVDGIHLDYIRYPHAVYCFCPAHQAKAAAEGIDFTRVRTLL
                     NQTFYAPADQKTYFNAIASGDPDAVKWAAMRSSEIEGVVRTVREAIDRHNGRRPAGRR
                     PIVRLSAAFMPEGALPDLVTDLARSDQFALAHYAQDYGRLSKWLSFVAPMAYHKDYGR
                     PASWVGQVVAGAAAKAGPNGSAVWAGIQTYSVPPQDVVDALGAARLNGSSGFALFRYA
                     TTTAQAWEALKPTIADIREATAAAAEAGWIDANLLNSFEQKLAAVEASLEAGRDGAAA
                     NQLGAFVNELKAQGGKGVDARAARALAADALWVSDLLR"
     gene            complement(4709..>4894)
                     /locus_tag="PKK95_13355"
     CDS             complement(4709..>4894)
                     /locus_tag="PKK95_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19253.1"
                     /translation="PAEGTSIGNILVQAITAGVIGSLEEARAVVRRSFEPARYEPRDR
                     EPWDRAYRRFSELTAVG"
BASE COUNT          740 a         1844 c         1692 g          618 t
ORIGIN      
        1 cgagccctcc gacacggtca gcgtggcggg agccaggtcg tccagggccg cgccgaccaa
       61 gcgggtcacc tccttcgtca gaccctccat gtaacgcctg cacacggcgt agtcgtcggc
      121 cccgagcgac gggtacggat cgtcctccca caaggcaggc ccggagtgat tgtgcgagca
      181 gttgaagagg acctgcgagc gctcgagccc gtagcgctgc tccacttcgt tcgcgaccgc
      241 gtcggtgagg atccgcggcg cgcgcagcag atcgagcgtg acgatgacga cgcgtccgcc
      301 gcgggcgtcg cgcagagcca gggccttggc ccagagcggc atcgccaccc cctcggaggg
      361 gcgggtgcgc gcggcgtagc cgcccatccg gatcggctcg cgcggggtga tgtccgcgcg
      421 ggcgacgccg gcttcgaacg gagcggcggc cgaggcggcc gcgaggatca ttggcaatag
      481 ccatgtcatg ggacgagtcc tggcgcgacg cgcggcctgc accgcgaagc aggcccgcac
      541 gtcagcggga tcggcgaatc atcagcgagt gccgctcgga acggtagatg cccacatcgt
      601 acagcagccg gcggccggcg gcggcgaaca gcacctggcg gatccgcagc agcgggctgc
      661 cccgcgggat gtcgagcagg tgcgcgaggc gggcatcggc cgacagggcg tccacctcct
      721 cctccgcgta ctcgggctcg aggccgtagt cgcgccgcag cgtctcgaac agcgaaccgc
      781 tcgcccgcac tcgccgggcg aggccgggga actcgcgatc cgggaggaag gtggtctcga
      841 aggcaaacgg ctcggatccc ccgaggcgca cccgctcgag gcgtacgagg cgggtacccg
      901 gcggcaggcc gagccgggcc gccgcctcga tctcgtctga caacacctgg atgccgacga
      961 tccgcgaccg ggcgcgctcg ccgcgcgccg ccatctgctc ggagaagctc tggagccggt
     1021 tgaagtgaat gcgcgggggc gccacgaacg tccccgaccc gagccgccgc tcgaccagcc
     1081 cgtcccgctc caggacgttc atggcgtggc gggcggtcat caggctcacg ccgtagcggc
     1141 cggccagctg ccgctccgac tcgaggcggt cgcccggggc cagggtgccg ctctcgatgc
     1201 gggcccggac gtcgccctgg atccgcctgt acgccggcac cgcgtcgcgg ccgcccgaac
     1261 gccgccgcgc ccccccgctc gccgctctcg gcatgccgtc cctcaaatgc cgcgcacgcg
     1321 gcccttcacc ccagcgagca gagcgcggtt gcgctcgtcg cccccgtcca ggttcatgct
     1381 gcagaagacc ggcaccgcgt gcccccgcgc ggcgatcagc tccgacgccc ggcacacgag
     1441 cgtctgtgcg atgaagacgc ccgccagcgt cgaaaggcct ccgacgcgca cgccccccgc
     1501 ggccggcagc tccaccgcgg cgtctcccgg cggcgtgcaa ttgtcgatga cgacgtcgca
     1561 cacctcgaag aggcggtgcg tcaacggcgg ccgtgcggcg atcgtccggc tgtgcgccag
     1621 ggaggtgatc ccgaccaccc gcgcgccgcg gcgccgcgct tcgatggcca tctcgacggg
     1681 cagggggttg cggcccgagt tcgacgcggc gacgagcagg tcgttttctc ccagcggata
     1741 gggctccagc aggacggcgg catacccgga gaggcgttcg gcacgcccga aggtccgctc
     1801 ctcgatcacg tcgcacgggg cgaggccgcc cgctcgatag tagaactcga gcgcgacgga
     1861 ctgcgaatgc ccggcgccga aggcgtacac gacccctccc cgatccaggg cgtcggctat
     1921 caggaccgcc gcctgctcca acggctccgc ctgcgtggcg gaaatccggt ccaggattcg
     1981 atccacctcg gcgcgatagt cggccgcggc cttcccgcct gccacttcag caccctcgct
     2041 cgccatgtcc aggctcctct cctccgagat gccgcggcaa cagcgcggcc ccgactgccc
     2101 cggccctgtc gccgaaggcc gagagcgaca ccgtgagagt gcgccgggcg aagagcggga
     2161 gcgatcgctc cagctcgcca tcgaaggcgc ggcgcgcctc cagtcgctcg agcacccggc
     2221 tcacgccgcc gcccagaacg accgcctgcg tgttcagcag gacgatggcc ggcgacaggc
     2281 cccgcgccag gcaggcggcc gcctgctcga ggatcgcgac gcaggcggga tcgtttcccg
     2341 cggccaacgc cacgtcgagg accgtcacct ggcggcccat caccgccgcg ccgcgcgcgg
     2401 cgatcgccgc ccccccagcc tccgcctcga gacagccgcg gcatccgcag tagcagcggc
     2461 gcccgtcctc gagcacgacg cggctgtggc caatctcgcc gaccgccttg ccgcggcttc
     2521 gcagcaaccg cttccccacc accagtcctg cgccgatccc ggttccccac gaaacgtaga
     2581 tcacgtcgtc ggcgccacgt gcggcgccga agtacaggtc ggcaagcgcg ccggcgttcg
     2641 agtcgctctc caccgccacg gggcagccaa gcgcccgctc ggccggcccc ctgagatcgc
     2701 agtcctccat cccgagcgca ccggtgatct ccacgcgccc cgccgcgacg tccacgatgc
     2761 cggcgacacc aatccccgcg ccgccaggcg gcggaaattc cagcgagacc tcggcggcca
     2821 tggcggccag cgtttcccgc accgcggcga acacgcggtc gaggtcgcct cccggcgcgc
     2881 cggcgacgcg ccggaacgcg tgcacgcggc cgtcgcggcc cacgagggcg agcttggtcg
     2941 aggagccgcc gaagtcgatg cccgcgctgt acacgtcgcg ccccgctccc gttcgacacg
     3001 agaagtggga cctggcccgc cgctcaccga cgggggacgg accggccccg agcccgccgc
     3061 ccacaaacgc tatatagcgt atcccgccgc gcccaggggc gcaagaggcc gtgtccaaaa
     3121 tcttcttgac accgcagacg aagccgagta gatctgtggc cgcaattgtt atatagcact
     3181 tgcaggaagg gagccgggca atgagaagca ccgcgagagt ctcgatcgtc tccttcgtgc
     3241 tggccgccgc ggccctgtgc gtcccgggcc gcccggccgc gtcgggcggg cccgcacacg
     3301 gtcccgccgt ctccgctgcc gagtcgcggg ctgtctggat ctggggcggc accgttcgca
     3361 gcgaggggcc ggaagcggtg gccgaggcca tgaaggcggc aggcgtcacc gacgcgatcc
     3421 tgctcgtcaa gggtaccgcg ggcaccgtgg gttacccgag cgcgatcgcg ccgcacgcgg
     3481 caaccgtcga taccctcggc ccgttcaccg acgcctgcca cgcgcgcggc atccgggttc
     3541 acgcctggct gaactaccac caggacgatg cgttcggcgc caggacgaag aacgcctatt
     3601 ccatctggca cgtccgcacc atgcccgccc cgccctcgtc ggtgaacgac ggccggatct
     3661 gtcccctgcg ggcgcaggag gagtacaacg cctacttcct ctcgatcgtc gacgagatcc
     3721 tcgaacgcta cccggtggac gggattcacc tcgattacat ccggtacccc cacgcggtct
     3781 actgcttctg cccggcccac caggcgaagg cggcggccga gggcatcgac ttcaccaggg
     3841 tgcggacgct cctcaaccag acgttctacg cgcccgcgga ccagaagacc tacttcaacg
     3901 cgatcgcctc gggcgacccg gacgcggtca agtgggccgc gatgcgttct tcagaaatcg
     3961 agggcgtcgt ccggacggtg cgcgaggcga tcgaccggca caacgggcgc cgcccggccg
     4021 gccggcgccc aatcgtccgg ctgtcggctg ccttcatgcc ggaaggggcc ctgcccgacc
     4081 tggtgaccga cctcgcccgc agcgatcagt tcgcgctcgc ccactacgcg caggactacg
     4141 gccggctgtc gaagtggctc tcgttcgtgg cgccgatggc ctatcacaag gactacggca
     4201 ggccggcgtc gtgggtcggg caggtggtcg cgggggcggc ggcgaaggcc ggcccgaacg
     4261 gcagcgcggt gtgggcggga atccagacct acagcgtccc gccccaggac gtggtggacg
     4321 cgctcggggc cgcccgcctg aacggctcgt cgggcttcgc gctcttcagg tacgccacga
     4381 ccacggcgca ggcgtgggag gcgctcaagc ccaccattgc ggacatccgc gaggccaccg
     4441 cggcagccgc ggaggcggga tggatcgacg ccaacctgct gaacagcttc gagcagaagt
     4501 tggcggctgt cgaggcgtcg ctcgaggccg ggcgcgacgg ggccgcagcg aaccagcttg
     4561 gcgcgttcgt caacgagctg aaggcccagg gcggaaaagg cgtggacgcc cgcgcggccc
     4621 gcgccctggc ggccgacgcc ctctgggtct cagatctgct tcgctgagct ggcgaagctc
     4681 gcagccgcgg gctgacggcc ttggccgctc agcccacggc ggtcaactcc gagaaccgcc
     4741 ggtacgcgcg gtcccagggc tcgcggtcgc gcggctcgta ccgggcgggc tcgaacgagc
     4801 ggcggacaac ggcccgcgcc tcctcgagcg acccaatcac gccggccgtg atggcctgga
     4861 cgaggatgtt cccgatcgag gtgccctcgg ccgg
//
LOCUS       DAOPQX010000284         4880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_18893_length_4880_cov_5.081036, whole genome shotgun sequence.
ACCESSION   DAOPQX010000284 DAOPQX010000000
VERSION     DAOPQX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18893_length_4880_cov_5.081036"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..537)
                     /locus_tag="PKK95_13360"
     CDS             complement(73..537)
                     /locus_tag="PKK95_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOC19254.1"
                     /translation="MSWSKLLVVALLTAAPPLSGPALEREARQIETMLIAPCCWNQPV
                     SVHQSPASDQVKGEIRSLLAAGRSRQDVLDDFAARYGTRILAEPPAVGFTRFLYVLPW
                     IVFAGSAVGLTWLVRRMSAKHPVEAPEPERAAPAGAAAGYEQRLDDELRDLD"
     gene            complement(554..1114)
                     /locus_tag="PKK95_13365"
     CDS             complement(554..1114)
                     /locus_tag="PKK95_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19255.1"
                     /translation="MKHAILVGIAVVGLAASGYAQAPQGQGQRARPQAGQAWGPHFVD
                     ADGDGVCDKAGTMQGRRGMRGRGMGAGLSFGRGGLSLVDVTAKVTGQDRAAVVASLRE
                     GKTLAQVAEAAGKSAADVKQAALDARKAKLDEAVAAGRLTAEEADAMMTLMDARIDRQ
                     LTSDWPAGRRRPGFGRRTAPQAPIGR"
     gene            complement(1334..2698)
                     /locus_tag="PKK95_13370"
     CDS             complement(1334..2698)
                     /locus_tag="PKK95_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC19256.1"
                     /translation="MPDQVILLVDDEPAQRLVLAGFLRKRGFDVVQAGTSEDALAELS
                     ARTIDLVLTDLRMPGASGIDLLEGSRRINPEVPVIVTTAFGTVASAVEAMKRGAADYL
                     TKPIDLEELDVLIARTLERRALVAENRELRRQLESRHRLAGLETANARMAEAINTAAR
                     AAGSRATILIRGESGTGKELLARAVHYASPRASGPLVAVNVAALPDTLLESELFGHER
                     GAFTGADREHRGRFELADGGSLFLDEIGDLPRTTQVKLLRVLQEQAFERLGGTRTLRV
                     DVRVLAATNRDLEGMVARGEFRDDLYYRLNVVSIEVPPLRERREDIPALVEHFLKRFA
                     DENQSPSKEISREAMDLLLKYPYPGNVRELENLVHRAVVLSRGRTITAADLPVHLARV
                     GRAGTAATDTPFVERVSEFERSLIVEALERAGGVQTRAARELGMSERHLRYKLKKYKL
                     TADS"
     gene            complement(2691..4499)
                     /locus_tag="PKK95_13375"
     CDS             complement(2691..4499)
                     /locus_tag="PKK95_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC19257.1"
                     /translation="MKPTLSTARIVAATLLAAAGLVFVGWFDYSATRRELLGLLRGQG
                     AALRQTVAAAARSNQAAGAVASTQVIERLLDNARLLAELDRKGSLTGDFLAETAERNR
                     LFRVAVFGPDGAREGGGVAGRAGSPGWRRAPAGRRSAPRAGERQEVPPAPGTSMAPGG
                     TAPGGGTGMGPGAAFIEPILSGRQREVVGELRPAGRRAGSRVAAAVRRENGGAILVNA
                     DASEIESLLRQVSLDRLIESIAATTPQLAYIVVDRDGLRHGHGELPGDLPEPAVAGAG
                     LAPGVEEREREAAGRPVLELSSALDLGEGEIGALRLGLRLDEVRAAERRMFTRMAVSL
                     AAALLLSVVALGAIWLRQAYVTLSEKHARAEAALRRRDRLSAMGELASTVAHEVRNPL
                     NAIAMSAQRLRREFAPAAATEADRAEAAELLSVMEGETGRINDIVQQFLAFARPPRLA
                     PGPANLAVETRALVDAARPLADNRGVTLEADVASAGEAVFDARQLRQAIDNLVRNAIE
                     ATPAGGRVRVVARTGSRGHTVEVSDTGSGIAADDLPKIFDLYYTTKPEGTGVGLAVTQ
                     QIVSAHGGTIEVESAPGAGTTMIVRLPAEVDGGTDA"
     gene            complement(4544..>4880)
                     /locus_tag="PKK95_13380"
     CDS             complement(4544..>4880)
                     /locus_tag="PKK95_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOC19258.1"
                     /translation="KRYYGHGAGVTVVDDYVAHEWAFIPHFYRAFYVYQYATSFTASA
                     ALAERALAGDQETIRKYLELISAGGSDYPIALLRRAGVDMTTREPLDLTMKQMNRVMD
                     EMEKLLEKP"
BASE COUNT          672 a         1836 c         1659 g          713 t
ORIGIN      
        1 atagttgtcc cctcgagaat gcggccagcg tatttggcgg gtcacaaggg cggccagaac
       61 ggccgcccga ggtcagtcca ggtccctgag ctcgtcgtcg aggcgctgtt cgtaaccagc
      121 ggctgcaccg gcaggggcgg cacgctcggg ctcgggggcc tccaccgggt gcttcgcgct
      181 catgcggcga accagccacg tcagcccgac ggcactgccg gcgaacacga tccaggggag
      241 gacgtacagg aagcgggtga agccgacggc ggggggttcg gcgaggatcc gcgtgccgta
      301 ccgggcggcg aagtcgtcga gcacgtcctg gcgcgaacgg cccgcggcca gcagcgaccg
      361 gatctcgccc ttgacctggt cggaggcggg agactggtgc acggaaaccg gctggttcca
      421 gcagcagggc gcgatcagca tcgtctcgat ctggcgcgcc tctcgctcga gcgcggggcc
      481 ggagagcggc ggcgccgcgg tcagcagcgc caccaccagg agcttggacc agctcatcga
      541 accgccttgg ccgtcatcga ccgatcggcg cctgcggcgc cgtccgccgg ccgaagcccg
      601 ggcgccggcg tcccgccggc cagtcggagg tcagctgccg atcgatcctc gcatccatca
      661 gcgtcatcat ggcgtccgcc tcttccgcgg tcaagcgccc ggcagcgacc gcctcgtcga
      721 gcttcgcctt acgggcgtcg agagccgcct gcttcacgtc ggcggccgac ttgcccgccg
      781 cctcggcaac ctgcgccagc gtcttcccct cgcgcagcga ggccaccacc gcggcccggt
      841 cctgccccgt caccttcgcg gtgacgtcca cgagcgagag cccgccgcgg ccgaaggaca
      901 ggccggcgcc catgccgcgc ccgcgcatgc cccgccgccc ctgcatcgtg ccggccttgt
      961 cgcacacgcc gtcgccgtcc gcgtcgacga agtgcggccc ccaggcctgt cccgcctgcg
     1021 gacgcgcccg ctggccctgg ccctgcggcg cctgcgcata gccggacgcc gcgaggccga
     1081 ccaccgcaat gccaaccagg attgcatgct tcatctcttc attctccctg agtgtcgttt
     1141 gcaccgtgtc gtgctattcg acacaagggc aactccggtg ccagagaggg acgggctgat
     1201 atcgcgccaa ttatcgcggt ttccggaagg atgacgagca gccctgcccg gcggcgcgac
     1261 cacccggtcg agagattcga cgaattggtc gattgatacg ccagccgcca gccactcccg
     1321 agtctctgtc ctgtcagctg tccgccgtca gcttgtattt ctttagcttg taccgcaggt
     1381 gccgctcgct catgcccagc tcgcgcgcgg ctcgcgtttg cacgccgccc gcccgttcga
     1441 gcgcctccac aatgagtgac cgctcgaact ccgacacacg ctcgacaaac ggggtgtcgg
     1501 tcgcggccgt cccggcccgg ccgacccgcg ccaggtgcac tggcagatcg gcagcggtga
     1561 tggtgcggcc ccgcgagagg acgaccgccc ggtgcacgag gttctccagc tcgcggacgt
     1621 ttcccgggta gggatatttc agcaggaggt ccatcgcctc acgcgaaatc tccttcgacg
     1681 gcgactggtt ctcgtctgcg aaccgtttca ggaagtgctc gacgagggcc gggatgtcct
     1741 cccgccgctc gcgcagcggc ggcacctcga tcgagacgac gttcagccgg tagtacaggt
     1801 cgtcgcggaa ctctccccgg gccaccatcc cctcgaggtc gcggttggtc gctgcaagaa
     1861 cccgcacgtc cacccggagg gtccgcgtgc cgccgagccg ctcgaaggcc tgctcctgga
     1921 gcacccgtag cagcttcacc tgcgtggttc tcggcaagtc gccgatctcg tcgaggaaca
     1981 gcgagccgcc gtcggccagc tcgaagcggc cccggtgctc gcggtcggcc ccggtgaagg
     2041 cgccccgctc gtggccgaac agctcggatt cgagcagggt atcgggcaac gccgcaacat
     2101 tgacggccac cagcggcccg ctcgcgcgcg ggcttgcgta gtggaccgcc cgggccagca
     2161 gctccttccc cgtgccgctc tcgccgcgaa tcagaatggt ggcgcggctt ccggcggcac
     2221 gcgcggcggt gttgatggcc tcggccatcc gcgcgttcgc cgtctcgagg cccgccaggc
     2281 ggtgccgcga ctcgagctgc cgcctcagtt cgcggttctc ggccacgagc gcccggcgct
     2341 cgagcgtccg ggcgatcagg acgtccagtt cctccaggtc gatcggcttg gtgaggtagt
     2401 cggccgcccc gcgcttcatc gcctccacgg cgctcgccac ggtgccgaag gccgtcgtca
     2461 cgatcaccgg gacctcgggg ttgatgcggc gcgacccttc gagcaggtct atcccgctcg
     2521 cacccggcat gcggagatcg gtcagcacga ggtcgatggt gcgggcgctc aattcggcga
     2581 gcgcatcctc ggacgtgccc gcctggacga cgtcgaagcc gcgcttccgg aggaagccgg
     2641 cgagcaccag gcgctgcgca ggctcgtcgt ccaccaggag gatcacctgg tcaggcatcg
     2701 gtccctccgt cgacctccgc cggcagcctg acaatcatcg tggtgccggc ccccggcgcc
     2761 gactccacct cgatcgtccc gccgtgggcc gacacgatct gctgcgtcac cgcgaggccc
     2821 acgcccgtcc cctctggctt ggtcgtgtag tacaggtcga agatcttcgg caggtcgtcg
     2881 gccgcgatgc cgctgcccgt gtcgcttacc tccacggtgt gcccgcgcga gcccgtcctg
     2941 gcgacgaccc gcacccggcc gccggccggc gtcgcctcga tggcgttgcg cacgaggttg
     3001 tcgattgcct gccgcagctg gcgcgcatcg aacacggctt cgcccgccga cgcgacgtcc
     3061 gcctcgagcg tcacgccgcg gttgtcggcg agcgggcgcg ccgcgtcgac aagcgcccgt
     3121 gtctcgacgg ccaggttcgc cgggccaggg gcgagacgcg gcgggcgggc gaacgcgagg
     3181 aactgctgga cgatgtcgtt gatccggccg gtctcccctt ccatcaccga cagcagctcg
     3241 gcggcctcgg ctcgatcggc ctcggtcgcg gcggccggcg cgaactcgcg ccgcagccgc
     3301 tgcgcgctca tcgcgattgc gttgagcggg ttgcgcacct cgtgcgcgac ggtcgaggcc
     3361 agctccccca tcgcgctcag ccggtcgcgc cgccgcagcg ccgcctcggc ccgcgcatgc
     3421 ttttcgctca gggtgacgta ggcctgccgc agccagatcg cacccagggc cacgaccgag
     3481 agcagcagcg ccgcagccag cgacaccgcc atgcgcgtga acatgcgccg ttccgcggcc
     3541 cgcacctcgt cgagccgcag cccaaggcgg agcgctccga tctcgccctc cccgaggtcg
     3601 agcgccgagg acagctcgag caccgggcgg ccagccgcct cgcgctcccg ttcttcgacc
     3661 ccgggcgcga ggcccgcgcc cgccacggca ggctccggca aatcgcccgg cagctcccca
     3721 tgcccgtggc gcaggccgtc gcggtccacg acgatgtagg cgagctgcgg ggtggtggcg
     3781 gcgatcgact cgatcaaccg gtcgagcgac acctggcgga gaagcgactc gatctcgctg
     3841 gcgtcggcgt tgacgagaat tgcgccgccg ttctcccggc gcaccgcagc agcgacgcgc
     3901 gatccagcgc gacggccggc ggggcgaagc tcgccgacca cctcccgctg acggccggag
     3961 aggatcggct cgatgaaggc cgcgcccggg cccatccccg tgccgccccc cggagccgtg
     4021 ccgccgggcg ccatgctcgt gccgggcgcg ggcggtacct cctgccgctc gccggctctg
     4081 ggcgcggaac gccggcccgc cggcgcacgc cgccagccag gcgacccggc gcggcccgcc
     4141 acccctcctc cttcacgcgc gccgtcgggg ccgaacaccg ccacgcggaa cagcctgttt
     4201 cgctcggccg tctctgcgag aaagtcgccc gtcaagcttc ccttccgatc cagctcggcc
     4261 agcaggcgcg cgttgtccag cagccgctcg atcacctggg tcgaagcgac ggcgccggct
     4321 gcctggttcg accgcgcggc cgccgccacc gtctggcgaa gggcggcgcc ctggccgcgc
     4381 aagagcccca gcagctcgcg gcgggtggcg ctgtagtcga accagccgac gaacaccagg
     4441 cctgcggccg cgagcagcgt ggccgcgacg atgcgtgccg tgctgagggt gggcttcatc
     4501 ccggttgaat cccgccgccg atgggcggcc acaagggccg cgcctacggc ttctccaata
     4561 gcttctccat ctcgtccatc acccggttca tctgcttcat cgtcaggtcg agcggctcgc
     4621 gcgtcgtcat gtccacgccg gcgcgccgca ggagggcgat gggatagtcg gagccccccg
     4681 ccgagatcag ctcgaggtac ttgcggatgg tctcctggtc gcctgcgaga gcccgctcgg
     4741 ccaacgcggc cgaggcggtg aaggaggtgg cgtactggta gacgtagaag gcccggtaga
     4801 agtgggggat gaaggcccac tcgtgcgcca cgtagtcgtc caccacggtg acgcccgcgc
     4861 cgtggccata gtaccgcttg
//
LOCUS       DAOPQX010000285         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19036_length_4854_cov_4.637841, whole genome shotgun sequence.
ACCESSION   DAOPQX010000285 DAOPQX010000000
VERSION     DAOPQX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19036_length_4854_cov_4.637841"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="PKK95_13385"
     CDS             <1..1861
                     /locus_tag="PKK95_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOC19259.1"
                     /translation="FAALLELIVQAALSRFARPSLVGLLRSPHLAIEVDGRVPGAAAV
                     SRLERALLDAGYGGNPARLAVLAGEWTDELAHAARAAAAIATELSPLLVRAPCSAHIA
                     TIRAFLLAHERPVDAADAMGQRHLRARAAAMSILDGLAEASRRFDDPVVPLEEVAAQL
                     RRWMEGQTFAPSEPAAGSDGVHLVDARAARFGAFDAVHLAGLVAGEWPEPPRREILYP
                     QFLLTQLGWPGASDYAAAARAEFEDLLDLSPGATSVSTFLLENDTIVEPSPLLDAVEP
                     PTDVPATEAVVYPEERPPDEGAAGMEGRGVRRDATSGAGETWRSIRCGRTAPSDPRYH
                     GQAGETRVGTLSISAIDRYLDCPFKYFAASVLRLEEEPEADPALAPKARGIFVHELFR
                     AFFAAWQREGGGAITAVEMPRARALFAQVVEGQLEHLPPGQRAAERLRLLGSVASAGL
                     GEVVFEAEALRPVPVVERLLEYPFDGAFVLEAGGEARRVRLRGKIDRVDVLSDRTLRV
                     IDYKTGRAPDSKRSIQLPAYLLCAAQQVSRERGASFTAGEALYVAFGEPEPVQWVVGP
                     DLAPTAADEAVSRLVGAVDGIAAGRFPPQPATRRLCVTCGFSAVCRKDYVDAE"
     gene            1851..4676
                     /locus_tag="PKK95_13390"
     CDS             1851..4676
                     /locus_tag="PKK95_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOC19260.1"
                     /translation="MPSERRRAARPGVDAGAFPRLPFDDAPGTPDPLAARDEEARAFA
                     VDPSRNVVLEASAGTGKTRVLTTRYVNLLRAGVEPRNILAITFTRKAAAEMRERILAQ
                     LKELARLSPADAARWRDLRDRQGDIAISTIDAFCLSLLREFPLEAGLDPGFQVVDEAE
                     APRLVEEALDAALRAARRLTAVDPDVALLHARLGERRLREALAALVGRRLVAGRLMRR
                     FLGGAGAPRSEAGAAETSLRDALERMRALLSRGEEGIEGFLADGPIRHPAFALLEADV
                     RALDSGRVEAPRAVLDRLARHFLTRAGRPRSKPAPSFRRSDFGSPEAWRRHGQVVASL
                     APYVSDVLGDLDRSVNRAMVRGLSRLFRVARLRYLRALNASAAVDFTEGLVRALALLR
                     RMDEFSQSRYRLEARYHHVLVDEFQDTSRAQWALVARLVEAWGEGAGLAHDAPLQPSI
                     FVVGDRKQSIYAFRDADVRLLRHARRYVGALRPGGGVRRSIARSFRSDAAVLAFVNDL
                     CAEFQVAPGRPDAFRYRASDRFPVAPGPGGGEERLGLVAEPTVSAASQAVAAEIERLL
                     REETVRDRRTGTARAATPGDIAVLFRSRESHREFEAALEARGIPGYVYKGLGFFDADE
                     VKDVLALVRFLADPASDLRAAALLRSRFIRLSDPGLARLAPSLAAALTAPEEAAVEGL
                     SEEDARVLRQARASLAAWLSRVDRVPPAELLDAILAESAFDAELAGPRQAQARENLKK
                     LRALARRVQNRGYATWARLTGHLDRLSAGDESNAIVDAADAVNLMTVHAAKGLEFAFV
                     FLVNIGRGTGGGGEPVLLLDDPRSHRALVSVQGGLKEAEEAARERDREETKRLLYVAL
                     TRARERLYLAAVVRDGRLQPGRGSLAEVLPASFLEVMAAAGAGSSRVTRTEWISREGG
                     RHAFRVCRAPAPDGNGVPAVSGVTAGP"
BASE COUNT          577 a         1759 c         1832 g          686 t
ORIGIN      
        1 gtttgccgcg ctgctcgagc tgatcgtcca ggccgcgttg tcgcggttcg cccgcccgtc
       61 gctcgtcggc ctcttgcgct cgccccacct ggcgatcgag gtggacgggc gcgtccccgg
      121 cgcggccgcg gtcagccgcc tcgagcgggc gctcctcgac gccggctacg ggggaaaccc
      181 ggccaggctc gccgtgctcg ccggcgagtg gaccgacgag ctcgcccacg cggcacgggc
      241 agcggctgca attgcgacgg agctctcgcc gctcctcgtg cgcgctccct gctcggcgca
      301 catcgcgacc attcgcgcct tcctcctcgc gcacgagcgc cccgttgacg ccgccgacgc
      361 catgggccag cggcaccttc gcgcccgtgc ggcggcgatg tcgatcctgg acggtctcgc
      421 cgaggcgtcg cggcggttcg acgacccggt ggttccgctc gaagaggtcg cggcgcagct
      481 gcggcggtgg atggagggac agacattcgc cccgagcgaa ccggccgccg ggtcggacgg
      541 cgttcacctg gtggacgccc gcgccgcacg attcggcgcc ttcgacgccg tccacctcgc
      601 aggcctcgtg gcgggagagt ggcccgagcc gccgcgccgc gagatcctct acccgcagtt
      661 cctgttgacc cagctgggtt ggccgggggc ctcggattac gcggcggctg cgcgtgccga
      721 gttcgaggac ctgctggatc tctccccggg cgcgacctcg gtgtccacct tcctcctcga
      781 gaacgacacc atcgtcgagc cgtcgcccct gctcgacgcg gtggagccgc caaccgacgt
      841 ccctgcgacc gaggccgtcg tttaccccga ggagcggccc cccgacgagg gcgcagccgg
      901 catggagggc cgtggcgttc ggcgcgacgc cacctccggc gccggcgaga cgtggcggtc
      961 gatcaggtgt ggtcgaaccg cgccgagcga ccctcgctat cacggccagg ccggcgagac
     1021 gcgggtcggc acgctctcca tcagtgccat cgaccggtac ctcgactgcc cgttcaagta
     1081 cttcgccgcc agcgtgctgc ggctggaaga ggagccggag gccgacccgg cgctcgcccc
     1141 gaaggcgagg gggatcttcg ttcacgagct gttccgtgcc ttcttcgcgg cgtggcagcg
     1201 ggagggcggg ggcgcgatca ccgccgtcga aatgccccgc gcacgcgcgc tgttcgccca
     1261 ggttgtggaa gggcagctgg agcatctgcc gccgggccag cgcgcggccg agcggctgcg
     1321 cctgctcggt tcggtggcct ctgccggcct tggcgaggtc gtcttcgagg ccgaggccct
     1381 gcggcccgtc ccggtggtcg agcggctgct cgagtacccg ttcgacggcg cgttcgtgct
     1441 cgaggccggt ggagaggccc ggcgagtgcg gctgcgcggg aagatcgacc gggtggacgt
     1501 gctgtccgac cggacgctgc gcgtgatcga ctacaagacc gggcgagccc cggactcgaa
     1561 acgctcaatc cagctgcccg cctacctgct ctgcgccgcg cagcaggtct cgcgcgagcg
     1621 cggcgccagt ttcacggctg gcgaggccct ctacgtcgcg ttcggcgagc ccgagccggt
     1681 gcagtgggtc gtggggcccg atctcgctcc cacggccgcg gacgaggccg tctcgcggct
     1741 cgtcggggcg gtggacggca tcgcggcggg ccgtttcccg ccgcagccgg ccacgcggcg
     1801 gctgtgcgtc acctgcgggt tcagcgccgt ctgccggaag gactacgtcg atgccgagtg
     1861 aacgccgccg cgcggcgcgc ccgggcgtgg acgccggcgc ctttccgagg ctgcccttcg
     1921 acgatgcccc aggcacgccc gacccgctgg cagctcgtga cgaggaggcc cgcgccttcg
     1981 cggtggaccc gtcgcgcaat gtggtgctcg aggcctcggc cggcaccggg aagacgcgcg
     2041 tcctgaccac gcgctacgtg aacctgctgc gcgccggcgt cgagccgcgc aacatcctgg
     2101 ccatcacctt cacgcgcaag gcagccgccg agatgcggga gcggatcctg gcgcagctca
     2161 aggagctggc gaggctgtcg cccgccgacg ccgcccgctg gcgcgacctg cgcgaccgcc
     2221 agggcgacat cgccatcagc acaatcgacg ccttctgcct ctcgctgctt cgcgagttcc
     2281 cgctcgaggc ggggctcgac cccgggttcc aggtggtgga cgaggccgag gcgcctcgcc
     2341 tggtggagga ggcgctcgac gcggcactgc gggcggcgcg caggctgacc gcggtcgatc
     2401 ccgacgtcgc cctgctgcac gcccggctcg gagagcggcg gctgcgcgag gcgctggcgg
     2461 cgctggtggg gcggcgcctg gtggcggggc ggttgatgcg ccggttcctt ggcggggcgg
     2521 gcgcgcctcg gtcggaggca ggcgccgcgg agacctcgct cagggacgcg ctcgagcgga
     2581 tgcgcgcgct gctgtcgcgc ggcgaagaag ggatcgaggg cttcctggcc gacggcccga
     2641 ttcgccaccc ggcgttcgcg ctgctcgagg ccgacgtgcg ggcgctcgat tcgggccgcg
     2701 tcgaggcgcc gagggcggtg ctcgaccgcc tcgcccgcca cttcctgacc cgcgccggga
     2761 ggccgaggtc gaagccggcg ccgtcgtttc ggcgcagcga cttcgggtcg cccgaggcct
     2821 ggagacgcca tggccaggtg gtggcctcgc tcgcgccgta cgtctccgac gtgctgggtg
     2881 acctcgaccg ctcggtgaac cgcgcgatgg tgcgcggcct gtcgcgcctt ttccgcgtgg
     2941 cccgcctgcg atacttgcgg gccctcaacg ccagcgcggc cgtggacttc accgaagggc
     3001 tggtgcgcgc cctcgccctg ctgcgccgga tggacgagtt ctcgcagagc cgctaccggc
     3061 tcgaggcgcg gtatcaccac gtcctggtgg acgagttcca ggacaccagc cgggcgcagt
     3121 gggcgctggt ggcgcggctc gtcgaggcct ggggcgaggg agccggcctc gcgcacgacg
     3181 ccccgctcca gccctcgatc ttcgtcgtcg gcgaccgcaa gcagtcgatc tacgccttcc
     3241 gcgacgccga cgtgcggctg ttgcggcacg ccaggcgtta cgtcggtgcg ctccggccgg
     3301 gcggcggcgt tcgccgctcg atcgcgcgaa gcttcaggtc ggacgcggcc gtgctcgcct
     3361 tcgtgaacga cctgtgcgcg gagttccagg tggcacccgg gcgtcccgac gcgttccggt
     3421 accgggcctc ggaccgcttc ccggttgccc ccggcccggg cggcggcgag gagcggctcg
     3481 gcctggtggc cgaaccgacc gtgagcgcgg cctcgcaggc ggtggcggcg gagatcgaac
     3541 gcctgctgcg cgaagagacc gtccgcgacc ggcgcaccgg gacggcgagg gcggccaccc
     3601 ccggcgacat cgcggttctc ttccgctcgc gcgagagcca ccgcgagttc gaggcggcgc
     3661 tcgaggcgcg cggcattcca ggctacgtct acaagggcct cgggttcttc gatgccgacg
     3721 aggtgaagga cgtgctggcg ctcgtccggt tccttgccga cccggcatcc gacctgcgcg
     3781 cggcggcgct gctgcgctcg cggttcatcc gcctgtccga cccagggctg gcgcgcctcg
     3841 cgccttcgct ggcggcggcc ctgaccgcgc cggaggaggc cgcggtggag gggctgagcg
     3901 aggaggacgc ccgcgtcctg cgccaggcgc gcgcctccct cgccgcctgg ctgtcccgcg
     3961 tggatcgggt gcccccggcc gaactgctcg atgccatcct ggccgagtcg gctttcgacg
     4021 ccgagctcgc cgggccccgg caggcacagg cgcgcgagaa cctgaagaag ctgcgcgcgc
     4081 tcgcgcgccg cgtgcagaac cgcggctacg ccacctgggc gcgcctgacc gggcatctcg
     4141 accgcctgtc ggccggcgac gagtcgaacg cgatcgtcga cgcggccgac gccgtgaacc
     4201 tgatgaccgt gcacgcggcc aaggggctcg agttcgcctt cgtgttcctg gtcaacatcg
     4261 ggcgcggcac gggcgggggc ggcgagcccg tgctgctgct cgacgacccg cgctcgcacc
     4321 gggcgctggt gtcggtgcag ggggggctga aggaggccga ggaggcggcg cgcgaacgcg
     4381 atcgtgagga gaccaagcgg ctgctctacg tcgcgctgac ccgcgcgagg gagcggctct
     4441 acctggccgc cgtggtgcgc gacgggcggc tgcagccggg ccgcggcagc ctggcggagg
     4501 tgttgcctgc cagcttcctg gaggtgatgg cggcggcggg ggcgggcagc tcccgcgtca
     4561 cgcgcaccga gtggatctcg cgcgagggag ggaggcacgc gttcagggtg tgccgcgcgc
     4621 cggcacccga cgggaacggc gtgccggctg tgagcggcgt cacagcaggg ccgtgatcga
     4681 agtcactcgc cttcacggcc cggttcctgt atgcttcgcc gaatctccac gcgctttgaa
     4741 accgagttgg ggtggacggc gtctaagaga gtgaacgggg accaccgatg accgccgaca
     4801 gcacgacccg gccgccgacg cgcacctcct gggacgcgca cctgcagctc gaga
//
LOCUS       DAOPQX010000286         4828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19200_length_4828_cov_5.536350, whole genome shotgun sequence.
ACCESSION   DAOPQX010000286 DAOPQX010000000
VERSION     DAOPQX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19200_length_4828_cov_5.536350"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKK95_13395"
     CDS             complement(<1..185)
                     /locus_tag="PKK95_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19261.1"
                     /translation="MTHTSLFRARTVRAACLAAAVSLAVAGVPSVALIRAQQAPPVQP
                     LPPPQQPPEQVPPQQQGP"
     gene            complement(352..1743)
                     /gene="rlmD"
                     /locus_tag="PKK95_13400"
     CDS             complement(352..1743)
                     /gene="rlmD"
                     /locus_tag="PKK95_13400"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOC19262.1"
                     /translation="MPRATMVTVEIERLSLEGDGVATHAGRELRVPQVLPGERVSVRL
                     QTGKDGLRGEVVEVLLASPHRVVPRCRHFGPCGGCAWQHVEYGEQLRLKQQLLQEALD
                     EALGTRAPRVSPAVRSPASSPGATPWEYRNKASFTFAPAEGPGPLVMGHYRRGSRRVL
                     PVVECPVHAGPANRLAFALRDALRAARIPGATPDAAAGIARHVVLRVAERTGEWLATL
                     VVTENVKQLRRATARFLAAASREDRPGGFHLSVHDRDDPFLFGRETRRLDGLAEIREQ
                     VAGATFLLQPDAFFQTNVRVAGEMVARVMAALPAPGFPRVLDLYSGAGLFALPLAAAG
                     ATVTAVEENRGAVASAEAARRLNRLPAARLALVAGRVEETLARLTPGPHLRYDAVVLD
                     PPRPGCPPVVLDWIAGRLHPRRIAYVSCNPSALARDLGAALAGGFRAVEVIPFDMFPH
                     TAHIESIAILDRS"
     gene            1773..4478
                     /locus_tag="PKK95_13405"
     CDS             1773..4478
                     /locus_tag="PKK95_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOC19263.1"
                     /translation="MPEYRTVCPRNCYCTCGMLVTVEGGRVVRIEGDPANPATGGHVC
                     LKGISYARRLTTPDRLLSPLKRTASGAFEPVGWDETLADIAARLDAIRREDSPEAVLY
                     YDASGSHGALGALALAFWRQFGGCTRAFGDLCWPAGLEATRLTYGANRHNHPRLTAES
                     RFILVWGHNPAETNVHQMRLILEAQERGARVAVVDPRGTDTSDAADLHLQPRPGTDAA
                     LALGLARVIAGADLHDEAFLAAHASGFERYLARLEEYPLARVADITGVPAASIETLAL
                     EYAQSKPALLIAGFGLQRNRHAGQTMRAVALLPALTGNVGVPGGGFQYANLASHCLLE
                     PRLPPGSDGRAAIPVSRLGPGLLELDAPRVRAAWIEKGNPASQNPRSDLVRQALANMD
                     LVVVVDQFLTDTARLAHYVLPAKTMFEEEDLCTAYWHPYLQRRAKVFDPPAGVKTETE
                     IWRLLAGRLGFDTRYFPSTPDEARAVLRQMLGPSHAHLLEALENGPVDPSGDGDVAFA
                     DRCFPTPSGKVEFCSDEAASLWGVDPVPGYEPLGEGHESRGAGRFPLQLLSCKTRDRI
                     HSQFGNLDWVRDVERPHRLDIHPADAGPRGLLDDDLAVVWNGRGRIELRVRLDEGLRP
                     GVVHVLEGRCHEGDPDVNVLTDAGVTDMNYGATFYECLVEVEKSTKSGSREVAKSKST
                     KSAKSTKSAKARSESREVGKSESPAFVLDLGRCVGCGACVLACRLENGWRPETPWRRV
                     LPLNLRRRPGGPTYFLSVACHHCERPACAAACPSSAYEKRPDGVVVHHEERCIGCRYC
                     EMACPFGAPRYSAALGVMTKCHLCAPRLDRGELPACVAACPTEALRPSHEGLTLGRDQ
                     QLAAVPGFADRDGCRPRLRFLSPRGSRRMSLMQALQHALARGGER"
     gene            4475..>4828
                     /locus_tag="PKK95_13410"
     CDS             4475..>4828
                     /locus_tag="PKK95_13410"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HOC19264.1"
                     /translation="MSLVKRAARGAADDWPLVVFTSMAIIGAGLVLTPIAALAGGSGA
                     AAAVRLAGAGAALIAAALAVSLAHLGRPLRAARAAANLRQSRLSAEVLFALATLAAAL
                     ISFAWPALLPGASMVA"
BASE COUNT          671 a         1780 c         1754 g          623 t
ORIGIN      
        1 ggcccctgct gctggggcgg aacctgctcg ggcggctgct gcgggggcgg cagtggctgc
       61 acgggcggcg cctgctgcgc acgaatcaag gcgacggagg gcacccctgc caccgcgaga
      121 gagaccgcgg cggcgaggca ggcggcgcgg acggtgcgcg cccggaacag gctggtgtgg
      181 gtcaagggtg gatcccggga tggaggcgct cagcgccgac cagcatacac caggtcgcag
      241 cggcgctcag ggatcaggga tcagggatcg tggatgccga cggcggcggc gcccgccggg
      301 ccgagcagca cgccgatgac gctgtccgcc cgcgggggca gaggcgccgc gtcaactccg
      361 gtcgaggatc gcgatcgact cgatgtgcgc ggtgtgcggg aacatgtcga acggaatcac
      421 ctcgaccgcg cgaaagccgc cggcgagggc ggcacccagg tcgcgcgcca gcgctgacgg
      481 gttgcacgac acgtacgcga ttcgccgggg gtggaggcgg ccggcgatcc agtcgagcac
      541 caccggcggg cagcccgggc gcgggggatc gagcaccacc gcgtcgtacc gcaggtgagg
      601 gccgggcgtc agccgcgcca gggtctcctc gacgcggccg gcgacgagcg ccagccgcgc
      661 ggccggcagg cggttgaggc gccgcgccgc ttccgcgctt gcgaccgcgc cccggttctc
      721 ttcaactgcg gtcaccgtcg cgccagccgc ggcgagcggc agcgcgaaca gccccgcgcc
      781 ggagtagaga tcgagcacgc gcggaaagcc tggcgccgga agagccgcca tcacgcgcgc
      841 caccatctcg ccggccaccc gcacgttggt ctggaagaag gcgtcgggct ggaggaggaa
      901 ggtggcgccc gccacctgct cgcggatctc agccaggccg tccaggcgac gcgtctcgcg
      961 gccgaagaga aacgggtcgt cgcggtcgtg cacgctcaag tggaagccgc cgggccggtc
     1021 ctcccgtgag gcggccgcga ggaagcgcgc ggtcgcccgg cgcagttgct tcacgttctc
     1081 cgtcacgacc agcgtcgcca gccactcgcc ggtgcgctcg gccacccgca gcaccacgtg
     1141 tcgcgcgatg ccggcggcgg catcgggggt ggcaccgggg atccgcgccg cgcgcaacgc
     1201 gtcgcgcagg gcgaaggcga ggcggttggc gggaccggcg tgaaccgggc actcgaccac
     1261 gggcagcacc cgccgcgacc cccggcgata gtggcccatc acgagcggtc caggcccctc
     1321 ggccggcgcg aaggtgaagc tggccttgtt ccggtactcc cagggggtgg cccccggcga
     1381 cgaggcgggc gacctcaccg cgggcgagac gcgcggcgcc cgtgtgccga gcgcctcgtc
     1441 gagcgcctcc tggagcagct gctgcttcaa gcgcagctgc tcgccgtact cgacgtgctg
     1501 ccacgcgcag ccgccgcagg ggccgaagtg gcggcaccgg ggcacgacgc gatgcggcga
     1561 ggcgagaagg acctcgacca cctcgcctcg aaggccgtcc ttgcccgtct gcaggcggac
     1621 cgacacgcgc tcgccgggaa gcacctgcgg cacgcgcaac tcgcgcccgg cgtgggtggc
     1681 gacgccgtca ccctcgagcg acaggcgctc gatctcgacg gtaaccatcg tggcgcgagg
     1741 cacccggcga ttctacggta cactgtcgcg ccgtgccgga ataccgcacc gtctgcccgc
     1801 gcaactgcta ctgcacgtgc ggaatgctcg tcaccgtcga gggcggccgt gtcgttcgca
     1861 tcgagggcga cccggccaac cccgcgaccg gcgggcacgt gtgcctgaag gggatcagct
     1921 acgcgcgccg gctgaccacg cccgaccggc tgctatcgcc gctgaagcgt accgccagcg
     1981 gggcattcga gccggtgggc tgggacgaaa cgcttgcgga catcgccgcg aggctcgacg
     2041 cgatccggcg cgaggacagc cccgaggcgg tgctctacta cgacgcctcg ggcagccacg
     2101 gcgcgctcgg cgcgctggcg ctcgccttct ggcgccagtt cgggggctgc acgcgcgcct
     2161 tcggcgacct ctgctggccc gcgggcctcg aggccacacg cctcacctac ggcgcgaacc
     2221 gccacaacca tccgcgcctg acggcagaga gccgcttcat cctggtgtgg ggacacaacc
     2281 ccgccgagac caacgtccac cagatgaggc tcatcctgga ggcgcaggag cgcggcgccc
     2341 gcgtggcggt tgtcgatccg cgcggcaccg acacctccga cgcggccgac ctgcacctcc
     2401 agccgcgccc cgggaccgac gccgccctcg ccctgggcct cgcgcgcgtc atcgccggag
     2461 cggacctgca cgacgaggcg ttcctcgccg cgcacgcctc gggcttcgag cgctacctcg
     2521 cccggctcga ggagtacccg ctcgcccggg tggccgacat caccggcgtg ccggcggcat
     2581 cgatcgaaac gctcgcgctc gagtacgcgc aatcgaagcc cgcgctgctg attgccggct
     2641 tcggcctgca gcgcaatcgt cacgcgggcc agacgatgag ggcggtcgcg ctgctgccgg
     2701 cgctcaccgg aaacgtcggc gtccccggcg gcgggttcca gtacgccaac ctggccagcc
     2761 actgcctcct cgagccgcgg ctgccgccgg gatcggacgg ccgcgccgcg attccggtct
     2821 ctcgcctcgg cccggggctc ctcgagctcg atgcgccccg cgtgcgggcc gcctggatcg
     2881 agaagggcaa ccccgccagc cagaaccccc gctccgacct ggtgcgccag gccctcgcca
     2941 acatggacct ggtggtcgtc gtcgaccagt tcctcaccga cacggcgcgc ctcgcacact
     3001 acgtgctgcc ggccaagacg atgttcgagg aggaggacct ctgcacggcg tactggcacc
     3061 cctacctcca gcgccgcgcg aaggtgttcg acccgccggc cggcgtcaag accgagaccg
     3121 agatttggcg gctgctcgcc gggcgcctcg ggttcgacac tcgttacttc ccgagcacgc
     3181 cggacgaggc gcgcgccgtg ctccgtcaga tgctcgggcc ctcccacgcg cacctgctcg
     3241 aggcgctcga gaacggcccg gtcgatccct ccggcgatgg ggacgtcgcc ttcgccgatc
     3301 gctgcttccc aacgccgtct gggaaggtgg agttctgctc cgacgaggcg gcctcgctgt
     3361 ggggcgtgga cccggtgccc ggctacgagc cgctcggcga gggacacgag tcgcgcggcg
     3421 ccgggcgctt cccgctgcag ttgctgtcgt gcaagacgcg cgatcgcatc cactcgcagt
     3481 tcggcaacct ggattgggtg cgcgacgtcg agcggccgca ccgcctcgac atccacccgg
     3541 ccgacgccgg gcccagggga ctcctcgacg acgacctcgc cgtggtctgg aacggccgcg
     3601 gccggatcga actgcgcgtg cggctcgacg aggggctgcg ccccggtgtc gtccacgtgc
     3661 tggaggggcg gtgccacgaa ggcgatcccg acgtcaacgt gctgaccgac gcgggcgtca
     3721 ccgacatgaa ctatggggcg acgttctacg agtgcctcgt agaggtcgag aagtccacga
     3781 agtcgggaag tcgcgaagtc gcgaagtcga agtccacgaa gtccgcgaag tccacgaagt
     3841 ccgcgaaggc gaggtcggaa agtcgtgaag tcggaaagtc ggagtctccc gccttcgtcc
     3901 tcgacctcgg gcggtgcgtg gggtgcggcg cctgcgtgct ggcgtgccgc ctcgagaacg
     3961 ggtggaggcc cgagacgcca tggcgccggg tgctgccgct caacctgcgg cgccggcccg
     4021 gcggccccac ctacttcctg tcggtggcgt gccatcactg cgaacgcccg gcgtgcgcgg
     4081 ccgcctgccc ctcgtccgcg tacgagaagc ggccggacgg cgtggtcgtg caccatgaag
     4141 agcggtgcat cggctgccgc tactgcgaga tggcctgccc gttcggcgcc ccgcgatact
     4201 cggccgcgct tggcgtgatg accaagtgcc acctgtgcgc accgcggctc gaccgcggtg
     4261 aactgccggc gtgcgtcgcc gcctgcccaa ccgaagcctt gcgcccgagc cacgagggcc
     4321 tgaccttggg acgagaccag cagctggccg ccgtgcccgg gttcgcggat cgcgacggct
     4381 gccggccgcg cctccgtttc ctgtcgccga gggggagccg ccggatgtcg ctgatgcagg
     4441 cgctgcagca cgcgctcgcg cggggaggtg agcgatgagc ctcgtgaagc gtgccgcccg
     4501 tggcgcggcc gacgactggc cgctcgtcgt cttcacctcg atggccatca tcggcgccgg
     4561 cctcgtcctc accccgatcg ccgcgcttgc cgggggaagc ggagcagccg ccgcggttcg
     4621 cctcgccggg gccggggctg cgctgattgc ggcggccctg gccgtatcgc tggcgcacct
     4681 tgggaggccg ctccgggccg cgcgcgccgc tgccaatctc cggcaaagcc ggctgagcgc
     4741 ggaagtcctc ttcgcgctgg ccaccctggc tgccgccctc atctcgttcg cctggcccgc
     4801 cctgctgccc ggcgcgagca tggtggcc
//
LOCUS       DAOPQX010000287         4817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19266_length_4817_cov_4.621798, whole genome shotgun sequence.
ACCESSION   DAOPQX010000287 DAOPQX010000000
VERSION     DAOPQX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19266_length_4817_cov_4.621798"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PKK95_13415"
     CDS             complement(<1..921)
                     /locus_tag="PKK95_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC19265.1"
                     /translation="MQDSSAAAGGPNPDAQGTDLGDVADTATDATSTPPRPAPIDIRS
                     AALAVIAALLSLAALRLAAPVLIPLAFAILASYALNPVVTTLVGWRIQRPLAALLVLS
                     VMTAGAGYLVYSMTDDVAAVAAKLPKAADRMRQTLRDLRRSGGATSPVQQVQQAAETL
                     EAAAAEAAGESPVAARSRTRAANGPVLNLNAWLMTGSLSLVAVAAEAVVVLFLALYLL
                     AAGDLFKRKLVRVVGTTLSEKKITVQILDEIDRQIATFLLVRLVITVLVAVFTWLPLW
                     AIGLEQAALWGVAAGVLNIIPYLGPVIVAAV"
     gene            1454..3193
                     /locus_tag="PKK95_13420"
     CDS             1454..3193
                     /locus_tag="PKK95_13420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOC19266.1"
                     /translation="MRTQPGSRPGMRAALEARGYHVRGEHSALDAIVADVPAGELEAL
                     SLMPGVQGISSDAIVRPAGLDLAAATSAGTSPENFLRSTLGLDRSSPVGLGVGVAVID
                     SGVAPSRDLLPQITAFFTVSESGLRRSAPYDDFGHGTHVAGLIAGSGAGAARDYQGVA
                     PGVRLAVFKVLDGSGQGRTSDVVRAIEFIVANRRMLGVDVINLSLGHPVFEPADTDPL
                     VQAVQQATRAGLVVVAAAGNFGTNPVTGEPGYAGITSPGNAPSAITVGAASTKGTIGR
                     RDDGVAPFSSRGPTWYDAYAKPDIVAPGEKLTSVGVPGTPLYDNYPTLQVDGTKGGTY
                     LRLSGTSMATAVATGVVALAIEANRRQNGGPPVAASRRQLSANAVKGILQYSAFSLID
                     ESGEAYDRLTQGGGGLNAAGAIDLASRLDLQAPVGEAWIEPVPDPVTVIAGEELAWSQ
                     NIVWGVWLVPGSVFETNNECWGTNIVWGVSDGQNIVWGVSDTQLAGANIVWGVNIVWG
                     VNVVWGVNIVWGVNIVWGVNLVGTSTASGVSWGFPGGPDTTAWSRLDASNIVWGVADN
                     IVWGVAENIVWGV"
     gene            3234..3893
                     /locus_tag="PKK95_13425"
     CDS             3234..3893
                     /locus_tag="PKK95_13425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOC19267.1"
                     /translation="MDRMPHPAAVEVPAVAPIADATRTAPACWRSTLPELGDGRVTLR
                     PMVARDATSLFAMLTTEEVTRFISPPPPDLGGFERFIEWSNAEWKAGRFAAFAIVPTG
                     YDVSVGLVQVRQLDPGFSNAEWGIALGSPFWGTGVFPSAARLFLDFVFGEIGVHRLEG
                     RAAVQNGRANGALRKIGAVQEGLLRRALFCNGRHFDQLLWSILAEDWREASATLRPMV
                     H"
     gene            3944..>4817
                     /gene="queA"
                     /locus_tag="PKK95_13430"
     CDS             3944..>4817
                     /gene="queA"
                     /locus_tag="PKK95_13430"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOC19268.1"
                     /translation="MDVALFDFELPPGQIAQRPAERRDVSRLLVLERASGAIRAHGRV
                     ADLPELLSPGDLLVTNDTRVFPARLLGRRDPSGGAVECLLLRRLDRERWEALVHPGQK
                     LKPGKLMVFEGPGMVLHGEILERHFHGRRTIRLWAEGGLDAEPVIDAIGHIPLPPYIH
                     RSDEREDRERYQTVFARARGSVAAPTAGLHFTSELLDRLEARGVERAEVTLHVGYGTF
                     KPVHAEHVEEHRVDEEVYDIPEEAAARIERARREGRRVIAVGTTTTRALEAAAIEGRG
                     RVTAGRAATGLFIYP"
BASE COUNT          704 a         1618 c         1750 g          745 t
ORIGIN      
        1 gacggccgcc acgatcaccg gcccgaggta ggggatgatg ttgaggacgc cggccgccac
       61 cccccacagc gccgcctgct cgaggccaat cgcccaaaga ggcagccacg tgaagacggc
      121 caccagcacc gtgatcacga ggcggacgag caggaaggtc gcgatctgcc ggtcgatctc
      181 gtcgaggatc tggaccgtga tcttcttctc ggaaagggtc gtgcccacca cgcgcaccag
      241 cttccgcttg aacaggtctc ctgcggcgag gaggtacagc gcgaggaaga ggaccaccac
      301 ggcctcggcg gcgacggcca ccaggctgag cgagccggtc atgagccacg cgttcaggtt
      361 cagaaccggg ccgttggccg ccctcgtgcg gctcctcgcg gcgaccggtg actcgccggc
      421 cgcctcggcc gcggccgctt ccagcgtttc tgcggcctgc tgcacctgct gaaccgggct
      481 cgtggcgcct cccgagcggc gcaggtccct gagcgtctgc cgcatcctgt cggcggcctt
      541 tgggagcttt gccgcgaccg ctgccacgtc gtcggtcatg gagtagacga ggtagccggc
      601 tccggccgtc atcaccgaca ggacgagcag cgccgccagc gggcgctgga tgcgccagcc
      661 cacgagcgtg gtcacgacgg gattgagggc atagcttgcc aggatcgcga aggcgagcgg
      721 gatcagcacg ggagccgcca ggcgcagcgc ggcaagcgac aggagcgcgg cgatcacggc
      781 gagcgccgcg ctccggatgt cgatcggcgc gggacgcggc ggcgtggaag tggcgtcggt
      841 cgccgtgtcg gcgacgtcgc ccaggtcggt gccctgggcg tcggggtttg ggccgccggc
      901 ggcggcggaa gagtcctgca tcgttggtga cgtaaccagc tccagcgata gcaaggacac
      961 agccagaagg ggctcacggc tcacggctca cggctcaggg tggcgaaggc gggagcatca
     1021 cgatcgttgg gcgaaggcga gctcatagtg tctgagccct tctggcacag agctctgtgc
     1081 cgttctggga caggccattt cggcgttttt cggcaggaat tgcagaattt gcgatcgacc
     1141 cccctgccaa tctgtgccgt agttgcacac ccgcgtgccc ggacgctcgc gggttaccaa
     1201 tgggcattcg ccataactgt cctgaacata atgacttgca ggatgcgtcc gcacccccgc
     1261 ccgaggtggc tggcagattg ctctagagct ggccgtgagc actactcctt cgtttttctt
     1321 cagaaaatgg cttgcctgcc tggcgctggc ggtcgtggtc gcggcacctg ccgcggccgc
     1381 tccacaacgg cacgggcggc tcgatgcggc tttgcagcgg gcgtcacggg cgggaggtac
     1441 cgaacgcgtc atcgtgcgaa cgcagcccgg gtctcgaccc gggatgcggg cggcgctcga
     1501 ggccaggggc taccacgttc gtggcgagca ctcggccctc gacgccatcg tggccgacgt
     1561 gccggcgggc gaactggaag ccctctcgct gatgcccggg gtccagggga tctcctccga
     1621 cgccatcgtg cgtcctgcgg gcctcgacct cgccgcggcg acttcagccg gcacgtctcc
     1681 cgagaacttc ctgcgatcga cgcttggact cgaccgctcc tcgccggtcg ggctgggcgt
     1741 cggcgtggcc gtgatcgatt ccggcgtggc cccgtcacgc gatctgctgc ctcagatcac
     1801 ggcgttcttc acggtgagcg agtcggggct gcgccgttcg gcgccgtacg acgacttcgg
     1861 ccacggcact cacgtggccg gcctgatcgc cggcagcggg gcaggcgcgg cccgcgacta
     1921 ccagggggtc gcgccaggag tccgccttgc cgtgttcaag gtgctcgacg gctcgggcca
     1981 gggccggacg agcgacgtcg ttcgcgccat tgagttcatc gtggcgaacc ggcggatgct
     2041 cggggtggac gtcatcaacc tgtcgctcgg gcacccggtg ttcgagccgg ccgacaccga
     2101 tcccctcgtg caggcggttc agcaggcgac gcgggcaggg ctcgtggtgg ttgcggcggc
     2161 agggaacttc ggcaccaacc cggtcaccgg cgagcccggg tacgccggca tcacctcgcc
     2221 tggcaacgct ccgtcggcga tcaccgtcgg cgccgcctcc acgaagggga cgatcgggcg
     2281 ccgtgacgac ggggtggctc ccttcagctc caggggaccg acttggtacg acgcctacgc
     2341 caagcccgac atcgtggcgc ccggcgagaa gctgacctcg gtgggtgtgc cgggcacccc
     2401 cctctacgac aactacccga cgctgcaggt ggacggcacc aagggaggca cctatctgcg
     2461 gctcagcggg acgagcatgg ccaccgccgt cgccacgggc gtcgtcgcgc tggccatcga
     2521 agcgaaccgg cgccaaaacg gcggtccgcc ggtcgccgcc tcacgccgcc agctttcggc
     2581 gaacgcggtg aagggcatcc ttcagtacag cgccttctcg ctgatcgacg agagcggcga
     2641 ggcgtacgac cggttgacgc agggcggcgg cggcctgaac gcggccggcg cgatcgacct
     2701 ggcgagccgg ctcgatctgc aggcgccggt gggagaggcc tggatcgagc cggtgccgga
     2761 cccggtgacc gtcatcgccg gggaagagct ggcctggtcg cagaacatcg tgtggggcgt
     2821 gtggctggtg ccgggttcgg tcttcgagac caacaacgaa tgctggggca ccaacattgt
     2881 ctggggcgtc tcggatggcc agaacatcgt gtggggcgtg tcggacaccc agctcgccgg
     2941 cgccaacatc gtctggggcg tcaacatcgt ctggggcgtg aacgtggtct ggggcgtgaa
     3001 catcgtgtgg ggcgtgaaca tcgtgtgggg cgtcaacctc gttgggacct cgaccgccag
     3061 cggcgtctcg tgggggttcc cgggcgggcc ggacaccacc gcctggagcc gcctcgacgc
     3121 gagcaacatc gtgtggggtg tggccgacaa catcgtctgg ggtgtggccg agaacatcgt
     3181 gtggggcgta tgagcgcccc cggacaggcc gtttcagagg ttggaggatg ttaatggaca
     3241 ggatgccgca cccggcggcg gtggaagttc cggcggttgc ccccatcgcg gacgccacgc
     3301 ggacggcgcc ggcgtgctgg cggtcgacgc tccccgaact cggcgacggt cgcgtcacgc
     3361 tgcgccccat ggtggcgcga gacgcgacct cgctgttcgc gatgctcacg accgaggagg
     3421 tcacgcgctt catctcgccg ccgccgcccg acctcggcgg cttcgagcgg ttcatcgagt
     3481 ggtcgaacgc cgagtggaaa gcgggccgat tcgcggcgtt cgcgatcgtg ccgacgggct
     3541 acgacgtttc ggtcggcctg gtccaggttc gccagctcga tcccggtttt tccaatgccg
     3601 agtggggcat cgcgctcggc tcgccgttct gggggacggg ggtcttcccc tcggcggcgc
     3661 ggctgttcct cgacttcgtc ttcggcgaga tcggcgtcca ccgcctcgag ggccgggcgg
     3721 ccgtgcagaa cgggcgggcg aacggggcgc tgcggaagat cggcgccgta caggaggggc
     3781 tgctccgacg cgccctgttc tgcaacggcc ggcatttcga ccagctgctc tggtccatcc
     3841 tcgccgaaga ctggcgcgag gcgagcgcga ccctgaggcc gatggtgcac tgagcccgga
     3901 gccccgcgcg tcgggattgg gtcgctcctg atacactccc tgagtggacg tcgcgctctt
     3961 tgatttcgag ctgccgccag gccagatcgc gcagcggccg gcggagcggc gtgacgtatc
     4021 gcgccttctc gttctcgagc gcgcaagcgg cgccattcgc gcgcacggca gggtcgccga
     4081 cctgccggag ctgctttccc cgggcgatct cctcgtcacc aacgacaccc gtgtctttcc
     4141 agccaggctg ctcggccgcc gggatcccag cggcggggcg gtcgagtgcc tgctcctgcg
     4201 gcgtctcgat cgcgagcgct gggaggccct ggttcacccg ggccagaagc tgaagccggg
     4261 caagctgatg gtgttcgagg gcccggggat ggtgctgcac ggggagatcc tcgagcgcca
     4321 cttccacggc cgtagaacca tccgcctgtg ggccgaaggc ggcctcgacg ccgagccggt
     4381 gatcgacgcg atcggccaca tcccgctgcc gccgtacatc caccgctcgg acgagcgcga
     4441 ggaccgcgag cgataccaga ccgtgttcgc gcgggcacgc gggtcggttg ccgcgccgac
     4501 ggccggactg cacttcactt cggagttgct ggaccggctc gaggcgcgcg gcgtcgagcg
     4561 cgccgaggtg acgttgcacg tcggctacgg aaccttcaag ccggtccacg ccgagcatgt
     4621 tgaagagcac cgggtggacg aggaggtcta cgacatcccc gaggaggccg cggcccgcat
     4681 cgagagggcc cgccgcgagg ggcggcgggt catcgcggtg gggacgacca ccactcgcgc
     4741 gctcgaggcc gcggcgatcg agggacgggg ccgcgtgacc gccgggcgcg ccgccaccgg
     4801 cctgttcatc taccccg
//
LOCUS       DAOPQX010000288         4779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19448_length_4779_cov_3.741321, whole genome shotgun sequence.
ACCESSION   DAOPQX010000288 DAOPQX010000000
VERSION     DAOPQX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19448_length_4779_cov_3.741321"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKK95_13435"
     CDS             complement(<1..1029)
                     /locus_tag="PKK95_13435"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOC19269.1"
                     /translation="MTPAFASWYRRNRRRSRAIFDLLAGEAYFSQPIALRHPIVFYDG
                     HLPAFSFNTLVKKALGGASIDERLERLFARGIDPGEAAAGPRFEWPDRDLVREFVEEA
                     DRRVLDVLEREDLDRPGHPLLDRAEAVFAILEHEAMHQETLLYMWHRLPFERKRKPAG
                     YAPKTEGRVPRQEWIEVAGGRVTMGVDPGEVPFSWDNERPHHAAEVAPFVIERHDVTN
                     EAFLEFVEAGGYQAERWWRPEDWAWIRREGIAHPLFWERHGDGWRWRGMFESVALPHA
                     WPAYVSFAEANAYARWRGSRLPSEAEFQRAAYGTPGGAERPHPWGDASPAEEHGAFDF
                     TGWDPQPAG"
     gene            complement(1382..2290)
                     /locus_tag="PKK95_13440"
     CDS             complement(1382..2290)
                     /locus_tag="PKK95_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC19270.1"
                     /translation="MNEHLLSRRALLGTAGAAVLGIVPLKAARASAQAGRKPNSKVAG
                     VQLGVTTYSYRSMPHRVDEVIEYLLFAGVNSIELRLVAEEDLGIPKDKAAQRQWRLSL
                     PMERYDAMRKKFNAAGIDVHIAKFAPSTWSDEEIDYAYEATRRLGAIGITDELGDAAC
                     QRLGKFAEKHKSLAMFHTHGQVAEPGFSFDKYLAYSPANMLNLDAGHYYGATGLHPND
                     VIAKYHDRMRSIHIKDKTGPRHATPNANRPFGQGETPIADILQLLKKEQWPIHVDVEL
                     EYEIPTGSDAAKETKACIDYMRNVLE"
     gene            complement(2305..3852)
                     /locus_tag="PKK95_13445"
     CDS             complement(2305..3852)
                     /locus_tag="PKK95_13445"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC19271.1"
                     /translation="MSDNSISRRHFFFGTLLAGAVPAAGFGSVPSLKALGYQPFSSKL
                     RVAAIGCGGQGAGILDQAARTETVVALCDVDEARAAGSFKKYGGVPRYKDFRVMLDKE
                     GRNIDACTIAVPDFMHATVALWCMERGKHVYVEKPLTRTPWEARLLQDAAVKYKVATQ
                     MGNQGFSHEAHRVAAEIVWTGEIGEVREVHVSTSPGTFPTGLQAPPPEAPIPATLDWP
                     LWLGGAAPRPFSDAYVPYNWRGFLDFGTGQIGNWATHTAGPAHTALQLGVPIAVECVR
                     IEGKGDITIPHRGVVRLDFPARGGMPPVSVYYHESARPGDPEAYHVPGMENETILPPP
                     NNLADKGRRTSERAAPAIGAGVAAGQTGAPTRPRRIMSAGGPDVKVYTQPGGRDDTPR
                     PGVLTGNGSVFIGSKGILATRDRGEGVWLLPASRWQEYLLPPPLLTRSPGHMLDWIRA
                     CKGGDRSCSDFAITASFAEWLALVPIAWRVGGKLEWDSANVRFTNSEEANRSVKPAFQ
                     PGWELKL"
     gene            complement(3881..>4779)
                     /locus_tag="PKK95_13450"
     CDS             complement(3881..>4779)
                     /locus_tag="PKK95_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOC19272.1"
                     /translation="ARMVPLATRWAEGVAEAAGVDYDGETPEGMASQIEAVAGELRDE
                     LRGSDLLRLLPAGGARNALDCALWDLRAKQSGRRAWKAAGLRGMGPVLTDYTIGLGDE
                     AATRRRAREAREYPLLKLKVDGVRHVDQVRIVKEEHPRARIIVDANQAWSRERLEGLL
                     PGLRELGVELIEQPVARGEDACLDGFDSTIPLAADESCTDRCSLARLAGRYRYVNIKL
                     DKCGGLTEALAMAQGARALGLGLMVGNMCGTSLAMAPAFLLAQHCEYVDLDGPLLQVR
                     DREPAMVYRHGRVEPPLPELWG"
BASE COUNT          676 a         1744 c         1606 g          753 t
ORIGIN      
        1 gccggccggc tgcgggtccc agccggtgaa gtcgaaggcg ccgtgctcct ccgctggcga
       61 cgcgtcgccc cacggatgcg ggcgctcggc tccgccgggc gtaccatacg cggcgcgctg
      121 gaactcggcc tcgctcggca gccgcgaccc gcgccacctc gcgtacgcgt tcgcctccgc
      181 gaagctcacg tacgccggcc aggcgtgagg cagcgcgacc gactcgaaca tcccgcgcca
      241 acgccagccg tcgccgtggc gctcccagaa cagcgggtgc gcgatcccct cgcgccggat
      301 ccaggcccag tcctccggac gccaccaccg ctccgcctgg tagccgccgg cctcgacgaa
      361 ctccaggaac gcctcgttcg tcacgtcgtg gcgctcgatg acgaacggcg ccacttcggc
      421 ggcatggtgg gggcgctcgt tgtcccagct gaagggaacc tcgcccgggt cgacccccat
      481 cgtcacgcgc ccccctgcca cctcaatcca ctcctgccgc ggcacgcgtc cttccgtctt
      541 cggcgcgtac ccggcaggct tccgcttccg ttcgaaggga aggcgatgcc acatgtagag
      601 cagcgtctcc tggtgcatcg cctcgtgctc gaggatcgca aacacggctt cggcgcgatc
      661 gagcagcggg tgaccgggcc gatcgaggtc ctcgcgttcc agcacgtcga gcacgcgacg
      721 gtcggcttcc tcgacgaact cgcggacgag gtcacggtcc ggccactcga atcgtggacc
      781 tgccgcggct tcgcccgggt cgatgccgcg cgcgaacagc cgctcgagcc gctcgtcgat
      841 gctcgctccc ccgagagcct tcttcacgag cgtgttgaag ctgaacgcgg gcaggtgccc
      901 gtcgtagaag acgatgggat gacgcagggc gatcggctga ctgaaatacg cctcgccggc
      961 cagcagatcg aagatggccc tcgatcgccg gcggttccgg cgataccacg aggcgaacgc
     1021 aggagtcact gatgaggccg tggctggcac tgatgagtag gacacgcatc cctccagcgc
     1081 ggtccgggct ggcgcacgtg ccgcacccgg gcgtgtaatg gcacgacaca ccgacggtgc
     1141 gcctcttcca gtactcatgc gaaggaatcg gcgcggatga cggtctgagc gatttcagcc
     1201 atccgtgcag gatgagtgcc attgccggca ggagcgccag ggcaggaggt ggcccacctg
     1261 cccttgacct cggccgtccc aatcgcgacc ggcgaggagc gctggagcga gtaccggggg
     1321 cacgaatcac cgcgcgcggg ctacaccgtg gcgcgcgagc ggaagtggac cggggtcgcg
     1381 tctactccag gacgttccgc atgtagtcga tgcaggcctt cgtttccttg gcggcgtccg
     1441 agcccgtcgg gatctcgtac tccagctcca cgtccacgtg gattggccac tgctccttct
     1501 tgagcagctg gaggatgtcg gcgatgggcg tctcgccctg gccgaacggc ctgttggcgt
     1561 tcggggtcgc gtgccgcggc ccggtcttgt ccttgatgtg gatgctccgc atccgatcgt
     1621 ggtacttcgc aatcacgtcg ttcgggtgca ggccggtcgc gccgtagtag tggccggcgt
     1681 ccaggttcag catgttggcg ggtgagtacg cgaggtactt gtcgaacgag aagcccggct
     1741 cggccacctg gccgtgcgtg tggaacatgg ccaggctctt gtgcttctcg gcgaacttcc
     1801 ccaggcgctg gcaggccgcg tcgcccagct cgtcggtgat gccgatcgcc cccagccgcc
     1861 tcgtcgcctc gtacgcgtag tcgatctcct cgtccgacca ggtcgatggc gcgaacttcg
     1921 cgatgtggac gtcgatcccg gcggcgttga acttcttccg catcgcgtcg tagcgctcca
     1981 tcgggagcga cagccgccac tgccgctgcg ccgccttgtc cttcggaatt cccaggtctt
     2041 cctcggccac caggcgcagc tcgatggaat tgacgcccgc gaacagcagg tactcgatga
     2101 cctcgtccac ccggtgcggc atcgagcggt aactgtaggt ggtcacgccc agctggacgc
     2161 ctgccacctt ggagttcggc tttcggcctg cctgtgcgga ggcccgggcg gccttcaacg
     2221 gaacgatgcc gaggacggcg gcgcccgccg tcccaaggag cgcgcgcctg gagagaaggt
     2281 gctcgttcat gatgcctcca gccactacag cttcagctcc caacccggct ggaacgccgg
     2341 cttgacggag cggttggcct cttcgctgtt ggtgaaccgg acgttggcgc tgtcccactc
     2401 cagcttccct ccgacccgcc acgcgatcgg aaccagggca agccactcgg cgaacgacgc
     2461 ggtgatggcg aagtcggagc acgagcggtc gccgcccttg caggcacgga tccagtcgag
     2521 catgtggccc ggcgagcggg tgagcagcgg cggcggcagc aggtactcct gccacctcga
     2581 cgccggcagc agccagacgc cctcgccgcg gtcgcgggtg gcgaggatgc ccttgctgcc
     2641 gatgaacacc gagccgttgc cggtgagcac gccgggccgc ggggtgtcgt cgcgtccgcc
     2701 cggctgcgtg tagaccttca cgtcggggcc gcccgcgctc atgatcctgc gggggcgcgt
     2761 gggagccccc gtctgccctg ccgcgacgcc cgcgccgatt gccggcgccg cccgctcgct
     2821 cgtgcgacgt cccttgtcgg cgaggttgtt cggcggcggc aggatcgtct cgttttccat
     2881 cccgggcaca tggtaggcct cggggtcgcc cggccgtgcc gactcgtggt agtagaccga
     2941 caccggcggc atcccgccgc gcgccgggaa gtcgagccgc accacgccgc ggtgcgggat
     3001 cgtgatgtcg cccttccctt cgatccgcac gcactcgacc gcgattggca cgccgagctg
     3061 cagcgcggtg tgggccggac ccgccgtgtg cgtggcccag ttgccgatct ggcccgtgcc
     3121 gaagtcgagg aagccgcgcc agttgtaggg cacgtaggcg tcactgaagg ggcggggcgc
     3181 ggcaccgccg agccacagcg gccagtcgag cgtggcggga atcggcgcct cgggcggcgg
     3241 cgcctgcaaa cccgtcggga aggtgccggg cgaggtggag acgtgcacct cgcgcacctc
     3301 gccaatctcc cccgtccaga cgatctcggc agccaccctg tgtgcctcgt gcgagaaccc
     3361 ctggttgccc atctgggtgg cgaccttgta cttcaccgcc gcgtcctgca gcagccgcgc
     3421 ctcccacggc gtcctcgtca gcggcttctc cacgtagacg tgcttcccgc gctccatgca
     3481 ccacagcgca acggtcgcgt gcatgaagtc gggaacggcg atcgtgcacg cgtcgatgtt
     3541 ccggccttcc ttgtcgagca tgacgcggaa gtccttgtac ctggggacgc cgccgtactt
     3601 cttgaagctg ccggcggcgc gtgcctcgtc cacgtcgcag agtgccacga ccgtctcggt
     3661 gcgggccgcc tggtcgagga tgcccgcgcc ctggccgccg cacccgatgg ccgccacccg
     3721 cagcttgctc gagaacggct ggtagccgag cgccttcagc gaggggacgc tgccgaaacc
     3781 ggcggccggg acagcgcccg cgagaagcgt cccgaagaag aagtgccttc tggaaatgga
     3841 gttgtcgctc atcgatcctc cgcggggtat cttacacgcc tcagccccac aattcaggaa
     3901 gcggcggctc gacgcggccg tggcggtaca ccatcgccgg ctcgcggtcc cgcacctgca
     3961 gcagcggtcc gtcgagatcc acgtactcgc agtgctgcgc cagcaggaag gcgggcgcca
     4021 tcgccagcga ggtgccgcac atgttgccga ccatcagccc caggccgagc gcccgggcgc
     4081 cctgcgccat ggccagcgcc tcggtgagcc cgccacactt gtcgagcttg atgttcacgt
     4141 aacggtagcg tcccgccagg cgcgcgagcg aacagcggtc ggtgcacgac tcgtcggcgg
     4201 cgagcgggat cgtcgagtcg aaaccgtcca ggcacgcgtc ctcgccgcgc gcgaccggct
     4261 gctcgatcag ctccacgccc agctcccgca acccaggcag cagcccctcg agccgctcgc
     4321 ggctccaggc ctggttcgcg tccacgatga tgcgggcgcg cgggtgctcc tctttcacga
     4381 tccgcacctg gtccacgtgg cggacgccgt ccaccttcag cttgagcagc gggtactccc
     4441 gcgcctcgcg tgccctgcgc cgcgtcgccg cctcgtcgcc cagcccgatc gtgtagtcgg
     4501 tcagcaccgg ccccatccca cgaaggccgg ctgccttcca cgcgcgccgg cccgactgct
     4561 tggcgcgcag gtcccacagc gcgcaatcga gcgcgttgcg cgcgccgccg gcgggcagca
     4621 gccgcagcag gtcgctgccg cgcagctcgt cgcgcagctc gccggccacc gcctcgatct
     4681 ggctcgccat cccctccggc gtctcgccgt cgtagtccac gccggccgcc tcggcgacgc
     4741 cctcggccca ccgcgtcgcc aggggcacca tccgggcga
//
LOCUS       DAOPQX010000289         4775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19468_length_4775_cov_4.221822, whole genome shotgun sequence.
ACCESSION   DAOPQX010000289 DAOPQX010000000
VERSION     DAOPQX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19468_length_4775_cov_4.221822"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKK95_13455"
     CDS             complement(<1..261)
                     /locus_tag="PKK95_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19273.1"
                     /translation="MTPPSQPSDSSTATAGTRTILAFWAPLAATWLMMAVEGPYIAAI
                     IARLAQPTPNLAAYGVAFSFAFIAEAPVIMMMTAANALVADRG"
     gene            355..1812
                     /locus_tag="PKK95_13460"
     CDS             355..1812
                     /locus_tag="PKK95_13460"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOC19274.1"
                     /translation="MKTIDLSLSNLNELFPADFSPEQLAKAKTAFLKELSLHAHKFYG
                     GKIVSMPKAGIYGFNWFNVWYTPGVSAVSTTIRDDHDSSFELSNRGNLVAVVSDSTRV
                     LGDGDCSPSGGLGVMEGKAFLMKYLGGVDGVALCVNSYNAAGKHDPDKIIDFVKMVEP
                     SFGAVNLEDISQPNCYKVLDTLREACDIPVWHDDAQGTGSVTLAGLINALRIVDKPID
                     QARIVFYGAGASNTTIARLILMAGADPARLVMFDTKGGLHKGRDDIQADPAFYRKWEL
                     CEQTNPDRINDIDQAVKGADVLIALSKPGPETVKAGWIRSMASKAILFACANPVPEIY
                     PYAAKDAGAYIVATGRGDFPNQVNNSMGFPGILKGALLVRARKITDEMAIAAAYAVAN
                     FAQEKGIHPDYIMPTMDETEVFAEEAADVAMTAIKNGVARTILDRETVYTRTLHDIQE
                     ARDMIGTLMKADFIKPPDASMLQAAVDKAVALVRG"
     gene            1993..2976
                     /locus_tag="PKK95_13465"
     CDS             1993..2976
                     /locus_tag="PKK95_13465"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC19275.1"
                     /translation="MLVLRALQLGDLLCAVPALRAMRAAWPSARIELVGLRWARAFVD
                     RFDRYLDGFVEFPGWPGLPEREPDLGAWPPFLEALRSRRTALALQMQGNGRITNALLA
                     RFGADETAGFVARGEAGPVPGLFVEYPEDRHELQRLLALTAALGVPSRGEHLELPLTE
                     RDEREWQALRADLGLKPQAYACLHPGARAEARRWPPEHFARVGEALLARGMTRVIVTG
                     DGEEETRLAGAVVEAMAGRATSVAGRPSLGALAALVRDARLIVCNDTGLSHVAAALRT
                     PSVVVFRITEPRRWAPLDRVRHRVVVDGLGAVGRAIAGAEALLAGEAPHAA"
     gene            2966..4066
                     /locus_tag="PKK95_13470"
     CDS             2966..4066
                     /locus_tag="PKK95_13470"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC19276.1"
                     /translation="MRPERGGETRRVLAVRLDNLGDVVMTGPAIRALRDRWPDASITL
                     LSSPAGAAAARMLPWIDEILPHRAVWQDASNALPLDPSREQAFIEALAARRFEAAFIF
                     TSFSQSPWPAAYACYLAGIPLRAGHSHGFGGSLLTVQVPPMPDSSHQVDRNAAVVEAA
                     GIAVADRRLDAIAPAGARESARRALAAIGIGKGDPFAVVTPGASCTARRYSPARFAEA
                     ASSLAGLLSCPVVITGSPREAALVEEVCLRAASPAVRLLGNVDICALAAVLAGARLLV
                     CNNSGPMHLADAVGCPMVVLFSGTDLEEQWRPRSAPAILLRRPTPCRPCYLFDCRHQM
                     ECLDVPPAEVVSAGLSLAAPTARTVPVAAGAE"
     gene            4078..>4775
                     /locus_tag="PKK95_13475"
     CDS             4078..>4775
                     /locus_tag="PKK95_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19277.1"
                     /translation="MRVLTWHVHGSYLYYLAHAPHDFYVPVKEGRPEGYGGRADGFDW
                     PANLHEVPADRVRDIEFDCVLFQSKRNYLVDQHEVLSGAQRRLPRVYLEHDPPREHPT
                     DTRHVVDDPEVLLVHVTCFNELMWDAGRTPTRVIEHGVVVPDGLAYRGDLARGLVVVN
                     GLASRGRRLGADVFERARREVPLDLAGIGSEGIGGLGEVGHAELPRLAVNYRFFFNPI
                     RYTSLGLAVCEAMTL"
BASE COUNT          676 a         1718 c         1663 g          718 t
ORIGIN      
        1 accccggtcg gcgacgagcg cgttggcggc ggtcatcatc atgatgaccg gcgcctcggc
       61 gatgaacgcg aacgagaagg cgacgccgta cgccgcgagg ttgggggtcg gctgggcgag
      121 gcgcgcgatg atggcggcga tgtaggggcc ttcgaccgcc atcatcagcc acgtcgcggc
      181 cagcggcgcc cagaacgcca ggatcgtccg ggtgccggca gttgcggtcg agctgtcgga
      241 gggctgcgag ggtggcgtca cacgcgggct caggccccca ttctagccgg tttgtgtgct
      301 atcctccggc cgtccatccc agggcgggat ccccacatcc tcgcggagac gatcatgaag
      361 acgatcgact tgtcgctctc gaatttgaac gagctgttcc ctgccgactt cagcccggag
      421 cagctggcga aggcgaagac cgccttcctc aaggagctct cgctccacgc gcacaagttc
      481 tacggcggca agatcgtctc gatgcccaag gctggtatct acggcttcaa ctggttcaac
      541 gtctggtaca cgcccggcgt ctcggcggtc tccaccacca tccgcgacga ccacgattcc
      601 tcgttcgagc tgagcaatcg cggcaacctg gtagcggtcg tctccgactc gacccgcgtg
      661 ctgggcgacg gcgactgctc gccctcgggc ggcctcggcg tcatggaggg caaggccttc
      721 ctcatgaagt acctcggcgg ggtggacggc gtcgccctct gcgtcaacag ctacaacgcc
      781 gccggcaagc acgaccccga caagatcatc gacttcgtga agatggtcga gccgagcttc
      841 ggggccgtga acctggagga catctcccag ccgaactgct acaaggtgct cgacacgctg
      901 cgcgaggcgt gcgacatccc ggtgtggcac gacgacgcgc agggcaccgg cagcgtgacg
      961 ctggccggcc tgatcaacgc gctgcgaatc gtggacaagc ccatcgacca ggcgcggatc
     1021 gtgttctacg gcgccggcgc ctcgaacacg acgattgccc ggttgatcct gatggcgggc
     1081 gccgacccgg cccggctcgt gatgttcgac acgaagggcg gcctccacaa gggccgcgac
     1141 gacatccagg ccgaccccgc gttctaccgc aagtgggagc tctgcgagca gaccaacccg
     1201 gaccggatca acgacatcga tcaggcggtg aagggcgccg acgtgctgat cgccctcagc
     1261 aagcccggcc ccgagacggt gaaggcgggc tggatccgct cgatggcgag caaggcgatc
     1321 ctctttgcgt gcgccaaccc ggtgcccgag atctacccgt acgccgccaa ggacgccggc
     1381 gcctacatcg tggccaccgg gcgcggcgac ttcccgaacc aggtcaacaa ctcgatgggc
     1441 ttccccggga tcctcaaggg cgcgctgctc gtccgggccc gcaagatcac cgatgagatg
     1501 gcgatcgcgg ccgcctacgc ggtggcgaac ttcgcccagg agaagggcat ccaccccgac
     1561 tacatcatgc ccacgatgga cgagacggag gtcttcgccg aggaggccgc cgacgtggcc
     1621 atgacggcga tcaagaacgg cgtggcccgc accatcctcg accgtgagac cgtctacact
     1681 cgcacgctcc acgacatcca ggaggcgcgc gacatgatcg gcacgctcat gaaggccgac
     1741 ttcatcaagc cgcccgacgc ctccatgctt caggccgccg tggacaaggc ggtcgcactg
     1801 gtgcgaggct agccggccgg cgctcctcgt cctgtccctt cccgacgcaa ctcgatggcg
     1861 tgcgccgccg aaaagcggtg acgccgtgcc gtaccgcgcc cgcgacaccg cgaccgtatc
     1921 ccgccgttcg cagtggcggc tctcttgcac cccgcctgag gcgtggcaca cggcagatca
     1981 ataccgcgat cggtgctcgt gctgcgcgcg ctccagctcg gcgacctcct gtgcgccgtg
     2041 ccggcgttgc gcgcgatgcg tgccgcgtgg ccgtcggcgc gaatcgagct ggtggggttg
     2101 agatgggctc gggccttcgt cgatcgcttc gacaggtacc tcgacggctt cgtcgagttt
     2161 cccggctggc ccggcctgcc cgaacgggag ccagacctcg gtgcctggcc gcccttcctc
     2221 gaggcgctgc ggtctcgccg caccgccctg gctctccaga tgcagggaaa cggccgcatc
     2281 accaacgcgc tcctcgcgcg cttcggcgcc gacgagacgg ccggcttcgt cgctcgcggc
     2341 gaggccggcc ccgtgccggg cctctttgtc gagtaccccg aggaccgtca cgagcttcag
     2401 cggctgctcg cgctcaccgc ggcgctcggc gtcccctccc gcggcgagca cctcgaactc
     2461 ccgctcaccg aacgcgacga gcgcgagtgg caggcgctgc gtgccgacct gggcctgaag
     2521 ccccaggcct acgcgtgcct ccacccgggt gcgcgggccg aggcgcggcg ctggccaccc
     2581 gagcacttcg cgcgggtcgg cgaggcgctc cttgcacgtg gaatgacgcg cgtgatcgtc
     2641 acgggtgacg gcgaggagga gacgcgcctg gccggggcgg tggtcgaggc aatggcggga
     2701 cgggcgacga gcgttgccgg ccgcccgtcg ctcggcgcgc ttgccgcgct cgtccgcgac
     2761 gcgcggctca tcgtctgcaa cgacaccggc ctctcgcacg tcgcggccgc ccttcgcacg
     2821 ccgagcgtcg tcgtcttccg catcaccgag ccgcggcgct gggcgccgct cgaccgggtg
     2881 cgccaccggg tcgttgtcga cggcctgggg gccgtgggcc gcgcgattgc gggcgccgag
     2941 gcgctgctcg ccggggaggc cccgcatgcg gcctgagcgt gggggagaga cgcggcgggt
     3001 tctcgcggtg cgcctcgaca acctcgggga cgtcgtgatg accgggccgg cgattcgcgc
     3061 gctgcgggac cgctggcccg acgcgtcgat cacgctcctg tcgtccccgg caggggccgc
     3121 cgccgcgcgg atgctgccgt ggatcgacga gatcctcccg caccgggcgg tctggcagga
     3181 tgcgtcgaac gcgcttcccc tcgatccatc ccgggagcag gcgttcatag aggctctggc
     3241 ggcccgccgc ttcgaggcgg ccttcatctt caccagcttc tcgcagtcgc cgtggccggc
     3301 cgcgtacgcc tgctacctgg ccggcatccc gctgcgagcg ggtcactcgc acggcttcgg
     3361 cgggagcctg ctcaccgtcc aggttccgcc catgcccgat tcgtcgcacc aggtggatcg
     3421 gaacgcggcc gttgtcgagg ctgcgggcat cgccgtcgcc gaccggcgcc tggacgcgat
     3481 cgccccggcg ggcgcaaggg agtcggcgcg gcgggcgctc gcggcgattg gcatcggcaa
     3541 aggcgacccg ttcgcggtcg tgacgccagg ggcgagctgc acggcgcgcc gctacagccc
     3601 cgctcgcttc gcggaggctg cgtcgagcct ggccgggctc ctgtcctgcc ccgtggtcat
     3661 caccggcagc ccgcgcgaag ccgcacttgt cgaggaagtg tgcctgcgcg cggcgtcgcc
     3721 cgccgtgcgg ttgctcggca acgtggacat ctgcgcgctc gccgcggttc tcgccggcgc
     3781 gcggctgctg gtctgcaaca actcggggcc catgcacctc gccgacgccg tcggctgccc
     3841 gatggtcgtt ctgttctcgg ggacggacct cgaggagcag tggcggccgc gctcggcgcc
     3901 cgcgatcctg ctgcgccggc cgacgccctg ccgcccctgc tacctcttcg actgccgcca
     3961 ccagatggag tgcctcgacg tgccgcccgc cgaggtggtg tcggcggggc tgtcgctcgc
     4021 cgcgccaacg gctcgcacgg tgcccgttgc agcgggggcg gagtgactga ggcgacgatg
     4081 agagtcctga cctggcacgt gcacgggagc tacctttact atctcgccca cgctccgcac
     4141 gacttctacg tgccggtgaa ggaggggcgg cccgaggggt acggcgggcg cgccgacggc
     4201 ttcgactggc cggcgaacct gcacgaggtg ccggccgacc gcgtccgcga catcgagttc
     4261 gactgcgtcc tcttccagtc gaaacgcaac tacctggtgg accagcacga ggtcctctcc
     4321 ggagcgcagc gccgccttcc gcgcgtctac ctggagcacg accctcctcg cgagcacccg
     4381 accgacacgc gacacgtcgt agacgacccg gaagtgctgc tcgttcacgt gacgtgcttc
     4441 aacgagctga tgtgggacgc ggggcgcacg ccgacgcgcg tgatcgagca cggcgtggta
     4501 gtgccggacg ggctcgcgta tcggggcgac ctcgcccgcg ggctcgtggt cgtcaacggc
     4561 ctcgcctccc gcgggcgccg gctcggggcg gacgtgttcg agcgggcgcg gcgcgaggtg
     4621 ccgctcgacc tggccggcat cgggtcggaa gggatcggcg ggctcgggga ggtcggccac
     4681 gccgagcttc cgcgactcgc ggtcaactac cgcttcttct tcaacccaat ccggtacacc
     4741 agcctgggcc tcgcggtctg cgaggcgatg acgct
//
LOCUS       DAOPQX010000290         4775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19469_length_4775_cov_3.797246, whole genome shotgun sequence.
ACCESSION   DAOPQX010000290 DAOPQX010000000
VERSION     DAOPQX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19469_length_4775_cov_3.797246"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..390)
                     /locus_tag="PKK95_13480"
     CDS             complement(88..390)
                     /locus_tag="PKK95_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOC19278.1"
                     /translation="MKPALGMVETRGLVALFEAVDAMLKAATVTFTAWEPVGSGLVTA
                     FVEGDVAAVKAATDAGAEAARKLGEVVSVQVIPRPHDDLNQLLAQFGPKAGTAGAA"
     gene            complement(387..1070)
                     /gene="pduL"
                     /locus_tag="PKK95_13485"
     CDS             complement(387..1070)
                     /gene="pduL"
                     /locus_tag="PKK95_13485"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: protein motif:HMM:NF011652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOC19279.1"
                     /translation="MAREPGHAVSRGLVEQIVREVVTSRLAELTGGASSRLVVQASSR
                     HAHLSREHLEALFGPGYELTPERPLYQEGNYAAREQVTLVGPRGRTLQLRVLGPTRGR
                     SQIELAFTDAITLGFENVPVRLSGDIDGTPGAHVLGPKGMVRLEEGLIRAAIHVHMNP
                     DEARHYGVQQGDLMQLRVGGDAGVVFSRVHVRIDPRSRLNVHMDTDEANACALHLASE
                     MELFTEKKR"
     gene            complement(1060..2232)
                     /locus_tag="PKK95_13490"
     CDS             complement(1060..2232)
                     /locus_tag="PKK95_13490"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19280.1"
                     /translation="MNILVANIGSTSFKYRLYRTEAGAITPVAHGRVERIGQPGSPCS
                     DYESAIRLCTDDIAGEGRALAGLSDLSAIGFKAVHAGPVSGARVVDDEVLEAMEAFAF
                     FAPAHNPPYVAAMRSFRRALPGVPLVALFETAFFDALDEAATTYAVPYAWRQDLGVRR
                     YGFHGASHRAASEHARSIVGPKARHISCHLGGSSSVAAIRDGVAVDSSFGMSPQSGLP
                     QNNRTGDLDVFAALFVMKRRGLGVDEMARVLASESGLAGLSGLSGDLRDLDEAASSGN
                     PRARLALDVYVRAIRHSVGAFLVELGGLDVLTFSGGIGENSPAIRAGVCRGLEAFGIV
                     LDEGVNAATRAEARISPAGSPVAVLVVPADEERIVARATAEVIAGLPAPAGGAHGA"
     gene            2487..3818
                     /locus_tag="PKK95_13495"
     CDS             2487..3818
                     /locus_tag="PKK95_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOC19281.1"
                     /translation="MAKASGEACGGIPDVTRLEALGIVGAGGGGFPAAVKLRVKVPTV
                     IVNGAECEPLLHKDKELLQHQAGPMLRGLDVAMALVGAREGVVGVKEKYRAVLEALGP
                     QLRAGVRLLPLRDTYPAGDEFVLVHDATGRVIPPGGLPKDVGAVVLNVETLVNIGLGL
                     PVTHKYLTVAGAVGEPVTLRVPVGMSIGEAIEAAGGPTEPNVAALLGGVMMGRLATGF
                     DEPVTKTLGGIVVLPAAHKLVTWYKTGRRQVERIGKSACDQCRFCTELCPRYLLGHPI
                     EPHKAMQALGFTGQAARLVAGTLYCCECNLCSLYSCPEDLDPKNVCTFGKPVARQAGL
                     AWQGDPASVAPHPLGSARRVPMRRLMTRLGLNGFANAGPLADREFHPRRVVLPLKQHA
                     GAPAVPRVAAGTRVAPGQVIAAPAPGALGATLHASIAGVVREVGEAIVIEA"
     gene            3848..>4206
                     /locus_tag="PKK95_13500"
     CDS             3848..>4206
                     /locus_tag="PKK95_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOC19282.1"
                     /translation="MAKLNSIGLVETSSIAVGYRAEDAMLKAAEVQLLLARTICSGKY
                     LVVVGGDVASVQASVEAGAAQCNGALIEQRVVTQVHPGVFPAIGLSVEPGPDRIQALG
                     VIEGADEISIEFFSGEKL"
     assembly_gap    4207..4216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4217..4489
                     /locus_tag="PKK95_13505"
     CDS             <4217..4489
                     /locus_tag="PKK95_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOC19283.1"
                     /translation="VTSTRLEARTDSASSIVEVADAAAKAADVVLFRVHLAMAIGGKG
                     FVLLTGDVASVEAAVAAGAAVAADEGILVAKTVIPAPARELFTELI"
     gene            complement(4499..>4775)
                     /locus_tag="PKK95_13510"
     CDS             complement(4499..>4775)
                     /locus_tag="PKK95_13510"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOC19284.1"
                     /translation="LDAIAAKDAAGTLWLALTNVDPNRPATIAVRLAGARAATASGET
                     LTAGAVDSVNTFEAPNAVVPKPITAKVEGDRLLLTLEPKSVTVVSVR"
BASE COUNT          699 a         1624 c         1769 g          673 t
ORIGIN      
        1 gtgcttttcg cgcgaagccg agcgggggga gccccgcggc aggacccgcg aacacgcacg
       61 cttcctccgt catggtgaag aagagggcta ggccgccccc gcggtcccgg ccttcggccc
      121 gaactgcgcg agcaactggt tgaggtcgtc gtggggacgc gggatcacct ggacgctgac
      181 cacctcgccc agcttccgcg ccgcctcggc gcccgcgtcg gtcgccgcct tgaccgctgc
      241 gacgtccccc tcgacgaagg cagtgaccag gcccgagccc accggctccc aggccgtgaa
      301 ggtgacggtg gccgccttca gcatcgcgtc caccgcctcg aagagcgcca cgagcccccg
      361 cgtctcgacc atgcccagcg ctggtttcat cgtttcttct ccgtgaacag ttccatctca
      421 ctggccaggt gcagcgcgca cgcgttggcc tcgtcggtgt ccatgtggac gttgagccgg
      481 gaacgcgggt cgatccgcac gtgcacccgg ctgaagacaa ccccggcatc gccgccaacc
      541 cgcagctgca tcaggtcgcc ctgctgcacc ccgtagtggc gggcctcgtc cgggttcatg
      601 tggacgtgaa tcgccgcccg gatcaggccc tcctccaggc gcaccatccc cttgggcccg
      661 agcacgtgag caccgggcgt cccgtcgatg tcgcccgaca gccgcaccgg cacgttctcg
      721 aagccgaggg tgatggcgtc ggtgaaggcc agctcgatct gcgaacggcc gcgggtcggg
      781 ccgaggacgc gaagttgcag agtccggccg cgcgggccga ccagcgtcac ctgttccctg
      841 gcggcgtagt tgccttcctg gtagagcggg cgctcgggcg tcagctcgta cccggggccg
      901 aagagcgcct cgaggtgctc gcggctcagg tgcgcgtggc gcgacgaggc ctggacgacc
      961 aggcgcgacg aggcgccgcc cgtcagctcg gcgaggcggc tcgtcaccac ctcgcgcacg
     1021 atctgctcga ccagcccgcg gctgaccgcg tgccccggct cacgcgccat gagcccctcc
     1081 cgccggcgcc ggcaggcccg cgatcacctc ggcggtggcg cgcgccacga tccgctcctc
     1141 gtcggccggc acgacgagca cggcgacggg ggagcccgcg ggtgagatcc gcgcctcggc
     1201 gcgggtcgcc gcgttgaccc cctcgtcgag gacgatgccg aaggcctcga ggccgcggca
     1261 gacgcccgcg cggattgccg ggctgttctc gccaatgcca cccgagaagg tgagcacgtc
     1321 gaggccgccc agctcgacga ggaacgcccc gacggagtgg cggatggcac gcacgtacac
     1381 gtcgagggcc aggcgggcac gcgggttccc cgacgaggcc gcctcgtcga gatcgcgaag
     1441 gtccccgctc aggccgctga ggcccgcgag ccccgattcg gacgcgagca cgcgggccat
     1501 ctcgtccacc ccgaggccgc ggcgtttcat gacgaagagg gccgcgaaca cgtcgaggtc
     1561 gccggtgcgg ttgttctggg ggaggcccga ctgcggcgac atcccgaagc tgctgtccac
     1621 cgcgacgccg tcgcggatgg cggcgacgct ggagctgcca ccgaggtgac acgagatgtg
     1681 gcgggccttc ggcccgacga tcgagcgggc gtgctcgctg gccgcacggt ggctggcgcc
     1741 gtggaagcca tagcggcgaa cgccaaggtc ctggcgccag gcgtagggaa ccgcgtaggt
     1801 ggtggcggcc tcgtccaggg cgtcgaagaa ggcggtctcg aagagcgcga cgagggggac
     1861 gccgggcagc gcgcgccgga acgaacgcat ggccgccacg tagggcgggt tgtgcgccgg
     1921 agcgaagaag gcaaatgcct ccatcgcctc cagcacctcg tcgtccacca cccgggcgcc
     1981 gctgacgggg cccgcgtgca cggccttgaa gccgatggcg gaaaggtccg acaggccggc
     2041 cagcgcgcgt ccctcgccgg cgatgtcatc ggtgcacagg cggatggcgg actcgtaatc
     2101 ggaacagggg cttcccggct ggccaatccg ctcgacgcgc ccgtgggcaa cgggagtgat
     2161 cgcgcccgcc tcggtccggt agaggcggta cttgaaggag gtgctgccga tgttggcgac
     2221 caggatgttc atggatgaca gaaaatgaaa gaaatctgtc actcgttccg ggtgcatcga
     2281 acgacaacga gcgctgggac gaagcagcct atctctacca attatcggga gtctggccgc
     2341 cttcctttat ccgcgtccgg cgggtctctc cctgcaggcg aggcctgttg cggccgaatc
     2401 atatgaaagg gacttgacag aagtcaacag tattcttaca gttcctggcg acaatctggc
     2461 ggacaacggt ctcaggcaag aggactatgg ccaaggccag cggcgaggct tgcggcggca
     2521 tcccggacgt gacccggctc gaggcgcttg gcatcgtcgg cgcgggcggg ggagggttcc
     2581 cggccgccgt caagctgcgg gtgaaggtgc cgaccgtcat cgtcaacggc gccgagtgcg
     2641 agcccctgct gcacaaggac aaggaactgc tgcagcacca ggcgggcccg atgctgcggg
     2701 gcctggacgt cgcgatggcg ctggtgggcg cgcgcgaagg ggtcgtcggg gtgaaggaga
     2761 agtaccgggc ggtgctcgag gcgctcgggc cccagctgcg cgccggcgtg cggctgctgc
     2821 cgcttcgcga cacctacccg gcgggcgatg agttcgtgct ggtgcacgac gcgaccggcc
     2881 gcgtcatccc gcccggcggg ttgccgaagg atgtcggcgc ggtggtgctg aacgtcgaga
     2941 cgctggtcaa catcggcctg ggtctccccg tcacgcacaa gtacttgacg gtggcgggcg
     3001 ccgttggcga gccggtcacg ctgcgggtcc cggtggggat gtcgatcggc gaggcgatcg
     3061 aggcggccgg cgggccaacc gagccgaacg tcgcggcgct gctcggtggc gtcatgatgg
     3121 ggcgcctggc gacgggcttc gacgagccgg tcaccaagac gctcggcggg atcgtcgtgc
     3181 tgccggccgc ccacaagctc gtgacctggt acaagacggg ccgccggcag gtcgagcgca
     3241 tcgggaagtc ggcgtgcgac cagtgccggt tctgcaccga gctgtgcccg cgctacctgc
     3301 tcggccaccc gatcgagccg cacaaggcga tgcaggccct cggcttcacc ggccaggcgg
     3361 cgaggctggt ggccggcacg ctgtactgct gcgagtgcaa cctctgcagc ctctactcct
     3421 gtcccgagga cctcgacccc aagaacgtct gcaccttcgg gaagccggtg gctcggcagg
     3481 cgggcctggc ctggcagggc gacccggcgt cggtcgcccc gcacccgctc gggagcgcgc
     3541 ggcgggtgcc gatgcggcgc ctcatgacga ggctcggctt gaacggcttc gcgaacgcgg
     3601 ggccgctcgc cgatcgggag ttccacccgc ggcgcgtcgt gctgccgctc aagcagcacg
     3661 caggcgcgcc ggcggtgccg cgggtcgccg ccgggacgcg cgtcgcgccc ggccaggtga
     3721 tcgccgcacc ggcgcccggg gcgctcgggg cgacgcttca cgcgagcatc gccggggtgg
     3781 tgcgcgaggt tggcgaggcg atcgtcatcg aggcgtgagc ggcggggctg ctgaaaggac
     3841 ggacgcggtg gcaaagctca attccatcgg actcgtcgag acctcctcga tcgcggtcgg
     3901 ctaccgggcc gaggacgcca tgctcaaggc ggccgaggtg cagctgctgc tggcccgcac
     3961 catctgctcg ggcaagtacc tcgtggtcgt cggcggcgac gtcgcgtcgg tgcaggcctc
     4021 ggtggaggcg ggcgcggcgc agtgcaacgg ggcgctcatc gagcagcgcg tcgtcacgca
     4081 ggtccacccc ggcgtcttcc cggccatcgg cttgtcggtg gagccgggcc ccgaccgcat
     4141 ccaggcgctc ggcgtcatcg agggcgcgga cgagatctcg atcgagttct tctcgggcga
     4201 gaagctnnnn nnnnnngtga cgagcacgcg gctcgaggcc cgtaccgact cggcctcgag
     4261 catcgtcgag gtggccgacg cggccgcaaa ggccgccgac gtcgtgctgt tccgcgtgca
     4321 cttggcgatg gcgatcggcg gcaaggggtt cgtgctgctg acgggcgacg tggcgagcgt
     4381 ggaagcggcg gtcgcggcgg gggccgcggt ggcggccgac gaggggattc tcgtcgcgaa
     4441 gacggtcatc cccgcgccgg cccgggagct gttcacagag ctgatctagg cgatccaatc
     4501 accggaccga gaccacggtc accgacttgg gctcgagggt caggaggagc cgatccccct
     4561 ccaccttcgc cgtaatcggt ttcggcacca cggcgttcgg cgcctcgaag gtgttcacgc
     4621 tgtccacggc gccggcagtc agcgtttcac cggaagcggt ggcggcacgg gcgccggcaa
     4681 ggcggacggc gatcgtggcc gggcggttgg gatcgacgtt ggtcagcgcg agccacaggg
     4741 tgccggccgc gtccttcgcg gcgatcgcgt ccagg
//
LOCUS       DAOPQX010000291         4749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19602_length_4749_cov_4.874095, whole genome shotgun sequence.
ACCESSION   DAOPQX010000291 DAOPQX010000000
VERSION     DAOPQX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19602_length_4749_cov_4.874095"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1147
                     /locus_tag="PKK95_13515"
     CDS             224..1147
                     /locus_tag="PKK95_13515"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOC19285.1"
                     /translation="MRRVFLAGAESAWLRGRAMRAPFVRRSVSRFMPGETMDDALEAA
                     RQIEAQQGTGALLTHLGENLSSLAEAEAVTGHYLRLIDRVREGRLDSQVSVKLTQLGL
                     DLGAAECLENVRRLVEHAERAGSMVWIDMESSPYVDPTLDIFRRLRERTPRVGVCLQA
                     YLRRTPSDLDTLLPVGAAIRLVKGAYREPPAVAFPKKADVDEQYFRLARRLLDPSLPQ
                     GTFLGIATHDERLIERLRTTAAAGGADPARYEHEMLYGIQRPLQAKLTHEGERLRILI
                     SYGEYWFPWFMRRLAERPANAFFVARNLLRG"
     gene            1232..2212
                     /locus_tag="PKK95_13520"
     CDS             1232..2212
                     /locus_tag="PKK95_13520"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOC19286.1"
                     /translation="MSSDRPAISRRGLLTGVLGAAAASMTARRAAAQGRARVIRVESP
                     KVWAADRRDPAAVAAMVDRGLTAFTGKAKPAEAWAQFFKPGMRVGLKINLLGRPLLYT
                     APEVTDAVVAGVLSAGVKPADVIVWDRHADHFGPTRYKADTTGARGERIMTGGRYDRS
                     RACEASGGLAPIDRIATELTDMTVNLPLLKNHGMAGVTLALKNIAFGCYQHHRAAHSG
                     NCDPFVAEACRHYLSQTKVPLHVLDATEACYDQGPQPGSTEPIWRENAIYVATDPVAL
                     DVVCREVILARQRASGLGNRMSQCRHIETAASMGLGVGDPGRIDLVTIRV"
     assembly_gap    2287..2386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2387..3213)
                     /locus_tag="PKK95_13525"
     CDS             complement(<2387..3213)
                     /locus_tag="PKK95_13525"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HOC19287.1"
                     /translation="MPEGDTIFRSARTLKAALAGRSVTAFESRFASLDRHVVDRPLTG
                     QTVLDVRAAGKHLLMEFSGGLVLRTHMRMNGSWHLYRPGERWRRARSAMSVLVATGEF
                     VAVAFDVPVAEFVPAARLASHRPLSRLGPDLLSESFDIDEAAARLSARPGETIARALI
                     DQAVMAGAGNVYKSEALFLSAIHPGRRVSTLSPEEVRRLIGTLRSLMLANVVEPSERS
                     FGPAFPGYRRTTRLANPAARLWVYGRTGRPCRRCGTPIRYAKQGEEARGTYWCEKCQR
                     "
     gene            complement(3247..>4749)
                     /locus_tag="PKK95_13530"
     CDS             complement(3247..>4749)
                     /locus_tag="PKK95_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19288.1"
                     /translation="PAGDLVLRFARTHGPFTIEEAAARFGLAPRTVEAALARLAARGR
                     VLQGAFRPARRGREWCESEVLRQIRQRSLARLRRQVEPVSAAALGRLSVAWHGVTAPR
                     RGLDALLDVVEQLQGLPLAASLLEREILPARLAEFEPSQLDALCSAGEIAWVGLEPLG
                     ERDGRLALYLTDHLPRLLPPGTPGPELDDRARRIVEFLSRAGASFFGAIHEACGGGYP
                     GETVDRLWDLAWMGLVTNDSFRALRALTRPPERRRRRPDPAPFRSRRLTPPRAEGRWS
                     LVPRPAAPGKRAATAWASDMATQLLTRHGIVTREIVAVENLPGGFSAIYQVLKAMEDA
                     GRVRRGYFVGGLGATQFALPGAVDLLRSLRDEPDEPQAVLLAATDPANPYGAVLRWPA
                     SASESGSRGPTRSVGARVVLVNGTLAAYVGKADRLFLSFLPEEEPARGIAARAVARLW
                     FEMATAGPEGMLIGEIDGQVVERHALAPFLLEAGFTRRATGFQAAKERTS"
BASE COUNT          678 a         1721 c         1630 g          620 t
ORIGIN      
        1 gcctgccgtc acctctgtcg ctcatgtggt cgcccccgga tcatcctgga cttcaccact
       61 ttctcacaca tggccgcggc ggcccatccc ggggcgatgc gaggacgtcc ccggttcgag
      121 atccggcccg gttttcgggc cgccgcaacc gcgtcggctg ccgcgcctga ggtacaatac
      181 cggcttcgca cacaacgcgg cgcaggggaa ctccatgtca ctcatgagac gcgtgttcct
      241 cgccggggcc gagagcgcgt ggctgcgtgg gcgcgccatg cgggcaccct tcgtccggcg
      301 ctcggtctcg cgcttcatgc cgggcgagac gatggacgac gccctcgagg cggcgcggca
      361 gatcgaggcg cagcagggaa ccggcgcgtt actcacgcac cttggcgaga acctgtcgtc
      421 gctcgccgag gccgaggcgg tcaccgggca ctacctccgg ttgatcgacc gcgtgcgcga
      481 gggccggctc gattcccagg tgtcggtgaa gctcacccag ctcggcctcg acctcggcgc
      541 ggccgagtgc ctcgagaacg ttcgccgcct cgtcgagcac gccgagcggg ccggcagcat
      601 ggtctggatc gacatggaaa gctcgccgta cgtcgatccg acgctcgaca tcttccggcg
      661 cctcagggag cgcacaccgc gcgtcggcgt ctgcctgcag gcctacctgc ggcgcacccc
      721 gtccgacctc gacacgctgc tcccggtcgg cgccgccatc cgcctggtca agggtgccta
      781 ccgcgagccg ccggcggtgg ccttcccaaa gaaggccgac gtggacgagc agtactttcg
      841 gctggcgcga cggctcctcg acccatcgct ccctcagggc acgttccttg gcatcgccac
      901 gcacgacgag cggctcatcg agcggctgcg cacgaccgcg gcagcggggg gggccgaccc
      961 ggcacggtac gagcacgaga tgctgtacgg catccagcgc ccgctccagg cgaagctgac
     1021 acatgagggc gagcgtctcc gcatcctcat cagctacggc gagtactggt ttccctggtt
     1081 catgcgccgg ctcgccgagc ggcccgccaa cgccttcttc gttgccagga acctgctgcg
     1141 cggatagagt gacacgacag ccgcggccgg cgtccggctc ccaaatcccg attcctgaat
     1201 cccgaatcct gattcccaaa cggagtttcc catgtcgagt gaccgtcctg cgatatcgcg
     1261 tcgtggcctg ctgaccgggg tgctgggggc cgcggcggcg tcgatgaccg cgcgccgcgc
     1321 ggccgcgcaa ggacgggcgc gcgtgatccg cgtcgagtcg ccgaaggtgt gggccgccga
     1381 ccggcgagac ccggcggcgg tggccgcgat ggtggaccgg ggcctgacgg cgttcacggg
     1441 gaaggcgaag cccgccgagg cgtgggccca gttcttcaag ccaggcatgc gcgtcgggct
     1501 caagatcaac ctgctcggcc ggccgctgct ctacacggca cccgaggtga ccgacgcggt
     1561 cgtggccggc gtgctctccg ccggcgtgaa gccggccgac gtcatcgtct gggaccgtca
     1621 cgccgatcac ttcggcccga cgcgctacaa ggctgacacg accggcgcac ggggcgagcg
     1681 gatcatgacc ggcgggcggt acgaccgttc gcgcgcctgc gaggcgtcgg gcggcctcgc
     1741 gccgatcgac cggatcgcca ccgagttgac cgacatgacc gtgaacctcc cgctgctgaa
     1801 gaaccacggg atggcgggcg tcacgctggc cttgaagaac atcgcgttcg ggtgctacca
     1861 gcaccaccgt gccgcgcact cgggcaactg cgacccgttt gtcgccgagg cgtgccgcca
     1921 ctacctgtcc cagacgaagg tgcccctcca tgttctcgac gccaccgagg cctgctacga
     1981 ccaggggccc cagccgggca gcaccgaacc catctggcgc gagaacgcca tctacgtcgc
     2041 taccgatccc gtcgccctcg acgtagtctg ccgggaggtg atcctcgcca ggcagcgagc
     2101 ctccggcctc ggcaaccgga tgtcgcagtg ccgccacatc gaaaccgccg cgtcgatggg
     2161 gctgggggtg ggcgatcccg gccggatcga cctggtgacc atcagggtgt agggctagcc
     2221 gctgtcgcta ccgctggcac ttctcgcacc agtacgtccc ccgcgcctcc tcgccctgct
     2281 tcgcgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgct ggcacttctc
     2401 gcaccagtac gtcccccgcg cctcctcgcc ctgcttcgcg tagcggatgg gcgtgccgca
     2461 gcggcggcac ggacggcccg tgcggccgta gacccacagc ctcgcggccg ggtttgccag
     2521 ccgcgtcgtc cgcctgtatc cggggaatgc cggaccgaag ctgcgctccg agggctccac
     2581 gacgtttgcg agcatgagcg aacgaagcgt gccgatgaga cgccggacct cctccggcga
     2641 gagcgtcgag acgcggcggc cggggtggat ggccgagagg aacagcgcct ccgacttgta
     2701 gacgttgccc gcacccgcca ttaccgcctg gtcgatgagc gcgcgggcga tcgtctcgcc
     2761 cggccgcgcc gagaggcgcg cggccgcctc gtcgatgtcg aacgattcgg acagcaggtc
     2821 ggggccgagg cgagagagcg gccggtggga ggcgagacgc gccgcgggga cgaactccgc
     2881 aaccggcacg tcgaacgcga cggcgacgaa ctcaccggtg gcgacgagca ccgacatcgc
     2941 cgatcgcgca cgccgccacc gttcgcctgg gcgatagagg tgccagctcc cgttcatgcg
     3001 catgtgcgtg cgcaggacga gcccgcccga gaactccatc agcaggtgct tgccggcggc
     3061 ccgcacgtcg agcacggtct gccccgtcag cggccggtcg acgacgtgcc gatcgagcga
     3121 ggcgaaacgc gactcgaacg ccgtgaccga acgccccgcg agcgcggcct tgagcgtgcg
     3181 cgccgacctg aagatcgtgt cgccctcggg catctctcag cgtcgggggc gacccaaggg
     3241 tcgcccctac gatgtccttt ccttcgccgc ctggaacccc gtcgcccgtc gcgtgaagcc
     3301 ggcctcgagc aggaacggcg cgagcgcgtg gcgctcgacc acctggccgt cgatctcgcc
     3361 gatcagcatc ccctcggggc ccgcggtcgc catctcgaac cacaggcggg ccaccgcgcg
     3421 ggcggcaatg ccgcgcgccg gttcctcctc gggcaggaac gagaggaaga gccggtcggc
     3481 cttgccgacg taggcggcca gcgtcccgtt caccagcacg acccgcgcgc cgaccgagcg
     3541 ggtggggccg cgcgatcccg attcggatgc cgaagcgggc caccgcagca cggccccata
     3601 tgggttggcc gggtcggttg ccgccagcag caccgcctgg ggctcatcgg gctcgtcacg
     3661 cagcgagcgc agcaggtcca ccgcgccggg cagcgcgaac tgcgtggcgc ccagcccgcc
     3721 gacgaagtag ccacggcgca cgcgcccggc gtcctccatc gccttcagca cctggtagat
     3781 tgcgctgaag ccgcctggca ggttctcgac cgccacgatc tcgcgggtga cgatcccgtg
     3841 ccgcgtgagc agctgcgtcg ccatgtcgct cgcccaggcg gtggccgccc gcttgccggg
     3901 cgccgccggg cgcgggacga gcgaccagcg cccctcggcg cgcggcggcg tcaggcgccg
     3961 cgaccggaac ggtgccggat cgggacgccg cctgcggcgc tcgggcggcc gcgtgagcgc
     4021 tcgcagcgcc cggaacgagt cgttggtgac caggcccatc caggcgaggt cccagagacg
     4081 gtccaccgtc tcgcccgggt agccgccgcc gcacgcctcg tggatggcgc cgaagaacga
     4141 ggcgccggcg cgcgagagga actcgacgat ccgccgcgcc ctgtcgtcca gctcgggccc
     4201 gggagtgccg ggcggcagca ggcgagggag gtggtccgtc aggtacagcg ccaggcggcc
     4261 gtcgcgctcg ccgagcggct cgagtccgac ccacgcaatc tctcccgcgc tgcacaaggc
     4321 gtccagttgc gacggctcga actccgcgag ccgcgccggc aggatctccc gctcgagcag
     4381 cgacgctgcc aggggcaggc cctgcagctg ctcgacgacg tcgagcaggg cgtcgagccc
     4441 acggcggggc gcggtaaccc cgtgccaggc aaccgagaga cggccgagcg cggcggcgct
     4501 gaccggctcg acctgccgcc tcaggcgcgc gagcgaccgc tgccggatct gccgcaggac
     4561 ttcgctctcg caccactcgc gcccgcgccg ggcgggtctg aaggccccct gcagcacgcg
     4621 cccccgcgcg gcgaggcggg caagcgccgc ttcgacggtt cggggggcga ggccgaagcg
     4681 cgcggccgcc tcctcgatgg tgaacgggcc gtgcgtccgc gcgaaacgca gcacgagatc
     4741 gccggccgg
//
LOCUS       DAOPQX010000292         4744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19626_length_4744_cov_4.612711, whole genome shotgun sequence.
ACCESSION   DAOPQX010000292 DAOPQX010000000
VERSION     DAOPQX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19626_length_4744_cov_4.612711"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /gene="metG"
                     /locus_tag="PKK95_13535"
     CDS             complement(<1..1144)
                     /gene="metG"
                     /locus_tag="PKK95_13535"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOC19289.1"
                     /translation="MSRFYLTTAIDYVNSRPHLGTAYEKITADVIARYQRLLGAETHF
                     LMGNDEHSQNVFRKARELGLDPVAYCDRMEREFREVWGRLDVSFDDFIRTTEERHRVG
                     VTAMVQRIADRGDLYEAFYEGPYCVSCEAFKPEKDLVDGLCPVHLTKPEWIKERNHFF
                     RLSKHRDALLAHFHAHPGFLEPEVRRNEILRLLESGLDDISISRAGQSWGIPLPFDPS
                     SVVYVWFDALINYVSAIGFGGDEASFERWWPANLHVIGKDITRFHCVVWPAMLMSAGL
                     ALPRQVFGHGFVNLKGQKMSKSLGTILDPLEAAERFGPDPLRLYLVKEIAYGQDGDFS
                     WDRFEERYNVDLANNLGNLVNRLAVMVDKYRGGRLPAAGAPGRLAGV"
     gene            complement(1193..2497)
                     /gene="larC"
                     /locus_tag="PKK95_13540"
     CDS             complement(1193..2497)
                     /gene="larC"
                     /locus_tag="PKK95_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOC19290.1"
                     /translation="MARVLYFDCFAGASGDMVLGALLDAGLPLAALEQALGALMLPGT
                     RLSAEKVLRAGVSATKFRLLEEGHGSPGAGPAHSHSHGDDHGHSHPHDHDAEPSHGHG
                     HAHAHEGAGSRAHAHRSVAEINRLIEGSSLSARAKARTIELFGRLAAAEAAIHRMPIE
                     EVHLHEVGELDSIVDVAGAVFGLEWFAIDRFVASPLNVGGGTVRSAHGVFPVPAPATL
                     GLLTGVPVYSSGVQMETVTPTGALLVAGHATEYGAFPAIAVERVGYGAGDRDLPGSPN
                     VLRVVIGDDGAQGPVERVVVMECEIDDMNPQIFGALMNRLYAAGALEVFYTGVQMKKN
                     RPGTLLTVVAPPSRREALAELVFRETTTIGLRYQEVARSCLARETVSVETAWGPIRVK
                     VARLGQEVVNASPEFDDCEKAAARAGVPVKQVQAAALRAYGR"
     gene            complement(2510..3178)
                     /gene="radC"
                     /locus_tag="PKK95_13545"
     CDS             complement(2510..3178)
                     /gene="radC"
                     /locus_tag="PKK95_13545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOC19291.1"
                     /translation="MKALGQADRPREKLARLGASGLGDNELLAALIGTGTVGADALGL
                     ANQLLSACGGLHGVQRASREELLRVRGMGEARAAQVMAAIELGRRALARRAGDRFQVL
                     GPRAAADYLMPEYGGRPIEQFGALLLDTKHRVLRCALLSTGSQDATSVQPREVFRQAL
                     LASATAVLLFHNHPSGDPQPSPEDVELTVRLHAAGELMGVTVVDHVILGDGKYYSLKE
                     SGLW"
     gene            3215..3811
                     /locus_tag="PKK95_13550"
     CDS             3215..3811
                     /locus_tag="PKK95_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HOC19292.1"
                     /translation="MLRLVVRLVVPLVAALALSACAAPPEAEMTEAREALAAARAAGA
                     ERYAPAEYGEAAGMLQRSERFVAEGDYRQALSAALEARELANAAAAAAGGAKEAARAR
                     ATTAMDQAQASLAAARAFTDAATAKPPRTRPERLHVSEVRRAVVAANVAVQKAREAIG
                     RDDFAAATEALDAVRAQLDEVMAAKPEPPPPPKPRRRR"
     gene            complement(4041..>4744)
                     /gene="dapF"
                     /locus_tag="PKK95_13555"
     CDS             complement(4041..>4744)
                     /gene="dapF"
                     /locus_tag="PKK95_13555"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOC19293.1"
                     /translation="ARTLCARHTGVGADGLILYRPTPRGAAMRLFNADGSYSEVSGNG
                     VRALGAVLLHGRADAGNLALVVDTDAGEKRLDLLRRDGTRLTFRAAMGMPEAIVERTL
                     EVGGEALRVVTLRVGNPQCVAVEGALSLERMHRLGALLEHHEAFPEGTNVEFATVESP
                     SRVRILIWERGVGPTLASGTGACASAVAAAVAGGASRDVEVAAPGGTQRVEWTGQGLF
                     LTGWAEVLCRGTWLA"
BASE COUNT          704 a         1762 c         1625 g          653 t
ORIGIN      
        1 caacccccgc gagccgtccg ggcgctcccg ccgccggcag cctgccgccc cggtacttgt
       61 ccaccatcac ggcgaggcgg ttcaccaggt tccccaggtt attcgccagg tcgacgttgt
      121 accgctcctc gaaccggtcc cacgagaagt cgccgtcctg cccgtacgcg atctccttga
      181 ccaggtacag ccgaagcggg tcgggtccga agcgctcggc cgcctcgagc gggtcgagga
      241 tcgtgccgag cgatttactc atcttctgcc ctttgaggtt gacgaagccg tggccgaaga
      301 cctgcctcgg gagcgccagc cccgcgctca tcagcatcgc gggccagacg acgcagtgga
      361 accgagtgat gtccttcccg atgacgtgca ggttcgccgg ccaccaccgc tcgaacgacg
      421 cctcgtcgcc cccgaacccg atcgccgaca cgtagttgat cagcgcgtcg aaccagacgt
      481 agacaacgct cgaggggtcg aagggcagcg ggatgcccca ggactggccg gcccggctga
      541 tggagatgtc gtcgaggccc gactcgagca gccggaggat ctcgttgcgg cgcacctcgg
      601 gctcgaggaa accggggtgc gcgtggaagt gtgcgagcag cgcgtcccgg tgcttcgaca
      661 ggcggaagaa gtggttgcgc tccttgatcc actcgggctt ggtgaggtgc acggggcaga
      721 ggccgtccac caggtccttc tcgggcttga acgcctcgca cgacacgcag tacggcccct
      781 cgtagaacgc ctcgtagaga tccccgcggt cggcaatccg ctgcaccatc gcggtcacgc
      841 cgacgcggtg gcgctcctcg gtcgtccgga tgaagtcgtc gaacgacacg tcgagccgcc
      901 cccacacctc ccggaactca cgttccatgc ggtcgcagta cgccaccggg tcgagcccca
      961 gctcgcgggc cttccggaac acgttctgcg agtgctcgtc gttgcccatc aggaaatggg
     1021 tctcggcgcc gagcagccgc tggtagcggg cgatgacgtc ggccgtgatc ttctcgtagg
     1081 cggtgcccag gtgcggccgg ctgttgacgt agtcgatcgc ggtagtgagg tagaagcggg
     1141 acatagggcg ttcgattgta ccttcgaccc ttctcgttcc gtgcggagcg cgtcaccggc
     1201 catacgcgcg gagcgcggcc gcctgcacct gcttgacggg aaccccggcc cgcgcggctg
     1261 ccttctcgca gtcgtcgaac tcgggcgacg cgttgacgac ctcctggccc agcctcgcga
     1321 ccttgacccg gatggggccc cacgccgtct cgacactcac cgtctcgcgc gccaggcagc
     1381 tgcgcgcaac ctcctggtag cgtagcccaa tggtggtcgt ttcacgaaag acgagctcgg
     1441 ccagcgcctc gcggcgcgag ggcggcgcca ccacggtcag cagcgtcccc ggccggttct
     1501 tcttcatctg cacgccggtg tagaagacct cgagcgcgcc ggcggcgtac aaccggttca
     1561 tcagcgcacc gaagatctgc gggttcatgt cgtcgatctc gcactccatc acgacgaccc
     1621 gctcgacggg cccctgcgcc ccgtcgtcgc cgatgaccac gcgcaggacg ttcggcgaac
     1681 ccggcaggtc gcggtcgccc gcgccgtacc ccacacgctc gaccgcgatc gcgggaaacg
     1741 cgccgtactc ggtggcgtgc cccgccacca gcagcgcgcc cgtcggcgtc accgtctcca
     1801 tctggacgcc gctcgagtag accggcacgc cggtcagcag gcccagcgtc gcgggggccg
     1861 gcacggggaa gacgccgtgg gccgagcgga ccgtcccgcc gccgacgttc aggggcgatg
     1921 ccacgaagcg gtcgatggcg aaccactcga gcccgaacac ggcgccagcc acgtcgacga
     1981 tcgagtcgag ctcgcccacc tcgtgcaggt gcacctcctc gatgggcatc cggtggatcg
     2041 cggcctcggc cgcggccagc cggccgaaca gctcgatggt gcgcgccttg gccctggccg
     2101 acagcgatga gccttcgatc aggcggttga tctccgcgac cgaacggtgg gcgtgggcgc
     2161 gagaccccgc gccctcgtgc gcgtgcgcgt ggccgtggcc gtgagagggc tcggcatcgt
     2221 ggtcgtgcgg gtgagagtgg ccgtggtcgt ccccgtgcga gtgggaatgc gccggcccgg
     2281 cgccgggcga cccgtgcccc tcctcgagaa ggcggaactt ggtcgccgag acgcccgccc
     2341 ggagcacctt ctcggccgac aggcgcgtgc ccgggagcat gagggcgccg agggcctgct
     2401 cgagcgcggc gagtggaagg cccgcgtcga ggagcgcacc cagcaccatg tcacccgaag
     2461 cccccgcgaa gcagtcgaag tagagtacgc gcgccatgtg cctcctgcct caccacagcc
     2521 ccgactcctt gaggctgtag tacttcccgt cgccaaggat gacgtgatcg acgaccgtca
     2581 cccccatcag ctcgccggcc gcgtgcaggc gcacggtcag ttccacgtcc tcggggctcg
     2641 gctgggggtc gccggagggg tggttgtgga acaacagcac cgcggtggcc gaggcgagca
     2701 gcgcctgccg gaacacctcg cgcggctgca cgctggtcgc gtcctgcgat ccggtggaca
     2761 gcagggcgca gcggagcacg cggtgcttgg tgtcgagcag cagcgccccg aactgctcga
     2821 tgggccgccc gccgtactcg ggcatcaggt agtcggccgc cgcccgcggg ccgagcacct
     2881 ggaagcgatc gccggcgcgg cgcgcgagag cccggcgccc cagctcgatg gcggccatca
     2941 cctgcgcggc ccgggcctcc cccatcccgc gcacgcgcag cagctcctcg cggctggccc
     3001 gctgcacgcc gtgcaggccc ccgcacgcgg aaagcagctg gttggccagg ccgagcgcgt
     3061 cggcccccac cgtaccagta ccgatcaggg cggccagcag ctcgttgtcg ccaaggcccg
     3121 aggccccgag acgcgccagc ttctcgcgcg gccggtcggc ttggcccagc gccttcacgg
     3181 cccgctcccc gaatcatcat ttatactgca tcccatgctt cgcctcgtcg ttcgcctcgt
     3241 ggtccccctg gttgcggccc tcgccctctc ggcctgcgcc gcaccgcccg aggccgagat
     3301 gaccgaggca cgcgaggccc tggcggcggc gcgcgctgcg ggggcggagc ggtacgctcc
     3361 cgccgagtac ggcgaagcgg caggcatgct gcagcgctcc gagcggttcg tggccgaggg
     3421 cgactatcgc caggcgctga gcgctgccct cgaggcgcgc gaactggcca acgcggcggc
     3481 ggccgccgcc ggcggcgcga aggaggcggc gcgagcccgt gcgacaaccg cgatggatca
     3541 ggcgcaggca tcgctggccg ccgcgcgtgc cttcaccgac gcggccaccg cgaagccgcc
     3601 gcgcacccgc cccgagcggc tgcacgtctc cgaggtgcgc cgcgcggtgg tggccgccaa
     3661 tgttgccgtg caaaaagcgc gcgaggcgat tggccgcgac gacttcgccg cggccaccga
     3721 ggcgctcgac gccgtgcggg cgcagctcga cgaggtgatg gcggcaaaac ccgagcctcc
     3781 tcccccgccg aagccacgac gccggcgctg accgctctgc gccgaggttg caggaactgc
     3841 tgcgagatcg acggcacggt acgagccgac tgctatccta ttcgccgcac gatcaggcaa
     3901 ccacaaagaa cacaaagaac acaaagaacg acgagcgtgg ggtgctcggg cagcccgaac
     3961 tgcacgagcg ccggcctgcc ccgatgggca cgacttcgcg tcctttgtgt cctttgtggt
     4021 tttctatcgt ggacccgccg tcaggccagc caggtgccgc ggcacaggac ctcggcccag
     4081 cccgtgagga agagcccctg cccggtccac tcgacgcgct gggtgccgcc gggcgccgcc
     4141 acctccacgt cgcgcgacgc gccgccggca acggcggcgg caaccgccga cgcgcaggcg
     4201 ccggtgcccg acgcgagcgt cggccccacg ccgcgctccc agatcaggat ccgcacgcgc
     4261 gagggcgatt ccaccgtggc gaactcgacg ttggtgccct ccggaaaagc ctcgtggtgc
     4321 tcgagcagcg ccccaaggcg gtgcatccgc tccaggctca gcgcgccttc gacggcgacg
     4381 cactgcgggt tcccgacccg cagggtgacc acccgcagcg cctctccccc gacctccagc
     4441 gtacgctcga cgatcgcctc gggcatcccc atcgccgccc ggaacgtcag gcgggttccg
     4501 tcccgtcgca gcaggtcgag ccgtttctcg cccgcatcag tgtcgacgac gagcgcgagg
     4561 ttgccggcgt ccgcacggcc gtgcagcagc acggcgccca gcgcccgcac cccgttgccc
     4621 gacacctccg agtagctgcc gtcggcgttg aacaggcgca tggcggcgcc gcgcggcgtc
     4681 gggcggtaga ggatcaggcc gtcggcgccc acgccggtgt gtcgcgcgca cagggtgcgc
     4741 gcca
//
LOCUS       DAOPQX010000293         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19788_length_4710_cov_4.371858, whole genome shotgun sequence.
ACCESSION   DAOPQX010000293 DAOPQX010000000
VERSION     DAOPQX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19788_length_4710_cov_4.371858"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PKK95_13560"
     CDS             <1..691
                     /locus_tag="PKK95_13560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC19294.1"
                     /translation="PLLALPALLLLARILRGVPEVRLSRGDERNGFGALEPYASPLAL
                     LWTVVVIRTVVAIGFATFLPVFMVGNGLTVSEAGFAVTCYLLAGSFGGFFGGPVADRF
                     GPRLVISWSLLLAVPLLASAAWLSGPLSYVLLTAGGFFLGSTLPVNITYAHMIAPVAT
                     GTVSSLMMGVAWGVGGMAVPLVGLAGDRVGLGPALLVLAFLPAVAALITLALPPGGSH
                     VEAAEGAAASD"
     gene            725..1642
                     /locus_tag="PKK95_13565"
     CDS             725..1642
                     /locus_tag="PKK95_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOC19295.1"
                     /translation="MNRRDFLAAAAAAAAAPVAPALGASGRQGAPGRAANAFHLHFAP
                     HFGQFKAHAGDDLTAQIDFVAGQGFTAMFDNGLMGRPPAQQELIARELSRRNMQLGPF
                     VLYADFKTKSFALRDKDVRAMLLQKMRDGVETMKRTGAKQALVVLGRYDESLAWDYQT
                     ANAVDHLRACMEIVQPAGLVIVLEPLNPRDHPGLFLTRIPQAYQICKAVANPSCKIID
                     DLYHQQITEGNLIPNIEAAWDEIAAFHLGDNPGRKEPTTGEINYRNVFRYIRDRGYQG
                     VLGMEHGQSKPGIEGERAVIEAYRACDPS"
     gene            1660..2940
                     /locus_tag="PKK95_13570"
     CDS             1660..2940
                     /locus_tag="PKK95_13570"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC19296.1"
                     /translation="MSENQTTVTRRDFVKAASAATAAAAFAQPLGVFAQGSDAIKVGV
                     IGCGGRGTGAALDCLKGAQGVEIVALGDLVPDRVESALARLTKEFPDRVKVTKDHQFT
                     GFDNYRGVLSVPGVNLIITAAPPGFRPIHLKAAVEAGKHVFMEKPVAVDPVGVRSVIA
                     SSELAKQKGLAIVAGTQRRHQQRYLEMMKRVQDGQIGEIVAAQCYWNQGDLWVKERTA
                     GMSEIEWQCRNWLYFTWTSGDHIVEQHVHNIDVVNWAMGCMPRNVMGMGGRQVRVAPE
                     YGNIYDHFAVEFEYPNGVRVSSMCRQTKGANDRVEERIVGTKGVALSTSNSTKITGAT
                     EWTFQGDEINPYVQEHIDLIASIRAGKPLNEGRQIAESTMCAIMGRMSAYTGRALSWE
                     WAMNTSKLDLSPAKYEFGPNPVDPVAMPGVTPLV"
     gene            3139..4107
                     /locus_tag="PKK95_13575"
     CDS             3139..4107
                     /locus_tag="PKK95_13575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19297.1"
                     /translation="MTRQLNVSSLAGLCLLAATLAAAQQPPTPPSAAPAVAQEPAADL
                     LKQGQQLLREGKHQEALDAFRDAIAKAPASSPTWASAQLQAGVVLDLLGKYEEARTHL
                     SKAIDAAPPQDKPRARRTMAMSYAFERNCDGAEKYQAPVYESYLEAKDFFMAGEVANE
                     LARVCLESGNSDKALTWYQRGYAAGIKEPGITPERRDLWEFRWEHAQARIAARRGEAD
                     EAKKHVAAAKAIHDKGANPEQAQFVPYLVGYVAFYTGDYQTALAELQKGNQNDPFILA
                     LIAQAHEKLGNKDQAMEYYRKAAASNAHNPTNAYARPLAKEKLGMK"
     gene            complement(4111..4602)
                     /locus_tag="PKK95_13580"
     CDS             complement(4111..4602)
                     /locus_tag="PKK95_13580"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HOC19298.1"
                     /translation="MPPMLSALLWLLAIVLVVVGIAGTVLPALPGPILVLAGLVVGAW
                     ADGFTRVGWLTLGLLAVLAGLSYLVDLAAAALGVRRFGASKRAAVGAALGTMAGLFFG
                     LPGLIVGPFAGAVLGEVWARRGLEGAGRAGAGAWIGFLLGALAKLALVFMMIGLFALA
                     LYW"
BASE COUNT          794 a         1641 c         1646 g          629 t
ORIGIN      
        1 gccgctgctg gccctgccgg cgctgctgct gctggcccgc atcctgcggg gcgtgcccga
       61 ggttcgcctc tcgcgcggcg acgagcggaa tggcttcggg gcgctcgagc cctacgcgtc
      121 gccgctcgcg ctgctctgga cggtggtggt catccggacg gtcgtggcca tcggcttcgc
      181 gacattcctc cccgtgttca tggtcgggaa cggcctcacg gtgagcgagg cggggttcgc
      241 ggtcacctgc tacctgctgg ccgggagctt cggcggcttc ttcggggggc cggtggccga
      301 ccgcttcggc ccgcgcctgg tcatctcctg gtcgctgctg ctcgcggtgc cgctgctcgc
      361 ctcggcggcc tggctgagcg ggccgctgtc gtacgtcctg ctgactgcgg gcggcttctt
      421 cctgggctcg acgctgccgg tgaacatcac ctacgctcac atgattgccc ccgtggccac
      481 cggcaccgtg tcgtcgctga tgatgggcgt ggcctgggga gtgggcggca tggccgtgcc
      541 gctggtgggg ctggcgggcg accgggtcgg cctcggcccg gcgctgctgg tgctcgcgtt
      601 ccttcccgcg gtggccgccc tcatcacgct ggcgctccct ccgggcgggt cgcacgtcga
      661 ggccgcggag ggcgcggccg cctccgattg acacaccgtc gcgcccccaa taggattcgc
      721 agtcgtgaac cgaagagact ttctcgccgc ggcggctgca gccgctgccg cgcccgttgc
      781 ccccgcgctg ggagcatcgg gccggcaggg cgcaccggga cgcgcggcca acgccttcca
      841 cctgcacttc gccccgcact tcgggcagtt caaagcgcac gcgggtgacg acctcacggc
      901 ccagatcgac ttcgtggcgg gccaggggtt caccgcgatg ttcgacaacg ggctcatggg
      961 gcggccgccg gcgcagcagg agctgatcgc gcgcgagctg tcgcgccgca acatgcagct
     1021 cggccccttc gtgctctacg ccgacttcaa gacgaagagc ttcgcgctcc gcgacaagga
     1081 cgtccgcgcg atgctgctgc agaagatgcg cgacggggtc gagacgatga agcggaccgg
     1141 cgcgaagcag gcgctggtgg tgctcggccg gtacgacgag agcctcgcct gggactacca
     1201 gacggcgaac gcggtcgatc acctgagggc ctgcatggag atcgtccagc cggccggcct
     1261 ggtgatcgtc ctcgagcctc tcaatcccag ggatcacccc ggcctgttcc tgacgcggat
     1321 cccgcaggcc taccagatct gcaaggccgt ggcgaacccg tcgtgcaaga tcatcgacga
     1381 cctgtatcac cagcagatca ccgaggggaa cctcatcccc aacatcgagg cggcgtggga
     1441 cgagatcgcg gcgttccacc tcggcgacaa ccccggccgg aaggagccca ccaccggcga
     1501 gatcaactac cggaacgtgt tccgctacat ccgcgacagg ggctaccagg gcgtgctcgg
     1561 catggaacac ggccagagca agccgggcat cgagggagag cgggcggtca tcgaggccta
     1621 ccgcgcgtgc gatccgtcct gatcggagag aaaggcatca tgagcgagaa ccagacgaca
     1681 gtcaccaggc gcgatttcgt gaaggccgcc agcgcggcca cggcggcggc cgcctttgcc
     1741 cagccgctcg gcgtgttcgc ccagggctcg gacgcgatca aggttggcgt gattggctgc
     1801 ggcggccggg gcaccggcgc ggcgctcgac tgcctcaagg gagcgcaggg tgtcgagatc
     1861 gtcgccctcg gcgacctcgt tcccgaccgg gtcgagagcg cgctcgcgcg cctcaccaag
     1921 gagttccccg atcgcgtcaa ggtgaccaag gaccaccagt tcaccggctt cgacaactac
     1981 aggggcgtcc tgtcggtgcc cggcgtgaac ctcatcatca ccgcggcgcc ccccgggttc
     2041 cggccgatcc acctgaaggc ggccgtcgag gcgggcaagc acgtgttcat ggagaagccg
     2101 gtcgccgtcg acccggtcgg cgtgcgctcg gtcatcgctt cctcggagct ggcgaagcag
     2161 aaggggttgg cgatcgtcgc cggcacccag cgccgccacc agcagcgcta cctcgagatg
     2221 atgaagcgcg tccaggacgg ccagatcggc gagatcgtcg ccgcgcagtg ctactggaac
     2281 cagggcgacc tctgggtgaa ggagcgcacg gcggggatgt cggagatcga gtggcagtgc
     2341 cgcaactggc tgtacttcac ctggacctcc ggcgaccaca tcgtcgagca gcacgtgcac
     2401 aacatcgacg tggtgaactg ggcgatgggc tgcatgccgc gcaacgtgat ggggatgggc
     2461 ggccggcagg tgcgcgtcgc gcccgagtac ggcaacatct acgaccactt cgccgtggag
     2521 ttcgagtacc cgaacggcgt gcgggtgagc agcatgtgcc ggcagaccaa gggcgccaac
     2581 gaccgggtcg aggagcggat cgtcgggacc aagggggtcg cgctgagcac gtccaactcc
     2641 acgaagatca ccggcgcaac cgagtggacc ttccaggggg acgagatcaa cccgtacgtg
     2701 caggagcaca tcgacctcat cgcctcgatc cgcgccggca agccgctcaa cgaggggcgc
     2761 cagatcgccg agagcacgat gtgcgcgatc atggggcgca tgagcgccta cacgggccgc
     2821 gcgctgtcgt gggagtgggc gatgaacacg tcgaagctcg acctctcgcc cgccaagtac
     2881 gagttcggcc ccaacccggt tgacccggtg gccatgccgg gcgtgacgcc gctggtgtag
     2941 cagcagagga cagcggacgg ccgatcgcag gcagcgacgg ggaacggagg tgcggtgcag
     3001 gttccgcacg ctccgttctc cgcttcccgt tctccattct ttctccgctc tcactcctcg
     3061 aaatccggcc catctgcccc gcggcaggac cgcgaacgcc ttgcccaaca cgtgtgagga
     3121 gcggggtaga atggacgaat gacccgacaa ctgaacgtca gctcgctggc gggcctgtgt
     3181 ctcctcgcgg caacgctcgc cgccgcacag cagcccccca ccccgccgtc cgcggcgccg
     3241 gccgttgccc aggaaccggc cgccgacctc ctcaagcagg gccagcagct gctgcgcgag
     3301 gggaagcacc aggaagcgct tgacgccttc cgcgacgcga tcgcgaaggc gcccgcctcg
     3361 tcgcccacgt gggcctcagc ccagctgcag gccggcgtcg tgctcgacct gctcggcaag
     3421 tacgaggagg cgcggaccca cctctccaag gcgatcgacg ccgcaccgcc ccaggacaag
     3481 ccgcgcgcgc gccggaccat ggcgatgtcg tacgccttcg agcgcaactg cgacggcgcc
     3541 gagaagtacc aggcgccggt ctacgagtcg tacctggagg ccaaggactt cttcatggcc
     3601 ggcgaagtgg cgaacgagct ggcgcgcgtg tgcctcgagt cgggcaactc cgacaaggca
     3661 ctgacgtggt accaacgcgg ctacgccgcc ggcatcaagg agcctggcat cacccccgaa
     3721 cggcgcgacc tctgggagtt ccggtgggag cacgcgcagg ctcgcatcgc ggcgcgccgc
     3781 ggcgaggcag acgaggcgaa gaagcacgtc gccgccgcca aggcaatcca cgacaagggg
     3841 gccaaccccg agcaggcgca gttcgtgccc tacctggtgg gctacgtcgc cttctacacc
     3901 ggcgactacc agaccgccct cgccgagctg cagaagggga accagaacga ccccttcatc
     3961 ctcgccctga tcgcccaggc gcacgagaag ctggggaaca aggaccaggc gatggagtac
     4021 taccgcaagg cggccgcctc gaacgcgcac aaccccacca acgcctacgc gcgcccgttg
     4081 gccaaggaga agctcggtat gaagtagggc ctaccagtag agcgccaggg cgaagaggcc
     4141 gatcatcatg aagacgaggg cgagctttgc gagggcgccg agcaggaacc cgatccaggc
     4201 tccggcgccc gcgcgccccg cgccctcgag gccgcgccgc gcccacacct cgcccagcac
     4261 cgcgccggcg aacggcccga caatcaggcc cgggaggccg aagaacagcc ccgccatcgt
     4321 gccgagggcg gcgccgacgg ccgcccgctt cgacgcgccg aaccggcgca cgccgagcgc
     4381 ggcggccgcg aggtccacga ggtaggagag ccccgccagc accgcgagca gcccgagcgt
     4441 cagccagccc acccgcgtga agccgtcggc ccaggccccc acgacgaggc ccgcgagcac
     4501 gaggatgggc cccgggaggg cgggcagcac cgtgcccgcg atcccgacca ccacgagcac
     4561 gatcgccaga agccagagca gggcggagag catgggaggc atcacggagg attgtacggg
     4621 aggtctgtcg tgcagaaggt gcagaaggtg caggaggtgc aggaggtgct ggtgccaagg
     4681 gtgcaggagg tgctggtgcc aagggtgcag
//
LOCUS       DAOPQX010000294         4704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19819_length_4704_cov_3.750054, whole genome shotgun sequence.
ACCESSION   DAOPQX010000294 DAOPQX010000000
VERSION     DAOPQX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19819_length_4704_cov_3.750054"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>413)
                     /locus_tag="PKK95_13585"
     CDS             complement(<1..>413)
                     /locus_tag="PKK95_13585"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HOC19299.1"
                     /translation="GRGAAGEVDETPKPGDPRLGGVRTAVIGSGRDAADGAAREAAAR
                     GYRVVMLPEPLVGDAREAGARLVAEGLRAAKGLTPPVSVIAFGETTVHVVGDGRGGRN
                     QELALGAAMALDRDGIEAAVLSGGTDGVDGPTDAA"
     assembly_gap    414..423
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<424..1089)
                     /locus_tag="PKK95_13590"
     CDS             complement(<424..1089)
                     /locus_tag="PKK95_13590"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate-2-kinase family protein"
                     /protein_id="HOC19300.1"
                     /translation="MPALFRAALASVDPARLLRDAFLPGRALAPDPAKPLRLLSVGKA
                     APWLAEACVALQPGRVRAGLVIGTHCPMALPAGLVWHAASHPVPDERSTRAAEAALEL
                     VRGLQADEELVVLISGGASALLALPRPEITLDDKRQTTRTLLGAGADITALNTVRKHL
                     SLVKGGQLGVAAGRGHALAVSDVVGDDLGVIASGPTVPDPTTFADALGVLARYGGPSA
                     YPAR"
     gene            complement(1120..1410)
                     /locus_tag="PKK95_13595"
     CDS             complement(1120..1410)
                     /locus_tag="PKK95_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19301.1"
                     /translation="MSRIGRTLVFPQAPRGRFPIDFYRDELLKHQRCLQRQREYYSEV
                     AITSAEAGVRRLMDRLDQVCRRKDADQVMCRLLRQLDVVTGLSAWSDAKKAN"
     gene            complement(1535..2428)
                     /locus_tag="PKK95_13600"
     CDS             complement(1535..2428)
                     /locus_tag="PKK95_13600"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC19302.1"
                     /translation="MLFAGLKYFVSEAVTGLWRGRRSTVLSVVTIAAALFVLGIFLAV
                     TANLDALVARWGAAAELSVYLRDDVSLAERAEVQRALRGDPLVREVQLVTRDEALARF
                     RRDFPDLAPAVSGPEAHPFPASFEARLAPEASGGDLVESLAVRLRGMRGVSDVRYDRL
                     WLQRLQSIAALVGWGGALVGAVLALAAALTVTNVVRLALFSRRDEIQIMELVGAPLSF
                     IKGPFVMEGILQGALGAILALGALGLAISLARGRLAAAAPGLIDANSIVGLPLATLVL
                     VVVGGMAVGCLGALLAARRLG"
     gene            complement(2438..3094)
                     /gene="ftsE"
                     /locus_tag="PKK95_13605"
     CDS             complement(2438..3094)
                     /gene="ftsE"
                     /locus_tag="PKK95_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOC19303.1"
                     /translation="MIEIEGLSKLYGRDVFALEDFSMHVGKGEFVFLTGPSGAGKSTL
                     LRILLCQEAPTTGRVAVGGRDITRLKPREVQTYRRTLGFVFQDFKLIPGKTVFENVMF
                     ALRILGVPDVQQRRRTFQVLKWVGLQHRANAYPLELSGGEQQRVSIARALVNEPTLVL
                     ADEPTGNLDPDLALEIMNLFREINSTGTTVLVATHDRELIRHVGHRAVTLDHGRLAIG
                     "
     assembly_gap    3156..3255
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3587..>4704)
                     /locus_tag="PKK95_13610"
     CDS             complement(3587..>4704)
                     /locus_tag="PKK95_13610"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOC19304.1"
                     /translation="NVPLPSVPAPAASTPTGQWLARAEGPGAVLYLPLDHDAGNTPAM
                     LQSLEHRRPIVNGYSGQRPSFFMGLVDVLNKLPSAEALWALRDLGVRFVVSPGPLEGS
                     GEPLRERARVPGAVIYELHWTPESEAAVPRPDPPPPPPPGPLPFTASERASFEVTWLS
                     AASLGLPAGEATITAARLDAGTGSPRVAGAALHLAIELRTAPWVSRFFEAHDRLETWV
                     NADLLPLRHEQHLREGRRVVDRTTVFDHAARTLRTGDGPPIGLPTGARDGLAAFLYAR
                     TQPLRPGFSADLPVVEGGRRYAVALAAHAVETIEVRGRRAEALKLTPRMSAVGGRQKN
                     LSVTIYTTTDARRVPLLILVDAGFGSFRLELTDYDAR"
BASE COUNT          692 a         1667 c         1662 g          573 t
ORIGIN      
        1 agcggcgtcg gtcgggccgt ccacgccgtc ggtgcccccg ctgagcaccg cggcctcgat
       61 cccgtcccgg tcgagggcca tcgcggcgcc gagcgccagc tcctggttcc gcccgccgcg
      121 cccgtcgcca accacgtgca cggtcgtctc gccgaaggcg atcaccgaca ccggcggggt
      181 cagtcccttc gcagcccgca gcccttcggc caccagccgc gcccccgcct cgcgggcgtc
      241 gccgacgagc ggctcgggta gcatcaccac ccggtacccg cgagccgccg cctctcgtgc
      301 cgcgccgtcg gccgcgtcgc gccccgaccc gatcacggcg gtcctgaccc cgcccaggcg
      361 cgggtctccg ggcttcggcg tctcgtccac ctcgcccgcc gcgccccgac cgannnnnnn
      421 nnngcgcgcg gggtaggcgg agggcccccc gtaacgagcg agcacgccga gcgcgtcggc
      481 gaaggtcgtg ggatcgggca cggttgggcc cgaggcaatc acgccgaggt cgtcgccgac
      541 cacgtccgag acggccaggg cgtggccgcg cccggcggcg acaccgagct gtccaccctt
      601 gacgagtgaa aggtgcttcc ggaccgtgtt gagggccgtg atgtcggcgc cggcaccgag
      661 cagcgtgcgc gtcgtctgcc tcttgtcgtc gagcgtgatc tcgggccgcg gcagcgcgag
      721 cagcgccgag gcccctcccg agatcagcac gacgagctcc tcgtcggcct ggaggccgcg
      781 caccagctcg agtgccgcct cggccgcccg tgtgctgcgc tcgtccggca ccgggtggct
      841 ggccgcgtgc cacacaagcc cggccgggag cgccatcggg cagtgcgtgc cgatcacgag
      901 gcccgcccgg acgcgtcccg gctgcagcgc gacgcacgcc tccgccagcc acggcgcggc
      961 cttgccgacc gagagcagcc ggagcggttt cgccgggtcg ggcgcgagcg cgcgcccggg
     1021 caggaaggcg tctcgaagca gccgcgcggg atcgaccgac gcgagcgccg cccggaagag
     1081 ggcgggcagg tcgcggcgga gcggcgatgg gagggtcagt taattggcct tcttcgcgtc
     1141 ggaccacgcc gaaaggccgg tcacgacgtc gagctggcgc aggaggcggc acatcacctg
     1201 gtccgcgtcc ttccgccggc acacctggtc gaggcgatcc atcaggcgcc ggacgccggc
     1261 ctcggcgctg gtgatcgcca cctcggagta gtactcgcgc tgccgctgga ggcaacgctg
     1321 atgcttgagc agttcgtccc ggtagaaatc gatcgggaac cgtccccgcg gcgcctgtgg
     1381 gaacacgagg gtgcgaccga tgcgactcat attcgattca agggaatgac cggcgggcaa
     1441 tctcagtcta ggacgcccgc cgcggggctg tcaacggcga tcgttcgatt gccggcggcc
     1501 ggaatcgctt gtccggcgcg gcccggcggc cgcctcagcc gaggcggcgc gccgccagca
     1561 gcgcgccgag gcacccgacg gccatcccgc ccacgacgac gagcacgagg gtcgcgagcg
     1621 gcagtcccac gatgctgttc gcgtcgatca ggcccggcgc ggcggcggcg agccgcccgc
     1681 gcgcgagcga gatcgcgagc cccagcgcgc cgagcgcgag gatcgcgccg agcgccccct
     1741 gcaggatccc ctccatcacg aaggggcctt tgatgaacga caggggggca ccgaccagct
     1801 ccatgatctg gatctcgtcg cggcgcgaga agagcgccag cctcaccacg ttggtgaccg
     1861 tgagggcggc ggcgagggcg agaaccgcgc caaccagcgc gccgccccat ccgaccagcg
     1921 cggcgatcga ctgcaggcgc tgcagccaca gccggtcgta ccggacgtcg ctgacgcccc
     1981 gcatcccgcg caggcggacg gcgagcgatt cgacgaggtc gccgcccgag gcctcggggg
     2041 cgagtcgcgc ctcgaacgac gcggggaacg ggtgagcttc cgggcccgag accgcgggtg
     2101 ccaggtcggg gaagtcgcgc cggaaccgcg cgagcgcctc gtcgcgcgtc accagctgca
     2161 cctcgcggac gaggggatca ccgcgcagcg cccgctgcac ctccgcccgc tcggccagcg
     2221 agacgtcgtc acggaggtac acggacagct cggcggccgc gccccagcgc gccaccagcg
     2281 cgtccaggtt cgccgtgacg gcgaggaaga tccccagcac gaacagcgcc gcggcgatcg
     2341 tcaccaccga gaggaccgtc gagcgccgcc cccgccacag ccccgtcacc gcctcgctca
     2401 cgaagtactt cagcccggca aagagcatgg gtggacccta cccgattgcc agccgcccgt
     2461 gatccagcgt cacggcgcga tgccccacgt gccggatcaa ctcgcggtcg tgcgtggcga
     2521 ccagcacggt cgtccccgtc gagttgatct cgcggaacag gttcatgatc tcgagcgcga
     2581 gatcgggatc gaggttgccg gtcggctcgt cggcgagcac cagcgtcggc tcgttcacga
     2641 gcgcccgggc gatcgacacg cgctgctgct cgccgcccga gagctcgagc gggtacgcgt
     2701 tcgcccggtg ctggagcccg acccacttca gcacctggaa ggtgcggcgc cgctgctgca
     2761 cgtcgggcac ccccaggatg cggagcgcga acatcacgtt ctcgaagacg gtcttcccgg
     2821 ggatcagctt gaagtcctgg aacacgaagc cgagggtgcg gcggtaggtc tgcacctcgc
     2881 gcggcttgag gcgcgtgatg tcgcgcccgc cgacggcgac gcggccggtg gtcggcgcct
     2941 cctggcacag caggatccgc agcagcgtgc tcttgccggc gccgctcggc cccgtcagga
     3001 agacgaactc gcccttcccg acgtgcatgc tgaagtcctc gagcgcgaac acgtcgcggc
     3061 cgtagagctt cgacaggccc tcgatctcga tcacggcaac tcgtctcgga gaagggcgcc
     3121 cctgcgcgcc gcgtgaagtg cgcgcagtat agcatnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnntcgtc tcggagaagg gcgcccctgc gcgccgcgtg aagtgcgcgc
     3301 agtatagcat tagaaaacag cggacagcta acagctgaca gctaacagct gacagctgac
     3361 agctgacagc cagctcatag ctgacagcta acagcggaca gctaacagct gacagctgac
     3421 agccgacagc cgacggcgga gagcactgcc cgcaaccgga gcgggtggca gggcgggaca
     3481 atgtcgcgaa ccagggcgtt cggcgtcagt cccgattggg attcggattg ggattcggat
     3541 tcggattggg attgggattg ggattgggat tgggattcat agaggttcag cgcgcgtcgt
     3601 agtccgtcag ctcgaggcgg aaggagccaa agccggcgtc gaccaggatg agcagcggca
     3661 cccgcctcgc gtcggtcgtc gtgtagatcg tcacggagag gttcttctgt cgcccgccga
     3721 ccgcgctcat gcggggggtc agcttcagcg cctcggcgcg gcgcccccgc acctcgatcg
     3781 tctccaccgc gtgggcggca agcgccaccg cgtagcgccg gccgccctcc accacgggga
     3841 ggtcggccga gaagccgggc cgaagcggtt gcgtgcgcgc gtagaggaac gcggcgaggc
     3901 cgtcgcgcgc ccccgtcggc agcccaatgg gcgggccgtc accggttcga agcgtccgcg
     3961 ccgcgtggtc gaagacggtg gtccggtcca ccacccgtcg cccctcccgc aggtgctgct
     4021 cgtggcgcag cggcagcagg tcggcgttta cccacgtctc gagacggtcg tgcgcctcga
     4081 agaagcgcga gacccacggc gcggttcgca gctcgatcgc caggtgcagc gccgcgcccg
     4141 cgaccctcgg cgacccggtt ccggcgtcga gccgcgcggc ggtgatcgtc gcctcgccgg
     4201 ccggcaggcc gagggaggcc gcgctcagcc acgtgacctc gaaagacgct cgctccgagg
     4261 cggtgaacgg gagcggcccg ggcggcggcg ggggcggcgg gtcggggcga ggcacggccg
     4321 cctcggactc gggcgtccag tgcagctcgt agatcacggc gccggggacg cgcgcccgct
     4381 cgcgaagcgg ctccccggag ccctcgagcg gccccggcga gacgacgaac cgcacgccga
     4441 ggtcgcggag cgcccacagc gcctcggccg acggcagctt gttcagaacg tccacgagcc
     4501 ccatgaagaa cgacgggcgc tgcccgctgt acccgttgac gatcgggcgg cggtgttcga
     4561 gcgactgcag catggcggga gtgttgcccg catcgtggtc gagcggcagg tagaggacgg
     4621 cgcccgggcc ctcggcgcgg gccagccact gccctgtcgg cgtcgaggcc gccggcgccg
     4681 gtaccgacgg cagcggcacg ttca
//
LOCUS       DAOPQX010000295         4687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19901_length_4687_cov_4.397453, whole genome shotgun sequence.
ACCESSION   DAOPQX010000295 DAOPQX010000000
VERSION     DAOPQX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19901_length_4687_cov_4.397453"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..618)
                     /locus_tag="PKK95_13615"
     CDS             complement(67..618)
                     /locus_tag="PKK95_13615"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HOC19305.1"
                     /translation="MRRNLLAALALSLLAGPAPATAQPSFSPPGLITSAGQGADAAIV
                     KLALNTQYQLGLDYQPAAGIADLAGRKTLIVVVGTTPEALAAAGVDLPHELARVRALL
                     AAAREAGVGVLLVHTGGQARRDKASNQLIELTVPLADYIVVVAAGNADKLFHKLAAAR
                     GTPVVQIDRVTGVGGAVSAVYKR"
     gene            734..1063
                     /locus_tag="PKK95_13620"
     CDS             734..1063
                     /locus_tag="PKK95_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOC19306.1"
                     /translation="MDVQYYRDALVRKRDEILSTGGMKPLQSETNSRQGDLADQASGN
                     NEVHIQLKLKQTDAKILQAIDEALIRIDKGTYGICRDCGEPIARARLEAIPWTRVCKT
                     CKEKQTA"
     gene            1060..1548
                     /locus_tag="PKK95_13625"
     CDS             1060..1548
                     /locus_tag="PKK95_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19307.1"
                     /translation="MKPSELLALLLEFHAEKAGLYGRHVAVARVAAHYDVNNVYQYVI
                     AREEQHLDWLRQAILDAGGDVPGAAGPPEPERPRDEGALRELARADAGAVRAAVEKWR
                     SRLVQITNARHRKMLELTLGEMLEQARFFDQAASGRLDLLGRRTGGPRTPGAVVASRW
                     RA"
     gene            1545..2015
                     /gene="folK"
                     /locus_tag="PKK95_13630"
     CDS             1545..2015
                     /gene="folK"
                     /locus_tag="PKK95_13630"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOC19308.1"
                     /translation="MSRLVRVAVALGSNIGDRRANLAAAAGRLDSLLRGSRTSSFIET
                     DPVGVGSQPKFLNAAMTGLAALDARSLLEALLAIEREGGRERPFPGSPRTIDLDLILY
                     GEVRLDEPGLVVPHPRFRERRFVLEPLAEIAPGMIDPVTGKTVQQLLQQLTAGS"
     gene            complement(2041..2502)
                     /locus_tag="PKK95_13635"
     CDS             complement(2041..2502)
                     /locus_tag="PKK95_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19309.1"
                     /translation="MRRAAFCTAVLALAVTALAFAQAKPDFSGTWTLDPAKSVMGGGP
                     GGPPGGRGPGGPMTVKQTATELARETSRGERTMTMTYKLDGTESTNAMGPMTSKSTAS
                     WNGDQLVIKTVRETPNGTMETTETWSLGAGGKELTIVTASARGERKMVYTK"
     assembly_gap    2581..2680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2681..3353)
                     /locus_tag="PKK95_13640"
     CDS             complement(<2681..3353)
                     /locus_tag="PKK95_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC19310.1"
                     /translation="MSIVMTFRIALKALNRNKMRTMLTMLGMIIGVAAVITMVALGNG
                     ASQSIEQQIASAGTNVIMVNAGNFTSGGVRMGAGASNTLMPEDAEAIRTQVPGVQYVA
                     AGVDQGAQVVAGNQNWFSRVQGTDVELPMIRAWATESGAFFTPSDVSSAAKVCVIGTT
                     VRDMLFPDGSDPVGQMIRIRNQPFKIVGVMASRGQSAMGQDQDDVIFAPYTTIQKKFR
                     GITYIQ"
     gene            complement(3381..4124)
                     /locus_tag="PKK95_13645"
     CDS             complement(3381..4124)
                     /locus_tag="PKK95_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC19311.1"
                     /translation="MPVISVRDLTRVYQVGDIEVRALRGVSLDVEAGEFVAVTGPSGS
                     GKSTFMHILGCLDRPTGGQYLLDGRDVSRLSRNELAAIRNHQIGFVFQGFNLLSRTSA
                     IENVELPLLYNGRPSKTSERHRRAMDALRLVGLEDRSHHYPNQLSGGQQQRVAIARAL
                     INNPSILLADEPTGNLDTRTSIEVMDVFQKLNADRGITVLLITHERDIAEYGTRVVAF
                     RDGHIVTDEKIARRRVAAEEMAALPPVSV"
     gene            complement(4145..>4687)
                     /locus_tag="PKK95_13650"
     CDS             complement(4145..>4687)
                     /locus_tag="PKK95_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19312.1"
                     /translation="AAGSSRPGLAGQPGERGGGDRRFDPNDPERRARMMERLKQMPEA
                     ERQQFLARLKERGIDLDAQPSGAGARPGQARTQEAVPAMATGGATTIDSLFGPLPTTT
                     SVGRVWLWLSGQKQIKGPIRLRLGISDGQNTELLDGDLKEGQELVAAVQIGGSPVQGR
                     GNSGSPLIQQRGGPPGGFRR"
BASE COUNT          642 a         1607 c         1618 g          720 t
ORIGIN      
        1 gaggccgagg ccgagcagca ccccgagcgc ggcgagcagc gggatcagcg gttccaccac
       61 aggctgctag cgcttgtaga cagcgctcac cgctccgccc acgccggtga cccgatcgat
      121 ctggacgacc ggcgtgcccc tggcagcggc gagcttgtgg aacagcttgt ccgcgttccc
      181 cgctgccacc accacgatgt agtcggcgag gggaaccgtc agctcgatca gctggttcga
      241 ggccttgtcc cggcgcgcct gccccccggt gtggacgagc agcacgccca ccccggcctc
      301 gcgcgccgcc gccagcagcg ccctgacccg ggccagctcg tgagggagat cgacgccagc
      361 cgcggcgagc gcctcgggcg tggttccgac cacgacgatg agcgtcttcc ttccagccag
      421 gtcggcgatg ccggcggcgg gctgatagtc gaggccgagc tggtactgcg tgttcagcgc
      481 cagcttgacg atggcggcgt cggccccctg gccggccgag gtgatgaggc ccgggggaga
      541 gaacgacggc tgcgctgtgg cgggtgccgg cccggccagg agggacaggg ccaacgcggc
      601 gaggaggttc ctgcgcatgg gcacaaggat accggatgat cgccgcggcc ggcccgacgt
      661 ccggcgggcc tcccggcggc tcgtccggtc cgtgctatga ttgcagtttc gaggggccac
      721 cgaggagagt ggaatggacg ttcagtacta ccgggacgcg ctggtccgca aacgggacga
      781 gatcctctcg acgggcggga tgaaacccct gcagagcgaa acgaacagcc ggcagggcga
      841 cctcgcggat caggccagcg ggaacaacga ggtccacatc cagctcaagc tgaagcagac
      901 cgacgcgaag atcctgcagg ccatcgacga ggcgctcatc cggatcgaca agggcaccta
      961 cggcatctgc cgcgactgcg gtgagccgat cgcgcgtgcg aggctcgagg cgatcccgtg
     1021 gacgcgcgtc tgcaagacct gcaaggaaaa acagaccgcg tgaagccctc cgagctcctg
     1081 gccctgctgc tcgagttcca cgccgagaag gccgggctgt acggacgcca cgtcgcggtc
     1141 gcgcgggtcg cggcgcacta cgacgtcaac aatgtctatc agtacgtgat tgcgcgcgag
     1201 gagcagcacc tcgactggct tcgccaggcc atcctcgatg ccgggggcga cgttcccggg
     1261 gccgccgggc cgcccgagcc cgaaaggccg cgagacgagg gcgctctccg cgagctggcc
     1321 cgggccgacg ccggcgcggt cagggccgcg gtcgagaagt ggcgctcgcg cctcgtgcag
     1381 atcacaaacg cccggcaccg gaagatgctc gagctgacgc tcggcgagat gctcgagcag
     1441 gcccgcttct tcgaccaggc ggcgtcgggc cgtctcgacc tgctgggccg ccggacgggc
     1501 ggcccgagga caccgggcgc cgtcgtcgct tctcgctggc gcgcatgagc cggctcgtca
     1561 gggtggcggt tgccttgggc agcaacatcg gggaccgtcg cgcgaacctc gccgcggccg
     1621 ccggtcgcct cgactcgctc ctccgcggtt cgcgaacctc ctccttcatc gagaccgacc
     1681 cggtcggggt cgggtcgcag ccgaagttcc tcaatgccgc gatgaccggc ctggccgcgc
     1741 tcgacgcccg ctcgctgctc gaggcgctgc tggcgatcga gcgcgagggc gggcgcgaac
     1801 gcccgttccc cggctcgccc cgcacgatcg acctcgacct catcctctac ggtgaggtcc
     1861 gcctcgacga gccgggcctc gtggttccgc accctcgctt ccgtgagcgg cgcttcgtgc
     1921 tcgagccgct tgccgagatc gctccgggca tgatcgatcc ggtgaccggg aagacggtgc
     1981 agcagctcct gcagcagctg acggcaggca gctgacagct gaccgcgacc gctgaccgcc
     2041 ctacttggta tagaccatct tccgctcgcc gcgcgcgctg gcggtcacga tcgtcagctc
     2101 cttgccgccg gccccgaggc tccaggtctc ggtggtctcc atggttccgt tgggggtctc
     2161 gcgcaccgtc ttgatgacca gctggtcgcc gttccacgag gcagtgctct tcgaggtcat
     2221 cggccccatg gcgttggtgc tctcggtgcc atcgagcttg taggtcatcg tcatcgtccg
     2281 ctcgccgcgg ctggtttcgc gggccagttc ggttgccgtc tgcttgacgg tcatcgggcc
     2341 gcccgggccg cgccctcccg gggggccgcc ggggccgccg cccatgacac tctttgcggg
     2401 gtcgagcgtc caggtgcccg agaagtcggg cttcgcctgg gcgaacgcga gggcggtgac
     2461 agccagggcc agcaccgcgg tgcagaatgc cgctctcctc atcggttctc ctccgtgtct
     2521 gtccttcaca ggcgggacgc ctgtgctact cgtatcgaag cgcctcgatc gggtcgagcg
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tctggatgta ggtgatgccg
     2701 cggaacttct tctggatcgt cgtgtagggg gcgaagatca cgtcgtcctg gtcctggccc
     2761 atggccgact ggccgcggct ggccatcacc ccgacgatct tgaacggctg gttccggatg
     2821 cggatcatct gccccaccgg gtcggagccg tccgggaaga gcatgtcgcg cacggtggtg
     2881 ccgatgacgc agaccttggc ggcgctggag acgtcggacg gcgtgaagaa ggcgccgctc
     2941 tcggtcgccc acgccctgat catcggcagc tcgacgtcgg tgccctgcac gcgcgagaac
     3001 cagttctggt tgccggcgac gacctgcgcc ccctggtcca cccccgccgc gacgtactgg
     3061 actccgggca cctgggtgcg gatggcctcg gcgtcctcgg gcatcagcgt gttcgacgcg
     3121 ccggcgccca tccgcacgcc gcccgaggtg aagttgccgg cgttcaccat gatgacgttg
     3181 gtgccggccg aggcgatctg ctgctcgatc gactgcgacg cgccattgcc gagggccacc
     3241 atcgtgatca ccgccgccac gccgatgatc atccccagca tggtgagcat cgtccgcatc
     3301 ttgttgcggt tcagcgcctt gagcgcgatg cggaaggtca tcacgatcga catgtctgtc
     3361 ctcgccctct cggccgcctg ctagaccgac accggcggca gcgccgccat ctcctccgcg
     3421 gcgacgcggc ggcgggcgat cttctcgtcg gtgacgatgt ggccgtcgcg gaacgcgacg
     3481 acgcgggtgc cgtactccgc gatgtcgcgc tcgtgggtga tcagcagcac cgtgatgccg
     3541 cggtcggcgt tcagtttctg gaagacgtcc atcacctcga tcgacgtgcg ggtgtcgagg
     3601 ttcccggtcg gctcgtcggc gagcaggatc gacgggttgt tgatgagcgc tcgcgcgatc
     3661 gcgacccgct gctgctgccc gcccgagagc tggttgggat agtggtgcga ccgatcctcg
     3721 agccccacga gccgcagcgc gtccatcgcc cgccggtggc gctcggaggt cttcgagggg
     3781 cggccgttgt acagcagggg cagctcgacg ttctcgatcg cggaggtgcg cgacaagagg
     3841 ttgaagccct ggaagacgaa gccgatctgg tggttgcgga tcgccgccag ctcgttgcgg
     3901 ctcaggcgcg agacgtcgcg cccgtcgagc aggtactgcc ccccggtcgg ccggtcgagg
     3961 cagccgagga tgtgcatgaa ggtcgacttg cccgagcccg aggggccggt gacggcgacg
     4021 aactcgccgg cctcgacgtc gaggctcacg ccgcgcaggg cgcggacctc gatgtcgcca
     4081 acctggtaga cccgggtcaa gtcccggaca gagatcactg gcatagccgc tccgcctcgc
     4141 ccgcctaccg ccggaagcct cccggcggcc cgccccgctg ctggatcagg ggagagccgc
     4201 tgttcccccg gccctgcacg ggcgagccgc cgatctggac cgcggcaacc agttcctggc
     4261 cctccttcaa gtctccgtcg agcagctcgg tgttctggcc gtccgagatg cccagccgca
     4321 gccggatcgg gcccttgatc tgcttctggc cgctgagcca gagccacacg cggccgacgc
     4381 tggtcgtggt gggcagcggg ccgaacagcg agtcgatcgt ggtggcgccg ccggtcgcca
     4441 tggcgggcac ggcctcctgg gtgcgcgcct gccccgggcg ggcgcccgcg cccgagggct
     4501 gggcgtcgag gtcgatgccg cgctccttga gcctggccag gaactgctgg cgctcggcct
     4561 cgggcatctg cttcagccgc tccatcattc gtgcacggcg ctcggggtcg ttggggtcga
     4621 agcggcggtc gccgcccccg cgctcgccgg gctgaccggc gagacccggc ctcgagcttc
     4681 cggcggc
//
LOCUS       DAOPQX010000296         4678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_19956_length_4678_cov_4.578845, whole genome shotgun sequence.
ACCESSION   DAOPQX010000296 DAOPQX010000000
VERSION     DAOPQX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19956_length_4678_cov_4.578845"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKK95_13655"
     CDS             <1..781
                     /locus_tag="PKK95_13655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOC19313.1"
                     /translation="LARLADAVRDADVPDRAVAVTFDDGYLDVLTGAVPILVECEVPA
                     TAFVTSEGGQPGYEFWWDLLDRIFAPGNALPSELVLPTAGIHEASLADPPARAALRQR
                     LTEILYALPEVPRKRLERELAEWGGAEAGPERPAILNLEGIARLAATPGITIGAHGAR
                     HLCLPLHDEDVQRRDVEENKRLLERIAGYPVTSFAYPFGERSPATVRIVREAGFTIGV
                     TVDGRAATGGMPRLLLPRCEVPPGDDAGFKAWLWGLFDARD"
     gene            complement(836..1813)
                     /locus_tag="PKK95_13660"
     CDS             complement(836..1813)
                     /locus_tag="PKK95_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19314.1"
                     /translation="MSGAVRCGRCGIRYHSRYEKCPRCRSRDPRPVALGAPRPGGRRG
                     ATRDRADLQRGTKARRYGQAAVAAAAALVLLLTGWVWGPAPSANVSPAAAAAAKEGPL
                     AAMLRATSPRTFVPGEQVPTELPFIDAATAGTLAYSRGELEHALEMFERQIADRPSDA
                     ESHSNAGQVLIRLGRPIDALPLLERAVALDAGRWAYRFNLARCRGLLGDWGGAISDYR
                     AADKLFPGDYATLFNLAQALHRAGRDEEAAAQYREAIEAKPDDASFHLALAISEEKRG
                     EAAAAVAAYREFLAIAPDAVEAKGVSVRVEELERTAAPPPPPAAPGPVS"
     gene            complement(1810..>4678)
                     /locus_tag="PKK95_13665"
     CDS             complement(1810..>4678)
                     /locus_tag="PKK95_13665"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOC19315.1"
                     /translation="QPARRTTCDAPVVVPSGSPLAGIADTGACDGGGLLGIFATSVAG
                     LNSTGLSTSSTLVPPAAGTAQSVLNVLGTDTTNGADTNPLLIPAGLDTQGFEDRPLTQ
                     LLGDVKSAASSAIVNDTACSNCRNTVVVLVTGGRDHHADPSAKAAEFKALAAGGGHKV
                     PIFVVWVRPNPQSPNYAADQVQLQTIADSSGGVLIRAWNRPGVARAVNLALQAGFSRP
                     PDFNAQTERRSSEYFPVSPVVGTVNLEEASDSTATLLVNHTVYTATGDVIPQRNNFML
                     TAGFGLGLADAHAFPDGPGFYGRLRAFRVFKPIPDSTKPSGYTFTKDGTPLWPDLDGR
                     PELANLARVPRNPDSRNIYTYIPTAGMVAFTPENHWLIAPFLGPTNNATPLNTSNVEA
                     RDLIGYVRRQPLGAVIGSTPAIMDPPSLDPPPDDDYGRTDATGTYAGDHKDRRTIIWI
                     GANDGMLHAIDARTGYEVWAFIPYNLLPKLRAMYEQGQPMEQFNYYMDQSPKIAEIKT
                     GGEWRSYLVIGEGPGGTFYQTFDVTEAGMGGPPPDSDDYEGVLASFASPSRVQFKWAF
                     PDYRSFDTSVRQDIWLTSTGTPSGTPTDATPGSVYRVYGELRASATAAEKTVGQTWSD
                     PAVGALNTDRSVNVAMVGSGYYPLGAETVLAGRGGASAGHAFYLIRMDTGKLLGNPGG
                     NGCTGMGCYDVGDGSPSPKNALQADPTVTGNYGSYMARWAYMGDLDGRYYRFEFDATG
                     EIKIPRVIKTTDQPIYASSALMYVGSAYRYIFFMTGSDLLPTSPSSSGGIGEFKLFGV
                     QDVNGVFSDSFDPIEMAPVTDLGGLPVGERPSTSPAVAGEIVFFTTTVDVAPACSTDL
                     AGSPVDTMHNLYALTYQGTGAYLGTGVSSGKKGGGGTASVLFETGAGRATAPFVVDQH
                     LYFATSGAGGSKVEAYGDPNDFNNGVGQVGVRILSWREIRR"
BASE COUNT          623 a         1611 c         1718 g          726 t
ORIGIN      
        1 gctcgcgcgg ctggccgatg ccgttcgcga cgcggacgtg cctgatcgcg cggtcgccgt
       61 caccttcgac gacgggtacc tcgacgtgct gaccggggcc gtgccgatcc tggtcgagtg
      121 cgaggtgcca gccaccgcct tcgtgaccag cgagggcggg cagcccgggt acgagttctg
      181 gtgggatctg ctggatcgaa tcttcgctcc gggcaacgcg cttccctcgg agctggtgct
      241 acccacggcg gggattcacg aggcctccct tgcagacccg cctgccaggg ctgcgctgcg
      301 ccagcgcctg accgagatcc tctacgcgct tccggaggtg ccgcgaaaga ggctggagcg
      361 cgagctggcg gagtggggcg gcgcggaggc gggtcccgaa cggcccgcca ttctgaacct
      421 ggaggggatt gcgcggctgg ctgcaacgcc cggcatcacg atcggtgcgc acggagcgcg
      481 tcatctctgc ctgccgcttc acgacgagga cgtccagcgt cgtgacgtgg aggagaacaa
      541 gcgcctgctc gagaggatcg ccgggtaccc ggtgacctcc ttcgcctacc ccttcggcga
      601 acgcagcccg gcgacggtac gcatcgtccg cgaagccggc ttcacgatcg gcgtcaccgt
      661 cgatggccgc gccgcaaccg gcggcatgcc gcggctgctg ttgccgcggt gcgaggtgcc
      721 gccaggggac gacgccggct tcaaagcctg gctctggggc ctgttcgacg cccgcgactg
      781 atgcccaccg ggcgcacgat cgagctcgcc gtcccgcgcg gggagcgccg gggcctcagc
      841 tgaccggccc cggcgcggcg ggcggcggcg gcggggccgc ggttctctcc agctcctcca
      901 cccgcacgct cactcccttg gcctcgaccg catcgggtgc gatggccagg aactcgcggt
      961 aggcggcaac cgccgccgcg gcctcgccgc gcttctcctc gctgatggcg agggcgaggt
     1021 ggaagctcgc atcgtcgggc ttcgcctcga tcgcctcgcg gtactgcgcc gccgcttcct
     1081 cgtcccggcc cgcgcggtgg agcgcctgcg ccaggttgaa gagggtggcg tagtcgccgg
     1141 ggaacagctt gtcggcggcg cggtagtcgg agatcgcccc gccccagtcg ccgagcaggc
     1201 cgcggcagcg cgccaggttg aagcggtagg cccaccgccc ggcgtccagc gcgaccgccc
     1261 gctcgagcag cggcagcgcg tcgatggggc ggccgagccg aatcaacacc tgccccgcgt
     1321 tgctgtgcga ctcggcatcg ctcgggcgat cggcaatctg ccgctcgaac atctcgaggg
     1381 cgtgctcgag ctctcccctc gagtaggcga gcgtgccggc cgtcgccgcg tcgatgaacg
     1441 gcagctcggt gggcacctgc tcgcccggca cgaaggtccg cggcgaggtg gcccgcagca
     1501 tcgcggccag cgggccctcc ttcgctgctg ccgccgccgc cggcgagacg ttggccgagg
     1561 gggccgggcc ccagacccag ccggtcagca gcaggacgag ggccgcggcc gcggcgacgg
     1621 ccgcctggcc gtaccgacgc gccttggtcc ctctttgaag atcggctcgg tccctcgtgg
     1681 cacctcgccg cccgccgggg cgcggggcgc cgagcgcgac cgggcgggga tcgcggctcc
     1741 ggcaccgcgg gcacttctcg taccgggagt ggtaccggat cccgcaccgc ccgcacctga
     1801 ccgcgccgct catcgccgga tctcccgcca ggagaggatg cgcacgccca cctggccgac
     1861 gccgttgttg aagtcgttcg ggtcgccgta tgcctccacc ttcgagccgc ccgcaccgga
     1921 ggtggcgaag tagaggtgct ggtccacgac gaagggtgcc gtcgcgcgcc ccgcccccgt
     1981 ctcgaacagg acggacgccg tgccgccgcc tcccttcttc ccgctgctca cgccggttcc
     2041 caggtaggcg ccggtgcctt ggtacgtcag cgcgtacagg ttgtgcatcg tgtccaccgg
     2101 cgagcccgcg aggtccgtcg agcacgcagg cgcgacgtcc accgtcgtcg tgaagaacac
     2161 gatctccccc gcgacggcgg gggaggtgga tggccgctcg ccgacgggca gcccccccaa
     2221 atcggtgacc ggcgccatct cgatggggtc gaacgagtcg gagaacacgc cgttgacgtc
     2281 ctgcacgccg aagagcttga actcgccgat tccgcccgac gaggagggcg aggtcgggag
     2341 gagatcgcta cccgtcatga agaagatgta ccggtaggcc gagccgacgt acatcagggc
     2401 ggacgaggcg tagatcggct ggtcggtcgt cttgatcacg cggggaatct tgatctctcc
     2461 cgtggcgtcg aactcgaagc ggtagtacct tccgtcgaga tctcccatgt acgcccacct
     2521 ggccatgtac gagccgtagt tgcccgtcac cgtcgggtcg gcctgcagcg cgttcttggg
     2581 cgacgggctg ccgtcgccca cgtcgtagca ccccatcccg gtgcaaccgt tgccccccgg
     2641 gttgccgagg agcttcccgg tgtccatccg gatgaggtag aaggcgtggc cggcgctggc
     2701 accgccgcgg ccggccagca cggtttcggc accgagcggg tagtacccgg agccgaccat
     2761 cgcaacgttc acgctgcggt cggtgttgag cgcgccaacc gcggggtccg accaggtctg
     2821 gccgacggtc ttctccgccg cagtcgcgct ggccctcagc tcgccgtaga cccggtagac
     2881 gctgccgggc gttgcgtcgg tcggcgtccc cgagggagtg ccggtcgagg tgagccagat
     2941 atcctggcgg acgctcgtgt cgaagctgcg atagtcgggg aaggcccact tgaactggac
     3001 gcggctcggg ctcgcaaacg aggcgagcac cccctcgtag tcgtcgctgt cggggggcgg
     3061 cccgcccatg ccggcctcgg tcacgtcgaa cgtctggtag aaggtgccgc cgggcccctc
     3121 gccgatcacc aggtagctgc gccactcgcc gcccgtcttg atctcggcga tcttcggcga
     3181 ctggtccatg tagtagttga actgctccat gggctgcccc tgctcgtaca tcgcgcggag
     3241 cttcggcagg aggttgtagg ggatgaaggc ccacacctcg tagccggtcc gtgcgtcgat
     3301 cgcgtggagc atgccgtcgt tggcgccgat ccagatgatc gttcgacggt ccttgtggtc
     3361 gcccgcgtag gtgccggtcg cgtcggtgcg cccgtagtcg tcgtcgggcg gcgggtcgag
     3421 cgacggcggg tccatgatcg ccggggtcga gccgatcacg gcgcccaggg gctgccggcg
     3481 gacgtagccg atcaggtctc tcgcctcaac gttcgacgtg ttgagggggg tcgcgttgtt
     3541 ggtcggtccg aggaacggcg cgatcagcca gtggttctcg ggcgtgaacg ccaccatccc
     3601 cgccgtgggg atgtaggtgt agatgttgcg ggagtccggg ttcctcggca ccctcgcgag
     3661 gttcgccagc tcgggccttc catcgaggtc gggccacagg ggcgtgccgt ccttggtgaa
     3721 ggtgtagccg gacggcttgg tgctgtcggg gatcggcttg aacacccgga aggcgcgcag
     3781 gcggccgtag aagcccggcc cgtccgggaa ggcgtgcgcg tcagcgaggc cgagcccgaa
     3841 acccgcggtg agcatgaagt tgttgcgctg ggggatgacg tccccggtgg ccgtgtagac
     3901 ggtgtggttc accagcaagg tcgccgtcga gtcgctggcc tcctcgaggt tcaccgtgcc
     3961 gaccacgggg ctcaccggga agtactcgct ggaccggcgc tcggtctgcg cgttgaaatc
     4021 gggggggcga ctgaagccgg cctggagggc gaggttgacc gcgcgcgcca cgccggggcg
     4081 gttccaggcg cggatgagca cgccgccgct cgagtcggcg atggtctgca gctgcacctg
     4141 gtcggccgcg tagttcggcg actgggggtt cgggcgcacc cagacgacga agatcggcac
     4201 cttgtggccg cctcctgccg cgagcgcctt gaactccgcc gccttggccg aggggtcggc
     4261 atggtggtcg cggcctcccg tcaccagcac cacgaccgtg ttgcggcagt tcgagcaggc
     4321 ggtgtcgttc acgatggcgc tcgacgcggc cgacttgacg tcgccgagca actgcgtcag
     4381 cggccggtcc tcgaagccct gcgtgtcgag cccggccggg atcaacagcg ggttggtgtc
     4441 cgccccgttg gtcgtgtcgg tgccaaggac gttgagaacg ctctgggcgg tccccgcggc
     4501 cggcggcacc agcgtcgaac tggtgctgag gcccgtggag ttgaggccgg ccacgctggt
     4561 ggcgaagatg ccgagcaggc cgccgccgtc gcatgcgccc gtgtcggcga tgccggccag
     4621 cggcgagccg gacggcacga ccaccggcgc gtcgcaggtc gtgcggcggg cgggctgg
//
LOCUS       DAOPQX010000297         4665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20011_length_4665_cov_4.583731, whole genome shotgun sequence.
ACCESSION   DAOPQX010000297 DAOPQX010000000
VERSION     DAOPQX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20011_length_4665_cov_4.583731"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKK95_13670"
     CDS             <1..754
                     /locus_tag="PKK95_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC19316.1"
                     /translation="PVVPGFAHLDVPPDSRILSQFDDGWTNVLFVGRVIPNKRIDQVI
                     RIFAAYQRFFNPRSRLLIVGSHTSFERYLAMLHGLVARLGARGVHFLGHVSNRELTAC
                     YDVADVFLCASEHEGFCVPLVESFYKRVPVLAYAATAVPVTLDGAGVLYSSTEPAHVA
                     GLVDEVASDPALVDRICEAQDAALGRLLAKDFRGTLLGFVEGVAQAPRPERPPVADDF
                     WEQVASTEAYEELQQYRPAAFHALPKEPTRVV"
     gene            778..1839
                     /locus_tag="PKK95_13675"
     CDS             778..1839
                     /locus_tag="PKK95_13675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC19317.1"
                     /translation="MRVNQWVPAAHRGDAIGDSARRVQALLRGMGHESELYALTVDED
                     LKDVVRPFSDPASRAGDATIFHFALPSPMTEAFAALPRGRVLQYHNITPAHFFAPYDP
                     ALFRLASLGRAELAALAGRTDLALGDSEYNRRELEALGFRDTGVMPIAIDTGRITGAP
                     RRPALERILGDGLANFLFVGRVVPNKKIEDHIRLAEHYKRYVSVDYRFVFVGRCDAVP
                     RYDAAVRAMILEYDMPPDRFLFTGPVPDEDLAAYYRTATAYVSLSEHEGFCVPLLEAM
                     AADVPVLAYGAAAVPDTLGGAGLQFYPKDLEHAAELLGLLAEDEALRARVIAGQRERL
                     RDFGDARIERDLRAIVERL"
     gene            1836..3047
                     /locus_tag="PKK95_13680"
     CDS             1836..3047
                     /locus_tag="PKK95_13680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOC19318.1"
                     /translation="MKLAFIVQRYGTEILGGSEYHCRLIAERLAQRHQVEVLTTCARD
                     YITWANEYPEGADRVRGVTVRRFPNARTRDIDSFNRFSDWIFSNPHEVGDEMEWLQQQ
                     GPWCPSLLEYLHARHASYDALIFFTYLYAPTVLGLNVAPGRSILVPTAHDEPAIGLSI
                     YRDVFALPAAIAYNTAAERRFLTTRFAIRAVAEETVGCGVDLPQHRGYPRGPSTSGLS
                     GRGATFRRRHRLYRPFVLYGGRIDRGKGCEELIEYFNTYVSEGGEASLVLMGVKLMPL
                     PEDPAIKFAGLLSEVERAQALEAATVVAVPSPYESLSLLALEAFSVGTPVLANARSEV
                     LVDHCVASNAGLYYADRYEFVEALDLLTRDEPLRAAMGRNGRDYVRQHYRWDVILAKY
                     ERMIGKIRSAG"
     gene            complement(3069..4508)
                     /locus_tag="PKK95_13685"
     CDS             complement(3069..4508)
                     /locus_tag="PKK95_13685"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC19319.1"
                     /translation="MPSRTGTVLVVDDEEIMREILETLLTREGYEVRLASGGAEALEI
                     ARTVPVDAAIVDVMMPGMDGLTTLDEIKKIDEEIGVIMITAFASVETAIAAMKRGAFD
                     YITKPFKHDEVLVVLRNAVERRQLVAENVALRQNLQERYSRFQNIIGRSQRMRQVFDL
                     IIQAAPSRSTILIGGESGTGKELVARAIHSNSPRAARPFVTVNSGSLPPDLLESNLFG
                     HVKGAFTGAVYPKKGLFELADKGTIFFDEIGNVALETQPKLLRVMQEREFMRLGGVET
                     LKVDVRIVAATNVDLRQMVEEQRFREDLYYRLHVITIPLPPLRERKEDIPLLVQHFLN
                     KYGEENQKQGLELVPEALDLLMEYDWPGNVRELENVIERAVVLSTASRIGSDLIPEQV
                     RNRTVFRLPQFSLPSEGIDFNQVTADVQRHLIESALEAAGGVQKRAAELLRLKPTTLN
                     EMIKRYDIRSRRGRQRPENGDEAAAAEVQ"
BASE COUNT          635 a         1675 c         1619 g          736 t
ORIGIN      
        1 cccggtggtg cccggctttg cgcacctcga cgtgccgccc gacagccgca tcctgagcca
       61 gttcgacgac gggtggacga acgtgctgtt cgtgggccgg gtgatcccga acaagcggat
      121 cgaccaggtg atccggatct tcgcggcgta ccagcgcttc ttcaacccgc gctcgcggct
      181 gctgatcgtc ggcagccaca cgagcttcga gcggtacctg gcgatgctcc acgggctggt
      241 ggcccggctc ggcgcccgcg gcgtccactt cctcggccac gtctcgaacc gcgagctcac
      301 cgcgtgctac gacgtggccg acgtcttcct ctgcgcgagc gagcacgagg ggttctgcgt
      361 cccgctcgtg gagtcgttct acaagcgggt gccggtgctg gcgtacgcgg cgacggccgt
      421 gccggtgacg ctcgacgggg ccggcgtgct ctactcctcg accgagcccg cgcacgtggc
      481 ggggctcgtc gacgaggtgg cgtctgaccc ggccctcgtt gaccgtatct gcgaggcgca
      541 ggatgccgcc ctcggccgcc tgctcgccaa ggactttcgc ggcaccctcc tcggcttcgt
      601 ggaaggggtc gcgcaggccc cgcggcccga gcggccgccc gtcgccgacg atttctggga
      661 gcaggtcgcc tccaccgagg cgtacgagga gctgcagcag taccgccccg cggccttcca
      721 cgcgctgccc aaggaaccga ccagggtggt gtaggggaac ggggtggcag ggtctcgatg
      781 agagtgaacc agtgggtccc cgcggcgcac cggggggacg cgatcggcga cagcgcccgg
      841 cgcgtgcagg cgctcctgcg gggcatgggc cacgagtcgg agctgtacgc gctgaccgtg
      901 gacgaggacc tcaaggacgt ggtgcgcccc ttctcggacc cggcgtcgcg cgcgggggac
      961 gcgaccatct tccacttcgc gctcccctcg ccgatgaccg aggcgttcgc cgcgctgcct
     1021 cgggggcgcg tcctccagta ccacaacatc acgccggcac acttcttcgc gccgtacgac
     1081 ccggcgctgt tccgcctcgc gtcgctcggc cgcgccgagc tggccgcgct ggccggccgc
     1141 accgacctcg ccctgggcga ctccgagtac aaccgccgcg agctcgaggc gctgggcttc
     1201 cgcgacaccg gtgtgatgcc gatcgcgatc gacaccgggc ggatcaccgg ggccccgcgc
     1261 cgcccggccc tcgagcggat cctcggcgac gggctggcga acttcctgtt cgtcgggcgc
     1321 gtggtgccca acaagaagat cgaggaccac atccgcctgg ccgagcacta caagcggtac
     1381 gtgagcgtgg actaccggtt cgtgttcgtc ggccggtgcg acgccgtccc gcgctacgac
     1441 gcggcggtgc gggcgatgat cctcgagtac gacatgccgc ccgaccgctt cctcttcacg
     1501 gggccggtcc ctgacgagga cctggcggcg tactaccgga cggccaccgc ctacgtctcg
     1561 ctcagcgagc acgaggggtt ctgcgtcccc ctgctcgagg cgatggccgc cgacgtgccg
     1621 gtgctggcct acggcgcggc cgccgtgccc gacacgctgg gaggggccgg gctgcagttc
     1681 tatccgaagg acctcgagca cgccgccgag ctgctcggcc tgctggccga agacgaggcg
     1741 ctgcgcgccc gcgtcatcgc cggccagcgc gagcgcctgc gcgacttcgg cgacgcgcgc
     1801 atcgagcgcg acctccgcgc catcgtggag cgcctgtgaa gcttgcgttc atcgtccagc
     1861 ggtacggcac ggagatcctc gggggctcgg agtaccactg ccgcctcatc gccgagcgcc
     1921 tggcccagcg ccaccaggtc gaggtgctga ccacctgcgc ccgcgactac atcacctggg
     1981 ccaacgagta cccggaaggc gccgaccgcg tgcggggggt caccgtccgg cgcttcccga
     2041 acgcccgcac ccgcgacatc gactcgttca accggttctc ggattggatc ttctcgaacc
     2101 cgcacgaggt cggcgacgag atggagtggc tgcagcagca gggcccgtgg tgcccgtcgc
     2161 tcctcgagta cctgcacgca cgccacgcct cgtacgacgc cctgatcttc ttcacctacc
     2221 tctacgcgcc gaccgtgctc ggcctcaacg tcgcccccgg gcgcagcatc ctggtcccca
     2281 ccgcgcacga cgagccggcc atcggcctct cgatctaccg cgacgtcttc gcgctgcccg
     2341 cggcgatcgc gtacaacacg gccgccgagc ggcgcttcct gacgacgcgc ttcgcgatcc
     2401 gggcggtcgc cgaggagacg gtcgggtgcg gcgtggacct gccccagcac cgcggctacc
     2461 cccgcggccc ctccacgtcg ggcctgagcg gccgcggcgc gaccttccgg cggcgccacc
     2521 gcctctaccg gccgttcgtc ctctacggcg ggcgaatcga ccgcgggaag gggtgcgagg
     2581 agctgatcga gtacttcaac acctacgtga gcgagggggg cgaggcgtcg ctggtgctga
     2641 tgggcgtcaa gctgatgccc ctgcccgagg atcccgccat caagttcgcc ggcctgctgt
     2701 cggaggtcga gcgggcgcag gcgctcgagg cggccaccgt cgtggccgtg ccgtcgccct
     2761 acgagagcct gtcgctgctc gcgctggagg cgttctcggt cgggacgccg gtgctggcca
     2821 acgcgcggtc ggaagtgctc gtcgatcact gcgtggccag caacgccggc ctctactacg
     2881 ccgatcgcta cgagttcgtc gaggcgctcg acctgttgac ccgcgacgag ccgctgcgcg
     2941 ccgcgatggg gcggaacggc cgcgactacg tccggcagca ctaccggtgg gacgtcatcc
     3001 tggccaagta cgaaaggatg atcgggaaga tccggtcggc cggctagtcc cgccggggcc
     3061 gcggctggct actgcacctc ggcggccgcc gcctcgtcgc cgttctccgg gcgctggcgg
     3121 ccgcgccgcg accggatgtc gtagcgcttg atcatctcgt tgagcgtggt gggcttgagg
     3181 cgcagcagtt cggcggcgcg tttctggacg ccgccggccg cctcgagcgc cgactcgatc
     3241 aggtggcgct ggacgtcggc ggtgacctgg ttgaagtcga tcccctccga gggcagcgag
     3301 aactgcggca ggcggaacac cgtccggttg cggacctgct cggggatcag gtccgacccg
     3361 atccgcgagg cggtgctcag caccaccgcc cgctcgatga cgttctccag ctcccgcacg
     3421 ttgcccggcc agtcgtactc catcagcagg tcgagcgcct cgggcaccag ctcgagtccc
     3481 tgcttctggt tctcctcccc gtacttgttg aggaagtgct ggacgagcag cgggatgtcc
     3541 tccttgcgct cgcgaagcgg cggcagcggg atcgtgatga cgtgcaggcg gtagtagagg
     3601 tcctcgcgga agcgctgttc ctcgaccatc tgccgcaagt ccacgttggt cgccgcgacg
     3661 atgcggacgt cgaccttcag ggtctcgacg ccgccgaggc gcatgaactc gcgctcctgc
     3721 atcacccgca gcagcttggg ctgggtctcg agggcgacgt tgccgatctc gtcgaagaag
     3781 atcgtgccct tgtcggccag ctcgaacagc cccttcttcg ggtagaccgc cccggtgaag
     3841 gctcccttca cgtggccgaa caggttcgac tcgagcaggt cgggggggag gctgcccgag
     3901 ttgacggtca cgaagggccg cgcggcgcgc ggcgagttcg agtggatcgc ccgcgccacc
     3961 agctccttgc cggtgccgct ctcgccgccg atcaggatgg tcgagcggct gggcgcggcc
     4021 tggatgatca ggtcgaacac ctgccgcatc cgctggctgc ggccgatgat gttctggaac
     4081 cggctgtagc gctcctgcag gttctggcgc agcgccacgt tctcggcgac cagctggcgg
     4141 cgctcgacgg cgttccggag cacgacgagc acctcgtcgt gcttgaacgg cttggtgatg
     4201 tagtcgaagg cgccgcgctt catcgcggcg atggcggtct cgaccgaggc gaacgcggtg
     4261 atcatgatca ccccgatctc ctcgtcgatc ttcttgatct cgtcgagcgt ggtgaggccg
     4321 tccatccccg gcatcatcac gtcgacgatc gcggcgtcca ccggcaccgt gcgcgcgatc
     4381 tcgagcgcct cggcgccccc ggaggccagg cgcacctcgt agccctcccg cgtcaggagg
     4441 gtctcgagga tctcgcgcat gatctcctcg tcgtcgacta cgagaaccgt accggtgcgg
     4501 gaaggcatgg tcgcgggttc cagccgggac tacccggccc ggtgcgggcg cgacgccgcc
     4561 ggggccgcgg cgggaagcgt cagggtgaac cgggtgccca ccccgacctg gctctcgcaa
     4621 ttgatggccc cgtcgtgctc gcgcacgatt ccataggtca ccgag
//
LOCUS       DAOPQX010000298         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20069_length_4653_cov_5.597869, whole genome shotgun sequence.
ACCESSION   DAOPQX010000298 DAOPQX010000000
VERSION     DAOPQX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20069_length_4653_cov_5.597869"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="PKK95_13690"
     CDS             complement(<1..1725)
                     /locus_tag="PKK95_13690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC19320.1"
                     /translation="MTPAPVAVVITCYNLGRTLREAAASVLWQTRPPAEWLIVDDGSD
                     DLYTLQEEVRLQDEGWRILRTPNRGVSAARNAGVRATSSPWLVLLDADDVLEPSYLER
                     MLARAEEDPALDFVSCSMQAFGASAYVWRPPDPGIPDSCIWGVVHNSSLFARRIWETV
                     GGFDEAPGPERESDFWMSALEHGFRGAVVDEPLLRYRVREASLYHQAIDPAAHERLME
                     AFYRNHWHAVSRHAEEMLVGKEAFLLSLSEHLAHVSGEVGRLRGEVDRLRSRIADSVG
                     ELQQLGEPPFDMGEARRTTPVSARWGVERGTPIDRVYIEQFLERHKGNIRGRVLEVKD
                     PGYTLRFGGEAVTACDVIDVDPLNARASIHADLSRAGAIPAEAYDAFVLTQTLGLVYD
                     VPAALRHAFRILKPGGVLLCTVPAAGRLSHEDRGLDGDYWRFTEASLRRLFAEVFPLE
                     AIEITGFGNVLADAAFLYGLAAHELSPSELAATDPCLPLVYGVRAVKPRAAGGANPGA
                     APLPGAEASEPAGPHARTRSRRGVILAYHRVSSAGGAAPRAVSTPAFRAQMTFLREHC
                     RPLPLARLA"
     gene            complement(1722..2732)
                     /locus_tag="PKK95_13695"
     CDS             complement(1722..2732)
                     /locus_tag="PKK95_13695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOC19321.1"
                     /translation="MNPPSISVIIPCFNLGQYLDEAVESVLAQTRQDFEILIVDDGST
                     DPGTERLLAGYARPKTRVWRTTNRGLAAARNFLIERATAKYLCALDADDRLHPQYFER
                     ALDAFASDPGLAFVSSWVQMFGEEDTVWRRDRCDLPALLAEDTVMTAALVRRDAVVAL
                     GGYDERMPHQGDEDWDLWIRLVRAGWRGTILPETLFFYRRRPGSMSTCCTRGQVHLDL
                     VRYLARKHADAYREHLREVLLSQDRRIAGLLRATDALERERDGQLRTTRAALEAELDR
                     LSARLASVSERRQGLWDLQEECERLRAEVAALRGSLTWRLTAPARAAYDLIAAPFRRS
                     RP"
     gene            complement(2729..>4653)
                     /locus_tag="PKK95_13700"
     CDS             complement(2729..>4653)
                     /locus_tag="PKK95_13700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC19322.1"
                     /translation="PSGPPLVSVVVVLLNRAPLTFGCLTALLAASPPWVELVMVDNGS
                     TDSTSRLLQRVRGATIVRNERNLGFAAAVNQGARAGSGQYLLLLNNDAQLLPGSLEAA
                     LRRLETRSDAGAVGGRLVWPDGRLQEAGSVVFDDGTCRGYGRGARALDGEYMFARDVD
                     FCSGAFLLTARQTFMESGGFDEVFSPAYYEDVDYCVRLRGAGRLILYEPRAAAIHFEF
                     GSATSVPEALEAQATGRKRFVEKHRDWLRRQMAPGSILQARSRRGEGLRVVVFDDRVP
                     HPELGSGLGRSAALLRALVELGHFVTFYPLGVTEEEWGRVYHVLPDTVEVMLGRGAGG
                     VGDFVRERRGYYDCTIVSRAPNMRLLQAALRAQGAWPPSKALIYDAEAVAACRELARR
                     RVAGEPVQATEAARLLSEELQLGASAGLVFSVSARERERFVEAGIEDVRVLSYPIEPT
                     PTRAPFDERLGLLFAGGFSAPSPSADSVTWFAAQVLPRLRAAAGADLRFCVAGATPPP
                     EVARLTGQGVDILGCVEDLTALYDRSRVFVAPTRFAAGIPLKVLDAAAHGLPVVCTSL
                     LADQLGWRDGEQLLVADDPATFADCCLALHHDAALWHRLRRAALDRIALDCSPEAFRD
                     TLRQALTSLITSRAPR"
BASE COUNT          727 a         1646 c         1660 g          620 t
ORIGIN      
        1 ggccagccgc gcgagcggca gcggccggca gtgctcgcgg aggaaggtca tctgcgcgcg
       61 gaaggccggc gtcgaaaccg cgcgcggcgc ggcgcccccc gccgacgaga cgcggtggta
      121 ggcgaggatc acgccgcgcc ggctccgggt ccgggcgtgc gggccggccg gctcgctcgc
      181 ttcggcgccg ggcaagggag cagctcccgg gttggccccg ccggccgcgc gcggcttcac
      241 cgcgcggacg ccgtagacga gcggaagaca cgggtcggtt gcggccagct cggagggaga
      301 cagctcgtgg gcggcgaggc catacaggaa cgcggcgtcg gcgagcacgt tgccgaaccc
      361 ggtgatctcg atcgcctcca gagggaagac ctccgcgaag agtcggcgca gcgaggcctc
      421 ggtgaaccgc cagtaatcgc cgtccagtcc ccggtcctcg tgcgagaggc ggccggccgc
      481 cgggaccgtg cacaagagca cgccgccggg cttcaggatc cggaatgcgt ggcggagcgc
      541 ggcggggacg tcgtagacga ggcccagtgt ctgcgtgagg acgaacgcgt cgtaggcctc
      601 ggccgggatg gcgccggcgc gggacaggtc ggcgtggatg gaggcgcgcg cgttgagcgg
      661 atcgacgtcg atcacatcgc aggcggtgac cgcctcgccg ccgaaccgga gggtgtagcc
      721 ggggtccttg acctcgagga ccctcccgcg gatgttgccc ttgtgccgct cgaggaactg
      781 ctcgatgtac acgcggtcga tgggagtccc ccgctcgacg ccccacctcg cgctgacggg
      841 cgtcgtgcga cgcgcttcgc ccatgtcgaa gggcggctcg ccgagctgct gcagttcccc
      901 cacggagtcg gcgatcctcg accgcagccg atccacctca cctcgcagcc ggccgacctc
      961 ccccgacacg tgagccagat gttcgctcag cgagagcagg aaggcctcct tgccgaccag
     1021 catctcctcg gcgtgacggc tcaccgcgtg ccagtggttg cggtagaacg cctccatcag
     1081 ccgctcgtgc gcggccgggt cgattgcctg gtgatacagg gaggcctcac gaacgcggta
     1141 ccggagcagg ggctcgtcga cgaccgctcc ccggaagccg tgctcgagcg cgctcatcca
     1201 gaagtccgac tcgcgctccg gcccgggcgc ctcgtcgaaa ccgcccacgg tctcccagat
     1261 cctccgcgcg aagagagagg agttgtggac gacaccccag atgcaggagt cggggatgcc
     1321 gggatccggc gggcgccaca cgtaggccga cgcaccgaac gcctgcatcg agcacgagac
     1381 gaagtccagc gccgggtcct cttcggcccg agccagcatc cgttccaggt acgagggctc
     1441 caacacgtcg tccgcgtcca gcagcaccag ccagggagaa gaggtagccc gcacgcccgc
     1501 gtttcgcgcg gcggagacgc cgcgattcgg agtgcggagt atccgccagc cctcgtcctg
     1561 gagcctcacc tcctcctgga gcgtgtagag gtcgtccgag ccgtcgtcca cgatcagcca
     1621 ctccgccggc gggcgggtct gccacaggac gctggcggcg gcctcccgga gcgtgcggcc
     1681 caggttgtag caggtgatga cgacggcaac cggtgcgggc gtcacgggcg actccgccgg
     1741 aacggggccg caatcaggtc gtaggcggcg cgtgccgggg ccgtgagccg ccaggtgagc
     1801 gagccgcgca gggccgccac ctcggcgcga agccgctcgc actcctcctg caggtcccat
     1861 agcccctggc gtcgctccga cacgctggcc agcctggcgc tcaagcggtc cagctctgcc
     1921 tcgagggccg cccgcgtcgt cctcagctgg ccgtcccgct cgcgctcgag ggcatcggtc
     1981 gcccgaagca ggccggcgat gcggcgatcc tgcgagagga gcacctcgcg caggtgctcg
     2041 cggtaggcgt cggcgtgctt gcgcgccagg tagcgcacga gatcgaggtg gacctggccg
     2101 cgcgtgcaac acgtgctcat cgaccctggg cgcctgcggt agaagaaaag ggtctcgggc
     2161 aggatcgtcc cgcgccagcc ggcccgcacc aggcggatcc agaggtccca gtcctcgtcg
     2221 ccctggtggg gcatccgttc gtcgtacccg ccgagcgcga cgacggcgtc ccttcggacg
     2281 agcgccgccg tcatcaccgt gtcctccgcg agcagcgccg ggaggtcgca gcggtcccgg
     2341 cgccacacgg tgtcctcttc gccgaacatc tggacccagc tcgagacgaa ggcgagcccc
     2401 gggtcggacg cgaaggcgtc cagcgccctc tcgaagtact gcgggtggag cctgtcatcg
     2461 gcatcgagcg cgcacaagta cttggcggtc gctctctcga tgaggaagtt ccgggcggca
     2521 gcgaggcctc ggttcgtggt tcgccagacc ctcgtcttcg gccgcgcgta gccggccagc
     2581 aggcgctcgg ttccgggatc ggtggagccg tcgtccacga tcaggatctc gaagtcctgg
     2641 cgcgtctgtg cgaggacgct ctcgacggcc tcgtcgaggt actggccgag gttgaagcac
     2701 gggatgatga ccgagatgct gggcgggttc accgcggtgc cctcgaggtg atgagggagg
     2761 tgagggcttg acgaagggtg tcgcggaaag cctcgggcga acagtcgaga gcgatgcggt
     2821 cgagggccgc acggcgcagc cggtgccaga gcgccgcatc gtggtggagc gcgaggcagc
     2881 agtccgcgaa cgtggcggga tcgtcggcca ccagcagctg ctccccatcg cgccagccga
     2941 gctggtctgc gagcagcgac gtgcagacga ccggcaaccc gtgcgccgcc gcgtcgagta
     3001 ccttgagggg gatgccggcc gcgaacctgg tcggcgcgac gaagacgcgc gagcggtcgt
     3061 agagggcggt cagatcctcg acgcacccca ggatatcgac gccctgcccg gtcaaccgtg
     3121 ccacctcggg cggaggagtc gcgccggcga cgcagaaccg cagatcggcg cccgctgccg
     3181 ctctgagcct cgggagcacc tgcgcggcga accaggtgac ggaatcggcg ctcggggagg
     3241 gcgcgctgaa ccccccggcg aagagcagtc cgagccgctc gtcgaaaggc gcccgcgtcg
     3301 gcgtcggctc gattgggtag gaaagtaccc ggacatcctc gatcccggcc tccacgaacc
     3361 gctcccgctc gcgtgcggac acggagaata cgagtccagc gctcgcgccg agctgcagtt
     3421 cctcggacag gagtcgtgcg gcctcggtgg cctgcaccgg ctctccagcc acgcggcggc
     3481 gcgccaactc cctgcaggcc gccaccgcct ccgcatcata gatcagggcc ttggaaggag
     3541 gccacgcgcc ctgcgcgcgc aacgcggcct ggagcagccg catgttgggg gccctgctga
     3601 cgatcgtgca gtcgtagtaa ccgcggcgct cccggacgaa gtcaccgact ccgccggccc
     3661 cgcggcccag catcacctcg accgtgtcgg gcagaacgtg gtagacacgt ccccattcct
     3721 cttccgtcac gccgagcggg tagaacgtca cgaaatggcc gagctcgacg agcgcccgca
     3781 gcagcgcggc cgagcggccg agacccgagc cgagctctgg atgcggaacg cgatcgtcga
     3841 agacgacgac tcgcagccct tctccgcgac ggctgcgagc ctgcaggatg ctgccgggcg
     3901 ccatctgccg gcgtagccaa tcgcggtgct tctcgacgaa tcgcttccgc ccagtggcct
     3961 gcgcttcgag ggcctcgggc accgacgtgg cgctcccgaa ctcgaagtgg atggcggcgg
     4021 cacgcggttc gtacaggatc agccgcccag caccccgaag gcggacgcag tagtccacgt
     4081 cttcgtagta cgccgggctg aacacctcgt cgaatccgcc ggactccatg aacgtctgcc
     4141 gggccgtcag caggaaggcg cccgagcaga agtccacgtc cctggcgaac atgtactccc
     4201 cgtcgagcgc tcgcgcgccc cggccgtatc cgcggcaggt tccgtcgtcg aagaccacgc
     4261 tgccggcctc ctgcagccgg ccgtcgggcc agacgagccg cccgccgacg gcccccgcat
     4321 ccgagcgcgt ctcgaggcgc cggagcgccg cttccagact gcctggcagc agttgcgcat
     4381 cgttgttcag caagagcagg tactgcccgc tcccggcacg ggccccctgg ttcaccgcgg
     4441 cggcgaatcc gaggttccgc tcgttgcgca cgatcgtcgc accgcgtacc ctctgcagca
     4501 accgagaggt cgagtcggtg gacccgttgt ccaccatgac gagctccacc cagggaggcg
     4561 atgcggcgag gagcgctgtg aggcacccga aggtgagcgg cgcccgattg agcaggacca
     4621 ccaccacgct cacgagaggg ggcccgctcg gag
//
LOCUS       DAOPQX010000299         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20185_length_4632_cov_3.686476, whole genome shotgun sequence.
ACCESSION   DAOPQX010000299 DAOPQX010000000
VERSION     DAOPQX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20185_length_4632_cov_3.686476"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PKK95_13705"
     CDS             <1..713
                     /locus_tag="PKK95_13705"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOC19323.1"
                     /translation="LAGPVPFRIGIVGGMGPMAGVYLQRLIVEATPAGRDQDHLEVVC
                     FTNPHIPERMRSLAEDGGRRYAEAVRAAAALVARAGATCIAIPCNTAHARLKEIQEGI
                     EVPILDMVALGLDALVAAHGPGRRVGLLATIGTLAEQVYEGRASGRVAEWVRPAPADQ
                     QLVADAILEVKLGRPAEAAPILLPVVGRLRDRGAEVVMVACTELSLCHEALEGAGVPL
                     LDPLRVLARHLVELGARG"
     gene            780..1667
                     /locus_tag="PKK95_13710"
     CDS             780..1667
                     /locus_tag="PKK95_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOC19324.1"
                     /translation="MGATIYVNGRITGESEAVIPVLDHGFLYGEGVYEVMRTYGRAPF
                     LYGRHMARMRRSAAMIDLEVPFTDEYLLGEIRKTADRFYQGQGPEGDGLEMYIRVLLT
                     RGAGAITYDVRSCPAPTVVIIVKPQVDPPPEAYDQGVKVILVPVVRNHPGSVNPLIKS
                     NNLLNNALAWQQATRQGGFEAIMRNYRGEVSECSQSNLFVVKGGEAVTPPLEAGLLAG
                     ITREFVFECGESAGIPVRETTLHDDDLFGADELFLTSTTREIVPIVQVDDRKVGTGRP
                     GPATRKLLAEFRRRIPEGL"
     gene            complement(1719..3185)
                     /gene="gcvPB"
                     /locus_tag="PKK95_13715"
     CDS             complement(1719..3185)
                     /gene="gcvPB"
                     /locus_tag="PKK95_13715"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOC19325.1"
                     /translation="MTTPDRRDELVFNLTSPGRRGYSLPARDVPEAPLEGALPEGLAR
                     EAVEGFPELTEVGLVRHFTRLSTWNYGVDTGMYPLGSCTMKYNPKLNEAAARMPGFAA
                     LHPLTPDEASQGALGVMYALAQMLAEITGLPAVTLQPAAGAHGELTGMMMIRKHLITR
                     DGSPRKLVLIPDSAHGTNPASAHFCGYQVREIKSSPRGTLSVSALAEAMTDEVAALML
                     TNPNTLGIFEEEICEVARIVHAKGGLVYCDGANMNALVGRARPGDFGMDIMHLNLHKT
                     FSTPHGGGGPGSGPVVAIKALEPYLPTPVIVATPDGLRRDYDRPLSVGRVKAFQGNFG
                     MVLRAYAYIRTLGPDGIRATADNAVLNANYLRVKLRDDYDVPYDRLNMHEVIFSDHHQ
                     NAHGVTTLDIAKRLIDYGFHPPTVYFPLIVHGALMVEPTESEGLEELDRFVEVMKRIA
                     EEARTEPGLLKKAPVTTRRSRFDEAAAARKPVLRWKKE"
     gene            complement(3201..4541)
                     /gene="gcvPA"
                     /locus_tag="PKK95_13720"
     CDS             complement(3201..4541)
                     /gene="gcvPA"
                     /locus_tag="PKK95_13720"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOC19326.1"
                     /translation="MTHRYIPNTEEDARRMLEAIGVSSIDALFEPVPERVRLQRALDL
                     PPAASEPDLMAHLAALAAQNADATRYAVFLGAGAYRHVAPAFVDQLLLRSEFYTAYTP
                     YQPEISQGTLQAIFEFQTLVSLLFGTDLANASMYDGSTAAAEGATMACRMTRRDRIVV
                     AKTVHPHYREVLVTYAANLGLEIVEVGYREDGTLDTQALEEASRGAAVVLFQYPNFFG
                     IVEDPRPIVELARRAGAMTVAAVTEPVAMGLLAPPGALGIDIVAAELQAFGVPVGFGG
                     PHVGVLAASEKCMRQMPGRFVGQARDTEGRIGYVLTLATREQHIRREKATSNICTNSG
                     LMALAATICMAAYGKEGLSELARLNFDKAHYAHAALAAAGLRPRFAAPFFNEFVVGGL
                     GDAAAVQHRLLDRQIVAGLPLGPYYPELADSLLVAVTEANPVQEIDALARALKG"
BASE COUNT          668 a         1617 c         1650 g          697 t
ORIGIN      
        1 ccctcgccgg gcccgtcccc ttccgcatcg ggatcgtcgg cggcatgggg ccgatggccg
       61 gggtctacct gcagcggctg atcgtggagg cgacgccggc cgggcgcgac caggatcatc
      121 tcgaggtggt gtgcttcacc aacccgcaca tccccgagcg catgaggtcg ctcgccgagg
      181 acggcggccg gcggtacgcg gaggccgtgc gagccgccgc agcgctggtg gcacgcgccg
      241 gagcaacctg catcgcgatt ccctgcaata ccgcccacgc caggctgaaa gaaatccagg
      301 aggggatcga ggtcccgatc ctcgacatgg tggccctcgg actcgacgcg ctggtcgcgg
      361 cgcacgggcc gggccgccgg gtcggcctgc tggccacgat cggcacgctg gccgagcagg
      421 tgtacgaagg gcgggcgagc ggccgcgtgg ccgagtgggt gcggccggcg ccggccgatc
      481 agcagctcgt cgccgacgca atcctcgagg tgaagctcgg ccggcccgcg gaggccgcgc
      541 cgatcctgct gccggtcgtc ggccggctcc gcgatcgtgg ggccgaggtc gtgatggtcg
      601 cctgcacgga gctgtcgctg tgccacgagg cgctcgaagg ggccggtgtc ccgcttctgg
      661 accccctgcg cgtcctcgcg cggcacctgg tggagctagg ggctaggggc taggggctag
      721 gggctagggc tcggggctag gcgccgaggt cggcggggcc gcgtattctg gagggctcga
      781 tgggagcgac gatttacgtg aacggccgca tcaccggaga gagcgaggct gtcatcccgg
      841 tgctcgacca tggattcctg tacggcgaag gcgtgtacga ggtgatgcgc acctacggcc
      901 gcgccccgtt cctctacggc cggcacatgg cgcggatgcg ccgctccgcc gcgatgatcg
      961 acctcgaggt ccccttcacc gacgagtacc tcctcggcga gatccggaag actgccgatc
     1021 gcttctacca gggccagggg ccggagggcg acggcctgga gatgtacatc cgcgtcctcc
     1081 tcacgcgcgg cgccggcgcc atcacctacg acgtccgctc ctgccccgcc ccgaccgtcg
     1141 tgatcatcgt gaagccgcag gtggatcccc cgcccgaggc ctacgaccag ggagtgaagg
     1201 tcatcctggt cccggtcgtc cgaaaccacc ccggctcggt caacccgctg atcaagtcga
     1261 acaacctgct gaacaacgcg ctcgcgtggc agcaggccac gcgccagggc ggcttcgagg
     1321 cgatcatgcg caactaccgg ggcgaggtct ccgagtgctc gcagtcgaac ctgttcgtgg
     1381 tcaagggcgg cgaggcggtg acgccacccc tcgaggccgg gctgctggcc gggatcacgc
     1441 gtgaattcgt gttcgagtgc ggagagtcgg caggcatccc ggtccgcgag acgacgctcc
     1501 acgacgacga cctgttcggc gccgacgagc tgttcctgac cagcacgacg cgagagatcg
     1561 tgccgatcgt gcaggttgac gatcggaagg tgggcaccgg acgtcccggc ccggccacca
     1621 ggaagctgct ggcggagttc cgccggagga ttccggaagg actgtgaacg gcggcgaggg
     1681 gcggcgggca ggcggtctct cccgcctgcc cgccccggct attccttctt ccaacgcagc
     1741 acgggcttcc gggcggcggc ggcctcgtcg aagcggctgc ggcgggtggt gaccggcgcc
     1801 ttcttgagca ggccgggctc ggtgcgggcc tcttccgcga tccgcttcat cacctccacg
     1861 aaccgatcca gctcctccag cccctcgctc tcggtcggct cgaccatcag cgcgccgtgc
     1921 acgatgagcg ggaagtacac cgtcggcggg tggaagccgt agtcgatcag gcgcttggcg
     1981 atgtcgagcg tcgtcacgcc gtgggcgttc tggtggtggt cggagaagat cacctcgtgc
     2041 atgttgaggc ggtcgtacgg cacgtcgtag tcgtcgcgaa gcttcacccg caggtagttc
     2101 gcgttcagca ccgcgttgtc ggccgtcgcc cggatgccgt cgggcccgag cgtccggatg
     2161 tacgcgtagg cgcgcagcac catcccgaaa ttaccctgga acgccttcac gcggcccacc
     2221 gacagcgggc gatcgtagtc ccggcgcagc ccgtcggggg tggcgacgat cacgggtgtg
     2281 ggcaggtacg gctccagcgc cttgatcgcg accaccgggc ccgaacccgg ccctccgccg
     2341 ccgtgcggcg tcgagaaggt cttgtgcagg ttcaggtgca tgatgtccat gccgaagtcg
     2401 cccggcctcg cccgcccgac gagagcgttc atgttcgcgc cgtcgcagta gaccaggccc
     2461 cccttggcgt ggacgatccg cgccacctcg cagatctcct cctcgaagat cccgagcgtg
     2521 ttcgggttgg tgagcatcag cgcggcgacc tcgtcggtca tcgcctcggc aagggccgac
     2581 accgagaggg tgccgcgggg agacgacttg atctcccgca cctggtaccc gcagaagtgg
     2641 gcgctcgccg ggttggtgcc gtgcgccgag tcgggaatca agacgagctt gcgcgggctg
     2701 ccgtcgcggg tgatgagatg cttgcggatc atcatcatcc cggtcagctc gccgtgggcg
     2761 ccggccgccg gctgcaacgt caccgcaggc aggccggtga tctcggccag catctgcgcg
     2821 agcgcgtaca tgacgcccag ggcgccctgc gacgcctcgt ccggcgtcag cgggtgaagc
     2881 gcggcgaagc cgggcatcct tgccgcggcc tcgttcagct tcgggttgta cttcatcgtg
     2941 cacgacccga gcgggtacat gccggtgtca accccgtagt tccaggtgga gaggcgcgtg
     3001 aagtggcgga cgaggccgac ctcggtcagc tcgggaaagc cctccacggc ctcgcgcgcc
     3061 aacccctcgg ggagcgcccc ctcgagtggc gcctccggca cgtcgcgcgc cggcagcgag
     3121 tagccccgcc ggcccggcga ggtcaggttg aagacgagtt catcgcgtcg atccggtgtc
     3181 gtcatggtcc cttcgccgcc tcagcccttc agcgcccgcg ccagcgcgtc gatctcctgc
     3241 accgggttcg cctcggtcac cgcgaccagc agcgagtcgg ccagctcggg gtagtacggc
     3301 cccagcggca gccctgccac gatctgacga tcgagcagcc ggtgctggac cgcggccgcg
     3361 tcgcccaggc cgcccaccac gaactcgttg aagaacggcg ccgcgaagcg ggggcgcagc
     3421 cccgcggcgg cgagcgccgc gtgcgcgtag tgcgccttgt cgaagttgag gcgtgccagc
     3481 tcggacagcc cctccttccc ataggcggcc atgcagatgg tcgccgccaa agccatcagc
     3541 cccgagttgg tgcagatgtt cgaggtcgcc ttctcgcgcc ggatgtgctg ctcgcgggtc
     3601 gccagcgtca gcacgtagcc aatccgtccc tcggtgtcgc gcgcctggcc gacgaaccgg
     3661 ccgggcatct gccgcatgca cttctcgctc gccgccagca cccccacgtg cggtccgccg
     3721 aacccgaccg gcacgccgaa ggcctgcagc tcggccgcga cgatgtcgat cccgagcgca
     3781 ccgggaggtg cgagcagccc catcgccacc ggctcggtga ccgccgccac cgtcatcgcg
     3841 ccggcgcgtc tggccagctc cacgatcggg cgggggtctt cgacgatgcc gaagaagttg
     3901 gggtactgga acagcaccac cgcagccccg cgggaagcct cctcgagcgc ctgcgtgtcg
     3961 agcgtgccgt cctcgcggta gcccacctcc acgatctcga gccccaggtt cgcggcgtag
     4021 gtgaccagca cctcgcggta gtgcgggtgc acggtcttcg ccaccacgat gcggtcgcgc
     4081 ctggtcatcc ggcacgccat cgtcgccccc tccgcggcgg cggtgctgcc gtcgtacatg
     4141 gaggcgttgg cgaggtcggt gccgaacagc agggagacga gcgtctggaa ctcgaagatc
     4201 gcctggaggg tgccctgcga gatctccggc tggtagggcg tgtaggcggt gtagaactcc
     4261 gagcgcagca gcagctggtc gacgaaggcg ggggcgacgt gccggtaggc gcccgcgccc
     4321 aggaagaccg cgtaccgggt cgcgtcggcg ttctgcgcgg cgagcgccgc gaggtgcgcc
     4381 atcaagtcgg gctccgaggc ggcgggcggc aggtcgagcg cgcgctggag ccgcacccgc
     4441 tcgggaaccg gctcgaacag cgcgtcgatg ctcgaaaccc cgatggcctc gagcatccgc
     4501 cgcgcgtcct cttccgtgtt gggaatgtag cggtgggtca cttgaccagc tctgcgtact
     4561 gcgcggcgtc catcagctgg gcggcatcgc ccggcgagtc gggctcgatc gccaccatcc
     4621 agctgccgtg cg
//
LOCUS       DAOPQX010000300         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20199_length_4630_cov_3.832787, whole genome shotgun sequence.
ACCESSION   DAOPQX010000300 DAOPQX010000000
VERSION     DAOPQX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20199_length_4630_cov_3.832787"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PKK95_13725"
     CDS             <1..226
                     /locus_tag="PKK95_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19327.1"
                     /translation="RTSGEWWRAPCGTEDGGLPRRSPERGGHPGRVEGQRREGAFDRD
                     EWDVALADGALYRVFQDRATERWFVDGIID"
     gene            304..3621
                     /locus_tag="PKK95_13730"
     CDS             304..3621
                     /locus_tag="PKK95_13730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HOC19328.1"
                     /translation="MYIELHTSSAFSFLDGASLPEALVDRAAELGYPALALIDRDGVY
                     GIPRFHKAATAAGIRPIVGAELTIDGWSLPLLVSSAEGYRNLCGLVTRMKLRAPKGEG
                     ALALDELEGRTAGLVALAGREALRGARHGVGGLLDRLIGLFGRDNVYIELQRHLYRDQ
                     EADRQALRDLASAFHVPLVATNGVRFAAPGDRPLYDVLTCIRHKTTLEAAGRRLTANA
                     ERYLKPPAEMAALFADEPEAVANTEALADRLRYTMADLGYRFPEYPVPPGETAASFLR
                     KMTEVGARERYRPYHDRARAQVARELNLIEKLGLAGYFLIVWDIVNFCRQQGILVQGR
                     GSAANSAVCYSLGITAVDPVGMDLLFERFLSEERGEWPDIDLDLPSGDRRERVIQHVY
                     EKYGEHGAAMTANVITYRGRSAAREVGKVVGLDAATIDRLSKVLHRFEYHDDGETIAK
                     HLGEIGVDPTDHRMRVFAGLWQRIQDLPRHLGQHSGGMVICQGRLDAVVPLENASMPG
                     RVVVQWDKDDCADMGIVKIDLLGLGMMAVLQDSLAAINARRPSAAQPLGLWNLPPDDP
                     EVYRMLRAADTIGVFQVESRAQMATLPRLKPERFYDLVVEVAIIRPGPIVGNMVHPYL
                     NRRAGRETVTYPHPSLEPILARTLGVPLFQEQLLRIAMVAAGFSGGQAEELRRAFGFK
                     RSERRMKQIEVQLREGMARKGITGQAAEDIVKAIASFALYGFPESHSASFALLVYASA
                     YLKAHYPAAFYAALLNNWPMGFYHPATLVKDAQRRGVRFAPVDVQRSHWPCTIEEDGA
                     IRLGFCYVKGLRRQVGEAIEAADSRQQTADSLSRGPRKPERCPKCGCDDESMLEVSRQ
                     GGRLACFCNNCAHDWKVVPPRFRSVDELIARTGINREELSTLAEAGALASLGFTRREA
                     LWQVERAIRPAGELFQDSGFGIRDSGFGIQGSGIRDQRLGIGEQEEDSSPLPEMTPVE
                     RLVADYEGTGLTIGPHPMAMRREEMEMRGALRAIDLAKQRHGRRVRVAGAVITRQRPG
                     TAKGFVFLTLEDETGIANIIVRPDLFARSRLTVVGEPFLLVEGILQNQEGVVSVRAER
                     LDGMGGAKVDVEARDFY"
     gene            complement(3633..>4630)
                     /locus_tag="PKK95_13735"
     CDS             complement(3633..>4630)
                     /locus_tag="PKK95_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOC19329.1"
                     /translation="SWADGSRRWDGLLPPETRPRIIDPPGGRIWTANNQVVYGASPEV
                     YSAAAVDASARARQIRDALLALSKATERDLLHVQMDERPLFLERWRTLLLETLSRPEA
                     RGSAAREEYRRLVASTWTGRASDSVAYTLVQCWRRVLAEQVFGVLTAPARSVMPSFDW
                     VRPADPALPLPPFPTRIEGPLWRLVSERPRHLLSARFESWDEQLLAAVDGAIGELTSD
                     GAKLDELRWGTIDSRIRHPLSAGLGVLARWFDMEEMPLRGGADMPLQRVDGVMPSERL
                     VVSPGHEQDGIFHMPGGQSGHPLSPHYRDGHAAWVNGDPTPLLPGKTAHTLTLTP"
BASE COUNT          690 a         1599 c         1668 g          673 t
ORIGIN      
        1 gcgcacgtcg ggcgagtggt ggcgtgcccc gtgcggaacg gaagacggcg gcttgccgcg
       61 ccgtagcccc gagcgaggcg ggcatccagg ccgggtcgaa gggcaaagac gtgaaggcgc
      121 attcgaccgt gacgagtggg acgtcgcgct cgccgacggc gccctctacc gcgtcttcca
      181 ggaccgcgcg accgagcggt ggttcgtgga cgggattatc gattagcgga gggcgggtgc
      241 gtcggcgaac gccacccgcc gtccgctttt gccgtgcgct gtctgctgtc cgctgtccgc
      301 tgtatgtaca tcgagcttca cacttcctcg gccttcagct tcctcgacgg cgcctcgctg
      361 cccgaggcgc tggtcgaccg tgccgcggag cttggctatc ccgccctcgc cttgatcgat
      421 cgcgacggcg tctacggcat cccccgcttt cacaaggccg ccaccgccgc cggcatcagg
      481 ccgatcgtgg gggcggaact gacgatcgac ggctggtctc tccccctcct cgtctcttcg
      541 gccgaggggt accggaacct ctgcggcctc gtcacccgca tgaagctgcg ggcgccgaag
      601 ggcgagggag cgctcgcgct cgacgagctg gaaggccgaa ctgccggcct ggtggcgctg
      661 gccggccgcg aggcgctccg gggcgcgcgc cacggcgtcg gcggcctgct cgaccggctg
      721 atcggtctct tcggccgcga caacgtctac atcgaactgc agcgccacct ctaccgcgac
      781 caggaggccg accgccaggc gctgcgcgat ctcgcctccg ccttccacgt gccgctcgtc
      841 gccaccaacg gtgtccgctt cgccgccccc ggcgatcgcc cgctctacga cgtgctcacc
      901 tgcatccgcc acaagacgac gctcgaggcc gccggccggc gcctgacggc caacgccgag
      961 cgttacctga aaccgcccgc cgagatggcg gcgctcttcg ccgacgagcc cgaggccgtc
     1021 gcgaacaccg aggcgctcgc cgatcgcctc cgctacacga tggccgatct cggctaccgc
     1081 ttcccggaat atcccgtccc ccccggcgag accgcggcgt cgttcctgag gaagatgacc
     1141 gaggtggggg cgcgcgaacg gtaccgcccc taccacgacc gcgcccgcgc gcaggtggcc
     1201 cgcgagttga acctgatcga gaagctcggc ctcgccggct acttcctcat cgtctgggac
     1261 atcgtgaact tctgccgcca gcagggaatc ctggtgcagg gacgcgggtc ggccgccaac
     1321 agcgccgtct gctacagcct cgggatcacg gcggtggatc cggtggggat ggacctgctg
     1381 ttcgaacgct tcctctctga agagcgcggc gagtggcccg acatcgacct cgacctgccg
     1441 agcggcgacc gccgcgagcg cgtcatccag cacgtctacg agaagtacgg cgagcacggc
     1501 gcggccatga ccgccaacgt catcacgtac cgcgggcgca gcgcggcgcg cgaggtgggg
     1561 aaggtggtcg gcctcgacgc ggccaccatc gaccggctct ccaaggtgct ccaccggttc
     1621 gagtaccacg acgacgggga gacgatcgcg aagcacctcg gcgagatcgg cgtcgacccc
     1681 accgatcacc gcatgagggt gttcgcgggg ctgtggcagc ggatccagga cctgccgcgg
     1741 cacctcgggc agcactcggg cgggatggtg atctgccagg ggcgcctcga cgcggtcgtc
     1801 cccctcgaga acgccagcat gcccggccgc gtcgtcgtgc agtgggacaa ggacgactgc
     1861 gccgacatgg ggatcgtgaa gatcgacctg ctcggcctcg ggatgatggc ggtgctgcag
     1921 gactcgctgg ccgcgatcaa cgcccgccgc ccgtcggcgg cgcagccgct cgggttgtgg
     1981 aacctgccgc ccgacgaccc ggaggtctac cggatgctgc gcgccgccga cacgatcggc
     2041 gtgttccagg tggagtcgcg cgcgcagatg gcgactctgc cgcggctgaa gcccgagcgc
     2101 ttctacgacc tggtcgtcga ggtggccatc atccggccgg ggccgatcgt cgggaacatg
     2161 gtgcacccct acctgaaccg ccgcgccggc cgcgagacgg tgacctaccc gcacccgtcg
     2221 ctcgagccga tcctcgcgcg cacgctcggc gtgccgctct tccaggagca gctgctgcgg
     2281 atcgcgatgg tggcggccgg gttttccggc ggccaggccg aggagctgcg gcgggcgttc
     2341 gggttcaagc ggtccgagcg ccggatgaag cagatcgagg tgcagctgcg cgaggggatg
     2401 gcgcgcaagg ggattaccgg ccaggcagcc gaggacatcg tcaaggcgat cgcgtcgttc
     2461 gcgctctacg gtttccccga gtcgcactcg gccagcttcg cgctgctggt ctatgcgagc
     2521 gcgtacctga aggcgcacta cccggcggcg ttctacgcgg cgctgctcaa caactggccg
     2581 atggggttct accacccggc cacgctggtc aaggacgcgc agcggcgcgg cgtccgcttc
     2641 gcgccagttg acgtgcagcg ctcgcactgg ccctgcacga tcgaggagga cggcgccatc
     2701 cgcctcgggt tctgctacgt gaagggtctg agaaggcagg tgggggaggc gattgaagca
     2761 gctgacagcc gacagcagac agcggacagc ctgtcacgcg ggccacgcaa gcccgagcgc
     2821 tgccccaagt gcgggtgcga cgacgaatcg atgctcgagg tgtcgcggca gggcgggcgg
     2881 ctggcctgct tctgcaacaa ctgcgcgcac gactggaagg tcgtgccgcc gcgcttccgc
     2941 tcggtggacg agctgatcgc gcgcaccggg atcaaccgcg aggagttgtc cacgctggcc
     3001 gaggccgggg ccctcgcctc gctcggtttc acgcgccgcg aggcgctctg gcaggtggag
     3061 cgggcgatca ggccggccgg agagctgttt caggattcgg gattcgggat tcgggattca
     3121 ggattcggga ttcagggatc agggatcagg gatcagagat tggggatcgg ggaacaggaa
     3181 gaggacagct cgcccttgcc cgagatgacg ccggtcgagc ggctggtggc cgattacgag
     3241 gggacggggc tgaccattgg gccgcacccg atggcgatgc ggcgcgagga gatggagatg
     3301 cgtggcgcgc tgcgggccat cgacctcgca aagcagcgcc acggccggcg cgtgcgcgtc
     3361 gccggcgcgg tcatcacgcg ccagcgcccg ggcaccgcga agggcttcgt cttcctcacg
     3421 ctcgaggacg aaaccggtat cgccaacatc atcgtccggc ccgacctgtt cgcccgctcg
     3481 cgcctgacgg tggtcggcga gccgttcctg ctggtcgaag ggatcctgca gaaccaggaa
     3541 ggggtggtct cagtgcgcgc cgagcggctc gacgggatgg gcggggcgaa ggtggacgtg
     3601 gaggcgaggg acttctattg agagggtcac ccctacggtg tcagggtcag cgtgtgcgcc
     3661 gtcttccccg gcagcagcgg cgtcgggtcg ccgttcaccc aggcggcgtg gccgtcgcgg
     3721 tagtgcggtg acaaggggtg gccgctctgg cctcccggca tgtggaagat gccgtcctgt
     3781 tcgtgccccg gcgacacgac gagacgctcg gacggcatca cgccgtccac gcgctgcagc
     3841 ggcatgtcgg cgccgccgcg cagcggcatc tcctccatgt cgaaccagcg ggccagcaca
     3901 ccgagtcccg cgctcagcgg gtggcgaatc cggctgtcga tcgtgcccca gcgaagctcg
     3961 tccaacttcg ccccgtccga ggtcagttcc ccgatggcgc cgtccacggc cgccaacagc
     4021 tgttcgtccc agctttcgaa gcgcgccgag agcaggtgcc gcgggcgctc gctgacgagc
     4081 cgccacagcg gcccctcgat gcgggtgggg aaggggggca gcggcagtgc cgggtcggct
     4141 ggccgcaccc agtcgaacga gggcatcacg gatcgggccg gggcggtcag cacgccgaag
     4201 acctgctcgg ccagcacacg ccgccagcac tggaccagcg tgtatgccac cgagtcggac
     4261 gcgcggccgg tccacgtcga cgcgaccagg cggcggtact cctcgcgcgc ggcgctgccg
     4321 cgagcctccg gcctggagag ggtctcgagc aggagggtgc gccatcgctc gaggaacagg
     4381 ggccgctcgt ccatctggac gtgcagcagg tcgcgctcgg tggcctttga aagggcgagc
     4441 agcgcgtccc tgatctggcg cgcccgcgcc gacgcgtcca cggccgccgc cgaatacact
     4501 tcgggcgagg cgccgtagac gacctggttg ttggcagtcc agatccggcc gccgggcgga
     4561 tcgatgatgc gcgggcgggt ttcgggcggc agcagaccgt cccaccgtcg cgagccgtcg
     4621 gcccacgacg
//
LOCUS       DAOPQX010000301         4629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20204_length_4629_cov_4.509401, whole genome shotgun sequence.
ACCESSION   DAOPQX010000301 DAOPQX010000000
VERSION     DAOPQX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20204_length_4629_cov_4.509401"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1285
                     /locus_tag="PKK95_13740"
     CDS             194..1285
                     /locus_tag="PKK95_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19330.1"
                     /translation="MPSCRLSRPALAVVLVTFLAAAGPGRAEAQPAGPEQKAEPPAPA
                     WKISGLVFGDFYLFAQDHDERFDGQSGFWLRRGYLTYDHTLAPTLATRFRLEVNSNGK
                     MAGGSLTPFVKDAYLRWSYRKGHQAFVGLQPTPTLETIDGVWGLRYVERVAADLYRFD
                     TTRDLGVGVTGSLNAGGTVRYAAQIGNDSGTASETDGKMAIRLAARYDTNPGLFVEGS
                     YWHADRAGGADREALQVFAAYRHARGRAAVNLVRHERDAAPGSLPAVETSVVSGFGVL
                     EVRPGRLSVFARLDRVFDPLPDGGIDYLPISTASPFTFGLFGVEYRPAPSVWLAPNLE
                     FVRYGTPRDGAAARPRNDVVVRASFHWAW"
     gene            complement(1331..2602)
                     /locus_tag="PKK95_13745"
     CDS             complement(1331..2602)
                     /locus_tag="PKK95_13745"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002772.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOC19331.1"
                     /translation="MKNVLALILGGGRGTRLFPLTHMRSKPAVPIGGKYRLIDVPVSN
                     CLHAEIRRIYVLTQFNSASLNRHIAETYRMDLFSRGFVEILAAEQTPDSSSWFQGTAD
                     AVRQAQRHFIYHDADYYLILAGDHLYRMDYAAMIDEHVERRADITIAVQPVTPADATA
                     MGILRFDHDGQIAAFEEKPNVERLRQIGSSVPPGSRFPLPDSDKPFVASMGIYVFTRD
                     VLLEMLERHRGVDFGHEIIPAALGSCRVNAHVFRGYWQDVGTVSSFYDANIMLTRPGA
                     PFSFYDPRRPVFTRPRWLPASRVDGCHLEQAVVAEGCWIDRSAISQSVIGIRSRVGRD
                     CRVSRSVLLGADFYEHDDDAPSGNGAPDLGIGAGSVLDRVIVDKNARIGEGVRLVNEA
                     GVDQADGDGFYIRNGIIIVPKWGVVPAGTVA"
     gene            2729..3346
                     /locus_tag="PKK95_13750"
     CDS             2729..3346
                     /locus_tag="PKK95_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOC19332.1"
                     /translation="MAFRLIPREEKFYDDFVAIADQLTKGAALLEGLLSTDPPQVDKA
                     DEIKEIEHQCDFLSHAIYQRLHRTFVTPLDREDIHALAGALDDVMDAMDASAGYVRLY
                     KVTEIRFGARELARIIVEMAKTVRLAIDGVGPRKGVAPHIVEINRLENEADRMHFEAV
                     RRLFDEEKDPILLFKWKEILDRLEDATDRCEDVADVIEGIVLKNG"
     gene            3355..4353
                     /locus_tag="PKK95_13755"
     CDS             3355..4353
                     /locus_tag="PKK95_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOC19333.1"
                     /translation="MELAVVALLIVVALIFDFINGFHDAANSIATVVSTRVLSPGKAV
                     VWAAFFNFVAVFSFGTAVARTVGSGLIDIQQVTFAVIFAGLMGAIIWDLITWYFGLPT
                     SSSHALIGGYAGAAVARAGLGALIPSGWTKTLAFIVISPLVGLIAGLFLMVAIYWLLQ
                     RVPPAKVDHWFRRLQLVSAAAFSLNHGGNDAQKTMGIIAGVLFTAGYLKGEFYIPVWV
                     VVAAGAAIGLGTLTGGWRIIHTMGSRITKLQPVHGFAAETGAAGAVLLATHLGVPVST
                     THAITGAIVGVGSTRRLSAVRWGVAGQIVWAWVLTIPAAAGIGALVYVLLGVLDLR"
     gene            complement(4379..>4629)
                     /locus_tag="PKK95_13760"
     CDS             complement(4379..>4629)
                     /locus_tag="PKK95_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19334.1"
                     /translation="AASLGHAAISLAAVGLLVGALFGCAAARQPLGERGAGRLYAADV
                     IGGAVGSLAATVLLVPLAGLEATALLVAAVALATLTIP"
BASE COUNT          688 a         1609 c         1586 g          746 t
ORIGIN      
        1 gcagaacgga cgccaccggg gatcctgcgg ccgcttgcga cagtagacgc agaggggctc
       61 gggcacaccc ggattctaac agaccgccgc cacgccaccg gccagatgct cgggattgca
      121 gccgcccggg atgcgcaccc tcttgcggat cgcgtgatcg tcccggcgca ttctcggata
      181 tcgttgcggg tgcatgcctt cctgcaggct ctcccggccc gcccttgcgg tggttctcgt
      241 gacgttcctc gccgcagccg gacccgggcg agctgaggcg cagcctgccg ggcccgagca
      301 gaaggccgag ccgccggctc ccgcctggaa gatcagcggc ctggtgttcg gcgacttcta
      361 tcttttcgcg caggaccacg acgagaggtt cgacggccag tccgggttct ggcttcgccg
      421 cggctacctg acctacgacc acaccctggc tcccaccctc gcaacccgtt tccgtctcga
      481 ggtgaacagc aacggcaaga tggccggagg gagcctgact cccttcgtga aggacgccta
      541 cctgcgctgg agctaccgca agggtcacca ggcgttcgtc ggcctccagc cgactccgac
      601 gctcgaaacg atcgacggcg tctggggtct tcggtacgtc gagagggtgg cggcagatct
      661 ctaccggttc gacaccacga gggatctcgg cgtgggcgtc accggctccc tgaacgcggg
      721 cgggaccgtc cggtacgcgg cgcagatcgg gaacgactcc ggcaccgcct ccgaaaccga
      781 tgggaagatg gcgattcgcc tggccgctcg ctacgacacg aacccgggcc tgttcgtcga
      841 gggctcgtac tggcacgccg accgggccgg tggagccgac cgcgaggcgc tgcaggtgtt
      901 cgccgcttac cgccacgcca gggggcgcgc cgcggtcaac ctggtgcggc acgagcgcga
      961 cgccgctccg ggcagcctgc cggctgtcga gacgagcgtc gtctcagggt ttggcgtgct
     1021 ggaggtccgc cccggccgcc tgtcggtgtt cgcacgcctc gaccgggtgt tcgacccgct
     1081 gccggacggc ggcatcgact acctgccgat cagcaccgcc tcgcccttca ccttcggcct
     1141 gttcggcgtg gagtaccgcc ccgctccctc cgtctggctc gcgccgaacc tcgagttcgt
     1201 gcggtacggc acgccgcgag acggtgccgc cgcgcggccc cggaacgacg tggtcgtgcg
     1261 ggccagcttc cactgggcct ggtagcccgc gatcgcgaat cgcatcctcg gcgggcgggt
     1321 cagggcggcg ttacgccacc gtgccggcgg gcaccacacc ccacttcggc acgatgatga
     1381 tcccgttccg gatgtagaac ccgtcaccgt cggcctggtc aacgcccgcc tcgttgacca
     1441 ggcgaacccc ctcgccgatg cgggcgttct tgtcgacgat gacccggtcg agcaccgagc
     1501 cggcgccaat gcccaggtcg ggcgccccgt tgccgctcgg cgcatcgtcg tcgtgctcgt
     1561 agaagtcggc ccccagcagc accgagcgcg agacgcggca gtcgcggccg acccggctgc
     1621 ggatgccgat caccgactgg ctgatggccg accggtcgat ccagcagccc tccgccacca
     1681 ccgcctgctc caggtggcac ccgtccacgc gcgaggcggg cagccaccgc ggccgcgtga
     1741 acaccgggcg gcgggggtcg tagaaggaga acggggcgcc gggccgcgtc agcatgatgt
     1801 tcgcgtcgta gaacgacgag acggtcccga cgtcctgcca gtatccgcgg aacacgtgcg
     1861 cgttgacccg gcagctgccg agcgcggcgg ggatgatctc gtggccgaag tccaccccgc
     1921 ggtgacgctc cagcatttcg agcagcacgt cgcgcgtgaa gacgtagatc cccatcgagg
     1981 cgacgaacgg cttgtcgctg tccggcagag ggaaccggga gccggggggg acgctcgacc
     2041 cgatctgtcg caggcgctcg acgtttggct tctcctcgaa cgcggcgatc tggccgtcgt
     2101 ggtcgaagcg gaggattccc atggccgtgg cgtcagccgg ggtcaccggc tgcaccgcga
     2161 tcgtgatgtc ggcgcggcgc tcgacgtgtt cgtcgatcat cgccgcgtag tccatccggt
     2221 acaggtggtc gccggccagt atcaggtagt agtcggcatc gtgatagatg aagtgccgct
     2281 gtgcctggcg caccgcgtcg gcggtcccct ggaaccagct cgagctgtcg ggtgtctgtt
     2341 ccgcggcgag gatctcgacg aagccgcgcg agaacaggtc cattcggtag gtttcggcga
     2401 tgtgccggtt gagcgacgcc gagttgaact gggtcaggac gtagatgcgc ctgatctccg
     2461 cgtgcaggca gttgctcacc ggcacgtcga tgagccggta tttgccgccg atcggcacgg
     2521 cgggcttcga cctcatgtgc gtgagcggga agaggcgggt cccccggccg ccgccgagga
     2581 tgagtgcgag gacgttcttc atggtggtct tcggggcgat cgcccgccca ttccgtgtcg
     2641 cccggcacgt cgggctgccc gagatcctac agcgaccggg gatctgatac aattcctcgg
     2701 cttttgggcc ctgaagggag cgactgccgt ggcattccgt ctgatccccc gcgaagagaa
     2761 attctacgac gacttcgtcg ctatcgccga ccagctcacc aagggcgccg cgctgctcga
     2821 gggtttgctg tcaaccgacc ccccgcaagt cgacaaggcc gacgagatca aggagatcga
     2881 gcaccagtgc gatttcctca gccacgccat ctaccagcgg ctgcaccgga ctttcgtgac
     2941 gccgctcgac cgtgaggaca tccacgcgct cgccggcgcg ctcgacgacg tcatggacgc
     3001 gatggacgcg agcgccggct acgtcaggct ctacaaggtg acggagatcc ggttcggcgc
     3061 gcgcgagctg gcgcgcatca tcgtcgagat ggccaagacc gtccgcctgg ccatcgacgg
     3121 tgtcgggccg cgcaaggggg tggcgcctca catcgtcgag atcaaccgcc tggagaacga
     3181 ggccgaccgg atgcacttcg aggccgtgcg gcggctgttc gacgaggaga aggacccgat
     3241 cctgctgttc aagtggaagg agatcctcga ccggctcgag gacgcgacgg accgctgcga
     3301 ggacgtggcc gacgtcatcg aaggcatcgt cctcaagaac ggctgagggt ccgcatggaa
     3361 ctcgccgtcg tcgcactcct gatcgtcgtc gccctcatct tcgacttcat caacggcttc
     3421 cacgacgccg ccaactccat cgccaccgtc gtctcgacgc gcgtcctgtc gccgggcaag
     3481 gcggtcgtct gggctgcctt cttcaacttc gttgccgttt tctccttcgg caccgccgtc
     3541 gccaggaccg tcgggtcggg gctcatcgac atccagcagg tgaccttcgc cgtcatcttc
     3601 gccggcctga tgggagccat catctgggac ctgatcacct ggtatttcgg gctcccgacc
     3661 agctcctcgc acgccctgat cgggggctac gccggggccg ccgtcgcgcg ggcgggtctc
     3721 ggagcgctca tcccatccgg ctggacgaag acgctcgcgt tcatcgtgat ttcgccgctg
     3781 gtcggcctca tcgccggcct gttcctgatg gttgccatct actggctgct gcagcgagtc
     3841 ccgccggcga aggtggatca ctggttccgc cggctgcagc tcgtgtcggc cgctgccttc
     3901 agcctgaacc acgggggcaa cgacgcgcag aagacgatgg ggatcattgc cggcgtcctc
     3961 ttcaccgccg gatacctgaa gggtgagttc tacatcccag tgtgggtcgt ggtggccgcg
     4021 ggcgccgcga tcggactggg gacgctgacc ggcgggtggc ggatcatcca caccatggga
     4081 tcgcgaatca ccaagctgca gccggtgcac ggcttcgccg ccgaaactgg ggcggccggc
     4141 gcggtgctcc ttgccacgca tctcggtgtc ccggtcagca ccacgcacgc catcaccgga
     4201 gcgatcgtcg gtgtgggatc cacgcgccgg ctctcggccg tccgctgggg cgtcgccggg
     4261 cagattgtct gggcgtgggt gctgacgatt ccggcggccg ccggcatcgg cgcgctggtc
     4321 tacgtgctgc tcggggtgct cgacctccgc tgagcgcgtc ctgccgccgg gcgggacgtc
     4381 aggggatggt cagcgtggcg agcgcaaccg cggcgaccag cagggcagtc gcctccagcc
     4441 cggccagcgg gaccaggagc accgtggccg ccagcgaccc gaccgctccc ccgatgacgt
     4501 cggcggcgta caggcggccg gcaccgcgct cgccgagcgg ctggcgcgcc gcggcgcagc
     4561 cgaacaacgc ccccaccagc aggccgacgg ccgccaggga gatggcggcg tgcccgaggc
     4621 tcgcggcca
//
LOCUS       DAOPQX010000302         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20225_length_4626_cov_4.084008, whole genome shotgun sequence.
ACCESSION   DAOPQX010000302 DAOPQX010000000
VERSION     DAOPQX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20225_length_4626_cov_4.084008"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PKK95_13765"
     CDS             <1..950
                     /locus_tag="PKK95_13765"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC19335.1"
                     /translation="DQRLAEAGHAGEAYGQSVAYRSGGAVAAARLGAVASLVRSVGGA
                     EYRLQHTGGTSYADGVPRIPAGALAAEDAALVERLSKRGPVRLRLVLTPRTLPDATDH
                     NVIGDLTGSERPDEVVIVSGHLDSWDLGTGAIDDASGVAVAMEVANLVKQAGLRPRRT
                     IRVVAWAAEEPGLLGSRRYVKEFASKLRQHAGAIEMDLGAAHPIGIEIGGAPSIAQVL
                     APVAAILRGSGAGVLRTGELAGSDIIALGIDGVPTFAPLQDTRRYFDYHHTPADTLDK
                     VDPEELRENAAVIAVLAYALANLPEMLPHTPRPRPAWLE"
     gene            complement(955..1584)
                     /locus_tag="PKK95_13770"
     CDS             complement(955..1584)
                     /locus_tag="PKK95_13770"
                     /inference="COORDINATES: protein motif:HMM:NF021512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HOC19336.1"
                     /translation="MSEPVTTATFSCTAFMGSRRIASGPLPEVAVAVKELVDRDQISP
                     VLVFDDETSEVVDLDLRGTAEQVRRHAEGLAASRGVRAPEPGQDAAEPAGPRGPGRPR
                     LGVVAREVTLLPRHWQWLGSQPGGASAALRRLVDEARRTHAGRDRVRRAQEAAYRFMS
                     AMAGDEPGFEEATRALFAGNLAVFDALVEPWPPDVRDHARKLAAPALLP"
     gene            complement(1680..4526)
                     /locus_tag="PKK95_13775"
     CDS             complement(1680..4526)
                     /locus_tag="PKK95_13775"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOC19337.1"
                     /translation="MFTQVLAAALRAIFVVPALAVLLLPAAAQVGPAAPATEVQFLSG
                     HGPEDAVEWEFFCTAGRRSRVWTTIPVPSCWEQHGFGGYNYGNEINADGPSANEQGLY
                     RRSFTIPSNWRGRRVRIVFDGAMTDATVWINGQQAGPTHQGAFYRFHYDITPLARFGE
                     ANLLEVTVAKVSGIRSVNSAERAADYWVFGGLFRPVWLEARPEVSIERAAIDARHDGR
                     LSIDVFLAGDAGGSVVTARVADAEGRPVGEPLRSSAAGADGKVTVRGRLEGVRPWTAE
                     TPDLYRVELTLAGAEGRPLHVATERFGFRTVEVREGDGVYLNGTRIVLKGVDRHAFWP
                     ETGRTVSRAQSELDVRLMKEANLNAVRLSHYPPDEHFLEACDELGLYVLDELAGWQKA
                     YGTEVGARLIGELVRRDVNHPSILLWDNGNEGGWNEKNDAEFARWDPQNRAVLHPFAT
                     SGGINTDHYESYESTVKLSAGPTIFMPTEFLHGLYDGGIGAGLRDYWDVMGRSRTVAG
                     GFLWVWADEGIARTDRNGRIDTVGNQAPDGMVGPHREKEGSYFTVKEIWSPVQATLPA
                     DASGALPRDWDGTVAVENGYDFTSLDRCRFEWQLLRFGAPGDARTARVVVARGTLPGP
                     PLAPRTSGVVKLPLPRSWHEADALHVTAHDPAGEALWTWSAPRLGARAPRSGQAGGMP
                     VAVREEAGAIVVAGRAGELRFDRATGRLVEWQRDGLAAPLGPGPFARAFVRKQRTHAA
                     VEDVAKLELLTSRLDGKAVVVEARYEGILKRVSWRIEPASDAVQLDYELAYDGAIDLL
                     GVGFDAPPGGFVSKRWLGRGPYRVYRNRMEGGLFDLHQVAFNDPVPGQSFTYPEFKGY
                     FRDWQWLQLEAPAATLTIDNRSGVPFFGLHGPLDGQPPMLAFPDTGVALLHVIPAQGT
                     KFDTPDRLGPQSQTPGGIGVRQGSVTVRFDPAKE"
BASE COUNT          676 a         1695 c         1597 g          658 t
ORIGIN      
        1 tcgaccagcg gctcgccgag gccggccacg caggggaggc ctacggccag tcggtggctt
       61 accggagcgg cggggcagtc gccgccgcgc ggctgggcgc cgttgcctcg ctggttcgct
      121 cggtcggcgg cgccgagtac cggctgcagc acacgggcgg cacctcgtac gccgatggcg
      181 tcccccggat cccggcaggc gcgctcgccg ccgaggacgc cgcactcgtc gagcggctgt
      241 cgaagcgcgg accggtgcgc ctgcgcctcg tcctcacgcc gcggacgctg cccgacgcga
      301 ccgaccacaa cgtcatcggc gacctgacag ggagcgagcg tcccgacgag gtggtgatcg
      361 tctcgggcca cctcgattcg tgggacctcg ggacgggcgc catcgacgat gcctcaggcg
      421 tggcggtggc gatggaggtg gccaacctgg tgaagcaggc cgggctgcgg cccaggcgga
      481 cgattcgtgt ggtggcctgg gcggccgagg agcccgggct gctcgggtcg cgccgctacg
      541 tgaaggagtt cgcctcaaag cttcggcagc acgccggcgc gatcgagatg gacctgggcg
      601 cggcgcaccc gatcggcatc gagatcgggg gcgccccgtc catcgcccag gtgctggcgc
      661 cggtggcggc catcctccgg ggctcgggcg cgggagtgct gcgcaccggc gagctggcag
      721 gatcggacat cattgcgctc gggatcgacg gcgtgccgac cttcgcgccg ctgcaggaca
      781 cgcggcgcta cttcgactac caccacacgc cggccgacac gctggacaag gtcgatcccg
      841 aggaactgcg cgagaacgcc gcggtcatcg ccgtcctggc gtatgccctc gccaacctgc
      901 ccgagatgct cccgcacacg ccgcggccgc gtcccgcctg gctggagtag gggctcaggg
      961 caggagcgcc ggggccgcga gcttgcgcgc atggtcgcgc acgtcgggcg gccacggctc
     1021 gaccagcgcg tcgaacacgg caaggttgcc cgcaaagagc gcgcgcgtcg cctcctcgaa
     1081 ccccggctcg tcgccggcca tcgccgacat gaaccggtac gccgcttcct gcgcacggcg
     1141 aacgcggtcg cggccggcgt gcgtgcggcg tgcttcgtcc accaggcgtc tcaaagctgc
     1201 cgacgcacct cccggctggg agccgagcca ctgccagtgg cgcggcagca gcgtgacctc
     1261 gcgcgcgacg acgccaagac gcgggcggcc ggggccgcgc ggcccggccg gctcggccgc
     1321 gtcctgcccc ggctccggcg cgcggacgcc gcgcgacgcc gccaggccct ccgcgtggcg
     1381 ccgcacctgc tcggcggtgc ccctgaggtc gagatcgacg acctcgctcg tctcgtcgtc
     1441 gaacaccagg accggcgaga tctggtctcg atcgaccagc tccttcaccg cgacggccac
     1501 ctcggggagg ggacccgaag cgatgcgccg gctgcccatg aaagctgtgc aggagaacgt
     1561 ggcggttgtt acaggctcag acatggccat atgtatccag gttcaaacgt agggtatgga
     1621 atattaccca gatacaatat acatgtcaac agcgcgccca cctctctact cctttgcctc
     1681 tactccttcg ccgggtcaaa cctcacggtg acgcttccct ggcgcacgcc aatcccgcct
     1741 ggcgtctgcg actgcgggcc aagccgatcc ggggtatcga acttggtccc ctgcgccggg
     1801 atgacgtgca gcaacgccac gccggtatcg gggaatgcca gcatcggcgg ctggccgtcg
     1861 aggggcccgt gcagcccgaa gaacggcact cccgaacggt tgtcgatggt gagcgtggcg
     1921 gccggcgcct cgagctggag ccattgccag tcgcggaagt agcctttgaa ctcggggtag
     1981 gtgaacgact ggcccggcac gggatcgttg aaggcaacct ggtggaggtc gaagagcccg
     2041 ccttccatcc ggttgcggta gacgcggtag ggaccgcgtc cgagccagcg cttcgacacg
     2101 aagccgcctg gcggggcgtc gaagccgaca ccgagcaggt cgatcgctcc gtcgtacgcc
     2161 agctcgtaat ccagctgcac cgcgtcgctc gcgggctcga tccgccacga cacccgtttg
     2221 aggattccct cgtaccgcgc ctcgacaacg actgccttgc cgtcgaggcg cgaggtcagc
     2281 aactccagct tcgccacgtc ctcgacggcg gcatgggtcc gttgcttccg gacgaaggcc
     2341 ctggcaaagg ggccaggccc gagcggggcc gccaggccgt cgcgttgcca ctcgacgagg
     2401 cggccggtcg cccgatcgaa ccgcagttcc ccggcacggc ccgcgacgac gatggcgccc
     2461 gcctcctcgc gcacggcaac aggcatcccg ccggcttgcc ccgagcgagg cgccctggcg
     2521 ccgagtcgag gggccgacca cgtccacaac gcctcgccgg ccgggtcgtg cgccgtcacg
     2581 tgcaaggcgt ccgcctcgtg ccacgaacgc ggcagcggca gcttgacgac gcccgaggtt
     2641 cgcggggcga gcggcggccc cggaagggtt ccccgtgcca ccaccacccg cgccgtcctc
     2701 gcgtcaccgg gagccccgaa gcgcagcagc tgccactcga agcggcagcg atcgaggctc
     2761 gtgaagtcgt agccgttctc caccgccacg gtgccatccc agtcgcgagg cagggcgccg
     2821 gaagcatcgg ccggcagcgt ggcctggacc ggcgaccaga tttccttcac cgtgaagtag
     2881 ctgccttcct tctcccggtg cggccccacc atgccgtcgg gcgcctggtt gcccaccgtg
     2941 tcgatgcggc cgttgcggtc ggtgcgggcg atgccctcgt cggcccagac ccagaggaag
     3001 ccgcccgcca ccgtgcggct tcgccccatc acgtcccagt agtcgcggag ccccgcgcca
     3061 atcccgccgt cgtagaggcc gtgcaggaac tcggtcggca tgaagatggt cgggccggcg
     3121 ctcagcttca ccgtgctctc gtagctctcg tagtgatccg tgttgatgcc gccgctggtg
     3181 gcgaacgggt gcaggacggc gcggttctgc gggtcccagc gggcgaactc cgcgtcgttc
     3241 ttctcgttcc agccgccctc gttgccgttg tcccacagca ggatgctggg gtggttgacg
     3301 tcgcggcgaa ccagttcgcc gatcagccgg gcgccgacct cggtgccgta ggccttttgc
     3361 cacccagcca gctcgtcgag cacgtacagc cccagctcgt cgcatgcctc caggaagtgt
     3421 tcatcgggcg ggtagtgcga caggcgcacc gcgttgaggt tcgcctcctt catcaggcgg
     3481 acgtccagct cgctctgcgc gcgcgacacc gtgcggcccg tttcgggcca gaaggcgtgg
     3541 cgatcgacgc ccttcaacac gatcctggtg ccgttcaggt agacgccgtc gccctcgcgc
     3601 acctcgacgg tgcggaagcc gaagcgctcg gtggcgacat gcagcggccg gccctcggcg
     3661 ccggccagcg tcagctccac gcgatagagg tccggcgtct cggcggtcca cggcctgaca
     3721 ccttccagcc gtccccgcac ggtcaccttg ccgtcggcgc ccgccgcgga agaacggagc
     3781 ggctcgccca ccggccttcc ctccgcgtcc gcgacccgcg cggtcacgac cgagccaccc
     3841 gcgtcgccgg ccaggaacac gtcgatcgag agccggccat cgtgacgggc gtcgattgcc
     3901 gcccgctcga tcgacacctc gggccgcgcc tcgagccaca cgggccggaa caacccgccg
     3961 aacacccagt agtcggccgc ccgctcggcg ctattgacgc tgcggatccc cgacaccttc
     4021 gcgacggtca cctcgaggag gttggcctca ccgaacctgg cgagcggcgt gatgtcgtaa
     4081 tggaaccggt agaaggcccc ctggtgcgtc ggccctgcct gctgcccgtt gatccacacg
     4141 gtggcgtcgg tcatcgcgcc gtcgaagacg atgcgcactc gccgcccgcg ccagttggac
     4201 ggaatcgtga acgatctccg gtagagcccc tgctcgttgg cggacggccc gtcggcgttg
     4261 atctcgttcc cgtagttgta gccgccaaac ccgtgctgct cccagcacga cggcacgggg
     4321 atggtggtcc acacgcggct gcgccggccg gcagtgcaga agaactccca ctcgacggcg
     4381 tcctcgggcc cgtgccccga gaggaactga acctccgtcg ccggcgcagc ggggccgacc
     4441 tgcgccgcag ccggcagcag cagcacggcg agggccggca cgacgaagat ggcgcgaagg
     4501 gccgcggcaa ggacctgcgt gaacatcgtg agcctccgag gggcaggtgc ccgcccaaga
     4561 tacctcaccg gcggcgcttc ggttcccggc acagccgggc tggccgtcgt cgcctgccgg
     4621 gcgctt
//
LOCUS       DAOPQX010000303         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20251_length_4622_cov_4.479746, whole genome shotgun sequence.
ACCESSION   DAOPQX010000303 DAOPQX010000000
VERSION     DAOPQX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20251_length_4622_cov_4.479746"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1040
                     /gene="msrP"
                     /locus_tag="PKK95_13780"
     CDS             96..1040
                     /gene="msrP"
                     /locus_tag="PKK95_13780"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HOC19338.1"
                     /translation="MIIRTRREIPSSQITDERLYLRRREFITLAGAGVATAAAACAEG
                     AQAQRPPALAGVRPGPFGTSEARTPYEDVTTYNNFYEFGADKGDPARHAGGLRTRPWT
                     VRIDGECQKPADYQVEDLIKPNALEERVYRLRCVEGWSMVIPWVGFPLARVIARAEPG
                     TRARFVEFTTLLDPRQMPGQRDPVLEWPYVEGLRLDEATHPLAILAVGLYGATLPNQN
                     GAPLRLVVPWKYGFKSIKSIVRIRFVEKQPVTAWQRQNPREYGFYSNVNPEVDHPRWS
                     QARERRIGEFFKRRTLMFNGYGDEVASLYAGMDLRRNF"
     gene            1051..1692
                     /locus_tag="PKK95_13785"
     CDS             1051..1692
                     /locus_tag="PKK95_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HOC19339.1"
                     /translation="MPRAHRRVIKPLVFAAALLPLGWLVRRGLSGTLGANPLETITHE
                     TGLWTLRFLLITLAVTPARRLLRLNALIAYRRMLGLFAFAYGCLHLITYLWFDQFFAW
                     GEIVRDIAKRPFITAGMAGFAAMVPLALTSTAAMIRRLGGRRWRALHRLVYAAAAAGV
                     AHYWWLVKADTRSPRAYALVLAVLLLARLLPAGLTGSARPAAGGPRGRPSATR"
     gene            complement(1631..2290)
                     /locus_tag="PKK95_13790"
     CDS             complement(1631..2290)
                     /locus_tag="PKK95_13790"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOC19340.1"
                     /translation="MESVLGWVQEFFRIVYNVPELIRLVGLVGLIAIVFAETGLLVGF
                     FLPGDSLLVTAGLFAARGDLDVVWLIVTLMAAAVLGDATGYYIGRRAGQALYSRPNSF
                     FFRRQHLIRTHEFYERHGGKTIVIARFVPIIRTFAPVVAGAAQMTYSHFATYNIVGGI
                     LWVNSMVLGGYYLGRLVPDIEHRLHLVVAVVIFLSLLPAIIAWLKAYLSGRRRQVEPS
                     R"
     gene            2488..3135
                     /locus_tag="PKK95_13795"
     CDS             2488..3135
                     /locus_tag="PKK95_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19341.1"
                     /translation="MDVRTVAPAGQQADVVAMADEVVRTFLHVRDAFVRLDPAPLDEA
                     ARLARLLHRQEQALIHRMVQAADGSSAGAAGLDEEVIFVPMHLERVADNIELLGASVG
                     RMIRDGILFTDRATGEIRRLFATVLELLEGLRDALRTGNRTLVRYIRDAGHAAEAQAN
                     EFALFHEQRLIEGVCEPKASSVYLAMLDQLRGIEWHARQVAEKLGTGSLPDEPYA"
     gene            complement(3140..>4622)
                     /locus_tag="PKK95_13800"
     CDS             complement(3140..>4622)
                     /locus_tag="PKK95_13800"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOC19342.1"
                     /translation="VQTVIPFGFAVVALRAWWQAGVPVGVGDQPAPPLPARAAWERRL
                     IVAAVVAATVSLAFAPEAVAPSVRWPLIVLLLAATVMGAPIFVTLGGAALVLFYADLV
                     PLAAVPSQTYGIVSSPTLPTIPLFTLAGVLLAEGGTPARLVRLFKALAGWLPGGTAVA
                     TILVCAFFTTFTGASGVTILALGGLLLPVLMRDVYSERFSLGAVTSAGSIGLLFPPSL
                     PVILYGISARTPVNEMFAAALIPGLVLVGLVSLYCLREGIARGGKWRAPDPAEIGRAA
                     WEAKWEMVLPVIVFVSLFGGFATTVEAAAITAAYAFFVKCVVHRDVSVTRELPRVVVE
                     SAALVGGVLIILGCALGLTGYLIDAEIPMQGAAWVKSAIHSRWLFLLTLNVFLLVVGC
                     LMDIFSAIFVVVPLILPIAAAFDVHPLHLGAIFLTNLELGYLTPPVGLNLFLSSLRFR
                     RSMFEVSRAALPFLGVQAAAVLLITYVPSLSVEGARWLMQLLG"
BASE COUNT          766 a         1616 c         1625 g          615 t
ORIGIN      
        1 ctggccgccg ccgtgcggcg gaccgcgtag ctgaccaagc cgccgcggtc gtcttctcag
       61 gcggtccctt cgcgtgcaga atgggaaggg gcgccatgat catcaggacc cgtcgcgaga
      121 tcccctcgtc gcagatcacc gacgagcggt tgtacctccg gaggcgtgag ttcatcacgc
      181 tcgccggtgc cggggtggcg accgccgccg cggcttgcgc cgagggggcc caggcgcaac
      241 ggccccctgc gctcgcgggc gtccggccgg ggccgttcgg caccagcgag gcgcgcaccc
      301 cctacgagga cgtcaccacc tacaacaact tctacgagtt cggcgccgac aagggggacc
      361 ctgcccgcca tgccggaggc ctccgcaccc gtccgtggac cgtccgcatc gacggcgagt
      421 gccagaagcc ggcggactac caggtagagg atctgatcaa gccgaacgcc ctggaggagc
      481 gggtgtaccg cctccggtgc gtggagggtt ggtcgatggt gatcccgtgg gtggggttcc
      541 cgcttgccag ggtgatcgcg cgggccgagc caggcacgcg cgccaggttc gtcgagttca
      601 cgacgctgct cgatccgcgt cagatgccgg ggcagcgcga ccccgtgctc gagtggccgt
      661 acgtcgaggg gttgcggctg gacgaggcga cccaccccct cgcgatcctg gccgtcggcc
      721 tctacggggc cacgctcccg aaccagaacg gcgcgccgct gcgcctcgtg gtcccctgga
      781 agtacgggtt caagagcatc aagtcgatcg tgcggattcg cttcgtggag aagcagccgg
      841 tgaccgcctg gcagcggcag aacccgcgcg agtacggctt ctactccaac gtcaaccctg
      901 aagtggatca cccccgctgg agccaggcgc gcgaacggcg gatcggcgag ttcttcaagc
      961 gccgcacgct gatgttcaac ggatacggcg acgaggtggc aagcctctac gcggggatgg
     1021 acctgcgcag gaacttctga gcgccacctc atgccgcgcg cgcaccgccg ggtgatcaag
     1081 ccgctggtct tcgccgccgc gctgctgccg ctgggctggc tcgtccgtcg cggcctctcc
     1141 ggcacactgg gcgccaaccc gctcgagacg atcacgcacg agacaggcct ctggacgctc
     1201 cggttcctcc tcatcaccct cgcggtcaca cccgcccggc gcttgctccg cctcaatgcg
     1261 ctcatcgcgt accggcggat gctggggctg ttcgccttcg cctacgggtg cctccacctg
     1321 atcacctacc tctggttcga ccagttcttc gcgtggggcg agatcgttcg cgacatcgcg
     1381 aagcggccgt tcatcaccgc cgggatggcg ggcttcgcgg cgatggtgcc gctggcgctc
     1441 acctcgacgg cggcgatgat caggcgcctg ggcggacggc ggtggcgcgc cctgcaccgc
     1501 ctggtctacg ccgcggcggc ggccggggtg gcgcactact ggtggctggt caaggccgac
     1561 acgcggtcgc cgcgcgccta cgccctcgtg ctggcggtgc tgctgctggc gcgcctgctg
     1621 ccggccggcc ttaccggctc ggctcgacct gccgccggcg gcccgagagg taggccttca
     1681 gccacgcgat gatcgcggga agcagcgaga ggaagatcac caccgccacc accaggtgga
     1741 ggcggtgctc gatgtccggg acgaggcgcc cgaggtagta gccgcccagc accatcgagt
     1801 tgacccacag gatcccgccc acgatgttgt aggtcgcgaa gtggctgtag gtcatctgcg
     1861 cggcgccggc gacgacgggc gcgaaggtgc ggatgatggg gacgaaccgc gcgatcacga
     1921 tggtctttcc gccgtgccgc tcgtagaact cgtgggtccg gatgaggtgc tgccgccgga
     1981 agaagaagga gttggggcgg ctgtagagcg cctgcccggc gcggcgcccg atgtagtagc
     2041 cggtcgcgtc ccccaggacg gcggccgcca tcagcgtcac gatcagccag acgacgtcga
     2101 gatcgccgcg cgcagcaaag aggcccgccg tcaccagcag cgagtcgccg ggcaggaaga
     2161 acccgacgag caggcccgtt tcggcgaaca cgatcgcgat gaggccgacg aggccgacca
     2221 ggcggatcag ctcgggcacg ttgtagacga tccggaagaa ctcctgcacc cagccgagga
     2281 ccgactccac gcgccactcc catccggcgg ccgttacgcc tccaccgcca aagctttgat
     2341 tataccggcc ccccggccct ccttttgagg gatacttgga atgtaccaac catcatcgac
     2401 gtcaagaccg gtaccaacgc gccgcgctgg agccgggcgc ggtctccgtc cgcggcgcgc
     2461 cagatccctt tgaggaagga ggaggctgtg gacgtgagaa cggtggcgcc cgctgggcag
     2521 caggccgacg tggtggccat ggcggacgag gtggtgcgga ccttcctgca cgtgcgcgat
     2581 gccttcgtcc ggctcgaccc cgcgccgctc gacgaggccg cgcggctcgc gcggctcctg
     2641 caccggcagg agcaggcgct catccaccgc atggtgcagg cggccgatgg cagcagtgcc
     2701 ggggccgccg gtctggacga ggaggtcatc ttcgttccca tgcacctcga gcgggtcgcc
     2761 gacaacatcg agctgctcgg cgcctcggtt gggcgcatga ttcgcgacgg catcctgttc
     2821 actgaccgag cgaccggcga gatccggcgg ctgttcgcga ccgtcctgga actgctcgag
     2881 gggctgcgtg acgcgctgcg caccggcaac cgtaccctcg tgcgctacat ccgcgacgcg
     2941 gggcatgccg ccgaggccca ggccaacgag ttcgccctct tccacgagca gcgcctcatc
     3001 gagggcgtct gcgagccgaa ggcctcatcc gtctacctcg cgatgctcga tcagctcagg
     3061 gggatcgagt ggcacgcgcg gcaggtcgcc gagaagctgg gcaccggctc gctgccggac
     3121 gaaccgtacg cctgatcgat cagcccagca gctgcatcag ccagcgcgcg ccctccacgc
     3181 tcagcgaggg cacgtaggtg atcagcagca cggccgcggc ctgcaccccg aggaagggca
     3241 gcgcggcgcg cgagacttcg aacatgctgc gccggaagcg cagcgacgac aggaacaggt
     3301 tcaggccgac cggcggggta aggtatccca gctccaggtt cgtgaggaag atcgccccca
     3361 ggtgcagggg gtgcacgtcg aaggccgccg cgatcgggag gatcagcggc accacgacga
     3421 agatcgccga gaagatgtcc atcaggcagc cgaccaccag caggaacacg ttcagcgtca
     3481 gcaggaagag ccagcgcgag tggatggccg acttcaccca ggccgccccc tgcatcggga
     3541 tttccgcgtc gatcaggtag ccggtcagcc cgagggcgca gccgaggatg atgagcactc
     3601 cgccgacgag cgccgccgat tccaccacca cgcgaggcag ttcgcgggta accgacacgt
     3661 cgcggtggac gacgcacttg acgaagaagg cgtaggcggc ggtgatggcc gccgcttcga
     3721 cggtggtggc gaacccgccg aacaggctca cgaagacgat gaccgggagg accatctccc
     3781 acttcgcctc ccatgcggcg cggccgatct cggccggatc cggtgcccgc cactttccgc
     3841 cgcgggcgat tccctcgcgg aggcagtaga gcgagacgag ccccaccagc accaggccgg
     3901 ggatcagcgc cgccgcgaac atctcgttca ccggggtccg cgccgagatg ccgtacagga
     3961 tcacgggcag gctcgggggg aacaggaggc cgatgcttcc cgcggaggtg acggcaccga
     4021 gcgagaaccg ctcggagtac acgtcgcgca tcagcaccgg cagcagcagg ccgcccagtg
     4081 cgaggatcgt gacccccgag gcgcccgtga acgtggtgaa gaaggcgcag accaggatgg
     4141 tcgcgaccgc cgtgccaccc ggcagccacc cggccagcgc cttgaacagg cgcaccagcc
     4201 gggcgggcgt gcccccctcg gccagcagca cgccggccag cgtgaagagc gggatggttg
     4261 gcagcgtggg cgacgacacg atgccgtagg tctggctcgg gaccgccgca agcggcacga
     4321 ggtcggcgta gaagagaacg agcgccgcgc cccccagcgt gacgaagatc ggcgctccca
     4381 tcaccgtggc cgccagcagg aggacgatca ggggccatcg caccgagggg gccaccgcct
     4441 cgggggcgaa ggcgaggctg acggttgccg cgacgacggc cgccacgatc aggcgccgtt
     4501 cccacgcggc ccgggccggc aggggaggcg ccggctggtc gcccacgccg acgggcacac
     4561 cggcctgcca ccacgcgcgc agggcgacca ccgcgaaccc gaacgggatc acggtctgca
     4621 cc
//
LOCUS       DAOPQX010000304         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20358_length_4602_cov_4.775896, whole genome shotgun sequence.
ACCESSION   DAOPQX010000304 DAOPQX010000000
VERSION     DAOPQX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20358_length_4602_cov_4.775896"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKK95_13805"
     CDS             complement(<1..822)
                     /locus_tag="PKK95_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOC19343.1"
                     /translation="MNRLGLALVVLQCLAGLGAAQEAADFRPASTNVWDAQYPRVDGK
                     GRVEIRLKAPEATTVRVNFWSGPKVAMVKQPDGVWSVITEPLVPGLHYYTLIVNGVEM
                     ADPGSRAFFGGSKYASAVEVPEPGSTYYLPQDVPHGQVREVWYFSKVTGTWRHALVYT
                     PPGYDEKTAERYPVLYLQHGGGEDETGWVRQGHANFILDNLIAAGKAKPMLVVMAYGY
                     ARRAGQPAPDLAGRAFGSPEFRKSIQDMMAAFEAEVTEVLIPFIDKTFRTIADRDH"
     gene            1002..2078
                     /locus_tag="PKK95_13810"
     CDS             1002..2078
                     /locus_tag="PKK95_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOC19344.1"
                     /translation="MTTRNLPAALVLAVTLAAGLQLAAQVKTEVPAPVPGAKPVSVER
                     VKIHGPSLEGNLEGNAVDRDALVFLPPSYAREKSRRYPVVYALHGYSIGAEQWAGEIH
                     VPQTIEGAFALGVSEVIVVLPDSKTVHNGSMYSSSVTTGDFERYVAKDVVSYMDAHYR
                     TIARRESRGLVGHSMGGYGATRIGMKHADVFGSLYIMSPCCLSARGAGREPSEVEKAL
                     EKVKTPADSASLPFFPRAQLATSAAWSPNPKNPPLYLDLPTKGGELQPDVLARWAANA
                     PLAFVDQYISDLRKYRAIAMDVGDQDGLRMDAARLHEVFDRYGLAHTFEIYPGTHTSR
                     VADRFQNHVLPFFGKSLAFEKPRQ"
     gene            complement(2097..3173)
                     /locus_tag="PKK95_13815"
     CDS             complement(2097..3173)
                     /locus_tag="PKK95_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19345.1"
                     /translation="MRRHSGCPIACLTLLVLAAAAATAFAQAPQTYTITMKQGPNNTT
                     IVTSRDGSKESVEQKVPPSKIGPGMHVRLLYDLAAHKLWSMDLEGGPCSVVAYTSPEV
                     PGPFDPISSGSETMARMSKEKLTPLRSETINGLATKLYEIPAPEIKGKVLLFVEEKQN
                     FPVKQVFAFGDGREITAMEITSLSFDKPPADLFVPPKGCQEIAGSTSATGGHAETQVE
                     EKAAGEQQLGEKQAAPRRAPARASGSARTAAAAAVTEVRAVGVQPSHYTAAAPAAYVF
                     TFAITASGPLEAQWVLVSQADTAWASGKLAFEAAGTKELEVPVKIGVGNGQHWEGSGH
                     LEVVVSGKRTFSEKLPVSADCQAK"
     gene            complement(3270..3346)
                     /locus_tag="PKK95_13820"
     tRNA            complement(3270..3346)
                     /locus_tag="PKK95_13820"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3310..3312),aa:Arg,seq:tct)
     gene            3497..3964
                     /locus_tag="PKK95_13825"
     CDS             3497..3964
                     /locus_tag="PKK95_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC19346.1"
                     /translation="MGDQDLDELAEGEGESPMRSQPLVCVRDVEASSRWYQHLLGCRS
                     DHGGPEYERLKANGRLVLQLHRWDVGHHHGPIGNPDLAHGNGVLLWFETEDFDQAVAR
                     AGELKAEVVMARHRNPPDGDGGPNHWELWLRDPDGYTVVLSSPDGTADGNWKP"
     gene            4063..>4602
                     /locus_tag="PKK95_13830"
     CDS             4063..>4602
                     /locus_tag="PKK95_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19347.1"
                     /translation="MLARLSVVALLLALAPVPATGQAHKPPSGMDTYLFGIVRTGHVA
                     PAPAAEAKTLQEGHLRNMERMADEGLLVGAGPLRDAGDYRGIFIFKADRREDVEKRVQ
                     DDPLIRSGRLVLELLPWWGPEKIGERYFAAKRHDPVAPDRMVAYQLAFLKAGPNRKPD
                     VDPETEKIQAGHMAHIQKMA"
BASE COUNT          742 a         1583 c         1555 g          722 t
ORIGIN      
        1 gtgatcgcgg tccgcgatcg tgcggaacgt cttgtcgatg aacgggatca gcacctcggt
       61 gacctcggcc tcgaaggccg ccatcatgtc ctggatcgac ttccggaact cgggcgagcc
      121 gaaggcccgg ccggcgagat cgggcgcggg ctggccggcg cgccgcgcgt agccgtacgc
      181 catcaccacg agcatcggct tcgccttgcc ggccgcgatc aggttgtcga ggatgaagtt
      241 cgcgtgcccc tggcgcaccc agcccgtctc gtcctcgccg ccgccgtgct gcaggtacag
      301 cacggggtac cgctcggccg tcttctcgtc gtaacccggc ggcgtgtaca cgagggcgtg
      361 gcgccaggtg ccggtcacct tcgagaagta ccacacctcg cgcacctgcc cgtggggcac
      421 gtcctgcggc aggtagtagg tcgagccggg ctcgggcact tccaccgcgc tcgcgtactt
      481 gctgccgccg aagaacgcgc ggctgcccgg gtcggccatc tccacgccgt tgacgatgag
      541 ggtgtagtag tgcagccccg gcacgagcgg ttcggtgatc acgctccaca cgccgtcggg
      601 ctgcttcacc atggcgacct tcggcccgct ccagaagttc acgcgcaccg tggtggcctc
      661 gggggccttc agcctgatct cgacccgccc cttgccgtcc acccgcgggt actgcgcatc
      721 ccagacgttg gtggaggcgg ggcggaagtc ggcggcttcc tgcgccgcgc ccagccccgc
      781 gagacactga aggacgacca gcgccagtcc aagtcggttc atcgggttgc tcctcgtcgg
      841 tgagccgccc acgcggccca gcaggtcgtg agtgtaaacg cttaaccgcg acggcggaag
      901 cggagaaatc ggtcagcgct cgcaggcgga gcggctgttg acagagggtg ttgaaagcgc
      961 gtacgttctc gggcgcccca ccccaacgtg aggagacccg gatgacgacc aggaacctgc
     1021 ccgccgccct cgtgctcgcc gtgacgctcg ccgcgggttt gcagctcgcc gcgcaggtga
     1081 agacggaagt gccggcgccg gtgcctggcg ccaagccggt gagcgtcgag cgcgtcaaga
     1141 ttcacggccc gtcgctcgag gggaacctcg aggggaatgc cgtcgatcgg gacgccctcg
     1201 tgttcctgcc gccgagctac gccagggaga agtcgcgccg ctacccggtc gtctacgcgc
     1261 tgcacgggta ctcgatcggg gcagagcagt gggccggaga aatccacgtg ccgcagacga
     1321 tcgagggcgc gttcgcgttg ggcgtcagcg aggtgatcgt cgtcctgccc gactcgaaga
     1381 cggtgcacaa cggctcgatg tactcaagct cagtcaccac cggtgacttc gagcgctacg
     1441 tcgcgaagga cgtcgtctcg tacatggatg cgcactaccg gacgatcgcg cggagggaga
     1501 gccgcggcct cgtcggccac tcgatgggcg ggtatggcgc gacccgcatc ggcatgaagc
     1561 acgccgacgt cttcggcagc ctctacatca tgagcccgtg ctgcctgtcg gcgcgcggcg
     1621 cgggacgcga gccctcggag gtggagaagg cgctcgaaaa ggtgaagacc cccgccgact
     1681 cggcgtcgtt gcccttcttc cctcgcgcgc agctggcgac ctcggcggcc tggtcgccca
     1741 acccgaagaa cccgccgctc tacctcgacc tgccgaccaa gggcggcgag ctgcagcccg
     1801 acgtcctcgc ccgctgggcc gccaacgcgc cgctggcctt cgtggaccag tacatcagcg
     1861 acctccggaa gtaccgcgcg atcgcgatgg acgtcggcga ccaggacggg cttcggatgg
     1921 acgcggccag gctgcacgag gtgttcgacc ggtacggcct cgcccacacc ttcgagatct
     1981 acccgggcac gcacaccagc cgggtggccg accgcttcca gaaccacgtg ctgccgttct
     2041 tcggcaagag cctggcgttc gagaagccgc gccagtaggc gagggggcgg cgtcttctac
     2101 ttcgcctggc agtccgccga cacgggaagc ttctcggaga aggttctctt gccgctcacg
     2161 accacctcga ggtgcccgga cccttcccag tgctggccgt tgccgacgcc gatcttcact
     2221 ggaacctcga gttccttcgt gccagctgcc tcgaaggcca gcttgcctga tgcccacgcg
     2281 gtgtcggcct ggctcaccag cacccactgg gcctcgaggg gaccggaggc agtgatcgca
     2341 aaggtgaaca cgtaggcagc gggtgcggcc gccgtgtagt gcgagggttg aactccgacg
     2401 gcgcgcacct cggtgaccgc ggcggcggct gcggttcgcg cgctgccgga cgcccgcgcg
     2461 ggagcccgcc tcggcgcagc ctgcttctca cccagctgct gctcgccggc agccttctct
     2521 tcgacctgcg tctcggcgtg gccgcccgtg gcgctcgtcg agccggcgat ctcctggcag
     2581 ccctttggcg gcacgaagag gtcggccggc ggcttgtcga acgacaggct cgtgatctcc
     2641 atcgccgtga tttccctgcc gtctccgaat gcaaagacct gcttcacggg gaagttctgc
     2701 ttctcctcga cgaagagcag caccttgccc ttgatctccg gcgccgggat ctcgtagagc
     2761 ttcgtcgcga gaccgttgat ggtctccgac cggagcggcg tcagcttctc cttcgacatc
     2821 cgggccattg tctcggaccc ggaactgatc gggtcgaagg ggccggggac ctctggagac
     2881 gtataggcga cgaccgagca cggtcctccc tcgagatcca tcgaccacag cttgtgtgcg
     2941 gcgagatcgt acagaagccg gacgtgcatt ccggggccga tcttgcttgg cggcactttc
     3001 tgctccaccg actccttcga accatcccgg gacgtcacga tggtggtgtt gttcggtccc
     3061 tgcttcatcg tgatggtgta ggtctgggga gcctgggcga acgccgtcgc ggcggccgct
     3121 gcaagcacca ggagcgtcag gcaggcgatc gggcagccgg agtgtcttcg cattggagtc
     3181 ctctaggggc cgcacacgtg acgacgagaa ggctagcggc agagtttaca ccactgtgac
     3241 ccatgtctcc ggaatagacc taggaaggct ggcgcgcccg gctggactcg aaccagcgac
     3301 cctcagctta gaaggctgat gctctatcca cctgagctac gggcgcacgt gtgggaagcg
     3361 ggggatggcg cgacgcgggc gccgccccgc gtgtcatgat agccctatca gggcacgccc
     3421 gcgaacggcg gcctgcagga acacgcgccg gcggaaggag aaggtgaatc ccccatgcgc
     3481 cctcaacgag ccgccggtgg gcgaccagga tctcgatgaa cttgcggaag gagaaggtga
     3541 atcccccatg cgctctcaac ccctcgtctg tgtgcgcgac gtcgaggcga gcagccgctg
     3601 gtaccagcac ctgctcggct gccgcagcga ccacggcggc ccggagtacg aacgcctcaa
     3661 ggccaatggc cggctcgtcc tgcaactgca ccgatgggac gtggggcacc atcacggccc
     3721 gatcggcaat ccggacctcg cccacggcaa tggtgtgctc ctctggttcg agaccgagga
     3781 cttcgaccag gcagttgccc gcgcgggcga actgaaggcg gaggtcgtca tggcgcgcca
     3841 ccggaacccg cccgacggcg acggcggccc gaaccactgg gagctctggc tccgcgaccc
     3901 cgatggttac acggtcgtcc tgtccagccc cgacggaacc gcggacggca attggaagcc
     3961 gtgagccgcg ggcgaccacg ggatcgggcg gccacaaggg tcgcccggtg tcgatgacat
     4021 ggcttgggcg gccacaaggg tcgcccctac gatggagggg ttgtgctcgc tcgcctcagc
     4081 gtagtcgcgt tgctgctcgc gctcgctcct gtgccggcca ccgggcaggc ccacaagccg
     4141 ccctccggca tggacaccta cctgttcggt atcgtcagga ccggccacgt cgcgccggcg
     4201 cccgccgccg aggcaaagac cctccaggag gggcacctcc gcaacatgga gcgcatggcc
     4261 gacgaagggc tgctcgtcgg cgcggggcct ctccgcgacg cgggcgacta ccgcggcatc
     4321 ttcatcttca aggccgatcg ccgcgaggac gtggagaaga gagtccagga cgacccgctg
     4381 atccgttccg ggcgcctggt gctcgaactt ctgccgtggt ggggaccgga gaagatcggc
     4441 gagcgctact tcgcggcgaa acgccacgac ccggttgcac ccgaccggat ggtcgcctac
     4501 cagctcgcct tcctgaaggc cggtccgaac cgcaagcccg acgtcgatcc cgaaaccgag
     4561 aagatccagg ccggccacat ggcgcacatc cagaagatgg cg
//
LOCUS       DAOPQX010000305         4596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20398_length_4596_cov_3.695662, whole genome shotgun sequence.
ACCESSION   DAOPQX010000305 DAOPQX010000000
VERSION     DAOPQX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20398_length_4596_cov_3.695662"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PKK95_13835"
     CDS             complement(<1..975)
                     /locus_tag="PKK95_13835"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOC19348.1"
                     /translation="MQGNPTDFWGKLRRAEDGRTVAEWHPLYDHCADVAAVTEALLAL
                     PLWRRRLSRLAGQDLDEVLCARLSVLAALHDIGKFNLGFQAKGLPELGPTAGHVAEGL
                     AALAYGGVLGCLASLEEWGDGTLDLLVASMCHHGRPIRVEAAGSSWQRTWWSPRRGFD
                     PIAGVVGLLDRCRRSWFHHAFRPGARLALTPNLTHAFAGLVMLTDWLASDTRLFPFTR
                     TPGADRMEYARSVAPASLRALGLSVDLADRCDPFRRDAFTRVIPEPFSPRGAQRAMLE
                     LPLNDQGSIAVLEAETGSGKTEAALAYFVRLFEAGLVDGMYFALPTHSA"
     gene            complement(1319..2860)
                     /locus_tag="PKK95_13840"
     CDS             complement(1319..2860)
                     /locus_tag="PKK95_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOC19349.1"
                     /translation="MSNADRLSHLEKLAEAGGGEARLQRQHQAGKLAARERMALLFDP
                     GTFEEYDKLVTHRCRDFGMDEQQVPGDGVVSGYGRVEGRPAYAFAQDFTVFGGSLSET
                     NAAKIVKIMDLAMRQGCPVVGLNDSGGARIQEGVASLAGYADIFLRNTLASGVIPQIS
                     AIMGPCAGGAVYSPAITDFTVMVEKTSYMFVTGPDVIRTVTHEDVSKEDLGGAMTHNA
                     RSGVAHFAVADDRECLLLIRDLLGFLPSNNLDEAPRRESGDPPDREDESLDRLVPDSP
                     NQPYDILDLIHAVVDDGNFLEVHAHYARNLVVGFARLGGRSVGIVANQPAHLAGTLDI
                     DASVKGARFVRFCDAFNVPLVTFEDVPGFLPGTMQEFGGIIRHGAKLLYAFAEATVPK
                     VTVIARKAYGGAYCVMASKHIRTDLNLAWPTAEIAVMGPEGAVNILYKRELDGAADRE
                     ALRAAKVGEFREKFANPFVAAARGFIDEVIRPRTTRRRLIAALAALETKRDKNPPKKH
                     GNIPL"
     gene            complement(2906..3679)
                     /locus_tag="PKK95_13845"
     CDS             complement(2906..3679)
                     /locus_tag="PKK95_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOC19350.1"
                     /translation="MKIFRVDLDGREQYLLALEGRFHRLEGDPFGEFTPGDDLGPGES
                     AASLPAGARLLAPVVPTKIVGIGLNYRDHAAEQKKPLPPEPLVFIKPASAVIAGGEAI
                     CLPPGVGRVDHESELAVVIRRRATKVARERAFDYVLGYTCANDVTARDLQNRGVQYSH
                     CKGYDTFAPLGPCIATRLDPAGLAVQGRLNGELRQDSSTRQLIFPVDRLIEYISAIMT
                     LEPGDVISTGTPSGIGRLAPGDVFTVTVEGVGDLTNVVK"
     gene            complement(3695..>4596)
                     /locus_tag="PKK95_13850"
     CDS             complement(3695..>4596)
                     /locus_tag="PKK95_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19351.1"
                     /translation="RKKKEEPEPELPESAYSPSAGGWVEMSPPLGPPRLAKDVQRVYM
                     AVALTRRGRRGAFTEPMAVSLQPPPSQPASVAARYDEKTISVSWEAPPDLRRPTLPPR
                     SNAELLDAKPLGMPSLAGGFNTFVAAPASEAANGSAGQEGGGVVTLGPAPAPLNSAPL
                     SEPRFEEPVPAGAAERCYTVTAISTYSAGPVQSEPSPPVCVALKDVFPPAPPKNLAAV
                     GSEGAVSLIWERSEASDLAGYLVLRSEGRGKPGALNETPIAETTFRDERVKPGVVYRY
                     VVVAVDKAGNRSAPSNAAEESAR"
BASE COUNT          697 a         1621 c         1586 g          692 t
ORIGIN      
        1 ggcgctgtgg gtgggaagcg cgaagtacat cccatccacg agtcccgcct cgaacaggcg
       61 aacgaagtac gcgagcgcgg cctcggtctt cccgctcccc gtttcagcct cgagtaccgc
      121 gatcgaaccc tggtcgttca gcgggagttc gagcatggcc cgctgtgccc cacgaggaga
      181 gaagggttcg gggatcacgc gagtgaaggc atcacgacga aatggatcgc accggtcggc
      241 caggtcgact gagagcccaa gagcccgtag cgacgcgggg gcgaccgacc gcgcgtattc
      301 catcctgtcc gcaccgggcg tgcgggtaaa cggaaacagc cgcgtgtccg aggccagcca
      361 gtcggtgagc atgaccaggc ccgcgaacgc gtgcgttaag ttcggggtga gcgcaagcct
      421 cgcgcccggt cggaaggcgt gatgaaacca gcttcggcgg cagcggtcga ggaggccgac
      481 aactccggcg atcggatcga acccccgccg tgggctccac caggttcgct gccacgatga
      541 gcccgcggct tcaaccctga tcgggcgtcc gtggtggcac atcgatgcga cgagcaggtc
      601 gagggtccca tcaccccact cttcgaggct ggcaaggcag ccaagcacgc cgccgtaggc
      661 gagggcggcc agcccttcgg ccacgtgacc cgccgtgggg cccagttcgg gaaggccctt
      721 ggcctggaac ccgagattga acttgcctat gtcgtgcaat gcggcaagga cggaaagccg
      781 cgcgcagagg acctcgtcga gatcctgccc ggccaggcgc gaaagacgcc gtcgccagag
      841 cggaagcgcc agcagtgcct cagtgactgc tgccacgtca gcacagtggt cgtacagcgg
      901 gtgccattcg gccacagtgc gcccatcttc cgcgcggcgc agttttcccc agaagtcggt
      961 cgggttgcct tgcacctatg tccccagtcg acgcagccgg cgggcgcatt ctgacacagc
     1021 gtgaggagca agtcagaatg aagtcacaat tcggacccac gcgctccgca cgcgcgccca
     1081 tgctgcggta ggtttgtcac accagcgtga caggacaggt cacagatata ggcagtggaa
     1141 gggacgctcc cggggttgtg gctgggacga ctccgccgcg tctcgcgtag cggcgaaagc
     1201 gggacttcag gacttcgtgg actcgacctc gcgacttcgg gacttcgcgg cctgccgcgc
     1261 cgtagccgct cagcggcgaa ggcgggactt cgtgacttcg tggactgtac cgctacagct
     1321 acagcggtat gttcccgtgc ttcttcggcg ggttcttgtc gcgcttggtc tcgagcgccg
     1381 ccagcgccgc gatcagccgg cgccgcgtcg tgcgcgggcg gatcacctcg tcgatgaacc
     1441 cgcgggccgc cgccacgaac gggttcgcga acttctcccg gaactccccc accttcgccg
     1501 cccggagcgc ctcgcgatcc gcggccccgt ccagctcgcg cttgtagagg atgttcaccg
     1561 ccccctcggg ccccatcacc gcgatctccg ccgtcggcca cgcgagattg agatcggtgc
     1621 ggatgtgctt gctcgccatc acgcagtacg cgccgccgta ggccttccgc gcgatcacgg
     1681 tcaccttcgg gacggtcgcc tcggcaaacg cgtacagcag cttcgccccg tgacggatga
     1741 tcccgccgaa ctcctgcatc gtcccgggga ggaacccggg cacgtcctcg aaggtcacca
     1801 gcggaacgtt gaacgcgtcg cagaagcgca cgaagcgcgc ccccttcacc gaggcgtcga
     1861 tgtcgagcgt ccccgccagg tgcgccggct ggttggcgac gatccccacg ctgcggccgc
     1921 ccagccgggc gaagcccacc acgaggttcc gcgcgtagtg cgcgtgcacc tcgaggaagt
     1981 tcccatcgtc cacgaccgcg tggatcaggt cgaggatgtc gtagggctgg ttcggcgagt
     2041 cgggaacgag gcggtcgagc gactcgtcct cgcggtcggg ggggtccccc gactcgcggc
     2101 gcggagcctc gtccaggttg ttcgagggga ggaagccgag caggtcgcgg atcaggagga
     2161 ggcactcgcg gtcgtcggcc accgcgaagt gcgccacccc gctgcgcgcg ttgtgcgtca
     2221 tcgcgccgcc gagatcctcc ttgctgacgt cctcgtgcgt caccgtgcgg atcacgtcgg
     2281 ggccggtgac gaacatgtag ctggtcttct cgaccatcac cgtgaagtcg gtgatggccg
     2341 gcgagtagac ggcgcccccc gcgcaggggc ccatgatggc ggagatttgc gggatgacgc
     2401 ccgaggcgag cgtgttgcgc aggaagatgt cggcgtaccc ggcgagcgac gccacccctt
     2461 cctggatccg cgcgccgccc gagtcgttca gcccgaccac cgggcacccc tggcgcatcg
     2521 cgaggtccat gatcttcacg atcttggcgg cgttggtctc ggagagcgag ccgccgaaga
     2581 cggtgaagtc ctgcgcgaag gcgtacgccg ggcgcccctc gacacgcccg taccccgaca
     2641 ccacgccgtc gccgggaacc tgctgctcgt ccatgccgaa gtcgcggcac cggtgggtga
     2701 ccagcttgtc gtactcctcg aaggtcccgg ggtcgaagag gagcgccatg cgctcgcggg
     2761 cggcgagctt gccggcctgg tgctggcgct gcagccgcgc ctcgccgccg ccggcctcgg
     2821 ccagcttctc gaggtgggag agcctgtcgg cgttcgacat gggggccatc cttgtcgtca
     2881 cggcgggcgg ccacaagggc cgcccctact tcaccacgtt ggtgagatcg cccacgccct
     2941 cgacggtgac ggtgaacacg tcgccgggcg ccaagcggcc gatgcccgac ggcgtgccgg
     3001 tcgagatcac gtcgccgggc tccaaggtca tgatcgcgga gatgtactcg atgaggcgat
     3061 ccaccgggaa gatcagctgc cgcgtggacg agtcctggcg cagctcgccg ttcagccgcc
     3121 cctgcacggc gaggccggcc gggtcgagcc gcgtcgcgat gcacgggccg agcggcgcga
     3181 aggtgtcgta ccccttgcag tgcgagtact gcacgccgcg gttctgcagg tcgcgggccg
     3241 tgacgtcgtt cgcgcaggtg tagccgagga cgtagtcgaa cgcgcgctcg cgcgccacct
     3301 tcgtcgcacg cctgcggatc accaccgcca gctcgctctc gtggtccacg cgtcccacgc
     3361 cggggggaag gcagatggcc tcgccccccg cgatcacggc cgaggccggc ttgatgaaca
     3421 ccaacggctc gggcggcagc ggcttcttct gctcggccgc gtggtcgcgg tagttcagcc
     3481 cgatcccgac gatcttggtg gggacgaccg gcgccagcag ccgcgccccg gcaggcagcg
     3541 acgccgcgct ctcgccaggc ccgaggtcgt cgcccggcgt gaactcgccg aagggatcgc
     3601 cctcgagccg gtggaagcgg ccctcgaggg cgaggaggta ctgctcgcgg ccatcgagat
     3661 cgacgcggaa gatcttcata gcgtctccga ccagctaccg tgccgattcc tcggcggcgt
     3721 tcgagggcgc gctgcggttg cccgccttgt ccaccgccac caccacgtac cggtagacga
     3781 cgcccggctt cacgcgctcg tcgcggaagg tcgtctcggc aatcggcgtc tcgttcaacg
     3841 ccccgggctt tccacggccc tcggaccgca gcaccaggta gccggcgagg tcggacgcct
     3901 cgctgcgctc ccagatcagg ctcaccgcgc cctcgctgcc cacggccgcc aggttcttcg
     3961 gcggcgccgg cgggaagacg tccttcagtg cgacacacac gggcggcgac ggctcgctct
     4021 gcaccgggcc ggccgagtac gtgctgattg ccgtcacggt gtagcagcgt tcggccgccc
     4081 cggcgggcac cggctcctcg aagcggggct cggagagcgg cgcgctgttg agcggcgcgg
     4141 gtgccgggcc gagggtcacc acgccgccac cctcctgccc cgcgctcccg ttggcggctt
     4201 cggaagcagg cgcggccacg aacgtgttga atcccccggc cagcgagggc atgccgagcg
     4261 gcttcgcgtc gagcaattcg gcgttcgacc gcggtggcag cgtcggccgc cgcagatcgg
     4321 gcggcgcctc ccacgacacc gagatcgtct tctcgtcgta acgcgcggcc accgacgcgg
     4381 gctgcgacgg cggcggctgc agcgacaccg ccatgggctc ggtgaacgca cccctccggc
     4441 cccggcgtgt gagcgcaacc gccatgtaca cccgctggac gtccttggcg agccgaggcg
     4501 gcccgagcgg cggcgacatc tccacccacc cgccggcact ggggctgtac gccgactcag
     4561 ggagctcggg ctcgggttct tccttcttct ttcgcg
//
LOCUS       DAOPQX010000306         4590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20442_length_4590_cov_4.444542, whole genome shotgun sequence.
ACCESSION   DAOPQX010000306 DAOPQX010000000
VERSION     DAOPQX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20442_length_4590_cov_4.444542"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..735)
                     /locus_tag="PKK95_13855"
     CDS             complement(232..735)
                     /locus_tag="PKK95_13855"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOC19352.1"
                     /translation="MLGASPPALAREGAPWSGLLPFSSPALALPAATIVAADAPPVVA
                     PDLAEPIAPLPPLVVPASAGPAAGRPSALVALYVSYAGLQALDAVTTLRALDAGASEQ
                     NPLVGRAAAHPGALLAVKAGVAVGTIYLAERLRKRNRAAAVVLMAVLNGAYLAIVANN
                     HSLGGKR"
     gene            937..2340
                     /locus_tag="PKK95_13860"
     CDS             937..2340
                     /locus_tag="PKK95_13860"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOC19353.1"
                     /translation="MPSYFAVLILAVVAWGAFTFGAVYQWAAVPLFWASGAIGLLGLI
                     APGTSRPGRLNAALAAGLALVAAAAAAQLVPLDAATLARLTPNTHAFLTQYVVGYPDV
                     VTAHPLSIRPVATRYGLAAFASFSLLLLGAARGLGRRSARTLAGGLLAVGFALALTGI
                     VQSGLSGRGLASSRIYGLVTAAHASPFGPFLNRNHFAGWMLLALPVGLGLFIGQVARG
                     MRGAGPTLRERVLWFSSPQASRVVLAGLAVLVMGVSLVLTFSRSGIGGFATALALSAF
                     FVIRRQSRGSRRAVALSYVAVLLALSLGWAGLDAIVARFADLQGTGLNGRLGVWGDAW
                     RVVRAFPWTGTGLNTFSEAMEVYQTFEMTTHTMEAHNDYLQLLADGGLLLALPALIAI
                     ILLGREIRRRFREARDDTTSYWLRVGATTALVAIAVQETVEFSLRLPGHGVLLAAVVA
                     VALHRSDGSRPGDRGIG"
     gene            2368..2661
                     /locus_tag="PKK95_13865"
     CDS             2368..2661
                     /locus_tag="PKK95_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19354.1"
                     /translation="MRISWLKEIVVQSCPKCRSTRIHRSRSRGWFERFRKGFTNKRPH
                     RCHACGWRGWGLESDNHREKGRSAGIDCGLPDLTAIDEAIGFERDGASKRASR"
     gene            2744..3091
                     /locus_tag="PKK95_13870"
     CDS             2744..3091
                     /locus_tag="PKK95_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC19355.1"
                     /translation="MPIYEFECKDCHRKTTALVMSRERIGEVRCRRCGSANLEKLFSR
                     FATPKSEDARLESLADPSAMAGVDENDPQSVARFMKRMGKEMGEDFGDDIDQAIEEEM
                     AGGAEGSEGGDES"
     gene            3118..3864
                     /locus_tag="PKK95_13875"
     CDS             3118..3864
                     /locus_tag="PKK95_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19356.1"
                     /translation="MSLRIAFDLDGTVADLDSALAEISRSLFGGEPEAVAPAEGPPAE
                     VRRGDPRGRLPADTPAAKDGPDEEEDRVTPPRVRSLTPRQQSMVWQAALRTPNFWETL
                     GEIEPGVLARLWALAGERGWEVVFLTQRPASEGDTTQRQSQRWLRAKGFELPSVFVVS
                     GSRGKVASALGLDVVVDDRPENCLDVKVDSTARAVLVARAGSPAMAANAARLGIEAVS
                     SIAELLDRLGRRAADGPSLLGRIKNLLTGA"
     gene            complement(3930..>4590)
                     /locus_tag="PKK95_13880"
     CDS             complement(3930..>4590)
                     /locus_tag="PKK95_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19357.1"
                     /translation="RERPAKPERPREGRPPQERPSKPERPREGRLPHERPWKPERPRE
                     GRPTHQRPWKPERPREGRPPQQPPPGGEKRGGDRQGHDRPRGEMKPRGAKTEWRPKEP
                     WRPKGEWKPKGEQPKGEWRPKGEWKPRGTGAAKPRPDRFAERNAGRGQDQPPPPRTDE
                     PPVTPEPERPRGPDRPPAPSENPAPPKPAPERIEVVPDPPERARTAPPHKTKLPRLRK
                     P"
BASE COUNT          551 a         1619 c         1740 g          680 t
ORIGIN      
        1 ccagcgttgg gagtcggagc ctcctgcccg cttcccgaac tacgcggccg gcaccgccgg
       61 ccccgccgag gccaccgagc tcctggcgcg tgaagggcgg cggtggaggg agctgtagcc
      121 cgcccgcaac cgcgatcggt gctgggcgga cggcgggtgc gtcagcgaac gagcccgtgc
      181 ggagacagga gacaggagac aactgacgcc gcacttggag ccgcgggccc cctaccgctt
      241 cccgcccagc gagtggttgt tcgcgacgat ggcgaggtag gcgccgttga ggacggccat
      301 cagcacgacc gccgccgccc ggttcctctt ccgcaaccgc tccgccaggt agatcgtgcc
      361 gaccgcaacc cccgccttca ccgccagcag cgcgcccggg tgcgcggccg cgcggccgac
      421 caacgggttc tgctcgctcg ccccggcgtc cagcgcgcgc agcgtcgtca cggcatcgag
      481 cgcctgcagc cccgcgtacg agacgtaaag cgcaaccagg gccgaggggc gcccggccgc
      541 gggcccggcg ctcgcgggga cgacaagcgg gggaagcggc gcgatcggct cggcgagatc
      601 cggcgcgaca accggcgggg cgtcggccgc gacgatcgtc gcggccggca gcgccagcgc
      661 cggcgacgag aacggcagca gcccgctcca tggcgcgcct tcccgcgcga gggctggcgg
      721 cgaggcgccg agcatcgcga cgatcggcag caggacaagg cgcgggagta cacgacgggc
      781 gcgatggggg cagggggcgg ctggtcgcat gggggctggc cagcggccct gccagaacga
      841 tgccaggaag cggggagagc cacgcgccgc ggcgggcggt gctcgggacg gcgcgggccg
      901 ttgccccgcc gcatcgggta tcatgtgccg ctgctcgtgc cttcgtactt cgccgtcctg
      961 atcctcgccg tcgtcgcctg gggcgccttc acgttcggcg cggtctacca gtgggccgcg
     1021 gtgcccctgt tctgggcctc gggcgccatc ggcctcctcg ggctgatcgc cccgggaacc
     1081 tctcggccgg gccggctgaa cgccgcgctc gccgccgggc tggcgctggt tgccgcggcc
     1141 gccgccgctc agctggtccc gctcgacgcg gcgacgctcg cgcgcctcac tcccaacacc
     1201 cacgccttcc tcacccagta cgtcgtcggc taccccgacg tggtcaccgc ccaccccctg
     1261 tcaattcgcc ctgtagcaac ccgctacggg ctcgcggcct tcgccagctt ctccctcctg
     1321 ctgctcggcg cggcgcgagg cctagggcgg cgaagcgcgc gcacgctcgc cggaggcctc
     1381 ctcgccgtgg gcttcgccct cgccctgacc ggcatcgtcc agtcgggctt gagcggacgc
     1441 gggctggcgt cgagccggat ctacgggctg gtcaccgcgg cccacgcctc gccgttcggc
     1501 ccgttcctca accggaacca cttcgccggc tggatgctgc tggcgctgcc ggtcggcctc
     1561 ggcctgttca tcggccaggt ggcgcgcggg atgcgcgggg cagggccgac gctgcgcgag
     1621 cgggtgctgt ggttctcgtc gccgcaggcc agccgcgtcg tgctcgcggg gctcgcggtc
     1681 cttgtgatgg gggtgtcgct cgtcctcacc ttctcgcggt cggggattgg cggcttcgcg
     1741 accgcgctgg cgctctcggc cttcttcgtc atccggcgcc agtcgcgcgg ctcgcgccgc
     1801 gccgtcgcgc tgtcgtacgt cgcggtgctg ctggcgttga gcctcgggtg ggccggcctc
     1861 gacgcgatcg tcgcgcggtt cgccgacctg cagggcaccg gcctcaacgg gaggctgggc
     1921 gtgtggggcg acgcgtggcg ggtcgtgcgg gcgttccctt ggacggggac cggcctgaac
     1981 accttcagcg aggcgatgga ggtgtaccag accttcgaga tgacgacgca cacgatggag
     2041 gcgcataatg attacctcca gctgctggcc gacggcggcc tgctgctggc cctgcccgcc
     2101 ctcatcgcga taatcctgct cggccgcgag atccgccgcc gcttccgcga ggcgcgagac
     2161 gacacgacct cgtactggct gagggtggga gcgacgacgg cgctggtggc gatcgcggtg
     2221 caggagacgg tggagttcag cctgcggctc cccggccacg gcgtgctgct ggccgccgtc
     2281 gtcgcggtgg cgctgcaccg gagcgacggc agcaggcctg gcgatcgagg aatcgggtga
     2341 agccccaggg ccgattccat tctcactttg cggatatcgt ggctgaagga gatcgtggtg
     2401 cagtcttgtc cgaagtgccg gtcgacgcgg atccaccgtt cgcgctcccg cgggtggttc
     2461 gagaggtttc gaaaggggtt caccaacaag cggccgcacc ggtgccacgc ctgcgggtgg
     2521 cgcggctggg gcctcgagag cgacaatcac cgggagaagg gacgttcggc ggggatcgat
     2581 tgcggcctgc ccgacctcac ggcgatcgac gaggccatcg gcttcgagcg ggacggcgct
     2641 tcgaagagag cgagcaggta agaccggaca ggcggggtga agtgcgtgga caagtcgccg
     2701 cgtctgttgc gtacgaaggg ctggtgtaaa tcgggattga gagatgccga tctacgagtt
     2761 cgagtgcaag gactgccacc ggaagacgac cgcgctcgtg atgtcgcgcg agcggatcgg
     2821 cgaggtgcgc tgccggcgct gcgggagcgc gaacctcgaa aagctgttct cgcgcttcgc
     2881 cacgccgaag tcggaggacg cgcgcctcga gtcgctggcc gacccgtcgg cgatggccgg
     2941 cgtggacgag aacgacccgc agagcgtcgc ccgtttcatg aagcggatgg gcaaggagat
     3001 gggcgaggac ttcggcgacg acatcgacca ggcgatcgag gaggagatgg cgggcggcgc
     3061 cgaggggagc gagggcggtg acgaatcctg actcctgaat cccgaatcct gactcctgtg
     3121 tcccttcgca ttgccttcga cctcgacggc accgtggccg acctcgactc cgcgctcgcg
     3181 gagatcagcc ggtccttgtt cggcggcgag cccgaggcgg tggcgccggc ggaggggccg
     3241 cccgccgagg ttcgtagggg cgaccctcgt ggtcgccttc cggcggacac gccggccgcc
     3301 aaggacggcc cggacgagga agaggaccgg gtcacgccgc cgcgcgtgcg gtcgctgacg
     3361 ccgcgccagc agtcgatggt gtggcaggcc gcgctccgca cgccgaactt ctgggagacg
     3421 ctgggggaga tcgagccggg cgtgctggcg cggctgtggg cgcttgccgg cgagcgcggc
     3481 tgggaggtgg tcttcctgac ccagcggccc gcgtccgagg gcgacaccac gcagcgtcag
     3541 agccagcggt ggctgcgcgc gaagggcttc gagctgccca gcgtcttcgt ggtgtcgggc
     3601 tcgcggggga aggtggcttc cgcgctcggc ctcgacgtcg tggtggacga ccggcccgag
     3661 aactgcctcg acgtgaaggt cgactcgacg gcgcgggcgg tgctcgtcgc ccgtgccggc
     3721 agccccgcga tggcggcgaa cgccgcacgg ctgggaatcg aggcggtcag ctcgatcgcc
     3781 gagctgctcg accgcctcgg tcgccgcgcg gctgacggcc cgtcgctgct cggccgcatc
     3841 aagaacctgt tgaccggagc gtaggggcga ccctcgtggt cgcccccaaa cggtcgccgc
     3901 cacgtgcagg gcgaccacaa gggtcgcccc tacggctttc tcagccgcgg cagcttcgtc
     3961 ttgtgaggcg gggcggtgcg ggcgcgctcg ggtggatcgg gcaccacctc gatgcgctcg
     4021 ggcgccggct tgggcggcgc ggggttctcg ctcggggccg gagggcggtc ggggccgcgc
     4081 ggccgctcgg gctcaggggt cacagggggc tcgtcggtgc gcggcggagg cggctggtcc
     4141 tggccgcgcc cggcgttccg ttcggcgaac cggtcgggac gcggcttcgc agcgcccgtg
     4201 ccgcggggct tccattcgcc cttcggcctc cactccccct tgggttgctc gcccttcggt
     4261 ttccactcac ccttgggtcg ccacggttcc ttcgggcgcc actcggtctt cgctccccgc
     4321 ggcttcatct cgccgcgcgg ccggtcgtgg ccctggcggt cgccgccccg cttctcgcct
     4381 cccggtggcg gctgctgcgg cgggcggcct tcccgcgggc gttccggctt ccacggccgc
     4441 tggtgcgtcg ggcggccttc ccgcgggcgc tccggtttcc acggccgctc gtgtggcaag
     4501 cggccttcac gtgggcgctc cggcttcgag ggccgctcct gcggcgggcg gccctcgcgc
     4561 ggccgctccg gctttgccgg ccgctcccgc
//
LOCUS       DAOPQX010000307         4589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20453_length_4589_cov_4.968240, whole genome shotgun sequence.
ACCESSION   DAOPQX010000307 DAOPQX010000000
VERSION     DAOPQX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20453_length_4589_cov_4.968240"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="PKK95_13885"
     CDS             complement(<1..1473)
                     /locus_tag="PKK95_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOC19358.1"
                     /translation="MPPESAPAAAFAAPASRVVNDFSIQVATVNGSGSQTANLVLLRS
                     IFQSGVPVSGKNLFPSNIAGLPTWFTIRASRRGYIARRKETDLLVAMNPDTAREDVAS
                     LHPGAAVVYDAPLRLEALRDDVVFYPVPFDRIVAPVCPDARLRKLVRNMIYDGVLARL
                     LGIDLDRMRQALGRQLGKKPKAVALNEAALDAGFEYAAANLEKRDPFVVEPMDETRGR
                     ILVDGNAAAAIGAMMAGVTVVTWYPITPSSSLCEALTGYMRAHRTDPATGKATFAIVQ
                     AEDELSAIGMALGAGWAGARAMTATSGPGISLMAEFTGLGYFAEVPAVIFDIQRAGPS
                     TGLPTRTAQGDLLSTAVLSHGDTRHPMLLPASVSEAYEMAMAAFDLAERLQTPVFVMS
                     DLDLGMNVWMSEPFAYPERPPDRGKRLTPEVLVRLGEWERYRDVDGDGIPWRTVPADG
                     MPAYFCRGSGHNERGQYSERPGDYAGTLERLARKLDTARAL"
     gene            complement(1513..2388)
                     /gene="sucD"
                     /locus_tag="PKK95_13890"
     CDS             complement(1513..2388)
                     /gene="sucD"
                     /locus_tag="PKK95_13890"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOC19359.1"
                     /translation="MAVLIDRSTRLLVQGLTGREGTFHARQAAGYGTAIVGGVVPGKG
                     GTTHEGWPIFNTVADAVSATGANASLIFVPAPSGAETIMEAAESGLGVVVCITEGIPT
                     LDMMRAAAFLRGRPTRLVGPNCPGVISPGKAKAGIIPGHICREGRIGIVSRSGTLTYE
                     AIKQTTLLGFGQSTCIGIGGDPIVGTTFVDALRLFRDDPETDAVVMIGEIGGSAEEEA
                     AAFIAREFPKPVVSFIAGQTAPPGRRMGHAGAIISGGKGTAAEKMDALARAGVRVVKS
                     PAGIGAAVREALAAR"
     gene            complement(2415..3575)
                     /gene="sucC"
                     /locus_tag="PKK95_13895"
     CDS             complement(2415..3575)
                     /gene="sucC"
                     /locus_tag="PKK95_13895"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOC19360.1"
                     /translation="MKIHEFQAKALLAARGVPVPRGEVIQASADAAAAARRLGGTVVV
                     KAQIHAGGRGKGGGVKLARTPEEAERAANEILGMRLVTHQTGPEGRIVKRVLVEEGLE
                     IARELYLGVVIDRAAERPVVMASPAGGMEIERVAAETPGLIFKEHAEPGVGLALFQAR
                     RLGFALGLDWPAVRKASDLIAAVYRTFIEADASLVEINPLVLTGSGGLLALDAKMNLD
                     DNALYRHPDLAALRDLDEEEPLEVEASRHSLNYIRLDGTIGCMVNGAGLAMATMDIIK
                     LAGGEPANFLDVGGGANAEQIRNAFRILTMDRNVRAVLINIFGGILRCDVLAEGLIAA
                     VRGLDVRLPIVIRMEGTNVEIGKRMLHESGLAFTTADTMDEAAREVVALAAR"
     gene            3745..4419
                     /locus_tag="PKK95_13900"
     CDS             3745..4419
                     /locus_tag="PKK95_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOC19361.1"
                     /translation="MASRSKPLSSSVVEKFLIAVTGLVLVLYLVVHLAGNLLLFLGPA
                     RFNAYAHALISNPLVVPAEIGLLAIFVVHVYKTVVMWWHNRAARPQGYYHKEWAGGPS
                     RKSWASTTMIYTGALTALFVVLHVRSFKYGTYYEVAGSGIRDLHRLVIEFFQSPVNVI
                     FYEACLVFLGFHLWHGFASAFESLGADRPGFAPRLLLASRLVAIVIAGGFLAIPLWVY
                     FFGGRV"
BASE COUNT          608 a         1663 c         1604 g          714 t
ORIGIN      
        1 gagcgcgcgg gcggtgtcca gcttccgcgc gagccgctcg agcgtccccg cgtagtcgcc
       61 cggccgctcg ctgtactgtc cccgttcgtt gtgccccgac ccgcggcaga agtaggcggg
      121 catcccgtcg gcggggaccg tccgccacgg gatgccgtcg ccgtccacgt cgcggtagcg
      181 ctcccattcc ccgagccgca ccagcacctc gggggtcagc cgtttcccgc ggtcgggcgg
      241 ccgctccgga tacgcgaacg gctccgacat ccacacgttc atcccgagat cgagatcgct
      301 catcacgaac accggcgtct gaaggcgctc ggcgaggtcg aacgccgcca tcgccatctc
      361 gtaggcctcg ctcaccgagg ctggcagcag catcgggtgc cgggtgtcgc cgtgcgagag
      421 cacggcggtc gagagcaggt cgccctgggc ggtccgcgtg ggcagccccg tcgacggccc
      481 ggctcgctgg atgtcgaaga tcacggccgg cacttccgcg aagtagccga ggccggtgaa
      541 ctcggccatc agcgagatgc cggggcccga ggtggcggtc atcgcccggg cgccggccca
      601 gcccgcgccg agcgccatgc cgatcgcgct caactcgtcc tcggcctgga cgatcgcgaa
      661 ggtggccttc ccggtggcgg ggtcggtgcg gtgggcccgc atgtaccccg tcagcgcctc
      721 gcagaggctc gaggagggcg tgatgggata ccacgtgacg acggtgacgc ccgccatcat
      781 cgcgccgatg gccgcggccg cgttcccgtc gaccaggatc ctgccgcgcg tctcgtccat
      841 cggctcgacg acgaacggat cgcgcttctc gaggttggcc gcggcgtact cgaacccggc
      901 gtcgagcgcc gcctcgttga gcgcgaccgc cttgggcttc ttccccagct gccggccgag
      961 cgcctgccgc atccggtcga ggtcgatgcc gagcagccgc gccagcaccc cgtcgtagat
     1021 catgttccgg acgagcttgc gcaggcgcgc gtcggggcag accggggcga cgatccggtc
     1081 gaacggcacg ggatagaaca ccacgtcgtc gcgcagggcc tcgaggcgca gcggggcgtc
     1141 gtacacgacg gcggcgccgg gatgcaggct ggccacgtcc tcgcgcgccg tgtcggggtt
     1201 catcgcgacg aggaggtcgg tctccttgcg ccgggcgatg taaccccggc ggctcgcccg
     1261 gatcgtgaac caggttggaa ggccggcgat gttcgacggg aagaggttct tgcccgaaac
     1321 ggggacgccg ctctggaaga tcgagcggag cagcaccagg ttcgccgtct ggctgcccga
     1381 gccgttgacg gtggcgacct ggatgctgaa gtcgttgacg acacgcgagg cgggcgcggc
     1441 gaaagccgcc gcgggcgctg actctggggg catacgccga tcgtagcacg cgggccggct
     1501 ttgaaaaccg ggctaccggg cggcgagcgc ctcgcggacg gcggctccga ttccggccgg
     1561 gctcttcacc acgcgcaccc cggcccgcgc gagcgcgtcc atcttctcgg ccgcggttcc
     1621 cttccctccc gagatgatcg cccccgcgtg ccccatgcgc cggccgggcg gggccgtctg
     1681 gccggcgatg aagctgacga ccggcttcgg gaactcgcgc gcgatgaacg ccgccgcctc
     1741 ttcctcggcg ctgcccccga tctcgccgat catcacgacc gcgtccgtct ccggatcgtc
     1801 cctgaacagg cgcagcgcgt ccacgaaggt ggtgccgacg atcgggtcgc ccccgatccc
     1861 gatgcaggtg ctctggccga agccgagcag ggtcgtctgc ttgatcgcct cgtaggtgag
     1921 ggtgccgctg cgcgagacga tgccgatgcg tccctcgcgg cagatgtggc cggggatgat
     1981 gcccgccttg gccttgcccg gtgagatgac gcccgggcag ttcggcccca ccagccgggt
     2041 cggcctgccg cgcaggaagg cggcggcccg catcatgtcg agcgtcggga tgccctcggt
     2101 gatgcagacc accacgccga gccccgactc ggccgcctcc atgatcgtct cggcgcccga
     2161 gggcgccggc acgaagatca gcgaggcgtt ggcgccggtc gcgctcacgg cgtcggccac
     2221 ggtgttgaag atcggccagc cctcgtgcgt ggtgcccccc ttgccgggaa cgaccccgcc
     2281 gacaatggcc gtgccgtacc cggccgcctg ccgcgcgtgg aacgtcccct cgcggccggt
     2341 gagcccctgc accagcaggc gcgtcgaacg atcgatgaga accgccatcg gctcgatgcc
     2401 ctccgggacg cgattcagcg ggccgccagc gcgacgacct cgcgcgcggc ctcgtccatg
     2461 gtgtcggccg tggtgaacgc caggccgctc tcgtgcagca tccgcttccc gatctcgacg
     2521 ttggtgcctt ccatccggat cacgatcggc aggcgcacgt cgagtccccg cacggcggca
     2581 atcagcccct cggcgagcac gtcgcagcgc aggatgccgc cgaagatgtt gatcagcacg
     2641 gcccggacgt tccggtccat ggtgaggatc cggaacgcgt tccggatctg ctcggcgttg
     2701 gcgccgccgc cgacgtcgag gaagttggcg ggctcgccgc ccgccagctt gatgatgtcc
     2761 atcgtcgcca tggccagccc cgcgccgttg accatgcagc cgatcgtgcc gtcgagccgg
     2821 atgtagttga gcgagtgccg cgaggcctcg acctcgagcg gctcctcctc gtccaggtcg
     2881 cgcagggccg cgaggtcggg gtggcggtag agggcgttgt cgtcgaggtt catcttcgcg
     2941 tcgagcgcga ggaggccgcc cgagccggtg aggacgaggg ggttgatctc caccagcgac
     3001 gcgtcggcct cgatgaaggt ccggtagacc gccgcgatca gatccgacgc cttgcgcacg
     3061 gcgggccagt cgagcccgag ggcgaaaccg aggcggcggg cctggaagag ggccaggccg
     3121 acgccgggct ccgcgtgctc cttgaagatc aggcccggcg tctcggcggc gacccgctcg
     3181 atctccatgc cgccggcggg gctggccatc accaccgggc gctcggccgc gcgatcgatg
     3241 acgaccccga ggtacagctc gcgcgcgatc tcgagcccct cctccaccag cacccgcttg
     3301 acgatccggc cctcggggcc cgtctggtgg gtgaccagcc gcatgccgag gatctcgttg
     3361 gccgcccgct cggcctcctc gggcgtgcgg gccagcttca cgccgccgcc cttgcccctg
     3421 ccgcccgcgt ggatctgcgc cttgacgacg acggtgcctc cgagccggcg cgcggccgcc
     3481 gccgcgtcgg ccgaggcctg gatcacctcg ccgcgcggca ccggtacgcc gcgtgcggcg
     3541 aggagggcct tcgcctggaa ttcgtggatc ttcacaggaa gtcatgctgt catgcgccgc
     3601 ggtcctcgtc aattcgtttc accagccggg cgccgcttca cgcgcgcatc tcgccgcggt
     3661 gcggccaccc cgcttgacaa tgggcctctc ggggcaacta gaatgccacc gggcacgaca
     3721 ccgcactcct tggagcacac tgcgatggcc tcgcgctcga agccgctgtc ctcgtcggtc
     3781 gtggagaagt tcctcatcgc cgtgaccggg ctcgtgctcg tgctctacct ggtcgtccac
     3841 ctggccggga acctgctgct cttcctcggt cccgcgcgct tcaacgccta cgcgcacgcg
     3901 ctgatctcga acccgctggt cgtgccggcc gagatcggcc tgctggccat tttcgtggtg
     3961 cacgtctaca agaccgtcgt gatgtggtgg cacaaccggg ccgccaggcc ccaggggtac
     4021 taccacaagg agtgggcggg cgggccgagc cgcaagtcgt gggcctcgac gacgatgatc
     4081 tacaccggcg cgctgaccgc gttgttcgtc gtgctgcacg tccggtcgtt caagtacggg
     4141 acgtactacg aggtggccgg ctccgggatc cgcgacctcc accggctcgt gatcgagttc
     4201 ttccagtcgc cggtgaacgt gatcttctac gaggcctgcc tggtgttcct ggggttccac
     4261 ctctggcacg gctttgcgag cgccttcgag tcgctcggcg ccgaccggcc cgggttcgcg
     4321 ccgcggctgc tgctcgccag caggctggtg gccatcgtga tcgccggcgg gttcctcgcg
     4381 atcccgctgt gggtgtactt cttcggaggc cgagtatgac gccgcccgcg gccggctccg
     4441 ccccgccgac ctcccgcctg gatgcccgcg ttccggccgg gccgatttcc gggaaatggg
     4501 accggcacca gttcgagctc aagctggtga gcccggcgaa ccgccgccgc ttcacggtca
     4561 ttgtcgtcgg caccggcctc gctggaggc
//
LOCUS       DAOPQX010000308         4587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20460_length_4587_cov_4.475728, whole genome shotgun sequence.
ACCESSION   DAOPQX010000308 DAOPQX010000000
VERSION     DAOPQX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20460_length_4587_cov_4.475728"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKK95_13905"
     CDS             <1..324
                     /locus_tag="PKK95_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOC19362.1"
                     /translation="ELKGLAHPASVDTIPTSANKEDHVSMSMGAALKADRALDLARHV
                     LAVELLCACQAIEMRAPLRTSPPLARLLARVREAVPPLVDDRPPSPDIAAVAGLIESG
                     ALDEI"
     gene            483..2168
                     /gene="hutU"
                     /locus_tag="PKK95_13910"
     CDS             483..2168
                     /gene="hutU"
                     /locus_tag="PKK95_13910"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOC19363.1"
                     /translation="MTTASRHVRAPRGPAISCKGWPQEAALRMLMNNLDPEVAERPDD
                     LIVYGGSGKAARNWECFEAITRALRALEHDETLLVQSGKPVGLFRTHPGAPRVLIANA
                     LLVPAWATWENFRDLEHKGLTMYGQMTAGSWIYIGTQGILQGTFETLASLAARRFDGS
                     LEGRLFVSAGLGGMGGAQPLAATMNGGAALVVEVDPHRIERRLQTRYVDEATTSLDEA
                     LEKVSGWQHEKRARSIALLGNAADVIPELAKRGVVPDVLTDQTSAHDPLNGYVPNGLT
                     LEEAQRLRADRPGEYVERAVAAIGRHVAAMLELQRRGSVTFDYGNNIRAQAVKAGVAN
                     AFDIPGFVPEYIRPLFCQGKGPFRWAALSGDPADIHATDRAALEMFRDNAALCRWIGL
                     AGERVAFQGLPARIFWLGYGERARFGLRINEMVRTGEISAPIVIGRDHLDTGSVASPN
                     RETEGMRDGSDAIADWPILNALLNAASGATWVSVHHGGGVGIGYSLHAGMVIVADGSR
                     EADEKLERVLTCDPGIGVARHADAGYPEAIATAEARGVRIPMREFGSAAGGAK"
     gene            2165..3208
                     /locus_tag="PKK95_13915"
     CDS             2165..3208
                     /locus_tag="PKK95_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19364.1"
                     /translation="MNLAPPTEVCERLEARLAGDPPEIPVLVGLCGSGRTTVLRRMQE
                     RLGHERCQYVDVERSATTPERFVQALRTRSPFACAPIERKATARGAFDEARAFLAGAR
                     TPGGQPATFLLDEVLEFRTFESFPGLRRALPELLATLGATPNRFVLASRFPSRAARAV
                     QASAAPFVLTQVDGVGRHEVERMMGAPAPGAAAPVLDQVCLGRPVLVRAVVETMARLP
                     GTPLDPLAAFSAALEPDGAVSKHCRYCYEMRLNRARGYGALKAILDILAGEEPLTLTQ
                     VSRRLGRTPGSTKDYLSWLEDVDLVAVDPRKRYRFRDPVLRLWARLHCRACPPSPEEI
                     AAEVREYAAAVMA"
     gene            complement(3224..4207)
                     /gene="rnc"
                     /locus_tag="PKK95_13920"
     CDS             complement(3224..4207)
                     /gene="rnc"
                     /locus_tag="PKK95_13920"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOC19365.1"
                     /translation="MCPDERERDEASGPLAVVFRTQSDIEASIVRGLLEAHGIYSSLS
                     SDLPHSVFPLTIDGLGEVRISVPAAQAEEAREIIRQQARPGLAAVEPPEPAEARDLSA
                     LEDRLGHRFADPHLLDRALTHRSLANEDATGATGDNESLEFLGDAVLGFIIADLLFHD
                     FPRFDEGQKSKIKSSLVSTTALAQQARKLGLGEFLALGKGEEKTGGRQKFALLADAFE
                     AVLAAIYLDGGLEAARAFVSREFTEAFEDVRSPGFWGRDYKSALQELVQARNLPLPEY
                     GVVAESGPDHRKTFLVEVRVMGELLGEARGTSKKVAEQEAARLAIARLGGQ"
     gene            complement(4197..>4587)
                     /locus_tag="PKK95_13925"
     CDS             complement(4197..>4587)
                     /locus_tag="PKK95_13925"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOC19366.1"
                     /translation="RTANGGRPDVRILTPDGLVLGLRIDSGELFERLLKEESIPLRAG
                     DLLLFFTDGISEAMNGESQMFGEARLGQLVAEHGDLPAGELRERMLREIDAFVAGAPQ
                     HDDMTMILMKFDDFVPGGVRRGLEHVS"
BASE COUNT          619 a         1727 c         1582 g          659 t
ORIGIN      
        1 gagctgaagg gtctcgcgca tccggccagc gtcgacacca ttccaacctc cgccaacaag
       61 gaagaccacg tgagcatgag catgggggcg gccctcaagg ccgaccgcgc gctcgacctc
      121 gcgcgccacg tcctcgccgt ggaactgctc tgcgcgtgcc aggcgatcga gatgcgcgcg
      181 ccgctgcgca cctcgccgcc gctggcgcgc ctgctcgccc gcgtgcgcga ggccgtgccg
      241 ccgctcgtcg acgatcgtcc cccctcgccc gacattgcag cggtggcggg gctgatcgag
      301 tcgggcgcgc tcgacgagat ctgacgcgac ctttgtgtcc tttgtggttc aaccctgttg
      361 ttcttcgtgt tcctggtgat ttcatcttcg tgatcgtcgt ggtctgtctt gacaaccacc
      421 gcccgcctct ctaagtttct gatctgccgc agcccctggc cgcgagcccg caaggaccac
      481 ccatgactac cgcgtcgcgc cacgtccgcg ctccccgcgg ccccgccatc tcctgcaagg
      541 ggtggccgca ggaggccgcg ctccgcatgc tgatgaacaa cctcgacccg gaggtggccg
      601 agcggcccga cgatttgatc gtctacggcg ggtcgggcaa ggcggcccgc aactgggagt
      661 gcttcgaggc gatcacccgc gcgctgcgcg ccctcgagca cgacgaaacc ctgctcgtac
      721 agtcgggaaa gccggtcggc ctgttccgca cgcaccccgg cgccccgcgc gtgctcatcg
      781 ccaacgccct gctcgtgccc gcgtgggcca cctgggagaa cttccgcgac ctcgagcaca
      841 agggcctgac gatgtacggc cagatgacgg ccggcagctg gatctacatc ggcacgcagg
      901 gcatcctgca gggcaccttc gagacgctcg cttcgctggc cgcccgccgc ttcgacggca
      961 gcctcgaggg ccgccttttc gtgtccgcgg ggctcggcgg gatgggcggc gcccagccgc
     1021 tcgcggccac gatgaacggc ggcgccgccc tcgtcgtcga ggtggacccg caccggatcg
     1081 agcggcgcct ccagacccgt tacgtggacg aggccacgac ctcgctcgac gaggcgctgg
     1141 agaaggtgtc ggggtggcag cacgagaagc gcgcccgctc gatcgcgctg ctgggcaacg
     1201 ccgccgacgt catccccgag ctggcgaagc gcggcgtcgt tcccgacgtg ctgaccgacc
     1261 agacctccgc gcacgacccg ctgaacggct acgtgcccaa cggcctcacc ctcgaggagg
     1321 cccagcgcct gcgcgccgac cggcccggcg agtacgtgga gcgcgcggtt gccgccatcg
     1381 gccgccacgt cgccgcgatg ctggagttgc agcggcgcgg cagcgtgacg ttcgactacg
     1441 gcaacaacat tcgcgcgcag gccgtgaagg cgggcgttgc gaacgccttc gacatcccgg
     1501 gcttcgtgcc cgagtacatc cggccgctct tctgccaggg caaggggccg ttccgctggg
     1561 ccgcgctgtc gggcgacccg gccgacatcc acgccaccga ccgcgcggcg ctcgagatgt
     1621 tccgcgacaa cgccgccctc tgccgctgga tcggccttgc cggcgagcgc gtcgcgttcc
     1681 aggggctgcc ggcgcggatc ttctggctcg gctacggcga gcgcgcgcgc ttcggcctgc
     1741 gtatcaacga gatggtgcgc acgggcgaga tctcggcgcc gatcgtcatc ggccgcgatc
     1801 acctcgacac cggctcggtg gcctcgccca accgcgaaac cgagggaatg cgggacggga
     1861 gcgacgcgat cgccgactgg cccatcctca acgccctgct caacgcggcg tcgggggcga
     1921 cctgggtgtc ggtgcaccac ggcggcggcg tggggatcgg ctactcgctg cacgccggca
     1981 tggtgatcgt ggccgacggc tcccgcgagg ccgacgagaa gctggagcgg gtgctgacct
     2041 gcgacccggg aatcggcgtc gcccgccacg cggatgccgg ctaccccgag gcgattgcga
     2101 cggccgaggc gcgcggcgtg cgcatcccga tgcgcgagtt cggcagcgcg gctggcggtg
     2161 cgaagtgaac ctggcgcccc cgaccgaggt ctgcgagcgg ctcgaggcac gcctggcggg
     2221 cgacccgccc gagatccccg tcctcgttgg cctctgcggc agcgggcgca cgaccgtgct
     2281 gcgccgcatg caggagcgcc tcgggcacga acgctgccag tacgtggacg tggagcggag
     2341 cgccaccacc cccgagcgct tcgtccaggc gctgcgcacc cgatcgccct tcgcctgcgc
     2401 accgatcgag cgcaaggcca cggcgcgcgg cgccttcgac gaggcgcggg cgttcctcgc
     2461 gggggcgcgg acgcccggcg gccagccggc caccttcctg ctcgacgagg tgctcgagtt
     2521 ccgcaccttc gagagcttcc cgggcttgcg gcgcgccttg cccgaactgc tggcaacgct
     2581 cggcgccacc cccaaccggt tcgtgctggc ctcgaggttc cccagccgcg ccgcgcgcgc
     2641 cgtgcaggcc tcggccgccc cgttcgtcct cacgcaggtg gacggcgtcg ggcggcatga
     2701 ggtggagcgg atgatgggcg cccccgcgcc gggggcggcc gcccccgtgc tcgaccaggt
     2761 gtgcctggga cggcctgtcc tcgtccgcgc cgtggtggaa acgatggcgc gcctgccggg
     2821 cacgccgctc gacccgctcg ccgcgttctc cgccgcgctc gagcccgatg gcgccgtctc
     2881 gaaacactgc cgctactgct acgagatgcg cctgaaccgc gcgcgcggct acggcgccct
     2941 caaggcgatc ctcgacatcc tcgccggcga ggagccgctc accctgacgc aggtgtcgcg
     3001 ccggctggga cgcacgccgg gctcgacaaa ggactacctg tcgtggctgg aagacgtgga
     3061 cctggtcgcg gtggacccgc gcaagcggta ccgcttccgc gacccggtgc tgcggctctg
     3121 ggcgcggctg cactgccgcg cgtgtccgcc ttcgcccgag gagatcgccg ccgaggtgag
     3181 ggagtacgcg gctgccgtga tggcctagcc ggggcgccga attctactgc ccgcccaggc
     3241 gcgcgatcgc cagccgcgcg gcctcctgtt cggccacctt cttgctggtg cctcgcgcct
     3301 ccccgagcag ctcgcccatc acgcgcacct cgacgaggaa cgtcttgcgg tggtcggggc
     3361 cgctctcggc cacgacgccg tactcgggga gcggcaggtt gcgcgcctgg accagctcct
     3421 gcagcgccga cttgtagtcg cggccccaga acccggggct ccggacgtcc tcgaaggcct
     3481 cggtgaactc gcgtgacacg aaggctcgcg cggcctcgag ccccccgtcg aggtagatgg
     3541 ccgcgagcac cgcctcgaag gcgtccgcca gcagcgcgaa cttctggcgc ccgcccgtct
     3601 tctcctcacc cttcccgagg gcgaggaact ccccgaggcc aagcttcctc gcctgctgcg
     3661 ccagcgccgt cgtcgagacg agcgacgact tgatcttcga cttctgcccc tcgtcgaacc
     3721 gcgggaagtc gtggaagagc aggtcggcga tgatgaaccc gagcaccgcg tcgcccagga
     3781 actcgagcga ctcgttgtcg ccggtcgccc cggtcgcgtc ctcgttggcc agggaccggt
     3841 gggtcagcgc gcggtcgagc aggtgcgggt cggcgaagcg gtgcccgagc cgatcctcga
     3901 gcgccgagag gtcgcgcgcc tcggccggct cgggcggctc caccgcggcg agccccggcc
     3961 gcgcctgctg ccggatgatc tcgcgcgcct cctccgcctg cgccgccggc accgagatcc
     4021 gcacctctcc cagcccgtcg atcgtcagcg ggaagaccga gtgcgggagg tcggacgaga
     4081 gcgacgagta gatgccatgg gcctcgagca gcccgcgcac gatgctcgcc tcgatgtccg
     4141 actgcgtgcg gaagacgacg gcgagcgggc ccgaagcctc gtcgcgttcg cgctcgtcag
     4201 gacacatgtt cgagccccct gcgcaccccg ccgggcacga agtcgtcgaa cttcatcagg
     4261 atcatggtca tgtcgtcgtg ctgcggcgcg ccggcgacga aggcgtcgat ctcgcgcagc
     4321 atccgctcgc gcagctcgcc cgccggcagg tcgccatgct cggccaccag ctggccgagc
     4381 cgcgcttcgc cgaacatctg cgactcgccg ttcatcgcct cgctgatccc gtcggtgaag
     4441 aacagcagca ggtcgcccgc ccggagcggg atcgattcct ccttcagcag ccgctcgaac
     4501 agctcgccgc tgtcgatccg caggccgagc accagcccgt ccggcgtcag gatgcggacg
     4561 tcgggccgcc cgccgttcgc cgtccgg
//
LOCUS       DAOPQX010000309         4571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20552_length_4571_cov_3.899911, whole genome shotgun sequence.
ACCESSION   DAOPQX010000309 DAOPQX010000000
VERSION     DAOPQX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20552_length_4571_cov_3.899911"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2221
                     /locus_tag="PKK95_13930"
     CDS             <1..2221
                     /locus_tag="PKK95_13930"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC19367.1"
                     /translation="VQVSVTRNYGETAEHKSYELLWHMFLAVFSVSILIWLTLGRREA
                     AVVLIAIPVTLALTLFVFYLYGYTLNRVTLFALIFSIGILVDDAIVVVENIVRHARLA
                     GPGSRLASVAVKAVDEVGNPTILATLTVVAAILPMAMVGGLMGPYMRPIPVGATAAMM
                     FSLVVAFVVTPWAAARLMKTPSGHAQGAEDRLTVVYRRVMNRLIARPRYRAAFLATVA
                     LLLLGAAALVPLGLVKVKMLPFDNKSEFQVIVNTDEGTPLEQTARIAAALASETLRQP
                     EVLNVQAYVGTASPYNFNGLVRHYFMRRVPHQADLQVNLVDKDERSAQSHDIAKRVRT
                     ALEPIAKRYGARVQVAEVPPGPPVLQTLVAEVYGPDESRRLEIATRVKKTFEETAGVV
                     DADWYVEHPQRKDRLEVDHEKAAAAGVSAASIASVVRLAGSGQTVGLLHDERAREDVP
                     IVVRLPRALRSSTDALQAIRLGGSSPVAIGELTRVARVEENRSIYHKNLRPVTYVMAD
                     VAGTVESPVYAILAMNKVLSRLQLPEGYRFEIYNARQPHDSNKYAMKWDGEWHITYEV
                     FRDLGLAFAAVLLLIYILVVGWFQSFWTPIGIMAAIPFSLVGILPAHAMLGAFFTATS
                     MIGFIAGAGIVVRNSIILVDFIELRLRDGMPLAEAVVDAGAVRFRPMALTAAAVVVGA
                     GVILFDPIFQGLAISLMAGEIASLLLSRATVPVLYYSMNRRAAEGGTLQRAIAPAGE"
     gene            2299..2487
                     /locus_tag="PKK95_13935"
     CDS             2299..2487
                     /locus_tag="PKK95_13935"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOC19368.1"
                     /translation="MTVERYLRLIAGLFVLASVLLGMYAHPAFFWFTAFVGLNLFQSA
                     FTGWCPMMAILRKAGVRG"
     gene            2535..2948
                     /locus_tag="PKK95_13940"
     CDS             2535..2948
                     /locus_tag="PKK95_13940"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOC19369.1"
                     /translation="MQETHYGLRVELPIGYDEAVERATSALKAEGFGVLTTIDVKATM
                     KQKLDADFRRYVILGACNPPLAHQALTAELEIGLLLPCNVIVYETGPGASVVAAMAPL
                     GAMSVVGNPALTPVAQQADGKLRKALAALEQGQAA"
     gene            complement(3120..3380)
                     /locus_tag="PKK95_13945"
     CDS             complement(3120..3380)
                     /locus_tag="PKK95_13945"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOC19370.1"
                     /translation="MSSSSSASRSERGTRLSHLKAGERGVVVGVLRDDPARAERLGAL
                     GVTPGARIRLLQRFPGFVFECDQTEIVVEPAVARAILVEVER"
     gene            complement(3349..>4571)
                     /locus_tag="PKK95_13950"
     CDS             complement(3349..>4571)
                     /locus_tag="PKK95_13950"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOC19371.1"
                     /translation="AGAADPARWAHETAERLRRLRPSSAGRVQAWLGQALRRPLTGLP
                     VLALVLYAVYLVVGVFGAQTLVGLIEEGVFGRYVNPAATAFLTWAVPFALVRDFLVGE
                     YGLITMGLTYAVAIVLPVVATFFLVFSWLEDSGYIPRLAIFSDRIFRMMGLNGKAVLP
                     MVLGLGCDTMATMTTRILGTPKERVIAVLLLALGVPCSAQLATILGILGGISFAALAT
                     LAAVVLGQMFLVGFLAARVLPGERSDFILELPPMRWPSARNVVVKTAIRLRWYLAEAV
                     PLFLIGTALLFVLDRTGGLDVIIAAGRPVVSGLLGLPAETAQVFVMGFLRRDYGAAGL
                     FQLAHAGQLTGVQAVVALTVMTLFVPCVAHFLMIVREQGARVAAAILLFVTAVALGTG
                     AGLHHVLQFIGISF"
BASE COUNT          736 a         1559 c         1598 g          678 t
ORIGIN      
        1 cgtgcaggtc tcggtgacgc gcaactacgg ggagaccgcc gagcacaagt cgtacgagct
       61 gctctggcac atgttcctgg cggtcttctc ggtgtcgatc ctgatctggc tcacgctcgg
      121 ccggcgcgag gcggccgtcg tgttgatcgc gatcccggtg acccttgccc tgacgctgtt
      181 cgtcttctac ctctacggct acacgctgaa ccgcgtgacg ctgttcgcgc tgatcttctc
      241 gatcggcatc ctggtggacg acgcgatcgt cgtcgtcgag aacatcgtgc gccacgcccg
      301 gctcgccggc ccgggaagcc ggctcgcgtc ggttgcagtg aaagcggtgg acgaggtcgg
      361 caaccccacg atcctggcga cgctcaccgt cgtcgccgcg attctcccca tggcgatggt
      421 cggcgggctg atgggcccct acatgcggcc gattcccgtc ggggccacgg ccgcgatgat
      481 gttctcgctc gtggtggcct tcgtggtgac gccctgggcc gctgcgcggc tgatgaagac
      541 gccgtcgggc cacgcccagg gcgctgagga tcgcctcacg gtcgtctacc gccgcgtgat
      601 gaaccggctc atcgcgcggc cgcggtaccg ggcggccttc ctggcgacgg tcgccctgct
      661 gctgctcggg gcggccgcgc tggtgccgct cggcctcgtc aaggtgaaga tgctgccgtt
      721 cgacaacaag agcgagttcc aggtgatcgt gaacaccgac gagggaacgc cgctcgagca
      781 gacggcacgc atcgccgcgg ccctggccag cgaaacgctc cggcagccgg aggtgctgaa
      841 cgtgcaggcg tacgtcggca ccgcctcgcc gtacaacttc aacggcctcg tccgccacta
      901 cttcatgcgg cgggtgcccc accaggccga cctgcaggtg aacctggtcg acaaggacga
      961 gcggtcggcg cagagccacg acatcgccaa gcgcgtccga accgcgctcg agccgattgc
     1021 gaagcggtat ggcgcgcgcg tgcaggtggc cgaggtgccg cccggcccgc ccgtgctgca
     1081 gacgctcgtc gccgaggtgt acgggccgga cgagtcgcgg cggctggaga tcgccacgcg
     1141 ggtgaagaag accttcgaag agacggcggg cgtcgtggac gcggactggt acgtcgagca
     1201 cccgcagcgg aaggaccggc tcgaggtgga ccacgagaag gcggccgcgg caggagtctc
     1261 ggctgcctcg atcgcctcgg tggtgcggct ggccggctcg gggcagaccg tgggcctgct
     1321 ccacgacgaa cgggcccgcg aggacgttcc gatcgtggtg cgcctgccgc gcgccctccg
     1381 ctcgtccacc gacgcgctgc aggccatccg gctcggcggt tcgtcgcccg tggcaatcgg
     1441 tgagctgacc cgcgtggcga gggtggagga gaatcgcagc atctaccaca agaacctgag
     1501 gcccgtgact tacgtcatgg ccgacgtcgc cggcacggtg gagagcccgg tgtacgccat
     1561 cctggccatg aacaaggtgc tctcgcgcct gcagctgccg gagggctacc ggttcgagat
     1621 ctacaacgcc cggcagcccc acgacagcaa caagtacgcg atgaagtggg acggcgagtg
     1681 gcacatcacc tacgaggtgt tccgcgacct gggcctcgcc ttcgcggccg tgctgctgct
     1741 catctacatc ctcgtcgtgg gatggttcca gtcgttctgg acgccgatcg ggatcatggc
     1801 cgccatcccc ttctcgctgg tcggcatcct gccggcacac gcgatgctgg gggcgttctt
     1861 caccgccacg tcgatgatcg ggttcatcgc cggcgccggc atcgtcgtgc gcaactcgat
     1921 catcctcgtg gacttcatcg agctgcggct gcgcgacggc atgccgttgg ccgaggcggt
     1981 cgtcgatgcc ggtgccgtgc ggttccgccc gatggccctc acggccgccg ccgtcgtcgt
     2041 gggcgccggg gtcatcctgt tcgacccgat cttccagggg ctggccatct cgctgatggc
     2101 cggcgaaatc gcctccctgc tgctctcgcg ggccaccgtg cccgtcctgt actactcgat
     2161 gaaccggcgc gccgccgagg gcgggacgtt acagcgggcc atcgcgccgg ccggggagtg
     2221 agacggcgcc gatgcttttc gtacctgaac cgtgaatcct gaaccccgat tcctgaatcc
     2281 tgtgaggagg aggcgcccat gaccgtcgaa cgctacctcc ggctcatcgc cgggttgttc
     2341 gtgctggcca gcgtcctgct gggaatgtac gcccaccccg ccttcttctg gttcacggcg
     2401 ttcgtgggcc tgaacctgtt ccagtcggcc ttcacgggct ggtgcccgat gatggcgatc
     2461 ttgagaaagg ccggcgtgag gggctgaccc cagccgacga aggcactgcg ctagactgga
     2521 gaaacggagg gttcgtgcag gaaacgcact acggcttgcg agtggagctg ccgatcgggt
     2581 acgacgaggc ggtcgagcgc gccacctcgg cactcaaggc cgagggtttc ggcgtgctga
     2641 cgacgatcga cgtcaaggcg acgatgaagc agaagctcga tgccgacttc cgccggtacg
     2701 tcatcctggg ggcctgcaac ccgccgctgg cgcaccaggc actcaccgcg gagctcgaga
     2761 tcggcctgct gctcccgtgc aatgtcatcg tgtacgagac gggccccggc gcgagcgtgg
     2821 tggcggccat ggctccgctc ggcgcgatga gcgtggtcgg caacccggca ctgacccccg
     2881 tggcccagca ggccgatgga aagctgcgga aggcgctcgc cgcgctcgag caggggcagg
     2941 cggcttgatg gactacctct ggccccttct ctttctggtg gtgtgggtgc tgctgcaggt
     3001 cgtcatcctg ccgcgcctcg gcgtgccgac ttgagccagg ccggcctgcg gcgtcgagga
     3061 tcgacgcatc gatcgcaagg atcaggaacg cgagacccgg ctgtaacgcc ggcgccgcgc
     3121 tagcgctcca cctcaaccag gatcgcccgg gctaccgcgg gttcgacgac gatctccgtc
     3181 tggtcgcact cgaacacgaa tcccggaaac cgctggagca agcggatgcg ggcgcccggc
     3241 gtcacgccga gcgcacccag gcgctcggcc ctggccgggt cgtcgcgaag cacgccgacc
     3301 accaccccac gctcgcccgc cttcaggtga gacaggcggg ttccccgctc agaacgagat
     3361 gccgatgaac tggaggacat ggtgaaggcc cgcgccggtg ccaagcgcca ccgcggtgac
     3421 gaacagcagg atggcggcag ccacccttgc cccttgctcg cggacgatca tcaggaagtg
     3481 ggcgacgcag ggcacgaaca gcgtcatcac ggtaagggcg acgacggcct gcacgccggt
     3541 cagttgcccg gcgtgcgcca gctggaacag cccggccgcg ccgtagtcgc gccgcaggaa
     3601 gcccatgacg aacacctgcg cggtctcggc ggggaggcct agcaggccac tgacgaccgg
     3661 ccggccggcc gcgatgatga cgtcgagtcc cccggtcctg tcgaggacga acaacagcgc
     3721 cgtgccgatc aggaagagcg gcacggcctc ggcgagatac cagcgcagcc gaatggccgt
     3781 cttcaccacg acgttcctgg ccgagggcca ccgcatcggc ggcagttcga ggatgaagtc
     3841 ggagcgttcg cccggcagca cgcgcgccgc caggaacccc accaggaaca tctgccccag
     3901 cacgaccgcg gccagagtgg cgagcgccgc gaaagagatc ccgcccagga tcccgaggat
     3961 ggtcgccagc tgggccgagc acgggacgcc gagggccagc agcaggacgg cgatcacccg
     4021 ttccttcggc gttcccagga tgcgcgtggt catcgtcgcc atggtgtcgc agccgaggcc
     4081 cagcaccatc ggcagaaccg ccttcccgtt caggcccatc atgcggaaga tgcggtcgga
     4141 gaagatcgcc aggcgcggga tgtagccgct gtcctcgagc cacgagaaca cgaggaagaa
     4201 ggtggcgacc acgggaagga cgatggcgac cgcgtaggtc aggcccatcg tgatgaggcc
     4261 gtattcaccg acgaggaagt cgcggaccag ggcaaagggg acggcccagg tgaggaacgc
     4321 cgtcgccgcg gggttgacgt accggccgaa gacgccctcc tcgatgaggc ccaccagggt
     4381 ctgcgccccg aagacgccca cgacgaggta gacggcatag aggacgagcg cgaggaccgg
     4441 gaggccggtc aggggccggc gcagcgcctg ccccagccac gcctggaccc ggccggccga
     4501 ggaggggcgc agccggcgaa gccgctccgc cgtctcgtgc gcccagcgcg cggggtcggc
     4561 cgcgccagct g
//
LOCUS       DAOPQX010000310         4539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20720_length_4539_cov_3.972346, whole genome shotgun sequence.
ACCESSION   DAOPQX010000310 DAOPQX010000000
VERSION     DAOPQX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20720_length_4539_cov_3.972346"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKK95_13955"
     CDS             complement(<1..300)
                     /locus_tag="PKK95_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC19372.1"
                     /translation="MRILLADNHRLFCEGLRTLFATRPGLDIVGEANNGRMAVRLARE
                     LSPDLVIMDIGMPELNGVEATRQIRAEMPNVKVLAVSMHSDRQYVAGMLAAGASGY"
     gene            complement(297..1889)
                     /locus_tag="PKK95_13960"
     CDS             complement(297..1889)
                     /locus_tag="PKK95_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOC19373.1"
                     /translation="MRIHTNELGVDLGPAGRARLEAALARAFSEAAPRVHLVNVSLKR
                     SEIHEATVTCVIAAHLVPSGRIVVALRAEGPARAIDRALAELRRELAPHRRAAARNPS
                     AGANSSTQVKLRGELGRMAARSGFKMPVRTSSPGVRRMEKDRKGRRRAPAVAPAAAPP
                     GAEALSARSDALAERVKELKCLHSIWRMLKGHDADLGDILQEVADVLPSACQYPDLAC
                     ASITLKSRRYHTRNFYRPVCCYRAEITVGGRTVGALEVGYSDTLEDGREPALLDEERT
                     LFRMVADYVSEIVALNEAKTQLSTYQEHLRSLAAELAMAEERERRTLAISLHDRIGQG
                     LAVAKLKLETLQYLLPPEHHSRLEDVTALIKEIVNDTRSLTFDISPPILYELGLTPAV
                     VWLGEHVTRQFGLPVEVRCRAEGVVLNEGLRIMLFRSIQELLMNAVKHARASRASVRL
                     FTQGVSEICALVEDDGVGFDPPSCTLHPSAASGFGLFSIRERIGHLGGRVEIESAPGK
                     GTRVRLTVPGTRVKPSSGEAMR"
     gene            2240..2473
                     /locus_tag="PKK95_13965"
     CDS             2240..2473
                     /locus_tag="PKK95_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19374.1"
                     /translation="MRTDELSSRVRGEYLEMPGLSLTLAQAQRLWALDRARCSRLLEH
                     LEETGFLRRRRDGSYVRRQAGVEPWMRATPSQG"
     gene            2488..2811
                     /locus_tag="PKK95_13970"
     CDS             2488..2811
                     /locus_tag="PKK95_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19375.1"
                     /translation="MRTTVQDEGRHFDRQTHAYAEYRVFSRLMGAAGVEGDVTVMVAL
                     KRLDGDAPGAPLVRCEIAVAQRTSEVARVQALGPHPYAAIDRAAVLIGEAVRRQAQRS
                     RATAG"
     gene            complement(2854..4245)
                     /locus_tag="PKK95_13975"
     CDS             complement(2854..4245)
                     /locus_tag="PKK95_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19376.1"
                     /translation="MTGRRFAADQVAPSPDRPLTAGRGKEDSTVIHPAQRALIVGCRG
                     GVGRAVLSLLEHSRPGGRLRSRLEALVLADTRGGSESVSGAVALPPVTIRSAGDLAQI
                     VREHRITQVIALGSLDTIECIEVCDALGADFLSASAEEWPGGASIPTDQVIGRLLPPN
                     APAASRSHLVGSGANPGLVNALAPTALRTFARRVGVAPRADALDLYAVLVTEEDTTRE
                     IGAPSPADVFAMTWSPSHCLEELLEPRAFAVSGGRVVGLGHRPVESAYRARCGARGIN
                     GMAVPHEELLTLSRLFPSVEMAFVYRLPHAARRALRKHPGRVRPEQWRTHRLYPPWAR
                     ALRGEDRLGVLLCSRRFGEFWAGFRTDVADGVALGTNATQLQTAAGVLAGWWQLGRRR
                     GVHFVEDLDVEEYVRSAADILGWPLAVEDPEAPFLPLAERLVPPPPRLRRRAAAPAAG
                     VAPASGVEDLVLN"
     gene            complement(4242..>4539)
                     /locus_tag="PKK95_13980"
     CDS             complement(4242..>4539)
                     /locus_tag="PKK95_13980"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOC19377.1"
                     /translation="LDRAHVVPADSIPADVVTVDSEVSLRNLDTGEEMVFTVVFPGKA
                     NVDERRLSVLAPLGAAVLGYRAGDIIEWTVPGGVRRLKVEEVLYQPEAARKAGE"
BASE COUNT          649 a         1653 c         1612 g          625 t
ORIGIN      
        1 gtagcccgaa gcgccggccg cgagcatgcc cgcgacgtac tggcggtcgg agtgcatcga
       61 gacggcgagc accttcacgt tcggcatctc ggcccggatc tgccgcgtgg cctcgacccc
      121 gttcagctcg ggcatcccga tgtccatgat caccaggtcg ggtgacagct cgcgtgccag
      181 gcggacggcc atccgcccgt tgttggcctc gccgacgatg tcgagacccg gccgggtcgc
      241 gaacagcgtg cgcagcccct cgcagaacag ccggtggttg tcggcgagca ggatcctcat
      301 cgcattgcct ccccgctcga tggtttgacc cgtgtcccgg gaaccgtgag gcgcactcgc
      361 gtccccttcc cgggagccga ctcgatctcc acgcgcccgc caaggtggcc gatccgctcg
      421 cggatgctga acagcccgaa cccgctggcc gccgacgggt gcagcgtgca gcttggcgga
      481 tcgaacccca ccccgtcgtc ttccacgagg gcgcagatct cgctgacgcc ctgcgtgaag
      541 agccggaccg acgcccgcga ggcgcgcgcg tgcttgaccg cgttcatcag cagctcctgg
      601 atcgaccgga acagcatgat gcgcagcccc tcgttcagca cgaccccttc ggcgcggcag
      661 cgcacctcca cgggaagccc gaactgccgg gtgacgtgct cgcccagcca caccaccgcc
      721 ggcgtgagcc ccagctcgta gaggatcggc gggctgatgt cgaaggtcag cgagcgggtg
      781 tcgttcacga tctccttgat gagcgccgtg acgtcctcga gccgcgagtg gtgctcgggc
      841 gggaggaggt actgcagcgt ctcgagcttc agcttcgcca ccgcgagccc ctggccgatc
      901 cgatcgtgga ggctgatggc cagggtgcgc cgctcgcgct cctccgccat cgcgagctcg
      961 gccgcgagcg acctgaggtg ctcctggtag gtggagagct gcgtcttcgc ctcgttcagc
     1021 gccacgatct ccgagacgta gtcggccacc atcctgaaga gcgtccgctc ctcgtcgagc
     1081 agggcgggct cgcgcccgtc ctcgagcgtg tcgctgtagc ccacctccag cgcgcccacc
     1141 gttcgcccgc cgaccgtgat ctccgcgcgg tagcagcaga cgggccggta gaagttgcgc
     1201 gtgtggtagc ggcggctctt cagcgtgatc gacgcgcagg ccaggtcggg atactggcag
     1261 gccgacggca gcacgtcggc tacctcctgc aggatgtcgc cgaggtctgc gtcgtgcccc
     1321 ttcagcatcc gccagatcga gtgaaggcac ttcagctcct tcactcgctc ggccagggca
     1381 tcggaccggg cgctgagcgc ctccgctcct ggcggcgccg cggctggcgc gacggcaggc
     1441 gcccgccgcc tccccttccg atccttctcc atccggcgca ccccgggcga cgacgtccga
     1501 acgggcatct tgaagccact cctggcggcc attctaccca actccccccg gagcttcact
     1561 tgcgttgacg agttggctcc ggcagaagga ttgcgcgcag ccgcgcggcg gtgcggggcc
     1621 agctcgcggc gcaactcggc gagcgcgcgg tcgatggcgc gcgcggggcc ctcggcgcgc
     1681 agcgccacca cgatccgccc cgagggcacc aggtgcgcag caatgacgca ggtgacggtg
     1741 gcctcgtgga tttcgctgcg cttgagcgag acgttcacga gatggacgcg cggggcagcc
     1801 tcgctgaacg cccgcgcgag ggccgcctcg aggcgcgcac gcccggccgg gccaaggtcg
     1861 acgccaagct cgttggtgtg gatgcgcatt gcgaagggcc ggacgccaga gcgagagggg
     1921 cccaaggtcg cggttgaaag actcgtgcca ggaagcgcgg aggcgtaggc ttcaataggg
     1981 tcaaaagaca gggctatgac gctgccttgg cgatccgctc acccacgcag tttcaatgag
     2041 cacacatcac atttcattct gaaatgcccg gcaggggatc cccggccccc cgtgccggct
     2101 ttgggcccgt tctccgcggt aatccggcac tgtgggcgct gcccctgcac ggcccggggt
     2161 ttgcccttcc cgaggattgg ggccgcgccg agccttcatc ccggatcgct cgtgccgagg
     2221 cgccaaggag gacgaacgga tgcgcacgga cgaactttcc tcccgcgtgc ggggggagta
     2281 tctcgagatg cccggtctca gcctgacgct cgcgcaggcg cagcgcctct gggcgctcga
     2341 ccgcgcgcgg tgctcgcgcc tgctggagca cctcgaggag accggtttcc tgcggcgccg
     2401 ccgtgacggt tcctacgtcc ggcggcaggc aggggtggaa ccgtggatgc gggccacgcc
     2461 ctcgcaaggc tagcccggga gcgatacatg cgaaccaccg tccaggacga gggacggcac
     2521 ttcgatcggc agacccacgc gtacgccgaa taccgggtct tctcgagact gatgggcgcg
     2581 gccggcgtgg aaggagacgt cacggtgatg gtcgcgctga agcggctcga tggcgacgcg
     2641 cccggcgccc cgctcgtgcg ctgcgagatc gccgtcgccc agcgcacgag cgaggtggcg
     2701 cgcgtgcagg cgctcgggcc gcatccctac gccgccatcg accgggccgc ggtgctgatc
     2761 ggcgaggccg tgcgccgcca ggctcagcgg agccgcgcca ccgcgggctg aggccgccgg
     2821 cgacgctgac gcagcagtcc ggcgcggcgc gtttcagttg aggacgaggt cctcgacgcc
     2881 cgaagcgggc gccaccccgg cagccggggc ggcggcccgc cgccgcaggc gtggtggggg
     2941 cggcacgagc cgctcggcga gcggcaggaa cggcgcctcg ggatcctcca ccgccagcgg
     3001 ccacccgagg atgtcggccg cggaccgcac gtattcctcg acgtcgaggt cttccacgaa
     3061 gtgcacgccg cgccggcgcc ccagttgcca ccacccggcc agcacgccgg ccgccgtctg
     3121 gagctgggtg gcgttggtgc cgagcgccac gccgtccgcc acgtcggtcc ggaaacccgc
     3181 ccagaactcg ccgaaccgcc ggctgcacag cagcacgccc aaccggtcct cgccccggag
     3241 cgcgcgcgcc cagggcggat agagccggtg cgtccgccac tgctcgggcc gcacccggcc
     3301 ggggtgtttc cgcagcgccc gccgcgcggc gtgcggcagc ctgtagacga acgccatctc
     3361 caccgacggg aagaggcgcg agagcgtgag cagctcctcg tgcgggaccg ccatgccgtt
     3421 gatgccgcgg gctccgcagc gggccctgta ggcggactcc accgggcggt gtcccaggcc
     3481 caccacgcgc ccccccgaca ccgcgaacgc cctcggttcg agcagctcct cgaggcagtg
     3541 gctcgggctc cacgtcatcg cgaacacgtc tgcaggcgag ggggcgccga tctcgcgcgt
     3601 ggtgtcttcc tcggtcacca gcacggcgta caggtcgagg gcgtccgcgc gtggagccac
     3661 ccccacgcgg cgggcgaacg tgcgcagggc ggtcggggcc agcgcattga cgagccccgg
     3721 gttggccccg ctccccacga gatggctgcg cgaggcggcc ggggcgttgg gcggaagcag
     3781 ccgcccgatg acctggtcgg tgggaatcga cgcgccgccg ggccactcct ccgcgctcgc
     3841 ggacaggaag tcggcgccca gcgcgtcgca aacctcgatg cactcgatcg tgtcgagcga
     3901 gccgagcgcg atgacctgcg tgatccggtg ctcgcgaacg atctgcgcca ggtcgccggc
     3961 cgaccggatg gtgacgggcg gcagggcgac cgccccgctc accgactcgg aaccgccccg
     4021 ggtatcggcg agcacgaggg cctcgagacg cgagcggagc cgccccccgg ggcggctgtg
     4081 ttcgagaagc gagagcaccg cgcggccgac gccgccgcgg cagcccacga tgagggcgcg
     4141 ctgagccggg tggatcaccg tgctgtcctc cttgccgcgt ccggcggtga gggggcggtc
     4201 tggcgagggg gcgacctggt cggctgcgaa gcggcggccc gtcattcgcc ggcctttcgg
     4261 gccgcctcgg gctggtacag cacctcttcc accttcaggc gccgcacgcc tcccggcacc
     4321 gtccactcga tgatgtcgcc ggcccggtac ccgagcacgg cggcgccgag cggcgcgagc
     4381 accgacagcc gccgctcgtc gacgttcgcc ttcccgggaa agacgacggt gaacaccatc
     4441 tcctcgccgg tgtcgaggtt ccgcagggac acctcggaat ccacggtgac cacgtcggcc
     4501 gggatgctgt cggccggcac gacgtgcgcg cggtcgagc
//
LOCUS       DAOPQX010000311         4528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20794_length_4528_cov_5.038677, whole genome shotgun sequence.
ACCESSION   DAOPQX010000311 DAOPQX010000000
VERSION     DAOPQX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20794_length_4528_cov_5.038677"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..339)
                     /gene="yedF"
                     /locus_tag="PKK95_13985"
     CDS             complement(1..339)
                     /gene="yedF"
                     /locus_tag="PKK95_13985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOC19378.1"
                     /translation="MIQRVLAIDGDTMGRGDEALGGRLMVKFIHQLPGVSDRPHAIVF
                     YNTGVRLLADRSVVLDTLRQLEHDGVELLACGTCVAHFGLETRLAAGRVTDMREIAAT
                     LTGAERVVRI"
     gene            391..2679
                     /gene="bglX"
                     /locus_tag="PKK95_13990"
     CDS             391..2679
                     /gene="bglX"
                     /locus_tag="PKK95_13990"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HOC19379.1"
                     /translation="MNRRKLLAAAALAVFALLVPAGPTAAQSLVDPAAEKKIDDLLAR
                     MTIEEKAGQLNQYSSSFDLTGPAPSAGGQKAVYEQIRTGLVGSMLNVIGAEATRKAQQ
                     LAVENSRLRIPLIFGYDVIHGYKTIFPIPLGEAASWDPAAVERSARVAATEAAAAGLH
                     WTFAPMVDIARDARWGRIMEGSGEDPYLGGQMAAARVRGFQGDSLSATDTVAACVKHY
                     AAYGFAEAGRDYNTVDISEQTLRNVVLPPFKAAADAGAATFMNSFNEIAGIPATGSVH
                     LQREILKGEWGFTGFVVSDWGSIGEMLPHGYSKDLEQAAEQAITAGSDMDMESRAYVG
                     HLAGLVKAGRVDERLVDDAVRRVLRVKFALGLFDDPYRYSDTAREKAATLTPANLEAA
                     RDVARRSIVLLKNGGGLLPLRKDLKTIAVIGPFAADKDAPLGNWRGQGEANSAVSLLE
                     GVKAAVSPATEVLHAEGVKLVTGPRNFMAPPVYNTTDRSGIPAAAEAARKAEVVLLAI
                     GEDAFQSGEGRSQADIGLKGLQDELLRAVVAANPKVVVVLSGGRPLAIPDVAEAVPAL
                     VETWLLGSQSGHAIADVLFGDFNPSGRLPVSFPRHVGQMPLYYAHKNTGRPGPQDVVT
                     WSHFTDVPNDALFPFGFGLGYTTFAYSDPVLSASEIGAGGELQVRVRVTNTGSRPGTE
                     VVQLYVRDLVGSVTRPVKELKGFQRVELAAGESREVSFTLTPEDLAFHTARGQWEAEP
                     GAFTVMVGGNSRDVNSASFTLR"
     gene            2690..3556
                     /locus_tag="PKK95_13995"
     CDS             2690..3556
                     /locus_tag="PKK95_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOC19380.1"
                     /translation="MNLSRFQVLMAAILFSTGGAAIKAVTLTPWQVASFRAGIAALTL
                     LLLVPDARRLSWRALAVGAVQAGTFITFVLANRMTTAASAVFLQAAAPFWLILFGPLL
                     LGERSRRSDVPFLGVLFLGVLLLMLGTPGPSATAPDTFRGNVVGAASGFMWALSLAGL
                     RWAGREAARGASFTGASSSVCGNVIACAACLPLALPVGASVPADWLVLVYLGVAQVGL
                     AYVFLTRGLGKVRAMEASLLLLVEPALSPFWAWLAHGEQPGWVAVLGGVIILSATAWR
                     SARPAGQAAGEE"
     gene            3564..>4528
                     /locus_tag="PKK95_14000"
     CDS             3564..>4528
                     /locus_tag="PKK95_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19381.1"
                     /translation="MMPTFIAMVLAAAALQQAPPANYDEAKVPRYTLPPLLVMSDGRP
                     VRDPAAWEVRRREILALLESQMFGRAPRPPADIAFEVLGVDQAALNGIAIRKDVAIGI
                     KGKTLRLQLYLPAGAKRPVPVFLGLGFAPNQTVAADPGGPLAGAWARGDQSKEASLQA
                     AAEASRGAAASRWQVEKLLARGYGLATMYYGDIEPDFDGSMRQGIRAAFVAPGQQAPG
                     DAEWGAIAAWAWGLGRGVDYLVTDKDVDASRIAVVGHSRLGKTALWAGATDPRIAMVI
                     SNDSGEGGAAISRRRFGETVADLNRRFPHWFCGNYRQYSDREDEMP"
BASE COUNT          637 a         1520 c         1685 g          686 t
ORIGIN      
        1 ctagatcctg acgacgcgct cggcgccggt cagcgtggcc gcgatctccc gcatgtcggt
       61 cacgcgaccg gcggccaggc gggtctccag gccgaagtgc gccacgcacg tcccgcaggc
      121 cagcagctcc accccgtcgt gctcgagctg ccgcaaggtg tcgaggacga ccgatcgatc
      181 cgcgaggagc cgcacacccg tgttgtagaa gacgatcgcg tgggggcggt cggacacgcc
      241 aggcagctgg tggatgaact tgaccatcag acggccgccg agcgcctcgt cgccccgccc
      301 catcgtgtcg ccgtcaatcg cgaggacccg ttgtatcatt cgcggatctt atccgacctg
      361 gtggctttcc ctctgccgcg gaggcgcccg atgaaccgac gcaagctttt ggccgctgct
      421 gccctcgccg tgttcgcgct cctggtgccg gctgggccga cggcggcaca gtcgctcgtc
      481 gacccggccg ccgagaagaa gatcgacgac ctcctggcga ggatgacgat cgaggaaaag
      541 gctgggcagt tgaaccagta cagcagctcg ttcgacctca ccggcccggc gccgagcgcg
      601 gggggtcaga aggcggtcta cgagcagatc cgcaccggcc tggtcgggtc gatgctgaac
      661 gtgatcggcg ctgaagccac gcgcaaggcg cagcagctgg cggtcgagaa cagccggctg
      721 aggatcccgt tgatcttcgg gtacgacgtc atccacggct acaagacgat cttcccgatc
      781 ccgctcggcg aggcggcgag ctgggacccc gccgccgtgg agcgttccgc gcgcgtggcg
      841 gccaccgagg cggcggcggc cggccttcac tggacgttcg caccgatggt ggacatcgcc
      901 agggacgccc gctggggtcg catcatggag ggctccggcg aggacccgta cctcgggggg
      961 cagatggcgg cggcgcgcgt caggggcttc cagggagaca gcctgtcggc gaccgacacg
     1021 gtggccgcct gcgtcaagca ctacgccgcc tacggctttg ccgaagcggg ccgcgactac
     1081 aacaccgtgg acatctccga gcagacgctt cgcaacgtcg tgctgccgcc gttcaaggcc
     1141 gcggccgatg ccggcgccgc gacgttcatg aactcgttca acgagatcgc ggggatcccg
     1201 gcgaccggca gcgtgcacct gcagcgagag atcctgaagg gggagtgggg cttcacgggc
     1261 ttcgtggttt ccgactgggg ctcgatcggc gagatgcttc ctcacgggta ctcgaaggac
     1321 ctggagcagg cagccgagca ggcgatcacc gccggcagcg acatggacat ggaatcccgc
     1381 gcgtacgtcg gtcacctcgc cggcctcgtg aaggccgggc gcgtggacga gcggctcgtg
     1441 gacgacgcgg tgcggcgggt gctgcgggtg aagttcgcgc tcgggctgtt cgacgacccg
     1501 taccgatatt cggacacggc gcgggagaag gcggccacgc tgacgccggc caacctcgag
     1561 gcggcccgcg acgtggcgcg ccggtcgatc gtgctgttga agaacggggg cgggctgctg
     1621 ccgctgcgga aggacctgaa gacgatcgcg gtgatcgggc cgttcgccgc cgacaaggat
     1681 gcgccgctcg ggaactggcg cgggcagggg gaggccaact cggccgtctc gctgctcgag
     1741 ggggtgaagg ctgcggtgtc gccagccacc gaggtgctgc acgccgaggg cgtgaagctg
     1801 gtgacggggc cgcgcaactt catggcaccc ccggtgtaca acaccaccga ccgctccggc
     1861 atcccggcgg cagcggaggc ggccaggaag gccgaagtcg tgctgctggc catcggcgag
     1921 gacgccttcc agtcgggcga ggggcggagc caggccgaca tcgggctcaa ggggctgcag
     1981 gacgagctgc tgcgggcggt agtcgccgcg aacccgaagg tggtggtcgt gctgagcggc
     2041 ggccggccgc tggcgatccc ggacgtcgcc gaggccgttc cggcgctggt cgagacctgg
     2101 ctgctcgggt cgcagtcggg ccatgcgatt gccgacgtcc tgttcggcga cttcaacccg
     2161 agcggcaggc tgccggtgtc gttcccgcgg cacgtgggcc agatgccgct gtactacgcg
     2221 cacaagaaca ccggccgccc ggggccgcag gacgtcgtca cctggtcgca cttcaccgac
     2281 gtccccaacg acgcgctgtt cccgttcggg ttcggcctcg gctacacgac gtttgcctac
     2341 tccgacccgg tcctgagcgc ctcggagatc ggcgccggcg gcgaactgca ggtgcgcgtg
     2401 agggtgacga acaccggctc gcgtccaggg acggaagtcg tgcagctgta cgtgcgcgac
     2461 ctggtgggca gcgtgacccg tccggtcaag gagttgaagg ggttccagag ggtggaactc
     2521 gccgcgggtg aatcgcgcga ggtgagtttc acgctgacgc cggaggacct ggcattccac
     2581 acggcccgcg gccaatggga ggccgagccc ggcgcgttca ccgtcatggt gggcggcaac
     2641 tcgcgcgacg tgaattcggc atcgttcacg ctccgctgac gccgttctca tgaacctctc
     2701 gcgattccag gtgctgatgg cggccatcct gttctccacg gggggggccg ccatcaaggc
     2761 cgtcacgctg accccgtggc aggtggcgag cttccgggcg gggatcgccg cgctcacgct
     2821 gctcctgctg gtgcccgacg cacgccggct gtcgtggcgg gcgctggcgg tcggcgccgt
     2881 gcaggccggc acgttcatca ccttcgtgct cgccaaccgg atgaccaccg cggccagcgc
     2941 cgtcttcctc caggcggccg ccccgttctg gctgatcctc ttcgggccgc tgctgctcgg
     3001 agagcgttcg cgccgcagcg acgtgccgtt cctcggggtg ctcttcctgg gtgtcctgct
     3061 gctcatgctc gggacaccgg gaccttcggc caccgccccc gacacgtttc ggggcaacgt
     3121 ggtgggcgcc gccagcgggt tcatgtgggc gctgtcactg gcggggctgc gttgggcggg
     3181 gcgggaggcg gccaggggcg cgagcttcac gggcgcgtcc tcgtcggtgt gcgggaacgt
     3241 gatcgcctgc gccgcgtgcc tgccgctggc gctgcccgtc ggggcctcgg tcccggccga
     3301 ctggttggtg ctggtctacc tgggcgttgc ccaggtcggg ctggcctacg tcttcctaac
     3361 gcgcgggctg gggaaggttc gggcgatgga agcgtcgctg ctgctgctcg tcgagccggc
     3421 cctcagcccc ttctgggcct ggctggcgca cggagagcag cccggctggg tggccgtcct
     3481 gggcggggtt atcatcctga gcgcaaccgc ctggcggtcg gcccggccgg ccgggcaagc
     3541 tgccggagag gagtagcggt tccatgatgc cgaccttcat cgcgatggtt ctggccgccg
     3601 cggcactgca gcaggcgccc cccgccaact acgacgaggc gaaggtgccg cgctacacgc
     3661 tgccgccgct gctcgtgatg agcgacggcc ggcccgtgag ggacccggcc gcctgggagg
     3721 tgcggcgcag ggagatcctc gcgctgctcg agtcgcagat gttcggccgg gcaccgcggc
     3781 cgccagcgga catcgccttc gaggtccttg gggtggacca ggccgcattg aacggcatcg
     3841 ccatccggaa ggacgtcgcg attggaatca aggggaagac cctgcggctt cagctctacc
     3901 tgcccgccgg cgccaagcgg ccggttcccg tcttcctcgg cctgggcttc gctccgaacc
     3961 agaccgtcgc cgccgatccg ggcggaccgc tggcaggagc ctgggcgcgc ggcgaccagt
     4021 cgaaggaggc gtcgctccag gcggcggccg aggcttcgcg cggcgcggcg gcctcgcgct
     4081 ggcaggtgga gaagctgttg gcgcgggggt acggcctggc gaccatgtac tacggcgaca
     4141 tcgagcccga cttcgacggc tccatgaggc agggcatccg tgcggcgttc gtcgcgcccg
     4201 gccagcaggc gcccggcgac gcggaatggg gcgccatcgc ggcctgggcg tggggcctcg
     4261 gccgcggcgt cgattacctg gtcaccgaca aggacgtcga tgcgtcgcgc attgccgtgg
     4321 tcggccattc gcggctcggc aagacggcgc tctgggctgg cgcgacggac ccgcgcatcg
     4381 cgatggtgat ctcgaacgac tcgggcgaag ggggcgcggc catcagccgc cgccggttcg
     4441 gggagacggt tgcggatctg aaccggcgct tcccgcactg gttctgcggc aactatcgcc
     4501 agtacagcga ccgcgaggac gagatgcc
//
LOCUS       DAOPQX010000312         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_20997_length_4494_cov_4.275287, whole genome shotgun sequence.
ACCESSION   DAOPQX010000312 DAOPQX010000000
VERSION     DAOPQX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20997_length_4494_cov_4.275287"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PKK95_14005"
     CDS             complement(<1..1316)
                     /locus_tag="PKK95_14005"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOC19382.1"
                     /translation="MPRPLRTAPFLALFVLLAPGLLPGGPGGALALAQESPAAPAATP
                     DNPFGRKYPPRILNATRLQGKPPSIDGRFDDEAWNEGTWEGDFKQQLPIEGAPPSQPT
                     EVKILYDDGYVYMAIRAYDDPAKVHRYPGRRDDFNGYAVDVVGVCFDSYNSKRTGFEF
                     DLTAGGGKIDLVLGNGEQEWDTTWDAVWDGKVAHDDKGWTAEFRVPLNQLRYGPQEEQ
                     VWGLHVWRWIARNGEEDQWQLIPRQNTGRMYQLGELRGIRGLPRSRHVELMPHLLGKT
                     SSGPSVPGGGTDAAGASGLDAKVGLTSNFTLDGTVNPDFGQVEADPSVINLTAYETFY
                     QEKRPFFLEGRKILEFQLEQEEQLFHSRRVGGAPAYSPPLGAGEEAHLPESTTILGAF
                     KVTGRTDDGLSVAVLQGFTERETVHVSSPTRPRTAVVQPFGSYSVAR"
     gene            complement(1369..2721)
                     /locus_tag="PKK95_14010"
     CDS             complement(1369..2721)
                     /locus_tag="PKK95_14010"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC19383.1"
                     /translation="MASGVSRRTFLKAASAFPAIVPASVLGRHAPNGQVAIGAIGTGR
                     ISRVHDLPGVWKHPAARVVAVCDVDSKRLADARRLVNGHYAEKTGKPYDGVRAYADYR
                     ELLANEGIDAVLVSTPDHWHALITVAAVRAGKDVYLQKPASLTIAEGRFVAREVARSR
                     RIVQVGSQQRSSPQFRYAAELVRNGRIGKLHTVKIGLPGDPPGRDEPEMPVPPNLDYD
                     TWLGSTPFAYYTENRVHPQQGYGRPGWLRCEQFGAGMITGWGSHHLDSAHWAMDEEHS
                     GPLEIWGSAEFPAGGLWDVHGPFKTEAVYATGAHMIVSGDFPNGIRFEGSEGWIFVSR
                     GNEAVTASDPQSALKDAQALAASDPAIITSVIGPNEVHLEESTDHHGNWIDAVRSRRQ
                     PIAPFDVAHRSCSACLLHHIAMKTGRRMRWDPAAERFIGDDEANAMLSRPQREGYRLA
                     "
     gene            complement(2733..3890)
                     /locus_tag="PKK95_14015"
     CDS             complement(2733..3890)
                     /locus_tag="PKK95_14015"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC19384.1"
                     /translation="MRHDGIDRRGFVVGALGSAAAVAGLRASGVPGVDGMTGPVRTER
                     VRRGSPQPPKPRIRFAVIGMDHPHIYSQVAAVERGGGELVFVHAKEPQQLAEFTKRVP
                     RGKAARSEAEILDDPSIQLVVSSIVPDERAPLGIRVMQHGKDYMADKPGITTLEQLAE
                     VRRVQAETKRIYSIMYSERLENRATVRAGELVAAGAIGRVVQTIGLGPHRISLNTRPA
                     WFWDKARFGGILCDIASHQADQFLFFTNSTSAEVIAAQVANVHHPQYPKFEDFGDMLL
                     RGNGGRGYIRVDWFTPDGLATWGDGRLTVLGTDGFIEIRKNIDIAGRPGDSHLFLVDH
                     KETRYIDCREQPLPYGERLVDDVLNRTETAMPQAHCLLAAQLVLEAQKKAV"
     gene            complement(3958..>4494)
                     /locus_tag="PKK95_14020"
     CDS             complement(3958..>4494)
                     /locus_tag="PKK95_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19385.1"
                     /translation="TVHAWLFDLLYPWATSDWPVERNAFFLRLALYLVFGGLLVAVNV
                     VFDYAKIRAVVEDRRSALGALIAAVRFIRRHPGPVAGLYALNGALFLLILALYALAAP
                     GARGGWFVWLGFLVTELYLLARLALKLTFYASETSLFQRLLAHAEYTASPPPAWPESP
                     AAEAIAPPSSPSGGGIES"
BASE COUNT          644 a         1587 c         1575 g          688 t
ORIGIN      
        1 cgcgccaccg agtagctgcc gaacggctgg acaaccgccg tgcgagggcg tgtcggcgac
       61 gaaacgtgca cggtctccct ctcggtgaac ccctgcaaga cggcgaccga caggccgtca
      121 tccgtgcgcc ccgtgacctt gaacgcgccg aggatcgtcg tgctctcggg aaggtgggcc
      181 tcctcgcccg cgccgagcgg cggcgagtag gccggggcgc cgccgacgcg ccgcgagtgg
      241 aagagctgct cctcctgttc gagctggaac tcgaggatct tccgcccctc gaggaagaac
      301 ggccgcttct cctggtagaa ggtctcgtag gcggtcaggt tgatgaccga cgggtcggcc
      361 tccacctggc cgaaatcggg gttgaccgtg ccgtcgagcg tgaagttcga cgtgaggccg
      421 accttcgcgt cgaggccgga cgccccggcc gcgtcggtgc cgccgcccgg aacggacggc
      481 cccgagctgg tcttgccgag caggtgcggc atcagctcca cgtggcgcga acgcggcaac
      541 ccccgaatcc cgcgcagttc gccgagctgg tacatccgcc ccgtgttctg gcgcgggatc
      601 agctgccact ggtcctcctc gccgttgcgg gcgatccacc tccagacgtg gagcccccac
      661 acctgctcct cctgcgggcc gtaacgcagc tggttcagcg gcacccggaa ctcggcggtc
      721 catcccttgt cgtcgtgggc gaccttgccg tcccagaccg catcccacgt ggtatcccac
      781 tcttgctcgc cgtttccgag gaccaggtcg atcttccccc cgccggccgt caaatcgaac
      841 tcgaacccgg ttcgcttgct gttgtagctg tcgaagcaga cgccgacgac gtccaccgcg
      901 tagccgttga agtcgtcgcg ccggcccggg taccggtgca ccttggccgg gtcgtcgtag
      961 gcccggatcg ccatgtagac gtagccgtcg tcatagagga tcttcacctc cgtcggctgg
     1021 gagggcggcg ccccctcgat cggcagctgc tgcttgaagt cgccctccca agtcccctcg
     1081 ttccaggcct cgtcgtcgaa gcggccgtcg atggacggcg gttttccctg caggcgcgtc
     1141 gcgttgagga tgcgcggcgg gtacttgcgg ccgaacgggt tgtcgggggt ggccgccggt
     1201 gcggcagggc tctcctgcgc gagcgcgagg gcgccgcccg ggccgccggg gagcagcccg
     1261 ggcgcgagaa ggacgaacag ggccaggaac ggggcggtgc ggagcgggcg cggcatgtcc
     1321 cgcgaaggct atcttccgcg ccgcccgcag tcaatgacgg gcacagcgtc aggcgagccg
     1381 gtatccctcc cgctgcggcc tcgacagcat cgcgttcgcc tcgtcgtcgc cgatgaaccg
     1441 ctcggcagcg gggtcccagc gcattcgccg cccggtcttc atcgcgatgt ggtgcagcag
     1501 gcacgccgag caggagcggt gggcgacgtc gaacggcgcg atcggctgcc gacgcgagcg
     1561 taccgcgtcg atccagttcc cgtggtggtc ggtgctctcc tcgaggtgca cctcgttcgg
     1621 cccgatcacc gaggtgatga tcgcggggtc gctggcagca agcgcctgcg cgtccttcag
     1681 cgccgactgc gggtcgctcg cggtgaccgc ctcgttgccg cgcgacacga agatccaccc
     1741 ctcgctgccc tcgaaccgga tcccgttggg gaagtcgccg ctgacgatca tgtgcgcgcc
     1801 ggtcgcgtag accgcctcgg tcttgaacgg cccgtgcacg tcccacagcc cgcccgccgg
     1861 gaactcggcg ctgccccaga tctccagcgg gccgctgtgc tcctcgtcca tcgcccagtg
     1921 cgcgctgtcg aggtggtgcg agccccagcc ggtgatcatc cccgcgccga actgctcgca
     1981 gcgcagccaa ccgggacggc cgtagccctg ctgcgggtgc acccggttct ccgtgtagta
     2041 ggcgaacggg gtcgagccga gccaggtgtc gtagtcgagg ttcggcggga ccggcatctc
     2101 gggctcgtcc cgtcccggcg ggtcgccggg cagaccgatc ttcaccgtgt gcagcttccc
     2161 gatgcggccg ttccgcacca gctcggcggc gtagcggaac tggggagacg agcgctgttg
     2221 gctgcccacc tgcacgatcc gccggctgcg cgccacctcg cgggcgacga agcggccctc
     2281 ggcgatggtg agcgaggccg gcttctgcag gtagacgtcc ttgccggccc gcaccgccgc
     2341 aacggtgatc agcgcgtgcc agtggtcggg ggtgctcacc agcacggcgt cgatgccctc
     2401 gttcgccagc agctcgcggt agtcggcgta ggccctgacg ccgtcgtacg gcttgccggt
     2461 cttctcagcg tagtgcccgt tgaccaggcg cctggcgtcc gccaggcgct tcgagtcgac
     2521 gtcgcacacc gccaccaccc gggcggcagg gtgcttccag acgccgggca ggtcgtgcac
     2581 gcgcgagatg cggccggtgc cgatggcgcc gatggccacc tggccgttcg gcgcgtggcg
     2641 gccgagcacc gacgcgggga cgatggcggg gaaggcggag gccgccttga ggaacgtgcg
     2701 acgcgatacg cccgatgcca tgagcacccc ctctacacgg ccttcttctg cgcctcgagc
     2761 accagctgcg ccgccagcag gcagtgagcc tgcggcatgg cggtctcggt gcggttcagc
     2821 acgtcgtcca ccaggcgctc gccgtacggc agcggctgct cgcggcagtc gatgtagcgg
     2881 gtctccttgt ggtccaccag gaacaggtgg ctgtcgccgg gccgccccgc gatgtcgatg
     2941 ttcttgcgaa tctcgatgaa gccgtcggtg ccgaggacgg tcagccgtcc gtcgccccac
     3001 gtggcgaggc cgtcgggggt gaaccagtcc acccggatgt agccgcggcc gccgttgccc
     3061 cgcaggagca tgtcgccgaa gtcctcgaac ttcgggtact gggggtggtg gacgttggcc
     3121 acctgcgcgg cgatcacctc ggcggaggtg gagttggtga agaacaggaa ctggtcggcc
     3181 tggtgcgagg cgatgtcgca caggatgccc ccgaatcgcg ccttgtccca gaaccacgcg
     3241 ggacgcgtgt tgaggctgat gcggtgcggc ccgagcccga tcgtctggac gacccggcca
     3301 atcgcgccgg cagccaccag ctcgcccgcc cgcacggtgg cgcggttctc gagccgctcg
     3361 ctgtacatga tcgagtagat gcgcttcgtc tcggcctgca cccggcgcac ctcggccagc
     3421 tgctccagcg tggtgatgcc cggcttgtcg gccatgtagt ccttgccgtg ctgcatgacg
     3481 cggatgccga ggggcgcgcg ttcgtcgggg acgatcgagc tcacgaccag ctggatcgac
     3541 ggatcgtcga ggatctcagc ctcgctgcgc gcggccttgc ccctgggaac gcgcttggtg
     3601 aactccgcca gctgctgtgg ctctttcgcg tgaacgaaca ccagctcccc gcctccccgc
     3661 tccaccgccg ccacctggct gtagatgtgc gggtggtcca tgccgatcac ggcgaagcgg
     3721 atgcgcggct tggggggctg cggggaacct cggcgcaccc gctcggttcg caccgggccc
     3781 gtcatcccgt ccaccccagg cacccctgac gcgcgcagcc ccgcgacggc cgccgccgag
     3841 ccgagcgcgc ccaccacgaa gcctcgacga tcgattccgt cgtgtcgcat gtgtgcagcc
     3901 tccatggggg cagatccagg gggagtgtgc cggggcagag gtgacagaac agccagctca
     3961 ggattcgatt ccaccgcccg aaggcgagga tggcggcgcg atcgcctctg ccgccggcga
     4021 ttcgggccag gcgggcggcg gcgacgccgt gtactccgcg tgcgcgagca ggcgctggaa
     4081 cagcgaggtc tccgacgcgt agaacgtgag tttcaaggca aggcgcgcca gcaggtacag
     4141 ctcggtgacg agaaagccga gccacacgaa ccacccgcct cgcgcgccgg gtgccgccag
     4201 cgcgtagagc gccaggatca gcaggaacaa ggcgccgttc agcgcgtaga gcccggcgac
     4261 cggcccgggg tgccggcgga tgaaccgcac ggccgcgatc agcgccccga gagcgcttcg
     4321 ccgatcctcg accaccgccc tgatcttcgc gtagtcgaac accacgttca ccgcgaccag
     4381 gagcccgccg aacacgaggt acagggccag ccgcaggaag aaggcgttcc gctcgaccgg
     4441 ccagtcgctg gtcgcccacg ggtagagcag gtcgaacagc caggcgtgga ccgt
//
LOCUS       DAOPQX010000313         4468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21160_length_4468_cov_4.780195, whole genome shotgun sequence.
ACCESSION   DAOPQX010000313 DAOPQX010000000
VERSION     DAOPQX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21160_length_4468_cov_4.780195"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKK95_14025"
     CDS             complement(<1..718)
                     /locus_tag="PKK95_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOC19386.1"
                     /translation="MRRGLALVLVLIVAAVAVSLAGLAVLWAIVGRGPAVPSSASLVL
                     RLTGDPVEGGGEEGISQFIPGDREPSVRSIVENIRKAKADRRVRALVVAPINLGSPYA
                     AKLQEIRDAIRDFRREGKPAIGYLEDGSQDAYYLATACDRIYLMPSSPLQLTGEASYE
                     LFLRGTLDKIGAYPDMLHVGQYKTAVNQLTEKTFTPAHREMAESLNRDRYEQLVTAIA
                     DARGKSEDEVRALIDEGPYLP"
     gene            complement(773..1894)
                     /locus_tag="PKK95_14030"
     CDS             complement(773..1894)
                     /locus_tag="PKK95_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC19387.1"
                     /translation="MRIPNFEMERMQSTWENLVAFDMSESGVRPVTLRELTALGLDLE
                     AALDMPLGYSQSNGTIALRERLAAIYPGATVDHIEVTNGTSEANYLVALALLQPGDEM
                     AMEIPNYMQLWGVPQSLQAPLRTFRLRPEAAWEPDWEEFERAVTPKTRLLYLSNPNNP
                     SGAVLSASAMSRIVRRCEETGTILLADEVYLGAELEGERTKSFWCMSPQVVVTSGLSK
                     AYGIPGVRIGWIVGPPPLIETCWSQHDYLTIGPNKLSDLVARTAVTVENREHLYARTR
                     SILRANLPLIRDWAAGFPGLLELNEPKAGAMALVRYHAEAPSLEVCERIRVEQSTLVV
                     PGTHVGLEGYLRIWYGGRPEYISEGLRRIGMVLGSLAPA"
     gene            1915..2919
                     /locus_tag="PKK95_14035"
     CDS             1915..2919
                     /locus_tag="PKK95_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOC19388.1"
                     /translation="MNRPARDVTVLAEPDIRRLVPLDLEALAAVEHAFARLAEGRADV
                     PPIVGLFVPERQGEVDVKGAYIHGLPRMAIKIASGFYGNPARGLPSGSGMMVVVSAET
                     GFPEAVLLDNGYLTEIRTGLAGAAAARYLAPSTVSTVGVIGAGSQGRYQVRALRLVRS
                     FERVLVFDRTRESAEAYAREMHPELGVRVEPASAEAVVRESQVVISCTPSREPIVKAA
                     WLHAGMHVTAMGADTAEKQELESAVLARADRLACDYRRQCFERGEFLHALRDAAIGAG
                     ADVTELGELTSGRRQGRQQPGEITVCALTGVGVQDAAIALLCYERAVAAGAGLRVGAQ
                     "
     gene            complement(2941..>4468)
                     /locus_tag="PKK95_14040"
     CDS             complement(2941..>4468)
                     /locus_tag="PKK95_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC19389.1"
                     /translation="VDARLLDRDARRVEVNPSARRLLGLPTATAAILEDVPGHVRLVK
                     DGRDLPFEQLPLREAARGGHLADYEVDVVREDGEVRTLFGNATSLRDENGEICGAVGA
                     FVDVSERKRAEEALRHADRIKDDFLATLSHELRTPLNAIVGWASMLVEGRLAPEAVPR
                     AYDSIARNAAAQRHLIEDVLDVSRIISGKLQLDSSHLDAGVPLRAAIDAVRPAAEARG
                     IAIAVSEPPGPLFVNGDGARLQQVFWNLLVNAVKFSADGGRVEVGLRAADESVEVQVR
                     DHGIGISQEFLPHVFDRFRQLDSSATRKYGGLGLGLSIVQHLVELHGGTVTASSEGEG
                     TGTTMVVRLPASPLGDRRRGDASRPARVPAVSDPVDAQPLLGLRVLVVDDQPDAREMA
                     ALVLGNHGAEVVTAASAAEALDQLSKGEPHVLVIDLAMPQVDGHSLIRTIRAAGGRLA
                     ETPAIALSAYARNEDRQRALAAGFQLHLAKPAEPRVLVRAVAGLAADSRAPRGAVPKA
                     "
BASE COUNT          621 a         1606 c         1575 g          666 t
ORIGIN      
        1 cgggcaggta cggcccctcg tcgatcaggg cccgcacctc gtcttcgctc ttcccgcgcg
       61 cgtcggcgat cgccgtgacc agctgttcgt accggtcgcg gttcagcgac tcggccatct
      121 cgcggtgcgc cggggtgaac gtcttctccg tgagctggtt gaccgccgtc ttgtactgcc
      181 cgacgtgcag catgtcgggg taggcgccga tcttgtcgag cgtgcctcgc aggaacagct
      241 cgtagctggc ctccccggtc agctgcaggg ggctcgaggg catcaggtag atgcggtcgc
      301 acgccgtcgc caggtagtag gcgtcttgcg agccgtcctc gaggtacccg atcgccggct
      361 tcccctcgcg gcggaagtcg cggatcgcgt cgcggatctc ctggagcttc gcggcgtagg
      421 gcgatccaag gttgatcggc gccacgacca gggcccgcac ccgccggtcg gccttggcct
      481 tgcggatgtt ctcgacgatc gaccgcacgc tcggctcgcg gtcgccgggg atgaactgcg
      541 agatcccctc ctcgccgccc ccctccaccg ggtcgccggt gagccgcagg accagcgacg
      601 ccgaggacgg caccgccggc ccccgcccga cgatggccca caggacggcc aggccggcca
      661 gcgacacggc caccgccgcg acgatcagca ccagcacgag agcaagtccg cgacgcacgg
      721 cctacctcct gaaaggagac acgagtatag cagcgcaacg gcagaggtct cgttacgcgg
      781 gtgccagcga ccccagcacc atcccgatgc gccgcagccc ttcgctgatg tactccggcc
      841 ggccgccgta ccagatccgc aggtacccct ccagcccgac gtgagtcccc ggcacgacga
      901 gggtgctctg ttcgacccgg atccgctcgc acacctcgag gctgggcgcc tcggcgtgat
      961 agcgcacgag cgccatcgcc cccgccttcg gctcgttcag ctcgagcagg ccgggaaaac
     1021 ccgccgccca gtcgcggatc agcgggaggt tcgcccgcag gatggaacgg gttcgcgcgt
     1081 agaggtgctc gcggttttcc acggtcaccg cggttcgcgc gacgagatcc gagagcttgt
     1141 tcgggccgat ggtcaggtaa tcgtgctggc tccagcaggt ctcgatgagc gggggcgggc
     1201 cgacgatcca tccaatgcga accccgggga tgccgtacgc cttcgacagc ccgctggtca
     1261 cgacaacctg cgggctcatg caccagaagc tcttcgtccg ctccccttcc agctcggcgc
     1321 ccaggtacac ctcgtcggcg agaaggatgg tgccggtctc ctcgcagcgc cggacgatgc
     1381 gcgacatcgc cgaagcggag aggacggcgc ccgaggggtt gttcgggttg gagaggtaca
     1441 gcagccgcgt cttcggcgtg acggctcgct cgaactcctc ccaatcgggc tcccaggcgg
     1501 cctcgggccg cagccggaac gtgcgcagcg gagcctgcag gctctgcggc acgccccaca
     1561 gctgcatgta gttcgggatc tccatcgcca tctcgtcgcc gggctgcagg agcgcgaggg
     1621 cgacgaggta gttcgcttcc gaggtcccgt tggtcacctc gatgtgatcg acggtggccc
     1681 ctggatagat cgccgccagg cgctcgcgga gcgcgatcgt gccgttcgac tggctgtagc
     1741 cgagcggcat gtcgagcgcc gcctcgaggt cgagcccgag cgccgtcagc tcgcgaagcg
     1801 tcaccggcct gacgccgctc tcgctcatgt cgaacgccac caggttctcc caggtggact
     1861 gcatccgctc catctcgaag ttcggaatgc gcatacgctg tgtattctat gccgatgaac
     1921 cgacctgccc gggacgtgac cgtcctggcc gagcccgaca tcaggcggct cgtcccgctc
     1981 gacctggagg cgctggccgc cgtcgagcat gccttcgcgc gcctggccga ggggcgggcc
     2041 gacgtgccgc cgatcgtggg ccttttcgtc cccgagcgcc agggcgaggt tgacgtgaag
     2101 ggggcctaca tccacggcct gccgcgcatg gcgatcaaga tcgcgtccgg cttctacggc
     2161 aacccggcgc gcggcctccc gagcgggagc gggatgatgg tggtggtcag cgccgagacg
     2221 ggcttccccg aagcggtgct gctcgacaac ggctacctga ccgagatccg cacagggctg
     2281 gccggggccg cggccgccag gtatctcgcc ccttcgaccg tttccaccgt gggggtgatc
     2341 ggcgccggaa gccagggccg ctaccaggtg cgggcgctgc gcctggtgcg ctccttcgag
     2401 cgggtgctcg tcttcgatcg gacccgcgaa tccgccgagg cctacgcgcg cgagatgcac
     2461 cccgagcttg gcgtccgggt ggagccggcc agcgccgagg cggtcgtgcg cgagagccag
     2521 gtcgtgatct cgtgcacgcc ctcgcgtgag ccgatcgtca aggcggcgtg gctgcacgcc
     2581 ggcatgcacg tgaccgcgat gggagccgac accgccgaaa agcaggagct ggaaagcgcc
     2641 gtgctcgcgc gggccgatcg gctggcgtgc gactacaggc gccagtgctt cgagcgcggc
     2701 gagttcctgc acgcgctgcg ggacgcggcg atcggcgccg gtgccgatgt gaccgagctc
     2761 ggcgagctga cgtcgggccg ccggcaggga cggcagcagc ccggcgagat cacggtgtgt
     2821 gcgctgacag gcgtgggcgt gcaggacgcg gcaatcgccc tgctctgcta cgagcgcgcg
     2881 gtcgccgccg gcgccggcct gcgcgtgggc gcgcagtagc acaggcgcgc cggccgttct
     2941 tcacgctttc ggtacggccc cacgtggcgc ccggctgtcg gctgccagcc ccgccacggc
     3001 gcgcacgagg actcgaggct ctgccggttt ggccaggtgc agctggaacc ctgccgccag
     3061 ggcccgttgc cggtcttcgt tcctggcgta cgcgctcagg gcgatggccg gcgtctcggc
     3121 aagccggccg cctgccgcgc ggatcgtccg gatcagcgaa tgcccgtcca cctgcggcat
     3181 cgccaggtcg atgacgagca cgtgcggctc gcccttcgac agttggtcga gcgcctcggc
     3241 tgccgacgcc gccgtgacga cctccgcgcc gtggttgccg aggaccaggg ccgccatctc
     3301 ccgggcgtcc ggctggtcgt ccaccaccag cacgcgcagc ccaaggagcg gctgggcgtc
     3361 caccgggtct gaaaccgccg ggacgcgtgc cggcctggac gcgtccccgc ggcgccggtc
     3421 gccgagcggc gacgccggca gtctcacgac catcgtggtc ccggtgccct ccccctcgct
     3481 gctggcggtc accgtcccac cgtgaagctc cacgaggtgc tggacgatcg acaatcccag
     3541 cccgagccct ccgtacttcc gggtcgccga gctgtccagc tgccggaagc gatcgaagac
     3601 gtgcggcagg aactcctggc tgatgccgat cccgtggtcc ctcacctgga cttcgacgct
     3661 ctcgtcggcg gcccggaggc cgacctccac ccggccgccg tcggcgctga atttcaccgc
     3721 gttgacgagc aggttccaga acacctgctg caggcgcgcg ccgtcgccgt tcacgaagag
     3781 cggccccggg ggctcggaga cggcgatggc gatgcctcgc gcctcggcgg ccggccgcac
     3841 ggcgtcgatg gcggcccgaa gcggcacgcc ggcgtccagg tggcttgaat cgagctgcag
     3901 cttgccgctg atgatccggg agacgtcgag gacgtcctcg atgaggtggc gttgggcggc
     3961 ggcgttgcgc gcgatcgagt cgtaggcccg gggaacggcc tccggcgcca ggcgcccctc
     4021 gaccagcatg ctggcccacc ccacgatggc gttgaggggc gtccgcagct cgtgcgacag
     4081 cgtcgcgagg aagtcgtcct tgatccgatc cgcgtgacgc agggcctcct cggcgcgctt
     4141 gcgctccgag acgtccacga acgccccgac ggccccgcag atctcgccgt tctcgtcacg
     4201 gagggaggtg gcattgccga acagggtccg cacctcgccg tcctcgcgca ccacgtcgac
     4261 ctcgtagtcg gcgaggtggc cgccccgcgc tgcctcccgg agcgggagct gctcgaacgg
     4321 caggtcgcgg ccgtccttga cgagccggac gtgccccggg acatcctcca ggatggccgc
     4381 cgtggccgtg ggcagcccga gcaggcggcg tgcggaaggg ttcacctcga cgcggcgcgc
     4441 gtcgcggtcg agaaggcgcg cgtcgacc
//
LOCUS       DAOPQX010000314         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21168_length_4467_cov_6.009293, whole genome shotgun sequence.
ACCESSION   DAOPQX010000314 DAOPQX010000000
VERSION     DAOPQX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21168_length_4467_cov_6.009293"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKK95_14045"
     CDS             complement(<1..685)
                     /locus_tag="PKK95_14045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOC19390.1"
                     /translation="MSADGDRLDELAGAILDGTPIDWDAADEAAVPADRPFVRQLKAI
                     AAIAEVQRGEELGTWGPLRLLERIGQGASGEVYRAWDPRLDREVALKLMQAREAASEE
                     RASSIIEEGRMLARVRHPNVVTIYGAERIGDRIGIWMELVAGRTLHQLVVEEGRRFSP
                     REVAAIGETLCHALSAVHAAGLLHRDVKAQNVMMGADGRVMLMDFGAGGEQSRPSETG
                     LAGTPLYLAP"
     gene            complement(675..1286)
                     /locus_tag="PKK95_14050"
     CDS             complement(675..1286)
                     /locus_tag="PKK95_14050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOC19391.1"
                     /translation="MNDETPPKPDGPGSSAAAVETTVSLLARAREGDPAAVERLFARH
                     LKPLQRFARGRLPGWARDLADTDDLVQDALLQTFKKMEGFESRHPGALQAYLRQAVLN
                     RLRDELRRRARTPARVDIEAAERESAGSLVEEALGPETMAAYEQALGRLRPEEREAVI
                     GRLEMGFSYEELALALEKPTPDAARKAAQRALVRLAEEMHRER"
     assembly_gap    1375..1474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1604..2959
                     /locus_tag="PKK95_14055"
     CDS             1604..2959
                     /locus_tag="PKK95_14055"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HOC19392.1"
                     /translation="MSRLHHTIGIHPLYVAAALVIALAPASPAHAQTPEMLSLKPCEV
                     TPTNDVVGDWSAYMLCAISQDATFRNNSLHGGRYMAMVAAAVFDAVNGVERRYLPIRV
                     EPAAPRGASRRAAAVEAAYRMLIAIFPAQQAELVKKRDASLARITAAAAAGSYEDGDP
                     TLSVSIARGVAWGARVAAEILAWRSGDLPPVPPFLGETAIGKWRPTPPAFASGAGPQF
                     ATMSPWVILAPNQFRPAPPPALTSAQYTADFNEVKASPTPGQVEVARFWNDNTPAHWN
                     RIMREAAAARQLPPSESARLFALLNLSMADAAIVCWEAKYYYNFWRPLTAVELAELDG
                     NPDTAGLTPGTTWTTVLTTPAHPEYPSGHSTQSSAAAEVLASFFGDATSFTTDSEALP
                     VLRNYSSFAAAVDEIHGARIWAGIHFRTACAIGSEIGRAVATYVAAHAMTPVHGDYAG
                     Q"
     gene            3026..>4467
                     /locus_tag="PKK95_14060"
     CDS             3026..>4467
                     /locus_tag="PKK95_14060"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC19393.1"
                     /translation="MLPGISASSREPGPWTALAGRLSLALLVTLVAVGSPVWAQSLPA
                     GWNDDDIGAVGLAGSASYSSGVFTVRGAGADIWGAADSFNFANQILSGDGQVVVRVAS
                     MGNTHTYAKAGVMFRESLTAGSRHVVLDMKPGGGVEFMARSVTGGSTSYIGGGAGRWL
                     KLVRRGSTFTAYVSADGAAWTSIGSRSVTMAANAWVGLVVCSHNTGVLNTATFDRVTV
                     GDGDLPPAVAITSPAAGAAFSAGADVTVTANATDTDATPVAEVEFYANGSLIGRDTSS
                     PYSVTWADVPAGSYSLTAVATDTANLSATSRAVAVTVSGPTGALPTGWADTDIGTVGL
                     AGAASHNAGTFTVRGAGADIWGTADSFHFANRALSGDGQITARVASMDSTHAYAKAGV
                     MIRETLTAGSRHVLLDMKPSGGVEFMARAATGGSTSYIAGGTGRWLRLVRSGSTFTAY
                     VSADGATWRSIGSRSVTMNATVWVGLAVCS"
BASE COUNT          645 a         1563 c         1507 g          652 t
ORIGIN      
        1 cgggcgccag gtagagcggc gtgccggcca agccggtctc ggagggacgg ctctgttcgc
       61 cgcccgcccc gaagtccatc agcatcacgc ggccgtcggc gcccatcatg acgttctgcg
      121 ccttgacgtc gcggtgcagc agtcctgccg cgtgcacggc cgagagcgcg tggcagaggg
      181 tctcgccgat ggccgccacc tcgcgcggcg agaagcgccg cccctcctct acgaccaact
      241 ggtggagcgt gcgcccggcc accagctcca tccagatgcc gatgcgatcg ccgatccgtt
      301 cggccccgta gatcgtgacg acgttagggt gccggacgcg ggcgagcatc cggccttcct
      361 cgatgatgga gctggcccgc tcttcggacg ccgcctcgcg ggcctgcatc agcttcagcg
      421 ccacttcgcg gtcgaggcgt ggatcccagg cccgatagac ctcgccggac gctccttgcc
      481 cgatccgctc cagcagccgc agaggccccc acgtcccaag ttcttcgccg cgctgcacct
      541 cggcgatcgc ggcgatcgcc ttcagctgcc ggacgaacgg gcggtcggcc ggcacggccg
      601 cctcgtcggc cgcgtcccag tcgatggggg tgccgtcgag gatcgccccc gccagctcgt
      661 ccagccggtc gccgtcagcg ctcacggtgc atctcctccg cgagccgaac gagcgcgcgc
      721 tgcgcggcct tgcgcgccgc gtccggcgtc ggcttctcca gcgccaacgc cagttcctcg
      781 tagctgaagc ccatctcgag gcgcccgatc accgcctccc gctcctccgg gcgcaaccgg
      841 ccgagcgcct gctcgtaggc cgccatcgtc tcgggtccaa gggcctcctc gacgagggag
      901 ccagccgact cgcgctcggc ggcctcgatg tcgacccggg cgggcgtgcg ggcgcgccgc
      961 cgcagctcgt ccctcaagcg attgagcacg gcctggcgca ggtacgcctg cagggcgccg
     1021 gggtgacgcg actcgaaccc ctccatcttc ttgaacgtct ggagcagggc gtcctggaca
     1081 aggtcgtcgg tgtcggcgag gtcgcgtgcc cagcccggca ggcggccgcg ggcgaaccgc
     1141 tgcagcggct tgaggtgacg cgcgaagagc cgttcgacgg cggcagggtc gccttcgcgc
     1201 gcacgagcga gaaggctcac ggtcgtttcg acggcagcgg ccgaagaacc cgggccgtcg
     1261 ggtttcgggg gcgtctcgtc gttcatgacg ggttcttcga tggtagtccg attcctgccc
     1321 gcgccgacgt tatccgagtg ggagagggca gccagcccgg ctgttggccc cagtnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcaca ggcgtcccgc ctgtgaagca
     1501 gtagcacagg cgtctcgcct gtgaagcagt agcacaggcg tcccgcctgt gaagcagtag
     1561 cacaggcgtc tcgcctgtga agcagagaaa tggaggaacc cgaatgtctc gccttcacca
     1621 cacgatcggc attcatccgc tgtacgtggc ggccgcgctc gtcatcgcgc tcgcgccggc
     1681 ctcgcctgcg catgcacaga cgccggagat gctgagcctg aaaccctgcg aggtgacgcc
     1741 gaccaacgac gtcgtcggcg actggagcgc gtacatgctg tgcgcgatca gccaggacgc
     1801 caccttcagg aacaactcgc tgcacggcgg ccgttacatg gcgatggtcg ccgccgccgt
     1861 cttcgacgcg gtgaacggcg tcgagcggcg ctacctcccc attcgcgtcg aaccggctgc
     1921 gccccgcggc gcgtccaggc gcgcagccgc cgtcgaggcg gcgtaccgca tgttgatcgc
     1981 gatcttcccc gcacagcagg ccgagctggt caagaagcgc gacgcgtcgc tcgccaggat
     2041 cacggcggcg gccgcggcgg gttcgtacga ggacggcgac cccaccctga gcgtgtcgat
     2101 cgcccgcggc gtcgcgtggg gcgccagggt ggcggcagag atcctggcat ggcgctcggg
     2161 tgatctgccc ccggttccgc cgtttcttgg cgaaactgcg attggcaagt ggcggccgac
     2221 gccgcccgcc ttcgcctcag gggcaggccc gcagttcgcc accatgtcgc cgtgggtgat
     2281 cctcgcgccg aaccagttca ggcccgcgcc gcctcccgcg ctcaccagcg cgcagtacac
     2341 ggccgacttc aacgaggtca aggcgtcgcc gacgcccggg caggtcgagg tggcccggtt
     2401 ctggaacgac aacaccccgg cgcactggaa ccggatcatg cgcgaggcgg cggccgcccg
     2461 ccagctgccc ccgtcagaga gcgcccgcct gttcgccctg ctgaacctct cgatggcgga
     2521 cgcggcgatc gtgtgctggg aggcgaagta ctactacaac ttctggcgcc cgctgacggc
     2581 cgtggagctg gcggaactcg atggcaaccc agacacggcg gggctcacgc ccggcaccac
     2641 gtggaccacg gtactcacca cgccagcgca ccccgagtac ccctccgggc actccaccca
     2701 gagcagcgcg gctgccgagg tgctggccag cttcttcggc gatgccacct cgttcacgac
     2761 cgattcggag gcgttgccgg tgctgcgcaa ttacagcagc ttcgcggcgg ccgtcgatga
     2821 gattcacggc gccaggatct gggcgggcat tcacttccgc acggcctgcg ccatcggcag
     2881 cgaaatcggc agggcggtag ccacctacgt cgcggcgcac gcgatgaccc ccgtccacgg
     2941 cgattacgcg gggcagtagg gcggcaccgc ggttcgacga gggcggctcg accataaccc
     3001 cagcgtcacc ccacgtggag gcagcatgtt gcccggaatc agcgcgtcga gccgcgaacc
     3061 aggtccttgg acagccctcg ccgggcggtt gtcgctggcg ttgttggtca cgctcgtggc
     3121 agtgggcagc cccgtttggg cccagtcgct tcccgccggc tggaacgacg acgacatcgg
     3181 agcggtcggg ctggccggaa gcgccagtta cagcagcggg gtcttcaccg tgcgcggcgc
     3241 cggcgccgac atctggggag ccgccgactc cttcaacttt gcgaaccaga ttctctccgg
     3301 cgacgggcag gtcgtcgtcc gcgtcgcctc gatggggaat acacacacct acgcgaaggc
     3361 cggggtgatg ttccgggagt cgctcaccgc cggctcccgc cacgtcgtcc tcgacatgaa
     3421 gccgggcggc ggcgtcgagt tcatggcgcg aagcgtcacg ggcggctcga cgtcctacat
     3481 cggagggggg gcgggccgtt ggctgaagct ggtgcgccgc ggcagcacct tcacggcgta
     3541 cgtgtctgcc gacggcgctg cgtggacttc gatcgggagc cggtcggtaa ccatggcagc
     3601 caatgcctgg gtagggctcg tcgtctgcag tcacaacacc ggtgtgctga acacggccac
     3661 cttcgaccgg gtcacggtgg gagatggcga tctcccaccg gccgtggcga tcacgagccc
     3721 ggcagccggg gcagccttca gcgcaggggc ggacgtcacc gtgacggcca acgccaccga
     3781 taccgatgcg acgcccgtcg ccgaggtcga gttctacgcc aacggctcgc tcatcggacg
     3841 cgacaccagc agtccctaca gtgtcacctg ggccgacgtg ccggccggat cctactcgct
     3901 cacggcggtg gccactgaca ccgccaacct gtcggcgacc tcacgggccg ttgccgtcac
     3961 ggtctcgggc cctaccgggg cgctgccaac cggctgggcg gacaccgata tcggaaccgt
     4021 gggtctggcc ggagccgcga gccacaacgc cgggacgttc acggtacggg gcgcgggggc
     4081 cgacatctgg ggcacggcag actccttcca cttcgccaac cgcgctctct cgggggacgg
     4141 gcagatcacc gcgcgcgttg cctcgatgga tagcacgcac gcctatgcga aggcgggcgt
     4201 gatgatccgc gagacgctga cggccgggtc gcgtcacgtg ctcctcgaca tgaagccgtc
     4261 gggcggcgtc gagttcatgg cccgggcggc gacgggcggc tccacgtcgt acatcgccgg
     4321 cggcacgggt cgctggctgc ggctcgtgcg cagcggcagc accttcacgg cttacgtctc
     4381 cgccgatgga gccacgtggc gatccatcgg cagccgctcg gtgacgatga acgccacggt
     4441 ctgggtcggg ttggccgtct gcagcca
//
LOCUS       DAOPQX010000315         4462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21206_length_4462_cov_4.902655, whole genome shotgun sequence.
ACCESSION   DAOPQX010000315 DAOPQX010000000
VERSION     DAOPQX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21206_length_4462_cov_4.902655"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKK95_14065"
     CDS             <1..345
                     /locus_tag="PKK95_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOC19394.1"
                     /translation="LVGFAFVVAILDLIMTGAIPKWAIFAPIFVPLLMRLGVDPEAVL
                     AAYRVGDSPMNAVSPMNAYFGLIVAFAMKYQKDAGVGTVIALMLPYVAVLFVLWPLLL
                     AVWHVLGLPWGV"
     gene            complement(366..1718)
                     /gene="pepT"
                     /locus_tag="PKK95_14070"
     CDS             complement(366..1718)
                     /gene="pepT"
                     /locus_tag="PKK95_14070"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOC19395.1"
                     /translation="MGKQTAEAGATPAAVQQRGFTRELEERLLRYVRIDTQSDDSSAT
                     SPSTARQYDLLNLLVEELRSMGAQDVRLTGYGVVLATIPPTVPADCPVIALLAHVDTA
                     PAFNATGVKPIVHRNYDGADIVLPDEPGVVLSPSQFPYLAAKVGDDIVTASGTTLLGA
                     DDKAGVAIVMTAVRHLLENPGLAHGPLRVAFTPDEEIGRGVHSSLPADLGADVAYTLD
                     GAGLGEIVFETFSADRADVHVKGVSIHPGQAKDKLVNALHLAAKILDTLPHVTLTPET
                     TEGRQGFIHAYQLNGTAAAADLHFALRDFELQGLRERGELVTKICAAIEATEPRARIT
                     CSITPQYRNMRYWLEDDMRPVDLARESCRRIGIEPFTQPIRGGTDGSRLTEMGVPTPN
                     LFTGMQNIHGPHEWVSLQDMAHATAMCVKLAELWAGERRGAAASRATPLEAAPVDWAG
                     "
     gene            1900..2559
                     /locus_tag="PKK95_14075"
     CDS             1900..2559
                     /locus_tag="PKK95_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19396.1"
                     /translation="MTPDLVSAAAMKRWALRLRRSIDLFSAVVMAGATVATAYSAYQS
                     SLWSSEKNRHTAQAATAVVRVGKLSNMALQRTSVHVNLFVHWVSAVRRGDGGMAAYLF
                     DRFPEPLKAAASAWRATRPLENPAAPASPFEMPEYVLQERTDADRWEESAQRETAAAH
                     HADSQSSRYLLFTIIYASVLFFGGISGKFRKPAIDLLVLALGGLTLLGCVAIMLSSPR
                     L"
     gene            complement(2721..>4462)
                     /locus_tag="PKK95_14080"
     CDS             complement(2721..>4462)
                     /locus_tag="PKK95_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOC19397.1"
                     /translation="ALASDSILDLRRGDADDDRTSPYGRGWRSIVISTALESNYLTAS
                     IAFVALIIVPALLVGIAAPLLLAYGRSTLGAVAVIRSHPFAALASLAVLVGLALSAGR
                     PLAAKAVENLWHLHYTLVLPFFVGLRETISAAIERLPGQTHASAQLDRRRRLATVLAT
                     LLLAACGILLAASVGALAHPGLVDVVRQPRAVARAGFGNAAVVLGVSTALASVYWLWR
                     ELAGRPPLRDWTAEPPAGQGRMVRVAHLSDLHVVGERYGYRMECGTHGPRGNGSIRHA
                     LDKLQAIHASSPIERVLVSGDVTDAGTRAEWAEFLDLLRCRPELRARALFVPGNHDLN
                     IVDRTNTGRADIPWSVGHALRRLRFVVALDAIQGERVHVVRAAGALGPSLRGYLREGE
                     RPARLRGLAERGTWRGRWEIAQVWSDAFPLVAPPRDGGTGVILLDSNARSHFSATNAV
                     GVVSRLQLKRLAGILDAFPDSGWIVVLHHHVAEYPVASVKLADRIALSLMNAPDVLSA
                     VGRHCSRVVILHGHRHHDWIGTSGGLVLCSAPSVSLGSVGSDLYRGSFHINELALSNG
                     GMRLATSQRVFFE"
BASE COUNT          700 a         1525 c         1542 g          695 t
ORIGIN      
        1 ctcgtcgggt tcgccttcgt cgtcgcgatc ctcgacctga tcatgaccgg tgcgatcccg
       61 aaatgggcga tcttcgctcc gatcttcgtg ccgctgctca tgcggctggg cgtcgatccc
      121 gaagccgtgc tggcggcgta ccgggtgggt gactcgccca tgaacgccgt ctcgccgatg
      181 aacgcctact tcggcctgat cgtggccttc gccatgaagt accagaagga cgcgggcgtg
      241 ggcacggtga tcgcgctgat gctgccctac gttgccgtgc tcttcgtgct gtggccgctg
      301 ctgctggcgg tctggcacgt cctcgggctt ccttggggcg tctgacgatc gagggacgcg
      361 caggatcaac cggcccaatc taccggcgct gcctccagcg gcgtcgcccg gctggcggca
      421 gccccgcgcc gctcgcccgc ccatagctcg gcaagcttga cgcacatcgc cgtggcgtga
      481 gccatgtcct gcaggctcac ccactcgtgc gggccgtgga tgttctgcat gcccgtgaac
      541 aggttgggcg tcggcacacc catctcggtc aggcgggagc cgtcggtgcc gccgcgaatg
      601 ggctgggtga agggctcgat gccaatccgc cggcagctct cccgcgcgag atcgaccggc
      661 cgcatgtcgt cttcgagcca gtaccgcatg ttgcggtact gcggcgtgat ggagcaggtg
      721 atccgcgcac gcggctcggt cgcctcgatc gcggcgcaga tcttcgtgac cagctcgccg
      781 cgctcgcgca agccctgcaa ctcgaagtcg cgcagcgcga agtggaggtc ggccgctgcg
      841 gccgtcccgt tcaactggta ggcgtggatg aacccctgcc ggccctccgt cgtttccggt
      901 gtcagcgtga cgtgaggcag ggtgtccaag atcttcgccg ccaggtgcag cgcgttgacc
      961 agcttgtcct tggcctgccc gggatggatc gagactccct tgacgtgcac gtcagcccgg
     1021 tcggccgaga acgtctcaaa gacgatttcg ccaagcccgg cgccatccag cgtataggcg
     1081 acgtcggcgc cgaggtccgc gggcagactg ctgtgaactc cccgcccgat ctcctcgtcg
     1141 ggtgtgaacg cgacgcggag cggcccgtgc gcgaggcctg gattctccag caggtggcgc
     1201 accgcggtca tgacgatcgc cacgcccgcc ttgtcgtcgg cgccgaggag cgtcgtcccg
     1261 ctggcggtga cgatgtcgtc gcccaccttc gccgcaagat aggggaactg actcggcgac
     1321 agcacgacgc cgggctcgtc gggcagcacg atgtcggccc cgtcgtagtt gcgatgaacg
     1381 atcggcttga cacccgtggc gttgaacgcc ggcgccgtat cgacgtgcgc cagcagcgcg
     1441 atgacggggc aatcggccgg gacggtcggg gggatcgtcg ccaggaccac gccatagccg
     1501 gtcagcctga cgtcctgggc ccccatggat ctcagttcct cgacgagcag gttcagcagg
     1561 tcgtactgcc tggcggtgct cggcgacgtc gctgagctgt cgtcgctctg ggtgtcgatc
     1621 cgcacgtagc gcagcaggcg ctcctcgagc tctcgcgtga agccccgctg ctgaacggcg
     1681 gccggcgtgg cgccggcctc tgctgtctgc ttgcccatga cgctcgcctc ctccggttgt
     1741 cgacgtgtac gccgcgtgcg atgcggccgg cgccaggcct cgaaagggaa gaaaatccgt
     1801 atcaacattc gcgccatggg cagcccaggc tcgacagccc ttcctggcac gcgacctgcg
     1861 aagtccttgc cgtcgcgcgc ttcaggagaa gtctcaccca tgactccaga cctcgtgtca
     1921 gcagccgcca tgaagcggtg ggcgttgcgg ttgcgccggt cgatcgacct gttttctgca
     1981 gtcgtgatgg ctggggccac ggtggcgacg gcgtacagcg cctatcagtc gtccctgtgg
     2041 agcagcgaga agaacaggca cacggcgcag gcggcgacgg ccgtggtcag ggtggggaag
     2101 ttgagcaaca tggccctgca gagaacgtcg gtccacgtca acctcttcgt gcactgggtc
     2161 tcggccgtcc gcaggggcga cggcggcatg gccgcctacc tgttcgatcg cttccccgag
     2221 ccgctgaagg cggccgccag cgcctggcgg gcaaccaggc cgctcgagaa tcccgcggcg
     2281 cccgcctcgc cgttcgagat gcccgagtac gtccttcagg agaggaccga tgccgaccgc
     2341 tgggaggagt ccgcacagcg ggagacggcc gcggcccacc acgccgactc gcagtcgagc
     2401 cgctacctgc tcttcacgat catctacgcc tccgtgctgt tctttggagg gatcagcggt
     2461 aagttccgga agcctgcgat cgacctgctg gtgctcgcgc tggggggcct gacgctgctc
     2521 ggctgcgtgg ccatcatgct gtcgtcgccg cgcctctgag ccttgtgatg agacgcacgc
     2581 aacagacgga acggcttgtc cacgtcaacc acagaggcac ggagacgcag agacgtacgc
     2641 aacctcggat ctttcaaaga tccctccgtt cttgcattgc ctagggtgct ggggctgcac
     2701 ctcctgcacc accggcacga tcattcgaag aacacccgtt gcgacgtcgc cagccgcatt
     2761 ccgccgttgg acagggccag ctcgttgatg tgaaaactgc cgcggtagag gtcggacccg
     2821 accgagccga gcgacaccga cggcgccgag cacagcacga ggccgccgct ggtgccgatc
     2881 cagtcatggt gtcgatggcc gtgcaggatc accacgcgcg agcagtgccg cccgaccgct
     2941 gacagcacgt cgggagcgtt catcagcgac agggcgatcc gatcggccag cttgacagag
     3001 gctaccgggt actcggcgac gtggtggtgc agcacgacga tccagccgct gtcgggaaac
     3061 gcgtccagga ttccggcgag ccgtttcagc tgcagccggc tgaccacgcc aaccgcattg
     3121 gtcgccgaga agtggctgcg ggcattcgaa tcgagcagga tcacgccggt tcccccatcg
     3181 cggggcggcg cgaccagcgg gaaggcatcc gaccacacct gggcaatctc ccatcgcccg
     3241 cgccaggtgc cgcgctccgc gagcccccgg agtcgtgccg gccgctcgcc ttcccgcaag
     3301 tagccgcgca gggaggggcc gagggcgcca gcggcgcgca cgacgtggac gcgctcaccc
     3361 tggatcgcgt cgagcgcaac cacgaacctg agacgccgca gcgcgtggcc gacgctccag
     3421 gggatgtcgg cgcgcccggt gttcgtgcga tcgacgatgt tgaggtcgtg gttgcccggg
     3481 acgaagaggg cgcgcgctcg aagctcaggc cggcaccgga gcagatcgag gaactccgcc
     3541 cattcggcgc gcgtgccggc gtcggtgacg tcgccgctga ccagtacgcg ctcgattgga
     3601 gaggacgcgt ggatcgcctg gagcttgtcg agcgcgtgcc ggatgctgcc gttgccccgg
     3661 ggaccgtgcg tgccgcattc catgcggtag ccgtagcgct cgccgacaac gtggaggtcg
     3721 gacaggtgcg ccacccgcac catccgcccc tgaccggctg gcggctccgc agtccagtcg
     3781 cggagcggcg gccgccccgc aagctcgcgc cacagccaat acacgctcgc gagggcggtg
     3841 gacaccccca ggaccacggc ggcattgccg aaaccggccc tcgccaccgc ccggggctgc
     3901 ctcaccacgt cgacgagccc ggggtgcgcc agcgccccga cgctcgcagc caggagaatc
     3961 ccgcaggccg ccagcagcag cgttgcgagc accgtcgcaa gccggcggcg ccgatcgagc
     4021 tgcgccgagg cgtgcgtctg cccgggcagc cgttcgatgg cggcgctgat cgtttcgcgc
     4081 agcccgacga agaacggcag gacgagggtg tagtgaaggt gccagaggtt ctcgacggcc
     4141 ttggccgcga gcggccggcc ggccgacagg gccaggccga ccagcacggc gagcgaggcc
     4201 agggcggcga acggatggct gcggatcacg gcgacagcgc cgagcgtgga ccggccgtac
     4261 gcgagcagca aaggcgccgc gataccgacg agaagcgccg gcacgatgat gagcgcgacg
     4321 aaggcgatcg atgccgtgag gtagttcgac tcgagcgccg tcgaaatcac gatgctccgc
     4381 cagccccggc cgtaggggct cgtccgatca tcgtcggcat cgccgcggcg gagatccagg
     4441 atggaatccg atgccagggc tt
//
LOCUS       DAOPQX010000316         4444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21318_length_4444_cov_3.443837, whole genome shotgun sequence.
ACCESSION   DAOPQX010000316 DAOPQX010000000
VERSION     DAOPQX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21318_length_4444_cov_3.443837"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PKK95_14085"
     CDS             <1..581
                     /locus_tag="PKK95_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19398.1"
                     /translation="QFGGSLVVSRMAGNYPGTSGAPTGFFRTPNYRVNRDGRIGVDQP
                     VQVKLWGSAMLPFGMQASYFYRYLSGTPWNRTVTVIPPADWAAANGTATFGEAVNLEV
                     PGTRRHTSTSNLDLRLEKVFKLGGKRQVGAFVDLFNALGFVYPSATVNPGGTWRPDGV
                     GTPEGRYTPGAMRLTGLSGGVRTIKLSARIAF"
     gene            684..1463
                     /locus_tag="PKK95_14090"
     CDS             684..1463
                     /locus_tag="PKK95_14090"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOC19399.1"
                     /translation="MASTGRSSGSYNCLRGVTGQVYSRLCALISSGALAPGAPLIETD
                     LSLRLKVSRTPVRAALLKLQQEGLVHVTRVGQLARAVVAPLTADDVQELHYVVGALEG
                     LAARRAAELTSRQRASVAEAMEHINARLTAATAARDLRTAQDLHVRFHRCYVEAVAGP
                     RLLAELDGLQPQIERYERFYTHALINEHRFAEALREHEAIIAAIRQGSPDAAERAVAA
                     NWRNGARRYAPIVMRAGERGSWAGDGDARGNKEGSTWAVVR"
     gene            1460..3553
                     /locus_tag="PKK95_14095"
     CDS             1460..3553
                     /locus_tag="PKK95_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOC19400.1"
                     /translation="MTRRGFITTSAAGLGAAALGARGTQGSPAEPGLAARWDFESLRE
                     DAVRETIGGVDDAIEGAFQATRGVAGQALKLDGFSTCVIRKADRAPRLEGEFTLAAWV
                     ALAARPWNWCPVVSQRDGEDRGYCLTIGPRGQASLQLSLDGRWETCTSPDFTIPLREW
                     VHLAATYNSGRGLALYVNGRLAASSPAGGRVEYARDVDLRIGMNHRKVKPSDIHREFG
                     TRPYWFSLDGVLDDVRIYSRALSAHEVHSLHGARVPTEPPDLKPRRLPSGPPGPGRFG
                     AYYCNLKYYEEWDALWPVGPDPDVVVRFDRTAARVVFWRGSRYSPAWVSENELWMADQ
                     SVEAWDDVEGCFEHMQDRHCRHSQVRVIESHDARVVVHWRYAPISAHDHLWRVDEKTG
                     WPCWVDEYYYIYPDATAVRFVSWKQDSLGQPRQFQESLPFTHPGQLPGEVVHPDWVTI
                     GNLQGETGVLSFIENPPKVKVKPGLPADLTIQRYNFRADNKPFIVFEPGNRMQYLWDR
                     DIRALSRPGACDHWPVGQNPCDGRTAQTSDHPTHFLGFPISDPPVHEGGGRCWWHALY
                     GMSTGAFADLVKLARSWNQPAALRVAGTGATSEGYSKSERAYHVACAPGGPKGAIELD
                     LAGSDASPIVNPAFLVRNWGEAGADISIDGRRTAPGPNLRVGVRRGLEGTHLLVWVAL
                     ESSGSVKMRIVPRVG"
     gene            complement(3592..>4444)
                     /locus_tag="PKK95_14100"
     CDS             complement(3592..>4444)
                     /locus_tag="PKK95_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19401.1"
                     /translation="DVFFDEVTGSNSSGTALVAGSTIDMQILTDLFGQVAEAARILGL
                     DAGFRTRVLDARAKLAPMQIGRNGDLQEWLDDWGQREKSHRHISNLWGLFPGTQVSPR
                     KTPSLAAAAAVVLEQRGLPGNGWSSAWKAASWARLGNGAKAMDNFTFAMKNYTTTSLF
                     SICSGAMQVDGAFGMTAAIAEMLIQSHEGELLVLPALPATWAEGEVTGLRARGGYEVG
                     LAWKEGRLTRLRIASLNGNRCRVRAAASLVVTLNGRPVAATTPEPGVIEFQTTVGAAY
                     VLTPTAR"
BASE COUNT          680 a         1538 c         1537 g          689 t
ORIGIN      
        1 ggcagttcgg gggatccctg gtggtgtccc gcatggcggg caactacccg ggaaccagcg
       61 gggcgccgac cgggttcttc cgcacaccga actaccgagt caaccgtgac ggccgtatcg
      121 gcgtcgacca gccggtccag gtcaagctgt gggggagcgc catgctgccc ttcgggatgc
      181 aggcgtccta tttctaccgt tacctcagtg gtacaccgtg gaaccgcacg gtgaccgtca
      241 ttccccccgc cgattgggca gcggccaatg gcacggccac cttcggcgag gcggtcaacc
      301 tggaggtgcc gggtacccgg cgtcacacct cgacgtccaa tcttgacctg cggctcgaga
      361 aggtcttcaa gttgggaggg aagcgccagg tgggcgcgtt cgtggacctg ttcaacgcgt
      421 tgggattcgt ctacccgtca gcgacggtca atcctggcgg gacctggcgg cccgacggcg
      481 tcggcacccc ggaaggccga tacacgccgg gggcaatgcg cctcacgggc ctgagcggcg
      541 gcgtccgcac catcaagctg tcggcgcgga tcgccttcta gcgcagaggg ctggcggggg
      601 cgggggagcc taccccgtcc ccgccccggt ctacgcgtcc ctcctcggag cgaggagtca
      661 tgcctcgtcg ccttcccaga cgcgtggcct ccacgggccg cagctccggc agctacaact
      721 gcctgcgcgg cgtgaccggt caggtctact ctcgactgtg cgcgctgatt tcctcgggtg
      781 cactggcccc gggtgccccc ctgattgaga ccgacctctc gctccgccta aaggtcagcc
      841 gcacccccgt ccgcgccgcc ctgcttaagc tgcagcagga gggcctggtg cacgtcacgc
      901 gggtcgggca actggcgcgc gcggtggttg cgccgctgac cgccgacgac gtgcaggaac
      961 tgcactacgt cgtgggcgct ctcgaagggc tcgcggcccg gcgggccgcc gaactcacga
     1021 gccgccagcg ggcgtcggtg gctgaggcga tggagcacat caacgcgcgc ctcaccgcgg
     1081 cgactgcggc gcgcgacctg cggacggcgc aggatctgca cgtgcggttc caccgctgtt
     1141 acgtcgaggc ggtggccggt cctcgcctgc tcgccgagct cgacgggctg cagccgcaga
     1201 tcgagcgcta cgagcgcttc tacacgcacg ccttgatcaa cgagcatcgg ttcgcggagg
     1261 ctttgcgcga gcacgaggcg atcatcgccg ccatccggca gggtagtccc gatgccgccg
     1321 agcgcgccgt tgcggccaac tggcgcaacg gcgcgcgccg gtacgccccg atcgtgatgc
     1381 gcgccggcga gcggggcagt tgggcgggcg acggggacgc ccgaggaaac aaggaagggt
     1441 cgacatgggc agtggttcga tgacgcgtcg cggattcatc acgacctcgg ccgcggggct
     1501 cggcgctgcg gcgctggggg cacgaggcac ccaaggcagc ccggccgaac ccggtctggc
     1561 cgctcgctgg gacttcgaaa gccttcgcga ggatgcggtc cgcgagacga tcggcggggt
     1621 ggacgacgcc atcgagggag cgttccaagc gactcgcggc gttgccgggc aggcgctgaa
     1681 gctcgatggc ttctcgacgt gcgtgatcag gaaagcggat cgggcgccgc gcctcgaggg
     1741 cgagttcacc ctggccgcct gggtcgccct tgccgcccgg ccgtggaact ggtgccccgt
     1801 cgtgagccag cgcgacggtg aggaccgcgg ctactgcctg acgatcggcc cccgcggcca
     1861 ggccagcctg cagctctcgc tcgacggccg gtgggagacg tgcacctcgc ccgacttcac
     1921 catccccctt cgggaatggg tgcacttggc ggcgacctac aacagcggac gagggctcgc
     1981 gctgtacgtg aatggccgcc tcgcggcaag ttccccggcc ggcggccgcg tcgagtacgc
     2041 ccgcgatgtc gatctccgga ttggcatgaa ccaccggaag gtgaaaccat ccgacatcca
     2101 ccgcgagttc gggacgcgcc cctactggtt ctcgctcgac ggcgtcctcg acgacgtccg
     2161 gatctactcg cgggcgctga gcgcccacga ggtgcactcc ctccacggcg cccgggttcc
     2221 caccgagccg cctgatctga agccccgacg gctcccgtcg ggaccgcccg gcccgggccg
     2281 gttcggcgcc tactactgca atctgaagta ctacgaggag tgggacgccc tctggccggt
     2341 cggccccgat cccgacgtgg tcgtgcgctt cgaccgcacg gccgcgcgcg tcgtattctg
     2401 gcgagggagc cggtattccc cggcgtgggt ctctgagaac gagctgtgga tggccgacca
     2461 gagcgtcgag gcgtgggacg acgtcgaggg ctgcttcgaa cacatgcagg atcgacattg
     2521 ccgccactcc caggtgaggg tgatcgagag ccacgatgca cgggtcgtgg tgcattggcg
     2581 ctacgcgccg atcagcgccc acgatcacct gtggcgcgtc gacgagaaga ccggctggcc
     2641 gtgctgggtg gacgagtact actacatcta ccccgacgcc acggcggtcc gtttcgtgag
     2701 ctggaagcag gacagcctcg ggcagccccg gcagttccag gaatcactgc cgtttaccca
     2761 ccctggacag ctgccaggcg aggtcgtgca ccccgactgg gtcaccatcg ggaacctcca
     2821 gggagagacc ggggtgttgt cgttcatcga gaaccccccc aaggtgaagg tcaagccggg
     2881 cctccctgcc gacctgacga tccagcgcta caacttcagg gctgacaaca agccgttcat
     2941 cgtcttcgag cccggcaacc ggatgcagta cctctgggat cgcgacatcc gcgccctgtc
     3001 gcggccgggt gcgtgcgatc attggccagt cggccagaat ccctgcgacg ggcggaccgc
     3061 gcagaccagc gaccatccga cgcacttcct cggcttcccg atctcggatc cgccggtgca
     3121 cgagggtgga gggcgctgct ggtggcacgc cctgtacggc atgtcgaccg gtgcattcgc
     3181 agatctcgtc aagctcgcgc gcagctggaa ccagccggcg gcgttgcgcg tggccggcac
     3241 cggggcgacg agcgagggct acagcaagag cgagcgggcc taccacgtcg cgtgcgcgcc
     3301 gggcgggccg aagggtgcga tcgagctgga tctcgccggc agcgacgcgt cgccgatcgt
     3361 caatcctgct ttcctggtga ggaactgggg cgaggcaggc gccgacatct ccatcgacgg
     3421 ccgcagaacc gcgccgggcc caaacctgcg agtgggggtg cgtcgggggc tggaggggac
     3481 ccatctgctc gtctgggtcg ccctcgagtc gtccgggtcg gtcaagatgc gaatcgtccc
     3541 gagagttggc tgacccccgc ccctggcgct accacggccg cggcgcttca ctcaccgggc
     3601 agtcggggtg agcacgtagg cggcgccgac cgtcgtctgg aactcgatga ctcctggctc
     3661 tggcgtcgtc gccgcgacgg ggcggccgtt gagcgtgacg accagcgacg cggctgcgcg
     3721 aacgcgacag cggttgccgt tgagggacgc gatcctcagg cgggtgagcc gtccctcctt
     3781 ccacgcgagt ccaacctcgt acccgccgcg cgctcgaagc ccggtgacct cgccttccgc
     3841 ccaagtcgcc gggagggcgg gcagcaccaa cagttcgccc tcatgtgact gaatcagcat
     3901 ctcggcgatg gccgccgtca tgccgaacgc gccgtcaacc tgcatcgccc ccgagcaaat
     3961 cgagaacagg ctggtggtgg tgtagttctt catcgcgaag gtgaagttgt ccatcgcctt
     4021 cgcgccgttc cccaggcgag cccacgaggc cgccttccag gccgatgacc agccgttgcc
     4081 aggcaggccg cgttgctcca gcacgacggc tgccgcggcg gcgaggctcg gcgtcttgcg
     4141 gggcgacacc tgggttcccg ggaaaaggcc ccacaagttg gagatgtgcc gatggctctt
     4201 ctcgcgctgc ccccagtcgt cgagccactc ctgcaggtcg ccgttacgtc cgatctgcat
     4261 cggtgccagc tttgcccgcg catcgaggac ccgcgttcgg aacccggcat cgaggcctag
     4321 gatgcgggcc gcctcggcca cctggccgaa caggtccgtc aggatctgca tgtcgatggt
     4381 cgaccccgca accagcgcgg ttccactgct gttgctgccg gtcacctcgt cgaagaagac
     4441 gtcg
//
LOCUS       DAOPQX010000317         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21322_length_4443_cov_4.149727, whole genome shotgun sequence.
ACCESSION   DAOPQX010000317 DAOPQX010000000
VERSION     DAOPQX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21322_length_4443_cov_4.149727"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1203)
                     /gene="argG"
                     /locus_tag="PKK95_14105"
     CDS             complement(16..1203)
                     /gene="argG"
                     /locus_tag="PKK95_14105"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF012966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOC19402.1"
                     /translation="MNDVLLAFSGGLDTSFCVVWLASKGWKVHTVTVDTGGFAPGETS
                     RLEQWALQLGAATHETVDARQELFDRYLRYLVAGNVLRGGVYPLSVSAERVCQARRIV
                     EAARARGARAIAHGSTGAGNDQVRFDVAFRALAPDLEVLAPIRELAPSRAEERAFLAE
                     RGFTMSEKTEAYSVNEGMWGSSVGGRETHDSWDELPDEAFPGGAIDPLLPPSTLTISF
                     ERGVPSAIDGTATAPVALIGALNAIGGRYGIGRGVHLGDTILGIKGRVGFDAPAAHLL
                     ITAHRELEKLVLTGRQLAWKETLGALYGSLLHEGLFFDPVARDIEAFLESSQARVTGD
                     VRCILRPRSLAVAGVRSPHSLMTPDAKYGEGSGLWRGEEAAAFSKIYGLAQALALRAG
                     GTA"
     gene            complement(1208..2278)
                     /locus_tag="PKK95_14110"
     CDS             complement(1208..2278)
                     /locus_tag="PKK95_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC19403.1"
                     /translation="MRAVDLLPALVAVPSVSRGEKAIADLLASYLAASGVTVNRAGHN
                     LWFEVGQGRPRLLLASHLDTVPACAGWSRDPHVARWDGDRLVGLGANDAKGCVAAMTE
                     AALALRAEGATLPGSVVVALTVEEEVGGQGIATIRPLLGEIDAAVIGEPTGLEVCVAQ
                     RGLVILRCVARGRGGHAAHAAQAVNAVHVAAADIGRLAGLVFGRHPRLGDTTAQVTRI
                     EGGRALNQVPDTCEFFVDLRTVPGITVESIVEQLRGEIASEVHVNSSRYLPCATDEAH
                     PVVQAALAAAGRERGTVSRTTSDWAFLSDLPAVKAGPGDTTRSHRPDEYITRQELGDG
                     VAFYRRLVPECLARLAAVPVKD"
     gene            complement(2275..3174)
                     /gene="argB"
                     /locus_tag="PKK95_14115"
     CDS             complement(2275..3174)
                     /gene="argB"
                     /locus_tag="PKK95_14115"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOC19404.1"
                     /translation="MPFAPDPFSTVRSVARHVRRFRGKTFVVKIGGELIDDAECRASV
                     SEQLSLLWSFSIPIVIVHGGGGAVDALCGSLGLATEKVAGRRVTPPEVLRAAGMALKG
                     DAQVRLVSSLLSAGLPAVGLTGQDAGLVTAARRPPVVMDGRLVDYGEVGDITAVDPTL
                     VTRLIEGGFVPVVAPLTGGADGAVYNTNADSVAASLAVALGAEKLLFLVRVPGLLQDR
                     DNPASLVPFADLAAVDQLVASGAVSGGMRPKLDAARLALEGGVTSVHLVSGVGADNLL
                     VEVFTNEGAGTMLVRQADRGAPA"
     gene            complement(3178..4167)
                     /locus_tag="PKK95_14120"
     CDS             complement(3178..4167)
                     /locus_tag="PKK95_14120"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HOC19405.1"
                     /translation="MRHFTCLADLGPDGVHAVLDQAIAWKRCPPEAHLAGKILGMVFF
                     NPSLRTRASFEAVMARGGGHAIVLEVGGGVWKLEDRIGAVMDQDRAEHVREAVPVLGR
                     YVDLLAVRTFASCTNDDDDERDPVMRAFADYSTVPVISMESAREHPCQGLADVLTIAE
                     TFGETRGLPVTLTWAPHVKPLPKAVPHSFLLTAGAAGCRVRVAHPPGFELHPAVVSEA
                     REFARASGGEVTFTSDQDEALEGSRVVYAKAWGPAAPGPLGPEAVRAFPSWRIEARQL
                     ARAASDAIFLHCLPVRRNVEVGDAVLDSPASRVIDEAENRFHVQRMLLDWLAR"
     gene            complement(4172..>4443)
                     /locus_tag="PKK95_14125"
     CDS             complement(4172..>4443)
                     /locus_tag="PKK95_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19406.1"
                     /translation="LMERARVCGERIRAGVAGTAVTAVHGRGLLLGLRVPGRAGELKT
                     FLQDKRILVGGSADPDVLRLMPPLTVRDSSIDALIEAIASFAAER"
BASE COUNT          648 a         1647 c         1553 g          595 t
ORIGIN      
        1 tgtccaggtg caccctcacg ccgtgcctcc tgcccgcagg gccagcgcct gcgccagccc
       61 gtagatcttc gagaaggcgg ccgcctcctc gccccgccag aggcccgagc cctcgccgta
      121 cttggcgtcg ggcgtcatca gcgagtgggg gctgcgaacg ccggccaccg cgagcgagcg
      181 tggccgcagg atgcagcgga cgtcgccggt gacgcgagcc tggctgctct ccagaaacgc
      241 ctcgatgtcg cgcgcgacgg ggtcgaagaa cagtccctcg tgcaggaggc tgccgtagag
      301 ggcgccaagc gtttctttcc acgcgagctg ccggccggtg agcaccagct tctcgagctc
      361 gcggtgcgcc gtgatcagca ggtgggctgc cggcgcgtcg aaaccgacgc gccccttgat
      421 cccgaggatg gtgtctccaa gatgcacgcc ccgcccgatg ccgtatcgtc cgccaatcgc
      481 gttgagcgct ccgatgagcg ccaccggggc cgtcgccgtg ccgtcgatcg ccgaggggac
      541 gccgcgctcg aacgagatcg tcagcgtcga ggggggcagc agcgggtcga tggcgccgcc
      601 ggggaaggcc tcgtccggca gctcgtccca gctgtcgtgg gtttcgcgcc cgccgacgct
      661 cgagccccac atgccctcgt tcaccgagta ggcctcggtc ttctcgctca tcgtgaagcc
      721 gcgctcggcg aggaacgcac gctcttcggc gcggctcggt gccagttccc tgatcggcgc
      781 cagcacctcg aggtccggcg cgagcgcgcg gaaggccacg tcgaaccgca cctggtcgtt
      841 tcccgcgccg gtggacccgt gggcgattgc cctggctccg cgggcgcggg cggcctcgac
      901 gatcctgcgc gcctggcaca ctcgctccgc cgatacgctc agcgggtaga cgccgccgcg
      961 caagacgttt ccggcgacga ggtaccggag gtaccggtcg aacagctcct gcctggcatc
     1021 caccgtttcg tgcgtggctg ccccgagctg aagcgcccac tgctcgagcc tcgacgtttc
     1081 tccgggcgcg aacccgccgg tgtccacggt gaccgtgtgg accttccatc ccttcgacgc
     1141 cagccagacg acgcagaagg acgtgtcgag gccgcccgag aaggcaagaa gcacgtcgtt
     1201 catcggttca atccttgaca ggcacggccg cgagccgcgc gaggcattca gggaccaggc
     1261 ggcggtagaa cgcgacaccg tcgcccagct cctgccgcgt gatgtactcg tcgggccggt
     1321 ggctgcgcgt cgtgtcgccg ggcccggcct tgactgccgg caggtccgac aggaacgccc
     1381 agtccgaggt cgtgcggctg accgttcccc gctcgcgccc ggccgccgcg agcgccgcct
     1441 gcaccacggg gtgcgcctcg tcggtggcgc acggcaggta gcgcgaggaa ttgacgtgaa
     1501 cctcgctggc gatctcgccg cgaagctgct cgacgatcga ctcgaccgtg atgcccggca
     1561 ccgtgcggag atcgacgaag aactcgcacg tgtccggcac ctggttcagg gcgcggccgc
     1621 cttcgatcct cgtgacctgc gcggtggtgt cgccaagtcg cgggtgccgg ccgaacacga
     1681 ggcctgccag gcggccgatg tcggcggccg ccacgtggac ggcgttgacg gcctgcgcgg
     1741 cgtgcgcagc gtggccgccg cgcccccgcg ccacgcaccg caggatcacc aggccgcgct
     1801 gcgcgacgca gacctcgagt cccgtcggtt ctccgatcac cgcggcgtcg atctcgccga
     1861 gcagcgggcg tatcgtcgcg attccctggc cgcccacctc ctcctccacc gtgagggcga
     1921 cgaccaccga ccccggcagc gtcgcgcctt cggcccgcag cgcgagcgcc gcctcggtca
     1981 tcgcggccac gcaccccttg gcgtcgttgg cacccaggcc cacgagccgg tcgccgtccc
     2041 agcgtgccac gtgcgggtcg cggctccacc ccgcgcaggc cggcacggtg tcgaggtggc
     2101 ttgcgagcag cagccgcggc cttccctggc cgacctcgaa ccagagattg tggccggccc
     2161 ggttcaccgt cacgccactc gccgccaggt acgacgccag gaggtcggcg atcgccttct
     2221 cgccgcgcga gacgctcggg acggcgacca gcgccggcag caggtccacg gccctcacgc
     2281 cggcgcccca cgatcggcct gacgcacgag catggtcccg gccccctcgt tggtgaagac
     2341 ctcgaccagc aggttgtcgg cgccgacgcc ggacacgagg tggacgctcg tcacgccgcc
     2401 ctcgagggcc aatcgcgccg cgtccagctt gggccgcatg ccgccgctca ccgcccccga
     2461 ggcgacgagc tggtccaccg ccgcgagatc ggcgaacggg acaagggaag ccgggttgtc
     2521 gcggtcctgg agcagccccg ggacgcgcac gaggaagagc agcttctcgg cgccgagcgc
     2581 gacggcgagc gatgcggcca ccgagtcggc gttcgtgttg tagacggcgc catcggcccc
     2641 gccggtcagc ggcgccacca ccgggacgaa gccgccttcg atcaggcgcg tcaccagcgt
     2701 ggggtcgacc gccgtgatgt cgccgacctc gccgtagtcg accaggcggc cgtccatcac
     2761 gaccgggggt cgccgggcgg cggtgacgag gcccgcatcc tggcccgtca ggccgacggc
     2821 gggaaggccg gccgacaaca gcgaggagac caggcgcacc tgcgcgtccc ccttgagggc
     2881 catgccggcg gcccgcagca cctcgggcgg ggtgacgcgg cgccccgcca ccttctcggt
     2941 cgcgaggccg agcgagccgc acagcgcgtc cacggcgccc ccgccgccgt gcacgatcac
     3001 gatcgggatc gagaagctcc agagcagcga cagctgttcg ctcaccgagg cgcggcactc
     3061 ggcgtcgtcg atcagctcgc cgccgatctt cacgacgaag gtcttccctc gaaagcggcg
     3121 cacgtgcctg gcgacgctgc ggacggtgga gaacggatcg ggtgcgaacg gcataggtca
     3181 gcgagccagc cagtcgagca gcatgcgctg cacgtggaaa cggttctcgg cctcgtcgat
     3241 cacccggctt gccggtgagt cgagcacggc gtcgcccacc tcgacgttgc ggcgcacggg
     3301 caggcagtgg aggaagattg catcgctcgc ggcgcgggcc agctgccgcg cctcgatgcg
     3361 ccaggagggg aacgcgcgaa ccgcctcggg tccgagcggc ccgggagcgg ccggtcccca
     3421 ggccttggcg tacacgaccc gcgagccctc gagcgcctcg tcctggtccg aggtgaacgt
     3481 cacctcgccc cccgaggcgc gcgcgaactc ccttgcctcg gagacgacgg ccgggtgcag
     3541 ttcgaagccg ggcgggtgcg ccacgcgtac gcggcagccg gccgcgccgg cggtcaacag
     3601 gaacgagtgc ggcaccgcct tcggcagcgg cttcacgtgc ggtgcccagg tcagcgtcac
     3661 cgggagtccc ctcgtctcgc cgaaggtttc cgcgatggtc agcacgtcgg ccaacccctg
     3721 gcacgggtgc tcgcgcgcgc tctccatgct gatgacgggc acggtggagt agtcggcaaa
     3781 ggcgcgcatc accgggtcgc gctcatcgtc atcgtcattc gtgcaggagg cgaaggtgcg
     3841 caccgccagt aggtccacgt agcggcccag caccggcacc gcctcgcgta cgtgctcggc
     3901 gcggtcctgg tccatgaccg ccccgatccg gtcctcgagc ttccacacgc cgcccccgac
     3961 ttcgaggacg atcgcgtgcc cgccgccccg cgccatgacg gcctcgaagc tggcccgcgt
     4021 ccggaggctc gggttgaaga acaccatgcc gaggatcttg ccggccaggt gcgcctcggg
     4081 gggacagcgc ttccaggcga tcgcctggtc gagaacggcg tgcactccat cgggaccgag
     4141 atcggcgaga caagtgaaat gacgcatggc ttcagcgctc cgccgcgaac gaggcaatgg
     4201 cctcgatcag ggcatcgatc gacgagtcgc gaacggtgag cggcggcatc aggcgcagca
     4261 cgtcgggatc ggccgacccg cccacgagaa tccgcttgtc ctggagaaac gtcttcagct
     4321 cgccggcgcg gccgggcacc cggaggccaa gcagcaggcc gcggccgtga accgcggtga
     4381 ccgcggtccc ggcgacgcca gcgcgaatgc gctcgccgca caccctggcc ctctccatga
     4441 ggc
//
LOCUS       DAOPQX010000318         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21333_length_4441_cov_5.373689, whole genome shotgun sequence.
ACCESSION   DAOPQX010000318 DAOPQX010000000
VERSION     DAOPQX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21333_length_4441_cov_5.373689"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKK95_14130"
     CDS             <1..219
                     /locus_tag="PKK95_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19407.1"
                     /translation="TVATTTQAAPVWLRLKRGSGGITASVSTNGSSWRTVGTVSLGMT
                     SKSVVGLVVASGSTSARATATFDNVRVP"
     gene            471..>2024
                     /locus_tag="PKK95_14135"
     CDS             471..>2024
                     /locus_tag="PKK95_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOC19408.1"
                     /translation="MSQTPAKSLVAMIVFASVGLAASPPDPPQLGKSPLREVIAAMTP
                     EEKAAIVVGTGMNLPPGMSAGPGMQAPVVGETIKGVPGAAGTTFPIARLGIPAVILAD
                     GPAGLRIQPKRPGEESRTYYCTAFPIATLLASSWDVELLERVGRAMGSEVREYGVDVL
                     LGPALNIHRNPLGGRNFEYYSEDPLLSGKLAAAMTRGVQSQGVGTSAKHFVANDHEWN
                     RNVIDVKVSQRALREIYLRPFEILVREAQPWTVMSSYNRLNGTYTSESAPLLNGVLRE
                     DWGFEGLVMTDWFGGRDPVAQMKAGNDLLEPGTAAQQQAVLAALKSGALPPEVLDRNV
                     ERVLSLVLRSPTFKNATRSNQPDLAANAAVGRDAAAQGMVLLKNEGALPLPRPAKTIA
                     LFGNTSYDMITGGSGSGDVNEAYTVSLVQGLEAAGLAAEAKLAAAYSSHLQEFARTAP
                     PPRMFMAAPRAAEMSLAPADIARAAKESDAGLVTIGRNAGEGRDREVAGDFELTAVEK
                     ALIAEVASAF"
     assembly_gap    2025..2034
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2035..2801
                     /locus_tag="PKK95_14140"
     CDS             <2035..2801
                     /locus_tag="PKK95_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HOC19409.1"
                     /translation="ANGKKALVVLNVAGVVETASWRDLPDAILLAWQPGQESGHAIAD
                     VITGKVTPSGKLATTFPMKYQDVPSASNFPGKTLVGPDPAARGGNVFGRGDREAEIAY
                     EDDVLVGYRHYATKDVKVAYPFGFGLSYTAFEYSGLSLSGPEFGTGLTVKVTVRNTGK
                     VAGREAAQLYLSAPGKTMLKPAIELKGFAKTKVLAPGESQTLAFQLTPRDLASFDEAT
                     SSWQAEAGTYTVRIGASSADIRQTATFTKATTERVE"
     gene            2874..3470
                     /locus_tag="PKK95_14145"
     CDS             2874..3470
                     /locus_tag="PKK95_14145"
                     /inference="COORDINATES: protein motif:HMM:NF025432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOC19410.1"
                     /translation="MPAREKAVGGADRVRGDLSAGQRKDLLETLRARFEENMNRHKGV
                     EWARVQARLEAHPGALWSLSQMEKTGGEPDVVGLDRATGECVFFDCSPESPKGRRSLC
                     YDREALEARKENKPGDSAIHMAAAMGIEILSEEQYRELQALGEFDTKTSSWVKTPPEV
                     RKLGGAIFCDRRYGRVFTYHNGAESYYAARGFRAWLKV"
     gene            4042..4275
                     /locus_tag="PKK95_14150"
     CDS             4042..4275
                     /locus_tag="PKK95_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19411.1"
                     /translation="MIDTRMFVVELRGPSPDTTVRTVALRGGAFDFGSVPNGRYELKT
                     TASGSAFALGWQSEIRTVIVSDSADPGAEIRIP"
BASE COUNT          711 a         1476 c         1566 g          678 t
ORIGIN      
        1 acggtggcca ccaccacgca ggcggcgccg gtctggctgc ggctcaagcg gggcagcggc
       61 ggcatcaccg cctcggtttc gacgaacggc tcgtcgtggc gcacggtggg gaccgtcagc
      121 ctgggcatga cgtcgaagtc ggtcgtcggc ctggtggtcg cgagcggcag cacgtcggcc
      181 cgcgccaccg ccaccttcga caacgtgcgc gtgccgtaag cggataggtc tttcgtttcg
      241 tcagcgattc ggagggggag acgggccgcg tcccgtctcc ccttggtttc tccagcctct
      301 ccccaccgag ccgtaccggc cgccctccct cgcccagctc gcggcactcc atcttccggg
      361 aactcgcccg cagggaccgc gatcagccag ctcttgcgtt tcccggccgt tcgccgccat
      421 gattgcgccc tctgctgtga accggcagcc ggacgagtgg gagggtttcg atgtcacaaa
      481 cgcctgcaaa gagcctggta gccatgatcg tcttcgcctc ggtgggcctc gccgcgtcgc
      541 cgcccgaccc gccgcagctc gggaagagtc cgctccgcga ggtcattgcc gccatgaccc
      601 cggaggagaa ggccgcgatc gtcgtcggaa ccggcatgaa cctgccgccg gggatgagcg
      661 ccggcccggg catgcaggct cccgtggttg gcgagacgat caagggggtt ccgggcgcgg
      721 cgggcacgac ctttccgatc gcgcgcctgg gcatcccggc cgtgatcctc gccgacgggc
      781 ccgcgggcct gcgcatccag ccgaagcgcc ccggcgagga gtcgcgcacc tactactgca
      841 cggcgtttcc gattgcgacc ctgctcgcct cgtcgtggga cgtggagctg ctggagcggg
      901 tgggccgcgc gatgggcagc gaggtccgcg agtacggcgt ggacgtgctg ctcggccccg
      961 cgctcaacat ccaccgcaac ccgctcggcg gccgcaactt cgaatactac tccgaggatc
     1021 cgctgctgtc gggcaaactg gccgcggcga tgacgagggg cgtccagtcg cagggcgtgg
     1081 ggacctcggc caagcacttc gtcgccaacg accacgagtg gaaccgcaac gtcatcgacg
     1141 tgaaggtgtc gcagcgggcg ctgcgcgaga tctacctgcg ccccttcgag atcctcgtgc
     1201 gcgaggcgca gccgtggacg gtgatgtcct cctacaacag gctgaacggc acctacacct
     1261 ccgagagcgc gccgctgctg aacggcgtgc tgcgcgagga ctggggcttc gagggcctgg
     1321 tgatgaccga ctggttcggc ggccgggacc ccgtcgcgca gatgaaggcg ggcaacgacc
     1381 tcctcgagcc cggcaccgcc gcgcagcagc aggcggtgct ggccgcgttg aagtcgggcg
     1441 cgctcccgcc cgaggtgctc gatcgcaacg tcgagcgcgt gctgagcctc gtgctccgca
     1501 gccccacgtt caagaacgcg acccggtcca accagcccga cctcgccgcc aacgcggcag
     1561 tcgggcgcga cgcggcggcc cagggaatgg tgttgctgaa gaacgagggg gcgctgccgc
     1621 tcccccggcc ggcgaagacg atcgcgctgt tcggaaacac gtcctacgac atgatcacgg
     1681 gcggctcggg ctcgggcgac gtgaacgagg cgtacacggt ttcgctggtg caggggctcg
     1741 aggcggccgg cctcgccgcc gaggcgaagc ttgcggccgc ctattcgtcg cacctgcagg
     1801 agttcgccag gacggcgccg ccgccgagga tgttcatggc ggcgccgcgc gcggctgaga
     1861 tgtccctggc ccccgcggac atcgcgaggg ccgcgaagga gagcgacgcg ggcctcgtca
     1921 cgatcggccg gaacgccggc gaggggcgcg accgcgaggt ggcgggcgac ttcgagctga
     1981 ccgccgtgga gaaggctttg atcgcggagg tggcctcggc cttcnnnnnn nnnnacgcga
     2041 acggcaagaa ggccctcgtc gtcctcaacg tcgcgggggt cgtcgagacg gccagctggc
     2101 gcgatctgcc agacgcgatc ctgctggcgt ggcagcccgg ccaggagtcg ggtcacgcca
     2161 tcgccgacgt gatcaccggg aaggtgacgc cgtcgggcaa gctggcaacg accttcccga
     2221 tgaagtacca ggacgtgccg tcggcgtcga actttccggg caagacgctc gtcggccccg
     2281 acccggcggc caggggcggc aacgtctttg ggcgcggcga ccgcgaggcg gagatcgcct
     2341 acgaagacga cgtcttggtc ggctaccggc actacgcgac caaggatgta aaggtggcct
     2401 acccgttcgg gttcggcctg tcgtacacgg cgttcgagta ctcgggcctt tcgttgagcg
     2461 gccccgagtt cggcaccggg ctgacggtga aggtcacagt ccggaacacc gggaaggttg
     2521 cgggtcgcga agcggcgcag ctgtatcttt cggcgccggg caagacgatg ctcaagccgg
     2581 caatcgagct gaagggcttc gccaagacga aggtgctggc gccgggggag tcgcagacgc
     2641 tcgccttcca gctgaccccg cgtgacctgg cgtcgttcga cgaggcgacc tcgtcatggc
     2701 aggcagaggc cggtacctac accgtcagga tcggcgcctc gtctgcagat atccggcaga
     2761 ccgccacctt cacgaaggcc acgaccgagc gggtggagta gttccccgca gagcttgccg
     2821 ggccctcgtc accggtcgca ccatgggctg tgcgataaag gggtgaggtg gcgatgccgg
     2881 cacgtgaaaa ggcagtaggg ggagcggacc gtgtccgggg tgatctttcc gccgggcagc
     2941 gcaaggatct tctcgagacg ttgagggccc ggttcgagga gaacatgaac cggcacaagg
     3001 gcgtcgagtg ggcgagggtg caggcgagac tggaggccca ccccggagcg ctgtggtctc
     3061 tcagccagat ggagaagacc gggggcgaac cggacgtggt gggcctcgac cgcgcgaccg
     3121 gcgagtgcgt cttcttcgac tgttcgcccg agagccccaa gggacgccgc agcctctgtt
     3181 acgaccgcga agcgctggag gcgcggaagg agaacaagcc gggggacagc gcgatccaca
     3241 tggccgccgc catggggatc gagatcctgt cggaggagca gtaccgcgag ctgcaggccc
     3301 tcggcgagtt cgacacgaag acgtcgagct gggtgaagac gccgccggag gtcaggaaac
     3361 tggggggcgc catcttctgc gaccgccgct acggccgcgt cttcacgtac cacaacggcg
     3421 ccgagtccta ctacgcggcg aggggcttcc gcgcctggct caaggtgtga ggcggtcctt
     3481 gccagttgcc acagccagtg gcacacccag tggcacaggc gtcccgcctg tgtgagggtc
     3541 tccctcacca gtggcacagg cgtcccgcct gtgaagggat atcctgccag tggcacaggc
     3601 gtctcgcctg tgaagggata tcctgccagt ggcacaggcg tcccgcctgt ggctcgggtg
     3661 aagaacccgc cactgcttca ttctcgccgc gcgagctttc cggccctcct cggctacaat
     3721 gcgcaccaga ggccgggagc aggaaatatg cgcattcgcg gtctcggcgc ctttctcgcg
     3781 atcctcctta ctaccgcctg tgcatccgtc cagcgtgtgc cagttccgca ctggggccgc
     3841 tacgaccagg gcgagctttc cggtttcctt cgcgccccag gcgaacagct tgtgaggagg
     3901 gtaccggaac tgatagtgca gcgggctcgg gggaggattg ccttgccaac cgccgagggg
     3961 cggcgcgagc taccacttca gtcgtggcca acgactccgg gtgaggccgt gagtgcgcca
     4021 taccgtcccc ccgaatatgc ggtgattgac accaggatgt tcgtggtcga actacgcggt
     4081 cccagccccg acacgaccgt gcgcacggtc gcgctgcgag gcggcgcgtt cgacttcggg
     4141 tccgtgccca acggcaggta cgagctgaag acgacggcct cagggtcggc gttcgcgctt
     4201 ggatggcagt ccgagatcag gaccgtcatc gtgtccgatt ctgccgatcc gggtgcagag
     4261 atccggattc cctgagtcgt cacctcgccg gcatcgcaca tccgtcgtac caacaggcga
     4321 ctccgggtct cgcccgccgc ttcagggtca accggccgac acgccaagac tttgaattag
     4381 gaggactcgc gggttggtcg gggcgccggg attcggaccc gggacctcct gcgcccaagg
     4441 c
//
LOCUS       DAOPQX010000319         4438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21358_length_4438_cov_5.080082, whole genome shotgun sequence.
ACCESSION   DAOPQX010000319 DAOPQX010000000
VERSION     DAOPQX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21358_length_4438_cov_5.080082"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKK95_14155"
     CDS             complement(<1..1144)
                     /locus_tag="PKK95_14155"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOC19412.1"
                     /translation="MPSFVLFAIAATVAAGWSGDFTERYRLALERVLQGGPPRYTVEF
                     VAADAVPRHVRRFTEFSGDVSGRYLEALAVSARERPDLQPAVNAVADAVIPHQQPDGH
                     FGEAFSEGRITQKHMAMLWGNGRLLVGLVEAHRLTRRADLLEAARRLGGFFLAVAPRL
                     NDDGVRREYSGTQVAVGYICWTQVIEGLVALHEETRDARYLELGRAIAAHTGRHPAQH
                     SHGYLTSLRGILQLHRATGDPALLAQVEREWDELMASGNVFVHGAVPEAFKPIAKRDE
                     GCSEADWLRLNLALWRATGKAKYLEQAELTLFNEFAFNQFGSGDFGHRELTDEGVAAW
                     GARAWWCCTFHGLRAFPDLFAAAFREDAQGLHYDLAIPGRGQAGGLT"
     gene            complement(1264..2835)
                     /locus_tag="PKK95_14160"
     CDS             complement(1264..2835)
                     /locus_tag="PKK95_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOC19413.1"
                     /translation="MDAVVAGTVPAGGEASENSHASAASRRFVRGSSLLLAGRFISVL
                     LNFAVQVLAVRYLSKGHYGAFAYGIGVATLGSSILLLGLAKGIPRLVPIYSERNEQDK
                     AFGAIVLSVATICVLGLGLVLGLFVLQDQVGSGANLDRETLSLLLVLIAIAPIDALDK
                     LLQQVVAVYCSARAIFFRRQVVGPGLKLAAVLFVMAVTGDVYMLAYGYVLGSLIGVSL
                     YLATLLRTWSRQGLLQYLRPGKYRLPFRELFGFSIPLLTTDLSVALRGALVVILLEYF
                     RDASSVGEYRAVVSVAHLNTVVFEAFGFLFVPLASRMYARNDRQGIGDLYWATSLWIA
                     VLTFPLFAATCVLSPTITVLLFGDRYAGAAVLLSVLAAGHYFNAALGFNASTLRVHGK
                     VRYIVAADVLTVVLCVALGVVLVGRYGALGGALTASLALILNNLFVHAGLWMGRTGVP
                     LFPWKIARVYLLITLATLGLAAGDRILLLPAWMTAVLAGAVGVAVLRVSRGVLNPGAN
                     FPEMLRVPVLRRLLA"
     gene            3117..>4438
                     /locus_tag="PKK95_14165"
     CDS             3117..>4438
                     /locus_tag="PKK95_14165"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOC19414.1"
                     /translation="MSAHNRRQFLGVTAASFAAASLFPRRVAGRSPADVPALLGGTPV
                     HKGAWPKWPEWRQAWEPSILDVLRSGQWYRGASGDQIPRFEAAYAKLLGAKRCLATAS
                     GTTALLVAMHVLDVDAGDEVIVSPFTFIASYNAILMLKALPVLADTDPATLTIDPASI
                     ETRITDRTRAIMPVHIYGMPCDMDPIVALAAKHRVRVVEDACQAWLAEYKGRKCGTIG
                     DLGCFSFQNSKHLPSGEGGAITGNSDDLLDRASSFHDCGRPYGGFKGARPNFTRGGNF
                     RIQHFQAAMLLQQLDKLVTDTARRREHASRLTSALQDIPGIRTVRLPQNSTAVWHLYP
                     FRYDAEKFEGLSRATFIKAMRAEGIPCSTGYQEQYFDGLLDETIASRGFKRLFSERRL
                     KEYRDSFNELKGNREVCATTVAIGQNMLLAERAEIDQIAAAVQKIQANA"
BASE COUNT          787 a         1577 c         1503 g          571 t
ORIGIN      
        1 cggtgagccc gcccgcctgt cccctccccg ggatcgccag gtcgtagtga aggccctgcg
       61 cgtcctcgcg gaaggcggcg gcgaacaggt cggggaacgc gcgcaagccg tggaaggtgc
      121 agcaccacca ggcgcgcgcc ccccaggccg ccaccccttc atcggtcaac tcgcggtgcc
      181 cgaagtcgcc gctgccgaac tggttgaacg cgaactcgtt gaagagcgtc agctcggcct
      241 gctccaggta cttcgccttg ccggtcgcac gccacagggc caggttgagt ctcagccagt
      301 cggcctccga gcagccctcg tctcgcttcg cgatcggctt gaacgcctcg ggcacggcgc
      361 cgtgcacgaa gacgtttccc gacgccatca gctcgtccca ctcgcgctcc acctgcgcca
      421 gcagcgccgg gtcgccggtg gcgcgatgca gctgcaggat cccgcggagc gaggtcaggt
      481 agccgtggct gtgctgcgcg ggatggcgcc cggtgtgggc ggcgattgcc cgcccgagct
      541 cgaggtaccg cgcgtcgcgc gtctcctcgt ggagcgcgac caacccctcg atgacctgcg
      601 tccagcagat gtaccccaca gccacctgcg tcccgctgta ctcccggcgc accccatcgt
      661 cgttcaggcg cggcgcgacc gcgaggaaga agccgcccag ccggcgggct gcctcgagga
      721 ggtcggcgcg cctcgtgagc cggtgcgctt ccaccaggcc gacgagcagc cgcccgttgc
      781 cccagagcat cgccatgtgc ttctgggtga tccgtccttc ggagaatgcc tcgccgaagt
      841 ggccgtcggg ctgctggtgt gggatcacgg cgtcggcaac cgcgttcacc gcgggctgca
      901 gatccgggcg ctcgcgcgcg ctcaccgcca gcgcctcgag gtagcgcccc gacacgtcgc
      961 cgctgaactc ggtgaaccgc cggacgtgcc gcggcaccgc gtcggcggcg acgaactcca
     1021 ccgtgtagcg gggcgggccg ccctggagca cgcgctcgag cgccagtcgg tagcgctcgg
     1081 tgaaatcacc ggaccatcca gccgcgacgg tcgcggcgat cgcgaagagg acgaagctcg
     1141 gcatgtggcc cccgtggctg ggcgaggcgg cgcctcgccc tccgtttcgc cgatcctagc
     1201 cgatctcgaa ccggcgtcgc ggacgcaacc agatgggtga ccgggaaggc cgggaagtga
     1261 aggtcaggcc agcagccggc ggagaacggg aacccgcagc atttcgggga agttcgcacc
     1321 cgggttgaga acgccgcgcg agacgcgcag gacggccacg ccgaccgccc cggcgaggac
     1381 ggcggtcatc caggcaggaa gcagcaggat ccggtcgccg gccgcgagcc ccaaggtggc
     1441 gagcgtgatc aggaggtaca cgcgggcgat cttccaggga aagagcggga cgcccgtgcg
     1501 ccccatccac aggccggcgt gcacgaacag gttgttgagg atcagcgcca gcgacgcggt
     1561 gagcgcgccg ccaagggcgc cgtaccgccc gaccagcacg acgccgaggg ccacgcacag
     1621 cacgacggtc agcacgtcgg ccgcgacgat gtaccggacc ttcccgtgaa cccggagggt
     1681 ggaggcattg aacccgagcg ccgcgttgaa gtagtggcca gccgccagga cgctgagcag
     1741 gaccgccgcg ccggcatatc ggtcgccgaa gagcagcacc gtgatggtgg gcgacaggac
     1801 gcacgtggcc gcgaacaggg ggaacgtcag gaccgcgatc cagagtgacg tcgcccagta
     1861 caggtcgccg atcccctgcc tgtcgttcct ggcgtacatg cgggacgcca gcggaacgaa
     1921 caggaaccca aatgcctcga acaccacggt gttcaggtgg gcgaccgaga cgactgcacg
     1981 gtattccccc accgacgagg cgtcgcggaa gtactccagc aggatcacga ccagcgcccc
     2041 tcgcagggcg acggagaggt ccgtggtgag aaggggaatg ctgaacccga acagctcgcg
     2101 gaacggcagg cgatacttcc cgggcctcag gtattgaagc aggccctgcc tgctccaggt
     2161 ccgaagcagg gtggcgagat agagcgaaac gccgatgagc gatccgagaa cgtagccgta
     2221 cgccagcatg tacacgtcgc cggtgaccgc catgacgaag agcaccgcgg ccagcttgag
     2281 gcccggtcca acgacctgcc gccgaaagaa gatcgctcgc gccgagcagt agaccgcgac
     2341 gacctgttgc agcagcttgt cgagcgcatc gatcggggcg attgcgatga ggaccagcag
     2401 cagcgacagc gtctcgcggt ccaggttcgc gccgctcccc acctggtcct ggagcacgaa
     2461 cagcccgaga acgagcccaa ggccgagtac gcagatcgtc gccacggaga ggacgatggc
     2521 cccgaaggcc ttgtcctgct cgttccgctc gctgtagatc ggcacgaggc ggggaatccc
     2581 cttggcgaga cccagcagca ggatgctcga accgagggtg gccaccccga tcccgtacgc
     2641 gaaggccccg tagtgcccct tcgacaggta ccgcacggcg agcacctgca ccgcgaagtt
     2701 gaggagcacc gagatgaacc gacctgccag cagcaggctg gagccgcgga cgaaccgccg
     2761 actcgccgcc gaagcgtggc tgttctcgct cgcttcccca ccggccggga cggtcccggc
     2821 aaccacagcg tccactccaa ctccttcaat cgccgtttgc cggcggccga cggccgcagc
     2881 acatccggcg tcacgccaca ccgcgagcag cgagggtcca ggctggcact ctcgccgcca
     2941 cagccggcac aaccatcacg ttcattctga ccaccagcag ggcgagggaa catgggagca
     3001 gggtcccagc acgggcagga atcgccaggc gcggcctcct ggcgacgcgc gcgccaggat
     3061 ctgcactaca cttcccggca cggacgggat cgcgcgactg aacaggagga cgtcccatga
     3121 gcgcccacaa ccgccgccag ttcctgggtg tcaccgcggc aagcttcgcc gccgcaagcc
     3181 tcttcccgcg gcgagtcgcc ggccgctcac ccgccgacgt cccggcgctg ctgggaggca
     3241 cccccgtcca caagggcgcc tggccgaagt ggccggagtg gcgccaggcg tgggagccgt
     3301 cgatcctcga cgtcctgcgc agcggccagt ggtaccgggg cgcctccggc gaccagattc
     3361 cccgcttcga ggccgcctac gcgaagctcc tcggcgcgaa gcgctgcctg gcaacggcca
     3421 gcggcacgac cgccctcctc gtggcgatgc acgtcctcga cgttgacgcc ggcgacgagg
     3481 tcatcgtctc gccgttcacc ttcatcgcca gctacaacgc catcctgatg ctgaaggcgc
     3541 tgccggttct cgccgacacc gacccggcga cgctgacgat cgacccggcc tcgatcgaga
     3601 cccggatcac ggaccggacg cgcgcgatca tgccggtgca catctacggc atgccgtgcg
     3661 acatggaccc gatcgtggcg ctggccgcga agcaccgggt gagggtcgtc gaggacgcct
     3721 gccaggcctg gctggcggaa tacaagggcc gcaagtgcgg gacgatcggc gacctcggct
     3781 gcttcagctt ccagaactcg aagcacctgc cgtcgggcga gggcggggcg atcaccggca
     3841 acagcgacga cctgctcgat cgcgccagct cgttccacga ttgcgggcgc ccgtacggcg
     3901 gcttcaaggg ggcgcggccg aacttcaccc gcggcggcaa cttccggata cagcacttcc
     3961 aggcggccat gctgctgcag caactcgaca agctggtgac cgacaccgcc cggcgccgcg
     4021 agcacgcgag ccgcctgaca tccgcgctcc aggacatccc aggcatccgg accgtgcgcc
     4081 tgccccagaa cagcacggcg gtctggcacc tctacccctt ccggtacgac gccgagaagt
     4141 tcgaggggct ttcacgggcg accttcatca aggcgatgcg cgccgagggc atcccctgct
     4201 ccacgggcta ccaggagcag tacttcgacg ggctgctcga cgagacgatc gcctcgcggg
     4261 gcttcaagcg gctgttcagc gagcggcggc tgaaggagta ccgcgacagc ttcaacgagc
     4321 tgaaaggcaa ccgcgaggtc tgcgcgacga cggtggccat cggccagaac atgctgctgg
     4381 ccgagcgggc cgagatcgat cagattgccg cggcggtgca gaagatccag gccaacgc
//
LOCUS       DAOPQX010000320         4436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21370_length_4436_cov_2.683406, whole genome shotgun sequence.
ACCESSION   DAOPQX010000320 DAOPQX010000000
VERSION     DAOPQX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21370_length_4436_cov_2.683406"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKK95_14170"
     CDS             <1..296
                     /locus_tag="PKK95_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19415.1"
                     /translation="ALGLAVYDTQCGAKLFRTSEVVAAAFARPFRTGWVFDVELLARY
                     VAAVGHDEARARIYELPLRKWIEVPGSKVRPWHGPGAVLDLLWIAWALRRGDV"
     gene            complement(327..1682)
                     /locus_tag="PKK95_14175"
     CDS             complement(327..1682)
                     /locus_tag="PKK95_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOC19416.1"
                     /translation="MTIRLTDLGRAVLQTEYAVRGPIVARAQALEREGREIIYCNIGN
                     PQALEQKPITFLRQVLALCQYPDLMTRGGDLFPRDVIATARKILAGTRHGLGAYSDSK
                     GVRFIREAVAAFIHERDGIPADPEAIYLTDGASKGVQTALRLLISGPGDGIMIPIPQY
                     PLYSATITLYEGRQVGYYLDEGNDWRLSRLRLEESYEKATAEGVRVRAICVINPGNPT
                     GAVLDCANISMIVDFARERGLAILADEVYQENIYLAGDRFHSFARVVTEKGEREVSLF
                     SFHSCSKGFLGECGQRGGYMELRNVPAEVVAQVTKLQSVALCANLTGQVATYCMVHPP
                     RSGDESYPLYASERDRIIGDLRRRAILLAEGLNRIPGIRCNQVAGAMYAFPSITLPPG
                     RTDEEYCLALLEETGICVVPGSGFGQVPGTAHLRTTILPPTEKIEALVQRLARFHASY
                     R"
     gene            complement(1766..2503)
                     /locus_tag="PKK95_14180"
     CDS             complement(1766..2503)
                     /locus_tag="PKK95_14180"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOC19417.1"
                     /translation="MRGVPLLLGLLLLGGCARAGDSAGERAHGGGDEAARERMVSRQL
                     EGRDITDRKVLEAMRAVPRHLFVPPDLRDRAYDDTPLPIGHGQTISQPYIVALMTQLV
                     RPGPADRALEIGTGSGYQAAILARLVQEVYSIEIVEPLAREAEERLRELGFGNVTVRA
                     GDGYRGWPERAPFDIVMVTAAPDHLPPALLEQLGPGGRLVIPIGPRGGGQVLRLFEKD
                     REGRVASRDVAPVLFVPLVRAGAPAGR"
     gene            2597..>4436
                     /locus_tag="PKK95_14185"
     CDS             2597..>4436
                     /locus_tag="PKK95_14185"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOC19418.1"
                     /translation="MHLPLPCRSVVFTCVLAAALLANLPASAAAGAAQQPGAKPSPAP
                     PALHVGRVAAPPSLDDFIAGRPTPGMTELSGFRQREPGDGTPVSSPTTAWVGYDERQL
                     HVVFVCGEKPGQVRGRLARREDINEDDVVGVLLDTFHDRRHAYLFIVNPLGVQLDGIS
                     TEGQDDDYSFDTLWHSEGRLTPGGYVARVSIPFRSLRFANTPTQEWGFALGRVIRSRN
                     ETSFWPYVTRRVQNHIEQMATLTGIEGVSPGRNIQLIPYTAGTASRFLDEEAAERGSR
                     EEARAGLDAKVVIKDALTLDLAANPDFSQVESDEPQVTINQRFEVQFPEKRPFFIENA
                     SYFRTPETLFFSRRLADPRLGARLTGRVGKWNVAALVMDDRAPGEQAPRGSEASGAMA
                     ATAVGRVVREFGRQSTVGAMATSRDFSTSSNRVGSVDLRLRLGGNWVIDAQAMTSRTT
                     RLDGERLAGPAYVAQVGYYGRSLNFVTEYTDRSPGFRADLGFIQRSDIREWETEAGYK
                     WFPKGGTLLSWGPGFEATVNWDRTGRVQDWRVNPEVELEFPRNTNLFAGVDWAYERFA
                     GIGFDKRAAFGFFNTEWLKWLSISLGLSRSRGVNYYPAQGLTPFLAN"
BASE COUNT          659 a         1548 c         1579 g          650 t
ORIGIN      
        1 tcgccctcgg gcttgccgtc tacgacacgc agtgcggggc aaagctgttt cggacgagcg
       61 aggtcgtcgc cgccgccttt gcgaggccgt tccgcaccgg ctgggtgttc gacgtggagc
      121 tgctggcccg gtacgtcgcg gcggtgggcc acgacgaggc cagggcgcgg atctacgagc
      181 tgccgctgag gaagtggatc gaggtgcccg gatccaaggt caggccgtgg cacggccccg
      241 gagccgtgct cgaccttctg tggattgcct gggcgctccg ccggggagac gtctgacggg
      301 aggtgaaggg acgatccccg agccttctac cggtagctcg cgtggaaccg cgcgagccgc
      361 tgcaccaacg cctcgatctt ctcggtgggc ggcaggatgg tcgtgcgcag gtgcgcggtg
      421 ccgggaacct ggccgaagcc ggagccgggc accacgcaga tgcccgtctc ctcgagcagc
      481 gccaggcagt actcctcgtc ggtgcggccg ggcggcagcg tgatgctggg gaaggcgtac
      541 atcgcgcccg ccacctggtt gcaccggatg cccgggatgc ggttcaaacc ctcggccagc
      601 aggatcgcgc ggcgccgcaa gtcgccgatg atgcgatcgc gctcgctcgc gtagagcggg
      661 tacgactcgt cgccggacct gggcgggtgc accatgcagt aggtggccac ctggcccgtg
      721 aggttcgcgc aaagcgccac cgactggagc ttggtcacct gcgccaccac ctcggccggg
      781 acgttccgca gctccatgta gccgccgcgc tggccgcact cgccaaggaa ccccttggag
      841 caggagtgga agctgaagag gctgacctcg cgctccccct tctcggtcac gacgcgcgcg
      901 aacgagtgga accggtcgcc cgccaggtag atgttctcct ggtacacctc gtcggccagg
      961 atcgcgaggc cgcgctcgcg cgcgaaatcg acgatcatcg agatgttcgc gcagtcgagg
     1021 acggcgccgg tcgggttgcc cgggttgatc acgcagatcg cgcgcacacg cacgccctcg
     1081 gcggtcgcct tttcgtagct ctcctcgaga cggaggcggc tgagcctcca gtcatttccc
     1141 tcgtccaggt agtagcccac ctggcgcccc tcgtacagcg tgatggtcgc gctgtacaac
     1201 gggtactgcg gaatcgggat catgatcccg tcgccgggcc cgctgatcag caggcgcagg
     1261 gctgtctgaa cccccttcga ggcgccgtcg gtgaggtaga tcgcctccgg gtcggccggg
     1321 atcccgtcgc gctcgtggat gaaggcggcc accgcctcgc ggatgaaccg gacgcccttg
     1381 ctgtcggaat acgccccgag gccatgccgc gtgcccgcca ggatcttccg cgcggttgcg
     1441 atcacgtcgc gcgggaacag gtcgcccccc cgcgtcatca ggtccggata ctggcagagc
     1501 gcgagcacct gccgcaggaa ggtgatgggc ttctgctcga gggcctgcgg gtttccgatg
     1561 ttgcagtaga tgatctcgcg cccctcgcgc tcgagcgcct gcgcgcgggc gacgatcggg
     1621 cctcggacgg cgtattcagt ctgcaggacg gccctgccga gatcggtcag gcggatggtc
     1681 atgatggtcc gttcttcgca agggcgcgct cgggagcggc ggcgatggcc ccgaggagcg
     1741 attctgtcgg aaagggggcc ggaattcaac gcccggccgg cgcacccgcg cgcacgagcg
     1801 gcacgaagag caccggcgcc acgtcgcgtg acgcaacgcg cccctcgcgg tccttctcga
     1861 agagccgcag cacctggccg cctccccgcg gcccgatcgg gatcaccagc cgcccccccg
     1921 ggccgagctg ctcgagcagc gccggcggca ggtggtcggg ggcggccgtc accatgacga
     1981 tgtcgaaggg agcgcgttcg ggccagccgc ggtagccgtc ccccgcgcga accgtgacgt
     2041 tgccgaaccc cagctcccgc aggcgctcct cggcctcgcg tgcgaggggt tcgacgatct
     2101 cgatcgagta gacctcctgc acgagcctgg cgaggattgc cgcctggtag cccgacccgg
     2161 ttccgatctc gagcgcgcga tcggccggcc cgggccggac cagctgcgtc atgagcgcga
     2221 cgatgtaggg ctgcgagatc gtctgcccgt ggccgatcgg caacggcgtg tcgtcgtacg
     2281 cgcggtcgcg gagatcgggc gggacgaaca ggtgccgagg gacggctcgc atcgcctcga
     2341 gtaccttccg gtcggtgatg tcccggccct ccagctgacg agacaccatc cgttcgcgcg
     2401 ccgcctcgtc cccgccgccg tgcgctcgtt cgccggccga atcgccggcc cgggcgcagc
     2461 ctccgaggag cagcaagccg aggagcagcg gcaccccgcg catgccccga gtataggacg
     2521 ccggaaccgg ccgggcacgt tttcgtctat gctgacgatc cgcccgcgac cgaccgtccc
     2581 ccgggatcca gccgagatgc accttccgtt gccctgccgt tccgtcgtgt tcacctgcgt
     2641 gctcgccgcc gcgctgctcg cgaacctgcc cgcgtccgct gcagccggcg ccgcccagca
     2701 gccgggtgcc aagcccagcc cggcgccgcc ggccctgcac gtcgggcgcg tggcggcccc
     2761 gccgtcgctc gacgacttca tcgcaggccg gccgacgccg gggatgaccg agctcagcgg
     2821 cttccggcag cgcgagccgg gtgatggcac gccggtcagc agccccacga ccgcctgggt
     2881 ggggtacgac gaacggcagc ttcacgtcgt gttcgtctgc ggggagaaac cgggacaggt
     2941 gcgcggccgg ctcgcacgcc gcgaggacat caacgaggac gacgtcgtcg gggtcctgct
     3001 cgacacgttc cacgacaggc gccacgccta cctcttcatc gtgaacccgc tcggcgttca
     3061 gctcgacggc atcagcaccg aggggcagga cgacgactac tcgttcgaca cgctgtggca
     3121 cagcgagggg cgcctgacac cgggcgggta cgtcgcgcgc gtcagcatcc ccttccgcag
     3181 cctccgcttc gccaacaccc cgacgcagga gtggggcttc gccctcgggc gggtgatcag
     3241 atcgcgcaac gagacgtcgt tctggcccta cgtcacgcgg agggtgcaga accacatcga
     3301 gcagatggcg acgctgacgg gaatcgaggg cgtgtccccg ggacgcaaca tccagctgat
     3361 tccgtacacg gcggggacgg cgtcgcggtt cctcgacgag gaggcggccg agcgcggcag
     3421 ccgcgaggag gcgcgggcgg gcctcgacgc gaaggtggtg atcaaggacg cgctgacgct
     3481 cgacctggcg gccaacccgg acttcagcca ggtcgagtcg gacgagccgc aggtgacgat
     3541 caaccagcgc ttcgaggtgc agttccccga gaagcggccg ttcttcatcg aaaacgccag
     3601 ctacttccgc acgcccgaga ctctgttctt ctcgcggcgc ctggcggacc cccgcctggg
     3661 cgcgcggctg acggggcggg tcgggaagtg gaacgtcgcc gcgctggtga tggacgaccg
     3721 ggcgcccggc gagcaggcgc cccgcggaag cgaggccagc ggcgcgatgg ccgccaccgc
     3781 cgtcggccgc gtggtgcgcg agttcggacg ccagtccacg gtcggcgcga tggcgacgag
     3841 ccgcgacttc tcgaccagct cgaaccgggt cgggtcggtg gatctccgcc tgcgcctcgg
     3901 gggcaactgg gtgatcgatg cgcaggcgat gaccagccgc accacgcgcc tcgacggcga
     3961 gcggctggcg ggacccgcct acgtcgcgca ggtcggctac tacggccggt ccctgaactt
     4021 cgtcaccgag tacaccgatc ggagcccggg cttccgggcc gaccttggat tcatccagcg
     4081 ctcggacatc cgggagtggg agacggaggc cggctacaag tggttcccca agggcgggac
     4141 gctcctcagc tggggaccgg ggttcgaggc gactgtgaac tgggaccgca cgggccgcgt
     4201 ccaggactgg cgcgtgaacc ccgaggtcga gctcgagttc ccccgcaaca cgaacctgtt
     4261 cgccggggtg gactgggcct acgagcgctt cgcggggatc gggttcgaca agcgcgccgc
     4321 cttcgggttc ttcaacacgg agtggctgaa gtggctgtcg atctccctcg gcctgtcgcg
     4381 atcgcgcggg gtcaactact acccggcaca gggactgacg ccgttcctgg ccaatg
//
LOCUS       DAOPQX010000321         4433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21391_length_4433_cov_4.319324, whole genome shotgun sequence.
ACCESSION   DAOPQX010000321 DAOPQX010000000
VERSION     DAOPQX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21391_length_4433_cov_4.319324"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKK95_14190"
     CDS             <1..266
                     /locus_tag="PKK95_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19419.1"
                     /translation="RYVRLGRRAEAEELARRYDHMPYRRAIINAALGNRERMYHGLEQ
                     MAREETQRLVLLVRDPGFAPYRADDRYKNLLRRVHLEPGQQGG"
     gene            complement(263..3064)
                     /locus_tag="PKK95_14195"
     CDS             complement(263..3064)
                     /locus_tag="PKK95_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC19420.1"
                     /translation="MPVVPTSAQIVGIGYGALAGRTADPSGASLPGVTVTLSSDALMG
                     PRAAITGDDGTYLVAALPPGEYEVTFERAGFRPGSTRVRVTLGVTATVDAVLRVAGVN
                     ESVTVRPEAAVIDRHSSAVSETFTREQLAALPGSRSLFAVISFTPSLRVARFEAGVNT
                     GDAGGPFGSYGTRGSSRPMVEGIHVVQIFPLGFTIDYGAFEEVDVALAAQGPEWPVPG
                     VHLRFIARSGGNQYAGSVYGDVEHRAWQSHNIDLAQQWRGRDPQAGPLSRKANRLWQA
                     RDLNADLGGYLARDRAWWYGSFRDQEVQVRQVNFPVKPSETHLRSYTGKATVRAGAHR
                     FVGFLQAGRNHQPNRLDPFGPAGGRAINAVTAIHDSADATSALGVSGEISKVEWNAAF
                     GDRLFLEARVGRFAVRRSMTPNGDAPRFEDVDTLRVSGGGRQSERELGRNQFQASVSY
                     FRRGWIGSHHLKAGAELLDTLNEDRLNEAFPGGVLHVTRNGVPAEVYLFLAPSGSASG
                     LVAGSAYAGDSWRVADRLTLNLGLRFDRYRVFLPAQAPPPGAPPSAGYFAGDDDVVSW
                     NQLAPRLGVAFDASGDGRTILKGSAGRFWLAPGDLGVNPNATGWWRRHEWRDTNLSGS
                     WDDGEEGPLVDSRGGVALESVDPGLRAPWIDELTASLERALPGAIGVRTGVVWRRESR
                     QYARQNPSMPFSAFSEPVLIADPGPDGRAGTGDDGPGIPGRNLQIDGRPGPGASIVRN
                     VEGTASRHLTWEVTAHRRMRGRWSLSAGFAHTWTLEHANVYGGQQVRQNTLPLTPNDL
                     INTFAGGHHRLRTWTATASGTFRAPWRVDVTPLVRHQSGQPFGRTVSAVMNYGVIRVL
                     AEPVGTRRTDHVTLLDLRVERAVGRPGPRGVSVFLDVFNLLNSNAEAEVNWASGPAFL
                     QPLTVVPPRIARIGARARF"
     gene            3287..4216
                     /locus_tag="PKK95_14200"
     CDS             3287..4216
                     /locus_tag="PKK95_14200"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOC19421.1"
                     /translation="MRAKLAVCRGGYLFVVLACGVVSVGATSWDASSSQAAASDQARQ
                     GSAEPQIKAAHEQLLEAARKDDKATARRMMDDGLSWVHASGRIASKDELLGTTPAPPP
                     DVKVERVQLLGNAAIVTGSSRYEDGRQARFLQQWVNRDGQWKLLAHQGTPIGGMPAAG
                     QARATPSAEGTAGRATMKGSAPALASDEERAVWKADTQLRDAFLTGDVAAYEKLTSDD
                     FVRIGPGGERQDKAEFLQTVKRNAGQSAGRIETGEVSVGVSGDVARVVMTTWGAMPGG
                     DEFAPLRITKVLVKRNGQWQQAGVIFTPVTNQK"
BASE COUNT          692 a         1529 c         1597 g          615 t
ORIGIN      
        1 gcaggtacgt gaggctcggg cggcgcgccg aggcggagga gctcgcgcga cggtacgatc
       61 acatgcccta ccgccgggcg atcatcaacg cggcgcttgg caaccgcgag cggatgtacc
      121 acggcctcga gcagatggcc cgcgaggaaa cacagcgcct cgtgctcctg gtgcgggacc
      181 ctgggttcgc tccctaccgg gccgacgacc ggtacaagaa cctccttcgc cgcgtccacc
      241 tcgagccagg acagcagggg ggctagaagc gcgcgcgcgc gccgatgcgc gcgatccggg
      301 gcggcaccac cgtgagcggc tggaggaacg ccgggcccga tgcccagttc acctccgctt
      361 cggcattcga gttcagcagg ttgaagacgt ccaggaagac cgagaccccg cgcggtcccg
      421 ggcggccgac cgcgcgctcg acccgaaggt cgagcagcgt cacgtggtcc gtgcgccgcg
      481 tgccgacggg ctcggccagc accctgatga cgccgtagtt catcaccgcg gaaacggtac
      541 ggccgaaggg ctgtcccgac tggtggcgga cgagcggggt gacgtccacg cgccagggtg
      601 cgcgaaacgt ccccgacgcg gtcgctgtcc acgttcgcag gcggtggtgc ccgcctgcga
      661 acgtgttgat caggtcgttg ggcgtcagcg gaagcgtgtt ctggcggacc tgctggccgc
      721 cgtagacgtt ggcgtgctcc agcgtccagg tgtgagcaaa gccggccgac aacgaccagc
      781 gcccccgcat ccggcgatgg gccgtcacct cccacgtcag gtggcggctg gcggtccctt
      841 cgacgttgcg gacgatgctg gcgcccgggc caggccgccc gtcgatctgc agattgcggc
      901 ccgggattcc gggcccgtcg tcgcccgtcc cggcgcgtcc gtcggggccc gggtcggcga
      961 tcagcaccgg ctcgctgaag gccgagaagg gcatcgacgg gttctgtcgc gcgtactggc
     1021 gggactcgcg ccgccagacg actcccgtgc gaaccccaat cgcgcccggc agcgcgcgct
     1081 cgagcgacgc ggtcagctcg tcgatccagg gggcgcgcag gccgggatcg acggactcga
     1141 gggcgacgcc gccgcggctg tccacgagag ggccctcctc gccgtcgtcc cacgaaccgc
     1201 tgaggttggt gtcacgccac tcgtggcgcc gccaccatcc cgtggcattc gggttgacgc
     1261 cgaggtcgcc gggcgccagc cagaaccgtc cggcgctgcc cttgaggatc gtcctgccgt
     1321 cgccggaggc gtcgaaggca acgcccagcc tcggcgcaag ctggttccac gacacgacgt
     1381 catcatcgcc ggcgaagtac cccgccgatg gcggcgcgcc cggtggcggc gcctgcgccg
     1441 gcaggaagac gcggtagcga tcgaaccgga ggcccaggtt gagggtgagg cgatccgcca
     1501 cgcgccagga gtcgccggca tacgccgagc ctgccacgag gccgctggcg gatcccgagg
     1561 gggcgaggaa gaggtacacc tccgcgggca cgccgttgcg ggtcacgtgc agcacaccgc
     1621 cggggaaggc ttcgttcagc cgatcctcgt tgagggtgtc gaggagttct gctcccgcct
     1681 tgaggtggtg actgccgatc cacccccggc ggaagtagct gaccgacgcc tggaactggt
     1741 ttcgccccag ctcgcgctcg ctctgacgcc ctccgccgct gacgcgaagc gtgtcgacgt
     1801 cctcgaaccg gggcgcatcg ccgtttggcg tcatcgagcg gcgcaccgcg aaccggccaa
     1861 ccctcgcctc gaggaagagg cgatcgccga acgcggcgtt ccactcgacc ttcgagatct
     1921 cgccggacac gccgagagcg gaggtggcgt ccgcggagtc gtggatcgcg gtcaccgcgt
     1981 tgatggcccg tccgcctgcc gggccgaagg ggtcgaggcg attgggctga tggttgcggc
     2041 ctgcctggag aaatccgacg aacctgtgag cgccggcccg aaccgtggcc ttgcccgtgt
     2101 agctcctaag gtgggtttcc gacggcttga ccgggaagtt cacctggcgg acctgtacct
     2161 cctggtcgcg gaacgagccg taccaccacg cgcggtcgcg tgcgaggtag cccccgagat
     2221 ccgcgttcag atcgcgagcc tgccacaagc ggttggcctt gcgggacagc gggccggcct
     2281 gcgggtcccg cccgcgccac tgctgtgcga ggtcgatgtt gtgcgactgc caggctcgat
     2341 gctcgacatc gccatagacg ctgccggcgt attggttgcc gcccgaccgg gcgatgaacc
     2401 gaaggtgcac gccgggcacc ggccactcgg ggccctgcgc cgcgagggcg acgtccacct
     2461 cctcgaaggc gccgtagtcg atcgtgaacc cgagcgggaa gatctggacg acgtggatcc
     2521 cctcgaccat cggccggctc gacccgcgcg tgccgtacga gccgaagggc ccgccggcat
     2581 cccccgtgtt gacgcccgcc tcgaaccgcg cgacccggag gctcggcgtg aaggagatga
     2641 cggcgaagag gctgcgcgag ccgggaaggg ccgcgagctg ttcgcgggtg aaggtttcgc
     2701 tgacggcgct gctgtgccgg tcgatcaccg cggcctccgg ccgcaccgtc accgactcgt
     2761 tgacgccggc gactcgtagg accgcgtcca ccgtcgccgt cacgccaagg gttacgcgaa
     2821 cccgggtcga cccgggtcga aagcccgctc gttcgaaggt gacctcgtac tcgcctgggg
     2881 gcagcgctgc caccaggtag gtgccgtcat cgcccgtgat cgcagcccgg ggacccatca
     2941 aggcatcgct cgagagggtg acggtcacgc cgggtagaga ggcccctgac gggtcggccg
     3001 tccgccccgc gagcgcgccg tacccgatgc cgacgatttg agccgatgtg ggtacgactg
     3061 gcatggccac ggcaaacgcc gcggcgatca gccagcgtcc aatgctccag cttgtgacgc
     3121 gtgcccgcac cggcctctgc agcatgcaag tcgattgcct cgacccccgg accgacatcc
     3181 gccggacggt cgtcgacttg catgcccctg gccggcgagc ttcgggacgc acaggcctgc
     3241 cgcgctcgcc ggtcggtcac cccgtggaaa aacaggagac aagcgcatga gagccaagct
     3301 tgccgtttgt cgcggtgggt acctcttcgt cgtgctcgcc tgcggtgtcg tgtcggtcgg
     3361 cgctacatcg tgggatgctt cgtcatcaca agccgccgct tcggaccaag cccggcaggg
     3421 gagtgccgag ccgcaaatca aggcggccca cgagcagctg ctcgaagccg cgcgcaagga
     3481 cgacaaggct accgcccgcc ggatgatgga tgacggactg tcgtgggtcc acgcttcggg
     3541 gagaatcgcc agcaaggacg agcttctggg caccacgccg gctccgccgc ctgacgtgaa
     3601 ggtcgaacgg gttcagcttc ttggaaatgc agctatcgtg accggctcga gccggtacga
     3661 ggacggaagg caggcgcgct tcctgcagca gtgggtcaac cgcgacggac agtggaagct
     3721 cctcgctcac cagggcacgc ccattggggg gatgccagcg gcgggccagg cgcgcgcaac
     3781 gccatccgcc gagggcacgg cggggcgcgc cacgatgaag ggatccgcgc ccgcgctcgc
     3841 cagcgacgag gagcgggccg tgtggaaggc cgacacgcag ctgcgcgacg ccttcctcac
     3901 tggggacgtc gccgcctacg agaagctgac gtcggacgac ttcgtgcgca tcgggccggg
     3961 cggtgagcgt caggacaagg ccgagttcct gcagaccgtg aagcggaacg cgggacaatc
     4021 cgcaggccgg atcgagacgg gtgaggtcag cgtcggcgtc agcggggacg ttgcgcgggt
     4081 cgtgatgacg acctggggag cgatgcccgg cggcgacgag ttcgcacccc tacgcatcac
     4141 gaaggtgctc gtcaagcgga acgggcagtg gcagcaggcc ggcgtcatct tcacgccggt
     4201 gacaaatcag aagtagaaag acccctcctc acggcgggac gcctgtgcca ctgggccact
     4261 ggcaggcagg gacgccggcc ctacttggcc cgtagggacg acgcgccctg cttgctcggg
     4321 tcgccggtgg gcggcggggc ctggtcggtc gccgccggcg ggccgagcac cgcgggcgcc
     4381 ccgctggacg aggaccgccc cgccaagcgg atggtgttcg ccctcggcac cgc
//
LOCUS       DAOPQX010000322         4421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21472_length_4421_cov_3.987861, whole genome shotgun sequence.
ACCESSION   DAOPQX010000322 DAOPQX010000000
VERSION     DAOPQX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21472_length_4421_cov_3.987861"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PKK95_14205"
     CDS             complement(<1..634)
                     /locus_tag="PKK95_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC19422.1"
                     /translation="MSARILVIDDEPAIRDSLKMILEYEGYEFLGAPSGQDGIALVER
                     GSPDMVFLDVKMPGMDGIETLTRLRAIAEMLPVVMISGHATRETAFQAAKLGAFGFIE
                     KPLGREDVLLQVRNALEQGRLRAENRELRETAELKHEMIGNSPSLRVVTEAIRRAAPT
                     SATVLIQGESGVGKELVARAIHRNSLRKRERFVQVNCAAIPDELIESELFG"
     gene            complement(709..2190)
                     /locus_tag="PKK95_14210"
     CDS             complement(709..2190)
                     /locus_tag="PKK95_14210"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOC19423.1"
                     /translation="MNRRFALVTIALTAVIGFLTGLNVAGWLQGPERIQPVRPQAPVA
                     ARPTARLAAAGAVEFADIAERVNPAVVNIDATSRVQRARRGVLPPDLFGEPDRESPRD
                     SPETPRRGAGSGFIIDTAGYILTNHHVVEGAERITVKLADGRSVRARLVGADPDTDIA
                     LVKIDADRPLPVAALGDSSQLRVGDWVCAIGNPLGYEHTLTVGVVSYKGRKLFDPSLD
                     NYIQTDAAINFGNSGGPLLNARGEVVGINAAVSSRGSNIGFAVPINVAAANLEQLKRR
                     GRVVRGYIGVTLREVDADLRESLKLSSRDGALVEDVAALSPAERAGIRTYDMIVSIED
                     RPIGNNDDLIRSVSSFEPGSAVRLRLVRDGRPLDLAVKLAERPGRGASEDARRVSGEP
                     EDEARPAIGLDVRDLDRDIARRLGLRGDSRGALVVHVEPLSPAYDAEIRRGYVILEIN
                     RQLVASAADYRRAAGRARAGEVLTFLVHMTSGQRALRTVRVEP"
     gene            complement(2223..4232)
                     /gene="ligA"
                     /locus_tag="PKK95_14215"
     CDS             complement(2223..4232)
                     /gene="ligA"
                     /locus_tag="PKK95_14215"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOC19424.1"
                     /translation="MTPAERIAELRSQIRHHEERYYVLNDPEISDAEFDALLDELEAL
                     EREHPDLVTPDSPTQRVGGRPVEGFATVEHSVPMLSLDNAYSEDEVRAFDERARRGLG
                     AAGAALAYVTELKIDGLSISLVYEDGVLVRGVTRGDGQRGEDVTSNVRTVRAIPLHLR
                     EAVPGRIEVRGEVYLPRQAFERVNREREARDEPIFANPRNAAAGAIRNLDPRLVAKRG
                     LSSFVYQLLGGPGVVPDRHSATLDRLRALGLPVEPHWRLCRGVDEVLAYCAGWREERH
                     KLPFDTDGVVIKLDELALRRRLGATSKFPRWALAFKFPAEQATTRLHRIEVNVGRTGA
                     VTPYAVLEPVRVSGSTIGLATLHNEQEIARRDIREGDTVLVEKGGDVIPKIVKPILAM
                     RPPDSRPWRMPSACPECGSTLHKPEGEVVWRCVNSACPARLRRGLEHFASRRAMDIEG
                     LGEALIEQVIATGLVRDYADLYHLDVESLAALERMGKKSAQNLVGQIDRSRSNAPWRL
                     LYGLGIRHVGERAAQVLMAAFGSIEAVGRATVDELQAVDEIGPVVARSVREFFDEPRN
                     QELLRRLREAGVQTEAPRPAGGVDAPGRLTGKTFVLTGTLSSISRDEAAAAIEALGGK
                     VTGSVSKKTSWLVVGADAGSKLDKARALGVATLDEAAFLALVGRR"
BASE COUNT          569 a         1651 c         1561 g          640 t
ORIGIN      
        1 ggccgaacag ctccgactcg atcagctcgt cggggatggc ggcgcagttc acctgcacga
       61 agcgctcgcg cttgcgcaag ctgttccggt ggatcgcgcg cgcgaccagc tccttgccga
      121 cgccgctctc tccctggatg aggacggtcg cgctggtcgg cgcggcgcgc cggatcgcct
      181 cggtcaccac gcgcagcgac ggcgagttgc cgatcatctc gtgcttcagc tcggccgtct
      241 cgcgcagctc gcggttctcg gcgcgcaggc gtccctgctc gagggcgttc cgaacctgca
      301 gcagcacgtc ctcgcgcccg agcggcttct cgatgaagcc gaacgccccc agcttggcgg
      361 cctggaaggc ggtctcccgc gtggcgtgcc cggagatcat cacgaccggc agcatctcgg
      421 caatcgcgcg caggcgcgtc agcgtctcga tgccgtccat gccgggcatc ttcacgtcca
      481 ggaagaccat gtcgggcgac ccgcgctcca ccagggcgat gccgtcctgc ccggacggcg
      541 ccccgaggaa ctcgtacccc tcgtactcga ggatcatctt cagcgaatcg cggatggcgg
      601 gctcgtcgtc aatgaccagg atgcgcgcgc tcacgtcgct cctttgctgg actcacgtcg
      661 tcggtcgttc acgcgtcggt cacacacgcc gcacgcgggg gcctcgcgtc acggctccac
      721 cctcaccgtg cggagcgcgc gctggccgct cgtcatgtgc acgaggaagg tcagcacctc
      781 gcccgcccgg gcacggccgg cggcgcgccg gtagtcggcg gccgaggcca ccagctggcg
      841 gttgatctcc aggatcacgt agccgcggcg gatctccgcg tcgtaggccg ggctcagcgg
      901 ctcgacgtgc accaccagcg ccccccggct gtcgcccctc agccccagcc gccgcgcgat
      961 gtcgcggtcg aggtcgcgca cgtcgaggcc gatggccgga cgggcttcgt cctccggctc
     1021 gcccgacacg cgccgggcgt cctcggaggc tccgcggccc ggccgttcgg ccagcttcac
     1081 ggccaggtcg agcgggcggc cgtcgcgcac gaggcggagg cggacggccg agcccggttc
     1141 gaaggaggac accgaccgga tgaggtcgtc gttgttcccg atgggccggt cctcgatcga
     1201 gacgatcatg tcgtaggtcc ggatgccggc gcgctcggcg ggcgacaggg cggcgacgtc
     1261 ctcgacgagc gccccgtcgc gggaggagag cttgagcgac tcccggaggt cggcgtcgac
     1321 ctcgcgcagc gtgacgccga tgtagccgcg caccacccgc ccgcggcgct tgagctgctc
     1381 gaggttggcg gcggccacgt tgatcggcac cgcgaacccg atgttgctgc cgcgcgagct
     1441 gaccgcggcg ttgatcccga ccacctcgcc gcgcgcgttg agcagcggcc ccccgctgtt
     1501 cccgaagttg atggcggcgt cggtctggat gtagttgtcg aggctcgggt cgaacagctt
     1561 ccgccccttg tagctcacca cccccacggt gagcgtgtgc tcgtagccga gggggttgcc
     1621 gatcgcgcag acccagtcgc ccacccgcag ctgcgacgag tcgccgagcg ccgccaccgg
     1681 cagcgggcgg tcggcgtcga tcttcaccag ggcgatgtcg gtgtcggggt cggccccgac
     1741 cagccgcgcg cgcacgctgc ggccgtcggc cagcttcacg gtgatccgct cggccccctc
     1801 gacgacgtgg tggttggtga ggatgtagcc ggccgtgtcg atgatgaagc cgctgcccgc
     1861 cccccgccgc ggcgtctcgg ggctgtcgcg cggcgattct cgatcgggct ccccgaagag
     1921 gtcgggcggc agcacccccc ggcgcgcccg ctgcacccgc gaggtggcgt cgatgttcac
     1981 cacggcgggg ttgacccgct cggcgatgtc ggcgaactcc accgcgccgg cggccgccag
     2041 ccgcgcggtt gggcgcgcgg ccaccggcgc ctgcggccgg accggctgga tccgctcggg
     2101 cccctgcagc cagcccgcga cgttcaggcc cgtgaggaac ccgatcaccg cggtcagcgc
     2161 gatggtgacg agggcaaagc gccggttcat gtgtcgatta taagcacggg gcgcgccgag
     2221 gctcagcgcc ggcccaccag cgcgagaaac gccgcctcgt ccagcgtggc cacgcccagg
     2281 gcccgggcct tgtcgagctt gctgccggcg tcggcgccca ccaccagcca gctggtcttc
     2341 ttgctgaccg acccggtgac cttgcccccg agcgcctcga tggccgccgc cgcctcgtcg
     2401 cgcgatatcg acgacagcgt cccggtgagc acgaacgtct tccccgtcag ccgcccgggc
     2461 gcgtcgacac cgcccgcggg ccggggcgcc tcggtttgga cgcccgcctc gcgcagccgc
     2521 cgcagcagtt cctggttgcg gggctcgtcg aagaactcgc gcaccgaccg cgccacgacg
     2581 gggccgatct cgtcgaccgc ctgcagttcg tccacggtgg cccgccccac cgcctcgatc
     2641 gacccgaatg cggccatcag cacctgtgcc gcgcgctcgc cgacgtggcg gatccccagc
     2701 ccgtacagca ggcgccacgg cgcgttcgac cggctgcggt cgatctggcc gaccaggttc
     2761 tgcgccgact tcttccccat ccgctcgagg gcggccagcg actccacgtc gagatggtag
     2821 aggtctgcgt agtcgcgcac gaggccggtg gcgatcacct gctcgatcag cgcctcgccg
     2881 agcccctcga tgtccatcgc ccggcgcgag gcgaagtgct cgaggccgcg gcgcagccgc
     2941 gccgggcagg cgctgttgac gcagcgccac accacttcgc cctcgggctt gtgcagcgtg
     3001 ctgccgcatt ccgggcaggc agaaggcatg cgccacgggc ggctgtcggg cggccgcatc
     3061 gccaggatcg gcttgacgat cttcgggatg acgtcgcccc ccttttcgac gagcacggtg
     3121 tcgccctcgc ggatgtcacg ccgggcgatc tcctgctcgt tgtggagggt ggcgaggccg
     3181 atcgtcgagc ccgacacgcg gaccggctcg aggaccgcgt agggggtcac ggccccggtc
     3241 cggcccacgt tgacctcgat tcggtgcagc cgcgtggtgg cctgttcggc cgggaacttg
     3301 aacgcgagcg cccagcgcgg gaacttcgag gtcgccccca gccgccggcg cagcgccagc
     3361 tcgtcgagct tgatgaccac cccatcggtg tcgaacggca gcttgtggcg ttcctcacgc
     3421 cacccggcgc agtaggccag cacctcgtcc accccgcggc agagcctcca gtggggctcg
     3481 acgggaaggc cgagcgcgcg gagccggtcg agcgtggccg agtggcggtc gggcaccacg
     3541 cccggccccc ccagcagctg gtagacgaac gacgagaggc cgcgcttggc caccaggcgc
     3601 gggtcgaggt tgcggatggc gccggcggcc gcgttgcgcg ggttcgcgaa gatcggctcg
     3661 tcgcgcgcct cccgctcgcg gttcacccgc tcgaaagcct gccggggcag gtagacctcg
     3721 ccgcgcactt cgatccggcc cggcaccgcc tcgcgcagat gcagcgggat ggcgcgcacc
     3781 gtcctcacgt tcgacgtgac gtcctcgccg cgctgcccgt cgccgcgggt caccccgcgc
     3841 accaggacgc cgtcctcgta gacgagcgag atgctcaggc cgtcgatctt cagctcggtc
     3901 acgtaggcga gcgccgcgcc ggccgccccg agcccccggc gcgcccgctc gtcgaaggcg
     3961 cgcacctcgt cctcgctgta cgcgttgtcg agcgagagca tcggcaccga gtgctcgacg
     4021 gtcgcaaacc cctcgacggg cctgcccccg acccgctgcg tgggcgaatc gggggtcacc
     4081 aggtcggggt gctcgcgctc gagcgcttcg agctcgtcga gcagggcgtc gaactcggcg
     4141 tcggagatct ccggatcgtt gaggacgtag taccgctcct cgtgatgccg gatttgggat
     4201 cgcagctccg cgatgcgttc ggcaggagtc atggcaggtc ggtggaccgg agttcgccgc
     4261 gccgccggcc cgctagcggt cgcgcgcgag cacgaagtcg ggcagggcca tcagcgcgtc
     4321 ccgatcgggt ccgggcggga acacctcgag gctgcgccgc gcggcggtcg cgtactcggc
     4381 ggcccgtgcc atcgcgtact ccacggcccc ggtctcggcc a
//
LOCUS       DAOPQX010000323         4420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21482_length_4420_cov_3.333562, whole genome shotgun sequence.
ACCESSION   DAOPQX010000323 DAOPQX010000000
VERSION     DAOPQX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21482_length_4420_cov_3.333562"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1332)
                     /locus_tag="PKK95_14220"
     CDS             complement(91..1332)
                     /locus_tag="PKK95_14220"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOC19425.1"
                     /translation="MPVHIVGGGAAGLATAIFLARRRPALRIAVLDGARRLGAKILVS
                     GGGRCNLTNRVVTASDYWGGDRRIVASVLAAFPVEATIAFFREIGVSVHEEESGKLFP
                     DTNRARTVLDALLDEAGRLGVTIVTGERVTRLEPLLAAGPVVLATGGRSLPKSGSDGE
                     GLRMAASLGHTIVETTPALAPLVVGCGLSRAVSSLSGKEGTAAFHQQLSGVSHDAALT
                     VEHGGRQLARLRGPLLWTHFGVSGPVALDASRHWHRAALGQEAVRVRVSFLPALDARG
                     AEQALLEAAGRRPAASLRSTLASLVPSAVADAVLAHLSIEPAVQMAQLPRATRAVLAK
                     ALVGWDLAVTGSRGYNFAEATAGGVALDEVNRATLESRVRPGLYLVGEMLDVDGRLGG
                     FNFQWAWSSAWVAARGIATGR"
     gene            complement(1644..1964)
                     /locus_tag="PKK95_14225"
     CDS             complement(1644..1964)
                     /locus_tag="PKK95_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19426.1"
                     /translation="MDKRPKATRGKGVEIVTAARDEAHEGSDVSTMVEGILDVLERHE
                     ADANQGVLSLLTAFMQAADRLLDVSTPEEMAHNREALLGMMDHAKKFVESWPHQTPAH
                     WRVH"
     gene            complement(2129..2779)
                     /locus_tag="PKK95_14230"
     CDS             complement(2129..2779)
                     /locus_tag="PKK95_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOC19427.1"
                     /translation="MSSQLLEQITAAVVEGDPDATVAGTREALAAGVEPLRIINEGLV
                     PGMRTVGDKYSSGEYFLPNLIVSAAGMKQAMALLEPELKARRETVQSAGTVVIGTVRG
                     DIHEIGKSLVATMLSANGFEVHDLGVDVSAERFVDTVRETGAGLVGLSALLTTTMMGQ
                     KKVIEELERAGLRGGVKVMVGGAPVSQAFADTIGADGYAQDAVAAVELATRLASRR"
     gene            complement(2839..4308)
                     /locus_tag="PKK95_14235"
     CDS             complement(2839..4308)
                     /locus_tag="PKK95_14235"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOC19428.1"
                     /translation="MRPTVRVLSEELVERIVGEALALLREPGVRVHNEEALRLLAEAG
                     AEVDRGALVARIPEKIVRDALGSVPKAFDLYTLDGRPAVHYGGDHVHFDPGSAALAIL
                     DPATGQERVPVTADFVRLVKLVERLPQLDAQSTSMICGDVAAEIGDLYRLYLALNFIR
                     KPIITGAFRTDTWWVMKEMLALVAGGEEALAARPIAVFDVCPSPPLLWSDLTCQNLID
                     CARAGIPAELVSMPLAGATAPVTLAAAVVQHAAENLSGITIGQLARRGAPIVWGGSPA
                     AFDMREGTTPMGDVGTWMIDCAYTQVGKHLGLPTHAYLGMSDAKVLDAQCGMESMGGT
                     VMAALAGVNMVSGAGMLDFESCQSLEKLVLDAEMIGMAKRLIRGIEPREEPIALDLFK
                     RHGHQADYLADPHTLRWFAKELYLPSAVIDRSTYDGWKRKGAKTTAERAAERVDQLVA
                     SYEQPSMSSEVRTELRSIATRAAQKFGMKELPELAELPE"
BASE COUNT          659 a         1613 c         1487 g          661 t
ORIGIN      
        1 cgctggggtg gcgttcggct tcgcgcgaaa agcacttgtc acgcgtgtcg cgctctgaca
       61 acgaaccctc tgcgcctgcg ttgaccctcc tcatcgcccg gtcgcgattc cgcgcgccgc
      121 gacccaggcg ctcgaccagg cccactggaa gttgaagccg ccgaggcggc catccacgtc
      181 gagcatctcg cccaccaggt acagccccgg gcgcacccgc gattcgagcg tcgcccggtt
      241 cacctcgtcg agcgcgaccc cgccggcggt ggcctcggcg aagttgtagc cccgcgaccc
      301 ggtgaccgcc aggtcccacc cgacgagggc cttggcgagc accgcgcgcg tggctcgcgg
      361 aagctgcgcc atctgcactg ccggttcgat ggacaagtgg gcgagcacgg cgtcggccac
      421 ggcggagggg accagagagg ccagcgtcga gcggaggctt gccgcaggcc gcctcccggc
      481 agcctcgagc aacgcctgct cggccccacg ggcatcgaga gcgggaagga acgacacccg
      541 cacgcgcacg gcttcctggc ccagcgccgc gcggtgccag tgccgcgacg cgtcgagcgc
      601 cacgggcccg ctgacgccga agtgcgtcca cagcagcggc ccccgcaggc gggcgagctg
      661 ccggccgccg tgctccaccg tcagcgccgc gtcgtgcgac acgcccgaca actgctggtg
      721 gaacgcggcc gtgccctcct tgccggaaag gctcgacacc gcgcgcgaca ggccgcagcc
      781 gaccacgagc ggcgcaagcg ccggcgtcgt ctcgacgatc gtgtggccga gcgaggccgc
      841 catccggagg ccctcgccgt cgctgcccga cttcggcagc gagcggccgc ccgtcgccag
      901 cacgacgggg ccggcggcaa gcagcggctc caggcgggtc acccgctcgc cggtgacaat
      961 cgtcacgccc agccggcccg cctcgtcgag cagggcgtcg agcacggtgc gggccctgtt
     1021 ggtgtcggga aagagcttgc ccgactcctc ctcgtggaca ctcacaccga tctcgcggaa
     1081 gaaggcgata gtcgcctcca ccgggaaggc cgcgagcacc gacgcgacaa tgcgccggtc
     1141 gccgccccag tagtcggacg cggtgacgac acggttggtg aggttgcagc ggccgccgcc
     1201 gctcacgagg atcttggcgc cgaggcgcct ggccccgtcg aggacggcaa tgcggagagc
     1261 cgggcggcgc cgcgcgagga agatcgcagt ggccagcccc gccgcgccgc cgccaacgat
     1321 gtgcaccggc atcgcgggtc agactatcac gccgcatctg tcaagggtgt cacactgcat
     1381 tcccgatcac gttcaacgca ggggggcaga gggccctcaa cccagcccct acccccggcg
     1441 ctctgctgcg ccgggcagcg cgtgagcggg tgacgcgggg cgcgtcctcg atacgtgtga
     1501 gtagcgaggt tggctcgagg agggattcag caacagcgcg ggggcgcaag tgacgacggt
     1561 ctgtcgggga gagttgtccg aggtgccgga acgggaaggg ttttctctgt gtctctgcgt
     1621 ctctgtgttg aacgtgacgt gcgtcagtgc actcgccagt gcgccggcgt ctgatggggc
     1681 cagctttcga cgaacttctt cgcgtggtcc atcatcccga gcagcgcctc gcggttgtgc
     1741 gccatctcct cgggggtgct gacgtcgagc agccggtcgg ccgcctgcat gaaggccgtc
     1801 agcagcgaca gcactccctg gttggcgtcc gcctcgtggc gctcgagcac gtcgaggatt
     1861 ccctccacca tcgtcgaaac gtcgctgccc tcgtgggcct cgtcccgggc cgcggtcacg
     1921 atctcaacgc ccttgcccct ggtcgccttc ggccgcttgt ccatgcgcct tccttgttcg
     1981 ccaacccacg acgccggttc accccgggag gctgcaagac cgccgccaag cagccttcac
     2041 gagaaacccg tgcgttccgc tgtccgcggc cggcccgact ccgatttcgt ggaccaaaag
     2101 tcgcggattc ccgcactacg ctgccgggct agcggcggct cgccagccgc gtggccagtt
     2161 ccaccgccgc caccgcgtcc tgggcgtagc catcggcgcc gatcgtgtcg gcgaaggcct
     2221 ggctgacggg agcgccgccg accatcacct tcacgccgcc ccgaagcccc gcgcgctcca
     2281 gctcctctat gaccttcttc tgccccatca tcgtcgtcgt cagcagcgcc gagaggccga
     2341 ccagccccgc gccggtctcc ctcaccgtgt cgacgaaccg ctcggccgag acgtccacgc
     2401 cgaggtcgtg cacctcgaaa ccgttggccg agagcatcgt cgccaccagc gacttgccga
     2461 tctcgtggat gtcgccgcgc accgtcccga tgacaaccgt gccagcagac tgcacggttt
     2521 cgcgccgggc cttcagctcg ggctcgagca gcgccatcgc ctgcttcatg ccggccgccg
     2581 acacgatcag gttcgggagg aagtactcgc ccgacgagta cttgtcgccc acggtgcgca
     2641 tgccggggac cagcccctcg ttgatgatgc gcagcggctc gacgcccgcg gccagcgcct
     2701 cgcgggtgcc ggccaccgtg gcatccgggt ccccttccac gacggcggca gtgatctgtt
     2761 cgaggagttg agaggacatg ttcagttgtc agttttcagt tgtcagtgtt cgtcttccgc
     2821 aggctcggca ggcggcgcct actccggcag ctccgccaac tccggcagct ccttcatgcc
     2881 gaacttctgc gccgcgcgcg tggcgatcga ccgcagctcg gtgcgaacct ccgatgacat
     2941 ggacggctgc tcgtacgacg ccaccagctg atcgacgcgc tcggcggcgc gctcggccgt
     3001 tgtcttcgct cccttgcgct tccagccgtc gtaggtcgag cggtcgatga cggcggacgg
     3061 gaggtacagc tccttcgcga accagcgcag cgtgtgcggg tcggcaaggt agtcggcctg
     3121 gtgcccgtgc ctcttgaaca ggtcgagggc gatcggctcc tcgcgcggct cgatgccgcg
     3181 aatcagccgc ttggccatcc cgatcatctc ggcgtcgagc acgagcttct cgaggctctg
     3241 gcagctctcg aagtcgagca tgccggcgcc cgacaccatg ttcacgccgg cgagggcggc
     3301 catcaccgtc ccccccatcg actccatgcc gcactgggca tcgagcacct tggcgtcgct
     3361 catgccgaga tacgcgtgcg tcggcaggcc gaggtgcttg cccacctgcg tgtacgcgca
     3421 gtcgatcatc caggtcccga cgtcgcccat gggggtcgtg ccctcccgca tgtcgaaggc
     3481 ggctggcgaa ccgccccaga caatcggggc gccgcgccgc gcgagctggc cgatggtgat
     3541 gccactcagg ttctctgcgg cgtgctgcac gaccgcggcg gccagggtga ccggcgcggt
     3601 cgcgccggcc agcggcatcg agaccagctc ggccgggatc cccgcccgcg cgcagtcgat
     3661 gaggttctgg caggtgaggt cggaccacag cagcggcggc gacgggcaga cgtcgaacac
     3721 ggcaatcggg cgcgcggcga gtgcctcttc cccgcccgcg accagcgcga gcatctcctt
     3781 catcacccac caggtgtcgg tcctgaacgc gccggtgata atcggtttcc tgatgaagtt
     3841 cagcgcaagg tagaggcggt agaggtcgcc gatctcggca gccacgtcgc cgcagatcat
     3901 cgacgtgctc tgcgcgtcga gctgcgggag acgctccacc agcttcacga ggcggacgaa
     3961 gtcggccgtc acgggcaccc gctcctggcc tgtcgcggga tcgaggatgg cgagcgccgc
     4021 cgacccgggg tcgaagtgca cgtggtcgcc gccgtagtgc accgccgggc ggccgtcgag
     4081 cgtatagagg tcgaacgcct tcggcaccga gccgagcgcg tcacggacga tcttctcggg
     4141 gatccgggcg accagcgccc cccgatccac ctcggccccc gcctcggcca gcaggcgcag
     4201 ggcctcctcg ttgtgcacgc ggacgccggg ctctcgcagc agcgccaagg cctcgccgac
     4261 gatccgctcg accagctcct cgctcagcac ccgcaccgta ggacgcatat cggcgaatcg
     4321 tagtgcgcaa ggccacttgc gcgcaagcgc gcagatcggg gcgggtgaaa agtactcggc
     4381 tgtcgcgaaa ggctacgcca gacggctcgg agaagccgtt
//
LOCUS       DAOPQX010000324         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21588_length_4404_cov_4.544033, whole genome shotgun sequence.
ACCESSION   DAOPQX010000324 DAOPQX010000000
VERSION     DAOPQX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21588_length_4404_cov_4.544033"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..653)
                     /gene="hpt"
                     /locus_tag="PKK95_14240"
     CDS             complement(114..653)
                     /gene="hpt"
                     /locus_tag="PKK95_14240"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOC19429.1"
                     /translation="MPDRTWDRGLSVLLTADQISARVTEMAREIAGLYEPDEDIHLVA
                     ILKGAFLFLGDLVRAMDRHVTLDFMAVSSYGKGTRSSGEVRMVKDLDAGLEGRNVIIV
                     EDIVDTGLTLAYLQEILRARSPKSLRTACLLSKPSQRKVDVKVEHVGFTIEDRFVVGY
                     GLDYGERYRNLPYIGVISG"
     gene            772..1476
                     /gene="deoC"
                     /locus_tag="PKK95_14245"
     CDS             772..1476
                     /gene="deoC"
                     /locus_tag="PKK95_14245"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOC19430.1"
                     /translation="MSSHSSTTPEQVARLIDHTLLKAEATPADIDRLCDEASVAGFYA
                     VCVNPVFVARAARRLRQAPVVICSVAGFPLGAVRTEVKALEARRAIEDGAGEVDMVIH
                     VGALKAGETAAVRDDIAAVVDACRRGAARCKVILETALLDEPLILAGCRAAVEAGADF
                     VKTSTGFGPSGAKAEDVALMARTVREAGLGVKAAGGIRSYAQFREMVAAGATRVGTSA
                     GLRILEEARGGIVPPR"
     gene            1508..1777
                     /locus_tag="PKK95_14250"
     CDS             1508..1777
                     /locus_tag="PKK95_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19431.1"
                     /translation="MAKELSKDVLNRLARLGAQARLDELEAERRAILKAFPGLGGGRK
                     PAAVETPAAKPPKKARKKRNVSAAARKAASERMKQYWAAKKQAQP"
     gene            1805..3145
                     /locus_tag="PKK95_14255"
     CDS             1805..3145
                     /locus_tag="PKK95_14255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HOC19432.1"
                     /translation="MARAITWLSDGVWGPWNTAIGPVPGPLVLLLLGVGLFLTLRYRF
                     VQVRRFPAALRAIVGQQSGTGGVLTPFQAFMTALAASIGTGNIAGVATAVVAGGPGAV
                     FWIWCYGFFATAIKFTEAVLGTTFRLSRAGGVASGPMYYLRDGLGRPRLAAAYALVAG
                     IAALTTTPFTQPNSIAVAFESEFALSPLASGVLIAVLTWLVIIGGVKSIGRAVEKLSP
                     LKVFLYLAGGLFVILVNADRLPEVFEAIVREAFSLRAAAGSATGMGVMVAMRYGLARG
                     VYANEAGYGTAAVAYGTARSSEPLQQGLNAIIEVVVVSFVTSSISALSILVTGAWQSG
                     LNSTAAVAYAFDSAIPVAGGPIVAVCAFLFGYTTLIGWAYYGEQFFEYILGLRVTVPY
                     RWIYCGLIVFGATARVETVWAWGDLMNGLQVFPNMVGVLGLSGLVAARLAKRKT"
     gene            complement(3170..3243)
                     /locus_tag="PKK95_14260"
     tRNA            complement(3170..3243)
                     /locus_tag="PKK95_14260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3209..3211),aa:Gly,seq:ccc)
     gene            complement(3319..>4404)
                     /locus_tag="PKK95_14265"
     CDS             complement(3319..>4404)
                     /locus_tag="PKK95_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOC19433.1"
                     /translation="DGGAQITASHNSKQYNGMKMVGRGAFPLSGDAGISDIRDMLVNG
                     TLPMPAPRPGAVETRDVLDDYVRHVLGFIDPAVIKPFDVVLDAGCGIAGLVAPKLFAG
                     LPCRTTELCFTIDGTFPTHEANPLIEENRRDIVEQVVRQGADIGIAWDGDADRCFFID
                     GKGGFVPGDFITALLAEAFLIRHPGATIIYDLRASYAVKDTVARYGGRALMNRVGHAF
                     IKRRMREENAIFGGEVTGHYYFRDNFYADNGFIPAMLILELMSRKGQTLEALLAPLHE
                     QYFISGEINTKLASMEAAAEKLAGLAARYADATVYEMDGVSVEYPDWHFNVRPSNTEP
                     LLRLNLEAKTPELMAEKRDEVLAYVRS"
BASE COUNT          629 a         1472 c         1600 g          703 t
ORIGIN      
        1 gaccatcgtg cgggggaagg tgctgatgaa ggatcggcgc gtgctcacgc tcgacgagaa
       61 ccgcgtcctc gccgacgcgc gccggctggc cgccgaggtg aaggcggcgg tcgtcagccg
      121 ctaatcacgc cgatgtaggg gaggttgcgg taccgctcgc cgtagtcgag gccgtacccc
      181 accacgaagc gatcctcgat cgtgaagccg acgtgctcga ccttcacgtc caccttccgc
      241 tgcgagggct tgctcagcag gcacgccgtc cgcagcgact ttggcgaccg cgcccgcagg
      301 atctcctgga ggtaggcgag cgtcaggccg gtgtcgacga tgtcttcgac gatgatgacg
      361 ttgcggccct cgaggccggc gtcgaggtcc ttgaccatcc gaacctcgcc agaggaccgc
      421 gtcccctttc catagctgga gaccgccatg aagtcgaggg tgacgtgccg atccatcgct
      481 cgaaccaggt cgccgaggaa gaggaacgcc cccttgagga tcgcgacgag gtggatgtct
      541 tcatcgggct cgtagaggcc ggcgatttcg cgggccatct cggtgacgcg ggcgctgatc
      601 tggtcggcgg tcagcaggac ggagaggcca cggtcccacg ttcgatcggg catgacgctg
      661 gagcctagca cacttcccga ttgaagtggc tacagcgccc gctatatgct gcggtgctgg
      721 cgcggggcct acccggtccc ggcgccgggg gattcccggg tgacgatcgc catgtcctcc
      781 cactcgtcga cgacgccgga acaggtcgcc cggctgatcg accacacgct gctgaaggcc
      841 gaggcgacgc cggccgacat cgaccgcctc tgcgacgagg cgtcggtcgc tgggttctac
      901 gccgtctgcg tcaacccggt cttcgtcgcg cgggccgcac gccgcctcag gcaggcgccg
      961 gtcgtgatct gcagcgtcgc cggcttcccc ctcggggccg tgcgcaccga ggtgaaggcg
     1021 ctcgaggcgc ggcgggccat cgaggacggc gccggcgagg tggacatggt gatccacgtg
     1081 ggcgccctga aggcggggga gacggcggca gtgcgcgacg acatcgccgc ggtggtggac
     1141 gcgtgccgtc gcggagccgc ccgctgcaag gtcatcctcg agacggcgct gctcgacgag
     1201 ccgctgatcc tcgcgggctg ccgcgccgcc gtcgaggcgg gggccgattt cgtgaagacc
     1261 tcgacgggct tcggtccctc gggggcgaag gccgaagacg tggcgctcat ggcgcggacg
     1321 gttcgcgaag ccgggctcgg ggtgaaggcc gccgggggca tccgcagcta cgcgcagttc
     1381 cgcgagatgg tggcggcagg agcgacgcgg gtggggacca gtgccgggct tcggatcctc
     1441 gaggaggctc ggggcgggat cgttccgccc cggtgacgcg gcgccctgcc gcggaaaagg
     1501 agaacgtgtg gcgaaggaac tgagcaagga cgtgttgaac cgcctggccc ggctgggggc
     1561 ccaggcgagg ctggacgagc tcgaggcgga gcgtcgggcg attctcaagg cattcccggg
     1621 tcttggcggc gggcgcaagc cagcggcggt ggagacgccg gcggccaagc cgcccaagaa
     1681 ggcgcgcaag aagcggaacg tttcggccgc cgcgcgcaag gcggcgtccg aacggatgaa
     1741 gcagtactgg gccgcgaaga agcaggcgca gccctagcgc cgcccgcaaa ggtcgatgcc
     1801 tgacttggcg cgggcgatta cctggctgag cgacggcgtc tgggggccct ggaacaccgc
     1861 catcggcccg gtgcccggcc cgctcgtcct gctgctgctg ggcgtcggcc tgttcctgac
     1921 actccgttac cgcttcgtcc aggtccggcg cttccccgcg gccctccgcg ccattgtcgg
     1981 gcagcagtcg gggaccggcg gcgtcctgac gccgttccag gcgttcatga cggcgctcgc
     2041 cgcctcgatc gggaccggca acatcgccgg cgtggcgacg gcggtggtcg cgggggggcc
     2101 cggcgcggtc ttctggatct ggtgctacgg cttcttcgcc accgccatca agttcaccga
     2161 ggccgttctc ggcaccacct tccgcctctc gcgggccggc ggcgtcgcgt ccggcccgat
     2221 gtactacttg cgagacggcc tcgggaggcc gcgcctggcg gcggcgtacg ccctggtcgc
     2281 cggcattgcg gcgctcacca cgaccccctt cacgcagccc aactcgatcg cggtggcgtt
     2341 cgagtcggag ttcgcgctct cgccgctcgc gtcgggcgtc ctcatcgcgg tcctcacctg
     2401 gctggtgatc atcggcggcg tgaagtcgat cggccgggcg gtggagaagc tctcgccgct
     2461 gaaggtgttc ctctacctgg cggggggcct cttcgtgatc ctcgtgaacg cggaccgcct
     2521 gcccgaggtc ttcgaagcga tcgtccgaga ggcgttctcg ctgcgcgccg ccgcgggcag
     2581 cgcgaccggc atgggggtga tggtggcgat gcgctacggg ctggcgcgcg gcgtctacgc
     2641 gaacgaggcc ggctacggca cggccgccgt ggcgtacggc accgcgcgca gcagcgagcc
     2701 gctgcagcag ggactgaacg cgatcatcga ggtggtcgtc gtgtcgttcg tcacctcctc
     2761 gatcagcgcc ctgtcgatcc tcgtcaccgg cgcctggcag tcgggcctca acagcacggc
     2821 tgccgtcgcc tacgccttcg actcggccat cccggtggcg ggcggcccga tcgtcgccgt
     2881 ctgtgccttc ctgttcgggt acaccaccct catcggctgg gcgtattacg gcgagcagtt
     2941 cttcgagtac atcctgggcc tgcgcgtcac ggttccctac cgctggatct actgcggcct
     3001 gatcgtgttc ggcgccacgg cgcgcgtgga gacggtgtgg gcctggggag acctgatgaa
     3061 cgggctgcag gtgttcccga acatggtcgg ggtcctgggc ctcagtgggc tcgtggccgc
     3121 gcgcctggcg aagaggaaga cgtaggaggg agaacgcgag cgcggaaggt ggagcgggct
     3181 acggggatcg aacccgtcta actggcttgg gaagccaggg cattaccact atgctaagcc
     3241 cgcccgaacc ctgacattgt aagggcaggg cggcggcgct gcaagcccgc cgccggcctg
     3301 cccctcgccc gtccgctctc aggaacggac gtaggccaac acctcgtccc gtttctccgc
     3361 catcagttcc ggcgtcttcg cctcgaggtt cagccgcagc agcggctcgg tgttcgacgg
     3421 ccggacgttg aagtgccagt cgggatattc gaccgacacc ccgtccatct cgtagaccgt
     3481 cgcgtccgcg tagcgggcgg cgaggccggc cagcttttcg gcagccgcct ccatcgaggc
     3541 gagcttggtg ttgatctcgc ccgagatgaa gtactgctcg tgcagcggcg ccagcagcgc
     3601 ctccagcgtc tgccccttcc gcgacatcag ctcgaggatc agcatcgcgg gaatgaagcc
     3661 gttgtcggcg tagaagttgt cgcggaagta gtagtgtccc gtgacctcgc cgccgaagat
     3721 ggcgttctcc tcgcgcatcc gccgcttgat gaacgcgtga ccgacgcggt tcatgagcgc
     3781 gcggcccccg tagcgcgcca cggtgtcctt caccgcgtag ctggccctga ggtcgtagat
     3841 gatcgtcgcg ccggggtggc ggatgaggaa ggcctcggcc agcagcgccg tgatgaagtc
     3901 gccggggacg aagccgccct tgccgtcgat gaagaagcag cggtccgcgt cgccgtccca
     3961 cgcgatcccg atgtcggccc cctggcgcac gacctgctcg acgatgtcgc ggcggttctc
     4021 ctcgatgagc gggttggcct cgtgggtggg gaaggtcccg tcgatcgtga agcacagctc
     4081 ggtcgtccgg caggggaggc cggcgaagag cttcggcgcg acgagcccgg cgatgccgca
     4141 ccccgcgtcg agcacgacgt cgaacggctt gatcacggcc gggtcgatga agccgagaac
     4201 gtgccggacg tagtcgtcga gcacgtcgcg cgtctcgacg gcgcccggcc gcggggcggg
     4261 catcggcagc gtgccgttca ccagcatgtc gcggatgtcg gagatccccg cgtcgccgga
     4321 cagcggaaag gccccgcgcc cgaccatctt catgccgttg tactgcttgg agttgtgcga
     4381 ggcggtgatc tgcgcgccgc cgtc
//
LOCUS       DAOPQX010000325         4383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21721_length_4383_cov_4.383549, whole genome shotgun sequence.
ACCESSION   DAOPQX010000325 DAOPQX010000000
VERSION     DAOPQX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21721_length_4383_cov_4.383549"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="PKK95_14270"
     CDS             complement(<1..2351)
                     /locus_tag="PKK95_14270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC19434.1"
                     /translation="MPLEPVFTPRGQLLASGSDLEPAALAEIRDPTSQVAPRLAAAFE
                     RGHGEGLLQLGAGEVTTPLPPAWDYWRDFAARFVSALCAQPESPHVAPPPDEVLETTA
                     AAVPPMAGAEYVTAASLRALWTDLDAAFATRLAESGAAVQDVLRTFNGAWNTVGRVHF
                     HLAENRRDPDAPFAFLATYTQGLSSQGKPRHVPLGRALEEYGGAANRGALLSLLVPVQ
                     RAAERCAWLKAMVESGEVFHPLRWTPAEALNFLRDVHVLEDAGVIVRVPAHWKAGRPP
                     RPTVVARVGAKPAAGGLGLESLLDFHVEVTLDGERLTAEEIRSLLRATEGLALLRGRW
                     VEIDRERLGGMIDRFSRIEQAAAREGLSFVEAMRLVAGADPRLQEAADEEDRAWAGVE
                     AGPWLAGVLQRLRSPDALGGVDPGEAFQGSLRPYQQVGVRWLHFLSSLGLGACLADDM
                     GLGKTVQVLALLAAIERRKTGCGGTLLVAPASVLPNWAAEIARFTPTLSTFVAHPSAV
                     PAAELKSPDPERLARADIVLTTYGTVRRLSWMAVADWNLVVLDEAQAIKNAAAQQSRA
                     VKSLRSRGRIVLTGTPIENRLGDLWSIFDFLNPGLLGSARQFTEMTRRLAERPHNAFG
                     PLRDLVRPYILRRLKTDPRIISDLPDKTEVTAYCLLSRVQAALYEQAVRDLKRRLAET
                     TEGIDRRGLVLASLLRLKQICNHPSHWLGDQAWGPADSGKWARLAEIVDVVSARQEKM
                     LVFTQFREMTGPLAAYLASLWGRPGLVLHGGFREPQRVSGIVA"
     gene            complement(2351..3331)
                     /locus_tag="PKK95_14275"
     CDS             complement(2351..3331)
                     /locus_tag="PKK95_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HOC19435.1"
                     /translation="MLACRVFREQEATAARVVEMSPDDLTPGEVVIRAEWSGVNFKDA
                     LGVTGRAPIYKRLPLNAGIDVSGRVESSADPRFRPGDAVLVNGMGLGESGDGGFAQVV
                     RVPADWVVPFPAGLDLREAMVLGTAGFTAALALHRMEANGQDPGKGAIVVTGATGGVG
                     SAAVSMLAGRGYRVVAVTGRPGHEGYLRELGAHEVVTPEGLRIGSRPLESARFGGAID
                     NVGGALLAGLLRHVVPWGNVAAIGNAGGPGFEGNVFPFILRGVSLLGASSANCPMGLR
                     TGIWNRLGADLRPPALARIASRTVRLPEVIGACEALMSRSALGRVLVDCR"
     gene            complement(3361..3747)
                     /locus_tag="PKK95_14280"
     CDS             complement(3361..3747)
                     /locus_tag="PKK95_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19436.1"
                     /translation="MDQTPDDSGLERLRAKASSVGVTAARHHIFLCCEQSKPKCCDLE
                     RGIAAWDYLKGRLKELGLSEEGGVLRTRANCLRICADGPIAVVYPEGVWYRACDPPVL
                     ERIIQEHLIGGRPVEDYVIVRQELGG"
     gene            3908..>4383
                     /locus_tag="PKK95_14285"
     CDS             3908..>4383
                     /locus_tag="PKK95_14285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC19437.1"
                     /translation="MKRPDIPPAVLALSGAHMVSDIFSNIYAPLLPLFIPKLGLSLAG
                     AGALAMAIQLSGSVAQLFFGPLADRWRPRVLAVAGPVVTVSCLGFAGWAVSPLMLGAI
                     LVVGNLGSAAFHPTAAAIVNRVGGERRGTAMSVHVTGGAIGNAVAPLVFAPYAARFG"
BASE COUNT          612 a         1638 c         1515 g          618 t
ORIGIN      
        1 tccgcaacaa taccggaaac acgttgcggc tcgcggaagc cgccgtgcag cacgaggccg
       61 ggccggcccc agaggctggc gaggtaggcg gcgagcgggc cggtcatctc gcggaactgc
      121 gtgaagacca gcatcttctc ctgccgcgcc gagaccacgt ccacgatctc ggccagccgt
      181 gcccacttgc cgctgtcggc cggtccccag gcctggtcgc ccagccagtg cgacgggtgg
      241 ttgcagatct gcttgaggcg caggagcgac gcgagcacca ggccgcgccg gtcgatcccc
      301 tcggtcgtct cggccagccg ccgcttcagg tcgcgcacgg cctgctcgta gagggcggcc
      361 tgcacgcggc tcagaagaca gtacgcggtc acctcggtct tgtcgggaag gtccgagatg
      421 atccgcgggt cggtcttcag ccggcggagg atgtaggggc gcacgaggtc gcgcagcggg
      481 ccgaaggcgt tgtggggacg ctcggccagc cgcctggtca tctcggtgaa ctggcgcgcc
      541 gacccgagca gccccgggtt caggaagtcg aagatcgacc agaggtcgcc caggcggttc
      601 tcgatcggcg tgccggtcag cacgatccgc ccccgcgacc gcagcgactt caccgcccgg
      661 ctctgctgcg ccgcggcgtt cttgatcgcc tgcgcctcgt ccaggacgac gaggttccag
      721 tcggcgaccg ccatccaaga gagacggcgc acggtgccgt aggtggtgag gacgatgtcg
      781 gcccgggcga gccgctccgg atcgggcgat ttcagttcgg ccgcggggac cgccgagggg
      841 tgcgcgacga acgtggagag ggttggggtg aacctggcga tctccgccgc ccagttcggc
      901 agcaccgatg ccggcgccac cagcagcgtt ccgccgcagc cggtcttccg ccgctcgatc
      961 gccgccagca gcgccagcac ctgcaccgtc ttgccaagac ccatgtcgtc ggccaggcac
     1021 gccccgaggc caagcgacga caggaagtgc agccaccgaa cgcccacctg ctggtagggg
     1081 cgcagcgatc cctggaaagc ctccccgggg tccacgcccc cgagcgcgtc gggcgatcgc
     1141 aggcgctgca ggacgccggc cagccacggt cccgcctcca ctcccgccca cgcgcggtcc
     1201 tcctcgtcgg ccgcctcctg cagccggggg tcggccccgg ccaccagcct catcgcctcg
     1261 acgaagctga gcccctcgcg tgccgccgcc tgctcgatgc ggctgaagcg gtcgatcatc
     1321 ccgccgagac gctcgcgatc gatctccacc caccggccgc gaagcagcgc cagtccctcg
     1381 gtggcccgca gcagcgatcg gatttcctcg gcagtcagcc tttcgccgtc gagcgtgacc
     1441 tctacgtgga aatcgagcag cgactcgagg ccgaggccgc cggcagccgg ctttgcgccc
     1501 acccgtgcga cgacggtcgg gcggggaggg cgaccggcct tccagtgcgc cggcacgcgc
     1561 acgatcacgc cggcgtcttc gagcacgtgc acgtcgcgga ggaagttcag cgcctcggcc
     1621 ggcgtccagc gcagggggtg gaagacctcg ccgctctcga ccatggcctt cagccaggcg
     1681 cagcgctcgg cggcccgctg cacgggaacc aggagcgaca gcagcgctcc ccggttggcc
     1741 gccccgccgt actcctcgag cgcgcgcccg agcgggacgt gccgcggctt gccctgcgac
     1801 gacaaacctt gcgtgtaggt ggcgaggaag gcgaacggcg cgtccgggtc gcggcggttc
     1861 tcggccaggt ggaagtgcac ccgcccgacg gtattccagg cgccgttgaa cgtgcggagg
     1921 acgtcctgga ccgcggcgcc gctctcggcg aggcgggtcg cgaaggcggc gtcgaggtcg
     1981 gtccagagcg cccgcagcga cgcggcggtg acgtactccg ccccggccat cggcggcacc
     2041 gcggcggccg tcgtctcgag cacctcgtcg ggcggcggcg cgacgtgcgg cgactcgggc
     2101 tgcgcgcaga gcgcgctcac gaaccgtgcg gcgaagtcgc gccagtagtc ccacgcaggg
     2161 ggcagcgggg tggtcacctc gccggcgccg agctggagca ggccctcccc gtggccgcgt
     2221 tcgaaggctg cggcgagccg cggcgctacc tgcgaggtcg ggtcccgaat ctcggcaaga
     2281 gccgccggtt cgagatccga ccccgatgcc agcagctggc cgcgcggcgt gaagaccggc
     2341 tcgagcggca tcagcggcag tccaccagca cccggccgag tgcgctccgc gacatgagcg
     2401 cctcgcaggc cccgatgacc tcgggcagcc gcaccgtccg gctggcgatt ctggcgagcg
     2461 cgggcggccg caagtcggcg ccgagccggt tccagatccc ggtcctcaag cccatcgggc
     2521 agttcgccga gctggccccg agcaggctca cgccgcggag gatgaagggg aagacgttcc
     2581 cctcgaagcc ggggccgccg gcattgccga tcgccgctac gttcccccat ggcacgacgt
     2641 gcctcaggag tccggccagg agcgctccgc cgacgttgtc gatcgcaccg ccgaaccgcg
     2701 ccgactcgag cgggcggctc ccgatccgca agccctccgg ggtcaccacc tcgtgcgctc
     2761 ccagctcgcg caggtagccc tcgtgcccgg gacggcccgt caccgccacc acccggtaac
     2821 ctcgccccgc cagcatcgac accgccgccg atccgacgcc cccggttgcg ccggtcacga
     2881 cgatcgcgcc cttgcctggg tcctggccgt tggcttccat ccggtgaagc gccagcgccg
     2941 ccgtgaagcc ggccgtgccg agcaccatcg cctcgcggag gtcgaggccg gcgggaaacg
     3001 gcaccaccca gtcggccggc acccgcacga cctgggcgaa cccgccgtcg ccgctctcgc
     3061 ccagccccat gccgttcacc agcacggcgt cgccgggtcg aaagcgcggg tctgcagacg
     3121 actcgacgcg gcccgagacg tcgatcccgg cattgagcgg caggcgcttg tagatcggcg
     3181 cccggcccgt cacaccgagg gcatccttga agttgacgcc cgaccactcg gcgcggatga
     3241 cgacctcgcc gggggtgaga tcgtccggcg acatctcgac cacccgcgcg gcggtcgcct
     3301 cctgttcccg gaacacgcgg cacgcgagca tgcgatcgtt ctcctactct ccctcttttc
     3361 ctaccctccg agttcctgcc ggacgatgac gtagtcctcc accgggcggc cgccgatgag
     3421 gtgctcctgg atgatccgct cgagcacagg cgggtcgcag gcgcggtacc agacgccctc
     3481 ggggtagacg accgcgatcg ggccgtccgc gcagatgcgc aggcagttgg ccctcgtgcg
     3541 cagcacgccg ccctcctccg agagccccag ctccttcagc cgccccttga ggtagtccca
     3601 cgcggcgatg ccccgctcga ggtcgcagca cttgggcttg ctctgctcgc agcagaggaa
     3661 gatgtgatgg cgggcggcgg tgacgccgac agacgacgcc tttgcgcgga ggcgttcgag
     3721 ccccgagtcg tcaggggttt ggtccatggg ggaagtattc cacaggatcg cggcccaccc
     3781 cgattcccca ctcccgattc ccgactgccg aatcctggat cccgactccc gattccccga
     3841 atcccgaatc ccgaatcccg attcctgtat gctgtgagat tgtgcctgtt cccgaggtgc
     3901 cctgcaggtg aagcgtcccg acatcccgcc ggccgtcctc gcgctctccg gcgcgcacat
     3961 ggtcagcgac atcttctcga acatctacgc gccgctgctg ccgctgttca tcccgaagct
     4021 cggcctgtcg cttgccggcg ccggcgcgct ggcgatggcg atccagctct cgggctcggt
     4081 tgcgcagctg ttcttcggcc cgctggccga tcgctggcgg ccgcgggtgc tcgcggtggc
     4141 cggccccgtc gtgacggtga gctgtctcgg gttcgccggc tgggcggtca gcccgctcat
     4201 gctcggcgcg atcctcgtgg tggggaacct cggcagcgcg gccttccatc ccaccgccgc
     4261 ggccatcgtg aaccgtgtcg gtggcgagcg gcgcggaacg gcgatgtcgg tgcacgtcac
     4321 cggcggggcg attgggaacg cggtcgcgcc gctggtcttc gccccctacg cggcgcgctt
     4381 cgg
//
LOCUS       DAOPQX010000326         4380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21738_length_4380_cov_5.045087, whole genome shotgun sequence.
ACCESSION   DAOPQX010000326 DAOPQX010000000
VERSION     DAOPQX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21738_length_4380_cov_5.045087"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PKK95_14290"
     CDS             complement(<1..842)
                     /locus_tag="PKK95_14290"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19438.1"
                     /translation="MPSPSPSTIVALATPPGRAGLAVVRLSGPDAFRIVSSLLAEPQP
                     LEPRRAIVARIQATVPAERPEEVREGELRSQLREPVQGLPGRDRGEVHGSRTEGVRVT
                     EGGAAAARWAIDQAVVLPFRAPASYTGEDVVELSLHGSMAVVAEVIAAAVRAGAVMAG
                     PGEFTLRAFLNGRLDLAQAEAVRDLVDAATPLQARVAFDQLTGTLSGRIAGIERELFS
                     LAARLEASIDFPDEGYRFVAGGEARAALERAAAMVDELLATAARGRVIREGATVAIGG
                     RPNVG"
     gene            complement(1020..1538)
                     /locus_tag="PKK95_14295"
     CDS             complement(1020..1538)
                     /locus_tag="PKK95_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOC19439.1"
                     /translation="MSPERTDNRELKTTRVTVDDLDLNSRLLDFVQSVTDAMGLDVTG
                     TVEDTPDGPRVNLEGEDGSVLVRHKGEALQALQHLASTVFRPELPDHKRLVVDCLDFR
                     RDKDVELRQMALFLAEKASRTGTEQRLGPLNAYERRIVHMAVAEQGLADTESIGDAAV
                     KVVIITAKPGRK"
     gene            complement(1569..3353)
                     /gene="yidC"
                     /locus_tag="PKK95_14300"
     CDS             complement(1569..3353)
                     /gene="yidC"
                     /locus_tag="PKK95_14300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOC19440.1"
                     /translation="MEKRVLLAVFLSFLVLFGYQTLVKPKGPAPRRTPPPATDSTQAA
                     PAAGEAAPGSMAPGAPGSAAEPGAAPTETPASPAAEPLVADASERDIIVETALYRVVI
                     STRGARVTSWTLKHYQDLQTKQPLDLVPSGLPGRPVRPFELRVDDETANARLRGALFR
                     PDEGARDGRVDATRAAQTVTFEYRDAAGLEARKSFTFEPNTYLVGFDADVSVGGRPLN
                     PTILWGPGLGDLRPGGSSYEQKPQGMVFRGRDVDRINAGEVADGQALQEGTFAAAGVD
                     DLYFISLALPVRPVRVQYEAVVVPGPGGDPKQSLPYMAWSIRQAQSPQGLRFYVGPKD
                     FDTLKSVDPGLVKAINFGILDWLAVPLLDALNWIYGYVGNYGWAILILTVLINLLMFP
                     LRHKSMVSMRKMQAIQPEVKAIQERYKGLKTTDPERQKMNTELMNLYRQRGVNPASGC
                     VPMLLTMPVLFAFYSLLSQAIEMRGAPFILWITDLSKHDPYYVTPLMMGASQVWQQRM
                     TPAGGDPMQQKMMMMMPVIFTFMFLWAPSGLVLYWFTSNVLAIGQQYLTNRLAGPPPK
                     LEKATPVAGSALATPSPAPPMGPRRKKK"
     gene            complement(3358..3615)
                     /gene="yidD"
                     /locus_tag="PKK95_14305"
     CDS             complement(3358..3615)
                     /gene="yidD"
                     /locus_tag="PKK95_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOC19441.1"
                     /translation="MSSPSLAARLLLALVGAYRVLLAPLVGGACRYTPSCSQYMTEAV
                     RQHGALRGGWLGVRRLARCHPFGGHGFDPVPAPYRPGVKVR"
     gene            complement(3713..4093)
                     /gene="rnpA"
                     /locus_tag="PKK95_14310"
     CDS             complement(3713..4093)
                     /gene="rnpA"
                     /locus_tag="PKK95_14310"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOC19442.1"
                     /translation="MRRRPEFQRVYDGGQRLSLRLMTVILMPNGLPQSRLGVSATKKL
                     GGSVVRNRAKRVVREVFRQADVPGGLDLVVIPRPALLEAEYQAVAAEFQYALRRAGRR
                     AGGREAGRPRPPKERGEGGNRTPA"
     gene            complement(4143..4280)
                     /gene="rpmH"
                     /locus_tag="PKK95_14315"
     CDS             complement(4143..4280)
                     /gene="rpmH"
                     /locus_tag="PKK95_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOC19443.1"
                     /translation="MKRTFQPNNRRRHKTHGFLVRMSTKGGRLVLKRRRAKGRKRLAL
                     N"
BASE COUNT          641 a         1552 c         1523 g          664 t
ORIGIN      
        1 ccgacgttgg gccgcccgcc aatcgcaacg gtcgcacctt cccggatcac acgcccccgg
       61 gccgcggtcg ccagcagttc atcgaccatc gccgccgccc gttccagcgc cgcccgcgcc
      121 tcgcctccgg ccacgaaccg gtacccctcg tctgggaaat cgatcgacgc ttcgaggcgc
      181 gcggccagcg agaacagctc ccgctcgatc ccggcaattc gcccgctgag cgtcccggtc
      241 agctggtcga aggccacccg ggcctggagc ggtgtggccg cgtccaccag gtcgcgcacg
      301 gcctcggcct gcgccaggtc gaggcgcccg ttcaggaacg cccggagcgt gaactcgccc
      361 ggccccgcca tgaccgcccc ggccctcacc gccgcggcaa tcacctcggc caccacggcc
      421 atgctgccgt gcaggctcaa ctcgacgaca tcctctcccg tgtacgaggc aggcgcgcgg
      481 aacggaagga ccaccgcctg gtcgatcgcc cagcgagccg ccgcagcgcc gccctccgtc
      541 actcggacgc cttcggtgcg ggacccgtgc acctcgcccc ggtcgcggcc cggcaaccct
      601 tgcaccggct cccggagctg gctccggagt tcgccctccc ggacctcctc agggcgctcc
      661 gcgggaacag tggcctggat gcgggcaaca atggcgcggc gcggctcgag cggctgcggc
      721 tcggccagca gggaggaaac gatgcggaac gcgtcggggc cgctcaggcg caccacggca
      781 agcccggcgc gccccggggg tgtggcgagc gcgacgatcg tggaggggga gggggacggc
      841 acttcagtcc acgaagtccc gccttcgctc cactcgatca cgaggagcta cggcgaggca
      901 agcacgaggt cgcgaagtca cgaagtcgcg aagtccagtc gacgaagtcc cgccttcgct
      961 ccacctgatc gcgaggagct acggcgggca ggctccggct ccggcgaggc aagccgaaac
     1021 tattttcgtc ccggtttcgc ggtgatgatg accaccttga cggcggcgtc gccgatgctc
     1081 tcggtgtcgg ccagcccttg ctcggccacc gccatgtgga cgatgcgccg ctcgtaggcg
     1141 ttgagggggc cgagccgctg ctcggtgccc gtccggctgg ccttctcggc caggaacagc
     1201 gccatctggc gcagttccac gtccttgtcc cggcggaagt cgaggcagtc caccacgagg
     1261 cgtttgtggt cgggcagctc ggggcggaac acggtcgagg cgaggtgctg cagcgcctgc
     1321 agcgcctcac ccttgtggcg caccagtacg ctgccgtcct ccccctcgag gttgacgcgg
     1381 gggccatcgg gcgtgtcttc gacggtgccc gtcacgtcga gtcccatcgc gtcggtcacg
     1441 ctctgcacga agtcgagcaa gcggctgttg aggtcgaggt cgtccacggt gactcttgtc
     1501 gttttcagtt cccggttgtc ggttcgctca gggctcacgg cgccctccgc cttccgatct
     1561 acgagccgct acttcttctt ccgccgcggc cccatgggcg gtgcgggcga aggggtggcc
     1621 agcgcgctcc cggcaaccgg cgtcgccttc tccagcttgg gcggcggccc ggccaggcgg
     1681 ttggtcaggt actgctgccc gatcgcgagc acgttgctgg tgaaccagta gagcacgagg
     1741 ccgctcggcg cccacaggaa catgaaggtg aagatgacgg gcatcatcat catcatcttc
     1801 tgctgcatcg ggtcgccgcc ggccggcgtc atccgctgct gccacacctg gcttgcgccc
     1861 atcatcagcg gcgtcacgta gtacgggtcg tgcttcgaca ggtcggtgat ccaaaggatg
     1921 aacggggcgc cgcgcatctc gatcgcctgc gagagcagcg agtagaacgc gaacagcacc
     1981 ggcatcgtca gcagcatcgg cacgcagccg ctggccgggt tcacgccccg ctggcgatag
     2041 aggttcatca gctccgtgtt catcttctgc cgctcggggt cggtcgtctt caaccccttg
     2101 tagcgttcct ggatcgcctt cacctcgggc tgaatcgcct gcatcttccg catcgacacc
     2161 atgctcttgt ggcggagcgg gaacatcagc aggttgatga ggacggtcag gatgaggatc
     2221 gcccagccgt agttgccgac gtagccgtag atccagttga gcgcgtcgag cagcgggacg
     2281 gcaagccagt cgaggatgcc gaagttgatg gccttgacca gcccggggtc gaccgacttg
     2341 agcgtgtcga agtccttcgg gccgacgtag aaccgcagcc cctggggcga ctgcgcctgc
     2401 cggatgctcc aggccatgta gggcagcgac tgcttggggt cgccgcccgg cccgggcacc
     2461 acgaccgcct cgtactgcac gcgcaccggc cgcaccggca gcgcgaggct gatgaagtag
     2521 aggtcgtcca cgccggcggc ggcgaacgtc ccctcctgca gcgcctggcc gtcggccacc
     2581 tcgccggcgt tgatgcggtc cacgtcgcgg ccccggaaca ccatcccctg cggcttctgc
     2641 tcgtagctgc tgccgccggg gcgcaggtcg ccgaggccgg gcccccacag gattgtcggg
     2701 ttcagcggcc ggccgcccac gctcacgtcg gcgtcgaagc ccaccaggta ggtgttcggc
     2761 tcgaaggtga agctcttccg cgcctcgagg ccggccgcgt cgcggtactc gaaggtcacc
     2821 gtctgcgccg ccctggtcgc atccacccgg ccgtcgcgcg ccccctcgtc gggccggaac
     2881 agcgcccccc tcagcctcgc gttcgcggtc tcgtcgtcca cgcgcagctc gaagggccgg
     2941 accggccgtc ccggcaggcc actcggcacg aggtcgagcg gctgtttggt ctgaaggtcc
     3001 tggtagtgct tcagggtcca gctcgtgacc cttgcgccgc gtgtgctgat gaccacccgg
     3061 tacagcgccg tctcgacgat gatgtcgcgc tcggaggcgt ctgcgacaag aggctcggcg
     3121 gcgggggagg caggcgtctc ggtgggggcg gctcccggct cggccgcact tcccggggct
     3181 cccggcgcca tcgagcccgg cgcggcctcg ccggcggcgg gggcggcctg cgtggaatcg
     3241 gtggccgggg gcggtgtgcg ccgcggcgcg ggtcccttcg gcttgaccag cgtctggtag
     3301 ccgaacagga cgaggaacga gagaaagacc gcgaggagta cgcgcttttc catgaagcta
     3361 gcgaaccttc acgccagggc gatagggagc cggcacgggg tcaaagccgt ggccgccaaa
     3421 cgggtggcac cgcgcgagcc gccgcacacc cagccagccg ccgcgaaggg ctccgtgctg
     3481 cctcaccgct tcggtcatgt actgggagca cgacggggtg taccggcacg cgccgcccac
     3541 cagtggagcc aacagcaccc tgtaggcgcc caccagcgcc agcagcaggc gtgcagccag
     3601 gctcggggag ctcatgccag ccgccggcag gacgggttga aaggagacgc ctcggaacgg
     3661 ggcgcggaag acgggcggtt tcgaagcatc accggacagc tcattctacg actcacgccg
     3721 gcgttcggtt gccaccctct ccgcgctcct tcggcggccg gggccgcccg gcctctctgc
     3781 ccccggcgcg ccggccggcg cggcgcaggg cgtactggaa ctccgcggcg acggcctggt
     3841 actcggcctc gagcagggcg gggcggggaa tgacgacgag gtcgaggccg cccgggacgt
     3901 cagcctgccg gaacacctcg cgaacgacgc gcttggcgcg gttcctgacc accgagccgc
     3961 ccagcttctt cgtggccgag acgcccaggc ggctctgggg gaggccgttg ggcatcagga
     4021 tgaccgtcat cagcctcagg ctgagacgct gcccgccgtc gtacacgcgc tggaactcgg
     4081 ggcgccggcg aatccggcgc tcggggccga atgcatgagc tcgcgcgtcc agctcccagc
     4141 tcctagttca acgccaggcg cttccgcccc ttggcgcggc ggcgcttcag gaccaggcgg
     4201 ccgcccttgg tggacatgcg gacgaggaac ccgtgcgtct tgtggcgacg ccggttgttg
     4261 ggctgaaacg tgcgcttcat ggtcgttccc gggagatgcg cgaggcgtct cgcgcggcag
     4321 tgtcaggaca gcgagaatct tcaatggtag cggtgtggga gcggcagtgt caacgaaaag
//
LOCUS       DAOPQX010000327         4366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21834_length_4366_cov_4.130596, whole genome shotgun sequence.
ACCESSION   DAOPQX010000327 DAOPQX010000000
VERSION     DAOPQX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21834_length_4366_cov_4.130596"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>448)
                     /locus_tag="PKK95_14320"
     CDS             complement(<1..>448)
                     /locus_tag="PKK95_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19444.1"
                     /translation="PPSTIGLTVVLALSAAAPADVAQAPAACIAGLIPLERIVAGNPD
                     DLFAGADCRQAAIRCGAFDRSIDFFKRLARKHPRSASVRLNLALAYVDKIPSAGDLRQ
                     ALLGRDAIGELGRANEIAPTWIGHYTRGFIYLNYPRAFGGGPKAIAE"
     assembly_gap    449..458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<459..2954)
                     /locus_tag="PKK95_14325"
     CDS             complement(<459..2954)
                     /locus_tag="PKK95_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC19445.1"
                     /translation="MPPARGRPRGCASGVRSLPGKALLIGVVAALLGGAGPASAQGSA
                     TLQGTVTAAGGLVRLPGVIVVVTAEAGTPPREVVTDGEGRFRLEDLAQGPAAVSVSLE
                     GFERFESRVVLAGGRGIELAVDLAPAGLTERVDVVQRAEDAAMATSLGTKDTLDGSFI
                     RRSAVTDSSLQGALLALPGVVLTPEGLSIRGGRPAQSAVQIGAATIADPATGIAPLRL
                     PVDAVASVDVMPNPYAVEFGRFSSGITLINTRQGGNRWRLSLNNFDPSFRTRRGRPLD
                     VLGIGSFAPRLGFGGPLAGDRVRVAGSFQYRHTLGEVQSRPQDEVRLQEYLSGLIRLD
                     ATISPRHSVSLVLSEFPERRTLVNLDTFTPPEATFDQRQRISNAVVTHSAVLSGNAAL
                     ASSIQWSRYDARVWPKGGAPLRIAPGGRSGSHFTEGRRNTEAFQWTEALSVYAKGWTG
                     EHLLKAGLDVLGARFEGSSRGGAIEIVRADGTLAREVRFLGPAEQGAATTDVALFAQN
                     RWRPVRAVAVEAGMRLDRDGVLGRTNLTPRVGAAWSFGAGGSFVLRGGAGLFYERPPS
                     SARAFGQLEERVVATFGPDGTTLAGPPVHFAHRADAGLQTPHSFTWSLDLSKQVNRWF
                     TWRIGHLQRKGRDELIVDALVDGAAGTLLLSSGGRSRYRETEASLGVVPGRDSELRVS
                     YVHSSAEGDLNAFSSLFGAGGDPLVRPNEWGPLPGDLRRRLILRGHALPRGKWFLETA
                     VDWRSGFPYSALDERQDYAGPRNRAGRFPSSAVVDIAVERRIRVLSWRPWIGVRLKNA
                     FNAFAPLDVQQNAASPLFGAFYDSVPRRAHFIVR"
     gene            complement(2882..3997)
                     /gene="hisC"
                     /locus_tag="PKK95_14330"
     CDS             complement(2882..3997)
                     /gene="hisC"
                     /locus_tag="PKK95_14330"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOC19446.1"
                     /translation="MGNGRRISVRPTIARLRAYRPGDGASGPGASNGRVRLDWNEGSR
                     PPSPAVMARLRAFLEGGRLNRYPDPTAARLRRAIAAYARRPVSGVQVFNGSDAALDCL
                     VRTFVAPGDHVVISAPCYDQFRVFAEAAGAAVQAVRGKDPFEADVQPLASSITPATRL
                     VYLCNPNNPTGRTHSRADLRRLLAALGDGLLVVDEAYYEFYGRTTAPLIDADPRVTVV
                     RSFSKAFGLAGLRCGYLLAAPEVVAAVNRLRNGKDVNALAQVAAIAALADIPYMRESV
                     TEMRRNRALLAGALRSAGWRVVVTPANFILLECASPSAVAARLERRGIFVRDRSDLPQ
                     LERYVRVTVGTADECRRLVAALEGVRPESDPCQAKPC"
     gene            complement(3999..>4366)
                     /locus_tag="PKK95_14335"
     CDS             complement(3999..>4366)
                     /locus_tag="PKK95_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19447.1"
                     /translation="GVTAGVARAGYPAWLLAAGLVTIAMDVLTVSVMYWTLAARGDAT
                     LLAFEADLDRPAFGRGLLPDLARRFQPFVKRDAYAFLFMLAAVAGAPWVPLVTTPPAL
                     AFTLAGFARIVVVQRESEA"
BASE COUNT          656 a         1589 c         1571 g          540 t
ORIGIN      
        1 gctcggcgat cgccttcggg cccccgccga acgcgcgggg gtagttcagg tagatgaagc
       61 cgcgcgtgta gtgcccgatc cacgtgggcg cgatctcgtt cgcccggcca agctcgccga
      121 tcgcgtcacg gccgagcagc gcctggcgca ggtcgcccgc cgacgggatc ttgtccacgt
      181 aggcgagcgc gaggttcagc cgcacgctcg ccgaccgcgg gtgcttgcgc gcgagccgtt
      241 tgaagaagtc aatcgaacgg tcgaaggcgc cgcatcggat tgccgcctgc cggcagtctg
      301 ccccggcgaa gagatcgtcg gggttcccgg cgacgatgcg ttcgaggggg atcagccccg
      361 cgatgcaggc ggcaggcgcc tgcgccacat ccgccggcgc ggcagcggac agggcgagga
      421 cgacggtgag accgatcgtc gagggcggnn nnnnnnnncc ggactatgaa gtgtgcgcgg
      481 cggggcaccg agtcgtagaa cgccccgaac aagggcgagg cggcgttctg ctgcacgtcc
      541 aagggggcga acgcgttgaa ggcgttcttc agccggacgc cgatccaggg gcgccacgag
      601 agcacccgga tgcggcgctc gaccgcgatg tccacgaccg cgctcgacgg gaaccgcccg
      661 gcccggttgc gcggcccggc gtagtcctgc cgctcgtcga gggccgagta cgggaagccg
      721 ctgcgccagt ccacggcggt ttcgaggaac catttcccgc gcggcagcgc gtgcccccgg
      781 aggatgagcc ggcggcgcag gtcgcccggc agcggccccc actcgttcgg ccgcaccaac
      841 gggtccccac cggcaccgaa caacgacgag aaggcgttca ggtcgccctc ggccgacgag
      901 tgcacgtacg aaacgcgaag ctcgctgtcc cgccccggga cgacgccgag gctcgcctcg
      961 gtctcgcggt accgcgagcg gccgcccgac gacaagagca gggttcccgc cgctccgtcg
     1021 acgagggcgt ccacgatcag ctcgtcgcgt cccttccgct gcaggtgccc gatccgccag
     1081 gtgaaccagc ggttcacctg cttcgacagg tcgaggctcc aggtgaagct gtgcggagtc
     1141 tggagaccgg cgtcggcgcg gtgggcgaag tgcacgggcg ggcccgccag agtcgtcccg
     1201 tcggggccga aggtcgcgac cacgcgctcc tccagctgcc cgaacgcccg cgcgctcgaa
     1261 ggcggccgct cgtagaacag gccggcgccg ccgcgaagga cgaagctccc gccggcgccg
     1321 aacgaccagg cggcgcccac ccgcggcgtc aggttcgttc gtcccaacac cccatcgcgg
     1381 tcgaggcgca tcccggcctc gacggcgacc gccctcaccg ggcgccaccg gttctgggcg
     1441 aacagcgcga cgtcggttgt cgccgcgccc tgctcggctg gcccgaggaa ccgcacctcg
     1501 cgcgcgagcg tgccgtcggc ccgcacgatc tcgatcgccc ccccgcgact gctgccctcg
     1561 aaccgcgcgc ccagcacgtc gagccccgcc ttgagcaggt gctcgcccgt ccaccccttc
     1621 gcgtacaccg acagcgcctc ggtccactgg aacgcctcgg tattgcgccg tccctcggtg
     1681 aagtggcttc ccgacctccc gccgggggcg atccgcagcg gcgccccccc cttgggccag
     1741 acccgcgcgt cgtagcggct ccactgaatg gagctggcga gcgccgcgtt gcccgagagc
     1801 acggccgagt gcgtcacgac cgcgttcgag atccgctggc gctggtcgaa ggtcgcctcg
     1861 gggggcgtga aggtgtcgag gttgaccagc gtccgccgct ccgggaactc gctcagcacg
     1921 aggctcaccg agtggcgggg cgagatggtc gcgtcgaggc ggatgaggcc gctcaggtac
     1981 tcctgcaggc gcacctcgtc ctgcggccgg ctctggacct cgccgagcgt gtgtcggtac
     2041 tggaagctgc ccgccacgcg cacccgatcg ccggcgagcg gccccccgaa ccccagccgg
     2101 ggtgcgaacg agccgatgcc cagcacgtcg agcggccggc ccctcctggt gcggaaggag
     2161 gggtcgaagt tgttcagcga gagccgccag cgattgcccc cctgcctcgt gttgatcagc
     2221 gtgatgccag atgagaagcg gccgaactcc accgcgtacg ggttgggcat cacgtccacc
     2281 gacgccaccg cgtccaccgg cagccgcagc ggcgcgatgc ccgtggccgg gtccgcgatc
     2341 gtcgccgcgc caatctgcac cgcgctctgc gcggggcggc ctccacggat gctcaatcct
     2401 tccggtgtca ggacgacgcc cggcagcgcc aggagagctc cttgaagcga ggagtcggtg
     2461 acggcggagc gacggatgaa gctgccgtcg agggtgtcct tcgtgccgag cgaggtggcc
     2521 atcgcggcgt cctcggcccg ctggacgacg tcgacccgct cggtcaggcc agccggcgcc
     2581 aggtccacgg ccagttcgat gccccggccg ccggccagca cgacgcggct ttcgaagcgc
     2641 tcgaacccct cgaggctgac gctgaccgcc gccggcccct gggcgaggtc ttccagccgg
     2701 aaccgtccct caccgtcggt gacgacctcg cgcgggggag tccccgcttc ggcagtcacg
     2761 acgacgatga cacccgggag acggaccaac ccgccagccg cggtgacagt gccctggagt
     2821 gttgccgacc cttgggcgga cgccggcccc gccccaccga ggagggccgc gacgacgccg
     2881 atcagcaggg ctttgcctgg caaggatctg actccggacg cacaccctcg agggcggcca
     2941 cgagccggcg gcattcgtct gcggtgccga ccgtcaccct gacgtagcgt tcgagctgcg
     3001 ggaggtcgct gcggtcgcgc acgaagatgc ctcgcctttc gagcctggcg gcgacggcgg
     3061 agggcgaggc acactcgagc aggatgaagt tggccggcgt caccacgacc cgccagccgg
     3121 ccgagcgcag cgccccggca aggagcgccc ggttccgccg catctcggtt accgactccc
     3181 gcatgtacgg gatatcggcc agcgcggcaa tcgccgccac ctgggcgagc gcgttcacgt
     3241 ccttgccgtt gcgcagccgg ttcacggctg cgacgacctc cggcgccgcc agcaggtagc
     3301 cgcaccgcag cccggccagg ccgaaggcct tcgagaagga gcggacgacg gtcacccgcg
     3361 ggtcggcgtc gatgagcggg gccgtcgtgc gcccgtagaa ctcgtagtag gcctcgtcca
     3421 ccaccagcaa cccgtcgccc agagcggcga gcagccggcg caggtcggcg cgcgaatgag
     3481 ttcggcccgt cgggttgttc gggttgcaga ggtagaccag ccgcgtcgcc ggggtgatgc
     3541 tcgaggccag cggctgcacg tcggcctcga acgggtcctt gccgcgcacc gcctgcaccg
     3601 ccgcgccggc ggcctcggcg aacacgcgaa actggtcgta gcagggagcc gagatcacca
     3661 cgtgatcgcc cggggcgacg aacgtccgga cgaggcagtc gagcgcggcg tccgacccgt
     3721 tgaacacctg gacgcccgag accggcctgc gcgcgtaggc ggcgatcgcg cggcgcagcc
     3781 gcgccgcggt ggggtcgggg taccggttga ggcgcccccc ctcgagaaag gcccgcagcc
     3841 gcgccatcac cgccggcgac ggcgggcgcg aaccctcgtt ccagtcgagc cgcacccttc
     3901 cgttggacgc gccgggaccg ctggccccgt cgcccggccg gtaggcgcgc agacgggcga
     3961 tggttggacg aacggatatg cgacggccgt ttcccatgtc aggcttcgct ctcccgctgg
     4021 acaacgacga tacgggcgaa gcccgcgagc gtgaaggcca gcgcgggcgg cgtggtcacc
     4081 agcgggaccc agggcgcgcc ggcgaccgca gccagcatga agaggaaagc gtacgcgtca
     4141 cgcttgacga acggttggaa ccgccgcgcg agatccggca gcaggccgcg cccgaaggca
     4201 ggacgatcga ggtcggcctc gaaggcgagc agcgtcgcgt cgccgcgcgc cgcgagggtc
     4261 cagtacatga cggagacggt gaggacgtcc atggcaatgg tgacgaggcc cgcggccagc
     4321 agccaggcgg ggtagcccgc ccgcgcgacg ccggcggtca cgccga
//
LOCUS       DAOPQX010000328         4351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21947_length_4351_cov_5.442737, whole genome shotgun sequence.
ACCESSION   DAOPQX010000328 DAOPQX010000000
VERSION     DAOPQX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21947_length_4351_cov_5.442737"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /locus_tag="PKK95_14340"
     CDS             complement(<1..898)
                     /locus_tag="PKK95_14340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOC19448.1"
                     /translation="MRAAWFPAAILAAFALALGLRLAAPGARPMHHDEANQAVKFGEL
                     LESGDYRYDPDDHHGPSLYYLTLPAAWLRGQHTLAALDEGTLRGVTAFFGAGLILLLP
                     LMAGGIGRPAAAVAALLIALSPALTYYSRFYIQESVFAFFAAGFLVALGRYAGAGDPR
                     WRRRWAVAAGVFAGLAYATKETSIIVGVAALVAWAAASALVRGGRPRVALGDLLVAAA
                     AALGVAIVFYSSFFRHPAGPLESFLAFATYFQRGVEPAGHAHPPGYYLRILGYTSSGG
                     LVWTEAAILALAVAGGAVAVRRA"
     gene            complement(1044..1712)
                     /locus_tag="PKK95_14345"
     CDS             complement(1044..1712)
                     /locus_tag="PKK95_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19449.1"
                     /translation="MKKRLILSACLVAFALAPLAARQAGDKEVLKLKLPKPMFIGTPT
                     NLKSPNLEVVTGKPRGPFYVPKGTTLLSLNKPVRSSDMQPVIGELDMVTDGEKEGGDG
                     YFIELGPGKQWLQIDLGKSHALHAILAWHYHSQARVYRDVIVQVSDDKDFLKGVTTIF
                     NNDHDNSAGLGVGKDKEYIEVAEGRLFDPKGAKGRYVRLYSAGNTSNDLNHYVEVEVY
                     GTPN"
     gene            complement(1808..3802)
                     /locus_tag="PKK95_14350"
     CDS             complement(1808..3802)
                     /locus_tag="PKK95_14350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC19450.1"
                     /translation="MNRPRSFILTCAAAALFLSAASLSGQEARTQADSASLDRLLANF
                     ATWDGGVASEARWALRDHVLAHRGDPAAREQIEAALVRYLSGKATGQAKMEAARHLRA
                     IAGEKSVGPLAALLRDQRTADYAIYVLQQVPGEAADQAMLAALSARRTALPIRKALVA
                     SLGQRRSAAAVPALSALVTDRDLAAAAITSLGRIGDPAARDALTAAWPKAAAPLRGVL
                     ASALLFSAEHALESNDPAAALRVYEMLAGDTALPAPIRGAALIGRIDASGPARTRVLV
                     EMLGGGDEAARGAAIARLREVITADTLEPVCALLPRLPEASKVQLLAALGTYPAERVR
                     PAVLQAARNETGAVRVAALKALGRTGSASEVAFLTELAASARGDEQAAARDALASLKG
                     PEADAAIVSALDAKPAPALEAELLQAVAARRIFVAKPAVAARLAAPASPRVLREALRT
                     LRSIGTPSDIPAVLDVLLAASQDDVIADAEQTVNALASKMAAVNRRAMPVRMRLRTEK
                     EPAARARLLAVLPLTADGSALAELRTAMRDPSPEVSDAAVRAIAAWPTASAYDDVMQV
                     ARESKDETHRLLAIQALIRMLTLDTSRRPDSVVGDLRAVAGMASRPEERRLVLGALAQ
                     FPGPEALEAARSFLSDASVKAEAEAAVESINKQMTRPWFR"
     gene            complement(3815..>4351)
                     /locus_tag="PKK95_14355"
     CDS             complement(3815..>4351)
                     /locus_tag="PKK95_14355"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19451.1"
                     /translation="VGHHLDIAHWGMDFERTGPVKISGTGEFPTSGIYNAARRYWVDA
                     VYADGTPFVLAGGYPEIQSGAKWIGDRGWVWVDRGGFEAEPAHLVNEVIGPNETRLYR
                     SRDHFQNFLDCVRSRALTIAPAEVAHRSASVGHLGVLAIETGRTIRWDPATETIQDDP
                     GAERLLSRAYRAPYQLPA"
BASE COUNT          629 a         1548 c         1576 g          598 t
ORIGIN      
        1 gtgcgcggcg caccgcgacg gcgccgcccg ccaccgccag cgccaggatc gccgcctcgg
       61 tccacacgag gccgcccgac gaggtgtagc caaggatgcg caagtagtag cccggggggt
      121 gcgcgtgccc ggccggctcg acgccccgct ggaagtacgt ggcgaaggcg aggaacgact
      181 cgagcggacc cgccgggtgc ctgaagaacg acgagtagaa cacgatcgcc acgcccagcg
      241 cggccgcggc cgcgacgagc aggtcgccga gcgccacgcg cggccgtccg ccgcgaacga
      301 gggcggatgc ggccgcccag gccacgagcg ccgcgacccc gacgatgatg gaggtttcct
      361 tcgtggcgta cgccaaaccg gcgaagacgc cggcagccac ggcccatcgc ctccgccacc
      421 gcggatcgcc ggcgccggcg tacctcccga gcgccacgag aaagccggcg gcgaagaagg
      481 cgaacaccga ttcctggatg tagaaacggc tgtagtaggt gagagccggc gagagggcga
      541 tcagcagtgc ggcaacggcc gcggctggac ggccgattcc acccgccatc agcggcagaa
      601 gcaggatgag cccggcgccg aagaatgcgg tgacgccgcg cagggtcccc tcgtccagcg
      661 cggcgagcgt gtgctggccg cgaagccacg cggcggggag cgtcaggtag tagaggctgg
      721 gaccgtgatg atcgtccggg tcgtagcggt agtcgcccga ctcgagcagc tcgccgaact
      781 tgaccgcctg gttcgcctcg tcgtggtgca tcggccgcgc gccgggcgcg gccagacgga
      841 ggccgagcgc cagcgcgaac gccgccagga tggcggccgg gaaccaggcc gccctcatct
      901 ggtcaccttc ggacgatcgc agcacggccc ccggcggcac cacacctcca gcaccggcac
      961 ccgaggcact ggcaccggca cccgaggcac cttcggctcc agcacctccg gcacctccag
     1021 cacccccggc accttcagca cgactagttc ggcgtcccgt acacctccac ctcgacgtag
     1081 tggttcaggt cgttggacgt gttgccggcg gagtacagcc tcacgtagcg gcccttggcg
     1141 cccttggggt cgaacagccg cccctcggcc acctcgatgt actccttgtc cttcccgacg
     1201 cccaggccgg ccgagttgtc gtggtcgttg ttgaagatgg tcgtgacgcc cttcaggaag
     1261 tccttgtcgt cggacacctg gacgatgacg tcgcggtaga cccgggcctg gctgtgatag
     1321 tgccaggcga gaatcgcgtg cagggcgtgg gacttgccga ggtcgatctg cagccactgc
     1381 ttcccggggc ccagctcgat gaagtagccg tcgccgccct ccttctcgcc gtcggtcacc
     1441 atgtcgagct cgccgatcac cggctgcatg tcgctggagc gcaccggctt gttgagcgag
     1501 agcagggtgg tccccttcgg cacgtagaac ggcccgcgcg gcttgccggt gacgacctcg
     1561 aggttcgggc tcttgaggtt ggtgggcgtg ccgatgaaca tcggcttcgg gagcttgagc
     1621 ttcagcacct ccttgtcccc cgcctggcgg gccgcaagcg gcgccagggc gaaggccacg
     1681 aggcaggcag acagtatcag tctcttcttc acggcaatcc tcatatgaaa gaacggcttg
     1741 cgatacgccc gggacgcgcc gggcgaggtc gcaggacctc acgcttcgct cagttcatga
     1801 tagggcatta gcggaaccag ggccgcgtca tctgcttgtt gatgctctcg acggccgcct
     1861 cggcctcggc cttgacgctg gcgtccgaga ggaagctcct ggccgcctcg agcgcctcgg
     1921 ggccggggaa ctgggcgagc gccccgagca ccaggcgcct ctcctcgggc ctcgacgcca
     1981 tgccggctac cgcgcgcagg tcgccgacga ccgagtcagg ccggcggctg gtgtcgagcg
     2041 tgagcatgcg gatgagcgcc tggatcgcga gcaggcggtg cgtctcgtcc ttcgactccc
     2101 ttgcgacctg catcacgtcg tcgtaggccg aagcggtagg ccaggctgcg atggcgcgga
     2161 cggcggcgtc cgacacctcg gggctggggt cacgcatcgc ggtcctcagc tcggccaagg
     2221 cgctgccgtc ggcggtgagc ggaagaacgg cgagcaggcg ggcccgggct gcgggttcct
     2281 tctcggtcct caggcgcatg cgaaccggca tggcgcgacg gttgacggcc gccatcttcg
     2341 acgcgagcgc gttgacggtc tgctcggcat cggcgatcac gtcgtcctgc gaggctgcga
     2401 gcagcacgtc gagcacggcc gggatgtcgg acggcgtgcc gatcgagcgc agcgttcgca
     2461 gtgcttcacg aaggacccgc ggcgacgcgg gcgcggccag ccgggcggcc accgccggct
     2521 tggccacgaa gatccgccgc gcggcgaccg cctgcagcag ctcggcctcg agggccggag
     2581 cgggcttggc atcgagagcc gagacgatcg cggcgtcggc ctcgggtccc ttcagcgagg
     2641 ccagggcgtc gcgggccgct gcctgctcgt cgcctcgcgc gctcgctgcc agctcggtga
     2701 ggaaggccac ctcagaggcc gacccggttc gccccagcgc cttcagcgcg gccacacgca
     2761 cggcccccgt ctcgtttcgt gccgcctgga gcacggccgg cctcacccgc tcggccgggt
     2821 aggtcccgag ggccgcgagc agctgcacct tggaggcctc tggcagccgc ggcagcagcg
     2881 cgcagaccgg ctcgagcgtg tcggccgtga tcacctcgcg gagccgggcg attgcagcgc
     2941 cgcgtgccgc ctcgtcgccg cctcccagca tctccacgag aacccgcgtc cgggcggggc
     3001 cggacgcgtc gatccgcccg atcagcgcgg cgccccggat gggagccggc agcgcggtat
     3061 cgccggcgag catctcgtag acgcggaggg ccgcggccgg gtcgttcgac tcgagcgcgt
     3121 gctccgccga gaacagcagc gcgctggcca gtacccctct cagcggcgcg gcggccttgg
     3181 gccacgcggc ggtgagcgcg tcgcgagcgg ccggatcgcc aatccgccca agcgaggtga
     3241 tggcggcagc ggcaaggtca cggtcggtca ccagggccga gagcgcgggg acggcagcgg
     3301 cgctccggcg ctgaccgagc gacgcgacga gcgccttccg gataggcagg gccgtcctgc
     3361 gcgcggagag tgcggcgagc atcgcctggt cggcagcctc gccgggcacc tgctgcagga
     3421 cgtagatcgc gtagtcggcg gtccgctgat cgcggagcag ggccgcgagc gggccgaccg
     3481 acttctcgcc ggcgatcgcc cgcaggtgcc gcgccgcctc catcttggcc tggccggtcg
     3541 ccttgcccga caagtagcgc accagggcgg cctcgatctg ctcgcgcgcg gccgggtcgc
     3601 cgcggtgcgc cagcacgtgg tcgcgcagcg cccaccgcgc ctccgaggcg acgccgccat
     3661 cccaggtggc gaagttggcc agcaaccggt ccagcgaggc ggagtcggct tgggtcctgg
     3721 cctcctgccc ggagagcgag gccgccgaga ggaaaagggc tgccgcggcg caggtgagga
     3781 tgaagcttcg aggtcggttc atggtcgtcc tcccttacgc cggaagctgg tagggcgcgc
     3841 ggtaggctcg cgagagcagc cgctcggccc ccggatcgtc ctggatggtc tcggttgccg
     3901 ggtcccaccg gatcgtgcgg ccggtctcga tggccaggac gcccaggtgc ccgacactcg
     3961 ccgaccggtg cgccacctcg gccggcgcga tcgtcagcgc ccggctgcgc acgcagtcga
     4021 ggaagttctg gaagtggtcg cggctgcggt agaggcgcgt ctcgttgggc ccgatgacct
     4081 cgttcacgag gtgcgccggc tcggcctcga agccgccccg gtccacccac acccaaccgc
     4141 ggtcgccgat ccacttcgcc ccgctctgga tctcggggta gccgccggcc aggacgaacg
     4201 gcgtcccgtc ggcgtacacg gcatccaccc agtagcgcct ggcagcgttg tagatgccgc
     4261 tggtcgggaa ctcgccggtg cccgagatct tcaccgggcc ggtgcgctcg aagtccatcc
     4321 cccagtgggc gatgtcgagg tggtgcccca c
//
LOCUS       DAOPQX010000329         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_21987_length_4345_cov_5.564103, whole genome shotgun sequence.
ACCESSION   DAOPQX010000329 DAOPQX010000000
VERSION     DAOPQX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21987_length_4345_cov_5.564103"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..783)
                     /locus_tag="PKK95_14360"
     CDS             complement(64..783)
                     /locus_tag="PKK95_14360"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HOC19452.1"
                     /translation="MTVAAAAPAIPRSALERELKFTLPASRAPFAARMLAALCRPDPR
                     FARAVVSTVYYDSPDLCLMAQKLNSDFLKTKVRLRWYSALSGEAGSASAFVEVKSRVG
                     CLRRKARAETALPGALLAAAPLTDPVFARALDVARTLEGDLPAWLLPVMMVRYERHRF
                     VEPVSGARVSLDFRIEAPRANRVLLGQGGPVRLGVAVVEVKGAGEELPRALRPLAHLG
                     ARRASFSKYGELGLALLRFRP"
     gene            complement(839..1540)
                     /locus_tag="PKK95_14365"
     CDS             complement(839..1540)
                     /locus_tag="PKK95_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOC19453.1"
                     /translation="MRTRWWIGNDVVDLAEPGAAGKERDRRFMDRVFTPSERAHILDS
                     AAPTIALWKTWAAKEAAYKIAAKLREGVVFAHRAFEVEPDRWPSRRARVRFEHLDIRV
                     RWVVARDYVHCIGHLASERAACAAFPPKAGGGAAEWWRLSAGILREGQLVDGQLTTAE
                     QASVHSTPSERARLLARRLVERWDVHGAEIVRLWRSWGWSPPVVARDGAPLTAFDVSL
                     SHDGRFVAAAVLGPA"
     gene            complement(1515..2264)
                     /locus_tag="PKK95_14370"
     CDS             complement(1515..2264)
                     /locus_tag="PKK95_14370"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC19454.1"
                     /translation="MLSTRERVGLRVQEAVAQGLILLSYYALAAWMRSRGYRIPRLRH
                     TRRAFGALMGPERGPWLICANHLTLIDSLVIQWAMAPGWRLFLRRDRFAWNLPDRHNI
                     SRTWWLRLLGYVGKCVPVLREGPPEEARRTLDKVAFLLSRGQAVVVFPEGGRSRIGRV
                     DPDRVMYGVGRMLQDVPAARVLCVYARGAGQAGYTDYPRPGEQFFIRAARVEPATELA
                     GMRGARDLARQIVAKLTAMEAEYFEDPLVDR"
     gene            complement(2269..2526)
                     /locus_tag="PKK95_14375"
     CDS             complement(2269..2526)
                     /locus_tag="PKK95_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOC19455.1"
                     /translation="MDSATAFGKVVEIIKPFVKNTAAFGEAGHGTRIIEDLGVNSARL
                     VDIILAFEDEFGIQVDDESADRVRTLGDAVQMILQKTAPAN"
     gene            complement(2548..3822)
                     /locus_tag="PKK95_14380"
     CDS             complement(2548..3822)
                     /locus_tag="PKK95_14380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOC19456.1"
                     /translation="MPERVVVTGMGVVAANAHGVDAFEQALREGRSGITFHPKLRDLG
                     FGCQVGGVPEVGDDLKRAYFPEDALRAMNSSMIYAGIAAIDCWRDAGFDLPAPGGGET
                     DWDTGAIVGTGIGGVDTVGEFVVPRVNEGKVRRLGSATVEQTMASSVSASIGGLLALG
                     GQVTTNSSACTTGTEAIVDAFHKIREGRAVRVIAGGTEGSSHYIWAGFDAMRVLARQS
                     NDRPAAASRPMSATAGGFVPSSGAGLLMLESLSSARARGARIHAEVLGGHVNCGGQRG
                     GGSMTAPNPEGARRCVIAALASAGVGPGEVDAINGHLTATMADTLEIGTWQRALGRRP
                     ESFPLINATKSLIGHGLGAAGGLEAVAVVLQLSRGFIHGSINCEDLHPDLAPYAGSIP
                     RATVAASPRVIAKASFGFGDVNGCLVFGKWDG"
     gene            complement(3815..4270)
                     /locus_tag="PKK95_14385"
     CDS             complement(3815..4270)
                     /locus_tag="PKK95_14385"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19457.1"
                     /translation="MQLTPAQILELLPQQRPFRFVDEILSADEERIVGRYTFRRDEPF
                     YQGHFPGRPITPGVILLESMCQVGVVSLGIYLFALDHSIEEAREWTTMFVDAESEFLK
                     SVLPGESVTIRGERVFWRRNKLRSRIEMFGADGTLVARTIASGVGVRNA"
BASE COUNT          632 a         1665 c         1471 g          577 t
ORIGIN      
        1 gcccccgtgt tcgcccccga gagaatggcg tacaagtcgg cgagttgttt ctgcataggt
       61 ttctcagggc ctgaagcgga gcagcgccag gcccagctca ccgtacttcg agaacgacgc
      121 gcgccgcgcc cccaggtggg ccagcggccg gagcgcgcgc ggcagctcct cgcccgcgcc
      181 cttcacctcg acgaccgcca cgccgaggcg gaccggcccg ccctgcccga gcagcacccg
      241 gttggcgcgg ggcgcctcga tccggaagtc gaggctgacg cgcgcgccgg acaccggctc
      301 gacgaagcgg tgccgctcgt accggaccat catcaccggc agcagccacg ccggcaggtc
      361 gccctccagc gtgcgcgcga cgtcgagcgc gcgggcgaac accgggtcgg tcagcggcgc
      421 cgccgccagc agggcgcccg gcagggccgt ctcggcccgg gccttgcgcc gcaggcaccc
      481 cacacgcgac ttcacctcga cgaaggccga ggcgctgccc gcctcgcccg acagcgcgct
      541 gtaccagcgg agccggacct tcgtcttgag gaagtcgctg ttcagcttct gcgccatcag
      601 gcacaggtcg ggcgagtcgt agtagacggt ggagaccacc gcccgcgcga accggggatc
      661 cgggcggcac agcgcggcca gcatgcgggc cgcgaagggc gcccgcgagg ccggcagcgt
      721 gaacttcaac tcgcgttcga gcgctgagcg ggggatggcg ggcgcagccg ccgcgacggt
      781 catcaaacgg gaaactatag catggacggt cggcgccgag gggggcgcgc cagggccgtc
      841 acgccggccc caacacggcg gcggccacga agcggccgtc gtgggagagg ctcacatcga
      901 atgcggtcag gggagcaccg tcgcgggcga ccacgggcgg gctccacccc cacgatcgcc
      961 acagccgcac gatctccgcg ccgtgcacgt cccaacgctc caccaggcgc cgggcgagca
     1021 ggcgcgcccg ctccgagggc gtggagtgaa cggacgcctg ctcggcggtg gtcagctggc
     1081 cgtccaccag ctgcccctcg cgaaggatgc ccgcagacag ccgccaccac tcggcggcgc
     1141 caccgccggc cttcggcggg aaggcggcgc acgctgcgcg ctcgctggcc aggtggccga
     1201 tgcagtggac gtagtcgcgg gccacgaccc accgcacccg gatgtcgagg tgctcgaagc
     1261 gcacgcgcgc ccggcggctc ggccaccggt cgggttcgac ctcgaaggcg cggtgggcga
     1321 acaccactcc ctcgcgcagc ttggcggcga tcttgtaggc tgcctccttc gccgcccacg
     1381 tcttccagag ggcgatggtc ggcgcggcgc tgtcgaggat gtgcgcccgc tcggagggcg
     1441 tgaagacgcg gtccatgaaa cggcggtcgc gttccttgcc ggcggcgccc ggctcggcca
     1501 ggtccacgac gtcgttaccg atccaccagc gggtcctcaa agtactcggc ctccatcgcg
     1561 gtcagcttcg ccacgatctg cctggccagg tcgcgggcgc cgcgcatccc cgccagctcc
     1621 gtcgcgggct ccacgcgcgc cgccctgatg aagaactgct cgcccggccg cgggtagtcg
     1681 gtgtagccgg cctgccccgc gcccctggcg tacacgcaca ggacccgcgc ggccgggacg
     1741 tcctggagca tgcggcccac gccgtacatc acccggtcgg ggtccacgcg gccgatccgg
     1801 cttcgcccgc cctcggggaa caccacgacc gcctgcccgc gcgacagcag gaacgccacc
     1861 ttgtcgagcg tccgccgcgc ctcctcgggc ggcccctcgc gcagcaccgg cacgcatttc
     1921 cccacgtatc ccagcagccg gagccaccag gtgcgcgaga tgttgtgccg gtcgggcagg
     1981 ttccaggcga agcggtcgcg gcgcaggaag aggcgccacc ccggcgccat cgcccactgg
     2041 atgaccagcg agtcgatgag cgtcaggtgg ttggcgcaga tcagccacgg cccccgctcg
     2101 ggccccatca gcgccccgaa cgcccgccgt gtgtgccgga ggcgcgggat gcggtagccc
     2161 cggctccgca tccacgccgc gagggcgtag taggagagga ggatcagccc ctgggccacc
     2221 gcctcctgca cccgcaggcc gacacgctcc ctcgtcgaca gcacggcctc agttggccgg
     2281 cgcggtcttc tgcaggatca tctggaccgc gtcgcccagc gtccgaacgc ggtcggcaga
     2341 ctcgtcgtcc acctggatcc cgaactcgtc ctcgaaggcc aggatgatgt ccaccaggcg
     2401 cgccgagttc acgccgaggt cctcgatgat ccgcgtgccg tgccccgcct cgccgaacgc
     2461 ggcggtgttc ttcacgaagg gcttgatgat ctcgacgacc ttcccgaatg ctgtcgcgct
     2521 gtccatcgct gtgtgtctcc tgctggttta cccgtcccac ttcccgaaga cgaggcaccc
     2581 gttgacgtcg ccgaagccga agctggcctt ggcgatcacg cgcggcgacg ccgccacggt
     2641 ggcgcggggg atgctacccg cgtaaggcgc gaggtccgga tgcaggtcct cgcagttgat
     2701 cgacccatga atgaagccgc ggctcagctg caacaccacc gcgaccgcct cgagcccccc
     2761 ggccgccccg agcccgtggc cgatgagcga cttggtggcg ttgatcagcg ggaacgattc
     2821 gggacgccgg ccgagcgccc gctgccacgt cccgatctcg agcgtgtcgg ccatggtggc
     2881 cgtgaggtgc ccgttgatcg cgtccacctc gccggggccg acgccggccg acgccagcgc
     2941 tgcgatcacg cagcgccgcg ctccctcggg gttgggcgcc gtcatgctgc cgccgccgcg
     3001 ctggccgccg cagttcacgt ggccgccgag cacctcggcg tggatccgcg ccccccgcgc
     3061 gcgcgccgac gacaggctct cgagcatcag cagcccggcc cccgacgagg ggacgaagcc
     3121 gccggcggtg gcgctcatcg gccgcgaggc cgcggcgggc cggtcgttcg actggcgcgc
     3181 gaggacgcgc atcgcgtcga atccggccca gatgtagtgc gacgagccct ccgtgccccc
     3241 cgcgatgacg cgaacggcgc gcccctcccg gatcttgtgg aaggcgtcca cgattgcctc
     3301 ggtgcccgtc gtgcaggcgc tgctgttggt ggtcacctgc cccccgagcg ccagcagccc
     3361 gccgatcgac gcgctcaccg agctggccat cgtctgctcg acggtggcgc tgcccaggcg
     3421 ccgcaccttg ccctcgttga cccgcggcac cacgaactcg ccgacggtgt ccacgccgcc
     3481 gatgcccgtg ccgacgatcg cgcccgtgtc ccagtccgtc tcgccgccgc ccggcgccgg
     3541 gaggtcgaac cccgcgtcgc gccagcagtc gatcgcggcg atgccggcgt agatcatgct
     3601 gctgttcatc gcgcgcagcg cgtcctccgg gaagtacgcg cgcttcaggt cgtcgccgac
     3661 ctccggcacg ccgcccacct ggcagccgaa ccccaggtcg cgcagcttcg ggtggaaggt
     3721 gatcccggac cgtccctcgc gcagcgcctg ctcgaacgcg tccacgccgt gcgcgttggc
     3781 cgcgacgacc cccattcccg tcaccacgac tcgttcaggc attcctgact cccaccccgg
     3841 aagcgatcgt ccgggcgacg agggtgccgt cggccccgaa catctcgatg cgcgaccgga
     3901 gcttgttccg ccgccagaac acccgttcgc cgcggatcgt cacgctctcg cccggcagca
     3961 ccgacttgag gaactccgac tcggcatcga cgaacatcgt cgtccactcc cgcgcctcct
     4021 cgatggagtg atcgagcgcg aagaggtaga tgccgagcga cacgaccccc acctggcaca
     4081 tcgactccag caggatcaca ccgggcgtaa tcggccgccc cgggaagtgc ccctggtaga
     4141 acggctcgtc gcgccggaag gtgtaccgcc caacgatccg ctcctcgtcc gccgaaagga
     4201 tctcgtccac gaagcggaac ggccgctgct gcggcagcaa ctcgagaatc tgggccgggg
     4261 tcagctgcat atcgcgctcg cttcctaccg cttcatctgc ccgccgaccg agatgccagt
     4321 caggaccagg tggaacgtcg gctgg
//
LOCUS       DAOPQX010000330         4329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22087_length_4329_cov_3.618624, whole genome shotgun sequence.
ACCESSION   DAOPQX010000330 DAOPQX010000000
VERSION     DAOPQX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22087_length_4329_cov_3.618624"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..892)
                     /locus_tag="PKK95_14390"
     CDS             complement(176..892)
                     /locus_tag="PKK95_14390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC19458.1"
                     /translation="MTEGPVFYRSFAGKRLTGAGARRRSRIEAHRLGILHQHRTPPGD
                     FLEVGPGQGTLARAAVAAGWRYVGIEPSPALAPGLARQGLTIVQAWTPPFPVRDACCD
                     VLWADQVLEHMNGATEARAFASEIVRVLRPGGVAIVVVPDYTKERWFFWDIDYTHNFV
                     TTERRVRQLLEDAGLTVSAMNRAIGAATGPLRAVLAAAALPANTPGLDALMRAVGKDE
                     LLFQVRKNLFQTLLFVGRRP"
     gene            complement(894..1883)
                     /locus_tag="PKK95_14395"
     CDS             complement(894..1883)
                     /locus_tag="PKK95_14395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOC19459.1"
                     /translation="MTPELSVVIPIKNEEENLPRLYEELVGALEGFRPFELVFVEDGS
                     TDGSFPVLASLQSSDPRVRVIRFRRNFGQTAAFAAGFAFARGRYIVTSDGDLQNDPSD
                     IPRMVERLEQGFDIVAGWRKDRKDPFLSRRLPSMIANKLISMATGVALHDYGCSLKVF
                     RAEVVKPMKLYGEMHRFLPAIASEMGVRIDEVVVNHRARLHGHSNYGISRTVRVVLDL
                     LTVKFLLSYSTRPLQIFGLVGGSMGALGLAITGWLAVQRLFLYRSIGDRPLLLLGILL
                     IFTGVQLVTLGLLAEMQARTYHESQDKPIYVIREVRETLSAEPASPQLAGSRR"
     gene            complement(1899..2504)
                     /locus_tag="PKK95_14400"
     CDS             complement(1899..2504)
                     /locus_tag="PKK95_14400"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOC19460.1"
                     /translation="MKGLAFWAQSTVAAWGGLGLLFVAFLDCSVLSLPEAVDLLLVWL
                     VIRNESAAAYYVGAATLGSLAGTMVLFWVTRKGGEAFLRKRFAARRVERGMALFRRYG
                     MGIVAVSAVLPPPTPVKLFVLLAGVSGAGATRFALAVAAGRLLRFTIIGALALWFGDE
                     AIGYIREHALTLGLLALAAVLGASLAYLAWRRWRRQDAGAI"
     gene            2582..4123
                     /locus_tag="PKK95_14405"
     CDS             2582..4123
                     /locus_tag="PKK95_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19461.1"
                     /translation="MTERARGLAATGPEIIADGPADSTWNRRAAATVAALALVVGSVA
                     VWVYANAGLTLSHYDAKAHLVVARRVLDSMTPGWKQIGAVWLPLPHLLNLLPVQVDVF
                     YRTGASAVAMSVLSFALAAWACARLVLHATGSRAGAVAAGAIVVFNPNMLYLQATPMT
                     EALLLGLTSLSVLLVVRWVDNPSAARRRAAGAAIVLACLTRYEAWPVTAAALGLAGIA
                     FLRRGLRPGEAARHVAALTVYPVLTVLAFLVQSKLTIGKWFVTGGFFVADNPDLGRPF
                     RALISIAWGARALDGWVVIAAGSMGAAALIATALRRKERASALVLLALFATAALPWYA
                     FFQGHPFRIRYMVPLLPALAVAAGSLVGMATRFQWPAAAIVVALAVLQVRPFDPRAPM
                     VIEAQWDRPNTFERQKVTAYLAHHRRGEKVLVSFGSLSHYVQELSHAGFAVRDFIHEG
                     NNVIWNASLEHPSAHVGWILIEERAEGGDQLAARAASTPSFLERFERVAEGGGVALYR
                     RTGAP"
BASE COUNT          653 a         1551 c         1501 g          624 t
ORIGIN      
        1 gctgaacggg gaactgcgca cctacgagag ccgaccctgg ctgcggccgc cgtgagcgac
       61 acgatcttcg gcggatcgtt ctggacgggt ggggtggggt tggtgggccc gccgcacgac
      121 agcgcggcca ctgccgccgc agcgcagcac gcgccgagcg ccacccatcg cgcgttcacg
      181 ggcgccgccc cacgaacagc agggtctgga agaggttctt cctcacctgg aacagcagct
      241 cgtccttccc taccgcccgc atcagggcgt cgaggcccgg cgtgttcgcg ggaagcgccg
      301 cggcggcgag caccgcccgc agcggcccgg tcgccgcgcc gatggcccgg ttcatcgcgc
      361 tcaccgtcag ccccgcgtcc tcgagcagct gccgcacgcg gcgttcggtc gtcacgaagt
      421 tgtgcgtgta gtcgatatcc cagaagaacc agcgttcctt ggtgtagtcg ggaacgacga
      481 cgatcgcgac gccgccgggg cgcagcaccc ggacgatctc cgacgcgaac gcccgggcct
      541 cggtcgcccc gttcatgtgc tcgagcacct ggtcggccca gagcacgtcg cagcaggcgt
      601 cgcggacggg gaacggcggc gtccaggcct ggacgatcgt cagcccctgg cgagccagtc
      661 cgggcgccag cgcggggctc ggctcgattc ccacgtagcg ccacccggcc gccacggccg
      721 cgcgggccag cgtcccctgg ccgggaccga cctcgaggaa gtcgcccggg ggcgtgcggt
      781 gctggtgcag gatcccgagg cgatgggcct cgatgcgcga acggcggcgt gcgccggcgc
      841 ccgtgaggcg cttcccggcg aacgaccggt agaagaccgg accttctgtc atgtcaccgg
      901 cgcgagccgg ccagctgcgg ggacgccggc tcggcggaga gcgtctcccg gacctcccgg
      961 atgacgtaga tcggcttgtc ctgcgactcg tggtaggtgc gcgcctgcat ctcggcgagc
     1021 aggccgagcg tgaccagctg cacgccggtg aagatcaaga ggattccgag aagcagcagc
     1081 ggccgatccc cgatcgagcg gtagaggaac agccgctgca cggcaagcca cccggtgatc
     1141 gccaggccga gcgcccccat cgatccgccg acgaggccga agatctgaag cggccgcgtc
     1201 gagtagctga gcaggaactt cacggtcagc aggtcgagca ccaccctgac ggttctcgag
     1261 atcccgtagt tggagtggcc gtgcagccgc gcccggtggt tcaccaccac ctcgtcgatc
     1321 cgcaccccca tctcgctcgc aatcgcgggc aggaagcggt gcatctcgcc gtacagcttc
     1381 atcggcttca cgacctcggc ccggaacacc ttcagggagc agccgtagtc gtgcagggcg
     1441 acgccggtcg ccatcgagat cagcttgttg gcgatcatcg acggaagccg ccgcgacagg
     1501 aacgggtcct tccggtcctt gcgccagccc gccacgatgt cgaacccctg ctcgaggcgc
     1561 tcgaccatgc gcgggatgtc ggacggatcg ttctgcagat cgccgtcgct cgtcacgatg
     1621 taacggcccc gggcgaacgc gaacccggcg gcgaaggcgg ccgtctgccc gaagttgcgg
     1681 cggaagcgga tgacccggac gcgggggtcc gaactctgta gcgacgccag caccgggaag
     1741 ctgccgtcag tgctgccgtc ctcgacgaag accagctcga acgggcggaa gccctcgagg
     1801 gctcccacca gctcctcgta gagccgcggt aggttctcct cttcgttctt gatcgggatg
     1861 acgacggaca gttcgggcgt catgcgaaaa cgctcggatt atatcgcacc ggcatcctgg
     1921 cgccgccagc ggcgccaggc gaggtacgcg agcgaggcgc ccagcacggc cgcaagcgcc
     1981 agcagcccga gcgtcagcgc gtgctccctg atgtagccga tcgcctcgtc accgaaccac
     2041 agggcaagcg cgccgatgat cgtgaagcgc aggagccgtc cggccgcgac ggccagcgcg
     2101 aaacgggtcg cgccggcccc cgacaccccg gccagcagca cgaagagctt gaccggcgtg
     2161 ggcggcggca gcaccgccga aacggcgacg atgcccatcc cgtagcgccg gaacagcgcc
     2221 atcccccgct ccacccggcg cgccgcgaag cgcttccgga ggaaggcctc gccccccttc
     2281 cgcgtgaccc agaacaggac catcgtgccg gccagtgatc cgagcgtcgc ggcgcccacg
     2341 taataggcgg ccgctgactc gttgcggatg accagccaga ccagcagcag gtcgaccgcc
     2401 tcgggcagcg acaggaccga gcagtcgaga aaggcgacga acagcagccc caacccgccc
     2461 caggcggcca cggtgctctg ggcccagaac gcgagcccct tcatgccctt cgcccccgcc
     2521 gccccggacg gtcccgcaac cgaagtacgc tagagttgag gaccatgtgc ggtggatccc
     2581 agtgacggaa cgcgctcgcg gccttgccgc gacaggaccc gagattatag ccgacggccc
     2641 cgccgactcc acctggaacc gccgcgcggc cgccacggtt gcggctctgg cgctggtcgt
     2701 gggatcggtg gcggtctggg tctacgcgaa cgccggcctc accctcagcc actacgacgc
     2761 gaaggcgcac ctggtggtcg ccaggcgggt gctcgacagc atgacgcctg gctggaaaca
     2821 gatcggcgcg gtctggctgc ccctgcccca cctgctcaac ctgctgcccg tgcaggtgga
     2881 cgtgttctat cggaccggcg cctcggcggt ggccatgtcg gtgctctcct tcgcgctcgc
     2941 ggcgtgggcg tgcgctcgcc tggtgctgca tgcaaccggg tcgagggccg gcgcggtggc
     3001 tgccggcgca atcgtcgtct tcaacccgaa catgctctac ctgcaggcca cgccgatgac
     3061 cgaggccctt ctgctcgggc tgacgtcgct ctcggtgctg ctggtggtgc gttgggtgga
     3121 caatccgtcg gccgcccgcc ggcgcgcggc cggcgccgcc atcgtcctcg cctgcctgac
     3181 ccgctacgag gcgtggccgg tgactgccgc cgcccttggc ctggcgggca tcgcgttcct
     3241 gcggcgaggc ctgcggccag gcgaggcagc gcgccacgtt gccgccctca ccgtctaccc
     3301 cgtgctcacc gtgctggcct tcctggtaca gagcaagctc accatcggga agtggttcgt
     3361 gacgggtggg ttcttcgtcg ccgacaaccc cgacctcgga cggcccttca gggcgctgat
     3421 ctcaatcgcg tggggtgcgc gggcactcga cgggtgggtg gtgatcgcgg ccggaagcat
     3481 gggagcggca gccctgatcg cgacggcgct gcgccggaag gaacgggcgt cggcgctggt
     3541 gctgctcgcc ctgttcgcaa cggccgcgct gccgtggtac gccttcttcc aggggcaccc
     3601 gttccgcatc cggtacatgg tgccgctgct gccggcgctg gccgtcgccg ccgggtcgct
     3661 cgtggggatg gcgacccgct tccagtggcc cgcggccgcg atcgtggtgg cgcttgccgt
     3721 cctgcaggtg cggccattcg acccccgcgc gccgatggtg atcgaagcgc agtgggaccg
     3781 gcccaacacg ttcgagcgcc agaaggtgac ggcgtacctc gcccaccacc gccgcggcga
     3841 gaaggtgctc gtcagcttcg ggtcgctgtc acactacgtg caggagctgt cgcacgctgg
     3901 ctttgccgtg cgcgacttca tccacgaggg gaacaacgtg atctggaacg cctccctcga
     3961 gcacccctcc gcccacgttg gatggatcct gatcgaggag cgggctgagg gtggcgacca
     4021 attggccgcg cgggcggcca gcacgccgtc gttcctggaa cgcttcgagc gggtggccga
     4081 ggggggcggg gtcgcgctgt acagaaggac cggcgcgccg taggggaaca gcgcgccggt
     4141 ctcccttctc ccgttccggc cgcgacgcgg cccgtcagaa cttgatctgc agcaccccct
     4201 gccacgtgat gccgcccagg tcgatccggt ccccgttgaa cagcgatgcg tccagttctc
     4261 cttcggcgcg ctggtagcgc acctcgccgc cgatcgagaa gttgtgcgtc agcggcagcc
     4321 ggatgccgc
//
LOCUS       DAOPQX010000331         4313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22199_length_4313_cov_3.267262, whole genome shotgun sequence.
ACCESSION   DAOPQX010000331 DAOPQX010000000
VERSION     DAOPQX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22199_length_4313_cov_3.267262"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKK95_14410"
     CDS             complement(<1..345)
                     /locus_tag="PKK95_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19462.1"
                     /translation="MPGTPLAVRALLRAPFVTMVAIVSLALGIGANTAIFSLFNEILL
                     RPLPVHDPARLVNLGAPGPKPGSQSCNQAGSCAQVFSYPMFKDLQRVQTVFTGIAAHR
                     LFGANLSFKGQTL"
     gene            333..608
                     /locus_tag="PKK95_14415"
     CDS             333..608
                     /locus_tag="PKK95_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19463.1"
                     /translation="MCRAYELGRQPAGIGSRLAHGAARKDDNRSLRRRWFLEFLNLSL
                     IFAAGWLVYRRPQRERLAFGLLVASVVLTVVLFSFATRTSLLPGMNY"
     gene            662..2152
                     /locus_tag="PKK95_14420"
     CDS             662..2152
                     /locus_tag="PKK95_14420"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HOC19464.1"
                     /translation="MQRQWIYTVLAVAVLALAVVPIGTAVFVLGFVYGDSPCVMCWEQ
                     RIAMALIALIGLFVLRYGPRPRYVGLSILVAGYGAFMSLRHTAMHASRDIGQGFSLEI
                     LGAHTYTWALFVFWAAILMMGALLMAVREGDAGGVIRKLRPLEKLAAVVFLVVIAGNL
                     VQAFASTGPPPFMGQGDPVRFSFNPAHWVWSLEEYSAAPVGLRGRWAASKPDASKADP
                     DPSLGPFVWASPLKLRAERTLGPGFNGTPTGLDYEEASDRFLLTTEDGVYLADGALAR
                     PLRHTIVDVGYSADLARFAGAAFLEAGTVMAVSDNKSFVILRESDKADASKNFRYFRE
                     SFDRFDEVRRGRFGTVRARMMYVKSAAYDPARQSIYTVTVPNERNRGLVISRFDRRDL
                     TLSEEFVARLSPEADVRLLGERTLDELFVTAAAVRGGRLFALSAAYSTLLEIDLDARA
                     VVGARTVPGLSRPAGIAFKGDELWVVTEEGKVLTFDLTQAPRPASS"
     gene            complement(2121..2945)
                     /locus_tag="PKK95_14425"
     CDS             complement(2121..2945)
                     /locus_tag="PKK95_14425"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOC19465.1"
                     /translation="MDWFYGLGPIQQALLATVGTWLVTALGASLVLITTNVSQRYMDG
                     SLGMAAGVMIAASFWSLLAPSIELAEEAGYGGLKWVPPLVGFVAGALFLRLVDRILPH
                     LHPGLAEAEGPHTKWRRTTLLVLAITLHNIPEGLAVGVAFGAAANADLSEAQRLSQIG
                     AAVALAIGIGLQNFPEGTAVALPLRREGVGRFKSLWWGQLSAIVEPVAGVAGAALVLL
                     MMPILPYALAFAAGAMIYVVTEELIPESQSNGHVDLATMGVIAGFSIMMMLDVGLG"
     gene            complement(2999..3328)
                     /locus_tag="PKK95_14430"
     CDS             complement(2999..3328)
                     /locus_tag="PKK95_14430"
                     /inference="COORDINATES: protein motif:HMM:NF039419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HOC19466.1"
                     /translation="MKSLTLLGIALALVVGLAASPAAVEETTLSGKVVCAKCTLKKAD
                     AKECQDVLVVKGDGDAAVEYYIVKNEVAKEFGHTCTGEKPATVTGTVSEKDGKKWIAA
                     TKIEEIK"
     gene            3502..>4313
                     /locus_tag="PKK95_14435"
     CDS             3502..>4313
                     /locus_tag="PKK95_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19467.1"
                     /translation="MRCDPLEVTLDVVPRARFDMLDLRSCLPAEKRQRLNAYPRSLYW
                     SSHTTAGFLEPATAARLFVGPGVAAYLQAFRALFPEGADYEHDHLERRLELPPDKRLV
                     EPRNADSHLAFIAAGLRPCVISRNGPGPGVCFVDLDGINGGRPRTRRTRIIGFTEEEE
                     IARARIEVPVSTHAVDSVNLKDPRLGVYDQLREFVRAHDVSKGRLRLALAEHERHAGL
                     TVNEYETLLMRHDLPEVLRNPLRFAIERGRHALAEPRKVPAKTLGYAKYDLVR"
BASE COUNT          633 a         1484 c         1514 g          682 t
ORIGIN      
        1 aagcgtctgg cccttgaagc tgaggttggc cccgaacaac cggtgggcgg cgattccggt
       61 gaagaccgtc tggacccgct ggaggtcctt gaacatcggg tagctgaaca cctgggcgca
      121 cgaaccggcc tggttgcacg actgggagcc gggcttgggg ccgggtgcgc caaggttcac
      181 cagccgtgcc gggtcgtgga ccggcaacgg gcgcaggagg atttcgttga agagcgagaa
      241 gatcgcggta ttggccccga tgccgagcgc cagagagacg atcgcgacca tggtgacgaa
      301 gggggcgcgg agcagcgccc tgacggcgag gggtgtgccg ggcatatgag cttggacggc
      361 agccggcggg gataggttcg cgacttgcgc acggcgccgc gaggaaggat gataaccgct
      421 cgctacggag gaggtggttt ctggagttcc tcaacctgtc gctgatcttc gccgccggct
      481 ggctggtcta caggcgcccg cagcgcgagc gcctcgcctt cgggctgctc gtggcgagcg
      541 tcgtcctcac ggtggtgctg ttctcgttcg ccacgcgcac gtccctgctg ccggggatga
      601 attactagga ttcgggattc gggattcggg attcagggat cagggatcag ggaaggatca
      661 catgcagcga cagtggatct acaccgtgct ggccgtcgcg gtgctcgcgc tggcggtcgt
      721 gccaatcggg acggcggtgt tcgtcctcgg gttcgtgtac ggtgactcgc cgtgcgtgat
      781 gtgctgggag cagcggatcg cgatggcgct gatcgcactc atcgggctgt tcgtgctgcg
      841 ctacgggccg cgcccgcggt acgtcgggct ctcgatcctg gtcgccggct acggggcgtt
      901 catgtcgctc cgccacacgg ccatgcacgc ctcgcgcgac attggccagg ggttcagcct
      961 cgagattctc ggcgcccaca cctacacctg ggcgctgttc gtcttctggg cggccatcct
     1021 gatgatgggg gcgctgctga tggcggtgcg cgagggcgac gccgggggcg tcatccgcaa
     1081 gctgcgcccg ctcgagaagc tggcggcggt tgtgttcctg gtggtgatcg ccggcaacct
     1141 cgtgcaggca ttcgcgagca ccgggccgcc gcccttcatg ggccagggcg atcccgtgcg
     1201 gttctccttc aacccggccc actgggtgtg gtcgctcgag gagtactcgg cggcccccgt
     1261 cggcctgcgc ggccgctggg ccgcctccaa gcccgacgcg tcgaaggccg acccggatcc
     1321 ctctctcggc cccttcgtgt gggcgtcgcc cctgaagctg cgcgccgagc gcactctcgg
     1381 gcccgggttc aacgggacgc caacgggact cgactacgag gaggcctcgg accgcttcct
     1441 gctgaccacc gaggacggcg tgtacctggc cgacggcgcc ctcgcccgcc cgctgcggca
     1501 caccatcgta gacgtcggct actcggcgga cctggcgcgc ttcgcggggg cggcgttcct
     1561 ggaggcgggg acggtgatgg cggtcagcga caacaagagc ttcgtgatcc tgcgcgagag
     1621 cgacaaggcc gacgcgtcga agaacttccg atacttccgc gagtcgttcg atcggttcga
     1681 cgaggttcgg cgaggccgct tcggcaccgt ccgcgcccgg atgatgtacg tcaagtcggc
     1741 ggcgtacgac ccggcgagac aatcgatcta caccgtcacg gtgccgaacg agcgcaaccg
     1801 tggactggtg atctcgcgat tcgaccgccg cgacctgacg ctgtccgagg agttcgtcgc
     1861 ccggctgtcc cccgaggccg acgtgcggct gctcggcgag cggacgctcg acgagctgtt
     1921 cgtcaccgct gccgcggtgc gcggcggccg gctcttcgcg ctcagcgccg cctactcgac
     1981 cctcctcgag atcgacctgg acgcgcgggc cgtcgtgggc gcccgcaccg tgccgggact
     2041 gtcgcgtccg gcaggcatcg ccttcaaggg cgacgagctg tgggtggtga cggaggaggg
     2101 gaaggtgctg accttcgacc tcacccaagc cccacgtcca gcatcatcat gatcgagaag
     2161 ccggcgatga cccccatcgt cgccaggtcg acgtggccgt tcgactgcga ctcggggatc
     2221 agctcctcgg tgacgacata gatcatggcg ccggcggcga acgcgagcgc gtagggcagg
     2281 atcggcatca tcagcaggac gagggccgcg ccggccaccc ccgcgaccgg ctcgacgatc
     2341 gccgacagct gcccccacca cagcgacttg aaccgcccga cgccctcgcg acggagcggc
     2401 agggcgaccg cggtcccctc gggaaaattc tgcaggccaa tcccgatcgc cagcgccacg
     2461 gcggccccga tctgcgaaag gcgctgcgcc tcggagaggt cggcgttggc cgcggcgccg
     2521 aacgccaccc cgacggccag tccctcggga atgttgtgaa gcgtgatggc gaggaccagc
     2581 agcgtcgtcc gccgccactt ggtgtgcggc ccctcggcct cggccaggcc gggatggagg
     2641 tgcggcagga tccggtcgac cagccggagg aagagcgcgc ccgccacgaa gccgacgagc
     2701 ggcggcaccc acttcaggcc gccgtagccg gcctcctcgg ccaactcgat ggagggcgcg
     2761 agcaggctcc agaagctggc ggcgatcatc acgccggccg ccatgcccag cgagccgtcc
     2821 atgtagcgct gcgagacgtt ggtggtgatc agcaccagtg acgcgccgag cgcggtcacg
     2881 agccaggtgc cgaccgtcgc gagaagcgcc tgctggatcg gaccgaggcc gtagaaccag
     2941 tccaccctcg tcctccgggg aaaaggggcg ccgcgggcgc ccccggccgc ctgaccgact
     3001 acttgatctc ctcgatcttc gtcgcggcaa tccacttctt gccgtctttc tcggacacgg
     3061 tgccggtcac cgtggcgggc ttctcacccg tgcaggtgtg accgaactcc ttggccacct
     3121 cgttcttgac gatgtagtac tccacggccg cgtcgccgtc gcccttgacg acgagcacgt
     3181 cctggcactc cttcgcgtcg gccttcttca gcgtgcactt cgcgcacacg accttgccgc
     3241 tcaatgtcgt ctcctcgacg gcggcgggcg atgcggccag tccgacgacg agggcgagcg
     3301 cgatcccgag cagggtcaag ctcttcatcg gtcctcctcc gagaacggcg ggcgcggccc
     3361 ggaaggggcc tcccgcgggt tgtctccgga tacgggctag cgtagccgga acggccgggc
     3421 tgcgcaatcc cggccgctcc tggtgtatcc ttgacgatgg cgcttctccg gggcttcggg
     3481 ccggagaggc tcgaggccct gatgcgttgc gatcccctgg aagtcacgct ggacgtcgtt
     3541 ccgcgcgcgc ggttcgacat gctcgatctt cggagctgcc tgcctgctga gaagcgccag
     3601 cgcctcaacg cctacccgcg cagcctctac tggtcgtccc acacgacggc gggtttcctc
     3661 gagccggcca cggccgcgcg tctgttcgtc ggaccgggcg tggcggccta cctgcaggcc
     3721 ttccgggcgc tgttccccga gggagccgac tacgagcacg atcacctcga gcggcgcctc
     3781 gagcttcccc ccgacaagcg gctggtcgag ccgcgcaacg ccgactcgca cctcgccttc
     3841 atcgccgcgg ggctgcgccc gtgcgtcatc tcgcgcaacg ggccggggcc cggcgtctgc
     3901 ttcgtcgacc tggacgggat caacggcggg cggccacgca cccggcggac ccggatcatc
     3961 gggttcaccg aggaggagga gatcgccagg gcccgcatcg aggtgccagt ctcgacccac
     4021 gccgtggact cggtcaacct gaaggacccg cggctgggcg tctacgatca gctgcgggag
     4081 ttcgtccgcg cgcacgacgt gtcgaagggc cgcctgcgcc ttgcgctcgc cgagcacgag
     4141 cggcacgccg ggctgaccgt gaacgagtac gagacgctgc tgatgcgcca cgacctgccc
     4201 gaggtactgc gcaacccgct gcggttcgcc atcgagcgcg ggcgccacgc gctggccgag
     4261 ccgcggaagg tgccggccaa gaccctcggt tacgcgaagt acgacctggt gcg
//
LOCUS       DAOPQX010000332         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22221_length_4310_cov_3.988014, whole genome shotgun sequence.
ACCESSION   DAOPQX010000332 DAOPQX010000000
VERSION     DAOPQX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22221_length_4310_cov_3.988014"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1431
                     /locus_tag="PKK95_14440"
     CDS             226..1431
                     /locus_tag="PKK95_14440"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC19468.1"
                     /translation="MLHRVGAALAFPDFRKLWFGAATSSIGTWMQSVAQNWLVLTISG
                     SAFYLGLDAFLGQLPILLFTLLGGVVADRHDRRRVLLGSQYVQMSTAFALALLVALGA
                     VQVWQILALSFVTGCAQAFGGPAHQSLMPSLVDRDRLPNAIALNSIQFNLARVIGPLL
                     AGLALASFGMVFCFTANGLSFLAVIAALWTLHVPAREQAAPLPLAQELRTGLTYVRRE
                     GPLFGLTLLAFAVTFFGTPLLTLLPVFAQSVFRQGVEGYSRMMVFSGAGAVTGAITVA
                     WLGRFPRMGTWALLTQAGFGVLIVAFALVRVPALSYLLLFAAGAALIVVFSLMNSLVQ
                     LLAPNPMRGRVMSIYMVAFRGGMPLGSLASGALANLVSAPAVLALNGSLLVGVAALFL
                     LTYEKLRTV"
     assembly_gap    1581..1680
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2040..4106
                     /locus_tag="PKK95_14445"
     CDS             2040..4106
                     /locus_tag="PKK95_14445"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOC19469.1"
                     /translation="MLLRRGVLTAVALAVALTAVVRSDEGMWTFDKAPLARINAALGT
                     AIDQAWLDRIQAGAVRLGGCSASLVSSEGLVLTNQHCVRSCLQSFSTPGRDRVAEGFF
                     TKGRDDERTCPGQTAEVLLSIEDVTARVMAATAGTTGREFVRARSAEQAAIEKAGCGA
                     DASLRCQVVTLHRGGQYKLYKYRRYSDVRLVYVPEHQAVVFGGDPDNFNFPRFALDAA
                     FLRVYENGKPASTPSFLRWTPRAPKPGEPVFVAGNPGSTSRLETVAQLETARDVQLPA
                     LIKRYSELRGRLLRFADESAEHKRMTRDAIRGVENNVKRLWGQHQTLADPAFMDAKRA
                     AERELIARSAGKAGSEPVAGAVAAIAAVQPQRRALMPFYDQLEQFAGASSVLYNYART
                     LVRAALARSAAAPGDRLPEYSDARLAVLGKSLTEPRAIEPELETLYLGFWLSKAREVM
                     GTDAPETKTLLGRESPEGLAGRLVAGTKLADPAVRKALWDGGEAAVRASDDPLIRFVL
                     ATDAQARAVRAAWEERAVAPTDRAAERLALARFEAYGDDVYPDATFTLRLSYGRIEGW
                     KEGDRVVEPVTRIAGLWERATGAEPYVVSPGLAAARDRLAPGTVFTVATTNDIVGGNS
                     GSPLLDAQGNVIGAIFDGNLHSNGGTYGYDPQLNRAVAVSAAVVQEALLKVYDQPHLV
                     KELSGK"
BASE COUNT          589 a         1485 c         1433 g          703 t
ORIGIN      
        1 tccgtccgca gcggcgatgg tgcgatcgag gcggcggaca cggagcaggg cttcaaggtc
       61 ggtgcgagcg agagcagcct cagccatttc gcttttcctt cgcctattag cgaaagaata
      121 gcgtaagtgg tgcgggcggt caagaaaaag gctcagggct caaggctcag ggctcagggt
      181 tcgccatggg gaagccaggc gagcgcaggc atagaatggg cgctcatgct gcaccgcgtc
      241 ggcgccgccc tcgccttccc cgacttccgc aagctctggt tcggcgcggc cacctcgagc
      301 attggaacct ggatgcagag cgtcgcgcag aactggctgg tgctgacgat cagcggatct
      361 gcgttctacc tggggctcga cgccttcctc gggcagctcc cgatcctgtt gttcaccctg
      421 ctcggggggg tggttgccga ccgccacgac cgccgccgcg tgctcctcgg ctcgcagtac
      481 gtccagatga gcaccgcctt cgcgctcgcg ctgctcgtcg ccctcggcgc cgtgcaggtg
      541 tggcaaatcc tcgccctctc cttcgtcacc gggtgcgcgc aggcgttcgg cgggccggcc
      601 caccagtcgc tgatgccgtc gctcgtcgac cgcgaccgcc tgcccaacgc gattgccctc
      661 aactcgatcc agttcaacct cgcccgcgtg atcggaccgc tgctggctgg cctcgcgctc
      721 gcctcgttcg gcatggtctt ctgcttcacg gccaacggcc tgtcgttcct cgcggtcatc
      781 gccgcgctct ggacgcttca cgtccccgcc cgcgaacagg ccgctcccct ccccctcgcc
      841 caggaactgc ggaccggcct cacctacgtg cggcgcgagg ggcctctctt cggcctgacg
      901 ctgctcgcct tcgccgtgac ctttttcggc acgccgctgc tgacgctgct gccggtcttc
      961 gcgcagagcg tgttccgcca gggcgtggag ggatacagcc ggatgatggt gttctcgggt
     1021 gccggggccg tgaccggcgc gattaccgtg gcgtggctcg ggcggtttcc tcgcatgggg
     1081 acctgggcgc tcctgacgca ggcggggttc ggcgtgttga tcgtcgcctt cgcgctcgtc
     1141 cgcgtccccg ccctcagcta cctgctgctg ttcgccgccg gcgccgccct catcgtcgtc
     1201 ttctcgctga tgaactcgct cgtgcagctg ctggccccca acccgatgcg cggacgcgtg
     1261 atgagcatct acatggtggc cttccgcgga gggatgccgc tcggcagcct cgcgagcggt
     1321 gcgctggcaa acctcgtctc ggccccggcc gtgcttgcgc tgaacggctc gctgctggtg
     1381 ggggtggctg ccctgtttct gctcacgtac gagaaactga ggacggtgtg atgagcagca
     1441 gacagaggga tcagggatca gatcgtgccg gaggtgcaga cggtgcagga ggtgccgaag
     1501 gtgcggtgca ggaggtgccg aaggggcggt gcaggaggtg ccgaaggggc ggtgcaggag
     1561 gtgccgaagg ggcggtgcag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 gaggtgccga aggggcggtg ctgatggtgc cggaggggcg gtgctgatgg tgccggaggg
     1741 gcggtgctga tggtgcccgg aggggcggtc ctgatggtgt tggaggtgcg gcgtcacgtc
     1801 tgtcatgtac gtttcaatac agaggcgcag cgatttctgg aatgatccga gatctctgcg
     1861 tacgcctccg cgtctccgcg cctctgcggt taacgtggac aagccgttac ctctgtcacg
     1921 tacgtgtcaa cgcagggatc tccagaacca ttcgagatct cggcgtacgt ctctgtgtct
     1981 ctgtgcctct gtggttgacg tgatccgata ggcaagggtc gcccccaacg agaggaacca
     2041 tgctgttacg ccgcggagta ctgaccgccg tcgctctcgc cgttgccctc accgccgtcg
     2101 ttcgcagcga cgagggcatg tggaccttcg acaaggcgcc gctcgcccgc atcaacgctg
     2161 cgctcgggac ggcgatcgac caggcgtggc tcgaccgcat ccaggcgggc gccgtgcgcc
     2221 tcggcggctg ctcggcgtcg ctcgtctcgt ccgaggggct cgtcctcacc aaccagcact
     2281 gcgtccgctc gtgcctgcag tcgttctcca ctcccggccg cgaccgggtg gccgaggggt
     2341 tcttcaccaa ggggcgcgat gacgagcgga cctgccccgg ccagaccgcg gaggtcctct
     2401 tgtcgatcga ggacgtcacc gcgcgcgtga tggccgccac cgccgggaca acggggcgcg
     2461 agttcgtgcg cgcgcgaagc gccgagcagg cggcgatcga gaaggccggc tgcggcgccg
     2521 acgcgtcgct ccgctgccag gtggtgacgc tccaccgcgg gggccaatac aagctctaca
     2581 agtaccggcg ctattcggac gtccgcctcg tgtacgtgcc cgagcaccag gcggtcgtct
     2641 tcggcggcga ccccgacaat ttcaacttcc cgcgcttcgc gctcgacgcc gccttcctgc
     2701 gcgtctacga gaacggcaag cccgcctcga cgccgtcgtt cctgcgctgg acgccgcgcg
     2761 cgccgaagcc gggcgagccc gtcttcgtcg ccgggaatcc cggcagcacc tcccgtctcg
     2821 aaacggtggc gcagctcgag accgcgcgcg acgtgcagct gcccgccctc atcaagcgat
     2881 actccgagct gcggggccgc ctgcttcgct tcgccgacga gagcgccgag cacaagcgga
     2941 tgacgcgcga tgccatccgc ggcgtggaga acaacgtgaa gcgcctgtgg ggccagcacc
     3001 agaccctggc cgacccggcc ttcatggatg ccaagcgcgc cgccgagcgc gagctgatcg
     3061 cgagaagcgc gggcaaggcg ggaagcgaac cggtcgccgg cgcggttgcc gccattgccg
     3121 cggtgcagcc gcagcggcgc gcgttgatgc ccttctacga tcagctggag cagttcgccg
     3181 gcgcgtcctc ggtcctctac aactacgccc gcacgctcgt gcgcgccgcg cttgcccgca
     3241 gcgctgctgc tcccggcgat cgcctgccgg agtactcgga cgcccgcctc gcggtcctcg
     3301 gcaagagcct gaccgaaccg cgagccatcg agcccgagct cgaaacgctc tacctcggct
     3361 tctggctgtc gaaggcccgc gaggtgatgg gcaccgatgc gccggagacc aagacgctcc
     3421 tcgggcgcga gtcgcccgag gggctggccg ggcggctggt cgccggcacg aagctcgccg
     3481 atccggccgt gcgcaaggcg ttgtgggacg gcggcgaggc cgccgtccgc gcctcggacg
     3541 atccgctgat ccgcttcgtc ctcgccaccg acgcgcaggc gcgcgccgtt cgtgccgcgt
     3601 gggaggaacg ggcggtcgcc ccgaccgacc gtgccgccga acgcctcgcg ctcgcccgct
     3661 tcgaggcgta cggcgacgat gtctatccgg acgcgacctt cacgctccgg ctgtcgtacg
     3721 gccgcatcga gggctggaag gaaggcgaca gggtcgtcga gccggtgacg cgcatcgccg
     3781 gcctgtggga gcgcgcgacc ggtgccgaac cctacgtagt gtcaccgggc ctggccgcgg
     3841 cccgcgaccg cctcgccccc ggcaccgtct tcaccgtcgc caccaccaac gacatcgtcg
     3901 gcggcaactc gggatcgccg ttgctcgatg cacaggggaa cgtcatcggg gcgatcttcg
     3961 acggcaacct gcactcgaac ggcgggacct acggctacga tccgcagctg aaccgggccg
     4021 tcgccgtttc cgccgccgtc gtacaggagg cgctgctcaa ggtctacgac cagccgcacc
     4081 tggtcaagga actctccgga aagtagaccg gctcaccggc aggttgctga gagggagcgt
     4141 gtgcctttca gcaacctgcc agggcttggg cgagcaccat cacgctggcc cccagcacct
     4201 cttgcaccgg cacccgcctt cgccaaacgc tcgctgcgct cccgccttcg ctgaacgctc
     4261 gctgcgctcc cgccttcgct acccgctcgc tgcgctcccg ccttcgctac
//
LOCUS       DAOPQX010000333         4308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22236_length_4308_cov_3.667059, whole genome shotgun sequence.
ACCESSION   DAOPQX010000333 DAOPQX010000000
VERSION     DAOPQX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22236_length_4308_cov_3.667059"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKK95_14450"
     CDS             complement(<1..1212)
                     /locus_tag="PKK95_14450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC19470.1"
                     /translation="MDKSSVAANTGAQRRATGMFVTADGRNVAVTFVLVSSLFLLWGF
                     CNGMIDVMDKHFQEELHLSLSQSAWVQFAHWMGYFLMALPAGWLATRLGYRGGIIAGL
                     LLVAVGGFWFIPATGIAAFWAFLLGVCMVAAGLTFLETVANPYTTVLGPARYAATRIN
                     LAQSCNGVGWILGPIAGGLFFYSKDATGRSTGSETLWIPYAGVAVLVLLLAVVFYFAP
                     VPDIKAEDERPAEGSDRVAAPGGSSRLVYPLLLGNVTVLLCILGMILWLILGSVGAGG
                     ALLGLWAGVPYARGVIATPESALLATLAAGALAALAIAAVVLQRKRRRIASDSIWCHP
                     HFSGATLAQFFYVAAQAGIFSFLINYMTAEPPGLPRAWLTPATEQWVEVKTAFSASDF
                     KDVSSLAAKLRG"
     gene            complement(1234..2463)
                     /locus_tag="PKK95_14455"
     CDS             complement(1234..2463)
                     /locus_tag="PKK95_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19471.1"
                     /translation="MTHEHATLAAGLTAMTIAVTCLSGASTATFGDDVAFLKRYVDVI
                     VLADRPGSAKIAVTAGWQGRVLTSTARGDDGVSFGWINRELIASRKTLPHMNPFGGED
                     RFWLGPEGGQFSIFFAKGAKFELSDWQTPAPIDTLPFQVAGQSSDSVRFTSAFTLTNY
                     SGTSFDVEVARDVRLLDGASGWKRLGVEAAPGVDLVAYESDNAITNAGKAAWKRETGL
                     LSIWILGMFNPSPATTVVVPIKAGPESELGPKVTSDYFGAVPPERLVVKDDVVFLSGD
                     GRFRSKIGIGPRRSRGVLGSYDGEKQVLTIVQFSQPAGVTDYVNSLWRLQDDPYAGDA
                     ANAYNDGPPAPGAKPLGPFYELESSSPAAALAPGERLSHLHRTIHLTGAEPALDRVAR
                     AVLGVSIADIKGALNAR"
     gene            2622..3959
                     /locus_tag="PKK95_14460"
     CDS             2622..3959
                     /locus_tag="PKK95_14460"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOC19472.1"
                     /translation="MILARREFLMAIGGAAACVRPALAAPAWRAGVASIDITPQPGLW
                     MAGFAARKEPSQGVALPLHAKALALEDARGSRVVIVTLDLLGLTGPVVDRIAAAVERH
                     HRIGRGRLLLCSSHTHSGPVIDDQLAVAYDLSPAQWDAIRTATRRIEAQVVEAVGQAL
                     RSIRPVRLRSGQSSAGFGANRRTAFPGGPSDPTVPVLSVERPDGGPLAVLFGYACHNT
                     TLTADFVRFHGDYAGVAQAEIERRRPGTTALFLAGCGADQNPSPRGSVELVDRHGRAL
                     ADAIDRALGEARPVAGDQLRAAFTTVTLDYAPPPPPDAWRARLEDSNPYVRRHAKLML
                     DAIARDGRVQSSEPAPLHALRIGDLTLVALSGEVVVDYALAIKRAHGDSTWVGGYTDT
                     VFGYLPSVRVLREGGYEGGEAMLYFGRPGPFTESVERTVMTGVEWLLASMRAS"
     gene            complement(4027..>4308)
                     /locus_tag="PKK95_14465"
     CDS             complement(4027..>4308)
                     /locus_tag="PKK95_14465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19473.1"
                     /translation="RSNRLRFTPDGRLVLVSDLEAGDLVVIDRASRRVTKRMPLGRAP
                     SGILVVPDGSRAYVALTGENALAIVDLKTLEVAGRIATGEGPDGMAWRQ"
BASE COUNT          652 a         1518 c         1534 g          604 t
ORIGIN      
        1 tccgcgcagc ttcgcggcca gcgatgaaac gtccttgaaa tccgaagccg agaacgccgt
       61 cttcacctcg acccactgct cggtggccgg cgtgagccac gcccgcggca ggcccggcgg
      121 ctcggccgtc atgtagttga tgaggaagct gaagattccc gcctgggcgg cgacgtagaa
      181 gaactgcgcc agcgtcgcgc ccgagaagtg cgggtggcac cagatgctgt cgctcgcgat
      241 ccggcgcctc tttcgctgca gcaccacggc cgcgatcgcc agggcggcga gcgcgccggc
      301 cgcgagggtc gccaggagcg cgctctccgg cgtggcgatg acgccgcgcg cgtagggaac
      361 gcctgcccac agcccgagca gcgccccgcc ggcccccacc gaacccagga tcaaccagag
      421 gatcatgccg aggatgcaca gcaggaccgt gacgtttccc agcagcagcg gatagaccag
      481 ccggctgctc ccgcccgggg ccgcaacccg gtcgctgccc tcggcggggc gttcgtcctc
      541 ggccttgatg tcgggcaccg gagcgaagta gaagaccacg gccaacagga gtaccagcac
      601 cgcgacgccg gcgtacggaa tccacagcgt ctcgcttccg gtgctcctgc cggtggcgtc
      661 tttcgagtag aagaacaggc cgccggcaat cgggccgagg atccagccga cgccgttgca
      721 ggactgggcg aggttgatcc gcgtcgccgc gtagcgggcc gggccgagca cggtggtgta
      781 cgggttggca accgtctcca ggaacgtgag ccccgcggcc accatgcaga cgccgagcag
      841 gaaggcccag aacgccgcga tgcccgtcgc ggggatgaac cagaagccgc ccaccgccac
      901 cagcaggagg ccggcgatga tcccgcccct gtagcccagg cgcgtggcca gccacccggc
      961 cggcagcgcc atgaggaagt agcccatcca gtgcgcgaac tgcacccagg ccgactgcga
     1021 caggctcagg tgcagctcct cctggaagtg cttgtccatc acgtcgatca tgccgttgca
     1081 gaatccccag aggaggaaca gggaactgac gagcacgaag gtgacggcca cgttccggcc
     1141 gtccgccgtg acgaacatgc ccgtggcccg gcgctgtgca cccgtgttgg cggcgacgct
     1201 cgacttgtcc atggtgtggt gtgcccggcg acgttaccgc gcgttcagcg cgcccttgat
     1261 gtccgcaatc gagaccccga gcacggcccg ggccacgcgg tcgagcgcgg gctcggcgcc
     1321 ggtcaggtgg atggtccggt gcaggtgtga gaggcgttcg cccggggcca gcgcggcagc
     1381 gggcgacgaa gattccagct cgtagaacgg gccgagcggc ttggctcccg gcgccggcgg
     1441 accgtcgttg taggcgttcg ccgcgtcgcc cgcgtacgga tcgtcctgca gccgccacag
     1501 cgagttcacg tagtcggtga cgccggccgg ctgactgaac tgaacgatcg tgagaacctg
     1561 tttctcgccg tcgtagctgc cgagcacgcc cctgctgcgc cggggcccga tcccgatctt
     1621 gctgcggaac cgtccgtcgc cgctgaggaa gaccacgtca tccttgacca cgagccgctc
     1681 cgggggcacc gcgccgaagt agtcggaggt gaccttcggc cccagctccg actcggggcc
     1741 cgccttgatc ggcaccacga ccgtggtggc gggagagggg ttgaacatcc ccaggatcca
     1801 gatggagagc aggccggtct cacgcttcca cgcggccttg ccggcgttgg tgatcgcgtt
     1861 gtccgactcg tacgcgacca ggtccactcc cggcgccgcc tccaccccga ggcgcttcca
     1921 gcctgacgcg ccgtcgagga gccggacgtc ccgcgccacc tcgacgtcga aggaggtgcc
     1981 cgagtagttg gtcagcgtga aggccgaggt gaacctcacc gaatcggacg actggccggc
     2041 gacctggaac ggcaaggtgt ctatcggggc gggcgtctgc cagtctgaga gctcgaactt
     2101 cgcgcccttg gcgaagaaga tcgagaactg tcccccctca gggcccagcc agaagcggtc
     2161 ctcgcccccg aatgggttca tgtgcggcag ggtcttgcgc gaggcgatga gctcccgatt
     2221 gatccagccg aagctcacac catcgtcgcc gcgtgcggtg ctcgtcagca cccggccctg
     2281 ccagcccgcg gtgaccgcaa tcttggccga gccgggccga tcggccagga cgatcacgtc
     2341 gacgtaccgt ttcaaaaacg cgacgtcgtc cccgaaggtg gcggtggagg ctccagagag
     2401 acaggtgacg gcgatggtca tggctgtgag tcccgcggcg agggtggcgt gttcgtgtgt
     2461 catgtccgca ccagatggat gtcacgggcg ccggccggaa ggccggccgg cctgggtccc
     2521 ggcgcatggt agccgaagtc gccgccacga gaccagcgcg gggcgccccc aggggtaaga
     2581 tactgctggt ccggagatgg cccggacggg aaggtgaccc gatgatcctc gcgcgtcgcg
     2641 agttcctgat ggcgatcggc ggcgcagccg cgtgcgtgcg cccagccctg gccgctcccg
     2701 cctggcgcgc cggcgtggcg tcgatcgaca tcacgccgca accggggctc tggatggccg
     2761 ggttcgctgc gcgcaaggag ccctcgcagg gagtggccct gccgctgcac gcgaaggcgc
     2821 tggcgctgga ggacgcgcgg ggcagccggg tggtcatcgt gacgctcgac ctgctcggac
     2881 tcaccgggcc ggtcgtggac cgcatcgccg cggccgtcga gcgccatcac cgcatcgggc
     2941 gcggccgtct cctgctctgc agcagccaca cgcacagcgg cccggtcatc gacgaccagc
     3001 tggccgtggc ctacgacctg tcgcccgcgc agtgggacgc gatccgcacg gccactaggc
     3061 ggatcgaggc gcaggtcgtc gaggcggtcg ggcaggcgct ccggtcgatc cgtcccgtgc
     3121 gcctgcggtc gggacaatcg agcgccgggt tcggcgccaa ccgccgcacg gccttcccgg
     3181 gcggccccag cgatccaacc gtgccggtgc tctcggtcga gcgtcccgac ggcgggccgc
     3241 tcgcggtgct gttcggctac gcctgccaca acaccaccct caccgccgac ttcgtgcgct
     3301 tccacggtga ttacgccgga gtggcccagg ccgagatcga gcgccggcgt cccgggacga
     3361 cggcgctgtt cctcgcgggc tgcggcgccg accagaaccc gtcgccccgc ggcagcgtcg
     3421 aactggtcga tcggcacggc cgggctctcg ccgacgcgat tgaccgcgcc ctcggcgagg
     3481 cgaggcccgt cgccggcgac caactgcggg ccgccttcac gacggtcacc ctcgactacg
     3541 ccccgccgcc gcccccagac gcctggcgcg cacggctcga ggactcgaat ccttacgtcc
     3601 gccggcatgc gaagctgatg ctcgacgcca tcgcccggga cgggcgcgtc cagtcctcgg
     3661 agcccgcgcc gctgcacgcg ctccggatcg gagacctgac cctggtggca ctcagcggcg
     3721 aggtggtggt ggactacgcg ctggcgatca agcgggcgca cggcgactcg acctgggtgg
     3781 gcggctacac cgacacggtg ttcggctacc tgccgtcggt tcgcgtgctg cgcgaggggg
     3841 gctacgaggg gggcgaggcc atgctgtact tcggccggcc cgggccgttc accgagagcg
     3901 tcgaacggac ggtgatgact ggcgtcgagt ggctgctggc gtcgatgcgc gcctcgtgat
     3961 cgcgcctgtc aggggtcgct cgcgcagggc ccaggtcggg ttcagcctgc cgaatcagcg
     4021 cggcgctcac tggcgccagg ccatgccgtc gggcccctcg ccggtggcaa tccgcccggc
     4081 cacttccagt gtcttcagat ccacgatggc cagggcgttc tcgcctgtca gggccacgta
     4141 ggcgcgcgag ccgtcgggca cgacgaggat gccggacggc gcgcggccga gcggcatccg
     4201 cttggtcacg cggcgcgagg cgcggtcgat cacgaccagg tcgcctgcct cgagatccga
     4261 aaccagcacg aggcggccgt ccggggtgaa gcggaggcgg ttggatcg
//
LOCUS       DAOPQX010000334         4301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22275_length_4301_cov_5.348092, whole genome shotgun sequence.
ACCESSION   DAOPQX010000334 DAOPQX010000000
VERSION     DAOPQX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22275_length_4301_cov_5.348092"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKK95_14470"
     CDS             complement(<1..480)
                     /locus_tag="PKK95_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19474.1"
                     /translation="MNRKISTVLAAAVAAGLLAAVTLAAQGVPAGLANADLQQRAVPS
                     GLEQAFSGLVAARQAPGWVAWSVAAIPGDHRSCDAGRDDGVVHLESARRRAGGRSRVT
                     LEGPSEILVFIRAEQRRVQKIRVISPDCEIDAGGLPVHWLTGVNPSESVTLLASFARD
                     "
     gene            complement(534..1946)
                     /locus_tag="PKK95_14475"
     CDS             complement(534..1946)
                     /locus_tag="PKK95_14475"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOC19475.1"
                     /translation="MVKHSILVGAAIVVMAASAAAQTPAPVPPLPPAPPHPPLAVVAE
                     ALADLEFDFDLDFDIAPALAGTNAALASLEVLGLQEPGTKESAKLAREMAEASREVDQ
                     ATREYNRATRALDRARYEEALQAFEKVAAMGGRRADGALYWQAYALSRLGQRAEALAK
                     LQDLQKKFPSSRWLNDARALELAMKTASGQTVSPQAQDEELKAMALRSVMLNDPDRAV
                     PMVEEMLKGASSPKVKEQALFVLAQSGTPQASEILARVARGQGNPDLQLKAVRYLAMF
                     GKPENRSVLAEIYRSSPDVEVKRHVMQAFMMAGDRDRLLEAAKSEKSPELRAEAVRQL
                     GVMGAQAELAGLYGTEVSAEVRRQVLQAMAISGASDKLAALAKAEADAALRRTAVRNL
                     GMMDSKKTGDVLAQFYRDDKDPGVRKAAIQGLFMQGNAGALVALARQEKDPELRKEIV
                     SRLSMMKNKEAMDYMMELLK"
     gene            complement(2030..2899)
                     /locus_tag="PKK95_14480"
     CDS             complement(2030..2899)
                     /locus_tag="PKK95_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOC19476.1"
                     /translation="MRHPTDEQFVLYHYGELDEAEGAAFRSHIRECEGCQRQLATLGR
                     ELAAVRPVSIPERPADYGARVWARLQPRLADQRARESKPARRWWPFGAVALRPLAYAA
                     GLAVLVVAAFVAGRYWAPAPAPEQVASGTPASSTPSTEPGSEPELAGAPPGAQGSTAA
                     AAVAGVRERILVLEVGEHLERSRAALLEVMNAPDEDGAIDLSADQERVRDLVAENRLY
                     RLSAIDAGENGMAAVLDDLERALVEIANGPSRVSETEFEHVRGEIDAQGLIFKVGILG
                     ETLRQRQAKGSSI"
     gene            complement(2896..3483)
                     /locus_tag="PKK95_14485"
     CDS             complement(2896..3483)
                     /locus_tag="PKK95_14485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC19477.1"
                     /translation="MDATDTPDVALAQSGDESAFQRLVERHSVGVFQLAFRLTGNESD
                     AEDVVQDTFLKVYRELKRFESRSSFKTWLHRVTVNCSYDLLRKRPRVRLEPIEADDGE
                     ARWRAEPEADAASRPDRVAYGSEVHRRIEAAMQRLSPAERTAFVLRHFEGHSLEEIGE
                     ALGLRLGATKHSVFRAVQKMRRALAPVARTAEWGS"
     gene            complement(3547..3822)
                     /locus_tag="PKK95_14490"
     CDS             complement(3547..3822)
                     /locus_tag="PKK95_14490"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOC19478.1"
                     /translation="MTNDPDAPVHLPIEPALDLHPFAPRDVKSVVEEYVTAAWEAGLR
                     EVRLIHGRGRGVQRGIVQAALERHPLVAEFWDATETHLGATVARLVD"
     gene            complement(3903..4244)
                     /gene="queF"
                     /locus_tag="PKK95_14495"
     CDS             complement(3903..4244)
                     /gene="queF"
                     /locus_tag="PKK95_14495"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOC19479.1"
                     /translation="MPPPIETFPNPKPDRDYEIAIDCPEFTSVCPKTGLPDFGTIRIT
                     YVPGERCLELKALKYYLLEFRSRGIFYEQVTNQILDDLVASCAPRRMTVVGDFTPRGG
                     IKTVVTARYEE"
BASE COUNT          595 a         1528 c         1489 g          689 t
ORIGIN      
        1 gtcgcgggcg aaggaggcga gcagcgtcac gctctcgctt ggattgaccc cggtcagcca
       61 gtggaccggg aggccgcccg cgtcgatctc gcagtcgggg gagatgacgc gaatcttctg
      121 gacgcgccgc tgctcggccc ggatgaagac caggatctcg gacgggcctt cgagggtgac
      181 gcgggatcgg ccgcccgcac ggcggcgcgc cgattcgaga tgcaccaccc cgtcgtcacg
      241 cccggcgtcg caggagcggt ggtcgccggg gatcgcggca accgaccagg cgacccaccc
      301 cggcgcctgc ctggcggcca cgaggccact gaacgcctgc tcgaggccgg acggaacggc
      361 gcgctgctga aggtcggcgt tcgcgagacc cgcgggaacc ccttgcgctg ccagggtgac
      421 cgccgcgagc aggcccgcgg cgacagctgc cgccaacacg gtcgaaatct tgcggttcat
      481 gggtggcctc tctggggttg ggggccgggg tagggcgacc acaagggtcg cccctacttc
      541 agcagctcca tcatgtagtc catcgcctcc ttgttcttca tcatcgagag gcgggagacg
      601 atttccttgc gaagctcggg gtccttctcc tggcgggcga gggcgacgag ggcgccggcg
      661 tttccctgca tgaacagccc ctggatcgcg gccttcctga cgcccgggtc cttgtcgtcg
      721 cggtagaact gggcgagcac gtcgccggtc ttcttcgagt ccatcatccc gaggttgcgg
      781 accgcggtcc gccgcagggc ggcgtcggcc tcggcctttg cgagcgccgc gagcttgtcg
      841 ctggccccgc tgatggccat cgcctggagc acctgccggc gcacctcggc cgaaacctcg
      901 gttccgtaga ggcctgccag ctcggcctgc gcacccatga ctcccagttg ccgcacggct
      961 tcggctcgca gctcgggcga cttttcgctc ttcgccgcct cgagcagccg gtcgcggtcg
     1021 cccgccatca tgaacgcctg catgacgtgg cgcttcactt cgacgtccgg ggaagagcgg
     1081 tagatctcgg cgaggacgga ccggttctcc ggcttgccga acatcgccag gtagcgcacc
     1141 gccttgagct ggaggtcggg atttccctgg cctcgggcga cccgcgcaag gatctcgctg
     1201 gcctgcggcg ttccgctctg ggccagcacg aagagcgcct gttccttcac cttcggcgag
     1261 ctggcgccct tcagcatctc ctcgaccatc ggcaccgcgc ggtcggggtc gttgagcatc
     1321 accgagcgga gggccatcgc cttcaattcc tcgtcctgcg cctgcggcga gaccgtctgc
     1381 cccgaggcgg tcttcattgc cagctcgagc gccctggcgt cgttgagcca acggctcgac
     1441 gggaacttct tctgcaggtc ctgcagcttg gcgagcgcct cggcgcgctg gccgagccgg
     1501 ctgagcgcgt aggcctgcca gtagagggcc ccgtcggccc ggcggccgcc catggccgcc
     1561 accttctcga aggcctggag cgcctcctcg taccgggcgc gatcgagggc acgggtggcg
     1621 cggttgtact cccgcgtcgc ctggtcgacc tcgcgcgagg cctcggccat ctcgcgcgcc
     1681 agcttggcgg attccttggt gccgggctcc tgcaggccga gaacctcgag gctggccagc
     1741 gcggcgttgg tgcccgccag cgcgggagcg atgtcgaagt cgaggtcgaa atcgaactcg
     1801 aggtcggcca gcgcctccgc cacgaccgcg agcggaggat ggggcggcgc gggcggaagc
     1861 ggcggcaccg gcgcgggcgt ctgcgccgcg gccgacgccg ccatcaccac gattgccgcg
     1921 ccaacgagaa tcgaatgttt caccataaat gtctccaact cctgtccggc ccgcttgggt
     1981 cctcggcaag cccttgacct tgagccctga gccctgagcc gtgagccttc tagatgctgc
     2041 tccccttggc ctgccgctgc cgcagcgtct cgccgaggat cccgaccttg aagatcagtc
     2101 cctgtgcgtc gatctccccg cgaacgtgct cgaactcggt ctccgacacg cgggacgggc
     2161 cgttggcgat ctcgaccagc gcccgttcga ggtcgtcgag cactgcagcc atcccgttct
     2221 ctcccgcgtc gattgccgaa agccggtaga ggcggttctc ggcgacgaga tcgcggacgc
     2281 gctcctggtc ggccgacagg tcgatcgccc cgtcctcgtc cggcgcgttc atcacctcga
     2341 gcagggccgc ccgcgagcgc tcgaggtgtt cgccgacctc gagcacgagg atccgctcgc
     2401 ggacaccggc gacggccgcc gccgcggtcg agccctgcgc accaggcggc gcgccggcca
     2461 gctccggttc cgagccgggc tccgtggaag gcgtcgagga ggcgggcgtg ccgctcgcta
     2521 cctgctccgg ggcgggcgcc ggtgcccaat accgcccggc gacgaacgcc gcaaccacga
     2581 gcacggccag ccccgcggcg taagccaggg ggcggagcgc gacggccccg aagggccacc
     2641 accgcctcgc cggcttcgac tcgcgagccc gttggtcggc cagccgcggc tgcagccgcg
     2701 cccagacgcg ggcaccgtaa tcggccgggc gttcagggat ggacactggc cgcacggccg
     2761 ccagctcgcg tccgagcgtg gccagctgcc gttggcatcc ctcgcactcc cggatgtgcg
     2821 agcggaaggc ggcgccctcc gcctcgtcca gctcgccgta gtggtagagg acgaactgct
     2881 cgtcggtcgg gtgtctcacg atccccactc cgccgtcctg gcgacgggcg ccagcgcccg
     2941 ccgcatcttc tggacggccc gaaagacgct gtgcttggtg gcgccgagcc ggaggcccag
     3001 cgcctctccg atctcctcga gcgagtgccc ctcgaagtgc cgcagcacga acgcggtgcg
     3061 ctcggccggc gagaggcgct gcatggccgc ctcgatccgg cggtggacct ccgagccata
     3121 ggccacgcgg tcgggccggc tcgcggcatc ggcctccggc tcggcgcgcc agcgggcctc
     3181 cccgtcgtct gcctcgatcg gttccagccg cacgcggggc cgcttgcgga gcaggtcgta
     3241 cgagcagttc acggtcaccc ggtgcagcca ggtcttgaag ctcgacctcg actcgaaccg
     3301 cttcagctcc cggtagacct tgaggaaggt gtcctgcacg acgtcctccg cgtccgactc
     3361 gttcccggtg agacggaagg cgagctggaa cacgcccacg ctgtggcgtt cgaccagtct
     3421 ctggaaggcc gattcgtcgc cggactgggc cagggccacg tccggcgtgt ccgtcgcatc
     3481 catctggcta cttagacggg gggcggcgaa tttggttagt ccacgaccgg gcgtgcgttg
     3541 cccggttcaa tcgaccagcc gggcgacggt ggctccaagg tgtgtctcgg tggcgtccca
     3601 gaactcggcg accagggggt gacgctcgag cgctgcctgg acgatgccgc gctggacgcc
     3661 gcggccccgc ccgtgtatca ggcgcacctc gcgcagcccc gcctcccacg cggccgtcac
     3721 gtattcctcg accaccgact tcacgtcgcg gggcgcgaag gggtgcaggt cgagcgccgg
     3781 ctcgatgggg aggtggaccg gagcgtcggg atcattcgtc acgggcaacc gccagtccag
     3841 acgtccgact cgacttcgcg acttcgtgac ctctccgacc tcgtgacttc gtggactgcc
     3901 cgtcactcct cgtaccgggc cgtcaccacc gtcttgattc ccccgcgggg cgtgaagtcg
     3961 cccacgaccg tcatccggcg gggcgcgcag gaggccacca ggtcgtcgag gatctggttg
     4021 gtgacctgct cgtagaagat cccgcggctg cggaactcga gcaggtagta cttcagcgcc
     4081 ttcagctcga ggcagcgctc gccgggaacg taagtgatgc ggatggtgcc gaagtccggc
     4141 agccccgtct tggggcagac ggaggtgaac tcggggcagt cgatcgcgat ctcgtaatca
     4201 cggtcgggct tcgggttggg gaaggtctcg atcgggggtg gcatggtccg attctacgct
     4261 ggcgatctgt gcgggaaccc gcgaaaatgc ttgcgatcca a
//
LOCUS       DAOPQX010000335         4290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22341_length_4290_cov_4.404014, whole genome shotgun sequence.
ACCESSION   DAOPQX010000335 DAOPQX010000000
VERSION     DAOPQX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22341_length_4290_cov_4.404014"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKK95_14500"
     CDS             <1..319
                     /locus_tag="PKK95_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19480.1"
                     /translation="QQQQQKPAAVDDDQEAQMDEALKKFGYVSGQAGACQTPGPGRTK
                     HERQALDVATGVLRLFGSDRAFYYAAAYGAGVTAPSDRAKCPETIKEYERMLAKVKAL
                     AAR"
     gene            335..619
                     /locus_tag="PKK95_14505"
     CDS             335..619
                     /locus_tag="PKK95_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19481.1"
                     /translation="MRLGWTGRCGLAFLAILLVSPAARADRWDVLREIGEGDREIRRE
                     RREAVREILRADSGWEMAREIGEGAREIRREKREKRREVRREIRVELFRR"
     gene            720..2309
                     /locus_tag="PKK95_14510"
     CDS             720..2309
                     /locus_tag="PKK95_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOC19482.1"
                     /translation="MAVAPVVLDGVVLFRVRGVSSLPAETRARLIGDRIAEVAADRTI
                     EPGSLHVVEGEGVSRIVARGVPVVTLVDADAALEGLHRKELASAHLAKVQEAIADYRA
                     ARTPGAMRRSIVNAVGAGLVLALAVAALAWSWRRVDRFLERRLKEGVRSVEIRSFRLM
                     RGEAVWRAARSVLVALRAVVLLGILLLFLSFVLVQFPWTRGLSRGMAAFALRPLQEMG
                     EGLLSSIPSLVFLAVLFLAVRLGLRAVRLFFDAVGRGAVTLQGFDREWAEPTYKIIRI
                     AAIAFALIVAYPYIPGSDTAAFKGVSVFIGVVFSLGSSSVVSNIIAGYMMTYRRALKV
                     GDRVRIGDAVGDVIETRLQVTHLRSPKNEEIIVPNSQILAGEVTNYSSLARVHGLILH
                     TEVGIGYDTPWRQVEAMLLLAAERTEGVAREPRPFVLEKKLGDFAVVYELNAYCDNPQ
                     AMNLLYAALHRNVLDVFNEYGVQIMTPAYEGDTPEPKVVAPNDWFAAPADTTPSDTAP
                     AGTSRLQPSRRTATGQPTPHS"
     gene            2539..>4290
                     /locus_tag="PKK95_14515"
     CDS             2539..>4290
                     /locus_tag="PKK95_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19483.1"
                     /translation="MHRLLHALRTGCVVVTAVLGLSAAWTSPAAAQAVPPTDPFLGVP
                     ILTSGASTPGGTVTVVNDTSRATTAGGAHALVGNASAGGYPQSVIDSLTAFSAAPFSF
                     QLTRVWNADSTEGTVGDPATGTRLDVEGNPLPGLGGSFTLNADGTFTFNPGTDFDDLP
                     NGSSVLTTVRYEVTGTNNTPTGDGSPNPIRDFAGLLIVEIERNAADGTLSCAPAGADF
                     ADFGSFSLPGSAFPDLKAYVDSLLRFRYSGGPGSDVVITNTGPLETGGDEAHGIYARA
                     SGQRGTDGRNASFARSAHPGGGGGAGGDITVSSEASITTSGYRSAGIVATSIGGDGGT
                     GGEAAWWWDAKSGGAGGDGGSVMVSGAGTIDTAGEGAVGVLALSQGGRGGDGGDGATF
                     WGADNGAKGGAGGTVIVDGGWNIVTSGKDAHGIWARSLGGVAGSGGDGGWTGTDAGGG
                     GQGTHGGEVTVVSRGSIHTSGESSYGIFAQSIGGFGGSGGEGGSLFYASGGNGAGAGS
                     GGNVSVTNEGSIDTSGAAAHGIFAQSIGGGGGSGGSGLALIGFGGDSASGGNGGTVVV
                     TNRSTITATGAGSRGIYA"
BASE COUNT          630 a         1465 c         1563 g          632 t
ORIGIN      
        1 gcagcagcag cagcagaagc cagctgccgt tgacgacgac caggaagcgc agatggacga
       61 ggccctcaag aagttcggct acgtctccgg ccaggcgggt gcctgtcaga cgcccggacc
      121 cggccggacg aagcacgagc gccaggccct cgacgttgcg accggcgttc tccggctgtt
      181 cggatcggac cgggcgttct attacgcggc ggcgtacggt gcgggggtga ccgccccctc
      241 cgaccgcgcc aagtgccccg agacgatcaa ggagtacgag cggatgctgg ccaaggtgaa
      301 ggcgctggcc gcacggtgaa cgcaggagga actcatgaga ttgggatgga caggacggtg
      361 cggcctcgcg ttcctggcga tcctgctcgt ctcgcccgcc gcgcgggccg atcgctggga
      421 cgtcctgcgc gagatcggcg agggcgatcg ggagatcagg cgggagcggc gcgaggccgt
      481 gcgcgagatc ctgcgtgccg actcggggtg ggagatggcc cgcgagatcg gggagggagc
      541 ccgtgagatc cggcgcgaaa agcgcgagaa gcggcgcgag gtgcggcgcg agatccgcgt
      601 cgaactgttc cgccgctagc cggcgggctg cgctcgccct cgtcctgctg acctcggtcg
      661 cgacggtctc gcaggcaccg ctgaccgcgc aggaggcggg cgccgggccc gagccacatg
      721 tggccgtggc gcccgtcgta ctggacgggg tcgtcctctt ccgcgtgcgg ggagtctctt
      781 cgctgccggc ggagacgcgc gcgcgcctca tcggggatcg cattgccgag gtcgccgcgg
      841 atcgcacgat cgagccgggc tcgctccatg tcgtcgaggg cgagggggtc agccggatcg
      901 tggcccgcgg cgtgccggtc gtcaccctcg tggatgccga cgccgcgctc gaggggctcc
      961 atcggaagga actggcctcc gcgcaccttg ccaaggtgca ggaggcgatc gcggactacc
     1021 gggcggcccg cactcccggc gcgatgcggc gcagcatcgt gaatgcggtc ggggccgggc
     1081 tcgtcctcgc gctcgccgtc gcggcgctcg cgtggtcgtg gcgccgggtg gatcgtttcc
     1141 tcgagcgccg cctgaaggag ggcgtgcgtt cggtcgagat ccggtccttc cgcctgatgc
     1201 gcggcgaggc ggtgtggcgg gcggcgcgga gtgtcctcgt cgcgcttcgg gcagtcgtcc
     1261 tgctcgggat cctcctcctg ttcctgagct tcgtgctggt ccagtttccc tggacgcgcg
     1321 ggctctcccg cggcatggcc gccttcgcgc ttcggccgct gcaagagatg ggcgaggggc
     1381 tcctgtccag catcccgagc ctggtgttcc tcgcggtgtt gttcctcgcg gtccggctgg
     1441 gactcagggc ggtgcgcctg ttcttcgacg cggtgggacg aggggcggtc accctgcagg
     1501 gcttcgatcg cgaatgggcc gaacccacct acaagatcat ccggatcgcg gcgatcgcct
     1561 tcgcgctcat cgtcgcctac ccctacatcc cgggctccga caccgccgcc ttcaaggggg
     1621 tgtcggtgtt catcggcgtg gtgttctcgc tcgggtcgtc atcggtggtc tcgaacatca
     1681 tcgccggtta catgatgacc taccggcgcg cgctcaaggt gggcgatcgg gtcaggatcg
     1741 gcgatgcggt cggggacgtg atcgagacgc ggctccaggt gacccacctc cgctcgccga
     1801 agaacgagga gatcatcgtc cccaattcgc agatcctcgc cggcgaggtg accaactaca
     1861 gctcgctggc acgggtgcac gggctgatcc tccacaccga ggtggggatc gggtacgaca
     1921 cgccgtggcg gcaggtggag gcgatgctcc tgctggcggc cgagcgcacg gagggtgttg
     1981 cccgggaacc gcgcccgttc gtgctcgaga agaagcttgg tgacttcgcg gtcgtctacg
     2041 agttgaacgc ctactgcgac aacccccagg ccatgaacct gctctacgcc gcgctgcacc
     2101 ggaacgtcct cgacgtgttc aacgagtacg gcgtccagat catgaccccc gcgtacgaag
     2161 gcgacacgcc ggagccgaag gtcgtggcac cgaacgactg gttcgccgcg cccgccgaca
     2221 caaccccctc cgacacggcg cctgccggca catcgcgcct gcagccttcc cggcgcacgg
     2281 ccaccggtca gccgacgcct cattcgtgag aatgacgatc gcgtcgcgcc ctcgtctcag
     2341 ccttctcccg tcgtttccga tggcgccgtg cagggcaagg tgatacgctg gcgtcacttc
     2401 tcgagttcca cgcctccgac aacgatggca ccaaggcaat ctcgtagtgg tgtgccgttc
     2461 ttctcactga ccccttcagc cgcaatccct tcgccggccg acccctttcc ggaccagacg
     2521 cccccggaga tccactcaat gcaccgcctc cttcacgcac tccgcaccgg ctgcgtcgtc
     2581 gtgaccgcgg tactgggcct gtccgctgcg tggacgagcc cggccgccgc gcaggccgtg
     2641 ccgccgacgg acccgttcct gggcgtgccg atcctgacga gcggggcttc gacgcccggc
     2701 ggcacggtga cggtcgtgaa cgacacctcc agggcgacca cggctggcgg ggcccacgcg
     2761 ctggtgggca acgcgtcggc cggcggctat ccacagagcg tgatcgacag cctgacggcc
     2821 ttttccgccg ccccgttctc attccagctc acgagggtct ggaacgccga ttcgaccgag
     2881 ggaacggtcg gtgatcccgc aacgggcacg cgtctcgatg tcgaaggcaa ccccctcccc
     2941 ggcctcggcg gatccttcac gctgaacgcg gacgggacgt tcacgttcaa tccaggcacc
     3001 gacttcgacg atctgccgaa tggatcgtcc gtcctcacca ccgtccggta cgaggtcacg
     3061 gggacgaaca acaccccgac cggcgacggg tcgccgaacc cgatcaggga cttcgccggc
     3121 cttctcatcg tcgagatcga gaggaacgcc gcggacggca ccctgagttg cgcgccggcc
     3181 ggcgccgact tcgccgactt cggatcgttc agcctgccgg ggagcgcctt tcccgatttg
     3241 aaggcctacg tggacagcct gctccgcttc cgctactcgg gtgggccggg cagcgacgtc
     3301 gtgatcacca acaccggccc gctcgaaacc gggggcgacg aggcgcacgg catctacgcc
     3361 cgggcgtcgg gccagcgggg gaccgacggc cgcaacgcga gcttcgcgcg ctcggcccac
     3421 ccgggcggcg gaggtggcgc cggaggcgac atcaccgtgt cctccgaagc cagcatcacc
     3481 acgtcggggt accgatcggc cggcatcgtc gccaccagca tcggcggcga cggagggacg
     3541 ggcggcgaag cggcgtggtg gtgggacgcc aagtccggcg gcgctggcgg ggacggcggc
     3601 agcgtgatgg tgtccggcgc cgggaccatc gacacggccg gcgagggtgc tgtaggcgtg
     3661 ctcgcactca gtcaaggggg gcgcggcggc gacggcggcg acggtgccac cttctggggc
     3721 gccgacaacg gcgccaaggg cggtgcaggc ggcaccgtca tcgtggacgg cggctggaac
     3781 atcgtcacga gcgggaagga cgcccacggc atctgggcca ggagcctggg cggcgtcgcc
     3841 ggcagcggag gtgacggcgg atggacggga accgacgcgg gcggcggcgg ccagggcacg
     3901 cacggcggcg aagtgaccgt ggtgagccgc ggcagcattc acaccagcgg cgagagctcg
     3961 tacggcatct tcgcgcagag catcggcggc ttcggcggca gcgggggcga gggcggcagc
     4021 ctgttttacg cgtcgggcgg caacggcgcg ggggccggca gcggcgggaa cgtctcggtg
     4081 accaacgagg gctcgatcga cacctcgggg gccgccgcgc acggcatctt cgcgcagagc
     4141 atcggcggcg gcggcggcag cggcggcagc gggttggcgc tcatcggctt cggcggcgac
     4201 agcgcgtcgg gtggaaacgg cggcacggtc gtcgtgacga acaggagcac catcacggcc
     4261 accggcgccg gctcgcgggg catctacgcc
//
LOCUS       DAOPQX010000336         4277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22414_length_4277_cov_3.664377, whole genome shotgun sequence.
ACCESSION   DAOPQX010000336 DAOPQX010000000
VERSION     DAOPQX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22414_length_4277_cov_3.664377"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKK95_14520"
     CDS             complement(<1..385)
                     /locus_tag="PKK95_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOC19484.1"
                     /translation="MLERRLYYHIDWLLIAAIAALSAMGVAMIYSTTHAGANAGLYVR
                     QLYAIGLGAFVFMVCLTIDYRTLADNSLAIYGLIVALLVGVLLFGTTGGGARRWIDLP
                     FFNLQPSEFAKVALALALAKFFDESR"
     gene            complement(414..2324)
                     /gene="mrdA"
                     /locus_tag="PKK95_14525"
     CDS             complement(414..2324)
                     /gene="mrdA"
                     /locus_tag="PKK95_14525"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOC19485.1"
                     /translation="MRLLAPAEPSVSRPQDRRKITVRLNGIQAAITVVFAVLALAFWY
                     FQVVQHAKFRELAENNHQRTLSLRAPRGVLYDRHGAVLVENRDSFVISLVREHIKDAD
                     ETIRLLCRVAQLDEAAVRETVRRNRNLPAYRPIPVVEDASLAQVAAVMARRLDTELPG
                     VIVERVPTRRYPSESLAAHLFGYVGQATEEQVQAGGLQPGDIVGQSGIERVYNHLLMG
                     QDGARRVVVNSTGREIRTLEEIPPSEGRRVQLTLDYDLQRAAEEGFRGYGYAGAAVVL
                     DPRSGEVLAYVSLPAYDPNAFAGGIDRVTWNQLNTDKLRPLQNRAIQGRYSPGSTFKI
                     AVATAALEEGLVTPDFRVHCGGGATFYGRYFQCWEKRGHGSVDMRHAIERSCNVYFYT
                     LGNMVGIDRLHKWATLLGLGEKSGIDLPNEIDGIMPSTAWKQARFGEKWYSGETISVA
                     IGQGYVSVTPIQLAVMMSTVANGGTRVTPHLVRAEDAGGGWKPVPAPEPKSRVAFKPE
                     TVAALHDGLWMVVNGAGTGGRARIQGRDVSGKTGTAQVISLQGGRAAAGRTEMDLRDH
                     GWFVFFAPRDNPEIAGAVFAEHSEHGYLAAPIAKYVMETYFAKKEGRPLPTFTPPAAP
                     APAVATIATAGQ"
     gene            complement(2321..2830)
                     /gene="mreD"
                     /locus_tag="PKK95_14530"
     CDS             complement(2321..2830)
                     /gene="mreD"
                     /locus_tag="PKK95_14530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOC19486.1"
                     /translation="MTALRVLVTIAAALVLQTTLARFLVRGAGGVDLVLVAVVYLGLT
                     SGPTAGILSGTIAGLAQDSLGSGIVGVGGLAKTVVGYLAGRIGTTFIVSQTIPRFLVF
                     FGATLVQVGLTTGLYYLLDPQPPVVTLYAALGESLANALLGVVFFQVLDALPGMIDRR
                     RGMSRGRVR"
     gene            complement(2827..3684)
                     /gene="mreC"
                     /locus_tag="PKK95_14535"
     CDS             complement(2827..3684)
                     /gene="mreC"
                     /locus_tag="PKK95_14535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOC19487.1"
                     /translation="MTNFSGIRGRSGYLFVAAAVGHIILISAQVNTRSGVPVLQAVLF
                     GALAEVQRATSGVMDATHRGWEGYVRLGDAQAENERLRHEVAELRVQLQLARAEAGRT
                     TRMRQLLDLRDRTSLPTRAAEIIGTSATTDFKTVTIDRGTDHGVLTDMAVVSPGGAVG
                     RVVMPTRHAAKVQLLIDRSAAVAVVVERSRAQGVAFGTGDTTLRLEYLPTTADITEGD
                     VLTTSGIDGIYPPGFLVGRVERIERAAGVFKSIRVAPSVDFGSLEAVLVVVAPSGQTG
                     VSEARGVSR"
     gene            complement(3677..>4277)
                     /gene="mreB"
                     /locus_tag="PKK95_14540"
     CDS             complement(3677..>4277)
                     /gene="mreB"
                     /locus_tag="PKK95_14540"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HOC19488.1"
                     /translation="TAIGAGMPITEPAGNMIVDIGGGTTDIAVISLAGIVYSKAVRVA
                     GNEMDEAIIQYIKKTYNLLIGERTAEAIKMEIGSAFPLEERLNMEIKGRHLIEGVPKT
                     ITITDEEIREALAETVNVIVDAVRVALERTPPELSADIVDRGIVLTGGGSLLKNLDKR
                     LREETGLPLAMAEDPLSSVVLGAGKMLSDFNLLRKISID"
BASE COUNT          634 a         1555 c         1463 g          625 t
ORIGIN      
        1 gccggctctc gtcgaagaac ttggcgaggg ccagggccag cgcgaccttg gcgaactccg
       61 acggctgcag gttgaagaac ggcaggtcaa tccaccgccg cgcgccgccg ccggtcgtcc
      121 cgaacagcag cacgccgacc agcagcgcga cgatcaggcc gtagatggcg agcgagttgt
      181 cggccagcgt ccggtagtcg atcgtcaggc agaccatgaa gacgaacgcg ccgaggccga
      241 tggcgtagag ctggcgcacg tacagtcccg cgttcgcccc cgcgtgcgtg gtgctgtaga
      301 tcatcgccac gcccatcgcc gacagcgcgg cgatcgccgc aatcagcagc cagtcgatgt
      361 ggtaatagag gcggcgttcg agcatggcga tcccgcaggg ctgaagccct gcgctactgg
      421 ccggcggtcg cgattgtcgc caccgccggc gccggcgcgg ctggcggcgt gaaggtgggc
      481 agcggccgcc cctccttctt cgcgaaatag gtctccatca cgtacttcgc gatcggggcg
      541 gccaggtagc cgtgctcgga gtgctcggcg aagaccgccc ccgcgatctc ggggttgtcg
      601 cgcggcgcga agaagacgaa ccagccgtgg tcgcgcaggt ccatctcggt gcggccggcc
      661 gcggccctcc cgccctgcag cgagatgacc tgcgcggtgc ccgtcttgcc ggacacgtcg
      721 cgcccctgga tccgcgcccg cccgccggtg cccgcgccgt tgacgaccat ccacagcccg
      781 tcgtgcaggg cggccaccgt ctcgggcttg aaggcgaccc tcgacttcgg ctctggcgcc
      841 ggcaccggct tccagccgcc gccggcgtcc tcggcgcgca ccaggtgggg cgtcacgcgg
      901 gtgccgccgt tggccaccgt cgacatcatc acggccagct ggatcggcgt caccgacacg
      961 taaccctgcc cgatggcgac cgagatcgtc tcgcccgagt accacttctc gccgaagcgc
     1021 gcctgcttcc aggcggtgga cggcatgatc ccgtcgatct cgttcggcag gtcgatgccg
     1081 ctcttctcgc cgagccccag cagggtcgcc cacttgtgca gccggtcaat ccccaccatg
     1141 ttgcccagcg tgtagaagta cacgttgcac gaccgctcga tcgcgtgccg catgtccacg
     1201 ctgccgtggc ccctcttctc ccagcactgg aagtagcggc cgtagaaggt cgcccccccg
     1261 ccgcagtgca cgcggaaatc gggcgtcacc agcccctcct cgagggcggc cgtcgcgacg
     1321 gcgatcttga aggtcgatcc cggcgagtac cgtccctgga tggcgcggtt ctgcagcggc
     1381 cgcagcttgt cggtgttcag ctggttccag gtgacccggt cgatcccccc ggcgaaggcg
     1441 ttcgggtcgt acgccggcag cgacacgtag gcgagcacct cgcccgagcg cgggtcgagc
     1501 acgacggcgg cccccgcgta gccgtagccg cggaacccct cctcggccgc gcgctgcagg
     1561 tcgtagtcga gggtcagctg cacccgtcgc ccctctgacg gcgggatctc ctcgagcgtc
     1621 cggatctcgc ggccggtgct gttgacgacc acgcgccggg cgccgtcctg tcccatcagc
     1681 aggtggttgt agaccctctc gatccccgac tgcccgacga tgtcgcccgg ctgcagcccg
     1741 ccggcctgca cctgctcctc ggttgcctgc cccacgtagc cgaacaggtg cgcggccagc
     1801 gactccgagg gatagcgccg tgtcgggacc cgctccacga tgacgcccgg cagctcggtg
     1861 tcgaggcggc gcgccatcac ggccgccacc tgcgcgagcg aggcgtcctc gaccaccggg
     1921 atcgggcggt aggcgggcag gttgcggttg cgccgcaccg tctcgcgcac cgccgcctcg
     1981 tcgagctgcg cgacacggca gagcagccgg atggtctcgt cggcgtcctt gatgtgctcg
     2041 cgcaccagcg agatcacgaa cgagtcgcgg ttctccacca gcaccgctcc gtggcggtcg
     2101 tacagcacgc cgcggggggc gcgcagcgac agcgtccgct ggtggttgtt ctcggccagc
     2161 tcgcggaact tcgcgtgctg cacgacctgg aagtaccaga aggcgagcgc cagcacggcg
     2221 aacacgaccg tgatggccgc ctggatcccg ttcaggcgga ccgtgatctt ccggcggtcc
     2281 tgcggccgcg acaccgacgg ctcggcgggg gccagcagcc tcaccgcacc ctcccccgcg
     2341 acatcccgcg acggcggtcg atcatgccgg gaagcgcatc caggacctgg aagaacacca
     2401 cgccgagcag ggcgttggcc agcgattcgc cgagggcggc gtacagagtg acgaccgggg
     2461 gctgcgggtc gagcaggtag tagaggccgg tcgtgaggcc cacctgcacc agcgtcgccc
     2521 cgaagaacac gaggaaccgg ggaatggtct gcgacacgat gaaggtggtg ccgatgcggc
     2581 ccgccaggta gccgacgacg gtcttggcca ggccgccgac gccgacgatg ccgctgccga
     2641 gcgaatcctg cgccagcccg gcaatcgtcc ccgagaggat gccggcggtc gggcccgacg
     2701 tgaggccgag gtacacgacc gccacgagga cgaggtccac gccgccggct ccccgcacca
     2761 ggaaccgcgc gagcgtcgtc tggaggacga gcgcggcggc gatggtcacc agcacgcgca
     2821 gcgccgtcac cgcgacaccc cccgcgcctc ggacacgccg gtctgcccgc ttggcgccac
     2881 gacgacgagc accgcctcca ggctcccgaa gtcgacgctc ggcgcgacgc ggatcgactt
     2941 gaacaccccg gcggcccgct cgatccgctc gacccgcccg accaggaagc cgggggggta
     3001 gatgccgtcg ataccggagg tcgtcaggac gtcgccttct gtgatgtcgg ccgtggtggg
     3061 caggtactcg agccgaaggg tggtgtcgcc ggtgccgaac gcgacgccct gcgcacgcga
     3121 ccgttcgacg acgaccgcga ccgccgccga acggtcgatc agcagctgca ccttcgccgc
     3181 gtgccgggtc ggcatgacga cgcggccgac ggcgcccccc ggcgacacga cggccatgtc
     3241 ggtgagcacc ccgtggtcgg tgccgcggtc gatcgtgacc gttttgaagt cggtggtggc
     3301 gctggtgccg atgatctcgg cggcgcgggt gggcaggctg gtgcggtctc gcaggtcgag
     3361 cagctgacgc atgcgggtcg tgcggccggc ctcggcccgc gcgagctgga gctggacccg
     3421 cagctcggcc acctcgtggc gcaaccgctc gttctcggcc tgcgcgtcgc cgagccggac
     3481 gtagccctcc caaccccggt gcgtggcgtc catcacgccc gaggttgcgc gctgcacctc
     3541 ggccagcgcc ccgaacagca ccgcctgcag caccggcaca cccgaccggg tgttgacctg
     3601 cgcggaaatc aggatgatgt gcccgacggc ggcagccacg aacaggtagc cgctccgccc
     3661 gcgaatgccg gagaaattag tcaatcgata tcttccggag caagttgaag tccgaaagca
     3721 tcttcccggc ccccagcacg accgacgaca gcgggtcttc ggccatggcc agcgggagcc
     3781 ccgtctcctc gcggaggcgc ttgtccaggt tcttgagcag cgacccgccg ccggtcagca
     3841 cgatgccccg gtcgacgatg tcggccgaca gctcgggggg cgtgcgctcg agggccacgc
     3901 gcacggcgtc cacgatcacg ttgaccgtct cggcaagcgc ctcccggatc tcctcgtcgg
     3961 tgatcgtgat ggtcttcggc accccctcga tcaggtgccg ccccttgatc tccatgttca
     4021 accgctcctc gagcgggaac gccgagccga tctccatctt gatggcctcg gccgtgcgct
     4081 cgccgatgag gaggttgtac gtcttcttga tgtactggat gatcgcctcg tccatctcgt
     4141 tgccggccac gcgcaccgcc ttgctgtaga cgatgcccgc cagcgagatg acggcgatgt
     4201 cggtggtgcc gccgccgatg tccacgatca tgttgcccgc cggctcggtg atcggcatcc
     4261 cggcgccgat ggcggtc
//
LOCUS       DAOPQX010000337         4222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22781_length_4222_cov_3.797216, whole genome shotgun sequence.
ACCESSION   DAOPQX010000337 DAOPQX010000000
VERSION     DAOPQX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22781_length_4222_cov_3.797216"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKK95_14545"
     CDS             <1..777
                     /locus_tag="PKK95_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC19489.1"
                     /translation="EKGSFTGATEKQIGKFEQADGGTIFLDEVGDMSLKTQAKVLRVL
                     QEGEVERVGSSRTIKVDVRVIAATNKDLEREIEKGTFREDLFFRLSVIPIHVPPLRDR
                     PEDIPLLVRHFASLFARENNFRPRQITPGAMAALQRHRWKGNIRELRNTVERMIIMAP
                     REIIDVADLPEAVRVDVRPAAPDNTSGSRSLDTSRPPDTSRPPETLREFKELSERSFL
                     VQKLRENNWNISRTAEVIGTPRSNLYKKLEFYKIAQEIDG"
     gene            853..1447
                     /gene="rnpB"
                     /locus_tag="PKK95_14550"
     ncRNA           853..1447
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKK95_14550"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1534..2487
                     /locus_tag="PKK95_14555"
     CDS             1534..2487
                     /locus_tag="PKK95_14555"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC19490.1"
                     /translation="MSHYLVTGGAGFIGSHLAEELIRRGEHVRVVDNLSTGKLANLSH
                     LPTIEFVEGDLADLEVARRAVKGVDYVLHQAAIPSVPRSVSDPITSNRANVDATLNVL
                     VAARDEGVRRVVYAGSSSAYGNAPALPKHEEMPAAPLSPYALQKLVGEQYMAMFTRLY
                     GLETVTTRYFNVFGPRQDPSSPYSGVISLFISALVDGRQPTIYGDGEQTRDFTYVANV
                     VDGVLRACHTPGIGGEMINVATGGRISLNRLLAVLAELVGRRAEANYEDERPGDVRDS
                     QAAIEKAQRLTGYAPTVSLEEGLARTLAWYRDQEGIGRAAL"
     gene            2790..3134
                     /locus_tag="PKK95_14560"
     CDS             2790..3134
                     /locus_tag="PKK95_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOC19491.1"
                     /translation="MSRPHRLAALMLAALLLVPAVSLAQAPGQAAPPAASKTAPAATP
                     VNLNTATVEQLDGLPGIGRAMATRILEYRQKAGGFKKVEELMNVRGIGEKNFLKLKPL
                     VTVGPVKTSGQQ"
     gene            3152..3730
                     /locus_tag="PKK95_14565"
     CDS             3152..3730
                     /locus_tag="PKK95_14565"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HOC19492.1"
                     /translation="MRAGYSLAELVVTLALAALMAGMATPTVFGARDAIRALGAADYL
                     ASLLDVARTEALRRHANVALRFEPEATGFRYTMVADGNGDGVRTTQVLSGVDPAIRAG
                     ERLDEQFPGVTFGIADGVGPIDTGEPFDDRDPIRFGRSRMVSFSPTGTCTPGTLYIVG
                     RGRRQLAVRVLGATGRVRVLEFHPALAEWQPR"
     gene            3715..4065
                     /locus_tag="PKK95_14570"
     CDS             3715..4065
                     /locus_tag="PKK95_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOC19493.1"
                     /translation="MAAAMIRDRRMARRWQADDCMWLSNARLRPGPDVLVCDLSAGGA
                     RVDAPIRLVPGGRVELVLSGPGWQWSVEASVVHVRLCALASEGPRYLAGLRFSRRLEI
                     ALLREARPKAAWAG"
BASE COUNT          645 a         1472 c         1486 g          619 t
ORIGIN      
        1 gagaagggct cgttcaccgg cgccaccgag aagcagatcg gcaagttcga gcaggccgac
       61 ggcgggacga tcttcctcga cgaggtcggc gacatgagcc tgaagacgca ggccaaggtg
      121 ctgcgcgtgc tccaggaggg ggaggtcgag cgcgtcgggt cgtcgcggac gatcaaggtg
      181 gacgtgcggg tgatcgccgc caccaacaag gacctcgaga gggagatcga gaagggaacc
      241 ttccgcgagg acctgttctt ccgcctgagc gtgatcccga tccacgtgcc gccgctgcgc
      301 gaccgccccg aggacatccc gctgctggtg cgtcacttcg ccagcctgtt cgcccgggag
      361 aacaacttcc ggccgcggca gatcacgccg ggcgccatgg ccgcgctgca gcgccaccgg
      421 tggaagggga acatccgcga gctgcgcaac acggtcgagc ggatgatcat catggcgccc
      481 cgcgagatca tcgacgtggc cgacctgccc gaggccgtgc gggttgacgt gcggccggcg
      541 gcgccagaca atacgagtgg gtcccgctcg ctcgacacgt cgcggccgcc cgacacgtcg
      601 cgcccgcccg agacgctgcg cgagttcaag gagctgtcgg agcgcagctt cctggtgcag
      661 aagctgcgcg agaacaactg gaacatctcg cgcacggccg aggtgatcgg gacgccgcgc
      721 agcaacctgt acaagaagct cgagttctac aagatcgcgc aggaaatcga cgggtagccc
      781 cggcctccgt gccggtggaa cagcgctctt tgacaatttg gccgcagaac agccagacga
      841 tcgccgcccg gcgaaggtcc ctgggcgtcc gcgcccgggg agacgccgcc ggggggagga
      901 aagtccgaac tccgcagggc agtgcgccgg gtaacgcccg gtcagggtga cctgaaggaa
      961 agtggcacag aaaacatacc gccagtcttc gccctcgacg cttcgcattg cgaggctgcg
     1021 gccggcaggc ccctgaaggg cttgcccgcc ggagcttgag cagtggaagg gtcgagggcg
     1081 aaggcgggta agggtgaaaa ggtgcggtaa gagcgcaccg cgccgtctcg ccgaagcaac
     1141 ggccgggatt cccggccgtt gcgaaggcgg accgtgggac cggtccggtt ccgcgcgcgg
     1201 caacgcgggt ggcaggcaaa accccgcacg gagcaagacc gaatagggag gcgtcaccag
     1261 gacggcccgt ccgacgcttt cgccaacgtc ccggttcgcc ggggccgcgg cggggcgaca
     1321 gcctccgggt tggtcgctgg atcccgccag caatggcggg gctagaggaa tgatcgtcgt
     1381 ccgccggcgc ccggcgtgag ccgggcgaag gcggaaacag gattcggctt acggctgctc
     1441 tgcggcctct tcgttgttcc ggtttggcgg tctccgtttt cccgtgtccg tcttgcggtt
     1501 tccgttttcc gctcgctgtt ccgcgtttct cctatgtccc attacctagt caccggcggc
     1561 gccggtttca tcgggtcgca cctggccgaa gagctcatcc gtcgcggcga gcacgtgcgc
     1621 gtcgtggaca acctgtcgac cgggaagctc gcgaacctct cgcacctgcc cacgatcgaa
     1681 ttcgtggaag gcgacctggc cgacctggag gtggcccgcc gagccgtgaa gggggtggac
     1741 tacgtcctgc accaggcggc cattccctcg gtgccccggt cggtcagcga cccgatcacg
     1801 tcgaaccgcg ccaacgtgga cgcgacgctg aacgtgctgg tggccgcgcg cgacgagggc
     1861 gttcgccgcg tggtgtacgc cgggtcttcg tctgcttacg gcaacgcccc cgcgctcccg
     1921 aagcacgagg agatgccggc cgcgccgctt tcgccgtacg cgctccagaa gctggtcggg
     1981 gagcagtaca tggccatgtt cacgcggctc tacggcctcg agacggtgac cacccggtac
     2041 ttcaacgtgt tcgggccccg gcaagacccc tcatcgccgt actcgggggt catctcgctt
     2101 ttcatcagcg ccctggtgga cggccggcag ccgacgatct acggcgacgg cgagcagacg
     2161 cgcgacttca cctacgtcgc caacgtggtg gacggcgtgc tgcgagcctg ccacacgccc
     2221 gggatcggcg gggagatgat caacgtggcc accgggggcc ggatctccct caaccggctg
     2281 ttggccgtgc tggccgagct ggtggggcgc cgggccgagg ccaactacga ggacgagcgc
     2341 ccgggcgacg tccgcgactc ccaggcggcc atcgagaagg cgcagcgcct gacgggctac
     2401 gcgccgaccg tatcgctcga ggagggcctg gcgcgcaccc tggcctggta ccgcgatcaa
     2461 gaggggatcg gccgggcggc cctctagctc gcaaccgcgg ttcaccgttt tctccccctg
     2521 agcggctggc cggtaacgcc ggtagccgcc tcgacgcgcg cctggaagcc ggccccacgc
     2581 tccgggcccg cgccggcccc tatctcccac ggtttcaagc cgttcgaacg acggccgatc
     2641 gccgtccatc acggtccgcc gcttgccgcg ccttcgggcg gtcccggcag gagcccgcgc
     2701 gggcgtccag acctccgtca gcgtggcgtt gccgcgccgg ggcctttcac cccgggccgt
     2761 taccggcccg cactcactgg aggagcgaca tgtcacgacc gcaccgcctc gccgccctga
     2821 tgctcgccgc cctgttgctg gtgcctgccg tctcgctggc acaggcgccg gggcaggcag
     2881 cgccgcctgc cgcctcgaag accgcccccg cggccacccc ggtgaacctc aacaccgcca
     2941 cggtcgaaca gctcgacggc ctgcccggca tcggccgtgc gatggccacc cgcatcctcg
     3001 agtaccgcca gaaggccggc gggttcaaga aggtcgaaga gctgatgaac gtccgtggca
     3061 tcggcgagaa gaacttcctg aaactgaagc cgctcgtcac ggtcggtccc gtgaagacca
     3121 gcggccagca gtagcgccgg acggaggcag gatgcgcgcc gggtactcgc ttgccgagct
     3181 cgtggtcacc ctcgcgttgg cggcgctgat ggcggggatg gcgactccca ccgtcttcgg
     3241 cgcccgcgac gcgatccggg cgctgggcgc ggccgactac ctggcgtccc tcctggacgt
     3301 ggcgcgtacc gaggccctca ggcgccacgc caacgtcgcg ctccggttcg agcccgaggc
     3361 gaccggcttc cggtacacca tggtcgccga cggcaacggc gacggcgtcc gcaccaccca
     3421 ggtactgagc ggggtcgatc cagctatccg cgccggggag cggctcgacg agcagttccc
     3481 cggcgtcacc ttcggcatcg ccgacggggt ggggccaatc gacacggggg agccgttcga
     3541 cgaccgcgac ccaatccgct tcggccgcag ccgaatggtc tcgttcagtc ccacgggcac
     3601 ctgcacccct ggaacgctgt acatcgtcgg gcgagggaga cgccagctgg ccgtgcgcgt
     3661 gctgggtgcc accgggcgcg tgcgcgtgct ggagttccat cccgccctcg ccgagtggca
     3721 gccgcgatga ttcgcgaccg gcggatggcc cggcgctggc aggccgacga ctgcatgtgg
     3781 ctgtcgaacg cccgcctgcg gccggggccc gacgtcctgg tctgcgacct gtcggccggg
     3841 ggagcgcgcg tcgatgcccc aatccgcctc gttccgggcg gacgcgtcga gctcgtcctc
     3901 tcgggcccgg gctggcagtg gtccgtcgaa gcgtcggtcg tgcacgtccg attgtgcgcc
     3961 cttgccagcg aagggccccg ctacctggcg ggtctgcgct tttcacggcg cctggagatt
     4021 gcgctgctgc gcgaggcgcg acccaaggcc gcctgggcag ggtagccgct acccgcgagg
     4081 ggaggggcta cctcccatgg gaacaggcta cccgtcgcgg gttgcctgga gcccgccggc
     4141 ccgcggtgac ttcgtcgtat ccggagtccg aagctctggc acatccattg aatcgggcgc
     4201 ctgggggcat tcagcaggag gc
//
LOCUS       DAOPQX010000338         4210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22872_length_4210_cov_2.998315, whole genome shotgun sequence.
ACCESSION   DAOPQX010000338 DAOPQX010000000
VERSION     DAOPQX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22872_length_4210_cov_2.998315"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..752
                     /locus_tag="PKK95_14575"
     CDS             444..752
                     /locus_tag="PKK95_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOC19494.1"
                     /translation="MGRKLYVGNLPYDVGEADLEQLFSAAGTVDSVNVMRDMATGRAR
                     GFAFVEMATDDEAQKAISQFNAYSLGGRSLTVNEARPKPERSGFGGGGGSRRRSEPRW
                     "
     gene            848..1030
                     /locus_tag="PKK95_14580"
     CDS             848..1030
                     /locus_tag="PKK95_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19495.1"
                     /translation="MKSSPSFLKRQKEKARQERQQQKSARRAEANARKATKPRAADGE
                     DPDIAGIVPGPQPPQE"
     gene            1072..2688
                     /locus_tag="PKK95_14585"
     CDS             1072..2688
                     /locus_tag="PKK95_14585"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC19496.1"
                     /translation="MGGCHAIAVPLLLLALAPGASAQIRPTVAPAPAAAGLRAAPPRF
                     TDTARREKLARAFPEIDRLVAAHVKREHIPGAAWAIVVDGALAHVGASGTRRAGVEAP
                     VDADTVFRIASMTKSFTAMAILVLRDEGRLSLDDPVERHVPELRGLHYPTADSPRITI
                     RHLLSHSAGFPEDNPWGDQQLAATEGQFTEMLRRGFPFSNPPGLAYEYSNLGFAILGR
                     VVARVSGMPYREFVASRILEPLGMTSTRLEAAEVPAGRLANGYRWEDGKWREEPPLPD
                     GAFGAMGGMLTSVRDLAQYVGAFLSAWPARDDPDAGPVRRASLREMQQVSRSQQATVS
                     LGPGGQVRLDAGGYGYGLRVGQTCEFGHLVWHSGGLPGFGSLMRWLPEYGVGFIAFGS
                     RTYAGWGGVAEQAFGLLAATGGLQPRAVQPSPALLAAKDTVSRLFASWDDQLIEAAAA
                     VNLFLDRSKERRRAEFAALQDRLGACRAHESFERIENALRGDWVLQCDRGRARASVTL
                     APTLPPTIQYMDVREAPAKESASRRPSCPR"
     gene            2729..4039
                     /gene="pfp"
                     /locus_tag="PKK95_14590"
     CDS             2729..4039
                     /gene="pfp"
                     /locus_tag="PKK95_14590"
                     /inference="COORDINATES: protein motif:HMM:NF041103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOC19497.1"
                     /translation="MSAPKKLAILVGGGPAPGINSVISAATIRAQLEGVEVVGIRDGF
                     ESVMQGNVDNISALTIDQVSRIHFRGGSHIGISRANPTKNPQHLENTVISLLRLNVAQ
                     LLTIGGDDTAYSAMKLEEKAGGRIHVAHVPKTIDNDLDLPPYVDTFGFQTARHIGVDI
                     VKNLMVDARTTSRWYFVIAMGRKAGHLALGIGKAAGATLTLIPEEFQGRRIHLNEIVD
                     TLAGAIIKRLSYGRRDGVAVIAEGLVLDIAPEDLAQLGDVEKDAHGNVRIAEVNIGEI
                     LKAEVTRRLKGFGIKATLVAKNIGYELRCADPIPMDMEYTRDLGYCAAKFLLSGGNAA
                     MISMQGGQFVPIPFTDLLDAATGRAKIRMVDVNSTRYAIARRYMIRLRRDDFDDPHEL
                     AKFAATAGMSLQQFRAEFESLIANEPPPYQIDIHPKKGEPGGHE"
BASE COUNT          680 a         1499 c         1443 g          588 t
ORIGIN      
        1 gccggctgag gaaggcgagc gggcaccgcg cgtggcggcc gccccgctcg ccgcggtcgc
       61 cctcgcggtg gccgtcgcgg cgatcctctg gctcggcgtc tacccgtccg ccgtcctgga
      121 cgcggtccgg gaggcctcgg cctcacttgc cggcctgagc tcctgagaac gaccgggctc
      181 tcgagttgga ccggaacccg cgcgaaatgt ggcggcgtgt gttacactga cggggtcagg
      241 ttggacaacg catttccgct tcgccgcgtc tgctcgaatc gagcctgccg tctcgcttcc
      301 ccgcacacgg cctctggaag agcttttcct ccatcgcact cgaggtccac gtccgggccc
      361 ggtcgatcgt acacccgatg aacggacgct gacggtccgc catcaaaacc tcccgccgca
      421 ggcggggagg agacagaaac gacatgggta gaaagctcta cgttggcaac ctcccgtacg
      481 acgttggcga ggccgatctc gagcagctgt tctcggctgc cggcaccgtc gacagcgtga
      541 acgtcatgcg ggacatggcg accggccggg cccgcggatt cgcgttcgtc gagatggcga
      601 ccgacgacga ggcgcagaag gcgatcagcc agttcaacgc ctactcgctc ggcggccgca
      661 gcctcaccgt gaacgaggcg cgccccaagc ccgagcgctc gggattcggg gggggcggcg
      721 gcagccgtcg ccggtcggag cctcgctggt agtccactga agcaggggca gggccctgct
      781 tcgccggcag cgtggagcgg ggcctcgacc ctgctccacg tcacctttca cccaggaggt
      841 cacccgcatg aagagcagcc cgtcattcct caagcgacag aaggagaaag ctcgacagga
      901 acgccagcag cagaagtcag cccgccgcgc cgaggcaaac gcccgcaagg cgaccaagcc
      961 acgtgccgcc gacggcgagg accccgacat cgccggcatc gtccccggac cgcaaccccc
     1021 gcaggagtaa cgtcccgttc ccgggcgcgc cggcccccga ggaggctcgt catgggtggt
     1081 tgccacgcga tcgccgtccc gcttcttctt ctcgcgctcg ccccgggagc aagtgcccag
     1141 attcggccga cggttgcgcc ggcacccgcc gcggcagggc tcagggccgc ccccccgcgg
     1201 ttcaccgaca ccgcccgccg ggaaaagctg gcgagggcct tccccgagat cgaccgcctg
     1261 gtggccgccc acgtgaagcg cgagcacatc cccggggcag cctgggcgat tgtcgtggac
     1321 ggcgcgctgg cgcacgtcgg cgcctcgggt acgcgccggg ctggcgttga ggcgcccgtc
     1381 gacgccgaca cggtcttccg catcgcgtcg atgaccaaga gcttcacggc gatggccatc
     1441 ctcgtgctgc gcgacgaagg ccggctgtcg ctcgacgacc cggtcgagcg ccacgtcccc
     1501 gagctccggg gcctccacta cccgaccgcc gactcacccc gcatcaccat ccgccacctg
     1561 ctctcccact cggctggctt ccccgaagac aacccgtggg gcgatcagca gctcgccgcg
     1621 accgagggcc agttcaccga gatgcttcgc cgcggctttc cgttctcgaa cccgccgggg
     1681 ctcgcctacg agtactcgaa cctggggttc gcgatcctgg gccgcgtggt ggcccgcgtg
     1741 tcgggcatgc cctaccgcga gttcgtggcg tcccggatcc tggagccgct cggcatgacc
     1801 tccacgaggc tcgaggccgc agaggttccg gccgggcggc tggcgaacgg ctaccggtgg
     1861 gaggacggga agtggaggga ggagccgccg ctgcccgacg gggcgtttgg agcgatgggc
     1921 ggcatgctga cctcggtccg cgacctcgcg cagtacgtcg gtgcctttct ctcggcctgg
     1981 cccgcccgcg acgatcccga tgccggcccg gtccgccggg cgtcgctcag ggagatgcag
     2041 caggtctcgc gctcgcaaca ggcgacggta tcgctgggcc ctggcgggca ggtgcgtctt
     2101 gacgccggag ggtacggcta cggcctgcgc gtggggcaga cctgcgagtt cggtcacctc
     2161 gtctggcaca gcggcgggct gcccggcttc ggatcgctga tgcgctggct gcccgagtac
     2221 ggcgtcgggt tcatcgcgtt cggcagccgg acgtacgccg ggtggggcgg cgtggcagag
     2281 caggcctttg gcctgctcgc cgcaacgggc gggcttcaac cccgggcggt ccagccgtcg
     2341 ccggcgctgc tcgcggccaa ggacacggtc tcgcgcctct tcgcgtcgtg ggacgaccag
     2401 ctgatagagg cggccgccgc agtcaacctg ttcctcgacc ggtcgaagga gcggcggcgg
     2461 gccgaattcg cggccctgca ggaccggctc ggcgcctgcc gggcgcacga gtcgttcgag
     2521 cggatcgaaa acgccctccg gggcgactgg gtgctgcagt gcgaccgggg aagggcccgg
     2581 gcatcggtga cactggcgcc aaccctgccg ccgacgatac agtacatgga cgtccgggag
     2641 gcgcccgcga aggagagcgc ctcgcgcagg ccctcgtgcc cccgctgagg aactcgccat
     2701 cattcaaccg aacgtggagg acaccaacgt gagcgccccg aagaaactcg cgatcctggt
     2761 cggcggcggc cccgccccag gtatcaactc cgtcatcagt gcggccacca tccgcgccca
     2821 gctcgaggga gtcgaggtcg tcggcatccg cgacggcttc gagtcggtca tgcagggcaa
     2881 cgtggacaac atcagcgccc tgacgatcga ccaggtgagc cgcatccact tccgaggggg
     2941 ctcgcacatc ggcatctcgc gcgccaaccc gaccaagaat ccgcagcacc tcgagaacac
     3001 cgtgatctcg ctgctgcgcc tcaacgtcgc gcagctgctg acgatcggcg gcgacgacac
     3061 ggcctactcg gcgatgaagc tcgaggaaaa ggcgggcggc cgcatccacg tcgcccacgt
     3121 gccgaagacc atcgacaacg acctcgacct gccgccctac gtcgacacgt tcgggttcca
     3181 gacggcgcgc cacatcggcg tggacatcgt caagaacctg atggtcgacg cccgcacgac
     3241 ctcgcggtgg tacttcgtca tcgcgatggg acgcaaggcc gggcacctcg ccctcggcat
     3301 cggcaaggcg gccggcgcca cgctgacgct catccccgaa gagttccagg ggcggcggat
     3361 ccacctgaac gagatcgtgg atacgctcgc cggcgccatc atcaagcgcc tgagctacgg
     3421 gcggcgcgac ggtgtggccg taatcgccga ggggctcgtc ctcgacatcg cgccggagga
     3481 cctggcccag ctgggcgacg tcgagaagga cgcccacggc aacgtgcgga tcgccgaggt
     3541 gaacatcggc gaaatcctca aggccgaggt gacccgccgg ttgaagggct tcgggatcaa
     3601 ggccacgctg gtcgccaaga acatcggcta cgagctgcgc tgcgccgacc cgatcccgat
     3661 ggacatggag tacacgcgcg acctcggata ctgcgcggcc aagttcctgc tctcgggcgg
     3721 caacgcggcg atgatctcca tgcagggcgg acagttcgtc cccatcccct tcaccgacct
     3781 gctcgatgcc gcgacggggc gggcgaagat ccggatggtc gacgtcaact cgacgcgcta
     3841 cgcgatcgcc aggcggtaca tgatccggct tcgcagggac gacttcgacg acccgcacga
     3901 gctggcgaag ttcgcggcaa ccgccgggat gtcgctccag cagttccggg ccgagttcga
     3961 gtcgctcatc gccaacgagc cgccccccta ccagatcgac atccacccga agaaggggga
     4021 gccagggggg cacgaatagc gcgggaacgc cagcgggaga cagtcgatga gccgggtgaa
     4081 ctactccacc agcagcccgt gggagccgat cgtcggctac tcgcgggccg tgcggtgcgg
     4141 cccccaggtg ttcgtcaccg gcaccacggc cacgggccct gaggggaagc tggttggcgt
     4201 cggcagcgcc
//
LOCUS       DAOPQX010000339         4194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_22961_length_4194_cov_3.885480, whole genome shotgun sequence.
ACCESSION   DAOPQX010000339 DAOPQX010000000
VERSION     DAOPQX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22961_length_4194_cov_3.885480"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(525..1049)
                     /locus_tag="PKK95_14595"
     CDS             complement(525..1049)
                     /locus_tag="PKK95_14595"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HOC19498.1"
                     /translation="MTYEAVPNTDIWHIPEEVVILRWRHRPASGGRTYDGFRLPPGTW
                     RTVLACPPSLDWMTASEQQVANALQEVRRKHDGRPLRSPGAANLLLLVRYLSRRGKLG
                     GILQRHRHVDPKSPGIPDVFLYWKNGDGAVEGGRFVEVKKPDERVRQCQIDEIRCLRE
                     AGLNAGVFRLIERW"
     gene            complement(1156..2223)
                     /locus_tag="PKK95_14600"
     CDS             complement(1156..2223)
                     /locus_tag="PKK95_14600"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HOC19499.1"
                     /translation="MRSGKAALSSVLAPRDRAAEHLLVPAGRRALTREALEARCLPRL
                     ARLLERTAIRHGEDHGRHWKPGDFDILLVETTVAPDAVLYVQFWSEPGQPTLWEVCSG
                     TPNPGARRFITARARRIIGEMGFAIGGQAGNFRKKVLVGNSGDALAAAGDVLRIFHEA
                     FRYRGATPLVARVVEAERSGRAVVHDRFTPEDLVNILRHRGCVTELKKGRQRQPLVFV
                     DAGGFRFVVALEVPADDGCFRCLDVTAMVGHVEGDTEAAWLTALNELNARSRVARAWV
                     DADGDVLVGTSVMCTRGVTEDYVVEQIEAWLQSAQDLLNNRPAAAGQQEADDVADGEG
                     AVDVAEGGRAPRRRRPTEVVH"
     gene            2434..3327
                     /locus_tag="PKK95_14605"
     CDS             2434..3327
                     /locus_tag="PKK95_14605"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOC19500.1"
                     /translation="MSEARVAAERCAALGASSQQFEQYAARQVKLGELPVLRALPVRN
                     RRLIGPWCFLDRFGPLTFAAGNPMDVAPHPHIGLQTVSWLLDGEVVHDDSLLQQALLR
                     PGGVNVMTSGAAIAHSERTPDDNTGRLNGVQLWAALPDAHRGGAPAFQHLPEVPAFEE
                     RGGIARVFSGTLGSARSPARHYSDLVGADLEVHAAGSLAVPLNPEHEHGVMLLGGDAT
                     LEGQALDPATLYYLGTRRSEVTMASGRGARVLLVGGRPFGETILMWWNFVRRFGDVPG
                     YPGARLAAPELAKLARPNPVS"
     gene            3350..3520
                     /locus_tag="PKK95_14610"
                     /pseudo
     CDS             3350..3520
                     /locus_tag="PKK95_14610"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
     gene            complement(3635..>4194)
                     /locus_tag="PKK95_14615"
     CDS             complement(3635..>4194)
                     /locus_tag="PKK95_14615"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOC19501.1"
                     /translation="ALAVNVAAERAAAVLETWYGGEEIGTAIAETLAGTNNPAGRLPV
                     TFYKSVNQLPPFEDYAMKGRTYRYFEGEALYPFGFGLSYSTFEYSALRAARGGEGATI
                     TARVRNTSAVDGDEVVQLYVKGGGNPGDPIRQLRGFERVRLRAGEEREVTFTMPVTDL
                     PKGKVHVSVGGGQPLGATPRVEGEV"
BASE COUNT          603 a         1519 c         1432 g          640 t
ORIGIN      
        1 cggagtcctg cgggtggccg cgcgatccgg cccgcagcgc ctcggtggcc gccgcgttct
       61 atctgtcgtt gccggcgacc gcccagctct ggaggtgtgc ggaggagttc gtcctcgctg
      121 atgtcgatgg cattcagcgc gcgctgggtg atggccacac ccagccagga gcactccgga
      181 caacgactca cggcgaccct aacaaccgac ctacccgccc tgagtcgtaa ctgctgcagg
      241 ggcttctttt cacgctgcct ttctctgtcc gccctgtcga tgatcttcgc gtcgtaccat
      301 gacagatcgc gtccaggctc gggtggctca ctcctctcgg cgccgcgtgc agcaccgcca
      361 gcctgcggaa cggcttgccg accgacatgc ggagatacat cccggccatc tgcgccacat
      421 cgtcccgggc cgtctccggg tccgcggtgt tggggtggga aggcgcggga gctctggcgc
      481 ctgccggaac gccgcgggtg gccttcacct tgtcgagcgg cctctcacca ccgttcgatg
      541 agccggaaca cccccgcgtt gagaccggcc tcacgtaggc aacggatctc gtcaatttgg
      601 cattgacgca cccgctcgtc gggcttcttc acctcgacga accgcccgcc ttcgacggcc
      661 ccgtcgccgt tcttccaata gaggaagacg tccgggatcc caggggactt cggatcgacg
      721 tgacgatggc gctggaggat gccgccaagc ttcccccgcc gcgacaggta ccgaaccaac
      781 agcaggaggt tcgcggcacc gggagaacgc agcggccggc catcgtgctt ccgccgaacc
      841 tcctgcaggg cattcgcgac ctgctgctca ctggcggtca tccagtcgag cgacggcgga
      901 caggcgagga ccgtgcgcca ggtgcccggt ggcaggcgaa agccatcgta cgtgcggccg
      961 cccgaagccg gccggtgccg ccaacggagg atgaccacct cctcggggat gtgccagatg
     1021 tcggtgttgg gcacagcctc gtaggtcacg cggtgcaaac gcttgcgcta ccgatgcaac
     1081 ccgtggccgg cggaacagac taaccgccgg gcatcgaaga cacaagctca acgggagatg
     1141 cgcgcatcgc cggcgtcagt gcacgacctc ggtcggacgc ctgcggcgcg gcgcacggcc
     1201 gccttcagcc acgtccacgg ccccttcccc gtccgcaaca tcgtctgcct cctgctggcc
     1261 agcggccgcg ggccggttgt tcagcaggtc ctgcgcggac tgcagccagg cctcgatctg
     1321 ctccaccacg tagtcctcgg tcaccccgcg cgtgcacatc acgctcgtgc caaccagcac
     1381 gtcgccgtcc gcgtccaccc acgccctcgc cacccgcgaa cgggcgttca gctcgttcag
     1441 cgccgtgagc cacgccgcct cggtgtcgcc ctcgacgtgc ccgaccatcg ccgtcacgtc
     1501 caggcaccgg aagcacccgt cgtccgcagg gacctccagc gccacgacaa agcggaagcc
     1561 gccggcgtcg acgaacacga gcggctggcg ctggcgcccc ttcttcagct cggtcacgca
     1621 gcccctgtgg cggaggatgt tgaccaggtc ctcgggggtg aagcggtcgt ggacgacggc
     1681 gcggccggag cgctcggcct cgacgacccg cgcaaccagg ggcgtcgccc cgcgataccg
     1741 gaacgcctcg tggaagatgc gcagcacgtc ccccgcggcc gcgagcgcgt cgccggagtt
     1801 gccgaccagg accttcttgc ggaagttgcc ggcctggccg ccaatcgcga agcccatctc
     1861 gccgatgatg cgccgggccc gggcggtgat gaaccggcgc gcgccgggat tgggagtccc
     1921 cgagcagacc tcccagaggg tcggctgacc gggctcggac cagaactgca cgtagagcac
     1981 ggcgtcgggc gcgacggtgg tctcgacgag caggatgtcg aagtcgcccg gcttccagtg
     2041 ccggccatgg tcctcgccgt gccggatcgc ggtccgttcg agcagccggg caagacgcgg
     2101 caggcagcgg gcctcgagcg cctcccgggt gagcgcgcgc cggccggcag gcaccagcag
     2161 gtgctcggcg gcgcggtctc gcggcgcaag cacggacgac aaggcggcct tcccggatcg
     2221 catccgcgtt tcctcccttc gctcccagcc gccgctgaaa tgcggcggtc agcgaccgga
     2281 gcagtgtcgc atgcgggcgt gacaggagag gtcaggacgc ctggcagcgc tgctcatggc
     2341 cgggcagtgc ggttcggtgg ggaaccgcgc cgggccggtc gggacgtcgt acaatgccga
     2401 cgggtttccc gcaggccgcg agaggaggat tcgatgagcg aagctcgcgt cgccgcggag
     2461 cggtgcgccg cgctcggcgc cagcagccag cagttcgagc agtacgccgc gcggcaggtg
     2521 aagctcggcg agctgcccgt cctgcgcgcg ctgcccgtgc ggaatcgccg cctcatcggc
     2581 ccgtggtgct tcctggaccg gttcgggccg ctcaccttcg cggccgggaa cccgatggac
     2641 gtcgccccgc accctcacat cggtctccag acggtgagct ggctgctcga cggcgaggtg
     2701 gtgcacgacg acagccttct gcagcaggcc ctcctgcggc cgggcggggt gaacgtgatg
     2761 acctcgggag cagcgattgc ccactccgag cgcacgccgg acgacaacac gggccggctg
     2821 aacggcgtgc agctgtgggc ggcgctcccg gacgcgcacc ggggcggggc gcctgccttc
     2881 caacacctgc ccgaggtccc ggccttcgag gagcgcggcg gcatcgcgcg cgtcttctcg
     2941 gggacgctcg gctccgccag gtcgcctgca cggcactact cggacctggt cggcgcggac
     3001 ctcgaggtgc acgccgcggg ctcgctcgcc gtccccctga atccggagca cgaacacggg
     3061 gtgatgctgc tcggcggcga cgcgacgctc gaagggcagg cgctcgaccc cgccacgctg
     3121 tactacctcg gaacccggcg ttcggaggtg acgatggcga gcgggcgcgg cgcgcgggtg
     3181 ctgctggtcg gcggccggcc tttcggcgag acgatcctca tgtggtggaa cttcgtccgc
     3241 cggtttggcg acgtgccggg ctaccccggt gcgcgccttg ccgcgcccga actggcgaag
     3301 ctggccaggc cgaacccggt cagttgaacc ttgacccctc ccctggacga tgccatcgtc
     3361 gtcccgcttc tacttcttct cccgggagga gacgcgccct cacgtgcatg tggagcacgt
     3421 agccggtgag gcgaagctat ggttggagcc ggctgtcagc ctcgcgtaca actacgggtt
     3481 gtcggccagg cgcatcggtc gtgttctggc cctagccgag gagcaccaca cgcggagggg
     3541 ccgtaccgaa ggtgtgactt ttctcacgct ttcggtacgg ccctacggcg tgcgattttt
     3601 cacgcgtccg gtacggcccc cggtgtgctc ggcgctagac ctccccttcc acccgcggcg
     3661 tggcgccaag cggctggcct ccgcccacac tgacgtggac cttgcccttc ggcaggtcgg
     3721 tcaccggcat cgtgaacgtc acttcgcgtt cttcgccggc gcggaggcgc acgcgctcaa
     3781 agccgcgcag ctgacggatc ggatcgccgg gatttccccc gcccttcacg tacagctgca
     3841 ccacctcgtc gccgtccacg gccgacgtgt tgcgcacgcg cgcggtgatc gtcgcaccct
     3901 cgccgccccg cgccgcccgc agcgccgagt actcgaaggt cgagtagctc aggccgaagc
     3961 cgaacggata gagcgcctcg ccctcgaagt agcggtacgt gcgccccttc atggcgtagt
     4021 cctcgaaggg cggcagctgg ttcacgctct tgtagaacgt gacgggcagg cgcccggccg
     4081 ggttgttcgt gccggcgagc gtttccgcga tcgccgtgcc gatctcctcg ccgccgtacc
     4141 acgtctcgag gacggccgcg gcccgctcgg ccgcgacgtt caccgcgagg gcgc
//
LOCUS       DAOPQX010000340         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_23068_length_4180_cov_3.164606, whole genome shotgun sequence.
ACCESSION   DAOPQX010000340 DAOPQX010000000
VERSION     DAOPQX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23068_length_4180_cov_3.164606"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PKK95_14620"
     CDS             complement(<1..707)
                     /locus_tag="PKK95_14620"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF017903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOC19502.1"
                     /translation="MTPHSETDAMYQPARERYAALGVDAEAALAKLSSFSISIHCWQG
                     DDVGGFENAADAPGGGLAVTGNYPGKARTPGELRADFEKALSLIPGRHRMSLHASYAE
                     TCGRTVGRDELGVEQFQGWIAWAKEVGIGVDFNQTYFGHPRVDRGMTLTHPDEGIRRF
                     WIEHGIRCREIGAAFGRELGTTCVTNLWIPDGMKDTPADRRAPRERLTEALDAIFAVP
                     IDKRHNLDSVEPKLFGIG"
     gene            802..1764
                     /locus_tag="PKK95_14625"
     CDS             802..1764
                     /locus_tag="PKK95_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19503.1"
                     /translation="MRVTLLACAAGLVALGAAPAAQDLDGFMARVLERRDEAWRRLHD
                     YVLSAREQFQILGPGDVPLHGERREYAWYVREGFLIRSPVRANGVELKDAERREYEAK
                     WLEQERERERRAIEREAQRAEKRAEAGETGEGDPSLDEFVGSRGEPRFISEAYFLRFK
                     FEPGNYYLVGREQLEGRAVLRVEYYPSRLFGDEGEEEDREQPGRRRKDDRDEGDIDRK
                     MNKASLVTLWIDPAEHQIVRYTFDNMDFGFLPGRWLVRVDGASASMTMARVLDGVWLP
                     SRISMSAGLSLANGGYRFEYGREFFDYRKAEVGARIRSYVPRER"
     gene            1745..3067
                     /locus_tag="PKK95_14630"
     CDS             1745..3067
                     /locus_tag="PKK95_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOC19504.1"
                     /translation="MFPGNGSLGLLGAVALLVAGLAVPAPAAQPAVETIAEVRVHGNH
                     TTPDADVLALAGLTIGQPITPTTLAEAERRLRESGRFASVEIRKRYRSLDRLDQVALV
                     IVVREHPVVEDPASPTPAVLKPLRRLAASGMLLPILSYADGYGFTYGARVSFVHLVGR
                     DGRVSVPLTWGATRRAALEIDKRVPAGPIDRLAGGVETWRRENPFYERHERRSGGWLG
                     ASGRIAGPLRAGVRGSIANVSFNAAGAGSDAGATVAGLEERLSTYGVELSVDTRHDPV
                     FPRNALYASAVLEALRPETSGHANRRNAQARAYLGLVRQAVLSVRWQYSSSDAPLPPY
                     ERALLGGAASVRGYEAGHESGDNLMAASAELRLPLNSPLKISRAGVTLFVDRGRVWNH
                     GTRASDAPWRTGGGVGLFFLASLFQLNADVAVRDGGGVRVHFSTGLQF"
     gene            complement(3306..4031)
                     /locus_tag="PKK95_14635"
     CDS             complement(3306..4031)
                     /locus_tag="PKK95_14635"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19505.1"
                     /translation="MTLRAPVLLAIAVASLGAVSCGDVSRLKARCLAGEVASCTELGD
                     HYALGQGVERDLGRAGEAYSRACDGGAFDVCNALGEMYRETGPIEGGAERAEELFVRA
                     CEGGSSAGCLNLGLAFAEREDFARALALYERSCSAGWAAGCHQLGHSYEIGEGVKADP
                     QRAIAWYGQACDGELVDGCVSAAMLLLTGTLVPADLPGAQRFFARAVALLDEGCQAGN
                     DRQCTERDRLKTRIAIAQAGKGP"
BASE COUNT          587 a         1471 c         1476 g          646 t
ORIGIN      
        1 ccgatgccga acagcttcgg ctcgaccgag tcgaggttgt ggcgcttgtc gatcggcacg
       61 gcgaagatgg cgtcgagcgc ttcggtcagt ctctcgcgcg gcgcgcgccg atccgccggg
      121 gtgtccttca tcccgtcggg gatccagagg ttcgtcacgc aggtcgtgcc gagctcccgg
      181 ccgaaggcgg cgccgatctc gcggcagcgg atgccgtgct cgatccagaa ccggcggatt
      241 ccttcgtccg ggtgagtgag cgtcatcccg cgatccaccc gggggtggcc gaagtaggtc
      301 tggttgaagt cgacgccgat gccgacctcc ttcgcccacg cgatccaccc ctggaactgc
      361 tcgacgccca gctcgtcccg gcccacggtc cggccgcacg tctcggcgta gctggcgtgg
      421 aggctcatcc ggtgccgtcc cgggatcaac gagagcgcct tctcgaagtc ggcgcgcagc
      481 tcgcccgggg tccgggcctt gcccgggtag ttccctgtca cggccaaacc gcctccgggc
      541 gcgtccgccg cgttctcgaa cccgccgaca tcgtcaccct gccagcagtg gatcgagatc
      601 gagaatgaag acagcttcgc gagcgccgcc tcggcgtcca ctcccagggc ggcgtagcgt
      661 tcgcgggcgg gttggtacat ggcatcggtc tcggaatgag gtgtcatagt ctcctgccgc
      721 gtgcattgtt ggagaacccg gggggaggcg tcaacgtatc tcttagagat cagggatcag
      781 cggggaaggc ggagggccga tgtgagggtc acgttgctcg cgtgcgcggc ggggctggtg
      841 gcgctggggg cggccccggc tgcgcaggac ctcgatggct tcatggcgcg cgttctcgag
      901 cggcgcgacg aggcgtggcg gaggcttcac gactacgtgc tgagcgcgcg cgagcagttc
      961 cagatccttg ggcccggcga cgtgccgttg cacggggagc ggcgggagta tgcctggtac
     1021 gtccgggagg gcttcttgat ccgcagcccc gtccgcgcca acggggtcga gctgaaggac
     1081 gccgagcgcc gcgagtacga ggcgaagtgg ctcgagcagg agcgcgaacg cgagcgccgc
     1141 gccatcgagc gtgaggcgca gagggccgag aagagggccg aggccggcga gacgggcgag
     1201 ggcgacccgt cgctcgacga gttcgtcggc agccgcggcg agccccgctt catctccgag
     1261 gcctacttcc tgcgcttcaa gttcgagccc ggcaactact atctcgtggg gcgcgagcag
     1321 ctggaggggc gcgcggtctt gcgcgtcgag tactacccct cgaggctctt cggcgacgag
     1381 ggcgaggagg aagaccgcga gcagccggga aggcgccgga aggacgaccg cgacgagggc
     1441 gacatcgacc gcaagatgaa caaggcctcg ctcgtcacgc tctggatcga tcccgccgag
     1501 caccagatcg tccgctacac gttcgacaac atggatttcg ggttccttcc cgggcggtgg
     1561 ctggtccggg tggacggcgc ctcggcctcg atgaccatgg cccgcgtgct cgacggcgtc
     1621 tggctgccgt cgcggatctc gatgtcggcc ggcctcagcc tggccaacgg cggctaccgg
     1681 ttcgagtacg gccgcgagtt cttcgactac cggaaggcgg aagtgggtgc gcgtattcgc
     1741 agctatgttc cccgggaacg gtagcctcgg gctcctcggc gcggttgcgc tcctggtggc
     1801 cggcctcgcc gtcccggcgc ccgccgcgca gccggctgtc gagacgatcg cggaggtgcg
     1861 cgtccacggc aaccacacga cgccggatgc cgacgtgctc gccctggccg gcctcacaat
     1921 cggccagccc atcacgccga cgaccctggc cgaggccgag cgccgcctgc gggagagcgg
     1981 ccgcttcgct tctgtcgaga tccggaagcg ctaccggtcg ctcgatcgcc tcgaccaggt
     2041 cgcgctggtc atcgtcgtcc gcgagcaccc cgtggtcgag gacccggcct cgccgacgcc
     2101 ggcggtgctg aagccgctgc gccgcctggc cgccagcggg atgctcctcc cgatcctctc
     2161 gtacgccgac ggctacggct tcacctatgg ggcccgcgtg tcgttcgtgc acctggtcgg
     2221 ccgcgacggc cgcgtctcgg tgccgctgac ctggggagcg acgcggcggg cggccctcga
     2281 gatcgacaag cgcgtgccgg ccggcccaat cgatcgcctg gcgggcggcg tcgaaacctg
     2341 gcgccgtgag aatccgttct acgagcggca cgagcggagg tcgggcgggt ggctgggcgc
     2401 gtccgggcgc atcgccgggc cgctgcgggc cggggtgcgg ggctcgattg ccaacgtcag
     2461 cttcaacgcg gccggcgccg gcagcgacgc cggcgctacc gtggccggcc tcgaggagcg
     2521 gttgtccacc tacggagtcg agctctcggt ggatacgcgg cacgacccgg tcttcccccg
     2581 caacgcgctc tacgcgagcg ccgtcctcga ggcgcttcgt cccgaaacca gcggccacgc
     2641 gaaccggcgc aacgcgcagg cgcgcgccta ccttggcctg gtgagacagg ccgtcctctc
     2701 cgtccgctgg cagtactcct ccagcgacgc cccgctgcct ccctacgagc gcgccttgct
     2761 cgggggcgcc gcctcggtgc gcgggtacga ggcagggcac gagagcggcg acaacctgat
     2821 ggccgcgtcg gccgagttgc gcctgccgct caactcgccg ctgaagatca gccgcgccgg
     2881 ggtgactctc ttcgtcgaca ggggcagggt gtggaatcac ggaaccaggg cgtccgacgc
     2941 gccgtggcgg accggcggcg gggtgggcct cttcttcctg gccagcctgt tccagctgaa
     3001 cgcagacgtt gccgtgcgcg acggcggcgg cgtccgcgtc cacttcagca cggggctgca
     3061 attctaatcc cgacgctcac atgtattgag caaggaggtg tgtgttcgcg ggtcctgccg
     3121 cggggctccc cccgctcggc ttcgcgcgaa aagcacttgt cacgcgtgtc gcgctccagg
     3181 cgagcggggg ccctccgccc cgcgtcaccc gctcacggcc ttgctcgacc caagtgagcg
     3241 tcgggatagt ttctacattc ttccttttgc attctgcatt tcaggccgcg tcttccagcg
     3301 gcctgctacg gccctttgcc ggcttgcgcg atcgcaattc gcgtcttcag gcggtcgcgc
     3361 tcggtgcact ggcgatcgtt gcccgcctgg cagccttcgt cgagcagcgc aacggcgcgg
     3421 gcgaagaacc gctgcgcccc gggcaggtcg gcgggcacga gcgtgccggt cagcaagagc
     3481 atggcggccg agacgcaccc gtccacgagc tcgccgtcgc acgcctgccc gtaccaggcg
     3541 atcgcccgct gcgggtcggc cttcacgccc tcgccgatct cgtaagagtg accgagctgg
     3601 tgacacccgg cggcccagcc cgcgctgcac gatcgctcgt acagggccag tgcacgggca
     3661 aagtcctcgc gttcggcgaa cgcgagcccg aggttcaggc accccgcaga gctgccgccc
     3721 tcgcaggccc gaacgaacag ttcctcggcc ctctcggccc cgccctcgat gggcccggtc
     3781 tcgcggtaca tctccccgag cgcgttgcag acgtcgaacg cgccgccgtc gcacgcccgc
     3841 gagtacgcct cgccggcccg cccgaggtcg cgctcgactc cctgcccgag cgcgtaatga
     3901 tcgccaagct cggtgcagct cgccacctca ccggccaggc atcgcgcctt caggcgcgac
     3961 acgtcgccgc aggaaacggc gccgagtgag gcgaccgcga tcgcgagcag caccggggcg
     4021 cgaagggtca tttcaactct ccatcgggtg gaggaattct actagaaccg gaacgcggcg
     4081 gttgaaccgc gggcgggcga ggggccacaa tgagcctttc ctcgagagga gcttgcatgc
     4141 cgctacgcgc catcctcgcc ctgctggccc tcgccgggtt
//
LOCUS       DAOPQX010000341         4166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_23169_length_4166_cov_4.616395, whole genome shotgun sequence.
ACCESSION   DAOPQX010000341 DAOPQX010000000
VERSION     DAOPQX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23169_length_4166_cov_4.616395"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKK95_14640"
     CDS             complement(<1..205)
                     /locus_tag="PKK95_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOC19506.1"
                     /translation="MSGSVLPVDLAAVLGRLNGDRELLAELAVLYLEDEPSLIAELEA
                     AVGRGDAEATRRAAHAIKGSVANF"
     gene            complement(202..843)
                     /locus_tag="PKK95_14645"
     CDS             complement(202..843)
                     /locus_tag="PKK95_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC19507.1"
                     /translation="MRILIADDDRVSLRVLQGVLQDWGYDVVAARDGTEAWGVLSAPD
                     RPSLAVLDWMMPGMDGPELCRRVRALGGGSPIYVILLTARGDPGDIVAGLGSGADDYV
                     VKPFDGAELRARLSVGERVLKLQGDLADRVRDLEEALANVKRLQGLLPICAYCKKVRD
                     DRNYWQQVEQYLSERLDARFSHSVCPTCYERHVRPEIEKLRSRRRAGEKGRGA"
     gene            926..1408
                     /locus_tag="PKK95_14650"
     CDS             926..1408
                     /locus_tag="PKK95_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC19508.1"
                     /translation="MHLVLVDDEPAILDILAAACHADGHVVTTFGSSLDALAFLQQNS
                     VDLLITDIVMPPPDGFRLVSAARKIHPRLDAILVTGYTQRYSLEDVLACGASDLLFKP
                     FRLQELRARVRLAEERRRVLAHHLRVLTHALEDPTNELLQREARELRAALDLKLARPV
                     "
     gene            1474..>4166
                     /locus_tag="PKK95_14655"
     CDS             1474..>4166
                     /locus_tag="PKK95_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOC19509.1"
                     /translation="MHRPRLVRVLAAAWFLLACAASVPAQSPSYARDPKQPIDAAYTA
                     KIKEYTTAPFFSSPLVDYLPASKAVPTPQAVIGDIAGAPGKLPYSADVHRYFRMLEKA
                     SPRVRVFSIGRSEEGREMIAAAVADAAVLARMDENRARLAKLADPRTIGLDDRQAEAL
                     VAASVPVYYITGALHAGETGAVTALMELAYRLAVDEAPYIRAIRSNVITLITPVVEVD
                     GRDRQVDVYNWHLAHPKDNWPPVVYWGKYVVHDNNRDAMGVTLALTKNVLDTFVDQKA
                     QLLHDLHESVPYLYDNTVGDGPYNAWVDPLLADEWQLLGWNNMSELTRFGMPGAFAHG
                     TFDTWTPGYLMFIAAMHNGTSRLYETFGNGGADTVERTLSPREYSRTWYRQNPPLPKA
                     VWSQRNNNNYQQTGLLTALSYYASNRQLFLRNFYLKAKRSVLKPKNEGPAAYVLPADE
                     ARLGAQAALLRVLQKQRCEISRATAPFTVEVPARKPRPRGGDEGQAAAAKPDVKEAPK
                     TEPRQFPAGSYLVRMDQPYSRIADALLDYQYWSPSDPQKNPYDDTGWTLGELFHVQVV
                     RVTDSKVLEAPMEKVAGEVRADGGIAGAGPVLLLDHDADPSLIAFRYRLKDVAFETAE
                     EPFEAAGRKFHRGSFIIKPADPSAAKAAAADLGLQLHAVATAPEVKTHPVKTARIAYV
                     HSWLSTQDEGWWRMEFDRFGIPYTYISTQTLAATPDLRARYDVIVFPPVGRSSRQVVS
                     GLPLWGNPLPWKKTELTPNIGVIDSTDDIRPGLGWAGLANLETFVREGGVLITTADTS
                     ELAVSYGLTHGVSAQRGQRLNAPGTIVRAKFVDLQSPIAYGYGENLSIYTSNGLVFGL
                     SHSVGGRSGRWGDGGSDRPTGRGTADDPDVPGVGPEVPDGL"
BASE COUNT          650 a         1529 c         1369 g          618 t
ORIGIN      
        1 caaagttcgc gacgctcccc ttgatcgcgt gagccgcccg gcgggtggct tccgcgtcgc
       61 ccctgccaac ggccgcctcg agctccgcaa tcagcgaggg ctcgtcttcc aggtacagaa
      121 ccgcgagttc ggccaacagc tcgcggtcgc cgttcaggcg gccgagcacg gccgccaggt
      181 caacaggcag tacggatccg ctcatgcgcc gcgccccttt tcgcccgccc gccgccgcga
      241 tcgaagcttc tcgatttctg gccggacgtg ccgctcgtaa caggtcggac agacgctgtg
      301 gctgaagcgg gcgtcgagcc gctcgctgag atactgctcg acctgctgcc agtagttcct
      361 gtcgtccctg accttcttgc agtacgcgca gatcggcagc agcccctgca gccgcttcac
      421 gttggcgagc gcctcctcga ggtcgcgcac gcggtcggcc aggtcgccct gcagcttcag
      481 cacgcgctct cccacgctca gccgcgctcg caactcggcg ccgtcgaacg gcttgaccac
      541 gtagtcgtcg gcccccgagc caagccccgc cacgatgtcg cccgggtctc cccgggcggt
      601 gagcaggatc acgtagatcg gagaaccccc gcccagtgcc cgcacccggc ggcacagctc
      661 agggccgtcc attcccggca tcatccagtc gagcacggcg agcgacgggc ggtcgggggc
      721 cgacaatacc ccccaggcct cggtgccgtc gcgggccgcc accacgtcgt agccccaatc
      781 ctgcagcacc ccctgcagca cccggagcga cacccgatcg tcgtctgcaa tcaggattct
      841 catggccgcc taaacttatg cctccgctcg tccgattaca agagtgatcc ccgaggccac
      901 gtttccggcg cggggcgaga ggcgcatgca cctggtcctc gtggacgacg agcccgcgat
      961 cctggacatc ctggcggccg cctgccacgc cgacggccac gtcgtcacca cgttcggttc
     1021 gtcgctcgac gcgctggcgt tcctgcagca gaactccgtc gacctgctga tcaccgacat
     1081 cgtgatgccg cccccggacg ggttccgcct ggtgagcgcc gcgcgcaaga tccacccccg
     1141 gctcgatgcc atcctggtca ccggctacac ccagcgctac tcgctcgagg acgtgctggc
     1201 ctgcggcgcc tccgatctgc tgttcaagcc gttccgcctc caggagctgc gggcgcgcgt
     1261 gcgccttgcc gaggagcgtc gccgcgtgct ggctcaccac ctgcgcgtgc tcacgcacgc
     1321 gctcgaggat cccaccaacg agctcctgca gcgcgaggcc cgcgagctgc gggcggccct
     1381 cgacctgaag ctggccaggc ccgtgtagga ggcccttcgg cctcccctcc cccgccttcg
     1441 tatacaatgc gcccgccccc gcgaggacct tccatgcatc gtccgcgact cgtccgcgtg
     1501 ttggcggccg cctggttcct cctggcgtgc gccgcgtcgg ttccggccca gtccccgtcg
     1561 tacgcccgcg atccgaagca gccgatcgac gccgcgtaca ccgcgaagat caaggagtac
     1621 acgacggcgc cgttcttctc gtcgcccctg gtggactacc tccccgcgtc gaaggccgtt
     1681 ccgacgccgc aggccgtcat cggcgacatt gccggcgcgc ccggcaagct gccgtacagc
     1741 gccgacgtgc accggtactt caggatgctc gagaaggcga gcccgcgggt gcgagtgttc
     1801 tcgatcggcc ggtccgaaga agggcgggag atgatcgcgg ccgccgtggc cgacgccgcg
     1861 gtgctggcca ggatggacga gaaccgggcg cgcctggcga agctggccga tccgcggacg
     1921 atcggtctcg acgatcggca ggccgaggcg ctcgtcgccg cctcggtgcc cgtctactac
     1981 atcaccggcg cgctccacgc gggcgagacc ggcgccgtga ccgccctgat ggaactggcg
     2041 taccggctgg cggtggacga ggcgccttac atccgtgcca tccgctcgaa cgtgatcacg
     2101 ctgatcacgc cggtcgtcga ggtggacggg cgcgatcgcc aggtggacgt ctacaactgg
     2161 cacctcgccc acccgaagga caactggccc ccggtcgtct actggggcaa gtacgtcgtt
     2221 cacgacaaca accgcgacgc catgggcgtg acgctggcgc tgacgaagaa cgtgctcgac
     2281 accttcgtcg accagaaggc gcagctgctg cacgacctgc acgagtcggt cccgtacctc
     2341 tacgacaaca cggtgggcga cgggccctac aacgcgtggg tcgatcccct gctcgccgac
     2401 gagtggcagc tgctcggctg gaacaacatg tcggagctga cccggttcgg gatgccgggc
     2461 gccttcgcgc acggcacctt cgacacctgg acgccggggt acctgatgtt catcgcggcg
     2521 atgcacaacg gcaccagccg cctgtacgag acgttcggca acgggggggc cgacacggtc
     2581 gagcggacgc tctcgccgcg cgagtattcg cgcacctggt accggcagaa cccgccgctg
     2641 ccgaaggcgg tctggtcgca gcggaacaac aacaactacc agcagaccgg cctgctgacg
     2701 gcgctgtcgt actacgcgtc gaaccggcag ctgttcctcc gcaacttcta cctgaaggcc
     2761 aagcggtcgg tgctcaagcc gaagaacgag gggccggcgg cctacgtcct gcccgccgac
     2821 gaagcccggc tgggggcgca ggcggccctt ctccgggtgc tgcagaaaca gcggtgcgag
     2881 atctcgcggg ccaccgcgcc gttcaccgtg gaagtgccgg cgcgcaagcc gcggccgcgc
     2941 ggcggcgacg aggggcaggc cgcggccgcg aagcctgacg tcaaggaggc gccgaagacc
     3001 gagccgcggc agttccccgc cggcagctac ctcgtccgga tggatcagcc ctacagccgc
     3061 atcgccgacg cgctgctcga ctatcagtac tggagcccca gcgaccccca gaagaacccg
     3121 tacgatgaca cgggctggac cttgggcgag ctgttccacg ttcaggtggt gcgcgtcacc
     3181 gactcgaagg tgctcgaggc cccgatggag aaggtcgccg gcgaggtgcg tgccgacggc
     3241 gggattgccg gtgcggggcc ggtgctcctc ctcgaccacg acgccgatcc gtcgctcatc
     3301 gcgttccgct accggctgaa ggacgtggcc ttcgagacgg ccgaagagcc cttcgaggcc
     3361 gccgggcgga agttccaccg cggcagcttc atcatcaagc cggccgaccc ctcggcggcg
     3421 aaggccgcgg ccgccgacct cggcctgcag ctgcacgccg ttgccactgc gcccgaggtg
     3481 aagacgcacc cggtgaagac cgcccgcatc gcgtacgttc acagctggct cagcacgcag
     3541 gacgaggggt ggtggcggat ggagttcgac cgcttcggca tcccctacac ctacatcagc
     3601 acccagacgc tcgccgccac gccggacctc cgggcgcggt acgacgtcat cgtctttccg
     3661 ccggttggac gaagctcgcg gcaggtggtg tcgggccttc ctctgtgggg caaccccctg
     3721 ccctggaaga agaccgagct gacccccaac atcggcgtca tcgattccac cgacgacatc
     3781 aggcccgggc tgggctgggc ggggctcgcc aatctcgaga ccttcgtgcg cgagggcggc
     3841 gtcctgatca ccaccgccga cacctctgag ctggccgtct cctacgggct cacgcacggc
     3901 gtgtccgcgc agcgcgggca gcgcctgaat gcgccaggca ccatcgtccg ggcgaagttc
     3961 gtcgatctgc agagtcctat tgcgtacggt tacggcgaga acctctcgat ctacacgtcg
     4021 aacgggctgg tgttcggcct cagccactcg gtgggggggc ggtcgggacg gtggggcgac
     4081 ggcggcagcg accggccgac ggggcgtgga acggccgacg accccgacgt cccaggtgtc
     4141 ggacccgagg tgccggacgg tctcca
//
LOCUS       DAOPQX010000342         4136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_23409_length_4136_cov_3.999755, whole genome shotgun sequence.
ACCESSION   DAOPQX010000342 DAOPQX010000000
VERSION     DAOPQX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23409_length_4136_cov_3.999755"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKK95_14660"
     CDS             complement(<1..645)
                     /locus_tag="PKK95_14660"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOC19510.1"
                     /translation="MSVTSAGNQTAAEAGASPVIHGPQVRRRFWHLPEDLLGVLCLVA
                     MVVLPLVEIVGRPLLGIGVPGSVGLVQHLTMWVAFVGAMVASRQRRHLALATATFLPT
                     HLRGVADVVAAAATASVGAVLAYTSAQMVYYERLSVQTLTGGIPIWVAGGGEKVTLRI
                     AAQYAQYTNFAGTPDEFEAKSALLKVHCDDLGTDFGAITRSANYNTVIGATEAEV"
     gene            complement(692..1693)
                     /gene="dctP"
                     /locus_tag="PKK95_14665"
     CDS             complement(692..1693)
                     /gene="dctP"
                     /locus_tag="PKK95_14665"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOC19511.1"
                     /translation="MRRLSLAAAAAACFFALAAPAAAQQLKVGTVAPEGSPWHDMLLD
                     LNARWKKVSGGKISLRIYPSGTQGDEPDMIRKMRIGQLHAAALTSVGMEAIATEMNAL
                     SIPFLFESWEEVDYVRDRLGPRLKKSLEDKGFVVLTWGDAGWVHFFTVTPAPRPADMQ
                     KLVLFTWGNDPRTEEMYKDAGFRVMPLASTEILQSLQTGKIQAFPAPPIAALASQWFG
                     AAKNMTPVKFAPVIGGAIISKAAWDRIDPALRPQLLEEAEEVGRIYKPRIRKLEGDAI
                     AAMQKYGLKITPITPEIEREWKQTAEKVYPRLRGGYLRAQDFDEVTGLVKEYRSKKK"
     gene            complement(1693..2607)
                     /locus_tag="PKK95_14670"
     CDS             complement(1693..2607)
                     /locus_tag="PKK95_14670"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOC19512.1"
                     /translation="MSALRGAAVALTALMLLGGCSIKKMAIKTVADSMAESSANYTSD
                     EDPELIREALPFGLKTLEGLAETLPNHRPLLRSLASGFTSYAVGFLVPEIRPLEETDL
                     ERSRELRVRSRLMLLRARDYALRSLEVEYPGFRAALFADAKTAVERVKVADVPDLYWA
                     AAAWGSAVSQGKDQMDLVAEVPIVEALIRRALALDESWEQGSLHEFMIVFESRGEASG
                     GSYQRARQHFDRAMALSNGRKIGPLVSLAENVSVYTGNRKEFDELLARALEFDVNSAP
                     ESRLINILGQRRARQLQAMADELFPEDL"
     gene            complement(2656..3996)
                     /locus_tag="PKK95_14675"
     CDS             complement(2656..3996)
                     /locus_tag="PKK95_14675"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC19513.1"
                     /translation="MPSPEAAAPPAPGLRQSLTLFDVTMIAIGGTIGSGIFLTPSLIA
                     QALPSPWLILGAWLVGGLMALAGALTFSELAGQMPHAGGQYVYLKEAYGPLVGFLFGW
                     AYFLVVNAGGLGALSVAFATYFGYFVPLGPAGAKAVAIGGLLALTAINVAGVKAGAFF
                     SDVFTVLKLAGIAGLVAVGLTFGSSQTSSGSLSAATLPGGLGAGLAAAMVGVLWSTGG
                     WQHATYASAEIRDARRTLPLAMTLGTAAVTLIYLAVNVAYLFLLTPSEMAASPRVAAD
                     AVSRALGPVGGSLISLAIFISTFGVVAIYTLTAPRIYFAMARDGVFFRRVAAVHPRFR
                     TPAFAIVAQSLWAVVLILFWGTFENLISYVVFTDWIFFGLAAAAVFVLRRRQPRAARP
                     VRVPLYPLTPAFFVAMSAWFVAVTLVERPAQAWAGLGLLALGVPAYYSWKRRTV"
BASE COUNT          668 a         1466 c         1431 g          571 t
ORIGIN      
        1 cacctcggcc tcggtcgccc cgatcaccgt gttgtagttg gcggatcggg tgatggctcc
       61 gaagtcggtg ccgaggtcat cgcagtgcac cttgagcagg gcggacttcg cctcgaactc
      121 gtcgggcgtg cctgcgaagt tcgtgtactg ggcgtactgg gccgcgatgc gcaacgtcac
      181 cttctcgcct ccgccggcga cccagatcgg gatgccgccg gtgagcgtct gcacgctcag
      241 ccgctcgtag tacaccatct gggccgacgt gtaggcgagc accgcgccga cgctcgccgt
      301 ggctgcggcg gccaccacat ctgcgacgcc gcggaggtgg gtgggcagga acgtggcggt
      361 ggccagcgcc aggtggcgcc gctgccgcga cgcgaccatc gcgcccacga aggcgaccca
      421 catcgtgagg tgctggacca ggccgaccga ccccggcacg ccgatgccga gcagcggccg
      481 gccgacgatc tcgaccagcg ggaggacgac catcgcgacg aggcagagca cgccgagcag
      541 gtcctcggga aggtgccaga agcgccggcg cacctgtgga ccgtggatga ccgggctcgc
      601 gcccgcttcg gcagccgttt gattgcccgc tgaagtcaca gacatcgctc cttgctcagg
      661 ccgccttctg cccccgcctg ccgccctccg cctacttctt cttcgaccgg tactccttca
      721 ccagccccgt gacctcgtcg aagtcctgcg cgcgcaggta gccgcctcgc agccgcgggt
      781 agaccttctc ggccgtctgc ttccactcgc gctcgatctc cggagtgatc ggcgtgatct
      841 tcagcccgta cttctgcatc gcggcgatcg cgtccccctc gagcttgcgg atcctcggct
      901 tgtagatccg gccgacctcc tcggcctcct cgagcagctg cggccgcagg gcggggtcga
      961 tccggtccca cgccgccttg gagatgatcg cgccgccgat gaccggcgcg aacttcaccg
     1021 gcgtcatgtt cttcgcggcg ccgaaccact gcgaggcgag cgcggcgatc ggcggggcgg
     1081 ggaaggcctg gatcttcccg gtctgcaggc tctggaggat ctcggtggac gccagcggca
     1141 tcacgcggaa ccccgcgtcc ttgtacatct cctcggtgcg tgggtcgttg ccccaggtga
     1201 agagcaccag cttctgcatg tcggcaggcc tcggggccgg cgtgaccgtg aagaagtgca
     1261 cccacccggc gtcgccccac gtcaggacga cgaagccctt gtcctcgagc gacttcttga
     1321 ggcgcggccc aagacggtcg cgcacgtagt ccacctcctc ccacgactcg aagaggaagg
     1381 ggatcgagag ggcgttcatc tcggtcgcga tcgcctccat gccgacgctc gtcagggccg
     1441 cggcgtgcag ctgcccgatc cgcatcttgc ggatcatgtc gggctcgtcg ccctgggtac
     1501 cgctggggta gatgcgcagg ctgatcttgc cgcccgacac cttcttccag cgggcgttga
     1561 ggtcgagcag catgtcgtgc cagggcgagc cctcgggggc gacggtcccg accttgagct
     1621 gctgcgcggc ggcgggggcg gcgagggcga agaagcacgc ggcggcggcc gccgccaggg
     1681 aaagacgtct cattacaggt cctccgggaa gagctcgtcg gccatcgcct gcagctgccg
     1741 cgcccggcgc tggccgagga tattgatcag gcgcgattcg ggcgccgaat tgacgtcgaa
     1801 ctcgagcgcg cgggcgagca gttcgtcgaa ctccttgcgg ttccccgtgt agaccgagac
     1861 gttctcggcc agcgacacga gagggccgat cttccggccg ttcgagagcg ccatcgcgcg
     1921 gtcgaagtgc tggcgggcac gctggtagct gccgcccgat gcctcgccgc ggctctcgaa
     1981 cacgatcatg aactcgtgga gcgacccctg ctcccagctc tcgtcgagcg ccagggcgcg
     2041 gcggatcagc gcctcgacga tcggcacctc ggccaccagg tccatctggt ccttaccctg
     2101 ggaaaccgcc gatccccacg ccgccgccgc ccagtacaag tcgggcacgt ccgcaacctt
     2161 cacgcgctcg accgccgtct tcgcgtcggc gaacagggcc gcccggaagc cggggtactc
     2221 gacctcgagc gagcgaagcg cgtagtcgcg agcgcgaagg agcatcaggc gcgatcgcac
     2281 ccggagctcg cgcgaccgct ccaggtccgt ctcttcgagc ggccggatct cgggcaccag
     2341 gaagccgacc gcgtacgacg tgaacccgct ggcgagcgag cgcaacaggg ggcggtggtt
     2401 ggggagcgtc tcggccagcc cctccagcgt cttgaggccg aagggcagcg cctcgcggat
     2461 cagctccgga tcctcgtccg aggtgtagtt ggcgctgctt tccgccatgg agtcggccac
     2521 cgtcttgatg gccatcttct tgatcgagca gccgccgagc agcatcaggg cggtcagggc
     2581 caccgccgcc ccccgcagcg cactcatcga cgttcctcca ggacctgggg ggagtgaaga
     2641 agaggactca acaggttaca ccgttcggcg cttccaggag tagtacgccg gcacgcccag
     2701 ggccagcagg cccagccccg cccaggcctg cgcgggacgc tcgacgagcg tcaccgcgac
     2761 gaaccacgcc gacatcgcga cgaagaacgc cggcgtgagc gggtacagcg gcacgcgaac
     2821 cggccgcgcg gcccggggct ggcgcctccg gaggacgaac accgcggcgg ctgcgaggcc
     2881 gaagaagatc cagtcggtga acacgacgta cgagatcagg ttctcgaagg tcccccagaa
     2941 gaggatcagc accaccgccc acagcgactg cgccacgatc gcgaaggcgg gtgtccggaa
     3001 ccgcgggtgc accgccgcga cgcgccggaa gaagacgccg tctctcgcca tcgcgaagta
     3061 gatgcgcggg gcggtcagcg tgtagatggc gaccaccccg aaggtcgaga tgaagatcgc
     3121 cagcgagatc aggcttcccc ccaccggccc gagcgcccgg ctcaccgcgt cggcggccac
     3181 ccggggcgac gccgccatct cggacggggt cagcaggaac aggtacgcga cgttcaccgc
     3241 gaggtagatc agcgtgaccg cggccgttcc aagcgtcatc gccagcggga gcgtccgccg
     3301 ggcgtcgcgg atctccgccg aggcgtaggt ggcatgctgc cagccgcccg tggaccagag
     3361 gacgccgacc attgccgccg ccagcccggc gccgagcccc cccggaagcg tcgcggccga
     3421 gagcgagcca ctgctcgtct ggctcgatcc gaacgtgagg ccgaccgcca ccaggccggc
     3481 gattcccgcc agcttcagca ccgtgaagac gtccgagaag aaggcaccgg ccttgacccc
     3541 ggcgacgttg atggcggtca gggccaggag ccccccgatg gcgaccgcct tcgcccctgc
     3601 cgggccgagg ggcacgaagt aaccgaaata ggtcgcgaag gcgacgctca gcgcgccgag
     3661 gccgcccgcg ttgaccacga ggaagtaggc ccacccgaac aggaagccca cgagcgggcc
     3721 gtaagcctcc ttgaggtaga cgtactggcc gccagcgtgg ggcatctggc ccgccagctc
     3781 ggagaaggtg agggccccgg cgagcgccat cagcccgccg accagccacg ctcccaggat
     3841 gagccacggc gagggaagtg cctgggcgat gagcgacggg gtgaggaaga tccccgaccc
     3901 gatcgtcccg ccgatcgcga tcatcgtgac gtcgaagagc gtgagcgact gccgcagccc
     3961 gggtgccggc ggcgcggcgg cttcaggaga gggcacgccg gatggctgcc tcgaggtcgg
     4021 tgggcaggca gggcttcacc agcacgtcgc gcgccccggc cgcgcgaacc cccgacagca
     4081 ggtcctggtc gccgctgccg ctcacgacga tgagggcctc gaggccggga aggccg
//
LOCUS       DAOPQX010000343         4109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_23590_length_4109_cov_3.826098, whole genome shotgun sequence.
ACCESSION   DAOPQX010000343 DAOPQX010000000
VERSION     DAOPQX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23590_length_4109_cov_3.826098"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PKK95_14680"
     CDS             <1..1671
                     /locus_tag="PKK95_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19514.1"
                     /translation="ALDGRRSLPGEGSPAADVTAAWLVRRFGAMPASVRRVRLDGLLF
                     TQRLAAQGEPGTGAGWLEAAATFPDWPLLFLMLEQLEATDPEDYRAAARAAAAVTAGQ
                     RTPEASRRLAAFQSGIALAVQIERAGTLRPPAVLELLRELFAVAPLDRARYAAGIADW
                     VERSLLARLGGSDSGEEARLLDALAGMVEGAGAAAPAVEWEGRPYVVDLARAARERLE
                     AVREGQGGPTLDEVLAQRRSGGNFEGPLADVLVALVYALVTDPDAPAVPGDGPWLRHD
                     FGLGDGVPSRAWRLVERSGSRGGRGSLLGLDRAMARDLLRPTMMGLPPRPPTLTLEAT
                     AGLAESVAALDPHRLDDEGRDLVAAALRRGRERLAEAAGRPGELDAVLASAGVDGIRR
                     RLARRAAAAGPSRVLESVSLGEVLELGQGGGPRVADAVLDAWGLAARPIDGSLRSQMP
                     RRLAWRERSGRPGSALVAAQVADLHLRIVEWLADRRLPGRLAPSILALAAWDLAMTAQ
                     VSGADDWLPVVRAAQAVPAQRLEDYVSALAAGGPLRPAGEGENGDEQG"
     gene            1700..2839
                     /locus_tag="PKK95_14685"
     CDS             1700..2839
                     /locus_tag="PKK95_14685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC19515.1"
                     /translation="MRHRAALVPIVVMATWVVVTASEPAVQILAPAQDSYVAGELVVR
                     AAIEPQGARVESVRFFGDGRLVCTVSRPPFECAWDAGPDIRQHDIRVVAAFTGGRRAV
                     ARVTTKGARFADTVDVDRVQVTAVVTDGRRYVRGLKRSDFRLYENGARVPIEHFSAEE
                     APLEVAVGVDISESMTGAIGQTKEFVKRFLSQLRETDRVTVLAFNENQFLIARPSVDL
                     AARVAAVDALAPWGATALHDVIVRSFRTLGRQQGRRALVVFTDGEDTASRVSAQAVER
                     RTESSDAVLFMIGQGQAVESEALRSLCERLARSSGGQAFFPRRIEDLGEVFDRIAEEL
                     SNQYLMTFAPSARRDGAYQEVRVEVAGGHEVRARRGYRLEPRRSR"
     gene            2836..>4109
                     /locus_tag="PKK95_14690"
     CDS             2836..>4109
                     /locus_tag="PKK95_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC19516.1"
                     /translation="MRTRGILAVLALAAAGVSLFGAGAQQPTFRATTEVIVVDVSVVG
                     KDGEPVRGLQESDFSISVSGKPRRVRTLQFVDQASGAAPAGESPLRPVSSNAPEARGR
                     LVLILVDEGSIGFGVLPAAAQSIERLLAGLGPADKVGLASLPGPRMHVDFTRDRAKIV
                     QAIQRVPSGAGADVRLQRVSVAVAEAFAIERGDPDMIERVVERECPPSDPLTQRICAQ
                     SVGMQAHAVVRNEENRTAQFSSGLKGVLQQLATVEGPKVIFILSQGFPDPGAPHQLIT
                     AAPFAAAARATLFGLLLDRSMLDLSSRPGPAYMMISNEERGWARASLDSLTGAARGTT
                     VEITGSAELPFARLAREISGYYLVGVEAEPGDHDGRSRPIKVEVNRPGVTVRARREVV
                     TRPAVRDERKLLTEALASPMLATDVPLRVSTF"
BASE COUNT          496 a         1423 c         1628 g          562 t
ORIGIN      
        1 gccctggatg gccggcgctc gctcccgggc gaaggttccc cggcggccga cgtgaccgcg
       61 gcatggctgg tacggcggtt cggagcgatg cctgcctcgg ttcggcgcgt ccgcctcgac
      121 gggctgctct tcacgcagcg gctggccgcc cagggcgagc cgggcaccgg cgccgggtgg
      181 ctcgaggctg cggccacgtt ccccgactgg ccactcttgt tcctcatgct cgagcagctc
      241 gaggccaccg acccggaaga ctaccgcgcc gcggcgcggg cggcggcggc ggtgacggcc
      301 ggccagcgca cgcccgaggc gtcgcgccgg ctcgcggcct tccagtcggg gattgcgctg
      361 gcggtccaga tcgagcgcgc cggcacgctg aggccgccgg cggtgctcga gctgctccgc
      421 gagctgttcg ccgtcgcacc gctcgaccgg gcccgctacg cggccggcat cgccgactgg
      481 gtcgagcgct cgctcctcgc ccggctcggc ggctcggatt ccggcgaaga ggcgcgattg
      541 ctcgacgcgc ttgccggcat ggtggagggc gcgggcgcgg cggccccggc tgtggagtgg
      601 gaaggccggc cgtacgtcgt ggacctcgca agagccgccc gggagcgact cgaggcggtt
      661 cgggaggggc agggcggccc gacgctcgac gaggtgctcg cacagaggcg ttccggcggc
      721 aacttcgagg gtccgttggc cgacgtgctg gtcgcactgg tctacgcgct cgtcaccgat
      781 cccgatgctc cggccgtgcc tggcgacggc ccctggctcc ggcacgattt cggcctgggc
      841 gacggcgttc cgtcgagagc ctggcggctg gtcgagcgaa gcggcagccg cggcgggcgc
      901 ggatcgctcc ttggcctcga ccgcgcgatg gcgcgcgacc tcctccgccc gacgatgatg
      961 gggctcccgc cccggccgcc gaccctcacc ctcgaggcga cggccgggct ggccgagtcg
     1021 gtggcggccc tcgacccgca ccggctcgat gacgagggac gcgacctggt ggccgcggcg
     1081 ctgcggcgag ggcgcgagcg gcttgccgaa gccgcgggac gcccggggga gctggatgcg
     1141 gtgctggcgt ccgcgggggt ggacggcatc cggcgccgcc tggcgcggcg tgccgctgcc
     1201 gccggcccgt cgcgcgtgct cgagagcgtg tcgctcggag aggtgctcga gctgggacag
     1261 ggcggcggcc cacgcgttgc agacgcggtg ctcgacgcgt ggggcctggc ggcgcggccg
     1321 atcgacggga gcctcaggtc gcagatgccg cgtcgcctcg cgtggcgcga gcgatcgggg
     1381 aggccggggt cggcgctggt ggccgcgcag gtcgccgacc tgcacctgcg catcgtggag
     1441 tggctggcgg atcggcgcct gccggggcgc ctcgcgccgt cgatcctggc gctggcggca
     1501 tgggacctgg ccatgaccgc ccaggtgtcg ggcgccgacg actggctgcc ggtggtgcgc
     1561 gccgcgcagg cggtgccggc acagcgcctg gaggattacg tttcggcact ggcggccggc
     1621 ggcccgctgc ggcccgccgg cgagggggag aacggcgacg agcagggctg aagccctgct
     1681 ccacgaggca ggagccggaa tgcgacaccg cgcggcgctg gtcccgatcg tcgtgatggc
     1741 gacgtgggtc gtggtgacgg cctccgagcc ggccgtccag atcctcgcgc cggcccagga
     1801 cagctacgtc gccggcgagc tggtggtgcg ggcggccatc gagccacagg gcgcgagagt
     1861 agaatccgtt cggttcttcg gtgacgggag gctggtttgc accgtcagcc ggccgccctt
     1921 cgagtgcgcg tgggacgccg ggcccgacat ccgccagcac gacatccggg tcgtggcggc
     1981 cttcacgggc gggaggcgcg cggtggcccg cgtcaccacg aagggggcgc ggtttgccga
     2041 cacggtggac gtggaccgcg tccaggtgac ggcggtggtc actgacggcc gccgctacgt
     2101 ccgcgggctg aagcgctccg acttccggct ctacgagaac ggcgcgcggg tcccgatcga
     2161 gcacttcagc gccgaggagg ctccgctcga ggtcgcggtg ggcgtggaca tcagcgagag
     2221 catgacgggc gcgatcggcc agaccaagga gttcgtcaag cgcttcctct cgcagctgcg
     2281 tgaaaccgac cgggtcaccg tgctggcgtt caacgagaac cagttcctga tcgcccgccc
     2341 ctcggtcgat ctggcggcgc gcgtcgcggc cgtggacgcg ctggccccgt ggggcgcgac
     2401 ggcgctgcac gacgtcatcg tgcgatcgtt ccggacgctc ggccgccagc aggggcggcg
     2461 cgcgctggtg gtgttcaccg acggcgagga cacggccagc cgcgtgagcg cgcaggcggt
     2521 ggagcggcgc accgagtcga gcgatgccgt gctcttcatg atcggtcagg ggcaggcggt
     2581 cgaatcggaa gcgcttcgct cgctctgcga gcggctggcg cgcagcagcg gcgggcaggc
     2641 gttcttcccg cggcgcatcg aggacctggg ggaggtgttc gaccggatcg ccgaggagct
     2701 gtcgaaccag tacctcatga ccttcgcccc gtccgcccgg cgcgacggcg cctatcagga
     2761 ggtccgggtg gaggtggccg gcgggcacga ggtgcgggca cggagagggt accggctcga
     2821 gccgaggagg agtcgatgag gacgcgggga atcctggcgg tcctggcgct ggcggcggcg
     2881 ggcgtcagcc tgttcggcgc cggcgcgcag cagccgacct tccgcgccac gaccgaggtg
     2941 atcgtggtgg acgtgtcggt ggtggggaag gacggcgagc cggtgagggg cctgcaggaa
     3001 tccgacttct cgatctccgt gtcgggcaag ccgcgccggg tgcgcacgct gcagttcgtg
     3061 gaccaggcga gcggggccgc tccagccggc gagtcgccac tgcggccggt gtcgtcgaac
     3121 gcgcccgagg cccgggggcg cctcgtgctg atcctcgtgg acgaggggag catcgggttc
     3181 ggcgtgctgc cggcggcggc gcagtcgatc gagcggctgc tcgccgggct cgggccggcc
     3241 gacaaagtgg ggctcgcctc gctgcccggc ccgcgcatgc acgtcgactt cacccgcgac
     3301 cgggcgaaga tcgtccaggc catccaacgg gtcccaagcg gcgccggcgc cgacgtccgg
     3361 ctgcagcggg tcagcgtcgc cgtcgccgag gccttcgcga tcgaacgggg cgatcccgac
     3421 atgatcgagc gggtcgtcga gcgcgagtgc ccgccttccg acccgctcac ccagcggatc
     3481 tgcgcccagt cggtcgggat gcaggcccac gccgtcgtgc gcaacgagga gaaccgcacg
     3541 gcccagttct cctcgggcct caagggggtg ctccagcagc tggccaccgt cgaggggccg
     3601 aaggtgatct tcatcctgtc gcagggcttc cccgatcccg gcgcgccgca ccagctgatc
     3661 accgcggccc cgttcgcggc cgccgcccgt gccaccttgt tcggcctgct gctcgaccgg
     3721 tcgatgctgg atctttcctc gcgccccggc ccggcgtaca tgatgatttc gaacgaggag
     3781 cgggggtggg cgcgcgcgtc gctcgactcg ctgacgggtg cggcgcgcgg caccacggtg
     3841 gagatcaccg ggtcggccga gctgccgttc gcccgcctgg cgcgcgagat ctccgggtac
     3901 tacctcgtcg gcgtcgaggc cgagcccggc gatcacgacg ggcggtcgcg gccgatcaag
     3961 gtggaggtga accggcctgg cgtcaccgtg cgcgcgcgcc gcgaggtcgt gacgaggccg
     4021 gccgtgcggg acgagcggaa gctgctgacc gaggccctcg cctcgccgat gctggcgacc
     4081 gacgtcccgc taagggtctc gaccttcaa
//
LOCUS       DAOPQX010000344         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_23703_length_4093_cov_4.778603, whole genome shotgun sequence.
ACCESSION   DAOPQX010000344 DAOPQX010000000
VERSION     DAOPQX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23703_length_4093_cov_4.778603"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="PKK95_14695"
     CDS             complement(<1..1994)
                     /locus_tag="PKK95_14695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC19517.1"
                     /translation="MLRFSRAASSDGVISLGLSGPNPSCRRLVVILVRPSRYDDEGYV
                     VRHFRGVLPSNTLSCLNSLTEDAVRTGAIGDLEVHVEVLDEIVSRVDPARLGRRFRRP
                     DTSVVVGLVGVQTNQFPRALDLARQFRAEGFAVMIGGFHVSGAIAMARGAMPPECQEA
                     IDAGVTLVLGEVEDRWAGLLRDAAAGRLRPVYDFLAEKPDLRDKPLPRASRRTQRKFT
                     MQAGGTIDAGRGCPFSCSFCTIINVQGRKMRCRSAAQVLAQIRANYPREAGGPGVRHY
                     FFTDDNFSRNPEWEAIFDGLIELREAHGISVDFMMQVDVLAPRVPRFVEKAARAGCVQ
                     VFIGLETIRGDNLDAGGKRQNKAVDYREMIAAWHRQGVVCHAGYIIGFPNDTYERVME
                     DVRTLRDELLVDQASFFMLTPLPGSEDHHRACDTGVPMDPDYNNFDSFHATTPHPLMT
                     ADEWTRAYRDAWKAFYSFDPMRRTLLRQNPHTYWGVLKNQIWYRASMAEGGHPMMTGF
                     FRLKDRQSRRPSFPLETRWAFTRRRVREIAYILREYARLYLEMQDLWLRTRIRRDDYA
                     FLGDLRKLAARSKQDAKVNWARVHLAVADRAAAWRAAHPTWRVPDRMAERLEAVRQAV
                     GNRAAAVGVSVGERGDAVRQSAMETSRRLGRRAAAYQAALA"
     gene            2159..3967
                     /locus_tag="PKK95_14700"
     CDS             2159..3967
                     /locus_tag="PKK95_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOC19518.1"
                     /translation="MPSETEKKPLSLPVAWREARDLMWAHRSRLAAGFVLMLVNRLAG
                     LVLPATSKFLIDDVIGRRQADLLVPLAAAAGVATLVQASTSFALSQVLGVAAQRAITN
                     MRRAVQAHVLRLPVAYFDQTKTGVLISRVMTDAEGIRNLVGTGLVNLLGGLLTAALGV
                     GILLYLNWRLTSLTLVILLVFGAGMALAFVRLRPVFRERGKINAEVTGRLGEALGGVR
                     IVKAYVAEKREDLVFTRGAHRLFRNVARSITGVSAITAFSTVVVGVIGVLMIVVGGRS
                     ILAGTMSLGDFVMYLFFTGLVAAPLVQIASVGTQITEAFAGLDRIREVRSIGGEDEQD
                     ASRRPLPSLQGAVEFGGVEFEYRPGVPVLRGVSFRAAAGTTTALVGSSGSGKSTLISL
                     VMAFNRPTAGRVLIDGADLASVRLRDYRAHLGVVLQENFLFDGTIAENIAYARPRATR
                     EEVLRVAAVAHCDAFVREFPDGYDTVVGERGVKLSGGQRQRIAIARAILADPRILVLD
                     EATSSLDSESEALIQDGLRSLRHGRTTFVIAHRLSTIRSADQILVLEGGEIVERGTHA
                     ELLALNGRYRQLHDRQYRLEQDRFVNPGEELPAEAS"
BASE COUNT          577 a         1397 c         1496 g          623 t
ORIGIN      
        1 gcgagggctg cctggtaagc ggccgcgcgc ctgccgaggc ggcggctcgt ctccatggcc
       61 gactggcgca ccgcgtcgcc ccgttcgcca accgagacgc cgaccgccgc cgcccggttg
      121 ccgacggcct gccgcaccgc ctcgagccgc tcggccatcc ggtctggaac cctccaggtg
      181 gggtgcgccg cccgccaggc cgccgcccgg tcggccacgg cgagatgaac gcgcgcccag
      241 ttgaccttgg cgtcctgctt cgagcgggcc gcgagcttcc ggaggtcgcc gaggaaggcg
      301 tagtcgtcgc gccggatccg ggtccgcagc cagaggtcct gcatctccag atacagcctg
      361 gcgtactccc gcaggatgta ggcgatctcg cggacgcggc gccgggtgaa cgcccagcgc
      421 gtctcgagcg ggaaggaggg gcgccgcgac tgtcgatcct tgagccggaa gaagccggtc
      481 atcatcgggt gcccgccctc ggccatggag gcgcggtacc aaatctggtt cttcaggact
      541 ccccaatagg tgtgagggtt ctggcggagc agcgtccgcc gcatcgggtc gaacgagtag
      601 aacgccttcc aggcgtcgcg gtaggcgcgg gtccactcgt cagcggtcat caaggggtgc
      661 ggcgtggtgg cgtggaacga gtcgaagttg ttgtagtcgg ggtccatcgg aacgccggtg
      721 tcgcaagcgc ggtggtggtc ctccgagccg gggagcggcg tgagcatgaa gaacgacgcc
      781 tggtccacca gcagctcgtc gcgcagcgtc cgcacgtcct ccatcacccg ctcgtaggtg
      841 tcgttcggga agccgatgat gtagccggca tggcagacga ctccctgccg gtgccatgcg
      901 gcgatcatct cgcggtagtc gaccgccttg ttctggcgct tgccgccggc gtcgaggttg
      961 tcgccgcgga tcgtttccag gccgatgaac acctgcacgc agccggcgcg ggcggccttc
     1021 tcgacgaagc gcggcacgcg cggcgcgagg acgtccacct gcatcatgaa gtcgaccgag
     1081 atgccgtgcg cctcgcgcag ctcgatcaac ccgtcgaaga tcgcctccca ctcggggttg
     1141 cgcgagaagt tgtcgtcggt gaagaagtag tggcggacgc cgggcccgcc ggcttcacgg
     1201 gggtagttcg cccggatctg cgccagcacc tgcgcggcgc tccggcaccg catcttccgc
     1261 ccctgcacgt tgatgatggt gcagaaagag cagctgaacg ggcagccgcg cccggcgtcg
     1321 atcgtgccgc ccgcctgcat ggtgaacttg cgctgcgtcc gcctggaggc ccggggcagg
     1381 ggcttgtcgc gcaggtcggg cttttcggcc aggaagtcgt acacggggcg aaggcggccg
     1441 gcggccgcgt cgcgcagcag gccggcccac cggtcctcga cctcgccgag caccagcgtc
     1501 acgccggcgt cgatcgcctc ctggcactcg ggcggcatgg ccccgcgcgc catcgcgatg
     1561 gcgccgctca cgtggaagcc gccgatcatc acggcgaagc cctcagcgcg aaactggcgg
     1621 gcgaggtcga gggcgcgggg gaactggttg gtctggaccc ccacgaggcc caccacgacc
     1681 gaagtgtccg ggcggcggaa gcgccgcccc agccgcgccg ggtctacacg gctgacgatc
     1741 tcgtcgagta cctcgacgtg cacctcgaga tccccgatcg cgccggtgcg gaccgcgtcc
     1801 tcggtgagac tgttgaggca gctcagggtg ttgctgggaa gcacgccacg gaagtgccgc
     1861 accacgtagc cctcgtcgtc gtaacgcgac ggccgcacga ggatgaccac gaggcgtcgg
     1921 caggaggggt tgggaccgga caaacccagg gagatcacgc cgtcggatga cgcggcgcgg
     1981 ctgaagcgca gcattcgagg cctccggggg cacgcccggg cagacaaacg cccggcacgc
     2041 ggtggctgac gggattgggt gcctgtccgt gtgtccttca ccctacggca tcgaccgcgg
     2101 aacgtcaagg acccggcccg cgtggacttg gcggaccgtt cgccggtaca ttgaagggat
     2161 gccctcggag accgagaaga agcccttgtc gctgccggtg gcctggcggg aggcccgcga
     2221 cctgatgtgg gcgcaccgct cgcggctcgc agccgggttc gtcctgatgc tcgtcaaccg
     2281 gctcgccggc ctcgtgctgc cggccacctc gaagttcctg atcgacgacg tgatcggccg
     2341 gcggcaggcc gatctgctgg tgccgctggc ggcggcggct ggcgtggcga cgctcgtgca
     2401 ggcttcgacc tccttcgccc tctcgcaggt gctgggagtg gccgcccagc gcgcgattac
     2461 caacatgcgc cgcgccgtcc aggcgcacgt gctccgcctg ccggtggcct acttcgacca
     2521 gacgaagacg ggggtcctga tctcgcgcgt catgaccgac gccgagggga tccggaacct
     2581 ggtggggacg ggcctggtga acctgctggg cggcctgctc accgccgcgc tcggggtggg
     2641 catcctcttg tacctgaact ggcgcctgac ctcgctcacg ctggtcatcc tgctcgtgtt
     2701 cggggcgggc atggcgctcg ccttcgtgcg gctgcggccg gtgttccgcg agcgcggcaa
     2761 gattaacgcc gaagtgaccg gccggctggg cgaggcgctc gggggcgtgc ggatcgtgaa
     2821 ggcgtacgtg gccgagaagc gcgaagacct ggtgttcacc cgcggcgcgc accgcctctt
     2881 tcgcaacgtc gcccgatcga tcaccggcgt gtcggccatc accgccttct cgacggtggt
     2941 cgtcggggtg atcggcgtcc tgatgattgt cgtcggcggg cgatcgatcc tcgcggggac
     3001 gatgtcgctg ggcgacttcg tgatgtacct cttcttcacc ggcctggtgg ccgcccccct
     3061 cgtgcagatt gcgtcggtcg gcacgcagat caccgaggcg ttcgcgggcc tcgaccggat
     3121 ccgcgaggtc aggtcgatcg gcggggagga cgagcaggat gcctcgcggc ggccgctccc
     3181 gtcgctgcag ggcgcggtgg agttcggcgg ggtcgagttc gagtaccggc ccggcgtgcc
     3241 ggtgctgcgc ggcgtgtcgt tccgggcggc ggcgggcacc accacggcgc tggtcgggtc
     3301 gagcggatcg gggaagagca cgctgatctc gctcgtgatg gcgttcaacc ggccgaccgc
     3361 gggccgcgtg ctgatcgacg gcgccgacct cgcctcggtg cggctgcgcg actaccgggc
     3421 tcacctcggc gtcgtcctcc aggagaactt cctgttcgac gggacgattg ccgagaacat
     3481 cgcgtacgcg cggccgcggg ccacgcgcga ggaggtgctc cgggtggccg cggtcgccca
     3541 ctgcgacgcc ttcgtccgcg agttcccgga cgggtacgac accgtggtgg gggagcgggg
     3601 tgtgaagctg tcgggagggc agcgccagcg catcgcgatt gcgcgcgcca tcctcgccga
     3661 cccgcggatc ctggtcctcg acgaggcgac ctcgagcctc gactccgaga gcgaggcgct
     3721 catccaggac ggcctgcgct cgctgcggca cggccgtacg acgttcgtca tcgcccaccg
     3781 gctgtccacc atccgaagcg ccgaccagat cctcgtcctc gagggaggcg agatcgtcga
     3841 gcgggggacg cacgcggaac tgctggcgct caacggccgc taccggcagc tgcacgatag
     3901 gcagtaccgg ctcgagcagg accggttcgt gaacccgggc gaggaactgc ccgccgaggc
     3961 gtcgtagggg cgacccttgc ctgccgcgcc gaagccgtag gcgaaggcgg tgcctgccgc
     4021 gccgaagccg aacgcgaagg cgggtcgtcc tccggagtat gatcgaccgt tcgacccttg
     4081 aagcccccca gcc
//
LOCUS       DAOPQX010000345         4068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_23885_length_4068_cov_4.605532, whole genome shotgun sequence.
ACCESSION   DAOPQX010000345 DAOPQX010000000
VERSION     DAOPQX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23885_length_4068_cov_4.605532"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKK95_14705"
     CDS             complement(<1..209)
                     /locus_tag="PKK95_14705"
                     /inference="COORDINATES: protein motif:HMM:NF026110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19519.1"
                     /translation="MTERAIFITEEDFERLEGLLEGAARQGGRDRENVRRLAEELDRA
                     HVVPADSIPADVVTMDSEVALRNLDT"
     gene            514..2196
                     /locus_tag="PKK95_14710"
     CDS             514..2196
                     /locus_tag="PKK95_14710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC19520.1"
                     /translation="MPPDPSAGRAPRRDPHLAELLDFRPDQGIIRLHEQRVLVLSAAA
                     MGLLRKELIDTFGLEASRRVLLRFGFADGYHDAVNLRARSGWTDPLEGVRSGALLHTL
                     EGLVRAEVRRIEHDAQTGRFEQELVWHDSYEAEQHVHHYGKSRLPVCWSLAGYASGFV
                     SACLGTEIYFREVECAGTGAARCVAVGRDAAGWAAALQAIRADFQAADLGREVERLRE
                     AVHARLRELGRRERLLDRRERELNVLRGRVERHAAARHFVARSQAMRDVLELAARVAP
                     LDTTVLVQGESGTGKEFVVRMIHDQSPRAAAPFVSINCAALTETLLESELFGHVRGAF
                     TGAVRDKAGLFEVAGSGTIFLDEIGDIAPTLQAKLLRALQEREIRRVGAERAVKVRAR
                     VVAATNRDLRAAVEAGRFREDLYFRLAAFVIAVPPLRERTEDIPPLVQAFLSRAASRM
                     KKDVKAVSAEAMSALMAYRWPGNIRELEHAVERAVILANGPAIRPRDLPPEVIDKRRP
                     RRQDETLDIREREKAAIERALERFAGNRRKAAAALGISTVTLWRRMKQYGLS"
     gene            2379..3542
                     /locus_tag="PKK95_14715"
     CDS             2379..3542
                     /locus_tag="PKK95_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOC19521.1"
                     /translation="MDMIYLDNAATSFPKPEETYVFMDRFYRTHGVNPGRSGFDLCME
                     TGHLVDETRALLTRFFNGTDPNRLVFGYNSTDALNLALFGLLAPGDHAITTTLEHNAV
                     LRPLYHLEALGGVAVDYVRFDGRGLVDPGNIERAIRPNTKVVALNHASNVIGTVQPVA
                     EIGRICRERGVRLVVDASQTAGKIPIDVQAMHVDVLCFTGHKSLMGPMGIGGMYVQEG
                     VEIRHTRAGGTGVRSAHRAHLDEYPWRMEYGTPNVLGIAGLNAGVKWIESRGLEAIDA
                     HEMRLTRRLVEGLREIPGVRLYCQDDLENHIAVVLFNVEGFEAGNTGTILDVDHNIAC
                     RTGLHCAPLVHEQIGTAAIHGGVRFGIGPFNTDADIDAAIAGVREIAAARART"
     gene            complement(3557..>4068)
                     /locus_tag="PKK95_14720"
     CDS             complement(3557..>4068)
                     /locus_tag="PKK95_14720"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOC19522.1"
                     /translation="ALFYTSTVAGALLADVLARYGHLPLIVELKEADLELARAVVRLF
                     AAAAPSRACTGSFYGPPLREVRRLAPAVPTGASGWEVRMALYRSWLRWPSDGVPYRAL
                     QVPERREAHRIVSPRFVRAAHRAGVAVQVWVVDEPDRIRRLLGWGVDGIITDRPDVAA
                     AIVADLRRG"
BASE COUNT          594 a         1463 c         1454 g          557 t
ORIGIN      
        1 gtgtcgaggt tccgcagggc cacctcggaa tccatcgtga ccacgtcggc cgggatgctg
       61 tcggccggca cgacgtgcgc gcggtcgagc tcctcggcca ggcggcgaac gttctcccgg
      121 tcgcggccgc cctgccgggc cgcgccctcc aggagccctt ccagcctctc gaaatcctct
      181 tcggtgatga agatggcacg ttcggtcata tcggtatcgc tttcctggat cgcagccgcg
      241 ccggccggcc gcgtttcgag cggggatggg gccgggcaca cccggcctct cgtgaggggg
      301 agcaacaatt gggccggggc gggagcggcg cagagagccg tcccgtgggg aaattgccgc
      361 cggcccgggt cgcgggctcc gtgaaacgtt tcaaattgaa acgttcgttt cagtcgtgaa
      421 acgtttcaga cccgttgcag cggcgtttca ggggctcgcg ggccgacttt cgccgcggcc
      481 ggggcggccc ggaggttgcg tctcagcaac cgaatgcctc ccgatccctc cgccgggcgt
      541 gcgccccgcc gcgatccgca cctcgccgag ctgctcgact tccggcccga ccaggggatc
      601 atccgcctgc acgagcagcg ggtcctggtc ctcagtgccg ccgcgatggg gctgctgcgc
      661 aaggagctga tcgacacctt cggcctcgag gcgagccggc gggtgctgct gcgcttcggc
      721 ttcgccgacg gctaccacga cgcggtcaac ctgcgggcgc gatcgggctg gaccgatccg
      781 ctcgagggag ttcgctcggg ggcgctgctc cacacgctgg aaggcctcgt ccgggcggag
      841 gtgcggcgca tcgagcacga cgcccagacc ggccggttcg agcaggaact ggtgtggcac
      901 gactcctacg aggccgagca gcacgtgcac cactacggga agagccggct cccggtgtgc
      961 tggtcgctgg cggggtacgc gagcggcttc gtcagcgcct gcctggggac cgagatctac
     1021 ttccgcgagg tcgagtgtgc gggcaccggg gcggcccggt gcgtggccgt cggccgcgac
     1081 gcggcgggct gggcggccgc gctccaggcg atcagggccg acttccaggc ggccgacctg
     1141 ggccgcgagg tggagcggct gcgcgaggcg gtgcacgcgc ggctcaggga gctgggccgg
     1201 cgcgagcggc tgctcgatcg ccgcgagcgc gagctgaacg tgctgcgcgg gcgggtcgag
     1261 cgccacgcgg ccgcgcggca cttcgtggcg cggagccagg cgatgcgcga cgtcctcgag
     1321 ctggccgcgc gcgtcgcccc gctcgacacc accgtcctgg tgcagggcga gagcggcacg
     1381 ggcaaggagt tcgtcgtccg gatgatccac gaccagagcc cccgcgccgc ggcgccgttt
     1441 gtcagcatca actgcgcggc gctgaccgag acgctgctcg agtcggagct gttcgggcac
     1501 gtgcgcgggg cgttcaccgg ggcggtgcgc gacaaggcgg ggctgttcga ggtggcgggc
     1561 agcggcacca tcttcctgga cgagattggc gacatcgccc ccacgctgca ggccaagctg
     1621 ctgcgcgccc tccaggagcg cgagatccgc cgcgtcggcg ccgagcgggc cgtcaaggtg
     1681 cgggcgcggg tcgtggcggc caccaaccgc gacctgcggg ccgccgtcga ggccggccgc
     1741 ttccgcgagg atctctactt ccgcctcgcg gccttcgtca tcgccgtgcc gccgctgcgc
     1801 gagcggaccg aggacatccc gccgctcgtc caggccttcc tgtcgcgggc ggcctcgcgg
     1861 atgaagaagg acgtgaaggc ggtctcggcc gaggcgatgt cggcgctgat ggcgtaccgc
     1921 tggccgggga acatccgcga gctggagcac gcggtcgagc gcgccgtcat cctggcaaac
     1981 ggcccggcca tccgtccgcg cgacctgccg cccgaggtga tcgacaagcg ccggccccgc
     2041 cgccaggacg agacgctcga catccgcgag cgcgagaagg cggcgatcga gcgcgcgctc
     2101 gagcggttcg ccggcaaccg gcgcaaggcg gccgccgccc tcgggatcag caccgtcacc
     2161 ctctggcgcc gcatgaagca atacggcctc tcctgacgac ccccacttcg acgtcgcgac
     2221 ctcgcggctt cgacttcgcg acctcgcaac ttcgacttcg cgacctcgcg gcttcgactt
     2281 cggtgacttc gtgacttcgc gacttcgtga cttcgtgact ccgtggactt gcaggagcgg
     2341 cgtggactag aatgcgtcgc cctcgtgagg cttccccgat ggacatgatc tacctcgaca
     2401 acgcggccac ctcgttcccg aagcccgagg agacctacgt cttcatggat cgcttctacc
     2461 gcacccacgg ggtgaacccc gggcggtcgg gattcgacct gtgcatggag acgggccacc
     2521 tggtggacga gacccgcgcg ctgctgaccc gcttcttcaa cggcaccgac cccaaccgcc
     2581 tggtcttcgg ctacaactcc accgacgcgc tgaacctcgc gctcttcggc ctcctcgccc
     2641 cgggcgacca cgcgatcacg accaccctcg agcacaacgc cgttttgcgg ccgctgtacc
     2701 acctggaagc cctgggcggc gtcgcggtgg actacgtgag gttcgacggc cgcggcctgg
     2761 tcgatcccgg caacatcgaa cgcgccatcc gccccaacac gaaggtggtg gccctcaacc
     2821 acgcgtcgaa cgtcatcggc accgtgcagc cggtcgccga gatcggccgc atctgccgcg
     2881 agcgcggggt gcggctggtc gtcgacgcct cgcagaccgc gggcaagatc ccgatcgacg
     2941 tgcaggcgat gcacgtcgac gtcctctgct tcaccgggca caagtcgctg atggggccga
     3001 tggggatcgg gggcatgtac gtgcaggagg gcgtggagat ccgccacacc cgcgcgggcg
     3061 gcaccggggt ccgctcggcc caccgggcgc acctcgacga gtacccgtgg cggatggagt
     3121 acgggacccc gaacgtcctc ggcatcgcgg gcctgaacgc cggcgtgaag tggatcgaaa
     3181 gccgggggct cgaggcgatc gacgcgcacg agatgcgcct caccaggcgg ctggtcgaag
     3241 ggctgcgcga gatcccgggc gtgaggctct actgccagga cgacctcgag aaccacatcg
     3301 cggtcgtgct gttcaacgtg gaggggttcg aggcgggcaa caccgggacg atcctcgacg
     3361 tcgaccacaa catcgcctgc cgcacggggc tgcactgcgc cccgctcgtg cacgagcaga
     3421 tcggcacggc ggccatccac ggcggcgtcc gcttcggcat cggccccttc aataccgacg
     3481 ccgacatcga cgcggccatc gcgggcgtcc gggagatcgc cgccgcgcgc gcaaggacct
     3541 gagagcggcc acgtgatcat ccgcgccgaa ggtccgcgac gatcgccgcc gccacgtcgg
     3601 gccggtcggt gatgatcccg tccacccccc accccagcaa gcgccggatc cggtcgggct
     3661 cgtccaccac ccacacctgc accgccacgc cggcgcgatg ggccgcccgg acgaagcggg
     3721 ggctcacgat ccggtgggcc tcgcgccgct cgggcacctg cagggcgcgg tagggcacgc
     3781 cgtcggaagg ccagcgcagc cacgagcggt acagcgccat ccggacctcc caccccgacg
     3841 cgccggtcgg caccgcgggc gcgagccggc gcacctcgcg gagcggcggc ccatagaacg
     3901 agccggtgca ggcgcgcgag ggcgccgcgg cggcgaacag ccgtaccacc gcgcgggcca
     3961 gctcgaggtc cgcctccttc agctcgacaa tcaggggcag gtggccgtag cgggccagca
     4021 cgtcggcaag cagcgcaccg gccacggtgc tggtgtagaa cagcgcct
//
LOCUS       DAOPQX010000346         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_23987_length_4055_cov_3.582250, whole genome shotgun sequence.
ACCESSION   DAOPQX010000346 DAOPQX010000000
VERSION     DAOPQX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23987_length_4055_cov_3.582250"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PKK95_14725"
     CDS             <1..646
                     /locus_tag="PKK95_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC19523.1"
                     /translation="GRDVGGFVEEARERIDAEVALPPGYFVTFGGQFEGQQRAMRHLA
                     VLMAIVLLVIFVILFSSFGSIWQAALVLLCVPTTLAGAMIALLVTGDSLNVSSTIGLI
                     ALFGVCAQNDIVLIGKINDLRRLGTPLREAVVQGSLLKLRPLLMTDLVMIVSVLPLVL
                     SQSTGSELHRPLAVVYVGGFLFALFLRRFFVPVLYEGMARLFERPSPAEVVEQA"
     gene            714..2750
                     /locus_tag="PKK95_14730"
     CDS             714..2750
                     /locus_tag="PKK95_14730"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC19524.1"
                     /translation="MMYPGSFATLVNLAGFLAALSLYAMLLAMVVRSSRTATVAGGTG
                     WPNRPARPHAWRHEPLLLAASLLGLAWNAVALRLYGLEEFGIGTPAGLRALGFTALGF
                     LPAVVVHSALRPALTERRDPTAAGVVGSAYLFSSAAAGLQIAAAVQGRPLPSPAALWL
                     LVAGFAALIVPLAFQAQRQVRARRALSLAALAVFGVSALHLAGHEALRDSWPLELVGH
                     HASLPLAVAILYQDYPFALADLFLKRALVVVMVVALALALYAEAFAWIVPAGPDAVTA
                     IVFAGLGVLVAFAYPWLRRAAYWLVDSVVLRRADYFALRAEIGQAIAAADSPEDVLRE
                     CCGRLGQAVAARLWRCWRLDADAPVPLDGCCATGGDAAPQPVLAAHAAWHDSNHAETV
                     HADATRTSAVAIVPTTEAPRYAFSLGSLAGGRRLLSDDLAMLGGVVQAAARRIDQVRL
                     ARERDDQRLREAEIRRLATEAELRALRAQINPHFLFNALTTVGYLIQTNPERALEKLV
                     QLTELLRRVLRSEGDFTTLGKELELIRLYLDIEHARFEERLRVEIDVPRGLHDIPMPA
                     LVLQPLVENAIKHGIAPSSGGGLVEVRAALDPPRPPSAAPTLTLTVHDTGCATAGTRP
                     ARGIGLRNVEDRLRHHFGDAASLRFESGREGTRVTLRIPARTAEAAPRAAEARS"
     gene            2747..3532
                     /locus_tag="PKK95_14735"
     CDS             2747..3532
                     /locus_tag="PKK95_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOC19525.1"
                     /translation="MSGGRLRVVIADDERPARSFLASLVRSFDDVELVGEAANGSEAL
                     QLIESLKPDLALLDLQMPELDGLSVARLLRGKHPPLVAFVTAYDEYAVRAFEVNAVDY
                     LLKPVSGARLRETLNRVQERLERGELFEESLRQVRAAATEYESSKQVGYVERLPVRHG
                     DHIVLLPVAQIASVEADGELLCVTTGRGERHTLAYRLKDLEERLDPARFIRLGRGELA
                     NIDMIVKVHPMPGGTYLVSLQNGQQLPVSRIQSRILRERLLKL"
     gene            complement(3636..3845)
                     /locus_tag="PKK95_14740"
     CDS             complement(3636..3845)
                     /locus_tag="PKK95_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19526.1"
                     /translation="MRGRRFADRSASGWGPAFLDRDGDGVCDHYPGPGRRGQGRRLGP
                     GANRVPGSGAGPAAAPGRGRGWRWR"
BASE COUNT          486 a         1510 c         1452 g          607 t
ORIGIN      
        1 cgggcgcgac gttggcgggt tcgtggagga ggcccgggag cggatcgacg ccgaggtggc
       61 cctgccgccg ggctacttcg tgacattcgg cgggcagttc gaggggcagc agcgtgcgat
      121 gcggcacctg gccgtgctga tggccatcgt gctcctggtc atcttcgtca tcctcttctc
      181 gtcgttcggc tcgatctggc aggccgcgct ggtgctgctg tgcgtgccga ccacgctggc
      241 gggcgcgatg atcgcgctgc tggtgaccgg cgactcgctc aacgtgtcgt cgacgatcgg
      301 gctgatcgcg ctcttcgggg tgtgcgccca gaacgacatc gtgctgattg gcaagatcaa
      361 cgacctccgg cggctcggca cgccgctgcg cgaggcggtc gtccaggggt cgctcctcaa
      421 gctgcgcccg ctcctcatga ccgacctcgt catgatcgtc agcgtgctgc cgctcgtcct
      481 gagccagagc accgggtctg agctgcatcg cccgctcgcc gtggtctatg tcggcgggtt
      541 cctgttcgcg ctgttcctgc gccggttctt cgtcccggtg ctctacgagg gcatggccag
      601 gctgttcgag cgcccgtcgc cggcggaagt ggtcgagcag gcgtagcagg ggcaaccccc
      661 gtggttgccc cccccggtcc tcgccgcacc gcggtataag atggccggca gggatgatgt
      721 accccggaag cttcgcgacg ctcgtgaacc tggccggttt cctcgccgcg ctctcgctgt
      781 acgcaatgct gttggccatg gtcgtccgca gctcgcgcac ggcgacggtg gccggcggga
      841 cggggtggcc gaaccggccc gcccggcccc acgcgtggcg ccacgagccg ctgctgctcg
      901 cggcctccct gctcggcctc gcctggaacg cggtggcgct gcgcctgtac gggctggagg
      961 agttcgggat cggcacgccg gccgggctgc gggcgctcgg gttcaccgcc ctcgggttcc
     1021 tgccggcggt cgtcgtgcac agcgcgctgc gccccgccct caccgagcgc cgcgatccga
     1081 ccgccgcggg ggtggtcggc agcgcgtacc tgttcagcag cgccgccgcg gggctgcaga
     1141 tcgcggccgc ggtccaggga cggcccctgc cgtcgccggc cgccctctgg ctgctggtgg
     1201 ccggctttgc cgcgctcatc gtgccgctcg cgttccaggc gcagcggcag gtccgcgcgc
     1261 gccgcgccct gtccctggcg gcgctcgccg tgttcggcgt ctcggccctt cacctggcgg
     1321 ggcacgaggc cctgcgcgac tcgtggccgc tggagctggt cgggcaccac gcctcgctgc
     1381 cgctcgccgt cgccatcctc taccaggact acccgttcgc cctggcggac ctgttcctga
     1441 agcgcgccct cgtcgtcgtc atggtcgtcg ccctcgcgct cgcgctttac gccgaggcgt
     1501 ttgcctggat cgtgcccgcc ggccccgacg ccgtcacggc gattgtcttc gcgggcctgg
     1561 gcgtcctggt ggcgttcgcg tacccgtggc tccgccgggc ggcgtactgg ctggtggaca
     1621 gcgtggtgct gcgcagggca gattacttcg ccctgcgcgc cgagatcggg caggcaattg
     1681 ccgccgccga ctctcccgag gacgtgctgc gcgagtgttg cggccgcctc gggcaggcgg
     1741 ttgccgcccg gctctggcgc tgctggcgcc tcgacgcgga cgcgccggtc ccgctcgacg
     1801 gctgctgcgc cactggcggc gacgcggccc cgcagcctgt cctggcggcc cacgccgcct
     1861 ggcacgactc caaccacgcc gagacggtgc acgccgacgc gacccgcacc agcgcggtgg
     1921 ccatcgttcc gaccaccgag gcgccccgtt acgccttctc gctgggcagc ctcgccggcg
     1981 gccggcgctt gctctccgac gacctggcga tgctcggggg cgtcgtccag gcggcggcgc
     2041 gacgcatcga ccaggtgcgc ctcgcccggg agcgggacga ccagcgcctc cgcgaggcgg
     2101 agatcaggag gctggcgacc gaggcggagc ttcgggcgct ccgcgcgcag atcaaccccc
     2161 acttcctgtt caacgccctg acgacggtcg ggtacctgat ccagacgaac ccggaacggg
     2221 cgctcgaaaa actcgtgcag ctcaccgagt tgcttcggcg ggtgctccgg tccgaggggg
     2281 acttcaccac gctggggaag gagctcgagc tgattcggct ctacctcgac atcgagcacg
     2341 cccgcttcga ggagcgcctg cgcgtcgaga tcgacgttcc gcgcggcctg cacgacatcc
     2401 cgatgccggc gctcgtcctg cagcccctcg tcgagaacgc gatcaagcac ggcatcgcgc
     2461 cctcgtccgg cggcgggctg gtcgaggttc gggcggccct cgacccgcct cggccgccct
     2521 cggcggcgcc gaccctgacg ctgaccgtgc acgacaccgg ctgcgcgacg gccgggaccc
     2581 gccctgcccg cggcatcggc ctgcgcaacg tggaggatcg gttgcgccac cacttcggcg
     2641 atgccgcctc gctccgcttc gagagcggcc gcgagggcac ccgtgtcacg ctgaggatcc
     2701 cggcccggac cgcggaggcc gcgccccggg cggcggaggc gcggtcgtga gcggggggcg
     2761 actgcgcgtc gtgatcgccg acgacgagcg gccggcgcgg tcgtttctcg cgtcgctcgt
     2821 gcgctcgttc gacgacgtgg agctggtggg ggaggccgcc aacggctcgg aggcgctgca
     2881 gctgatcgag tcgctgaagc ccgacctggc cctgctcgat ctgcagatgc ccgagctgga
     2941 cggtctcagc gtcgcgcggc tgctgagggg caagcacccg ccgctcgtgg ccttcgtcac
     3001 cgcgtacgac gagtacgcgg tccgcgcctt cgaggtgaac gcggtggact acctgctcaa
     3061 gccggtcagc ggggcaaggc tgcgcgaaac cctcaaccgg gtccaggagc ggctggagcg
     3121 gggcgagctc ttcgaggagt cgctgcggca ggttcgggcg gcggccaccg aatacgagtc
     3181 gtcaaagcag gttgggtacg tcgagcgcct tccggtgcgc cacggcgacc acatcgtgct
     3241 gctgcccgtc gcgcagatcg catcggtgga ggcggacggc gaactgctct gcgtcacgac
     3301 cggacgcggc gagcgtcaca cgctcgcgta tcggctgaaa gacctcgagg agcggctcga
     3361 ccccgcccgc ttcatcaggc tggggcgggg cgagctcgcc aacatcgaca tgatcgtcaa
     3421 ggtgcaccca atgcccggcg gcacctacct ggtgagcctg cagaacggcc agcagctgcc
     3481 ggtgagccgc atccagtcgc gcatcctccg ggagcgcctg ctcaagctct gaggcgcccc
     3541 atgcgagccg gcgagacagg tccgtccgac aggcgcactt tctgcgtgcc caacagcgag
     3601 gacgccctcg accgacgacc ggcgagacgc ttgtcctacc gccagcgcca gccccggccg
     3661 cgccccggcg ccgccgccgg tcccgcaccg gagcccggaa cgcggttcgc gcccggtcca
     3721 agccgccgcc cctggccccg ccgccccggc ccggggtagt ggtcgcagac accgtcgccg
     3781 tcgcgatcga ggaaggccgg cccccagccg ctcgccgagc ggtcggcgaa ccgccggcct
     3841 cgcacggccc agttgtcgca cgtgccgtcg ccgtcggtgt cgacgaacca acgtccccac
     3901 ccgcggcccc accctggcgg cggaggggcg tcgggctgtt cgcgccttcg acctgcgagc
     3961 cggccgcctc cggcggcgcc ggggcctgcg gggcccgtgc ctgcgcaagg gccggcgcgg
     4021 cacagagcac gagcacggca atcaccagta cgccc
//
LOCUS       DAOPQX010000347         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_24243_length_4022_cov_4.361230, whole genome shotgun sequence.
ACCESSION   DAOPQX010000347 DAOPQX010000000
VERSION     DAOPQX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24243_length_4022_cov_4.361230"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKK95_14745"
     CDS             complement(<1..381)
                     /locus_tag="PKK95_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19527.1"
                     /translation="MRLPVRPLLLAALLLFLVFGETPDRTRFWKELFNLGHAPLSGLL
                     ALIIRGWIANREGAAWRGAVARASVLAFIATVAIGSAVEALQVLQSDRNASWIDMGRN
                     TAGAASFLLLREAAVRWLGGASGAA"
     gene            505..2016
                     /locus_tag="PKK95_14750"
     CDS             505..2016
                     /locus_tag="PKK95_14750"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HOC19528.1"
                     /translation="MSRAIGFDNDKYLEEQSRAIVERVGQVGGRLYLEFGGKITFDYH
                     ASRVLPGYDPNVKIRLLQRLKDQAEIIVCIYAGAIERRKVRADFGLPYDLDALKLIDD
                     LRDWGLLVRAVVITRYEEQPSAKVFKARLERRGIAVYTHRFTRGYPTDVDRILSDEGY
                     GANTFVPAERPIVVVAGPGPGSGKLATCLSQVYHEHQRGQAAGYAKFETFPIWNLKLK
                     HPVNVAYEAATVELKDVNQIDPYHLAAYGEQAVNYNRDIDAFPLLARILGRITGETLY
                     RSPTDMGVNRAGFAIVDDKVVRAAAEQEVIRRYFRYACEYALGLVERDDLERSKLIMD
                     GLGLEPEHRPVVVAARGAAREAEAEGKGNEGVSCGAAIQLEDGSIVVGKNSPLMHAAS
                     AVVLNAIKQLSRIPDEIHLLTPNVVESIAGMKKDILRMKAVSLDLDETLIALGISAAS
                     NPAASAAVGRLTQLAGCEFHTTHIPTPGDEAGLRRLGLNATSEPQFATRALIL"
     gene            complement(2025..2543)
                     /locus_tag="PKK95_14755"
     CDS             complement(2025..2543)
                     /locus_tag="PKK95_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOC19529.1"
                     /translation="MITVYGADWCEDTQRALRHLRRLGVAYSYENVDRDPEALGRAKA
                     LNDGKRRTPTIDVNGTTLVEPTIGELTRVLEERHQVGPWQLKQLEVYNVGDLERLLRI
                     GAGLLAVTAAGRMRTAWRWPLAALGLMQTVSGAVGMCPTYTVLGVTSYGGPGDHPREA
                     ERRAWLTRVPGR"
     gene            complement(2601..3434)
                     /locus_tag="PKK95_14760"
     CDS             complement(2601..3434)
                     /locus_tag="PKK95_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19530.1"
                     /translation="MAFRRRRRAFRRTEWADDFGFAPHVSSSERRQRAQQAAAALRKA
                     GRELWPVSVEGREIASTFWGKAWCENLERYSDYANRLPRGRSYLRQGAVVDLQIAEGE
                     VGALVSGTRLYTVAIRIAPVKPKVWQAICQTCAGEIGSVVELLQGRLSDRVMEHLCRQ
                     ETGLFPTPREIRFSCSCPDAASMCKHVASALYGAGARLDEKPELLFRLRGVDEQELSA
                     RAASGIPLAAKAPPKGRMLDPTLIEELFGVAPDPPPPAAAPEARYREGRKRARKAKPA
                     R"
     gene            complement(3650..>4022)
                     /locus_tag="PKK95_14765"
     CDS             complement(3650..>4022)
                     /locus_tag="PKK95_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOC19531.1"
                     /translation="VERFQEDERVPFFVLSLKAGGTGLNLTAASHVVHVDRWWNPAVE
                     DQATDRAFRIGQTRNVLVHKFVCRGTVEERIDGLIASKRGLAKDILEGGAELLLTEMP
                     DEELLRLVALDARAAMTEDRK"
BASE COUNT          622 a         1438 c         1389 g          573 t
ORIGIN      
        1 ggcggcgccc gaggcgccgc ccagccagcg gaccgcggcc tcgcgcagca gcagaaagct
       61 cgcggcgcct gccgtgttcc gccccatgtc gatccagctc gcattgcggt ccgactgcag
      121 cacctggagc gcctcgaccg ccgaaccgat cgccacggtc gcgatgaacg ccagcaccga
      181 ggcgcgcgcc acggctcccc tccacgccgc gccctcgcgg ttggcgatcc agccccggat
      241 gatcaaggcc agcaggcccg acagcggggc gtgcccgagg ttgaacagct ccttccagaa
      301 acgggtgcga tcgggcgttt ctccgaacac gaggaacagg agcagcgcag cgagcaggag
      361 cggccggacg gggaggcgca cctgtcgagt atagcggctc gcgaacgccg ggcgggtttt
      421 cggctatgct ctcccgggcc gccgcggcgg attggctgct ccgggggttc gagcggcggg
      481 ccggcacact caggaggcga cacggtgtcc agggcgatcg ggttcgacaa cgacaagtac
      541 ctcgaggagc agagccgcgc catcgtcgag cgcgtcgggc aggtcggcgg gcggctgtac
      601 ctcgagttcg gcggcaagat cacgttcgac taccacgcct cgcgcgttct cccgggctac
      661 gacccgaacg tcaagatccg cctcctccag cggctcaagg accaggccga gatcatcgtt
      721 tgcatctacg cgggagcgat cgagcgccgg aaggttcgcg ccgacttcgg cctgccgtac
      781 gacctcgacg ccctcaagct gatcgacgac ctccgcgact gggggctgct ggtgcgggcg
      841 gtcgtcatca cgcgctacga ggagcagccg tccgccaagg tgttcaaggc gcgcctcgag
      901 cggcgcggca tcgcggtcta cacgcaccgc ttcacgaggg gctacccgac tgacgtggac
      961 cggatcctca gcgacgaggg atacggggcc aacaccttcg tcccggccga gcggccgatc
     1021 gtcgtcgtgg cgggtcccgg gccgggcagc ggcaagctgg ccacctgtct gtcgcaggtg
     1081 taccacgagc atcagcgcgg ccaggccgcc ggctacgcga agttcgagac gttcccgatc
     1141 tggaacctca aactgaagca cccggtcaac gtcgcctacg aggcggcgac cgtcgagctg
     1201 aaggacgtga accagatcga cccctaccac ctggccgcgt acggcgagca ggcggtgaac
     1261 tacaaccgcg acatcgacgc gttcccgctg ctcgcccgca tcctcggccg catcaccggc
     1321 gagacgctgt accgctcgcc caccgacatg ggggtcaacc gcgccggctt cgccatcgtg
     1381 gacgacaagg tggtgcgcgc ggcggcggag caggaagtga ttcggcgcta cttccgctac
     1441 gcctgcgagt acgccctggg gctcgtggag cgcgacgatc tcgaacggtc gaagctgatc
     1501 atggacggtc tcgggctcga acccgagcac cgcccggtgg tggtcgcggc ccggggcgcc
     1561 gcgcgcgagg ccgaggccga ggggaagggg aacgaagggg tgtcgtgcgg ggccgcgatc
     1621 cagctcgagg acggctcgat cgtcgtcggg aagaactcgc cgctcatgca cgccgcgtcg
     1681 gccgtcgtcc tgaacgcgat caagcagctg tcgcggattc ccgacgagat ccacctgctg
     1741 accccgaacg tcgtcgagtc aatcgcgggg atgaagaagg acatcctgag gatgaaggcc
     1801 gtcagcctcg acctcgacga gacgctgatc gccctcggga tcagcgcggc gtccaacccc
     1861 gccgcgagcg cggcggtggg gcggttgacg cagctggccg gctgcgagtt ccacacgacc
     1921 cacatcccga cgcccggcga cgaggcaggg ctgcgccgcc tcgggctcaa cgcgacgagc
     1981 gagccgcagt tcgcgacgcg cgcgctcatc ctgtagggcc cgggttaacg cccgggcacc
     2041 ctcgtcagcc aggcgcggcg ctccgcctcg cgcgggtggt cgccggggcc cccgtacgaa
     2101 gtcaccccga gcaccgtgta cgtcgggcac atcccgaccg cccccgacac cgtctgcatg
     2161 agtcccaagg cggccagcgg ccacctccag gcggttcgca tccgtcccgc ggcggtcacc
     2221 gccagcaggc cggcaccgat ccgcaggagg cgctcgagat cgccgacgtt gtagacctcg
     2281 agctgcttca gctgccacgg gccgacctgg tggcgctcct cgagcacccg ggtcagctcg
     2341 ccgatggtgg gctcgacgag cgtggtgccg ttcacgtcga tcgtcggggt ccggcgcttg
     2401 ccgtcgttga gcgccttggc gcgcccgagc gcctcgggat cgcggtcgac gttctcgtag
     2461 ctgtaggcca cgccgagccg ccgcaggtgg cgcagcgcgc gctgcgtgtc ctcgcaccaa
     2521 tcggcgccgt acacggtaat catgggagcc tccacgccgg cacccgcctt cgccgcttcg
     2581 cggcgacggc gaggccggcg ctaccgggcc ggtttggctt tccgcgcccg cttccttcct
     2641 tcacgatagc gcgcttcggg agccgccgcc ggcggaggcg gatcgggcgc aaccccgaac
     2701 agctcctcga tcagggtcgg atcgagcatg cggcccttcg gcggcgcctt ggcggcgagc
     2761 gggatgccgc tggccgcgcg ggcgctcagt tcctgctcgt ccacgccgcg gaggcggaac
     2821 agcagctcgg gcttctcgtc cagacgtgca ccggcgccgt acagagccga ggcgacgtgc
     2881 ttgcacatcg acgcggcgtc cgggcagctg cacgagaacc ggatctcgcg aggggtggga
     2941 aacaggccgg tctcctgccg gcacaagtgc tccatcaccc ggtccgagag gcggccctgc
     3001 agcagttcga ccaccgagcc aatctcgccc gcgcaggtct ggcagatcgc ctgccacacc
     3061 ttcggcttca ccggcgcgat ccggattgcg accgtgtaca ggcgggtgcc gctgacgagc
     3121 gcgcccacct cgccctcggc aatctgcagg tcgacgaccg cgccctgccg gaggtacgag
     3181 cggccgcggg gaagccggtt ggcgtagtcg ctgtagcgct cgaggttctc gcaccaggcc
     3241 ttgccccaga aggtggaggc aatctcgcgc ccctcgaccg agacgggcca cagctcgcgg
     3301 ccggccttgc gcagcgcggc ggcggcctgc tgcgcgcgct ggcgccgctc ggacgacgac
     3361 acatggggcg cgaagccgaa gtcgtcggcc cattccgtcc gccggaacgc cctcctcctg
     3421 cgccggaacg ccatgcgcct attgcagcac acaaccgccg cgatcgagat caccttgacc
     3481 acaaagacag gggcacaggg gtgctcccaa cggaagtgtc atggcgcgat cggggtcacg
     3541 ttcaacgcag agacgcggag gcgtcaatct ccgcgcctct gtggttgatc ttcggtctct
     3601 gcgttcgtct ctgcgtctcg gcgcctctgc gtctaacgtg gacagcgcgt cacttccggt
     3661 cctccgtcat cgcggcccgc gcgtcgaggg cgaccaggcg cagcagttcc tcgtccggca
     3721 tctcggtgag cagcagctcg gcgcccccct cgaggatgtc cttcgccagc ccgcgcttgg
     3781 atgcaatcag gccgtcgatc cgctcttcca cggtgccgcg gcagacgaac ttgtggacga
     3841 gcacgttgcg cgtctggccg atccggaagg cgcggtcggt ggcctggtcc tcgaccgccg
     3901 ggttccacca gcggtccacg tgcacgacgt gcgaggcggc ggtcaggttg agacccgttc
     3961 cgccggcctt gagcgacagg acgaaaaacg ggacgcgctc gtcctcctgg aagcgctcga
     4021 cg
//
LOCUS       DAOPQX010000348         4019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_24261_length_4019_cov_4.901362, whole genome shotgun sequence.
ACCESSION   DAOPQX010000348 DAOPQX010000000
VERSION     DAOPQX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24261_length_4019_cov_4.901362"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..2644)
                     /locus_tag="PKK95_14770"
     CDS             complement(347..2644)
                     /locus_tag="PKK95_14770"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOC19532.1"
                     /translation="MNTTNTTLPYKDSSRPVDERVEDLLARMTIEEKVAQMLCVWQQK
                     PAALVDDQGRFDEAKARERFGHGHGLGQVGRPSDAGGGLTARAMAETTNAIQRFFVEQ
                     SRLGIPVIFHEECLHGHAAREGTSFPQPIGLASTFDPDLVERVYTAVAAEARARGAHQ
                     ALAPDLDVARDPRWGRTEETFGEDPYLIGCMGVAAVRGFQGDGRFAGKTRVAATVKHF
                     AAHGEPEAGTNCAPVNVSMRVLREVCLPAFERAIVEGGALSVMPSYNEIDGVPSHANR
                     WLLGEVLRGEWGFRGFVVSDYFAIRELNERPELYGHHLAHDTREAAALAVRAGVDIEL
                     PDPDCYPALLELVREGVVPESLIDERVRPMLHAKFRLGLFDDPYVDPAEAERIVRDPA
                     HREVALEAARKTITLLKNDGVLPIDASAIRRIAVIGPNADRVMLGGYSGVPLQVGTAL
                     QGIRDRVPPGVEVLYHEGCKLTIGGSWNQDEVTPSDPDEDRRSIREAAEVAARADLVV
                     LAIGDNEQTSREAWALNHMGDRASLELFGLQDELVNAIAETGKPIVALLFSGRPASIR
                     NLAARAGAILELWYLGQESGRAIAEALFGDLNPGGKLPITIPRSVGHVPAFYNHKPSA
                     RRGYLFESSAPLFAFGYGLSYTTFAFSNLRLERDTIRAGESTHVLVDVTNTGSRRGDE
                     VVQLYVRDLVSSVTRPVKELKGFRRVTLDPGETTTVSLAVTPDALAFYDIDMKYKVEP
                     GDFRLMVGSSSRDEDLQTLTLHVTA"
     gene            complement(2832..>4019)
                     /locus_tag="PKK95_14775"
     CDS             complement(2832..>4019)
                     /locus_tag="PKK95_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC19533.1"
                     /translation="LVYVTPNVGAQGRLLYAYLTYTLVMMLYSANNTPYAALMGVMTG
                     DASERSSIAGYRFVGALVGQFVIQALPLPLVAKFGGGDSARGWAITMAIFGGLIILLN
                     LIAFSTTRERVQAAPGQRHPLKDDLKDVFTCRPWIVMFVLTLLVFTMLVVRGSSTNYL
                     FAYYMDQGQVREFLGKVGLAGLGGEATGWKALLDALGLLVKPDDSNAAAVGLSLFMVL
                     GSLVQIGGIICSKPLADRFGKKAVFSAGVAVTAAATLGVFLVGPSSVGLLFLLGILWA
                     VGWGPTVPLLWVMIADAADYSEWKTTRRATGFMFAGILFALKAGLSLGGALSAWVIDA
                     YGYVPNAVQTPHALLGIRLGASIYPGLALCLVLVCLALYPIGKTLNLRIQQELAARRA
                     TYA"
BASE COUNT          608 a         1415 c         1380 g          616 t
ORIGIN      
        1 ccaacctgcc ctacgactcg cagggcaacc tggtgaacca gaacggcgtc ggcacgatca
       61 gcagcgtcgg gcctgcccgc cagctccagc tgatgggccg gttccagttc taggctgacg
      121 gtttgggtgt cgccgtgccg gggaggcgcg gcgacgcagg agagcctcgc ttaggggcct
      181 ccacctcctg ggccccggag ggccggcacc ccgtcaccgt cagcggggtg tcggcccttt
      241 cttcgttaat cccacctgac agacgtggcg cccacgttca acccgccttc gcgcttcgcg
      301 cttcggcgcg gcgggatctt tgcgcctcca cggtgagggt ttccgttcac gcggtgacgt
      361 gtagcgtcag tgtctgcagg tcctcgtccc gcgacgagga tccgaccatc agccggaagt
      421 cgcccggttc gaccttgtac ttcatgtcga tgtcgtagaa ggccagcgcg tccggggtga
      481 ccgccaggga gacggtcgtc gtttcgccgg gatcgagcgt cacccggcgg aatcccttca
      541 gctccttgac cggccgggtg acgctgctca ccaggtcgcg cacgtaaagc tgcaccacct
      601 cgtcgccgcg ccgggaaccg gtgttcgtga cgtccacgag gacgtgggtc gactctcccg
      661 cccggatcgt gtcccgctcg agacgcaagt tcgagaaggc gaaggtcgta tagctgaggc
      721 cgtaaccgaa ggcgaagagc ggagcggacg attcgaacag gtagccgcgg cgagccgagg
      781 gtttgtggtt gtagaaagcc ggcacgtgcc cgaccgagcg cgggatcgtg atcggcagct
      841 tgccgcccgg gttgaggtcg ccgaacaggg cctcggcgat cgcccggccg ctctcctgtc
      901 ccaggtacca cagctcgagg atggcgccgg ccctggcggc caggttgcgg atcgaggccg
      961 ggcggccgct gaacagcagg gcgacgattg gcttccccgt ttcggcgatc gcgttgacca
     1021 gctcgtcctg cagcccgaag agctccaggc tcgcgcgatc gcccatgtgg ttgagggccc
     1081 aggcctcgcg cgaggtctgc tcgttgtcgc cgatggccag cacgacgaga tccgcgcggg
     1141 cggccacctc ggccgcctcg cggatggagc gccgatcctc gtcggggtcg ctcggcgtga
     1201 cctcgtcctg gttccacgac ccgccgatcg taagcttgca gccctcgtgg tagaggacct
     1261 ccacgcccgg gggcacgcgg tcccggatgc cctgcagggc cgttccaacc tggagcggca
     1321 cgccgctgta gccgccgagc atcacccggt cggcgttcgg cccgatcacc gcaatcctcc
     1381 ggatggcgga ggcgtcgatc ggcagcacgc cgtcgttctt gagcagcgtg atcgtcttgc
     1441 gcgccgcttc gagcgccacc tcgcggtggg ccgggtcacg gacgatgcgc tcggcctcgg
     1501 cgggatcgac gtacggatcg tcgaagagac cgagccggaa cttggcgtga agcatcggcc
     1561 gcacccgctc gtctatcagt gactcgggca cgaccccttc ccgcaccagc tccagcagcg
     1621 ccgggtagca gtcggggtcg ggcagctcga tgtccacccc ggccctgacg gcgagggcgg
     1681 cggcctcgcg ggtgtcgtgc gccaggtggt ggccgtacag ctcgggccgc tcgttcagct
     1741 cgcggatggc gaagtagtcg gacacgacga agccgcggaa cccccactcc ccgcgcagca
     1801 cctcgccgag gagccagcgg ttggcgtgcg acggcacgcc gtcaatctcg ttgtaggacg
     1861 gcatcacgct cagcgcgccc ccctcgacga tggcgcgctc gaaggcggga aggcagacct
     1921 cccgcaggac gcgcatcgac acgttgacgg gcgcgcagtt ggtgccggcc tccggctcgc
     1981 cgtgagctgc gaagtgcttc acggtggcgg ccacccgggt cttgccggcg aagcggccgt
     2041 cgccctggaa gccgcgcacg gccgcgaccc ccatgcagcc gatcaggtac gggtcttcgc
     2101 cgaaggtctc ctcggtgcgc ccccaccgcg ggtcgcgcgc gacgtcgagg tcgggagcga
     2161 gggcctggtg ggcgccgcgg gcgcgcgcct cggcggccac cgcggtgtac acgcgctcca
     2221 cgagatcggg gtcgaaggtc gaggccagcc cgatcggctg cgggaagctg gtcccctcgc
     2281 gcgcggcgtg gccgtgcagg cactcctcgt ggaagatcac cgggatgccg agccgcgact
     2341 gctcgacgaa gaaccgctgg atggcgttgg tggtctcggc catcgcgcgc gcggtcaggc
     2401 cgccgcccgc gtcgctcggt ctcccgacct gccccagccc gtgcccgtgg ccgaaccgct
     2461 cccgggcctt cgcctcgtcg aaccgcccct ggtcgtccac cagcgcggcc ggcttctgct
     2521 gccacacgca cagcatctgc gcgaccttct cctcgatcgt catccgcgcg aggaggtcct
     2581 cgacgcgctc gtccaccggc cgggatgaat ccttgtaggg aagcgtcgtg ttcgtcgtgt
     2641 tcatagcagg ctcgcgatcg ttgacgacag cgcacaaacg aaatgaacca caaagatcac
     2701 aaacgggctt ggccttcggg cggtcgtccc gcgctgctgc ccgggacgac ctgcggcgtg
     2761 gccccttctt tgtgcccttt gtggtacgcc ctctccgtgc cgtttctggt tccccgtcct
     2821 tcgtggtccc gtcacgcgta cgtcgcgcgg cgagccgcca gctcctgttg gatgcgcagg
     2881 ttgagcgtct tgccaatcgg gtacagggcg aggcacacga gcacgaggca cagggcgagg
     2941 cccgggtaga tgctcgcgcc cagccggatc ccgagcagcg cgtgcggcgt ctgcacggcg
     3001 ttcggcacgt aaccgtaggc gtcgatcacc cacgcgctca gcgccccgcc caggctcagc
     3061 cccgccttga gggcgaacag gatgccggcg aacatgaagc cggtggcccg gcgcgtcgtt
     3121 ttccactcgg agtagtcggc cgcgtcggcg atcatcaccc agagcagcgg aacggtgggg
     3181 ccccagccga ccgcccagag gatcccgagc aggaagagca gcccgaccga cgaggggccg
     3241 accaggaaca cgccgagcgt ggccgcggca gtcaccgcga cgccggcact gaacaccgcc
     3301 ttcttgccga accggtcggc cagcggcttg gagcagatga tcccgccaat ctgcaccagg
     3361 ctgccgagca ccatgaagag gctcaggccg accgccgccg cgttggagtc gtccggcttg
     3421 acgagcaggc cgagggcgtc cagcagcgcc ttccacccgg tcgcctcgcc gccaaggccc
     3481 gcgaggccta ccttccccag gaattcccgc acctgcccct ggtccatgta gtaggcgaac
     3541 aggtagttcg tggagctgcc acgcaccacg agcatcgtga acaccaggag cgtgagcacg
     3601 aacatcacga tccacgggcg gcacgtgaac acgtccttca ggtcgtcctt cagcgggtgc
     3661 cgctgccccg gcgccgcctg cacccgttcg cgcgtcgtgg agaaggcgat gaggttcagc
     3721 aggatgatca gcccgccgaa gattgccatc gtgatcgccc acccgcgcgc cgagtcgccg
     3781 ccgccgaact tcgcgacgag cggcagcggc agcgcctgga tgacgaactg gccgaccagc
     3841 gcgccgacga agcggtagcc ggcgatgctg ctccgctcgc tcgcgtcgcc ggtcatgaca
     3901 cccatgagcg cggcgtacgg cgtgttgttc gccgagtaga gcatcatcac gagggtgtag
     3961 gtcaggtagg cgtagagcag cctgccctgc gcgccgacgt tcggcgtgac gtagacgag
//
LOCUS       DAOPQX010000349         3995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_24445_length_3995_cov_4.521066, whole genome shotgun sequence.
ACCESSION   DAOPQX010000349 DAOPQX010000000
VERSION     DAOPQX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24445_length_3995_cov_4.521066"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="PKK95_14780"
     CDS             <1..1301
                     /locus_tag="PKK95_14780"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOC19534.1"
                     /translation="YRLVEGCVGVHVAAGRFGGITFGKDPAGRHWINTACQHVGASVW
                     WPNKDQQRDEVEGMRISVAIPDDLVDVSNGMFLGKTALGDGYTRWDWLVQYPINNYSV
                     SLNIGKYVHFTDADLAAGNQGSRQEGPAATKMDFWVLPESLEKARVQFAQAKTMMECF
                     EKYFGEYPFRKDGYKLIEVPYSGMEHQSAVTYGNRFTNGYLGRDWTGVGVSTKFDFII
                     IHESAHEWFGNSVTSIDVADEWIHEAWGTYLEAVYVEHVFGHDEAVKYINGYRAKVGN
                     REPIVQPRGLHQVPPQDMYFKGALFIHTLRTLVGDDARWWPLLRAFYQEFKYRNVSTE
                     QVVEFFNRRTGMNLTPLFDQYLRHAAPPRLELVFQDGGQVAYRWRADVEAFAMPVKVG
                     KPGRWQVLRPTTGWQVMTFQGGRDDLDAATDEYYVEVGKY"
     gene            1380..1598
                     /locus_tag="PKK95_14785"
     CDS             1380..1598
                     /locus_tag="PKK95_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19535.1"
                     /translation="MPDIRPPRTTQPDNAPPPGKRCPRCRVALEHERSYVTRGLTGVP
                     SFEHRFVCPACDTHYRWSSRDQRWREVH"
     gene            1704..>3995
                     /locus_tag="PKK95_14790"
     CDS             1704..>3995
                     /locus_tag="PKK95_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19536.1"
                     /translation="MQTFEKLGAFYLGKPYDLASGQRGDGWLLYDSRDLVTHAVCVGM
                     TGSGKTGLCLCLLEEAAIDGVPALVIDPKGDLGNLLLTFPNLRPEDFLPWVNPDEARR
                     KGLSVEEYAAQQAALWSNGLGEWGQDGARIARLREAADFAIYTPGSEAGLPLSILKSL
                     EAPRGEAAADPEVVRDRLSSTVTSLLGLLGIDADPVRSREHILLSTLVGAAWRNGEDL
                     GLADLIQRIQNPPIARVGVLDIEAFYPGKDRFQLAMAVNNLLASPGFEAWTNGEPLDV
                     QRLLYTPEGKPRISILSIAHLADAERMFFVSLLLNQVVGWMRAQGGTTSLRALLYMDE
                     IFGYLPPVANPPSKTPLLTLLKQGRAFGLGVVLATQNPVDLDYKALSNAGTWFLGRLQ
                     TERDKARVIEGLEGASAQAGQSFDRNRMEQVLAGLRSRVFLMNNVHEDEPVVFETRWA
                     MSYLRGPLGRQEIRKLTQSRQTTASAASAPPAPAPPAPPALVTAPSAPAPSAPAPSAP
                     APSAPAPSAPAPSAPAPSAPPALAPSAPPAPPAPPARQVLSVPVLPPSVSQFFLPVRG
                     AAPAGARLVYQPVLFGAAQVRFVDSKWKVDAAETLQATTPVADGPVAVDWAQAGECPF
                     APGDLETEPAPGASFAPLPASALKPKSYDAWQKAFVAWVYGSRKLGLFRSSALSLVSN
                     PGETEGDFRVRLQQAARERRDEAVADLRERYAKRLAQLQDRLRRAQEAREREQNQAST
                     QKMQTAISFGATLLGALMGRKKVT"
BASE COUNT          644 a         1445 c         1318 g          588 t
ORIGIN      
        1 tataccgtct ggtcgaggga tgtgtaggcg ttcatgtcgc cgccggccgc ttcggcggca
       61 tcacgttcgg caaggacccg gccggacgcc actggatcaa caccgcgtgc cagcacgtcg
      121 gcgcgagcgt ctggtggccg aacaaggacc agcagcgcga cgaggtcgag ggcatgcgga
      181 tcagcgtcgc catccccgac gacctggtgg acgtctcgaa cgggatgttc ctcggcaaga
      241 ccgccctcgg cgacgggtac acccggtggg actggctggt gcagtacccg atcaacaact
      301 acagcgtgtc gctgaacatc gggaagtacg tccacttcac cgacgccgac ctcgcggccg
      361 ggaatcaagg gtcacggcag gaaggccctg cagcaacgaa gatggacttc tgggtgctgc
      421 ccgagagcct cgagaaagcc agggttcagt tcgcccaggc gaagaccatg atggagtgct
      481 tcgagaagta cttcggcgag tacccgttcc ggaaggacgg ctacaagctg atcgaggtgc
      541 cgtactcggg catggagcac cagagcgcgg tgacctacgg caaccgcttc accaacggct
      601 acctcgggcg cgactggacc ggcgtcggcg tcagcacgaa gttcgacttc atcatcatcc
      661 acgagagcgc ccacgagtgg ttcgggaaca gcgtcacctc gatcgacgtg gccgacgagt
      721 ggattcacga ggcgtggggc acctacctcg aggcggtgta cgtggagcac gtgttcggcc
      781 acgacgaggc ggtgaagtac atcaacggct accgggcgaa ggtcggcaac cgcgagccga
      841 tcgtgcagcc gcgcggcctg caccaggtgc cgccgcagga catgtacttc aagggggcgc
      901 tcttcatcca caccctccgc acgctggtcg gcgacgacgc gaggtggtgg cctttgctgc
      961 gcgccttcta ccaggagttc aagtaccgca acgtctcgac cgagcaggtg gtggagttct
     1021 tcaaccggcg caccgggatg aacctgacgc cgctgttcga ccagtacctc cggcacgccg
     1081 cgccgccgcg gctcgagctg gtgttccagg acggcggcca ggtggcctac cgctggcggg
     1141 ccgacgtgga agcgttcgcg atgcccgtca aggtgggcaa gccgggccgc tggcaggtgc
     1201 tccggcccac gaccgggtgg caggtgatga ccttccaggg cggcagggac gacctcgacg
     1261 ccgcgaccga cgagtactac gtggaggtgg ggaagtatta gcggatgcta tactggcgca
     1321 ttgttgcgag cgcggtgcgc gcgccgtccg gctgaccgac cagagccgtt tttcgcccga
     1381 tgcccgacat ccgccctccc cgaacgacac agcccgacaa cgctccgccg cccggcaagc
     1441 ggtgcccccg ctgccgcgtc gcgctcgagc acgagcggag ctacgtgacg cgcggcctga
     1501 ccggtgtccc ctccttcgag caccgcttcg tgtgcccggc ctgcgacacc cactaccggt
     1561 ggtcgagccg cgaccagcgc tggcgcgaag tacactgaac cccacccccc tagcccctag
     1621 cccctagccc ctaaccccta ccccctggcc ccctagcccc tagcccctag cccctagccc
     1681 ctaaccccta gtagactccc gccatgcaga ccttcgagaa gctcggcgcc ttctacctcg
     1741 ggaagccgta cgacctggcg agcggccagc gcggcgacgg gtggttgctc tacgactcgc
     1801 gcgacctggt cacccacgcc gtctgcgtcg ggatgaccgg cagcggcaag acgggcttgt
     1861 gcctctgctt gctcgaggag gccgccatcg acggcgtccc cgcccttgtg atcgacccga
     1921 agggcgacct gggcaacctg ctgctcacct ttcccaatct ccgccccgag gatttccttc
     1981 cctgggtcaa cccggacgag gcccggcgca aggggctctc ggttgaggaa tacgcggcgc
     2041 agcaggcggc gctgtggtcg aacgggctcg gcgagtgggg gcaggatggc gcgcggatcg
     2101 cccggctgcg cgaggcggcc gacttcgcca tctacacgcc gggcagcgag gcgggcctcc
     2161 cgctctcgat cctgaagtcg ctcgaggcgc cgcgcggcga ggcggcggcc gaccccgagg
     2221 tggtgcgcga ccgcctctcc agcaccgtca ccagcctgct cggcctgctc ggcatcgacg
     2281 ccgacccggt gcggagccgc gagcacatcc tgctctcgac gctggtcggc gccgcctgga
     2341 ggaacggcga agacctcggc ctggccgacc tgatccagcg gatccagaac ccgccaatcg
     2401 cccgcgtcgg cgtgctcgac atcgaggcgt tctacccggg caaggaccgc ttccagctgg
     2461 ccatggcggt gaacaacctg ctcgcctcgc ccggcttcga ggcgtggacg aacggcgagc
     2521 cgctcgacgt gcagcgcctg ctctacacgc ccgaggggaa gccgcggatc tccatcctgt
     2581 cgatcgcgca cctggccgac gccgagcgga tgttcttcgt ctcgctgctg ctcaaccagg
     2641 tggtgggctg gatgcgggcg caggggggca ccacgagcct gcgggccctc ctctacatgg
     2701 acgagatctt cggctacctg ccgccggtcg cgaacccgcc gtccaagacc ccgctgctca
     2761 cgctgctcaa gcaggggcgc gccttcggcc tcggcgtggt gctggccacg cagaacccgg
     2821 tcgatctcga ctacaaggcg ctgtcgaacg ccggcacctg gttcctcggc cgcctgcaga
     2881 ccgagcgcga caaggcgcgc gtcatcgagg gactcgaggg agcctcggcc caggccggcc
     2941 agtcgttcga ccgcaaccgg atggagcagg tgctcgccgg gcttcgcagc cgggtgttcc
     3001 tgatgaacaa cgtccacgag gacgagccgg tcgtcttcga gacgcgctgg gcaatgtcgt
     3061 acctgcgcgg cccgctgggc cggcaggaaa tccggaagct cacgcaaagc cgccagacga
     3121 cggcctccgc agcctcagcc cccccggcac cagcgccccc ggcacctccg gcactcgtca
     3181 ccgcaccttc ggcaccagca ccctcggcac cagcaccctc ggcaccagca ccctcggcac
     3241 cagcaccctc agcaccagca ccctcagcac cagcaccctc agcacctcca gcactagcac
     3301 cctctgcacc tccagcacct ccggcacccc ctgcacgaca ggtgctgtct gtccccgtcc
     3361 tccccccctc ggtgtctcag ttctttctcc cggttcgcgg cgcggcgccc gccggcgcgc
     3421 gcctcgtcta ccagccggtg ctcttcggcg cggcgcaggt tcgcttcgtg gattcaaagt
     3481 ggaaggttga cgccgccgag acgctgcagg cgacgacgcc ggttgccgac ggcccggtgg
     3541 cggtggactg ggcgcaggcc ggcgagtgtc cgttcgcccc cggcgatctc gagacggagc
     3601 ccgcgcccgg cgcctccttc gcgccgcttc ctgcctccgc cctgaagccg aagagctacg
     3661 acgcgtggca gaaggccttc gtcgcgtggg tgtacggttc gcgcaagctg ggcctgttcc
     3721 gctcgtcagc gttgtctctc gtgtcgaacc cgggcgaaac cgagggcgat tttcgcgtcc
     3781 gcctccagca ggcggcgcgc gagcggcgcg acgaggcggt ggccgatctg cgggagcggt
     3841 acgcgaagcg gctggcgcag ctgcaggatc gcctgcgccg cgcacaggag gcgcgggagc
     3901 gcgagcagaa ccaggccagc acccagaaga tgcagacggc catctcgttc ggcgccacgc
     3961 tgcttggcgc gttgatgggc cgcaagaagg tcacc
//
LOCUS       DAOPQX010000350         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_24459_length_3994_cov_4.009393, whole genome shotgun sequence.
ACCESSION   DAOPQX010000350 DAOPQX010000000
VERSION     DAOPQX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24459_length_3994_cov_4.009393"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKK95_14795"
     CDS             <1..313
                     /locus_tag="PKK95_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19537.1"
                     /translation="LEAMGLGLLPPADERLWTLNAVRVPDGVDEARARRELLERFNME
                     IGSGLGPLAGKIWRVGLMGNGSTPQMVLLFLGALEAVLGSQGYRVPAGAGTAAAIGAL
                     S"
     gene            337..1098
                     /gene="mtgA"
                     /locus_tag="PKK95_14800"
     CDS             337..1098
                     /gene="mtgA"
                     /locus_tag="PKK95_14800"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOC19538.1"
                     /translation="MRGRRLPRIGQVALALLCAGFGYLAYVYLTLPDVSSLRTANPAT
                     TAFIELRVREARAKGHPARREQRWVGYSRISPTLKRAVLVAEDAAFWRHEGIDVDEIR
                     KSLERDLEQGRFSRGGSTITQQLAKNLYLTPSKNPLRKLKELLIARRLEAQLSKRRIL
                     ELYLNVIEWGDGVYGAEAAAREHFGKSAASLTADEAALLAGAIVNPRVHDPSKPTPRL
                     LGRQRLILSRMGVVSPPSDAAANPGPAAASEASGR"
     assembly_gap    1218..1317
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1318..1787)
                     /locus_tag="PKK95_14805"
     CDS             complement(<1318..1787)
                     /locus_tag="PKK95_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19539.1"
                     /translation="MINYTERISTLMRDIVARVPRLSYIDPGELLVFARYGRSGAEGA
                     FATCHCLSLPDSEPGYYYWRDRETGSITRRSRWFVTKSPSVSIQGRPIPYLVSFTLPR
                     FCNQTLTGARKEKYYRKGTPGWIAKLDTVVHELYHIDPAASGIRHVERADGLPSS"
     gene            complement(1851..2357)
                     /locus_tag="PKK95_14810"
     CDS             complement(1851..2357)
                     /locus_tag="PKK95_14810"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOC19540.1"
                     /translation="MEAPSAAGAGEAVASLALSLQGSPYVNGGAGLDGFDCSGFVQYV
                     FARSGVALPRSVLEQFEEGIGVEGAPEPGDLVFFAIDGGRVSHVGIATGPDTFVHAPS
                     SRGVVREDRLSAPYWSRRYAGARRVLPPIATQGRADTTAERRAQGRASPTAEPGTAEL
                     DTLSKRSL"
     gene            2599..3132
                     /locus_tag="PKK95_14815"
     CDS             2599..3132
                     /locus_tag="PKK95_14815"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOC19541.1"
                     /translation="MEHTLLLNASYEPLKVVPWRKAVTLWCQGKVEVISVYDREVRSV
                     SISFKLPSVIRLLRYVRIRRRFDYVPFSRANIYARDEYSCQYCGETLPATELTFDHVV
                     PVTQGGRKDWENIVTCCISCNREKGGRTPAEAGMRLLRRPVRPQAAPAIRITIGLRHT
                     PDSWRDYLYWNLELDES"
     gene            3216..>3994
                     /locus_tag="PKK95_14820"
     CDS             3216..>3994
                     /locus_tag="PKK95_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC19542.1"
                     /translation="MSQLFRLKSLDRMLGEAERGQSLKRTLGPFQLTILGIGAIVGAG
                     IFSTIGTAAAGGAGHTGAGPAIVVSFVLTAIVCGFAALCYAEFAAMVPIAGSAYTYAY
                     ATLGELVAWIIGWDLIIEYAVGNVAVAISWSGYFQELLRNMGVGFPAWLGTDYRSAAM
                     AAGQVAEAAAAGIDPASLGPAVTLAAQALAEAPRLAGLPIVFNLPAFAIVMLITWVLV
                     IGIKESAWVTTGTTFLNVGIILFFVVVGIFYVKPANWTPFFP"
BASE COUNT          540 a         1356 c         1405 g          593 t
ORIGIN      
        1 cctcgaggcg atggggctcg gcctgctgcc gccggcggac gagcgcctct ggacgctcaa
       61 cgcggtgcga gtccccgacg gcgtggacga ggcgcgcgcg cggcgggagc tgctcgagcg
      121 cttcaacatg gagatcgggt cgggactcgg cccgctggcg gggaagatct ggcgcgtcgg
      181 cctcatgggg aacggctcca cgccgcagat ggtgctgctc ttcctcggcg cgctcgaagc
      241 ggtgctcggg tcgcaggggt accgcgtgcc ggccggggcc ggcacggcgg cggccatcgg
      301 ggcgctctcg tgagggcccg gcgcacctcg aggtccttgc gcggacggcg gctgccgagg
      361 atcggccagg tcgccctcgc ccttctctgc gccgggttcg gctacctcgc gtacgtctac
      421 ctcacgctgc ccgacgtttc gtcgctgcgg acggccaacc ccgcgaccac cgcgttcatc
      481 gagctgcgcg tgcgcgaggc ccgcgccaag ggccaccccg cgcgtcggga gcagcggtgg
      541 gtgggctact cgcggatctc cccgacgctc aagcgcgcgg tgctggtcgc cgaggacgcg
      601 gcgttctggc gccacgaggg gatcgacgtg gacgagatcc gcaagtcgct cgagcgcgac
      661 ctcgagcagg ggcggttctc tcgcggcggc agcaccatca cccagcagct cgcgaagaac
      721 ctgtacctga cgccatcgaa gaaccccttg cggaagctca aggagctgct gatcgcacgc
      781 cggctcgagg cgcagctcag caagcggcgc atcctcgagc tctacctgaa cgtgatcgag
      841 tggggtgacg gcgtctacgg cgccgaagcg gcggcccgcg agcacttcgg caagtcggcc
      901 gcctcgctga ccgccgacga ggccgcgctg ctggctggcg ccatcgtcaa cccgcgggtg
      961 cacgacccct cgaagccgac gccgcggctg ctcgggcgcc agcggctgat cctctcccgc
     1021 atgggcgtgg tgtcgccccc gtccgacgcg gcggcgaacc caggcccggc agccgcatcc
     1081 gaggcgtccg gccggtaggg cgcggccgcc acgtccacgc cgcgcagccc gcccgcctcg
     1141 cttgtctcta caggactact cgccgcgtgc tgtcgatcag gaactgccgc accgcgagat
     1201 cgtcgtgggt gaagctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngac
     1321 gagggcagcc cgtccgcccg ttccacgtgc cggatgcccg aggcggccgg atcgatgtga
     1381 tacagctcgt ggacgacggt gtcgagcttc gcgatccagc ctggcgttcc cttgcggtag
     1441 tacttctcct tgcgggcgcc ggtgagcgtc tggttgcaga agcggggcag cgtgaacgac
     1501 acgaggtacg ggatcgggcg accctggatc gaaaccgagg gcgacttggt gacgaaccac
     1561 cgcgagcggc gggtgatcga tccggtttcc ctgtcgcgcc agtagtagta gccgggctcg
     1621 ctgtcgggca gggacaggca gtggcaggtc gcgaaggccc cctcggcgcc cgatcggccg
     1681 taccgtgcga agacgagcag ttcgccgggg tcgatatagg acaggcgcgg gacgcgcgcg
     1741 acgatgtcgc gcatgagagt gctgatgcgc tccgtgtagt tgatcattcg cgcatccgac
     1801 gtccgatccg gttgctgcgg aaggggggga agtgcgaaga agcgccgcga ctatagcgaa
     1861 cgcttcgaga gggtgtcaag ttcagccgtg ccaggttcag ccgttggcga ggcccgccct
     1921 tgcgccctcc gttcggctgt tgtatcggcg cgtccctgcg tcgcgatagg gggcagcacc
     1981 cgtcgggcgc cggcgtagcg gcgcgaccag tagggggccg acaggcggtc ctcgcgcacg
     2041 acgcctcggc tgctgggggc gtgaacgaag gtgtcgggcc cggtggcgat gcccacgtgc
     2101 gacacccggc cgccgtcaat cgcgaagaac accaggtcgc ccggctccgg cgccccctcc
     2161 accccgatgc cctcctcgaa ctgctcgagc acgctgcgcg gcagcgcgac gccggaccgc
     2221 gcgaagacgt actgcacgaa cccgctgcag tcgaacccgt ccaggccggc gccgccgttg
     2281 acgtacggcg agccctggag cgagagggcg aggctggcaa ccgcctcgcc cgcgccggcg
     2341 gcggagggtg cctccacggc cggatcaccg ccggcggggg agggcgcctc cggggccgga
     2401 tcgcctggga caggggaggc ggcgatgggc ggaggggcgc cgggataggg ccgcggggga
     2461 cgccgtgcgg cgccgcagcc ggccgccagc gccgcggcgc aggccagggc gatcgccgct
     2521 aacagcctgt tttccgagca ctttaggcgg gtcggccggt tgacagccct tgtggcctcg
     2581 gtcagaatac cctcggttat ggagcacacg ctcctgctga atgcctcgta cgagcccctg
     2641 aaagtcgtgc cctggcgcaa ggcggtgacg ctgtggtgcc aagggaaggt agaggtcatc
     2701 tccgtctacg accgcgaggt ccggtcggtc tccatcagct tcaagctgcc ctcggtcatc
     2761 cgcctgctcc gctacgtccg catccggcgc cgcttcgact acgtcccgtt ctcgcgcgcg
     2821 aacatctatg cccgcgacga gtactcctgt caatactgcg gtgagacgct gccggccacc
     2881 gagctgacct tcgaccacgt ggtgccggtc acgcagggcg gccgcaagga ctgggagaac
     2941 atcgtcacct gctgcatctc gtgcaaccgc gagaagggcg gccggacgcc ggccgaggcc
     3001 ggtatgcgcc tgctgcgccg gccggtgcgc ccgcaggcgg cgcccgccat ccgcatcacg
     3061 atcgggctgc ggcacacccc cgacagctgg cgcgactacc tctattggaa cctcgagctg
     3121 gacgagtcgt agcccgccgc ttttcccttg ccgcccgtcg agcctttcaa ggatgattca
     3181 cgcgccggcc gcgcgcgcgg ccggcaggag gtcgtgtgtc gcagctgttc cggttgaagt
     3241 ccctcgaccg catgctcggc gaggccgagc gcggccagtc gttgaagcgc acgctcgggc
     3301 cgttccagct caccatcctc gggatcggcg ccatcgtcgg cgcgggaatc ttctcgacaa
     3361 tcggcacggc ggcggccggg ggcgccggcc acaccggcgc ggggcccgcg atcgtcgtct
     3421 cgttcgtgct caccgcgatc gtgtgcgggt tcgcggcgct gtgctacgcc gagttcgcgg
     3481 cgatggtgcc gatcgccggg tcggcctaca cctacgccta cgccacgctc ggcgagctgg
     3541 tggcctggat catcggctgg gacctcatca tcgagtacgc ggtcggcaac gtcgcggtgg
     3601 cgatctcctg gtcgggctac ttccaggagc tgctgcgcaa catgggcgtc ggcttcccgg
     3661 cctggctcgg caccgactac cgatcggcgg ccatggcggc cggacaggtc gccgaggcgg
     3721 ccgcggcggg gatcgatccg gcctcgctcg gcccggccgt cacgctggcc gcgcaggcgc
     3781 tggccgaggc cccgcggctg gctggccttc ccatcgtctt caacctgccc gccttcgcga
     3841 tcgtcatgct gatcacgtgg gtgctcgtga tcggcatcaa ggagagcgcg tgggtgacga
     3901 cggggacgac gttcctgaac gtggggatca tcctgttctt cgtcgtcgtc gggatcttct
     3961 acgtcaagcc ggcgaactgg acgccgttct tccc
//
LOCUS       DAOPQX010000351         3941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_24892_length_3941_cov_5.847144, whole genome shotgun sequence.
ACCESSION   DAOPQX010000351 DAOPQX010000000
VERSION     DAOPQX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24892_length_3941_cov_5.847144"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PKK95_14825"
     CDS             complement(<1..867)
                     /locus_tag="PKK95_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HOC19543.1"
                     /translation="MLGSTTAVPSSWLAFATTVHAAMLVLRLHRGAPPRVLLLALPSL
                     ALAATPWALPTPGGVLAGLAAHLAWFMACEWLAPPPREAAPPPAACRPTASAVTPAKG
                     FVTVPVLAVQEQTRDIRTFRMARPPGFVLKAGQFLTVRIQTDGRPLTRCYSISSSPET
                     PGYLEISVKRQGPVSSLLHATLRPGVTLTIGPPAGPFVYPEADDRPIVLIAGGIGITP
                     LLSMLRHAVAAEPARRVTLIYSVRSLDDLAFGDELSLVARQHPQARVVVTLTAERHEG
                     YRHGRVDPAAILQ"
     gene            complement(1152..>3941)
                     /locus_tag="PKK95_14830"
     CDS             complement(1152..>3941)
                     /locus_tag="PKK95_14830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOC19544.1"
                     /translation="PGVAEVASIGGFVRQYQITVDPNKLAAYNLPLEEVVAAVRAGNN
                     EVGGRLLEWSGREYMVRGRGYARSLEEFEQIVVKAAPGGVPVLLRDVARLELGPDLRR
                     GVADLDGTGEAVGGIVVMRHGENALNVIRAVKQRIEDLGPSLPEGVEIVTTYDRSSLI
                     ERAIGTLRHELVIEMIIVSLVILVFLWHIPSAIVPIVTIPVSVLLAFIPLYYMGVTVN
                     IMSIAGIAISIGVLVDGAIVEVENAYNKLHRWQAEGRKGDFHQVRLEALTEVGPSVFF
                     SLLVIAVAFIPIFALVDQEGRLFKPLAYSKNLAMAIAAILAITLDPAMRMLFTRMDPL
                     RFRPRWLARAATTALVGTYHAEERHPISRAIFTVYDPACRFVLRYPRSVIAAALLAVA
                     VSIPIYFKLGSEFMPPLNEGTILYMPTTVPGISAAQAQDLMRRQDEALRRLPEVESVM
                     GKAGRAETSTDPAPFSMMETTIALKPREQWRRVERWYTHRLPEFTHALLRSIWPDRIS
                     WEDLVTQMDEAVRMPGVSNAWTMPIKGRIDMLTTGVRTPVGVKVMGADLATIERVGQR
                     IEEILRPVEGTRSVFAERVTSGNFVDIVPRRDDLARHGLTIDALQSVIMSAIGGENVS
                     TTIEGRERYPVNVRYPRELRDDVPRLRRVLVATPAGAQVPLEQLADIQVLTGPAMLRN
                     ENGFLTGYVFVDIAGRDVGSYVEEAKRLVGERLELPPGTVLQWSGQYENMQRVQDRLK
                     IVVPITLSLIFLLLYLNTRSAFKAGLVMLAVPFSAIGAIWLFYLLDYNVSIAAWVGMI
                     ALMGLDAETGVFMLLFLDLSYDEYAESGRLRSLADLDEAILHGAVKRARPKMMTVAAA
                     FMGLLPIMFSTSAGADVMKRIAAPMIGGLATSFLLELLVYPAAFKLWKLRAHPLLAKP
                     AEPGEPREAALSPAR"
BASE COUNT          632 a         1353 c         1389 g          567 t
ORIGIN      
        1 ttgcaggatg gctgccgggt ccacgcggcc atgccggtac ccttcgtggc gctcggccgt
       61 gagcgtgacg accacgcgcg cctgcgggtg ctgccgggcg acgagcgaca gctcgtcgcc
      121 gaaggcgagg tcgtcgagcg atcgcaccga gtagatcagc gtgacgcgcc gcgccggctc
      181 ggcggcgacc gcgtgccgca gcatgctgag aagcggagtg atgccaatgc cgcccgcaat
      241 caggacgatg ggccggtcgt cggcttcggg gtagacgaac gggccggcgg gcgggccgat
      301 ggtgagcgtg acgcccggtc gaagcgttgc gtgcagcagg ctcgagaccg gtccctggcg
      361 cttgaccgag atttccaggt acccgggcgt ctcgggcgag gagctgatcg agtagcagcg
      421 ggtgaggggc cgcccgtcgg tctgaatccg cacggtcagg aactggcccg ccttcaagac
      481 gaagccggga ggccgcgcca tgcgaaacgt ccggatgtcc cgcgtctgct cctgcacggc
      541 cagcaccggt acggtgacga atcccttggc cggcgtcacc gcgctggcgg taggacggca
      601 ggcagccggc ggcggagcgg cctcgcgggg cggcggcgcc agccactcgc aggccatgaa
      661 ccacgccagg tgcgcggcaa gtcccgccag cacaccacct ggcgtcggca gcgcccacgg
      721 cgtggcggcg agcgcgagcg atggaagggc gagcagcaag acgcgcggcg gggctccgcg
      781 atggaggcgc agcaccagca tcgccgcgtg gacggtcgtg gcgaacgcca accaggagga
      841 cggcaccgcc gtcgtcgatc cgagcatgtg tggtgcgccg cctgcagggc tgcaagggca
      901 ataccaaggg ctgagccggc cttgtcgccg tgaggatcaa gcttggcaac tggctgaacg
      961 gtaaggagtt gggaacagct cgccgggccg cggacggccg ggccgtcgag cgtcggcttg
     1021 cggtgaggat gtaaacgtta cgcaagccgc agcacgcggc gacgccgccg cccgctctcg
     1081 ccttcgcttc accggacgac gcgccgctcg ctctcgcttt caccggatgg cggcgccgcc
     1141 atccatctcc ttcaccgggc ggggctgagc gctgcctccc gcggttcacc gggctccgcc
     1201 ggtttcgcga gcaacgggtg cgcccggagc ttccaaagct tgaaggcggc agggtagacg
     1261 agcagctcca gcaggaacga ggtcgccagc ccgccgatca tcggggccgc gatccgcttc
     1321 atgacgtcgg cgccggccga cgtggagaac atgatgggca ggaggcccat gaaagcggcg
     1381 gcgacggtca tcatcttcgg gcgcgcccgc ttgaccgcgc cgtggaggat ggcctcgtcg
     1441 aggtcggcca ggcttcgcag ccgccccgac tccgcgtact cgtcgtacga gaggtcgagg
     1501 aacagcagca tgaagacgcc ggtttccgcg tcgagcccca tcagcgcgat catccccacc
     1561 caggcggcaa tcgagacgtt gtagtcgagc aagtagaaga gccagatcgc cccgatggcc
     1621 gagaagggaa cggccagcat caccaggccc gccttgaagg ccgaccgcgt gttcaggtag
     1681 agcagcagga agatcaacga cagggtgatc gggacgacga tcttcagccg gtcctgcacc
     1741 cgctgcatgt tctcgtactg gccgctccac tggagcacgg tgcccggcgg cagctcgagc
     1801 cgctcgccca ccagcctctt ggcctcttcg acatagctgc ccacgtcgcg gccggcgatg
     1861 tcgacgaaca cgtagcccgt gaggaacccg ttctcgttcc tcagcatggc ggggccggtg
     1921 agcacctgga tgtcggccag ctgctcgagc ggaacctgcg ccccggctgg cgtcgccacc
     1981 agcacgcgcc gcagccgcgg cacgtcgtcg cgcagctcgc gcgggtagcg cacgttgacg
     2041 gggtagcgct cgcgaccctc gatcgtcgtg gacacgttct cgcccccgat ggccgacatg
     2101 atcaccgact gcagggcgtc gatcgtcagg ccgtggcggg ccaaatcgtc ccgccgcggg
     2161 acgatgtcca cgaagttgcc gctggtgacg cgctcggcga agacgctccg cgtcccttcg
     2221 accgggcgca ggatctcctc gatgcgctgc cccacgcgct cgatggtggc caggtcggcc
     2281 cccatcacct tgacgccgac cggcgtgcgt acgccggtcg tcagcatgtc gatccgcccc
     2341 ttgatcggca tcgtccaggc gttgctcacg ccgggcatgc gcacggcctc gtccatctgg
     2401 gtgaccaggt cttcccacga gatgcggtcc ggccagatcg agcgcagcag ggcgtgggtg
     2461 aattcgggaa gccggtgcgt gtaccagcgc tcgacgcgcc gccactgctc gcgcggcttc
     2521 agcgcgatcg tcgtctccat catcgagaac ggcgccgggt cggtggacgt ttccgcccgc
     2581 cccgccttgc ccatcacgct ctcgacctcg gggaggcggc gcaacgcctc gtcctgccgc
     2641 cgcatcaggt cctgggcctg ggcggcggag atgccgggga cggtcgtcgg catgtagagg
     2701 atcgtcccct cgttgagcgg tggcatgaac tcggacccga gcttgaagta gatcgggatg
     2761 ctgacggcga cggcgagcag cgccgcggcg atgacgcttc tcgggtaccg gagcacgaag
     2821 cggcacgccg ggtcgtacac cgtgaagatg gcgcggctga tcgggtggcg ctcctccgcg
     2881 tgatacgtcc ccaccagggc ggtggtcgcg gcccgcgcca gccagcgcgg ccggaaccgc
     2941 agcgggtcca tgcgcgtgaa cagcatccgc atggccgggt cgagggtgat cgccaggatg
     3001 gctgcgatcg ccatcgcgag gttcttcgag tacgcgagcg gcttgaagag ccgcccctcc
     3061 tggtccacca gcgcgaagat cgggatgaag gccaccgcga tgacgagcag cgagaagaag
     3121 accgagggcc cgacctcggt gagcgcctcg agccgcacct ggtggaagtc gcccttccgc
     3181 ccctcggcct gccagcggtg gagcttgttg taggcgttct cgacctccac gatcgccccg
     3241 tccaccagca cgccgatcga gatcgcgatc ccggcgatcg acatgatgtt cacggtcacg
     3301 cccatgtagt acaggggaat gaaggcgagc agcaccgaga ccgggatggt gacgatcggc
     3361 acgatggccg acgggatgtg ccagaggaag acgaggatca cgagcgagac gatgatcatc
     3421 tcgatcacca gctcgtgtct cagcgtcccg atggcgcgct cgatgaggct ggaccggtcg
     3481 tacgtcgtaa cgatctcgac cccctccggc agcgaggggc ccaggtcctc gatccgctgc
     3541 ttgaccgcgc ggatgacgtt gagcgcgttc tcgccgtggc gcatgacgac gatgccgccc
     3601 accgcctcgc cggtgccgtc gaggtcggcg acgccgcgcc gcagatcggg tcccagctcg
     3661 aggcgggcca cgtcgcgcag cagcaccggc acgcctcccg gcgccgcctt cacgacgatc
     3721 tgctcgaact cctcgagcga gcgggcgtac ccgcgcccgc ggaccatgta ctcgcggccg
     3781 ctccactcga gcagccgtcc gcccacctcg ttgttccccg cccgaacggc cgcgaccacc
     3841 tcctcgagcg gcaggttgta ggcggcgagc ttgttcggat ccaccgtgat ctggtactgg
     3901 cggacgaagc cgccgatcga ggcgacctcg gcgacgccgg g
//
LOCUS       DAOPQX010000352         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_25074_length_3921_cov_4.846353, whole genome shotgun sequence.
ACCESSION   DAOPQX010000352 DAOPQX010000000
VERSION     DAOPQX010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25074_length_3921_cov_4.846353"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..193)
                     /locus_tag="PKK95_14835"
     tRNA            complement(118..193)
                     /locus_tag="PKK95_14835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Thr,seq:ggt)
     gene            complement(373..447)
                     /locus_tag="PKK95_14840"
     tRNA            complement(373..447)
                     /locus_tag="PKK95_14840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(413..415),aa:Gly,seq:tcc)
     gene            complement(628..714)
                     /locus_tag="PKK95_14845"
     tRNA            complement(628..714)
                     /locus_tag="PKK95_14845"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Tyr,seq:gta)
     gene            complement(761..837)
                     /locus_tag="PKK95_14850"
     tRNA            complement(761..837)
                     /locus_tag="PKK95_14850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Thr,seq:tgt)
     gene            complement(890..1636)
                     /gene="rlmB"
                     /locus_tag="PKK95_14855"
     CDS             complement(890..1636)
                     /gene="rlmB"
                     /locus_tag="PKK95_14855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOC19545.1"
                     /translation="MIIYGLNPVIEALRAGCVREIRVADRHDRRMRQLFELAAGAGVA
                     VRRAEPAELDRAARGGRHQGVVAEIDEARDYGVAELVEGAFGPALLVVLDGVEDPHNF
                     GAILRVADAAGADGVIRQTRRAAALDGAAAKASAGALAHVRVASVVNIARALEELREA
                     NVWTVGLAGDAPLPYSEVDLTQPSAIVLGAEGSGLRRLVRERCDLLASLPMRGHVDSL
                     NVSVAAGIVLFEALRQRAGTAREGQKAGQK"
     gene            complement(1648..3153)
                     /gene="gltX"
                     /locus_tag="PKK95_14860"
     CDS             complement(1648..3153)
                     /gene="gltX"
                     /locus_tag="PKK95_14860"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOC19546.1"
                     /translation="MRVRFAPSPTGHLHVGNARTALFNWLLARGSGGTFILRIEDTDR
                     ERSTSASEQAILEDLEWLGLEWDEGPGVGGDRGPYRQSERLDIYRAQADRLLAEGRAY
                     YCFCPEAQLKAEREAALAAGEAPKYSGRCRSLSPSEVAARRSRGEPAAVRFRVPEGGD
                     VAFLDGVRGLVSFPRATIGDFVLLRSDGNPAYNFAVVVDDALMEVTQVVRGEDHISNT
                     PRQVLLYEALGYRAPRFAHVALVMGPDHTPLSKRHGATSVAEFRARGYLPEALLNYLA
                     LIGWSPGGDQEVLPIDELARRFRLEDVGRSAGVFDEEKLAWVNRHYMRLAGASRLARL
                     AAPFLRAEGIVVTEPDAGGWAYLESVVVMASDSVDRLADIPGRLRFLFSYDAARALAR
                     PEVQAIVNEDGAREVVRCLAHELAASERLTSREAFRAAADRVKRETGRKGKALFHPIR
                     VALTGEAGGPELDHAVPAIERGADLPASSGLAPIAGCGERVRMFLDALDRA"
     gene            complement(3150..>3921)
                     /gene="ispF"
                     /locus_tag="PKK95_14865"
     CDS             complement(3150..>3921)
                     /gene="ispF"
                     /locus_tag="PKK95_14865"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOC19547.1"
                     /translation="PGATGGRPSIARTLPRESIHLAQTPQAFRIEVLEEAVRLGREGI
                     EATDEAALAERAGYAVRLVEGSTRNIKITTPDDLAIAEALVAPKSAEPALRVGMGYDL
                     HRLVEGRRLVLGGVVVPFERGLAGHSDADVVCHALTDALLGAAAAGDIGQHFPDTDPK
                     WAGADSLALLREVTALVAALGYRPVNADLTVIAERPKLGPHREAIAASLAAALGVPAG
                     AVSVKAKTNEGVDATGREEAIAAHAVVLLERRAAASRS"
BASE COUNT          581 a         1488 c         1276 g          576 t
ORIGIN      
        1 tcccgacgtt cacgtgcggt ttcgagcggt cgaacttttc tttcgccatc gcgtgttcct
       61 cgctccgcct tcgcggacgc cgccctcgcg gcgtccgctc cggcgggact catgaactgg
      121 agccgatgac caggattgaa ctggtgacct cgtccttacc aaggacgcgc tctgccaact
      181 gagctacatc ggccaacggg acctaaacgg gcacctcgtg cccggcacca agcacaccag
      241 ccttcgctgc ttcgcagcga ccctggcttg ccatgagcga ggccggctgc ccttcgactc
      301 gcgcgcccgg gcgcgtcgag ggcgaacacg cgctcgctca gggcattcga ctcggctgcc
      361 ggccgggtcg aatggagcgg gagacgggga tcgaacccgc gaccaacagc ttggaaggct
      421 gtgactctac cactgagtta ctcccgcctt cgcgaccttg cgctccgatt cacttgctcg
      481 ggtcgctacg gcgggcaagc ccgctcatgc gaccctgcgc tccgattcac taaacgcctt
      541 ttccgcctgc catgagcgag gccggccctt cgactcgctg cgctcgctca gggcaccatt
      601 cgactccggc ctgccggccg agtcgaatgg tggggagggg aggattcgaa cctccgaagc
      661 cacttgggcg acagatttac agtctgttgc gtttgaccgc tctgcaacct ccccacacgt
      721 cgtcggccgg ccggctcgcc cggcgggcgg acggacccga tggagctggc ggagggattc
      781 gaacccccga cccgctgatt acaaatcagc cgctctaccg gactgagcta cgccagccag
      841 acgaaaggac tcaatctagc acaagctgtg ccaagcaagc aagtttcggt cacttttggc
      901 cagctttttg gccctcacgg gccgtccccg cccgctggcg gagcgcctcg aaaagcacga
      961 ttcccgcagc cacgctcacg ttcaggctgt ccacgtggcc ccgcatggga agcgaggcca
     1021 gcaggtcgca tcgctcccgg acgagccgac gcagcccgct tccttccgcc ccgagaacga
     1081 tggccgaggg ctgcgtgaga tccacctcgc tgtagggcaa cggcgcgtcg cctgccagcc
     1141 ccaccgtcca gacgttcgcc tcgcgcagct cctcgagcgc gcgggcgatg ttcaccacgc
     1201 tcgccacccg gacgtgcgcc agcgccccgg ccgacgcctt cgccgccgcc ccgtcgaggg
     1261 cggccgcgcg ccgcgtctgc ctgatcaccc cgtcggcgcc cgccgcatcc gccaccctca
     1321 ggatcgcccc gaagttgtgc gggtcttcca cgccgtccag gacgaccagc agcgccggcc
     1381 cgaacgcacc ctccaccagc tcggctaccc cgtagtcgcg ggcctcgtca atctcggcca
     1441 cgaccccttg gtggcggccg ccgcgcgcgg cccgatcgag ttcggccggt tcggcacggc
     1501 gtaccgccac tccggccccc gcggccagct cgaacagctg gcgcatgcgg cggtcgtgtc
     1561 gatcggcgac gcggatctcc cgcacgcacc cggcccgcag cgcctcgatc acggggttga
     1621 ggccgtagat gatcacatcc caatccgtca ggcccggtcg agtgcgtcga ggaacatgcg
     1681 cacgcgctcg ccgcagcccg caatcggcgc cagaccagaa gaggccggca gatccgcacc
     1741 gcgttcgatc gccggaacgg cgtggtcgag ttcgggcccg ccggcctcgc cagtcaacgc
     1801 cacccggatc gggtggaaca gcgccttccc cttccgcccg gtctcgcgct tcaccctgtc
     1861 ggcggctgca cgaaacgcct ccctgctcgt gagcctctcc gaagccgcca gttcgtgagc
     1921 caggcacctg acgacctcgc gcgccccgtc ctcgttgaca atggcctgca cctcggggcg
     1981 ggccagcgcc cgggcggcgt cataggagaa caggaagcgg aggcgacccg ggatgtcggc
     2041 aaggcggtcc accgagtcgg aggccatcac caccacgctc tcgaggtatg cccacccccc
     2101 tgcgtccggc tcggtcacga cgatgccctc tgcccgcaag aacggcgcgg cgagcctcgc
     2161 gaggcgggag gcgccggcca gccgcatgta gtgccggttc acccaggcaa gcttctcctc
     2221 gtcgaacacg ccggcgctgc ggcccacgtc ctcgagcctg aagcgccggg ccagctcgtc
     2281 gatcggcaac acctcctggt cgccgccggg cgaccacccg atcagcgcga ggtagttgag
     2341 caacgcctcg ggcaggtagc ctcgcgcgcg gaactccgcc accgaggttg caccgtgacg
     2401 cttggagagc ggggtgtgat cggggcccat gacgagcgcg acgtgcgcga accgcggcgc
     2461 gcggtacccg agggcctcat acagcagtac ctggcggggc gtgttcgaga tgtggtcctc
     2521 gccccgtacc acctgcgtca cctccatcag cgcatcgtcg acaacgaccg cgaagttgta
     2581 cgcggggttc ccgtccgaac gaagcaggac gaagtcgccg atcgtggccc tcgggaacga
     2641 caccagcccc cgcaccccgt cgaggaaagc gacgtcgccg ccctccggaa ccctgaacct
     2701 cacggcggcc ggctcgccgc gcgaccgccg agccgcgacc tcggatggag aaaggctccg
     2761 gcaacggccg ctgtacttcg gagcctcgcc ggcagcgagc gccgcttccc gctctgcctt
     2821 tagttgcgcc tccgggcaga agcagtagta agcgcgccct tcggcgagca gacgatcggc
     2881 ctgcgcgcgg tagatgtcca gccgctccga ctgccggtac ggtccgcggt cgcctcccac
     2941 gcccggcccc tcgtcccact cgaggccgag ccactcgagg tcctcgagaa tcgcctgctc
     3001 ggaggcggac gtcgagcgct cgcggtcggt gtcctcgatc cgcaggatga acgtcccgcc
     3061 gctgccgcgc gcgagcagcc agttgaacag cgcggtgcgc gcgttgccta cgtgcaggtg
     3121 gccggtgggg cttggtgcga agcggactct catgatcgcg atgcggctgc ccgccgttcc
     3181 aggagcacga ccgcgtgcgc cgcgatcgct tcctcacggc ccgtcgcgtc cacgccctcg
     3241 ttggtcttcg ccttcacgct caccgcgcca gccgggacgc ccagcgccgc cgccagcgag
     3301 gcggcgatcg cctcgcggtg gggcccgagc ttcgggcgct cggcgatcac ggtgaggtcg
     3361 gcgttgaccg ggcggtaccc gagcgcggca acgagcgccg tcacctcgcg cagcagcgcg
     3421 aggctgtcgg cgccggccca cttcggatcg gtgtcgggga agtgctgccc gatgtcgccc
     3481 gccgccgcgg cgccgagcag cgcgtcggtg agcgcgtggc agacgacgtc ggcatccgag
     3541 tggcccgcca gtccccgctc gaacgggacg acgaccccgc ccaggacgag cctgcgcccc
     3601 tcgaccagcc ggtggaggtc gtaacccatc ccgacgcgca gcgcgggttc ggcggacttc
     3661 ggcgcgacga gcgcctcggc gatggccaga tcgtcgggcg tggtgatctt gatgttgcgc
     3721 gtgctcccct ccaccagtcg caccgcgtag ccggcgcgct cggcgagcgc cgcttcgtcg
     3781 gtcgcctcga ttccctcgcg ccccagcctg accgcctcct cgagcacctc gatgcggaac
     3841 gcctgcggcg tctgggcgag gtggatcgac tcgcgcggca gggtccgcgc gatcgaaggc
     3901 cttccacccg tcgcgccagg c
//
LOCUS       DAOPQX010000353         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_25195_length_3907_cov_4.286345, whole genome shotgun sequence.
ACCESSION   DAOPQX010000353 DAOPQX010000000
VERSION     DAOPQX010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25195_length_3907_cov_4.286345"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /gene="folE"
                     /locus_tag="PKK95_14870"
     CDS             complement(<1..526)
                     /gene="folE"
                     /locus_tag="PKK95_14870"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004912347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOC19548.1"
                     /translation="MQDLIRTLLKELGEDPEREGLEQTPRRVERAFRFLTGGYKADVD
                     QVLNNALFTVDYSEMVIVKDIDFYSLCEHHLLPFFGRCHVAYIPNGKVIGLSKIPRLV
                     DVFARRLQVQERMTSQIAETIEKKIDPLGVAVVIEARHLCMAMRGVEKQNSVAVTSAM
                     LGAFRNSARTRTEFL"
     gene            complement(552..1352)
                     /locus_tag="PKK95_14875"
     CDS             complement(552..1352)
                     /locus_tag="PKK95_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOC19549.1"
                     /translation="MPSLISRLAMPMTNNSSNVGVPHGPGSGLEAVVLAAGRGSRLFS
                     VETLPKPVYAVEGRPLLARVVDGLQAAGVSTVHVVTGYGAGAVRKCPGVERDGLRVNW
                     IDNPRFDEPNGLSLMCARGAVGPRFLVSMSDHLFQHGAIARFAAGAPADGEYLAVDYD
                     LAGIFDMDDATKVRTRDGNAIEAIGKDLDTFTAVDTGLFMLTPRVFDALEESGRAGDH
                     SLSGGIRRLAARGLMRAWDIGGGKWLDVDTPDASREAARLIRAGLIGG"
     gene            1512..2375
                     /locus_tag="PKK95_14880"
     CDS             1512..2375
                     /locus_tag="PKK95_14880"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOC19550.1"
                     /translation="MAIERVGILGCGLMGSGIAQVCAQAGYATIVREVDQAALDRGLA
                     RIRSFLDEGVAKGKVAADARDQAWARLSGTTRVEELAGCDLVVEAVVEDLEAKRQAYA
                     ALQPVLGPPALLASNTSSLSITELAAASGRPGRFAGLHFFNPVPLMKLVEVVRGLETA
                     DRTWRDLVAFARSLGKEPVTAPDRPGFIVNRLLFPYLLDAVRSHEAGLGTVEEIDAAM
                     KLGCGHPMGPFALLDFIGLDTAYSIATVMYEEFRDPAFAPPPLLKRLVLARRLGRKSG
                     HGFYRYQDGPK"
     gene            2748..3083
                     /locus_tag="PKK95_14885"
     CDS             2748..3083
                     /locus_tag="PKK95_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC19551.1"
                     /translation="MVVNGNSDMLELLESVLDAGHYDIVFVAETEHAYSEIKRARPNL
                     IILCVAIDDPNGFHVLSMLKLDEETAAIPILTYTGEPEGEETDREPDDDSPEDELPLV
                     TRRPELSMN"
     gene            complement(3311..>3907)
                     /locus_tag="PKK95_14890"
     CDS             complement(3311..>3907)
                     /locus_tag="PKK95_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19552.1"
                     /translation="RVFSEAPEDSPSRQLARFVKAVGAGYVPSQQVTLVARHDRFGRG
                     GDHTAFNQQGFAAVRLTEAVENYERQHTAADTLEGVNIPYLMRNVRLNAAVAASLALA
                     PPAPVVTDERGQPTLGRRPSGYDANLRWAPSPGAVGYVVYWREAWTPDWQHAVRVGDV
                     CEHLIRNVSIDDHVFGVSAVGPNGEESVTAAYVNPPRR"
BASE COUNT          596 a         1359 c         1342 g          610 t
ORIGIN      
        1 ccaggaactc ggtgcgggtg cgggcactgt tgcggaaggc gccgagcatc gccgaggtga
       61 cggccaccga gttctgcttc tcgaccccgc gcatcgccat gcacaggtgc ctcgcctcga
      121 tgacgacggc cacgcccaga gggtcgatct tcttctcgat cgtctcggcg atctggctgg
      181 tcatccgctc ctgcacctgc aggcgccggg cgaacacgtc caccagccgc gggatcttac
      241 tgaggccgat caccttcccg ttcgggatgt aggcgacgtg gcagcggccg aagaacggca
      301 gcaggtggtg ctcgcaaaga ctgtagaagt cgatgtcctt gacgatcacc atctcgctgt
      361 agtcgacggt gaacagcgcg ttgttcagca cctggtccac gtcggccttg tagccgccgg
      421 tcaggaaccg gaaggcccgc tcgacgcgcc ggggcgtctg ctcgagcccc tcgcgctcgg
      481 ggtcctcgcc cagctccttc aacagcgtcc gaatcaggtc ctgcatgtgc gtctctctag
      541 ccgttccctt cctagccccc gatcagcccc gcccggatca gccgcgccgc ctcgcgcgag
      601 gcgtccggcg tgtcgacgtc gagccacttc ccgccgccga tgtcccaggc gcgcatcagg
      661 ccgcgcgccg ccaggcggcg gatcccgccc gacagcgagt ggtcgccggc tcgcccgctt
      721 tcctcgagcg cgtcgaacac gcggggcgtg agcatgaaga gccccgtgtc caccgcggtg
      781 aacgtgtcga ggtccttccc gatggcctcg atggcgttgc cgtcccgcgt gcgcaccttg
      841 gtggcgtcgt ccatgtcgaa gatgccggcc aggtcgtagt ccaccgcgag gtactcgccg
      901 tcggcgggcg cgcccgccgc gaagcgggca atcgccccgt gctggaacag gtggtcggac
      961 atcgagacga ggaagcgcgg ccccaccgcc ccgcgcgcgc acatcagcga cagcccgttc
     1021 ggctcgtcga accgcgggtt gtcgatccag ttgacgcgga ggccgtcgcg ttcgaccccg
     1081 gggcacttgc gcacggcgcc ggccccgtag ccggtgacca cgtggaccgt ggagacgccc
     1141 gccgcctgca gcccgtccac cacgcgggcg agcagcggcc gtccttccac cgcgtagaca
     1201 ggcttgggaa gcgtttccac cgagaacagc cgggacccgc gccctgctgc cagaaccacc
     1261 gcctcgagcc cgctgcccgg cccgtgcggc accccgacgt tcgaagagtt gttggtcatc
     1321 ggcatggcaa gccgcgaaat gagcgagggc aacttttcat tcttcaggtc cgggcacggt
     1381 cgtgtcaagg acaggcccgg cacagtggca caggcgtccc gcctgtgaac agtggcacag
     1441 gcgtcccgcc tgtgaacagt ggcacaggcg tcccgcctgt gagctgtgcc acaatgcccc
     1501 ggggggttcc catggcgatc gagcgcgtcg gcatccttgg ctgcggcctg atgggcagcg
     1561 gcatcgcgca ggtttgcgcc caggcgggct atgccaccat cgtccgcgag gtggaccagg
     1621 cggccctcga caggggcctg gcccgcatcc ggtcgttcct ggacgagggc gtggcgaagg
     1681 gaaaggtggc ggctgacgcc cgcgaccagg cgtgggcgcg gctcagcggc acgacgcgcg
     1741 tggaggagct agccggctgc gacctggtgg tcgaggcggt cgtcgaggat ctcgaggcca
     1801 agcggcaggc ctacgccgct ctccaaccgg tgctcgggcc gccggccctc ctggcctcga
     1861 acacctcgtc gctgtcgatt accgagctgg cggccgccag cggccggccc ggccggtttg
     1921 ccggcctgca cttcttcaac cccgtcccgc tgatgaagct ggtggaggtg gtgcggggcc
     1981 tcgagaccgc cgaccggacg tggcgcgacc tggtggcctt cgcccggtcg ctcggcaagg
     2041 agccggtaac cgcccccgac cggcccggct tcatcgtcaa ccggctgctc ttcccctacc
     2101 tgctcgacgc ggtgcggtcg cacgaggcag gtctcggcac cgtcgaggag atcgacgcgg
     2161 cgatgaagct gggctgcggc cacccgatgg ggccgttcgc gctgctcgac ttcatcgggc
     2221 tcgacacggc ctattccatc gccaccgtga tgtacgagga gttccgggac ccggcgttcg
     2281 ccccgccccc gctcttgaag cggcttgtcc tggcgcgccg cctcgggagg aagagcggcc
     2341 acggattcta ccggtaccaa gacgggccga agtgaaccgt tcggcccgcg ttccggcccg
     2401 ctcacggctt ccctggaccc tgtgagtacc gcgatctgcc gccggcctgc ggccgtggtc
     2461 gttgccgaaa acgacacgtt gcgggcacga gacggttcat acctgtaaag gccgttccgc
     2521 caagaggtta tcgtgtcagg ccgcggccgt gagacgacgg ggtgtcagcg cgggccgaca
     2581 gctcatgcac agagacgcgg gatttctaag aaaaatcagg catttgtcgg ctgccctgtt
     2641 ggctccgttc ttgaatagag aacgggcaag gggaacccac tgtgatgctg accagggtga
     2701 atcagatcgg tcgactccga cgacccggtc acgcgaccca gaaggtcgtg gtagtcaacg
     2761 gcaacagcga catgctcgag ctgctcgagt cggtcctcga tgccggccac tacgacatcg
     2821 tcttcgtagc cgagaccgag cacgcgtaca gcgagatcaa gcgggcccgg ccgaacctga
     2881 tcatcctctg cgtcgccatc gacgacccga acggattcca cgtgttgtcg atgctgaagc
     2941 tcgacgagga gacggcggcc atcccgatcc tcacctacac gggcgagccg gagggggagg
     3001 agaccgaccg cgaacccgac gatgacagcc cggaggacga gctgccgctg gtgacccgtc
     3061 gccccgagtt gtcgatgaac tgaggggccg cagcgcaggg ctttagccct gaggcacacg
     3121 ttagcagtgg cacaggcgtc tcgcctgtga cggcatatgg cccgcggcgt tccgcgggtc
     3181 caggcggcta cggggccagg accgggagtc cagtatgtcg gcgccggcag atgtccggcc
     3241 tcccgcgctg gatgggtcct ggtccccgga cgctgggcct gagccttgag ccctgagccc
     3301 tgagccggaa tcacctccgc ggcggattga cgtacgcggc cgtcacgctt tcctctccgt
     3361 tcggtcccac cgcagacacc ccgaacacgt gatcgtcgat tgagacgttc cggatgaggt
     3421 gttcgcagac gtctcccacc cggacggcgt gctgccaatc gggcgtccac gcctcgcgcc
     3481 aatagacgac atatcccacg gcacccggcg acggcgccca tcgcaggttc gcgtcgtagc
     3541 cgctgggccg gcggccgagc gtgggctggc cgcgttcgtc cgtcacgacc ggggcggggg
     3601 gcgcgagggc cagcgaggcg gcgaccgccg cgttcaggcg gacgttccgc atcaggtacg
     3661 ggatgttgac cccctccagc gtgtcggccg ccgtgtgctg ccgctcgtag ttctccacgg
     3721 cctcggtcag gcgcacggcc gcgaacccct gctggttgaa ggcggtgtgg tcgccgccgc
     3781 ggccgaagcg atcgtggcgg gcgaccaggg tcacctgctg ggacgggacg tagccggcgc
     3841 ccaccgcctt gacgaagcgg gccagctggc gcgagggcga gtcttcgggc gcctcggaga
     3901 agacgcg
//
LOCUS       DAOPQX010000354         3888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_25339_length_3888_cov_4.222019, whole genome shotgun sequence.
ACCESSION   DAOPQX010000354 DAOPQX010000000
VERSION     DAOPQX010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25339_length_3888_cov_4.222019"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1164)
                     /locus_tag="PKK95_14895"
     CDS             complement(184..1164)
                     /locus_tag="PKK95_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOC19553.1"
                     /translation="MWSDLGVDLTPALYRLLGDQARLRLLRVLARERLNVTELTAVLG
                     VAQSGVSRHLGLLKEAGLVAEERAGGFAYYRVAPRDGSAAADALWNLLDAHFAIVSAG
                     PAGREDDARLHEVMRLRQENFEDHGGGEGRQLVPGRSWAAWARALGLLMPPLTVADLG
                     CGEGYLTIEVARWARRVIAVDRSAAVLKSARALAARHGLRNITWRRGEIEAVPLPDGR
                     VDLVLLSQALHHASDPALALAEAARVLAPGGKVLVLDLRRHDQHWVRDRLDDRWLGFE
                     DRALGTAMRKAGFAEVRQQVGARRKGDPFTVIVASGTKPGAARAAKSQRP"
     gene            1552..2682
                     /gene="asd"
                     /locus_tag="PKK95_14900"
     CDS             1552..2682
                     /gene="asd"
                     /locus_tag="PKK95_14900"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOC19554.1"
                     /translation="MAPGGRWDVGVLGATGLVGQQIVRRLEGHPWFRTCWLGASERSA
                     GARYGELPWLLSEARPKSVDGLLVERATPGQAPRLLFSALDASAAGEIETAFAKAGHI
                     VVSNARSHRLAPDVPLLVPEVNPSHLALLEAQRRKRRWQGAIVTNPNCSTAFLVMVLA
                     ACREFRPRRVVVTTLQAISGAGYPGLPSWDVMGNVIPFIDGEEEKIQVETAKILGTLD
                     GDTVDDDPIAVSAQATRVPVADGHTEAISIEFDRPPSQAALVEALARFSGRPQQAGLP
                     SAPRSPIEIVSGRDRPQPRLDLARGDGMTVSVGRVRPCPVLHYRLVALGHNTVRGAAG
                     AALLNMELLAADGWLEHPAGAGVRDRQPAVAGRHVTGPGGEA"
     gene            2688..>3888
                     /locus_tag="PKK95_14905"
     CDS             2688..>3888
                     /locus_tag="PKK95_14905"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOC19555.1"
                     /translation="MKFGGSSVATAAAIARVRSIVSREPRPRVIVVSALGGVTDDLLS
                     AAASAETGDLRQALRVIDALGERHRETARAALASIPTTLLPALDHCWFRASATLRAIA
                     RRGRGSARARDEVAACGELASSLLVRHALEDAGLPAEWIDARRAIATDGRHGSAAPLY
                     APTAGRLRSSVLPVLGRGGIPVVGGFVGSTLRGVTTTLGRGGSDLSASLVGACLDAEE
                     IQIWTDVDGLLTADPRVFDRPRPVAALTFGEAGELAHFGAKVLHPAAVDPAANARIPV
                     RILNSIRPGAAGTLIAGHHDARPGLAALASHRGLAALDVDCTPATRERVLVQLFSECA
                     RAMLAVHLAALADSRISIVAGSGAPLDRVAKALGRRAGVRRREGLALLAAVGEGLRER
                     GPGEERAA"
BASE COUNT          523 a         1446 c         1401 g          518 t
ORIGIN      
        1 ctgcccggcg aacccttcga aacccatccc ctgccgctcg taccttccgg ccaccggtcg
       61 gagtgatccg cccgcctcca gtggcacagg cgtccggcct gtcagcgcca ccgcctccag
      121 tggcacaggc gtctcgcctg tgtcagcggc accacttcca gtggcacggg cgtctcgcct
      181 gtgtcaggga cgttgggact tggctgcccg cgcggctccc ggcttcgtcc cgctcgccac
      241 gatcaccgtg aacggatcgc ccttgcgccg ggcgccgacc tgctggcgca cctccgcgaa
      301 tcccgccttg cgcatcgcgg tgccgagcgc ccgatcctcg aagcccagcc agcgatcgtc
      361 cagccggtcg cgcacccagt gctggtcgtg gcgccggagg tcgaggacga gcaccttgcc
      421 gcctggcgcc agcacgcggg ccgcctccgc gagcgccagc gccgggtccg aggcgtggtg
      481 cagcgcctgc gagagcagca ccaggtcgac gcggccgtcc ggcagcggaa ccgcctcgat
      541 ctcgccgcgc cgccaggtga tgttccgcag gccgtggcgc gcggcgagcg cgcgggccga
      601 cttcaggacg gcggccgacc ggtccacggc aatcacgcgg cgggcccacc gcgccacctc
      661 gatggtgaga tagccctcgc cgcaccccag gtctgcaacg gtcaatggcg gcatcagcag
      721 gccgagtgcg cgcgcccacg cggcccagct gcgcccgggc accagctgcc gcccctcacc
      781 gccgccgtgg tcctcgaaat tctcctggcg cagccgcatc acctcgtgca gccgcgcatc
      841 gtcttcacgc ccggccggcc ccgccgagac gatggcgaag tgggcgtcga ggaggttcca
      901 gagcgcatcg gctgccgcgc tgccgtcgcg cggcgccacg cggtagtagg cgaacccgcc
      961 cgcgcgctcc tcggccacca gccctgcctc cttgagcagc ccgaggtgcc gcgagacgcc
     1021 cgactgcgcg acgcccagca cggcggtcag ctcggtgacg ttgagccgct cgcgggcgag
     1081 caccctcagg aggcggaggc gggcctggtc tcccaggagg cgatagaggg ccggcgtcag
     1141 atccacgccc agatctgacc acatcgctcc cggccgcgca aacccggtgc ggacgccggc
     1201 cgttctcagt gagcgtcccg cgcagtggca cacgcgcagt ggcacaggcg tcccgcctgt
     1261 ggggggcgcc ccccggaggg gcggcacgcg tcctcggaca cgtaacatac atcaattgac
     1321 atacgttgat atttcttctt gacaccccgc agagcccggc cctagaatcc atcccagagt
     1381 gttaaccctt ctcttcccca ccgggcacct gatggcgatg cgaagccaca acatcggtgg
     1441 gggagccgtg cccggaccgt agagaccggg cgcgaaaggc caaggccagg caaacccgcc
     1501 gattggcagc agccggtcgg cgggtttttt cgcgtacgag gggagggacc agtggcaccg
     1561 ggcgggcgat gggacgtggg cgtgctgggg gcgaccggcc tcgtggggca gcagatcgtg
     1621 cggcgcctgg aggggcatcc ctggttcagg acgtgctggc tcggggcgag cgaacggtcg
     1681 gccggggccc ggtacggcga gcttccctgg ctgttgagcg aggcgcggcc gaagagcgtc
     1741 gacggcctcc tcgtcgagcg cgcgactccc gggcaggcgc cccgcctgct cttctccgcg
     1801 ctcgacgcgt cggccgccgg agaaatcgaa acggcgttcg ccaaggcggg gcacatcgtc
     1861 gtcagcaacg cccggtcgca ccgcctggcc ccggacgtcc cgctgctcgt ccccgaagtg
     1921 aaccccagcc acctggccct cctcgaggcg cagcgccgga agcggcgatg gcagggcgcc
     1981 atcgtcacga accccaactg ctcgaccgcc ttcctcgtca tggtgctcgc tgcctgccgc
     2041 gagtttcgcc ctcggcgggt cgtcgtgacc acgctgcagg cgatctcggg cgccgggtac
     2101 ccgggcctgc cgtcgtggga cgtcatgggc aacgtcatcc cgttcatcga cggcgaagag
     2161 gagaagatcc aggtcgagac cgcgaagatc ctcgggacgc tggacggcga cacggtcgac
     2221 gacgatccca tcgcggtgag cgcccaggcg acgcgggtcc cggtcgccga cggccacacc
     2281 gaggcgatca gcatcgagtt cgaccggccg ccgtcgcagg ccgcgctcgt cgaggcgctc
     2341 gcccgcttct cgggccggcc gcagcaggcg gggctgccgt cggcacccag gtccccgatc
     2401 gagatcgtgt ccgggcgcga ccggccgcaa ccgaggctcg acctggcgcg aggcgacggg
     2461 atgacggtgt cggtcgggcg cgtccgccca tgcccggtgc tgcactaccg gctggtcgcc
     2521 ctcggccaca acaccgttcg cggcgcggcg ggcgcggccc tcctcaacat ggaactgctg
     2581 gcagcggacg gctggctgga gcacccggcc ggcgccggcg tgcgcgaccg gcagccggcg
     2641 gtcgcgggca gacacgtgac aggcccggga ggtgaggcgt gatcgtcatg aagttcggcg
     2701 gatcgtcggt ggcgacggcc gcggcgatcg cgagggtgag gtcgattgtc tcgagggaac
     2761 caaggccgcg ggtgatcgtg gtgtcggccc tcggcggcgt gaccgacgac ctgctctctg
     2821 cggcggcaag cgcggagacg ggagatctgc gccaggccct gcgcgtgatc gacgcgctgg
     2881 gcgagcggca ccgcgagacg gcccgcgccg ccctggcgtc catccccaca acgcttctcc
     2941 cggcactgga ccactgctgg tttcgggcct ccgcgacgct gcgagcgatt gcgcggcggg
     3001 gccgtgggtc ggcccgcgcg cgcgacgagg tcgctgcctg cggagagctc gccagcagcc
     3061 tgctcgtgag acatgcgctc gaggacgccg gcctgcctgc ggagtggatc gacgcccggc
     3121 gggcgatcgc caccgatggc cggcacggct ccgccgctcc actctacgcg ccaaccgccg
     3181 gccggctgcg ctcgtccgtc ctgccggtgc tcggacgagg aggcatcccg gtcgtcggcg
     3241 gcttcgtcgg atccaccctc cgcggcgtca ccacgacgct cggccggggc gggtcggacc
     3301 tctcagcgtc gctggtcggc gcctgcctcg atgcggagga gatacagatc tggaccgacg
     3361 tcgacggcct gctgaccgcc gacccacggg tcttcgaccg gccgcgcccg gtcgccgcgc
     3421 tcaccttcgg cgaggcgggc gagctggccc acttcggcgc gaaggtgctt cacccggcgg
     3481 ccgtggaccc ggcggcaaac gccaggatac ccgttcgcat cctgaactcg atccgccccg
     3541 gcgcggcagg caccctgatc gcggggcatc acgatgcccg tcccgggctt gccgccctgg
     3601 ccagccaccg cggtctcgcg gcgctcgacg tggactgcac ccccgccacg cgcgagcggg
     3661 ttctcgtgca gctgttcagc gagtgcgcgc gggcgatgct cgccgtgcac ctggccgccc
     3721 tggcggactc gcggatctcg atcgtggccg gctcgggtgc gccgctcgac agggtggcga
     3781 aggcgctcgg ccggcgcgcg ggcgtccgcc gccgcgaagg acttgccctg ctggccgccg
     3841 tgggcgaggg cctgagagag aggggcccgg gcgaggaacg cgcggcgg
//
LOCUS       DAOPQX010000355         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_25447_length_3875_cov_4.645812, whole genome shotgun sequence.
ACCESSION   DAOPQX010000355 DAOPQX010000000
VERSION     DAOPQX010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25447_length_3875_cov_4.645812"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..851)
                     /locus_tag="PKK95_14910"
     CDS             complement(36..851)
                     /locus_tag="PKK95_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOC19556.1"
                     /translation="MRARGCRTVRRQLSAYRDGELPVSEQIAVEAHVRACRACADAVA
                     ELDAIGALLRRSVPESGESLAGLAATVVSRVKAEREQSLRGWTERVFEDMHFVWAGLG
                     AAGATLACVAIIFGIFYYATHERPDSLGGVLATLSLPAGSNGNPLQADTRILLPSAAN
                     GDAFPEAVLPDEDESVFALAASLTREGRIANLELLHAEATGGNLRSAQREKEILTLLD
                     SISKARFEPARYGNSPVAVNVVWLYTQLTVRGKAPGSVPPQSRSALRAAGLSA"
     gene            complement(856..1455)
                     /locus_tag="PKK95_14915"
     CDS             complement(856..1455)
                     /locus_tag="PKK95_14915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOC19557.1"
                     /translation="MKRAPAIGWSDVGGREATLISRCAAGDEVACTELVNEHQRMVYQ
                     LALHLLGDPEEALDLSQEVFLNVFRTIGRFRGQSALRTWIYRIVVNQVRNRQRWWKRR
                     RRSDQVSLEQRLEAQGDLPSPHVLEEPDRMFGRKELASRIGAALKGLPFEQRTAIILR
                     EIDGLSYEEIAYSLGVAVGTVKSRLTRAREALRAQLREA"
     gene            1620..2996
                     /locus_tag="PKK95_14920"
     CDS             1620..2996
                     /locus_tag="PKK95_14920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19558.1"
                     /translation="MRRTVVAALLLALAATAAVLIWQDAGRKAAFRRLVEAGEAALAA
                     GNTFPALEAFSGAIALQEHSMIAHLRRGETYRRRGEAGQALRDLRTAVRLDPNATRPL
                     ELIGDINAELARFARAAEAYEGYLRLDDQSPRVLYKLALVRVRLGQPAAALPPLRQAL
                     ELDDRFVEAAYLLGVCERTLGRPAEAAAALEWAVRLSPDLIAAREELAELYGAARRDT
                     DALAQLEALAALEPDRAERRIAIGLAHARAGRRDQAVLTLRKLAEEEPANPAVFVAIA
                     RVWLDAAESGGDRIILGKALEALEAVARRGTPSPEALTLLGRAHLLSGDNAAAERSFR
                     QATSRLPVDPAALLQLSLAAERAGHLATARDALVRYTTLSSDGLAGPERALHLGELSM
                     RLNEPEAAAAWFAHAAQAPGAGPTAFMRLAQAELRLGRRQAARAAVERGLRHDPGHAG
                     LLQLAATL"
     gene            complement(3030..>3875)
                     /locus_tag="PKK95_14925"
     CDS             complement(3030..>3875)
                     /locus_tag="PKK95_14925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOC19559.1"
                     /translation="VLVEGESGTGKELVARALHQRGPRRHRRLCALNCAALNDELFEA
                     ELFGHSRGAFTGAVAERKGLFEEADGGTLVLDEVGELTPRAQAKLLRAIQEGEIRRVG
                     ENFARAVDVRLVASTNRPLRAAVEEGRFRRDLLYRLDVVRIVVPPLRDRVEDIPTLAA
                     HFWQVATSRLGSRATLSPGAITALARYDWPGNIRELQNVIAALAVSAGQRGSVGPGSL
                     PGAIAGAPARAATTLEQARTIFEARYVRAALARSGGRRGEAARELGVTRQGLAKLLAR
                     LSIEP"
BASE COUNT          535 a         1463 c         1334 g          543 t
ORIGIN      
        1 cgccgaagtg aaggagtgag atcgcgggcg cccggctagg ccgagaggcc ggcggcgcgc
       61 aaggccgaac gagactgcgg aggcacgctt cccggggcct tgccccgcac cgtcagctgg
      121 gtgtagagcc acaccacgtt gacggccacc ggtgagttgc cgtaccgcgc gggttcgaag
      181 cgcgccttcg agatgctgtc gagcagcgtc aggatttcct tctcgcgctg ggcgctgcgg
      241 aggttgcccc cagtggcctc ggcgtgcagc agctcgaggt tggcaatccg gccctcccgc
      301 gtgagcgatg ccgcaagcgc gaagaccgac tcgtcctcgt cgggcagcac cgcctcgggg
      361 aaggcgtcgc cgttggccgc gctcggcagc aggatgcggg tgtcggcctg cagcgggttc
      421 ccgttcgacc cggcgggcag cgacagggtc gccagtacgc ctccgagcga gtcgggacgc
      481 tcgtgcgttg cgtagtagaa gatcccgaag atgatggcca cgcaggccaa agtcgcaccg
      541 gccgccccga gcccggccca gacgaagtgc atgtcttcga aaacgcgctc ggtccagccg
      601 cgaagcgact gctcgcgctc ggccttcacc cggctcacga cggtggccgc gagcccggcc
      661 agcgactcgc cgctctccgg gacgctgcgt cgcaggagtg cgccaatcgc atccagctcg
      721 gccaccgcgt cggcacaggc ccggcacgcg cgcacgtgag cctcgacggc gatctgttcc
      781 gagacgggca gttcaccgtc tctgtaggcc gacaactgac gacgcaccgt ccggcaccct
      841 cgggccctca tggcctcacg cctccctcag ctgggcccgc aacgcctcgc gggccctcgt
      901 cagccgcgac ttcaccgtcc cgaccgcgac cccgagcgag tacgcaattt cctcgtagct
      961 cagcccgtcg atctcgcgca ggatgatcgc cgtgcgctgc tcgaacggca gccccttcaa
     1021 cgccgcgccg atgcgcgacg cgagctcctt ccggccgaac atccggtcgg gctcctcgag
     1081 cacgtgcggc gagggcaggt cgccctgcgc ctcgagccgc tgctcgagcg acacctgatc
     1141 cgaccggcgg cggcgcttcc accaccgctg gcggttgcgg acctggttga cgacgatgcg
     1201 atagatccag gttcggaggg cggactggcc gcggaagcgg ccgatcgtac ggaagacgtt
     1261 gaggaagacc tcttgggaga ggtcgagtgc ctcctccggg tccccgagga ggtgcagggc
     1321 gagctggtag accatccgct ggtgctcgtt caccagctcg gtgcaggcga cctcgtcgcc
     1381 agccgcgcaa cggctgatca acgtcgcctc gcggcccccg acatctgacc agccaatggc
     1441 tggcgctcgc ttcaccgtca gttccccgga caccgtggga cgctcctcac tatagcaggt
     1501 tgcgctcgat cgtgtcagga acagttgggc ttcgcggcgc ccagtccaca cctcgtcccc
     1561 ttcacctcgt tagacccgaa cccggccgga aagttccgct gtgctgctac cataggcgca
     1621 tgaggcggac cgtggtcgcg gcgcttctgc tcgccctggc agccaccgcg gccgtgctga
     1681 tctggcagga cgcgggccgc aaggccgcct tccgccggct cgtcgaagcg ggggaagcgg
     1741 ctttggccgc cggcaacacg ttccccgccc tcgaggcgtt cagcggcgcc atcgccctgc
     1801 aggagcactc gatgatcgcc cacctccggc ggggcgaaac ctaccgccgg cggggcgaag
     1861 ccggacaggc gctgcgcgac ctccggacgg ccgtcaggct cgacccgaac gcgacccggc
     1921 cgctcgagct gatcggcgac atcaacgccg agctggcgcg attcgcacgc gccgccgaag
     1981 cctacgaggg ctacctgcgg ctcgacgacc agtcgccgcg cgttctctac aagcttgcgc
     2041 tcgtgcgggt gcgcctgggc cagccggctg cggccttgcc gccgctgcgg caggccctcg
     2101 agctcgacga ccgcttcgtc gaggccgcct acctcctcgg cgtctgcgag cgcaccctcg
     2161 gccggcctgc cgaggcggcc gccgccctcg aatgggccgt gagactgtcg ccggacctga
     2221 tcgccgcccg agaggagctt gcggagctgt acggcgccgc tcgccgcgac accgacgcgc
     2281 tggcccagct cgaagcgctt gccgcgctcg aacccgaccg cgccgagcgg cgcatcgcga
     2341 tcggcctggc gcatgcccgg gccggccggc gcgaccaggc cgtgctgacc ctccgcaagc
     2401 tcgcggagga ggagcccgcg aaccctgccg tgttcgtcgc gatcgcccgc gtgtggctcg
     2461 acgccgccga gtcgggaggc gaccgcatca tcctcggcaa ggcgctcgag gcgctggagg
     2521 cggtcgcgcg gcgagggact ccatcgcccg aggcgctgac gctgctcggc cgcgctcacc
     2581 tgctctcggg tgacaacgcc gcggccgaac ggagtttccg ccaggcgacc tcgcggctcc
     2641 cggtcgatcc ggcggcgctc ctgcaactgt cgctcgcggc cgagcgggcc ggccatcttg
     2701 ccaccgcccg cgacgcgctg gtgcgctaca cgaccttgtc gtccgacggc ctggccggcc
     2761 cggaacgcgc gctgcacctc ggcgagctgt cgatgcggct gaacgagccc gaggcggcgg
     2821 cggcctggtt cgcccacgcg gcccaggccc cgggtgcggg gccgacggcg ttcatgcggc
     2881 tggcccaggc cgaacttcgg ctcggccgcc gccaagcggc gcgggccgcg gtggagcgag
     2941 ggctccggca cgaccccggg cacgctggtc tgctccagct cgcggccacc ctctgacgcg
     3001 gaccggcccg cctcgactca cggcttcctt caaggctcga tcgataggcg cgccagcagc
     3061 ttggcgagcc cttgcctggt caccccgagc tcgcgcgccg cttcgccgcg ccggcctccg
     3121 cttcgagcga gggcggcccg aacgtagcgc gcctcaaaga tggttcgggc ctgctcgagc
     3181 gtcgtggccg cgcgggccgg ggcgccagcg atcgcgcctg gcaggcttcc cggtcccaca
     3241 cttccccgct gcccggccga gacggcgagg gcggcaatca cgttctgcag ctcgcggatg
     3301 ttgccgggcc agtcgtagcg cgccagggcc gtgatcgccc cgggcgacag ggtcgcccgg
     3361 ctcccgaggc ggctggtggc cacctgccag aagtgcgcgg cgagcgtcgg gatgtcctcg
     3421 acgcgatcgc gcagcggcgg cacgacgatc cggacgacat cgagtcggta cagaaggtct
     3481 cggcgaaagc gcccttcctc gaccgccgcc ctcaatggtc ggttggtgga ggcaaccagc
     3541 cgcacgtcga cggcacgcgc gaagttctcg cctacccgcc ggatctcgcc ctcctgaatg
     3601 gcgcgcagca gcttcgcctg cgcgcgaggc gtcagctcgc ccacctcgtc gagcacgagc
     3661 gtcccgccgt cggcctcttc gaacagcccc ttccgttcgg ccacggcgcc ggtgaaagcg
     3721 cctcgcgagt ggccgaacag ctcggcctcg aacaactcgt cgttcaaggc ggcgcagttg
     3781 agcgcgcaca acctccggtg gcggcgcggc ccgcgctggt gcagcgccct cgccaccagc
     3841 tccttgccgg tgccgctctc gccctcgacg agcac
//
LOCUS       DAOPQX010000356         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_25628_length_3856_cov_3.757432, whole genome shotgun sequence.
ACCESSION   DAOPQX010000356 DAOPQX010000000
VERSION     DAOPQX010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25628_length_3856_cov_3.757432"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="PKK95_14930"
     CDS             <1..993
                     /locus_tag="PKK95_14930"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOC19560.1"
                     /translation="IRKELEEKKKRVRQISALLKDESSRWKVVREEILAVQQKYGSAK
                     VDRRRSIIESVGEEVEYTADDFIQDEDNIVLVSRDGWVKRQREVRDLSTTRLREGDVV
                     LAALAGSTRASVVFFTNFGVAYTARVIDIPATTGYGEPIQRFFKFRDGEKVMAAFSLD
                     PRAIGEIEADEGEVPKVHALAVTSDGFSFRFGLGAFVEPSTRAGRRFARPAEGAEVVG
                     VSVVDGRETVIAVTEQARAMLCKVEEINFLSGPGKGVILIKIAPGQDRVLGFIASKGD
                     RDLLTVETNRGAAQTVSTAKYEVTGRGGRGRELLQRGQFRRIIPPPAELPGALG"
     gene            1028..1957
                     /locus_tag="PKK95_14935"
     CDS             1028..1957
                     /locus_tag="PKK95_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOC19561.1"
                     /translation="MGTAGVSWDVLGVGANSVDLVHVLPAFPEPEGWRSKMRIERRLV
                     HCGGQAATTTAACARLGLRAKYVGVTGDDEHGARVRAALAARGIGVDGLVVREGAANQ
                     FAVILIDEKSGERAVLWERDPALRLRPGEMPWEAMEAARLLHVDDVDQDAAIALALRA
                     RARGVMVTSDLDRADGRAEELVAAVTVPVFTDGLPQALTGLDDREAALRKLRRPHHRL
                     LCVTLGQAGALALEGDRPILSSGLHVDAVDTTGAGDIFRGGLILGLLGGWPIERTLAF
                     ANATAALSCTRHGALDSVPSLGEVEALLAECRM"
     gene            1991..3523
                     /locus_tag="PKK95_14940"
     CDS             1991..3523
                     /locus_tag="PKK95_14940"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOC19562.1"
                     /translation="MNPSRYLVRDIPRRRFLELGVKAGAALAATPLLLDTLLAAPSPA
                     PPPVAVDKAVLDRVLARALARGGTFADVYLENRLSRNILLEESRFKSAEVGISQGAGV
                     RVIVGDKTGYAYADEVTEEKMLRAAEVASFIARGGQPAQPASISEQRRPSYVTVRRPL
                     NEVADERRLDVMRRASDAALKHDPRIRMASVSYYDEVRGRVVATSEGAYLADELPLLF
                     FIVQTLSEGNGTRHMGRERLSRHSGFEMFEEVTPEEVARAAARESIAMLEAKDAPAGR
                     MDVVMQNGWGGVLVHEAVGHPLEADNIARQIGAFTGKLGQTVASPVFTMVDDGTIPNA
                     RGTTNFDDEGTPMQRNVLIKDGVLLKYMTDILSAGQLKAERTGNGRRESFRYLPIPRM
                     TNTYIAAGTDKPADILASTPSGLYVQSLSGGSVDPTTGVFNFTCREAYLIENGKKTTP
                     VKGATLIGSCLDIIRNIDAVGDDLAFGPGICGKGQSAEVTAGQPTVRLRGITVGGTRA
                     RK"
     gene            3546..>3856
                     /locus_tag="PKK95_14945"
     CDS             3546..>3856
                     /locus_tag="PKK95_14945"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HOC19563.1"
                     /translation="MDYKGLTGQLVKTCLGCGADAAEVYLEAGRRLGIDVRNGALETV
                     EEAASQGVGVRVFVKGRMAFCSSNDLRPEALESAAARAIEFARSTTADASNVLPSETE
                     MS"
BASE COUNT          604 a         1274 c         1466 g          512 t
ORIGIN      
        1 atccgcaagg aactggagga gaagaagaag cgcgtgcgcc agatctcggc tctcctgaaa
       61 gacgagtcga gccgctggaa ggtggtacgg gaggagatcc tcgcggtcca gcagaagtac
      121 ggctccgcca aggtggaccg ccgccgctcg atcatcgagt cggtgggcga ggaggtcgag
      181 tacaccgccg acgacttcat ccaggacgag gacaacatcg tcctcgtgtc tcgggacggg
      241 tgggtgaagc ggcagcgcga ggtgcgcgac ctgtcgacca cccggctgcg cgagggcgac
      301 gtggtgctgg ccgcgctggc ggggagcacc cgcgcgagcg tcgtgttctt caccaacttc
      361 ggcgtcgcgt acaccgcccg ggtgatcgac atccccgcga cgaccggcta cggcgagccg
      421 atccagcggt tcttcaagtt ccgggacggc gagaaggtga tggcggcctt cagcctcgac
      481 ccgcgagcca tcggcgagat cgaggcagac gagggagagg tgccgaaggt gcacgcgctg
      541 gcggtcacca gcgacggctt cagcttccgc ttcggccttg gagcgttcgt cgagccgagc
      601 acgcgggcgg gccgccggtt cgcacggccg gccgagggcg ccgaggtggt gggcgtgtcg
      661 gtggtcgacg ggcgcgagac ggtgattgcc gtcaccgagc aggcgcgggc catgctgtgc
      721 aaggtcgagg agatcaactt cctctccggg cccggcaagg gagtcatcct catcaagatc
      781 gccccgggcc aggaccgggt tctcggcttc atcgcctcga agggggaccg cgacctgctg
      841 acggtcgaga ccaatcgggg cgcggcgcag acggtgtcca cggcgaagta cgaggtcacc
      901 gggcggggcg gacgcggccg cgaactgctg cagcgcggcc agttcaggcg gatcatcccg
      961 ccgccggcgg agctgccggg agcgctgggg tagggcaggc tggtgcaggc ataacggcgg
     1021 aggcgacgtg ggtactgcgg gtgtgagctg ggacgtcctg ggggttggcg ccaattcggt
     1081 ggacctcgtc cacgtgctgc ccgcgttccc cgagccggag ggctggcgct cgaagatgcg
     1141 gatcgagcgg cgcctcgtcc actgcggcgg gcaggccgcc accaccacgg ccgcctgtgc
     1201 gcggcttggc ctgcgggcga agtacgttgg cgtcacgggc gacgacgagc acggggcgcg
     1261 cgtgcgcgcg gccctggccg cgcgcggcat cggcgtggac ggcctggtcg tccgggaagg
     1321 cgcggcgaac cagttcgcgg tcatcctcat cgacgagaag agcggggagc gggccgtgct
     1381 gtgggagcgg gaccccgcgc tgcgcctccg tcccggcgag atgccctggg aggcgatgga
     1441 ggcggcccgc ctgctacacg tggacgacgt ggaccaggac gcggcgatcg cgctggcgct
     1501 ccgcgcgcgg gcccggggcg tgatggtcac gagcgacctc gatcgggccg acgggcgcgc
     1561 cgaagagctg gtggccgcgg tcaccgtgcc ggtgttcacc gacggcctgc cgcaggcgct
     1621 gacgggcctc gacgaccgcg aggcggcgct gcggaagctg cgccgcccgc accaccggtt
     1681 gctgtgcgtg acgctggggc aagccggggc gttggccctc gagggcgacc gcccgatcct
     1741 ctcctccggc ctgcacgtgg acgcggtgga tacgaccggg gccggcgaca tcttccgggg
     1801 agggctgatc ctcggcctgc tcggggggtg gccgatcgag cggacgctcg ccttcgccaa
     1861 cgcgaccgcc gccttgtcgt gcacgcgcca cggcgccctc gacagcgtcc cctcgctcgg
     1921 cgaggtggag gcgctgctcg cggagtgccg aatgtagcgg tatgcgcccc ctttctcccc
     1981 agaggccgtc atgaatccct ctcgctacct ggttcgcgac atcccccgcc gtcggttcct
     2041 ggaactgggc gtcaaggcag gcgccgccct cgcggcgacg ccgctgctgc tcgacacgct
     2101 gctggccgcg ccgtccccgg cccccccgcc ggtggcagtt gacaaggcgg tgctcgaccg
     2161 cgtgctggcg agggcgttgg cgcggggcgg gacgttcgca gacgtctacc tggagaaccg
     2221 cctgtcgcgc aacatcctgc tcgaggaatc gcgcttcaag agcgccgagg tgggtatcag
     2281 ccagggggcc ggcgtccggg tcatcgtcgg cgacaagacc ggctacgcct acgccgacga
     2341 ggtcaccgag gagaagatgc tgcgggcggc cgaggtcgcc tcgttcatcg cgcgaggcgg
     2401 acagccggcg cagccggcga gcatctccga gcagcgccgg ccgtcctacg taacggtccg
     2461 gcggccgctg aacgaggtgg ccgacgagcg gcggctcgac gtgatgcggc gagccagcga
     2521 cgcggcgctg aagcacgatc cgcgcatcag gatggcctcg gtcagctact acgacgaggt
     2581 ccgcggccgc gtcgtggcca ccagcgaggg ggcctacctc gccgacgagc tgcccctcct
     2641 cttcttcatc gtgcagaccc tgagcgaagg gaacggcacc cgccacatgg ggcgcgagcg
     2701 cctcagccgc cactccggct tcgagatgtt cgaggaggtc acgcccgagg aggtggcgcg
     2761 cgcggcggcc cgcgagtcga tcgcgatgct cgaggcgaag gacgccccgg ccggcaggat
     2821 ggacgtcgtg atgcagaacg ggtggggcgg cgtgctggtc cacgaggcgg tggggcaccc
     2881 cctcgaggcc gacaacatcg cccggcagat cggcgcgttc acgggaaagc tgggacagac
     2941 ggtcgcgagc cccgtcttca cgatggtcga cgacgggacg atcccgaacg cgcgcgggac
     3001 caccaacttc gacgacgagg ggacgccgat gcagcgcaac gtcctcatca aggacggcgt
     3061 gctgctcaag tacatgaccg acatcctctc ggccggccag ctgaaggccg agcggaccgg
     3121 gaacgggcgc cgcgagagct tccgttacct gccgatcccg cgaatgacca acacgtacat
     3181 cgcggcaggg acggacaagc cagccgacat cctcgcgtcc acgccgagcg ggttgtacgt
     3241 gcaaagcctg tcgggcggca gtgtggaccc gaccaccggg gtgttcaact tcacctgccg
     3301 cgaggcctac ctgatcgaga acgggaagaa gacgacgccg gtgaaggggg ccacgctgat
     3361 cgggagctgc ctcgacatca tccggaacat cgacgcggtg ggcgacgacc tggcgttcgg
     3421 tcccggcatc tgcgggaagg ggcagtcggc cgaggtaacc gcggggcagc ccaccgtccg
     3481 cctgcgcggc atcaccgtgg gcgggacgcg ggccaggaag taggcgagcg cgggccggag
     3541 aacctatgga ctacaaaggg ctcacagggc aactggtgaa gacgtgcctg ggttgcggcg
     3601 ccgacgcggc cgaggtgtac ctcgaagcgg ggcgccggct gggcatcgac gtccggaacg
     3661 gggcgctcga aaccgtggag gaggccgcct cgcagggagt cggcgtgcgc gtgttcgtga
     3721 agggacgcat ggcgttctgc agctcgaacg acctgcggcc cgaggcgctc gagtcggccg
     3781 ccgcccgcgc gatcgagttc gcgaggtcca ccaccgccga cgcgagcaac gtgctgcctt
     3841 ccgagacgga gatgtc
//
LOCUS       DAOPQX010000357         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_25800_length_3838_cov_3.781919, whole genome shotgun sequence.
ACCESSION   DAOPQX010000357 DAOPQX010000000
VERSION     DAOPQX010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25800_length_3838_cov_3.781919"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PKK95_14950"
     CDS             complement(<1..988)
                     /locus_tag="PKK95_14950"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOC19564.1"
                     /translation="MLALLCLLLQVQQPALAESGPPAAPAIQGSGSPSVPIPRIDAAV
                     TIDGRLDEEAWASAARLDGFWQYRPVDGRPAEERTEVLVWYSPTALHLGVIAHDREPG
                     TIRATLADRDNLTRDDTVTFFLDTFSDRRRAFFFTVNPLGAQEDGVQTEGAFNAGTMY
                     GGTADKNPDYQWDSRGRLTDTGYVVEIRIPFKSLRYQGSGAQRWGFNVQRKVQRTGYE
                     DTWTDVRRASSSFLAQAGTIEGLHDLKRGVVTEVQPFVTTANNGAAGENGLFERGSTD
                     FNPGANFRLGLTNLSFDATVNPDFSQVESDASQVTANERFALYYAEKRPFFLE"
     gene            1200..3005
                     /locus_tag="PKK95_14955"
     CDS             1200..3005
                     /locus_tag="PKK95_14955"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOC19565.1"
                     /translation="MKRAALILSCLLGLSVAIPAQQAVPRPEYPRPQFERADWLNLNG
                     PWEFEFDDRNVGLDEGWAAGERQFGRTIVVPFCFESRASGIGDVSFHPWVWYRRTVTV
                     PPAWKGRRVLLNFGAVDYRAMVWVNGRLAGAHEGGNTPFGFDVTAFLKEGENVITVRA
                     EDPPTDRSIPRGKQYWQVQSSGIFYTRTTGIWQTVWLEAAGTSYLERPRVRTSLDGTV
                     RMDLRVARPVDNLELRATVKLKDQVVASGTGPVVAGRASVAVAVREPRLWSPGSPNLY
                     DVVYELVSGGRVIDRVSSYFGIRSVGVRQGRVTLNGDATYLRMMLDQGYWPESLLTPP
                     SDEAIQYDIRMTKEMGFNGARKHQKVEDPRFIYWADRMGLLVSGEMANAYVYDEDYAA
                     RFTREWIEAVERDINSPSVVVWAPINESWGTPDLSDPRQQAHLKSLYQLTHSLDGTRP
                     VIDNEGWEHTDQTDLFAIHDYARSGDILFEKYQDVGKPGADVPDNALPALAPGYQYNG
                     SPVYLSEFGGIAFIPPGHQVPKQAWGYSGVEKTPEAALERMRGLYEAIARLKFAGLCY
                     TQLTDVEQEVNGLMTYDRKPKFDLKTVREIVTLAR"
     gene            3082..>3838
                     /locus_tag="PKK95_14960"
     CDS             3082..>3838
                     /locus_tag="PKK95_14960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOC19566.1"
                     /translation="MADPFQPPLRSECPEDTAPQDADEPVDEEPEPEEPPRAREGLPP
                     GYRMRADAHYVEQFVTRRVGEPLELIPVGRIDGPHPTSGNLEPLVRSIARHGILQPLI
                     VRRHEGRYQLIAGSRRLAAAMAAGLEAVPCLIRTGDDTTCRDLAEAANIGHMPAPQAP
                     APQPLPEGSPLVQAGYPELMEHLGAISACLHLFGERERPVRERVAFGLIRAEVNRAAW
                     LAQALAVLTGSPSPSRVPLDLRSLVRRVAASMEP"
BASE COUNT          583 a         1292 c         1367 g          596 t
ORIGIN      
        1 cctcgaggaa gaagggcctc ttctcggcgt agtacaaggc aaaccgctcg ttcgcggtca
       61 cctggctggc gtccgactcg acctggctga agtcggggtt caccgtggcg tcgaacgaca
      121 ggttggtgag accgagccgg aagttggcgc ccgggttgaa atcggtggag ccgcgctcga
      181 agagcccgtt ctccccggca gccccgttgt tggccgtcgt gacgaacggc tgcacctcgg
      241 tgaccacgcc gcgcttcagg tcgtggagcc cttcgatggt gcccgcctgc gcgaggaagc
      301 tcgagctggc gcgccggacg tcggtccagg tgtcctcgta tccggtgcgc tgcaccttgc
      361 gctgcacgtt gaacccccag cgctgcgcgc cgctcccctg gtaccgcagg ctcttgaacg
      421 ggatccggat ctcgacgacg tagccggtgt cggtgagacg gcccctcgag tcccactggt
      481 agtcggggtt cttgtcggcc gtccccccat acatcgtccc cgcgttgaag gccccctcgg
      541 tttggacgcc gtcctcctgc gcgcccagcg ggttgaccgt gaagaagaac gcgcggcggc
      601 gatcgctgaa ggtgtcgagg aagaaggtga cggtgtcgtc gcgcgtcagg ttgtcgcggt
      661 cggcgagcgt cgcgcggatc gtgccgggct cgcggtcgtg cgcgatcacc ccgaggtgga
      721 gcgcggtcgg cgagtaccag acgagcacct cggttcgttc ctcggccggc ctgccgtcca
      781 ccgggcggta ctgccagaac ccgtcgaggc gcgcggccga tgcccacgcc tcctcgtcga
      841 ggcggccgtc gatcgtcacg gcggcgtcga tccgcgggat gggcaccgag ggggagccgc
      901 tgccctggat ggccggggcg gctggcggac cactttcggc gagtgcgggt tgctggacct
      961 ggagcaacag gcagagcagt gcgagcacgt gtggttccga gagggaaagc cgctacggca
     1021 gggccgctgt cggcggtact cgtcgcgggg gcgggccggg gaccggcacg attctatctc
     1081 accccggtgg accgcgtggt cccgaatcct gaatcctgaa tcccgactcc tgatgggaat
     1141 tgggattggc cgttcaacct gatagcatcc ggaagttctt tcttcccgga ggagcccacg
     1201 tgaaacgagc cgctttgatt ctgtcgtgcc tgctcggcct gtcggtcgcg atccctgcgc
     1261 agcaggcggt gccgcgcccg gagtacccgc gcccccagtt cgagcgggcc gactggctca
     1321 acctgaacgg cccgtgggaa ttcgagttcg acgaccgcaa cgtcggcctc gacgagggat
     1381 gggcggccgg cgaaaggcaa ttcggccgca ccatcgtcgt tcccttctgc ttcgagagcc
     1441 gcgcgagcgg catcggcgac gtgtcgttcc atccgtgggt ctggtaccgg cgcacggtga
     1501 cggtgccgcc cgcgtggaag ggccggcgcg tgctgctcaa cttcggcgcg gtggattacc
     1561 gcgcgatggt ctgggtgaac ggccggcttg ccggcgcgca cgagggcggc aacaccccgt
     1621 tcgggttcga cgtcacggcc ttcctgaagg agggtgagaa cgtcatcacc gtccgtgcgg
     1681 aggatccgcc gaccgatcgg tcgatcccca gggggaagca gtactggcag gtgcaatcgt
     1741 cggggatctt ctacacgcgc acgaccggca tctggcagac cgtctggctc gaggcggcgg
     1801 ggacgagcta cctcgagcgg ccgcgcgtgc ggacctcgct cgacggcacc gtccggatgg
     1861 acctgcgcgt ggcgaggccg gttgacaacc tcgaactgcg ggcgacggtg aagctgaagg
     1921 accaggtggt cgcctccggc accgggcctg tggtggccgg gcgcgcgtcg gtggcggtgg
     1981 ccgtgcggga gccccgcctg tggtcgcccg gctcgcccaa cctgtacgac gtggtgtacg
     2041 agctggtgtc gggcggccgg gtcatcgacc gcgtgtcgtc ctacttcggc atccggagcg
     2101 tgggcgtgcg gcaggggcgc gtgacgctga acggagatgc cacctacctg cggatgatgc
     2161 tcgaccaggg gtactggccc gaatcgctgc tgacgccgcc gagcgacgag gcgatccaat
     2221 acgacatccg gatgacgaag gaaatggggt tcaacggcgc acgcaagcac cagaaggtgg
     2281 aggacccgcg gttcatctac tgggccgacc ggatgggact gctggtgtcg ggcgagatgg
     2341 ccaacgcgta cgtctacgac gaggactacg cggcccggtt cacgcgcgag tggatcgagg
     2401 ccgtcgaacg cgacatcaac agcccctcgg tcgtcgtctg ggcgccgatc aacgagagct
     2461 gggggacccc cgacctgtcc gaccccaggc agcaggccca cctgaagtcg ctctaccagc
     2521 tgacgcactc gctcgacggc acgcggccgg tgatcgacaa cgaggggtgg gagcacaccg
     2581 accagaccga cctgttcgcg atccacgact acgcgaggag cggcgacatc ctgttcgaga
     2641 agtaccagga cgtgggcaag cccggcgcgg acgtgcccga caacgcgctg ccggcgctgg
     2701 cgcccggcta ccagtacaac ggctcgccgg tctacctctc cgagttcggc ggcattgcct
     2761 tcattccacc cggccaccag gtgccgaagc aggcgtgggg atactcgggc gtggagaaga
     2821 cccccgaggc ggccctcgag cggatgcgcg gcctgtacga ggccatcgcc cgcctgaaat
     2881 tcgccgggct gtgctacacg cagctcaccg acgtcgagca ggaggtcaac ggcctgatga
     2941 cgtacgaccg caagccgaag ttcgacctga agacggtgcg ggagatcgtc acgctcgcgc
     3001 gatgagccgc ggggcacgcc gcatggccct tgttctcgcg taacggttca gctattctcg
     3061 acgctcgtgt cgcacatgcc gatggcagat cccttccagc ccccgcttcg cagcgaatgc
     3121 cccgaggaca ctgctcccca ggacgccgac gagccggttg acgaggaacc tgagcccgaa
     3181 gagccgccgc gcgcgcgcga gggcctgccc cccggctacc gcatgcgggc cgatgcgcac
     3241 tacgtcgaac agttcgtcac gcggcgggtc ggcgagccgc tcgagctgat cccggtcggg
     3301 cgcatcgacg ggccgcaccc cacctcggga aacctcgagc cgctggtccg ctcgatcgcg
     3361 cgtcacggca tcctgcagcc gctcatcgtg cgccggcacg agggacgcta ccagctgatt
     3421 gccggctccc gccggctcgc ggccgccatg gcggcggggc tggaggcggt tccctgcctg
     3481 atccggaccg gcgacgatac gacctgtcgc gacctggccg aggcggcaaa catcgggcac
     3541 atgccggcgc cacaagcgcc cgccccgcag ccgctcccag agggatcgcc gctggttcag
     3601 gccggctatc ccgagttgat ggagcacctg ggggcgatca gcgcctgcct gcacctgttc
     3661 ggtgaacgcg agcggccggt gcgcgagcgg gtcgccttcg gcctgatccg ggccgaagtc
     3721 aaccgggccg cgtggctggc ccaggccctc gcggttctca ccgggtcgcc gtcgccttcg
     3781 cgggtgccgc tcgacctgcg atcgctcgtg cgccgggttg ccgcgtcgat ggagccgg
//
LOCUS       DAOPQX010000358         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_25896_length_3828_cov_3.939041, whole genome shotgun sequence.
ACCESSION   DAOPQX010000358 DAOPQX010000000
VERSION     DAOPQX010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25896_length_3828_cov_3.939041"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PKK95_14965"
     CDS             <1..1029
                     /locus_tag="PKK95_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19567.1"
                     /translation="TPGPSATVRPDGGLADPKDKLAAFTAVQEAGALIADGDHEQAAQ
                     RLEAAIRGEPDMPQALLMLGSCYTELGRRKEARSKFDQVLRKDPQSVQALIGMANVLL
                     EDGQADDVVALCRRTLSIDPRNTQAYTLLGDVYIGRHEPSKALPYLEKAVEIQPKITQ
                     NRLNLAACLVEVRQLPRAQALLQEILAAQPGFPGAQFNLGVLYEELQRPEEARAAYLA
                     EIGNNPNAFKARFNLGKMQAASGDWTGSAEQMREVVRIAPRRPEGYLFLARARLQQSA
                     PIDEVEVLADQGLARASTPELKALGWFVKADVYGRRGRPDRVKAALGNAQAELARQPR
                     AAGAEPRH"
     gene            1076..1954
                     /locus_tag="PKK95_14970"
     CDS             1076..1954
                     /locus_tag="PKK95_14970"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOC19568.1"
                     /translation="MDRAVHGMPSTVSRVASSVVVVILIGAVASAAGQGRPPFQSPVR
                     AEIVASPPASKNGAAWLGIRLVMNEGWHVYWTNPGDSGGPPAVHWELPAGLSAGPLGW
                     PLPERIPFDRMVNFGYYGEVVLPARLSADGGRWPGGPFTVNAQVRWVACRDICVPGRA
                     ALSKDLPAGFATSRPDGDLLRRAVAQVPVPAPRAWRLSGTSAGDTFTVALETGRGEKA
                     VTFFPLLPGQVEAAAPVEATPTPRGASLTLRKSAYLSKPIASLKGVIVVGGRGYEVDV
                     PLRPVPSNPAEGHRQH"
     gene            complement(2037..>3828)
                     /locus_tag="PKK95_14975"
     CDS             complement(2037..>3828)
                     /locus_tag="PKK95_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOC19569.1"
                     /translation="VSMTLSLAAVFIPVFFMGGVVGRLMHEFAVTIGAAILVSGLVSL
                     TLTPMLCSRFLKAPHAIRHGRLYQATERMFDAWLRGYSWSLRLAIRFKGTTMVLSALL
                     LAGTVYLFTVMPMGFIPAVDTGQLSGQIETIQGLGFDATVARVKDVMNILANDPNVAS
                     YTANAFGSGGRLNVDLVPRERRTLTADQVIAQLRPKFAQIPGIRVFLTNPPAIRIGGM
                     MTRSQYQFTLQDPDTHELYAVAPNFEAALRRLEGIEDVSSDLQLSNPQIDVELDRDRI
                     AALGLTVDQVELALNSAYGSRQVSQIFAPDDQYQVIMQVAPRYQRDPAALSLLYVQAP
                     QNHLVPLSAVVSARQTAGPLSVNHTGQLPSVTLSFNLAPGVSLGDAVARVQAVARETL
                     PDTVAGSFQGTAQAFQESMTGLGWVLALAIFVIYVVLGILYESFIHPFTILSGLPAAG
                     FGALLTLLLFRHDLNIYAFVGVIMLVGLVKKNGIMMVDFAVERRRLGLSAADAIYDAC
                     RVRFRPIMMTTMSALMGTLPIALGWGAGSEARRPLGLAVVGGLLVSQTLTLYVTPVFY
                     VYMERLQDWLARRKTGREPVLAEAGIAAPE"
BASE COUNT          620 a         1341 c         1353 g          514 t
ORIGIN      
        1 acccctggtc cctccgcgac ggtccgcccg gacggcgggc tggccgaccc caaggacaag
       61 ctcgcggcgt tcacggccgt gcaggaggcc ggggccctga ttgccgacgg cgatcacgag
      121 caggccgcgc agcggctcga ggccgcgatc cggggcgagc ccgacatgcc ccaggccctg
      181 ctgatgctcg gcagctgcta caccgagctc ggacggcgga aggaggcccg gtcgaagttc
      241 gaccaggtgc tccggaagga tccgcagagc gtgcaggcgc tgatcggcat ggccaacgtc
      301 ctgctggagg acgggcaggc cgacgacgtg gtggcactgt gcaggcggac gctctccatc
      361 gacccgcgca acacgcaggc gtacacgctc ctcggcgacg tctacatcgg gcggcacgag
      421 ccgtcgaagg cgctgccgta cctcgagaag gccgtcgaga tccagcccaa gatcacccag
      481 aaccggctga acctggctgc ctgcctggtc gaggtccggc agctgccccg cgcgcaggca
      541 ctgctgcagg aaatcctggc cgcccagccc gggttcccgg gagcgcagtt caacctgggc
      601 gtgctgtacg aggaactgca gcggccggag gaggcccgtg ccgcgtacct cgcggaaatc
      661 ggcaacaacc ccaacgcctt caaggccagg ttcaacctgg gaaagatgca ggcggcgtcg
      721 ggcgactgga cggggtctgc cgagcagatg cgggaagtcg tccgcatcgc gcccaggcgg
      781 ccggaaggat acctgttcct ggcccgcgcg cgactgcagc agtcggcacc catcgacgag
      841 gtggaagtgc ttgccgacca gggcctggcc cgggcgagca cccccgagct gaaggcgctg
      901 gggtggttcg tgaaggccga tgtttacggg cgccgcggcc ggcccgaccg ggtcaaggcc
      961 gccctgggca acgcccaggc ggagcttgca cggcagccgc gcgcggccgg cgcagagccc
     1021 aggcactgaa gccgcggggt cgcatgcccg tcgaggaccg gaggagattt cgcagatgga
     1081 tcgagccgtg cacggcatgc cgagcaccgt gagccgcgtg gcgtcctctg tcgtcgtggt
     1141 cattctcatc ggcgccgtgg catcggctgc cgggcaggga aggccgccgt tccagtcgcc
     1201 cgtccgcgcc gagatcgtgg cgtcaccgcc cgcttcgaag aacggcgcgg cgtggctcgg
     1261 gatccgcctc gtgatgaacg agggctggca cgtgtactgg accaaccccg gggattcggg
     1321 cggcccgccc gcggtgcact gggaactgcc cgcggggctg tcagccggtc cgctcgggtg
     1381 gccgctgccc gagcgcattc ccttcgaccg gatggtcaac ttcgggtact acggcgaagt
     1441 cgtcctgcca gcccgcctga gcgcggacgg cgggcgctgg ccgggcggac ccttcaccgt
     1501 gaacgcccag gtgagatggg tcgcgtgcag ggacatctgc gtgccggggc gggccgcgct
     1561 ctcgaaggac ctgccggcgg gcttcgccac ctcgaggccg gacggcgacc tcctccggcg
     1621 cgctgtcgcg caggttcccg ttcccgcacc gcgagcgtgg aggctgagcg gaacgagtgc
     1681 cggcgacacc ttcacggtcg cactcgagac gggcaggggc gagaaggccg tcacgttctt
     1741 cccgctgctc cccgggcagg tggaggcggc cgccccggtg gaggcgacgc ccaccccccg
     1801 cggcgcgtcg ctcacgctca ggaagtcggc gtacctgtcg aaaccgatcg cgtcgttgaa
     1861 gggcgtgatc gtggtcggtg gccgcggcta cgaagtggac gtgccgctgc gcccggtgcc
     1921 ctccaacccc gcggaggggc accgccaaca ctagtccaca accaggagcc cccgatgaag
     1981 aagctgatcg tctcgtgcct ggccaggggc gtgaccgagc aactcgggac tcgcccctac
     2041 tccggcgcgg cgatccccgc ctccgccagc acgggttcgc gccccgtctt ccgccgcgcc
     2101 agccagtcct gcagccgctc catgtagacg tagaagacgg gcgtgacgta gagcgtcagc
     2161 gtctgcgaca ccaggaggcc gccgaccacc gcgagcccga gcgggcgccg cgcctccgac
     2221 ccggcccccc acccgagcgc gatcggcagc gtccccatca gcgccgacat cgtggtcatc
     2281 atgatcgggc ggaagcgcac ccggcaggcg tcgtagatcg cgtcggccgc gctcagcccg
     2341 agccgccgcc gctcgacggc gaagtcgacc atcatgatgc cgttcttctt caccaggccc
     2401 accagcatga tgacgccgac gaacgcgtag atgttgaggt cgtggcggaa cagcagcagc
     2461 gtgagcagcg ccccgaagcc ggccgccggg aggcccgaga ggatcgtgaa cgggtggatg
     2521 aagctctcgt agagaatgcc gagcaccacg tagatcacga agatcgcgag cgcgagcacc
     2581 cagccgaggc cggtcatcga ctcctggaag gcctgcgccg tcccctggaa gctgcccgcc
     2641 acggtgtcgg ggagcgtctc acgcgccacc gcctgcacgc gggccaccgc gtcgcccagc
     2701 gacaccccgg gcgccaggtt gaacgacagg gtgaccgagg gaagctgccc ggtgtggttc
     2761 accgagagcg ggcctgcggt ctggcgggcc gacaccaccg ccgagagcgg gaccaggtgg
     2821 ttctgaggcg cctgcacgta gagcagcgac agcgccgcgg ggtcgcgctg gtaccgcggc
     2881 gccacctgca tgatcacctg gtactgatcg tcgggcgcga agatctgcga cacctggcgc
     2941 gacccgtaag cggagttgag cgccagctcc acctggtcga cggtcagccc cagggcggct
     3001 atccggtcgc gatccagctc cacgtcaatc tgcggattgc tgagctggag gtcgctgctc
     3061 acgtcctcga ttccctcgag ccggcgcagg gccgcctcga agttcggcgc cacggcgtac
     3121 agctcgtggg tatcgggatc ctgcagcgtg aactggtact ggctgcgcgt catcatgccg
     3181 ccgatccgga tggctggcgg gttggtgagg aacacgcgga tgcctgggat ctgggcgaac
     3241 ttcggccgca gttgcgcgat gacctgatcg gcggtgagcg tgcgccgctc gcgcggcacc
     3301 agatcgacgt tcagccggcc gcccgacccg aacgcgttcg ccgtgtagct ggcgacgttc
     3361 ggatcgttcg ccaggatgtt catcacgtcc ttgacccgcg ccacggtggc gtcgaagccg
     3421 aggccctgga tcgtctcgat ctgcccgctc agctgccccg tgtccaccgc ggggatgaac
     3481 cccatcggca tcaccgtgaa caggtacacc gtgccggcca gcagcagcgc cgacagcacc
     3541 atcgtcgtgc ccttgaagcg gatggccagc cgcagcgacc acgagtagcc ccgaagccac
     3601 gcatcgaaca tccgctcggt ggcctggtag aggcggccgt ggcggatcgc gtgcggcgcc
     3661 ttgaggaacc ggctgcagag catcggcgtc agcgtgagcg acaccaggcc cgacacgagg
     3721 atcgcggcgc cgatggtgac cgcgaactcg tgcatcagcc ggcccaccac gccgcccatg
     3781 aagaacaccg ggatgaacac ggccgccagc gacagcgtca tcgagacg
//
LOCUS       DAOPQX010000359         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_25989_length_3817_cov_4.520734, whole genome shotgun sequence.
ACCESSION   DAOPQX010000359 DAOPQX010000000
VERSION     DAOPQX010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25989_length_3817_cov_4.520734"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PKK95_14980"
     CDS             complement(<1..450)
                     /locus_tag="PKK95_14980"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOC19570.1"
                     /translation="MGSLVLLQRQIVACTRCPRLRAYCERVAREKKRAHRDEIYWGRP
                     VPGFGDPGARLLIVGLAPAAHGANRTGRMFTGDSSGDFLARALHRAGFASQPTSTRAD
                     DGLRLVGAFLSASARCAPPDNKPLPRELEACFPWLRAEAEALPHVRVI"
     gene            complement(521..1813)
                     /locus_tag="PKK95_14985"
     CDS             complement(521..1813)
                     /locus_tag="PKK95_14985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOC19571.1"
                     /translation="MASRVVMPKLSDTMEEGKILRWLKKEGDKVETGQTLAEVETDKA
                     TVEMEAYSNGTIRKLIAEEGATVPIGTLIAVIGSPDEDISALLEQAEGAKAAAPAAAP
                     PREAEARPAAAARPSAPQARRPAQRPRPSPAPAPAPAPEAPPAEGGRGLRASPLALRM
                     AADAGIDIGSLQGSGPQGRIIRRDIEAAIEQAKAPAPSAPAAAVQVGEVQEIELSSMR
                     RTIAKRLVQSKAPVPHFYLTVDVRMDRAWEAYKALRDENYPVSLNDVIIKATASALRR
                     HPEVNASFAGDRVKQFTRVHIGMAVAIEEGLITPVIRDADSKSLLDIAEEARELAERA
                     RSRRLQPHEYTGSTFSISNLGMMGIEEFSAIINPPEAAILAVGAVREVPVAEGGEVKI
                     GRRMKLTLSVDHRVADGAQGARFLQTLTRMLENPLLIG"
     gene            complement(1948..2928)
                     /locus_tag="PKK95_14990"
     CDS             complement(1948..2928)
                     /locus_tag="PKK95_14990"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOC19572.1"
                     /translation="MALITYRDALNQALREEMHRDPSVFLMGEEVGVYQGAYKVSRGL
                     LQEFGERRVIDTPITEEAFAGAGVGAAMVGLRPVVEMMTWNFSMLAMDQIINGAAKML
                     YMSGGQFTIPMVIRGPGGAAHQLAAQHSQSLESWYAHVPGLKVVAPSTPYDAKGLLKS
                     AIRDNDPVIFFEGETLYGSKGEVPEGEYTIPLGVSDVKREGSDVTIIAWSKMVNVALK
                     ASAALEEDGVSCEVIDPRTLRPLDVEPILESVRKTNRCVIVEEGWEYSGVGAQLAYLV
                     HHEAFDDLDAPVSRVTGADVPMPYAKNLEHLAMPQPGRVIEAVRQVLYLD"
     gene            complement(2969..>3817)
                     /locus_tag="PKK95_14995"
     CDS             complement(2969..>3817)
                     /locus_tag="PKK95_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC19573.1"
                     /translation="GAISTLQPDDYIFASYRDHVHALAKGSDPGRLMAELFGRETGVS
                     RGKGGSMHLFDAGCNMLGGHAIVGGHVPLATGVAFAMKYQQKPQVVLCFFGEAAVNIG
                     AFHESLNMAALWKLPCVYIVENNRYGMGTAIERASAIYDVAQRACAYDMASEAVDGMD
                     VLAVREVVGRAVDLARRESLPTLIEARCYRFMGHSMSDPVHGHYRTKEEVEEQKLKDP
                     IRRYFEVLKEAGDLDQDGFERMDAEVKAIVDRAVEFADNSPEPPAEALYEDVYAEPYG
                     PYTRSR"
BASE COUNT          517 a         1389 c         1318 g          593 t
ORIGIN      
        1 gatcacgcgg acgtgcggca gcgcctcggc ctccgcccgc agccagggaa agcaggcctc
       61 cagctcgcgc gggagcggct tgttgtcggg cggcgcgcac cgcgcggacg cgctgaggaa
      121 ggcgccaacc aggcgcaggc cgtcgtcggc ccgcgtcgaa gtgggctggc tggcgaagcc
      181 ggcgcggtga agcgccctcg cgaggaagtc gcccgagctg tcgccggtga acatgcggcc
      241 ggtccggttc gccccgtgcg cggccggggc caggccgacg atgagcaggc gcgcgccggg
      301 gtcgccaaac cccgggacgg ggcggcccca gtagatctcg tcgcggtggg cgcgcttctt
      361 ctccctggcg acgcgctcgc agtacgcgcg cagccgggga cagcgggtgc aggcgacgat
      421 ctgtcgctgc aggaggacga gggaacccac actctgccgg ctcacggctc caggctcacg
      481 gctcaggaca tcgcggcggc tcagacccgg agccttgagc ctacccgatc agcagcgggt
      541 tctccagcat ccgcgtcagc gtctggagga agcgggcgcc ctgcgcgccg tcggcgaccc
      601 ggtgatcgac agacagcgtc agcttcatgc gccggccgat cttcacctcg ccgccctcgg
      661 ccaccggcac ctcgcgcacg gcgccgacgg cgaggatggc cgcctcgggc gggttgatga
      721 tcgccgagaa ctcctcgatc cccatcatgc cgaggttcga gatcgagaag gtgctgcccg
      781 tgtactcgtg cggctgcagg cggcggctgc gggcgcgctc ggccagctcg cgggcctcct
      841 ccgcgatgtc gagcagcgac ttgctgtcgg cgtcgcgaat gaccggggtg atcaggcctt
      901 cctcgatcgc caccgccatc ccgatgtgca cccgggtgaa ctgcttgacg cggtcgccgg
      961 cgaacgaggc gttgacctcg gggtggcggc gcagcgcgct cgccgtcgcc ttgatgatca
     1021 cgtcgttgag cgagacgggg tagttctcgt cgcggagggc cttgtacgcc tcccaggcgc
     1081 gatccatccg cacgtccacg gtcaggtaga agtgcggcac cggcgccttg ctttgcacga
     1141 gccgtttcgc gatcgtccgc cgcatcgacg acagctcgat ttcctgcacc tcgcccacct
     1201 gcaccgcggc cgccggggcc gacggtgcgg gcgccttcgc ctgctcgatg gcggcctcga
     1261 tgtcgcgccg gatgatccgc ccctgcgggc cgcttccctg cagcgagccg atgtcgatgc
     1321 cggcgtcggc cgccatgcgc agcgcgagcg gcgaggcgcg aaggccgcgg ccgccctcgg
     1381 cgggcggcgc ctcgggcgcc ggggcaggcg cgggcgccgg cgagggacgg ggcctctgcg
     1441 cggggcgccg cgcctgggga gcggacggcc gcgcggctgc agcggggcgc gcctcggcct
     1501 cacgcggcgg cgccgccgcg ggggcggcgg ccttcgcgcc ttccgcctgc tcgagcaacg
     1561 ccgagatgtc ctcgtcgggc gacccgatca ccgcgatcag cgtgccgatc gggaccgtcg
     1621 ctccctcctc cgcgatcagc ttccggatcg tgccgttgga gtaggcctcc atctcgacgg
     1681 tggccttgtc ggtctcgacc tcggcaagcg tctggcccgt ctcgaccttg tccccttctt
     1741 tcttgagcca ccggaggatc ttgccctcct ccatcgtgtc gctcagcttc ggcatgacca
     1801 cgcgggaagc catacgtgct ccttcacaag cagcggacag cggacagcgg aacgtggcgc
     1861 agggcttcag ccccgcggaa accagcgtgc gcgacgacga cgtcccgcag ggccaaagcc
     1921 ctgcgctacg cctctcggcc tctaacccta gtccaggtac aggacctgcc gcaccgcctc
     1981 gatgacccgg cccggctgcg gcatcgccag gtgctcgagg ttcttcgcgt acggcatcgg
     2041 cacgtcggcg ccggtcaccc ggctcaccgg tgcgtccagg tcgtcgaacg cctcgtggtg
     2101 gaccaggtag gcgagctgcg cgccgacgcc cgagtactcc cacccctcct cgacgatgac
     2161 gcagcggttg gtcttccgga ccgactcgag gatgggctcg acgtcgaggg gccgcagcgt
     2221 gcgcgggtcg atcacctcgc acgagacgcc gtcctcctcg agggcggccg acgccttcag
     2281 ggcgacgttc accatcttcg accaggcgat gatcgtgacg tccgacccct cgcgcttcac
     2341 gtcggagacc cccagcggga tcgtgtactc cccctcgggc acctcgccct tcgagccgta
     2401 gagcgtctcg ccctcgaaga agatcaccgg gtcgttgtcg cggatggcgc tcttcagcag
     2461 ccccttcgcg tcgtacgggg tggacggcgc gacgaccttg aggccgggga cgtgggcgta
     2521 ccagctctcg agcgactgcg agtgctgcgc ggccagctgg tgggcggcgc cgcccggccc
     2581 gcggatgacc atcgggatgg tgaactgccc gcccgacatg tagagcatct tggcggcgcc
     2641 gttgatgatc tgatccatcg ccagcatcga gaagttccac gtcatcatct cgaccaccgg
     2701 ccggaggccc accatcgcgg cgcccacgcc ggctccggcg aacgcctcct ccgtgatcgg
     2761 agtgtcgatc acgcggcgtt cgccgaattc ctgcagcagg ccgcggctca ccttgtaggc
     2821 gccctggtag accccgacct cctcgcccat gaggaagacg ctggggtcgc ggtgcatctc
     2881 ctcgcgcagt gcctggttca acgcatcccg gtaagtgatc agggccatag gtcgtcttct
     2941 cgtcgggggc gcgcgggccg cccgggtctc atcggcttcg ggtgtagggc ccgtacggct
     3001 cggcgtacac gtcctcgtac aacgcctcgg ccggcggctc ggggctgttg tcggcgaact
     3061 ccacggcgcg gtccacgatc gccttgacct cggcgtccat ccgctcgaag ccgtcctggt
     3121 cgaggtcgcc cgcctccttc agcacctcga agtaccggcg gatggggtcc ttgagcttct
     3181 gctcctcgac ctcctccttc gtccggtagt ggccgtgcac cgggtcggac atcgagtggc
     3241 ccatgaagcg gtagcagcgc gcctcgatca gggtcggcag gctctcgcgg cgggcgagat
     3301 ccaccgcccg cccgacgacc tcgcggacgg cgagcacgtc catcccgtcc accgcctcgc
     3361 tcgccatgtc gtacgcgcag gcccgctgcg ccacgtcgta gatcgccgac gcccgctcga
     3421 tggccgttcc catcccgtag cggttgttct cgacgatgta gacgcagggg agcttccaga
     3481 gggccgccat gttgagcgac tcgtggaacg cgccgatgtt cacggcggcc tcgccgaaga
     3541 agcacagcac gacctgcggc ttctgctggt acttcatcgc gaaggcgacc ccggtggcca
     3601 gcggcacgtg gccgccgacg atggcgtgcc ccccgagcat gttgcacccg gcgtcgaaga
     3661 ggtgcatcga accgcccttg ccgcgcgaca cgcccgtctc gcgcccgaac agctcggcca
     3721 tcagccggcc gggatcgctc cccttggcca gcgcgtgcac gtggtcgcgg tacgacgcga
     3781 agatgtagtc gtcgggctgg agggtcgaga tcgcccc
//
LOCUS       DAOPQX010000360         3816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26002_length_3816_cov_3.366126, whole genome shotgun sequence.
ACCESSION   DAOPQX010000360 DAOPQX010000000
VERSION     DAOPQX010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26002_length_3816_cov_3.366126"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PKK95_15000"
     CDS             <1..743
                     /locus_tag="PKK95_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19574.1"
                     /translation="DEPQYPPPGAIIDYYLAEGLAVGDASIRLEILDAGGRTIRTFTP
                     GPPAASAQAPGLAQAARRRGAAAVRLSTAPGLHRIVWDLRHGAEAGDARGGVGPLAVP
                     GKYTVRFTAGEMVETKAFELRLDPRVVADGVTQADLEEQLALLVRIQEVSARARQAAA
                     RAHDGLERVPAGNPAGAAVLREILTQLVTAGGPYPQPMLLDQLAGLGRMAGSADMKVG
                     RSAFEYLEELNARLAVLEADLAGALGKN"
     gene            785..2128
                     /locus_tag="PKK95_15005"
     CDS             785..2128
                     /locus_tag="PKK95_15005"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOC19575.1"
                     /translation="MAIARRRLSPAQLLTVSVVALIVTGTVLLALPAAGGRGRVALID
                     AAFTSTSAVCVTGLNVLDTPRDLSLFGQFVLMALIQLGGLGYMTLTTVVAVAIGRQLT
                     VKERLTLHEALNTETMEGLGRFALSVLKLTLAFELTGAALLALRWLHDLGAARAAYYG
                     LFHAVSAFNNAGFALFSDNLVRFRGDWLVNLVVCGLIVCGGLGFVVLRELGHARGLRR
                     LSVHTRLVIGLTALLIVGATAALFFLEGRNPATLAQLPLHERLLASFFQAVTPRTAGF
                     NTIDIGAMAPASLFLLMILMFIGASPGGTGGGVKVTTFGITVAALVATVRGRADTTLF
                     RRRVAPEIVTRAFFISLIAFLALNTIAGLLLVFEGRQLLPTLFETTSAFGTVGLSMGE
                     GGPLSLAGHFSAPGKLLLMAMMLAGRVGPLTLAVALAGGGTPPRVRYPEGRVLIG"
     gene            2140..2823
                     /locus_tag="PKK95_15010"
     CDS             2140..2823
                     /locus_tag="PKK95_15010"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOC19576.1"
                     /translation="MVRRKKQFAVIGLGRLGSAMAATLADLGHDVIGVDANEEHVREL
                     ADVLTQAVQLDATDERALRAAGIQDVDVAVVSIGENIESSLLVVMQLKELGIPTIVAK
                     AVTSLHGRILERLGVSRVIFPERDMAIRVAHSLVLTDVIDYIELSRDFSIVEIPAPPP
                     FVGRTLKELELRPRLGLTLIAIKRRPAPGGPEVTTVSPGAAERIQQHDVLVLLGDNAR
                     MAKLNELLG"
     gene            complement(2838..>3816)
                     /locus_tag="PKK95_15015"
     CDS             complement(2838..>3816)
                     /locus_tag="PKK95_15015"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOC19577.1"
                     /translation="VCYKTPMHKRRWLIGALLVAVGIAVALVFAARSQAYGPYWVDAK
                     AAVSWENPLCGELDAFRGLVKAREVKQRTDEILHASRFLEEDPSSGLYLYETPAGKFW
                     MPAADDLWSLSVVLAEQEHDMYGRPGRLGVRSGDVVVDAGAHVGLFARTALAAGAGKV
                     ITFEVTPRSNAALRRNLAKEIAEGRVVVVEKGVWHEEATLPLVVVEKCSVCNSVSHPW
                     MKATVDVPLTTLDRALAELKVERVDFIKLDIENAEANAIRGARGTIARHHPRLAVALE
                     NSKTRLAYAHEVLGLLQEIHAGYRYSCGAVTNPEKSHRVLPEVLHFYAR"
BASE COUNT          522 a         1342 c         1354 g          598 t
ORIGIN      
        1 tggacgagcc gcagtacccg ccgcccggcg cgatcatcga ttactacctg gccgagggtc
       61 tcgccgtggg agacgcgtcc atccgcctcg agattctcga cgcgggcggg cggacgattc
      121 gaaccttcac ccccgggcca ccggcggcct cggcacaggc gccggggctc gcgcaggcgg
      181 cccgacggcg cggcgcggcc gcggtgcgcc tgtccacggc gcccgggctg caccggatcg
      241 tctgggatct ccgccatggc gccgaagctg gcgatgcgcg tggcggtgtc ggcccgctgg
      301 cggtaccggg caagtacacg gtccgcttca ccgcgggcga gatggtcgag accaaggcct
      361 tcgagctacg gctcgacccg cgggtcgtgg cggacggcgt gacgcaggcc gatctcgagg
      421 aacagctcgc cctgctcgtg cggatccagg aggtctcggc gcgggcgcgc caggcggcgg
      481 cccgcgcgca cgacggcctg gaacgggtgc cggcgggcaa cccggcgggt gcggctgtgc
      541 tgcgcgagat cctgacgcag ctggtcaccg ccggagggcc gtatccgcag ccgatgctgc
      601 tcgatcagct cgcgggcctc gggcgaatgg ccggcagcgc cgacatgaag gtgggacgga
      661 gcgccttcga gtacctcgag gagctgaacg cccgcctggc ggtgctcgag gccgacctgg
      721 ccggagcact tggcaagaac tgaaaatggg gcctggcccc attttcactt tctgcaacgc
      781 tggtatggcg attgcgcgca ggcgcctgtc cccggcgcag ctcctgacgg tcagcgtcgt
      841 cgcgctgatt gttaccggca ccgtgctgct cgccctcccc gccgcgggcg gccgcgggcg
      901 ggtcgccctc atcgacgccg ctttcacctc cacctcggcc gtctgcgtca ccggcctcaa
      961 cgtcctcgac acgccgcgcg acctgtcgct cttcggtcag ttcgtcctga tggcgctcat
     1021 ccagctgggc ggcctcggct acatgaccct caccaccgtc gtcgcggtgg cgataggccg
     1081 ccagctgacg gtgaaggagc gcctgacgct ccacgaggcc ctcaacacgg agacgatgga
     1141 agggctgggc cgcttcgcgc tgtcggtcct caagctgacg ctggccttcg agcttaccgg
     1201 tgcggccctg ctcgcgctcc ggtggctgca cgacctcggc gccgcgcggg cggcgtacta
     1261 cggcctgttt cacgcggtct ccgcgttcaa caacgccggc ttcgccctct tctccgacaa
     1321 cctggtccgg ttcaggggcg actggctggt gaacctcgtc gtctgcggcc tgatcgtctg
     1381 cgggggcctg gggttcgtcg tgctcaggga acttgggcac gcgcggggcc ttcgccggct
     1441 ctcggtgcac acgcggctgg tgatcggcct gacggcgctc ctcatcgtcg gcgccacggc
     1501 cgcgctcttc ttcctggagg ggcggaaccc ggcgacgctc gcgcagctcc cgctgcacga
     1561 gcggctgctc gcgtcgttct tccaggccgt gacgccgcgc acggcgggct tcaacacgat
     1621 cgacatcggc gcgatggccc ccgccagcct cttcctgctc atgatcctga tgttcatcgg
     1681 ggcatcgccg ggcggcacgg gggggggcgt gaaggtgacg acgttcggga tcacggtggc
     1741 ggcgctggtg gcgaccgtgc gggggcgggc cgacaccacg ctcttccggc gaagggttgc
     1801 gcccgagatc gtgacgcgcg ccttcttcat cagcctgatc gccttcctcg cgctcaacac
     1861 gatcgcgggc ctgctcctgg tgttcgaggg ccggcagctc ctgccgaccc tcttcgagac
     1921 cacgagcgcg ttcggcacgg tgggcctgtc gatgggagag ggcgggccgc tcagcctcgc
     1981 gggccacttc tccgcccccg ggaagctgct gctgatggcg atgatgctgg ccgggcgcgt
     2041 cggcccgctc acgctggccg tggcgctggc cggcgggggg acgcccccgc gcgtccgcta
     2101 ccccgagggc agggtcctga tcggctgacg gagggtgacg tggtgcgacg aaagaagcag
     2161 ttcgcggtga tcgggctggg gcgcctcgga tcggccatgg cggccacgct cgccgaccta
     2221 ggccacgacg tcatcggggt ggacgcgaac gaggagcacg tgcgcgagct ggccgacgtg
     2281 ctcacgcagg cggtccagct cgacgccacc gacgagcgcg cgctccgcgc cgccggcatc
     2341 caggacgtgg acgtggcggt ggtttcgatc ggcgagaaca tcgagtcgag cctgctggtg
     2401 gtgatgcagc tcaaggagct gggcatcccg acgatcgtcg ccaaggccgt cacctcgctg
     2461 cacggccgga tcctcgagcg actgggcgtg tcgcgggtca tcttccccga gcgcgacatg
     2521 gcgatccggg tggcgcacag cctggtattg accgacgtga tcgactacat cgagctgtcg
     2581 cgcgacttca gcatcgtcga gatcccggct cccccgccgt tcgtgggccg cacgttgaag
     2641 gagctggagc ttcggcctcg cctcggcctc acgctcatcg caatcaagcg gcggccggcg
     2701 cccggcggcc ccgaggtcac gaccgtgtca cccggagcgg ccgagcggat ccagcagcac
     2761 gacgtgctcg tcctgctcgg ggacaacgcg cggatggcga agctgaacga gttgctcggg
     2821 tagcgagggc ccgccggtca gcgggcgtag aagtgcagca cctcgggcag gacgcggtgg
     2881 ctcttctcgg ggttggtgac ggcaccgcac gagtagcggt aaccggcgtg gatctcctgc
     2941 aacaggccga gcacctcgtg cgcgtacgcg agccgcgtct tcgagttctc gagcgcgacc
     3001 gcgaggcgcg ggtggtgccg cgcgatggtg ccccgggcgc cgcggatcgc gttcgcctcg
     3061 gcgttctcga tgtcgagctt gatgaagtcc acgcgctcga ccttcagctc cgccagcgcc
     3121 cggtcgagcg tggtcagcgg cacgtccacc gtcgccttca tccagggatg cgaaacgctg
     3181 ttgcacaccg agcacttctc gacgacgacc agcggaagcg tggcctcctc gtgccacacg
     3241 cccttctcga ccacgaccac cctgccctcg gcaatctcct tggcaaggtt gcgccgcagg
     3301 gcggcgttcg aacggggcgt cacctcgaag gtgatcacct tgccggcgcc ggcggccagg
     3361 gcggtacggg caaagaggcc gacgtgggct ccggcgtcca ccacgacgtc accgctgcgc
     3421 acgccgaggc gacccggccg gccgtacatg tcgtgctctt gctcggccag caccaccgag
     3481 aggctccaga ggtcgtcggc tgccggcatc cagaacttgc cggccggggt ctcgtacaag
     3541 tagagccccg acgacgggtc ctcctcgagg aaccggctgg catggaggat ctcgtcggtt
     3601 cgctgcttga cctcccgcgc cttcacgagg ccgcggaacg cgtcgagctc gccgcagagc
     3661 gggttctccc acgacacggc tgccttcgcg tccacccagt aggggccgta agcctggctg
     3721 cgggccgcga agacgagggc aaccgcgatc ccgaccgcga cgagcagcgc tccgatcaac
     3781 caccggcgct tgtgcatcgg ggttttatag cacacg
//
LOCUS       DAOPQX010000361         3811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26052_length_3811_cov_4.597977, whole genome shotgun sequence.
ACCESSION   DAOPQX010000361 DAOPQX010000000
VERSION     DAOPQX010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26052_length_3811_cov_4.597977"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1072)
                     /locus_tag="PKK95_15020"
     CDS             complement(137..1072)
                     /locus_tag="PKK95_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOC19578.1"
                     /translation="MQIIVTGSIAYDYLMSFPGSFSEHLLEGHLDRVSLSFLVDSMDK
                     RRGGCAPNISYTLALLGERPRLMGTAGQDFDEYRTWLEAAGVDTSLVRQVTGKFTASF
                     FCSTDKDGNQIASFYTGAMAHAGELSFRAAGPCDLAIVSPNDPGAMVQYAAECRTLGI
                     PYIFDPSQQVARLNGEELRAGVAGAEILICNDYEFAIIREKTGMDEADMLAHARALVV
                     TRGQRGSSIVLPSGAIDIPAVPPRQIVDPTGVGDAFRGGFMKGLAAGAGLEVCGRLGS
                     VAAAYALEHLGGSSHAYTLEEFLERYAETFGKEPY"
     gene            complement(1090..1950)
                     /gene="mtnP"
                     /locus_tag="PKK95_15025"
     CDS             complement(1090..1950)
                     /gene="mtnP"
                     /locus_tag="PKK95_15025"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOC19579.1"
                     /translation="MTAQIGIIGGSGLYDMAELTGREEVAIETPFGSPSGPFVLATLR
                     GRRVAFLARHGAGHRISPSELNFRANVYGFKVLGVERIISASAVGSLKEAYPPLDIVI
                     PDQFFDRTKGRPSTFFGRGIVAHVGFAHPVCRELAGVVHESARACGARAHLGGTYVCI
                     EGPQFSTEAESRLYRSWGMDVIGMTNLQEAKLAREAEICYSTLALVTDFDCWHPEHDT
                     VSVEMIVANLMQNARTAQQVIAEAVGRIPASRGCACGSALATAIITSPEAIPAQVKDE
                     LRPIIGKYVK"
     gene            complement(1993..>3811)
                     /locus_tag="PKK95_15030"
     CDS             complement(1993..>3811)
                     /locus_tag="PKK95_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19580.1"
                     /translation="LAAVLPWASHLGGVVSFDVGETKGHEGDTVLFSKWNSFSRVAVY
                     DRRHGDWSLSPAYTGPMPDSRFMDIDSAASTPIVRFTGRVEDGAYLRSELTSIAYHLV
                     NPQAGGLDGNGGENRPFSALVIGPGGGRDLLAALAFGASRVDGVEINAIIARDVMLDR
                     FRDYSGDIYRWPGVRVHVEDGRSFVRRSGDRYEVIQASLVDTWAATAAGAYTLTENSL
                     YTVEAFEDYLDHLSERGLLTITRWAVDGLRLVSLAQEACARRGWTAHDRLAIVQHEKV
                     ATFILKRSPFTSGEVRHLEEVARALRFTILYAPGLPAPEVLAPTGTGHWEAAAGTPND
                     YARLIAAPDRHAFYQSYATDITPTTDDRPFFFHTTKLKDQLGVAFGRSMLFGNGLSAL
                     ITLIGISGALVLLFVLAPLALGGRSVLGTGWPRWLGYFGLLGAAFMLVEVALLQRFVL
                     LLGHPVYSLAVTLFSLLLGTGIGSFLSRRLPDARLGRSLVLALAGLAVLGLVAIVALP
                     PLIRAAIVLPLGVRLALAAAVLLPAGMLMGVPLPAGVRLLSSRTPELLPWAWAMNGAL
                     SVLGATLAVFIAMNWGFSTTLVAGGALYLLAAAVVPRPL"
BASE COUNT          601 a         1359 c         1324 g          527 t
ORIGIN      
        1 cgcacctccg gcaccttcag cactgcaccc gccttcgccg cgcggtgcgc ggctacggcg
       61 cggcagacct ccggcacctc cagcaccgca cctccggcac ctcctgcacc ttctgcacct
      121 tcagcacgat ccgatttcag tacggctctt tgccaaacgt ttccgcgtac cgctcgagga
      181 actcctcgag cgtgtaggcg tggctcgacc cgccgaggtg ttcgagcgcg taggcggccg
      241 cgacgcttcc gagccggccg cagacctcga ggccggcccc cgcggccagc cccttcatga
      301 agccgccgcg aaacgcgtcg ccgacgccgg tcgggtcgac gatctgccgc ggaggcacgg
      361 cgggaatgtc gatcgcgccg gaggggagga cgatcgagga gccgcgctgg ccgcgggtca
      421 ccacgagcgc gcgcgcgtgc gccagcatgt cggcctcgtc catcccggtc ttctcgcgga
      481 tgatggcgaa ctcgtagtcg ttgcagatca ggatctcggc ccccgccacc cccgcgcgca
      541 gttcctcgcc gttgaggcgg gccacctgct ggctcgggtc gaagatgtag ggaatcccca
      601 gcgtgcggca ctcggccgcg tactgcacca tcgcccccgg gtcgttcggc gagacgatcg
      661 ccaggtcgca ggggccggcc gcccggaacg acagctcgcc cgcatgcgcc atcgcccccg
      721 tgtagaacga cgcgatctgg ttgccgtcct tgtcggtgct gcagaagaag gaggcggtga
      781 acttgccggt cacctgccgg acgagcgagg tgtccactcc cgccgcctcc agccaggtgc
      841 ggtactcgtc gaagtcctgt cccgcggttc ccatcagccg ggggcgctcg ccgagcagcg
      901 ccagcgtgta cgagatgttc ggcgcgcacc cgccgcggcg cttgtccatc gagtccacga
      961 ggaagctgag gctgacccgg tcgaggtgtc cctcgagcag gtgctcgctg aagctcccgg
     1021 gaaacgacat caggtagtcg taggcgatcg agccggtgac gatgatttgc atggtgactt
     1081 cacgtgcttc tacttcacgt acttcccgat gatcggcctc agttcgtcct tcacctgcgc
     1141 cgggatcgcc tcggggctcg tgatgatcgc cgtcgccagc gcgctgccgc aggcgcaccc
     1201 gcgggatgcc gggatccgcc ccacggcctc tgcgatcacc tgctgcgccg tgcgcgcgtt
     1261 ctgcatcagg ttcgcgacga tcatctcgac gctcaccgtg tcgtgctccg ggtgccagca
     1321 gtcgaagtcg gtgaccaggg cgagcgtcga gtagcagatc tcggcctcgc gcgcgagctt
     1381 cgcctcctgc aggttggtca tcccgatgac gtccatgccc cagctgcggt agaggcgcga
     1441 ctcggcctcg gtggagaact gcggcccctc gatgcagacg taggtccccc cgaggtgtgc
     1501 cctcgcgccg caggcgcgcg ccgactcgtg caccacgccg gccagctcgc ggcagacagg
     1561 gtgagcgaag ccgacgtgcg caacgatgcc gcggccgaag aaggtgctcg gcctcccctt
     1621 cgtgcggtcg aagaactgat cggggatcac gatgtcgagc gggggatagg cctccttcag
     1681 gctccccacc gcgctcgccg agatgatccg ttcgacgccg agcaccttga agccgtagac
     1741 gttcgcccgg aagttcaact cggacggaga aatccggtgc cccgcgccgt gccgcgcgag
     1801 gaaggccacg cggcggccgc ggagggttgc gaggacgaag gggcccgagg gagagccgaa
     1861 cggcgtctcg atcgcgacct cctcgcgacc tgtcagctcc gccatgtcgt acaggccgct
     1921 tcccccgatg atgccaatct gcgccgtcat gtcgctcctc atcgcgtcgg tgggggggca
     1981 acaggcaggt gattatagcg gacgcggcac gacggccgcg gcgagcaggt acagcgcgcc
     2041 gcccgcgacg agcgtggtcg agaaccccca gttcatcgcg atgaagaccg cgagggtggc
     2101 gcccagcacc gagagcgccc cgttcatggc ccacgcccag ggcagcagct ccggcgtccg
     2161 cgacgacagc agccggacac cggccggcag cggcaccccc atcagcatcc cggccggcag
     2221 cagaacggcc gccgccagcg cgaggcgcac gccgagcggc agcacgatgg ccgcccggat
     2281 cagcggcggc agcgcgacga tcgccacgag gcccagcacc gcgaggccgg caagcgccag
     2341 gaccaggctc cggcccaggc gggcgtcggg cagccggcgg ctcaggaagc tcccgatccc
     2401 ggtgccgagc agcagcgaga acaaggtgac cgccagcgag tacaccgggt gcccgagcag
     2461 cagcacgaac cgctgcagca gggcgacctc gaccagcatg aacgccgccc cgagcaggcc
     2521 gaagtagccc agccagcgcg gccagcccgt tccgagcacg cttcgtccgc cgagggcgag
     2581 cggcgccagc acgaacagca gcacgagggc gccgctgatc ccgatgagcg tgatcagggc
     2641 gctgagcccg ttgccgaaca gcatcgaccg gccgaacgcc acgccgagct ggtccttcag
     2701 cttggtcgtg tggaagaaga acgggcggtc gtcggtagtg ggcgtgatgt cggtcgcgta
     2761 cgactggtag aaggcgtgcc ggtcgggggc cgcgatgagc cgggcgtagt cgttgggggt
     2821 gccggcggcc gcctcccagt gcccagtccc ggtcggcgcg agcacttccg gcgccggaag
     2881 gccgggcgcg tagaggatcg tgaagcgcag cgcccgggcc acctcctcga ggtgccgcac
     2941 ctcgccggag gtgaagggcg agcgcttgag gatgaaggtg gccaccttct cgtgctgcac
     3001 gatcgcgagg cgatcgtgcg cggtccagcc gcgccgcgcg caggcctcct gagccagcga
     3061 cacgagccga aggccgtcca ccgcccagcg cgtgatggtc agcaggcccc gctcggacag
     3121 gtgatcgagg tagtcctcga acgcctcgac cgtgtagagg ctgttctccg tgagcgtgta
     3181 ggctcccgct gccgtcgccg cccatgtgtc caccagcgag gcctggatca cctcgtagcg
     3241 atcgcccgag cggcggacga agctgcggcc gtcctccacg tggacccgca cgccgggcca
     3301 gcggtagatg tcgcccgagt agtcgcggaa ccgatccagc atcacgtcgc gggcgatgat
     3361 cgcgttgatc tccacgccgt ccacgcggct ggcaccgaag gcgagcgccg cgagcaggtc
     3421 gcgcccgccg ccggggccga tcaccagcgc cgagaagggc cggttctccc cgccgtttcc
     3481 gtccaggccg cccgcctggg ggttgacgag gtggtacgcg atcgaggtca gctccgaccg
     3541 caggtacgcc ccgtcctcca cccgcccggt gaaccggacg attggggtcg aggccgccga
     3601 gtcgatgtcc atgaagcggg agtcgggcat cggcccggtg taggcggggc tcagcgacca
     3661 gtccccgtgg cggcggtcgt acacggcgac gcgtgagaac gagttccact tgctgaacaa
     3721 gacggtgtcg ccctcgtgcc ccttggtctc gcccacgtcg aacgacacca cgccgccgag
     3781 gtgcgaggcc cagggaagca cggccgcgag c
//
LOCUS       DAOPQX010000362         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26316_length_3783_cov_4.274678, whole genome shotgun sequence.
ACCESSION   DAOPQX010000362 DAOPQX010000000
VERSION     DAOPQX010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26316_length_3783_cov_4.274678"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PKK95_15035"
     CDS             complement(<1..1288)
                     /locus_tag="PKK95_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOC19581.1"
                     /translation="MSNSTSATTESRSRRRAARVVLRVCAAAAFALVILGVVVWRALT
                     ASLPVVDGTRRLPGLSAPVTVERDGAGVPSISGLTRVDVARATGFVHAQDRYFQMDLT
                     RRFASGELAELVGEAGLPVDRALRRHRFRAVAAEAVRRASPGEQRLLEAYAAGVNEGL
                     DALGAVPVEYLLLRSDPAPWRTEDSFLVLAFMYVGLQRGAMWREPMMGLMRERLPAAL
                     AAFLEPGPDQWEAPIAGTPPDRPPLPTEEQASVRAGWPDVPVPGRGAARLQHPEAMHG
                     SNSWAIAPEHTARGAALLANDMHLSIQVPNAWYRVVLAWPDTSAPGGRRRLAGVSLPG
                     VPAVVVGSNGRVAWGFTNAGIDATDYVLLEPADGEPGSYRTPAGAATLVVHEERIRCK
                     GRPDEVLKVEETMWGPVFDSDEQGRRRALAWVAQRPE"
     gene            complement(1254..1748)
                     /locus_tag="PKK95_15040"
     CDS             complement(1254..1748)
                     /locus_tag="PKK95_15040"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOC19582.1"
                     /translation="MPSFDVTSEVDLQEVDNAVNQARKEIVQRFDFKGSKAAIEFDRA
                     GATLTLTADDEFRMQALWEVVETRLVRRKVPLKNVKRGDLERGANDTVRRVVTLQQGV
                     PTEASREIVKFIKDRKLRKVQAAIQGDQLRISSPSRDELQQVIALLRQQDFGVELHFG
                     NYRE"
     gene            complement(1766..2965)
                     /locus_tag="PKK95_15045"
     CDS             complement(1766..2965)
                     /locus_tag="PKK95_15045"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOC19583.1"
                     /translation="MTMRTYLVFATVLPLVLAGAFAWAQQPGPSRRPAPPYSPARVAG
                     DFVYLSGMLPTGDDGTIVPGDIKAQTARTLDNLAAVLRRNGSSLERAASVSVYLRRAS
                     DFAAMNEVYARYWPKDPPARTTVMAPPVLPDALVEISLVAVRDGAERTIVHPEGWLRS
                     PSPYSYGVKSGNTLFLAGLVARRANDNTFAGGDMKAQTAAAIQNGREILSAAGFSLAD
                     VVSSRVYITNTAFVQDMNGVYREAFPVSPPARATVRCGLMGQDYLVEITMVAVRDPER
                     RAITTPGPDGSPGKASPVLSSAIQVGNRLYLSGMLGVTDAARGNVAAQTNETLGRMGR
                     TLEAAGFSWKNVVEAVVYLPDARDFAAMNEAYRAALPEPFPARTTVEAGLVAPDGLVE
                     IMMTAVK"
     gene            3040..3432
                     /locus_tag="PKK95_15050"
     CDS             3040..3432
                     /locus_tag="PKK95_15050"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOC19584.1"
                     /translation="MIERRRSRLHGWGVFATERIPKNKRIIHYAGEKITHRESREREE
                     RYLKRGKIWCFRLNNRWVIDGAVGGNLARFVNHSCKPNCYAQVIDGVIWLRASKTIEP
                     GEELTYDYKTDGAKSIPCRCRPGCATRL"
     gene            3434..>3783
                     /locus_tag="PKK95_15055"
     CDS             3434..>3783
                     /locus_tag="PKK95_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOC19585.1"
                     /translation="MLAALVAAVVTGVSAAAPPRAAVLLIDLRDGPKGATLLEERPDI
                     LDAPVAPGSIAKILTLVAALESATITPGSRITCPGRATIAGETYTCTHPRTGRPLSPA
                     EALAHSCNTYFATIA"
BASE COUNT          581 a         1321 c         1351 g          530 t
ORIGIN      
        1 cctcggggcg ctgcgccacc caggccaggg cacgccgccg gccctgctcg tcgctgtcga
       61 agacgggccc ccacatcgtc tcctcgacct tcaagacttc gtcgggccgt cccttgcacc
      121 ggatgcgctc ctcgtgcacc acgagggtcg cggcgccggc gggcgtgcga tacgagcctg
      181 gctcgccgtc agctggctcg agcagcacgt agtcggtggc gtcgatcccg gcattcgtga
      241 agccccaggc gacgcggccg ttcgacccga cgacaaccgc ggggacaccg ggaaggctga
      301 ccccggcaag gcggcgccgg ccgccaggag ccgacgtgtc gggccacgcc agcacgaccc
      361 ggtaccaggc gttgggcacc tggatgctca ggtgcatgtc gttggcaagg agcgccgctc
      421 cccgggcggt gtgctcgggc gcgatcgccc acgagttgct gccgtgcatt gcctcggggt
      481 gctggaggcg ggccgcgccg cgcccgggga ccggcacatc gggccagccg gcacggacgc
      541 tggcctgctc ctcggtgggc aggggaggac gatcgggagg cgtcccggca atcggcgcct
      601 cccactggtc gggaccgggc tcgaggaacg cggcaagcgc ggccggcagg cgctcgcgca
      661 tcaggcccat catcggctcg cgccacatgg cgccgcgctg aaggccgacg tacatgaagg
      721 cgagcacgag gaagctgtcc tcggtccgcc agggcgccgg atccgagcgc agcagcaggt
      781 actccacggg gacggcgccg agcgcgtcga gcccctcgtt gacgccggct gcgtacgcct
      841 ccagcaggcg ctgttcgccg gggctcgcac gccgaacggc ctcggcggcc acggcgcgga
      901 agcggtgccg ccgcagtgca cgatccacgg gcaagcccgc ttcgcctacc agctcggcca
      961 gctctcccga cgcaaagcgg cgggtgagat ccatctggaa gtagcggtcc tgggcgtgga
     1021 cgaacccggt cgcccgggcc acgtccacac gcgtaaggcc gctgatcgag gggacgccgg
     1081 cgccgtcgcg ctcgacggtg acgggtgccg agagccccgg caggcgccgc gtgccgtcga
     1141 cgaccggcag gcttgcggtc agcgcgcgcc agacgacgac gccgaggatc accagggcga
     1201 aggcggccgc cgcgcaaacc cgcaggacga cgcgcgcggc ccgccggcgc gacctactct
     1261 cggtagttgc cgaagtggag ttcgacaccg aagtcctgct ggcggagaag cgcgatcacc
     1321 tgctgcagct cgtcccgcga cggggatgag atgcgcagct ggtcgccctg gatggcggcc
     1381 tggaccttcc tgagcttccg gtccttgatg aacttcacga tctctcgaga cgcctcggta
     1441 ggcaccccct gctggagggt cacgacacgg cggacggtgt cgttcgcgcc gcgctcgagg
     1501 tcgccgcgct tgacgttctt gagcggcacc ttccgccgga cgagcctcgt ctccaccact
     1561 tcccacagcg cctgcatcct gaactcgtcg tccgcggtga gggtgagcgt ggccccggcg
     1621 cgatcgaact cgatcgcggc cttcgagccc ttgaagtcga agcgctgcac gatctccttg
     1681 cgcgcctggt tgacggcgtt gtccacttcc tggaggtcga cctcggacgt cacgtcgaat
     1741 gacggcatag ggaggatcct agcccctact tcaccgcggt catcatgatt tcgacgagcc
     1801 cgtcgggcgc caccaggccc gcctccaccg tggtgcgcgc ggggaacggc tcgggcagcg
     1861 cggcgcgata cgcctcgttc atcgcggcga agtcgcgtgc atcggggagg tagacgaccg
     1921 cctcgaccac gttcttccag gagaagccag ccgcctcgag tgtccgcccc atccgcccga
     1981 gcgtttcgtt ggtctgcgcg gcaacgttgc ctcgggctgc atccgtcacc ccgagcatgc
     2041 ccgagaggta gaggcggtta ccaacctgga tcgcggagct gaggaccggg ctggccttgc
     2101 caggcgagcc gtcgggcccg ggggtcgtga tggcgcggcg ctccgggtcg cgaacggcga
     2161 ccatggtgat ctcgacgagg tagtcctggc ccatcaggcc gcaccggacg gtcgcgcgcg
     2221 ccggcggcga gaccggaaac gcctcgcgat acacgccgtt catgtcctgg acgaacgcgg
     2281 tgttggtgat gtacacgcgc gaggaaacga cgtccgcaag cgagaagccg gccgccgaca
     2341 ggatctcgcg gccgttctgg atggcggcgg ccgtctgggc cttcatgtcg ccgccggcga
     2401 acgtgttgtc gttggcgcgc ctggcgacga gacccgccag gaagagggtg ttgcccgact
     2461 tgacgccgta gctgtacggg ctgggggatc gcagccagcc ctcgggatgg acgatcgtcc
     2521 gctccgcgcc gtcgcgcacg gcgacgaggc tgatctccac gagggcgtcc ggcagcacgg
     2581 gcggcgccat caccgtggtc cgcgcggggg gatccttggg ccagtaacgc gcgtagacct
     2641 cgttcatggc ggcgaagtcg gaagcgcggc gcaagtacac ggagaccgag gcggcccgct
     2701 cgaggctcga cccgttgcgg cggagcacag ccgcgagatt gtcgagcgtg cgcgctgtct
     2761 gggccttgat gtcgccagga acgattgtcc cgtcgtctcc cgtcgggagc atgcccgaga
     2821 ggtagacgaa gtcgccggcc acgcgcgcgg gcgaataggg cggtgcgggc cgcctggaag
     2881 gcccgggctg ctgggcccag gcgaaggcgc cggcgagcac gagcggaagc accgtggcga
     2941 acaccaggta cgtgcgcatg gtcatcccct gtcgccggcg gccgccttcg cgaaaatcgg
     3001 cgagccaggc gggccggcgc tactggtaca atcgtgtcga tgattgaacg gcgtcgctcg
     3061 agactccatg ggtggggcgt gttcgcgaca gaacgcatcc cgaagaacaa gcgcattatc
     3121 cactacgccg gggagaagat cacccaccgg gagagccgcg agcgcgagga gcgctacttg
     3181 aagcgcggca agatctggtg cttccggctg aacaaccggt gggtgatcga cggcgcggtc
     3241 ggcggcaacc tggcgcgctt cgtcaaccac tcctgcaagc cgaactgcta cgcccaggtg
     3301 atcgacgggg tgatctggct ccgcgcctcg aaaacgatcg agccgggcga ggagctcacc
     3361 tacgactaca agacggacgg cgccaagagc attccgtgcc gctgccggcc cggctgcgcc
     3421 acgcggctgt aggttgctcg ctgcgctggt cgccgcggtg gtgaccgggg tctcggcggc
     3481 ggcaccgccg cgcgcggccg tcctcctgat cgacctgcgc gacgggccga agggcgccac
     3541 gcttctcgag gagcggcccg acatcctgga cgcgcccgtt gcccccggct cgatcgcgaa
     3601 gatcctcaca ctggtggcgg ccctcgagtc ggcgaccatc acccccggga gcaggatcac
     3661 ctgccccgga cgggcgacca tcgccggcga gacctacacg tgcacgcatc cgcgaacagg
     3721 ccgccccctg tcgccggccg aggcgcttgc ccactcctgc aacacctact tcgccacgat
     3781 cgc
//
LOCUS       DAOPQX010000363         3769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26452_length_3769_cov_4.563005, whole genome shotgun sequence.
ACCESSION   DAOPQX010000363 DAOPQX010000000
VERSION     DAOPQX010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26452_length_3769_cov_4.563005"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKK95_15060"
     CDS             <1..745
                     /locus_tag="PKK95_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOC19586.1"
                     /translation="GFTGIWGAAAIIFFAYIGFDAVSTAAEETRNPQRDMPVGMIASL
                     VICTVIYIAVAIVLTGLLHWEQLGTAQPLATAFSAIGMNWAAGVISLGAVFACTSVLM
                     VFQLGQPRIFFSMARDGLLPPWAAKIHPKYRTPHVTTILTGIVVALFASVTNINEVVE
                     LTNIGTLFAFALVSAGIIVLRHVDPGRKRPFRTPLVPLVPLLSIFSCLWLAVQLPAVT
                     WWRFIAWLAGGLVLYFLYGHRHSRLREGN"
     gene            complement(750..1997)
                     /locus_tag="PKK95_15065"
     CDS             complement(750..1997)
                     /locus_tag="PKK95_15065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOC19587.1"
                     /translation="MTPTDTAGFRAASTTYRAPILSPAAAAWDHWPDGRLVVRGGKVA
                     EAGPWRRGTRADVDLRPHFVFPGLVDVHAHVPQLPVCGVHPGSLLEWLSRWTFPLERQ
                     FKGGRARELSREYFREMLAQGTTTAALYTSAWPDSVEACFEEAAALNLHAWIGPPLMD
                     VDAYRPVTTRRVLDEARALARLDSPERRFAVTPRFALSATRELLAGCAALARERGLPI
                     QTHLAEQRAECDAVRERFGQGYLDVYDEAGLLTPRTIFAHSIWLSAHEWRRLARAGCS
                     VAHCPTSNVFLGSGVMNWKRARQTRVGLGSDVGAGPDLSMFRVARMAWTLHSLNGAPA
                     DAGELLRAATSGGAQALGFADVGALAPGCSADFLVLDAAQIVPPGAPRAESTEDLLSR
                     ILHRAGRSAVRQVYVAGILRHER"
     gene            2074..>3769
                     /gene="uvrA"
                     /locus_tag="PKK95_15070"
     CDS             2074..>3769
                     /gene="uvrA"
                     /locus_tag="PKK95_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOC19588.1"
                     /translation="MGHDWIAVRGARVHNLKSIDVDLPRNRLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLEQMEKPDVDLIDGLSPAISIEQKTTASNPRSTVGT
                     VTEVYDYLRLLFANVGVPHCHQCGREIASQSLEQIVERVLEFPRDERINVLAPIVRGR
                     KGEFKKELAALRARGFSRARVDGEMRSLDEEIALDRRRNHSIDVVVDRLLVKPGIERR
                     LSDSVDTALGLSDDVVIINGLEAGDRLFSRRLACVECGISMPEMTPRAFSFNSPHGAC
                     PECQGLGALYDFDPARVVPDPSRSLAGGAIAPWASGDARLVREALDSLSRYYGIDLSV
                     PFGKLPKKARDLLLEGPDAKRGRPAEGGRPHRRAAASDPFGRDFEGILPNLRRRFEEG
                     SWLDQEALERFRSLRSCPSCGGARLKPQSRSVRLKGRTITEYVDLPLDDALKVFQAMT
                     LTPREELIAGRVLREVQDRLRFLTDVGVGYLTLGRSAATLSGGEGQRIRLATQIGAAL
                     RGVLYVLDEPSIGLHQRDNRRLLATLRRLRDLGNTVIVVEHDEETIRTADYLVDLGP"
BASE COUNT          528 a         1392 c         1306 g          543 t
ORIGIN      
        1 cggcttcacc gggatctggg gggccgccgc gatcatcttc ttcgcctaca tcggcttcga
       61 cgccgtctcg accgccgccg aggagacgcg caacccccag cgcgacatgc ccgtcgggat
      121 gatcgcgagc ctggtgatct gcaccgtcat ctacatcgcc gtcgccatcg tgctcaccgg
      181 tctgctgcac tgggagcagc tgggcaccgc gcagccgctg gccacggcgt tctcggcgat
      241 cgggatgaac tgggccgccg gcgtgatctc gctcggcgcc gtcttcgcct gcacctcggt
      301 gctcatggtg ttccagctcg gccagccgcg tatcttcttc tcgatggcgc gcgacggcct
      361 gctgccgccg tgggcggcga agatccaccc gaagtaccgc acgccgcacg tcaccacgat
      421 cctcaccggc atcgtcgtcg cgctgttcgc gagcgtcacc aacatcaacg aggtggtgga
      481 gctgacgaac atcggcacgc tcttcgcctt cgcgctcgtc tcggccggca tcatcgtcct
      541 gcggcacgtg gaccccggcc ggaagcgccc cttccgcacg ccgcttgtcc cgctcgtgcc
      601 gctgctgtcg atcttcagct gcctgtggct cgcggtgcag ttgccggccg tcacctggtg
      661 gcgcttcatc gcctggctgg cgggagggct ggtgctgtac ttcctgtacg gccaccgcca
      721 cagccggctg cgggagggga actgacgcct caacgctcgt ggcgcaggat gccagccacg
      781 tagacctggc gcacggccga gcggccggcg cggtgcagga tccgcgacag gaggtcctcg
      841 gtcgactcgg cccgcggcgc ccccggcggt acgatctgcg cggcgtcgag gacgaggaag
      901 tcggcgctgc accccggagc gagcgcgccg acgtcggcga acccgagggc ctgcgccccg
      961 ccagaagtcg ccgccctgag cagctcaccc gcgtcggccg gggcgccgtt cagcgagtgc
     1021 agcgtccacg ccatccgcgc cacgcggaac atcgagaggt cgggcccggc tcccacgtcc
     1081 gatcccagcc cgacgcgcgt ttgccgcgcg cgcttccagt tcatgacgcc gctgccgagg
     1141 aagacgttcg aggtggggca gtgggccacc gagcagccgg cccgggccag ccgccgccac
     1201 tcgtgcgccg acagccagat cgagtgcgcg aagatcgtgc gcggggtcaa aaggccggct
     1261 tcgtcgtaga cgtcgaggta gccctgcccg aagcgctcgc gcaccgcgtc gcactcggcc
     1321 cgctgctcgg cgaggtgagt ctggatcggc agcccgcgct cgcgggcgag cgccgcgcac
     1381 cctgccagca gctcgcgcgt cgcagaaagg gcgaaccgcg gggtgaccgc gaaacggcgt
     1441 tccggcgagt cgaggcgggc gagcgccctc gcctcgtcca gtactcggcg ggtggtcacc
     1501 ggccggtacg cgtccacgtc catcagcggc gggccaatcc acgcgtgcag gttgagggcg
     1561 gccgcctcct cgaagcacgc ctccaccgag tcgggccagg ccgaggtgta gagcgccgcg
     1621 gtcgtggtgc cctgcgccag catctcgcgg aagtactccc gcgacagctc ccgcgcccgg
     1681 ccgcccttga actggcgctc gagggggaag gtccagcggc tcagccactc cagcaggctg
     1741 ccggggtgca ccccgcagac cggcagctgc ggcacgtgcg catggacgtc aaccaggccc
     1801 gggaagacga agtgcggccg caggtcgacg tcggcccggg tcccgcggcg ccatggacca
     1861 gcttcggcca ccttgccgcc ccgaacgacc aggcggccgt cgggccaatg gtcccacgct
     1921 gcggcggccg gcgacaggat gggggcccta taggtggtgg atgcggcgcg aaacccggcg
     1981 gtatcggtcg gcgtcatgtc gaaccccggc cgattatacg cggcgtggta caatcggcgg
     2041 ttcggggccc ccgtacaggg ccgcccgtag agcatgggtc acgactggat tgccgtacgc
     2101 ggcgcccgcg tccacaacct gaagagcata gacgtggacc tgccccgcaa ccggctggtg
     2161 gtcatcaccg gcctgtcggg gtcggggaag tcgtcgctcg ccttcgacac gatctacgcc
     2221 gagggccagc ggcggtacgt cgagtcgctc tcggcctacg cgcggcagtt cctcgagcag
     2281 atggagaagc cggacgtcga cctgatcgac ggcctctcgc cggccatctc gatcgagcag
     2341 aagaccaccg cgtcgaaccc ccgctcgacc gtcggcaccg tcaccgaggt ctacgactac
     2401 ctccggctgc tcttcgccaa cgtcggcgtc ccgcactgcc accagtgcgg ccgcgagatc
     2461 gcgtcgcagt cgctcgagca gatcgtcgag cgcgtcctcg agttcccgcg cgacgagcgg
     2521 atcaacgtgc tggcgccgat cgttcgcggc cggaaggggg agttcaagaa ggagctggcg
     2581 gcgctgcggg cacgcggctt cagccgcgcc cgggtcgacg gcgagatgcg gtcgctcgac
     2641 gaggagatcg ccctcgatcg gcggcgcaac cactcgatcg acgtcgtggt cgatcgcctg
     2701 ctcgtgaagc ctggaatcga acgccgcctg tccgactcgg tggacaccgc gctcggcctc
     2761 tccgacgacg tcgtcatcat caacgggctg gaggcgggtg accggctgtt ctcgcggcgc
     2821 ctggcctgcg tcgaatgcgg catcagcatg cccgagatga cgccccgcgc cttctcgttc
     2881 aactcgccgc acggcgcctg tcccgagtgc cagggcctgg gggcgctcta cgacttcgac
     2941 ccggcccgcg tcgtccccga cccctcgcgc tcgctcgccg gaggagccat tgcgccctgg
     3001 gcttcgggcg acgcgcggct cgtgcgcgag gcgctcgact cgctttcccg ctactacggg
     3061 atcgaccttt ccgtcccctt cggcaagctg ccgaagaagg cgcgcgacct cctgctcgag
     3121 gggcccgacg ccaagcgcgg ccggccagcc gagggcgggc gcccccaccg ccgggcggcg
     3181 gcctccgacc cgttcgggcg cgacttcgag gggatcctgc cgaacctgcg gcggcgcttc
     3241 gaggagggga gctggctcga ccaggaggcg ctcgagcgct tccgctcgct gcgctcctgc
     3301 ccctcctgcg gcggcgccag gctgaagccg cagagccgca gcgttcgcct caagggacgc
     3361 acgatcaccg agtacgtgga cctccccctc gacgacgccc tgaaggtctt ccaggcgatg
     3421 acgctgacgc cgcgcgagga gctgatcgcc gggcgcgtgc tgcgcgaggt ccaggaccgc
     3481 ctgcgcttcc tcaccgacgt cggggtcggg tacctgacgc tcggccgcag cgccgccacg
     3541 ctctcgggcg gcgagggtca gcgcatccgg ctcgccaccc agatcggcgc cgcgctccgc
     3601 ggtgtgctct acgtgctcga cgagccgtcg atcggcctgc accagcgcga caaccggcgt
     3661 ctgctggcga cgctccgccg cctgcgcgac ctcggcaaca cggtcatcgt ggtcgagcac
     3721 gacgaggaga cgatccgcac ggccgactac ctggtggacc tcgggccgg
//
LOCUS       DAOPQX010000364         3748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26653_length_3748_cov_3.742757, whole genome shotgun sequence.
ACCESSION   DAOPQX010000364 DAOPQX010000000
VERSION     DAOPQX010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26653_length_3748_cov_3.742757"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKK95_15075"
     CDS             complement(<1..409)
                     /locus_tag="PKK95_15075"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOC19589.1"
                     /translation="MTLLERVGAGVVIGDGAMGTELQRAGLPIGECGEAWTLANPGVL
                     QGIHEAYLAAGSELILTNTFGANPWVLERYGLAAEYERFNREIAGIARRAAARRAVVL
                     GDIGPCGHFLRPLGDVDAAELSEAFGRQARALLE"
     gene            complement(406..2271)
                     /locus_tag="PKK95_15080"
     CDS             complement(406..2271)
                     /locus_tag="PKK95_15080"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOC19590.1"
                     /translation="MDREPFDVRFLPDESVCRATGPLGLDVAAARAGILLEHPCGAQG
                     TCGRCRVRVRSGPAPITEDDRDQIGPRDLEEGWRLGCRLVLDGPATIEVPPVSRSLAG
                     KSFGDPVEPELLARPVVERLAVVLPPNDLSEGALLDRLSSACGLASPLAAKPAVLAEL
                     GRAWAAGQPLTMTLEGNELVAASRDEVRSGFGLAVDIGTTSLAAALVDLSDGSVRASG
                     ARLNPQVAFGSDVIARIRHAMERDEHLAALAGAVRGGLAGLVKDLVAEVGCDLRDVVV
                     AAVAGNPTMLHAWAGVPIVGLGMAPYAAVWAGQACWKAAEVELAIHPNANVFVFPMIR
                     SHVGGDAVAAAIACDIDQPGEPRLLIDLGTNTELLAVSGERAVVTSAAAGPAFEGVSI
                     RCGMRAAPGAIDLVSLPGDGQVLVNVIGGVAANGLCGSGIVDAVAELLRVGILTPSGA
                     MRKASELPASAAGYGSRLRQIDGQQAFVLASPDEGAAREVVCTARDVREIQLAKGAIL
                     TAARLACRKLGLALDEMAEVLVAGAFGNVLRKVSVRGIGLLPGVEPERIRLVGNAAGI
                     GARLALLDAEVRQRASRFARHAEYIDLATDAAYQDTFLRALALPDLGASGAGGGR"
     gene            2320..2523
                     /locus_tag="PKK95_15085"
     CDS             2320..2523
                     /locus_tag="PKK95_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19591.1"
                     /translation="MAGDRIQQAIVSVPGVQEVLKVLEVLVPEVLKACRAGAAHRSAK
                     AGAGGAGAGGAEGCRAVAAACPP"
     gene            2619..2987
                     /gene="hypA"
                     /locus_tag="PKK95_15090"
     CDS             2619..2987
                     /gene="hypA"
                     /locus_tag="PKK95_15090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOC19592.1"
                     /translation="MHELSIAESIADSVRRHPALQGGRKVRRIGVQVGDTSGVNAEAL
                     DFCFTIVVRGTELDGAALDLERVPVRFRCETCGHEFEPVEFDPRCPACGAERGRLSGG
                     DELSLAYLELDDEEPGETRE"
     gene            2984..3676
                     /gene="hypB"
                     /locus_tag="PKK95_15095"
     CDS             2984..3676
                     /gene="hypB"
                     /locus_tag="PKK95_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOC19593.1"
                     /translation="MTERVTVRENVLKENDRIAAGLRAAFDRGAIFCVNLISSPGAGK
                     TSILEGTLEALQGTAVRAAVLTGDIQTERDADRLRRHGFPALQITTGGTCHLDAAMVR
                     RALDSLSLDAMDVLFIENVGNLVCPASYDLGEDVKVVVLSVTEGDDKPLKYPGIFRRA
                     GLMLLHKMDLLPHARFDPAAAVADARRVQPDLEVVETSCYGAPGLEAWVGWVRSRLDA
                     KRAAGRTLSAEP"
BASE COUNT          577 a         1346 c         1340 g          485 t
ORIGIN      
        1 cctcgagcag cgcccgggcc tgccgcccga atgcctcgga cagctcggcc gcatccacgt
       61 cgcccagcgg ccggaggaag tggccgcacg gaccgatgtc accgagaacc accgcgcggc
      121 gcgcggcggc ccgccgcgcg ataccggcga tctcgcgatt gaagcgctcg tactcggcgg
      181 caaggccgta gcgctcgagc acccacgggt tcgcgccgaa cgtgttggtg aggatcagct
      241 cgctgccggc ggcgaggtag gcctcgtgga tcccctgcag gacgcccggg ttcgccagcg
      301 tccacgcctc accgcactcc ccgatcggca ggccggcccg ctgcagctcc gtgcccatcg
      361 cgccgtcccc tatcaccacc ccggctccaa ccctctcgag cagcgtcatc gtccacctcc
      421 agcaccggaa gccccgaggt cggggagggc cagcgcccgc aagaacgtgt cctggtaggc
      481 cgcgtcggtt gccaggtcga tgtattccgc gtgccgcgcg aagcgcgagg cgcgctgcct
      541 gacctcggcg tcgagcaggg ccagccgggc gccaatcccg gccgcgttgc cgacaagcct
      601 gatccgctcg ggctcgacac cggggagcag gccgatgccg cgcacgctca ccttgcgcag
      661 cacgttgccg aaggcgccgg ccacgagcac ctcggccatc tcgtcgagcg ccagccccag
      721 cttgcggcaa gccaaccggg ccgcggtcag gatggccccc ttggcgagct ggatctcccg
      781 gacgtcccgc gccgtgcaaa ccacctcgcg ggccgcgccc tcgtccgggc tcgcgaggac
      841 gaacgcctgc tggccgtcga tctgccgcag ccggctccca tagccagccg ccgacgccgg
      901 cagctcgctc gccttccgca tggccccaga cggggtgagg atgcccacgc ggagcagttc
      961 ggcaaccgca tcgacgatgc cggaaccgca caggccgttc gccgcgacgc cgccgatgac
     1021 gttcaccagc acctgcccgt cccccggcag cgagacgagg tcgatcgccc ccggggcggc
     1081 ccgcatgccg catcgaatcg agaccccctc gaaggccggc cctgctgctg cggaggtgac
     1141 gacggcgcgc tcgcccgaaa ccgcgagcag ctcggtgttg gtgccgaggt cgatcagcag
     1201 acgcggctct ccgggctggt cgatgtcaca cgcgattgcc gccgcgaccg catcgcctcc
     1261 gacgtggctg cggatcatcg ggaagacgaa gacgttggcg ttgggatgga tcgccaactc
     1321 cacctccgcc gccttccagc atgcctgccc ggcccacacg gcggcgtacg gggccatccc
     1381 cagcccgacg atcggcacgc cggcccaggc gtgcaacatc gtcgggttcc cggcaacggc
     1441 tgcaacgacg acgtcgcgga ggtcgcaccc gacctccgcg acaagatcct ttacgagccc
     1501 ggcaagcccg ccgcgaactg cgcccgcgag cgccgccagg tgctcgtcgc gctccatcgc
     1561 gtggcggatg cgggcgatga cgtccgagcc gaacgcaacc tgcgggttga gacgcgcacc
     1621 cgaggcccgg acgctgccgt cggagagatc gacgagtgcc gcggcgagcg acgtggtgcc
     1681 gatgtccacc gcgaggccga aaccgctccg aacctcatcc cgagaagccg cgacgagctc
     1741 gttaccctcg agcgtcatgg tgagcggctg cccggccgcc caggcccggc ccagctctgc
     1801 gagaacggcg ggcttagcgg ccagcggact ggcgaggcca caggccgaag acaggcggtc
     1861 gagcagtgca ccctcggaaa ggtcgttggg cgggaggacg acggccagac gctcgacgac
     1921 gggccgtgcc agcagctccg gttcgacggg atcaccgaac gacttgccgg cgagtgaacg
     1981 cgaaacggga ggcacctcga tggtggccgg cccgtccagg accaggcggc agccgagccg
     2041 ccaaccctcc tcgaggtcgc gcggcccgat ctgatcgcgg tcgtcctcgg tgatgggagc
     2101 cgggcccgac cgaacccgca cccggcagcg gccgcaggtc ccctgcgcgc cgcacgggtg
     2161 ctcgaggagg atcccggcgc gcgcggcggc gacgtcgagc ccgagcgggc ccgtcgcacg
     2221 acagacgctc tcgtccggaa gaaaacgaac gtcgaagggc tcgcggtcca ccccgacggc
     2281 ttgtatcacc cgggagttgg ggtgtcaacg ggagggggtg tggcgggtga tagaatccag
     2341 caggcgatcg tgtccgtgcc gggggtgcag gaggtgctga aggtgctgga ggtgctggtg
     2401 ccggaggtgc tgaaagcctg ccgcgccgga gccgcgcacc gctcggcgaa ggcgggtgcc
     2461 ggaggtgctg gtgccggagg tgctgaaggt tgccgcgccg tagccgcagc ctgcccgccg
     2521 tagccacgtc gcgagcggcg aaggcgggag cttagcgagc gttggcgaag gcggaccggc
     2581 tgttcgctgt cagctgtcgg ctgccagctg ttcaggaaat gcacgagctg tccatcgcgg
     2641 aatcgatcgc cgactcggtg cggcggcacc ctgcgctgca aggcggacgc aaagtgaggc
     2701 gaatcggcgt ccaggtgggc gacacctcgg gcgtcaatgc cgaggcgctg gacttctgct
     2761 tcacaatcgt ggtccgcggg accgagctcg acggtgcggc gctcgacctc gagcgcgtgc
     2821 ccgtccggtt ccgctgcgag acctgcggcc acgagttcga gccggtggag ttcgacccgc
     2881 gatgcccggc gtgcggcgcc gaacgcgggc gcctgtccgg cggcgacgag ctgtcgctcg
     2941 cctacctcga gctcgacgac gaggaaccag gggagacgag ggagtgaccg agcgggtgac
     3001 ggtgcgggag aacgtgctca aggagaacga ccggattgcc gccggcctgc gggcggcttt
     3061 cgaccgcggc gcgatcttct gcgtgaacct gatctcctcg cccggcgccg gcaagaccag
     3121 catcctggaa ggcacgctcg aggcgctgca gggcacggcc gtgcgcgccg ccgtcctgac
     3181 gggcgacatc cagaccgagc gggatgccga ccgcctgcgg cgccacgggt tcccggcgct
     3241 tcagatcacg acgggcggca cctgccacct cgacgccgcg atggtgcggc gggcgctcga
     3301 cagcctctct ctcgacgcga tggacgtgct gttcatcgag aacgtcggga acctcgtgtg
     3361 ccccgcatcc tacgacctgg gcgaggacgt caaggtggtc gtgctcagcg tcaccgaggg
     3421 cgacgacaag ccgctcaagt accccgggat ctttcgccgt gccgggctga tgctgctgca
     3481 caagatggac ctgctgccgc acgcacgctt cgaccccgcg gcggccgtcg ccgacgcccg
     3541 gcgggtgcag ccggacctcg aggtggtgga aaccagctgt tacggcgccc ccgggctcga
     3601 ggcgtgggtc gggtgggttc ggagccgcct cgacgcgaag cgcgcagcgg gtcgcaccct
     3661 gagcgctgag ccctaagccc gagccgtctc tgtaaccgcc cccgcgttgc gtgaatcatc
     3721 cggtggaggg gccgcaggcc ccgtgagg
//
LOCUS       DAOPQX010000365         3738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26717_length_3738_cov_5.406462, whole genome shotgun sequence.
ACCESSION   DAOPQX010000365 DAOPQX010000000
VERSION     DAOPQX010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26717_length_3738_cov_5.406462"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1729)
                     /gene="mutS"
                     /locus_tag="PKK95_15100"
     CDS             complement(<1..1729)
                     /gene="mutS"
                     /locus_tag="PKK95_15100"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOC19594.1"
                     /translation="MSSPADSRSSTPAAAATPAMRQFLDAKRQYPDAIVFFRMGDFYE
                     MFYEDALVAARALELTLTSRAKDSNGGAIPMCGVPYHAVDGYLARLVRKGFRVAICEQ
                     VEDPRKAKGLVRREVVRVVSPGTLTDAAYLEAREPAFLMALAPVAAAPPRPSPRTADP
                     AAVSLPIPADPRPDGVQGASPAAARLVGVGAALVDLSTGEFTAAEYSGPAGLQALADE
                     LGVLRPREIVIPDDFALDAQLPAVASAGIPTTRVEAWTFEAERARAALLEQLRTASLE
                     GFGLEGRPLAVRAAGGLVRYLRDGQKADLAHVREIAFRQAADALVVDAVTLRHLEVVE
                     ASTGGRAGSLLDEIDRTVTAMGGRMLRAWLLRPLLSLERVRDRLDAVEDFAFHTTERA
                     KFREALKAVQDLERLVARVALGSAGPRDLVGLKVSLGAVPRLRHVLSGLQAPLAASLA
                     AALDDVAEVRSLVEAAVVDDPPALAREGGFVRDGFDAELDEARAISRSGKQIIARMED
                     EERGRTGIASLKVRYNRVFGYYIEVSKANLHAVPADYQRKQTIAGGERFVTPALKEYE
                     EKVLGAEERI"
     gene            1758..2525
                     /gene="tsaB"
                     /locus_tag="PKK95_15105"
     CDS             1758..2525
                     /gene="tsaB"
                     /locus_tag="PKK95_15105"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOC19595.1"
                     /translation="MTRAASMLVLALDTTTRAGSLALARDDRLLEAWSGDPDLTHASR
                     LPGDILDILRRHGLALADVDLYGVAAGPGSFTGLRIGIATVQGLAFAQGRKVVGVSAL
                     EALAWSAGEPAGSTSLVAAWMDARRGEVFASLGTPGEPVSLAGPEVASPGEVLGRWDG
                     LVGRRPVVWVGDGALAYRALIEQGARGRAWAVLPEVPMLAAAIAAIARRRASAGEATP
                     PHGVRPLYVRRPDAELARDRKRAATGAAEAGRREDRR"
     gene            2522..3064
                     /gene="rimI"
                     /locus_tag="PKK95_15110"
     CDS             2522..3064
                     /gene="rimI"
                     /locus_tag="PKK95_15110"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOC19596.1"
                     /translation="MSQRPGVPEPQPPWAAEVLPPGWRVETLSEGDLDEVLEIERASF
                     SNPWSRDMFLWELQNAGISYGYVLREPGGRVAAFCTVWIVQDELHVNNVAVGPAHRGK
                     GVGQALLRFVLRLAAGLGARRATLEVRRSNVPALRLYERLGFRAVGVRRNYYSSPVED
                     ALILWCDLPADEVKLRPDTA"
     gene            3201..3458
                     /locus_tag="PKK95_15115"
     CDS             3201..3458
                     /locus_tag="PKK95_15115"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOC19597.1"
                     /translation="MTADSQDLKPLLLETDEEFRQLAKKHHELEERLHVLTSKHYLSD
                     PEQVEEITLKKRKLQLKDRMEAIVRRYRDRPPLQSPSLQQG"
     gene            3529..>3738
                     /locus_tag="PKK95_15120"
     CDS             3529..>3738
                     /locus_tag="PKK95_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19598.1"
                     /translation="MKIDRAGYPFIAGALLPALILTALRRPRLGLPFAALAAALTFFF
                     RDPERRSPREADVVLVNTCAFIESAK"
BASE COUNT          531 a         1362 c         1321 g          524 t
ORIGIN      
        1 ggatgcgctc ctcggcgccg aggaccttct cctcgtactc cttcaacgcc ggggtcacga
       61 agcgctcgcc gccagcgatc gtctgcttgc gctggtagtc ggccgggacc gcgtgcaggt
      121 tcgccttcga cacctcgatg tagtagccga acacccggtt gtagcgcacc ttgagcgagg
      181 cgatcccggt gcgcccgcgc tcctcgtcct ccatgcgggc gatgatctgc ttgccgctgc
      241 ggctgatggc ccgcgcctcg tccagctcgg cgtcgaagcc gtcgcgcacg aaaccgccct
      301 cgcgggccag ggccggcggg tcgtccacca cggcggcctc gaccagcgac cggacctcgg
      361 ccacgtcgtc cagcgcggcc gcgaggctcg cggccagcgg cgcctgcaag ccggagagca
      421 cgtgccgcag ccgggggacg gcaccgagcg acaccttcag cccgaccagg tcgcgggggc
      481 cggccgagcc gagggcaacc cgggcaacca ggcgctccag gtcctgcacc gccttcagcg
      541 cctcgcggaa cttcgcgcgc tcggtggtgt ggaaggcgaa gtcctcgacg gcgtcgaggc
      601 ggtcgcgcac gcgctcgagc gacaacagcg gccgcagcag ccaggcgcgg agcatccgtc
      661 cgcccatggc cgtcacggtc cggtcgatct cgtcgagcag cgagccggcg cgccccccgg
      721 tggacgcctc caccacctcg aggtgccgca gcgtgacggc gtccaccacc agcgcgtcgg
      781 cggcctggcg gaaggcaatc tcgcgcacgt gtgccaggtc ggccttctgg ccatcccgga
      841 ggtagcgaac caggccgccc gcggcccgca ccgccagcgg ccgcccctcg aggccgaagc
      901 cctcgaggct tgccgtccga agctgctcga gcagcgccgc ccgggccctc tcggcctcga
      961 aggtccaggc ctccactctc gtcgtgggga tcccggccga ggcgacggcc ggcagctggg
     1021 cgtcgagagc gaagtcgtcg gggatcacga tctcgcgcgg ccgcaacacg ccgagctcgt
     1081 cggccagcgc ctgcagaccc gcgggccccg agtactcggc ggccgtgaac tcgccggtgg
     1141 agaggtctac cagcgcggcc cccacaccca ccagccgcgc cgccgcgggc gaggcgcctt
     1201 gcacaccgtc ggggcggggg tcggccggga ttggcagcga cacggccgca gggtcggccg
     1261 tgcgcggcga cgggcgcggg ggtgccgcgg cgaccggcgc cagcgccatc agaaaggccg
     1321 gctcgcgcgc ctcgagatac gccgcgtcgg tcagtgtgcc gggcgagacg acgcgcacca
     1381 cctcgcgccg caccagcccc ttggccttcc tggggtcctc gacctgctcg caaatcgcga
     1441 cccggaaccc ctttcgcacc aggcgggcca ggtagccgtc caccgcgtga tagggcacgc
     1501 cgcacatggg gatggcgccg ccgttcgaat ccttcgcccg ggaggtgagg gtcagctcga
     1561 gcgcgcgggc ggccaccagg gcgtcctcgt agaacatctc gtagaagtcg cccatgcgga
     1621 agaagacgat cgcgtcgggg tactggcgct tcgcgtcgag aaactgccgc atcgccggcg
     1681 tcgcggcggc cgcgggagtg gacgatctgg aatcggcagg tgacgacatg cgggctcggg
     1741 cttcgagaca gtctacaatg acgcgagccg catccatgct cgtgctcgcg ctcgacacca
     1801 ccacccgcgc cggcagcctc gctttggcgc gcgacgaccg gctgctcgag gcctggtcgg
     1861 gcgacccgga cctcacccac gcgtcccgcc tcccgggcga catcctcgac atcctgcggc
     1921 gtcacgggct ggcgctcgcc gacgtcgatc tctatggggt cgccgctggc cccggctcgt
     1981 ttaccggcct gcgcatcggc attgcgacgg tccaggggct ggccttcgcg cagggccgaa
     2041 aggtggtcgg cgtctcggcg ctcgaggcgc tggcctggtc ggcgggtgag ccggctggat
     2101 ccacctcgct cgtggccgcc tggatggatg cgcggagagg cgaggtgttc gccagcctcg
     2161 gcaccccggg agagcccgtc tcgctggcgg gccccgaggt cgcatccccc ggcgaagtgc
     2221 tcggccgatg ggacgggctg gtcggcaggc ggccggtcgt gtgggtgggg gacggagcgc
     2281 tggcgtaccg cgctctcatc gagcagggag cgcgcggacg cgcctgggcg gtgctgcccg
     2341 aggtcccaat gctggcagcc gccatcgcgg cgatcgcgcg ccggcgcgcg tcggcgggag
     2401 aggccacgcc gccgcacggc gtccgtccgc tctacgtccg ccggcccgat gccgagctgg
     2461 cccgtgaccg aaaacgagcg gccaccggcg cggccgaggc cggccgccgc gaggaccgcc
     2521 ggtgagccag cggcccggcg ttcccgagcc acaacccccg tgggcggccg aggtgctgcc
     2581 gccgggctgg cgcgtcgaga ccctgtcgga gggcgacctc gacgaggtgc tggagatcga
     2641 gcgcgcctcg ttcagcaacc cctggtcgcg cgacatgttc ctctgggagc ttcagaacgc
     2701 cggcatctcg tacggctacg tgctccgcga gcccggcgga cgcgtggccg ccttctgcac
     2761 cgtgtggatc gtgcaggacg agttgcacgt gaacaacgtg gccgtcggcc cggcgcatcg
     2821 cgggaaagga gtcggccagg cgttgctgcg gttcgtgctg aggctggcgg ctggtctcgg
     2881 cgcgcggcgc gcaaccctcg aggtcaggcg gtcgaacgtc ccggcgctgc ggctctacga
     2941 gaggctggga ttccgcgccg tcggcgtgcg ccggaactac tactcgtcgc ccgtcgagga
     3001 cgcgctgatc ctctggtgcg acctgcccgc cgacgaggtg aaactccgac cggatacggc
     3061 ttgaatcggc cctgtcgtta tggtaccttc cggctaggat gtgtgtcggt gctcacccga
     3121 ccggtccccg cctgttcgac tcgaatcatc ttccgcgggt gcagccgcgt gctcagtcac
     3181 agcctggcaa ggaggggaga atgacggcag actcacagga tttgaagccc ctgctgctcg
     3241 aaaccgatga ggagttccgt caactcgcga agaagcatca cgagctcgaa gagcgcctgc
     3301 acgtgctcac gagcaagcac tacctgtcgg atcccgagca agtcgaggaa atcacgctca
     3361 agaaacgcaa gctccagctg aaggaccgga tggaagcgat cgtgcgccgg taccgggatc
     3421 gtcccccact ccagtccccg agcctgcagc aggggtgatc gcagccgata agggccgggc
     3481 accgtcgtcc gaccaggggg gcgagacccg gcccgttttc ctctctccat gaagatcgat
     3541 cgcgcggggt acccgttcat cgccggcgcc ctgctgcccg cactcattct cacggccctc
     3601 agacgcccgc gcctcggcct gccgttcgcc gccctcgccg ccgcgctgac cttcttcttc
     3661 cgcgatcccg agcgccgctc tccgcgcgag gccgacgtgg tgctggtgaa cacctgcgcc
     3721 tttattgaaa gcgccaaa
//
LOCUS       DAOPQX010000366         3732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26781_length_3732_cov_3.865923, whole genome shotgun sequence.
ACCESSION   DAOPQX010000366 DAOPQX010000000
VERSION     DAOPQX010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26781_length_3732_cov_3.865923"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..2361)
                     /locus_tag="PKK95_15125"
     CDS             complement(70..2361)
                     /locus_tag="PKK95_15125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOC19599.1"
                     /translation="MSDEKKIDRRAFIVEGSAAVVGVTVAGGARAGAQPAKPAKPSSF
                     SSPAGSVIPYSREELMRRAPARVFGGRALGEIAFPLGGIGTGTVSLGGRGELRDWEIF
                     NRPNKGRSLPFTFVALWARQPGKAPVVQVVEAPLQPPFRGSHGYAREGAQGLPRFRGA
                     TFTGAYPFAEVAFEDADLPVQVGLTAFNPMIPLEVGDSSLPVAIFRYSLTNRSASPVD
                     LALAFSILNPIGYDGHSPLGGNRFAGFGQNVTRVRRDASIVGLDLTSQKYEPDDILAG
                     SMALVTTHPEVSARPAWEEGAWWDSFQKWFDEYAADGTFDQDGSAGPSADGVSQHATL
                     APRFSLAPGESKAIVFLLAWHFPLRENYWNREPEVRGRKLRNDYGARFKDAWEVARHA
                     AANLERLEQGTRSFRRALEEGSLPAAVLDAVSSQMSIIRTNTCMLLEGQRFFAFEGTS
                     DDSGCCPLNCTHVWNYEQALAHLYPSLERSMRRTDFLDNLRPDGSMAFRTLVPTGRAL
                     WQFKPAADGQMGCVLKLYREWQLSGDEAFLRELWPHAKRALEYAWLHWDADRDGVMEG
                     EQHNTYDIEFYGPNTMMGTLYLGALLAGERMARAVGEEAAAAEYRRVFESGRAKLDAE
                     LWRDGYYVQRVPDPSAVKVQEVEQKESWHASSVDRGSIKYQYGEGCLSDQLLGQWLAT
                     VVGLGHVLPPGHVRETLQSIYRHNFRHDFFDHPNTQRLYALNDEKGLVLCSWPRGHRP
                     ELPFVYSDEVWTGIEYQVAAHLI"
     gene            complement(2419..3576)
                     /locus_tag="PKK95_15130"
     CDS             complement(2419..3576)
                     /locus_tag="PKK95_15130"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOC19600.1"
                     /translation="MNRRFVWLVPVLAAGCAVAQLAAQKPASSHLATGLGDLSRLSRA
                     QSRSISPENFTGEKGKGGMATEGTGANAARDLGQGWKVSPSVKIKPGETFVMADIAGP
                     GVINHIWMTPTGTARFTIFRIYWDGETQPSVETPVGDFFASGWGGTYPRVNSLPICVN
                     PGSGLNSYWQMPFRKSAKITMENIDSREMTLYYQIDYSLADVPSDSGYFHAQFRRVSP
                     LPYKTDYTILDGVKGWGHYVGTYLAWGVHNTGWWGEGEIKFFIDGDNPFPTICGTGTE
                     DYFCGSYNFDNPDTRQYEPFNTPYVGLAQVIKPDGVYKSQQRFGMYRWHILDPIRFEK
                     DLRVTIQALGWRSDGRYLPLQDDIASVAYWYQAEPHAAFPALAGKDDLEVK"
BASE COUNT          570 a         1294 c         1289 g          579 t
ORIGIN      
        1 ccggcgctcg ccgtcgtagc ggtctcgcac ggccttggtg acggccagcc cctctgccac
       61 cagcccctcc tagatcaggt gcgccgccac ctggtactcg atgcccgtcc acacctcgtc
      121 ggaatagacg aacggcagct cgggacggtg cccccgcggc cagctgcaga ggaccagccc
      181 cttctcgtcg ttcagcgcgt agaggcgctg cgtgttgggg tggtcgaaga agtcgtggcg
      241 gaagttgtgc cggtagatgg actgcagcgt ctcgcgcacg tgcccgggcg gcagcacgtg
      301 cccgaggccc acgacggtgg ccagccactg cccgagcagc tgatccgaga ggcacccctc
      361 cccgtactga tacttgattg acccgcggtc caccgaggac gcgtgccacg attccttctg
      421 ctcgacctcc tgcaccttca cggccgacgg gtcggggacg cgctgcacgt agtagccgtc
      481 gcgccacagc tcggcgtcga gcttcgcccg tccgctctcg aagacgcggc ggtactcggc
      541 cgcggccgcc tcttctccca ccgcgcgggc catccgctcc cccgccagca gtgcgccgag
      601 gtagagcgtc cccatcatcg tgttcgggcc gtagaactcg atgtcgtagg tgttgtgctg
      661 ctccccctcc atcacgccgt cgcggtcggc gtcccagtgc agccacgcgt actcgagcgc
      721 ccgcttggcg tgcggccaca gctcgcgcag gaacgcctcg tcgcccgaca gctgccactc
      781 ccggtacagc ttcagcacgc accccatctg cccgtctgcc gccggcttga actgccagag
      841 cgcgcggccg gtcgggacga gggtgcggaa cgccatcgag ccgtccgggc gcaggttgtc
      901 gagaaagtcg gtccggcgca tggagcgctc gagcgacggg tacaggtgcg ccagcgcctg
      961 ctcgtagttc cacacgtgcg tgcagttcag cgggcagcac ccgctgtcgt cgctggtccc
     1021 ctcgaacgcg aagaagcgct ggccctcgag cagcatgcag gtgttggtcc ggatgatcga
     1081 catctggctc gacacggcgt cgaggacggc ggccggcagg ctgccctcct cgagcgcccg
     1141 gcggaacgac cgcgtgccct gctcgaggcg ctcgaggttc gcggcggcgt gccgtgccac
     1201 ctcccacgcg tccttgaatc gcgcaccgta gtcgttccgc agcttgcgcc cgcggacctc
     1261 gggctcgcgg ttccagtagt tctcccgcag cgggaagtgc cacgccagga ggaagacgat
     1321 cgccttcgac tcgccgggcg cgagcgagaa gcgcggcgcg agcgtcgcgt gctgggagac
     1381 gccgtccgcg ctgggacccg cggacccgtc ctggtcgaag gttccgtcgg ccgcgtactc
     1441 gtcgaaccac ttctggaacg agtcccacca ggcgccctct tcccacgccg gccgggcgct
     1501 cacctcgggg tgcgtcgtga cgagcgccat cgaccccgcc aggatgtcgt cgggctcata
     1561 cttctgcgac gtcaggtcga gtccgacgat cgaagcgtcc cggcgcaccc tcgtgacgtt
     1621 ctgcccgaat cccgcgaagc ggttgccgcc aagcggcgag tggccgtcgt agccgatcgg
     1681 gttgaggatc gagaaggcga gagcgaggtc caccggggac gcggagcggt tggtgagcga
     1741 gtagcggaag atcgcgacgg gcagcgacga gtcgcccacc tcgaggggaa tcatagggtt
     1801 gaatgccgtc agcccgacct gcaccggcag gtcggcgtcc tcgaaggcca cctccgcgaa
     1861 gggataggcg ccggtgaagg tggccccccg gaaccggggc agcccctgcg ccccctcgcg
     1921 cgcgtagccg tgcgagccgc gaaacggcgg ctgcagcggc gcctcgacca cctgcacgac
     1981 cggggccttg cccggctggc gggcccagag cgcgacgaag gtgaacggca gcgagcgccc
     2041 cttgttgggc cggttgaaga tctcccagtc gcgcagctcg ccgcggccgc cgagcgacac
     2101 ggtgccggtg ccgatgccgc cgagcgggaa ggcaatctcg ccgagcgccc ggccgccgaa
     2161 gacccgcgcc ggcgcacgcc gcatcaactc ctcgcgcgag tacgggatca ccgacccggc
     2221 cggcgaggag aacgacgacg gcttggctgg cttggcgggt tgggcgccgg cgcgcgcgcc
     2281 gccggctacc gtaaccccca ccacggccgc cgacccctcg acgatgaacg cgcggcgatc
     2341 gatcttcttc tcgtctgaca ttgtcgtgac cccttcaacc ggcagtgcga cccgttgggg
     2401 cgaccacgag ggtcgcccct acttcacctc caggtcgtcc tttcccgcca gcgccgggaa
     2461 ggcggcgtgc ggctcggcct ggtaccaata ggccaccgat gcgatgtcgt cctgcagcgg
     2521 caggtagcgg ccgtccgacc gccagccgag cgcctggatc gtcacccgca ggtctttctc
     2581 gaagcggatc gggtcgagga tgtgccagcg gtacatgccg aaccgctgct gcgacttgta
     2641 caccccatcg ggcttgatga cctgcgcgag gccgacgtaa ggcgtgttga acggctcgta
     2701 ctggcgcgtg tcggggttgt cgaagttgta ggagccgcag aagtaatcct cggtgccggt
     2761 tccgcagatc gtcgggaacg ggttgtcgcc gtcgatgaag aacttgatct cgccctcgcc
     2821 ccaccagccg gtgttgtgga cgccccacgc gaggtaggtg ccgacgtaat gcccccagcc
     2881 cttcacgccg tccaggatcg tgtagtcggt cttgtagggc agcgggctga cgcgacggaa
     2941 ctgggcgtgg aagtacccgg agtcggacgg cacgtcggcc agcgagtagt cgatctggta
     3001 gtacagcgtc atctcgcggc tgtcgatgtt ctccatcgtg atcttcgcgg acttgcggaa
     3061 cggcatctgc cagtacgagt tcaggccgct ccccggattc acgcagatcg gcagggagtt
     3121 gacgcgcggg taggtgccgc cccagcccga ggcgaagaag tcgcccaccg gggtctcgac
     3181 cgacggctgc gtctcgccgt cccagtagat ccggaagatg gtgaagcggg cggtgccggt
     3241 cggcgtcatc cagatgtggt tgatcacgcc cgggcccgcg atgtcggcca tcacgaacgt
     3301 ctcgcccggc ttgatcttca ccgagggcga gaccttccac ccctggccga ggtcgcgcgc
     3361 cgcgttggcg ccggtcccct cggtcgccat cccccccttg cccttctccc cggtgaagtt
     3421 ctccgggctg atggatcgcg actgcgcgcg cgagagccgg gacaagtcgc caaggcccgt
     3481 cgcgagatgg gacgaggcgg gcttctgcgc tgccaactga gcgaccgcgc aacccgcggc
     3541 caggacgggg accagccaga cgaaacgtcg gttcatgtgg gctcctcctg cgcggcgcag
     3601 catagcacag ggaccgtacg ggaattcacc gcagagacgc ggggggaaca gagaagagat
     3661 cgtgcaggag gtgcagatgg tgcaggtggt gccggaggtg ctggtgcagg tggtgccgga
     3721 ggtgctggtg ca
//
LOCUS       DAOPQX010000367         3729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26816_length_3729_cov_4.062874, whole genome shotgun sequence.
ACCESSION   DAOPQX010000367 DAOPQX010000000
VERSION     DAOPQX010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26816_length_3729_cov_4.062874"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /gene="nuoF"
                     /locus_tag="PKK95_15135"
     CDS             complement(<1..1271)
                     /gene="nuoF"
                     /locus_tag="PKK95_15135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOC19601.1"
                     /translation="MNPVLTPHVREPNGHTLEAYLRRGGYGGLRKALGMPPGEVIEMV
                     KASGLRGRGGAGFPTGMKWQFVPKDSPKPKYLCCNADESEPGTFKDHVLMERNPHLLL
                     EGCAIGCHAIGAKVAYIYIRGEFYHVQRVLEAAIEEARAAGYLGHDIFGSGFDCEIYV
                     HRGAGAYEAGEESALLESLEGKRAQPRIRPPFPAVVGLYGCPTAINNVETLCNVPLIL
                     TRGPEWFASLGPEKNGGPKLYCISGHVEKPGVYEASMHVTLRELIYGHAGGIRGGRAL
                     KAVIPGGSSTPVILPDRIDAQASFDGLLKAGSMLGSAAIIVMDDTTCMVWLAENLLHF
                     YRHESCGKCTPCREGTDWLVKILHRIEAGEGSMRDIDLLLGVSANIGGKTLCPFGDAA
                     IAPVVSTIQHFRHEYEAHVREGRCTCPAEWR"
     gene            complement(1349..1990)
                     /gene="nuoE"
                     /locus_tag="PKK95_15140"
     CDS             complement(1349..1990)
                     /gene="nuoE"
                     /locus_tag="PKK95_15140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOC19602.1"
                     /translation="MTFHPEMRYEEGLHVPRRELRDEGEPFAFTPENRQRLEAIASHY
                     PPEHRRSAVIPALYLVQDQQGYVTASAIRHVAEAIGCTEAEVDDVVTYYAMFFRKPVG
                     RYVIQVCRTLSCALRGAERVTAELERALGVEVGQTDATGTFTLMEMECLGACDRAPVV
                     MVNNELWHECQSPDQVRALLDGLREQGPDALTGCHLDVEKKQGQAPDERETGN"
     gene            complement(1987..3168)
                     /gene="nuoD"
                     /locus_tag="PKK95_15145"
     CDS             complement(1987..3168)
                     /gene="nuoD"
                     /locus_tag="PKK95_15145"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOC19603.1"
                     /translation="MAELRTETMTVNMGPQHPSTHGVLRLVLQLDGETVVSVQPTIGY
                     LHTGIEKTAEEKKYQQVIPLVERMDYLSSHSNSLGFALSVEKLLGLEMPRRVQDIRLL
                     LAELQRLNSHLVWLGTHGMELGAVSIMLYTFRERELLLDVNELIAGFRMFPSYIRVGG
                     LREDLPPGFHESVARILDLLPGKIEEYEDLLTHNEIFRKRTRGVGVISGASALSYGLV
                     GPIARGAGVPYDVRKAFPYCGYETYQFDVPVGTRGDVYDRYLVRIEEMRQSIRICRQA
                     LERIPPKGEWAVDDPRVVPPPKDRVYTEMEALIQHFLLYSQGFLVPRGEAYVPVEGPR
                     GEHGCYVVSDGGNRPWRIKMRSPSMLACQALHEMIVGGMIADVIAVIGSIDVVMGDVD
                     R"
     gene            complement(3168..>3729)
                     /locus_tag="PKK95_15150"
     CDS             complement(3168..>3729)
                     /locus_tag="PKK95_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC19604.1"
                     /translation="FIAAISAALPGAGLEDGQGVDQPTLVVPRDRLVEVCEALRDQPA
                     LRFDVLADMTAVDWWPREPRYTVVYHLLSTVNRLRLRLEVALDGADAHVPTVRRTWAS
                     AGWLEREVWDLFGIVFDGHGDLRRLLMPEDWEGHPLRKDYPVQISMKPKVYAPLQVTE
                     EEFHSQIEKDRTVRGGTGLERGRERS"
BASE COUNT          543 a         1317 c         1249 g          620 t
ORIGIN      
        1 tccctccatt cggccgggca cgtgcagcgc ccctcgcgca cgtgcgcctc gtactcgtgg
       61 cggaagtgct ggatggtgct caccaccggc gcgatcgcgg cgtcgccgaa cgggcacagc
      121 gtcttgccgc cgatgttcgc cgagacgccg agcagcaggt cgatgtcgcg catcgaccct
      181 tcgcccgcct cgatccggtg gaggatcttc accagccagt cggtcccctc tcggcagggg
      241 gtgcacttcc cgcacgactc gtggcggtag aagtggagca ggttctcggc cagccagacc
      301 atgcaggtcg tgtcgtccat cacgatgatc gccgccgacc cgagcatcga ccctgccttc
      361 agcaggccgt cgaagctggc ctgggcgtcg atccggtcgg gcaggatcac cggcgtcgac
      421 gaaccgcccg ggatcaccgc cttcagcgcc cgtccccccc ggatcccgcc ggcgtgcccg
      481 tagatcagct cgcgcagcgt cacgtgcatc gacgcctcgt acacgcccgg cttctcgacg
      541 tggccgctga tgcagtacag cttcggcccg ccgttcttct cgggcccgag cgaggcaaac
      601 cactccggcc cgcgcgtcag gatcagcgga acgttgcaga gggtctcgac gttgttgatg
      661 gcggtcgggc agccgtacag cccgacgacg gccgggaagg gcggccggat gcggggctgc
      721 gcgcgcttcc cctcgagcga ttcgagcagc gcgctctcct cgcccgcctc gtacgcgccg
      781 gcgcccctgt ggacgtagat ctcgcagtcg aagccgctcc cgaagatgtc gtggcccagg
      841 tagcccgcag cccgcgcctc ctcgatggcc gcctcgagca cacgctgcac gtgatagaac
      901 tcgccgcgga tgtagatgta ggcgaccttg gcgccgatcg cgtggcagcc gatggcgcac
      961 ccctcgagca gcaggtgcgg gttgcgctcc atcagcacgt ggtccttgaa ggtgcccggc
     1021 tcgctctcgt cggcgttgca gcagaggtac ttcggcttgg gcgagtcctt cgggacgaac
     1081 tgccacttca tccctgtagg gaaccccgcg ccccctcggc cgcgcagccc cgacgccttc
     1141 accatctcga tgacctcgcc gggcggcatg ccgagcgcct tgcgcaggcc gccgtatcct
     1201 ccgcggcgga ggtacgcctc gagcgtgtgg ccgttgggtt cgcggacgtg cggggtgagg
     1261 acgggattca tgcgaccgta agttcttagt tcttagttct tagttcttag ttcttagttc
     1321 ttagttctta gttcttagtt gctggttgtt agttgccggt ttcgcgttcg tcaggagcct
     1381 gcccttgctt cttctcgacg tccagatggc agcccgtgag cgcgtcgggc ccctgctcgc
     1441 gcaggccgtc cagcagcgcg cgcacctggt cgggcgactg gcactcgtgc cacagctcgt
     1501 tgttcaccat cacgaccggc gcgcggtcgc atgcccccag gcactccatc tccatcagcg
     1561 tgaaggtccc ggtcgcgtcg gtctgcccca cctcgacgcc gagcgcccgc tccagctcgg
     1621 cggtgacacg ctcggcgccc cgcagggcgc acgagagggt gcggcacacc tgaatgacgt
     1681 accgcccaac cggcttgcgg aagaacatcg cgtagtaggt caccacgtcg tccacctcgg
     1741 cctcggtgca gccgatcgcc tcggcgacgt gccggatggc ggacgccgtg acgtagccct
     1801 gctggtcctg cacgaggtag agcgcgggga tgacggccga gcggcggtgc tcgggcggat
     1861 agtgcgacgc gatggcctcg agccgctgcc ggttctcggg ggtgaaggcg aagggctctc
     1921 cctcgtcgcg cagctcgcgt cgcgggacgt gcagcccctc ctcgtagcgc atctccgggt
     1981 ggaaggtcac cggtccacgt cccccatgac gacgtcgatc gagccgatga cggcgatcac
     2041 gtcggcgatc atgccgccca cgatcatttc gtggagtgcc tggcaggcca gcatcgaggg
     2101 cgagcgcatc ttgatgcgcc acggccggtt gcccccgtcc gacaccacgt agcagccgtg
     2161 ctcgccgcgc ggcccctcga ccggcacgta ggcttcgccg cgtgggacga ggaagccctg
     2221 cgagtagagc aggaagtgct ggatcagcgc ctccatctcg gtgtagacgc gatccttggg
     2281 cggcggcacc acgcgcggat cgtcgaccgc ccactccccc ttcggcggaa tccgctccag
     2341 cgcctgccgg cagatccgga tcgactggcg catctcctcg atccgaacca ggtagcggtc
     2401 gtacacgtcg ccgcgcgtcc cgaccgggac gtcgaactgg taggtctcgt acccgcagta
     2461 ggggaacgcc ttgcgcacat cgtagggaac gccggcaccc cgtgcgatcg gcccgaccag
     2521 cccgtacgag agggcgctgg ccccgctgat caccccgacg ccgcgcgtgc gcttgcgaaa
     2581 gatctcgttg tgcgtgagga ggtcctcgta ctcctcgatc ttcccgggca gcaggtcgag
     2641 gatgcgggcc accgactcgt ggaagcccgg cggcaggtcc tcgcgcaggc cgccgacccg
     2701 gatgtagctc gggaacatcc ggaagccggc gatgagctcg ttgacgtcca gcagcagctc
     2761 gcgctcccgg aacgtgtaga gcatgatcga gacggcgccc agctccatcc cgtgcgtgcc
     2821 gagccagacc aggtggctgt tcaggcgctg cagctcggcg agcagcaggc gaatgtcctg
     2881 cacccgccgc ggcatctcga ggccgagcag cttctcgacc gagagcgcga agccgaggct
     2941 gttggagtgc gagctgaggt agtccatccg ctcgacgagc gggatcacct gctggtactt
     3001 cttctcctcg gccgtcttct cgattcccgt gtgcaggtac ccgatggtgg gctgcaccga
     3061 cacgacggtc tcgccgtcga gctggagcac caggcgcagc accccgtgcg tgctggggtg
     3121 ctggggcccc atgttcacgg tcatcgtctc ggttcgcagc tcggccatca ggaacgctcg
     3181 cgcccccgct cgagcccggt gccgccccgg accgtgcggt ccttctcgat ctggctgtgg
     3241 aactcctctt cggtcacctg cagcggggcg tagactttcg gcttcatcga gatctgcacc
     3301 gggtagtcct tgcgcagcgg gtggccctcc cagtcttcgg gcatcagcag gcgccgcagg
     3361 tcgccatggc cgtcgaagac gatgccgaac aggtcccaca cctcgcgctc gagccagccg
     3421 gccgaggccc acgtgcggcg caccgtcggc acgtgcgcgt cggccccgtc gagcgccacc
     3481 tcgaggcgca ggcgcagccg gttcaccgtc gagagcaggt ggtagacgac cgtgtaccgc
     3541 ggctcgcgcg gccaccagtc caccgcggtc atgtcggcga gcacgtcgaa gcgcagcgcg
     3601 ggctggtcgc ggagggcctc gcagacctcg acgagccggt cgcggggcac gacgagcgtg
     3661 ggctgatcga caccctgccc gtcttcgagg ccggcgccgg gaagtgcggc cgagatcgca
     3721 gcgatgaag
//
LOCUS       DAOPQX010000368         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_26838_length_3727_cov_3.456427, whole genome shotgun sequence.
ACCESSION   DAOPQX010000368 DAOPQX010000000
VERSION     DAOPQX010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26838_length_3727_cov_3.456427"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PKK95_15155"
     CDS             <1..1012
                     /locus_tag="PKK95_15155"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOC19605.1"
                     /translation="TYPGFYHHRQVVVRGEATADDRGHARLAPRGGVERGIWLRITDG
                     VAPQGMVEVRGHFWDVGRFAPDDPQVTGINLPAWVAERIGDRWPAQGEMLVLTVTGVV
                     EPTPPSSMPTLRQLALDPERYAGQALTIAGLFGGRNLYGDLARAPRAGRHEFVLRAAG
                     GAVWVTGIEPRGRGWRLNPDARVDTGQWLEVTGTVRSGDGLVWVEGTKVDRTTARAQE
                     PPPPPPPALAPAPPPEVIFSVPSDEDTDVPPSSPVRIQFSRDLDPATLANRVVVTYLG
                     QEDAPIPFKARFDPTNRVLHLTFEQPFERYRIVRVELRAGILGTDGQKLEPYALTFTT
                     GG"
     gene            complement(1188..3290)
                     /locus_tag="PKK95_15160"
     CDS             complement(1188..3290)
                     /locus_tag="PKK95_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOC19606.1"
                     /translation="MNRRVLVIAGGCAVLAGAALVAQQQEQRPTFRAGAAYVRLDVYP
                     HEGDRPVVDLTRDDFEVFEDGVAQQIETFEHVVVRPGGPSATRIEPSSVGEARQMASD
                     PRARLFVLFLDTYHVDGTSGYQVRGPLLRLLNRAIGQDDLVAVMTPEMSGAAVTFTRR
                     TGAIEELLDRHWEYGRRFRRVGLDPDEELYEACYPPEPGDQAGTSGIAQEMSDRRREK
                     MTLDALEDLVIHLEGLRDERKAVLVISEGWPLYRPNSNLARTVRGRVPGRPQVGVGPD
                     GRLRSGDLGRSAGGHLLRCDQDRIRLAQEDHEQQFRRMLDLANRANATFYPVDPRGLP
                     VFDRPVGAPGVALSPNQDQMQLRGRLEALHTLASATDGIAVTNSNDIDAGLRRVVDDL
                     TSYYLIGYYSTNTRLDGRFRSVKVGVKRPGVTVRTRRGYLAPTPEEVEARAGAEKEAA
                     SSARPEAAALAALARQRANAFVHLAAGYEWLPGSAGPRGAALWLVAELDAAAGRRDEQ
                     WREGAEVTLALSGGGAGASESRHTLSPTSRALRLRFPDDDTGTLEPGEYTVRVTSKPV
                     GGTLSTSEVVRVTVPPLPEAGRTAVGNARLLRRGPFSGAGWVPTGDLRFRRQERVRVE
                     VPVAGTISESTTRLLDRNGKPLNVPVAPLSGGGTSAVAGGEVTLAPLAASDYLLETTI
                     KHPGGTEKVLTAFKIIAG"
     gene            complement(3287..>3727)
                     /locus_tag="PKK95_15165"
     CDS             complement(3287..>3727)
                     /locus_tag="PKK95_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOC19607.1"
                     /translation="LRLLFKREFEKVPILGPACGLAGFIPVERHNPAQSQAAVDAAAI
                     ELSRGHSFLVFPEGTRSRTGDLLPFKKGSFIMAIKAQVPVVPVAIRGGRSAMAKGSAI
                     IRPSLVDVRIGRPVETTGLTFDHRDELVERVRTGIEALRRGVDQ"
BASE COUNT          533 a         1340 c         1316 g          538 t
ORIGIN      
        1 gacatacccc ggcttctacc accaccgcca ggtggtcgta cgcggcgagg cgaccgcgga
       61 cgacagaggc cacgcgcggc tggccccgcg cggcggcgtg gagcgcggca tctggcttcg
      121 gatcaccgac ggcgtggccc cgcaaggcat ggtcgaggtg cgcggccact tctgggacgt
      181 agggcgcttc gcccccgacg acccgcaggt caccggcatc aacctgccgg cgtgggtcgc
      241 cgagcggatc ggtgatcgct ggccggcgca gggtgagatg ctggtgctca ccgtgaccgg
      301 cgtcgtggag ccgacacctc ccagctcgat gcccaccctt cgccagctcg cgctcgaccc
      361 tgaacgctac gcgggtcagg ccctcaccat cgccgggctg ttcggcgggc gcaacctcta
      421 cggagacctg gcgcgtgcgc cacgcgcagg ccgtcacgag ttcgtccttc gggctgccgg
      481 aggcgccgtg tgggtgacgg gcatcgagcc gagagggcgc ggctggcggc tgaaccccga
      541 cgcgcgcgtg gacacgggac aatggctcga ggtgaccggc acagtgcgca gcggcgacgg
      601 gctggtctgg gtggagggca ccaaggtcga ccggacgacg gcccgggcgc aagaaccgcc
      661 gcctcctccg ccgccggcgc tcgccccggc cccaccgccc gaggtcatct tcagcgtgcc
      721 gtccgacgag gacaccgacg ttccaccctc ctcgcccgtg cggatccagt tctcgcgcga
      781 tctcgatccg gcgacgctcg cgaaccgcgt cgtcgtgacg tacctgggcc aggaggacgc
      841 gccaatcccc ttcaaggcgc gcttcgaccc gaccaaccgg gtgctgcacc tcaccttcga
      901 gcagccgttc gaacgctacc ggatagtacg cgtggagctg cgggcaggaa tcctcgggac
      961 cgacggccag aagctggagc cctacgcgct gaccttcacg acgggaggtt agtcagcctc
     1021 acgcgtgtca cgctgcactc atggtcccac gggatctagc cgcagagacg cagagagcac
     1081 agagacgtac gcggagaaga accctgatcg aaattcactg atcgcccttc tcggcggtcg
     1141 tctctgcgtt ctctgtgtct ctgcggtgaa ttcccgtatc gtgagattca gccggcgatg
     1201 atcttgaacg ccgtcagcac tttctccgtg cctcccgggt gcttgatcgt cgtctcgagg
     1261 aggtagtcgg acgcggcgag cggggcgagc gtgacttccc cgccggcgac cgcagatgtg
     1321 ccgccgccgg agagaggcgc caccggcacg ttcagcggct tgccgttgcg gtcgagcagc
     1381 cgggtcgtgc tctcgctgat tgtgccggca accggcacct cgaccctcac gcgttcctgc
     1441 cggcggaagc gaaggtcgcc cgttggaacc cagcccgccc ctgagaaggg cccccgccgc
     1501 agcagtctcg cgttccccac cgcagtgcgc cccgcttccg ggagcggagg caccgtaacg
     1561 cgcaccacct ccgaggtcga gagcgtcccg cccaccggtt tcgaggtgac gcgcacggtg
     1621 tactcccccg gctcgagcgt ccccgtgtcg tcgtcgggaa agcggaggcg caaggcgcgc
     1681 gaggttggcg agagggtgtg ccggctttcg ctggcgcccg cgccgccgcc cgagagcgcc
     1741 agcgtcacct cggctccctc cctccactgc tcgtcccgac ggccggccgc cgcgtcgagc
     1801 tcggccacca gccagagcgc ggccccgcgc ggccccgcac tgccgggcag ccactcgtag
     1861 ccggccgcca ggtgaacgaa ggcgttcgcg cgctgcctcg ccagcgcggc gagggcggct
     1921 gcctccggcc tggcgctcga cgccgcttcc ttctcggccc cggcccttgc ctcgacctcc
     1981 tcgggcgtcg gcgcgaggta cccgcgccgc gtgcgcacgg tcactcccgg acgcttcacg
     2041 ccaaccttga cggaccggaa gcgcccgtcg agacgggtgt tggtcgagta gtagccgatc
     2101 aggtagtagg aggtcaggtc gtcgacgacg cgccgcaggc cggcgtcgat gtcgttgcta
     2161 ttggtgaccg cgatcccgtc ggtcgccgaa gccagcgtgt gcagcgcctc gagccggccc
     2221 cggagctgca tctggtcctg gtttggcgag agggccacgc cgggggcgcc gaccggccgg
     2281 tcgaacaccg gcagcccgcg tggatcgacc gggtagaacg tcgcgttggc gcggttcgcc
     2341 aggtcgagca tccggcggaa ctgctgctcg tggtcctcct gggcgagccg gatgcgatcc
     2401 tggtcgcagc gcagcaggtg gccgccggcg gagcggccga gatcgcccga gcgcaggcgg
     2461 ccgtccgggc ccacgccaac ctgggggcgg ccggggacgc gtccgcgcac cgtgcgcgcc
     2521 aggttcgagt tcggccggta cagcggccac ccctcgctga tcacgagcac cgccttgcgc
     2581 tcgtcgcgaa ggccctcgag gtggatgacg aggtcttcga gcgcgtccag cgtcatcttc
     2641 tcgcggcggc gatcgctcat ctcctgggcg atccccgagg tgcccgcctg gtcgcccggc
     2701 tcgggcgggt agcaggcctc gtacagctcc tcgtcggggt cgagccccac gcgtcggaag
     2761 cgcctgccat actcccagtg acggtcgagc agctcctcga tggcacctgt ccggcgcgtg
     2821 aaggtgacgg cggcccccga catctcgggg gtcatcacgg ccaccaggtc gtcctggccg
     2881 atcgcgcggt tcagcaggcg cagcagcggg ccgcgcacct ggtacccgga cgtgccgtcc
     2941 acgtgatagg tgtcgaggaa cagcacgaac agccgcgccc gggggtcgga ggccatctgc
     3001 cgcgcctcgc cgaccgaaga tggctcgatg cgggtggccg aggggccgcc gggccggacg
     3061 acgacgtgtt cgaaggtttc gatctgctgg gcaacgccgt cctcgaacac ctcgaagtcg
     3121 tcgcgggtga ggtcaacgac cgggcggtcg ccctcgtgcg ggtagacgtc gagcctgacg
     3181 tacgcggcgc ccgcgcggaa ggtgggacgc tgctcctgct gctgcgccac gagcgcggcg
     3241 ccagccagca ccgcacagcc gcccgcgatg acgaggactc gtcggttcat tggtccaccc
     3301 cacggcgcag ggcctcgatc ccggtccgga cgcgctccac cagctcgtcg cggtggtcga
     3361 aggtgaggcc ggtggtctcg accggccggc caatccgcac gtccacgagc gaggggcgga
     3421 tgatcgcgct gcccttcgcc atcgcggaac ggccgccccg gatggccacg ggaaccaccg
     3481 gcacctgcgc cttgattgcc atgatgaagc tgcccttctt gaagggtagc aggtcgccgg
     3541 tccggctgcg cgtcccctcc ggaaagacca ggaaggagtg accccggctg agctcgattg
     3601 cggcggcatc cacagcggcc tgcgactggg cggggttgtg gcgctcgacg ggaatgaagc
     3661 cggccaggcc gcacgcgggg ccgaggatgg gcaccttctc gaactcgcgc ttgaagagca
     3721 gccgcag
//
LOCUS       DAOPQX010000369         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27074_length_3702_cov_4.715108, whole genome shotgun sequence.
ACCESSION   DAOPQX010000369 DAOPQX010000000
VERSION     DAOPQX010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27074_length_3702_cov_4.715108"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PKK95_15170"
     CDS             <1..1082
                     /locus_tag="PKK95_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19608.1"
                     /translation="AYTMAGGIWSVAYTDALQLGLVALGLLVAIPVVFDAVGGLATAW
                     AGYAAARPGGAGLLPPFTPTSAWTLPAITAWWDVSAMLMLGGIPWNCYFQRVLSCRTP
                     SAARWHSVLSGLLTIAFTVPPLLLGVAAAQHAWPPEVSAALEARPAETLPAVFRYLTP
                     AWIAVLGLGAIIGAVTSSFSASILSAGSMFAWNCCSRFLRPAPSAAGMRRLVRVAVLL
                     LGAGAAVLALEVQSVQALWFFTSDLVFVLLFPQLVAALFDRRVNRIGSITAFSISLAL
                     RFGGGEPLFGIAPLIPYPELLAPLLPGGPSAWYDAATGAILLPYKTVAAAAGVATLPI
                     VSRLTARWDPPRPLGRVQQDADATS"
     gene            1079..1786
                     /locus_tag="PKK95_15175"
     CDS             1079..1786
                     /locus_tag="PKK95_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19609.1"
                     /translation="MIAILFAWAVHLYTALGLLCAAGIAVLVVHGGDEAFRGAFLLMA
                     LATAIDATDGWLARRARVEEVLPQFDGRRLDDLIDFQTYTTLPLLLVWRAELLGPGNS
                     WWLLAPLLASAYGFSQKDAKTEDHSFLGFPSYWNIVAAYLYLLRLPPAWALAIVLVLA
                     LLTFVPVRYLYTTHRGPLSTATNVMGGAWALGILAILWWWNEVPRAWVALSLLFPAYY
                     MAASWLLTIRRHRKAGR"
     gene            1966..3321
                     /gene="dnaB"
                     /locus_tag="PKK95_15180"
     CDS             1966..3321
                     /gene="dnaB"
                     /locus_tag="PKK95_15180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOC19610.1"
                     /translation="MSDTAAAAVAVERTLPHNLDAERSVLGAILIQNDAYNLAVAVVR
                     PEDFYRDAHRRIFARVIDLAERGMAIDFVTLKEELTRSGELETVGGPAYVASLVDGVP
                     RATNVEYYARIVKEKAILRSLIVAGNQIIAEAYHAEQDAAEVLDRAEHAIFQIAEGRV
                     RTGFTSLYDIAQDSVEAIQRAHEQKQLITGVPTGFHDLDQLTSGLQRGDLVIVAARPS
                     MGKTSLALNIAQHVGTYAKKTVGIFSLEMSKEQLFLRMLSSEAQIDGHALRTGFLRRD
                     DWGRLTTALGVLGQAKVYIDDTPSIGVLEMRAKARRLASEHGLDLLVIDYIQLMQGRG
                     RFDNRTQELGAISRSLKGLAKELNVPIVTLSQLSRAPESRSDHRPQLSDLRESGALEQ
                     DADVVLLIYRDDVYNKEPGENENVAEIIVAKQRNGPTDTVKLAFIRERTRFENYDPRS
                     V"
     gene            3333..>3702
                     /locus_tag="PKK95_15185"
     CDS             3333..>3702
                     /locus_tag="PKK95_15185"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOC19611.1"
                     /translation="MIRCTFAHVDLGALESNYRAIEAFLAARTPAGRRPPAIVAVVKA
                     NAYGHGAGPVALALERAGARMVACADIEEGIALRQAGVHVPVLVFGALSVSDLDGLFE
                     YRLTPTCSTPGAGKALQAAAA"
BASE COUNT          484 a         1324 c         1279 g          615 t
ORIGIN      
        1 ctgcttacac gatggccggc ggcatctggt cggtggcgta cacggacgcc ttgcagctcg
       61 gcctcgtcgc gctggggctg ctggtggcga tcccggtcgt cttcgacgcg gtgggcgggc
      121 tcgccacggc gtgggccggg tacgcggcgg cccgtcccgg cggcgcgggg ctgctgccgc
      181 ccttcacgcc gacgagcgcg tggacgctgc ccgcgatcac cgcgtggtgg gacgtgagcg
      241 cgatgctgat gctcggcggg atcccgtgga actgctactt ccagcgggtg ctgtcgtgcc
      301 ggacaccgtc ggcggcgagg tggcactcgg tgctctcggg gctgctcacc atcgccttca
      361 cggtgccgcc gctgctgctc ggcgtcgcgg cggcgcagca cgcctggccg ccggaggtgt
      421 cggcggcgct cgaggcgcgc ccggccgaga cgctgccggc ggtcttccgc tacctcaccc
      481 cggcgtggat agccgtgctc ggcctggggg cgatcatcgg cgcggtcacc tcgagcttca
      541 gcgcctccat cctgtcggcc gggtcgatgt tcgcgtggaa ctgctgctcg cggttccttc
      601 ggccggcccc gtcggcggcc ggcatgcgcc ggctggtgcg ggtcgcggtg ctgctgctcg
      661 gtgcgggggc cgccgtcctc gcgctcgagg tccagagcgt ccaggcgctg tggttcttca
      721 cgagcgacct ggtgttcgtc ctgctgttcc cgcagttggt cgccgcgctg ttcgatcgcc
      781 gcgtcaaccg catcgggtcg atcaccgctt tctccatctc gctcgcgctg cggttcgggg
      841 gcggcgagcc gctgttcggt atcgcgccgc tgatcccgta cccggagctg ctggcgccgc
      901 tcctcccggg cggcccttcg gcgtggtacg atgccgcgac cggcgcgatc ctgctgccgt
      961 acaagaccgt ggcggcggcc gctggcgtcg cgacgctccc gatcgtctcg cggctgacgg
     1021 cgaggtggga cccgccgcgc cctctcggga gggtgcagca ggacgcggac gctacttcat
     1081 gatcgccatc ctcttcgcct gggccgtcca cctctacacc gcgctggggc tgttgtgcgc
     1141 ggcagggatc gcggtgctcg tggtgcacgg cggcgacgag gcgttccgcg gcgcgttcct
     1201 gctgatggcg cttgcgaccg ccatcgacgc gaccgacggg tggctggcgc ggcgcgcccg
     1261 cgtcgaggag gtgctgccgc agttcgacgg ccgcaggctc gacgacctga tcgatttcca
     1321 gacctatacg acgctgccgc tgctgctggt ctggcgcgcc gagctgctcg gcccgggcaa
     1381 ctcctggtgg ctcctcgcgc cgctgctcgc gagcgcgtac ggcttcagcc agaaggacgc
     1441 caagacggag gatcactcct tcctcggctt tccctcgtac tggaacatcg tcgccgcgta
     1501 cctgtacttg ctgcgcctgc cgccggcgtg ggcgcttgcc atcgtgctcg tgctcgcgct
     1561 cctcaccttc gtcccggtgc ggtatctcta cacgacccac cgcggtccac tgagcaccgc
     1621 gacgaacgtg atgggcggcg cctgggcgct gggcatcctg gcgatcctct ggtggtggaa
     1681 cgaggtgccg cgcgcctggg tcgccctctc gctgctgttt cccgcctact acatggccgc
     1741 atcctggctg ctgaccatcc gacgacaccg caaagccgga cgataggaag acgccgcccg
     1801 gccgccgaag gcggccgccg aagaccggcc gaagttcccg tgtgatcggt cttcgtgatc
     1861 tttgtgatct ttctggctta ttcgtgactc tcgtggtcca tcgtgcggca ggccgcgagt
     1921 tgcggtagac tggtgcaccg cttcccgcag tcctgctcca cgcccatgtc cgatactgct
     1981 gccgccgccg ttgccgtcga acgtaccttg ccgcacaacc tggacgccga gcgttcggtg
     2041 ctcggcgcga tcctgatcca gaacgacgcc tacaacctcg ccgtggcggt cgtgcggccc
     2101 gaggacttct accgcgacgc gcaccgccgc atcttcgccc gcgtgatcga cctcgccgag
     2161 cgggggatgg cgatcgactt cgtcacgctc aaggaggagc tgacgcgctc gggcgagctg
     2221 gagaccgtgg gcgggccggc ctacgtggcc tcgctggtgg acggggtgcc ccgggcgacc
     2281 aacgtcgagt actacgcgcg gatcgtcaag gagaaggcga ttctgcgcag cctgatcgtc
     2341 gccgggaacc agatcatcgc cgaggcgtac cacgccgagc aggacgccgc cgaggtgctc
     2401 gaccgcgccg agcacgcgat cttccagatt gccgagggtc gcgtccgcac cgggttcacc
     2461 tcgctttacg acatcgcgca ggacagcgtc gaggcgattc agagggccca cgagcagaag
     2521 cagctgatca ccggcgtccc gacggggttc cacgacctcg accagctgac ctccggcctg
     2581 cagcggggcg acctcgtcat cgttgccgcg cggccgtcga tggggaagac cagcctcgcc
     2641 ctcaacatcg cgcagcacgt cgggacgtac gcgaagaaga cggtcgggat cttcagcctc
     2701 gagatgtcga aagagcagct gttcctccgc atgctctcct ccgaggcgca gatcgacggc
     2761 cacgccctgc gcaccgggtt cctgcgccgc gacgactggg gccggctgac caccgccctt
     2821 ggcgtgctgg gccaggccaa ggtctacatc gacgacaccc cgtcgatcgg cgtgctggag
     2881 atgcgcgcca aggcgcggcg cctcgcgtcc gagcacggcc tcgacctgct cgtgatcgat
     2941 tacatccagc tgatgcaggg gcgcggccgc ttcgacaacc gaacccagga gctgggggcg
     3001 atttcccgct cgctgaaagg gctggccaag gagctgaacg tcccgatcgt cacgctctcg
     3061 cagttgagcc gcgccccgga gtcgcgctcc gaccaccgcc cgcagctctc cgacctccgc
     3121 gagtcgggcg ccctcgagca ggacgccgac gtcgtcctcc tcatttaccg ggacgacgtg
     3181 tacaacaagg agcccggaga gaacgagaac gtcgcggaga tcatcgtggc caagcagcgc
     3241 aacggcccga ccgacaccgt gaagctggcg ttcatccgcg agcgcacccg gttcgagaac
     3301 tacgatccca ggagcgtgta ggagcggcca ccgtgatccg ctgcaccttc gcgcacgtcg
     3361 accttggcgc cctcgagtcc aactaccgcg ctatcgaggc gttcctcgcg gcgcggaccc
     3421 cggccggccg ccggcctccg gcaatcgtcg cggtcgtcaa ggcgaacgcc tacgggcacg
     3481 gggccgggcc ggttgccctg gccctcgagc gtgccggggc gaggatggtc gcctgcgccg
     3541 acatcgagga gggcatcgcg ctccgccagg ccggcgtcca cgtgccggtc ctggtgttcg
     3601 gcgcgctgag cgtcagcgat ctcgacggcc tgttcgagta ccggttgacg cccacctgct
     3661 cgacgccggg cgccggcaag gcactgcagg cggccgccgc gc
//
LOCUS       DAOPQX010000370         3697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27123_length_3697_cov_4.745195, whole genome shotgun sequence.
ACCESSION   DAOPQX010000370 DAOPQX010000000
VERSION     DAOPQX010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27123_length_3697_cov_4.745195"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PKK95_15190"
     CDS             <1..950
                     /locus_tag="PKK95_15190"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOC19612.1"
                     /translation="GWGDQRVYPLPAGVLRPGENVVSLNVLSTWDAGGMVGPAEKLAL
                     TFDDGTSVPLAGGWRYQAVPLEIGRPPRAPWEPIAGLTTLYNGMIAPLGTYGLRGVVW
                     YQGETNADAPAGYQELLAGLMSSWRERFGAGLPFLIVQLPGFGSVPAEPVESNWSEVR
                     EAQRRAVAADACAGLVVTIDLGERDDIHPIEKRAVGSRIARAARRVVYGEAVAPSGPV
                     PLSASREAGGVVVTFGDVEGRLVTYSSDRAIGFELCGDGKGSCRYATAQVASNRVVLA
                     PIEGQPLPTRVRFCWGDSPLCNLSDQSGLPVGPFELVIK"
     gene            complement(977..3403)
                     /locus_tag="PKK95_15195"
     CDS             complement(977..3403)
                     /locus_tag="PKK95_15195"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOC19613.1"
                     /translation="MTCARRFVLLVLVPALACVTVLAAQSGNQPPALLSDLVDISRDF
                     RDYSNGFFLADHLGAFDPAAGSGRLAWQRHQLSPRIAFNNMLAVLRPFEGVTFPENEY
                     AVNPELPFSVEFVTPRTVRLRIETGPRTAPESDSLMLAGKVPSDGSWTMSRVEGGYRY
                     ASAGGSVTVFEKPWRLEFRDAKGRLLTKTLHQADMAATLVPGLPFSFLRRSSDYSRSV
                     AASFSLEPGERLFGTGESFTRLDKRGQKVVLWANDANGVETEKMYKPIPFFLSSRGYG
                     MFVHSSTPMTFDMGASFHAANTLLVGDDSLDLFVFLGEPKEVLGEYTALTGRPSMPPL
                     WSFGLWMSRITYFSEQQVREVAAKLRQHRIPDDVIHIDTGWFETDWRCDYQFSKTRFT
                     DPAKMMADLKQDGFHVSLWQLPYFVPKNTLFPEIVSRGLFVKDAGGNLPYEDAVLDFS
                     NPEAVKWYQEKIAALLKMGVGAIKVDFGEAAPLTGVYASGRTGFHEHNLYPLRYNQAV
                     AEITRQVSGESIIWARSAWAGSQRYPLHWGGDSGTLDQSMLSQLRGGLSLGLSGFTFW
                     SHDIGGFGARTPEDLYRRWLPFGLLSSHSRCHGMPPKEPWEYGEAFTDVFRRTAELKY
                     RLMPYVYAQAKDSADRGLPMVRALFVEYPGDPGSWGIEDEYLFGRDVLVAPLFETGAT
                     GRPVYLPPGRWIDYQSGRAYAGGWHTIEAGAIPVVMLVREGAALPHIALAQSTKDMDW
                     SKIELVVYGAAAPSAEALLCLPSDNVLHRVSLPRRGNAYRLASNPLEGRSVLTIRPHS
                     RPARTTETNN"
     gene            complement(3447..>3697)
                     /locus_tag="PKK95_15200"
     CDS             complement(3447..>3697)
                     /locus_tag="PKK95_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOC19614.1"
                     /translation="GGVRVVIYIAMGWVGVVAFRPLLASLGPAGLSLVAGGGIVYTLG
                     VVAYAWRALPYHHAIWHAFVLGGSALHVLAVLWYVIPR"
BASE COUNT          578 a         1232 c         1329 g          558 t
ORIGIN      
        1 tcggctgggg cgaccagcgc gtgtaccccc tcccggccgg cgtgctgcga ccgggcgaga
       61 acgtggtcag cctgaacgtg ctcagcacgt gggatgccgg cggcatggtg ggtcccgccg
      121 agaagcttgc gctgaccttc gacgacggga cgagcgtgcc cctggcgggg ggctggcgct
      181 accaggccgt tccgctcgag atcgggcggc cgccccgcgc gccgtgggag ccgatcgcgg
      241 ggctcaccac gctctacaac ggcatgatcg cgccgctcgg cacctacggg ctgcgcggtg
      301 tcgtctggta ccaaggcgag acgaacgcgg acgcgccggc cggctaccag gaactgcttg
      361 ccggcttgat gtccagctgg cgcgaacgtt tcggcgccgg cctcccgttc ctgatcgtgc
      421 agctgcccgg gttcggctcg gtgccggccg agccggtcga gtcgaactgg tcggaggtgc
      481 gcgaggcgca gcgtcgcgcc gttgcggccg acgcgtgtgc cggccttgtc gtgacgatcg
      541 atctcggcga gcgcgacgac atccatccga tcgagaagcg cgcggtcggc agccgcatcg
      601 cgcgggccgc acgccgggtc gtctacgggg aggccgttgc gccctcgggc cccgtgccgc
      661 tctcggcaag ccgcgaggcc ggcggggtgg tggtgacctt cggggacgtg gaggggcggc
      721 tggtgaccta cagctcggac cgggccatcg gcttcgagtt gtgcggagat gggaagggct
      781 cgtgccggta cgcgaccgcc caggttgcat cgaaccgggt cgtgctggcg ccgatcgagg
      841 ggcagcccct gccgacccgc gtgcgcttct gctggggcga cagcccgctc tgcaaccttt
      901 ctgatcagag cgggctgccg gtcgggccgt tcgagctggt gatcaagtga accgtgtggt
      961 tctcagtcct cagtagtcag ttgttggttt cagttgtccg tgccggtctc gagtgcggcc
     1021 tgatggtcag aaccgaccgg ccttcgaggg ggttggacgc gagccggtag gcgttcccgc
     1081 ggcgcggcag gctgacccgg tgcaggacgt tgtcggacgg caggcagagc agcgcctcgg
     1141 ccgatggcgc cgcagcgccg tagacgacca gttcgatctt cgaccagtcc atgtccttgg
     1201 tcgactgcgc gagggcgatg tgtggcagcg ccgctccctc gcgcaccagc atcaccaccg
     1261 ggatcgcgcc ggcctcgatc gtgtgccagc cgccggcgta cgcgcggccg ctctggtaat
     1321 cgatccagcg gcccggcggc aggtagaccg ggcggccagt cgcgccggtc tcgaagagcg
     1381 gggccaccag cacgtcccgg ccgaagaggt actcgtcctc gatcccccac gagcccggat
     1441 cgcccgggta ctcgacgaac agggcgcgca ccatcggcag cccgcgatcg gcgctgtctt
     1501 tggcctgcgc gtagacgtac ggcatcagcc ggtacttcag ctcggccgtg cgccggaaga
     1561 catcggtgaa cgcctcgccg tactcccagg gctctttcgg cggcatcccg tgacagcggc
     1621 tgtgcgagct gagcaggccg aatggcagcc agcggcggta gaggtcctcg ggcgtgcgcg
     1681 cgccgaagcc gccgatgtcg tggctccaga aggtgaagcc gctgaggccg agcgagagcc
     1741 cgccgcgcag ctgtgacagc atcgactggt cgagggtgcc cgagtcgccg ccccagtgca
     1801 gcgggtagcg ctggcttccc gcccaggcgc tgcgcgccca gatgatcgac tcgcccgaga
     1861 cctgccgggt gatctccgcg accgcctggt tatagcgaag cgggtagagg ttgtgctcgt
     1921 ggaacccggt gcgccccgac gcgtacacgc cggtgagcgg cgcggcctcg ccgaaatcca
     1981 ccttgatcgc gccgacgccc atcttcagca gcgcggcaat cttctcctgg taccacttca
     2041 cggcctcggg gttggagaag tcgagaaccg cgtcctcgta cggcaggttt cccccggcgt
     2101 ccttcacgaa gagcccgcgg ctgacgatct cgggaaagag cgtgttcttc gggacgaagt
     2161 acgggagctg ccagagcgag acgtgaaagc cgtcctgctt caggtcggcc atcatcttcg
     2221 ccgggtcggt gaaccgcgtt ttcgagaact ggtagtcgca tcgccagtcg gtctcgaacc
     2281 acccggtgtc gatgtggatc acgtcgtcgg ggatgcgatg ctggcgcagc ttggccgcca
     2341 cctcgcgcac ctgctgctcc gagaagtacg tgatccggct catccagagg ccgaacgacc
     2401 agagaggcgg catcgagggc cggcccgtca acgcggtgta ctcgccgagg acctccttcg
     2461 gctcgccgag gaagacgaag agatcgagcg agtcgtcgcc gaccaggagc gtgttggcgg
     2521 cgtggaacga cgcccccatg tcgaaggtca tcggcgtcga cgagtggacg aacatcccgt
     2581 agccccggct gctcaggaag aacgggatcg gcttgtacat cttctccgtc tcgacgccgt
     2641 tggcgtcgtt ggcccagagc acgaccttct gcccccgctt gtcgagccgg gtgaacgact
     2701 cgccagtccc gaacaggcgc tcgccgggct cgagcgagaa cgacgcggca acgctgcgcg
     2761 agtagtccga cgagcggcgg aggaacgaga agggcagtcc cggcaccagc gtggccgcca
     2821 tgtctgcctg gtgcagggtc tttgtgagca gccggccctt cgcgtcgcgg aactcgagcc
     2881 gccacggttt ctcgaacacc gtcaccgagc cgcccgcgct ggcgtagcgg tagcctccct
     2941 cgacacggct catcgtccac gagccgtcgc tcggcacctt ccccgccagc atcagcgagt
     3001 cgctctctgg cgcggtcctg gggccagtct cgatccgcag gcgcaccgtt cgaggcgtga
     3061 cgaactccac cgagaagggc agctcggggt ttacggcgta ctcgttctcg ggaaaggtca
     3121 ctccctcgaa cggccgaagc acggcgagca tgttgttgaa ggcgatgcgc ggcgacagct
     3181 ggtggcgctg ccaggcgagc cggcccgatc cggccgcggg atcgaaggcg cccaggtggt
     3241 cggccaggaa gaagccgttg gagtagtcgc ggaagtcgcg gctgatgtcc accaggtcgc
     3301 tgagcagcgc ggggggctgg ttgcccgact gggccgcgag cacggtcacg caggcgagag
     3361 cggggacgag gacgagcagc acgaaccggc gggcgcaagt cacggaacac ctcctcgaag
     3421 tcgtgcgaag cctcgtgagc acgggatcac cgcgggatga cgtaccacag caccgccagg
     3481 acgtgtaggg cgcttccccc gagaacgaat gcgtgccaga tcgcatgatg gtacgggagg
     3541 gcccgccagg cgtaggcgac gacgcccagg gtgtagacga tcccgccgcc ggcgacgagc
     3601 gagaggcccg cggggcccag gctggcgagc agcggacgga aggccacgac cccgacccac
     3661 cccatcgcga tgtagatgac gacgcgcacg cccccga
//
LOCUS       DAOPQX010000371         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27227_length_3686_cov_5.537042, whole genome shotgun sequence.
ACCESSION   DAOPQX010000371 DAOPQX010000000
VERSION     DAOPQX010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27227_length_3686_cov_5.537042"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PKK95_15205"
     CDS             complement(<1..976)
                     /locus_tag="PKK95_15205"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOC19615.1"
                     /translation="MKRLAVVLAAVSALALAGVALRADEGMWMPQQIPQLAAKLKSMG
                     FEGDPRAFADLTGQPMGAVVSLGGCTASFVSPDGLIVTNHHCVTGALQYNSTPERDLL
                     ADGYLAKTREDELQNAPGARVLVTTRVTDVTAAITGNLDPKLTDRQRYDAIDRRLKER
                     VAVCEKGGGVRCSVPSFFEGLKYFEIEQLEIPDVRLVYAPAEGIGVFGGETDNWRWPR
                     HTGDWSFYRAYVGRDGKPAAYSKDNVPYHPKHWLTVAAGGVKPGDLVFVVGYPGRTQR
                     HQTYAEVKATTEWAMPRAIRNAEEQLAIIEKLTKQDKGIALKVAGRIEG"
     gene            1113..1679
                     /locus_tag="PKK95_15210"
     CDS             1113..1679
                     /locus_tag="PKK95_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19616.1"
                     /translation="MRPSLPSVLVAVVAAVVLVSACSKPAEPGASVPSGNAPAAAAAS
                     EAGPVGPTAETSVPMAVAHASDAAPQHGVPPAGDASMPSEPIAQPAGGTSIVEVWANR
                     QALAGKSVTVRGKVVKFHAAIINRNWLHLQDGTGAPADGTHDLTFTTMDVAKVGDIVT
                     ATGTVAVDKDFTAGYKYGVIVESARLAR"
     gene            1751..3181
                     /locus_tag="PKK95_15215"
     CDS             1751..3181
                     /locus_tag="PKK95_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOC19617.1"
                     /translation="MSGFFGVASRTDCVADLYYGTDYHSHLGTRRGGIVTTRQGAFKR
                     TIHDISNAPFRTKFEEELSKHQGSFGVGIISDYEDQPLVIGSHHGVYALATVGRLNNL
                     DALVKRALAVRGTHLSEFKGNEFNPTEVVATLINQQETLAEGIRWAQEQIQGSCSMLL
                     LAPDAIYAARDRLGRTPVIVGRKKGACAVTLESSALPNLGYDSLRDLGPGEIVRLTPE
                     GVEQLAPPGDRMQICAFLWIYYGYPASTYEGVNTEVARNRCGEALARRERVEADLVAG
                     IPDSGIGHAIGYSTQSGVPYRRPFVKYTPTWPRSFMPVEQRVRDLVACMKLIPIRDLI
                     AGKRILFCEDSVVRGTQLRDIITRVFGHGAREVHMRPACPPLLHGCPFLNFSRSKSEM
                     DLAARRAIRELEGGESPHLDDYADPATSRYAAMVEQIRVRLGLTTLRYQTLPDMIDAI
                     GLPASSLCTYCWDGGQQRPRLPLNGG"
     gene            complement(3188..>3686)
                     /locus_tag="PKK95_15220"
     CDS             complement(3188..>3686)
                     /locus_tag="PKK95_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19618.1"
                     /translation="TDVTLWYGGTWGPGRATGGLIYYVQRGAPDTAELFAAYELDAPL
                     APSATLFVDVDEGDGAFLLLAARHSVPLSGAAALDLGVEAGLNLGNAAMGTGEDGEAF
                     TGPYHAEIWAGGSVAIGRGVTLAPRLAVSFPLSSRAGDAIAWAGYDGETRTCVYASLG
                     LTAAF"
BASE COUNT          552 a         1320 c         1276 g          538 t
ORIGIN      
        1 gcccctcgat gcggcccgcc accttgagcg cgatgccctt gtcctgcttg gtcagcttct
       61 cgatgatcgc cagctgctct tccgcgttcc ggatcgcgcg gggcatcgcc cactcggtcg
      121 tcgccttcac ctcggcgtag gtctggtggc gctgcgtccg ccccgggtag ccgaccacga
      181 agacgaggtc gcccggcttg acgccccccg cggccaccgt cagccagtgc ttcgggtggt
      241 acgggacgtt gtccttcgag tacgccgccg gcttgccgtc cctgccgacg taggcgcggt
      301 agaagctcca gtcgccggtg tgacggggcc agcgccagtt gtcggtctcg ccgccgaaca
      361 cgccgatgcc ctcggccggc gcgtagacga gccggacgtc cgggatttcg agctgttcga
      421 tctcgaagta cttcaacccc tcgaagaacg agggcacgct gcagcgcacg ccgccgccct
      481 tctcgcagac ggccacgcgc tccttcaggc ggcggtcgat cgcgtcgtag cgctgccgat
      541 cggtcagctt cgggtcgagg ttgcccgtga tggcggcggt cacgtcggtg acgcgcgtgg
      601 tgaccagcac ccgcgcgccc ggcgcgttct gcagctcgtc ctcgcgcgtc ttcgccaggt
      661 agccgtcggc cagcaggtcg cgctcgggcg tggagttgta ctgcagggca ccggtgacgc
      721 agtggtggtt ggtcacgatc aggccgtcgg gcgagacgaa cgaggcggtg cagccgccga
      781 gcgagacgac ggcgcccatg ggctggccgg tcaggtcggc aaaggccctg ggatcgccct
      841 cgaagcccat gctcttgagc ttcgcggcaa gctgcgggat ctgttgcggc atccacatcc
      901 cctcgtcggc gcgtaaggcg acgccggcca gggcgagagc agagacggcc gcgaggacga
      961 cggcaagacg cttcatcgaa cgctccaggg aagaagaaga cgagcagccc ctagcttaac
     1021 ccgcaaccgc ccgccgatgg gccggggcgg gcgtcgatcg cgggctggat ttcggattgg
     1081 gacgggggtc ggagttgggt tacgctcgtt gaatgcgccc atccctgccc tccgtcctcg
     1141 ttgccgtcgt ggcggccgtc gtcctggtct cggcctgctc gaagcctgcc gagccagggg
     1201 cgtccgtccc ctccggaaac gctcccgccg ccgcggctgc cagcgaggcg gggcccgtgg
     1261 ggccgacagc ggagaccagc gtcccgatgg ctgtcgcgca cgcgtcggat gcggcgcccc
     1321 agcacggcgt gccgcccgcg ggcgacgcgt cgatgccctc cgagccgatc gcgcagcccg
     1381 ccgggggcac cagcatcgtc gaggtgtggg cgaaccgcca ggcgctggcc ggcaagagcg
     1441 tgacggtgcg cggcaaggtg gtgaagtttc acgcggcgat catcaaccgg aactggctcc
     1501 acctccagga cggaaccggc gcaccggccg acggcacgca cgacctgacc ttcaccacga
     1561 tggacgtcgc gaaggtcggc gacatcgtga ccgccaccgg cacggtggcc gtggacaagg
     1621 acttcacggc cggctacaag tacggggtca tcgtcgagag cgcccggttg gccagatagg
     1681 gcgggcgatc gcgcgccctc cgcgcgtccg aagtatactg gccgcttcgt ccccccgggc
     1741 ggaggcatcg atgagcggct tcttcggcgt ggcgtccagg acggactgcg tcgccgatct
     1801 ctactacggc accgattacc actcgcatct cggcacccgg cgcggcggca tcgtcaccac
     1861 ccgccagggc gccttcaagc gaacgatcca cgacatttcg aacgcgccgt tccgaacgaa
     1921 gttcgaggag gagctgtcca agcaccaggg atcgttcggc gtcgggatca tcagcgacta
     1981 cgaggaccag ccgctggtca tcgggtcgca ccacggcgtg tacgcgctgg cgacggtggg
     2041 ccggctgaac aacctggatg cgctcgtgaa gcgggcgctg gccgtgcgcg gcacccacct
     2101 cagcgagttc aagggcaacg agttcaaccc caccgaggtg gtggccaccc tcatcaacca
     2161 gcaggagacg ctcgccgagg ggatccggtg ggcgcaggag cagatccagg gctcgtgctc
     2221 gatgctgctg ctggcgcccg acgccatcta cgccgcgcgc gaccgcctcg gccgcacgcc
     2281 ggtcatcgtc ggccggaaga agggagcctg cgcggtcacg ctcgagagca gcgcgctgcc
     2341 gaacctcggc tacgactcgc tgcgcgacct cgggccgggc gagatcgtcc gcctgacgcc
     2401 ggagggcgtc gagcagctgg cgccgcccgg cgaccggatg cagatctgcg cgttcctctg
     2461 gatctactac gggtacccgg cctccaccta cgaaggggtg aacacggagg tggcccgaaa
     2521 ccgctgcggc gaggcgctcg cccgccgcga gcgcgtcgag gcggacctgg tggccggcat
     2581 ccccgactcg gggatcggcc acgccatcgg ctactccacg cagtcgggcg tgccctaccg
     2641 gcggccgttc gtgaagtaca cgccgacctg gccccgcagc ttcatgccgg tcgagcagcg
     2701 cgtgcgcgac ctggtcgcct gcatgaagct catcccgatc cgcgacctga tcgccggcaa
     2761 gcgcatcctc ttctgcgaag actccgtcgt gcggggaacg cagctgcgcg acatcatcac
     2821 gcgcgttttc gggcacggcg cccgcgaggt gcacatgcgg ccggcctgcc cgccgctgct
     2881 gcacggctgc cccttcctca acttctcgcg gtccaagtcg gagatggacc tggccgcgcg
     2941 ccgcgcgatc cgcgaactcg agggaggcga atccccgcac ctcgacgact acgccgaccc
     3001 ggccacctcg cgctacgccg cgatggtcga gcagattcgc gtccggctcg gcctcaccac
     3061 gctgcgctac cagacgctgc ccgacatgat cgacgccatc ggcctgccgg cctcgagcct
     3121 gtgcacgtac tgctgggatg gggggcaaca gcggccgcgc ctgccgctga acggaggtta
     3181 gtagaactca gaacgccgcg gtgagcccga ggctggcgta cacgcaggtg cgggtttctc
     3241 cgtcgtagcc ggcccacgcg attgcgtctc cggcgcggct tgacagcggg aagctgacgg
     3301 cgagccgcgg cgcgagcgtt accccgcgac cgatcgcgac cgagccgccc gcccagatct
     3361 ctgcgtgata gggaccggtg aaggcctcgc cgtcctcgcc cgttcccatc gcggcgttcc
     3421 cgaggttgag acccgcctcg acgcccaggt cgagtgcggc cgctccagac agcggcaccg
     3481 aatggcgcgc ggcgagcagg aggaacgccc cgtcaccctc gtccacgtcc acgaacaggg
     3541 tggccgacgg ggccagcggc gcgtcgagct cgtacgcggc gaacagctcc gccgtatcgg
     3601 gggcgcctcg ctgcacgtag tagatgagcc cgccggttgc gcgccccggt ccccacgtgc
     3661 cgccgtacca cagcgtcacg tcggtc
//
LOCUS       DAOPQX010000372         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27245_length_3684_cov_4.491871, whole genome shotgun sequence.
ACCESSION   DAOPQX010000372 DAOPQX010000000
VERSION     DAOPQX010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27245_length_3684_cov_4.491871"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="PKK95_15225"
     CDS             <1..876
                     /locus_tag="PKK95_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOC19619.1"
                     /translation="VTTSFGAQEKQSAGMSRPTAREVVAAIQRHVGVPWAAETVDTFK
                     AGSPDTRVTGVAVTMMATMDVLRRAADKGLNLVITHEPTFYTHLDKPEGMPESDPVWA
                     GKRAFIEKHGLVVWRFHDHWHRRDPDGIDAGVTRALGWSGYRKGDRPARFVVPETTVR
                     KLAAEVSQKLDSPVVRVVGDPEMNVSRVGLSLGAAGSTREIGMLERDDVEVLLVGETR
                     EWETVAYAADAATLRKRKALVVIGHIPSEQAGMDECARWLREFVKGVPVELVPAEQPF
                     WTVGVPAATAVRPPR"
     gene            complement(1192..1725)
                     /locus_tag="PKK95_15230"
     CDS             complement(1192..1725)
                     /locus_tag="PKK95_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HOC19620.1"
                     /translation="MPVLPPGAITLPQISWVTGAVRWEEVLGHAAAWNVLVWFATLVA
                     LADGLARVGFVSWIAKSATVPLAGLSPAAAALLLLAPFFASHYLFASVTAHVAALLPV
                     ALAAPVTLPGIDAARFAALLCYSLGLMGVISPYATGPAPVYYASGFLSRGQFWGLGFA
                     FGSVFLLALLALAAITL"
     gene            1738..2058
                     /locus_tag="PKK95_15235"
     CDS             1738..2058
                     /locus_tag="PKK95_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19621.1"
                     /translation="MVIRIWHGWTTPANADIYERMLKEEIFVGIRGRRIRGFKGIQLL
                     RRDAGPEVEFVTVMDFDSIDAVREFAGEDYEAAVVPPKARAVLARFDARSQHYELMER
                     LGAE"
     gene            2088..2561
                     /locus_tag="PKK95_15240"
     CDS             2088..2561
                     /locus_tag="PKK95_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOC19622.1"
                     /translation="MRILSATLVVTALAVLAAACSTERSPSSPSPTPGPSLEQQAYAR
                     VNEYRRSRGLAALAWNDTIAEQARQHSLAMASGRVPFGHDGLDDRLATIRQTIPWSGA
                     AENVALNRTAGGAVDSWLDSSGHLENIVGNYQLTGVGAAAAGSSVYFTQIFIRPR"
     gene            2699..>3684
                     /locus_tag="PKK95_15245"
     CDS             2699..>3684
                     /locus_tag="PKK95_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19623.1"
                     /translation="MAAFTKRAAARCRRSPILAALLSPLLVAAIGQGRVSSPPVAETG
                     HEFVVPAGTAALLQAAGVAVPVEADRALLVLLRELHAGRNDPARIEAVFARAAAAAGE
                     PERVPGLLPPAAWEAVLGRGVAPNDLAAAVLRDRQAAFLYQGLFALDDETLEFFAGNS
                     ALLTAVHRRSAGVFAAYSDGIRVRNGRVEVPGGTAAERAWETALGASPADPARFVASL
                     LESGGGRLAQLFAALGRLDPPHLAFAVGPSARDLPALIEAAAAFDARTQLPFTAWSDV
                     DVPLLLEQVRVTPDGAMAPPRGQSFWDAAFDGRRSLPGEGPPAADVTAAWLVR"
BASE COUNT          584 a         1282 c         1324 g          494 t
ORIGIN      
        1 gtgaccacga gcttcggcgc ccaggagaag caatcggccg ggatgtccag gccgacggct
       61 cgagaagtcg tcgcagcgat tcaaaggcac gtgggagtgc cgtgggccgc ggaaacggtc
      121 gacacgttca aggcggggag ccccgacacg cgcgtcaccg gggtggcggt cacgatgatg
      181 gccacgatgg acgtgctgcg gcgggccgcg gacaagggcc tcaacctcgt gatcacccac
      241 gaaccaacct tctacacgca cctcgacaag ccggagggga tgcctgagag cgacccggtc
      301 tgggccggga agcgggcctt catcgagaaa cacgggctcg tcgtgtggcg cttccacgat
      361 cactggcacc ggcgcgaccc cgacggcatc gacgccgggg tgacgcgcgc gcttgggtgg
      421 agcggctacc ggaaggggga ccggccggcg cggttcgtcg ttcctgagac gacggtgagg
      481 aagctggcgg ccgaggtttc acagaagctg gactctccag tcgtgcgggt cgtgggcgat
      541 cccgagatga acgtcagcag ggtgggactg agccttggtg cggccggatc cacgcgcgag
      601 attggcatgc tggagaggga tgacgtcgag gtgctgctgg tcggggagac gcgcgagtgg
      661 gagacggtcg cctacgcggc cgacgcggcc accttgcgca agaggaaggc cctcgtcgtg
      721 attgggcaca tcccgagcga gcaggcggga atggacgaat gcgcccggtg gctgcgggaa
      781 ttcgtcaagg gcgtgccggt ggaattggtg ccggccgaac agccgttctg gacggtgggc
      841 gtgccagcgg cgaccgcggt gcgtccgcca aggtaacggt tcggttggcg ccgcctgcgt
      901 tggccctcaa tcgatccgtt cgaagaccga taccacgcgg gcgcgcctga cgacccacac
      961 cggcaacagc acgtacccga acaccacgaa ggccagcgcg agaacttcaa ggtcaccgag
     1021 catcgcggcg agttcttcga gtcggacctc gcgcccgttg tgacggccac cgtgcacccc
     1081 tcgtcactgc tccgcgcacc agacgaggag accagacgca gggaaacggc caggttcgtc
     1141 gacgacctga agaaggtggc cgagcgcctg cggtgagatg ccggcgtggg gctacaaagt
     1201 gatcgccgcg agcgcgagca gcgcgagcag gaacaccgag ccgaacgcga agccaagtcc
     1261 ccagaactgg ccccgcgaca gaaaaccgct cgcgtagtac accggcgccg ggccggtggc
     1321 gtacgggctg atgacgccca tcagccccag gctgtagcac agcagcgccg cgaagcgtgc
     1381 ggcatcgatg cccgggaggg ttaccggcgc ggcgagcgcc accggcagca gcgccgcaac
     1441 gtgggcggtg acgctggcga agaggtaatg cgacgcgaag aacggcgcga gcaacagcag
     1501 cgccgccgcg gccggcgaca ggccggcaag cggcacggtc gcgctcttcg cgatccatga
     1561 cacgaagccc accctggcca ggccgtcggc cagcgcgacg agcgtggcga accacacgag
     1621 cacgttccag gctgcggcgt ggccgagcac ctcctcccag cgcaccgcgc cggtgaccca
     1681 ggagatctgc ggtagagtga tggcgccggg cgggagaacc ggcaaaggag cgtgaccatg
     1741 gtcatccgga tctggcacgg ctggaccacc cccgcgaacg ccgatatcta cgagcggatg
     1801 ctgaaggagg agatcttcgt cgggatccgc ggccgccgca tccgcgggtt caaaggcatc
     1861 cagctgctcc ggcgcgacgc cggccccgag gtcgagttcg tcaccgtgat ggacttcgac
     1921 tcgatcgacg ccgtgcgcga gttcgccggg gaggactacg aggccgcggt cgtgccgcca
     1981 aaggcgcgcg ccgtgctcgc gcggttcgac gcccgttcgc agcactacga gctgatggag
     2041 cggctcggcg ccgagtaggg agcggctgcg caacaaggga gggcacggtg aggatcctgt
     2101 ccgcgacgct cgtcgtcacg gcactcgccg tgctcgccgc cgcctgctcg accgagcgca
     2161 gcccttcgtc tccctcgcct acccctggcc cgtcgctcga gcagcaggcc tacgcacgtg
     2221 tcaacgagta ccggcggtcg aggggacttg ccgcactcgc ctggaacgac acgatcgccg
     2281 agcaggcgcg ccagcacagc ctcgccatgg ccagcggccg cgtgcccttc ggacacgacg
     2341 gccttgacga ccgcctcgcc accatcaggc agacgatccc gtggagcggc gccgccgaga
     2401 acgtcgcgct gaaccgcacg gccggagggg cggtggacag ctggctcgac agttccggtc
     2461 acctcgagaa catcgtcggc aactaccagc tcaccggtgt tggcgcggct gcagccggca
     2521 gctcggtgta cttcacgcag atcttcatcc ggcccagata gccctgttcc catctccctc
     2581 cacgtaagag ctttcgcgaa tcgaaagggc gggcgacgtt cgcgcgcgtg cccattacag
     2641 cgaccggccg cccggggccg tggtaccatc ggagtgggtc tacccacagc caagctgcat
     2701 ggccgcgttc accaagcggg cggccgcccg gtgcaggcgg agcccgattc tcgccgcgct
     2761 gctgtcgcca ctgctcgttg cggcgatcgg gcaggggcgc gtttcgtccc cgccggttgc
     2821 cgaaaccggg cacgagttcg tggttcccgc gggcaccgcg gcgcttctgc aagcggccgg
     2881 cgtcgccgtg ccggtggagg cagaccgggc gctgctcgtt ctcctgcgcg agctgcacgc
     2941 cgggcgcaac gaccccgcca ggatagaggc ggtgttcgca cgcgcggccg ccgccgcggg
     3001 cgagcccgag cgcgtgcccg gactgctgcc gcccgccgcc tgggaggcgg tcctcggacg
     3061 gggggtggcg cccaacgatc tggctgccgc ggtgctgcgc gaccggcagg ccgccttcct
     3121 ctaccagggc ttgttcgccc tcgacgacga gacgctcgag ttcttcgccg ggaactccgc
     3181 gctgctgacc gccgtgcacc ggcgctcggc cggagtcttc gccgcctact cggacggcat
     3241 ccgcgtgcgc aacgggcgag tcgaggtgcc gggcggcacg gccgccgaac gcgcgtggga
     3301 gaccgcgctc ggcgcctcgc cggccgatcc cgcccgcttc gtcgccagcc tcctggaatc
     3361 gggcggcggc cggctcgcac agctcttcgc agcgctcggc cggctcgacc cgccgcacct
     3421 cgcgtttgcg gtcggcccgt cggctcgcga cctgcccgct ctcatcgaag ccgccgccgc
     3481 cttcgacgcg cgtacccagc tccctttcac cgcctggtcg gacgtggacg tgccgctcct
     3541 gctcgagcag gtgcgcgtga cgcccgacgg ggcgatggcg ccgccgcgcg ggcagtcttt
     3601 ctgggacgcc gccttcgacg gccggcgctc gctcccgggc gaaggtcccc cggcggccga
     3661 cgtgaccgcg gcatggctgg tgcg
//
LOCUS       DAOPQX010000373         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27371_length_3671_cov_4.572456, whole genome shotgun sequence.
ACCESSION   DAOPQX010000373 DAOPQX010000000
VERSION     DAOPQX010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27371_length_3671_cov_4.572456"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="PKK95_15250"
     CDS             <1..1324
                     /locus_tag="PKK95_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOC19624.1"
                     /translation="LAYSVFSEGGYDIVTMKPRAGRTGDAVMAGSRLELPSVPLPGEK
                     PGRSVEAESATFGARAYRDDMSLESLGQPYLSAGGGGAFGNYLRAGMGFGFGDMLGDQ
                     QLGVAFQGGLRQHDFAARLLYLNRRSRLNWGVSVDYLPTVFGSAKREYDEGAHALRSV
                     AEYRRQSHLQLGGLAIYPFNRSQRVEFSAGFRNVTYGREVETRLIAVPSFRELQRNRE
                     GLPGGDPVQLFETGVALVQDTAVYGPTSPILGGRSRLQVSPSFGSVSYTTVLADYRRY
                     LMPVRPFTLAVRGRYVARLGGGGSDPRLMPLVLSLRDQVRGYNLRTLASRSCEPGQAD
                     CSLFDVLSGSSLIAGNVELRFPIPGFLSGRFNYGPLPVEGFVFADAASVRTRAGTGLG
                     GWRQHLLRSVGAGVRTNAAGVILEMAAARTFDRPGHGWTFAFNLTPGF"
     gene            complement(1326..2453)
                     /locus_tag="PKK95_15255"
     CDS             complement(1326..2453)
                     /locus_tag="PKK95_15255"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOC19625.1"
                     /translation="MSHLRINYRVFTVFLVVGLVMLATAAAVVVGAGQARLRASFGDH
                     LADVASQTAASVDSYVYRRAIETTILASVSDVRREAAQASLAPLDAAAVREFDRVWQQ
                     SRTVPEPLAPITRNAASAFLAETGRNDQTYRELVLADRQGRLAAASGMTTDYFQGDEE
                     WFTRAFGDGVHGRLNVGGVTWDESANTWALEISAPVEDPAGGQLAGVLKAVVDVREMA
                     AAVSAMRLGDTGDAALVTDEGQIVLGSLQPDAETQFFAADLLRERIQGIARQESPPRI
                     SFGARAQDGRARLVGVGVTQLRASYPHLKWLVAVSQAESDLFAPVRFQLWSLLALLAL
                     MAIAVLGLALWFSLRLAAPPPDEELHLVRHAAVGTIDGEGV"
     gene            2604..3365
                     /locus_tag="PKK95_15260"
     CDS             2604..3365
                     /locus_tag="PKK95_15260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOC19626.1"
                     /translation="MLPYHDENRTLRPAYVTLILIAINVGAWIFIQGAGSTLPLARSV
                     CNLGLIPGELTGLLPPGSGFAMGDNMVCLTDPGRQATNVLTSMFLHGSWMHLLGNMWF
                     LWLFGNNVEDSMTRPRFVAFYLLCGLAAALLQVVVDPRSNIPMVGASGAISGVMGAYL
                     VLYPRVRVFTLVPLGFFITSIALPAWVMLLYWGALQLLGGFTSIGAEGGGVAFWAHVG
                     GFVAGLIGVKLFARRDHVRDHRSTRWQPEHVGWDR"
     gene            complement(3441..3534)
                     /locus_tag="PKK95_15265"
     tRNA            complement(3441..3534)
                     /locus_tag="PKK95_15265"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3481..3483),aa:Pro,seq:ggg)
BASE COUNT          506 a         1284 c         1292 g          589 t
ORIGIN      
        1 gctcgcgtac agcgtcttct cggagggcgg ctacgacatc gtgacgatga agccccgggc
       61 cggacggacg ggcgacgccg tgatggcggg ctcgcggctc gagctgccgt cggttccgct
      121 gccgggagag aagccgggac gaagcgtgga ggccgagtcg gcaaccttcg gggcgcgcgc
      181 ctaccgggac gacatgtcgc tcgagagcct cggccaaccc tatctcagcg ccggcggcgg
      241 cggggcgttc ggcaattacc tgcgggccgg catgggcttc ggcttcggcg acatgctcgg
      301 cgaccagcag cttggggtgg ccttccaggg cggcttgcgg cagcacgact tcgccgcgcg
      361 cctgttgtac ctcaaccgcc gatcgcggtt gaactggggc gtgtcggttg actacctgcc
      421 gacggtgttc ggcagcgcga agcgcgagta cgacgagggg gcccacgccc tgcgtagcgt
      481 ggccgagtac cgccggcagt cgcacctgca gctgggcggc ctggcgatct accccttcaa
      541 ccgttcgcag cgggtcgagt tcagtgcagg cttccgcaac gtgacgtacg ggcgcgaggt
      601 cgagacgcgg ctgatcgcgg ttccctcctt ccgggaactg cagcgcaacc gcgaggggct
      661 gccgggcggc gaccccgttc agctgttcga gacaggcgtg gcgttggtgc aggacacggc
      721 ggtgtacggg ccgacgagcc cgattctcgg cggccggagc cggctgcagg tctcgccgtc
      781 cttcggcagc gtcagctaca cgaccgtgct cgccgactac cggcggtacc tgatgccggt
      841 gcgccccttc acgctcgcgg tgcgaggccg gtacgtcgcg cggctggggg gcggcggcag
      901 cgacccgcgc ctgatgccgc tggtcctttc cctgcgcgac caggtgcgcg ggtacaacct
      961 ccgcacgctc gcctctcgca gctgcgagcc ggggcaggcc gactgctcgc tgttcgacgt
     1021 gctgagcggg agcagcctga tcgctggaaa cgtggagctg cgcttcccga tccccgggtt
     1081 cctgtcggga cgcttcaact acgggcccct gccggtcgag gggttcgtgt tcgccgacgc
     1141 cgcgtcggtc agaacgcgcg cgggcacagg gctgggcggc tggcggcagc acctgctgcg
     1201 gagcgtcggg gccggcgtcc ggacgaacgc ggccggcgtc atcctcgaga tggcggcggc
     1261 ccgcaccttc gaccgcccgg gtcacgggtg gacgtttgcc ttcaacctga cgccggggtt
     1321 ctgagctaca ccccctcgcc gtcgatcgtc cccaccgcgg cgtgtctcac caggtgcagc
     1381 tcctcgtcgg gcggcggcgc cgccaggcgc agcgagaacc acagggcgag gccgagcacc
     1441 gcgatggcca tcagcgcgag cagggccagc agtgaccaga gctggaagcg aaccggcgcg
     1501 aacaggtctg actcggcctg cgagaccgcc accagccact tcaggtgcgg gtagctggcg
     1561 cgcagctgcg tgactccaac ccctaccagc cgggcgcgcc cgtcctgcgc ccgggccccg
     1621 aacgagatcc tgggcggcga ttcctgccgc gcgattccct ggatccgttc gcgaagcagg
     1681 tcggccgcga agaactgcgt ctcggcgtcc ggctgcagcg acccgagcac gatctgcccc
     1741 tcgtcggtca cgagggcggc gtcgccggtg tcgccgagcc gcatcgccga gaccgcggcg
     1801 gccatctcgc gcacgtccac cacggccttc aggacacccg ccagctgtcc gcctgccggg
     1861 tcctccacgg gcgcgctgat ttcgagggcc caggtgttcg cgctctcgtc ccaggtgacg
     1921 ccgccgacgt tcagccggcc gtgcacgccg tcgccgaacg cgcgcgtgaa ccactcctcg
     1981 tcgccctgga agtagtcggt cgtcatcccg gacgccgcgg ctaggcgccc ctggcggtcg
     2041 gcgagcacca gctcgcggta ggtctggtcg tttcggccgg tctcggcgag gaacgctgag
     2101 gccgcgttgc gcgtgatcgg ggccagcggc tcggggaccg ttctcgactg ctgccatacc
     2161 cgatcgaact ctctcaccgc ggccgcgtcg agcggggcga ggctggcctg cgccgcctcg
     2221 cggcgcacgt cggacacgga ggcgaggatg gtggtttcga tggcgcgccg gtacacgtag
     2281 ctgtccacgc tagccgccgt ctgcgaggcg acgtcggcga ggtggtcgcc gaacgacgcg
     2341 cgcagcctgg cctgtccggc accgaccacc acggcggcgg cggtggcgag catgacgagc
     2401 ccgacgacga ggaacaccgt gaagacgcgg tagttgatcc gcaggtgcga cacgtcgacc
     2461 tcccggggcg gacgcccctc ggcccacgcg ttgacgcgaa cggtcgggaa ggaatgccga
     2521 gggcagtctg gctccacgcg ccgtgcgtgt caagtcgcgc cgcggctggt agactggagg
     2581 tcaacgcctc gacaggacga cctgtgctcc cataccacga cgagaaccgc acccttcgtc
     2641 ccgcctacgt cacccttatc ctcatcgcga tcaacgtcgg cgcctggatc ttcatccagg
     2701 gcgccgggtc cacgctgccg ctggcccgct cggtctgcaa cctcggcctg attccaggcg
     2761 agctgacggg gctgctgccg ccgggctcgg ggttcgcgat gggcgacaac atggtctgcc
     2821 tcaccgaccc cggccggcag gcgaccaacg tcctcacctc gatgttcctc cacggatcgt
     2881 ggatgcacct gctggggaac atgtggttcc tgtggctgtt cgggaacaac gtcgaggact
     2941 cgatgacgcg gccgcgtttc gtggcgttct acctgctgtg cggcctggcc gcggcgttgc
     3001 tgcaggtggt cgtggatccg cggtcgaaca tcccgatggt gggcgcgtcg ggagccatca
     3061 gcggcgtgat gggcgcgtat ctcgtcctct atccgcgcgt gcgggtgttc acgctggtcc
     3121 cgctcggctt cttcatcacg tcgattgcgc tgccggcctg ggtgatgctg ctgtactggg
     3181 gcgcgctcca gttgctcggc ggcttcacga gcatcggggc cgagggaggc ggcgtcgcgt
     3241 tctgggccca cgtgggcggc ttcgtcgccg ggttgatcgg cgtcaagctc ttcgcccgcc
     3301 gtgatcacgt gcgggatcac cgctcgacgc gctggcagcc cgagcacgtc ggctgggacc
     3361 ggtaagggga tcaggctcgc ggcaagagcc agagacagca aaggccgcgg caccagccgc
     3421 ggcctttcgt ttgtgtcttg tggtcggggc gactggattc gaaccagcga ccccctgaac
     3481 cccattcagg gaaagccccc ttagcagctc ctctcaacgg gtaacacggc cccggtcgac
     3541 tccccgtgtt ccagcctgaa aatagccatt gactcccttc aactgtcgtc atagactctc
     3601 agccaagcgc ggcccctcct gttaccccgg tgttaccccg ccgcgcccgg cactctttca
     3661 gggagccgct c
//
LOCUS       DAOPQX010000374         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27372_length_3671_cov_4.113662, whole genome shotgun sequence.
ACCESSION   DAOPQX010000374 DAOPQX010000000
VERSION     DAOPQX010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27372_length_3671_cov_4.113662"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2912)
                     /locus_tag="PKK95_15270"
     CDS             complement(<1..2912)
                     /locus_tag="PKK95_15270"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOC19627.1"
                     /translation="MEPCAPRQYLVGVDVGSTTVKAVAVEAVTGEIVWSDYQRHETKQ
                     PEKTLEFLERLEADLSLSGGNCRAFITGSGGATIAPLVGAKFVQEVTAVSLAVEKLHP
                     EVHSVIELGGQDAKIIVFKEDPEAGRKQKIPSMNDKCAGGTGAVIDKINAKLKIPADE
                     LGNQGYSGIKLHKVAGKCGVFAETDINGLQKEGTPSDELMASLFEAIVLQNLTVLTRG
                     HTLRPHVLLLGGPNTFIRGMREAWQENIPKMWRERGVEIPPGETPETLIKTPANGVYF
                     AALGAIEFGRTEDEEVGRYRGTRHLEHYITAGRAEQKAAVGAAGLSASPEELARFKEQ
                     YKPAPFTPATFAAGQAVRGFVGVDGGSTSTKAVLMSDEGEVLCKVYQLSKGNPIQDTV
                     DMFEGLREQVEGQGAELEVLGVGTTGYAKDILRDVLHADAAIVETVAHTESALKYYGD
                     PHVIVDVGGQDIKLIVLKDGRVKDFKLNTQCSAGNGYFLQSTAESFGVPVREFADKAF
                     EAKAMPVFGYGCAVFMQSDIVNFQRQGWAPEEILAGLAAVLPKNIFLYVASIPNLAKL
                     GTRFVLQGGTQHNLAAVKAQVDFIRSNFRGADAEPEIIVHQHCGESGAIGAAVEALRL
                     WRGGRRTTFIGLEAVRKISYRSTRNESTRCNFCKNNCLRTFIDIKMAGRAAPAPEQME
                     RVKAEAAAAAPQAKASPARFVSKVPKAADEQRLIIATCEKGTVEDVNEMRGIKANLDA
                     VRKANPNFVEIASKEAFRPRGPARVADPVPARAWTTAGRARIAAMKGRASLRIGMPRV
                     LNMYVYAPFFNAYFESLGVEGTNLVYSDYTTPELYREGSSRGAIDPCFPAKIGIAHVH
                     NLLFVKHAKKPLNYVFFPMVDVLHTPLTHLQGANACPTVTVTPATVKAAFTKESDLFR
                     EHGVEYLNPLVNFGDRELLARQMFDALGVVLGLSEEENARAVEVGFQELEACERDLR"
     gene            complement(2945..>3671)
                     /locus_tag="PKK95_15275"
     CDS             complement(2945..>3671)
                     /locus_tag="PKK95_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19628.1"
                     /translation="RRVSTGKLPAWQLRRRAASALRHRLRMASYGAGRRIFERQYQRL
                     VEGLGGIGHPLSNMQELADLAAPYYHRLARGGEGHLEVAKNIYYTVRRKAHMVLSLKP
                     FGCMPSSQSDGVQSAVMSHFKDMIFLPIETSGEGEINAHSRVQMALGEAKAKARAEFA
                     EALDSTGRTLDEIKAFVQLHPVLRHPFYPVPHREGVTGTAAGFLLHVADVMDGRERLR
                     RVPGAAPSAPAAVTAAARRAAAL"
BASE COUNT          507 a         1326 c         1210 g          628 t
ORIGIN      
        1 cggagatcgc gctcgcaggc ctccagctcc tggaagccca cctccaccgc ccgcgcgttt
       61 tcttcctcgg acaggccgag caccaccccc agcgcatcga acatctgccg ggccagcagc
      121 tcgcggtctc cgaagttcac cagcgggttc aggtactcga cgccgtgctc ccggaagagg
      181 tccgactcct tcgtgaaggc cgccttcacg gttgcgggcg tgaccgtcac cgtcgggcac
      241 gcgttggcgc cctgcaggtg cgtgagcggc gtgtgcagca cgtccaccat cgggaagaag
      301 acgtagttca acggcttctt cgcgtgcttg acgaacagca ggttgtgcac gtgggcgatg
      361 ccgatcttcg ccgggaagca cgggtcgatc gcgccgcggc tcgacccctc gcgatacagc
      421 tccggggtcg tgtagtcgga gtaaaccagg ttggtgccct cgacgcccag gctctcgaag
      481 tacgcgttga agaacggggc gtagacgtac atgttgagca cgcggggcat cccgatgcgc
      541 agcgaggccc gtcccttcat ggccgcgatc cgggcccttc cagcggtcgt ccaggcgcgc
      601 gccggaaccg ggtcggccac ccgggccggg ccgcgcggcc ggaacgcctc cttcgaggcg
      661 atctccacga agttggggtt cgccttgcgc accgcgtcga ggttcgcctt gatcccccgc
      721 atctcgttca cgtcctcgac cgtccccttc tcgcaggtgg cgatgatcag gcgctgctcg
      781 tcggccgcct tcggcacctt ggagacgaac ctggccggcg acgccttcgc ctgcggggca
      841 gccgccgcgg cctcggcctt cacgcgttcc atctgctcgg gggcaggcgc cgcccgcccc
      901 gccatcttga tgtcgatgaa ggtccgcagg cagttgttct tgcagaagtt gcaccgcgtc
      961 gactcgttgc gcgtcgaccg gtagctgatc ttgcggaccg cctccaggcc gatgaacgtc
     1021 gtgcggcggc cgcccctcca caggcgcaac gcctcgacgg ccgccccgat cgcccccgac
     1081 tcgccgcagt gctggtgcac gatgatctcg ggctcagcgt cggcgccgcg gaagttcgac
     1141 cggatgaagt ccacctgcgc cttcacggcc gccaggttgt gctgcgtgcc tccctgcagg
     1201 acgaaccgcg tcccgagctt cgccaggttc gggatgctcg ccacgtaaag gaagatgttc
     1261 ttcggcagaa cggccgccag ccccgccagg atctcctcgg gcgcccaccc ctgccgctgg
     1321 aagttgacga tgtccgactg catgaagacg gcgcagccgt agccgaagac gggcatcgcc
     1381 ttcgcctcga aggccttgtc ggcgaactcg cgcacgggaa cgccgaagct ctcggccgtg
     1441 gactgcagga agtagccgtt gcccgccgag cactgcgtgt tgagcttgaa gtccttgacg
     1501 cggccgtcct tgaggacgat cagcttgatg tcctggcccc ccacgtcgac gatgacgtgc
     1561 gggtcgccgt agtacttcag cgcggactcg gtgtgcgcga ccgtttcgac gatcgccgcg
     1621 tcggcgtgga gcacgtcccg gaggatgtcc ttcgcgtagc cggtggtgcc gacgcccagc
     1681 acttccagct ccgcgccctg cccctcgacc tgctcgcgca gcccctcgaa catgtcgacg
     1741 gtgtcctgga tcgggttgcc cttcgagagc tggtagacct tgcacagcac ctcgccctcg
     1801 tccgacatca ggaccgcctt ggtggaggtc gacccgccgt ccacgcccac gaagccgcgc
     1861 accgcctgcc cggccgcgaa ggttgccggc gtgaaggggg cgggcttgta ctgctccttg
     1921 aagcgagcca gctcctcggg cgacgcgctc aggcccgcgg ccccgaccgc cgccttctgc
     1981 tcggcgcggc cggcggtgat gtagtgctcc aggtggcgcg tcccccggta gcggcccacc
     2041 tcctcgtcct ccgtcctgcc gaactcgatc gcgcccagcg cggcgaagta cacgccgttg
     2101 gccggggtct tgatcagcgt ctccggcgtc tcgccgggcg ggatctcgac cccgcgctcg
     2161 cgccacatct tcgggatgtt ctcctgccac gcctcgcgca tcccccggat gaacgtgttc
     2221 gggccgccga gcagcagcac gtgcggccgc agggtgtggc cgcgcgtcag caccgtcagg
     2281 ttctgcagga cgatcgcctc gaagagggaa gccatcagct cgtctgacgg cgtgccttcc
     2341 ttctggagcc cgttgatgtc ggtctcggcg aagacgccgc acttgcccgc caccttgtgc
     2401 agcttgatcc cgctgtagcc ctggttgccc agctcgtcgg ccgggatctt cagcttcgcg
     2461 ttgatcttgt cgatgaccgc gcccgtgccg ccggcgcact tgtcgttcat cgacgggatc
     2521 ttctgcttgc gcccggcctc cgggtcttcc ttgaacacga tgatcttcgc gtcctggccg
     2581 ccgagctcga tgaccgagtg cacctcgggg tgcagcttct cgacggcgag cgagacggcg
     2641 gtcacctcct gcacgaactt ggcgcccacc agcggggcga tcgtcgcgcc ccccgagccg
     2701 gtgatgaacg cgcggcagtt cccgccgctc agcgacaggt cggcctcgag ccgctcgagg
     2761 aactcgagcg tcttctccgg ctgcttggtc tcgtgcctct ggtagtcgga ccacacgatc
     2821 tcgccggtta ccgcctccac cgccactgcc ttcaccgtcg tcgagccgac gtccacacca
     2881 accaggtact gccgtggcgc acagggttcc atgccggtct ccgtgagctg gtgagtgatg
     2941 cgagctacag cgccgcggcc cgccgcgccg ccgcggtcac cgcggcgggc gcggagggtg
     3001 ccgcccccgg gacgcgccgc agccgctcgc ggccgtccat cacatcggcg acgtgaagca
     3061 ggaagccggc ggccgtgccc gtgacgcctt cccggtgcgg gaccgggtag aacgggtggc
     3121 gcagcacggg atgcaactgc acgaaggcct tgatctcgtc gagggtccgg ccggtcgagt
     3181 cgagcgcctc tgcgaactcg gccctcgcct tggccttcgc ctcgccgagc gccatctgca
     3241 cccggctgtg cgcgttgatc tccccctcgc cggaggtctc gatgggcagg aagatcatgt
     3301 ccttgaagtg gctcatcacg gccgactgca ctccgtccga ctgcgaggag ggcatgcacc
     3361 cgaacggctt gaggctgaga accatgtgcg ccttcctgcg caccgtgtag tagatgttct
     3421 tggcgacctc gaggtgcccc tcgccgccgc gggcgagccg gtggtagtag ggggcggcga
     3481 ggtcggccag ctcctgcatg ttcgagagcg gatggccgat tccgccgagc ccctcgacca
     3541 gcctctggta ctggcgctcg aagatgcggc ggcccgcgcc gtacgaagcc atgcgcaggc
     3601 ggtggcgcaa ggccgaggcc gcccggcggc gcagctgcca cgccggcagc ttcccggtgg
     3661 agacgcgcct c
//
LOCUS       DAOPQX010000375         3661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27469_length_3661_cov_4.358569, whole genome shotgun sequence.
ACCESSION   DAOPQX010000375 DAOPQX010000000
VERSION     DAOPQX010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27469_length_3661_cov_4.358569"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..737
                     /locus_tag="PKK95_15280"
     CDS             180..737
                     /locus_tag="PKK95_15280"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HOC19629.1"
                     /translation="MYLLFLAYPLLITPRLGDRLGPHLTAVLASATFFVIARRQLEAG
                     GYGPVLGLLPLLQAAAMAVLLAALLRLEPPGSRHAARIALAAASLVAFVTIAVPVQFD
                     RQWVTVGWAVEGVALAWVYTWVRRRPLLWAALGLLAVSVLKGFLFDMARLGGLYRVAS
                     FVGLAVCLALAAVVLQRFVFARRAE"
     gene            875..2410
                     /locus_tag="PKK95_15285"
     CDS             875..2410
                     /locus_tag="PKK95_15285"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOC19630.1"
                     /translation="MANRFEDLDQKRLALQQGGGPDRIAKQHAQGKLTARERLSLLFD
                     PDSFVEFGLWVKHRSAELAGKEFPADGVVTGKGEVMGRPTLVFSQDFTVGGGAVGALH
                     AAKIVESMAAALKCGAPLVGFNDGGGARIQEGVEALSGYGRIFYHNTLLSGVVPQISV
                     IAGPCAGGAAYSPALTDFIIMVERTAHMFIAGPEVIRAATGERISEEDLGGASAHASV
                     SGNIHFVAQSDRDAVKLVRTLLSYLPQNNLESPPDAPFSGPVGDDPAVDDLIPDDPRR
                     SYDVKDVVGALVDPGSFLEVQRDWAMNIVVGFARLGGMTVGMVANQPAVLAGALDIDA
                     SDKAARFIRFCNVFNVPIVTLVDVPGFLPGVAQEHGGIIRHGAKMLFAYSASTVPKLT
                     VILRKAYGGAYLAMCSRDLGADSVLAWPTAEIAVMGPEGAARVLYRRELEKAADKQKF
                     LQEKIAEYRRLHANPYRAAEAFHLDDVIRPSWTRRLLIERLGILRAKRDLRPQKKHGN
                     LPL"
     gene            2420..2752
                     /locus_tag="PKK95_15290"
     CDS             2420..2752
                     /locus_tag="PKK95_15290"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOC19631.1"
                     /translation="MEMHPLAVASTAFAIVMAGMAFLVVVLELFRRIGQRSARGAIEA
                     AGAAAAPADGGLDEELVAVLAAAAREALGAPVRLYQVHVHRGGSTEIWSRAGRMDIMV
                     SHRVEPKR"
     gene            2749..3174
                     /locus_tag="PKK95_15295"
     CDS             2749..3174
                     /locus_tag="PKK95_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19632.1"
                     /translation="MKKLRITVNQKVYDVVVQVLEDDEQTVTGAGFMNPLPPPPKSAA
                     VPVAPPAAPAPVPVPPSAAPLKGDPNAILAPIAGVVQKVYVQAGGTVDAKAPVVLLDA
                     MKMDTYIYAPRAGRVAEVSVAAGDAVQVGDRLIRYETEG"
     gene            3175..>3661
                     /locus_tag="PKK95_15300"
     CDS             3175..>3661
                     /locus_tag="PKK95_15300"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOC19633.1"
                     /translation="MLEFLQSTGLAALTWQQAVMIAVSGLLLWLAIAKGFEPLLLVPI
                     GFGALLTNLPLNGLMNPPHGEEIGGLFYYLSYGIRLEIFPPLIFIGLGALTDFGPLLS
                     NPKTLLLGGAAQLGVMGAFLGAWLLGFDVREAASIGIIGGADGPTSIYLTMKLAPHLI
                     GP"
BASE COUNT          486 a         1282 c         1294 g          599 t
ORIGIN      
        1 cccgggtgtt gtccctgatg tcatcgagac catcgaggac ctcgtaagtg acgtacccca
       61 ccgcgagcgc tcccaggatc ttgccaaggt cccctgcgtg ggccctcggc gccgccgtgg
      121 cgtcctcgtc gccgggcggc cttcccgcct gggccgagcc tcttgccgtc gccctccccg
      181 tgtacctgct gttcctcgcg tatccgttgc tcatcacgcc gcggctgggc gaccgattgg
      241 gccctcatct gacggcggtg ctggccagcg ccactttctt cgtcatcgcg cggcgtcaac
      301 tggaggcggg cggatacggg ccggtgctgg gcctgctgcc gctcctgcag gcggccgcca
      361 tggcggtcct gctcgctgcc ctgctgcggc tcgaaccgcc gggctcgcgg cacgcggcgc
      421 ggatcgcgtt ggctgccgcc tcgctcgtcg ccttcgtgac cattgccgtg ccggtgcagt
      481 tcgatcggca gtgggtgacg gtcggctggg cggtggaagg ggtcgcgctc gcatgggtgt
      541 atacctgggt gcgtcggcgt ccactgctct gggcggcgct gggcctgctg gccgtcagcg
      601 tactcaaggg cttcctgttc gacatggcgc gcctcggcgg gctgtaccgg gtggcgtcgt
      661 tcgtcggcct ggcggtctgc ctcgcgctcg ccgcggtcgt gctgcagcgc ttcgtcttcg
      721 cgcgccgcgc cgagtaggcc gtgtttgagc gcccaggccg tttggagtaa tctcaatgac
      781 tcgtgtggcc gcgcccggcg gctcgcgttc gcgctcgcgc cgacgaccac cccggacgct
      841 cccccccctt acgcatccga tcgaggtatc tccaatggcg aaccggttcg aggacctgga
      901 ccagaagagg ctcgccctcc agcagggcgg cggcccggat cgcatcgcga agcagcacgc
      961 ccagggcaag ctgaccgccc gcgagcgtct cagcctcctg ttcgacccgg acagcttcgt
     1021 cgagttcggg ctctgggtga agcaccgctc tgcggagctg gcgggcaagg agttcccggc
     1081 cgacggggtc gtcaccggga agggcgaggt gatggggcgg ccgacgctcg tgttctcgca
     1141 ggatttcacc gtgggcggcg gcgcggtcgg cgcgctgcac gcggcgaaga tcgtcgagag
     1201 catggccgcg gcgctgaagt gcggcgctcc gctggtgggc ttcaacgacg gcggcggcgc
     1261 ccgcatccag gaaggcgtcg aggcgctgtc gggctacggg cgcatcttct accacaacac
     1321 gctgctctcg ggggtggtcc cgcagatctc ggtcattgcc gggccgtgtg ccggcggcgc
     1381 cgcctactcg cccgcgctca ccgacttcat catcatggtc gagcgcaccg cccacatgtt
     1441 catcgcgggg cccgaggtga tccgcgcggc gaccggggag cggatctcgg aggaggatct
     1501 cggcggggcg agcgcccacg cctcggtgtc gggcaacatc catttcgtcg cgcagagcga
     1561 ccgggatgcc gtgaagctcg tgcggacgct gctctcctac ctcccccaga acaacctcga
     1621 gtctcctccc gatgccccgt tcagcggccc cgtgggcgac gaccccgcgg tcgacgacct
     1681 catccccgac gacccgcgcc gcagctacga cgtcaaggac gtcgtcggcg cgctcgtcga
     1741 tccgggcagc ttcctcgagg tgcagcgcga ctgggcgatg aacatcgtgg tcggcttcgc
     1801 gcggctgggc ggcatgacgg tcgggatggt ggccaaccag cccgcggttc tggccggggc
     1861 gctcgacatc gacgcctccg acaaggccgc ccgcttcatc cgcttctgca acgtcttcaa
     1921 cgtgccgatc gtgacgctgg tggacgtgcc cggcttcctc cccggggtgg cacaggagca
     1981 cggcggcatc atccggcacg gcgccaagat gctgttcgcg tactcggcct ccacggtgcc
     2041 gaagctcacc gtcatcctgc gaaaggcgta cggcggggcc tacctcgcga tgtgctcccg
     2101 cgacctcggg gccgactcgg tgctggcctg gccgacggcc gagatcgcgg tgatgggacc
     2161 cgaaggggcg gcgcgcgtcc tctatcgcag ggaactggag aaggcggccg acaagcagaa
     2221 gttcctgcag gagaagatcg ccgagtaccg gcggctgcac gccaaccctt accgggccgc
     2281 cgaggcgttc caccttgacg acgtcatccg cccgtcgtgg acccggcgcc tgctcatcga
     2341 gcggctcggt atcttgcgcg ccaagcgcga cctgcggccg cagaagaagc acggcaacct
     2401 gccgctctga ggcgatcgaa tggagatgca cccgctggcc gtcgcgtcga cagccttcgc
     2461 catcgtcatg gccggaatgg ccttcctcgt ggtggtgctg gaactgttcc ggcgcatcgg
     2521 ccagcggagc gcccgcggcg ccatcgaagc ggcgggcgcg gccgccgccc cggccgacgg
     2581 cggcctcgat gaagagctgg tggcggtgct ggccgccgcc gcccgcgaag cgttgggcgc
     2641 ccccgtccga ctgtaccaag tgcacgttca ccgcggcggc tcgaccgaga tctggtcgcg
     2701 cgccggacgg atggacatca tggtttccca ccgggtggag cccaagcgat gaagaagctg
     2761 cgcatcacgg tcaaccagaa ggtgtacgac gtcgtcgtgc aggtgctcga ggacgacgag
     2821 cagacggtca cgggggcggg gttcatgaac ccgttgccgc cgcccccgaa gagcgcggcc
     2881 gttccggtgg ccccaccggc cgcgccggcg cccgtcccgg ttccgccctc ggcggcgccg
     2941 ctcaagggcg acccgaacgc gatactggcg ccgatcgccg gcgtggtgca gaaggtgtac
     3001 gtccaggcgg gcggcaccgt ggacgccaag gccccggtcg tcctgctcga cgcgatgaag
     3061 atggacacct acatctacgc cccgcgggcc ggcagggtgg ccgaggtgag tgtggcggcg
     3121 ggcgacgcgg tgcaggtggg ggatcggctg atccgttacg agacggaggg ctaggtgctc
     3181 gagttcctcc agtccacggg cctggccgcg ctgacctggc aacaggcggt gatgatcgcc
     3241 gtgtcgggcc tgctcctgtg gctcgcgatc gcgaaaggct tcgagccgct cctgctggtg
     3301 ccgatcggct tcggcgccct gctgaccaac cttccgctca acgggctgat gaacccgccg
     3361 cacggggagg agatcggcgg gctcttctac tacctgtcgt acggcatccg cctcgagatc
     3421 tttccgccgc tgatcttcat cggcctcggg gcgctgaccg acttcggtcc gctgctctcg
     3481 aacccgaaga cgctgctgct cggcggggcc gcgcaactcg gcgtgatggg ggcgttcctc
     3541 ggggcctggc tcctcggctt cgacgtccgc gaggcggcct cgatcggcat catcggggga
     3601 gccgacggcc cgacgtcgat ctacctcacg atgaagctgg cgccccacct gatcgggccg
     3661 a
//
LOCUS       DAOPQX010000376         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27566_length_3650_cov_4.834214, whole genome shotgun sequence.
ACCESSION   DAOPQX010000376 DAOPQX010000000
VERSION     DAOPQX010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27566_length_3650_cov_4.834214"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PKK95_15305"
     CDS             complement(<1..828)
                     /locus_tag="PKK95_15305"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOC19634.1"
                     /translation="MALCFDLNADLGESFGPWRMGLDERVIRFVTSANIACGFHAGDP
                     ATMRRTVCLAEEAGVGIGAHPGFPDLRGFGRRALAASPAEVRDDLVYQIGALTAFTRA
                     KKLQHVKPHGALYNMAVPGGELAAAIAEAVLEVDPSLILVALAGSPWADEAERMGLRV
                     AREAFADRAIQADGSLVPRSRPGAVIHDPHEVVERTLRLVTEGTMVAITGEAVELSAD
                     TLCLHGDTPDAVGLASALRAALEGAGVAGQQDAELRQSKPIGRNSMPAPSSAARRADA
                     "
     gene            939..2120
                     /locus_tag="PKK95_15310"
     CDS             939..2120
                     /locus_tag="PKK95_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOC19635.1"
                     /translation="MADIARIQRELGAAGVDGWLLYDFHNRDAIAYHVLGLDYGKFTS
                     RRWFYWIPASGEPVRLCHKVEARKLDALPGEKRLYLSWRELHASLKALLGGARTVAMQ
                     YSPHANIPYVSVVDGGTIDLVRSLGYEVVSSAGLVQTFQAVLDEAAYESHLEAGKRVQ
                     RIKDEAFALIADSLRAGRTLTQYDVQQFIVRRFGEEGLTCKGEYPIVGTNEQPADPHF
                     EPTPENARPIKAGDTILIDLWAKLDQPKAIFYDITWCGYAGPTPPAKYVEIFHVVRDA
                     RQAALDFIRARYAEGRTLHGWEVDDACRKVVEEAGYGPYFIHRTGHSIGEEVHGNGVN
                     IDNLETKDERELVPGICFSIEPGIYLAGEMAVRSEINVFITPSGQVEVAGEMQRELVL
                     V"
     gene            2185..>3650
                     /locus_tag="PKK95_15315"
     CDS             2185..>3650
                     /locus_tag="PKK95_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOC19636.1"
                     /translation="MTIYLWVILAYLLVMTGYNIYRSRQIKSQDDFMVAGRSLSLTKM
                     VFTLVCTWIGSGTFIAGAEYASYAGWSALWQPAGAFLGIAIIYFVAGKIRTFGQYTVG
                     DILEVRYGRFARLFGAIALIIAFTTIVAYQFRAGGYILNVISDGRISLELGQTITAIF
                     VIGFVAIGGMIAVAHTDLPNGIIIVSACLIAVPFVITTAGGWAHAHEVLEPSRFQVFA
                     SDFGKFPPLKAFAYFLSTLLLLLGVQSMYQKFYAARSPREARQAVAIWIVGTIVVETI
                     VIAIAVFASVYNTEHALGWDGRALVLNAARYMVPGWAGVLLLGAACAVVLSTGMNYLL
                     SSSSNVMRDIYQSMINPNADPDKMVALQKVFIVVMGFCAFLMIFIPTYMKSQISILQY
                     AYFAYTMYGVAVTPALFAALTWKRATWQGGVASIVGGAVATVFFEFVLPALAPGVMQG
                     AGGAAGAVFGADPWGIPSIYPSAIISIGALVLVSLMTPA"
BASE COUNT          525 a         1246 c         1288 g          591 t
ORIGIN      
        1 cgcgtcggcc ctgcgcgccg cgctggaggg cgccggcatc gagttgcgcc cgatgggctt
       61 cgactgacga agctccgcat cctgctggcc ggcgacgccg gcgccctcca gcgcggcgcg
      121 cagggccgac gcgaggccga ccgcgtcggg cgtgtcgccg tgcaggcaga gggtgtcggc
      181 cgacagctcg acggcctcgc cggtgatggc gaccatcgtt ccctcggtca cgagccgcag
      241 cgtgcgttcc accacctcgt gcgggtcgtg gatcacggcg ccgggacgcg agcgggggac
      301 gagcgatccg tcggcctgga tggcccggtc ggcgaacgcc tcgcgggcca cgcgcaggcc
      361 catccgctcg gcctcgtcgg cccagggcga gccggccagc gcgacgagga tcagcgaggg
      421 gtccacctcg aggaccgcct cggcgatcgc cgccgccagc tcgccgccgg gcaccgccat
      481 gttgtagagg gcgccgtggg gcttgacgtg ctgcagcttc ttcgcgcgcg tgaacgcggt
      541 gagcgcgccg atctggtaga cgaggtcgtc gcgcacctcg gcgggagacg ccgcgagggc
      601 gcggcggccg aagccgcgga gatcgggaaa gcccgggtgg gccccgatgc ccacgccggc
      661 ctcctcggcc aggcagaccg tccgccgcat cgtggccggg tcgccggcgt ggaacccgca
      721 cgcgatgttc gccgaggtga cgaagcggat gacccgctcg tcgagtccca tgcgccacgg
      781 gccgaagctc tcgccgagat cggcattcaa gtcgaagcag agcgccatgc ccacccccgt
      841 tggaccggta tgttagcccg gctgcccgcc tcgccgcagc ccccgcctat gctattctgc
      901 cccggccggt ggctgccgcc ggcggacgag gaggcgacgt ggctgacatc gcacgtatcc
      961 agcgcgaact gggcgccgcc ggggtggacg gctggctgct ctacgacttc cacaaccgcg
     1021 atgccattgc gtaccacgtg ctcggcctgg actacggcaa gttcacgagc cgccgctggt
     1081 tctactggat cccggcaagc ggcgagcccg tgcgcctttg tcacaaggtc gaagcccgga
     1141 agctcgacgc gcttcccggc gagaagcggc tctacctctc ctggcgcgag ctgcacgcca
     1201 gcctgaaggc gctgctcggc ggcgcgcgga cggtggcgat gcagtactcg ccgcacgcga
     1261 acatccccta cgtgtcggtg gtggacggcg gcacgatcga tctcgtgcgg tcgctcggct
     1321 acgaggtggt gtcgtcggcc ggcctggtgc agaccttcca ggcggtgctc gacgaggcgg
     1381 cctacgagtc gcacctcgag gcggggaagc gggttcagcg gatcaaggac gaggcgttcg
     1441 ccctgatcgc cgactcgctg cgcgccggcc ggacgctgac gcagtacgac gtgcagcagt
     1501 tcatcgtgcg gcgcttcggg gaggaggggc tcacctgcaa gggcgagtac ccgatcgtcg
     1561 gcaccaacga gcagccggcc gacccgcact tcgaaccgac gcccgagaac gcccggccga
     1621 tcaaggcggg cgacaccatc ctgatcgacc tctgggcgaa gctcgaccag ccgaaggcga
     1681 ttttctacga catcacctgg tgcggctacg cgggcccgac gccgccggcg aagtacgtcg
     1741 agatcttcca cgtggtccgc gacgcccggc aggcggccct cgacttcatc cgcgcccgct
     1801 acgccgaggg gcgtaccctt cacggctggg aggtggacga cgcgtgccgg aaggtggtgg
     1861 aggaggcggg gtacgggccg tacttcatcc accggaccgg gcactcgatc ggcgaggagg
     1921 tgcacggcaa cggcgtgaac atcgacaacc tggagaccaa ggacgagcgc gagctggtgc
     1981 ccggcatctg cttctcgatc gagcccggga tctacctcgc cggggagatg gcggtgcgct
     2041 cggagatcaa cgtcttcatc acgccgtccg gccaggtcga ggttgccggg gagatgcaaa
     2101 gggagctcgt gctggtgtag gggcaggggc aggcgcggga cgcctgccct actgtgtccg
     2161 ctgtctgctg tcgtggagga gcgtgtgacc atctacctgt gggtcatcct ggcgtacctg
     2221 ctggtgatga ccggctacaa catctaccgg tcgcgccaga tcaagtcgca ggacgacttc
     2281 atggtggcgg ggcgcagcct gtcgctcacc aagatggtct tcacgctggt gtgcacgtgg
     2341 atcggctccg gcaccttcat cgccggcgcc gagtacgcct cgtacgccgg ctggagcgcc
     2401 ctctggcagc ccgccggcgc cttcctcggc atcgccatca tctacttcgt ggccgggaag
     2461 atccgcacct tcggacagta caccgtgggc gacatcctcg aggtgcgcta cggccggttc
     2521 gcgcgcctct tcggcgccat cgcgctcatc atcgccttca cgacgatcgt cgcctaccag
     2581 ttccgcgccg gcggctacat cctcaacgtg atcagcgacg ggcggatctc gctcgagctg
     2641 ggccagacga tcacggcgat cttcgtcatc gggttcgtgg ccatcggcgg gatgatcgcg
     2701 gtcgcccaca ccgacctgcc gaacggcatc atcatcgtct cggcctgcct gattgccgtc
     2761 ccgttcgtga tcaccacggc gggcggctgg gcgcacgcgc acgaggtgct cgagccgtcg
     2821 cgcttccagg tgttcgcctc ggacttcggg aagttcccgc cgctgaaggc gttcgcctac
     2881 ttcctctcga ccctgctgct gctgctgggc gtgcagtcga tgtaccagaa gttctacgcg
     2941 gccaggtcgc cgcgcgaggc gcggcaggcg gtcgcgatct ggatcgtcgg caccatcgtg
     3001 gtggagacga tcgtgatcgc catcgcggtc ttcgcctcgg tgtacaacac cgagcacgcc
     3061 ctcggctggg acgggcgcgc gctggtgctg aatgccgcgc gctacatggt gcccgggtgg
     3121 gccggcgtgc tgctgctcgg ggccgcctgc gcggtcgtgc tctcgaccgg catgaactac
     3181 ctgctgtcgt cgagctcgaa cgtgatgcgc gacatctacc agtcgatgat caacccgaac
     3241 gccgatcccg acaagatggt cgccctgcag aaggtcttca tcgtcgtcat ggggttctgc
     3301 gcgttcctga tgatcttcat ccccacctac atgaagtcgc agatctccat cctgcagtac
     3361 gcctacttcg cgtatacgat gtacggggtg gcggtgacgc ccgcgctgtt cgcggcgctc
     3421 acctggaagc gggcgacctg gcagggcggg gtggcctcga tcgtcggtgg cgcggtggcg
     3481 acggtgttct tcgagttcgt gctgccagcc ctcgcgccgg gcgtgatgca gggggccggc
     3541 ggggccgcgg gcgcggtgtt cggcgccgac ccgtggggga tcccgagcat ctacccgtcg
     3601 gcgatcatct cgatcggcgc gctggtgctg gtgagcctga tgacgccggc
//
LOCUS       DAOPQX010000377         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27650_length_3641_cov_5.534579, whole genome shotgun sequence.
ACCESSION   DAOPQX010000377 DAOPQX010000000
VERSION     DAOPQX010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27650_length_3641_cov_5.534579"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKK95_15320"
     CDS             <1..295
                     /locus_tag="PKK95_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19637.1"
                     /translation="FESFRPGRTRTPFDLSGRLTGLRTDGGVTWVAVGVNGVVRAVTR
                     TWESEPDRWLATPPLDAWKRDHRNEVEIFVVGGDERSPVLRRAKLSRTSTAGG"
     gene            392..613
                     /locus_tag="PKK95_15325"
     CDS             392..613
                     /locus_tag="PKK95_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19638.1"
                     /translation="MRLLTPLLLACAFLLAWQPPVEAYLDPGSGSMLIQLLLGGVAGL
                     AVILKLFWRRILDILHIGGGHDRERPGDA"
     gene            606..2009
                     /locus_tag="PKK95_15330"
     CDS             606..2009
                     /locus_tag="PKK95_15330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOC19639.1"
                     /translation="MPEAGVEPGSFRDRSARVFIQDDEVFRGLNERALEQWNTLSATA
                     FFRRFVADGGLVGTERLDPAGRTLPAFAAEWAAVLRHDRIPFVSYPYEWSFGMLRDAA
                     LLQLDLLLAALDEGMTLKDASAYNVQWRGAKPVFVDIGSFERRPPGEPWIGYRQFCQL
                     FLYPLLLTAYRDIPFHPWLRGSLEGIDPESCLNALGLGGRLRRGVLSHVYLQARAQAA
                     YGGTRRDVKADLRAAGFDSALIKANATRLRKLIAGLTWKRGRSTWSEYTKCGHYEAAD
                     AEAKRRFVGRAAASGSRRLVWDLGCNTGTFSRIAAEHAGYVIALDADHLAVERLYQAL
                     KAENDRRILPLVGNLADPSPDLGWRNMERKRLASRGRPDLVLALALIHHVVIGANVPI
                     DEFVDWLADLGGDIVIEFVTREDPMVGVLLRNKEDQYADYQIDVFERCLRARCEILER
                     EPLGSGTRVMYWARPRG"
     gene            2017..3639
                     /locus_tag="PKK95_15335"
     CDS             2017..3639
                     /locus_tag="PKK95_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOC19640.1"
                     /translation="MLLKRLNTEDLDLIVKTLHEFTAREAPLDQRLKWDETDRCPAEV
                     VREMLGPEVGLHLVFLPEEFGGMGGGAYDVYRLSCEFAKIDLGLATGMLAVALGTDPI
                     RVGCTEEQKAKWIPRIAEEGLIVAYGVTEPEAGSNVQSLKTEATPIADADGRVTHYRL
                     NGVKQFISNGSIADLYTILAKAPGGPTFFVVERQTPGLTPGRHEIKHGIRLSDTAQVL
                     LQDVVIPAGNIVGEREGDGLKQANEVFGYTRLMVAAFGLGAGLSALEKAIAYSKQRKQ
                     FGTFLAQKQGFTHKLLVPDSVRLAAARAYIEYVADLLDGGTADRQVEGSVAKLFATEA
                     GNLAADHAVQALGGYGYCVDFEVEKIRRDARILTIYEGTSEIQQSIIGIFRMREAVKS
                     KGQFYLRMAEGVEPLAEVGGPAVARAARFLADAAQTAFRAKLVRQQHVAFEYATAMTE
                     VETAVALAKAAAAQDGHLMKAQSRLWASQVALSVPSRILTALTASDGLGAEELTRLRK
                     LGDLEGALALQSGGLADMNLVAEGITGMRVGE"
BASE COUNT          545 a         1279 c         1280 g          537 t
ORIGIN      
        1 cttcgaatcg ttccggcccg gccggacgag aactcccttc gacctgtcgg gccggctcac
       61 cggccttcgc actgacggcg gcgtcacctg ggtggcggtg ggggtgaacg gggtcgtgcg
      121 cgcggttact cgcacgtggg agagcgaacc ggaccgctgg ctcgccacgc cgcccctcga
      181 cgcctggaag cgcgaccacc gcaacgaggt ggagatcttc gtcgtcggcg gtgacgagcg
      241 gtcgccggtg ctgcgccggg ccaagctgtc tcgaaccagc accgcgggag gctgacgccc
      301 ctttctcttg cccgcccgtg ctacactcac cggcggtctc ctcggggacc gccgcgttct
      361 ttcggaaccg ccgagctctt ccagacagcc tatgcgccta ctgacaccgc tcctgctcgc
      421 gtgtgcgttc ctgctggcct ggcagccgcc cgtcgaggcg tacctcgacc cgggcagcgg
      481 cagcatgctc atccagctgc tgctgggcgg cgtcgcggga ctcgccgtca tcctgaagct
      541 cttctggcgc cgcatcctcg acatcctgca catcggcggc gggcacgacc gggagcgtcc
      601 gggcgatgcc tgaggcgggt gtcgaaccgg gttccttccg cgaccggtcc gcgagagtct
      661 tcatccagga cgacgaggtt ttccgggggc tgaacgagcg cgcgctcgag cagtggaaca
      721 ctctctcggc caccgccttc ttccgccgct tcgtcgccga cggcgggctg gtcggcaccg
      781 agcgcctcga tccggccggg cgcacgctgc cggcgttcgc ggccgagtgg gccgcagtcc
      841 tccgccacga ccggattccc ttcgtctcgt acccgtacga gtggtcgttc gggatgctgc
      901 gcgacgcggc gctgctgcag ctcgacctgc tgctcgcggc gctcgacgag gggatgacgc
      961 tcaaggacgc ctctgcctac aacgtgcagt ggcgcggggc gaaaccggtc ttcgtggaca
     1021 tcggctcctt cgagcggcgg ccgccgggcg aaccgtggat cggctaccgc cagttctgcc
     1081 agctgttcct gtatccgctg ctgctcaccg cctaccgcga catcccgttc cacccctggc
     1141 tgcgcggcag cctggaaggg atcgacccgg agagctgcct caacgcgctc ggcctcggcg
     1201 ggcggctgcg gcgaggcgtg ctgtcgcacg tgtacctgca ggcgcgggca caggccgcct
     1261 acggcggcac gcggcgcgac gtcaaggccg acctccgcgc cgcaggcttc gacagcgcgc
     1321 tgatcaaggc gaacgccacg cgcctgcgga agctgattgc cggcctcacc tggaagcggg
     1381 gccggtccac ctggtcggag tacaccaagt gcggccacta cgaggccgcc gacgccgagg
     1441 cgaagcggcg gttcgtcggc cgcgccgccg cgtcgggcag ccggcgcctg gtgtgggacc
     1501 tgggctgcaa caccggcacc ttctcgcgca tcgccgccga gcacgccggc tacgtcatcg
     1561 ccctggacgc cgaccatctc gcggtcgagc gtctctacca ggcgctcaag gccgagaacg
     1621 acaggcggat cctccccctg gtcgggaacc tggccgaccc gtcgccggac ctcgggtggc
     1681 gcaacatgga gcggaagcgc ctggcctcgc gcggccggcc cgacctggtg ctggccctgg
     1741 cgctcatcca ccacgtcgtc atcggcgcca acgtcccgat cgacgagttc gtggactggc
     1801 tggcggacct cggcggcgac atcgtgatcg agttcgtgac ccgcgaagac cccatggtgg
     1861 gcgtgctgct gcgcaacaag gaagaccagt acgccgatta tcagatagac gtgttcgagc
     1921 ggtgcctgcg ggcccggtgc gagatcctcg agcgcgagcc gctcggctcg ggaacgaggg
     1981 tcatgtactg ggcgcgcccg cgagggtgag gactgaatgc tgctgaagcg cctgaacacc
     2041 gaggacctcg acctgatcgt caagacgctg cacgagttca ccgcgcgcga ggcgccgctc
     2101 gaccagcggc tgaagtggga cgagacggat cgctgcccgg ccgaggtggt tcgcgagatg
     2161 ctcggccccg aggtcggcct gcacctggtg ttcctcccgg aggagttcgg cggcatgggc
     2221 ggcggcgcct acgacgtgta ccgcttgtcg tgcgagttcg cgaagatcga cctcggcctg
     2281 gcgaccggca tgctcgcggt ggcgctcggc accgacccga tccgcgtcgg ctgcaccgag
     2341 gaacagaagg cgaagtggat cccgcggatc gccgaggagg gcctgatcgt cgcctacggc
     2401 gtgacggagc ccgaggccgg gtcgaacgtg cagagcctga agaccgaggc gacgcccatc
     2461 gccgacgccg acggccgagt gacgcactac cgcctgaacg gcgtgaagca gttcatctcg
     2521 aacgggtcga tcgccgacct ctacacgatc ctcgccaagg ctcccggagg cccgaccttc
     2581 ttcgtggtcg agcgccagac gccgggcctg acgccgggcc gccacgagat caaacacggg
     2641 atccgcctct cggacaccgc gcaggtgttg ctccaggacg tcgtcatccc ggccggaaac
     2701 atcgtcggcg agcgcgaggg cgacggcctg aagcaggcca acgaggtctt cgggtacacg
     2761 cgcctcatgg tggccgcctt cggcctgggc gccggcctct cggcgctcga gaaggccatc
     2821 gcgtactcga agcagcggaa gcagttcggc accttcctgg cgcagaagca gggcttcacc
     2881 cacaagctgc tcgtgcccga cagcgtgcgg ctggcggcgg cgcgggccta catcgagtac
     2941 gtcgccgacc tgctcgacgg cgggaccgcc gaccgccagg tggaagggtc ggtcgcgaag
     3001 ctgttcgcca ccgaggccgg caacctcgcc gccgaccacg ccgtccaggc gctcggcggc
     3061 tacggctact gcgtggattt cgaggtggag aagatccgcc gcgacgcgcg catcctcacc
     3121 atctacgaag gcacctcgga gatccagcag agcatcatcg ggatcttccg gatgcgagag
     3181 gcggtcaaga gcaaggggca gttctacctg cggatggccg agggggtcga gccgcttgcc
     3241 gaagtgggcg ggccggccgt ggcgcgcgcc gcgcgcttcc tggccgacgc ggcgcagacg
     3301 gccttccgcg ccaagctggt gcggcagcag cacgtcgcct tcgagtacgc caccgcgatg
     3361 accgaggtcg agaccgccgt ggcgctcgcg aaagccgccg ccgcgcagga tggccacctg
     3421 atgaaggcgc agtcgcgact ctgggcgtcg caggtggccc tctcggtccc ctcgcggatc
     3481 ctgacggcgc tcacggcctc ggacggccta ggcgcagagg aactcacccg cctgcgaaag
     3541 ctcggagacc tcgagggggc ccttgccctc cagtccggcg gcctggccga catgaacctc
     3601 gtggcggagg ggatcacggg gatgagggtg ggcgagtagt t
//
LOCUS       DAOPQX010000378         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27746_length_3632_cov_4.464076, whole genome shotgun sequence.
ACCESSION   DAOPQX010000378 DAOPQX010000000
VERSION     DAOPQX010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27746_length_3632_cov_4.464076"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PKK95_15340"
     CDS             complement(<1..997)
                     /locus_tag="PKK95_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19641.1"
                     /translation="MPTRSAASLLWQTLRAHWAASADEGAAGGLVVLTRQEWYERLHS
                     ALPGSARRLAPLEREVLLAAAAREAAAGGEAPPFALRPGLIAEMLAFYDTLLANRRTV
                     DRLESFLVADLEPRVDIDRGAERLLRQTRFLVAAYRGFQARMAEAGGMDEDGLRQALL
                     RSDVAAPWNEMVIATADRAADPAAGLHPADLDLLARLPGLRSVAFVATSRVLAAGLGE
                     RLHDAFPDLEDDNWKPLSGESTVATAPQPGSGPPCFRHRDREEELRATARRFGQLVGS
                     ADPAAPPRLALVFGRPLPYVYLARTAFADAGLGVEVADELPLAAEPFAALLELIVQ"
     gene            complement(1123..2517)
                     /locus_tag="PKK95_15345"
     CDS             complement(1123..2517)
                     /locus_tag="PKK95_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19642.1"
                     /translation="MPRFPALPVAALLAALAGAALPRAATAQDPAHLPLFRVILKDGT
                     ALASVGECSRAGDRVIFSMPLGPAGQDRLQLVNLPAGAVDWESTSRYTEAVRYARYVA
                     TRAEMDYAVLTGEVAAALNEIAQVRDPARRLAIAEQAQRLVNGWPAQHFGYRASEVRE
                     MASLLDETISDLRAAAGVQQFDFNLVAMVQPPSMPLLPPPSRAQSIEQALVAARASDV
                     PVERMALLQAVIAILDEPDSSLPASWRRLAKASAEATLEAEMNVERRYESLADSVLRA
                     SERAAARADVRGVERAIQTLRRRDERLGGKRPDRVQGLMAALEERLDAARRLRVMRDQ
                     WQLRARAFEEYSKAIEEGVRQAERLLPRLDDIRSLAGPPVDRLPALAARFDAIYRRLA
                     AVTAPAEMAGAHGTLLSAVDLGLQAVRTRTRAAVSGDVKAAWDASAAASGAVMLLAQA
                     RQSIAVLSKPPELK"
     gene            2802..3020
                     /locus_tag="PKK95_15350"
     CDS             2802..3020
                     /locus_tag="PKK95_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19643.1"
                     /translation="MIPVRRFQHVIAGCVYDIEASRVREDRWRAQIARIPGVPTAMMP
                     FYGRTPDEAATHLCEWLARAHRLRPASS"
     gene            complement(3036..>3632)
                     /locus_tag="PKK95_15355"
     CDS             complement(3036..>3632)
                     /locus_tag="PKK95_15355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOC19644.1"
                     /translation="YLAERSGVLGVLTVADVVRPESREAVDRLRSQGIEVVMLTGDQQ
                     AVAEAVASELGIRKVFAEVLPERKSQVIEELRGSGRRVAMVGDGVNDAPALVTADLGI
                     AIGAGTDVAVEAGDVVLVRSDPRDVARIVALSRASYRKMVQNLWWAAGYNVVAIPLAA
                     GVLAPWGIVLSPAVGAVLMSASTVIVAANAQLLRREPL"
BASE COUNT          503 a         1334 c         1345 g          450 t
ORIGIN      
        1 cctggacgat cagctcgagc agcgcggcaa acggctcggc ggcgagcggc agctcgtcgg
       61 cgacctcgac accgagaccc gcgtcggcga acgcggtacg cgcgaggtac acgtaaggca
      121 acggccggcc gaacaccagc gcgaggcgtg gaggggcagc cgggtcggca ctgccaacga
      181 gctggccgaa gcgccgggcc gtcgcgcgca gttcctcctc gcgatcgcga tggcggaagc
      241 agggtggacc gctgcccggc tggggcgcgg tcgccacggt actctccccc gagaggggtt
      301 tccagttgtc gtcttcgagg tcggggaagg catcgtgcag acgctcgcca agcccggcgg
      361 caagcacgcg cgacgtggcg acgaaggcga ccgaacggag gcccgggagg cgagccagca
      421 ggtcgaggtc cgccgggtga aggccggcgg cgggatcggc cgcgcgatcc gcggttgcga
      481 tcaccatctc gttccacggc gccgcgacgt ccgagcgcag cagcgcctgc cgcaggccgt
      541 cctcgtccat gccgccggcc tcggccatgc gggcctggaa tccccggtac gccgccacga
      601 ggaaccgcgt ctggcgcagc agccgctcgg cgccgcggtc gatgtccacg cgcggctcca
      661 ggtcggcgac gaggaacgac tcgagccggt ccaccgtgcg ccggttggcg agcagcgtgt
      721 cgtagaacgc cagcatctcg gcgatcaggc cgggccgcag ggcgaagggc ggcgcctcgc
      781 cgcccgcggc ggcctcgcgg gcggccgccg ccaggaggac ctcccgctcg agcggcgcca
      841 ggcgccgggc cgatcccggc agcgccgagt gcagcctctc gtaccattcc tgcctggtca
      901 gcacgaccag gccgcccgcg gcgccctcgt ctgccgaggc cgcccaatgc gcccgcaggg
      961 tttgccatag cagagatgcc gccgaacgcg tgggcacgag cacggcgaga cgcccggccc
     1021 gccggccggg gcgcgccggc gcgcacagcg acgcgagcgc cgcgtgcaga gcgcgtggac
     1081 cgtgggctcg aacgagccgg atgcgtcggg gcgtgggctg gttcacttca gctcgggcgg
     1141 cttggagagg acggcgatcg actgtctcgc ctgcgccagc agcatgaccg cgcccgaggc
     1201 ggcggccgac gcgtcccacg cggccttgac gtcgccgctc accgccgcgc gcgtgcgggt
     1261 gcggacggcc tggaggccga ggtcgacggc ggacaggagc gtgccgtgcg cgccagccat
     1321 ctcggcgggg gcggtcacgg ccgcgagccg ccggtagatc gcgtcgaaac gggccgcgag
     1381 cgccggaagg cgatcgaccg gcgggccggc gagggaccgg atgtcatcga gccgggggag
     1441 caggcgctcg gcctgcctca cgccctcctc gatcgccttc gagtattcct cgaacgcgcg
     1501 ggcgcgcagc tgccactggt cgcgcatcac ccgcaggcgc cgcgcggcgt cgaggcgctc
     1561 ctcgagcgcg gccatcagcc cctggacgcg atcggggcgc ttgccgccga gccgctcgtc
     1621 gcgccgccgg agcgtctgga tggcgcgctc cacgccgcgc acgtcggcgc gcgcggccgc
     1681 gcgctcgctt gcgcgcagca ccgagtcggc gagcgactcg tagcgccgct cgacgttcat
     1741 ctcggcctcg agcgtcgcct cggccgaggc cttcgccagg cgccgccacg aggcggggag
     1801 actggagtcg ggctcgtcga ggatggcgat caccgcctgc agcagcgcca tgcgctcgac
     1861 cggcacgtcc gaggcgcgcg ccgcaacgag cgcctgttcg atcgactggg cccgtgacgg
     1921 cggcggcagc agcggcatcg acggcggctg caccatggcg acgaggttga agtcgaactg
     1981 ttggacgccg gcggcggcgc gcaggtcgga gatcgtctcg tcgagcagcg acgccatctc
     2041 ccgcacctcg gacgcgcggt acccgaagtg ctgggcgggc cagccgttga ccagccgctg
     2101 cgcctgctcg gcgatcgcca gccgcctggc ggggtcgcgc acctgggcga tctcgttgag
     2161 cgcggccgcc acctcccccg tgagcacggc gtagtccatc tcggcgcggg tcgcgacgta
     2221 ccgcgcgtag cggaccgcct cggtgtagcg gctcgtgctc tcccagtcca ccgcgccggc
     2281 cggcaggttc accagctgga ggcggtcctg gcccgcgggc cccagcggca tggagaagat
     2341 gacgcggtcg cccgcccggc tgcactcccc caccgacgcg agggcggtcc cgtccttcag
     2401 gatcacgcgg aagagcggga ggtgcgcggg gtcctgcgcc gtcgccgccc ttggcagggc
     2461 tgcgcccgcg agcgccgcga gcagcgcggc cacggggagc gccgggaatc tgggcatgcg
     2521 cgtcaagcgt aagggccggg gcgcccaagc gtcaaggccg cacgaagggc tgcgcacgaa
     2581 cggtgcgcgg agggcgaaag gggcggtgtg gaaaagctgt ggagcggcgg tgggtttgtt
     2641 gtgtgaggcg cgacagcgtg cagcgcgggg tcacggcccc gcttggcctg gcgccccgtc
     2701 gaggcttgtg cacctcgtct tcgtcggtcc tttcgcttga aacgccggtg cagccccgta
     2761 gaatggcttc gtcctttcac acgtcctcgg cactgaccac catgatcccc gttcgccgtt
     2821 tccagcacgt catcgccggc tgtgtctacg acatcgaggc ctcgcgcgtc agggaggatc
     2881 gctggcgagc gcagatcgcc cgcattcccg gcgtgccgac ggccatgatg cccttctacg
     2941 gccgcacccc ggacgaagcg gcaacgcacc tgtgcgagtg gctcgcccga gcgcaccggc
     3001 tccgccccgc gagctcgtag gggcgtcaat tccgctcaga gcggctcgcg gcgcagcagc
     3061 tgcgcgttcg ccgcgacgat caccgtgctc gccgacatga gcaccgcgcc caccgctggc
     3121 gagagcacga tgccccacgg ggccagcacg ccggccgcca gcgggatcgc caccacgttg
     3181 tagccagcag cccaccagag gttctgcacc atcttccggt agctggcgcg gctgagcgcg
     3241 acgatgcgcg cgacgtcgcg cggatcgctg cggacgagca cgacgtcgcc ggcctcgacg
     3301 gccacgtcgg tgccggcgcc gatcgcgatg ccgaggtcgg cggtgaccag agcgggggcg
     3361 tcgttcacgc cgtcgccgac catcgcgacg cgccggcccg aaccccgcag ctcttcgatg
     3421 acctgcgact tcctctcggg cagcacctcg gcgaagacct tccgtatccc gagttccgac
     3481 gcgacggcct cggcaaccgc ctgctggtcg ccggtgagca tcacgacctc gatgccctgg
     3541 ctcctcaagc ggtccaccgc ctcgcgcgac tcggggcgca cgacgtcggc caccgtgagc
     3601 acgccaagaa cgcccgagcg ctcggccagg ta
//
LOCUS       DAOPQX010000379         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_27823_length_3623_cov_4.736267, whole genome shotgun sequence.
ACCESSION   DAOPQX010000379 DAOPQX010000000
VERSION     DAOPQX010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27823_length_3623_cov_4.736267"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKK95_15360"
     CDS             <1..303
                     /locus_tag="PKK95_15360"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOC19645.1"
                     /translation="SIGMMRFVTLTPELAEEYGLSVDRGVVVLQMTRSSSAYRAGIRP
                     GDVIVSFNGQRVDDDGQLRKLLLDAQVGTTVTIQVLRDGRTVSVKVPVVPRVQGRG"
     gene            511..1806
                     /locus_tag="PKK95_15365"
     CDS             511..1806
                     /locus_tag="PKK95_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19646.1"
                     /translation="MTLFRAAVTVSAVAILSAGVAGAGVPAQEARAFEAVVADLKSPD
                     ARTRLEAVRQLAAAGHPEAALPLATVLADPDDRVAREAMYAELSLYLGTRVRGERRVA
                     FVVEMPRSNPASRAFEDSWGSLPVTPVPGEVIEGLSRRLTGEDLMLRLEATAALGVLG
                     QVEGALTPAHEKAADALAQRLGDPKPPIRAAAARAAGRMFRRCAAPCAAPGIERLGDS
                     LVRALNDPEAPVLMAVIEGLGEMRYARATEALASVYDHFQKKPPALAALDALARIGSP
                     SSVPLFKAAAARKEPAARRAALEGLARAGERDALSAAVTQAQAVRADTEVVLGAAFAL
                     QRAGLGQHLSPLLNAAGGDDTALQVQDYLVELGPEVAPQVAAALPAARGEARARLIEV
                     LGVIGGPGQVRAVEAARGEGGARVAAAAERALKRLAASR"
     gene            1803..2459
                     /locus_tag="PKK95_15370"
     CDS             1803..2459
                     /locus_tag="PKK95_15370"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOC19647.1"
                     /translation="MILPRAFYSRPTLTVAPDLLGKVLVHRSPEGLTAGAIVEAEAYI
                     GEDDPACHASAGLTPRTAPLFGPPGHAYVYLNYGIHFLFNVVTEEDGHPAAVLIRALE
                     PLEGLPLMRKRRARDRRSGVVPDHELCRGPGALARAMGIDLRHNRADLCEAGQGGRRR
                     PSGLSGLYLEDRGIRFEPIAWSARIGVSAGADRPWRCYVAGCSSVSGRREQRVTAEES
                     "
     gene            complement(2478..>3623)
                     /locus_tag="PKK95_15375"
     CDS             complement(2478..>3623)
                     /locus_tag="PKK95_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOC19648.1"
                     /translation="AGEQGGKVVFSGTLDELMRDTRSLTARYLRGELSIPVPRLRRRG
                     NGHRLEILGASEHNLKRIDVSIPLNTLTCITGVSGSGKSTLVHDIIHPAVKRAKGGYE
                     GRVGAHRGVIGTDGVTDVVLIDQAPIGRTPRSNPVTYLKAFDPIRELFASTRDARSRG
                     LTASHFSFNVPGGRCEACEGEGQIRVEMQFLADVFVPCEQCDGKRFKPQVLEVKYRGK
                     DVHQVLEMTVREALAFFSGSPKVLRRLHVLDEIGLGYLRLGQPATTLSGGEAQRIKIA
                     SHLASKTADRLLYIMDEPTTGLHFDDIAKLLAAFRKLLQAGHSLLVIEHNLDVLKTAD
                     HIIDLGPEGGEEGGFVVVAGTPEQVAACPRSYTGQHLAPILAGAREA"
BASE COUNT          469 a         1295 c         1335 g          524 t
ORIGIN      
        1 tcgatcggga tgatgcgctt cgtcaccctg acccccgagc tggccgagga gtacgggctc
       61 tcggtcgatc gcggcgtggt cgtcctgcag atgacgcgca gttcgtcggc gtaccgcgcg
      121 ggcatcaggc ccggcgacgt gatcgtgagc ttcaacgggc agcgggtgga tgacgatggg
      181 cagctgcgga agctgctgct cgacgcgcag gtggggacca cggtcaccat ccaggtgctg
      241 cgcgacggcc gcacggtcag cgtcaaggtc cccgtcgtcc cgagggtgca ggggaggggc
      301 tgaccccgct cctcgcgtgt gttgagcaag gacatgggcg ttcgcgggtc ctgccgcggg
      361 gctccccccg ctcggcttcg cgcgaaaagc gcttgtcacg cgtgtcgcgc tccaggcgag
      421 cgggggcctt ccgccccgcg tcacccgctc acggtcttgc ccggcgcaag ggcggggatc
      481 aagggccgcg aatggaccat gagcatattc atgacgttgt ttcgcgccgc ggtaacggtg
      541 agtgcggtgg cgatcctctc ggccggagtg gcgggggccg gcgtcccggc gcaagaggcg
      601 cgtgcgttcg aggctgtcgt cgccgacctg aagtcgcccg acgcgcggac ccgcctcgag
      661 gcggtcaggc agctcgcggc cgccggccat cccgaggcag ccctgccgct cgcgaccgtg
      721 ctcgccgatc ctgacgaccg ggtcgcacgg gaggcgatgt acgccgagct gagcctctac
      781 ctcggcacac gcgtgcgcgg cgagcgccgg gtggcgttcg tggtcgagat gccccgatcg
      841 aatcccgcct cgcgagcctt cgaagactcg tggggctcgc tgccggtcac gccggtgccg
      901 ggcgaggtga tcgaagggtt gtcgcgccgc ctgaccggtg aagacctgat gctgcgcctc
      961 gaggccactg ccgcgctcgg cgtgctgggg caggtggagg gcgcgctgac acccgcacac
     1021 gagaaggcgg ccgacgcgct ggcccagcgg ctgggcgacc ccaagccgcc gatccgggcg
     1081 gccgcggcgc gggccgccgg gcggatgttc cgccggtgtg cggcgccgtg cgcggccccg
     1141 ggcatcgagc ggctcggcga ctcgctggtg cgcgcgctca acgaccccga ggcgccggtc
     1201 ctgatggcgg tgatcgaggg actcggcgag atgcggtacg cgcgcgcgac cgaggcgctc
     1261 gcgtccgtgt acgaccactt ccagaagaag ccgcccgccc tcgccgcgct cgacgcgctg
     1321 gcgcgaatcg gctcgccgtc ctcggtgccc ctcttcaagg cggcagccgc gcgcaaggag
     1381 ccagccgccc ggcgggctgc gctcgagggc ctcgcacgtg ccggcgagcg cgatgccttg
     1441 agcgcggcgg tcacccaggc gcaggccgtc cgggccgaca ccgaggtggt gctcggggca
     1501 gcgttcgccc tgcagcgggc gggtctcggc cagcacctgt cgcccctgct caacgcggcg
     1561 ggcggcgacg acacggcctt gcaggttcag gactacctcg tcgagctggg gccggaggtc
     1621 gcaccccagg tggcggcggc gctgccagcg gcaaggggcg aggccagggc gcggctgatc
     1681 gaggtgctgg gcgtcatcgg cggccccggg caggtccgcg ccgtcgaggc cgcccgcggt
     1741 gagggcggcg cgcgcgtcgc cgcggccgcc gagcgggcgc tcaagcgcct cgccgcgtcc
     1801 cgatgatcct cccccgggct ttctactcgc gccccaccct gacggtggcg ccagaccttc
     1861 tcgggaaggt tctcgtccac cggtctcccg agggcctgac ggccggggcc atcgtcgagg
     1921 ccgaagccta catcggtgag gacgatcccg cgtgccacgc ctcggcggga ctcaccccac
     1981 ggacggcgcc gctcttcggc ccgcccgggc acgcctacgt ttacctgaac tacggcatcc
     2041 acttcctctt caacgtggtc accgaggagg acggccaccc ggccgccgtg ctgatccggg
     2101 cgctcgagcc gctcgaggga ctgccgctga tgcggaagcg gcgagcccgc gaccggcgct
     2161 cgggcgtggt gcccgaccac gagttgtgca ggggacccgg cgcgctcgcc cgcgcgatgg
     2221 gaatcgacct ccggcacaac cgggccgacc tgtgcgaagc cggccagggc ggccggcgca
     2281 ggccttcagg cctgtccggt ctgtatctcg aggatcgcgg gatccggttc gagccgatcg
     2341 cctggtcggc gcgcatcggc gtctcggctg gcgccgatcg tccctggcgc tgctacgtcg
     2401 cgggttgctc ctcggtctcc ggacggaggg aacaacgggt gacggccgag gaatcctgat
     2461 gccacggcca ccaggtccta cgcttcgcgg gcgccggcga ggatcggagc gaggtgctgc
     2521 ccggtgtagg agcgcgggca ggcggccacc tgctcgggcg tgccggcgac gaccacgaag
     2581 ccgccctcct cgcccccctc ggggccgagg tcgatgatgt ggtccgccgt cttgagcacg
     2641 tcgaggttgt gctcgatgac cagcagcgag tggccggcct gcagcagctt gcggaaggcg
     2701 gcgagcagct tggcgatgtc gtcgaaatgg aggccggtgg tcggctcgtc catgatgtag
     2761 agcagccggt cggccgtctt cgaggcgagg tgcgaggcaa tcttgatccg ctgcgcctcg
     2821 ccccccgaga gcgtcgtcgc cggctgcccg aggcgcaggt agcccaggcc gatctcgtcg
     2881 agcacgtgca ggcgccgcag caccttgggc gagcccgaaa agaacgccag cgcctcgcgg
     2941 acggtcatct cgagcacctg gtgcacgtcc ttcccgcggt acttcacctc cagcacctgc
     3001 ggcttgaagc gcttcccgtc gcactgctcg cagggcacga agacgtcggc caggaactgc
     3061 atctcgaccc ggatctgccc ctccccctcg cacgcctcgc agcgcccccc gggcacgttg
     3121 aacgagaagt ggctcgcggt caggcccctc gaccgcgcgt ccctggtcga ggcgaacagc
     3181 tcgcggatcg ggtcgaacgc cttcaggtag gtcaccgggt tcgaccgcgg cgtgcgcccg
     3241 atcggcgcct ggtcgatcag gacgacgtcg gtgacgccgt cggtgccgat gacgccgcga
     3301 tgggcgccga cgcggccctc gtagccgccc ttcgcccgct tcaccgccgg gtggatgatg
     3361 tcgtgcacca gcgtcgactt ccccgatccg ctcacgcccg tgatgcaggt gagtgtgttc
     3421 agcgggatcg agacgtcgat ccgcttcagg ttgtgctcgc tggcgccgag gatctcgagc
     3481 cggtgcccgt tgccgcggcg gcgcaggcgc ggcaccggga tgctcagctc gccgcgcagg
     3541 tagcgcgcgg tcaacgaccg cgtgtcgcgc atcagctcgt cgagcgtccc gctgaagacc
     3601 accttcccgc cctgctcccc ggc
//
LOCUS       DAOPQX010000380         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_28239_length_3582_cov_4.207258, whole genome shotgun sequence.
ACCESSION   DAOPQX010000380 DAOPQX010000000
VERSION     DAOPQX010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28239_length_3582_cov_4.207258"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PKK95_15380"
     CDS             <1..1194
                     /locus_tag="PKK95_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOC19649.1"
                     /translation="RRARGRNLFFSTDIACGIRDADIIFVSVNTPTKTFGEGCGRAAD
                     LQFWEKTARQILANSTGNKVVVEKSTLPVRTAQAMDRILNSNTRGLRFEVVSNPEFLA
                     EGSAIRDLENPDRVLIGSHETPEGLRARRAVADLYATWVPRDRIIESNIWSAELSKLT
                     ANAFLAQRISSINSISALCEVTEADVDEVAYAIGSDSRIGSRFLKASVGFGGSCFHKD
                     ILNLVYIAESYGLHEVAHYWESVVRMNDWQESRFVHRMLANMFNTVAGKRIALFGFAF
                     KANTGDTRESPAIAVTRQLLADRAHVVITDPKALDNARLDLAGVDGQLEFEPDPYKAA
                     EGSHAIAVMTEWDVYKKLDYKRILASMERPAFVFDGRNILDHRLLFELGFNVFPIGKS
                     PLRQP"
     gene            1340..3247
                     /locus_tag="PKK95_15385"
     CDS             1340..3247
                     /locus_tag="PKK95_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC19650.1"
                     /translation="MQRTLPRLLETSAERFPDNVLLLEKRAERYEGTTYREARERVHR
                     FGAGLMDLGLQKGDRVALLSEGRNDWVVAELGILCCGAVNVPISVKIDEASALKFRLL
                     HSGCRMAVVSRGQVEKIRAVRNDLPDLQLTIVLDPLEPLEPDEVWAGDVAARGERLLA
                     GSRAAFDARWQGIAEPDPANICYTSGTTADPKGIVLTHRNYTANIEQGVATIHPQPDW
                     VLLNVLPWDHAFGHTCGIYMMMMSGAALASVQAGRSAMEAARNIPVNIRETRPTIFLS
                     VPAIAKNFRKNIEKAVRDKGPRAAALFERALRTAYAYNAEGWNRGAGARKLRKPLVLL
                     YDRLLFRKIRENFGGRLQYFVGGGALLDIELQRFFYAIGIPMLQGYGLTEAAPVISAN
                     TLEHHKLGSSGRVLPNMEVRICDPEGRDLPTGGRGEIVVRGENVMAGYWRNDKATAET
                     IRDGWLYTGDLGYFDQDGFLYVLGRVKSLLIADDGEKYSPELIEEAITGGSPYIEQVM
                     LYNDQSPYTTALLVPARDALRGWLKARGLSAAAQEGQEAALRLIEGEIAKYRDGGERA
                     GMFPGRWLPSAVAVLGEGFTEQNHMLNSTLKMVRGKIVDYYRSRIDYLYTPEGRSILN
                     EQNRRIVSRLD"
     gene            complement(3297..>3582)
                     /locus_tag="PKK95_15390"
     CDS             complement(3297..>3582)
                     /locus_tag="PKK95_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19651.1"
                     /translation="LGRATTAARGVGRTMKEAEDVKRAAETVEALQRQVAELEAELAA
                     QAAAIETTMTAGPLEQLEIAPKKSGIGVQVVALTWAPYWEDERGGRSAAY"
BASE COUNT          586 a         1260 c         1211 g          525 t
ORIGIN      
        1 cgccgcgccc gcggccgcaa ccttttcttc tcgaccgaca tcgcctgcgg catccgcgac
       61 gccgacatca tcttcgtctc ggtgaacacc cccaccaaga ccttcggcga ggggtgcggc
      121 cgggccgccg acctgcagtt ctgggagaag acggcgcgcc aaatcctggc gaactccacc
      181 ggcaacaagg tcgtcgtcga gaagtcgacg ctgccggtca ggacggccca ggccatggac
      241 cggatcctca acagcaacac ccgcggcctg cgcttcgagg tggtgtcgaa ccccgagttc
      301 ctggccgagg gctcggcgat ccgcgacctc gagaaccccg accgcgtgct catcggctcg
      361 cacgagaccc ccgaggggct gcgggcgcgc cgcgccgtgg ccgacctcta cgccacgtgg
      421 gtgccgcgcg accggatcat cgagtcgaac atctggagcg ccgagctgtc gaagctgacc
      481 gcgaacgcct tcctcgcgca gcgcatctcg tcgatcaact cgatctccgc gctgtgcgag
      541 gtcaccgagg ccgacgtgga cgaggtggcg tacgcgatcg gcagcgactc gcgcatcggc
      601 tcccgcttcc tgaaggcgag cgtcggcttc ggcggctcct gcttccacaa ggacatcctc
      661 aacctggtct acatcgccga gtcgtacggc ctgcacgagg tggcgcacta ctgggagtcg
      721 gtcgtgcgga tgaacgactg gcaggagagc cgcttcgttc accggatgct cgccaacatg
      781 ttcaacacgg tggcgggcaa gcggatcgcc ctcttcgggt tcgccttcaa ggcgaacacg
      841 ggcgataccc gcgagtcgcc ggcgatcgcc gtcacgcgcc agctcctggc cgaccgcgcg
      901 cacgtcgtga tcaccgaccc gaaggcgctc gacaacgccc ggctcgacct ggccggcgtg
      961 gacgggcagc tcgagttcga gcccgaccct tacaaggcgg ccgagggctc gcacgcgatt
     1021 gccgtgatga ccgagtggga cgtgtacaaa aagctcgact acaagcggat cctggcgtcg
     1081 atggagcggc ccgccttcgt gttcgacggc cgcaacatcc tcgaccaccg gctcctgttc
     1141 gagctgggct tcaacgtgtt cccgatcgga aagagcccgc tgagacagcc gtagaagaag
     1201 gctcagggtt caaggctcac ggctcagggc tcaaggctca cgggcctgcc ccgccatagc
     1261 cgcgagcgta gcgggcgttt ggcgaaggcg ggcacgcccc ccggtttccc ctgcatctcc
     1321 gcccccgggt ggatcaagaa tgcagcgcac cctgccgcga ttgctggaga cgagcgccga
     1381 gcgctttcct gacaacgtcc tgctgctcga gaagcgcgcc gagcgctacg aggggacgac
     1441 ctaccgcgag gcgcgcgagc gcgtgcaccg cttcggcgcc gggctgatgg acctggggct
     1501 ccagaagggc gaccgtgtcg ccctcctctc cgaggggcga aacgactggg tcgtcgccga
     1561 gctgggcatc ctttgctgcg gcgcggtgaa cgtcccgatc tcggtcaaga tcgacgaggc
     1621 gtcggccctg aagttccgcc tcctgcactc gggctgccgg atggcggtcg tctcgcgcgg
     1681 ccaggtcgag aagatccgcg ctgtcaggaa cgacctgccc gacctgcagc tgacgatcgt
     1741 tctcgacccg ctcgagccgc tcgagcccga cgaggtctgg gcgggcgacg tcgcggcccg
     1801 gggcgaacgc ctgctggccg gcagccgcgc cgcgttcgac gcgcgctggc aggggattgc
     1861 cgagccggac cccgccaaca tctgctacac ctcggggacg acggccgacc cgaaggggat
     1921 cgtgctgacc caccggaact acacggccaa catcgagcag ggggttgcca ccatccaccc
     1981 acagcccgac tgggtgctgt tgaacgtcct gccctgggac cacgccttcg gccacacctg
     2041 cggcatctac atgatgatga tgagcggcgc cgcgctcgcc tcggtgcagg cgggccggag
     2101 cgcgatggag gcggcgagga acatcccggt caacatccgc gagactcgcc cgacgatctt
     2161 cctgagcgtc cccgccatcg cgaagaactt ccgcaagaac atcgagaagg cggtccgcga
     2221 caaggggccg cgcgccgcgg cgctgttcga gcgggcgctg aggacggcgt acgcctacaa
     2281 cgccgaaggg tggaaccggg gcgccggcgc caggaagctg cgcaagccgc tcgtcctgct
     2341 ctacgaccgg ctgctgttcc ggaagatccg cgagaacttc ggcggccggc tgcagtactt
     2401 cgtcggcggg ggcgcgctgc tcgacatcga gctgcagcgg ttcttctacg cgatcggcat
     2461 ccccatgctg cagggctacg gcctcaccga ggcggcgccg gtgatctcgg ccaacacgct
     2521 cgagcaccac aagctcgggt cgtcggggcg cgtgctgccc aacatggagg tccgcatctg
     2581 cgacccggag gggcgcgacc tgccaaccgg cgggcgcggc gagattgtcg tccgcggcga
     2641 gaacgtgatg gccggctact ggcggaacga caaggcgacc gccgaaacga tccgcgacgg
     2701 ctggctctac accggcgacc tcgggtactt cgaccaggac ggcttcctct acgtcctcgg
     2761 gcgggtgaag agcctcctca tcgccgacga cggggagaag tacagcccgg agctgatcga
     2821 ggaggccatc accggcgggt cgccgtacat cgagcaggtg atgctctaca acgatcagtc
     2881 gccctacacg accgcgctgc tcgtgcccgc gcgcgacgcg ctgcgcggct ggctgaaggc
     2941 gcgaggactg tcggcggcgg cccaggaggg ccaggaggcg gcgctccggc tgatcgaggg
     3001 ggagatcgcg aagtaccgcg acggcggcga gcgggcgggg atgttccccg ggcgctggct
     3061 gccctcggcg gtggcggtgc tcggcgaggg gttcaccgaa cagaaccaca tgctcaactc
     3121 gacgctgaag atggtgcgcg ggaagatcgt cgactactac cgcagccgca tcgactacct
     3181 ctacaccccg gaaggacggt cgatcctgaa cgagcagaac cggcggatcg tgagccgcct
     3241 ggattagcag cagaccagta gcacaggcgt cccgcctgtg ggcactgcct ccagtgtcag
     3301 tacgcggcgc tccggcctcc ccgctcatcc tcccagtaag gcgcccaggt gagggcgacc
     3361 acctgcacgc cgatccctga cttcttcggc gctatctcca gctgctccag cggtcccgcc
     3421 gtcatcgtcg tctcgatggc ggcggcctgc gcggccagct cggcctcgag ctcggcgacc
     3481 tggcgctgca gcgcctccac cgtctcggcc gcccgcttga cgtcctcggc ctccttcatc
     3541 gtgcggccca ctccgcgggc cgcggtcgtg gcgcgtccga gg
//
LOCUS       DAOPQX010000381         3579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_28263_length_3579_cov_6.194098, whole genome shotgun sequence.
ACCESSION   DAOPQX010000381 DAOPQX010000000
VERSION     DAOPQX010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28263_length_3579_cov_6.194098"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1604
                     /locus_tag="PKK95_15395"
     CDS             <1..1604
                     /locus_tag="PKK95_15395"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOC19652.1"
                     /translation="LKCETVSLYQGGQHWLYKYKRYDDVRLVFAPEYAIAAFGGDPDN
                     FQFPRWCLDMSLLRAYENGKPASTPGHLSVNWAGAKQGEAAFVAGHPGTTQRLLTVAQ
                     LKAQRDVFLPFWLIRFSELRGRLAQYSKTSPEAARTATDYLDTVENSLKVRRMQLATL
                     LDDRLMERREEEERKLREAVQADQALKALAGEAWDEIARAEATYRKILGPYTWLEGGA
                     GFNSDLFGYARMIVRAAEESAKPNAERLREYTDAALEQVRQALAAEVPIYPELEKVRL
                     SFSLERMREYLGPDHPVVKRVLGPATPDERARELIAGSTLASPKARLALFEGGQAAVN
                     ASKDPMIALAKAVDAEARALRKVYEEQVQVPEQRAQQAIADARFKVYGTSIYPDATFT
                     LRLSYGSVQGWNESGRPVDPFTQLSGLFERATGAPPFALPKAWLEAKPRLDMTTRANF
                     TTTNDIVGGNSGSPVINAAGEIVGLIFDGNIHSISGGFWYDEEMNRAVAVHPAFIRTA
                     LENVYKVPGIAGELGIQATVKNTAR"
     gene            1684..2064
                     /locus_tag="PKK95_15400"
     CDS             1684..2064
                     /locus_tag="PKK95_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19653.1"
                     /translation="MAVWLRYLFAFLLFAHGFIYIRVGGALPGTVKGWNGRPWLLSKA
                     ITGGALITLLRAVHVAAGIAIVACAIAIALNHPSWRTFAILGCAVGLVAFAIFWDGQT
                     SLLFDEGAVGAVISLALLLTAILA"
     gene            2305..2841
                     /locus_tag="PKK95_15405"
     CDS             2305..2841
                     /locus_tag="PKK95_15405"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC19654.1"
                     /translation="MATGTSMSPDKAALGPRQRFLDTLEREHETTMKVLRAYPADRLD
                     LRPHPRCNTARDLMWTLVMEQKLAQKALTIGFDWSKAPGSPPPPPQQLDEIIDTLERE
                     HRRVVALVKDLPDQKLHETVTFPTAPKTMGQWPIDQFLWMLLHDQIHHRGQLSIYLRM
                     ADGKLPSIYGPTADEPWL"
     gene            complement(2900..>3579)
                     /locus_tag="PKK95_15410"
     CDS             complement(2900..>3579)
                     /locus_tag="PKK95_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19655.1"
                     /translation="VAAAASKQVTRGPGGDFQPNWSPDGRTLAFFSSRSGSPGIWTVN
                     LESGDLASLSPRGGIEINPFYSPDGRLIAYQSDRLGRLEVWVMNADGSEPRPLTTVGA
                     MGHFLRWTRDGRYVIFRCPGGGKPLTLRVPVEGGEAEPLGEIAGGAHMSMSPDESLVL
                     DVVNHKVLHVSRVGQGAPEAVFEFDDPEVRIDYPVWSPDGTLASFDRFVPRGGDIWML
                     EEGLAGR"
BASE COUNT          568 a         1287 c         1181 g          543 t
ORIGIN      
        1 cgctcaagtg cgagaccgtc tcgctgtacc agggcggcca gcactggctc tacaagtaca
       61 agcgctacga cgacgtgcgg ctggtgttcg cgccggagta cgcgattgcg gcctttggcg
      121 gcgaccccga caacttccag ttcccgcgct ggtgcctcga catgtcgctg ctgcgcgcct
      181 acgagaacgg caagccggcc tcgacgcccg gccacctgtc ggtcaactgg gcgggcgcga
      241 agcagggcga ggccgcgttc gtggccggcc accccggcac cacgcagcgc ctgctcaccg
      301 tggcgcagct gaaggcgcag cgcgacgtct tcctcccctt ctggctgatc cggttctccg
      361 agcttcgcgg ccgcctcgcg cagtactcga agacatcgcc cgaggccgcg cgcaccgcca
      421 ccgactacct cgacaccgtc gagaacagcc tcaaggttcg ccgcatgcag ctcgcaaccc
      481 tgctggacga tcggctgatg gagcggcggg aggaggagga gcgcaagctg cgcgaagcgg
      541 tgcaggccga ccaggccctg aaggcgcttg ccggcgaggc ctgggacgag atcgcgcgcg
      601 ccgaggcaac ctaccggaag atcctcgggc cgtacacctg gctcgaggga ggcgccggtt
      661 tcaacagcga cctcttcggc tacgcgcgca tgatcgtgcg ggcggccgag gagagcgcca
      721 agccgaacgc cgagcggctg cgcgagtaca ccgacgccgc actcgaacag gtgcgccagg
      781 cgctggcggc ggaggtgccg atctaccccg agctcgagaa ggtgcgcctc tcgttctcgc
      841 tcgagcgcat gcgcgagtac ctcgggcccg accaccccgt cgtcaagcgg gtgctcgggc
      901 ctgcgacgcc cgacgagcgc gcccgcgagc tgatcgccgg atcgacgctc gcctcgccca
      961 aggcccgcct cgcgctcttc gagggcggcc aggcggccgt gaacgcaagc aaggacccga
     1021 tgattgccct cgcgaaggcg gtagacgcgg aagcgcgcgc cctgcggaag gtgtacgagg
     1081 aacaggtgca ggtgcccgag cagcgggcgc agcaggcgat tgccgacgcc cgcttcaagg
     1141 tctacggcac ctccatctac ccggacgcca ccttcaccct gcgcctgtcg tacggctcgg
     1201 tccaggggtg gaacgagtcc ggccggcccg tcgacccgtt cacgcagctc tcgggactgt
     1261 tcgagcgcgc caccggggcg ccccccttcg cgctgccgaa ggcgtggctc gaggcgaagc
     1321 cccgcctcga catgaccacc cgcgccaact tcaccaccac caacgacatc gtcggcggca
     1381 actcgggcag cccggtgatc aacgccgccg gcgagatcgt ggggctgatc ttcgacggca
     1441 acatccactc gatctcgggc ggtttctggt acgacgagga gatgaaccgc gcggtggccg
     1501 tccacccggc gttcatccgg accgcgctcg agaacgtgta caaggtcccg ggcatcgcgg
     1561 gcgagttggg tatccaggca acggtcaaga acacggcgcg gtaggcaagg ccatcctacc
     1621 tcaggcgtgg gcgttccggt gctatcttac gtgctatctt acgtgtgtca tcttacgtgt
     1681 gtcatggctg tctggctgcg atacctgttc gccttcctgt tgttcgctca cggcttcatc
     1741 tacatccgcg tcggcggggc gctgcccggt acggtcaagg gctggaacgg ccggccgtgg
     1801 ctgctcagca aggccatcac cggcggcgca ctcatcaccc tgctgcgtgc cgttcacgtc
     1861 gcggcgggca tcgccatcgt cgcctgcgcg atcgcgatcg cgctcaacca cccatcgtgg
     1921 cgcaccttcg cgatcctcgg gtgcgccgtc gggctggtgg cgttcgcgat cttctgggac
     1981 ggccagacga gcctgctctt cgacgaggga gccgtcggtg cggtgatcag cctcgccctg
     2041 ctgcttaccg cgatcctggc ctgacgctgc cgccagcctg acgctcaccg gctccctttt
     2101 tctcgggtcc gctccttgct tcatcgactt gggcccgggt ggcccagcac atcggcacat
     2161 cggcctaacg gcttgcctcg cttgccctta gacagcttgc ggggcgatcg ctgaaggcat
     2221 tcggctgcat gttccgaaga cactcggggc gtttggcccc gtcgccggcc ccgacccgca
     2281 gcctggctat aaggaggttc cctgatggca accggcacgt cgatgtcccc ggacaaggcc
     2341 gcgctcgggc cgcggcagcg tttcctcgac accctggagc gcgagcacga gacgacgatg
     2401 aaggtgctgc gcgcgtaccc cgccgacagg ttggacctgc ggccgcaccc gcggtgcaac
     2461 accgcccgcg acctgatgtg gacgctggtg atggagcaga agctggcgca gaaggcgttg
     2521 acgatcggct tcgactggtc caaggccccg ggctcgccgc cgccgccccc gcagcagctc
     2581 gacgagatca tcgacacgct cgaacgggag caccgccgag tcgtcgcgct ggtgaaggat
     2641 ctgccggacc agaagctgca cgagaccgtc acgttcccga cggcgcccaa gacgatgggc
     2701 cagtggccga tcgaccagtt cctctggatg ctgctgcacg atcagatcca ccaccgcggg
     2761 cagctgtcga tctacctgcg gatggcggac ggcaagctgc cgtcgatcta cggcccgacg
     2821 gcggacgagc catggctctg acgccggtca aggctcacgg ctcaggactc aaggctcagg
     2881 ggctggcaag ccgcagtctt cagcggcctg ctaacccctc ctccagcatc cagatgtcgc
     2941 cgccgcgcgg cacgaagcgg tcgaacgacg cgagcgtccc gtcgggcgac cacaccgggt
     3001 agtcgatccg cacctcggga tcgtcgaact cgaacaccgc ctcgggcgcg ccctgcccga
     3061 ccctcgacac gtgcagcacc ttgtggttca ccacgtcgag gacgagcgac tcgtcgggcg
     3121 acatcgacat gtgcgccccc ccggcaatct cgcccagggg ctctgcctct cccccctcca
     3181 ccggcacgcg cagcgtcaac ggcttccctc cccccgggca gcggaagatc acgtagcggc
     3241 cgtcgcgggt ccaccggaga aaatgtccca tcgcgccgac ggtggtgagc ggccgcggct
     3301 ccgagccgtc ggcgttcatc acccacacct cgaggcggcc gagccggtcc gactggtagg
     3361 cgatgagccg cccgtcgggc gagtagaacg ggttgatctc gatgccgccg cgcggcgaga
     3421 gcgatgcgag gtcgcccgac tcgaggttta ccgtccagat gccggggctc cccgagcgcg
     3481 acgagaagaa agccagcgtg cgcccgtctg gcgaccagtt cggctggaag tcgccgcccg
     3541 gcccgcgcgt cacctgcttc gacgccgctg ccgccacgt
//
LOCUS       DAOPQX010000382         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_28278_length_3578_cov_3.217428, whole genome shotgun sequence.
ACCESSION   DAOPQX010000382 DAOPQX010000000
VERSION     DAOPQX010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28278_length_3578_cov_3.217428"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PKK95_15415"
     CDS             <1..879
                     /locus_tag="PKK95_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19656.1"
                     /translation="LDDLAPATLTVSEGSAGFAANRRVATSKGYEIGYNPSGPVDHRV
                     PVLRVNGADGKLRAILFGYGCHNTAIGPESYLIGGDYAGAAQQRLERAHPGATALFLQ
                     LAAGDQDPYPRGATEWADRHGATLAAAVEQALASGRQTPLGGPLGTAYRDVSLALAPH
                     TRKLFEQRLADPLPARVRNARTMLKAYDEGRPMVSLPYPVQAVRLGAGFAIVAIGGEP
                     VVDYALRARRELPVVVLAGYSNSVRGYVPSARVLAEGGYEAGDSAIYYGLPGPFAAGV
                     EDTIFTAIRDVLNAAR"
     gene            complement(907..2085)
                     /locus_tag="PKK95_15420"
     CDS             complement(907..2085)
                     /locus_tag="PKK95_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC19657.1"
                     /translation="MSDVKARFGRPLRLALVGGGPASWIGRMHRSAALLDGWWELVGG
                     VFSGDPVRSRTAGAELGFAPERCYPDVGAMIAAETAREDGADAVAIMSPNDTHYPAAA
                     AALDAGLDVIGDKPVSHDFAQACDLVERTRRKGRLFAVVHGYSAYPMTRYARWLVKDG
                     AIGEPRFVQVEYLQGSNATRVEDGPSNRRLEWLFDPARSGLALVMSAIGCHAQHLASV
                     TIGRQVARVSAEVGALVPGRRVVDHVSALLEFEGGARGTFTATNAAAGAENDVRLRVV
                     GERGLIEWWHREPSYLRLAMQGEPLRVVGRGDPYLPGEVIAGGRSPRGHPEGMLQGFA
                     NIYGEVAQERMARSLGDPVPDFLYPRIEEAAHTMAFIEACLASHKSGGWATVAALPAS
                     "
     gene            complement(2097..>3578)
                     /locus_tag="PKK95_15425"
     CDS             complement(2097..>3578)
                     /locus_tag="PKK95_15425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOC19658.1"
                     /translation="QFLGAAGTAAATGLVRAARLAAAESPPNIVLIVADDLGYSDLSC
                     YGQKRFDTPNVDRLAAEGARFTAHYSGSPVCAPSRCSLLTGLHTGHACIRANDELPER
                     GDVWNDPSLEGQRPLPPGTATIATLLKGAGYDTALVGKWGLGGPASDGVPTRHGFDHA
                     FGYLCQRQAHNHYPDHLWRNGRRVALDNPSFRAHQRFDAAADPADPRAYDRYQGRVYA
                     QDAMMDEAIAWLRDRGTRPFFLHLAPALPHLALQVPEEGLAPFAGRFPETPYLGEKGY
                     LPHRTPRAAYAAMIARLDAGVGRLMRTLAETGLDSRTLVIFTSDNGATFEAGGYDGRF
                     FESNAPWRGGKQDLYEGGIRVPFIARWPGRVPKGIVRDEPCASWDLAATFADLAGATP
                     MPGHDGVSLRPLLEGRPAAPRAPLYWEYLGKQAVRDGRWKAILDAPTGVIEVYDLAAD
                     PGERRNVARARAGEAARLRQIMRDSRVESSLFPLVKARTVPAA"
BASE COUNT          499 a         1312 c         1290 g          477 t
ORIGIN      
        1 ctggacgacc tggctcccgc cacgctgacc gtgtcggagg gctcggcagg ctttgcggcg
       61 aaccggcgcg tggccacttc gaaagggtac gagatcggct acaacccgtc ggggccggtc
      121 gatcaccggg tgccggtgct gcgggtgaac ggcgccgacg gaaagcttcg cgccatcctc
      181 ttcggctacg gctgccacaa caccgcgatc gggcccgagt cgtacctgat cggcggcgac
      241 tacgcggggg ctgcacaaca gcgcctcgag cgcgcgcacc ccggtgcgac cgcgctgttc
      301 ctgcagctcg ccgccggcga ccaggacccc tacccgcgcg gtgcgacgga gtgggccgac
      361 cggcacggcg cgacgcttgc tgccgcggtg gagcaggcgc tcgcctcggg gaggcagacg
      421 ccgctcggag ggccgctcgg aaccgcctac cgggacgtct cgctggcgct cgcgccgcac
      481 acgcggaagc tgttcgagca gcggctcgcc gatccgctgc cggcccgcgt ccgcaacgct
      541 cgtacgatgc tgaaggccta tgacgagggc cggcccatgg tgtcgctgcc gtacccggtg
      601 caggcggttc gcctcggggc cggcttcgcc atcgtggcca ttggcggcga gcccgtcgtg
      661 gactacgcgc tccgcgcccg ccgcgagctg ccggtggtcg tgctcgccgg ctactcgaac
      721 agcgtgaggg gctacgtgcc gagcgcacgg gtgcttgccg aagggggata cgaggccggg
      781 gacagcgcga tctactacgg ccttcccggg ccctttgcgg ctggcgtcga ggacacgatc
      841 ttcaccgcga ttcgagacgt gctgaacgcg gcgcggtgac cggccggccg ccgcgccgcg
      901 tgacgctcac gacgccggta gcgcggccac cgtcgcccag ccgccgctct tgtgcgacgc
      961 gaggcacgcc tcgatgaacg ccatcgtgtg tgcggcttcc tcgatgcgcg ggtagaggaa
     1021 gtcgggcacc gggtcgccca gcgaccgggc catccgctcc tgcgccacct cgccgtagat
     1081 gttggcgaac ccctgcagca tcccctcggg gtgcccgcgc ggcgaccgcc cgcccgcgat
     1141 cacctcgcct gggaggtacg gatcgccgcg cccgaccacg cgcagcggct ctccctgcat
     1201 cgcgagccgc aggtagctcg gctcgcggtg ccaccactcg atcagcccac gctcgccgac
     1261 cacccggagc cgcacgtcat tctccgcgcc ggccgctgca ttggtggctg tgaaggttcc
     1321 gcgggcccct ccctcgaact cgagcagggc cgacacgtga tcgacgacgc gccggccggg
     1381 aacgagcgcg cccacttccg ccgagacccg ggccacctgg cggccgatgg tgaccgaggc
     1441 caggtgctgc gcgtggcagc cgatcgcgct catgacgagc gcgaggccgc tgcgcgccgg
     1501 gtcgaacagc cactcgaggc ggcggttcga cgggccgtcc tccacccggg tcgcgttgct
     1561 tccctgcagg tactcgacct gcacgaagcg gggctcgcca atcgcgccgt ccttcacgag
     1621 ccagcgggcg tagcgcgtca tcgggtaggc ggaatagccg tggacgaccg cgaagaggcg
     1681 gcccttgcgg cgcgtccgct cgaccaggtc gcacgcctgg gcgaaatcgt gcgagaccgg
     1741 cttgtcgcca atcacgtcga ggcccgcgtc gagggcggcc gcggcggcgg ggtagtgcgt
     1801 gtcgttgggc gacatgattg ccacggcatc cgcgccgtcc tcgcgcgcgg tctcggccgc
     1861 gatcatcgcg cccacgtccg ggtagcagcg ctcgggcgca aagcccagct cggcacctgc
     1921 ggttcgcgag cggaccgggt cgccggagaa cacgcccccg accagttccc accagccgtc
     1981 gagcagcgcc gccgaccgat gcatccggcc aatccacgag gccggcccgc cgccgaccag
     2041 tgccaggcga agcggacggc cgaagcgcgc cttcacgtca ctcacgattc cccctctcaa
     2101 gcggccggca cggtgcgggc cttcaccagc gggaacaacg acgactccac cctcgagtcg
     2161 cgcatgatct gccgcagcct cgcggcctcg ccggcccgtg cccgcgccac gttccgccgc
     2221 tcgcccgggt cggccgcgag atcgtacacc tcgatcacgc cagtcggtgc atcgaggatc
     2281 gccttccacc gcccgtcgcg caccgcctgc tttccgagat actcccagta gagcggcgcc
     2341 cgcggggcgg caggccggcc ctcgagaagc ggccgcagcg acaccccgtc gtgcccgggc
     2401 atcggcgtcg cgccggcgag gtcggcgaag gtggcggcga ggtcccacga ggcacagggc
     2461 tcgtcgcgga cgatgccctt cggcacccgc ccgggccagc gcgcgatgaa ggggacgcgg
     2521 atgccccctt cgtagagatc ctgcttgccg ccgcgccacg gcgcgttgct ctcgaaaaac
     2581 cgcccgtcgt agccgcccgc ctcgaaggtc gccccgttgt cgctggtgaa aatcaccagc
     2641 gtgcgcgaat cgagacccgt ctcggcgaga gtccgcatca accggccgac cccggcgtcg
     2701 aggcgggcga tcatggccgc gtaggcggcg cgaggtgtgc ggtgcggcag gtatcccttc
     2761 tccccgaggt acggagtctc ggggaaccgg ccggcaaacg gggccaggcc ctcctcgggg
     2821 acctgcagcg ccaggtgcgg cagggccggg gccaggtgca ggaagaaggg gcgcgtgcca
     2881 cggtcgcgca gccacgcgat cgcctcgtcc atcatcgcat cctgggcgta tacccgcccc
     2941 tggtagcgat cgtaggcgcg cgggtcggcg gggtctgcgg ccgcgtcgaa cctctggtgc
     3001 gcgcggaacg aagggttgtc gagggcgacg cggcgcccgt tccgccagag gtgatcggga
     3061 tagtggttgt gggcctgccg ctggcagaga tacccgaagg cgtggtcgaa gccgtggcgc
     3121 gtcggcacgc cgtcgctcgc cggcccccca aggccccact tgccgaccag cgccgtgtcg
     3181 tagccagccc ccttgagcag cgtggcgatg gtcgcggtgc cgggaggaag cggccgctgc
     3241 ccctcgagcg acgggtcgtt ccagacgtcg ccgcgctcgg gcagttcgtc gttcgcgcgg
     3301 atgcaggcat ggccggtgtg caggccggtc agcagcgagc agcgcgaggg ggcgcacacg
     3361 ggactgcccg agtagtgcgc cgtgaaccgg gcgccctcgg cggcgagcct gtcgacgttc
     3421 ggcgtgtcga accgcttctg cccatagcag gagaggtccg agtagccgag atcgtcggcc
     3481 acgatcagca cgatgttggg cggggactcc gcggcggcaa gcctcgcggc ccgaacgaga
     3541 cccgtcgcgg cggcggtccc ggccgcgccg aggaactg
//
LOCUS       DAOPQX010000383         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_28765_length_3531_cov_6.510644, whole genome shotgun sequence.
ACCESSION   DAOPQX010000383 DAOPQX010000000
VERSION     DAOPQX010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28765_length_3531_cov_6.510644"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKK95_15430"
     CDS             <1..293
                     /locus_tag="PKK95_15430"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOC19659.1"
                     /translation="PCEYRVAPASVTVAAEGGEVALSLATGSSCAWQASDDAAWVRVG
                     PPDSGTGPAAIPVAIEANTGQAARRATVQVASASVALVQEGAPVLSFTLFRS"
     gene            complement(542..1339)
                     /locus_tag="PKK95_15435"
     CDS             complement(542..1339)
                     /locus_tag="PKK95_15435"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOC19660.1"
                     /translation="MYRRHFGLTGHPFSPEIAPDDLFLSSPARELEVRLTHLLDLRGI
                     GLVTGDAGSGKTCACRRVLARLHTGLYRVIYVSLSTGNVMDLYKTISWEMGLAVERSR
                     AALYRQIRTEVSRLCTDARIRPVLVVDEAHHLRADVLEDLRLLTNYQMDAENRLCLLL
                     LGQTELRRRLTMAVHEALAQRIVIRYHVTPLSREELPAYLAHRLQLAGATVPLFDATA
                     EEALYQATSGLPRKINLLAHHTLMAATLTKAKVATADHLQAALPEVA"
     gene            complement(1341..2672)
                     /locus_tag="PKK95_15440"
     CDS             complement(1341..2672)
                     /locus_tag="PKK95_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOC19661.1"
                     /translation="MPHRTDATTHAETVALFRYGLIADLRDLRPGDRSLHTRLEEKAA
                     REYDIPGTTRRHVAPGTMRDWLYAYRRGGFDALKPRPRRDVGRARALPQAVADALCAL
                     KEEAPTFSVLMLIATARQRGLIPADAEVAPATVHRLLTRQGLMARAPETPTSKDRRRF
                     AFALANDLWMSDVMHGPTVFEEDRRRHKTYLIALLDDATRVVPHAAFARSENVAAFLP
                     VFARAIQRRGVPSRLYVDNGSAYRARHLALVCAKLGVTLIHARPYQPQAKGKIERFFR
                     TCRAQLLPTLLEADTRSLDALNRRLWAWLEDEYHETPHRGLEGETPLARWAATAGGLA
                     LPPEQLDELFLFEEKRKVHKDRTVSLRGVVYEVEASLVGEMVTLRYDPTRPTRGVTVV
                     FRDRVVDVAKPVDAYANCFVRRDHQTHNLTTTKPAAAPPAGLPLRRLDEDV"
     gene            complement(2714..3175)
                     /locus_tag="PKK95_15445"
     CDS             complement(2714..3175)
                     /locus_tag="PKK95_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19662.1"
                     /translation="MRVTRYYCPTAHETFSLLPDCLASRFPSALDDLEHVVTQVTAAR
                     SVEAAADRLRPDIELPSAVRWIRRRLTLVRASLVIAAGVVGLALADVTLETLRAAWQT
                     DRVLVALRGRMAVHLGQLPPPIGFGRRPISRPVRRPGRQHAMGPDRRGPSG"
BASE COUNT          509 a         1113 c         1379 g          530 t
ORIGIN      
        1 cgccttgcga gtaccgcgtg gcacccgcat cagtcaccgt tgcggccgag ggcggcgagg
       61 ttgcgctgtc gctcgcgacc ggctcctcgt gcgcctggca ggcatctgac gatgccgcgt
      121 gggtgagagt ggggccgccc gactcgggca ccgggccggc ggcgatcccg gtggcgatcg
      181 aggcgaacac cggccaggcg gcacggcgcg cgacggtcca ggtggcgagc gcgagcgtgg
      241 cactcgtgca ggagggcgcg ccggtgttgt ctttcacgtt gttccgaagc tgattcctca
      301 cgctctttcc tgattacgtg atcccggttt ctcagacttg acccgctggg ccttgagggc
      361 cgagcgtgag ctgcggtcgc tgcgccatgc gcaggacgcg ggtgcgttta ccgcttgggc
      421 tcccgccagg acgaaggcgg gggccgccgg tcaggcggcc cctcgtcctg cgcgagtccg
      481 tcggggcgct cgggtcgctc gccagcggag ccctatcctc cccgacggcg aggcgcgtcg
      541 gtcaggccac ttcggggagg gcggcctgga gatggtcggc cgtggcgacc ttggctttgg
      601 tgagggtggc ggccatcagg gtgtgatgcg ccaggagatt gatcttgcgg ggcaggccgg
      661 aggtggcctg gtagagcgcc tcttcggcgg tcgcgtcgaa gagggggacg gtggcgccgg
      721 cgagctgaag gcgatgggcg agataggccg gtagctcctc gcggctgagc ggcgtgacat
      781 ggtagcggat gacgatccgc tgcgcgaggg cctcgtgcac ggccatggtg agccggcggc
      841 ggagctcggt ttggccgagg aggagcagac acagacggtt ctccgcatcc atctgatagt
      901 tggtgagcag gcgcaggtcc tcgaggacgt cggcgcggag gtgatgcgct tcatcgacga
      961 ccagcaccgg acggatgcgg gcatcggtgc agaggcggga gacctcggtg cggatctgcc
     1021 gatagagcgc ggcgcgggag cgttcgaccg cgaggcccat ctcccaggag atggtcttgt
     1081 acaggtccat gacgttgccg gtggagaggg acacgtagat cacgcggtag agtccggtgt
     1141 ggagccgcgc gaggacgcgg cgacacgcgc aggtcttgcc gctgcccgcg tcgccggtga
     1201 cgagcccgat gccgcgcagg tccaggaggt gcgtgaggcg cacctcgagc tcgcgggcgg
     1261 gactggagag aaacaggtcg tcgggggcga tctcgggact gaagggatgg ccggtcaggc
     1321 cgaagtggcg acggtacatc ttagacatcc tcatcgaggc ggcgcagggg caaacccgcc
     1381 gggggcgcgg ccgccggctt ggtggtcgtg aggttgtgcg tctggtgatc gcgccggacg
     1441 aagcagttgg cgtaggcgtc gacgggcttg gcgacatcca ccacgcggtc gcggaagacg
     1501 acggtgaccc cgcgcgtggg gcgggtcggg tcgtaacgca aggtcaccat ctccccgacc
     1561 agcgacgcct cgacctcgta gacgacgccc cggaggctga cggtgcggtc cttgtggacc
     1621 ttgcgcttct cctcgaagag gaagagctcg tcgagctgct cgggcgggag cgcgagcccg
     1681 ccggcggtcg cggcccagcg ggcgagcggg gtctcaccct cgaggccccg gtgcggcgtc
     1741 tcgtggtact cgtcttcgag ccacgcccag agccggcggt tcagcgcgtc gaggctgcgc
     1801 gtgtcggcct cgaggagcgt ggggaggagc tgggcccggc aggtccgaaa gaatcgttcg
     1861 attttgccct tcgcctgggg ctggtagggt cgggcgtgaa tcagggtgac ccccagcttc
     1921 gcgcacacca gggccaggtg gcgggcgcga tacgccgatc cgttatcgac gtagaggcgc
     1981 gagggcacgc cccgacgctg gatcgcgcgg gcgaagacgg gcagaaacgc agcgacgttt
     2041 tcgctgcgcg cgaaggccgc atgggggacg acgcgcgtcg cgtcgtcgag cagcgcgatg
     2101 aggtaggtct tgtgacgccg ccggtcctct tcgaagacgg tgggcccgtg catgacgtcg
     2161 ctcatccaga ggtcgttggc gagcgcaaac gcgaagcgcc ggcggtcctt gctggtcggc
     2221 gtctccggtg cgcgggccat caagccctgc cgcgtgagca accggtgcac ggtggccggc
     2281 gcgacctcgg cgtcggcggg gatgagtcca cgctgccggg cggtcgcgat cagcatcagc
     2341 acgctgaagg tcggcgcctc ctccttcagg gcgcacaggg cgtcggccac cgcctggggc
     2401 aaggcgcggg cgcgcccgac gtcgcgccgc ggcctcggct tgagcgcgtc gaagccgccg
     2461 cgacggtagg cgtagagcca gtcgcgcatc gtcccgggcg cgacatggcg gcgcgtggtc
     2521 cccgggatgt cgtactcgcg ggcggccttc tcctcgaggc gggtgtggag cgagcgatcg
     2581 ccgggccgca ggtcgcggag atcggcgatg aggccgtagc gaaagagcgc gacggtctcg
     2641 gcgtgggtgg tggcatccgt tctgtgcggc atcggccctc cttgaccgcg gtcgcggcgg
     2701 tcaggcggga tcctcagccg gacgggcccc tgcggtcagg ccccatcgcg tgttggcggc
     2761 ctggccgccg aacgggacgc gagatcggac gacggccgaa tccgatcggc ggcgggagct
     2821 ggccgagatg gacggccatg cggccgcgca gtgcgacgag gacgcggtcg gtctgccacg
     2881 ccgcgcggag cgtctcgagt gtcacgtccg ccaaggccag gcccacgaca ccggcggcga
     2941 tcacgaggga ggcccgcacg agcgtgagcc gacggcggat ccagcggacg gccgatggca
     3001 gctcgatgtc cgggcgcagc cggtcggctg cggcttcgac actgcgcgcg gcggtgacct
     3061 gcgtcacgac gtgctcgaga tcatcgaggg cactgggaaa ccggctggcc aggcaatccg
     3121 gaaggaggct gaaggtctca tgggccgtcg ggcagtagta ccgcgtgacg cgcatccccg
     3181 tgggcgtctt gcgggggtac gtcccgtgac ggaccaaccc gcacccaccc gccggatggc
     3241 gcggacacgt ggtcagcctt gccttcaccc aggcgtgttc ggcaagatag gcctcgctgg
     3301 acagcggtgt ctcgtgacgc agctgcaccg gcctgcgcac ggcacgggtg gctccgtggc
     3361 gtgtgcagcc tcccttcccc gtcgctgctc aacccgacgg cggggaaggt tcttatcaca
     3421 gctcgtccct cggccgcagc gcacactgcg ccctgcgatc caccgattcc tcagcgggcc
     3481 aatccgagaa gaactgtcag agggattgcg gtactacgtg agagctaaca g
//
LOCUS       DAOPQX010000384         3530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_28779_length_3530_cov_3.513669, whole genome shotgun sequence.
ACCESSION   DAOPQX010000384 DAOPQX010000000
VERSION     DAOPQX010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28779_length_3530_cov_3.513669"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..720)
                     /locus_tag="PKK95_15450"
     CDS             complement(187..720)
                     /locus_tag="PKK95_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOC19663.1"
                     /translation="MAQAKEQAVHGGARTAEGQLTAENTADSPIVQGLKAQVANAFLL
                     YLNYKHYHWQTFGPHFRDLHRLFDEFAEEVLAGIDPLAERIRMIGQDPPARPNELADL
                     ASVSIAAPHSNMREMVEEGRQNLIGVIKQMREAAKTADDRDDPGTVDILSKTVQVYEK
                     HEWWLRDILKKDDGLGD"
     gene            904..1788
                     /locus_tag="PKK95_15455"
     CDS             904..1788
                     /locus_tag="PKK95_15455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOC19664.1"
                     /translation="MDSCLIVNPVSGSDRAPEFLPAIEERLRSTFGGLDVKVTSHEGD
                     AEDTARQAAETGCRRVVVAGGDGTLNEALNGIASAGALDRVAIGLLPLGTGNDFAGTL
                     GLHDDPLAAAEVVASGRERRVDVGVVNGRVFVNASAGGFLAEVSDALTPGMKTIAGRL
                     AYLLAGAGVIASFDPPSAAVTIGGESLGARQYQLFVVCNGPTIGGGHRAAPRARPDDG
                     LLDACLVEASGTLDLVALLRRLSKGEHLDAEGVTYRQFQSAELAFDRVLKVNTDGEVI
                     ETRICRYRVLPGAARFIA"
     assembly_gap    1876..1975
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1976..3377
                     /locus_tag="PKK95_15460"
     CDS             <1976..3377
                     /locus_tag="PKK95_15460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOC19665.1"
                     /translation="FSTSTFSTSTFSTSTFSTSSTSTPSTSCTPAPSAPTLTPLPSLD
                     QTRGVQPFHLLIKPTGADCNLGCRYCFFRPKASLYPGSSFRMTDEVMEAAIEQLLAAH
                     PGPDVAFGWQGGEPTLMGLSFFERVMEAIERHRRPGQRVSQSIQTNGILVNDEWAAFL
                     ARHRFLVGLSLDGPPALHDAFRVDAGGHGTHGQVMRAWEALARHGVEVNVLCSVHAAN
                     ASQPLAVYRFFRDRLSARFVQFIPIVERAGGQGAAVSARSVRPGEYGRFLATVFDEWV
                     CRDVGTVFVQAFDAALAGWIGQPSLCVFAPTCGRAPVLEHNGDLYACDHFVDPAHLVG
                     NILEERLETLLDSQALREFGEDKRRGLPAACLACDVVFACHGECPRNRFGIGRDGGHG
                     PNYLCEDYRRFFRHVAPSMEAMVNLLGRGRAPAEIMRLGVAGPAGLASRLAAAGRNDP
                     CPCGSGLKTKRCHGGR"
BASE COUNT          467 a         1230 c         1177 g          556 t
ORIGIN      
        1 atctcgcagt ggaaggaggt cggtcgcgcc caccgcgagt tcttcggcga gatccgtccg
       61 tgcgcgacga tggtcgaggt cagccggctg gtcgaccccg acatgctcgt ggaaatcgag
      121 gccgacgcga tcatcgagtg agccgggcgg ggccggccgg cgagccggcc ggccccgcct
      181 gcgacgtcag tcgccgaggc cgtcgtcctt cttgaggatg tcgcgcagcc accactcgtg
      241 cttctcgtag acctgcaccg tcttcgacag gatgtcgacg gtgccggggt cgtcccggtc
      301 gtcggccgtc ttggccgcct cccgcatctg cttgatcacg ccgatcaggt tctgcctgcc
      361 ctcctccacc atctcgcgca tgttcgagtg cggtgccgca atcgacaccg acgcgaggtc
      421 cgccagctcg ttcggccggg cgggcggatc ctgaccgatc atccggattc tctcggctaa
      481 cggatcgatg cccgcgagca cctcctcggc gaactcgtcg aacagccggt ggaggtcgcg
      541 gaagtgcggc ccgaaggtct gccagtggta gtgcttgtag ttgagataca gcaggaacgc
      601 attggccacc tgggccttga gcccctgaac gatcgggctg tcggccgtgt tctcggccgt
      661 gagttgcccc tcggcggtgc gtgccccgcc gtgaaccgct tgctccttcg cctgtgccat
      721 cggtttcgct cctgtgtgta aggtcgatcc cggccctgct tccgagggga agcccggggc
      781 gaacgatgca agaacgggtt cagcacgcac ggccgagccc ggccggctcc ttgctaatgg
      841 tcgcctccca ggggccaccc ttgtggtcgc cctcgtgaag cgaagccttc ttggaggccg
      901 ccgatggaca gctgcctgat cgtcaacccc gtgtccggct cggatcgggc gcccgagttc
      961 ctcccggcca tcgaggagcg cctgcggagt acgttcggcg gcctcgacgt gaaagtcacc
     1021 tcgcacgaag gcgatgccga ggacacggct cgccaggccg ccgagacggg gtgccgccgt
     1081 gtggtggtgg cgggcggcga cgggacgctg aacgaggcgc tgaacgggat cgcatcggcc
     1141 ggcgcgctcg accgggtcgc catcggcctg ctccccctgg gcaccggcaa cgacttcgcc
     1201 ggcacgcttg ggctgcacga cgacccgctc gccgccgccg aggtcgtggc gtcggggcgc
     1261 gagcggcgcg tggacgtcgg cgtggtcaac ggccgggtct tcgtgaacgc gtcggccgga
     1321 gggttcctgg ccgaggtgtc ggacgccctc acgcccggca tgaagacgat tgcggggcgg
     1381 ctcgcctacc tgcttgccgg cgccggcgtg atcgccagct tcgatccgcc ttccgccgcg
     1441 gtcaccatcg gcggcgagtc gctcggtgcc cgccagtacc agctgttcgt cgtctgcaac
     1501 gggcccacca tcggcggcgg ccaccgtgcg gctccgcgcg cccgacccga cgacgggctg
     1561 ctggacgcgt gtttggtgga agcgtcgggc acgctcgatc tggtcgccct gctccggcgg
     1621 ctgtcgaagg gcgagcacct cgatgcggaa ggggtgacct accggcagtt tcaatccgcc
     1681 gagctggcct tcgaccgggt gctgaaggtg aacaccgacg gcgaggtgat cgaaacgcgc
     1741 atctgccgct accgcgtgtt gcctggagcc gcccgattca tcgcctgatg tggcgaaggg
     1801 cgcaggccct tcagcacctc cagcaccagc accttcagca ccagcacctt cagcaccagc
     1861 accttcagca ccagcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncttca
     1981 gcaccagcac cttcagcacc agcaccttca gcaccagcac cttcagcacc tccagcacca
     2041 gcacccccag cacctcctgc acccctgcac cttcagcacc taccttgaca ccactccctt
     2101 ccctcgacca gactcgcggc gtgcagccgt ttcacctcct gatcaagccg accggggctg
     2161 actgcaacct cgggtgccgc tactgcttct tccgccccaa ggcgtcgctg tacccgggta
     2221 gctcgttccg gatgaccgac gaggtgatgg aggcggcaat cgagcagctg ctggccgcgc
     2281 acccggggcc cgacgtcgcg ttcgggtggc aggggggcga gccgaccctc atggggcttt
     2341 ccttcttcga gcgcgtgatg gaggccatcg agcggcaccg ccgccccggg cagcgcgtct
     2401 cgcagtcgat ccagaccaac ggcatcctgg taaacgacga gtgggcggcg ttcctcgcgc
     2461 ggcatcgctt cctcgtgggc ctcagcctcg atggtccccc ggcgcttcac gacgccttcc
     2521 gcgtggacgc gggtggccac gggacccacg gccaggtgat gcgcgcgtgg gaggcgctcg
     2581 cgcggcacgg cgtcgaggtg aacgtcctct gcagcgtgca cgcggccaac gccagccagc
     2641 cgctcgccgt ctaccggttc ttccgcgacc gcctctccgc ccgcttcgtg caattcatcc
     2701 cgatcgtgga gcgtgccggc gggcaggggg cggccgtgag cgcccggtcg gtccgtcccg
     2761 gcgagtacgg ccgcttcctg gcgacggttt tcgacgagtg ggtctgccgc gacgtcggga
     2821 ccgtcttcgt ccaggcgttc gacgcggcgc tggccggctg gatcggccag ccgtcgctct
     2881 gcgtcttcgc accgacgtgc gggcgcgcgc cggtgctcga gcacaacggc gacctctacg
     2941 cgtgcgacca cttcgtcgac cccgcgcacc tggtcggaaa catcctcgag gagcgcctcg
     3001 agacgctgct cgactcccag gcgctgcgcg agttcggcga ggacaagcgg cgcggcctgc
     3061 ccgcggcctg cctggcgtgc gacgtggtgt tcgcctgcca cggcgagtgt ccgcgcaacc
     3121 gcttcgggat cggccgggat ggcgggcacg gaccgaacta cctgtgcgag gattaccgcc
     3181 gcttcttccg gcacgtcgcc ccttcgatgg aggcgatggt gaacctgctc ggccgcggcc
     3241 gggcgccggc ggagatcatg cggctggggg tggccggccc cgccggactg gcctccaggc
     3301 tggcagccgc cgggcgtaac gatccctgcc cgtgcggcag cgggctcaag accaagcgat
     3361 gccacggggg acgatgatgg aacgggaggc gtggacggcg gtgacgcgcc ggcagttcct
     3421 cggcgcggcc gggaccgccg ccgcgacggg tctcgttcgg gccgcggggc ttgccgccgc
     3481 ggagcccccg cccaacatcg tgctgatcgt cgccgacgat ctcggctact
//
LOCUS       DAOPQX010000385         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_29016_length_3510_cov_4.500724, whole genome shotgun sequence.
ACCESSION   DAOPQX010000385 DAOPQX010000000
VERSION     DAOPQX010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29016_length_3510_cov_4.500724"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="hemW"
                     /locus_tag="PKK95_15465"
     CDS             complement(<1..1011)
                     /gene="hemW"
                     /locus_tag="PKK95_15465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOC19666.1"
                     /translation="MEPMPPPSPLGLYLHVPFCASICGYCNFNRGLFDPALKAAYVEA
                     IRLEIRRAADGAAADTIYFGGGTPSLLEPAEVGAIIGECRAAFAVAAGAEITLEANPE
                     TVTRASLAAFREHGVNRISFGVQSFRDEELRRLDRAHSAGRARLAFADARAAGFENLS
                     LDLMLWLPGQTCAHVEESLDGLLALGPEHASVYLLELYPNAPLRETMARAGWSQAPDD
                     DAADMYLMAMERLEEGGLEQYEISNLAAPGRRCQHNLKYWTDGEWAGFGPGAHSTRHR
                     VRWNNVSGTLEYTRRIEAGEPVVASRRLLGPREALEEALFMGLRLRDGVSCEALGARY
                     GVD"
     gene            1071..2078
                     /locus_tag="PKK95_15470"
     CDS             1071..2078
                     /locus_tag="PKK95_15470"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOC19667.1"
                     /translation="MPEHYYSRETVALRAELGQSLDRSRLRALHQRRAARHLVVGARQ
                     FGLLAACGWGLAVFEHPLIWLPLAVVQGFTIFNFTVLLHEVVHNNVFERPRPRAERLL
                     GWLYAVPSGISASQFTRWHLDHHAELGSEEDDPKRHFLSPKINARWFKLLYCTPALFP
                     IYFRAARLAAAGYDERLRRTIARERWVAILVHLGALAAFWVWGGLPVALRAYVVPVFF
                     VFPLAFTLNRLGQHYDIEPDDPAGWTTLVRGHWFWNAVFLNSNFHLEHHYFPGVPFYN
                     LPALQRELTPFYLRRGLRWRDYGSLVRGWLLENRAPHTRWDDAAKPLIASGDRAAGPT
                     R"
     gene            2087..3226
                     /locus_tag="PKK95_15475"
     CDS             2087..3226
                     /locus_tag="PKK95_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOC19668.1"
                     /translation="MDFRPTDHHQVLRRSIREFAEREIRPHVMEWDEAQHFPEELVPG
                     LAALGLFGIQVPEAYGGAGMSALDYCICIEELARVDPSVALSVAAHNGLCTAHLVMFG
                     SEEQKQRFVPALAQGERIGAWGLTEPLAGSDAGGTRTRAERRGGGWVLNGSKTFTTHG
                     RVGHVLVAMAVTDPKAGRRGISAFVVERGTPGMRPGRKENKLGMRASDTSEVVFEDCC
                     IGGDQLVGREGEGFVNALQVLDAGRIGIAALAVGLAQGAHEAAVAYAKQRHQFGQPIA
                     SFQAIQWKLADDATAIEAARLLTYRAAALKDQGLRTTRESSMAKLFASEMAVRVAENG
                     VQIHGGYGFVKDYPAEKYFRDVKLTTIGEGTSEIQRLLIAREIIG"
     gene            3232..>3510
                     /locus_tag="PKK95_15480"
     CDS             3232..>3510
                     /locus_tag="PKK95_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19669.1"
                     /translation="MLGVQEVPEVPRVLVPKVPRVLVPKVPRVLVPEVLRVRMPKVLP
                     ALPLAVRCAATARRLRRGRPPAPSALHLRHLQHRTRLRRAVRGYGAADL"
BASE COUNT          493 a         1220 c         1278 g          519 t
ORIGIN      
        1 gtccacgccg taccgggcgc cgagcgcttc gcacgacacc ccgtcacgca gccggagtcc
       61 catgaagagc gcctcctcca gcgcctcgcg ggggccgagc aagcggcgcg acgcgacgac
      121 cggctcgccg gcctcgatgc ggcgtgtgta ttcgagggtg ccgctcacat tgttccatcg
      181 aacccgatgc cgtgtcgagt gggcgcccgg gccgaaccca gcccattcgc cgtcggtcca
      241 gtacttcaag ttgtgctggc agcggcgtcc cggcgcggcc aggttggaga tctcgtactg
      301 ctccaggccg ccctcctcga ggcgttccat cgccatcagg tacatgtccg ccgcgtcgtc
      361 gtcgggcgcc tgagaccatc cggcccgcgc catggtctcg cgaagggggg cgttggggta
      421 cagctcgagg aggtacaccg aggcgtgctc gggaccgagg gcgagcaggc cgtcgagcga
      481 ctcctcgacg tgagcgcacg tctgcccggg cagccagagc atgaggtcga ggctgaggtt
      541 ctcgaacccc gccgccctcg cgtcggcgaa cgcgaggcgg gcgcgcccgg ccgagtgcgc
      601 ccggtcgagg cgccgcagtt cctcgtcgcg aaacgactgc acgccgaagc tgatccggtt
      661 gaccccatgc tcgcggaagg ctgcgagcga ggccctggtg accgtttcgg ggttggcctc
      721 gagggtgatc tcggccccgg ccgcgaccgc gaacgccgcg cggcactcgc cgatgatggc
      781 gccgacctct gccggctcga ggagcgacgg tgtgccgccc ccgaagtaga tcgtgtcggc
      841 ggcggcgcca tcggccgccc gccggatctc gagccgaatc gcttccacgt aggcggcctt
      901 cagcgccggg tcgaacagcc cccggttgaa gttgcagtaa ccgcagatcg aggcgcagaa
      961 gggcacgtgc aggtagaggc cgaggggaga ggggggaggc atgggttcca ttccccccat
     1021 gctatagtcg atccaagggc tttctcccaa accgactgga gtcgcggggc gtgccggagc
     1081 attactacag ccgcgagacg gtggcattgc gcgccgagct ggggcagtcg ctcgaccgct
     1141 cgcgcctccg tgcgctccac cagcgccgcg ccgcgcggca cctggtggtg ggggcgcgcc
     1201 agttcgggct gctcgcggcc tgcggctggg gcctggcggt cttcgagcac cccctcatct
     1261 ggctgccgct ggccgtcgta cagggcttca cgatcttcaa cttcacggtc ctcctgcacg
     1321 aggtggtgca caacaacgtc ttcgagcggc cgcgcccgcg cgccgagcgg cttctgggct
     1381 ggctctacgc cgtgccgagc ggcatctcgg cgtcgcagtt cacgcgctgg cacctcgacc
     1441 accacgccga gctgggctcg gaggaggacg atcccaagcg ccacttcctc tcgcccaaga
     1501 tcaacgcccg ctggttcaag ctgctctact gcacgccggc gctcttcccg atctacttcc
     1561 gcgcggcgcg tctcgcggcg gccgggtacg acgagcggct tcggcggacg atcgcacgcg
     1621 agcgctgggt cgccatcctc gtccacctcg gggcgctggc ggccttctgg gtgtggggcg
     1681 gcctgccggt cgcgctgcgc gcctacgtcg tgcccgtgtt cttcgtcttc ccgctggcct
     1741 tcacgctcaa ccggctcggc cagcactacg acatcgagcc agacgacccg gcggggtgga
     1801 cgacgctggt gcgcggccac tggttctgga acgccgtgtt cctgaactcg aacttccacc
     1861 tcgaacacca ctacttcccg ggcgtgccgt tctacaacct gccggcgctc cagcgcgagc
     1921 tgacgccgtt ctacctgcgc cgcggcctgc gctggcggga ctacggcagc ctggtccgcg
     1981 gctggctgct cgagaaccgg gccccgcaca cccggtggga cgacgcggcg aagccgctga
     2041 tcgcctcggg cgaccgggcc gccggtccga cgcgctgacc aacccgatgg acttccgacc
     2101 caccgaccac caccaggtgc tgcgccggtc gatccgcgag ttcgccgagc gggagattcg
     2161 gccgcacgtc atggagtggg acgaggcgca gcacttcccc gaggagctgg tccccgggct
     2221 tgcggcgctg ggcctgttcg ggatccaggt gcccgaagcc tacggcggcg cggggatgtc
     2281 ggcgctcgac tactgcatct gcatcgagga gctggcccgc gtcgatccct cggtcgccct
     2341 ctcggtcgcg gcccacaacg gcctgtgcac ggcgcacctg gtgatgttcg gctccgagga
     2401 gcagaagcag cgcttcgtgc cggcgctggc ccagggtgaa cggatcgggg cgtgggggct
     2461 gaccgagccg ctggcgggaa gcgacgcggg cggaacgcgg acgcgggccg agcggcgcgg
     2521 cggcgggtgg gtgttgaacg gctcgaagac cttcaccacg cacggccgcg tcgggcacgt
     2581 actggtggcg atggccgtca ccgaccccaa ggccggccgc cgcggcatct cggcgttcgt
     2641 cgtcgagcgc gggacgcccg gcatgcgccc cgggcgcaag gaaaacaagc tgggcatgcg
     2701 cgcgagcgac accagcgagg tggtgttcga ggactgctgc atcggcggcg accagctggt
     2761 cgggcgcgag ggcgaggggt tcgtcaacgc cctgcaggtg ctcgacgcgg ggcggatcgg
     2821 catcgcggcg ctggccgtcg gcctggcgca gggggcgcac gaggcggcgg tcgcctacgc
     2881 gaagcagcgc caccagttcg gccagcccat cgcctcgttc caggcgatcc agtggaagct
     2941 ggccgacgac gccaccgcca tcgaggccgc gcggctgctc acctaccggg cggccgcgct
     3001 gaaggaccag gggctccgca ccacgcgcga gtcgtcgatg gcgaagctgt tcgcgagcga
     3061 gatggccgtg cgggttgccg agaacggcgt ccagatccac ggcgggtacg gcttcgtgaa
     3121 ggactacccg gccgagaagt atttccgcga cgtcaagctg acgaccatcg gcgaggggac
     3181 gagcgagatt cagcggttgc tgatagccag ggagatcatc ggctaggtgt cgtgctgggg
     3241 gtgcaggagg tgccggaggt gccgagggtg ctggtgccga aggtgccgag ggtgctggtg
     3301 ccgaaggtgc cgagggtgct ggtgccggag gtgctgaggg tgcggatgcc gaaggtgctc
     3361 ccagcactcc ccttggccgt gcggtgcgcg gctacggcgc ggcggctacg gcgcggcaga
     3421 cctccggcac cttcagcact gcacctccgg cacctccagc accgcacccg ccttcgccgc
     3481 gcggtgcgcg gctatggcgc ggcagacctc
//
LOCUS       DAOPQX010000386         3501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_29123_length_3501_cov_3.125943, whole genome shotgun sequence.
ACCESSION   DAOPQX010000386 DAOPQX010000000
VERSION     DAOPQX010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29123_length_3501_cov_3.125943"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKK95_15485"
     CDS             <1..504
                     /locus_tag="PKK95_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19670.1"
                     /translation="VAAHLIYEGLVAEGLAVTKAVRDRYDGERRNPWNEVECGSHYAR
                     ALASWSLLTALAGYSYSAPEQRLTFAPRIAPEAFASFFTTGGGWGVFSQRADRGKTSA
                     ALDLRWGALELRRFGFGGTTAKGGRIAATVNGSPLSARLDEETIAFEQAVRLKAGDRL
                     EVEISNK"
     gene            complement(580..1677)
                     /locus_tag="PKK95_15490"
     CDS             complement(580..1677)
                     /locus_tag="PKK95_15490"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOC19671.1"
                     /translation="MARERRPFTVAEDIREARTLPAFVYSEPAVFELQRERVFASSWQ
                     LVGDLDQVKVPGQVRPTTFLEGILAEPLLLTRDAGDAVHCLSNVCTHRGTLVCEGEAV
                     LAQLRCRYHGRRFGLDGRFVSMPEFDGARDFPTRADDLAPVAFGTWGQLVFAGLDPAL
                     PFDDYIAPVRERCAWMPLDEAVLDPSRSRLYHVRANWALYFDNYLEGFHIPYVHASLA
                     GALDYAAYRTELFPWSSLQVGVARAGEDVFDLPSGSPDRGQAVAAYYFWLYPNLMLNV
                     YPWGISVNVVKPLAVDETRVTFLSYVWDPSRLDRGAGAGLDRVEREDESVVESVQKGV
                     RSRLYERGRYSPSREQGVHHFHRLLAQAMHG"
     gene            complement(1776..2759)
                     /locus_tag="PKK95_15495"
     CDS             complement(1776..2759)
                     /locus_tag="PKK95_15495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOC19672.1"
                     /translation="MEQRRLGPGLEVSAIGLGCMGMSDFYGPRDDGQSIETIHRALDL
                     GVTFLDTADAYGPFKNEELVGRAIRGRRDQVVIATKFGNERAADGTFIRVNGRPEYVR
                     RACEGSLSRLGVSHIDLYYQHRVDPTVPIEETVGAMAGLVREGKVRFLGLSEAAPATI
                     RRAHAVHPIAALQTEYSLWSREPEAEILPTVRELGIGFVAYSPLGRGFLGGRFRRYED
                     LPEDDWRRNNPRFTPENFARNQALLARIEAIAARKGATASQVALAWVLARGRDIVPIP
                     GTKRREYLEQNAAAVSIALSGEDFRELDEAFPRGAAAGERYPEAGMRAVNR"
     gene            2852..>3501
                     /locus_tag="PKK95_15500"
     CDS             2852..>3501
                     /locus_tag="PKK95_15500"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOC19673.1"
                     /translation="MRSYFCLLAALVLFPMTSSAQAPVSPLPPGSSCPEVERALTALM
                     RNDARLRDWAALGRYRQANAALPAPRPGESRVVFMGDSITDAWQQPRFGTFFEGRPYV
                     DRGISGQTTPQMLVRFRPDVLALQPKAVVILAGTNDIAGNTGPMTDEEIQGNLASMCQ
                     LASANGIKVVLASLTPVSEYHFNGPPGAPQTTLRPMARIRALNAWIERYARDNGHV"
BASE COUNT          516 a         1250 c         1222 g          513 t
ORIGIN      
        1 gtggcggcgc acctgatcta cgaggggctg gtggccgagg ggctggccgt caccaaggcc
       61 gtgcgagacc gctacgacgg cgagcgccgg aacccctgga acgaggtgga gtgcgggtcg
      121 cactacgcgc gggcgctcgc gagctggtcg cttctcaccg cgcttgccgg ctactcgtac
      181 tccgcgccgg agcagcgcct caccttcgcg ccgcggatcg cgcccgaggc gttcgcctcg
      241 ttcttcacga ccggcggcgg gtggggcgtg ttctcgcagc gcgccgatcg cggcaagacg
      301 tcggccgccc tcgacctgcg gtggggcgcc ctcgagctgc ggcggttcgg cttcggtggc
      361 actaccgcga agggcgggag gatcgccgcc acggtcaacg ggtctccact ctctgcccgc
      421 ctggacgagg agacgatcgc gttcgagcag gccgtgcggc tgaaggccgg cgatcggctg
      481 gaggtggaga tcagcaacaa ataggcggag ggcggagggc ggtgtaaccg acgaaacggc
      541 gaacgcagaa cggaacgtct ggaacgaaag acggcagggc tagccgtgca tcgcctgcgc
      601 cagcaggcgg tggaagtggt gcactccttg ctcgcgggag ggcgagtagc gcccgcgctc
      661 gtagaggcgc gagcggacac ccttctggac gctctcgacc acgctctcgt cctcgcgttc
      721 gacgcggtcg aggccggcgc ccgcgcctcg atccagccgc gacgggtccc agacgtacga
      781 cagaaaggtg acccgcgtct cgtccaccgc caggggtttc accacgttga ccgagatccc
      841 ccacgggtag acgttcaaca tcaggttggg atacagccag aagtagtacg cggccacggc
      901 ctggccccgg tccggcgaac ccgaaggcag gtcgaagacg tcctcgcccg cgcgcgccac
      961 ccccacttgc aggctcgacc acgggaacag ctccgtccgg taggcggcgt aatcgagggc
     1021 gccggccagc gacgcgtgaa cgtacggaat gtggaagccc tcgaggtagt tgtcgaagta
     1081 gagggcccag ttcgcgcgga cgtggtagag acgcgaccgg gacgggtcga gaaccgcctc
     1141 gtccaggggc atccaggcac atcgctcgcg caccggcgcg atgtagtcgt cgaagggcag
     1201 cgcgggatcg agcccggcga agaccagctg gccccacgtg ccgaacgcca ccggcgcgag
     1261 gtcgtcggcg cgggtcggga agtcgcgtgc gccgtcgaac tccggcatcg agacaaaccg
     1321 gccgtcgagc ccgaaccggc ggccgtggta ccggcatcgc agctgcgcga ggacggcctc
     1381 gccctcgcag accagggtgc cgcggtgggt gcagacgttc gagaggcagt gcacggcgtc
     1441 gcccgcgtcc cgcgtcagca gcagcggttc ggccaggatc ccttccagga acgtagtggg
     1501 ccgaacctgc ccgggcacct tcacctggtc gaggtcgccg accagctgcc agctcgaggc
     1561 gaacacgcgc tcgcgctgca gctcgaatac cgccggttcg gagtacacga acgccgggag
     1621 cgtccttgcc tcgcggatgt cctccgccac ggtgaacggc cgccgctcac gtgccattcc
     1681 gcccctccgt tcgctgtcgt tttccccttc cgccctccgc ctttcacgtt ctccggttcc
     1741 gctcccgcct ccgttccttc gccttctccg ttcccctacc tgttcaccgc gcgcatgccc
     1801 gcctccgggt accgctcgcc ggcggccgcg ccgcgcggga acgcttcgtc cagttcgcgg
     1861 aaatcctcgc ccgacagcgc gatcgagacc gcggcggcgt tctgctcgag gtattcgcgc
     1921 cgcttcgtcc cggggatcgg gacgatgtcg cggccgcggg cgaggaccca ggcgagggcg
     1981 acctgggagg cggtggcccc cttccgcgcg gcaattgcct cgatgcgtgc gagcagcgcc
     2041 tggttgcggg cgaagttctc gggagtgaag cgcgggttgt tccgccgcca gtcgtcctcg
     2101 ggcaggtcct cgtaccgccg gaagcggccg cctaggaagc cgcgcccgag cgggctgtag
     2161 gcgacgaacc cgatgcccag ctcgcggacg gtcggcagga tctcggcctc cggctcgcgc
     2221 gaccagagcg aatactccgt ctgcagcgcg gcaatcgggt gcacggcgtg ggcgcgccgg
     2281 atcgtcgcgg gcgccgcctc ggacaatccc aggaagcgga cctttccctc gcgcaccagc
     2341 ccggccatcg ccccgaccgt ctcctcgatg ggcaccgtcg ggtccacgcg atgctggtag
     2401 tagaggtcga tgtgggagac cccgaggcgg ctgagcgagc cctcgcaggc tcgccgtacg
     2461 tactcgggcc ggccgttgac ccggatgaag gtgccgtcgg cggcgcgctc gttgccgaac
     2521 ttggtggcga tgacgacctg gtcgcggcgc ccgcggatgg cgcgccccac cagttcctcg
     2581 ttcttgaagg ggccgtacgc gtcagcggtg tcgaggaagg tcacgccgag gtcgagcgcg
     2641 cgatgaatcg tctcgatcga ctgcccgtcg tcgcgcgggc cgtagaagtc gctcattccc
     2701 atgcagccga ggccgattgc cgacacttcc agccccgggc caagccgccg ctgttccatc
     2761 gcgtcctcca gtcctctcag catacgacac cggcgggggg cgtggctccc gaggctcgcc
     2821 gccggcggcc gcgtggacta cactaccgcg gatgcgaagc tacttttgcc tgcttgccgc
     2881 cctcgtgctg ttcccgatga cgtcttccgc gcaggccccc gtgtcccccc ttccgcccgg
     2941 ttcgtcctgc cccgaggtgg agcgggcgct caccgcgctg atgcgcaacg acgcgcggct
     3001 ccgcgactgg gccgccctcg ggcgctaccg ccaggcgaat gccgcgctcc ccgcgccgcg
     3061 gccgggtgag agccgtgtgg tgttcatggg cgactcgatc accgacgcgt ggcagcagcc
     3121 ccggttcggc accttcttcg aggggcggcc ctacgtggac cgcgggatca gcggccagac
     3181 caccccccag atgctggtgc gcttccggcc cgacgtgctg gccctccagc cgaaggccgt
     3241 ggtgatcctg gccgggacca atgacatcgc gggcaacacc gggccgatga ccgacgagga
     3301 gatccagggc aacctcgcct cgatgtgcca gctcgcgagc gccaacggca tcaaggtggt
     3361 gctggccagc ctcacgcccg tgagcgagta ccacttcaac ggtccccccg gcgcgcccca
     3421 gacgacgctg cggccgatgg cccgaatcag ggcgctcaac gcgtggatcg agcggtacgc
     3481 gcgcgacaac ggccacgtct a
//
LOCUS       DAOPQX010000387         3496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_29170_length_3496_cov_4.619297, whole genome shotgun sequence.
ACCESSION   DAOPQX010000387 DAOPQX010000000
VERSION     DAOPQX010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29170_length_3496_cov_4.619297"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKK95_15505"
     CDS             complement(<1..484)
                     /locus_tag="PKK95_15505"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19674.1"
                     /translation="MFVSVALPVPTLGLLTYKVPDGLEAPRPGTRVLVPLGARRLTGI
                     VVQAGEGRPGGLPQPGEGASIKTLIDVLDPEPLLPAMVVGLAEWVADYYACGPGEALG
                     AAMPPFAWVESELRARLTPAGRARVEASAGRRAATLRGAVLGLLGDGWTPLRSVAYRL
                     E"
     gene            complement(547..1299)
                     /locus_tag="PKK95_15510"
     CDS             complement(547..1299)
                     /locus_tag="PKK95_15510"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOC19675.1"
                     /translation="MDSRSALADHLEFLGELGVPGLSGDPAWRERPGEGAAQGAEAAA
                     GLQGEVAEAANGIAGDELEAVRLDLGENCERCKLHRLGRRQVVFGVGNPEARLMFVGE
                     APGRDEDEQGIPFVGRAGQLLTKMIEAMGYSREEVYIANVIKCRPPENRNPQPDEVFA
                     CEPFLFRQVEAIKPAVIVALGTFAAQALLKTQDSISRLRGRIFSYGGARLVPTFHPAY
                     LLRSPERKRDAWEDLKRAKALLDQDSDPASET"
     gene            complement(1302..2507)
                     /gene="coaBC"
                     /locus_tag="PKK95_15515"
     CDS             complement(1302..2507)
                     /gene="coaBC"
                     /locus_tag="PKK95_15515"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOC19676.1"
                     /translation="MPTIALGVTGGIGAYKAVEVARGLQKAGHEVVVVMTEAARHFVG
                     PLTFEAITRHPVITDQFAPGANADIAHIQLATDAALLLVAPATANCLGKMAHGIADDF
                     LSSLYLATRAPVVVAPAMNTFMWEHDAVRRNLEVLRERGVRVVEPGEGFLACGWMGKG
                     RLAEPDAIVAAVLDTLRKGASLAGRRFLVTAGPTFEDIDPVRYVGNRSSGRMGFAIAE
                     EAAARGAEVTVVAGPVSVAPPPGVALVRVRGAVEMRDAVMARAKGQDVIVMAAAVADY
                     RPERATEKIAKETQTLTLVLHRTPDILEGLGRERAEGRLPGTLLVGFAAETSEVVARA
                     REKRRRKQVDLVVANDVSRSDAGFDVDANAVVLVAEDGEQELGLAPKREIARGIVDRI
                     ERLLGTRKE"
     gene            complement(2519..3136)
                     /gene="gmk"
                     /locus_tag="PKK95_15520"
     CDS             complement(2519..3136)
                     /gene="gmk"
                     /locus_tag="PKK95_15520"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOC19677.1"
                     /translation="MSSERGRLFVVSAPSGTGKTTLARRLVQLIPNLGLSRSYTSRPI
                     REGERDGVDYHYVTPDRFLAMREAGEFLEWAEVFGRFYGTAVADTERRLAKGEDLVLV
                     IDVQGAAKVRARGLPFVGIFVLPPSFEALEARLRQRSRDPEEQIRCRLETARREIAAL
                     DDYDYVVINDEVERAVDGLRAIVLAERARREAMAGRVAGILRSFR"
     gene            complement(3126..>3496)
                     /locus_tag="PKK95_15525"
     CDS             complement(3126..>3496)
                     /locus_tag="PKK95_15525"
                     /inference="COORDINATES: protein motif:HMM:NF019940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HOC19678.1"
                     /translation="EQAATAAERGRSALETRLAERVRELAREVPGDPAAIAQEIVRVA
                     ARSDISEEVVRFRAHLAHWAALTAAEEPCGRKLDFLLQEMNREINTIGSKADGEGVPA
                     LVVAVKAELEKVREQVQNVE"
BASE COUNT          488 a         1323 c         1175 g          510 t
ORIGIN      
        1 gctccaaccg gtaggccacc gaccggagag gtgtccagcc gtcacccagc agtccgagga
       61 cggcaccgcg cagggtggcc gcccggcggc ctgcggatgc ttccacccgt gcgcggccgg
      121 caggcgtgag ccgggcgcgc agctcgctct cgacccatgc aaacggcggc atcgctgcgc
      181 ccagcgcctc ccccggaccg caggcgtagt agtcggccac ccactcggcc aggccgacca
      241 ccatcgccgg caggagcggc tccgggtcga gaacgtcgat gagcgtcttg atggaagcgc
      301 cttcgcccgg ctgcggcaag cctccaggcc tcccctcgcc agcctggacg acgatccccg
      361 tgagccgcct ggcgccgagc gggaccagca cgcgcgtgcc ggggcgcggc gcctcgaggc
      421 cgtctgggac cttgtaggtc agcaggccga gcgtcgggac cgggagggcg acggagacga
      481 acatggacgg gacagggaca ggaagacgta gaagccggaa tgctcccgca ttctaccttc
      541 cacgttctac gtttccgaag cagggtcgct gtcctgatcg agcagcgcct tggctcgctt
      601 caggtcttcc cacgcgtcgc gcttgcgctc ggggctccgc agcaggtagg cggggtggaa
      661 ggtgggcacc aggcgcgccc cgccgtagct gaagatccgt ccccgcagcc gggagatcga
      721 gtcctgcgtc ttgagcagcg cctgcgcggc gaaggtgccg agcgcgacga tcaccgcggg
      781 cttgatcgcc tccacttgcc ggaacaggaa cggctcgcac gcgaacacct cgtcgggctg
      841 cgggttccgg ttctcgggcg gccggcactt gatcacgttg gcgatgtaca cctcctcgcg
      901 gctgtagccc atcgcctcga tcatcttcgt cagcagctgc cccgcccgcc cgacgaaggg
      961 gatgccctgc tcgtcctcgt cacgtccggg cgcctcgccg acgaacatca gccgcgcctc
     1021 ggggttgccg acgccgaaga ccacctggcg gcggccgaga cgatgcagct tgcagcgctc
     1081 gcagttctcg cccaggtcca gtctgacggc ctcgagttcg tcgccagcga tgccgtttgc
     1141 cgcctccgcc acttcgccct gcagcccggc cgccgcctcg gctccctgcg ccgcgccttc
     1201 tcccgggcgt tcacgccacg cgggatcacc gctcaggccc ggcacaccca gctcgcccag
     1261 gaactcgaga tgatcggcca gcgcgcttcg cgaatccacc cttactcctt cctcgtgccg
     1321 agcaggcgct cgatccggtc cacgatgccg cgcgcgatct cccgcttcgg cgccaacccc
     1381 agctcctgct cgccgtcctc ggctaccagc accaccgcgt tggcgtccac gtcgaagccg
     1441 gcgtcggagc gggacacgtc gttggcgacg accaggtcca cctgcttccg gcgccgcttc
     1501 tctctcgccc gcgcgaccac ctcgctggtc tcggccgcaa acccgacgag cagggtcccg
     1561 ggcagccgcc cttccgcccg ctcgcgcccc agcccctcga ggatgtccgg ggtccggtgg
     1621 aggaccagcg tgagcgtctg ggtttccttg gcaatcttct ccgtcgcgcg ctcgggcctg
     1681 tagtcggcga ccgcggccgc catcacgatg acatcctgcc ccttggcccg ggccatgacg
     1741 gcgtcgcgca tctccaccgc gccgcgaacc cggacgagag cgacgccagg tggaggggcg
     1801 acgctgaccg ggccggcaac gacagtcacc tcggccccgc gggccgccgc ctcctcggcg
     1861 atggcgaacc ccatccgccc gctcgaccgg ttgccgacgt agcggaccgg gtcgatgtcc
     1921 tcgaaggtcg gacccgcggt gacgagaaac cggcgcccgg cgaggcttgc gccctttcga
     1981 agggtgtcga gcacggcggc cacgattgcg tcgggctcgg cgaggcggcc cttccccatc
     2041 cagccgcacg ccaggaagcc ctcgcccggc tccaccacgc ggacgccgcg ctcgcgcagc
     2101 acctcgaggt tgcggcgcac ggcgtcgtgc tcccacatga acgtgttcat ggccggcgcg
     2161 accacgaccg gcgcccgcgt ggccaggtac agcgacgaca ggaagtcgtc ggcgatcccg
     2221 tgcgccatct ttccgaggca gttggcggtt gccggcgcca ccagcagcag cgccgcgtcg
     2281 gtggccagct ggatgtgggc gatgtcggcg ttggcccccg gcgcgaactg atcggtgatg
     2341 accgggtggc gcgtgatcgc ctcgaaggtg agcgggccga cgaagtgccg ggctgcctcg
     2401 gtcatcacga cgacgacctc gtggcccgcc ttctgcaggc cgcgcgccac ctcgaccgcc
     2461 ttgtaggcgc cgatcccgcc ggtcactccc agggcgatgg taggcatctc tgtggccctc
     2521 agcgaaacga tcggaggatg cccgccaccc tccccgccat cgcctcgcgc ctggcacgct
     2581 cggcgagcac gatcgcgcgc aggccatcga cggcccgctc gacctcgtcg ttgatcacca
     2641 cgtagtcgta atcgtcgagg gccgcgatct cgcgtcgagc cgtttcgagg cggcagcgga
     2701 tctgttcctc ggggtcgcgg ctgcgctggc ggaggcgcgc ttcgagcgcc tcgaaagaag
     2761 gcggcagcac gaagatcccg acaaacggga ggccgcgcgc ccggaccttc gcggcacctt
     2821 gcacgtcgat gacgaggacg aggtcttcac ccttcgccag ccgcctttcg gtgtcggcga
     2881 cggcggtgcc gtagaaccgc ccgaacacct cggcccactc gaggaattcg cccgcctcgc
     2941 gcatggcgag gaagcggtcg ggcgtgacgt agtggtagtc gacgccgtcg cgctcgccct
     3001 cgcggatcgg ccgcgaggtg tacgaccgcg agaggccgag gttgggtatc agctgcacga
     3061 gccgccgcgc cagcgtcgtc ttcccggtgc cggagggggc cgacacgacg aacagccggc
     3121 cgcgctcact cgacattctg cacctgctcg cggaccttct cgagctcggc cttcacggcg
     3181 accaccaggg ccggcactcc ctcgccgtcg gccttgctgc caatcgtgtt gatttcgcgg
     3241 ttcatctcct gcaggaggaa gtcgagcttg cggccgcaag gctcctcggc cgcggtcagc
     3301 gccgcccagt gggccaggtg cgcgcggaac cgcaccacct cttcgctgat gtccgagcgg
     3361 gccgccaccc gcacgatctc ctgggcaatc gcggccgggt cgccgggcac ctcgcgggcc
     3421 agctcgcgca cccgctcggc gagccgcgtc tcgagcgccg agcggccgcg ctcggcggcg
     3481 gtggccgcct gctcca
//
LOCUS       DAOPQX010000388         3484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_29317_length_3484_cov_4.001167, whole genome shotgun sequence.
ACCESSION   DAOPQX010000388 DAOPQX010000000
VERSION     DAOPQX010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29317_length_3484_cov_4.001167"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1416
                     /locus_tag="PKK95_15530"
     CDS             124..1416
                     /locus_tag="PKK95_15530"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOC19679.1"
                     /translation="MRWLRCWYVLALAVLCLLPPRGASPQDDLLARARRLHAEVPLVD
                     GHNDNPWQVRERAGGDPAKLDISRPQPSLQTDLARLRAGGVGAQFWSVYVPPSYAGDR
                     AVSATLEQIDLVHRMIERYPNDLELALTAGDIERIHRRGKVASLIGVEGGHSINGSLG
                     VLRMLYRLGARYMTLTHGQNVPWADSATDQRVHGGLTPFGREVVREMNRLGMLVDLSH
                     VSPETMEDVLETTAAPVVFSHSSARALVDVPRNVPDAVLKRLPANGGVVMVSFVPDFT
                     SREMAVWAEADEREQARLSALFPKAPERVRQERAAWRQAHPAPGATLEQVADHVDHVR
                     RVAGIDHVGLGSDFDGITTTPRGLPDVSAFPALTAELLRRGYSEEDVRKVIGLNVLRV
                     MRSVERIGSAGLRAGGTITAPAPPPPDPQPPRIRPPAS"
     gene            complement(1352..2158)
                     /locus_tag="PKK95_15535"
     CDS             complement(1352..2158)
                     /locus_tag="PKK95_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19680.1"
                     /translation="MTPGKRSLWLRPVLAGAAALAARHPVSRAGFSFTLQTLARSGKH
                     RLYLAGYLALGVALAAVVASSAAATSTAAHPNLAPGLMALQMTLVFALVAGLRSVVEI
                     PAELRSNWVFRTCWNGDIRRYVMGVRRAFVLGIVTPLLALLMPLHVFWWGWGLALRHA
                     AIDLLAALVLLEAALVGFRKLPFTCSYLPKGTFKFLWPAYLVAFFASTYLLATIERPA
                     LAGDEALLRLAAGLVLAVAALRLYARWRLVKKPEVVFEEAADPAAVALGL"
     gene            complement(2155..2583)
                     /locus_tag="PKK95_15540"
     CDS             complement(2155..2583)
                     /locus_tag="PKK95_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC19681.1"
                     /translation="MLEARGLTKFYSGIAAVKDVSFQVRAGEILGYLGPNGSGKTTTV
                     NMIIGLVEPSRGEVLFEGRPIAADPAGFRRRVGYVPEEPNLYPFLSGREYRAHGTSGC
                     CPRCGTRGGRLLSRSAPPGRWRSCCTSSGTHARPGACGNR"
     gene            complement(2635..>3484)
                     /locus_tag="PKK95_15545"
     CDS             complement(2635..>3484)
                     /locus_tag="PKK95_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HOC19682.1"
                     /translation="DFSITFDRLAGTMRSYSVRGRRLIERGPVPDFWRAMTDNDLGAW
                     KSVGFAARKNPALDTTIWREAGPAWRVKDVEVARLDDGRARVRVSGVLPVNQAPYDVT
                     YTIAGDGEVVVDASYTPGAGPVAMMPRFGMQLVLAAGLETMTWYGRGPHETYWDRAFE
                     PVGVFTGTVAGQWVEYSRPQENGNKVDVRWVTLTGPDGIGLRAQGDPVLEVSASHATA
                     RDLEKADYTFQVPRRAQVFLNLDHRQMGVGGVDSWSQKAWPLEPYRLPGDRPYRYRFT
                     LTPVLK"
BASE COUNT          543 a         1218 c         1239 g          484 t
ORIGIN      
        1 aggtgctggt gccgaaggtg ctggaggtgc tggtgccgaa ggtgctggag gtgcttgtgc
       61 cgaaggtgct gatggtgctg gtgtgaacgt gcaggtgctg ataccgtggg tgcgaggttt
      121 cccatgagat ggctgcggtg ctggtacgtg ctcgcgctcg ccgtgctgtg tctccttcct
      181 cctcgcggcg cctccccgca ggacgacctg cttgcgcggg cgcggcggct gcatgccgag
      241 gttccgctgg tggacggcca caacgacaac ccgtggcagg tgcgcgagcg ggccggcggc
      301 gacccggcga agctcgacat ctcgaggccg cagccgtccc tgcaaaccga cctggcgcgg
      361 ctgcgcgcgg gtggcgtggg agcgcagttc tggtcggtct acgtgcctcc ctcgtacgca
      421 ggcgaccgtg ccgtctcggc gacccttgaa caaatcgacc tcgtccaccg gatgatcgag
      481 cgctatccca acgacctcga gctggcgctc accgccggcg acatcgagcg gattcaccgg
      541 cggggcaagg tcgcttcgct catcggcgtc gagggcgggc actcgatcaa cgggtcgctc
      601 ggcgtgctgc ggatgctgta ccggctgggc gcccgctaca tgaccctgac gcacggacag
      661 aacgtgccct gggccgactc ggcaaccgac cagcgggtgc acggcggcct gacgccgttc
      721 gggcgcgagg tcgtgcgcga gatgaaccgc cttggaatgc tggtggacct gagccacgtg
      781 tcccccgaga cgatggagga cgtgctcgag acgacggcgg cgccggtcgt cttctcgcac
      841 tcgagcgcgc gggcgctggt ggacgtgccg cgcaacgttc ccgacgcggt cctcaagcgg
      901 ctgccggcga acgggggcgt ggtgatggtc agcttcgttc ccgacttcac gtcgcgggag
      961 atggcggtct gggcggaggc cgacgagcgc gagcaggccc ggctcagcgc cctcttcccg
     1021 aaggcgccgg agcgcgtcag gcaggagcgt gccgcgtggc ggcaggcgca tcctgccccc
     1081 ggcgccacgc tcgagcaggt ggccgaccac gtcgaccacg tgcggcgcgt cgccgggatc
     1141 gatcacgtcg gtctcggcag cgacttcgac ggcatcacga cgacgccgcg aggcctcccc
     1201 gacgtcagcg cgttccccgc cctgaccgcc gagctgctgc gccgcggcta cagcgaggag
     1261 gacgtcagga aggtgattgg gctcaacgtg ctcagggtga tgaggagtgt tgaacggatc
     1321 ggtagcgccg gcctccgtgc cggcggcacc atcacagccc cagcgccacc gccgccggat
     1381 ccgcagcctc ctcgaatacg acctccggct tcttgacgag gcgccagcgc gcgtagagcc
     1441 gaagagcggc caccgccagc acgagtcccg ccgcgaggcg cagcagcgct tcgtcgccgg
     1501 ccagcgccgg ccgttcgatg gtcgcgagca ggtacgtgct cgcgaagaac gccacgaggt
     1561 acgcgggcca caggaacttg aaggtcccct tcgggaggta cgaacaggtg aagggcagct
     1621 tgcggaagcc gacgagcgcc gcctcgagca ggacgagggc ggccagcagg tcgatcgccg
     1681 cgtggcgcag cgcaagaccc cacccccacc agaacacgtg aaggggcatc agcagcgcaa
     1741 gcaggggagt gacgatgccg aggacgaacg ctcgccggac acccattacg tagcgccgga
     1801 tgtcgccgtt ccagcacgtg cggaacaccc agttcgatcg cagctcggca gggatctcga
     1861 ccacagagcg gagccctgcg accagggcga agacgagcgt catctgcagc gccatcaggc
     1921 ccggcgcgag gttcgggtgt gccgccgtgc tcgtggcggc ggcgctcgac gccacgacag
     1981 cggcaagggc cacgccgagc gccaggtacc cggcgaggta caggcggtgc ttcccgctgc
     2041 gcgccagcgt ctggagggtg aacgagaacc cggcgcgcga caccgggtgg cgtgcggcga
     2101 gcgcggcggc acccgccagc acgggacgca gccacaggct gcgctttccc ggcgtcatcg
     2161 attcccgcag gcgcccggcc tggcgtgcgt gcccgacgac gtacagcagg accgccagcg
     2221 cccaggcggt gccgaacgcg acaggagcct tccgcccctc gtgccgcagc gcgggcagca
     2281 cccgctcgta ccgtgcgcgc ggtactcgcg gccggagagg aacgggtaga ggttcggctc
     2341 ttcggggaca tagccgaccc ggcggcggaa gccagccggg tccgcggcaa tcgggcggcc
     2401 ctcgaacagg acctcgccgc ggctcggctc caccaggccg atgatcatgt tgaccgtggt
     2461 ggtctttcca gagccgttgg ggccgagata gccgaggatc tcgccggccc gcacctggaa
     2521 ggagacgtcc ttcaccgccg cgatgcccga gtagaacttg gtcaggcccc gcgcttcgag
     2581 cattaacccc tccagcctat tggacaccgg cgggaagagg acggattcac gacgtcattt
     2641 cagcacgggc gtcagcgtga agcgatagcg gtacggacgg tcccccggga ggcgatacgg
     2701 ctcgagcggc caggccttct ggctccagct gtcgacgccc cccacgccca tctggcggtg
     2761 atccaggttg aggaacacct gcgcccggcg cggcacctgg aacgtgtagt cggccttctc
     2821 gaggtcgcgc gccgtcgcgt gcgaggccga cacctcgagc acggggtcgc cctgggccct
     2881 caggccgatg ccgtccgggc cggtgagcgt cacccaccgg acgtccacct tgttgccgtt
     2941 ctcctgcggc cgcgagtatt cgacccactg accggcgacc gtccctgtga agacgccgac
     3001 cggctcgaaa gcgcgatccc agtacgtctc gtggggcccg cggccgtacc aggtcatcgt
     3061 ctcgaggccg gcggccagca ccagctgcat gccgaaacgg ggcatcatcg cgaccggacc
     3121 cgcgcccggc gtgtaggacg cgtccaccac cacctcgccg tcgcccgcga tcgtgtaggt
     3181 gacgtcgtac ggcgcctggt tgacgggcag gacgcccgac acccgcacgc gcgcccgccc
     3241 gtcgtccagc cgcgcaacct cgacgtcctt cacccgccag gcgggcccag cctcgcgcca
     3301 gatcgtcgtg tcgagcgcgg ggttcttccg ggcggcgaac ccgaccgact tccaggcgcc
     3361 caggtcgttg tcggtcatcg cccgccagaa gtcgggcacc gggccccgct cgatcaaccg
     3421 ccttccgcgc acgctgtacg agcgcatggt tccggcgagg cggtcgaagg taatcgagaa
     3481 gtcc
//
LOCUS       DAOPQX010000389         3479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_29356_length_3479_cov_4.932827, whole genome shotgun sequence.
ACCESSION   DAOPQX010000389 DAOPQX010000000
VERSION     DAOPQX010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29356_length_3479_cov_4.932827"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PKK95_15550"
     CDS             <1..224
                     /locus_tag="PKK95_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19683.1"
                     /translation="GVLWGLAVWAGSYLGWLPAAGIRPSSEKQPPRREALMIAAHVVF
                     GAATGLAYQALARRGRGSPAPGDMIGAGI"
     gene            227..1279
                     /locus_tag="PKK95_15555"
     CDS             227..1279
                     /locus_tag="PKK95_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOC19684.1"
                     /translation="MRIAAVRHAFPPHYYDQAELIAAFRRAWGRRHYNLDRLEQLHRN
                     VLVGGRHLALPLEEYDRLETWGQANDAWIRVGEEVGAEAVRQALAAAGLDAGDVDVIV
                     FVSVTGISTPSLDARLVNRLNLPAHVRRVPIFGLGCVAGAAGLSRAADLLRGSGGQVA
                     VLLSVELCSLTLQREDLSVPNLIATGLFGDGAAAAVVAADDAPQCHGPRIVATRSVFY
                     PGSEHVMGWSISESGFRVVLSPDVPAVVLGKLGGDVDSFLRDQGLTRGDIRAWISHPG
                     GPKVLEAMQQALGLDAAALEASWRSLREAGNLSSASILLVLEDVMRDAAPPPGGHGLM
                     IAMGPGFCSELVLLRW"
     gene            1303..2061
                     /locus_tag="PKK95_15560"
     CDS             1303..2061
                     /locus_tag="PKK95_15560"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOC19685.1"
                     /translation="MDTRLAYSLLVAAVAAERGLELLISRRNGKRARARGAIEAGRSH
                     YPAMVALHAAFLAACPLEVWLLGRPWLPALGIPMLGLVLAAQGIRYWVVHTLQGRWTT
                     NVLYVPGDPPITAGPYRWARHPNYVAVTAEMAALPLVHTAWLTAVVFSAANVLLLAVR
                     IKAEERLLEEADILAGVTEVARSHLGFTGTLSPDLPLSAALALDSLRQLTLVVEIESR
                     FRIALDDDAESLRTVGDLVAAIRRKREGRAPDAR"
     gene            2033..>3479
                     /locus_tag="PKK95_15565"
     CDS             2033..>3479
                     /locus_tag="PKK95_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOC19686.1"
                     /translation="MKDAPPTLVDLLRRAAASDTSGIRVLDRKGEQAAWLSWAGVWHR
                     ALRVAGGLAARGVARGDRVALVFPTGPPFIESFFGTVAAGAVPVPLYPPVRLGRLDEY
                     HESAARMIGSVEAKLVLADRRVRRLLGETARRARPPLGCLVPDDLPGGVGEAASPDPG
                     DIALIQFSSGTTRDPKPVALSHRAVVGQVTALNGFWPDEGGVVHSGVSWLPLYHDMGL
                     IGCLFTALERPGTITLIPPEAFVAAPAVWLRAISTWRATISPAPTFAYSLAAHRIRDE
                     DLEGVDLSSWRVALCGAETVVADVLREFARRFARWGFDERALTPVYGLSEASLAVTFS
                     QLGGGARATRFDRDGLESEGIARPHEGGREIVSVGRPIPGVELRIADADGRPVPEGRV
                     GEVQCRGPSTMEGYFGRPEASAAAWCDGWLRTGDLGFVSGGELYLVGRLRDILLLRGR
                     NHDPEEVERAVEGVAGVRDGCTVAVSWLPEGA"
BASE COUNT          413 a         1185 c         1329 g          552 t
ORIGIN      
        1 gaggggtgct ctgggggctg gccgtctggg ccggaagcta cctcggttgg ctgccggccg
       61 ctggcatccg cccctcgtct gagaagcagc cgccgcgccg ggaggcgctg atgatcgcgg
      121 cacacgtggt gttcggcgcc gcgacgggac ttgcctacca ggcgctcgcc aggcgcggcc
      181 gcggcagtcc cgcgccgggt gatatgatcg gcgccgggat ctgaccgtga gaatcgccgc
      241 cgtgcggcac gcgttcccgc cgcactacta cgatcaggcg gaactcattg ccgccttcag
      301 gcgtgcctgg gggcggcgcc actacaacct cgatcgcctc gagcagctcc accgcaacgt
      361 ccttgtcggc gggcgccacc tcgcgctgcc gctcgaggag tacgaccgcc tcgagacctg
      421 ggggcaggcc aacgacgcct ggattcgggt gggggaggag gtcggcgccg aggcggtgcg
      481 ccaggcgctc gcggcggcgg ggctcgatgc gggcgacgtg gacgtcatcg tcttcgtgtc
      541 ggtcaccggc atttccacgc cgtcgctcga tgcccgcctc gtcaaccggc tgaaccttcc
      601 cgcgcacgtg cggcgggtgc cgatcttcgg cctcgggtgt gtcgccggcg cggccggcct
      661 ttcgagggct gcggacctgc tgcgaggcag cgggggccag gtggccgtcc tcctgtcggt
      721 cgagctgtgt tcgcttaccc tgcagagaga agacctctcc gtcccgaatc tcatcgccac
      781 cggcctcttc ggcgacggag cggcggcggc ggtcgtagcc gccgatgacg cgccgcagtg
      841 ccacgggccc cgcatcgtgg cgacacggtc ggtcttctac ccaggctccg agcacgtgat
      901 ggggtggtcc atctcggagt cgggcttccg cgtcgtcctc tcgcccgacg tgccggcggt
      961 ggtgctgggg aagctcggag gtgatgtcga ctccttcctt cgcgaccagg gactgacgcg
     1021 cggcgacatc cgggcgtgga tctcgcaccc cggcgggccc aaggtgctcg aggcgatgca
     1081 gcaggcgctc ggcctcgacg ccgccgccct cgaggcgagc tggcgaagcc tccgcgaggc
     1141 gggaaacttg tcgtcggcgt cgatcctcct ggtgctcgag gacgtgatgc gcgacgccgc
     1201 cccgccgccc ggcgggcacg ggctgatgat cgcgatggga ccgggtttct gcagcgagct
     1261 ggtcctgctg cggtggtgac gccgccggcc tggacacgat cgatggacac gcgcctcgcc
     1321 tacagcctgc tggtggccgc cgtcgcggcg gagcgcgggc tcgagctgct gatctcccgc
     1381 cggaacggga agcgtgcgcg cgcccgcggc gcgatcgagg ccgggcgctc gcactacccg
     1441 gcgatggttg ccctgcatgc tgccttcctc gcggcgtgtc cgctcgaggt gtggctgctc
     1501 ggacgccctt ggctgcctgc gctgggcatc ccgatgctcg gcctggtgct cgcggcgcag
     1561 ggaatacgct actgggtggt ccacacgctg cagggacgtt ggaccaccaa cgtgctgtac
     1621 gtgcctggtg atcccccgat caccgcgggc ccctaccgct gggcccgcca tcccaactac
     1681 gtcgcggtga ccgcggagat ggcggccctt ccgctggtgc acaccgcctg gctcaccgcg
     1741 gtggtcttca gcgcggcgaa cgtgctgctg ctcgccgtcc ggatcaaggc cgaggagcgc
     1801 cttctcgagg aggccgacat cctggcgggc gtgacggagg tggcgcgcag ccacctcggc
     1861 ttcaccggta cgctctcgcc cgacctgccg ctctcggccg cgctcgcgct cgattcgctg
     1921 cgtcagctca cgctggtggt ggagatcgag agccggttca ggattgccct ggacgacgac
     1981 gcggaaagcc tgagaaccgt gggcgacctg gtcgccgcaa tccggaggaa gcgtgaagga
     2041 cgcgcccccg acgctcgttg acctgctgcg ccgggcggcg gcaagcgaca cgagcggcat
     2101 tcgcgtgctg gatcggaagg gggagcaggc ggcgtggctg tcgtgggccg gcgtctggca
     2161 ccgcgccttg cgggtggccg gcgggctcgc ggcccgaggc gtggcgcggg gcgaccgcgt
     2221 tgcgctggtt ttccccaccg ggcctccctt catcgaatcg ttcttcggca ccgtcgcggc
     2281 cggcgccgtc ccggtgccgc tctatccgcc cgtccgcctt ggccgtctcg acgagtacca
     2341 cgagtcggcg gcccggatga tcggctcggt cgaggcgaag ctcgtgcttg ccgatcgcag
     2401 ggtcaggagg ctgctgggcg agaccgccag gcgcgcccgg ccgccgctcg ggtgtctcgt
     2461 gcctgacgac ctgccgggcg gcgtgggaga ggccgcctca cccgatcccg gcgacatcgc
     2521 cctgatccag ttttcctcgg ggacgacgcg cgatccgaag ccggtggcgc tgtcgcaccg
     2581 cgcggtggtc gggcaggtga ccgcgctgaa cggcttctgg cccgacgagg gcggcgtcgt
     2641 gcactcgggc gtgagctggc tgccgctcta ccacgacatg gggctgatcg gctgcctgtt
     2701 caccgcgctc gagcggccgg gcaccatcac cttgatcccg cccgaggcgt tcgtcgcggc
     2761 gcctgcagtc tggctgcgcg ccatctccac ctggcgggca acgatctcgc cggcgccgac
     2821 gtttgcttac agccttgcgg cgcaccggat ccgggacgag gacctcgagg gggtggacct
     2881 gtcgtcgtgg cgtgtggcgc tgtgcggtgc cgagacggtg gtggccgacg tcctgcgaga
     2941 gttcgcgcgg cgtttcgcgc ggtggggatt cgacgagcgg gcgctgacac cggtctacgg
     3001 cctctcggaa gcgtcgctgg ccgtcacctt cagtcagctg ggtggcggcg cgagagcgac
     3061 acggttcgac cgcgacgggc tcgagtcaga ggggatcgcg cggccgcacg agggagggcg
     3121 ggagatcgtg tcggtcggcc ggccgattcc cggcgtcgag ctgcggatcg ccgacgcgga
     3181 cgggcgtccc gtgcccgagg ggcgcgtcgg cgaggtgcag tgccgcggtc catcgacgat
     3241 ggaggggtac ttcgggaggc cggaggcgag cgcggcggcg tggtgcgacg gctggctgcg
     3301 gacgggagac cttggcttcg tatcgggcgg cgagctgtac ctcgttgggc ggcttcgcga
     3361 catcctgctc ctgcgcggcc gcaaccacga cccggaggag gtcgagcgcg cggtggaggg
     3421 tgtggcgggg gtgcgggacg gctgcacggt ggcggtcagc tggctgccgg aaggggccg
//
LOCUS       DAOPQX010000390         3472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_29434_length_3472_cov_5.117062, whole genome shotgun sequence.
ACCESSION   DAOPQX010000390 DAOPQX010000000
VERSION     DAOPQX010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29434_length_3472_cov_5.117062"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /gene="nadA"
                     /locus_tag="PKK95_15570"
     CDS             <1..701
                     /gene="nadA"
                     /locus_tag="PKK95_15570"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOC19687.1"
                     /translation="VLLPDLAAGCSLSDQCPAPEFERFVRAHPGHTVITYINSSAAVK
                     ALSDVICTSSNAERIIRQIPAGRPIIFAPDQQLGRYLIARTGRDMVLWPGACSVHVAF
                     SERRLLRLKAEHPEALVLAHPECETPVLRLADHIGSTSSILKYALESPAQAFIVVTEA
                     GIIHQMKKAAPGKTFIPAPPDSGCSCNECPFMRLNTLEKVYLCMRDCRPEITMDETLR
                     QRCLVPLRRMLEMS"
     gene            858..1739
                     /gene="nadC"
                     /locus_tag="PKK95_15575"
     CDS             858..1739
                     /gene="nadC"
                     /locus_tag="PKK95_15575"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOC19688.1"
                     /translation="MSRERFRRGSVAEFDEAFLRSEVRRFLEEDVGPGDATTRAVIPP
                     GARASGWIVARQPCVVAGLDLARAVFEQLDPSTCFEAAVSDGEQVPAGARLARLEGLA
                     AVLLTGERLALNLLQRLSGVATVTRRYVEAIAGTGASVSDTRKTTPGLRLFEKRAVSL
                     GGGRNHRLGLHDGVLIKDNHVAAAGGVGAAVRAARAVSSGLPIQVEVDSLEQLEEALD
                     AGADAVLLDNMTPGQTAAAVARARAHPRGADCWIESSGGITLQNVRAYAEAGVDTISV
                     GALTHSAPAVDIALDFE"
     gene            1777..3339
                     /gene="nadB"
                     /locus_tag="PKK95_15580"
     CDS             1777..3339
                     /gene="nadB"
                     /locus_tag="PKK95_15580"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOC19689.1"
                     /translation="MPHTPEIDVLVIGSGLAGAAAALAAARQGARVVMITKTDRPEEG
                     NTWYAQGGIIYHGANDSPERLVSDILAAGAGLSDPAAAALLAREGPRLVKEVLIEQAG
                     VPFDINGEGGLDLTSEAAHSVARIAHYQDATGRAIESSMLDRLGREPGATVLCRRTAV
                     DLLTLSHHSRDPLDVYAPPTCVGAYVFDQETRRVETMLAKETILATGGLGRVYLHTTN
                     PAGACGDGVAMAYRAGARCLNMQFVQFHPTTLYHPGERFLISESLRGEGARLVDPAGR
                     EFMNAYHPDGSLAPRDIVARGIHRMMHETGAECAFLDITHRPAAWVRKRFPQIHARCL
                     EAGIDITRERIPVVPAAHYSCGGIAVDDRGRTSIRRLRAAGEVSCTGLHGANRLASTS
                     LLECLVWGTRAGDDAGQAAVSRSSEYAVPEVAPWQYETEPVDPALMAQDWLTIRQTMW
                     NYVGLVRSRRRLERAHEILRELHMEIGRFYVRGELSDELIGLRNGVQTSLAVLLAAME
                     ARESRGCHYRVD"
BASE COUNT          502 a         1230 c         1237 g          503 t
ORIGIN      
        1 tggtcctgct gcccgacctc gccgcgggct gctcgctctc ggaccagtgc cccgcgcccg
       61 agttcgagcg cttcgtgcgg gcgcatcccg ggcacacggt catcacctac atcaactcga
      121 gcgccgcggt gaaggcgctg agcgacgtca tctgcacctc gagcaacgcg gagcggatca
      181 tccggcaaat ccccgccggc cgcccgatca tcttcgcgcc cgaccagcag ctcgggcgct
      241 acctgattgc gaggaccggg cgcgacatgg tgctctggcc gggcgcctgc tcggttcacg
      301 tggcgttctc ggagcggcgg ctgctgcggc tgaaggccga gcaccccgag gcgctggtgc
      361 tcgcgcaccc cgagtgcgaa accccggtgc tccgcctggc cgaccacatc ggctccacct
      421 cgtcgatcct caagtacgcc ctggagagcc ccgcgcaggc gttcatcgtg gtgaccgagg
      481 cggggatcat ccaccagatg aagaaggcgg cgcccggcaa gacgttcatc cccgccccgc
      541 ccgacagcgg ctgctcgtgc aacgagtgcc ccttcatgcg gctcaacacg ctcgagaagg
      601 tgtacctctg catgcgcgat tgccgccccg agatcacgat ggacgagacg ctgcgccagc
      661 gctgcctcgt cccgctgcgg cggatgctcg agatgagctg agcgtccacg ttcaccacag
      721 aggcacagag gcacggagac gcacgcagag gtttcgacgt cccggcaagg ggtcgggagt
      781 aattagaagt cgtgccgagt ttccgacttc taattactcc cgacccctac gcttctgtgg
      841 ttcaattcct tcagcgtatg tcgagagagc ggttccggcg cggttcagtg gcggagttcg
      901 acgaagcctt cttgcgcagc gaggtccggc gcttcctcga ggaggacgtg ggcccgggtg
      961 acgcgaccac ccgcgcggtg atcccgccgg gcgcccgcgc gtcaggctgg atcgtcgcgc
     1021 ggcagccctg cgtggtggcc gggctcgatc tggcgcgcgc cgtcttcgag cagctggacc
     1081 cttcgacgtg cttcgaggct gccgtctccg atggcgaaca ggtgccggcg ggcgcccggc
     1141 ttgcccggct cgagggcctc gcggccgtcc ttctgaccgg tgagcggctg gccctcaacc
     1201 tgctgcagcg cctgtcgggc gtcgccaccg tgacccgccg ctacgtcgag gcaattgcgg
     1261 gcacgggcgc gtcggtctcc gacacgcgca agaccacgcc cggcctccgg ctgttcgaga
     1321 agcgggcggt atcgctgggc gggggccgca accatcgcct cggcctgcac gacggcgtgc
     1381 tgatcaagga caatcacgtc gcggccgccg gcggtgtcgg ggccgcggtc cgggcggcgc
     1441 gcgcggtctc gtccggcctc ccgatccagg tggaggtgga ttcgctcgag cagctggagg
     1501 aggcgctcga cgcgggcgcc gacgcggtgc tgctcgacaa catgaccccg ggccagaccg
     1561 ccgcggccgt ggcacgcgcc cgcgcgcatc cccgcggcgc cgactgctgg atcgagtcct
     1621 ccggtggaat taccttgcag aacgtgcggg cgtacgccga ggcgggggtc gacacgatct
     1681 cggtcggggc gctgacgcac tcggcgccag ccgtggacat cgcgctggat tttgagtagg
     1741 gatcagggga acgactggac gcggcgcttc agcgagatgc ctcacacacc cgagattgac
     1801 gtactggtga tcggcagcgg cctggccggt gccgccgccg ccctcgccgc cgcccgccag
     1861 ggcgcgcggg tggtgatgat caccaagacc gaccgccccg aggaaggcaa cacctggtac
     1921 gcccagggcg gcatcatcta ccacggcgcg aacgactccc ccgagcggct cgtctccgac
     1981 atcctcgcgg ccggcgccgg cctgtccgac ccggccgccg ccgccctgct ggcgcgcgag
     2041 gggccgcggc tcgtgaagga ggtcctgatc gagcaggcgg gcgtcccgtt cgacatcaac
     2101 ggggagggcg ggctcgacct cacctcggag gccgcccact cggttgcccg catcgcccac
     2161 taccaggacg ccaccgggcg ggccatcgag tcgtcgatgc tggatcgcct cgggcgcgag
     2221 ccgggggcga cggtgctgtg ccgccgcacc gccgtggacc tgctgacgct ctcgcaccac
     2281 tcgcgcgacc cgctcgacgt ctacgcgcct cccacctgcg tcggggcgta cgtcttcgac
     2341 caggagacgc gccgcgtcga gacgatgctc gccaaggaga cgattctggc caccggcggc
     2401 cttggccgcg tctacctgca caccaccaac ccggcggggg cgtgcggcga cggcgtggcc
     2461 atggcgtacc gcgccggggc ccgctgcctg aacatgcagt tcgtgcagtt ccacccgacg
     2521 accctgtatc acccggggga gcgcttcctg atttccgagt cgctgcgggg cgagggtgcg
     2581 aggctggtgg acccggccgg gcgcgagttc atgaacgcct accatcccga cggatcgctc
     2641 gcgccccgcg acatcgtggc gcgcggcatc caccggatga tgcacgagac cggtgccgag
     2701 tgcgccttcc tcgacatcac ccaccggccg gccgcctggg tgcgcaagcg cttcccgcag
     2761 atccacgccc gctgcctcga ggcgggaatc gacatcacga gagagcggat cccggtggtg
     2821 ccggcggccc actacagctg cggcggcatc gccgtggacg accgcgggcg caccagcatc
     2881 cggaggctcc gcgcggccgg cgaggtgtcg tgcaccgggc tccacggcgc caaccgcctg
     2941 gccagcacct cgctgctcga gtgcctcgtg tggggcacgc gggccggcga cgacgccggc
     3001 caggccgcgg tcagccggtc atcggagtac gccgtcccgg aggtggcccc ctggcagtac
     3061 gagaccgagc cggtcgatcc cgccctgatg gcgcaggact ggctgacgat ccgccagacg
     3121 atgtggaact acgtggggct ggtgcgcagc cggcggcgcc tcgagcgcgc gcacgagatc
     3181 ctccgcgagc tgcacatgga gatcgggcgc ttctacgtgc gcggcgagct gagcgacgag
     3241 ctgatcggcc tgcgcaacgg cgtgcagacg tcgctcgcgg tgctgctggc cgcgatggag
     3301 gcccgcgaga gccgcggctg ccactaccgg gtggattgag cgtctgtcac gcggcaggcc
     3361 gcagagaagc aaagggatca gggatcagat cgtgccggag gtgcagaggg tgcaggcggt
     3421 gccgaaggtg ctggtgctga tggtgccgaa ggtgctggtg ctgatggtgc cg
//
LOCUS       DAOPQX010000391         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_29763_length_3444_cov_4.819121, whole genome shotgun sequence.
ACCESSION   DAOPQX010000391 DAOPQX010000000
VERSION     DAOPQX010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29763_length_3444_cov_4.819121"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..985)
                     /locus_tag="PKK95_15585"
     CDS             complement(215..985)
                     /locus_tag="PKK95_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOC19690.1"
                     /translation="MTIRTIVVDDEPLGRERIVSLLAGQADVQVVAECGDGRSAIEAV
                     RREAPHLVFLDVQMPERDGFAVMEAVGGERLPLVVFVTAYEAYALRAFEVRALDYLLK
                     PFDQARFQQTLQRVRTELTHQESDALARRVLALATEFRPERPPATDRLVVKSAGRVSF
                     LRTREIDWIEASGNYLKLHVGAESYLVRQTMSLMEEQLDPSTFVRIHRSQIVNIDRIK
                     EMHPLFNGEYEVVLRTGARLTLSRGYRDKLQSRLGGAI"
     gene            complement(982..2106)
                     /locus_tag="PKK95_15590"
     CDS             complement(982..2106)
                     /locus_tag="PKK95_15590"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOC19691.1"
                     /translation="MRSAAPRVLLVLAVASLLGLFSALLAYSFRLASSEPVSWWHTFP
                     LNLAYWWAWAALTPFVFWMARRFRFGRRAIWRPLLAHLPAAALTSLVHVMTLSLVRGA
                     LTGRLSWARWLSDVRSSYWMSVDWEMVTYWALVGTSHALHYYFALRQREIAAAQLEAR
                     LAEARLQGLQRQLHPHFLFNTLHGISALMHRDLAAADRMLALLGDLLRSSLKVTAQEI
                     SLKDELELLGKYLEIEQIRFRDRLTVTYDIPGETLDARVPSLVLQPLAENAIDHGIAP
                     TAGHGRLEIWARRHGSQLWLEVRDNGVGLRGDTQAGDLVTASGIGVSNTRSRLQCLYG
                     AAHQFEFCRVGQRGLTVRLVIPWREAPPAAAVPAVGELAS"
     gene            2631..>3444
                     /locus_tag="PKK95_15595"
     CDS             2631..>3444
                     /locus_tag="PKK95_15595"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC19692.1"
                     /translation="MRASRVTALLAAALLLGLAPTAAAQVQRGAIFGIVTDASGAVLP
                     GAVVQLTSPLTGTREAVAGSEGEFRFDNLDPGRYTLRATLAGFTPLVRENVVVGVGTT
                     VDLRMEMSVAGVQEEVKVTAETPTLDVRTQGNVANFDQQMLNEVPTARDPWVLLQFVP
                     GVQLDRINVGGSESGQQSVFAARGDDGSNTMWNVDGVTITDPAAIGSSPTYYDYSTFE
                     EVQFTTSGLDPRQQTAGLGINFVTKRGTNDFHGQARMYFTNHDLQAANIPSDL"
BASE COUNT          556 a         1216 c         1219 g          453 t
ORIGIN      
        1 cccacgcccg tccgccggcc agcaccaacg cgcacgcaaa cacgaggaag caacgggggg
       61 ccatgtttct cctcgctttc gcgatccgcg cgccgcgatt gccgcggcgc ttctgactgg
      121 atagacggac gggcgcgccc cccgccgaga gaatgtgacc aacggtgcgg agacagtgat
      181 ggagagcgag aagcgaggga tggggggcgc tgctctagat cgctccgccg aggcgcgact
      241 gcagcttgtc gcggtagccg cggctgagcg tcagccgggc gcccgtgcga agcacgacct
      301 cgtactcacc gttgaagagg ggatgcatct ccttgatccg atcgatgttc acgatctgcg
      361 accggtggat gcgcacgaag gtggagggat cgagctgttc ctccatcagc gacatcgtct
      421 ggcggacgag gtacgactcg gcgccgacgt gcagcttcag gtagttgccg gacgcctcga
      481 tccagtcgat ctcgcgggtg cggaggaacg acacgcgccc ggcgctcttg acgacgagcc
      541 ggtcggtcgc cggggggcgc tcggggcgga actcggtggc cagcgcgagc acccggcgcg
      601 cgagcgcatc gctctcctgg tgcgtcagct cggtccggac gcgctggagc gtctgctgga
      661 agcgggcctg gtcgaacggc ttgagcaggt agtcgagggc gcgcacctcg aaggcgcgca
      721 gcgcgtaggc ctcgtaggcg gtgacgaaca ccacgagcgg gaggcgctcg ccgccgaccg
      781 cctccatcac ggcaaacccg tcccgctcgg gcatctgcac gtcgaggaac accaggtgcg
      841 gcgcctcgcg ccggacggcc tcgatggccg accgcccgtc gccgcactcg gcgaccacct
      901 gcacgtccgc ctggccggcc agcagcgaaa cgatccgctc gcgcccgagc ggctcgtcgt
      961 ccacgacgat cgtcctgatg gtcatgacgc caactccccg accgccggaa ccgcggccgc
     1021 cggcggtgct tcgcgccacg ggatgacgag acgcacggtg aggccccgct ggccgacccg
     1081 gcagaactcg aactgatgcg ccgcgccgta caagcactgc aggcgcgacc tggtgttcga
     1141 gacgccgatg ccggacgccg tcaccaggtc gcccgcctgc gtgtcgccgc gcagcccgac
     1201 accgttgtcg cgcacctcga gccagagctg gctcccgtgc cgcctggccc agatctcgag
     1261 gcggccgtgc cccgcagtgg gggcgatgcc gtggtcgatc gcgttctcgg ccagcggctg
     1321 aaggacgagg ctgggcacgc gcgcgtccag cgtctcgccg ggaatgtcgt atgtgaccgt
     1381 tagacggtcg cggaagcgga tttgttcgat ctcgaggtac ttgccgagca gctcgagctc
     1441 gtccttgagc gagatctcct gcgcggtgac cttcagcgag ctgcgcagca ggtcgccgag
     1501 cagggcgagc atccggtcgg ccgcggccag gtcgcggtgc atgagcgccg agatgccgtg
     1561 cagcgtgttg aacaggaagt gggggtgcag ctggcgctgc agcccctgga ggcgcgcctc
     1621 ggccagccgg gcctcgagct gcgcggccgc gatctcgcgc tgccggagcg cgaagtagta
     1681 gtgcagcgcg tggctggtcc ccaccagcgc ccagtacgtc accatctccc agtcgaccga
     1741 catccagtag ctgctccgca cgtccgacag ccacctcgcc cacgacagcc tgccggtcag
     1801 cgcgcccctg acgagcgaca gcgtcatgac gtgcacgagc gaggtcagcg ccgcggccgg
     1861 caggtgggcg aggaggggcc gccagatcgc gcggcgtccg aagcggaagc ggcgcgccat
     1921 ccagaagacg aacggcgtca acgcggccca cgcccaccag tacgcgaggt tcagcgggaa
     1981 cgtgtgccac cacgagacgg gctcggagct ggcgaggcgg aagctgtagg cgagcagggc
     2041 cgagaacagg ccgagcagcg aggcgacagc gaggacgagg agaacgcggg gggctgcgct
     2101 tctcatgccc cactatcggg tgcaagcggc gctcccgtca aggagggaaa ccgcgacgac
     2161 atccggattt ccggacggca cgatccacgc ggttgaatgc ccgccaggcc gcgccgatcc
     2221 gcagcgttca cccgcatcgc ggatacaccg ttcgctcgtc acgcgcaccc gaccgctcgt
     2281 ccctcgccgg ggtgcgcccg tcacagagcg gcgcgcgccc gcgagccgac gccggcatcc
     2341 ggggaacggc ggcaaatcgc ggcaccccgg ttgccttgtt gtctgctgcg ggcggtgggc
     2401 gtccgcacac cggcggacag gatctcggcg gcccggctcg cgacgggcac gccccccgcg
     2461 cccagtgcac tcacaggcgg gacgcctgtg ccactcacag gcgggacgcc tgtgctactc
     2521 acaggcggga cgcctgtgcc actcacaggc gagacgcctg tgctactcac aggcgagacg
     2581 cctgtgctac tggacgcggt gcccgggatc cctcccgagg gaggtagagc atgagagctt
     2641 cacgcgtcac ggccctcctg gccgccgcgc tgctcctggg cctcgcgccc acggcggccg
     2701 cgcaggtcca gcggggcgcc atcttcggca tcgtcaccga cgccagcggc gccgtcctgc
     2761 ccggcgccgt cgtgcagctg acgtccccgc tgacggggac ccgcgaggcg gtcgccggca
     2821 gcgagggcga gttccgcttc gacaacctcg accccggccg ctacaccctg cgcgccacgc
     2881 tcgccggctt cacgccgctc gtgcgcgaga acgtggtcgt cggcgtcggc accaccgtgg
     2941 acctgcggat ggagatgagc gtggccggcg tgcaggaaga ggtcaaggtg acggccgaga
     3001 cgccgacgct cgacgtccgg acgcagggga acgtcgccaa cttcgaccag cagatgctga
     3061 acgaggtgcc gacggcgcgc gacccgtggg tgctgctgca gttcgtgccg ggcgtgcagc
     3121 tcgatcgcat caacgtcggg gggtcggaga gcggccagca gtcggtcttc gccgcgcgcg
     3181 gcgacgacgg ctcgaacacg atgtggaacg tggacggcgt gacgatcacc gacccggccg
     3241 cgatcgggtc gagccccacc tactacgact acagcacctt cgaggaggtg cagttcacca
     3301 cgagcggcct cgacccgcgc cagcagacgg cggggctcgg gatcaacttc gtgaccaagc
     3361 gcggcacgaa cgacttccac gggcaggccc gcatgtactt caccaaccac gacctgcagg
     3421 ctgccaacat cccgtccgac ctcg
//
LOCUS       DAOPQX010000392         3437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_29825_length_3437_cov_3.744530, whole genome shotgun sequence.
ACCESSION   DAOPQX010000392 DAOPQX010000000
VERSION     DAOPQX010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29825_length_3437_cov_3.744530"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /gene="argH"
                     /locus_tag="PKK95_15600"
     CDS             complement(<1..938)
                     /gene="argH"
                     /locus_tag="PKK95_15600"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOC19693.1"
                     /translation="MTNLWSGRFAGEPDRVVFEFGRSLPFDRRLFEDDVTGSLAWVEA
                     LAEAGSMDAEDAARVTAALHRILERFRDEPALAEAPDEDVHSLVERLLVEEVGDAGRR
                     LHTGRSRNDQVSLDLRLYLRRRIPLLQRAVARLAAALVDQAEKAGTSLMPAFTHVRRA
                     QPVLAAHYLLAHVAALRRDHDRLGAARDEADALPLGSGAVAGTTVPVDVDALARRLGF
                     SRVVANSVDASSDRDFVSAFVHAAALVMVHLARLAEDFVLFTGEEFGLFELADEVSTG
                     SSMMPQKKNPDPLELVRGKSGRVIGTLAGFLATMKGL"
     gene            complement(958..1365)
                     /locus_tag="PKK95_15605"
     CDS             complement(958..1365)
                     /locus_tag="PKK95_15605"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19694.1"
                     /translation="MPIDQAILEILSHESVSNQRALQGHLARRGISLTQSALSRRLRK
                     LRVHKRDGRYVKPEPPPPVAPSYTLATAPPNLVVLRTEPGFAQALALVVDHASPPGFA
                     GSVAGDDTVFFAAASPDALTPLLRSIDRLLRNR"
     gene            complement(1444..2496)
                     /gene="argC"
                     /locus_tag="PKK95_15610"
     CDS             complement(1444..2496)
                     /gene="argC"
                     /locus_tag="PKK95_15610"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOC19695.1"
                     /translation="MRLDVAILGASGFGGGELLRLLSAHPAVGSLRGTSRRFAGRPWH
                     AAHPNLRGVADGVFEAAIDWREFASSPQPVVFSAMPHGALASRLDEMEAEWASLGLAN
                     RLTLVDLSGDFRLSSAEAFALAYGAAHPCPRRLGSFVYGLPEWTRAELPGARRIAAAG
                     CFATALQLGLLPLEGLDLGLVTATAITGSSGSGATATETTHHPTRAHDIRAYKVLRHQ
                     HLPEVQATLTRGRITADLSFVPQSGPTVRGIFAVLQFGLPHGLNAEGLAARAARACAG
                     QPFLRLVEGSPRVAAVVGSNFADLAVAATDRHGAVLVAIDNLMKGMAGQAVQCLNLAL
                     GLAETTGLKYAGAYPA"
     gene            complement(2493..>3437)
                     /locus_tag="PKK95_15615"
     CDS             complement(2493..>3437)
                     /locus_tag="PKK95_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19696.1"
                     /translation="VRGEKSTYNHLEDVHGRMTVLHDGDVIAGALGHRNALHGYEGVV
                     PDTVSAGDVLNILNLGGVIGRCTSRNPEVGPPFEIELIGQVLAYPEFQSRQPQPASIG
                     LAALTGRNGVPVPPVVYVLGTCMNAGKTAAASTIVRAFSQAGFRVGGAKLTGVSLMRD
                     ILSMADYGAEVALDFTDAGVVATSAGNACGVAHAIFSELAARGMDVIVAETGDGIMGE
                     YGVQAILSDPVLRGRGRAFVLCANDPVGVAGGVTMLRERFGIQVDVVAGPATDNRVGV
                     RFVQREFGLPAINARTQARALGEAILERLALPAREGAR"
BASE COUNT          491 a         1211 c         1268 g          467 t
ORIGIN      
        1 agccccttca tcgtggccag gaagcccgcg agggtcccga tgacgcggcc agacttcccc
       61 cgcaccagct cgagcggatc ggggttcttc ttctggggca tcatgctgct gccggtgctc
      121 acctcgtctg ccagctcgaa cagcccgaac tcttcgccgg tgaacagcac gaagtcctcg
      181 gccaggcggg ccaggtgcac catcacgagc gccgccgcgt ggacgaaggc ggacacgaag
      241 tcccggtccg acgaggcatc cacgctgttg gcgaccacgc gcgagaagcc gagcctcctg
      301 gcgagcgcgt cgacgtccac cgggaccgtc gtcccggcca cggcgcccga gccgagcggc
      361 agcgcgtcgg cctcgtcgcg cgcggcgccg agccgatcgt ggtcgcgccg cagcgcggcc
      421 acgtgggcga gcaggtagtg ggccgccagt acgggctggg cgcggcgcac gtgggtgaaa
      481 gccggcatca ggctggtgcc ggccttctcg gcctgatcga cgagtgcggc ggcgaggcgc
      541 gccaccgcgc gctgcaggag cgggatgcgc cggcggaggt acagcctcag gtcgagcgac
      601 acctggtcgt tgcgcgagcg gcccgtgtgc aggcggcggc ccgcgtcgcc cacctcctcg
      661 accagcaggc gctcgacgag cgagtgcacg tcttcgtcgg gcgcctcggc gagcgcaggt
      721 tcgtcgcgga agcgctccag gatgcgatga agggccgcgg tcaccctcgc cgcgtcttcg
      781 gcgtccatgc ttcccgcttc ggcgagcgcc tccacccacg cgaggctgcc cgtgacgtcg
      841 tcttcgaaca gccgccggtc gaagggcagt gagcgcccga attcgaagac cacgcgatcg
      901 ggttcgccgg cgaaccggcc gctccagagg tttgtcaccg tgcacctccc ggccgggtca
      961 gcggttgcgc aagaggcggt cgatggatcg cagcagcggc gtcagcgcgt cggggctggc
     1021 ggccgcgaag aagaccgtgt cgtcgccggc aaccgagccg gcgaaccccg gtgggctggc
     1081 gtgatcgacg acgagcgcca gcgcctgggc gaagccgggc tcggtgcgca ggacgaccag
     1141 gttgggcggc gcggtcgcca gcgtatagga aggcgccacc ggtggcggcg gctccggctt
     1201 gacgtatcgc ccgtcgcgct tgtgcacgcg aagcttccgc agccggcgcg agagggccga
     1261 ctgggtgagc gagatgccac gccgggcgag gtgtccctgg agcgcgcgct ggttgctgac
     1321 cgactcgtgc gagaggatct cgaggatggc ctgatcgatg ggcatgatcg tgcacgcttg
     1381 ccgcggccgc ccgtggcgta ccacagatcg cttccgcgcc gccgcggcgt cgatgtgaag
     1441 acctcatgcc gggtacgctc ccgcgtactt gagcccggtg gtttcggcaa ggccgagggc
     1501 gaggttgagg cactggaccg cctggccggc catgcctttc atcaggttgt cgatcgcgac
     1561 gagcacggcg ccgtggcgat cggtcgcggc gacggcaagg tcggcgaagt tgctgccaac
     1621 cacggcggca accctcggcg atccctccac cagccgcagg aacggctgcc cggcgcaggc
     1681 acgcgcggcg cgcgccgcca gcccttcggc attgaggccg tggggaaggc cgaactgcag
     1741 cacggcgaag atgccgcgca ccgtggggcc gctctggggt acgaacgaca ggtcggctgt
     1801 gatgcgcccg cgggtgagcg tggcctgcac ctcggggagg tgctggtgtc ggagcacctt
     1861 gtaggcgcgg atgtcgtggg cccgcgtggg gtggtgcgtc gtctcggtgg ccgtggcgcc
     1921 gctgcccgac gagcccgtga tggcggtcgc ggtgaccagc cccaggtcga gcccttcgag
     1981 cgggaggagc cccagctgca gcgccgtcgc gaagcacccc gccgcagcaa tccgccgggc
     2041 gccgggcagt tccgcccgcg tccactcggg caagccgtac acgaacgatc ccaggcgccg
     2101 gggacacggg tgggcggcgc cgtaggcgag tgcgaacgct tcagcactgg aaaggcggaa
     2161 gtctccgctc aggtccacga gcgtcaggcg attggccagg ccgaggctgg cccactcggc
     2221 ttccatctcg tcgaggcggc tggccagcgc gccatggggc atggcgctga acaccacggg
     2281 ttgcggcgac gaggcgaact cgcgccagtc gatcgccgcc tcgaagacgc cgtctgccac
     2341 gccgcgcagg ttggggtgcg ccgcatgcca cgggcgcccg gcaaagcggc gcgatgtccc
     2401 ccggaggctc ccgacggccg ggtgggccga cagcagccgc agcagctcgc cgccgccgaa
     2461 tcctgaagcg ccgaggatgg cgacgtcgag tctcatcgtg ccccctcgcg ggcgggaagc
     2521 gcgagccgct cgaggatggc ctcgccgagc gctcttgcct gcgtccgcgc attgatcgcg
     2581 gggaggccga actcgcgctg gacgaaccgg accccgacgc ggttgtcggt tgccggtccg
     2641 gccaccacgt ccacctggat gccgaaccgc tcgcgcagca tggtgacgcc gccggcaacg
     2701 ccgaccgggt cgttcgcgca cagcacgaag gcgcggcccc ggccgcgcag caccgggtcg
     2761 gagaggatgg cctgcacccc gtactcgccc atgatgccgt cgcccgtctc ggccacgatc
     2821 acgtccatgc cccgggcggc gagctcgctg aagatcgcgt gcgcgacgcc gcacgcgttg
     2881 ccggcgctgg tggcgacaac cccggcgtcg gtgaagtcga gggcgacctc cgctccgtag
     2941 tcggccatcg acaggatgtc gcgcatcagc gacacgccgg tcagcttggc cccaccgacc
     3001 ctgaatcccg cctggctgaa cgcccggacg atcgtgctcg ccgcggccgt cttccccgcg
     3061 ttcatgcagg tgccgaggac gtacaccacc ggcggcaccg gcaccccgtt gcgccccgtc
     3121 agcgcggcga gcccgataga cgccggctgc ggctggcgcg actggaactc ggggtaggcg
     3181 agtacctggc cgatcagctc gatctcgaag ggcgggccga cctccgggtt gcgtgaagtg
     3241 cagcgcccga tgaccccgcc gaggttgagg atgttgagga cgtctcccgc cgagacggtg
     3301 tcgggcacca cgccctcgta gccgtgcagc gcgttgcggt ggcccagcgc cccggcgatc
     3361 acgtcgccgt cgtgcaggac cgtcatccgg ccgtgcacgt cctcgaggtg gttgtaggtg
     3421 gatttctcgc cccgcac
//
LOCUS       DAOPQX010000393         3394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_30328_length_3394_cov_3.970051, whole genome shotgun sequence.
ACCESSION   DAOPQX010000393 DAOPQX010000000
VERSION     DAOPQX010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30328_length_3394_cov_3.970051"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..661
                     /locus_tag="PKK95_15620"
     CDS             95..661
                     /locus_tag="PKK95_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOC19697.1"
                     /translation="MHPQLDAIVAEFRDGSDRLHRLADAIPGDEWGRRPRPEAWSVGE
                     CVAHLNLTTAAFRPLVEKALNEARRMPKGRVPRRYSRGLIGGLLWRALSRPGRFKTKT
                     AAAFVPGGAGSLQETMQEFDRLQDEQIGWVRAADGLPITAVRLVSPFDRRVRYNLFAA
                     LSILAAHQHRHLAQAEEGSRVTRSTRLR"
     gene            658..1281
                     /locus_tag="PKK95_15625"
     CDS             658..1281
                     /locus_tag="PKK95_15625"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOC19698.1"
                     /translation="MTPITPTFFETPEAFRRWLEQHHASERELWVGFYKVKSGKGGMV
                     YRQALDEALCYGWIDGLLRSIDEESYAQRFTPRKKDSTWSRVNVTRAQELLAEGRMRP
                     PGIEAFERRPDDKSGRYSFEQQGARLPLAYERTFKASPEAWAFFSAQPPSYRRVVTWW
                     VMSAKKEETRTRRLLQLISDSAAGQRIRQMPGAQPAVSQKPSRHPEP"
     gene            1460..1993
                     /locus_tag="PKK95_15630"
     CDS             1460..1993
                     /locus_tag="PKK95_15630"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HOC19699.1"
                     /translation="MALVAFLRGVNVGGHRTFRPTVLARALASYDVVNIGAAGTFVVR
                     KPGARAAFRAALLRALPFDTEVMLCDARDLARIVAKNPFGAGLRPPDVVWFVSILSRP
                     VRPRMELPAAFPSDDEWLVRVVASEGPFVFGMYRRAPKTIRYLGEIDKVFDVSATTRN
                     WNTILAVARVLRGRPGL"
     gene            2078..2872
                     /locus_tag="PKK95_15635"
     CDS             2078..2872
                     /locus_tag="PKK95_15635"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HOC19700.1"
                     /translation="MQRREFLKTSSLTGAAALTGLGGGTVRAAEGPREFYELRVYEMP
                     TGNRKARLNDYLAKAAIPAWNRAGSGPVGVFTVLSGANGLMLYVLVPHPSLEAFLAAP
                     EKLEADPDYKKASAEYAGRTIDDPAYTRYETWLLRAFANVPRLRVPAETAEKKPRLFE
                     LRIYESHSEAAARKKIEMFNEGGEIALFDRIGLRSVFFGQTLAGPRQPNLVYMTVHRD
                     MAAREAVWDAFRSDDGWKRLSAEPAFANTVSATTVIFLRPAAYSQI"
     gene            2970..3125
                     /locus_tag="PKK95_15640"
     CDS             2970..3125
                     /locus_tag="PKK95_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19701.1"
                     /translation="MLSVRDLRFSYGAIPVLHRVSFYQRTYRVGEEQALGLRRAQWPD
                     WTADAGF"
     gene            3166..>3394
                     /locus_tag="PKK95_15645"
     CDS             3166..>3394
                     /locus_tag="PKK95_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOC19702.1"
                     /translation="MGRLAGKRAIVTGATSGMGRATALLFAREGASVLVNGRDAERGR
                     TLVEAIRAAGGRAEFVAADATSPGTGDRLVEA"
BASE COUNT          499 a         1148 c         1211 g          536 t
ORIGIN      
        1 gggctggtgt catatggacc cgctcccgag tgtacgccaa tcgccgcggc cgagcaggcg
       61 caacaccttc gcgtgctaac ctgaccaggc cgacatgcat ccacagctcg acgccatcgt
      121 cgcggagttc cgtgatggtt ctgatcgcct gcaccggctg gccgacgcga tcccgggaga
      181 cgaatgggga aggcggccgc ggcccgaagc ctggtcggtg ggagagtgcg tcgcccacct
      241 gaacctcacg accgccgcct tccggccgct cgtggagaag gcgttgaacg aggcccgccg
      301 gatgccgaaa ggccgcgtgc cgcgccgtta ctctcgaggg ctgattgggg ggctgctctg
      361 gcgagcgctg tcgaggcccg gccggttcaa gacgaagacg gcggcggcct tcgtgcctgg
      421 cggcgccggc tcgctgcagg aaacgatgca ggagttcgat cgcctgcagg acgaacagat
      481 cgggtgggtc cgggccgctg acggccttcc cattaccgcg gtccggctcg tgagcccctt
      541 cgatcgccgc gtaaggtaca atctcttcgc cgctctgtcg atcctcgcgg cccaccagca
      601 ccggcatctc gcgcaggcag aagaaggctc gagggtcact cggtctacga ggcttcgatg
      661 accccgatta ctccgacgtt cttcgagacg cccgaggcat tccggcggtg gctggagcag
      721 catcacgcca gcgagaggga gctgtgggtc gggttctaca aggtgaagtc tggcaagggc
      781 ggcatggtgt atcgccaggc tctcgacgag gcgctgtgct acggctggat tgacggcctg
      841 ctgcgcagca ttgacgaaga gagttacgcg cagcggttca cgccgcgcaa gaaggacagc
      901 acgtggagcc gggtcaacgt gacgcgcgcg caggaactcc tggccgaggg acggatgcgg
      961 ccgccgggca tcgaagcctt cgagcgccgc ccagacgaca agtccgggcg ctactcattc
     1021 gaacagcagg gcgcacggct gccgctcgcg tacgagcgaa ccttcaaggc cagcccggag
     1081 gcctgggcct tcttctccgc gcagccgccg tcctaccgtc gggtcgtcac ctggtgggtg
     1141 atgagcgcca agaaggaaga aacgcgcacg cggcggctgc tgcagctgat ttccgactcg
     1201 gcggccgggc aacggatccg gcagatgcct ggtgcccaac ctgccgtctc gcagaaaccg
     1261 tctcgccacc ctgagccctg agcctcacgc gcgacgggcc cttcctcggt gctggtggac
     1321 gcggacggcg agctggtcgg ccgcctgccg gccacttacc gcgtcgtccc tggcgccctg
     1381 gacgtgtgtg tgccggtctc ccgggtcgcg ggcctggctt ccggcagcca ggcgagcgct
     1441 tcgggttagc atgttcgatg tggctctcgt ggccttcctg cgcggcgtca acgttggcgg
     1501 gcacaggacg tttcgcccga cggtgctcgc gcgggctctg gcctcctacg acgtcgtcaa
     1561 catcggcgcc gccggcacct tcgtggttcg caagcccggt gcgcgcgcgg cgtttcgggc
     1621 cgcgctgctc cgggcgctgc cgttcgacac cgaggtgatg ctctgcgacg cccgcgatct
     1681 cgcgcgcatc gtcgcgaaga acccgttcgg cgccggcctg cggccaccgg acgtcgtctg
     1741 gttcgtgagc atcctgtcga ggcccgttcg cccgcggatg gagcttccgg ccgcgttccc
     1801 ctccgacgac gagtggctcg tgcgggtggt ggcatcggag gggccgttcg tgttcgggat
     1861 gtaccgccgc gccccaaaga cgattcggta cctcggcgag atcgacaagg tgttcgacgt
     1921 gtcggccacc acccggaact ggaacacgat cctggcggtc gcgcgcgtgc tccgggggcg
     1981 gccggggctg tgacctcgtg acttcgcgac ctcgacccgc tagacttcgt gcacctcggg
     2041 gtcttgtcac cttcaaccac cgacaacggg ggccgtcatg cagcgtcgcg agttcctgaa
     2101 gacatcgtcg ctcaccggcg ccgccgcgct cacggggctc gggggaggga cggttcgcgc
     2161 ggccgagggg ccgcgcgagt tctatgagct gcgcgtgtac gagatgccca cggggaaccg
     2221 caaggcgcgg ctcaacgact acctggcgaa ggcggcgatt ccggcgtgga accgcgccgg
     2281 gagcggcccc gtcggcgtgt tcaccgtcct gtcgggcgcc aacgggctga tgctctacgt
     2341 gctcgtcccg cacccgagcc tcgaggcgtt tctcgccgcc ccggagaagc tggaggcgga
     2401 cccggactac aagaaggcgt cggccgagta cgcgggccgc acgatcgacg accccgccta
     2461 cacccgctac gagacctggc tgctgcgcgc gttcgcgaac gttccccgtt tgcgcgtccc
     2521 ggccgagacg gcggagaaga agccgcgcct gttcgagctg cgcatctacg agagccacag
     2581 cgaggcggcg gcccgaaaga agatcgagat gttcaacgag gggggcgaga tcgccctctt
     2641 cgatcgcatc ggcctgcggt cggtcttctt cgggcagacg ctcgccgggc cccggcagcc
     2701 gaacctggtc tacatgaccg tgcaccgcga catggctgcc cgcgaggccg tctgggacgc
     2761 cttccgcagc gacgacgggt ggaagagact gagcgccgag ccggcgttcg ccaacaccgt
     2821 gtcggcgacg accgtgatct tcctgcgccc ggcggcgtat tcgcagatct gagtctcgtc
     2881 tggcagctgc gcatgccgcg gctgctccgc gctcgccggc ggcccgttcc tggcgtacct
     2941 cgtcagggag cacgcggccg gatagcgaca tgctctcggt acgcgacctc cgtttctcgt
     3001 acggcgcgat ccccgtgctg caccgcgtct cgttctacca gcgcacctac cgagtcggcg
     3061 aggagcaggc gctcgggctg cgccgcgcgc agtggccgga ctggacggcg gacgcgggct
     3121 tttgagcgcg tcctgcggta gactcgggcc tcaccggggg gacgcatggg ccgactggcg
     3181 gggaagagag cgatcgtcac gggagccacg agcggcatgg gacgggcgac ggccctcctg
     3241 ttcgcacgcg aaggggcctc ggttctggtc aacggccgcg acgccgagcg agggcgcacg
     3301 ctggtcgagg cgatccgtgc ggcgggggga cgtgccgagt tcgtcgccgc cgatgcgacg
     3361 agtcccggga cgggcgaccg gttggtggaa gcct
//
LOCUS       DAOPQX010000394         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_30760_length_3357_cov_3.314658, whole genome shotgun sequence.
ACCESSION   DAOPQX010000394 DAOPQX010000000
VERSION     DAOPQX010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30760_length_3357_cov_3.314658"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PKK95_15650"
     CDS             complement(<1..1323)
                     /locus_tag="PKK95_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19703.1"
                     /translation="MTKSRIATLCLAVLVLGLASAAEAQIDARLFRQPAISATHIAFV
                     YAGDIWVVQKQGGVALRLSSPRGEEQFPRFSPDGSRIAFSANYDGNTDVYVVPALGGD
                     PVRVTHHPMADRVLGWHPDGKRVLFASGRESGRQRYSQFFLAPADGGEATRLMVPYGE
                     FGSFSPDGGQFAYMPMSQDFRTWKRYRGGWAPDIWLFDLKTFAARNLTRNEANDAHPM
                     WFGEQLYFMSDRGPEKRQNLWVLDMKSGATRQVTAFRDYDVTFPSAGPTDIVFQNGGR
                     LYVLDVAGGGQPREVKVQVVTDRATLKPHVARVEALLRAPSVSPTGKRAAFEARGDLF
                     SIPAEHGPVVNLTNTSGVAERYPRWSPDGKTLAYWSDRSGEYELTFRAADGAGAEETV
                     TKLGPGFRYRVSWSPDSRKLAFIDQAMRIWLYDRDVKKPVRIDTSTTWI"
     gene            complement(1444..2238)
                     /locus_tag="PKK95_15655"
     CDS             complement(1444..2238)
                     /locus_tag="PKK95_15655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOC19704.1"
                     /translation="MKGTALVTGASSGIGAELARVFAANGFDLVLTARRLDRLEALAA
                     ELLDRHQCRVHVIAADLADPSSPSRIAREVAEAGFQVDALVNNAGYGLPGSFLSSPWE
                     LHRAFIQVMVTAVAELCRLFAPGMVERRRGWIINVGSVAGLVPAAAGHTLYGAVKALV
                     IRFSESLSCELRPHGVHVTALCPGFTYSEFHDVNRMRAQVSRLPSFLWLHADRVAREG
                     YEAVMRGDPVFVNGRVYRAIVLGARLAPSWLVNLATRKMAPRFRRT"
     gene            complement(2300..>3357)
                     /gene="gltS"
                     /locus_tag="PKK95_15660"
     CDS             complement(2300..>3357)
                     /gene="gltS"
                     /locus_tag="PKK95_15660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HOC19705.1"
                     /translation="AFFTTIGLGASLGLLRIGGPQVLLFWVLASVLAVFQDAVGVGLA
                     RLLGVHPFLGLIAGSITMTGGHGTGAAFGKLMEDQYAFPGAVTLAMAAATFGLVSGGL
                     LGGPIATRLVRRRSLAPRPAAQAAPAPAAVAEYQPLDQEIDTEPAGAAPTAYTLLKII
                     TITLVAMWAGAALGSWIQGTTGITLPAYIGAMIVAAVLRNADDGVAALTKGRGGRKAG
                     LFGIEQRVVDDVGTVALSLFLSMALMSLKLWELLDLAIPMLVILTAQVTMMGLYATFV
                     TFWLMGRDYDAAVMAGGHCGFGLGATPNAVANMEAITERFGAAPRAFLVLPLVGAFFI
                     DFTNAIIITTFLNLVRP"
BASE COUNT          553 a         1170 c         1176 g          458 t
ORIGIN      
        1 gatccaggtg gtgctcgtgt cgatgcggac cggcttcttc acgtcgcggt cgtagagcca
       61 gatgcgcatc gcctggtcga tgaacgccag cttgcggctg tcgggcgacc aggagacgcg
      121 gtagcggaag ccgggaccga gcttggtgac ggtctcctcg gcaccagcgc cgtcggcggc
      181 gcggaaggtc agctcgtact cgcccgagcg gtcgctccag tacgccagcg tcttcccgtc
      241 aggcgaccag cgcgggtagc gctcggccac cccggacgtg ttggtgaggt tcacgaccgg
      301 gccgtgttcg gccggaatcg agaacaggtc gccgcgggcc tcgaaggcgg cacgcttgcc
      361 ggtgggcgag accgaggggg cgcggagcag cgcctcgacc cgggcgacgt ggggcttgag
      421 ggtcgcacgg tcggtgacca cctgcacctt cacctcgcgc ggctggcctc cgccggccac
      481 gtcgagcacg tacaaccgcc cgccgttctg gaagacgatg tcggtcggac cggccgaagg
      541 aaacgtgacg tcgtagtcgc gaaacgccgt cacctggcgg gtggcacccg acttcatgtc
      601 gagcacccac aggttctgcc gcttctcggg gccgcggtcg gacatgaagt acagctgctc
      661 gccgaaccac atcgggtggg cgtcgttggc ctcgttcctg gtcaggttgc gggcagcgaa
      721 cgtcttcagg tcgaagagcc agatgtcggg ggcccacccg ccgcggtagc gcttccaggt
      781 tcgaaagtcc tgcgacatcg gcatgtaagc gaactgcccg ccgtcgggcg agaacgatcc
      841 gaactcgccg tagggcacca tgagcctggt cgcctccccg ccgtcggcgg gagccaggaa
      901 gaactggctg tagcgctggc ggccgctctc ccggcccgag gcgaacaaga cccgcttgcc
      961 gtccgggtgc cacccgagca cgcggtcggc catcgggtgg tgagtcaccc gcaccgggtc
     1021 gccgccgagc gccggcacga cgtagacgtc ggtgttcccg tcgtagttgg cgctgaaggc
     1081 gatgcgcgac ccgtcgggcg agaagcgcgg gaactgctcc tcgccgcgcg gcgacgacag
     1141 gcgcaaggcc acgccgccct gtttctggac gacccagatg tcgccggcgt agacgaaggc
     1201 aatgtgcgtg gccgagatgg ccggctggcg gaacagccgg gcgtcgattt gcgcctccgc
     1261 ggccgaagcc aggccgagca cgagaactgc gagacacagc gtcgcgatgc gtgatttggt
     1321 catgagaagc tcctgccggc tcgcggccgc cttcaagcct gccctactgc gctccgccgt
     1381 ttcgctgcct gctgtcgttg ctttgtggtg cccgtttgcc gcgggcgacc acaagggtcg
     1441 cccctacgtc cgccgaaacc ggggcgccat cttcctcgtg gccaggttca cgagccacga
     1501 gggcgcgagg cgcgcgccca ggacgatggc gcggtagacg cgcccgttga cgaagacggg
     1561 gtcgccgcgc atcaccgcct cgtatccctc gcgggccacg cggtcggcgt gcagccacag
     1621 gaacgacggc agccggctga cttgcgcccg catccggttc acgtcgtgga actcgctgta
     1681 cgtgaagccc gggcagagcg cggtgacgtg cacgccatgc ggcctcagct cgcaggacag
     1741 cgactcggag aagcggatga cgagcgcctt cacggccccg tacagcgtat gcccggcggc
     1801 cgccggcacg agccccgcca ccgacccgac gttgatgatc cagccgcggc gccgctcgac
     1861 catgccggga gcgaacagcc ggcacagctc ggcgaccgcg gtcaccatca cttggatgaa
     1921 cgcccggtgc aactcccagg gcgaagacag gaacgaacca ggcaggccgt aaccggcgtt
     1981 gttcaccagc gcatcgacct ggaagcccgc ctccgcaacc tcgcgggcga ttcgcgaggg
     2041 tgatgacggg tcggcgaggt cggccgcgat cacgtgaacg cggcactgat ggcggtcgag
     2101 aagctctgcg gccagcgcct cgaggcggtc gaggcgccgc gcggtgagca ccaggtcgaa
     2161 gccgttggcg gcgaagacgc gcgccagctc cgcgccgatg ccggacgagg cgcccgtgac
     2221 gagtgctgtt cctttcaatc gcgctgcctc cagcccacgg tcaccgcagt gaccgagaac
     2281 gcgcgccacg gagccgcccc tacggccgca ccaggttgag gaacgtcgtg atgatgatag
     2341 cgttcgtgaa gtcgatgaag aaggcgccca ccaggggcag cacgaggaag gcacgcggcg
     2401 ccgccccaaa acgctccgtg atcgcctcca tgttggccac ggcgttcggc gtcgccccga
     2461 ggccgaaccc gcagtgcccc ccggccatga ccgcggcgtc gtagtcacgc cccatgagcc
     2521 agaaggtgac gaaggtggcg tacagcccca tcatcgtgac ctgcgcggtg aggatgacca
     2581 gcatcgggat cgccaggtcg agcagttccc acagcttcag cgacatcagc gccatcgaga
     2641 ggaagagcga cagcgccacg gtgccgacgt cgtccaccac gcgctgctcg atgccgaaca
     2701 gccccgcctt ccgcccgccg cgccccttcg tgagcgctgc caccccgtcg tcggcattgc
     2761 gcagcacggc ggcgacgatc atcgcgccga tgtaggcggg gagcgtgatg ccggtggttc
     2821 cctggatcca ggagccgagc gccgccccag cccacatcgc gaccagcgtg atggtgatga
     2881 tcttcagcaa cgtgtacgcg gtgggagccg cgccggccgg ctcggtgtcg atttcctggt
     2941 cgaggggctg gtactctgcg acggcggcag gagccggcgc ggcctgcgcg gcggggcgcg
     3001 gggccaggct ccggcggcgg accaggcggg tcgcgatcgg cccgcccagc aggccgccgc
     3061 tcacgagtcc gaaggtcgcc gccgccatgg ccagcgtcac ggcaccgggg aacgcgtact
     3121 gatcctccat cagcttgccg aacgcggcgc ccgtgccatg gccgccggtc atcgtaatcg
     3181 accccgcgat gaggcccagg aaggggtgaa ccccgagcag cctggcgagc ccgacgccaa
     3241 ccgcgtcctg gaagacggcc agcaccgacg ccagcaccca gaacagcagc acctgcggcc
     3301 ctccgatgcg cagcaggccc aggctggcac ccaggccgat cgtcgtgaag aacgcaa
//
LOCUS       DAOPQX010000395         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_30792_length_3354_cov_5.268263, whole genome shotgun sequence.
ACCESSION   DAOPQX010000395 DAOPQX010000000
VERSION     DAOPQX010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30792_length_3354_cov_5.268263"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="PKK95_15665"
     CDS             complement(<1..1369)
                     /locus_tag="PKK95_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOC19706.1"
                     /translation="MSIHVGIDVGSASVKAAVYSTDPADAVFFERLVQDGGGIFELVR
                     QIEDAAGRPAWLAVARPAPLTGNPAGQARAMLDRVEAALPAGALGSVAATGSGAALLA
                     GVELRHEFQALSRATEVLLPSVRTLFEIGGESSKYIRFEPHEGRGGRRLGIADYQTNG
                     DCAAGTGAFLDQQAGRLRFRVEEIGDIVLNTRRAAQIAGRCSVFAKSDMIHAQQKGFS
                     PEEILRGLCDAVARNFRSAVVRGRPIDPEVAFVGGVASNAGVVRALEQAFKWAPGTLV
                     VPPGHQSFGAVGAAVLAHDAANEAEDDGHAAVAAVAGPDEEVPTSAPLSMANVLLLRD
                     RLQAVPPGEIRMPIDAYLGIDIGSVSTNLVVIDAERRVLKEIYLRTDARPIEAVATGL
                     RQIEQELGQLVRIRGVGTTGSGRELIGELVGADTVNDEITAHKTGAAFIDDTILGLGV
                     DTIFEI"
     gene            complement(1557..3113)
                     /locus_tag="PKK95_15670"
     CDS             complement(1557..3113)
                     /locus_tag="PKK95_15670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC19707.1"
                     /translation="METTFGGAPINNITKGLPKTVESICPEPGCGRVIDARMFAEDGK
                     VWMEKTCPEHGYVKDLYWSDVELFLKAEKWEFGDYRGLSNPFTESAECPRDCGLCQQH
                     TSHTALGNIDLTNRCNLTCPICFANANVTGSVYEPSREQVKEMLRLYRREQPVQGRVV
                     QFSGGEPTIHPDFFDILRDAQELGFSHLQIASNGIKLSDPDFAARAAEAGLHTIYLQF
                     DGLDDRVYKELRGRPMLDVKLKAVESIRRAGMKIVYVPTIARTINDDQVVPILRFAIE
                     NIDVTSGISYQPVALTGRVSHEERRRLRYTIPDLVHAISDAGYTATADWYPLSLVSPI
                     SKFISAMRDDPTVHMTCHPHCSMGTYLAIEPGSKRGVPVTQFIDVEGMFKALDERSVK
                     MANARFKIFQKAGAFNDLRSFFKPEGAPRGMDFAKFTRTLQGLLDKKVGRGPQADTYK
                     MLLVAGMHFMDGYNYELERVRRCLIHYATPAGRVIPFCAYNGGHYLRERIEQQFSVPL
                     AEYQRRRAQA"
BASE COUNT          473 a         1184 c         1159 g          538 t
ORIGIN      
        1 cgatctcgaa gatcgtgtcc accccgaggc cgaggatcgt gtcgtcgatg aacgccgcgc
       61 cggtcttgtg cgcggtgatc tcgtcgttca ccgtgtcggc gccgaccagc tcgccgatca
      121 gctcccgccc cgaccccgtc gtcccgacgc cgcggatgcg gacgagctgg cccagttcct
      181 gctcgatctg ccgcaggccg gtggccaccg cctcgatcgg ccgcgcgtcg gtgcgcaggt
      241 agatctcctt gagcacgcgc cgctcggcgt cgatgaccac caggttggtg ctcaccgatc
      301 cgatgtcgat cccgaggtag gcgtcgatcg gcatccggat ctcgcccggc gggaccgcct
      361 gcagccggtc gcggagcagc agcacgttcg ccatcgagag cggcgccgag gtcggcacct
      421 cctcgtcggg cccggcgacc gccgcgaccg ccgcgtgccc gtcgtcctcg gcctcgttcg
      481 ccgcgtcgtg ggcgagcacg gcggcgccga cggcgccgaa cgactggtgt cccggcggca
      541 cgacgagcgt gcccggcgcc cacttgaagg cctgctccag cgcgcggacc acgccggcgt
      601 tcgacgcgac gccgccgacg aaggcgacct cggggtcgat gggccggccg cgcaccaccg
      661 cgctccggaa gttccgcgcc accgcgtcgc acagcccccg gaggatctcc tcgggcgaga
      721 accccttctg ctgcgcgtgg atcatgtcgc tcttggcgaa gaccgagcag cggccggcga
      781 tctgcgccgc gcggcgcgtg ttcagcacga tgtcgccgat ctcctccacg cggaagcgca
      841 gccggccggc ctgctggtcg aggaaggcgc cggtgccggc ggcgcagtcg ccgttggtct
      901 ggtagtcggc aatgcccagg cggcgccccc cgcggccctc gtgcggctcg aaccggatgt
      961 acttggagct ttcgccgccg atctcgaaca gcgtgcggac cgagggcagc agcacctcgg
     1021 tcgcgcgcga cagcgcctgg aactcgtggc gcagctcgac cccggccaag agcgccgcac
     1081 cgctgccggt ggcggcgacc gacccgagcg cgccggcggg aagggcggcc tcgacccggt
     1141 cgagcatcgc gcgcgcctgc cccgcggggt tgccggtcag cggggccggc cgggcgacgg
     1201 ccagccacgc ggggcggccg gccgcgtctt cgatctggcg gaccagctcg aagatgccgc
     1261 ccccgtcctg gacgaggcgc tcgaagaaga cggcgtcggc cgggtcggtc gaatagacag
     1321 ccgccttgac gctcgccgaa ccgacatcga tcccgacgtg gatggacatg ccctgctctc
     1381 gccgtgccgg gggcaggaca cggtgatgtc acgcgatgac agtagacggt tgtgccgccg
     1441 gggggtgcgc accggcgcgc ccccaggcgg ccctgccaga ccccggagct caccgcggtt
     1501 ggcgtgttcg ctccggcaga aacggggctg cagcctcgtc cggcggcgcg agggtctcag
     1561 gcctgcgccc gccgccgctg gtactcggcc agcggcaccg agaactgctg ctcgatccgc
     1621 tcgcggaggt agtgcccgcc attgtacgcg cagaacggaa tcacccggcc tgcgggggtg
     1681 gcgtagtgga tcaggcagcg ccgcacgcgc tcgagctcgt agttgtagcc gtccatgaag
     1741 tgcatgccgg ccaccagcag catcttgtag gtgtcggcct gcggcccgcg cccgaccttc
     1801 ttgtcgagca ggccctggag cgtgcgggtg aacttggcga agtccatccc ccggggcgcc
     1861 ccctcgggct tgaagaacga gcgcaggtcg ttgaaggccc ccgccttctg gaagatcttg
     1921 aagcgggcgt tcgccatctt caccgagcgt tcgtcgagcg ccttgaacat cccctcgacg
     1981 tcgatgaact gggtcaccgg cacgccccgc ttcgaccccg gctcgatcgc caggtaggtc
     2041 cccatcgagc agtgcgggtg gcacgtcatg tggaccgtcg ggtcgtcgcg catcgcggag
     2101 atgaacttcg aaatcgggct caccaggctg agcgggtacc agtccgcggt ggccgtgtag
     2161 cccgcgtccg agatggcgtg gacgaggtcg gggatcgtgt agcgcaggcg ccgccgctct
     2221 tcgtggctca cgcgccccgt cagcgccacc ggctggtagc tgatgccgct ggtgacgtcg
     2281 atgttctcga tggcgaaccg caggattggc accacctggt cgtcgttgat cgtccgggcg
     2341 atcgtcggca cgtagacgat cttcatgccc gcgcgccgga tcgactccac ggccttcagc
     2401 ttgacgtcga gcatcggccg gccgcgcagc tccttgtaga cccggtcgtc gagcccgtcg
     2461 aactgcaggt agatcgtgtg caggccggcc tcggccgcgc gcgcggcaaa gtcgggatcg
     2521 gacagcttga tgccgttcga cgcgatctgc aggtgcgaga agcccagctc ctgcgcgtcg
     2581 cgcaggatgt cgaagaagtc ggggtggatg gtcggctcgc cgcccgagaa ctggacgacc
     2641 cgcccctgca ccggctgctc gcggcggtag agccgcagca tttccttcac ctgctcgcgc
     2701 gacggctcgt acaccgagcc ggtgacgttc gcgttcgcaa agcagatcgg gcacgtgagg
     2761 ttgcagcggt tggtgaggtc gatgttgccg agcgccgtgt ggctcgtgtg ctgctggcag
     2821 agcccgcagt ccctcgggca ctccgcgctc tcggtgaagg ggttgctcag gccgcggtag
     2881 tcgccgaact cccacttctc cgccttgagg aacagctcga cgtcggacca gtacagatcc
     2941 ttcacgtagc cgtgctcggg gcacgtcttc tccatccaca ccttgccgtc ctcggcgaac
     3001 atccgcgcgt cgatgacccg tccgcacccg ggctccgggc agatcgactc caccgtcttc
     3061 ggcagcccct tggtgatgtt gttgatcggg gccccgccga aggtggtttc caccccaggc
     3121 tccgattcct gcagccgtct caacataagt cgtgctccag ctggatgaca gtcctgcggc
     3181 tcactcatct cgcgcgatgc cgggggccag gccgttgacg atctggtggg ccgagcttca
     3241 ttgtcgtcgt tcctggcccg gctgtaacgg agctgcggcg aacgggcaaa tcgggtggcc
     3301 gaactggcaa aaccccgccc gaaaagccag ctgtgccgta gctttcagcc ttct
//
LOCUS       DAOPQX010000396         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_30869_length_3349_cov_3.680328, whole genome shotgun sequence.
ACCESSION   DAOPQX010000396 DAOPQX010000000
VERSION     DAOPQX010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30869_length_3349_cov_3.680328"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKK95_15675"
     CDS             <1..219
                     /locus_tag="PKK95_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19708.1"
                     /translation="ENTASRMSHFARQEIYYDRQFGLDETLEGIERVTAADVQRVAAE
                     LFRDGNLAGTVLGSLNGWRYPADRLKLA"
     gene            301..1611
                     /gene="purB"
                     /locus_tag="PKK95_15680"
     CDS             301..1611
                     /gene="purB"
                     /locus_tag="PKK95_15680"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOC19709.1"
                     /translation="MIARYTNPAMGRIWSDQRRFETWLEVEIAAAEAMAEAGIVPASA
                     ARAIRERGGFDIARIEEIEARTHHDVIAFTTAVAERVGEEARWLHFGLTSSDVIDTAQ
                     ALQMREACDLILEGLEGLADAIRRRAEEHRRTPMIGRTHGVHAEPMTFGLKLALWYAE
                     VGRQIERVRQARAGIAVGKLSGAVGTFAHLDPSVEAAVCARLGLEPAPISSQIVQRDR
                     HAALLSALALAGASLEKFALEIRGLQKTEIGEVEEPFAAGQKGSSAMPHKRNPIGCEQ
                     VVGLARLLRGNAGVAMENIALWHERDISHSSVERVILPDSFIALDHMLRRFTRIVKGM
                     VVYTERMRENLERSRGVVFSGSVLLELARKGVSREQAYEWVQRNAMRSSREALSFKDL
                     LLEDADVRGVLDATEIDRVFDLDVQLKQVDAIFARVFRPSPETV"
     gene            1679..3118
                     /gene="purF"
                     /locus_tag="PKK95_15685"
     CDS             1679..3118
                     /gene="purF"
                     /locus_tag="PKK95_15685"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOC19710.1"
                     /translation="MFDKLHEECGVFGIFGHPEAAKMAYLGLYALQHRGQESAGIAAG
                     PNGGDRLAVHRGMGYVADVFGQSELERLPGQMAVGHVRYSTAGASKLVNAQPILIDCS
                     HGQLALCHNGNLVNASELRDELVGEGAIFQTSSDSEVILHLYARSKEHRPEQALIASL
                     ARVRGAYSLVLATSDRLMAVRDPHGFRPLALGRLGGAVVVASETCAMDLIGATWERDL
                     EPGELVVVDADGVHSHKPFPPARHAFCIFEHVYFARPDSYVFGESVNEIRTAFGRQLA
                     RESAVGADVVVPVPDSGVCAATGFAEETGLPLRIGLIRNHYVGRTFIEPQQSIRHFGV
                     KVKLNPVRSILEGKRVVLVDDSIVRGTTSRKIVRMIKAAGATEVHVRISCPPTISPCF
                     YGVDTPRRSELIAATHTLEEIRKYLDADSLRYLSLEGMLSAVNSHRGHYCTSCYTGHY
                     PVPFPREQGTQAPLPLQLVNRRSPDEESR"
     gene            3115..>3349
                     /locus_tag="PKK95_15690"
     CDS             3115..>3349
                     /locus_tag="PKK95_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19711.1"
                     /translation="MIDYKSAGVDIDAGNEAVRRIISAGGTLSHIAGLDNFCWPDPVR
                     SSITPDGEYKLAQLVRANKALHDVTTAFEVPCVS"
BASE COUNT          513 a         1150 c         1182 g          504 t
ORIGIN      
        1 gagaacacgg cgagccggat gtcgcacttc gcccgccagg agatctacta cgaccgccag
       61 ttcggcctcg acgagacgct ggaggggatc gagcgggtga ccgccgccga cgtccagcgg
      121 gtggcggccg agttgttccg ggacgggaac ctggccggga cggtgctcgg atcgctcaac
      181 ggatggcggt accccgccga tcgcttgaaa ctcgcgtagc gcgccggtaa tctacgtgtt
      241 ttcaacctgc gggcccccgg cccgcggcgc cccctttctc ccgctcagcc gaggatcccg
      301 atgatagccc gatacaccaa cccggccatg ggccggatct ggagcgacca gaggcgcttc
      361 gagacgtggc tggaggtgga gatcgcggcg gccgaggcga tggccgaggc ggggatcgtc
      421 ccggcgtccg cggcgcgcgc catccgggag cggggcgggt tcgacatcgc gcgcatcgag
      481 gagatcgagg cacggacgca ccacgacgtg atcgcgttca cgacggccgt cgccgagcgg
      541 gtgggcgagg aggcgcgctg gctgcatttc ggcctcactt cctcggacgt catcgacacg
      601 gcgcaggcgc tgcagatgcg cgaggcgtgc gacctgatcc tcgaggggct cgaggggttg
      661 gccgacgcga ttcgccggcg cgccgaggaa catcggcgga ccccgatgat cgggcggacg
      721 cacggggttc atgccgagcc gatgaccttc ggactgaagc tcgccctctg gtacgcggag
      781 gtgggccgcc agatcgagcg cgtgcggcag gcccgggcgg ggatcgcggt cggcaagctc
      841 tccggtgcgg ttggcacctt cgcacacctc gacccgtccg tcgaggcggc ggtgtgcgca
      901 cgcctcggcc tggagccggc ccccatctcg tcgcagatcg tccagcgcga ccgccacgcg
      961 gcgctgctct cggccctcgc gctcgccggg gcgtcgctgg agaagttcgc cctcgagatc
     1021 cgggggctcc agaagaccga gatcggcgag gtcgaggagc cgttcgcggc ggggcagaag
     1081 gggtcgtcgg cgatgccgca caagcggaac cccatcggct gcgagcaggt ggtggggctc
     1141 gcgcggctgc tccgcggcaa cgccggggtc gcgatggaga acatcgccct ctggcacgag
     1201 cgcgacatct cgcactcctc ggtcgagcgc gtgatcctgc ccgacagctt catcgccctc
     1261 gaccacatgc tccgccgctt cacccgcatc gtgaaaggca tggtcgtcta cacggagcgg
     1321 atgcgcgaga acctcgagcg atcgcgcggg gtggtcttct cggggtcggt ccttctggag
     1381 ctggcgcgca agggagtctc gcgcgagcag gcctacgagt gggtgcagcg caacgccatg
     1441 cgatcgtcgc gggaggcgct ttccttcaag gacctgctcc tcgaggacgc cgacgtccgc
     1501 ggcgtgctcg acgcgacgga gatcgatcgg gtcttcgacc tggacgtgca gttgaagcag
     1561 gtggatgcca tcttcgcccg ggtgttccgg ccgtcacccg agaccgtgtg aggggcggcc
     1621 gccggccgct gccgctggcg cccgttggcc ggggccgcta acctgataga agggaccagt
     1681 gttcgacaag cttcacgagg agtgcggcgt cttcggcatc ttcggccacc ccgaggcggc
     1741 caagatggcc tacctggggc tctatgccct gcagcaccgg ggccaggaga gcgccggcat
     1801 cgccgccggg cccaacggcg gcgaccggct ggcggtccac cggggcatgg ggtacgtggc
     1861 cgacgtcttc gggcagtcgg agctggagcg gctgccgggg cagatggccg tgggccacgt
     1921 ccggtactcg acggcggggg ccagcaagct ggtcaacgcg cagccgatcc tcatcgactg
     1981 ctcgcacggc cagctcgccc tctgccacaa cgggaacctg gtcaacgcct ccgagctgcg
     2041 cgacgagctg gtgggcgagg gggccatctt ccagaccagc agcgactccg aggtgatcct
     2101 ccacctctac gcccgatcga aggagcaccg gcccgagcaa gccctgattg cctcgctggc
     2161 gcgggtccgc ggggcctact cgctggtgct ggcgacctcc gatcgcctga tggccgtgcg
     2221 cgacccgcac ggcttccggc cgctggcgct cggccggctg ggcggtgccg tcgtcgttgc
     2281 gtcggagacc tgcgcgatgg acctcatcgg cgccacctgg gagcgcgacc tcgagcccgg
     2341 cgagctggtg gtggtcgacg cggacggcgt ccactcgcac aagccgttcc cgccggcgcg
     2401 ccacgccttc tgcatcttcg agcacgtgta cttcgcccgt cccgacagct acgtcttcgg
     2461 cgagagcgtc aacgagatcc gcaccgcgtt cggccggcag ctggcgcgcg agtccgcggt
     2521 tggcgcagac gtcgtggtcc cggtgcccga ctccggggtg tgcgcggcca ccggcttcgc
     2581 ggaggaaacg ggcctcccgc tccgcatcgg gctgatccgc aaccactacg tcggccgaac
     2641 cttcatcgag ccgcagcagt cgatccgcca cttcggggtg aaggtgaagc tgaaccccgt
     2701 ccgcagcatc ctggagggga agcgggtcgt gctggtggac gactcgatcg tgcgcggaac
     2761 gaccagccgg aagatcgtgc ggatgatcaa ggcggccggg gcgaccgagg tgcacgttcg
     2821 catcagctgc ccgccgacga tctctccctg cttctacggc gtcgacaccc cgcggcggtc
     2881 cgagctgatt gccgccaccc acacgctcga ggagatccgg aagtacctgg acgccgactc
     2941 gctgcgatac ctcagcctcg agggaatgct cagcgccgtc aactcccatc gcgggcacta
     3001 ctgcacgtcc tgctacacgg ggcactaccc ggtgccgttc cccagggagc agggcacgca
     3061 ggcgcccctc ccgctgcagc tggtcaaccg ccgctccccc gacgaagaat cgcgatgatc
     3121 gactacaagt cagccggcgt ggacatcgac gcgggcaacg aggcggtccg ccgcatcatc
     3181 agcgcgggcg gaacactgtc ccatatcgcg ggattggaca atttctgctg gcctgatccc
     3241 gtcaggtcgt caataacgcc cgacggcgaa tacaagctgg cccagctggt tcgcgccaac
     3301 aaagcgcttc atgatgtgac caccgcattc gaggttccct gtgtttcgg
//
LOCUS       DAOPQX010000397         3348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_30882_length_3348_cov_5.366839, whole genome shotgun sequence.
ACCESSION   DAOPQX010000397 DAOPQX010000000
VERSION     DAOPQX010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30882_length_3348_cov_5.366839"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKK95_15695"
     CDS             <1..206
                     /locus_tag="PKK95_15695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOC19712.1"
                     /translation="EASVPLFEYECRKCGHHFEALVVGSRRPACPKCKSEDLEKKASA
                     FGVAGAGAWSGASSGCGTGGGGG"
     gene            complement(327..2015)
                     /locus_tag="PKK95_15700"
     CDS             complement(327..2015)
                     /locus_tag="PKK95_15700"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19713.1"
                     /translation="MRSSTCAVVLAVAAVVVAAGAPVTPQEVNVPDQAALDALARQFA
                     PVEIGADVSALPASERQALARLVEAGWVMDGLFLRQVWAGNPALLQQLLADPTALGKA
                     RLRYFLINKGPWDRLDHNRPFVPGVPPKPEGANFYPAGSTKAEVETWMQRLSGEARGE
                     AAGFFTTIRRAPGGGFQAVPYSVEYQGELARAAALLREAAASTAQPTLKRFLETRADA
                     FLSNDYYESDVAWMELDASIEPTIGPYEVYEDEWFNAKAAFEAFITLRDEAESAKLAR
                     LGGELQDIENHLPIAPAFRNPKLGSLAPIRVVNVVLAAGDGNRGVQTAAFNLPNDERV
                     VREKGAKRVMLRNVQEAKFKLVLVPISGRVLAPADRQRVTFDAFFTHILMHELMHGLG
                     PHQITAGGEQTTVRARLKETYSAIEEAKADVSGLFALQYLIDKGVLDKALQPTMYETF
                     LASAFRSIRFGLSEAHGKGIALQLNWLLDAGAFNVNPDGTFAVDHARIKDAVTRLTSE
                     LMTLQAEGDYGKADALLKRLVVLRPEVQKALDRLSGIPVDIEPRFESARRLLEN"
     gene            complement(2056..3099)
                     /gene="queG"
                     /locus_tag="PKK95_15705"
     CDS             complement(2056..3099)
                     /gene="queG"
                     /locus_tag="PKK95_15705"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOC19714.1"
                     /translation="MLTAERIKEKARAIGFDLCGIAPAGAWPELGFFREWLARGYAGT
                     MGYLPRTARVREDVRQVLPSARSVIATATLYHADRPLSVESADEEEAIISRYAWGDDY
                     HDVIGRRLEALLAWMRDQAHTPFRARAYVDTGPVQERVYAQYAGLGWIGKNTCLINPE
                     RGSWLFLGEIITSLALRPDPPGFDQCGTCTLCLEACPTGALTAPYVLDATRCLSYLTI
                     ELKQAIPATLREALGRHVYGCDICQDVCPWNQAAPVSRDPAWQPREGLDSPRLIDLWK
                     RRDAELHELVDGSAMTRPKLTGLRRNIAVAMGNSRAAGMAAALDASGGPDSPSLDDRV
                     VREHVAWARERTG"
     gene            complement(3147..>3348)
                     /locus_tag="PKK95_15710"
     CDS             complement(3147..>3348)
                     /locus_tag="PKK95_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19715.1"
                     /translation="LTGTEIYRVTGIADGLEPAGEVSVEARSPEGTRRFKAVVRIDTP
                     EELAAFQAGGILPYVLRQLVAG"
BASE COUNT          496 a         1178 c         1151 g          523 t
ORIGIN      
        1 aggaggcgtc ggtgccatta ttcgaatacg aatgccggaa gtgcggtcac catttcgagg
       61 cgctggtggt cgggtcgcgc cggcccgctt gcccgaagtg caagagcgaa gacctcgaga
      121 agaaggcgtc ggccttcggc gtggccgggg ccggggcctg gtcaggcgcg tcgagcggct
      181 gcgggacggg cggcggcggc ggttgacgct ggtagcgcag cgggttggtc caacccgagt
      241 acccatcgaa gaaccggctc actgtctgct gccgtgaatg ccgcggcggc agacagtgag
      301 acttcccccg ggcgacgctc tccttctcaa ttctccaaca gcctcctcgc cgactcgaag
      361 cgaggctcga tgtccaccgg gatccccgag aggcggtcga gcgccttctg cacctcgggc
      421 cgcaagacga ccaggcgctt cagcagcgcg tcggccttcc cgtaatcacc ctcggcctgc
      481 agggtcatca actccgaggt caggcgcgtc accgcgtcct tgattcgcgc gtggtcgacg
      541 gcgaaggtgc cgtcggggtt cacgttgaag gcgccggcat ccaggagcca gttcagctgc
      601 agcgcgatcc ccttcccgtg ggcctccgac agcccgaagc ggatcgagcg gaaggccgac
      661 gccaggaacg tctcgtacat cgtcggctgc agggccttgt cgaggacccc cttgtcgatg
      721 aggtactgaa gcgcgaaaag ccccgacacg tccgctttcg cctcctcgat ggcagagtag
      781 gtctccttga ggcgcgcgcg aacggtggtc tgctccccgc cggccgtgat ctggtgcggt
      841 ccgaggccgt gcatcagctc gtgcatcagg atgtgggtga agaacgcgtc gaaggttacc
      901 cgctgccggt cggcgggggc gagcacgcgt ccggaaatcg gcacgaggac gagcttgaac
      961 ttcgcctcct gcacgttgcg cagcatgacg cgcttggcgc ccttttcgcg gaccacccgc
     1021 tcgtcgttgg gcaggttgaa agctgccgtc tgcacgcccc ggtttccgtc gcctgcggcg
     1081 agcacgacgt tgacgacgcg gatcggcgcc agcgaaccga gcttcgggtt gcggaaggcg
     1141 ggggcgatcg ggaggtggtt ttcgatgtcc tgcagctcgc cgcccagcct ggccagcttc
     1201 gcgctctccg cctcgtcgcg cagggtgatg aaggcctcga acgcggcctt ggcgttgaac
     1261 cactcgtcct cgtagacctc gtaggggccg atggtcggct cgatcgaggc gtccagctcc
     1321 atccacgcca cgtcgctctc gtagtagtcg ttgctgagga acgcgtccgc gcgcgtttcg
     1381 aggaatcgct tcagcgtggg ctgcgcggtg ctggcggcgg cctcgcgcaa cagggccgcg
     1441 gcccgcgcca gctcgccctg gtactcgacg ctgtagggca ccgcctggaa cccgccgccg
     1501 ggcgcccggc ggatggtcgt gaagaacccg gccgcctccc cgcgggcctc gccggacagc
     1561 cgctgcatcc aggtctccac ctcggccttc gtcgaacccg cgggatagaa gttggcccct
     1621 tccggtttgg gcggcacgcc cgggacgaag ggacggttgt ggtcgagccg gtcccatgga
     1681 cccttgttga tcaggaagta gcggaggcgg gccttgccga gcgccgtggg gtcggcgagc
     1741 agctgctgga gcagcgccgg gttgccggcc cacacctggc ggaggaagag cccatccatc
     1801 acccaccccg cctccaccag cctggccaac gcctgccgct cgctggcggg aagggccgag
     1861 acgtcggcgc cgatctcgac gggggcaaac tgccgggcca acgcgtcgag cgccgcttga
     1921 tcgggcacgt tcacctcctg aggggtcacc ggggcgccag ccgcgacgac gaccgcggcc
     1981 accgccagga cgactgcgca tgtcgaagat ctcatgatgt tcctcgacct cgattctact
     2041 gaatgcccga gtccctcagc cggtccgctc ccgggcccag gcgacgtgct cgcgcactac
     2101 ccggtcgtcg agcgatggtg agtcgggccc gccgctcgcg tcgagggcgg cggccatccc
     2161 tgccgccctg ctgttcccca ttgccacggc gatgttgcgg cgcaggccgg tgagctttgg
     2221 cctcgtcatc gccgacccgt ccaccagctc gtgcagctcg gcgtcgcggc gcttccagag
     2281 atcgatcagg cggggcgagt cgagcccctc ccgcggctgc cacgcggggt cgcgcgagac
     2341 cggagccgcc tggttccacg ggcagacgtc ctggcagatg tcgcagccgt acacgtgccg
     2401 cccgagcgcc tcgcgcaggg tcgccggaat cgcctgcttc aactcgatcg tcaggtagga
     2461 gagacaccgt gtggcgtcga gcacgtaggg cgcggtgagg gcaccggtcg ggcacgcctc
     2521 caggcacagc gtgcacgtgc cgcactggtc gaagccggga gggtcgggac gaagcgcgag
     2581 gctcgtgatg atctcgccca ggaacagcca ggaaccgcgc tccgggttga tcaggcaggt
     2641 gttcttcccg atccacccga ggccggcgta ctgcgcgtac acccgctcct gcacgggccc
     2701 ggtgtccacg tacgcgcgcg cccggaaagg cgtgtgcgcc tggtcgcgca tccaggccag
     2761 caacgcctcg agccgccggc cgatcacgtc atgatagtcg tcgccccagg cgtaccgcga
     2821 gatgatggcc tcttcctcgt cggcggactc gacagagagc ggccggtcgg cgtgatagag
     2881 ggtggccgtc gcaatcaccg atcgggccga cggcagcacc tgccgcacgt cctcccgcac
     2941 gcgggcggtc cgcggcaggt agcccatggt gccggcgtaa ccgcgggcga gccactcgcg
     3001 gaagaacccc agctcgggcc aggcgccggc cggggcgatc ccacagaggt cgaacccgat
     3061 cgcccgcgcc ttctccttga tccgttcggc ggtcagcacg attcaccgta gggcggcctg
     3121 ccgccgcccc agctcttccc gctctcctac cctgcgacca gctgcctgag cacgtatggc
     3181 aggatcccgc ccgcctggaa cgcagccagc tcctccggcg tgtcgatgcg aaccactgcc
     3241 ttgaaccggc gcgtgccctc gggagaccgc gcctccaccg acacctcccc ggccggttcg
     3301 agcccgtcgg caatcccggt cacgcggtag atctcggtgc cagtgagc
//
LOCUS       DAOPQX010000398         3329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_31135_length_3329_cov_4.751374, whole genome shotgun sequence.
ACCESSION   DAOPQX010000398 DAOPQX010000000
VERSION     DAOPQX010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31135_length_3329_cov_4.751374"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..425
                     /locus_tag="PKK95_15715"
     CDS             195..425
                     /locus_tag="PKK95_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19716.1"
                     /translation="MLSTIVASLVVFSVAWRQAAGNLAGCVADRQGAALCGPHFVFRL
                     TGELALGFGEGHTLTREGLLSQLRALADRRAR"
     gene            572..1744
                     /locus_tag="PKK95_15720"
     CDS             572..1744
                     /locus_tag="PKK95_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOC19717.1"
                     /translation="MSTPPVDRDFIVSTLAGLVRLDSINPSIAPEGAGEAAIARSLAR
                     TFESLGLDVEILEGQAGRPSVLGTLRGTGGGPSLMFNGHEDTVGVEGMPGPFSAEIRG
                     KRMYGRGTHDMKGSLAACAGAVKALVDGRVRLRGDVVVAAVADEEYGSLGTREVLAHR
                     TVDAAIVTEPTNLAICLAHKGYVWIEVETKGRAAHGSRFTEGIDANLRMGRFLARLEG
                     LERSLRTGPSHPLVGPPSLHAALLRGGSGMSTYAERCRLTIERRTVPGETERSVFEPL
                     MAAAAGLHSEDPSFVASVRSVFARNPWEVSPDAAIVRVLADAATGVLGERPAFIGDTP
                     WMDAALLAEAGVETVVMGPTGAGEHSAVEWVDLDSVVTFAELLATAAILYCGTEQG"
     gene            1825..2289
                     /locus_tag="PKK95_15725"
     CDS             1825..2289
                     /locus_tag="PKK95_15725"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19718.1"
                     /translation="MNPARRQAAILDLVDRERIDSQRVLRQRLRARGIVATQTTISRD
                     LKALGLVKRAADGAYARPGQEGGAPIEARLAQLSRVLGRSVARATRVQQLVVLRTPPG
                     EAQQVAVAIDRAALDEIVGTIGGDDTVLIIAGSSRQARAFLGRLEALVAGGK"
     gene            complement(2316..2801)
                     /locus_tag="PKK95_15730"
     CDS             complement(2316..2801)
                     /locus_tag="PKK95_15730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOC19719.1"
                     /translation="MLALPSPRPARIAIIGVGNQLNGDDGAGPHVVRELAARLPATPG
                     LLLLDGGLAPENFSGPLRRFRPDLVLQIDAADQGRPPGATAWIDWQEADGLSASTHTL
                     PPSVFAKFLIAELGCRVALIGIQPAVLELNAPLSPAVDRACSELADALGKWLTAAEVP
                     R"
     gene            complement(2801..3220)
                     /locus_tag="PKK95_15735"
     CDS             complement(2801..3220)
                     /locus_tag="PKK95_15735"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOC19720.1"
                     /translation="MRIGAMLGDVSRALFKRPATELYPFERREAPGRLRGLLVFEPSR
                     CTGCKICMRDCPARALELIVVDKATKRFAMKFHADRCTYCAQCVVSCNFDSLSMSHEQ
                     WELAGLAREQFLHTFGRDEDLAVLREREAAAPPGQGV"
BASE COUNT          513 a         1109 c         1219 g          488 t
ORIGIN      
        1 ggcctgcacc tgctgaacgg cagcctcggc tgacgcttga accacaaagg acacagagaa
       61 cacaaaggtc gccaggattg atcgcactcg tcgcggtgcc cccgctaccc aattccacga
      121 cacgtctcaa ggcgcttgcg gcgataatgc cggcaggcaa cctggtcagc cggacgaacg
      181 cctggaggtg acgcatgctg agcaccatcg tggcgtcgct cgtggtcttc tcggtcgcgt
      241 ggcgccaggc cgctggcaac ctggccgggt gcgttgctga caggcaaggc gccgccctgt
      301 gcggaccgca ctttgtgttc cggctgacgg gtgaactcgc cttgggcttc ggtgagggac
      361 acaccctgac acgcgaaggt cttctctcgc aactccgcgc cctggctgac cgccgggcgc
      421 gttgacgttc gatgatcccc cgcaccacag cgccgtccaa ctcggcacct cttcgtgatc
      481 tttgtgatct ttgtggtcac atcgtacgtg ggcatacgag caggcgatgg atgctgctgg
      541 tagaatgagc cggccgcaag gttgttcttc catgtcaacc cctcccgtcg accgtgactt
      601 catcgtctct acgctcgccg gcctggtgcg cctcgactcg atcaacccgt cgattgcccc
      661 ggagggtgcc ggtgaggccg ccatcgcgcg aagcctcgcg cggaccttcg aatccctggg
      721 cctcgacgtc gagatcctcg agggccaggc gggccgcccc tcggtgctgg gcacgctgcg
      781 cggcacggga ggcgggccct cgctgatgtt caacggccac gaggacacgg tcggcgtcga
      841 ggggatgccg gggccgttct ccgccgagat ccgcggcaag aggatgtacg gccgcggcac
      901 ccacgacatg aagggaagcc tggcggcgtg cgcgggcgcg gtcaaggcgc tggtggacgg
      961 gcgggtgcgc ctgcggggcg acgtcgtggt ggcggcggtc gccgacgagg agtacggcag
     1021 cctgggcacc agggaggtgc tcgcgcaccg cacggtggat gcggcgatcg tcaccgagcc
     1081 cacgaacctc gccatctgcc tcgcgcacaa gggatacgtc tggatcgagg tggagacgaa
     1141 ggggcgggcc gcgcacggca gtcgcttcac cgagggaatc gacgcgaacc tccgcatggg
     1201 gcggtttctc gcccggctcg agggcctgga gcgatcgctg cgcaccggtc cgtcgcaccc
     1261 gctcgttggg ccgccctcgc tgcacgccgc gctgctgcgc ggcggaagcg gcatgagcac
     1321 ctacgcggag cggtgccggc tcacgatcga gcggcgcacc gttccgggtg agaccgagcg
     1381 gtcggttttc gagccgctga tggccgccgc cgcgggcctg cactcggaag acccgtcgtt
     1441 cgtcgcgtcg gtgcgaagcg tgttcgcgcg caacccctgg gaggtgtcgc cggacgcggc
     1501 catcgtgcgc gtcctcgccg acgcagccac gggagtgctc ggcgagcgcc ccgccttcat
     1561 cggcgacacg ccgtggatgg acgccgcgct gctggcagag gccggggtcg agacggtggt
     1621 catggggccg accggcgcgg gcgagcactc ggccgtggaa tgggttgacc tcgacagcgt
     1681 ggtcaccttc gcggaactcc tggcgacggc ggcgattctc tattgcggaa cggagcaggg
     1741 ctgaagccct gctctacgag gagcactgaa agcagggcca aagccctgtc tacgggaaga
     1801 tatggaagat acgggagagt ggtcatgaac ccggcacggc ggcaggccgc catcctcgat
     1861 ctcgtcgacc gggagcggat cgacagccag cgcgtcctgc gacagcgcct gcgggcgcgc
     1921 ggcatcgtcg cgacccagac aacgatttca cgcgatctga aggcgctcgg gctcgtgaag
     1981 cgcgccgccg acggggccta cgcccgcccg gggcaggagg gaggagcgcc gatcgaggcg
     2041 cggctggccc agctctcgcg agttctcggc cgctcggtgg cccgcgccac ccgcgtccag
     2101 cagctcgtcg tgctgcggac gccgcccggg gaggcgcagc aggtggcggt ggcgatcgac
     2161 cgggcggcac tcgacgagat cgtcggcacc atcgggggtg acgacaccgt cctgatcatt
     2221 gcgggcagct cgaggcaggc cagggccttc cttggccggc tcgaggccct ggtcgcggga
     2281 gggaagtagg gggcaagggg cagcggcttc ccgcttcagc gcggaacctc ggctgcagtc
     2341 agccacttcc cgagcgcgtc ggccagctcg ctgcaggcgc ggtcgacggc cggggagagc
     2401 ggcgcgttca gttcgaggac ggcaggctgg atcccgatga gcgcgacccg gcagcccagc
     2461 tccgcgatga ggaacttcgc gaagaccgag ggcgggaggg tgtgggtgga ggcgctgagg
     2521 ccgtcggcct cctgccagtc gatccaggcg gtggcgcccg gcgggcggcc ctggtcggcg
     2581 gcgtcgatct gaaggacgag gtcgggtcgg aatcgccgga ggggaccgct gaagttctcg
     2641 ggggcgaggc cgccgtcgag caggagcagg ccgggagtgg ccggcaggcg cgcggccagc
     2701 tccctcacaa cgtgcgggcc cgctccatcg tcgccgttca gctggttgcc gacgccgatg
     2761 atcgcgattc gggcggggcg aggagaggga agagccagca tcacaccccc tggccaggcg
     2821 gcgccgcggc ctcgcgctcg cgcagcacgg cgaggtcctc gtcgcgcccg aaggtgtgga
     2881 ggaactgctc gcgcgccagg ccagccagct cccactgttc gtgcgacatc gagaggctgt
     2941 cgaagttgca gctcaccacg cactgggcgc agtaggtgca ccggtcggcg tggaacttca
     3001 tcgcgaaccg cttggtggcc ttgtccacca cgatcagctc gagcgcccgg gcggggcagt
     3061 cgcgcatgca gatcttgcag ccggtgcagc gtgacggctc gaagacgagc aggccgcgca
     3121 gccggccagg ggcctcccgg cgttcgaagg ggtagagttc ggtcgcggga cgcttgaaca
     3181 gcgcccgcga tacatccccg agcatggcac cgattcgcat ggtgaacctc gaaatctccg
     3241 gggggcctcc gccccgcgtc aaccactcac agccttcacc gccgtgagga gtgaaatcga
     3301 tccgctccgc tagcccctag ctcctagcc
//
LOCUS       DAOPQX010000399         3326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_31167_length_3326_cov_3.930297, whole genome shotgun sequence.
ACCESSION   DAOPQX010000399 DAOPQX010000000
VERSION     DAOPQX010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31167_length_3326_cov_3.930297"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKK95_15740"
     CDS             <1..546
                     /locus_tag="PKK95_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOC19721.1"
                     /translation="DQRFASQRPDVLVYQTEPLAADLTVAGPIRPRLQVSTSGTDSDF
                     VVKVIDVYPDGYPDPDPAPGPPSPFASPYTRMGGYQQLVRGEVFRGKFRRSFERPEPF
                     EPNQVATIEYVMPDVFHTFRRGHRIMIQVQSTWFPLVDRNPQTFVTIPEATEADFQKA
                     TQRVYRNAAHPSRVELTVWER"
     gene            complement(605..2035)
                     /locus_tag="PKK95_15745"
     CDS             complement(605..2035)
                     /locus_tag="PKK95_15745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC19722.1"
                     /translation="MPAKVRHGLAGPEKMDASAPAVSGPGSSVEYPPWWEAWAIRARA
                     RLAGRWKNNRVAREAAALALVARAHLPGRTTPQRLTDLITACKHAQSRRLRRLLEARI
                     APFLSGPRAGIWRAEKIGWSRFYGEFGDLRAQKALTTSLLLKAPGPGGEKGVLYSAFE
                     YNWMRLIAHHDARAFLSEYTLVGASSYSPTHYAALANFAGLSDDPVFIGVSNHADLEA
                     YRVLSPVIEPVPLLACDLNDPNQYTPKPHAERRIDILMVATWLRVKRHWLLWEALAGM
                     PRDLRVVLVGRNLPGRTAHDLLREARAFGVRQHLEFELNVPPERVAELQCDARVSAVF
                     SDREGSCVAVTESMFADSPVAMMADAHVGAKAYINEQTGVLLRRKGIGAQLRRFLETS
                     HLFTPRAWALRNVSCHVSARRLNDILRADALARGRPWTEDIAPFCRRYVAQYIDASDE
                     ARLAPAVAELERRHGVRLTRWEYRAT"
     gene            complement(1998..3152)
                     /gene="speY"
                     /locus_tag="PKK95_15750"
     CDS             complement(1998..3152)
                     /gene="speY"
                     /locus_tag="PKK95_15750"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOC19723.1"
                     /translation="MSILDPHRKMSHPPLEGGVEVTRLVDTYFNAYNGARLREACQLF
                     VKLIEEDATVGVTLAGALTPAGLGSVLVPLIRAGFVDYIASTGANLYHDLHFTLGYPL
                     YRSTAQVASGAADVELRKKGIIRIYDVLFDQKVLLETDDWLYRTLLEDEFRKTMGTSE
                     LHHRVGAHALEAARTRGIAPPVLATCHECDVPIYAPSPGDSTVGMNVSAVWTARPDLA
                     PVVNPAIDVAETAAIVLEAKRGDRPSAVVIFGGGAPKNFLLQTEPQLQEVLGIDERGH
                     DYFIQVTDARPDTGGLSGATPSEAVSWGKIDPDQLPNTVVCYADSTIAMPLIAAYALS
                     KAKPRPLRRLYQRMPELVERLREEHRGTSLYRERWGGQDAGEGPPRPGRA"
BASE COUNT          491 a         1145 c         1165 g          525 t
ORIGIN      
        1 gaccagcgct tcgcgtcgca gcggcccgac gtgctcgtct accagaccga gccgctcgcg
       61 gccgacctca cggtggccgg tcccatcagg ccgcgcctcc aggtgtcgac gagcggcacc
      121 gactcggatt tcgtggtgaa ggtgatcgac gtctatcccg acggctatcc cgatcccgac
      181 cccgcgccgg ggccgccgag cccgttcgcc tcgccgtaca cccggatggg cggctaccag
      241 caactggtga ggggcgaggt gttccggggc aagttccgcc gcagcttcga gcgtcccgag
      301 ccgttcgagc cgaaccaggt ggccacgatc gagtacgtga tgcccgacgt gttccacacc
      361 ttccggcgcg gccaccggat catgatccag gtccagagca cctggtttcc gcttgtcgat
      421 cgcaacccgc agaccttcgt gacgatcccg gaggcaaccg aggccgactt ccagaaggcg
      481 acccagcgcg tgtaccggaa cgccgcccac ccgagccgcg ttgagctgac ggtgtgggag
      541 aggtgacctt ttggtggccg caggcctgaa ggcctgcgct acggaggcgc atcggtgact
      601 gaagctaggt ggctctgtac tcccaccgcg tgaggcgtac gccgtggcgg cgttcgagct
      661 cggcgacggc cggagccagc ctggcctcgt ccgacgcgtc gatgtactgc gccacgtagc
      721 gccggcagaa gggggcgatg tcctcggtcc aggggcgccc gcgcgcgagg gcgtcggcgc
      781 ggaggatgtc gttcaggcgc cgggccgaca cgtggcagct gacgttgcgg agcgcccacg
      841 cccgcggcgt gaagaggtga ctggtctcga ggaaccggcg cagctgcgcg ccgatgccct
      901 tcctgcgcag cagcaccccc gtctgctcgt tgatgtaggc cttcgcgccg acgtgggcgt
      961 cggccatcat cgccaccggc gagtcggcga acatgctctc ggtgaccgcg acgcacgacc
     1021 cctcgcggtc gctgaacacg gccgacaccc gcgcatcgca ctgcagctcg gccacccgct
     1081 ctggcggcac gttcagctcg aactcgaggt gctggcgcac gccgaacgcc cgggcctcgc
     1141 gcagcaggtc gtgcgcggtc cggccgggca ggttccgccc cacgagaacc acgcgcaggt
     1201 cgcgcggcat gccggcaagc gcctcccaca gcagccagtg gcgtttcacg cggagccagg
     1261 tcgccaccat caggatgtcg atgcggcgct cggcgtgcgg cttgggcgtg tactgattgg
     1321 ggtcattcag gtcgcacgcc agcagcggca ccggctcgat gacggggctg agcacccggt
     1381 aggcctcgag gtcggcgtgg ttcgacaccc cgatgaagac cgggtcgtcc gagaggccgg
     1441 caaagttggc gagggccgcg tagtgggtgg gggagtagga cgaggcgccg accagggtgt
     1501 actcggagag gaaggctcga gcgtcgtgat gcgcgatgag gcgcatccag ttgtactcga
     1561 aggccgagta gagcaccccc ttctcgccgc ccggcccggg cgccttcaag agcaggctcg
     1621 tcgtcagcgc cttctgggcc ctgaggtcgc cgaactcgcc gtagaagcgg ctccagccaa
     1681 tcttctccgc ccgccagatc ccggcgcgcg gtcccgacag gaacggcgcg atccgggcct
     1741 cgagcaggcg ccggaggcgc cggctctgcg cgtgcttgca ggcggtgatg aggtcggtga
     1801 gccgctgcgg cgttgtccgg ccgggcaggt gggcacgggc gaccagcgcc agcgcggccg
     1861 cctcgcgcgc aacgcggttg ttcttccacc gccccgcgag gcgtgcgcgg gcccggatgg
     1921 cccaggcttc ccaccagggc gggtactcga ccgacgatcc cggcccggac accgcggggg
     1981 cggaggcgtc catcttctca ggccctgcca ggccgtggcg gaccttcgcc ggcatcctgc
     2041 ccgccccacc gctcccggta gagcgaggtg ccacggtgct cctcccgcag ccgctcgacc
     2101 agctccggca tgcgctggta gagccgccgg agcggcctgg gcttcgcctt cgacagggcg
     2161 taggccgcga tcaggggcat cgcgatcgtg ctgtccgcgt agcagaccac cgtgttcggg
     2221 agctggtcgg ggtcgatctt gccccacgac accgcctcgg acggcgtcgc acccgagagt
     2281 ccgcccgtgt cggggcgcgc atcggtgacc tggatgaagt agtcgtgacc gcgctcgtcg
     2341 atccccagca cttcctgcag ctgcggctcg gtctggagca ggaagttctt cggcgccccg
     2401 cccccgaaga tgaccaccgc gctcggacgg tcgccgcgct tcgcctcgag gacgatggcc
     2461 gccgtttcgg cgacgtcgat cgcggggttc accacgggcg cgagatcggg ccgggcggtc
     2521 cacacggccg agacgttcat gcccaccgtc gagtcgcccg ggctgggcgc gtagatcggg
     2581 acgtcgcact cgtggcacgt cgcgagcacg gggggagcga tcccccgcgt ccgcgccgcc
     2641 tcgagcgcgt gtgctcccac gcggtggtgc agctcggatg tccccatcgt cttccggaac
     2701 tcgtcttcga gcagcgtcct gtagagccag tcgtcggtct cgagcaggac cttctggtcg
     2761 aacagcacgt cgtagatgcg gatgatcccc ttctttcgca gctcgacgtc ggccgccccg
     2821 ctcgccacct gggccgtcga tcgatagagc ggataaccca gggtgaagtg caggtcgtgg
     2881 tagaggttgg ctcccgtcga tgcgatgtag tcgacgaagc ccgccctgat gagcggcacc
     2941 agcacggacc ccagccctgc cggtgtcagt gcccctgcaa gggtgacgcc gaccgtggcg
     3001 tcctcctcga tcagcttcac gaagagctgg caggcctctc gaagccgcgc tccgttgtag
     3061 gcgttgaaat aggtgtcaac gaggcgcgtc acctcgacgc ccccttcgag cggcgggtgc
     3121 gacatcttgc ggtgaggatc gaggatgctc atggctccac tgtaacacct gaaagtcaca
     3181 ggatgagtag gggcaactct cgcgacttcg cgacttcgtg gacttctcga cttctggact
     3241 ggcggacgcc cggagtccgt cctgtgtatc attcggtcag ggaggaccga tgagagcatt
     3301 ctcggggatg ttgctggtgt gcttcg
//
LOCUS       DAOPQX010000400         3307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_31402_length_3307_cov_4.670972, whole genome shotgun sequence.
ACCESSION   DAOPQX010000400 DAOPQX010000000
VERSION     DAOPQX010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31402_length_3307_cov_4.670972"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PKK95_15755"
     CDS             complement(<1..783)
                     /locus_tag="PKK95_15755"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOC19724.1"
                     /translation="MFTISPELLSASTVAVRDCMGAQPGEKVLIVTDAPLRLIGYALH
                     KAATELGLEVLLVDMPPRKSNGEDPPPEVADLMARSDVVLCPTSKSLTHTDARRAASA
                     SGARIATLPGVTEVMMVRCMNADYHAIAARTHRLCERLERASVIRVKAPGGTDVTMPM
                     AGRRAHASTGLFHEKGQWGNLPTGEAYLAPLEGRSNGVVVVDGSMAGVGVVKTPIRIE
                     VNDGYATDISGGAEARLLTELLAPHGQDARTVAEFGIGTNDKA"
     gene            complement(792..1763)
                     /locus_tag="PKK95_15760"
     CDS             complement(792..1763)
                     /locus_tag="PKK95_15760"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOC19725.1"
                     /translation="MVPSFTYVRAKSVADLVRQLAAPGARVHAGGTDLMGCLRDGVFK
                     ADKLVSLSGLADLKGIAAATGGGLRIGALTTNSEVAANPVIVERYPGLAQAARAVASP
                     QLRNQGTIGGNLCQKPRCWYYRGEFHCLRKGGDMCYAVGGENQYHCIFGGDACFIVHP
                     SDTAPALTALEARVRVAGPGGSRVVPIGQFFVLPAKDPTRETVLERNEVVTEILLPPA
                     ETGLRSSYRKVRGRGAWDFALAGVALALLMRDGTIERARVVLSGAAPVPWRAQAAEKA
                     LVGKAITPETAAAAAEAAMQGAEPMAKNGYKVPMFKGAIVESLLALA"
     gene            complement(1767..>3307)
                     /locus_tag="PKK95_15765"
     CDS             complement(1767..>3307)
                     /locus_tag="PKK95_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOC19726.1"
                     /translation="NTFHHFGFAALILKGGDLTGSIAKKTGRPVKYHLSREEAFLAVG
                     NRPANQMTVKAGAKKDGTLTALEMTGTGSSGAYPGGGVGGVDFVVRDLYRCPNVRTEL
                     QDVYINAGQVRAFRAPGHPQGAWALEQTLDELAEKLGLDPVEFRVRNVPEVSQSRGNT
                     PYSTTGLRACLEEGAKRFGWAEARKRAQAQGVIRRGVGVAAGMWQGGAGSPPATIVVK
                     VFADGSANLNMGAADIGTGTKTVMAMVVSEELGIPLDRITVEWADTGTTQFATPSGGS
                     KTVPTESPAVRAAALDVKQQLLQMAAEQLKLAPEEVAFRGGEIVAVADPSKKVAVGDL
                     QGLRRRGLAVGVGYRGPNPAGMVTCPFAAHFAEVEVNTGTGEVKVTRFLAAQDSGRPL
                     NELTYENQVHGGLVMGLGLAMMEARILDGQQTGKMVNRNWHDYKVPTAMDLPADQAVL
                     PIDLHDYKANSTAVKGIGEPATIPTAPAIANAIYHACGVRTPDAPVNPARLVSLLSAP
                     NRKG"
BASE COUNT          426 a         1241 c         1114 g          526 t
ORIGIN      
        1 cgccttgtcg ttggtgccga tgccgaactc ggcgaccgtg cgcgcatcct gcccgtgcgg
       61 ggccagcagc tcggtgagca ggcgggcctc ggcgccgccg ctgatgtcgg tcgcgtagcc
      121 gtcgttcacc tcgatgcgga tgggggtttt cacgaccccc acgcccgcca tggagccgtc
      181 caccaccacg accccgttcg atcgcccctc gagtggcgca aggtacgcct ctccggtggg
      241 caggttgccc cactggccct tctcgtggaa cagccccgtg ctcgcgtggg cccgcctgcc
      301 ggccatcggc atggtgacgt cggtcccgcc cggggccttg acccggatga ccgaggcacg
      361 ctcgagccgc tcgcacaggc ggtgcgtgcg ggccgcgatc gcgtggtagt cggcgttcat
      421 gcaccgcacc atcatgacct cggtcacacc gggcagcgtc gcgatgcgcg cgcccgacgc
      481 gctggcggcg cgccgcgcgt cggtgtgcgt cagcgacttc gacgtcgggc agagcacgac
      541 atccgagcgg gccatcaggt cggcgacctc ggggggcgga tcttcgccgt tgctcttgcg
      601 gggcggcatg tccaccagca acacctcgag gccgagctcg gtggccgcct tgtggagcgc
      661 gtagccgatc agcctgaggg gcgcgtcggt gacgatgagc accttctcgc cgggctgcgc
      721 acccatgcaa tcgcgaaccg ccaccgtgga ggccgacaac aactctggtg agatggtgaa
      781 catgctttgc ctcacgccag cgcgagaagc gactcgacga tcgcgccttt gaacatcggg
      841 accttgtagc cgttcttcgc catcggctcg gcgccctgca tcgccgcctc cgcggccgcg
      901 gccgcggtct cgggcgtgat ggccttcccc accagcgcct tctcggcggc ctgcgcccgc
      961 cacggcaccg gcgccgcgcc cgacagcacg acgcgcgcgc gctcgatggt gccatcccgc
     1021 atcagcagcg cgagcgccac gccggcgagc gcgaagtccc aggccccccg cccccgcacc
     1081 ttccggtacg agctgcgcag cccggtctcg gcaggcggca gcaggatctc ggtcacgacc
     1141 tcgttccgct cgagcaccgt ctcacgggtc gggtccttcg cggggagcac gaagaactgg
     1201 ccgatcggaa cgacgcggct cccgcccggg ccggccaccc gcacgcgcgc ctccagcgcc
     1261 gtcagcgcgg gggccgtgtc ggagggatgg acgatgaagc aggcgtcgcc gccgaagatg
     1321 cagtggtact ggttctcacc gcccaccgcg tagcacatgt cgccgccctt acgcaggcag
     1381 tggaactctc ctcggtagta ccagcaccgc ggcttctggc agaggttccc gccgatcgtc
     1441 ccctggttgc gcagctgggg gctggcgacc gcccgcgccg cctgggccag ccccgggtag
     1501 cgctccacga tcacggggtt cgccgccacc tcgctgttgg tggtcagcgc cccaatccgg
     1561 agcccgccgc ccgtcgcggc ggcaatcccc ttcaggtcgg cgagcccgga gaggctcacc
     1621 agcttgtcgg ccttgaagac gccgtcgcgc aggcacccca tcaggtcggt gccgcccgcg
     1681 tgcacccggg ccccgggtgc cgcaagctgc cggacgaggt ccgctaccga tttcgcccgc
     1741 acgtacgtaa agctcggcac catggcttat cccttcctgt tgggcgcgct cagcagcgac
     1801 acgagccgcg cgggattgac cggcgcgtcg ggggtcctga cgccgcaggc atggtagatc
     1861 gcgttggcga tcgccggcgc ggtcgggatc gtggccggct cgccaattcc cttcactgcc
     1921 gtgctgttcg ccttgtagtc gtgcaggtcg atgggcagca cggcctggtc ggccggaagg
     1981 tccatggcgg tcggcacctt gtagtcgtgc cagttccgat tgaccatctt gcccgtctgc
     2041 tgcccgtcga ggatccgcgc ttccatcatg gccaggccca gccccatgac gaggccgccg
     2101 tgcacctggt tctcgtaggt cagctcgttg agcggccggc cgctgtcctg cgccgcgagg
     2161 aaccgcgtca ccttcacctc gccggtcccg gtattgacct cgacctcggc gaagtgcgcg
     2221 gcgaacgggc aggtgaccat gcccgcgggg ttcggcccgc ggtagcccac tccgacggcc
     2281 agcccgcgcc gccgcaaccc ctggaggtcg ccgacggcga ctttcttgga ggggtctgcc
     2341 acggccacga tctcgccgcc ccggaacgcg acctcctccg gcgcgagctt cagctgctcg
     2401 gccgccatct gcagcagctg ctgcttgacg tccagggccg ccgcgcgcac cgcgggcgac
     2461 tcggtcggca ccgtcttgct gccgccgctc ggtgtggcga actgcgtcgt cccggtgtcg
     2521 gcccactcca ccgtgatccg gtcgagcgga atccccagct cctcgctcac caccatcgcc
     2581 atgacggtct tggtgccggt gccgatgtct gcggcgccca tgttcaggtt ggcgctccca
     2641 tcggcgaaca ccttgacgac gatggtggcc ggagggctgc ccgcgccgcc ctgccacatc
     2701 cccgcggcca cgccaacccc ccgccggatc acaccctggg cctgggcgcg cttgcgcgcc
     2761 tcggcccatc cgaaccgctt cgccccctcc tcgagacagg cccgaagtcc ggttgtggag
     2821 tacggcgtgt tgccgcgcga ctggctcacc tcggggacgt tcctcacccg gaactcgacc
     2881 gggtcgaggc caagcttctc ggccagctcg tcgagcgtct gctcgagcgc ccaggccccc
     2941 tgcgggtgcc cgggggcccg gaaggcgcgc acctggccgg cgttgatgta cacgtcctgc
     3001 agctcggtcc tgacgttggg gcaccggtag aggtcgcgca cgacgaagtc gacgccgccc
     3061 acaccgccgc ccgggtaggc gccgctgctg ccggtaccgg tcatctcgag cgcggtgagc
     3121 gtcccgtcct tcttcgcccc ggccttcacc gtcatctggt tggcgggccg gttgccgacg
     3181 gcgaggaagg cctcctcgcg cgacaggtgg tacttgaccg ggcggcccgt cttcttcgcg
     3241 atcgagccgg tcaggtcgcc gcccttcagg atgagcgcgg cgaaaccgaa gtggtggaac
     3301 gtgttgt
//
LOCUS       DAOPQX010000401         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_31594_length_3291_cov_4.694994, whole genome shotgun sequence.
ACCESSION   DAOPQX010000401 DAOPQX010000000
VERSION     DAOPQX010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31594_length_3291_cov_4.694994"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PKK95_15770"
     CDS             complement(<1..1143)
                     /locus_tag="PKK95_15770"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC19727.1"
                     /translation="MTFVHDLRHATRVLARNPGVSLIAIFTLALAIGATTAVFSVVQG
                     VLLRPLPYPDPDRLAAIWEVNDRGTFSRLADPNFNDFRDRNRTFAVMAKYAGMIESVT
                     GTGEPTREMVATVTRDFFTVLGVQPALGRPFAPDDCRLGAVPTVVVSHRYWARSLGAG
                     ASLSAFRLSIANRVHEVIGVMPAGFEFPARADLWVPAELDEENTSRTSHNYWAVGRLR
                     DGVDVARASAELNTIAADIIRHSPEQGRYLMTGAAAEPLHASLTGRAGQTLYLLLGAV
                     FFLLLVACANVTNLLLAQAAARQREIAVRRALGAGRAGLVQQFVAEALVLLAAGALLG
                     ILAASAGTSTLISIAPADLPRLNEVSMNGVVLAFAVGLSALVAVGLG"
     gene            complement(1220..1879)
                     /locus_tag="PKK95_15775"
     CDS             complement(1220..1879)
                     /locus_tag="PKK95_15775"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOC19728.1"
                     /translation="MPDPKELTAYVDQLAAVLTYLAVVTSAISGALEARHRHMDIVGA
                     ITVAFVTAFGGGTLRDLLLGRTPIFWVLDPWLTVATFVPAVASYYAVKRVSDRLVLVP
                     DALGLGFFSTLGATYAMQMNLPTLVTVLMGVITGVFGGVMRDTICNRVPLVFRRNTEL
                     YATCSFIGVWVFVLLMHVTPGAAFASWVGTAVVVVLRLISVRFRIRLPNPAPSGPFRT
                     N"
     gene            complement(1895..2788)
                     /locus_tag="PKK95_15780"
     CDS             complement(1895..2788)
                     /locus_tag="PKK95_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOC19729.1"
                     /translation="MEREALYGGIEGGGTKFVCAAGTGPDDIRAETRFPTTTPAETIS
                     RAIEFFRQNPVASLGVACFGPLDPDPCSPTYGRILATPKPGWADVDVAGPLGAALSVR
                     VAFDTDVNGAALAEWRWGAAKDCDPVVYVTIGTGIGGGLLTRGGLVHGLLHPEMGHVR
                     IPHDTVRDSFPGACPFHGDCFEGLASGVAIERRWGRKAETLPADHPAWELEARYVGLA
                     LANYVCTLAPQKIVVGGGVAQQPQVLPLVRRHVRDILGGYIRSPHVAGRIDEYIVPPA
                     LGGRAGVLGAIALARTIAAAR"
     gene            complement(3036..>3291)
                     /locus_tag="PKK95_15785"
     CDS             complement(3036..>3291)
                     /locus_tag="PKK95_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOC19730.1"
                     /translation="KGSLVLTAEDRPGALADALGVFAARGLNLSRIESRPTRIRPGTY
                     AFLIDFIGRPGDPEVESALASLRSQCLSLKVLGFYPAASR"
BASE COUNT          518 a         1151 c         1214 g          408 t
ORIGIN      
        1 gcccaggccc acggcgacca gcgccgagag gccgacggcg aaggccagca ccaccccgtt
       61 catcgagacc tcgttcaacc ggggcagatc ggcgggcgcg atcgagatga gcgtgctcgt
      121 cccggccgag gcggccagga tgccgagcag ggcgccggcg gccagcagaa ccagcgcctc
      181 ggcgacgaac tgctgcacca ggccggcccg cccggcgccg agcgcgcggc gcacggcgat
      241 ctcgcgctgc ctggccgcgg cctgggcgag caggaggttc gtgacgttgg cgcaggcgac
      301 gagcaggagg aagaagacgg ccccaagcag caggtacagc gtctggccgg cgcgtcccgt
      361 cagcgaggcg tggagcggct cggcggcggc gccggtcatc aggtagcgtc cctgctcggg
      421 ggaatgccgg atgatgtcgg cggcaatcgt gttcagctcg gcactggccc gggccacgtc
      481 caccccgtcg cgaaggcggc cgacggccca gtagttgtgc gaggtgcggc tggtgttctc
      541 ctcgtccagc tcggccggta cccagaggtc ggcacgggcg gggaactcga agccggccgg
      601 catcacgccg atcacctcgt gcacccggtt cgcgatgctc aggcggaacg cggagagcga
      661 cgcccccgcg ccgagcgatc gcgcccagta cctgtggctg accaccaccg tcggcaccgc
      721 gccgaggcgg cagtcgtcgg gggcgaacgg gcggccgagc gcgggctgca cgcccagcac
      781 ggtgaagaag tcgcgggtca ccgtcgcgac catctcgcgc gtcggctcgc cggtcccggt
      841 caccgactcg atcatgcccg cgtacttcgc catcacggcg aaggtgcggt tgcgatcgcg
      901 gaagtcgttg aagttcgggt cggcgaggcg cgagaaggtc ccgcggtcgt tcacctccca
      961 gatggccgcg agccgatccg ggtcggggta gggcagcggg cgcagcagca cgccctgcac
     1021 gacgctgaag acagcggtcg tggcgccgat ggccagcgcg agcgtgaaga tggcgatgag
     1081 gctgacgccg gggttcctcg cgagcacccg ggtcgcgtgg cggagatcgt gcacgaaggt
     1141 catcagggct ctcccctggc caattggacg aggggaacgg ccgagaggtt tccctgaacc
     1201 ggcgtaaaac ggtgcgccat cagttcgtgc ggaaggggcc gcttggagcc gggttcggca
     1261 gccggatgcg gaaccggacc gagatcaggc gcaggacgac gacgaccgcc gtgcccaccc
     1321 aggaggcgaa ggccgcaccc ggcgtgacgt gcatcaggag cacgaacacc cacacgccga
     1381 tgaacgagca ggtggcgtac agttccgtgt tgcggcggaa caccagcggc acccggttgc
     1441 agatcgtgtc ccgcatcact ccgccgaaga ctcccgtgat cacccccatc agcaccgtga
     1501 cgagggtcgg caggttcatc tgcatggcgt aggtcgcgcc gagcgtgctg aagaacccca
     1561 ggccgagcgc gtcgggaacg agcaccaggc ggtcggacac ccgcttcacc gcgtaatacg
     1621 acgccacggc cggcacgaag gtggccaccg tcagccacgg atcgagcacc cagaagatgg
     1681 gcgtccggcc gagcagcagg tcgcgcaacg ttccgccgcc gaacgcggtc acgaacgcca
     1741 ccgtgatggc gccgacgatg tccatgtggc ggtggcgcgc ctcgagggcg cccgagatcg
     1801 ccgaggtgac gaccgcgagg tacgtgagca ccgcggccag ctggtcgacg taggcggtga
     1861 gctcctttgg atcgggcacg gcgggtcgtt cgcctcatcg cgcggctgcg atcgtccggg
     1921 ccagcgcgat ggccccgagg acgccggccc gtccccccag tgccgggggc acgatgtact
     1981 cgtcgatgcg cccggcgacg tgcggcgacc ggatgtagcc gcccaggatg tcgcgcacgt
     2041 gacgccggac gagcggcagc acctgcggct gctgtgccac gcccccaccg acgacaatct
     2101 tctgcggggc gagcgtgcag acgtagttcg ccagcgccag gcccacgtac cgggcctcga
     2161 gctcccaggc gggatgatcc gccggcaggg tctcggcctt gcgcccccag cgccgctcga
     2221 tcgcgacgcc cgaggccagg ccctcgaagc agtcgccgtg gaacgggcag gcgcccggaa
     2281 acgagtcgcg gaccgtgtcg tgcgggatcc ggacgtgtcc catctcggga tgcagcaggc
     2341 cgtggacgag gcctccgcgg gtcaacaggc cgccgccgat ccccgtgccg atcgtcacgt
     2401 agacgaccgg gtcgcagtcc ttcgccgcac cccaacgcca ctcggcaagg gccgcgccgt
     2461 tcacgtcggt gtcgaaggcc accctcacgc tgagcgcggc gccgagcggg cccgccacgt
     2521 ccacgtcggc ccaccccggc ttgggggtgg cgaggattcg cccgtaggtg ggcgagcagg
     2581 gatcggggtc gagcgggccg aagcaggcga cgccaagcga ggcgacgggg ttctgccgga
     2641 agaactcgat cgcccgtgag atcgtctcgg ccggcgtggt ggtggggaag cgcgtttccg
     2701 cgcggatgtc gtccggcccc gtcccggccg cacagacgaa cttggtgccg ccgccctcga
     2761 tgccgccgta cagcgcttca cgctccaagc gagtctcccc gatccgtcgc acccggccgc
     2821 cgggcgccga cacgccattc tacagctggc ggcccagtag cacaggcgtc ccgcctgtgc
     2881 agccagcggg gggcgtcccg cctgtgcagc cagcggcagg cgtcccgcct gtgcagccag
     2941 cggcgggcgt cccgcctgtg cagccagcaa cgggcgtccc gcctgtgcgg ccagcggggt
     3001 gtccccttgt gtgtccagtg ggcagcgcgc ccagctcacc ggctggccgc ggggtagaac
     3061 cccagcacct tcaacgacag gcactgcgaa cggagcgagg cgagggcgct ctccacctcg
     3121 gggtcgccgg gtcggccgat gaagtcgatc aggaacgcgt aggtgccggg acggatcctc
     3181 gtcggcctcg actcgatgcg cgagaggttg aggccgcggg cggcgaagac gccgagcgcg
     3241 tcagcgagcg ccccgggccg gtcctcggcc gtgagcacga gcgagccctt g
//
LOCUS       DAOPQX010000402         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_31634_length_3288_cov_4.750387, whole genome shotgun sequence.
ACCESSION   DAOPQX010000402 DAOPQX010000000
VERSION     DAOPQX010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31634_length_3288_cov_4.750387"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            554..2587
                     /locus_tag="PKK95_15790"
     CDS             554..2587
                     /locus_tag="PKK95_15790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOC19731.1"
                     /translation="MTEPRAGAALTEPFDTSRLPDNAYTVLGPGEAYRPVVPPPARVP
                     EGTVRSVLWGLFLCIVFTLASAYSGLKVGQVMEASIPISILAIGLARVYRRRSTLLEN
                     VILTNIGGTSGAVVAGAVFTLPALYILGLDPHPLQTILICLAGGCLGVLFLIPLRRYF
                     VREMHGQFPFPEATAITEVLVTGEKGGSQAKLLLKATALAGAYDLLVTTFQVWKEYVD
                     LQFLPWVRTLADRARMATSFDAQVFILGLGYVIGLRSSAILVAGGLLSNFVLVPLIWM
                     VGSHLGEAAVYPGTTPIAGMSAGEIFRNYVRFIGVGAIATAGIFGIVKSLRVVAGSLS
                     IAFRAFRAGEGAHAFERTDRDIPLMSILIGVVVSTLAVAAFFTSLSGSPAVIAAGLVL
                     TLVFSFFFASVAANAIATIARNPVSGMTMLTIIISSVVLLRFGLSGPTGMFFVMAIAG
                     MVCTALSVSGQTITDLKTGYWLGSTPAAQEKVKFLGVLASALAVGLAIVILAVSFQFG
                     EQAAGDTRPILAAPQASIMKALVQGFMSQQPVAWMLFGVGAMIAVMMELLKVSPLTFA
                     LGMYLPLELNLPALVGGFIAHNVDRRADRTGGERGRTIRERGVILASGFMAGGALGGM
                     LGASLRLIPGFSEGWIRTPFFEYDPVSQTVSLGLFVAACVYLWWDATRKGAGI"
     gene            complement(2598..3263)
                     /locus_tag="PKK95_15795"
     CDS             complement(2598..3263)
                     /locus_tag="PKK95_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOC19732.1"
                     /translation="MAARPIRVLIADDHRMFREAVRAMLEPEPDIAVAGEAGTVAETL
                     QMVGRVPADVLLLDLALPDGHGLDVLSALAREGSPIHPIVVSASTDRQDVVRAIKAGA
                     RGVVLKDSHPDRLVEAIRQVASGQLWFGHQRLADLLDSLRQPGPAPGERPLDTLTRRE
                     RDIVAAIVQGASNKDISQQFGLRESTVKNHLTHIFDKLGVANRLELALFAVQHNLFEG
                     SGR"
BASE COUNT          440 a         1098 c         1155 g          595 t
ORIGIN      
        1 tgtgaaggtc acggtcgctg ggcccgttcc cgacgagggg ctggtcttca ggaagccggc
       61 gtcgaagctc gtcttccact tgcagctggc gtccgtgtcc agcgtgaagg tgccgctccc
      121 ggcggcagcc ggcaccgtca ccgcggaggg cgagatggtg tagcggcagg gagcaggggc
      181 cgcctgcgcg gcgcacaccg cgaatgcgct gagcattccg gtcaacaaca gcgcggcgtg
      241 agacggacga atcgtgacga tgctgcgcgt acgcacccct ggcctccctg gtggcggtgt
      301 cttgtcgtgg tggaagtggc cggcgctgga atcgctacgg acgagccggc tgcgggcgcg
      361 tgcgggcaag gggaatgccg agcttgaagc ccgtcaaaat cccgtttaaa ttgctatggt
      421 tgggcttaca gctcaacagc aaatacgtaa tcacacttac gttgttgtca tcctggcatc
      481 tccgtctagc gctggctcct cgcccccaaa gccactggcc cccaagcccg cagcccgagt
      541 agaatccgcc gctatgacag aaccacgcgc aggcgcggcg ctgaccgagc cgttcgacac
      601 ctcgcggctg cccgacaacg cctacaccgt gctcggtccg ggcgaggcgt accgcccggt
      661 cgtgccgcct cccgcgcggg tccccgaggg gaccgtgcgc tcggtgttgt gggggctctt
      721 cctctgcatt gtcttcacgc tggcgtcggc ctactcgggg ctgaaggtgg gccaggtgat
      781 ggaggcctcg atcccgatct cgatcctggc catcggcctc gcccgggtct accggcggcg
      841 atcgacgctg ctcgagaacg tcatcctcac caacatcggc ggcacctccg gcgcggtggt
      901 ggcgggtgcg gtcttcacgc tgccggcgct ctacatcctc ggcctcgatc cgcaccccct
      961 gcagacgatc ctgatctgcc tggcgggcgg atgcctcggc gtcctcttcc tgatcccgtt
     1021 gcggcgctac ttcgtgcgcg agatgcacgg gcagtttccc ttccccgagg cgaccgcgat
     1081 caccgaggtg ctcgtcaccg gcgagaaggg cgggtcgcaa gcgaagctgc tgctgaaggc
     1141 cactgcgctg gccggcgcgt acgacctgct ggtcaccacg ttccaggtgt ggaaggagta
     1201 cgtggacctg cagttcctgc cgtgggtgcg gacgctcgcc gaccgggcgc gcatggccac
     1261 cagcttcgac gcgcaggtct tcatccttgg gctcggctac gtcatcggcc tccgttcctc
     1321 ggccatcctc gtggccggcg gcctgctatc gaacttcgtc ctggtgcccc tcatctggat
     1381 ggtcgggagc cacctgggcg aagcggcggt ctaccccggg accacgccca tcgcgggcat
     1441 gagcgccggc gagatcttcc ggaactacgt ccgcttcatc ggtgtcgggg cgatcgctac
     1501 cgcggggatc ttcggcatcg tcaagtcgct gcgggtggtc gccgggtcgc tctcgatcgc
     1561 gttccgcgcg tttcgcgccg gggagggcgc gcacgccttc gagcgcaccg accgcgacat
     1621 ccccctgatg tcgatcctca tcggcgtggt ggtgtcgacg ctcgccgtcg cggcgttctt
     1681 cacgagcctg tcgggctctc cggccgtcat cgccgccggt ctcgtcctca cgctcgtctt
     1741 ctcgttcttc ttcgcctcgg tggccgccaa cgcgatcgcc accatcgcgc gcaacccggt
     1801 ttccgggatg acgatgctga cgatcatcat ctcgtcggtg gtcctgctgc ggttcgggct
     1861 gagcggcccc accgggatgt tcttcgtgat ggcgatcgcc gggatggtgt gcaccgcgct
     1921 gtcggtctcg ggccagacca tcaccgacct gaagaccggc tactggctgg ggtccacgcc
     1981 ggcggcgcag gagaaggtga agttcctcgg ggtgctcgcg tcggcgctgg ccgtggggct
     2041 ggcgatcgta atcctggcgg tcagcttcca gttcggcgag caggccgccg gcgacacgcg
     2101 gccgatcctg gccgcgccgc aggcctcgat catgaaggcg ctcgtgcagg gcttcatgag
     2161 ccagcagccg gtggcctgga tgctgttcgg ggtgggggcc atgatcgcgg tgatgatgga
     2221 gctgctcaag gtttctccgc tcaccttcgc gctcgggatg tacctgccgc tcgagctgaa
     2281 cctgccggcg ctggtcggcg gcttcatcgc gcacaacgtc gaccgtcgcg ccgaccgtac
     2341 cggcggcgag cggggccgca cgatcaggga gcggggggtg atcctcgcat cgggcttcat
     2401 ggccggcggc gccctgggcg ggatgctggg cgcctcgctc cgcctcatcc ccggcttcag
     2461 cgagggctgg atccgtacgc cattcttcga gtacgacccg gtctcgcaga cggtctcgct
     2521 cggcttgttc gtggcggcgt gcgtgtactt gtggtgggat gcgacgagga agggggctgg
     2581 gatctagacg gcgggtctca ccgtcccgat ccctcgaaca ggttgtgctg gaccgcgaag
     2641 agcgccagct cgagcctgtt ggcgacgccc agcttgtcga agatgtgcgt caggtggttc
     2701 ttgacggtgc tctcgcgcag gccgaactgc tggctgatgt ccttgttgct cgccccttgc
     2761 acgatggcgg cgacgatgtc gcgctcgcgg cgagtcaggg tatcgagggg acgctccccg
     2821 ggcgcggggc cgggttgccg gaggctgtcg agcaggtcgg cgagccgctg gtggccgaac
     2881 cagagctgcc ccgacgcgac ctggcggatg gcctcgacca ggcgatcggg gtgagagtct
     2941 ttgaggacga cgccgcgggc gccggccttg atggcccgca ccacgtcctg gcggtcggtg
     3001 gacgccgaca ccacgatcgg gtggatcgga gacccttcgc gggccagggc cgacaggacg
     3061 tcgaggccgt ggccgtcggg aagggcgagg tcgagcagca gcacgtcggc gggcacgcgc
     3121 cccaccatct gcagcgtttc ggcgaccgtg cccgcctcgc cggcaacggc gatgtcgggc
     3181 tcgggctcca gcattgcgcg gacggcttcg cggaacatcc ggtggtcgtc ggcaatcagg
     3241 acgcggatcg gtcgtgcagc catacgtctt cactcgccgt cgttccac
//
LOCUS       DAOPQX010000403         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_32208_length_3244_cov_3.928191, whole genome shotgun sequence.
ACCESSION   DAOPQX010000403 DAOPQX010000000
VERSION     DAOPQX010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32208_length_3244_cov_3.928191"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..978)
                     /locus_tag="PKK95_15800"
     CDS             complement(34..978)
                     /locus_tag="PKK95_15800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HOC19733.1"
                     /translation="MSMQVNGLHHVTAIAGDAQQNLDFYADVLGMRLVKRSVNQDDPG
                     TYHLFYADAAGSPGSDLTFFPWSQLAPPRPGHGLALEVALEVPPGSLDYWEARLRRLG
                     AGVFERERRFGELALPLQDPHGLRLVLVEASAPRRFEAWGGSPVPAERQVRGLYGARL
                     AERETAPTVAFLERALGMQRLAEENGWERYGVPGTAGVLDLRAMPDARRGAWGVGAVH
                     HLAWRVDDEAHQAEMRSRAEAAGAVPTPVIDRFWFKSVYFKEPGGVLFELATDGPGFA
                     VDEDAERLGETLVLPPWLERQRAAIEAALPPLVGVGSN"
     gene            1053..2675
                     /locus_tag="PKK95_15805"
     CDS             1053..2675
                     /locus_tag="PKK95_15805"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOC19734.1"
                     /translation="MPESRLEIKDALGRRVVPIDKEVFTIGRRVGNDLRLAGSDISRE
                     HAELVRRGDELRIRDRGSRFGTFVNGERVSEQALHPGDLIRLGQSGGVEMKYDEDDGG
                     RTRAGSSTEHPSSMGADFRQVATLLEGLRALGSARVLDEVLAMVIDSAIEMSGAERGF
                     IMLADPAGRLEFKLARARGRVTLPGRTFETSRKIPEAVFREGEFRMVADLLDGNLPAA
                     HTGTVALGIRHVFCIPLKLVRLADTPESSFEERRIGVLYLDSRERGTLVSGAVRSGME
                     TLATEAAVAIENARLYREAVEKARMEQEMRIAAEIQQALLPPPSWSTDYFEAEGRTLP
                     CRAIGGDFFDYLRCADGAAAFALADVSGKGAPAALLTAMIQGVLSAQAAFSGGPAAVL
                     TRVNEVLLQRALGSRFVTLFLAALTPAGVLRYCNAGHNPPFLVSGGSARRLETGGLIC
                     GLFPSATYQEETLQLLPGDTLVAFSDGVSEAFNAEGEEFGDRRILECVMGSLGAGVTG
                     LVASLLDALHAFTAGTVQGDDITVVAVRYNGQ"
     gene            complement(2705..>3244)
                     /locus_tag="PKK95_15810"
     CDS             complement(2705..>3244)
                     /locus_tag="PKK95_15810"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOC19735.1"
                     /translation="MDDPVAPTPRWLRICFPIVVTVVAALLVPAATALIGLLMFGNLL
                     RECGVTERLTRSSQNELINLVTILLGLCVGVTMDGNTFLRLSTIKILVLGCVAFTFST
                     AGGVLLGKLMARLPGSPVNPLIGAAGVSAVPMAARVAHNVGQETDPDNYLLMHAMGPN
                     VAGVIGTAIAAGALLALLG"
BASE COUNT          470 a         1159 c         1154 g          461 t
ORIGIN      
        1 acttccgccc ctaccgggct cccggcacga cttctaatta ctcccgaccc ctactagcgg
       61 cggtagcgcg gcctcgatcg cggcccgctg gcgctcgagc catgggggca gcaccagcgt
      121 ctcgcccagg cgctcggcat cctcgtcgac ggcgaacccg gggccgtcgg tcgccagctc
      181 gaacagcacc cctccaggct ccttgaagta caccgacttg aaccagaacc ggtcgatgac
      241 cggcgttggc accgcccccg ccgcctctgc ccggcttcgc atctcggcct ggtgcgcctc
      301 gtcgtccact cgccaggcga ggtggtgcac ggcgccgacg ccccaggcac cccgccgggc
      361 atcgggcatc gcgcgcaggt cgagaacacc cgcggtgccg ggcacgccgt accgttccca
      421 tccgttctct tctgcaaggc gctgcattcc cagcgcgcgc tcgaggaacg ccacggttgg
      481 ggccgtctcg cgctcggcga gccgcgcccc gtacaagccg cgaacctgcc gctcggcagg
      541 gaccgggctc ccgccccacg cctcgaaccg ccttggcgcc gacgcttcca cgaggacgag
      601 acgcaggccg tgcgggtcct gcagcggcag cgcaagctcg ccgaaccggc gctcgcgctc
      661 gaagacgcca gctcccaggc ggcgcagcct cgcctcccag tagtcgaggc tgccgggcgg
      721 tacctccagg gcgacctcca gcgccagccc gtggccgggg cggggtggcg cgagctggga
      781 ccacgggaag aaagtcaggt ccgagcccgg gcttcccgcc gcgtccgcgt agaagaggtg
      841 gtacgtgccg gggtcgtcct ggttgacgct gcgcttgacc agccgcatgc ccagcacgtc
      901 ggcgtagaag tcgagattct gctgggcgtc gccggcgatg gccgtgacgt ggtggaggcc
      961 gttgacctgc atgctcataa gattcagatt tccgcctgca ggtaacaggc cgggcctcgt
     1021 gtagagtgga aaggagaccc agggaggcca cgatgcccga gtcacggctc gagatcaagg
     1081 acgccctcgg ccggcgggtg gtcccgatcg acaaggaggt gttcaccatc ggccggcgcg
     1141 tcggcaacga cctgcggctg gccgggtcgg acatctcccg cgaacacgcc gagctggttc
     1201 gccggggcga cgagttgcgg atccgcgacc gggggtcgcg tttcggcacc ttcgtcaacg
     1261 gcgagcgggt gagcgagcag gcgcttcacc caggcgacct catccggctt gggcagtcgg
     1321 gcggcgtcga gatgaagtac gacgaggacg acggcgggcg cacccgcgcc ggaagctcca
     1381 ccgagcaccc ttcgtcgatg ggggctgact tccggcaggt ggccacgctg ctcgaggggc
     1441 tccgcgcgct cgggtcggcc cgcgtgctcg acgaggtgct ggcgatggtc atcgactcgg
     1501 ccatcgagat gagcggggcc gagcggggct tcatcatgct cgccgacccg gccgggcgcc
     1561 tcgagttcaa gctcgcgcgc gcgcggggcc gggtgacgct gcccgggcgc accttcgaga
     1621 cgagccggaa gattcccgag gcggtgttcc gagaggggga gttccggatg gtggccgacc
     1681 tgctcgacgg gaacctgcct gcggcccaca ccggcaccgt cgccctgggc atccgccacg
     1741 tcttctgcat tcccttgaag ctggtgcggc tcgccgacac gccggaatcc tcgttcgagg
     1801 agcgccggat cggcgtgctc tacctggaca gccgcgagcg gggcacgctg gtgtcgggcg
     1861 ccgtgcgcag cgggatggaa acgctggcca ccgaggcggc ggtggccatc gagaacgcgc
     1921 gcctgtaccg tgaggcggtt gagaaggccc gcatggagca ggagatgcgg atcgccgccg
     1981 agatccagca ggctttgctg ccgccgccct cgtggtcgac cgactacttc gaggccgagg
     2041 ggcggacgct gccgtgccgc gccatcggcg gcgacttctt cgactacctg cgctgcgccg
     2101 acggcgccgc cgcgttcgcc ctggccgacg tctccggcaa gggcgcaccg gccgccctcc
     2161 tcacggcgat gatccaggga gtgctgagcg cccaggccgc cttctccggc gggcccgccg
     2221 cggtgctgac gcgcgtcaat gaggtgctgc tgcagcgcgc gctcggctcg cgcttcgtca
     2281 ccctgttcct cgccgccctc acgccggccg gcgtcctccg ctactgcaac gccggccaca
     2341 accccccgtt cctggtgagc ggcggcagtg cgcgccgcct cgagacgggg ggactgatct
     2401 gcggcctgtt cccgtcggcc acctaccagg aggagacgct gcagctgctg cccggcgata
     2461 cgctggtggc gttcagcgac ggggtgagcg aggcgttcaa cgcggaaggg gaggagttcg
     2521 gcgaccggcg catcctcgag tgcgtcatgg ggtcgctcgg cgccggcgtc accggcctgg
     2581 tggcctcgct gctcgacgcg ctgcacgcgt tcaccgcggg caccgtccag ggcgacgaca
     2641 tcaccgtggt ggcggtgcgc tacaacgggc agtaaagcgt aggggcggcc cttgtggccg
     2701 ccctctaacc caacaacgcc agcagcgccc ctgccgcgat cgcggtgccg atgacgcctg
     2761 ccacgttggg ccccatcgcg tgcatcagca ggtagttgtc cgggtcggtc tcctgcccga
     2821 cgttgtgcgc cacgcgcgcg gccatcggca cggccgacac gccggccgcc ccgatcagcg
     2881 ggttgaccgg gctccccggc agcctggcca tcagcttgcc cagcagcacg ccgcccgcgg
     2941 tcgagaacgt aaaggccacg cagccgagca cgaggatctt gatggtggac agccgcagga
     3001 acgtgttccc gtccatcgtc acgccgacgc agagcccgag gaggatggtg acgaggttga
     3061 tcagctcgtt ttgcgacgag cgcgtcaacc gctcggtcac gccgcactcg cgcagcaggt
     3121 tgccgaacat cagcaggccg atgagcgcgg tggccgcggg cacgagcagg gccgccacca
     3181 cggtgacgac gatggggaag cagatccgca gccagcgggg cgtcggcgcg acggggtcgt
     3241 ccat
//
LOCUS       DAOPQX010000404         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_32660_length_3209_cov_4.423272, whole genome shotgun sequence.
ACCESSION   DAOPQX010000404 DAOPQX010000000
VERSION     DAOPQX010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32660_length_3209_cov_4.423272"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /gene="hscA"
                     /locus_tag="PKK95_15815"
     CDS             complement(<1..1422)
                     /gene="hscA"
                     /locus_tag="PKK95_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOC19736.1"
                     /translation="MSHVVGIDLGTTNSLVAWVRDGEPTVIPDPVSGEVLLPSIVSVD
                     DRGNVHVGREAQRRLLTDAGHTVYSVKRFMGRGASDVADEASHFPFSLAGEGEGVVRI
                     GLGERAFTPPEISAFILRELKRRAEAYFQERGEFDFEVDRAVITVPAYFNDAQRTATR
                     DAGRIAGIEVLRIINEPTAASLAYGLDRRRGGTIAVYDFGGGTFDISILRVEDGVFQV
                     LATNGDTHLGGDDIDRLLVEQVVSEMGAELTPEVLQAVRLAAIQAKIDLSAQEQTELR
                     VYLAGRGAAPVYRRALDRASFERLIAPVVERTLVPCRAALADASLGPGQVDEVVMVGG
                     STRIPFVRRAVEELFGRRPHTELNPDEVVAIGAAVQANILVTGNRDMLLLDVTPLSLG
                     IETMGGAMEKIILRNSTIPATGTEMFTTFVDNQTAVDIHVLQGERELVRDNRSLARFR
                     LRGIPPMPAGLARLQVRFQIDANG"
     gene            complement(1430..2173)
                     /gene="hscB"
                     /locus_tag="PKK95_15820"
     CDS             complement(1430..2173)
                     /gene="hscB"
                     /locus_tag="PKK95_15820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOC19737.1"
                     /translation="MPADSRALVLQSCRHCGAGAPAEVHFCPQCDRILTLARHGDYFT
                     FFGLPRKLTIDLADLERRFRDLSRQFHPDYFYNATAAERLASLERSSYLNDAYRVLRQ
                     PIDRVEYLLKLEGLAPAGRQQSGADASRLEPGLLEEVFELNEELDAIRRARAAGASPD
                     EVARRLEAAARPIETRAARQETRLAALMARWDELAEEAGPNACATGEGAGSAAKRQTL
                     EALRALLHERSYVDNLLQLLARERGEPRA"
     gene            complement(2238..2567)
                     /locus_tag="PKK95_15825"
     CDS             complement(2238..2567)
                     /locus_tag="PKK95_15825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOC19738.1"
                     /translation="MGIQVSDVAARRIKLLMEKQQMTEGGLRVGVKGGGCSGLSYTFS
                     WERQARLGDEVIDGPDGVKIFVDRKSLIYLNGTVLDYDTSLLSKGFIFQNPNAKSTCG
                     CGSSFQV"
     gene            complement(2567..3022)
                     /gene="iscU"
                     /locus_tag="PKK95_15830"
     CDS             complement(2567..3022)
                     /gene="iscU"
                     /locus_tag="PKK95_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOC19739.1"
                     /translation="MSYSDKVLEHYNNPRNVGSLPKEDDTVGTGLVGAPECGDVMKLQ
                     VKVDPETGVIADAKFKTFGCGSAIASSSLATEWLKGKTLDQALEIKNVDIVNELSLPP
                     VKIHCSVLAEDAIKAAIADYKRKQAEAGQGNGNGAAPAGGSGTAAAGAR"
BASE COUNT          469 a         1156 c         1081 g          503 t
ORIGIN      
        1 cccgttggcg tcgatctgga accgcacctg cagccgcgcc agcccggccg gcatcggcgg
       61 gatgccgcgc aggcggaagc gcgcgagcga ccggttgtcg cgcaccagct cgcgctcgcc
      121 ctggagcacg tggatgtcca ccgccgtctg gttgtccacg aaggtcgtga acatctcggt
      181 cccggtcgcc ggaatcgtcg aattgcggag gatgatcttc tccatcgccc cgcccatcgt
      241 ctcgatgccg agcgagagcg gggtgacgtc gagcagcagc atgtcgcggt tgccggtcac
      301 caggatgttg gcctgaaccg cggcgccgat cgccaccacc tcgtcggggt tcagctcggt
      361 gtgcggccgc cgcccgaaca gctcctccac ggcgcggcgc acgaacggga tgcgcgtgga
      421 gccccccacc atcaccacct cgtccacctg ccccggcccg agcgaggcgt cggccagcgc
      481 ggcccggcag ggcacgagcg tgcgctcgac gaccggcgcg atcagccgct cgaaggaggc
      541 gcggtcgagg gcccggcggt agaccggcgc agccccccgc cccgcgaggt acacgcggag
      601 ttccgtctgc tcctgcgccg agaggtcgat cttcgcctgg attgccgcca gccggacggc
      661 ctgcagcacc tcgggcgtca gctcggcgcc catctccgat acgacctgct cgaccaggag
      721 acggtcgatg tcgtcacccc cgaggtgcgt gtccccgttg gtggccagca cctggaacac
      781 cccgtcctcc acccggagga tcgagatgtc gaaggtgccg ccgccgaagt cgtagacggc
      841 gatcgtgccg ccccgccgcc gatcgaggcc gtacgccagc gaggcagccg tcggctcgtt
      901 gatgatccgc agcacctcga tgcccgcgat ccggccggcg tcgcgcgtcg cggtgcgctg
      961 tgcgtcgttg aagtaagcgg ggacggtgat gacggcccgg tccacctcga agtcgaactc
     1021 gccgcgctcc tggaagtacg cctcggcccg ccgcttcagc tcgcgcagga tgaaggccga
     1081 gatctcggga ggcgtgaacg cccgctcgcc gaggccgatc cgcacgaccc cctcgccctc
     1141 gccggccagg ctgaagggga agtgcgacgc ctcgtcggcc acgtcggagg cgccgcgccc
     1201 catgaagcgc ttgacggagt agacggtgtg gccggcgtcg gtcagcaggc ggcgctgcgc
     1261 ctcccgcccg acgtggacgt tgccccgatc gtccaccgac acgatcgagg ggaggagcac
     1321 ttctcccgag acggggtcgg gaatcaccgt cggctcgccg tcccgcaccc aggcgacgag
     1381 gctgttggtg gtgcccagat cgatgccgac gacgtgggac atatgagggt tacgcgcgcg
     1441 gctcgccccg ctcgcgggca agcagctgca ggaggttgtc gacgtaactg cgctcgtgaa
     1501 gcagggcgcg aagggcctcg agcgtctggc gcttcgccgc cgagcctgcg ccctccccgg
     1561 tggcgcaggc gttcggtccg gcttcctccg ccagttcgtc ccaccgagcc atcaaggctg
     1621 cgaggcgcgt ctcctgccgc gccgcgcgcg tttcgatcgg ccgggcggcg gcctccaggc
     1681 gccgggccac ctcgtcgggg gatgctccgg ccgcccgggc acggcggatg gcgtccagtt
     1741 cctcgttcag ctcgaacact tcctcgagca gcccgggctc gaggcgcgag gcgtcggcgc
     1801 ctgactgctg gcggccggcc ggggccagcc cttccagctt gagcaggtac tcgacccgat
     1861 cgattggctg ccgcagcacg cggtaggcgt cgttcaggta cgacgagcgc tccaggctgg
     1921 ccaggcgttc ggcagccgta gcgttgtaga agtagtcggg gtggaactgc cggctgaggt
     1981 ctcggaagcg gcgctccagg tcggcgagat cgatcgtcag cttgcgcggc aggccgaaga
     2041 acgtgaagta gtcgccgtgc cgcgccagcg tcaggatgcg gtcgcactgc gggcagaaat
     2101 ggacttccgc cggcgccccc gccccgcagt ggcggcagga ctggaggacc agcgcacggg
     2161 aatcagcagg catgtgatga gaacgaaaac cggtgaaacg cgaaccggag aacctcgaac
     2221 gttccgcgcg gcccgcctca cacctggaac gacgagccgc agccgcacgt gctcttggcg
     2281 ttcgggttct ggaagatgaa ccccttgctg aggagcgacg tgtcgtagtc gagcaccgtt
     2341 ccgttcaggt agatgaggct cttgcggtcg acgaagatct tgacgccgtc cggaccgtcg
     2401 atcacctcgt cgccgagccg ggcctgccgc tcccacgaga acgtgtagct caggcccgag
     2461 cagccgccgc ccttgacgcc cacccgcagg ccgccctcgg tcatctgctg cttctccatc
     2521 agcagcttga tccgccgggc cgccacgtcg ctgacctgga tgcccattac cgtgctcccg
     2581 ccgcggccgt gccgctgcca ccggccggcg cggccccgtt gccgttgccc tgccccgcct
     2641 cggcctgctt ccgcttgtag tcggcgatgg ccgccttgat cgcgtcctcg gccagcaccg
     2701 agcagtggat cttcaccggc ggcaggctca gctcgttgac gatgtcgacg ttcttgatct
     2761 cgagcgcctg gtccagcgtc ttgcccttca gccactcggt ggccaggctc gagctggcaa
     2821 tcgccgaccc gcagccgaac gtcttgaact tcgcgtccgc gatgacgccg gtctcgggat
     2881 ccaccttgac ctgcagcttc atcacgtccc cgcactcggg cgcgcccacc aggccggtcc
     2941 cgaccgtgtc gtcctccttc ggcagcgagc cgacgttgcg ggggttgttg tagtgttcga
     3001 gaaccttgtc cgagtaagac atcgtcatcc ccgcagcccg cgagcaatct tagatggctg
     3061 ccctgccaga ggctttctcg cgcgccagct cgtacagcgg cgacatctcg cgaagcctcg
     3121 tcactacctg caccatcttc ccggcgacgt agtccacctc ctcctcggtg ttgaaccggc
     3181 cgaggccgaa gcggatggac gagtgcgcc
//
LOCUS       DAOPQX010000405         3199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_32800_length_3199_cov_3.757952, whole genome shotgun sequence.
ACCESSION   DAOPQX010000405 DAOPQX010000000
VERSION     DAOPQX010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32800_length_3199_cov_3.757952"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKK95_15835"
     CDS             <1..406
                     /locus_tag="PKK95_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19740.1"
                     /translation="GTLTTWLLAGSFFGFLGGALPEAHGEHAVGSTSALLVQIATAPA
                     TGLALLVVALGLLCYWQRARLSGPAAALDGIGALARHNFGFEAINRGIVGATQGAAES
                     LRATQPGILNWNVMAILVGLVVVLAILALGGA"
     gene            412..1941
                     /locus_tag="PKK95_15840"
     CDS             412..1941
                     /locus_tag="PKK95_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOC19741.1"
                     /translation="MNASTALIWLLAMPLVASAGVYLLGRIGVRVSSSAAPARWLAAI
                     TLAACGLPLWIAGQAVLGAGSVSVGFGEVGLRMDGVGLLLAATVLVLTFAVSLFSVPY
                     MRGEEGEEKYYALLVAMATSMIGLGCADDLFNLWVWFEAMAVTSFLLVAFYREQKASL
                     EAGVKYLVQSAVGSAFVLIGIALVFGRTGTLRLPEILAVVSSTGATPGLLAAGALFLI
                     GFGVKTALVPLHTWLPDAHSMAPSGISAMLSGVVIEAGLVAMLRSLGALAPVASTWGA
                     LILAFGALNMIVGNLMALRQTQVKRLLAYSSVSHMGYMLLGFGVAVGYGIANGAAGGF
                     FHLANHALMKGLAFMAVGALLYSMKIANGSHEPLMVHDLDGASNRYPVTAFTLSVALL
                     ALGGLPPLSGFMSKWQIMVAGFETHNAGVEALVIFAAINSVLSLGYYAPLVNRMYRNE
                     PAPAVAAGRPVTPLLGAPLVVLTLVIAALGFWPGMVDFVTGPAGEQLVSIFAGPPAWG
                     N"
     gene            1955..2266
                     /locus_tag="PKK95_15845"
     CDS             1955..2266
                     /locus_tag="PKK95_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19742.1"
                     /translation="MEILLSPPVAFLAYIPLVLVLVGLGRLLAGPSNATPMKTSIYGS
                     GEAPPTFEAAPGYQPFFLIALFFAMVHLAMLVLGLGSLPTPAAVYLLGLFVALIALIL
                     G"
     gene            2276..2743
                     /locus_tag="PKK95_15850"
     CDS             2276..2743
                     /locus_tag="PKK95_15850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOC19743.1"
                     /translation="MAGTDSSMLDAILNPLRRWARINSPWAIHYNSGSCNGCDIEILA
                     TLTPRYDIERFGIKLQGSPRHADVLICTGPVTRQSRDRLVRIYEQMPDPKFVVAVGSC
                     GLTGGAFHGCYNIVGSLGEVIPVHAMVPGCPPRPEAIIYGVVALLNSLKPKAA"
     gene            2784..>3199
                     /locus_tag="PKK95_15855"
     CDS             2784..>3199
                     /locus_tag="PKK95_15855"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOC19744.1"
                     /translation="MKLEELLTTAETLLAPWTVGLTRFESRRLDVVVKRDDLVAAAGA
                     LVDARWGYLSAITGLDLPWPKTPPKTDAPPAPPLEDQLEALYHFVEGAAVVTLRVRVP
                     YSDARVPSICGPVPTATLYERELREMFGIEVVGTPVT"
BASE COUNT          398 a         1122 c         1126 g          553 t
ORIGIN      
        1 ggggacgctg accacgtggc tgctggccgg cagcttcttc gggttcctcg gcggcgcgct
       61 ccccgaggcg cacggcgagc acgcggtcgg ctcgacctcg gcgctgctcg tgcagatcgc
      121 gacggcgccg gcgaccgggc tggcgctgct ggtcgtggcc ctcggccttc tctgctactg
      181 gcagcgggcg cggctgtccg ggccagccgc cgcccttgac ggcatcgggg cgctggcgcg
      241 ccacaacttc gggttcgagg cgatcaaccg cggcatcgtc ggggccacgc agggcgccgc
      301 cgagtcgctt cgcgccacgc agccggggat cctcaactgg aacgtgatgg ccatcctcgt
      361 cgggctggtc gtcgtgctgg ccatcctggc gctcggagga gcctaaggac catgaacgcg
      421 agcaccgcct tgatctggct gctggcaatg cccctggtgg cgtcggccgg cgtgtacctg
      481 ctggggcgca tcggggtgcg cgtgtcgagc tccgcggcgc cggcgcggtg gctggcggcc
      541 atcacgctgg ccgcgtgcgg cctccccctc tggatcgccg gccaggcggt cctcggagcc
      601 ggcagcgtgt cggtggggtt cggagaggtc ggcctcagga tggacggcgt gggcctgctg
      661 ctggcggcca cggtcctcgt gctcaccttc gccgtgtcgc tcttctcggt gccctacatg
      721 cgcggtgagg agggcgagga gaagtactac gccctgctcg tggcgatggc cacctcgatg
      781 atcggtctcg gctgtgccga cgacctgttc aacctctggg tctggttcga ggcgatggcg
      841 gtgacctcgt tcctgctcgt cgccttctac cgcgagcaga aggcatcgct cgaggcgggc
      901 gtcaagtacc tggtgcagtc ggcggtcggg tcggccttcg tgctgatcgg gatcgcgctg
      961 gtgttcggcc gcaccggcac gctgcggctt cccgagatcc tcgccgtcgt gtcgtccacc
     1021 ggggcgacgc cggggctgct cgccgccggc gccctgttcc tcatcggctt cggcgtgaag
     1081 accgccctgg tgccgctgca cacctggctg ccggacgccc actcgatggc cccgagcggc
     1141 atcagcgcaa tgctttccgg cgtggtgatc gaggcggggc tcgtcgcgat gctgcggtcg
     1201 ctcggcgcgc tggccccggt ggccagcacg tggggggcac tcatcctggc cttcggcgcg
     1261 ctgaacatga tcgtcggcaa cctgatggcc ttgcggcaga cgcaggtcaa gcggctgctc
     1321 gcctactcga gcgtgagcca catgggttac atgctgctcg ggttcggggt ggccgtcggc
     1381 tacgggatcg ccaacggcgc ggcgggcggc ttcttccacc tggccaacca cgccctcatg
     1441 aaggggctgg ccttcatggc ggtcggcgcg ctgctctact cgatgaagat cgcgaacggc
     1501 agccacgagc cgctgatggt gcacgacctc gacggcgcgt cgaaccgcta cccggtgacg
     1561 gcgttcacgc tgagcgttgc cctgctcgcc ctcggcggcc tgccgcccct gtcgggattc
     1621 atgtcgaagt ggcagatcat ggtcgccggg ttcgagacgc acaacgccgg cgtcgaggcg
     1681 ctggtcatct tcgcggccat caacagcgtc ctgtcgctcg ggtattacgc tccgctggtg
     1741 aaccggatgt atcgcaacga gcccgcaccc gcggtcgccg ccggcaggcc cgtgaccccg
     1801 ctgctcggcg cgccgctcgt ggtgctgacg ctggtgatcg cggcgctcgg cttctggccg
     1861 gggatggtcg acttcgtcac cggcccagcc ggcgaacaac tggtgtcgat cttcgccggt
     1921 ccgccggcgt ggggcaattg aggggggctt gactatggag atcctgcttt ctccgccggt
     1981 ggcgttcctg gcgtacatcc ccctggtgct cgtgctggtg ggcctcgggc ggctcctggc
     2041 ggggccctcg aacgcgacgc cgatgaagac gagcatctac ggcagcggcg aggcgccgcc
     2101 caccttcgag gccgcgcccg gctaccagcc gttcttcctg atcgccctgt tcttcgcgat
     2161 ggtccacctg gcgatgctgg tgctcggcct cggcagcctg ccgacgccgg ccgccgtgta
     2221 cctgctcggc ctcttcgtcg cgctgatcgc gctcatcctc ggataggagc tcttcatggc
     2281 cggtacggac agctcgatgc tcgacgccat cctgaacccg ctccgccgct gggcgcgcat
     2341 caattccccg tgggcgatcc attacaacag cgggtcgtgc aacgggtgcg acatcgagat
     2401 tctcgccacc ctgacaccgc gctacgacat cgagcgtttc gggatcaagc tccagggcag
     2461 cccgcggcac gccgacgtcc tcatctgcac gggccccgtc acccggcagt cgcgcgaccg
     2521 gctggtccgg atctacgagc agatgcccga cccgaagttc gtggtggcgg tcggctcgtg
     2581 cggcctgacc ggcggcgcct tccacggctg ctacaacatc gtcggcagcc tgggcgaggt
     2641 gatcccggtg catgccatgg tcccgggctg cccgccccgg cccgaggcga tcatctacgg
     2701 ggtcgtggca ctgttgaaca gcctgaagcc gaaggcggca tagggatctc ggagaggcgg
     2761 ggacgcctga tgggatggac gagatgaaac tcgaagagct gctgaccacc gccgagaccc
     2821 tcctcgcgcc ctggaccgtc ggcctcaccc ggttcgagag ccgccgcctc gacgtcgtcg
     2881 tcaagcgcga cgacctggtc gcggccgcgg gggctctcgt ggacgctcgc tggggctacc
     2941 tgtccgcgat caccggcctc gacttgccct ggcccaagac gccgcccaag accgacgcgc
     3001 cgcccgcccc gccgctcgag gaccagctcg aggcgcttta ccacttcgtg gagggtgccg
     3061 ccgtcgtgac cctgcgggtc cgcgtgccgt actccgacgc ccgcgtgccg agcatctgcg
     3121 gcccggtgcc gacggccacg ctgtacgagc gcgagctgcg cgagatgttc gggatcgagg
     3181 tcgtgggaac gcccgtgac
//
LOCUS       DAOPQX010000406         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_33089_length_3178_cov_4.969581, whole genome shotgun sequence.
ACCESSION   DAOPQX010000406 DAOPQX010000000
VERSION     DAOPQX010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33089_length_3178_cov_4.969581"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PKK95_15860"
     CDS             <1..814
                     /locus_tag="PKK95_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOC19745.1"
                     /translation="LFAARRLWRRRLARAGSDRRALLSGVVPEAGDRASCALTVLEED
                     VLGLMREGDVPGWEIPGRYFADTRTGDASALEAVLEHNRLDLVSLGALTGVVLELLSG
                     RREAGDRYELLALGRLYDAVGCDGDAERCFRGAAAAGGLFEAALDAAARAEALHWLAL
                     RHRRARRFADAAACWADLLALPGLDPRLRREALEAMAVHNEHRARDLAAARRFALRAL
                     DLAPGGRRQEDLAHRLGRLSRKIAAAGPAAADPAAGPQVARDMFTPPPNAAS"
     gene            complement(766..969)
                     /locus_tag="PKK95_15865"
     CDS             complement(766..969)
                     /locus_tag="PKK95_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19746.1"
                     /translation="MADIDIVPKQRSYTWLWILLALVILAVLWWALAGSDDITTSPPA
                     GAIGSAVNQLAALGGGVNMSLAT"
     gene            complement(970..1560)
                     /locus_tag="PKK95_15870"
     CDS             complement(970..1560)
                     /locus_tag="PKK95_15870"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOC19747.1"
                     /translation="MADYNKDLKRFSEMDNVEVAKDDPDIRGWDVTTSDGQDLGEVKD
                     LLVDTRTMKVHAVEVELEGSRFNWNDNRRVVVPVDSLRVNRDDDEVVISGMRKDEIAA
                     LPASEASWGLGTDASSRPIASSEPGSYIAPDATRGRAGFDADVHGRSAEDTRGIGAER
                     ITRAEEELRAGKERLDASRESGEAGGRSRSERKGGR"
     gene            1882..>3178
                     /locus_tag="PKK95_15875"
     CDS             1882..>3178
                     /locus_tag="PKK95_15875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOC19748.1"
                     /translation="MNERADRPSPARARARLPDLSDWKAQVKCQAGCPVATDAGRYVQ
                     LIAEGRDEDAFLVARAPNPFASVCGRVCAAPCEDACRRGSIDAPISIRALKRYVTEQY
                     GVESVRADTQDRLRTSLVAEGNRYAGHLPVAPVGAAGSRARSRRVAIVGAGPAGLSAA
                     HDLALLGYDVTVFDAARDPGGMMRFGIPEYRLPRTLLRAEIEKILALGVTLVPGTALT
                     PAFGLDALRREGFEAVFIAVGVSQGRDLQAPGAGLDGVVKAVDYLLNVNRGYRLDLGR
                     RVIVIGGGFVAFDAARTALRAAEEDADERAMLQDAADARVQEAFDSARAAIRGGAAEV
                     TVVSLETFDEMPVLRTTQGLEEFEEARKEGVKFVTRRGPKEFLGSGRLERVRLQRVLS
                     VFDANGRFAPAYDNGDVVELEADACILAIGQKADLSFLSP"
BASE COUNT          383 a         1166 c         1127 g          502 t
ORIGIN      
        1 gctcttcgcc gcccgccgcc tctggcgccg ccgcctggcg cgggcggggt ccgaccggcg
       61 cgccctgctg tcgggcgtcg tgcccgaggc cggcgaccgg gcgagctgcg cgctgacggt
      121 gctcgaggaa gacgtgctcg gcctgatgcg cgagggtgac gtgccgggct gggagattcc
      181 gggccggtac ttcgccgaca caaggaccgg agatgcctcc gccctcgagg ccgtgctcga
      241 gcacaaccgc ctcgacctcg tctcgctcgg ggcgttgacg ggggtcgtgc tcgagctgct
      301 ctcgggccgc cgggaggcgg gcgaccgcta cgagctgctc gcgctcgggc ggctgtatga
      361 cgccgtgggg tgtgacggtg atgcagaacg gtgcttccgg ggagccgcgg cggccggggg
      421 gctgttcgag gcggccctcg acgcggcggc gcgcgccgag gcgctgcact ggctggcgct
      481 ccggcacagg cgcgcgcgcc gctttgccga cgcggccgcc tgctgggccg acctgctcgc
      541 actccctggc ctcgacccac ggcttcgccg ggaggcgctc gaggcgatgg cggtccacaa
      601 cgagcaccgc gcgcgcgacc tggcggccgc gcgccgcttc gcgctgcgcg cgctcgacct
      661 cgcgcccggc gggcgcaggc aggaggacct ggctcaccgc ctcggccggc tctcccgcaa
      721 gattgccgcc gcggggcctg ccgcggcaga ccccgcggcc gggcctcagg tggccaggga
      781 catgttcacg ccgccgccca acgcggcaag ctgattgacg gcggagccga tcgctcccgc
      841 cggcgggctg gtcgtgatgt cgtcgctgcc cgccaacgcc caccagagca cggccaggat
      901 caccagcgcc agcaggatcc acagccacgt gtagcttcgt tgcttcggaa cgatatcgat
      961 gtctgccatc taacgtcctc ccttgcgttc cgagcgggaa cggccgcccg cctcgccgct
     1021 ctcgcggctc gcgtccagcc gctccttgcc ggcgcgcagc tcttcctccg cacgggtgat
     1081 ccgctcggcg ccgatcccgc gcgtgtcctc ggccgaccgc ccgtgcacgt cggcgtcgaa
     1141 tccagcccgg ccccgcgtgg cgtctggcgc gatgtacgag ccgggctcgg acgaggcgat
     1201 cggccgcgac gaggcatcgg ttccgagccc ccacgacgcc tccgaggcgg gcagggcggc
     1261 gatctcgtcc ttgcgcatcc cgctgatgac cacttcgtcg tcgtccctgt tgacccgcag
     1321 cgagtcgacc ggcaccacga cgcggcggtt gtcgttccag ttgaagcggc tgccctcgag
     1381 ctccacctcg acggcgtgta ccttcatcgt ccgcgtgtcg acgagcaggt ccttgacctc
     1441 gccgaggtcc tggccatccg acgtggtgac gtcccatccg cgaatgtcag ggtcgtcctt
     1501 ggcgacctcg acgttgtcca tctcgctgaa gcgcttgagg tccttgttgt agtccgccat
     1561 gcgcgttctt ccctctccct ttctctgtct ccgatccgtg gcttcttcgg gcgtcccgcg
     1621 caaacgtgca aggccggtgc ccgcatgccg gaatacttgc gctggctctg ccgcatgggg
     1681 taaggtgacg cgcacgagtc atcaacaact gtagttgcgc cgcgattggt cgctaaggat
     1741 ggtccggttc cggcgatggg cgccccgtcg ccggtcttgt gatccaggtc acagggggta
     1801 cgcgccgatc ggcatacact cccgtgagcc attcccagtc tgcgttttcc gccggcccgc
     1861 gtccgtttcc ggaggccgcg catgaatgaa cgcgccgatc gtccctcgcc cgcccgcgcg
     1921 cgcgccaggc tgcccgacct gtccgactgg aaggcgcagg tgaagtgcca ggcgggctgc
     1981 cccgtggcca ccgacgccgg ccggtacgtc cagttgattg ccgaggggcg cgacgaggac
     2041 gccttcctcg tcgcccgcgc ccccaacccc ttcgcctctg tgtgcggccg cgtgtgcgcg
     2101 gcgccgtgcg aggacgcctg ccgccgcggc agcatcgacg cccccatctc gattcgcgcg
     2161 ctcaagcggt acgtgaccga gcagtacggc gtcgagtcgg tgcgcgccga cacgcaagac
     2221 cgactgcgca cctcgctggt ggccgaggga aaccggtacg cgggccacct tccggttgcg
     2281 cccgtggggg ccgccgggag ccgcgcgcgg tcgcgccggg tcgccatcgt cggtgcaggg
     2341 cctgccggcc tctcggccgc gcacgacctc gcactcctcg ggtacgacgt gaccgtcttc
     2401 gacgccgccc gcgacccggg cggcatgatg cgcttcggga tccccgagta ccgcctgccg
     2461 cgcacgctgc ttcgcgcgga gatcgagaag attctcgcgc tcggcgttac gctcgtcccg
     2521 ggaacggcgc tcacgcccgc cttcggcctc gacgcgcttc ggcgcgaggg gttcgaggcc
     2581 gtcttcattg ccgtcggcgt ctcgcagggg cgcgacctgc aggcgccggg cgccggcctg
     2641 gacggcgtcg tcaaggcggt cgactacctc ctcaacgtga accgcgggta ccgcctcgac
     2701 ctggggcggc gggtgatcgt gatcggcggc ggcttcgtgg ccttcgatgc cgcccgcacg
     2761 gcgctgcggg ctgccgagga ggatgccgac gaacgggcga tgctccagga tgccgccgac
     2821 gcccgggtgc aggaggcgtt cgactcggcg cgtgccgcca ttcgcggcgg cgctgcggag
     2881 gtgaccgtcg tctcgctcga gaccttcgac gagatgccgg tgcttcgcac cacgcagggc
     2941 ctcgaggagt tcgaagaggc ccgcaaggaa ggagtgaagt tcgtcacgag gcgcggcccg
     3001 aaggagttcc tgggctccgg gcggctggaa cgggtccgcc tgcagcgcgt cctctcggtg
     3061 ttcgacgcga acggccgctt cgcgccggcc tacgacaacg gcgacgtggt ggagctcgag
     3121 gcggacgcgt gcatcctcgc catcgggcag aaggccgacc tctcgttcct gtccccgg
//
LOCUS       DAOPQX010000407         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_33500_length_3150_cov_4.178998, whole genome shotgun sequence.
ACCESSION   DAOPQX010000407 DAOPQX010000000
VERSION     DAOPQX010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33500_length_3150_cov_4.178998"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKK95_15880"
     CDS             <1..192
                     /locus_tag="PKK95_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19749.1"
                     /translation="LDGRFDVQVMQRLPFLDFTSARWQALVAVRNTFRDATADASVYD
                     ELLVVRPPKRFVGGLLVRF"
     gene            452..>3150
                     /locus_tag="PKK95_15885"
     CDS             452..>3150
                     /locus_tag="PKK95_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOC19750.1"
                     /translation="MPGVPWPFWAVAPNAITILVAAALLALATANLVSRAGWNPVEDG
                     VLWESRPEGVVASEVLPGTPAARAGVRPGDLLIAIDGAPIDRPADVEAAHDGRRAGDR
                     VKYTLARFGGTSLLEFDLRAVPQGNRGLYYVLAGVGIFTLLVATAVRVRRPGNPATLH
                     FFLLSTAFFGVFAFSHSGRFDRLDWVFYWADAASVLLLPPLFAHFTLFFPERPRPWLQ
                     GSLGAAVVPLLYLPALLLGGLRLWAVARASLDPPFYVGILQTLDRFEPLYLSLCLVGL
                     LVVARAFRQVRSVTARRQLRWIAWGTVLGALPFALGYALPFALGVRPTLSMELFAVPL
                     GLIPLAFASAIVRYRLMDVEVIVKRSMVWAAAIAAISATYAILLQLVTWAFLPNSRAP
                     NTILAVLATLVVVLLSRPLKNAIQTMLDKAFYRDRYDYRRALVGFARDLSSDLDLARL
                     SGRIVGRVTETLLVDRMAVLLWDERAGEFTSVRTAGLGGEIVRLPRQGTLGARLAAGH
                     TVDLDDARSTRGIEADEIDGWRERELYYFIPCVSKDAAIAVLALGRKAHAEPLSSEDM
                     ALLAAVAGQAATALENGRLYRQLHLKADELGRMRAFNESILESLEVGLTVVNENDQVV
                     RWNRALEALTGVSREQAIGRPLRELFHPAFYDPLRRARDQSPRGASLYRVSLPVGAEG
                     GGSRRLVNVTTVPLQPGEGNPANGTVILVEDVSARVQLEEQVQISEKMASIGLLAAGV
                     AHEVNTPLTGISSFTQMLLEGADPGDPKTRVLEKIERQTFRAAKIVNGLLNLSRPGAA
                     SAERAPVDLNVVINDVLSLLEHQFRVGHIQVRRELASPGPVVLGIEHKLQQVFLNLFL
                     NARDAMPKGGWLSIATRVADDQAVAEIADTGAGIPSDHLARIY"
BASE COUNT          396 a         1081 c         1144 g          529 t
ORIGIN      
        1 ctcgacggcc ggttcgacgt ccaggtgatg cagcgcctgc cgttcctcga cttcacgtcc
       61 gcccggtggc aggcgctggt ggccgtgagg aacactttcc gcgacgcgac cgccgacgcc
      121 tctgtctatg acgagctgct ggtggtccgt ccccccaagc gcttcgtcgg cggcctcctg
      181 gttcgcttct gagcggtgcc agcggcaggc gggccctccc ccggcccgct ctttttttgt
      241 ccgggtgtcg atccaaccac ttggatgagg tgtccgctct gttggatggc cgggggcaac
      301 gcgagccagc gccggctcga agacacgaag atggaaaggg ttacggtgca tccaagggct
      361 tggagcggtg gtatggtgct tgctgaactg ctcacttagc cttttccttg gccgagcgat
      421 gagcaaccag gccgtaagcg tgagtcgccg attgccgggc gtaccgtggc ccttctgggc
      481 ggtcgccccc aacgcgatca cgatcctggt tgcggccgcg ctcctcgcgc tcgcgacggc
      541 gaaccttgtt tcccgggccg gttggaaccc cgtcgaggac ggcgtgctct gggagtcgcg
      601 acccgagggc gtcgtcgcgt ccgaagtgct tccgggtacg ccggcggcac gggcgggagt
      661 gcggcctggc gatctgctga tcgcgatcga cggggcgccg atcgaccggc ccgccgacgt
      721 cgaggctgcg cacgacgggc gccgcgcggg cgaccgggtg aagtacacgc tggcgcggtt
      781 cggcggcacg tcgctgctcg aattcgacct gcgggcggtg ccgcagggga accgcgggct
      841 ctactacgtg cttgccgggg tcgggatctt cacgctgctc gtggcgacgg cggtgcgggt
      901 gcggcggccg ggcaaccccg cgacgctgca cttcttcctg ctgtcgacgg ccttcttcgg
      961 cgtattcgcc ttctcgcaca gcgggcggtt cgaccgcctc gactgggtct tctactgggc
     1021 ggacgccgcg tcggtgctgc tgctgccgcc gctcttcgcg cacttcacgc tgttcttccc
     1081 ggagcggccc cgcccctggc tgcaggggtc cctgggggcg gccgtcgtgc cgctgctgta
     1141 tcttccggcg ctgctgttgg ggggactgcg gctctgggcg gtcgcgaggg cgtcgctcga
     1201 cccgcccttc tacgtgggga tcctgcagac gctcgaccgg ttcgagccgc tgtacctctc
     1261 gctgtgcctc gtcggcctgc tcgtcgtggc gcgcgcgttc cggcaggtcc gatcggtcac
     1321 ggcgcggcgc cagcttcgat ggatcgcctg ggggacggtg ctcggggccc ttcccttcgc
     1381 gctgggctac gcgcttccct tcgcgctcgg cgtgcggccg acgctgtcga tggagctgtt
     1441 cgccgtcccg ctcggcctga ttcccctcgc cttcgcgtcc gcgatcgtcc gctaccggct
     1501 gatggacgtg gaggtgatcg tcaagcgttc gatggtctgg gcggcggcca tcgcggcgat
     1561 ctcggcaacc tacgccatcc tcctgcagct ggtcacctgg gcgtttctgc ccaactcgcg
     1621 cgccccgaac acgatcctcg ccgtcctggc gacgctcgtc gtggtgctgc tgtcgcggcc
     1681 gctgaagaac gccatccaga cgatgttgga caaggcgttc taccgcgacc ggtacgacta
     1741 ccgccgcgcg ctcgtcggct tcgcccgcga cctgagctcg gacctcgacc tggcccgcct
     1801 gagcggccgg atcgtcggcc gcgtcaccga gacgctgctg gtggatcgca tggccgtgct
     1861 gctgtgggac gagcgggctg gcgagttcac ctcggtgcgc acggcggggt tgggagggga
     1921 gatcgtccgg ctgccgcggc agggcaccct cggggcgcgg ctggccgccg ggcacaccgt
     1981 ggacctcgac gacgcccgct cgacccgggg gatcgaggcc gatgagatcg acgggtggcg
     2041 cgagcgggag ctgtactact tcatcccgtg tgtgtcgaag gacgcggcca tcgcggtgct
     2101 ggcgctcggg cgcaaggccc acgccgagcc gctgagcagc gaggacatgg cgctgctcgc
     2161 cgcggtcgcg gggcaggcgg cgaccgcgct cgagaacggg cggctgtacc gccagctcca
     2221 cctcaaggcg gacgaactcg ggcggatgcg ggccttcaac gagagcatcc tcgagtcact
     2281 cgaggtgggc ctcacggtgg tgaacgagaa cgaccaggtg gtgcgctgga accgggcgct
     2341 cgaggcgctg accggcgtca gccgcgagca ggcgatcggc cgcccgctgc gcgagctgtt
     2401 ccacccggcc ttctacgatc cgctgcggcg ggcgcgcgac cagtcgccgc gcggcgccag
     2461 cctgtaccgc gtgtcgcttc cggtcggagc cgagggcggc ggctcgcgga ggctggtgaa
     2521 cgtgaccacc gtcccactgc agccgggcga ggggaacccc gccaacggca cggtgatcct
     2581 cgtcgaggac gtgtcggccc gggtgcagct cgaggagcag gtccagatct cggagaagat
     2641 ggcgtcgatc gggctgctgg ccgccggcgt ggcgcacgag gtgaacaccc cgctcaccgg
     2701 catctcgagc ttcacgcaga tgttgctcga gggggccgac ccgggcgacc cgaagacgcg
     2761 cgtgctcgag aagatcgagc ggcagacctt ccgcgcggcg aagatcgtga acggcctgct
     2821 gaacctgtcg cggccgggcg ccgcgtcggc cgagcgcgcc ccggtcgatc tcaacgtggt
     2881 gatcaacgac gtgctgtcgc tgctcgagca ccagttccgc gtgggccaca tccaggtccg
     2941 gcgcgaactg gcctcgccgg ggccggtggt gctcggcatc gagcacaagc tgcagcaggt
     3001 gttcctgaac ctgttcctga acgcccgcga cgcgatgccc aaggggggct ggctgtcgat
     3061 cgcgacgcga gtcgccgacg accaggccgt cgcggagatt gccgacacgg gcgccggcat
     3121 ccccagcgac cacctggcgc gcatttacga
//
LOCUS       DAOPQX010000408         3088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_34408_length_3088_cov_4.270030, whole genome shotgun sequence.
ACCESSION   DAOPQX010000408 DAOPQX010000000
VERSION     DAOPQX010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34408_length_3088_cov_4.270030"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PKK95_15890"
     CDS             <1..377
                     /locus_tag="PKK95_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19751.1"
                     /translation="DIATKLRKYYGANVIGLDFLELDETPGVGAPDSMFWSYGAKILA
                     AAAFARAYPRLHIIYITNFKCGPDSYVKHYVAQVSGRPFLSLQFDGHNNDAGMLTRCE
                     AYLDSKGVLRRWDVRERRAIAS"
     gene            341..1669
                     /locus_tag="PKK95_15895"
     CDS             341..1669
                     /locus_tag="PKK95_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19752.1"
                     /translation="MGRAGTTRDRELNQRKVWVPQMGRGASVLFAAAFRSVGVDAEVC
                     PDSNAETLELGGRHTSGEECLPARVTLGTFLQVARQPGFDPAKAALFMPSADGPCRFG
                     QYAPYLRKVLADVGLGEVMVLSPSSRDGYDALGEKKLELQRTGLRALVAADLLLKMRH
                     RYRPYETVRGATDACYWRAVERVERVVERPGLGTRARIEAIAAALAEGRDEFRRLPVR
                     FTRRRPLIGVVGEIFCRLTPFTNDSLIRKVEEAGGECTLAPLVEWLWYTNREQQKRLR
                     QAGKTLSLSMAAAKLKNRIQERDEHRLYAVFHDDLVGYEEPSVERVLEYSQPYLPHTG
                     SLGEMTLSVGKAVHHYYQGCDGIIDISPFSCMNGIVTEAVYPRVSADHDGIPIRNFFF
                     DGTQTHLARDVAIFMELARSYGARKKVARVYPAGFPPQGEAPPAAAPAVA"
     gene            1690..2688
                     /locus_tag="PKK95_15900"
     CDS             1690..2688
                     /locus_tag="PKK95_15900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOC19753.1"
                     /translation="MKRLPLATSLQIIARGTANWVTRRPIVVSFEVTDSCTCWCRHCD
                     HGGPRDQSKDLQPADYRRYMAALKPCVVQLSGGEPLLRPDIVEVARSIKGGRRVPYLI
                     LVSNWSEMTEEKYLALREAGVNQFSVSLDFPDERHDDFRQLDGLYRHLETIIPRLARL
                     GHDDIVLNTCITSWNVDQVNRAADKAREWGVNISFSAYSPRRINDRRYFIETPRQLEV
                     LRRELDGIKRRMNGTHWIANSCTTLDATRQYFENGGMPNCKSGLRFLVVTSDGALQPC
                     SMQFQRYALEDRARMAREFTATNTCDQCYVSIRSYLDKPFLQLLRENVGAFLGVRS"
     gene            2754..>3088
                     /locus_tag="PKK95_15905"
     CDS             2754..>3088
                     /locus_tag="PKK95_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOC19754.1"
                     /translation="MKVAIIGAGNVGATLAYTLMVRDVASRIGLIDINPEKARGEALD
                     LAHGASYVDNIDLRPEGFEGVADADVIVVTAGRGRRPGESRLDLAKGNVEILEEIIGD
                     ILPYYRGAVV"
BASE COUNT          493 a         1074 c         1078 g          443 t
ORIGIN      
        1 tcgacatcgc gaccaagctg cggaagtact acggcgccaa cgtcatcggc ctcgatttcc
       61 tcgagctcga cgagacgccg ggcgtcggcg cgcccgacag catgttctgg agctacggcg
      121 cgaagatcct cgcggcggcc gcgttcgccc gggcgtaccc gcgcctgcac atcatctaca
      181 tcaccaactt caagtgcggt ccagattcgt acgtgaagca ctacgtggcc caggtgtcgg
      241 ggcggccgtt cctgtcgctg cagttcgacg ggcacaacaa cgacgcggga atgctgacgc
      301 gctgcgaggc gtacctcgac agcaagggag tgctgcgacg atgggacgtg cgggaacgac
      361 gcgcgatcgc gagctgaacc agcggaaggt ctgggtgccg caaatgggac gcggcgcctc
      421 ggtcctcttc gcggcggcct tccggagcgt cggggtggat gcggaggtct gccccgactc
      481 gaacgccgag accctggagc tcggcggccg tcatacctcc ggcgaggaat gcctgcccgc
      541 gcgggtgacg ctcggcacct tcctccaggt ggcccggcag ccggggttcg atcccgcgaa
      601 ggcggccctg ttcatgccca gcgccgacgg cccgtgccgc ttcggccagt acgcgccgta
      661 cctgcgcaag gtgctcgccg acgtcggcct cggcgaggtc atggtgctct cgccgagcag
      721 ccgcgacggc tacgacgccc tcggcgagaa gaagctcgaa ctgcagcgca ccggcctgcg
      781 ggcgctggtt gccgccgacc tgctgctcaa gatgcggcac cgctaccggc cctacgagac
      841 cgtgcggggc gccaccgacg cctgctactg gcgcgcggtg gagcgggtcg aacgggtcgt
      901 cgagcggccg gggctcggca ccagggcgcg catcgaggcc atcgccgcgg cgcttgccga
      961 ggggcgcgac gagttccgcc gcctgcccgt gcggttcacc cgccgccgtc ccctcatcgg
     1021 cgtggtgggc gagatcttct gccgcctgac gcccttcacc aacgactcgc tgatccggaa
     1081 ggtggaggag gcgggcgggg agtgcacgct ggcgccgctg gtcgagtggc tctggtacac
     1141 caaccgcgag cagcagaagc ggctgcgcca ggcgggcaag accttgtcgc tgagcatggc
     1201 cgccgcgaag ctcaagaacc gcatccagga gcgcgacgag caccggctct acgcggtgtt
     1261 ccacgacgac ctggtgggct acgaggagcc gtcggtcgag cgcgtcctcg agtactcgca
     1321 gccctacctg ccccacaccg gctcgctcgg cgagatgacg ctgagcgtcg ggaaggccgt
     1381 gcaccactac taccaggggt gcgacgggat catcgacatc tcgccgttca gctgcatgaa
     1441 cgggatcgtc accgaggcgg tgtacccacg ggtgtcggcc gaccacgacg gcatcccgat
     1501 ccgcaacttc ttcttcgacg gcacgcagac gcacctggcc cgcgacgtcg cgatcttcat
     1561 ggagctggcg cgcagctacg gggcgcgcaa gaaggtggcg cgggtctacc cggccggctt
     1621 tccgccccag ggcgaggcgc cgcccgcggc agcgccggcc gtcgcgtagg cccccggggg
     1681 accacgaaca tgaagaggct tccgctcgcc acctcgctgc agatcatcgc ccgcggcacc
     1741 gcgaactggg tgacccgccg gccgatcgtc gtctccttcg aggtgaccga ctcgtgcacc
     1801 tgctggtgcc ggcactgcga ccacggcggc ccgcgcgacc agtcgaagga cctgcagccg
     1861 gccgactacc gccgctacat ggcggcgctc aagccctgcg tcgtgcagct gtccggcggc
     1921 gagccgctgc tgcgcccgga catcgtcgag gtggcccgct cgatcaaggg cgggaggcgg
     1981 gtgccgtacc tcatcctcgt gtccaactgg tcggagatga ccgaggagaa gtacctggcg
     2041 ctgcgcgagg cgggggtcaa ccagttctcg gtgagcctcg acttccccga cgagcggcac
     2101 gacgacttcc ggcagctcga cggcctgtac cgccacctcg agacgatcat cccgcgcctc
     2161 gcccggctcg gccacgacga catcgttctc aacacctgca tcaccagctg gaacgtggac
     2221 caggtgaaca gggcggccga caaggcgcgc gagtggggcg tcaacatcag cttcagcgcc
     2281 tactcgccgc ggcggatcaa cgaccggcgg tatttcatcg agacgccccg gcagctcgag
     2341 gtcctccgac gggagctgga cgggatcaag cggcgcatga acggcacgca ctggatcgcg
     2401 aactcgtgca cgacgctcga cgcgacgcgc cagtacttcg agaacggggg aatgccgaac
     2461 tgcaagtcgg ggctgcgctt cctcgtggtc acctcggacg gcgccctgca gccgtgctcg
     2521 atgcagttcc agcgttacgc gctcgaggac cgcgcgcgga tggcccgcga gttcaccgcg
     2581 accaacacgt gcgaccagtg ctacgtgtcg atccgctctt acctcgacaa gccgttcctg
     2641 cagctgctgc gcgagaacgt cggggcgttc ctgggagtgc gatcctgacg gcgtaggggc
     2701 cagcgctcag ggctccaggc cgccgggcgg gccgggtgct tcaaggaggc tgcttgaagg
     2761 tcgcgatcat cggcgccggg aacgtggggg cgaccctcgc ctacaccctg atggtcaggg
     2821 acgtggcgag ccggatcggc ctgatcgaca tcaaccccga gaaggcgcga ggcgaggcgc
     2881 tcgacctcgc gcacggcgcc tcgtacgtgg acaacatcga cctgcgcccc gaggggttcg
     2941 agggggttgc cgacgccgac gtgatcgtcg tcacggccgg gcgcggccgg cgcccgggcg
     3001 agtcgcgcct cgacctggcc aaggggaacg tcgagatcct cgaggagatc atcggcgaca
     3061 tcctgccgta ctaccgcggc gcggtggt
//
LOCUS       DAOPQX010000409         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_34461_length_3084_cov_4.697260, whole genome shotgun sequence.
ACCESSION   DAOPQX010000409 DAOPQX010000000
VERSION     DAOPQX010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34461_length_3084_cov_4.697260"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKK95_15910"
     CDS             <1..601
                     /locus_tag="PKK95_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19755.1"
                     /translation="YEAWLNGVRSAGDVARDAFVECPIVHPTLMMRRRMAALGYEDRG
                     WPEDYDLVLRALAAGLRIGVVPRRLLAWRDRPDRLSRADGRYSRTMFTACKAHYLAAG
                     FLAGGERYVLWGYGGTGQALYRALAALGKRPSNIVEVKPGRIGKRIHGAMVIRPEALP
                     AIERARLVVSVAHAGPRAEIRGILATLGWVEGRDFRCAA"
     gene            complement(620..2071)
                     /locus_tag="PKK95_15915"
     CDS             complement(620..2071)
                     /locus_tag="PKK95_15915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOC19756.1"
                     /translation="MAKRNVSRLAVIAIFSALAAGACATNPVTGKRELALISESQEIA
                     MGRDADKEIVAAYGLYPDDRLQQYLQELGSRIAAKTERPALPWTFRVVDDAAVNAFAL
                     PGGFIYVTRGIMTHLNSEAELVTILGHEIGHVTARHSVNQMSKQQLAQIGLVAGMVLS
                     PRVAQFGNLAQTGLGILFLSFSRADESQSDELALKYMTAAGYDPREMVDVFRMLEGVS
                     QQAGGGRLPQWLSSHPNPENREAWAARAAASVKSDPSRMAVNREGYLRRVDGMVFGDN
                     PREGFFQQSAFRHPDLAFRINFPSGWKGSNQKQAVGAVSPSEDAMIVLQLAGVPSAQQ
                     AARQFFSQEGVQAGQAWRQQIGGFPAVSYVFRAQAEQTIVQGLVAWIEYGNRVYQVMG
                     YTPEQRWGAYDDAIAQSIGSFARETDRSVLEVQPNRVRLVEVDRPATLETLVSRYPSA
                     VPPQVLGLINDLQGNQTLPAGSLFKRVVNTTPR"
     gene            complement(2223..2828)
                     /locus_tag="PKK95_15920"
     CDS             complement(2223..2828)
                     /locus_tag="PKK95_15920"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HOC19757.1"
                     /translation="MFKRSVAAAAVLLAVVGLPSTARAHCDTLDGPVVKAARVALERG
                     DVQPVLKWVKADAEPELRAAFEKTLAVRRAGGDSRELADRYFFETLVRLHRAGEGAPY
                     TGLKPAGTTEKPIAAADHALEQGSVDALSRMLAEHLASGVRERFMRVKETAPAADRTP
                     EDGRRYVAAYVEYTHYIEAIARAVHAQGGHAHGEGTEHAGH"
BASE COUNT          419 a         1081 c         1100 g          484 t
ORIGIN      
        1 gtacgaggcg tggttgaacg gcgtgcgctc ggcgggcgac gttgcgcgcg acgcgttcgt
       61 ggagtgcccg atcgtccacc cgacgctgat gatgcgccgt cgaatggcgg ccttggggta
      121 cgaggaccgc ggctggcccg aggactacga cctggtgctg cgggcgctcg ccgcgggcct
      181 ccggatcggt gtggtgcccc gccgcctgct ggcgtggcgc gaccggcccg accggctgtc
      241 gcgcgcggat ggccgctact cgcgcacgat gttcaccgcc tgcaaggcgc actacctggc
      301 cgcggggttc ctcgccgggg gcgagcggta cgtgctgtgg ggatacgggg gcacgggcca
      361 ggcgctgtac cgggcgctgg cggccctcgg caagcggccc tcgaacatcg tcgaggtgaa
      421 gcccgggcgg atcggcaagc ggattcacgg cgcgatggtg atccgtcccg aggcgctgcc
      481 cgcgatcgag cgggcacggc tcgtggtctc ggtggcgcac gccggcccgc gcgcagagat
      541 ccgcgggatc ctcgcgaccc ttgggtgggt cgagggccgc gacttccgct gtgcggcctg
      601 atccctccgg cctccctccc taccgcggcg tcgtgttcac aacccgcttg aacaggctgc
      661 cggccggcag cgtctggttg ccctgcaggt cgttgatgag tccgagcact tgcggcggca
      721 cggcggaggg gtagcggctg acgagcgtct cgagggtggc cgggcggtcc acttccacca
      781 atcggacgcg gttcggttgc acctcgagaa ccgagcggtc ggtctcgcgg gcgaagctgc
      841 cgatcgactg tgcgatggcg tcgtcgtagg cgccccagcg ttgctcgggg gtatacccca
      901 tgacctggta gacccggttg ccgtactcga tccaggcgac gagcccctgg acgatcgtct
      961 gctcggcctg ggctctgaac acgtacgaca cggccgggaa gccgccgatc tgctggcgcc
     1021 acgcctggcc cgcctggacc ccctcctgcg agaagaactg ccgcgccgcc tgctgtgcgc
     1081 tcgggacgcc ggccagttgc aggacgatca tcgcgtcctc gctggggctc accgccccga
     1141 ccgcctgctt ctggttgctg cccttccagc ccgacgggaa gttgatgcgg aaggcgaggt
     1201 cggggtggcg gaacgccgac tgctggaaga acccctcgcg cgggttgtcg ccgaacacca
     1261 tgccgtcgac gcgccgcagg taaccctcgc ggttcaccgc catgcgggag gggtcgctct
     1321 tcaccgacgc cgccgcccgt gcggcccacg cctcgcggtt ctcggggttg gggtggctcg
     1381 agagccactg tggcagccgg ccgccgcccg cctgctggct gaccccctcg agcatgcgga
     1441 agacgtccac catctcgcgc ggatcgtaac cggccgcggt catgtacttg agcgccagct
     1501 cgtccgactg cgactcgtcc gcgcggctga acgacaggaa gagaatgccg aggcccgtct
     1561 gtgcgaggtt tccgaactgg gccacacgcg gcgacagcac catgccggcc accagcccga
     1621 tctgcgccag ctgctgcttg ctcatctggt tgaccgagtg gcgggcggtg acgtgcccga
     1681 tctcgtggcc gaggatcgtc accagctcgg cctccgagtt gaggtgggtc atgatgccgc
     1741 gcgtgacgta gatgaagccg ccgggcagcg cgaacgcgtt gacggcggcg tcgtccacga
     1801 cccggaaggt ccagggcagg gcggggcgct cggtcttggc ggcaatgcgg gagcccagct
     1861 cctgcaagta ctgctgcagg cggtcgtcag gatagagccc gtacgccgca acgatctcct
     1921 tgtcggcgtc gcggcccatc gcgatttcct gcgactcgct gatcagcgcg agctcgcgct
     1981 tgccggtgac agggttggtg gcgcacgcac cggcggccag ggccgagaag atggcgatca
     2041 ccgcaagccg cgagacgttc cgctttgcca tggctgtccc cccgcggacc attgtatgcc
     2101 ttctcagctg tatgggcgcc cggtggtggc agggccgctc accgtgcgcc agaagcgcac
     2161 ggtgaggtag gaggccgttc gaagacgaga cccgagagac aggttccgac ctggcagccg
     2221 gctcagtggc cggcgtgctc cgtgccttcg ccgtgcgcgt gtccgccttg cgcgtgcacg
     2281 gcccgcgcga tcgcctcgat atagtgcgtg tactccacgt acgccgcgac gtaccggcgg
     2341 ccgtcctcgg gggtccggtc ggcggccggc gcggtttcct tcacccgcat gaaccgctcg
     2401 cggaccccgc tggccaggtg ctcggcgagc atgcggctga gggcatcgac cgagccctgc
     2461 tcgagcgcgt ggtcggcggc ggcgattggc ttctcggtgg tgccggccgg cttgaggccg
     2521 gtgtaggggg ccccttcacc cgcccgatgg agccggacga gggtttcgaa gaaataccga
     2581 tcggccagct cgcgcgagtc gccgcccgcc cgccggacgg ccagggtctt ctcgaacgcc
     2641 gcccgcagct cgggctcggc gtcagccttc acccacttca ataccggctg cacgtcgccc
     2701 cgctcgagcg ccactcgcgc cgccttcacc acgggaccat cgagggtgtc gcagtgcgct
     2761 cgcgccgtcg aaggaagccc caccaccgcc aacagcaccg cggccgccgc gaccgatcgc
     2821 ttgaacatct gaacctcctc ggtcagcgta gcccgttcgc ccctccggcc gttatgacgc
     2881 aggtcacggc cgccggtata tcatccgtgc ggctcgacgg gccgcgatag gtgttcccca
     2941 tgcaacccgg cgatcgtggc gggcgagagc gccgctacgc cagccttgtc gggttgcagg
     3001 accggtcagc tgacaggcaa cgcggccccc tgcgaacgcg cccgcccgtc agcaggagtg
     3061 cggcaggagc ctccggaggt cgac
//
LOCUS       DAOPQX010000410         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_34804_length_3062_cov_3.806119, whole genome shotgun sequence.
ACCESSION   DAOPQX010000410 DAOPQX010000000
VERSION     DAOPQX010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34804_length_3062_cov_3.806119"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..301)
                     /locus_tag="PKK95_15925"
     CDS             complement(101..301)
                     /locus_tag="PKK95_15925"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOC19758.1"
                     /translation="MRDCLERLVGEMVERGIRFEDALGVFERTFIAAVLARTDGNLGE
                     AARVLRIHRNTLRRKMGEYKLR"
     gene            complement(322..1230)
                     /locus_tag="PKK95_15930"
     CDS             complement(322..1230)
                     /locus_tag="PKK95_15930"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOC19759.1"
                     /translation="MNLPAALLSTSVPGAPEPRRGKVRDVYEAGDDLLIVATDRISAF
                     DYVLGTGIPDKGKVLTALSAFWFDLTAPLLPNHVITTDVSRFPAPFDAHPDVLAGRSM
                     LVRRTRPVPIECVVRGYLVGSGWAEYKRTGQVCGITLPAGLREADRLPEPIFTPSTKA
                     EAGHDVNISEEEAGRLVGTALIGRLKELSLQVYRTGAAHAESRGIIVADTKFEFGLAG
                     GPGRDEVLLIDEVLTPDSSRFWPKDAYRPGGSQPSFDKQYVRDYLEQIKWNKQPPVPS
                     LPGEVVARTREKYLEALRRLSGRELA"
     gene            1354..2385
                     /locus_tag="PKK95_15935"
     CDS             1354..2385
                     /locus_tag="PKK95_15935"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOC19760.1"
                     /translation="MRAHLRTVVVVGLAVALLAWFLRNADLASVAGELGRGRPWLLAL
                     ALAATLTTYATRSLRWQYLLRGVGPTRYVNALRATVIGFAASFLLPARAGELVRPYLL
                     AQREGLPFTATFATVILERVLDLVTVLILFSAYLLLAGPGAGEASPAMFRAVKVGGIA
                     AAAATTVVLAVFFFLAGRPEALGRAALRIERVLPARLAHLVARLAQAFAEGLAAVRNP
                     QHLAMVLFWSFPLWLSIAAGIWLVTRAFHLAIPYPASFLIMTVLVVGVAVPTPGAVGG
                     FHEAYRFTVTTFFAAPNDRAVGAALVLHAISFVPVTLVGIVFMAQEGLTLGRVRVLAR
                     RAGAKEDEG"
     gene            2382..2840
                     /gene="nrdR"
                     /locus_tag="PKK95_15940"
     CDS             2382..2840
                     /gene="nrdR"
                     /locus_tag="PKK95_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOC19761.1"
                     /translation="MKCPYCGHLGDKVVDSRESKEGDAIRRRRECLVCGRRFTSYERI
                     DEIPYMVVKKDGSRERFERQKLIAGMLKACEKRPVSVSALEKVADRVEATLQERPERE
                     ISTTEIGQFVMQALKGLDAVAYVRFASVYRNFRDVGEFKKQLEELLKTSE"
     gene            2837..>3062
                     /locus_tag="PKK95_15945"
     CDS             2837..>3062
                     /locus_tag="PKK95_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19762.1"
                     /translation="MIRRALLAALAAAVLGACSGPPIGGVYALSGAAAGDEGLRVASL
                     AKDGQLLVSFSLADGLTRELRQAIDSGLPAT"
BASE COUNT          406 a         1044 c         1119 g          493 t
ORIGIN      
        1 cacgagaatc gagtgctaat tctaggtccc cccgcggagc ggtgtcaagg gggtgggacg
       61 gcgatgggta ggagcggcga acgagggccg gggcgtagaa ctacctgagc ttgtactcgc
      121 ccatcttcct ccgcagcgtg ttccggtgga tgcgcagcac gcgggccgct tcacccaggt
      181 tgccgtcggt ccgggcgagg accgccgcga tgaaggtccg ctcgaagacc ccgagggcgt
      241 cctcaaaccg gatcccccgt tccaccatct cgccgacgag ccgctcgaga cagtcccgca
      301 ccgtgcctcc cgccctgcgg cctacgccag ctcccggccc gacagccggc gcagcgcctc
      361 gaggtacttc tcccgggtgc gcgccaccac ctcgccgggc agcgacggga ccggcggctg
      421 cttgttccac ttgatctgct cgaggtaatc acggacgtac tgcttgtcga agctgggctg
      481 gctgccgccg gggcggtagg cgtccttcgg ccagaagcgc gaggagtcgg gggtgagcac
      541 ctcgtcgatg agcagcacct cgtcacggcc gggcccgcca gccaggccga actcgaactt
      601 ggtgtcggcg acgatgatgc cccgcgactc ggcgtgcgcg gcgccggtcc ggtagacctg
      661 cagcgacagc tccttcaggc gtccgatgag ggccgtcccg accagccgtc cggcctcttc
      721 ctcggagatg ttgacgtcgt ggccggcctc ggccttggtg gacggggtga agatcggttc
      781 cggcaggcga tcggcttccc gcagccccgc cggcagcgtg atcccgcaca cctgtcccgt
      841 cctcttgtac tcggcccacc ccgatcccac caggtagccg cgcaccacgc actcgatcgg
      901 gaccggccgc gtgcgccgga ccagcatcga ccgcccggcc agcacgtcgg ggtgggcgtc
      961 gaagggcgcc gggaacctcg acacgtccgt ggtgatgacg tgattgggca ggagcggcgc
     1021 ggtgaggtcg aaccagaatg cggagagcgc ggtcagcacc ttgcccttgt cgggaatgcc
     1081 ggtgccgagc acgtagtcga acgcggagat ccggtcggta gcgacgatga gcaggtcgtc
     1141 gcccgcctcg tagacgtcgc gcaccttccc ccggcgcggc tccggggctc cgggaaccga
     1201 cgtggacagc agggcagccg gcaggttcat cgggtgacct cggggatgcg agagaagaag
     1261 ctcatcttac catcaccgtt ccccatttgc cttgctccag ttcccctcag ccttccgcgc
     1321 cccgtccttt tatagtagag tccattcgct cggatgcgcg cgcacctcag gacggtcgtg
     1381 gtcgtcggcc tcgcggtggc cctgctcgcc tggttcctcc gcaacgccga cctcgcgtcg
     1441 gtggccggcg agctggggcg cggcaggccg tggctgctgg cgctcgcgct ggccgcgacg
     1501 ctgacgacgt acgcgacgcg aagcctgcgg tggcagtacc ttctgcgggg agtggggccg
     1561 acccggtacg tgaatgcgct tcgcgcgacg gtgatcgggt tcgcggccag cttcctgctg
     1621 ccggcgcggg ctggcgagct ggtgcgcccc tacctgttgg cgcagcgcga ggggttgccc
     1681 ttcacggcca ccttcgccac cgtgatcctc gagcgggtgc tcgacctggt gacggtgctg
     1741 atcctcttca gcgcctacct gctgctggca ggcccggggg cgggggaggc cagcccggcg
     1801 atgttccggg cggtgaaggt cggcgggatt gccgccgccg cggcgacgac cgtcgtgctg
     1861 gccgtgttct tcttcctggc gggccggccc gaggcgctcg ggcgcgcggc gctccggatc
     1921 gagcgggtgt tgccggcgag gctcgctcac ctggtggcgc gcctggcgca ggccttcgcc
     1981 gaggggctgg cagccgtccg gaacccgcag cacctggcga tggtcctgtt ctggtcgttc
     2041 ccgctgtggc tgtcgatcgc ggcgggcatc tggctggtga cgcgcgcgtt ccacctggcg
     2101 atcccgtacc cggcgtcgtt cctgatcatg acggtgctgg tggtgggcgt ggcggtgccg
     2161 acgccgggcg ccgtgggggg gttccacgag gcctaccgct tcacggtcac gactttcttc
     2221 gcggcgccca acgatcgggc ggtgggtgcc gcgctcgtcc tgcacgcgat ctcgttcgtg
     2281 ccggtgacgc tggtggggat cgtgttcatg gcgcaggagg ggctgacgct cgggcgcgtg
     2341 cgcgtcctgg cccggcgggc cggggcgaag gaggacgagg ggtgaagtgc ccgtactgcg
     2401 gtcacctggg cgacaaggtc gtcgactcgc gcgagagcaa ggagggcgac gcgatccgcc
     2461 gccggcggga gtgcctcgtc tgcggccgcc gcttcacctc ctacgagcgg atcgacgaga
     2521 ttccctacat ggtcgtcaag aaggacggca gccgcgagcg cttcgagcgg cagaagctga
     2581 tcgccggcat gctgaaggcc tgcgagaaga ggcccgtcag cgtgtcggcg ctcgagaagg
     2641 tggccgaccg cgtggaggcc accctgcagg agcggcccga gcgcgagatc tcgaccaccg
     2701 agatcggtca gttcgtcatg caggcgctga aggggctcga cgccgtcgca tacgtgcggt
     2761 tcgcgtcggt ctaccggaac ttccgggacg tgggcgagtt caagaagcag ctcgaggagc
     2821 tgctgaagac atccgaatga tccgacgggc gctactggcc gcgctggcgg ccgccgtgct
     2881 cggcgcctgc tccggccccc ccatcggcgg cgtctacgcc ctctcgggcg cggcggccgg
     2941 cgacgagggg ctgcgcgtcg cctcgctcgc caaggacggg cagctcctgg tgtcgttctc
     3001 gctggccgac ggcctcaccc gggaactgcg ccaggcgatc gacagcggcc tgccggcgac
     3061 ca
//
LOCUS       DAOPQX010000411         3053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_34923_length_3053_cov_4.843562, whole genome shotgun sequence.
ACCESSION   DAOPQX010000411 DAOPQX010000000
VERSION     DAOPQX010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34923_length_3053_cov_4.843562"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="PKK95_15950"
     CDS             <1..1569
                     /locus_tag="PKK95_15950"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOC19763.1"
                     /translation="EGHGYRVTLQKFDPYINVDPGTMSPFQHGEVYVTDDGAETDLDL
                     GHYERFTSTVTTRNHNWTTGKIYLSVIQKERRGDYLGRTVQVIPHITNEIKECLRAAG
                     KDVHVVLVEIGGTVGDIESQPFLEAIRQFRQDVGRDNTLYIHLTLVPFIGTAGELKTK
                     PTQHSVRDLRSMGIQPDILLCRTDRFLPQDIKRKIALFCDVSEEAVIASRDVSSIYEV
                     PLSLAAEGVDSIILQYLRLPQTQLHVEQWQRLVERIKHPADEVTVHVVGKYVEYEDSY
                     KSLNEAMLHGGFAHGLKVNFQWVEAEALEEPDGEKLLDAADGILVPGGFGTRGTRGMM
                     KAAEVARTRQVPFFGICYGFQWATVEFARNVCGLEGADSTEVNEAAPHKVIYKLRDLL
                     GVDDLGGTMRLGQYRCDLVPGSLAHRVYGADVIFERHRHRYEFNRAYEGALTERGLLI
                     SGRSPDGKFVEIAELPGHPWFIAVQFHPEFKSKPLRPHPLFAGFIEASHRHRQERLAA
                     SSQRAEAGFQHAAE"
     gene            1618..2460
                     /gene="kdsA"
                     /locus_tag="PKK95_15955"
     CDS             1618..2460
                     /gene="kdsA"
                     /locus_tag="PKK95_15955"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOC19764.1"
                     /translation="MKSVAIGPVRIGAGPLTLIAGPCVIEGEAHALGLAGRLRDIAAS
                     RGVPFVFKASFDKANRTSVTSYRGPGLEEGLRILSRVKSEVAVPVLTDIHEAWQADAA
                     AQVADVLQIPAFLCRQTDLLLAAGRTGRAVNIKKGQFLAPRDVRHAVAKVVSTGNDQV
                     LVTERGTSFGYNNLVVDVRAFPMLREIGYPVVFDVTHSLQLPGAGDGVTAGLAEYIEP
                     LASAGVAAGVDGVFLEVHEEPARARSDAQNALRLDALGPLLDRLLRIDAAVRETAGST
                     RGHQ"
     gene            2447..>3053
                     /locus_tag="PKK95_15960"
     CDS             2447..>3053
                     /locus_tag="PKK95_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOC19765.1"
                     /translation="MVTSDAMTDLELARKVLETEAAAILRLVERLDERFEQAVTLLQE
                     CRGRAILTGMGKSGIICRKIAATLASTGTPAFFLHPAEAIHGDLGVLQAEDAVVMVSY
                     SGETGELVRLLELIRRVGARIIAITGAPGSTLAQAADIALDCRVSEEACPLNLAPTAS
                     TTAALALGDALAMAISVRKGFREEDFANLHPGGKLGRRLLRI"
BASE COUNT          488 a         1068 c         1061 g          436 t
ORIGIN      
        1 gaagggcacg gctaccgcgt gacgctccag aagttcgacc cctacatcaa cgtcgatccg
       61 ggcaccatga gcccgttcca gcacggcgag gtctacgtca ccgacgacgg ggctgagacc
      121 gacctcgacc tgggccacta cgagcgcttc acgagcaccg tcaccacgcg caaccacaac
      181 tggacgaccg gcaagatcta cctgagcgtc atccagaagg agcggcgcgg cgactacctg
      241 ggccgcaccg tccaggtgat cccccacatc accaacgaga tcaaggagtg cctccgcgcc
      301 gccgggaagg acgtgcacgt cgtgctggtg gagatcggcg gcacggtcgg cgacatcgag
      361 agccaaccct tcctcgaagc cattcggcag ttccggcagg acgtcggccg cgacaacact
      421 ctgtacatcc acctcacgct cgtgcccttc atcggcacgg cgggcgagct gaagaccaag
      481 ccgacccagc acagcgtgcg cgacctgcgc tcgatgggga tccagcccga catcctgctc
      541 tgccgcaccg accggttcct gccccaggac atcaagcgca agatcgcgct gttctgcgac
      601 gtcagcgagg aggcggtcat cgcctcgcgc gacgtgtcgt cgatctacga ggtgcccctc
      661 agccttgccg cagagggtgt ggactcgatc atcctgcagt acctccggct gccccagacc
      721 cagctgcacg tcgagcagtg gcagcgtctc gtcgagcgga tcaagcaccc tgccgacgag
      781 gtgaccgtcc acgtggtggg caagtacgtc gagtacgagg actcctacaa gagcctgaac
      841 gaggcgatgc tgcacggcgg cttcgcgcac gggctgaagg tgaacttcca gtgggtggag
      901 gccgaggccc tcgaggagcc ggatggtgag aagctgctcg acgcggccga cggaatcctc
      961 gtccccggcg ggttcggcac gcgcggcacg cgcgggatga tgaaggccgc cgaggtcgca
     1021 cgcacccggc aggtcccgtt cttcggcatc tgctacggct tccagtgggc aaccgtcgag
     1081 ttcgcgcgga acgtgtgcgg cctcgagggc gccgactcga ccgaggtcaa cgaggccgcc
     1141 ccgcacaagg tgatctacaa gctgcgagac ctgctgggcg tcgacgacct gggcgggacg
     1201 atgaggctcg ggcagtaccg gtgcgacctg gtgcccgggt cgctggctca ccgcgtctac
     1261 ggcgccgacg tgatcttcga gcgccaccgg caccgctacg agttcaaccg ggcctacgag
     1321 ggggcgctca ccgagcgcgg gctgctgatc tcgggccggt cgcccgacgg gaagttcgtg
     1381 gagattgccg agctgccggg ccacccctgg ttcatcgcgg tgcagttcca ccccgagttc
     1441 aagtcgaagc cgctgcgccc ccacccgctg ttcgccgggt tcatcgaagc cagccaccgc
     1501 caccggcagg aacggctcgc cgcctcgagc cagcgggcgg aggcgggctt ccagcacgcc
     1561 gccgagtagt gcggcggtgg agcggtgcgc cgctgacctt cacgtataat ggcccgcgtg
     1621 aagagcgtgg ccatcggccc agtccggatt ggcgcggggc cgctgacgct gattgccggg
     1681 ccctgcgtca tcgagggcga ggcccacgcg ctcggccttg ccggccggct gcgcgacatc
     1741 gcggcgtcgc gcggcgtgcc gttcgtcttc aaggcctcct tcgacaaggc gaaccggacg
     1801 tcggtgacct cgtatcgcgg ccccggcctc gaggaagggc tgcgcatcct gtcgcgcgtg
     1861 aagagcgagg tcgcggtgcc ggtcctcacc gacatccacg aggcgtggca ggctgacgcg
     1921 gccgcgcagg tggcggacgt tctccagatc ccggcgttcc tctgccggca aaccgacctg
     1981 ctgctcgctg cggggcggac ggggcgcgcg gtgaacatca agaaggggca gttcctcgcg
     2041 ccgcgcgacg tgcgtcacgc cgtagccaag gtggtgtcca cggggaacga ccaggtgctg
     2101 gtgaccgagc ggggcacctc gttcggctac aacaacctgg tcgtggacgt gcgtgcgttc
     2161 cccatgctgc gggagatcgg ctacccggtc gtcttcgacg tgacccacag cctccagctg
     2221 cccggcgcgg gcgacggggt gacggccggg ctggcagagt acatcgagcc gctcgcctcg
     2281 gcaggagtgg cggcgggagt ggacggcgtc ttcctcgagg tgcacgagga gcccgcgcgc
     2341 gcgcggagcg acgcgcagaa cgcgctgcgg ctcgacgccc tcggcccact cctcgaccgc
     2401 ctgctgcgga tcgacgccgc cgtccgcgag acggccggga gcacgcgtgg tcaccagtga
     2461 cgcgatgacc gatctcgagc tggcacgcaa ggtcctcgag accgaggcgg ccgccatcct
     2521 ccgcctcgtg gagcgcctcg acgagcggtt cgagcaagcg gtaacgctgc tgcaggagtg
     2581 ccgcggccgg gcgatcctca ccggcatggg caagtcgggc atcatctgcc ggaagatcgc
     2641 ggccacgctc gccagcaccg gcacgccggc cttcttcctc catcccgccg aggccattca
     2701 cggcgacctc ggcgtcctgc aggccgagga cgccgtcgtg atggtgtcct acagcggcga
     2761 gaccggcgag ctggtccggc tgctggagct gatccggcgg gtcggtgcgc gcatcatcgc
     2821 gatcaccgga gcccccgggt cgacgctggc gcaggcagcc gacatcgcgc tcgactgccg
     2881 ggtgagcgag gaggcctgcc ctctgaacct cgccccgacc gcgagcacca ccgccgccct
     2941 cgcgctcggc gacgcgctgg cgatggcgat ctcggtgcgc aagggtttcc gcgaggagga
     3001 cttcgccaac ctgcacccgg gcggcaagct gggacgtcgc ctgctgcgga tcg
//
LOCUS       DAOPQX010000412         3048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_34994_length_3048_cov_3.816572, whole genome shotgun sequence.
ACCESSION   DAOPQX010000412 DAOPQX010000000
VERSION     DAOPQX010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34994_length_3048_cov_3.816572"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKK95_15965"
     CDS             complement(<1..606)
                     /locus_tag="PKK95_15965"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOC19766.1"
                     /translation="MKRPRMAGPKPCLTLVACGLALAVAGCSGPREPAPAASPLPRHL
                     VLVTIDTLRADRVGAYGYGGARTPVLDAIARGGVRFERAFAPAPITLTSHASLLTGLY
                     PPGHGARHNGMRLKAGTPTLAASLRAQGFATGAFVAAFPLERRFGLDAGFDVYGDRLP
                     RGPGGRPGVERPGREVVDEAVAWWRASTAARRFLWVHLFEPH"
     gene            complement(718..>3048)
                     /locus_tag="PKK95_15970"
     CDS             complement(718..>3048)
                     /locus_tag="PKK95_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOC19767.1"
                     /translation="NDFHGTAYYFGRNQNLVGNGPNDREFGTFSNKQFGGSLGGPIVK
                     NRAFFFGNGEISRRDTPSGTSIAGSGANFGRTQDVDRFLSILKSKYGYDAGSTDEFTR
                     KTDSDKIFVRADLNVNPRNQLTVRHNYVKGVNDIGSPSVRAYIFPDNFYQFNSKTNST
                     VVQLNSTIGQLFNEFRFTAQRIREFRDRPEYKVFPQVQVTLPDNATLRAGREQFSAAN
                     ALDQDIYELTDDVTLVRGKHTITFGTHNEFYKFRNVFIRDNAGTYSFDSLDLFEQGLA
                     QSYDYSFGALDPNFAARFKVYQFGVYAGDQWRVADRLSLNVGLRVDKPSFPDTPTANP
                     LAVATYGYATDVTPNPTMWSPRVGFNWRPGDNTREQVRGGMGLFSGRTPYVWLSNQYG
                     NTGIEISRLRVSFNRANRIPFVSDPGSQPKVITGGQSVAGEIDMVDPDYNYPQLLRGN
                     LAYDRELGFLGLVGTGEVIYSRNIQDVKYQNLNLTPMGTRPDGRPYYARLAPALGDAI
                     FLTNTDQGDSWSLAFKVDRPWRNGFHASASYLYNQAHSIMDGTSSQAASNWGNVYVPG
                     DPNNPPLTVSNFDVRHRINLSASYQLPLRVVSATLSAFYNGQAGRPYALAFFQDVNGD
                     ARTSNDLLFIPSSASDVIVRNGTGDQLMSWLNADGCAMDFAGAIQERNSCRQPWTHQM
                     DVRLGVNVPFGGTRRVELEFNVFNFLNMLNKDWGKVQSVAYNQNTDVRYGGIDAATGK
                     MIYDIATISSSTYPGRFLVDDVRSRWQAQFGARFRF"
BASE COUNT          465 a          979 c         1096 g          508 t
ORIGIN      
        1 gtgaggctcg aagaggtgaa cccagaggaa ccgccgcgcc gccgtgctgg ctcgccacca
       61 ggcaacggct tcgtccacca cctcgcgccc cggccgctcc acgccgggac ggccgccggg
      121 cccgcgcggc aggcgatcgc cgtagacgtc gaagccggcg tcgaggccga accgccgctc
      181 gagcgggaac gccgccacga atgccccggt ggcgaatccc tgcgcccgca gggaagcggc
      241 cagcgtcggc gtgccggcct tcagacgcat gccgttatgg cgggcgccgt ggccgggcgg
      301 gtagaggccg gtcagcaggc ttgcgtgaga ggtcagcgtg atgggtgccg gcgcgaaggc
      361 ccgctcgaag cgaacgccgc cccgcgcgat cgcgtcgagg accggtgtgc gggcgccgcc
      421 gtacccgtac gcgccgacac gatcggcgcg cagcgtgtcg atggtgacga ggacgaggtg
      481 gcgggggaga ggagacgcgg cgggggcggg ctcgcgcggc ccgctgcagc cggcgaccgc
      541 gagagccagt ccacacgcca cgagggtgag gcagggcttc ggccctgcca tccgcggccg
      601 cttcaccgcg tcattgtagg gccaaagcga agagggcggg acgccaggcc cgaatgcccg
      661 gcgtcccgcc ctcaggctat ccgctgtcgg ctgtcagctg tgcgctgtca gctgctccta
      721 gaaccggaac cgcgcaccga actgcgcctg ccaccgcgac cgcacgtcgt ccaccaggaa
      781 ccgcccgggg taggtggacg agctgatcgt cgcgatgtcg tagatcatct tgccggtggc
      841 ggcgtcgatg ccgccgtagc gcacgtcggt gttctggttg tacgccacgg actgcacctt
      901 gccccagtcc ttgttcagca tgttcaggaa gttgaacacg ttgaactcca gctcgacccg
      961 gcgcgtgccg ccgaacggca cgttcacgcc gaggcggacg tccatctggt gcgtccaggg
     1021 ctggcggcag gagttgcgct cctggatggc gccggcgaag tccattgcgc acccgtcggc
     1081 gttgagccag gacatcagct gatccccggt gccattgcgg acgatgacgt cgctcgcgct
     1141 ggaggggatg aagagcaggt cgttgctggt gcgcgcgtcg ccgttgacgt cctggaagaa
     1201 cgccagcgcg tagggacggc cagcctggcc gttgtagaag gccgacagcg tggccgacac
     1261 cactcgcagc ggcagctggt aggaggcgct caggttgatc cggtgccgca cgtcgaagtt
     1321 cgacacggtc agcggcgggt tgttcgggtc gccgggcacg tacacgttgc cccagttcga
     1381 cgccgcctgc gacgaggtgc cgtccatgat cgagtgagcc tggttgtaga ggtacgaggc
     1441 cgacgcgtgg aagccgttgc gccacgggcg gtccaccttg aacgccaggc tccacgaatc
     1501 gccctggtcg gtgttggtca ggaagatcgc gtcgccgagc gcgggcgcca gccgggcgta
     1561 gtacggccgg ccgtcgggcc gggtgcccat cggcgtcagg ttcaggttct ggtacttcac
     1621 gtcctggatg ttgcgcgagt agatgacctc gcccgtcccg acgagaccca ggaaccccag
     1681 ctcgcggtcg taggccaggt tgccccggag cagctgcggg tagttgtagt cgggatcgac
     1741 catgtcgatc tcacccgcca ccgattgtcc acccgtgatg accttcggct ggctgccggg
     1801 atccgacacg aacgggatcc ggttggcgcg gttgaagctc acgcgcagcc gcgagatctc
     1861 gatgccggtg ttgccgtact ggttggacag ccagacgtac ggggtgcggc ccgagaagag
     1921 gcccatgccg ccgcgcacct gctcgcgcgt gttgtcgccc ggccgccagt tgaagcccac
     1981 ccgcggcgac cacatcgtcg ggttcggcgt cacgtcggtg gcgtacccgt aggtcgcgac
     2041 cgccagcggg ttggccgtcg gcgtgtcggg gaacgacggc ttgtccacgc gcaggccgac
     2101 gttcaggctc aggcggtcgg cgacgcgcca ctggtcaccc gcgtagacgc cgaactggta
     2161 gaccttgaat cgggcggcga agttcgggtc gagggcgccg aagctgtagt cgtacgactg
     2221 ggccagcccc tgctcgaaga gatcgaggct gtcgaacgag taggtgcccg cgttgtcgcg
     2281 gatgaacacg ttgcggaact tgtagaactc gttgtgcgtg ccgaacgtga tggtgtgctt
     2341 gccgcgcacc agcgtcacgt cgtcggtcag ctcgtagatg tcctggtcga gggcgttggc
     2401 cgccgagaac tgctcgcgcc cggcgcgcag cgtggcgttg tcgggcaacg tcacctggac
     2461 ctgcgggaag accttgtact cgggccggtc gcggaactcg cggatgcgct gcgccgtgaa
     2521 gcggaactcg ttgaagagct ggccgatcgt gctgttcagc tgcacgaccg tcgagttggt
     2581 cttcgagttg aactggtaga agttgtccgg gaagatgtac gcccgaacgc tgggcgagcc
     2641 gatgtcgttg acgcccttca cgtagttgtg gcgcaccgtc agctggttgc gcgggttcac
     2701 gttgaggtcg gcccgcacga agatcttgtc gctgtcggtc ttccgggtga attcgtcggt
     2761 gctgcccgcg tcgtacccgt acttgctctt caggatcgag agaaaccggt ccacgtcctg
     2821 ggtccggccg aagttcgcgc ccgacccggc gatcgaggtg cccgacggcg tgtcgcgccg
     2881 tgaaatctcg ccgttgccga agaagaacgc ccggttcttg acgatcggcc cgccgaggct
     2941 gccgccgaac tgcttgttgc tgaacgtccc gaactcgcgg tcgttcgggc cgttgcccac
     3001 caggttctgg ttgcgcccaa agtaatacgc ggtcccgtgg aagtcgtt
//
LOCUS       DAOPQX010000413         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_35155_length_3037_cov_4.043260, whole genome shotgun sequence.
ACCESSION   DAOPQX010000413 DAOPQX010000000
VERSION     DAOPQX010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35155_length_3037_cov_4.043260"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /gene="dxr"
                     /locus_tag="PKK95_15975"
     CDS             <1..1112
                     /gene="dxr"
                     /locus_tag="PKK95_15975"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOC19768.1"
                     /translation="RHRDERRAEALGLLAGSNGALLCEQAERFRAALVSLASASALAA
                     ASAALRGAGTKTAAGGAAGLLEVATYPDADIVLCASSGTAALEAVLAAIEAGKTIALA
                     NKEVLVMAGGLMTEAARRRGVAILPVDSEHNAIHQCLAGRRREEVRRLVLTASGGPFR
                     GRSGVGLDRVGPEDALQHPTWRMGRKITIDSATLMNKGLEVIEARWLFDMPADAIDVV
                     IHPQSVVHSMVELVDGSILAQLGVTDMRLPIQYAFSYPERWASPLPGLDLAACGRLDF
                     QLPDHQSFPCLGLAYRALREQGGLPVVLNAANEVAVEAFLGGRLKFTAIPEVISRAME
                     AHDGGPADSLEAVRAIDRRARARAREVAAGLDLKV"
     gene            1123..2442
                     /gene="rseP"
                     /locus_tag="PKK95_15980"
     CDS             1123..2442
                     /gene="rseP"
                     /locus_tag="PKK95_15980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOC19769.1"
                     /translation="MLTTILAFVFVLGVLIFVHELGHFIAARRIGIRVITFSLGFGPK
                     VFSFRRGDTEYAISAVPLGGYVKMAGESPDDPRSGKDDEFLSKSKWQRFQVLIMGPAM
                     NVLLAIVLMWVVLLQGAQVPAFIDEPPVIGSVAEGSPAERAGIERGDRIVRVSGREVA
                     TWEDLSIAIGTKANREVPVELVRDDRTITVRVTPDPQTKYEVGDIGVFPDVHPSVLAV
                     VKGDPADRAGMQPGDVVLAVNGQPMSVSQQLSNAIAAHPGEQITVTVRRDGETRDVLV
                     TPEKRGAKGIIGISIGEELKTIDPGPLEALQMSLQRNYEFGGLIFRTLGGLLTRETSP
                     KQLMGPVAIAQLSGEYAAAGFIALLSLMASISLNLGILNLLPIPVLDGGHIFIMAIEG
                     LARRDLSMKVKEQLLLAGFFLLMILMVTVIYNDLTRIQWVERLMFWR"
     gene            2616..>3037
                     /locus_tag="PKK95_15985"
     CDS             2616..>3037
                     /locus_tag="PKK95_15985"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOC19770.1"
                     /translation="MALAVLAVLACAPARAQGQAISPEQRGRIDAIFSRFTLSTPGCA
                     VGVARGGETVFEKAYGLADLEREVPNRPDTIFEAGSIAKQFTAAAVLLLAKDRKLSLD
                     DPIRRYVPELPEHNAPVTLRHALTHTGGLRDWGEIAALG"
BASE COUNT          448 a         1007 c         1102 g          480 t
ORIGIN      
        1 gacgccatcg agacgagcgc cgggctgaag cgctcggcct gctcgcgggg agcaacgggg
       61 cgctgctgtg cgagcaggcg gagcgcttca gggcggcgct cgtctcgctg gcgtcggcga
      121 gcgcgctggc tgccgcctct gccgcgcttc ggggcgcggg cacgaagacc gccgcgggcg
      181 gagccgccgg gctgctggag gtcgccacct accccgacgc cgacatcgtc ctgtgcgcct
      241 cgtcgggaac cgcggccctc gaagcggtgc tggccgccat cgaggccggc aagaccatcg
      301 ccctggcgaa caaggaagtg ctcgtcatgg cgggcgggct gatgacggag gcggcgcggc
      361 ggcgaggcgt ggccatcctc ccggtggaca gcgagcacaa cgccatccac cagtgcctgg
      421 ccgggcgaag gcgcgaggag gtacgccgcc tggtgctcac cgcctcgggg ggaccgtttc
      481 gcggccggtc gggcgtgggg ctcgaccggg tgggccccga ggacgccctc cagcatccaa
      541 cctggcggat ggggcggaag atcaccatcg attcggcgac gctgatgaac aaggggctgg
      601 aggtgatcga ggcccgctgg ctcttcgaca tgccggccga cgcgatcgac gtggtgattc
      661 acccgcagtc ggtcgtgcac tccatggtcg agctggtgga cggctcgatc cttgctcagc
      721 ttggcgtgac cgacatgcgg ctgccgattc agtacgcgtt ttcctacccc gagcgttggg
      781 cctcgccgct gccggggctc gacctggcag catgcgggag gctcgacttc cagctgccgg
      841 accaccagag ctttccctgc ctcgggctgg cctaccgtgc cctccgcgag cagggcgggc
      901 tgccggtggt gctgaacgcg gccaacgagg tggccgtgga agcgttcctc ggcggccggc
      961 tgaagttcac ggcaatcccg gaggtcatct cgcgcgcgat ggaggcgcac gatggcgggc
     1021 ccgcggattc actcgaggcg gttcgcgcca tcgaccggcg agcccgtgcg cgcgcccgcg
     1081 aggtggcggc gggcctggat ttgaaggtgt gagggtgtcc ccgtgctgac caccatcctg
     1141 gcgttcgtct tcgtcctggg cgtgctgatc ttcgttcacg agctgggcca cttcatcgcg
     1201 gccaggcgga tcggcatccg ggtgatcacc ttttcgctgg gcttcggccc gaaggtgttc
     1261 tcgttccggc gcggcgacac cgagtacgcg atcagcgcgg tcccgctcgg gggctacgtc
     1321 aagatggccg gcgagtcgcc cgacgacccg cgcagcggga aggacgacga gttcctctcg
     1381 aagagcaagt ggcagcggtt ccaggtcctg atcatggggc cggcgatgaa cgtgctgctg
     1441 gccatcgtcc tcatgtgggt cgtgctcctc cagggcgccc aggtgccggc gttcatcgac
     1501 gagccgccgg tgatcggctc ggtggccgaa gggtcgcccg cagagcgcgc ggggatcgag
     1561 cggggtgacc ggatcgtgcg ggtgtcgggg cgcgaggtgg ccacctggga ggatctgtcg
     1621 atagcgattg ggacgaaggc caaccgcgaa gtccccgtcg agctggtgcg cgacgatcgg
     1681 acgatcaccg tgagagtgac gcccgatccg cagacgaagt acgaggtcgg ggacatcggg
     1741 gtgttcccgg acgtgcaccc gagcgttctc gccgtggtca agggcgatcc ggccgaccgg
     1801 gccggcatgc agccgggcga cgtggtgttg gcggtgaacg gccagccgat gagcgtctcc
     1861 cagcagctgt cgaacgccat cgccgcacac cccggcgagc agatcaccgt cacggtacgg
     1921 cgcgacggcg agacgcgcga cgtgctcgtg acgccggaga agcgcggcgc caagggcatc
     1981 atcggcatct ccatcggcga ggagctgaag acgatcgatc cgggcccact cgaggcgctg
     2041 cagatgagcc tccagcgcaa ctacgagttc ggcgggctga tcttccggac gctggggggg
     2101 ctgctcaccc gcgagacgtc gcccaagcaa ctcatggggc cggtggcgat cgcgcagctg
     2161 tcaggagagt acgccgcagc cgggttcatc gcgctgctga gcctgatggc ctcgatcagc
     2221 ctcaacctcg gcatcctgaa cctgctcccc atccccgttc tcgacggcgg gcacatcttc
     2281 atcatggcga tcgaagggct cgcgcggcgc gacctcagca tgaaggtgaa ggagcagctg
     2341 ctgctggcgg ggttcttcct gctgatgatc ctgatggtca ccgtgatcta caacgacctg
     2401 acccggatcc agtgggtcga gcgcctgatg ttctggcggt agatccgctc cggggcggta
     2461 caatcgccgg catgtcccag tggcacaggc gtctcgcctg tggtgcagtg ccacagtggc
     2521 acaagcgtct ctcctgtggc gcagtggcac agtggcacag gcgtctcgcc tgtggcgtag
     2581 tggcacagtg gcacaggcgt ctcgcctgtg gcgtagtggc acttgccgtc ctcgccgtcc
     2641 ttgcctgcgc gccggcgcgc gcgcaggggc aggccatatc cccggaacag cgaggccgga
     2701 tcgacgcgat cttctcgcgg ttcacccttt ccacgccggg atgcgcggtc ggcgtcgccc
     2761 gcggcggcga gacggtgttc gagaaggcct acgggctcgc tgacctcgag cgcgaggtgc
     2821 cgaaccggcc cgacaccatc ttcgaggcgg gttcgatcgc caaacagttc accgccgcgg
     2881 ccgtcctcct gctcgccaag gaccgcaagc tctcgctcga cgatccgatc cgccgctacg
     2941 tgccggaact gcccgagcac aacgccccgg tgacgcttcg ccacgctctc acgcacacgg
     3001 gcgggcttcg cgactggggc gagatcgcgg cgctcgg
//
LOCUS       DAOPQX010000414         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_35209_length_3033_cov_4.444594, whole genome shotgun sequence.
ACCESSION   DAOPQX010000414 DAOPQX010000000
VERSION     DAOPQX010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35209_length_3033_cov_4.444594"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PKK95_15990"
     CDS             <1..1298
                     /locus_tag="PKK95_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19771.1"
                     /translation="LWGVLVIDMIQFFIKMTAVIAAAYFAVTLPEVGGLSGLVSKLSN
                     TAGPNGINYLSILPDFSSNWDIAVAIFVMPIAVQWWAVWYPGAEPGGGSYIAQRMLAS
                     KSEKDSLGAVLFFNVAHYVLRPWPWILTGLASIIVYPELSDIQKAFPHLDPALIGHDI
                     AFPAMLKFLPVGFIGLMVGGLIAANSSTILTHLNWGASYLVHDFYRRFIRRDASEKHY
                     VMAGRLVTVMLFLLSSSLVFVLDTAKDAFDLILQIGAGTGLLYLVRWFWWRVTAWCEV
                     VAMVSSFLVSAGLFVLAKNGVTFSTHLALLTTIAITTVCWVATAYLGPQTDRQTLIAF
                     YRKVRPFGPGWEAVRREAGLPPSAAGAGGENIPLALLGWVTGCAVIWSSLFTVGNVLY
                     GRWGYAAALFAVFLASGLVLLAVIRRLWTPDPVAQEYQE"
     gene            1381..2748
                     /locus_tag="PKK95_15995"
     CDS             1381..2748
                     /locus_tag="PKK95_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19772.1"
                     /translation="MATLSLLLSVALFAPPVSALPAVPEPLPVVQAPTAAGQVEPPPA
                     PERTVDEVVSEVENLRGWTFKRPVKRERTSIEEAKRDLRLNLERTLPAERRALTRAFL
                     ATAGFIPPGLDIEATLLSLLEQQVAGYYQPETATLHLVQRPQRMPAFVERIVLVHELT
                     HALDDQHADIRALTEPDAARTEDMDIVLTSLVEGSATSLMLQHMMRAQAAGQVNPGEL
                     MQYAAEEMERAKVFEALPRYFSALFGSYLVGAGFLAQGELASILTQPDNRVIGERFLA
                     ARRALPLSSEQMLHPEKYWDPAQRDDPTAVDDAAVERWLAAPNRWVVHRDTLGELLTA
                     VLTRSRDSKVAVDQLQTLAAWTNAAATGWDGDRFYLLASGADAIEAQRSLDNPRGVWI
                     TTWDSERDRDEFRAALEAGSVPAGTAAVPFGTTHAAVFFGLSDAEREGLIRELPRVGG
                     QFFKR"
     gene            complement(2738..>3033)
                     /locus_tag="PKK95_16000"
     CDS             complement(2738..>3033)
                     /locus_tag="PKK95_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19773.1"
                     /translation="GLPLVAGCGLDLYQVHWYDRLERRSPLSRPPARELDRPLLLGEF
                     PTSRSRLTAAEVIAAAREAGYSGALAWSARAHDEHSNLAALEEALGVTSQTNA"
BASE COUNT          420 a         1065 c         1056 g          492 t
ORIGIN      
        1 ggctgtgggg cgtgctcgtc atcgacatga ttcagttctt catcaagatg acggcggtga
       61 tcgcggcggc gtacttcgcg gtgacgctgc ccgaggtcgg ggggctgtcg ggcctggtct
      121 cgaagctgtc gaacacggcc ggcccgaacg gcatcaacta cctgagcatc ctgccggact
      181 tcagcagcaa ctgggacatc gccgtcgcga tcttcgtgat gccgatcgcg gtccagtggt
      241 gggccgtgtg gtaccccggg gccgagccgg gcggcggcag ctacatcgcc cagcggatgc
      301 tggcgtcgaa gtcggagaag gactcgctcg gcgccgtgct gttcttcaac gtcgcgcact
      361 acgtgctgcg gccctggccg tggatcctga ccgggctcgc gtcgatcatc gtgtaccccg
      421 agctgtcgga catccagaag gccttccccc acctcgaccc cgcgctgatc ggccacgaca
      481 tcgccttccc ggccatgctc aagttcctgc cggtcggctt catcgggctg atggtgggcg
      541 gcctcatcgc ggcgaactcg tccacgatcc tgacgcacct caattggggc gcgtcgtacc
      601 tggttcacga cttctaccgc cgcttcatcc gccgcgacgc ctccgagaag cactacgtca
      661 tggcggggcg cctcgtgacg gtgatgctct tcctgctgtc ctcctcgctc gtgttcgtcc
      721 tcgacaccgc gaaggacgcg ttcgacctga tcctgcagat cggcgccggc accggcctgc
      781 tctacctggt gcgctggttc tggtggcgcg tgaccgcgtg gtgcgaggtc gtcgcgatgg
      841 tcagttcctt cctggtgtcg gccgggttgt tcgtgctcgc caagaacggc gtcaccttca
      901 gcacccacct ggccctgctc accacgatcg ccatcaccac ggtgtgctgg gtggcaaccg
      961 cgtacctcgg gcctcagacc gatcgccaga ccctcatcgc gttctaccgg aaggtcaggc
     1021 ccttcggacc ggggtgggag gccgtccggc gcgaggccgg gcttccgccc tcggccgccg
     1081 gcgcaggggg cgagaacatc ccgctggcgc tgctcggctg ggtcaccggc tgcgcggtca
     1141 tctggtcgtc gctcttcacg gtcgggaacg tgctctatgg gcgatggggc tacgcggccg
     1201 ccctgttcgc cgtgttcctt gccagcggcc tcgtgctgct ggccgtgatc cggcgcctgt
     1261 ggacccccga cccggtggcc caggagtacc aggagtagaa aggggccccg cggtgccgag
     1321 cccgcccggc accgtggggc ttgacgtggc ccgagcgcgc cggtgccgta gaatactcga
     1381 gtggctaccc tctcactgct gctctcggtg gcgctgttcg cgccacccgt ctccgcattg
     1441 cctgccgtcc ccgagccgct gccggtcgtc caggccccga cggccgccgg acaggtcgag
     1501 ccgcctccgg ccccggaacg aaccgtcgac gaggtggtgt cggaggtcga gaacctccgc
     1561 ggctggacct tcaagcgtcc ggtcaagcgc gagcggacgt cgatcgagga ggccaagcgc
     1621 gacctgcggc tgaacctgga gcggacgctg cccgccgagc gccgcgccct cacgcgcgcg
     1681 ttcctggcga cggccggctt catcccgccg ggcctcgaca tcgaggccac gctcctgtcg
     1741 ctcctggaac agcaggtggc cgggtactac caacccgaga cggcgacgct gcacctggta
     1801 cagcgcccgc agcggatgcc ggcattcgtc gagcgcatcg tgctcgtgca cgagctgacg
     1861 cacgccctcg acgatcagca cgccgacatc cgggcgctga ccgagccgga cgccgcacgc
     1921 accgaggaca tggacatcgt cctcacgtcg ctcgtcgagg gctcggcgac ctcgttgatg
     1981 ctgcagcaca tgatgcgggc gcaggcggcc gggcaggtca acccgggcga gctgatgcag
     2041 tacgcggccg aggagatgga gcgggccaag gtgttcgagg cgctgccgcg ctacttctcg
     2101 gccctgttcg gatcgtacct cgtcggtgcc ggcttcctcg cgcagggaga gctggcatcc
     2161 atcctgaccc agcccgacaa ccgggtcatc ggcgagcgtt tcctcgctgc gcgccgggcg
     2221 ctcccgttgt cgagcgagca gatgctccac ccggagaagt actgggaccc ggcccagcgc
     2281 gacgatccga ccgcggtcga tgacgcggcc gtcgagcggt ggctggcggc gcccaatcgc
     2341 tgggtcgtgc accgggacac gctcggcgag ctgctgaccg cggtgctgac tcgaagccgc
     2401 gactcgaagg tggccgtaga tcaactccag acgcttgccg cctggaccaa cgcggcggcg
     2461 acgggatggg acggcgatcg gttctacttg ctcgcgtccg gagcggacgc gattgaggcg
     2521 cagcgctcgc tcgacaaccc tcgcggcgtc tggattacca cctgggacag cgagcgcgac
     2581 cgcgacgagt tccgggccgc cctggaggcg gggtcggtcc ccgcgggcac ggctgccgtt
     2641 ccgttcggca cgactcacgc ggcggtcttc ttcggtctca gcgacgcgga gcgcgaaggg
     2701 ctcatccgcg agctgccgcg cgtcggcggg cagttcttca agcgttagtc tgagacgtca
     2761 ccccgagcgc ttcctcgagc gccgcgaggt tggagtgctc gtcgtgggcg cgggccgacc
     2821 aggccagcgc accgctgtag ccggcctcgc gcgcggcggc aatcacctcg gcggcggtga
     2881 gccgcgaccg ggacgtgggg aactcgccga gcagcagcgg ccggtccagc tcccgggccg
     2941 ggggccgtga cagcggagag cggcgctcca gccggtcgta ccagtggacc tggtagagat
     3001 cgaggccgca cccggcaacg agcgggaggc cgc
//
LOCUS       DAOPQX010000415         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_35268_length_3029_cov_5.071957, whole genome shotgun sequence.
ACCESSION   DAOPQX010000415 DAOPQX010000000
VERSION     DAOPQX010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35268_length_3029_cov_5.071957"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..2894)
                     /gene="mfd"
                     /locus_tag="PKK95_16005"
     CDS             complement(273..2894)
                     /gene="mfd"
                     /locus_tag="PKK95_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOC19774.1"
                     /translation="MPSPPFHGRLADWASEIRAARAAGETVLFVAATPGRAERVVELL
                     KDYELLAVPVFVDPRHRDNLQARGEMVHGASVLVATGLLSRGFRLPAAALRIFAETDV
                     FEEERRPSERRRAAARTFLSDFRDLKVGDHVVHVDHGIGVFVGLKQIAVDPYGGPTQE
                     FLELRYAGEDKLFVPVERLDLLQKYTGAGRPALDRLGGTTWEKAKTRVKKAMRDMAEE
                     LLKLYASRKALAGHAFSPDTHWHEEFDAAFPYELTPDQQAAVDDIRRDMESPTPMDRL
                     LCGDVGYGKTEVAMRAAFKAVMDGKQVAFLAPTTVLAFQHLKTLRERFAGFPVTIDMV
                     SRFRTRAEIKKTLDDLAAGKVDIIVGTHRLLSKDVAFKDLGLLVVDEEQRFGVAHKER
                     IKQMRRRVDVLTMTATPIPRTLNMSLVGIRDMSVIETPPKDRLAIQTNVVRFDQDLIA
                     RAVRGELSRGGQVYFIHNRVESIYSIGQLLQRLIPEARVVVGHGQMSEDALERAMVDF
                     VARKYDVLLATTIVENGLDIPNVNTIVINRADRYGLSQLYQLRGRVGRSDRPAYAYLL
                     VPPGNTLSPVARKRLAAIREFSELGSGFRVAALDLEIRGAGNLLGGEQSGHIEAIGFE
                     MYTKLLEQTVRELKGEEIEDESRATVNLGVDLRIDEEYVPEQNQRLGLYRRVASARSD
                     AEIDSIVAEVEDRYGPMPPEVLNLVDYGRIRVLADRLGVEKIDRQGAVVVLTFKGQNG
                     PEPERVLRLLREQPDVTLAPPSGIKLDLNGMTRLVRGNSPRRGLEEASGGLPRRSLEE
                     ASGGLPRRSLEEAKGGQASTARGAARLPRRGEAAKARSWWTARATEEEVKPASARRPS
                     SGRRRRIPAPRAASSPG"
BASE COUNT          417 a         1035 c         1098 g          479 t
ORIGIN      
        1 ccaagctgcg ccaccggcgc gggcgtcgcg ggctgttgcg gcgcaggctg cggcggcgcg
       61 gccggcggct gctggggcgg agcctgctgc gccattcccg tggcgccgag cgaggccgcg
      121 aacaacaggg cgagcagggg acgaaacagg ttcgacatag agtgagcttt ccgcggcccg
      181 ccgtagccgc cggcacgttt tcaagccggc gcggaggcga gagccgaatc ccttatctta
      241 ctgtccgccc gccagttccg ccagcagcgc ggtcaccctg gcgaggacgc cgcccggggc
      301 gcggggatcc tccttcgccg gccggaggat ggcctccttg cggaagccgg cttcacctct
      361 tcctcggtgg cgcgggcggt ccaccagctg cgcgccttcg cggcctcgcc gcggcggggc
      421 aggcgcgccg caccccttgc cgtgctcgct tggcccccct tcgcctcttc gaggctacgg
      481 cggggcaagc ccccagacgc ctcttcgagg ctacggcgag gcaggccccc agacgcctct
      541 tcgaggccac ggcgcggcga gttccctcgg acgagccggg tcatcccgtt caggtcgagc
      601 ttgatccccg agggcggcgc cagcgtcacg tcgggctgct cgcgcagcag ccgcaacacc
      661 cgctcgggct cggggccgtt ctggcccttg aaggttagga cgaccacggc cccctggcgg
      721 tcgatcttct ccacgcccag ccggtcggcc agcacgcgga tgcgcccgta gtccaccagg
      781 ttgagcacct cgggcggcat cggcccgtag cggtcttcga cctcggcgac gatcgagtcg
      841 atctcggcgt ccgagcgcgc ggacgcgacg cggcggtaga ggccgagccg ctggttctgc
      901 tcggggacgt actcttcgtc tatcctcagg tccacgccaa ggttcacggt cgcgcgcgac
      961 tcgtcctcga tctcctcgcc cttcagctcg cgcacggtct gctcgagcag cttcgtgtac
     1021 atctcgaagc caatcgcctc gatgtggccg ctctgctcgc cgccgagcag gttccccgcg
     1081 ccgcggatct cgaggtcgag cgccgccacg cggaagccgc tgcccagctc gctgaactcg
     1141 cggatggccg ccaggcgctt tcgcgccacc ggcgacagcg tgttgccggg cggcaccagc
     1201 aggtaggcgt aggcgggacg gtcggatcgc ccgacgcgcc cgcgcagctg gtagagctgc
     1261 gagagtccgt accggtcggc gcggttgatg acgatggtgt tgacgttcgg gatgtcgagt
     1321 ccgttctcga cgatcgtggt ggcgagcagc acgtcgtact tccgcgcgac gaaatccacc
     1381 atcgcgcgct cgagcgcgtc ctcgctcatc tgcccgtgcc ccaccacgac gcgcgcctcg
     1441 gggatgagcc gctgcagcag ctggccgatc gagtagatcg actccacccg gttgtggatg
     1501 aagtacacct ggccgccgcg cgacagctcg ccgcggacgg cgcgggcgat caggtcctgg
     1561 tcgaagcgca cgacattggt ctggatcgcc aggcggtcct tcggcggcgt ctcgatgacc
     1621 gacatgtcgc ggatccccac gagcgacatg ttcaacgtcc tcgggatcgg cgtcgccgtc
     1681 atcgtcagca cgtccaccct gcggcgcatc tgcttgatgc gctccttgtg ggcgacgccg
     1741 aagcgctgct cctcgtccac caccagcagg ccgaggtcct tgaaggccac gtccttcgaa
     1801 agcaggcggt gggtgccgac gatgatgtcc accttgccgg cggccaggtc gtcgagcgtc
     1861 ttcttgatct cggcgcgcgt gcggaagcgg ctcaccatgt cgatggtcac cgggaagccg
     1921 gcgaaccgct cgcgcagcgt cttgaggtgc tggaacgcga gcacggtggt gggcgcgagg
     1981 aaggccacct gcttgccgtc catcaccgcc ttgaaggcgg cgcgcatcgc cacctcggtc
     2041 ttgccgtagc cgacgtcgcc gcagagcagg cggtccatcg gcgtcggcga ctccatgtcg
     2101 cgccggatgt cgtcaactgc cgcctgctgg tcgggcgtca gctcgtaggg aaacgcggcg
     2161 tcgaactcct cgtgccagtg ggtgtcgggc gagaaggcat ggccggcgag cgccttgcgc
     2221 gaggcgtaga gcttcagcag ctcctcggcc atgtcgcgca tcgccttctt gacgcgcgtc
     2281 ttggccttct cccacgtggt gccgcccagg cggtcgagcg cgggacgccc ggcccccgtg
     2341 tacttctgca acaggtcgag ccgctcgacg gggacgaaga gcttgtcctc gcccgcgtag
     2401 cgcagctcga ggaactcctg cgtcgggccg ccgtacggat cgaccgcgat ctgcttgagg
     2461 ccgacgaaga cgccgatgcc gtggtccacg tggacgacgt ggtcgcccac cttgaggtcg
     2521 cggaagtcgg agaggaaggt gcgggcggcg gcgcggcggc gctcggacgg ccggcgctcc
     2581 tcctcgaaga cgtcggtctc ggcaaagatg cgcagcgcgg cagcgggaag gcggaagccg
     2641 cgcgaaagga ggccggtggc gaccagcacg gaggcgccgt gcaccatctc gccgcgcgcc
     2701 tgcaggttgt cgcgatggcg cggatcgacg aagacgggga ccgccagcag ctcgtagtcc
     2761 ttcagcagct cgacgacacg ctcggcgcgg cccggcgtgg cggcgacgaa gagcacggtc
     2821 tcgccggcgg cgcgcgcggc gcgaatctcg gaggcccagt cggcaaggcg gccgtgaaaa
     2881 gggggagacg ggatacttgt cgtgctggtg gtgctgatgg tgccggtggt gctggaggtg
     2941 ctagtgccgg aggtgccggt ggtgctggtg ctggaggtgc tgggggtgct ggtgccggag
     3001 gtgccggtgg tgctggaggt gctagtgcc
//
LOCUS       DAOPQX010000416         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_35466_length_3016_cov_5.796015, whole genome shotgun sequence.
ACCESSION   DAOPQX010000416 DAOPQX010000000
VERSION     DAOPQX010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35466_length_3016_cov_5.796015"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PKK95_16010"
     CDS             <1..244
                     /locus_tag="PKK95_16010"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOC19775.1"
                     /translation="LEIFANDVKCTHGATVGQISADALFYLRARGIGLAEARRILVRA
                     FAGEVIGRMKLEPVRELLDRLIAERLPDRPGEESLE"
     gene            241..1515
                     /locus_tag="PKK95_16015"
     CDS             241..1515
                     /locus_tag="PKK95_16015"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOC19776.1"
                     /translation="MNQGVGLGASSTGAGLRARPFDPSSVGSDFPILSQLVHGKPLSY
                     LDNAATTQKPVPVVEAIGDFYLRDNANIHRGVHLLSERATAAYEGAREAVQRFINARE
                     AREVVFVRGTTEAVNLVAQSLVRSGLKEGDEIVVSVLEHHSNFVPWKMAAEATGARLR
                     VVPITDAGDLRLDEYERMLSPRTRLVALGHVSNALGTINPVAEIVRLAHERGVPVLLD
                     GAQAVAHLAVDVQAIDCDFYAFSGHKLYGPTGIGVLYGKADRLDALPPWQGGGDMIRS
                     VTLERVDYNTLPAKFEAGTPDIAGAIGLAAAIRYLESFDRAAAFAHEDELLGYAVERL
                     ERIPGVRLVGRGMPRTSIVSFVMDQVHPHDIGTILDGEGVAIRAGHHCCQPLMHRLGL
                     DATARASFAFYNRKEDVDRLADGLDRVREVLG"
     gene            1515..1961
                     /locus_tag="PKK95_16020"
     CDS             1515..1961
                     /locus_tag="PKK95_16020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOC19777.1"
                     /translation="MPDLRELYQEVILDHHKRPRNFGPLPEGNHHAKGHNPLCGDRIT
                     VHVQVADGIIRDVRFEGAGCAISRASASLMTDAVKGRPIAEAERLFEEFHRLVTTGVD
                     EEVEASLGKLNVFCGVREFPSRVKCASLAWHTLHSALGDEGEASTE"
     gene            2004..2342
                     /locus_tag="PKK95_16025"
     CDS             2004..2342
                     /locus_tag="PKK95_16025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOC19778.1"
                     /translation="MADKMKGDPVAALVLKPAIIEALSQVYDPEIPVNIWELGLIYDI
                     RIDSESRVEIEMTLTAPGCPAAQALPYEVDQKVREVPGVKDVYVEVVWEPAWTTDRMS
                     EAARLQLGMF"
     gene            complement(2348..>3016)
                     /locus_tag="PKK95_16030"
     CDS             complement(2348..>3016)
                     /locus_tag="PKK95_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC19779.1"
                     /translation="REFTDPALAGQVSGSPYRFNTYAGASHLFFLTQGYAVLDNPTMP
                     IVGPGETANDTYVEQLVASAQAAVDKVVEIGVGDRDRIGVGGHSYGAFMTANLLAHSR
                     LFKAGIARSGAYNRSLTPFGFQSERRTFWEVPEVYAAMSPFWFAHKVKDPILLIHGEA
                     DNNSGTFPIQSERFYMALKGHGATVRYVTLPNESHGYAARESVLHTLAEMIGWFDRYV
                     KGED"
BASE COUNT          468 a         1011 c         1075 g          462 t
ORIGIN      
        1 gctcgagatc ttcgccaacg acgtgaagtg cacgcacggc gccacggtgg ggcagatcag
       61 cgcggacgcg ctgttctacc tgcgcgcgcg cggcatcggc ctggccgagg cgcggcgcat
      121 cctggtgcgg gcattcgccg gagaggtcat cggccggatg aagctcgagc cggtgcgcga
      181 gctgctcgac cgcctgatcg ccgagcggct accggaccgc ccgggggaag agagcctgga
      241 atgaaccaag gggtgggcct tggcgcttcg tcgacggggg cgggcctgcg tgcccgcccg
      301 ttcgatccct cctcggtcgg ttccgacttt ccgatcctct cgcagctcgt gcacggcaag
      361 ccgctcagct acctcgacaa tgccgcgacg acgcagaagc cggtcccggt ggtggaggca
      421 atcggcgact tctacctccg cgacaacgcc aacatccatc gcggcgtcca cctgctgagc
      481 gagcgtgcga cggccgccta cgagggcgcg cgcgaggcgg tgcagcggtt catcaacgcg
      541 cgcgaggccc gcgaggtggt cttcgtgcgc gggacgaccg aggccgtcaa cctggtcgcc
      601 cagtcactgg tgcggtcggg cctgaaggaa ggcgacgaga tcgtcgtgtc ggtgctcgag
      661 caccactcga acttcgtccc gtggaagatg gcggccgagg cgacgggcgc acgcctccgg
      721 gtggtgccaa tcaccgacgc gggcgacctg cggctcgacg agtacgagcg gatgctgtcg
      781 ccccggacgc gcctggtcgc cctcggccac gtgtccaacg cgctcgggac catcaacccc
      841 gtcgccgaga tcgtgcgcct cgcgcacgaa cgcggcgtcc cggtgctcct ggacggcgcg
      901 caggcggtcg ctcatcttgc cgtggacgtt caggcgatcg actgcgactt ctacgcgttc
      961 tcggggcaca agctctacgg cccgaccggc atcggcgtgc tttacggcaa ggccgaccgc
     1021 ctcgacgccc tgcccccgtg gcaggggggc ggcgacatga tcaggtcggt gaccctcgag
     1081 cgggtggact acaacaccct gccggcgaag ttcgaagccg gaacgccgga catcgcgggc
     1141 gcgatcggcc tcgcggccgc catccggtac ctcgagagct tcgaccgcgc ggccgccttc
     1201 gcccacgagg acgagctgct cggctacgcg gtcgagcgcc tcgagcgcat tccgggcgtc
     1261 cgcctggtgg ggcgcgggat gccgcggacg agcatcgtct cgttcgtgat ggaccaggtc
     1321 cacccgcacg acatcgggac gatcctcgac ggcgaagggg tggcgatccg tgccggccat
     1381 cactgctgtc agccgctcat gcaccgtctc ggcctcgacg cgaccgcgcg cgcctccttc
     1441 gcgttctaca atcggaaaga ggacgtcgac cggcttgcgg acgggctcga tcgcgtgcgg
     1501 gaggtgttgg gctgatgccg gatctgcgcg agctgtacca ggaggtcatc ctcgaccatc
     1561 acaagcggcc gaggaacttc ggcccgctgc ccgaggggaa ccaccacgcc aagggccaca
     1621 atccgctgtg cggcgaccgc atcacggtgc acgtgcaggt ggccgacggt atcatccgcg
     1681 acgtgcgctt cgagggggcc gggtgcgcca tctcgcgcgc ctcggcgtcg ctgatgaccg
     1741 acgcggtgaa gggcaggccc attgccgagg ccgagcgcct cttcgaggag ttccaccggc
     1801 tggtgacgac cggcgtggac gaggaggtcg aggcgagcct cggcaagctg aacgtcttct
     1861 gcggcgttcg cgagttcccg tcccgcgtga agtgcgccag cctggcctgg cacacgcttc
     1921 actcggcact cggggacgag ggcgaagcgt cgaccgagta gggcggattc gggattcggc
     1981 gatcagtgga gttggagcga gggatggcgg acaagatgaa gggcgacccg gtggcagcgc
     2041 tcgtgctgaa gccggcgatc atcgaggcgc tgagccaggt ctacgacccg gagatcccgg
     2101 tcaacatctg ggagctgggg ctgatctacg acatccggat cgactcggag tcgcgcgtcg
     2161 agatcgagat gacgctcacc gccccgggat gcccggccgc tcaggcgctt ccctacgagg
     2221 tcgaccagaa ggtgcgcgag gtgcccggcg tgaaggacgt ctacgtggag gtcgtctggg
     2281 agcccgcctg gacgaccgac cggatgtcgg aagccgcccg ccttcagctg ggaatgttct
     2341 agcgctccta gtcttccccc ttcacgtacc ggtcgaacca cccgatcatc tcggccagcg
     2401 tgtgcagcac cgactcgcgg gccgcgtagc cgtgcgactc gttgggcagc gtcacgtagc
     2461 ggacggtggc gccatggccc ttcagcgcca tgtagaagcg ctcggactgg atcgggaacg
     2521 tgcccgagtt gttgtcggcc tccccgtgga tgagcaggat cggatccttc accttgtggg
     2581 cgaaccagaa cggggacatc gccgcgtaga cctcgggcac ctcccagaag gtccgccgct
     2641 cgctctggaa gccgaacggc gtcagcgagc ggttgtaggc gccgctgcgc gcgatcccgg
     2701 ccttgaacag gcgggaatgg gcgagcaggt tggcggtcat gaaggcgccg tagctgtgcc
     2761 cgccgacgcc gatccggtcg cggtcgccga ccccgatctc gaccaccttg tcgaccgctg
     2821 cctgcgcgct cgccacgagc tgctccacgt aggtgtcgtt ggccgtctcg ccgggcccga
     2881 cgatgggcat cgtcgggttg tcgaggacgg cgtagccctg cgtcaggaag aacaggtgcg
     2941 acgcccccgc gtaggtgttg aaccggtagg gtgagccgga cacctgcccg gccagcgcgg
     3001 gatcggtgaa ctcccg
//
LOCUS       DAOPQX010000417         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_36344_length_2961_cov_4.496215, whole genome shotgun sequence.
ACCESSION   DAOPQX010000417 DAOPQX010000000
VERSION     DAOPQX010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36344_length_2961_cov_4.496215"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="PKK95_16035"
     CDS             complement(<1..1686)
                     /locus_tag="PKK95_16035"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC19780.1"
                     /translation="MTTSLRLLQAEALVHTGRHARAAVVVAEAAGQAEPAPMETLAEV
                     NRVAGVALAASGNLTHAGAAFERAARILATVGNRTGRDDLLEQYARAVWPAVRREPIP
                     FPDRPRRDHLPPPPLRVAVRLDALTRLVPAPGAAADSAAVRAATILDAGGYPEVLAHE
                     LAGLLIEAGSTVAGVLACRTGEGPAEVHAWWGCSWREALALAAAPPRRIDLGNWHGRR
                     FEIAVRPAEGMETALALVAAEKLARSARWQERARREERERSALWPVEPGGDSGDAIFL
                     SQGMIDLVASVRRVAPLNVLVLLTGETGTGKEVVARVIHAASGRAAGPFVPFNCTAVP
                     RELIDSQLFGYRRGAFTGAAADFPGVIRAAAGGTLFLDEIGELGLDVQPKLLRFLESG
                     EIQPLGDARPTRVEVRVVASTNANLEGLVADGRFREDLYYRLAVVRFPIPPLRERREE
                     VPALAQHFLERAARDFQKEPLSLADESVEYLTLYRWPGNVRQLANEMRRLAALAEKGA
                     VLMPEHLDPAITAGRRTVPAAERALADEELVVRLDQPLDAAVEHVERAAIQRAM"
BASE COUNT          441 a         1002 c         1072 g          446 t
ORIGIN      
        1 catcgcgcgc tggatggcgg cgcgctccac gtgttcgacg gcggcgtcga gcggctgatc
       61 gaggcggacg accaattcct cgtccgccaa ggcgcgttcg gccgcgggga ccgtccggcg
      121 tcccgccgtg atcgccgggt ccaggtgttc gggcatgagc acggcgccct tctcggcgag
      181 ggcggcgagg cggcgcatct cgttggcgag ctgccgcacg ttgccgggcc agcggtagag
      241 cgtcaggtat tcgaccgact cgtcggcgag gctgagcggt tccttctgga aatcgcgcgc
      301 cgcccgctcg aggaagtgct gcgcgagggc ggggacctcc tcgcgccgct cgcggagcgg
      361 cgggatgggg aagcgcacga ccgcgagccg gtaatagagg tcttcccgga agcggccgtc
      421 ggcgacgagg ccctcgaggt tcgcattggt cgaggcgacc acgcggacct ccacgcgggt
      481 cgggcgcgcg tcgccgagcg gctggatctc gcccgactcg aggaagcgca gcagcttggg
      541 ctggacgtcg agtcccagct cgccgatctc gtcgaggaag agggtgccgc ccgcggccgc
      601 ccggatgacg ccggggaagt cggcggccgc gcccgtgaac gcgccgcgcc ggtacccgaa
      661 cagctggctg tcgatcagct cgcgggggac cgccgtgcag ttgaacggga cgaaggggcc
      721 tgccgcccgg ccggaggcgg cgtggatgac gcgggcgacg acctccttgc ccgtgcccgt
      781 ctcgccggtg agcaggacga gcacgttgag gggggcaacg cggcggacgc tggccaccag
      841 gtcgatcatg ccctggctca ggaagatcgc gtcacccgag tcgccgcccg gctcgacggg
      901 ccacagggcg gagcgctcgc gctcttcgcg gcgggcccgt tcctgccagc gtgcgctgcg
      961 cgccagcttc tcggcggcga ccagggcgag ggcggtctcc atgccctcgg cagggcggac
     1021 cgcgatctcg aatcgccggc cgtgccagtt gccgaggtcg atccgccggg gcggcgccgc
     1081 ggcgagcgcg agcgcctcgc gccacgagca gccccaccag gcgtggacct cggccgggcc
     1141 ctcgccggtg cggcacgcca ggacgccggc caccgtggag cccgcctcga tcagcaggcc
     1201 cgcgagctcg tgcgcgagga cctcggggta gccgcctgcg tcgaggatcg tggcggcgcg
     1261 gacggccgcg ctgtcggccg cggccccagg ggccgggacg aggcgcgtga gggcgtcgag
     1321 gcgaacagcg acgcggagcg gcggcggggg caggtggtcg cgccgggggc gatcggggaa
     1381 ggggatcggc tcgcgccgga cggcgggcca gacggcgcgg gcgtactgct ccaggaggtc
     1441 gtcgcggccc gtgcggttgc cgacggtggc gaggatgcgg gcggcgcgct cgaaggcggc
     1501 gccggcatgg gtcaggttgc cgctggccgc aagggcgacc cctgcgaccc ggttgacctc
     1561 ggccagcgtc tccatgggcg ccggctcggc ctggccggcg gcctcggcga ccacgacggc
     1621 cgcccgcgca tgacggccgg tgtgaaccag cgcctccgcc tgcaagagac ggagggaggt
     1681 cgtcaagctg acgtcggtct gccgctcggc cccggcgact gctgcgcgga gtagttccgc
     1741 ggcttcactg aagcatcctc tgttgatgag aaggcgccct ctggtgacac aactccacaa
     1801 gccatagtac caagttccct tcggagcctc cgcgctccaa ccggccacgt cattcagcag
     1861 acgctcggct tcggtcggta gcccctgttc gagggccact tgcgccaagc catcgagcac
     1921 tcccctctgc gcctctccag catcaggcga aaggcgaagc gcctctcgga gaaactcctc
     1981 tgcacccgag aagttcccct cggcgagttc gatgtgtcct agatttgcaa gggcggctcg
     2041 cctgacccct cgatggccgc tctcctcccc gtgccgaagg gcaagaagcg cctcagttcg
     2101 cgccgtgggc aagtctgaga gcagatagcc tagacacgac gcgcccacgg cggcgcttcc
     2161 ccgtagccaa gcgttgggtt gctccgagag caggtttcgc cctatccgaa gatgttcagc
     2221 ggcggccgac acgagtccac gctttgcctc gatttcagcc acgaatatgt gcagagcagc
     2281 cgtagccagc gggtcgccgg ccctgacgac ggtacgtctg acgtacggga tgaacgcgag
     2341 ggcggcctcc ggcccgggtc cttcgaagag ggcaagcatc aggggaaggg ccgcccagca
     2401 agcttcgttg accttgccgt cattcgtggc gagagtgaag gcgcgctgca ggtgcgagac
     2461 cgcttgctga gtattgccag catcccgcat agcttcgccg attgcagtga ggcaccgaat
     2521 cttcgcgcag tccgaaatgc ttcctgcacg gagtaggcgt cgtgccctcg ctgaggcggc
     2581 gtgactatcg ccggtctttt gaagaagctg tgcgaggata gcctctcgct caagtccgca
     2641 aagcgactga gcttccgctc cggcgcgcag catcgacagc gcgtcacggt agagcccgtt
     2701 ggccaccagc ccggacactg tttccaaggc tgtctccgag tgagctcgtt ttagtggacc
     2761 gtcagttctg gccttggtct ggcctttcct acgggttcga ggcgttgccg cgcggtgcta
     2821 acaagcgcat caagagtctt accgtcgtcc ggacagaccg cggctccaac tctgacctgc
     2881 gacatggctc cagcgagcgc accgtcctcg cctagtggga ccgaaacggc acactttatt
     2941 cgctcgacgg taggtgtcat a
//
LOCUS       DAOPQX010000418         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_36414_length_2957_cov_4.114404, whole genome shotgun sequence.
ACCESSION   DAOPQX010000418 DAOPQX010000000
VERSION     DAOPQX010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36414_length_2957_cov_4.114404"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1517
                     /locus_tag="PKK95_16040"
     CDS             <1..1517
                     /locus_tag="PKK95_16040"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOC19781.1"
                     /translation="EFHEVVEFAILASHRDPFDPMEKAFKRLGTRFLAQTEHLHEDWT
                     LVREYPLSPDLLAMSRVWRSRDTDAYVIAAKGAPEAIADLCHLAPAETARLSRDVSAL
                     ADDGLRVLGVARAGFRPEPLPGGQHDFTFELLGLVGLEDPVRPTAVDAVRECRAAGIR
                     VVMITGDYPGTARHVARQVGLGAERLTTGAEMDGMDDAALQAAVRETNVFARVVPEQK
                     LRLVEALKANGEVVAMTGDGVNDAPALKAAHIGIAMGGRGTDVARESASLVLLDDDFT
                     SIVRAVRLGRRIFDNLQKAMAYILAVHVPIAGMSLLPVLLRWPLALFPVHIVFLELII
                     DPACSVVFEAEAAEADVMRRPPRDPGERLFGWRTLGVSLAQGATALALVAAIYAVSLA
                     GGAPEGEARALGFTTLILSNLGLIFANRSWGQTILSRIRVPNRALWMVTAGALAFLVL
                     ALSVPGLRDLFRFEPLSARGAAGAIALGVGSIAWFEVLKLANRRRRVSSEQRPT"
     gene            complement(1497..2828)
                     /gene="mgtE"
                     /locus_tag="PKK95_16045"
     CDS             complement(1497..2828)
                     /gene="mgtE"
                     /locus_tag="PKK95_16045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOC19782.1"
                     /translation="MTPRKAPLVGLTAEELRELWPGLSTEERHEAFDHMVSRTEREDF
                     FISLSARHHAELLLEFPADRRRAWLRLLAPDDVVDLIQEAPPEERETLIGQLDPFAQR
                     EVRALLAYAEDDAGGLMSPRYTRVRPDMRADEAISYLRKQARERSGAGHYVYVTDEAQ
                     RLLGVLSFRELFAAAPDALVRDVMLTDPVTAKEQDDQEELGRRFAEYDLMAIPVVDDQ
                     GRMKGIVTVDDIMDVVEEEATEDIQKFGGVQALEEPYLEVGFAGMLRKRAPWLAVLFV
                     GEMLTATAMAYFEAEIAQAVVLALFIPLIISSGGNSGSQASTLVIRAMALGEVSLRDF
                     WRVTRRELATGLALGAILGAVGLMRVAFWGAVFDSYGPHFMLLGLTVAGSLVGVVLFG
                     TLAGSLLPFILRWLNLDPASASAPFVATLVDVAGVVIYFTFAAVLLRGALL"
BASE COUNT          427 a         1045 c         1067 g          418 t
ORIGIN      
        1 aggagttcca cgaggtggtg gagttcgcga tcctggcgag ccaccgcgac ccgttcgacc
       61 cgatggagaa ggccttcaag cggctgggca cccgcttcct cgcgcagacc gagcacctgc
      121 acgaggactg gacgctggtc cgggagtacc ctctgtcgcc cgacctgctg gcgatgtccc
      181 gcgtctggcg ttcgcgcgac accgacgcgt acgtcattgc ggccaaggga gcgcccgagg
      241 cgattgccga cctgtgccac ctcgcgcccg ccgagacggc gcgcctgtcg cgcgacgtgt
      301 cggcgctcgc cgacgacggg ctgcgcgtgc tcggcgtggc gagggccggg ttccgccccg
      361 agccgcttcc cggcggccag cacgacttca ccttcgagct gctcggcctg gtcgggctcg
      421 aagacccggt gcggccgacc gcggtggacg cggtcaggga atgccgcgcc gcggggatcc
      481 gcgtcgtcat gattacgggc gactacccgg gaaccgcccg ccacgtcgcg cggcaggtgg
      541 gactcggggc ggagcggctg acgaccggcg ccgagatgga tggcatggac gacgccgcgc
      601 tgcaggccgc cgtccgcgag acgaacgtct tcgcgcgcgt ggtgcccgag cagaagctgc
      661 gcctggtcga ggcgctcaag gcgaacggcg aagtggtggc gatgaccggc gacggggtca
      721 acgatgcccc ggccctcaag gccgcccaca tcgggatcgc gatgggcggg cgcggcaccg
      781 acgtcgcccg cgagtcggcg tcgctggtgc tgctcgatga cgacttcacc tcgattgtgc
      841 gggcggtgcg gctggggcgg agaatcttcg acaacctgca gaaggcgatg gcctacatcc
      901 tggcggtgca cgtgccgatc gccggcatgt cgctgctgcc cgtcctcctt cgctggcccc
      961 tcgcgctgtt cccggtccac atcgtcttcc tcgagctgat catcgacccg gcctgctcgg
     1021 tggtcttcga ggccgaggcg gccgaggccg acgtgatgcg gcggccgccg cgcgacccgg
     1081 gcgaacggct gttcggctgg cggacgctcg gggtcagcct ggcgcaaggg gcgaccgcgc
     1141 tcgccctggt ggcggcgatc tacgcggtga gcctggcggg cggggcgccg gagggagagg
     1201 cgagggccct cggcttcacg acgctgatcc tgtcgaacct tggcctgatc ttcgcgaacc
     1261 ggtcgtgggg ccagacgatc ctgtcgcgca tccgcgtccc caaccgggcg ctgtggatgg
     1321 tgacggccgg ggcgctcgcg ttcctggtcc tcgcgctgtc ggtgccgggc ctgcgcgacc
     1381 tgttccgctt cgagccgctg tcggcccgcg gcgccgccgg ggccatcgcc ctcggcgtcg
     1441 gcagcatcgc gtggttcgag gtcctgaagc tggcgaaccg gaggagacgc gtcagctcag
     1501 agcagcgccc cacgtagcag caccgccgcg aacgtgaaat agatcacgac gccggccacg
     1561 tccaccagcg tcgcgacgaa gggggccgag gcgctggccg ggtcgaggtt cagccagcgg
     1621 aggatgaacg gcaggagcga gcctgccagg gtgccgaaca gcacgacccc gaccaggctt
     1681 cccgcgaccg tgaggcccaa cagcatgaag tgcgggccgt agctgtcgaa gaccgccccc
     1741 cagaacgcaa cgcgcatcag gccgaccgcg ccgaggatgg cgccgagcgc cagacccgtc
     1801 gccaactccc tccgggtgac gcgccagaag tcgcgcaggc tcacttcgcc gagcgccatg
     1861 gcgcggatga ccagcgtcga ggcctgcgag cccgagttgc cgccgctgct gatgatgagc
     1921 gggatgaaga gggcgagcac cactgcctgc gcgatctccg cctcgaagta ggccatcgcc
     1981 gtcgccgtca gcatctcgcc cacgaacagg acggccagcc acggcgcccg cttccgcagc
     2041 atcccggcaa agccgacctc gaggtacggc tcttcgaggg cctgcacgcc gccgaacttc
     2101 tggatgtcct cggtggcctc ctcctcgacg acatccatga tgtcgtccac cgtgacgatg
     2161 cccttcatcc gcccctggtc gtccacgacc gggatcgcca tcaggtcgta ctcggcgaac
     2221 cgccggccca gctcctcctg gtcgtcctgt tccttcgcgg tgacggggtc ggtgagcatg
     2281 acgtcgcgca ccagggcgtc gggcgccgca gcgaacagct cgcggaagga gagcaccccc
     2341 agcaggcgct gcgcctcgtc ggtgacgtac acgtagtgcc cggccccgct ccgctcgcgc
     2401 gcctgcttgc gcaggtagct gatggcctcg tcggcccgca tgtcgggccg gaccctcgtg
     2461 taacgcgggc tcatcaggcc gccggcatcg tcctcggcgt acgcgagcag cgcccgcacc
     2521 tcgcgctggg cgaaggggtc gagctggccg atcaacgtct cgcgctcctc gggcggtgcc
     2581 tcctggatga ggtccaccac gtcgtcgggg gcgagcagcc gcagccaggc ccggcgccga
     2641 tcggccggga actcgaggag cagctcggcg tggtggcgcg ccgacagact gatgaagaag
     2701 tcctcgcgct cggtgcgcga caccatgtgg tcgaaggcct cgtgacgctc ctcggtggag
     2761 aggccgggcc acagttcgcg aagctcctcc gccgtcaggc cgacgagggg tgccttgcga
     2821 ggcgtcaccg cgcacccccg cgggccggca gcgaggtggg cgcgggccgg acgaaggaca
     2881 acgcgtcagg gaagaccgag gggatcatcg gcactttcct ctgccaggtg tcgcccggct
     2941 gggaaagcgc ccgtgag
//
LOCUS       DAOPQX010000419         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_36416_length_2957_cov_3.959338, whole genome shotgun sequence.
ACCESSION   DAOPQX010000419 DAOPQX010000000
VERSION     DAOPQX010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36416_length_2957_cov_3.959338"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..949)
                     /locus_tag="PKK95_16050"
     CDS             complement(71..949)
                     /locus_tag="PKK95_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC19783.1"
                     /translation="MARNWSTSVTGSNNDYFTAANWTLASGRTFSDAEMATGSPVCVV
                     GETIRTKLFEGQNPVGSAIRVKDFSCEVIGLLKSKGQASMGRDQDDVIVMPLKTVQRR
                     LAGNQNISSMMISAKDGVDTAALKARLESLMRERRRIEEGEDDDFNILDTREIATAMS
                     GSTQILTALLGAVAAVSLLVGGIGIMNIMLVSVTERTREIGTRLAIGALEREVLLQFL
                     VEAVTLSSFGGLIGLALATTASVALTRVMGLPYVFNPQINLIAFFFSAAMGVLFGYLP
                     ARRAAGLDPIEALRHE"
     gene            complement(1275..1424)
                     /locus_tag="PKK95_16055"
     CDS             complement(1275..1424)
                     /locus_tag="PKK95_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19784.1"
                     /translation="MELLSSLNRERGLTILMATHEPQAAAYAQRVVHFLDGRVESDAV
                     QEEVS"
     gene            complement(1396..1818)
                     /locus_tag="PKK95_16060"
     CDS             complement(1396..1818)
                     /locus_tag="PKK95_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOC19785.1"
                     /translation="MDDDDTRFQIDIRLAAVPLAACATAGAVALLRRHCLGFVFQGFN
                     LLARTTAVENVELPLLYRGVGLKERRGAARDALKLVGLAGWEAHTPAELSGGQQQRVA
                     IARALVTNPLVLLADEPTGNLDTQRTREIHGAAVVAEP"
     gene            2297..>2957
                     /locus_tag="PKK95_16065"
     CDS             2297..>2957
                     /locus_tag="PKK95_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19786.1"
                     /translation="MRLRLAGLLVAALLVFTYRGTGIYLRYFSADLSSTLPLNVARTG
                     EHLVIQAVDENAPCFSAWPDSQVPKPGDRVLEIYDHEGRGGAVDGMFDYGAYIKPINV
                     SSPWTLVVERPGPGGPHRLRLAMPPSTPLVWSPATWLVNLAVDFYLPFLAAVTGLLIG
                     LARPRDNQAYLASLLFMALALTLGSHVGQYPPLIREVSLLVGVTGSSFLPYLVFLFFL
                     RF"
BASE COUNT          434 a         1050 c          956 g          517 t
ORIGIN      
        1 tctgtacgcc gttcgcgatt gcgattccaa acggggatcc aggtcgagga ttgaatcgga
       61 atcgggattg tcattcgtgc cgcagcgcct cgatcgggtc gagccccgcc gcgcgcctgg
      121 ccggcaggta gccgaacagc acccccatcg ccgccgagaa gaagaaggcg atcaggttga
      181 tctgcgggtt gaagacgtag ggcagcccca tcacccgcgt cagcgccacc gacgccgtcg
      241 tggccagggc gaggccgatc agcccgccga acgacgacag ggtcaccgcc tccaccagga
      301 actgcagcag cacctcgcgc tcgagcgccc cgatcgccag ccgcgttccg atctcgcgcg
      361 tccgctcggt caccgacacc agcatgatgt tcatgatccc gatgccgccc accagcaggc
      421 tgacggccgc caccgcgccg aggagcgcgg tgagaatctg cgtgctgccc gacatggcgg
      481 tggcgatctc ccgggtgtcg aggatgttga agtcgtcgtc ctcgccctcc tcgatccggc
      541 ggcgctcccg catcagcgat tccaggcgcg ccttcagggc ggcggtgtcc accccgtcct
      601 tcgccgagat catcatcgac gagatgttct ggttgcccgc cagccgccgc tgcacggtct
      661 tgagcggcat cacgatcacg tcgtcctggt cgcgtcccat cgacgcctgg cccttcgact
      721 tcagcaggcc gatgacctcg cacgagaagt ccttgacccg gatggcgctt cccacgggat
      781 tctggccctc gaacagcttc gtccggatcg tctcgccaac cacgcagacc gggctgcccg
      841 tcgccatctc cgcgtcgctg aaggtccgtc ccgaggccaa ggtccagttg gcggccgtga
      901 agtagtcgtt gttcgagccg gtcaccgagg tggaccagtt cctcgccatc cacacggcgt
      961 cgccgaggct ccgctcgtcg gcgccagggc cttgatgccg ccgacctgct cctcgatgac
     1021 ccgcacgtcg gccaccttga acgacgcggc ggtcgtgcgg tcacggttcg gcccgggccg
     1081 ctggccgggc atcagcaccg gttgctgccc aggctcgcga tctgatcggc gaccgccttg
     1141 gtggccccgt ttcccagcgt caccatggtg accaccgcgg cgacgccgat gacgatgccg
     1201 aggatggtca ggaaccgacc gcatcaggtt gcggcgcatt tccttcaacg ccagctgcac
     1261 ggtgttccac agcattacga cacctcttcc tgcaccgcgt cgctctcgac gcgcccgtcg
     1321 aggaagtgga cgacgcgctg ggcgtaggcc gccgcctggg gttcgtgcgt cgccatcagg
     1381 atggtcagcc cccgttcacg gttcagcgac gacagcagct ccatggatct cccgcgtgcg
     1441 ctgggtgtcg aggttgccgg tcggctcgtc ggccagcagc acgagcgggt tggtcaccag
     1501 tgcccgcgcg atggccacgc gctgctgctg gccgcccgac agttcggcgg gcgtgtgcgc
     1561 ctcccaccct gccaggccga cgagcttcag cgcgtcgcgg gccgcccccc gccgttcctt
     1621 caggccgact ccgcggtaga gcagcggcag ctcgacgttc tcgacggcgg tggtgcgcgc
     1681 cagcaggttg aaaccctgga acacgaaccc gaggcagtgc cggcggagca gcgccaccgc
     1741 tcccgcggtc gcacatgcgg cgagcggcac ggccgcgaga cgaatgtcaa tctgaaaacg
     1801 cgtgtcatcg tcgtccaatc ttcctgtctc gattagacga cggcgtgtcc gggcgtgcgg
     1861 taaagagtgg tcggcgaact gtaaaggact gtcagcgtgc gatcggcaac gtgacaatca
     1921 cctcgaggcc gccgccggcc gcgttgcgag ccttgacggt cccgccttgc gaggcgatgg
     1981 cgcgctgggc gatcgagagg ccgaggccga ttccacccga ggcgccatcg gggaaagcat
     2041 agtgcagctc tccctattca agggtcctcc cgggcctctg gcggcaaaga ctcgtcgccg
     2101 gcgtgtagag aagtgtcaga gccgaaggcc ccgccgtccc ggccgtggcc aggaaccttc
     2161 cttgcttgtg cccgtagtac ctcacatacc gacagcgcca gaagctcgag ttcgctttgg
     2221 gtccggagcc gggatcgcga gccgcctcgc gttccctggg cccttggagc tgtggcgcaa
     2281 gccgtgagtc gtccctatgc gcctgcgtct agccggtctg ctcgtcgccg ccctgctcgt
     2341 cttcacctat cgcgggaccg ggatctacct gcgctacttc tcggccgacc tcagctcgac
     2401 gctgccgctg aacgtggccc ggaccggcga gcacttggtt attcaggccg ttgacgagaa
     2461 cgctccctgc ttcagcgcct ggcccgacag ccaggtgccg aagcccggcg accgggtgct
     2521 cgagatttac gaccacgagg gccgtggcgg cgcggtcgac gggatgttcg actacggcgc
     2581 ctacatcaag ccgatcaacg tgtccagtcc gtggacactc gtggtggaac gtcccgggcc
     2641 gggcggcccc catcgcctga ggttggcgat gcctccttcg acgccgctgg tgtggagccc
     2701 cgccacgtgg ctcgtcaacc tcgcggtcga tttctacctg ccgttcctgg cggcggtgac
     2761 cggcctgctg atcggcctgg cccggccgcg ggacaaccag gcctacctgg cctcgctcct
     2821 gttcatggcg ctggccctca cgctgggttc gcacgtcggg caatacccgc cgctcatccg
     2881 cgaggtgtcg ctcctggtgg gtgtcacggg ctcgtcgttc ctgccgtacc tggtgttcct
     2941 gttcttcctg cggttcc
//
LOCUS       DAOPQX010000420         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_36635_length_2943_cov_2.986842, whole genome shotgun sequence.
ACCESSION   DAOPQX010000420 DAOPQX010000000
VERSION     DAOPQX010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36635_length_2943_cov_2.986842"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..238
                     /locus_tag="PKK95_16070"
     CDS             95..238
                     /locus_tag="PKK95_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19787.1"
                     /translation="MITERLGAGMLLRYSAATIKVPMPGGQTRGKNGVGGLQFGVGGR
                     LRF"
     gene            254..1441
                     /locus_tag="PKK95_16075"
     CDS             254..1441
                     /locus_tag="PKK95_16075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC19788.1"
                     /translation="MTDRKLNVLHVCDHLGWEGSRMHGVKRLFAWMIPRFDPARFDVS
                     LVSLRKRDLSEETLDSFGVDITYLGKSKFDPTTLAALLRVMDRKRTDILHLHGYGATT
                     FGRAAAALRRIPAVLHEHANLTDTPWFQKVADRLLEPFTDLAIAVSRSTADFVIGPRQ
                     VPARKVRVVYLGAPLDEFSRPRTAAEIAEARRDIGALDGDVVVGTITRLHDSKGNEYL
                     VEAARQVLGRRPAARFFLAGEGPLRPSLEAQAREAGLGDRFVFLGFVRDVARLLSAFD
                     IDVFPSLWEGTPLTVFEALGAGKAIVATDADGLVDVLADGNDALIVPKRDPAALADRI
                     VRLIDDPAERARLSSAARATAARYDIGAFVRKMERLYELLHEVSRATRRKGVLQADLS
                     FLD"
     gene            1564..>2943
                     /locus_tag="PKK95_16080"
     CDS             1564..>2943
                     /locus_tag="PKK95_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19789.1"
                     /translation="MKTEHLVAVVLLVLLLSAAFTVDPVHNGWGLKGDEATYTAMTLS
                     AAYDGDLAFERKDLERYWAVYTCGPDGSFLKRGKMVRLKLVGGWPFVRVMRWGDAPLD
                     RLYFGKAYIYPVFAAPFVRLLGLRGMLVFHVLLLAGIAWLGYRFLAARSPRAVAFTFA
                     LAFLGASVVPVYAVWLTPEIFNLSLVFYAYFLWLYKQVAPPSERPGRVGRFVRGPWSD
                     VAAVALLGMVTFSKPLYVLLAGPVIAWPLWGAWRRGEGAGFLGRLGRAAALGALFAGV
                     AGGLFAINAAVTGELNYQGGNRRVFYSNFPHQTPAMTFETVGYPTATNELDTSAVLDR
                     RDLAERFARNAVYFVVGRHAGLLPYFFPGLVALVLWWWRWREIRGWHVLALLAVLATI
                     AVTLAILPYTWSGGGGPFGNRYFLPAYAVLFFFLPPVQSLAAPLAAWAGGALFTAQLV
                     LNPFFTARYP"
BASE COUNT          364 a         1045 c         1037 g          497 t
ORIGIN      
        1 ggagcggcgt cggcggcaac atcaccggcg acctgaccta catgatcacc gagcgcctcg
       61 gtgcggggat gctgctgcga ggcgacctga ccacatgatc accgagcgcc tcggtgcggg
      121 gatgctgctg cggtactcgg ccgccaccat caaggtgccg atgcccggag ggcagacacg
      181 cggcaagaac ggcgtcggcg ggctgcagtt cggcgtgggc gggcgcctcc gcttctagcc
      241 cggctcgttg ccgatgaccg accgcaagct gaacgtcctg cacgtgtgcg accacctggg
      301 ttgggaaggg tcgcgcatgc acggcgtgaa gcgcctcttc gcctggatga tcccccggtt
      361 cgatccggcg cgcttcgacg tgtcgctggt cagcctgcgg aagagggacc tctccgagga
      421 gacgctcgac agcttcggcg tggacatcac ctacctcggg aagtcgaagt tcgacccgac
      481 cacgctcgcg gccctcctcc gggtgatgga ccggaagcgc accgacatcc tgcacctgca
      541 cgggtacggc gcgaccacct tcggacgggc cgccgccgcg ctccggcgga tccccgccgt
      601 gctgcacgag cacgcgaacc tcaccgacac cccctggttc cagaaagtgg ccgatcgcct
      661 gctcgagccg tttacggacc tggcgatcgc ggtgtcccgc agcaccgccg acttcgtgat
      721 cgggccgcgg caggtgccgg cgcgcaaggt gcgggtggtc tacctcggcg ccccgctcga
      781 cgagttcagc cgcccgagga cggcggccga gatcgccgag gcccggcgcg acatcggcgc
      841 cctcgacggc gacgtcgtgg tgggcaccat cacgcggctg cacgactcga aggggaacga
      901 gtacctggtg gaggccgcgc ggcaggtgct cggccgacgc cccgcggccc ggttcttcct
      961 cgccggggag gggccgctgc ggccgtcgct cgaggcgcag gcgcgcgagg cggggctggg
     1021 ggatcggttc gtcttcctgg ggttcgtgcg cgacgtcgcc cggctgctgt cggccttcga
     1081 catcgacgtg ttcccgtcgc tctgggaggg cacgccgctg accgtcttcg aggcgctggg
     1141 ggccggcaag gcgattgtcg ccaccgacgc cgacggactg gtggacgtgc tggccgatgg
     1201 gaatgacgcg ctgatcgtgc cgaagcgcga cccggccgcg ctggccgacc ggatcgtccg
     1261 cctgatcgac gatcccgccg agcgcgcgcg gctctcgtca gccgcgcggg cgaccgccgc
     1321 ccgctacgac atcggcgcgt tcgtgaggaa gatggaacgc ctctacgagc tgctgcacga
     1381 ggtgtcgcgg gcgacccgcc gcaagggcgt gctgcaggcg gatttgtcgt tcctcgatta
     1441 ggggccccgg ggagccccgc gctcccagta gcacggggct tcagccccgt ttctgccgga
     1501 gcgaacctcg ttggccagtg agctccgggg cagtggcaca ggcgtctcgc ctgtgaggga
     1561 gtaatgaaga ccgaacactt ggtggctgtc gtcctgctcg tgctgttgct gtcggcggcc
     1621 ttcaccgtcg acccggtgca caacggctgg gggttgaagg gcgacgaggc cacctacacc
     1681 gcgatgacac tgagcgccgc gtacgacggc gacctggcgt tcgagcgcaa ggatctcgag
     1741 cgctactggg cggtgtacac ctgcggcccc gacggctcct tcctcaagcg cggcaagatg
     1801 gtccgcttga aactggtggg cggctggccc ttcgtccggg tgatgcggtg gggcgacgcg
     1861 ccgctcgacc gcctctactt cggcaaggcg tacatctatc cggttttcgc cgctcccttc
     1921 gtgcggctgc tcggcctccg cggcatgctg gtcttccacg tgctgctgct cgcggggatt
     1981 gcctggctcg ggtaccgctt cctcgccgcc cgctctccca gggcggtggc cttcaccttc
     2041 gccctggcgt tcctgggcgc ctcggtggtc ccggtctacg ccgtctggct cacgcccgag
     2101 atcttcaacc tctcgctcgt cttctacgcg tacttcctct ggctctacaa gcaggtcgcg
     2161 cctccatccg agcgccccgg gcgcgtcggc cgcttcgtgc gcggcccgtg gagcgacgtg
     2221 gcggcggtcg cgctgctggg catggtcacc ttctcgaagc cgctctacgt gctgctggcg
     2281 gggccagtga tcgcgtggcc gctgtgggga gcctggcgcc gaggagaggg agccggattc
     2341 ctcgggcgcc tcgggcgcgc cgcggcgctc ggcgccctct tcgcgggggt ggccggcggc
     2401 ctcttcgcca tcaacgccgc cgtgacgggg gagctcaact accagggcgg caaccgccgc
     2461 gtcttctact cgaacttccc gcaccagacg cccgcgatga cgttcgagac ggtcgggtac
     2521 cccacggcga ccaacgagct cgacaccagc gccgtgctcg accggcgcga tctggctgag
     2581 cgtttcgccc gcaacgcggt ctacttcgtc gtcggccggc acgccgggct gctgccgtac
     2641 ttcttccccg gcctcgtggc gctggtgctg tggtggtggc ggtggcgtga aatccgcggc
     2701 tggcacgtgc tggcgctgct cgcggtgctc gcgacgattg ccgtgacgct ggccatcctg
     2761 ccgtacacct ggtcgggcgg cggtggaccc ttcggcaacc gctacttcct gccggcctac
     2821 gcggtgctgt tcttcttcct gccgcccgtg cagtcgctcg ccgccccgct ggcggcctgg
     2881 gccggcggcg ccctcttcac ggcgcagctc gtgctgaacc cgttcttcac cgcgcgctac
     2941 ccg
//
LOCUS       DAOPQX010000421         2932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_36804_length_2932_cov_3.298575, whole genome shotgun sequence.
ACCESSION   DAOPQX010000421 DAOPQX010000000
VERSION     DAOPQX010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36804_length_2932_cov_3.298575"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PKK95_16085"
     CDS             <1..819
                     /locus_tag="PKK95_16085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOC19790.1"
                     /translation="DPGNPDFHTRTIPKVVGGVNPESTEAAVALYAAAIEKVVPVSSA
                     RVAESTKLLENIFRSVNIALVNELKMTFDRMGIDIWEVIEAARTKPFGFKPFYPGPGL
                     GGHCIPLDPFYLSWKAAEYGMWTRFIELAGEVNTHMPHYVVEKTIKALNEDGKSVRNA
                     RVLVLGLSYKEDIDDDRESPSYEIIELLQEAGAEVSYCDPYFPKARPGRKHKLDLASI
                     ACTPEAFAGYDAVLVSTGHRQFKDPALYSGAPLVIDTRNVVRLADGAKTRLVKA"
     gene            complement(919..1677)
                     /locus_tag="PKK95_16090"
     CDS             complement(919..1677)
                     /locus_tag="PKK95_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPLPA-CTERM sorting domain-containing protein"
                     /protein_id="HOC19791.1"
                     /translation="MKHRVLIGVVALLLAAPGAFADTVNPFGTDTGWHSWDTAGLYRT
                     GPPVTGQPWPWNNTSNDAGDCNVGYWVSGAAGCDVPGFYTSTDPAGYTPNYLGDGGTM
                     FMFEPTGQPITITVEQQVALWAKTGENVFGWFSATTGPTPLFSGSTPLGTVVTFVPDG
                     EWGFYITSPRGTFQTNTLDGHGKSHFAVFQVDPLGGYIIGFEDNLLGMGGDFDYNDAI
                     IRMAPVPVPEPASLLLLGAGLAGMAALRRRRPAR"
     gene            complement(1910..>2932)
                     /locus_tag="PKK95_16095"
     CDS             complement(1910..>2932)
                     /locus_tag="PKK95_16095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOC19792.1"
                     /translation="TPSLASQYARDAVRSVPQNPESHEMLVRALMLKGDLEGAAAALT
                     PLQVQHGSSARVKSLAGHLALARGDRAAARTAFEQALEASPRDNDALSGLVGLDLQDG
                     RPAAARQRLESRLKATPGDPAALAMAGRLYGQLGLVRESERAWKRLLEVDASNLQAYA
                     ALARLYLQQHRETEALAEAEGLARKNPGSVAAHTMAGFLLEALNRPAEARHRYERALA
                     LDAKAVVAANNLAWLLAQGGENLDVALQLAQTAKAGQPGSPEIDDTLGWVYYRKGMYD
                     LAVPPLEQAARSSPGRGTYHYHLGLAYAATKQGDRARASLQRAIELGLPPEAGADARR
                     VLAGLR"
BASE COUNT          452 a         1030 c         1002 g          448 t
ORIGIN      
        1 gacccgggca accccgactt ccacacgcgg accatcccca aggtggtggg cggcgtgaac
       61 cccgagtcca ccgaggccgc cgtggccctc tacgcggccg cgatcgagaa ggtcgtcccg
      121 gtctcgtcgg cccgcgttgc cgaatcgacg aagctgctcg agaacatctt ccgctcggtc
      181 aacatcgcgc tggtcaacga gctgaagatg accttcgatc gcatgggcat cgacatctgg
      241 gaagtgatcg aggcggccag gaccaagccc ttcggcttca agccgttcta cccgggcccc
      301 ggcctcggcg gccactgcat cccgctcgac cccttctacc tctcctggaa ggccgccgag
      361 tacggcatgt ggacgcggtt catcgagctg gcgggcgagg tgaacacgca catgccgcac
      421 tacgtcgtcg aaaagacgat caaggcgctc aacgaggacg gcaagagcgt caggaacgcc
      481 agggtgctcg tgctcgggct ctcctacaag gaggatatcg acgacgaccg cgagtcgccc
      541 tcctacgaga tcatcgagct gctgcaggaa gccggggccg aggtgtcgta ctgcgacccg
      601 tatttcccga aggcccgccc cggccgcaag cacaagctcg acctggcatc gattgcctgc
      661 accccggagg cgtttgccgg atacgacgcc gtcctcgtct cgaccggcca caggcagttc
      721 aaggacccgg cgctgtactc gggggcgccc ctcgtgatcg acacccgcaa cgtcgtccgg
      781 ctggcggatg gcgcgaagac gcggctcgtg aaggcctgaa cgccgcggcc ttgccgcccg
      841 cgacgaccac cgcagaaaga gaaagggcgg gaccaggctc ccgccctttc gtttctgttc
      901 gacccgcgag tcgcggtgct accgggccgg gcggcggcgc cgcagcgcgg ccatgccggc
      961 caatcctgcg cccagcagca gcagcgaggc cggctccggc accgggaccg gcgccatccg
     1021 gatgatggcg tcgttgtagt cgaaatcgcc tcccatgccg agcaggttgt cctcgaagcc
     1081 gatgatgtac ccgcccagcg ggtccacctg gaaaacggcg aagtgcgact tgccgtggcc
     1141 gtcgagggtg ttggtctgga acgtgcccct cggtgaggtg atgtagaagc cccactcgcc
     1201 gtcggggacg aaggtcacga cggtgccgag cggcgtgctg ccgctgaaga gcggcgtcgg
     1261 cccggtggtc gccgagaacc atccgaacac gttttcgccg gtcttcgccc acaacgccac
     1321 ctgctgctcc acggtgatcg tgatcggctg gccggtgggc tcgaacatga acatcgtccc
     1381 gccgtcgccg aggtagttcg gcgtgtagcc cgccgggtcg gtgctggtgt agaagccggg
     1441 cacgtcgcag ccggcggcgc cgctgaccca gtaccccacg ttgcagtcgc ccgcgtcgtt
     1501 gctggtgttg ttccagggcc agggctggcc ggtgacgggc gggccggtgc gatacagtcc
     1561 ggcggtgtcc caggagtgcc agcctgtatc ggtgccgaac gggttcacgg tgtctgcaaa
     1621 cgccccgggc gcggcaagca gcaacgcgac cacgccaatc agcactcggt gtttcacggc
     1681 ggcccctttc aggccggcac gatggacggt gccggcgaca ccaactgcct cctcccggag
     1741 cagaagctgt gccgtagcgt ccggcgcgcc gccgcggacg gggtcttcag gctaagtgaa
     1801 gataagactg tgacttagcg ctacagagag agaggggaag tccgcctggt taagagaaaa
     1861 gtctgctcga ggtgggaaga ttgtctgatc ttcttccggc acggcagggc tagcgcagcc
     1921 ccgccagcac cctccgcgca tcggcgccag cctccggcgg gaggcccagc tcgatcgccc
     1981 gctggagcga cgcccgggcg cggtcaccct gcttcgtcgc cgcgtaagcc agcccgaggt
     2041 ggtagtggta agttccgcgg cccggtgacg atctggccgc ctgctcgagc ggcggaaccg
     2101 caaggtcgta catgcccttc cggtagtaca cccatcccag ggtgtcgtcg atctcgggcg
     2161 accccggctg cccggccttc gcggtctggg ccagctgcag ggcgacgtcg aggttctccc
     2221 cgccctgggc cagcagccac gcgaggttgt tggcggcaac gacggccttg gcgtcgaggg
     2281 cgagcgcccg ttcgtaacgg tgccgtgcct ccgccggccg gttcagcgcc tcgagcagga
     2341 agcccgccat cgtgtgcgcg gccaccgagc cggggttctt tcgcgcaagg ccctccgcct
     2401 cggccagcgc ctcggtctcg cggtgctgct ggaggtagag ccgcgccagg gcggcgtacg
     2461 cctgcaggtt cgaggcgtca acctccagca gtctcttcca cgcgcgctcc gactcgcgca
     2521 ccaacccgag ctggccgtac aaccgcccgg ccatcgcgag ggcagcgggg tcgccgggcg
     2581 ttgccttcag gcgagactcg aggcgctgcc tggccgccgc tgggcggccg tcctgcaggt
     2641 ccaggcccac gagccccgaa agggcatcgt tgtcgcgggg gctcgcctcg agcgcctgct
     2701 cgaacgcggt cctggccgcc gcccgatcgc cgcgcgccag cgccaggtgc ccggcgagcg
     2761 acttcacccg tgcggagctg ccgtgctgca cctgcagcgg ggtcagcgcc gcagctgccc
     2821 cctcgaggtc gcccttgagc atcagcgccc gcaccagcat ctcgtgcgac tcggggttct
     2881 gcggcacgct cctgacggcg tcgcgcgcgt actggctggc cagcgacggc gt
//
LOCUS       DAOPQX010000422         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_37297_length_2903_cov_4.175562, whole genome shotgun sequence.
ACCESSION   DAOPQX010000422 DAOPQX010000000
VERSION     DAOPQX010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37297_length_2903_cov_4.175562"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="PKK95_16100"
     CDS             <1..1617
                     /locus_tag="PKK95_16100"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOC19793.1"
                     /translation="PDLRRAIARIAAAAVVPNASHDAAASAARREAAEAAVLPVSLAF
                     RRNQLIVGEGLEVTPQIRLALDELRARHAPAVFGSQVAGSSLLFFLLVIAGLLVRGDP
                     SRQAAGRPRGGADARDAVFLAGGLVLAASLLRLWLVTVDLLLAGNPALSPMALALLFP
                     LAGVPMLTRFVLDERSAAAQLLVWSVAAGLMADHGVEVASLTLVSGLVGMWAVARCSS
                     RACLVRAGLRAGGAAGAAALAAALVAQLGPPATATATLLGGAGGFLSGLLVIALAPAI
                     EWLFGYTTRINLLEMISYEHPLLKRLIAEAPGTFQHSATVGVLADAGAAAIGADRLLV
                     RVGALYHDIGKIENGALFIENQRGPNPHDSLPPAESARIVRRHVTDGVKLVERYGLGD
                     QVAAFVREHHGTRRIVYFLERAKAAGLTVDEADYTYPGPRPASRETAVLMVADQAEAV
                     GRLKPGRDVDEYRATIAAAIEQLRADGQFDEAPITTADLRRMRDAMAEVLARMHHRRI
                     PYPGQEGGPPLADGGAGQPAASSAGGPAAS"
     gene            1695..>2903
                     /locus_tag="PKK95_16105"
     CDS             1695..>2903
                     /locus_tag="PKK95_16105"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOC19794.1"
                     /translation="MKMKRTLAALSVCAFALAAYTAVPRAEEGFWPYNNVPRATIKKK
                     FGFEVTDAWLKHLQLATIKFRGGTGSIVSPDGLVLTNHHIALGSLQRLSTPEKDLVKD
                     GFVALTRDQELKVPGLTLNVLQDIEDVTEKVNAGVSPGMKAADAVAARQKAIEAIQEE
                     SLKATGMQGEVVSLYAGAVYNLYRYKTYNDVRLVFGAEYQTGFYGGDPDNFTYPRFNL
                     DVGMFRLYENGKPASTPNYLRWSRAGSKEGELVFTTGHPGATQRLNTFAHLQYRRDAG
                     LPFSIARNAMREAAIKKWAALGPENARQAKGELFGVQNSLKNQRHQLKGLQDPALLAK
                     KEAEEKRLREGLAKNPQKQQELGDAWQLIEQSLQAARQLDAERNFIADAAGLNSQLFT
                     LARGLVRQAYS"
BASE COUNT          423 a         1057 c         1012 g          411 t
ORIGIN      
        1 cccgatctcc ggcgagccat cgcgcgcatc gccgccgccg cggtggtgcc gaatgcgtcg
       61 cacgacgccg ccgcctccgc ggcgcggcgc gaggcggccg aggcggccgt tctcccagtg
      121 tcgctggcct tccggcgcaa ccagctgatc gtcggtgaag ggctcgaggt cacgccccag
      181 atccggctgg cgctcgacga gcttcgggcc cggcacgccc cggccgtctt tggatcgcag
      241 gtggccggct cgagcctgct gttcttcctg ctcgtgatcg ccggcctcct cgtgcgaggc
      301 gacccttccc gccaggccgc agggcggccg cgcggcggcg ccgacgcgcg cgacgccgtc
      361 ttcctggccg gcgggctcgt gctcgccgcc tcgctcctcc gcctctggct cgtcaccgtg
      421 gacctgctgc tggcggggaa tcccgccctt tccccgatgg ccctcgcgct gctcttcccg
      481 ctggccggcg tcccgatgct cacgcggttc gtgctggacg agcggtcggc ggctgcccag
      541 ctgctcgtct ggtcggtcgc cgccggcctg atggccgacc acggggtcga ggtggcaagc
      601 ctgacactgg tgtcgggcct ggtgggcatg tgggcggtcg cgcgctgcag cagccgcgcg
      661 tgcctggtcc gggccggcct gcgtgcggga ggggccgcgg gggcggcggc cctcgcggcg
      721 gcgctcgtcg cccagctcgg cccgccggcg acggccacgg ccacgctcct cggcggggcc
      781 ggcggtttcc tcagcgggct gctcgtgatc gcgctcgcgc ccgcgatcga gtggctgttc
      841 ggctacacca cgcgaatcaa cctgctcgag atgatcagct acgagcaccc gttgctgaag
      901 cggctgatcg ccgaggcgcc cggcaccttc cagcactcgg ccaccgtcgg ggtcctggcc
      961 gacgcggggg cggccgcgat cggggccgac cgcctgctgg tgcgcgtcgg cgccttgtac
     1021 cacgacatcg gcaagatcga gaacggcgcc ctgttcatcg agaaccagcg cggcccgaac
     1081 ccgcacgact cgctcccgcc cgccgagagc gcccgcatcg tccgccgcca cgtgaccgac
     1141 ggcgtgaagc tggtcgagcg gtacggcctt ggcgaccagg tggcggcctt cgtgcgcgag
     1201 caccacggca ctcgccggat cgtgtacttc ctcgagcggg ccaaagcggc cggcctgacg
     1261 gtggacgagg ccgactacac ctatccgggg ccgcggcccg cctcccgcga aacggccgtg
     1321 ctgatggtgg ccgaccaggc cgaggcagtc ggccgcctga agccggggcg ggacgtcgac
     1381 gagtaccgcg cgacgattgc cgcggcgatc gagcagctgc gcgccgatgg ccagttcgac
     1441 gaagcgccga tcacgacggc cgacctgcga cggatgcgcg acgcgatggc tgaagtcctg
     1501 gcgcggatgc accaccgccg catcccgtac ccgggccagg aaggggggcc gccgctcgcg
     1561 gacggcgggg ccggtcaacc cgccgcatcg tccgccggcg gacccgcggc gtcttgacag
     1621 ggttcccccg ccaaccctac agtcggtggg gttgcccccg tccggtcctc tttctccctt
     1681 ttcctgttca cgccatgaag atgaagagaa cgctcgccgc gctgtcggtc tgcgccttcg
     1741 cgctggccgc ttacaccgcc gttccgaggg ccgaggaggg cttctggccc tacaacaacg
     1801 tcccaagggc gacgatcaag aagaagttcg gcttcgaggt gaccgacgcc tggttgaagc
     1861 acctccagct cgccacgatc aagttccggg ggggcaccgg ctcgatcgtc tcgcccgacg
     1921 gcctggtgct caccaaccac cacatcgcgc tgggatcgct gcagcgcctg agcacgcccg
     1981 agaaggacct cgtcaaggac ggcttcgtgg cgctgacgcg cgaccaggag ctgaaggtcc
     2041 cgggcctgac cttgaacgtg ctccaggaca tcgaggacgt caccgaaaag gtgaacgccg
     2101 gcgtgtcgcc ggggatgaag gcggccgacg cggtggccgc gcgccagaag gcgatcgagg
     2161 cgatccagga ggagtcgctg aaggcgaccg gcatgcaggg cgaggtcgtc tccctgtatg
     2221 cgggggccgt ctacaacctc tatcgctaca agacctacaa cgacgtccgc ctggtgttcg
     2281 gtgccgagta ccagaccggc ttctacggcg gcgaccccga caacttcacc tacccgcgct
     2341 tcaacctcga cgtgggcatg ttccggctct acgagaacgg caagcccgcg agcacgccga
     2401 actacctgcg ctggtcgcgc gcagggtcta aggagggcga gctggtgttc accaccggcc
     2461 acccgggcgc cacgcagcgc ctcaacacct tcgcccacct gcagtaccgc cgcgacgcgg
     2521 ggctgccctt ctcgatcgct cgcaacgcga tgcgcgaggc ggcaatcaag aagtgggcgg
     2581 cgctcggccc cgagaacgcc cgccaggcga agggcgagct cttcggcgtg cagaacagcc
     2641 tgaagaacca gcgccaccag ctcaaggggc tgcaggaccc cgcgctgctg gcgaagaagg
     2701 aggccgaaga gaagcggctg cgcgaggggc tcgcgaagaa cccgcagaag cagcaggagc
     2761 tgggcgatgc gtggcagctc atcgagcagt cgctccaggc cgcccgacag ctcgacgccg
     2821 agcgcaactt catcgccgat gccgcgggct tgaactcgca gctcttcacg ctggcgcgcg
     2881 gcctcgtcag gcaagcctat tcg
//
LOCUS       DAOPQX010000423         2887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_37545_length_2887_cov_4.816737, whole genome shotgun sequence.
ACCESSION   DAOPQX010000423 DAOPQX010000000
VERSION     DAOPQX010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37545_length_2887_cov_4.816737"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2845)
                     /locus_tag="PKK95_16110"
     CDS             complement(<1..2845)
                     /locus_tag="PKK95_16110"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC19795.1"
                     /translation="MPLSLFHPAVADWFNSSFGEPTRPQALGWPAIARGESTLILSPT
                     GSGKTLAAFLWAIDRVMFAPEPEPARRCRVLYVSPLKALAVDVERNLRAPIAGIANLA
                     ASRGEAYRVPAALVRTGDTPSADRARFAREPADILITTPESLYLMLTGRAREALASVE
                     TVIVDEIHALVATKRGAHLALSLERLEALADRRLQRIGLSATQRPLDEVARFLGGAER
                     RRPGSRPSRRAAPAKRREIDGQRISAGLHDEFAARGEVSYRPVTIVDARQPKKLALKV
                     EVPVEDLARLAKPVEIPSGPAAQGPAAPSIWSSIHPRLLEHVRAHRTTLIFSNSRRLA
                     ERLAAAINELAGETIARAHHGSLAREQRVEIEDALKAGRIPALVATSSLELGIDMGAV
                     DLVIQIEAPPSVASGMQRIGRASHQVNAVSEGIIFPKYRGDLVAAAAVTRAMHRGQVE
                     ATRYPRNPLDVLAQQIVAAVAVEAWDVEELFATVRCAAPYASLSRAAFEGVLDMLSGR
                     YPSDDFAELRPRVTWDRLAGRLTARQGASRVAIANAGTIPDRGLYGVFLAGAPKGTAR
                     VGELDEEMVFESHVGETFVLGASTWRIEEILHDRVLVSPAAGEPGKMPFWKGDRPGRP
                     LELGLEVGALVRELLEMPRPAALARLTREHDLDRLAAENLLAYLADQQAATRAVPTDR
                     TIVVERNRDELGDWRVCLLAPFGGQVLAPWAMVAEARVRELRDFDVETMWSDDGFVVR
                     FPDTDEPPDPLWLLPPPDEAERLLLARLGGTSLFAARFREVAARALLLPRRRPGARTP
                     LWQQRKRASDLLAVAARFGSFPAILETYRECLRDQFDVPALVEVLSRVANRTTRVVTA
                     DTDSPSPFAASILFSYTANFLYDGDAPLAERRAQALLVDQAQLRDLLGDIELRELLDA
                     AVIDEVEGQLQRTAPGYRARSEDGVHDLLLRIGD"
BASE COUNT          414 a         1041 c         1060 g          372 t
ORIGIN      
        1 ggtcgccgat ccgcagcagc aggtcgtgca cgccgtcctc gcttcgcgcg cggtaaccgg
       61 gagcggtacg ctgcagctgg ccctcgacct cgtcgatcac cgcggcgtcc agcagttcgc
      121 gcagctcgat gtcgcccagc aggtcgcgca gctgcgcctg gtccaccagc agtgcctgcg
      181 cgcggcgctc ggccagcggg gcgtcgccgt cgtagaggaa gttcgcggtg tacgagaaga
      241 ggatcgaggc ggcgaagggc gagggcgagt cggtatccgc cgtcacgacc cgggtggtgc
      301 ggttcgccac ccgcgagagc acctcgacga gcgcgggcac gtcgaactgg tcgcgcaggc
      361 actcgcggta ggtctccagg atcgccggga acgatccgaa gcgcgccgcg accgccagca
      421 ggtcggacgc gcgcttgcgc tgctgccaga gcggcgttcg cgcacccggc cgccggcggg
      481 ggagcagcag cgccctggcc gccacctcgc ggaagcgcgc ggcgaagagg gaggttcccc
      541 ccagccgtgc gagcagcagg cgctcggcct cgtcgggagg cggcagcagc cacagcgggt
      601 cgggcggctc gtcggtatcg gggaaccgga cgacgaatcc atcgtcggac cacatggtct
      661 cgacgtcgaa atcgcgcagt tcccgcacgc gggcctcggc caccatcgcc cacggcgcca
      721 gcacctggcc gccgaacggc gccagcaggc agacgcgcca gtcgcccagc tcgtcgcggt
      781 tccgctcgac gacgatcgtc ctgtcggtgg gaacggcgcg ggtcgccgcc tgctgatcgg
      841 caaggtacgc gagcaggttc tcggcggcga gccgatccag gtcgtgctcg cgcgtcaggc
      901 gggcgagtgc cgcgggccgc ggcatctcga gcagctcgcg caccagcgcc cccacctcga
      961 gtcccagctc gagcggccgg cccggccggt cgcccttcca gaagggcatc ttgccgggct
     1021 ctccggcggc gggagagacg agcacccggt cgtgcaggat ctcctcgatc cgccacgtcg
     1081 aggctcccag cacgaaggtc tcgccgacgt gcgactcgaa caccatctcc tcgtccagct
     1141 cgcccacccg cgcggttccc ttcggcgcgc ccgcgaggaa gacgccgtac aaaccccgat
     1201 cggggatcgt tccggcgttg gcaatcgcca cccgcgacgc gccctgccgc gcggtcagcc
     1261 tcccggcgag gcgatcccac gtcacgcgcg gccgcagttc cgcgaagtcg tccgacgggt
     1321 accggcccga cagcatgtcg agcacgccct cgaacgccgc gcgcgacaag gacgcgtagg
     1381 gagcggcgca gcgcaccgtg gcgaacagct cctccacgtc ccacgcctcg acggcgacgg
     1441 cggcgacgat ctgctgcgcc agcacgtcga gcgggttgcg cgggtagcgc gtcgcctcga
     1501 cctggccccg gtgcatcgcc cgtgtcaccg ccgcggcggc caccaggtcg ccccggtact
     1561 tcgggaagat gatcccctcg ctgaccgcat tcacctggtg gctcgcgcgg ccgatgcgct
     1621 gcatgccgct cgcaaccgac ggaggcgcct cgatctggat gacgagatcc accgcgccca
     1681 tgtcgatgcc cagctcgagc gacgaggtgg cgaccagcgc cgggatgcgc ccggccttca
     1741 acgcgtcctc gatctcgacc cgctgctccc gcgcgagcga tccgtggtgg gcgcgggcga
     1801 ttgtctcccc cgccagctcg ttgatcgcgg ccgcgagacg ctcggcgagg cggcggctgt
     1861 tcgagaagat gagcgtcgtg cggtgggcgc ggacgtgctc gagcagccgc gggtggatgc
     1921 tggaccagat agacggagcc gccggcccct gcgccgcggg accgctcggg atctcgaccg
     1981 gcttcgccag cctcgccagg tcctcgacgg gcacttccac cttcagcgcc agcttcttcg
     2041 gctgccgggc gtccacgatg gtgacgggac ggtaggagac ctcgcccctt gccgcgaact
     2101 cgtcgtggag gccggcggag atccgctggc cgtcaatctc gcggcgcttc gcgggggcgg
     2161 cgcggcgcga gggacgcgac cccggccggc gccgctcggc gccgcccagg aagcgggcca
     2221 cctcgtccaa cggccgctgg gtggccgaca gcccgatgcg ctgcagccgg cgatcggcaa
     2281 gcgcctcgag acgctcgagc gagagcgcaa ggtgggcgcc acgcttggtc gccacgaggg
     2341 cgtggatctc gtccacgatc accgtctcga cgctggcgag cgcctcgcgc gcgcggccgg
     2401 tcagcatcag gtacagcgac tccggtgtgg tgatcaggat gtcagccggc tcgcgggcga
     2461 agcgggcccg gtcggccgaa ggagtgtcgc cggtgcgcac cagcgcggcc ggcacacggt
     2521 acgcctcgcc ccgggaggcg gcgaggttgg cgatgccggc gatcggcgcc ctcaggttgc
     2581 gctcgacgtc cacggcaagc gccttgagcg gcgagacgta gagcacccgg caccgccggg
     2641 ccggctcggg ctcgggcgcg aacatcaccc ggtcgatcgc ccagaggaac gccgccaggg
     2701 tctttccgct gccggtgggg gagaggatga gcgtcgactc gccccgcgcg atggcaggcc
     2761 agccgagcgc ctgcggccgc gtcggttcgc cgaaggagga attgaaccag tcggcgacgg
     2821 ccgggtggaa gagggagagg ggcacgagga gatcttaatc cttgtcgtgc tggaggtgct
     2881 ggaggtg
//
LOCUS       DAOPQX010000424         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_37971_length_2861_cov_4.895937, whole genome shotgun sequence.
ACCESSION   DAOPQX010000424 DAOPQX010000000
VERSION     DAOPQX010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37971_length_2861_cov_4.895937"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1189
                     /locus_tag="PKK95_16115"
     CDS             200..1189
                     /locus_tag="PKK95_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOC19796.1"
                     /translation="MPAAGVGTLGLVDGDLVGLSNLQRQIVHGTSAVGEPKVQSAARR
                     LADLNPSVRVVPHEASLTRENAPDLLGGYDIVLDCTDNFATRYLVNDASVLAGQPYVY
                     GSVFRFEGQASVFAAPGGPCYRCLFPEPPPAGLVPGCDEGGVLGVLPGIVGCIQANEA
                     IKLIVGVGEPLVGRLVVIDALEMRFREVRLHRDPDCPVCGDHPRIRELQDYEEVCGTR
                     AASLRAADRDIGPLELKRRLDEGAPTVLLDVREPMEYRIVHLPGSVLVPLGELAERAG
                     DLDRGASYVVYCHHGVRSVGAVELLRSLGLEAWNLAGGIEAWTDEVDPTLPRY"
     gene            1306..1773
                     /locus_tag="PKK95_16120"
     CDS             1306..1773
                     /locus_tag="PKK95_16120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HOC19797.1"
                     /translation="MLRLSKKADYALIAMKHLALREGRGSSSAREIAEQYSIPVELMA
                     KVLQRLARRGLLSSQQGTRGGYQLARDSDSISLADVIQAIDGPLTVTACSAHSQRCGQ
                     FATCNVRDPLWKIRELILGALSSSTVYEMAVDDHRMGGRDRLAVSQAAALPPR"
     gene            1798..>2861
                     /locus_tag="PKK95_16125"
     CDS             1798..>2861
                     /locus_tag="PKK95_16125"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOC19798.1"
                     /translation="MNLPIYMDCHATTPVDPRVLEAMLPYFRERFGNAASRNHSFGWQ
                     AEEAVESARKQIAALIGATAREIVFTSGATESDNLAIKGAARMYREKGDHIITAVTEH
                     KAVLDTCKHLQKEGFQVTYLPVGRDGLIDLDRLRDAITPKTTVISVMAANNEIGVVQP
                     IAEIGRIARERGVLFHTDAVQAAGKIPFDVEAMNVDMASLSAHKMYGPKGVGALYVRR
                     RNPRVLLEPIIDGGGHERGMRSGTLNVPGIVGMGKASAIAAGEMAEEGARVAALRDRL
                     LAGLRGGLDEIYVNGSMERRLPGNLNVSFAYVEGESLLMGISDVAVSSGSACTSATLE
                     PSYVLKALGVGDELAHSSIR"
BASE COUNT          430 a          970 c         1023 g          438 t
ORIGIN      
        1 gggatggagg cgatgtcgac ctcgtcgtac tggccgagat cgcccgtgta ggtgcacggc
       61 acggcgccct tgtcgcgcat ccacgcgacg gccacggagg tgtccaggcc ccccgagaag
      121 gcgatgccca cgcgctcgcc gacgggcagg gattggagga ccttcgacat gggttccagt
      181 ctagtgagcc gcgatccggg tgcccgcggc gggcgtcggc acgcttggcc tggtggacgg
      241 ggacctggtg ggcctgagca acctccagcg gcaaatcgtg cacgggacga gcgcggtcgg
      301 tgagccgaag gtgcagtcgg cggcccgccg ccttgccgac ctcaatcctt ccgtccgcgt
      361 cgttccgcac gaggcgagtc tgacccgcga gaacgcgcct gatctcctgg gcggctacga
      421 catcgtgctg gactgcaccg acaacttcgc gacgcgctac ctggtgaacg acgcctcggt
      481 gctcgcgggc cagccttacg tctacggcag cgtcttccgc ttcgaggggc aggcctcggt
      541 cttcgccgcc ccgggcggcc cctgctacag gtgcttgttc cctgaaccgc cgccggcggg
      601 cctcgtgccg ggctgcgacg agggaggtgt gctcggcgtg cttcccggca tcgtgggctg
      661 catccaggcg aacgaggcga tcaagctgat cgtcggcgtc ggcgagcccc tggtcgggcg
      721 cctggtggtg atcgacgcgc tcgagatgcg cttccgcgag gtccggctac accgcgaccc
      781 ggactgcccg gtgtgcggcg accacccccg catccgcgag cttcaggatt acgaggaggt
      841 gtgcggaacc cgtgccgcgt ccctgcgtgc ggccgatcgc gacatcgggc cgctcgagct
      901 gaagcgccgc cttgacgagg gtgcgcccac cgtcctgctc gacgtccgcg agccgatgga
      961 ataccggatc gtccacctcc ccggctcggt actcgtgccc ctgggcgagc tggccgagcg
     1021 tgccggcgat ctcgaccggg gcgccagcta cgtcgtctac tgccaccacg gcgtgcgcag
     1081 cgttggcgcg gtcgagcttc tgcggagcct cggcctcgag gcctggaacc tcgccggcgg
     1141 catcgaagcc tggaccgacg aggtcgaccc gacgctgccg cgatactgag ggccgtcccg
     1201 gcccgacgcc agcccttccc cttttgcgac cagcccggcc cttgattgcg tgggccgttg
     1261 cgccgtaaaa tacgactctg gcggtcgtag tttaaggcag agcctatgct gaggttgtcg
     1321 aagaaggcgg attacgcgct gattgccatg aagcacctgg ctctgcgcga gggccgcggg
     1381 tcgtcgagcg ctcgcgagat cgccgagcag tactccatcc cggtggagct gatggcgaaa
     1441 gtcctgcagc ggctcgcgcg gcgaggcctg ctctcgtcgc agcagggcac gcgcgggggc
     1501 taccagctgg ctcgcgactc cgactcgatc tcgctggccg acgtcatcca ggcgatcgac
     1561 ggtccgctga ccgtcacggc ctgctcggct cacagccagc gctgcgggca gttcgcgacc
     1621 tgcaacgtgc gcgatccgct ctggaagatc cgcgagctga tcctcggggc cctgtcgagc
     1681 agcacggtct acgagatggc cgtggacgac caccgcatgg gcggccgcga tcgcctcgcg
     1741 gtgagccagg ccgccgcact gccgccgcgc tgagaacgtc accatccaga ggtcaccttg
     1801 aacctgccga tttacatgga ttgccatgcg acgacgccgg tcgacccgcg cgtcctcgag
     1861 gcaatgctgc cgtacttccg cgagcggttc gggaatgccg ccagccggaa ccactcgttc
     1921 gggtggcagg ccgaggaggc cgtcgagtcc gcccgcaagc agatcgccgc gctcatcggc
     1981 gcgaccgcgc gcgagattgt cttcacgagc ggggccaccg agtcggacaa cctggccatc
     2041 aagggcgcgg cccggatgta ccgcgagaag ggcgaccaca tcatcacggc ggtcaccgag
     2101 cacaaggcgg tgctcgacac ctgcaagcac ctccagaagg agggttttca ggtcacctac
     2161 ctgccggtcg ggcgcgacgg cctgatcgac ctcgatcggc tgcgcgacgc gatcaccccg
     2221 aagacgacgg tgatcagcgt gatggcggcg aacaacgaga tcggggtggt gcagccgatc
     2281 gccgagatcg ggcggatcgc gcgcgagcgg ggcgtgctgt tccacaccga cgcggtgcag
     2341 gccgccggga agatcccctt cgacgtcgag gcgatgaacg tggacatggc gtcgctctcg
     2401 gcgcacaaga tgtacggccc gaagggcgtc ggggcgctct acgtccgccg ccggaacccg
     2461 cgcgtgctcc tcgagccgat catcgacggc gggggccacg agcggggcat gcgctcgggc
     2521 accctcaacg tcccgggcat cgtcgggatg gggaaggcct cggcgattgc ggccggcgag
     2581 atggcggagg aaggggcgag ggtcgccgcg ctgcgcgacc ggctgctggc cggcttgcgc
     2641 ggaggcctcg acgagatcta cgtgaacggc tcgatggagc gccggctgcc cgggaacctc
     2701 aacgtcagct tcgcctacgt cgagggcgag tcgctgctga tggggatcag cgacgtggcg
     2761 gtgtcgtcgg ggtcggcgtg cacgtcggcg acactcgagc cgtcgtacgt gctgaaggcg
     2821 ctgggggtcg gcgacgaact ggcgcactcg tccatccgct t
//
LOCUS       DAOPQX010000425         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_37974_length_2861_cov_3.399857, whole genome shotgun sequence.
ACCESSION   DAOPQX010000425 DAOPQX010000000
VERSION     DAOPQX010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37974_length_2861_cov_3.399857"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="PKK95_16130"
     CDS             <1..1425
                     /locus_tag="PKK95_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOC19799.1"
                     /translation="PAVIFFGHGLEAKIRGERLAQRGLAVPALLDSVREGETLDEVKE
                     RLGAPARERWSVRSRRVRRATGSAGTTTHAIDPAVEARFVRLNITNPSYSGEPRARIY
                     EFEVYGPQGRENLAFGRPATGSIPCSPAEGPEKAVNGSVAGGEQDRWCSYDWPLYLQV
                     DLGTTRPVARFVVKHASAGGESDESDTREFNIQVSTDGKVFTTVETSSGAAFVEERTE
                     YRELIYFDGRRQARYLFVDGRVDNRSVRPLLDFEEDRAIFAGIFQFFYLRLAVFFGCL
                     GMFMYLFSGEMSNRTLHYWFLVPARREVLLAGKYAAGLVAASVIFGGGALLASAALIW
                     PQDPVAVQAYWNAGGLRHLFWYTTAAVLGCVGYGSVFLAVGLYIRNPIVPAAVLLAWE
                     GAAGVLPHVLQKASILYYLQSLCPVPAPFDDDVPTLIRLLAAPAAPASRPAAILGLLL
                     LTAFVLWIAARAVRRMQISYGSES"
     gene            1746..>2861
                     /locus_tag="PKK95_16135"
     CDS             1746..>2861
                     /locus_tag="PKK95_16135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOC19800.1"
                     /translation="MAWFGLGDRSFKGGVHPPDQKALTRDLPLEQMPDPELLLLPLLQ
                     HLGCPAVALVKKGDWVRQGQLLADAADGISAVVRASRAGTIAAIRAGGTAAGFPGQML
                     VLQPGPPPAPVEGEDPRETSPVSLPPLDPAAVTASEVVSRVRDAGVVGQGGAAFPTAV
                     KLAPPKDSKLDLLIVNGCECEPYLTRDFRLMVERPAALVEGSALAARTVGVTRVVIGV
                     ENNKPDAVAALRAAAAAPDRPAWTSGVSIEVLPLQTKYPQGAEKMLIEAATGRRVPPG
                     GLPMAVGAGIQNVGTILAIRDAVVDGQVQTDAVLTVSGRGIVRPANLRVPVGTPIADV
                     IAFCGGLRDDVARVVVGGPMMGIAQHDLAAPVVKACSG"
BASE COUNT          393 a          999 c         1033 g          436 t
ORIGIN      
        1 ccggcggtga tcttcttcgg ccacggcctc gaggcgaaga tccggggcga acggctcgcc
       61 cagcgcgggc tggccgtgcc ggcgctgctc gacagcgtca gggaaggcga aacgctcgac
      121 gaggtgaagg agcggctcgg cgcgccggcc cgcgaacgct ggtcggtgcg cagccgccgc
      181 gtccgccggg ccaccggcag cgcggggaca accacccatg ccatcgaccc ggcggtcgag
      241 gcgcgcttcg tccgcctgaa catcaccaat ccgtcgtaca gcggcgagcc gcgcgccagg
      301 atctacgagt tcgaggtcta cgggccgcaa gggcgcgaga acctcgcgtt cggccggccc
      361 gccaccggca gcatcccctg cagcccggcc gaggggcccg agaaggccgt gaacggcagc
      421 gtcgctggcg gggagcagga ccggtggtgc tcctacgact ggcccctcta cctgcaggtc
      481 gatcttggaa cgacacgccc ggtggcacgc ttcgtcgtga agcacgcgag cgccggcggc
      541 gagagcgacg agtcggacac ccgcgagttc aacatccagg tgagcaccga cggaaaggtc
      601 ttcaccacgg tcgagacctc ctcgggcgcc gcgttcgtcg aggagcggac ggagtaccga
      661 gaactgatct acttcgacgg gcgacggcag gcccgctacc tgttcgttga cggccgggtc
      721 gacaaccgga gcgtccgccc gctgctcgac ttcgaggaag accgggcgat cttcgccggg
      781 atcttccagt tcttctacct gcggctggcc gtcttcttcg gttgcctcgg gatgttcatg
      841 tatctcttca gcggggagat gtccaaccgc acgctgcact actggttcct cgtgccggcc
      901 aggcgagagg tgctgctcgc ggggaagtac gcggcggggc tggtcgcggc gtcggtcatc
      961 ttcggcggcg gagccttgct cgcctcggcg gcgctgatct ggccgcagga tccggtcgcg
     1021 gtccaggcct actggaacgc cgggggactg cggcacctgt tctggtacac gacggccgcc
     1081 gtgctcggct gcgtcggtta cggcagcgtg ttcctggccg ttggcctcta catccgcaac
     1141 cccatcgtcc cggcagccgt gctgctcgcg tgggagggcg ccgcgggggt ccttccgcac
     1201 gtcctgcaga aggcgagcat cctgtactac ctgcagtcgc tgtgtcccgt gcccgccccg
     1261 ttcgacgatg acgtgccgac gctcatccgg ctactggccg ccccggctgc tcccgcctcg
     1321 cgcccggctg cgattctcgg cctgctgctc ctcacggcgt tcgttctctg gattgccgcc
     1381 cgggccgtcc ggcgcatgca gatctcctac ggcagcgaaa gctgactggg gccctgcaag
     1441 gcgcgattcc cggcgaggtc gggggcgtgt taatgtgata gggcaggcac cgagccaagg
     1501 gttgctaccg tgctgccgcc tgcagccggc gcggccctga gcccctcctt ggtgcagacc
     1561 gacaccgaac gctcgacgca cagcgaaagc ggtgaccgac ggcccggtac gtgccctccc
     1621 ctcggtcggg cagccagctg aggctgccga ctgcatgttt cacgggacag tcatcctcaa
     1681 cgcggtgtag gtcacccgtg ccggcaggct gcgaggctgc gagtcgccct ccgccggagg
     1741 ttggaatggc ttggtttggc ttgggggaca ggagcttcaa gggaggggtc caccctcccg
     1801 accagaaggc cctgacccgc gatctcccgc tggagcagat gcccgacccc gagctgctcc
     1861 tgctcccgct gctgcagcat ctcggctgcc cggccgttgc cctggtgaag aagggcgact
     1921 gggtgcgcca ggggcagttg ctggccgacg ccgcggacgg catcagcgcc gtcgtacgag
     1981 cgtcccgggc cggcacgatc gcggcgattc gcgcgggtgg aacggcggcc ggttttcccg
     2041 gccagatgct ggtgctgcag ccgggcccgc ccccggcccc cgttgaaggc gaggacccgc
     2101 gtgagacgtc gcccgtgagc ctgccgcctc tcgacccggc ggccgtcacg gcctccgagg
     2161 tggtgtcgcg cgtccgggac gcgggcgtcg tcggccaggg gggtgcggcg tttccgacag
     2221 cagtcaagct cgcgccgccc aaggacagca agctcgatct gctgatcgtg aacggctgcg
     2281 agtgcgagcc ttacctgacg cgcgacttca ggctgatggt cgagcgaccc gcggcgctcg
     2341 tggagggctc ggcgctcgcc gcccggacgg tgggcgtcac cagggtcgtg atcggcgtcg
     2401 agaacaacaa gcccgacgcc gtggccgcgc tgcgggccgc tgccgccgcc cccgaccggc
     2461 cggcctggac gagcggcgtt tcgatcgagg tcctgccgct gcagaccaag tacccccagg
     2521 gcgccgagaa gatgctgatc gaagcggcga ccggccggcg cgttccgccg ggcggcctgc
     2581 ccatggccgt gggggcgggc atccagaacg tcgggaccat cctggccatc cgtgacgcgg
     2641 tggtggacgg acaggtgcag accgacgcgg tgctcacggt cagcgggcgc gggatcgtgc
     2701 gccccgccaa cctgcgcgtg ccggtcggca cgccaatcgc cgacgtgatc gccttctgcg
     2761 gcggcctccg cgacgacgtg gcgcgcgtgg tggtgggcgg cccgatgatg ggcatcgcgc
     2821 agcacgacct ggcggcgccc gtcgtgaagg cgtgctcggg c
//
LOCUS       DAOPQX010000426         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_38977_length_2806_cov_4.322428, whole genome shotgun sequence.
ACCESSION   DAOPQX010000426 DAOPQX010000000
VERSION     DAOPQX010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38977_length_2806_cov_4.322428"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKK95_16140"
     CDS             <1..307
                     /locus_tag="PKK95_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19801.1"
                     /translation="VTATVRFDNLTRQHQLSRTIDGRGEEPRVTEDEEEVRRWLTEFD
                     RLPLFNTAQLEPNMVYYVKVRAHARPRVSGGLFFWPWGRGGKTGFAPRFTYLGDDRG"
     gene            360..2627
                     /locus_tag="PKK95_16145"
     CDS             360..2627
                     /locus_tag="PKK95_16145"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOC19802.1"
                     /translation="MLTLRDVPPPTRRPAVRPRGRRPFRDNPLQILAVIGLLAAALGV
                     ALAFSGSWRYSPDFLTEFVLYALSVADLTMILALVFVLARNIIKMMVERRRGLPIARF
                     RSKLVGALLGMTLVPAVLVLLVGSELIRSNVDRWFNAPMEEIVASANRMASDYYSERQ
                     LLTSGTAARLSRALGAMPFPEGDPVAVRDVIQPEVAQQRVSMVEVYRVARGRSGPPVV
                     SSVVDVAVPSLPREYNRASADRLAERVAAGSREAHGPEPLGGGGQLLRAAEPIRARDG
                     TLAGVVVASDFLTGEMAARSRRMTDAYEAYNQLKVLRNPLLGVYLSFFLMVTLMILVS
                     ATWMGLYLAARITRPVQRLAAAARAIGAGHLDQRLEPETSDEFGSLVEAFNTMAAELS
                     ASRQNLERSARTLERQHQEVEARRRYIQTILDRITTGVISLDADDRVSTVNTAAARLL
                     GVEGDQAGLPVGELLGRPDLEPLAKLIAGARPGDADKPAQEVSLAREGRELDLAVAMA
                     TLHGQGGAIEGRLIVFDDITPLIRAQKVAAWREVARRLAHEIKNPLTPIQLCAERLAR
                     HFQGAPPPTRELVAECTRTIVGEVESLKGLVDEFSQFARMPAPRRIPTALPPLLADTL
                     GLYVGLFRDVTIETRFPADLPPVRVDPEQIRRVVINLVDNAIEAMDRRGRVVLEVQHA
                     AAERIVRLIVADNGPGIPEGEREKLFLPYYSTKRRGSGLGLAIVRRIVAEHGGTIEVT
                     SNAPRGTRFTIDLPA"
BASE COUNT          381 a         1021 c         1008 g          396 t
ORIGIN      
        1 cgtgaccgcc accgtccggt tcgacaacct gacgcggcag caccagctct cccggacgat
       61 cgacgggcgc ggcgaggagc cgcgcgtcac cgaggacgaa gaggaggtcc gccgctggct
      121 gaccgagttc gaccgcctgc cgctcttcaa caccgcgcag ctcgagccga acatggtgta
      181 ctacgtgaaa gtgcgggccc acgcccgccc gcgggtgagc ggcggcctgt tcttctggcc
      241 ctggggccgg gggggcaaga ccggcttcgc cccccgcttc acctacctcg gcgacgacag
      301 ggggtgacgg gccctgacgc tcttcgtccg ggccgcggtc tcccggcggc ctgcccgata
      361 tgctgaccct gcgggacgtt ccaccgccga cgcgacgccc ggccgtgcgg ccgcgcggcc
      421 gccgcccgtt ccgcgacaac cccctccaga tcctggcggt catcggcctg ctggccgccg
      481 cgctcggcgt cgcgctcgcc ttcagcgggt cgtggcgcta ctcgcccgac ttcctcaccg
      541 agttcgtcct ctacgcgctg tcggtggccg acctgacgat gatcctcgcg ctggtgttcg
      601 tgctggcgcg caacatcatc aagatgatgg tcgagcggcg gcgcgggctg ccaattgccc
      661 gcttccgctc gaagctggtc ggcgccctgc tcgggatgac gctggtcccc gcggtgctcg
      721 tgctgctggt gggcagcgag ctgatccgca gcaacgtgga ccgctggttc aacgcgccga
      781 tggaggagat cgtcgcctcg gccaaccgga tggcgtccga ctactacagc gagcggcagc
      841 tgctcaccag cggcaccgcc gcccgcctct cccgcgcgct cggcgcgatg ccgttccccg
      901 aaggggatcc cgtggcggtg cgggacgtga tccagcccga ggtggcgcag cagcgcgtga
      961 gcatggtgga ggtctaccgg gtcgcgcgcg gccgctcggg cccgcccgtc gtgtcgtcgg
     1021 tggtcgacgt ggcggttccc tcgctgcccc gcgagtacaa ccgggcctcc gccgatcggc
     1081 tggccgagcg ggtggcggcg ggcagccgcg aggcgcacgg gcccgagccg ctcggcggcg
     1141 gagggcagct gctgcgggcg gccgagccga tccgcgcgcg cgacggcacg ctggccggcg
     1201 tggtggtcgc ctccgacttc ctgaccggcg agatggcggc gcgctcgcgc cggatgaccg
     1261 acgcttacga ggcgtacaac cagctcaagg tcctcaggaa cccgctgctc ggcgtgtacc
     1321 tgtcgttctt cctcatggtc acgctgatga tcctggtcag cgcgacctgg atggggctgt
     1381 acctcgccgc gcgcatcacc cgtccggtgc agcgccttgc cgcggccgcc cgggcgattg
     1441 gcgcgggcca cctcgaccag cgtctcgagc ccgagacctc cgacgagttc ggatcactgg
     1501 tggaagcgtt caacacgatg gccgccgagc tgtcggcgag ccgccagaac ctcgagcgct
     1561 cggcgcgcac gctcgagcgg cagcaccagg aggtcgaggc ccgccgccgg tacatccaga
     1621 cgatcctcga tcgcatcacg accggggtca tctcgctcga cgccgacgac cgggtcagca
     1681 cggtcaacac cgccgctgcc cggctgctcg gcgtggaggg cgaccaggcg gggctcccgg
     1741 tcggggagct gctcggccgg cccgacctcg agccgctcgc gaagctgatt gcgggtgcgc
     1801 gccccggcga cgccgacaag ccggcgcaag aggtctcgct ggcccgcgag gggcgcgagc
     1861 tcgacctggc cgtggcgatg gccaccctgc acggccaggg aggggccatc gaggggcggc
     1921 tgatcgtctt cgacgacatc acccccctga ttcgcgcgca gaaggtcgcg gcgtggcgcg
     1981 aggtggcgcg ccgcctcgcg cacgagatca agaaccccct cacgccgatc cagctgtgtg
     2041 ccgagcggct tgcccgccac ttccaggggg cgccgccccc cacccgcgag ctggtggccg
     2101 agtgcacccg gacgatcgtc ggcgaggtcg agtcgctgaa agggctggtg gacgagttct
     2161 cccagttcgc gcgcatgccg gcgccgcgcc gcatcccgac cgcgctgccg ccgctgctcg
     2221 ccgacacgct ggggctctac gtcgggctgt ttcgcgacgt gacgatcgag acccggttcc
     2281 cggccgacct cccgccggtc cgggtggacc ccgaacagat ccgccgcgtc gtcatcaacc
     2341 tggtggacaa cgcgatcgag gcgatggacc gccgcggccg cgtcgtcctc gaggtgcagc
     2401 acgccgcggc cgagcggatc gtgcggctga tcgtcgccga caacgggccg gggattcccg
     2461 agggcgaacg cgaaaagctg ttcctgccgt attattcgac caagcggcgc ggcagcggcc
     2521 tcggcctcgc gatcgtgcgc cggatcgtcg ccgagcacgg cgggacgatc gaggtgacgt
     2581 cgaacgcgcc ccggggcacg cggttcacga tcgatctgcc cgcctgagcg gacggcagga
     2641 gaacagccct cccgaagggg cagcggtagc gggtacgggt gacatgcggc cgaggatcct
     2701 ggttgtcgac gacgagccgg gcgtgcggaa cgcgctctcc ggcatcctgc gcgacgaggg
     2761 ctaccacgtt gaagccgtcg agagtggcga ggactgcctc gaccgc
//
LOCUS       DAOPQX010000427         2804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_39016_length_2804_cov_4.717716, whole genome shotgun sequence.
ACCESSION   DAOPQX010000427 DAOPQX010000000
VERSION     DAOPQX010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39016_length_2804_cov_4.717716"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..962)
                     /locus_tag="PKK95_16150"
     CDS             complement(99..962)
                     /locus_tag="PKK95_16150"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOC19803.1"
                     /translation="MNNQPLAYFSREFLESDAGRPVRLLSEYLEPLRRFEQERIQDTV
                     VFFGSARTPSRARAEAALAALRQKSGGELAGRDEALVEQARRAVDWSRYYEDARLLAR
                     RLTAWSRALEAPFHRFVVCSGGGPGIMEAANRGAREAGGKTIGLNIRLPMEQGPNPYI
                     SPGLHFEFHYFFMRKFWFAYLAKALVVFPGGFGTLDEMFEILTLVQTEKLSRRIGVIL
                     YGREYWDRVLRLEPMVEAGAISPADLDLFSRADTPDEAFDILTGHLTEHVLRQEAPSD
                     QRAPKIARTRA"
     gene            complement(959..2323)
                     /locus_tag="PKK95_16155"
     CDS             complement(959..2323)
                     /locus_tag="PKK95_16155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOC19804.1"
                     /translation="MPDAVAPAEPRLPSRLYRWLVLLFISLAMFGNYYVYDCISPLAD
                     VLKAQLGFSDSSIGLLQAIYSFPNIVMVLVGGIIIDRVGTKRSTLLFGVICFLGAAIT
                     AWPNLPLFAPLAAGVRGLGSVFGFEVESQVLASMVAGRLVFGLGAESLIVAVTTAIAR
                     WFKGKELSFAFGMNLTIARLGSFAALNSPSWASGSYEHWRGPLLISVGFATFCVVGAL
                     LYWVLESRAEQRYALGSAGATDKVRFREIFDFGTSYWFIVALCITFYSGIFPFQTFAV
                     KFFMEAHGASRETAGFLSSMLTLFAMIGTPAFGYLVDRVGRRSLFMMFGSMLLIPVYL
                     MMAYTQISLYVPMAMMGVAFSLIPAVMWPSVAYVVGESKLGTAYGLMTMIQNIGLFAF
                     NLLIGWANDTWGASASNPAGYTPGMWMFSTLGFAGLLFAFLLRRSESGPRAHGLETIT
                     VQRA"
     gene            complement(2402..>2804)
                     /locus_tag="PKK95_16160"
     CDS             complement(2402..>2804)
                     /locus_tag="PKK95_16160"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOC19805.1"
                     /translation="GVVEEIKLRTIVLRDADGALHVFPNGTITTLANLTREFSYATAD
                     VVVSHGENLDRVMGALRMIGVGMQDDAELGGLLLGPLEMLGVQELRESSLTLRVRFRT
                     LPGKQWQVAAELRRRIAIGLAARGVKPFRSQ"
BASE COUNT          500 a          982 c          931 g          391 t
ORIGIN      
        1 cgcgagggcg cccgactgac gactcggccg acttcgtgga ctcgactccg agacttcgcg
       61 actgcgtgat ttcgtgactc cgtggactga ccgtcaggtc aggccctcgt cctcgcaatc
      121 ttgggcgccc gctgatcgga cggcgcttcc tgccgcagca cgtgctcggt aaggtggccg
      181 gtgaggatgt cgaacgcctc gtccggcgtg tcggcccgcg agaacaggtc gaggtccgcc
      241 ggggagatgg cgccggcctc caccatcggc tcgaggcgca gcacccggtc ccagtactcc
      301 cgcccgtaga ggatcacgcc gatgcgccgc gagagcttct cggtctgcac gagcgtgagg
      361 atctcgaaca tctcgtcgag cgtgccgaac cccccgggga agacgacgag cgccttggcc
      421 aggtaggcga accagaactt ccgcatgaag aagtagtgga actcgaagtg cagcccgggc
      481 gagatgtaag ggttcggtcc ctgctccatg gggaggcgga tgttcaggcc gatcgtcttg
      541 ccgccggcct cgcgggcacc gcggttggcg gcctccatga tgccgggccc gccgcccgag
      601 cagacgacga agcggtggaa gggggcctcg agcgcgcggc tccaggcggt gagccggcgt
      661 gccagcagcc gcgcgtcctc gtagtaccgc gaccagtcca cggcgcgccg tgcctgctcg
      721 accagcgcct cgtcgcggcc ggcgagctcg ccgcccgatt tctgccgcag ggcggccagt
      781 gcggcctcgg cgcgcgcgcg gctcggtgtc cgggccgatc cgaagaagac cacggtgtcc
      841 tggatgcgtt cctgctcgaa gcggcggagc ggctcgagat actccgagag gaggcggacc
      901 ggacgcccgg cgtcgctctc gaggaactcc cggctgaagt atgcgagcgg ctggttgttc
      961 atgcgcgttg aacggtgatg gtctcgaggc catgggcccg cggccccgac tcgctgcgcc
     1021 ggagcaggaa ggcgaacagc aggccggcga accccagcgt cgagaacatc cacatgccgg
     1081 gggtgtaccc ggccgggttc gaggcgctgg cgccccaggt gtcgttcgcc cacccgatca
     1141 acaggttgaa ggcgaacagc ccgatgttct ggatcatcgt catcaggccg taggccgtgc
     1201 cgagcttcga ctcccccacc acgtaggcca ccgacggcca catcacggcg ggaatgagag
     1261 agaaggccac gcccatcatc gccatcggga cgtacagcga gatctgcgtg taggccatca
     1321 tcaggtagac ggggatgagc agcatcgacc cgaacatcat gaagagcgag cgccggccca
     1381 cccgatcgac caggtacccg aaggcgggcg tcccgatcat cgcgaacagg gtcagcatgc
     1441 tcgacaggaa gccggccgtc tcgcggctgg cgccgtgcgc ctccatgaag aacttcaccg
     1501 cgaaggtctg gaacgggaag atcccggagt agaacgtgat gcagagcgcg acgatgaacc
     1561 agtacgacgt gccgaagtcg aagatctcgc ggaagcgcac cttgtcggtc gccccggccg
     1621 acccgagcgc gtagcgctgc tcggcccgtg actcgagcac ccagtagagc agcgcgccca
     1681 ccacgcagaa ggtcgcgaag cccaccgaga tcagcagcgg cccgcgccag tgctcgtacg
     1741 agcccgacgc ccagctcggc gagttgagcg cggcgaacga tccgagccgc gcgatggtca
     1801 ggttcatccc gaacgcgaag ctcagctcct tgcccttgaa ccaccgggcg atcgcggtgg
     1861 tcaccgccac aatcagcgat tcggcgccga ggccgaacac cagccggccc gccaccatcg
     1921 aggccagcac ctgcgactcg acctcgaagc cgaagacgct gcccagcccc cgcaccccgg
     1981 cggccagcgg ggcgaagagc ggaaggttcg gccaggcggt gatggcggcg ccgaggaagc
     2041 agatcacgcc gaacagcagc gtcgaccgct tcgtcccgac gcggtcgatg atgatcccgc
     2101 cgaccagcac catcacgatg ttcggaaagc tgtagattgc ctgcagcagg ccgatgctcg
     2161 agtccgagaa cccgagctgc gccttcagca cgtcggccag cgggctgatg cagtcgtaga
     2221 cgtagtagtt cccgaacatc gccaggctga tgaacagcag caccagccac cggtacaggc
     2281 gcgacggcag ccggggctcg gcgggcgcta cggcgtcggg catgaagcac tccctcttct
     2341 tgagtctcga aggcgaccca gacaggcgag gacgcctgtc gcactggcga ggacgcctct
     2401 cctattggct ccggaacggc ttgaccccgc gggcggcaag gccgatcgcg atccgccggc
     2461 gcagttccgc cgccacctgc cactgcttgc cgggcagcgt cctgaaccgc acccgcagcg
     2521 tcaggctcga ctcgcgcagc tcctgcacgc ccagcatttc gagcggcccg agcagcagcc
     2581 ctcccagctc cgcgtcgtcc tgcatcccaa cgccgatcat cctcaacgcg cccatcaccc
     2641 ggtcgaggtt ctccccgtgc gacaccacca cgtcggccgt cgcgtacgag aactcgcgcg
     2701 tcaggttcgc cagcgtggtg atcgtcccgt tcggaaagac gtgaagggcc ccgtccgcgt
     2761 cgcgcagcac gatcgtccgc agcttgatct cctccaccac gccg
//
LOCUS       DAOPQX010000428         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_39088_length_2800_cov_3.915483, whole genome shotgun sequence.
ACCESSION   DAOPQX010000428 DAOPQX010000000
VERSION     DAOPQX010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39088_length_2800_cov_3.915483"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..482
                     /locus_tag="PKK95_16165"
     CDS             162..482
                     /locus_tag="PKK95_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19806.1"
                     /translation="MDRERWSSYPAATEEERTLLAVLNGTAVRKTLPVVSTHGDERSV
                     EATFAVEAAEPFDGKAEVVVRLVFHGPAPADGERLAEDPWDLLDAFLREAEGEARRRA
                     SARR"
     gene            479..973
                     /locus_tag="PKK95_16170"
     CDS             479..973
                     /locus_tag="PKK95_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOC19807.1"
                     /translation="MTARRGSGPAADPLHLFHPAVAAWFRETFPEGPTEAQRLAWPAI
                     ARGENILLVSPTGTGKTLAAFLVALDAILIEEHGDLLLIRVSGSHFIWKMVRRLVGVL
                     VEVGRGGLTPEQAAGFLEERSPWPARLTAPASGLFLERVAYPGDPPAPPLRPAIEVRS
                     GEQG"
     gene            complement(1018..2727)
                     /locus_tag="PKK95_16175"
     CDS             complement(1018..2727)
                     /locus_tag="PKK95_16175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOC19808.1"
                     /translation="MSPSAFRSAACLAGIVAVAAGCGPRVDPADLVIRNGVVATMVAQ
                     NPRAEGAAVRGGRIVAVGTTREIQAYIGPGTEVIDLEGRFAMPGFIESHAHFTGVGAA
                     RMQLDLMKTTSWDEIVAMVAEAASKAKPGEWITGRGWHQEKWTRPPEPAVEGFPTHEL
                     LSRAAPDNPVFLTHASGHASIANAKAMQLAGITRATPSPRGGEILKDAKGAPIGVFRE
                     TGDALLESALRQAREARSAEEIEAELDRQVELADREFLSKGITTVHDAGASFETVERY
                     KRFAEAGRLGVRLYVMLNESNESLAANASKYRMVDAAGGHLTVRAVKRLADGALGSRG
                     AWLLEPYADAPSTGLNTYPIDQLAETARIAAGNDLQLCVHAIGDRANRETLNVYESAY
                     KEAGRKDMRWRVEHAQHIAAADIPRFGALGVIPSMQGIHCTSDAPYVLARLGEERAAE
                     GAYVWRKLMAGGATIANGTDAPVEAVDPLPGYHALVARRQKNGEVFYAGQALSRDEAL
                     AAYTLNGAFAAFEEKSKGSLTPGKYADITVLSADITQVPEGDIEKAEVLYTIVGGKVL
                     YKK"
BASE COUNT          368 a         1006 c          994 g          432 t
ORIGIN      
        1 cgccgcgtga agaccggccg cagcaacgcg ttcttcgtcg agatcaccga agggctcgcc
       61 gaaggggacg tcgtcctcct cgctcggtaa gggcgaggcg cggccccccc gaccctgggg
      121 agacgctccg cggtcatcgc gcgctgccta tactcccgtc cgtggaccgc gaacgctgga
      181 gctcctatcc cgccgcgacc gaggaagagc gcactctcct cgcggtcctg aacggaacgg
      241 ccgtgcgaaa gacccttccg gtcgtctcca cccacggcga cgaacgctcc gtcgaggcga
      301 cgttcgcggt cgaggcggcg gagccgttcg acgggaaggc cgaggtcgtc gtccggctcg
      361 tcttccacgg acccgcgccg gcggacggcg agaggctcgc ggaggacccg tgggacctcc
      421 tcgacgcgtt cctccgcgag gccgaagggg aggcgcggcg ccgggcttcg gcacgacggt
      481 gaccgcccgc cggggcagcg ggccagcggc cgacccgctc cacctctttc accccgcggt
      541 cgccgcctgg ttccgcgaga cgtttcccga aggcccgacc gaggcgcagc gcctcgcctg
      601 gcccgcgatc gcccgcggcg agaacatcct cctcgtctct ccgaccggaa cgggcaagac
      661 gctcgccgcc ttcctcgtcg cgctcgacgc gatcctgatc gaggaacacg gcgacctgct
      721 gctgattcgc gtcagcggct cgcacttcat ctggaagatg gtgcgccgcc tggtcggcgt
      781 gctcgtcgag gtgggacgcg gaggcctgac gcccgagcag gcggcagggt tcctcgagga
      841 gcggtcgccc tggccggcgc ggctcaccgc gccggcctcc gggctgttcc tggagcgggt
      901 ggcctacccg ggcgacccgc cggcgccgcc gctgaggccg gcaatcgagg tgaggagcgg
      961 agagcagggc tgaagcccgc gctcctcagc tgtcggctgt cagctgtccg ctgtcagcta
     1021 cttcttgtac agcacctttc cgccgacgat ggtgtagagg acctcggcct tctcgatgtc
     1081 gccctcgggc acctgcgtga tgtcggccga gaggaccgtg atgtcggcgt acttgcccgg
     1141 agtcagcgag cccttcgact tctcctcgaa ggcggcgaag gcgccgttca gcgtgtaggc
     1201 ggcaagggcc tcgtcgcggc tgagcgcctg gccggcgtag aacacttcgc cgttcttctg
     1261 ccggcgcgcg accagcgcgt ggtagcccgg cagcggatcg accgcctcga ccggcgcgtc
     1321 ggtgccgttg gcgatcgtcg cccccccagc catcagcttg cgccagacgt acgcaccctc
     1381 ggccgcgcgc tcctcgccca ggcgcgccag cacgtagggg gcgtcggagg tgcagtggat
     1441 gccctgcatc gaggggatga caccgagcgc gccgaagcgc gggatgtcgg cggcggcgat
     1501 gtgctgcgca tgctccaccc gccagcgcat gtccttccgc cccgcttcct tgtacgcaga
     1561 ctcgtagacg ttcagggtct cgcggttggc ccggtcgccg attgcgtgga cgcagagctg
     1621 caggtcgttg ccggcggcaa tccgcgcggt ctcggccagc tgatcgatcg ggtaggtgtt
     1681 gaggccggtg gacggtgcgt cggcgtaggg ctcgagcagc cacgctccgc gcgagccgag
     1741 cgcgccgtcg gcgagccgct tgaccgcgcg cacggtcagg tgcccgccgg ccgcgtccac
     1801 catgcggtac ttcgacgcgt tggccgcgag gctctcgttc gactcgttga gcatcacgta
     1861 gaggcgcacg ccgagcctgc cggcctccgc gaaccgcttg tagcgctcca ccgtctcgaa
     1921 cgacgcgccg gcgtcgtgga cagtcgtgat ccccttggag aggaactctc ggtcggccag
     1981 ctcgacctgg cggtccagct cggcctcgat ctcctcggcg ctgcgcgcct cccgggcctg
     2041 ccggagggca ctctcgagca gcgcgtcgcc ggtctcgcgg aagacgccaa tcggagcgcc
     2101 cttcgcgtcc ttgaggatct cgccgccgcg agggctgggc gttgcgcgcg tgatgccggc
     2161 cagctgcatc gccttggcgt tcgcgatcga cgcgtggccg ctcgcatggg tcaggaacac
     2221 cgggttgtcg ggcgccgcac ggctcagcag ctcgtgcgtc gggaagcctt ccacggccgg
     2281 ctcgggaggc cgcgtccact tctcctggtg ccagccgcgg ccggtaatcc actcgcccgg
     2341 ctttgccttc gaggccgcct cggccaccat cgccacgatc tcgtcccagc tggtggtctt
     2401 catcaggtcg agctgcatcc gggccgcgcc gacgcccgtg aagtgggcgt gcgactcgat
     2461 gaagccgggc atcgcgaacc ggccttccag gtcgatcacc tcggtgccgg ggccgatgta
     2521 ggcctggatc tcgcgcgtgg ttccgacggc gacgatcctc ccgccgcgca cggccgcgcc
     2581 ctcggcacgc gggttctgcg cgaccatggt cgcgacgacg ccgttgcgga tgacgaggtc
     2641 ggccggatcg actcggggac cgcagccggc ggccaccgcc acgatgccgg ccaggcaggc
     2701 ggctgagcga aaggcggaag gcgacatgtg ctggttctcc aggggcggga gggtcgattc
     2761 taccccaggg gcgtcagcag gtacaatcga cgcgatgcgc
//
LOCUS       DAOPQX010000429         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_39822_length_2760_cov_3.486876, whole genome shotgun sequence.
ACCESSION   DAOPQX010000429 DAOPQX010000000
VERSION     DAOPQX010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39822_length_2760_cov_3.486876"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PKK95_16180"
     CDS             <1..1136
                     /locus_tag="PKK95_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19809.1"
                     /translation="PVSLVIPMIPSELDRPALARILAELCEVRYLHSVVISLNKATRD
                     DYHRTLEYFAPYPGNTVVVWSESPAVQRFFEGLEQAGLEVGSPGKGRACWLAMGYLLA
                     EEKADYVAFIDADIANFSREMLARLILPVVDPILDFDFAKAYYARFSDRLHGRVTRLY
                     LSPLLAAFTRLIGDHTYIRYLSSFRYALSGEFAVQRDLASRMRLPSDWGLEIVSLFEV
                     LRHRAPVRLCQVEIADRYEHKHQDLSGEDPSRGLNRMARDVGTHLLRTLAAAGVVLSS
                     GLLESLMAAYQREAEDAVASSYAVAAINGLRHDRHQEELNVQTFASALRAAIEEFHKD
                     PLGPPLVPNWARVWAGLHEAGPQLLAAVAKGADGHASNGGGCR"
     gene            complement(1152..>2760)
                     /locus_tag="PKK95_16185"
     CDS             complement(1152..>2760)
                     /locus_tag="PKK95_16185"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOC19810.1"
                     /translation="IVVSRIAEDGRLETALESAGQAWGRFLRYEYLRLDFTRVTRPGL
                     YVVWYGRSRSNPFRIADDVYRRHVWQPTIDSFLPIQMCHVRVNDGYRVWHDACHLDDA
                     RMAPVDLNHFDGYMQGPSTLTRFAPGEPVPGLNRGGWHDAGDDDLRVESQADTMRGLA
                     LAWEAFHPRHDDTSIDQALRVVEMHRPDGKPDILQQLEHGALSVAGAYRSLGRLYRGI
                     ISPTLRQYTHLGDFAAQTDGIVHDPRTAAPANVPVGAGVPGSPDDRWVFTEQNPRREL
                     SAAAGLAAAARALKGYDDRLAGECLDIAKAIWEGTREDAATPGRPGGAGRPSPRIGLA
                     VELLLATGDRRYADYLLSERETIVKTFRSSGWIASRALGAVGDAAFTAAMTDAARAYR
                     AEVVKMAAKTPYGVPYEPDIWGAGWGIQRFGMEQYFLNAAFPEIFPREPMLHALDFVL
                     GFHPGPNNASFVSGVGSRSVTVGYGFNRADWGYIPGGSVSGTALIRPDFPELLEWPFL
                     WQQTEYVLGGGTTDYLLLALAADHAFR"
BASE COUNT          397 a         1005 c          938 g          420 t
ORIGIN      
        1 tcccggtgtc gctggtgatc ccgatgatcc cctccgagct ggatcgtccc gcgctcgccc
       61 ggatcctcgc cgagctgtgc gaggtccggt acctccactc ggtcgtcatc tcgctcaaca
      121 aggcgacgcg cgacgactac caccgcacgc tcgagtactt cgcgccctac cctggtaaca
      181 cggtcgtcgt gtggagcgag tctccggcgg tgcagcggtt cttcgagggg ctcgaacagg
      241 ccggcctgga ggtcgggtcg cccggcaagg gccgcgcctg ctggttggcg atgggatacc
      301 tgctcgccga ggaaaaggcc gactacgttg ccttcatcga cgccgacatc gcgaacttca
      361 gccgggagat gctggcgcgg ctcatcctgc ccgtggtgga tccgatcctc gatttcgact
      421 tcgccaaggc ctactacgcg cggttctccg accggctcca cgggcgcgtg acacggctct
      481 acctctcgcc gctgctggcc gccttcacgc ggctcatcgg cgaccatacc tacatccgct
      541 acctctcgtc gttccgctac gccctttcag gcgagttcgc cgtccagcgc gatctggcgt
      601 cgaggatgcg gctgccctcc gactgggggc tcgagatcgt gtcgctcttc gaggtcctgc
      661 ggcaccgcgc ccccgttcgc ctctgccagg tggaaatcgc cgaccgctac gagcacaagc
      721 accaggacct ctcgggggag gatccctcgc gcgggctgaa ccggatggcg cgcgacgtcg
      781 gcacccacct gctgcgcacg ctggccgccg ccggcgtcgt cctctcgtcc ggcctgctgg
      841 agagcctgat ggccgcctac cagcgcgagg ccgaagacgc cgttgccagc agctacgccg
      901 tcgccgcgat caacggcctg cggcacgacc ggcaccagga agaactgaac gtgcagacgt
      961 tcgccagcgc gctgcgcgcg gccatcgagg agttccacaa agaccccctc ggcccgccgc
     1021 tggtccccaa ctgggcgcgc gtctgggctg gcctgcacga ggcagggccc cagctgctcg
     1081 ccgccgtcgc caagggggcg gacgggcacg cgagcaacgg gggaggctgc aggtaggaaa
     1141 cggtctcgcc cctaccggaa tgcgtggtcg gcggcaagcg cgagaagcag gtagtcggtc
     1201 gtcccgcccc cgagcacgta ctcggtctgc tgccagagga acggccactc cagcagttcc
     1261 gggaagtcgg gccggatgag cgcggtcccg gacacgctgc cgcccgggat gtatccccag
     1321 tcggcccggt tgaagccgta cccgaccgtg accgagcgcg agcccactcc cgagacgaac
     1381 gaggcgttgt tcgggccggg gtggaatccc agcacgaaat cgagcgcgtg cagcatcggc
     1441 tcccggggga agatctccgg gaaggcggcg ttgaggaagt actgctccat gccgaagcgc
     1501 tggatccccc agccggcgcc ccagatgtcc ggctcgtacg gcacgccgta cggcgtcttc
     1561 gccgccatct tcaccacctc ggcccggtac gcgcgcgccg cgtcggtcat cgcggcggtg
     1621 aaggcggcgt cgccgaccgc accaagcgcc cggctcgcga tccaccccga cgaccggaag
     1681 gtcttcacga tcgtctcccg ttccgagagc aggtagtcgg cgtagcggcg atcgccggtt
     1741 gcgagcagca gctccaccgc gagcccgatc ctcgggctgg gacggcccgc accgccgggg
     1801 cgtcctggcg ttgcggcgtc ctcgcgggtt ccttcccaga ttgccttcgc gatgtccagg
     1861 cattcgcccg ccaggcggtc gtcgtatccc ttcaacgcgc gcgccgcggc ggccaggcct
     1921 gcagccgcgc tcagctcgcg gcgggggttc tgctccgtga acacccaccg atcgtcgggc
     1981 gacccgggca cccctgctcc cacgggcacg ttcgccggcg cggccgttcg cgggtcgtgg
     2041 acgatgccgt cggtctgcgc cgcgaaatcg cccaggtggg tgtactgccg cagcgtgggg
     2101 ctgatgatgc cccggtacag ccggccgagc gaccggtacg cccccgcgac gctgagggcg
     2161 ccgtgctcga gctgctgcag gatgtcgggc ttgccgtccg gccggtgcat ctccaccacg
     2221 cgcagcgcct ggtcgatgga ggtgtcgtcg tggcgggggt gaaacgcctc ccacgccagc
     2281 gcgaggccgc gcatcgtgtc ggcctgcgat tcgacccgca ggtcgtcgtc gccggcatcg
     2341 tgccagccgc cccggttcag cccgggcacc ggctcgcccg gggcgaagcg cgtcagggtg
     2401 gacggcccct gcatgtagcc gtcgaagtgg ttgagatcga ccggcgccat gcgcgcgtcg
     2461 tccaggtgac acgcgtcgtg ccacacgcgg tagccatcgt tgacgcgcac gtggcacatc
     2521 tggatcggca ggaacgagtc gatggtcggc tgccacacgt ggcgccggta gacgtcgtcg
     2581 gcaatccgga acgggttgga tcgggagcgg ccgtaccaaa cgacgtagag gcccggccgc
     2641 gtcacccgcg tgaagtcgag ccggaggtac tcgtagcgca ggaaccgccc ccacgcctgc
     2701 cccgccgact cgagcgccgt ctcgagcctc ccgtcctccg ctatccgcga cacgacgatc
//
LOCUS       DAOPQX010000430         2748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_40079_length_2748_cov_3.927961, whole genome shotgun sequence.
ACCESSION   DAOPQX010000430 DAOPQX010000000
VERSION     DAOPQX010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40079_length_2748_cov_3.927961"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..307
                     /locus_tag="PKK95_16190"
                     /pseudo
     CDS             95..307
                     /locus_tag="PKK95_16190"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing protein"
     gene            344..571
                     /locus_tag="PKK95_16195"
     CDS             344..571
                     /locus_tag="PKK95_16195"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HOC19811.1"
                     /translation="MEGRVRDLTGTCPIVRFRIRGTTITTSPGTRYGPDPPGAACGDL
                     RNGRTVTVTGLERDGGVEATLILFETNGSNP"
     gene            568..1770
                     /locus_tag="PKK95_16200"
     CDS             568..1770
                     /locus_tag="PKK95_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19812.1"
                     /translation="MRTTIERRAAAALAALVCVAGATGAAFAQQPEETVTVREALAFL
                     ITTQAVDTGDAARDRAAAVATSQTLSRALLSALATLPLTSSSAAFTYRFNPALGTLER
                     ASESFGPFFVERAMTAGRGRASFSVNWQYGRFTRLSDMALRDGTLVTTSNRFADEDEP
                     FDVETLELRISSSSITFSGSYGITDRVDVSAAVPVVSLSLEGQRTDLYRGTRFQQATA
                     TASVTRVADVLLRAKVNAAQGSWGALGVGGDLRLPTGREEDLVGAGSVGARGFAVLTA
                     GTGNVSGHLMAGVSRGGIAEGFDFGGAVSLNPKPTLTFAAELFARRLAEVGRVRQATS
                     PHPRIAGVQTLRLVQEEGSLTETHAAVGFKWNVGGMWLASATVVLPVNRAGLTAPLMP
                     SVSLEYSF"
     gene            complement(1956..>2748)
                     /locus_tag="PKK95_16205"
     CDS             complement(1956..>2748)
                     /locus_tag="PKK95_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19813.1"
                     /translation="SEPGPDLPRTVDLQPGGGSEVSPEGHVDLLGIGQQVLAQPGEPV
                     AAPSPAGVATSGQLASAGPSREVARPPASLPSAPASPPALTAGSSARPTPIAAPPPVA
                     AGIPGGAEPSPAPPSGEPAPFTAPLAGVAFSPPSTEPMPPPGDAAPAALRDVAADEEA
                     ISEVLGQYVDAYNRLDARAASRVWPTVDERALARAFAGLESQGITFEGCDLSVDDAEA
                     TAACRGRARHVPKVGERNPISHSRRWTFRLQRTDSGWQIMQAEAQ"
BASE COUNT          381 a          971 c         1026 g          370 t
ORIGIN      
        1 ggccaatccg agaagaactg tcagagggat tgcggtacta cgtgagagct aacagccggc
       61 gccgccgccg ccgtgcacgg cgtccgtctc gcctgcctcg gcttcgttca cttcgggcgg
      121 cggtcaggcc gaggtccagc tgtcgactgg cggggactgc gagtggacgg cggaatcgag
      181 cgagtcctgg ctgggagtgc agcccggttc gggcacgggc gacgcggtgc tcaggttgac
      241 ggccgcgccg aaccgcggca gcgcgacgcg gaatgcgacc gtgacgatcg cgggcgagcg
      301 cctgagcgtg acacaagagg gcgcgcccgt cgaggaagtg cgcgtggagg ggagggtccg
      361 cgacctgacc ggcacctgcc ccatcgtccg cttccggatc cgcggcacga caatcacgac
      421 ctcgcccggc acccggtacg gccctgatcc gcctggagcg gcgtgcggcg acctgcggaa
      481 cggccggacc gtcaccgtca ccggcctcga gcgggacggc ggcgtcgagg ccaccttgat
      541 cctcttcgag accaacggat ccaacccatg agaacgacga tcgagcggcg cgccgcggcg
      601 gcactcgcgg cactggtgtg cgtggcgggc gcgacgggag ccgcgttcgc ccagcagccc
      661 gaggaaaccg tgacggtgcg cgaggcgctg gccttcctga tcaccacgca ggccgtcgat
      721 accggcgacg cggcacgcga ccgcgcggca gcggttgcga cgagccagac gctgtcccgt
      781 gccctgctgt cggcccttgc cacgcttccg ctgacctcgt cctcggcggc cttcacctac
      841 cgcttcaacc ccgcgctcgg aaccctcgag cgggcgagcg agagcttcgg ccccttcttc
      901 gtcgagcggg cgatgacggc gggccgggga cgcgcctcgt tcagcgtgaa ctggcagtac
      961 ggccggttca cgcgcctctc cgacatggcg ctgcgcgacg gcaccctggt gacgacgtcg
     1021 aaccgcttcg cggacgagga cgagccgttc gacgtcgaga cgctcgagct gcgcatcagt
     1081 tcgagctcga ttaccttctc gggaagctac gggattaccg accgggtcga cgtgagcgcc
     1141 gccgtgccgg tggtctcgct gtcactcgag ggccagcgaa ccgatctcta ccgcggcacc
     1201 cgcttccagc aggcgaccgc gaccgcttcg gtcacgcgcg tggccgacgt cctgctgagg
     1261 gcgaaggtca acgccgcgca gggcagttgg ggcgcccttg gcgttggcgg cgacttgagg
     1321 ctgccgaccg ggcgcgagga agacctggtt ggcgccggct cggtcggtgc gcgcggcttc
     1381 gcggtgctca ccgcgggcac cgggaacgtg tcgggccacc tcatggccgg cgtcagccgc
     1441 ggcggcatcg ccgaaggctt cgatttcggc ggcgccgtgt cgctcaaccc caagcccacg
     1501 ctgaccttcg ccgccgagct gttcgcccgg cgccttgccg aggtcgggcg cgtgaggcag
     1561 gccacctcgc cgcacccgcg catcgcgggg gtgcagacgc tgcggctggt gcaggaggaa
     1621 gggagcctga ccgagacgca cgcggccgtg gggttcaagt ggaacgtcgg ggggatgtgg
     1681 ctggccagcg ccacggtggt gctgccggtg aaccgcgcgg gcctgacggc cccgctgatg
     1741 ccgtcggtgt cgctggagta cagcttctag cccgatccca gtcgtgcagg gggtgcaggt
     1801 ggtgcaaagg gtgcagcggg tgctggtgcg ggtggggcca aaggtgctgg tgctgggggt
     1861 gcaggcgcgg cactcgcgcc acaagcacct tcagcacctc cagcactgca ccttctgcac
     1921 cccctgcacc ccctgcaccc cctgcacgac tgggatcact gagcctccgc ctgcatgatc
     1981 tgccagcccg agtccgtgcg ctgcagccgg aaggtccacc ggcgcgagtg gctgatcggg
     2041 ttgcgctccc cgaccttcgg cacgtggcgg gcccggccgc ggcaggcggc cgtcgcttcc
     2101 gcgtcatcca ccgacaggtc gcagccctcg aaggtgatgc cctgcgactc gaggccggcg
     2161 aaggcgcgcg ccagcgcccg ttcgtccacg gtcggccaca cccggctggc cgcccgggcg
     2221 tcgaggcgat tgtaggcatc cacgtactgc cccagcacct cgctgatggc ttcctcgtcg
     2281 gccgccacgt cgcggagtgc cgcgggggca gcgtcgccgg ggggcggcat cggctctgtg
     2341 gaaggcggcg agaaggccac cccggccaga ggcgccgtga aaggtgccgg ctcccccgag
     2401 ggcggtgcag gcgagggctc cgccccgccg gggataccgg ccgcgaccgg cggcggcgca
     2461 gcgatagggg tgggccgggc cgaggagccg gcagtcagtg ccgggggcga ggccggcgcg
     2521 gagggcagcg aggcaggcgg ccgggccacc tctcgcgacg gcccggccga ggccagctgc
     2581 ccagaggttg ccaccccggc cggtgacggc gcggcgaccg gctcgccggg ctgggcgagg
     2641 acctgctgcc caatccccag caggtccaca tggccctcgg gcgaaacctc actgccgccg
     2701 cccggctgca gatcgacggt ccgaggcaga tcgggcccag gttcgctc
//
LOCUS       DAOPQX010000431         2699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_41038_length_2699_cov_4.213691, whole genome shotgun sequence.
ACCESSION   DAOPQX010000431 DAOPQX010000000
VERSION     DAOPQX010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41038_length_2699_cov_4.213691"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>2699
                     /locus_tag="PKK95_16210"
     CDS             59..>2699
                     /locus_tag="PKK95_16210"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19814.1"
                     /translation="MGRPAQAAASAAPVPVLASRRFKSAHTVSPHAQALSNGRYTTML
                     THAGGGWSAWRELAVTRRREDRTSDFGAHFIYFRDPWSGQVWSPTYLPTCVEPDSYEV
                     TFALDKATYRRRDGDLESQMQVAVSPEDDVEVRRVTITNRGDRPRELEVTSYAEIVLG
                     RPEDDLAHPAFGKLFIETEYDAQSAGLLFSRRPRASDDPPAWAFHVLGLDGRFGGAVE
                     WETDRARFLGRGCTIARPAAIDGPLSGTTGAVLDPVASLRERVRLAPGAFVRLTFATG
                     AAPTRAAALTLVQKYRDASAASRVFSMAFTHAHITLQHLGLTDSQAMLFDRLGSRVFG
                     ADATCISPADLAGNTLGQPALWAYGISGDLPIVLLRVIDPAGVALARQLLHAQEYWRV
                     KSLRADLVILNEQPVDYLDDLQGSLAALVREPRFAGWHDKPGGVFLLRTDGMAEADRR
                     LLAAVARVVLRSDLGELEPQLDRPAPWLYPAQPPAETLAPGRPAPAAEPVPVPPLVME
                     NGIGGFTDGGREYVIVLQDGRDTPLPWSNVLANPRFGTIASESGAAFTWAINSRENRL
                     TPFANDPVSDPTAEAIYIRDDEAHVAWGATPAPLPRVEGGRWVVRHRAGATRYQHAIE
                     GLEQELEVFVPPDDPVKVSVLTIRNTSEATRRLSVFGYVEWCLGPPRQGERRFVITEV
                     DEDAGAIFARNPYNSEFAGRVAFLRATAAPASFTCDRGDFIGRQRTASNPAGLGRERL
                     ANRSGAGLDPCGALQVPIEIPPGETRRLAFVLGQGGNAAEAAELAARYASLEAVEAAA
                     ERCNRFWDDTLGAIQVKTPDDSFDLIVNRWLLYQSLSCRMWAKSGPYQPGGAWGFRDQ
                     LQDALALLYTRPDLCRAHLLEAAS"
BASE COUNT          357 a         1020 c          959 g          363 t
ORIGIN      
        1 caggacagtg gcgggttcgg gatcggactc gtgggtcagc cactcataga cctgtgtgat
       61 gggtcggccg gcgcaggccg cggcctccgc cgccccggtc ccggtgctgg cgtcgcgccg
      121 cttcaagtcg gcgcacacgg tgagccccca cgcgcaggcc ctgtcgaacg ggcgctacac
      181 gacgatgctg acccacgccg gcggcggctg gagcgcgtgg cgcgagctgg ccgtgaccag
      241 gcggcgcgag gaccgcacgt cggacttcgg cgcccacttc atctacttcc gcgacccgtg
      301 gtcggggcag gtctggtcgc ccacctacct gcccacctgc gtggagcccg acagctacga
      361 ggtgaccttc gcgctcgaca aggcgaccta ccgccggcgc gacggcgacc tcgagagcca
      421 gatgcaggtg gccgtctcgc ccgaagacga cgtggaggtg cggcgcgtga ccatcaccaa
      481 ccgcggcgac cggccgcggg agctggaggt caccagctac gcggagatcg tgctcgggcg
      541 cccggaggac gacctggcgc acccggcgtt cgggaagctg ttcatcgaga cggagtacga
      601 cgcgcagagc gcgggcctgc tcttcagccg tcgcccccgc gcctcggacg atccgcccgc
      661 ttgggccttc cacgtgctcg ggctggacgg ccgcttcggc ggggccgtcg agtgggagac
      721 cgaccgcgcc cggttcctgg gccgcggctg cacgatcgcc cggccagccg ccatcgacgg
      781 tcccctctcg ggcaccaccg gcgccgtgct cgacccggtg gcctcgctgc gcgagcgcgt
      841 ccgcctcgcc cccggcgcgt tcgtcaggct caccttcgcc accggcgccg cgccgacgcg
      901 tgccgccgcg ctgaccctcg tccagaagta ccgcgacgcg agcgccgcct cgcgtgtctt
      961 ctcgatggcg ttcacgcacg cgcacatcac gctgcagcac ctcgggctga ccgacagcca
     1021 ggcgatgctc ttcgaccgcc tcgggtcgcg ggtcttcggc gccgacgcca cgtgcatcag
     1081 ccccgccgac ctggcgggca acacgctcgg ccagccggcg ctctgggcct acggcatctc
     1141 gggcgacctc ccgatcgtgc tgctgcgggt catcgacccg gccggggtgg ccctggcgcg
     1201 gcagctgctg cacgcgcagg agtactggcg cgtcaagagc ctgcgcgccg acctcgtcat
     1261 cctcaacgag cagccggtgg actacctcga cgacctgcag ggatcgctgg ccgcgctggt
     1321 gcgcgagccg cgcttcgcgg ggtggcacga caagccggga ggcgtcttcc tgctgcgcac
     1381 cgacgggatg gcggaggccg accgccgcct gctcgccgcc gtggcccgcg tcgtgctccg
     1441 cagcgacctc ggcgagctgg agccgcagct ggatcgcccg gcgccctggc tctacccggc
     1501 gcagccgcct gcggaaaccc tcgcgcccgg acgccccgct ccggccgccg agccggtgcc
     1561 cgtgccgccg ctcgtgatgg agaacggcat cggcgggttc accgacggcg gtcgagagta
     1621 cgtcatcgtg ctccaggacg ggcgtgacac tcccctcccc tggtcgaacg tgctcgccaa
     1681 tccccggttc ggaacgatcg ccagcgaatc gggcgcggcc ttcacgtggg cgatcaacag
     1741 ccgcgagaac cgcctgaccc cgttcgccaa cgacccggta tcggacccga ccgccgaggc
     1801 gatctacatc cgtgacgacg aagcgcacgt tgcctggggc gcgacgccgg cgccgctgcc
     1861 gcgcgtggaa gggggccgct gggtcgtgcg gcaccgcgcg ggggcgaccc gctaccagca
     1921 cgcgatcgaa ggcctcgagc aggagctcga ggtcttcgtg ccgcccgacg acccggtgaa
     1981 ggtgtccgtg ctgacgatca ggaacacctc cgaagcgacg cgccggctga gcgtgttcgg
     2041 gtacgtcgag tggtgccttg gaccgccgcg ccagggcgag cgccgcttcg tgatcaccga
     2101 ggtggacgag gatgccggcg cgatcttcgc gcgcaacccg tacaacagcg agttcgccgg
     2161 ccgcgtcgcc ttcctgcgcg ccaccgcggc gcccgcttcg ttcacgtgcg atcgcggcga
     2221 cttcatcggc cgccagcgca ccgcgtcgaa ccccgcgggg ctcggccgcg agcgcctcgc
     2281 caaccggagc ggcgccggcc tcgacccctg cggcgccctg caggtgccga tcgagatccc
     2341 gcccggcgag acgcggcggc tggcgttcgt gctcggccag gggggcaacg ccgccgaggc
     2401 cgcggagctg gccgcccgct acgcgtcgct cgaggcggtc gaggcggccg ccgagcgctg
     2461 caaccggttc tgggacgaca cgcttggcgc gatccaggtg aagacgccag acgactcgtt
     2521 cgacctgatc gtgaaccgct ggctgctgta ccagtcgctg agctgccgca tgtgggcgaa
     2581 gagcggtccc tatcagcccg ggggcgcctg gggattccgc gaccagctgc aggacgcgct
     2641 ggcgctgctt tacacgcggc ccgacctctg ccgcgcgcac ctgctcgagg cggcgtcgc
//
LOCUS       DAOPQX010000432         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_41648_length_2669_cov_4.489288, whole genome shotgun sequence.
ACCESSION   DAOPQX010000432 DAOPQX010000000
VERSION     DAOPQX010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41648_length_2669_cov_4.489288"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKK95_16215"
     CDS             <1..601
                     /locus_tag="PKK95_16215"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOC19815.1"
                     /translation="PIFLAVAFLAGMRLRLLGILLILAVVTAPVAWRYALKDYQRSRI
                     ATFVNPEQDPRGAGYQQIQARISVGSGGIWGKGFLKGTQGQLRFLPVAWNDFVFSVLA
                     EEQGFVGVIVALGLYLFVIVRSLEAARLSKDRLGAYLVLGILATFAFQVIYNITMSAG
                     LAPVKGLTLPLMSSGGSSIIATLAGFGLILNVRMRRFTN"
     gene            695..2215
                     /locus_tag="PKK95_16220"
     CDS             695..2215
                     /locus_tag="PKK95_16220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOC19816.1"
                     /translation="MNKEMIVSSNGHQTMVAILEDDQVSEVFIERERQRGIVGNVYKG
                     RVSKVLPGMQSAFVDVGLERDGFLYVSDVVDTIEEFERLESGDDENGDEEPERRGGAQ
                     AKIEELIREGQDIIVQVAKEPLGTKGARLTSHVTMAGRFLVFMPTVDHVGVSRKIESR
                     EERARLRGIVREFREQHGFTGGVIIRTAAAGRPPEDLTADLEYFQRIWGEVRQRADSV
                     RAPAVIYRELGLVSKLLRDLLSSDYSVIRLDDTQDYQQAKELVQRLMPQVAGRVKLYQ
                     KPFPIFEEYGVQQEIEKALRSKVWLKSGGYIVVNQTEALVAIDVNTGRYVGKKNGRLE
                     DTIVKTNLEAVREIVRQIRLRDLGGIIVLDFIDMEEKKNRQKVLQAMEQELKKDRSPS
                     KAVAVSDFGLIIITRKRVKQSLERTLTEPCPYCSGSGVIKSTATICYEILDEVRKIGP
                     ELDGAGVVLRVNPEIARALQEEESGVLRELRETIRKEVTLRPDHLLHHEQFDVMAR"
     gene            complement(2278..>2669)
                     /locus_tag="PKK95_16225"
     CDS             complement(2278..>2669)
                     /locus_tag="PKK95_16225"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOC19817.1"
                     /translation="RDAQVRGLSAETVSGELRLANVSGDRVGAKSVSGGVDFSGPLAQ
                     GGRYEMKSHSGDVRVAVANDAGFELEATSFSGNVRSDLPVTIGGPGREAVRTGGRGRQ
                     AMRGVHGDGSAFLSLTTFSGSIVVTKR"
BASE COUNT          438 a          884 c          942 g          405 t
ORIGIN      
        1 gccgatcttc ctggcggttg ccttcctcgc gggcatgcgc ttgcggctgc tcggcatcct
       61 gctgatcctc gcggtggtga ccgcgccggt ggcgtggcgc tatgcgctga aggactacca
      121 gcggagccgg atcgcgacct tcgtcaaccc cgagcaggac ccgcgcgggg cgggctacca
      181 gcagatccag gcccggatca gcgtgggctc gggcgggatt tggggcaagg ggttcctcaa
      241 gggcacccag gggcagctgc gcttcctgcc ggtggcctgg aacgatttcg tcttctcggt
      301 gctggccgag gagcaggggt tcgtcggcgt catcgtggcg ctgggactgt atctgttcgt
      361 gatcgtcagg tcgctggagg cggcgcggct gtccaaggat cggctggggg cctacctggt
      421 tctcgggatc ctggccacct tcgccttcca ggtgatctac aacattacga tgtcggccgg
      481 gctggcgccg gtgaaggggc tcaccttgcc gctgatgagc tccggagggt cgtcgatcat
      541 cgcgaccctc gccggcttcg gcctgatcct gaacgtcagg atgcgccgat tcacaaatta
      601 ggctcagggc tcacggctca cggctcaggg cggcgtttcg gatcgcgccg ccagccgctg
      661 gccgcaggcc gctggcccaa gagatcgccc gtccatgaac aaggagatga tcgtctcctc
      721 gaacggccac cagaccatgg tggccatcct cgaggacgac caggtcagcg aagtcttcat
      781 cgagcgcgag cgacagcgcg ggattgtcgg caacgtctac aaggggcgcg tctcgaaggt
      841 gctgcccggg atgcagtcgg ccttcgtgga cgtcggcctc gagcgcgacg gcttcctgta
      901 cgtcagcgac gtggtcgaca ccatcgagga gttcgagcgg ctcgagtcgg gcgacgacga
      961 gaacggcgac gaggagcccg agcgccgcgg cggggcgcag gccaagatcg aggagctgat
     1021 ccgcgagggc caggacatca tcgtgcaggt ggccaaggag cccctgggca ccaagggcgc
     1081 ccggctcacc tcccacgtca cgatggccgg gcgcttcctc gtcttcatgc ccaccgtgga
     1141 ccacgtcggg gtctcgcgca agatcgagtc gcgcgaggag cgggcgcgcc tgcgcggcat
     1201 cgtgcgcgag ttccgcgagc agcacggctt caccggcggc gtcatcatcc gcaccgccgc
     1261 cgccggccgc ccccccgagg acctcacggc cgacctcgag tacttccagc gcatctgggg
     1321 agaggtgcgc cagcgcgccg actcggtgcg ggccccggcg gtcatctacc gcgagctggg
     1381 gctggtgagc aagctgctcc gcgacctgct gagcagcgac tactcggtca tccggctcga
     1441 cgacacccag gactaccagc aggcgaagga gctggtgcag cggctcatgc cgcaagtggc
     1501 cggccgcgtg aagctctacc agaagccgtt cccgatcttc gaggagtacg gcgtccagca
     1561 ggagatcgag aaggcgctgc ggagcaaggt ctggctcaag tcgggcggct acatcgtcgt
     1621 caaccagacc gaggcgctgg tggccatcga cgtgaacacg gggcggtacg tcgggaagaa
     1681 gaacggccgc ctcgaggaca ccatcgtcaa gacgaacctc gaggcggtgc gggagatcgt
     1741 gcggcagatc cggctgcgcg acctgggcgg catcatcgtc ctcgacttca tcgacatgga
     1801 ggagaagaag aaccgccaga aggtgctgca ggcgatggag caggagctga agaaggaccg
     1861 ctcgccctcc aaggcggtcg cggtgtcgga cttcggcctg atcatcatca cccgcaagcg
     1921 cgtgaagcag agcctcgagc ggacgctcac cgagccgtgc ccgtactgtt cgggcagcgg
     1981 cgtcatcaag tccaccgcca ccatctgcta cgagatcctc gacgaggttc ggaagatcgg
     2041 gcccgagctg gacggggccg gcgtggtgct gcgcgtcaac cccgagatcg cgagggcgct
     2101 gcaggaagag gaaagcggcg tgctgcgcga gctgcgcgag acgatccgga aggaggtgac
     2161 cctgcgcccg gatcacctcc tccaccacga gcagttcgac gtgatggcgc ggtaacgcga
     2221 aggcctggct tgccgacggg ccgccgtcct ccatgacggg tggccgcccg ttgtgaccta
     2281 tctcttcgtt acgacgatcg agcccgagaa ggtggtcagc gagaggaagg cgctgccgtc
     2341 gccgtggacg ccgcgcatcg cctggcgccc gcggccgccg gtccgaacgg cctcgcggcc
     2401 cgggccgccg atggtgacgg gcaggtcgga gcggacgttc ccgctgaagc tggtggcctc
     2461 gagctcgaac cccgcgtcgt tggcaaccgc cacccgaacg tctcccgagt gcgacttcat
     2521 ctcgtagcgt ccgccctggg ccagcggccc cgagaaatcg acgccgccgc tcaccgactt
     2581 ggcgccgacg cggtcgccgc tgacgttggc gagccgcagc tcgccgctga ccgtctcggc
     2641 cgagaggccc cgcacctgcg cgtcgcgca
//
LOCUS       DAOPQX010000433         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_42662_length_2621_cov_3.854248, whole genome shotgun sequence.
ACCESSION   DAOPQX010000433 DAOPQX010000000
VERSION     DAOPQX010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42662_length_2621_cov_3.854248"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKK95_16230"
     CDS             complement(<1..272)
                     /locus_tag="PKK95_16230"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HOC19818.1"
                     /translation="MFPDAYKYTSDHEWFDPATGRMGITDYAQKQLGDIVFLELPEVG
                     RHVEKGDQVGTVESVKAVSEIFAPLAGTVKEVNAALTEKPEGIKRTR"
     gene            complement(276..1388)
                     /gene="gcvT"
                     /locus_tag="PKK95_16235"
     CDS             complement(276..1388)
                     /gene="gcvT"
                     /locus_tag="PKK95_16235"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOC19819.1"
                     /translation="MSQSTTLRTTPLAAWHRANGGHMVPFAGWEMPVEYSGLGEEHLA
                     VRTRAGLFDVSHMGQVELAGKDALAAVQQMTSNDASKLKVGQAHYSALMTPAGTFVDD
                     LLVYRFANEHFLFVINASNAEKDVNWIVEAARPFGDVAVVDTSSRYALIALQGPAARE
                     VLQTQTDVDLATIKYYWFAPGEVAGVRVTISRTGYTGESGYEVFTPPQHAVKVWEALL
                     NAGRGAGVVPCGLGARDTLRLEAAMRLYGNDIDDTTTVLEADLEWIVGWNKGDFTGRA
                     ALEAQKANGLARRLVGFEMVDRAIARHGYDALVDGRKAGTVTSGTQTPFLKKAIGMAY
                     LPVDRTEPGTEFEVDVRGRRARARVVPMPFYKRPKG"
     gene            complement(1534..>2621)
                     /locus_tag="PKK95_16240"
     CDS             complement(1534..>2621)
                     /locus_tag="PKK95_16240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOC19820.1"
                     /translation="GGIQQEAAMIIANGPPSAAVPGGQDGLGWVPGGLIGASLAMRRL
                     REHIARVATTSFTVLIEGESGTGKELVARQIHALSPRCHGPFVAVNCAAIVDTLLEAE
                     LFGIEDRTATGVRGRRGKFEHAHGGTLFLDEVSDLAPAAQAKLLRVIQDPTVERVGGH
                     ASRRVDARIVVATNRALRGLAARGVFRADLYYRLSGLEVHVPPLRSRREDIPELVQHF
                     LSRDRGGRNLSLTKAAEEALRAYEWPGNVRELERLVEGAVALARSDRITLEDLPAVVR
                     GDYGEVLLPSLTREETMRAWGSRYARLTLERCAGNKRLACRILDISYHTLQAYLRYGS
                     ETLAPRLPPGNGSNGGCRTAGEHAIEP"
BASE COUNT          369 a          951 c          893 g          408 t
ORIGIN      
        1 cgcgtcctct tgatcccttc gggcttctcg gtgagggcgg cgttgacctc cttcaccgtg
       61 ccggccagcg gcgcgaagat ctccgacacc gccttgaccg actccaccgt tcccacctgg
      121 tcgcccttct cgacgtgcct gcccacctcg ggcagctcga gaaagacgat gtcaccgagc
      181 tgcttctggg cgtagtcggt gatgcccatg cggccggtcg ccgggtcgaa ccactcgtga
      241 tcgctcgtgt acttgtaggc gtcgggaaac atcgctcacc ccttcggtcg tttgtagaac
      301 ggcatcggca cgacgcgcgc gcgcgcccgc cggccccgga catccacctc gaactcggtc
      361 cccggctcgg tgcggtcgac cggcaggtag gccatgccga tcgccttctt gaggaacggc
      421 gtctgcgtcc cgctcgtcac ggtgccagcc ttccggccgt cgacgagcgc gtcgtagccg
      481 tgccgggcga tcgcgcggtc caccatctcg aagcccacga gccgccgcgc caggccgttg
      541 gccttctgcg cctccagcgc ggcccgcccg gtgaagtcgc ccttgttcca gccgacgatc
      601 cactcgaggt cggcctcgag caccgtcgtc gtgtcgtcga tgtcgttgcc gtagaggcgc
      661 atcgccgcct cgaggcgcag cgtgtcgcgg gcgccgagcc cgcacgggac gacgccggcg
      721 ccgcggccgg cgttcaggag cgcctcccag accttcacgg cgtgctgggg cggcgtgaag
      781 acctcgtagc cgctctcccc cgtgtacccc gtgcgcgaga tcgtcacccg aaccccggcc
      841 acctcgcccg gcgcgaacca gtagtacttg atggtcgcca ggtccacgtc ggtctgcgtc
      901 tgcagcacct cgcgggccgc cggcccctgc agggcgatca gcgcgtaccg agagctggtg
      961 tcgaccaccg cgacgtcgcc gaacggcctg gcggcctcga cgatccagtt gacgtccttc
     1021 tcggcgttcg aggcgttgat cacgaacagg aagtgctcgt tcgcgaagcg gtacacgagc
     1081 aggtcgtcga cgaaggtgcc ggccggcgtc atcagcgcgg agtagtgcgc ctggccgacc
     1141 ttcagcttcg aggcgtcgtt gctcgtcatc tgctggacgg cagcaagggc gtccttaccg
     1201 gccagctcga cctggcccat gtggctgacg tcgaacaggc cggcgcgcgt ccggacggca
     1261 aggtgctcct cgccgaggcc gctgtattcg accggcattt cccacccggc gaacggaacc
     1321 atgtgcccgc cgttggcgcg gtgccaggcc gcgagcggag tggtgcgaag cgttgtggat
     1381 tggctcatcg gataggcctg cgggaagggt gaacgaacag acaggtaaca ctactacagg
     1441 gatggctcgg ggctcaaggc ttgcgcctcg cgccaagaag gcaggcgacg ccccttgggg
     1501 agggggtcga gccgcagcgt tacgggcggc ggctcatggc tcgatcgcgt gctcgccggc
     1561 ggtgcggcac cctccattcg atccgttgcc cggggggagg cgcggcgcga gcgtttcgct
     1621 cccgtagcgc aggtacgcct gaagggtgtg gtagctgatg tccaggatgc ggcacgcgag
     1681 acgcttgttg ccggcgcacc gctcgagcgt gagccgcgcg tagcggctgc cccacgcccg
     1741 catcgtctcc tccctggtga gcgagggcag gagcacctcg ccgtagtcgc cgcgcacgac
     1801 cgcgggaaga tcctcgagcg tgatccggtc cgagcgcgcg agcgccaccg ccccctcgac
     1861 cagccgctcc agctcgcgca cgttgcccgg ccactcgtac gcgcgcagag cctcttcggc
     1921 cgccttggtg agcgagaggt tgcggccgcc gcggtcgcgc gagaggaagt gttgcaccag
     1981 ctcggggatg tcctcgcgcc ggctgcgaag cgggggcacg tgcacctcga ggcccgacag
     2041 ccggtagtag aggtcggccc ggaagacgcc tcgcgccgcg agaccgcgca gggcccggtt
     2101 ggtcgccacc acgatgcggg catccacccg ccggctggcg tgcccgccca cgcgctcgac
     2161 cgtcgggtcc tggatgactc gcagcagctt cgcctgcgcg gccggcgcca ggtcggaaac
     2221 ctcgtcgagg aacaacgtgc cgccgtgcgc gtgctcgaac ttcccccgcc gtccgcgcac
     2281 gccggtcgcc gtgcggtctt cgatgccgaa cagctcggcc tcgagcagcg tgtccacgat
     2341 ggccgcgcag ttcaccgcga cgaacggacc gtggcagcgc gggctcaagg cgtgaatctg
     2401 gcgcgccacc agctccttgc cggttcccga ctcgccttcg atgagcacgg tgaaggaggt
     2461 cgtagcaacc cgggcgatgt gctcccgcag ccgacgcatc gccaggcttg ccccgatcag
     2521 gccgcccggc acccacccga gcccatcctg cccacccggc acggctgccg aagggggccc
     2581 gttcgcgatg atcatggccg cctcctgctg aatgccccca g
//
LOCUS       DAOPQX010000434         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_43574_length_2580_cov_4.289109, whole genome shotgun sequence.
ACCESSION   DAOPQX010000434 DAOPQX010000000
VERSION     DAOPQX010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43574_length_2580_cov_4.289109"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PKK95_16245"
     CDS             complement(<1..877)
                     /locus_tag="PKK95_16245"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOC19821.1"
                     /translation="MPYRRLFVAALTLVALCPSLAVAFQGRIVFQGTGQPAAGAEVSV
                     LGRTGTARTDEDGLFAWTPDPAPPFEVLVILPGGRHMRPVLVQRLPKEGPVIIEVAPL
                     VSETVSVTAGAAPWIETTPGSGMTLLSSSDLAIRQPVNLAQLLENVAGASTVSEGHAA
                     VPAIRGLARGRTLILIDGARVTSERRVGPSATFLDPFVLEGVEIARGPGSVAYGSDAF
                     GGVIHARTRRVDPTSPFGFRFQGTLGAGVPEMRGGVELSKGLGKGGFLLQAHARRFDD
                     YRSPEGEVFNSGAKDQ"
     gene            1081..1569
                     /locus_tag="PKK95_16250"
     CDS             1081..1569
                     /locus_tag="PKK95_16250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOC19822.1"
                     /translation="MSNPTLPREPLSAEVAGVPGVVCIDHLAIAVKAGELDAQAAAYR
                     LLGFTEIHREEVLGGDQVREVLLRIGDGPNLVQLLEPLTPDSPVQKLIDRNGGRGGFA
                     HVAFRVTDIRQAFDRMKVDGFNIIDKAPRKGSRGTTVFFVHPKSRETAPFGFLIEMVQ
                     EG"
     gene            1629..>2580
                     /locus_tag="PKK95_16255"
     CDS             1629..>2580
                     /locus_tag="PKK95_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOC19823.1"
                     /translation="MPDETQQQKRLSLIDEFPPVPTAAWEEAIARDLKGADYEKRLVW
                     KTDEGIAIRPYYRAEHVALREGIFARGAWEIASPGAEPAADVWAVEAHEAGATAVQEL
                     AFALAAAAELPAGRETTRVAFAIGSNHFMEIAKLRAFRLLWEKLDGGGGPGPLRIHAR
                     TAGENKTLYDPHVNLLRVVTEALSAVLGGCDSLTITPCRFDAHLADNVHRILSEESHL
                     DKVSDPGAGSYYVEWLTDALAGAAWTLFEQIQAAGGYTRYRASGELDAALAASRAAKE
                     KAVASRRRVLVGTNSYPNLRERVLESADEMPEGWRLASAFE"
BASE COUNT          416 a          918 c          915 g          331 t
ORIGIN      
        1 cctggtcctt cgcgcccgag ttgaacacct ctccctcggg gctgcggtaa tcgtcaaagc
       61 gccgggcgtg cgcctgcagc aggaacccgc ccttgccgag ccccttcgaa agctcgacgc
      121 cgccgcgcat ctcgggaacg ccggccccga gcgtgccctg gaaccggaac ccgaacggcg
      181 aggtcggatc gacgcgtcgc gtgcgcgcgt ggatgacgcc gccgaaggcg tccgacccgt
      241 acgcgaccga cccggggccg cgcgcgatct ccaccccctc gagcacgaac ggatcgagga
      301 aggtggcgct cggtccgacc cgccgctccg aggtcacgcg ggcgccgtcg atcaggatca
      361 gcgtccggcc gcgggccagg ccgcgaatgg cgggcaccgc ggcgtgtccc tcggaaaccg
      421 tcgaggcgcc cgcgacgttc tcgagcagct gggcgaggtt gaccggctgg cggatggcca
      481 ggtcggatga ggagagcagc gtcatcccgc tgccgggggt cgtctcgatc cacggcgccg
      541 ccccggcggt cacgctgacc gtctccgaca ccagcggcgc cacctcgatg atgacggggc
      601 cttccttggg caggcgctgg acgagcaccg gacgcatgtg gcgcccgccg gggaggatga
      661 cgagcacctc gaagggcggc gccgggtcgg gcgtccaggc gaagagcccg tcctcgtcgg
      721 tgcgggcggt tcccgtccgc cccagcaccg acacctcggc gcccgcggcc ggctggccgg
      781 taccctggaa gacgatacgg ccctggaaag caaccgccag cgaggggcac agcgcaacga
      841 gggtcagggc ggcgacgaag aggcggcggt acggcatgga tacccgccac tataccagcc
      901 cgtggtgcca ctctacgcgg gctgccacgg ccggccgcgg ccctcgggac cctaagacgg
      961 cggcttggcc gccggacggc ccgcgcagta gaatttcctg ttcgggatgg tgcccgccgc
     1021 gccgtgatgg tcccccagcg ccaccttccg cccgcacccc cgtcacgaag gaggacgtcc
     1081 ttgtcgaacc cgaccctgcc ccgggaaccg ctgagcgccg aggtcgctgg cgtccccggc
     1141 gttgtctgca tcgaccacct cgccatcgcc gtcaaggccg gcgagctcga cgcacaggcc
     1201 gctgcctacc gcctgctcgg cttcaccgag atccaccgcg aggaagtgct cggcggagac
     1261 caggtccgcg aggtgctgct gcggatcggc gacggaccga acctggtgca gctgctcgag
     1321 ccgctgacgc ccgactcgcc ggtgcagaag ctgatcgacc gcaacggcgg gcggggcgga
     1381 ttcgcccacg tcgccttccg cgtcaccgac atccggcagg cgttcgaccg gatgaaggtc
     1441 gacgggttca acatcatcga caaagctccc cgaaaggggt cgcgaggcac caccgtgttc
     1501 ttcgtccacc ccaagtcgcg cgagaccgcc ccgttcggct tcctgatcga gatggtgcag
     1561 gaagggtagg accaggggag cggagcactg aaaacggaga ccggaaaacg gggaaagcag
     1621 aagggatcat gcccgacgag acgcagcagc agaagcggtt gtccttgatc gacgagttcc
     1681 cgccagtgcc caccgccgcg tgggaggaag cgattgcacg cgatctgaag ggggccgact
     1741 acgagaagcg gctggtctgg aagaccgacg aggggatcgc gattcgcccg tactaccgcg
     1801 cagagcacgt cgcgctgcgc gaggggattt tcgcccgcgg agcctgggag atcgcatcgc
     1861 cgggagccga gccggccgcc gacgtgtggg cggtcgaggc ccacgaggcg ggagccaccg
     1921 cggtccagga gctggccttt gcactggcgg cagcggccga actgccggcc ggccgcgaga
     1981 cgacgagggt ggccttcgcc atcggctcga accacttcat ggagatcgcg aagctgcgcg
     2041 ccttccgcct cctgtgggag aagctggacg gcggaggcgg ccccggccct ctgcgcatcc
     2101 acgcccggac cgccggcgag aacaagaccc tctacgatcc gcacgtcaac ctgcttcgag
     2161 tcgtgaccga ggcgctctcg gcggtgctcg ggggctgcga ctcgctcacg atcacgccct
     2221 gccgcttcga cgcccacctg gccgacaacg tccatcgcat cctcagcgag gagagccacc
     2281 tcgacaaggt gtccgacccg ggcgccggct cgtactacgt cgagtggctc accgacgcgc
     2341 tggccggcgc ggcgtggacg ctcttcgagc agatccaggc cgcggggggg tacacccgtt
     2401 accgggcctc gggcgagctg gacgcggcgc tcgccgcttc gcgggcggcg aaggaaaagg
     2461 cggtcgcctc gcgccgccgc gtgctcgtgg gcaccaacag ctaccccaac ctgcgagagc
     2521 gcgtgctcga gtcggccgac gagatgcccg agggttggcg cctggcctcc gcgttcgagg
//
LOCUS       DAOPQX010000435         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_43580_length_2580_cov_3.573069, whole genome shotgun sequence.
ACCESSION   DAOPQX010000435 DAOPQX010000000
VERSION     DAOPQX010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43580_length_2580_cov_3.573069"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PKK95_16260"
     CDS             <1..962
                     /locus_tag="PKK95_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19824.1"
                     /translation="VFFLPSHLVVALWAGCGAAAVIRTLPSPSVLRYAVPVVLLAFPA
                     WRAWDAWPALDRSSDTAPRQFFDRLLSDLAPERESVVAALGWQQHNGIDYYGRHTRPD
                     LAIGHLRPVLLHFPFLVSQNAALGRDLVLTDEAALLVRETYGPLFAIERDARLPTPSL
                     VEQLRALPAGSPYALTMLKPYREGGIEPGEVAAAVAILAGRPTSVPSGDYLAVAGRTG
                     SEPDLVRAANRPFRVKVPIAGRILDVRMESWLSTDTIRRAGFGHVVEGRRHLLAIDRG
                     LSVVALGPDGEVLLGTWQGGMLTPQGRWVIRAAQDSGPKGPGS"
     gene            1005..1667
                     /locus_tag="PKK95_16265"
     CDS             1005..1667
                     /locus_tag="PKK95_16265"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOC19825.1"
                     /translation="MLSAIVIALVLPLQAAQPETAPQPAEDLARRLQAKYEQVRDFTA
                     DFEHAYTGGVLRRKAVERGRVQVKKPGKMRWIYTSPEKKEFVSDGMKLYSYIPEDQQV
                     MVSSVPPADEASSPALFLAGKGDVLRDFTPSFAEVPNLPASQVALKLMPKREQAEYDS
                     LILVLDRQSLRIERLITTDAQGGTSSFTFTNIRENVGLPDKTFTFTIPRGVDVITDAG
                     KK"
     gene            1639..>2580
                     /locus_tag="PKK95_16270"
     CDS             1639..>2580
                     /locus_tag="PKK95_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19826.1"
                     /translation="MSSPMQARSSLGAACVTLALAALLAGCASSQALRRAREAERRQD
                     YDRAVAEYTRLVKQDPDNAEARLALQRTKLRAADAHFNRGRRFAAAGRLDEALIEFQI
                     ASELNPASADIDTALQETRNQARARIAVASEGRTALESLIERTRQMPSPGQELPAELK
                     LPASLVFRDASSRDVFTAVARFADINVVFDPAFREAAVTIDLRDATLEQALGAVTAST
                     RNFYRVTAPRTITIIPDTPAKRREYEDEVVRTFYLSNADLKETIDLLRMVVDLRRLAP
                     ITATNAISIKDTPERIEAAARLIGAIDKARPEVVNDVE"
BASE COUNT          400 a          938 c          868 g          374 t
ORIGIN      
        1 acgtgttctt cctgccgtcg cacctcgtcg tggcgctgtg ggcggggtgc ggggcggccg
       61 ccgtcatccg cacgctccct tctccgtccg ttctccgtta cgcggtcccc gtcgtgctgc
      121 tcgcctttcc agcctggcga gcgtgggacg cctggcccgc cctcgatcgc agcagcgaca
      181 ccgccccccg ccagttcttc gatcgcctgc tgtcggacct cgccccggaa cgcgagtcgg
      241 tcgttgccgc ccttggctgg cagcagcaca acggcatcga ctactacggg cgccacacgc
      301 gccccgacct ggcgatcggg cacctccgcc ccgtgctcct ccacttcccc ttcctggttt
      361 cgcagaacgc cgcgctcggc cgcgacctcg tgctcaccga cgaggcggcg ctgctggtgc
      421 gcgagacgta cggcccgctg ttcgccatcg agcgcgacgc gcggctcccg accccgtcgc
      481 tcgtcgagca gcttcgcgcg cttcccgccg gcagccctta cgcgctgacg atgctcaagc
      541 cgtacaggga gggaggcatc gagccagggg aggtggcggc ggcagttgcc atacttgccg
      601 gccgcccgac ctcggtccct tccggcgact acctcgccgt cgcggggcgg acgggatccg
      661 aacccgacct ggtacgtgcg gcgaaccggc cgttccgggt gaaggtgccc atcgccggcc
      721 gaatactgga tgtgcgaatg gaatcgtggc tttcgaccga cacgatccgc agggcgggct
      781 tcgggcacgt ggtcgagggg cggcgccacc tgctcgcgat cgacaggggc ctctcggtcg
      841 tggcgctcgg accagacgga gaggtactgc tcggaacctg gcagggcgga atgctgacgc
      901 ctcagggaag gtgggtgata agggcggctc aggactcagg gcccaagggg cctgggtcct
      961 gaggcggccc agggcgcggg gctgggcgta caatggttcc atccatgttg tctgcaatcg
     1021 tcattgcgct cgtgctccct ctccaagcgg cccagcccga aacggctccc cagccggccg
     1081 aggatctcgc ccgccggctg caggcgaagt acgagcaggt gcgcgatttc acggccgact
     1141 tcgagcacgc ctacacggga ggcgtcctgc gccgcaaggc ggtcgagcgc ggccgcgtgc
     1201 aggtgaagaa gcccggcaag atgcgctgga tctacacctc ccccgagaag aaggagttcg
     1261 tctcggacgg gatgaagctc tactcgtaca ttcccgagga ccagcaggtg atggtcagca
     1321 gcgtcccccc tgccgacgag gcgagcagcc ccgcgctgtt cctcgccggc aagggcgacg
     1381 tgctccgaga cttcaccccc tcgttcgccg aggtgcccaa cctcccggcc agccaggtgg
     1441 cgctgaagct gatgcccaag cgcgagcagg ccgagtacga ctcgctgata ctcgtgctcg
     1501 accgccagag cctccggatc gagcggctga tcaccaccga cgcgcagggg ggcacctcgt
     1561 cgttcacctt cacgaatatc cgcgagaacg tggggttgcc agacaagacg ttcaccttta
     1621 caatcccgcg cggggtggat gtcatcaccg atgcaggcaa gaagtagcct cggcgccgcc
     1681 tgcgtgacgc tcgccctggc ggcgttgctg gcgggctgtg ccagctcgca ggcgctgcgg
     1741 agggcccgcg aggccgagcg gcgccaggac tacgatcgcg ccgtcgccga gtacacccgc
     1801 ctggtcaagc aggaccccga caacgccgag gcccggctcg ccctgcagcg caccaagctg
     1861 cgcgcggccg acgcccactt caaccggggc cggcgcttcg cggccgccgg ccgcctggac
     1921 gaggcgctga tcgaattcca gatcgcgtcg gagctgaacc cggcgagcgc cgacatcgac
     1981 acggcgctgc aggagacgcg caaccaggcg cgggcccgga tcgcggtcgc gagcgagggg
     2041 cggacggccc tcgagtcgct catcgagcgg acgcggcaga tgccctcgcc cggccaggag
     2101 ctgccggcgg agctgaagct tcccgcctcg ctcgtgttcc gcgacgccag cagccgcgac
     2161 gtcttcaccg ccgtcgcccg cttcgccgac atcaacgtcg tcttcgaccc ggccttccgc
     2221 gaggcggcgg tcaccatcga cctgcgcgac gccacgctcg agcaggcgct cggggcggtg
     2281 acggccagca cgcgcaactt ctaccgggtc accgcgccgc ggacgatcac catcatcccc
     2341 gacacacccg ccaagcggcg ggagtacgag gacgaggtgg tgcgcacctt ctatctcagc
     2401 aacgccgacc tgaaggagac gatcgacctg ctccggatgg tggtcgacct gcggcgcctg
     2461 gcgccgatca ccgcgaccaa cgcgatttcg atcaaggaca cgcccgagcg gatcgaggca
     2521 gccgcccgcc tcatcggcgc catcgacaag gcgcgcccgg aggtagtgaa cgacgtcgag
//
LOCUS       DAOPQX010000436         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_44256_length_2550_cov_4.120641, whole genome shotgun sequence.
ACCESSION   DAOPQX010000436 DAOPQX010000000
VERSION     DAOPQX010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44256_length_2550_cov_4.120641"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKK95_16275"
     CDS             <1..658
                     /locus_tag="PKK95_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19827.1"
                     /translation="VVLRPAGSREEIWVALFHIPWFPSALVRLDAKGKVTGEFWHPGY
                     VTAVVPATIGGREVLVVGASNNDFRSSALAVVDPDVPTTAPALRPDYACADCPGGRPL
                     HYLVFPRTTLARQFDPVGAIQAITVDAAGQMVVAVEQLGVSLTGMATPAQASVHYVFD
                     SNMRPVSAELGETYVRAYRELLAQKRFTAPPNFDEQSELWPVRKWDGSAFVPLQPPPR
                     "
     gene            complement(812..2200)
                     /locus_tag="PKK95_16280"
     CDS             complement(812..2200)
                     /locus_tag="PKK95_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19828.1"
                     /translation="MNALPFLAILLGVASVAHPPATGGQAAVIGSPGRPATSRAIELR
                     VTGVRVLPSIGNRTASAGREFVIVETAWKNLVPKKAVNRKKAADRTAGAGSLGFGGGA
                     TAQDKAAASADTTLEDVKFEARPLPNLLWLIVDGRVAEPIDVQATRGLPGHVGPDGLS
                     IPGFEQVVSGGLAFETQAGAKSLALLFLDSVNGHLLVPISGPVPALATGLGGSARANE
                     IVELAATGATWADAKDPGRRRLVVTLRGISRKEALVDLPFADFGFLLTDQGCVFSPDR
                     DAAGLTRPLAPRGRFTPFVPSEGQLAFSVPAATRAAALLFRPRQGAAIDLPVLGEVKP
                     KWPAPEATITDGDVLRVLKLPGTAVPPGLPPPAAGAEQVALDLVVENLRTGAGIELQA
                     AQQFRLVGPGDARIVPSRASTQAPCRLDGRNVVPAGGSRRFTLVYDVPPGAPLQLEYR
                     GFNVKSELIKVR"
     gene            2247..>2550
                     /locus_tag="PKK95_16285"
     CDS             2247..>2550
                     /locus_tag="PKK95_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19829.1"
                     /translation="MRAAEIEGVSMDDASRPGQPAPLETVVTQGASGGRSGQFPHAGE
                     NLFAGLLLLLVADLVVETVLSRAGVRWVAAGIGLAGIVLAVAAWRRYRAAAAAWASV"
BASE COUNT          359 a          913 c          906 g          372 t
ORIGIN      
        1 ggtcgtcctg cgcccggcag gaagccgcga ggagatctgg gtggctctct tccatatccc
       61 gtggttcccg tcggcgctcg tgaggctcga cgcgaaaggg aaggtgacgg gcgagttctg
      121 gcacccgggg tacgtcaccg ccgtggtgcc ggccaccatc ggaggccgcg aggtgctggt
      181 cgtcggcgcc agcaacaacg acttccgcag ctcggccctc gcggtcgtgg acccggacgt
      241 tcccaccacg gcgccggccc tgcgccccga ctacgcctgt gccgactgtc ccggggggcg
      301 gcccctgcac tatctcgtct ttccacggac cacgctcgcg cgccagttcg acccggtggg
      361 ggcaatccag gcaatcaccg tggacgcggc cgggcagatg gtggtggcgg tcgagcagct
      421 gggcgtgtcg ctgaccggca tggcaacccc cgcgcaggcg tccgtgcatt acgtgttcga
      481 cagcaacatg cgcccggtct cggcggaact gggcgagacc tacgtcaggg cctaccgcga
      541 gctgctggcc cagaaacggt tcaccgcacc cccgaatttc gacgagcagt cggagctgtg
      601 gccggtgcgc aagtgggatg gctcggcgtt cgtcccgctt caaccgccgc cgcggtgaaa
      661 tgttgccagg ccccattcgc gtcacaagcg acgtaccccc accgcaccgt gcgaacggtg
      721 cagcggtggg agcgcgagct gcggcttccg attcaccggc tggccaccgg caagggcgat
      781 acggtctacg cgctggtgga cgacgctcct tctacctcac cttgatcagc tcgctcttga
      841 cgttgaagcc gcggtactcg agctgcagcg gcgcgccggg cgggacgtcg tagaccagcg
      901 tgaagcgccg cgagccgccg gccgggacca cgttgcggcc atcgagccgg cagggcgcct
      961 gcgtgctggc cctcgatgga acgatgcggg cgtcgcccgg ccccacgagg cggaactgct
     1021 gcgcggcctg cagctcgatg ccggcgcccg tgcgcaggtt ctcgacgacc aggtcgagcg
     1081 caacctgttc ggccccggcg gccggcgggg gcagcccggg cggtaccgcc gtgcccggca
     1141 gcttcaacac gcgaagcacg tcgccgtcgg tgatcgtcgc ctcgggcgcg ggccacttcg
     1201 gcttcacctc gcccagcacg ggcagatcga tcgcggcgcc ctgcctgggc cggaacagca
     1261 gcgcggcggc tcgcgtggcg gcgggaacgc tgaacgcgag ctgcccttcg ctcggcacga
     1321 acggggtgaa ccggccgcgc ggcgccagcg ggcgggtcag gcccgcggcg tcgcgatcgg
     1381 gtgagaagac gcacccctgg tccgtcagaa ggaagccgaa atccgcgaac ggcagatcga
     1441 cgagcgcttc cttgcggctg atgccgcgca gggtgacgac cagccgccgc cggcccgggt
     1501 ccttcgcgtc ggcccacgtc gcgcccgtcg cggccagctc cacgatctcg ttcgccctgg
     1561 ccgatcctcc gagaccggtc gcgagcgccg gcaccgggcc cgagatcggg acgagcaggt
     1621 ggccgttcac cgagtcgagg aacagcaagg cgagcgactt cgctcccgcc tgcgtctcga
     1681 acgccagccc gcccgacacc acctgttcga acccgggaat gctgagcccg tccggcccga
     1741 cgtggccggg cagcccgcgc gtcgcctgca cgtcgatcgg ttcggcgaca cggccgtcca
     1801 cgatcagcca gagcaggttc gggagaggcc gggcttcgaa cttcacgtcc tcgagggtgg
     1861 tgtccgccga cgccgcggcc ttgtcctgcg cggtcgcgcc gccgccgaag ccgagcgatc
     1921 ccgcgcccgc cgtgcggtcg gccgccttct tccggttcac ggccttcttc ggcaccaggt
     1981 tcttccacgc cgtctccacg atcacgaact cgcgcccggc agacgccgtg cgatttccaa
     2041 tcgacggcag aacccgcacg ccggtcacgc ggagttcgat cgccctgctg gttgccggcc
     2101 ggccgggtga gccgatgacg gcggcctgcc ccccggtcgc tggaggatga gccacagagg
     2161 cgacgcccag caggatggcg agaaagggaa gtgcgttcat ggcgctgcgg attctaactc
     2221 agccgcagtg ccgctcctct ggtaacatgc gcgcggccga aatcgagggg gtctcaatgg
     2281 acgacgcgtc tcgaccgggt cagccggcgc cgcttgaaac cgtggtgacg cagggggcga
     2341 gtggcgggcg gtcagggcaa ttcccgcacg cgggcgagaa cctgttcgcg ggcctgctgc
     2401 tcctcctcgt tgcggatctc gtcgtcgaga ccgtgctgtc acgcgccggt gtgcgatggg
     2461 tcgcggcagg catcggcctg gccggcatcg tgctcgccgt cgcagcgtgg cgccgctacc
     2521 gggcggccgc agcggcctgg gcctcggtct
//
LOCUS       DAOPQX010000437         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_44441_length_2543_cov_3.422026, whole genome shotgun sequence.
ACCESSION   DAOPQX010000437 DAOPQX010000000
VERSION     DAOPQX010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44441_length_2543_cov_3.422026"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PKK95_16290"
     CDS             complement(<1..698)
                     /locus_tag="PKK95_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOC19830.1"
                     /translation="MGQLILNVGDLEPQRDRRSSVLRSAGFEVIEAATGAEALAAARE
                     RRPDLVLLDVRLSDADGFGVCRAIKAGATGTPLVLHVSATFRDAAARIRALDNGADGC
                     LAEPVDADELVATARALLRLAAQGEARCKGASGEREASRRRAAEELRASRERLAQRTG
                     HLGVYDWDLATGEVVWSEELELVYGIPAPADPAERPAAWLRLVHPDDRDRLSRETTEW
                     LESTELEREWEYRLL"
     gene            complement(768..2030)
                     /locus_tag="PKK95_16295"
     CDS             complement(768..2030)
                     /locus_tag="PKK95_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC19831.1"
                     /translation="MTVERMLQAVPELKLGIENLRVHKLRSLLTMLGMIFGVAAVVSM
                     MSIGAGAQQQVMAFIEDLGVRNLIVEAREAGDNQTLDRVRKLSAGLSFQDLRVIRANV
                     SGIAASTARKRVTPTKMLPKPQGEMPVVYGVEPRYREIARLRVTEGRFFDAFDEERAA
                     PVCVLGQAARASLFGTATAVGEYVKANEQWYHVIGVVAPQAAAQTDVAGLPAQDRNNL
                     VYVPLQAALLRSEDSYSYLKDEIDGIYLQLEATTDPVGTASVVRSILDAAHRHAGDFT
                     VVVPAELLAQQRRTQRVFDMVMVALASISLLVGGIGIMNIMLATILERTREIGTRRAV
                     GARRRDIIRQFLVETILISLAGGLLGIVFGVGMSRLIAWLAGWSTIVTPASILLAFLV
                     SVSVGLIFGVYPALKAARLDPVEALHYE"
     gene            complement(2027..>2543)
                     /locus_tag="PKK95_16300"
     CDS             complement(2027..>2543)
                     /locus_tag="PKK95_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19832.1"
                     /translation="DTSGIELQAKVDEADRANVEAGDAAEVRVDGLPGGVFTGSVRTV
                     SGAAARGFFESESRRKFDTTLQLTAPDERLRAGLSASVVISGEQLKDVLYVPRQALFE
                     EDGKPVVYARRGGGFEARPVKVRFRSESRAVVEGVEAGVELALVEPGEDGKGAQNQAP
                     AGAAPGRGGGR"
BASE COUNT          374 a          969 c          847 g          353 t
ORIGIN      
        1 aggagccggt actcccactc gcgttcgagc tcggtcgatt ccagccactc ggtcgtttcg
       61 cgcgacagcc ggtctcgatc gtccgggtga acgaggcgca gccatgccgc ggggcgctcg
      121 gccggatcgg cgggcgccgg gatgccatag acgagctcga gctcctctga ccagacgacc
      181 tcaccggtcg ccagatccca gtcgtatacg ccgaggtggc ccgtccgctg cgcgagacgc
      241 tcccggctcg cccgcagctc ctcggccgcc cgccgacggc tcgcctcgcg ctcgcccgac
      301 gcccccttgc acctcgcctc gccctgggcg gccaggcgaa gcagcgccct ggccgtcgcg
      361 acgagctcgt ccgcgtcgac cggctcggcg aggcagccgt cggcgccgtt gtcgagggcg
      421 cgaatccgcg cggcggcgtc gcggaacgtg gccgagacgt gcagaaccag cggcgttccc
      481 gtcgcgccgg ccttgatggc ccggcagacg ccgaacccgt cggcatcgga aaggcggacg
      541 tcgaggagga ccaggtcggg gcggcgctct cgcgcagccg ccagggcctc agcgccggtc
      601 gccgcctcga tcacctcgaa gccggcggag cggaggacgg acgatcgccg gtctctctgc
      661 ggctcgaggt caccgacgtt gaggatgagc tggcccacga gcgaacctcc gccccggcac
      721 tctgagaagt gaggacggca tcataccgtg gattcggagg aaggcagtca ctcgtagtgc
      781 agcgcctcca ccgggtcgag gcgagccgcc ttgagcgccg ggtagacgcc gaagatcaac
      841 cccaccgaca ccgacaccag gaaggccagg aggatcgatg ccggcgtgac gatggtggac
      901 caccccgcca gccaggcgat gagccgcgac atgcccacgc cgaagacgat gcccagcagg
      961 ccgcccgcca gcgagatgag gatcgtctcg accaggaact ggcggatgat gtcgcggcgg
     1021 cgggcgccga cggcccggcg cgtcccgatc tcgcgggtgc gctcgaggat ggtcgccagc
     1081 atgatgttca tgatcccgat gccgccgacc agcagcgaga tcgaggcgag cgccaccatg
     1141 accatgtcga acacgcgctg ggtgcggcgc tgctgggcca gcagctcggc cggcacgacg
     1201 accgtgaagt cgccggcgtg ccggtgggcc gcgtccagga tgctgcggac gaccgaggcg
     1261 gtccccacgg gatcagtcgt ggcctccagc tgcaggtaga tgccgtcgat ctcgtccttc
     1321 aggtacgagt acgagtcctc cgagcgcagc aatgcggcct gcagtggcac gtacaccagg
     1381 ttgttgcggt cctgtgccgg aaggccagcg acgtcggtct gcgccgcggc ctgcggcgcc
     1441 accacgccga tgacgtggta ccactgctcg ttggccttca cgtactcccc caccgcggtc
     1501 gccgtcccga acaggctcgc ccgcgcggcc tgccccagca cgcacaccgg cgcggcccgt
     1561 tcctcgtcga acgcgtcgaa gaaccgcccc tcggtcaccc gcaggcgcgc gatctcgcgg
     1621 tagcggggct cgacgccgta gacgaccggc atctcgccct gcggcttcgg cagcatcttg
     1681 gtgggcgtca cccgcttgcg cgcggtggac gccgcgatgc cgctcacgtt cgcccggatc
     1741 acgcggaggt cctggaacga caacccggcc gacagcttgc gcacccgatc gagcgtctgg
     1801 ttgtcgcccg cctcgcgcgc ttcgacgatc aggttgcgga cgccgaggtc ctcgatgaag
     1861 gccatcacct gctgctgggc gccagcgccg atcgacatca tcgacaccac cgcggccacg
     1921 ccgaagatca tgccgagcat ggtcagaagc gaccgcagct tgtgcacgcg gaggttctcg
     1981 atgcccagct tcagctcggg caccgcctgc agcatccgtt cgacggtcac cgcccccctc
     2041 cccgccctgg cgccgcgccg gcaggtgcct ggttctgcgc gcccttgccg tcctcgcccg
     2101 gctcgaccag cgccagctcg acacccgcct cgaccccctc gacgaccgcg cggctctccg
     2161 agcggaaccg caccttcacc ggcctcgcct cgaacccgcc cccgcggcgg gcgtacacca
     2221 ccggcttccc gtcctcctcg aacagcgcct ggcgcggcac gtacaagacg tccttcagct
     2281 gctcgcccga gatgacgacg cttgccgaga gccccgcccg gagccgctcg tcgggcgcgg
     2341 tcagctggag cgtcgtgtcg aacttcctcc tcgactcgct ctcgaagaag ccccgggccg
     2401 ccgcgccgct gacggttcgc acgctgccgg tgaagacgcc gccgggcagc ccgtccaccc
     2461 gcacctcggc ggcatccccg gcctcgacgt tggcgcgatc ggcctcgtcc accttcgcct
     2521 gcagttcgat gccgctggtg tcg
//
LOCUS       DAOPQX010000438         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_44673_length_2532_cov_4.469116, whole genome shotgun sequence.
ACCESSION   DAOPQX010000438 DAOPQX010000000
VERSION     DAOPQX010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44673_length_2532_cov_4.469116"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /gene="rnr"
                     /locus_tag="PKK95_16305"
     CDS             <1..1480
                     /gene="rnr"
                     /locus_tag="PKK95_16305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOC19833.1"
                     /translation="TIDGESARDFDDAISVERLPNGHYHLGVHIADVSHYVTEGSALD
                     TEAYERGTSVYFPERALHMFPEELATGLCSLNPRVDRLVQSCLMEVDRHGQVVRSGFH
                     DGIIRTVERMTYTDVNAILTGESPETTARYETLVPLFERMRDLFQLLHDRRRRLGSID
                     FDLSESRVVLGPDGQIEAIVASERNVAHRIIEEFMLLANEAVAEYLEASGVPGLYRVH
                     EEPDPLKVVRFEEFISTLGYSLGVPPHTARPRHFQALVRKIRGKPEEKPIAALMLRTM
                     QKARYAPENLGHFGLAFSSYTHFTSPIRRYPDLVVHRLLRAARQGGLGEARHEELAED
                     LPEIARHTSDRERRADEAERELLQWKKVRFMADKVGDEFDGFITGVAPFGLFIELVEH
                     FVEGLVHVSTMADDYYRFLEATHSLRGENTHKVYRLGDKVRARVIRVDLEKRQIELGL
                     SEILDAVRQEERRRAPRRPPRRSKAEGRNEPLRKRPRTRKRR"
     gene            1502..>2532
                     /gene="selB"
                     /locus_tag="PKK95_16310"
     CDS             1502..>2532
                     /gene="selB"
                     /locus_tag="PKK95_16310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOC19834.1"
                     /translation="MRHLIFGTAGHIDHGKSALVRALTGTDPDRLKEEKARGITIDLG
                     FAHWADGEVNFSFVDVPGHERFVRNMLAGVGGIDAVVLVVAADESVMPQTREHFEICR
                     LLHVPAGLVALTKADLVDEEMLELVRLETRELVAGSFLEDAPVVPVSSKTGAGLDDLR
                     RELRALAGRVEPRRPRGVARLPIDRVFSVKGFGTVVTGTLVSGAVELESELSLLPSGR
                     PVKVRGVQVHGAKEAVAVAGQRAAVNLSGVDLDEVHRGQTLVTPGTLLPTRAIDARLQ
                     LLPSSRALKHGARVRFHQGTDEVLGRVALAAVLPAPGRDGPAPPGQGADVPPGADAYV
                     RVRLEAPAVL"
BASE COUNT          384 a          883 c          909 g          356 t
ORIGIN      
        1 gaccatcgac ggcgagagcg cgcgcgactt cgacgacgcc atctcggtgg aacggctgcc
       61 gaatggccac tatcacctcg gcgtgcacat cgccgacgtc tcgcactacg tgaccgaggg
      121 gagcgccctc gacaccgagg cgtacgagcg ggggacgtcg gtgtactttc ccgagcgcgc
      181 gctgcacatg ttccccgagg agctggcgac ggggctctgc agcctgaacc cgcgggtcga
      241 tcgcctggtg cagtcgtgcc tgatggaggt ggaccgccac ggccaggtcg tgcgctccgg
      301 gttccacgac gggatcatcc ggaccgtcga gcggatgacc tataccgacg tgaacgcgat
      361 cctgacgggg gagagccccg aaacgactgc ccggtacgag accctggtgc cgctgttcga
      421 gcggatgcgc gacctcttcc agctgctcca cgaccggcgg cggcgcctcg gctcgatcga
      481 cttcgacctg agcgagtcgc gggtcgtgct cgggcccgac ggccagatcg aggcgatcgt
      541 cgccagcgag cggaacgtcg cccaccggat catcgaggag ttcatgctcc tcgccaacga
      601 ggcggtggcc gagtacctcg aggccagcgg ggtgcccggg ttgtaccggg ttcacgaaga
      661 gcccgacccg ctgaaggtgg tccgcttcga ggagttcatc tccacgctcg ggtacagcct
      721 gggggtgccg ccgcacaccg cccgcccccg gcacttccag gcgctggtgc ggaagatccg
      781 gggcaagccc gaggagaagc cgattgccgc gctgatgctg cggacgatgc agaaggcgcg
      841 gtacgcgccc gagaatcttg gacacttcgg cctcgccttc agcagctaca cgcacttcac
      901 ctcgccaatc cgccgctacc ccgacctggt cgtccaccgc ctgctgcgtg ccgcgcgcca
      961 gggagggctg ggggaggccc ggcacgagga gctcgctgaa gacctgcccg aaatcgcccg
     1021 ccacacctcc gaccgcgagc ggcgcgccga cgaggccgag cgcgagctgc tgcagtggaa
     1081 gaaggtgcgg ttcatggccg acaaggttgg cgacgagttc gacggcttca tcaccggcgt
     1141 cgccccgttc ggcctcttca tcgagctggt cgagcacttc gtcgaagggc tcgtccacgt
     1201 gtccacgatg gccgacgact actaccggtt cctcgaggcc acgcactccc tccgcggcga
     1261 gaacacgcac aaggtctacc ggctcggcga caaggttcgg gcgcgggtga tccgggtcga
     1321 cctcgagaag cggcagatcg agctcgggct ctccgagatc ctcgacgccg tgcggcagga
     1381 ggagcggcgc cgcgcgccgc ggcgcccgcc gcggcgctcc aaggccgagg gccgcaacga
     1441 gcccctccgc aagcgcccgc ggacgcggaa gagacgatga ccgcaggcag cgggcaatga
     1501 gatgcggcac ctgatcttcg gcaccgccgg ccacatcgac cacggcaaga gcgctctcgt
     1561 gcgcgcgctg accgggaccg accccgaccg gctgaaagaa gagaaggcac gcggcatcac
     1621 gatcgatctc ggcttcgcgc actgggcgga cggcgaggtg aacttctcgt tcgtcgacgt
     1681 cccagggcac gagcgtttcg tcaggaacat gctcgccggc gtcgggggga tcgacgcggt
     1741 ggtgctggtc gtggccgccg acgagtcggt gatgccgcag acgcgcgagc acttcgagat
     1801 ctgccgcctg cttcacgtgc ccgccggcct ggtggcgctc accaaggccg acctggtgga
     1861 cgaggaaatg ctggagctgg tccgcctgga gacccgtgag ctggtggcag gatcgttcct
     1921 ggaggatgcc ccggtcgtgc cggtgtcgtc gaagaccggc gccggcctgg acgatttacg
     1981 ccgcgagctg cgggccctgg cagggcgcgt cgagccgcgc cggccccggg gcgtggcgcg
     2041 actcccgatc gaccgggtct tctcggtgaa gggcttcggc acggtggtga ccgggacgct
     2101 ggtttccggg gccgtggaac tcgaatcgga gctgtcgctt ctaccctccg gccgcccggt
     2161 caaggtgcgc ggcgtccagg tgcacggcgc caaggaggcg gtggcggtgg cggggcagcg
     2221 tgcggccgtg aatctcagcg gcgtggacct cgacgaggtt catcgcggcc agacgctcgt
     2281 gacgccggga accctcctgc ccacgcgcgc gatcgacgcc cggctgcagc tgctgccctc
     2341 gtcgcgcgcg ctgaagcacg gcgcccgcgt ccgcttccac cagggaaccg acgaggtgtt
     2401 gggccgcgtc gccctcgcgg ccgtgctgcc cgcgcccggc cgggacggcc ccgcgccgcc
     2461 cggacagggc gccgacgtgc cgcccggcgc cgacgcctac gtgcgggtgc gcctcgaggc
     2521 accggccgtg ct
//
LOCUS       DAOPQX010000439         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_45655_length_2490_cov_4.916632, whole genome shotgun sequence.
ACCESSION   DAOPQX010000439 DAOPQX010000000
VERSION     DAOPQX010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45655_length_2490_cov_4.916632"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1555
                     /locus_tag="PKK95_16315"
     CDS             155..1555
                     /locus_tag="PKK95_16315"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOC19835.1"
                     /translation="MNQPTRIDRRTMLRTAAAAAAGFSIIPRHVVAGSGRPAPSDTLR
                     IAIIGTGGQGVVNLKALFQEDDVRVIAIADPNEESDLRAWYYGGSAGRLPTQKLIDDT
                     YRQKDGAAFKPVQAYVDFREMLEKEKEIDAVLVATPDHLHGYVSLAVLQLKKHLYCEK
                     PLCRSIYEVQKVVAAAKAAGVATQMGNYGHSGEGLRLFCEWIQDGAIGPVREVHAWAT
                     FPPLNTFKEQPKETVPTPPGLNWDLWLGPAAERPYHPAYTPVTWRPWHDFGTGMMGDF
                     ACHHLDPAFMALELGYPDTVEAVHYGAGKETYPFAATIYMEFPARGERPPVKVTWYDN
                     GLRPPTPEELGATRRLGPHGILMVGDKGKILGGGWSQSPRLIPEEAMQAYKRPPRTLR
                     RVKGHHRDWIDACKGGEPSSANFETVGRMVEVVLMGAIAERIGEKLHWDQAAGRFTSA
                     RANELVTPTFRKGWEV"
     gene            1697..>2490
                     /locus_tag="PKK95_16320"
     CDS             1697..>2490
                     /locus_tag="PKK95_16320"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOC19836.1"
                     /translation="MRPSVRLALVLGGVVAASALFAPSAEAQWRARSRVRVFVGAHIG
                     HPHFYYRPFYDPFFYRGFYGWAPYAWPGAYPQWGRARVDRNAVLVRPDVSPDEAQVYV
                     DGYFRGTASDLDGRFEGLVVEPGEHEIVLYLPGYRTARRTVELRGGTVYRLREKLVKL
                     PEGEQSEPPPPPSPRSEQAPPSRPGGQAPPPPPPERYEPLPGRPAPDQPRRVPRPPTE
                     ELRERPDLEARGFGSIAIRVQPQDAEILIDGERWEGPETGRPLEVLL"
BASE COUNT          393 a          884 c          860 g          353 t
ORIGIN      
        1 ttgtcgaacc gcaggcgagc gggggccctc cgccccgcgt cacccgctca cggccttgct
       61 caacgcaaca cgttctccgc tacacgaccc ccatccagcg tcctgctagg ctatactccg
      121 cccgtcagca acattccgcc cgtcaggagc catcatgaac cagcctacgc ggatcgatcg
      181 gcgaaccatg ctcagaaccg cggccgcggc cgccgccggt ttctccatca tccccaggca
      241 cgtggtggcc gggtcgggcc gccccgcccc gagcgacacc ctgcggatcg ccatcatcgg
      301 caccggcggg cagggggtcg tcaacctgaa ggcgctgttc caggaagacg atgtgcgggt
      361 gatcgcgatt gcggatccca acgaggagag cgacctgcgc gcctggtatt acggcggctc
      421 cgccgggcgc cttcccacgc agaagctgat cgacgacacc taccggcaga aggatggcgc
      481 cgcgttcaag ccggtccagg cctacgtcga cttccgcgag atgctggaga aggagaagga
      541 gatcgacgcg gtgctggtgg cgacccccga ccacctgcac gggtacgtga gcctcgcggt
      601 gctgcagctg aagaagcacc tgtactgcga gaagccgctc tgccggtcca tctacgaggt
      661 gcagaaggtg gtggcggcgg ccaaggcagc gggcgtcgcg acgcagatgg gcaactacgg
      721 ccacagcggc gaggggctgc ggctgttctg cgagtggatc caggacggcg cgatcggccc
      781 cgtgcgcgag gtgcacgcgt gggcgacgtt cccgcccctc aacacgttca aggaacagcc
      841 gaaggaaacg gtgccgacgc ctccgggcct gaactgggac ctgtggctcg ggccggcggc
      901 cgaacgcccg tatcaccctg cctacacccc cgtgacctgg cgcccgtggc acgacttcgg
      961 gaccgggatg atgggcgact tcgcgtgcca ccacctcgac ccggcgttca tggcgctcga
     1021 gctcggctac cccgacacgg tggaggccgt ccactatggc gcaggcaagg agacgtaccc
     1081 gttcgcggca acgatctaca tggagttccc cgcccgcggc gagcgtccgc cggtcaaggt
     1141 cacgtggtac gacaacgggc tgaggccgcc cacgcccgag gagctgggag ccacgcggcg
     1201 tctcggcccg cacggcatcc tcatggttgg cgacaagggg aagattctcg gcggaggctg
     1261 gtcgcagtcg ccgcgcctga tccccgagga agcgatgcag gcctacaagc ggccgccgcg
     1321 gaccctgcgc cgcgtgaagg gacaccatcg cgactggatc gacgcctgca agggcgggga
     1381 gccctcgagc gccaacttcg agaccgtcgg ccggatggtg gaggtggtgc tgatgggcgc
     1441 gatcgccgag cgcatcggcg agaagcttca ctgggaccag gcggccgggc gcttcacgtc
     1501 ggcgcgcgcc aacgaactgg tgacgcccac cttccggaag ggatgggaag tgtagacggc
     1561 ccaggcccct gtcgccggct ggtgacaacg gcggccgctc gtcggccgcc tgccgcctga
     1621 tcgcgctaaa actggcctgt ttttacccct tcgccactgg cgcgccgctt gctccggctg
     1681 agtcggaggc attgtcatgc gaccgtcagt cagattggcg ctggtgctgg gcggcgtcgt
     1741 tgccgcgtct gccctgttcg ccccgtccgc agaggcgcag tggcgcgcgc gcagccgggt
     1801 tcgtgtcttc gtcggcgcgc acatcggcca cccccacttc tactaccgcc cgttctacga
     1861 cccgttcttc taccgcggtt tctacggctg ggccccgtac gcctggcccg gtgcctaccc
     1921 gcagtggggc cgggcgcgcg tcgatcgcaa cgccgtcctg gttcggcccg acgtctcgcc
     1981 agacgaggcg caggtctacg tggacggcta tttccgcggg acggcgagcg atctcgacgg
     2041 gcgtttcgag gggctggtcg tcgagcccgg cgagcacgag atcgtgctct acctgccggg
     2101 ttaccgcacc gcgaggcgca ccgtcgagct gaggggcggc acggtgtacc gcctgaggga
     2161 aaaactggtg aagctgccgg agggcgagca gagcgaaccg cccccgccgc cgtcgccgcg
     2221 gagcgaacag gcgcccccat cgcgaccggg cggacaagcg ccgccgccac cgccccccga
     2281 gcgctacgag ccgctgcccg gacgcccggc ccccgaccag ccgcggcgtg tgccgaggcc
     2341 gcctaccgaa gagctgcgcg agcgccccga cctcgaagcg cgcggcttcg gctcaatcgc
     2401 catccgcgtg cagccccagg atgccgagat cctgatcgac ggtgagcggt gggaaggccc
     2461 cgagaccggc cggccgctcg aggtcctgct
//
LOCUS       DAOPQX010000440         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_46160_length_2471_cov_3.949089, whole genome shotgun sequence.
ACCESSION   DAOPQX010000440 DAOPQX010000000
VERSION     DAOPQX010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46160_length_2471_cov_3.949089"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="PKK95_16325"
     CDS             <1..1501
                     /locus_tag="PKK95_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOC19837.1"
                     /translation="RTQELEPWLAESWSTSPDGLEWTLKLRQGVTFSDGEPFTAADVL
                     FSFRAIYDPGVASPMGEAMRVGGKPLEVSAPDPSTVVIRFPAPFGPGIRLLDNLPILP
                     RHKLEAALDAGTFVKAWDGAAPVGDIVGLGPFVIEQYQPGQRLVFARNPRYWRRDESG
                     QRLPALDRLTLEIVPDQNAELLRLQAGQIDFTQTEVRPEDYATLRAAADEGRVTLVDL
                     GVGLDADSFWINLRPGTNQARPWLHQVELRRAIAHAVDRQAFADTVFLGAATPVDGAL
                     ITPGNPRWYATDLPRHPYDPGAAGALLASIGLVDRDGDGVREDRHGADASFTVLTMKG
                     NSLLERGAAFVRDALGAIGLRVEVAPLEVGALIDRIERGDYEAIYFRFLTTDLDPALN
                     MDLWLSSGGAHVWNPLQRTPATDWEREIDRLMHRQAATPDHAARKALFDQVQRIFAEH
                     VPVLQFAAPRIFVALSARVANATPALLRPAVVLWNPDRLDVREGKRASR"
     gene            1509..2468
                     /locus_tag="PKK95_16330"
     CDS             1509..2468
                     /locus_tag="PKK95_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOC19838.1"
                     /translation="MRGYLARRVLFAAMLVFLVSSAAMVLTRLAPGDFASIVAAERAS
                     PGQVAQERARYGLDRPIASHYFDWLLRAVRLDLGDSLLYSRPVGALVRERAANTALLA
                     LAALALATLAGLPAGVYSGSRRGAGASVVRGASIVLLSLPSLVTSLVLVLVAARTGWF
                     PVGGMVSGASTGAGVAGWWADVAWHLPLPALALALPLAATLERLQSQAMADALGQPFT
                     LAAVARGVPRRRVVWRHALRASLSPVASVYGYVLAALLSGSFIVEVITSWPGLGRLLY
                     DGLRARDVYLVAGCAAAGSLFLAIGSLVSDLALAWADPRMRRG"
BASE COUNT          282 a          938 c          872 g          379 t
ORIGIN      
        1 ccgcacccag gagttggagc cctggctggc cgagtcgtgg agcacctctc cggacggcct
       61 cgagtggacg ctcaagctgc ggcagggagt gaccttctcc gacggcgagc ccttcaccgc
      121 cgccgacgtg ctcttctcgt tccgggccat ctacgatccg ggcgtggcca gtcccatggg
      181 cgaggccatg cgcgtcggcg ggaagcccct tgaggtcagc gcccccgacc cgtccaccgt
      241 cgtcatccgc tttccggcgc ccttcggccc cggaattcgg ctgctcgaca acctgccgat
      301 cctgccgcgc cacaagctcg aggccgccct cgacgcgggc accttcgtca aggcgtggga
      361 cggcgctgcg ccggtcggcg acatcgtcgg cctcgggccg ttcgtcatcg agcagtacca
      421 gcccgggcag cgcctcgtct tcgcccgcaa cccgcgctac tggcgccggg acgagtcggg
      481 gcagcggctg ccggccctcg accgtctcac cctcgagatc gtccccgacc agaacgccga
      541 gctgctgcgc ctgcaggccg gccagatcga cttcacgcag accgaggtcc ggcccgagga
      601 ctacgccacc ctgagggcgg ccgccgacga gggccgcgtc accctcgtcg acctgggcgt
      661 gggcctcgac gccgactcgt tctggatcaa cctgcggccc gggaccaacc aggcgcgccc
      721 ctggcttcac caggtcgagc tgcggagggc catcgcgcac gcggtcgacc ggcaggcctt
      781 cgccgacacg gtgttcctcg gcgcggcgac cccggtggac ggggccctga tcacgcccgg
      841 gaatccccgc tggtacgcaa ccgacctccc gcgccacccc tacgacccgg gcgcagccgg
      901 cgcgctgctc gcgtcgatcg gcctggttga tcgcgacggc gacggcgtcc gcgaagaccg
      961 gcacggcgcc gacgcctcgt tcaccgtgct gacgatgaag gggaacagcc tgctcgagag
     1021 gggcgccgcg ttcgtgcgcg acgcgctcgg cgcgatcggc ctccgcgtcg aggtggcccc
     1081 gctcgaggtg ggcgcgctga tcgaccggat cgagcggggc gactacgagg ccatctactt
     1141 ccgcttcctc acgaccgatc tcgacccggc cctgaacatg gacctgtggc tcagctccgg
     1201 cggcgcgcac gtgtggaacc cgctgcagcg gacgccggcg accgactggg agcgcgagat
     1261 cgaccgcctg atgcaccggc aggcggccac gcccgaccac gccgcgcgca aggcgctctt
     1321 cgaccaggtg cagcggatct tcgccgagca cgtgccggtg ctgcagttcg cggcgccgcg
     1381 catcttcgtg gcgctcagcg cgcgcgtcgc caacgccacc cccgcgctgc tgcgcccggc
     1441 cgtcgtcttg tggaaccccg accggctcga cgtgagggaa gggaagcggg cgtcccggtg
     1501 aggcgttggt gagaggctac ctggcgcggc gcgtgctctt cgccgccatg ctcgttttcc
     1561 tcgtgtcgtc ggcggccatg gtgctgacgc gcctggcgcc gggcgacttc gcctcgatcg
     1621 tggctgccga gcgcgccagc ccgggacagg tcgcgcaaga gcgggcgcgg tacgggctcg
     1681 accgcccgat cgcctcgcac tacttcgact ggctgttgag ggccgtccgc ctcgatctgg
     1741 gcgactcgct gctgtacagt cggccggtcg gagccctggt ccgcgagcgc gcggccaata
     1801 ccgcgctgct cgcgcttgcc gccctggcac tcgccacgct tgccgggctt cccgccggcg
     1861 tctactccgg cagccgccgg ggggctggcg cgtcggtcgt gcgcggggcc tcgatcgtcc
     1921 tgctgtcgct gccctcgctg gtcacctcgc tcgtgctcgt cctcgtggcg gcgcgtaccg
     1981 gctggttccc ggtcggcggg atggtttccg gcgcctcgac gggagcgggc gtggccggct
     2041 ggtgggccga cgtggcgtgg caccttccgc tgcccgccct cgcgctcgcc ctgccgctgg
     2101 cagccaccct cgaacggctg cagtcgcagg cgatggccga cgcgctcggc cagccgttca
     2161 ccctcgcggc ggtcgcgcgg ggcgtgccgc gccgccgggt cgtctggcgg cacgccctgc
     2221 gagcctcact gtcgccggtc gcctcggtct acggctacgt gctggccgcc ctcctcagcg
     2281 gttccttcat cgtcgaggtg attacctcgt ggccggggct ggggcggctc ttgtacgacg
     2341 gactgcgcgc ccgcgacgtc tacctggttg ccggctgcgc cgccgccggg tcgctgttcc
     2401 tcgcgatcgg aagcctggtc tcggacctgg cactcgcgtg ggccgacccg cggatgagga
     2461 gagggtaggg c
//
LOCUS       DAOPQX010000441         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_47225_length_2429_cov_3.526116, whole genome shotgun sequence.
ACCESSION   DAOPQX010000441 DAOPQX010000000
VERSION     DAOPQX010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47225_length_2429_cov_3.526116"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKK95_16335"
     CDS             <1..442
                     /locus_tag="PKK95_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOC19839.1"
                     /translation="INQLREKIGVMFGNPETTTGGRALKFYASVRVDIRRIASIKDGD
                     AIIGGRTRVKVVKNKVAPPFREAEFDILYGEGISREGDLLDLAVDRKIVDKSGTWFAY
                     NGERLGQGRENVKQFLRDNPEIAQAIEDRVRKELGLTREESAAV"
     gene            complement(521..>2429)
                     /locus_tag="PKK95_16340"
     CDS             complement(521..>2429)
                     /locus_tag="PKK95_16340"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC19840.1"
                     /translation="SYGEDGGQELTNLAFSGDGAFVVYVRGGDHGANWAAEGGLAPNP
                     ASSPVQARMQVWAVPVAGGQPVPLGEGDGPVPAPRSRQVAFVRGGQIWTVEIGGEASA
                     LFFARGRNHSPAWSPDGRTLAFVSDRDDHSLIGLYTGPNAPIRYLAPSTSRDTRPVWA
                     PDGRRIAFVRQPGRGGATRPPLGQAPQPWTIVVADVQTGAGREVWRSGGSLADSIPRT
                     LGGPNLNWADGNRLVFLSYHDGWPHLYSVSAEGGSRPLLLTPGRFMVEWVAMAPDRRS
                     VLYNANAGTEAHDVDRRHLFRVPVDRPEPAPLSTGLGIEWSPLLTADGRWLAYLASDA
                     RNAATLKVRPVGGGDVVSVTSGLVPGDFPADSLVVPEPVVVKSPDGLDIHCQVFRTPS
                     GPARRPAIVFAHGGPPRQMLLGWHYGFYYSNTYALNQFLASRGFLVLSVNYRLGIGYG
                     HDFHYPERAGSRGASEYQDVLAAGRYLQSRPDVDPERVGIYGGSYGGYLTALALARNS
                     DVFAAGVDIHGVHSWLRPPSEEVLAAAKVGDGLAEDDLLQAARVAWASSPASSVGTWR
                     SPVLLVHGDDDRNVQFAQTTDLVRRLAAAGVTYEEIVIPDDIHDFLLHRNWVRVGEVT
                     AEFFERHLGGK"
BASE COUNT          397 a          848 c          870 g          314 t
ORIGIN      
        1 catcaaccag ctgcgcgaga agatcggcgt gatgttcggc aaccccgaaa ccaccacggg
       61 cgggcgcgca ctgaagttct acgcgtcggt gcgcgtcgac atccgccgca tcgccagcat
      121 caaggacggc gatgcgatca tcggcggccg cacgcgggtg aaggtcgtga agaacaaggt
      181 cgccccgccg ttccgcgagg ccgagttcga catcctctac ggcgagggaa tctcgcgcga
      241 gggtgacctg ctcgacctgg ccgtcgatcg caagatcgtg gacaagagcg gcacctggtt
      301 cgcctacaac ggcgagcgcc tcggccaggg gcgcgagaac gtcaagcagt tcctccgcga
      361 caaccccgag atcgcccagg cgatcgaaga ccgcgtgcgc aaagagctcg gactgacgcg
      421 cgaggagtcg gcggcggtct gacgccagga atcaggagtc gggaatcagg attcaggggt
      481 cctggcgcag ggaacgagca ccgggggctc gccctgcgcc tcacttccct cccaagtgcc
      541 gctcgaagaa ctccgccgtc acctccccga cccgcaccca gttgcggtgc agcaggaagt
      601 cgtggatgtc gtcagggatg acgatctctt cgtacgtcac gccggcggcc gcgaggcgcc
      661 tcaccaggtc cgtcgtctgg gcgaactgca cgttccggtc gtcgtccccg tgcacgagca
      721 ggaccggcga gcgccaggtg cccaccgaag acgcgggcga tgacgcccac gccacgcggg
      781 cggcctgaag gagatcatcc tcggcaaggc cgtcgcccac cttcgcggcc gccagcacct
      841 cctcagaggg aggccgcaac cagctgtgga cgccgtgaat gtcgacgccg gccgcgaaca
      901 cgtcggagtt cctggcgagc gcgagcgcgg tgaggtagcc gccgtacgaa ccgccgtaga
      961 tcccgacccg ctcaggatcg acgtccggtc gcgactggag gtagcgcccg gccgccagca
     1021 cgtcctggta ctcggaggct ccccggctgc ccgcgcgttc ggggtaatgg aagtcgtggc
     1081 cgtagccgat gcccaggcgg tagttcaccg acagcacgag gaaccctcgg ctcgcaagga
     1141 actggttcag cgcgtacgtg ttcgagtagt agaagccgta gtgccagccg agcagcatct
     1201 ggcgcggcgg cccgccgtgc gcgaagacga tggccggccg gcgcgcaggc ccgcttgggg
     1261 tgcggaacac ctggcagtgg atgtcgaggc cgtcgggcga cttcacgacg accggctcgg
     1321 gcacgacgag gctgtcggcc gggaaatcgc ctgggacaag gccgctcgtc accgaaacga
     1381 cgtcgccgcc gccgacaggg cggaccttga gcgtggcggc gtttcgcgcg tcggaagcca
     1441 ggtaggccag ccaccgcccg tccgcggtga ggagcggcga ccactcgatg ccgaggccgg
     1501 tcgaaagcgg cgccggctcc ggacggtcca ccggaacccg gaagaggtgg cggcggtcga
     1561 cgtcgtgcgc ctcggtcccg gcgttcgcgt tgtagaggac gctgcgccgg tcgggtgcca
     1621 tcgccaccca ctccaccatg aagcggcccg gggtcagaag cagcggcctc gagccacctt
     1681 cggccgacac cgagtagagg tgcggccagc cgtcgtggta cgagaggaag acgaggcggt
     1741 tgccgtccgc ccagttcagg ttgggaccgc cgagcgttcg ggggatcgag tcggccagcg
     1801 acccgccgct gcgccacacc tcgcggcccg cgccggtctg cacgtcggcc acgacaatcg
     1861 tccagggctg cggcgcctgc ccgagcggcg gccgggtggc gccgccccgg cccggctggc
     1921 gcacgaaggc gatgcgccgg ccgtcgggcg cccacaccgg acgcgtgtcg cgcgaggtgc
     1981 tcggggcaag gtagcggatt ggcgcgttcg gtcccgtata gaggccgatg aggctgtggt
     2041 cgtcgcgatc ggagacgaac gccagcgtgc gcccgtcggg cgaccaggcg ggcgagtggt
     2101 tccggccgcg ggcgaagaag agcgccgagg cttcgccacc gatctcaacc gtccagatct
     2161 ggccgccacg cacgaaggcc acctgcctcg accggggtgc aggcaccgga ccgtcgccct
     2221 ctcccagcgg caccggctgg ccgccggcaa ccggcacggc ccagacctgc atcctggcct
     2281 ggacgggact gctcgcgggg ttcggggcca ggccgccttc tgccgcccag ttcgccccgt
     2341 gatccccgcc cctcacgtag acgacgaagg cgccgtcgcc cgagaacgcg aggttggtca
     2401 gctcctgccc gccgtcctcg ccgtaggag
//
LOCUS       DAOPQX010000442         2375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_48612_length_2375_cov_3.705603, whole genome shotgun sequence.
ACCESSION   DAOPQX010000442 DAOPQX010000000
VERSION     DAOPQX010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48612_length_2375_cov_3.705603"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /gene="ruvB"
                     /locus_tag="PKK95_16345"
     CDS             <1..909
                     /gene="ruvB"
                     /locus_tag="PKK95_16345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOC19841.1"
                     /translation="ENLLVSITAARQRNEALDHVLLYGPPGLGKTTLAYVIGNELGVP
                     VRSTSGPVLERPGDLAAMLTNLKEREVLFIDEIHRMAATIEEILYPAMEDYQLDIMIG
                     QGPSARSVKVPLPRFTLIGSTTRAGLLTSPLRGRFGIVHRLDFYTASDIREIVIRSAR
                     ILGVTLQPGAAEEIAGRSRGTPRVANRLLRRVRDYAQVRAQGEITPDVARAGLKLLEV
                     DEHGFDEIDRRLLRTIIDKFGGGPVGLNTIAAAISEEKDAIEDIYEPFLIQIGFLDRT
                     PRGRVATARAYEYFGLRTPDRDHRLW"
     gene            927..1973
                     /locus_tag="PKK95_16350"
     CDS             927..1973
                     /locus_tag="PKK95_16350"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOC19842.1"
                     /translation="MQKESQNAIPDHRPAGLGCGRPTEITALATHVPPRLLTNADFEK
                     MVDTTSEWILQRTGIVERHVVEPGVASSDLGAAAATKAIAQAGLTPDDIDLIIVGTTT
                     PDMFFPSTAALIQHKIGASHAWGFDLAGACSGFTFALATGSQLVSTGRHNHALIVGAD
                     VMSSIADYKDRATCVLFGDGAGAAVVSAAKDPSLAILDFAHEVDGSGAPFLQMPAGGS
                     LRPATHETVEQRLHYIKQDGQAVFKFAVRKTEEISRLIMQRNNLTPDDIDLFVSHQAN
                     MRIIKAAGERLGFPECKVMINLQRYGNTTAATIPLALTDALAEGRIKKGSLVLLASVG
                     AGFTVGSVLLRWAY"
     gene            complement(2067..>2375)
                     /locus_tag="PKK95_16355"
     CDS             complement(2067..>2375)
                     /locus_tag="PKK95_16355"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOC19843.1"
                     /translation="GPVIVAAVVFVVGFVQFGTVGMAAAASGITVVVTSLEGYLLTPW
                     LTSRAAQMNAVAIFVGLLFWAWLWGLAGVLLAVPMMLALKAISDRVEELTWLAELLGE
                     "
BASE COUNT          395 a          906 c          744 g          330 t
ORIGIN      
        1 gagaacctgc tcgtctcgat cacggccgcc cgccagcgga acgaggcgct cgaccacgtc
       61 ctgctctacg gccctccggg cctcggcaag accacgctgg cctacgtgat cggcaacgag
      121 ctgggggtgc ccgtccgctc cacctccggc ccggtgctcg agcggcccgg cgacctcgcc
      181 gcgatgctca cgaacctcaa ggagcgcgag gtcctcttca tcgacgagat tcaccggatg
      241 gcggccacca tcgaggagat cctctatccc gcgatggagg actaccagct cgacatcatg
      301 atcgggcagg gcccgagcgc ccggtcggtg aaggtgccgc tcccgcgctt cacgctgatc
      361 ggatcgacca cgcgcgccgg cctgctcacc tcgccgctgc gcggccgctt cgggatcgtc
      421 caccgcctcg acttctacac cgcctccgac atccgcgaga tcgtgatccg ctcggcgcgc
      481 atcctcggcg tcaccctgca gcccggggcg gccgaggaga ttgccggccg gtcgcgcggc
      541 accccgcggg tcgccaaccg cctgctgcgg cgggtccgcg actacgcgca ggtgcgcgcg
      601 cagggcgaga tcacccccga cgtggcccgc gcggggttga agctgctcga ggtggacgag
      661 cacggcttcg acgagatcga ccgccggctg ctgcggacga tcatcgacaa gttcggcggc
      721 ggcccggtgg ggctgaacac gatcgctgcc gccatcagcg aggagaagga cgccatcgag
      781 gacatctacg agcccttcct gattcagatc gggttcctcg atcgcacgcc ccgcggccgg
      841 gtggccacgg cccgcgcgta cgagtacttc ggcctcagga cgccggaccg cgatcaccgg
      901 ctctggtaag cgatgaagga ggagtagtgc agaaggaaag ccagaacgcc atccccgacc
      961 accggccggc cggcctgggc tgcggccgtc ccaccgagat taccgcgctc gcgacccacg
     1021 tgccgcctcg gctcctgacc aacgccgatt tcgagaagat ggtggacacc acgagcgagt
     1081 ggatcctcca gcgcaccggg atcgtcgagc gccacgtcgt cgagccgggc gttgcctcct
     1141 ccgatctcgg cgccgccgcc gccaccaagg cgattgccca ggcgggactg acccccgacg
     1201 acatcgatct catcatcgtc ggcaccacca cccccgacat gttcttcccg agcaccgccg
     1261 ccctcatcca gcacaagatc ggcgcctcgc acgcctgggg gttcgacctc gccggcgcct
     1321 gctccggctt caccttcgca ctcgccaccg gcagccagct cgtctcgacc ggccggcaca
     1381 accacgcgct gatcgtcggc gccgacgtca tgtcgtcgat cgccgattac aaggaccgcg
     1441 cgacctgcgt gctgttcggc gatggtgccg gagcggccgt cgtaagcgcc gccaaggacc
     1501 cctcgctcgc cattctcgac ttcgcccacg aggtggacgg ctccggcgct ccgttcctgc
     1561 agatgccggc cggcggaagc ctccgccccg ccacgcacga gacggtggag cagcgcctgc
     1621 actacatcaa gcaggacggc caggcggtct tcaagttcgc cgtgcgcaag accgaggaga
     1681 tcagccgcct gatcatgcag cgcaacaacc tgacgccgga cgacatcgac ctgttcgtct
     1741 ctcaccaggc caacatgcgg atcatcaagg cggccgggga gcggctcggc ttcccggagt
     1801 gcaaggtgat gatcaacctg cagcgctacg gcaacacgac cgcggccacc attcccctcg
     1861 ccctcaccga cgccctcgcc gagggccgca tcaagaaggg gagcctcgtg ctgctcgcct
     1921 cggtcggcgc agggttcacg gtcgggtcgg tgctgctccg ctgggcctac tgacagtccc
     1981 accgcagggg cgacccttgt ggccgcccct gcggcctagc aggccgtcga aaaatgcggc
     2041 ctgaatgccg cccgccgtgc gctgccctac tcacccagca gttctgccag ccaggtcagc
     2101 tcttccaccc ggtcgctgat cgccttcagg gcgagcatca tcgggacggc cagcaggacg
     2161 ccggccagcc cccacagcca cgcccagaac agcaggccca cgaagatcgc gaccgcgttc
     2221 atctgcgccg cgcggctggt cagccagggg gtcagcaggt acccctcgag cgaggtcacc
     2281 acgacggtga tccccgaggc ggctgccgcc atacccaccg tcccgaactg gacgaacccg
     2341 acgacgaaga cgacggccgc cacgatcacc ggccc
//
LOCUS       DAOPQX010000443         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_49424_length_2346_cov_4.123963, whole genome shotgun sequence.
ACCESSION   DAOPQX010000443 DAOPQX010000000
VERSION     DAOPQX010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49424_length_2346_cov_4.123963"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="PKK95_16360"
     CDS             <1..1997
                     /locus_tag="PKK95_16360"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOC19844.1"
                     /translation="ISHGGLENFAFSWSADSRWLAYSRPVESGNTALFLYDTRAGALH
                     RATLGYFSDGQPTFDPDGKYLFYASDRSFQPTYGSFDNSWTYANPTRLVALPLRRDVP
                     SPLAQRNDAEAEAGTAKEGGKGEKDEPSESGKEKAAGEKSEKGGSEKPAPRPVEIDLD
                     GLEARAVVLPPKADNYGNLHAVSGKLLFRRLAPQGAGPGAKSPILYYDLAEREEKTVL
                     DDADGFEATSDGKKLLVGSKRKFAIVEVKPAQKFEKPMRTGEMQARVDPRAEWRQIFA
                     DAYRFERDYFYDAGMHGVNWTQVRDHYLRLLDDAVTRWDVNFVLGEFIAELNASHTYH
                     GGGDLEEPARRGVGLLGVDWALDVEAGAYQVKHIVSGGPWDADVRSPLAEPGVNVKEG
                     EYVLAVNGVRLEPDRDPWSAFEGLADMPVTLTVNSRPALAGSRQVVVKTLADETELRF
                     REWIERRRKRVDEATGGKAGYIYVQSTGVDAQNELVRQFMAQWRKDGLVIDERWNSGG
                     QIPDRFIELLNRPMLSYWATRDGASQQWPPVSHVGPKVMLINGWSGSGGDAFPFYFRE
                     AKVGPLIGSRTWGGLIGISGAPPLADGGAVTVPTFRMYDVRGQWFAEGHGVEPDIAVE
                     DDPSQLAKGVDPQLERAIQELLARMAKQPSPPVRPAPEKR"
     gene            2069..>2346
                     /locus_tag="PKK95_16365"
     CDS             2069..>2346
                     /locus_tag="PKK95_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19845.1"
                     /translation="MLRSFGRVVVLTIVTAVAVGGSSTVPSAVRLPPPVVSPSAALRL
                     LPDSASGQAMPAILTLRQQAEVYNGWLKVRLEQIVPRLMREEGIDLWLV"
BASE COUNT          380 a          791 c          851 g          324 t
ORIGIN      
        1 ggatctctca tggcggcctc gagaacttcg ccttctcctg gtcagccgac tcgcgctggc
       61 tggcctacag ccggcccgtg gagagcggca acaccgcgct cttcctttac gacacgcgcg
      121 cgggggcgct gcaccgggcc acactgggct acttctccga cggccagccc accttcgacc
      181 cggacggcaa gtacctgttc tacgcgtcgg accggtcgtt ccagccgacc tacgggtcgt
      241 tcgacaactc gtggacctac gcgaacccga cgcgcctcgt cgcgctcccg ttgaggcgcg
      301 acgtcccctc gccgctcgcc cagcgcaacg acgccgaggc cgaggcgggc acggcgaagg
      361 agggcgggaa gggcgagaag gacgagcctt ctgaaagcgg caaggagaag gcggccggcg
      421 agaagagcga gaagggcggc agcgagaagc cggcgccgcg gccggtcgag atcgacctgg
      481 acggcctcga ggcgcgcgcc gtcgtgctgc cgccgaaagc cgacaattac ggcaacctgc
      541 acgccgtatc cggcaagctg ctgttccggc gcctcgcccc gcagggcgcc ggccccggcg
      601 ccaagagccc gatcctctac tacgacctgg ccgagcgcga ggagaagacg gtcctcgacg
      661 acgccgacgg gttcgaagcg acctcggacg gcaagaagct cctggtcggc agcaagcgga
      721 agttcgccat cgtcgaggtg aagccggcgc agaagttcga gaagccgatg cggaccggcg
      781 agatgcaggc gcgcgtcgac ccccgcgccg agtggcgcca gatcttcgcc gacgcctacc
      841 gcttcgagcg cgactacttc tacgacgccg gcatgcacgg ggtgaactgg acccaggtgc
      901 gcgatcacta cctgcggctc ctggacgacg cggtgaccag gtgggacgtg aacttcgtgc
      961 tgggcgagtt catcgccgag ctgaacgcct cgcacaccta ccacggcggc ggcgacctcg
     1021 aggagcccgc gcgccgcggc gtcggcctgc ttggcgtgga ctgggcgctc gacgtggagg
     1081 ccggcgccta ccaggtgaag cacatcgtgt cgggcgggcc ctgggacgcc gacgtccgct
     1141 cgccgctcgc cgagcccggc gtgaacgtga aggagggcga gtacgtcctc gccgtcaacg
     1201 gcgtgcggct cgagcccgac cgcgatccgt ggtccgcgtt cgaggggctg gcagacatgc
     1261 cggtcacgct caccgtgaac tcgagaccgg cgctcgcggg cagccggcag gtggtcgtca
     1321 agacacttgc cgacgagacc gagctgagat tccgggagtg gatcgagcgg cgccgcaagc
     1381 gggtggacga ggcgaccggc ggcaaggccg ggtacatcta cgtgcagagc accggggtgg
     1441 acgcgcagaa cgagctggtg cgccagttca tggcgcagtg gcgcaaggac ggcctggtga
     1501 tcgacgagcg gtggaacagc ggcgggcaga ttcccgaccg tttcatcgag ctgctgaacc
     1561 ggccgatgct cagctactgg gcaacgcgcg acggcgcgag ccagcagtgg ccgccggttt
     1621 cgcacgtcgg gcccaaggtg atgctgatca acggctggag cggatcaggg ggcgacgcct
     1681 tcccgttcta cttccgcgag gccaaggtgg gaccgctcat cggctcgcgc acctggggcg
     1741 gcctgatcgg catcagcggg gcgcccccgc tcgctgacgg cggcgcggtg accgtcccga
     1801 ccttcaggat gtacgacgtc cgcgggcaat ggttcgcgga ggggcacggc gtcgagcccg
     1861 atatcgcggt cgaggacgac ccgtcgcagc tcgccaaggg cgtggacccg cagctcgagc
     1921 gggccatcca ggagctgctg gcccggatgg cgaagcagcc gtcgccgccg gtccgcccgg
     1981 cgccggagaa acggtaggcg gagacagagg aggaggcggc aacaggcgtc gagcagggct
     2041 tctgccctgc tcgacaggag agactcatat gctgaggtcg ttcggtcgcg tcgtcgtgtt
     2101 gaccatcgtg actgccgtcg cggtgggtgg ttcctcgacg gtgccctccg ccgtccgcct
     2161 cccgcctcct gtcgtgtccc cgtccgccgc gctccgcctc ctgcctgaca gcgccagtgg
     2221 tcaggccatg ccggccatcc tgaccctgcg ccagcaggcg gaggtgtaca acggctggct
     2281 gaaggtgcgc ctcgagcaga tcgtgccgcg gctgatgcgc gaagagggta tcgacctgtg
     2341 gctggt
//
LOCUS       DAOPQX010000444         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_49724_length_2336_cov_3.362560, whole genome shotgun sequence.
ACCESSION   DAOPQX010000444 DAOPQX010000000
VERSION     DAOPQX010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49724_length_2336_cov_3.362560"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..350)
                     /locus_tag="PKK95_16370"
     CDS             complement(162..350)
                     /locus_tag="PKK95_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19846.1"
                     /translation="MPTDHQRRADGRELPMVERVLHFSGERGRDFDLIATHHADGEVR
                     LAIVPVPSARPDQVEAAR"
     gene            complement(340..678)
                     /locus_tag="PKK95_16375"
     CDS             complement(340..678)
                     /locus_tag="PKK95_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19847.1"
                     /translation="MSDDGYRSLGVAVLRAAFTDTRSIVPTVRASAERFFRDGDFEGW
                     AALADVDPDRVLVILERFRREMPWTRQAATDEPDEAAEVEGAAPSVNVAGAPTWRDGD
                     GENWEAENAD"
     gene            complement(668..994)
                     /locus_tag="PKK95_16380"
     CDS             complement(668..994)
                     /locus_tag="PKK95_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HOC19848.1"
                     /translation="MTIEPITRRCKVCRRPFTIEVDEVALHASLARQHPDEGWSLPQR
                     CTPCRAARRQARYAVVDDGDDEVRTCERCGRDYVFGGRDKAFWASQGWTRPRHCRPCR
                     QARHVG"
     gene            complement(1159..1830)
                     /locus_tag="PKK95_16385"
     CDS             complement(1159..1830)
                     /locus_tag="PKK95_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19849.1"
                     /translation="MKASELLKRVKAQLSQAVTTAKGQITDLRAEIGRLRRELAHAQR
                     SPLPLSDIEPRIAESVRQAGARWLAEHGEILVRSEGGIGTYQAKCGGRLPFTAAEAIP
                     FGALCAGAPDQVIELLSAIYQQVPYEAGPPAAERPALIERLKRELAEAEQVEEAAVDE
                     AAAAGVVIAHRPEVVARRRAEAAAREREEREVADRARRQEALDTEHDTRLRARFRHPE
                     PPAGL"
     assembly_gap    1993..2002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2003..2291)
                     /locus_tag="PKK95_16390"
     CDS             complement(<2003..2291)
                     /locus_tag="PKK95_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19850.1"
                     /translation="MRNPHRPASPYGRVWTWPDQEIAAASERAAAWLPPTLTIDSRSH
                     LVALWALEEPLRVGVQDVAARRLFDRVAERVGAVPLEQVARLDIVKTPGSIR"
BASE COUNT          305 a          871 c          825 g          325 t
ORIGIN      
        1 cccggcgtcg tcgtcgcgct cgagcagggc gaggatcccg ggccggcgat cgaacaggat
       61 gcgcccgtgc tcgccgagct gctcgccgtg cgcctcgagt agcgcctcga gggcgcgctc
      121 gaaacgctgc agcgcgccga tctggtcggg cagcggggcg atcatcgcgc ggcctcgacc
      181 tggtcgggac gggccgaggg gacaggaacg atggcgagcc gcacctcgcc gtccgcgtga
      241 tgcgtggcga tgaggtcgaa gtcgcgtccc cgctcgccgc tgaagtggag cacgcgctcc
      301 accatcggca gctctcggcc gtccgcgcgt cgctggtggt cagtcggcat tctccgcctc
      361 ccaattttcg ccgtcgccgt cgcgccacgt tggcgcgcct gccacgttca ccgagggggc
      421 cgccccttcc acctctgccg cctcgtcggg ctcgtctgtg gccgcctggc gcgtccacgg
      481 catctcacgg cgaaaccgct cgaggatgac gagcacgcgg tcagggtcga cgtcggccag
      541 cgccgcccat ccctcgaaat cgccatcccg aaagaaccgc tcggccgacg cgcgcacggt
      601 cggcacgatg ctgcgggtat cggtgaacgc ggcgcgcaga accgcgacac cgagcgagcg
      661 gtagccgtca tccgacatgg cgcgcctgcc ggcagggtcg acagtgccgc ggccgggtcc
      721 agccctgcga cgcccagaac gctttgtccc gcccgccgaa gacgtagtcg cgcccgcagc
      781 gctcacacgt gcggacttca tcgtcaccat cgtcgacgac ggcgtagcgc gcctgacgcc
      841 gggccgcccg gcagggcgtg cagcgctgcg gcagcgacca tccttcgtcg ggatgttggc
      901 gcgccagcga ggcgtgcagc gccacttcgt ccacctcgat cgtgaacggc cgccggcaca
      961 ccttgcagcg gcgcgtgatg ggttcgatgg tcatgatggc cccgcggccc cggagcggcg
     1021 gagtaggtga ccgccccggg gccccgccca ggagaagcga gagcaggctg ccgaccgcga
     1081 atcgcgcgag aacgcgctca cggccggctg acggtcggcc gcctgcgggc cgacgctgcg
     1141 gcgtatgccg ccgcaatctc acagacccgc gggcggctcg ggatgccgga agcgggcacg
     1201 gagccgcgtg tcgtgctccg tgtcgagcgc ctcctgacgg cgcgcacggt cggcgacttc
     1261 ccgctcttcg cgctcgcgcg ccgcggcctc ggcccgtcga cgcgcgacca cctccggccg
     1321 gtgcgcgatc accacgccgg ccgccgccgc ctcgtcaacc gccgcctctt cgacctgctc
     1381 ggcctcggcc agctcccgtt tgagtctctc gatcagcgcc ggccgctcgg ccgccggcgg
     1441 gcccgcctcg tacgggacct gctggtaaat agccgacaac agctcgatca cctggtccgg
     1501 cgcgcccgcg cacaaggcgc cgaaagggat tgcctcggcc gccgtgaacg gcaaccgccc
     1561 gccgcacttc gcttggtacg tcccgatccc cccctcgctg cgcacgagga tctcgccgtg
     1621 ctccgcgagc cagcgggcgc ctgcctgtcg gacggactcg gcgatgcgcg gctcgatgtc
     1681 gctgagcggg aggggcgacc gctgcgcgtg ggccagctcc ctgcggagcc ggccgatctc
     1741 ggcgcgcaag tcggtgatct ggcccttcgc ggttgtgacc gcctggctca gttgggcctt
     1801 gacgcgcttc aacagttcgg acgctttcat ggtcacctct tcgcgcgagc tgtcgcgcgg
     1861 gctgcaggtt cgggagtggg cgtcggcgtc ggcatcggcg ccgcggcgag ggcggcctcg
     1921 attgtggcaa ggctgtagcg gcgctcgggg gcgagctgga cgcacgtcgc cacctccgca
     1981 tcggccgagg cannnnnnnn nngccggatg ctgccgggag tctttacgat gtcgagccgc
     2041 gccacctgct cgagcggcac ggcgcccacc cgctcggcca cgcggtcgaa caagcggcga
     2101 gcggcgacgt cctgcacgcc gacgcgcagc ggttcctcca gggcccagag cgcgaccagg
     2161 tgcgagcggc tgtcgatcgt gagggtcggc ggcaaccagg ccgcggcgcg ctcgctcgcc
     2221 gcggcgatct cctgatctgg ccacgtccac acgcggccgt acgggctggc gggtctgtgc
     2281 gggttccgca tcacccgctc gaaacggaac cacgcggcga ccgccggcaa cagcaa
//
LOCUS       DAOPQX010000445         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_51058_length_2288_cov_3.399015, whole genome shotgun sequence.
ACCESSION   DAOPQX010000445 DAOPQX010000000
VERSION     DAOPQX010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51058_length_2288_cov_3.399015"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKK95_16395"
     CDS             <1..350
                     /locus_tag="PKK95_16395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC19851.1"
                     /translation="FNPIRYTSLGLAVCEAMTLGQPIVGLATTEMSVVVENGVSGFVD
                     TSVGRLIGRMRELLADRNLARRLGEGARRFARERFDIRRFARDWDAAIRDAAGRRVTA
                     VPRSLSAVTGGSA"
     gene            347..2278
                     /locus_tag="PKK95_16400"
     CDS             347..2278
                     /locus_tag="PKK95_16400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOC19852.1"
                     /translation="MTRRLAFISEHASPLALLGGVDSGGQNVYVGQLARHLACLGYEV
                     DVFTRRDAEALPEIVEWMNGVRVIHVAAGPASVLPKEELLPYMADFTAWVARFIGRER
                     RGYDLIHANFWMSALAAADIKQRLGIPFVVTFHALGRVRRFHQREADHFPDERFAIED
                     RAVAEADHVIAECPQDEEDLIRLYNADPARVTIVPCGFDSMEFWPVSKQFARIALGLA
                     PEQPVILQLGRIVPRKGIDNVIRALGRLRRDHGREVRLLIVGGATEEPDPLTMPEFAR
                     LQAIGEEEGVADLVTFVGRRKREALKYYYSAADVFVSTPWYEPFGITPVEAMACGTPV
                     VGSNVGGIKFTVRDGETGYLVPPEQPDALAERLAHVYAHPRLLSLLRRQAIRRVNDLF
                     TWQRVATSASELYESVIAAQQPPSADDADGLAAVDRRFDAVVAALHETRRRLRSAIVE
                     AARVIADGFARDGKALVGAVNGSGPEARRVAADLMARSRSADRPGLPALALTTDDLPA
                     WGGDAPAEALLARQVEAFGRAGDVLIAVSAGAPTRDFTHSLEVARGRGLRSVAVLGAE
                     IGDARHLADVAIVAPVSEPEQVREVQQVMLRLLCDLVEQRLSRRPPLAGRGLPHDAAT
                     PGRTRRAVGGRRRTGDDAA"
BASE COUNT          312 a          805 c          842 g          329 t
ORIGIN      
        1 tcttcaaccc catccgttat acgagcttgg gcttggcggt gtgcgaggcg atgacgctcg
       61 gacagccgat tgtcggcctc gccaccaccg agatgtcggt tgtcgtcgag aacggcgtgt
      121 cgggtttcgt ggacaccagc gtggggcgcc tgatcgggag gatgcgcgag ctgctggcag
      181 accggaatct tgcacgccgc ctgggcgagg gcgcccggcg cttcgcccgc gagcggttcg
      241 acatccggcg cttcgcgcgc gactgggacg cggccatccg cgacgccgcc ggccgccgcg
      301 tgacagcggt gcccaggtcg ctttccgccg ttaccggggg gagcgcatga ccagacggct
      361 cgccttcatc agcgagcacg cctcgccgct ggcgctgctc ggaggcgtgg acagcggagg
      421 gcagaacgtc tacgtgggcc agctcgcgcg ccatctcgcc tgcctcggct acgaggtgga
      481 cgtgttcacc cgccgggacg cggaggcgct gccggagatc gtcgagtgga tgaacggcgt
      541 gcgcgtgatt cacgtggccg caggccccgc ttcggtgctg ccgaaggagg agctgctgcc
      601 gtacatggcc gacttcaccg cgtgggtcgc ccgcttcatc gggcgcgagc ggcgcggcta
      661 cgacctgatc cacgcgaatt tctggatgtc ggcgctggcg gcggccgaca tcaagcagcg
      721 gctgggcatc cccttcgtcg tgaccttcca tgccctcggg cgcgtgcgcc gcttccacca
      781 gcgcgaggcc gaccacttcc ccgacgagcg gttcgccatc gaggaccgcg ccgttgccga
      841 ggccgaccac gtgattgccg agtgcccgca ggacgaggag gacctgatcc gcctctacaa
      901 cgcagaccct gcccgcgtga cgatcgtgcc ctgcgggttc gactcgatgg agttctggcc
      961 ggtcagcaag cagttcgcgc ggatcgccct cggcctcgcg cccgagcagc cggtgatcct
     1021 ccaactcggg cgcatcgtcc cccgcaaggg gattgacaac gtcatccgcg cgctcggccg
     1081 cctgaggcgc gaccacggcc gcgaggtgcg gctgctgatc gtgggaggag ccaccgagga
     1141 acccgacccg ctgacgatgc ccgagttcgc gagactgcag gcgattggcg aggaagaggg
     1201 cgtggccgac ctggtgacct tcgtcggccg gcgcaagcga gaggcgctca agtactacta
     1261 cagcgcggcg gacgtcttcg tcagcacgcc gtggtacgag ccgttcggca tcacgccggt
     1321 cgaggcgatg gcctgcggca caccggtcgt cggctccaac gtcggcggca tcaagttcac
     1381 cgtgcgggac ggcgagaccg gctacctggt gccgcccgag cagcccgacg cgctggccga
     1441 gcggctcgcg cacgtctacg cgcacccccg gctgttgtcg ctgctgcggc gccaggcgat
     1501 tcgccgggtc aacgacctct tcacctggca gcgggttgcc acgtctgcct cggaactcta
     1561 cgagagcgtg atcgcggcgc agcagccccc cagcgcagac gacgccgacg gccttgccgc
     1621 ggtggaccgg cgcttcgacg ccgtcgtggc ggcgcttcac gagacgcggc gccggctgcg
     1681 gtctgccatc gtcgaggcgg cccgggtgat cgccgacggg ttcgcgcggg acggcaaggc
     1741 gctggttggc gcggtcaacg gcagcggtcc ggaggcgcgg cgcgtggcgg ccgatctgat
     1801 ggcgcgctcc cgcagcgccg accgccccgg cctgccagcc ctggcgctca ccaccgacga
     1861 ccttccagcg tggggaggcg acgccccggc cgaggcactg ctcgctcggc aggtggaggc
     1921 cttcggccgc gcgggcgacg tgctgatcgc ggtgtcggcc ggcgcgccga cacgcgactt
     1981 cacccactcg ctggaggtgg cgagggggcg aggtcttcga tcggtcgccg tgctcggcgc
     2041 cgagatcggc gacgcgcggc acctcgccga cgtggcgatc gtcgcaccgg tttcggaacc
     2101 cgagcaggtg cgcgaggtcc agcaggtgat gctgcgcctc ctctgcgacc tcgtcgagca
     2161 gcggctgtct cgccggccgc cgctcgccgg gcggggcctt ccgcacgacg cagcgacgcc
     2221 cgggaggacg cgacgggccg tcgggggccg ccgaaggacg ggagacgacg ccgcgtgagc
     2281 cgcgagga
//
LOCUS       DAOPQX010000446         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_52131_length_2252_cov_4.142922, whole genome shotgun sequence.
ACCESSION   DAOPQX010000446 DAOPQX010000000
VERSION     DAOPQX010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52131_length_2252_cov_4.142922"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1066
                     /locus_tag="PKK95_16405"
     CDS             137..1066
                     /locus_tag="PKK95_16405"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOC19853.1"
                     /translation="MIPRPLTGIVPPLVTPMRGRDELDVPGLERLIEHVLDGGVSGLF
                     VLGTTGEGPSLGYRLRRELVERTCRQVRGRVPVLVGITDTAFVESLALARFAADAGAD
                     ALVAAPPYYLPGGQPELREYLRHLVRELPLPLFLYNMPSLAKVSFEIETVRYAMDDPR
                     VAGLKDSSGDMAYFRDVAALLARRPGWSLLVGPEERLLEAIEAGGQGGVNGGANLFPA
                     LYVGLYEAFRAGDTARARRLHAQVVRVSESLYRIGRHPSAVIKGIKCALACLGICDDF
                     MAEPFHRFRARERAVVEQRLAALQDEIAGALGR"
     gene            complement(1158..1445)
                     /locus_tag="PKK95_16410"
     CDS             complement(1158..1445)
                     /locus_tag="PKK95_16410"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOC19854.1"
                     /translation="MHSSSIVELPDGRLFAVYYRGSGERRADDVRLEGALLARGGREW
                     SSPFQVAETPGFPDCNPVTFLDGLGRLVVAWRPSRTRGRACRCWRFAAARG"
     gene            complement(1464..1886)
                     /locus_tag="PKK95_16415"
     CDS             complement(1464..1886)
                     /locus_tag="PKK95_16415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOC19855.1"
                     /translation="MPWIQKSFALSPRPRGFHLVTDEVARQLPELQTIERGLLHLFIQ
                     HTSASLSIGENADPSVRADFESHFDVLAPENARHYTHDAEGPDDMPAHLKSALLGCSV
                     TIPITAGRLNLGTWQGIYLGEHRDRGGSRRVVATVMGE"
BASE COUNT          330 a          806 c          796 g          320 t
ORIGIN      
        1 ggaggaggga ggccgacggc acctggaaga tcgtcctcga caacggctgc ccgccctgcg
       61 aaggccgttg acacgacaca ggaagcaggg ctgaagccct gctctacgta agcgcgctcc
      121 accggtgcct tcaacgatga tcccacgacc tctcaccggc atcgtcccgc cgttggtcac
      181 gccgatgcgc ggccgcgacg agctggacgt gcccggcctc gagcggctga tcgagcacgt
      241 gctggacggc ggcgtgagcg ggctgttcgt cctgggcacc accggcgagg gaccgagcct
      301 cggctaccgc ctgcgccgcg agctggtgga gcgcacctgc cggcaggtgc gaggccgcgt
      361 gccggtgctc gtcggcatca ccgacacggc gttcgtcgaa tcgctggccc tggcccgctt
      421 cgcggccgac gcgggcgccg acgccctggt ggccgctccg ccttactacc tgccgggcgg
      481 ccagcccgag ctgcgcgagt acctccgcca cctcgtgcgc gagctgccgc tgccgctgtt
      541 cctctacaac atgccgtcgc tcgccaaggt ctccttcgag atcgagaccg tccgctacgc
      601 gatggacgac ccgcgcgtcg ccggcctcaa ggacagctcg ggcgacatgg cctacttccg
      661 ggacgtggcg gcgctgctcg cgcggcgccc cggctggtcg ctgctcgtcg ggcccgagga
      721 gcggctgctg gaggcgatcg aggcgggagg acagggcggc gtcaacggag gggccaacct
      781 gtttcccgcg ctgtacgtcg gcctgtatga agccttccgc gccggcgaca cggcgcgcgc
      841 acgacggctg catgcccagg tggtgcgggt cagcgagtcg ctgtaccgca tcggccggca
      901 cccctcagcg gtgatcaagg gaatcaagtg cgcgctcgcc tgcctcggca tctgcgacga
      961 cttcatggcc gagccgttcc accgcttccg cgcgcgcgag cgggcggtgg tggagcagcg
     1021 cctggccgcg ctgcaagacg agatcgcagg ggcgctggga aggtagatcg gccgccggaa
     1081 gggccacgat cgcccttcgt cgcgagacag ccaggccgag aggcgcgcgc gcccgcgctc
     1141 ggtgtcgttc gaaaccatca gccacgagcc gccgcgaagc gccagcacct gcaggctcga
     1201 ccccgggttc gggatggacg ccaggcgacc acgaggcggc ccaggccgtc gaggaacgtg
     1261 accgggttgc agtcggggaa ccctggggtc tcggcaacct gaaacgggct gctccactca
     1321 cggccgccgc gcgccagcag cgccccctcc agccgcacat cgtcggccct gcgctcgccg
     1381 ctgccacggt agtagacggc gaacaaccgg ccgtcgggca gctccacgat ggacgaggag
     1441 tgcacgtgct cgccctgcgt cccctactcc cccatcaccg tcgccaccac cctgcgcgag
     1501 ccgccgcggt cacggtgctc gcccaggtag atgccctgcc aggtgccgag gttcaggcgg
     1561 cccgccgtga tcgggatcgt gacgctgcag ccgagcagcg ccgacttgag gtgcgccggc
     1621 atgtcgtcgg gcccctcggc gtcgtgggtg tagtgccggg cgttctcggg ggccagcacg
     1681 tcgaagtgcg attcgaagtc ggcgcggacg ctcgggtcgg cgttctcgcc gatcgacagc
     1741 gacgcggagg tgtgctggat gaagaggtga agcaggcccc gctcgatcgt ctgcagctcg
     1801 gggagctgcc gggcgacctc gtcggtgacg aggtggaagc cgcggggccg cggcgacagc
     1861 gcgaacgact tctggatcca gggcacgtgg cctcctcgaa acggaaatgg ggctagctta
     1921 gcgcaggtca caggcgagac gcctgtgcca ctggctcaat ggcggccatc acaggcgaga
     1981 cgcctgtgcc acttcccaca ggcgagacgc ctgtgccact cctcacaggc gagacgcctg
     2041 tgccactggc tctgtgccac cccggactcg cggcgctggt cttgcaccgg gcgcccggcg
     2101 tccacccgcc aactggaggc gccccatgac agacagcccc gaacggcgct cgaccgttcg
     2161 ttcgaagtgg cagattcttg cggtgacgtt catcctggcg ctgccggcaa tctccgcggc
     2221 gcaggccgcc cctgccgtcg aggtgtccgc cg
//
LOCUS       DAOPQX010000447         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_54765_length_2170_cov_5.132388, whole genome shotgun sequence.
ACCESSION   DAOPQX010000447 DAOPQX010000000
VERSION     DAOPQX010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54765_length_2170_cov_5.132388"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PKK95_16420"
     CDS             <1..556
                     /locus_tag="PKK95_16420"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HOC19856.1"
                     /translation="NGLTDYSHPCQAMADYLTIREVKGRTEGIKVVFVGDGNNVAHSL
                     MFAGALLGSHVTVVTPPGYEPKADAVAWATAQSARTGGSCTVTNELSAGVDAADVIYT
                     DVWASMGQEAEAESRKQIFLRYQVNEAVFERAKPDAVFMHCLPAHRGEEVTAGVIDSP
                     RSVVFQEAENRLHAQKAIMLELMT"
     gene            586..1584
                     /locus_tag="PKK95_16425"
     CDS             586..1584
                     /locus_tag="PKK95_16425"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOC19857.1"
                     /translation="MPRSALVAIGGNSLIRAGEKGTIAEQLANTRRTAEAIAGLVGDG
                     YSLVLTHGNGPQVGAALLRSERASDQVYSQSLDVCDAATEGEIGYLLQQSLHNALAAA
                     GLDRPVATVLTQVLVSTDDPAMRNPTKPIGPFYSRADAEERARVLGWTIVEDAWRGYR
                     RVVPSPEPLELVEEGVVAGLLERGVLVIAAGGGGIPVVRTPEGLRGVEAVIDKDRVSA
                     LLAARLRVDTFIISTDADRVYLDYRRPTQRPLHRVTAHQMRRHLEAGQFPPGNMGPKV
                     ESALRFLDAGGREVIITSYEHLLEAVRGQAGTHITRAEGEGREAVNGSAKAEGGGR"
     gene            1581..>2170
                     /locus_tag="PKK95_16430"
     CDS             1581..>2170
                     /locus_tag="PKK95_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19858.1"
                     /translation="MILSHVVESQQFTVPLLMELFDRTRRMERVAARGGTLDYQGRIM
                     ASLFYRPSTRTRFSFEAAMYRLGGKVLSTEQAGYFSSEVEGEQLEDTIRILGGYADVI
                     VIRHTQAGGARRAAEVSPVPVINAGDGNGGQHPTQALLDLYTIYRERPIEGLSIAFIG
                     ELDRGRTARSLAYLLAKFERVKIYFVSPPEMQMGLD"
BASE COUNT          342 a          759 c          770 g          299 t
ORIGIN      
        1 caacgggctc accgattaca gtcacccctg ccaggccatg gccgactacc tgaccatccg
       61 cgaggtgaag gggcgcaccg agggcatcaa ggtcgtcttc gtcggcgacg ggaacaacgt
      121 cgcccactcc ctcatgttcg ccggggcgct cctcgggtcg cacgtcacgg tggtcacgcc
      181 gcccgggtac gagccgaagg ccgacgccgt cgcatgggcg acggcgcagt ccgcccgcac
      241 cggcggatcg tgcacggtca ccaacgaact ttcggccggc gtcgacgccg ccgacgtgat
      301 ctacaccgac gtctgggcga gcatgggcca ggaggcggag gccgagtcgc gcaagcaaat
      361 cttccttcgg taccaggtca acgaggcggt gttcgagcgc gcgaagcccg acgccgtctt
      421 catgcactgc ctcccggccc accggggcga ggaggtcacc gccggcgtca tcgactcgcc
      481 ccgctcggtc gtcttccagg aggccgagaa ccgcctccac gcgcagaagg cgatcatgct
      541 ggagctcatg acgtaggggc tgggagacta ccccgaatcc tgaatatgcc gcgcagcgca
      601 ctggtcgcca tcggcggcaa ctccctgatt cgcgcgggtg agaaggggac catcgccgag
      661 cagctcgcca acacacggcg gacggccgag gcgatcgccg gcctggttgg cgacggctac
      721 agcctcgtcc tcacgcacgg caacggcccg caggtaggcg ccgcgctgct tcgctcggag
      781 cgcgcctccg accaggtgta cagccagtcg ctcgacgtct gcgacgccgc caccgagggg
      841 gagatcggct acctgctgca gcagtccctc cacaacgcgc tcgcggccgc cgggctcgac
      901 cggccggtgg cgacggtgct gacccaggtg ctggtctcga ccgacgaccc ggcgatgcga
      961 aaccccacca agccgatcgg tcccttctac tccagggccg acgccgagga gcgcgcccgc
     1021 gtgctcgggt ggacgatcgt cgaggatgcc tggcgcggct accggcgcgt cgtcccctcg
     1081 cccgaaccgc tggagctggt cgaggagggg gtggtcgccg ggctgctcga gcgcggggtc
     1141 ctggtgatcg cggcgggcgg gggaggaatc ccggtcgtgc ggacaccgga ggggctgcgg
     1201 ggcgtggaag cggtgatcga caaggaccgg gtctcggcgc tgctcgcggc ccggctgcgc
     1261 gtcgacacct tcatcatctc caccgacgcc gaccgtgtgt acctggacta ccgccggccg
     1321 acgcagcgcc cccttcaccg ggtgacggcc caccagatgc gccgccacct cgaggcgggc
     1381 cagttccctc cgggcaacat gggcccgaag gtcgagtcgg cgctgcggtt cctcgacgcc
     1441 ggcggccgcg aggtgatcat cacttcctac gagcacctgc tcgaggccgt gcgcggccag
     1501 gccggcacac acatcacgag ggcggagggc gaagggcggg aggcggtgaa cggcagcgcg
     1561 aaggcggaag gcggaggccg atgatcctgt cgcacgtcgt cgaatcgcag cagttcaccg
     1621 tgccgctgct gatggagctg ttcgaccgga cgcgccggat ggagcgggtg gcggcccgcg
     1681 ggggcacgct cgactaccag ggccggatca tggcctccct cttctaccgg ccctcgaccc
     1741 ggactcggtt ttccttcgag gcggcgatgt accgcctcgg cggcaaggtg ctctcgaccg
     1801 agcaagcggg ctacttctcc tcggaggtcg agggcgagca gctcgaggac acgatccgca
     1861 tcctcggcgg gtacgccgac gtgatcgtca tcaggcacac gcaggccgga ggggcgcggc
     1921 gcgcggccga ggtttcgccg gtcccggtga tcaacgcggg agacggcaac ggggggcagc
     1981 acccgacgca ggccctgctc gacctgtaca cgatctaccg ggagcggccg atcgaggggc
     2041 tgagcatcgc gttcatcggc gagctcgacc ggggccggac cgcgcgctcc ctcgcctacc
     2101 tgctggcgaa gttcgagcgg gtgaagatct acttcgtcag cccccccgag atgcagatgg
     2161 gcctcgacat
//
LOCUS       DAOPQX010000448         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_56362_length_2125_cov_4.250242, whole genome shotgun sequence.
ACCESSION   DAOPQX010000448 DAOPQX010000000
VERSION     DAOPQX010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56362_length_2125_cov_4.250242"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..862
                     /locus_tag="PKK95_16435"
     CDS             104..862
                     /locus_tag="PKK95_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOC19859.1"
                     /translation="MATVASSPVISAAIRTLCIIDVPRGESGPESPGLQANTPYSSVR
                     REQPTPSRALARQALLDASRNELYTSGERLFVICEGGEQRMPRAHETRVLLLVFSLAV
                     SLACIGGVAQAPHGGGSGRGAEPSSSLEAEQELFDRVNAYRVSKGLTALRWSDVVAEQ
                     ARGHSRDMASGAAPRGHAGIEARVAEIRKHIPIGAWAENVVGERNADEAFRRLVDSRT
                     HRRNLERDFDLTGVGAATDSEGLLYLTQIFVKSK"
     gene            complement(1147..2085)
                     /locus_tag="PKK95_16440"
     CDS             complement(1147..2085)
                     /locus_tag="PKK95_16440"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOC19860.1"
                     /translation="MRSTSMLVATGILMSYPCLASGEIHKHAGSPAALPHDKYVRVVS
                     EADSPIQQAYVKSKDGAYVAAAIRKPKGEGPFPAIIMFHGAPGGRGMEQLVGWSRGDH
                     GGPVWERFLQEGFVVVVGDYRGGDWNAMNVPSTGAGATAIDDGLAIIQHVERLPYVDA
                     KRISLYGVSLGGNLVAFLVSKVPAIHSAILGAPAPIWFLGVQMPPGGRRDYSNPKFDP
                     AVAAANIAPIRTPLLILAGTADGLLPMATLLHDQLAQAGKTVRMDVYEHGYHDFVLGP
                     QGQKRPDLPQGEVILQGTLEALELSVSFVKAPASSH"
BASE COUNT          327 a          729 c          726 g          343 t
ORIGIN      
        1 cgcggcccac ggcactccca cgtgcctttg aatcgctgcg acgacttctc gagcggtcgg
       61 cttcgtcatc tcgaccgatt gcctctcctg ggcgccggcg ctcgtggcca cggttgcgag
      121 cagtcctgtc atcagtgccg ccatccgcac gttgtgcatc atcgatgtgc ctcgcggtga
      181 atcgggtccc gaatctcccg gccttcaggc gaatacgccg tattctagcg tccggcgcga
      241 acagccgacg ccatcccgcg cgctggcgcg ccaggcgctg ctggatgcgt cgcgaaacga
      301 gttgtacact tctggcgagc gcctcttcgt catctgcgaa ggtggagagc agcgaatgcc
      361 gcgtgctcat gaaacgagag tactgctgct cgtcttctcg ctggccgttt cgctcgcctg
      421 tatcggcggc gtcgcgcagg caccgcatgg cggcggttcc ggccggggtg ccgagccgtc
      481 atcgagcttg gaggccgagc aggagttgtt cgaccgcgtc aacgcctacc gcgtgtcgaa
      541 agggctgacg gcccttcggt ggagcgacgt cgtcgcggaa caagcgcgtg gccacagccg
      601 cgacatggcc tcaggcgcgg cgccgcgtgg acatgcgggc atcgaggcgc gcgtcgcgga
      661 gatcaggaag cacatcccca tcggcgcctg ggccgagaac gtggtcggcg aacggaacgc
      721 agacgaggcc ttccgccggc tggtcgacag ccgcacgcac cgcaggaacc tggagcgtga
      781 cttcgatctc accggggtgg gcgcggcaac cgactcggaa ggcctgctgt acctcacgca
      841 gatcttcgtg aagtcgaagt agcgggacgc ggagggctga aaacgcgaag ggccaccgct
      901 tggtggcccc tcttctacct gccgagtggg gtgtgcgccc ctatccgtgt cgctgtgcca
      961 cgcgcgcagg ccaatcggga cgatagcaat cgacgctcca caccgccagc ccggcagcgc
     1021 cgcgcgtctc gggggcggca agcctctttc ccgtaatcga ttggaacgat ccgccgttcc
     1081 gccggggcgg gtcggcagcg gcgcggagtc gcaccccgcg gagactaatg tgggcagcgt
     1141 gccggctcag tgggacgagg ccggcgcctt cacgaacgac accgacagtt caagcgcctc
     1201 cagcgtcccc tggaggatca cctcgccctg cggcaggtcg ggccgtttct gtccctgcgg
     1261 gccgagcacg aagtcgtggt agccgtgctc gtagacgtcc atccgcaccg tcttccccgc
     1321 ctgcgcgagc tggtcgtgaa gcagggtcgc catcggcagc agcccgtcgg ccgtgccggc
     1381 caggatcaga agcggggtgc ggatgggcgc gatgttggcg gcagcgaccg ccgggtcgaa
     1441 cttcgggttc gagtaatccc ggcgcccacc cggcggcatc tgcacgccga ggaaccagat
     1501 cggcgccggc gcccccagga tcgccgagtg aatcgccggc accttcgaaa cgaggaacgc
     1561 gacgaggttg cccccgaggc tcacgccgta gaggctgatc cgcttggcgt ccacgtacgg
     1621 caggcgctcg acgtgctgga tgatcgccag cccatcgtct atcgcggtgg ctcccgcgcc
     1681 cgtcgagggg acgttcatcg cgttccagtc tccaccgcgg tagtcgccga ccaccaccac
     1741 gaagccctcc tggagaaatc tctcccagac cgggccgccg tgatcgccgc gcgaccagcc
     1801 caccagctgc tccatgccgc gtccacccgg ggcgccgtgg aacatgatga ttgccgggaa
     1861 gggcccctcg cccttcggct tgcggatggc ggccgcgacg taggcgccgt ccttcgactt
     1921 gacgtacgcc tgctgaatgg gcgagtcggc ctcgctgacc accctgacgt acttgtcgtg
     1981 cggcagcgcg gcggggctgc cggcgtgctt gtggatctcg ccggaggcga ggcaggggta
     2041 tgacatgagg atgccggtcg ccacgagcat ggacgttgag cgcatatgga ccggcattct
     2101 accccgcttc gccgcgcgcg cgcgc
//
LOCUS       DAOPQX010000449         2079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_58086_length_2079_cov_3.426877, whole genome shotgun sequence.
ACCESSION   DAOPQX010000449 DAOPQX010000000
VERSION     DAOPQX010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58086_length_2079_cov_3.426877"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PKK95_16445"
     CDS             complement(<1..667)
                     /locus_tag="PKK95_16445"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOC19861.1"
                     /translation="MSTIICSGACAKAVALALALIVVAAAAGLQGASPQSGQAPAAVA
                     VERASLIPLPASVSMTPGESFAVTKETVIEVSPKQPEVRRIAEMLAALVWPALEARLA
                     IRDAGPAPLPGTIRLEVDPSGSRLEEGYELAVAREGIRLAAKTPAGLFYGFQTLRQLM
                     PWTVELRGIRPFALTVPAAVITDAPRFGWRGAMLDVARHFFTPAEVKRYIDLLALYKI
                     NRLH"
     gene            811..1926
                     /locus_tag="PKK95_16450"
     CDS             811..1926
                     /locus_tag="PKK95_16450"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOC19862.1"
                     /translation="MTTILLALLSFALQSAPAAKPPERFAATQPLAELQNKQAVLETD
                     AGTIVIDLLPDAAPNHVAYFLKLAAEGAYNGTTFHRVIKYGIIQGGDPLSKDPAKRPA
                     YGTGGLNVLAAEPGAGKHTAGTVSSVILPGRPDSGGAQFFICVVDQPALDGQYTAFGR
                     VAEGMSVVRRISEAGVDENGRVVERIEIKSAVIRERPPEPPTFADEPAEQLAAYRAVV
                     ETSAGRITLAFRPDKAPGHVRNFLSLAAAGVYDGTTVHRVVRGFVIQGGSMNFRREPL
                     NESQQRRVRNLEPEFNDLPHRLGTLSMARGDDPASASTSFFICTGQASSLDGKYTAFG
                     EVVDGLSVVQAIEGAPVDGEAPRERIEIIKITVVKST"
BASE COUNT          326 a          700 c          743 g          310 t
ORIGIN      
        1 ggtggaggcg gttgatcttg tagagcgcca gcaggtcgat gtagcgcttc acctcggcgg
       61 gggtgaagaa gtggcgcgca acgtcgagca tcgccccgcg ccagccgaag cgcggcgcgt
      121 cggtaatgac ggcggccggc acggtgaggg cgaaggggcg gatgccccgc agctcgaccg
      181 tccagggcat cagctggcgc agggtctgga acccgtagaa cagcccggcg ggcgttttgg
      241 cggcgaggcg gatgccctcg cgggcgaccg cgagctcgta gccttcctcg aggcgcgagc
      301 ccgacggatc gacctcgagg cgaatcgtgc cggggagtgg cgccgggccc gcgtcgcgga
      361 ttgcgaggcg ggcctcgagc gccggccaca ccagggcggc cagcatctcc gcgatgcggc
      421 gcacctcggg ttgcttcggc gagacctcga tcacggtctc cttggtgacg gcgaacgact
      481 cgccgggcgt catcgacacc gacgcgggca gcgggatcag ggaggcgcgc tcgacggcaa
      541 ccgccgccgg cgcctggccc gactggggcg aggcgccctg caggccggcg gccgcggcga
      601 cgacgatgag cgcgagggcc agcgcgacag ccttggcgca tgcgccggag cagatgatgg
      661 tgctcatgac gccgattcta cgccctggac tcgacttcca ggctctttga cttcgcgact
      721 cgacctcgtg acttcgcgac ttcgcgacct cgtgacttca cgacttcgcg acctcgtgac
      781 ttcgtggact gcggagctta gtatcacctc gtgaccacca tcctcctcgc cctgctgtct
      841 tttgctctcc agtcggcccc ggcggcgaag ccgcccgagc gcttcgccgc cacgcagccg
      901 ctcgccgagc tgcagaacaa gcaggccgtg ctcgagacgg atgccgggac catcgtcatc
      961 gacctgctgc ccgatgcggc gcccaatcac gtggcctact tcctgaagct cgcggccgag
     1021 ggggcgtaca acgggaccac gtttcaccgg gtcatcaagt acggcatcat ccagggcggc
     1081 gacccgctgt cgaaggaccc ggccaagcgg ccggcgtacg gaacgggagg cctgaacgtg
     1141 ctggcagccg agcctggggc cgggaagcac accgcgggca ccgtgtcgtc ggtcatcctc
     1201 ccgggacggc ccgactccgg tggcgcgcag ttcttcatct gcgtcgttga ccagccggct
     1261 ctggacgggc agtacacggc cttcggacgc gtggccgagg gcatgtccgt ggttcgccgc
     1321 atctcggagg cgggggtcga cgagaacggc cgggttgtgg agcgcatcga gatcaagtcg
     1381 gccgtcatcc gcgagcggcc gccagagccg cccaccttcg ccgacgagcc agccgagcag
     1441 ctcgccgcct accgggcggt ggtcgagacc tccgccggca ggatcacgct cgcatttcgc
     1501 cccgataagg ctccggggca cgtccgaaac ttcctgagcc tcgccgcggc cggtgtctac
     1561 gacggcacga cggtccaccg cgtcgtgcgg gggttcgtca tccagggcgg ctcgatgaac
     1621 ttccggcgcg agccgctgaa cgagtcgcaa cagcggcggg tgcgcaacct ggagccagag
     1681 ttcaacgacc ttccgcaccg cctgggcacc ctgtcgatgg cgcgcggcga cgacccggcc
     1741 agcgcatcca cctcgttctt catctgcacc ggccaggctt cctcgctcga cggcaagtac
     1801 acggccttcg gcgaggtggt cgatggcctt tccgtcgtgc aggcgatcga gggggcgccc
     1861 gtagacggcg aggcgccccg cgagcgaatt gagatcatca agatcacagt cgtgaagtcc
     1921 acgtagtcgc gaagtcacga agtcgcgaag tcggagtcca cggactctcg tcttggagtt
     1981 ggcgcgagcc tcggcgattg cgaggaggga agaggtgagc gagcgcgacg gcctggtgac
     2041 cggggaggat ggccggaacc gctgctggtg gtgcgtgcc
//
LOCUS       DAOPQX010000450         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_59653_length_2038_cov_3.931921, whole genome shotgun sequence.
ACCESSION   DAOPQX010000450 DAOPQX010000000
VERSION     DAOPQX010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59653_length_2038_cov_3.931921"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKK95_16455"
     CDS             <1..622
                     /locus_tag="PKK95_16455"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOC19863.1"
                     /translation="VLILGDGLIGQIMEPIEFDPDRKPCKALPPKTWATTGCAGDRPP
                     NIITSLFLDPEPLAAHNEKLQAKYAAMARDEVRVSGRGLDEPYDVLVVAYGTVARACN
                     MAITQLRESGVRAALLRPVSLFPFPAAAVRAAAARADAVLVVELSAGQMVEDVRAAVG
                     DTCPVHFHGRTGGLMIPAEEVAEKVRALATGARLPSRAAEREVVDA"
     gene            615..1409
                     /locus_tag="PKK95_16460"
     CDS             615..1409
                     /locus_tag="PKK95_16460"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOC19864.1"
                     /translation="MPDTIFARSPVLTSTSTHYCPGCTHGVIHRMVAEVVDELGIRAR
                     TVGIAPVGCAVLAYNYFGLDFQEASHGRAPAVATGLKRARPDLVVFAYQGDGDLASIG
                     TAEIVHCANRGEKITVVFVNNAIYGMTGGQMAPTTLPGQVTTTCAFGRDVAHAGFPIR
                     VAELLSTLRTPSFIARGAVHTPREAARAKALLRRAFQNQIDNACFSLVELLSTCPTNW
                     GLSPAEAGQWLERNMVPYYPLGVFRTPEGGDRAPLPVAGEQAWNTR"
     gene            1394..1951
                     /locus_tag="PKK95_16465"
     CDS             1394..1951
                     /locus_tag="PKK95_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOC19865.1"
                     /translation="MEHQVIIAGFGGQGVMLIGKILAHAGMKEGKEVSWLPSYRPEMR
                     GGTSNCTVVVSDEPVGSPLVYRPSAVIAMNEPSLAKFEPRVAPGGVLLVNTCLAHGAR
                     SRADVRVVPIAANDIAAAVGNARAANMAALGAYLGSTGSVSLETAETVIREMLAGKPQ
                     VVESNLEALRRGHALGVKALQQEAA"
BASE COUNT          281 a          758 c          720 g          279 t
ORIGIN      
        1 ggtcctgatc ctcggcgacg gcctgatcgg ccagatcatg gagccgatcg agttcgaccc
       61 cgaccggaag ccctgcaagg cgctgccgcc caagacctgg gccacgaccg ggtgcgcggg
      121 cgaccgtccg cccaacatca tcacctcgct gttcctcgac cccgagccgc tcgcggccca
      181 caacgagaag ctgcaggcga agtacgccgc gatggcgcgc gacgaggtgc gcgtctcggg
      241 ccgcggcctc gacgagccgt acgacgtgct cgtggtcgcg tacggaaccg tcgcgcgggc
      301 ctgcaacatg gccatcaccc agctgcgcga gtcgggcgtc cgcgccgcgc tgctccggcc
      361 cgtcagcctc ttccccttcc cggccgccgc cgtccgcgcc gccgccgcgc gcgccgacgc
      421 ggtgctcgtc gtcgagctgt cggccgggca gatggtcgag gacgtgcgcg ccgcggtcgg
      481 cgacacgtgc cccgtccact tccacggccg cacgggagga ctcatgatcc ccgccgagga
      541 agtggcggag aaggtcaggg ctctcgccac cggggcgcgg ctgccgtcgc gggccgccga
      601 gcgcgaggtg gtcgatgcct gacacgatct tcgcccgctc tccggtcctg acctcgacct
      661 ccacccacta ctgcccgggg tgcacgcacg gggtgatcca ccggatggtg gccgaggtgg
      721 tggacgagct gggaatccgc gcccggacgg tcggcatcgc gccggtcggg tgcgcggtgc
      781 tcgcctacaa ctacttcggc ctcgacttcc aggaagcctc gcacggccgc gccccggcgg
      841 tcgccacggg cctcaagcgc gcccgccccg atctcgtcgt gttcgcctac cagggcgacg
      901 gcgacctcgc ctcgatcggc accgccgaga tcgtccactg cgccaaccgc ggcgagaaga
      961 tcaccgtcgt cttcgtcaac aacgcgatct acgggatgac cggcggccag atggccccga
     1021 cgacgctgcc cggccaggtc acgaccacgt gcgccttcgg ccgggacgtc gcgcatgccg
     1081 ggttcccgat ccgcgtcgcc gagctgctga gcacgctgcg cacgccgtcg ttcatcgccc
     1141 ggggcgccgt gcacacgccg cgcgaggcgg cccgggccaa ggcgctgctg cgccgagcct
     1201 tccagaacca gatcgacaac gcctgcttct ccctcgtcga gctcctctcg acgtgcccca
     1261 ccaactgggg gctgtcgccg gcggaagcag gccaatggct ggagcggaac atggtgccct
     1321 actacccgct cggcgtcttc aggacgccgg agggcgggga ccgcgctccg ctgccggtcg
     1381 ccggggagca ggcatggaac accaggtgat catcgcgggg ttcggtggcc agggggtcat
     1441 gctcatcggg aagatcctcg cccacgccgg catgaaggag ggcaaggagg tgtcgtggct
     1501 gccgtcctac aggcccgaga tgcggggcgg cacctcgaac tgcacggtgg tcgtctcgga
     1561 cgagccggtc ggctcgccgc tcgtctaccg gccgtcggcc gtcatcgcga tgaacgagcc
     1621 gtcgctggcg aagttcgagc cgcgcgtggc gccgggcggc gtgctgctgg tgaacacctg
     1681 cctcgcgcac ggcgcccgct cccgggccga cgtgcgcgtg gtgccgatcg ccgccaacga
     1741 catcgcggcc gcggtgggca acgcgcgcgc ggcgaacatg gccgccctgg gcgcgtacct
     1801 cggctccacc ggttcggtgt cgctcgagac ggccgaaacg gtcatccgcg agatgctcgc
     1861 gggcaagccg caggtggtcg agtcgaacct cgaggcgctg cggcgcggtc acgccctcgg
     1921 ggtcaaggcg ctgcagcagg aggcggcgtg acgctcgcct gccccgagct gacgcgcctg
     1981 gagccgatcc tggcccgcta cccggcccgc gaggagtcgc taatccaggt gctgcagg
//
LOCUS       DAOPQX010000451         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_60897_length_2007_cov_3.325820, whole genome shotgun sequence.
ACCESSION   DAOPQX010000451 DAOPQX010000000
VERSION     DAOPQX010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60897_length_2007_cov_3.325820"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..666
                     /locus_tag="PKK95_16470"
     CDS             55..666
                     /locus_tag="PKK95_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOC19866.1"
                     /translation="MRPATTRQPAVFLDKDGTLVENVPYNVNPARVRLMRGAAEGVAA
                     LHAAGFVLVVVSNQPGVSLGLFEAGALEGVRHRLRELLERAGAPLSGFYFCPHAAGPD
                     GRPLCRCRKPAPGLLLQAARELYLDLGRSWMVGDILDDVEAGRAAGCRTVLVDSGGET
                     EWRLSSARVPHAMAADLKSAAEVVIAGMERVRSWAPRALEAGR"
     gene            668..1813
                     /locus_tag="PKK95_16475"
     CDS             668..1813
                     /locus_tag="PKK95_16475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOC19867.1"
                     /translation="MGAPASGSWEAARNILCVRLDALGDVLMTTPAIRALKERPAGRR
                     ATLLTSSAGASIARLVPEIDEVLVYDAPWVKATPPREGPPADAAMIECLRAKRFDAAV
                     VFTVYSQNPLPAALACYLAAIPLRLAHCRENPYQLLTDWVREPEPERIVRHEVRRQLD
                     LVAEVGAATRDERMSLTVPPGLEEGVRRRLSRLGIDPSGKFVVMHPGATAPSRRYPPE
                     LFAEAASQLVRAHRCPVVFTGGRDEIALVHAVRRSMTARSRSLAGRLTLGELAALLRA
                     APVLVSNNSGPVHMAAALGTPVVDIYALTNPQHTPWMTPHRLLFHDVPCRYCYKSVCP
                     QGHQDCVRLVPPSAVAEAAIELLEGREPRGVPSLPALGPRIRATLED"
     gene            1817..>2007
                     /locus_tag="PKK95_16480"
     CDS             1817..>2007
                     /locus_tag="PKK95_16480"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HOC19868.1"
                     /translation="MARPILTLGINAAFHDPAACLVSDGAVVAAAEDERFTHVKHGKR
                     PIPFTAWELPFHAIDYCLR"
BASE COUNT          240 a          740 c          725 g          302 t
ORIGIN      
        1 tgattcccga gttgatggtg ctgcccatgc gcgagacctc ctggccgtga cgtcatgcgc
       61 ccggccacga cgcgtcagcc ggcggtgttc ctcgacaagg acggcacgct cgtcgagaac
      121 gtgccgtaca acgtcaaccc tgcgcgcgtg aggctcatgc ggggggccgc cgagggggtg
      181 gccgcgctgc acgccgccgg cttcgtgctg gtggtcgtct cgaaccagcc cggcgtcagc
      241 ctcggccttt tcgaggccgg ggcgctcgag ggcgttcgcc accgcctgcg cgagctgctc
      301 gagcgggcgg gcgcgccgct ttccggcttc tacttctgcc cgcacgccgc cggccccgat
      361 ggccgcccgc tgtgccggtg ccgcaagccg gcgccaggcc tgctgctgca ggccgcgcgc
      421 gagctgtatc tcgatctcgg ccggtcgtgg atggtgggcg acatcctcga cgacgtggag
      481 gccggccgcg cggcgggatg ccgaacggtg ctggtggaca gcggtggaga gaccgagtgg
      541 cgcctgtcgt ccgcgcgcgt gccgcacgcg atggcggcgg acctgaagtc ggcggcggag
      601 gtcgtgattg cgggcatgga gcgggtccgg tcgtgggcgc cgcgggcgct cgaggcgggg
      661 aggtagcgtg ggggcccccg cgtccggctc gtgggaggcc gcccggaaca tcctctgcgt
      721 ccggctcgac gcgctgggcg acgtcctgat gacgaccccg gcgatccgcg ccctcaagga
      781 acggcccgct ggccggcgcg cgacgcttct cacctcgtcc gcgggtgcat ccatcgcgcg
      841 gctggttccc gagatcgacg aggtgctggt gtacgacgcg ccctgggtga aggccacgcc
      901 tcctcgtgag ggcccgcccg ccgacgccgc gatgatcgag tgcctgcgcg caaagcggtt
      961 cgacgcggcc gtggtgttca cggtctacag ccagaacccg ttgccggccg ccctcgcctg
     1021 ctacctggcc gccatcccgc ttcgcctcgc tcactgccgc gagaacccct accagctcct
     1081 taccgactgg gtgagggagc cggagcccga gcggatcgtc cggcacgagg tgcgccgcca
     1141 gctcgacctg gtggccgagg tgggcgcggc cacgcgcgac gagcgcatgt cgctcacggt
     1201 tccccccggc ctggaggagg gggtgcgccg gcggctctcg agactcggga tcgacccgtc
     1261 cgggaagttc gtggtgatgc acccgggggc caccgcgccg tcgcgccgct acccgcccga
     1321 gctcttcgcc gaagccgcga gccagttggt ccgcgcccac cggtgcccgg tcgtcttcac
     1381 cggtggacgc gacgagatcg cgctggttca cgccgttcgc cggtcgatga ccgcgcgctc
     1441 gcggtcgctg gccggccgcc tgacgctggg ggagcttgcc gcgctgctgc gcgcggcgcc
     1501 tgtgctcgtc tcgaacaact ccggccccgt gcacatggcc gccgcgctcg gcacgcccgt
     1561 ggtggacatc tacgcgctga ccaacccgca gcacaccccg tggatgacgc cgcaccggtt
     1621 gctgttccac gacgtccctt gccgctactg ctacaagagc gtctgcccgc aggggcacca
     1681 ggactgcgtg cggctggtgc cgccgtccgc ggttgccgag gctgcgatcg agctgctgga
     1741 agggcgcgag ccgcgggggg ttccaagcct gccggccctc ggcccgcgca ttcgcgccac
     1801 cctcgaggac tgacccatgg cccgaccgat tctcaccctc ggcatcaacg cggcgtttca
     1861 cgacccggcc gcctgcctgg tgtcggacgg ggccgtcgtc gctgccgccg aggacgagcg
     1921 cttcacgcac gtcaagcacg gcaagcgccc gatcccgttc accgcctggg agctgccgtt
     1981 ccacgcgatc gactactgcc tgcgcga
//
LOCUS       DAOPQX010000452         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Vicinamibacterales bacterium isolate AMR_MDS_1511
            NODE_62598_length_1965_cov_3.613089, whole genome shotgun sequence.
ACCESSION   DAOPQX010000452 DAOPQX010000000
VERSION     DAOPQX010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854716
            Sequence Read Archive: SRR4236664
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Vicinamibacterales bacterium (wastewater metagenome)
  ORGANISM  Vicinamibacterales bacterium
            Bacteria; Acidobacteriota; Vicinamibacteria; Vicinamibacterales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 23:10:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,298
            CDSs (total)                      :: 3,271
            Genes (coding)                    :: 3,258
            CDSs (with protein)               :: 3,258
            Genes (RNA)                       :: 27
            tRNAs                             :: 24
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 0 of 13
            Pseudo Genes (incomplete)         :: 13 of 13
            Pseudo Genes (internal stop)      :: 0 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Vicinamibacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62598_length_1965_cov_3.613089"
                     /isolate="AMR_MDS_1511"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2910147"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.53 N 90.43 W"
                     /collection_date="2014-12-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="PKK95_16485"
     CDS             complement(<1..1474)
                     /locus_tag="PKK95_16485"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOC19869.1"
                     /translation="MANLVLGRGRPIVLVLLAAGLGALAALWVERAGPFAPRAGRLTA
                     RVVDDATGAALAARVAVTDASGRPLEVGGARGQVEQLGKRWSYVDAAFTLEVPAGGAT
                     IEIRRGIETRPLVERVSGGTGEQPAQRTFRLRRWANLREAGYAGGDIHAHLPALSTAP
                     LEMRAEDLHALNLLVLGGMPLPNDGSFTGRPDTRSSPEHQIHLSQEIIDWHLGHLTLA
                     GIKSLVPGYPVPCGTLEYWTSHPHCDVLAGGRAARGQPALVSLAHFENLPGAATPVAV
                     ALGLLDALEIPTWSDPMQLPAHLSSWTTSGMPAADFTPMPGIDLYYQFLNAGFRLPIA
                     AGTDKSGEDIPIGSNRVYARTKGADGFAAWLEGIKEGSGFVTNGPLLEFDVEGATPGD
                     VVAFDGNRTVRVRAAARSILPFTTLEIVMNGRVVAHTLKLVQANAPVDGVYTLQTEAT
                     LTLQLSGWLAARAFADPDITPRLLPRGSSVFAHTNPVYFLQ"
     gene            complement(1514..>1965)
                     /locus_tag="PKK95_16490"
     CDS             complement(1514..>1965)
                     /locus_tag="PKK95_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOC19870.1"
                     /translation="EGQVMKVLHDHFAGALPVAVDGTSPQVASKGVVGIDNSARPSGS
                     PTYPLDVFAALSADRRTIAVSVVNPSEAPQECDLNLRGVEAKGPAKVSQLTAPDTPPP
                     APGPPGMGRFGGPPVSVSQSTLPQPPRRVTVPPTSITVYQFEVRAIS"
BASE COUNT          293 a          659 c          720 g          293 t
ORIGIN      
        1 cctgcaggaa gtagaccggg ttcgtgtgcg cgaacacgga cgacccgcgc ggcagcagcc
       61 tcggggtgat gtcggggtcg gcgaaggcgc gcgccgcaag ccagccactc agctggagcg
      121 tcagagttgc ctctgtctgc agcgtgtaga cgccgtcgac cggggcgttg gcctgcacga
      181 gcttgagggt gtgcgcgacc acccggccgt tcatcacgat ctcgagggtc gtgaagggca
      241 ggatcgaccg ggcggcggcg cgcaccctca ccgttctgtt cccatcgaat gcgaccacgt
      301 cccccggggt tgcaccctcc acgtcgaact cgagaagcgg cccgttggtc acgaaccccg
      361 agccctcttt gattccctcg agccacgcgg cgaagccgtc tgcacccttc gtacgggcgt
      421 agacgcggtt gctgccgatc gggatgtcct cgcccgactt gtcggtcccg gcggcaatcg
      481 gcagccggaa accggcgttc aggaactggt agtagagatc gatgccgggc atcggggtga
      541 agtcggccgc gggcattccg gaggtggtcc aggaggagag gtgcgccggg agctgcatgg
      601 gatccgacca ggtcgggatc tccagcgcgt cgagaagtcc gagggccacg gcgaccggcg
      661 tggcggcgcc gggcaggttc tcgaagtgcg ccagcgagac gagggccggc tggccgcggg
      721 ccgcacgccc gccggccagg acgtcgcagt ggggatggct ggtccagtac tcgagcgttc
      781 cgcacggtac ggggtagccg ggcacgaggc tcttgatgcc ggccagcgtg aggtggccga
      841 gatgccagtc gatgatctcc tggctcaggt ggatttggtg ctcgggcgac gagcgggtgt
      901 cgggacgtcc cgtgaacgat ccgtcgttgg gaaggggcat tccgccgagg acgaggaggt
      961 tcagcgcgtg aaggtcctct gctcgcatct cgagaggggc ggtggagagt gctggaaggt
     1021 gcgcgtggat gtcgccgccc gcgtaaccgg cctcccgcag gtttgcccag cgacgcaggc
     1081 ggaacgttct ctgcgcgggc tgctcgccgg tgccgcctga aacccgctcg acgagagggc
     1141 gggtctcaat cccccggcgg atctcgatcg tcgcgccgcc ggccggcacc tcgagggtga
     1201 acgcggcgtc gacgtaggac cagcgcttgc caagctgctc cacctgcccg cgcgcgcctc
     1261 cgacctccag cggcctgccg ctcgcgtcgg tgacggcgac acgcgcggcc agcgcggccc
     1321 cggtggcatc gtccacgacg cgtgccgtga gcctcccggc gcgcggcgcg aacgggccgg
     1381 cgcgctccac ccacagcgcg gccagcgcgc cgagacccgc cgcgagcagg accagaacga
     1441 ttggacggcc tcgcccgaga acgagattcg ccatatcttc ctccgggggg cgggctacat
     1501 cccaaaacgc ggctcaggag atcgccctga cctcgaactg gtagaccgtg atgctcgtcg
     1561 gcggcacggt cactcgtcgc gggggctgcg gcagcgtact ctgtgacaca ctgaccggcg
     1621 gcccgccgaa gcggcccatg cccggagggc caggcgctgg cggcggcgtg tcgggcgcgg
     1681 tcagctgcga cacttttgcg ggacccttcg cctccacgcc ccttaggttc aggtcgcact
     1741 cctgcggcgc ttcagagggg ttcacgaccg agaccgcgat cgtccggcga tcggcgctca
     1801 gggccgcaaa cacatcgagc gggtacgtgg ggctgccgga gggtcgcgcc gagttgtcga
     1861 tcccgaccac tcccttcgac gcgacttgcg gcgaggtgcc gtcgaccgcg acggggaggg
     1921 cgcccgcgaa gtggtcgtgc agcaccttca tgacctgtcc ttcca
//
